Science.gov

Sample records for fork flathead watershed

  1. South Fork Flathead Watershed Westslope Cutthroat Trout Conservation Program, Annual Report 2002.

    SciTech Connect

    Grisak, Grant; Marotz, Brian

    2003-06-01

    In 1999, Montana Fish, Wildlife & Parks (MFWP) began a program aimed at conserving the genetically pure populations of westslope cutthroat trout in the South Fork Flathead River drainage. The objective of this program is to eliminate all of the exotic and hybrid trout that threaten the genetically pure westslope cutthroat populations in the South Fork Flathead. The exotic and hybrid trout populations occur in several headwater lakes and their outflow streams. In 2001 MFWP released a draft environmental assessment, pursuant to the Montana Environmental Policy Act (MEPA), that addressed the use of motorized equipment to deliver personnel and materials to some of these lakes in the Bob Marshall and Great Bear Wildernesses (Grisak 2001). After a 30-day public comment period, MFWP determined that the complexity of issues was too great and warranted a more detailed analysis. These issues included transportation options for personnel, equipment and materials, the use of motorized equipment in wilderness, fish removal methods, fish stocking, and the status and distribution of amphibian populations in the project area. Because the program also involves the U.S. Forest Service (USFS) and Bonneville Power Administration (BPA), the environmental analysis needs to comply with the National Environmental Policy Act (NEPA). In October 2001, pursuant to NEPA, MFWP, along with the USFS and BPA initiated an environmental assessment to address these issues. In June 2002, the three agencies determined that the scope of these issues warranted an Environmental Impact Statement. This specialist report describes the logistical, technical and biological issues associated with this project and provides an analysis of options for fish removal, transportation and fish stocking. It further analyzes issues and concerns associated with amphibian populations and creating new domesticated stocks of westslope cutthroat trout. Finally, this document provides a description of each lake, the best

  2. Flathead River Focus Watershed Coordinator, 2002 Annual Report.

    SciTech Connect

    DuCharme, Lynn

    2003-04-01

    The Bonneville Power Administration (BPA) has long been involved with funding of the Cooperative Habitat Protection and Improvement with Private Landowners program in accordance with the Northwest Power Planning Council's (NPPC) Fish & Wildlife Program (Section 7.7). Section 7.7B.1 requires the establishment of ''at least one model watershed coordinator selected by each representative state''. This project was initiated in 1997 with the purpose of fulfilling the NPPC's watershed program within the Flathead River basin in western Montana. Currently, the Flathead watershed has been radically altered by hydropower and other land uses. With the construction of Hungry Horse, Bigfork and Kerr dams, the Flathead River system has been divided into isolated populations. Bull trout have been listed as threatened by the US Fish and Wildlife Service and westslope cutthroat trout have been petitioned for listing. Many streams in the drainage have been destabilized during recent decades. Past legal and illegal species introductions are also causing problems. This project fosters in-kind, out-of-place mitigation to offset the impacts of hydroelectric power to 72 miles of the South Fork of the Flathead River and its tributaries upstream of Hungry Horse Dam. Key subbasins within the Flathead drainage, which are critical to native species restoration, are experiencing rapid changes in land ownership and management direction. Subdivision and residential development of agricultural and timber lands adjacent to waterways in the drainage pose one of the greatest threats to weak but recoverable stocks of trout species. Plum Creek Timber Company, a major landholder in the Flathead drainage is currently divesting itself of large tracks of its lakeshore and streamside holdings. Growth of small tract development throughout the area and its tributaries is occurring at a record rate. Immediate to short-term action is required to protect stream corridors through many of these areas if cost

  3. Flathead River Focus Watershed Coordinator, 2005-2006 Annual Report.

    SciTech Connect

    DuCharme, Lynn

    2006-05-01

    The Bonneville Power Administration (BPA) has long been involved with funding of the Cooperative Habitat Protection and Improvement with Private Landowners program in accordance with the Northwest Power Planning Council's (NPPC) Fish & Wildlife Program (Section 7.7). Section 7.7B.1 requires the establishment of ''at least one model watershed coordinator selected by each representative state''. This project was initiated in 1997 with the purpose of fulfilling the NWPCC's watershed program within the Flathead River basin in western Montana. Currently, the Flathead watershed has been radically altered by hydropower and other land uses. With the construction of Hungry Horse, Bigfork and Kerr dams, the Flathead River system has been divided into isolated populations. Bull trout have been listed as threatened by the US Fish and Wildlife Service and westslope cutthroat trout have been petitioned for listing. Many streams in the drainage have been destabilized during recent decades. Past legal and illegal species introductions are also causing problems. This project fosters in-kind, out-of-place mitigation to offset the impacts of hydroelectric power to 72 miles of the South Fork of the Flathead River and its tributaries upstream of Hungry Horse Dam. Key subbasins within the Flathead drainage, which are critical to native species restoration, are experiencing rapid changes in land ownership and management direction. Subdivision and residential development of agricultural and timber lands adjacent to waterways in the drainage pose one of the greatest threats to weak but recoverable stocks of trout species. Plum Creek Timber Company, a major landholder in the Flathead drainage is currently divesting itself of large tracks of its lakeshore and streamside holdings. Growth of small tract development throughout the area and its tributaries is occurring at a record rate. Immediate to short-term action is required to protect stream corridors through many of these areas if cost

  4. Flathead River Focus Watershed Coordinator, 2004-2005 Annual Report.

    SciTech Connect

    DuCharme, Lynn

    2006-06-26

    The Bonneville Power Administration (BPA) has long been involved with funding of the Cooperative Habitat Protection and Improvement with Private Landowners program in accordance with the Northwest Power Planning Council's (NPPC) Fish & Wildlife Program (Section 7.7). Section 7.7B.1 requires the establishment of ''at least one model watershed coordinator selected by each representative state''. This project was initiated in 1997 with the purpose of fulfilling the NWPCC's watershed program within the Flathead River basin in western Montana. Currently, the Flathead watershed has been radically altered by hydropower and other land uses. With the construction of Hungry Horse, Bigfork and Kerr dams, the Flathead River system has been divided into isolated populations. Bull trout have been listed as threatened by the US Fish and Wildlife Service and westslope cutthroat trout have been petitioned for listing. Many streams in the drainage have been destabilized during recent decades. Past legal and illegal species introductions are also causing problems. This project fosters in-kind, out-of-place mitigation to offset the impacts of hydroelectric power to 72 miles of the South Fork of the Flathead River and its tributaries upstream of Hungry Horse Dam. Key subbasins within the Flathead drainage, which are critical to native species restoration, are experiencing rapid changes in land ownership and management direction. Subdivision and residential development of agricultural and timber lands adjacent to waterways in the drainage pose one of the greatest threats to weak but recoverable stocks of trout species. Plum Creek Timber Company, a major landholder in the Flathead drainage is currently divesting itself of large tracks of its lakeshore and streamside holdings. Growth of small tract development throughout the area and its tributaries is occurring at a record rate. Immediate to short-term action is required to protect stream corridors through many of these areas if cost

  5. Flathead River Focus Watershed Coordinator, 2003-2004 Annual Report.

    SciTech Connect

    DuCharme, Lynn

    2004-06-01

    The Bonneville Power Administration (BPA) has long been involved with funding of the Cooperative Habitat Protection and Improvement with Private Landowners program in accordance with the Northwest Power Planning Council's (NPPC) Fish & Wildlife Program (Section 7.7). Section 7.7B.1 requires the establishment of ''at least one model watershed coordinator selected by each representative state''. This project was initiated in 1997 with the purpose of fulfilling the NWPCC's watershed program within the Flathead River basin in western Montana. Currently, the Flathead watershed has been radically altered by hydropower and other land uses. With the construction of Hungry Horse, Bigfork and Kerr dams, the Flathead River system has been divided into isolated populations. Bull trout have been listed as threatened by the US Fish and Wildlife Service and westslope cutthroat trout have been petitioned for listing. Many streams in the drainage have been destabilized during recent decades. Past legal and illegal species introductions are also causing problems. This project fosters in-kind, out-of-place mitigation to offset the impacts of hydroelectric power to 72 miles of the South Fork of the Flathead River and its tributaries upstream of Hungry Horse Dam. Key subbasins within the Flathead drainage, which are critical to native species restoration, are experiencing rapid changes in land ownership and management direction. Subdivision and residential development of agricultural and timber lands adjacent to waterways in the drainage pose one of the greatest threats to weak but recoverable stocks of trout species. Plum Creek Timber Company, a major landholder in the Flathead drainage is currently divesting itself of large tracks of its lakeshore and streamside holdings. Growth of small tract development throughout the area and its tributaries is occurring at a record rate. Immediate to short-term action is required to protect stream corridors through many of these areas if cost

  6. Hydrologic, water-quality, and biological characteristics of the North Fork Flathead River, Montana, water years 2007-2008

    USGS Publications Warehouse

    Mills, Taylor J.; Schweiger, E. William; Mast, M. Alisa; Clow, David W.

    2012-01-01

    In water year 2007, the U.S. Geological Survey, in cooperation with the National Park Service, began a 2-year study to collect hydrologic, water-quality, and biological data to provide a baseline characterization of the North Fork Flathead River from the United States-Canada border to its confluence with the Middle Fork of the Flathead River near Columbia Falls, Montana. Although mining in the Canadian portion of the North Fork Basin was banned in 2010 by a Memorandum of Understanding issued by the Province of British Columbia, baseline characterization was deemed important for the evaluation of any potential future changes in hydrology, water quality, or aquatic biology in the basin. The North Fork Basin above Columbia Falls (including Canada) drains an area of 1,564 square miles, and the study area encompasses the portion of the basin in Montana, which is 1,126 square miles. Seasonal patterns in the hydrology of the North Fork are dominated by the accumulation and melting of seasonal snowpack in the basin. Low-flow conditions occurred during the late-summer, fall, and winter months, and high-flow conditions coincided with the spring snowmelt. Substantial gains in streamflow occurred along the study reach of the North Fork, 85 percent of which were accounted for by tributary inflows during low-flow conditions, indicating unmeasured streamflow inputs along the main stem were 15 percent or less.

  7. East Fork Watershed Cooperative: Toward better system-scale ...

    EPA Pesticide Factsheets

    The East Fork Watershed Cooperative is a group intent on understanding how to best manage water quality in a large mixed-use Midwestern watershed system. The system contains a reservoir that serves as a source of drinking water and is popular for water recreation. The reservoir is experience harmful algal blooms. The system including the reservoir has become a significant case study for EPA ORD research and development. The Cooperative includes affiliates from the USACE, the OHIO EPA, the USGS, the USDA, and local Soil and Water Conservation districts as well as utility operators and water quality protection offices. The presentation includes a description of the water quality monitoring and modeling program in the watershed, followed by the results of using the watershed model to estimate the costs associated with nutrient reduction to Harsha Lake, and then ends with an explanation of temporal changes observed for important factors controlling harmful algae in Harsha Lake and how this lake relates to other reservoirs in the Ohio River Basin. This presentation is an invited contribution to the Ohio River Basin Water Quality Workshop sponsored by the US ACE and the US EPA. The presentation describes the activities of the East Fork Watershed Cooperative and the knowledge it has gained to help better manage a case study watershed system over the last few years. The East Fork of the Little Miami River is the focal watershed. It is a significant tributary to the Lit

  8. East Fork Watershed Cooperative: Toward better system-scale watershed management

    EPA Science Inventory

    The East Fork Watershed Cooperative is a group intent on understanding how to best manage water quality in a large mixed-use Midwestern watershed system. The system contains a reservoir that serves as a source of drinking water and is popular for water recreation. The reservoir i...

  9. Development of a precipitation-runoff model to simulate unregulated streamflow in the South Fork Flathead River Basin, Montana

    USGS Publications Warehouse

    Chase, K.J.

    2011-01-01

    This report documents the development of a precipitation-runoff model for the South Fork Flathead River Basin, Mont. The Precipitation-Runoff Modeling System model, developed in cooperation with the Bureau of Reclamation, can be used to simulate daily mean unregulated streamflow upstream and downstream from Hungry Horse Reservoir for water-resources planning. Two input files are required to run the model. The time-series data file contains daily precipitation data and daily minimum and maximum air-temperature data from climate stations in and near the South Fork Flathead River Basin. The parameter file contains values of parameters that describe the basin topography, the flow network, the distribution of the precipitation and temperature data, and the hydrologic characteristics of the basin soils and vegetation. A primary-parameter file was created for simulating streamflow during the study period (water years 1967-2005). The model was calibrated for water years 1991-2005 using the primary-parameter file. This calibration was further refined using snow-covered area data for water years 2001-05. The model then was tested for water years 1967-90. Calibration targets included mean monthly and daily mean unregulated streamflow upstream from Hungry Horse Reservoir, mean monthly unregulated streamflow downstream from Hungry Horse Reservoir, basin mean monthly solar radiation and potential evapotranspiration, and daily snapshots of basin snow-covered area. Simulated streamflow generally was in better agreement with observed streamflow at the upstream gage than at the downstream gage. Upstream from the reservoir, simulated mean annual streamflow was within 0.0 percent of observed mean annual streamflow for the calibration period and was about 2 percent higher than observed mean annual streamflow for the test period. Simulated mean April-July streamflow upstream from the reservoir was about 1 percent lower than observed streamflow for the calibration period and about 4

  10. Inferring Causes of Biological Impairment in the Clear Fork Watershed, West Virginia (Final)

    EPA Science Inventory

    The Clear Fork, WV case study illustrates a causal assessment in a watershed using the USEPA stressor identification process described on www.epa.gov/caddis. This case study demonstrates that a watershed-wide causal assessment has several advantages for making analysis practical,...

  11. Inferring Causes of Biological Impairment in the Clear Fork Watershed, West Virginia (Final)

    EPA Science Inventory

    EPA announced the availability of the final report, Inferring Causes of Biological Impairment in the Clear Fork Watershed, West Virginia. This study illustrates a causal assessment in a watershed using the US EPA stressor identification process described on the www.epa....

  12. Fluvial sediment in Salem Fork watershed, West Virginia

    USGS Publications Warehouse

    Flint, R.F.

    1972-01-01

    Suspended sediment discharged from the 8.32-square-mile Salem Fork study area in Harrison County, W. Va., averaged 3,500 tons per year during the first 4-year period of investigation and 1,770 tons per year during the second 4-year period. The difference as attributed to increased flow control, effected by the completion of detention structures and other conservation measures, the absence of appreciable sediment-producing construction activities, and a reduction of the amounts of rainfall and runoff during the second 4-year period. Particle-size distribution of the suspended sediment discharged from the watershed remained unchanged during the two 4-year periods. Although sand and some silt were deposited in upstream reservoirs, sands and other sediments were evidently entrained in the flow below the reservoirs. During the 7.75-year period, reservoir 11A had a trap efficiency of 88 percent. The average annual sediment yield of subwatershed 11A was 1.31 tons per acre, or 837 tons per square mile. Outflow from reservoir 11A occurred during 81 percent of the investigation 'period, October 1954 to June 1962, and 78 percent of the sediment discharge from the reservoir occurred during less than 6 percent of the investigation period. A comparison of particle-size distribution of inflow sediment with that of outflow sediment revealed that practically all sands and some silts entering reservoir 11A were deposited in the reservoir. Chemical analyses of inflow water and the particle-size analyses suggested that flocculation of fine sediments occurred in the reservoir. Analysis of the sediment data collected at the outflow of reservoir 9 during 1956-62 revealed that the average annual sediment discharge was 128,000 pounds per year. Limited particle-size data suggested that practically no sand was discharged from reservoir 9, even though the inflow contained sand. Average annual inflow to reservoirs 11A and 9 compared favorably with average annual runoff for the entire watershed

  13. NUTRIENT CONCENTRATIONS IN FLOWING WATERS OF THE SOUTH FORK BROAD RIVER, GEORGIA WATERSHED

    EPA Science Inventory

    The South Fork Broad River (SFBR) drains about 635 km2 of the Georgia Piedmont. The SFBR watershed is primarily rural and undeveloped although the human population increased by about 25% between 1990 and 2000. Forestry and agriculture are the main land uses. Agriculture consis...

  14. Using Caffeine as a Water Quality Indicator in the Ambient Monitoring Program for Third Fork Creek Watershed, Durham, North Carolina

    PubMed Central

    Spence, Porché L

    2015-01-01

    Caffeine has been suggested as a chemical indicator for domestic wastewater in freshwater systems, although it is not included in water quality monitoring programs. The Third Fork Creek watershed in Durham, NC, is highly urbanized, with a history of receiving untreated wastewater from leaking and overflowing sanitary sewers. The poor water quality originating in the Third Fork Creek watershed threatens its intended uses and jeopardizes drinking water, aquatic life, and recreational activities provided by Jordan Lake. Organic waste contaminants have been detected in both Third Fork Creek watershed and Jordan Lake; however, the sampling periods were temporary, resulting in a few samples collected during nonstorm periods. It is recommended that (1) the concentration of caffeine and other organic waste contaminants are determined during storm and nonstorm periods and (2) caffeine is monitored regularly with traditional water quality indicators to evaluate the health of Third Fork Creek watershed. PMID:26157335

  15. Monitoring, Modeling, and Emergent Toxicology in the East Fork Watershed: Developing a Test Bed for Water Quality Management.

    EPA Science Inventory

    Overarching objectives for the development of the East Fork Watershed Test Bed in Southwestern Ohio include: 1) providing research infrastructure for integrating risk assessment and management research on the scale of a large multi-use watershed (1295 km2); 2) Focusing on process...

  16. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS, AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    EPA Science Inventory

    This technical report provides a description of the field project design, quality control, the sampling protocols and analysis methodology used, and standard operating procedures for the South Fork Broad River Watershed (SFBR) Total Maximum Daily Load (TMDL) project. This watersh...

  17. South Fork Salmon River Watershed Restoration, 2008-2009 Annual Report.

    SciTech Connect

    Reaney, Mark D.

    2009-04-15

    The watershed restoration work elements within the project area, the South Fork Salmon River Watershed, follow the watershed restoration approach adopted by the Nez Perce Tribe Department of Fisheries Resource Management (DFRM) - Watershed Division. The vision of the Nez Perce Tribe DFRM-Watershed Division focuses on protecting, restoring, and enhancing watersheds and treaty resources within the ceded territory of the Nez Perce Tribe under the Treaty of 1855 with the United States Federal Government. The program uses a holistic approach, which encompasses entire watersheds, ridge top to ridge top, emphasizing all cultural aspects and strategies that rely on natural fish production and healthy river ecosystems. The Nez Perce Tribe DFRM-Watershed Division strives towards maximizing historic ecosystem productivity and health for the restoration of anadromous and resident fish populations and the habitat on which all depend on for future generations Originally, this project was funded to create a step/pool stream channel that was appropriate to restore fish passage where the 'Glory Hole Cascade' is currently located at the Stibnite Mine. Due to unforeseen circumstances at the time, the project is unable to move forward as planned and a request for a change in scope of the project and an expansion of the geographic area in which to complete project work was submitted. No additional funds were being requested. The ultimate goal of this project is to work with the holistic, ridge top to ridge top approach to protect and restore the ecological and biological functions of the South Fork Salmon River Watershed to assist in the recovery of threatened and endangered anadromous and resident fish species. FY 2008 Work Elements included two aquatic organism passage (AOP) projects to restore habitat connectivity to two fish-bearing tributaries to the East Fork South Fork Salmon River, Salt and Profile Creeks. The Work Elements also included road survey and assessment activities

  18. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED, GEORGIA

    EPA Science Inventory

    There is an urgent need for EPA to develop protocols for establishing Total Maximum Daily Loads (TMDLs) in streams, lakes and estuaries. A cooperative TMDL field data collection project between ORD and Region 4 is ongoing in the South Fork Broad River Watershed (SFBR), a 245.18 ...

  19. Water resources of the Big Fork River watershed, north-central Minnesota

    USGS Publications Warehouse

    Lindholm, Gerald F.; Helgesen, John O.; Ericson, Donald W.

    1976-01-01

    The Big Fork River watershed is one of 39 watershed units designated by the Minnesota Department of Natural Resources for evaluation of the State 's water resources. Included is an appraisal of the occurrence, quantity, quality, and availability of ground and surface waters. Water resources are not intensively developed anywhere in the watershed. Most development is in the southern half, as much of the northern half is peat-covered wetlands. Essentially all water used is withdrawn from ground-water sources, mainly glacial drift, which ranges from 0 to 250 feet in thickness. Most ground water is of the calcium or sodium bicarbonated type. The degree of mineralization increases down-gradient in the flow system. Groundwater is typically very hard and high in iron and manganese. Lakes and large areas of wetlands have a natural regulating effect on streamflow. Water in streams is of the calcium bicarbonate type. The amount of mineralization at a given time reflects surficial geology, being greatest in streams draining till areas and least in streams draining peatlands. Water drained from peatlands is commonly higher in color and iron and lower in pH than water from areas of mineral soil.

  20. Middle Fork Holston River watershed biological assessment, summers of 1986 and 1987

    SciTech Connect

    Saylor, C.F.; Hill, D.M.; Ahlstedt, S.A.; Brown, A.M.

    1988-05-01

    Fish and macroinvertebrates were collected to obtain a qualitative and quantitative determination relating the impacts of land use activities on aquatic fauna and water quality in watersheds of the Middle Fork Holston River. Samples were compared to historical data, used for calculating an Index of Biological Integrity (IBI), and examined for diversity and abundance of macroinvertebrates. Most IBI's determined for subwatersheds of the Middle Fork Holston River (MFHR) supported land use inventories and soil loss estimates previously completed by TVA in 1985. These inventories targeted streams likely to be impacted by siltation, agricultural runoff, and other nonpoint pollution sources. Findings indicated that siltation and enrichment had increased in the mainstream of MFHR since 1969. Several sites exhibited characteristics implying additional forms of pollution which were impacting the fauna. Benthic macroinvertebrate samples were helpful in documenting degradation but did not always support IBI's and land use inventory and soil loss estimates. Macroinvertebrate fauna are typically more resilient and recover faster than fish fauna, offering a partial explanation for this phenomenon. 6 refs., 3 figs., 12 tabs.

  1. Flathead River Creel Report, 1992-1993. Final Report.

    SciTech Connect

    Hanzel, Delano

    1995-09-01

    A roving creel survey was conducted on the Flathead River system, May 1992 through May 1993, as part of Hungry Horse Dam Fisheries Mitigation, funded by Bonneville Power Administration. The Flathead River system is a tributary to the Clarks Fork of the Columbia River originating in northwest Montana and southern British Columbia. The river creel survey was conducted in conjunction with a Flathead Lake creel survey. This document summarizes the creel survey on the river system. The purpose of these creel surveys was to quantify fishery status prior to mitigation efforts and provide replicative survey methodology to measure success of future mitigation activities. 4 figs., 21 tabs.

  2. Hungry Horse Dam Fisheries Mitigation; Kokanee Stocking and Monitoring in Flathead Lake, 1995 Annual Report.

    SciTech Connect

    Fredenberg, Wade; Carty, Daniel; Cavigli, Jon

    1996-06-01

    The operation of Hungry Horse Dam on the South Fork-of the Flathead River reduced the reproductive success of kokanee (Oncorhynchus nerka) spawning in the Flathead River. Montana Fish, Wildlife and Parks (MFWP) and the Confederated Salish and Kootenai Tribes (CSKT) authored a mitigation plan to offset those losses. The mitigation goal, stated in the Fisheries Mitigation Plan for Losses Attributed to the Construction and Operation of Hungry Horse Dam, is to: {open_quotes}Replace lost annual production of 100,000 kokanee adults, initially through hatchery production and pen rearing in Flathead Lake, partially replacing lost forage for lake trout (Salvelinus namaycush) in Flathead Lake.{close_quotes}

  3. Secure & Restore Critical Fisheries Habitat, Flathead Subbasin, FY2008 Annual Report.

    SciTech Connect

    DuCharme, Lynn; Tohtz, Joel

    2008-11-12

    The construction of Hungry Horse Dam inundated 125 km of adfluvial trout habitat in the South Fork of the Flathead River and its tributaries, impacting natural fish reproduction and rearing. Rapid residential and commercial growth in the Flathead Watershed now threaten the best remaining habitats and restrict our opportunities to offset natural resource losses. Hydropower development and other land disturbances caused severe declines in the range and abundance of our focal resident fish species, bull trout and westslope cutthroat trout. Bull trout were listed as threatened in 1998 under the Endangered Species Act and westslope cutthroat were petitioned for listing under ESA. Westslope cutthroat are a species of special concern in Montana and a species of special consideration by the Confederated Salish and Kootenai Tribes. The Secure & Protect Fisheries Habitat project follows the logical progression towards habitat restoration outlined in the Hungry Horse Dam Fisheries Mitigation Implementation Plan approved by the NWPPC in 1993. This project is also consistent with the 2000 Fish and Wildlife Program and the Flathead River Subbasin Plan that identifies the protection of habitats for these populations as one of the most critical needs in the subbasin and directs actions to offset habitat losses. The Flathead basin is one of the fastest growing human population centers in Montana. Riparian habitats are being rapidly developed and subdivided, causing habitat degradation and altering ecosystem functions. Remaining critical habitats in the Flathead Watershed need to be purchased or protected with conservation easements if westslope cutthroat and bull trout are to persist and expand within the subbasin. In addition, habitats degraded by past land uses need to be restored to maximize the value of remaining habitats and offset losses caused by the construction of Hungry Horse Dam. Securing and restoring remaining riparian habitat will benefit fish by shading and

  4. Lower Flathead System Fisheries Study, Executive Summary, Volume I, 1983-1987 Final Report.

    SciTech Connect

    Cross, David; DosSantos, Joseph M.

    1988-06-01

    This Executive Summary, Volume I, of the lower Flathead System Fisheries Study Final Report, was prepared to provide a study overview for persons who are not fisheries scientists. The contents provide an introduction to the study and its objectives, a short description of the study area, a discussion of the major findings and conclusions of the study, and the description of fisheries management alternatives available to managers of the lower Flathead system. Technical reports were prepared for those portions of the study dealing with the lower Flathead River and its tributaries, Volume II, and the South Bay of Flathead Lake, Volume III. The annual hydrographic regime of the Flathead system, consisting of upper rivers, lake and lower river, has been modified by the construction and operation of two major hydroelectric facilities, Hungry Horse Dam on the south fork Flathead River and Kerr Dam at the outlet of Flathead Lake. The modified hydrographic regime has resulted in significant impacts to kokanee (Oncorhynchus nerka) and several species of trout. Kerr Dam, closed in 1938, controls Flathead Lake levels between 878.7 m (2883 ft) and 881.8 m (2893 ft) and discharges into the lower Flathead River. Kerr Dam is a 63.4 m (208 ft) high concrete arch structure located 7.2 km (4.5 miles) downstream from the outlet of Flathead Lake. The facility is used by Montana Power Company primarily for system frequency load control with some use for low level base load. 77 refs., 5 figs.

  5. A water-quality assessment of the Muddy Fork Silver Creek watershed, Clark, Floyd, and Washington Counties, Indiana

    USGS Publications Warehouse

    Hardy, Mark A.

    1978-01-01

    Data collected for a wide range of flow conditions from September 8, 1975, to July 13, 1976, reveal that human and animal waste loading of streams and pesticides use in the Muddy Fork Silver Creek watershed, Indiana, are probably the most significant water-quality problems. Generally, the type(s) of water in tributary streams in the south and southwest parts of the watershed was calcium bicarbonate and in other tributaries were calcium sulfate and magnesium sulfate. Dissolved-solids concentrations of discharge from top-spill reservoirs were lower and more consistent over a range of flows than concentrations from uncontrolled streams. Concentrations of fecal coliform bacteria and fecal streptococcal bacteria ranged from 5 to 65 ,000 colonies per 100 milliliters and from 5 to 14,000 colonies per 100 milliliters, respectively. Data on periphyton, phytoplankton, and benthic communities collected during low flow in September 1975 indicate organic loading of Muddy Fork downstream from the town of Speed. Phytoplankton community structures varied temporally and spatially. Ranges of concentration (In micrograms per kilogram) of various chlorinated hydrocarbons in samples of bed materials were: chlordane, from 0 to 14; DDT, from 0 to 19; and PCB's, from 0 to 11. Concentrations of aldrin, DDD, DDE, heptachlor, and heptachlor epoxide of 5.1 micrograms per kilogram or less were also detected. The presence of these compounds makes them potentially available for accumulation in the biological food chain. (Woodard-USGS)

  6. A COMPREHENISVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    EPA Science Inventory

    There is an urgent need for EPA to develop protocols for establishing Total Maximum Daily Loads (TMDLs) in streams, lakes and estuaries. A cooperative TMDL field data collection project between ORD and Region 4 is ongoing in the South Fork Broad River Watershed (SFBR), a 245.18 ...

  7. NUTRIENT CONCENTRATIONS IN FLOWING WATERS OF THE SOUTH FORK BROAD RIVER, GEORGIA WATERSHED

    EPA Science Inventory

    We monitored concentrations of nutrients, dissolved organic matter (DOM) and other parameters in 17 headwater streams, at three sites on the main stem, and in three major tributaries near their confluence with the South Fork Broad River on a monthly basis for over a year. Concent...

  8. Quantify Effects of Integrated Land Management on Water Quality in Agricultural Landscape in South Fork Watershed, Iowa River

    NASA Astrophysics Data System (ADS)

    Ha, M.; Wu, M. M.

    2014-12-01

    Sustainable biofuel feedstock production — environmental sustainability and economic sustainability — may be achieved by using a multi-faceted approach. This study focuses on quantifying the water sustainability of an integrated landscaping strategy, by which current land use and land management, cropping system, agricultural Best Management Practices (BMPs), and economics play equal roles. The strategy was applied to the South Fork watershed, IA, including the tributaries of Tipton and Beaver Creeks, which expand to 800-km2 drainage areas. The watershed is an agricultural dominant area covered with row-crops production. On the basis of profitability, switchgrass was chosen as a replacement for row crops in low-productivity land. Areas for harvesting agricultural residue were selected on the basis of soil conservation principals. Double cropping with a cover crop was established to further reduce soil loss. Vegetation buffer strips were in place at fields and in riparian areas for water quality control, resource conservation, and eco service improvement. The Soil and Water Assessment Tool (SWAT) was applied to evaluate source reduction under various management schemes and land use changes. SWAT modeling incorporated 10-yr meteorological information, soil data, land slope classification, land use, four-year crop-rotation cycle, and management operations. Tile drain and pothole parameters were modeled to assess the fate and transport of nutrients. The influence of landscape management and cropping systems on nitrogen and phosphorus loadings, erosion process, and hydrological performance at the sub-watershed scale was analyzed and key factors identified. Results suggest strongly that incorporating agricultural BMPs and conservation strategies into integrated landscape management for certain energy crops in row-crop production regions can be economical and environmentally sustainable.

  9. Comparison of storm response of streams in small, unmined and valley-filled watersheds, 1999-2001, Ballard fork, West Virginia

    USGS Publications Warehouse

    Messinger, Terence

    2003-01-01

    During storms when rainfall intensity exceeded about 1 inch per hour, peak unit runoff from the Unnamed Tributary (surface-mined and filled) Watershed exceeded peak unit runoff from the Spring Branch (unmined) Watershed in the Ballard Fork Watershed in southern West Virginia. During most storms, those with intensity less than about 1 inch per hour, peak unit (area-normalized) flows were greater from the Spring Branch Watershed than the Unnamed Tributary Watershed. One storm that produced less than an inch of rain before flow from the previous storm had receded caused peak unit flow from the Unnamed Tributary Watershed to exceed peak unit flow from the Spring Branch Watershed. Peak unit flow was usually similar in Spring Branch and Ballard Fork. Peak unit flows are expected to decrease with increasing watershed size in homogeneous watersheds; drainage area and proportion of the three watersheds covered by valley fills are 0.19 square mile (mi?) and 44 percent for the Unnamed Tributary Watershed, 0.53 mi? and 0 percent for the Spring Branch Watershed, and 2.12 mi? and 12 percent for the Ballard Fork Watershed. Following all storms with sufficient rainfall intensity, about 0.25 inches per hour, the storm hydrograph from the Unnamed Tributary Watershed showed a double peak, as a sharp initial rise was followed by a decrease in flow and then a delayed secondary peak of water that had apparently flowed through the valley fill. Hortonian (excess overland) flow may be important in the Unnamed Tributary Watershed during intense storms, and may cause the initial peak on the rising arm of storm hydrographs; the water composing the initial peaks may be conveyed by drainage structures on the mine. Ballard Fork and Spring Branch had hydrographs with single peaks, typical of elsewhere in West Virginia. During all storms with 1-hour rainfall greater than 0.75 inches or 24-hour rainfall greater than 1.75 inches during which all stream gages recorded a complete record, the Unnamed

  10. MEETING IN PHILADELPHIA: NUTRIENT CONCENTRATIONS IN FLOWING WATERS OF THE SOUTH FORK BROAD RIVER, GEORGIA WATERSHED

    EPA Science Inventory

    The objective of this poster is by comparing nutrient and DOM concentrations in small and large streams, we hope to better understand: (1) watershed controls on stream nutrient and DOM concentrations; and (2) the variability of nutrient and DOM concentrations within a river netwo...

  11. Sources of acid and metals from the weathering of the Dinero waste pile, Lake Fork watershed, Leadville, Colorado

    USGS Publications Warehouse

    Diehl, S.F.; Hageman, Phil L.; Smith, Kathleen S.; Herron, J.T.; Desborough, G.A.

    2005-01-01

    Two trenches were dug into the south Dinero mine-waste pile near Leadville, Colorado, to study the weathering of rock fragments and the mineralogic sources of metal contaminants in the surrounding wetland and Lake Fork Watershed. Water seeping from the base of the south Dinero waste-rock pile was pH 2.9, whereas leachate from a composite sample of the rock waste was pH 3.3. The waste pile was mostly devoid of vegetation, open to infiltration of precipitation, and saturated at the base because of placement in the wetland. The south mine-waste pile is composed of poorly sorted material, ranging from boulder-size to fine-grained rock fragments. The trenches showed both matrix-supported and clast-supported zones, with faint horizontal color banding, suggesting zonation of Fe oxides. Secondary minerals such as jarosite and gypsum occurred throughout the depth of the trenches. Infiltration of water and transport of dissolved material through the pile is evidenced by optically continuous secondary mineral deposits that fill or line voids. Iron-sulfate material exhibits microlaminations with shrinkage cracking and preferential dissolution of microlayers that evidence drying and wetting events. In addition to fluids, submicron-sized to very fine-grained particles such as jarosite are transported through channel ways in the pile. Rock fragments are coated with a mixture of clay, jarosite, and manganese oxides. Dissolution of minerals is a primary source of metals. Skeletal remnants of grains, outlined by Fe-oxide minerals, are common. Potassium jarosite is the most abundant jarosite phase, but Pb-and Ag-bearing jarosite are common. Grain-sized clusters of jarosite suggest that entire sulfide grains were replaced by very fine-grained jarosite crystals. The waste piles were removed from the wetland and reclaimed upslope in 2003. This was an opportunity to test methods to identify sources of acid and metals and metal transport processes within a waste pile. A series of

  12. 78 FR 23741 - Flathead Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Flathead Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The Flathead Resource Advisory Committee will meet in Kalispell, Montana. The...

  13. 77 FR 48126 - Flathead Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Flathead Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The Flathead Resource Advisory Committee will meet in Kalispell, Montana. The...

  14. Data summary and loading sources for selected water-quality characteristics of streams in blowdown areas, North Fork Elk River Watershed, Colorado, March 1999-August 2000

    USGS Publications Warehouse

    Leib, Kenneth J.; Von Guerard, Paul

    2003-01-01

    On October 25, 1997, in and around the Routt National Forest and the Mount Zirkel Wilderness Area, a winter snowstorm occurred in conjunction with heavy winds that resulted in the destruction of thousands of acres of old-growth tree stands. The U.S. Geological Survey, in cooperation with the U.S. Forest Service, collected water-quality data at five sites in the North Fork Elk River watershed where most of the blowdown occurred. In this report, these data are used to characterize water quality in areas affected by the blowdown compared to that of relatively undisturbed areas. No difference was detected between loads of the selected constituents from the five water-quality sampling sites.

  15. Use of a watershed-modeling approach to assess hydrologic effects of urbanization, North Fork Pheasant Branch basin near Middleton, Wisconsin

    USGS Publications Warehouse

    Steuer, Jeffrey J.; Hunt, R.J.

    2001-01-01

    The North Fork Pheasant Branch Basin in Dane County, Wisconsin is expected to undergo development. There are concerns that development will adversely affect water resources with increased flood peaks, increased runoff volumes, and increased pollutant loads. To provide a scientific basis for evaluating the hydrologic system response to development the Precipitation Runoff Modeling System (PRMS) was used to model the upper Pheasant Branch Creek watershed with an emphasis on the North Fork Basin. The upper Pheasant Branch Creek (18.3 mi2; 11,700 acres) Basin was represented with 21 Hydrologic Response Units (daily time step) and 50 flow planes (5-minute time steps). Precipitation data from the basin outlet streamflow-gaging station located at Highway 12 and temperature data from a nearby airport were used to drive the model. Continuous discharge records at three gaging stations were used for model calibration. To qualitatively assess model representation of small subbasins, periodic reconnaissance, often including a depth measurement, was made after precipitation to determine the occurrence of flow in ditches and channels from small subbasins. As a further effort to verify the model on a small subbasin scale, continuous-stage sensors (15-minute intervals) measured depth at the outlets of three small subbasins (500 to 1,200 acres). Average annual precipitation for the simulation period from 1993 to 1998 was 35.2 inches. The model simulations showed that, on average, 23.9 inches were intercepted by vegetation, or lost to evapotranspiration, 6.0 inches were infiltrated and moved to the regional ground-water system, and 4.8 inches contributed to the upper Pheasant Branch streamflow. The largest runoff event during the calibration interval was in July 1993 (746 ft3/sec; with a recurrence interval of approximately 25 years). Resulting recharge rates from the calibrated model were subsequently used as input into a ground-water-flow model. Average annual recharge varied

  16. Lower Flathead System Fisheries Study, 1984 Annual Report.

    SciTech Connect

    Darling, James E.; Pajak, Paul; Wunderlich, Mary P.

    1984-12-01

    This study was undertaken to assess the effects of Kerr Dam operations on the fisheries of the Lower Flathead System. Supported by Bonneville Power Administration funding, and conducted by the Confederated Salish and Kootenai Tribes, the study began in December of 1982 and is scheduled for completion in December of 1987. This report covers the 1983-84 field season and includes the status of target fish species populations in the Flathead River and tributaries, and initial work in South Bay of Flathead Lake. Additionally it addresses how Kerr operations may effect the reproduction of salmonids and northern pike. Combined trout population estimates for rainbow, brown, brook, and bull trout, averaged 13 fish/km of the lower Flathead River. The number of bull trout and cutthroat trout captured was so low that estimation of their individual populations was not possible. An interim closure to trout harvest on the lower Flathead River was recommended and approved by the Tribal Council until study results can be further analyzed and management options reviewed. Population estimates for northern pike ranged from six/kilometer in poorer habitat, to one hundred three/km in the best habitat in the main Flathead River. Seven pike were radio tagged and their movements monitored. Movements of over 89 km were recorded. One fish left the Flathead River and moved down the Clark Fork to the Plains area. Fish weirs were constructed on the Jocko River and Mission Creek to assess spawning runs of trout from the main river. Thirty-two adult rainbow passed the Jocko weir and twenty-eight passed the Mission weir during the spring spawning season. Twenty adult brown trout were captured at the Jocko weir and five at Mission weir in the fall. The Jocko weir suffered minor damage due to bed load movement during high flows of spring runoff. The structure of trout populations in the lower Flathead River points to spawning and recruitment problems caused by hydroelectric operations and

  17. Model Watershed Plan; Lemhi, Pahsimeroi, and East Fork of the Salmon River Management Plan, 1995 Technical Report.

    SciTech Connect

    Swift, Ralph

    1995-11-01

    Idaho`s Model Watershed Project was established as part of the Northwest Power Planning Council`s plan for salmon recovery in the Columbia River Basin. The Council`s charge was simply stated and came without strings. The tasks were to identify actions within the watershed that are planned or needed for salmon habitat, and establish a procedure for implementing habitat-improvement measures. The Council gave the responsibility of developing this project to the Idaho Soil Conservation Commission. This Model Watershed Plan is intended to be a dynamic plan that helps address these two tasks. It is not intended to be the final say on either. It is also not meant to establish laws, policies, or regulations for the agencies, groups, or individuals who participated in the plan development.

  18. Linking habitat quality with trophic performance of steelhead along forest gradients in the South Fork Trinity River Watershed, California

    USGS Publications Warehouse

    McCarthy, Sarah G.; Duda, Jeffrey J.; Emlen, John M.; Hodgson, Garth R.; Beauchamp, David A.

    2009-01-01

    We examined invertebrate prey, fish diet, and energy assimilation in relation to habitat variation for steelhead Oncorhynchus mykiss (anadromous rainbow trout) and rainbow trout in nine low-order tributaries of the South Fork Trinity River, northern California. These streams spanned a range of environmental conditions, which allowed us to use bioenergetics modeling to determine the relative effects of forest cover, stream temperature, season, and fish age on food consumption and growth efficiency. Evidence of seasonal shifts in reliance on aquatic versus terrestrial food sources was detected among forest cover categories and fish ages, although these categories were not robust indicators of O. mykiss condition and growth efficiency. Consumption estimates were generally less than 20% of maximum consumption, and fish lost weight in some streams during summer low-flow conditions when stream temperatures exceeded 15°C. Current 100-year climate change projections for California threaten to exacerbate negative growth patterns and may undermine the productivity of this steelhead population, which is currently not listed as endangered or threatened. To demonstrate the potential effect of global warming on fish growth, we ran three climate change scenarios in two representative streams. Simulated temperature increases ranging from 1.4°C to 5.5°C during the summer and from 1.5°C to 2.9C during the winter amplified the weight loss; estimated average growth for age-1 fish was 0.4–4.5 times lower than normal (low to high estimated temperature increase) in the warm stream and 0.05–0.8 times lower in the cool stream. We conclude that feeding rate and temperature during the summer currently limit the growth and productivity of steelhead and rainbow trout in low-order streams in the South Fork Trinity River basin and predict that climate change will have detrimental effects on steelhead growth as well as on macroinvertebrate communities and stream ecosystems in general.

  19. Surface seiches in Flathead Lake

    NASA Astrophysics Data System (ADS)

    Kirillin, G.; Lorang, M. S.; Lippmann, T. C.; Gotschalk, C. C.; Schimmelpfennig, S.

    2014-12-01

    Standing surface waves or seiches are inherent hydrodynamic features of enclosed water bodies. Their two-dimensional structure is important for estimating flood risk, coastal erosion and bottom sediment transport and for understanding shoreline habitats and lake ecology in general. In this work, we present analysis of two-dimensional seiche characteristics in Flathead Lake, Montana, USA, a large intermountain lake known to have high seiche amplitudes. To examine spatial characteristics of different seiche modes we used the original procedure of determining the seiche frequencies from the primitive equation model output with subsequent derivation of the spatial seiche structure at fixed frequencies akin the tidal harmonic analysis. The proposed procedure revealed specific seiche oscillation features in Flathead Lake including maximum surface level amplitudes of the first fundamental mode in straights around the largest island; several higher modes appearing locally in the vicinity of the river inflow; the "Helmholtz" open harbor mode, with the period approximately twice that of the longest seiche mode, generated by a large shallow bay connected to the main lake basin; and several rotating seiche modes potentially affecting the lake-wide circulation. We discuss the lake management problems related to of the spatial seiche distribution, such as shoreline erosion, floods and transport of sediments and invasive species in Flathead Lake.

  20. Surface seiches in Flathead Lake

    NASA Astrophysics Data System (ADS)

    Kirillin, G.; Lorang, M. S.; Lippmann, T. C.; Gotschalk, C. C.; Schimmelpfennig, S.

    2015-06-01

    Standing surface waves or seiches are inherent hydrodynamic features of enclosed water bodies. Their two-dimensional structure is important for estimating flood risk, coastal erosion, and bottom sediment transport, and for understanding shoreline habitats and lake ecology in general. In this work, we present analysis of two-dimensional seiche characteristics in Flathead Lake, Montana, USA, a large intermountain lake known to have high seiche amplitudes. To examine spatial characteristics of different seiche modes, we used the original procedure of determining the seiche frequencies from the primitive equation model output with subsequent derivation of the spatial seiche structure at fixed frequencies akin to the tidal harmonic analysis. The proposed procedure revealed specific seiche oscillation features in Flathead Lake, including maximum surface level amplitudes of the first fundamental mode in straights around the largest island; several higher modes appearing locally in the vicinity of the river inflow; the "Helmholtz" open harbor mode, with the period approximately twice that of the longest seiche mode, generated by a large shallow bay connected to the main lake basin; and several rotating seiche modes potentially affecting the lake-wide circulation. We discuss lake management problems related to the spatial seiche distribution, such as shoreline erosion, floods, and transport of sediments and invasive species in Flathead Lake.

  1. 78 FR 45496 - Flathead Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    ... Forest Service Flathead Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The Flathead Resource Advisory Committee will meet in Kalispell, Montana on the dates..., 2013--4:00 p.m. to 6:30 p.m. All Resource Advisory Committee meetings are subject to change...

  2. Lower Flathead System Fisheries Study, 1986 Interim Report.

    SciTech Connect

    Bradshaw, William H.; DosSantos, Joseph M.; Darling, James M.

    1986-08-01

    We believe our results have clearly shown Kerr hydroelectric operations and operational constraints have negatively affected Flathead River trout and northern pike populations and the aquatic habitat which support them. Even so, it is possible to mitigate many of these impacts and develop a very important fishery. Trout abundance in the lower Flathead averaged only 19 fish per kilometer, the lowest abundance of trout for a river of this size in Montana. Little main channel spawning by trout was observed and most spawning probably occurs in tributaries. Lower river tributaries support resident populations of brook, rainbow, brown, and cutthroat trout; and a small resident population of bull trout is present in the South Fork of the Jocko River. Using weirs, spawning runs of rainbow and brown trout from the main river were monitored entering the Jocko River and the Post/Mission Creek system. Utilization of Crow Creek by main river trout stocks of trout was limited to the 6 km segment below Crow Dam. Evaluations of tributary spawning gravels showed high levels of silt which would suggest poor survival of trout eggs. Excessive harvest in the tributaries was indicated by analysis of age class structure and abundance of trout greater than 200 mm.

  3. Supplement Analysis for the Watershed Management Program EIS (DOE/EIS-0265/SA-109) - East Fork Holistic Restoration – Salmon River East Fork (SEF) 12 and Herd Creek (HC) 1

    SciTech Connect

    Stewart, Shannon C.

    2003-07-22

    The Bonneville Power Administration (BPA) is proposing to fund activities that will improve fish passage at Salmon River East Fork diversion 12 and at Herd Creek diversion 1. These projects represent cooperative efforts between the Custer Soil and Water Conservation District, Idaho Department of Fish and Game, and local irrigators. The goal of the SEF 12 project is to improve fish passage and habitat by removing the existing SEF 12 pushup gravel diversion, replacing it with a permanent rock weir structure, and installing an impervious membrane and geotextile liner in the wing diversion parallel to the stream bank. The work on the diversion structure at HC 1 will be confined to the existing headgate, wasteway and plunge pool immediately below the structure. The wasteway and plunge pool are part of the system that provides fish passage around the existing diversion structure. The new structure will include improved stop logs and a reconstructed plunge pool, which will enhance fish passage at the diversion.

  4. Flathead River Basin Hydrologic Observatory, Northern Rocky Mountains

    NASA Astrophysics Data System (ADS)

    Woessner, W. W.; Running, S. W.; Potts, D. F.; Kimball, J. S.; Deluca, T. H.; Fagre, D. B.; Makepeace, S.; Hendrix, M. S.; Lorang, M. S.; Ellis, B. K.; Lafave, J.; Harper, J.

    2004-12-01

    We are proposing the 22, 515 km2 glacially-sculpted Flathead River Basin located in Montana and British Columbia as a Hydrologic Observatory. This hydrologic landscape is diverse and includes large pristine watersheds, rapidly developing intermountain valleys, and a 95 km2 regulated reservoir and 510 km2 lake. The basin has a topographic gradient of over 2,339 m, and spans high alpine to arid climatic zones and a range of biomes. Stream flows are snow-melt dominated and underpinned by groundwater baseflow. The site headwaters contain 37 glaciers and thousands of square kilometers of watersheds in which fire and disease are the only disturbances. In contrast, the HO also contains watersheds at multiple scales that were dominated by glaciers within the last 100 years but are now glacier free, impacted by timber harvests and fires of varying ages to varying degrees, modified by water management practices including irrigation diversion and dams, and altered by development for homes, cities and agriculture. This Observatory provides a sensitive monitor of historic and future climatic shifts, air shed influences and impacts, and the consequences of land and water management practices on the hydrologic system. The HO watersheds are some of the only pristine watersheds left in the contiguous U.S.. They provide critical habitat for key species including the native threaten bull trout and lynx, and the listed western cutthroat trout, bald eagle, gray wolf and the grizzly bear. For the last several thousand years this system has been dominated by snow-melt runoff and moderated by large quantities of water stored in glacial ice. However, the timing and magnitude of droughts and summer flows have changed dramatically. With the information that can be gleaned from sediment cores and landscape records at different scales, this HO provides scientists with opportunities to establish baseline watershed conditions and data on natural hydrologic variability within the system. Such a

  5. Along Middle Fork Road toward North Fork of the Crazy ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Along Middle Fork Road toward North Fork of the Crazy Woman Creek Bridge, view to west - North Fork of Crazy Woman Creek Bridge, Spanning North Fork of Crazy Woman Creek at Middle Fork Road, Buffalo, Johnson County, WY

  6. Seismic reflection study of Flathead Lake, Montana

    USGS Publications Warehouse

    Wold, Richard J.

    1982-01-01

    A seismic reflection survey of Flathead Lake, Montana, was carried out in 1970 to study the geologic structure underlying the lake. Approximately 200 km of track lines were surveyed resulting in about 140 km of useable data (Fig. 1). A one cu. in. air gun was used as the energy source. Navigation was by a series of theodolite sitings of the boat from pairs of shore-based control points. 

  7. Effects of Water Levels on Productivity of Canada Geese in the Northern Flathead Valley, 1984 Annual Report.

    SciTech Connect

    Casey, Daniel

    1985-02-01

    Operation of Hungry Horse Dam on the South Fork Flathead River causes sporadic level fluctuations along the main stem Flathead River. Seasonal water level fluctuations and substantial habitat losses have occurred as a result of construction and operation of Kerr Dam, which regulates Flathead Lake. These fluctuations may impact goose populations through flooding or erosion of nesting and brood-rearing habitats, and increased susceptibility of nests and young to predation. The number, location, and success of goose nests were determined through pair surveys and nest searches. Counts of indicated pairs suggest there were 73-125 occupied nests in the study area; 44 were located in 1984. Twenty were island ground nests, 19 were tree nests, and 5 were on man-made structures. Hatching success was 76 percent. Sixty-one percent of all nests were in deciduous forest habitat; 87 percent were on riparian bench or island landforms. Seventy-four percent of all nests were within 5 m of the seasonal high water mark (HWM) and 85 percent of ground nests were 1 m or less above the HWM. Production, habitat use, and distribution of broods were documented through aerial, boat, ground, and observation tower surveys. 28 refs., 10 figs., 4 tabs.

  8. Effects of the Operation of Kerr and Hungry Horse Dams on the Kokanee Fishery in the Flathead River System, 1979-1985 Final Research Report.

    SciTech Connect

    Clancy, Patrick

    1986-05-01

    This study was undertaken to assess the effects of the operation of Hungry Horse Dam on the kokanee fishery in the Flathead River system. Studies concerning operation of the dam on the Flathead River aquatic biota began in 1979 and continued to 1982 under Bureau of Reclamation funding. These studies resulted in flow recommendations for the aquatic biota in the main stem Flathead River, below the influence of Hungry Horse Dam on the South Fork. Studies concerned specifically with kokanee salmon have continued under Bonneville Power Administration funding since 1982. This completion report covers the entire study period (September 1979 to June 1985). Major results of this study were: (1) development and refinement of methods to assess hydropower impacts on spawning and incubation success of kokanee; (2) development of a model to predict kokanee year class strength from Flathead River flows; and (3) implementation of flows favorable for successful kokanee reproduction. A monitoring program has been developed which will assess the recovery of the kokanee population as it proceeds, and to recommend management strategies to maintain management goals for the kokanee fishery in the river system.

  9. Coastal coho salmon research in the West Fork Smith River: Patterns of coho salmon size and survival within a complex watershed

    EPA Science Inventory

    Effective habitat restoration planning requires the ability to anticipate fish population responses to altered habitats. The EPA has conducted network-scale research to document habitat-specific growth and survival of juvenile salmonids in a complex watershed. These findings ha...

  10. Effects of Water Levels on Productivity of Canada Geese in the Northern Flathead Valley, 1985 Annual Report.

    SciTech Connect

    Casey, Daniel

    1986-04-01

    Operation of Hungry Horse Dam on the South Fork Flathead River causes sporadic water level fluctuations along the main stem Flathead River. Changes in chronology of seasonal water level fluctuations and substantial habitat losses have occurred as a result of construction and operation of Kerr Dam, which regulates Flathead Lake. These fluctuations may impact goose populations through flooding and erosion of nesting and brood-rearing habitats, and increased susceptibility of nests and young to predation. The number, location, and success of goose nests were determined through pair surveys and nest searches. Our 1985 pair count data indicated that 95 to 143 nests may have been present. Hatching success for 1985 nests (55%) was low compared to long-term averages for the region. Predation was the predominant cause of ground nest failure (25 nests); we documented 2 nest failures due to flooding. The maximum gosling count in the study area for 1985 was 197. Six key brood-rearing areas were identified. Most (80%) sites were located in the herbaceous or pasture cover type and the riparian bench landform. Analysis of aerial photographs taken prior to construction of Kerr Dam documented the loss of 1859 acres of habitat along the north shore of Flathead Lake. Losses were attributed to inundation and to continuing erosion due to operation of Kerr Dam. Lake and river water level regimes were compared with the chronology of important periods in the nesting cycle. Low lake levels in May and early June coincide with the breed-rearing period. Mudflats are heavily used by broods, but their effect on survival must still be documented. Preliminary recommendations to protect and enhance Canada goose habitat and production are being developed.

