Sample records for force redevelopment kinetics

  1. The cross-bridge dynamics is determined by two length-independent kinetics: Implications on muscle economy and Frank-Starling Law.

    PubMed

    Amiad Pavlov, Daria; Landesberg, Amir

    2016-01-01

    The cellular mechanisms underlying the Frank-Starling Law of the heart and the skeletal muscle force-length relationship are not clear. This study tested the effects of sarcomere length (SL) on the average force per cross-bridge and on the rate of cross-bridge cycling in intact rat cardiac trabeculae (n=9). SL was measured by laser diffraction and controlled with a fast servomotor to produce varying initial SLs. Tetanic contractions were induced by addition of cyclopiazonic acid, to maintain a constant activation. Stress decline and redevelopment in response to identical ramp shortenings, starting at various initial SLs, was analyzed. Both stress decline and redevelopment responses revealed two distinct kinetics: a fast and a slower phase. The duration of the rapid phases (4.2 ± 0.1 msec) was SL-independent. The second slower phase depicted a linear dependence of the rate of stress change on the instantaneous stress level. Identical slopes (70.5 ± 1.6 [1/s], p=0.33) were obtained during ramp shortening at all initial SLs, indicating that the force per cross-bridge and cross-bridge cycling kinetics are length-independent. A decrease in the slope at longer SLs was obtained during stress redevelopment, due to internal shortening. The first phase is attributed to rapid changes in the average force per cross-bridge. The second phase is ascribed to both cross-bridge cycling between its strong and weak conformations and to changes in the number of strong cross-bridges. Cross-bridge cycling kinetics and muscle economy are length-independent and the Frank-Starling Law cannot be attributed to changes in the force per cross-bridge or in the single cross-bridge cycling rates. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Revisiting Frank-Starling: regulatory light chain phosphorylation alters the rate of force redevelopment (ktr ) in a length-dependent fashion.

    PubMed

    Toepfer, Christopher N; West, Timothy G; Ferenczi, Michael A

    2016-09-15

    Regulatory light chain (RLC) phosphorylation has been shown to alter the ability of muscle to produce force and power during shortening and to alter the rate of force redevelopment (ktr ) at submaximal [Ca(2+) ]. Increasing RLC phosphorylation ∼50% from the in vivo level in maximally [Ca(2+) ]-activated cardiac trabecula accelerates ktr . Decreasing RLC phosphorylation to ∼70% of the in vivo control level slows ktr and reduces force generation. ktr is dependent on sarcomere length in the physiological range 1.85-1.94 μm and RLC phosphorylation modulates this response. We demonstrate that Frank-Starling is evident at maximal [Ca(2+) ] activation and therefore does not necessarily require length-dependent change in [Ca(2+) ]-sensitivity of thin filament activation. The stretch response is modulated by changes in RLC phosphorylation, pinpointing RLC phosphorylation as a modulator of the Frank-Starling law in the heart. These data provide an explanation for slowed systolic function in the intact heart in response to RLC phosphorylation reduction. Force and power in cardiac muscle have a known dependence on phosphorylation of the myosin-associated regulatory light chain (RLC). We explore the effect of RLC phosphorylation on the ability of cardiac preparations to redevelop force (ktr ) in maximally activating [Ca(2+) ]. Activation was achieved by rapidly increasing the temperature (temperature-jump of 0.5-20ºC) of permeabilized trabeculae over a physiological range of sarcomere lengths (1.85-1.94 μm). The trabeculae were subjected to shortening ramps over a range of velocities and the extent of RLC phosphorylation was varied. The latter was achieved using an RLC-exchange technique, which avoids changes in the phosphorylation level of other proteins. The results show that increasing RLC phosphorylation by 50% accelerates ktr by ∼50%, irrespective of the sarcomere length, whereas decreasing phosphorylation by 30% slows ktr by ∼50%, relative to the ktr obtained for in vivo phosphorylation. Clearly, phosphorylation affects the magnitude of ktr following step shortening or ramp shortening. Using a two-state model, we explore the effect of RLC phosphorylation on the kinetics of force development, which proposes that phosphorylation affects the kinetics of both attachment and detachment of cross-bridges. In summary, RLC phosphorylation affects the rate and extent of force redevelopment. These findings were obtained in maximally activated muscle at saturating [Ca(2+) ] and are not explained by changes in the Ca(2+) -sensitivity of acto-myosin interactions. The length-dependence of the rate of force redevelopment, together with the modulation by the state of RLC phosphorylation, suggests that these effects play a role in the Frank-Starling law of the heart. © 2016 Wellcome Trust The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.

  3. Kinetic coupling of phosphate release, force generation and rate-limiting steps in the cross-bridge cycle.

    PubMed

    Stehle, Robert; Tesi, Chiara

    2017-08-01

    A basic goal in muscle research is to understand how the cyclic ATPase activity of cross-bridges is converted into mechanical force. A direct approach to study the chemo-mechanical coupling between P i release and the force-generating step is provided by the kinetics of force response induced by a rapid change in [P i ]. Classical studies on fibres using caged-P i discovered that rapid increases in [P i ] induce fast force decays dependent on final [P i ] whose kinetics were interpreted to probe a fast force-generating step prior to P i release. However, this hypothesis was called into question by studies on skeletal and cardiac myofibrils subjected to P i jumps in both directions (increases and decreases in [P i ]) which revealed that rapid decreases in [P i ] trigger force rises with slow kinetics, similar to those of calcium-induced force development and mechanically-induced force redevelopment at the same [P i ]. A possible explanation for this discrepancy came from imaging of individual sarcomeres in cardiac myofibrils, showing that the fast force decay upon increase in [P i ] results from so-called sarcomere 'give'. The slow force rise upon decrease in [P i ] was found to better reflect overall sarcomeres cross-bridge kinetics and its [P i ] dependence, suggesting that the force generation coupled to P i release cannot be separated from the rate-limiting transition. The reasons for the different conclusions achieved in fibre and myofibril studies are re-examined as the recent findings on cardiac myofibrils have fundamental consequences for the coupling between P i release, rate-limiting steps and force generation. The implications from P i -induced force kinetics of myofibrils are discussed in combination with historical and recent models of the cross-bridge cycle.

  4. The ATP hydrolysis and phosphate release steps control the time course of force development in rabbit skeletal muscle.

    PubMed

    Sleep, John; Irving, Malcolm; Burton, Kevin

    2005-03-15

    The time course of isometric force development following photolytic release of ATP in the presence of Ca(2+) was characterized in single skinned fibres from rabbit psoas muscle. Pre-photolysis force was minimized using apyrase to remove contaminating ATP and ADP. After the initial force rise induced by ATP release, a rapid shortening ramp terminated by a step stretch to the original length was imposed, and the time course of the subsequent force redevelopment was again characterized. Force development after ATP release was accurately described by a lag phase followed by one or two exponential components. At 20 degrees C, the lag was 5.6 +/- 0.4 ms (s.e.m., n = 11), and the force rise was well fitted by a single exponential with rate constant 71 +/- 4 s(-1). Force redevelopment after shortening-restretch began from about half the plateau force level, and its single-exponential rate constant was 68 +/- 3 s(-1), very similar to that following ATP release. When fibres were activated by the addition of Ca(2+) in ATP-containing solution, force developed more slowly, and the rate constant for force redevelopment following shortening-restretch reached a maximum value of 38 +/- 4 s(-1) (n = 6) after about 6 s of activation. This lower value may be associated with progressive sarcomere disorder at elevated temperature. Force development following ATP release was much slower at 5 degrees C than at 20 degrees C. The rate constant of a single-exponential fit to the force rise was 4.3 +/- 0.4 s(-1) (n = 22), and this was again similar to that after shortening-restretch in the same activation at this temperature, 3.8 +/- 0.2 s(-1). We conclude that force development after ATP release and shortening-restretch are controlled by the same steps in the actin-myosin ATPase cycle. The present results and much previous work on mechanical-chemical coupling in muscle can be explained by a kinetic scheme in which force is generated by a rapid conformational change bracketed by two biochemical steps with similar rate constants -- ATP hydrolysis and the release of inorganic phosphate -- both of which combine to control the rate of force development.

  5. A cross-bridge based model of force depression: Can a single modification address both transient and steady-state behaviors?

    PubMed

    Corr, David T; Herzog, Walter

    2016-03-21

    Force depression (FD), the reduction of isometric force following active shortening, is a phenomenon of skeletal muscle that has received significant attention in biomechanical and physiological literature, yet the mechanisms underlying FD remain unknown. Recent experiments identified a slower rate of force redevelopment with increasing amounts of steady-state FD, suggesting that FD may be caused, at least in part, by a decrease in cross-bridge binding rate (Corr and Herzog, 2005; Koppes et al., 2014). Herein, we develop a cross-bridge based model of FD in which the binding rate function, f, decreases with the mechanical work performed during shortening. This modification incorporates a direct relationship between steady-state FD and muscle mechanical work (Corr and Herzog, 2005; Herzog et al., 2000; Kosterina et al., 2008), and is consistent with a proposed mechanism attributing FD to stress-induced inhibition of cross-bridge attachments (Herzog, 1998; Maréchal and Plaghki, 1979). Thus, for an increase in mechanical work, the model should predict a slower force redevelopment (decreased attachment rate) to a more depressed steady-state force (fewer attached cross-bridges), and a reduction in contractile element stiffness (Ford et al., 1981). We hypothesized that since this modification affects the cross-bridge kinetics, a corresponding model would be able to account for both transient and steady-state FD behaviors. Comparisons to prior experiments (Corr and Herzog, 2005; Herzog et al., 2000; Kosterina et al., 2008) show that both steady-state and transient aspects of FD, as well as the relationship of FD with respect to speed and amplitude of shortening, are well captured by this model. Thus, this relatively simple cross-bridge based model of FD lends support to a mechanism involving the inhibition of cross-bridge binding, and indicates that cross-bridge kinetics may play a critical role in FD. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. 75 FR 29322 - Base Closure and Realignment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-25

    ...-6020. Local Redevelopment Authority (LRA) Arizona Installation Name: Air Force Research Laboratory. LRA... provides a corresponding listing of the Local Redevelopment Authorities (LRA) for the Air Force Research Laboratory, Arizona, and portions of the Newport Naval Complex, Rhode Island, recognized by the Secretary of...

  7. Isotonic force modulates force redevelopment rate of intact frog muscle fibres: evidence for cross-bridge induced thin filament activation

    PubMed Central

    Vandenboom, Rene; Hannon, James D; Sieck, Gary C

    2002-01-01

    We tested the hypothesis that force-velocity history modulates thin filament activation, as assessed by the rate of force redevelopment after shortening (+dF/dtR). The influence of isotonic force on +dF/dtR was assessed by imposing uniform amplitude (2.55 to 2.15 μm sarcomere−1) but different speed releases to intact frog muscle fibres during fused tetani. Each release consisted of a contiguous ramp- and step-change in length. Ramp speed was changed from release to release to vary fibre shortening speed from 1.00 (2.76 ± 0.11 μm half-sarcomere−1 s−1) to 0.30 of maximum unloaded shortening velocity (Vu), thereby modulating isotonic force from 0 to 0.34 Fo, respectively. The step zeroed force and allowed the fibre to shorten unloaded for a brief period of time prior to force redevelopment. Although peak force redevelopment after different releases was similar, +dF/dtR increased by 81 ± 6% (P < 0.05) as fibre shortening speed was reduced from 1.00 Vu. The +dF/dtR after different releases was strongly correlated with the preceding isotonic force (r = 0.99, P < 0.001). Results from additional experiments showed that the slope of slack test plots produced by systematically increasing the step size that followed each ramp were similar. Thus, isotonic force did not influence Vu (mean: 2.84 ± 0.10 μm half-sarcomere−1 s−1, P < 0.05). We conclude that isotonic force modulates +dF/dtR independent of change in Vu, an outcome consistent with a cooperative influence of attached cross-bridges on thin filament activation that increases cross-bridge attachment rate without alteration to cross-bridge detachment rate. PMID:12205189

  8. Kinetics of force recovery following length changes in active skinned single fibres from rabbit psoas muscle

    PubMed Central

    Burton, Kevin; Simmons, Robert M; Sleep, John; Smith, David A

    2006-01-01

    Redevelopment of isometric force following shortening of skeletal muscle is thought to result from a redistribution of cross-bridge states. We varied the initial force and cross-bridge distribution by applying various length-change protocols to active skinned single fibres from rabbit psoas muscle, and observed the effect on the slowest phase of recovery (‘late recovery’) that follows transient changes. In response to step releases that reduced force to near zero (∼8 nm (half sarcomere)−1) or prolonged shortening at high velocity, late recovery was well described by two exponentials of approximately equal amplitude and rate constants of ∼2 s−1 and ∼9 s−1 at 5°C. When a large restretch was applied at the end of rapid shortening, recovery was accelerated by (1) the introduction of a slow falling component that truncated the rise in force, and (2) a relative increase in the contribution of the fast exponential component. The rate of the slow fall was similar to that observed after a small isometric step stretch, with a rate of 0.4–0.8 s−1, and its effects could be reversed by reducing force to near zero immediately after the stretch. Force at the start of late recovery was varied in a series of shortening steps or ramps in order to probe the effect of cross-bridge strain on force redevelopment. The rate constants of the two components fell by 40–50% as initial force was raised to 75–80% of steady isometric force. As initial force increased, the relative contribution of the fast component decreased, and this was associated with a length constant of about 2 nm. The results are consistent with a two-state strain-dependent cross-bridge model. In the model there is a continuous distribution of recovery rate constants, but two-exponential fits show that the fast component results from cross-bridges initially at moderate positive strain and the slow component from cross-bridges at high positive strain. PMID:16497718

  9. Effect of muscle length on cross-bridge kinetics in intact cardiac trabeculae at body temperature.

    PubMed

    Milani-Nejad, Nima; Xu, Ying; Davis, Jonathan P; Campbell, Kenneth S; Janssen, Paul M L

    2013-01-01

    Dynamic force generation in cardiac muscle, which determines cardiac pumping activity, depends on both the number of sarcomeric cross-bridges and on their cycling kinetics. The Frank-Starling mechanism dictates that cardiac force development increases with increasing cardiac muscle length (corresponding to increased ventricular volume). It is, however, unclear to what extent this increase in cardiac muscle length affects the rate of cross-bridge cycling. Previous studies using permeabilized cardiac preparations, sub-physiological temperatures, or both have obtained conflicting results. Here, we developed a protocol that allowed us to reliably and reproducibly measure the rate of tension redevelopment (k(tr); which depends on the rate of cross-bridge cycling) in intact trabeculae at body temperature. Using K(+) contractures to induce a tonic level of force, we showed the k(tr) was slower in rabbit muscle (which contains predominantly β myosin) than in rat muscle (which contains predominantly α myosin). Analyses of k(tr) in rat muscle at optimal length (L(opt)) and 90% of optimal length (L(90)) revealed that k(tr) was significantly slower at L(opt) (27.7 ± 3.3 and 27.8 ± 3.0 s(-1) in duplicate analyses) than at L(90) (45.1 ± 7.6 and 47.5 ± 9.2 s(-1)). We therefore show that k(tr) can be measured in intact rat and rabbit cardiac trabeculae, and that the k(tr) decreases when muscles are stretched to their optimal length under near-physiological conditions, indicating that the Frank-Starling mechanism not only increases force but also affects cross-bridge cycling kinetics.

  10. Novel approaches to determine contractile function of the isolated adult zebrafish ventricular cardiac myocyte.

    PubMed

    Dvornikov, Alexey V; Dewan, Sukriti; Alekhina, Olga V; Pickett, F Bryan; de Tombe, Pieter P

    2014-05-01

    The zebrafish (Danio rerio) has been used extensively in cardiovascular biology, but mainly in the study of heart development. The relative ease of its genetic manipulation may indicate the suitability of this species as a cost-effective model system for the study of cardiac contractile biology. However, whether the zebrafish heart is an appropriate model system for investigations pertaining to mammalian cardiac contractile structure-function relationships remains to be resolved. Myocytes were isolated from adult zebrafish hearts by enzymatic digestion, attached to carbon rods, and twitch force and intracellular Ca(2+) were measured. We observed the modulation of twitch force, but not of intracellular Ca(2+), by both extracellular [Ca(2+)] and sarcomere length. In permeabilized cells/myofibrils, we found robust myofilament length-dependent activation. Moreover, modulation of myofilament activation-relaxation and force redevelopment kinetics by varied Ca(2+) activation levels resembled that found previously in mammalian myofilaments. We conclude that the zebrafish is a valid model system for the study of cardiac contractile structure-function relationships.

  11. Sex dimorphisms of crossbridge cycling kinetics in transgenic hypertrophic cardiomyopathy mice.

    PubMed

    Birch, Camille L; Behunin, Samantha M; Lopez-Pier, Marissa A; Danilo, Christiane; Lipovka, Yulia; Saripalli, Chandra; Granzier, Henk; Konhilas, John P

    2016-07-01

    Familial hypertrophic cardiomyopathy (HCM) is a disease of the sarcomere and may lead to hypertrophic, dilated, restrictive, and/or arrhythmogenic cardiomyopathy, congestive heart failure, or sudden cardiac death. We hypothesized that hearts from transgenic HCM mice harboring a mutant myosin heavy chain increase the energetic cost of contraction in a sex-specific manner. To do this, we assessed Ca(2+) sensitivity of tension and crossbridge kinetics in demembranated cardiac trabeculas from male and female wild-type (WT) and HCM hearts at an early time point (2 mo of age). We found a significant effect of sex on Ca(2+) sensitivity such that male, but not female, HCM mice displayed a decrease in Ca(2+) sensitivity compared with WT counterparts. The HCM transgene and sex significantly impacted the rate of force redevelopment by a rapid release-restretch protocol and tension cost by the ATPase-tension relationship. In each of these measures, HCM male trabeculas displayed a gain-of-function when compared with WT counterparts. In addition, cardiac remodeling measured by echocardiography, histology, morphometry, and posttranslational modifications demonstrated sex- and HCM-specific effects. In conclusion, female and male HCM mice display sex dimorphic crossbridge kinetics accompanied by sex- and HCM-dependent cardiac remodeling at the morphometric, histological, and cellular level. Copyright © 2016 the American Physiological Society.

  12. 75 FR 33587 - Local Redevelopment Authority and Available Surplus Buildings and Land at Air Force Research Labs...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-14

    ... Surplus Buildings and Land at Air Force Research Labs (AFRL) Mesa, Located in Maricopa County, AZ SUMMARY: This notice provides information regarding the surplus property at AFRL Mesa in Maricopa County... to plan the reuse of the AFRL Mesa property. The property is located within the former Williams Air...

  13. Differences in Contractile Function of Myofibrils within Human Embryonic Stem Cell-Derived Cardiomyocytes vs. Adult Ventricular Myofibrils Are Related to Distinct Sarcomeric Protein Isoforms

    PubMed Central

    Iorga, Bogdan; Schwanke, Kristin; Weber, Natalie; Wendland, Meike; Greten, Stephan; Piep, Birgit; dos Remedios, Cristobal G.; Martin, Ulrich; Zweigerdt, Robert; Kraft, Theresia; Brenner, Bernhard

    2018-01-01

    Characterizing the contractile function of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is key for advancing their utility for cellular disease models, promoting cell based heart repair, or developing novel pharmacological interventions targeting cardiac diseases. The aim of the present study was to understand whether steady-state and kinetic force parameters of β-myosin heavy chain (βMyHC) isoform-expressing myofibrils within human embryonic stem cell-derived cardiomyocytes (hESC-CMs) differentiated in vitro resemble those of human ventricular myofibrils (hvMFs) isolated from adult donor hearts. Contractile parameters were determined using the same micromechanical method and experimental conditions for both types of myofibrils. We identified isoforms and phosphorylation of main sarcomeric proteins involved in the modulation of force generation of both, chemically demembranated hESC-CMs (d-hESC-CMs) and hvMFs. Our results indicate that at saturating Ca2+ concentration, both human-derived contractile systems developed forces with similar rate constants (0.66 and 0.68 s−1), reaching maximum isometric force that was significantly smaller for d-hESC-CMs (42 kPa) than for hvMFs (94 kPa). At submaximal Ca2+-activation, where intact cardiomyocytes normally operate, contractile parameters of d-hESC-CMs and hvMFs exhibited differences. Ca2+ sensitivity of force was higher for d-hESC-CMs (pCa50 = 6.04) than for hvMFs (pCa50 = 5.80). At half-maximum activation, the rate constant for force redevelopment was significantly faster for d-hESC-CMs (0.51 s−1) than for hvMFs (0.28 s−1). During myofibril relaxation, kinetics of the slow force decay phase were significantly faster for d-hESC-CMs (0.26 s−1) than for hvMFs (0.21 s−1), while kinetics of the fast force decay were similar and ~20x faster. Protein analysis revealed that hESC-CMs had essentially no cardiac troponin-I, and partially non-ventricular isoforms of some other sarcomeric proteins, explaining the functional discrepancies. The sarcomeric protein isoform pattern of hESC-CMs had features of human cardiomyocytes at an early developmental stage. The study indicates that morphological and ultrastructural maturation of βMyHC isoform-expressing hESC-CMs is not necessarily accompanied by ventricular-like expression of all sarcomeric proteins. Our data suggest that hPSC-CMs could provide useful tools for investigating inherited cardiac diseases affecting contractile function during early developmental stages. PMID:29403388

  14. Environmental Assessment for Proposed Construction Freight Transfer Facility, Buckley Air Force Base, Colorado

    DTIC Science & Technology

    2007-06-01

    construction at the Fitzsimons Redevelopment area. As a result, there could be a negligible cumulative adverse impact on the ambient soundscape . The... soundscape in conjunction with other plans and projects. However, the attenuation of sound over distance and the phasing of development activity

  15. Slowed Relaxation in Fatigued Skeletal Muscle Fibers of Xenopus and Mouse

    PubMed Central

    Westerblad, Håkan; Lännergren, Jan; Allen, David G.

    1997-01-01

    Slowing of relaxation is an important characteristic of skeletal muscle fatigue. The aim of the present study was to quantify the relative contribution of altered Ca2+ handling (calcium component) and factors down-stream to Ca2+ (cross-bridge component) to the slowing of relaxation in fatigued fibers of Xenopus and mouse. Two types of Xenopus fibers were used: easily fatigued, type 1 fibers and fatigue resistant, type 2 fibers. In these Xenopus fibers the free myoplasmic [Ca2+] ([Ca2+]i) was measured with indo-1, and the relaxation of Ca2+-derived force, constructed from tetanic [Ca2+]i records and in vivo [Ca2+]i-force curves, was analyzed. An alternative method was used in both Xenopus and mouse fibers: fibers were rapidly shortened during the initial phase of relaxation, and the time to the peak of force redevelopment was measured. These two methods gave similar results and showed proportional slowing of the calcium and cross-bridge components of relaxation in both fatigued type 1 and type 2 Xenopus fibers, whereas only the cross-bridge component was slowed in fatigued mouse fibers. Ca2+ removal from the myoplasm during relaxation was markedly less effective in Xenopus fibers as compared to mouse fibers. Fatigued Xenopus fibers displayed a reduced rate of sarcoplasmic reticulum Ca2+ uptake and increased sarcoplasmic reticulum Ca2+ leak. Some fibers were stretched at various times during relaxation. The resistance to these stretches was increased during fatigue, especially in Xenopus fibers, which indicates that longitudinal movements during relaxation had become less pronounced and this might contribute to the increased cross-bridge component of relaxation in fatigue. In conclusion, slowing of relaxation in fatigued Xenopus fibers is caused by impaired Ca2+ handling and altered cross-bridge kinetics, whereas the slowing in mouse fibers is only due to altered cross-bridge kinetics. PMID:9089444

  16. GSK3β phosphorylates newly identified site in the proline-alanine-rich region of cardiac myosin-binding protein C and alters cross-bridge cycling kinetics in human: short communication.

    PubMed

    Kuster, Diederik W D; Sequeira, Vasco; Najafi, Aref; Boontje, Nicky M; Wijnker, Paul J M; Witjas-Paalberends, E Rosalie; Marston, Steven B; Dos Remedios, Cristobal G; Carrier, Lucie; Demmers, Jeroen A A; Redwood, Charles; Sadayappan, Sakthivel; van der Velden, Jolanda

    2013-02-15

    Cardiac myosin-binding protein C (cMyBP-C) regulates cross-bridge cycling kinetics and, thereby, fine-tunes the rate of cardiac muscle contraction and relaxation. Its effects on cardiac kinetics are modified by phosphorylation. Three phosphorylation sites (Ser275, Ser284, and Ser304) have been identified in vivo, all located in the cardiac-specific M-domain of cMyBP-C. However, recent work has shown that up to 4 phosphate groups are present in human cMyBP-C. To identify and characterize additional phosphorylation sites in human cMyBP-C. Cardiac MyBP-C was semipurified from human heart tissue. Tandem mass spectrometry analysis identified a novel phosphorylation site on serine 133 in the proline-alanine-rich linker sequence between the C0 and C1 domains of cMyBP-C. Unlike the known sites, Ser133 was not a target of protein kinase A. In silico kinase prediction revealed glycogen synthase kinase 3β (GSK3β) as the most likely kinase to phosphorylate Ser133. In vitro incubation of the C0C2 fragment of cMyBP-C with GSK3β showed phosphorylation on Ser133. In addition, GSK3β phosphorylated Ser304, although the degree of phosphorylation was less compared with protein kinase A-induced phosphorylation at Ser304. GSK3β treatment of single membrane-permeabilized human cardiomyocytes significantly enhanced the maximal rate of tension redevelopment. GSK3β phosphorylates cMyBP-C on a novel site, which is positioned in the proline-alanine-rich region and increases kinetics of force development, suggesting a noncanonical role for GSK3β at the sarcomere level. Phosphorylation of Ser133 in the linker domain of cMyBP-C may be a novel mechanism to regulate sarcomere kinetics.

  17. SUPPORTING THE REDEVELOPMENT OF BROWNFIELD SITES USING SITE-SPECIFIC MANAGEMENT APPROACHES AND REDEVELOPMENT TOOLS (SMART)

    EPA Science Inventory

    The Site-Specific Management Approaches and Redevelopment Tools (SMART) provides potential solutions for facilitating the redevelopment of brownfield sites. The term "brownfield site" refers to previously developed property whose reuse may be complicated by the presence of hazar...

  18. Altered myocardial force generation in end-stage human heart failure.

    PubMed

    Papp, Zoltán; van der Velden, Jolanda; Borbély, Attila; Édes, István; Stienen, Ger J M

    2014-12-01

    This study aimed to elucidate the molecular background of increased Ca 2+ sensitivity of force production in cardiomyocytes of end-stage human heart failure. Ca 2+ -activated isometric force and the cross-bridge specific rate of force redevelopment (k tr ) were determined in Triton-skinned myocytes from end-stage failing and non-failing donor hearts. Measurements (control: pH 7.2, 0 mM inorganic phosphate (P i )) were performed under test conditions that probed either the Ca 2+ -regulatory function of the thin filaments (pH 6.5), the kinetics of the actin-myosin cross-bridge cycle (10 mM P i ), or both (pH 6.5, 10 mM P i ). The control maximal Ca 2+ -activated force (F o ) and k trmax did not differ between failing and non-failing myocytes. At submaximal [Ca 2+ ], however, both force and k tr were higher in failing than in donor myocytes. The difference in the Ca 2+ sensitivities of force production was preserved when the thin filament regulatory function was perturbed by acidosis (pH 6.5) but was abolished by cross-bridge modulation (i.e. by P i ) both at pH 7.2 and at pH 6.5. P i induced a larger reduction in force but a smaller increase in k tr in the failing myocytes than in the non-failing myocytes at submaximal [Ca 2+ ]. The enhanced P i sensitivity of the actin-myosin interaction suggests that the P i release step of the actin-myosin cross-bridge cycle is modified during end-stage human heart failure. This might be of functional importance when P i accumulates (e.g. during cardiac ischaemia). Moreover, this alteration can influence cardiac energetics and the clinical efficacy of sarcomere targeted agents in human heart failure. © 2015 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology.

  19. Evaluation of Pre- and Post- Redevelopment Groundwater Chemical Analyses from LM Monitoring Wells

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kamp, Susan; Dayvault, Jalena

    This report documents the efforts and analyses conducted for the Applied Studies and Technology (AS&T) Ancillary Work Plan (AWP) project titled Evaluation of Pre- and Post- Redevelopment Groundwater Sample Laboratory Analyses from Selected LM Groundwater Monitoring Wells. This effort entailed compiling an inventory of nearly 500 previous well redevelopment events at 16 U.S. Department of Energy Office of Legacy Management (LM) sites, searching the literature for impacts of well redevelopment on groundwater sample quality, and—the focus of this report—evaluating the impacts of well redevelopment on field measurements and sample analytical results. Study Catalyst Monitoring well redevelopment, the surging or high-volumemore » pumping of a well to loosen and remove accumulated sediment and biological build-up from a well, is considered an element of monitoring well maintenance that is implemented periodically during the lifetime of the well to mitigate its gradual deterioration. Well redevelopment has been conducted fairly routinely at a few LM sites in the western United States (e.g., the Grand Junction office site and the Gunnison processing site in Colorado), but at most other sites in this region it is not a routine practice. Also, until recently (2014–2015), there had been no specific criteria for implementing well redevelopment, and documentation of redevelopment events has been inconsistent. A catalyst for this evaluation was the self-identification of these inconsistencies by the Legacy Management Support contractor. As a result, in early 2015 Environmental Monitoring Operations (EMO) staff began collecting and documenting additional field measurements during well redevelopment events. In late 2015, AS&T staff undertook an independent internal evaluation of EMO's well redevelopment records and corresponding pre- and post-well-redevelopment groundwater analytical results. Study Findings Although literature discussions parallel the prevailing industry-wide assumption that well redevelopment is necessary to increase production or to extend the life of a well, no data in the literature indicate that redevelopment affects chemical signatures in monitoring wells. The comprehensive evaluation undertaken for this study also yielded no evidence that redevelopment has any quantifiable or predictable effect on groundwater sample quality. Both short-term and long-term changes in groundwater chemistry were assessed relative to preceding and subsequent well redevelopment events. Although short-term changes in specific conductance or contaminant concentrations likely attributable to well redevelopment were found at some sites, these cases were limited to a small subset of wells in which chemical stratification had been observed. Redevelopment causes mixing of the well water, resulting in short-term impacts, but not in a consistent direction. Long-term groundwater concentration trends of uranium, the primary contaminant of concern at most LM Uranium Mill Tailings Radiation Control Act (UMTRCA) sites, were evaluated for all 16 sites addressed in this study. Based on the data evaluated, there are no apparent impacts of well redevelopment on uranium trends. In most cases where deviations in trends were found, these could be attributed to water level fluctuations or other factors. A few exceptions were found where spikes or marked decreases in uranium concentrations could potentially be related to the preceding well redevelopment, but apparent impacts were neither predictable nor quantifiable. Summary and Recommendations A catalyst for this project was a concern that there was a need for strict criteria for a program-wide approach to well redevelopment at LM sites. There was also an implicit question as to whether well redevelopment was a necessary practice that was being overlooked at some sites. The primary objective of this study was to determine if there are significant differences in laboratory analytical results between pre- and post-redevelopment groundwater samples. Results of this evaluation indicate that this is not the case—groundwater concentrations of uranium, the primary contaminant of concern at most LM UMTRCA sites, generally remained unchanged pre- and post-well-redevelopment. The literature supports redevelopment of monitoring and municipal wells if signs of reduced productivity, biofouling, sediment buildup, or other conditions potentially affecting long-term well integrity are observed. In these cases, use of a downhole camera to examine the condition of the well screen and casing may be useful. However, based on the data sets examined for this study, there is no evidence that well redevelopment is needed in order to obtain samples that have the same chemical concentrations as those in the groundwater. To conclusively demonstrate that point—that is, to define chemical effects—the underlying mechanisms have to be understood. For example, if biofouling is observed in a well and is considered a potential cause of spurious or invalid chemical data, appropriate hypothesis-testing ethodology should be used to test the validity of this claim. Until late 2014, the onset of this project, there was no standard procedure for documenting well redevelopment events; some (perhaps many) had not been captured in the historical record. EMO has made notable progress in this regard since 2015, having established a format for documenting well redevelopment events and associated field measurements, as well as a data repository for capturing those records. This policy should be continued to ensure that all well redevelopment events and associated field observations are recorded and easily tracked.« less

  20. Myrtle Beach Air Force Base Community Redevelopment Plan

    DTIC Science & Technology

    1993-01-01

    Potential U ses .......................................... 3-2 3-2 Ranking of Potential Uses ....................................... 3-3 3-3 Tourism M...economy has been primarily resort and tourism - and, to a lesser degree, retirement. During the 1980’s, the Grand Strand experienced substantial...economic orientation toward tourism . Last year, the Grand Strand generated $1.7 billion in total tourism spending from the tourist market. These visitors

  1. Finding of No Significant Impact/Finding of No Practicable Alternative: Environmental Assessment of the Privatization of Military Family Housing at MacDill Air Force Base, Florida

    DTIC Science & Technology

    2007-10-09

    specifically, the eastern portion of MacDill AFB, near the MFH parcels. The essence of the Proposed Action is redevelopment (i.e., demolition, road...Protection Attn: Jasmine Ruffington 3900 Commonwealth Blvd., MS 47 Tallahassee, FL 32399-3000 Florida State Clearinghouse Department of Environmental

  2. “Everything that looks good ain’t good!”: Perspectives on Urban Redevelopment among Persons with a History of Injection Drug Use in Baltimore, Maryland

    PubMed Central

    Linton, Sabriya L.; Kennedy, Caitlin E.; Latkin, Carl A.; Celentano, David D.; Kirk, Gregory D.; Mehta, Shruti H.

    2013-01-01

    Background While urban redevelopment is intended to ameliorate urban decay, some studies demonstrate that it can negatively impact some residents. Few studies have considered its impact on persons with a history of drug use. Methods A convenience sample of 25 current or former injection drug users from Baltimore, Maryland, enrolled in the AIDS Linked to the Intravenous Experience study, and reporting residence in or bordering a redeveloping neighborhood participated in 1-2 semi-structured in-depth interviews from July, 2011-February, 2012. Interviews explored personal experiences with redevelopment and perceptions of community-wide impact. Data were analyzed using the constant comparison method. Results Respondents rarely described urban redevelopment as solely negative or positive. Revitalization and increased security in the redeveloping area were reported as positive consequences. Negative consequences included the lack of redevelopment-related employment opportunities, disruption of social ties, and housing instability among relocated residents. Respondents also said that urban redevelopment led to the displacement of drug markets to adjacent neighborhoods and outlying counties. Residential relocation and displacement of drug markets were reported as beneficial for persons in contemplative and later stages of recovery. Conclusion These findings support a holistic approach to urban redevelopment that increases access to employment opportunities and affordable housing and ensures equitable coverage of public services such as law enforcement. PMID:23647924

  3. Educational Policy and Rural Re-development in Post-industrial Society: The Case of the United States of America.

    ERIC Educational Resources Information Center

    Slocum, Walter L.

    Recent emphasis on industrial and urban development is described as a factor contributing to the greatly accelerated flight from the land. The concept of rural redevelopment is examined along with educational policy changes needed for rural redevelopment. The author's stated opinion is that rural redevelopment in the United States and other…

  4. Brownfields initiatives offer few incentives for prospective developers, purchasers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wesolowski, T.; Antol, S.M.

    There has been much discussion and analysis in recent years among government agencies and state legislators regarding redevelopment of contaminated industrial sites, or brownfields. The goal of brownfields program is to encourage developers and purchasers to redevelop existing industrial sites, rather than choosing greenfield suburban sites. These programs create initiatives for redevelopment, such as reducing the potential liability of innocent purchasers or developers, streamlining administrative process relating to cleanups, and issuing guidance on cleanup methods and standards. Although such attempts are admirable and important, brownfields initiatives have failed because they address only one component of what is necessary to achievemore » redevelopment. The initiatives attempt to minimize disincentives to brownfields redevelopment; however, what is necessary for progress in this area is to create real economic and other incentives to make such redevelopment more attractive.« less

  5. Superfund Redevelopment Initiative Quarterly Webinar Series

    EPA Pesticide Factsheets

    Superfund Redevelopment Initiative hosts a series of quarterly webinars on redevelopment of Superfund sites across the country that discuss the different types of reuse that are possible and share how particular sites have become reuse success stories.

  6. Socioeconomic Impact Analysis Study. Disposal and Reuse of Castle Air Force Base, California

    DTIC Science & Technology

    1994-01-01

    and redevelopment agencies in the development of their reuse plan. The scope of this study includes economic activity, population, housing, public...Aviation Alternative include industrial (agricultural related research and development [991 acres]), institutional (educational [545 acres]), and public...would vary with the reuse alternative developed . The net effects are the total reuse-related direct and secondary employment and population decreased

  7. BROWNFIELDS REDEVELOPMENT CAN BENEFIT FROM USING RISK ASSESSMENT PRINCIPLES

    EPA Science Inventory

    Brownfields are defined by the USEPA a "abandoned, idled, or under-used industrial and commercial facilities where expansion or redevelopment is complicated by real or perceived environmental contamination." The costs associated with the lack of Brownfields redevelopment include ...

  8. Environmental Assessment for Vandenberg Gate Complex Construction, Dorm Construction and Demolition at Hanscom Air Force Base, Massachusetts

    DTIC Science & Technology

    2014-12-01

    MA January 2015 3 There are no existing underground stormwater drains in the area of the new Vandenberg Gate Complex. The addition of... stormwater management systems that utilize the pervious landscape, vegetative filtration, sediment removal, infiltration via bioswales, deep sump...Airmen Dormitory construction, a base-wide stormwater standard requires redevelopment projects to reduce stormwater rate and volume by 10% over the

  9. RISK ASSESSMENT/RISK MANAGEMENT AND COMMUNICATION FOR BROWNFIELDS REDEVELOPMENT DECISION MAKING

    EPA Science Inventory

    Brownfields redevelopment is an integrated process which involves individuals from varied disciplines and with widely varied interest in the project. it involves a group of developers, their financial backers, the local governments who permit the redevelopment of the site or site...

  10. A Spatially Detailed Model of Isometric Contraction Based on Competitive Binding of Troponin I Explains Cooperative Interactions between Tropomyosin and Crossbridges

    PubMed Central

    Land, Sander; Niederer, Steven A.

    2015-01-01

    Biophysical models of cardiac tension development provide a succinct representation of our understanding of force generation in the heart. The link between protein kinetics and interactions that gives rise to high cooperativity is not yet fully explained from experiments or previous biophysical models. We propose a biophysical ODE-based representation of cross-bridge (XB), tropomyosin and troponin within a contractile regulatory unit (RU) to investigate the mechanisms behind cooperative activation, as well as the role of cooperativity in dynamic tension generation across different species. The model includes cooperative interactions between regulatory units (RU-RU), between crossbridges (XB-XB), as well more complex interactions between crossbridges and regulatory units (XB-RU interactions). For the steady-state force-calcium relationship, our framework predicts that: (1) XB-RU effects are key in shifting the half-activation value of the force-calcium relationship towards lower [Ca2+], but have only small effects on cooperativity. (2) XB-XB effects approximately double the duty ratio of myosin, but do not significantly affect cooperativity. (3) RU-RU effects derived from the long-range action of tropomyosin are a major factor in cooperative activation, with each additional unblocked RU increasing the rate of additional RU’s unblocking. (4) Myosin affinity for short (1–4 RU) unblocked stretches of actin of is very low, and the resulting suppression of force at low [Ca2+] is a major contributor in the biphasic force-calcium relationship. We also reproduce isometric tension development across mouse, rat and human at physiological temperature and pacing rate, and conclude that species differences require only changes in myosin affinity and troponin I/troponin C affinity. Furthermore, we show that the calcium dependence of the rate of tension redevelopment ktr is explained by transient blocking of RU’s by a temporary decrease in XB-RU effects. PMID:26262582

  11. Assessing the effect of publicly assisted brownfield redevelopment on surrounding property values

    Treesearch

    Christopher A. De Sousa; Changson Wu; Lynne M. Westphal

    2009-01-01

    This study measures and compares the impact of publicly assisted brownfield redevelopment on nearby residential property values in Milwaukee and Minneapolis. It also examines the influence of land use, neighborhood characteristics, and other redevelopment factors on this impact. The research approach incorporates a hedonic method to quantify nearby property value...

  12. Surface and subsurface geologic risk factors to ground water affecting brownfield redevelopment potential.

    PubMed

    Kaufman, Martin M; Murray, Kent S; Rogers, Daniel T

    2003-01-01

    A model is created for assessing the redevelopment potential of brownfields. The model is derived from a space and time conceptual framework that identifies and measures the surface and subsurface risk factors present at brownfield sites. The model then combines these factors with a contamination extent multiplier at each site to create an index of redevelopment potential. Results from the application of the model within an urbanized watershed demonstrate clear differences between the redevelopment potential present within five different near-surface geologic units, with those units containing clay being less vulnerable to subsurface contamination. With and without the extent multiplier, the total risk present at the brownfield sites within all the geologic units is also strongly correlated to the actual costs of remediation. Thus, computing the total surface and subsurface risk within a watershed can help guide the remediation efforts at broad geographic scales, and prioritize the locations for redevelopment.

  13. Base Realignments and Closures: Report of the Defense Secretary’s Commission

    DTIC Science & Technology

    1988-12-29

    recommended to the Secretary of Defense several actions that should be taken to aid When it was determined that an local communities in their redevelopment...approved plans. Unlike some previous In many cases, they brought to the task a reviews, the Commission’s approach , first-hand knowledge of military...the by a professional staff (see Appendix D). individual Services. For example, the Air Force, driven by severe current and In organizing to accomplish

  14. Brownfields Redevelopment, Preferences and Public Involvement: A Case Study of an Ethnically Mixed Neighborhood.

    ERIC Educational Resources Information Center

    Greenberg, Michael; Lewis, M. Jane

    2000-01-01

    A survey of over 200 residents of a largely Hispanic census tract in Perth Amboy, NJ found that residents preferred recreation, cultural, and other community facilities be the aim of brownfield re-development. They were less interested in industry and business, the promoted aim of such re-development. Three-quarters of respondents indicated a…

  15. Espionage Threats at Federal Laboratories: Balancing Scientific Cooperation While Protecting Critical Information

    DTIC Science & Technology

    2013-05-16

    major new institutes 1n neuroscience and genomic medicine; and redeveloped much of its campus. In 2007, Dr. Vest was elected to serve as president of...which is a hearing unto itself— ‘‘the largest transfer of wealth in history’’ as the Director of NSA has called cyber attacks against us. So when...Navy 47 Army 43 Govt. Contractor 42 Air Force - 23 CIA -15 State _.. 12 FBI .8 NSA .5 University Employee ~6 Marines 1114 DIA 1114 ~ DoD

  16. Masticatory myosin unveiled: first determination of contractile parameters of muscle fibers from carnivore jaw muscles.

    PubMed

    Toniolo, Luana; Cancellara, Pasqua; Maccatrozzo, Lisa; Patruno, Marco; Mascarello, Francesco; Reggiani, Carlo

    2008-12-01

    Masticatory myosin heavy chain (M MyHC) is a myosin subunit isoform with expression restricted to muscles derived from the first branchial arch, such as jaw-closer muscles, with pronounced interspecies variability. Only sparse information is available on the contractile properties of muscle fibers expressing M MyHC (M fibers). In this study, we characterized M fibers isolated from the jaw-closer muscles (temporalis and masseter) of two species of domestic carnivores, the cat and the dog, compared with fibers expressing slow or fast (2A, 2X, and 2B) isoforms. In each fiber, during maximally calcium-activated contractions at 12 degrees C, we determined isometric-specific tension (P(o)), unloaded shortening velocity (v(o)) with the slack test protocol, and the rate constant of tension redevelopment (K(TR)) after a fast shortening-relengthening cycle. At the end of the mechanical experiment, we identified MyHC isoform composition of each fiber with gel electrophoresis. Electrophoretic migration rate of M MyHC was similar in both species. We found that in both species the kinetic parameters v(o) and K(TR) of M fibers were similar to those of 2A fibers, whereas P(o) values were significantly greater than in any other fiber types. The similarity between 2A and M fibers and the greater tension development of M fibers were confirmed also in mechanical experiments performed at 24 degrees C. Myosin concentration was determined in single fibers and found not different in M fibers compared with slow and fast fibers, suggesting that the higher tension developed by M fibers does not find an explanation in a greater number of force generators. The specific mechanical characteristics of M fibers might be attributed to a diversity in cross-bridge kinetics.

  17. Cross-border cooperation potential in fostering redevelopment of degraded border areas - a case study approach.

    NASA Astrophysics Data System (ADS)

    Alexandre Castanho, Rui; Ramírez, Beatriz; Loures, Luis; Fernández-Pozo, Luis; Cabezas, José

    2017-04-01

    Border interactions have reached unprecedented levels in recent decades, not only due to their potential for territorial integration but also considering their role in supranational processes, such as landscape reclamation, infrastructure development and land use planning on European territory. In this scenario, successful examples related to the redevelopment of degraded areas have been showing positive impacts at several levels, such as the social, economic, environmental and aesthetic ones which have ultimately related this process, positively, to sustainability issues. However, concerning to border areas, and due to their inherent legislative and bureaucratic conflicts, the intervention in these areas is more complex. Still, and taking into account previously developed projects and strategies of cross-border cooperation (CBC) in European territory it is possible to identified that the definition of common master plans and common objectives are critical issues to achieve the desired territorial success. Additionally, recent studies have put forward some noteworthy ideas highlighting that it is possible to establish a positive correlation between CBC processes and an increasing redevelopment of degraded border areas, with special focus on the reclamation of derelict landscapes fostering soil reuse and redevelopment. The present research, throughout case study analysis at the Mediterranean level - considering case studies from Portugal, Spain, Monaco and Italy - which presents specific data on border landscape redevelopment, enables us to conclude that CBC processes have a positive influence on the potential redevelopment of degraded border areas, considering not only urban but also rural land. Furthermore, this paper presents data obtained through a public participation process which highlights that these areas present a greater potential for landscape reclamation, fostering resource sustainability and sustainable growth. Keywords: Spatial planning; Land degradation; Redevelopment; Cross-border cooperation (CBC); Land use.

  18. Redevelopment Ready Communities: Best Practices and Scoring System

    EPA Pesticide Factsheets

    This report discusses the Redevelopment Ready Communities program in southeast Michigan, which encourages mature suburbs to bolster their competitiveness by making development more efficient and less complicated.

  19. Environmental Assessment. Moanalua Shopping Center Redevelopment Oahu, Hawaii

    DTIC Science & Technology

    2004-08-01

    redevelop the approximately 15-acre MSC site as in-kind consideration for the fair market rent which would be due under a 40-year lease of the...the existing approximately 15-acre (6-hectare) MSC site as in-kind consideration for the fair market rent which would be due under a 40-year lease of...would redevelop the existing MSC site as in-kind consideration for the fair market rent which would be due under a 40-year lease of the properties

  20. Downtown Redevelopment of Former Gas Station in Millen, GA

    EPA Pesticide Factsheets

    From the 1940s through the late 1970s, a former SOC Gasoline Station and repair shop was active on a prominent corner of Downtown Millen, immediately across the street from the Historic County Courthouse. The City of Millen was able to use their 2006 assessment grant to conduct Phase I and II due diligence activities prior to taking ownership of the site. The City of Millen successfully took on the role of redeveloper and actively sought cleanup and redevelopment funding.

  1. Postcrisis redevelopment of sustainable healthcare systems.

    PubMed

    Kanadanian, Koren V; Haan, Constance K

    2014-01-01

    Research and field experience have identified a global gap in postdisaster rebuilding of healthcare systems due to the current primary focus on returning devastated community infrastructures to predisaster conditions. Disasters, natural or man-made, present an opportunity for communities to rebuild, restructure, and redefine their predisaster states, creating more resilient and sustainable healthcare systems. A model for sustainable postdisaster healthcare rebuilding was developed by bridging identified gaps in the literature on the processes of developing healthcare systems postdisaster and utilizing evidence from the literature on postdisaster community reconstruction. The proposed model-the Sustainable Healthcare Redevelopment Model-is designed to guide communities through the process of recovery, and identifies four stages for rebuilding healthcare systems: (1) response, (2) recovery, (3) redevelopment, and (4) sustainable development. Implementing sustainable healthcare redevelopment involves a bottom-up approach, where community stakeholders have the ability to influence policy decisions. Relationships within internal government agencies and with public-private partnerships are necessary for successful recovery. The Sustainable Healthcare Redevelopment Model can serve as a guideline for delivery of healthcare services following disaster or conflict and use of crisis as a window of opportunity to improve the healthcare delivery system and incorporate resilience into the healthcare infrastructure.

  2. Barre City, Vermont Accounts for Climate Change within a Brownfield Redevelopment Plan

    EPA Pesticide Factsheets

    The Summer Street Housing Limited Partnership, a partnership between the Central Vermont Community Land Trust (CVCLT) and the non-profit Housing Vermont, sought to redevelop a brownfields* site in Barre, Vermont.

  3. Energy Sources and Systems Analysis: 40 South Lincoln Redevelopment District (Full Report)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    2011-08-01

    This report presents the results of a case study to analyze district energy systems for their potential use in a project that involves redeveloping 270 units of existing public housing, along with other nearby sites. When complete, the redevelopment project will encompass more than 900 mixed-income residential units, commercial and retail properties, and open space. The analysis estimated the hourly heating, cooling, domestic hot water, and electric loads required by the community; investigated potential district system technologies to meet those needs; and researched available fuel sources to power such systems.

  4. Measuring site-level success in brownfield redevelopments: a focus on sustainability and green building.

    PubMed

    Wedding, G Christopher; Crawford-Brown, Douglas

    2007-10-01

    This research has met the following four objectives within the broader research topic of characterizing and quantifying success in brownfield revitalization: (1) to define 40 total indicators that define and determine the success of brownfield redevelopments in four categories: environment-health, finance, livability, and social-economic; (2) to use these indicators to develop a partially automated tool that stakeholders in brownfield redevelopment may use to more easily assess and communicate success (or failures) in these projects; (3) to integrate "green" building as an important aspect of successful brownfield redevelopments; and (4) to develop this tool within the framework of a specific multi-attribute decision method (MADM), the analytical hierarchical process (AHP). Future research should include the operationalization and application of this tool to specific sites. Currently, no such indicator framework or automated tool is known to exist or be in use. Indicators were chosen because of their ability to reduce data into comprehensible measurements and to systematically measure success in a standardized fashion. Appropriate indicators were selected based on (1) interviews with prominent private developers and national leaders in brownfield redevelopment, (2) a review of the relevant literature, (3) objective hierarchies created in this project, and (4) the ability for each indicator to serve goals in more than one of the four categories described above. These were combined to form the Sustainable Brownfields Redevelopment (SBR) Tool. A survey was conducted to serve as a preliminary assessment and proposed methodology for judging the validity of the SBR Tool. Professionals in the academic, private, and public sector were asked to provide an evaluation of the management tool and a weighting of the relative importance of each indicator and each of the four categories listed previously. Experts rated the tool at 7.68 out of 10 suggesting that this framework will be useful in evaluating these redevelopments upon completion and in formulating initial site plans and building design.

  5. Reconsidering brownfield redevelopment strategy in China's old industrial zone: a health risk assessment of heavy metal contamination.

    PubMed

    Ren, Wanxia; Geng, Yong; Ma, Zhixiao; Sun, Lina; Xue, Bing; Fujita, Tsuyoshi

    2015-02-01

    Urban environmental quality in brownfield redevelopment sites is of vital importance after most of former industrial areas were replanned or changed into residential and recreational areas. Hence, it is necessary to rethink if those brownfield redevelopment sites have been cleaned up so that there will be no negative health impacts to local residents. Under such a circumstance, this paper aims to evaluate the contamination level of heavy metals within a brownfield redevelopment site in China, namely, the Tiexi old industrial zone in Shenyang. Surface soil and dust samples were collected from local industrial sites, residential/commercial sites, traffic sites, and recreational sites, respectively. Our analysis results revealed that although the soils in the brownfield redevelopment sites had been treated and remediated, heavy metal pollution still exists in certain sites, especially in the current industrial sites that will be planned into residential/commercial or recreational zones, and the current residential sites where the former industrial sites located, showing that past industrial activities did and will continue to influence the soil quality. Further health risk assessment indicates that As and Pb generated from industrial sites and traffic sites has a potential to pose serious health risks to local residents, especially children. The hotspots with more serious health risks to children are mainly concentrated in the areas close to the former Shenyang Smelting Plant. After one decade of redevelopment, the Tiexi old industrial zone has become a new urban area which is not suitable for large-scaled soil remediation efforts. Thus, the phytoremediation by trees or herbs in heavy-metal-contaminated land is more appropriate and should be embedded into urban green land planning. This study provides innovative policy insights on urban brownfield redevelopment to both governmental officials and related stakeholders so that they can make appropriate remediation actions.

  6. Air and Water Quality Impacts of Brownfields Redevelopment: A Study of Five Communities

    EPA Pesticide Factsheets

    This Study of Five Communities tests an analytical approach to quantifying the environmental impacts of multiple redevelopment projects in a given municipal area in a manner that can be replicated in other regions.

  7. Hazardous Waste Cleanup: Tecumseh Redevelopment Incorporated in Lackawanna, New York

    EPA Pesticide Factsheets

    Tecumseh Redevelopment Inc. (Tecumseh) owns approximately 1,100 acres of property located along NYS Route 5, adjacent to Lake Erie, in Lackawanna. The property comprises a significant portion of the former Bethlehem Steel Corporation’s (BSC) Lackawanna

  8. SWMM 5 REDEVELOPMENT QUALITY ASSURANCE PROGRAM

    EPA Science Inventory

    EPA recently released a new version of the Storm Water Management Model (SWMM) that combines a new interface with a completely re-written computational engine. The SWMM redevelopment project proceeded under a Quality Assurance Project Plan (QAPP) that describes methods and proced...

  9. Sites in Mass. and Conn. are on EPA's Superfund Redevelopment Focus List

    EPA Pesticide Factsheets

    Today, the US EPA released its initial list of Superfund National Priorities List sites with the greatest expected redevelopment & commercial potential including two sites in New England: New Bedford Harbor in New Bedford, Mass. & Raymark Industries Inc.

  10. SMART GUIDANCE AND SMARTE - TOOLS FOR DEVELOPING SITE SPECIFIC REDEVELOPMENT PLANS

    EPA Science Inventory

    Site-specific Management Approaches and Redevelopment Tools (SMART) Guidance and its electronic counterpart, SMARTe are being developed jointly with the German Federal Ministry of Education and Research and the Interstate Technology Regulatory Council. These products will assist ...

  11. The Entrepreneurial Approach to Participation in Community Redevelopment Activities.

    ERIC Educational Resources Information Center

    Wickert, Donald M.

    1985-01-01

    Discusses the use of tax increment financing to fund public redevelopment or urban rehabilitation projects and the opportunity for school districts to take advantage of such projects to supplement reduced property tax revenues in areas of declining valuation. (PGD)

  12. TECHNICAL APPROACHES TO CHARACTERIZING AND REDEVELOPING BROWNFIELDS SITES: MUNICIPAL LANDFILLS AND ILLEGAL DUMPS

    EPA Science Inventory

    The guidance document gives assistance to communities, decision-makers, states and municipalities, academia, and the private sector to address issues related to the redevelopment of Brownfields sites, specifically, municipal landfill and illegal dump sites. The document helps use...

  13. Equitable Redevelopment of Petroleum Brownfields for Zuni Pueblo and Other Tribal Communities

    EPA Pesticide Factsheets

    EPA worked with the Zuni Environmental Protection Program to develop a step-by-step guide to redeveloping petroleum brownfields sites. The guide focuses on three sites on Zuni Pueblo in New Mexico but provides information useful to tribes nationwide.

  14. In-service evaluation of major urban arterials with landscaped medians : phase III.

    DOT National Transportation Integrated Search

    2013-06-01

    Several cities have implemented redevelopment plans that include the re-design of major regional arterials in order to raise the quality of life of those living, working, and shopping along, or near the arterial. Many of these redevelopment efforts i...

  15. Integrating Local Green Assets into Brownfields Redevelopment: Tools and Examples

    EPA Science Inventory

    EnviroAtlas is a free, online public mapping tool that characterizes green infrastructure and its connection to human health and wellness. The high resolution data contained in this tool can be used to incorporate local green infrastructure into Brownfields redevelopment to benef...

