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Sample records for fork projects thompson

  1. Wildlife Impact Assessment and Summary of Previous Mitigation Related to Hydroelectric Projects in Montana, Phase I, Volume Two (A), Clark Fork Projects, Thompson Falls Dam, Operator, Montana Power Company.

    SciTech Connect

    Wood, Marilyn

    1984-03-27

    The Thompson Falls Dam inundated approximately 347 acres of wildlife habitat that likely included conifer forests, deciduous bottoms, mixed conifer-deciduous forests and grassland/hay meadows. Additionally, at least one island, and several gravel bars were inundated when the river was transformed into a reservoir. The loss of riparian and riverine habitat adversely affected the diverse wildlife community inhabiting the lower Clark Fork River area. Quantitative loss estimates were determined for selected target species based on best available information. The loss estimates were based on inundation of the habitat capable of supporting the target species. Whenever possible, loss estimates bounds were developed by determining ranges of impacts based on density estimates and/or acreage loss estimates. Of the twelve target species or species groups, nine were assessed as having net negative impacts. 86 refs., 2 figs., 5 tabs.

  2. Final 2014 Remedial Action Report Project Chariot, Cape Thompson, Alaska

    SciTech Connect

    None, None

    2015-03-01

    This report was prepared to document remedial action (RA) work performed at the former Project Chariot site located near Cape Thompson, Alaska during 2014. The work was managed by the U.S. Army Corps of Engineers (USACE) Alaska District for the U.S. Department of Energy (DOE) Office of Legacy Management (LM). Due to the short field season and the tight barge schedule, all field work was conducted at the site July 6 through September 12, 2014. Excavation activities occurred between July 16 and August 26, 2014. A temporary field camp was constructed at the site prior to excavation activities to accommodate the workers at the remote, uninhabited location. A total of 785.6 tons of petroleum, oil, and lubricants (POL)-contaminated soil was excavated from four former drill sites associated with test holes installed circa 1960. Diesel was used in the drilling process during test hole installations and resulted in impacts to surface and subsurface soils at four of the five sites (no contamination was identified at Test Hole Able). Historic information is not definitive as to the usage for Test Hole X-1; it may have actually been a dump site and not a drill site. In addition to the contaminated soil, the steel test hole casings were decommissioned and associated debris was removed as part of the remedial effort.

  3. 77 FR 45597 - Middle Fork American River Project; Notice of Availability of the Draft Environmental Impact...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-01

    ... Energy Regulatory Commission Middle Fork American River Project; Notice of Availability of the Draft Environmental Impact Statement for the Middle Fork American River Hydrolectric Project and Intention To Hold... Fork American River Hydroelectric Project (FERC No. 2079), located on the Middle Fork of the...

  4. 78 FR 13660 - Middle Fork American River Project; Notice of Availability of the Final Environmental Impact...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Energy Regulatory Commission Middle Fork American River Project; Notice of Availability of the Final Environmental Impact Statement for the Middle Fork American River Hydrolectric Project In accordance with the... Projects has reviewed the application for license for the Middle Fork American River Hydroelectric...

  5. Oak Grove Fork Habitat Improvement Project, 1988 Annual Report.

    SciTech Connect

    Bettin, Scott

    1989-04-01

    The Lower Oak Grove Fork of the Clackamas River is a fifth-order tributary of the Clackamas River drainage supporting depressed runs of coho and chinook salmon, and summer and winter steelhead. Habitat condition rating for the Lower Oak Grove is good, but smelt production estimates are below the average for Clackamas River tributaries. Limiting factors in the 3.8 miles of the Lower Oak Grove supporting anadromous fish include an overall lack of quality spawning and rearing habitat. Beginning in 1986. measures to improve fish habitat in the Lower Oak Grove were developed in coordination with the Oregon Department of Fish and Wildlife (ODF&W) and Portland General Electric (PGE) fisheries biologists. Prior to 1986, no measures had been applied to the stream to mitigate for PGE's storage and regulation of flows in the Oak Grove Fork (Timothy Lake, Harriet Lake). Catchable rainbow trout are stocked by ODF&W two or three times a year during the trout fishing season in the lowermost portion of the Oak Grove Fork near two Forest Service campgrounds (Ripplebrook and Rainbow). The 1987 field season marked the third year of efforts to improve fish habitat of the Lower Oak Grove Fork and restore anadromous fish production. The efforts included the development of an implementation plan for habitat improvement activities in the Lower Oak Grove Fork. post-project monitoring. and maintenance of the 1986 improvement structures. No new structures were constructed or placed in 1987. Fiscal year 1988 brought a multitude of changes which delayed implementation of plans developed in 1987. The most prominent change was the withdrawal of the proposed Spotted Owl Habitat Area (SOHA) which overlapped the Oak Grove project implementation area. Another was the change in the Forest Service biologist responsible for implementation and design of this project.

  6. 77 FR 47058 - Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Middle Fork American River Hydroelectric Project Placer County Water Agency... comments on the draft environmental impact statement for the Middle Fork American River Project No....

  7. 77 FR 55796 - Sand Lick Fork Watershed Restoration Project; Daniel Boone National Forest, KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ... Forest Service Sand Lick Fork Watershed Restoration Project; Daniel Boone National Forest, KY AGENCY... Sand Lick Fork Watershed Restoration Project involves activities to improve water quality and reduce soil loss by plugging abandoned oil wells, removing abandoned flow lines, restoration of...

  8. South Fork Snake River/Palisades Wildlife Mitigation Project: Environmental assessment

    SciTech Connect

    1995-09-01

    BPA proposes to fund the implementation of the South Fork Snake River Programmatic Management Plan to compensate for losses of wildlife and wildlife habitat due to hydroelectric development at Palisades Dam. The Idaho Department of Fish and Game drafted the plan, which was completed in May 1993. This plan recommends land and conservation easement acquisition and wildlife habitat enhancement measures. These measures would be implemented on selected lands along the South Fork of the Snake River between Palisades Dam and the confluence with the Henry`s Fork, and on portions of the Henry`s Fork located in Bonneville, Madison, and Jefferson Counties, Idaho. BPA has prepared an Environmental Assessment evaluating the proposed project. The EA also incorporates by reference the analyses in the South Fork Snake River Activity/Operations Plan and EA prepared jointly in 1991 by the Bureau of Land Management and the Forest Service. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an Environmental Impact Statement (EIS) is not required and BPA is issuing this FONSI.

  9. Project Management Done Here: Lois Langer--Thompson Hennepin County Library, MN

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    How do you simultaneously renovate a grocery store into a state-of-the-art resource library, supervise the daily operations of five libraries, lead a team in reimagining reference service, and plan your wedding? Ask Lois Langer, coordinating librarian of Hennepin County Library. Her answer: "Project management--you can do anything, professional…

  10. 3. SOUTH FORK OF THE TULE RIVER MIDDLE FORK BRANCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. SOUTH FORK OF THE TULE RIVER MIDDLE FORK BRANCH FLUME AT THE NORTH FORK OF THE TULE RIVER MIDDLE FORK CROSSING SHOWING ORIGINAL DIMENSIONAL STONE PIER ON WEST BANK AT PHOTO CENTER, AND REMAINS OF ORIGINAL EAST BANK DIMENSIONAL STONE PIER AT PHOTO LEFT BELOW NEW (ca. 1931) EAST BANK PIER. VIEW TO SOUTHEAST. - Tule River Hydroelectric Project, Water Conveyance System, Middle Fork Tule River, Springville, Tulare County, CA

  11. The Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A.; Melsheimer, T.; Rideout, C.; Vanlew, K.

    1998-12-01

    The Little Thompson Observatory is believed to be the first observatory built as part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction is nearly completed and first light is planned for fall 1998. The observatory is located on the grounds of Berthoud High School in northern Colorado. Local schools and youth organizations will have prioritized access to the telescope, and there will also be opportunities for public viewing. After midnight, the telescope will be open to world-wide use by schools via the Internet following the model of the first TIE observatory, the 24" telescope on Mt. Wilson. That telescope has been in use for the past four years by up to 50 schools per month. Students remotely connect to the observatory over the Internet, and then receive the images on their local computers. The observatory grew out of grassroots support from the local community surrounding Berthoud, Colorado, a town of 3,500 residents. TIE has provided the observatory with a Tinsley 18" Cassegrain telescope on a 10-year loan. The facility has been built with tremendous support from volunteers and the local school district. We have applied for an IDEAS grant to provide teacher training workshops which will allow K-12 schools in northern Colorado to make use of the Little Thompson Observatory, including remote observing from classrooms.

  12. The Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A.; Melsheimer, T.; Sackett, C.

    1999-05-01

    The Little Thompson Observatory is believed to be the first observatory built as part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building and dome has been completed, and first light is planned for spring 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. Local schools and youth organizations will have prioritized access to the telescope, and there will also be opportunities for public viewing. After midnight, the telescope will be open to world-wide use by schools via the Internet following the model of the first TIE observatory, the 24" telescope on Mt. Wilson. Students remotely connect to the observatory over the Internet, and then receive the images on their local computers. The observatory grew out of grassroots support from the local community surrounding Berthoud, Colorado, a town of 3,500 residents. TIE has provided the observatory with a Tinsley 18" Cassegrain telescope on a 10-year loan. The facility has been built with tremendous support from volunteers and the local school district. We have received an IDEAS grant to provide teacher training workshops which will allow K-12 schools in northern Colorado to make use of the Little Thompson Observatory, including remote observing from classrooms.

  13. The Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; VanLew, K.; Melsheimer, T.; Sackett, C.

    1999-12-01

    The Little Thompson Observatory is the second member of the Telescopes in Education (TIE) project. Construction of the dome and the remote control system has been completed, and the telescope is now on-line and operational over the Internet. The observatory is located on the grounds of Berthoud High School in northern Colorado. Local schools and youth organizations have prioritized access to the telescope, and there are monthly opportunities for public viewing. In the future, the telescope will be open after midnight to world-wide use by schools following the model of the first TIE observatory, the 24" telescope on Mt. Wilson. Students remotely connect to the observatory over the Internet, and then receive the images on their local computers. The observatory grew out of grassroots support from the local community surrounding Berthoud, Colorado, a town of 3,500 residents. TIE has provided the observatory with a Tinsley 18" Cassegrain telescope on a 10-year loan. The facility has been built with tremendous support from volunteers and the local school district. With funding from an IDEAS grant, we have begun teacher training workshops which will allow K-12 schools in northern Colorado to make use of the Little Thompson Observatory, including remote observing from classrooms.

  14. Milton (Milt) O. Thompson

    NASA Technical Reports Server (NTRS)

    1960-01-01

    Milton O. Thompson was hired as an aeronautical research scientist at the National Advisory Committee for Aeronautics' High-Speed Flight Station, Edwards, California, on March 19, 1956, becoming a research pilot in January 1958. During his 37-year career at what became the National Aeronautics and Space Administration's Dryden Flight Research Center in 1976, Milt managed several research programs and flew many research flights. One of Milt's first research projects, after he became a research pilot, was a program to investigate the feasibility of obtaining substantial amounts of laminar flow on an airfoil at supersonic speeds. The testbed aircraft was an F-104 with one wing covered with a fiberglass glove that served as the test section for the experiment. Next was the Air Launched Sounding Rocket (ALSOR) research program using an F-104 with a rocket launcher installed on it. The intent of the program was to release a balloon from an air launched rocket at over 1,000,000 feet altitude (approximately 190 miles) and then measure its rate of descent to determine air density. In 1959, Thompson was assigned to the Boeing X-20 Dyna-Soar program as a pilot-consultant. The X-20 program was scheduled to launch a human into Earth orbit and recover with a horizontal ground landing. The program was canceled before construction of the vehicle began. Milt became interested in the Rogallo-wing concept, 'Parawing', for spacecraft reentry. The best way to acquire experience, of course, was by building and flying a Paraglider Research Vehicle (Paresev). After ground tows to demonstrate controllability with Milt in the cockpit, he made the first flight aloft on March 12, 1962. On August 16, 1963 Thompson became the first person to fly a lifting body, the lightweight M2-F1. The plywood and steel-tubing prototype was flown as a glider after releasing from an R4D tow plane. He flew it a total of 47 times, and also made the first five flights of the all-metal M2-F2 lifting body

  15. 1. NORTH FORK OF THE TULE RIVER MIDDLE FORK BRANCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. NORTH FORK OF THE TULE RIVER MIDDLE FORK BRANCH FLUME AND CONCRETE DIVERSION DAM SPILLING WATER. CONCRETE ABUTMENTS OF THE ORIGINAL HIGHWAY 190 BRIDGE OVER THE NORTH FORK ARE VISIBLE ON EITHER SIDE OF THE DAM. NEW HIGHWAY 190 BRIDGE IS VISIBLE ACROSS TOP OF PHOTO. VIEW TO NORTH. - Tule River Hydroelectric Project, Water Conveyance System, Middle Fork Tule River, Springville, Tulare County, CA

  16. Interview with Sandra Thompson.

    ERIC Educational Resources Information Center

    Huang, Chiung-chih

    1994-01-01

    Presents an interview of Sandra Thompson on various topics relating to the Chinese language. The interview touches on conversational data on Chinese, the lack of morphological complexity in Mandarin Chinese, and the development of Chinese functionalism. (12 references) (CK)

  17. North Fork John Day Dredge Tailings Restoration Project Final Report 1997-2002.

    SciTech Connect

    Sanchez, John A.

    2002-12-01

    The USDA Forest Service (USFS) and the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) worked together to rehabilitate 2.1 miles of Clear Creek floodplain, a tributary of the North Fork John Day River Basin. Dredge tailing were deposited from mining operations on Clear Creek's floodplain from the 1930's to the 1950's. These tailing confined the stream channel and degraded the floodplain. The work was completed by moving dredge tailing piles adjacent to the Clear Creek channel, using track-mounted excavators and dump trucks. A caterpillar tractor was used to contour the material placed outside the immediate floodplain, blending it into the hillside. The restored floodplain was very near channel bankfull level following excavation and contoured to accept future flood flows. Monitoring was initiated through pre and post-project photo points and cross-section measurements. Work was completed in two efforts. In 1997 and 1998 floodplain restoration was adjacent to the reconstruction of Road 13 from the junction with Road 10 from Clear Creek River Mile 1.9 to 3.1 for a distance of 1.2 miles. In 1999 the Environmental Assessment for Lower Clear Creek--Granite Creek Floodplain Restoration Project was completed for work proposed on Clear Creek from the mouth up to River mile 1.9 and the Granite Creek floodplain from River miles 5.9 to 7.7. Restoration proposed in the 1999 Environmental Assessment is the subject of this report.

  18. Waste Management Plan for the Lower East Fork Poplar Creek Remedial Action Project Oak Ridge, Tennessee

    SciTech Connect

    1996-08-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Landfill V, and restore the affected floodplain upon completion of remediation activities. This effort will be conducted in accordance with the Record of Decision (ROD) for LEFPC as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) action. The Waste Management Plan addresses management and disposition of all wastes generated during the remedial action for the LEFPC Project Most of the solid wastes will be considered to be sanitary or construction/demolition wastes and will be disposed of at existing Y-12 facilities for those types of waste. Some small amounts of hazardous waste are anticipated, and the possibility of low- level or mixed waste exists (greater than 35 pCi/g), although these are not expected. Liquid wastes will be generated which will be sanitary in nature and which will be capable of being disposed 0214 of at the Oak Ridge Sewage Treatment Plant.

  19. South Fork Tolt River Hydroelectric Project : Adopted Portions of a 1987 Federal Energy Regulatory Commission`s Final Environmental Impact Statement.

    SciTech Connect

    United States. Bonneville Power Administration.

    1992-07-01

    The South Fork Tolt River Hydroelectric Project that world produce 6.55 average megawatts of firm energy per year and would be sited in the Snohomish River Basin, Washington, was evaluated by the Federal Energy Regulatory commission (FERC) along with six other proposed projects for environmental effects and economic feasibility Based on its economic analysis and environmental evaluation of the project, the FERC staff found that the South Fork Tolt River Project would be economically feasible and would result in insignificant Impacts if sedimentation issues could be resolved. Upon review, the BPA is adopting portions of the 1987 FERC FEIS that concern the South Fork Tolt River Hydroelectric Project and updating specific sections in an Attachment.

  20. Wildlife and Wildlife Habitat Loss Assessment at Dexter Dam and Reservoir Project, Middle Fork Willamette River, Oregon, 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Dexter Dam and Reservoir Project on the Middle Fork Willamette River, Oregon, to determine losses or gains resulting from the development and operation of the project. Preconstruction, post-construction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1944, 1956, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Fifteen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Dexter Project extensively altered or affected 4662 acres of land and river in the Middle Fork Willamette River drainage. Impacts to wildlife centered around the loss of 445 acres of riparian habitat. Impacts resulting from the Dexter Project included the loss of year-round habitat for black-tailed deer, red fox, mink, beaver, western gray squirrel, ruffed grouse, ring-necked pheasant, California quail, wood duck and nongame species. Bald eagle, osprey, and greater scaup were benefitted by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Dexter Project. Losses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  1. Arsenic Removal from Drinking Water by Coagulation/Filtration U.S. EPA Demonstration Project at City of Three Forks, MT, Final Performance Evaluation Report

    EPA Science Inventory

    This report documents the activities performed during and the results obtained from the arsenic removal treatment technology demonstration project at the City of Three Forks, MT facility. The objectives of the project were to evaluate: 1) the effectiveness of Kinetico’s FM-248-A...

  2. Best management practices plan for the Lower East Fork Poplar Creek remedial action project, Oak Ridge, Tennessee

    SciTech Connect

    1996-08-01

    The U.S. Department of Energy (DOE) has three major operating facilities on the DOE Oak Ridge Reservation (ORR) in Oak Ridge, Tennessee: the Oak Ridge Y-12 Plant, the K-25 Site, and the Oak Ridge National Laboratory (ORNL) managed by Lockheed Martin Environmental Research Corporation. All facilities are managed by Lockheed Martin Energy Systems, Incorporated (Energy Systems) for the DOE. The Y-12 Plant is adjacent to the city of Oak Ridge and is also upstream from Oak Ridge along East Fork Poplar Creek. The portion of the creek downstream from the Y-12 Plant is Lower East Fork Poplar Creek (LEFPC). This project will remove mercury-contaminated soils from the LEFPC floodplain, transport the soils to Industrial Landfill V (ILF-V), and restore any affected areas. This project contains areas that were designated in 1989 as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) site. The site includes DOE property and portions of commercial, residential, agricultural, and miscellaneous areas within the city of Oak Ridge.

  3. Wildlife and Wildlife Habitat Loss Assessment at Green Peter-Foster Project; Middle Fork Santiam River, Oregon, 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1986-02-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Green Peter-Foster Dam and Reservoir Project on the Middle Fork Santiam River, Oregon, to determine losses or gains resulting from the development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types at the project site were mapped based on aerial photographs from 1955, 1972, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Eleven wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Green Peter-Foster Project extensively altered or affected 7873 acres of land and river in the Santiam River drainage. Impacts to wildlife centered around the loss of 1429 acres of grass-forb vegetation, 768 acres of shrubland, and 717 acres of open conifer forest cover types. Impacts resulting from the Green Peter-Foster Project included the loss of critical winter range for black-tailed deer and Roosevelt elk, and the loss of year-round habitat for deer, upland game birds, river otter, beaver, pileated woodpecker, and many other wildlife species. Bald eagle and osprey were benefited by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Green Peter-Foster Project. Losses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  4. Strong Arguments: Rejoinder to Thompson.

    ERIC Educational Resources Information Center

    Knapp, Thomas R.; Sawilowsky, Shlomo S.

    2001-01-01

    Replies to Bruce Thompson's positions on research methodology and editorial policy, addressing each of these issues in the ongoing discussion: (1) structure coefficients; (2) stepwise regression; (3) test reliability; (4) effect sizes; and (5) meta-analysis. (SLD)

  5. Confederated Tribes of the Umatilla Indian Reservation North Fork John Day River Basin Anadromous Fish Enhancement Project, Annual Report for FY 2000.

    SciTech Connect

    Macy, Tom L.; James, Gary A.

    2003-03-01

    The CTUIR North Fork John Day River Basin Anadromous Enhancement Project (NFJDAFEP) identified and prioritized stream reaches in The North Fork John day River basin for habitat improvements during the 2000 project period. Public out reach was emphasized during this first year of the project. We presented multiple funding and enhancement options to landowners. We concentrated on natural recovery methods, riparian fencing and off-stream livestock water developments. Under this BPA contract four riparian easements were signed protecting almost 5 miles of tributary streams. There are nine offstream water developments associated with these easements. Some landowners chose to participate in other programs based on Tribal outreach efforts. Two landowners chose NRCS programs for enhancement and one chose OWEB as a funding source. Two landowners implemented there own enhancement measures protecting 3 miles of stream. Cooperation between the NRCS/FSA/SWCDs and the Tribe to create joint projects and develop alternative funding scenarios for riparian enhancement was a major effort. The Tribe also worked with the North Fork John Day Watershed Council, USFS and ODFW to coordinate projects and support similar projects throughout the John Day Basin. We provided input to the John Day Summary prepared for the NWPPC by ODFW. The Tribe worked with the Umatilla National Forest on the Clear Creek Dredgetailings Rehabilitation project and coordinated regularly with USFS Fisheries, Hydrology and Range staff.

  6. Confederated Tribes of the Umatilla Indian Reservation North Fork John Day River Basin Anadromous Fish Enhancement Project, Annual Report for FY 2001.

    SciTech Connect

    Macy, Tom L.; James, Gary A.

    2003-03-01

    The CTUIR North Fork John Day River Basin Anadromous Enhancement Project (NFJDAFEP) identified and prioritized stream reaches in The North Fork John day River basin for habitat improvements during the 2000 project period. Public outreach was emphasized during this first year of the project. During the past year we concentrated on satisfying landowner needs, providing cost share alternatives, providing joint projects and starting implementation. We presented multiple funding and enhancement options to landowners. We concentrated on natural recovery methods, riparian fencing and offstream livestock water developments. Under this BPA contract four riparian easements have been signed protecting almost 5 miles of tributary streams. There are nine offstream water developments associated with these easements. Some landowners chose to participate in other programs based on Tribal outreach efforts. Some landowners chose NRCS programs for enhancement and others chose OWEB as a funding source. The exact amount of stream protection due to other funding sources probably exceeds that by BPA, however most would not have entered any program without initial Tribal outreach. Cooperation between the NRCS/FSA/SWCDs and the Tribe to create joint projects and develop alternative funding scenarios for riparian enhancement was a major effort. The Tribe also worked with the North Fork John Day Watershed Council, USFS and ODFW to coordinate projects and support similar projects throughout the John Day Basin.

  7. Solar project description for Design Construction Association single family dwelling, Big Fork, Montana

    NASA Astrophysics Data System (ADS)

    1980-04-01

    A solar energy system was installed in a 2100 sq ft house located in Big Fork, Montana. The system is designed to provide solar energy for heating and domestic hot water. Solar energy is collected by flat plate collectors with a gross area of 792 square feet. The collector banks are mounted on the roof of the house and face due south at an angle of 45 deg to the horizontal optimizing solar energy collection. Solar energy is transferred from the collector array to a 1500 gallon storage tank. Water is used as the heat collection, transfer and storage medium. Freeze protection is provided by use of a drain down system. Space heating demands are met by circulating hot water from storage through baseboard units in the distribution system of the house. Auxiliary space heating is provided by an electrical heating element in the boiler. Similarly, an electrical heating element in the DHW tank provides energy for water heating. The dwelling was fully instrumented for performance evaluation since October 1977 and the data is integrated into the National Solar Data Network.

  8. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A.; Vanlew, K.; Melsheimer, T.; Melsheimer, L.; Rideout, C.; Patterson, T.

    1997-12-01

    A second observatory of the Telescopes in Education (TIE) project is in the planning stages, with hopes to be in use by fall 1998. The Little Thompson Observatory will be located adjacent to Berthoud High School in northern Colorado. TIE has offered the observatory a Tinsley 18" Cassegrain telescope on a 10-year loan. Local schools and youth organizations will have prioritized access to the telescope until midnight; after that, the telescope will be open to world-wide use by schools via the Internet. The first TIE observatory is a 24" telescope on Mt. Wilson, already booked through July 1998. That telescope has been in use every clear night for the past four years by up to 50 schools per month. Students remotely control the telescope over the Internet, and then receive the images on their local computers. The estimated cost of the Little Thompson Observatory is roughly \\170,000. However, donations of labor and materials have reduced the final price tag closer to \\40,000. Habitat for Humanity is organized to construct the dome, classrooms, and other facilities. Tom and Linda Melsheimer, who developed the remote telescope control system for the University of Denver's Mount Evans Observatory, are donating a similar control system. The formally-trained, all-volunteer staff will be comprised of local residents, teachers and amateur astronomers. Utilities and Internet access will be provided by the Thompson School District.

  9. Summary of Environmental Flow Monitoring for the Sustainable Rivers Project on the Middle Fork Willamette and McKenzie Rivers, Western Oregon, 2014–15

    USGS Publications Warehouse

    Jones, Krista L.; Mangano, Joseph F.; Wallick, J. Rose; Bervid, Heather D.; Olson, Melissa; Keith, Mackenzie K.; Bach, Leslie

    2016-11-07

    This report presents the results of an ongoing environmental flow monitoring study by The Nature Conservancy (TNC), U.S. Army Corps of Engineers (USACE), and U.S. Geological Survey in support of the Sustainable Rivers Project (SRP) of TNC and USACE. The overarching goal of this study is to evaluate and characterize relations between streamflow, geomorphic processes, and black cottonwood (Populus trichocarpa) recruitment on the Middle Fork Willamette and McKenzie Rivers, western Oregon, that were hypothesized in earlier investigations. The SRP can use this information to plan future monitoring and scientific investigations, and to help mitigate the effects of dam operations on streamflow regimes, geomorphic processes, and biological communities, such as black cottonwood forests, in consultation with regional experts. The four tasks of this study were to:Compare the hydrograph from Water Year (WY) 2015 with hydrographs from WYs 2000–14 and the SRP flow recommendations,Assess short-term and system-wide changes in channel features and vegetation throughout the alluvial valley section of the Middle Fork Willamette River (2005–12),Examine changes in channel features and vegetation over two decades (1994–2014) for two short mapping zones on the Middle Fork Willamette and McKenzie Rivers, andComplete a field investigation of summer stage and the growth of black cottonwood and other vegetation on the Middle Fork Willamette and McKenzie Rivers in summer 2015.

  10. Along Middle Fork Road toward North Fork of the Crazy ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Along Middle Fork Road toward North Fork of the Crazy Woman Creek Bridge, view to west - North Fork of Crazy Woman Creek Bridge, Spanning North Fork of Crazy Woman Creek at Middle Fork Road, Buffalo, Johnson County, WY

  11. Design criteria applied for the Lower Pressure Tunnel of the North Fork Stanislaus River Hydroelectric Project in California

    NASA Astrophysics Data System (ADS)

    Schleiss, A.