  11. Simulation of streamflow and the effects of brush management on water yields in the Double Mountain Fork Brazos River watershed, western Texas 1994–2013

    USGS Publications Warehouse

    Harwell, Glenn R.; Stengel, Victoria G.; Bumgarner, Johnathan R.

    2016-04-20

    The calibrated watershed model was used to perform brush-management simulations. The National Land Cover Database 2006, which was the land-cover data used to develop the watershed model, was modified to simulate shrubland replacement with grassland in each of the 35 model subbasins. After replacement of shrubland with grassland in areas with land slope less than 20 percent and excluding riparian areas, the modeled 20-year (1994 through 2013) water yields to Lake Alan Henry increased by 114,000 acre-feet or about 5,700 acre-feet per year. In terms of the increase in water yield per acre of shrubland replaced with grassland, the average annual increase in water yield was 17,300 gallons per acre. Within the modeled subbasins, the increase in average annual water yield ranged from 5,850 to 34,400 gallons per acre of shrubland replaced with grassland. Subbasins downstream from the Justiceburg gage had a higher average annual increase in water yield (21,700 gallons per acre) than subbasins upstream from the streamflow-gaging station (16,800 gallons per acre).

  12. Trophic relations of introduced flathead catfish in an atlantic river

    USGS Publications Warehouse

    Baumann, Jessica R.; Kwak, Thomas J.

    2011-01-01

    The flathead catfish Pylodictis olivaris is a large piscivore that is native to the Mississippi and Rio Grande river drainages but that has been widely introduced across the United States. River ecologists and fisheries managers are concerned about introduced flathead catfish populations because of the negative impacts on native fish communities or imperiled species associated with direct predation and indirect competition from this apex predator. We studied the trophic relations of introduced flathead catfish in an Atlantic river to further understand the effects on native fish communities. Crayfish (Astacidea) occurred most frequently in the flathead catfish diet, while sunfish Lepomis spp. comprised the greatest percentage by weight. Neither of two sympatric imperiled fish species (the federally endangered Cape Fear shiner Notropis mekistocholas and the Carolina redhorse Moxostoma sp., a federal species of concern) was found in any diet sample. An ontogenetic shift in diet was evident when flathead catfish reached about 300 mm, and length significantly explained the variation in the percent composition by weight of sunfish and darters Etheostoma and Percina spp. Flathead catfish showed positive prey selectivity for taxa that occupied similar benthic microhabitat, highlighting the importance of opportunistic feeding and prey encounter rates. Flathead catfish displayed a highly variable diel feeding chronology during July, when they had a mean stomach fullness of 0.32%, but then showed a single midday feeding peak during August (mean fullness = 0.52%). The gastric evacuation rate increased between July (0.40/h) and August (0.59/h), as did daily ration, which more than doubled between the 2 months (3.06% versus 7.37%). Our findings increase the understanding of introduced flathead catfish trophic relations and the degree of vulnerability among prey taxa, which resource managers may consider in fisheries management and conservation of native fish populations and

  13. Plant water status relationships among major floodplain sites of the Flathead River, Montana

    USGS Publications Warehouse

    Lee, L.C.; Hinckley, T.M.; Scott, M.L.

    1985-01-01

    Water status measurements of dominant species from major floodplain plant community types of the North Fork Flathead River, Montana were used to test the accuracy of site moisture gradient relationships postulated from floristic ordinations and site water balance estimates. Analysis of variance tests showed significant differences among the average predawn xylem pressure potential (ψp) of species in several community types. However, additional analyses failed to indicate a significant degree of association between averaged predawn Yp measurements and either floristic ordination or site water balance results. Sixty eight percent of 22 trials comparing the diurnal average ψp of the same species in different community types on the same day were less negative for a species in the wetter community types as predicted by floristic ordinations. Similarly, 64% of the trials indicated that the diurnal average stomatal conductance was higher for a species in the wetter type. These results suggest that although a floodplain moisture gradient exists, it alone does not limit the distribution of floodplain plant communities in the North Fork.

  14. The role of episodic fire-related debris flows on long-term (103-104) sediment yields in the Middle Fork Salmon River Watershed, in central Idaho

    NASA Astrophysics Data System (ADS)

    Riley, K. E.; Pierce, J. L.; Hopkins, A.

    2010-12-01

    Episodic fire-related debris flows contribute large amounts of sediment and large woody debris to streams. This study evaluates fire-related sedimentation from small steep tributaries of the Middle Fork Salmon River (MFSR) in central Idaho to evaluate the timing, frequency, and magnitude of episodic fire-related sedimentation on long-term (10 3-10 4) sediment yields. The MFSR lies within the Northern Rocky Mountains and encompasses a range of ecosystems including high elevation (~3,000 -1,700 m) subalpine pine and spruce forests, mid-elevation (2650 - 1130 m) montane Douglas-fir and ponderosa pine-dominated forests and low elevation (~ 1,800 - 900 m) sagebrush steppe. Recent debris flow events in tributaries of the MFSR appear to primarily result from increased surface runoff, rilling, and progressive sediment bulking following high severity fires. This study estimates: 1) the volume of sediment delivered by four recent (1997-2008) fire-related debris flow events using real time kinematic GPS surveys, and 2) the timing of Holocene fire-related debris flow events determined by 14C dating charcoal fragments preserved in buried burned soils and within fire-related deposits. Our measured volumes of the four recent debris flow events are compared to two empirically derived volume estimates based on remotely sensed spatial data (burn severity and slope), measured geometric data (longitudinal profile, cross sectional area, flow banking angle), and precipitation records. Preliminary stratigraphic profiles in incised alluvial fans suggest that a large percentage of alluvial fan thickness is composed of fire-related deposits suggesting fire-related hillslope erosion is a major process delivering sediment to alluvial fans and to the MFSR. Fire-related deposits from upper basins compose ~71% of total alluvial fan thickness, while fire-related deposits from lower basins make up 36% of alluvial fan thickness. However, lower basins are less densely vegetated with small diameter

  15. Flathead River Instream Flow Investigation Project : Final Report 1996-2003.

    SciTech Connect

    Miller, William J.; Ptacek, Jonathan A.

    2003-09-01

    A modified Instream Flow Incremental Methodology (IFIM) approach was used on the mainstem Flathead River from the South Fork Flathead River downstream to Flathead Lake. The objective of this study was to quantify changes in habitat for the target fish species, bull trout (Salvelinus confluentus) and west slope cutthroat trout (Oncorhynchus clarki lewisi), as a function of discharge in the river. This approach used a combination of georeferenced field data for each study site combined with a two-dimensional hydraulic simulation of river hydraulic characteristics. The hydraulic simulations were combined with habitat suitability criteria in a GIS analysis format to determine habitat area as a function of discharge. Results of the analysis showed that habitat area is more available at lower discharges than higher discharges and that in comparison of the pre-dam hydrology with post-dam hydrology, the stable pre-dam baseflows provided more stable habitat than the highly variable flow regime during both summer and winter baseflow post-dam periods. The variability week to week and day to day under post-dam conditions waters and dewaters stream margins. This forces sub-adult fish, in particular bull trout, to use less productive habitat during the night. There is a distinct difference between daytime and nighttime habitat use for bull trout sub-adults. The marginal areas that are constantly wet and then dried provide little in productivity for lower trophic levels and consequently become unproductive for higher trophic levels, especially bull trout sub-adults that use those areas as flows increase. A stable flow regime would be more productive than flow regimes with high variability week to week. The highly variable flows likely put stress on a bull trout subadult and west slope cutthroat trout, due to the additional movement required to find suitable habitat. The GIS approach presented here provides both a visual characterization of habitat as well as Arcview project data

  16. Surface seiches in Flathead Lake, Montana

    NASA Astrophysics Data System (ADS)

    Kirillin, Georgiy; Lorang, Mark; Gotschalk, Chris; Lippmann, Tom

    2013-04-01

    Flathead Lake— the largest freshwater lake in the western USA—undergoes significant short-term water level oscillations due to standing barotropic waves (seiches). Large surface area and several embayments cause high amplitudes and complicated spatial pattern of the seiches. We used water level records from 12 sites distributed around the lake and the results of numerical circulation modeling to establish the modal composition of seiches, the two-dimensional wave shape, and current patterns. For this, we directly applied harmonic analysis to the output of a full circulation model. Compared to the traditional reduced eigenvalue problem, the proposed approach allows modal separation of real datasets that is potentially advantageous for analysis of the resonant response to periodic wind forcing. Surprisingly, both model results and observations demonstrated predominance of the 'two-node' horizontal mode, whereas the usually most acute 'one-node' mode was attenuated by the large shallow bay connected through a narrow straight to the main lake basin. Energy of several higher modes was concentrated around the mouth of the main inflow suggesting their strong effect on the redistribution of the inflow waters and suspended matter within the lake. The rotary spectral analysis revealed rotational character of two particular modes and localized potential upwelling/downwelling areas, where water-sediment matter transport could be intensified by 'seiche pumping'. The results have a wide range of applications including transport of dissolved and suspended matter, assement of shoreline erosion, and exchange processes at the water-sediment boundary. In addition, knowledge of the spatial seiche pattern facilitates estimation of the hypothetical lake response to earthquakes in this seismically active region.

  17. North Fork Watershed Protection Act of 2013

    THOMAS, 113th Congress

    Sen. Baucus, Max [D-MT

    2013-02-07

    09/10/2013 Placed on Senate Legislative Calendar under General Orders. Calendar No. 173. (All Actions) Notes: For further action, see H.R.3979, which became Public Law 113-291 on 12/19/2014. Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  18. North Fork Watershed Protection Act of 2014

    THOMAS, 113th Congress

    Rep. Daines, Steve [R-MT-At Large

    2013-06-05

    03/05/2014 Received in the Senate. Read twice. Placed on Senate Legislative Calendar under General Orders. Calendar No. 314. (All Actions) Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  19. North Fork Watershed Protection Act of 2011

    THOMAS, 112th Congress

    Sen. Baucus, Max [D-MT

    2011-01-31

    05/25/2011 Committee on Energy and Natural Resources Subcommittee on Public Lands and Forests. Hearings held. With printed Hearing: S.Hrg. 112-131. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  20. Barriers impede upstream spawning migration of flathead chub

    USGS Publications Warehouse

    Walters, David M.; Zuellig, Robert E.; Crockett, Harry J.; Bruce, James F.; Lukacs, Paul M.; Fitzpatrick, Ryan M.

    2014-01-01

    Many native cyprinids are declining throughout the North American Great Plains. Some of these species require long reaches of contiguous, flowing riverine habitat for drifting eggs or larvae to develop, and their declining populations have been attributed to habitat fragmentation or barriers (e.g., dams, dewatered channels, and reservoirs) that restrict fish movement. Upstream dispersal is also needed to maintain populations of species with passively drifting eggs or larvae, and prior researchers have suggested that these fishes migrate upstream to spawn. To test this hypothesis, we conducted a mark–recapture study of Flathead Chub Platygobio gracilis within a 91-km reach of continuous riverine habitat in Fountain Creek, Colorado. We measured CPUE, spawning readiness (percent of Flathead Chub expressing milt), and fish movement relative to a channel-spanning dam. Multiple lines of evidence indicate that Flathead Chub migrate upstream to spawn during summer. The CPUE was much higher at the base of the dam than at downstream sites; the seasonal increases in CPUE at the dam closely tracked seasonal increases in spawning readiness, and marked fish moved upstream as far as 33 km during the spawning run. The upstream migration was effectively blocked by the dam. The CPUE of Flathead Chub was much lower upstream of the OHDD than at downstream sites, and <0.2% of fish marked at the dam were recaptured upstream. This study provides the first direct evidence of spawning migration for Flathead Chub and supports the general hypothesis that barriers limit adult dispersal of these and other plains fishes.

  1. Trends in selected water-quality characteristics, Flathead River at Flathead, British Columbia, and at Columbia Falls, Montana, water years, 1975-86

    USGS Publications Warehouse

    Cary, L.E.

    1989-01-01

    Data for selected water quality variables were evaluated for trends at two sampling stations--Flathead River at Flathead, British Columbia (Flathead station) and Flathead River at Columbia Falls, Montana (Columbia Falls station). The results were compared between stations. The analyses included data from water years 1975-86 at the Flathead station and water years 1979-86 at the Columbia Falls station. The seasonal Kendall test was applied to adjusted concentrations for variables related to discharge and to unadjusted concentrations for the remaining variables. Slope estimates were made for variables with significant trends unless data were reported as less than the detection limit. At the Flathead station, concentrations of dissolved solids, calcium, magnesium, sodium, dissolved nitrite plus nitrate nitrogen, ammonia nitrogen (total and dissolved), total organic nitrogen, and total phosphorus increased during the study period. Concentrations of total nitrite plus nitrate nitrogen and dissolved iron decreased during the same period. At the Columbia Falls station, concentrations increased for calcium and magnesium and decreased for sulfate and dissolved phosphorus. No trends were detected for 10 other variables tested at each station. Data for the Flathead station were reanalyzed for water years 1979-86. Trends in the data increased for magnesium and dissolved nitrite plus nitrate nitrogen and decreased for dissolved iron. Magnesium was the only variable that displayed a trend (increasing) at both stations. The increasing trends that were detected probably will not adversely affect the water quality of the Flathead River in the near future. (USGS)

  2. The relationship between land management, fecal indicator bacteria, and the occurrence of Campylobacter and Listeria spp. in water and sediments during synoptic sampling in the S. Fork Broad River Watershed, N.E. Georgia, U.S.A

    NASA Astrophysics Data System (ADS)

    Bradshaw, J. K.; Molina, M.; Sidle, R. C.; Sullivan, K.; Oakley, B.; Berrang, M.; Meinersmann, R.

    2013-12-01

    Fecal indicator bacteria (FIB) and pathogens stored in the bed sediments of streams and rivers may be mobilized into the water column affecting overall water quality. Furthermore, land management may play an important role in the concentrations of FIB and the occurrence of pathogens in stream water and sediments. The purpose of this study was to determine the relationship between FIB and pathogens in stream water and sediment based on three land management-affected categories: agricultural, forest, and waters receiving treated municipal wastewater. Two synoptic sampling events were conducted under baseflow conditions (<0.64 cm of rain within 24h) between October-November, 2012 and May-June, 2013. Counts of the E. coli and E. faecalis and occurrences of the enteric pathogens Campylobacter and Listeria spp. were measured in stream water and sediment samples collected at 15 locations (six agricultural (AG); six forested (FORS); and three receiving discharge from water pollution control plants (WPCP)) in the S. Fork Broad River watershed located in northeast Georgia, USA. Mean E. coli and E. faecalis concentrations were highest in the AG stream water samples (3.08 log MPN 100 mL -1 for E. coli and 3.07 log CFU 100 mL -1 for E. faecalis ) and lowest in the FORS water samples for E. coli (2.37 log MPN 100 mL -1 ) and WPCP water samples for E. faecalis (2.53 log CFU 100 mL -1 ). E. coli concentrations (2.74 log MPN 100 mL -1 ) in the WPCP streams were intermediate. Similar to water samples, E. coli concentrations were highest in the AG sediments (4.31 log MPN g -1 ), intermediate in the WPCP sediments (4.06 log MPN g -1 ), and lowest in the FORS sediments (3.46 log MPN g -1 ). In contrast to E. coli, E. faecalis concentrations were lower (1.10 to 1.31 log CFU g -1 ) and relatively more constant than E. coli in sediments over the three land management categories. Campylobacter was detected in 27% of the water samples and 8% of the sediment samples. The highest occurrence

  3. Lower Flathead System Fisheries Study, South Bay of Flathead Lake, Volume III, 1983-1987 Final Report.

    SciTech Connect

    Cross, David; Waite, Ian

    1988-06-01

    The Lower Flathead System Fisheries Study assessed the effects of Kerr Dam operation on the fisheries of the lower Flathead ecosystem. South Bay, the southern most lobe of Flathead Lake, is the most extensive area of shallow water, and therefore, most effected by changes in lake levels. This study began in January of 1984 and was completed in early 1987. Vegetative and structural cover are relatively limited in South Bay, a condition which could contribute to lower recruitment for some fish species. Our data show that the study area contained 0.04% structural and 5.4% vegetative cover in June at full pool. Both figures are less than 1.0% at minimum pool. Structural complexity mediates the ecological interactions between littoral zone fish and their prey, and can affect local productivity and growth in fish. Structural complexity may also be important to overwinter survival of young perch in Flathead Lake. Winter conditions, including ice cover and fall drawdown, seasonally eliminate the vegetative portion of most rooted macrophytes in South Bay. This results in substantial loss of what little structural cover exists, depriving the perch population of habitat which has been occupied all summer. The loss of cover from draw-down concentrates and probably exposes perch to greater predation, including cannibalism, than would occur if structural complexity were greater. 33 refs., 10 figs., 5 tabs.

  4. EFFECTS OF WATERSHED DISTURBANCE ON SMALL STREAMS

    EPA Science Inventory

    This presentation presents the effects of watershed disturbance on small streams. The South Fork Broad River Watershed was studied to evaluate the use of landscape indicators to predict pollutant loading at small spatial scales and to develop indicators of pollutants. Also studie...

  5. Lower Flathead System Fisheries Study, 1985 Annual Report.

    SciTech Connect

    Pajak, Paul; Bradshaw, William H.; DeSantos, Joseph M.; Darling, James E.

    1986-01-01

    Existing aquatic habitat in the lower Flathead River and its tributaries was assessed for its relationship to the present size, distribution, and maintenance of all salmonid species, northern pike, and largemouth bass populations. The objectives were to assess how and to what extent hydroelectric development and operation affects the quality and quantity of aquatic habitat in the lower Flathead River and its tributaries and life stages of existing trout, pike, and largemouth bass populations, evaluate the potential for increasing quality habitat, and thus game fish production, through mitigation, and develop an array of fisheries management options to mitigate the impacts of present hydroelectric operations, demonstrating under each management option how fish populations would benefit and hydroelectric generation capabilities would be modified.

  6. Watershed modeling and monitoring for assessing nutrient trading viability and increasing the adoption of nutrient management practices

    EPA Science Inventory

    Presentation for the American Water Works Association Water Sustainability Conference. The presentation highlights latest results from water quality trading research conducted by ORD using the East Fork Watershed in Southwestern Ohio as a case study. The watershed has a nutrient ...

  7. Hungry Horse Mitigation; Flathead Lake, 2004-2005 Annual Report.

    SciTech Connect

    Hansen, Barry; Evarts, Les

    2006-06-01

    The Confederated Salish and Kootenai Tribes (CSKT) and Montana Fish Wildlife and Parks (MFWP) wrote the ''Fisheries Mitigation Plan for Losses Attributable to the Construction and Operation of Hungry Horse Dam'' in March 1991 to define the fisheries losses, mitigation alternatives and recommendations to protect, mitigate and enhance resident fish and aquatic habitat affected by Hungry Horse Dam. On November 12, 1991, the Northwest Power Planning Council (NPPC) approved the mitigation plan with minor modifications, called for a detailed implementation plan, and amended measures 903(h)(1) through (7). A long-term mitigation plan was submitted in August 1992, was approved by the Council in 1993, and the first contract for this project was signed on November 11, 1993. The problem this project addresses is the loss of habitat, both in quality and quantity, in the Flathead Lake and River basin resulting from the construction and operation of Hungry Horse Dam. The purpose of the project is to both implement mitigation measures and monitor the biological responses to those measures including those implemented by Project Numbers 9101903 and 9101904. Goals and objectives of the 1994 Fish and Wildlife Program (Section 10.1) addressed by this project are the rebuilding to sustainable levels weak, but recoverable, native populations injured by the hydropower system. The project mitigates the blockage of spawning runs by Hungry Horse Dam by restoring and even creating spawning habitats within direct drainages to Flathead Lake. The project also addresses the altered habitat within Flathead Lake resulting from species shifts and consequent dominance of new species that restricts the potential success of mitigation measures. Specific goals of this project are to create and restore habitat and quantitatively monitor changes in fish populations to verify the efficacy of our mitigation measures. The project consists of three components: monitoring, restoration and research. Monitoring

  8. Hungry Horse Mitigation; Flathead Lake, 2003-2004 Annual Report.

    SciTech Connect

    Hansen, Barry; Evarts, Les

    2005-06-01

    The Confederated Salish and Kootenai Tribes (CSKT) and Montana Fish Wildlife and Parks (MFWP) wrote the ''Fisheries Mitigation Plan for Losses Attributable to the Construction and Operation of Hungry Horse Dam'' in March 1991 to define the fisheries losses, mitigation alternatives and recommendations to protect, mitigate and enhance resident fish and aquatic habitat affected by Hungry Horse Dam. On November 12, 1991, the Northwest Power Planning Council (NPPC) approved the mitigation plan with minor modifications, called for a detailed implementation plan, and amended measures 903(h)(1) through (7). A long-term mitigation plan was submitted in August 1992, was approved by the Council in 1993, and the first contract for this project was signed on November 11, 1993. The problem this project addresses is the loss of habitat, both in quality and quantity, in the Flathead Lake and River basin resulting from the construction and operation of Hungry Horse Dam. The purpose of the project is to both implement mitigation measures and monitor the biological responses to those measures including those implemented by Project Numbers 9101903 and 9101904. Goals and objectives of the 1994 Fish and Wildlife Program (Section 10.1) addressed by this project are the rebuilding to sustainable levels weak, but recoverable, native populations injured by the hydropower system. The project mitigates the blockage of spawning runs by Hungry Horse Dam by restoring and even creating spawning habitats within direct drainages to Flathead Lake. The project also addresses the altered habitat within Flathead Lake resulting from species shifts and consequent dominance of new species that restricts the potential success of mitigation measures. Specific goals of this project are to create and restore habitat and quantitatively monitor changes in fish populations to verify the efficacy of our mitigation measures. The project consists of three components: monitoring, restoration and research. Monitoring

  9. Hungry Horse Mitigation; Flathead Lake, 2001-2002 Annual Report.

    SciTech Connect

    Hansen, Barry

    2003-06-09

    The Confederated Salish and Kootenai Tribes (CSKT) and Montana Fish Wildlife and Parks (MFWP) wrote ''Fisheries Mitigation Plan for Losses Attributable to the Construction and Operation of Hungry Horse Dam'' in March 1991 to define the fisheries losses, mitigation alternatives and recommendations to protect, mitigate and enhance resident fish and aquatic habitat affected by Hungry Horse Dam. On November 12, 1991, the Northwest Power Planning Council (NPPC) approved the mitigation plan with minor modifications, called for a detailed implementation plan, and amended measures 903(h)(1) through (7). A long-term mitigation plan was submitted in August 1992, was approved by the Council in 1993, and the first contract for this project was signed on November 11, 1993. The problem this project addresses is the loss of habitat, both in quality and quantity, in the interconnected Flathead Lake and River basin resulting from the construction and operation of Hungry Horse Dam. The purpose of the project is to both implement mitigation measures and monitor the biological responses to those measures including those implemented by Project Numbers 9101903 and 9101904. Goals and objectives of the 1994 Fish and Wildlife Program (Section 10.1) addressed by this project are the rebuilding to sustainable levels weak, but recoverable, native populations injured by the hydropower system. The project mitigates the blockage of spawning runs by Hungry Horse Dam by restoring and even creating spawning habitats within direct drainages to Flathead Lake. The project also addresses the altered habitat within Flathead Lake resulting from species shifts and consequent dominance of new species that restricts the potential success of mitigation measures. Specific goals of this project are to create and restore habitat and quantitatively monitor changes in fish populations to verify the efficacy of our mitigation measures. The project consists of three components: monitoring, restoration and research

  10. Hungry Horse Mitigation : Flathead Lake : Annual Progress Report 2008.

    SciTech Connect

    Hansen, Barry; Evarts, Les

    2009-08-06

    The Confederated Salish and Kootenai Tribes (CSKT) and Montana Fish Wildlife and Parks (MFWP) wrote the 'Fisheries Mitigation Plan for Losses Attributable to the Construction and Operation of Hungry Horse Dam' in March 1991 to define the fisheries losses, mitigation alternatives and recommendations to protect, mitigate and enhance resident fish and aquatic habitat affected by Hungry Horse Dam. On November 12, 1991, the Northwest Power Planning Council (NPPC) approved the mitigation plan with minor modifications, called for a detailed implementation plan, and amended measures 903(h)(1) through (7). A long-term mitigation plan was submitted in August 1992, was approved by the Council in 1993, and the first contract for this project was signed on November 11, 1993. The problem this project addresses is the loss of habitat, both in quality and quantity, in the Flathead Lake and River basin resulting from the construction and operation of Hungry Horse Dam. The purpose of the project is to both implement mitigation measures and monitor the biological responses to those measures including those implemented by Project Numbers 9101903 and 9101904. Goals and objectives of the 1994 Fish and Wildlife Program (Section 10.1) addressed by this project are the rebuilding to sustainable levels weak, but recoverable, native populations injured by the hydropower system. The project mitigates the blockage of spawning runs by Hungry Horse Dam by restoring and even creating spawning habitats within direct drainages to Flathead Lake. The project also addresses the altered habitat within Flathead Lake resulting from species shifts and consequent dominance of new species that restricts the potential success of mitigation measures. Specific goals of this project are to create and restore habitat and quantitatively monitor changes in fish populations to verify the efficacy of our mitigation measures. The project consists of three components: monitoring, restoration and research. Monitoring

  11. Hungry Horse Mitigation : Flathead Lake : Annual Progress Report 2007.

    SciTech Connect

    Hansen, Barry; Evarts, Les

    2008-12-22

    The Confederated Salish and Kootenai Tribes (CSKT) and Montana Fish Wildlife and Parks (MFWP) wrote the 'Fisheries Mitigation Plan for Losses Attributable to the Construction and Operation of Hungry Horse Dam' in March 1991 to define the fisheries losses, mitigation alternatives and recommendations to protect, mitigate and enhance resident fish and aquatic habitat affected by Hungry Horse Dam. On November 12, 1991, the Northwest Power Planning Council (NPPC) approved the mitigation plan with minor modifications, called for a detailed implementation plan, and amended measures 903(h)(1) through (7). A long-term mitigation plan was submitted in August 1992, was approved by the Council in 1993, and the first contract for this project was signed on November 11, 1993. The problem this project addresses is the loss of habitat, both in quality and quantity, in the Flathead Lake and River basin resulting from the construction and operation of Hungry Horse Dam. The purpose of the project is to both implement mitigation measures and monitor the biological responses to those measures including those implemented by Project Numbers 9101903 and 9101904. Goals and objectives of the 1994 Fish and Wildlife Program (Section 10.1) addressed by this project are the rebuilding to sustainable levels weak, but recoverable, native populations injured by the hydropower system. The project mitigates the blockage of spawning runs by Hungry Horse Dam by restoring and even creating spawning habitats within direct drainages to Flathead Lake. The project also addresses the altered habitat within Flathead Lake resulting from species shifts and consequent dominance of new species that restricts the potential success of mitigation measures. Specific goals of this project are to create and restore habitat and quantitatively monitor changes in fish populations to verify the efficacy of our mitigation measures. The project consists of three components: monitoring, restoration and research. Monitoring

  12. Restore McComas Watershed; Meadow Creek Watershed, 2002-2003 Annual Report.

    SciTech Connect

    McRoberts, Heidi

    2004-01-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. Watershed restoration projects within the Meadow Creek watershed are coordinated with the Nez Perce National Forest. The Nez Perce Tribe began watershed restoration projects within the Meadow Creek watershed of the South Fork Clearwater River in 1996. Progress has been made in restoring the watershed by excluding cattle from critical riparian areas through fencing. During years 2000-2003, trees were planted in riparian areas within the meadow and its tributaries. Culverts have been prioritized for replacement to accommodate fish passage throughout the watershed. Designs for replacement are being coordinated with the Nez Perce National Forest. Twenty miles of road were contracted for decommissioning. Tribal crews completed maintenance to the previously built fence.

  13. 1. View of Clark Fork Vehicle Bridge facing west. Panorama ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Clark Fork Vehicle Bridge facing west. Panorama showing the entire span of bridge from north shore of the Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  14. 3. View of Clark Fork Vehicle Bridge facing southwest. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. View of Clark Fork Vehicle Bridge facing southwest. Bridge from north shore of Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  15. 7. View of Clark Fork Vehicle Bridge facing northwest. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. View of Clark Fork Vehicle Bridge facing northwest. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  16. 4. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shoreof Clark Fork River showing 4 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  17. 2. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  18. Volatile sulfur compounds responsible for an offensive odor of the flat-head, Calliurichthys doryssus.

    PubMed

    Shiomi, K; Noguchi, A; Yamanaka, H; Kikuchi, T; Iida, H

    1982-01-01

    1. The volatiles of a flat-head, Calliurichthys doryssus, which gives out a characteristic offensive odor in living time, were analyzed by gas chromatography. 2. A large quantity of volatile sulfur compounds was detected in the flat-head; of which methyl mercaptan and/or dimethyl disulfide were judged to be responsible for the offensive odor. 3. The contents of methyl mercaptan and dimethyl disulfide were much higher in skin than in muscle and viscera.

  19. EFFECTS OF HABITAT DEGRADATION ON BIOLOGICAL ENDPOINTS IN THE SOUTH FORK BROAD RIVER BASIN, GEORGIA

    EPA Science Inventory

    Many of the streams of the lower Piedmont ecoregion in Georgia have been negatively impacted to some degree by habitat degradation due primarily to sedimentation. The South Fork of the Broad River watershed has been designated as sediment impacted under Section 303(d) of the Clea...

  20. Protect and Restore Mill Creek Watershed : Annual Report CY 2005.

    SciTech Connect

    McRoberts, Heidi

    2006-03-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. The Nez Perce Tribe and the Nez Perce National Forest (NPNF) have formed a partnership in completing watershed restoration activities, and through this partnership, more work is accomplished by sharing funding and resources in our effort. The Nez Perce Tribe began watershed restoration projects within the Mill Creek watershed of the South Fork Clearwater River in 2000. Progress has been made in restoring the watershed through excluding cattle from critical riparian areas through fencing. Starting in FY 2002, continuing into 2004, trees were planted in riparian areas in the meadow of the upper watershed. In addition, a complete inventory of culverts at road-stream crossings was completed. Culverts have been prioritized for replacement to accommodate fish passage throughout the watershed, and one high priority culvert was replaced in 2004. Maintenance to the previously built fence was also completed.

  1. Tuning Forks and Monitor Screens.

    ERIC Educational Resources Information Center

    Harrison, M. A. T.

    2000-01-01

    Defines the vibrations of a tuning fork against a computer monitor screen as a pattern that can illustrate or explain physical concepts like wave vibrations, wave forms, and phase differences. Presents background information and demonstrates the experiment. (Author/YDS)

  2. 77 FR 41332 - Fisheries of the Exclusive Economic Zone Off Alaska; Arrowtooth Flounder, Flathead Sole, Rex Sole...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-13

    ... Economic Zone Off Alaska; Arrowtooth Flounder, Flathead Sole, Rex Sole, Deep-Water Flatfish, and Shallow- Water Flatfish in the Gulf of Alaska Management Area AGENCY: National Marine Fisheries Service (NMFS...: NMFS is prohibiting directed fishing for arrowtooth flounder, flathead sole, rex sole,...

  3. Hungry Horse Dam Fisheries Mitigation; Kokanee Stocking and Monitoring in Flathead Lake, 1996 Progress Report.

    SciTech Connect

    Carty, Daniel; Knoetek, W. Ladd Hansen, Barry

    1997-06-01

    Kokanee salmon Oncorhynchus nerka were introduced into Flathead Lake in 1916. The kokanee population declined in the 1960s and 1970s, and kokanee disappeared from Flathead Lake in the late 1980s. Their disappearance has been attributed to the long-term effects of the construction and operation of Hungry Horse and Kerr dams, excessive harvest by anglers, and changes in the lake food web induced by the introduction of opossum shrimp Mysis relicta. Attempts to reestablish kokanee in the Flathead Lake ecosystem between 1988 and 1991 were unsuccessful. In 1991, Montana Fish, Wildlife and Parks (MFWP) and the Confederated Salish and Kootenai Tribes (CSKT) wrote a mitigation plan to restore kokanee to Flathead Lake. In 1993, MFWP, CSKT, and the U.S. Fish and Wildlife Service wrote a mitigation implementation plan that initiated a 5-year test program to use hatchery-reared fish to reintroduce kokanee to the lake. Stocking hatchery-reared kokanee into Flathead Lake began in 1993; the 5-year {open_quotes}kokanee test{close_quotes} started in 1994 and is scheduled to continue through 1998. The annual stocking objective is 1 million yearling kokanee (6-8 in long). Criteria used to evaluate the success of the 5-year test are (1) 30% survival of kokanee 1 year after stocking, (2) yearling-to-adult survival of 10%, and (3) annual harvest of 50,000 kokanee ({ge} 11 in) and fishing effort {ge} 100,000 angler hours.

  4. Lower Flathead River Fisheries Study, 1983 Annual Report.

    SciTech Connect

    DosSantos, Joseph M.; Darling, James E.; Cross, Paul D.

    1986-07-01

    In January of 1983 a two-phase study of the lower Flathead River was initiated by the Confederated Salish and Kootenai Tribes with funding provided by the Bonneville Power Administration. The study fulfills program measure 804 (a) (3) of the Columbia River Basin Fish and Wildlife Program. During 1983 Phase I of the study was completed resulting in a detailed study plan for the next four years and the methods to be employed during the study. Preliminary observations suggest the present operation of Kerr hydroelectric facility and land use practices within the drainage have combined to significantly reduce spawning success of salmonids and northern pike, and thus recruitment to the fisheries of the main river and tributaries. Main river spawning marshes were observed to be drained frequently during the northern pike spawning season which would result in desiccation of eggs and loss of attached fry. Water level fluctuations also caused trapping of juvenile fish and may be an important source of juvenile mortality.

  5. Evaluation of streamflow records in Flathead River basin, Montana

    USGS Publications Warehouse

    Plunkett, R.T.

    1952-01-01

    This report presents data which are, in general, supplementary to those of the surface-water investigations made in the past by the Geological Survey. Those investigations have consisted essentially of the operation of the many gaging stations on the Flathead River and tributaries. The data presented were obtained from a detailed field investigation of the various manmade devices that are factors influencing the quantity or regimen of the flow at the gaging stations. These factors include diversions from the stream, bypass channels carrying water around the gaging stations, return flow from irrigation or other projects, storage and release of flood waters, and other similar factors. Where feasible, the location, size, effect upon the streamflow, periods of use, method of operation, and similar information are given. The information is segregated into sections corresponding to areas determined by the location of gaging stations. An index of streamflow records is included. A section dealing with the adequacy of .available water-resources data, including location and period of record, also is included. This information is given in general terms only, and is portrayed mainly by maps and graphs.

  6. 22. View of Clark Fork Vehicle Bridge facing downwest side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View of Clark Fork Vehicle Bridge facing down-west side. Looking at road deck and vertical laced channel. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  7. 8. View of Clark Fork Vehicle Bridge facing southwest. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. View of Clark Fork Vehicle Bridge facing southwest. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  8. 11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost span. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  9. 21. View of Clark Fork Vehicle Bridge facing west. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View of Clark Fork Vehicle Bridge facing west. Looking at bridge deck, guard rail, juncture of two bridge spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  10. 12. View of Clark Fork Vehicle Bridge facing south. Approach ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. View of Clark Fork Vehicle Bridge facing south. Approach from the north road. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  11. 18. View of Clark Fork Vehicle Bridge facing north. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of Clark Fork Vehicle Bridge facing north. Looking at north concrete abutment and timber stringers. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  12. 20. View of Clark Fork Vehicle Bridge facing up. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. View of Clark Fork Vehicle Bridge facing up. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  13. 19. View of Clark Fork Vehicle Bridge facing north. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. View of Clark Fork Vehicle Bridge facing north. Looking at north abutment and underside of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  14. Multiple pathways process stalled replication forks.

    PubMed

    Michel, Bénédicte; Grompone, Gianfranco; Florès, Maria-Jose; Bidnenko, Vladimir

    2004-08-31

    Impairment of replication fork progression is a serious threat to living organisms and a potential source of genome instability. Studies in prokaryotes have provided evidence that inactivated replication forks can restart by the reassembly of the replication machinery. Several strategies for the processing of inactivated replication forks before replisome reassembly have been described. Most of these require the action of recombination proteins, with different proteins being implicated, depending on the cause of fork arrest. The action of recombination proteins at blocked forks is not necessarily accompanied by a strand-exchange reaction and may prevent rather than repair fork breakage. These various restart pathways may reflect different structures at stalled forks. We review here the different strategies of fork processing elicited by different kinds of replication impairments in prokaryotes and the variety of roles played by recombination proteins in these processes.

  15. Managing a Watershed Monitoring Project with Innovative Data Telemetry and Communications Software

    EPA Science Inventory

    In collaboration with Clermont County, the U.S. EPA is developing watershed-wide load and transport models to better understand environmental stressors in stream flow and the structure and function of stream ecosystems in the tributaries of the Lower East Fork River. Watershed s...

  16. Managing a Watershed Monitoring Project with Innovative Data Telemetry and Communications Software

    EPA Science Inventory

    In collaboration with Clermont County, the U.S. EPA is developing watershed-wide load and transport models to better understand environmental stressors in stream flow and the structure and function of stream ecosystems in the tributaries of the Lower East Fork River. Watershed se...

  17. Watershed Seasons

    ERIC Educational Resources Information Center

    Endreny, Anna

    2007-01-01

    All schools are located in "watersheds," land that drains into bodies of water. Some watersheds, like the one which encompasses the school discussed in this article, include bodies of water that are walking distance from the school. The watershed cited in this article has a brook and wetland within a several-block walk from the school. This…

  18. 78 FR 65962 - Revision of the Land Management Plan for the Flathead National Forest

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-04

    ... Management Plan which describes the strategic direction for management of forest resources for the next ten... plans. Forest plans describe the strategic direction for management of forest resources for ten to... Forest Service Revision of the Land Management Plan for the Flathead National Forest AGENCY:...

  19. Spatial Variations In The Fate And Transport Of Metals In A Mining-Influenced Stream, North Fork Clear Creek, Colorado

    EPA Science Inventory

    North Fork Clear Creek (NFCC) receives acid-mine drainage (AMD) from multiple abandoned mines in the Clear Creek Watershed. Point sources of AMD originate In the Black Hawk/Central City region of the stream. Water chemistry also is influenced by several non-point sources of AMD,...

  20. Comparison of native and introduced flathead catfish populations in Alabama and Georgia: Growth, mortality, and management

    USGS Publications Warehouse

    Sakaris, P.C.; Irwin, E.R.; Jolley, J.C.; Harrison, D.

    2006-01-01

    We compared growth of flathead catfish Pylodictis olivaris from two native populations in Alabama (Coosa and Tallapoosa rivers) and two introduced populations in Georgia (Ocmulgee and Satilla rivers). We also compared mortality rates and potential outcomes of various management regimes (minimum length limits [MLLs]) among the populations. Total length-log10(age) regression slopes for introduced fish were higher than those for native fish, and von Bertalanffy growth coefficients (K) were greater for introduced fish (Ocmulgee: 0.195; Satilla: 0.201) than for native individuals (Coosa: 0.057; Tallapoosa: 0.059). Therefore, introduced flathead catfish grew more rapidly than those in their native range. Mortality (instantaneous mortality rate, Z) was higher in the Satilla River population (Z = -0.602) than in the Ocmulgee River (Z = -0.227) and Coosa River (Z = -0.156) populations. However, fish in the Satilla River population had been introduced for only 10 years and presumably did not reach their theoretical maximum age, potentially biasing the mortality estimate for that population. Simulation of management regimes in Fishery Analyses and Simulation Tools software predicted that maximum biomass of flathead catfish in the Ocmulgee (1,668 kg) and Satilla (1,137 kg) rivers was substantially larger than that in the Coosa (873 kg) and Tallapoosa (768 kg) populations. However, increased exploitation rates in the Ocmulgee and Satilla River populations resulted in dramatic declines in overall biomass, especially at lower MLLs (254 and 356 mm, respectively). Therefore, in systems where introduced flathead catfish represent an important recreational fishery but have dramatically reduced the abundance of native fishes through predation, minimal protection is recommended. We contend that rapid growth of introduced flathead catfish has major implications for their management and the conservation of native fishes. ?? Copyright by the American Fisheries Society 2006.

  1. Ancient DNA analysis of the extinct North American flat-headed peccary (Platygonus compressus).

    PubMed

    Perry, Tahlia; van Loenen, Ayla L; Heiniger, Holly; Lee, Carol; Gongora, Jaime; Cooper, Alan; Mitchell, Kieren J

    2017-03-28

    The geographical range of extant peccaries extends from the southwestern United States through Central America and into northern Argentina. However, from the Miocene until the Pleistocene now-extinct peccary species inhabited the entirety of North America. Relationships among the living and extinct species have long been contentious. Similarly, how and when peccaries moved from North to South America is unclear. The North American flat-headed peccary (Platygonus compressus) became extinct at the end of the Pleistocene and is one of the most abundant subfossil taxa found in North America, yet despite this extensive fossil record its phylogenetic position has not been resolved. This study is the first to present DNA data from the flat-headed peccary and full mitochondrial genome sequences of all the extant peccary species. We performed a molecular phylogenetic analysis to determine the relationships among ancient and extant peccary species. Our results suggested that the flat-headed peccary is sister-taxon to a clade comprising the extant peccary species. Divergence date estimates from our molecular dating analyses suggest that if extant peccary diversification occurred in South America then their common ancestor must have dispersed from North America to South America well before the establishment of the Isthmus of Panama. We also investigated the genetic diversity of the flat-headed peccary by performing a preliminary population study on specimens from Sheriden Cave, Ohio. Flat-headed peccaries from Sheriden Cave appear to be genetically diverse and show no signature of population decline prior to extinction. Including additional extinct Pleistocene peccary species in future phylogenetic analyses will further clarify peccary evolution.

  2. Dissolved-Solids Load in Henrys Fork Upstream from the Confluence with Antelope Wash, Wyoming, Water Years 1970-2009

    USGS Publications Warehouse

    Foster, Katharine; Kenney, Terry A.

    2010-01-01

    Annual dissolved-solids load at the mouth of Henrys Fork was estimated by using data from U.S. Geological Survey streamflow-gaging station 09229500, Henrys Fork near Manila, Utah. The annual dissolved-solids load for water years 1970-2009 ranged from 18,300 tons in 1977 to 123,300 tons in 1983. Annual streamflows for this period ranged from 14,100 acre-feet in 1977 to 197,500 acre-feet in 1983. The 25-percent trimmed mean dissolved-solids load for water years 1970-2009 was 44,300 tons per year at Henrys Fork near Manila, Utah. Previous simulations using a SPAtially Referenced Regression On Watershed attributes (SPARROW) model for dissolved solids specific to water year 1991 conditions in the Upper Colorado River Basin predicted an annual dissolved-solids load of 25,000 tons for the Henrys Fork Basin upstream from Antelope Wash. On the basis of computed dissolved-solids load data from Henrys Fork near Manila, Utah, together with estimated annual dissolved-solids load from Antelope Wash and Peoples Canal, this prediction was adjusted to 37,200 tons. As determined by simulations with the Upper Colorado River Basin SPARROW model, approximately 56 percent (14,000 tons per year) of the dissolved-solids load at Henrys Fork upstream from Antelope Wash is associated with the 21,500 acres of irrigated agricultural lands in the upper Henrys Fork Basin.

  3. Water-quality, bed-sediment, and biological data (October 2008 through September 2009) and statistical summaries of long-term data for streams in the Clark Fork basin, Montana

    USGS Publications Warehouse

    Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

    2010-01-01

    Water, bed sediment, and biota were sampled in streams from Butte to near Missoula, Montana, as part of a long-term monitoring program in the upper Clark Fork basin; additional water samples were collected in the Clark Fork basin from sites near Missoula downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted by the U.S. Geological Survey in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water samples were collected periodically at 24 sites from October 2008 through September 2009. Bed-sediment and biota samples were collected once at 13 sites during August 2009. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2008 through September 2009. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Turbidity was analyzed for water samples collected at the four sites where seasonal daily values of turbidity were being determined as well as at Clark Fork above Missoula. Nutrients also were analyzed at all the supplemental water-quality sites, except for Clark Fork Bypass, near Bonner. Daily values of suspended-sediment concentration and suspended-sediment discharge were determined for four sites. Bed-sediment data include trace-element concentrations in the fine-grained fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record

  4. Protect and Restore Mill Creek Watershed; Annual Report 2002-2003.

    SciTech Connect

    McRoberts, Heidi

    2004-01-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. Watershed restoration projects within the Mill Creek watershed are coordinated with the Nez Perce National Forest. The Nez Perce Tribe began watershed restoration projects within the Mill Creek watershed of the South Fork Clearwater River in 2000. Progress has been made in restoring the watershed through excluding cattle from critical riparian areas through fencing. During the FY 2002, trees were planted in riparian areas in the meadow of the upper watershed. In addition, a complete inventory of culverts at road-stream crossings was completed. Culverts have been prioritized for replacement to accommodate fish passage throughout the watershed. Maintenance to the previously built fence was also completed.

  5. Replication fork instability and the consequences of fork collisions from rereplication

    PubMed Central

    Alexander, Jessica L.; Orr-Weaver, Terry L.

    2016-01-01

    Replication forks encounter obstacles that must be repaired or bypassed to complete chromosome duplication before cell division. Proteomic analysis of replication forks suggests that the checkpoint and repair machinery travels with unperturbed forks, implying that they are poised to respond to stalling and collapse. However, impaired fork progression still generates aberrations, including repeat copy number instability and chromosome rearrangements. Deregulated origin firing also causes fork instability if a newer fork collides with an older one, generating double-strand breaks (DSBs) and partially rereplicated DNA. Current evidence suggests that multiple mechanisms are used to repair rereplication damage, yet these can have deleterious consequences for genome integrity. PMID:27898391

  6. 5. View of Clark Fork Vehicle Bridge facing east. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View of Clark Fork Vehicle Bridge facing east. Bridge from south shore of Clark Fork River-southernmost span. 1900-era Northern Pacific Railway Bridge in background. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  7. Pathways of mammalian replication fork restart.

    PubMed

    Petermann, Eva; Helleday, Thomas

    2010-10-01

    Single-molecule analyses of DNA replication have greatly advanced our understanding of mammalian replication restart. Several proteins that are not part of the core replication machinery promote the efficient restart of replication forks that have been stalled by replication inhibitors, suggesting that bona fide fork restart pathways exist in mammalian cells. Different models of replication fork restart can be envisaged, based on the involvement of DNA helicases, nucleases, homologous recombination factors and the importance of DNA double-strand break formation.

  8. Effect of variable annual precipitation and nutrient input on nitrogen and phosphorus transport from two Midwestern agricultural watersheds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Precipitation patterns and nutrient inputs impact transport of nitrate (NO3-N) and phosphorus (TP) from Midwest watersheds. Nutrient concentrations and yields from two subsurface-drained watersheds, the Little Cobb River (LCR) in southern Minnesota and the South Fork Iowa River (SFIR) in northern Io...

  9. Evaluation of stomach tubes and gastric lavage for sampling diets from blue catfish and flathead catfish

    USGS Publications Warehouse

    Waters, D.S.; Kwak, T.J.; Arnott, J.B.; Pine, William E.

    2004-01-01

    We compared the ability to extract all stomach contents by using stomach tubes or gastric lavage to sample diets from blue catfish Ictalurus furcatus and flathead catfish Pylodictus olivarus. Pulsed gastric lavage (PGL) removed a significantly greater proportion of stomach content mass (95.6%) from blue catfish than did stomach tubes (14.6%). Percent mass of flathead catfish contents removed with PGL (96.0%) was not significantly different from that removed with stomach tubes (86.9%). Based on the greater effectiveness of PGL for blue catfish, combined with a shorter mean time required per sample (69 versus 118 s) and the better preservation of extracted diet material, we recommend using PGL as a nonlethal technique to collect diet samples from large catfishes.

  10. 2. PLANK COVERED BRANCH FLUME ON THE SOUTH FORK OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. PLANK COVERED BRANCH FLUME ON THE SOUTH FORK OF THE TULE RIVER MIDDLE FORK AND CONCRETE DIVERSION DAM SPILLING WATER. VIEW TO SOUTHEAST. - Tule River Hydroelectric Project, Water Conveyance System, Middle Fork Tule River, Springville, Tulare County, CA

  11. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  12. Protect and Restore Mill Creek Watershed; Annual Report 2004-2005.

    SciTech Connect

    McRoberts, Heidi

    2005-12-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. The Nez Perce Tribe and the Nez Perce National Forest (NPNF) have formed a partnership in completing watershed restoration activities, and through this partnership, more work is accomplished by sharing funding and resources in our effort. The Nez Perce Tribe began watershed restoration projects within the Mill Creek watershed of the South Fork Clearwater River in 2000. Progress has been made in restoring the watershed through excluding cattle from critical riparian areas through fencing. Starting in FY 2002, continuing into 2004, trees were planted in riparian areas in the meadow of the upper watershed. In addition, a complete inventory of culverts at road-stream crossings was completed. Culverts have been prioritized for replacement to accommodate fish passage throughout the watershed, and one high priority culvert was replaced in 2004. Maintenance to the previously built fence was also completed.

  13. Protect and Restore Mill Creek Watershed; Annual Report 2003-2004.

    SciTech Connect

    McRoberts, Heidi

    2004-06-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. The Nez Perce Tribe and the Nez Perce National Forest have formed a partnership in completing watershed restoration activities, and through this partnership, more work is accomplished by sharing funding and resources in our effort. The Nez Perce Tribe began watershed restoration projects within the Mill Creek watershed of the South Fork Clearwater River in 2000. Progress has been made in restoring the watershed through excluding cattle from critical riparian areas through fencing. Starting in FY 2002, continuing into 2004, trees were planted in riparian areas in the meadow of the upper watershed. In addition, a complete inventory of culverts at road-stream crossings was completed. Culverts have been prioritized for replacement to accommodate fish passage throughout the watershed, and designs completed on two of the high priority culverts. Maintenance to the previously built fence was also completed.

  14. Use of benthic invertebrate community structure and the sediment quality triad to evaluate metal-contaminated sediment in the upper Clark Fork River, Montana

    SciTech Connect

    Canfield, T.J.; Kemble, N.E.; Brumbaugh, W.G.; Dwyer, F.J.; Ingersoll, C.G.; Fairchild, J.F. . Midwest Science Center)

    1994-12-01

    The upper Clark Fork River, above Flathead River, is contaminated with large amounts of As, Cd, Cu, Pb, Mn, and Zn ores from past mining activities. The contaminated area extends from the Butte and Anaconda area to at least 230 km downstream to Milltown Reservoir. Both the upper Clark Fork River and Milltown Reservoir have been designated as US Environmental Protection Agency Superfund sites because of metal-contaminated bottom sediments. The authors evaluated the impacts of past mining activities on the Clark Fork River ecosystem using benthic invertebrate community assessment, residue chemistry, and toxicity testing. Oligochaeta and Chironomidae generally accounted for over 90% of the benthic invertebrate community in the soft sediment depositional areas. Taxa of Oligochaeta and Chironomidae were predominantly pollution tolerant. Higher numbers of Chironomidae genera were present at stations with higher concentrations of metals in sediment identified as toxic by the amphipod Hyalella azteca in 28-d exposures. Frequency of mouthpart deformities in genera of Chironomidae was low and did not correspond to concentrations of metals in sediment. Total abundance of organisms/m[sup 2] did not correspond to concentrations of metals in the sediment samples. Chemical analyses, laboratory toxicity tests, and benthic community evaluations all provide evidence of metal-induced degradation to aquatic communities in both the reservoir and the river. Using a weight-of-evidence approach--the Sediment Quality Triad--provided good concurrence among measures of benthic community structure, sediment chemistry, and laboratory toxicity.

  15. Use of benthic invertebrate community structure and the sediment quality triad to evaluate metal-contaminated sediment in the upper Clark Fork River, Montana

    USGS Publications Warehouse

    Canfield, Timothy J.; Kemble, Nile E.; Brumbaugh, William G.; Dwyer, F. James; Ingersoll, Christopher G.; Fairchild, James F.

    1994-01-01

    The upper Clark Fork River, above Flathead River, is contaminated with large amounts of As, Cd, Cu, Pb, Mn, and Zn ores from past mining activities. The contaminated area extends from the Butte and Anaconda area to at least 230 km downstream to Milltown Reservoir. Both the upper Clark Fork River and Milltown Reservoir have been designated as U.S. Environmental Protection Agency Superfund sites because of metal-contaminated bottom sediments. We evaluated the impacts of past mining activities on the Clark Fork River ecosystem using benthic invertebrate community assessment, residue chemistry, and toxicity testing. Oligochaeta and Chironomidae generally accounted for over 90% of the benthic invertebrate community in the soft sediment depositional areas. Taxa of Oligochaeta and Chironomidae were predominantly pollution tolerant. Higher numbers of Chironomidae genera were present at stations with higher concentrations of metals in sediment identified as toxic by the amphipod Hyalella azteca in 28-d exposures. Frequency of mouthpart deformities in genera of Chironomidae was low and did not correspond to concentrations of metals in sediment. Total abundance of organisms/m2 did not correspond to concentrations of metals in the sediment samples. Chemical analyses, laboratory toxicity tests, and benthic community evaluations all provide evidence of metal-induced degradation to aquatic communities in both the reservoir and the river. Using a weight-of-evidence approach-the Sediment Quality Triad - provided good concurrence among measures of benthic community structure, sediment chemistry, and laboratory toxicity.

  16. Watershed modeling and monitoring for assessing nutrient ...

    EPA Pesticide Factsheets

    Presentation for the American Water Works Association Water Sustainability Conference. The presentation highlights latest results from water quality trading research conducted by ORD using the East Fork Watershed in Southwestern Ohio as a case study. The watershed has a nutrient enrichment problem that is creating harmful algal blooms in a reservoir used for drinking water and recreation. Innovative modeling and monitoring is combined to understand how to best manage this water quality problem and costs associated with this endeavor. The presentation will provide an overview of the water quality trading feasibility research. The research includes the development and evaluation of innovative modeling and monitoring approaches to manage watersheds for nutrient pollution using a whole systems approach.

  17. Research reactor fork users manual

    SciTech Connect

    Hsue, S.T.; Menlove, H.O.; Bosler, G.E.; Dye, H.R.; Walton, G.; Halbig, J.K.; Siebelist, R.