  16. Environmental Impact Statement. Disposal and Reuse of Wurtsmith Air Force Base, Michigan

    DTIC Science & Technology

    1993-09-01

    Mephitis mephitis Meadow vole Microtus pennsy/vanicus House mouse Mus musculus Long-tailed weasel Mustela frenata Mink Mustela vison Little brown bat...FB Disposal and Reuse FEIS S-7 󈧰 *0 0.00 .4- - a a~ SO Sig > U 00 - 00 .C -* LW5 0C~ 7 cca!’ ae- 2 L a a Co’s mU E ’ED IA ~ EU a a c I- .. 9U2= 4 0...and utility effects of each proposal as a function of proposed land use and employment due to redevelopment, and anticipated phasing of the various

  17. 76 FR 78938 - Carpinteria Offshore Field Redevelopment Project-Developmental Drilling Into the Carpinteria...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... DEPARTMENT OF THE INTERIOR Bureau of Ocean Energy Management Carpinteria Offshore Field Redevelopment Project--Developmental Drilling Into the Carpinteria Offshore Field Oil and Gas Reserves... Lands Commission (CSLC) intend to jointly review a proposal to develop offshore oil and gas resources...

  18. 24 CFR 599.107 - Required State and local commitments.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the Renewal Community, such as: (1) Density bonus. Permission to develop or redevelop real property at... or other real property-related incentive for the development, redevelopment, or preservation of a... gift or sale at below fair market value of surplus real property held by State or local governments...

  19. 24 CFR 599.107 - Required State and local commitments.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... the Renewal Community, such as: (1) Density bonus. Permission to develop or redevelop real property at... or other real property-related incentive for the development, redevelopment, or preservation of a... gift or sale at below fair market value of surplus real property held by State or local governments...

  20. 24 CFR 599.107 - Required State and local commitments.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the Renewal Community, such as: (1) Density bonus. Permission to develop or redevelop real property at... or other real property-related incentive for the development, redevelopment, or preservation of a... gift or sale at below fair market value of surplus real property held by State or local governments...

  1. 24 CFR 599.107 - Required State and local commitments.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... the Renewal Community, such as: (1) Density bonus. Permission to develop or redevelop real property at... or other real property-related incentive for the development, redevelopment, or preservation of a... gift or sale at below fair market value of surplus real property held by State or local governments...

  2. 24 CFR 599.107 - Required State and local commitments.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the Renewal Community, such as: (1) Density bonus. Permission to develop or redevelop real property at... or other real property-related incentive for the development, redevelopment, or preservation of a... gift or sale at below fair market value of surplus real property held by State or local governments...

  3. Energy Sources and Systems Analysis: 40 South Lincoln Redevelopment District (Short Report)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    2011-08-01

    This report presents the a brief overview of the results of a case study to analyze district energy systems for their potential use in a project that involves redeveloping 270 units of existing public housing, along with other nearby sites. When complete, the redevelopment project will encompass more than 900 mixed-income residential units, commercial and retail properties, and open space. The analysis estimated the hourly heating, cooling, domestic hot water, and electric loads required by the community; investigated potential district system technologies to meet those needs; and researched available fuel sources to power such systems. A full report of thismore » case study is also available.« less

  4. Validation and Application of the ReaxFF Reactive Force Field to Hydrocarbon Oxidation Kinetics

    DTIC Science & Technology

    2016-06-23

    AFRL-AFOSR-VA-TR-2016-0278 Validation and application of the ReaxFF reactive force field to hydrocarbon oxidation kinetics Adrianus Van Duin...application of the ReaxFF reactive force field to hydrocarbon oxidation kinetics 5a.  CONTRACT NUMBER 5b.  GRANT NUMBER FA9550-14-1-0355 5c.  PROGRAM...Chenoweth Dec14 Validation and application of the ReaxFF reactive force field to hydrocarbon oxidation kinetics DISTRIBUTION A: Distribution approved for

  5. Calm Active and Focused: Children's Responses to an Organic Outdoor Learning Environment

    ERIC Educational Resources Information Center

    Nedovic, Sonya; Morrissey, Anne-Marie

    2013-01-01

    This study reports on children's observed responses to natural features introduced in the redevelopment of a childcare centre garden. Using an action research approach, the redevelopment was based on the preferences of the director, staff and 18 three- to four-year-olds, as expressed through interviews, conversations, photographs and drawings.…

  6. UNITED STATES AND GERMAN BILATERAL WORKING GROUP ON REDEVELOPMENT OF POTENTIALLY CONTAMINATED SITES SITE-SPECIFIC MANAGEMENT APPROACHES AND REDEVELOPMENT TOOLS - RESOURCE (SMARTRESOURCE)

    EPA Science Inventory

    The United States Environmental Protection Agency (EPA) and the German Federal Ministry of Education and Research (BMBF) continues an ongoing partnership to gain an understanding of each other's approach to the cleanup of chemical contamination in order to protect human health ...

  7. Brownfields and health risks--air dispersion modeling and health risk assessment at landfill redevelopment sites.

    PubMed

    Ofungwu, Joseph; Eget, Steven

    2006-07-01

    Redevelopment of landfill sites in the New Jersey-New York metropolitan area for recreational (golf courses), commercial, and even residential purposes seems to be gaining acceptance among municipal planners and developers. Landfill gas generation, which includes methane and potentially toxic nonmethane compounds usually continues long after closure of the landfill exercise phase. It is therefore prudent to evaluate potential health risks associated with exposure to gas emissions before redevelopment of the landfill sites as recreational, commercial, and, especially, residential properties. Unacceptably high health risks would call for risk management measures such as limiting the development to commercial/recreational rather than residential uses, stringent gas control mechanisms, interior air filtration, etc. A methodology is presented for applying existing models to estimate residual landfill hazardous compounds emissions and to quantify associated health risks. Besides the toxic gas constituents of landfill emissions, other risk-related issues concerning buried waste, landfill leachate, and explosive gases were qualitatively evaluated. Five contiguously located landfill sites in New Jersey intended for residential and recreational redevelopment were used to exemplify the approach.

  8. Stormwater Infrastructure at Risk: Predicting the Impacts of Increased Imperviousness due to Infill Development in a Semi-arid Urban Neighborhood

    NASA Astrophysics Data System (ADS)

    Hogue, T. S.; Panos, C.; McCray, J. E.; Gilliom, R.

    2017-12-01

    This research investigates the impacts of infill development (or "redevelopment") on urban stormwater runoff and explores avenues for re-inventing stormwater management strategies for the City of Denver, Colorado. As a rapidly developing city, Denver is facing a cycle of increasing population and redevelopment in the form of infill (where under-utilized parcels within the City are redeveloped into high-density residential land uses). Infill development increases stormwater runoff by introducing more impervious surfaces, including roofs and driveways, which produce more runoff (additional stormwater). However, there is debate on the impact of infill patterns on runoff behavior, peak flows, and flood frequency events. We used a calibrated, high-resolution PCSWMM model to simulate three redevelopment scenarios within the 1000-acre Berkeley neighborhood of northwest Denver. The scenarios utilized future predictions of redevelopment to simulate increases in imperviousness by 1.1, 4.5, and 8.7 percent by 2024, 2034, and 2044, respectively, for a range of design storms. Results predict that, on average, for each 1% increase in impervious area due to infill development, surface runoff volume will increase by 1.28% in the Berkeley neighborhood. Results demonstrate the limitations of the existing storm sewer network as pipes throughout the catchment reach capacity for events larger than the 2-yr storm for all three scenarios. Spatial maps of the catchment pinpoint subcatchments and sewer nodes of concern, namely surrounding a rapidly growing business corridor and the local Interstate. Overall, results indicate the infrastructure of the Berkeley neighborhood may be at risk, and that current stormwater capture policies may need to be revisited to accommodate both future infill development and climate change. This research provides a quantitative basis for implementing potential changes as well as examining the possibility of using the additional stormwater from redevelopment for beneficial use within Denver.

  9. 26 CFR 1.144-1 - Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 2 2012-04-01 2012-04-01 false Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds. 1.144-1 Section 1.144-1 Internal Revenue INTERNAL...) Tax Exemption Requirements for State and Local Bonds § 1.144-1 Qualified small issue bonds, qualified...

  10. 26 CFR 1.144-1 - Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 2 2013-04-01 2013-04-01 false Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds. 1.144-1 Section 1.144-1 Internal Revenue INTERNAL...) Tax Exemption Requirements for State and Local Bonds § 1.144-1 Qualified small issue bonds, qualified...

  11. 26 CFR 1.144-1 - Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds. 1.144-1 Section 1.144-1 Internal Revenue INTERNAL...) Tax Exemption Requirements for State and Local Bonds § 1.144-1 Qualified small issue bonds, qualified...

  12. 26 CFR 1.144-1 - Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 2 2014-04-01 2014-04-01 false Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds. 1.144-1 Section 1.144-1 Internal Revenue INTERNAL...) Tax Exemption Requirements for State and Local Bonds § 1.144-1 Qualified small issue bonds, qualified...

  13. 26 CFR 1.144-1 - Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 2 2011-04-01 2011-04-01 false Qualified small issue bonds, qualified student loan bonds, and qualified redevelopment bonds. 1.144-1 Section 1.144-1 Internal Revenue INTERNAL...) Tax Exemption Requirements for State and Local Bonds § 1.144-1 Qualified small issue bonds, qualified...

  14. Environmental liability and redevelopment of old industrial land.

    PubMed

    Sigman, Hilary

    2010-01-01

    Many communities are concerned about the reuse of potentially contaminated land (brownfields) and believe that environmental liability is a hindrance to redevelopment. However, with land price adjustments, liability might not impede the reuse of this land. This article studies state liability rules-specifically, strict liability and joint and several liability-that affect the level and distribution of expected costs of private cleanup. It explores the effects of this variation on industrial land prices and vacancy rates and on reported brownfields in a panel of cities across the United States. In the estimated equations, joint and several liability reduces land prices and increases vacancy rates in central cities. The results suggest that liability is at least partly capitalized but does still deter redevelopment.

  15. Sustainability Criteria for Planning, Constructing, and Operating Contingency Bases

    DTIC Science & Technology

    2012-05-22

    Planning 14 and airfields. Don’t want to clean up a site to use i t. Too risky, takes CREDIT 2 Brownfield Redevelopment no Planning N/A too long. Base...clean up a site to use i t. Too risky, takes CREDIT 2 Brownfield Redevelopment no Planning N/A too long. Base camp site location is chosen based on

  16. RCRA, Superfund and EPCRA hotline training module. Introduction to: Brownfields economic redevelopment initiative and environmental justice (updated february 1998)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    Topics addressed include the following: Authority for the Brownfields Initiative; The Brownfields Economic Redevelopment Initiative; (Brownfields Assessment Demonstration Pilots; Brownfields Revolving Loan Funds Pilots; Brownfields National Partnership Action Agenda; Brownfields Showcase Communities; Clarification of Liability Issues; Partnership and Outreach; Job Development and Training; and Brownfields Tax Incentive); Environmental Justice; and Summary.

  17. An Overview of Manpower Development and Training under Redevelopment Area Residents Program, 1963-1966.

    ERIC Educational Resources Information Center

    Division of Manpower Development and Training. , BAVT.

    Information regarding programs under Section 241 of the Manpower Development and Training Act which provides training for redevelopment area residents is presented. Appropriations increased from $6,500,000 in 1965 to $24,000,000 in 1967. Training costs declined in 1964 and 1965 compared to 1963 but were doubled in 1966 because of an extension of…

  18. Integration of the subsurface and the surface sectors for a more holistic approach for sustainable redevelopment of urban brownfields.

    PubMed

    Norrman, Jenny; Volchko, Yevheniya; Hooimeijer, Fransje; Maring, Linda; Kain, Jaan-Henrik; Bardos, Paul; Broekx, Steven; Beames, Alistair; Rosén, Lars

    2016-09-01

    This paper presents a holistic approach to sustainable urban brownfield redevelopment where specific focus is put on the integration of a multitude of subsurface qualities in the early phases of the urban redevelopment process, i.e. in the initiative and plan phases. Achieving sustainability in brownfield redevelopment projects may be constrained by a failure of engagement between two key expert constituencies: urban planners/designers and subsurface engineers, leading to missed opportunities and unintended outcomes in the plan realisation phase. A more integrated approach delivers greater benefits. Three case studies in the Netherlands, Belgium and Sweden were used to test different sustainability assessment instruments in terms of the possibility for knowledge exchange between the subsurface and the surface sectors and in terms of cooperative learning among experts and stakeholders. Based on the lessons learned from the case studies, a generic decision process framework is suggested that supports holistic decision making. The suggested framework focuses on stakeholder involvement, communication, knowledge exchange and learning and provides an inventory of instruments that can support these processes. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Characterization of High-Frequency Excitation of a Wake by Simulation

    NASA Technical Reports Server (NTRS)

    Cain, Alan B.; Rogers, Michael M.; Kibens, Valdis; Mansour, Nagi (Technical Monitor)

    2003-01-01

    Insights into the effects of high-frequency forcing on free shear layer evolution are gained through analysis of several direct numerical simulations. High-frequency forcing of a fully turbulent plane wake results in only a weak transient effect. On the other hand, significant changes in the developed turbulent state may result when high-frequency forcing is applied to a transitional wake. The impacts of varying the characteristics of the high-frequency forcing are examined, particularly, the streamwise wavenumber band in which forcing is applied and the initial amplitude of the forcing. The high-frequency excitation is found to increase the dissipation rate of turbulent kinetic energy, to reduce the turbulent kinetic energy production rate, and to reduce the turbulent kinetic energy suppression increases with forcing amplitude once a threshold level has been reached. For a given initial forcing energy, the largest reduction in turbulent kinetic energy density was achieved by forcing wavenumbers that are about two to three times the neutral wavenumber determined from linear stability theory.

  20. Report on 2005 Defense Base Closure and Realignment Implementation

    DTIC Science & Technology

    2011-09-01

    been agreed to by the redevelopment authority? Yes Description of redevelopment plan: Education Conveyance to Texarkana College. Quantity of...1993 1998 2008 1995 2014 2014 2002 2016 2010 2011 2012 20252013 2014 2015 2016 2017 2018 2019 2020 2021 2022 2024...1996 2001 2008 2008 1990 2008 2008 2008 2010 2011 2012 20252013 2014 2015 2016 2017 2018 2019 2020 2021 2022 2024

  1. Understanding Long-term Greenness, Water Use, and Redevelopment in Denver, Colorado

    NASA Astrophysics Data System (ADS)

    Neel, A.; Hogue, T. S.; Read, L.

    2016-12-01

    In 2015 the U.S. Census Bureau's found Denver to have the fastest growth rate among large cities in America. With the population of Metro Denver expected to increase from 2.9 to 3.3 million it is critical to consider the impacts of expected redevelopment and increased housing density on the City's ecosystem and future water supply. While prior studies have shown outdoor water use to account for as much as 40-60% of single-family residential water use in western cities, currently no published research examines patterns in urban vegetation, greenness, temperature and water use for cities in the Rocky Mountain West. Normalized Differential Vegetation Index (NDVI) calculated from Landsat imagery was examined to assess how redevelopment in Denver's urban center impacts regional greenness patterns, land surface temperatures and water budgets. Over the last twenty-seven years Denver has shown an overall 4.4% decrease in greenness, with a more rapid decline starting in 2006. While NDVI and cumulative precipitation have a significant relationship over the study period, decreasing NDVI trends across all seasons suggests other factors, such as redevelopment, may be influencing the city's greenness. Comparing water use, NDVI, and precipitation reveals that not only do climate and redevelopment affect NDVI patterns, but mandated water restrictions may also be having a significant impact on NDVI values. NDVI and precipitation patterns are being assessed against regional surface temperatures over time. Surface temperatures, taken from Landsat data, reveal that Urban Heat Island effect may become more pronounced with decreasing NDVI values. As Denver continues to grow, managers can utilize results to better inform decisions about landscape patterns relative to outdoor water use, the effectiveness of restrictions on consumption, and future planning for green infrastructure.

  2. Using the Community Readiness Model to Examine the Built and Social Environment: A Case Study of the High Point Neighborhood, Seattle, Washington, 2000–2010

    PubMed Central

    Sharify, Denise Tung; Blake, Bonita; Phillips, Tom; Whitten, Kathleen

    2014-01-01

    Background Residents of many cities lack affordable, quality housing. Economically disadvantaged neighborhoods often have high rates of poverty and crime, few institutions that enhance the quality of its residents’ lives, and unsafe environments for walking and other physical activity. Deteriorating housing contributes to asthma-related illness. We describe the redevelopment of High Point, a West Seattle neighborhood, to improve its built environment, increase neighborhood physical activity, and reduce indoor asthma triggers. Community Context High Point is one of Seattle’s most demographically diverse neighborhoods. Prior to redevelopment, it had a distressed infrastructure, rising crime rates, and indoor environments that increased asthma-related illness in children and adolescents. High Point residents and partners developed and implemented a comprehensive redevelopment plan to create a sustainable built environment to increase outdoor physical activity and improve indoor environments. Methods We conducted a retrospective analysis of the High Point redevelopment, organized by the different stages of change in the Community Readiness Model. We also examined the multisector partnerships among government and community groups that contributed to the success of the High Point project. Outcome Overall quality of life for residents improved as a result of neighborhood redevelopment. Physical activity increased, residents reported fewer days of poor physical or mental health, and social connectedness between neighbors grew. Asthma-friendly homes significantly decreased asthma-related illness among children and adolescents. Interpretation Providing affordable, quality housing to low-income families improved individual and neighborhood quality of life. Efforts to create social change and improve the health outcomes for entire populations are more effective when multiple organizations work together to improve neighborhood health. PMID:25376016

  3. Using the community readiness model to examine the built and social environment: a case study of the High Point neighborhood, Seattle, Washington, 2000-2010.

    PubMed

    Buckner-Brown, Joyce; Sharify, Denise Tung; Blake, Bonita; Phillips, Tom; Whitten, Kathleen

    2014-11-06

    Residents of many cities lack affordable, quality housing. Economically disadvantaged neighborhoods often have high rates of poverty and crime, few institutions that enhance the quality of its residents' lives, and unsafe environments for walking and other physical activity. Deteriorating housing contributes to asthma-related illness. We describe the redevelopment of High Point, a West Seattle neighborhood, to improve its built environment, increase neighborhood physical activity, and reduce indoor asthma triggers. High Point is one of Seattle's most demographically diverse neighborhoods. Prior to redevelopment, it had a distressed infrastructure, rising crime rates, and indoor environments that increased asthma-related illness in children and adolescents. High Point residents and partners developed and implemented a comprehensive redevelopment plan to create a sustainable built environment to increase outdoor physical activity and improve indoor environments. We conducted a retrospective analysis of the High Point redevelopment, organized by the different stages of change in the Community Readiness Model. We also examined the multisector partnerships among government and community groups that contributed to the success of the High Point project. Overall quality of life for residents improved as a result of neighborhood redevelopment. Physical activity increased, residents reported fewer days of poor physical or mental health, and social connectedness between neighbors grew. Asthma-friendly homes significantly decreased asthma-related illness among children and adolescents. Providing affordable, quality housing to low-income families improved individual and neighborhood quality of life. Efforts to create social change and improve the health outcomes for entire populations are more effective when multiple organizations work together to improve neighborhood health.

  4. State of the states on brownfields programs for cleanup and reuse of contaminated sites

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    Brownfields consist of land and/or buildings that are abandoned or underutilized where expansion or redevelopment is complicated, in part, because of the threat of known or potential contamination. Federal and state laws governing the treatment of these sites may require remediation (cleanup) of property before redevelopment and can contribute to uncertain liability for property owners or users. Congress, in considering the reauthorization of the Comprehensive Environmental Response, Compensation and Liability Act (CERCLA), commonly known as Superfund, is interested in the issue of brownfields and in their potential return to productive use. As a result, the House Subcommittee on Commerce, Trademore » and Hazardous Materials of the Committee on Commerce requested the Office of Technology Assessment (OTA) to prepare a background paper on issues surrounding cleanup and redevelopment of brownfields.« less

  5. Reversing urban decay: brownfield redevelopment and environmental health.

    PubMed Central

    Greenberg, Michael R

    2003-01-01

    While the United States government concentrates more of its political and financial resources on fighting terrorism, the continuing decay of older cities and industrial suburbs has fallen far down on the national political priority agenda. An exception is the redevelopment of so-called brownfields, which are abandoned, idled, or underutilized factories, railroad yards, bus stations, garages, electricity-generating stations, and other commercial facilities. A modest national government program to identify, clean up, and redevelop brownfields into job fields began during the administration of Bill Clinton and has continued into the George W. Bush administration (Powers et al. 2000; Simons 1998; Van Horn et al. 1999). The political reasons are apparent: Developing brownfields is a politically acceptable method of stimulating private enterprise, local government, and community groups into building new businesses, housing, and community facilities. Also, brownfields projects have a beginning and an end; the national government does not have an indefinite responsibility. In contrast, social assistance programs that grew during the 1960s and proliferated for more than three decades have been politically portrayed by some as give-away programs that build dependency with no ending. Whether this characterization of social programs is morally or empirically justified, the reality is that in today's political environment brownfields redevelopment is a politically acceptable way of helping distressed urban areas. PMID:12573916

  6. Reversing urban decay: brownfield redevelopment and environmental health.

    PubMed

    Greenberg, Michael R

    2003-02-01

    While the United States government concentrates more of its political and financial resources on fighting terrorism, the continuing decay of older cities and industrial suburbs has fallen far down on the national political priority agenda. An exception is the redevelopment of so-called brownfields, which are abandoned, idled, or underutilized factories, railroad yards, bus stations, garages, electricity-generating stations, and other commercial facilities. A modest national government program to identify, clean up, and redevelop brownfields into job fields began during the administration of Bill Clinton and has continued into the George W. Bush administration (Powers et al. 2000; Simons 1998; Van Horn et al. 1999). The political reasons are apparent: Developing brownfields is a politically acceptable method of stimulating private enterprise, local government, and community groups into building new businesses, housing, and community facilities. Also, brownfields projects have a beginning and an end; the national government does not have an indefinite responsibility. In contrast, social assistance programs that grew during the 1960s and proliferated for more than three decades have been politically portrayed by some as give-away programs that build dependency with no ending. Whether this characterization of social programs is morally or empirically justified, the reality is that in today's political environment brownfields redevelopment is a politically acceptable way of helping distressed urban areas.

  7. Dynamics of cross-bridge cycling, ATP hydrolysis, force generation, and deformation in cardiac muscle

    PubMed Central

    Tewari, Shivendra G.; Bugenhagen, Scott M.; Palmer, Bradley M.; Beard, Daniel A.

    2015-01-01

    Despite extensive study over the past six decades the coupling of chemical reaction and mechanical processes in muscle dynamics is not well understood. We lack a theoretical description of how chemical processes (metabolite binding, ATP hydrolysis) influence and are influenced by mechanical processes (deformation and force generation). To address this need, a mathematical model of the muscle cross-bridge (XB) cycle based on Huxley’s sliding filament theory is developed that explicitly accounts for the chemical transformation events and the influence of strain on state transitions. The model is identified based on elastic and viscous moduli data from mouse and rat myocardial strips over a range of perturbation frequencies, and MgATP and inorganic phosphate (Pi) concentrations. Simulations of the identified model reproduce the observed effects of MgATP and MgADP on the rate of force development. Furthermore, simulations reveal that the rate of force re-development measured in slack-restretch experiments is not directly proportional to the rate of XB cycling. For these experiments, the model predicts that the observed increase in the rate of force generation with increased Pi concentration is due to inhibition of cycle turnover by Pi. Finally, the model captures the observed phenomena of force yielding suggesting that it is a result of rapid detachment of stretched attached myosin heads. PMID:25681584

  8. Superfund record of decision amendment (EPA Region 5): H. Brown Company, Inc., Grand Rapids, MI, February 25, 1998

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    This decision document amends the September 29, 1995, Record of Decision (ROD) Amendment for the H. Brown Co., Inc. site, in Walker, Michigan. The major components of the selected remedy include: Consolidating contaminated surface soil and sediment requiring cleanup onto the H. Brown property (2200 Turner Avenue N.W.); Redevelopment of the site, by private parties, with warehousing facilities constructed above the contaminated soil; A cover system comprised of clean fill to develop appropriate grades and elevations, concrete slab foundations, asphalt parking areas, and landscaped areas; Long-term maintenance of the cover system to ensure that the cover will continue to preventmore » direct contact with contaminated soil and minimize infiltration of precipitation; Long-term monitoring of the shallow and intermediate aquifers to monitor the effectiveness of the remedy; Monitoring and/or treatment of landfill gas; Restricting the use of the land and the groundwater; Demolishing on-site buildings to accommodate redevelopment; and Cleanup standards for soil will remain the same as in the 1992 ROD. The purpose of this ROD Amendment is to facilitate the re-development of the H. Brown Co., Inc. Site, and if re-development does not occur or proves to be unsuccessful then the remedy selected in the September 29, 1995 ROD Amendment will be implemented.« less

  9. Early microbial succession in re-developing dental biofilms in periodontal health and disease

    PubMed Central

    TELES, F.R.; TELES, R.P.; UZEL, N.G.; SONG, X.Q.; TORRESYAP, G.; SOCRANSKY, S.S.; HAFFAJEE, A.D.

    2011-01-01

    Objective To determine the order of bacterial species succession in re-developing supra and subgingival biofilms. Methods Supra and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects immediately after professional cleaning. Samples were taken again from 7 teeth in randomly selected quadrants after 1, 2, 4 and 7 days of no oral hygiene and analyzed using checkerboard DNA-DNA hybridization. % DNA probe counts were averaged within subjects at each time point. Ecological succession was determined using a modified moving window analysis. Results Succession in supragingival biofilms from periodontitis and health was similar. At 1 day, Streptococcus mitis and Neisseria mucosa showed increased proportions, followed by Capnocytophaga gingivalis, Eikenella corrodens, Veillonella parvula and Streptococcus oralis at 1–4 days. At 4–7 days, Campylobacter rectus, Campylobacter showae, Prevotella melaninogenica and Prevotella nigrescens became elevated. Subgingival plaque redevelopment was slower and very different from supragingival. Increased proportions were first observed for S. mitis, followed by V. parvula and C. gingivalis and, at 7 days by Capnocytophaga sputigena and P. nigrescens. No significant increase in proportions of periodontal pathogens was observed in any of the clinical groups or locations. Conclusions There is a defined order in bacterial species succession in early supra and subgingival biofilm re-development after professional cleaning. PMID:21895662

  10. Comprehension of handwriting development: Pen-grip kinetics in handwriting tasks and its relation to fine motor skills among school-age children.

    PubMed

    Lin, Yu-Chen; Chao, Yen-Li; Wu, Shyi-Kuen; Lin, Ho-Hsio; Hsu, Chieh-Hsiang; Hsu, Hsiao-Man; Kuo, Li-Chieh

    2017-10-01

    Numerous tools have been developed to evaluate handwriting performances by analysing written products. However, few studies have directly investigated kinetic performances of digits when holding a pen. This study thus attempts to investigate pen-grip kinetics during writing tasks of school-age children and explore the relationship between the kinetic factors and fine motor skills. This study recruited 181 children aged from 5 to 12 years old and investigated the effects of age on handwriting kinetics and the relationship between these and fine motor skills. The forces applied from the digits and pen-tip were measured during writing tasks via a force acquisition pen, and the children's fine motor performances were also evaluated. The results indicate that peak force and average force might not be direct indicators of handwriting performance for normally developing children at this age. Younger children showed larger force variation and lower adjustment frequency during writing, which might indicate they had poorer force control than the older children. Force control when handling a pen is significantly correlated with fine motor performance, especially in relation to the manual dexterity. A novel system is proposed for analysing school-age children's force control while handwriting. We observed the development of force control in relation to pen grip among the children with different ages in this study. The findings suggested that manipulation skill may be crucial when children are establishing their handwriting capabilities. © 2017 Occupational Therapy Australia.

  11. Chemical energetics of force development, force maintenance, and relaxation in mammalian smooth muscle

    PubMed Central

    1980-01-01

    High-energy phosphate utilization (delta approximately P) associated with force development, force maintenance, and relaxation has been determined during single isometric tetani in the rabbit taenia coli. ATP resynthesis from glycolysis and respiration was stopped without deleterious effects on the muscle. At 18 degrees C and a muscle length of 95% l0, the resting rate of energy utilization is 1.8 +/- 0.2 nmol/g . s-1, or 0.85 +/- 0.2 mmol approximately P/mol of total creatine (Ct) . s-1, where Ct = 2.7 mumol/g wet wt. During the initial 25 s of stimulation when force is developed, the average rate of delta approximately P was -8.2 +/- 0.8 mmol/mol Ct . s-1, some four times greater than during the subsequent 35 s of force maintenance, when the rate was -2.0 +/- 0.6 mmol approximately P/mol Ct . s-1. The energy cost of force redevelopment (0 to 95% P0) after a quick release from the peak of a tetanus is very low compared with the initial force development. Therefore, the high rate of energy utilization during force development is not due only to internal work done against the series elasticity nor to any high rate of cross-bridge cycling inherently associated with force development. The high economy of force maintenance compared with other muscle types is undoubtedly due to a slower cross-bridge cycle time. The energy utilization during 45 s of relaxation was not statistically significant, and integral of Pdt/delta approximately P was higher during relaxation than during force maintenance in the stimulated muscle. PMID:6969290

  12. Force feedback effects on single molecule hopping and pulling experiments

    NASA Astrophysics Data System (ADS)

    Rico-Pasto, M.; Pastor, I.; Ritort, F.

    2018-03-01

    Single-molecule experiments with optical tweezers have become an important tool to study the properties and mechanisms of biological systems, such as cells and nucleic acids. In particular, force unzipping experiments have been used to extract the thermodynamics and kinetics of folding and unfolding reactions. In hopping experiments, a molecule executes transitions between the unfolded and folded states at a preset value of the force [constant force mode (CFM) under force feedback] or trap position [passive mode (PM) without feedback] and the force-dependent kinetic rates extracted from the lifetime of each state (CFM) and the rupture force distributions (PM) using the Bell-Evans model. However, hopping experiments in the CFM are known to overestimate molecular distances and folding free energies for fast transitions compared to the response time of the feedback. In contrast, kinetic rate measurements from pulling experiments have been mostly done in the PM while the CFM is seldom implemented in pulling protocols. Here, we carry out hopping and pulling experiments in a short DNA hairpin in the PM and CFM at three different temperatures (6 °C, 25 °C, and 45 °C) exhibiting largely varying kinetic rates. As expected, we find that equilibrium hopping experiments in the CFM and PM perform well at 6 °C (where kinetics are slow), whereas the CFM overestimates molecular parameters at 45 °C (where kinetics are fast). In contrast, nonequilibrium pulling experiments perform well in both modes at all temperatures. This demonstrates that the same kind of feedback algorithm in the CFM leads to more reliable determination of the folding reaction parameters in irreversible pulling experiments.

  13. Force feedback effects on single molecule hopping and pulling experiments.

    PubMed

    Rico-Pasto, M; Pastor, I; Ritort, F

    2018-03-28

    Single-molecule experiments with optical tweezers have become an important tool to study the properties and mechanisms of biological systems, such as cells and nucleic acids. In particular, force unzipping experiments have been used to extract the thermodynamics and kinetics of folding and unfolding reactions. In hopping experiments, a molecule executes transitions between the unfolded and folded states at a preset value of the force [constant force mode (CFM) under force feedback] or trap position [passive mode (PM) without feedback] and the force-dependent kinetic rates extracted from the lifetime of each state (CFM) and the rupture force distributions (PM) using the Bell-Evans model. However, hopping experiments in the CFM are known to overestimate molecular distances and folding free energies for fast transitions compared to the response time of the feedback. In contrast, kinetic rate measurements from pulling experiments have been mostly done in the PM while the CFM is seldom implemented in pulling protocols. Here, we carry out hopping and pulling experiments in a short DNA hairpin in the PM and CFM at three different temperatures (6 °C, 25 °C, and 45 °C) exhibiting largely varying kinetic rates. As expected, we find that equilibrium hopping experiments in the CFM and PM perform well at 6 °C (where kinetics are slow), whereas the CFM overestimates molecular parameters at 45 °C (where kinetics are fast). In contrast, nonequilibrium pulling experiments perform well in both modes at all temperatures. This demonstrates that the same kind of feedback algorithm in the CFM leads to more reliable determination of the folding reaction parameters in irreversible pulling experiments.

  14. Why Did the Storm Ex-Gaston (2010) Fail to Redevelop During the PREDICT Experiment

    DTIC Science & Technology

    2016-07-13

    License. Why did the storm ex-Gaston (2010) fail to redevelop during the PREDICT experiment ? Thomas M. Freismuth1, Blake Rutherford2, Mark A. Boothe1, and...2013), recent case studies in the field in the western North Pacific during the Tropical Cyclone Structure Experiment 2008 (TCS08, Montgomery et al...of ex-Gaston (2010) during the PREDICT experiment is arguably one of the most exten- sively observed non-developing tropical disturbances ever. The 5

  15. Estimation of the energy loss at the blades in rowing: common assumptions revisited.

    PubMed

    Hofmijster, Mathijs; De Koning, Jos; Van Soest, A J

    2010-08-01

    In rowing, power is inevitably lost as kinetic energy is imparted to the water during push-off with the blades. Power loss is estimated from reconstructed blade kinetics and kinematics. Traditionally, it is assumed that the oar is completely rigid and that force acts strictly perpendicular to the blade. The aim of the present study was to evaluate how reconstructed blade kinematics, kinetics, and average power loss are affected by these assumptions. A calibration experiment with instrumented oars and oarlocks was performed to establish relations between measured signals and oar deformation and blade force. Next, an on-water experiment was performed with a single female world-class rower rowing at constant racing pace in an instrumented scull. Blade kinematics, kinetics, and power loss under different assumptions (rigid versus deformable oars; absence or presence of a blade force component parallel to the oar) were reconstructed. Estimated power losses at the blades are 18% higher when parallel blade force is incorporated. Incorporating oar deformation affects reconstructed blade kinematics and instantaneous power loss, but has no effect on estimation of power losses at the blades. Assumptions on oar deformation and blade force direction have implications for the reconstructed blade kinetics and kinematics. Neglecting parallel blade forces leads to a substantial underestimation of power losses at the blades.

  16. The mechanical response of talin

    NASA Astrophysics Data System (ADS)

    Yao, Mingxi; Goult, Benjamin T.; Klapholz, Benjamin; Hu, Xian; Toseland, Christopher P.; Guo, Yingjian; Cong, Peiwen; Sheetz, Michael P.; Yan, Jie

    2016-07-01

    Talin, a force-bearing cytoplasmic adapter essential for integrin-mediated cell adhesion, links the actin cytoskeleton to integrin-based cell-extracellular matrix adhesions at the plasma membrane. Its C-terminal rod domain, which contains 13 helical bundles, plays important roles in mechanosensing during cell adhesion and spreading. However, how the structural stability and transition kinetics of the 13 helical bundles of talin are utilized in the diverse talin-dependent mechanosensing processes remains poorly understood. Here we report the force-dependent unfolding and refolding kinetics of all talin rod domains. Using experimentally determined kinetics parameters, we determined the dynamics of force fluctuation during stretching of talin under physiologically relevant pulling speeds and experimentally measured extension fluctuation trajectories. Our results reveal that force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effective force buffer that sets a physiological force range of only a few pNs in the talin-mediated force transmission pathway.

  17. A software-aided workflow for precinct-scale residential redevelopment

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Glackin, Stephen, E-mail: sglackin@swin.edu.au; Trubka, Roman, E-mail: r.trubka@gmail.com; Dionisio, Maria Rita, E-mail: rita.dionisio@canterbury.ac.nz

    2016-09-15

    Growing urban populations, combined with environmental challenges, have placed significant pressure on urban planning to supply housing while addressing policy issues such as sustainability, affordability, and liveability. The interrelated nature of these issues, combined with the requirement of evidence-based planning, has made decision-making so complex that urban planners need to combine expertise on energy, water, carbon emissions, transport and economic development along with other bodies of knowledge necessary to make well-informed decisions. This paper presents two geospatial software systems that can assist in the mediation of complexity, by allowing users to assess a variety of planning metrics without expert knowledgemore » in those disciplines. Using Envision and Envision Scenario Planner (ESP), both products of the Greening the Greyfields research project funded by the Cooperative Research Centre for Spatial Information (CRCSI) in Australia, we demonstrate a workflow for identifying potential redevelopment precincts and designing and assessing possible redevelopment scenarios to optimise planning outcomes.« less

  18. Impact of Redevelopment Projects on Waste Water Infrastructure

    NASA Astrophysics Data System (ADS)

    Bhave, Prashant; Rahate, Sarvesh

    2018-05-01

    In the last few decades there has been a tremendous increase in urban population globally. Metropolitan cities in India are experiencing rapid change in their population due to migration from rural to urban areas. Due to limited land Mumbai city is experiencing vertical growth in the form of redevelopment projects, signifying a change in population density. Wastewater collection systems greatly contribute to the cost of the overall municipal sewerage system. Present study is an attempt to understand the impact of the redevelopment activities on the wastewater infrastructure. Existing sewerage network of an urban area in Central Mumbai was redesigned and analysed for four different planning scenarios with Bentley's SewerGEM. Results have shown significant change in diameters of the conduits within the sewer network, thus making it inefficient by 13, 19, 31 and 42% with each changing scenario. The results and analysis derived from the study are significant with respect to the urban town planners, developing solutions in alleviating the rising problem of sewer overflows and the economic impact being caused.

  19. Kinetics of molecular transitions with dynamic disorder in single-molecule pulling experiments

    NASA Astrophysics Data System (ADS)

    Zheng, Yue; Li, Ping; Zhao, Nanrong; Hou, Zhonghuai

    2013-05-01

    Macromolecular transitions are subject to large fluctuations of rate constant, termed as dynamic disorder. The individual or intrinsic transition rates and activation free energies can be extracted from single-molecule pulling experiments. Here we present a theoretical framework based on a generalized Langevin equation with fractional Gaussian noise and power-law memory kernel to study the kinetics of macromolecular transitions to address the effects of dynamic disorder on barrier-crossing kinetics under external pulling force. By using the Kramers' rate theory, we have calculated the fluctuating rate constant of molecular transition, as well as the experimentally accessible quantities such as the force-dependent mean lifetime, the rupture force distribution, and the speed-dependent mean rupture force. Particular attention is paid to the discrepancies between the kinetics with and without dynamic disorder. We demonstrate that these discrepancies show strong and nontrivial dependence on the external force or the pulling speed, as well as the barrier height of the potential of mean force. Our results suggest that dynamic disorder is an important factor that should be taken into account properly in accurate interpretations of single-molecule pulling experiments.

  20. Probing static disorder in Arrhenius kinetics by single-molecule force spectroscopy.

    PubMed

    Kuo, Tzu-Ling; Garcia-Manyes, Sergi; Li, Jingyuan; Barel, Itay; Lu, Hui; Berne, Bruce J; Urbakh, Michael; Klafter, Joseph; Fernández, Julio M

    2010-06-22

    The widely used Arrhenius equation describes the kinetics of simple two-state reactions, with the implicit assumption of a single transition state with a well-defined activation energy barrier DeltaE, as the rate-limiting step. However, it has become increasingly clear that the saddle point of the free-energy surface in most reactions is populated by ensembles of conformations, leading to nonexponential kinetics. Here we present a theory that generalizes the Arrhenius equation to include static disorder of conformational degrees of freedom as a function of an external perturbation to fully account for a diverse set of transition states. The effect of a perturbation on static disorder is best examined at the single-molecule level. Here we use force-clamp spectroscopy to study the nonexponential kinetics of single ubiquitin proteins unfolding under force. We find that the measured variance in DeltaE shows both force-dependent and independent components, where the force-dependent component scales with F(2), in excellent agreement with our theory. Our study illustrates a novel adaptation of the classical Arrhenius equation that accounts for the microscopic origins of nonexponential kinetics, which are essential in understanding the rapidly growing body of single-molecule data.

  1. Re-development of mental health first aid guidelines for suicidal ideation and behaviour: a Delphi study.

    PubMed

    Ross, Anna M; Kelly, Claire M; Jorm, Anthony F

    2014-09-13

    Suicide continues to be a leading cause of death globally. Friends and family are considered best positioned to provide initial assistance if someone is suicidal. Expert consensus guidelines on how to do this were published in 2008. Re-developing these guidelines is necessary to ensure they contain the most current recommended helping actions and remain consistent with the suicide prevention literature. The Delphi consensus method was used to determine the importance of including helping statements in the guidelines. These statements describe helping actions a member of the public can take, and information they should have, to help someone who is experiencing suicidal thoughts. Systematic searches of the available suicide prevention literature were carried out to find helping statements. Two expert panels, comprising 41 suicide prevention professionals and 35 consumer advocates respectively, rated each statement. Statements were accepted for inclusion in the guidelines if they were endorsed by at least 80% of each panel. Out of 436 statements, 164 were endorsed as appropriate helping actions in providing assistance to someone experiencing suicidal thoughts or engaging in suicidal behaviour. These statements were used to form the re-developed guidelines. The re-development of the guidelines has resulted in more comprehensive guidance than the earlier version, with the endorsement of 164 helping actions, compared to 30 previously. These guidelines will form the basis of a suicide prevention course aimed at educating members of the public on providing first aid to someone who is experiencing suicidal thoughts.

  2. Thermodynamically Feasible Kinetic Models of Reaction Networks

    PubMed Central

    Ederer, Michael; Gilles, Ernst Dieter

    2007-01-01

    The dynamics of biological reaction networks are strongly constrained by thermodynamics. An holistic understanding of their behavior and regulation requires mathematical models that observe these constraints. However, kinetic models may easily violate the constraints imposed by the principle of detailed balance, if no special care is taken. Detailed balance demands that in thermodynamic equilibrium all fluxes vanish. We introduce a thermodynamic-kinetic modeling (TKM) formalism that adapts the concepts of potentials and forces from irreversible thermodynamics to kinetic modeling. In the proposed formalism, the thermokinetic potential of a compound is proportional to its concentration. The proportionality factor is a compound-specific parameter called capacity. The thermokinetic force of a reaction is a function of the potentials. Every reaction has a resistance that is the ratio of thermokinetic force and reaction rate. For mass-action type kinetics, the resistances are constant. Since it relies on the thermodynamic concept of potentials and forces, the TKM formalism structurally observes detailed balance for all values of capacities and resistances. Thus, it provides an easy way to formulate physically feasible, kinetic models of biological reaction networks. The TKM formalism is useful for modeling large biological networks that are subject to many detailed balance relations. PMID:17208985

  3. Repair, Evaluation, Maintenance, and Rehabilitation Research Program: Redevelopment of Relief Wells, Upper Wood River Drainage and Levee District, Madison County, Illinois

    DTIC Science & Technology

    1993-11-01

    indicate that the wel rnproved to nearly their original effec- tiveness as a result of the prolongf., eriod of flow during the flood. Evalua- tion of... flow data from the Flood of. )73 indicated restored yields in some of the test wells of slightly higher than 100 percent of the original values. Tests...redevelopment and flushing of bacterial residue occurred as a result of the high flows . The same studies indicated that the test wells of the Upper Wood

  4. Writing Forces Associated With Four Pencil Grasp Patterns in Grade 4 Children

    PubMed Central

    Schwellnus, Heidi; Carnahan, Heather; Kushki, Azadeh; Polatajko, Helene; Missiuna, Cheryl

    2013-01-01

    OBJECTIVE. We investigated differences in handwriting kinetics, speed, and legibility among four pencil grasps after a 10-min copy task. METHOD. Seventy-four Grade 4 students completed a handwriting assessment before and after a copy task. Grip and axial forces were measured with an instrumented stylus and force-sensitive tablet. We used multiple linear regression to analyze the relationship between grasp pattern and grip and axial forces. RESULTS. We found no kinetic differences among grasps, whether considered individually or grouped by the number of fingers on the barrel. However, when grasps were grouped according to the thumb position, the adducted grasps exhibited higher mean grip and axial forces. CONCLUSION. Grip forces were generally similar across the different grasps. Kinetic differences resulting from thumb position seemed to have no bearing on speed and legibility. Interventions for handwriting difficulties should focus more on speed and letter formation than on grasp pattern. PMID:23433277

  5. Soil Contamination as a Legacy of the U.S. Auto Industry, Southwest Detroit, Michigan USA

    NASA Astrophysics Data System (ADS)

    Murray, K.; Peterman, C.

    2012-04-01

    The Delray community of southwest Detroit is one of the most depressed areas in southeast Michigan. Historically, Delray was a working class, racially diverse community that depended heavily on industrial jobs provided by nearby factories. However, decades of industrial waste discharges have left Delray with extensive air and soil pollution. Although high unemployment and poverty are major challenges confronting residents in Delray today, the threat to public health from Pb, Hg, As and Cr [VI] in the soil may become an even bigger issue and a significant source of concern. Newspaper headlines cite crime, substance abuse, high school and labor force dropout, as being prevalent in Delray, but recent research suggest that soil contamination, which has resulted in elevated blood Pb levels may be an underlying factor. Recent interest in this area as a potential site for a new bridge to Canada, has offered new hope to the residents by potentially opening the door for redevelopment. The initial step in this process is an environmental assessment of the Delray community. This investigation is being conducted by the University of Michigan-Dearborn in consortium with local community groups and the Detroit Public School. Although preliminary, an analysis of soil samples from over 400 residences has indicated that significant levels of As, and Pb are present in the upper 0.1 meter of soil throughout the Delray area. The high levels of metals present in the soil suggest that further investigation and possible remedial action will be necessary prior to redevelopment.

  6. Ground reaction forces and knee kinetics during single and repeated badminton lunges.

    PubMed

    Lam, Wing Kai; Ding, Rui; Qu, Yi

    2017-03-01

    Repeated movement (RM) lunge that frequently executed in badminton might be used for footwear evaluation. This study examined the influence of single movement (SM) and RM lunges on the ground reaction forces (GRFs) and knee kinetics during the braking phase of a badminton lunge step. Thirteen male university badminton players performed left-forward lunges in both SM and RM sessions. Force platform and motion capturing system were used to measure GRFs and knee kinetics variables. Paired t-test was performed to determine any significant differences between SM and RM lunges regarding mean and coefficient of variation (CV) in each variable. The kinetics results indicated that compared to SM lunges, the RM lunges had shorter contact time and generated smaller maximum loading rate of impact force, peak knee anterior-posterior force, and peak knee sagittal moment but generated larger peak horizontal resultant forces (Ps < 0.05). Additionally, the RM lunges had lower CV for peak knee medial-lateral and vertical forces (Ps < 0.05). These results suggested that the RM testing protocols had a distinct loading response and adaptation pattern during lunge and that the RM protocol showed higher within-trial reliability, which may be beneficial for the knee joint loading evaluation under different interventions.

  7. Re-development of mental health first aid guidelines for supporting Aboriginal and Torres Strait Islanders who are engaging in non-suicidal self-injury.

    PubMed

    Armstrong, Gregory; Ironfield, Natalie; Kelly, Claire M; Dart, Katrina; Arabena, Kerry; Bond, Kathy; Jorm, Anthony F

    2017-08-22

    Non-suicidal self-injury (NSSI) disproportionally affects Indigenous Australians. Friends, family and frontline workers (for example, teachers, youth workers) are often best positioned to provide initial assistance if someone is engaging in NSSI. Culturally appropriate expert consensus guidelines on how to provide mental health first aid to Australian Aboriginal and Torres Strait Islanders who are engaging in NSSI were developed in 2009. This study describes the re-development of these guidelines to ensure they contain the most current recommended helping actions. The Delphi consensus method was used to elicit consensus on potential helping statements to be included in the guidelines. These statements describe helping actions that Indigenous community members and non-Indigenous frontline workers can take, and information they should have, to help someone who is engaging in NSSI. The statements were sourced from systematic searches of peer-reviewed literature, grey literature, books, websites and online materials, and existing NSSI courses. A panel was formed, comprising 26 Aboriginal and Torres Strait Islanders with expertise in NSSI. The panellists were presented with the helping statements via online questionnaires and were encouraged to suggest re-wording of statements and any additional helping statements that were not included in the original questionnaire. Statements were only accepted for inclusion in the guidelines if they were endorsed by ≥90% of panellists as essential or important. From a total of 185 statements shown to the expert panel, 115 were endorsed as helping statements to be included in the re-developed guidelines. A panel of Aboriginal and Torres Strait Islander people with expertise in NSSI were able to reach consensus on appropriate strategies for providing mental health first aid to an Aboriginal and Torres Strait Islander engaging in NSSI. The re-development of the guidelines has resulted in more comprehensive guidance than the earlier version. The re-developed guidelines will form the basis of an Aboriginal mental health first aid short course on NSSI for Indigenous community members and non-Indigenous frontline workers that will be evaluated in an upcoming trial.

  8. Explosive sport training and torque kinetics in children.

    PubMed

    Dotan, Raffy; Mitchell, Cameron J; Cohen, Rotem; Gabriel, David; Klentrou, Panagiota; Falk, Bareket

    2013-07-01

    A high rate of force development (RFD) is often more important than maximal force in daily and sports activities. In children, resistance training has been shown to increase maximal force. It is unclear whether, or to what extent, can children improve RFD and force kinetics. For this study, we compared strength and force kinetics of boy gymnasts with those of untrained boys and untrained men. Eight boy gymnasts (age, 9.5 ± 1.2 y), 20 untrained boys (age, 10.1 ± 1.3 y), and 20 untrained men (age, 22.9 ± 4.4 y) performed maximal, explosive, isometric elbow flexions (EF) and knee flexions (KF). Peak torque (maximal voluntary contraction (MVC)), elapsed times to 10%-100% MVC, peak rate of torque development (RTDpk), and other kinetics parameters were determined. When gymnasts were compared with untrained boys, size-normalized EF MVC was 11%-20% higher, RTDpk was 32% higher, and times to 30% and 80% MVC were 16% and 55% shorter, respectively (p < 0.05). No corresponding differences were observed in KF. Furthermore, although the normalized EF MVC was 28% lower in gymnasts than in men (p < 0.001), their torque kinetics parameters were similar. These findings highlight the specificity of gymnastics training, which markedly elevated the torque kinetics of young, prepubertal boys to adult levels, but only moderately affected peak torque. It is suggested that neurologic adaptations, such as enhanced firing and activation rates or increased type II motor-unit recruitment, as well as changes in musculotendinous stiffness, could explain these findings.

  9. Design and implementation of an air monitoring program in support of a brownfields redevelopment program

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Maisel, B.E.; Hunt, G.T.; Devaney, R.J. Jr.

    EPA`s Brownfields Economic Redevelopment Initiative has sparked renewal of industrial and commercial parcels otherwise idled or under-utilized because of real or perceived environmental contamination. In certain cases, restoring such parcels to productive economic use requires a redevelopment effort protective of human health and welfare through minimizing offsite migration of environmental contaminants during cleanup, demolition and remediation activities. To support these objectives, an air monitoring program is often required as an integral element of a comprehensive brownfields redevelopment effort. This paper presents a strategic framework for design and execution of an ambient air monitoring program in support of a brownfields remediationmore » effort ongoing in Lawrence, MA. Based on site characterization, the program included sample collection and laboratory analysis of ambient air samples for polychlorinated biphenyls (PCBs), polychlorinated dibenzodioxins and polychlorinated dibenzofurans (PCDDs/PCDFs), total suspended particulate (TSP), inhalable particulate (PM10), and lead. The program included four monitoring phases, identified as background, wintertime, demolition/remediation and post-demolition. Air sampling occurred over a 16 month period during 1996--97, during which time nine sampling locations were utilized to produce approximately 1,500 ambient air samples. Following strict data review and validation procedures, ambient air data interpretation focused on the following: evaluation of upwind/downwind sample pairs, comparison of ambient levels to existing regulatory standards, relation of ambient levels to data reported in the open literature, and, determination of normal seasonal variations in existing background burden, comparison of ambient levels measured during site activity to background levels.« less

  10. Early microbial succession in redeveloping dental biofilms in periodontal health and disease.

    PubMed

    Teles, F R; Teles, R P; Uzel, N G; Song, X Q; Torresyap, G; Socransky, S S; Haffajee, A D

    2012-02-01

    The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subjects or if there are differences in bacterial recolonization between supragingival and subgingival biofilms in periodontal health and disease. Supragingival and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects immediately after professional cleaning. Samples were taken again from seven teeth in randomly selected quadrants after 1, 2, 4 and 7 d of no oral hygiene and analyzed using checkerboard DNA-DNA hybridization. The percentage of DNA probe counts were averaged within subjects at each time-point. Ecological succession was determined using a modified moving-window analysis. Succession in supragingival biofilms from subjects with periodontitis and from healthy individuals was similar. At 1 d, Streptococcus mitis and Neisseria mucosa showed increased proportions, followed by Capnocytophaga gingivalis, Eikenella corrodens, Veillonella parvula and Streptococcus oralis at 1-4 d. At 4-7 d, Campylobacter rectus, Campylobacter showae, Prevotella melaninogenica and Prevotella nigrescens became elevated. Subgingival plaque redevelopment was slower and very different from supragingival plaque redevelopment. Increased proportions were first observed for S. mitis, followed by V. parvula and C. gingivalis and, at 7 d, by Capnocytophaga sputigena and P. nigrescens. No significant increase in the proportions of periodontal pathogens was observed in any of the clinical groups or locations. There is a defined order in bacterial species succession in early supragingival and subgingival biofilm redevelopment after professional cleaning. © 2011 John Wiley & Sons A/S.

  11. Kinetic-energy absorber employs frictional force between mating cylinders

    NASA Technical Reports Server (NTRS)

    Conrad, E. W.

    1964-01-01

    A kinetic energy absorbing device uses a series of coaxial, mating cylindrical surfaces. These surfaces have high frictional resistance to relative motion when axial impact forces are applied. The device is designed for safe deceleration of vehicles impacting on landing surfaces.

  12. Partially ionized gas flow and heat transfer in the separation, reattachment, and redevelopment regions downstream of an abrupt circular channel expansion.

    NASA Technical Reports Server (NTRS)

    Back, L. H.; Massier, P. F.; Roschke, E. J.

    1972-01-01

    Heat transfer and pressure measurements obtained in the separation, reattachment, and redevelopment regions along a tube and nozzle located downstream of an abrupt channel expansion are presented for a very high enthalpy flow of argon. The ionization energy fraction extended up to 0.6 at the tube inlet just downstream of the arc heater. Reattachment resulted from the growth of an instability in the vortex sheet-like shear layer between the central jet that discharged into the tube and the reverse flow along the wall at the lower Reynolds numbers, as indicated by water flow visualization studies which were found to dynamically model the high-temperature gas flow. A reasonably good prediction of the heat transfer in the reattachment region where the highest heat transfer occurred and in the redevelopment region downstream can be made by using existing laminar boundary layer theory for a partially ionized gas. In the experiments as much as 90 per cent of the inlet energy was lost by heat transfer to the tube and the nozzle wall.

  13. The Chicago Center for Green Technology: life-cycle assessment of a brownfield redevelopment project

    NASA Astrophysics Data System (ADS)

    Brecheisen, Thomas; Theis, Thomas

    2013-03-01

    The sustainable development of brownfields reflects a fundamental, yet logical, shift in thinking and policymaking regarding pollution prevention. Life-cycle assessment (LCA) is a tool that can be used to assist in determining the conformity of brownfield development projects to the sustainability paradigm. LCA was applied to the process of a real brownfield redevelopment project, now known as the Chicago Center for Green Technology, to determine the cumulative energy required to complete the following redevelopment stages: (1) brownfield assessment and remediation, (2) building rehabilitation and site development and (3) ten years of operation. The results of the LCA have shown that operational energy is the dominant life-cycle stage after ten years of operation. The preservation and rehabilitation of the existing building, the installation of renewable energy systems (geothermal and photovoltaic) on-site and the use of more sustainable building products resulted in 72 terajoules (TJ) of avoided energy impacts, which would provide 14 years of operational energy for the site. Methodological note: data for this life-cycle assessment were obtained from project reports, construction blueprints and utility bills.

  14. Why did the storm ex-Gaston (2010) fail to redevelop during the PREDICT experiment?

    NASA Astrophysics Data System (ADS)

    Freismuth, Thomas M.; Rutherford, Blake; Boothe, Mark A.; Montgomery, Michael T.

    2016-07-01

    An analysis is presented of the failed re-development of ex-Gaston during the 2010 PREDICT field campaign based on the European Centre for Medium Range Weather Forecast (ECMWF) analyses. We analyze the dynamics and kinematics of ex-Gaston to investigate the role of dry, environmental air in the failed redevelopment. The flow topology defined by the calculation of particle trajectories shows that ex-Gaston's pouch was vulnerable to dry, environmental air on all days of observations. As early as 12:00 UTC 2 September 2010, a dry layer at and above 600 hPa results in a decrease in the vertical mass flux and vertical relative vorticity. These findings support the hypothesis that entrained, dry air near 600 hPa thwarted convective updraughts and vertical mass flux, which in turn led to a reduction in vorticity and a compromised pouch at these middle levels. A compromised pouch allows further intrusion of dry air and quenching of subsequent convection, therefore hindering vorticity amplification through vortex tube stretching. This study supports recent work investigating the role of dry air in moist convection during tropical cyclogenesis.