    1988-07-01

    The application of various criteria and certain new approaches to design is illustrated by the example of the Lower Collierville Pressure Tunnel of the North Fork Stanislaus River Hydropower Project in California. With a maximum internal water pressure of 72 bar, Lower Collierville Tunnel will be, when commissioned in 1989, the highest stressed pressure tunnel in the world not situated in granitic rocks. The geological conditions and the results of the geotechnical investigations are described briefly. For the steel-lined portion of the tunnel, the approach for determining the bearing capacity of the rock mass and the load sharing between steel and rock is discussed. The required length of steel liner was determined on the basis of rock mechanical (hydraulic jacking) and rock hydraulic (seepage losses and extension of saturated zone due to seepage) criteria. The use of a new theory allows the effects of mechanical-hydraulic interaction to be taken into account. Finally the methods of estimating the expected water losses and the sealing effect of the consolidation grouting are described.

  12. EPA Region 10 Climate Change and TMDL Pilot Project - South Fork Nooksack River, Washington

    EPA Science Inventory

    The U.S. Environmental Protection Agency (EPA) Region 10 and EPA’s Office of Research and Development (ORD) and Office of Water (OW) have launched a pilot research project to consider how projected climate change impacts could be incorporated into a TMDL and influence restoration...

  13. 76 FR 46721 - Salmon-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-03

    ... Restoration Project Environmental Impact Statement AGENCY: Forest Service, USDA. ACTION: Notice of intent to... National Forest, is proposing an integrated hazardous fuels and forest restoration project in the Upper... authorities and expedited environmental analysis under the Healthy Forest Restoration Act (HFRA) of 2003....

  14. 15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. INSIDE VIEW OF FLUME, LOOKING DOWNSTREAM, LEFT FORK TO SETTLING BASIN, SHOWING RIGHT FORK WITH GATE IN PLACE AND A FEW NEEDLES IN PLACE - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

  15. 1976 Big Thompson flood, Colorado

    USGS Publications Warehouse

    Jarrett, R. D.; Vandas, S.J.

    2006-01-01

    In the early evening of July 31, 1976, a large stationary thunderstorm released as much as 7.5 inches of rainfall in about an hour (about 12 inches in a few hours) in the upper reaches of the Big Thompson River drainage. This large amount of rainfall in such a short period of time produced a flash flood that caught residents and tourists by surprise. The immense volume of water that churned down the narrow Big Thompson Canyon scoured the river channel and destroyed everything in its path, including 418 homes, 52 businesses, numerous bridges, paved and unpaved roads, power and telephone lines, and many other structures. The tragedy claimed the lives of 144 people. Scores of other people narrowly escaped with their lives. The Big Thompson flood ranks among the deadliest of Colorado's recorded floods. It is one of several destructive floods in the United States that has shown the necessity of conducting research to determine the causes and effects of floods. The U.S. Geological Survey (USGS) conducts research and operates a Nationwide streamgage network to help understand and predict the magnitude and likelihood of large streamflow events such as the Big Thompson Flood. Such research and streamgage information are part of an ongoing USGS effort to reduce flood hazards and to increase public awareness.

  16. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2003-05-01

    The Little Thompson Observatory is the first community-built observatory that is part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. A committee of teachers and administrators from the Thompson School District have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." In addition, this past summer our program became an accredited course by Colorado State University. Our next project is to partner with the Discovery Center Science Museum and Colorado State University to provide additional teacher education programs. Our training materials have also been shared with TIE/Mt. Wilson, NASA Goddard and Howard University, which are working together to develop a similar teacher education program.

  17. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2002-12-01

    The Little Thompson Observatory is the first community-built observatory that is part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. A committee of teachers and administrators from the Thompson School District have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." In addition, this past summer our program became an accredited course by Colorado State University. Our next project is to partner with the Discovery Center Science Museum and Colorado State University to provide additional teacher education programs. Our training materials have also been shared with TIE/Mt. Wilson, NASA Goddard and Howard University, which are working together to develop a similar teacher education program.

  18. Technology to the People: Kirsten Thompson--Milwaukee Public Library

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    Considering Kirsten Thompson's career in the Milwaukee Public Library (MPL), it is no surprise that as an undergraduate she studied both computer programming and psychology. The combination is the driving force behind her multiple projects at MPL, which have all focused on bringing technology to users. "Having the computers is great, but you need…

  19. Value engineering study report on Lower East Fork Poplar Creek Project. Alternative No. 3

    SciTech Connect

    1995-08-01

    The project under study is Alternative No. 3 as identified in the Feasibility Study dated August 1994. This alternative is identified as Excavation and Disposal of Commercial/DOE, Other, and Residential Remedial Unit Soil. The assumptions used for generating baseline costs are discussed in site associated costs. It is further described as follows: Soils with mercury concentrations greater than 200 ppM in the Commercial/DOE and Other Remedial Units and greater than 180 ppM in the Residential Remedial Unit [41,300m{sup 3} (54,000yd{sup 3} a volume equivalent to approximately 6,750 dump truck loads)] would be excavated and disposed of in an approved, lined landfill at Y-12 with leachate collection and possible pretreatment of the leachate before discharge. Because 0.6 ha (1.5 acres) of wetland would be destroyed, wetlands banking would occur, whereby a 1.8-ha (4.5-acre) wetland would be constructed on DOE-owned land near K-25. Borrow soil would be obtained from the Y-12 West End Borrow Area or from excess soil located at Y-12 landfills to fill the excavation. It is estimated that 7.3 ha (18.2 acres, and area about the size of 17 football fields) of habitat would be adversely affected. This alternative would use BMPs to minimize any adverse affects and to comply substantively with regulatory requirements.

  20. Thompson receives 1994 Bowen award

    NASA Astrophysics Data System (ADS)

    Green, David H.; Thompson, Alan Bruce

    At the Spring Meeting in Baltimore, May 23, 1994, Alan Bruce Thompson of the Eidgenossiche Technische Hochschule, Zurich, received the 1994 N. L. Bowen Award of the Volcanology, Geochemsitry, and Petrology section, which is given for a single outstanding contribution to volcanology, geochemistry, or petrology made during the preceding 5 years. The award was presented by David H. Green of the Research School of Earth Sciences. The citation and response are given here.

  1. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2004-05-01

    The Little Thompson Observatory is the first community-built E/PO observatory that is accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. The observatory is located on the grounds of Berthoud High School in northern Colorado. The observatory will celebrate its fifth anniversary in summer 2004, and we are planning to expand the building to accommodate our growing number of visitors! We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. We have recently submitted ROSS E/PO proposals toward future teacher programs. A committee of teachers and administrators from the Thompson School District selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Our program is also accredited by Colorado State University.

  2. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2003-12-01

    The Little Thompson Observatory is the first community-built observatory that is part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. A committee of teachers and administrators from the Thompson School District selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Our program is also accredited by Colorado State University.

  3. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.

    2002-05-01

    The Little Thompson Observatory is believed to be the first of its kind, located next to a high school and accessible to other schools remotely over the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction was done completely by volunteer labor, and the observatory was built on the grounds of Berthoud High School in northern Colorado. During 2001, we averaged 400-500 visitors per month. We are grateful to have received a STScI IDEAS grant to provide teacher training workshops for K-12 schools in northern Colorado to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. We are honored that a committee of teachers and administrators from the Thompson School district have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Also in the past year, our training materials have been shared with NASA Goddard and Howard University, which are working together to develop a similar teacher education program. Our next goal is to add solar observing capability! Please visit our website at www.starkids.org.

  4. Telescopes in Education: the Little Thompson Observatory

    NASA Astrophysics Data System (ADS)

    Schweitzer, A. E.; Melsheimer, T. T.; Sackett, C.

    2001-12-01

    The Little Thompson Observatory is believed to be the first observatory built as part of a high school and accessible to other schools remotely, via the Internet. This observatory is the second member of the Telescopes in Education (TIE) project. Construction of the building was done completely by volunteer labor, and first light occurred in May 1999. The observatory is located on the grounds of Berthoud High School in northern Colorado. We are grateful to have received an IDEAS grant to provide teacher training workshops for K-12 schools in Colorado to make use of the observatory, including remote observing from classrooms. Students connect to the observatory over the Internet, and then receive the images back on their local computers. We are honored that a committee of teachers and administrators from the Thompson School district have selected these workshops to count towards Incentive Credits (movement on the salary schedule) because the course meets the criteria: "Learning must be directly transferable to the classroom with students and relate to standards, assessment and/or technology." Also in the past year, our training materials have been shared with NASA Goddard and Howard University, which are working together to develop a similar teacher education program.

  5. Wildlife and Wildlife Habitat Loss Assessment at Hills Creek Dam and Reservoir Project, Middle Fork Willamette River, Oregon, 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Hills Creek Dam and Reservoir Project on the Middle Fork Willamette River, Oregon, to determine losses or gains resulting from the development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1944, 1964, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Fifteen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Hills Creek Project extensively altered or affected 4662 acres of land and river in the Middle Fork Willamette River drainage. Impacts to wildlife centered around the loss of 2694 acres of old-growth forest and 207 acres of riparian habitat. Impacts resulting from the Hills Creek Project included the loss of winter range for Roosevelt elk, and the loss of year-round habitat for black-tailed deer, black bear, cougar, river otter, beaver, ruffed grouse, spotted owl, and other nongame species. Bald eagle and osprey were benefited by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Hills Creek Project, losses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  6. Wildlife and Wildlife Habitat Loss Assessment Summary at Lookout Point Dam and Reservoir Project, Middle Fork Willamette River, Oregon; 1985 Final Report.

    SciTech Connect

    Bedrossian, K.L.; Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Lookout Point Dam and Reservoir Project on the Middle Fork Willamette River, Oregon, to determine losses or gains resulting from development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1944, 1956, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Seventeen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Lookout Point Project extensively altered or affected 6790 acres of land and river in the Middle Fork Willamette River drainage. Impacts to wildlife centered around the loss of 724 acres of old-growth conifer forest and 118 acres of riparian habitat. Impacts resulting from the Lookout Point Project included the loss of winter range for Roosevelt elk, and the loss of year-round habitat for black-tailed deer, western gray squirrel, red fox, mink, beaver, ruffed grouse, ring-necked pheasant, California quail, spotted owl, and other nongame species. Bald eagle and osprey were benefitted by an increase in foraging habitat. The potential of the affected area to support wildlife was greatly altered as a result of the Lookout Point Project. Loses or gains in the potential of the habitat to support wildlife will exist over the life of the project.

  7. Intraoperative value of the thompson test.

    PubMed

    Cuttica, Daniel J; Hyer, Christopher F; Berlet, Gregory C

    2015-01-01

    The purpose of the present study was to assess the validity of the Thompson sign and determine whether the deep flexors of the foot can produce a falsely intact Achilles tendon.Ten unmatched above-the-knee lower extremity cadaveric specimens were studied. In group 1, the Achilles tendon was sectioned into 25% increments. The Thompson maneuver was performed after each sequential sectioning of the Achilles tendon, including after it had been completely sectioned. If the Thompson sign was still intact after complete release of the Achilles tendon, we proceeded to release the tendon, and tendon flexor hallucis longus, flexor digitorum longus, and posterior tibial tendons. The Thompson test was performed after the release of each tendon. In group 2, the tendon releases were performed in a reverse order to that of group 1, with the Thompson test performed after each release. In group 1, the Thompson sign remained intact in all specimens after sectioning of 25%, 50%, and 75% of the tendon. After complete (100%) release of the tendon, the Thompson sign was absent in all specimens. In group 2, the Thompson sign remained intact after sectioning of the posterior tibial, flexor digitorum longus, and flexor hallucis longus tendons in all specimens. The Thompson sign remained intact in all specimens after sectioning of 25%, 50%, and 75% of the Achilles tendon. After complete release of the tendon, the Thompson sign was absent in all specimens.The Thompson test is an accurate clinical test for diagnosing complete Achilles tendon ruptures. However, it might not be a useful test for diagnosing partial Achilles tendon ruptures. Our findings also call into question the usefulness of the Thompson test in the intraoperative setting.

  8. 78 FR 29202 - Environmental Impact Statement: Grand Forks County, North Dakota and Polk County, Minnesota

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-17

    ... Federal Highway Administration Environmental Impact Statement: Grand Forks County, North Dakota and Polk... prepared for a proposed highway project in Grand Forks County, North Dakota and Polk County, Minnesota. FOR... Sorlie Bridge over the Red River between Grand Forks, North Dakota and East Grand Forks,...

  9. Donald O. Thompson: A remembrance

    NASA Astrophysics Data System (ADS)

    Achenbach, J. D.; Alers, G.; Schmerr, Lester W., Jr.; Bond, Leonard J.

    2014-02-01

    This paper was developed from the remarks delivered to honor Don Thompson by the banquet speakers at the 40th QNDE meeting, July 2013. Don died peacefully at his home just days later on July 29th after a two year battle with cancer. "Don was a tenacious fighter for what he believed in, and his vision and perseverance did much to establish NDE in both the US and wider global R&D community. He will be greatly missed by his many friends and colleagues in the NDE community".

  10. An archaeological reconnaissance of a 14 mile section of the East Fork Poplar Creek for the Environmental Restoration Project, Anderson and Roane Counties, Tennessee

    SciTech Connect

    DuVall, G.D.

    1993-01-01

    At the request of the US Army Corps of Engineers, Nashville District, Nashville, Tennessee, an archaeological reconnaissance of the potential impact areas of the Environmental Restoration Project (ERP) along the East Fork Poplar Creek was conducted during the period December 16, 1991, and March 3, 1992. The reconnaissance was conducted in response to environmental evaluations as a result of the accidental spillage of approximately 293,000 pounds of mercury, radionuclides, heavy metals and other compounds. The reconnaissance to assess adverse impacts to cultural resources located within the boundaries of Federally-licensed, permitted, funded or assisted projects was conducted in compliance with the National Historic Preservation Act of 1966, National Environmental Policy Act of 1969 and Executive Order 11593.

  11. Tuning fork decay.

    PubMed

    Miller, G W

    1979-03-01

    Tuning fork tests are used routinely by many otologists. A different group of otologists find the tests inconsistent and unreliable. This controversy has probably developed because the audiometer has replaced the tuning fork in hearing measurement. As a result, the art of use of the tuning fork is poorly learned. This study examines decay, one of the physical parameters of tuning forks. Measurements of acoustic (sound wave) and vibration (stem movement) decay were made. Alteration in decay due to pressure changes on the fork stem were studied. Acoustic signals were generated in an anechoic chamber. Vibration measurements were obtained using an artificial mastoid. Analysis of the signals was accomplished by a system of amplifiers, filters, tape recorders, and a graphic recorder. Tuning fork sound decay is a property of the instrument which occurs every time the fork is struck. The decay is a constant in decibels per second. The acoustic mode and the vibration mode decay at similar rates for the same fork. The strike frequency (a higher frequency than the fundamental produced when the fork is struck) also has a constant decay rate in decibels per second, and it is reported here for the first time. Force of 800 gm. and less applied to the bottom of the stem in vibration measurement caused minimal decay constant changes. When the physical parameters of the tuning fork (including this information on damping) are fully studied, tuning fork testing should become more of a science and less of an art.

  12. Environmental compliance plan for the Lower East Fork Poplar Creek Remedial Action Project at Oak Ridge, Tennessee

    SciTech Connect

    1996-07-01

    Remedial action for Lower East Fork Poplar Creek, as defined by the Record of Decision, requires that soil contaminated with >400 ppM mercury be excavated and disposed. Based on the remediation goal, soil will be excavated from areas located at the NOAA site and the Bruner site and disposed at the Industrial Landfill V at the Y-12 Plant. Objective is to minimize the risk to human health and the environment from contaminated soil in the lower EFPC floodplain pursuant to CERCLA and the Federal Facility Agreement (DOE 1992).

  13. The Little Thompson Observatory's Astronomy Education Programs

    NASA Astrophysics Data System (ADS)

    Schweitzer, Andrea E.

    2007-12-01

    The Little Thompson Observatory is a community-built E/PO observatory and is a member of the Telescopes in Education (TIE) project. The observatory is located on the grounds of Berthoud High School in northern Colorado. Annually we have approximately 5,000 visitors, which is roughly equal to the population of the small town of Berthoud, CO. This past year, we have used the funding from our NASA ROSS E/PO grant to expand our teacher workshop programs, and included the baseball-sized meteorite that landed in Berthoud three years ago. Our teacher programs have involved scientists from the Southwest Research Institute and from Fiske Planetarium at CU-Boulder. We thank the NASA ROSS E/PO program for providing this funding! We also held a Colorado Project ASTRO-GEO workshop, and the observatory continues to make high-school astronomy courses available to students from the surrounding school districts. Statewide, this year we helped support the development and construction of three new educational observatories in Colorado, located in Estes Park, Keystone, and Gunnison. The LTO is grateful to have received the recently-retired 24-inch telescope from Mount Wilson Observatory as part of the TIE program. To provide a new home for this historic telescope, we have doubled the size of the observatory and are building a second dome (all with volunteer labor). During 2008 we plan to build a custom pier and refurbish the telescope.

  14. Wildlife and Wildlife Habitat Loss Assessment at Cougar Dam and Reservoir Project, South Fork McKenzie River, Oregon; 1985 Final Report.

    SciTech Connect

    Noyes, J.H.

    1985-09-01

    A habitat based assessment was conducted of the US Army Corps of Engineers' Cougar Dam and Reservoir Project on the South Fork McKenzie River, Oregon, to determine losses or gains resulting from the development and operation of the hydroelectric related components of the project. Preconstruction, postconstruction, and recent vegetation cover types of the project site were mapped based on aerial photographs from 1953, 1965, and 1979, respectively. Vegetation cover types were identified within the affected area and acreages of each type at each period were determined. Fifteen wildlife target species were selected to represent a cross-section of species groups affected by the project. An interagency team evaluated the suitability of the habitat to support the target species at each time period. An evaluation procedure which accounted for both the quantity and quality of habitat was used to aid in assessing impacts resulting from the project. The Cougar Project extensively altered or affected 3096 acres of land and river in the McKenzie River drainage. Impacts to wildlife centered around the loss of 1587 acres of old-growth conifer forest and 195 acres of riparian hardwoods. Impacts resulting from the Cougar Project included the loss of winter range for Roosevelt elk, and the loss of year-round habitat for black-tailed deer, black bear, cougar, river otter, beaver, spotted owl, and other nongame species. Bald eagle and osprey were benefited by an increase in foraging habitat. The potential of the effected area to support wildlife was greatly altered as a result of the Cougar Project. Loses or grains in the potential of the habitat to support wildlife will exist over the life of the project.

  15. 4. VIEW UPSTREAM ALONG NORTH FORK OF MIDDLE FORK OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW UPSTREAM ALONG NORTH FORK OF MIDDLE FORK OF TULE RIVER, LOOKING TOWARD DOWNSTREAM SIDE OF BRIDGE AND POWERHOUSE; FLUME AT LEFT. LOOKING EAST. 90mm lens - Tule River Hydroelectric Complex, Tule River Bridge, Spanning North Fork of Middle Fork of Tule River, Springville, Tulare County, CA

  16. The Little Thompson Observatory's Astronomy Education Programs

    NASA Astrophysics Data System (ADS)

    Schweitzer, Andrea E.

    2008-05-01

    The Little Thompson Observatory is a community-built E/PO observatory and is a member of the Telescopes in Education (TIE) project. The observatory is located on the grounds of Berthoud High School in northern Colorado. Annually we have approximately 5,000 visitors, which is roughly equal to the population of the small town of Berthoud, CO. In spring 2008, we offered a special training session to boost participation in the GLOBE at Night international observing program. During 2005-2007 we used the funding from our NASA ROSS E/PO grant to expand our teacher workshop programs, and included the baseball-sized meteorite that landed in Berthoud four years ago. Our teacher programs are ongoing, and include scientists from the Southwest Research Institute and from Fiske Planetarium at CU-Boulder. We thank the NASA ROSS E/PO program for providing this funding! Statewide, we are a founding member of Colorado Project ASTRO-GEO, and the observatory offers high-school astronomy courses to students from the surrounding school districts. We continue to support the development and construction of three new educational observatories in Colorado, located in Estes Park, Keystone and Gunnison. The LTO is grateful to have received the retired 24-inch telescope from Mount Wilson Observatory as part of the TIE program. To provide a new home for this historic telescope, we have doubled the size of the observatory and are building a second dome (almost all construction done with volunteer labor). During 2008 we will be building a custom pier and refurbishing the telescope.

  17. 9. 'CRIB DAM IN LAKE FORK RIVER AT HEADING OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. 'CRIB DAM IN LAKE FORK RIVER AT HEADING OF LAKE FORK CANAL, UINTAH PROJECT. TWO SLUICEWAYS TWENTY FEET WIDE HAVE BEEN LEFT IN THE DAM TO PASS BOULDERS DURING HIGH WATER. THESE SLUICEWAYS ARE CLOSED BY LOGS AND HAY DURING LOW WATER.' Date unknown - Irrigation Canals in the Uinta Basin, Duchesne, Duchesne County, UT

  18. 75 FR 54601 - Lower South Fork LLC; Notice of Application Accepted for Filing and Soliciting Comments, Motions...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-08

    ... Energy Regulatory Commission Lower South Fork LLC; Notice of Application Accepted for Filing and...-000. c. Date filed: April 19, 2010 and supplemented July 28, 2010. d. Applicant: Lower South Fork LLC. e. Name of Project: Lower South Fork Irrigation Project. f. Location: The project is located...

  19. A Reply? A Response to Penny Thompson

    ERIC Educational Resources Information Center

    Doble, Peter

    2007-01-01

    Penny Thompson's "reply" to the author's article (Doble, 2005) briefly tells readers that for an answer to some of the author's queries, readers may turn to her book; for the rest, she proposes to take "the argument" further. One of the problems with her earlier article was that it had no discernible argument, so it is not easy to see how it may…

  20. Forks in the Road: The Many Paths of Arts Alumni--Strategic National Arts Alumni Project 2010 Findings

    ERIC Educational Resources Information Center

    Strategic National Arts Alumni Project, 2011

    2011-01-01

    More than 120,000 visual and performing arts degrees are granted each year (Americans for the Arts, 2010) and stereotypes abound about what happens to these people. One common view is that few make a living doing art and are dissatisfied with their education and career opportunities. Findings from the Strategic National Arts Alumni Project (SNAAP)…

  1. Paresev in flight with pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1964-01-01

    This movie clip runs 37 seconds in length and begins with a shot from the chase plane of NASA Dryden test pilot Milt Thompson at the controls of the Paresev, then the onboard view from the pilot's seat and finally bringing the Paresev in for a landing on the dry lakebed at Edwards AFB. The Paresev (Paraglider Rescue Vehicle) was an indirect outgrowth of kite-parachute studies by NACA Langley engineer Francis M. Rogallo. In early 1960's the 'Rogallo wing' seemed an excellent means of returning a spacecraft to Earth. The delta wing design was patented by Mr. Rogallo. In May 1961, Robert R. Gilruth, director of the NASA Space Task Group, requested studies of an inflatable Rogallo-type 'Parawing' for spacecraft. Several companies responded; North American Aviation, Downey, California, produced the most acceptable concept and development was contracted to that company. In November 1961 NASA Headquarters launched a paraglider development program, with Langley doing wind tunnel studies and the NASA Flight Research Center supporting the North American test program. The North American concept was a capsule-type vehicle with a stowed 'parawing' that could be deployed and controlled from within for a landing more like an airplane instead of a 'splash down' in the ocean. The logistics became enormous and the price exorbitant, plus NASA pilots and engineers felt some baseline experience like building a vehicle and flying a Parawing should be accomplished first. The Paresev (Paraglider Research Vehicle) was used to gain in-flight experience with four different membranes (wings), and was not used to develop the more complicated inflatable deployment system. The Paresev was designed by Charles Richard, of the Flight Research Center Vehicle and System Dynamics Branch, with the rest of the team being: engineers, Richard Klein, Gary Layton, John Orahood, and Joe Wilson; from the Maintenance and Manufacturing Branch: Frank Fedor, LeRoy Barto; Victor Horton as Project Manager, with

  2. Paresev in flight with pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1964-01-01

    This movie clip runs 37 seconds in length and begins with a shot from the chase plane of NASA Dryden test pilot Milt Thompson at the controls of the Paresev, then the onboard view from the pilot's seat and finally bringing the Paresev in for a landing on the dry lakebed at Edwards AFB. The Paresev (Paraglider Rescue Vehicle) was an indirect outgrowth of kite-parachute studies by NACA Langley engineer Francis M. Rogallo. In early 1960's the 'Rogallo wing' seemed an excellent means of returning a spacecraft to Earth. The delta wing design was patented by Mr. Rogallo. In May 1961, Robert R. Gilruth, director of the NASA Space Task Group, requested studies of an inflatable Rogallo-type 'Parawing' for spacecraft. Several companies responded; North American Aviation, Downey, California, produced the most acceptable concept and development was contracted to that company. In November 1961 NASA Headquarters launched a paraglider development program, with Langley doing wind tunnel studies and the NASA Flight Research Center supporting the North American test program. The North American concept was a capsule-type vehicle with a stowed 'parawing' that could be deployed and controlled from within for a landing more like an airplane instead of a 'splash down' in the ocean. The logistics became enormous and the price exorbitant, plus NASA pilots and engineers felt some baseline experience like building a vehicle and flying a Parawing should be accomplished first. The Paresev (Paraglider Research Vehicle) was used to gain in-flight experience with four different membranes (wings), and was not used to develop the more complicated inflatable deployment system. The Paresev was designed by Charles Richard, of the Flight Research Center Vehicle and System Dynamics Branch, with the rest of the team being: engineers, Richard Klein, Gary Layton, John Orahood, and Joe Wilson; from the Maintenance and Manufacturing Branch: Frank Fedor, LeRoy Barto; Victor Horton as Project Manager, with

  3. 4. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shoreof Clark Fork River showing 4 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  4. 2. View of Clark Fork Vehicle Bridge facing northeast. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View of Clark Fork Vehicle Bridge facing northeast. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  5. 1. View of Clark Fork Vehicle Bridge facing west. Panorama ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View of Clark Fork Vehicle Bridge facing west. Panorama showing the entire span of bridge from north shore of the Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  6. 7. View of Clark Fork Vehicle Bridge facing northwest. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. View of Clark Fork Vehicle Bridge facing northwest. Bridge from south shore of Clark Fork River showing 4 1/2 spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  7. 3. View of Clark Fork Vehicle Bridge facing southwest. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. View of Clark Fork Vehicle Bridge facing southwest. Bridge from north shore of Clark Fork River. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  8. Nutrient Loading and Algal Response in West Thompson Lake, Thompson, Connecticut, 2003-2005

    USGS Publications Warehouse

    Morrison, Jonathan; Colombo, Michael J.