    1993-11-01

    This manual describes the design features and operating characteristics of the research reactor fork. The system includes an ion chamber for gross gamma-ray counting, fission chambers for neutron counting, and a collimated high-resolution spectroscopy system for gamma-ray measurements. The neutron and ion chamber measurements are designed to be made underwater in spent-fuel cooling ponds. The neutron and gamma-ray detectors have been designed with high efficiencies to accommodate the relatively low emission rates of neutrons and gamma rays from low-burnup, research-type reactor fuel. This manual presents the design, performance, and test results for the system.

  18. Fifth-wheel fork truck adapter

    NASA Technical Reports Server (NTRS)

    Smith, P. L.

    1969-01-01

    Standard fifth wheel mounted on a rectangular steel structure adapted for use with a fork lift truck provides a fast, safe, and economical way of maneuvering semitrailers in close quarters at plants and warehouses. One operator can move and locate a semitrailer without dismounting from a fork lift truck.

  19. Training Guidelines: Fork Lift Truck Driving.

    ERIC Educational Resources Information Center

    Ceramics, Glass, and Mineral Products Industry Training Board, Harrow (England).

    This manual of operative training guidelines for fork lift truck driving has been developed by the Ceramics, Glass and Mineral Products Industry Training Board (Great Britain) in consultation with a number of firms which manufacture fork lift trucks or which already have training--programs for their use. The purpose of the guidelines is to assist…

  20. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  1. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  2. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  3. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  4. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  5. Hungry Horse Dam Fisheries Mitigation : Fish Passage and Habitat Improvement in the Upper Flathead River Basin, 1991-1996 Progress Report.

    SciTech Connect

    Knotek, W.Ladd; Deleray, Mark; Marotz, Brian L.

    1997-08-01

    In the past 50 years, dramatic changes have occurred in the Flathead Lake and River system. Degradation of fishery resources has been evident, in part due to deterioration of aquatic habitat and introduction of non-endemic fish and invertebrate species. Habitat loss has been attributed to many factors including the construction and operation of Hungry Horse Dam, unsound land use practices, urban development, and other anthropogenic and natural disturbances. Fish migration has also been limited by barriers such as dams and impassible culverts. Cumulatively, these factors have contributed to declines in the distribution and abundance of native fish populations. Recovery of fish populations requires that a watershed approach be developed that incorporates long-term aquatic habitat needs and promotes sound land use practices and cooperation among natural resource management agencies. In this document, the authors (1) describe completed and ongoing habitat improvement and fish passage activities under the Hungry Horse Fisheries Mitigation Program, (2) describe recently identified projects that are in the planning stage, and (3) develop a framework for identifying prioritizing, implementing, and evaluating future fish habitat improvement and passage projects.

  6. Watershed Investigations

    ERIC Educational Resources Information Center

    Bodzin, Alec; Shive, Louise

    2004-01-01

    Investigating local watersheds presents middle school students with authentic opportunities to engage in inquiry and address questions about their immediate environment. Investigation activities promote learning in an investigations interdisciplinary context as students explore relationships among chemical, biological, physical, geological, and…

  7. Assessing the impacts of river regulation on native bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarkii lewisi) habitats in the upper Flathead River, Montana, USA

    USGS Publications Warehouse

    Muhlfeld, Clint C.; Jones, Leslie A.; Kotter, D.; Miller, William J.; Geise, Doran; Tohtz, Joel; Marotz, Brian

    2012-01-01

    Hungry Horse Dam on the South Fork Flathead River, Montana, USA, has modified the natural flow regimen for power generation, flood risk management and flow augmentation for anadromous fish recovery in the Columbia River. Concern over the detrimental effects of dam operations on native resident fishes prompted research to quantify the impacts of alternative flow management strategies on threatened bull trout (Salvelinus confluentus) and westslope cutthroat trout (Oncorhynchus clarkii lewisi) habitats. Seasonal and life‐stage specific habitat suitability criteria were combined with a two‐dimensional hydrodynamic habitat model to assess discharge effects on usable habitats. Telemetry data used to construct seasonal habitat suitability curves revealed that subadult (fish that emigrated from natal streams to the river system) bull trout move to shallow, low‐velocity shoreline areas at night, which are most sensitive to flow fluctuations. Habitat time series analyses comparing the natural flow regimen (predam, 1929–1952) with five postdam flow management strategies (1953–2008) show that the natural flow conditions optimize the critical bull trout habitats and that the current strategy best resembles the natural flow conditions of all postdam periods. Late summer flow augmentation for anadromous fish recovery, however, produces higher discharges than predam conditions, which reduces the availability of usable habitat during this critical growing season. Our results suggest that past flow management policies that created sporadic streamflow fluctuations were likely detrimental to resident salmonids and that natural flow management strategies will likely improve the chances of protecting key ecosystem processes and help to maintain and restore threatened bull trout and westslope cutthroat trout populations in the upper Columbia River Basin.

  8. 23. View of Clark Fork Vehicle Bridge facing upwest side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. View of Clark Fork Vehicle Bridge facing up-west side. Looking at structural connection of top chord, vertical laced channel and diagonal bars. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  9. 13. View of Clark Fork Vehicle Bridge facing south. Concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. View of Clark Fork Vehicle Bridge facing south. Concrete barrier blocks access. Plaque was originally located where strioed traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  10. 14. View of Clark Fork Vehicle Bridge facing north. Approach ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. View of Clark Fork Vehicle Bridge facing north. Approach from the south. Concrete barrier blocks access. Plaque was originally located where striped traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  11. 24. View of one of the plaques from Clark Fork ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. View of one of the plaques from Clark Fork Vehicle Bridge. Presently located at the Bonner County Historical Museum in Sandpoint, Idaho. A plaque was attached at each end of the bridge. Only one remains. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  12. South Fork Holston River basin 1988 biomonitoring

    SciTech Connect

    Saylor, C.F.; Ahlstedt, S.A.

    1990-06-01

    There is concern over the effects of shifts in land use use practices on the aquatic fauna of streams in the South Fork Holston River basin in northwestern North Carolina and southwestern Virginia. Trout reproduction has noticeably declined in the Watauga River subbasin. The Watauga River and Elk River subbasins have been subjected to commercial and resort development. The Middle fork Holston River and the upper South Fork Holston River subbasins have been affected by agricultural and mining activities, respectively (Cox, 1986). To aid reclamation and management of the South Fork Holston basin, Tennessee Valley Authority (TVA) biologists conducted biomonitoring--including index of biotic integrity and macroinvertebrate sampling--on the Middle Fork Holston, South Fork Holston, Watauga, and Elk Rivers to assess cumulative impairment related to changes in habitat and pollutant loading in these subbasins. Biomonitoring can detect environmental degradation, help document problem areas, and assist in development of strategies for managing water quality. This report discusses the methods and materials and results of the biomonitoring of South Fork Holston River Basin. 13 refs., 5 figs., 12 tabs.

  13. Restore McComas Meadows; Meadow Creek Watershed, 2003-2004 Annual Report.

    SciTech Connect

    McRoberts, Heidi

    2006-08-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. Watershed restoration projects within the Meadow Creek watershed are coordinated and cost shared with the Nez Perce National Forest. The Nez Perce Tribe began watershed restoration projects within the Meadow Creek watershed of the South Fork Clearwater River in 1996. Progress has been made in restoring the watershed by excluding cattle from critical riparian areas through fencing, planting trees in riparian areas within the meadow and its tributaries, prioritizing culverts for replacement to accommodate fish passage, and decommissioning roads to reduce sediment input. Designs for culvert replacements are being coordinated with the Nez Perce National Forest. 20 miles of roads were decommissioned. Tribal crews completed maintenance to the previously built fence.

  14. Chromosome identification of the flat-headed cat, Felis planiceps: one more down and five to go.

    PubMed

    Centerwall, W R; Wurster-Hill, D H; Maruska, E J; Theobald, J A

    1977-11-01

    There are 38 recognized feline species in the world. With successful chromosome analysis of the flat-headed cat, Felis planiceps or Prionailurus planiceps, from the secluded river banks and lower mountain slopes of the jungles of Malasia, Borneo, and Sumatra, there remain only 5 rare and endangered species yet unstudied. Although some, including the flat-headed cat, have somewhat distinctive chromosome configurations, all cats thus far karyotyped show 38-chromosome counts, excepting 5 new world "tropicals" which have 36. A pattern of probable feline mutations can be read from the chromosome variations in the different species.

  15. Geology of Glacier National Park and the Flathead Region, Northwestern Montana

    USGS Publications Warehouse

    Ross, Clyde P.

    1959-01-01

    This report summarizes available data on two adjacent and partly overlapping regions in northwestern Montana. The first of these is Glacier National Park plus small areas east and west of the park. The second is here called, for convenience, the Flathead region; it embraces the mountains from the southern tip of Glacier Park to latitude 48 deg north and between the Great Plains on the east and Flathead Valley on the west. The fieldwork under the direction of the writer was done in 1948, 1949, 1950, and 1951, with some work in 1952 and 1953. The two regions together include parts of the Swan, Flathead, Livingstone, and Lewis Ranges. They are drained largely by branches of the Flathead River. On the east and north, however, they are penetrated by tributaries of the Missouri River and in addition by streams that flow into Canada. Roads and highways reach the borders of the regions; but there are few roads in the regions and only two highways cross them. The principal economic value of the assemblage of mountains described in the present report is as a collecting ground for snow to furnish the water used in the surrounding lowlands and as a scenic and wildlife recreation area. A few metallic deposits and lignitic coal beds are known, but these have not proved to be important and cannot, as far as can now be judged, be expected to become so. No oil except minor seeps has yet been found, and most parts of the two regions covered do not appear geologically favorable to the presence of oil in commercial quantities. The high, Hungry Horse Dam on which construction was in progress during the fieldwork now floods part of the Flathead region and will greatly influence the future of that region. The rocks range in age from Precambrian to Recent. The thickest units belong to the Belt series of Precambrian age, and special attention was paid to them. As a result, it is clear that at least the upper part of the series shows marked lateral changes within short distances. This fact

  16. Water-Quality, Bed-Sediment, and Biological Data (October 2005 through September 2006) and Statistical Summaries of Long-Term Data for Streams in the Clark Fork Basin, Montana

    USGS Publications Warehouse

    Dodge, Kent A.; Hornberger, Michelle I.; Dyke, Jessica

    2007-01-01

    Water, bed sediment, and biota were sampled in streams from Butte to below Milltown Reservoir as part of a long-term monitoring program in the upper Clark Fork basin; additional water-quality samples were collected in the Clark Fork basin from sites near Milltown Reservoir downstream to near the confluence of the Clark Fork and Flathead River as part of a supplemental sampling program. The sampling programs were conducted in cooperation with the U.S. Environmental Protection Agency to characterize aquatic resources in the Clark Fork basin of western Montana, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were located on the Clark Fork and selected tributaries. Water-quality samples were collected periodically at 22 sites from October 2005 through September 2006. Bed-sediment and biological samples were collected once at 12 sites during August 2006. This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at all long-term and supplemental monitoring sites from October 2005 through September 2006. Water-quality data include concentrations of selected major ions, trace ele-ments, and suspended sediment. Nutrients also were analyzed in the supplemental water-quality samples. Daily values of suspended-sed-iment concentration and suspended-sediment discharge were determined for four sites, and seasonal daily values of turbidity were determined for four sites. Bed-sediment data include trace-ele-ment concentrations in the fine-grained fraction. Bio-logical data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of long-term water-quality, bed-sediment, and biological data for sites in the upper Clark Fork basin are provided for the period of record since 1985.

  17. Community DECISIONS: stakeholder focused watershed planning.

    PubMed

    Bosch, Darrell; Pease, James; Wolfe, Mary Leigh; Zobel, Christopher; Osorio, Javier; Cobb, Tanya Denckla; Evanylo, Greg

    2012-12-15

    Successful watershed planning can be enhanced by stakeholder involvement in developing and implementing plans that reflect community goals and resource limitations. Community DECISIONS (Community Decision Support for Integrated, On-the-ground Nutrient Reduction Strategies) is a structured decision process to help stakeholders evaluate strategies that reduce watershed nutrient imbalances. A nutrient accounting algorithm and nutrient treatment database provide information on nutrient loadings and costs of alternative strategies to reduce loadings. Stakeholders were asked to formulate goals for the North Fork Shenandoah River Watershed in Virginia and select among strategies to achieve those goals. The Vector Analytic Hierarchy Process was used to rank strategies. Stakeholders preferred a Maximum strategy that included point source upgrades, riparian buffers, no-till corn silage, wheat cover, and bioretention filters in developed areas. Participants generally agreed that the process helped improve communication among stakeholders, was helpful for watershed planning, and should be used for TMDL (Total Maximum Daily Load) planning. Participants suggested more attention be paid to ensuring that all relevant issues are addressed and all information needed to make decisions is available. Watershed planning should provide stakeholders with clear scientific information about physical and socioeconomic processes. However, planning processes must give stakeholders adequate time to consider issues that may not have been addressed by existing scientific models and datasets.

  18. Effects of controlled burning of chaparral on streamflow and sediment characteristics, East Fork Sycamore Creek, central Arizona

    USGS Publications Warehouse

    Baldys, Stanley; Hjalmarson, H.W.

    1994-01-01

    The effects of controlled burning of part of a chaparral-covered drainage basin on streamflow and sediment characteristics were studied in the upper reaches of the Sycamore Creek basin in central Arizona. A paired-watershed method was used to analyze data collected in two phases separated by the controlled burning of 45 percent of the East Fork Sycamore Creek drainage basin by the U.S. Forest Service on October 31, 1981. Statistically significant increases in streamflow in East Fork occurred from October 26, 1982, through August 25, 1984. Streamflow for August 26, 1984, through the end of data collection for the study on May 31, 1986, was generally at or less than preburn levels. An increase in the percentage of time that flow occurred in East Fork was noted for water years 1983 and 1984. No increase in the magnitude of instantaneous peak flows as a result of the burn was discernable at statistically significant levels. Suspended-sediment yields computed for data collected during water year 1983 were significantly greater in the East Fork drainage basin, 546 tons per square mile, than in the West Fork drainage basin, 22.6 tons per square mile. Suspended-sediment yields computed for East Fork and West Fork for water year 1985, 38.3 and 13.3 tons per square mile, respectively, were much closer in yield. These more uniform yields indicate a possible return to preburn conditions. Data collection did not begin until 11 months after the burn; therefore, the largest increases in streamflow and sediment yields, which commonly occur during the year after a burn, may not have been measured. During the second through fourth years after the burn, smaller increases in stream- flow and sediment yields were found in this study than were found in similar studies in this region.

  19. Watershed Science.

    ERIC Educational Resources Information Center

    Green, Tom

    1996-01-01

    Presents activities from an interdisciplinary project studying local watersheds that incorporate a broad spectrum of disciplines including science, math, geography, English, computer science, and political science. Enables students to understand how precipitation changes chemically as it interacts with the soils and human-altered landscape as it…

  20. 75 FR 31321 - Fisheries of the Exclusive Economic Zone Off Alaska; Rock Sole, Flathead Sole, and “Other...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... fisheries data in a timely fashion and would delay the closure of directed fishing for rock sole, flathead... National Oceanic and Atmospheric Administration 50 CFR Part 679 RIN 0648-XW74 Fisheries of the Exclusive... Amendment 80 Limited Access Fishery in Bering Sea and Aleutian Islands Management Area AGENCY:...

  1. 76 FR 77768 - Information Collection; Flathead and McKenzie Rivers and McKenzie National Recreational Trail...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-14

    ... Forest Service Information Collection; Flathead and McKenzie Rivers and McKenzie National Recreational... Visitor Survey and McKenzie River Visitor Survey. DATES: Comments must be received in writing on or before... and McKenzie River Visitor Survey. OMB Number: 0596-NEW. Expiration Date of Approval: NA. Type...

  2. 75 FR 65608 - Flathead National Forest-Swan Lake Ranger District, Montana; Wild Cramer Forest Health and Fuels...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-26

    ... timber, reduce hazardous forest fuels, prescribe burn, and reconstruct and construct permanent roads... before they burn onto private lands; (6) provide forest products to the local timber industry... Forest Service Flathead National Forest--Swan Lake Ranger District, Montana; Wild Cramer Forest...

  3. Winter diel habitat use and movement by subadult bull trout in the upper Flathead River, Montana

    USGS Publications Warehouse

    Muhlfeld, Clint C.; Glutting, Steve; Hunt, Rick; Daniels, Durae; Marotz, Brian

    2003-01-01

    We evaluated the diel habitat use and movement of subadult bull trout Salvelinus confluentus by use of radiotelemetry during winter in the upper Flathead River, Montana. Of the 13 monitored bull trout, 12 (92%) made at least one diel movement to other habitat locations during their respective day–night tracking surveys and moved an average of 73% of the time. The median distance moved from day to night locations by the mobile fish was 86 m (range, 27–594 m). Diel shifts in habitat use by nine of the tagged fish were related to light intensity; nocturnal emergence generally commenced immediately after the onset of night, and daytime concealment occurred at daybreak. When diel shifts in microhabitat use occurred, subadult bull trout moved from deep, midchannel areas during the day to shallow, low-velocity areas along the channel margins without overhead cover at night. Resource managers who wish to protect the overwintering habitat features preferred by subadult bull trout in the upper Flathead River should use natural flow management strategies that maximize and stabilize channel margin habitats at night.

  4. Effects of Water Levels on Productivity of Canada Geese in the Northern Flathead Valley, Final Report.

    SciTech Connect

    Casey, Daniel

    1987-08-01

    The Fish and Wildlife Program of the Northwest Power Planning Council calls for wildlife mitigation at hydroelectric projects in the Columbia River System. Beginning April, 1984, the Bonneville Power Administration funded a study of the effects of the operation of Hungry Horse and Kerr Dams on the western Canada goose (Branta canadensis moffittii) inhabitating the Flathead Valley of northwest Montana. The study was conducted by personnel of the Montana Department of Fish, Wildlife and Parks (MDFWP), to: (1) identify the size and productivity of this population, (2) identify current habitat conditions and losses of nesting and brood-rearing areas, (3) describe the effects of water level fluctuations on nesting and brood-rearing, and (4) identify mitigation alternatives to offset these effects. Annual pair and nest surveys were used to document the location and fate of goose nests. The number of known nesting attempts varied from 44 in 1984 to 108 in 1985, to 136 in 1986 and 134 in 1987. Fifty-four percent of the annual meeting nesting effort took place on elevated sites which were secure from the flooding and dewatering effects of fluctuating water levels. An average of 15 nests were found on stumps in the remnant Flathead River delta, however, an area strongly influenced by the operation of Kerr Dam. Annual nest losses to flooding and predation attributable to fluctuations caused by the dam were recorded. 53 refs., 24 figs., 35 tabs.

  5. Rehabilitate Newsome Creek Watershed, 2007-2008 Annual Report.

    SciTech Connect

    Bransford, Stephanie

    2009-05-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridgetop approach. The Nez Perce Tribe (NPT) and the Nez Perce National Forest (NPNF) have formed a partnership in completing watershed restoration activities, and through this partnership more work is accomplished by sharing funding and resources in our effort. The Nez Perce Tribe began watershed restoration projects within the Newsome Creek watershed of the South Fork Clearwater River in 1997. Progress has been made in restoring the watershed through road decommissioning and culvert replacement. Starting in FY 2001 and continuing into the present, a major stream restoration effort on the mainstem of Newsome Creek has been pursued. From completing a watershed assessment to a feasibility study of 4 miles of mainstem rehabilitation to carrying that forward into NEPA and a final design, we will begin the effort of restoring the mainstem channel of Newsome Creek to provide spawning and rearing habitat for anadromous and resident fish species. Roads have been surveyed and prioritized for removal or improvement as well as culverts being prioritized for replacement to accommodate fish passage throughout the watershed.

  6. Sedimentary Record of syn- and Post-Glacial Climate Change Along the Former LGM ice Terminus, Flathead Lake, Montana

    NASA Astrophysics Data System (ADS)

    Hendrix, M. S.; Hofmann, M.; Moore, J. N.; Sperazza, M.

    2006-12-01

    Located west of the continental divide at the former LGM terminal position of the Flathead Lobe of the Cordilleran Ice Sheet, Flathead Lake (Montana) contains a well preserved record of syn- and post-glacial Quaternary sedimentation. We have studied this record through a combination of geologic mapping around the lake margins, 3.5 kHz and lower frequency seismic reflection profiling of lake sediments, and coring of the lake floor. The oldest part of the Quaternary sedimentary record comprises ice-contact till exposed along the lake basin margins and imaged in deep seismic reflection profiles. Sedimentary facies and geomorphology of the terminal moraine suggest that the Flathead Lobe flowed into a major proglacial lake, probably glacial Lake Missoula. The oldest core sediments recovered from the lake basin consist of a series of clay-rich glacial varves that thin- and fine-upward. These are overlain by a series of anomalously coarse silt beds, each containing a sharp base, upward fining grain size, and lakewide distribution. Depositional age of these beds is constrained as between 14,150±150 cal. Yr BP (14C date on a pine needle below the beds) and 13,180±120 cal. Yr BP (Glacier Peak tephra above the beds). We interpret the silt beds to reflect pulses of sediment delivered to the Flathead Lake basin by high discharge flood events associated with rapid retreat of the Flathead Lobe and possible rapid release of proglacial melt water from upstream tributary valleys dammed by the Flathead Lobe. The transition of Flathead Lake from a proglacial lake to the modern oligotrophic lake system took place shortly after deposition of the Glacier Peak tephra. Interestingly, none of our 8 deep piston cores display an obvious Younger Dryas sedimentologic signal. Holocene core records, combined with information from 3.5 kHz seismic data, indicate periods of significant lake level fluctuation that are likely climate-driven. Of these, the most significant lake drawdown immediately

  7. Channelization and floodplain forests: Impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

    USGS Publications Warehouse

    Oswalt, S.N.; King, S.L.

    2005-01-01

    We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased overbank flooding, a rise in the groundwater table, and ponding of upstream timber. Our objectives were to: (1) determine the composition of floodplain vegetation communities along the degraded river reach, (2) to isolate relationships among these communities, geomorphic features, and environmental variables and (3) evaluate successional changes based on current stand conditions. Vegetation communities were not specifically associated with predefined geomorphic features; nevertheless, hydrologic and geomorphic processes as a result of channelization have clearly affected vegetation communities. The presence of valley plugs and continued degradation of upstream reaches and tributaries on the impacted study reach has arrested recovery of floodplain plant communities. Historically common species like Liquidambar styraciflua L. and Quercus spp. L. were not important, with importance values (IV) less than 1, and occurred in less than 20% of forested plots, while Acer rubrum L., a disturbance-tolerant species, was the most important species on the site (IV = 78.1) and occurred in 87% of forested plots. The results of this study also indicate that channelization impacts on the Middle Fork Forked Deer River are more temporally and spatially complex than previously described for other river systems. Rehabilitation of this system necessitates a long-term, landscape-scale solution that addresses watershed rehabilitation in a spatially and temporally hierarchical manner. ?? 2005 Elsevier B.V. All rights reserved.

  8. DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage.

    PubMed

    Mayle, Ryan; Campbell, Ian M; Beck, Christine R; Yu, Yang; Wilson, Marenda; Shaw, Chad A; Bjergbaek, Lotte; Lupski, James R; Ira, Grzegorz

    2015-08-14

    Most spontaneous DNA double-strand breaks (DSBs) result from replication-fork breakage. Break-induced replication (BIR), a genome rearrangement-prone repair mechanism that requires the Pol32/POLD3 subunit of eukaryotic DNA Polδ, was proposed to repair broken forks, but how genome destabilization is avoided was unknown. We show that broken fork repair initially uses error-prone Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81 endonuclease and a converging fork. Mus81 suppresses template switches between both homologous sequences and diverged human Alu repetitive elements, highlighting its importance for stability of highly repetitive genomes. We propose that lack of a timely converging fork or Mus81 may propel genome instability observed in cancer.

  9. Mus81 and converging forks limit the mutagenicity of replication fork breakage

    PubMed Central

    Mayle, Ryan; Campbell, Ian M.; Beck, Christine R.; Yu, Yang; Wilson, Marenda; Shaw, Chad A.; Bjergbaek, Lotte; Lupski, James R.; Ira, Grzegorz

    2016-01-01

    Most spontaneous DNA double-strand breaks (DSBs) result from replication-fork breakage. Break-induced replication (BIR), a genome rearrangement-prone repair mechanism that requires the Pol32/POLD3 subunit of eukaryotic DNA Polδ, was proposed to repair broken forks, but how genome destabilization is avoided was unknown. We show that broken fork repair initially uses error-prone Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81 endonuclease and a converging fork. Mus81 suppresses template switches between both homologous sequences and diverged human Alu repetitive elements, highlighting its importance for stability of highly repetitive genomes. We propose that lack of a timely converging fork or Mus81 may propel genome instability observed in cancer. PMID:26273056

  10. Longitudinal patterns in flathead catfish relative abundance and length at age within a large river: Effects of an urban gradient

    USGS Publications Warehouse

    Paukert, C.P.; Makinster, A.S.

    2009-01-01

    We investigated the spatial variation of flathead catfish (Pylodictis olivaris) relative abundance and growth in the 274 km long Kansas River to determine if population dynamics of catfish are related to urbanization. Electrofishing was conducted at 462 random sites throughout the river in summer, 2005-2006 to collect fish. Relative abundance of age 1 fish (???200mm), subadult (>200-400mm) and adult fish (>400 mm) ranged from 0.34 to 14.67 fish h-1, mean length at age 1 was 165 (range: 128-195) mm total length (TL) and mean length at age 3 was 376 mm TL (range: 293-419mm TL). The proportion of land use within 200 m of the river edge was between 0 and 0.54 urban. River reaches with high relative abundance of age 1 flathead catfish had high relative abundance of subadult and adult catfish. River reaches with fast flathead catfish growth to age 1 had fast growth to age 3. High urban land use and riprap in the riparian area were evident in river reaches near the heavily populated Kansas City and Topeka, Kansas, USA. Reaches with increased number of log jams and islands had decreased riparian agriculture. Areas of low urbanization had faster flathead catfish growth (r = 0.67, p = 0.005). Relative abundance of flathead catfish was higher in more agricultural areas (r = -0.57, p = 0.02). Changes in land use in riverine environments may alter population dynamics of a fish species within a river. Spatial differences in population dynamics need to be considered when evaluating riverine fish populations. Published in 2008 by John Wiley & Sons Ltd.

  11. RELATIONS BETWEEN LAND USE AND STREAM NUTRIENT CONCENTRATIONS FOR SMALL WATERSHEDS IN THE GEORGIA PIEDMONT

    EPA Science Inventory

    We have been sampling nutrient concentrations in 17 headwater streams within the South Fork Broad River (SFBR) watershed on a monthly basis since November 2001. The streams were classified as either developed (n=4), agriculture/pasture (n=4), mixed land use (n=6) or forested (n=3...

  12. Impacts of Water Level Fluctuations on Kokanee Reproduction in Flathead Lake, 1984 Annual Report.

    SciTech Connect

    Decker-Hess, Janet; Clancey, Patrick

    1984-03-01

    This study was initiated in the fall of 1981 to delineate the extent of successful shoreline spawning of kokanee salmon in Flathead Lake and determine the impacts of the historic and present operations of Kerr and Hungry Horse dams. An investigation of the quantity and quality of groundwater and other factors affecting kokanee reproductive success in Flathead Lake began in the spring of 1982. A total of 719 redds were counted in 17 shoreline areas of Flathead Lake in1983 compared to 592 in 1981 and 1,029 in 1982. Shoreline spawning contributed three percent to the total kokanee spawning in the Flathead drainage in 1983. Fifty-nine percent of the redds were located above 2883 ft, the operational minimum pool. The majority of those redds were constructed between 2885 and 2889 ft. In areas above minimum pool, intergravel dissolved oxygen concentrations were adequate for embryo survival and exhibited a decrease with depth. Limited data indicated apparent velocity may be the key in determining redd distribution. Seventy-five percent of the redds located below minimum pool were constructed in a zone between 2869 and 2883 ft. In individual areas, apparent velocity measurements and intergravel dissolved oxygen concentrations were related to redd density. The variation in intergravel dissolved oxygen concentrations in the Yellow Bay spawning area was partially explained by lake stage fluctuation. As lake stage declined, groundwater apparent velocity increased which increased intergravel dissolved oxygen concentrations. Mean survival to the eyed stage in the three areas below minimum pool was 43 percent. Prior to exposure by lake drawdown, mean survival to the eyed stage in spawning areas above minimum pool was 87 percent. This indicated habitat most conducive to successful embryo survival was in gravels above 2883 ft. prior to significant exposure. Survival in redds exposed to either extended periods of drawdown or to temperatures less than -10% was significantly reduced to

  13. Effects of the Operation of Hungry Horse Dam on the Kokanee Fishery in the Flathead River System, 1983 Annual Progress Report.

    SciTech Connect

    Fraley, John J.

    1983-11-01

    This study was undertaken to assess the effects of the operation of Hungry Horse Dam on the kokanee fishery in the Flathead River system. This annual report covers the 1982-1983 field season concerning the effects of Hungry Horse operations on kokanee abundance, migration, spawning, egg incubation and fry emergence in the Flathead River system. This report also addresses the expected recovery of the mainstem kokanee population under the flow regime recommended by the Department of Fish, Wildlife and Parks in 1982.

  14. Distribution and Movement of Bull Trout in the Upper Jarbidge River Watershed, Nevada

    USGS Publications Warehouse

    Allen, M. Brady; Connolly, Patrick J.; Mesa, Matthew G.; Charrier, Jodi; Dixon, Chris

    2010-01-01

    In 2006 and 2007, we surveyed the occurrence of bull trout (Salvelinus confluentus), the relative distributions of bull trout and redband trout (Oncorhynchus mykiss), and stream habitat conditions in the East and West Forks of the Jarbidge River in northeastern Nevada and southern Idaho. We installed passive integrated transponder (PIT) tag interrogation systems at strategic locations within the watershed, and PIT-tagged bull trout were monitored to evaluate individual fish growth, movement, and the connectivity of bull trout between streams. Robust bull trout populations were found in the upper portions of the East Fork Jarbidge River, the West Fork Jarbidge River, and in the Pine, Jack, Dave, and Fall Creeks. Small numbers of bull trout also were found in Slide and Cougar Creeks. Bull trout were numerically dominant in the upper portions of the East Fork Jarbidge River, and in Fall, Dave, Jack, and Pine Creeks, whereas redband trout were numerically dominant throughout the rest of the watershed. The relative abundance of bull trout was notably higher at altitudes above 2,100 m. This study was successful in documenting bull trout population connectivity within the West Fork Jarbidge River, particularly between West Fork Jarbidge River and Pine Creek. Downstream movement of bull trout to the confluence of the East Fork and West Fork Jarbidge River both from Jack Creek (rkm 16.6) in the West Fork Jarbidge River and from Dave Creek (rkm 7.5) in the East Fork Jarbidge River was detected. Although bull trout exhibited some downstream movement during the spring and summer, much of their emigration occurred in the autumn, concurrent with decreasing water temperatures and slightly increasing flows. The bull trout that emigrated were mostly age-2 or older, but some age-1 fish also emigrated. Upstream movement by bull trout was detected less than downstream movement. The overall mean annual growth rate of bull trout in the East Fork and West Fork Jarbidge River was 36 mm

  15. Watersheds: Where We Live.

    ERIC Educational Resources Information Center

    Vandas, Steve

    1997-01-01

    Presents information about watersheds including water quantity, water quality, floods and floodplains. Lists resources for learning more about watersheds as well as Internet resources. Includes a foldout that can be used to teach children about watersheds and floodplains. (JRH)

  16. 5. Roaring Fork Motor Nature Trail, vista at stop three. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  17. 2. Roaring Fork Motor Nature Trail, road view before first ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  18. 1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. 7. Roaring Fork Motor Nature Trail, rocks along edge of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  20. 3. Roaring Fork Motor Nature Trail, view between second and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  1. 11. Roaring Fork Motor Nature Trail, boulders along road after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  2. Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  3. 9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  4. 6. Roaring Fork Motor Nature Trail, road view after stop ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  5. 12. Roaring Fork Motor Nature Trail, place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  6. 12. PLANK BRIDGE ON OLD ROAD NEAR NORTH FORK VIRGIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. PLANK BRIDGE ON OLD ROAD NEAR NORTH FORK VIRGIN RIVER BRIDGE, FACING EAST - Zion-Mount Carmel Highway, Virgin River Bridge, Spanning North Fork of Virgin River on Zion-Mount Carmel Highway, Springdale, Washington County, UT

  7. South Fork Latrine showing north and west sides, general view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine showing north and west sides, general view to southeast - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  8. South Fork Latrine, east elevation showing structure in context, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, east elevation showing structure in context, view west - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  9. South Fork Latrine, oblique view showing south and east sides; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, oblique view showing south and east sides; view northwest - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  10. 15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO SETTLING BASIN, SHOWING RIGHT FORK WITH GATE IN PLACE AND A FEW NEEDLES IN PLACE - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

  11. 8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  12. Resonant tuning fork detector for electromagnetic radiation.

    PubMed

    Pohlkötter, Andreas; Willer, Ulrike; Bauer, Christoph; Schade, Wolfgang

    2009-02-01

    A mechanical quartz microresonator (tuning fork) is used to detect electromagnetic radiation. The detection scheme is based on forces created due to the incident electromagnetic radiation on the piezoelectric tuning fork. A force can be created due to the transfer of the photon momentum of the incident electromagnetic radiation. If the surfaces of the tuning fork are nonuniformly heated, a second force acts on it, the so-called photophoretic force. These processes occur for all wavelengths of the incident radiation, making the detector suitable for sensing of ultraviolet, visible, and mid-infrared light, even THz-radiation. Here the detector is characterized in the visible range; noise analysis is performed for 650 nm and 5.26 microm. A linear power characteristic and the dependence on pulse lengths of the incoming light are shown. Examples for applications for the visible and mid-infrared spectral region are given by 2f and absorption spectroscopy of oxygen and nitric oxide, respectively.

  13. Modelling the Species Distribution of Flat-Headed Cats (Prionailurus planiceps), an Endangered South-East Asian Small Felid

    PubMed Central

    Hearn, Andrew J.; Hesse, Deike; Mohamed, Azlan; Traeholdt, Carl; Cheyne, Susan M.; Sunarto, Sunarto; Jayasilan, Mohd-Azlan; Ross, Joanna; Shapiro, Aurélie C.; Sebastian, Anthony; Dech, Stefan; Breitenmoser, Christine; Sanderson, Jim; Duckworth, J. W.; Hofer, Heribert

    2010-01-01

    Background The flat-headed cat (Prionailurus planiceps) is one of the world's least known, highly threatened felids with a distribution restricted to tropical lowland rainforests in Peninsular Thailand/Malaysia, Borneo and Sumatra. Throughout its geographic range large-scale anthropogenic transformation processes, including the pollution of fresh-water river systems and landscape fragmentation, raise concerns regarding its conservation status. Despite an increasing number of camera-trapping field surveys for carnivores in South-East Asia during the past two decades, few of these studies recorded the flat-headed cat. Methodology/Principal Findings In this study, we designed a predictive species distribution model using the Maximum Entropy (MaxEnt) algorithm to reassess the potential current distribution and conservation status of the flat-headed cat. Eighty-eight independent species occurrence records were gathered from field surveys, literature records, and museum collections. These current and historical records were analysed in relation to bioclimatic variables (WorldClim), altitude (SRTM) and minimum distance to larger water resources (Digital Chart of the World). Distance to water was identified as the key predictor for the occurrence of flat-headed cats (>50% explanation). In addition, we used different land cover maps (GLC2000, GlobCover and SarVision LLC for Borneo), information on protected areas and regional human population density data to extract suitable habitats from the potential distribution predicted by the MaxEnt model. Between 54% and 68% of suitable habitat has already been converted to unsuitable land cover types (e.g. croplands, plantations), and only between 10% and 20% of suitable land cover is categorised as fully protected according to the IUCN criteria. The remaining habitats are highly fragmented and only a few larger forest patches remain. Conclusion/Significance Based on our findings, we recommend that future conservation efforts for

  14. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  15. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  16. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  17. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  18. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  19. Impacts of Water Level Fluctuations on Kokanee Reproduction in Flathead Lake, 1985 Annual Report.

    SciTech Connect

    Beattie, Will; Fraley, John J.; Decker-Hess, Janet

    1986-06-01

    This study has investigated the effects of the operation of Kerr Dam on the reproductive success of kokanee that spawn along the shores of Flathead Lake. We have estimated the spawning escapement to the lakeshore, characterized spawning habitat, monitored egg and alevin survival in redds, and related survival to length of redd exposure due to lake drawdown. Groundwater discharge apparently attracts kokanee to spawning sites along the lakeshore and is responsible for prolonging egg survival in redds above minimum pool. We have quantified and described the effect of lake drawdown on groundwater flux in spawning areas. This report defines optimal lakeshore spawning habitat and discusses eqg and alevin survival both in and below the varial zone.

  20. Dispersal, Mating Events and Fine-Scale Genetic Structure in the Lesser Flat-Headed Bats

    PubMed Central

    Guo, Tingting; Flanders, Jon; Zhang, Shuyi

    2013-01-01

    Population genetic structure has important consequences in evolutionary processes and conservation genetics in animals. Fine-scale population genetic structure depends on the pattern of landscape, the permanent movement of individuals, and the dispersal of their genes during temporary mating events. The lesser flat-headed bat (Tylonycteris pachypus) is a nonmigratory Asian bat species that roosts in small groups within the internodes of bamboo stems and the habitats are fragmented. Our previous parentage analyses revealed considerable extra-group mating in this species. To assess the spatial limits and sex-biased nature of gene flow in the same population, we used 20 microsatellite loci and mtDNA sequencing of the ND2 gene to quantify genetic structure among 54 groups of adult flat-headed bats, at nine localities in South China. AMOVA and FST estimates revealed significant genetic differentiation among localities. Alternatively, the pairwise FST values among roosting groups appeared to be related to the incidence of associated extra-group breeding, suggesting the impact of mating events on fine-scale genetic structure. Global spatial autocorrelation analyses showed positive genetic correlation for up to 3 km, indicating the role of fragmented habitat and the specialized social organization as a barrier in the movement of individuals among bamboo forests. The male-biased dispersal pattern resulted in weaker spatial genetic structure between localities among males than among females, and fine-scale analyses supported that relatedness levels within internodes were higher among females than among males. Finally, only females were more related to their same sex roost mates than to individuals from neighbouring roosts, suggestive of natal philopatry in females. PMID:23349888

  1. Grand Forks - East Grand Forks Urban Water Resources Study. Social and Environmental Inventory.

    DTIC Science & Technology

    1979-01-01

    Utilities 107-108 FIGUR~ES Figure Number Title Pag 14 Projected Population - Region 67 1 5 Projected Population - Grand Forks County 68 Study Area 16...Projected Population - Polk County Study Area 68 17 Planning Regions 105 TABLES Tale Number Title Pg 21 Historic Sites in the Grand Forks Area 61I 22...of Indians, traders and settlers; and those related to the populations which currently reside in the area. The information on archeology and history

  2. Twelve Years of Monitoring Phosphorus and Suspended-Solids Concentrations and Yields in the North Fork Ninnescah River above Cheney Reservoir, South-Central Kansas 1997-2008

    USGS Publications Warehouse

    Stone, Mandy L.; Graham, Jennifer L.; Ziegler, Andrew C.

    2009-01-01

    Cheney Reservoir, located on the North Fork Ninnescah River in south-central Kansas, is the primary water supply for the city of Wichita and an important recreational resource. Concerns about taste-and-odor occurrences in Cheney Reservoir have drawn attention to potential pollutants, including total phosphorus (TP) and total suspended solids (TSS). July 2009 was the 15th anniversary of the establishment of the Cheney Reservoir Watershed pollution management plan. The U.S. Geological Survey (USGS), in cooperation with the city of Wichita, has collected water-quality data in the basin since 1996, and has monitored water quality continuously on the North Fork Ninnescah River since 1998. This fact sheet describes 12 years (1997-2008) of computed TP and TSS data and compares these data with water-quality goals for the North Fork Ninnescah River, the main tributary to Cheney Reservoir.

  3. Homologous recombination as a replication fork escort: fork-protection and recovery.

    PubMed

    Costes, Audrey; Lambert, Sarah A E

    2012-12-27

    Homologous recombination is a universal mechanism that allows DNA repair and ensures the efficiency of DNA replication. The substrate initiating the process of homologous recombination is a single-stranded DNA that promotes a strand exchange reaction resulting in a genetic exchange that promotes genetic diversity and DNA repair. The molecular mechanisms by which homologous recombination repairs a double-strand break have been extensively studied and are now well characterized. However, the mechanisms by which homologous recombination contribute to DNA replication in eukaryotes remains poorly understood. Studies in bacteria have identified multiple roles for the machinery of homologous recombination at replication forks. Here, we review our understanding of the molecular pathways involving the homologous recombination machinery to support the robustness of DNA replication. In addition to its role in fork-recovery and in rebuilding a functional replication fork apparatus, homologous recombination may also act as a fork-protection mechanism. We discuss that some of the fork-escort functions of homologous recombination might be achieved by loading of the recombination machinery at inactivated forks without a need for a strand exchange step; as well as the consequence of such a model for the stability of eukaryotic genomes.

  4. Simulating effects of land use policies on extent of the wildland urban interface and wildfire risk in Flathead County, Montana.

    PubMed

    Paveglio, Travis B; Prato, Tony; Hardy, Michael

    2013-11-30

    This study used a wildfire loss simulation model to evaluate how different land use policies are likely to influence wildfire risk in the wildland urban interface (WUI) for Flathead County, Montana. The model accounts for the complex socio-ecological interactions among climate change, economic growth, land use change and policy, homeowner mitigations, and forest treatments in Flathead County's WUI over the five 10-year subperiods comprising the future evaluation period (i.e., 2010-2059). Wildfire risk, defined as expected residential losses from wildfire [E(RLW)], depends on the number of residential properties on parcels, the probability that parcels burn, the probability of wildfire losses to residential structures on properties given the parcels on which those properties are located burn, the average percentage of wildfire-related losses in aesthetic values of residential properties, and the total value (structures plus land) of residential properties. E(RLW) for the five subperiods is simulated for 2010 (referred to as the current), moderately restrictive, and highly restrictive land use policy scenarios, a moderate economic growth scenario and the A2 greenhouse gas emissions scenario. Results demonstrate that increasingly restrictive land use policy for Flathead County significantly reduces the amount and footprint of future residential development in the WUI. In addition, shifting from the current to a moderately restrictive land use policy for Flathead County significantly reduces wildfire risk for the WUI, but shifting from the current to a highly restrictive land use policy does not significantly reduce wildfire risk in the WUI. Both the methods and results of the study can help land and wildfire managers to better manage future wildfire risk and identify residential areas having potentially high wildfire risk.

  5. Determination of Fishery Losses in the Flathead System Resulting from the Construction of Hungry Horse Dam, 1986 Final Completion Report.

    SciTech Connect

    Zubik, Raymond J.; Fraley, John

    1987-01-01

    This study is part of the Northwest Power Planning Council's residential fish and wildlife plan, which is responsible for mitigating damages to fish and wildlife resources caused by hydroelectric development in the Columbia River basin. The major goal of this study was to provide estimates of fishery losses to the Flathead system as a result of the completion of Hungry Horse Dam and to propose mitigation alternatives for enhancing the fishery. Construction of Hungry Horse Dam had the greatest adverse impacts on cutthroat and full trout from Flathead Lake and mitigative measures should be taken to offset these losses, if biologically and economically feasible. Also, other losses to fish and wildlife have been documented in the Flathead basin due to hydroelectric facilities and their operation. Some of these research projects will not be completed until 1989, when mitigation will be recommended using a basin-wide approach. Since HHR is at the headwaters of the Columbia system, mitigative measures may also affect downstream projects. Therefore, we presented an array of possible mitigation alternatives for consideration by decision-makers, with suggestions on the ones we feel are the most cost effective. Possible mitigation measures are included.

  6. Kokanee Stocking and Monitoring, Flathead Lake, 1993-1994 Annual Report.

    SciTech Connect

    Deleray, Mark; Fredenberg, Wade; Hansen, Barry

    1995-07-01

    One mitigation goal of the Hungry Horse Dam fisheries mitigation program, funded by the Bonneville Power Administration, is to replace lost production of 100,000 adult kokanee in Flathead Lake. The mitigation program calls for a five-year test to determine if kokanee can be reestablished in Flathead Lake. The test consists. of annual stocking of one million hatchery-raised yearling kokanee. There are three benchmarks for judging the success of the kokanee reintroduction effort: (1) Post-stocking survival of 30 percent of planted kokanee one year after stocking; (2) Yearling to adult survival of 10 percent (100,000 adult salmon); (3) Annual kokanee harvest of 50,000 or more fish per year by 1998, with an average length of 11 inches or longer for harvested fish, and fishing pressure of 100,000 angler hours or more. Kokanee were the primary sport fish species in the Flathead Lake fishery in the early 1900s, and up until the late 1980s when the population rapidly declined in numbers and then disappeared. Factors identified which influenced the decline of kokanee are the introduction of opossum shrimp (Mysis relicta), hydroelectric operations, overharvest through angling, and competition and/or predation by lake trout (Salvelinus namaycush) and lake whitefish (Coregonur clupeaformis). The purpose of this report was to summarize the stocking program and present monitoring results from the 1993 and 1994 field seasons. In June 1993, roughly 210,000 yearling kokanee were stocked into two bays on the east shore of Flathead Lake. Following stocking, we observed a high incidence of stocked kokanee in stomach samples from lake trout captured in areas adjacent to the stocking sites and a high percentage of captured lake trout containing kokanee. Subsequent monitoring concluded that excessive lake trout predation precluded significant survival of kokanee stocked in 1993. In June 1994, over 802,000 kokanee were stocked into Big Arm Bay. The combination of near optimum water

  7. Effects of the Operation of Hungry Horse Dam on the Kokanee Fishery in the Flathead River System, 1984 Annual Progress Report.

    SciTech Connect

    Fraley, John J.

    1984-12-01

    This study assessed the effects of the operation of Hungry Horse Dam on the kokanee fishery in the Flathead River system. This report covers the 1983-84 field season concerning the effects of Hungry Horse operations on kokanee abundance and reproductive success in the upper Flathead River system. This report also addresses the projected recovery of the main stem kokanee run under the flow regime recommended by the Department of Fish, Wildlife and Parks and implemented by the Bureau of Reclamation and Bonneville Power Administration in 1982. An estimated 58,775 kokanee reached spawning grounds in the Flathead River System in 1983. The 1983 spawning run was composed of 92% age III + fish, as compared to an average of 80% from 1972-1983. A total of 6883 kokanee redds were enumerated in the main stem Flathead River in 1983. A total of 2366 man-days of angling pressure was estimated during the 1983 kokanee lure fishery in the Flathead River system. Estimated numbers of fry emigrating from McDonald Creek, the Whitefish River and Brenneman's Slough were 13,100,000, 66,254 and 37,198, yielding egg to fry survival rates of 76%, 10.4% and 19.2%.

  8. Henneguya laseeae n. sp. from flathead catfish (Pylodictis olivaris) in the upper Mississippi River.

    PubMed

    Leis, Eric M; Rosser, Thomas G; Baumgartner, Wes A; Griffin, Matt J

    2017-01-01

    A novel species of Henneguya was isolated from flathead catfish (Pylodictis olivaris) captured in the upper Mississippi River near Lansing (Allamakee County), IA, and La Crosse (La Crosse County), WI. Designated Henneguya laseeae n. sp., this novel species is described using critical morphological features, histology, and 18S ribosomal RNA gene sequence. Ovoid cysts, ranging from 1200 to 1800 μm in width, tended to be at filament tips or in the distal third, often directly on the filament midline, but occasionally paramedian. Lanceolate-shaped myxospores were consistent with those of the genus Henneguya. The spore body was 16.2 ± 0.5 μm (mean ± standard deviation; range = 15.1-17.0 μm) in length, 6.0 ± 0.4 μm (5.1-6.6 μm) in width, and 4.7 ± 0.2 μm (4.4-4.9 μm) thick. The two polar capsules at the anterior of the spore body were 5.9 ± 0.3 μm (5.3-6.3 μm) in length and 1.8 ± 0.1 μm (1.6-2.1 μm) in width and contained six to seven turns in the polar filament. The caudal processes tapered to fine points and were 54.3 ± 2.9 μm (49.1-61.7 μm) in length. Total spore length was 70.4 ± 3.3 μm (64.5-79.4 μm). The spores and plasmodium of this species are of similar size and morphology to other species of Henneguya from ictalurid fishes. Additionally, the 18S rRNA gene sequences placed this isolate within a clade populated by Henneguya spp. from North American ictalurids. This is the first reported species of Henneguya from flathead catfish.

  9. Hydro power benefits of cooperative watershed management

    SciTech Connect

    Harrison, L.L.; Lindquist, D.S.

    1995-12-31

    This paper describes the efforts of Pacific Gas and Electric Company (PG&E) in cooperation with a number of agencies and public and private land managers to reduce erosion and restore the health of the East Branch North Fork Feather River (EBNFFR) watershed in Plumas County, California. Erosion of the 2600 square kilometer watershed has been identified as a major contributor of sediments to PG&E`s Rock Creek and Cresta hydroelectric reservoirs which have collected more than 5.4 million cubic meters of sediment over the past 45 years. PG&E and the 17 other participants of the cooperative erosion control program are joined by a {open_quotes}Memorandum of Agreement{close_quotes} (MOA) and are applying {open_quotes}Coordinated Resource Management{close_quotes} (CRM). To date, more than 33 individual watershed improvement projects and a comprehensive erosion control strategy document have been completed. It is anticipated that over the long term, the erosion control program may reduce the water-borne sediment delivery to Rock Creek and Cresta reservoirs by as much as 50 percent. PG&E benefits from the program through reduced sediment deposition in the reservoirs, reduced sediment wear on the power turbines, and potential increases in base flow during summer months when water power is of greatest value.

  10. Bedload measurements, East Fork River, Wyoming.

    PubMed

    Leopold, L B; Emmett, W W

    1976-04-01

    A bedload trap in the riverbed provided direct quantitative measurement of debris-transport rate in the East Fork River, Wyoming, a basin of 466 km(2) drainage area. Traction load moves only during the spring snow melt season. Data collected in three spring runoff seasons during which a peak flow of 45 m(3)/s occurred showed that transport rate is correlated with power expenditure of the flowing water and at high flows becomes directly proportional to power as suggested by Bagnold.

  11. Environmental Inventory: Little South Fork Cumberland River.

    DTIC Science & Technology

    1981-11-01

    28 2.2.4.1 Paleo-Indian Tradition ---------- 28 2.2.4.2 Archaic Tradition --------------- 29 2.2.4.3 Woodland Tradition...Soils as Woodland ----------- 128 3.7.4.1 Woodland Suitability Grouping of Soils ------------- 129 3.7.5 Use of the Soils for Wildlife ---------- 133...Stations on the Little South Fork Cumberland River in June and September 1978 and July 1979 - 94 12. Woodland Management Interpretations by Woodland

  12. North Fork Snoqualmie River Basin Wildlife Study.

    DTIC Science & Technology

    1981-03-01

    more recent origin, never having been a natural lake. 118 Shoreline materials arv still in the process of washing into the depths of the South Fork...10-12 Twin Peaks - Philippa Lake 9 12-24 Bessemer Mountain 13 Total 52-55 44-64 , Estimates provided by John Cook (Olympic Taxidermy, Renton, Wash...organisms which use resources efficient- ly. This process results in wildlife populations which are balanced with their environments. When resources

  13. Examining Suitable Soil Regimes for Reestablishment of Camassia Quamash (Blue Camas), Flathead Indian Reservation

    NASA Astrophysics Data System (ADS)

    Bald, A. M.; Davis, J. M.

    2014-12-01

    Soils are the foundation of all biotic communities and play a substantial role in facilitating the uptake of water and nutrients in many terrestrial plants. Plants can grow to their potential only if the soil supports an environment conducive to growth. Soil chemical composition and texture directly influence the rate of water and nutrient ion uptake in vegetation. Prairie Wetlands have experienced the most dramatic land use changes within the United States throughout the last century. Soils deteriorate from erosion, compaction, use of pesticides, herbicides, and fertilizers associated with agriculture and urbanization. Transitioning soil regimes in the US have been the impetus for numerous restoration activities that attempt to protect or remediate loss to native or functional plant groups. Success of plant restoration efforts is dependent on knowledge about regional soil regimes. Camassia Quamash (Blue Camas), an ephemeral wetland bulbaceous herb is a culturally significant edible plant to the Pacific Northwest tribes and was only surpassed as a subsistence trade commodity by Salmon. The literature about camas and suitable soil types for it to grow is limited. The Confederated Salish and Kootenai Tribes interest in restoring the plant to the Flathead Indian Reservation (FIR) prompted a series of research initiatives to document baseline parameters of remaining camas stands. Baseline soil conditions examining chemical regimes and soil textures on four FIR observed camas sites were analyzed. Samples indicated that remaining camas stands occurred in loamy nutrient rich prairie wetland to lightly forested soil regimes.

  14. Identification of the sex-determining region in flathead grey mullet (Mugil cephalus).

    PubMed

    Dor, L; Shirak, A; Rosenfeld, H; Ashkenazi, I M; Band, M R; Korol, A; Ronin, Y; Seroussi, E; Weller, J I; Ron, M

    2016-12-01

    Elucidation of the sex-determination mechanism in flathead grey mullet (Mugil cephalus) is required to exploit its economic potential by production of genetically determined monosex populations and application of hormonal treatment to parents rather than to the marketed progeny. Our objective was to construct a first-generation linkage map of the M. cephalus in order to identify the sex-determining region and sex-determination system. Deep-sequencing data of a single male was assembled and aligned to the genome of Nile tilapia (Oreochromis niloticus). A total 245 M. cephalus microsatellite markers were designed, spanning the syntenic tilapia genome assembly at intervals of 10 Mb. In the mapping family of full-sib progeny, 156 segregating markers were used to construct a first-generation linkage map of 24 linkage groups (LGs), corresponding to the number of chromosomes. The linkage map spanned approximately 1200 cM with an average inter-marker distance of 10.6 cM. Markers segregating on LG9 in two independent mapping families showed nearly complete concordance with gender (R(2)  = 0.95). The sex determining locus was fine mapped within an interval of 8.6 cM on LG9. The sex of offspring was determined only by the alleles transmitted from the father, thus indicating an XY sex-determination system.