  15. Reviving old antibiotics.

    PubMed

    Theuretzbacher, Ursula; Van Bambeke, Françoise; Cantón, Rafael; Giske, Christian G; Mouton, Johan W; Nation, Roger L; Paul, Mical; Turnidge, John D; Kahlmeter, Gunnar

    2015-08-01

    In the face of increasing antimicrobial resistance and the paucity of new antimicrobial agents it has become clear that new antimicrobial strategies are urgently needed. One of these is to revisit old antibiotics to ensure that they are used correctly and to their full potential, as well as to determine whether one or several of them can help alleviate the pressure on more recent agents. Strategies are urgently needed to 're-develop' these drugs using modern standards, integrating new knowledge into regulatory frameworks and communicating the knowledge from the research bench to the bedside. Without a systematic approach to re-developing these old drugs and rigorously testing them according to today's standards, there is a significant risk of doing harm to patients and further increasing multidrug resistance. This paper describes factors to be considered and outlines steps and actions needed to re-develop old antibiotics so that they can be used effectively for the treatment of infections. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  16. Derby district redevelopment in Colorado: case study on the health impact assessment process.

    PubMed

    Maclennan, Carol F; Ghosh, Tista S; Juliusson, Lara; Vogt, Richard L; Boehmer, Tegan K

    2012-01-01

    Health Impact Assessment (HIA) is a tool that is increasingly utilized in the U.S. to shape policies that may impact the public's health. Domestic examples of HIAs and the process by which they were conducted, however, are rarely documented in the peer-reviewed literature. Through an existing relationship with the planning department in Commerce City, Colorado, Tri-County Health Department (TCHD) was able to identify a proposed redevelopment plan as a candidate for an HIA. The HIA focused on potential effects of the proposed redevelopment of Commerce City's historic Derby District on residents' physical activity and nutrition-related behaviors. This article describes the HIA process used by TCHD. Several sources of data were used, including participatory community input on walkability and safety, local health behavior data, and maps of health-influencing environmental characteristics. Using a variety of information sources including community input and local health behavior data can be useful in conducting HIAs and impacting policies. Local health departments should consider cultivating ongoing collaborative partnerships with municipal planning departments and community groups to conduct HIAs and to implement recommendations.

  17. Upper limb joint kinetic analysis during tennis serve: Assessment of competitive level on efficiency and injury risks.

    PubMed

    Martin, C; Bideau, B; Ropars, M; Delamarche, P; Kulpa, R

    2014-08-01

    The aim of this work was to compare the joint kinetics and stroke production efficiency for the shoulder, elbow, and wrist during the serve between professionals and advanced tennis players and to discuss their potential relationship with given overuse injuries. Eleven professional and seven advanced tennis players were studied with an optoelectronic motion analysis system while performing serves. Normalized peak kinetic values of the shoulder, elbow, and wrist joints were calculated using inverse dynamics. To measure serve efficiency, all normalized peak kinetic values were divided by ball velocity. t-tests were used to determine significant differences between the resultant joint kinetics and efficiency values in both groups (advanced vs professional). Shoulder inferior force, shoulder anterior force, shoulder horizontal abduction torque, and elbow medial force were significantly higher in advanced players. Professional players were more efficient than advanced players, as they maximize ball velocity with lower joint kinetics. Since advanced players are subjected to higher joint kinetics, the results suggest that they appeared more susceptible to high risk of shoulder and elbow injuries than professionals, especially during the cocking and deceleration phases of the serve. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  18. The project management office: transforming healthcare in the context of a hospital redevelopment project.

    PubMed

    Richer, Marie-Claire; Marchionni, Caroline; Lavoie-Tremblay, Melanie; Aubry, Monique

    2013-01-01

    It has been shown that classifying projects into a typology allows improved allocation of resources and promotes project success. However, a typology of healthcare projects has yet to be developed. The projects encountered by the Transition Support Office at the McGill University Health Centre in Montreal, Quebec, where a major redevelopment project is under way, were classified into a typology unique to the healthcare context. Examples of the 3 project types, Process, People, and Practice, are provided to clarify the specific support strategies and context-adapted interventions that were instrumental to their success.

  19. Writing forces associated with four pencil grasp patterns in grade 4 children.

    PubMed

    Schwellnus, Heidi; Carnahan, Heather; Kushki, Azadeh; Polatajko, Helene; Missiuna, Cheryl; Chau, Tom

    2013-01-01

    OBJECTIVE. We investigated differences in handwriting kinetics, speed, and legibility among four pencil grasps after a 10-min copy task. METHOD. Seventy-four Grade 4 students completed a handwriting assessment before and after a copy task. Grip and axial forces were measured with an instrumented stylus and force-sensitive tablet. We used multiple linear regression to analyze the relationship between grasp pattern and grip and axial forces. RESULTS. We found no kinetic differences among grasps, whether considered individually or grouped by the number of fingers on the barrel. However, when grasps were grouped according to the thumb position, the adducted grasps exhibited higher mean grip and axial forces. CONCLUSION. Grip forces were generally similar across the different grasps. Kinetic differences resulting from thumb position seemed to have no bearing on speed and legibility. Interventions for handwriting difficulties should focus more on speed and letter formation than on grasp pattern. Copyright © 2013 by the American Occupational Therapy Association, Inc.

  20. Dynamically adjustable foot-ground contact model to estimate ground reaction force during walking and running.

    PubMed

    Jung, Yihwan; Jung, Moonki; Ryu, Jiseon; Yoon, Sukhoon; Park, Sang-Kyoon; Koo, Seungbum

    2016-03-01

    Human dynamic models have been used to estimate joint kinetics during various activities. Kinetics estimation is in demand in sports and clinical applications where data on external forces, such as the ground reaction force (GRF), are not available. The purpose of this study was to estimate the GRF during gait by utilizing distance- and velocity-dependent force models between the foot and ground in an inverse-dynamics-based optimization. Ten males were tested as they walked at four different speeds on a force plate-embedded treadmill system. The full-GRF model whose foot-ground reaction elements were dynamically adjusted according to vertical displacement and anterior-posterior speed between the foot and ground was implemented in a full-body skeletal model. The model estimated the vertical and shear forces of the GRF from body kinematics. The shear-GRF model with dynamically adjustable shear reaction elements according to the input vertical force was also implemented in the foot of a full-body skeletal model. Shear forces of the GRF were estimated from body kinematics, vertical GRF, and center of pressure. The estimated full GRF had the lowest root mean square (RMS) errors at the slow walking speed (1.0m/s) with 4.2, 1.3, and 5.7% BW for anterior-posterior, medial-lateral, and vertical forces, respectively. The estimated shear forces were not significantly different between the full-GRF and shear-GRF models, but the RMS errors of the estimated knee joint kinetics were significantly lower for the shear-GRF model. Providing COP and vertical GRF with sensors, such as an insole-type pressure mat, can help estimate shear forces of the GRF and increase accuracy for estimation of joint kinetics. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Spin force and torque in non-relativistic Dirac oscillator on a sphere

    NASA Astrophysics Data System (ADS)

    Shikakhwa, M. S.

    2018-03-01

    The spin force operator on a non-relativistic Dirac oscillator (in the non-relativistic limit the Dirac oscillator is a spin one-half 3D harmonic oscillator with strong spin-orbit interaction) is derived using the Heisenberg equations of motion and is seen to be formally similar to the force by the electromagnetic field on a moving charged particle. When confined to a sphere of radius R, it is shown that the Hamiltonian of this non-relativistic oscillator can be expressed as a mere kinetic energy operator with an anomalous part. As a result, the power by the spin force and torque operators in this case are seen to vanish. The spin force operator on the sphere is calculated explicitly and its torque is shown to be equal to the rate of change of the kinetic orbital angular momentum operator, again with an anomalous part. This, along with the conservation of the total angular momentum, suggests that the spin force exerts a spin-dependent torque on the kinetic orbital angular momentum operator in order to conserve total angular momentum. The presence of an anomalous spin part in the kinetic orbital angular momentum operator gives rise to an oscillatory behavior similar to the Zitterbewegung. It is suggested that the underlying physics that gives rise to the spin force and the Zitterbewegung is one and the same in NRDO and in systems that manifest spin Hall effect.

  2. The effect of ankle bracing on knee kinetics and kinematics during volleyball-specific tasks.

    PubMed

    West, T; Ng, L; Campbell, A

    2014-12-01

    The purpose of this study was to examine the effects of ankle bracing on knee kinetics and kinematics during volleyball tasks. Fifteen healthy, elite, female volleyball players performed a series of straight-line and lateral volleyball tasks with no brace and when wearing an ankle brace. A 14-camera Vicon motion analysis system and AMTI force plate were used to capture the kinetic and kinematic data. Knee range of motion, peak knee anterior-posterior and medial-lateral shear forces, and peak ground reaction forces that occurred between initial contact with the force plate and toe off were compared using paired sample t-tests between the braced and non-braced conditions (P < 0.05). The results revealed no significant effect of bracing on knee kinematics or ground reaction forces during any task or on knee kinetics during the straight-line movement volleyball tasks. However, ankle bracing was demonstrated to reduce knee lateral shear forces during all of the lateral movement volleyball tasks. Wearing the Active Ankle T2 brace will not impact knee joint range of motion and may in fact reduce shear loading to the knee joint in volleyball players. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  3. Active mechanics in living oocytes reveal molecular-scale force kinetics

    NASA Astrophysics Data System (ADS)

    Ahmed, Wylie; Fodor, Etienne; Almonacid, Maria; Bussonnier, Matthias; Verlhac, Marie-Helene; Gov, Nir; Visco, Paolo; van Wijland, Frederic; Betz, Timo

    Unlike traditional materials, living cells actively generate forces at the molecular scale that change their structure and mechanical properties. This nonequilibrium activity is essential for cellular function, and drives processes such as cell division. Single molecule studies have uncovered the detailed force kinetics of isolated motor proteins in-vitro, however their behavior in-vivo has been elusive due to the complex environment inside the cell. Here, we quantify active forces and intracellular mechanics in living oocytes using in-vivo optical trapping and laser interferometry of endogenous vesicles. We integrate an experimental and theoretical framework to connect mesoscopic measurements of nonequilibrium properties to the underlying molecular- scale force kinetics. Our results show that force generation by myosin-V drives the cytoplasmic-skeleton out-of-equilibrium (at frequencies below 300 Hz) and actively softens the environment. In vivo myosin-V activity generates a force of F ~ 0 . 4 pN, with a power-stroke of length Δx ~ 20 nm and duration τ ~ 300 μs, that drives vesicle motion at vv ~ 320 nm/s. This framework is widely applicable to characterize living cells and other soft active materials.

  4. Child–adult differences in the kinetics of torque development

    PubMed Central

    DOTAN, RAFFY; MITCHELL, CAMERON; COHEN, ROTEM; GABRIEL, DAVID; KLENTROU, PANAGIOTA; FALK, BAREKET

    2013-01-01

    Children have lower size-normalised maximal voluntary force, speed, and power than adults. It has been hypothesised that these and other age-related performance differences are due to lesser type-II motor-unit utilisation in children. This should be manifested as slower force kinetics in explosive muscle contractions. The purpose of this study was to investigate the nature of child–adult force-kinetics differences and whether the latter could support that hypothesis. Untrained boys (n = 20) and men (n = 20) (10.1 ± 1.3 and 22.9 ± 4.4 years, respectively), performed maximal, explosive, isometric elbow flexions and knee extensions on a Biodex dynamometer. Peak torque (MVC), times to 10–100% MVC, and other kinetics parameters were determined. The boys’ body-mass-normalised knee extension MVC, peak rate of torque development, and %MVC at 100 ms were 26, 17 and 23% lower compared with the men and their times to 30% and 80% MVC were 24 and 48% longer, respectively. Elbow flexion kinetics showed similar or greater differences. The findings illuminate boys’ inherent disadvantage in tasks requiring speed or explosive force. It is demonstrated that the extent of the boys–men kinetics disparity cannot be explained by muscle-composition and/or musculo-tendinous-stiffness differences. We suggest therefore that the findings indirectly support children’s lower utilisation of type-II motor units. PMID:23320937

  5. Modeling and Management of Increased Urban Stormwater Runoff Using InfoSWMM Sustain in the Berkeley Neighborhood of Denver, Colorado

    NASA Astrophysics Data System (ADS)

    Panos, C.; Hogue, T. S.; McCray, J. E.

    2016-12-01

    Few urban studies have evaluated the hydrologic impacts of redevelopment - for example, a rapid conversion from single to multi-family homes - known as infill, or re-urbanization. Redevelopment provides unique stormwater challenges as private property owners in many cities are not mandated to undertake stormwater retrofits leading to an overall increase in stormwater quantity and decrease in quality. This research utilizes a version of the EPA's Storm Water Management Model (SWMM), InfoSWMM Sustain, to model and analyze the impacts of impervious cover change due to redevelopment on stormwater quantity and quality in Denver, Colorado, with a focus on the Berkeley Neighborhood, where the percent imperviousness is expected to increase significantly from a current value of 53% by 2025. We utilize flow data from multiple pressure transducers installed directly within the storm sewer network as well as water quality data from storm and low flow sampling to initially calibrate InfoSWMM Sustain using September 2015 through September 2016 storm data. Model scenarios include current land cover conditions as well as future imperviousness predictions from redevelopment. The Urban Drainage and Flood Control District's Colorado Urban Hydrograph Procedure (CUHP) model is also implemented and used for calibration and comparison to the InfoSWMM stormwater model. Model simulations predicting an average annual stormwater runoff for the basin will be used to inform stormwater capture for the Berkeley Neighborhood on the downstream Willis Case Golf Course, where treatment trains are being designed to provide irrigation water (a 250 ac-ft per year demand) and improved water quality for discharge to the nearby receiving waters of Clear Creek. Ultimately, study results will better inform regional stormwater capture requirements when transitioning from single to multi-family units by providing a quantitative basis for treatment and regulation priorities.

  6. Developing lay health worker policy in South Africa: a qualitative study

    PubMed Central

    2012-01-01

    Background Over the past half decade South Africa has been developing, implementing and redeveloping its Lay Health Worker (LHW) policies. Research during this period has highlighted challenges with LHW programme implementation. These challenges have included an increased burden of care for female LHWs. The aim of this study was to explore contemporary LHW policy development processes and the extent to which issues of gender are taken up within this process. Methods The study adopted a qualitative approach to exploring policy development from the perspective of policy actors. Eleven policy actors (policy makers and policy commentators) were interviewed individually. Data from the interviews were analysed thematically. Results Considerations of LHW working conditions drove policy redevelopment. From the interviews it seems that gender as an issue never reached the policy making agenda. Although there was strong recognition that the working conditions of LHWs needed to be improved, poor working conditions were not necessarily seen as a gender concern. Our data suggests that in the process of defining the problem which the redeveloped policy had to address, gender was not included. There was no group or body who brought the issue of gender to the attention of policy developers. As such the issue of gender never entered the policy debates. These debates focused on whether it was appropriate to have LHWs, what LHW programme model should be adopted and whether or not LHWs should be incorporated into the formal health system. Conclusion LHW policy redevelopment focused on resolving issues of LHW working conditions through an active process involving many actors and strong debates. Within this process the issue of gender had no champion and never reached the LHW policy agenda. Future research may consider how to incorporate the voices of ordinary women into the policy making process. PMID:22410185

  7. Kinetic factors of vertical jumping for heading a ball in flexible flatfooted amateur soccer players with and without insole adoption.

    PubMed

    Arastoo, Ali Asghar; Aghdam, Esmaeil Moharrami; Habibi, Abdoul Hamid; Zahednejad, Shahla

    2014-06-01

    According to literature, little is known regarding the effects of orthotic management of flatfoot on kinetics of vertical jump. To compare the kinetic and temporal events of two-legged vertical jumping take-off from a force plate for heading a ball in normal and flexible flatfoot subjects with and without insole. A functional based interventional controlled study. Random sampling method was employed to draw a control group of 15 normal foot subjects to a group of 15 flatfoot subjects. A force platform was used to record kinetics of two-legged vertical jump shots. Results indicate that insole did not lead to a significant effect on kinetics regarding anterior-posterior and mediolateral directions (p > 0.05). Results of kinetics related to vertical direction for maximum force due to take-off and stance duration revealed significant differences between the normal and flexible flatfoot subjects without insole (p < 0.05) and no significant differences between the normal foot and flexible flatfoot subjects with insole adoption (p > 0.05). These results suggest that the use of an insole in the flexible flatfoot subjects led to improved stance time and decrease of magnitude of kinetics regarding vertical direction at take-off as the main feature of two-legged vertical jumping function. Adoption of the insole improved the design of the shoe-foot interface support for the flexible flatfoot athletes, enabling them to develop more effective take-off kinetics for vertical jumping in terms of ground reaction force and stance duration similar to that of normal foot subjects without insole. © The International Society for Prosthetics and Orthotics 2013.

  8. Numerical study of radiometric forces via the direct solution of the Boltzmann kinetic equation

    NASA Astrophysics Data System (ADS)

    Anikin, Yu. A.

    2011-07-01

    The two-dimensional rarefied gas motion in a Crookes radiometer and the resulting radiometric forces are studied by numerically solving the Boltzmann kinetic equation. The collision integral is directly evaluated using a projection method, and second-order accurate TVD schemes are used to solve the advection equation. The radiometric forces are found as functions of the Knudsen number and the temperatures, and their spatial distribution is analyzed.

  9. Kinetic chain contributions to elbow function and dysfunction in sports.

    PubMed

    Ben Kibler, W; Sciascia, Aaron

    2004-10-01

    The elbow functions in throwing and other athletic activities as a link in the kinetic chain of force development, regulation, and transfer. Efficient function, with maximal performance and minimal injury risk, requires optimum activation of all the link in the kinetic chain. Injury is often associated with alterations in force production or regulation capabilities in links that may be distant to the site of injury. Evaluation of injured athletes should include screening examinations for these areas, and treatment and conditioning should also include these areas.

  10. Integration of Rooftop Photovoltaic Systems in St. Paul Ford Site's Redevelopment Plans

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Olis, D.; Mosey, G.

    The purpose of this analysis is to estimate how much electricity the redeveloped Ford Motor Company assembly plant site in St. Paul, Minnesota, might consume under different development scenarios and how much rooftop photovoltaic (PV) generation might be possible at the site. Because the current development scenarios are high-level, preliminary sketches that describe mixes of residential, retail, commercial, and industrial spaces, electricity consumption and available rooftop area for PV under each scenario can only be grossly estimated. These results are only indicative and should be used for estimating purposes only and to help inform development goals and requirements moving forward.

  11. Numerical study of a separating and reattaching flow by using Reynolds-stress tubulence closure

    NASA Technical Reports Server (NTRS)

    Amano, R. S.; Goel, P.

    1983-01-01

    The numerical study of the Reynolds-stress turbulence closure for separating, reattaching, recirculating and redeveloping flow is summarized. The calculations were made for two different closure models of pressure - strain correlation. The results were compared with the experimental data. Furthermore, these results were compared with the computations made by using the one layer and three layer treatment of k-epsilon turbulence model which were developed. Generally the computations by the Reynolds-stress model show better results than those by the k-epsilon model, in particular, some improvement was noticed in the redeveloping region of the separating and reattaching flow in a pipe with sudden expansion.

  12. Heavy metal concentrations in redeveloping soil of mine spoil under plantations of certain native woody species in dry tropical environment, India.

    PubMed

    Singh, Anand N; Zeng, De-hui; Chen, Fu-sheng

    2005-01-01

    Total concentration of heavy metals (Cd, Cr, Cu, Fe, Pb, Ni, Mn and Zn) was estimated in the redeveloping soil of mine spoil under 5-yr old plantations of four woody species namely: Albizia lebbeck, Albizia procera, Tectona grandis and Dendrocalamus strictus. The data recorded in the present study were compared with other unplanted coal mine spoil colliery, which was around to the study site and adjoining area of dry tropical forest. Among all the heavy metals, the maximum concentration was found for Fe and minimum for Cd. However, among all four species, total concentrations of these heavy metals were recorded maximally in the plantation plots of T. grandis except for Fe, while minimally in A. lebbeck except for Zn, whereas, the maximum concentration of Fe and Zn was in the plantation plots of D. strictus and A. procera. Statistical analysis revealed significant differences due to species for all the heavy metals except Cu. Among four species, A. lebbeck, A. procera and D. strictus showed more efficient for reducing heavy metal concentrations whereas T. grandis was not more effective to reduce heavy metal concentrations in redeveloping soil of mine spoil.

  13. Connecting physical and social dimensions of place attachment: What can we learn from attachment to urban recreational spaces?

    PubMed

    Madgin, Rebecca; Bradley, Lisa; Hastings, Annette

    2016-01-01

    This paper is concerned with the ways in which people form attachments to recreational spaces. More specifically it examines the relationship between recreational spaces associated with sporting activity in urban neighbourhoods and place attachment. The focus is on the ways in which changes to these spaces exposes the affective bonds between people and their surroundings. The paper applies a qualitative methodology, namely focus groups and photo elicitation, to the case study of Parkhead, a neighbourhood in the East End of Glasgow. Parkhead has historically been subjected to successive waves of redevelopment as a result of deindustrialization in the late twentieth century. More recently redevelopment associated with the 2014 Commonwealth Games involved further changes to neighbourhood recreational spaces, including refurbishing of existing sports facilities and building new ones. This paper reflects on the cumulative impacts of this redevelopment to conclude (a) that recreational sports spaces provoke multi-layered and complex attachments that are inextricably connected to both temporal and spatial narratives and (b) that research on neighbourhood recreational spaces can develop our understanding of the intricate relationship between the social and physical dimensions of place attachment.

  14. Dental students' and staff perceptions of the impact of learning environment disruption on their learning and teaching experiences.

    PubMed

    The, A J M; Adam, L; Meldrum, A; Brunton, P

    2017-10-06

    This project is a qualitative investigation into student and staff experiences of the effect of a major building redevelopment on their Dental School learning and teaching environments. Currently, there is little research exploring the impact of disruptions to the learning environment on students' learning and staff teaching experiences. Data were collected in 2016 using an online survey, semi-structured interviews and focus groups with students and staff. Data were analysed using a general inductive approach. Four broad themes emerged as follows: (i) students valued having a space for personal and collaborative work within the Dental School; (ii) both staff and students positioned staff contributions to learning experiences above the role of the physical learning environment; (iii) the majority of staff and students not feel that the physical environment limited their clinical training; and (iv) staff and students were able to adapt to the impact of building redevelopment through resilience and organisation. Results of this research have informed the provision of collegial spaces at the School, both as the building redevelopment continues, and in planning for the completed building. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  15. Biomechanical Analysis of the Closed Kinetic Chain Upper-Extremity Stability Test.

    PubMed

    Tucci, Helga T; Felicio, Lilian R; McQuade, Kevin J; Bevilaqua-Grossi, Debora; Camarini, Paula Maria Ferreira; Oliveira, Anamaria S

    2017-01-01

    The closed kinetic chain upper-extremity stability (CKCUES) test is a functional test for the upper extremity performed in the push-up position, where individuals support their body weight on 1 hand placed on the ground and swing the opposite hand until touching the hand on the ground, then switch hands and repeat the process as fast as possible for 15 s. To study scapular kinematic and kinetic measures during the CKCUES test for 3 different distances between hands. Experimental. Laboratory. 30 healthy individuals (15 male, 15 female). Participants performed 3 repetitions of the test at 3 distance conditions: original (36 in), interacromial, and 150% interacromial distance between hands. Participants completed a questionnaire on pain intensity and perceived exertion before and after the procedures. Scapular internal/external rotation, upward/downward rotation, and posterior/anterior tilting kinematics and kinetic data on maximum force and time to maximum force were measured bilaterally in all participants. Percentage of body weight on upper extremities was calculated. Data analyses were based on the total numbers of hand touches performed for each distance condition, and scapular kinematics and kinetic values were averaged over the 3 trials. Scapular kinematics, maximum force, and time to maximum force were compared for the 3 distance conditions within each gender. Significance level was set at α = .05. Scapular internal rotation, posterior tilting, and upward rotation were significantly greater in the dominant side for both genders. Scapular upward rotation was significantly greater in original distance than interacromial distance in swing phase. Time to maximum force in women was significantly greater in the dominant side. CKCUES test kinematic and kinetic measures were not different among 3 conditions based on distance between hands. However, the test might not be suitable for initial or mild-level rehabilitation due to its challenging requirements.

  16. An Analytical Model for Determining Two-Dimensional Receptor-Ligand Kinetics

    PubMed Central

    Cheung, Luthur Siu-Lun; Konstantopoulos, Konstantinos

    2011-01-01

    Cell-cell adhesive interactions play a pivotal role in major pathophysiological vascular processes, such as inflammation, infection, thrombosis, and cancer metastasis, and are regulated by hemodynamic forces generated by blood flow. Cell adhesion is mediated by the binding of receptors to ligands, which are both anchored on two-dimensional (2-D) membranes of apposing cells. Biophysical assays have been developed to determine the unstressed (no-force) 2-D affinity but fail to disclose its dependence on force. Here we develop an analytical model to estimate the 2-D kinetics of diverse receptor-ligand pairs as a function of force, including antibody-antigen, vascular selectin-ligand, and bacterial adhesin-ligand interactions. The model can account for multiple bond interactions necessary to mediate adhesion and resist detachment amid high hemodynamic forces. Using this model, we provide a generalized biophysical interpretation of the counterintuitive force-induced stabilization of cell rolling observed by a select subset of receptor-ligand pairs with specific intrinsic kinetic properties. This study enables us to understand how single-molecule and multibond biophysics modulate the macroscopic cell behavior in diverse pathophysiological processes. PMID:21575567

  17. Does Foot Anthropometry Predict Metabolic Cost During Running?

    PubMed

    van Werkhoven, Herman; Piazza, Stephen J

    2017-10-01

    Several recent investigations have linked running economy to heel length, with shorter heels being associated with less metabolic energy consumption. It has been hypothesized that shorter heels require larger plantar flexor muscle forces, thus increasing tendon energy storage and reducing metabolic cost. The goal of this study was to investigate this possible mechanism for metabolic cost reduction. Fifteen male subjects ran at 16 km⋅h -1 on a treadmill and subsequently on a force-plate instrumented runway. Measurements of oxygen consumption, kinematics, and ground reaction forces were collected. Correlational analyses were performed between oxygen consumption and anthropometric and kinetic variables associated with the ankle and foot. Correlations were also computed between kinetic variables (peak joint moment and peak tendon force) and heel length. Estimated peak Achilles tendon force normalized to body weight was found to be strongly correlated with heel length normalized to body height (r = -.751, p = .003). Neither heel length nor any other measured or calculated variable were correlated with oxygen consumption, however. Subjects with shorter heels experienced larger Achilles tendon forces, but these forces were not associated with reduced metabolic cost. No other anthropometric and kinetic variables considered explained the variance in metabolic cost across individuals.

  18. Variability of Grip Kinetics during Adult Signature Writing

    PubMed Central

    Ghali, Bassma; Thalanki Anantha, Nayanashri; Chan, Jennifer; Chau, Tom

    2013-01-01

    Grip kinetics and their variation are emerging as important considerations in the clinical assessment of handwriting pathologies, fine motor rehabilitation, biometrics, forensics and ergonomic pen design. This study evaluated the intra- and inter-participant variability of grip shape kinetics in adults during signature writing. Twenty (20) adult participants wrote on a digitizing tablet using an instrumented pen that measured the forces exerted on its barrel. Signature samples were collected over 10 days, 3 times a day, to capture temporal variations in grip shape kinetics. A kinetic topography (i.e., grip shape image) was derived per signature by time-averaging the measured force at each of 32 locations around the pen barrel. The normalized cross correlations (NCC) of grip shape images were calculated within- and between-participants. Several classification algorithms were implemented to gauge the error rate of participant discrimination based on grip shape kinetics. Four different grip shapes emerged and several participants made grip adjustments (change in grip shape or grip height) or rotated the pen during writing. Nonetheless, intra-participant variation in grip kinetics was generally much smaller than inter-participant force variations. Using the entire grip shape images as a 32-dimensional input feature vector, a K-nearest neighbor classifier achieved an error rate of % in discriminating among participants. These results indicate that writers had unique grip shape kinetics that were repeatable over time but distinct from those of other participants. The topographic analysis of grip kinetics may inform the development of personalized interventions or customizable grips in clinical and industrial applications, respectively. PMID:23658812

  19. Variability of grip kinetics during adult signature writing.

    PubMed

    Ghali, Bassma; Thalanki Anantha, Nayanashri; Chan, Jennifer; Chau, Tom

    2013-01-01

    Grip kinetics and their variation are emerging as important considerations in the clinical assessment of handwriting pathologies, fine motor rehabilitation, biometrics, forensics and ergonomic pen design. This study evaluated the intra- and inter-participant variability of grip shape kinetics in adults during signature writing. Twenty (20) adult participants wrote on a digitizing tablet using an instrumented pen that measured the forces exerted on its barrel. Signature samples were collected over 10 days, 3 times a day, to capture temporal variations in grip shape kinetics. A kinetic topography (i.e., grip shape image) was derived per signature by time-averaging the measured force at each of 32 locations around the pen barrel. The normalized cross correlations (NCC) of grip shape images were calculated within- and between-participants. Several classification algorithms were implemented to gauge the error rate of participant discrimination based on grip shape kinetics. Four different grip shapes emerged and several participants made grip adjustments (change in grip shape or grip height) or rotated the pen during writing. Nonetheless, intra-participant variation in grip kinetics was generally much smaller than inter-participant force variations. Using the entire grip shape images as a 32-dimensional input feature vector, a K-nearest neighbor classifier achieved an error rate of 1.2±0.4% in discriminating among participants. These results indicate that writers had unique grip shape kinetics that were repeatable over time but distinct from those of other participants. The topographic analysis of grip kinetics may inform the development of personalized interventions or customizable grips in clinical and industrial applications, respectively.

  20. Dependence of cross-bridge kinetics on myosin light chain isoforms in rabbit and rat skeletal muscle fibres.

    PubMed

    Andruchov, Oleg; Andruchova, Olena; Wang, Yishu; Galler, Stefan

    2006-02-15

    Cross-bridge kinetics underlying stretch-induced force transients was studied in fibres with different myosin light chain (MLC) isoforms from skeletal muscles of rabbit and rat. The force transients were induced by stepwise stretches (< 0.3% of fibre length) applied on maximally Ca2+-activated skinned fibres. Fast fibre types IIB, IID (or IIX) and IIA and the slow fibre type I containing the myosin heavy chain isoforms MHC-IIb, MHC-IId (or MHC-IIx), MHC-IIa and MHC-I, respectively, were investigated. The MLC isoform content varied within fibre types. Fast fibre types contained the fast regulatory MLC isoform MLC2f and different proportions of the fast alkali MLC isoforms MLC1f and MLC3f. Type I fibres contained the slow regulatory MLC isoform MLC2s and the slow alkali MLC isoform MLC1s. Slow MLC isoforms were also present in several type IIA fibres. The kinetics of force transients differed by a factor of about 30 between fibre types (order from fastest to slowest kinetics: IIB > IID > IIA > I). The kinetics of the force transients was not dependent on the relative content of MLC1f and MLC3f. Type IIA fibres containing fast and slow MLC isoforms were about 1.2 times slower than type IIA fibres containing only fast MLC isoforms. We conclude that while the cross-bridge kinetics is mainly determined by the MHC isoforms present, it is affected by fast and slow MLC isoforms but not by the relative content of MLC1f and MLC3f. Thus, the physiological role of fast and slow MLC isoforms in type IIA fibres is a fine-tuning of the cross-bridge kinetics.

  1. Friction phenomena and phase transition in the underdamped two-dimensional Frenkel-Kontorova model

    NASA Astrophysics Data System (ADS)

    Yang, Yang; Duan, Wen-Shan; Chen, Jian-Min; Yang, Lei; Tekić, Jasmina; Shao, Zhi-Gang; Wang, Cang-Long

    2010-11-01

    Locked-to-sliding phase transition has been studied in the driven two-dimensional Frenkel-Kontorova model with the square symmetric substrate potential. It is found that as the driving force increases, the system transfers from the locked state to the sliding state where the motion of particles is in the direction different from that of driving force. With the further increase in driving force, at some critical value, the particles start to move in the direction of driving force. These two critical forces, the static friction or depinning force, and the kinetic friction force for which particles move in the direction of driving force have been analyzed for different system parameters. Different scenarios of phase transitions have been examined and dynamical phases are classified. In the case of zero misfit angle, the analytical expressions for static and kinetic friction force have been obtained.

  2. A single-molecule force spectroscopy study of the interactions between lectins and carbohydrates on cancer and normal cells

    NASA Astrophysics Data System (ADS)

    Zhao, Weidong; Cai, Mingjun; Xu, Haijiao; Jiang, Junguang; Wang, Hongda

    2013-03-01

    The interaction forces between carbohydrates and lectins were investigated by single-molecule force spectroscopy on both cancer and normal cells. The binding kinetics was also studied, which shows that the carbohydrate-lectin complex on cancer cells is less stable than that on normal cells.The interaction forces between carbohydrates and lectins were investigated by single-molecule force spectroscopy on both cancer and normal cells. The binding kinetics was also studied, which shows that the carbohydrate-lectin complex on cancer cells is less stable than that on normal cells. Electronic supplementary information (ESI) available: Experimental details. See DOI: 10.1039/c3nr00553d

  3. Injury tolerance criteria for short-duration axial impulse loading of the isolated tibia.

    PubMed

    Quenneville, Cheryl E; McLachlin, Stewart D; Greeley, Gillian S; Dunning, Cynthia E

    2011-01-01

    Impulse loading of the lower leg during events such as ejection seat landings or in-vehicle land mine blasts may result in devastating injuries. These impacts achieve higher forces over shorter durations than car crashes, from which experimental results have formed the current basis for protective measures of an axial force limit of 5.4 kN, as registered by an anthropomorphic test device (ATD). The hypotheses of this study were that the injury tolerance of the isolated tibia to short-duration axial loading is higher than that previously reported and that secondary parameters such as momentum or kinetic energy are significant for fracture tolerance, in addition to force. Seven pairs of cadaveric tibias were impacted using a pneumatic testing apparatus, replicating short-duration axial impulse events. One specimen from each pair was impacted with a light mass and the contralateral impacted with a heavy mass, to investigate the effects of momentum and kinetic energy, as well as force, on injury. Impacts were applied incrementally until failure. Force, kinetic energy, age, and height were shown to be significant factors in the probability of fracture. A 10% risk of injury corresponded to an impact force of 7.9 kN, with an average kinetic energy of 240 J. In comparison, this same impact level applied to an ATD would register a force of 16.2 kN because of the higher stiffness of the ATD. These results suggest that the current injury standard may be too conservative for the tibia during high-speed impacts such as in-vehicle land mine blasts and that factors in addition to force should be taken into consideration.

  4. Determination of thermodynamics and kinetics of RNA reactions by force

    PubMed Central

    Tinoco, Ignacio; Li, Pan T. X.; Bustamante, Carlos

    2008-01-01

    Single-molecule methods have made it possible to apply force to an individual RNA molecule. Two beads are attached to the RNA; one is on a micropipette, the other is in a laser trap. The force on the RNA and the distance between the beads are measured. Force can change the equilibrium and the rate of any reaction in which the product has a different extension from the reactant. This review describes use of laser tweezers to measure thermodynamics and kinetics of unfolding/refolding RNA. For a reversible reaction the work directly provides the free energy; for irreversible reactions the free energy is obtained from the distribution of work values. The rate constants for the folding and unfolding reactions can be measured by several methods. The effect of pulling rate on the distribution of force-unfolding values leads to rate constants for unfolding. Hopping of the RNA between folded and unfolded states at constant force provides both unfolding and folding rates. Force-jumps and force-drops, similar to the temperature jump method, provide direct measurement of reaction rates over a wide range of forces. The advantages of applying force and using single-molecule methods are discussed. These methods, for example, allow reactions to be studied in non-denaturing solvents at physiological temperatures; they also simplify analysis of kinetic mechanisms because only one intermediate at a time is present. Unfolding of RNA in biological cells by helicases, or ribosomes, has similarities to unfolding by force. PMID:17040613

  5. k-OptForce: Integrating Kinetics with Flux Balance Analysis for Strain Design

    PubMed Central

    Chowdhury, Anupam; Zomorrodi, Ali R.; Maranas, Costas D.

    2014-01-01

    Computational strain design protocols aim at the system-wide identification of intervention strategies for the enhanced production of biochemicals in microorganisms. Existing approaches relying solely on stoichiometry and rudimentary constraint-based regulation overlook the effects of metabolite concentrations and substrate-level enzyme regulation while identifying metabolic interventions. In this paper, we introduce k-OptForce, which integrates the available kinetic descriptions of metabolic steps with stoichiometric models to sharpen the prediction of intervention strategies for improving the bio-production of a chemical of interest. It enables identification of a minimal set of interventions comprised of both enzymatic parameter changes (for reactions with available kinetics) and reaction flux changes (for reactions with only stoichiometric information). Application of k-OptForce to the overproduction of L-serine in E. coli and triacetic acid lactone (TAL) in S. cerevisiae revealed that the identified interventions tend to cause less dramatic rearrangements of the flux distribution so as not to violate concentration bounds. In some cases the incorporation of kinetic information leads to the need for additional interventions as kinetic expressions render stoichiometry-only derived interventions infeasible by violating concentration bounds, whereas in other cases the kinetic expressions impart flux changes that favor the overproduction of the target product thereby requiring fewer direct interventions. A sensitivity analysis on metabolite concentrations shows that the required number of interventions can be significantly affected by changing the imposed bounds on metabolite concentrations. Furthermore, k-OptForce was capable of finding non-intuitive interventions aiming at alleviating the substrate-level inhibition of key enzymes in order to enhance the flux towards the product of interest, which cannot be captured by stoichiometry-alone analysis. This study paves the way for the integrated analysis of kinetic and stoichiometric models and enables elucidating system-wide metabolic interventions while capturing regulatory and kinetic effects. PMID:24586136

  6. Choose Wisely: Static or Kinetic Friction--The Power of Dimensionless Plots

    ERIC Educational Resources Information Center

    Ludwigsen, Daniel; Svinarich, Kathryn

    2009-01-01

    Consider a problem of sliding blocks, one stacked atop the other, resting on a frictionless table. If the bottom block is pulled horizontally, nature makes a choice: if the applied force is small, static friction between the blocks accelerates the blocks together, but with a large force the blocks slide apart. In that case, kinetic friction still…

  7. Microgravity

    NASA Image and Video Library

    2001-06-06

    Atomic force microscopy uses laser technology to reveal a defect, a double-screw dislocation, on the surface of this crystal of canavalin, a major source of dietary protein for humans and domestic animals. When a crystal grows, attachment kinetics and transport kinetics are competing for control of the molecules. As a molecule gets close to the crystal surface, it has to attach properly for the crystal to be usable. NASA has funded investigators to look at those attachment kinetics from a theoretical standpoint and an experimental standpoint. Dr. Alex McPherson of the University of California, Irvine, is one of those investigators. He uses X-ray diffraction and atomic force microscopy in his laboratory to answer some of the many questions about how protein crystals grow. Atomic force microscopy provides a means of looking at how individual molecules are added to the surface of growing protein crystals. This helps McPherson understand the kinetics of protein crystal growth. McPherson asks, How fast do crystals grow? What are the forces involved? Investigators funded by NASA have clearly shown that such factors as the level of supersaturation and the rate of growth all affect the habit [characteristic arrangement of facets] of the crystal and the defects that occur in the crystal.

  8. Protein crystal growth

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Atomic force microscopy uses laser technology to reveal a defect, a double-screw dislocation, on the surface of this crystal of canavalin, a major source of dietary protein for humans and domestic animals. When a crystal grows, attachment kinetics and transport kinetics are competing for control of the molecules. As a molecule gets close to the crystal surface, it has to attach properly for the crystal to be usable. NASA has funded investigators to look at those attachment kinetics from a theoretical standpoint and an experimental standpoint. Dr. Alex McPherson of the University of California, Irvine, is one of those investigators. He uses X-ray diffraction and atomic force microscopy in his laboratory to answer some of the many questions about how protein crystals grow. Atomic force microscopy provides a means of looking at how individual molecules are added to the surface of growing protein crystals. This helps McPherson understand the kinetics of protein crystal growth. McPherson asks, How fast do crystals grow? What are the forces involved? Investigators funded by NASA have clearly shown that such factors as the level of supersaturation and the rate of growth all affect the habit [characteristic arrangement of facets] of the crystal and the defects that occur in the crystal.

  9. Changing Sagittal-Plane Landing Styles to Modulate Impact and Tibiofemoral Force Magnitude and Directions Relative to the Tibia

    PubMed Central

    Shimokochi, Yohei; Ambegaonkar, Jatin P.; Meyer, Eric G.

    2016-01-01

    Context: Ground reaction force (GRF) and tibiofemoral force magnitudes and directions have been shown to affect anterior cruciate ligament loading during landing. However, the kinematic and kinetic factors modifying these 2 forces during landing are unknown. Objective: To clarify the intersegmental kinematic and kinetic links underlying the alteration of the GRF and tibiofemoral force vectors secondary to changes in the sagittal-plane body position during single-legged landing. Design: Crossover study. Setting: Laboratory. Patients or Other Participants: Twenty recreationally active participants (age = 23.4 ± 3.6 years, height = 171.0 ± 9.4 cm, mass = 73.3 ± 12.7 kg). Intervention(s): Participants performed single-legged landings using 3 landing styles: self-selected landing (SSL), body leaning forward and landing on the toes (LFL), and body upright with flat-footed landing (URL). Three-dimensional kinetics and kinematics were recorded. Main Outcome Measure(s): Sagittal-plane tibial inclination and knee-flexion angles, GRF magnitude and inclination angles relative to the tibia, and proximal tibial forces at peak tibial axial forces. Results: The URL resulted in less time to peak tibial axial forces, smaller knee-flexion angles, and greater magnitude and a more anteriorly inclined GRF vector relative to the tibia than did the SSL. These changes led to the greatest peak tibial axial and anterior shear forces in the URL among the 3 landing styles. Conversely, the LFL resulted in longer time to peak tibial axial forces, greater knee-flexion angles, and reduced magnitude and a more posteriorly inclined GRF vector relative to the tibia than the SSL. These changes in LFL resulted in the lowest peak tibial axial and largest posterior shear forces among the 3 landing styles. Conclusions: Sagittal-plane intersegmental kinematic and kinetic links strongly affected the magnitude and direction of GRF and tibiofemoral forces during the impact phase of single-legged landing. Therefore, improving sagittal-plane landing mechanics is important in reducing harmful magnitudes and directions of impact forces on the anterior cruciate ligament. PMID:27723362

  10. Designing sustainable and economically attractive brownfield revitalization options using an integrated assessment model.

    PubMed

    Schädler, S; Morio, M; Bartke, S; Rohr-Zänker, R; Finkel, M

    2011-03-01

    We describe the development of an integrated assessment model which evaluates redevelopment options of large contaminated brownfields and we present the application of the model in a case study. Aiming to support efficient and sustainable revitalization and communication between stakeholders, the presented assessment model integrates three pinnacles of brownfield revitalization: (i) subsurface remediation and site preparation costs, (ii) market-oriented economic appraisal, and (iii) the expected contribution of planned future land use to sustainable community and regional development. For the assessment, focus is set on the early stage of the brownfield redevelopment process, which is characterized by limited data availability and by flexibility in land use planning and development scope. At this stage, revealing the consequences of adjustments and alterations in planning options can foster efficiency in communication between the involved parties and thereby facilitates the brownfield revitalization process. Results from the case-study application indicate that the integrated assessment provides help in the identification of land use options beneficial in both a sustainable and an economical sense. For the study site it is shown on one hand that brownfield redevelopment is not automatically in line with sustainable regional development, and on the other hand it is demonstrated that additional contributions to sustainability are not intrinsically tied to increased costs. Copyright © 2010 Elsevier Ltd. All rights reserved.

  11. Integrated planning and spatial evaluation of megasite remediation and reuse options

    NASA Astrophysics Data System (ADS)

    Schädler, Sebastian; Morio, Maximilian; Bartke, Stephan; Finkel, Michael

    2012-01-01

    Redevelopment of large contaminated brownfields (megasites) is often hampered by a lack of communication and harmonization among diverse stakeholders with potentially conflicting interests. Decision support is required to provide integrative yet transparent evaluation of often complex spatial information to stakeholders with different areas of expertise. It is considered crucial for successful redevelopment to identify a shared vision of how the respective contaminated site could be remediated and redeveloped. We describe a framework of assessment methods and models that analyzes and visualizes site- and land use-specific spatial information at the screening level, with the aim to support the derivation of recommendable land use layouts and to initiate further and more detailed planning. The framework integrates a GIS-based identification of areas to be remediated, an estimation of associated clean-up costs, a spatially explicit market value appraisal, and an assessment of the planned future land use's contribution to sustainable urban and regional development. Case study results show that derived options are potentially favorable in both a sustainability and an economic sense and that iterative re-planning is facilitated by the evaluation and visualization of economic, ecological and socio-economic aspects. The framework supports an efficient early judgment about whether and how abandoned land may be assigned a sustainable and marketable land use.

  12. Using risk maps to link land value damage and risk as basis of flexible risk management for brownfield redevelopment.

    PubMed

    Chen, I-chun; Ma, Hwong-wen

    2013-02-01

    Brownfield redevelopment involves numerous uncertain financial risks associated with market demand and land value. To reduce the uncertainty of the specific impact of land value and social costs, this study develops small-scale risk maps to determine the relationship between population risk (PR) and damaged land value (DLV) to facilitate flexible land reutilisation plans. This study used the spatial variability of exposure parameters in each village to develop the contaminated site-specific risk maps. In view of the combination of risk and cost, risk level that most affected land use was mainly 1.00×10(-6) to 1.00×10(-5) in this study area. Village 2 showed the potential for cost-effective conversion with contaminated land development. If the risk of remediation target was set at 5.00×10(-6), the DLV could be reduced by NT$15,005 million for the land developer. The land developer will consider the net benefit by quantifying the trade-off between the changes of land value and the cost of human health. In this study, small-scale risk maps can illuminate the economic incentive potential for contaminated site redevelopment through the adjustment of land value damage and human health risk. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Increased cross-bridge recruitment contributes to transient increase in force generation beyond maximal capacity in human myocardium.

    PubMed

    Milani-Nejad, Nima; Chung, Jae-Hoon; Canan, Benjamin D; Fedorov, Vadim V; Whitson, Bryan A; Kilic, Ahmet; Mohler, Peter J; Janssen, Paul M L

    2018-01-01

    Cross-bridge attachment allows force generation to occur, and rate of tension redevelopment (k tr ) is a commonly used index of cross-bridge cycling rate. Tension overshoots have been observed briefly after a slack-restretch k tr maneuver in various species of animal models and humans. In this study, we set out to determine the properties of these overshoots and their possible underlying mechanism. Utilizing human cardiac trabeculae, we have found that tension overshoots are temperature-dependent and that they do not occur at resting states. In addition, we have found that myosin cross-bridge cycle is vital to these overshoots as inhibition of the cycle results in the blunting of the overshoots and the magnitude of the overshoots are dependent on the level of myofilament activation. Lastly, we show that the number of cross-bridges transiently increase during tension overshoots. These findings lead us to conclude that tension overshoots are likely due to a transient enhancement of the recruitment of myosin heads into the cross-bridge cycling, regulated by the myocardium, and with potential physiological significance in determining cardiac output. We show that isolated human myocardium is capable of transiently increasing its maximal force generation capability by increasing cross-bridge recruitment following slack-restretch maneuver. This process can potentially have important implications and significance in cardiac contraction in vivo. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Estimation of foot joint kinetics in three and four segment foot models using an existing proportionality scheme: Application in paediatric barefoot walking.

    PubMed

    Deschamps, Kevin; Eerdekens, Maarten; Desmet, Dirk; Matricali, Giovanni Arnoldo; Wuite, Sander; Staes, Filip

    2017-08-16

    Recent studies which estimated foot segment kinetic patterns were found to have inconclusive data on one hand, and did not dissociate the kinetics of the chopart and lisfranc joint. The current study aimed therefore at reproducing independent, recently published three-segment foot kinetic data (Study 1) and in a second stage expand the estimation towards a four-segment model (Study 2). Concerning the reproducibility study, two recently published three segment foot models (Bruening et al., 2014; Saraswat et al., 2014) were reproduced and kinetic parameters were incorporated in order to calculate joint moments and powers of paediatric cohorts during gait. Ground reaction forces were measured with an integrated force/pressure plate measurement set-up and a recently published proportionality scheme was applied to determine subarea total ground reaction forces. Regarding Study 2, moments and powers were estimated with respect to the Instituto Ortopedico Rizzoli four-segment model. The proportionality scheme was expanded in this study and the impact of joint centre location on kinetic data was evaluated. Findings related to Study 1 showed in general good agreement with the kinetic data published by Bruening et al. (2014). Contrarily, the peak ankle, midfoot and hallux powers published by Saraswat et al. (2014) are disputed. Findings of Study 2 revealed that the chopart joint encompasses both power absorption and generation, whereas the Lisfranc joint mainly contributes to power generation. The results highlights the necessity for further studies in the field of foot kinetic models and provides a first estimation of the kinetic behaviour of the Lisfranc joint. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Allocating risk capital for a brownfields redevelopment project under hydrogeological and financial uncertainty.

    PubMed

    Yu, Soonyoung; Unger, Andre J A; Parker, Beth; Kim, Taehee

    2012-06-15

    In this study, we defined risk capital as the contingency fee or insurance premium that a brownfields redeveloper needs to set aside from the sale of each house in case they need to repurchase it at a later date because the indoor air has been detrimentally affected by subsurface contamination. The likelihood that indoor air concentrations will exceed a regulatory level subject to subsurface heterogeneity and source zone location uncertainty is simulated by a physics-based hydrogeological model using Monte Carlo realizations, yielding the probability of failure. The cost of failure is the future value of the house indexed to the stochastic US National Housing index. The risk capital is essentially the probability of failure times the cost of failure with a surcharge to compensate the developer against hydrogeological and financial uncertainty, with the surcharge acting as safety loading reflecting the developers' level of risk aversion. We review five methodologies taken from the actuarial and financial literature to price the risk capital for a highly stylized brownfield redevelopment project, with each method specifically adapted to accommodate our notion of the probability of failure. The objective of this paper is to develop an actuarially consistent approach for combining the hydrogeological and financial uncertainty into a contingency fee that the brownfields developer should reserve (i.e. the risk capital) in order to hedge their risk exposure during the project. Results indicate that the price of the risk capital is much more sensitive to hydrogeological rather than financial uncertainty. We use the Capital Asset Pricing Model to estimate the risk-adjusted discount rate to depreciate all costs to present value for the brownfield redevelopment project. A key outcome of this work is that the presentation of our risk capital valuation methodology is sufficiently generalized for application to a wide variety of engineering projects. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. Refolding dynamics of stretched biopolymers upon force quench

    PubMed Central

    Hyeon, Changbong; Morrison, Greg; Pincus, David L.; Thirumalai, D.

    2009-01-01

    Single-molecule force spectroscopy methods can be used to generate folding trajectories of biopolymers from arbitrary regions of the folding landscape. We illustrate the complexity of the folding kinetics and generic aspects of the collapse of RNA and proteins upon force quench by using simulations of an RNA hairpin and theory based on the de Gennes model for homopolymer collapse. The folding time, τF, depends asymmetrically on δfS = f S − f m and δf Q = f m − f Q where f S (f Q) is the stretch (quench) force and f m is the transition midforce of the RNA hairpin. In accord with experiments, the relaxation kinetics of the molecular extension, R(t), occurs in three stages: A rapid initial decrease in the extension is followed by a plateau and finally, an abrupt reduction in R(t) occurs as the native state is approached. The duration of the plateau increases as λ = τ Q/τ F decreases (where τ Q is the time in which the force is reduced from f S to f Q). Variations in the mechanisms of force-quench relaxation as λ is altered are reflected in the experimentally measurable time-dependent entropy, which is computed directly from the folding trajectories. An analytical solution of the de Gennes model under tension reproduces the multistage stage kinetics in R(t). The prediction that the initial stages of collapse should also be a generic feature of polymers is validated by simulation of the kinetics of toroid (globule) formation in semiflexible (flexible) homopolymers in poor solvents upon quenching the force from a fully stretched state. Our findings give a unified explanation for multiple disparate experimental observations of protein folding. PMID:19915145

  17. Kinetics of cross-slope running.

    PubMed

    Willwacher, Steffen; Fischer, Katina Mira; Benker, Rita; Dill, Stephan; Brüggemann, Gert-Peter

    2013-11-15

    The purpose of the present study was to identify kinetic responses to running on mediolaterally elevated (cross-sloped) running surfaces. Ground reaction forces (GRFs), GRF lever arms and joint moment characteristics of 19 male runners were analyzed when running at 3.5m/s on a custom-made, tiltable runway. Tilt angles of 3° and 6° for medial and lateral elevation were analyzed using a 10 camera Vicon Nexus system and a force platform. The point of force application of the GRF showed a systematic shift in the order of 1-1.5cm to either the lateral or medial aspect of the foot for lateral or medial inclinations, respectively. Consequently, the strongest significant effects of tilt orientation and level on joint kinetics and ground reaction force lever arms were identified at the ankle, knee and hip joint in the frontal plane of movement. External eversion moments at the ankle were significantly increased by 35% for 6° of lateral elevation and decreased by 16% for 6° of medial elevation. Altering the cross-slope of the running surface changed the pattern of ankle joint moments in the transversal plane. Effect sizes were on average larger for laterally elevated conditions, indicating a higher sensitivity of kinetic parameters to this kind of surface tilt. These alterations in joint kinetics should be considered in the choice of the running environment, especially for specific risk groups, like runners in rehabilitation processes. © 2013 Elsevier Ltd. All rights reserved.

  18. Realistic kinetic loading of the jaw system during single chewing cycles: a finite element study.

    PubMed

    Martinez Choy, S E; Lenz, J; Schweizerhof, K; Schmitter, M; Schindler, H J

    2017-05-01

    Although knowledge of short-range kinetic interactions between antagonistic teeth during mastication is of essential importance for ensuring interference-free fixed dental reconstructions, little information is available. In this study, the forces on and displacements of the teeth during kinetic molar biting simulating the power stroke of a chewing cycle were investigated by use of a finite-element model that included all the essential components of the human masticatory system, including an elastic food bolus. We hypothesised that the model can approximate the loading characteristics of the dentition found in previous experimental studies. The simulation was a transient analysis, that is, it considered the dynamic behaviour of the jaw. In particular, the reaction forces on the teeth and joints arose from contact, rather than nodal forces or constraints. To compute displacements of the teeth, the periodontal ligament (PDL) was modelled by use of an Ogden material model calibrated on the basis of results obtained in previous experiments. During the initial holding phase of the power stroke, bite forces were aligned with the roots of the molars until substantial deformation of the bolus occurred. The forces tilted the molars in the bucco-lingual and mesio-distal directions, but as the intrusive force increased the teeth returned to their initial configuration. The Ogden material model used for the PDL enabled accurate prediction of the displacements observed in experimental tests. In conclusion, the comprehensive kinetic finite element model reproduced the kinematic and loading characteristics of previous experimental investigations. © 2017 John Wiley & Sons Ltd.

  19. Viscous interaction of flow redevelopment after flow reattachment with supersonic external streams

    NASA Technical Reports Server (NTRS)

    Chow, W. L.; Spring, D. J.

    1975-01-01

    A flow model has been developed to study the flow development after reattachment with supersonic external streams. Special attention is given to the pressure difference across the viscous layer, and it is suggested that such a flow redevelopment can be treated as a relaxation of this pressure difference. Upon correlating the pressure difference with a slope parameter of the velocity profile, the system of equations governing the flow would produce a saddle point singularity corresponding to the fully rehabilitated asymptotic flow condition. A method of calculation for this flowfield, in conjunction with the matching of the upstream flow, has been derived and is discussed. Samples of calculations are also presented. Reasonably good agreement with experimental data has also been observed.

  20. Determination of outer layer and bulk dehydration kinetics of trehalose dihydrate using atomic force microscopy, gravimetric vapour sorption and near infrared spectroscopy.