    2008-01-01

    Water quality and nutrient loads were characterized for parts of the Quinebaug River and West Thompson Lake in northeastern Connecticut during 2003 to 2005. The West Thompson Lake watershed is a mainly forested watershed that receives treated municipal wastewater from several point sources in Massachusetts. The lake is a flood-control reservoir formed in 1966 by impoundment of the Quinebaug River. Median concentrations of total phosphorus in two inflow (upstream) and one outflow (downstream) sampling stations on the Quinebaug River were higher than the nutrient criteria recommended by the U.S. Environmental Protection Agency (USEPA) for rivers and streams in aggregate Ecoregion XIV. In general, concentrations of total phosphorus in West Thompson Lake also were above the nutrient criteria recommended by USEPA for lakes and impoundments in aggregate Ecoregion XIV. The trophic status of West Thompson Lake has changed since 1995 from a hypereutrophic lake to a eutrophic lake; however, the lake still has large algal blooms. These blooms are predominated by blue-green algae, with chlorophyll-a concentrations of more than 30 micrograms per liter and algal cell counts as high as 73,000 cells/mL. Water samples collected during the summer of 2005 identified phosphorus as the primary limiting nutrient early in the season, but algal growth is probably co-limited by phosphorus and nitrogen later in the season. Lake-bottom sediments were collected from several areas throughout the lake and ranged in thickness from less than 1 foot (ft) to more than 3 ft. Concentrations of phosphorus in sediments differed throughout the lake; the highest values were found in the middle of the lake. Concentrations of total phosphorus also increased from an average 1,800 milligrams per kilogram (mg/kg) in the upper layers of sediment to more than 6,000 mg/kg at depth in the sediment. Annual, seasonal, and monthly loads and yields of nutrients were calculated for the three sampling locations on the

  9. Outbreak of Salmonella Thompson infection in a Swedish dairy herd.

    PubMed

    Sternberg, S; Johnsson, A; Aspan, A; Bergström, K; Kallay, T B; Szanto, E

    2008-11-15

    Salmonella Typhimurium was isolated from a faecal sample from a cow in a Swedish dairy herd after calving. When investigations were undertaken in the herd, Salmonella Thompson was isolated from heifers on a separate pasture, and when these heifers were brought into the herd S Thompson spread rapidly. Control strategies managed to rid the herd of the S Typhimurium infection and the prevalence of S Thompson was at first substantially reduced. There was a rapid increase in its prevalence when the animals were let out to pasture and this development eventually led to the depopulation of the entire herd. PMID:19011246

  10. X-15 mock-up with test pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1993-01-01

    NASA research pilot Milt Thompson is seen here with the mock-up of X-15 #3 that was later installed at the NASA Dryden Flight Research Center, Edwards, California. Milton 0. Thompson was a research pilot, Chief Engineer and Director of Research Projects during a long career at the NASA Dryden Flight Research Center. Thompson was hired as an engineer at the flight research facility on 19 March 1956, when it was still under the auspices of NACA. He became a research pilot on 25 May 1958. Thompson was one of the 12 NASA, Air Force, and Navy pilots to fly the X-15 rocket-powered research aircraft between 1959 and 1968. He began flying X-15s on 29 October 1963. He flew the aircraft 14 times during the following two years, reaching a maximum speed of 3723 mph (Mach 5.42) and a peak altitude of 214,100 feet on separate flights. (On a different flight, he reached a Mach number of 5.48 but his mph was only 3712.) Thompson concluded his active flying career in 1968, becoming Director of Research Projects. In 1975 he was appointed Chief Engineer and retained the position until his death on 8 August 1993. The X-15 was a rocket powered aircraft 50 ft long with a wingspan of 22 ft. It was a missile-shaped vehicle with an unusual wedge-shaped vertical tail, thin stubby wings, and unique side fairings that extended along the side of the fuselage. The X-15 weighed about 14,000 lb empty and approximately 34,000 lb at launch. The XLR-99 rocket engine, manufactured by Thiokol Chemical Corp., was pilot controlled and was capable of developing 57,000 lb of thrust. North American Aviation built three X-15 aircraft for the program. The X-15 research aircraft was developed to provide in-flight information and data on aerodynamics, structures, flight controls, and the physiological aspects of high-speed, high-altitude flight. A follow on program used the aircraft as a testbed to carry various scientific experiments beyond the Earth's atmosphere on a repeated basis. For flight in the dense

  11. X-15 mock-up with test pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1993-01-01

    NASA research pilot Milt Thompson stands next to a mock-up of X-15 number 3 that was later installed at the NASA Dryden Flight Research Center, Edwards, California. Milton 0. Thompson was a research pilot, Chief Engineer and Director of Research Projects during a long career at the NASA Dryden Flight Research Center. Thompson was hired as an engineer at the flight research facility on 19 March 1956, when it was still under the auspices of NACA. He became a research pilot on 25 May 1958. Thompson was one of the 12 NASA, Air Force, and Navy pilots to fly the X-15 rocket-powered research aircraft between 1959 and 1968. He began flying X-15s on 29 October 1963. He flew the aircraft 14 times during the following two years, reaching a maximum speed of 3723 mph (Mach 5.42) and a peak altitude of 214,100 feet on separate flights. Thompson concluded his active flying career in 1968, becoming Director of Research Projects. In 1975 he was appointed Chief Engineer and retained the position until his death on 8 August 1993. The X-15 was a rocket-powered aircraft 50 ft long with a wingspan of 22 ft. It was a missile-shaped vehicle with an unusual wedge-shaped vertical tail, thin stubby wings, and unique side fairings that extended along the side of the fuselage. The X-15 weighed about 14,000 lb empty and approximately 34,000 lb at launch. The XLR-99 rocket engine, manufactured by Thiokol Chemical Corp., was pilot controlled and was capable of developing 57,000 lb of thrust. North American Aviation built three X-15 aircraft for the program. The X-15 research aircraft was developed to provide in-flight information and data on aerodynamics, structures, flight controls, and the physiological aspects of high-speed, high-altitude flight. A follow-on program used the aircraft as a testbed to carry various scientific experiments beyond the Earth's atmosphere on a repeated basis. For flight in the dense air of the usable atmosphere, the X-15 used conventional aerodynamic controls such as

  12. Tuning fork tests: forgotten art.

    PubMed

    Girgis, T F; Shambaugh, G E

    1988-01-01

    Four examples are cited in which tuning fork tests helped in proper selection of patients for surgery, after audiometric air and bone tests were equivocal or gave the wrong diagnostic and prognostic indication.

  13. Tuning Forks and Monitor Screens.

    ERIC Educational Resources Information Center

    Harrison, M. A. T.

    2000-01-01

    Defines the vibrations of a tuning fork against a computer monitor screen as a pattern that can illustrate or explain physical concepts like wave vibrations, wave forms, and phase differences. Presents background information and demonstrates the experiment. (Author/YDS)

  14. 1. Historic American Buildings Survey Thompson & West Illustrator in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey Thompson & West Illustrator in History of Sacramento County Photo Copy 1960 NORTH ELEVATION (1880) - Albert Gallatin House, 1527 H Street, Sacramento, Sacramento County, CA

  15. 1. Historic American Building Survey Thompson & West Illustrated History ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Building Survey Thompson & West Illustrated History of Sacramento Co. Drawing of 1880 Plan & Rephoto 1960 NORTHEAST CORNER AND GENERAL PLAN - Crocker Art Gallery, 216 O Street, Sacramento, Sacramento County, CA

  16. 19. View of Clark Fork Vehicle Bridge facing north. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. View of Clark Fork Vehicle Bridge facing north. Looking at north abutment and underside of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  17. 20. View of Clark Fork Vehicle Bridge facing up. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. View of Clark Fork Vehicle Bridge facing up. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  18. 21. View of Clark Fork Vehicle Bridge facing west. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View of Clark Fork Vehicle Bridge facing west. Looking at bridge deck, guard rail, juncture of two bridge spans. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  19. 22. View of Clark Fork Vehicle Bridge facing downwest side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View of Clark Fork Vehicle Bridge facing down-west side. Looking at road deck and vertical laced channel. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  20. 18. View of Clark Fork Vehicle Bridge facing north. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of Clark Fork Vehicle Bridge facing north. Looking at north concrete abutment and timber stringers. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  1. 12. View of Clark Fork Vehicle Bridge facing south. Approach ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. View of Clark Fork Vehicle Bridge facing south. Approach from the north road. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  2. 11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. View of Clark Fork Vehicle Bridge facing northwest. Southernmost span. Plaque was originally located where striped traffic sign is posted. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  3. 8. View of Clark Fork Vehicle Bridge facing southwest. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. View of Clark Fork Vehicle Bridge facing southwest. Looking at understructure of northernmost span. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  4. Projected ground-water development, ground-water levels, and stream-aquifer leakage in the South Fork Solomon River Valley between Webster Reservoir and Waconda Lake, north-central Kansas, 1979-2020

    USGS Publications Warehouse

    Kume, Jack; Lindgren, R.J.; Stullken, L.E.

    1985-01-01

    A two-dimensional finite difference computer model was used to project changes in the potentiometric surface, saturated thickness, and stream aquifer leakage in an alluvial aquifer resulting from four instances of projected groundwater development. The alluvial aquifer occurs in the South Fork Solomon River valley between Webster Reservoir and Waconda Lake in north-central Kansas. In the first two projections, pumpage for irrigation was held constant at 1978 rates throughout the projection period (1979-2020). In the second two projections, the 1978 pumpage was progressively increased each yr through 2020. In the second and fourth projections, surface water diversions in the Osborne Irrigation Canal were decreased by 50 %. For the third and fourth projections, each grid-block in the modeled area was classified initially as one of six types according to whether it represented irrigable or nonirrigable land, to its saturated thickness, to its location inside or outside the canal-river area, and to its pumping rate. The projected base-flow rates (leakage from the aquifer to the river) were lower during the irrigation season (June, July, and August) than during the other months of the yr because of the decline in hydraulic head produced by groundwater pumpage. Stream depletion, calculated as a decrease below the average (1970-78) estimated winter base-flow rate of 16.5 cu ft/sec, varied inversely with base flow. For the first two projections, a constant annual cycle of well pumpage and recharge was used throughout the projection period. Aquifer leakage to the river was nearly constant by the mid-to-late 1990's, implying that flow conditions had attained a stabilized annual cycle. The third and fourth projections never attained an annual stabilized cycle because the irrigation pumpage rate was increased each year. By the early 1980's, the hydraulic head had fallen below river stage, reversing the hydraulic gradient at the stream-aquifer interface and resulting in net

  5. 77 FR 34337 - Uinta-Wasatch-Cache National Forest; Evanston-Mountain View Ranger District; Utah; Smiths Fork...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-11

    ... Fork Vegetation Restoration Project AGENCY: Forest Service, USDA. ACTION: Correction to notice of... submitting a correction to the notice of intent for the Smiths Fork Vegetation Restoration Project published... an objection under the Healthy Forests Restoration Act. The opportunity to provide scoping...

  6. Estimated Loads of Suspended Sediment and Selected Trace Elements Transported through the Milltown Reservoir Project Area Before and After the Breaching of Milltown Dam in the Upper Clark Fork Basin, Montana, Water Year 2008

    USGS Publications Warehouse

    Lambing, John H.; Sando, Steven K.

    2009-01-01

    This report presents estimated daily and cumulative loads of suspended sediment and selected trace elements transported during water year 2008 at three streamflow-gaging stations that bracket the Milltown Reservoir project area in the upper Clark Fork basin of western Montana. Milltown Reservoir is a National Priorities List Superfund site where sediments enriched in trace elements from historical mining and ore processing have been deposited since the construction of Milltown Dam in 1907. Milltown Dam was breached on March 28, 2008, as part of Superfund remedial activities to remove the dam and contaminated sediment that had accumulated in Milltown Reservoir. The estimated loads transported through the project area during the periods before and after the breaching of Milltown Dam, and for the entire water year 2008, were used to quantify the net gain or loss (mass balance) of suspended sediment and trace elements within the project area during the transition from a reservoir environment to a free-flowing river. This study was done in cooperation with the U.S. Environmental Protection Agency. Streamflow during water year 2008 compared to long-term streamflow, as represented by the record for Clark Fork above Missoula (water years 1930-2008), generally was below normal (long-term median) from about October 2007 through April 2008. Sustained runoff started in mid-April, which increased flows to near normal by mid-May. After mid-May, flows sharply increased to above normal, reaching a maximum daily mean streamflow of 16,800 cubic feet per second (ft3/s) on May 21, which essentially equaled the long-term 10th-exceedance percentile for that date. Flows substantially above normal were sustained through June, then decreased through the summer and reached near-normal by August. Annual mean streamflow during water year 2008 (3,040 ft3/s) was 105 percent of the long-term mean annual streamflow (2,900 ft3/s). The annual peak flow (17,500 ft3/s) occurred on May 21 and was 112

  7. Crafts, Boys, Ernest Thompson Seton, and the Woodcraft Movement

    ERIC Educational Resources Information Center

    Chalmers, F. Graeme; Dancer, Andrea A.

    2008-01-01

    This article examines early influences on art education for boys (Chalmers & Dancer, 2007) in areas traditionally labeled as crafts. Under review is the work of Ernest Thompson Seton, artist, naturalist, storyteller, author, philosopher, crusader for and supporter of indigenous American Indian ways of knowing, and a co-founder of the Boy Scouts of…

  8. 2. Historic American Building Survey History of Sacramento County Thompson ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Building Survey History of Sacramento County Thompson & West Illustration Original 1880 Rephoto 1960 N.W. Corner of 3rd + P ST., SOUTH HOUSE (Property of Mrs. E.B. Crocker, 3rd & P St.) - Crocker Art Gallery, 216 O Street, Sacramento, Sacramento County, CA

  9. 4. Photocopy of lithograph (from Thompson and West, Historical Atlas ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photocopy of lithograph (from Thompson and West, Historical Atlas of Solano County, San Francisco, 1878, p. 52) Lithographer unknown 1878 ST. AUGUSTINE'S COLLEGE, SHOWING SOUTH AND EAST SIDES OF WINGFIELD HOUSE - Bishop J. H. D. Wingfield House, 36 Wingfield Way, Benicia, Solano County, CA

  10. On nonsingular potentials of Cox-Thompson inversion scheme

    SciTech Connect

    Palmai, Tamas; Apagyi, Barnabas

    2010-02-15

    We establish a condition for obtaining nonsingular potentials using the Cox-Thompson inverse scattering method with one phase shift. The anomalous singularities of the potentials are avoided by maintaining unique solutions of the underlying Regge-Newton integral equation for the transformation kernel. As a by-product, new inequality sequences of zeros of Bessel functions are discovered.

  11. Reliability: Rejoinder to Thompson and Vacha-Haase.

    ERIC Educational Resources Information Center

    Sawilowsky, Shlomo S.

    2000-01-01

    B. Thompson and T. Vacha-Haase have examined the statement "the reliability of the test" with emphasis on the following three words: (1) the first "the"; (2) "test"; and (3) the second "the." This discussion focuses instead on the word "reliability." (Author)

  12. Bruce Thompson: Adventures and advances in ultrasonic backscatter

    NASA Astrophysics Data System (ADS)

    Margetan, Frank J.

    2012-05-01

    Over the course of his professional career Dr. R. Bruce Thompson published several hundred articles on non-destructive evaluation, the majority dealing with topics in ultrasonics. One longtime research interest of Dr. Thompson, with applications both to microstructure characterization and defect detection, was backscattered grain noise in metals. Over a 20 year period he led a revolving team of staff members and graduate students investigating various aspects of ultrasonic backscatter. As a member of that team I had the privilege of working along side Dr. Thompson for many years, serving as a sort of Dr. Watson to Bruce's Sherlock Holmes. This article discusses Dr. Thompson's general approaches to modeling backscatter, the research topics he chose to explore to systematically elucidate a better understanding of the phenomena, and the many contributions to the field achieved under his leadership. The backscatter work began in earnest around 1990, motivated by a need to improve inspections of aircraft engine components. At that time Dr. Thompson launched two research efforts. The first led to the heuristic Independent Scatterer Model which could be used to estimate the average grain noise level that would be seen in any given ultrasonic inspection. There the contribution from the microstructure was contained in a measureable parameter known as the Figure-of-Merit or FOM. The second research effort, spearheaded by Dr. Jim Rose, led to a formal relationship between FOM and details of the metal microstructure. The combination of the Independent Scattering Model and Rose's formalism provided a powerful tool for investigating backscatter in metals. In this article model developments are briefly reviewed and several illustrative applications are discussed. These include: the determination of grain size and shape from ultrasonic backscatter; grain noise variability in engine-titanium billets and forgings; and the design of ultrasonic inspection systems to improve defect

  13. Parents'/Carers' Perceptions and Experiences of Growing, Preparing and Eating Their Own Fruit and Vegetables as Part of the "Field to Fork" Project

    ERIC Educational Resources Information Center

    Burton, Diana M.; May, Stephanie

    2016-01-01

    This paper reports research into a project to encourage KS1 and KS2 pupils to eat more healthily by supporting their families to grow their own fruit and vegetables at home. Participants were recruited through a Primary School Trust comprising four primary schools in the North West of England. They were given practical support to enable them to…

  14. M2-F1 on lakebed with pilot Milt Thompson

    NASA Technical Reports Server (NTRS)

    1963-01-01

    adequate for the roughly 400 car tows that got the M2-F1 airborne to prove it could fly safely and to train pilots before they were towed behind a C-47 aircraft and released. These initial car-tow tests produced enough flight data about the M2-F1 to proceed with flights behind the C-47 tow plane at greater altitudes. The C-47 took the craft to an altitude of 12,000 where free flights back to Rogers Dry Lake began. Pilot for the first series of flights of the M2-F1 was NASA research pilot Milt Thompson. Typical glide flights with the M2-F1 lasted about two minutes and reached speeds of 110 to l20 mph. A small solid landing rocket, referred to as the 'instant L/D rocket,' was installed in the rear base of the M2-F1. This rocket, which could be ignited by the pilot, provided about 250 pounds of thrust for about 10 seconds. The rocket could be used to extend the flight time near landing if needed. More than 400 ground tows and 77 aircraft tow flights were carried out with the M2-F1. The success of Dryden's M2-F1 program led to NASA's development and construction of two heavyweight lifting bodies based on studies at NASA's Ames and Langley research centers--the M2-F2 and the HL-10, both built by the Northrop Corporation, and the U.S. Air Force's X-24 program, with an X-24A and -B built by Martin. The Lifting Body program also heavily influenced the Space Shuttle program. The M2-F1 program demonstrated the feasibility of the lifting body concept for horizontal landings of atmospheric entry vehicles. It also demonstrated a procurement and management concept for prototype flight test vehicles that produced rapid results at very low cost (approximately $50,000, excluding salaries of government employees assigned to the project).

  15. 5. View of Clark Fork Vehicle Bridge facing east. Bridge ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View of Clark Fork Vehicle Bridge facing east. Bridge from south shore of Clark Fork River-southernmost span. 1900-era Northern Pacific Railway Bridge in background. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  16. Replication fork collapse at replication terminator sequences.

    PubMed

    Bidnenko, Vladimir; Ehrlich, S Dusko; Michel, Bénédicte

    2002-07-15

    Replication fork arrest is a source of genome re arrangements, and the recombinogenic properties of blocked forks are likely to depend on the cause of blockage. Here we study the fate of replication forks blocked at natural replication arrest sites. For this purpose, Escherichia coli replication terminator sequences Ter were placed at ectopic positions on the bacterial chromosome. The resulting strain requires recombinational repair for viability, but replication forks blocked at Ter are not broken. Linear DNA molecules are formed upon arrival of a second round of replication forks that copy the DNA strands of the first blocked forks to the end. A model that accounts for the requirement for homologous recombination for viability in spite of the lack of chromosome breakage is proposed. This work shows that natural and accidental replication arrests sites are processed differently.

  17. Topological locking restrains replication fork reversal

    PubMed Central

    Fierro-Fernández, Marta; Hernández, Pablo; Krimer, Dora B.; Stasiak, Andrzej; Schvartzman, Jorge B.

    2007-01-01

    Two-dimensional agarose gel electrophoresis, psoralen cross-linking, and electron microscopy were used to study the effects of positive supercoiling on fork reversal in isolated replication intermediates of bacterial DNA plasmids. The results obtained demonstrate that the formation of Holliday-like junctions at both forks of a replication bubble creates a topological constraint that prevents further regression of the forks. We propose that this topological locking of replication intermediates provides a biological safety mechanism that protects DNA molecules against extensive fork reversals. PMID:17242356

  18. Acoustic Doppler current profiling from the JGOFS Arabian Sea cruises aboard the RV T.G. Thompson

    SciTech Connect

    Kim, H.S.; Flagg, C.N.; Shi, Y.

    1996-12-01

    Acoustic Doppler current profiler (ADCP) data is part of the core data for the US JGOFS Arabian Sea project, along with hydrographic and nutrient data. Seventeen cruises are scheduled to take place between September 1994 and January 1996 on the R/V T.G. Thompson. Seven of the cruises follow a standard cruise track, taking hydrographic, chemical and biological measurements. The rest of the cruises, which take place generally within the standard cruise region defined by a set track, are for the deployment and recovery of moored equipment and towing of a SeaSoar. Detailed description of ADCP hardware, the AutoADCP data acquisition system, and the collection of navigation and compass data on the Thompson is documented in Section 2. Followed by data collection for each cruise together with a cruise track, Section 3 presents the processing and analysis of velocity and acoustic backscatter intensity data. Section 5 shows results of profile quality diagnosis.

  19. South Fork Clearwater River Habitat Enhancement, Nez Perce National Forest.

    SciTech Connect

    Siddall, Phoebe

    1992-04-01

    In 1984, the Nez Perce National forest and the Bonneville Power Administration entered into a contractual agreement which provided for improvement of spring chinook salmon and summer steelhead trout habitat in south Fork Clearwater River tributaries. Project work was completed in seven main locations: Crooked River, Red River, Meadow Creek Haysfork Gloryhole, Cal-Idaho Gloryhole, Fisher Placer and Leggett Placer. This report describes restoration activities at each of these sites.

  20. Approach view of the North Fork Butter Creek Bridge, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Approach view of the North Fork Butter Creek Bridge, view looking south - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  1. Elevation view of the North Fork Butter Creek Bridge, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Elevation view of the North Fork Butter Creek Bridge, view looking west - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  2. Detail perspective view of the North Fork Butter Creek Bridge, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail perspective view of the North Fork Butter Creek Bridge, view looking southwest - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  3. General perspective view of the North Fork Butter Creek Bridge, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General perspective view of the North Fork Butter Creek Bridge, view looking north - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  4. General perspective view of the North Fork Butter Creek Bridge, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General perspective view of the North Fork Butter Creek Bridge, view looking south - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  5. General perspective view of the North Fork Butter Creek Bridge, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General perspective view of the North Fork Butter Creek Bridge, view looking southwest - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  6. Approach view of the North Fork Butter Creek Bridge, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Approach view of the North Fork Butter Creek Bridge, view looking north - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  7. Sarcophilia, cremation and Sir Henry Thompson (1820-1904).

    PubMed

    Jellinek, E H

    2009-11-01

    Sarcophilia, a neologism for an attachment to human remains, is set in a review of the history of the disposal of the dead. The ancient practice of cremation was relaunched late in the 19th century by the urological surgeon cum social reformer Sir Henry Thompson. He was stimulated by Edwin Chadwick and Charles Dickens, and by Charles Darwin's observations on the earthworm. Sarcophilia is the reason for the controversial Human Tissue Act of 2004. PMID:20029076

  8. Sarcophilia, cremation and Sir Henry Thompson (1820-1904).

    PubMed

    Jellinek, E H

    2009-11-01

    Sarcophilia, a neologism for an attachment to human remains, is set in a review of the history of the disposal of the dead. The ancient practice of cremation was relaunched late in the 19th century by the urological surgeon cum social reformer Sir Henry Thompson. He was stimulated by Edwin Chadwick and Charles Dickens, and by Charles Darwin's observations on the earthworm. Sarcophilia is the reason for the controversial Human Tissue Act of 2004.

  9. Themes and variation of N. S. Thompson--song.

    PubMed

    Abbey, Emily

    2010-12-01

    Nick Thompson studied many animals over the course of his career, including non-human primates, dogs, crows, human babies, and mockingbirds. Amidst such variation, Nick maintained a common focus. He sought to provide more accurate and truthful representations of the particular phenomenon of interest. His writings on mentalism, design, anthropomorphism and use of metaphor have provided fellow scientists with insight and helped advance his field of study.

  10. 1976 Big Thompson Flood, Colorado - Thirty Years Later

    USGS Publications Warehouse

    Jarrett, Robert D.; Costa, John E.

    2006-01-01

    In the early evening of Saturday, July 31, 1976, a large stationary thunderstorm released as much as 7.5 inches of rainfall in about an hour (about 12 inches in a few hours) in the middle reaches of the Big Thompson River Basin and to a lesser extent in parts of the Cache la Poudre River Basin. In steep mountain terrain with thin or no soil, this large amount of rainfall in such a short period of time produced a flash flood that caught residents and tourists by surprise. The sudden flood that churned down the narrow Big Thompson Canyon scoured the river channel that night, caused over $35 million in damages (1977 dollars) to 418 homes and businesses, many mobile homes, 438 automobiles, numerous bridges, paved and unpaved roads, power and telephone lines, and many other structures. The tragedy claimed the lives of 144 people, including two law enforcement officers trying to evacuate people in danger, and there were 250 reported injuries. Scores of other people narrowly escaped with their lives. More than 800 people were evacuated by helicopter the following morning. This fact sheet presents a summary of the hydrologic conditions of the 1976 flood, describes some of the advances in U.S. Geological Survey (USGS) flood science as a consequence of this disaster, and provides a reminder that extreme floods like the 1976 Big Thompson flood have occurred in other locations in Colorado in the past and will occur again.

  11. Soil Data from a Moderately Well and Somewhat Poorly Drained Fire Chronosequence near Thompson, Manitoba, Canada

    USGS Publications Warehouse

    Manies, K.L.; Harden, J.W.; Veldhuis, Hugo; Trumbore, Sue

    2006-01-01

    The U.S. Geological Survey project Fate of Carbon in Alaskan Landscapes (FOCAL) is studying the effect of fire and soil drainage on soil carbon storage in the boreal forest. As such this group was invited to be a part of a NSF-funded project (Fire, Ecosystem and Succession - Experiment Boreal or FIRES-ExB) to study the carbon balance of sites that varied in age (time since fire) and soil drainage in the Thompson, Manitoba, Canada region. This report describes the location of our FIRES-ExB sampling sites as well as the procedures used to describe, sample, and analyze the soils. This report also contains data tables with sample related information including, but not limited to, field descriptions, bulk density, particle size distribution, moisture content, carbon (C) concentration, nitrogen (N) concentration, isotopic data for C, and major, minor and trace elemental concentration.