  15. Prevalence and transmission of cercariae causing schistosome dermatitis in Flathead Lake, Montana.

    PubMed

    Loken, B R; Spencer, C N; Granath, W O

    1995-08-01

    Numerous studies have been conducted on swimmer's itch, but very few have been in Montana and none on Flathead Lake, the largest, natural freshwater lake in the western United States. We conducted a study to determine the prevalence of hosts transmitting cercariae causing swimmer's itch in this lake. Hosts for this life cycle were determined by direct observation of fresh waterfowl fecal material for the presence of miracidia, and snails for the presence of cercariae. Swimmer's itch-producing cercariae were verified directly by placing various species on the arms of human volunteers and waiting for a reaction. Results of the study were further substantiated using a controlled experiment in which snails were individually infected with miracidia from the suspected waterfowl host and then checked for infection after a 6-wk incubation period. Our findings show that the common merganser (Mergus merganser) and the snail Stagnicola elrodi were natural hosts of the swimmer's itch parasite (Trichobiharzia ocellata) with prevalences of 84% and 2.0%, respectively. To our knowledge this is the first documented case of S. elrodi transmitting the swimmer's itch cercariae.

  16. Needs assessment for the Greenway Grand Forks-East Grand Forks development and public education

    NASA Astrophysics Data System (ADS)

    Munski, Laura

    Following the flood of 1997, the U.S. Army Corps of Engineers included the Greenway Grand Forks---East Grand Forks (the Greenway) as a flood control measure for Grand Forks, North Dakota and East Grand Forks, Minnesota. It extends along both the Red River of the North and the Red Lake River, encompassing 2200 acres of land. The cities of Grand Forks and East Grand Forks hired consultants to assist with the postflood planning process. The planning process culminated with the Red River of the North Greenway Final Report (Flink, 1998). The purpose of this study was to determine if the development of the Greenway addressed the objectives of the planning report. The history of the land adjacent to the rivers was reviewed to document the progression of riverfront development. Anecdotal evidence was collected, field observations were made, city council minutes were reviewed, Greenway Technical Committee members were interviewed, Greenway Technical Committee minutes were reviewed, and the Greenway Grand Forks---East Grand Forks survey results were reviewed to determine if the objectives of the Red River of the North Greenway Final Report were addressed. A cross section survey was designed by Laura Munski for this dissertation research. The survey was approved by the Greenway Technical Committee. The survey collected both quantitative and qualitative data from the community. The purpose of the survey portion of the research project was to ascertain how residents were kept informed of activities on the Greenway and what amenities residents were using on the Greenway and to solicit their comments regarding the Greenway. The results of the survey research were used in both marketing and event planning for the Greenway. The singular qualitative survey question gave respondents an opportunity to share their comments regarding the Greenway. The qualitative data analysis provided insight to the amenities and educational programs desired by respondents, their concerns regarding the

  17. Managing Watersheds with WMOST

    EPA Pesticide Factsheets

    The Watershed Management Optimization Support Tool (WMOST) allows water-resource managers and planners to screen a wide range of practices for cost-effectiveness in achieving watershed or water utilities management goals.

  18. Healthy Watersheds Protection

    MedlinePlus

    ... habitat loss from warmer water temperatures associated with climate change already has been observed in the southern ... altered water flow and availability, invasive species and climate change. Healthy Watersheds EPA Awards Healthy Watersheds Consortium ...

  19. Island Watershed Activity.

    ERIC Educational Resources Information Center

    Benson, Rod

    2003-01-01

    Describes a 90-minute "Island Watershed" activity to help earth science students understand the concept of the water cycle. Introduces a surface waters unit appropriate for students in grades 7-10. Includes watershed project guidelines. (Author/KHR)

  20. Bull Trout Population and Habitat Surveys in the Middle Fork Willamette and McKenzie Rivers, Annual Report 2002.

    SciTech Connect

    Seals, Jason; Reis, Kelly

    2003-10-01

    Bull trout in the Willamette River Basin were historically distributed throughout major tributaries including the Middle Fork Willamette and McKenzie rivers. Habitat degradation, over-harvest, passage barriers, fish removal by rotenone, and hybridization and competition with non-native brook trout are all likely factors that have led to the decline of bull trout in the Willamette Basin (Ratliff and Howell 1992). The U.S. Fish and Wildlife Service listed the Columbia River bull trout population segment as Threatened under the federal Endangered Species Act in 1998. Four bull trout populations were isolated in the upper Willamette River following the construction of flood control dams on the South Fork McKenzie River, McKenzie River, and Middle Fork Willamette River that created Cougar, Trail Bridge, and Hills Creek reservoirs. Buchanan et al. (1997) described the population in the main stem McKenzie as 'of special concern', the South Fork McKenzie population as 'high risk of extinction', the population above Trail Bridge Reservoir as 'high risk of extinction', and bull trout in the Middle Fork Willamette as 'probably extinct'. Various management efforts such as strict angling regulations and passage improvement projects have been implemented to stabilize and rehabilitate bull trout habitat and populations in the McKenzie River over the past 10 years. Since 1997, bull trout fry from Anderson Creek on the upper McKenzie River have been transferred to the Middle Fork Willamette basin above Hills Creek Reservoir in an attempt to re-establish a reproducing bull trout population. This project was developed in response to concerns over the population status and management of bull trout in the McKenzie and Middle Fork Willamette Rivers by the Oregon Department of Fish and Wildlife during the early 1990s. The project was conducted under measure 9.3G(2) of the Columbia Basin Fish and Wildlife Program to monitor the status, life history, habitat needs, and limiting factors for

  1. Evaluation of Lower East Fork Poplar Creek Mercury Sources

    SciTech Connect

    Watson, David B.; Brooks, Scott C.; Mathews, Teresa J.; Bevelhimer, Mark S.; DeRolph, Chris; Brandt, Craig C.; Peterson, Mark J.; Ketelle, Richard

    2016-06-01

    This report summarizes a 3-year research project undertaken to better understand the nature and magnitude of mercury (Hg) fluxes in East Fork Poplar Creek (EFPC). This project addresses the requirements of Action Plan 1 in the 2011 Oak Ridge Reservation-wide Comprehensive Environmental Response, Compensation, and Liability Act Five Year Review (FYR). The Action Plan is designed to address a twofold 2011 FYR issue: (1) new information suggests mobilization of mercury from the upper and lower EFPC streambeds and stream banks is the primary source of mercury export during high-flow conditions, and (2) the current Record of Decision did not address the entire hydrologic system and creek bank or creek bed sediments. To obtain a more robust watershed-scale understanding of mercury sources and processes in lower EFPC (LEFPC), new field and laboratory studies were coupled with existing data from multiple US Department of Energy programs to develop a dynamic watershed and bioaccumulation model. LEFPC field studies for the project focused primarily on quantification of streambank erosion and an evaluation of mercury dynamics in shallow groundwater adjacent to LEFPC and potential connection to the surface water. The approach to the stream bank study was innovative in using imagery from kayak floats’ surveys from the headwaters to the mouth of EFPC to estimate erosion, coupled with detailed bank soil mercury analyses. The goal of new field assessments and modeling was to generate a more holistic and quantitative understanding of the watershed and the sources, flux, concentration, transformation, and bioaccumulation of inorganic mercury (IHg) and methylmercury (MeHg). Model development used a hybrid approach that dynamically linked a spreadsheet-based physical and chemical watershed model to a systems dynamics, mercury bioaccumulation model for key fish species. The watershed model tracks total Hg and MeHg fluxes and concentrations by examining upstream inputs, floodplain

  2. Age, growth, and mortality of introduced flathead catfish in Atlantic rivers and a review of other populations

    USGS Publications Warehouse

    Kwak, T.J.; Pine, William E.; Waters, D.S.

    2006-01-01

    Knowledge of individual growth and mortality rates of an introduced fish population is required to determine the success and degree of establishment as well as to predict the fish's impact on native fauna. The age and growth of flathead catfish Pylodictis olivaris have been studied extensively in the species' native and introduced ranges, and estimates have varied widely. We quantified individual growth rates and age structure of three introduced flathead catfish populations in North Carolina's Atlantic slope rivers using sagittal otoliths, determined trends in growth rates over time, compared these estimates among rivers in native and introduced ranges, and determined total mortality rates for each population. Growth was significantly faster in the Northeast Cape Fear River (NECFR) than in the Lumber and Neuse rivers. Fish in the NECFR grew to a total length of 700 mm by age 7, whereas fish in the Neuse and Lumber river populations reached this length by 8 and 10 years, respectively. The growth rates of fish in all three rivers were consistently higher than those of native riverine populations, similar to those of native reservoir populations, and slower than those of other introduced riverine populations. In general, recent cohorts (1998-2001 year-classes) in these three rivers exhibited slower growth among all ages than did cohorts previous to the 1998 year-class. The annual total mortality rate was similar among the three rivers, ranging from 0.16 to 0.20. These mortality estimates are considerably lower than those from the Missouri and Mississippi rivers, suggesting relatively low fishing mortality for these introduced populations. Overall, flathead catfish populations in reservoirs grow faster than those in rivers, the growth rates of introduced populations exceed those of native populations, and eastern United States populations grow faster than those in western states. Such trends constitute critical information for understanding and managing local

  3. Emerald Lake Watershed study: Introduction and site description

    SciTech Connect

    Tonnessen, K.A. )

    1991-07-01

    The Emerald Lake Watershed study was organized to investigate the effects of acidic deposition on high-elevation watersheds and surface waters of the Sierra Nevada, California. Some of the results of this comprehensive study of aquatic and terrestrial ecosystems at a small, headwater basin are presented in four papers in this series. The watershed study site is in Sequoia National Park, on the western slope of the Sierra Nevada. This glacial cirque is located in the upper Marble Fork of the Kaweah River. This 120-ha watershed ranges from Alta Peak (3,416 m) down to Emerald Lake (2,400 m). Most of the watershed surface area is exposed granite and granodiorite rocks, with limited coverage (about 20%) by thin, acidic soils. The hydrology of the basin is dominated by snowmelt runoff during March-June. Emerald Lake, a glacial tarn, is 2.72 ha in area, with a maximum depth of 10.5 m. Surface waters are poorly buffered and dominated by calcium and bicarbonate. Most of the yearly precipitation falls as dilute snow (pH5.2-5.4), with acidic rain storms sampled during May-October.

  4. Geochemical mass-balance in a small forested watershed in southwestern Pennsylvania

    USGS Publications Warehouse

    Witt, Emitt C.; Bikerman, Michael

    1991-01-01

    An intensive hydrologic investigation of the North Fork Bens Creek Watershed on Laurel Hill in southwestern Pennsylvania was made during 1984-85. Precipitation was sampled weekly, and stream water was sampled monthly and during selected storms for discharge and chemical composition. The watershed is underlain by sandstone and sandy shale consisting of quartz, feldspar, muscovite, chlorite, calcite, and kaolinite. Watershed chemical flux for the sum of Ca++, Mg++, Na+, and K+ shows that solutes from wet deposition account for 19 to 21 percent of the load in runoff from the watershed. Cation exchange and weathering account for the net changes in the chemistry of streamflow. Alteration of orthoclase, muscovite, chlorite, and albite to kaolinite accounts for 36 percent of the neutralization of H+ resulting from precipitation input and carbonic-acid weathering. Dissolution of calcite accounts for 34 percent of H+ neutralization. Dissolution of aluminum-bearing minerals in the soil matrix accounts for 25 percent of H+ neutralization.

  5. Anadronous Fish Habitat Enhancement for the Middle Fork and Upper Salmon River, 1988 Annual Report.

    SciTech Connect

    Andrews, John

    1990-01-01

    The wild and natural salmon and steelhead populations in the Middle Fork and Upper Salmon River are at a critical low. Habitat enhancement through decreasing sediment loads, increasing vegetative cover, removing passage barriers, and providing habitat diversity is imperative to the survival of these specially adapted fish, until passage problems over the Columbia River dams are solved. Personnel from the Boise and Sawtooth National Forests completed all construction work planned for 1988. In Bear Valley, 1573 feet of juniper revetment was constructed at eleven sites, cattle were excluded from 1291 feet of streambanks to prevent bank breakdown, and a small ephemeral gully was filled with juniper trees. Work in the Upper Salmon Drainage consisted of constructing nine rock sills/weirs, two rock deflectors, placing riprap along forty feet of streambank, construction of 2.1 miles of fence on private lands, and opening up the original Valley Creek channel to provide spring chinook passage to the upper watershed. A detailed stream survey of anadromous fish habitat covering 72.0 miles of streams in the Middle Fork Sub-basin was completed.

  6. Bedload measurements, East Fork River, Wyoming

    PubMed Central

    Leopold, Luna B.; Emmett, William W.

    1976-01-01

    A bedload trap in the riverbed provided direct quantitative measurement of debris-transport rate in the East Fork River, Wyoming, a basin of 466 km2 drainage area. Traction load moves only during the spring snow melt season. Data collected in three spring runoff seasons during which a peak flow of 45 m3/s occurred showed that transport rate is correlated with power expenditure of the flowing water and at high flows becomes directly proportional to power as suggested by Bagnold. PMID:16592302

  7. 14. Roaring Fork Motor Nature Trail, Place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  8. 33 CFR 117.315 - New River, South Fork.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false New River, South Fork. 117.315 Section 117.315 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.315 New River, South Fork. (a) The...

  9. South Fork Telephone Switchboard Building, oblique view of (W) and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, oblique view of (W) and (S) sides, view to northeast - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  10. South Fork Telephone Switchboard Building, interior west room showing hardwood ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, interior west room showing hardwood floor; view south - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  11. South Fork Telephone Switchboard Building, oblique view of east side; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, oblique view of east side; view northwest - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  12. South Fork Latrine, interior showing head with steel tank mounted ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, interior showing head with steel tank mounted to wall; view south - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  13. South Fork Telephone Switchboard Building, general view in setting showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, general view in setting showing (N) side; view (S) - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  14. Protect and Restore Red River Watershed, 2007-2008 Annual Report.

    SciTech Connect

    Bransford, Stephanie

    2009-05-04

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. The Nez Perce Tribe (NPT) and the Nez Perce National Forest (NPNF) have formed a partnership in completing watershed restoration activities, and through this partnership more work is accomplished by sharing funding and resources in our effort. The Nez Perce Tribe began watershed restoration projects within the Red River Watershed of the South Fork Clearwater River in 2001. Progress has been made in restoring the watershed through road decommissioning and culvert replacement. From completing a watershed assessment to two NEPA efforts and a final stream restoration design, we will begin the effort of restoring the mainstem channel of Red River to provide spawning and rearing habitat for anadromous and resident fish species. Roads have been surveyed and prioritized for removal or improvement as well as culverts being prioritized for replacement to accommodate fish passage throughout the watershed. Another major, and extremely, important component of this project is the Red River Meadow Conservation Easement. We have begun the process of pursuing a conservation easement on approximately 270 acres of prime meadow habitat (Red River runs through this meadow and is prime spawning and rearing habitat).

  15. Restore McComas Meadows; Meadow Creek Watershed, 2005-2006 Annual Report.

    SciTech Connect

    McRoberts, Heidi

    2006-07-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. Watershed restoration projects within the Meadow Creek watershed are coordinated and cost shared with the Nez Perce National Forest. The Nez Perce Tribe began watershed restoration projects within the Meadow Creek watershed of the South Fork Clearwater River in 1996. Progress has been made in restoring the watershed by excluding cattle from critical riparian areas through fencing, planting trees in riparian areas within the meadow and its tributaries, prioritizing culverts for replacement to accommodate fish passage, and decommissioning roads to reduce sediment input. During this contract period work was completed on two culvert replacement projects; Doe Creek and a tributary to Meadow Creek. Additionally construction was also completed for the ditch restoration project within McComas Meadows. Monitoring for project effectiveness and trends in watershed conditions was also completed. Road decommissioning monitoring, as well as stream temperature, sediment, and discharge were completed.

  16. Restore McComas Meadows; Meadow Creek Watershed, 2004-2005 Annual Report.

    SciTech Connect

    McRoberts, Heidi

    2005-12-01

    The Nez Perce Tribe Department of Fisheries Resource Management, Watershed Division approaches watershed restoration with a ridge-top to ridge-top approach. Watershed restoration projects within the Meadow Creek watershed are coordinated and cost shared with the Nez Perce National Forest. The Nez Perce Tribe began watershed restoration projects within the Meadow Creek watershed of the South Fork Clearwater River in 1996. Progress has been made in restoring the watershed by excluding cattle from critical riparian areas through fencing, planting trees in riparian areas within the meadow and its tributaries, prioritizing culverts for replacement to accommodate fish passage, and decommissioning roads to reduce sediment input. During this contract period, bids were solicited and awarded for two culvert replacement projects on Doe Creek, and a tributary to Meadow Creek. Additionally, NEPA and permits were completed for the ditch restoration project within McComas Meadows. Due to delays in cultural resource surveys, the contract was not awarded for the performance of the ditch restoration. It will occur in 2005. Monitoring for project effectiveness and trends in watershed conditions was also completed. Road decommissioning monitoring, as well as stream temperature, sediment, and discharge were completed.

  17. Regulation of Replication Fork Advance and Stability by Nucleosome Assembly

    PubMed Central

    Prado, Felix; Maya, Douglas

    2017-01-01

    The advance of replication forks to duplicate chromosomes in dividing cells requires the disassembly of nucleosomes ahead of the fork and the rapid assembly of parental and de novo histones at the newly synthesized strands behind the fork. Replication-coupled chromatin assembly provides a unique opportunity to regulate fork advance and stability. Through post-translational histone modifications and tightly regulated physical and genetic interactions between chromatin assembly factors and replisome components, chromatin assembly: (1) controls the rate of DNA synthesis and adjusts it to histone availability; (2) provides a mechanism to protect the integrity of the advancing fork; and (3) regulates the mechanisms of DNA damage tolerance in response to replication-blocking lesions. Uncoupling DNA synthesis from nucleosome assembly has deleterious effects on genome integrity and cell cycle progression and is linked to genetic diseases, cancer, and aging. PMID:28125036

  18. DNA Copy Number Control Through Inhibition of Replication Fork Progression

    PubMed Central

    Nordman, Jared T.; Kozhevnikova, Elena N.; Verrijzer, C. Peter; Pindyurin, Alexey V.; Andreyeva, Evgeniya N.; Shloma, Victor V.; Zhimulev, Igor F.; Orr-Weaver, Terry L.

    2014-01-01

    Summary Proper control of DNA replication is essential to ensure faithful transmission of genetic material and to prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell cycle regulation. Importantly, DNA replication is highly influenced by developmental cues. In Drosophila, specific regions of the genome are repressed for DNA replication during differentiation by the SNF2 domain-containing protein SUUR through an unknown mechanism. We demonstrate that SUUR is recruited to active replication forks and mediates repression of DNA replication by directly inhibiting replication fork progression instead of functioning as a replication fork barrier. Mass-spec identification of SUUR associated proteins identified the replicative helicase member CDC45 as a SUUR-associated protein, supporting a role for SUUR directly at replication forks. Our results reveal that control of eukaryotic DNA copy number can occur through inhibition of replication fork progression. PMID:25437540

  19. Regulation of Replication Fork Advance and Stability by Nucleosome Assembly.

    PubMed

    Prado, Felix; Maya, Douglas

    2017-01-24

    The advance of replication forks to duplicate chromosomes in dividing cells requires the disassembly of nucleosomes ahead of the fork and the rapid assembly of parental and de novo histones at the newly synthesized strands behind the fork. Replication-coupled chromatin assembly provides a unique opportunity to regulate fork advance and stability. Through post-translational histone modifications and tightly regulated physical and genetic interactions between chromatin assembly factors and replisome components, chromatin assembly: (1) controls the rate of DNA synthesis and adjusts it to histone availability; (2) provides a mechanism to protect the integrity of the advancing fork; and (3) regulates the mechanisms of DNA damage tolerance in response to replication-blocking lesions. Uncoupling DNA synthesis from nucleosome assembly has deleterious effects on genome integrity and cell cycle progression and is linked to genetic diseases, cancer, and aging.

  20. A Watershed Cooperative Addresses Short and Long-Term Perspectives for the Management of Harmful Algae at a Southwestern Ohio Drinking Water Reservoir

    EPA Science Inventory

    The multi-agency East Fork Watershed Cooperative (EFWCoop) has focused discussion and consequent leveraged monitoring efforts to understand how to ensure water safety in the short term. The EFWCoop is also collecting the dense data sets required to consider potential options for...

  1. Depth and substrate as determinants of distribution of juvenile flathead sole (Hippoglossoides elassodon) and rock sole (Pleuronectes bilineatus), in Kachemak Bay, Alaska

    USGS Publications Warehouse

    Abookire, Alisa A.; Norcross, Brenda L.

    1998-01-01

    Three transects in Kachemak Bay, Alaska, were sampled in September 1994, May and August 1995, and February, May, and August 1996. Juvenile flathead sole, Hippoglossoides elassodon, and rock sole, Pleuronectes bilineatus, were the most abundant flatfishes, comprising 65-85% of all fiatfishes captured at any period. Collections of fish and sediments were made at regular depth contour intervals of l0 m. Habitat distribution was described by depth at 10 m increments and sediment percent weights of gravel, sand, and mud. Year-round habitat of flathead sole age-0 was primarily from 40 to 60 m, and age-1 habitat was primarily from 40 to 80 m. Summer habitat of rock sole age-0 and -1 was from 10 to 30 m, and in winter they moved offshore to depths of up to 150 m. Both age classes of flathead sole were most abundant on mixed mud sediments, while age-1 were also in high abundance on muddy sand sediments. Rock sole age-0 and -1 were most abundant on sand, though age-1 were also found on a variety of sediments both finer and coarser grained than sand. Flathead sole and rock sole had distinctive depth and sediment habitats. When habitat overlap occurred between the species, it was most often due to rock sole moving offshore in the winter. Abundances were not significantly different among seasons for age-1 flatfishes.

  2. Evaluation of a single application of Neonicotnoid and multi-application contact insecticides for flatheaded borer management in field grown Acer rubrum L. cultivars

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two trials evaluated insecticides for flatheaded borer (Chrysobothris femorata [Olivier]) control and red maple (Acer rubrum L.) cultivar growth over a 4-year period. Soil-applied systemic insecticides (acephate, imidacloprid, clothianidin, dinotefuran, and thiamethoxam) and trunk-applied contact i...

  3. Impacts of Water Levels on Breeding Canada Geese and the Methodology for Mitigation and Enhancement in the Flathead Drainage, 1983 Annual Report.

    SciTech Connect

    Ball, I. Joseph

    1984-01-01

    The lower Flathead River Canada goose study was initiated to determine goose population trends and the effects of water level fluctuations on Canada goose nest and brood habitat, as a result of releases from Kerr Dam. This report presents data collected during the 1983 field season (15 February to 30 September, 1983) as part of an ongoing project. (DT)

  4. Seasonal movement and habitat use by sub-adult bull trout in the upper Flathead River system, Montana

    USGS Publications Warehouse

    Muhlfeld, Clint C.; Marotz, Brian

    2005-01-01

    Despite the importance of large-scale habitat connectivity to the threatened bull trout Salvelinus confluentus, little is known about the life history characteristics and processes influencing natural dispersal of migratory populations. We used radiotelemetry to investigate the seasonal movements and habitat use by subadult bull trout (i.e., fish that emigrated from natal streams to the river system) tracked for varying durations from 1999 to 2002 in the upper Flathead River system in northwestern Montana. Telemetry data revealed migratory (N = 32 fish) and nonmigratory (N = 35 fish) behavior, indicating variable movement patterns in the subadult phase of bull trout life history. Most migrating subadults (84%) made rapid or incremental downriver movements (mean distance, 33 km; range, 6–129 km) to lower portions of the river system and to Flathead Lake during high spring flows and as temperatures declined in the fall and winter. Bull trout subadults used complex daytime habitat throughout the upper river system, including deep runs that contained unembedded boulder and cobble substrates, pools with large woody debris, and deep lake-influenced areas of the lower river system. Our results elucidate the importance of maintaining natural connections and a diversity of complex habitats over a large spatial scale to conserve the full expression of life history traits and processes influencing the natural dispersal of bull trout populations. Managers should seek to restore and enhance critical river corridor habitat and remove migration barriers, where possible, for recovery and management programs.

  5. Flathead Lake Angler Survey; Monitoring Activities for the Hungry Horse Fisheries Mitigation Plan, 1992-1993 Final Report.

    SciTech Connect

    Evarts, Les; Hansen, Barry; DosSantos, Joe

    1994-02-01

    A roving creel survey was conducted on Flathead Lake in northwestern Montana from May 17, 1992 to May 19, 1993. The primary objective of the survey was to quantify the baseline fishery and exploitation rates existing prior to Hungry Horse Dam mitigation efforts. Anglers were counted on 308 occasions, comprising 5,618 fishing boats, 515 shore anglers, and 2,191 ice anglers. The party interviews represented 4,410 anglers, made up of 2,613 boat anglers, 787 shore anglers, and 1,010 ice anglers. A total of 47,883 angler days (190,108 angler hours) of pressure and a harvest of 42,979 fish (including lake trout, lake whitefish, yellow perch, bull trout, and westslope cutthroat trout) were estimated. Pressure was distributed between shore, boat, and ice anglers as 4%, 87%, and 9%, respectively. Seventynine percent of the total effort was directed at lake trout during the study period. Limited comparisons were made to previous creel surveys on Flathead Lake due to differences in methods and radical changes in the fishery. Potential sources of bias are explained in detail. Future creel surveys must employ methods consistent with this survey to obtain estimates that are statistically distinguishable.

  6. Lake trout (Salvelinus namaycush) suppression for bull trout (Salvelinus confluentus) recovery in Flathead Lake, Montana, North America

    USGS Publications Warehouse

    Hansen, Michael J.; Hansen, Barry S; Beauchamp, David A.

    2016-01-01

    Non-native lake trout Salvelinus namaycush displaced native bull trout Salvelinus confluentus in Flathead Lake, Montana, USA, after 1984, when Mysis diluviana became abundant following its introduction in upstream lakes in 1968–1976. We developed a simulation model to determine the fishing mortality rate on lake trout that would enable bull trout recovery. Model simulations indicated that suppression of adult lake trout by 75% from current abundance would reduce predation on bull trout by 90%. Current removals of lake trout through incentivized fishing contests has not been sufficient to suppress lake trout abundance estimated by mark-recapture or indexed by stratified-random gill netting. In contrast, size structure, body condition, mortality, and maturity are changing consistent with a density-dependent reduction in lake trout abundance. Population modeling indicated total fishing effort would need to increase 3-fold to reduce adult lake trout population density by 75%. We conclude that increased fishing effort would suppress lake trout population density and predation on juvenile bull trout, and thereby enable higher abundance of adult bull trout in Flathead Lake and its tributaries.

  7. Ground water-surface water relations in the Flathead River valley near the proposed Cabin Creek coal mine, British Columbia, Canada

    USGS Publications Warehouse

    Moreland, J.A.; Liebscher, Hugh; Van Voast, W. A.; Feltis, R.D.

    1987-01-01

    The area of the proposed Cabin Creek coal mine was studied to obtain information needed to respond to questions posed by the International Joint Commission advisers concerning water resources near the international border. Specific interest focused on determining the extent and character of surficial material in the Flathead River valley, identifying gaining and losing reaches of the river and major tributaries, and documenting ambient water quality at selected sites. Thickness of the alluvial deposits depends on depth to underlaying Quaternary glacial deposits or Tertiary bedrock. The alluvial deposits in the Flathead River valley thin to a veneer of cobbles near the mouth of Couldrey Creek. Measurements of streamflow at 20 sites in the Flathead River valley indicate that water discharges from the alluvial deposits to most of the tributaries and to the river near the proposed mine. The Flathead River gains 0.87 cu m/sec (31 cu ft/sec) of flow near Howell Creek. The Flathead River and Couldrey Creek gained about 0.81 cu m/sec (28.5 cu ft/sec) of flow near the mouth of Couldrey Creek where bedrock crops out in the streambeds. Bedrock outcrops effectively interrupt the alluvial aquifer system between the proposed mine site and the international border. The Flathead River lost 0.87 cu m/sec (31 cu ft/sec) of flow between the bedrock outcrops and the international border; this streamflow loss enters alluvial deposits and flows across the international border as subsurface flow. Analysis of samples from 18 stream sites and 1 spring site indicates general trends in water quality. In Howell Creek, concentrations of calcium, magnesium, and sulfates increased slightly downstream. Conversely, samples from Sage and Couldrey Creeks indicate downstream increases in concentrations of calcium, magnesium, and alkalinity, but decreases in concentrations of sulfate. Water quality of Cabin Creek was relatively stable through the sampled reach. Decreased concentrations of calcium and

  8. Application of Watershed Ecological Risk Assessment Methods to Watershed Management

    EPA Science Inventory

    Watersheds are frequently used to study and manage environmental resources because hydrologic boundaries define the flow of contaminants and other stressors. Ecological assessments of watersheds are complex because watersheds typically overlap multiple jurisdictional boundaries,...

  9. Watersheds: where we live

    USGS Publications Warehouse

    Vandas, Stephen; Farrar, Frank

    1996-01-01

    We all live in a watershed. Animals and plants all live there with us. Everyone affects what happens in a watershed by how we treat the natural resources. So what is a watershed? It is the land area that drains water to a stream, river, lake, or ocean. Water travels over the Earth's surface across forest land, farm fields, pastures, suburban lawns, and city streets, or it seeps into the soil and makes its way to a stream as local ground water. Watersheds come in many different shapes and sizes. Some contain mountains and hills, and others are nearly flat. A watershed can be affected by many different activities and events. Construction of cities and towns, farming, logging, and the application and disposal of many garden and household chemicals can affect the quantity and quality of water flowing from a watershed.

  10. The Fork+ burnup measurement system: Design and first measurement campaign

    SciTech Connect

    Olson, C.E.; Bronowski, D.R.; McMurtry, W.; Ewing, R.; Jordan, R.; Rivard, D.

    1998-12-31

    Previous work with the original Fork detector showed that burnup as determined by reactor records could be accurately allocated to spent nuclear fuel assemblies. The original Fork detector, designed by Los Alamos National Laboratory, used an ion chamber to measure gross gamma count and a fission chamber to measure neutrons from an activation source, {sup 244}Cm. In its review of the draft Topical Report on Burnup Credit, the US Nuclear Regulatory Commission indicated it felt uncomfortable with a measurement system that depended on reactor records for calibration. The Fork+ system was developed at Sandia National Laboratories under the sponsorship of the Electric Power Research Institute with the aim of providing this independent measurement capability. The initial Fork+ prototype was used in a measurement campaign at the Maine Yankee reactor. The campaign confirmed the applicability of the sensor approach in the Fork+ system and the efficiency of the hand-portable Fork+ prototype in making fuel assembly measurements. It also indicated potential design modifications that will be necessary before the Fork+ can be used effectively on high-burnup spent fuel.

  11. New interpretation of Clarks Fork field, northern Bighorn basin, Montana

    SciTech Connect

    Johnson, J.S.; Lindsley-Griffin, N.

    1986-08-01

    Clarks Fork field is located at the northern edge of the Bighorn basin (T9S, R22E) in Carbon County, Montana. Production was first established in 1944 by General Petroleum Corporation in the Cretaceous Peay Sandstone (basal Frontier) and was later extended to the Cretaceous Greybull (1949) and Lakota (1956) sandstones by British American. Total cumulative hydrocarbons from this field are 1,1789,193 bbl of oil and 3,061,522 mcf of gas, with Lakota sandstones being most productive. Lakota production occurs from a structural-stratigraphic trap in an east-west-trending channel on the axis of Clarks Fork anticline, geographically near the center of the township. Our structural reinterpretation of Clarks Fork field suggests that Elk Basin anticline is a northwest extension of the Elk Basin field anticline. The Elk Basin thrust truncates the north limb of the fold and does not strike to the northwest, as shown by earlier interpretations. They interpret a northwest-striking thrust in the center of the township as a splay off the Elk Basin thrust, and have named it the Clarks Fork thrust. The Clarks Fork anticline is located on the hanging wall of Clarks Fork thrust. Subsurface maps indicate the Clarks Fork area has not been fully developed. Stratigraphic traps in the Lakota and Greybull sandstones are present in several areas of the township. Structural traps in the center and northwest portions of the township may also exist.

  12. Effect of the Operation of Kerr and Hungry Horse Dams on the Reproduction Success of Kokanee in the Flathead River System, 1986 Annual Progress Report.

    SciTech Connect

    Beattie, Will; Clancey, Patrick

    1987-03-01

    The 1985 kokanee spawning run in the Flathead system was the strongest in five years. Escapement to the Flathead River system was 147,000 fish, including 123,000 in McDonald Creek and an estimated 20,000 in the main stem. Enumeration of spawners and redds in the Flathead River was hindered by high fall flows and early freezing in November. The upstream spawning migration from Flathead Lake began in late August. Schools of kokanee were seen six miles above the lake on September 4. We counted 1,156 redds in Flathead Lake, distributed primarily along the southeastern shore. An unusually high proportion (90 percent) of lakeshore spawning occurred in the zone above minimum pool, where egg mortality is very high because of exposure from drawdown. Escapement to the Swan River was 1,350 fish. Four year old (III+) fish comprised 95 percent of the spawning run in the Flathead system. This continues a five-year trend toward dominance of the III+ year class. The age composition of spawners has varied considerably for the past 15 years. The average size of spawning fish was 365 mm, which is identical to the average size of the parent year class in 1981. One of the goals of managing Flathead kokanee is to produce mature fish 300-330 mm in length. In the main stem Flathead River, pre-emergent survival was 80 percent. Survival in McDonald Creek, unaffected by hydroelectric operations, was 83 percent. Sampling showed few hatched alevins, probably due to unusually cold winter temperatures. Egg survival at Blue Bay, a spawning area on Flathead Lake where redds are concentrated below minimum pool, varied in relation to depth and dissolved oxygen concentration in the substrate. Eggs survived 78 days at 2,880 feet where dissolved oxygen was 5.7 mg/l. Eggs survived 35 days at 2,870 feet where dissolved oxygen concentration averaged 2.9 mg/l. Low dissolved oxygen contributed to poor survival to emergence at all elevations in Blue Ray. Experiments in Skidoo Bay confirmed that survival of

  13. Fork gratings based on ferroelectric liquid crystals.

    PubMed

    Ma, Y; Wei, B Y; Shi, L Y; Srivastava, A K; Chigrinov, V G; Kwok, H-S; Hu, W; Lu, Y Q

    2016-03-21

    In this article, we disclose a fork grating (FG) based on the photo-aligned ferroelectric liquid crystal (FLC). The Digital Micro-mirror Device based system is used as a dynamic photomask to generated different holograms. Because of controlled anchoring energy, the photo alignment process offers optimal conditions for the multi-domain FLC alignment. Two different electro-optical modes namely DIFF/TRANS and DIFF/OFF switchable modes have been proposed where the diffraction can be switched either to no diffraction or to a completely black state, respectively. The FLC FG shows high diffraction efficiency and fast response time of 50µs that is relatively faster than existing technologies. Thus, the FLC FG may pave a good foundation toward optical vertices generation and manipulation that could find applications in a variety of devices.

  14. Chemical and biological sensing using tuning forks

    DOEpatents

    Tao, Nongjian; Boussaad, Salah

    2012-07-10

    A device for sensing a chemical analyte is disclosed. The device is comprised of a vibrating structure having first and second surfaces and having an associated resonant frequency and a wire coupled between the first and second surfaces of the vibrating structure, wherein the analyte interacts with the wire and causes a change in the resonant frequency of the vibrating structure. The vibrating structure can include a tuning fork. The vibrating structure can be comprised of quartz. The wire can be comprised of polymer. A plurality of vibrating structures are arranged in an array to increase confidence by promoting a redundancy of measurement or to detect a plurality of chemical analytes. A method of making a device for sensing a chemical analyte is also disclosed.

  15. Determining Absolute Zero Using a Tuning Fork

    NASA Astrophysics Data System (ADS)

    Goldader, Jeffrey D.

    2008-04-01

    The Celsius and Kelvin temperature scales, we tell our students, are related. We explain that a change in temperature of 1°C corresponds to a change of 1 Kelvin and that atoms and molecules have zero kinetic energy at zero Kelvin, -273°C. In this paper, we will show how students can derive the relationship between the Celsius and Kelvin temperature scales using a simple, well-known physics experiment. By making multiple measurements of the speed of sound at different temperatures, using the classic physics experiment of determining the speed of sound with a tuning fork and variable-length tube, they can determine the temperature at which the speed of sound is zero—absolute zero.

  16. Wireless tuning fork gyroscope for biomedical applications

    NASA Astrophysics Data System (ADS)

    Abraham, Jose K.; Varadan, Vijay K.; Whitchurch, Ashwin K.; Sarukesi, K.

    2003-07-01

    This paper presents the development of a Bluetooth enabled wireless tuning fork gyroscope for the biomedical applications, including gait phase detection system, human motion analysis and physical therapy. This gyroscope is capable of measuring rotation rates between -90 and 90 and it can read the rotation information using a computer. Currently, the information from a gyroscope can trigger automobile airbag deployment during rollover, improve the accuracy and reliability of GPS navigation systems and stabilize moving platforms such as automobiles, airplanes, robots, antennas, and industrial equipment. Adding wireless capability to the existing gyroscope could help to expand its applications in many areas particularly in biomedical applications, where a continuous patient monitoring is quite difficult. This wireless system provides information on several aspects of activities of patients for real-time monitoring in hospitals.

  17. Spatial and seasonal dynamics of brook trout populations inhabiting a central Appalachian watershed

    USGS Publications Warehouse

    Petty, J.T.; Lamothe, P.J.; Mazik, P.M.

    2005-01-01

    We quantified the watershed-scale spatial population dynamics of brook trout Salvelinus fontinalis in the Second Fork, a third-order tributary of Shavers Fork in eastern West Virginia. We used visual surveys, electrofishing, and mark-recapture techniques to quantify brook trout spawning intensity, population density, size structure, and demographic rates (apparent survival and immigration) throughout the watershed. Our analyses produced the following results. Spawning by brook trout was concentrated in streams with small basin areas (i.e., segments draining less than 3 km2), relatively high alkalinity (>10 mg CaCO3/L), and high amounts of instream cover. The spatial distribution of juvenile and small-adult brook trout within the watershed was relatively stable and was significantly correlated with spawning intensity. However, no such relationship was observed for large adults, which exhibited highly variable distribution patterns related to seasonally important habitat features, including instream cover, stream depth and width, and riparian canopy cover. Brook trout survival and immigration rates varied seasonally, spatially, and among size-classes. Differential survival and immigration tended to concentrate juveniles and small adults in small, alkaline streams, whereas dispersal tended to redistribute large adults at the watershed scale. Our results suggest that spatial and temporal variations in spawning, survival, and movement interact to determine the distribution, abundance, and size structure of brook trout populations at a watershed scale. These results underscore the importance of small tributaries for the persistence of brook trout in this watershed and the need to consider watershed-scale processes when designing management plans for Appalachian brook trout populations. ?? Copyright by the American Fisheries Society 2005.

  18. Hydrologic data for the Obed River watershed, Tennessee

    USGS Publications Warehouse

    Knight, Rodney R.; Wolfe, William J.; Law, George S.

    2014-01-01

    The Obed River watershed drains a 520-square-mile area of the Cumberland Plateau physiographic region in the Tennessee River basin. The watershed is underlain by conglomerate, sandstone, and shale of Pennsylvanian age, which overlie Mississippian-age limestone. The larger creeks and rivers of the Obed River system have eroded gorges through the conglomerate and sandstone into the deeper shale. The largest gorges are up to 400 feet deep and are protected by the Wild and Scenic Rivers Act as part of the Obed Wild and Scenic River, which is managed by the National Park Service. The growing communities of Crossville and Crab Orchard, Tennessee, are located upstream of the gorge areas of the Obed River watershed. The cities used about 5.8 million gallons of water per day for drinking water in 2010 from Lake Holiday and Stone Lake in the Obed River watershed and Meadow Park Lake in the Caney Fork River watershed. The city of Crossville operates a wastewater treatment plant that releases an annual average of about 2.2 million gallons per day of treated effluent to the Obed River, representing as much as 10 to 40 percent of the monthly average streamflow of the Obed River near Lancing about 35 miles downstream, during summer and fall. During the past 50 years (1960–2010), several dozen tributary impoundments and more than 2,000 small farm ponds have been constructed in the Obed River watershed. Synoptic streamflow measurements indicate a tendency towards dampened high flows and slightly increased low flows as the percentage of basin area controlled by impoundments increases.

  19. Developing a Watershed Challenge

    ERIC Educational Resources Information Center

    Roman, Harry T.

    2010-01-01

    This article presents a watershed challenge that gives students an opportunity to investigate the challenge of using a watershed area as a site for development, examining the many aspects of this multifaceted problem. This design challenge could work well in a team-based format, with students taking on specific aspects of the challenges and…

  20. Master Watershed Stewards.

    ERIC Educational Resources Information Center

    Comer, Gary L.

    The Master Watershed Stewards (MWS) Program is a pilot project (developed through the cooperation of the Ohio State University Extension Logan and Hardin County Offices and the Indian Lake Watershed Project) offering the opportunity for communities to get involved at the local level to protect their water quality. The program grew out of the…

  1. Little Known Facts about the Common Tuning Fork.

    ERIC Educational Resources Information Center

    Ong, P. P.

    2002-01-01

    Explains the physical principles of the tuning fork which has a common use in teaching laboratories. Includes information on its vibration, frequency of vibration, elasticity, and reasons for having two prongs. (YDS)

  2. 17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK THAT MIXED COFFEE BEANS AS THEY WERE HUSKED - Hacienda Cafetalera Santa Clara, Coffee Mill, KM 19, PR Route 372, Hacienda La Juanita, Yauco Municipio, PR

  3. 9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

  4. 8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

  5. TRAIP regulates replication fork recovery and progression via PCNA

    PubMed Central

    Feng, Wanjuan; Guo, Yingying; Huang, Jun; Deng, Yiqun; Zang, Jianye; Huen, Michael Shing-Yan

    2016-01-01

    PCNA is a central scaffold that coordinately assembles replication and repair machineries at DNA replication forks for faithful genome duplication. Here, we describe TRAIP (RNF206) as a novel PCNA-interacting factor that has important roles during mammalian replicative stress responses. We show that TRAIP encodes a nucleolar protein that migrates to stalled replication forks, and that this is accomplished by its targeting of PCNA via an evolutionarily conserved PIP box on its C terminus. Accordingly, inactivation of TRAIP or its interaction with the PCNA clamp compromised replication fork recovery and progression, and leads to chromosome instability. Together, our findings establish TRAIP as a component of the mammalian replicative stress response network, and implicate the TRAIP-PCNA axis in recovery of stalled replication forks. PMID:27462463

  6. 9. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH OF LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING W. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  7. 8. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH OF LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING N. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  8. 117. Laurel Fork Viaduct. Elevation view of this 545 1939 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    117. Laurel Fork Viaduct. Elevation view of this 545 1939 steel girder viaduct. Example of structure with plain reinforced concrete arches. Looking northwest. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  9. Assembly of Slx4 signaling complexes behind DNA replication forks.

    PubMed

    Balint, Attila; Kim, TaeHyung; Gallo, David; Cussiol, Jose Renato; Bastos de Oliveira, Francisco M; Yimit, Askar; Ou, Jiongwen; Nakato, Ryuichiro; Gurevich, Alexey; Shirahige, Katsuhiko; Smolka, Marcus B; Zhang, Zhaolei; Brown, Grant W

    2015-08-13

    Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress.

  10. Environmental Assessment Deicer Recovery at Grand Forks AFB, North Dakota

    DTIC Science & Technology

    2004-12-15

    completed application to this office for further review. We also request the opportunity for complete review of applications for renewal or...NO 58205~6434 September 30, 2004 ND SHPO Ref.: 97 .. Q527av, Draft FONSI, Deicer Recovery Operation, Grand Forks AFB, NO. Dear Ms. Strom: We have...reviewed the Finding ofNo Significant Impact for a deicer recovery operation (draft version) at the Grand Forks Air Force Base, NO. We have no

  11. Environmental Assessment Tent City at Grand Forks AFB, North Dakota

    DTIC Science & Technology

    2004-11-15

    thunderstorms. Winters are long and severe with almost continuous snow cover. The spring and fall seasons are generally short transition periods. The...from the northwest during the late fall, winter, and spring , and from the southeast during the summer. Grand Forks County is included in the ND Air...drainage system. At Manvel, ND, approximately 10 miles northeast of Grand Forks AFB, the mean discharge of the Turtle River is 50.3 feet cubed per

  12. Force regulated dynamics of RPA on a DNA fork

    PubMed Central

    Kemmerich, Felix E.; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

    2016-01-01

    Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg2+ concentrations, such that human RPA can melt DNA in absence of force. PMID:27016742

  13. Pingo scars in the Mission Valley, northwest Montana -- Implications for paleoclimate and the Flathead lobe of the Cordilleran ice sheet

    SciTech Connect

    Levish, D.R.; Klinger, R.E.; Ostenaa, D.A. )

    1993-04-01

    More than 2,000 closed depressions on the floor of the Mission Valley have previously been interpreted as kettles from a late Wisconsin advance of the Flathead lobe of the Cordilleran ice sheet. However, these depressions are encircled by low ridges or ramparts, a distinguishing characteristic of collapsed pingos and pingo scars in areas of active and former permafrost. The morphology of these ramparts, their internal structure, and the complex crosscutting patterns of depressions and ramparts, demonstrate that these closed depressions in the Mission Valley are pingo scars. The pingo scars commonly occur in clusters. Often annular ramparts are truncated by other ramparts and smaller pingo scars are superimposed on larger ones. The scars range in diameter from 20 to 400 m; have circular, oval, and composite shapes; and have ramparts that range in height from 1 to 10 m. South of Ronan, Montana, the density of pingo scars is as high as 50--60/km[sup 2], and pingo scars are associated with relic frost mounds and thermokarst features. A 30-m-long trench excavated across the rampart of a pingo scar exposed laminated silt and clay of glacial Lake Missoula. Upturned beds, recumbent folds, and minor thrust faults record the outward displacement of material during pingo growth. Normal faults cut these compressional features and indicate extension during later pingo collapse. The pingo scars formed in glacial Lake Missoula sediment of the Mission Valley constrain the southern extent of the late Wisconsin advance of the Flathead lobe and document the existence of permafrost following the most recent draining of glacial lake Missoula.

  14. Home range and space use patterns of flathead catfish during the summer-fall period in two Missouri streams

    USGS Publications Warehouse

    Vokoun, Jason C.; Rabeni, Charles F.

    2005-01-01

    Flathead catfish Pylodictis olivaris were radio-tracked in the Grand River and Cuivre River, Missouri, from late July until they moved to overwintering habitats in late October. Fish moved within a definable area, and although occasional long-distance movements occurred, the fish typically returned to the previously occupied area. Seasonal home range was calculated with the use of kernel density estimation, which can be interpreted as a probabilistic utilization distribution that documents the internal structure of the estimate by delineating portions of the range that was used a specified percentage of the time. A traditional linear range also was reported. Most flathead catfish (89%) had one 50% kernel-estimated core area, whereas 11% of the fish split their time between two core areas. Core areas were typically in the middle of the 90% kernel-estimated home range (58%), although several had core areas in upstream (26%) and downstream (16%) portions of the home range. Home-range size did not differ based on river, sex, or size and was highly variable among individuals. The median 95% kernel estimate was 1,085 m (range, 70– 69,090 m) for all fish. The median 50% kernel-estimated core area was 135 m (10–2,260 m). The median linear range was 3,510 m (150–50,400 m). Fish pairs with core areas in the same and neighboring pools had static joint space use values of up to 49% (area of intersection index), indicating substantial overlap and use of the same area. However, all fish pairs had low dynamic joint space use values (<0.07; coefficient of association), indicating that fish pairs were temporally segregated, rarely occurring in the same location at the same time.

  15. Effect of the Operation of Kerr and Hungry Horse Dams on the Reproductive Success of Kokanee in the Flathead System, 1987 Final Report.

    SciTech Connect

    Beattie, Will; Zubik, Raymond; Clancey, Patrick

    1988-05-01

    Studies of kokanee reproductive success in the Flathead system from 1981 to 1987 have assessed the losses in fish production attributable to hydroelectric operations. We estimated that the Flathead Lake shoreline spawning stock has lost at least 50,000 fish annually, since Kerr Dam was completed in 1938. The Flathead River spawning stock has lost 95,000 spawners annually because of the operations of Hungry Horse Dam. Lakeshore spawning has been adversely affected because Flathead Lake has been drafted to minimum pool during the winter when kokanee eggs are incubating in shallow shoreline redds. Egg mortality from exposure and desiccation of kokanee redds has increased since the mid 1970's. When the lake was drafted more quickly and held longer at minimum pool. Escapement surveys in the early 1950's, and a creel survey in the early 1960's have provided a baseline to which the present escapement levels can be compared, and loss estimated. Main stem Flathead River spawning has also declined since the mid 1970's when fluctuating discharge from Hungry Horse Dam during the spawning and incubation season exposed redds at the river margin and increased mortality. This decline followed an increase in main stem spawning in the late 1950's through the mid 1960's attributable to higher winter water temperature and relatively stable discharge from Hungry Horse Dam. Spawning escapement in the main stem exceeded 300,000 kokanee in the early 1970's as a result. Spawning in spring-influenced sites has comprised 35 percent of the main stem escapement from 1979 to 1986. We took that proportion of the early 1970's escapement (105,000) as the baseline against which to measure historic loss. Agricultural and suburban development has contributed less significantly to degradation of kokanee spawning habitat in the river system and on the Flathead Lake shoreline. Their influence on groundwater quality and substrate composition has limited reproductive success in few sites. Studies of the

  16. Impacts of Water Level Fluctuations on Kokanee Reproduction in Flathead Lake; Effects of Operation of Kerr and Hungry Horse Dam on Reproductive Success, 1983 Annual Report.

    SciTech Connect

    Decker-Hess, Janet; McMullin, Steve L.

    1983-11-01

    Koktneesalmon (Oncorhvnchusnerka), the land-locked form of sockeye salmon, were originally introduced to Flathead Lake in 1916. My 1933, kokanee had become established in the lake and provided a popular summer trolling fishery as well as a fall snagging fishery in shoreline areas. Presently, Flathead Lake supports the second highest fishing pressure of any lake or reservoir in Montana (Montana Department of Fish and Game 1976). During 1981-82, the lake provided 168,792 man-days of fishing pressure. Ninety-two percent of the estimated 536,870 fish caught in Flathead Lake in 1981-82 were kokanee salmon. Kokanee also provided forage for bull trout seasonally and year round for lake trout. Kokanee rear to maturity in Flathead Lake, then return to various total grounds to spawn. Spawning occurred in lake outlet streams, springs, larger rivers and lake shoreline areas in suitable but often limited habitat. Shoreline spawning in Flathead Lake was first documented in the mid-1930's. Spawning kokanee were seized from shoreline areas in 1933 and 21,000 cans were processed and packed for distribution to the needy. Stefanich (1953 and 1954) later documented extensive but an unquantified amount of spawning along the shoreline as well as runs in Whitefish River and McDonald Creek in the 1950's. A creel census conducted in 1962-63 determined 11 to 13 percent of the kokanee caught annually were taken during the spawning period (Robbins 1966). During a 1981-82 creel census, less than one percent of the fishermen on Flathead Lake were snagging kokanee (Graham and Fredenberg 1982). The operation of Kerr Dam, located below Flathead Lake on the Flathead River, has altered seasonal fluctuations of Flathead Lake. Lake levels presently remain high during kokanee spawning in November and decline during the incubation and emergence periods. Groundwater plays an important role in embryo and fry survival in redds of shoreline areas exposed by lake drawdown. Stefanich (1954) and Domrose (1968

  17. Watersheds in disordered media

    NASA Astrophysics Data System (ADS)

    Andrade, Joséi, Jr.; Araújo, Nuno; Herrmann, Hans; Schrenk, Julian

    2015-02-01

    What is the best way to divide a rugged landscape? Since ancient times, watersheds separating adjacent water systems that flow, for example, toward different seas, have been used to delimit boundaries. Interestingly, serious and even tense border disputes between countries have relied on the subtle geometrical properties of these tortuous lines. For instance, slight and even anthropogenic modifications of landscapes can produce large changes in a watershed, and the effects can be highly nonlocal. Although the watershed concept arises naturally in geomorphology, where it plays a fundamental role in water management, landslide, and flood prevention, it also has important applications in seemingly unrelated fields such as image processing and medicine. Despite the far-reaching consequences of the scaling properties on watershed-related hydrological and political issues, it was only recently that a more profound and revealing connection has been disclosed between the concept of watershed and statistical physics of disordered systems. This review initially surveys the origin and definition of a watershed line in a geomorphological framework to subsequently introduce its basic geometrical and physical properties. Results on statistical properties of watersheds obtained from artificial model landscapes generated with long-range correlations are presented and shown to be in good qualitative and quantitative agreement with real landscapes.

  18. Spatial Patterns of Mercury Bioaccumulation in the Upper Clark Fork River Basin, MT

    NASA Astrophysics Data System (ADS)

    Staats, M. F.; Langner, H.; Moore, J. N.

    2010-12-01

    The Upper Clark Fork River Basin (UCFRB) in Montana has a legacy of historic gold/silver mine waste that contributes large quantities of mercury into the watershed. Mercury bioaccumulation at higher levels of the aquatic food chain, such as the mercury concentration in the blood of pre-fledge osprey, exhibit an irregular spatial signature based on the location of the nests throughout the river basin. Here we identify regions with a high concentration of bioavailable mercury and the major factors that allow the mercury to bioaccumulate within trophic levels. This identification is based on the abundance of mercury sources and the potential for mercury methylation. To address the source term, we did a survey of total mercury in fine sediments along selected UCFRB reaches, along with the assessment of environmental river conditions (percentage of backwaters/wetlands, water temperature and pH, etc). In addition, we analyzed the mercury levels of a representative number of macroinvertebrates and fish from key locations. The concentration of total mercury in sediment, which varies from reach to reach (tributaries of the Clark Fork River, <0.05 mg/kg to the main stem of the river, >5mg/kg) affects the concentration of mercury found at various trophic levels. However, reaches with a low supply of mine waste-derived mercury can also yield substantial concentrations of mercury in the biota, due to highly favorable conditions for mercury methylation. We identify that the major environmental factor that affects the methylation potential in the UCFRB is the proximity and connectivity of wetland areas to the river.