    PubMed

    Jones, Matthew D; Beezer, Anthony E; Buckton, Graham

    2008-10-01

    Knowledge of the kinetics of solid state reactions is important when considering the stability of many medicines. Potentially, such reactions could follow different kinetics on the surface of particles when compared with their interior, yet solid state processes are routinely followed using only bulk characterisation techniques. Atomic force microscopy (AFM) has previously been shown to be a suitable technique for the investigation of surface processes, but has not been combined with bulk techniques in order to analyse surface and bulk kinetics separately. This report therefore describes the investigation of the outer layer and bulk kinetics of the dehydration of trehalose dihydrate at ambient temperature and low humidity, using AFM, dynamic vapour sorption (DVS) and near infrared spectroscopy (NIR). The use of AFM enabled the dehydration kinetics of the outer layers to be determined both directly and from bulk data. There were no significant differences between the outer layer dehydration kinetics determined using these methods. AFM also enabled the bulk-only kinetics to be analysed from the DVS and NIR data. These results suggest that the combination of AFM and bulk characterisation techniques should enable a more complete understanding of the kinetics of certain solid state reactions to be achieved. (c) 2008 Wiley-Liss, Inc. and the American Pharmacists Association

  1. Arbitrary amplitude dust kinetic Alfvén solitary waves in the presence of polarization force

    NASA Astrophysics Data System (ADS)

    Singh, Manpreet; Kaur, Nimardeep; Saini, N. S.

    2018-02-01

    In this investigation, the effect of polarization force on dust kinetic Alfvén solitary waves (DKASWs) in a magnetized dusty plasma consisting of dust fluid, electrons, and positively charged ions is studied. By incorporating density non-uniformity and polarization force in the fluid model equations, the energy balance equation is derived, and from the expression for Sagdeev pseudopotential, the existence conditions for solitary structures in terms of Mach number are determined. From the numerical analysis of Sagdeev pseudopotential, compressive and rarefactive DKASWs at sub- and super-Alfvénic speeds are observed. These waves are significantly affected by varying polarization force, angle of propagation, plasma beta, and Mach number.

  2. Magnetic Fluctuation-Driven Intrinsic Flow in a Toroidal Plasma

    NASA Astrophysics Data System (ADS)

    Brower, D. L.; Ding, W. X.; Lin, L.; Almagri, A. F.; den Hartog, D. J.; Sarff, J. S.

    2012-10-01

    Magnetic fluctuations have been long observed in various magnetic confinement configurations. These perturbations may arise naturally from plasma instabilities such as tearing modes and energetic particle driven modes, but they can also be externally imposed by error fields or external magnetic coils. It is commonly observed that large MHD modes lead to plasma locking (no rotation) due to torque produced by eddy currents on the wall, and it is predicted that stochastic field induces flow damping where the radial electric field is reduced. Flow generation is of great importance to fusion plasma research, especially low-torque devices like ITER, as it can act to improve performance. Here we describe new measurements in the MST reversed field pinch (RFP) showing that the coherent interaction of magnetic and particle density fluctuations can produce a turbulent fluctuation-induced kinetic force, which acts to drive intrinsic plasma rotation. Key observations include; (1) the average kinetic force resulting from density fluctuations, ˜ 0.5 N/m^3, is comparable to the intrinsic flow acceleration, and (2) between sawtooth crashes, the spatial distribution of the kinetic force is directed to create a sheared parallel flow profile that is consistent with the measured flow profile in direction and amplitude, suggesting the kinetic force is responsible for intrinsic plasma rotation.

  3. Brownfields

    EPA Pesticide Factsheets

    The Brownfields site provides information about brownfields sites and cleanup and reuse challenges and liabilities. It will showcase activities and accomplishments and provide info on funding opportunities and collaboration in brownfields redevelopment.

  4. Forced unfolding of single-chain polymeric nanoparticles.

    PubMed

    Hosono, Nobuhiko; Kushner, Aaron M; Chung, Jaeyoon; Palmans, Anja R A; Guan, Zhibin; Meijer, E W

    2015-06-03

    Atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) is applied to single-chain polymeric nanoparticles (SCPNs) to acquire information about the internal folding structure of SCPNs and inherent kinetic parameters of supramolecular self-assembling motifs embedded into the SCPNs. The SCPNs used here are polyacrylate-based polymers carrying 2-ureido-4-[1H]-pyrimidinone (UPy) or benzene-1,3,5-tricarboxamide (BTA) pendants that induce an intramolecular chain collapse into nanoparticles consisting of one polymer chain only via internal supramolecular cross-linking. The SCPN is stretched by an AFM cantilever to unfold mechanically, which allows measuring of force-extension profiles of the SCPNs. Consecutive peaks observed in the force profiles are attributed to rupture events of self-assembled UPy/BTA units in the SCPNs. The force profiles have been analyzed statistically for a series of polymers with different UPy/BTA incorporation densities. The results provide insights into the internal conformation of SCPNs, where the folding structure can be changed with the incorporation density of UPy/BTA. In addition, dynamic loading rate analysis allows the determination of kinetic parameters of BTA self-assembly, which has not been accessible by any other method. This study offers a rational tool for understanding the folding structure, kinetics, and pathway of two series of SCPNs.

  5. Dynamo action in dissipative, forced, rotating MHD turbulence

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shebalin, John V.

    2016-06-15

    Magnetohydrodynamic (MHD) turbulence is an inherent feature of large-scale, energetic astrophysical and geophysical magnetofluids. In general, these are rotating and are energized through buoyancy and shear, while viscosity and resistivity provide a means of dissipation of kinetic and magnetic energy. Studies of unforced, rotating, ideal (i.e., non-dissipative) MHD turbulence have produced interesting results, but it is important to determine how these results are affected by dissipation and forcing. Here, we extend our previous work and examine dissipative, forced, and rotating MHD turbulence. Incompressibility is assumed, and finite Fourier series represent turbulent velocity and magnetic field on a 64{sup 3} grid.more » Forcing occurs at an intermediate wave number by a method that keeps total energy relatively constant and allows for injection of kinetic and magnetic helicity. We find that 3-D energy spectra are asymmetric when forcing is present. We also find that dynamo action occurs when forcing has either kinetic or magnetic helicity, with magnetic helicity injection being more important. In forced, dissipative MHD turbulence, the dynamo manifests itself as a large-scale coherent structure that is similar to that seen in the ideal case. These results imply that MHD turbulence, per se, may play a fundamental role in the creation and maintenance of large-scale (i.e., dipolar) stellar and planetary magnetic fields.« less

  6. Brownfields Projects in EPA's Pacific Southwest

    EPA Pesticide Factsheets

    Project fact sheet focusing on brownfields-funding that has helped the redevelopment of unused, contaminated sites into usable, public areas - especially in underserved, overburdened and economically distressed locations.

  7. Kinetics of the Multistep Rupture of Fibrin ‘A-a’ Polymerization Interactions Measured Using Atomic Force Microscopy

    PubMed Central

    Averett, Laurel E.; Schoenfisch, Mark H.; Akhremitchev, Boris B.; Gorkun, Oleg V.

    2009-01-01

    Abstract Fibrin, the structural scaffold of blood clots, spontaneously polymerizes through the formation of ‘A-a’ knob-hole bonds. When subjected to external force, the dissociation of this bond is accompanied by two to four abrupt changes in molecular dimension observable as rupture events in a force curve. Herein, the configuration, molecular extension, and kinetic parameters of each rupture event are examined. The increases in contour length indicate that the D region of fibrinogen can lengthen by ∼50% of the length of a fibrin monomer before rupture of the ‘A-a’ interaction. The dependence of the dissociation rate on applied force was obtained using probability distributions of rupture forces collected at different pull-off velocities. These distributions were fit using a model in which the effects of the shape of the binding potential are used to quantify the kinetic parameters of forced dissociation. We found that the weak initial rupture (i.e., event 1) was not well approximated by these models. The ruptured bonds comprising the strongest ruptures, events 2 and 3, had kinetic parameters similar to those commonly found for the mechanical unfolding of globular proteins. The bonds ruptured in event 4 were well described by these analyses, but were more loosely bound than the bonds in events 2 and 3. We propose that the first event represents the rupture of an unknown interaction parallel to the ‘A-a’ bond, events 2 and 3 represent unfolding of structures in the D region of fibrinogen, and event 4 is the rupture of the ‘A-a’ knob-hole bond weakened by prior structural unfolding. Comparison of the activation energy obtained via force spectroscopy measurements with the thermodynamic free energy of ‘A-a’ bond dissociation indicates that the ‘A-a’ bond may be more resistant to rupture by applied force than to rupture by thermal dissociation. PMID:19917237

  8. Activation Dependence of Stretch Activation in Mouse Skinned Myocardium: Implications for Ventricular Function

    PubMed Central

    Stelzer, Julian E.; Larsson, Lars; Fitzsimons, Daniel P.; Moss, Richard L.

    2006-01-01

    Recent evidence suggests that ventricular ejection is partly powered by a delayed development of force, i.e., stretch activation, in regions of the ventricular wall due to stretch resulting from torsional twist of the ventricle around the apex-to-base axis. Given the potential importance of stretch activation in cardiac function, we characterized the stretch activation response and its Ca2+ dependence in murine skinned myocardium at 22°C in solutions of varying Ca2+ concentrations. Stretch activation was induced by suddenly imposing a stretch of 0.5–2.5% of initial length to the isometrically contracting muscle and then holding the muscle at the new length. The force response to stretch was multiphasic: force initially increased in proportion to the amount of stretch, reached a peak, and then declined to a minimum before redeveloping to a new steady level. This last phase of the response is the delayed force characteristic of myocardial stretch activation and is presumably due to increased attachment of cross-bridges as a consequence of stretch. The amplitude and rate of stretch activation varied with Ca2+ concentration and more specifically with the level of isometric force prior to the stretch. Since myocardial force is regulated both by Ca2+ binding to troponin-C and cross-bridge binding to thin filaments, we explored the role of cross-bridge binding in the stretch activation response using NEM-S1, a strong-binding, non-force–generating derivative of myosin subfragment 1. NEM-S1 treatment at submaximal Ca2+-activated isometric forces significantly accelerated the rate of the stretch activation response and reduced its amplitude. These data show that the rate and amplitude of myocardial stretch activation vary with the level of activation and that stretch activation involves cooperative binding of cross-bridges to the thin filament. Such a mechanism would contribute to increased systolic ejection in response to increased delivery of activator Ca2+ during excitation–contraction coupling. PMID:16446502

  9. Joint kinetic determinants of starting block performance in athletic sprinting.

    PubMed

    Brazil, Adam; Exell, Timothy; Wilson, Cassie; Willwacher, Steffen; Bezodis, Ian N; Irwin, Gareth

    2018-07-01

    The aim of this study was to explore the relationships between lower limb joint kinetics, external force production and starting block performance (normalised average horizontal power, NAHP). Seventeen male sprinters (100 m PB, 10.67 ± 0.32 s) performed maximal block starts from instrumented starting blocks (1000 Hz) whilst 3D kinematics (250 Hz) were also recorded during the block phase. Ankle, knee and hip resultant joint moment and power were calculated at the rear and front leg using inverse dynamics. Average horizontal force applied to the front (r = 0.46) and rear (r = 0.44) block explained 86% of the variance in NAHP. At the joint level, many "very likely" to "almost certain" relationships (r = 0.57 to 0.83) were found between joint kinetic data and the magnitude of horizontal force applied to each block although stepwise multiple regression revealed that 55% of the variance in NAHP was accounted for by rear ankle moment, front hip moment and front knee power. The current study provides novel insight into starting block performance and the relationships between lower limb joint kinetic and external kinetic data that can help inform physical and technical training practices for this skill.

  10. Maximum likelihood estimation of protein kinetic parameters under weak assumptions from unfolding force spectroscopy experiments

    NASA Astrophysics Data System (ADS)

    Aioanei, Daniel; Samorì, Bruno; Brucale, Marco

    2009-12-01

    Single molecule force spectroscopy (SMFS) is extensively used to characterize the mechanical unfolding behavior of individual protein domains under applied force by pulling chimeric polyproteins consisting of identical tandem repeats. Constant velocity unfolding SMFS data can be employed to reconstruct the protein unfolding energy landscape and kinetics. The methods applied so far require the specification of a single stretching force increase function, either theoretically derived or experimentally inferred, which must then be assumed to accurately describe the entirety of the experimental data. The very existence of a suitable optimal force model, even in the context of a single experimental data set, is still questioned. Herein, we propose a maximum likelihood (ML) framework for the estimation of protein kinetic parameters which can accommodate all the established theoretical force increase models. Our framework does not presuppose the existence of a single force characteristic function. Rather, it can be used with a heterogeneous set of functions, each describing the protein behavior in the stretching time range leading to one rupture event. We propose a simple way of constructing such a set of functions via piecewise linear approximation of the SMFS force vs time data and we prove the suitability of the approach both with synthetic data and experimentally. Additionally, when the spontaneous unfolding rate is the only unknown parameter, we find a correction factor that eliminates the bias of the ML estimator while also reducing its variance. Finally, we investigate which of several time-constrained experiment designs leads to better estimators.

  11. Integrated planning and spatial evaluation of megasite remediation and reuse options.

    PubMed

    Schädler, Sebastian; Morio, Maximilian; Bartke, Stephan; Finkel, Michael

    2012-01-01

    Redevelopment of large contaminated brownfields (megasites) is often hampered by a lack of communication and harmonization among diverse stakeholders with potentially conflicting interests. Decision support is required to provide integrative yet transparent evaluation of often complex spatial information to stakeholders with different areas of expertise. It is considered crucial for successful redevelopment to identify a shared vision of how the respective contaminated site could be remediated and redeveloped. We describe a framework of assessment methods and models that analyzes and visualizes site- and land use-specific spatial information at the screening level, with the aim to support the derivation of recommendable land use layouts and to initiate further and more detailed planning. The framework integrates a GIS-based identification of areas to be remediated, an estimation of associated clean-up costs, a spatially explicit market value appraisal, and an assessment of the planned future land use's contribution to sustainable urban and regional development. Case study results show that derived options are potentially favorable in both a sustainability and an economic sense and that iterative re-planning is facilitated by the evaluation and visualization of economic, ecological and socio-economic aspects. The framework supports an efficient early judgment about whether and how abandoned land may be assigned a sustainable and marketable land use. Copyright © 2011 Elsevier B.V. All rights reserved.

  12. 32 CFR 174.13 - Personal property.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... for redevelopment planning purposes. (e) Personal property may be removed upon approval of the... property together with the real property, will be transferred to the Defense Reutilization and Marketing...

  13. A two-fluid study of oblique tearing modes in a force-free current sheet

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Akçay, Cihan, E-mail: akcay@lanl.gov; Daughton, William; Lukin, Vyacheslav S.

    2016-01-15

    Kinetic simulations have demonstrated that three-dimensional reconnection in collisionless regimes proceeds through the formation and interaction of magnetic flux ropes, which are generated due to the growth of tearing instabilities at multiple resonance surfaces. Since kinetic simulations are intrinsically expensive, it is desirable to explore the feasibility of reduced two-fluid models to capture this complex evolution, particularly, in the strong guide field regime, where two-fluid models are better justified. With this goal in mind, this paper compares the evolution of the collisionless tearing instability in a force-free current sheet with a two-fluid model and fully kinetic simulations. Our results indicatemore » that the most unstable modes are oblique for guide fields larger than the reconnecting field, in agreement with the kinetic results. The standard two-fluid tearing theory is extended to address the tearing instability at oblique angles. The resulting theory yields a flat oblique spectrum and underestimates the growth of oblique modes in a similar manner to kinetic theory relative to kinetic simulations.« less

  14. A two-fluid study of oblique tearing modes in a force-free current sheet

    DOE PAGES

    Akçay, Cihan; Daughton, William; Lukin, Vyacheslav S.; ...

    2016-01-01

    Kinetic simulations have demonstrated that three-dimensional reconnection in collisionless regimes proceeds through the formation and interaction of magnetic flux ropes, which are generated due to the growth of tearing instabilities at multiple resonance surfaces. Because kinetic simulations are intrinsically expensive, it is desirable to explore the feasibility of reduced two-fluid models to capture this complex evolution, particularly, in the strong guide field regime, where two-fluid models are better justified. With this goal in mind, this paper compares the evolution of the collisionless tearing instability in a force-free current sheet with a two-fluid model and fully kinetic simulations. Our results indicatemore » that the most unstable modes are oblique for guide fields larger than the reconnecting field, in agreement with the kinetic results. The standard two-fluid tearing theory is extended to address the tearing instability at oblique angles. As a results this theory yields a flat oblique spectrum and underestimates the growth of oblique modes in a similar manner to kinetic theory relative to kinetic simulations.« less

  15. Multijoint kinetic chain analysis of knee extension during the soccer instep kick.

    PubMed

    Naito, Kozo; Fukui, Yosuke; Maruyama, Takeo

    2010-04-01

    Although previous studies have shown that motion-dependent interactions between adjacent segments play an important role in producing knee extension during the soccer instep kick, detailed knowledge about the mechanisms underlying those interactions is lacking. The present study aimed to develop a 3-D dynamical model for the multijoint kinetic chain of the instep kick in order to quantify the contributions of the causal dynamical factors to the production of maximum angular velocity during knee extension. Nine collegiate soccer players volunteered to participate in the experiment and performed instep kicking movements while 3-D positional data and the ground reaction force were measured. A dynamical model was developed in the form of a linked system containing 8 segments and 18 joint rotations, and the knee extension/flexion motion was decomposed into causal factors related to muscular moment, gyroscopic moment, centrifugal force, Coriolis force, gravity, proximal endpoint linear acceleration, and external force-dependent terms. The rapid knee extension during instep kicking was found to result almost entirely from kicking leg centrifugal force, trunk rotation muscular moment, kicking leg Coriolis force, and trunk rotation gyroscopic-dependent components. Based on the finding that rapid knee extension during instep kicking stems from multiple dynamical factors, it is suggested that the multijoint kinetic chain analysis used in the present study is more useful for achieving a detailed understanding of the cause of rapid kicking leg movement than the previously used 2-D, two-segment kinetic chain model. The present results also indicated that the centrifugal effect due to the kicking hip flexion angular velocity contributed substantially to the generation of a rapid knee extension, suggesting that the adjustment between the kicking hip flexion angular velocity and the leg configuration (knee flexion angle) is more important for effective instep kicking than other joint kinematics.

  16. Kinetic analysis of concurrent activation potentiation during back squats and jump squats.

    PubMed

    Ebben, William P; Kaufmann, Clare E; Fauth, McKenzie L; Petushek, Erich J

    2010-06-01

    Concurrent activation potentiation enhances muscular force during open kinetic chain isometric and isokinetic exercises via remote voluntary contractions (RVCs). The purpose of this study was to evaluate the effect of RVCs on the performance of closed kinetic chain ground-based exercises. Subjects included 13 men (21.4+/-1.5 years) who performed the back squat and jump squat in 2 test conditions. The RVC condition included performing the test exercises while clenching the jaw on a mouth guard, forcefully gripping and pulling the barbell down into the trapezius, and performing a Valsalva maneuver. The normal condition (NO-RVC) included performing the test exercises without RVCs. Exercises were assessed with a force platform. Peak ground reaction force (GRF), rate of force development (RFD) during the first 100 milliseconds (RFD-100), RFD to peak GRF (RFD-P), and jump squat height (JH) were calculated from the force-time records. Data were analyzed using an analysis of variance. Results reveal that GRF and RFD-100 were higher in the RVC compared with the NO-RVC condition for both the back squat and jump squat (p

  17. The Biotin/Avidin complex adhesion force

    NASA Astrophysics Data System (ADS)

    Balsera, Manel A.; Izrailev, Sergei; Stepaniants, Sergey; Oono, Yoshitsugu; Schulten, Klaus

    1997-03-01

    The vitamin Biotin and the protein avidin form one of the strongest non-covalent bonds between biological molecules. We have performed molecular and stochastic dynamic modeling of the unbinding of this complex(Izrailev et al., Biophysical Journal, In press). These simulations provide insight into the effect of particular residues and water on the tight binding of the system. With the aid of simple phenomenological models we have related qualitatively our results to Atomic Force Microscopy adhesion force measurements (E.-L. Florin, V. T. Moy and H. E. Gaub Science) 264:415-417 and kinetic dissociation experiments( A. Chilcotti and P. S. Stayton, J. Am. Chem. Soc.) 117:10622-10628. We will discuss the difficulties preventing a more quantitative understanding of the unbinding force and kinetics.

  18. The Common Forces: Conservative or Nonconservative?

    ERIC Educational Resources Information Center

    Keeports, David

    2006-01-01

    Of the forces commonly encountered when solving problems in Newtonian mechanics, introductory texts usually limit illustrations of the definitions of conservative and nonconservative forces to gravity, spring forces, kinetic friction and fluid resistance. However, at the expense of very little class time, the question of whether each of the common…

  19. Single-molecule force measurement via optical tweezers reveals different kinetic features of two BRaf mutants responsible for cardio-facial-cutaneous (CFC) syndrome

    PubMed Central

    Wen, Cheng; Ye, Anpei

    2013-01-01

    BRaf (B- Rapid Accelerated Fibrosarcoma) protein is an important serine/threonine-protein kinase. Two domains on BRaf can independently bind its upstream kinase, Ras (Rat Sarcoma) protein. These are the Ras binding domain (RBD) and cysteine-rich-domain (CRD). Herein we use customized optical tweezers to compare the Ras binding process in two pathological mutants of BRaf responsible for CFC syndrome, abbreviated BRaf (A246P) and BRaf (Q257R). The two mutants differ in their kinetics of Ras-binding, though both bind Ras with similar increased overall affinity. BRaf (A246P) exhibits a slightly higher Ras/CRD unbinding force and a significantly higher Ras/RBD unbinding force versus the wild type. The contrary phenomenon is observed in the Q257R mutation. Simulations of the unstressed-off rate, koff(0), yield results in accordance with the changes revealed by the mean unbinding force. Our approach can be applied to rapidly assess other mutated proteins to deduce the effects of mutation on their kinetics compared to wild type proteins and to each other. PMID:24409384

  20. Resolving DNA-ligand intercalation in the entropic stretching regime

    NASA Astrophysics Data System (ADS)

    Almaqwashi, Ali A.

    Single molecule studies of DNA intercalation are typically conducted by applying stretching forces to obtain force-dependent DNA elongation measurements. The zero-force properties of DNA intercalation are determined by equilibrium and kinetic force-analysis. However, the applied stretching forces that are above the entropic regime (>5 pN) prevent DNA-DNA contact which may eliminate competitive DNA-ligand interactions. In particular, it is noted that cationic mono-intercalators investigated by single molecule force spectroscopy are mostly found to intercalate DNA with single rate, while bulk studies reported additional slower rates. Here, a proposed framework quantifies DNA intercalation by cationic ligands in competition with relatively rapid kinetic DNA-ligand aggregation. At a constant applied force in the entropic stretching regime, the analysis illustrates that DNA intercalation would be measurably optimized only within a narrow range of low ligand concentrations. As DNA intercalators are considered for potential DNA-targeted therapeutics, this analysis provides insights in tuning ligand concertation to maximize therapeutics efficiency.

  1. Brownfields Green Avenue Sites: Technical Memorandum - Conceptual Design for Sustainable Redevelopment

    EPA Pesticide Factsheets

    This technical memorandum briefly describes the site and proposed conceptual site plan, indicates conceptual design considerations, specifies recommended green and sustainable features, and offers other recommendations

  2. 26 CFR 1.103A-2 - Qualified mortgage bond.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... monthly gross pay, any additional income from investments, pensions, Veterans Administration (VA...; (iv) whether proceeds targeted to each area are to be used to finance redevelopment of existing...

  3. 26 CFR 1.103A-2 - Qualified mortgage bond.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... monthly gross pay, any additional income from investments, pensions, Veterans Administration (VA...; (iv) whether proceeds targeted to each area are to be used to finance redevelopment of existing...

  4. 26 CFR 1.103A-2 - Qualified mortgage bond.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... monthly gross pay, any additional income from investments, pensions, Veterans Administration (VA...; (iv) whether proceeds targeted to each area are to be used to finance redevelopment of existing...

  5. The influence of spatially and temporally high-resolution wind forcing on the power input to near-inertial waves in the ocean

    NASA Astrophysics Data System (ADS)

    Rimac, A.; Eden, C.; von Storch, J.

    2012-12-01

    Coexistence of stable stratification, the meridional overturning circulation and meso-scale eddies and their influence on the ocean's circulation still raise complex questions concerning the ocean energetics. Oceanic general circulation is mainly forced by the wind field and deep water tides. Its essential energetics are the conversion of kinetic energy of the winds and tides into oceanic potential and kinetic energy. Energy needed for the circulation is bound to internal wave fields. Direct internal wave generation by the wind at the sea surface is one of the sources of this energy. Previous studies using mixed-layer type of models and low frequency wind forcings (six-hourly and daily) left room for improvement. Using mixed-layer models it is not possible to assess the distribution of near-inertial energy into the deep ocean. Also, coarse temporal resolution of wind forcing strongly underestimates the near-inertial wave energy. To overcome this difficulty we use a high resolution ocean model with high frequency wind forcings. We establish the following model setup: We use the Max Planck Institute Ocean Model (MPIOM) on a tripolar grid with 45km horizontal resolution and 40 vertical levels. We run the model with wind forcings that vary in horizontal (250km versus 40km) and temporal resolution (six versus one-hourly). In our study we answer the following questions: How big is the wind kinetic energy input to the near-inertial waves? Is the kinetic energy of the near-inertial waves enhanced when high-frequency wind forcings are used? If so, by how much and why, due to higher level of temporal wind variability or due to better spatial representation of the near-inertial waves? How big is the total power of near-inertial waves generated by the wind at the surface of the ocean? We run the model for one year. Our model results show that the near-inertial waves are excited both using wind forcings of high and low horizontal and temporal resolution. Near-inertial energy is almost two times higher when we force the model with high frequency wind forcings. The influence on the energy mostly depends on the time difference between two forcing fields while the spatial difference has little influence.

  6. Evaluation of frictional resistance and surface characteristics after immersion of orthodontic brackets and wire in different chemical solutions: A comparative in vitrostudy.

    PubMed

    Nanjundan, Kavitha; Vimala, G

    2016-01-01

    To evaluate the changes of static and kinetic frictional forces between the brackets and wires following exposure to a soft drink, acidic food ingredient, and acidulated fluoride prophylactic agents. Two types of Roth prescription mandibular incisor brackets were used: 3M Unitek Victory stainless steel (SS) brackets (n = 40) and Transcend 6000 polycrystalline alumina (PCA) brackets (n = 40) as well as eighty 0.019 × 0.025" dimension ortho technology SS wires of 50 mm length each. Subsequently, brackets tied with SS wires divided into eight subgroups (n = 10) and were immersed in vinegar (pH = 3.5 ± 0.5), Pepsi ® (pH = 2.46), Colgate Phos-Flur mouth rinse (pH = 5.1), and artificial saliva (control group pH = 7) for 24 h. Changes in surface morphology under scanning electron microscope ×1000, surface roughness (Ra) with surface profilometer (single bracket and single wire from each subgroup), and frictional resistance using universal testing machine were evaluated. Highest mean (standard deviation) static frictional force of 2.65 (0.25) N was recorded in Pepsi ® followed by 2.57 (0.25) N, 2.40 (0.22) N, and 2.36 (0.17) N for Vinegar, Colgate Phos-Flur mouth rinse, and artificial saliva groups, respectively. In a similar order, lesser mean kinetic frictional forces obtained. PCA brackets revealed more surface deterioration and higher frictional force values than SS brackets. A significant positive correlation was observed between frictional forces and bracket slot roughness (r = 0.861 and 0.802, respectively, for static and kinetic frictional forces, p < 0.001 for both) and wire roughness (r = 0.243 and 0.242, respectively, for static and kinetic frictional forces, p < 0.05 for both). Findings may have long-term implications when acidic food substances are used during fixed orthodontic treatment. Further, in vivo studies are required to analyze the clinical effect of acidic mediums in the oral environment during orthodontic treatment.

  7. Analysis of professors' perceptions towards institutional redevelopment of brownfield sites in Alabama

    NASA Astrophysics Data System (ADS)

    King, Berkley Nathaniel, Jr.

    This study was conducted to analyze professors' perceptions on the institutional redevelopment of brownfield sites into usable greenspaces. The U.S. Environmental Protection Agency (2016) refers to brownfields as sites, (either facility or land) under public law § 107-118 (H.R. 2869), which are contaminated with a substance that is classified as a hazard or a pollutant. Usable greenspaces, however, are open spaces or any open piece of land that is undeveloped, has no buildings or other built structures, and is accessible to the public (EPA, 2015). Open green spaces provide recreational areas for residents and help to enhance the beauty and environmental quality of neighborhoods (EPA, 2015). In addition, in a study conducted by Dadvand et al. (2015), exposure to green space has been associated with better physical and mental health among elementary school children, and this exposure, according to Dadvand et al., could also influence cognitive development. Because of the institutional context provided in these articles and other research studies, a sequential mixed-methods study was conducted that investigated the perceptions of professors towards the redevelopment of brownfields near their campuses. This study provided demographics of forty-two college and university professors employed at two institutions in the state of Alabama, a southeastern region of the United States. Survey questions were structured to analyze qualitative data. The secondary method of analysis utilized descriptive statistics to measure the most important indicators that influences professors' perceptions. The collection of quantitative data was adapted from an instrument designed by Wernstedt, Crooks, & Hersh (2003). Findings from the study showed that professors are knowledgeable and aware of the sociological and economic challenges in low income communities where brownfields are geographically located. Pseudonyms are used for the three universities which were contacted. Findings also indicate that Eta-One University is a recipient of an EPA Region 4 grant that focuses on educating low income communities in areas where brownfield sites are located. Recommendations from the study will be provided to local, state, and federal government agencies resulting from this data on professors' perceptions on the redevelopment of brownfield sites and the role in which universities and college professors play.

  8. Kinetic Determinants of Reactive Strength in Highly Trained Sprint Athletes.

    PubMed

    Douglas, Jamie; Pearson, Simon; Ross, Angus; McGuigan, Mike

    2018-06-01

    Douglas, J, Pearson, S, Ross, A, and McGuigan, M. Kinetic determinants of reactive strength in highly trained sprint athletes. J Strength Cond Res 32(6): 1562-1570, 2018-The purpose of this study was to determine the braking and propulsive phase kinetic variables underpinning reactive strength in highly trained sprint athletes in comparison with a nonsprint-trained control group. Twelve highly trained sprint athletes and 12 nonsprint-trained participants performed drop jumps (DJs) from 0.25, 0.50, and 0.75 m onto a force plate. One familiarization session was followed by an experimental testing session within the same week. Reactive strength index (RSI), contact time, flight time, and leg stiffness were determined. Kinetic variables including force, power, and impulse were assessed within the braking and propulsive phases. Sprint-trained athletes demonstrated higher RSI vs. nonsprint-trained participants across all drop heights {3.02 vs. 2.02; ES (±90% confidence limit [CL]): 3.11 ± 0.86}. This difference was primarily attained by briefer contact times (0.16 vs. 0.22 seconds; effect size [ES]: -1.49 ± 0.53) with smaller differences observed for flight time (0.50 vs. 0.46 seconds; ES: 0.53 ± 0.58). Leg stiffness, braking and propulsive phase force, and power were higher in sprint-trained athletes. Very large differences were observed in mean braking force (51 vs. 38 N·kg; ES: 2.57 ± 0.73) which was closely associated with contact time (r ±90% CL: -0.93 ± 0.05). Sprint-trained athletes exhibited superior reactive strength than nonsprint-trained participants. This was due to the ability to strike the ground with a stiffer leg spring, an enhanced expression of braking force, and possibly an increased utilization of elastic structures. The DJ kinetic analysis provides additional insight into the determinants of reactive strength which may inform subsequent testing and training.

  9. Force-dependent isomerization kinetics of a highly conserved proline switch modulates the mechanosensing region of filamin

    PubMed Central

    Rognoni, Lorenz; Möst, Tobias; Žoldák, Gabriel; Rief, Matthias

    2014-01-01

    Proline switches, controlled by cis–trans isomerization, have emerged as a particularly effective regulatory mechanism in a wide range of biological processes. In this study, we use single-molecule mechanical measurements to develop a full kinetic and energetic description of a highly conserved proline switch in the force-sensing domain 20 of human filamin and how prolyl isomerization modulates the force-sensing mechanism. Proline isomerization toggles domain 20 between two conformations. A stable cis conformation with slow unfolding, favoring the autoinhibited closed conformation of filamin’s force-sensing domain pair 20–21, and a less stable, uninhibited conformation promoted by the trans form. The data provide detailed insight into the folding mechanisms that underpin the functionality of this binary switch and elucidate its remarkable efficiency in modulating force-sensing, thus combining two previously unconnected regulatory mechanisms, proline switches and mechanosensing. PMID:24706888

  10. Kinetics of the head-neck complex in low-speed rear impact.

    PubMed

    Stemper, Brian D; Yoganandan, Naryan; Pintar, Frank A

    2003-01-01

    A comprehensive characterization of the biomechanics of the cervical spine in rear impact will lead to an understanding of the mechanisms of whiplash injury. Cervical kinematics have been experimentally described using human volunteers, full-body cadaver specimens, and isolated and intact head-neck specimens. However, forces and moments at the cervico-thoracic junction have not been clearly delineated. An experimental investigation was performed using ten intact head-neck complexes to delineate the loading at the base of the cervical spine and angular acceleration of the head in whiplash. A pendulum-minisled apparatus was used to simulate whiplash acceleration of the thorax at four impact severities. Lower neck loads were measured using a six-axis load cell attached between the minisled and head-neck specimens, and head angular motion was measured with an angular rate sensor attached to the lateral side of the head. Shear and axial force, extension moment, and head angular acceleration increased with impact severity. Shear force was significantly larger than axial force (p < 0.0001). Shear force reached its maximum value at 46 msec. Maximum extension moment occurred between 7 and 22 msec after maximum shear force. Maximum angular acceleration of the head occurred 2 to 18 msec later. Maximum axial force occurred last (106 msec). All four kinetic components reached maximum values during cervical S-curvature, with maximum shear force and extension moment occurring before the attainment of maximum S-curvature. Results of the present investigation indicate that shear force and extension moment at the cervico-thoracic junction drive the non-physiologic cervical S-curvature responsible for whiplash injury and underscore the importance of understanding cervical kinematics and the underlying kinetics.

  11. Casimir rack and pinion as a miniaturized kinetic energy harvester

    NASA Astrophysics Data System (ADS)

    Miri, MirFaez; Etesami, Zahra

    2016-08-01

    We study a nanoscale machine composed of a rack and a pinion with no contact, but intermeshed via the lateral Casimir force. We adopt a simple model for the random velocity of the rack subject to external random forces, namely, a dichotomous noise with zero mean value. We show that the pinion, even when it experiences random thermal torque, can do work against a load. The device thus converts the kinetic energy of the random motions of the rack into useful work.

  12. Symmetrical kinematics does not imply symmetrical kinetics in people with transtibial amputation using cycling model.

    PubMed

    Childers, W Lee; Kogler, Géza F

    2014-01-01

    People with amputation move asymmetrically with regard to kinematics (joint angles) and kinetics (joint forces and moments). Clinicians have traditionally sought to minimize kinematic asymmetries, assuming kinetic asymmetries would also be minimized. A cycling model evaluated locomotor asymmetries. Eight individuals with unilateral transtibial amputation pedaled with 172 mm-length crank arms on both sides (control condition) and with the crank arm length shortened to 162 mm on the amputated side (CRANK condition). Pedaling kinetics and limb kinematics were recorded. Joint kinetics, joint angles (mean and range of motion [ROM]), and pedaling asymmetries were calculated from force pedals and with a motion capture system. A one-way analysis of variance with tukey post hoc compared kinetics and kinematics across limbs. Statistical significance was set to p

  13. Folding kinetics of WW domains with the united residue force field for bridging microscopic motions and experimental measurements

    PubMed Central

    Zhou, Rui; Maisuradze, Gia G.; Suñol, David; Todorovski, Toni; Macias, Maria J.; Xiao, Yi; Scheraga, Harold A.; Czaplewski, Cezary; Liwo, Adam

    2014-01-01

    To demonstrate the utility of the coarse-grained united-residue (UNRES) force field to compare experimental and computed kinetic data for folding proteins, we have performed long-time millisecond-timescale canonical Langevin molecular dynamics simulations of the triple β-strand from the Formin binding protein 28 WW domain and six nonnatural variants, using UNRES. The results have been compared with available experimental data in both a qualitative and a quantitative manner. Complexities of the folding pathways, which cannot be determined experimentally, were revealed. The folding mechanisms obtained from the simulated folding kinetics are in agreement with experimental results, with a few discrepancies for which we have accounted. The origins of single- and double-exponential kinetics and their correlations with two- and three-state folding scenarios are shown to be related to the relative barrier heights between the various states. The rate constants obtained from time profiles of the fractions of the native, intermediate, and unfolded structures, and the kinetic equations fitted to them, correlate with the experimental values; however, they are about three orders of magnitude larger than the experimental ones for most of the systems. These differences are in agreement with the timescale extension derived by scaling down the friction of water and averaging out the fast degrees of freedom when passing from all-atom to a coarse-grained representation. Our results indicate that the UNRES force field can provide accurate predictions of folding kinetics of these WW domains, often used as models for the study of the mechanisms of proein folding. PMID:25489078

  14. Folding kinetics of WW domains with the united residue force field for bridging microscopic motions and experimental measurements.

    PubMed

    Zhou, Rui; Maisuradze, Gia G; Suñol, David; Todorovski, Toni; Macias, Maria J; Xiao, Yi; Scheraga, Harold A; Czaplewski, Cezary; Liwo, Adam

    2014-12-23

    To demonstrate the utility of the coarse-grained united-residue (UNRES) force field to compare experimental and computed kinetic data for folding proteins, we have performed long-time millisecond-timescale canonical Langevin molecular dynamics simulations of the triple β-strand from the Formin binding protein 28 WW domain and six nonnatural variants, using UNRES. The results have been compared with available experimental data in both a qualitative and a quantitative manner. Complexities of the folding pathways, which cannot be determined experimentally, were revealed. The folding mechanisms obtained from the simulated folding kinetics are in agreement with experimental results, with a few discrepancies for which we have accounted. The origins of single- and double-exponential kinetics and their correlations with two- and three-state folding scenarios are shown to be related to the relative barrier heights between the various states. The rate constants obtained from time profiles of the fractions of the native, intermediate, and unfolded structures, and the kinetic equations fitted to them, correlate with the experimental values; however, they are about three orders of magnitude larger than the experimental ones for most of the systems. These differences are in agreement with the timescale extension derived by scaling down the friction of water and averaging out the fast degrees of freedom when passing from all-atom to a coarse-grained representation. Our results indicate that the UNRES force field can provide accurate predictions of folding kinetics of these WW domains, often used as models for the study of the mechanisms of proein folding.

  15. Whole-Body Human Inverse Dynamics with Distributed Micro-Accelerometers, Gyros and Force Sensing †

    PubMed Central

    Latella, Claudia; Kuppuswamy, Naveen; Romano, Francesco; Traversaro, Silvio; Nori, Francesco

    2016-01-01

    Human motion tracking is a powerful tool used in a large range of applications that require human movement analysis. Although it is a well-established technique, its main limitation is the lack of estimation of real-time kinetics information such as forces and torques during the motion capture. In this paper, we present a novel approach for a human soft wearable force tracking for the simultaneous estimation of whole-body forces along with the motion. The early stage of our framework encompasses traditional passive marker based methods, inertial and contact force sensor modalities and harnesses a probabilistic computational technique for estimating dynamic quantities, originally proposed in the domain of humanoid robot control. We present experimental analysis on subjects performing a two degrees-of-freedom bowing task, and we estimate the motion and kinetics quantities. The results demonstrate the validity of the proposed method. We discuss the possible use of this technique in the design of a novel soft wearable force tracking device and its potential applications. PMID:27213394

  16. Calculations of Alfven Wave Driving Forces, Plasma Flow and Current Drive in Tokamak Plasmas

    NASA Astrophysics Data System (ADS)

    Elfimov, Artur; Galvao, Ricardo; Amarante-Segundo, Gesil; Nascimento, Ivan

    2000-10-01

    A general form of time-averaged poloidal ponderomotive forces induced by fast and kinetic Alfvin waves by direct numerical calculations and in geometric optics approximation are analyzed on the basis of the collisionless two fluid (ions and electrons) magneto-hydrodynamics equation. Analytical approximations are used to clarify the effect of Larmour radius on radio-frequency (RF) ponderomotive forces and on poloidal flows induced by them in tokamak plasmas.The RF ponderomotive force is expressed as a sum of a gradient part and of a wave momentum transfer force, which is proportional to wave dissipation. The gradient electromagnetic stress force is combined with fluid dynamic (Reynolds) stress force. It is shown that accounting only Reynolds stress term can overestimate the plasma flow and it is found that the finite ion Larmor radius effect play fundamental role in ponderomotive forces that can drive a poloidal flow, which is larger than a flow driven by a wave momentum transfer force. Finally, balancing the RF forces by the electron-ion friction and viscous force the current and plasma flows driven by ponderomotive forces are calculated for tokamak plasmas, using a kinetic code [Phys. Plasmas, v.6 (1999) p.2437]. Strongly sheared current and plasma flow waves is found.

  17. Conceptual Green Infrastructure Design for Washington Street, City of Sanford

    EPA Pesticide Factsheets

    Summary of the Sanford Mill Yard Complex presents an opportunity to include green infrastructure practices in a land redevelopment initiative with relative ease while providing multiple benefits to the surrounding community.

  18. Design for Resilience in Brattleboro's Lower Whetstone Brook Corridor

    EPA Pesticide Factsheets

    This report details design solutions that identify options for creating resilient redevelopment & recreational opportunities within flood prone areas of the town while protecting water quality & connecting people with the Whetstone Brook.

  19. Contractile properties of single permeabilized muscle fibers from congenital cleft palates and normal palates of Spanish goats.

    PubMed

    Hanes, Michael C; Weinzweig, Jeffrey; Kuzon, William M; Panter, Kip E; Buchman, Steven R; Faulkner, John A; Yu, Deborah; Cederna, Paul S; Larkin, Lisa M

    2007-05-01

    Analysis of the composition of muscle fibers constituent to a cleft palate could provide significant insight into the cause of velopharyngeal inadequacy. The authors hypothesized that levator veli palatini muscle dysfunction inherent to cleft palates could affect the timing and outcome of cleft palate repair. Single, permeabilized muscle fibers from levator veli palatini muscles of three normal (n = 19 fibers) and three chemically induced congenital cleft palates (n = 21 fibers) of 14-month-old goats were isolated, and contractile properties were evaluated. The maximum isometric force and rate constants of tension redevelopment (ktr) were measured, and the specific force and normalized power were calculated for each fiber. The ktr measures indicate that cleft fibers are predominantly fast-fatigable; normal fibers are slow fatigue-resistant: after a 10-minute isometric contraction, fibers from cleft palates had a loss of force 16 percent greater than that from normal palates (p = 0.0001). The cross-sectional areas of the fibers from cleft palates (2750 +/- 209 microm2) were greater (p = 0.05) than those from normal palates (2226 +/- 143 microm2). Specific forces did not differ between the two groups. Maximum normalized power of fibers from cleft palates (11.05 +/- 1.82 W/l) was greater (p = 0.0001) than fibers from normal palates (1.60 +/- 0.12 W/l). There are clear physiologic differences in single muscle fibers from cleft palates and normal palates: cleft palate fibers are physiologically fast, have greater fatigability, and have greater power production. Detection of functional and/or fiber type differences in muscles of cleft palates may provide preoperative identification of a patient's susceptibility to velopharyngeal inadequacy and permit early surgical intervention to correct this clinical condition.

  20. Harmonic force spectroscopy measures load-dependent kinetics of individual human β-cardiac myosin molecules.

    PubMed

    Sung, Jongmin; Nag, Suman; Mortensen, Kim I; Vestergaard, Christian L; Sutton, Shirley; Ruppel, Kathleen; Flyvbjerg, Henrik; Spudich, James A

    2015-08-04

    Molecular motors are responsible for numerous cellular processes from cargo transport to heart contraction. Their interactions with other cellular components are often transient and exhibit kinetics that depend on load. Here, we measure such interactions using 'harmonic force spectroscopy'. In this method, harmonic oscillation of the sample stage of a laser trap immediately, automatically and randomly applies sinusoidally varying loads to a single motor molecule interacting with a single track along which it moves. The experimental protocol and the data analysis are simple, fast and efficient. The protocol accumulates statistics fast enough to deliver single-molecule results from single-molecule experiments. We demonstrate the method's performance by measuring the force-dependent kinetics of individual human β-cardiac myosin molecules interacting with an actin filament at physiological ATP concentration. We show that a molecule's ADP release rate depends exponentially on the applied load, in qualitative agreement with cardiac muscle, which contracts with a velocity inversely proportional to external load.

  1. Harmonic force spectroscopy measures load-dependent kinetics of individual human β-cardiac myosin molecules

    PubMed Central

    Sung, Jongmin; Nag, Suman; Mortensen, Kim I.; Vestergaard, Christian L.; Sutton, Shirley; Ruppel, Kathleen; Flyvbjerg, Henrik; Spudich, James A.

    2015-01-01

    Molecular motors are responsible for numerous cellular processes from cargo transport to heart contraction. Their interactions with other cellular components are often transient and exhibit kinetics that depend on load. Here, we measure such interactions using ‘harmonic force spectroscopy'. In this method, harmonic oscillation of the sample stage of a laser trap immediately, automatically and randomly applies sinusoidally varying loads to a single motor molecule interacting with a single track along which it moves. The experimental protocol and the data analysis are simple, fast and efficient. The protocol accumulates statistics fast enough to deliver single-molecule results from single-molecule experiments. We demonstrate the method's performance by measuring the force-dependent kinetics of individual human β-cardiac myosin molecules interacting with an actin filament at physiological ATP concentration. We show that a molecule's ADP release rate depends exponentially on the applied load, in qualitative agreement with cardiac muscle, which contracts with a velocity inversely proportional to external load. PMID:26239258

  2. Kinetic model for the long term stability of contaminated monoatomic nanowires

    NASA Astrophysics Data System (ADS)

    Vélez, P.; Dassie, S. A.; Leiva, E. P. M.

    2010-03-01

    The kinetic behavior of pure Au nanowires upon rupture is analyzed in comparison with that of nanowires contaminated with H and C. The latter present larger activation energies for the rupture for all elongations and as a consequence these contaminated wires are predicted to live longer, as observed experimentally. The kinetic analysis is complemented by an analysis in terms of the concept of reaction force. It is found that this is the relevant quantity to analyze when considering the stability of the nanowires in the limit of low elongations rates or relatively high temperatures rather than the longitudinal force acting on the nanowire. The elastic and electronic contributions to the rupture process are obtained for each system. While the latter are similar in all cases, the elastic part is found to play the decisive role to make the difference. The present results suggest that measurement of the force constant of the junction may help to determine the chemical nature of the impurity.

  3. Second order kinetic theory of parallel momentum transport in collisionless drift wave turbulence

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Li, Yang, E-mail: lyang13@mails.tsinghua.edu.cn; Southwestern Institute of Physics, Chengdu 610041; Gao, Zhe

    A second order kinetic model for turbulent ion parallel momentum transport is presented. A new nonresonant second order parallel momentum flux term is calculated. The resonant component of the ion parallel electrostatic force is the momentum source, while the nonresonant component of the ion parallel electrostatic force compensates for that of the nonresonant second order parallel momentum flux. The resonant component of the kinetic momentum flux can be divided into three parts, including the pinch term, the diffusive term, and the residual stress. By reassembling the pinch term and the residual stress, the residual stress can be considered as amore » pinch term of parallel wave-particle resonant velocity, and, therefore, may be called as “resonant velocity pinch” term. Considering the resonant component of the ion parallel electrostatic force is the transfer rate between resonant ions and waves (or, equivalently, nonresonant ions), a conservation equation of the parallel momentum of resonant ions and waves is obtained.« less

  4. A Parametric Approach to Numerical Modeling of TKR Contact Forces

    PubMed Central

    Lundberg, Hannah J.; Foucher, Kharma C.; Wimmer, Markus A.

    2009-01-01

    In vivo knee contact forces are difficult to determine using numerical methods because there are more unknown forces than equilibrium equations available. We developed parametric methods for computing contact forces across the knee joint during the stance phase of level walking. Three-dimensional contact forces were calculated at two points of contact between the tibia and the femur, one on the lateral aspect of the tibial plateau, and one on the medial side. Muscle activations were parametrically varied over their physiologic range resulting in a solution space of contact forces. The obtained solution space was reasonably small and the resulting force pattern compared well to a previous model from the literature for kinematics and external kinetics from the same patient. Peak forces of the parametric model and the previous model were similar for the first half of the stance phase, but differed for the second half. The previous model did not take into account the transverse external moment about the knee and could not calculate muscle activation levels. Ultimately, the parametric model will result in more accurate contact force inputs for total knee simulators, as current inputs are not generally based on kinematics and kinetics inputs from TKR patients. PMID:19155015

  5. Following aptamer-ricin specific binding by single molecule recognition and force spectroscopy measurements

    USDA-ARS?s Scientific Manuscript database

    The atomic force microscope (AFM) recognition and dynamic force spectroscopy (DFS) experiments provide both morphology and interaction information of the aptamer and protein, which can be used for the future study on the thermodynamics and kinetics properties of ricin-aptamer/antibody interactions. ...

  6. A model stomach system to investigate disintegration kinetics of solid foods during gastric digestion.

    PubMed

    Kong, F; Singh, R P

    2008-06-01

    Knowledge of the disintegration kinetics of food particulates in the human stomach is essential for assessing the bioaccessibility of nutrients in solid foods and understanding stomach emptying. The objective of this study was to develop a model stomach system and to investigate the kinetics of food disintegration. Our system consisted mainly of a turntable and a jacketed glass chamber containing simulated gastric juice in which plastic beads were added to simulate food particulates as well as provide a suitable mechanical destructive force on food samples. The mechanical force on the samples was simultaneously measured using the load cell of a TA-XT2 texture analyzer. Cylindrical carrots and ham samples were used as representative foods. The system is capable of simulating the in vivo stomach in terms of providing a wide range of continuous and periodic forces comparable to those measured in vivo. The modified power exponential function of the form y(t)= 1 - (1 -e(-kt))(beta), where y(t) is the mass retention ratio at time t, provided a reasonable description for the disintegration performance of tested foods. The mass retention curve can be either a sigmoidal decay with an initial delay or an exponential decay, which are decided largely by the hardness of the foods during digestion and the extent of physical force acting on the foods. A good match was observed between the kinetics of food disintegration and in vivo stomach emptying.

  7. Effect of knee and trunk angle on kinetic variables during the isometric midthigh pull: test-retest reliability.

    PubMed

    Comfort, Paul; Jones, Paul A; McMahon, John J; Newton, Robert

    2015-01-01

    The isometric midthigh pull (IMTP) has been used to monitor changes in force, maximum rate of force development (mRFD), and impulse, with performance in this task being associated with performance in athletic tasks. Numerous postures have been adopted in the literature, which may affect the kinetic variables during the task; therefore, the aim of this investigation was to determine whether different knee-joint angles (120°, 130°, 140°, and 150°) and hip-joint angles (125° and 145°), including the subjects preferred posture, affect force, mRFD, and impulse during the IMTP. Intraclass correlation coefficients demonstrated high within-session reliability (r ≥ .870, P < .001) for all kinetic variables determined in all postures, excluding impulse measures during the 130° knee-flexion, 125° hip-flexion posture, which showed a low to moderate reliability (r = .666-.739, P < .001), while between-sessions testing demonstrated high reliability (r > .819, P < .001) for all kinetic variables. There were no significant differences in peak force (P > .05, Cohen d = 0.037, power = .408), mRFD (P > .05, Cohen d = 0.037, power = .409), or impulse at 100 ms (P > .05, Cohen d = 0.056, power = .609), 200 ms (P > .05, Cohen d = 0.057, power = .624), or 300 ms (P > .05, Cohen d = 0.061, power = .656) across postures. Smallest detectable differences demonstrated that changes in performance of >1.3% in peak isometric force, >10.3% in mRFD, >5.3% in impulse at 100 ms, >4.4% in impulse at 200 ms, and >7.1% in impulse at 300 ms should be considered meaningful, irrespective of posture.

  8. Superfund Redevelopment Initiative

    EPA Pesticide Factsheets

    Provides information at the national level to ensure that at every Superfund site, EPA and its partners have an effective process and the necessary tools and information needed to return the country’s most hazardous sites to productive use.

  9. Planning Archives | NOAA Gulf Spill Restoration

    Science.gov Websites

    Artificial Reef Creation Off the Texas Coast (pdf, 1.2 MB) Galveston Island State Park Beach Redevelopment Marsh Creation (pdf, 620 KB) Louisiana: Oyster Cultch (pdf, 522 KB) Mississippi: Artificial Reef Habitat

  10. Financing Renewable Energy Projects on Contaminated Lands, Landfills, and Mine Sites

    EPA Pesticide Factsheets

    Provides information concerning financing tools and structures, as well as federal financial incentives that may be available for redeveloping potentially contaminated sites, landfills, or mine sites for renewable energy for site owners.

  11. EPA guidance : improving air quality through land use activity

    DOT National Transportation Integrated Search

    2001-01-01

    Recently states and local communities have passed hundreds of ballot initiatives preserving open space, increasing development around transit, and providing for increased brownfield redevelopment. Each of these places has had different reasons--econo...

  12. Renewable Energy Reuse and Protectiveness

    EPA Pesticide Factsheets

    EPA works collaboratively with states, tribes, local government, and other stakeholders to achieve its mission of assessing, cleaning up and restoring contaminated sites to set the stage for redevelopment or facilitate the continued use of the facility.

  13. ORD'S BROWNFIELDS RESEARCH

    EPA Science Inventory

    SMARTe is a web-based,decision support tool for developing and evaluating plans for redevelopment/revitalization of potentially contaminated sites. Brownfield project stakeholders should use SMARTe to: (1) Assess both market and non-market costs and benefits of potential redevelo...

  14. Reversing urban sprawl : a reclaimability index approach for reviving downtown brownfields.

    DOT National Transportation Integrated Search

    2011-03-01

    A key step to promoting urban revitalization is the reclamation of abandoned or underutilized contaminated sites, also known as brownfields. : Effective brownfield redevelopment approaches require environmental, socio-economic and urban planning dime...

  15. 75 FR 39581 - Yosemite Valley Plan; Yosemite National Park; Mariposa, Madera, and Tuolumne Counties, California...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-09

    ... processes in Yosemite Valley, to ameliorate environmental impacts, to preserve cultural resource values, and... of No Significant Impact (FONSI decisions) for Yosemite Lodge area redevelopment and improvements at...

  16. Solar and Wind Site Screening Decision Trees

    EPA Pesticide Factsheets

    EPA and NREL created a decision tree to guide state and local governments and other stakeholders through a process for screening sites for their suitability for future redevelopment with solar photovoltaic (PV) energy and wind energy.

  17. Estimating Monetized Benefits of Groundwater Recharge from Stormwater Retention Practices

    EPA Pesticide Factsheets

    The purpose of the study is to inform valuation of groundwater recharge from stormwater retention in areas projected for new development and redevelopment. This study examined a simplified methodology for estimating recharge volume.

  18. SOCIAL ASPECTS OF BROWNFIELD REDEVELOPMENT WORKSHOP CD

    EPA Science Inventory

    The objective of the workshop was to identify constraints, appropriate strategies and instruments to integrate social issues in urban brownfield revitalization projects oriented toward urban renewal, citizen participation and empowerment. Theoretical approaches to planning and ci...

  19. Department of Energy Forrestal Complex Redevelopment Act of 2014

    THOMAS, 113th Congress

    Rep. Norton, Eleanor Holmes [D-DC-At Large

    2014-07-28

    House - 07/29/2014 Referred to the Subcommittee on Economic Development, Public Buildings and Emergency Management. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  20. Northside Neighborhood Green Infrastructure Master Plan for Spartanburg SC

    EPA Pesticide Factsheets

    This report recommends Stormwater Management Toolbox for nonstructural and structural practices that apply to the block densities and streetscape styles proposed for the Northside redevelopment area and a designs for two land use typologies.

  1. Brownfields Langdale Mill Project: Technical Memo on Creating Inventory of Materials that can be Reused or Recycled

    EPA Pesticide Factsheets

    This technical memorandum presents the sustainability pilot activities, a summary of inventory results, and other observations and recommendations for sustainable redevelopment identified during the pilot activities.