  12. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  13. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  14. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  15. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  16. 21 CFR 882.1525 - Tuning fork.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Tuning fork. 882.1525 Section 882.1525 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES NEUROLOGICAL DEVICES Neurological Diagnostic Devices § 882.1525 Tuning fork. (a) Identification. A tuning...

  17. Training Guidelines: Fork Lift Truck Driving.

    ERIC Educational Resources Information Center

    Ceramics, Glass, and Mineral Products Industry Training Board, Harrow (England).

    This manual of operative training guidelines for fork lift truck driving has been developed by the Ceramics, Glass and Mineral Products Industry Training Board (Great Britain) in consultation with a number of firms which manufacture fork lift trucks or which already have training--programs for their use. The purpose of the guidelines is to assist…

  18. Fifth-wheel fork truck adapter

    NASA Technical Reports Server (NTRS)

    Smith, P. L.

    1969-01-01

    Standard fifth wheel mounted on a rectangular steel structure adapted for use with a fork lift truck provides a fast, safe, and economical way of maneuvering semitrailers in close quarters at plants and warehouses. One operator can move and locate a semitrailer without dismounting from a fork lift truck.

  19. Acoustic doppler current profiling from the JGOFS Arabian Sea cruises aboard the RV T.G. Thompson

    SciTech Connect

    Flagg, C.N.; Shi, Y.

    1995-04-01

    Acoustic Doppler Current Profiler (ADCP) data from the R/V T.G. THOMPSON is part of the core data for the US JGOFS Arabian Sea project along with hydrographic and nutrient data. Seventeen cruises on the THOMPSON are scheduled to take place between September 1994 and January 1996. The first of these cruises, a transit of the R/V THOMPSON into the northern Arabian Sea area from Singapore, was a calibration and training cruise that took place between September 18 and October 7, 1994. (The cruises on the THOMPSON are numbered consecutively from the ship`s commissioning with the first JOGFS cruise designated TN039.) The remaining cruises have been and will be staged from Muscat, Oman. Seven of these cruises, referred to as process cruises, will follow a set cruise track, making hydrographic, chemical and biological measurements. The remainder of the cruises while not restricted to the set cruise track, will generally stay within the region defined by the track during the deployment and retrieval of moored equipment and the towing of a SeaSoar. Each cruise will last between two weeks and one month. ADCP data will be collected on all the JGOFS Arabian Sea cruises using an autonomous data acquisition system developed for ship-of-opportunity cruises. This system, referred to as the AutoADCP, makes it possible to collect the ADCP data without the constant monitoring usually necessary and assures constant data coverage and uniform data quality. The AutoADCP system is an extension of RD Instrument`s DAS version 2.48 using enhancements made possible with ``user exit`` programs. This data report presents ADCP results from the first four JGOFS cruises, TN039 through TN042, concentrating on the data collection and processing methods.

  20. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Requirements for front fork. 1512.13 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  1. 23. View of Clark Fork Vehicle Bridge facing upwest side. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. View of Clark Fork Vehicle Bridge facing up-west side. Looking at structural connection of top chord, vertical laced channel and diagonal bars. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  2. 24. View of one of the plaques from Clark Fork ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. View of one of the plaques from Clark Fork Vehicle Bridge. Presently located at the Bonner County Historical Museum in Sandpoint, Idaho. A plaque was attached at each end of the bridge. Only one remains. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  3. 13. View of Clark Fork Vehicle Bridge facing south. Concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. View of Clark Fork Vehicle Bridge facing south. Concrete barrier blocks access. Plaque was originally located where strioed traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  4. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Requirements for front fork. 1512.13 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  5. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Requirements for front fork. 1512.13 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  6. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for front fork. 1512.13 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  7. 14. View of Clark Fork Vehicle Bridge facing north. Approach ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. View of Clark Fork Vehicle Bridge facing north. Approach from the south. Concrete barrier blocks access. Plaque was originally located where striped traffic sign is posted at right. - Clark Fork Vehicle Bridge, Spanning Clark Fork River, serves Highway 200, Clark Fork, Bonner County, ID

  8. 16 CFR 1512.13 - Requirements for front fork.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Requirements for front fork. 1512.13 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.13 Requirements for front fork. The front fork shall... fork test, § 1512.18(k)(1), without visible evidence of fracture. Sidewalk bicycles need not meet...

  9. South Fork Holston River basin 1988 biomonitoring

    SciTech Connect

    Saylor, C.F.; Ahlstedt, S.A.

    1990-06-01

    There is concern over the effects of shifts in land use use practices on the aquatic fauna of streams in the South Fork Holston River basin in northwestern North Carolina and southwestern Virginia. Trout reproduction has noticeably declined in the Watauga River subbasin. The Watauga River and Elk River subbasins have been subjected to commercial and resort development. The Middle fork Holston River and the upper South Fork Holston River subbasins have been affected by agricultural and mining activities, respectively (Cox, 1986). To aid reclamation and management of the South Fork Holston basin, Tennessee Valley Authority (TVA) biologists conducted biomonitoring--including index of biotic integrity and macroinvertebrate sampling--on the Middle Fork Holston, South Fork Holston, Watauga, and Elk Rivers to assess cumulative impairment related to changes in habitat and pollutant loading in these subbasins. Biomonitoring can detect environmental degradation, help document problem areas, and assist in development of strategies for managing water quality. This report discusses the methods and materials and results of the biomonitoring of South Fork Holston River Basin. 13 refs., 5 figs., 12 tabs.

  10. Yawning, fatigue, and cortisol: expanding the Thompson Cortisol Hypothesis.

    PubMed

    Thompson, Simon B N

    2014-10-01

    Yawning and its involvement in neurological disorders has become the new scientific conundrum. Cortisol levels are known to rise during stress and fatigue; yawning may occur when we are under stress or tired. However, the link between yawning, fatigue, and cortisol has not been fully understood. Expansion of the Thompson Cortisol Hypothesis proposes that the stress hormone, cortisol, is responsible for yawning and fatigue especially in people with incomplete innervation such as multiple sclerosis. This informs our understanding of the functional importance of the brain stem region of the brain in regulating stress and fatigue.

  11. Yawning, fatigue, and cortisol: expanding the Thompson Cortisol Hypothesis.

    PubMed

    Thompson, Simon B N

    2014-10-01

    Yawning and its involvement in neurological disorders has become the new scientific conundrum. Cortisol levels are known to rise during stress and fatigue; yawning may occur when we are under stress or tired. However, the link between yawning, fatigue, and cortisol has not been fully understood. Expansion of the Thompson Cortisol Hypothesis proposes that the stress hormone, cortisol, is responsible for yawning and fatigue especially in people with incomplete innervation such as multiple sclerosis. This informs our understanding of the functional importance of the brain stem region of the brain in regulating stress and fatigue. PMID:25169036

  12. The use of Thompson sampling to increase estimation precision.

    PubMed

    Kaptein, Maurits

    2015-06-01

    In this article, we consider a sequential sampling scheme for efficient estimation of the difference between the means of two independent treatments when the population variances are unequal across groups. The sampling scheme proposed is based on a solution to bandit problems called Thompson sampling. While this approach is most often used to maximize the cumulative payoff over competing treatments, we show that the same method can also be used to balance exploration and exploitation when the aim of the experimenter is to efficiently increase estimation precision. We introduce this novel design optimization method and, by simulation, show its effectiveness.

  13. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS, AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    EPA Science Inventory

    This technical report provides a description of the field project design, quality control, the sampling protocols and analysis methodology used, and standard operating procedures for the South Fork Broad River Watershed (SFBR) Total Maximum Daily Load (TMDL) project. This watersh...

  14. WRNIP1 protects stalled forks from degradation and promotes fork restart after replication stress.

    PubMed

    Leuzzi, Giuseppe; Marabitti, Veronica; Pichierri, Pietro; Franchitto, Annapaola

    2016-07-01

    Accurate handling of stalled replication forks is crucial for the maintenance of genome stability. RAD51 defends stalled replication forks from nucleolytic attack, which otherwise can threaten genome stability. However, the identity of other factors that can collaborate with RAD51 in this task is poorly elucidated. Here, we establish that human Werner helicase interacting protein 1 (WRNIP1) is localized to stalled replication forks and cooperates with RAD51 to safeguard fork integrity. We show that WRNIP1 is directly involved in preventing uncontrolled MRE11-mediated degradation of stalled replication forks by promoting RAD51 stabilization on ssDNA We further demonstrate that replication fork protection does not require the ATPase activity of WRNIP1 that is however essential to achieve the recovery of perturbed replication forks. Loss of WRNIP1 or its catalytic activity causes extensive DNA damage and chromosomal aberrations. Intriguingly, downregulation of the anti-recombinase FBH1 can compensate for loss of WRNIP1 activity, since it attenuates replication fork degradation and chromosomal aberrations in WRNIP1-deficient cells. Therefore, these findings unveil a unique role for WRNIP1 as a replication fork-protective factor in maintaining genome stability.

  15. South Fork Clearwater River Habitat Enhancement, Crooked and Red Rivers : Annual Report, 1989.

    SciTech Connect

    Baer, William H.

    1990-01-01

    In 1983, the Nez Perce National Forest and the Bonneville Power Administration entered into an interagency agreement to enhance and improve habitat for two anadromous fish species, spring chinook salmon (Oncorhynchus tshawyscha) and summer steelhead trout (Onchorhyncus mykiss), in the South Fork Clearwater River tributaries. The South Fork Clearwater River was dammed in 1927 for hydroelectric development. Anadromous fish runs were virtually eliminated until the dam was removed in 1962. To complicate the problem, upstream spawning and rearing habitats were severely impacted by dredge and hydraulic mining, road building, timber harvest, and over-grazing. Fish habitat improvement projects under the above contract are being carried out in two major tributaries to the South Fork Clearwater River. Both the Red River and the Crooked River projects began in 1983 and will be completed in 1990. 12 figures., 1 tab.

  16. Storm and flood of July 31-August 1, 1976, in the Big Thompson River and Cache la Poudre River basins, Larimer and Weld Counties, Colorado

    USGS Publications Warehouse

    McCain, Jerald F.; Shroba, R.R.

    1979-01-01

    PART A: Devastating flash floods swept through the canyon section of Larimer County in north-central Colorado during the night of July 31-August I, 1976, causing 139 deaths, 5 missing persons, and more than $35 million in total damages. The brunt of the storms occurred over the Big Thompson River basin between Drake and Estes Park with rainfall amounts as much as 12 inches being reported during the storm period. In the Cache la Poudre River basin to the north, a rainfall amount of 10 inches was reported for one locality while 6 inches fell over a widespread area near the central part of the basin. The storms developed when strong low-level easterly winds to the rear of a polar front pushed a moist, conditionally unstable airmass upslope into the Front Range of the Rocky Mountains. Orographic uplift released the convective instability, and light south-southeasterly winds at middle and upper levels allowed the storm complex to remain nearly stationary over the foothills for several hours. Minimal entrainment of relatively moist air at middle and upper levels, very low cloud bases, and a slightly tilted updraft structure contributed to a high precipitation efficiency. Intense rainfall began soon after 1900 MDT (Mountain Daylight Time) in the Big Thompson River and the North Fork Cache la Poudre River basins. A cumulative rainfall curve developed for Glen Comfort from radar data indicates that 7.5 inches of rain fell during the period 1930-2040 MDT on July 31. In the central part of the storm area west of Fort Collins, the heaviest rainfall began about 2200 MDT on July 31 and continued until 0100 MDT on August 1. Peak discharges were extremely large on many streams in the storm area-exceeding previously recorded maximum discharges at several locations. The peak discharge of the Big Thompson River at the gaging station at the canyon mouth, near Drake was 31,200 cubic feet per second or more than four times the previous maximum discharge of 7,600 cubic feet per second at

  17. Biochemical characteristics and thermal inhibition kinetics of polyphenol oxidase extracted from Thompson seedless grape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Polyphenol oxidase (PPO) was isolated from Thompson seedless grape (Vitis vinifera 'Thompson Seedless') and its biochemical characteristics were studied. Optimum pH and temperature for grape PPO activity were pH 6.0 and 25 degrees C with 10 mM catechol as substrate. The enzyme was heat-stable betwee...

  18. Revisiting Charles H. Thompson's Proposals for Educating Gifted African American Students, 1933-1961

    ERIC Educational Resources Information Center

    Ray, Louis

    2012-01-01

    Charles H. Thompson is best known as the founder and the first editor-in-chief of "The Journal of Negro Education" (1932-1963). Throughout his career, Thompson sought to extend educational opportunity in ways that were "for the good of Negro education as a whole." His main concern was in educating future leaders for service in African American…

  19. Water quality and trend analysis of Colorado--Big Thompson system reservoirs and related conveyances, 1969 through 2000

    USGS Publications Warehouse

    Stevens, Michael R.

    2003-01-01

    The U.S. Geological Survey, in an ongoing cooperative monitoring program with the Northern Colorado Water Conservancy District, Bureau of Reclamation, and City of Fort Collins, has collected water-quality data in north-central Colorado since 1969 in reservoirs and conveyances, such as canals and tunnels, related to the Colorado?Big Thompson Project, a water-storage, collection, and distribution system. Ongoing changes in water use among agricultural and municipal users on the eastern slope of the Rocky Mountains in Colorado, changing land use in reservoir watersheds, and other water-quality issues among Northern Colorado Water Conservancy District customers necessitated a reexamination of water-quality trends in the Colorado?Big Thompson system reservoirs and related conveyances. The sampling sites are on reservoirs, canals, and tunnels in the headwaters of the Colorado River (on the western side of the transcontinental diversion operations) and the headwaters of the Big Thompson River (on the eastern side of the transcontinental diversion operations). Carter Lake Reservoir and Horsetooth Reservoir are off-channel water-storage facilities, located in the foothills of the northern Colorado Front Range, for water supplied from the Colorado?Big Thompson Project. The length of water-quality record ranges from approximately 3 to 30 years depending on the site and the type of measurement or constituent. Changes in sampling frequency, analytical methods, and minimum reporting limits have occurred repeatedly over the period of record. The objective of this report was to complete a retrospective water-quality and trend analysis of reservoir profiles, nutrients, major ions, selected trace elements, chlorophyll-a, and hypolimnetic oxygen data from 1969 through 2000 in Lake Granby, Shadow Mountain Lake, and the Granby Pump Canal in Grand County, Colorado, and Horsetooth Reservoir, Carter Lake, Lake Estes, Alva B. Adams Tunnel, and Olympus Tunnel in Larimer County, Colorado

  20. Transonic Flows of Bethe-Zel'dovich-Thompson Fluids

    NASA Astrophysics Data System (ADS)

    Cramer, Mark; Andreyev, Aleksandr

    2013-11-01

    We examine steady transonic flows of Bethe-Zel'dovich-Thompson (BZT) fluids over thin turbine blades or airfoils. BZT fluids are ordinary fluids having a region of negative fundamental derivative over a finite range of pressures and temperatures in the single phase regime. We present the transonic small disturbance equation, shock jump conditions, and shock existence conditions capable of capturing the qualitative behavior of BZT fluids. The flux function is seen to be quartic in the pressure or density perturbation rather than the quadratic (convex) flux function of the perfect gas theory. We show how this nonconvex flux function can be used to predict and explain the complex flows possible. Numerical solutions using a successive line relaxation (SLR) scheme are presented. New results of interest include shock-splitting, collisions between expansion and compression shocks, two compressive bow shocks in supersonic flows, and the observation of as many as three normal stern shocks following an oblique trailing edge shock.

  1. Thompson Receives 2013 Early Career Hydrologic Science Award: Citation

    NASA Astrophysics Data System (ADS)

    Katul, Gabriel

    2014-08-01

    Sally Thompson grew up in Perth, where she was trained as an environmental engineer at the University of Western Australia. She graduated with honors in 2003 and worked for a few years as an environmental engineering consultant. Following the award of the Sir John Monash Fellowship in Australia, Sal accepted the admissions offer from the Nicholas School of the Environment at Duke University in 2006, completing her Ph.D. within 4 years and defending her dissertation in 2010. I was most fortunate to have Sal join me at Duke after an enthusiastic recommendation from Siva. Upon her arrival at Duke University, it was immediately clear to all that Sal is a special person with the remarkable skill of being able to identify the main aspects of a problem and throw at them the best that theory, experiment, and modeling tools offer.

  2. Hip hemiarthroplasty: from Venable and Bohlman to Moore and Thompson.

    PubMed

    Hernigou, Philippe; Quiennec, Steffen; Guissou, Isaac

    2014-03-01

    In 1939, Frederick R. Thompson of New York and Austin T. Moore of South Carolina separately developed replacements for the entire ball of the hip. These were used to treat hip fractures and also certain arthritis cases. This type of hemiarthroplasty addressed the problem of the arthritic femoral head only. The diseased acetabulum (hip socket) was not replaced. This prosthesis consisted of a metal stem that was placed into the marrow cavity of the femur, connected in one piece with a metal ball fitted into the hip socket. Bohlman and Austin T. Moore (1939) collaborated for the fabrication and implantation of a custom made 12-inch-long vitallium (metal alloy invented by Venable) femoral head prosthesis for a patient with a recurrent giant cell tumour. This prosthesis functioned well and later on influenced the development of long stem femoral head prostheses.

  3. 75 FR 25197 - Shasta Trinity National Forest, South Fork Management Unit, California Salt Timber Harvest and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Shasta Trinity National Forest, South Fork Management Unit, California Salt Timber Harvest and Fuels Hazard Reduction Project AGENCY: USDA Forest Service. ACTION: Notice of intent to prepare...

  4. Storm water control plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    SciTech Connect

    1996-04-01

    This document provides the Environmental Restoration Program with information about the erosion and sediment control, storm water management, maintenance, and reporting and record keeping practices to be employed during Phase II of the remediation project for the Lower East Fork Poplar Creek (LEFPC) Operable Unit.

  5. Tuning fork shear-force feedback.

    PubMed

    Ruiter, A G; van der Werf, K O; Veerman, J A; Garcia-Parajo, M F; Rensen, W H; van Hulst, N F

    1998-03-01

    Investigations have been performed on the dynamics of a distance regulation system based on an oscillating probe at resonance. This was examined at a tuning fork shear-force feedback system, which is used as a distance control mechanism in near-field scanning optical microscopy. In this form of microscopy, a tapered optical fiber is attached to the tuning fork and scanned over the sample surface to be imaged. Experiments were performed measuring both amplitude and phase of the oscillation of the tuning fork as a function of driving frequency and tip-sample distance. These experiments reveal that the resonance frequency of the tuning fork changes upon approaching the sample. Both the amplitude and the phase of the tuning fork can be used as distance control parameter in the feedback system. Using the amplitude a second-order behavior is observed, while with phase only a first-order behavior is observed. Numerical calculations confirm these observations. This first-order behavior results in an improved stability of the feedback system. As an example, a sample consisting of DNA strands on mica was imaged which showed the height of the DNA as 1.4 +/- 0.2 nm.

  6. South Fork Latrine showing north and west sides, general view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine showing north and west sides, general view to southeast - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  7. South Fork Latrine, oblique view showing south and east sides; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, oblique view showing south and east sides; view northwest - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  8. South Fork Latrine, east elevation showing structure in context, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, east elevation showing structure in context, view west - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  9. 12. Roaring Fork Motor Nature Trail, place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Roaring Fork Motor Nature Trail, place of a thousand drips, view from road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  10. 9. Roaring Fork Motor Nature Trail, Reagan House. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roaring Fork Motor Nature Trail, Reagan House. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  11. 11. Roaring Fork Motor Nature Trail, boulders along road after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Roaring Fork Motor Nature Trail, boulders along road after stop 13. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  12. Roaring Fork Motor Nature Trail, Title Sheet Great Smoky ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Roaring Fork Motor Nature Trail, Title Sheet - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  13. 6. Roaring Fork Motor Nature Trail, road view after stop ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Roaring Fork Motor Nature Trail, road view after stop four. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  14. 7. Roaring Fork Motor Nature Trail, rocks along edge of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Roaring Fork Motor Nature Trail, rocks along edge of road. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  15. 3. Roaring Fork Motor Nature Trail, view between second and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Roaring Fork Motor Nature Trail, view between second and third stops - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  16. 5. Roaring Fork Motor Nature Trail, vista at stop three. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Roaring Fork Motor Nature Trail, vista at stop three. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  17. 8. Roaring Fork Motor Nature Trail, handbuilt rock pile. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Roaring Fork Motor Nature Trail, hand-built rock pile. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  18. 1. Roaring Fork Motor Nature Trail, entrance sign. Great ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Roaring Fork Motor Nature Trail, entrance sign. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. 2. Roaring Fork Motor Nature Trail, road view before first ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Roaring Fork Motor Nature Trail, road view before first stop. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  20. Resonant tuning fork detector for electromagnetic radiation.

    PubMed

    Pohlkötter, Andreas; Willer, Ulrike; Bauer, Christoph; Schade, Wolfgang

    2009-02-01

    A mechanical quartz microresonator (tuning fork) is used to detect electromagnetic radiation. The detection scheme is based on forces created due to the incident electromagnetic radiation on the piezoelectric tuning fork. A force can be created due to the transfer of the photon momentum of the incident electromagnetic radiation. If the surfaces of the tuning fork are nonuniformly heated, a second force acts on it, the so-called photophoretic force. These processes occur for all wavelengths of the incident radiation, making the detector suitable for sensing of ultraviolet, visible, and mid-infrared light, even THz-radiation. Here the detector is characterized in the visible range; noise analysis is performed for 650 nm and 5.26 microm. A linear power characteristic and the dependence on pulse lengths of the incoming light are shown. Examples for applications for the visible and mid-infrared spectral region are given by 2f and absorption spectroscopy of oxygen and nitric oxide, respectively.

  1. Yielding the bully pulpit: a conversation with Tommy Thompson. Interview by John K Iglehart.

    PubMed

    Thompson, Tommy

    2005-01-01

    In January 2005 Tommy Thompson relinquished the post of secretary of the U.S. Department of Human Services (HHS). Known as being an outspoken defender of his principles throughout his tenure at HHS, Thompson continues that tone in an interview conducted shortly after he left Washington. Topics covered include ways to curb Medicare spending; devote greater attention to preventive health and proper incentives for technology; improve the vaccine infrastructure of the United States and the world; and continue to infuse U.S. foreign policy with what Thompson has termed "medical diplomacy," in an effort to counter the messages of America's enemies.

  2. The Little Thompson Observatory Receives the Retired Mt. Wilson 24-inch Telescope

    NASA Astrophysics Data System (ADS)

    Schweitzer, Andrea E.

    2006-12-01

    The Little Thompson Observatory is a community-built E/PO observatory and is a member of the Telescopes in Education (TIE) project. The observatory is located on the grounds of Berthoud High School in northern Colorado. Annually we have approximately 5,000 visitors, which is roughly equal to the population of the small town of Berthoud. During 2006 the LTO received the recently-retired 24-inch telescope from Mount Wilson. To provide a new home for this historic telescope, we are building a second dome and doubling the size of our observatory (all with volunteer labor), as well as refurbishing the telescope. This past year we held a Colorado Project ASTRO-GEO workshop, and used the funding from our NASA ROSS E/PO grant for additional teacher workshops -making use of the baseball-sized meteorite that landed in Berthoud in 2004. The observatory also supported magnet high-school astronomy courses available to students from the surrounding school districts. We thank the NASA ROSS E/PO program for providing funding.

  3. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Roanoke. 9... North Fork of Roanoke. (a) Name. The name of the viticultural area described in this section is “North Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the...

  4. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false North Fork of Roanoke. 9... North Fork of Roanoke. (a) Name. The name of the viticultural area described in this section is “North Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the...

  5. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false North Fork of Roanoke. 9... North Fork of Roanoke. (a) Name. The name of the viticultural area described in this section is “North Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the...

  6. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false North Fork of Roanoke. 9... North Fork of Roanoke. (a) Name. The name of the viticultural area described in this section is “North Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the...

  7. Pyrimidine dimers block simian virus 40 replication forks

    SciTech Connect

    Berger, C.A.; Edenberg, H.J.

    1986-10-01

    UV light produces lesions, predominantly pyrimidine dimers, which inhibit DNA replication in mammalian cells. The mechanism of inhibition is controversial: is synthesis of a daughter strand halted at a lesion while the replication fork moves on and reinitiates downstream, or is fork progression itself blocked for some time at the site of a lesion. We directly addressed this question by using electron microscopy to examine the distances of replication forks from the origin in unirradiated and UV-irradiated simian virus 40 chromosomes. If UV lesions block replication fork progression, the forks should be asymmetrically located in a large fraction of the irradiated molecules; if replication forks move rapidly past lesions, the forks should be symmetrically located. A large fraction of the simian virus 40 replication forks in irradiated molecules were asymmetrically located, demonstrating that UV lesions present at the frequency of pyrimidine dimers block replication forks. As a mechanism for this fork blockage, we propose that polymerization of the leading strand makes a significant contribution to the energetics of fork movement, so any lesion in the template for the leading strand which blocks polymerization should also block fork movement.

  8. 27 CFR 9.65 - North Fork of Roanoke.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false North Fork of Roanoke. 9... North Fork of Roanoke. (a) Name. The name of the viticultural area described in this section is “North Fork of Roanoke.” (b) Approved maps. The appropriate maps for determining the boundaries of the...

  9. CLEAR FORK OF THE BRAZOS SUSPENSION BRIDGE, CIRCA 1896, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CLEAR FORK OF THE BRAZOS SUSPENSION BRIDGE, CIRCA 1896, SHOWING INCLINED STAY CABLES EXTENDING FROM TOP OF TOWER TO DECK. 3/4 VIEW FROM BELOW. - Clear Fork of Brazos River Suspension Bridge, Spanning Clear Fork of Brazos River at County Route 179, Albany, Shackelford County, TX

  10. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  11. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  12. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  13. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  14. 27 CFR 9.113 - North Fork of Long Island.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false North Fork of Long Island... North Fork of Long Island. (a) Name. The name of the viticultural area described in this section is “North Fork of Long Island.” (b) Approved maps. The appropriate maps for determining the boundaries...

  15. Topographic view of the North Fork Butter Creek Bridge (located ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Topographic view of the North Fork Butter Creek Bridge (located center of frame), view looking west - North Fork Butter Creek Bridge, Spanning North Fork Butter Creek Bridge at Milepost 76.63 on Heppner Highway (Oregon Route 74), Pilot Rock, Umatilla County, OR

  16. Wildlife Impact Assessment and Summary of Previous Mitigation Related to Hydroelectric Projects in Montana, Phase 1, Volume Two (B), Clark Fork River Projects, Cabinet Gorge and Noxon Rapids Dams, Operator, Washington Water Power Company.