  19. Impacts of Water Levels on Breeding Canada Geese and Methods for Mitigation and Management in the Southern Flathead Valley, Montana, 1983-1987 Final Report.

    SciTech Connect

    Mackey, Dennis L.; Gregory, Shari K.; Matthews, William C. Jr.; Claar, James J.; Ball, I. Joseph

    1987-11-01

    Kerr Hydroelectric Dam is located at the south end of Flathead Lake, controls water levels on the lake and the Flathead River below the dam, and is currently operated as a load control facility. Current operation of Kerr Dam creates the greatest yearly water level fluctuations on both the lake and river during the Canada goose (Branta canadensis moffitti) brood and nesting period. Data collected from 1980-1982 indicated that goose nest numbers on the river were lower than during the 1950's, and that brood habitat on the lake may be limiting the goose population there. Our study was conducted from 1983-1987 to determine the effects of Kerr Dam operation on Canada goose populations and habitat on the south half of Flathead Lake and the Flathead River, and to formulate management and mitigation recommendations. Nesting geese on the river appeared to be negatively affected by a lack of nest sites free from predators, and responded to available artificial nest structures with an increase in nest numbers and nesting success. Under current dam operation, river channel depths and widths do not discourage access to nesting islands by mammalian predators during some years and high predation on ground nests occurs. Intensively used brood areas on the lake and river were identified and described. Brood habitat on the lake was lower in quality and quantity than on the river due to dam operations. Gosling mortality on the lake was high, almost 2 times higher than on the river. Lake broods expended more energy obtaining food than river broods. Losses of brood habitat in the form of wet meadow marshes were documented and mitigation options developed. Management/mitigation alternatives and monitoring methods for nesting and brooding geese were identified.

  20. Validation of in situ networks via field sampling: case study in the South Fork Experimental Watershed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The calibration and validation of soil moisture remote sensing products is complicated by the logistics of installing a soil moisture network for a long term period in an active landscape. Therefore, these stations are located along field boundaries or in non-representative sites with regards to so...

  1. Predicting Fecal Indicator Bacteria Concentrations in the South Fork Broad River Watershed Using Virtual Beach

    EPA Science Inventory

    Virtual Beach (VB) is a decision support tool that constructs site-specific statistical models to predict fecal indicator bacteria (FIB) at recreational beaches. Although primarily designed for making decisions regarding beach closures or issuance of swimming advisories based on...

  2. The south fork experimental watershed: Soil moisture and precipitation network for satellite validation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A recently deployed long term network for the study of soil moisture and precipitation was deployed in north central iowa, in cooperation between USDA and NASA. This site will be a joint calibration/validation network for the Soil Moisture Active Passive (SMAP) and Global Precipitation Measurement ...

  3. 77 FR 55796 - Sand Lick Fork Watershed Restoration Project; Daniel Boone National Forest, KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... of 0.6 miles of Powell County Road 212 to Forest Service maintenance, conversion of 3.1 miles of..., 212A, 2045, 2120 and the section of county road to be transferred to Forest Service maintenance... Forest Plan provides for the maintenance of the physical integrity of aquatic ecosystems,...

  4. Validation of in Situ Networks Via Field Sampling: Case Study in the South Fork Experimental Watershed

    NASA Astrophysics Data System (ADS)

    Cosh, M. H.; McKee, L.; Bindlish, R.; Coopersmith, E. J.; Jackson, T. J.; Prueger, J. H.

    2014-12-01

    The calibration and validation of soil moisture remote sensing products is complicated by the logistics of installing a soil moisture network for a long term period in an active landscape. Therefore, these stations are located along field boundaries or in non-representative sites with regards to soil type or soil moisture. The representative character of this network can only be established by large scale field sampling to provide a calibration dataset. A team of samplers were deployed twice a week for the summer of 2014 to collect surface soil moisture data across a variety of land covers at 44 sites. These samples were compared and scaled to the domain to get a better understanding of the large scale soil moisture distributions and dynamics. In addition, comparisons are made to the Soil Moisture Ocean Salinity (SMOS) soil moisture product for the length of the network installation.

  5. An Adaptive Watershed Management Assessment Based on Watershed Investigation Data

    NASA Astrophysics Data System (ADS)

    Kang, Min Goo; Park, Seung Woo

    2015-05-01

    The aim of this study was to assess the states of watersheds in South Korea and to formulate new measures to improve identified inadequacies. The study focused on the watersheds of the Han River basin and adopted an adaptive watershed management framework. Using data collected during watershed investigation projects, we analyzed the management context of the study basin and identified weaknesses in water use management, flood management, and environmental and ecosystems management in the watersheds. In addition, we conducted an interview survey to obtain experts' opinions on the possible management of watersheds in the future. The results of the assessment show that effective management of the Han River basin requires adaptive watershed management, which includes stakeholders' participation and social learning. Urbanization was the key variable in watershed management of the study basin. The results provide strong guidance for future watershed management and suggest that nonstructural measures are preferred to improve the states of the watersheds and that consistent implementation of the measures can lead to successful watershed management. The results also reveal that governance is essential for adaptive watershed management in the study basin. A special ordinance is necessary to establish governance and aid social learning. Based on the findings, a management process is proposed to support new watershed management practices. The results will be of use to policy makers and practitioners who can implement the measures recommended here in the early stages of adaptive watershed management in the Han River basin. The measures can also be applied to other river basins.

  6. An adaptive watershed management assessment based on watershed investigation data.

    PubMed

    Kang, Min Goo; Park, Seung Woo

    2015-05-01

    The aim of this study was to assess the states of watersheds in South Korea and to formulate new measures to improve identified inadequacies. The study focused on the watersheds of the Han River basin and adopted an adaptive watershed management framework. Using data collected during watershed investigation projects, we analyzed the management context of the study basin and identified weaknesses in water use management, flood management, and environmental and ecosystems management in the watersheds. In addition, we conducted an interview survey to obtain experts' opinions on the possible management of watersheds in the future. The results of the assessment show that effective management of the Han River basin requires adaptive watershed management, which includes stakeholders' participation and social learning. Urbanization was the key variable in watershed management of the study basin. The results provide strong guidance for future watershed management and suggest that nonstructural measures are preferred to improve the states of the watersheds and that consistent implementation of the measures can lead to successful watershed management. The results also reveal that governance is essential for adaptive watershed management in the study basin. A special ordinance is necessary to establish governance and aid social learning. Based on the findings, a management process is proposed to support new watershed management practices. The results will be of use to policy makers and practitioners who can implement the measures recommended here in the early stages of adaptive watershed management in the Han River basin. The measures can also be applied to other river basins.

  7. Hydrologic and hydraulic analyses for the Black Fork Mohican River Basin in and near Shelby, Ohio

    USGS Publications Warehouse

    Huitger, Carrie A.; Ostheimer, Chad J.; Koltun, G.F.

    2016-05-06

    Hydrologic and hydraulic analyses were done for selected reaches of five streams in and near Shelby, Richland County, Ohio. The U.S. Geological Survey (USGS), in cooperation with the Muskingum Watershed Conservancy District, conducted these analyses on the Black Fork Mohican River and four tributaries: Seltzer Park Creek, Seltzer Park Tributary, Tuby Run, and West Branch. Drainage areas of the four stream reaches studied range from 0.51 to 60.3 square miles. The analyses included estimation of the 10-, 2-, 1-, and 0.2-percent annual-exceedance probability (AEP) flood-peak discharges using the USGS Ohio StreamStats application. Peak discharge estimates, along with cross-sectional and hydraulic structure geometries, and estimates of channel roughness coefficients were used as input to step-backwater models. The step-backwater water models were used to determine water-surface elevation profiles of four flood-peak discharges and a regulatory floodway. This study involved the installation of, and data collection at, a streamflow-gaging station (Black Fork Mohican River at Shelby, Ohio, 03129197), precipitation gage (Rain gage at Reservoir Number Two at Shelby, Ohio, 405209082393200), and seven submersible pressure transducers on six selected river reaches. Two precipitation-runoff models, one for the winter events and one for nonwinter events for the headwaters of the Black Fork Mohican River, were developed and calibrated using the data collected. With the exception of the runoff curve numbers, all other parameters used in the two precipitation-runoff models were identical. The Nash-Sutcliffe model efficiency coefficients were 0.737, 0.899, and 0.544 for the nonwinter events and 0.850 and 0.671 for the winter events. Both of the precipitation-runoff models underestimated the total volume of water, with residual runoff ranging from -0.27 inches to -1.53 inches. The results of this study can be used to assess possible mitigation options and define flood hazard areas that

  8. Clean Watersheds Needs Survey

    EPA Pesticide Factsheets

    The Clean Watershed Needs Survey is a national assessment of the future capital cost for publicly owned wastewater collection and treatment facilities to meet the Clean Water Act's water quality goals.

  9. Merrimack River Watershed Communities

    EPA Pesticide Factsheets

    Listing of all communities included in each of the hydrologic unit code (HUC) 8, 10, and 12 boundaries for the Merrimack River Watershed and city locations for the EPA water quality monitoring stations.

  10. Mystic River Watershed

    EPA Pesticide Factsheets

    Information on the efforts of the US EPA, the Commonwealth of Massachusetts, the municipalities within the Mystic River Watershed and nongovernmental organizations to improve the water quality of the Mystic River.

  11. Watershed Restoration Project

    SciTech Connect

    Julie Thompson; Betsy Macfarlan

    2007-09-27

    In 2003, the U.S. Department of Energy issued the Eastern Nevada Landscape Coalition (ENLC) funding to implement ecological restoration in Gleason Creek and Smith Valley Watersheds. This project was made possible by congressionally directed funding that was provided through the US Department of Energy, Energy Efficiency and Renewable Energy, Office of the Biomass Program. The Ely District Bureau of Land Management (Ely BLM) manages these watersheds and considers them priority areas within the Ely BLM district. These three entities collaborated to address the issues and concerns of Gleason Creek and Smith Valley and prepared a restoration plan to improve the watersheds’ ecological health and resiliency. The restoration process began with watershed-scale vegetation assessments and state and transition models to focus on restoration sites. Design and implementation of restoration treatments ensued and were completed in January 2007. This report describes the restoration process ENLC undertook from planning to implementation of two watersheds in semi-arid Eastern Nevada.

  12. Healthy Watersheds Protection

    MedlinePlus

    ... habitat loss from warmer water temperatures associated with climate change already has been observed in the southern Appalachians ( ... altered water flow and availability, invasive species, and climate change. Top of Page How is a Healthy Watershed ...

  13. Effect of the Operation of Kerr and Hungry Horse Dams on the Reproductive Success of Kokanee in the Flathead System; Technical Addendum to the Final Report.

    SciTech Connect

    Beattie, Will; Tohtz, Joel

    1990-03-01

    This addendum to the Final Report presents results of research on the zooplankton and fish communities of Flathead Lade. The intent of the Study has been to identify the impacts of hydroelectric operations at Kerr and Hungry Horse Dam on the reproductive success of kokanee an to propose mitigation for these impacts. Recent changes in the trophic ecology of the lake, have reduced the survival of kokanee. In the last three year the Study has been redirected to identify, if possible, the biological mechanisms which now limit kokanee survival, and to test methods of enhancing the kokanee fishery by artificial supplementation. These studies were necessary to the formulation of mitigation plans. The possibility of successfully rehabilitating the kokanee population, is the doubt because of change in the trophic ecology of the system. This report first presents the results of studies of the population dynamics of crustacean zooplankton, upon which planktivorous fish depend. A modest effort was directed to measuring the spawning escapement of kokanee in 1988. Because of its relevance to the study, we also report assessments of 1989 kokanee spawning escapement. Hydroacoustic assessment of the abundance of all fish species in Flathead Lake was conducted in November, 1988. Summary of the continued efforts to document the growth rates and food habits of kokanee and lake whitefish are included in this report. Revised kokanee spawning and harvest estimates, and management implications of the altered ecology of Flathead Lake comprise the final sections of this addendum. 83 refs., 20 figs., 25 tabs.

  14. National Water-Quality Assessment Program; summary of pesticide data collected on East Fork Double Bayou, near Anahuac, Texas, March to September 1994

    USGS Publications Warehouse

    ,

    1995-01-01

    The Trinity River Basin study-unit assessment began in October 1991, with 2 years dedicated to planning, analyzing existing information, and designing data-collection networks, surveys, and studies. Then, a 3-year intensive data-collection program was initiated. The assessment followed guidelines provided by the National Water-Quality Assessment (NAWQA) Program National Synthesis team and considered suggestions made by the study unit's liaison committee. One of the issues selected for study concerned the quality of runoff in the coastal prairie. The study includes collecting streamflow, water-quality and watershed data on three streams, each representing watersheds in different parts of the coastal prairie. This fact sheet presents a summary of the pesticide data collected on East Fork Double Bayou from March to September 1994.

  15. Single strand transposition at the host replication fork

    PubMed Central

    Lavatine, Laure; He, Susu; Caumont-Sarcos, Anne; Guynet, Catherine; Marty, Brigitte; Chandler, Mick; Ton-Hoang, Bao

    2016-01-01

    Members of the IS200/IS605 insertion sequence family differ fundamentally from classical IS essentially by their specific single-strand (ss) transposition mechanism, orchestrated by the Y1 transposase, TnpA, a small HuH enzyme which recognizes and processes ss DNA substrates. Transposition occurs by the ‘peel and paste’ pathway composed of two steps: precise excision of the top strand as a circular ss DNA intermediate; and subsequent integration into a specific ssDNA target. Transposition of family members was experimentally shown or suggested by in silico high-throughput analysis to be intimately coupled to the lagging strand template of the replication fork. In this study, we investigated factors involved in replication fork targeting and analysed DNA-binding properties of the transposase which can assist localization of ss DNA substrates on the replication fork. We showed that TnpA interacts with the β sliding clamp, DnaN and recognizes DNA which mimics replication fork structures. We also showed that dsDNA can facilitate TnpA targeting ssDNA substrates. We analysed the effect of Ssb and RecA proteins on TnpA activity in vitro and showed that while RecA does not show a notable effect, Ssb inhibits integration. Finally we discuss the way(s) in which integration may be directed into ssDNA at the replication fork. PMID:27466393

  16. Chk1 promotes replication fork progression by controlling replication initiation.

    PubMed

    Petermann, Eva; Woodcock, Mick; Helleday, Thomas

    2010-09-14

    DNA replication starts at initiation sites termed replication origins. Metazoan cells contain many more potential origins than are activated (fired) during each S phase. Origin activation is controlled by the ATR checkpoint kinase and its downstream effector kinase Chk1, which suppresses origin firing in response to replication blocks and during normal S phase by inhibiting the cyclin-dependent kinase Cdk2. In addition to increased origin activation, cells deficient in Chk1 activity display reduced rates of replication fork progression. Here we investigate the causal relationship between increased origin firing and reduced replication fork progression. We use the Cdk inhibitor roscovitine or RNAi depletion of Cdc7 to inhibit origin firing in Chk1-inhibited or RNAi-depleted cells. We report that Cdk inhibition and depletion of Cdc7 can alleviate the slow replication fork speeds in Chk1-deficient cells. Our data suggest that increased replication initiation leads to slow replication fork progression and that Chk1 promotes replication fork progression during normal S phase by controlling replication origin activity.

  17. Replication fork dynamics and the DNA damage response.

    PubMed

    Jones, Rebecca M; Petermann, Eva

    2012-04-01

    Prevention and repair of DNA damage is essential for maintenance of genomic stability and cell survival. DNA replication during S-phase can be a source of DNA damage if endogenous or exogenous stresses impair the progression of replication forks. It has become increasingly clear that DNA-damage-response pathways do not only respond to the presence of damaged DNA, but also modulate DNA replication dynamics to prevent DNA damage formation during S-phase. Such observations may help explain the developmental defects or cancer predisposition caused by mutations in DNA-damage-response genes. The present review focuses on molecular mechanisms by which DNA-damage-response pathways control and promote replication dynamics in vertebrate cells. In particular, DNA damage pathways contribute to proper replication by regulating replication initiation, stabilizing transiently stalled forks, promoting replication restart and facilitating fork movement on difficult-to-replicate templates. If replication fork progression fails to be rescued, this may lead to DNA damage and genomic instability via nuclease processing of aberrant fork structures or incomplete sister chromatid separation during mitosis.

  18. 77 FR 47058 - Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-07

    ... Energy Regulatory Commission Middle Fork American River Hydroelectric Project Placer County Water Agency... comments on the draft environmental impact statement for the Middle Fork American River Project No. 2079... project. This meeting is posted on the Commission's calendar located at...

  19. Earthworm survival and behavior results from a Clark Fork River Superfund site: Grant-Kohrs Ranch N.H.S., Montana

    SciTech Connect

    Rader, B.R.; Nimmo, D.R.; Chapman, P.L.

    1995-12-31

    Concentrations of heavy metals in sediments and soils deposited along the floodplain of the Clark Fork River, within the boundaries of the Grant-Kohrs Ranch National Historic Site, have exceeded those typically found in uncontaminated soils. Upstream mining activities along the Clark Fork River in the Deer Lodge Valley, Montana, have produced substantial quantities of mine waste which have been deposited throughout the watershed. Releases and re-releases of these contaminated substances continue to occur, and appear to be preventing the germination and establishment of critical riparian plant species and depressing soil microbe activity. Slickens, bare spots devoid of all vegetation, occur frequently in the floodplain along the Clark Fork River. This research investigates the toxicity of slicken soils using a series of earthworm (Eisenia foetida andrei) survival and behavior tests. In dilution tests, earthworm survival was reduced significantly in as little as 12.5% slicken soil. Results from earthworm behavior tests currently being conducted using non-lethal slicken soil dilutions will also be presented.

  20. Confederated Tribes of the Umatilla Indian Reservation North Fork John Day River Basin Anadromous Fish Enhancement Project, Annual Report for FY 2000.

    SciTech Connect

    Macy, Tom L.; James, Gary A.

    2003-03-01

    The CTUIR North Fork John Day River Basin Anadromous Enhancement Project (NFJDAFEP) identified and prioritized stream reaches in The North Fork John day River basin for habitat improvements during the 2000 project period. Public out reach was emphasized during this first year of the project. We presented multiple funding and enhancement options to landowners. We concentrated on natural recovery methods, riparian fencing and off-stream livestock water developments. Under this BPA contract four riparian easements were signed protecting almost 5 miles of tributary streams. There are nine offstream water developments associated with these easements. Some landowners chose to participate in other programs based on Tribal outreach efforts. Two landowners chose NRCS programs for enhancement and one chose OWEB as a funding source. Two landowners implemented there own enhancement measures protecting 3 miles of stream. Cooperation between the NRCS/FSA/SWCDs and the Tribe to create joint projects and develop alternative funding scenarios for riparian enhancement was a major effort. The Tribe also worked with the North Fork John Day Watershed Council, USFS and ODFW to coordinate projects and support similar projects throughout the John Day Basin. We provided input to the John Day Summary prepared for the NWPPC by ODFW. The Tribe worked with the Umatilla National Forest on the Clear Creek Dredgetailings Rehabilitation project and coordinated regularly with USFS Fisheries, Hydrology and Range staff.

  1. Confederated Tribes of the Umatilla Indian Reservation North Fork John Day River Basin Anadromous Fish Enhancement Project, Annual Report for FY 2001.

    SciTech Connect

    Macy, Tom L.; James, Gary A.

    2003-03-01

    The CTUIR North Fork John Day River Basin Anadromous Enhancement Project (NFJDAFEP) identified and prioritized stream reaches in The North Fork John day River basin for habitat improvements during the 2000 project period. Public outreach was emphasized during this first year of the project. During the past year we concentrated on satisfying landowner needs, providing cost share alternatives, providing joint projects and starting implementation. We presented multiple funding and enhancement options to landowners. We concentrated on natural recovery methods, riparian fencing and offstream livestock water developments. Under this BPA contract four riparian easements have been signed protecting almost 5 miles of tributary streams. There are nine offstream water developments associated with these easements. Some landowners chose to participate in other programs based on Tribal outreach efforts. Some landowners chose NRCS programs for enhancement and others chose OWEB as a funding source. The exact amount of stream protection due to other funding sources probably exceeds that by BPA, however most would not have entered any program without initial Tribal outreach. Cooperation between the NRCS/FSA/SWCDs and the Tribe to create joint projects and develop alternative funding scenarios for riparian enhancement was a major effort. The Tribe also worked with the North Fork John Day Watershed Council, USFS and ODFW to coordinate projects and support similar projects throughout the John Day Basin.

  2. Geology and ground water resources of Grand Forks County

    USGS Publications Warehouse

    Hansen, Dan E.; Kume, Jack; Kelly, T.E.; Paulson, Q.F.

    1970-01-01

    Grand Forks County in northeastern North Dakota is underlain by glacial drift, westward-dipping Paleozoic and Mesozoic sedimentary rocks and Precambrian igneous and metamorphic rocks. Glacial drift that covers the bedrock reaches a maximum thickness of 455 feet. It can be differentiated into 5 drift sheets, each of which in turn can be separated into till units, lake clay and silt units, and sand and gravel units. Relief on the bedrock surface is much greater than that on the present glacial topography. In western Grand Forks County, the bedrock rises 600 feet from east to west at the Pembina escarpment, whereas the surface elevations rise only 300 feet.

  3. Bank stability and channel width adjustment, East Fork River, Wyoming.

    USGS Publications Warehouse

    Andrews, E.D.

    1982-01-01

    Frequent surveys of eight cross sections located in self-formed reaches of the East Fork River, Wyoming, during the 1974 snowmelt flood showed a close relation between channel morphology and scour and fill. Those cross sections narrower than the mean reach width filled at discharges less than bankfull and scoured at discharges greater than bankfull. Those cross sections wider than the mean reach width scoured at discharges less than bankfull and filled at discharges greater than bankfull. Bank stability, and to some extent the adjustment of stream channel width, in the East Fork River study reach appears to be controlled by the processes of scour and fill. -from Author

  4. Replication Termination: Containing Fork Fusion-Mediated Pathologies in Escherichia coli

    PubMed Central

    Dimude, Juachi U.; Midgley-Smith, Sarah L.; Stein, Monja; Rudolph, Christian J.

    2016-01-01

    Duplication of bacterial chromosomes is initiated via the assembly of two replication forks at a single defined origin. Forks proceed bi-directionally until they fuse in a specialised termination area opposite the origin. This area is flanked by polar replication fork pause sites that allow forks to enter but not to leave. The precise function of this replication fork trap has remained enigmatic, as no obvious phenotypes have been associated with its inactivation. However, the fork trap becomes a serious problem to cells if the second fork is stalled at an impediment, as replication cannot be completed, suggesting that a significant evolutionary advantage for maintaining this chromosomal arrangement must exist. Recently, we demonstrated that head-on fusion of replication forks can trigger over-replication of the chromosome. This over-replication is normally prevented by a number of proteins including RecG helicase and 3’ exonucleases. However, even in the absence of these proteins it can be safely contained within the replication fork trap, highlighting that multiple systems might be involved in coordinating replication fork fusions. Here, we discuss whether considering the problems associated with head-on replication fork fusion events helps us to better understand the important role of the replication fork trap in cellular metabolism. PMID:27463728

  5. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for fork and frame assembly... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  6. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Requirements for fork and frame assembly... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  7. 77 FR 39675 - Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ...-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining AGENCY: Forest Service, USDA... North Fork Burnt River Mining Record of Decision will replace and supercede the 2004 North Fork Burnt River Mining Record of Decision only where necessary to address the inadequacies identified by the...

  8. Hydraulic geometry and sediment data for the South Fork Salmon River, Idaho, 1985-86

    USGS Publications Warehouse

    Williams, Rhea P.; O'Dell, Ivalou; Megahan, Walter F.

    1989-01-01

    Hydraulic geometry, suspended-sediment, and bedload samples were collected at three sites in the upper reach of the South Fork Salmon River drainage basin from April 1985 to June 1986. Sites selected were South Fork Salmon River near Krassel Ranger Station, Buckhorn Creek, and North Fork Lick Creek. Results of the data collection are presented in this report.

  9. Watershed Central: A New Gateway to Watershed Information

    EPA Science Inventory

    Many communities across the country struggle to find the right approaches, tools and data to in their watershed plans. EPA recently posted a new Web site called "Watershed Central, a “onestop" tool, to help watershed organizations and others find key resources to protect their ...

  10. Watershed Central: Dynamic Collaboration for Improving Watershed Management (Philadelphia)

    EPA Science Inventory

    The Watershed Central web and wiki pages will be presented and demonstrated real-time as part of the overview of Web 2.0 collaboration tools for watershed management. The presentation portion will discuss how EPA worked with watershed practitioners and within the Agency to deter...

  11. Entering the watershed

    SciTech Connect

    Doppelt, B.; Scurlock, M.; Frissell, C.; Karr, J.

    1993-01-01

    The ecological integrity of a river is a direct function of the health of its watershed. Riverine pollution, habitat degradation, and extinction of aquatic biodiversity are all issues that must be addressed at the ecosystem level. The product of a two-year project established by The Pacific Rivers Council to develop new federal riverine protection and restoration policy alternatives, this book recommends a comprehensive new approach to river protection: a nationwide, strategic community- and ecosystem-based watershed restoration initiative founded upon principles of watershed dynamics, ecosystem function, and conservation biology. The book describes in detail the existing level of damage of rivers and species. A new, intensified national emphasis on rivers is presented. The flaws and gaps in existing policy are analyzed. The scientific underpinnings and management strategies needed in new policy are outlined. Specific policy proposals are made.

  12. Mercury Contributions from Flint Creek and other Tributaries to the Upper Clark Fork River in Northwestern Montana

    NASA Astrophysics Data System (ADS)

    Langner, H.; Young, M.; Staats, M. F.

    2013-12-01

    Methylmercury contamination in biota is a major factor diminishing the environmental quality of the Upper Clark Fork River (CFR), e.g. by triggering human consumption limits of fish. The CFR is subject to one of the largest Superfund cleanup projects in the US, but remediation and restoration is currently focused exclusively on other mining-related contaminants (As, Cu, Zn, Pb, Cd), which may be counterproductive with respect to the bio-availability of mercury, for example by creation of wetlands along mercury-contaminated reaches of the river. The identification and elimination of Hg sources is an essential step toward reducing the methylmercury exposure in the biota of the CFR watershed because a strong correlation exists between total mercury levels in river sediment and methylmercury levels in aquatic life. We analyzed duplicate samples from the top sediment layer of the main stem and significant tributaries to the Clark Fork River along a 240 km reach between Butte, MT and downstream of the Missoula Valley. Mercury concentrations were 1.3 × 1.6 (mean × SD, n = 35) in the main stem. Concentrations in tributaries varied widely (0.02 to 85 mg/kg) and seemed only loosely related to the number of historic precious metal mines in the watershed. In the upper reach of the CFR, elevated Hg levels are likely caused by residual contaminated sediments in the flood plain. Levels tend to decrease downstream until Drummond, MT, where Flint Creek contributes a significant amount of mercury, causing Hg levels in the main stem CFR to increase from 0.7 to 4 mg/kg. Levels continue to decrease downstream. Flint Creek is the single largest contributor of Hg to the CFR. Detailed sampling of the main stem Flint Creek and tributaries (26 sites) showed extremely high levels in two tributaries (22 to 85 mg/kg) where historic milling operations were located. Elimination of these point sources may be accomplished comparatively economically and may significantly reduce mercury levels in

  13. Sources of alkalinity and acidity along an acid mine drainage remediated stream in SE Ohio: Hewett Fork

    NASA Astrophysics Data System (ADS)

    Schleich, K. L.; Lopez, D. A.; Bowman, J. R.; Kruse, N. A.; Mackey, A. L.; VanDervort, D.; Korenowsky, R.

    2013-12-01

    In the remediation of acid mine drainage impacted streams, it is important to locate and quantify the sources of acidity and alkalinity inputs. These parameters affect the long-term recovery of the stream habitat. Previous studies have focused on treating the remediation of AMD as point source pollution, targeting the main acid seep for remediation. However, in the interest of biological and chemical recovery, it is important to understand how sources of alkalinity and acidity, throughout the stream, affect water and sediment quality. The Hewett Fork watershed in Southeastern Ohio is impacted by AMD from the AS-14 mine complex in Carbondale, Ohio. In attempts to remediate the stream, the water is being treated with a continuous alkaline input from a calcium oxide doser. While the section of watershed furthest downstream from the doser is showing signs of recovery, the water chemistry and aquatic life near the doser are still impacted. The objective of this study is to examine and model the chemistry of the tributaries of Hewett Fork to see how they contribute to the alkalinity and acidity budgets of the main stem of the stream. By examining the inputs of tributaries into the main stem, this project aims to understand processes occurring during remediation throughout the entire stream. Discharge was measured during a dry period in October, 2012 and at a high flow in May, 2013. Field parameters such as pH, TDS, DO, alkalinity and acidity were also determined. Low flow data collected during fall sampling shows variable flow along the stream path, the stream gains water from ground water at some points while it loses water at others, potentially due to variable elevation of the water table. Flow data collected during spring sampling shows that Hewett Fork is a gaining stream during that period with inputs from groundwater contributing to increasing flow downstream. When using this data to calculate the net alkalinity load along the stream, there are areas with alkaline

  14. MANAGING URBAN WATERSHED PATHOGEN CONTAMINATION

    EPA Science Inventory

    This document is written as a resource for state and local watershed managers who have the responsibility of managing pathogen contamination in urban watersheds. In addition it can be an information source for members of the public interested in watershed mitigation efforts aime...

  15. Comparison between Measured and Calculated Sediment Transport Rates in North Fork Caspar Creek, California

    NASA Astrophysics Data System (ADS)

    Kim, T. W.; Yarnell, S. M.; Yager, E.; Leidman, S. Z.

    2015-12-01

    Caspar Creek is a gravel-bedded stream located in the Jackson Demonstration State Forest in the coast range of California. The Caspar Creek Experimental Watershed has been actively monitored and studied by the Pacific Southwest Research Station and California Department of Forestry and Fire Protection for over five decades. Although total annual sediment yield has been monitored through time, sediment transport during individual storm events is less certain. At a study site on North Fork Caspar Creek, cross-section averaged sediment flux was collected throughout two storm events in December 2014 and February 2015 to determine if two commonly used sediment transport equations—Meyer-Peter-Müller and Wilcock—approximated observed bedload transport. Cross-section averaged bedload samples were collected approximately every hour during each storm event using a Helley-Smith bedload sampler. Five-minute composite samples were collected at five equally spaced locations along a cross-section and then sieved to half-phi sizes to determine the grain size distribution. The measured sediment flux values varied widely throughout the storm hydrographs and were consistently less than two orders of magnitude in value in comparison to the calculated values. Armored bed conditions, changing hydraulic conditions during each storm and variable sediment supply may have contributed to the observed differences.

  16. Watershed geomorphological characteristics

    USGS Publications Warehouse

    Fitzpatrick, Faith

    2016-01-01

    This chapter describes commonly used geomorphological characteristics that are useful for analyzing watershed-scale hydrology and sediment dynamics. It includes calculations and measurements for stream network features and areal basin characteristics that cover a range of spatial and temporal scales and dimensions of watersheds. Construction and application of longitudinal profiles are described in terms of understanding the three-dimensional development of stream networks. A brief discussion of outstanding problems and directions for future work, particularly as they relate to water-resources management, is provided. Notations with preferred units are given.

  17. A new record of a flathead fish (Teleostei: Platycephalidae) from China based on morphological characters and DNA barcoding

    NASA Astrophysics Data System (ADS)

    Qin, Yan; Song, Na; Zou, Jianwei; Zhang, Zhaohui; Cheng, Guangping; Gao, Tianxiang; Zhang, Xiumei

    2013-05-01

    A new record of Platycephalus sp.1 (sensu Nakabo, 2002) was documented based on morphological characters and DNA barcoding. We collected 174 specimens of the genus Platycephalus from Chinese coastal waters of Dongying, Qingdao, Zhoushan, and Beihai. Samples were identified as Platycephalus sp.1 morphologically. The coloration, meristic traits, and morphometric measurements are consistent with previously published records. In brief, it is an orange-brown flathead fish with dark brown spots scattered on head and body, lateral line scales 83 to 99 with one or two spine-bearing anteriormost pored scale, no yellow blotch on the caudal fin. Cytochrome oxidase I subunit (COI) gene fragments were sequenced for phylogenetic analysis. The mean evolutionary distance within the species Platycephalus sp.1 was 0.1%. Net evolutionary distances between Platycephalus sp.1 and other species of Platycephalus ranged from 10.8% to 19.7%, which is much greater than the threshold for species delimitation. The COI sequence analysis strongly supports the validity of Platyceohalus sp.1 at genetic level.

  18. Field trapping of the flathead oak borer Coroebus undatus (Coleoptera: Buprestidae) with different traps and volatile lures.

    PubMed

    Fürstenau, Benjamin; Quero, Carmen; Riba, Josep Ma; Rosell, Gloria; Guerrero, Angel

    2015-02-01

    The flathead oak borer Coroebus undatus F. (Coleoptera: Buprestidae) is one of the primary pests of cork oak Quercus suber L. in the Mediterranean region causing great economic losses to the cork industry. Very little is known about its biology and behavior and, so far, no control measures have been established. We present the results of a pilot study aimed to develop an efficient trapping method for monitoring this harmful pest. In a 3-year field study, purple-colored prism traps baited with a mixture of green leaf volatiles (GLVs) from the host have been shown the most effective combination to catch C. undatus adults (solely females) compared to other trap and lure types tested. Wavelength and reflectance measurements revealed that purple traps exhibit reflectance peak values similar to those found in the abdominal and elytral cuticle of both sexes, suggesting the involvement of visual cues for mate location in this species. The data presented are the first to demonstrate captures of adults of the genus Coroebus by an attractant-based trapping method.

  19. Determination of organochlorine pesticide concentrations in flathead mullet (Mugil cephalus) caught from the western Black Sea coast of Turkey.

    PubMed

    Bozcaarmutlu, Azra; Turna, Sema; Sapmaz, Canan; Yenisoy-Karakaş, Serpil

    2014-12-01

    The objective of this study was to determine the levels of 14 organochlorine pesticides (OCPs) in flathead mullet (Mugil cephalus) caught from the western Black Sea coast of Turkey. The fish samples were caught from five different locations of the western Black Sea coast of Turkey in August 2009. Organochlorine pesticides were extracted from the liver tissues, and then the levels of OCPs were measured using gas chromatography with an electron capture detector. Organochlorine pesticides were detected in all locations. The levels of total OCPs in fish samples ranged between 0.224 and 1.103 μg g(-1) dry weight in the western Black Sea coast of Turkey. DDT, beta-HCH, and endosulfan I were the dominant OCPs in the fish samples. The levels of DDT in fish samples ranged between 0.081 and 0.186 μg g(-1) dry weight. The levels of total HCH in fish samples ranged between 0.007 and 0.376 μg g(-1) dry weight in the western Black Sea coast of Turkey. Although the usage of OCPs was banned in Turkey, the results of this study clearly indicated the presence of OCPs in the western Black Sea coast of Turkey and exposure of living organisms to these chemicals.

  20. Mercury and Selenium Concentrations in Biofilm, Macroinvertebrates, and Fish Collected in the Yankee Fork of the Salmon River, Idaho, USA, and Their Potential Effects on Fish Health

    USGS Publications Warehouse

    Rhea, Darren T.; Farag, Aida M.; Harper, David D.; McConnell, Elizabeth; Brumbaugh, William G.

    2013-01-01

    The Yankee Fork is a large tributary of the Salmon River located in central Idaho, USA, with an extensive history of placer and dredge-mining activities. Concentrations of selenium (Se) and mercury (Hg) in various aquatic trophic levels were measured in the Yankee Fork during 2001 and 2002. Various measurements of fish health were also performed. Sites included four on the mainstem of the Yankee Fork and two off-channel sites in partially reclaimed dredge pools used as rearing habitat for cultured salmonid eggs and fry. Hg concentrations in whole mountain whitefish and shorthead sculpin ranged from 0.28 to 0.56 μg/g dry weight (dw), concentrations that are generally less than those reported to have significant impacts on fish. Biofilm and invertebrates ranged from 0.05 to 0.43 μg Hg/g dw. Se concentrations measured in biota samples from the Yankee Fork were greater than many representative samples collected in the Snake and Columbia watersheds and often exceeded literature-based toxic thresholds. Biofilm and invertebrates ranged from 0.58 to 4.66 μg Se/g dw. Whole fish ranged from 3.92 to 7.10 μg Se/g dw, and gonads ranged from 6.91 to 31.84 μg Se/g dw. Whole-body Se concentrations exceeded reported toxicological thresholds at three of four sites and concentrations in liver samples were mostly greater than concentrations shown to have negative impacts on fish health. Histological examinations performed during this study noted liver abnormalities, especially in shorthead sculpin, a bottom-dwelling species.

  1. Mechanism of DNA Replication in Drosophila Chromosomes: Structure of Replication Forks and Evidence for Bidirectionality

    PubMed Central

    Kriegstein, Henry J.; Hogness, David S.

    1974-01-01

    The replicating chromosomal DNA in Drosophila melanogaster cleavage nuclei has been visualized in the electron microscope as a serial array of closely spaced replicated regions created by pairs of diverging replication forks. The fine structure of the forks is very similar to that observed for the replication forks of bidirectionally replicating bacteriophage DNAs. However, the mean length of the single-stranded gaps in Drosophila forks is less than 200 nucleotide residues, much shorter than the gaps in phage forks. This difference in gap length corresponds to the observed difference in the size of Okazaki fragments from Drosophila and phage. Images PMID:4204203

  2. 80. Laurel Fork Creek Bridge #2. Example of a concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    80. Laurel Fork Creek Bridge #2. Example of a concrete slab bridge with T beams. It was built in 1937 and the wing walls were faced with stone to blend with its surroundings. Looking northeast. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  3. Forks and combs and DNA: the synthesis of branched oligodeoxyribonucleotides.

    PubMed Central

    Horn, T; Urdea, M S

    1989-01-01

    Nucleoside phosphoramidite derivatives containing two protected primary hydroxyl functions have been incorporated into synthetic oligonucleotides as 'branching monomers'. With selective deprotection, multiple identical copies of an additional oligonucleotide can be incorporated to form fork- or comb-like structures for use as signal amplification materials in nucleic acid hybridization assays. Images PMID:2780317

  4. New histone supply regulates replication fork speed and PCNA unloading

    PubMed Central

    Mejlvang, Jakob; Feng, Yunpeng; Alabert, Constance; Neelsen, Kai J.; Jasencakova, Zuzana; Zhao, Xiaobei; Lees, Michael; Sandelin, Albin; Pasero, Philippe; Lopes, Massimo

    2014-01-01

    Correct duplication of DNA sequence and its organization into chromatin is central to genome function and stability. However, it remains unclear how cells coordinate DNA synthesis with provision of new histones for chromatin assembly to ensure chromosomal stability. In this paper, we show that replication fork speed is dependent on new histone supply and efficient nucleosome assembly. Inhibition of canonical histone biosynthesis impaired replication fork progression and reduced nucleosome occupancy on newly synthesized DNA. Replication forks initially remained stable without activation of conventional checkpoints, although prolonged histone deficiency generated DNA damage. PCNA accumulated on newly synthesized DNA in cells lacking new histones, possibly to maintain opportunity for CAF-1 recruitment and nucleosome assembly. Consistent with this, in vitro and in vivo analysis showed that PCNA unloading is delayed in the absence of nucleosome assembly. We propose that coupling of fork speed and PCNA unloading to nucleosome assembly provides a simple mechanism to adjust DNA replication and maintain chromatin integrity during transient histone shortage. PMID:24379417

  5. NEW APPROACHES: Speed of sound in tuning fork metal

    NASA Astrophysics Data System (ADS)

    Narayanan, V. Anantha; Narayanan, Radha

    1996-11-01

    A procedure to find the speed of sound in tuning fork metal is described. The formula needed is extracted from the literature and explained. Since the equipment needed for this project is readily available in most high school and introductory level college science laboratories, this exercise can be done without any additional cost.

  6. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Roanoke. 9.65 Section 9.65 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL AMERICAN VITICULTURAL AREAS Approved American Viticultural Areas §...

  7. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false North Fork of Roanoke. 9.65 Section 9.65 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS AMERICAN VITICULTURAL AREAS Approved American Viticultural Areas §...

  8. 134. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    134. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN FALLS COUNTY, SOUTH OF HANSEN, IDAHO; VIEW OF LOW LINE AND POWER GATES, WEST VIEW. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  9. 132. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    132. FORKS DIVERSION, HIGH LINE AND LOW LINE CANALS, TWIN FALLS COUNTY, SOUTH OF HANSEN, IDAHO; POWER GATES FOR HYDRO-ELECTRIC. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  10. Endonuclease EEPD1 Is a Gatekeeper for Repair of Stressed Replication Forks*

    PubMed Central

    Kim, Hyun-Suk; Nickoloff, Jac A.; Wu, Yuehan; Williamson, Elizabeth A.; Sidhu, Gurjit Singh; Reinert, Brian L.; Jaiswal, Aruna S.; Srinivasan, Gayathri; Patel, Bhavita; Kong, Kimi; Burma, Sandeep; Lee, Suk-Hee; Hromas, Robert A.

    2017-01-01

    Replication is not as continuous as once thought, with DNA damage frequently stalling replication forks. Aberrant repair of stressed replication forks can result in cell death or genome instability and resulting transformation to malignancy. Stressed replication forks are most commonly repaired via homologous recombination (HR), which begins with 5′ end resection, mediated by exonuclease complexes, one of which contains Exo1. However, Exo1 requires free 5′-DNA ends upon which to act, and these are not commonly present in non-reversed stalled replication forks. To generate a free 5′ end, stalled replication forks must therefore be cleaved. Although several candidate endonucleases have been implicated in cleavage of stalled replication forks to permit end resection, the identity of such an endonuclease remains elusive. Here we show that the 5′-endonuclease EEPD1 cleaves replication forks at the junction between the lagging parental strand and the unreplicated DNA parental double strands. This cleavage creates the structure that Exo1 requires for 5′ end resection and HR initiation. We observed that EEPD1 and Exo1 interact constitutively, and Exo1 repairs stalled replication forks poorly without EEPD1. Thus, EEPD1 performs a gatekeeper function for replication fork repair by mediating the fork cleavage that permits initiation of HR-mediated repair and restart of stressed forks. PMID:28049724

  11. Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability.

    PubMed

    Schalbetter, Stephanie A; Mansoubi, Sahar; Chambers, Anna L; Downs, Jessica A; Baxter, Jonathan

    2015-08-18

    Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA precatenation formed. Although fork rotation predominates at replication termination, in vitro studies have suggested that it also occurs frequently during elongation. However, the factors that influence fork rotation and how rotation and precatenation may influence other replication-associated processes are unknown. Here we analyze the causes and consequences of fork rotation in budding yeast. We find that fork rotation and precatenation preferentially occur in contexts that inhibit topoisomerase action ahead of the fork, including stable protein-DNA fragile sites and termination. However, generally, fork rotation and precatenation are actively inhibited by Timeless/Tof1 and Tipin/Csm3. In the absence of Tof1/Timeless, excessive fork rotation and precatenation cause extensive DNA damage following DNA replication. With Tof1, damage related to precatenation is focused on the fragile protein-DNA sites where fork rotation is induced. We conclude that although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin.

  12. Homologous recombination restarts blocked replication forks at the expense of genome rearrangements by template exchange.

    PubMed

    Lambert, Sarah; Mizuno, Ken'ichi; Blaisonneau, Joël; Martineau, Sylvain; Chanet, Roland; Fréon, Karine; Murray, Johanne M; Carr, Antony M; Baldacci, Giuseppe

    2010-08-13

    Template switching induced by stalled replication forks has recently been proposed to underlie complex genomic rearrangements. However, the resulting models are not supported by robust physical evidence. Here, we analyzed replication and recombination intermediates in a well-defined fission yeast system that blocks replication forks. We show that, in response to fork arrest, chromosomal rearrangements result from Rad52-dependent nascent strand template exchange occurring during fork restart. This template exchange occurs by both Rad51-dependent and -independent mechanisms. We demonstrate that Rqh1, the BLM homolog, limits Rad51-dependent template exchange without affecting fork restart. In contrast, we report that the Srs2 helicase promotes both fork restart and template exchange. Our data demonstrate that template exchange occurs during recombination-dependent fork restart at the expense of genome rearrangements.

  13. Termination of DNA replication forks: "Breaking up is hard to do".

    PubMed

    Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

    2015-01-01

    To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

  14. Oak Grove Fork Habitat Improvement Project, 1988 Annual Report.

    SciTech Connect

    Bettin, Scott

    1989-04-01

    The Lower Oak Grove Fork of the Clackamas River is a fifth-order tributary of the Clackamas River drainage supporting depressed runs of coho and chinook salmon, and summer and winter steelhead. Habitat condition rating for the Lower Oak Grove is good, but smelt production estimates are below the average for Clackamas River tributaries. Limiting factors in the 3.8 miles of the Lower Oak Grove supporting anadromous fish include an overall lack of quality spawning and rearing habitat. Beginning in 1986. measures to improve fish habitat in the Lower Oak Grove were developed in coordination with the Oregon Department of Fish and Wildlife (ODF&W) and Portland General Electric (PGE) fisheries biologists. Prior to 1986, no measures had been applied to the stream to mitigate for PGE's storage and regulation of flows in the Oak Grove Fork (Timothy Lake, Harriet Lake). Catchable rainbow trout are stocked by ODF&W two or three times a year during the trout fishing season in the lowermost portion of the Oak Grove Fork near two Forest Service campgrounds (Ripplebrook and Rainbow). The 1987 field season marked the third year of efforts to improve fish habitat of the Lower Oak Grove Fork and restore anadromous fish production. The efforts included the development of an implementation plan for habitat improvement activities in the Lower Oak Grove Fork. post-project monitoring. and maintenance of the 1986 improvement structures. No new structures were constructed or placed in 1987. Fiscal year 1988 brought a multitude of changes which delayed implementation of plans developed in 1987. The most prominent change was the withdrawal of the proposed Spotted Owl Habitat Area (SOHA) which overlapped the Oak Grove project implementation area. Another was the change in the Forest Service biologist responsible for implementation and design of this project.

  15. USEPA WATERSHED APPROACH

    EPA Science Inventory

    The U.S. Environmental Protection Agency's Office of Research and Development has developed a well defined research plan to evaluate pollutants within watersheds. This plan is defined by long term goals and annual performance measures. The first goal is to provide the approache...

  16. Retrofitting for watershed drainage

    SciTech Connect

    Bennett, D.B. ); Heaney, J.P. )

    1991-09-01

    Over the past 8 years, degradation in Florida's Indian River Lagoon has taken the form of fish kills, reduced viable recreational and commercial fisheries, and loss of seagrass beds. Stormwater drainage practices in the watershed have been identified as the primary culprit in the slow demise of the lagoon. Specific drainage problems include an increased volume of freshwater runoff to the estuarine receiving water and deposition of organic sediments, reduced water clarity because of increased discharge of suspended solids and tea colored' groundwater - a result of drainage-canal-induced land dewatering, and eutrophication caused by nutrient loadings. In addition, poor flushing in lagoon segments makes runoff impacts even more damaging to the ecosystem. Recently, the lagoon has received national, regional, state, and local attention over its degradation and citizens' action and multi-agency efforts to restore it. To mitigate damage to the Indian River lagoon, agencies are considering alternatives such as retrofitting to reduce pollutant loads and implementing a more comprehensive watershed approach to stormwater management instead of individual controls on new development currently widely practiced. A comprehensive, long-term watershed control approach avoids unnecessary construction expenses, encourages cost-effective tradeoffs based on specific objectives, facilities performance monitoring, and accounts for cumulative impacts of continued growth in the watershed.

  17. WATERSHED BASED SURVEY DESIGNS

    EPA Science Inventory

    The development of watershed-based design and assessment tools will help to serve the multiple goals for water quality monitoring required under the Clean Water Act, including assessment of regional condition to meet Section 305(b), identification of impaired water bodies or wate...

  18. Designing for Watershed Inquiry

    ERIC Educational Resources Information Center

    Bodzin, Alec; Shive, Louise

    2004-01-01

    In this article, we describe a collaborative design initiative with three secondary school teachers to promote the use of Web-based inquiry in the context of a watershed investigation. Design interviews that focus on instructional goals and pedagogical beliefs of classroom teachers were conducted. The interview protocol used a curricular framework…

  19. Impacts of Water Levels on Breeding Canada Geese and the Methodology for Mitigation and Enhancement in the Flathead Drainage, 1984 Annual Report.

    SciTech Connect

    Mackey, Dennis L.

    1985-01-01

    The lower Flathead System Canada Goose Study was initiated to determine population trends and the effects of water level fluctuations on nest and brood habitat on the southern half of Flathead Lake and the lower Flathead River as a result of the operations of Kerr Dam. This report presents data collected during the 1984 field season as part of an ongoing project. Geese used Pablo, Kicking Horse, Ninepipe Reservoirs heavily during late summer and fall. Use of the river by geese was high during the winter, when the reservoirs were frozen, and during the breeding period. Most breeding geese left the river after broods fledged. Thirteen percent of the artificial tree nest structures on the river were used by nesting geese. Goose nest initiation on the river peaked the last week in March through the first week in April, and hatching peaked the first week in May. Predation was the most significant cause of nest loss on the river, and nest loss by flooding was not observed. Avian predation was the single largest factor contributing to nest loss on the lake. Habitat use was studied in 4 brood areas on the river and 8 brood areas on the lake, and available habitat was assessed for 2 portions of both the lake and the river. Brood habitat use was significantly different from the available habitat in all areas studied. On the lower river, broods used wheat fields, gravel bars, and shrub habitats. On the upper river, coniferous forest and shrub habitats were preferred. On the West Bay of the lake, brood areas consisted primarily of lawns and tall herbaceous habitat, while on the South Bay, marshes dominated the brood areas studied. Water levels on the river and lake affect both accessibility of these areas to brooding geese, and the ecology of the habitats preferred by geese. 43 refs., 24 figs., 31 tabs.

  20. Effect of variable annual precipitation and nutrient input on nitrogen and phosphorus transport from two Midwestern agricultural watersheds.

    PubMed

    Kalkhoff, S J; Hubbard, L E; Tomer, M D; James, D E

    2016-07-15

    Precipitation patterns and nutrient inputs affect transport of nitrate (NO3-N) and phosphorus (TP) from Midwest watersheds. Nutrient concentrations and yields from two subsurface-drained watersheds, the Little Cobb River (LCR) in southern Minnesota and the South Fork Iowa River (SFIR) in northern Iowa, were evaluated during 1996-2007 to document relative differences in timings and amounts of nutrients transported. Both watersheds are located in the prairie pothole region, but the SFIR exhibits a longer growing season and more livestock production. The SFIR yielded significantly more NO3-N than the LCR watershed (31.2 versus 21.3kgNO3-Nha(-1)y(-1)). The SFIR watershed also yielded more TP than the LCR watershed (1.13 versus 0.51kgTPha(-1)yr(-1)), despite greater TP concentrations in the LCR. About 65% of NO3-N and 50% of TP loads were transported during April-June, and <20% of the annual loads were transported later in the growing season from July-September. Monthly NO3-N and TP loads peaked in April from the LCR but peaked in June from the SFIR; this difference was attributed to greater snowmelt runoff in the LCR. The annual NO3-N yield increased with increasing annual runoff at a similar rate in both watersheds, but the LCR watershed yielded less annual NO3-N than the SFIR for a similar annual runoff. These two watersheds are within 150 km of one another and have similar dominant agricultural systems, but differences in climate and cropping inputs affected amounts and timing of nutrient transport.

  1. Effect of variable annual precipitation and nutrient input on nitrogen and phosphorus transport from two Midwestern agricultural watersheds

    USGS Publications Warehouse

    Kalkhoff, Stephen J.; Hubbard, Laura E.; Tomer, Mark D.; James, D.E.

    2016-01-01

    Precipitation patterns and nutrient inputs affect transport of nitrate (NO3-N) and phosphorus (TP) from Midwest watersheds. Nutrient concentrations and yields from two subsurface-drained watersheds, the Little Cobb River (LCR) in southern Minnesota and the South Fork Iowa River (SFIR) in northern Iowa, were evaluated during 1996–2007 to document relative differences in timings and amounts of nutrients transported. Both watersheds are located in the prairie pothole region, but the SFIR exhibits a longer growing season and more livestock production. The SFIR yielded significantly more NO3-N than the LCR watershed (31.2 versus 21.3 kg NO3-N ha− 1 y− 1). The SFIR watershed also yielded more TP than the LCR watershed (1.13 versus 0.51 kg TP ha− 1 yr− 1), despite greater TP concentrations in the LCR. About 65% of NO3-N and 50% of TP loads were transported during April–June, and < 20% of the annual loads were transported later in the growing season from July–September. Monthly NO3-N and TP loads peaked in April from the LCR but peaked in June from the SFIR; this difference was attributed to greater snowmelt runoff in the LCR. The annual NO3-N yield increased with increasing annual runoff at a similar rate in both watersheds, but the LCR watershed yielded less annual NO3-N than the SFIR for a similar annual runoff. These two watersheds are within 150 km of one another and have similar dominant agricultural systems, but differences in climate and cropping inputs affected amounts and timing of nutrient transport.

  2. Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation.