  2. Make Development Decisions Predictable and Fair: Green Tape Program, Silver Spring, Maryland

    EPA Pesticide Factsheets

    Montgomery County's Green Tape program is making redevelopment in Silver Spring, Maryland, faster and more cost effective by speeding the permitting process for development in the mixed-use city center.

  3. Kinetic and Kinematic Analysis for Assessing the Differences in Countermovement Jump Performance in Rugby Players.

    PubMed

    Floría, Pablo; Gómez-Landero, Luis A; Suárez-Arrones, Luis; Harrison, Andrew J

    2016-09-01

    Floría, P, Gómez-Landero, LA, Suárez-Arrones, L, and Harrison, AJ. Kinetic and kinematic analysis for assessing the differences in countermovement jump performance in rugby players. J Strength Cond Res 30(9): 2533-2539, 2016-The aim of this study was to ascertain the differences in kinetic and kinematic profiles between better and poorer performers of the vertical jump within a homogeneous group of trained adults. Fifty rugby players were divided into low scoring (LOW) and high scoring (HIGH) groups based on their performance in the vertical jump. The force, velocity, displacement, and rate of force development (RFD)-time curves were analyzed to determine the differences between groups. The analysis of the data showed differences in all the patterns of the ensemble mean curves of the HIGH and LOW groups. During the eccentric phase, the differences in the HIGH group with respect to the LOW group were lower crouch position, higher downward velocity, and higher force and RFD during the braking of the downward movement. During the concentric phase, the HIGH group achieved higher upward velocity, higher force at the end of phase, and a higher position at takeoff. The higher jump performances seem to be related to a more effective stretch-shortening cycle function that is characterized by a deeper and faster countermovement with higher eccentric forces being applied to decelerate the downward movement leading to enhanced force generation during the concentric phase.

  4. The kinetics of rugby union scrummaging.

    PubMed

    Milburn, P D

    1990-01-01

    Two rugby union forward packs of differing ability levels were examined during scrummaging against an instrumented scrum machine. By systematically moving the front-row of the scrum along the scrum machine, kinetic data on each front-row forward could be obtained under all test conditions. Each forward pack was tested under the following scrummaging combinations: front-row only; front-row plus second-row; full scrum minus side-row, and full scrum. Data obtained from each scrum included the three orthogonal components of force at engagement and the sustained force applied by each front-row player. An estimate of sub-unit contributions was made by subtracting the total forward force on all three front-row players from the total for the complete scrum. Results indicated the primary role of the second-row appeared to be application of forward force. The back-row ('number eight') forward did not substantially contribute any additional forward force, and added only slightly to the lateral and vertical shear force experienced by the front-row. The side-row contributed an additional 20-27% to the forward force, but at the expense of increased vertical forces on all front-row forwards. Results of this investigation are discussed in relation to rule modification, rule interpretation and coaching.

  5. Reliability and Validity of Kinetic and Kinematic Parameters Determined With Force Plates Embedded Under a Soil-Filled Baseball Mound.

    PubMed

    Yanai, Toshimasa; Matsuo, Akifumi; Maeda, Akira; Nakamoto, Hiroki; Mizutani, Mirai; Kanehisa, Hiroaki; Fukunaga, Tetsuo

    2017-08-01

    We developed a force measurement system in a soil-filled mound for measuring ground reaction forces (GRFs) acting on baseball pitchers and examined the reliability and validity of kinetic and kinematic parameters determined from the GRFs. Three soil-filled trays of dimensions that satisfied the official baseball rules were fixed onto 3 force platforms. Eight collegiate pitchers wearing baseball shoes with metal cleats were asked to throw 5 fastballs with maximum effort from the mound toward a catcher. The reliability of each parameter was determined for each subject as the coefficient of variation across the 5 pitches. The validity of the measurements was tested by comparing the outcomes either with the true values or the corresponding values computed from a motion capture system. The coefficients of variation in the repeated measurements of the peak forces ranged from 0.00 to 0.17, and were smaller for the pivot foot than the stride foot. The mean absolute errors in the impulses determined over the entire duration of pitching motion were 5.3 N˙s, 1.9 N˙s, and 8.2 N˙s for the X-, Y-, and Z-directions, respectively. These results suggest that the present method is reliable and valid for determining selected kinetic and kinematic parameters for analyzing pitching performance.

  6. Strong DNA deformation required for extremely slow DNA threading intercalation by a binuclear ruthenium complex

    PubMed Central

    Almaqwashi, Ali A.; Paramanathan, Thayaparan; Lincoln, Per; Rouzina, Ioulia; Westerlund, Fredrik; Williams, Mark C.

    2014-01-01

    DNA intercalation by threading is expected to yield high affinity and slow dissociation, properties desirable for DNA-targeted therapeutics. To measure these properties, we utilize single molecule DNA stretching to quantify both the binding affinity and the force-dependent threading intercalation kinetics of the binuclear ruthenium complex Δ,Δ-[μ‐bidppz‐(phen)4Ru2]4+ (Δ,Δ-P). We measure the DNA elongation at a range of constant stretching forces using optical tweezers, allowing direct characterization of the intercalation kinetics as well as the amount intercalated at equilibrium. Higher forces exponentially facilitate the intercalative binding, leading to a profound decrease in the binding site size that results in one ligand intercalated at almost every DNA base stack. The zero force Δ,Δ-P intercalation Kd is 44 nM, 25-fold stronger than the analogous mono-nuclear ligand (Δ-P). The force-dependent kinetics analysis reveals a mechanism that requires DNA elongation of 0.33 nm for association, relaxation to an equilibrium elongation of 0.19 nm, and an additional elongation of 0.14 nm from the equilibrium state for dissociation. In cells, a molecule with binding properties similar to Δ,Δ-P may rapidly bind DNA destabilized by enzymes during replication or transcription, but upon enzyme dissociation it is predicted to remain intercalated for several hours, thereby interfering with essential biological processes. PMID:25245944

  7. Biomechanics of youth windmill softball pitching.

    PubMed

    Werner, Sherry L; Guido, John A; McNeice, Ryan P; Richardson, Jasper L; Delude, Neil A; Stewart, Gregory W

    2005-04-01

    Limited research attention has been paid to the potentially harmful windmill softball pitch. No information is available regarding lower extremity kinetics in softball pitching. The stresses on the throwing arm of youth windmill pitchers are clinically significant and similar to those found for college softball pitchers. Descriptive laboratory study. Three-dimensional, high-speed (240-Hz) video and stride foot force plate (1200 Hz) data were collected on fastballs from 53 youth softball pitchers. Kinematic parameters related to pitching mechanics and resultant kinetics on the throwing-arm elbow and shoulder joints were calculated. Kinetic parameters were compared to those reported for baseball pitchers. Elbow and shoulder joint loads were similar to those found for baseball pitchers and college softball pitchers. Shoulder distraction stress averaged 94% body weight for the youth pitchers. Stride foot ground reaction force patterns were not similar to those reported for baseball pitchers. Vertical and braking force components under the stride foot were in excess of body weight. Excessive distraction stress and joint torques at the throwing-arm elbow and shoulder are similar to those found in baseball pitchers, which suggests that windmill softball pitchers are at risk for overuse injuries. Normative information regarding upper and lower extremity kinematics and kinetics for 12- to 19-year-old softball pitchers has been established.

  8. A kinetic comparison of back-loading and head-loading in Xhosa women.

    PubMed

    Lloyd, R; Parr, B; Davies, S; Cooke, C

    2011-04-01

    The purpose of this study was to compare the kinetic responses associated with ground reaction force measurements to both head-loading and back-loading in a group of Xhosa women. Altogether, 16 women were divided into two groups based on their experience of head-loading. They walked over a force plate in three conditions: unloaded or carrying 20 kg in either a backpack or on their head. The most striking finding was that there was no difference in kinetic response to head-loading as a consequence of previous experience. Considering the differences between the load carriage methods, most changes were consistent with increasing load. Head-loading was, however, associated with a shorter contact time, smaller thrust maximum and greater vertical force minimum than back-loading. Both loading conditions differed from unloaded walking for a number of temporal variables associated with the ground contact phase, e.g. vertical impact peak was delayed whilst vertical thrust maximum occurred earlier. STATEMENT OF RELEVANCE: Consideration of the kinetics of head and back load carriage in African women is important from a health and safety perspective, providing an understanding of the mechanical adaptations associated with both forms of load carriage for a group of people for whom such load carriage is a daily necessity.

  9. Adsorption Mechanism of Inhibitor and Guest Molecules on the Surface of Gas Hydrates.

    PubMed

    Yagasaki, Takuma; Matsumoto, Masakazu; Tanaka, Hideki

    2015-09-23

    The adsorption of guest and kinetic inhibitor molecules on the surface of methane hydrate is investigated by using molecular dynamics simulations. We calculate the free energy profile for transferring a solute molecule from bulk water to the hydrate surface for various molecules. Spherical solutes with a diameter of ∼0.5 nm are significantly stabilized at the hydrate surface, whereas smaller and larger solutes exhibit lower adsorption affinity than the solutes of intermediate size. The range of the attractive force is subnanoscale, implying that this force has no effect on the macroscopic mass transfer of guest molecules in crystal growth processes of gas hydrates. We also examine the adsorption mechanism of a kinetic hydrate inhibitor. It is found that a monomer of the kinetic hydrate inhibitor is strongly adsorbed on the hydrate surface. However, the hydrogen bonding between the amide group of the inhibitor and water molecules on the hydrate surface, which was believed to be the driving force for the adsorption, makes no contribution to the adsorption affinity. The preferential adsorption of both the kinetic inhibitor and the spherical molecules to the surface is mainly due to the entropic stabilization arising from the presence of cavities at the hydrate surface. The dependence of surface affinity on the size of adsorbed molecules is also explained by this mechanism.

  10. The influence of spatially and temporally high-resolution wind forcing on the power input to near-inertial waves in the ocean

    NASA Astrophysics Data System (ADS)

    Rimac, Antonija; von Storch, Jin-Song; Eden, Carsten

    2013-04-01

    The estimated power required to sustain global general circulation in the ocean is about 2 TW. This power is supplied with wind stress and tides. Energy spectrum shows pronounced maxima at near-inertial frequency. Near-inertial waves excited by high-frequency winds represent an important source for deep ocean mixing since they can propagate into the deep ocean and dissipate far away from the generation sites. The energy input by winds to near-inertial waves has been studied mostly using slab ocean models and wind stress forcing with coarse temporal resolution (e.g. 6-hourly). Slab ocean models lack the ability to reproduce fundamental aspects of kinetic energy balance and systematically overestimate the wind work. Also, slab ocean models do not account the energy used for the mixed layer deepening or the energy radiating downward into the deep ocean. Coarse temporal resolution of the wind forcing strongly underestimates the near-inertial energy. To overcome this difficulty we use an eddy permitting ocean model with high-frequency wind forcing. We establish the following model setup: We use the Max Planck Institute Ocean Model (MPIOM) on a tripolar grid with 45 km horizontal resolution and 40 vertical levels. We run the model with wind forcings that vary in horizontal and temporal resolution. We use high-resolution (1-hourly with 35 km horizontal resolution) and low-resolution winds (6-hourly with 250 km horizontal resolution). We address the following questions: Is the kinetic energy of near-inertial waves enhanced when high-resolution wind forcings are used? If so, is this due to higher level of overall wind variability or higher spatial or temporal resolution of wind forcing? How large is the power of near-inertial waves generated by winds? Our results show that near-inertial waves are enhanced and the near-inertial kinetic energy is two times higher (in the storm track regions 3.5 times higher) when high-resolution winds are used. Filtering high-resolution winds in space and time, the near-inertial kinetic energy reduces. The reduction is faster when a temporal filter is used suggesting that the high-frequency wind forcing is more efficient in generating near-inertial wave energy than the small-scale wind forcing. Using low-resolution wind forcing the wind generated power to near-inertial waves is 0.55 TW. When we use high-resolution wind forcing the result is 1.6 TW meaning that the result increases by 300%.

  11. Measuring the Kinetic and Mechanical Properties of Non-Processive Myosins using Optical Tweezers

    PubMed Central

    Greenberg, Michael J.; Shuman, Henry; Ostap, E. Michael

    2017-01-01

    The myosin superfamily of molecular motors utilizes energy from ATP hydrolysis to generate force and motility along actin filaments in a diverse array of cellular processes. These motors are structurally, kinetically, and mechanically tuned to their specific molecular roles in the cell. Optical trapping techniques have played a central role in elucidating the mechanisms by which myosins generate force and in exposing the remarkable diversity of myosin functions. Here, we present thorough methods for measuring and analyzing interactions between actin and non-processive myosins using optical trapping techniques. PMID:27844441

  12. The amazing cases of motion with friction

    NASA Astrophysics Data System (ADS)

    Grech, Dariusz; Mazur, Zygmunt

    2001-07-01

    The paper describes the behaviour of a simple mechanical system, which should help students (or teachers) to understand and clarify the importance of relative motion of two surfaces when kinetic friction is present. We show that despite the simplicity of this system, the peculiar interplay between friction forces, tension forces and gravity leads to physical solutions exceeding in many cases most intuitive expectations. These are discussed in detail. The problem is intended to be solved in a theoretical framework as an example, which helps to understand better the physical background of kinetic friction phenomena.

  13. Comparison of quasi-static and dynamic squats: a three-dimensional kinematic, kinetic and electromyographic study of the lower limbs.

    PubMed

    Clément, Julien; Hagemeister, Nicola; Aissaoui, Rachid; de Guise, Jacques A

    2014-01-01

    Numerous studies have described 3D kinematics, 3D kinetics and electromyography (EMG) of the lower limbs during quasi-static or dynamic squatting activities. One study compared these two squatting conditions but only at low speed on healthy subjects, and provided no information on kinetics and EMG of the lower limbs. The purpose of the present study was to contrast simultaneous recordings of 3D kinematics, 3D kinetics and EMG of the lower limbs during quasi-stat ic and fast-dynamic squats in healthy and pathological subjects. Ten subjects were recruited: five healthy and five osteoarthritis subjects. A motion-capture system, force plate, and surface electrodes respectively recorded 3D kinematics, 3D kinetics and EMG of the lower limbs. Each subject performed a quasi-static squat and several fast-dynamic squats from 0° to 70° of knee flexion. The two squatting conditions were compared for positions where quasi-static and fast-dynamic knee flexion-extension angles were similar. Mean differences between quasi-static and fast-dynamic squats were 1.5° for rotations, 1.9 mm for translations, 2.1% of subjects' body weight for ground reaction forces, 6.6 Nm for torques, 11.2 mm for center of pressure, and 6.3% of maximum fast-dynamic electromyographic activities for EMG. Some significant differences (p<0.05) were found in internal rotation, anterior translation, vertical force and EMG. All differences between quasi-static and fast-dynamic squats were small. 69.5% of compared data were equivalent. In conclusion, this study showed that quasi-static and fast-dynamic squatting activities are comparable in terms of 3D kinematics, 3D kinetics and EMG, although some reservations still remain. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. 2-Deoxyadenosine triphosphate restores the contractile function of cardiac myofibril from adult dogs with naturally occurring dilated cardiomyopathy

    PubMed Central

    Cheng, Yuanhua; Hogarth, Kaley A.; O'Sullivan, M. Lynne; Regnier, Michael

    2015-01-01

    Dilated cardiomyopathy (DCM) is a major type of heart failure resulting from loss of systolic function. Naturally occurring canine DCM is a widely accepted experimental paradigm for studying human DCM. 2-Deoxyadenosine triphosphate (dATP) can be used by myosin and is a superior energy substrate over ATP for cross-bridge formation and increased systolic function. The objective of this study was to evaluate the beneficial effect of dATP on contractile function of cardiac myofibrils from dogs with naturally occurring DCM. We measured actomyosin NTPase activity and contraction/relaxation properties of isolated myofibrils from nonfailing (NF) and DCM canine hearts. NTPase assays indicated replacement of ATP with dATP significantly increased myofilament activity in both NF and DCM samples. dATP significantly improved maximal tension of DCM myofibrils to the NF sample level. dATP also restored Ca2+ sensitivity of tension that was reduced in DCM samples. Similarly, dATP increased the kinetics of contractile activation (kACT), with no impact on the rate of cross-bridge tension redevelopment (kTR). Thus, the activation kinetics (kACT/kTR) that were reduced in DCM samples were restored for dATP to NF sample levels. dATP had little effect on relaxation. The rate of early slow-phase relaxation was slightly reduced with dATP, but its duration was not, nor was the fast-phase relaxation or times to 50 and 90% relaxation. Our findings suggest that myosin utilization of dATP improves cardiac myofibril contractile properties of naturally occurring DCM canine samples, restoring them to NF levels, without compromising relaxation. This suggests elevation of cardiac dATP is a promising approach for the treatment of DCM. PMID:26497964

  15. 2-Deoxyadenosine triphosphate restores the contractile function of cardiac myofibril from adult dogs with naturally occurring dilated cardiomyopathy.

    PubMed

    Cheng, Yuanhua; Hogarth, Kaley A; O'Sullivan, M Lynne; Regnier, Michael; Pyle, W Glen

    2016-01-01

    Dilated cardiomyopathy (DCM) is a major type of heart failure resulting from loss of systolic function. Naturally occurring canine DCM is a widely accepted experimental paradigm for studying human DCM. 2-Deoxyadenosine triphosphate (dATP) can be used by myosin and is a superior energy substrate over ATP for cross-bridge formation and increased systolic function. The objective of this study was to evaluate the beneficial effect of dATP on contractile function of cardiac myofibrils from dogs with naturally occurring DCM. We measured actomyosin NTPase activity and contraction/relaxation properties of isolated myofibrils from nonfailing (NF) and DCM canine hearts. NTPase assays indicated replacement of ATP with dATP significantly increased myofilament activity in both NF and DCM samples. dATP significantly improved maximal tension of DCM myofibrils to the NF sample level. dATP also restored Ca(2+) sensitivity of tension that was reduced in DCM samples. Similarly, dATP increased the kinetics of contractile activation (kACT), with no impact on the rate of cross-bridge tension redevelopment (kTR). Thus, the activation kinetics (kACT/kTR) that were reduced in DCM samples were restored for dATP to NF sample levels. dATP had little effect on relaxation. The rate of early slow-phase relaxation was slightly reduced with dATP, but its duration was not, nor was the fast-phase relaxation or times to 50 and 90% relaxation. Our findings suggest that myosin utilization of dATP improves cardiac myofibril contractile properties of naturally occurring DCM canine samples, restoring them to NF levels, without compromising relaxation. This suggests elevation of cardiac dATP is a promising approach for the treatment of DCM. Copyright © 2016 the American Physiological Society.

  16. 24 CFR 598.215 - What are the purpose and content of the strategic plan?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... preserve the environment and historic landmarks, address “brownfields” clean-up and redevelopment, explore..., Environmental Cleanup Cost Deduction (i.e., “Brownfields Tax Incentive”), and the Work Opportunity Tax Credit...

  17. 24 CFR 598.215 - What are the purpose and content of the strategic plan?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... preserve the environment and historic landmarks, address “brownfields” clean-up and redevelopment, explore..., Environmental Cleanup Cost Deduction (i.e., “Brownfields Tax Incentive”), and the Work Opportunity Tax Credit...

  18. 24 CFR 598.215 - What are the purpose and content of the strategic plan?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... preserve the environment and historic landmarks, address “brownfields” clean-up and redevelopment, explore..., Environmental Cleanup Cost Deduction (i.e., “Brownfields Tax Incentive”), and the Work Opportunity Tax Credit...

  19. In-Depth Case Studies of Superfund Reuse

    EPA Pesticide Factsheets

    SRI’s in-depth case studies explore Superfund reuse stories from start to finish. Their purpose is to see what redevelopment strategies worked, acknowledge reuse barriers and understand how communities overcame the barriers to create new reuse outcomes.

  20. Unlocking Brownfields Redevelopment: Establishing a Local Revolving Loan Fund Program

    EPA Pesticide Factsheets

    This Brownfields Solutions Series fact sheet is intended to provide an overview to those interested in applying for an RLF grant, and information to new RLF grantees on how to establish an RLF program.

  1. Canton hydroelectric project: feasibility study. Final report, appendices

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1979-05-01

    These appendices contain legal, environmental, regulatory, technical and economic information used in evaluating the feasibility of redeveloping the hydroelectric power generating facilities at the Upper and Lower Dams of the Farmington River at Collinsville, CT. (LCL)

  2. Cognitive agents and pedestrian-oriented redevelopment.

    DOT National Transportation Integrated Search

    2016-11-01

    Walking is one of the most commonplace forms of human expressions, yet the : forms, motivations, and practices of walking vary greatly and are often at odds with : dominant discourses in urban and transportation planning. As interest in pedestrianori...

  3. 77 FR 65534 - Magnuson-Stevens Act Provisions; General Provisions for Domestic Fisheries; Application for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-29

    ... Sustainable Fisheries, Northeast Region, NMFS, has made a preliminary determination that the subject exempted... Network to Redevelop a Sustainable Redfish (Sebastes fasciatus) Trawl Fishery in the Gulf of Maine... [[Page 65535

  4. Brownfields New Markets Tax Credits

    EPA Pesticide Factsheets

    This Brownfi elds Solutions factsheet is intended for brownfields stakeholders interested in how the U.S. Department of the Treasury’s New Markets Tax Credit (NMTC) Program can be used as a financing mechanism in brownfields cleanup and redevelopment.

  5. Assessing soil and groundwater contamination in a metropolitan redevelopment project.

    PubMed

    Yun, Junki; Lee, Ju Young; Khim, Jeehyeong; Ji, Won Hyun

    2013-08-01

    The purpose of this study was to assess contaminated soil and groundwater for the urban redevelopment of a rapid transit railway and a new mega-shopping area. Contaminated soil and groundwater may interfere with the progress of this project, and residents and shoppers may be exposed to human health risks. The study area has been remediated after application of first remediation technologies. Of the entire area, several sites were still contaminated by waste materials and petroleum. For zinc (Zn) contamination, high Zn concentrations were detected because waste materials were disposed in the entire area. For petroleum contamination, high total petroleum hydrocarbon (TPH) and hydrocarbon degrading microbe concentrations were observed at the depth of 7 m because the underground petroleum storage tank had previously been located at this site. Correlation results suggest that TPH (soil) concentration is still related with TPH (groundwater) concentration. The relationship is taken into account in the Spearman coefficient (α).

  6. Risk management: Reducing brownfield cleanup costs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Graves, N.

    Balancing environmental protection with economic vitality is crucial to maintaining competitiveness in world markets. One key initiative that has been identified as important to both environmental protection and the economy is the redevelopment of brownfields. Brownfield redevelopment can stimulate local economies that have been devastated by lost jobs and can recycle industrial land use, thereby preserving undeveloped lands. Many existing brownfield sites appear on the US Environmental Protection Agency`s (EPA) National Priority List (NPL), which designates over 1200 sites and is expected to grow to more than 2000 by the end of the decade. EPA estimates the cost of remediatingmore » the sites on the current list will approach $30 billion, with the average cost of remediating a site close to $25 million. Thousands of additional brownfield sites that do not appear on the NPL are listed under state cleanup programs.« less

  7. Participatory and evidence-based recommendations for urban redevelopment following natural disasters: older adults as policy advisers.

    PubMed

    Annear, Michael; Keeling, Sally; Wilkinson, Tim

    2014-03-01

    To develop community-generated recommendations to inform urban environmental remediation following earthquakes in Christchurch, New Zealand, and share these with local decision-makers during a participatory action research process. This study employed three focus group discussions to critique mixed-methods and multiphase results and develop evidence-based recommendations. Participants included 30 volunteers and 8 knowledgeable advisers aged 65 years and older. Participant recommendations addressed the remediation of earthquake-affected suburbs, access to transportation, age-friendly design, safer communities, resilient support agencies, and restoration of resources for social and cultural activities. Older collaborators identified salient barriers to active ageing and options for post-earthquake redevelopment that had not previously been considered in research or policy. Independently living older adults are well placed to work with researchers to develop recommendations to improve the urban environment following natural disasters as well as in times of relative stability. © 2013 ACOTA.

  8. Selected Black-Coal Mine Waste Dumps in the Ostrava-Karviná Region: An Analysis of Their Potential Use

    NASA Astrophysics Data System (ADS)

    Niemiec, Dominik; Duraj, Miloš; Cheng, Xianfeng; Marschalko, Marian; Kubáč, Jan

    2017-12-01

    The paper aims to analyse the options for the use of selected black-coal mine waste dump bodies in the Ostrava-Karviná Region. In the Czech Republic there are approximately 70 mine waste dumps, out of which 50 are located in the Ostrava-Karviná Coal District. The issue is highly topical, particularly in the region, because the dump bodies significantly affect the landscape character of the Ostrava-Karviná Region and pose ecological risks. In such cases, their redevelopment and land reclamation are not easy either from the environmental or economic points of view. It is clear that the redevelopment of such geological environment is difficult, and it is vital to make the right decisions as for what purposes the mine waste dumps should be used. Next, it is important to take into account all the economic and environmental aspects of the locality in question.

  9. Classification of factors of development of transport infrastructure in the region

    NASA Astrophysics Data System (ADS)

    Karpovich, Oleg; Shlafman, Alexander

    2017-10-01

    The formation of a unified strategy for the development of the transport infrastructure of the region is considered to be the basic direction of the developing the whole territory The article is devoted to determining priorities in the implementation of programs of redevelopment of the regional plants. The submission suggested authors the characteristics of a grouping of theoretical and practical approaches to spatial reorganization of production. The result of this research, the specific recommendations on the application of redevelopment for the development of industrial areas. The organization of economic relations within the eastern regions of the Russian Federation, as well as the implementation of economic activities involving the transit and processing of a multitude of material and intangible flows on their territory, is a priority task of the state policy of smoothing the development of the regions of the country. To solve these macroeconomic problems, the transport infrastructure of the region has a priority.

  10. Accelerated lamellar disintegration in eutectoid steel

    NASA Astrophysics Data System (ADS)

    Mishra, Shakti; Mishra, Alok; Show, Bijay Kumar; Maity, Joydeep

    2017-04-01

    The fastest kinetics of lamellar disintegration (predicted duration of 44 min) in AISI 1080 steel is obtained with a novel approach of incomplete austenitisation-based cyclic heat treatment involving forced air cooling with an air flow rate of 8.7 m3 h-1. A physical model for process kinetics is proposed that involves lamellar fragmentation, lamellar thickening, divorced eutectoid growth and generation of new lamellar faults in remaining cementite lamellae in each cycle. Lamellar fragmentation is accentuated with faster rate of cooling through generation of more intense lamellar faults; but divorced eutectoid growth is ceased. Accordingly, as compared to still air cooling, much faster kinetics of lamellar disintegration is obtained by forced air cooling together with the generation of much smaller submicroscopic cementite particles (containing more proportion of plate-shaped non-spheroids) in divorced eutectoid region.

  11. Hydrothermal Atomic Force Microscopy Investigation of Barite Growth: Role of Spectator Ions in Elementary Step Edge Growth Kinetics and Hillock Morphology [Supporting Information Only

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jindra, Sarah A.; Bertagni, Angela L.; Bracco, Jacquelyn N.

    Here, to better understand the role of spectator ions in barite growth, the kinetics of step edge growth on barite (001) surfaces were studied under various salt solutions. Hydrothermal atomic force microscopy (HAFM) was used to investigate the effect of background electrolytes (NaCl, NaBr, and NaNO 3) as a function of saturation index and ionic strength ( I) on barite growth sourced at dislocations at 108 °C. Results demonstrate that hillock morphology is affected by I, as well as type of anion, where the prevalence of steps aligned on the [010] direction is highest under Cl –. There is amore » modest increase in kinetic coefficient of 55–130% with a 10-fold increase in I for each salt. In comparing the kinetic coefficients of the salts at low ionic strength (0.01 M), there is a moderate difference, suggesting that the anion may play a role in barium attachment.« less

  12. 2001 NRL Review

    DTIC Science & Technology

    2001-01-01

    Air Force Research Laboratory , Rome Research Site ( AFRL /RSS) in Rome, New York. The... AFRL ). The Kinetic Kill Vehicle Hardware in the Loop Simulator (KHILS) is managed by the Air Force Research Laboratory Mu- nitions Directorate ( AFRL ...Development Department 2Spacecraft Engineering Department 3Optical Sciences Division 4U.S. Air Force Research Laboratory 5SAIC Introduction:

  13. Force spectroscopy of multivalent binding of riboflavin-conjugated dendrimers to riboflavin binding protein.

    PubMed

    Leistra, Abigail N; Han, Jong Hyun; Tang, Shengzhuang; Orr, Bradford G; Banaszak Holl, Mark M; Choi, Seok Ki; Sinniah, Kumar

    2015-05-07

    Putative riboflavin receptors are considered as biomarkers due to their overexpression in breast and prostate cancers. Hence, these receptors can be potentially exploited for use in targeted drug delivery systems where dendrimer nanoparticles with multivalent ligand attachments can lead to greater specificity in cellular interactions. In this study, the single molecule force spectroscopy technique was used to assess the physical strength of multivalent interactions by employing a riboflavin (RF)-conjugated generation 5 PAMAM dendrimer G5(RF)n nanoparticle. By varying the average RF ligand valency (n = 0, 3, 5), the rupture force was measured between G5(RF)n and the riboflavin binding protein (RFBP). The rupture force increased when the valency of RF increased. We observed at the higher valency (n = 5) three binding events that increased in rupture force with increasing loading rate. Assuming a single energy barrier, the Bell-Evans model was used to determine the kinetic off-rate and barrier width for all binding interactions. The analysis of our results appears to indicate that multivalent interactions are resulting in changes to rupture force and kinetic off-rates.

  14. Ultrafast single molecule technique for the study of force dependent kinetics and conformational changes of actin-protein interaction involved in mechanotransduction

    NASA Astrophysics Data System (ADS)

    Sergides, M.; Arbore, C.; Pavone, F. S.; Capitanio, M.

    2018-02-01

    Mechanical signals occurring at the interface between cell membrane and extracellular matrix and at intercellular junctions trigger biochemical signals that are fundamental for cell growth, development and regulation. Adaptor proteins, which link the cell membrane to the actin cytoskeleton, seem to partake in this process of mechanotransduction. In particular, catenins play a key role in intercellular junctions, where they act as a bridge between the cell membrane and actin. Studies suggest that α-catenin contains a domain that normally masks vinculin binding sites, which can become accessible after a conformational change induced by an external force. Here we demonstrate a single-molecule technique for investigating actin-protein interactions at different forces (up to 17 pN) with adequate temporal resolution (sub-ms). This system is based on the ultrafast force-clamp spectroscopy technique that has been recently developed by our group and is adapted to study and measure force-dependent kinetics of the catenin-actin interaction, as well as the amplitude of the expected conformational changes such as force-induced protein unfolding.

  15. Lower extremity kinetics in tap dance.

    PubMed

    Mayers, Lester; Bronner, Shaw; Agraharasamakulam, Sujani; Ojofeitimi, Sheyi

    2010-01-01

    Tap dance is a unique performing art utilizing the lower extremities as percussion instruments. In a previous study these authors reported decreased injury prevalence among tap dancers compared to other dance and sports participants. No biomechanical analyses of tap dance exist to explain this finding. The purpose of the current pilot study was to provide a preliminary overview of normative peak kinetic and kinematic data, based on the hypothesis that tap dance generates relatively low ground reaction forces and joint forces and moments. Six professional tap dancers performed four common tap dance sequences that produced data captured by the use of a force platform and a five-camera motion analysis system. The mean vertical ground reaction force for all sequences was found to be 2.06+/-0.55 BW. Mean peak sagittal, frontal, and transverse plane joint moments (hip, knee, and ankle) ranged from 0.07 to 2.62 N.m/kg. These small ground reaction forces and joint forces and moments support our hypothesis, and may explain the relatively low injury incidence in tap dancers. Nevertheless, the analysis is highly complex, and other factors remain to be studied and clarified.

  16. Computing fluid-particle interaction forces for nano-suspension droplet spreading: molecular dynamics simulations

    NASA Astrophysics Data System (ADS)

    Zhou, Weizhou; Shi, Baiou; Webb, Edmund

    2017-11-01

    Recently, there are many experimental and theoretical studies to understand and control the dynamic spreading of nano-suspension droplets on solid surfaces. However, fundamental understanding of driving forces dictating the kinetics of nano-suspension wetting and spreading, especially capillary forces that manifest during the process, is lacking. Here, we present results from atomic scale simulations that were used to compute forces between suspended particles and advancing liquid fronts. The role of nano-particle size, particle loading, and interaction strength on forces computed from simulations will be discussed. Results demonstrate that increasing the particle size dramatically changes observed wetting behavior from depinning to pinning. From simulations on varying particle size, a relationship between computed forces and particle size is advanced and compared to existing expressions in the literature. High particle loading significantly slowed spreading kinetics, by introducing tortuous transport paths for liquid delivery to the advancing contact line. Lastly, we show how weakening the interaction between the particle and the underlying substrate can change a system from exhibiting pinning behavior to de-pinning.

  17. Domain Growth Kinetics in Stratifying Foam Films

    NASA Astrophysics Data System (ADS)

    Zhang, Yiran; Sharma, Vivek

    2015-03-01

    Baking bread, brewing cappuccino, pouring beer, washing dishes, shaving, shampooing, whipping eggs and blowing bubbles all involve creation of aqueous foam films. Typical foam films consist of two surfactant-laden surfaces that are μ 5 nm - 10 micron apart. Sandwiched between these interfacial layers is a fluid that drains primarily under the influence of viscous and interfacial forces, including disjoining pressure. Interestingly, for certain low molecular weight surfactants, a layered ordering of micelles inside the foam films (thickness <100 nm) leads to a stepwise thinning phenomena called stratification. We experimentally elucidate the influence of these different driving forces, and confinement on drainage kinetics of horizontal stratifying foam films. Thinner, darker domains spontaneously grow within foam films. Quantitative characterization of domain growth visualized in a using Scheludko-type thin film cell and a theoretical model based on lubrication analysis, provide critical insights into hydrodynamics of thin foam films, and the strength and nature of surface forces, including supramolecular oscillatory structural forces.

  18. Returning property to the tax rolls, a case study

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Aveggio, J.

    1995-09-01

    A major deterrent to the redevelopment of intercity areas is the high cost associated with mitigating residual soil and groundwater contamination resulting from years of industrial activities. If the properties remain undeveloped, their revenue to the local taxing authority remains at minimal levels. It is not unreasonable to assume that a redeveloped property would increase 10 to 100 times in assessed value. In California, the local taxing authority bases its tax assessment as a percentage of the assessed value. Therefore, it is in the taxing authority`s best interest to encourage and provide incentives for redevelopment. The City of Eureka andmore » Price-Costco combined to remediate a contaminated property, build a Costco store, provide jobs, and return a property to the tax rolls. The effort was accomplished through the negotiation of site specific cleanup levels for petroleum hydrocarbons and remediation of approximately 16,000 tons of soil by thermal desorption. Site specific cleanup levels were established by using a leaching procedure to establish the contaminant concentration in soil that would impact groundwater, and through an economic analysis of cleanup level versus benefit. Petroleum contaminated soil was excavated from 11 sources areas and transported to an on-site thermal desorber for treatment. The soil contained the full spectrum of petroleum hydrocarbons, from gasoline to heavy oils. The thermal desorber was able to consistently treat this wide variety of contamination to nondetectable levels. Following treatment, the soil was backfilled and compacted into the excavations. The entire cleanup was complete in approximately 2 months and was performed concurrently with the construction of the store.« less

  19. 40 shades of black: regional differences in vegetation response to a changing human influence in the Low Countries during the Dark Ages (AD 300-1000).

    NASA Astrophysics Data System (ADS)

    Gouw-Bouman, Marjolein T. I. J.; Donders, Timme H.; Hoek, Wim Z.

    2016-04-01

    During the Dark Ages, which includes the Late Roman Period (LRP, AD 300-500) and the Early Middle Ages (EMA, AD 500-1000), large scale vegetation development in Northwestern Europe is generally characterized by a forest regeneration. This forest redevelopment phase was not uniformous across the Netherlands. A comparison between existing pollen records shows that forest redevelopment started earlier and was more severe in the southern part of the Netherlands than in the northeastern Netherlands. The prevailing view advocates that the forest redevelopment is the result of a diminishing human influence on the landscape due to the collapse of the Roman Empire. Following this view, regional changes in forest regeneration are explained by varying population densities. However, existing climate-records indicate a colder and wetter climate during the Dark Ages and the geomorphological record points to a changing landscape. How and to what extent these climatic and environmental changes contributed to the changes in vegetation development or even to the decline of the Roman Empire is largely unknown. To understand the relative importance of the factors (climate, environment, economy and demography) influencing vegetation development it is important to accurately map regional differences in vegetation both on a regional and extra-regional scale. For an extra-regional overview all available pollen records in the Netherlands from this period are compiled to show differences in amplitude of the vegetation development during the Dark Ages. On a regional scale, vegetation reconstruction maps have been produced reflecting the influence of geological/geomorphological factors.

  20. Nucleation theory with delayed interactions: An application to the early stages of the receptor-mediated adhesion/fusion kinetics of lipid vesicles

    NASA Astrophysics Data System (ADS)

    Raudino, Antonio; Pannuzzo, Martina

    2010-01-01

    A semiquantitative theory aimed to describe the adhesion kinetics between soft objects, such as living cells or vesicles, has been developed. When rigid bodies are considered, the adhesion kinetics is successfully described by the classical Derjaguin, Landau, Verwey, and Overbeek (DLVO) picture, where the energy profile of two approaching bodies is given by a two asymmetrical potential wells separated by a barrier. The transition probability from the long-distance to the short-distance minimum defines the adhesion rate. Conversely, soft bodies might follow a different pathway to reach the short-distance minimum: thermally excited fluctuations give rise to local protrusions connecting the approaching bodies. These transient adhesion sites are stabilized by short-range adhesion forces (e.g., ligand-receptor interactions between membranes brought at contact distance), while they are destabilized both by repulsive forces and by the elastic deformation energy. Above a critical area of the contact site, the adhesion forces prevail: the contact site grows in size until the complete adhesion of the two bodies inside a short-distance minimum is attained. This nucleation mechanism has been developed in the framework of a nonequilibrium Fokker-Planck picture by considering both the adhesive patch growth and dissolution processes. In addition, we also investigated the effect of the ligand-receptor pairing kinetics at the adhesion site in the time course of the patch expansion. The ratio between the ligand-receptor pairing kinetics and the expansion rate of the adhesion site is of paramount relevance in determining the overall nucleation rate. The theory enables one to self-consistently include both thermodynamics (energy barrier height) and dynamic (viscosity) parameters, giving rise in some limiting cases to simple analytical formulas. The model could be employed to rationalize fusion kinetics between vesicles, provided the short-range adhesion transition is the rate-limiting step to the whole adhesion process. Approximate relationships between the experimental fusion rates reported in the literature and parameters such as membrane elastic bending modulus, repulsion strength, temperature, osmotic forces, ligand-receptor binding energy, solvent and membrane viscosities are satisfactory explained by our model. The present results hint a possible role of the initial long-distance→short-distance transition in determining the whole fusion kinetics.

  1. Nucleation theory with delayed interactions: an application to the early stages of the receptor-mediated adhesion/fusion kinetics of lipid vesicles.

    PubMed

    Raudino, Antonio; Pannuzzo, Martina

    2010-01-28

    A semiquantitative theory aimed to describe the adhesion kinetics between soft objects, such as living cells or vesicles, has been developed. When rigid bodies are considered, the adhesion kinetics is successfully described by the classical Derjaguin, Landau, Verwey, and Overbeek (DLVO) picture, where the energy profile of two approaching bodies is given by a two asymmetrical potential wells separated by a barrier. The transition probability from the long-distance to the short-distance minimum defines the adhesion rate. Conversely, soft bodies might follow a different pathway to reach the short-distance minimum: thermally excited fluctuations give rise to local protrusions connecting the approaching bodies. These transient adhesion sites are stabilized by short-range adhesion forces (e.g., ligand-receptor interactions between membranes brought at contact distance), while they are destabilized both by repulsive forces and by the elastic deformation energy. Above a critical area of the contact site, the adhesion forces prevail: the contact site grows in size until the complete adhesion of the two bodies inside a short-distance minimum is attained. This nucleation mechanism has been developed in the framework of a nonequilibrium Fokker-Planck picture by considering both the adhesive patch growth and dissolution processes. In addition, we also investigated the effect of the ligand-receptor pairing kinetics at the adhesion site in the time course of the patch expansion. The ratio between the ligand-receptor pairing kinetics and the expansion rate of the adhesion site is of paramount relevance in determining the overall nucleation rate. The theory enables one to self-consistently include both thermodynamics (energy barrier height) and dynamic (viscosity) parameters, giving rise in some limiting cases to simple analytical formulas. The model could be employed to rationalize fusion kinetics between vesicles, provided the short-range adhesion transition is the rate-limiting step to the whole adhesion process. Approximate relationships between the experimental fusion rates reported in the literature and parameters such as membrane elastic bending modulus, repulsion strength, temperature, osmotic forces, ligand-receptor binding energy, solvent and membrane viscosities are satisfactory explained by our model. The present results hint a possible role of the initial long-distance-->short-distance transition in determining the whole fusion kinetics.

  2. Impact of short range hydrophobic interactions and long range electrostatic forces on the aggregation kinetics of a monoclonal antibody and a dual-variable domain immunoglobulin at low and high concentrations.

    PubMed

    Kumar, Vineet; Dixit, Nitin; Zhou, Liqiang Lisa; Fraunhofer, Wolfgang

    2011-12-12

    The purpose of this work was to determine the nature of long and short-range forces governing protein aggregation kinetics at low and high concentrations for a monoclonal antibody (IgG1) and a dual-variable-domain immunoglobulin (DVD-Ig). Protein-protein interactions (PPI) were studied under dilute conditions by utilizing the methods of static (B(22)) and dynamic light scattering (k(D)). PPI in solutions containing minimal ionic strengths were characterized to get detailed insights into the impact of ionic strength on aggregation. Microcalorimetry and susceptibility to denature at air-liquid interface were used to assess the tertiary structure and quiescent stability studies were conducted to study aggregation characteristics. Results for IgG1 showed that electrostatic interactions governed protein aggregation kinetics both under dilute and concentrated conditions (i.e., 5 mg/mL and 150 mg/mL). For DVD-Ig molecules, on the other hand, although electrostatic interactions governed protein aggregation under dilute conditions, hydrophobic forces clearly determined the kinetics at high concentrations. This manuscript shows for the first time that short-range hydrophobic interactions can outweigh electrostatic forces and play an important role in determining protein aggregation at high concentrations. Additionally, results show that although higher-order virial coefficients become significant under low ionic strength conditions, removal of added charges may be used to enhance the aggregation stability of dilute protein formulations. Copyright © 2011 Elsevier B.V. All rights reserved.

  3. Comparison of friction force between corroded and noncorroded titanium nitride plating of metal brackets.

    PubMed

    Kao, Chia-Tze; Guo, Jia-Uei; Huang, Tsui-Hsien

    2011-05-01

    Titanium nitride (TiN) plating is a method to prevent metal corrosion and can increase the surface smoothness. The purpose of this study was to evaluate the friction forces between the orthodontic bracket, with or without TiN plating, and stainless steel wire after it was corroded in fluoride-containing solution. In total, 540 metal brackets were divided into a control group and a TiN-coated experimental group. The electrochemical corrosion was performed in artificial saliva with 1.23% acidulated phosphate fluoride (APF) as the electrolytes. Static and kinetic friction were measured by an EZ-test machine (Shimadazu, Tokyo, Japan) with a crosshead speed of 10 mm per minute over a 5-mm stretch of stainless steel archwire. The data were analyzed by using unpaired t test and analysis of variance (ANOVA). Both the control and TiN-coated groups' corrosion potential was higher with 1.23% APF solution than with artificial solution (P <0.05). In brackets without corrosion, both the static and kinetic friction force between the control and TiN-coated brackets groups showed a statistically significant difference (P <0.05). In brackets with corrosion, the control group showed no statistical difference on kinetic or static friction. The TiN-coated brackets showed a statistical difference (P <0.05) on kinetic and static friction in different solutions. TiN-coated metal brackets, with corrosion or without corrosion, cannot reduce the frictional force. Copyright © 2011 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

  4. 76 FR 56271 - Notice of Release From Federal Grant Assurance Obligations for Livermore Municipal Airport...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-12

    ... acquired parcel will be redeveloped with a hydromodification basin for flood control and to reduce water..., and provided continued protection of its approach surfaces. The reuse of the released parcel for...

  5. SMARTE: IMPROVING REVITALIZATION DECISIONS FOR KANSAS COMMUNITIES

    EPA Science Inventory

    The Kansas Department of Health and Environment (KDHE) Brownfields program helps local governments (city/county) and non-profit organizations appropriately assess, clean up, and reuse contaminated properties that they own or purchase. Reuse and redevelopment of abandoned, idle, o...

  6. TECHNICAL APPROACHES TO CHARACTERIZING AND CLEANUP OF AUTOMOTIVE RECYCLING BROWNFIELDS

    EPA Science Inventory

    The guidance document gives assistance to communities, decision-makers, states and municipalities, academia, and the private sector to address issues related to the redevelopment of Brownfields sites, specifically automotive recycling sites. The document helps users to understand...

  7. Managing Vacant and Abandoned Property in the Green Zone of Saginaw, Michigan

    EPA Pesticide Factsheets

    Report from a technical assistance project with Saginaw, MI, gives the city options to consider to stabilize neighborhoods through sustainable reuse of abandoned properties, including redeveloping them as green infrastructure, and to create opportunities.

  8. Brownfields Waterfront Sustainability Pilot, Allentown PA: Technical Memorandum on Conceptual Design Using Low Impact Development

    EPA Pesticide Factsheets

    This technical memorandum briefly describes the site and the master plan, indicates design constraints considered, specifies recommended LID stormwater techniques and features for sustainable redevelopment of the site, and offers other recommendations.

  9. THE USE OF SPATIAL ANALYTICAL TECHNIQUES TO IDENTIFY POTENTIAL BROWNFIELDS SITES

    EPA Science Inventory

    Brownfields are abandoned, idled, or underutilized properties where expansion or redevelopment is complicated by real or perceived environmental contamination. Most Brownfields sites are located in urban, commercial, and industrial areas. Under the Brownfields Program, the United...

  10. Brownfields Samoa Peninsula Project: Phase I Sustainable Site Analysis Final Report

    EPA Pesticide Factsheets

    This report provides an analysis and scoring using the Leadership in Energy and Environmental Design, Neighborhood Development Rating System, and the Land and Natural Development Code in order to assess the proposed redevelopment a master plan.

  11. Missouri River freight corridor assessment & development plan.

    DOT National Transportation Integrated Search

    2011-09-01

    The purpose of the Missouri River Freight Corridor Development effort is to redevelop the river as a freight corridor with logical market nodes and reliable service that supports a sustainable market and logistics system. Four focus areas have been i...

  12. 24 CFR 586.5 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMMUNITY FACILITIES REVITALIZING BASE CLOSURE COMMUNITIES AND COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT... Response, Compensation, and Liability Act (42 U.S.C. 9601 et seq.). Communities in the vicinity of the...

  13. A flexible object-oriented software framework for developing complex multimedia simulations.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sydelko, P. J.; Dolph, J. E.; Christiansen, J. H.

    Decision makers involved in brownfields redevelopment and long-term stewardship must consider environmental conditions, future-use potential, site ownership, area infrastructure, funding resources, cost recovery, regulations, risk and liability management, community relations, and expected return on investment in a comprehensive and integrated fashion to achieve desired results. Successful brownfields redevelopment requires the ability to assess the impacts of redevelopment options on multiple interrelated aspects of the ecosystem, both natural and societal. Computer-based tools, such as simulation models, databases, and geographical information systems (GISs) can be used to address brownfields planning and project execution. The transparent integration of these tools into a comprehensivemore » and dynamic decision support system would greatly enhance the brownfields assessment process. Such a system needs to be able to adapt to shifting and expanding analytical requirements and contexts. The Dynamic Information Architecture System (DIAS) is a flexible, extensible, object-oriented framework for developing and maintaining complex multidisciplinary simulations of a wide variety of application domains. The modeling domain of a specific DIAS-based simulation is determined by (1) software objects that represent the real-world entities that comprise the problem space (atmosphere, watershed, human), and (2) simulation models and other data processing applications that express the dynamic behaviors of the domain entities. Models and applications used to express dynamic behaviors can be either internal or external to DIAS, including existing legacy models written in various languages (FORTRAN, C, etc.). The flexible design framework of DIAS makes the objects adjustable to the context of the problem without a great deal of recoding. The DIAS Spatial Data Set facility allows parameters to vary spatially depending on the simulation context according to any of a number of 1-D, 2-D, or 3-D topologies. DIAS is also capable of interacting with other GIS packages and can import many standard spatial data formats. DIAS simulation capabilities can also be extended by including societal process models. Models that implement societal behaviors of individuals and organizations within larger DIAS-based natural systems simulations allow for interaction and feedback among natural and societal processes. The ability to simulate the complex interplay of multimedia processes makes DIAS a promising tool for constructing applications for comprehensive community planning, including the assessment of multiple development and redevelopment scenarios.« less

  14. Three Essays In and Tests of Theoretical Urban Economics

    NASA Astrophysics Data System (ADS)

    Zhao, Weihua

    This dissertation consists of three essays on urban economics. The three essays are related to urban spatial structure change, energy consumption, greenhouse gas emissions, and housing redevelopment. Chapter 1 answers the question: Does the classic Standard Urban Model still describe the growth of cities? Chapter 2 derives the implications of telework on urban spatial structure, energy consumption, and greenhouse gas emissions. Chapter 3 investigates the long run effects of minimum lot size zoning on neighborhood redevelopment. Chapter 1 identifies a new implication of the classic Standard Urban Model, the "unitary elasticity property (UEP)", which is the sum of the elasticity of central density and the elasticity of land area with respect to population change is approximately equal to unity. When this implication of the SUM is tested, it fits US cities fairly well. Further analysis demonstrates that topographic barriers and age of housing stock are the key factors explaining deviation from the UEP. Chapter 2 develops a numerical urban simulation model with households that are able to telework to investigate the urban form, congestion, energy consumption and greenhouse gas emission implications of telework. Simulation results suggest that by reducing transportation costs, telework causes sprawl, with associated longer commutes and consumption of larger homes, both of which increase energy consumption. Overall effects depend on who captures the gains from telework (workers versus firms), urban land use regulation such as height limits or greenbelts, and the fraction of workers participating in telework. The net effects of telework on energy use and GHG emissions are generally negligible. Chapter 3 applies dynamic programming to investigate the long run effects of minimum lot size zoning on neighborhood redevelopment. With numerical simulation, comparative dynamic results show that minimum lot size zoning can delay initial land conversion and slow down demolition and housing redevelopment. Initially, minimum lot size zoning is not binding. However, as city grows, it becomes binding and can effectively distort housing supply. It can lower both floor area ratio and residential density, and reduce aggregate housing supply. Overall, minimum lot size zoning can stabilize the path of structure/land ratios, housing service levels, structure density, and housing prices. In addition, minimum lot size zoning provides more incentive for developer to maintain the building, slow structure deterioration, and raise the minimum level of housing services provided over the life cycle of development.

  15. The Kinetics of Swinging a Baseball Bat.

    PubMed

    Crisco, Joseph J; Osvalds, Nikolas J; Rainbow, Michael J

    2018-04-13

    The purpose of this study was to compute the three-dimensional kinetics required to swing three youth baseball bats of varying moments of inertia (MOI). 306 swings by 22 male players (13-18 yrs.) were analyzed. Inverse dynamics with respect to the batter's hands were computed given the known kinematics and physical properties of the bats. We found that peak force increased with larger bat MOI and was strongly correlated with bat tip speed. In contrast, peak moments were weakly correlated with bat MOI and bat tip speed. Throughout the swing, the force applied to the bat was dominated by a component aligned with the long axis of the bat and directed away from the bat knob, while the moment applied to the bat was minimal until just prior to ball impact. These results indicate that players act to mostly "pull" the bat during their swing until just prior to ball impact, at which point they rapidly increase the moment on the bat. This kinetic analysis provides novel insight into the forces and moments used to swing baseball bats.

  16. The definition of turbulence and the direction of the turbulence energy cascade

    NASA Astrophysics Data System (ADS)

    Gibson, Carl

    2013-11-01

    Turbulence is defined as an eddy-like state of fluid motion where the inertial-vortex forces of the eddies are larger than any other forces that tend to damp the eddies out. Because vorticity is produced at the Kolmogorov scale, turbulent kinetic energy always cascades from small scales to large. Irrotational flows that supply kinetic energy to turbulence from large scale motions are by definition non-turbulent. The Taylor-Reynolds-Lumley cascade of kinetic energy from large scales to small is therefore a non-turbulent cascade. The Reynolds turbulence poem must be revised to avoid further confusion. Little whorls on vortex sheets, merge and pair with more of, whorls that grow by vortex forces, Slava Kolmogorov! Turbulent mixing and transport processes in natural fluids depend on fossil turbulence and fossil turbulence waves, which are impossible by the TRL cascade direction. Standard models of cosmology, astronomy, oceanography, and atmospheric transport of heat, mass, momentum and chemical species must be revised. See journalofcosmology.com Volumes 21 and 22 for oceanographic and astro-biological examples.

  17. The influence of foot position on scrum kinetics during machine scrummaging.

    PubMed

    Bayne, Helen; Kat, Cor-Jacques

    2018-05-23

    The purpose of this study was to investigate the effect of variations in the alignment of the feet on scrum kinetics during machine scrummaging. Twenty nine rugby forwards from amateur-level teams completed maximal scrum efforts against an instrumented scrum machine, with the feet in parallel and non-parallel positions. Three-dimensional forces, the moment about the vertical axis and sagittal plane joint angles were measured during the sustained pushing phase. There was a decrease in the magnitude of the resultant force and compression force in both of the non-parallel conditions compared to parallel and larger compression forces were associated with more extended hip and knee angles. Scrummaging with the left foot forward resulted in the lateral force being directed more towards the left and the turning moment becoming more clockwise. These directional changes were reversed when scrummaging with the right foot forward. Scrummaging with the right foot positioned ahead of the left may serve to counteract the natural clockwise wheel of the live scrum and could be used to achieve an anti-clockwise rotation of the scrum for tactical reasons. However, this would be associated with lower resultant forces and a greater lateral shear force component directed towards the right.

  18. Measurement of the Turbulence Kinetic Energy Budget of a Turbulent Planar Wake Flow in Pressure Gradients

    NASA Technical Reports Server (NTRS)

    Liu, Xiao-Feng; Thomas, Flint O.; Nelson, Robert C.

    2001-01-01

    Turbulence kinetic energy (TKE) is a very important quantity for turbulence modeling and the budget of this quantity in its transport equation can provide insight into the flow physics. Turbulence kinetic energy budget measurements were conducted for a symmetric turbulent wake flow subjected to constant zero, favorable and adverse pressure gradients in year-three of research effort. The purpose of this study is to clarify the flow physics issues underlying the demonstrated influence of pressure gradient on wake development and provide experimental support for turbulence modeling. To ensure the reliability of these notoriously difficult measurements, the experimental procedure was carefully designed on the basis of an uncertainty analysis. Four different approaches, based on an isotropic turbulence assumption, a locally axisymmetric homogeneous turbulence assumption, a semi-isotropy assumption and a forced balance of the TKE equation, were applied for the estimate of the dissipation term. The pressure transport term is obtained from a forced balance of the turbulence kinetic energy equation. This report will present the results of the turbulence kinetic energy budget measurement and discuss their implication on the development of strained turbulent wakes.

  19. The effects of ramp stretches on active contractions in intact mammalian fast and slow muscle fibres.

    PubMed

    Mutungi, G; Ranatunga, K W

    2001-01-01

    The effects of a ramp stretch (amplitude <6% muscle fibre length (L0), speed < 13L0 s(-1)) on twitch tension and twitch tension re-development were examined in intact mammalian (rat) fast and slow muscle fibre bundles. The experiments were done in vitro at 20 degrees C and at an initial sarcomere length of 2.68 microm. In both fibre types, a stretch applied during the rising phase of the twitch response (including the time of stimulation) increased the re-developed twitch tension (15-35%). A stretch applied before the stimulus had little or no effect on the twitch myogram in fast muscle fibres, but it increased the twitch tension (approximately 5%) in slow muscle fibres. A similar stretch had little or no effect on tetanic tension in either muscle fibre type. In general, the results indicate that the contractile-activation mechanism may be stretch sensitive and this is particularly pronounced in slow muscle fibres. Recorded at a high sampling rate and examined at an appropriate time scale, the transitory tension response to a stretch rose in at least two phases; an initial rapid tension rise to a break (break point tension, P1a) followed by a slower tension rise (apparent P2a) to a peak reached at the end of the stretch. Plotted against stretch velocity, P1a tension increased in direct proportion to stretch velocity (viscous-like) whereas, P2a tension (calculated as peak tension minus P1a tension) increased with stretch velocity to a plateau (visco-elastic). Examined at the peak of a twitch, P1a tension had a slope (viscosity coefficient) of 1.8 kN m(-2) per L0 s(-1) in fast fibres and 4.7 kN m(-2) per L0 s(-1) in slow muscle fibres. In the same preparations, P2a tension had a relaxation time of 8 ms in the fast muscle fibres and 25 ms in the slow muscle fibres. The amplitudes of both tension components scaled with the instantaneous twitch tension in qualitatively the same way as the instantaneous fibre stiffness. These fast/slow fibre type differences probably reflect differences in their cross-bridge kinetics.