    SciTech Connect

    Wood, Marilyn

    1984-06-01

    This report documents best available information concerning the wildlife species impacted and the degree of the impact. A target species list was developed to focus the impact assessment and to direct mitigation efforts. Many non-target species also incurred impacts but are not discussed in this report. All wildlife habitats inundated by the two reservoirs are represented by the target species. It was assumed the numerous non-target species also affected will be benefited by the mitigation measures adopted for the target species. Impacts addressed are limited to those directly attributable to the loss of habitat and displacement of wildlife populations due to the construction and operation of the two hydroelectric projects. Secondary impacts, such as the relocation of railroads and highways, and the increase of the human population, were not considered. In some cases, both positive and negative impacts were assessed; and the overall net effect was reported. The loss/gain estimates reported represent impacts considered to have occurred during one point in time except where otherwise noted. When possible, quantitative estimates were developed based on historical information from the area or on data from similar areas. Qualitative loss estimates of low, moderate, or high with supporting rationale were assessed for each species or species group.

  17. The Golden-Thompson inequality: Historical aspects and random matrix applications

    SciTech Connect

    Forrester, Peter J. Thompson, Colin J.

    2014-02-15

    The Golden-Thompson inequality, Tr (e{sup A+B}) ⩽ Tr (e{sup A}e{sup B}) for A, B Hermitian matrices, appeared in independent works by Golden and Thompson published in 1965. Both of these were motivated by considerations in statistical mechanics. In recent years the Golden-Thompson inequality has found applications to random matrix theory. In this article, we detail some historical aspects relating to Thompson's work, giving in particular a hitherto unpublished proof due to Dyson, and correspondence with Pólya. We show too how the 2 × 2 case relates to hyperbolic geometry, and how the original inequality holds true with the trace operation replaced by any unitarily invariant norm. In relation to the random matrix applications, we review its use in the derivation of concentration type lemmas for sums of random matrices due to Ahlswede-Winter, and Oliveira, generalizing various classical results.

  18. Dioxin Registry report for Thompson Chemical Company, St. Louis, Missouri

    SciTech Connect

    Marlow, D.A.; Fingerhut, M.A.

    1991-01-01

    An industrial hygiene survey was conducted at the Thompson Chemical Company, St. Louis, Missouri. The company manufactured sodium-2,4,5-trichlorophenate (NaTCP), 2,4,5-trichlorophenoxyacetic-acid (2,4,5-T), and esters and amines of 2,4,5-T from 1949 through 1970. The company manufactured Agent-Orange for the Air Force from 1967 until 1969. The facility, its processes, its workforce, and 2,3,7,8-tetrachlorodibenzo-p-dioxin (1746016) (TCDD) analytical results for these products were examined. As this firm filed for bankruptcy in 1970, there was only limited data available. The facility employed about 12 persons year round and about double this number during the busy season and during the Agent-Orange contract. Almost no information was available about the production processes. Nine persons developed chloracne after an accident and fire in the NaTCP process. Few personnel records were available. The authors conclude that workers employed from 1949 through 1970 are suitable for inclusion into the Dioxin Registry. There is not enough information available to construct a TCDD exposure matrix for this site.

  19. Canup, Sigman, and Thompson Receive 2004 James B. Macelwane Medal

    NASA Astrophysics Data System (ADS)

    Halliday, Alexander; Bender, Michael L.; Randall, David A.

    2005-01-01

    Robin M. Canup, Daniel Sigman, and David W. J. Thompson received the Macelwane Medal at the 2004 Fall Meeting Honors Ceremony on 15 December, in San Francisco, California. The medal is given for significant contributions to the geophysical sciences by a young scientist of outstanding ability. Citation for Robin M. Canup. It is a great honor to introduce Robin Canup as a recipient of this distinguished and richly deserved award. Robin studies the formation of planets and their satellites-a subject of intrinsic significance and current fascination. As astrophysicists accumulate evidence of planets around other stars, the question of how to make a solar system like our own has yet to be answered. This tough and complex issue has been tackled by geochemists, but just as importantly by a select number of theoretical astrophysicists who deduce the dynamics of accretion. Robin is a relatively new player in this elite group, but her work has put her at the forefront. There are several important contributions one could highlight.

  20. Development of the Pintle Release Fork Mechanism

    SciTech Connect

    BOGER, R.M.; DALE, R.

    1999-08-27

    An improved method of attachment of the pintle to the piston in the universal sampler is being developed. The mechanism utilizes a forked release disk which captures two balls in a cavity formed by a hole in the piston and a groove in the pintle rod.

  1. Man-induced gradient adjustment of the South Fork Forked Deer River, west Tennessee

    USGS Publications Warehouse

    Simon, A.; Robbins, C.H.

    1987-01-01

    Channel modifications from 1968 to 1969 on the South Fork Forked Deer River in western Tennessee have caused upstream degradation, downstream aggradation, and bank failures along the altered channels, adjacent reaches, and tributaries. The result of these adjustments is a general decrease in gradient as the channel attempts to absorb the imposed increase in energy conditions created by channelization. Headward degradation at a rate of approximately 2.57 km/yr on the South Fork Forked Deer River caused from 1.52 m to about 3.14 m of incision over a 13.5 km reach from 1969 to 1981. As a consequence of substantially increased sediment supply, approximately 2.13 m of aggradation was induced downstream of this reach during the same period. This accumulation represents a 60% recovery of bed level at the downstream site since the completion of channel work in 1969. Gradient adjustment with time is described by exponential decay functions. The length of time required for adjustment to some new quasi-equilibrium condition is computed by these decay functions and is about 20 years from the completion of channel work. Adjusted slopes are less than predisturbed values, probably because straightened channels dissipate less energy by friction, allowing more energy for sediment transport. An equivalent sediment load, therefore, can be transported at a considerably gentler slope. The predisturbed slope exceeds the adjusted slope by an order of magnitude on the downstream reach of the South Fork Forked Deer River. ?? 1987 Springer-Verlag New York Inc.

  2. Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks.

    PubMed

    Lecointe, François; Sérèna, Céline; Velten, Marion; Costes, Audrey; McGovern, Stephen; Meile, Jean-Christophe; Errington, Jeffrey; Ehrlich, S Dusko; Noirot, Philippe; Polard, Patrice

    2007-10-01

    In bacteria, several salvage responses to DNA replication arrest culminate in reassembly of the replisome on inactivated forks to resume replication. The PriA DNA helicase is a prominent trigger of this replication restart process, preceded in many cases by a repair and/or remodeling of the arrested fork, which can be performed by many specific proteins. The mechanisms that target these rescue effectors to damaged forks in the cell are unknown. We report that the single-stranded DNA binding (SSB) protein is the key factor that links PriA to active chromosomal replication forks in vivo. This targeting mechanism determines the efficiency by which PriA reaches its specific DNA-binding site in vitro and directs replication restart in vivo. The RecG and RecQ DNA helicases, which are involved in intricate replication reactivation pathways, also associate with the chromosomal replication forks by similarly interacting with SSB. These results identify SSB as a platform for linking a 'repair toolbox' with active replication forks, providing a first line of rescue responses to accidental arrest.

  3. 77 FR 26733 - Uinta-Wasatch-Cache National Forest; Evanston-Mountain View Ranger District; Utah; Smiths Fork...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ... Fork Vegetation Restoration Project AGENCY: Forest Service, USDA. ACTION: Notice of intent to prepare... Forests Restoration Act (``HFRA''). DATES: Comments concerning the scope of the analysis must be received... Vegetation Restoration Project, Attn: Rick Schuler, P.O. Box 1880, Evanston, WY 82931. Comments can also...

  4. Gaps and forks in DNA replication: Rediscovering old models.

    PubMed

    Lehmann, Alan R; Fuchs, Robert P

    2006-12-01

    Most current models for replication past damaged lesions envisage that translesion synthesis occurs at the replication fork. However older models suggested that gaps were left opposite lesions to allow the replication fork to proceed, and these gaps were subsequently sealed behind the replication fork. Two recent articles lend support to the idea that bypass of the damage occurs behind the fork. In the first paper, electron micrographs of DNA replicated in UV-irradiated yeast cells show regions of single-stranded DNA both at the replication forks and behind the fork, the latter being consistent with the presence of gaps in the daughter-strands opposite lesions. The second paper describes an in vitro DNA replication system reconstituted from purified bacterial proteins. Repriming of synthesis downstream from a blocked fork occurred not only on the lagging strand as expected, but also on the leading strand, demonstrating that contrary to widely accepted beliefs, leading strand synthesis does not need to be continuous.

  5. Optimal control of information epidemics modeled as Maki Thompson rumors

    NASA Astrophysics Data System (ADS)

    Kandhway, Kundan; Kuri, Joy

    2014-12-01

    We model the spread of information in a homogeneously mixed population using the Maki Thompson rumor model. We formulate an optimal control problem, from the perspective of single campaigner, to maximize the spread of information when the campaign budget is fixed. Control signals, such as advertising in the mass media, attempt to convert ignorants and stiflers into spreaders. We show the existence of a solution to the optimal control problem when the campaigning incurs non-linear costs under the isoperimetric budget constraint. The solution employs Pontryagin's Minimum Principle and a modified version of forward backward sweep technique for numerical computation to accommodate the isoperimetric budget constraint. The techniques developed in this paper are general and can be applied to similar optimal control problems in other areas. We have allowed the spreading rate of the information epidemic to vary over the campaign duration to model practical situations when the interest level of the population in the subject of the campaign changes with time. The shape of the optimal control signal is studied for different model parameters and spreading rate profiles. We have also studied the variation of the optimal campaigning costs with respect to various model parameters. Results indicate that, for some model parameters, significant improvements can be achieved by the optimal strategy compared to the static control strategy. The static strategy respects the same budget constraint as the optimal strategy and has a constant value throughout the campaign horizon. This work finds application in election and social awareness campaigns, product advertising, movie promotion and crowdfunding campaigns.

  6. Community Learning Campus: It Takes a Simple Message to Build a Complex Project

    ERIC Educational Resources Information Center

    Pearson, George

    2012-01-01

    Education Canada asked Tom Thompson, president of Olds College and a prime mover behind the Community Learning Campus (CLC): What were the lessons learned from this unusually ambitious education project? Thompson mentions six lessons he learned from this complex project which include: (1) Dream big, build small, act now; (2) Keep a low profile at…

  7. Quartz tuning fork based microwave impedance microscopy

    NASA Astrophysics Data System (ADS)

    Cui, Yong-Tao; Ma, Eric Yue; Shen, Zhi-Xun

    2016-06-01

    Microwave impedance microscopy (MIM), a near-field microwave scanning probe technique, has become a powerful tool to characterize local electrical responses in solid state samples. We present the design of a new type of MIM sensor based on quartz tuning fork and electrochemically etched thin metal wires. Due to a higher aspect ratio tip and integration with tuning fork, such design achieves comparable MIM performance and enables easy self-sensing topography feedback in situations where the conventional optical feedback mechanism is not available, thus is complementary to microfabricated shielded stripline-type probes. The new design also enables stable differential mode MIM detection and multiple-frequency MIM measurements with a single sensor.

  8. The Australian Monstrilloida (Crustacea: Copepoda) II. Cymbasoma Thompson, 1888.

    PubMed

    Suárez-Morales, Eduardo; Mckinnon, A David

    2016-01-01

    Monstrilloid copepods collected during the past two decades from zooplankton surveys in reef and coastal areas of Australia were analyzed. A first contribution included the taxonomic analysis of three genera of the Monstrilloida, Monstrillopsis Sars, 1921, Maemonstrilla Grygier & Ohtsuka, 2008, and the newly described Australomonstrillopsis Suárez-Morales & McKinnon, 2014. In this document a taxonomic analysis of the species belonging to the genus Cymbasoma Thompson, 1888 is provided. A total of 28 species were found, most of them being undescribed. Seventeen species were described based on females only and eight on male specimens while three species were described from both sexes. Males of Australian species of Cymbasoma are distinguished by details of the genital complex, body size and proportions, ornamentation and processes of the cephalic region, number of caudal setae, and the characteristic structure or ornamentation of the genital lappets. Two main groups of males were distinguished on the basis of the number of caudal setae (3 or 4). As for the females, 20 of the 25 new species of Cymbasoma have fifth legs with an unarmed inner lobe and three setae on the outer lobe; one of these species (C. jinigudira sp. nov.) belongs to the C. longispinosum species-group (sensu Üstün et al. 2014). Another group, consisting of five species, has only two setae on the outer (exopodal) lobe. There were no Australian species of Cymbasoma with a single lobe. A species group, named after C. agoense, is proposed to include species sharing a globose body and a female fifth leg with a large endopodal lobe and an outer (exopodal) lobe with two setae. The females of the new species of Cymbasoma from Australia can be distinguished from their known congeners by unique combinations of characters including the type of body ornamentation, body size and shape, antennule armature and proportions, the presence of distinctive features of the legs 1-4, the presence/absence of processes on

  9. The E. coli DNA Replication Fork.

    PubMed

    Lewis, J S; Jergic, S; Dixon, N E

    2016-01-01

    DNA replication in Escherichia coli initiates at oriC, the origin of replication and proceeds bidirectionally, resulting in two replication forks that travel in opposite directions from the origin. Here, we focus on events at the replication fork. The replication machinery (or replisome), first assembled on both forks at oriC, contains the DnaB helicase for strand separation, and the DNA polymerase III holoenzyme (Pol III HE) for DNA synthesis. DnaB interacts transiently with the DnaG primase for RNA priming on both strands. The Pol III HE is made up of three subassemblies: (i) the αɛθ core polymerase complex that is present in two (or three) copies to simultaneously copy both DNA strands, (ii) the β2 sliding clamp that interacts with the core polymerase to ensure its processivity, and (iii) the seven-subunit clamp loader complex that loads β2 onto primer-template junctions and interacts with the α polymerase subunit of the core and the DnaB helicase to organize the two (or three) core polymerases. Here, we review the structures of the enzymatic components of replisomes, and the protein-protein and protein-DNA interactions that ensure they remain intact while undergoing substantial dynamic changes as they function to copy both the leading and lagging strands simultaneously during coordinated replication.

  10. Thompson calf squeezing test: clinical and ultrasound correlations in the follow up of Achille's tenorraphy.

    PubMed

    Leigheb, M; Conte, P; Neri, P; Zorzolo, I; Martinelli, D; Martino, F; Carriero, A; Grassi, F

    2014-09-24

    In the follow up of Achille's tenorraphy, negativization of Thompson calf queezing test is not always omogeneous and absolute. Aim of the paper is to correlate Thompson test to different anatomical-ultrasound and functional parameters. We investigated clinically and by ultrasound 61 patients operated on of Achille's tenorraphy at Novara Hospital with follow-up of 10 to 46 months. Negative controls were contralateral tendons. We excluded patients with previous and/or contralateral Achille's tendon ruptures, those operated after 7 days, diabetics or with autoimmune diseases, if used topic steroids, < 18 years, those rejecting the study. Measured parameters were: age, gender, height, weight, side, open vs percutaneous approach, time from operation, neutral angle and range of motion of the ankle, maximal circumference of the leg, Single Heel Rise Test, Visual-Analogue-Scale Foot and Ankle (VAS FA) score; with ultrasound: length of tendons, mio-tendinous U.S.-structure, dynamic diastasis of tendon scar, tendon sliding. Thompson test is positive if no plantar-flexion of the foot occurs at calf squeezing, negative if plantar-flexion is normal (75% patients) and intermediate if reduced or slight reactive (25%).We found correlation of Thompson test with age (p<0,05) and with tendon length (p>0,05), being intermediate tests more represented in older patients and in those with longer healed tendons. In conclusion post-operative negativization of Thompson test can be incomplete as observed in older patients and in those healed with elongated tendon.

  11. Checkpoint regulation of replication forks: global or local?

    PubMed

    Iyer, Divya Ramalingam; Rhind, Nicholas

    2013-12-01

    Cell-cycle checkpoints are generally global in nature: one unattached kinetochore prevents the segregation of all chromosomes; stalled replication forks inhibit late origin firing throughout the genome. A potential exception to this rule is the regulation of replication fork progression by the S-phase DNA damage checkpoint. In this case, it is possible that the checkpoint is global, and it slows all replication forks in the genome. However, it is also possible that the checkpoint acts locally at sites of DNA damage, and only slows those forks that encounter DNA damage. Whether the checkpoint regulates forks globally or locally has important mechanistic implications for how replication forks deal with damaged DNA during S-phase.

  12. A description of the hydrologic system and the effects of coal mining on water quality in the East Fork Little Chariton River and the alluvial aquifer between Macon and Huntsville, north-central Missouri

    USGS Publications Warehouse

    Hall, D.C.

    1986-01-01

    The quality of surface and groundwater has been affected by abandoned strip mines and by abandoned underground mines in a 110-sq mi subbasin of the East Fork Little Chariton River. More than 14% of the area was strip mined for coal before 1979. The hydrologic system in the area was investigated and the effects of coal mining on quality of water in the river and alluvial aquifer were analyzed, with major emphasis on defining strip-mining effects. The groundwater gradient was from glacial drift or coal spring to alluvium to the East Fork Little Chariton River, and was greatest in spring and least in fall. Seepage from alluvium to the East Fork Little Chariton River occurs throughout the year, except during drought conditions when the only river flow is water released from Long Branch Lake. In the East Fork Little Chariton River median dissolved-solids concentrations increased from 153 mg/L near Macon to 630 mg/L near Huntsville and median sulfate concentrations increased from 36 mg/L near Macon to 360 mg/L near Huntsville. The median dissolved-solids concentration in water from the alluvium increased from 408 mg/L upstream from the strip mines to 641 mg/L near the mines and median dissolved-sulfate concentration increased from 140 to 350 mg/L. The sulfate-to-chloride ratio, used as the most sensitive indicator of strip-mining effects, increased markedly downstream in the East Fork Little Chariton River and nearby Middle Fork Little Chariton River, which also is affected by strip mining. There were no significant increases in sulfate-to-chloride ratio and dissolved-solids concentrations in comparable nearby subbasins of the Grand, Thompson, and Chariton Rivers where there was no mining. (Author 's abstract)

  13. Evolution of tail fork depth in genus Hirundo.

    PubMed

    Hasegawa, Masaru; Arai, Emi; Kutsukake, Nobuyuki

    2016-02-01

    A classic example of a sexually selected trait, the deep fork tail of the barn swallow Hirundo rustica is now claimed to have evolved and be maintained mainly via aerodynamic advantage rather than sexually selected advantage. However, this aerodynamic advantage hypothesis does not clarify which flight habits select for/against deep fork tails, causing diversity of tail fork depth in hirundines. Here, by focusing on the genus Hirundo, we investigated whether the large variation in tail fork depth could be explained by the differential flight habits. Using a phylogenetic comparative approach, we found that migrant species had deeper fork tails, but less colorful plumage, than the other species, indicating that migration favors a specific trait, deep fork tails. At the same time, tail fork depth but not plumage coloration decreased with increasing bill size - a proxy of prey size, suggesting that foraging on larger prey items favors shallower fork tails. Variation of tail fork depth in the genus Hirundo may be explained by differential flight habits, even without assuming sexual selection. PMID:26865972

  14. BALD ROCK AND MIDDLE FORK FEATHER RIVER ROADLESS AREAS, CALIFORNIA.

    USGS Publications Warehouse

    Sorensen, Martin L.; Buehler, Alan R.

    1984-01-01

    The results of a mineral-resource assessment of the Bald Rock and Middle Fork Feather River Roadless Areas in California indicate several areas within the Middle Fork Feather River Roadless Area that have probable mineral-resource potential. A probable potential for placer gold exists at various localities, both in areas covered by Tertiary volcanic rocks and in small streams that drain into the Middle Fork of the Feather River. A probable potential for small deposits of chromite exists in tracts underlain by ultramafic rocks in the Melones fault zone. A probable potential for lead-silver deposits is recognized at the east end of the Middle Fork Feather River Roadless Area.

  15. Impediments to replication fork movement: stabilisation, reactivation and genome instability.

    PubMed

    Lambert, Sarah; Carr, Antony M

    2013-03-01

    Maintaining genome stability is essential for the accurate transmission of genetic material. Genetic instability is associated with human genome disorders and is a near-universal hallmark of cancer cells. Genetic variation is also the driving force of evolution, and a genome must therefore display adequate plasticity to evolve while remaining sufficiently stable to prevent mutations and chromosome rearrangements leading to a fitness disadvantage. A primary source of genome instability are errors that occur during chromosome replication. More specifically, obstacles to the movement of replication forks are known to underlie many of the gross chromosomal rearrangements seen both in human cells and in model organisms. Obstacles to replication fork progression destabilize the replisome (replication protein complex) and impact on the integrity of forked DNA structures. Therefore, to ensure the successful progression of a replication fork along with its associated replisome, several distinct strategies have evolved. First, there are well-orchestrated mechanisms that promote continued movement of forks through potential obstacles. Second, dedicated replisome and fork DNA stabilization pathways prevent the dysfunction of the replisome if its progress is halted. Third, should stabilisation fail, there are mechanisms to ensure damaged forks are accurately fused with a converging fork or, when necessary, re-associated with the replication proteins to continue replication. Here, we review what is known about potential barriers to replication fork progression, how these are tolerated and their impact on genome instability.

  16. M2-F1 on lakebed with pilots Milt Thompson, Chuck Yeager, Don Mallick, and Bruce Peterson

    NASA Technical Reports Server (NTRS)

    1963-01-01

    After the initial M2-F1 airtow flights, the NASA Flight Research Center used the vehicle to check out other pilots. Bruce Peterson was scheduled to take over as the M2-F1 project pilot from Milt Thompson, while Don Mallick was to be his backup. Col. (later Brig. Gen.) Charles (Chuck) Yeager, then commandant of the Air Force's Aerospace Research Pilots School, wanted to evaluate a possible lifting-body trainer for the school. This photo shows all of these distinguished pilots on or in the M2-F1, with Col. Yeager in the pilot's seat. The lifting body concept evolved in the mid-1950s as researchers considered alternatives to ballistic reentries of piloted space capsules. The designs for hypersonic, wingless vehicles were on the boards at NASA Ames and NASA Langley facilities, while the US Air Force was gearing up for its Dyna-Soar program, which defined the need for a spacecraft that would land like an airplane. Despite favorable research on lifting bodies, there was little support for a flight program. Dryden engineer R. Dale Reed was intrigued with the lifting body concept, and reasoned that some sort of flight demonstration was needed before wingless aircraft could be taken seriously. In February 1962, he built a model lifting body based upon the Ames M2 design, and air-launched it from a radio controlled 'mothership.' Home movies of these flights, plus the support of research pilot Milt Thompson, helped pursuade the facilities director, Paul Bikle, to give the go-ahead for the construction of a full-scale version, to be used as a wind-tunnel model and possibly flown as a glider. Comparing lifting bodies to space capsules, an unofficial motto of the project was, 'Don't be Rescued from Outer Space--Fly Back in Style.' The construction of the M2-F1 was a joint effort by Dryden and a local glider manufacturer, the Briegleb Glider Company. The budget was $30,000. NASA craftsmen and engineers built the tubular steel interior frame. Its mahogany plywood shell was hand

  17. Test pilots 1962 - Armstrong, Walker, Dana, Peterson, McKay, Thompson, Butchart

    NASA Technical Reports Server (NTRS)

    1962-01-01

    The research pilots at what in 1962 was called the Flight Research Center standing in front of the X-1E. They are (left to right) Neil Armstrong, Joe Walker, Bill Dana, Bruce Peterson, Jack McKay, Milt Thompson, and Stan Butchart. of the group, Armstrong, Walker, Dana, McKay and Thompson all flew the X-15. Bruce Peterson flew the M2-F2 and HL-10 lifting bodies, while Stan Butchart was the B-29 drop plane pilot for many of the D-558-II and X-1 series research aircraft.

  18. As-Built documentation of programs to implement the Robertson and Doraiswamy/Thompson models

    NASA Technical Reports Server (NTRS)

    Valenziano, D. J. (Principal Investigator)

    1981-01-01

    The software which implements two spring wheat phenology models is described. The main program routines for the Doraiswamy/Thompson crop phenology model and the basic Robertson crop phenology model are DTMAIN and BRMAIN. These routines read meteorological data files and coefficient files, accept the planting date information and other information from the user, and initiate processing. Daily processing for the basic Robertson program consists only of calculation of the basic Robertson increment of crop development. Additional processing in the Doraiswamy/Thompson program includes the calculation of a moisture stress index and correction of the basic increment of development. Output for both consists of listings of the daily results.

  19. Late Holocene environmental changes recorded in the sediments of Lago Thompson, Northern Chilean Patagonia

    NASA Astrophysics Data System (ADS)

    Fagel, Nathalie; Nuttin, Laurence; Bertrand, Sébastien; Borgniet, Geoffrey; Schmidt, Sabine; Araneda, Alberto; Torrejon, Fernando; Urrutia, Roberto

    2010-05-01

    We investigate the sedimentary record of Lago Thompson, a small lake (area 1.1 km2, watershed ~8 km2) located at an elevation of 750 masl in Northern Chilean Patagonia (45.38 °S, 71.47°W). At Present, the climate conditions around the lake are relatively dry, with annual precipitation averaging 500 mm. The goal of this study is to evaluate the natural climate variability over the Last Millennium in Northern Chilean Patagonia and its impact on the environment. The coring site was selected after a bathymetric survey using an echo-sounder. Several short cores were retrieved in 2008 using an Uwitec gravity corer: the length of the cores ranges between 124 and 132 cm. The preliminary age model is based on 4 AMS radiocarbon ages measured on bulk sediment and organic macro-remains. The radiocarbon ages demonstrate that the sediment cores cover the last 850 to 950 years. This sediment record, which is characterized by accumulation rates ranging between 1 and 3 mm/yr, can therefore be studied at decennial to centennial resolution. To reconstruct past changes in climate and the environment, we conducted a multiproxy study combining sedimentological, mineralogical and geochemical analyses (LOI 105, 550 and 950°C, biogenic silica content, magnetic susceptibility, tephrostratigraphy, bulk organic geochemistry, bulk mineralogy by X-ray diffraction, and inorganic geochemistry by ITRAX core scanner and ICP-AES). Visual descriptions and X-ray radiographies demonstrate that the sediment record is relatively undisturbed. The sediment is composed of light brown organic-rich clayey material. The lower part of the core (below 75 cm) is finely laminated whereas the upper part is more homogeneous. Magnetic susceptibility values highlight two tephra layers that represent explosive eruptions of volcanoes from the southern volcanic zone of Chile (Hudson, Cay, or Macá volcanoes). The biogenic silica content of the sediment retrieved in Lago Thompson is high (40 to 80%), and probably

  20. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED, GEORGIA

    EPA Science Inventory

    There is an urgent need for EPA to develop protocols for establishing Total Maximum Daily Loads (TMDLs) in streams, lakes and estuaries. A cooperative TMDL field data collection project between ORD and Region 4 is ongoing in the South Fork Broad River Watershed (SFBR), a 245.18 ...