    PubMed

    Bermejo, Rodrigo; Doksani, Ylli; Capra, Thelma; Katou, Yuki-Mori; Tanaka, Hirokazu; Shirahige, Katsuhiko; Foiani, Marco

    2007-08-01

    DNA topoisomerases solve topological problems during chromosome metabolism. We investigated where and when Top1 and Top2 are recruited on replicating chromosomes and how their inactivation affects fork integrity and DNA damage checkpoint activation. We show that, in the context of replicating chromatin, Top1 and Top2 act within a 600-base-pair (bp) region spanning the moving forks. Top2 exhibits additional S-phase clusters at specific intergenic loci, mostly containing promoters. TOP1 ablation does not affect fork progression and stability and does not cause activation of the Rad53 checkpoint kinase. top2 mutants accumulate sister chromatid junctions in S phase without affecting fork progression and activate Rad53 at the M-G1 transition. top1 top2 double mutants exhibit fork block and processing and phosphorylation of Rad53 and gamma H2A in S phase. The exonuclease Exo1 influences fork processing and DNA damage checkpoint activation in top1 top2 mutants. Our data are consistent with a coordinated action of Top1 and Top2 in counteracting the accumulation of torsional stress and sister chromatid entanglement at replication forks, thus preventing the diffusion of topological changes along large chromosomal regions. A failure in resolving fork-related topological constrains during S phase may therefore result in abnormal chromosome transitions, DNA damage checkpoint activation, and chromosome breakage during segregation.

  3. Otolith elemental ratios of flathead mullet Mugil cephalus in Taiwanese waters reveal variable patterns of habitat use

    NASA Astrophysics Data System (ADS)

    Wang, C. H.

    2014-12-01

    The migratory history of the flathead mullet (Mugil cephalus) in the coastal waters of Taiwan was evaluated by examining the elemental composition in the otoliths of 74 fish collected from 3 habitats of varying salinity by using laser-ablation inductively coupled plasma mass spectrometry. The mean (±SD) Sr:Ca ratio for the otolith edge differed significantly among the 3 habitats of varying ambient salinity. The otolith mean Sr:Ca ratio for offshore fish was 6.7 ± 2.0 × 10-3 and 4.2 ± 1.5 × 10-3 in the estuary, which was significantly higher than that for freshwater fish (2.8 ± 1.1 × 10-3). By contrast, the mean Ba:Ca ratio for the otolith edge of offshore fish was 87.1 ± 113.0 × 10-6 and 52.1 ± 22.3 × 10-6 in the estuary, which was significantly lower than that for the fish in the freshwater habitat (144.5 ± 54.8 × 10-6). Thus, the Ba:Ca ratio can be used as an alternative to the Sr:Ca ratio for evaluating the migration of M. cephalus between freshwater and saline water. However, the Mn:Ca and Mg:Ca ratios were not significantly different among the 3 habitats. Accordingly, the Sr:Ca and Ba:Ca ratios for the otoliths can be used to reconstruct the salinity history of M. cephalus. The variation in Sr:Ca and Ba:Ca ratios among life history profiles also suggested seasonal migratory behavior in relation to salinity in M. cephalus. These results have implications for developing additional extensive studies to resolve the relative importance of marine estuarine and freshwater habitats for sustaining production of M. cephalus fisheries.

  4. Fort Cobb Reservoir Watershed, Oklahoma and Thika River Watershed, Kenya Twinning Pilot Project

    NASA Astrophysics Data System (ADS)

    Moriasi, D.; Steiner, J.; Arnold, J.; Allen, P.; Dunbar, J.; Shisanya, C.; Gathenya, J.; Nyaoro, J.; Sang, J.

    2007-12-01

    Nairobi. A dam was constructed in 1994 with a water reservoir of 70 million m3. Thika River also supplies water to Masinga Reservoir to supply the seven forks dams, which together supply 75% of the nation's electricity. The quantity of water in rivers and reservoirs is decreased due to sedimentation while water quality is degraded by sediments, and sediment-borne nutrients and pesticides. The focus of this pilot twinning project is watershed erosion and reservoir sedimentation assessment. This will be accomplished by (1) a rapid watershed/catchment erosion assessment using ground based measurements and remote sensing/GIS techniques, 2) use of Acoustic Profiling Systems (APS) for reservoir sedimentation measurement studies, and 3) advanced water quality modeling using the soil and water assessment tool (SWAT) model. Data acquired will be used for sediment transport modeling to1) determine sediment "hot spots" and management practices that will minimize sediments into reservoirs in order to 2) maintain the reservoirs on which many farmers depend for their livelihood and a cleaner environment. This project will provide an opportunity for 1) sharing knowledge and experience among the stakeholders, 2) building capacity through formal and informal education opportunities through reciprocal hosting of decision makers and water experts, and 3) technology transfer of pilot results with recommended management practices to reduce reservoir sedimentation rates.

  5. Scanning Probe Microscopy of DNA with a Quartz Tuning Fork

    NASA Astrophysics Data System (ADS)

    King, G. M.; Nunes, G., Jr.

    2001-03-01

    Quartz tuning-forks have recently been put to use as highly sensitive force detectors in atomic force microscopy (AFM).(F.J.Giessibl et al.), Science 289, 422 (2000). In this study we have applied a home-built, tuning-fork based AFM to the investigation of single and double stranded DNA (ssDNA and dsDNA). We operate the microscope in the non-contact mode (typical tip amplitude ~1 nm) with a variety of tips (e.g. Si, Si_3N_4, W). Here we report on recent results showing that the apparent height of plasmid dsDNA on mica substrates depends on both the tip material and imaging frequency shift. This talk will also review our efforts to probe ssDNA with a chemically functionalized tip. Current and future prospects for this dynamic-mode, chemically-sensitive force microscopy technique will be discussed.

  6. A single-element tuning fork piezoelectric linear actuator.

    PubMed

    Friend, James R; Satonobu, Jun; Nakamura, Kentaro; Ueha, Sadayuki; Stutts, Daniel S

    2003-02-01

    This paper describes the design of a piezoelectric tuning-fork, dual-mode motor. The motor uses a single multilayer piezoelectric element in combination with tuning fork and shearing motion to form an actuator using a single drive signal. Finite-element analysis was used in the design of the motor, and the process is described along with the selection of the device's materials and its performance. Swaging was used to mount the multilayer piezoelectric element within the stator. Prototypes of the 25-mm long bidirectional actuator achieved a maximum linear no-load speed of 16.5 cm/s, a maximum linear force of 1.86 N, and maximum efficiency of 18.9%.

  7. Optimal information provision for maximizing flow in a forked lattice.

    PubMed

    Imai, Takeaki; Nishinari, Katsuhiro

    2015-06-01

    In a forked road, the provision of inappropriate information to car drivers sometimes leads to undesirable situations such as one-sided congestion, which is called the hunting phenomenon in real traffic. To address such problems, we propose a forked exclusion model and investigate the behavior of traffic flow in two routes, providing various types of information to a limited number of traveling particles according to the share rate of information. To analytically understand the phenomena, we develop a coarse-grained representation of the model. By analyzing the model, we find the most effective types of information to minimize particles' travel time and the existence of an optimal share rate according to route conditions.

  8. Optimal information provision for maximizing flow in a forked lattice

    NASA Astrophysics Data System (ADS)

    Imai, Takeaki; Nishinari, Katsuhiro

    2015-06-01

    In a forked road, the provision of inappropriate information to car drivers sometimes leads to undesirable situations such as one-sided congestion, which is called the hunting phenomenon in real traffic. To address such problems, we propose a forked exclusion model and investigate the behavior of traffic flow in two routes, providing various types of information to a limited number of traveling particles according to the share rate of information. To analytically understand the phenomena, we develop a coarse-grained representation of the model. By analyzing the model, we find the most effective types of information to minimize particles' travel time and the existence of an optimal share rate according to route conditions.

  9. Ghana Watershed Prototype Products

    USGS Publications Warehouse

    ,

    2007-01-01

    Introduction/Background A number of satellite data sets are available through the U.S. Geological Survey (USGS) for monitoring land surface features. Representative data sets include Landsat, Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), and Shuttle Radar Topography Mission (SRTM). The Ghana Watershed Prototype Products cover an area within southern Ghana, Africa, and include examples of the aforementioned data sets along with sample SRTM derivative data sets.

  10. Ghana watershed prototype products

    USGS Publications Warehouse

    ,

    2007-01-01

    A number of satellite data sets are available through the U.S. Geological Survey (USGS) for monitoring land surface features. Representative data sets include Landsat, Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), and Shuttle Radar Topography Mission (SRTM). The Ghana Watershed Prototype Products cover an area within southern Ghana, Africa, and include examples of the aforementioned data sets along with sample SRTM derivative data sets.

  11. South Fork Clearwater River Habitat Enhancement, Nez Perce National Forest.

    SciTech Connect

    Siddall, Phoebe

    1992-04-01

    In 1984, the Nez Perce National forest and the Bonneville Power Administration entered into a contractual agreement which provided for improvement of spring chinook salmon and summer steelhead trout habitat in south Fork Clearwater River tributaries. Project work was completed in seven main locations: Crooked River, Red River, Meadow Creek Haysfork Gloryhole, Cal-Idaho Gloryhole, Fisher Placer and Leggett Placer. This report describes restoration activities at each of these sites.

  12. Environmental Assessment: Demolish 452 at Grand Forks Air Force Base

    DTIC Science & Technology

    2005-12-01

    humid with frequent thunderstorms. Winters are long and severe with almost continuous snow cover. The spring and fall seasons are generally short...direction is generally from the northwest during the late fall, winter, and spring , and from the southeast during the summer. Grand Forks County is...to Lake Winnipeg, Canada. The Red River drainage basin is part of the Hudson Bay drainage system. At Manvel, ND, approximately 10 miles northeast

  13. Geomorphological Analysis of North Fork, Toutle River, Washington: 1980- 1984

    DTIC Science & Technology

    1994-03-01

    north flank of Mount St. Helens and included glacial ice, modern dacite dome, and a mixture of andesite and minor amounts of basalt lava flows...and basalt form the former domes. During emplacement this deposit also consisted of snow and large blocks of ice from the glaciers on the north flank of...north side of Mount St. Helens. Consequently,the North Fork Unit features as its dominant 23 materials the dark colored mixed basalt deposits and the

  14. Environmental Assessment Housing Transfer at Grand Forks AFB, North Dakota

    DTIC Science & Technology

    2005-01-27

    tallgrass prairie that once was dominant in this region. Plants thriving in this preserve include western wheatgrass, slender wheatgrass, big bluestem...Palustrine Forested type wetlands. Vegetation is robust at GFAFB wetlands, and they are characterized as typical prairie potholes found within the...grassland and wetland habitats for wildlife in Grand Forks County. Pastures, meadows, and other non-cultivated areas create a prairie -land mosaic

  15. Recent Approaches to Modeling Transport of Mercury in Surface Water and Groundwater - Case Study in Upper East Fork Poplar Creek, Oak Ridge, TN - 13349

    SciTech Connect

    Bostick, Kent; Daniel, Anamary; Tachiev, Georgio; Malek-Mohammadi, Siamak

    2013-07-01

    In this case study, groundwater/surface water modeling was used to determine efficacy of stabilization in place with hydrologic isolation for remediation of mercury contaminated areas in the Upper East Fork Poplar Creek (UEFPC) Watershed in Oak Ridge, TN. The modeling simulates the potential for mercury in soil to contaminate groundwater above industrial use risk standards and to contribute to surface water contamination. The modeling approach is unique in that it couples watershed hydrology with the total mercury transport and provides a tool for analysis of changes in mercury load related to daily precipitation, evaporation, and runoff from storms. The model also allows for simulation of colloidal transport of total mercury in surface water. Previous models for the watershed only simulated average yearly conditions and dissolved concentrations that are not sufficient for predicting mercury flux under variable flow conditions that control colloidal transport of mercury in the watershed. The transport of mercury from groundwater to surface water from mercury sources identified from information in the Oak Ridge Environmental Information System was simulated using a watershed scale model calibrated to match observed daily creek flow, total suspended solids and mercury fluxes. Mercury sources at the former Building 81-10 area, where mercury was previously retorted, were modeled using a telescopic refined mesh with boundary conditions extracted from the watershed model. Modeling on a watershed scale indicated that only source excavation for soils/sediment in the vicinity of UEFPC had any effect on mercury flux in surface water. The simulations showed that colloidal transport contributed 85 percent of the total mercury flux leaving the UEFPC watershed under high flow conditions. Simulation of dissolved mercury transport from liquid elemental mercury and adsorbed sources in soil at former Building 81-10 indicated that dissolved concentrations are orders of magnitude

  16. Replication patterns and organization of replication forks in Vibrio cholerae.

    PubMed

    Stokke, Caroline; Waldminghaus, Torsten; Skarstad, Kirsten

    2011-03-01

    We have investigated the replication patterns of the two chromosomes of the bacterium Vibrio cholerae grown in four different media. By combining flow cytometry and quantitative real-time PCR with computer simulations, we show that in rich media, V. cholerae cells grow with overlapping replication cycles of both the large chromosome (ChrI) and the small chromosome (ChrII). In Luria-Bertani (LB) medium, initiation occurs at four copies of the ChrI origin and two copies of the ChrII origin. Replication of ChrII was found to occur at the end of the ChrI replication period in all four growth conditions. Novel cell-sorting experiments with marker frequency analysis support these conclusions. Incubation with protein synthesis inhibitors indicated that the potential for initiation of replication of ChrII was present at the same time as that of ChrI, but was actively delayed until much of ChrI was replicated. Investigations of the localization of SeqA bound to new DNA at replication forks indicated that the forks were co-localized in pairs when cells grew without overlapping replication cycles and in higher-order structures during more rapid growth. The increased degree of fork organization during rapid growth may be a means by which correct segregation of daughter molecules is facilitated.

  17. EPA'S WATERSHED MANAGEMENT AND MODELING RESEARCH PROGRAM

    EPA Science Inventory

    Watershed management presumes that community groups can best solve many water quality and ecosystem problems at the watershed level rather than at the individual site, receiving waterbody, or discharger level. After assessing and ranking watershed problems, and setting environ...

  18. RESEARCH NEEDS FOR EFFECTIVE WATERSHED PLANNING

    EPA Science Inventory

    Watershed research has historically focused on physical and biological processes, stressor-response, and effects research, providing valuable understanding of the effects of human activity and natural disturbances on watershed ecosystems. Continued research to support watershed ...

  19. Human RECQ1 promotes restart of replication forks reversed by DNA topoisomerase I inhibition.

    PubMed

    Berti, Matteo; Ray Chaudhuri, Arnab; Thangavel, Saravanabhavan; Gomathinayagam, Shivasankari; Kenig, Sasa; Vujanovic, Marko; Odreman, Federico; Glatter, Timo; Graziano, Simona; Mendoza-Maldonado, Ramiro; Marino, Francesca; Lucic, Bojana; Biasin, Valentina; Gstaiger, Matthias; Aebersold, Ruedi; Sidorova, Julia M; Monnat, Raymond J; Lopes, Massimo; Vindigni, Alessandro

    2013-03-01

    Topoisomerase I (TOP1) inhibitors are an important class of anticancer drugs. The cytotoxicity of TOP1 inhibitors can be modulated by replication fork reversal through a process that requires poly(ADP-ribose) polymerase (PARP) activity. Whether regressed forks can efficiently restart and what factors are required to restart fork progression after fork reversal are still unknown. We have combined biochemical and EM approaches with single-molecule DNA fiber analysis to identify a key role for human RECQ1 helicase in replication fork restart after TOP1 inhibition that is not shared by other human RecQ proteins. We show that the poly(ADP-ribosyl)ation activity of PARP1 stabilizes forks in the regressed state by limiting their restart by RECQ1. These studies provide new mechanistic insights into the roles of RECQ1 and PARP in DNA replication and offer molecular perspectives to potentiate chemotherapeutic regimens based on TOP1 inhibition.

  20. Human RECQ1 promotes restart of replication forks reversed by DNA topoisomerase I inhibition

    PubMed Central

    Berti, Matteo; Chaudhuri, Arnab Ray; Thangavel, Saravanabhavan; Gomathinayagam, Shivasankari; Kenig, Sasa; Vujanovic, Marko; Odreman, Federico; Glatter, Timo; Graziano, Simona; Mendoza-Maldonado, Ramiro; Marino, Francesca; Lucic, Bojana; Biasin, Valentina; Gstaiger, Matthias; Aebersold, Ruedi; Sidorova, Julia M.; Monnat, Raymond J.; Lopes, Massimo; Vindigni, Alessandro

    2014-01-01

    SUMMARY Topoisomerase I (TOP1) inhibitors are an important class of anticancer drugs. The cytotoxicity of TOP1 inhibitors can be modulated by replication fork reversal, in a process that requires PARP activity. Whether regressed forks can efficiently restart and the factors required to restart fork progression after fork reversal are still unknown. Here we combined biochemical and electron microscopy approaches with single-molecule DNA fiber analysis, to identify a key role for human RECQ1 helicase in replication fork restart after TOP1 inhibition, not shared by other human RecQ proteins. We show that the poly(ADPribosyl)ation activity of PARP1 stabilizes forks in their regressed state by limiting their restart by RECQ1. These studies provide new mechanistic insights into the roles of RECQ1 and PARP in DNA replication and offer molecular perspectives to potentiate chemotherapeutic regimens based on TOP1 inhibition. PMID:23396353

  1. High-resolution digital elevation model of Mount St. Helens crater and upper North Fork Toutle River basin, Washington, based on an airborne lidar survey of September 2009

    USGS Publications Warehouse

    Mosbrucker, Adam

    2014-01-01

    The lateral blast, debris avalanche, and lahars of the May 18th, 1980, eruption of Mount St. Helens, Washington, dramatically altered the surrounding landscape. Lava domes were extruded during the subsequent eruptive periods of 1980–1986 and 2004–2008. More than three decades after the emplacement of the 1980 debris avalanche, high sediment production persists in the North Fork Toutle River basin, which drains the northern flank of the volcano. Because this sediment increases the risk of flooding to downstream communities on the Toutle and Cowlitz Rivers, the U.S. Army Corps of Engineers (USACE), under the direction of Congress to maintain an authorized level of flood protection, built a sediment retention structure on the North Fork Toutle River in 1989 to help reduce this risk and to prevent sediment from clogging the shipping channel of the Columbia River. From September 16–20, 2009, Watershed Sciences, Inc., under contract to USACE, collected high-precision airborne lidar (light detection and ranging) data that cover 214 square kilometers (83 square miles) of Mount St. Helens and the upper North Fork Toutle River basin from the sediment retention structure to the volcano's crater. These data provide a digital dataset of the ground surface, including beneath forest cover. Such remotely sensed data can be used to develop sediment budgets and models of sediment erosion, transport, and deposition. The U.S. Geological Survey (USGS) used these lidar data to develop digital elevation models (DEMs) of the study area. DEMs are fundamental to monitoring natural hazards and studying volcanic landforms, fluvial and glacial geomorphology, and surface geology. Watershed Sciences, Inc., provided files in the LASer (LAS) format containing laser returns that had been filtered, classified, and georeferenced. The USGS produced a hydro-flattened DEM from ground-classified points at Castle, Coldwater, and Spirit Lakes. Final results averaged about five laser last

  2. Validation of the Soil Moisture Active Passive mission using USDA-ARS experimental watersheds

    NASA Astrophysics Data System (ADS)

    Cosh, M. H.; Jackson, T. J.; Bindlish, R.; Colliander, A.; Kim, S.; Das, N. N.; Yueh, S. H.; Bosch, D. D.; Goodrich, D. C.; Prueger, J. H.; Starks, P. J.; Livingston, S.; Seyfried, M. S.; Coopersmith, E. J.

    2015-12-01

    The calibration and validation program of the Soil Moisture Active Passive mission (SMAP) relies upon an international cooperative of in situ networks to provide ground truth references across a variety of landscapes. The USDA Agricultural Research Service operates several experimental watersheds which contribute to the validation of SMAP soil moisture products. These watersheds consist of a network of in situ sensors that measure soil moisture at a variety of depths including the 5 cm depth, which is critical for satellite validation. Comparisons of the in situ network estimates to the satellite products are ongoing, but initial results have shown strong correlation between satellite estimates and in situ soil moisture measurements once scaling functions were applied. The scaling methodologies for the in situ networks are being reviewed and evaluated. Results from the Little Washita, Fort Cobb, St. Joseph's and Little River Experimental Watersheds show good agreement between the satellite products and in situ measurements. Walnut Gulch results show high accuracy, although with the caveat that these domains are semi-arid with a substantially lower dynamic range. The South Fork Watershed is examined more closely for its detailed scaling function development as well as an apparent bias between satellite and in situ values.

  3. Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells

    PubMed Central

    Zellweger, Ralph; Dalcher, Damian; Mutreja, Karun; Berti, Matteo; Schmid, Jonas A.; Herrador, Raquel; Vindigni, Alessandro

    2015-01-01

    Replication fork reversal protects forks from breakage after poisoning of Topoisomerase 1. We here investigated fork progression and chromosomal breakage in human cells in response to a panel of sublethal genotoxic treatments, using other topoisomerase poisons, DNA synthesis inhibitors, interstrand cross-linking inducers, and base-damaging agents. We used electron microscopy to visualize fork architecture under these conditions and analyzed the association of specific molecular features with checkpoint activation. Our data identify replication fork uncoupling and reversal as global responses to genotoxic treatments. Both events are frequent even after mild treatments that do not affect fork integrity, nor activate checkpoints. Fork reversal was found to be dependent on the central homologous recombination factor RAD51, which is consistently present at replication forks independently of their breakage, and to be antagonized by poly (ADP-ribose) polymerase/RECQ1-regulated restart. Our work establishes remodeling of uncoupled forks as a pivotal RAD51-regulated response to genotoxic stress in human cells and as a promising target to potentiate cancer chemotherapy. PMID:25733714

  4. The fork and the kinase: a DNA replication tale from a CHK1 perspective.

    PubMed

    González Besteiro, Marina A; Gottifredi, Vanesa

    2015-01-01

    Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. Checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged DNA. Subsequently, Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Indeed, such findings have unveiled a puzzling connection between Chk1 and DNA lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, Chk1 is a multifaceted and versatile signaling factor that acts at ongoing forks and replication origins to determine the extent and quality of the cellular response to replication stress.

  5. Navigating the socio-bio-geo-chemistry and engineering of nitrogen management in two illinois tile-drained watersheds.

    PubMed

    David, Mark B; Flint, Courtney G; Gentry, Lowell E; Dolan, Mallory K; Czapar, George F; Cooke, Richard A; Lavaire, Tito

    2015-03-01

    Reducing nitrate loads from corn and soybean, tile-drained, agricultural production systems in the Upper Mississippi River basin is a major challenge that has not been met. We evaluated a range of possible management practices from biophysical and social science perspectives that could reduce nitrate losses from tile-drained fields in the Upper Salt Fork and Embarras River watersheds of east-central Illinois. Long-term water quality monitoring on these watersheds showed that nitrate losses averaged 30.6 and 23.0 kg nitrate N ha yr (Embarras and Upper Salt Fork watersheds, respectively), with maximum nitrate concentrations between 14 and 18 mg N L. With a series of on-farm studies, we conducted tile monitoring to evaluate several possible nitrate reduction conservation practices. Fertilizer timing and cover crops reduced nitrate losses (30% reduction in a year with large nitrate losses), whereas drainage water management on one tile system demonstrated the problems with possible retrofit designs (water flowed laterally from the drainage water management tile to the free drainage system nearby). Tile woodchip bioreactors had good nitrate removal in 2012 (80% nitrate reduction), and wetlands had previously been shown to remove nitrate (45% reductions) in the Embarras watershed. Interviews and surveys indicated strong environmental concern and stewardship ethics among landowners and farmers, but the many financial and operational constraints that they operate under limited their willingness to adopt conservation practices that targeted nitrate reduction. Under the policy and production systems currently in place, large-scale reductions in nitrate losses from watersheds such as these in east-central Illinois will be difficult.

  6. Riverine Response of Sulfate to Declining Atmospheric Sulfur Deposition in Agricultural Watersheds.

    PubMed

    David, Mark B; Gentry, Lowell E; Mitchell, Corey A

    2016-07-01

    Sulfur received extensive study as an input to terrestrial ecosystems from acidic deposition during the 1980s. With declining S deposition inputs across the eastern United States, there have been many studies evaluating ecosystem response, with the exception of agricultural watersheds. We used long-term (22 and 18 yr) sulfate concentration data from two rivers and recent (6 yr) data from a third river to better understand cycling and transport of S in agricultural, tile-drained watersheds. Sulfate concentrations and yields steadily declined in the Embarras (from ∼10 to 6 mg S L) and Kaskaskia rivers (from 7 to 3.5 mg S L) during the sampling period, with an overall -23.1 and -12.8 kg S ha yr balance for the two watersheds. There was evidence of deep groundwater inputs of sulfate in the Salt Fork watershed, with a much smaller input to the Embarras and none to the Kaskaskia. Tiles in the watersheds had low sulfate concentrations (<10 mg S L), similar to the Kaskaskia River, unless the field had received some form of S fertilizer. A multiple regression model of runoff (cm) and S deposition explained much of the variation in Embarras River sulfate ( = 0.86 and 0.80 for concentrations and yields; = 46). Although atmospheric deposition was much less than outputs (grain harvest + stream export of sulfate), riverine transport of sulfate reflected the decline in inputs. Watershed S balances suggest a small annual depletion of soil organic S pools, and S fertilization will likely be needed at some future date to maintain crop yields.

  7. Evaluating the effectiveness of floodplain restoration on the North Fork John Day River, Northeast Oregon, USA

    NASA Astrophysics Data System (ADS)

    Clifton, C. F.; Blanton, P.; Long, W.; Walterman, M. T.; McDowell, P. F.; Maus, P.

    2007-12-01

    Over the last decade hundreds of river restoration projects intended to maintain, protect, and restore watersheds, rivers, and habitat for native species in the Pacific Northwest have been implemented. By some counts, investment in watershed restoration exceeds hundreds of millions of dollars annually yet the effectiveness of these efforts remains an elusive question (Roni, 2005). Remote sensing and GIS technologies show great promise for large-scale river monitoring, however most natural resource organizations who implement these projects have limited budget and staff and would benefit from simple, low cost monitoring techniques that use readily available imagery. We used 1:24000 digitized orthorectified resource imagery from 1995, and National Agriculture Imagery Program (NAIP) digital orthophotography from 2005 to assess the effectiveness of floodplain restoration on a 16 km reach of the North Fork John Day River. Between 1993 and 1997 this section was restored by mechanically removing, reshaping, and revegetating cobble-boulder tailings piles left from dredge mining. The project was intended to directly improve floodplain function (i.e. inundation, riparian habitat) and indirectly improve instream habitat (pools, spawning) by reconnecting the active river channel with a reconstructed floodplain surface. Project effectiveness was not well documented initially in terms of quantifying floodplain functional area improvement or channel condition and response at the river-reach scale. Our objectives were to field-verify remote sensing measurements of response variables to test the applicability of available remote sensing imagery for project effectiveness monitoring, and to quantify adjustment in river response variables, using a "before-after" case study approach. Bracketing restoration activities with 1995 and 2000 imagery, we developed and tested methods for acquisition and processing of digital imagery and identified a core set of response variables to sample

  8. Geomorphic Effects of Engineered Log Jams in River Restoration, Middle Fork John Day River

    NASA Astrophysics Data System (ADS)

    Duffin, J.; McDowell, P. F.

    2014-12-01

    The Middle Fork of the John Day River (MFJD) Intensively Monitored Watershed in eastern Oregon is a multi-phase restoration implementation and monitoring project. MFJD is a tributary to the Colombia and is part of one of the longest free flowing rivers systems in the continental United States. It is a gravel and cobble bed river with a drainage area of 2,100 km2. The river has endured extensive channel and floodplain degradation from years of channel alteration and straightening due to human influences including dredge mining, ranching, and farming. As part of the river restoration project on the MFJD, engineered log jams have been constructed to address many of the restoration goals including creating scour pools, inhibiting bank erosion, creating and maintaining a sinuous river planform, and increasing complexity of fish habitat. There is a need for more detailed understanding on ELJ channel morphologic effects and how site-specific characteristics and differences in log jam infrastructure interact to create the in-channel features over timescales longer than a few years. This study uses detailed channel bed topographic surveys collected either with a total station or RTK-GPS technology. Geomorphic change detection techniques are utilized to monitor topographic change under and around the 26 log structures in two different river reaches over a six to seven year period The log structures are often associated with deepening of pools as desired, but also some structures show sedimentation under the structure. Differences in the patterns will be assessed based on the design, location, and specific characteristics of the log structures; variables include number and placement of logs, volume of structure, location on meander bend, and sediment sizes.

  9. INTEGRATIVE CONSIDERATIONS IN WATERSHED PLANNING

    EPA Science Inventory

    Understanding the filters through which society views the values produced by watersheds is key to developing effective and adaptable watershed plans, and ultimately a measure of how well policy makers are likely to meet a sustainability, or any other, intent. Many natural resour...

  10. Watershed Education for Sustainable Development.

    ERIC Educational Resources Information Center

    Stapp, William B.

    2000-01-01

    Presents information on the Global Rivers Environmental Education Network (GREEN), which is a global communication system for analyzing watershed usage and monitoring the quality and quantity of river water. Describes GREEN's watershed educational model and strategies and international development. (Contains 67 references.) (Author/YDS)

  11. MIDDLE FORK OF THE JUDITH RIVER WILDERNESS STUDY AREA, MONTANA.

    USGS Publications Warehouse

    Reynolds, Mitchell W.; Hamilton, Michael

    1984-01-01

    The Middle Fork of the Judith River Wilderness Study Area in Montana has several prospects containing local small inferred resources and several exposed mineral occurrences bearing silver, lead, copper, and gold along its north and west edges with no identified resource potential. Geologic, geophysical, and geochemical studies, conducted to appraise the mineral resources of the study area, identified no other anomalous concentrations of metallic elements. A gem-quality sapphire resource, present near the east boundary, cannot be identified within the study area. The area holds little promise for the occurrence of other mineral or energy resources.

  12. Potential Impacts of Climate Change on Streamflow and Flooding in Snow Dominated Forest Basins

    SciTech Connect

    Wigmosta, Mark S.; Leung, Lai R.

    2003-01-01

    Changes in climate resulting from the buildup of greenhouse gases in the atmosphere may impact significantly the frequency and magnitude of flooding in forested watersheds through changes in snowpack, soil moisture, and runoff production. The level of impact will vary considerably among watersheds depending on the climate regime and hydrologic characteristics of the catchments. Two forested watersheds in the Pacific Northwest of the United States, the American River and Middle Fork Flathead River, were studied to examine the influence of long-term global warming on streamflow and flooding in snowmelt dominated basins. These watersheds were selected to compare impacts resulting from changes in the maritime and continental climate regimes associated with the American and Middle Fork Flathead River basins, respectively. Output from a regional climate model was used to drive a distributed hydrologic model under present and future climate conditions corresponding to a doubling of atmospheric CO2. Under the future climate scenario more winter precipitation fell as rain instead of snow producing higher winter flows, a reduced snowpack, and decreased spring and summer flows in the American River. In addition, there was a large increase in the frequency and magnitude of winter flooding, primarily due to an increase in the number of rain-on-snow events. The change was much less dramatic in the Middle Fork Flathead River where flooding generally occurs during spring snowmelt. In this basin, the seasonal pattern of streamflow remains intact and the incidence of flooding was reduced under the future climate scenario. This study suggests the impacts of climate change on streamflow and flooding in forested watersheds are highly region specific.

  13. Remedial investigation work plan for the Upper East Fork Poplar Creek characterization area, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    SciTech Connect

    1995-09-01

    The Oak Ridge Y-12 Plant, located within the Oak Ridge Reservation (ORR), is owned by the US Department of Energy (DOE) and managed by Lockheed Martin Energy Systems, Inc. The entire ORR was placed on the National Priorities List (NPL) of CERCLA sites in November 1989. Following CERCLA guidelines, sites under investigation require a remedial investigation (RI) to define the nature and extent of contamination, evaluate the risks to public health and the environment, and determine the goals for a feasibility study (FS) of potential remedial actions. The need to complete RIs in a timely manner resulted in the establishment of the Upper East Fork Poplar Creek (UEFPC) Characterization Area (CA) and the Bear Creek CA. The CA approach considers the entire watershed and examines all appropriate media within it. The UEFPC CA, which includes the main Y-12 Plant area, is an operationally and hydrogeologically complex area that contains numerous contaminants and containment sources, as well as ongoing industrial and defense-related activities. The UEFPC CA also is the suspected point of origin for off-site groundwater and surface-water contamination. The UEFPC CA RI also will address a carbon-tetrachloride/chloroform-dominated groundwater plume that extends east of the DOE property line into Union Valley, which appears to be connected with springs in the valley. In addition, surface water in UEFPC to the Lower East Fork Poplar Creek CA boundary will be addressed. Through investigation of the entire watershed as one ``site,`` data gaps and contaminated areas will be identified and prioritized more efficiently than through separate investigations of many discrete units.

  14. 78 FR 48292 - Amendment of Class D Airspace; Grand Forks AFB, ND

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class D Airspace; Grand Forks AFB, ND AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class D... Federal Register a notice of proposed rulemaking (NPRM) to amend Class D airspace for Grand Forks AFB,...

  15. HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.

    PubMed

    Kile, Andrew C; Chavez, Diana A; Bacal, Julien; Eldirany, Sherif; Korzhnev, Dmitry M; Bezsonova, Irina; Eichman, Brandt F; Cimprich, Karlene A

    2015-06-18

    Stalled replication forks are a critical problem for the cell because they can lead to complex genome rearrangements that underlie cell death and disease. Processes such as DNA damage tolerance and replication fork reversal protect stalled forks from these events. A central mediator of these DNA damage responses in humans is the Rad5-related DNA translocase, HLTF. Here, we present biochemical and structural evidence that the HIRAN domain, an ancient and conserved domain found in HLTF and other DNA processing proteins, is a modified oligonucleotide/oligosaccharide (OB) fold that binds to 3' ssDNA ends. We demonstrate that the HIRAN domain promotes HLTF-dependent fork reversal in vitro through its interaction with 3' ssDNA ends found at forks. Finally, we show that HLTF restrains replication fork progression in cells in a HIRAN-dependent manner. These findings establish a mechanism of HLTF-mediated fork reversal and provide insight into the requirement for distinct fork remodeling activities in the cell.

  16. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... National Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the...] Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park... visitor services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not...

  17. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork Red River, Okla. The Bureau of Reclamation, or its designated agent, shall operate the Altus... of runoff from the area above the dam exceeds the volume of water necessary to raise the...

  18. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork Red River, Okla. The Bureau of Reclamation, or its designated agent, shall operate the Altus... of runoff from the area above the dam exceeds the volume of water necessary to raise the...

  19. First report of anther smut caused by Microbotryum violaceum on forked catchfly in Turkey

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Forked catchfly (Silene dichotoma Ehrh.), family Caryophyllaceae, is a common and native plant in rangelands and pastures in Turkey. It is also an introduced plant that is widely distributed in North America. In May, 2007 about 20 forked catchfly plants on the campus of Ondokuz Mayis University, i...

  20. Origin & Evolution of the Grand Forks Human Nutrition Research Center, 1970-90

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the early 1960s William E Cornatzer, MD, PhD, suggested the need for increased USDA research concerning human nutrition, and creation of the Grand Forks Human Nutrition Laboratory (Grand Forks Human Nutrition Research Center). He shared ideas with Senator Milton R. Young of North Dakota, who requ...

  1. Watershed-based survey designs

    USGS Publications Warehouse

    Detenbeck, N.E.; Cincotta, D.; Denver, J.M.; Greenlee, S.K.; Olsen, A.R.; Pitchford, A.M.

    2005-01-01

    Watershed-based sampling design and assessment tools help serve the multiple goals for water quality monitoring required under the Clean Water Act, including assessment of regional conditions to meet Section 305(b), identification of impaired water bodies or watersheds to meet Section 303(d), and development of empirical relationships between causes or sources of impairment and biological responses. Creation of GIS databases for hydrography, hydrologically corrected digital elevation models, and hydrologic derivatives such as watershed boundaries and upstream–downstream topology of subcatchments would provide a consistent seamless nationwide framework for these designs. The elements of a watershed-based sample framework can be represented either as a continuous infinite set defined by points along a linear stream network, or as a discrete set of watershed polygons. Watershed-based designs can be developed with existing probabilistic survey methods, including the use of unequal probability weighting, stratification, and two-stage frames for sampling. Case studies for monitoring of Atlantic Coastal Plain streams, West Virginia wadeable streams, and coastal Oregon streams illustrate three different approaches for selecting sites for watershed-based survey designs.

  2. Watershed-based survey designs.

    PubMed

    Detenbeck, Naomi E; Cincotta, Dan; Denver, Judith M; Greenlee, Susan K; Olsen, Anthony R; Pitchford, Ann M

    2005-04-01

    Watershed-based sampling design and assessment tools help serve the multiple goals for water quality monitoring required under the Clean Water Act, including assessment of regional conditions to meet Section 305(b), identification of impaired water bodies or watersheds to meet Section 303(d), and development of empirical relationships between causes or sources of impairment and biological responses. Creation of GIS databases for hydrography, hydrologically corrected digital elevation models, and hydrologic derivatives such as watershed boundaries and upstream-downstream topology of subcatchments would provide a consistent seamless nationwide framework for these designs. The elements of a watershed-based sample framework can be represented either as a continuous infinite set defined by points along a linear stream network, or as a discrete set of watershed polygons. Watershed-based designs can be developed with existing probabilistic survey methods, including the use of unequal probability weighting, stratification, and two-stage frames for sampling. Case studies for monitoring of Atlantic Coastal Plain streams, West Virginia wadeable streams, and coastal Oregon streams illustrate three different approaches for selecting sites for watershed-based survey designs.

  3. Faunal assemblages and multi-scale habitat patterns in headwater tributaries of the South Fork Trinity River - an unregulated river embedded within a multiple-use landscape

    USGS Publications Warehouse

    Welsh, H.H.; Hodgson, G.R.; Duda, J.J.; Emlen, J.M.

    2010-01-01

    Headwaters can represent 80% of stream kilometers in a watershed, and they also have unique physical and biological properties that have only recently been recognized for their importance in sustaining healthy functioning stream networks and their ecological services. We sampled 60 headwater tributaries in the South Fork Trinity River, a 2,430 km2, mostly forested, multiple-use watershed in northwestern California. Our objectives were: (1) to differentiate unique headwater types using 69 abiotic and vegetation variables measured at three spatial scales, and then to reduce these to informative subsets; (2) determine if distinct biota occupied the different tributary types; (3) determine the environmental attributes associated with the presence and abundance of these biotic assemblages; and (4) using niche modeling, determine key attribute thresholds to illustrate how these biota could be employed as metrics of system integrity and ecological services. Several taxa were sufficiently abundant and widespread to use as bio-indicators: the presence and abundance of steelhead trout (Oncorhynchus mykiss), herpetofauna (reptile and amphibian) species richness, and signal crayfish (Pacifastacus leniusculus) represented different trophic positions, value as commercial resources (steelhead), sensitivity to environmental stress (amphibians), and indicators of biodiversity (herpetofauna species richness). Herpetofauna species richness did not differ, but abundances of steelhead trout, signal crayfish, and amphibian richness all differed significantly among tributary types. Niche models indicated that distribution and abundance patterns in both riparian and aquatic environments were associated with physical and structural attributes at multiple spatial scales, both within and around reaches. The bio-indicators responded to unique sets of attributes, reflecting the high environmental heterogeneity in headwater tributaries across this large watershed. These niche attributes

  4. Active Control of Repetitive Structural Transitions between Replication Forks and Holliday Junctions by Werner Syndrome Helicase

    PubMed Central

    Shin, Soochul; Lee, Jinwoo; Yoo, Sangwoon; Kulikowicz, Tomasz; Bohr, Vilhelm A.; Ahn, Byungchan; Hohng, Sungchul

    2016-01-01

    SUMMARY The reactivation of stalled DNA replication via fork regression invokes Holliday junction formation, branch migration, and the recovery of the replication fork after DNA repair or error-free DNA synthesis. The coordination mechanism for these DNA structural transitions by molecular motors, however, remains unclear. Here we perform single-molecule fluorescence experiments with Werner syndrome protein (WRN) and model replication forks. The Holliday junction is readily formed once the lagging arm is unwound, and migrated unidirectionally with 3.2 ± 0.03 bases/s velocity. The recovery of the replication fork was controlled by branch migration reversal of WRN, resulting in repetitive fork regression. The Holliday junction formation, branch migration, and migration direction reversal are all ATP dependent, revealing that WRN uses the energy of ATP hydrolysis to actively coordinate the structural transitions of DNA. PMID:27427477

  5. 77 FR 64125 - Notice of Proposed Withdrawal Extension and Notification of a Public Meeting for the East Fork...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-18

    ... for the East Fork Elk Winter Range; WY AGENCY: Bureau of Land Management, Interior. ACTION: Notice... States mining laws, to protect the East Fork Elk Winter Range and elk natural feeding grounds in Fremont... mineral estate from location or entry under the United States mining laws, to protect the East Fork...

  6. 59 FR- Prohibited Acts in West Little and North Fork Owyhee National Wild and Scenic River Area

    Federal Register 2010, 2011, 2012, 2013, 2014

    1994-12-15

    ... INTERIOR [OR-030-03-1220-04: GS-043] Prohibited Acts in West Little and North Fork Owyhee National Wild and... and restrictions within the boundaries of the West Little and North Fork Owyhee Rivers as established in the Main, West Little and North Fork Owyhee National Wild and Scenic Rivers Management...

  7. Depositional environments, reservoir trends, and diagenesis of Red Fork sandstones in parts of Blaine, Caddo, and Custer counties, Oklahoma

    SciTech Connect

    Johnson, C.L.

    1984-04-01

    The Red Fork sandstone was divided into the upper and lower Red Fork which are separated by a consistent marker bed. The Red Fork interval thickens markedly across the study area from 250 ft (75 m) in the northeast to over 1300 ft (400 m) in the southwest. Most of the thickening is within the lower Red Fork. The lower Red Fork is believed to have been deposited in shelf-to-basin transitional terrain. Sands were located in delta-front, submarine-channel-fill, and possible submarine-fan terrain. The upper Red Fork is believed to represent the maximum progradation of a deltaic complex. Sandstones of the lower Red Fork are sublithic to lithic arenites; the upper Red Fork is sublithic arenite. The dominant lithic fraction is mudstone fragments. The main diagenetic alterations of both the upper and lower Red Fork sandstones were destruction of primary porosity by compaction and cementation. Dissolution chiefly of mud fragments has produced well-developed secondary porosity. Clays of the lower Red Fork mainly are authigenic chlorite; clays of the upper Red Fork primarily are authigenic kaolinite. Present oil and gas production from Red Fork sandstones is most abundant from localities on the paleoshelf.

  8. Tracking Sediment Movement with Pittag Surveys during Storm Events in the North Fork of Caspar Creek, California

    NASA Astrophysics Data System (ADS)

    Leidman, S. Z.; Yarnell, S. M.; Yager, E.

    2015-12-01

    Caspar Creek is a gravel-bedded stream near Fort Bragg in Jackson Demonstration State Forest in Northern California's Coast Range. Since 1962, detailed measurements of streamflow, turbidity, and rainfall have been made at a weir just below the study area by the Pacific Southwest Research Station and California Department of Forestry and Fire Protection (CAL FIRE). However, little research has been done to identify how gravel-sized sediment moves through the system during storm flows. The North Fork of Caspar Creek drains a relatively small and steep watershed providing fast residence times that allow direct correlation between rainfall events and movement of the gravel-dominated (<20% sand) bed material. The study reach has an average discharge of 3.8x10-2 m3/s, yet over the past two years has had 4 storm events with flows greater than 1.0 m3/s, a flow that has shown to be a minimum needed for bedload movement. Gravel sized rocks inserted with HDX pit tags were placed throughout the study reach and surveyed after each storm to track their movement through the system. The results show that bed movement responds nonlinearly to increased peak storm discharge with larger storms disproportionately moving sediment further downstream. The movement distances appear independent of local slope, and the distribution of transported sediment sizes does not show significant variance between different storm events. This info will contribute to a better understanding of gravel deposition and bar formation in rainfall-driven watersheds and may help predict sediment fluxes during storm events.

  9. Watersheds and Explosive percolation

    NASA Astrophysics Data System (ADS)

    Herrmann, Hans J.; Araujo, Nuno A. M.

    The recent work by Achlioptas, D'Souza, and Spencer opened up the possibility of obtaining a discontinuous (explosive) percolation transition by changing the stochastic rule of bond occupation. Despite the active research on this subject, several questions still remain open about the leading mechanism and the properties of the system. We review the largest cluster and the Gaussian models recently introduced. We show that, to obtain a discontinuous transition it is solely necessary to control the size of the largest cluster, suppressing the growth of a cluster di_ering significantly, in size, from the average one. As expected for a discontinuous transition, a Gaussian cluster-size distribution and compact clusters are obtained. The surface of the clusters is fractal, with the same fractal dimension of the watershed line.

  10. CtIP mediates replication fork recovery in a FANCD2-regulated manner.

    PubMed

    Yeo, Jung Eun; Lee, Eu Han; Hendrickson, Eric A; Sobeck, Alexandra

    2014-07-15

    Fanconi anemia (FA) is a chromosome instability syndrome characterized by increased cancer predisposition. Within the FA pathway, an upstream FA core complex mediates monoubiquitination and recruitment of the central FANCD2 protein to sites of stalled replication forks. Once recruited, FANCD2 fulfills a dual role towards replication fork recovery: (i) it cooperates with BRCA2 and RAD51 to protect forks from nucleolytic degradation and (ii) it recruits the BLM helicase to promote replication fork restart while suppressing new origin firing. Intriguingly, FANCD2 and its interaction partners are also involved in homologous recombination (HR) repair of DNA double-strand breaks, hinting that FANCD2 utilizes HR proteins to mediate replication fork recovery. One such candidate is CtIP (CtBP-interacting protein), a key HR repair factor that functions in complex with BRCA1 and MRE11, but has not been investigated as putative player in the replication stress response. Here, we identify CtIP as a novel interaction partner of FANCD2. CtIP binds and stabilizes FANCD2 in a DNA damage- and FA core complex-independent manner, suggesting that FANCD2 monoubiquitination is dispensable for its interaction with CtIP. Following cellular treatment with a replication inhibitor, aphidicolin, FANCD2 recruits CtIP to transiently stalled, as well as collapsed, replication forks on chromatin. At stalled forks, CtIP cooperates with FANCD2 to promote fork restart and the suppression of new origin firing. Both functions are dependent on BRCA1 that controls the step-wise recruitment of MRE11, FANCD2 and finally CtIP to stalled replication forks, followed by their concerted actions to promote fork recovery.

  11. Quartz tuning fork as a probe of surface oscillations

    NASA Astrophysics Data System (ADS)

    Todoshchenko, I.; Savin, A.; Haataja, M.; Kaikkonen, J.-P.; Hakonen, P. J.

    2017-02-01

    Quartz tuning forks are high-quality mechanical oscillators widely used in low temperature physics as viscometers, thermometers, and pressure sensors. We demonstrate that a fork placed in liquid helium near the surface of solid helium is very sensitive to the oscillations of the solid-liquid interface. We developed a double-resonance read-out technique, which allowed us to detect oscillations of the surface with an accuracy of 1 Å in 10 s. Using this technique, we have investigated crystallization waves in 4He down to 10 mK. In contrast to previous studies of crystallization waves, our measurement scheme has very low dissipation, on the order of 20 pW, which allows us to carry out experiments even at sub-mK temperatures. We propose to use this scheme in the search for crystallization waves in 3He, which exist only at temperatures well below 0.5 mK. The suggested technique can also be used for accurate displacement detection in a large variety of systems.

  12. Environment of deposition of Clear Fork Formation: Yoakum County, Texas

    SciTech Connect

    Moore, B.K.

    1987-05-01

    The Clear Fork Formation is Permian (Leonardian) in age and constitutes a major oil-bearing unit in the Permian basin of west Texas. In Yoakum County, west Texas, the upper Clear Fork carbonates record a subtidal upward-shoaling sequence of deposition. A small bryozoan-algal patch reef is situated within these carbonates near the southern edge of the North Basin platform. The reef is completely dolomitized, but paramorphic replacement has facilitated a study of the paleoecology, lateral variations, and community succession within this buildup. Build-ups of this type are scarcely known in strata of Permian age. The reef was apparently founded on a coquina horizon at the base of the buildup. The reef apparently had a low-relief, dome-shaped morphology. The trapping and binding of sediment by bryozoa appear to have been the main constructional process. A significant role was also played by encrusting forams and the early precipitation of submarine cements, both of which added rigidity to the structure. The reef also contains a low-diversity community of other invertebrates. Algal constituents predominate at the basinward edge of the buildup. The reef was formed entirely subaqueously on a broad, relatively shallow tropical marine carbonate shelf environment. An understanding of the lithofacies distribution and paragenesis within this sequence will provide information on porosity variations and the nature and distribution of permeability barriers. Such information is useful in reservoir modeling studies and for secondary recovery techniques in shelf-edge carbonate reservoirs of this type.

  13. Forked grating coupler optical vortex beam interface for silicon photonics

    NASA Astrophysics Data System (ADS)

    Nadovich, Christopher T.; Kosciolek, Derek J.; Jemison, William D.; Crouse, David T.

    2016-09-01

    The forked grating coupler (FGC) is a novel low-profile device compatible with silicon photonics that is capable of sensitive detection or efficient radiation of Optical Vortex (OV) light beams conveying orbital optical angular momentum (OAM). The FGC device combines the idea of a Bragg coupler with the forked hologram to create an integrated optics device that can selectively and efficiently couple selected optical vortex modes at near-normal incidence into planar confined dielectric waveguide modes of a photonic IC. FGCs retain many of the advantages of Bragg couplers, including convenience of placement and fabrication, reasonable bandwidth, small size, and CMOS process compatibility. In this work, prototype designs of FGC structures for 1550 nm wavelength have been developed for implementation on silicon on insulator (SOI) substrate. Fully vectorial three-dimensional (3D) electromagnetic simulation has allowed performance to be optimized over a range of structural parameters. Results have been evaluated against optical performance metrics including overall efficiency, mode match efficiency, and crosstalk between OV modes. Candidate FGC devices have been fabricated on SOI with e-beam lithography and tested optically. Tolerance to etch depth error has been evaluated.

  14. Replication Fork Stability Confers Chemoresistance in BRCA-deficient Cells

    PubMed Central

    Chaudhuri, Arnab Ray; Callen, Elsa; Ding, Xia; Gogola, Ewa; Duarte, Alexandra A.; Lee, Ji-Eun; Wong, Nancy; Lafarga, Vanessa; Calvo, Jennifer A.; Panzarino, Nicholas J.; John, Sam; Day, Amanda; Crespo, Anna Vidal; Shen, Binghui; Starnes, Linda M.; de Ruiter, Julian R.; Daniel, Jeremy A.; Konstantinopoulos, Panagiotis A.; Cortez, David; Cantor, Sharon B.; Fernandez-Capetillo, Oscar; Ge, Kai; Jonkers, Jos; Rottenberg, Sven; Sharan, Shyam K.; Nussenzweig, André

    2016-01-01

    Brca1- and Brca2-deficient cells have reduced capacity to repair DNA double-strand breaks (DSBs) by homologous recombination (HR) and consequently are hypersensitive to DNA damaging agents, including cisplatin and poly(ADP-ribose) polymerase (PARP) inhibitors. Here we show that loss of the MLL3/4 complex protein, PTIP, protects Brca1/2-deficient cells from DNA damage and rescues the lethality of Brca2-deficient embryonic stem cells. However, PTIP deficiency does not restore HR activity at DSBs. Instead, its absence inhibits the recruitment of the MRE11 nuclease to stalled replication forks, which in turn protects nascent DNA strands from extensive degradation. More generally, acquisition of PARPi and cisplatin resistance is associated with replication fork (RF) protection in Brca2-deficient tumor cells that do not develop Brca2 reversion mutations. Disruption of multiple proteins, including PARP1 and CHD4, leads to the same end point of RF protection, highlighting the complexities by which tumor cells evade chemotherapeutic interventions and acquire drug resistance. PMID:27443740

  15. Landscape controls on nitrogen availability in a western forest watershed

    NASA Astrophysics Data System (ADS)

    Hu, J.; Yano, Y.; Jencso, K. G.

    2015-12-01

    Global increases in anthropogenic nitrogen (N) deposition and declines in winter snowpack are expected to alter the productivity of montane forests in the Western U.S. In this study, we focused on the topographic controls on seasonal N patterns in a mountainous landscape. The experiment was conducted in the North Fork of the Elk Creek (NFEC) watershed within the Lubrecht Experimental Forest, located in western Montana. The NFEC is a managed watershed and was logged approximately 80 years ago; thus, this watershed offers a unique opportunity to examine topographic and biological controls on N dynamics within a relatively even aged forest. In 2014 and 2015, we examined changes in soil N dynamics in four sites that varied in aspect (north and south) and elevation (high and low). Within each site, we also examined micro-topographic controls by sampling soil from both the hollow and the side slope. From pre-snowmelt to the end of the growing season (March - October), we collected snow cores and soil cores within each site to characterize the heterogeneity of soil N. We also installed resin strips to measure the exchangeable N within the upper soils. Across the entire watershed, we found that seasonal NH4+ and NO3- concentrations were highest in April, as well as at the higher elevation sites. This pattern corresponded with higher snowpack and snow water equivalent values and was likely due to higher mineralization rates during the winter. We also found that across all four sites, hollow soils had higher NH4+ and NO3- concentrations relative to soils from the hillslope, and this was largely due to increased organic matter and high soil moisture content found in the hollows. We also found a significant interaction between time of year, site, and micro-topography, suggesting that during the early growing season, NH4+ and NO3- concentrations were largely modulated by snowpack depth, which in turn was controlled by topography. Soil NH4+ and NO3- concentrations for samples

  16. Multi-Scale Soil Moisture Monitoring and Modeling at ARS Watersheds for NASA's Soil Moisture Active Passive (SMAP) Calibration/Validation Mission

    NASA Astrophysics Data System (ADS)

    Coopersmith, E. J.; Cosh, M. H.

    2014-12-01

    NASA's SMAP satellite, launched in November of 2014, produces estimates of average volumetric soil moisture at 3, 9, and 36-kilometer scales. The calibration and validation process of these estimates requires the generation of an identically-scaled soil moisture product from existing in-situ networks. This can be achieved via the integration of NLDAS precipitation data to perform calibration of models at each ­in-situ gauge. In turn, these models and the gauges' volumetric estimations are used to generate soil moisture estimates at a 500m scale throughout a given test watershed by leveraging, at each location, the gauge-calibrated models deemed most appropriate in terms of proximity, calibration efficacy, soil-textural similarity, and topography. Four ARS watersheds, located in Iowa, Oklahoma, Georgia, and Arizona are employed to demonstrate the utility of this approach. The South Fork watershed in Iowa represents the simplest case - the soil textures and topography are relative constants and the variability of soil moisture is simply tied to the spatial variability of precipitation. The Little Washita watershed in Oklahoma adds soil textural variability (but remains topographically simple), while the Little River watershed in Georgia incorporates topographic classification. Finally, the Walnut Gulch watershed in Arizona adds a dense precipitation network to be employed for even finer-scale modeling estimates. Results suggest RMSE values at or below the 4% volumetric standard adopted for the SMAP mission are attainable over the desired spatial scales via this integration of modeling efforts and existing in-situ networks.