  20. Application of Detailed Chemical Kinetics to Combustion Instability Modeling

    DTIC Science & Technology

    2016-01-04

    Modeling 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) Harvazinski, Matt; Talley, Doug; Sankaran, Venke 5d. PROJECT...Chemical Kinetics to Combustion Instability Modeling Matt Harvazinski, Doug Talley, Venke Sankaran Air Force Research Laboratory Edwards AFB, CA...distribution unlimited. 3 Prior Work – Kinetics Used • Simulations : 1) 3D real geometry 2) Unsteady 3) Long run-times 4) Coupled physics • 1- 4

  1. Qualitative analysis of a discrete thermostatted kinetic framework modeling complex adaptive systems

    NASA Astrophysics Data System (ADS)

    Bianca, Carlo; Mogno, Caterina

    2018-01-01

    This paper deals with the derivation of a new discrete thermostatted kinetic framework for the modeling of complex adaptive systems subjected to external force fields (nonequilibrium system). Specifically, in order to model nonequilibrium stationary states of the system, the external force field is coupled to a dissipative term (thermostat). The well-posedness of the related Cauchy problem is investigated thus allowing the new discrete thermostatted framework to be suitable for the derivation of specific models and the related computational analysis. Applications to crowd dynamics and future research directions are also discussed within the paper.

  2. Planning Sustainable Land-Use: The Experience Of Stella, Missouri

    EPA Science Inventory

    Sustainability has numerous definitions and is often discussed. However, there are fewer lessons derived from its application. The concept for its application evolved out of a study to determine whether brownfields could be redeveloped to be more environmentally friendly. The ...

  3. TECHNICAL APPROACHES TO CHARACTERIZING AND CLEANING UP BROWNFIELDS SITES: RAILROAD YARDS

    EPA Science Inventory

    This guidance document gives assistance to communities, decision-makers, states and municipalities, academia, and the private sector to address issues related to the redevelopment of Brownfields sites, specifically railroad yards. The document helps users to understand the proble...

  4. A GIS connection between brownfield sites, transportation and economic development.

    DOT National Transportation Integrated Search

    2011-08-01

    "This report outlines the design and development of a web-based data distribution system for brownfield site redevelopment in Toledo-Lucas County, Ohio. The system is designed to advance smart growth initiatives by creating the link between transport...

  5. Ready for Reuse in Texas

    EPA Pesticide Factsheets

    EPA has developed a new, long-term corrective action measure of success for all EPA/state cleanup programs that recognizes when a site/facility has been assessed and, if necessary, remediated to the extent that the property is safe for reuse or redevelop

  6. 76 FR 18573 - Notice of Availability of a Final Environmental Impact Statement for the Sunset Area Community...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-04

    ... shopping and commercial space; and green infrastructure. Sunset Terrace's redevelopment provides the... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5513-N-01] Notice of Availability of a.... [[Page 18574

  7. STORM WATER MANAGEMENT MODEL (SWMM) MODERNIZATION

    EPA Science Inventory

    The U.S. Environmental Protection Agency's Water Supply and Water Resources Division in partnership with the consulting firm of CDM to redevelop and modernize the Storm Water Management Model (SWMM). In the initial phase of this project EPA rewrote SWMM's computational engine usi...

  8. BTSC VAPOR INSTRUSION PRIMER "VAPOR INTRUSION CONSIDERATION FOR REDEVELOPMENT"

    EPA Science Inventory

    This primer is designed for brownfields stakeholders concerned about vapor intrusion, including property owners, real estate developers, and contractors performing environmental site investigations. It provides an overview of the vapor intrusion issue and how it can impact the ap...

  9. Anvil Mountain Site, Silverton CO: Technical Memorandum - Energy Efficiency for Affordable Housing at a Brownfield Redevelopment

    EPA Pesticide Factsheets

    This document illustrates how the U.S. Environmental Protection Agency (EPA) Brownfields Program empowers states, communities, and other stakeholders to work together to prevent, assess, safely clean up, and sustainably reuse brownfields.

  10. St. Paul central corridor study : Pierce Butler industrial redevelopment parkway

    DOT National Transportation Integrated Search

    2003-07-01

    At present, development in the St. Paul Central Corridor is occurring piecemeal and lacks an integrative vision. This study's aim was to devise design approaches that create a district which integrates light industrial job creation and retention with...

  11. Stormwater Management for Federal Facilities under Section 438 of the Energy Independence and Security Act

    EPA Pesticide Factsheets

    Federal agencies are required to reduce stormwater runoff from federal development and redevelopment projects to protect water resources. Options include a variety of stormwater management practices like green infrastructure or low impact development

  12. Ready for Reuse in Louisiana

    EPA Pesticide Factsheets

    EPA has developed a new, long-term corrective action measure of success for all EPA/state cleanup programs that recognizes when a site/facility has been assessed and, if necessary, remediated to the extent that the property is safe for reuse or redevelop

  13. Brownfields Allen-Morrison Project: Technical Memorandum on Creating Inventory of Materials that can be Reused or Recycled through Deconstruction

    EPA Pesticide Factsheets

    This technical memorandum presents sustainability pilot activities, a summary of inventory results, and other observations and recommendations for sustainable redevelopment identified during the pilot activities at the brownfields projects.

  14. Fort Ord Redevelopment: Coordinating Transportation and Land Use Planning

    DOT National Transportation Integrated Search

    1999-01-01

    The closure of military bases and conversion to civilian land uses is a challenging task faced by many small communities throughout the nation. Fort Ord is located in Monterey County, California and encompasses 28,000 acres of reprogrammed property. ...

  15. 2016 Revolving Loan Fund Supplemental Awards for Brownfields and Land Revitalization in New England

    EPA Pesticide Factsheets

    EPA has announced grants of nearly $2.6 million in RLF supplemental funding for communities in Connecticut, Maine, Massachusetts, Rhode Island and Vermont to carry out cleanup and redevelopment projects at contaminated Brownfields properties.

  16. 36 CFR 906.1 - Purpose and policy.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... stated in the Congressionally approved Pennsylvania Avenue Plan—1974 is insuring that minority businesses... redevelopment. Accordingly, the Corporation will take affirmative action to assure full minority participation... Affirmative Action Program that affords minorities, women, handicapped persons, and Vietnam era veterans a...

  17. Ready for Reuse in Arkansas

    EPA Pesticide Factsheets

    EPA has developed a new, long-term corrective action measure of success for all EPA/state cleanup programs that recognizes when a site/facility has been assessed and, if necessary, remediated to the extent that the property is safe for reuse or redevelop

  18. Medial gastrocnemius structure and gait kinetics in spastic cerebral palsy and typically developing children: A cross-sectional study.

    PubMed

    Martín Lorenzo, Teresa; Rocon, Eduardo; Martínez Caballero, Ignacio; Lerma Lara, Sergio

    2018-05-01

    To compare medial gastrocnemius muscle-tendon structure, gait propulsive forces, and ankle joint gait kinetics between typically developing children and those with spastic cerebral palsy, and to describe significant associations between structure and function in children with spastic cerebral palsy.A sample of typically developing children (n = 9 /16 limbs) and a sample of children with spastic cerebral palsy (n = 29 /43 limbs) were recruited. Ultrasound and 3-dimensional motion capture were used to assess muscle-tendon structure, and propulsive forces and ankle joint kinetics during gait, respectively.Children with spastic cerebral palsy had shorter fascicles and muscles, and longer Achilles tendons than typically developing children. Furthermore, total negative power and peak negative power at the ankle were greater, while total positive power, peak positive power, net power, total vertical ground reaction force, and peak vertical and anterior ground reaction forces were smaller compared to typically developing children. Correlation analyses revealed that smaller resting ankle joint angles and greater maximum dorsiflexion in children with spastic cerebral palsy accounted for a significant decrease in peak negative power. Furthermore, short fascicles, small fascicle to belly ratios, and large tendon to fascicle ratios accounted for a decrease in propulsive force generation.Alterations observed in the medial gastrocnemius muscle-tendon structure of children with spastic cerebral palsy may impair propulsive mechanisms during gait. Therefore, conventional treatments should be revised on the basis of muscle-tendon adaptations.

  19. The nature of cometary materials

    NASA Technical Reports Server (NTRS)

    Stephens, James

    1989-01-01

    Because cometary surfaces are likely to be far colder and of a different composition than planetary surfaces, there are some new considerations that must be examined in regards to placing instrumented packages or sample return devices on their surfaces. The qualitative analysis of the problem of attaching hardware to a comet and not being ejected back into space can be divided into two parts. The first problem is to pierce the mantle and obtain access to the icy core. Drilling through the mantle requires that the drilling forces be reacted. Reacting such forces probably requires attachment to the icy core below. Therefore, some kinetic impact piercing device is likely to be required as the first act of attachment. The second problem for a piercing device to overcome is the force produced by the impact kinetic energy that tries to eject the piercing device back into space. The mantle and icy core can absorb some of the impact kinetic energy in the form of fracture formation and friction energy. The energy that is not absorbed in these two ways is stored by the core as elastic deformation of the mantle and icy core. It is concluded that because the cometary materials are almost certainly brittle and the icy core is likely to be self lubricating, the elastic rebound and gas pressure expulsion forces must be counteracted by forces greater than those that may be provided by a piercing device or its capture devices (barbs).

  20. Investigation of the heparin-thrombin interaction by dynamic force spectroscopy.

    PubMed

    Wang, Congzhou; Jin, Yingzi; Desai, Umesh R; Yadavalli, Vamsi K

    2015-06-01

    The interaction between heparin and thrombin is a vital step in the blood (anti)coagulation process. Unraveling the molecular basis of the interactions is therefore extremely important in understanding the mechanisms of this complex biological process. In this study, we use a combination of an efficient thiolation chemistry of heparin, a self-assembled monolayer-based single molecule platform, and a dynamic force spectroscopy to provide new insights into the heparin-thrombin interaction from an energy viewpoint at the molecular scale. Well-separated single molecules of heparin covalently attached to mixed self-assembled monolayers are demonstrated, whereby interaction forces with thrombin can be measured via atomic force microscopy-based spectroscopy. Further these interactions are studied at different loading rates and salt concentrations to directly obtain kinetic parameters. An increase in the loading rate shows a higher interaction force between the heparin and thrombin, which can be directly linked to the kinetic dissociation rate constant (koff). The stability of the heparin/thrombin complex decreased with increasing NaCl concentration such that the off-rate was found to be driven primarily by non-ionic forces. These results contribute to understanding the role of specific and nonspecific forces that drive heparin-thrombin interactions under applied force or flow conditions. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Using ground reaction force to predict knee kinetic asymmetry following anterior cruciate ligament reconstruction.

    PubMed

    Dai, B; Butler, R J; Garrett, W E; Queen, R M

    2014-12-01

    Asymmetries in sagittal plane knee kinetics have been identified as a risk factor for anterior cruciate ligament (ACL) re-injury. Clinical tools are needed to identify the asymmetries. This study examined the relationships between knee kinetic asymmetries and ground reaction force (GRF) asymmetries during athletic tasks in adolescent patients following ACL reconstruction (ACL-R). Kinematic and GRF data were collected during a stop-jump task and a side-cutting task for 23 patients. Asymmetry indices between the surgical and non-surgical limbs were calculated for GRF and knee kinetic variables. For the stop-jump task, knee kinetics asymmetry indices were correlated with all GRF asymmetry indices (P < 0.05), except for loading rate. Vertical GRF impulse asymmetry index predicted peak knee moment, average knee moment, and knee work (R(2)  ≥ 0.78, P < 0.01) asymmetry indices. For the side-cutting tasks, knee kinetic asymmetry indices were correlated with the peak propulsion vertical GRF and vertical GRF impulse asymmetry indices (P < 0.05). Vertical GRF impulse asymmetry index predicted peak knee moment, average knee moment, and knee work (R(2)  ≥ 0.55, P < 0.01) asymmetry indices. The vertical GRF asymmetries may be a viable surrogate for knee kinetic asymmetries and therefore may assist in optimizing rehabilitation outcomes and minimizing re-injury rates. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  2. Wellness at Air Command and Staff College: Impacting Total Force Readiness Through Tomorrow’s Leaders

    DTIC Science & Technology

    1998-04-01

    Joseph P. Opatz, ed. [Association for Worksite Health Promotion], Economic Impact of Worksite Health Promotion (Champaign, IL: Human Kinetics Publishers...Employee Health Promotion Programs (Champaign, IL: Human Kinetics Publishers, 1992), viii. 23 Erfurt, et al., 272. 24 O’Donnell and Harris, xiii. 25 Ibid...Champaign, IL: Human Kinetics Publishers, 1992), viii-ix. 40 Glossary ACSC Air Command and Staff College AU Air University AWC Air War College AY Academic

  3. A biomechanical analysis of upper extremity kinetics in children with cerebral palsy using anterior and posterior walkers.

    PubMed

    Konop, Katherine A; Strifling, Kelly M B; Wang, Mei; Cao, Kevin; Schwab, Jeffrey P; Eastwood, Daniel; Jackson, Scott; Ackman, Jeffrey D; Harris, Gerald F

    2009-10-01

    Upper extremity (UE) joint kinetics during aided ambulation is an area of research that is not well characterized in the current literature. Biped UE joints are not anatomically designed to be weight bearing, therefore it is important to quantify UE kinetics during assisted gait. This will help to better understand the biomechanical implications of UE weight bearing, and enable physicians to prescribe more effective methods for treatment and therapy, perhaps minimizing excessive loads and torques. To address this challenge, an UE model that incorporates both kinematics and kinetics has been developed for use with walkers instrumented with load cells. In this study, the UE joint kinetics are calculated for 10 children with cerebral palsy using both anterior and posterior walkers. Three-dimensional joint reaction forces and moments are fully characterized for the wrist, elbow, and shoulder (glenohumeral) joints for both walker types. Statistical analysis methods are used to quantify the differences in forces or moments between the two walker types. Comparisons showed no significant differences in kinetic joint parameters between walker types. Results from a power analysis of the current data are provided which may be useful for planning longer term clinical studies. If risk factors for UE joint pathology can be identified early, perhaps a change in gait training routine, walker prescription, or walker design could prevent further harm.

  4. Analysis of a kinetic multi-segment foot model part II: kinetics and clinical implications.

    PubMed

    Bruening, Dustin A; Cooney, Kevin M; Buczek, Frank L

    2012-04-01

    Kinematic multi-segment foot models have seen increased use in clinical and research settings, but the addition of kinetics has been limited and hampered by measurement limitations and modeling assumptions. In this second of two companion papers, we complete the presentation and analysis of a three segment kinetic foot model by incorporating kinetic parameters and calculating joint moments and powers. The model was tested on 17 pediatric subjects (ages 7-18 years) during normal gait. Ground reaction forces were measured using two adjacent force platforms, requiring targeted walking and the creation of two sub-models to analyze ankle, midtarsal, and 1st metatarsophalangeal joints. Targeted walking resulted in only minimal kinematic and kinetic differences compared with walking at self selected speeds. Joint moments and powers were calculated and ensemble averages are presented as a normative database for comparison purposes. Ankle joint powers are shown to be overestimated when using a traditional single-segment foot model, as substantial angular velocities are attributed to the mid-tarsal joint. Power transfer is apparent between the 1st metatarsophalangeal and mid-tarsal joints in terminal stance/pre-swing. While the measurement approach presented here is limited to clinical populations with only minimal impairments, some elements of the model can also be incorporated into routine clinical gait analysis. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. Identifying the role of historical anthropogenic activities on urban soils: geochemical impact and city scale mapping

    NASA Astrophysics Data System (ADS)

    Le Guern, Cecile; Baudouin, Vivien; Conil, Pierre

    2017-04-01

    Recently, European cities have faced several changes including deindustrialization and population increase. To limit urban sprawl, urban densification is preferred. It conducts to (re)develop available areas such as brownfields. Although these areas can be attractive for housing due to their location (in proximity to the city centre or to a riverside), their soils and subsoils are often contaminated. They are therefore potentially harmful for human health and the environment, and potentially costly to remediate. Currently, in case of contamination suspicion, depth geochemical characterization of urban soil and subsoil are carried out at site scale. Nevertheless, large redevelopment project occur at quarter to city scale. It appears therefore useful to acquire the preliminary knowledge on the structure and quality of soil and subsoils, as well as on the potential sources of contamination at quarter to city scale. In the frame of the Ile de Nantes (France) redevelopment project, we considered more particularly anthropogenic deposits and former industrial activities as main sources of contamination linked to human activities. To face the low traceability of the use of anthropogenic deposits and the lack of synthesis of former industrial activities, we carried out a historical study, synthetizing the information spread in numerous archive documents to spatialize the extent of the deposits and of the former activities. In addition we developed a typology of made grounds according to their contamination potential to build a 3D geological model with a geochemical coherence. In this frame, we valorized existing borehole descriptions coming mainly from pollution diagnosis and geotechnical studies. We also developed a methodology to define urban baseline compatibility levels using the existing analytical data at depth from pollution diagnosis. These data were previously gathered in a local geodatabase towards with borehole descriptions (more than 2000 borehole descriptions, more than 1800 analyzed samples, almost 100 000 analyzed parameters). The potential quality of soil and subsoil was spatialized in 2D and 3D on the basis of anthropogenic deposits structure and typology as well as of the potential sources of contamination linked to former industrial activities. Volumes were also calculated to help the developer anticipating the management of excavated materials. Comparison with effective soil and subsoil quality (existing chemical data) shows fairly good anticipation of contamination problems, confirming the interest of spatializing the historical anthropogenic activities to anticipate the quality of urban soil and subsoil and guide city scale mapping. Urban geochemical compatibility levels will be used operationally to enhance the reuse of excavated materials. A better knowledge of soils and subsoils at depth is very useful to optimize urban redevelopment projects, anticipating contamination problems, and managing excavated materials (e.g. local reuse possibilities, disposal costs etc.). The potential economic, environmental and social consequences render it essential for urban sustainable development. 3D geochemical characterization of soil and subsoil for urban (re)development is an ambitious task. Rarely carried out until now, it needs improved development of acquisition, management, visualisation and use of data.

  6. The effect that state and federal housing policies have on vehicle miles of travel.

    DOT National Transportation Integrated Search

    2016-11-07

    This report examines the ability of existing and proposed affordable housing policies to align : with sustainable transportation goals in California. First, we compare the ability of Low Income : Housing Tax Credit (LIHTC) and Redevelopment funded pr...

  7. SMARTE: IMPROVING REVITALIZATION DECISIONS (BERLIN, GERMANY)

    EPA Science Inventory

    The U.S.-German Bilateral Working Group is developing Site-specific Management Approaches and Redevelopment Tools (SMART). In the U.S., the SMART compilation is housed in a web-based, decision support tool called SMARTe. All tools within SMARTe that are developed specifically for...

  8. VERIFYING THE SOCIAL, ENVIRONMENTAL, AND ECONOMIC PROMISE OF BROWNFIELD PROGRAMS

    EPA Science Inventory

    The purpose of this research is to develop a nationally replicable methodology to empirically assess the social, economic, and environmental benefits of brownfields redevelopment for low-to-moderate income communities. Using the City of Charlottes (NC) brownfields program, we i...

  9. COST ESTIMATING TOOLS AND RESOURCES FOR ADDRESSING SITES UNDER THE BROWNFIELDS INITIATIVE

    EPA Science Inventory

    Brownfields redevelopment contributes to the revitalization of communities across the U.S. Reuse of these abandoned, contaminated sites spurs economic growth, builds community pride, protects public health, and helps maintain our nation's "greenfields," often at a relatively low ...

  10. SITE-SPECIFIC MANAGEMENT APPROACHES AND REDEVELOPMENT TOOLS - TECHNOLOGIES (SMARTTECH)

    EPA Science Inventory

    The United States Environmental Protection Agency (EPA) and the German Federal Ministry of Education and Research (BMBF) continues an ongoing partnership to gain an understanding of each other's approach to the cleanup of chemical contamination in order to protect human health an...

  11. 32 CFR 174.4 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... property. Federal law provides the Department with an array of legal authorities, including public benefit... government, conservation conveyances, and public sales, by which to transfer property on closed or realigned... transfer property in a timely manner and provide a foundation for solid economic redevelopment. (e) Speak...

  12. 32 CFR 174.4 - Policy.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... property. Federal law provides the Department with an array of legal authorities, including public benefit... government, conservation conveyances, and public sales, by which to transfer property on closed or realigned... transfer property in a timely manner and provide a foundation for solid economic redevelopment. (e) Speak...

  13. 32 CFR 174.4 - Policy.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... property. Federal law provides the Department with an array of legal authorities, including public benefit... government, conservation conveyances, and public sales, by which to transfer property on closed or realigned... transfer property in a timely manner and provide a foundation for solid economic redevelopment. (e) Speak...

  14. Integrating Green Infrastructure with Community Needs: El Cerrito Green Streets Project

    EPA Pesticide Factsheets

    The El Cerrito Redevelopment Agency, in combination with the San Francisco Estuary Partnership, obtained a $392,000 loan with 100 principal forgiveness through ARRA for the construction of a series of rain gardens and bioswales along San Pablo Avenue.

  15. Sustainable Urban Waters: Opportunities to Integrate Environmental Protection in Multi-objective Projects

    EPA Science Inventory

    Abstract: Nonpoint source pollution is an ongoing challenge for environmental agencies who seek to protect waters of the U.S. Urban stream and waterfront redevelopment projects present opportunities to achieve integrated environmental, economic, and social benefits in urban water...

  16. Residential Construction Trends in America's Metropolitan Regions: 2010 and 2009 Editions

    EPA Pesticide Factsheets

    These two reports examine residential building permits in the 50 largest U.S. metropolitan regions at the county or jurisdictional level to determine whether there has been a shift toward redevelopment and which regions have seen the most change.

  17. 32 CFR 174.4 - Policy.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... property. Federal law provides the Department with an array of legal authorities, including public benefit... government, conservation conveyances, and public sales, by which to transfer property on closed or realigned... transfer property in a timely manner and provide a foundation for solid economic redevelopment. (e) Speak...

  18. 32 CFR 174.4 - Policy.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... property. Federal law provides the Department with an array of legal authorities, including public benefit... government, conservation conveyances, and public sales, by which to transfer property on closed or realigned... transfer property in a timely manner and provide a foundation for solid economic redevelopment. (e) Speak...

  19. EPA and partners celebrate redevelopment at Charles George Landfill Superfund Site

    EPA Pesticide Factsheets

    The Charles George Reclamation Trust Landfill Superfund site, a former landfill, is now home to a new solar facility. The USEPA oversaw the cleanup of the 70-acre Superfund site, preventing any exposure to contaminants and reducing leachate generation.

  20. EPA Science Matters Newsletter: Greener Cleanups at Hazardous Waste Sites (Published August 2013)

    EPA Pesticide Factsheets

    Read about the EPA’s Smart Energy Resources Guide (SERG). The guide covers techniques for superfund managers to reduce cleanup emissions in a process called green remediation, and can be used by any site remediation and redevelopment manager.

  1. SITE-SPECIFIC MANAGEMENT APPROACHES AND REDEVELOPMENT TOOLS (SMART) - presentation 04

    EPA Science Inventory

    The United States Environmental Protection Agency (EPA) and the German Federal Ministry of Education and Research (BMBF) created a Bilateral Working Group in 1990 to gain a better understanding of each country's efforts in developing and demonstrating remedial technologies (Phase...

  2. Concurrent validity and reliability of using ground reaction force and center of pressure parameters in the determination of leg movement initiation during single leg lift.

    PubMed

    Aldabe, Daniela; de Castro, Marcelo Peduzzi; Milosavljevic, Stephan; Bussey, Melanie Dawn

    2016-09-01

    Postural adjustment evaluations during single leg lift requires the initiation of heel lift (T1) identification. T1 measured by means of motion analyses system is the most reliable approach. However, this method involves considerable workspace, expensive cameras, and time processing data and setting up laboratory. The use of ground reaction forces (GRF) and centre of pressure (COP) data is an alternative method as its data processing and setting up is less time consuming. Further, kinetic data is normally collected using frequency samples higher than 1000Hz whereas kinematic data are commonly captured using 50-200Hz. This study describes the concurrent-validity and reliability of GRF and COP measurements in determining T1, using a motion analysis system as reference standard. Kinematic and kinetic data during single leg lift were collected from ten participants. GRF and COP data were collected using one and two force plates. Displacement of a single heel marker was captured by means of ten Vicon(©) cameras. Kinetic and kinematic data were collected using a sample frequency of 1000Hz. Data were analysed in two stages: identification of key events in the kinetic data, and assessing concurrent validity of T1 based on the chosen key events with T1 provided by the kinematic data. The key event presenting the least systematic bias, along with a narrow 95% CI and limits of agreement against the reference standard T1, was the Baseline COPy event. Baseline COPy event was obtained using one force plate and presented excellent between-tester reliability. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. The Kinematics and Kinetics of the Running Pattern of Two-, Four-, and Six-Year-Old Children.

    ERIC Educational Resources Information Center

    Fortney, Virginia L.

    1983-01-01

    The running patterns of two-, four-, and six-year-old children were analyzed to determine how age and sex differences affected selected kinematic and kinetic variables. Differences tended to involve displacement, velocity, and magnitude of force measures. Sex differences concerning the leg swing were noted. (Author/PP)

  4. Downhill Cycling Symmetry Breaking: How the Rider Foils Experiment

    ERIC Educational Resources Information Center

    Abu, Yuval Ben; Wolfson, Ira; Bran, Gil; Yizhaq, Hezi

    2017-01-01

    In high-school teaching of mechanics, we deal, among other things, with the nature of static and kinetic friction, forces that are proportional to the normal force. Under the influence of frictional forces, a body moves down a rough sloped decline at a fixed rate of acceleration that is independent of its mass. This situation does not apply to…

  5. Probing effects of pH change on dynamic response of Claudin-2 mediated adhesion using single molecule force spectroscopy.

    PubMed

    Lim, Tong Seng; Vedula, Sri Ram Krishna; Hui, Shi; Kausalya, P Jaya; Hunziker, Walter; Lim, Chwee Teck

    2008-08-15

    Claudins belong to a large family of transmembrane proteins that localize at tight junctions (TJs) where they play a central role in regulating paracellular transport of solutes and nutrients across epithelial monolayers. Their ability to regulate the paracellular pathway is highly influenced by changes in extracellular pH. However, the effect of changes in pH on the strength and kinetics of claudin mediated adhesion is poorly understood. Using atomic force microscopy, we characterized the kinetic properties of homophilic trans-interactions between full length recombinant GST tagged Claudin-2 (Cldn2) under different pH conditions. In measurements covering three orders of magnitude change in force loading rate of 10(2)-10(4) pN/s, the Cldn2/Cldn2 force spectrum (i.e., unbinding force versus loading rate) revealed a fast and a slow loading regime that characterized a steep inner activation barrier and a wide outer activation barrier throughout pH range of 4.5-8. Comparing to the neutral condition (pH 6.9), differences in the inner energy barriers for the dissociation of Cldn2/Cldn2 mediated interactions at acidic and alkaline environments were found to be <0.65 k(B)T, which is much lower than the outer dissociation energy barrier (>1.37 k(B)T). The relatively stable interaction of Cldn2/Cldn2 in neutral environment suggests that electrostatic interactions may contribute to the overall adhesion strength of Cldn2 interactions. Our results provide an insight into the changes in the inter-molecular forces and adhesion kinetics of Cldn2 mediated interactions in acidic, neutral and alkaline environments.

  6. Regulation of Catch Bonds by Rate of Force Application*

    PubMed Central

    Sarangapani, Krishna K.; Qian, Jin; Chen, Wei; Zarnitsyna, Veronika I.; Mehta, Padmaja; Yago, Tadayuki; McEver, Rodger P.; Zhu, Cheng

    2011-01-01

    The current paradigm for receptor-ligand dissociation kinetics assumes off-rates as functions of instantaneous force without impact from its prior history. This a priori assumption is the foundation for predicting dissociation from a given initial state using kinetic equations. Here we have invalidated this assumption by demonstrating the impact of force history with single-bond kinetic experiments involving selectins and their ligands that mediate leukocyte tethering and rolling on vascular surfaces during inflammation. Dissociation of bonds between L-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) loaded at a constant ramp rate to a constant hold force behaved as catch-slip bonds at low ramp rates that transformed to slip-only bonds at high ramp rates. Strikingly, bonds between L-selectin and 6-sulfo-sialyl Lewis X were impervious to ramp rate changes. This ligand-specific force history effect resembled the effect of a point mutation at the L-selectin surface (L-selectinA108H) predicted to contact the former but not the latter ligand, suggesting that the high ramp rate induced similar structural changes as the mutation. Although the A108H substitution in L-selectin eliminated the ramp rate responsiveness of its dissociation from PSGL-1, the inverse mutation H108A in P-selectin acquired the ramp rate responsiveness. Our data are well explained by the sliding-rebinding model for catch-slip bonds extended to incorporate the additional force history dependence, with Ala-108 playing a pivotal role in this structural mechanism. These results call for a paradigm shift in modeling the mechanical regulation of receptor-ligand bond dissociation, which includes conformational coupling between binding pocket and remote regions of the interacting molecules. PMID:21775439

  7. Career redevelopment programmes for inactive nurses in Japan.

    PubMed

    Tanaka, Sachiko; Serizawa, Takako; Sakaguchi, Chizuru

    2008-12-01

    The purpose of this paper is to examine the challenges and problems in using career redevelopment programmes and individual hospital programmes to prepare inactive nurses to re-enter into the workforce in Japan. It is critical to supply sufficient skilled health human resources for medical care. Although, Japan has a mandatory retraining programme for supporting nurses to return to the workplace after a career break, it is unclear to what extent there are benefits to nurses from these programmes. The research of career redevelopment programme was undertaken in three administrative divisions' nurse centres in local prefecture A, B and C. A survey of nurses participating in the programme running in T Hospital was also conducted. The issues examined were the background and motivations of participants, the length of career break, the percentages returning to work and the effectiveness of each programme. The average age of participants was 40 years, ranging widely from the 20-60 years. Local prefecture A tended to have narrower age range than others, namely from the 30-50 years. The average period of career break was around eight years at two of three. Length of experience was quite varied from entry level to 20 or 30 years in nursing. Feedback from nurses in the case study T Hospital suggests that the most effective ways of providing support through the programme was to meet the need for continuing support, including working styles after return to work and using the resources programme in their own area of domicile. In the potential return of the nurse, the following are important: (i) job support system by using social resources effectively in the community level; and (ii) introduction of diverse working styles that take account of varying work-life balance, as well as childcare support, by using existing facilities or human resources.

  8. A Brownfields strategy for the Toronto Port Area

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ibbotson, B.G.; Benson, B.A.

    The Port Area of Toronto consists of several hundred acres, much of it created by filling near-shore sections of the Inner Harbour. The quality of the fill materials and the industrial activities that have taken place in the Port Area have resulted in soil and/or ground water quality conditions at many locations that do not meet current regulatory criteria and guidelines. As the administrator of properties representing more than 400 acres in the Port Area, the City of Toronto Economic Development Corporation (TEDCO) has a leadership role in the redevelopment of the Port Area. To date, a few TEDCO sitesmore » have always been restored and redeveloped on an individual basis, with little attention paid to ground water issues. To move forward on other redevelopment initiatives and to attract capital, it is necessary to increase the certainty with respect to regulatory requirements, the distribution of liabilities, and those parts of decision-making processes that consider soil and ground water issues. To address these needs, TEDCO has designed an overall soil and ground water management strategy that can be applied to its properties in the Port Area. The resulting strategy consists of four interrelated parts: an area-wide initiative to monitor ground water characteristics and assess ecological conditions; a collection of three protocols for managing individual sites; an information management system; and direction on the administration of the strategy. Together, the four parts provide a comprehensive and pragmatic approach to managing soil and ground water on TEDCO properties. The use of a multi-party agreement to formalize the strategy and specify roles and responsibilities of TEDCO, the municipality, and the Ontario Ministry of Environment and Energy is recommended.« less

  9. Using a Health in All Policies Approach to Address Social Determinants of Sexually Transmitted Disease Inequities in the Context of Community Change and Redevelopment

    PubMed Central

    Fuller, Elizabeth; Branscomb, Jane; Cheung, Karen; Reed, Phillip Jackson; Wong, Naima; Henderson, Michael; Williams, Samantha

    2013-01-01

    Objectives We used a Health in All Policies (HiAP) framework to determine what data, policy, and community efficacy opportunities exist for improving sexual health and reducing sexually transmitted diseases (STDs) in an area surrounding an Army base undergoing redevelopment in Atlanta, Georgia. Methods We conducted a literature review, consulted with experts, mapped social determinants in the community, conducted key informant interviews with community leaders to explore policy solutions, used Photovoice with community members to identify neighborhood assets, and shared data with all stakeholder groups to solicit engagement for next steps. Results We identified the following HiAP-relevant determinants of STD inequities in the literature: education, employment, male incarceration, drug and alcohol marketing, and social capital. Quantitative data confirmed challenges in education, employment, and male incarceration in the area. Interviews identified policy opportunities such as educational funding ratios, Community Hire Agreements, code and law enforcement, addiction and mental health resources, lighting for safety, and a nonemergency public safety number. Photovoice participants identified community assets to protect including family-owned businesses, green spaces, gathering places, public transportation resources, historical sites, and architectural elements. Stakeholder feedback provided numerous opportunities for next steps. Conclusions This study contributes to the HiAP literature by providing an innovative mixed-methods design that locates social determinants of STDs within a geographic context, identifies policy solutions from local leaders, highlights community assets through the lens of place attachment, and engages stakeholders in identifying next steps. Findings from this study could inform other redevelopments, community-based studies of STDs, and HiAP efforts. PMID:24179283

  10. Glass microneedles for force measurements: a finite-element analysis model

    PubMed Central

    Ayittey, Peter N.; Walker, John S.; Rice, Jeremy J.; de Tombe, Pieter P.

    2010-01-01

    Changes in developed force (0.1–3.0 μN) observed during contraction of single myofibrils in response to rapidly changing calcium concentrations can be measured using glass microneedles. These microneedles are calibrated for stiffness and deflect on response to developed myofibril force. The precision and accuracy of kinetic measurements are highly dependent on the structural and mechanical characteristics of the microneedles, which are generally assumed to have a linear force–deflection relationship. We present a finite-element analysis (FEA) model used to simulate the effects of measurable geometry on stiffness as a function of applied force and validate our model with actual measured needle properties. In addition, we developed a simple heuristic constitutive equation that best describes the stiffness of our range of microneedles used and define limits of geometry parameters within which our predictions hold true. Our model also maps a relation between the geometry parameters and natural frequencies in air, enabling optimum parametric combinations for microneedle fabrication that would reflect more reliable force measurement in fluids and physiological environments. We propose a use for this model to aid in the design of microneedles to improve calibration time, reproducibility, and precision for measuring myofibrillar, cellular, and supramolecular kinetic forces. PMID:19104827

  11. Role of small oligomers on the amyloidogenic aggregation free-energy landscape.

    PubMed

    He, Xianglan; Giurleo, Jason T; Talaga, David S

    2010-01-08

    We combine atomic-force-microscopy particle-size-distribution measurements with earlier measurements on 1-anilino-8-naphthalene sulfonate, thioflavin T, and dynamic light scattering to develop a quantitative kinetic model for the aggregation of beta-lactoglobulin into amyloid. We directly compare our simulations to the population distributions provided by dynamic light scattering and atomic force microscopy. We combine species in the simulation according to structural type for comparison with fluorescence fingerprint results. The kinetic model of amyloidogenesis leads to an aggregation free-energy landscape. We define the roles of and propose a classification scheme for different oligomeric species based on their location in the aggregation free-energy landscape. We relate the different types of oligomers to the amyloid cascade hypothesis and the toxic oligomer hypothesis for amyloid-related diseases. We discuss existing kinetic mechanisms in terms of the different types of oligomers. We provide a possible resolution to the toxic oligomer-amyloid coincidence.

  12. A thermostatted kinetic theory model for event-driven pedestrian dynamics

    NASA Astrophysics Data System (ADS)

    Bianca, Carlo; Mogno, Caterina

    2018-06-01

    This paper is devoted to the modeling of the pedestrian dynamics by means of the thermostatted kinetic theory. Specifically the microscopic interactions among pedestrians and an external force field are modeled for simulating the evacuation of pedestrians from a metro station. The fundamentals of the stochastic game theory and the thermostatted kinetic theory are coupled for the derivation of a specific mathematical model which depicts the time evolution of the distribution of pedestrians at different exits of a metro station. The perturbation theory is employed in order to establish the stability analysis of the nonequilibrium stationary states in the case of a metro station consisting of two exits. A general sensitivity analysis on the initial conditions, the magnitude of the external force field and the number of exits is presented by means of numerical simulations which, in particular, show how the asymptotic distribution and the convergence time are affected by the presence of an external force field. The results show how, in evacuation conditions, the interaction dynamics among pedestrians can be negligible with respect to the external force. The important role of the thermostat term in allowing the reaching of the nonequilibrium stationary state is stressed out. Research perspectives are underlined at the end of paper, in particular for what concerns the derivation of frameworks that take into account the definition of local external actions and the introduction of the space and velocity dynamics.

  13. Kinematics and Kinetics of Maximum Running Speed in Youth Across Maturity.

    PubMed

    Rumpf, Michael C; Cronin, John B; Oliver, Jonathan; Hughes, Michael

    2015-05-01

    Sprinting is an important physical capacity and the development of sprint ability can take place throughout the athlete's growth. The purpose of this study therefore was to determine if the kinematics and kinetics associated with maximum sprint velocity differs in male youth participants of different maturity status (pre, mid- and postpeak height velocity (PHV)) and if maximum sprint velocity is determined by age, maturity or individual body size measurement. Participants (n = 74) sprinted over 30 meters on a nonmotorized treadmill and the fastest four consecutive steps were analyzed. Pre-PHV participants were found to differ significantly (p < .05) to mid- and post-PHV participants in speed, step length, step frequency, vertical and horizontal force, and horizontal power (~8-78%). However, only relative vertical force and speed differed significantly between mid and post-PHV groups. The greatest average percent change in kinetics and kinematics was observed from pre- to mid-PHV (37.8%) compared with mid- to post- PHV groups (11.6%). When maturity offset was entered as a covariate, there was no significant difference in velocity between the three groups. However, all groups were significantly different from each other when age was chosen as the covariate. The two best predictors of maximal velocity within each maturity group were power and horizontal force (R2 = 97-99%) indicating the importance of horizontal force application while sprinting. Finally, maturity explained 83% of maximal velocity across all groups.

  14. Interactions between vortex tubes and magnetic-flux rings at high kinetic and magnetic Reynolds numbers

    NASA Astrophysics Data System (ADS)

    Kivotides, Demosthenes

    2018-03-01

    The interactions between vortex tubes and magnetic-flux rings in incompressible magnetohydrodynamics are investigated at high kinetic and magnetic Reynolds numbers, and over a wide range of the interaction parameter. The latter is a measure of the turnover time of the large-scale fluid motions in units of the magnetic damping time, or of the strength of the Lorentz force in units of the inertial force. The small interaction parameter results, which are related to kinematic turbulent dynamo studies, indicate the evolution of magnetic rings into flattened spirals wrapped around the vortex tubes. This process is also observed at intermediate interaction parameter values, only now the Lorentz force creates new vortical structures at the magnetic spiral edges, which have a striking solenoid vortex-line structure, and endow the flattened magnetic-spiral surfaces with a curvature. At high interaction parameter values, the decisive physical factor is Lorentz force effects. The latter create two (adjacent to the magnetic ring) vortex rings that reconnect with the vortex tube by forming an intriguing, serpentinelike, vortex-line structure, and generate, in turn, two new magnetic rings, adjacent to the initial one. In this regime, the morphologies of the vorticity and magnetic field structures are similar. The effects of these structures on kinetic and magnetic energy spectra, as well as on the direction of energy transfer between flow and magnetic fields, are also indicated.

  15. Kinetic Assessment of Golf Shoe Outer Sole Design Features

    PubMed Central

    Smith, Neal A.; Dyson, Rosemary J.

    2009-01-01

    This study assessed human kinetics in relation to golf shoe outer sole design features during the golf swing using a driver club by measuring both within the shoe, and beneath the shoe at the natural grass interface. Three different shoes were assessed: metal 7- spike shoe, alternative 7-spike shoe, and a flat soled shoe. In-shoe plantar pressure data were recorded using Footscan RS International pressure insoles and sampling at 500 Hz. Simultaneously ground reaction force at the shoe outer sole was measured using 2 natural grass covered Kistler force platforms and 1000 Hz data acquisition. Video recording of the 18 right-handed golfers at 200 Hz was undertaken while the golfer performed 5 golf shots with his own driver in each type of shoe. Front foot (nearest to shot direction) maximum vertical force and torque were greater than at the back foot, and there was no significant difference related to the shoe type. Wearing the metal spike shoe when using a driver was associated with more torque generation at the back foot (p < 0. 05) than when the flat soled shoe was worn. Within shoe regional pressures differed significantly with golf shoe outer sole design features (p < 0.05). Comparison of the metal spike and alternative spike shoe results provided indications of the quality of regional traction on the outer sole. Potential golf shoe outer sole design features and traction were presented in relation to phases of the golf swing movement. Application of two kinetic measurement methods identified that moderated (adapted) muscular control of foot and body movement may be induced by golf shoe outer sole design features. Ground reaction force measures inform comparisons of overall shoe functional performance, and insole pressure measurements inform comparisons of the underfoot conditions induced by specific regions of the golf shoe outer sole. Key points Assessments of within golf shoe pressures and beneath shoe forces at the natural grass interface were conducted during golf shots with a driver. Application of two kinetic measurement methods simultaneously identified that moderated (adapted) muscular control of the foot and body movement may be induced by golf shoe outer sole localised design features. Ground force measures inform overall shoe kinetic functional performance. Insole pressure measurement informs of underfoot conditions induced by localised specific regions of the golf outer sole. Significant differences in ground-shoe torque generation and insole regional pressures were identified when different golf shoes were worn. PMID:24149603

  16. Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.

    PubMed

    Wu, Xin-Sheng; Lee, Sung Hoon; Sheng, Jiansong; Zhang, Zhen; Zhao, Wei-Dong; Wang, Dongsheng; Jin, Yinghui; Charnay, Patrick; Ervasti, James M; Wu, Ling-Gang

    2016-12-07

    Mechanical force is needed to mediate endocytosis. Whether actin, the most abundant force-generating molecule, is essential for endocytosis is highly controversial in mammalian cells, particularly synapses, likely due to the use of actin blockers, the efficiency and specificity of which are often unclear in the studied cell. Here we addressed this issue using a knockout approach combined with measurements of membrane capacitance and fission pore conductance, imaging of vesicular protein endocytosis, and electron microscopy. We found that two actin isoforms, β- and γ-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. Polymerized actin provides mechanical force to form endocytic pits. Actin also facilitates replenishment of the readily releasable vesicle pool, likely via endocytic clearance of active zones. We conclude that polymerized actin provides mechanical force essential for all kinetically distinguishable forms of endocytosis at synapses. Published by Elsevier Inc.

  17. Actin is crucial for all kinetically distinguishable forms of endocytosis at synapses

    PubMed Central

    Wu, Xin-Sheng; Lee, Sunghoon; Sheng, Jiansong; Zhang, Zhen; Zhao, Weidong; Wang, Dongsheng; Jin, Yinghui; Charnay, Patrick; Ervasti, James M.; Wu, Ling-Gang

    2016-01-01

    Summary Mechanical force is needed to mediate endocytosis. Whether actin, the most abundant force-generating molecule, is essential for endocytosis is highly controversial in mammalian cells, particularly synapses, likely due to the use of actin blockers, the efficiency and specificity of which are often unclear in the studied cell. Here we addressed this issue using knockout approach combined with measurements of membrane capacitance and fission pore conductance, imaging of vesicular protein endocytosis, and electron microscopy. We found that two actin isoforms, β- and γ-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. Polymerized actin provides mechanical force to form endocytic pits. Actin also facilitates replenishment of the readily releasable vesicle pool, likely via endocytic clearance of active zones. We conclude that polymerized actin provides mechanical force essential for all kinetically distinguishable forms of endocytosis at synapses. PMID:27840001

  18. Tuning the driving force for exciton dissociation in single-walled carbon nanotube heterojunctions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ihly, Rachelle; Mistry, Kevin S.; Ferguson, Andrew J.

    2016-04-25

    Understanding the kinetics and energetics of interfacial electron transfer in molecular systems is crucial for the development of a broad array of technologies, including photovoltaics, solar fuel systems and energy storage. The Marcus formulation for electron transfer relates the thermodynamic driving force and reorganization energy for charge transfer between a given donor/acceptor pair to the kinetics and yield of electron transfer. Here we investigated the influence of the thermodynamic driving force for photoinduced electron transfer (PET) between single-walled carbon nanotubes (SWCNTs) and fullerene derivatives by employing time-resolved microwave conductivity as a sensitive probe of interfacial exciton dissociation. For the firstmore » time, we observed the Marcus inverted region (in which driving force exceeds reorganization energy) and quantified the reorganization energy for PET for a model SWCNT/acceptor system. The small reorganization energies (about 130 meV, most of which probably arises from the fullerene acceptors) are beneficial in minimizing energy loss in photoconversion schemes.« less

  19. Factors that influence ground reaction force profiles during counter movement jumping.

    PubMed

    Eagles, Alexander N; Sayers, Mark G; Lovell, Dale I

    2017-05-01

    The purpose of this study was to examine how hip, knee and ankle kinetics and kinematics influence effective impulse production during countermovement jumps. Eighteen semi-professional soccer players (22.8±2.2 years) volunteered to participate in the study. Participants completed three maximal countermovement jumps on two force platforms (1000 Hz) that were linked to a nine camera infrared motion capture system (500 Hz). Kinetic and kinematic data revealed jumpers who fail to achieve uniform ground reaction force curves that result in optimal impulse production during their jump always display hip adduction and or hip internal rotation during the concentric phase of the countermovement jump. The variation of hip adduction and or internal rotation likely represents failed joint transition during the concentric phase of the countermovement jump and appears to account for a non-uniform force trace seen in these jumpers. The findings suggest rehabilitation and conditioning exercises for injury prevention and performance may benefit from targeting frontal and transverse plane movement.

  20. Investigating the Mechanical Properties of Plasma von Willebrand Factor Using Atomic Force Microscopy

    NASA Astrophysics Data System (ADS)

    Wijeratne, Sitara; Botello, Eric; Yeh, Hui-Chun; Zhou, Zhou; Bergeron, Angela; Frey, Eric; Moake, Joel; Dong, Jing-Fei; Kiang, Ching-Hwa

    2011-10-01

    Single-molecule manipulation allows us to study the real-time kinetics of complex cellular processes. The mechanochemistry of different forms of von Willebrand factor (VWF) and their receptor-ligand binding kinetics can be probed by atomic force microscopy (AFM). Since plasma VWF can be activated upon shear, the structural and functional properties of VWF that are critical in mediating thrombus formation become important. Here we characterized the mechanical resistance to domain unfolding of VWF to determine its conformational states. We found the shear-induced conformational changes, hence the mechanical property, can be detected by the change in unfolding forces. The relaxation rate of such effect is much longer than expected. Our results offer an insight in establishing strategies for regulating VWF adhesion activity, increasing our understanding of surface-induced thrombosis as mediated by VWF.

  1. Wheelchair pushrim kinetics measurement: A method to cancel inaccuracies due to pushrim weight and wheel camber.

    PubMed

    Chénier, Félix; Aissaoui, Rachid; Gauthier, Cindy; Gagnon, Dany H

    2017-02-01

    The commercially available SmartWheel TM is largely used in research and increasingly used in clinical practice to measure the forces and moments applied on the wheelchair pushrims by the user. However, in some situations (i.e. cambered wheels or increased pushrim weight), the recorded kinetics may include dynamic offsets that affect the accuracy of the measurements. In this work, an automatic method to identify and cancel these offsets is proposed and tested. First, the method was tested on an experimental bench with different cambers and pushrim weights. Then, the method was generalized to wheelchair propulsion. Nine experienced wheelchair users propelled their own wheelchairs instrumented with two SmartWheels with anti-slip pushrim covers. The dynamic offsets were correctly identified using the propulsion acquisition, without needing a separate baseline acquisition. A kinetic analysis was performed with and without dynamic offset cancellation using the proposed method. The most altered kinetic variables during propulsion were the vertical and total forces, with errors of up to 9N (p<0.001, large effect size of 5). This method is simple to implement, fully automatic and requires no further acquisitions. Therefore, we advise to use it systematically to enhance the accuracy of existing and future kinetic measurements. Copyright © 2016 IPEM. Published by Elsevier Ltd. All rights reserved.

  2. A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation

    NASA Astrophysics Data System (ADS)

    Limozin, Laurent; Bongrand, Pierre; Robert, Philippe

    2016-10-01

    Antibodies and B cell receptors often bind their antigen at cell-cell interface while both molecular species are surface-bound, which impacts bond kinetics and function. Despite the description of complex energy landscapes for dissociation kinetics which may also result in significantly different association kinetics, surface-bound molecule (2D) association kinetics usually remain described by an on-rate due to crossing of a single free energy barrier, and few experimental works have measured association kinetics under conditions implying force and two-dimensional relative ligand-receptor motion. We use a new laminar flow chamber to measure 2D bond formation with systematic variation of the distribution of encounter durations between antigen and antibody, in a range from 0.1 to 10 ms. Under physiologically relevant forces, 2D association is 100-fold slower than 3D association as studied by surface plasmon resonance assays. Supported by brownian dynamics simulations, our results show that a minimal encounter duration is required for 2D association; an energy landscape featuring a rough initial part might be a reasonable way of accounting for this. By systematically varying the temperature of our experiments, we evaluate roughness at 2kBT, in the range of previously proposed rough parts of landscapes models during dissociation.

  3. A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation

    PubMed Central

    Limozin, Laurent; Bongrand, Pierre; Robert, Philippe

    2016-01-01

    Antibodies and B cell receptors often bind their antigen at cell-cell interface while both molecular species are surface-bound, which impacts bond kinetics and function. Despite the description of complex energy landscapes for dissociation kinetics which may also result in significantly different association kinetics, surface-bound molecule (2D) association kinetics usually remain described by an on-rate due to crossing of a single free energy barrier, and few experimental works have measured association kinetics under conditions implying force and two-dimensional relative ligand-receptor motion. We use a new laminar flow chamber to measure 2D bond formation with systematic variation of the distribution of encounter durations between antigen and antibody, in a range from 0.1 to 10 ms. Under physiologically relevant forces, 2D association is 100-fold slower than 3D association as studied by surface plasmon resonance assays. Supported by brownian dynamics simulations, our results show that a minimal encounter duration is required for 2D association; an energy landscape featuring a rough initial part might be a reasonable way of accounting for this. By systematically varying the temperature of our experiments, we evaluate roughness at 2kBT, in the range of previously proposed rough parts of landscapes models during dissociation. PMID:27731375

  4. A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.

    PubMed

    Limozin, Laurent; Bongrand, Pierre; Robert, Philippe

    2016-10-12

    Antibodies and B cell receptors often bind their antigen at cell-cell interface while both molecular species are surface-bound, which impacts bond kinetics and function. Despite the description of complex energy landscapes for dissociation kinetics which may also result in significantly different association kinetics, surface-bound molecule (2D) association kinetics usually remain described by an on-rate due to crossing of a single free energy barrier, and few experimental works have measured association kinetics under conditions implying force and two-dimensional relative ligand-receptor motion. We use a new laminar flow chamber to measure 2D bond formation with systematic variation of the distribution of encounter durations between antigen and antibody, in a range from 0.1 to 10 ms. Under physiologically relevant forces, 2D association is 100-fold slower than 3D association as studied by surface plasmon resonance assays. Supported by brownian dynamics simulations, our results show that a minimal encounter duration is required for 2D association; an energy landscape featuring a rough initial part might be a reasonable way of accounting for this. By systematically varying the temperature of our experiments, we evaluate roughness at 2k B T, in the range of previously proposed rough parts of landscapes models during dissociation.

  5. METHODOLOGIES FOR SCREENING AND ASSESSING INTRUSION OF CONTAMINATED VAPORS INTO BUILDINGS

    EPA Science Inventory

    Current investigations into the risk to human health associated with Brownfield redevelopment may be overlooking an important exposure pathway as a result of vapor intrusion into buildings. Persons inhabiting or employed in new or existing structures built over volatile organic c...

  6. TECHNICAL APPROACHES TO CHARACTERIZING AND CLEANING UP BROWNFIELDS SITES: PULP AND PAPER MILLS

    EPA Science Inventory

    This guidance document gives assistance to communities, decision-makers, states and municipalities, academia, and the private sector to address issues related to the redevelopment of Brownfields sites, specifically pulp and paper mills sites. The document helps users to understan...

  7. Redeveloping Early Childhood Education: A Response to Kessler.

    ERIC Educational Resources Information Center

    Bredekamp, Sue

    1991-01-01

    Provides background information on the development of NAEYC's position statements on developmentally appropriate practice and clarifies assumptions made by Kessler and others about NAEYC's positions. Offers justification for the developmentalist perspective and suggests an alternative argument for appropriate practice that draws on the strengths…

  8. 29 CFR 31.3 - General standards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... which resulted in limiting participation by persons of a particular race, color or national origin. (7..., color, or national origin. (2) Manpower Development and Training Act, work-incentive under Social... respect to any trainee or enrollee under the Manpower Development and Training Act, Area Redevelopment Act...

  9. 29 CFR 31.3 - General standards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... which resulted in limiting participation by persons of a particular race, color or national origin. (7..., color, or national origin. (2) Manpower Development and Training Act, work-incentive under Social... respect to any trainee or enrollee under the Manpower Development and Training Act, Area Redevelopment Act...

  10. 29 CFR 31.3 - General standards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... which resulted in limiting participation by persons of a particular race, color or national origin. (7..., color, or national origin. (2) Manpower Development and Training Act, work-incentive under Social... respect to any trainee or enrollee under the Manpower Development and Training Act, Area Redevelopment Act...

  11. 29 CFR 31.3 - General standards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... which resulted in limiting participation by persons of a particular race, color or national origin. (7..., color, or national origin. (2) Manpower Development and Training Act, work-incentive under Social... respect to any trainee or enrollee under the Manpower Development and Training Act, Area Redevelopment Act...

  12. 29 CFR 31.3 - General standards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... which resulted in limiting participation by persons of a particular race, color or national origin. (7..., color, or national origin. (2) Manpower Development and Training Act, work-incentive under Social... respect to any trainee or enrollee under the Manpower Development and Training Act, Area Redevelopment Act...

  13. To Image...or Not to Image?

    ERIC Educational Resources Information Center

    Bruley, Karina

    1996-01-01

    Provides a checklist of considerations for installing document image processing with an electronic document management system. Other topics include scanning; indexing; the image file life cycle; benefits of imaging; document-driven workflow; and planning for workplace changes like postsorting, creating a scanning room, redeveloping job tasks and…

  14. The University as a Land Developer.

    ERIC Educational Resources Information Center

    Fink, Ira

    1983-01-01

    To assist universities in developing or redeveloping surplus campus or endowment property, these factors in the process are outlined and discussed: development decisions, working with a developer, ground leasing, unrelated taxable income issues, creating a university land management office, some recent experiences, and research and development…

  15. 32 CFR 176.5 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... BASE CLOSURE COMMUNITIES AND COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 176... Liability Act (42 U.S.C. 9601 et seq.). Communities in the vicinity of the installation. The communities... serving in that capacity, the communities in the vicinity of the installation are deemed to be those...

  16. 32 CFR 176.5 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... BASE CLOSURE COMMUNITIES AND COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 176... Liability Act (42 U.S.C. 9601 et seq.). Communities in the vicinity of the installation. The communities... serving in that capacity, the communities in the vicinity of the installation are deemed to be those...