  1. Surface-water quality assessment of the North Fork Red River basin upstream from Lake Altus, Oklahoma, 2002

    USGS Publications Warehouse

    Smith, S. Jerrod; Schneider, M.L.; Masoner, J.R.; Blazs, R.L.

    2003-01-01

    Elevated salinity in the North Fork Red River is a major concern of the Bureau of Reclamation W. C. Austin Project at Lake Altus. Understanding the relation between surface-water runoff, ground-water discharge, and surface-water quality is important for maintaining the beneficial use of water in the North Fork Red River basin. Agricultural practices, petroleum production, and natural dissolution of salt-bearing bedrock have the potential to influence the quality of nearby surface water. The U.S. Geological Survey, in cooperation with the Bureau of Reclamation, sampled stream discharge and water chemistry at 19 stations on the North Fork Red River and tributaries. To characterize surface-water resources of the basin in a systematic manner, samples were collected synoptically during receding streamflow conditions during July 8-11, 2002. Together, sulfate and chloride usually constitute greater than half of the dissolved solids. Concentrations of sulfate ranged from 87.1 to 3,450 milligrams per liter. The minimum value was measured at McClellan Creek near Back (07301220), and the maximum value was measured at Bronco Creek near Twitty (07301303). Concentrations of chloride ranged from 33.2 to 786 milligrams per liter. The minimum value was measured at a North Fork Red River tributary (unnamed) near Twitty (07301310), and the maximum value was measured at the North Fork Red River near Back (07301190), the most upstream sample station.

  2. 75 FR 8730 - Central Utah Project Completion Act; Intent To Prepare an Environmental Assessment for the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-25

    ... Realignment of a Portion of the Provo Reach of the Spanish Fork-Provo Reservoir Canal Pipeline AGENCY: Office... Spanish Fork-Provo Reservoir Canal Pipeline. This realignment is necessitated in order to avoid active and... associated with the Bonneville Unit of the Central Utah Project. The Spanish Fork-Provo Reservoir...

  3. 78 FR 16490 - Placer County Water Agency; Notice of Authorization for Continued Project Operation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... Operation On February 23, 2011, the Placer County Water Agency, licensee for the Middle Fork American River... Commission's regulations thereunder. The Middle Fork American River Hydroelectric Project is located on the Middle Fork American River and the Rubicon River and located in Placer and El Dorado Counties,...

  4. Complete genomic sequences of two outbreak strains of Salmonella enterica subsp. enterica serovar Thompson associated with cilantro

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Salmonella enterica subsp. enterica serovar Thompson strains RM1984 (CADPH-99A2334) and RM1986 (CADPH -99A2345) are clinical isolates from 1999, putatively related to an outbreak in California from contaminated cilantro. We report the complete genome sequences and annotation of these two S. Thompson...

  5. Complete Genome Sequences of Two Outbreak Strains of Salmonella enterica subsp. enterica Serovar Thompson Associated with Cilantro

    PubMed Central

    Huynh, Steven; Gorski, Lisa; Cooper, Kerry K.; Miller, William G.

    2015-01-01

    Salmonella enterica subsp. enterica serovar Thompson strains RM1984 (CADPH-99A2334) and RM1986 (CADPH-99A2345) are associated with a 1999 outbreak in contaminated cilantro. We report here the complete genome sequences and annotation of these two S. Thompson strains. These genomes are distinct and provide additional data for our understanding of S. enterica. PMID:26586897

  6. 33 CFR 110.48 - Thompson Cove on east side of Pawcatuck River below Westerly, R.I.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Thompson Cove on east side of Pawcatuck River below Westerly, R.I. 110.48 Section 110.48 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.48 Thompson Cove on east side of Pawcatuck River...

  7. 33 CFR 110.48 - Thompson Cove on east side of Pawcatuck River below Westerly, R.I.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Thompson Cove on east side of Pawcatuck River below Westerly, R.I. 110.48 Section 110.48 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.48 Thompson Cove on east side of Pawcatuck River...

  8. 33 CFR 110.48 - Thompson Cove on east side of Pawcatuck River below Westerly, R.I.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Thompson Cove on east side of Pawcatuck River below Westerly, R.I. 110.48 Section 110.48 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.48 Thompson Cove on east side of Pawcatuck River...

  9. 33 CFR 110.48 - Thompson Cove on east side of Pawcatuck River below Westerly, R.I.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Thompson Cove on east side of Pawcatuck River below Westerly, R.I. 110.48 Section 110.48 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.48 Thompson Cove on east side of Pawcatuck River...

  10. 33 CFR 110.48 - Thompson Cove on east side of Pawcatuck River below Westerly, R.I.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Thompson Cove on east side of Pawcatuck River below Westerly, R.I. 110.48 Section 110.48 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.48 Thompson Cove on east side of Pawcatuck River...

  11. Bedload measurements, East Fork River, Wyoming

    PubMed Central

    Leopold, Luna B.; Emmett, William W.

    1976-01-01

    A bedload trap in the riverbed provided direct quantitative measurement of debris-transport rate in the East Fork River, Wyoming, a basin of 466 km2 drainage area. Traction load moves only during the spring snow melt season. Data collected in three spring runoff seasons during which a peak flow of 45 m3/s occurred showed that transport rate is correlated with power expenditure of the flowing water and at high flows becomes directly proportional to power as suggested by Bagnold. PMID:16592302

  12. 33 CFR 117.307 - Miami River, North Fork.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Miami River, North Fork. 117.307 Section 117.307 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.307 Miami River, North Fork. The draw...

  13. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Big Fork River, Minn.; logging. 207.370 Section 207.370 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the...

  14. 33 CFR 117.307 - Miami River, North Fork.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Miami River, North Fork. 117.307 Section 117.307 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.307 Miami River, North Fork. The draw...

  15. 33 CFR 117.307 - Miami River, North Fork.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Miami River, North Fork. 117.307 Section 117.307 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.307 Miami River, North Fork. The draw...

  16. 33 CFR 117.315 - New River, South Fork.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false New River, South Fork. 117.315 Section 117.315 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.315 New River, South Fork. (a) The...

  17. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Big Fork River, Minn.; logging. 207.370 Section 207.370 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the...

  18. 33 CFR 117.307 - Miami River, North Fork.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Miami River, North Fork. 117.307 Section 117.307 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.307 Miami River, North Fork. The draw...

  19. 33 CFR 117.307 - Miami River, North Fork.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Miami River, North Fork. 117.307 Section 117.307 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.307 Miami River, North Fork. The draw...

  20. 33 CFR 117.315 - New River, South Fork.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false New River, South Fork. 117.315 Section 117.315 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.315 New River, South Fork. (a) The...

  1. Musical-grade tuning forks for emergent audiometric screening.

    PubMed

    McCormick, Rodger J; Poling, Mikaela I

    2015-06-11

    We discuss our experience using high-quality, musical-grade tuning forks for emergent audiometric screening in a 22-year-old woman with sudden-onset unilateral hearing sensation loss. We present a framework for using this method when proper audiometric equipment is unavailable but where musical-grade tuning forks can be rapidly supplied.

  2. 33 CFR 117.1063 - Willapa River South Fork.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Willapa River South Fork. 117.1063 Section 117.1063 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Fork. (a) The draw of the Washington State Parks and Recreation Commission bridge across the South...

  3. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Big Fork River, Minn.; logging. 207.370 Section 207.370 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the...

  4. 33 CFR 117.1063 - Willapa River South Fork.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Willapa River South Fork. 117.1063 Section 117.1063 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Fork. (a) The draw of the Washington State Parks and Recreation Commission bridge across the South...

  5. 33 CFR 117.315 - New River, South Fork.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false New River, South Fork. 117.315 Section 117.315 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.315 New River, South Fork. (a) The...

  6. 33 CFR 117.1063 - Willapa River South Fork.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Willapa River South Fork. 117.1063 Section 117.1063 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Fork. (a) The draw of the Washington State Parks and Recreation Commission bridge across the South...

  7. 33 CFR 117.315 - New River, South Fork.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false New River, South Fork. 117.315 Section 117.315 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.315 New River, South Fork. (a) The...

  8. 33 CFR 117.315 - New River, South Fork.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false New River, South Fork. 117.315 Section 117.315 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Florida § 117.315 New River, South Fork. (a) The...

  9. 33 CFR 207.370 - Big Fork River, Minn.; logging.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Big Fork River, Minn.; logging. 207.370 Section 207.370 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.370 Big Fork River, Minn.; logging. (a) During the...

  10. 33 CFR 117.1063 - Willapa River South Fork.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Willapa River South Fork. 117.1063 Section 117.1063 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Fork. (a) The draw of the Washington State Parks and Recreation Commission bridge across the South...

  11. 33 CFR 117.1063 - Willapa River South Fork.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Willapa River South Fork. 117.1063 Section 117.1063 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Fork. (a) The draw of the Washington State Parks and Recreation Commission bridge across the South...

  12. South Fork Telephone Switchboard Building, general view in setting showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, general view in setting showing (N) side; view (S) - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  13. South Fork Latrine, interior showing head with steel tank mounted ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine, interior showing head with steel tank mounted to wall; view south - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  14. South Fork Telephone Switchboard Building, interior west room showing hardwood ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, interior west room showing hardwood floor; view south - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  15. South Fork Telephone Switchboard Building, oblique view of (W) and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, oblique view of (W) and (S) sides, view to northeast - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  16. South Fork Telephone Switchboard Building, oblique view of east side; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Telephone Switchboard Building, oblique view of east side; view northwest - Fort McKinley, South Fork Telephone Switchboard Building, South side of Weymouth Way, approximately 100 feet west of East Side Drive, Great Diamond Island, Portland, Cumberland County, ME

  17. Preventing Replication Fork Collapse to Maintain Genome Integrity

    PubMed Central

    Cortez, David

    2015-01-01

    Billions of base pairs of DNA must be replicated trillions of times in a human lifetime. Complete and accurate replication once and only once per cell division cycle is essential to maintain genome integrity and prevent disease. Impediments to replication fork progression including difficult to replicate DNA sequences, conflicts with transcription, and DNA damage further add to the genome maintenance challenge. These obstacles frequently cause fork stalling, but only rarely cause a failure to complete replication. Robust mechanisms ensure that stalled forks remain stable and capable of either resuming DNA synthesis or being rescued by converging forks. However, when failures do happen the fork collapses leading to genome rearrangements, cell death and disease. Despite intense interest, the mechanisms to repair damaged replication forks, stabilize them, and ensure successful replication remain only partly understood. Different models of fork collapse have been proposed with varying descriptions of what happens to the DNA and replisome. Here, I will define fork collapse and describe what is known about how the replication checkpoint prevents it to maintain genome stability. PMID:25957489

  18. 14. Roaring Fork Motor Nature Trail, Place of a thousand ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Roaring Fork Motor Nature Trail, Place of a thousand drips, view with three culvert pipes. - Great Smoky Mountains National Park Roads & Bridges, Roaring Fork Motor Nature Trail, Between Cherokee Orchard Road & U.S. Route 321, Gatlinburg, Sevier County, TN

  19. Whose Confession? Which Tradition? (A Preliminary Critique of Penny Thompson, 2004)

    ERIC Educational Resources Information Center

    Doble, Peter

    2005-01-01

    What does Penny Thompson really want? Reading her article in "BJRE" 26 (1) proved a baffling experience: it clearly wanted to say something, and to say it passionately, yet signally failed to do so. It fails largely because it lacks an argument; there seems also to be conceptual muddle at its heart. A fuller critique will need to attend to…

  20. Religion, Idealism, and African American Autobiography in the Northern Plains: Era Bell Thompson's "American Daughter"

    ERIC Educational Resources Information Center

    Cole, Kevin; Weins, Leah

    2003-01-01

    Era Bell Thompson's "American Daughter", published in 1946 by the University of Chicago Press, is an autobiographical account of an African American woman who comes of age on the plains of North Dakota in the early twentieth century. It deserves to read and included in Great Plains studies because it recounts one of the rarest of American…

  1. CARBON TRACE GASES IN LAKE AND BEAVER POND ICE NEAR THOMPSON, MANITOBA, CANADA

    EPA Science Inventory

    Concentrations of CO2, CO, and CH4 were measured in beaver pond and lake ice in April 1996 near Thompson, Manitoba to derive information on possible impacts of ice melting on corresponding atmospheric trace gas concentrations. CH4 concentrations in beaver pond and lake ice ranged...

  2. An Evaluation Of Family Life Education At David Thompson Secondary School.

    ERIC Educational Resources Information Center

    Leslie, Susan

    The Family Life Education program at David Thompson Secondary School is a one semester course dealing with human sexuality. Given to eleventh grade students the course is directed at helping students understand the emotional and social dimensions of their sexuality. Because the emphasis in the course was on feeling, not fact, this evaluation of…

  3. Mary Ritter Beard and Marion Thompson Wright: Shaping Inclusive Social Education.

    ERIC Educational Resources Information Center

    Crocco, Margaret Smith

    1997-01-01

    Examines contributions by Mary Ritter Beard and Marion Thompson Wright to inclusive social education curricula. Beard established the field of women's history; Wright promoted the application of black history. Both saw social betterment as the goal of knowledge and sought inclusive understanding of the nature of legitimate knowledge in schools.…

  4. "This Strange White World": Race and Place in Era Bell Thompson's "American Daughter"

    ERIC Educational Resources Information Center

    Johnson, Michael K.

    2004-01-01

    Aboard a train heading out of Minneapolis toward frontier North Dakota, Era Bell Thompson in her autobiography "American Daughter" (1946) describes a landscape that grows steadily bleaker with each mile farther west: "Suddenly there was snow--miles and miles of dull, white snow, stretching out to meet the heavy, gray sky; deep banks of snow…

  5. 5. PILOTS KNIGHT, RUSHWORTH, ENGLE, THOMPSON, DANA, AND McKAY STANDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. PILOTS KNIGHT, RUSHWORTH, ENGLE, THOMPSON, DANA, AND McKAY STANDING AT THE NOSE OF X-15 NO. 66671. - Edwards Air Force Base, X-15 Engine Test Complex, Rogers Dry Lake, east of runway between North Base & South Base, Boron, Kern County, CA

  6. fork head domain genes in zebrafish.

    PubMed

    Odenthal, J; Nüsslein-Volhard, C

    1998-07-01

    Nine members of the fork head domain gene family (fkd1-fkd9) were isolated from early cDNA libraries in the zebrafish. They show unique expression patterns in whole-mount RNA in situ hybridization during the first 24 h of embryonic development. These fkd genes fall into three of ten classes, based on sequence similarities within the DNA-binding domain, whereas members for the other seven classes described in other vertebrates were not found. In addition to conserved residues at certain positions in the fork head domain, characteristic transcription activation domains as well as similarities in expression patterns were found for members of the different classes. Members of class I (fkd1/axial, fkd2/Zffkh1, fkd4 and fkd7) are differentially transcribed in unsegmented dorsal axial structures such as the floor plate, the notochord, the hypochord and, in addition, the endoderm. Transcripts of fkd3 and fkd5 (class II) are mainly detected in the cells of the ectoderm which form neural tissues, as is the case for genes of this class in other species. RNAs of the three members of class V (fkd6, fkd8 and fkd9) are expressed in the paraxial mesoderm and transiently in the neuroectoderm. fkd6 is strongly expressed in neural crest cells from early stages on, whereas fkd2 and fkd7 are transcribed in individual neural crest cells in the pharyngula period.

  7. Bull Trout Population and Habitat Surveys in the Middle Fork Willamette and McKenzie Rivers, Annual Report 2002.

    SciTech Connect

    Seals, Jason; Reis, Kelly

    2003-10-01

    Bull trout in the Willamette River Basin were historically distributed throughout major tributaries including the Middle Fork Willamette and McKenzie rivers. Habitat degradation, over-harvest, passage barriers, fish removal by rotenone, and hybridization and competition with non-native brook trout are all likely factors that have led to the decline of bull trout in the Willamette Basin (Ratliff and Howell 1992). The U.S. Fish and Wildlife Service listed the Columbia River bull trout population segment as Threatened under the federal Endangered Species Act in 1998. Four bull trout populations were isolated in the upper Willamette River following the construction of flood control dams on the South Fork McKenzie River, McKenzie River, and Middle Fork Willamette River that created Cougar, Trail Bridge, and Hills Creek reservoirs. Buchanan et al. (1997) described the population in the main stem McKenzie as 'of special concern', the South Fork McKenzie population as 'high risk of extinction', the population above Trail Bridge Reservoir as 'high risk of extinction', and bull trout in the Middle Fork Willamette as 'probably extinct'. Various management efforts such as strict angling regulations and passage improvement projects have been implemented to stabilize and rehabilitate bull trout habitat and populations in the McKenzie River over the past 10 years. Since 1997, bull trout fry from Anderson Creek on the upper McKenzie River have been transferred to the Middle Fork Willamette basin above Hills Creek Reservoir in an attempt to re-establish a reproducing bull trout population. This project was developed in response to concerns over the population status and management of bull trout in the McKenzie and Middle Fork Willamette Rivers by the Oregon Department of Fish and Wildlife during the early 1990s. The project was conducted under measure 9.3G(2) of the Columbia Basin Fish and Wildlife Program to monitor the status, life history, habitat needs, and limiting factors for

  8. PTEN regulates RPA1 and protects DNA replication forks.

    PubMed

    Wang, Guangxi; Li, Yang; Wang, Pan; Liang, Hui; Cui, Ming; Zhu, Minglu; Guo, Limei; Su, Qian; Sun, Yujie; McNutt, Michael A; Yin, Yuxin

    2015-11-01

    Tumor suppressor PTEN regulates cellular activities and controls genome stability through multiple mechanisms. In this study, we report that PTEN is necessary for the protection of DNA replication forks against replication stress. We show that deletion of PTEN leads to replication fork collapse and chromosomal instability upon fork stalling following nucleotide depletion induced by hydroxyurea. PTEN is physically associated with replication protein A 1 (RPA1) via the RPA1 C-terminal domain. STORM and iPOND reveal that PTEN is localized at replication sites and promotes RPA1 accumulation on replication forks. PTEN recruits the deubiquitinase OTUB1 to mediate RPA1 deubiquitination. RPA1 deletion confers a phenotype like that observed in PTEN knockout cells with stalling of replication forks. Expression of PTEN and RPA1 shows strong correlation in colorectal cancer. Heterozygous disruption of RPA1 promotes tumorigenesis in mice. These results demonstrate that PTEN is essential for DNA replication fork protection. We propose that RPA1 is a target of PTEN function in fork protection and that PTEN maintains genome stability through regulation of DNA replication.

  9. New interpretation of Clarks Fork field, northern Bighorn basin, Montana

    SciTech Connect

    Johnson, J.S.; Lindsley-Griffin, N.

    1986-08-01

    Clarks Fork field is located at the northern edge of the Bighorn basin (T9S, R22E) in Carbon County, Montana. Production was first established in 1944 by General Petroleum Corporation in the Cretaceous Peay Sandstone (basal Frontier) and was later extended to the Cretaceous Greybull (1949) and Lakota (1956) sandstones by British American. Total cumulative hydrocarbons from this field are 1,1789,193 bbl of oil and 3,061,522 mcf of gas, with Lakota sandstones being most productive. Lakota production occurs from a structural-stratigraphic trap in an east-west-trending channel on the axis of Clarks Fork anticline, geographically near the center of the township. Our structural reinterpretation of Clarks Fork field suggests that Elk Basin anticline is a northwest extension of the Elk Basin field anticline. The Elk Basin thrust truncates the north limb of the fold and does not strike to the northwest, as shown by earlier interpretations. They interpret a northwest-striking thrust in the center of the township as a splay off the Elk Basin thrust, and have named it the Clarks Fork thrust. The Clarks Fork anticline is located on the hanging wall of Clarks Fork thrust. Subsurface maps indicate the Clarks Fork area has not been fully developed. Stratigraphic traps in the Lakota and Greybull sandstones are present in several areas of the township. Structural traps in the center and northwest portions of the township may also exist.

  10. The Fork+ burnup measurement system: Design and first measurement campaign

    SciTech Connect

    Olson, C.E.; Bronowski, D.R.; McMurtry, W.; Ewing, R.; Jordan, R.; Rivard, D.

    1998-12-31

    Previous work with the original Fork detector showed that burnup as determined by reactor records could be accurately allocated to spent nuclear fuel assemblies. The original Fork detector, designed by Los Alamos National Laboratory, used an ion chamber to measure gross gamma count and a fission chamber to measure neutrons from an activation source, {sup 244}Cm. In its review of the draft Topical Report on Burnup Credit, the US Nuclear Regulatory Commission indicated it felt uncomfortable with a measurement system that depended on reactor records for calibration. The Fork+ system was developed at Sandia National Laboratories under the sponsorship of the Electric Power Research Institute with the aim of providing this independent measurement capability. The initial Fork+ prototype was used in a measurement campaign at the Maine Yankee reactor. The campaign confirmed the applicability of the sensor approach in the Fork+ system and the efficiency of the hand-portable Fork+ prototype in making fuel assembly measurements. It also indicated potential design modifications that will be necessary before the Fork+ can be used effectively on high-burnup spent fuel.

  11. Development of Tuning Fork Based Probes for Atomic Force Microscopy

    NASA Astrophysics Data System (ADS)

    Jalilian, Romaneh; Yazdanpanah, Mehdi M.; Torrez, Neil; Alizadeh, Amirali; Askari, Davood

    2014-03-01

    This article reports on the development of tuning fork-based AFM/STM probes in NaugaNeedles LLC for use in atomic force microscopy. These probes can be mounted on different carriers per customers' request. (e.g., RHK carrier, Omicron carrier, and tuning fork on a Sapphire disk). We are able to design and engineer tuning forks on any type of carrier used in the market. We can attach three types of tips on the edge of a tuning fork prong (i.e., growing Ag2Ga nanoneedles at any arbitrary angle, cantilever of AFM tip, and tungsten wire) with lengths from 100-500 μm. The nanoneedle is located vertical to the fork. Using a suitable insulation and metallic coating, we can make QPlus sensors that can detect tunneling current during the AFM scan. To make Qplus sensors, the entire quartz fork will be coated with an insulating material, before attaching the nanoneedle. Then, the top edge of one prong is coated with a thin layer of conductive metal and the nanoneedle is attached to the fork end of the metal coated prong. The metal coating provides electrical connection to the tip for tunneling current readout and to the electrodes and used to read the QPlus current. Since the amount of mass added to the fork is minimal, the resonance frequency spectrum does not change and still remains around 32.6 KHz and the Q factor is around 1,200 in ambient condition. These probes can enhance the performance of tuning fork based atomic microscopy.

  12. Wireless tuning fork gyroscope for biomedical applications

    NASA Astrophysics Data System (ADS)

    Abraham, Jose K.; Varadan, Vijay K.; Whitchurch, Ashwin K.; Sarukesi, K.

    2003-07-01

    This paper presents the development of a Bluetooth enabled wireless tuning fork gyroscope for the biomedical applications, including gait phase detection system, human motion analysis and physical therapy. This gyroscope is capable of measuring rotation rates between -90 and 90 and it can read the rotation information using a computer. Currently, the information from a gyroscope can trigger automobile airbag deployment during rollover, improve the accuracy and reliability of GPS navigation systems and stabilize moving platforms such as automobiles, airplanes, robots, antennas, and industrial equipment. Adding wireless capability to the existing gyroscope could help to expand its applications in many areas particularly in biomedical applications, where a continuous patient monitoring is quite difficult. This wireless system provides information on several aspects of activities of patients for real-time monitoring in hospitals.

  13. Fork gratings based on ferroelectric liquid crystals.

    PubMed

    Ma, Y; Wei, B Y; Shi, L Y; Srivastava, A K; Chigrinov, V G; Kwok, H-S; Hu, W; Lu, Y Q

    2016-03-21

    In this article, we disclose a fork grating (FG) based on the photo-aligned ferroelectric liquid crystal (FLC). The Digital Micro-mirror Device based system is used as a dynamic photomask to generated different holograms. Because of controlled anchoring energy, the photo alignment process offers optimal conditions for the multi-domain FLC alignment. Two different electro-optical modes namely DIFF/TRANS and DIFF/OFF switchable modes have been proposed where the diffraction can be switched either to no diffraction or to a completely black state, respectively. The FLC FG shows high diffraction efficiency and fast response time of 50µs that is relatively faster than existing technologies. Thus, the FLC FG may pave a good foundation toward optical vertices generation and manipulation that could find applications in a variety of devices. PMID:27136779

  14. Chemical and biological sensing using tuning forks

    SciTech Connect

    Tao, Nongjian; Boussaad, Salah

    2012-07-10

    A device for sensing a chemical analyte is disclosed. The device is comprised of a vibrating structure having first and second surfaces and having an associated resonant frequency and a wire coupled between the first and second surfaces of the vibrating structure, wherein the analyte interacts with the wire and causes a change in the resonant frequency of the vibrating structure. The vibrating structure can include a tuning fork. The vibrating structure can be comprised of quartz. The wire can be comprised of polymer. A plurality of vibrating structures are arranged in an array to increase confidence by promoting a redundancy of measurement or to detect a plurality of chemical analytes. A method of making a device for sensing a chemical analyte is also disclosed.

  15. Backwater at bridges and densely wooded flood plains, Thompson Creek near Clara, Mississippi

    USGS Publications Warehouse

    Colson, B.E.; Ming, C.O.; Arcement, George J.

    1979-01-01

    Floodflow data that will provide a base for evaluating digital models relating to open-channel flow were obtained at 22 sites on streams in Alabama, Louisiana, and Mississippi. Thirty-five floods were measured. Analysis of the data indicated methods currently in use would be inaccurate where densely vegetated flood plains are crossed by highway embankments and single-opening bridges. This atlas presents flood information at the site on Thompson Creek near Clara, MS: Water depths, velocities, and discharges through bridge openings on Thompson Creek near Clara, MS, for flood of March 3, 1971, are shown, together with peak water-surface elevations along embankments and along cross sections. Manning 's roughness coefficient values in different parts of the flood plain are shown on maps, and flood-frequency relations are shown on a graph. (USGS).

  16. 117. Laurel Fork Viaduct. Elevation view of this 545 1939 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    117. Laurel Fork Viaduct. Elevation view of this 545 1939 steel girder viaduct. Example of structure with plain reinforced concrete arches. Looking northwest. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  17. Assembly of Slx4 signaling complexes behind DNA replication forks.