  17. STEWARDS: A watershed data system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Comprehensive, long-term data from watersheds across diverse environments are needed for hydrologic and ecosystem analysis and for model development, calibration and validation. To support the Agricultural Research Service's Conservation Effects Assessment Project (CEAP) in assessing impacts of USDA...

  18. Alameda Creeks Healthy Watersheds Project

    EPA Pesticide Factsheets

    Information about the SFBWQP Alameda Creeks Healthy Watersheds Project, part of an EPA competitive grant program to improve SF Bay water quality focused on restoring impaired waters and enhancing aquatic resour

  19. Accomplishments of South Platte Watershed

    EPA Pesticide Factsheets

    Accomplishments of the South Platte Watershed of the Urban Waters Federal Partnership (UWFP) reconnects urban communities with their waterways by improving coordination among federal agencies and collaborating with community-led efforts.

  20. Watershed Simulation of Nutrient Processes

    EPA Science Inventory

    In this presentation, nitrogen processes simulated in watershed models were reviewed and compared. Furthermore, current researches on nitrogen losses from agricultural fields were also reviewed. Finally, applications with those models were reviewed and selected successful and u...

  1. COMPLEMENTARY APPROACHES TO WATERSHED ASSESSMENT

    EPA Science Inventory

    Anthropogenic activities in watersheds affect aquatic organisms, riparian vegetation, and their ability to support avian populations. Our objective was to compare indicators of stream and riparian condition with the composition of breeding bird populations in six Rhode Island s...

  2. Asotin Creek Model Watershed Plan

    SciTech Connect

    Browne, D.; Holzmiller, J.; Koch, F.; Polumsky, S.; Schlee, D.; Thiessen, G.; Johnson, C.

    1995-04-01

    The Asotin Creek Model Watershed Plan is the first to be developed in Washington State which is specifically concerned with habitat protection and restoration for salmon and trout. The plan is consistent with the habitat element of the ``Strategy for Salmon``. Asotin Creek is similar in many ways to other salmon-bearing streams in the Snake River system. Its watershed has been significantly impacted by human activities and catastrophic natural events, such as floods and droughts. It supports only remnant salmon and trout populations compared to earlier years. It will require protection and restoration of its fish habitat and riparian corridor in order to increase its salmonid productivity. The watershed coordinator for the Asotin County Conservation District led a locally based process that combined local concerns and knowledge with technology from several agencies to produce the Asotin Creek Model Watershed Plan.

  3. Working at a Watershed Level

    EPA Pesticide Factsheets

    A short course that provides a basic, but very broad foundation for the use of ecological, social and organizational management principles to guide activities to restore and sustain watershed conditions.

  4. Sediment source identification and load prediction in a mixed-use Piedmont watershed, South Carolina.

    PubMed

    McCarney-Castle, Kerry; Childress, Tristan M; Heaton, Christian R

    2016-10-28

    Many streams in the Piedmont region of the southeastern United States transport a disproportionately large amount of suspended sediment in response to moderately increased streamflows. Transport and deposition of excess sediment affect the stability of the channel and the health of the biological community; therefore, identifying the main source(s) of sediment and assessing the relationships between source, transport, and streamflow are critical to aquatic life and habitat management, dynamic equilibrium preservation, and development of feasible mitigation scenarios. The objectives of this study were to: (1) predict the annual suspended sediment yield and (2) identify significant contributing upland sources of sediment in the Lawsons Fork Creek basin, a 217 km(2) mixed-use watershed in the South Carolina Piedmont. A regularly monitored cross-section located in the downstream reach was equipped with a passive sediment sampler, gage-height recorder, and sediment tiles. Streamflow and sediment concentration were measured over a 24-month period under variable hydrologic regimes. Results indicated that the average annual sediment yield (168 t/km(2)/yr) is significantly higher than yields documented in Piedmont watersheds of comparable size. To identify and prioritize sources of sediment contribution, stable isotopes of nitrogen (δ(15)N) and carbon (δ(13)C) were used as tracers. Source material was compared with suspended sediment near the watershed outlet (target material) and SIAR, a Bayesian Inference model, was used to estimate source apportionment. Results of this source study indicate that approximately 60% of the total sediment load in the water column during high flow events is derived from stream bank erosion. Findings are consistent with observed unstable stream bank conditions in the watershed. This study supports the use of a dual-isotopic fingerprinting approach in tandem with traditional sediment monitoring as a cost-effective method to identify and

  5. Watershed Boundary Dataset for Mississippi

    USGS Publications Warehouse

    Wilson, K. Van; Clair, Michael G.; Turnipseed, D. Phil; Rebich, Richard A.

    2009-01-01

    The U.S. Geological Survey, in cooperation with the Mississippi Department of Environmental Quality, U.S. Department of Agriculture-Natural Resources Conservation Service, Mississippi Department of Transportation, U.S. Department of Agriculture-Forest Service, and the Mississippi Automated Resource Information System developed a 1:24,000-scale Watershed Boundary Dataset for Mississippi including watershed and subwatershed boundaries, codes, names, and areas. The Watershed Boundary Dataset for Mississippi provides a standard geographical framework for water-resources and selected land-resources planning. The original 8-digit subbasins (Hydrologic Unit Codes) were further subdivided into 10-digit watersheds (62.5 to 391 square miles (mi2)) and 12-digit subwatersheds (15.6 to 62.5 mi2) - the exceptions being the Delta part of Mississippi and the Mississippi River inside levees, which were subdivided into 10-digit watersheds only. Also, large water bodies in the Mississippi Sound along the coast were not delineated as small as a typical 12-digit subwatershed. All of the data - including watershed and subwatershed boundaries, subdivision codes and names, and drainage-area data - are stored in a Geographic Information System database, which are available at: http://ms.water.usgs.gov/. This map shows information on drainage and hydrography in the form of U.S. Geological Survey hydrologic unit boundaries for water-resource 2-digit regions, 4-digit subregions, 6-digit basins (formerly called accounting units), 8-digit subbasins (formerly called cataloging units), 10-digit watershed, and 12-digit subwatersheds in Mississippi. A description of the project study area, methods used in the development of watershed and subwatershed boundaries for Mississippi, and results are presented in Wilson and others (2008). The data presented in this map and by Wilson and others (2008) supersede the data presented for Mississippi by Seaber and others (1987) and U.S. Geological Survey (1977).

  6. Walker Branch Watershed Ecosystems Data

    DOE Data Explorer

    Walker Branch Watershed is located on the U. S. Department of Energy's Oak Ridge Reservation near Oak Ridge, in Anderson County, Tennessee. The Walker Branch Watershed Project began in 1967 under sponsorship of the U. S. Atomic Energy Commission (now the U. S. Department of Energy). Initially, the project centered primarily on the geologic and hydrologic processes that control the amounts and chemistry of water moving through the watershed. Past projects have included: • U. S. Department of Energy funded studies of watershed hydrology and forest nutrient dynamics • National Oceanic and Atmospheric Administration funded studies of forest micrometeorology • Studies of atmospheric deposition under the National Atmospheric Deposition Program • The International Biological Program Eastern Deciduous Forest Biome Project • National Science Foundation sponsored studies of trace element cycling and stream nutrient spiraling • Electric Power Research Institute funded studies of the effects of acidic deposition on canopy processes and soil chemistry. These projects have all contributed to a more complete understanding of how forest watersheds function and have provided insights into the solution of energy-related problems associated with air pollution, contaminant transport, and forest nutrient dynamics. This is one of a few sites in the world characterized by long-term, intensive environmental studies. The Walker Branch Watershed website at http://walkerbranch.ornl.gov/ provides maps, photographs, and data on climate, precipitation, atmospheric deposition, stream discharge and runoff, stream chemistry, and vegetation. [Taken from http://walkerbranch.ornl.gov/ABOUTAAA.HTM

  7. Detecting Patterns of Land Use Disturbance at a Watershed Scale: A Study of the Navarro River Watershed using Hyperspectral Data Analysis Techniques

    NASA Astrophysics Data System (ADS)

    Viers, J. H.; Florsheim, J.; Ramirez, C. M.; Quinn, J. F.; Johnson, M. L.; Kozlowicz, B.

    2002-12-01

    Analysis of hyperspectral data is a particularly novel approach to investigation of the relation between anthropogenic and natural disturbances, geomorphic responses, and ecosystem patterns at the watershed scale. During July 2000, hyperspectral imagery was collected for the Navarro basin (820km2) using the Airborne Visible/Infrared Imaging Spectrometer (AVIRIS). This NASA sensor covers the spectral wavelength range from 400nm - 2500nm, with spectral sampling of 10nm in 224 contiguous channels, and spatial resolution of 5m. These data are being analyzed for a variety of applications; however, their use for detecting patterns of disturbance within the watershed is intriguing, especially for the identification and delineation of mass wasting sites that deliver sediment to salmon bearing streams. Mass wasting sites were extracted from AVIRIS imagery using image processing techniques such as Minimum Noise Fraction and Tasseled Cap transformations, image segmentation and masking. These geospatial and spectral data were analyzed for the North Fork of the Navarro River, a sub-basin where spawning habitat for threatened coho salmon is effected by accelerated sediment delivery. Additionally, fieldwork verified the spatial position and dimensions of mass-wasting sites identified from aerial photography. A subset of 1066 identified sites was used for assessing feature extraction error from the AVIRIS imagery; the remaining sites were used for model verification. Augmenting these data within GIS, a multivariate analysis incorporated: proximity to salmon bearing streams; hillslope gradient; landslide position; and timber harvesting to identify patterns of disturbance. Preliminary results indicate that AVIRIS imagery can be segmented to identify exposed soil; furthermore, these identified areas are typically lower elevation, moderately steep hillslopes in constricted river valleys and correspond with mapped delivery sites. Hyperspectral data provide a means for the detection of

  8. Field studies of streamflow generation using natural and injected tracers on Bickford and Walker Branch Watersheds

    SciTech Connect

    Genereux, D.; Hemond, H. . Dept. of Civil Engineering); Mulholland, P. )

    1992-05-01

    Field studies of streamflow generation were undertaken on two forested watersheds, the West Road subcatchment of Bickford Watershed in central Massachusetts and the West Fork of Walker Branch Watershed in eastern Tennessee. A major component of the research was development of a two-stage methodology for the use of naturally-occurring {sup 222}Rn as a tracer. The first of the two stages was solving a mass-balance equation for {sup 222}Rn around a stream reach of interest in order to calculate Rn{sub q}, the {sup 222}Rn content of the lateral inflow to the reach; a conservative tracer (chloride) and a volatile tracer (propane) were injected into the study stream to account for lateral inflow to, and volatilization from, the study reach. The second stage involved quantitative comparison of Rn{sub q} to the measured {sup 222}Rn concentrations of different subsurface waters in order to assess how important these waters were in contributing lateral inflow to the stream reach.

  9. Depositional environments of the Red Fork Sandstone in Custer and Roger Mills Counties, southwestern Oklahoma

    SciTech Connect

    Tolson, P.M. )

    1993-09-01

    The Desmoinesian Red Fork formation is a prolific, overpressured gas-producing sequence of interbedded sandstones and shales. Total thickness ranges from less than 100 ft (northeast) to more than 1100 ft (south). Isopach maps suggest that syndepositional faulting controlled major depositional trends. The lower Red Fork, whose base is defined by a persistent, hot, black shale (sequence boundary ), is mainly deep-marine shale and siltstone. Two major shallowing-upward deltaic sequences separated by a marine transgression are evident in the middle (50-400-ft thick) and upper (30-250-ft thick) Red Fork. The middle Red Fork is marine dominated and was deposited into a relatively deep basin on a steep, unstable delta-front slope. In contrast, the upper Red Fork deltaic sequence is more fluvial dominated and was deposited in shallower water. The upper Red Fork is overlain by the Pink lime interval which appears to be shallow-marine/lagoonal black shale. The Pink lime contains fish scales, coffee-ground to branch-size lignitic plant debris, and brackish to shallow-marine ostracodes, linguloid brachiopods, Tasmanites algae, and gastropods. Most of the Red Fork has an easterly, possibly Ouachita Mountain area source. The prolific Southwest Leedey field has a different mineral assemblage and diagenetic sequence and may have a northern source.

  10. Geomorphic Characterization of the Middle Fork Saline River: Garland, Perry, and Saline Counties, Arkansas

    USGS Publications Warehouse

    Pugh, Aaron L.; Garday, Thomas J.; Redman, Ronald

    2008-01-01

    This report was prepared to help address concerns raised by local residents, State, and Federal agencies about the current geomorphic conditions of the Middle Fork Saline River. Over the past 30 years the Middle Fork Saline River Basin has experienced a marked increase in urbanization. The report summarizes the Middle Fork?s current (2003) channel characteristics at nine stream reaches in the upper 91 square miles of the basin. Assessments at each study reach included comparing measured stream geometry dimensions (cross-sectional area, top width, and mean depth) at bankfull stage to regional hydraulic geometry curves for the Ouachita Mountains Physiographic Province of Arkansas and Oklahoma, evaluations of streambed materials and sinuosity, and classification of individual stream reach types. When compared to the Ouachita Mountains? regional hydraulic geometry curves for natural, stable, stream reaches, five of the nine study reaches had slightly smaller crosssectional areas, longer top widths, and shallower depths. Streambed material analysis indicates that the Middle Fork is a bedrock influenced, gravel dominated stream with lesser amounts of sand and cobbles. Slight increases in sinuosity from 1992 to 2002 at seven of the nine study reaches indicate a slight decrease in stream channel slope. Analyses of the Middle Fork?s hydraulic geometry and sinuosity indicate that the Middle Fork is currently overly wide and shallow, but is slowly adjusting towards a deeper, narrower hydraulic geometry. Using the Rosgen system of channel classification, the two upstream study reaches classified as B4c/1 stream types; which were moderately entrenched, riffle dominated channels, with infrequently spaced pools. The downstream seven study reaches classified as C4/1 stream types; which were slightly entrenched, meandering, gravel-dominated, riffle/ pool channels with well developed flood plains. Analyses of stream reach types suggest that the downstream reaches of the Middle Fork

  11. Effects of Transposable Elements on the Expression of the Forked Gene of Drosophila Melanogaster

    PubMed Central

    Hoover, K. K.; Chien, A. J.; Corces, V. G.

    1993-01-01

    The products of the forked gene are involved in the formation and/or maintenance of a temporary fibrillar structure within the developing bristle rudiment of Drosophila melanogaster. Mutations in the forked locus alter this structure and result in aberrant development of macrochaetae, microchaetae and trichomes. The locus has been characterized at the molecular level by walking, mutant characterization and transcript analysis. Expression of the six forked transcripts is temporally restricted to midlate pupal development. At this time, RNAs of 6.4, 5.6, 5.4, 2.5, 1.9 and 1.1 kilobases (kb) are detected by Northern analysis. The coding region of these RNAs has been found to be within a 21-kb stretch of genomic DNA. The amino terminus of the proteins encoded by the 5.4- and 5.6-kb forked transcripts contain tandem copies of ankyrin-like repeats that may play an important role in the function of forked-encoded products. The profile of forked RNA expression is altered in seven spontaneous mutations characterized during this study. Three forked mutations induced by the insertion of the gypsy retrotransposon contain a copy of this element inserted into an intron of the gene. In these mutants, the 5.6-, 5.4- and 2.5-kb forked mRNAs are truncated via recognition of the polyadenylation site in the 5' long terminal repeat of the gypsy retrotransposon. These results help explain the role of the forked gene in fly development and further our understanding of the role of transposable elements in mutagenesis. PMID:8244011

  12. Supplement Analysis for the Watershed Management Program EIS (DOE/EIS-0265/SA-100) - Oregon Fish Screening Project, Screen Replacements 2003

    SciTech Connect

    Stewart, Shannon C.

    2002-11-25

    BPA is proposing to fund a project that will provide immediate and long-term protection for anadromous and resident fish through the replacement of out-of-criteria screening devices on private irrigation diversions and dam structures. The goal of this project is to replace approximately 20 screening devices during the 2003 work period. These screening projects will take place in the mainstem, middle fork, and south fork of the John Day River and, if the need arises, in the Umatilla/Walla Walla and Grande Ronde/Imnaha subbasins. Projects are prioritized using the Priority Screening Criteria point system adopted by ODFW from the Oregon Watershed Enhancement Board. Factors considered in prioritization include: fish species status, fish numbers, diversion size, diversion flow relative to stream, fish migration, habitat quality, basin priority, and diversions screened within the stream system.

  13. Finite Element Analysis of Electrically Excited Quartz Tuning Fork Devices

    PubMed Central

    Oria, Roger; Otero, Jorge; González, Laura; Botaya, Luis; Carmona, Manuel; Puig-Vidal, Manel

    2013-01-01

    Quartz Tuning Fork (QTF)-based Scanning Probe Microscopy (SPM) is an important field of research. A suitable model for the QTF is important to obtain quantitative measurements with these devices. Analytical models have the limitation of being based on the double cantilever configuration. In this paper, we present an electromechanical finite element model of the QTF electrically excited with two free prongs. The model goes beyond the state-of-the-art of numerical simulations currently found in the literature for this QTF configuration. We present the first numerical analysis of both the electrical and mechanical behavior of QTF devices. Experimental measurements obtained with 10 units of the same model of QTF validate the finite element model with a good agreement. PMID:23722828

  14. Quartz-enhanced photoacoustic spectroscopy exploiting tuning fork overtone modes

    NASA Astrophysics Data System (ADS)

    Sampaolo, A.; Patimisco, P.; Dong, L.; Geras, A.; Scamarcio, G.; Starecki, T.; Tittel, F. K.; Spagnolo, V.

    2015-12-01

    We report on a quartz-enhanced photoacoustic sensor (QEPAS) based on a custom-made quartz tuning fork (QTF) to operate in both the fundamental and the first overtone vibrational mode resonances. The QTF fundamental mode resonance falls at ˜3 kHz and the first overtone at ˜18 kHz. Electrical tests showed that the first overtone provides a higher quality factor and increased piezoelectric current peak values, with respect to the fundamental flexural mode. To evaluate the QTF acousto-electric energy conversion efficiency, we operated the QEPAS in the near-IR and selected water vapor as the target gas. The first overtone resonance provides a QEPAS signal-to-noise ratio ˜5 times greater with respect to that measured for the fundamental mode. These results open the way to employing QTF overtone vibrational modes for QEPAS based trace gas sensing.

  15. Geochemical map of the North Fork John Day River Roadless Area, Grant County, Oregon

    USGS Publications Warehouse

    Evans, James G.

    1986-01-01

    The North Fork John Day River Roadless Area comprised 21,210 acres in the Umatilla and Wallowa-Whitman National Forests, Grant County, Oregon, about 30 miles northwest of Baker, Oregon. The irregularly shaped area extends for about 1 mile on both sides of a 25-mile segment of the North Fork John Day River from Big Creek on the west to North Fork John Day Campground on the east. Most of the roadless area is in the northern half of the Desolation Butte 15-minute quadrangle. The eastern end of the area is in parts of the Granite and Trout Meadows 7½-minute quadrangles.

  16. Managing landscape disturbances to increase watershed infiltration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agricultural land undergoing conversion to conventional urban development can drastically increase runoff and degrade water quality. A study of landscape management for improving watershed infiltration was conducted using readily available runoff data from experimental watersheds. This article focus...

  17. GROUND WATER AND WATERSHEDS AND ENVIRONMENTAL PROTECTION

    EPA Science Inventory

    Effective watershed management has the potential to achieve both drinking water and ecological protection goals. However, it is important that the watershed perspective be three- dimensional and include the hidden subsurface. The subsurface catchment, or groundwatershed, is geohy...

  18. ASSESSMENT AND MANAGEMENT OF WATERSHED MICROBIAL CONTAMINANTS

    EPA Science Inventory

    Numerous sources of infectious disease causing microorganisms exist in watersheds and can impact recreational and drinking water quality. Organisms of concern include bacteria, viruses, and parasites. The watershed manager is challenged to limit human contact with pathogens, limi...

  19. Watershed Management in the United States

    EPA Science Inventory

    A watershed approach provides an effective framework for dealing with water resources challenges. Watersheds provide drinking water, recreation, and ecological habitat, as well as a place for waste disposal, a source of industrial cooling water, and navigable inland water transpo...

  20. Iowa flood studies (IFloodS) in the South Fork experimental watershed: soil moisture and precipitation monitoring

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Soil moisture estimates are valuable for hydrologic modeling and agricultural decision support. These estimates are typically produced via a combination of sparse ¬in situ networks and remotely-sensed products or where sensory grids and quality satellite estimates are unavailable, through derived h...

  1. Soil moisture and precipitation monitoring in the South Fork experimental watershed during the Iowa flood studies (IFloodS)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Soil moisture estimates are valuable for hydrologic modeling and agricultural decision support. These estimates are typically produced via a combination of sparse in situ networks and remotely-sensed products or where sensory grids and quality satellite estimates are unavailable, through derived hy...

  2. ASSESSING NON-POINT SOURCES OF NITROGEN TO SMALL STREAMS IN THE SOUTH FORK BROAD RIVER WATERSHED (GEORGIA, USA)

    EPA Science Inventory

    The National Land Cover Data (NLCD) is a land cover classification derived from Landsat Thematic Mapper satellite data collected in the early to mid-1990s. In this work, land use coverages calculated from the NLCD database are used to assess the impact of non-point sources on the...

  3. Antibiotic resistance and community analysis of surface and subsurface drainage waters in the South Fork Iowa River watershed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Midwest is a center for swine production leading to application of swine manure onto lands that have artificial subsurface drainage. Previous reports have indicated elevated levels of antibiotic resistance genes (ARGs) in surface water and groundwater around confined animal feeding operations w...

  4. Developing Water Quality Management Strategies At Different Watershed Scales Using System Dynamics Simulation

    NASA Astrophysics Data System (ADS)

    Tangirala, A.; Teegavarapu, R.; Ormsbee, L.

    2003-12-01

    System Dynamics simulation is used for developing models to understand the fate and transport of pathogens at different watershed scales. The models are developed using an object-oriented modeling environment to simulate and analyze water quality management strategies for pathogen impaired streams. The main objective of this work is to evaluate the relative contributions of pathogen loads from each watershed to facilitate the budget allocations that have to be made in each watershed and also to understand the fate and transport of pathogens in impaired streams of each watershed. The models can be used to generate several management scenarios and evaluate several adaptive management alternatives that are designed to reduce further impairment. An object-oriented simulation environment, conceived on the principles of system dynamics (SD) is used for the development of the model. Two important generic building blocks, stocks and flows that are part of the simulation environment are used to model various elements that govern the water bodies and pollutant loadings. The modeling process consists of developing, stock-flow diagrams and finally carrying out computer simulations using difference equations to integrate stocks and flows. The model structure and behavior of the system is validated using dimensionality, replication and sensitivity tests; and calibration along with validation is carried out using appropriate model performance measures. Case study application of this concept is illustrated by developing simulation models for pathogen impaired streams in the three watersheds, North Fork, Upper Cumberland and Big Sandy in southeastern region of Kentucky, USA. Straight pipes and failing septic systems have been identified to be the major sources of pathogen impairment in these watersheds and therefore are key factors influencing management alternatives. Preliminary results suggest that the fate and transport process of pathogens in a river system and at a larger

  5. Cancer therapy and replication stress: forks on the road to perdition.

    PubMed

    Kotsantis, Panagiotis; Jones, Rebecca M; Higgs, Martin R; Petermann, Eva

    2015-01-01

    Deregulated DNA replication occurs in cancer where it contributes to genomic instability. This process is a target of cytotoxic therapies. Chemotherapies exploit high DNA replication in cancer cells by modifying the DNA template or by inhibiting vital enzymatic activities that lead to slowing or stalling replication fork progression. Stalled replication forks can be converted into toxic DNA double-strand breaks resulting in cell death, i.e., replication stress. While likely crucial for many cancer treatments, replication stress is poorly understood due to its complexity. While we still know relatively little about the role of replication stress in cancer therapy, technical advances in recent years have shed new light on the effect that cancer therapeutics have on replication forks and the molecular mechanisms that lead from obstructed fork progression to cell death. This chapter will give an overview of our current understanding of replication stress in the context of cancer therapy.

  6. The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability

    PubMed Central

    Steinacher, Roland; Osman, Fekret; Dalgaard, Jacob Z.; Lorenz, Alexander; Whitby, Matthew C.

    2012-01-01

    Bidirectionally moving DNA replication forks merge at termination sites composed of accidental or programmed DNA–protein barriers. If merging fails, then regions of unreplicated DNA can result in the breakage of DNA during mitosis, which in turn can give rise to genome instability. Despite its importance, little is known about the mechanisms that promote the final stages of fork merging in eukaryotes. Here we show that the Pif1 family DNA helicase Pfh1 plays a dual role in promoting replication fork termination. First, it facilitates replication past DNA–protein barriers, and second, it promotes the merging of replication forks. A failure of these processes in Pfh1-deficient cells results in aberrant chromosome segregation and heightened genome instability. PMID:22426535

  7. Grays River Watershed Geomorphic Analysis

    SciTech Connect

    Geist, David R

    2005-04-30

    This investigation, completed for the Pacific Northwest National Laboratory (PNNL), is part of the Grays River Watershed and Biological Assessment commissioned by Bonneville Power Administration under project number 2003-013-00 to assess impacts on salmon habitat in the upper Grays River watershed and present recommendations for habitat improvement. This report presents the findings of the geomorphic assessment and is intended to support the overall PNNL project by evaluating the following: The effects of historical and current land use practices on erosion and sedimentation within the channel network The ways in which these effects have influenced the sediment budget of the upper watershed The resulting responses in the main stem Grays River upstream of State Highway 4 The past and future implications for salmon habitat.

  8. Probing Liquid ^4He with Quartz Tuning Forks Using a Novel Multifrequency Lock-in Technique

    NASA Astrophysics Data System (ADS)

    Bradley, D. I.; Haley, R. P.; Kafanov, S.; Noble, M. T.; Pickett, G. R.; Tsepelin, V.; Vonka, J.; Wilcox, T.

    2016-09-01

    We report on a novel technique to measure quartz tuning forks, and possibly other vibrating objects, in a quantum fluid using a multifrequency lock-in amplifier. The multifrequency technique allows to measure the resonance curve of a vibrating object much faster than a conventional single frequency lock-in amplifier technique. Forks with resonance frequencies of 12 kHz and 16 kHz were excited and measured electro-mechanically either at a single frequency or at up to 40 different frequencies simultaneously around the same mechanical mode. The response of each fork was identical for both methods and validates the use of the multifrequency lock-in technique to probe properties of liquid helium at low fork velocities. Using both methods we measured the resonance frequency and drag of two 25-μ m-wide quartz tuning forks immersed in liquid ^4He in the temperature range from 4.2 K to 1.5 K at saturated vapour pressure. The damping and shift of resonance frequency experienced by both tuning forks at low velocities are well described by hydrodynamic contributions in the framework of the two-fluid model. The sensitivity of the 25-μ m-wide tuning forks is larger compared to similar 75-μ m-wide forks and in combination with the faster multifrequency lock-in technique could be used to improve thermometry in liquid ^4He. The multifrequency technique could also be used for studies of the onset of non-linear phenomena such as quantum turbulence and cavitation in superfluids.

  9. Environmental Assessment: Demolish CASS Switch Stations Buildings 644, 645, 646 at Grand Forks Air Force Base

    DTIC Science & Technology

    2006-06-01

    Winters are long and severe with almost continuous snow cover. The spring and fall seasons are generally short transition periods. The average...during the late fall, winter, and spring , and from the southeast during the summer. Grand Forks County is included in the ND Air Quality Control...approximately 10 miles northeast of Grand Forks AFB, the mean discharge of the Turtle River is 50.3 feet cubed per second (ft3/s). Peak flows result from

  10. Environmental Assessment: Construct Airfield Lighting Vault and Demolish Building 531 at Grand Forks Air Force Base

    DTIC Science & Technology

    2007-08-01

    frequent thunderstorms. Winters are long and severe with almost continuous snow cover. The spring and fall seasons are generally short transition periods...from the northwest during the late fall, winter, and spring , and from the southeast during the summer. Grand Forks County is included in the ND Air...Manvel, ND, approximately 10 miles northeast of Grand Forks AFB, the mean discharge of the Turtle River is 50.3 feet cubed per second (ft3/s). Peak

  11. Environmental Assessment: Demolish Buildings 212, 218, 819, 820 at Grand Forks Air Force Base

    DTIC Science & Technology

    2007-01-31

    Winters are long and severe with almost continuous snow cover. The spring and fall seasons are generally short transition periods. The average annual...during the late fall, winter, and spring , and from the southeast during the summer. Grand Forks County is included in the ND Air Quality Control...approximately 10 miles northeast of Grand Forks AFB, the mean discharge of the Turtle River is 50.3 feet cubed per second (ft3/s). Peak flows result from

  12. The Sound Field around a Tuning Fork and the Role of a Resonance Box

    ERIC Educational Resources Information Center

    Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

    2015-01-01

    Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: (1) Why is a bare tuning fork such a weak emitter of sound? (2) What is the role of the…

  13. Watershed Education for Broadcast Meteorologists

    NASA Astrophysics Data System (ADS)

    Lamos, J. P.; Sliter, D.; Espinoza, S.; Spangler, T. C.

    2006-12-01

    The National Environmental Education and Training Organization (NEETF) published a report in 2005 that summarized the findings of ten years of NEETF and Roper Research. The report stated, "Our years of data from Roper surveys show a persistent pattern of environmental ignorance even among the most educated and influential members of society." Market research has also shown that 80% of television viewers list the weather as the primary reason for watching the local news. Broadcast meteorologists, with a broader understanding of environmental and related sciences have an opportunity to use their weathercasts to inform the public about the environment and the factors that influence environmental health. As "station scientists," broadcast meteorologists can use the weather, and people's connection to it, to broaden their understanding of the environment they live in. Weather and watershed conditions associated with flooding and drought have major human and environmental impacts. Increasing the awareness of the general public about basic aspects of the hydrologic landscape can be an important part of mitigating the adverse effects of too much or too little precipitation, and of protecting the environment as well. The concept of a watershed as a person's natural neighborhood is a very important one for understanding hydrologic and environmental issues. Everyone lives in a watershed, and the health of a watershed is the result of the interplay between weather and human activity. This paper describes an online course to give broadcast meteorologists a basic understanding of watersheds and how watersheds are impacted by weather. It discusses how to convey watershed science to a media- savvy audience as well as how to model the communication of watershed and hydrologic concepts to the public. The course uses a narrative, story-like style to present its content. It is organized into six short units of instruction, each approximately 20 minutes in duration. Each unit is

  14. Groundwater and surface-water interaction within the upper Smith River Watershed, Montana 2006-2010

    USGS Publications Warehouse

    Caldwell, Rodney R.; Eddy-Miller, Cheryl A.

    2013-01-01

    The 125-mile long Smith River, a tributary of the Missouri River, is highly valued as an agricultural resource and for its many recreational uses. During a drought starting in about 1999, streamflow was insufficient to meet all of the irrigation demands, much less maintain streamflow needed for boating and viable fish habitat. In 2006, the U.S. Geological Survey, in cooperation with the Meagher County Conservation District, initiated a multi-year hydrologic investigation of the Smith River watershed. This investigation was designed to increase understanding of the water resources of the upper Smith River watershed and develop a detailed description of groundwater and surface-water interactions. A combination of methods, including miscellaneous and continuous groundwater-level, stream-stage, water-temperature, and streamflow monitoring was used to assess the hydrologic system and the spatial and temporal variability of groundwater and surface-water interactions. Collectively, data are in agreement and show: (1) the hydraulic connectedness of groundwater and surface water, (2) the presence of both losing and gaining stream reaches, (3) dynamic changes in direction and magnitude of water flow between the stream and groundwater with time, (4) the effects of local flood irrigation on groundwater levels and gradients in the watershed, and (5) evidence and timing of irrigation return flows to area streams. Groundwater flow within the alluvium and older (Tertiary) basin-fill sediments generally followed land-surface topography from the uplands to the axis of alluvial valleys of the Smith River and its tributaries. Groundwater levels were typically highest in the monitoring wells located within and adjacent to streams in late spring or early summer, likely affected by recharge from snowmelt and local precipitation, leakage from losing streams and canals, and recharge from local flood irrigation. The effects of flood irrigation resulted in increased hydraulic gradients

  15. DNA2 drives processing and restart of reversed replication forks in human cells

    PubMed Central

    Thangavel, Saravanabhavan; Berti, Matteo; Levikova, Maryna; Pinto, Cosimo; Gomathinayagam, Shivasankari; Vujanovic, Marko; Zellweger, Ralph; Moore, Hayley; Lee, Eu Han; Hendrickson, Eric A.; Cejka, Petr; Stewart, Sheila; Lopes, Massimo

    2015-01-01

    Accurate processing of stalled or damaged DNA replication forks is paramount to genomic integrity and recent work points to replication fork reversal and restart as a central mechanism to ensuring high-fidelity DNA replication. Here, we identify a novel DNA2- and WRN-dependent mechanism of reversed replication fork processing and restart after prolonged genotoxic stress. The human DNA2 nuclease and WRN ATPase activities functionally interact to degrade reversed replication forks with a 5′-to-3′ polarity and promote replication restart, thus preventing aberrant processing of unresolved replication intermediates. Unexpectedly, EXO1, MRE11, and CtIP are not involved in the same mechanism of reversed fork processing, whereas human RECQ1 limits DNA2 activity by preventing extensive nascent strand degradation. RAD51 depletion antagonizes this mechanism, presumably by preventing reversed fork formation. These studies define a new mechanism for maintaining genome integrity tightly controlled by specific nucleolytic activities and central homologous recombination factors. PMID:25733713

  16. Multiple mechanisms contribute to double-strand break repair at rereplication forks in Drosophila follicle cells

    PubMed Central

    Alexander, Jessica L.; Beagan, Kelly; Orr-Weaver, Terry L.; McVey, Mitch

    2016-01-01

    Rereplication generates double-strand breaks (DSBs) at sites of fork collisions and causes genomic damage, including repeat instability and chromosomal aberrations. However, the primary mechanism used to repair rereplication DSBs varies across different experimental systems. In Drosophila follicle cells, developmentally regulated rereplication is used to amplify six genomic regions, two of which contain genes encoding eggshell proteins. We have exploited this system to test the roles of several DSB repair pathways during rereplication, using fork progression as a readout for DSB repair efficiency. Here we show that a null mutation in the microhomology-mediated end-joining (MMEJ) component, polymerase θ/mutagen-sensitive 308 (mus308), exhibits a sporadic thin eggshell phenotype and reduced chorion gene expression. Unlike other thin eggshell mutants, mus308 displays normal origin firing but reduced fork progression at two regions of rereplication. We also find that MMEJ compensates for loss of nonhomologous end joining to repair rereplication DSBs in a site-specific manner. Conversely, we show that fork progression is enhanced in the absence of both Drosophila Rad51 homologs, spindle-A and spindle-B, revealing homologous recombination is active and actually impairs fork movement during follicle cell rereplication. These results demonstrate that several DSB repair pathways are used during rereplication in the follicle cells and their contribution to productive fork progression is influenced by genomic position and repair pathway competition. Furthermore, our findings illustrate that specific rereplication DSB repair pathways can have major effects on cellular physiology, dependent upon genomic context. PMID:27849606

  17. Geology of the Cane Branch and Helton Branch watershed areas, McCreary County, Kentucky

    USGS Publications Warehouse

    Lyons, Erwin J.

    1957-01-01

    Cane Branch and Helton Branch in McCreary County, Kentucky, are about 1.4 miles apart (fig. 1). Can Branch, which is about 2.1 miles long, emptied into Hughes Fork of Beaver Creek. Its watershed area of about 1.5 square miles lies largely in the Wiborf 7 1/2-minute quadrangle (SW/4 Cumberland Falls 15-minute quadrangle), but the downstream part of the area extends northward into the Hail 7 1/2-minute quadrangle (NW/4 Cumberland Falls 15-minute quadrangle). Helton Branch, which is about 1.1 miles long, has two tributaries and empties into Little Hurricane Fork of Beaver Creek. It drains an area of about 0.8 square mile of while about 0.5 square mile is in the Hail quadrangle and the remainder in the Wilborg quadrangle. The total relief in the Can Branch area is about 500 feet and in the Helton Branch area about 400 feet. Narrow, steep-sided to canyon-like valley and winding ridges, typical of the Pottsville escarpment region, are characteristic of both areas. Thick woods and dense undergrowth cover much of the two areas. Field mapping was done on U.S. Geological Survey 7 1/2-minute maps having a scale of 1:24,000 and a contour interval of 20 feet. Elevations of lithologic contacts were determined with a barometer and a hand level. Aerial photographs were used principally to trace the cliffs formed by sandstone and conglomerate ledges. Exposures, except for those of the cliff- and ledge-forming sandstone and conglomerates, are not abundant. The most complete stratigraphic sections (secs. 3 and 4, fig. 2) in the two areas are exposed in cuts of newly completed Forest Service roads, but the rick in the upper parts of the exposures is weathered. To supplement these sections, additional sections were measured in cuts along the railroad and main highways in nor near the watersheds.

  18. Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork.

    PubMed

    Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

    2015-06-01

    A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr(1-x)Ti(x))O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever.

  19. MARYLAND AGRICULTURE AND YOUR WATERSHED

    EPA Science Inventory



    Using primarily 1995 State of Maryland agricultural statistics data, a new methodology was demonstrated with which State natural resource managers can analyze the areal extent of agricultural lands and production data on a watershed basis. The report organized major crop ...

  20. WATERSHED-BASED SURVEY DESIGNS

    EPA Science Inventory

    Water-based sampling design and assessment tools help serve the multiple goals for water quality monitoring required under the Clean Water Act, including assessment of regional conditions to meet Section 305(b), identification if impaired water bodies or watersheds to meet Sectio...

  1. MANAGING URBAN WATERSHED PATHOGEN CONTAMINATION

    EPA Science Inventory

    This presentation is a summary of the EPA National Risk Management Research Laboratory (NRMRL) publication entitled Managing Urban Watershed Pathogen Contamination, EPA/600/R-03/111 (September 2003). It is available on the internet at http://www.epa.gov/ednnrmrl/repository/water...

  2. Discover a Watershed: The Everglades.

    ERIC Educational Resources Information Center

    Robinson, George B.; And Others

    This publication is designed for both classroom teachers and nonformal educators of young people in grades 6 through 12. It can provide a 6- to 8-week course of study on the watershed with students participating in activities as they are ordered in the guide, or activities may be used in any order with educators selecting those appropriate for the…

  3. Combined use of PAH levels and EROD activities in the determination of PAH pollution in flathead mullet (Mugil cephalus) caught from the West Black Sea coast of Turkey.

    PubMed

    Bozcaarmutlu, Azra; Sapmaz, Canan; Kaleli, Gizem; Turna, Sema; Yenisoy-Karakaş, Serpil

    2015-02-01

    The aim of this study was to determine the extent of polycyclic aromatic hydrocarbon (PAH) pollution by measuring PAH levels and 7-ethoxyresorufin-O-deethylase (EROD) activities in flathead mullet (Mugil cephalus) samples caught from the West Black Sea coast of Turkey. The fish samples were caught in August 2008-2011. The levels of 13 PAHs were measured by high-performance liquid chromatography (HPLC) in the liver of fish. Most of the measured PAHs had three rings (low molecular weight). The frequencies of detection of PAHs were higher in fish samples caught from Zonguldak Harbour and Gülüç Stream Mouth than those from Sakarya River Mouth, Amasra and Kefken. EROD activities and cytochrome P4501A (CYP1A) protein level were also measured in the fish liver microsomes. Highly elevated EROD activities and CYP1A levels were measured in the mullet samples caught from Zonguldak Harbour and Gülüç Stream than those from Amasra and Kefken. The detection of PAHs in the liver of fish samples shows recent exposure to PAHs. The chemical analyses of PAHs and EROD activity results together reflected the extent of PAH pollution in the livers of fish caught from the West Black Sea coast of Turkey. The results indicate that Zonguldak Harbour is the most polluted site in the West Black Sea coast of Turkey.

  4. Diel changes in stable carbon isotope ratios and trace element concentrations in the Clark Fork River, MT.

    NASA Astrophysics Data System (ADS)

    Parker, S.; Gammons, C.; Degrandpre, M.

    2004-12-01

    A diel (24-hr) water sampling was conducted on the Clark Fork River near Deer Lodge, Montana from 31-July to 1-August of 2003. The Clark Fork River is located in southwestern Montana and its upper reaches have been heavily affected by historic mining and smelting activities in the Butte and Anaconda areas. River floodplains and sediment beds contain significant quantities of metals (iron, aluminum, copper, zinc, lead, cadmium.) and arsenic. Two sites about 1.2 km apart, were sampled with a mean transit time for the water of 2.5 hours between the sites. The river in the study reach is characterized by oxic conditions, moderate alkalinity, moderate biological productivity and a pH range of about 8.0 to 8.5 during the summer low water months. During the mid-summer months, water in the Clark Fork River and its tributary streams is diverted for irrigation. Some of this water returns to the main stem in a chemically modified form as surface or groundwater return flow. This greatly complicates the hydrogeology and nutrient balance of the watershed. The two samplings sites used in this study make it possible to analyze the changes in chemical and physical properties of the water as it travels between the sites. Results reported here show that there is a large degree of temporal and spatial variability across the study area. In situ instruments were deployed and hourly water samples were collected for analysis from both sites. Diel concentration cycles are documented for dissolved forms of manganese, zinc and nitrate. Particulate forms of zinc, aluminum, copper, iron and manganese are shown to undergo twenty-four hour concentration changes. Total suspended solids (TSS) also show a diel change with the mass of TSS increasing at night. Dissolved arsenic is shown to undergo a diurnal concentration cycle at both sampling sites that was out of phase by the average 2.5 hour transit time between the two sites. This arsenic fluctuation may be a pulse of As that is being advected

  5. Mcm10: A Dynamic Scaffold at Eukaryotic Replication Forks

    PubMed Central

    Baxley, Ryan M.; Bielinsky, Anja-Katrin

    2017-01-01

    To complete the duplication of large genomes efficiently, mechanisms have evolved that coordinate DNA unwinding with DNA synthesis and provide quality control measures prior to cell division. Minichromosome maintenance protein 10 (Mcm10) is a conserved component of the eukaryotic replisome that contributes to this process in multiple ways. Mcm10 promotes the initiation of DNA replication through direct interactions with the cell division cycle 45 (Cdc45)-minichromosome maintenance complex proteins 2-7 (Mcm2-7)-go-ichi-ni-san GINS complex proteins, as well as single- and double-stranded DNA. After origin firing, Mcm10 controls replication fork stability to support elongation, primarily facilitating Okazaki fragment synthesis through recruitment of DNA polymerase-α and proliferating cell nuclear antigen. Based on its multivalent properties, Mcm10 serves as an essential scaffold to promote DNA replication and guard against replication stress. Under pathological conditions, Mcm10 is often dysregulated. Genetic amplification and/or overexpression of MCM10 are common in cancer, and can serve as a strong prognostic marker of poor survival. These findings are compatible with a heightened requirement for Mcm10 in transformed cells to overcome limitations for DNA replication dictated by altered cell cycle control. In this review, we highlight advances in our understanding of when, where and how Mcm10 functions within the replisome to protect against barriers that cause incomplete replication. PMID:28218679

  6. Bioavailability of mercury in East Fork Poplar Creek soils

    SciTech Connect

    Barnett, M.O.; Turner, R.R.

    1995-05-01

    The initial risk assessment for the East Fork Poplar Creek (EFPC) floodplain in Oak Ridge, Tennessee, a superfund site heavily contaminated with mercury, was based upon a reference dose for mercuric chloride, a soluble mercury compound not expected to be present in the floodplain, which is frequently saturated with water. Previous investigations had suggested mercury in the EFPC floodplain was less soluble and therefore less bioavailable than mercuric chloride, possibly making the results of the risk assessment unduly conservative. A bioavailability study, designed to measure the amount of mercury available for absorption in a child`s digestive tract, the most critical risk endpoint and pathway, was performed on twenty soils from the EFPC floodplain. The average percentage of mercury released during the study for the twenty soils was 5.3%, compared to 100% of the compound mercuric chloride subjected to the same conditions. Alteration of the procedure to test additional conditions possible during soil digestion did not appreciably alter the results. Therefore, use of a reference dose for mercuric chloride in the EFPC risk assessment without inclusion of a corresponding bioavailability factor may be unduly conservative.

  7. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    SciTech Connect

    ADAMS, S.M.; ASHWOOD, T.L.; BEATY, T.W.; BRANDT, C.C.

    1997-10-24

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y- 12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  8. Biological monitoring program for East Fork Poplar Creek

    SciTech Connect

    Adams, S.M.; Ashwood, T.L.; Beaty, T.W.; Brandt, C.C.; Christensen, S.W.; Cicerone, D.S.; Greeley, M.S. Jr.; Hill, W.R.; Kszos, L.S.

    1997-04-18

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  9. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    SciTech Connect

    ADAMS, S.M.; BEATY, T.W.; BRANDT, C.C.; CHRISTENSEN, S.W.; CICERONE, D.S.

    1998-09-09

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  10. CDK1 phosphorylates WRN at collapsed replication forks

    PubMed Central

    Palermo, Valentina; Rinalducci, Sara; Sanchez, Massimo; Grillini, Francesca; Sommers, Joshua A.; Brosh, Robert M.; Zolla, Lello; Franchitto, Annapaola; Pichierri, Pietro

    2016-01-01

    Regulation of end-processing is critical for accurate repair and to switch between homologous recombination (HR) and non-homologous end joining (NHEJ). End resection is a two-stage process but very little is known about regulation of the long-range resection, especially in humans. WRN participates in one of the two alternative long-range resection pathways mediated by DNA2 or EXO1. Here we demonstrate that phosphorylation of WRN by CDK1 is essential to perform DNA2-dependent end resection at replication-related DSBs, promoting HR, replication recovery and chromosome stability. Mechanistically, S1133 phosphorylation of WRN is dispensable for relocalization in foci but is involved in the interaction with the MRE11 complex. Loss of WRN phosphorylation negatively affects MRE11 foci formation and acts in a dominant negative manner to prevent long-range resection altogether, thereby licensing NHEJ at collapsed forks. Collectively, we unveil a CDK1-dependent regulation of the WRN-DNA2-mediated resection and identify an undescribed function of WRN as a DSB repair pathway switch. PMID:27634057

  11. Spatial variations in the fate and transport of metals in a mining-influenced stream, North Fork Clear Creek, Colorado.

    PubMed

    Butler, Barbara A; Ranville, James F; Ross, Philippe E

    2009-12-01

    North Fork Clear Creek (NFCC) receives acid-mine drainage (AMD) from multiple abandoned mines in the Clear Creek Watershed. Point sources of AMD originate in the Black Hawk/Central City region of the stream. Water chemistry also is influenced by several non-point sources of AMD, and a wastewater treatment plant (WWTP). In-stream conditions immediately downstream from point-source inputs result in a visual and rapid precipitation of hydrous iron oxides (HFO). Hydrous manganese oxides (HMO) are seen to coat rocks further downstream during some seasons. Synoptic spatial sampling was used to assess the fate and transport of Cu, Fe, Mn, and Zn during different years and hydrological seasons. Visual-MINTEQ was used to compare observed and model-calculated percentage particulate Cu and Zn as influenced by sorption to both HFO and HMO and aqueous complexation with dissolved organic carbon (DOC). Over distance, Cu and Fe were transported predominantly in the particulate phase, Mn in the dissolved phase, and Zn was intermediate in its distribution, with generally about 50% being in each phase. Under higher flows, a larger fraction of the total metals was present in the dissolved phase, along with a lower total suspended sediment (TSS) concentration. This is consistent with the source of TSS being predominantly in-stream precipitation of metals, which might be kinetically limited under higher flows. Modeling results most closely represented observed percentage particulate Cu under lower flows; a strong seasonal trend was not evident for Zn. Model over-predictions of percentage particulate Cu suggest non-equilibrium with sorbent phases or that something in addition to DOC was keeping a portion of the Cu in solution; under-predictions for Zn suggest an additional sorbent. Differences between observed and modeled particulate varied significantly between sites and seasons; ranging from 1 to 54% for Cu and 1 to 34% for Zn overall.

  12. Sustainable watershed management: an international multi-watershed case study.

    PubMed

    Wagner, Walter; Gawel, James; Furuma, Hiroak; De Souza, Marcelo Pereira; Teixeira, Denilson; Rios, Leonardo; Ohgaki, Shinichiro; Zehnder, Alexander J B; Hemond, Harold F

    2002-02-01

    Global freshwater resources are being increasingly polluted and depleted, threatening sustainable development and human and ecosystem health. Utilizing case studies from 4 different watersheds in the United States, Japan, Switzerland, and Brazil, this paper identifies the most relevant sustainability deficits and derives general vectors for more sustainable water management. As a consequence of the demographic and economic developments experienced in the last few decades, each watershed has suffered declines in water quality, streamflow and biotic resources. However, the extent and the cultural perception of these water-related problems vary substantially in the different watersheds, leading to specific water-management strategies. In industrialized countries, exemplified by the US, Switzerland, and Japan, these strategies have primarily consisted of finance- and energy-intensive technologies, allowing these countries to meet water requirements while minimizing human health risks. But, from a sustainability point of view, such strategies, relying on limited natural resources, are not long-term solutions. For newly industrialized countries such as Brazil, expensive technologies for water management are often not economically feasible, thus limiting the extent to which newly industrialized and developing countries can utilize the expertise offered by the industrialized world. Sustainable water management has to be achieved by a common learning process involving industrialized, newly industrialized, and developing countries, following general sustainability guidelines as exemplified in this paper.

  13. Quartz tuning-fork oscillations in He II and drag coefficient

    NASA Astrophysics Data System (ADS)

    Gritsenko, I. A.; Zadorozhko, A. A.; Neoneta, A. S.; Chagovets, V. K.; Sheshin, G. A.

    2011-07-01

    The temperature dependencies of drag coefficient for quartz tuning forks of various geometric dimensions, immersed in the He II, were determined experimentally in the temperature range 0.1-3 K. It is identified, that these dependencies are similar, but the values of drag coefficient are different for tuning forks with different geometric dimensions. It is shown, that the obtained specific drag coefficient depends only on the temperature and frequency of vibrations, when the value of drag coefficient is normalized to the surface area of moving tuning-fork prong. The temperature dependencies of normalized drag coefficient for the tuning forks of various dimensions, wire, and microsphere, oscillating in the Не II, are compared. It is shown, that in the ballistic regime of scattering of quasiparticles, these dependencies are identical and have a slope proportional to T4, which is determined by the density of thermal excitations. In the hydrodynamic regime at T > 0.5 K, the behavior of the temperature dependence of specific drag coefficient is affected by the size and frequency of vibrating body. The empirical relation, which allows to describe the behavior of specific drag coefficient for vibrating tuning forks, microsphere, and wire everywhere over the temperature region and at various frequencies, is proposed.

  14. Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells

    PubMed Central

    Kaushik Tiwari, Meetu; Adaku, Nneoma; Peart, Natoya; Rogers, Faye A.

    2016-01-01

    Structural alterations in DNA can serve as natural impediments to replication fork stability and progression, resulting in DNA damage and genomic instability. Naturally occurring polypurine mirror repeat sequences in the human genome can create endogenous triplex structures evoking a robust DNA damage response. Failures to recognize or adequately process these genomic lesions can result in loss of genomic integrity. Nucleotide excision repair (NER) proteins have been found to play a prominent role in the recognition and repair of triplex structures. We demonstrate using triplex-forming oligonucleotides that chromosomal triplexes perturb DNA replication fork progression, eventually resulting in fork collapse and the induction of double strand breaks (DSBs). We find that cells deficient in the NER damage recognition proteins, XPA and XPC, accumulate more DSBs in response to chromosomal triplex formation than NER-proficient cells. Furthermore, we demonstrate that XPC-deficient cells are particularly prone to replication-associated DSBs in the presence of triplexes. In the absence of XPA or XPC, deleterious consequences of triplex-induced genomic instability may be averted by activating apoptosis via dual phosphorylation of the H2AX protein. Our results reveal that damage recognition by XPC and XPA is critical to maintaining replication fork integrity and preventing replication fork collapse in the presence of triplex structures. PMID:27298253

  15. NEK8 regulates DNA damage-induced RAD51 foci formation and replication fork protection

    PubMed Central

    Abeyta, Antonio; Castella, Maria; Jacquemont, Celine; Taniguchi, Toshiyasu

    2017-01-01

    ABSTRACT Proteins essential for homologous recombination play a pivotal role in the repair of DNA double strand breaks, DNA inter-strand crosslinks and replication fork stability. Defects in homologous recombination also play a critical role in the development of cancer and the sensitivity of these cancers to chemotherapy. RAD51, an essential factor for homologous recombination and replication fork protection, accumulates and forms immunocytochemically detectable nuclear foci at sites of DNA damage. To identify kinases that may regulate RAD51 localization to sites of DNA damage, we performed a human kinome siRNA library screen, using DNA damage-induced RAD51 foci formation as readout. We found that NEK8, a NIMA family kinase member, is required for efficient DNA damage-induced RAD51 foci formation. Interestingly, knockout of Nek8 in murine embryonic fibroblasts led to cellular sensitivity to the replication inhibitor, hydroxyurea, and inhibition of the ATR kinase. Furthermore, NEK8 was required for proper replication fork protection following replication stall with hydroxyurea. Loading of RAD51 to chromatin was decreased in NEK8-depleted cells and Nek8-knockout cells. Single-molecule DNA fiber analyses revealed that nascent DNA tracts were degraded in the absence of NEK8 following treatment with hydroxyurea. Consistent with this, Nek8-knockout cells showed increased chromosome breaks following treatment with hydroxyurea. Thus, NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51. PMID:27892797

  16. South Fork Snake River/Palisades Wildlife Mitigation Project: Environmental assessment

    SciTech Connect

    1995-09-01

    BPA proposes to fund the implementation of the South Fork Snake River Programmatic Management Plan to compensate for losses of wildlife and wildlife habitat due to hydroelectric development at Palisades Dam. The Idaho Department of Fish and Game drafted the plan, which was completed in May 1993. This plan recommends land and conservation easement acquisition and wildlife habitat enhancement measures. These measures would be implemented on selected lands along the South Fork of the Snake River between Palisades Dam and the confluence with the Henry`s Fork, and on portions of the Henry`s Fork located in Bonneville, Madison, and Jefferson Counties, Idaho. BPA has prepared an Environmental Assessment evaluating the proposed project. The EA also incorporates by reference the analyses in the South Fork Snake River Activity/Operations Plan and EA prepared jointly in 1991 by the Bureau of Land Management and the Forest Service. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an Environmental Impact Statement (EIS) is not required and BPA is issuing this FONSI.