  17. 75 FR 81239 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-27

    ... civilian job creation and type of redevelopment at the former military installations. The respondents to... generate jobs as military activity diminishes and to serve as a clearinghouse for reuse data. Affected... DEPARTMENT OF DEFENSE Office of the Secretary [Docket ID DOD-2010-OS-0170] Proposed Collection...

  18. SUSTAINABLE REUSE AND REVITALIZATION OF POTENTIALLY CONTAMINATED SITES CD

    EPA Science Inventory

    The goal of this CD is to demonstrate and discuss principles of sustainable reuse and revitalization through examples of sustainable practices as applied to redevelopment in both the USA and Germany, emphasizing urban development and sites at the urban/rural fringe or interface.

  19. Applying geologic sensitivity analysis to environmental risk management: The financial implications

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rogers, D.T.

    The financial risks associated with environmental contamination can be staggering and are often difficult to identify and accurately assess. Geologic sensitivity analysis is gaining recognition as a significant and useful tool that can empower the user with crucial information concerning environmental risk management and brownfield redevelopment. It is particularly useful when (1) evaluating the potential risks associated with redevelopment of historical industrial facilities (brownfields) and (2) planning for future development, especially in areas of rapid development because the number of potential contaminating sources often increases with an increase in economic development. An examination of the financial implications relating to geologicmore » sensitivity analysis in southeastern Michigan from numerous case studies indicate that the environmental cost of contamination may be 100 to 1,000 times greater at a geologically sensitive location compared to the least sensitive location. Geologic sensitivity analysis has demonstrated that near-surface geology may influence the environmental impact of a contaminated site to a greater extent than the amount and type of industrial development.« less

  20. Simulations of Magnetic Reconnection - Kinetic Mechanisms Underlying the Fluid Description of Ions

    NASA Technical Reports Server (NTRS)

    Aunai, icolas; Belmont, Gerard; Smets, Roch

    2012-01-01

    Because of its ability to transfer the energy stored in magnetic field together with the breaking of the flux freezing constraint, magnetic reconnection is considered as one of the most important phenomena in plasma physics. When it happens in a collision less environment such as the terrestrial magnetosphere, it should a priori be modelled with in the framework of kinetic physics. The evidence of kinetic features has incidentally for a long time, been shown by researchers with the help of both numerical simulations and satellite observations. However, most of our understanding of the process comes from the more intuitive fluid interpretation with simple closure hypothesis which do not include kinetic effects. To what extent are these two separate descriptions of the same phenomenon related? What is the role of kinetic effects in the averaged/fluid dynamics of reconnection? This thesis addresses these questions for the proton population in the particular case of anti parallel merging with the help of 2D Hybrid simulations. We show that one can not assume, as is usually done, that the acceleration of the proton flow is only due to the Laplace force. Our results show, for symmetric and asymmetric connection, the importance of the pressure force, opposed to the electric one on the separatrices, in the decoupling region. In the symmetric case, we emphasize the kinetic origin of this force by analyzing the proton distribution functions and explain their structure by studying the underlying particle dynamics. Protons, as individual particles, are shown to bounce in the electric potential well created by the Hall effect. The spatial divergence of this well results in a mixing in phase space responsible for the observed structure of the pressure tensor. A detailed energy budget analysis confirms the role of the pressure force for the acceleration; but, contrary to what is sometimes assumed, it also reveals that the major part of the incoming Poynting flux is transferred to the thermal energy flux rather than to the convective kinetic energy flux, although the latter is generally supposed dominant. In the symmetric case, we propose the pressure tensor to be an additional proxy of the ion decoupling region in satellite data and verify this suggestion by studying a reconnection event encountered by the Cluster spacecrafts. Finally, the last part of this thesis is devoted to the study of the kinetic structure of asymmetric tangential current sheets where connection can develop. This theoretical part consists in finding a steady state solution to the Vlasov-Maxwell system for the protons in such a configuration. We present the theory and its first confrontation to numerical tests.

  1. Ground Contact Modeling for the Morpheus Test Vehicle Simulation

    NASA Technical Reports Server (NTRS)

    Cordova, Luis

    2014-01-01

    The Morpheus vertical test vehicle is an autonomous robotic lander being developed at Johnson Space Center (JSC) to test hazard detection technology. Because the initial ground contact simulation model was not very realistic, it was decided to improve the model without making it too computationally expensive. The first development cycle added capability to define vehicle attachment points (AP) and to keep track of their states in the lander reference frame (LFRAME). These states are used with a spring damper model to compute an AP contact force. The lateral force is then overwritten, if necessary, by the Coulomb static or kinetic friction force. The second development cycle added capability to use the PolySurface class as the contact surface. The class can load CAD data in STL (Stereo Lithography) format, and use the data to compute line of sight (LOS) intercepts. A polygon frame (PFRAME) is computed from the facet intercept normal and used to convert the AP state to PFRAME. Three flat plane tests validate the transitions from kinetic to static, static to kinetic, and vertical impact. The hazardous terrain test will be used to test for visual reasonableness. The improved model is numerically inexpensive, robust, and produces results that are reasonable.

  2. Ground Contact Modeling for the Morpheus Test Vehicle Simulation

    NASA Technical Reports Server (NTRS)

    Cordova, Luis

    2013-01-01

    The Morpheus vertical test vehicle is an autonomous robotic lander being developed at Johnson Space Center (JSC) to test hazard detection technology. Because the initial ground contact simulation model was not very realistic, it was decided to improve the model without making it too computationally expensive. The first development cycle added capability to define vehicle attachment points (AP) and to keep track of their states in the lander reference frame (LFRAME). These states are used with a spring damper model to compute an AP contact force. The lateral force is then overwritten, if necessary, by the Coulomb static or kinetic friction force. The second development cycle added capability to use the PolySurface class as the contact surface. The class can load CAD data in STL (Stereo Lithography) format, and use the data to compute line of sight (LOS) intercepts. A polygon frame (PFRAME) is computed from the facet intercept normal and used to convert the AP state to PFRAME. Three flat plane tests validate the transitions from kinetic to static, static to kinetic, and vertical impact. The hazardous terrain test will be used to test for visual reasonableness. The improved model is numerically inexpensive, robust, and produces results that are reasonable.

  3. Hip joint kinetics in the table tennis topspin forehand: relationship to racket velocity.

    PubMed

    Iino, Yoichi

    2018-04-01

    The purpose of this study was to determine hip joint kinetics during a table tennis topspin forehand, and to investigate the relationship between the relevant kinematic and kinetic variables and the racket horizontal and vertical velocities at ball impact. Eighteen male advanced table tennis players hit cross-court topspin forehands against backspin balls. The hip joint torque and force components around the pelvis coordinate system were determined using inverse dynamics. Furthermore, the work done on the pelvis by these components was also determined. The peak pelvis axial rotation velocity and the work done by the playing side hip pelvis axial rotation torque were positively related to the racket horizontal velocity at impact. The sum of the work done on the pelvis by the backward tilt torques and the upward joint forces was positively related to the racket vertical velocity at impact. The results suggest that the playing side hip pelvis axial rotation torque exertion is important for acquiring a high racket horizontal velocity at impact. The pelvis backward tilt torques and upward joint forces at both hip joints collectively contribute to the generation of the racket vertical velocity, and the mechanism for acquiring the vertical velocity may vary among players.

  4. Kinetic effects on double hysteresis in spin crossover molecular magnets analyzed with first order reversal curve diagram technique

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stan, Raluca-Maria; Gaina, Roxana; Enachescu, Cristian, E-mail: cristian.enachescu@uaic.ro, E-mail: radu.tanasa@uaic.ro

    2015-05-07

    In this paper, we analyze two types of hysteresis in spin crossover molecular magnets compounds in the framework of the First Order Reversal Curve (FORC) method. The switching between the two stable states in these compounds is accompanied by hysteresis phenomena if the intermolecular interactions are higher than a threshold. We have measured the static thermal hysteresis (TH) and the kinetic light induced thermal hysteresis (LITH) major loops and FORCs for the polycrystalline Fe(II) spin crossover compound [Fe{sub 1−x}Zn{sub x}(bbtr){sub 3}](ClO{sub 4}){sub 2} (bbtr = 1,4-di(1,2,3-triazol-1-yl)butane), either in a pure state (x = 0) or doped with Zn ions (x = 0.33) considering different sweeping rates.more » Here, we use this method not only to infer the domains distribution but also to disentangle between kinetic and static components of the LITH and to estimate the changes in the intermolecular interactions introduced by dopants. We also determined the qualitative relationship between FORC distributions measured for TH and LITH.« less

  5. A kinetic analysis of manual wheelchair propulsion during start-up on select indoor and outdoor surfaces.

    PubMed

    Koontz, Alicia M; Cooper, Rory A; Boninger, Michael L; Yang, Yusheng; Impink, Bradley G; van der Woude, Lucas H V

    2005-01-01

    The objective of this study was to conduct a kinetic analysis of manual wheelchair propulsion during start-up on select indoor and outdoor surfaces. Eleven manual wheelchairs were fitted with a SMART(Wheel) and their users were asked to push on a course consisting of high- and low-pile carpet, indoor tile, interlocking concrete pavers, smooth level concrete, grass, hardwood flooring, and a sidewalk with a 5-degree grade. Peak resultant force, wheel torque, mechanical effective force, and maximum resultant force rate of rise were analyzed during start-up for each surface and normalized relative to their steady-state values on the smooth level concrete. Additional variables included peak velocity, distance traveled, and number of strokes in the first 5 s of the trial. We compared biomechanical data between surfaces using repeated-measures mixed models and paired comparisons with a Bonferroni adjustment. Applied resultant force (p = 0.0154), wheel torque (p < 0.0001), and mechanical effective force (p = 0.0047) were significantly different between surfaces. The kinetic values for grass, interlocking pavers, and ramp ascent were typically higher compared with tile, wood, smooth level concrete, and high- and low-pile carpet. Users were found to travel shorter distances up the ramp and across grass (p < 0.0025) and had a higher stroke count on the ramp (p = 0.0124). While peak velocity was not statistically different, average velocity was slower for the ramp and grass, which indicates greater wheelchair/user deceleration between strokes. The differences noted between surfaces highlight the importance of evaluating wheelchair propulsion ability over a range of surfaces.

  6. Numerical study of hydrophobic micron particle's impaction on liquid surface

    NASA Astrophysics Data System (ADS)

    Ji, Bingqiang; Song, Qiang; Yao, Qiang

    2017-07-01

    In this study, a simulation method is established for the impaction of micron particles on liquid surfaces, by which the processes of two impaction modes (submergence and oscillation) are studied. The submergence is found to go through three stages, each of which shows different characteristics of particle velocity and gas-liquid interface variance. The dominant forces of the early and late times of the submergence mode are hydrodynamic force and surface tension, respectively, the accumulated work of which is in the same order. The lost particle kinetic energy is converted to the surface energy of the interfaces, the internal energy and the kinetic energy of fluids. The primary part of the oscillation is the first cycle, and the characteristics of its sinking process are similar to that of the submergence. In the reverting stage, the particle rising velocity increases first and then decreases, and the cavity retracts until the gas-liquid interface flattens. The dominant forces of the early and late times of the reverting stage are surface tension and hydrodynamic force, respectively. The positive accumulated work of surface tension on the particle is considerably limited due to the large contact angle hysteresis at the early times of the reverting stage. The negative accumulated work of the hydrodynamic force on the particle at the late times causes a fast decrease in particle kinetic energy, which leads to particle floating on the gas-liquid interface. The results are helpful in understanding the mechanism of micron particle impaction and developing the prediction method of attachment efficiency.

  7. Kinematics and Kinetics of Squats, Drop Jumps and Imitation Jumps of Ski Jumpers

    PubMed Central

    Pauli, Carole A.; Keller, Melanie; Ammann, Fabian; Hübner, Klaus; Lindorfer, Julia; Taylor, William R.

    2016-01-01

    Abstract Pauli, CA, Keller, M, Ammann, F, Hübner, K, Lindorfer, J, Taylor, WR, and Lorenzetti, S. Kinematics and kinetics of squats, drop jumps and imitation jumps of ski jumpers. J Strength Cond Res 30(3): 643–652, 2016—Squats, drop jumps, and imitation jumps are commonly used training exercises in ski jumping to enhance maximum force, explosive force, and sport-specific skills. The purpose of this study was to evaluate the kinetics and kinematics of training exercises in ski jumping and to find objective parameters in training exercises that most correlate with the competition performance of ski jumpers. To this end, barbell squats, drop jumps, and imitation jumps were measured in a laboratory environment for 10 elite ski jumpers. Force and motion data were captured, and the influence of maximum vertical force, force difference, vertical take-off velocity, knee moments, knee joint power, and a knee valgus/varus index was evaluated and correlated with their season jump performance. The results indicate that, especially for the imitation jumps, a good correlation exists between the vertical take-off velocity and the personal jump performance on the hill (R = 0.718). Importantly, however, the more the athletes tended toward a valgus knee alignment during the measured movements, the worse their performance (R = 0.729 imitation jumps; R = 0.685 squats). Although an evaluation of the athletes' lower limb alignment during competitive jumping on the hill is still required, these preliminary data suggest that performance training should additionally concentrate on improving knee alignment to increase ski jumping performance. PMID:26418370

  8. Statolith sedimentation kinetics and force transduction to the cortical endoplasmic reticulum in gravity-sensing Arabidopsis columella cells.

    PubMed

    Leitz, Guenther; Kang, Byung-Ho; Schoenwaelder, Monica E A; Staehelin, L Andrew

    2009-03-01

    The starch statolith hypothesis of gravity sensing in plants postulates that the sedimentation of statoliths in specialized statocytes (columella cells) provides the means for converting the gravitational potential energy into a biochemical signal. We have analyzed the sedimentation kinetics of statoliths in the central S2 columella cells of Arabidopsis thaliana. The statoliths can form compact aggregates with gap sizes between statoliths approaching <30 nm. Significant intra-aggregate sliding motions of individual statoliths suggest a contribution of hydrodynamic forces to the motion of statoliths. The reorientation of the columella cells accelerates the statoliths toward the central cytoplasm within <1 s of reorientation. During the subsequent sedimentation phase, the statoliths tend to move at a distance to the cortical endoplasmic reticulum (ER) boundary and interact only transiently with the ER. Statoliths moved by laser tweezers against the ER boundary experience an elastic lift force upon release from the optical trap. High-resolution electron tomography analysis of statolith-to-ER contact sites indicate that the weight of statoliths is sufficient to locally deform the ER membranes that can potentially activate mechanosensitive ion channels. We suggest that in root columella cells, the transduction of the kinetic energy of sedimenting statoliths into a biochemical signal involves a combination of statolith-driven motion of the cytosol, statolith-induced deformation of the ER membranes, and a rapid release of kinetic energy from the ER during reorientation to activate mechanosensitive sites within the central columella cells.

  9. Statolith Sedimentation Kinetics and Force Transduction to the Cortical Endoplasmic Reticulum in Gravity-Sensing Arabidopsis Columella Cells[W][OA

    PubMed Central

    Leitz, Guenther; Kang, Byung-Ho; Schoenwaelder, Monica E.A.; Staehelin, L. Andrew

    2009-01-01

    The starch statolith hypothesis of gravity sensing in plants postulates that the sedimentation of statoliths in specialized statocytes (columella cells) provides the means for converting the gravitational potential energy into a biochemical signal. We have analyzed the sedimentation kinetics of statoliths in the central S2 columella cells of Arabidopsis thaliana. The statoliths can form compact aggregates with gap sizes between statoliths approaching <30 nm. Significant intra-aggregate sliding motions of individual statoliths suggest a contribution of hydrodynamic forces to the motion of statoliths. The reorientation of the columella cells accelerates the statoliths toward the central cytoplasm within <1 s of reorientation. During the subsequent sedimentation phase, the statoliths tend to move at a distance to the cortical endoplasmic reticulum (ER) boundary and interact only transiently with the ER. Statoliths moved by laser tweezers against the ER boundary experience an elastic lift force upon release from the optical trap. High-resolution electron tomography analysis of statolith-to-ER contact sites indicate that the weight of statoliths is sufficient to locally deform the ER membranes that can potentially activate mechanosensitive ion channels. We suggest that in root columella cells, the transduction of the kinetic energy of sedimenting statoliths into a biochemical signal involves a combination of statolith-driven motion of the cytosol, statolith-induced deformation of the ER membranes, and a rapid release of kinetic energy from the ER during reorientation to activate mechanosensitive sites within the central columella cells. PMID:19276442

  10. Nonlinear Drift-Kinetic Equation in the Presence of a Circularly Polarized Wave

    NASA Technical Reports Server (NTRS)

    Khazanov, G. V.; Krivorutsky, E. N.; Six, N. Frank (Technical Monitor)

    2002-01-01

    Equations of the single particle motion and nonlinear kinetic equation for plasma in the presence of a circularly polarized wave of arbitrary frequency in the drift approximation are presented. The nonstationarity and inhomogeneity of the plasma-wave system are taken into account. The time dependent part of the ponderomotive force is discussed.

  11. Being Careful with PASCO's Kinetic Friction Experiment: Uncovering Pre-Sliding Displacement?

    ERIC Educational Resources Information Center

    Lawlor, T. M.

    2008-01-01

    The widely used PASCO laboratory equipment is an excellent way to introduce students to many topics in physics. In one case, PASCO's equipment may be too good! Various experiments exist for calculating the kinetic coefficient of friction by measuring the acceleration of a sliding object under some constant force. With ever more accurate equipment,…

  12. Friction between various self-ligating brackets and archwire couples during sliding mechanics.

    PubMed

    Stefanos, Sennay; Secchi, Antonino G; Coby, Guy; Tanna, Nipul; Mante, Francis K

    2010-10-01

    The aim of this study was to evaluate the frictional resistance between active and passive self-ligating brackets and 0.019 × 0.025-in stainless steel archwire during sliding mechanics by using an orthodontic sliding simulation device. Maxillary right first premolar active self-ligating brackets In-Ovation R, In-Ovation C (both, GAC International, Bohemia, NY), and SPEED (Strite Industries, Cambridge, Ontario, Canada), and passive self-ligating brackets SmartClip (3M Unitek, Monrovia, Calif), Synergy R (Rocky Mountain Orthodontics, Denver, Colo), and Damon 3mx (Ormco, Orange, Calif) with 0.022-in slots were used. Frictional force was measured by using an orthodontic sliding simulation device attached to a universal testing machine. Each bracket-archwire combination was tested 30 times at 0° angulation relative to the sliding direction. Statistical comparisons were performed with 1-way analysis of variance (ANOVA) followed by Dunn multiple comparisons. The level of statistical significance was set at P <0.05. The Damon 3mx brackets had significantly the lowest mean static frictional force (8.6 g). The highest mean static frictional force was shown by the SPEED brackets (83.1 g). The other brackets were ranked as follows, from highest to lowest, In-Ovation R, In-Ovation C, SmartClip, and Synergy R. The mean static frictional forces were all statistically different. The ranking of the kinetic frictional forces of bracket-archwire combinations was the same as that for static frictional forces. All bracket-archwire combinations showed significantly different kinetic frictional forces except SmartClip and In-Ovation C, which were not significantly different from each other. Passive self-ligating brackets have lower static and kinetic frictional resistance than do active self-ligating brackets with 0.019 × 0.025-in stainless steel wire. Copyright © 2010 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

  13. Influence of long-term in vivo exposure, debris accumulation and archwire material on friction force among different types of brackets and archwires couples.

    PubMed

    Mezeg, Uroš; Primožic, Jasmina

    2017-11-30

    The aim was to assess the influence of long-term in vivo exposure, debris accumulation and archwire material on static and kinetic friction force among different types of brackets and archwires couples. Friction testing was performed among four lower incisors' brackets, conventional and self-ligating (SL), coupled with either nickel-titanium or stainless steel archwires, as-received and in vivo exposed in 18 subjects. The friction testing was performed for a sliding distance of 14 mm at a speed of 10 mm/min, with a starting force of 0.2 N. Wear and quantitative assessment of debris accumulation was performed on pictures of brackets obtained using a scanning electron microscope. Non parametric tests were used for statistical analysis. Only bracket type, but not exposure duration, amount of debris accumulation, archwire material or their manufacturer, was significantly correlated with both static (rho = 0.602, P < 0.001) and kinetic (rho = 0.584, P < 0.001) friction force. Within each bracket type no significant difference was observed between as-received and in vivo exposed brackets for any friction parameter except for the SL brackets in which significantly higher static and kinetic (P = 0.001, at least) friction forces were seen in in vivo exposed SL brackets (164.9 cN and 217.63 cN, respectively) in comparison with as-received SL brackets (19.69 cN and 55.72 cN, respectively). The frictional testing was performed in the dry condition which might have influenced the results. A significant correlation was seen between friction force and bracket type, while treatment duration, amount of debris accumulation, archwire material or their manufacturer was not significantly correlated to it. Nevertheless, higher friction forces were measured among in vivo aged SL brackets in comparison with as-received ones. © The Author 2017. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved. For permissions, please email: journals.permissions@oup.com

  14. Fractal reaction kinetics.

    PubMed

    Kopelman, R

    1988-09-23

    Classical reaction kinetics has been found to be unsatisfactory when the reactants are spatially constrained on the microscopic level by either walls, phase boundaries, or force fields. Recently discovered theories of heterogeneous reaction kinetics have dramatic consequences, such as fractal orders for elementary reactions, self-ordering and self-unmixing of reactants, and rate coefficients with temporal "memories." The new theories were needed to explain the results of experiments and supercomputer simulations of reactions that were confined to low dimensions or fractal dimensions or both. Among the practical examples of "fractal-like kinetics" are chemical reactions in pores of membranes, excitation trapping in molecular aggregates, exciton fusion in composite materials, and charge recombination in colloids and clouds.

  15. 50 CFR 217.202 - Permissible methods of taking.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false Permissible methods of taking. 217.202 Section 217.202 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC... Redevelopment Project § 217.202 Permissible methods of taking. (a) Under Letters of Authorization issued...

  16. 50 CFR 217.202 - Permissible methods of taking.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false Permissible methods of taking. 217.202 Section 217.202 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC... Redevelopment Project § 217.202 Permissible methods of taking. (a) Under Letters of Authorization issued...

  17. U.S. - GERMAN BILATERAL WORKING GROUP WORKSHOP ON: ECONOMIC TOOLS FOR SUSTAINABLE BROWNFIELDS REDEVELOPMENT

    EPA Science Inventory

    This CD-ROM contains information from a two-day workshop discussing innovative brownfields financing and economic strategies in the United States and Germany. A special emphasis was given to the identification of advantages and disadvantages of different financial tools, economi...

  18. SMARTE: SUSTAINABLE MANAGEMENT APPROACHES AND REVITALIZATION TOOLS-ELECTRONIC (BELFAST, IRELAND)

    EPA Science Inventory

    The U.S.-German Bilateral Working Group is developing Site-specific Management Approaches and Redevelopment Tools (SMART). In the U.S., the SMART compilation is housed in a web-based, decision support tool called SMARTe. All tools within SMARTe that are developed specifically for...

  19. National Stormwater Calculator: Low Impact Development Stormwater Control Cost Estimation Programming & Future Enhancements - Presentation

    EPA Science Inventory

    The National Stormwater Calculator (NSC) makes it easy to estimate runoff reduction when planning a new development or redevelopment site with low impact development (LID) stormwater controls. The Calculator is currently deployed as a Windows desktop application. The NSC is organ...

  20. National Stormwater Calculator: Low Impact Development Stormwater Control Cost Estimation Programming & Future Enhancements - abstract

    EPA Science Inventory

    The National Stormwater Calculator (NSC) makes it easy to estimate runoff reduction when planning a new development or redevelopment site with low impact development (LID) stormwater controls. The Calculator is currently deployed as a Windows desktop application. The Calculator i...

  1. Where Are the Grounds for Grounded Theory? A Troubled Empirical Methodology Meets Wittgenstein

    ERIC Educational Resources Information Center

    James, Fiona

    2018-01-01

    This article provides a critical exposition of the epistemological underpinnings of a recent redevelopment of Grounded Theory (GT) methodology, "Constructivist" GT. Although proffered as freed from the "objectivist" tenets of the original version, critical examination exposes the essentialism threaded through its integral…

  2. Chaos of Textures or "Tapisserie"? A Model for Creative Teacher Education Curriculum Design

    ERIC Educational Resources Information Center

    Simon, Sue E.

    2013-01-01

    A tapestry or "tapisserie" methodology, inspired by Denzin and Lincoln's "bricolage" methodology (2000), emerged during the complex task of re-developing teacher education programs at the University of the Sunshine Coast, Queensland, Australia. "Tapisserie" methodology highlights the pivotal task of determining…

  3. SITE-SPECIFIC MANAGEMENT APPROACHES AND REDEVELOPMENT TOOLS (SMART) GUIDANCE AND SMARTE (ELECTRONIC)

    EPA Science Inventory

    The United States Environmental Protection Agency (EPA) and the German Federal Ministry of Eductation and Research B(BMBF) continues an ongoing partnership to gain an understanding of each other's approach to the cleanup of chemical contamination in order to protect human health ...

  4. ENVIRONMENTAL RISK ASSESSMENT/COMMUNICATION TOOLS FOR REVITALIZATION OF POTENTIALLY CONTAMINATED SITES CD

    EPA Science Inventory

    Revitalization of potentially contaminated sites is often at a disadvantage compared to greenfield development. Apart from the lower cost of land in rural areas, redevelopment is very often still seen as a rather complex time- and cost-consuming process. Additionally, the potenti...

  5. Elementary Design Challenges

    ERIC Educational Resources Information Center

    Gerlach, Jonathan W.

    2010-01-01

    How many of our students come to the classroom with little background knowledge about the world around them and how things work? To help students develop conceptual understanding and explore the design process, the author brought the NASA "Engineering Design Challenges" program to his school district, redeveloped for elementary students. In this…

  6. 50 CFR 217.201 - Effective dates.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Effective dates. 217.201 Section 217.201 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION... Redevelopment Project § 217.201 Effective dates. Regulations in this subpart are effective from July 15, 2009...

  7. 76 FR 39386 - Taking and Importing Marine Mammals; Taking Marine Mammals Incidental to the Port of Anchorage...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-06

    ... graduate and undergraduate marine biology students conducted approximately 600 hours of scientific... Importing Marine Mammals; Taking Marine Mammals Incidental to the Port of Anchorage Marine Terminal Redevelopment Project AGENCY: National Marine Fisheries Service, National Oceanic and Atmospheric Administration...

  8. PRES 2013: Results from the Postgraduate Research Experience Survey

    ERIC Educational Resources Information Center

    Bennett, Paul; Turner, Gosia

    2013-01-01

    This document outlines the results of the "2013 Postgraduate Research Experience Survey" ("PRES"), where 48,401 replies were received from 122 participating institutions. Redeveloped for 2013, our biennial survey is the only national survey to gather insight from postgraduate research students about their learning and…

  9. 78 FR 56980 - Muscle Shoals Reservation Redevelopment, Colbert County, Alabama

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-16

    ... protect the integrated operation of TVA reservoir and power systems, to provide for appropriate public use and enjoyment of the reservoir system, and to provide for continuing economic growth in the Tennessee... specific use agreements, such as easements. Because of environmental and reservoir operations constraints...

  10. SWMM 5 - A Case Study of Model Re-Development

    EPA Science Inventory

    By the turn of the 21st century the U.S. Environmental Protection Agency’s (EPA) Storm Water Management Model (SWMM) already had a 30-year history of extensive use throughout the world for analyzing complex hydrologic, hydraulic, and water quality problems related to urban draina...

  11. 2015 Revolving Loan Fund Supplemental Awards for Brownfields and Land Revitalization in New England

    EPA Pesticide Factsheets

    Communities in CT, ME, MA and VT will receive additional grants of $4.3 million in revolving loan fund (RLF) supplemental funding to carry out cleanup and redevelopment projects at contaminated Brownfields properties. See a list of recipients for 2015.

  12. Ready for Reuse (RfR) Determinations at Superfund Sites

    EPA Pesticide Factsheets

    A Ready for Reuse (RfR) determination is a redevelopment tool that EPA has created to provide information that a site is “ready for reuse” and will remain protective for that use, so long as any use limitations established by EPA continue to be met.

  13. SITE-SPECIFIC MANAGEMENT APPROACHES AND REDEVELOPMENT TOOLS (SMART) FOR A PRESENTATION ON OCTOBER 2003.

    EPA Science Inventory

    The United States Environmental Protection Agency (EPA) and the German Federal Ministry of Education and Research (BMBF) created a Bilateral Working Group in 1990 to gain a better understanding of each country's efforts in developing and demonstrating remedial technologies (Phase...

  14. [Upper extremity kinetics and energy expenditure during walker-assisted gait in children with cerebral palsy].

    PubMed

    Konop, Katherine A; Strifling, Kelly M B; Wang, Mei; Cao, Kevin; Eastwood, Daniel; Jackson, Scott; Ackman, Jeffrey; Altiok, Haluk; Schwab, Jeffrey; Harris, Gerald F

    2009-01-01

    We evaluated the relationships between upper extremity (UE) kinetics and the energy expenditure index during anterior and posterior walker-assisted gait in children with spastic diplegic cerebral palsy (CP). Ten children (3 boys, 7 girls; mean age 12.1 years; range 8 to 18 years) with spastic diplegic CP, who ambulated with a walker underwent gait analyses that included UE kinematics and kinetics. Upper extremity kinetics were obtained using instrumented walker handles. Energy expenditure index was obtained using the heart rate method (EEIHR) by subtracting resting heart rate from walking heart rate, and dividing by the walking speed. Correlations were sought between the kinetic variables and the EEIHR and temporal and stride parameters. In general, anterior walker use was associated with a higher EEIHR. Several kinetic variables correlated well with temporal and stride parameters, as well as the EEIHR. All of the significant correlations (r>0.80; p<0.005) occurred during anterior walker use and involved joint reaction forces (JRF) rather than moments. Some variables showed multiple strong correlations during anterior walker use, including the medial JRF in the wrist, the posterior JRF in the elbow, and the inferior and superior JRFs in the shoulder. The observed correlations may indicate a relationship between the force used to advance the body forward within the walker frame and an increased EEIHR. More work is needed to refine the correlations, and to explore relationships with other variables, including the joint kinematics.

  15. Quantification of upper limb kinetic asymmetries in front crawl swimming.

    PubMed

    Morouço, Pedro G; Marinho, Daniel A; Fernandes, Ricardo J; Marques, Mário C

    2015-04-01

    This study aimed at quantifying upper limb kinetic asymmetries in maximal front crawl swimming and to examine if these asymmetries would affect the contribution of force exertion to swimming performance. Eighteen high level male swimmers with unilateral breathing patterns and sprint or middle distance specialists, volunteered as participants. A load-cell was used to quantify the forces exerted in water by completing a 30s maximal front crawl tethered swimming test and a maximal 50 m free swimming was considered as a performance criterion. Individual force-time curves were obtained to calculate the mean and maximum forces per cycle, for each upper limb. Following, symmetry index was estimated and breathing laterality identified by questionnaire. Lastly, the pattern of asymmetries along the test was estimated for each upper limb using linear regression of peak forces per cycle. Asymmetrical force exertion was observed in the majority of the swimmers (66.7%), with a total correspondence of breathing laterality opposite to the side of the force asymmetry. Forces exerted by the dominant upper limb presented a higher decrease than from the non-dominant. Very strong associations were found between exerted forces and swimming performance, when controlling the isolated effect of symmetry index. Results point that force asymmetries occur in the majority of the swimmers, and that these asymmetries are most evident in the first cycles of a maximum bout. Symmetry index stood up as an influencing factor on the contribution of tethered forces over swimming performance. Thus, to some extent, a certain degree of asymmetry is not critical for short swimming performance. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK210.

    PubMed

    Inoue, Takahiro; Kobirumaki-Shimozawa, Fuyu; Kagemoto, Tatsuya; Fujii, Teruyuki; Terui, Takako; Kusakari, Yoichiro; Hongo, Kenichi; Morimoto, Sachio; Ohtsuki, Iwao; Hashimoto, Kazuhiro; Fukuda, Norio

    2013-10-01

    It has been reported that the Frank-Starling mechanism is coordinately regulated in cardiac muscle via thin filament "on-off" equilibrium and titin-based lattice spacing changes. In the present study, we tested the hypothesis that the deletion mutation ΔK210 in the cardiac troponin T gene shifts the equilibrium toward the "off" state and accordingly attenuate the sarcomere length (SL) dependence of active force production, via reduced cross-bridge formation. Confocal imaging in isolated hearts revealed that the cardiomyocytes were enlarged, especially in the longitudinal direction, in ΔK210 hearts, with striation patterns similar to those in wild type (WT) hearts, suggesting that the number of sarcomeres is increased in cardiomyocytes but the sarcomere length remains unaltered. For analysis of the SL dependence of active force, skinned muscle preparations were obtained from the left ventricle of WT and knock-in (ΔK210) mice. An increase in SL from 1.90 to 2.20μm shifted the mid-point (pCa50) of the force-pCa curve leftward by ~0.21pCa units in WT preparations. In ΔK210 muscles, Ca(2+) sensitivity was lower by ~0.37pCa units, and the SL-dependent shift of pCa50, i.e., ΔpCa50, was less pronounced (~0.11pCa units), with and without protein kinase A treatment. The rate of active force redevelopment was lower in ΔK210 preparations than in WT preparations, showing blunted thin filament cooperative activation. An increase in thin filament cooperative activation upon an increase in the fraction of strongly bound cross-bridges by MgADP increased ΔpCa50 to ~0.21pCa units. The depressed Frank-Starling mechanism in ΔK210 hearts is the result of a reduction in thin filament cooperative activation. © 2013.

  17. Correlation of Shoulder and Elbow Kinetics With Ball Velocity in Collegiate Baseball Pitchers.

    PubMed

    Post, Eric G; Laudner, Kevin G; McLoda, Todd A; Wong, Regan; Meister, Keith

    2015-06-01

    Throwing a baseball is a dynamic and violent act that places large magnitudes of stress on the shoulder and elbow. Specific injuries at the elbow and glenohumeral joints have been linked to several kinetic variables throughout the throwing motion. However, very little research has directly examined the relationship between these kinetic variables and ball velocity. To examine the correlation of peak ball velocity with elbow-valgus torque, shoulder external-rotation torque, and shoulder-distraction force in a group of collegiate baseball pitchers. Cross-sectional study. Motion-analysis laboratory. Sixty-seven asymptomatic National Collegiate Athletic Association Division I baseball pitchers (age = 19.5 ± 1.2 years, height = 186.2 ± 5.7 cm, mass = 86.7 ± 7.0 kg; 48 right handed, 19 left handed). We measured peak ball velocity using a radar gun and shoulder and elbow kinetics of the throwing arm using 8 electronically synchronized, high-speed digital cameras. We placed 26 reflective markers on anatomical landmarks of each participant to track 3-dimensional coordinate data. The average data from the 3 highest-velocity fastballs thrown for strikes were used for data analysis. We calculated a Pearson correlation coefficient to determine the associations between ball velocity and peak elbow-valgus torque, shoulder-distraction force, and shoulder external-rotation torque (P < .05). A weak positive correlation was found between ball velocity and shoulder-distraction force (r = 0.257; 95% confidence interval [CI] = 0.02, 0.47; r(2) = 0.066; P = .018). However, no significant correlations were noted between ball velocity and elbow-valgus torque (r = 0.199; 95% CI = -0.043, 0.419; r(2) = 0.040; P = .053) or shoulder external-rotation torque (r = 0.097; 95% CI = -0.147, 0.329; r(2) = 0.009; P = .217). Although a weak positive correlation was present between ball velocity and shoulder-distraction force, no significant association was seen between ball velocity and elbow-valgus torque or shoulder external-rotation torque. Therefore, other factors, such as improper pitching mechanics, may contribute more to increases in joint kinetics than peak ball velocity.

  18. Correlation of Shoulder and Elbow Kinetics With Ball Velocity in Collegiate Baseball Pitchers

    PubMed Central

    Post, Eric G.; Laudner, Kevin G.; McLoda, Todd A.; Wong, Regan; Meister, Keith

    2015-01-01

    Context Throwing a baseball is a dynamic and violent act that places large magnitudes of stress on the shoulder and elbow. Specific injuries at the elbow and glenohumeral joints have been linked to several kinetic variables throughout the throwing motion. However, very little research has directly examined the relationship between these kinetic variables and ball velocity. Objective To examine the correlation of peak ball velocity with elbow-valgus torque, shoulder external-rotation torque, and shoulder-distraction force in a group of collegiate baseball pitchers. Design Cross-sectional study. Setting Motion-analysis laboratory. Patients or Other Participants Sixty-seven asymptomatic National Collegiate Athletic Association Division I baseball pitchers (age = 19.5 ± 1.2 years, height = 186.2 ± 5.7 cm, mass = 86.7 ± 7.0 kg; 48 right handed, 19 left handed). Main Outcome Measure(s) We measured peak ball velocity using a radar gun and shoulder and elbow kinetics of the throwing arm using 8 electronically synchronized, high-speed digital cameras. We placed 26 reflective markers on anatomical landmarks of each participant to track 3-dimensional coordinate data. The average data from the 3 highest-velocity fastballs thrown for strikes were used for data analysis. We calculated a Pearson correlation coefficient to determine the associations between ball velocity and peak elbow-valgus torque, shoulder-distraction force, and shoulder external-rotation torque (P < .05). Results A weak positive correlation was found between ball velocity and shoulder-distraction force (r = 0.257; 95% confidence interval [CI] = 0.02, 0.47; r2 = 0.066; P = .018). However, no significant correlations were noted between ball velocity and elbow-valgus torque (r = 0.199; 95% CI = −0.043, 0.419; r2 = 0.040; P = .053) or shoulder external-rotation torque (r = 0.097; 95% CI = −0.147, 0.329; r2 = 0.009; P = .217). Conclusions Although a weak positive correlation was present between ball velocity and shoulder-distraction force, no significant association was seen between ball velocity and elbow-valgus torque or shoulder external-rotation torque. Therefore, other factors, such as improper pitching mechanics, may contribute more to increases in joint kinetics than peak ball velocity. PMID:25756790

  19. On kinetics of a dynamically unbalanced rotator with sliding friction in supports

    NASA Astrophysics Data System (ADS)

    Chistyakov, Viktor V.

    2018-05-01

    The dynamics is analytically and numerically modelled for both free and forced rotations of a rigid body around the central but non-principal vertical axis Oz under action of dry friction forces in plain bearings and heel supports in combination with other dissipative and conservative axial torques. The inertia forces due to D'Alembert principle cause the supports' reactions and hence the decelerating friction torque depending on not only angular speed but acceleration too. This dependence makes the dynamical equations not resolved with regard to the senior derivative and ambiguous, and being thus resolved they have an irrational or singular right hand side. This irrationality/singularity results in their featured solutions or paradoxical absence of those in frames of absolutely rigid body approach. The kinetics obtained is analyzed and compared with the standard ones of rotation under action of conservative elastic and drag torques.

  20. Kinetic Shapes: Analysis, Verification, and Applications.

    PubMed

    Handz̆ić, Ismet; Reed, Kyle B

    2014-06-01

    A circular shape placed on an incline will roll; similarly, an irregularly shaped object, such as the Archimedean spiral, will roll on a flat surface when a force is applied to its axle. This rolling is dependent on the specific shape and the applied force (magnitude and location). In this paper, we derive formulas that define the behavior of irregular 2D and 3D shapes on a flat plane when a weight is applied to the shape's axle. These kinetic shape (KS) formulas also define and predict shapes that exert given ground reaction forces when a known weight is applied at the axle rotation point. Three 2D KS design examples are physically verified statically with good correlation to predicted values. Motion simulations of unrestrained 2D KS yielded expected results in shape dynamics and self-stabilization. We also put forth practical application ideas and research for 2D and 3D KS such as in robotics and gait rehabilitation.

  1. Probing the Mechanical Properties of Plasma von Willebrand Factor Using Atomic Force Microscopy

    NASA Astrophysics Data System (ADS)

    Wijeratne, Sitara; Botello, Eric; Frey, Eric; Kiang, Ching-Hwa; Dong, Jing-Fei; Yeh, Hui-Chun

    2010-03-01

    Single-molecule manipulation allows us to study the real time kinetics of many complex cellular processes. The mechanochemistry of different forms of von Willebrand factor (VWF) and their receptor-ligand binding kinetics can be unraveled by atomic force microscopy (AFM). Since plasma VWF can be activated upon shear, the structural and functional properties of VWF are critical in mediating thrombus formation become important. Here we characterized the mechanical resistance to domain unfolding of VWF to determine the conformational states of VWF. We found the shear induced conformational, hence mechanical property changes can be detected by the change in unfolding forces. The relaxation rate of such effect is much longed than expected. This supports the model of lateral association VWF under shear stress. Our results offer an insight in establishing strategies for regulating VWF adhesion activity, increasing our understanding of surface-induced thrombosis as mediated by VWF.

  2. Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy

    PubMed Central

    Ladoux, Benoit; Quivy, Jean-Pierre; Doyle, Patrick; Roure, Olivia du; Almouzni, Geneviève; Viovy, Jean-Louis

    2000-01-01

    Fluorescence videomicroscopy and scanning force microscopy were used to follow, in real time, chromatin assembly on individual DNA molecules immersed in cell-free systems competent for physiological chromatin assembly. Within a few seconds, molecules are already compacted into a form exhibiting strong similarities to native chromatin fibers. In these extracts, the compaction rate is more than 100 times faster than expected from standard biochemical assays. Our data provide definite information on the forces involved (a few piconewtons) and on the reaction path. DNA compaction as a function of time revealed unique features of the assembly reaction in these extracts. They imply a sequential process with at least three steps, involving DNA wrapping as the final event. An absolute and quantitative measure of the kinetic parameters of the early steps in chromatin assembly under physiological conditions could thus be obtained. PMID:11114182

  3. Jane Jacobs and ‘The Need for Aged Buildings’: Neighbourhood Historical Development Pace and Community Social Relations

    EPA Science Inventory

    Jacobs argued that grand planning schemes intending to redevelop large swaths of a city according to a central theoretical framework fail because planners do not understand that healthy cities are organic, spontaneous, messy, complex systems that result from evolutionary proces...

  4. New Orleans Nexus

    ERIC Educational Resources Information Center

    Bingler, Stephen

    2006-01-01

    The recovery and long-range redevelopment of New Orleans and the Gulf Coast region is a complex undertaking requiring simultaneous planning in a wide range of disciplines. There is a paramount need to create a planning infrastructure that will enhance collaboration and reduce duplication in all of the planning disciplines moving forward. To…

  5. 24 CFR 586.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false HUD's negotiations and consultations with the LRA. 586.25 Section 586.25 Housing and Urban Development Regulations Relating to Housing... COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 586.25 HUD's negotiations and...

  6. 24 CFR 586.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false HUD's negotiations and consultations with the LRA. 586.25 Section 586.25 Housing and Urban Development Regulations Relating to Housing... COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 586.25 HUD's negotiations and...

  7. 24 CFR 586.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true HUD's negotiations and consultations with the LRA. 586.25 Section 586.25 Housing and Urban Development Regulations Relating to Housing and... COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 586.25 HUD's negotiations and...

  8. 24 CFR 586.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false HUD's negotiations and consultations with the LRA. 586.25 Section 586.25 Housing and Urban Development Regulations Relating to Housing... COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 586.25 HUD's negotiations and...

  9. 24 CFR 586.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true HUD's negotiations and consultations with the LRA. 586.25 Section 586.25 Housing and Urban Development Regulations Relating to Housing and... COMMUNITY ASSISTANCE-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 586.25 HUD's negotiations and...

  10. 40 CFR 35.4190 - How does my group identify a qualified technical advisor?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... issues or public health issues as those issues relate to hazardous substance/toxic waste issues, as... public health issues must have received his or her public health or related training at accredited... hazardous or toxic waste problems, relocation, redevelopment or public health issues, and communicating...

  11. TECHNICAL APPROACHES TO CHARACTERIZING AND CLEANING UP IRON AND STEEL MILL SITES UNDER THE BROWNFIELDS INITIATIVE

    EPA Science Inventory

    This document provides brownfields planners with an overview of the technical methods that can be used to achieve successful site assessment and cleanup which are two key components of the brownfields redevelopment process. No two brownfields sites are identical and planners will...

  12. TECHNICAL APPROACHES TO CHARACTERIZING AND CLEANING UP AUTOMOTIVE REPAIR SITES UNDER THE BROWNFIELDS INITIATIVE

    EPA Science Inventory

    The document provides brownfields planners with an overview of the technical methods that can be used to achieve successful site assessment and cleanup which are two key components of the brownfields redevelopment process. No two brownfields sites are identical and planners will...

  13. 76 FR 17662 - Notice of Submission of Proposed Information Collection to OMB Brownfields Economic Development...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-30

    ... Proposed Information Collection to OMB Brownfields Economic Development Initiative (BEDI) Grant Application... subject proposal. BEDU we designed to help local governments redevelop brownfields, defined in the NOFA as... responses. This notice also lists the following information: Title of Proposal: Brownfields Economic...

  14. The Greening of a Brownfield: A Community-Based Learning Project in Economics

    ERIC Educational Resources Information Center

    Kaufman, Dennis A.; Cloutier, Norman R.

    2004-01-01

    This paper describes a community-based learning (CBL) project involving the economic redevelopment of two environmentally contaminated former industrial sites or "brownfields." The study was conducted over four semesters by several economics classes and involved assembling a database, measuring the impact of the brownfields on…

  15. 75 FR 69992 - Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) or Superfund...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-16

    ... can be located at http://www.epa.gov/brownfields . FOR FURTHER INFORMATION CONTACT: The U.S. EPA's Office of Solid Waste and Emergency Response, Office of Brownfields and Land Revitalization, (202) 566..., these response programs address the assessment, cleanup, and redevelopment of brownfields sites and...

  16. Attracting Private Investment to Contaminated Properties: The Value of Public Interventions

    ERIC Educational Resources Information Center

    Wernstedt, Kris; Meyer, Peter B.; Alberini, Anna

    2006-01-01

    We employ a mail survey of private developers that uses conjoint choice experiments and Likert-scaled attitudinal questions to examine preferences for policy instruments and incentives intended to encourage brownfield cleanup and redevelopment. Our analysis suggests that developers judge public hearing requirements at brownfield redevelopments…

  17. TECHNICAL APPROACHES TO CHARACTERIZING AND CLEANING UP METAL FINISHING SITES UNDER THE BROWNFIELDS INITIATIVE

    EPA Science Inventory

    The document provides brownfields planners with an overview of the technical methods that can be used to achieve successful site assessment and cleanup which are two key components of the brownfields redevelopment process. No two brownfields sites are identical and planners will...

  18. 32 CFR 174.10 - Consideration for economic development conveyances.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false Consideration for economic development... Property § 174.10 Consideration for economic development conveyances. (a) For conveyances made pursuant to... the date of the initial transfer of property shall be used to support economic redevelopment of, or...

  19. Reimagining Art & Ecology: There's No Place Like Home

    ERIC Educational Resources Information Center

    Krug, Don Herbert

    2005-01-01

    "From Good to Great--Stevenson Grows Wild" was an art and ecology project created by the teachers, students, and staff at Robert Louis Stevenson Elementary School in Grandview Heights, Ohio, in Spring 2002. This schoolyard habitat redevelopment was organized by the school librarian, three fourth-grade teachers, a graduate student, and…

  20. 32 CFR 174.6 - LRA and the redevelopment plan.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., including, but not limited to, representatives from those jurisdictions with zoning authority over the... practicable, complete the appropriate environmental documentation no later than 12 months after receipt of the... environmental analyses required under NEPA. (2) In the event there is no LRA recognized by DoD or if a...

  1. 32 CFR 174.6 - LRA and the redevelopment plan.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., including, but not limited to, representatives from those jurisdictions with zoning authority over the... practicable, complete the appropriate environmental documentation no later than 12 months after receipt of the... environmental analyses required under NEPA. (2) In the event there is no LRA recognized by DoD or if a...

  2. 76 FR 45772 - Proposed Foreign-Trade Zone-Brunswick, ME; Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-01

    ... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 49-2011] Proposed Foreign-Trade Zone--Brunswick, ME; Application An application has been submitted to the Foreign-Trade Zones Board (the Board) by the Midcoast Regional Redevelopment Authority to establish a general-purpose foreign-trade zone at a...

  3. The Familial Road to Healthy Societies: New and Converging Modes of Re-Development.

    ERIC Educational Resources Information Center

    Dyson, William A.

    This discussion focuses on problems facing developing and developed nations and describes social changes required to meet human needs in the economic condition of scarcity facing industrializing and postindustrial societies. Current problems of developed societies are emphasized. For example, traditional health care provision in western societies…

  4. Light Rail Transit in Hamilton: Health, Environmental and Economic Impact Analysis

    ERIC Educational Resources Information Center

    Topalovic, P.; Carter, J.; Topalovic, M.; Krantzberg, G.

    2012-01-01

    Hamilton's historical roots as an electric, industrial and transportation-oriented city provide it with a high potential for rapid transit, especially when combined with its growing population, developing economy, redeveloping downtown core and its plans for sustainable growth. This paper explores the health, environmental, social and economic…

  5. 78 FR 57208 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-17

    ... LEVEL: End of runway deicing program--phase 1, runway 34R. Taxiway S pavement reconstruction. Replace carousel 9 and oversized bag belt TU3. Terminal redevelopment program--design and associated technical... APPROVED FOR COLLECTION AND USE: PFC program administration. Design taxiways A, L and B. BRIEF DESCRIPTION...

  6. 32 CFR 174.10 - Consideration for economic development conveyances.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 1 2011-07-01 2011-07-01 false Consideration for economic development... Property § 174.10 Consideration for economic development conveyances. (a) For conveyances made pursuant to... the date of the initial transfer of property shall be used to support economic redevelopment of, or...

  7. 45 CFR 261.2 - What definitions apply to this part?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... in return for welfare, that provides an individual with an opportunity to acquire the general skills... work and that provides knowledge and skills essential to the full and adequate performance of the job... fields such as health, social service, environmental protection, education, urban and rural redevelopment...

  8. Is The Modern Lease a Contract or a Conveyance?--A Historical Inquiry

    ERIC Educational Resources Information Center

    Siegel, Stephen A.

    1975-01-01

    Argues that "contrary to conventional wisdom, current landlord-tenant law is modern, commercial, and already grounded in contract doctrine." Discusses the origins of the doctrines most frequently cited as illustrations of leasings property basis and redevelops the role of property law in adjudicating leasing disputes. (JT)

  9. 32 CFR 174.14 - Maintenance and repair.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and protect those facilities and items of equipment needed for reuse in an economical manner that... consultation with the LRA, will establish initial levels of maintenance and repair needed to aid redevelopment... levels of maintenance and repair and its duration. In no case will these initial levels of maintenance...

  10. 32 CFR 174.14 - Maintenance and repair.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... and protect those facilities and items of equipment needed for reuse in an economical manner that... consultation with the LRA, will establish initial levels of maintenance and repair needed to aid redevelopment... levels of maintenance and repair and its duration. In no case will these initial levels of maintenance...

  11. Mouse Driven Window Graphics for Network Teaching.

    ERIC Educational Resources Information Center

    Makinson, G. J.; And Others

    Computer enhanced teaching of computational mathematics on a network system driving graphics terminals is being redeveloped for a mouse-driven, high resolution, windowed environment of a UNIX work station. Preservation of the features of networked access by heterogeneous terminals is provided by the use of the X Window environment. A dmonstrator…

  12. 76 FR 12217 - Exempt Discretionary Program Grants (Section 5309) for Urban Circulator Systems

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    ... redevelopment of urban spaces into walkable mixed use, high density environments. FOR FURTHER INFORMATION... development. FTA's approval to advance the Urban Circulator projects through project development and grant... urban circulators shall be required to submit information that describes the impact of the urban...

  13. Revisiting Your Outdoor Environment: Reasons to Reshape, Enrich, Redevelop the Outdoor Space.

    ERIC Educational Resources Information Center

    Mauffette, Anne Gillain

    1998-01-01

    Provides suggestions for designing effective outdoor space. Focuses on advocating for space, designing spaces based on children's characteristics and preferences, integrating the outdoors in educational planning, including children in decision making and work, knowing about injury prevention, providing adult models who love the outdoors, and…

  14. 75 FR 38465 - Taking and Importing Marine Mammals; Taking Marine Mammals Incidental to the Port of Anchorage...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-02

    ..., from May 4 through November 18, 2009, trained graduate and undergraduate marine biology students... Importing Marine Mammals; Taking Marine Mammals Incidental to the Port of Anchorage Marine Terminal Redevelopment Project AGENCY: National Marine Fisheries Service, National Oceanic and Atmospheric Administration...

  15. Constructivism Pedagogy Drives Redevelopment of CAD Course: A Case Study

    ERIC Educational Resources Information Center

    Clemons, Stephanie A.

    2006-01-01

    Teacher and student roles continue to change as computers continue to influence the design curriculum (Kaplan, 1997). Educators and administrators continue to grapple with the best ways to teach, implement, and maintain current technology. Few technology teachers would argue that computers, software, and hardware found in technology laboratories…

  16. A color-blind pirate asks, “Do RRR’s make gray and green turn blue?”

    EPA Science Inventory

    Researchers and managers often do “R” projects when attempting to change conditions in natural and socioeconomic systems. Outputs from restoration, remediation, redevelopment, rehabilitation, reinvestment, and reuse, etc (hence, “R”) projects vary by application but have only a s...

  17. The Arcadia Commons Partnership: The Community College and Economic Redevelopment.

    ERIC Educational Resources Information Center

    Schlack, Marilyn

    The Arcadia Commons (AC), in Kalamazoo, Michigan, is a business-education park developed through the combined efforts of Kalamazoo Valley Community College (KVCC), area business and financial institutions, and the Kalamazoo Public Museum, who together formed Downtown Kalamazoo Incorporated (DKI). KVCC's involvement resulted from the need to find…

  18. COMPETITIVE BROWNFIELD REDEVELOPMENT - PROJECT MANAGEMENT AND MARKETING STRATEGIES (CD 2 OF 6)

    EPA Science Inventory

    The goal of this interactive CD is to inform urban planners and State and Federal Brownfield development personnel of new and innovative project management and marketing strategies related to brownfield activities. To order this CD by email: www.epa.gov/ncepi/ or phone 1-800-490-...

  19. Urban Property Taxation: II. Land and Location. Exchange Bibliography 480.

    ERIC Educational Resources Information Center

    White, Anthony G.

    This is one of three related bibliographies listing publications dealing with the broad topic of property taxation. This particular volume concerns some specialized fields of study, including locational theory, land use and taxation, property markets and valuation, housing, and urban renewal and redevelopment. Citations are listed alphabetically…

  20. Exploring synergies between transit investment and dense redevelopment: A scenario analysis in a rapidly urbanizing landscape

    EPA Science Inventory

    Like many urban areas around the world, Durham and Orange counties in North Carolina, USA are experiencing population growth and sprawl that is putting stress on the transportation system. Light rail and denser transit-oriented development are being considered as possible solutio...

  1. Three-Dimensional Lower Extremity Joint Loading in a Carved Ski and Snowboard Turn: A Pilot Study

    PubMed Central

    Müller, Erich

    2014-01-01

    A large number of injuries to the lower extremity occur in skiing and snowboarding. Due to the difficulty of collecting 3D kinematic and kinetic data with high accuracy, a possible relationship between injury statistic and joint loading has not been studied. Therefore, the purpose of the current study was to compare ankle and knee joint loading at the steering leg between carved ski and snowboard turns. Kinetic data were collected using mobile force plates mounted under the toe and heel part of the binding on skies or snowboard (KISTLER). Kinematic data were collected with five synchronized, panning, tilting, and zooming cameras. An extended version of the Yeadon model was applied to calculate inertial properties of the segments. Ankle and knee joint forces and moments were calculated using inverse dynamic analysis. Results showed higher forces along the longitudinal axis in skiing and similar forces for skiing and snowboarding in anterior-posterior and mediolateral direction. Joint moments were consistently greater during a snowboard turn, but more fluctuations were observed in skiing. Hence, when comparing joint loading between carved ski and snowboard turns, one should differentiate between forces and moments, including the direction of forces and moments and the turn phase. PMID:25317202

  2. The effect of workstation and task variables on forces applied during simulated meat cutting.

    PubMed

    McGorry, Raymond W; Dempsey, Patrick G; O'Brien, Niall V

    2004-12-01

    The purpose of the study was to investigate factors related to force and postural exposure during a simulated meat cutting task. The hypothesis was that workstation, tool and task variables would affect the dependent kinetic variables of gripping force, cutting moment and the dependent kinematic variables of elbow elevation and wrist angular displacement in the flexion/extension and radial/ulnar deviation planes. To evaluate this hypothesis a 3 x 3 x 2 x 2 x 2 (surface orientation by surface height by blade angle by cut complexity by work pace) within-subject factorial design was conducted with 12 participants. The results indicated that the variables can act and interact to modify the kinematics and kinetics of a cutting task. Participants used greater grip force and cutting moment when working at a pace based on productivity. The interactions of the work surface height and orientation indicated that the use of an adjustable workstation could minimize wrist deviation from neutral and improve shoulder posture during cutting operations. Angling the knife blade also interacted with workstation variables to improve wrist and upper extremity posture, but this benefit must be weighed against the potential for small increases in force exposure.