    PubMed

    Balint, Attila; Kim, TaeHyung; Gallo, David; Cussiol, Jose Renato; Bastos de Oliveira, Francisco M; Yimit, Askar; Ou, Jiongwen; Nakato, Ryuichiro; Gurevich, Alexey; Shirahige, Katsuhiko; Smolka, Marcus B; Zhang, Zhaolei; Brown, Grant W

    2015-08-13

    Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress.

  18. 26. MOORSE DRILL CABINET AND FORK ART FABRICATED AT SHOP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. MOORSE DRILL CABINET AND FORK ART FABRICATED AT SHOP (L TO R)- LOOKING SOUTHEAST. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

  19. 8. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH OF LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING N. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  20. 9. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. EEL RIVER SOUTH FORK BRIDGE, OLD HIGHWAY 101. NORTH OF LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING W. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  1. Little Known Facts about the Common Tuning Fork.

    ERIC Educational Resources Information Center

    Ong, P. P.

    2002-01-01

    Explains the physical principles of the tuning fork which has a common use in teaching laboratories. Includes information on its vibration, frequency of vibration, elasticity, and reasons for having two prongs. (YDS)

  2. Assembly of Slx4 signaling complexes behind DNA replication forks

    PubMed Central

    Balint, Attila; Kim, TaeHyung; Gallo, David; Cussiol, Jose Renato; Bastos de Oliveira, Francisco M; Yimit, Askar; Ou, Jiongwen; Nakato, Ryuichiro; Gurevich, Alexey; Shirahige, Katsuhiko; Smolka, Marcus B; Zhang, Zhaolei; Brown, Grant W

    2015-01-01

    Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress. PMID:26113155

  3. 8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

  4. 9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW OF WHEEL RACK FOR BORING MILL. Fork loading crane, manufactured by Cleveland Tramrail, 2-1/2 ton capacity. - Juniata Shops, Erecting Shop & Machine Shop, East of Fourth Avenue, between Fourth & Fifth Streets, Altoona, Blair County, PA

  5. TRAIP regulates replication fork recovery and progression via PCNA

    PubMed Central

    Feng, Wanjuan; Guo, Yingying; Huang, Jun; Deng, Yiqun; Zang, Jianye; Huen, Michael Shing-Yan

    2016-01-01

    PCNA is a central scaffold that coordinately assembles replication and repair machineries at DNA replication forks for faithful genome duplication. Here, we describe TRAIP (RNF206) as a novel PCNA-interacting factor that has important roles during mammalian replicative stress responses. We show that TRAIP encodes a nucleolar protein that migrates to stalled replication forks, and that this is accomplished by its targeting of PCNA via an evolutionarily conserved PIP box on its C terminus. Accordingly, inactivation of TRAIP or its interaction with the PCNA clamp compromised replication fork recovery and progression, and leads to chromosome instability. Together, our findings establish TRAIP as a component of the mammalian replicative stress response network, and implicate the TRAIP-PCNA axis in recovery of stalled replication forks. PMID:27462463

  6. 17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. DETAIL VIEW OF WHAT APPEARS TO BE STIRRING FORK THAT MIXED COFFEE BEANS AS THEY WERE HUSKED - Hacienda Cafetalera Santa Clara, Coffee Mill, KM 19, PR Route 372, Hacienda La Juanita, Yauco Municipio, PR

  7. The MCM helicase: linking checkpoints to the replication fork.

    PubMed

    Forsburg, Susan L

    2008-02-01

    The MCM (minichromosome maintenance) complex is a helicase which is essential for DNA replication. Recent results suggest that the MCM helicase is important for replication fork integrity, and may function as a target of the replication checkpoint. Interactions between MCM proteins, checkpoint kinases, and repair and recovery proteins suggest that MCMs are proximal effectors of replication fork stability in the cell and are likely to play an important role in maintaining genome integrity. PMID:18208397

  8. Force regulated dynamics of RPA on a DNA fork

    PubMed Central

    Kemmerich, Felix E.; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

    2016-01-01

    Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg2+ concentrations, such that human RPA can melt DNA in absence of force. PMID:27016742

  9. Claspin promotes normal replication fork rates in human cells.

    PubMed

    Petermann, Eva; Helleday, Thomas; Caldecott, Keith W

    2008-06-01

    The S phase-specific adaptor protein Claspin mediates the checkpoint response to replication stress by facilitating phosphorylation of Chk1 by ataxia-telangiectasia and Rad3-related (ATR). Evidence suggests that these components of the ATR pathway also play a critical role during physiological S phase. Chk1 is required for high rates of global replication fork progression, and Claspin interacts with the replication machinery and might therefore monitor normal DNA replication. Here, we have used DNA fiber labeling to investigate, for the first time, whether human Claspin is required for high rates of replication fork progression during normal S phase. We report that Claspin-depleted HeLa and HCT116 cells display levels of replication fork slowing similar to those observed in Chk1-depleted cells. This was also true in primary human 1BR3 fibroblasts, albeit to a lesser extent, suggesting that Claspin is a universal requirement for high replication fork rates in human cells. Interestingly, Claspin-depleted cells retained significant levels of Chk1 phosphorylation at both Ser317 and Ser345, raising the possibility that Claspin function during normal fork progression may extend beyond facilitating phosphorylation of either individual residue. Consistent with this possibility, depletion of Chk1 and Claspin together doubled the percentage of very slow forks, compared with depletion of either protein alone.

  10. Force regulated dynamics of RPA on a DNA fork.

    PubMed

    Kemmerich, Felix E; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

    2016-07-01

    Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg(2+) concentrations, such that human RPA can melt DNA in absence of force.

  11. Gas Phase Photoacoustic Spectroscopy in the long-wave IR using Quartz Tuning Forks and Amplitude Modulated Quantum Cascade Lasers

    SciTech Connect

    Wojcik, Michael D.; Phillips, Mark C.; Cannon, Bret D.

    2006-12-31

    A paper to accompany a 20 minute talk about the progress of a DARPA funded project called LPAS. ABSTRACT: We demonstrate the performance of a novel long-wave infrared photoacoustic laser absorbance spectrometer for gas-phase species using an amplitude modulated (AM) quantum cascade (QC) laser and a quartz tuning fork microphone. Photoacoustic signal was generated by focusing the output of a Fabry-Perot QC laser operating at 8.41 micron between the legs of a quartz tuning fork which served as a transducer for the transient acoustic pressure wave. The QC laser was modulated at the resonant frequency of the tuning fork (32.8 kHz). This sensor was calibrated using the infrared absorber Freon-134a by performing a simultanious absorption measurement using a 35 cm absorption cell. The NEAS of this instrument was determined to be 2 x 10^-8 W cm^-1 /Hz^1/2 and the fundamental sensitivity of this technique is limited by the noise floor of the tuning fork itself.

  12. Acoustic Doppler current profiling from the JGOFS Arabian sea cruises aboard the RV T.G. Thompson

    SciTech Connect

    Kim, Hyun-Sook; Flagg, C.N.; Shi, Yan

    1996-06-01

    Acoustic Doppler current profiler (ADCP) data is part of the core data for the U.S. JGOFS Arabian Sea project, along with hydrographic and nutrient data. Seventeen cruises are scheduled to take place between September 1994 and January 1996 on the R/V T.G. Thompson. They are numbered consecutively from the ship`s commissioning with the first JGOFS cruise designated TN039. Table 1 lists start and end dates of each cruise with its mission. All but the first cruise have been or will be staged from Muscat, Oman. Each cruise is scheduled for a duration of between two weeks and one month. Seven of the cruises, referred to as process cruises, follow a standard cruise track, taking hydrographic, chemical and biological measurements. The rest of the cruises, which take place generally within the standard cruise region defined by a set track, are for the deployment and recovery of moored equipments and towing of a SeaSoar. ADCP data are collected using an autonomous data acquisition system developed for ship-of-opportunity cruises, named the AutoADCP system. The system is an extension of RD instrument`s DAS version 2.48 using enhancements made possible with {open_quotes}user-exit{close_quotes} programs. It makes it possible to collect ADCP data without the constant monitoring usually necessary and insures constant data coverage and uniform data quality.

  13. Best management practices plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    SciTech Connect

    1996-04-01

    This plan was prepared in support of the Phase II Remedial Design Report (DOE/OR/01-1449&D1) and in accordance with requirements under CERCLA to present the plan for best management practices to be followed during the remediation. This document provides the Environmental Restoration Program with information about spill prevention and control, water quality monitoring, good housekeeping practices, sediment and erosion control measures, and inspections and environmental compliance practices to be used during Phase II of the remediation project for the Lower East Fork Poplar Creek Operable Unit.

  14. Oxbow Conservation Area; Middle Fork John Day River, Annual Report 2001-2002.

    SciTech Connect

    Robertson, Shaun; Smith, Brent; Cochran, Brian

    2003-04-01

    In early 2001, the Confederated Tribes of Warm Springs, through their John Day Basin Office, concluded the acquisition of the Middle Fork Oxbow Ranch. Under a memorandum of agreement with the Bonneville Power Administration (BPA), the Tribes are required to provided BPA an 'annual written report generally describing the real property interests in the Project, HEP analyses undertaken or in progress, and management activities undertaken or in progress'. This report is to be provided to the BPA by 30 April of each year. This is the first annual report filed for the Oxbow Ranch property.

  15. Fascin, may the Forked be with you.

    PubMed

    Okenve-Ramos, Pilar; Llimargas, Marta

    2014-01-01

    The FGFR pathway triggers a wide range of key biological responses. Among others, the Breathless (Btl, Drosophila FGFR1) receptor cascade promotes cell migration during embryonic tracheal system development. However, how the actin cytoskeleton responds to Btl pathway activation to induce cell migration has remained largely unclear. Our recent results shed light into this issue by unveiling a link between the actin-bundling protein Singed (Sn) and the Btl pathway. We showed that the Btl pathway regulates sn, which leads to the stabilization of the actin bundles required for filopodia formation and actin cytoskeleton rearrangement. This regulation contributes to tracheal migration, tracheal branch fusion and tracheal cell elongation. Parallel actin bundles (PABs) are usually cross-linked by more than one actin-bundling protein. Accordingly, we have also shown that sn synergistically interacts with forked (f), another actin crosslinker. In this Extra View we extend f analysis and hypothesize how both actin-bundling proteins may act together to regulate the PABs during tracheal embryonic development. Although both proteins are required for similar tracheal events, we suggest that Sn is essential for actin bundle initiation and stiffening, while F is required for the lengthening and further stabilization of the PABs.

  16. A COMPREHENISVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    EPA Science Inventory

    There is an urgent need for EPA to develop protocols for establishing Total Maximum Daily Loads (TMDLs) in streams, lakes and estuaries. A cooperative TMDL field data collection project between ORD and Region 4 is ongoing in the South Fork Broad River Watershed (SFBR), a 245.18 ...

  17. Single strand transposition at the host replication fork

    PubMed Central

    Lavatine, Laure; He, Susu; Caumont-Sarcos, Anne; Guynet, Catherine; Marty, Brigitte; Chandler, Mick; Ton-Hoang, Bao

    2016-01-01

    Members of the IS200/IS605 insertion sequence family differ fundamentally from classical IS essentially by their specific single-strand (ss) transposition mechanism, orchestrated by the Y1 transposase, TnpA, a small HuH enzyme which recognizes and processes ss DNA substrates. Transposition occurs by the ‘peel and paste’ pathway composed of two steps: precise excision of the top strand as a circular ss DNA intermediate; and subsequent integration into a specific ssDNA target. Transposition of family members was experimentally shown or suggested by in silico high-throughput analysis to be intimately coupled to the lagging strand template of the replication fork. In this study, we investigated factors involved in replication fork targeting and analysed DNA-binding properties of the transposase which can assist localization of ss DNA substrates on the replication fork. We showed that TnpA interacts with the β sliding clamp, DnaN and recognizes DNA which mimics replication fork structures. We also showed that dsDNA can facilitate TnpA targeting ssDNA substrates. We analysed the effect of Ssb and RecA proteins on TnpA activity in vitro and showed that while RecA does not show a notable effect, Ssb inhibits integration. Finally we discuss the way(s) in which integration may be directed into ssDNA at the replication fork. PMID:27466393

  18. INTEGRATED OUTCROP AND SUBSURFACE STUDIES OF THE INTERWELL ENVIRONMENT OF CARBONATE RESERVOIRS: CLEAR FORK (LEONARDIAN AGE) RESERVOIRS, WEST TEXAS AND NEW MEXICO

    SciTech Connect

    F. Jerry Lucia; Stephen E. Laubach

    2000-04-01

    We have completed 18 months of this project and are reporting progress we have made in the areas of fracture and reservoir modeling. Task 1a: Construct Model of Outcrop Stratigraphic Framework has been completed. Task 1b: Construct Model of Fine-Scale Petrophysical Heterogeneity has been completed. Task 1c: Construct Model of Fracture Porosity and Permeability the object of this task is to characterize fracture porosity and distribution in the Wasson Clear Fork reservoir and to determine the effects of fractures on well performance. The approach is to measure fracture attributes in an analog outcrop, to develop models of fracture spacing and aperture, and to apply this information to the South Wasson Clear Fork reservoir. A data set has been collected from outcrops of the Clear Fork Formation, Apache Canyon, Sierra Diablo Mountains, West Texas, and a summary of the results is presented.

  19. Effects of ambient oxidant air pollution in the San Joaquin Valley on Thompson seedless grapes

    SciTech Connect

    Brewer, R.F.; Ashcroft, R.

    1984-01-01

    Mature Thompson seedless grape vines were enclosed in specially constructed plastic covered chambers supplied with carbon filtered and non-filtered (ambient) air from time of bud break through leaf drop. Effects on vegetative growth and fruiting were determined for three seasons. No effects on fruit production were measured the first season after covering but vegetative growth increased 12% in chambers supplied with filtered air. By the third season fruit yields were 27.5% higher in the filtered as compared with ambient chambers. The only visible symptoms associated with exposure to the oxidants was accelerated senescence which appeared 3 weeks to 1 month earlier on vines receiving ambient or nonfiltered air.

  20. The Sky Is Falling: A Study Of Particulates... . [Project ECOLogy ELE Pak, Thompson Pak].

    ERIC Educational Resources Information Center

    Thompson, Dennis W.

    This is one of a series of units for environmental education developed by the Highline Public Schools. Designed for secondary school science classes, the unit is concerned with particulate matter of air pollution. Five lessons are included. The lessons include construction of equipment and collecting data. (RH)

  1. A multi-fork z-axis quartz micromachined gyroscope.

    PubMed

    Feng, Lihui; Zhao, Ke; Sun, Yunan; Cui, Jianmin; Cui, Fang; Yang, Aiying

    2013-01-01

    A novel multi-fork z-axis gyroscope is presented in this paper. Different from traditional quartz gyroscopes, the lateral electrodes of the sense beam can be arranged in simple patterns; as a result, the fabrication is simplified. High sensitivity is achieved by the multi-fork design. The working principles are introduced, while the finite element method (FEM) is used to simulate the modal and sensitivity. A quartz fork is fabricated, and a prototype is assembled. Impedance testing shows that the drive frequency and sense frequency are similar to the simulations, and the quality factor is approximately 10,000 in air. The scale factor is measured to be 18.134 mV/(°/s) and the nonlinearity is 0.40% in a full-scale input range of ±250 °/s.

  2. A Multi-Fork Z-Axis Quartz Micromachined Gyroscope

    PubMed Central

    Feng, Lihui; Zhao, Ke; Sun, Yunan; Cui, Jianmin; Cui, Fang; Yang, Aiying

    2013-01-01

    A novel multi-fork z-axis gyroscope is presented in this paper. Different from traditional quartz gyroscopes, the lateral electrodes of the sense beam can be arranged in simple patterns; as a result, the fabrication is simplified. High sensitivity is achieved by the multi-fork design. The working principles are introduced, while the finite element method (FEM) is used to simulate the modal and sensitivity. A quartz fork is fabricated, and a prototype is assembled. Impedance testing shows that the drive frequency and sense frequency are similar to the simulations, and the quality factor is approximately 10,000 in air. The scale factor is measured to be 18.134 mV/(°/s) and the nonlinearity is 0.40% in a full-scale input range of ±250 °/s. PMID:24048339

  3. Characterization of the ATPase activity of RecG and RuvAB proteins on model fork structures reveals insight into stalled DNA replication fork repair.

    PubMed

    Abd Wahab, Syafiq; Choi, Meerim; Bianco, Piero R

    2013-09-13

    RecG and RuvAB are proposed to act at stalled DNA replication forks to facilitate replication restart. To clarify the roles of these proteins in fork regression, we used a coupled spectrophotometric ATPase assay to determine how these helicases act on two groups of model fork substrates: the first group mimics nascent stalled forks, whereas the second mimics regressed fork structures. The results show that RecG is active on the substrates in group 1, whereas these are poor substrates for RuvAB. In addition, in the presence of group 1 forks, the single-stranded DNA-binding protein (SSB) enhances the activity of RecG and enables it to compete with excess RuvA. In contrast, SSB inhibits the activity of RuvAB on these substrates. Results also show that the preferred regressed fork substrate for RuvAB is a Holliday junction, not a forked DNA. The active form of the enzyme on the Holliday junction contains a single RuvA tetramer. In contrast, although the enzyme is active on a regressed fork structure, RuvB loading by a single RuvA tetramer is impaired, and full activity requires the cooperative binding of two forked DNA substrate molecules. Collectively, the data support a model where RecG is responsible for stalled DNA replication fork regression. SSB ensures that if the nascent fork has single-stranded DNA character RuvAB is inhibited, whereas the activity of RecG is preferentially enhanced. Only once the fork has been regressed and the DNA is relaxed can RuvAB bind to a RecG-extruded Holliday junction.

  4. Bank stability and channel width adjustment, East Fork River, Wyoming.

    USGS Publications Warehouse

    Andrews, E.D.

    1982-01-01

    Frequent surveys of eight cross sections located in self-formed reaches of the East Fork River, Wyoming, during the 1974 snowmelt flood showed a close relation between channel morphology and scour and fill. Those cross sections narrower than the mean reach width filled at discharges less than bankfull and scoured at discharges greater than bankfull. Those cross sections wider than the mean reach width scoured at discharges less than bankfull and filled at discharges greater than bankfull. Bank stability, and to some extent the adjustment of stream channel width, in the East Fork River study reach appears to be controlled by the processes of scour and fill. -from Author

  5. 77 FR 71611 - Land Acquisitions; North Fork Rancheria of Mono Indians of California

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-03

    ... Bureau of Indian Affairs Land Acquisitions; North Fork Rancheria of Mono Indians of California AGENCY... trust for gaming purposes for the North Fork Rancheria of Mono Indians of California on November 26... land in trust for the North Fork Rancheria of Mono Indians of California under the authority of...

  6. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Requirements for fork and frame assembly... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  7. Recovery of arrested replication forks by homologous recombination is error-prone.

    PubMed

    Iraqui, Ismail; Chekkal, Yasmina; Jmari, Nada; Pietrobon, Violena; Fréon, Karine; Costes, Audrey; Lambert, Sarah A E

    2012-01-01

    Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability. Here, we report that recovery of impeded replication forks by homologous recombination is error-prone. Using a fork-arrest-based assay in fission yeast, we demonstrate that a single collapsed fork can cause mutations and large-scale genomic changes, including deletions and translocations. Fork-arrest-induced gross chromosomal rearrangements are mediated by inappropriate ectopic recombination events at the site of collapsed forks. Inverted repeats near the site of fork collapse stimulate large-scale genomic changes up to 1,500 times over spontaneous events. We also show that the high accuracy of DNA replication during S-phase is impaired by impediments to fork progression, since fork-arrest-induced mutation is due to erroneous DNA synthesis during recovery of replication forks. The mutations caused are small insertions/duplications between short tandem repeats (micro-homology) indicative of replication slippage. Our data establish that collapsed forks, but not stalled forks, recovered by homologous recombination are prone to replication slippage. The inaccuracy of DNA synthesis does not rely on PCNA ubiquitination or trans-lesion-synthesis DNA polymerases, and it is not counteracted by mismatch repair. We propose that deletions/insertions, mediated by micro-homology, leading to copy number variations during replication stress may arise by progression of error-prone replication forks restarted by homologous recombination.

  8. 33 CFR 208.33 - Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Fork of Ninnescah River, Kans. 208.33 Section 208.33 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork of Ninnescah River, Kans. The Bureau of Reclamation, or its designated agent, shall operate... flows in excess of bankfull on the North Fork of Ninnescah and Ninnescah River downstream of...

  9. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for fork and frame assembly... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  10. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Requirements for fork and frame assembly... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  11. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Requirements for fork and frame assembly... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  12. 33 CFR 208.33 - Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Fork of Ninnescah River, Kans. 208.33 Section 208.33 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork of Ninnescah River, Kans. The Bureau of Reclamation, or its designated agent, shall operate... flows in excess of bankfull on the North Fork of Ninnescah and Ninnescah River downstream of...

  13. 33 CFR 208.33 - Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Fork of Ninnescah River, Kans. 208.33 Section 208.33 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork of Ninnescah River, Kans. The Bureau of Reclamation, or its designated agent, shall operate... flows in excess of bankfull on the North Fork of Ninnescah and Ninnescah River downstream of...

  14. 33 CFR 208.33 - Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Fork of Ninnescah River, Kans. 208.33 Section 208.33 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork of Ninnescah River, Kans. The Bureau of Reclamation, or its designated agent, shall operate... flows in excess of bankfull on the North Fork of Ninnescah and Ninnescah River downstream of...

  15. 76 FR 62038 - Boundary Establishment for North Fork Crooked National Wild and Scenic River, Ochoco National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ... Forest Service Boundary Establishment for North Fork Crooked National Wild and Scenic River, Ochoco..., Washington Office, is transmitting the final boundary of the North Fork Crooked National Wild and Scenic.... SUPPLEMENTARY INFORMATION: The North Fork Crooked Wild and Scenic River boundary is available for review at...

  16. 16 CFR 1512.14 - Requirements for fork and frame assembly.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Requirements for fork and frame assembly... SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.14 Requirements for fork and frame assembly. The fork and frame assembly shall be tested for strength by application of a load of 890 N...

  17. 77 FR 39675 - Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ...; ] DEPARTMENT OF AGRICULTURE Forest Service Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt... changed to the Whitman District Ranger. This 2012 North Fork Burnt River Mining Record of Decision will replace and supercede the 2004 North Fork Burnt River Mining Record of Decision only where necessary...

  18. 33 CFR 208.33 - Cheney Dam and Reservoir, North Fork of Ninnescah River, Kans.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Fork of Ninnescah River, Kans. 208.33 Section 208.33 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork of Ninnescah River, Kans. The Bureau of Reclamation, or its designated agent, shall operate... flows in excess of bankfull on the North Fork of Ninnescah and Ninnescah River downstream of...

  19. Replication Termination: Containing Fork Fusion-Mediated Pathologies in Escherichia coli.

    PubMed

    Dimude, Juachi U; Midgley-Smith, Sarah L; Stein, Monja; Rudolph, Christian J

    2016-07-25

    Duplication of bacterial chromosomes is initiated via the assembly of two replication forks at a single defined origin. Forks proceed bi-directionally until they fuse in a specialised termination area opposite the origin. This area is flanked by polar replication fork pause sites that allow forks to enter but not to leave. The precise function of this replication fork trap has remained enigmatic, as no obvious phenotypes have been associated with its inactivation. However, the fork trap becomes a serious problem to cells if the second fork is stalled at an impediment, as replication cannot be completed, suggesting that a significant evolutionary advantage for maintaining this chromosomal arrangement must exist. Recently, we demonstrated that head-on fusion of replication forks can trigger over-replication of the chromosome. This over-replication is normally prevented by a number of proteins including RecG helicase and 3' exonucleases. However, even in the absence of these proteins it can be safely contained within the replication fork trap, highlighting that multiple systems might be involved in coordinating replication fork fusions. Here, we discuss whether considering the problems associated with head-on replication fork fusion events helps us to better understand the important role of the replication fork trap in cellular metabolism.

  20. Replication Termination: Containing Fork Fusion-Mediated Pathologies in Escherichia coli

    PubMed Central

    Dimude, Juachi U.; Midgley-Smith, Sarah L.; Stein, Monja; Rudolph, Christian J.

    2016-01-01

    Duplication of bacterial chromosomes is initiated via the assembly of two replication forks at a single defined origin. Forks proceed bi-directionally until they fuse in a specialised termination area opposite the origin. This area is flanked by polar replication fork pause sites that allow forks to enter but not to leave. The precise function of this replication fork trap has remained enigmatic, as no obvious phenotypes have been associated with its inactivation. However, the fork trap becomes a serious problem to cells if the second fork is stalled at an impediment, as replication cannot be completed, suggesting that a significant evolutionary advantage for maintaining this chromosomal arrangement must exist. Recently, we demonstrated that head-on fusion of replication forks can trigger over-replication of the chromosome. This over-replication is normally prevented by a number of proteins including RecG helicase and 3’ exonucleases. However, even in the absence of these proteins it can be safely contained within the replication fork trap, highlighting that multiple systems might be involved in coordinating replication fork fusions. Here, we discuss whether considering the problems associated with head-on replication fork fusion events helps us to better understand the important role of the replication fork trap in cellular metabolism. PMID:27463728

  1. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... contract for Big South Fork National Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public... National Park Service Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY... contract for the conduct of certain visitor services within Big South Fork National Recreation...

  2. 76 FR 6114 - Lincoln National Forest, New Mexico, North Fork Eagle Creek Wells Special Use Authorization

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ... North Fork well field (Village of Ruidoso 2006). The Village of Ruidoso drilled four production wells on...; ] DEPARTMENT OF AGRICULTURE Forest Service Lincoln National Forest, New Mexico, North Fork Eagle Creek Wells... wells on the North Fork of Eagle Creek, located on National Forest System land. The new permit...

  3. Evaluation of the Dallas Thompson Riverscreen Site on the Touchet River.

    SciTech Connect

    Chamness, Mickie

    2007-07-25

    Riverscreen irrigation pumps are a relatively new design in which the pump intake floats on the river surface, pulling water in only from the bottom side and surrounded by a self-cleaning screen. The Walla Walla County Conservation District recently started replacing old pump screens with the Riverscreen and was interested in whether the screens are protective of juvenile salmonids. Pacific Northwest National Laboratory evaluated approach velocities and operations at the Riverscreen installation on the Dallas Thompson property, approximately 3 mi. north of Touchet, Washington and 300 ft north of Hofer Dam, on June 18, 2007. Evaluation of this site consisted of underwater videography and water velocity measurements. The Dallas Thompson Riverscreen was pumping approximately 930 gpm during our evaluation, which is close to the maximum pumping rate for this model. Underwater videography showed only slow movement of water-borne particulates toward the pump intake, and the screen material was clean. All water velocity measurements were taken below the pump intake opening and between 3 to 6 in. from the screen face. All approach velocities (flow toward the screen and pump) were below National Marine Fisheries Service draft guidelines for juvenile fish screens.