  17. Biological Monitoring Program for East Fork Poplar Creek

    SciTech Connect

    Adams, S.M.; Christensen, S.W.; Greeley, M.S.jr; Hill, W.R.; Kszos, L.A.; McCarthy, J.F.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.

    1998-10-15

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biologicai Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the compiex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC, These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumuiation studies, and (4) ecological surveys of the periphyton, benthic macro invertebrate, and fish communities. Monitoring is currently being conducted at five sites, although sites maybe excluded and/or others added depending upon the specific objectives of the various tasks. Criteria used in selecting the sites include: (1) location of sampling sites used in other studies, (2) known or suspected sources of downstream impacts, (3) proximity to U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR) boundaries, (4) concentration of mercury in the adjacent floodplain, (5) appropriate habitat distribution, and (6

  18. Analysis of sensitivity of simulated recharge to selected parameters for seven watersheds modeled using the precipitation-runoff modeling system

    USGS Publications Warehouse

    Ely, D. Matthew

    2006-01-01

    Recharge is a vital component of the ground-water budget and methods for estimating it range from extremely complex to relatively simple. The most commonly used techniques, however, are limited by the scale of application. One method that can be used to estimate ground-water recharge includes process-based models that compute distributed water budgets on a watershed scale. These models should be evaluated to determine which model parameters are the dominant controls in determining ground-water recharge. Seven existing watershed models from different humid regions of the United States were chosen to analyze the sensitivity of simulated recharge to model parameters. Parameter sensitivities were determined using a nonlinear regression computer program to generate a suite of diagnostic statistics. The statistics identify model parameters that have the greatest effect on simulated ground-water recharge and that compare and contrast the hydrologic system responses to those parameters. Simulated recharge in the Lost River and Big Creek watersheds in Washington State was sensitive to small changes in air temperature. The Hamden watershed model in west-central Minnesota was developed to investigate the relations that wetlands and other landscape features have with runoff processes. Excess soil moisture in the Hamden watershed simulation was preferentially routed to wetlands, instead of to the ground-water system, resulting in little sensitivity of any parameters to recharge. Simulated recharge in the North Fork Pheasant Branch watershed, Wisconsin, demonstrated the greatest sensitivity to parameters related to evapotranspiration. Three watersheds were simulated as part of the Model Parameter Estimation Experiment (MOPEX). Parameter sensitivities for the MOPEX watersheds, Amite River, Louisiana and Mississippi, English River, Iowa, and South Branch Potomac River, West Virginia, were similar and most sensitive to small changes in air temperature and a user-defined flow

  19. Regional Ecorisk Field investigation, upper Clark Fork River Basin

    SciTech Connect

    Pastorok, R.; LaTier, A.; Ginn, T.

    1995-12-31

    The Regional Ecorisk Field Investigation was conducted at the Clark Fork River Superfund Site (Montana) to evaluate the relationships between plant communities and tailings deposits in riparian habitats and to evaluate food-chain transfer of trace elements to selected wildlife species. Stations were selected to represent a range of vegetation biomass (or cover) values and apparent impact of trace elements, with some areas of lush vegetation, some areas of mostly unvegetated soil (e.g., < 30 percent plant cover), and a gradient in between. For the evaluation of risk to wildlife, bioaccumulation of metals was evaluated in native or naturalized plants, terrestrial invertebrates, and the deer mouse (Peromyscus maniculatus). Potential reproductive effects in the deer mouse were evaluated by direct measurements. For other wildlife species, bioaccumulation data were interpreted in the context of food web exposure models. Total biomass and species richness of riparian plant communities are related to tailings content of soil as indicated by pH and metals concentrations. Risk to populations of omnivorous small mammals such as the deer mouse was not significant. Relative abundance and reproductive condition of the deer mouse were normal, even in areas of high metals enrichment. Based on exposure models and site-specific tissue residue data for dietary species, risk to local populations of predators such as red fox and American kestrel that feed on deer mice and terrestrial invertebrates is not significant. Risk to herbivores related to metals bioaccumulation in plant tissues is not significant. Population level effects in deer and other large wildlife are not expected because of the large home ranges of such species and compensatory demographic factors.

  20. Soils of Walker Branch Watershed

    SciTech Connect

    Lietzke, D.A.

    1994-01-01

    The soil survey of Walker Branch Watershed (WBW) utilized the most up-to-date knowledge of soils, geology, and geohydrology in building the soils data base needed to reinterpret past research and to begin new research in the watershed. The soils of WBW were also compared with soils mapped elsewhere along Chestnut Ridge on the Oak Ridge Reservation to (1) establish whether knowledge obtained elsewhere could be used within the watershed, (2) determine whether there were any soils restricted to the watershed, and (3) evaluate geologic formation lateral variability. Soils, surficial geology, and geomorphology were mapped at a scale of 1:1200 using a paper base map having 2-ft contour intervals. Most of the contours seemed to reasonably represent actual landform configurations, except for dense wooded areas. For example, the very large dolines or sinkholes were shown on the contour base map, but numerous smaller ones were not. In addition, small drainageways and gullies were often not shown. These often small but important features were located approximately as soil mapping progressed. WBW is underlain by dolostones of the Knox Group, but only a very small part of the surface area contains outcroppings of rock and most outcrops were located in the lower part. Soil mapping revealed the presence of both ancient alluvium and ancient colluvium deposits, not recognized in previous soil surveys, that have been preserved in high-elevation stable portions of present-day landforms. An erosional geomorphic process of topographic inversion requiring several millions of years within the Pleistocene is necessary to bring about the degree of inversion that is expressed in the watershed. Indeed, some of these ancient alluvial and colluvial remnants may date back into the Tertiary. Also evident in the watershed, and preserved in the broad, nearly level bottoms of dolines, are multiple deposits of silty material either devoid or nearly devoid of coarse fragments. Recent research

  1. A Watershed Integrity Definition and Assessment Approach to Support Strategic Management of Watersheds

    EPA Science Inventory

    Although defined hydrologically as a drainage basin, watersheds are systems that physically link the individual social and ecological attributes that comprise them. Hence the structure, function, and feedback systems of watersheds are dependent on interactions between these soci...

  2. Field Studies of Streamflow Generation Using Natural and Injected Tracers on Bickford and Walker Branch Watersheds

    SciTech Connect

    Genereux, D.

    1992-01-01

    Field studies of streamflow generation were undertaken on two forested watersheds, the West Road subcatchment of Bickford Watershed in central Massachusetts and the West Fork of Walker Branch Watershed in eastern Tennessee. A major component of the research was development of a two-stage methodology for the use of naturally-occurring {sup 222}Rn as a tracer. The first of the two stages was solving a mass-balance equation for {sup 222}Rn around a stream reach of interest in order to calculate [Rn]{sub q}, the {sup 222}Rn content of the lateral inflow to the reach; a conservative tracer (chloride) and a volatile tracer (propane) were injected into the study stream to account for lateral inflow to, and {sup 222}Rn volatilization from, the study reach. The second stage involved quantitative comparison of [Rn]{sub q} to the measured {sup 222}Rn concentrations of different subsurface waters in order to assess how important these waters were in contributing lateral inflow to the stream reach. The method was first applied to a 34 m stream reach at Bickford during baseflow; results suggested that {ge} 70% of the lateral inflow could be considered vadose zone water (water which had been in a saturated zone for less than a few days), and the remainder ''soil groundwater'' or ''saturated zone water'' (which had a longer residence time in a soil saturated zone). The method was then applied to two stream reaches on the West Fork of Walker Branch over a wide range of flow conditions; four springs were also investigated. It was found that springwater and inflow to the stream could be viewed as a mixture of water from three end members: the two defined at Bickford (vadose zone water and soil groundwater) and a third (bedrock groundwater) to account for the movement of water through fractured dolomite bedrock. Calcium was used as a second naturally-occurring tracer to distinguish bedrock groundwater from the other two end members. The behavior indicated by the three

  3. Multiple critical velocities in oscillatory flow of superfluid 4He due to quartz tuning forks

    NASA Astrophysics Data System (ADS)

    Schmoranzer, D.; Jackson, M. J.; Tsepelin, V.; Poole, M.; Woods, A. J.; Človečko, M.; Skrbek, L.

    2016-12-01

    We report recent investigations into the transition to turbulence in superfluid 4He, realized experimentally by measuring the drag forces acting on two custom-made quartz tuning forks with fundamental resonances at 6.5 kHz and 55.5 kHz, in the temperature range 10 mK to 2.17 K. In pure superfluid in the zero temperature limit, three distinct critical velocities were observed with both tuning forks. We discuss the significance of all critical velocities and associate the third critical velocity reported here with the development of large vortical structures in the flow, which thus starts to mimic turbulence in classical fluids. The interpretation of our results is directly linked to previous experimental work with oscillators such as tuning forks, grids, and vibrating wires, focusing on the behavior of purely superfluid 4He at very low temperatures.

  4. Replication Fork Protection Factors Controlling R-Loop Bypass and Suppression

    PubMed Central

    Chang, Emily Yun-Chia; Stirling, Peter C.

    2017-01-01

    Replication–transcription conflicts have been a well-studied source of genome instability for many years and have frequently been linked to defects in RNA processing. However, recent characterization of replication fork-associated proteins has revealed that defects in fork protection can directly or indirectly stabilize R-loop structures in the genome and promote transcription–replication conflicts that lead to genome instability. Defects in essential DNA replication-associated activities like topoisomerase, or the minichromosome maintenance (MCM) helicase complex, as well as fork-associated protection factors like the Fanconi anemia pathway, both appear to mitigate transcription–replication conflicts. Here, we will highlight recent advances that support the concept that normal and robust replisome function itself is a key component of mitigating R-loop coupled genome instability. PMID:28098815

  5. FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery.

    PubMed

    Chaudhury, Indrajit; Sareen, Archana; Raghunandan, Maya; Sobeck, Alexandra

    2013-07-01

    Fanconi Anemia (FA) and Bloom Syndrome share overlapping phenotypes including spontaneous chromosomal abnormalities and increased cancer predisposition. The FA protein pathway comprises an upstream core complex that mediates recruitment of two central players, FANCD2 and FANCI, to sites of stalled replication forks. Successful fork recovery depends on the Bloom's helicase BLM that participates in a larger protein complex ('BLMcx') containing topoisomerase III alpha, RMI1, RMI2 and replication protein A. We show that FANCD2 is an essential regulator of BLMcx functions: it maintains BLM protein stability and is crucial for complete BLMcx assembly; moreover, it recruits BLMcx to replicating chromatin during normal S-phase and mediates phosphorylation of BLMcx members in response to DNA damage. During replication stress, FANCD2 and BLM cooperate to promote restart of stalled replication forks while suppressing firing of new replication origins. In contrast, FANCI is dispensable for FANCD2-dependent BLMcx regulation, demonstrating functional separation of FANCD2 from FANCI.

  6. Maps showing mines, quarries, prospects, and exposures in the Devils Fork Roadless Area, Scott County, Virginia

    USGS Publications Warehouse

    Behum, Paul T.

    1984-01-01

    The Devils Fork Roadless Area is located at the eastern edge of the Appalachian coal region and is within the Cumberland Mountain section of the Appalachian Plateau physiographic province. Most of the area is drained by Devil Fork and its tributaries. Clinch Rock Branch of Straight Creek, Roddy Branch of Valley Creek, and Stinking Creek, all tributary to the Clinch River, drain small fringe tracts. Altitudes range from about 1,550 ft on the lower part of Straight Fork to about 3,490 ft at Cox Place on Little Mountain. Vegetation varies from mixed hardwoods in the uplands to thickets of conifer, rhododendron, and laurel in moist protected areas, as in coves along drainage courses.

  7. Replication Fork Protection Factors Controlling R-Loop Bypass and Suppression.

    PubMed

    Chang, Emily Yun-Chia; Stirling, Peter C

    2017-01-14

    Replication-transcription conflicts have been a well-studied source of genome instability for many years and have frequently been linked to defects in RNA processing. However, recent characterization of replication fork-associated proteins has revealed that defects in fork protection can directly or indirectly stabilize R-loop structures in the genome and promote transcription-replication conflicts that lead to genome instability. Defects in essential DNA replication-associated activities like topoisomerase, or the minichromosome maintenance (MCM) helicase complex, as well as fork-associated protection factors like the Fanconi anemia pathway, both appear to mitigate transcription-replication conflicts. Here, we will highlight recent advances that support the concept that normal and robust replisome function itself is a key component of mitigating R-loop coupled genome instability.

  8. South Fork Clearwater River Habitat Enhancement, Crooked and Red Rivers : Annual Report, 1989.

    SciTech Connect

    Baer, William H.

    1990-01-01

    In 1983, the Nez Perce National Forest and the Bonneville Power Administration entered into an interagency agreement to enhance and improve habitat for two anadromous fish species, spring chinook salmon (Oncorhynchus tshawyscha) and summer steelhead trout (Onchorhyncus mykiss), in the South Fork Clearwater River tributaries. The South Fork Clearwater River was dammed in 1927 for hydroelectric development. Anadromous fish runs were virtually eliminated until the dam was removed in 1962. To complicate the problem, upstream spawning and rearing habitats were severely impacted by dredge and hydraulic mining, road building, timber harvest, and over-grazing. Fish habitat improvement projects under the above contract are being carried out in two major tributaries to the South Fork Clearwater River. Both the Red River and the Crooked River projects began in 1983 and will be completed in 1990. 12 figures., 1 tab.

  9. Pathological Changes of von Economo Neuron and Fork Neuron in Neuropsychiatric Diseases.

    PubMed

    Liu, Jia; Wang, Lu-ning; Arzberger, Thomas; Zhu, Ming-wei

    2016-02-01

    von Economo neuron (VEN) is a bipolar neuron characterized by a large spindle-shaped soma. VEN is generally distributed in the layer V of anterior insular lobe and anterior cingulate cortex. Fork neuron is another featured bipolar neuron. In recent years,many studies have illustrated that VEN and fork neurons are correlated with complicated cognition such as self-consciousness and social emotion. Studies in the development and morpholigies of these two neurons as well as their pathological changes in various neurological and psychiatric disorders have found that the abnormal number and functions of VEN can cause corresponding dysfunctions in social recognition and emotions both during the neuro-developmental stages of childhood and during the nerve degeneration in old age stage. Therefore, more attentions should be paid on the research of VEN and fork neurons in neuropsychiatric diseases.

  10. Introduction to the Watershed Central Web Site and Watershed Wiki Mini-Workshop

    EPA Science Inventory

    Many communities across the country struggle to find the right approaches, tools and data to include in their watershed plans. EPA recently posted a new web site called "Watershed Central,” a “one-stop" tool, to help watershed organizations and others find key resources to protec...

  11. Engaging Watershed Stakeholders for Cost-Effective Environmental Management Planning with "Watershed Manager"

    ERIC Educational Resources Information Center

    Williams, Jeffery R.; Smith, Craig M.; Roe, Josh D.; Leatherman, John C.; Wilson, Robert M.

    2012-01-01

    "Watershed Manager" is a spreadsheet-based model that is used in extension education programs for learning about and selecting cost-effective watershed management practices to reduce soil, nitrogen, and phosphorus losses from cropland. It can facilitate Watershed Restoration and Protection Strategy (WRAPS) stakeholder groups' development…

  12. Watershed Stewardship Education Program--A Multidisciplinary Extension Education Program for Oregon's Watershed Councils.

    ERIC Educational Resources Information Center

    Conway, Flaxen D. L.; Godwin, Derek; Cloughesy, Mike; Nierenberg, Tara

    2003-01-01

    The Watershed Stewardship Education Program (WSEP) is a multidisciplinary Oregon Extension designed to help watershed councils, landowners, and others work effectively together on water management. Components include practical, easy-to-use educational materials, training in effective collaboration, a Master Watershed Stewards program, and advanced…

  13. Volunteer Watershed Health Monitoring by Local Stakeholders: New Mexico Watershed Watch

    ERIC Educational Resources Information Center

    Fleming, William

    2003-01-01

    Volunteers monitor watershed health in more than 700 programs in the US, involving over 400,000 local stakeholders. New Mexico Watershed Watch is a student-based watershed monitoring program sponsored by the state's Department of Game and Fish which provides high school teachers and students with instruction on methods for water quality…

  14. A TEST OF WATERSHED CLASSIFICATION SYSTEMS FOR ECOLOGICAL RISK ASSESSMENT

    EPA Science Inventory

    To facilitate extrapolation among watersheds, ecological risk assessments should be based on a model of underlying factors influencing watershed response, particularly vulnerability. We propose a conceptual model of landscape vulnerability to serve as a basis for watershed classi...

  15. AN INTEGRATED COASTAL-WATERSHED MONITORING FRAMEWORK FOR ASSESSMENT

    EPA Science Inventory

    An approach for watershed classification in support of assessments, disgnosis of biological impairment, and prioritization of watershed restorations has been tested in coastal watersheds surrounding the western arm of Lake Superior and is currently being assessed for a series of ...

  16. Applications of remote sensing to watershed management

    NASA Technical Reports Server (NTRS)

    Rango, A.

    1975-01-01

    Aircraft and satellite remote sensing systems which are capable of contributing to watershed management are described and include: the multispectral scanner subsystem on LANDSAT and the basic multispectral camera array flown on high altitude aircraft such as the U-2. Various aspects of watershed management investigated by remote sensing systems are discussed. Major areas included are: snow mapping, surface water inventories, flood management, hydrologic land use monitoring, and watershed modeling. It is indicated that technological advances in remote sensing of hydrological data must be coupled with an expansion of awareness and training in remote sensing techniques of the watershed management community.

  17. 76 FR 50171 - Notice of Intent To Prepare an Environmental Impact Statement for the Henrys Fork Salinity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-12

    ... the Henrys Fork Salinity Control Project Plan, Sweetwater and Uinta Counties, WY; Daggett and Summit... Statement (EIS) for the Henrys Fork Salinity Control Project Plan (SCPP). The NRCS will be the lead agency... Improvements'' alternative assumes a salinity control project will be implemented. Existing financial...

  18. 77 FR 66865 - Record of Decision for the Oil and Gas Management Plan, Big South Fork National River and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... National Park Service Record of Decision for the Oil and Gas Management Plan, Big South Fork National River... (ROD) for the Oil and Gas Management Plan (Plan) for Big South Fork National River and Recreation Area and Obed Wild and Scenic River. On September 5, 2012, the Southeast Regional Director, approved...

  19. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  20. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  1. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  2. HLTF’s Ancient HIRAN Domain Binds 3′-DNA Ends to Drive Replication Fork Reversal

    PubMed Central

    Kile, Andrew C.; Chavez, Diana A.; Bacal, Julien; Eldirany, Sherif; Korzhnev, Dmitry M.; Bezsonova, Irina; Eichman, Brandt F.; Cimprich, Karlene A.

    2015-01-01

    Summary Stalled replication forks are a critical problem for the cell because they can lead to complex genome rearrangements that underlie cell death and disease. Processes such as DNA damage tolerance and replication fork reversal protect stalled forks from these events. A central mediator of these DNA damage responses in humans is the Rad5-related DNA translocase, HLTF. Here, we present biochemical and structural evidence that the HIRAN domain, an ancient and conserved domain found in HLTF and other DNA processing proteins, is a modified oligonucleotide/oligosaccharide (OB) fold that binds to 3′-ssDNA ends. We demonstrate that the HIRAN domain promotes HLTF-dependent fork reversal in vitro, through its interaction with 3′-ssDNA ends found at forks. Finally, we show that HLTF restrains replication fork progression in cells in a HIRAN-dependent manner. These findings establish a mechanism of HLTF-mediated fork reversal and provide insight into the requirement for distinct fork remodeling activities in the cell. PMID:26051180

  3. Integrating Salmon Recovery, Clean Water Act Compliance, Restoration, and Climate Change Impacts in the South Fork Nooksack River

    EPA Science Inventory

    "The South Fork Nooksack River (SFNR) is an important tributary to the Nooksack River, Bellingham Bay, and the Salish Sea. The South Fork Nooksack River comprises one of the 22 independent populations of spring Chinook in the Puget Sound Chinook Evolutionarily Significant Un...

  4. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  5. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  6. Recombination occurs within minutes of replication blockage by RTS1 producing restarted forks that are prone to collapse

    PubMed Central

    Nguyen, Michael O; Jalan, Manisha; Morrow, Carl A; Osman, Fekret; Whitby, Matthew C

    2015-01-01

    The completion of genome duplication during the cell cycle is threatened by the presence of replication fork barriers (RFBs). Following collision with a RFB, replication proteins can dissociate from the stalled fork (fork collapse) rendering it incapable of further DNA synthesis unless recombination intervenes to restart replication. We use time-lapse microscopy and genetic assays to show that recombination is initiated within ∼10 min of replication fork blockage at a site-specific barrier in fission yeast, leading to a restarted fork within ∼60 min, which is only prevented/curtailed by the arrival of the opposing replication fork. The restarted fork is susceptible to further collapse causing hyper-recombination downstream of the barrier. Surprisingly, in our system fork restart is unnecessary for maintaining cell viability. Seemingly, the risk of failing to complete replication prior to mitosis is sufficient to warrant the induction of recombination even though it can cause deleterious genetic change. DOI: http://dx.doi.org/10.7554/eLife.04539.001 PMID:25806683

  7. Using bioenergetics modeling to estimate consumption of native juvenile salmonids by nonnative northern pike in the Upper Flathead River System, Montana

    USGS Publications Warehouse

    Muhlfeld, C.C.; Bennett, D.H.; Kirk, Steinhorst R.; Marotz, B.; Boyer, M.

    2008-01-01

    Introductions of nonnative northern pike Esox lucius have created recreational fisheries in many waters in the United States and Canada, yet many studies have shown that introduced northern pike may alter the composition and structure of fish communities through predation. We estimated the abundance of nonnative northern pike (2002-2003) and applied food habits data (1999-2003) to estimate their annual consumption of native bull trout Salvelinus confluentus and westslope cutthroat trout Oncorhynchus clarkii lewisi juveniles in the upper Flathead River system, Montana. Population estimates were generally consistent among years and ranged from 1,200 to 1,300 individuals. Westslope cutthroat trout were present in the diet of younger (???600 mm) and older (>600 mm) northern pike during all seasons and bull trout were found only in larger northern pike during all seasons but summer. Bioenergetics modeling estimated that the northern pike population annually consumed a total of 8.0 metric tons (mt) of fish flesh; the highest biomass was composed of cyprinids (4.95 mt) followed by whitefishes Prosopium spp. (1.02 mt), bull trout (0.80 mt), westslope cutthroat trout (0.68 mt), yellow perch Perca flavescens (0.41 mt),1 and other fishes (centrarchids and cottids; 0.14 mt). Numerically, the northern pike population consumed more than 342,000 fish; cyprinids and catostomids comprised approximately 82% of prey fish (278,925), whereas over 13,000 westslope cutthroat trout and nearly 3,500 bull trout were eaten, comprising about 5% of the prey consumed. Our results suggest that predation by introduced northern pike is contributing to the lower abundance of native salmonids in the system and that a possible benefit might accrue to native salmonids by reducing these predatory interactions. ?? Copyright by the American Fisheries Society 2008.

  8. Geohydrologic appraisal of water resources of the South Fork, Long Island, New York

    USGS Publications Warehouse

    Nemickas, Bronius; Koszalka, Edward J.

    1982-01-01

    The ground-water resources of the South Fork of Long Island, N.Y., were investigated from April 1974 to September 1977. The study area encompasses 137 square miles and includes the eastern part of the Town of Southampton and the entire Town of East Hampton. The South Fork consists of a Paleozoic basement complex that is overlain by Cretaceous and Pleistocene sediments. The surficial material is composed of Late Wisconsinan glacial and glaciofluvial deposits in association with beach and marsh deposits of Recent age. Till underlies most of the eastern part of the South Fork. Precipitation is the sole source of fresh ground water on the South Fork. Average annual precipitation recorded at Bridgehampton from 1931-76 is 45 inches; about half this amount reaches the ground-water reservoir. It is estimated that overland runoff amounts to 0.5 inches per year, and evapotranspiration is 23 inches per year. Thus, recharge equals approximately 22 inches per year. Hydraulic conductivity and transmissivity of the Magothy (Cretaceous) and upper glacial (Pleistocene) aquifers on the South Fork were estimated from aquifer tests and specific-capacity data. The average horizontal hydraulic conductivity of the Magothy aquifer is 70 feet per day, and of the upper glacial aquifer 350 feet per day. Transmissivity of the Magothy aquifer on the South Fork ranges from 600 to 24,100 feet squared per day; transmissivity of the upper glacial aquifer ranges from 5,400 feet to 22,700 feet squared per day. No potable water is available from the underlying Lloyd aquifer. The position of the freshwater to saline-water interface is depicted in maps. In the southern part of the area, the freshwater reservoir follows the Ghyben-Herzberg principle, but in the northern part, the depth to interface is less than expected owing to a greater degree of anisotropy of the geologic units. Total public-supply pumpage on the South Fork is estimated to be about 3 Mgal/day, (million gallons per day). Public

  9. Regulating telomere length from the inside out: the replication fork model

    PubMed Central

    2016-01-01

    Telomere length is regulated around an equilibrium set point. Telomeres shorten during replication and are lengthened by telomerase. Disruption of the length equilibrium leads to disease; thus, it is important to understand the mechanisms that regulate length at the molecular level. The prevailing protein-counting model for regulating telomerase access to elongate the telomere does not explain accumulating evidence of a role of DNA replication in telomere length regulation. Here I present an alternative model: the replication fork model that can explain how passage of a replication fork and regulation of origin firing affect telomere length. PMID:27401551

  10. The Sound Field Around a Tuning Fork and the Role of a Resonance Box

    NASA Astrophysics Data System (ADS)

    Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

    2015-02-01

    Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: Why is a bare tuning fork such a weak emitter of sound?What is the role of the resonance box?Where does energy connected with larger intensity of emitted acoustic waves come from?

  11. Endonuclease cleavage of blocked replication forks: An indirect pathway of DNA damage from antitumor drug-topoisomerase complexes

    NASA Astrophysics Data System (ADS)

    Hong, George; Kreuzer, Kenneth N.

    2003-04-01

    The cytotoxicity of several important antitumor drugs depends on formation of the covalent topoisomerase-DNA cleavage complex. However, cellular processes such as DNA replication are necessary to convert the cleavage complex into a cytotoxic lesion, but the molecular mechanism of this conversion and the precise nature of the cytotoxic lesion are unknown. Using a bacteriophage T4 model system, we have previously shown that antitumor drug-induced cleavage complexes block replication forks in vivo. In this report, we show that these blocked forks can be cleaved by T4 endonuclease VII to create overt DNA breaks. The accumulation of blocked forks increased in endonuclease VII-deficient infections, suggesting that endonuclease cleavage contributes to fork processing in vivo. Furthermore, purified endonuclease VII cleaved the blocked forks in vitro close to the branch points. These results suggest that an indirect pathway of branched-DNA cleavage contributes to the cytotoxicity of antitumor drugs that target DNA topoisomerases.

  12. A Stochastic Multi-Media Model of Microbial Transport in Watersheds

    NASA Astrophysics Data System (ADS)

    Yeghiazarian, L.; Safwat, A.; Whiteaker, T.; Teklitz, A.; Nietch, C.; Maidment, D. R.; Best, E. P.

    2012-12-01

    Fecal contamination is the leading cause of surface-water impairment in the US, and fecal pathogens are capable of triggering massive outbreaks of gastrointestinal disease. The difficulty in prediction of water contamination has its roots in the stochastic variability of fecal pathogens in the environment, and in the complexity of microbial dynamics and interactions on the soil surface and in water. To address these challenges, we have developed a stochastic model whereby the transport of microorganisms in watersheds is considered in two broad categories: microorganisms that are attached to mineral or organic substrates in suspended sediment; and unattached microorganisms suspended in overland flow. The interactions of microorganisms with soil particles on the soil surface and in the overland flow lead to transitions of microorganisms between solid and aqueous media. The strength of attachment of microorganisms to soil particles is determined by the chemical characteristics of soils which are highly correlated with the particle size. The particle size class distribution in the suspended sediment is predicted by the Water Erosion Prediction Project (WEPP). The model is integrated with ArcGIS, resulting in a general transport-modeling framework applicable to a variety of biological and chemical surface water contaminants. Simulations are carried out for a case study of contaminant transport in the East Fork Little Miami River Watershed in Ohio. Model results include the spatial probability distribution of microbes in the watershed and can be used for assessment of (1) mechanisms dominating microbial transport, and (2) time and location of highest likelihood of microbial occurrence, thus yielding information on best water sampling strategies.

  13. EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair

    PubMed Central

    Wu, Yuehan; Lee, Suk-Hee; Williamson, Elizabeth A.; Reinert, Brian L.; Cho, Ju Hwan; Xia, Fen; Jaiswal, Aruna Shanker; Srinivasan, Gayathri; Patel, Bhavita; Brantley, Alexis; Zhou, Daohong; Shao, Lijian; Pathak, Rupak; Hauer-Jensen, Martin; Singh, Sudha; Kong, Kimi; Wu, Xaiohua; Kim, Hyun-Suk; Beissbarth, Timothy; Gaedcke, Jochen; Burma, Sandeep; Nickoloff, Jac A.; Hromas, Robert A.

    2015-01-01

    Replication fork stalling and collapse is a major source of genome instability leading to neoplastic transformation or cell death. Such stressed replication forks can be conservatively repaired and restarted using homologous recombination (HR) or non-conservatively repaired using micro-homology mediated end joining (MMEJ). HR repair of stressed forks is initiated by 5’ end resection near the fork junction, which permits 3’ single strand invasion of a homologous template for fork restart. This 5’ end resection also prevents classical non-homologous end-joining (cNHEJ), a competing pathway for DNA double-strand break (DSB) repair. Unopposed NHEJ can cause genome instability during replication stress by abnormally fusing free double strand ends that occur as unstable replication fork repair intermediates. We show here that the previously uncharacterized Exonuclease/Endonuclease/Phosphatase Domain-1 (EEPD1) protein is required for initiating repair and restart of stalled forks. EEPD1 is recruited to stalled forks, enhances 5’ DNA end resection, and promotes restart of stalled forks. Interestingly, EEPD1 directs DSB repair away from cNHEJ, and also away from MMEJ, which requires limited end resection for initiation. EEPD1 is also required for proper ATR and CHK1 phosphorylation, and formation of gamma-H2AX, RAD51 and phospho-RPA32 foci. Consistent with a direct role in stalled replication fork cleavage, EEPD1 is a 5’ overhang nuclease in an obligate complex with the end resection nuclease Exo1 and BLM. EEPD1 depletion causes nuclear and cytogenetic defects, which are made worse by replication stress. Depleting 53BP1, which slows cNHEJ, fully rescues the nuclear and cytogenetic abnormalities seen with EEPD1 depletion. These data demonstrate that genome stability during replication stress is maintained by EEPD1, which initiates HR and inhibits cNHEJ and MMEJ. PMID:26684013

  14. Recent sedimentation and surface-water flow patterns on the flood plain of the North Fork Forked Deer River, Dyer County, Tennessee

    USGS Publications Warehouse

    Wolfe, W.J.; Diehl, T.H.

    1993-01-01

    Sedimentation in the 19th and 20th centuries has had a major effect on surface-water drainage conditions along a 7-mile section of the North, Fork Forked Deer River flood plain, Dyer County, Tenn. During the century prior to 1930, 5 to 12 feet of sediment were deposited over much of the flood plain, resulting in channel obstruction and widespread flooding. The estimated bankfull capacity of the natural channel before it was channelized in 19 16 was comparable to the base flow of the river during the 1980's. Ditching of the river between 191i6 and 1;9,21 was followed by reductions in sedimentation rates over parts of the flood plain. However, the effects of sedimentation have persisted. Occlusions along the natural channel of the river have divided this stream reach into a series of sloughs. These sloughs continue to fill with sediment and are surrounded by ponds that have expanded since 1941. Degradation of the North Fork Forked Deer ditch may eventually reduce ponding over much of the flood plain. Active incision of headcuts in both banks of the ditch is enhancing the drainage of widespread ponded areas. These headcuts likely will have limited effect on drainage of most tributaries. The highest recent sedimentation rates, in places more than 0.2 foot per year, are concentrated near the flood-plain margin along tributary streams. In conjunction with beaver dams and debris, ongoing sedimentation has blocked flow in several tributaries, posing a flood hazard to agricultural land near the flood-plain margin. The occluded tributaries likely will continue to overflow unless they are periodically dredged or their sediment loads are reduced.

  15. Different seasonality of nitrate export from an agricultural watershed and an urbanized watershed in Midwestern USA

    NASA Astrophysics Data System (ADS)

    Tian, S.; Youssef, M. A.; Richards, R. P.; Liu, J.; Baker, D. B.; Liu, Y.

    2016-10-01

    Land use/land cover is a critical factor affecting temporal dynamics of nitrate export from watersheds. Based on a long-term (>30 years) water quality monitoring program in the Western Lake Erie area, United States, this study compared seasonal variation of nitrate export from an agricultural watershed and an urbanized watershed. A seasonality index was adapted to quantitatively characterize seasonal variation of nitrate export from the two watersheds. Results showed that monthly nitrate concentrations from the two watersheds exhibited different seasonal variation. Seasonality index of monthly nitrate loading for the agricultural watershed is approximately 3 times of that from the urbanized watershed and the difference is statistically significant (p < 0.0001). Meanwhile, calculated historical seasonality indexes of monthly nitrate loading for both watersheds exhibited significant (p < 0.05) decreasing trends according to the non-seasonal Mann-Kendall test. The identified differences in seasonal nitrate export from the two watersheds were mainly attributed to their distinct nitrogen sources, physical and biogeochemical settings. The declining seasonality index of monthly nitrate loading from the agricultural watershed could be partially caused by historical climate change in the study region, especially increased temperature during winter. Urbanization could be one key factor contributing to the declining seasonality index of monthly nitrate loading from the urbanized watershed. Information derived from this study have practical implications for developing proper management practices to mitigate nitrate pollution in Midwestern United States.

  16. Geochemical baseline studies and relations between water quality and streamflow in the upper Blackfoot Watershed, Montana: data for July 1997-December 1998

    USGS Publications Warehouse

    Nagorski, Sonia A.; Moore, Johnnie N.; Smith, David B.

    2001-01-01

    We used ultraclean sampling techniques to study the solute (operationally defined as <0.2 ?m) surface water geochemistry at five sites along the Upper Blackfoot River and four sites along the Landers Fork, some in more detail and more regularly than others. We collected samples also from Hogum Creek, a tributary to the Blackfoot, from Copper Creek, a tributary to the Landers Fork, and from ground water seeps contributing to the flow along the Landers Fork. To better define the physical dynamics of the hydrologic system and to determine geochemical loads, we measured streamflow at all the sites where we took samples for water quality analysis. The Upper Blackfoot River, which drains historic mines ca. 20 Km upstream of the study area, had higher trace metal concentrations than did the Landers Fork, which drains the pristine Scapegoat Wilderness area. In both rivers, many of the major elements were inversely related to streamflow, and at some sites, several show a hysteresis effect in which the concentrations were lower on the rising limb of the hydrograph than on the falling limb. However, many of the trace elements followed far more irregular trends, especially in the Blackfoot River. Elements such as As, Cu, Fe, Mn, S, and Zn exhibited complex and variable temporal patterns, which included almost no response to streamflow differences, increased concentrations following a summer storm and at the start of snowmelt in the spring, and/or increased concentrations throughout the course of spring runoff. In summary, complex interactions between the timing and magnitude of streamflow with physical and chemical processes within the watershed appeared to greatly influence the geochemistry at the sites, and streamflow values alone were not good predictors of solute concentrations in the rivers.

  17. Mercury Loads in the South River and Simulation of Mercury Total Maximum Daily Loads (TMDLs) for the South River, South Fork Shenandoah River, and Shenandoah River: Shenandoah Valley, Virginia

    USGS Publications Warehouse

    Eggleston, Jack

    2009-01-01

    Due to elevated levels of methylmercury in fish, three streams in the Shenandoah Valley of Virginia have been placed on the State's 303d list of contaminated waters. These streams, the South River, the South Fork Shenandoah River, and parts of the Shenandoah River, are downstream from the city of Waynesboro, where mercury waste was discharged from 1929-1950 at an industrial site. To evaluate mercury contamination in fish, this total maximum daily load (TMDL) study was performed in a cooperative effort between the U.S. Geological Survey, the Virginia Department of Environmental Quality, and the U.S. Environmental Protection Agency. The investigation focused on the South River watershed, a headwater of the South Fork Shenandoah River, and extrapolated findings to the other affected downstream rivers. A numerical model of the watershed, based on Hydrological Simulation Program-FORTRAN (HSPF) software, was developed to simulate flows of water, sediment, and total mercury. Results from the investigation and numerical model indicate that contaminated flood-plain soils along the riverbank are the largest source of mercury to the river. Mercury associated with sediment accounts for 96 percent of the annual downstream mercury load (181 of 189 kilograms per year) at the mouth of the South River. Atmospherically deposited mercury contributes a smaller load (less than 1 percent) as do point sources, including current discharge from the historic industrial source area. In order to determine how reductions of mercury loading to the stream could reduce methylmercury concentrations in fish tissue below the U.S. Environmental Protection Agency criterion of 0.3 milligrams per kilogram, multiple scenarios were simulated. Bioaccumulation of mercury was expressed with a site-specific exponential relation between aqueous total mercury and methylmercury in smallmouth bass, the indicator fish species. Simulations indicate that if mercury loading were to decrease by 98.9 percent from 189

  18. Elk River Watershed - Flood Study

    NASA Astrophysics Data System (ADS)

    Barnes, C. C.; Byrne, J. M.; MacDonald, R. J.; Lewis, D.

    2014-12-01

    Flooding has the potential to cause significant impacts to economic activities as well as to disrupt or displace populations. Changing climate regimes such as extreme precipitation events increase flood vulnerability and put additional stresses on infrastructure. Potential flooding from just under 100 (2009 NPRI Reviewed Facility Data Release, Environment Canada) toxic tailings ponds located in Canada increase risk to human safety and the environment. One such geotechnical failure spilt billions of litres of toxic tailings into the Fraser River watershed, British Columbia, when a tailings pond dam breach occurred in August 2014. Damaged and washed out roadways cut access to essential services as seen by the extensive floods that occurred in Saskatchewan and Manitoba in July 2014, and in Southern Alberta in 2013. Recovery efforts from events such as these can be lengthy, and have substantial social and economic impacts both in loss of revenue and cost of repair. The objective of this study is to investigate existing conditions in the Elk River watershed and model potential future hydrological changes that can increase flood risk hazards. By analyzing existing hydrology, meteorology, land cover, land use, economic, and settlement patterns a baseline is established for existing conditions in the Elk River watershed. Coupling the Generate Earth Systems Science (GENESYS) high-resolution spatial hydrometeorological model with flood hazard analysis methodology, high-resolution flood vulnerability base line maps are created using historical climate conditions. Further work in 2015 will examine possible impacts for a range of climate change and land use change scenarios to define changes to future flood risk and vulnerability.

  19. ANALYZING CORRELATIONS BETWEEN STREAM AND WATERSHED ATTRIBUTES

    EPA Science Inventory

    Bivariate correlation analysis has been widely used to explore relationships between stream and watershed attributes that have all been measured on the same set of watersheds or sampling locations. Researchers routinely test H0: =0 for each correlation in a large table and then ...

  20. Upper Washita River experimental watersheds: Sediment Database

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Improving the scientific understanding of the effectiveness of watershed conservation practices and floodwater-retardation structures to control floods and soil erosion is one of the primary objectives for sediment studies in the upper Washita River Experimental Watersheds. This paper summarizes se...

  1. WATERSHED LANDSCAPE INDICATORS OF ESTUARINE BENTHIC CONDITION

    EPA Science Inventory

    Do land use/cover characteristics of watersheds associated with small estuaries exhibit a strong enough signal to make landscape metrics useful for identifying degraded bottom communities? We tested this idea with 58 pairs of small estuaries (<260 km2) and watersheds in the U.S. ...

  2. Watershed: A Successful Voyage into Integrative Learning.

    ERIC Educational Resources Information Center

    Springer, Mark

    This book describes a "whole learning" approach to education called the Watershed Program, which stresses integrated curriculum and experiential learning. Each chapter begins with an episode from the history of eastern Pennsylvania along the Brandywine River, used as an analogy to problems faced by the teachers in the Watershed program.…

  3. Healthy Watersheds Integrated Assessments Workshop Synthesis

    EPA Science Inventory

    The U.S. Environmental Protection Agency, in partnership with others, is embarking on the new Healthy Watersheds Initiative to protect our remaining healthy watersheds, prevent them from becoming impaired, and accelerate our restoration successes. In November 2010, a Healthy Wate...

  4. Draft Maumee River Watershed Restoration Plan

    EPA Pesticide Factsheets

    A draft of the Maumee River AOC Watershed Restoration Plan was completed in January 2006. The plan was created to meet requirements for the stage II RAP as well as Ohio EPA’s and ODNR’s Watershed Coordinator Program.

  5. Watershed Deposition Tool for air quality impacts

    EPA Pesticide Factsheets

    The WDT is a software tool for mapping deposition estimates from the CMAQ model to watersheds. It provides users with the linkage of air and water needed for the total maximum daily load (TMDL) and related nonpoint-source watershed analyses.

  6. Watershed Scale Impacts of Stormwater Green Infrastructure ...

    EPA Pesticide Factsheets

    Despite the increasing use of urban stormwater green infrastructure (SGI), including detention ponds and rain gardens, few studies have quantified the cumulative effects of multiple SGI projects on hydrology and water quality at the watershed scale. To assess the effects of SGI, Baltimore County, MD, Montgomery County, MD, and Washington, DC, were selected based on the availability of data on SGI, water quality, and stream flow. The watershed scale impact of SGI was evaluated by assessing how increased spatial density of SGI correlates with stream hydrology and nitrogen exports over space and time. The most common SGI types were detention ponds (58%), followed by marshes (12%), sand filters (9%), wet ponds (7%), infiltration trenches (4%), and rain gardens (2%). When controlling for watersheds size and percent impervious surface cover, watersheds with greater amounts of SGI (>10% SGI) have 44% lower peak runoff, 26% less frequent runoff events, and 26% less variable runoff than watersheds with lower SGI. Watersheds with more SGI also show 44% less NO3− and 48% less total nitrogen exports compared to watersheds with minimal SGI. There was no significant reduction in combined sewer overflows in watersheds with greater SGI. Based on specific SGI types, infiltration trenches (R2 = 0.35) showed the strongest correlation with hydrologic metrics, likely due to their ability to attenuate flow, while bioretention (R2 = 0.19) and wet ponds (R2 = 0.12) showed stronger

  7. Uncertainty Consideration in Watershed Scale Models

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Watershed scale hydrologic and water quality models have been used with increasing frequency to devise alternative pollution control strategies. With recent reenactment of the 1972 Clean Water Act’s TMDL (total maximum daily load) component, some of the watershed scale models are being recommended ...

  8. Role of adaptive management for watershed councils.

    PubMed

    Habron, Geoffrey

    2003-01-01

    Recent findings in the Umpqua River Basin in southwestern Oregon illustrate a tension in the rise of both community-based and watershed-based approaches to aquatic resource management. While community-based institutions such as watershed councils offer relief from the government control landowners dislike, community-based approaches impinge on landowners' strong belief in independence and private property rights. Watershed councils do offer the local control landowners advocate; however, institutional success hinges on watershed councils' ability to reduce bureaucracy, foster productive discussion and understanding among stakeholders, and provide financial, technical, and coordination support. Yet, to accomplish these tasks current watershed councils rely on the fiscal and technical capital of the very governmental entities that landowners distrust. Adaptive management provides a basis for addressing the apparent tension by incorporating landowners' belief in environmental resilience and acceptance of experimentation that rejects "one size fits all solutions." Therefore community-based adaptive watershed management provides watershed councils a framework that balances landowners' independence and fear of government intrusion, acknowledges the benefits of community cooperation through watershed councils, and enables ecological assessment of landowner-preferred practices. Community-based adaptive management integrates social and ecological suitability to achieve conservation outcomes by providing landowners the flexibility to use a diverse set of conservation practices to achieve desired ecological outcomes, instead of imposing regulations or specific practices.

  9. Watershed modeling applications in south Texas

    USGS Publications Warehouse

    Pedraza, Diana E.; Ockerman, Darwin J.

    2012-01-01

    This fact sheet presents an overview of six selected watershed modeling studies by the USGS and partners that address a variety of water-resource issues in south Texas. These studies provide examples of modeling applications and demonstrate the usefulness and versatility of watershed models in aiding the understanding of hydrologic systems.

  10. Hg Isotopes Reveal Importance of In-Stream Processing and Legacy Inputs in East Fork Poplar Creek, Oak Ridge, Tennessee, USA.

    NASA Astrophysics Data System (ADS)

    Demers, J. D.; Blum, J. D.; Brooks, S. C.; Donovan, P. M.; Gu, B.; Riscassi, A.

    2015-12-01

    Understanding how mercury (Hg) contaminated ecosystems will recover as atmospheric emissions and industrial point source discharges are controlled has become a driving motivation of mercury research. Key to predicting recovery of mercury contaminated ecosystems is an understanding of the mobilization of legacy Hg sources, and the subsequent bioavailability and biogeochemical cycling of mobilized Hg within aquatic ecosystems. Herein, we utilize natural abundance stable Hg isotope techniques to place new constraints on mercury sources, transport, and transformations along the flow path of East Fork Poplar Creek (EFPC), Oak Ridge, Tennessee. The isotopic composition of mercury in stream water and suspended sediment along the flow path suggest that: (1) physical processes such as dilution and sedimentation cannot fully explain decreases in total mercury concentrations along the flow path and that in-stream processes may be more important than previously realized; (2) in-stream processes include photochemical transformations (~20%), but microbial reduction is likely more dominant (~80%); and (3) additional sources of mercury inputs to EFPC at base-flow may predominantly arise from the hyporheic zone during the growing season, with adjacent riparian wetlands and non-point-source impacted tributaries increasing in importance during the dormant season when the stream channel is more hydrologically connected to the watershed.

  11. Measuring environmental sustainability of water in watersheds.

    PubMed

    Hester, Erich T; Little, John C

    2013-08-06

    Environmental sustainability assessment is a rapidly growing field where measures of sustainability are used within an assessment framework to evaluate and compare alternative actions. Here we argue for the importance of evaluating environmental sustainability of water at the watershed scale. We review existing frameworks in brief before reviewing watershed-relevant measures in more detail. While existing measures are diverse, overlapping, and interdependent, certain attributes that are important for watersheds are poorly represented, including spatial explicitness and the effect of natural watershed components, such as rivers. Most studies focus on one or a few measures, but a complete assessment will require use of many existing measures, as well as, perhaps, new ones. Increased awareness of the broad dimensions of environmental sustainability as applied to water management should encourage integration of existing approaches into a unified assessment framework appropriate for watersheds.

  12. Protecting water quality in the watershed

    SciTech Connect

    James, C.R.; Johnson, K.E. ); Stewart, E.H. )

    1994-08-01

    This article highlights the water quality component of a watershed management plan being developed for the San Francisco (CA) Water Department. The physical characteristics of the 63,000-acre watersheds were analyzed for source and transport vulnerability for five groups of water quality parameters--particulates, THM precursors, microorganisms (Giardia and cryptosporidium), nutrients (nitrogen and phosphorus), and synthetic organic chemicals--and vulnerability zones were mapped. Mapping was achieved through the use of an extensive geographic information system (GIS) database. Each water quality vulnerability zone map was developed based on five watershed physical characteristics--soils, slope, vegetation, wildlife concentration, and proximity to water bodies--and their relationships to each of the five groups of water quality parameters. An approach to incorporate the watershed physical characteristics information into the five water quality vulnerability zone maps was defined and verified. The composite approach was based in part on information gathered from existing watershed management plans.

  13. Storm water control plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    SciTech Connect

    1996-04-01

    This document provides the Environmental Restoration Program with information about the erosion and sediment control, storm water management, maintenance, and reporting and record keeping practices to be employed during Phase II of the remediation project for the Lower East Fork Poplar Creek (LEFPC) Operable Unit.

  14. 75 FR 25197 - Shasta Trinity National Forest, South Fork Management Unit, California Salt Timber Harvest and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ... Forest Service Shasta Trinity National Forest, South Fork Management Unit, California Salt Timber Harvest... statement for the Salt Timber Harvest and Fuels Reduction Project (Salt Project). A supplemental environmental impact statement will be prepared for the Salt Project to supplement wildlife management...

  15. Numerical performance analysis of quartz tuning fork-based force sensors

    NASA Astrophysics Data System (ADS)

    Dagdeviren, Omur E.; Schwarz, Udo D.

    2017-01-01

    Quartz tuning fork-based force sensors where one prong is immobilized onto a holder while the other one is allowed to oscillate freely (‘qPlus’ configuration) are in widespread use for high-resolution scanning probe microscopy applications. Due to the small size of the tuning forks (≈3 mm) and the complexity of the sensor assemblies, the reliable and repeatable manufacturing of the sensors has been challenging. In this paper, we investigate the contribution of the amount and location of the epoxy glue used to attach the tuning fork to its holder on the sensor’s performance. Towards this end, we use finite element analysis to model the entire sensor assembly and to perform static and dynamic numerical simulations. Our analysis reveals that increasing the thickness of the epoxy layer between prong and holder results in a decrease of the sensor’s spring constant, eigenfrequency, and quality factor while showing an increasing deviation from oscillation in its primary modal shape. Adding epoxy at the sides of the tuning fork also leads to a degradation of the quality factor even though in this case, spring constant and eigenfrequency rise in tandem with a lessening of the deviation from its ideal modal shape.

  16. Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

    PubMed Central

    Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; Juhasz, Szilvia; Morocz, Monika; Gali, Himabindu; Dhe-Paganon, Sirano; Venclovas, Česlovas; Haracska, Lajos

    2015-01-01

    Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteins retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. In more general terms, we suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal. PMID:26350214

  17. Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks.

    PubMed

    Sotiriou, Sotirios K; Kamileri, Irene; Lugli, Natalia; Evangelou, Konstantinos; Da-Ré, Caterina; Huber, Florian; Padayachy, Laura; Tardy, Sebastien; Nicati, Noemie L; Barriot, Samia; Ochs, Fena; Lukas, Claudia; Lukas, Jiri; Gorgoulis, Vassilis G; Scapozza, Leonardo; Halazonetis, Thanos D

    2016-12-15

    Human cancers are characterized by the presence of oncogene-induced DNA replication stress (DRS), making them dependent on repair pathways such as break-induced replication (BIR) for damaged DNA replication forks. To better understand BIR, we performed a targeted siRNA screen for genes whose depletion inhibited G1 to S phase progression when oncogenic cyclin E was overexpressed. RAD52, a gene dispensable for normal development in mice, was among the top hits. In cells in which fork collapse was induced by oncogenes or chemicals, the Rad52 protein localized to DRS foci. Depletion of Rad52 by siRNA or knockout of the gene by CRISPR/Cas9 compromised restart of collapsed forks and led to DNA damage in cells experiencing DRS. Furthermore, in cancer-prone, heterozygous APC mutant mice, homozygous deletion of the Rad52 gene suppressed tumor growth and prolonged lifespan. We therefore propose that mammalian RAD52 facilitates repair of collapsed DNA replication forks in cancer cells.

  18. 76 FR 46721 - Salmon-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... County and the State of Idaho. Lemhi County's Wildfire Prevention Plan has designated the North Fork... the potential for large-scale, high-intensity wildfires that threaten human life, property, and... uncharacteristic high-intensity wildfires and landscape restoration needs in the project. It is important...

  19. 78 FR 74005 - Amendment of Class D and Class E Airspace; Grand Forks, ND

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ... MSL within a 4.2-mile radius of Grand Forks International Airport. This Class D airspace area is... airspace extending upward from the surface to and including 3,400 feet MSL within a 4.9-mile radius of....9-mile radius to 5.6 miles south of the airport, excluding that airspace within the Grand...

  20. A chloroplast DNA helicase II from pea that prefers fork-like replication structures

    PubMed

    Tuteja; Phan

    1998-11-01

    A DNA helicase, called chloroplast DNA (ctDNA) helicase II, was purified to apparent homogeneity from pea (Pisum sativum). The enzyme contained intrinsic, single-stranded, DNA-dependent ATPase activity and an apparent molecular mass of 78 kD on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The DNA helicase was markedly stimulated by DNA substrates with fork-like replication structures. A 5'-tailed fork was more active than the 3'-tailed fork, which itself was more active than substrates without a fork. The direction of unwinding was 3' to 5' along the bound strand, and it failed to unwind blunt-ended duplex DNA. DNA helicase activity required only ATP or dATP hydrolysis. The enzyme also required a divalent cation (Mg2+>Mn2+>Ca2+) for its unwinding activity and was inhibited at 200 mM KCl or NaCl. This enzyme could be involved in the replication of ctDNA. The DNA major groove-intercalating ligands nogalamycin and daunorubicin were inhibitory to unwinding (Ki approximately 0.85 &mgr;M and 2.2 &mgr;M, respectively) and ATPase (Ki approximately 1.3 &mgr;M and 3.0 &mgr;M, respectively) activities of pea ctDNA helicase II, whereas ellipticine, etoposide (VP-16), and camptothecin had no effect on the enzyme activity. These ligands may be useful in further studies of the mechanisms of chloroplast helicase activities.