  3. The use of impact force as a scale parameter for the impact response of composite laminates

    NASA Technical Reports Server (NTRS)

    Jackson, Wade C.; Poe, C. C., Jr.

    1992-01-01

    The building block approach is currently used to design composite structures. With this approach, the data from coupon tests is scaled up to determine the design of a structure. Current standard impact tests and methods of relating test data to other structures are not generally understood and are often used improperly. A methodology is outlined for using impact force as a scale parameter for delamination damage for impacts of simple plates. Dynamic analyses were used to define ranges of plate parameters and impact parameters where quasi-static analyses are valid. These ranges include most low velocity impacts where the mass of the impacter is large and the size of the specimen is small. For large mass impacts of moderately thick (0.35 to 0.70 cm) laminates, the maximum extent of delamination damage increased with increasing impact force and decreasing specimen thickness. For large mass impact tests at a given kinetic energy, impact force and hence delamination size depends on specimen size, specimen thickness, boundary conditions, and indenter size and shape. If damage is reported in terms of impact force instead of kinetic energy, large mass test results can be applied directly to other plates of the same size.

  4. The use of impact force as a scale parameter for the impact response of composite laminates

    NASA Technical Reports Server (NTRS)

    Jackson, Wade C.; Poe, C. C., Jr.

    1992-01-01

    The building block approach is currently used to design composite structures. With this approach, the data from coupon tests are scaled up to determine the design of a structure. Current standard impact tests and methods of relating test data to other structures are not generally understood and are often used improperly. A methodology is outlined for using impact force as a scale parameter for delamination damage for impacts of simple plates. Dynamic analyses were used to define ranges of plate parameters and impact parameters where quasi-static analyses are valid. These ranges include most low-velocity impacts where the mass of the impacter is large, and the size of the specimen is small. For large-mass impacts of moderately thick (0.35-0.70 cm) laminates, the maximum extent of delamination damage increased with increasing impact force and decreasing specimen thickness. For large-mass impact tests at a given kinetic energy, impact force and hence delamination size depends on specimen size, specimen thickness, boundary conditions, and indenter size and shape. If damage is reported in terms of impact force instead of kinetic energy, large-mass test results can be applied directly to other plates of the same thickness.

  5. Impact of Temperature and Non-Gaussian Statistics on Electron Transfer in Donor-Bridge-Acceptor Molecules.

    PubMed

    Waskasi, Morteza M; Newton, Marshall D; Matyushov, Dmitry V

    2017-03-30

    A combination of experimental data and theoretical analysis provides evidence of a bell-shaped kinetics of electron transfer in the Arrhenius coordinates ln k vs 1/T. This kinetic law is a temperature analogue of the familiar Marcus bell-shaped dependence based on ln k vs the reaction free energy. These results were obtained for reactions of intramolecular charge shift between the donor and acceptor separated by a rigid spacer studied experimentally by Miller and co-workers. The non-Arrhenius kinetic law is a direct consequence of the solvent reorganization energy and reaction driving force changing approximately as hyperbolic functions with temperature. The reorganization energy decreases and the driving force increases when temperature is increased. The point of equality between them marks the maximum of the activationless reaction rate. Reaching the consistency between the kinetic and thermodynamic experimental data requires the non-Gaussian statistics of the donor-acceptor energy gap described by the Q-model of electron transfer. The theoretical formalism combines the vibrational envelope of quantum vibronic transitions with the Q-model describing the classical component of the Franck-Condon factor and a microscopic solvation model of the solvent reorganization energy and the reaction free energy.

  6. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Waskasi, Morteza M.; Newton, Marshall D.; Matyushov, Dmitry V.

    A combination of experimental data and theoretical analysis provides evidence of a bell-shaped kinetics of electron transfer in the Arrhenius coordinates ln k vs 1/T . This kinetic law is a temperature analog of the familiar Marcus bell-shaped dependence based on ln k vs the reaction free energy. These results were obtained for reactions of intramolecular charge shift between the donor and acceptor separated by a rigid spacer studied experimentally by Miller and co-workers. The non-Arrhenius kinetic law is a direct consequence of the solvent reorganization energy and reaction driving force changing approximately as hyperbolic functions with temperature. The reorganizationmore » energy decreases and the driving force increases when temperature is increased. The point of equality between them marks the maximum of the activationless reaction rate. Reaching the consistency between the kinetic and thermodynamic experimental data requires the non-Gaussian statistics of the donor-acceptor energy gap described by the Q-model of electron transfer. Furthermore, the theoretical formalism combines the vibrational envelope of quantum vibronic transitions with the Q-model describing the classical component of the Franck-Condon factor and a microscopic solvation model of the solvent reorganization energy and the reaction free energy.« less

  7. Kinematic and kinetic analysis of overhand, sidearm and underhand lacrosse shot techniques.

    PubMed

    Macaulay, Charles A J; Katz, Larry; Stergiou, Pro; Stefanyshyn, Darren; Tomaghelli, Luciano

    2017-12-01

    Lacrosse requires the coordinated performance of many complex skills. One of these skills is shooting on the opponents' net using one of three techniques: overhand, sidearm or underhand. The purpose of this study was to (i) determine which technique generated the highest ball velocity and greatest shot accuracy and (ii) identify kinematic and kinetic variables that contribute to a high velocity and high accuracy shot. Twelve elite male lacrosse players participated in this study. Kinematic data were sampled at 250 Hz, while two-dimensional force plates collected ground reaction force data (1000 Hz). Statistical analysis showed significantly greater ball velocity for the sidearm technique than overhand (P < 0.001) and underhand (P < 0.001) techniques. No statistical difference was found for shot accuracy (P > 0.05). Kinematic and kinetic variables were not significantly correlated to shot accuracy or velocity across all shot types; however, when analysed independently, the lead foot horizontal impulse showed a negative correlation with underhand ball velocity (P = 0.042). This study identifies the technique with the highest ball velocity, defines kinematic and kinetic predictors related to ball velocity and provides information to coaches and athletes concerned with improving lacrosse shot performance.

  8. Myoelectric activation and kinetics of different plyometric push-up exercises.

    PubMed

    García-Massó, Xavier; Colado, Juan C; González, Luis M; Salvá, Pau; Alves, Joao; Tella, Víctor; Triplett, N Travis

    2011-07-01

    The kinetic and myoelectric differences between 3 types of plyometric push-ups were investigated. Twenty-seven healthy, physically active men served as subjects and completed both familiarization and testing sessions. During these sessions, subjects performed 2 series of 3 plyometric push-up variations in a counterbalanced order according to the following techniques: Countermovement push-ups (CPUs) were push-ups performed with the maximum speed of movement; jump push-ups (JPUs) were similar to clapping push-ups; and fall push-ups (FPUs) required kneeling subjects to drop and then attempt to return to their initial position. Vertical ground reaction forces were determined by using a force plate. Myoelectric activity was recorded by means of electromyography. Impact force and impact rate of force development were significantly (p < 0.05) higher for FPUs than for JPUs. The maximum rate of force development was higher for CPUs (p < 0.05) than for JPUs, and the maximum force was higher for the CPUs than for the FPUs (p < 0.05). There were differences among exercises for the mean muscle activation of the pectoralis major (PM; p < 0.001), triceps brachii (p < 0.001), external oblique (p < 0.005) and anterior deltoid (p < 0.001), and in the maximum muscle activation of the PM (p < 0.001). Plyometric push-ups with countermovement achieved a higher maximum force and rate of force and did not cause impact forces. Thus, this type of push-up exercise may be regarded as the best for improving explosive force. The FPU exercise achieved higher levels of muscular activation in the agonist and synergist muscle groups, and greater impact forces and impact force development rates.

  9. Effects of dopamine replacement therapy on lower extremity kinetics and kinematics during a rapid force production task in persons with Parkinson disease.

    PubMed

    Foreman, K Bo; Singer, Madeline L; Addison, Odessa; Marcus, Robin L; LaStayo, Paul C; Dibble, Leland E

    2014-01-01

    Postural instability appears to be a dopamine resistance motor deficit in persons with Parkinson disease (PD); however, little is known about the effects of dopamine replacement on the relative biomechanical contributions of individual lower extremity joints during postural control tasks. To gain insight, we examined persons with PD using both clinical and laboratory measures. For a clinical measure of motor severity we utilized the Unified Parkinson Disease Rating Scale motor subsection during both OFF and ON medication conditions. For the laboratory measure we utilized data gathered during a rapid lower extremity force production task. Kinematic and kinetic variables at the hip, knee, and ankle were gathered during a counter movement jump during both OFF and ON medication conditions. Sixteen persons with PD with a median Hoehn and Yahr severity of 2.5 completed the study. Medication resulted in significant improvements of angular displacement for the hip, knee, and ankle. Furthermore, significant improvements were revealed only at the hip for peak net moments and average angular velocity compared to the OFF medication condition. These results suggest that dopamine replacement medication result in decreased clinical motor disease severity and have a greater influence on kinetics and kinematics proximally. This proximally focused improvement may be due to active recruitment of muscle force and reductions in passive restraint during lower extremity rapid force production. Copyright © 2013 Elsevier B.V. All rights reserved.

  10. Solvent Effects on Protein Folding/Unfolding

    NASA Astrophysics Data System (ADS)

    García, A. E.; Hillson, N.; Onuchic, J. N.

    Pressure effects on the hydrophobic potential of mean force led Hummer et al. to postulate a model for pressure denaturation of proteins in which denaturation occurs by means of water penetration into the protein interior, rather than by exposing the protein hydrophobic core to the solvent --- commonly used to describe temperature denaturation. We study the effects of pressure in protein folding/unfolding kinetics in an off-lattice minimalist model of a protein in which pressure effects have been incorporated by means of the pair-wise potential of mean force of hydrophobic groups in water. We show that pressure slows down the kinetics of folding by decreasing the reconfigurational diffusion coefficient and moves the location of the folding transition state.

  11. Atom-Pair Kinetics with Strong Electric-Dipole Interactions.

    PubMed

    Thaicharoen, N; Gonçalves, L F; Raithel, G

    2016-05-27

    Rydberg-atom ensembles are switched from a weakly to a strongly interacting regime via adiabatic transformation of the atoms from an approximately nonpolar into a highly dipolar quantum state. The resultant electric dipole-dipole forces are probed using a device akin to a field ion microscope. Ion imaging and pair-correlation analysis reveal the kinetics of the interacting atoms. Dumbbell-shaped pair-correlation images demonstrate the anisotropy of the binary dipolar force. The dipolar C_{3} coefficient, derived from the time dependence of the images, agrees with the value calculated from the permanent electric-dipole moment of the atoms. The results indicate many-body dynamics akin to disorder-induced heating in strongly coupled particle systems.

  12. On the origin of why static or breakloose friction is larger than kinetic friction, and how to reduce it: the role of aging, elasticity and sequential interfacial slip.

    PubMed

    Lorenz, B; Persson, B N J

    2012-06-06

    We discuss the origin of static friction and show how it can be reduced towards kinetic friction by the appropriate design of the sliding system. The basic idea is to use elastically soft solids and apply the external forces in such a way that different parts of the contacting interface start to slip at different times during the (tangential) loading process. In addition, the local slip must be large enough in order to result in a strong drop in the static friction force. We illustrate the theoretical predictions with the results of a simple model experiment.

  13. Dewetting kinetics of metallic liquid films: Competition between unbalanced Young's force and dissolutive reaction

    NASA Astrophysics Data System (ADS)

    Lu, Gui; Lin, Lin; Hui, Sheng; Wang, Shuo-Lin; Wang, Xiao-Dong; Lee, Duu-Jong

    2017-11-01

    Dewetting kinetics of Al and NiAl metallic liquid films on NiAl (1 0 0) substrates was studied using molecular dynamics simulations. A new dewetting-spreading transitional behavior was observed for high temperature dewetting. The dewetting-spreading transition comes from the competition between unbalanced Young's force and dissolutive reaction. Without dissolutive reaction, liquid films keep dewetting, but immediately turn into spreading when the dissolutive reaction involved. The dissolutive reaction depends on the initial Ni atom contents rather than the contact areas of dewetting films. The far-away-from saturated Ni content is the main mechanism which accelerates the wetting and reverses the dewetting process at high temperatures.

  14. Origami Arrays as Substrates for the Determination of Reaction Kinetics Using High-Speed Atomic Force Microscopy.

    PubMed

    Rahman, Masudur; Day, B Scott; Neff, David; Norton, Michael L

    2017-08-01

    DNA nanostructures (DN) are powerful platforms for the programmable assembly of nanomaterials. As applications for DN both as a structural material and as a support for functional biomolecular sensing systems develop, methods enabling the determination of reaction kinetics in real time become increasingly important. In this report, we present a study of the kinetics of streptavidin binding onto biotinylated DN constructs enabled by these planar structures. High-speed AFM was employed at a 2.5 frame/s rate to evaluate the kinetics and indicates that the binding fully saturates in less than 60 s. When the the data was fitted with an adsorption-limited kinetic model, a forward rate constant of 5.03 × 10 5 s -1 was found.

  15. Accurate prediction of vaccine stability under real storage conditions and during temperature excursions.

    PubMed

    Clénet, Didier

    2018-04-01

    Due to their thermosensitivity, most vaccines must be kept refrigerated from production to use. To successfully carry out global immunization programs, ensuring the stability of vaccines is crucial. In this context, two important issues are critical, namely: (i) predicting vaccine stability and (ii) preventing product damage due to excessive temperature excursions outside of the recommended storage conditions (cold chain break). We applied a combination of advanced kinetics and statistical analyses on vaccine forced degradation data to accurately describe the loss of antigenicity for a multivalent freeze-dried inactivated virus vaccine containing three variants. The screening of large amounts of kinetic models combined with a statistical model selection approach resulted in the identification of two-step kinetic models. Predictions based on kinetic analysis and experimental stability data were in agreement, with approximately five percentage points difference from real values for long-term stability storage conditions, after excursions of temperature and during experimental shipments of freeze-dried products. Results showed that modeling a few months of forced degradation can be used to predict various time and temperature profiles endured by vaccines, i.e. long-term stability, short time excursions outside the labeled storage conditions or shipments at ambient temperature, with high accuracy. Pharmaceutical applications of the presented kinetics-based approach are discussed. Copyright © 2018 The Author. Published by Elsevier B.V. All rights reserved.

  16. A new atomic force microscope force ramp technique using digital force feedback control reveals mechanically weak protein unfolding events.

    PubMed

    Kawakami, M; Smith, D A

    2008-12-10

    We have developed a new force ramp modification of the atomic force microscope (AFM) which can control multiple unfolding events of a multi-modular protein using software-based digital force feedback control. With this feedback the force loading rate can be kept constant regardless the length of soft elastic linkage or number of unfolded polypeptide domains. An unfolding event is detected as a sudden drop in force, immediately after which the feedback control reduces the applied force to a low value of a few pN by lowering the force set point. Hence the remaining folded domains can relax and the subsequent force ramp is applied to relaxed protein domains identically in each case. We have applied this technique to determine the kinetic parameters x(u), which is the distance between the native state and transition state, and α(0), which is the unfolding rate constant at zero force, for the mechanical unfolding of a pentamer of I27 domains of titin. In each force ramp the unfolding probability depends on the number of folded domains remaining in the system and we had to take account of this effect in the analysis of unfolding force data. We obtained values of x(u) and α(0) to be 0.28 nm and 1.02 × 10(-3) s(-1), which are in good agreement with those obtained from conventional constant velocity experiments. This method reveals unfolding data at low forces that are not seen in constant velocity experiments and corrects for the change in stiffness that occurs with most mechanical systems throughout the unfolding process to allow constant force ramp experiments to be carried out. In addition, a mechanically weak structure was detected, which formed from the fully extended polypeptide chain during a force quench. This indicates that the new technique will allow studies of the folding kinetics of previously hidden, mechanically weak species.

  17. Upper limb joint kinetics of three sitting pivot wheelchair transfer techniques in individuals with spinal cord injury.

    PubMed

    Kankipati, Padmaja; Boninger, Michael L; Gagnon, Dany; Cooper, Rory A; Koontz, Alicia M

    2015-07-01

    Repeated measures design. This study compared the upper extremity (UE) joint kinetics between three transfer techniques. Research laboratory. Twenty individuals with spinal cord injury performed three transfer techniques from their wheelchair to a level tub bench. Two of the techniques involved a head-hips method with leading hand position close (HH-I) and far (HH-A) from the body, and the third technique with the trunk upright (TU) and hand far from body. Motion analysis equipment recorded upper body movements and force sensors recorded their hand and feet reaction forces during the transfers. Several significant differences were found between HH-A and HH-I and TU and HH-I transfers indicating that hand placement was a key factor influencing the UE joint kinetics. Peak resultant hand, elbow, and shoulder joint forces were significantly higher for the HH-A and TU techniques at the trailing arm (P < 0.036) and lower at the leading arm (P < 0.021), compared to the HH-I technique. Always trailing with the same arm if using HH-A or TU could predispose that arm to overuse related pain and injuries. Technique training should focus on initial hand placement close to the body followed by the amount of trunk flexion needed to facilitate movement.

  18. Ring current Atmosphere interactions Model with Self-Consistent Magnetic field

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jordanova, Vania; Jeffery, Christopher; Welling, Daniel

    The Ring current Atmosphere interactions Model with Self-Consistent magnetic field (B) is a unique code that combines a kinetic model of ring current plasma with a three dimensional force-balanced model of the terrestrial magnetic field. The kinetic portion, RAM, solves the kinetic equation to yield the bounce-averaged distribution function as a function of azimuth, radial distance, energy and pitch angle for three ion species (H+, He+, and O+) and, optionally, electrons. The domain is a circle in the Solar-Magnetic (SM) equatorial plane with a radial span of 2 to 6.5 RE. It has an energy range of approximately 100 eVmore » to 500 KeV. The 3-D force balanced magnetic field model, SCB, balances the JxB force with the divergence of the general pressure tensor to calculate the magnetic field configuration within its domain. The domain ranges from near the Earth’s surface, where the field is assumed dipolar, to the shell created by field lines passing through the SM equatorial plane at a radial distance of 6.5 RE. The two codes work in tandem, with RAM providing anisotropic pressure to SCB and SCB returning the self-consistent magnetic field through which RAM plasma is advected.« less

  19. Weight-bearing asymmetries during Sit-To-Stand in patients with mild-to-moderate hip osteoarthritis.

    PubMed

    Eitzen, Ingrid; Fernandes, Linda; Nordsletten, Lars; Snyder-Mackler, Lynn; Risberg, May Arna

    2014-02-01

    The Sit-To-Stand (STS) transition is a mechanically demanding task that may pose particular challenges for individuals with lower limb osteoarthritis (OA). Biomechanical features of STS have been investigated in patients with OA, but not in patients with early stage hip OA. The purpose of this study was to explore inter-limb weight-bearing asymmetries (WBA) and selected kinematic and kinetic variables during STS in patients with mild-to-moderate hip OA compared with healthy controls. Twenty-one hip OA patients and 23 controls were included in the study. Sagittal and frontal plane kinematic and kinetic data were collected using an eight-camera motion analysis system synchronized with two force plates embedded in the floor. There were no distinctive biomechanical alterations in sagittal or frontal plane kinematics or kinetics, movement time, or time to reach peak ground reaction force (GRF) in hip OA patients compared with controls. However, the hip OA patients revealed a distinct pattern of WBA compared with the controls, in unloading their involved limb by 18.4% at peak GRF. These findings indicate that patients with early stage hip OA are not yet forced into a stereotypical movement strategy for STS; however, the observed pattern of WBA requires clinical attention. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Characterization of fine motor development: dynamic analysis of children's drawing movements.

    PubMed

    Lin, Qiushi; Luo, Jianfei; Wu, Zhongcheng; Shen, Fei; Sun, Zengwu

    2015-04-01

    In this study, we investigated children's fine motor development by analyzing drawing trajectories, kinematics and kinetics. Straight lines drawing task and circles drawing task were performed by using a force sensitive tablet. Forty right-handed and Chinese mother-tongue students aged 6-12, attending classes from grade 1 to 5, were engaged in the experiment. Three spatial parameters, namely cumulative trace length, vector length of straight line and vertical diameter of circle were determined. Drawing duration, mean drawing velocity, and number of peaks in stroke velocity profile (NPV) were derived as kinematic parameters. Besides mean normal force, two kinetic indices were proposed: normalized force angle regulation (NFR) and variation of fine motor control (VFC) for circles drawing task. The maturation and automation of fine motor ability were reflected by increased drawing velocity, reduced drawing duration, NPV and NFR, with decreased VFC in circles drawing task. Grade and task main effects as well as significant correlations between age and parameters suggest that factors such as schooling, age and task should be considered in the assessment of fine motor skills. Compared with kinematic parameters, findings of NFR and VFC revealed that kinetics is another important perspective in the analysis of fine motor movement. Copyright © 2014 Elsevier B.V. All rights reserved.

  1. The Influence of Minimalist and Maximalist Footwear on Patellofemoral Kinetics During Running.

    PubMed

    Sinclair, Jonathan; Richards, Jim; Selfe, James; Fau-Goodwin, James; Shore, Hannah

    2016-08-01

    The current study aimed to comparatively examine the effects of minimalist, maximalist, and conventional footwear on the loads experienced by the patellofemoral joint during running. Twenty male participants ran over a force platform at 4.0 m×s-1. Lower limb kinematics were collected using an 8-camera motion capture system allowing patellofemoral kinetics to be quantified using a musculoskeletal modeling approach. Differences in patellofemoral kinetic parameters were examined using one-way repeated-measures ANOVA. The results showed the peak patellofemoral force and pressure were significantly larger in conventional (4.70 ± 0.91 BW, 13.34 ± 2.43 MPa) and maximalist (4.74 ± 0.88 BW, 13.59 ± 2.63 MPa) compared with minimalist footwear (3.87 ± 1.00 BW, 11.59 ± 2.63 MPa). It was also revealed that patellofemoral force per mile was significantly larger in conventional (246.81 ± 53.21 BW) and maximalist (251.94 ± 59.17 BW) as compared with minimalist (227.77 ± 58.60 BW) footwear. As excessive loading of the patellofemoral joint has been associated with the etiology of patellofemoral pain symptoms, the current investigation indicates that minimalist footwear may be able reduce runners' susceptibility to patellofemoral disorders.

  2. Kinetics of Some Metal Atom and Metal Fluoride Oxidation Reactions Relevant to Air Force Technology Development

    DTIC Science & Technology

    1981-03-01

    Products and Chemicals , Inc ., supplied a complete analysis with each cylinder. Initial measurements with the original batch (cylinder 1) were considered...NF 3] in this limited tempera- ture range. The NFs used in these experiments was made available by Kelly Air Force Base. The manufacturer, Air

  3. Spatial distribution of mechanical forces and ionic flux in electro-kinetic instability near a permselective membrane

    NASA Astrophysics Data System (ADS)

    Magnico, Pierre

    2018-01-01

    This paper is devoted to the numerical investigation of electro-kinetic instability in a polarization layer next to a cation-exchange membrane. An analysis of some properties of the electro-kinetic instability is followed by a detailed description of the fluid flow structure and of the spatial distribution of the ionic flux. In this aim, the Stokes-Poisson-Nernst-Planck equation set is solved until the Debye length scale. The results show that the potential threshold of the marginal instability and the current density depend on the logarithm of the concentration at the membrane surface. The size of the stable vortices seems to be an increasing function of the potential drop. The fluid motion is induced by the electric force along the maximum concentration in the extended space charge (ESC) region and by the pressure force in the region around the inner edge of the ESC layer. Two spots of kinetic energy are located in the ESC region and between the vortices. The cationic motion, controlled by the electric field and the convection, presents counter-rotating vortices in the stagnation zone located in the fluid ejection region. The anion transport is also characterized by two independent layers which contain counter-rotating vortices. The first one is in contact with the stationary reservoir. In the second layer against the membrane, the convection, and the electric field control, the transversal motion, the Fickian diffusion, and the convection are dominant in the longitudinal direction. Finally, the longitudinal disequilibrium of potential and pressure along the membrane is analyzed.

  4. Hip rotation angle is associated with frontal plane knee joint mechanics during running.

    PubMed

    Sakaguchi, Masanori; Shimizu, Norifumi; Yanai, Toshimasa; Stefanyshyn, Darren J; Kawakami, Yasuo

    2015-02-01

    Inability to control lower extremity segments in the frontal and transverse planes resulting in large knee abduction angle and increased internal knee abduction impulse has been associated with patellofemoral pain (PFP). However, the influence of hip rotation angles on frontal plane knee joint kinematics and kinetics remains unclear. The purpose of this study was to explore how hip rotation angles are related to frontal plane knee joint kinematics and kinetics during running. Seventy runners participated in this study. Three-dimensional marker positions and ground reaction forces were recorded with an 8-camera motion analysis system and a force plate while subjects ran along a 25-m runway at a speed of 4m/s. Knee abduction, hip rotation and toe-out angles, frontal plane lever arm at the knee, internal knee abduction moment and impulse, ground reaction forces and the medio-lateral distance from the ankle joint center to the center of pressure (AJC-CoP) were quantified. The findings of this study indicate that greater hip external rotation angles were associated with greater toe-out angles, longer AJC-CoP distances, smaller internal knee abduction impulses with shorter frontal plane lever arms and greater knee abduction angles. Thus, there appears to exist a conflict between kinematic and kinetic risk factors of PFP, and hip external rotation angle may be a key factor to control frontal plane knee joint kinematics and kinetics. These results may help provide an appropriate manipulation and/or intervention on running style to reduce the risk of PFP. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Kinetic Analysis of Horizontal Plyometric Exercise Intensity.

    PubMed

    Kossow, Andrew J; Ebben, William P

    2018-05-01

    Kossow, AJ, DeChiara, TG, Neahous, SM, and Ebben, WP. Kinetic analysis of horizontal plyometric exercise intensity. J Strength Cond Res 32(5): 1222-1229, 2018-Plyometric exercises are frequently performed as part of a strength and conditioning program. Most studies assessed the kinetics of plyometric exercises primarily performed in the vertical plane. The purpose of this study was to evaluate the multiplanar kinetic characteristics of a variety of plyometric exercises, which have a significant horizontal component. This study also sought to assess sex differences in the intensity progression of these exercises. Ten men and 10 women served as subjects. The subjects performed a variety of plyometric exercises including the double-leg hop, standing long jump, single-leg standing long jump, bounding, skipping, power skipping, cone hops, and 45.72-cm hurdle hops. Subjects also performed the countermovement jump for comparison. All plyometric exercises were evaluated using a force platform. Dependent variables included the landing rate of force development and landing ground reaction forces for each exercise in the vertical, frontal, and sagittal planes. A 2-way mixed analysis of variance with repeated-measures for plyometric exercise type demonstrated main effects for exercise type for all dependent variables (p ≤ 0.001). There was no significant interaction between plyometric exercise type and sex for any of the variable assessed. Bonferroni-adjusted pairwise comparisons identified a number of differences between the plyometric exercises for the dependent variables assessed (p ≤ 0.05). These findings should be used to guide practitioners in the progression of plyometric exercise intensity, and thus program design, for those who require significant horizontal power in their sport.

  6. Ambulatory measurement of ankle kinetics for clinical applications.

    PubMed

    Rouhani, H; Favre, J; Crevoisier, X; Aminian, K

    2011-10-13

    This study aimed to design and validate the measurement of ankle kinetics (force, moment, and power) during consecutive gait cycles and in the field using an ambulatory system. An ambulatory system consisting of plantar pressure insole and inertial sensors (3D gyroscopes and 3D accelerometers) on foot and shank was used. To test this system, 12 patients and 10 healthy elderly subjects wore shoes embedding this system and walked many times across a gait lab including a force-plate surrounded by seven cameras considered as the reference system. Then, the participants walked two 50-meter trials where only the ambulatory system was used. Ankle force components and sagittal moment of ankle measured by ambulatory system showed correlation coefficient (R) and normalized RMS error (NRMSE) of more than 0.94 and less than 13% in comparison with the references system for both patients and healthy subjects. Transverse moment of ankle and ankle power showed R>0.85 and NRMSE<23%. These parameters also showed high repeatability (CMC>0.7). In contrast, the ankle coronal moment of ankle demonstrated high error and lower repeatability. Except for ankle coronal moment, the kinetic features obtained by the ambulatory system could distinguish the patients with ankle osteoarthritis from healthy subjects when measured in 50-meter trials. The proposed ambulatory system can be easily accessible in most clinics and could assess main ankle kinetics quantities with acceptable error and repeatability for clinical evaluations. This system is therefore suggested for field measurement in clinical applications. Copyright © 2011 Elsevier Ltd. All rights reserved.

  7. Force illusions and drifts observed during muscle vibration.

    PubMed

    Reschechtko, Sasha; Cuadra, Cristian; Latash, Mark L

    2018-01-01

    We explored predictions of a scheme that views position and force perception as a result of measuring proprioceptive signals within a reference frame set by ongoing efferent process. In particular, this hypothesis predicts force illusions caused by muscle vibration and mediated via changes in both afferent and efferent components of kinesthesia. Healthy subjects performed accurate steady force production tasks by pressing with the four fingers of one hand (the task hand) on individual force sensors with and without visual feedback. At various times during the trials, subjects matched the perceived force using the other hand. High-frequency vibration was applied to one or both of the forearms (over the hand and finger extensors). Without visual feedback, subjects showed a drop in the task hand force, which was significantly smaller under the vibration of that forearm. Force production by the matching hand was consistently higher than that of the task hand. Vibrating one of the forearms affected the matching hand in a manner consistent with the perception of higher magnitude of force produced by the vibrated hand. The findings were consistent between the dominant and nondominant hands. The effects of vibration on both force drift and force mismatching suggest that vibration led to shifts in both signals from proprioceptors and the efferent component of perception, the referent coordinate and/or coactivation command. The observations fit the hypothesis on combined perception of kinematic-kinetic variables with little specificity of different groups of peripheral receptors that all contribute to perception of forces and coordinates. NEW & NOTEWORTHY We show that vibration of hand/finger extensors produces consistent errors in finger force perception. Without visual feedback, finger force drifted to lower values without a drift in the matching force produced by the other hand; hand extensor vibration led to smaller finger force drift. The findings fit the scheme with combined perception of kinematic-kinetic variables and suggest that vibration leads to consistent shifts of the referent coordinate and, possibly, of coactivation command to the effector.

  8. Stiffness Effects in Rocker-Soled Shoes: Biomechanical Implications

    PubMed Central

    Su, Pei-Fang; Chung, Chia-Hua; Hsia, Chi-Chun; Chang, Chih-Han

    2017-01-01

    Rocker-soled shoes provide a way to reduce the possible concentration of stress, as well as change movement patterns, during gait. This study attempts to examine how plantar force and spatio-temporal variables are affected by two rocker designs, one with softer and one with denser sole materials, by comparing them with the barefoot condition and with flat-soled shoes. Eleven subjects’ gait parameters during walking and jogging were recorded. Our results showed that compared with barefoot walking, plantar forces were higher for flat shoes while lower for both types of rocker shoes, the softer-material rocker being the lowest. The plantar force of flat shoes is greater than the vertical ground reaction force, while that of both rocker shoes is much less, 13.87–30.55% body weight. However, as locomotion speed increased to jogging, for all shoe types, except at the second peak plantar force of the denser sole material rocker shoes, plantar forces were greater than for bare feet. More interestingly, because the transmission of force was faster while jogging, greater plantar force was seen in the rocker-soled shoes with softer material than with denser material; results for higher-speed shock absorption in rocker-soled shoes with softer material were thus not as good. In general, the rolling phenomena along the bottom surface of the rocker shoes, as well as an increase in the duration of simultaneous curve rolling and ankle rotation, could contribute to the reduction of plantar force for both rocker designs. The possible mechanism is the conversion of vertical kinetic energy into rotational kinetic energy. To conclude, since plantar force is related to foot-ground interface and deceleration methods, rocker-design shoes could achieve desired plantar force reduction through certain rolling phenomena, shoe-sole stiffness levels, and locomotion speeds. PMID:28046009

  9. Stiffness Effects in Rocker-Soled Shoes: Biomechanical Implications.

    PubMed

    Lin, Shih-Yun; Su, Pei-Fang; Chung, Chia-Hua; Hsia, Chi-Chun; Chang, Chih-Han

    2017-01-01

    Rocker-soled shoes provide a way to reduce the possible concentration of stress, as well as change movement patterns, during gait. This study attempts to examine how plantar force and spatio-temporal variables are affected by two rocker designs, one with softer and one with denser sole materials, by comparing them with the barefoot condition and with flat-soled shoes. Eleven subjects' gait parameters during walking and jogging were recorded. Our results showed that compared with barefoot walking, plantar forces were higher for flat shoes while lower for both types of rocker shoes, the softer-material rocker being the lowest. The plantar force of flat shoes is greater than the vertical ground reaction force, while that of both rocker shoes is much less, 13.87-30.55% body weight. However, as locomotion speed increased to jogging, for all shoe types, except at the second peak plantar force of the denser sole material rocker shoes, plantar forces were greater than for bare feet. More interestingly, because the transmission of force was faster while jogging, greater plantar force was seen in the rocker-soled shoes with softer material than with denser material; results for higher-speed shock absorption in rocker-soled shoes with softer material were thus not as good. In general, the rolling phenomena along the bottom surface of the rocker shoes, as well as an increase in the duration of simultaneous curve rolling and ankle rotation, could contribute to the reduction of plantar force for both rocker designs. The possible mechanism is the conversion of vertical kinetic energy into rotational kinetic energy. To conclude, since plantar force is related to foot-ground interface and deceleration methods, rocker-design shoes could achieve desired plantar force reduction through certain rolling phenomena, shoe-sole stiffness levels, and locomotion speeds.

  10. Hand-rim forces and gross mechanical efficiency in asynchronous and synchronous wheelchair propulsion: a comparison.

    PubMed

    Lenton, J P; van der Woude, L; Fowler, N; Nicholson, G; Tolfrey, K; Goosey-Tolfrey, V

    2014-03-01

    To compare the force application characteristics at various push frequencies of asynchronous (ASY) and synchronous (SYN) hand-rim propulsion, 8 able-bodied participants performed a separate sub-maximal exercise test on a wheelchair roller ergometer for each propulsion mode. Each test consisted of a series of 5, 4-min exercise blocks at 1.8 m · s-1 - initially at their freely chosen frequency (FCF), followed by four counter-balanced trials at 60, 80, 120 and 140% FCF. Kinetic data was obtained using a SMARTWheel, measuring forces and moments. The gross efficiency (GE) was determined as the ratio of external work done and the total energy expended. The ASY propulsion produced higher force measures for FRES, FTAN, rate of force development & FEF (P<0.05), while there was no difference in GE values (P=0.518). In pair-matched push frequencies (ASY80:SYN60, ASY100:SYN80, ASY120:SYN100 and ASY140:SYN120), ASY propulsion forces remained significantly higher (FRES, FTAN, rate of force development & FEF P<0.05), and there was no significant effect on GE (P=0.456). Both ASY and SYN propulsion demonstrate similar trends: changes in push frequency are accompanied by changes in absolute force even without changes in the gross pattern/trend of force application, FEF or GE. Matched push frequencies continue to produce significant differences in force measures but not GE. This suggests ASY propulsion is the predominant factor in force application differences. The ASY would appear to offer a kinetic disadvantage to SYN propulsion and no physiological advantage under current testing conditions. © Georg Thieme Verlag KG Stuttgart · New York.

  11. Accuracy and precision of loadsol® insole force-sensors for the quantification of ground reaction force-based biomechanical running parameters.

    PubMed

    Seiberl, Wolfgang; Jensen, Elisabeth; Merker, Josephine; Leitel, Marco; Schwirtz, Ansgar

    2018-05-29

    Force plates represent the "gold standard" in measuring running kinetics to predict performance or to identify the sources of running-related injuries. As these measurements are generally limited to laboratory analyses, wireless high-quality sensors for measuring in the field are needed. This work analysed the accuracy and precision of a new wireless insole forcesensor for quantifying running-related kinetic parameters. Vertical ground reaction force (GRF) was simultaneously measured with pit-mounted force plates (1 kHz) and loadsol ® sensors (100 Hz) under unshod forefoot and rearfoot running-step conditions. GRF data collections were repeated four times, each separated by 30 min treadmill running, to test influence of extended use. A repeated-measures ANOVA was used to identify differences between measurement devices. Additionally, mean bias and Bland-Altman limits of agreement (LoA) were calculated. We found a significant difference (p < .05) in ground contact time, peak force, and force rate, while there was no difference in parameters impulse, time to peak, and negative force rate. There was no influence of time point of measurement. The mean bias of ground contact time, impulse, peak force, and time to peak ranged between 0.6% and 3.4%, demonstrating high accuracy of loadsol ® devices for these parameters. For these same parameters, the LoA analysis showed that 95% of all measurement differences between insole and force plate measurements were less than 12%, demonstrating high precision of the sensors. However, highly dynamic behaviour of GRF, such as force rate, is not yet sufficiently resolved by the insole devices, which is likely explained by the low sampling rate.

  12. Aerodynamic and Kinetic Processes in Flames

    DTIC Science & Technology

    1989-09-15

    Julian Tishkoff Air Force Office of Scientific Research Boiling Air Force Base Submitted by Professor Chung K. Law Department of Mechanical and...87ER13274 and the Air MILLt.ER, J. A.: Twettt-iFirst Svittpitsitli klitel.- Force Office of Scientific Research under Grant No. tational) on Comthustiont, p...thle individual effects is expected. For C\\.- of the majur heat release reactions are inulch shorter ample. whIIile Tl/, dlepenlds omi l\\ on q/. thle pit

  13. Three-dimensional stochastic model of actin–myosin binding in the sarcomere lattice

    PubMed Central

    Kayser-Herold, Oliver; Stojanovic, Boban; Nedic, Djordje; Irving, Thomas C.; Geeves, Michael A.

    2016-01-01

    The effect of molecule tethering in three-dimensional (3-D) space on bimolecular binding kinetics is rarely addressed and only occasionally incorporated into models of cell motility. The simplest system that can quantitatively determine this effect is the 3-D sarcomere lattice of the striated muscle, where tethered myosin in thick filaments can only bind to a relatively small number of available sites on the actin filament, positioned within a limited range of thermal movement of the myosin head. Here we implement spatially explicit actomyosin interactions into the multiscale Monte Carlo platform MUSICO, specifically defining how geometrical constraints on tethered myosins can modulate state transition rates in the actomyosin cycle. The simulations provide the distribution of myosin bound to sites on actin, ensure conservation of the number of interacting myosins and actin monomers, and most importantly, the departure in behavior of tethered myosin molecules from unconstrained myosin interactions with actin. In addition, MUSICO determines the number of cross-bridges in each actomyosin cycle state, the force and number of attached cross-bridges per myosin filament, the range of cross-bridge forces and accounts for energy consumption. At the macroscopic scale, MUSICO simulations show large differences in predicted force-velocity curves and in the response during early force recovery phase after a step change in length comparing to the two simplest mass action kinetic models. The origin of these differences is rooted in the different fluxes of myosin binding and corresponding instantaneous cross-bridge distributions and quantitatively reflects a major flaw of the mathematical description in all mass action kinetic models. Consequently, this new approach shows that accurate recapitulation of experimental data requires significantly different binding rates, number of actomyosin states, and cross-bridge elasticity than typically used in mass action kinetic models to correctly describe the biochemical reactions of tethered molecules and their interaction energetics. PMID:27864330

  14. Tunneling Kinetics and Nonadiabatic Proton-Coupled Electron Transfer in Proteins: The Effect of Electric Fields and Anharmonic Donor-Acceptor Interactions.

    PubMed

    Salna, Bridget; Benabbas, Abdelkrim; Russo, Douglas; Champion, Paul M

    2017-07-20

    A proper description of proton donor-acceptor (D-A) distance fluctuations is crucial for understanding tunneling in proton-coupled electron transport (PCET). The typical harmonic approximation for the D-A potential results in a Gaussian probability distribution, which does not appropriately reflect the electronic repulsion forces that increase the energetic cost of sampling shorter D-A distances. Because these shorter distances are the primary channel for thermally activated tunneling, the analysis of tunneling kinetics depends sensitively on the inherently anharmonic nature of the D-A interaction. Thus, we have used quantum chemical calculations to account for the D-A interaction and developed an improved model for the analysis of experimental tunneling kinetics. Strong internal electric fields are also considered and found to contribute significantly to the compressive forces when the D-A distance distribution is positioned below the van der Waals contact distance. This model is applied to recent experiments on the wild type (WT) and a double mutant (DM) of soybean lipoxygenase-1 (SLO). The compressive force necessary to prepare the tunneling-active distribution in WT SLO is found to fall in the ∼ nN range, which greatly exceeds the measured values of molecular motor and protein unfolding forces. This indicates that ∼60-100 MV/cm electric fields, aligned along the D-A bond axis, must be generated by an enzyme conformational interconversion that facilitates the PCET tunneling reaction. Based on the absolute value of the measured tunneling rate, and using previously calculated values of the electronic matrix element, the population of this tunneling-active conformation is found to lie in the range 10 -5 -10 -7 , indicating this is a rare structural fluctuation that falls well below the detection threshold of recent ENDOR experiments. Additional analysis of the DM tunneling kinetics leads to a proposal that a disordered (high entropy) conformation could be tunneling-active due to its broad range of sampled D-A distances.

  15. Three-dimensional stochastic model of actin–myosin binding in the sarcomere lattice

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mijailovich, Srboljub M.; Kayser-Herold, Oliver; Stojanovic, Boban

    2016-11-18

    The effect of molecule tethering in three-dimensional (3-D) space on bimolecular binding kinetics is rarely addressed and only occasionally incorporated into models of cell motility. The simplest system that can quantitatively determine this effect is the 3-D sarcomere lattice of the striated muscle, where tethered myosin in thick filaments can only bind to a relatively small number of available sites on the actin filament, positioned within a limited range of thermal movement of the myosin head. Here we implement spatially explicit actomyosin interactions into the multiscale Monte Carlo platform MUSICO, specifically defining how geometrical constraints on tethered myosins can modulatemore » state transition rates in the actomyosin cycle. The simulations provide the distribution of myosin bound to sites on actin, ensure conservation of the number of interacting myosins and actin monomers, and most importantly, the departure in behavior of tethered myosin molecules from unconstrained myosin interactions with actin. In addition, MUSICO determines the number of cross-bridges in each actomyosin cycle state, the force and number of attached cross-bridges per myosin filament, the range of cross-bridge forces and accounts for energy consumption. At the macroscopic scale, MUSICO simulations show large differences in predicted force-velocity curves and in the response during early force recovery phase after a step change in length comparing to the two simplest mass action kinetic models. The origin of these differences is rooted in the different fluxes of myosin binding and corresponding instantaneous cross-bridge distributions and quantitatively reflects a major flaw of the mathematical description in all mass action kinetic models. Consequently, this new approach shows that accurate recapitulation of experimental data requires significantly different binding rates, number of actomyosin states, and cross-bridge elasticity than typically used in mass action kinetic models to correctly describe the biochemical reactions of tethered molecules and their interaction energetics.« less

  16. The Magnetorotational Instability in a Collisionless Plasma

    NASA Astrophysics Data System (ADS)

    Quataert, Eliot; Dorland, William; Hammett, Gregory W.

    2002-09-01

    We consider the linear axisymmetric stability of a differentially rotating collisionless plasma in the presence of a weak magnetic field; we restrict our analysis to wavelengths much larger than the proton Larmor radius. This is the kinetic version of the magnetorotational instability explored extensively as a mechanism for magnetic field amplification and angular momentum transport in accretion disks. The kinetic calculation is appropriate for hot accretion flows onto compact objects and for the growth of very weak magnetic fields, where the collisional mean free path is larger than the wavelength of the unstable modes. We show that the kinetic instability criterion is the same as in MHD, namely that the angular velocity decrease outward. However, nearly every mode has a linear kinetic growth rate that differs from its MHD counterpart. The kinetic growth rates also depend explicitly on β, i.e., on the ratio of the gas pressure to the pressure of the seed magnetic field. For β~1 the kinetic growth rates are similar to the MHD growth rates, while for β>>1 they differ significantly. For β>>1, the fastest growing mode has a growth rate ~sqrt(3)Ω for a Keplerian disk, larger than its MHD counterpart; there are also many modes whose growth rates are negligible, <~β-1/2Ω<<Ω. We provide a detailed physical interpretation of these results and show that gas pressure forces, rather than just magnetic forces, are central to the behavior of the magnetorotational instability in a collisionless plasma. We also discuss the astrophysical implications of our analysis.

  17. Increasing Running Step Rate Reduces Patellofemoral Joint Forces

    PubMed Central

    Lenhart, Rachel L.; Thelen, Darryl G.; Wille, Christa M.; Chumanov, Elizabeth S.; Heiderscheit, Bryan C.

    2013-01-01

    Purpose Increasing step rate has been shown to elicit changes in joint kinematics and kinetics during running, and has been suggested as a possible rehabilitation strategy for runners with patellofemoral pain. The purpose of this study was to determine how altering step rate affects internal muscle forces and patellofemoral joint loads, and then to determine what kinematic and kinetic factors best predict changes in joint loading. Methods We recorded whole body kinematics of 30 healthy adults running on an instrumented treadmill at three step rate conditions (90%, 100%, and 110% of preferred step rate). We then used a 3D lower extremity musculoskeletal model to estimate muscle, patellar tendon, and patellofemoral joint forces throughout the running gait cycles. Additionally, linear regression analysis allowed us to ascertain the relative influence of limb posture and external loads on patellofemoral joint force. Results Increasing step rate to 110% of preferred reduced peak patellofemoral joint force by 14%. Peak muscle forces were also altered as a result of the increased step rate with hip, knee and ankle extensor forces, and hip abductor forces all reduced in mid-stance. Compared to the 90% step rate condition, there was a concomitant increase in peak rectus femoris and hamstring loads during early and late swing, respectively, at higher step rates. Peak stance phase knee flexion decreased with increasing step rate, and was found to be the most important predictor of the reduction in patellofemoral joint loading. Conclusion Increasing step rate is an effective strategy to reduce patellofemoral joint forces and could be effective in modulating biomechanical factors that can contribute to patellofemoral pain. PMID:23917470

  18. Awakening to Opportunities in International Business: A Title VI-B Grant and Partnership Approach

    ERIC Educational Resources Information Center

    Haynes, Joan; Rutz, Rebecca

    2007-01-01

    Subsequent to hurricane Katrina, Mississippi Gulf Coast businesses are rebuilding and rewriting rules. This catastrophic event has offered the coast a tremendous opportunity to write new rules furthering the redevelopment and expansion of international trade through the ports of Gulfport and Pascagoula and the expansion of the tourism industry.

  19. Sustainable Community Case Study: An Assessment of EPA’s Sustainable Development Plan for Stella, Missouri

    EPA Science Inventory

    In 2006, citizens of Stella, Missouri asked the EPA for technical assistance in demolition and site remediation of an abandoned hospital; and how to redevelop the site to help the community be more sustainable. EPA Region 7 teamed with EPA Office of Research and Development (ORD...

  20. Strip malls, city trees, and community values

    Treesearch

    Kathleen L. Wolf

    2009-01-01

    Strip malls (also known as mini-malls) are a common urban land use, historically promoted by U.S. zoning practices that concentrate retail and commercial development in a narrow band along arterials and major streets. More recently, communities are redeveloping mini-mall zones, expanding landscape plantings as biotechnology, and attempting to create a sense of place....

  1. A Global Outreach Plan for Colleges

    ERIC Educational Resources Information Center

    Skorton, David J.

    2007-01-01

    Speaking at a Harvard University commencement 60 years ago, Secretary of State George C. Marshall proposed a massive program of aid and redevelopment--since known as the Marshall Plan--to bring a war-ravaged Europe back to economic health, political stability, and peace. Today colleges and universities need a new such plan, with university…

  2. Ten Year Plan for the Redevelopment of Intellectual Disability Services. Final Report.

    ERIC Educational Resources Information Center

    Neilson Associates Pty. Ltd., Melbourne (Australia).

    This report recommends a 10-year plan for changes in services to people with intellectual disabilities in Victoria, Australia. Intended key outcomes of the plan include: increases in the numbers of clients receiving direct residential support in community-based accommodations; reductions in numbers of adult clients resident in large scale…

  3. Protected by Design

    ERIC Educational Resources Information Center

    Beck, Darren

    2012-01-01

    Since Pen Argyl High School in Pen Argyl, Pennsylvania was in the process of redeveloping its mascot, the Green Knight, the author decided to implement a studio project based on armor. In groups of three, students would research an assigned type of historical armor, replicate it in cardboard, and fit it to a group member. The lesson had heavy…

  4. 32 CFR 176.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 1 2012-07-01 2012-07-01 false HUD's negotiations and consultations with the LRA. 176.25 Section 176.25 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE... REDEVELOPMENT AND HOMELESS ASSISTANCE § 176.25 HUD's negotiations and consultations with the LRA. HUD may...

  5. 32 CFR 176.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 1 2014-07-01 2014-07-01 false HUD's negotiations and consultations with the LRA. 176.25 Section 176.25 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE... REDEVELOPMENT AND HOMELESS ASSISTANCE § 176.25 HUD's negotiations and consultations with the LRA. HUD may...

  6. 32 CFR 176.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 1 2010-07-01 2010-07-01 false HUD's negotiations and consultations with the LRA. 176.25 Section 176.25 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE... REDEVELOPMENT AND HOMELESS ASSISTANCE § 176.25 HUD's negotiations and consultations with the LRA. HUD may...

  7. 32 CFR 176.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 1 2013-07-01 2013-07-01 false HUD's negotiations and consultations with the LRA. 176.25 Section 176.25 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE... REDEVELOPMENT AND HOMELESS ASSISTANCE § 176.25 HUD's negotiations and consultations with the LRA. HUD may...

  8. 32 CFR 176.25 - HUD's negotiations and consultations with the LRA.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 1 2011-07-01 2011-07-01 false HUD's negotiations and consultations with the LRA. 176.25 Section 176.25 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE... REDEVELOPMENT AND HOMELESS ASSISTANCE § 176.25 HUD's negotiations and consultations with the LRA. HUD may...

  9. Voucher Users and Revitalized Public-Housing Residents 6 Years after Displacement

    ERIC Educational Resources Information Center

    Brooks, Fred; Lewinson, Terri; Aszman, Jennifer; Wolk, Jim

    2012-01-01

    Objective: A total of 6 years after displacement by a Housing Opportunities for People Everywhere (HOPE VI) project, this research examines residents who returned to the redeveloped community and residents who decided to keep their vouchers and were living in private sector housing. Respondents were compared on the following variables: application…

  10. ACRES - Brownfields Properties

    EPA Pesticide Factsheets

    Brownfields are real property, the expansion, redevelopment, or reuse of which may be complicated by the presence or potential presence of a hazardous substance, pollutant or contaminant. This dataset shows the locations of sites, facilities and properties that have been contaminated by hazardous materials and are being, or have been, cleaned up under EPA Brownfields cleanup programs.

  11. A CRITICAL APPRAISAL OF RETRAINING AND RELOCATION OF UNEMPLOYED WORKERS IN DISTRESSED AREAS.

    ERIC Educational Resources Information Center

    SOMERS, GERALD G.

    THIS FORD FOUNDATION SPONSORED RESEARCH PROJECT CONCENTRATED ON EVALUATING AND REPORTING ON PROGRAMS ESTABLISHED UNDER THE AREA REDEVELOPMENT ACT OF 1961 TO RETRAIN AND RELOCATE UNEMPLOYED WORKERS IN FOUR DEPRESSED AREAS OF WEST VIRGINIA. RESEARCHERS, SCHOLARS, AND RESOURCE PERSONS WERE INVOLVED IN CONDUCTING THE WEST VIRGINIA PHASE OF THE PROJECT…

  12. Higher Education and Reentry: The Gifts They Bring. Reentry Research in the First Person

    ERIC Educational Resources Information Center

    Halkovic, Alexis; Fine, Michelle; Bae, John; Campbell, Leslie; Evans, Desheen; Gary, Chaka; Greene, Andrew; Ramirez, Marc; Riggs, Robert; Taylor, Michael; Tebout, Ray; Tejawi, Aenora

    2013-01-01

    Virtually every study of the impact of college on incarcerated and formerly incarcerated people demonstrates a positive effect on income, civic engagement, family, and personal health, as well as dramatically reduced recidivism rates. College in and after prison offers a singularly effective strategy for redeveloping individuals, families and…

  13. 3 CFR 8967 - Proclamation 8967 of April 30, 2013. National Building Safety Month, 2013

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... States of America A Proclamation When natural disasters and other hazards put American lives at risk... redevelopment. To get involved, visit www.Ready.gov. Time and again, devastating natural disasters have tested... stakeholders across our country to adopt disaster-resistant building codes and standards. We are collaborating...

  14. 24 CFR 586.20 - Overview of the process.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... either on or off the installation; (C) Learn about the LRA's process and schedule for receiving notices... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Overview of the process. 586.20...-COMMUNITY REDEVELOPMENT AND HOMELESS ASSISTANCE § 586.20 Overview of the process. (a) Recognition of the LRA...

  15. 76 FR 8720 - Record of Decision for the Disposal and Reuse of Naval Air Station Brunswick, ME

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ... Drozd, BRAC Program Management Office (PMO) Northeast, 4911 Broad Street, Building 679, Philadelphia... Redevelopment Authority (BLRA). Full build-out of the Preferred Alternative is proposed to be implemented over a... installation, including a more intensively built environment, new land uses, and open public access to the...

  16. Redeveloping Nicosia International Airport: An Extroverting Y2 Group Design Project

    ERIC Educational Resources Information Center

    Yiatros, S.

    2017-01-01

    This article follows the timeline of the "Nicosia International Airport: The Return" Integrated Design Project which was undertaken by Year 2 students of Civil Engineering and Geomatics at the Cyprus University of Technology in Cyprus. The Nicosia International Airport was the first and main airport of the Republic of Cyprus since its…

  17. Authentic Student Inquiry: The Mismatch between the Intended Curriculum and the Student-Experienced Curriculum

    ERIC Educational Resources Information Center

    Hume, Anne; Coll, Richard

    2010-01-01

    As a means of achieving scientific literacy goals in society, the last two decades have witnessed international science curriculum redevelopment that increasingly advocates a "new look" inquiry-based approach to learning. This paper reports on the nature of the student-experienced curriculum where secondary school students are learning…

  18. Engineering properties of douglas-fir lumber reclaimed from deconstructed buildings

    Treesearch

    Robert Falk; Derek Maul; Steven Cramer; James Evans; Victoria Herian

    2008-01-01

    A vast wood resource exists in our Nation's wood-framed building infrastructure. As the buildings in this infrastructure age and are remodeled or removed for redevelopment, the wood framing residing in these buildings has the potential to be recovered for reuse. However, little technical information exists on the residual engineering properties of reclaimed...

  19. Brownfield redevelopment: a hidden opportunity for conservation biology

    Treesearch

    Lynne M. Westphal; Jeffery M. Levengood; Alaka Wali; David Soucek; Douglas F. Stotz

    2005-01-01

    Brownfields - lands that are idle due to concerns about contamination - are often prominent features of urban areas. Conservation in an urbanizing world must take brownfields into consideration because regions of heavy industry can harbor areas of ecological significance. The Calumet region of northwest Indiana and northeast Illinois is one such place, where the...

  20. FROM BROWNFIELDS TO GREEN STREETS - A PROPOSAL TO DEVELOP A MULTI-AGENCY COLLABORATIVE DECISION-MAKING PROCESS FOR SOLVING SMALL SCALE BROWNFIELD URBAN REDEVELOPMENT ISSUES

    EPA Science Inventory

    (1) Technical challenge to sustainability: The prevalence of brownfield properties across the United States has reached epidemic proportions. Created from lack of market interest, preference for easily developed land, fear of legal liability, and lack of resources, brownfiel...

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