  4. Test pilots 1962 - Thompson, McKay, Dana, Armstrong, Peterson, Butchart, Walker

    NASA Technical Reports Server (NTRS)

    1962-01-01

    A group photo of NASA research pilots at the front door of the Flight Research Center headquarters building. In the front row are (left to right) Milt Thompson, Jack McKay, and Bill Dana. All three flew the X-15, and Thompson and Dana were also involved in the lifting body flights. McKay was injured in a crash landing in X-15 #2. Although he recovered, the injuries eventually forced him to retire from research flying. In the back row (left to right) are Neil Armstrong, Bruce Peterson, Stanley Butchart, and Joe Walker. Armstrong and Walker also both flew the X-15. Soon after this photo was taken, Armstrong was selected as an astronaut, and seven years later became the first man to walk on the Moon. Walker made the highest flight in the X-15, reaching 354,200 feet. He then went on to fly the Lunar Landing Research Vehicle, and was killed on June 8, 1966 when his F-104N collided with the XB-70. Peterson made the first flight in the HL-10 lifting body, and was later badly injured in the crash of the M2-F2 lifting body. Butchart flew a wide range of research missions in the 1950s, and was the B-29 drop plane pilot for a number of rocket flight.

  5. Evaluation of essential oils for maintaining postharvest quality of Thompson seedless table grape.

    PubMed

    Abdollahi, Ali; Hassani, Abbas; Ghosta, Youbert; Bernousi, Iraj; Meshkatalsadat, Mohammad Hadi; Shabani, Razieh; Ziaee, Seyed Masoud

    2012-01-01

    The effects of postharvest spraying of essential oils from sweet basil (Ocimum basilicum), fennel (Foeniculum vulgare), summer savory (Satureja hortensis) and thyme (Thymus vulgaris) on fungal decay and quality parameters of the 'Thompson seedless' table grape stored at 0 ± 1°C for 60 days were evaluated. Results showed that the essential oils, especially of thyme and fennel, have a good inhibitory effect on the development of fungal decay in Thompson table grapes. In addition, essential oils reduced weight loss, berry and rachis browning and had no considerable adverse effect on the flavour of the fruits. GC-MS analysis showed that the main compounds identified in sweet basil, fennel, summer savory and thyme oils are linalool (65.25%), trans-anethole (64.72%), carvacrol (54.14%) and β-ocimene (12.62%), respectively. Therefore, these essential oils have good potential for use as an alternative to synthetic fungicides for the preservation and storage of table grapes. PMID:21999332

  6. Large outbreak of Salmonella Thompson related to smoked salmon in the Netherlands, August to December 2012.

    PubMed

    Friesema, I; de Jong, A; Hofhuis, A; Heck, M; van den Kerkhof, H; de Jonge, R; Hameryck, D; Nagel, K; van Vilsteren, G; van Beek, P; Notermans, D; van Pelt, W

    2014-10-02

    On 15 August 2012, an increase in the number of Salmonella Thompson cases was noticed by the Salmonella surveillance in the Netherlands. A case–control study was performed, followed by a food investigation. In total 1,149 cases were laboratory-confirmed between August and December 2012 of which four elderly (76–91 years) were reported to have died due to the infection. The cause of the outbreak was smoked salmon processed at a single site. The smoked salmon had been continuously contaminated in the processing lines through reusable dishes, which turned out to be porous and had become loaded with bacteria. This is the largest outbreak of salmonellosis ever recorded in the Netherlands. The temporary closure of the processing site and recall of the smoked salmon stopped the outbreak. An estimated four to six million Dutch residents were possibly exposed to the contaminated smoked salmon and an estimated 23,000 persons would have had acute gastroenteritis with S. Thompson during this outbreak. This outbreak showed that close collaboration between diagnostic laboratories, regional public health services, the national institute for public health and the food safety authorities is essential in outbreak investigations.

  7. South Fork Salmon River Watershed Restoration, 2008-2009 Annual Report.

    SciTech Connect

    Reaney, Mark D.

    2009-04-15

    The watershed restoration work elements within the project area, the South Fork Salmon River Watershed, follow the watershed restoration approach adopted by the Nez Perce Tribe Department of Fisheries Resource Management (DFRM) - Watershed Division. The vision of the Nez Perce Tribe DFRM-Watershed Division focuses on protecting, restoring, and enhancing watersheds and treaty resources within the ceded territory of the Nez Perce Tribe under the Treaty of 1855 with the United States Federal Government. The program uses a holistic approach, which encompasses entire watersheds, ridge top to ridge top, emphasizing all cultural aspects and strategies that rely on natural fish production and healthy river ecosystems. The Nez Perce Tribe DFRM-Watershed Division strives towards maximizing historic ecosystem productivity and health for the restoration of anadromous and resident fish populations and the habitat on which all depend on for future generations Originally, this project was funded to create a step/pool stream channel that was appropriate to restore fish passage where the 'Glory Hole Cascade' is currently located at the Stibnite Mine. Due to unforeseen circumstances at the time, the project is unable to move forward as planned and a request for a change in scope of the project and an expansion of the geographic area in which to complete project work was submitted. No additional funds were being requested. The ultimate goal of this project is to work with the holistic, ridge top to ridge top approach to protect and restore the ecological and biological functions of the South Fork Salmon River Watershed to assist in the recovery of threatened and endangered anadromous and resident fish species. FY 2008 Work Elements included two aquatic organism passage (AOP) projects to restore habitat connectivity to two fish-bearing tributaries to the East Fork South Fork Salmon River, Salt and Profile Creeks. The Work Elements also included road survey and assessment activities

  8. Effect of heat treatment, pH, sugar concentration, and metal addition on green color retention in homogenized puree of Thompson seedless grape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Homogenized puree of Thompson seedless (Vitis vinifera ‘Thompson Seedless’) grape was treated under different conditions, including heating time (5-30 min), temperature (20-80°C) and pH (2-10). Treatments with separate additions of glucose, fructose, and sucrose at concentrations of 100-600 g/L and ...

  9. Ten Things You Should Do with a Tuning Fork

    ERIC Educational Resources Information Center

    Lincoln, James

    2013-01-01

    Tuning forks are wonderful tools for teaching physics. Every physics classroom should have several and every physics student should be taught how to use them. In this article, I highlight 10 enriching demonstrations that most teachers might not know, as well as provide tips to enhance the demonstrations teachers might already be doing. Some of…

  10. 80. Laurel Fork Creek Bridge #2. Example of a concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    80. Laurel Fork Creek Bridge #2. Example of a concrete slab bridge with T beams. It was built in 1937 and the wing walls were faced with stone to blend with its surroundings. Looking northeast. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  11. New histone supply regulates replication fork speed and PCNA unloading

    PubMed Central

    Mejlvang, Jakob; Feng, Yunpeng; Alabert, Constance; Neelsen, Kai J.; Jasencakova, Zuzana; Zhao, Xiaobei; Lees, Michael; Sandelin, Albin; Pasero, Philippe; Lopes, Massimo

    2014-01-01

    Correct duplication of DNA sequence and its organization into chromatin is central to genome function and stability. However, it remains unclear how cells coordinate DNA synthesis with provision of new histones for chromatin assembly to ensure chromosomal stability. In this paper, we show that replication fork speed is dependent on new histone supply and efficient nucleosome assembly. Inhibition of canonical histone biosynthesis impaired replication fork progression and reduced nucleosome occupancy on newly synthesized DNA. Replication forks initially remained stable without activation of conventional checkpoints, although prolonged histone deficiency generated DNA damage. PCNA accumulated on newly synthesized DNA in cells lacking new histones, possibly to maintain opportunity for CAF-1 recruitment and nucleosome assembly. Consistent with this, in vitro and in vivo analysis showed that PCNA unloading is delayed in the absence of nucleosome assembly. We propose that coupling of fork speed and PCNA unloading to nucleosome assembly provides a simple mechanism to adjust DNA replication and maintain chromatin integrity during transient histone shortage. PMID:24379417

  12. Mechanisms of dissipation of an oscillating quartz tuning fork immersed in He II at high pressures

    NASA Astrophysics Data System (ADS)

    Gritsenko, I. A.; Zadorozhko, A. A.; Sheshin, G. A.

    2012-12-01

    The dissipative processes that occur with immersing a vibrating tuning fork in superfluid helium are investigated. The tuning forks resonance width Δf of frequencies from 32 to 97 kHz was measured in the temperature range from 0.2 to 2.5 K and He II pressure from SVP to 24.9 atm. Some of the tuning forks were in the original can (closed tuning fork), and for some tuning forks the can was either completely or partially removed (opened fork). We found that for the open tuning forks two dissipation mechanisms are clearly revealed in the temperature dependence of Δf, namely, acoustic radiation and scattering of ballistic thermal excitations at low temperatures, and viscous friction at high temperatures. At low temperatures (below ˜ 0.8 K) acoustic dissipation dominates, and the model of quadrupole oscillator for a tuning fork can be applied. We found that acoustic radiation for closed tuning forks is less effective and appears at lower temperatures. The first experimental data on dissipative processes in the quartz tuning fork-He II system at increased liquid pressures are obtained. It is shown that, for high frequency tuning forks the resonance bandwidth decreases with increasing pressure, i.e., with increasing wavelength of sound λ, according to the law λ-5. At low frequencies and low temperatures, with increasing mean free path of thermal excitations the resonance bandwidth is well described by the model of ballistic scattering.

  13. BOD1L Is Required to Suppress Deleterious Resection of Stressed Replication Forks.

    PubMed

    Higgs, Martin R; Reynolds, John J; Winczura, Alicja; Blackford, Andrew N; Borel, Valérie; Miller, Edward S; Zlatanou, Anastasia; Nieminuszczy, Jadwiga; Ryan, Ellis L; Davies, Nicholas J; Stankovic, Tatjana; Boulton, Simon J; Niedzwiedz, Wojciech; Stewart, Grant S

    2015-08-01

    Recognition and repair of damaged replication forks are essential to maintain genome stability and are coordinated by the combined action of the Fanconi anemia and homologous recombination pathways. These pathways are vital to protect stalled replication forks from uncontrolled nucleolytic activity, which otherwise causes irreparable genomic damage. Here, we identify BOD1L as a component of this fork protection pathway, which safeguards genome stability after replication stress. Loss of BOD1L confers exquisite cellular sensitivity to replication stress and uncontrolled resection of damaged replication forks, due to a failure to stabilize RAD51 at these forks. Blocking DNA2-dependent resection, or downregulation of the helicases BLM and FBH1, suppresses both catastrophic fork processing and the accumulation of chromosomal damage in BOD1L-deficient cells. Thus, our work implicates BOD1L as a critical regulator of genome integrity that restrains nucleolytic degradation of damaged replication forks.

  14. Termination of DNA replication forks: "Breaking up is hard to do".

    PubMed

    Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

    2015-01-01

    To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

  15. RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks.

    PubMed

    Elia, Andrew E H; Wang, David C; Willis, Nicholas A; Boardman, Alexander P; Hajdu, Ildiko; Adeyemi, Richard O; Lowry, Elizabeth; Gygi, Steven P; Scully, Ralph; Elledge, Stephen J

    2015-10-15

    We have used quantitative proteomics to profile ubiquitination in the DNA damage response (DDR). We demonstrate that RPA, which functions as a protein scaffold in the replication stress response, is multiply ubiquitinated upon replication fork stalling. Ubiquitination of RPA occurs on chromatin, involves sites outside its DNA binding channel, does not cause proteasomal degradation, and increases under conditions of fork collapse, suggesting a role in repair at stalled forks. We demonstrate that the E3 ligase RFWD3 mediates RPA ubiquitination. RFWD3 is necessary for replication fork restart, normal repair kinetics during replication stress, and homologous recombination (HR) at stalled replication forks. Mutational analysis suggests that multisite ubiquitination of the entire RPA complex is responsible for repair at stalled forks. Multisite protein group sumoylation is known to promote HR in yeast. Our findings reveal a similar requirement for multisite protein group ubiquitination during HR at stalled forks in mammalian cells.

  16. Termination of DNA replication forks: “Breaking up is hard to do”

    PubMed Central

    Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

    2015-01-01

    To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component – Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field. PMID:25835602

  17. Mitomycin C reduces abundance of replication forks but not rates of fork progression in primary and transformed human cells

    PubMed Central

    Kehrli, Keffy; Sidorova, Julia M.

    2014-01-01

    DNA crosslinks can block replication in vitro and slow down S phase progression in vivo. We characterized the effect of mitomycin C crosslinker on S phase globally and on individual replication forks in wild type and FANCD2-deficient human cells. FANCD2 is critical to crosslink repair, and is also implicated in facilitating DNA replication. We used DNA fiber analysis to demonstrate persistent reduction in abundance but not progression rate of replication forks during an S phase of MMC-treated cells. FANCD2 deficiency did not eliminate this phenotype. Immunoprecipitation of EdU-labeled DNA indicated that replication was not suppressed in the domains that were undergoing response to MMC as marked by the presence of γH2AX, and in fact γH2AX was overrepresented on DNA that had replicated immediately after MMC in wild type through less so in FANCD2-depleted cells. FANCD2-depleted cells also produced fewer tracks of uninterrupted replication of up to 240Kb long, regardless of MMC treatment. Overall, the data suggest that crosslinks may not pose a block to S phase as a whole, but instead profoundly change its progress by reducing density of replication forks and causing at least a fraction of forks to operate within a DNA damage response-altered chromatin. PMID:25580447

  18. Watershed characterization for precipitation-runoff modeling system, north fork, American River and east fork, Carson River watersheds, California

    USGS Publications Warehouse

    Smith, J. LaRue; Reece, Brian D.

    1995-01-01

    As part of its Global Change Hydrology Program, the U.S. Geological Survey (USGS) is investigating the potential effects of climate change on the water resources of several river basins in the United States. The American River Basin in California represents the windward slope of the north-central Sierra Nevada, and the California part of the Carson River Basin, most of which is in Nevada, represents the leeward slope. Parts of the American River and Carson River Basins—the North Fork American River and East Fork Carson River watersheds, both in California—were studied to determine the sensitivity of water resources to potential climate change. The water resources of both basins are derived primarily from snowmelt. A geographic information system (GIS) data base has been created to facilitate paired-basin analysis. The GIS data base incorporates (1) land-surface data, which include elevation, land use and land cover, soil type, and geology; (2) hydrologic data, such as stream networks and streamflow-gaging stations; and (3) climatic data, such as snow-course, snow-telemetry, radiosonde, and meteorological data. Precipitation-runoff models were developed and calibrated for the North Fork watershed within the American River Basin and for the East Fork watershed within the Carson River Basin. (These watersheds were selected to represent the climatic and physiographic variability of the two larger basins.) Synthesized climate scenarios then were used in the model to predict potential effects of climate change.

  19. Thompson and Hamilton type IV Freiberg's disease with involvement of multiple epiphyses of both feet.

    PubMed

    Lui, Tun Hing

    2015-01-01

    A 17-year-old boy reported left second and third toe pain after axial loading injury to his left foot. Radiographs showed collapse of the second metatarsal heads and epiphysial irregularities of the fifth metatarsal heads and the condyle of the proximal phalanx of the hallux of both feet. The patient was diagnosed to have Thompson and Hamilton type IV Freiberg's disease. He was screened for epiphysial dysplasia of the other sites. He had on and off bilateral hip and knee pain. Radiographs showed bilateral symmetrical epiphysial abnormalities with morphological change as focal concavity in bilateral femoral heads and fragmentation of the patellar articular surface with preservation of the patellofemoral joint space. PMID:25721826

  20. Moore-Gibson-Thompson equation with memory, part I: exponential decay of energy

    NASA Astrophysics Data System (ADS)

    Lasiecka, Irena; Wang, Xiaojun

    2016-04-01

    We are interested in the Moore-Gibson-Thompson equation with memory τ{u}_{ttt}+ α u_{tt}+c2Au+bAu_t -int_0tg(t-s)A w(s){d}s=0. This model arises in high-frequency ultrasound applications accounting for thermal flux and molecular relaxation times. According to revisited extended irreversible thermodynamics, thermal flux relaxation leads to the third-order derivative in time while molecular relaxation leads to non-local effects governed by memory terms. The resulting model is of hyperbolic type with viscous effects. We first classify the memory into three types. Then, we study how a memory term creates damping mechanism and how the memory causes energy decay even in the cases when the original memoryless system is unstable.

  1. Optimal information provision for maximizing flow in a forked lattice

    NASA Astrophysics Data System (ADS)

    Imai, Takeaki; Nishinari, Katsuhiro

    2015-06-01

    In a forked road, the provision of inappropriate information to car drivers sometimes leads to undesirable situations such as one-sided congestion, which is called the hunting phenomenon in real traffic. To address such problems, we propose a forked exclusion model and investigate the behavior of traffic flow in two routes, providing various types of information to a limited number of traveling particles according to the share rate of information. To analytically understand the phenomena, we develop a coarse-grained representation of the model. By analyzing the model, we find the most effective types of information to minimize particles' travel time and the existence of an optimal share rate according to route conditions.

  2. Optimal information provision for maximizing flow in a forked lattice.

    PubMed

    Imai, Takeaki; Nishinari, Katsuhiro

    2015-06-01

    In a forked road, the provision of inappropriate information to car drivers sometimes leads to undesirable situations such as one-sided congestion, which is called the hunting phenomenon in real traffic. To address such problems, we propose a forked exclusion model and investigate the behavior of traffic flow in two routes, providing various types of information to a limited number of traveling particles according to the share rate of information. To analytically understand the phenomena, we develop a coarse-grained representation of the model. By analyzing the model, we find the most effective types of information to minimize particles' travel time and the existence of an optimal share rate according to route conditions.

  3. A molecular tuning fork in single-molecule mechanochemical sensing.

    PubMed

    Mandal, Shankar; Koirala, Deepak; Selvam, Sangeetha; Ghimire, Chiran; Mao, Hanbin

    2015-06-22

    The separate arrangement of target recognition and signal transduction in conventional biosensors often compromises the real-time response and can introduce additional noise. To address these issues, we combined analyte recognition and signal reporting by mechanochemical coupling in a single-molecule DNA template. We incorporated a DNA hairpin as a mechanophore in the template, which, under a specific force, undergoes stochastic transitions between folded and unfolded hairpin structures (mechanoescence). Reminiscent of a tuning fork that vibrates at a fixed frequency, the device was classified as a molecular tuning fork (MTF). By monitoring the lifetime of the folded and unfolded hairpins with equal populations, we were able to differentiate between the mono- and bivalent binding modes during individual antibody-antigen binding events. We anticipate these mechanospectroscopic concepts and methods will be instrumental for the development of novel bioanalyses.

  4. The verification of reactor operating history using the fork detector

    SciTech Connect

    Menlove, H.O.; Reilly, T.D.; Siebelist, R.

    1996-07-01

    A technique has been developed for verification of light-water reactor operating history from measurements of irradiated fuel assemblies. The Fork detector is used to measure neutron and gross gamma-ray emissions from fuel assemblies. The measurements can be performed a few days after discharge or up to several years later. The neutron and gamma-ray ratios are used to check the consistency of the declared number of irradiated cycles for the assembly in the core. Reactor burnup calculation codes are used to correct the measured neutron rates for different initial enrichments and discontinuous irradiation histories. We have modified the Fork detector so that it can operate in the intense gamma-ray field emitted from freshly discharged fuel. This modification makes it possible to perform fuel verification during the annual fuel-reload and maintenance period.

  5. Optimal information provision for maximizing flow in a forked lattice.

    PubMed

    Imai, Takeaki; Nishinari, Katsuhiro

    2015-06-01

    In a forked road, the provision of inappropriate information to car drivers sometimes leads to undesirable situations such as one-sided congestion, which is called the hunting phenomenon in real traffic. To address such problems, we propose a forked exclusion model and investigate the behavior of traffic flow in two routes, providing various types of information to a limited number of traveling particles according to the share rate of information. To analytically understand the phenomena, we develop a coarse-grained representation of the model. By analyzing the model, we find the most effective types of information to minimize particles' travel time and the existence of an optimal share rate according to route conditions. PMID:26172765

  6. NORTH FORK SMITH RIVER ROADLESS AREA, CALIFORNIA AND OREGON.

    USGS Publications Warehouse

    Gray, Floyd; Hamilton, Michael

    1984-01-01

    Geologic, geochemical, and geophysical investigations and a survey of mines and prospects were conducted to evaluate the mineral-resource potential of the North Fork Smith River Roadless Area, California. The area has probable and sustantiated resource potential for nickel, chromium, copper, and mercury and approximately 2300 mining claims exist in or adjacent to the area. The geologic terrane precludes the occurrence of fossil fuel resources.

  7. Stalled replication forks within heterochromatin require ATRX for protection

    PubMed Central

    Huh, M S; Ivanochko, D; Hashem, L E; Curtin, M; Delorme, M; Goodall, E; Yan, K; Picketts, D J

    2016-01-01

    Expansive growth of neural progenitor cells (NPCs) is a prerequisite to the temporal waves of neuronal differentiation that generate the six-layered neocortex, while also placing a heavy burden on proteins that regulate chromatin packaging and genome integrity. This problem is further reflected by the growing number of developmental disorders caused by mutations in chromatin regulators. ATRX gene mutations cause a severe intellectual disability disorder (α-thalassemia mental retardation X-linked (ATRX) syndrome; OMIM no. 301040), characterized by microcephaly, urogenital abnormalities and α-thalassemia. Although the ATRX protein is required for the maintenance of repetitive DNA within heterochromatin, how this translates to disease pathogenesis remain poorly understood and was a focus of this study. We demonstrate that AtrxFoxG1Cre forebrain-specific conditional knockout mice display poly(ADP-ribose) polymerase-1 (Parp-1) hyperactivation during neurogenesis and generate fewer late-born Cux1- and Brn2-positive neurons that accounts for the reduced cortical size. Moreover, DNA damage, induced Parp-1 and Atm activation is elevated in progenitor cells and contributes to their increased level of cell death. ATRX-null HeLa cells are similarly sensitive to hydroxyurea-induced replication stress, accumulate DNA damage and proliferate poorly. Impaired BRCA1-RAD51 colocalization and PARP-1 hyperactivation indicated that stalled replication forks are not efficiently protected. DNA fiber assays confirmed that MRE11 degradation of stalled replication forks was rampant in the absence of ATRX or DAXX. Indeed, fork degradation in ATRX-null cells could be attenuated by treatment with the MRE11 inhibitor mirin, or exacerbated by inhibiting PARP-1 activity. Taken together, these results suggest that ATRX is required to limit replication stress during cellular proliferation, whereas upregulation of PARP-1 activity functions as a compensatory mechanism to protect stalled forks

  8. Stalled replication forks within heterochromatin require ATRX for protection.

    PubMed

    Huh, M S; Ivanochko, D; Hashem, L E; Curtin, M; Delorme, M; Goodall, E; Yan, K; Picketts, D J

    2016-05-12

    Expansive growth of neural progenitor cells (NPCs) is a prerequisite to the temporal waves of neuronal differentiation that generate the six-layered neocortex, while also placing a heavy burden on proteins that regulate chromatin packaging and genome integrity. This problem is further reflected by the growing number of developmental disorders caused by mutations in chromatin regulators. ATRX gene mutations cause a severe intellectual disability disorder (α-thalassemia mental retardation X-linked (ATRX) syndrome; OMIM no. 301040), characterized by microcephaly, urogenital abnormalities and α-thalassemia. Although the ATRX protein is required for the maintenance of repetitive DNA within heterochromatin, how this translates to disease pathogenesis remain poorly understood and was a focus of this study. We demonstrate that Atrx(FoxG1Cre) forebrain-specific conditional knockout mice display poly(ADP-ribose) polymerase-1 (Parp-1) hyperactivation during neurogenesis and generate fewer late-born Cux1- and Brn2-positive neurons that accounts for the reduced cortical size. Moreover, DNA damage, induced Parp-1 and Atm activation is elevated in progenitor cells and contributes to their increased level of cell death. ATRX-null HeLa cells are similarly sensitive to hydroxyurea-induced replication stress, accumulate DNA damage and proliferate poorly. Impaired BRCA1-RAD51 colocalization and PARP-1 hyperactivation indicated that stalled replication forks are not efficiently protected. DNA fiber assays confirmed that MRE11 degradation of stalled replication forks was rampant in the absence of ATRX or DAXX. Indeed, fork degradation in ATRX-null cells could be attenuated by treatment with the MRE11 inhibitor mirin, or exacerbated by inhibiting PARP-1 activity. Taken together, these results suggest that ATRX is required to limit replication stress during cellular proliferation, whereas upregulation of PARP-1 activity functions as a compensatory mechanism to protect stalled forks

  9. NORTH FORK OF THE AMERICAN RIVER WILDERNESS STUDY AREA, CALIFORNIA.

    USGS Publications Warehouse

    Harwood, David S.; Federspiel, Francis E.

    1984-01-01

    Mineral-resource surveys of the North Fork of the American River Wilderness study area, California have identified a zone of substantiated resource potential for gold and silver. Zones of probable gold and silver potential occur in the eastern part of the area between the Wubbena and La Trinidad mines and locally around the Marrs mine. A zone with probable chromium potential occurs in the serpentinite belt along the western border of the area. No energy resources were identified in this study.

  10. Supercoiling, knotting and replication fork reversal in partially replicated plasmids

    PubMed Central

    Olavarrieta, L.; Martínez-Robles, M. L.; Sogo, J. M.; Stasiak, A.; Hernández, P.; Krimer, D. B.; Schvartzman, J. B.

    2002-01-01

    To study the structure of partially replicated plasmids, we cloned the Escherichia coli polar replication terminator TerE in its active orientation at different locations in the ColE1 vector pBR18. The resulting plasmids, pBR18-TerE@StyI and pBR18-TerE@EcoRI, were analyzed by neutral/neutral two-dimensional agarose gel electrophoresis and electron microscopy. Replication forks stop at the Ter–TUS complex, leading to the accumulation of specific replication intermediates with a mass 1.26 times the mass of non-replicating plasmids for pBR18-TerE@StyI and 1.57 times for pBR18-TerE@EcoRI. The number of knotted bubbles detected after digestion with ScaI and the number and electrophoretic mobility of undigested partially replicated topoisomers reflect the changes in plasmid topology that occur in DNA molecules replicated to different extents. Exposure to increasing concentrations of chloroquine or ethidium bromide revealed that partially replicated topoisomers (CCCRIs) do not sustain positive supercoiling as efficiently as their non-replicating counterparts. It was suggested that this occurs because in partially replicated plasmids a positive ΔLk is absorbed by regression of the replication fork. Indeed, we showed by electron microscopy that, at least in the presence of chloroquine, some of the CCCRIs of pBR18-Ter@StyI formed Holliday-like junction structures characteristic of reversed forks. However, not all the positive supercoiling was absorbed by fork reversal in the presence of high concentrations of ethidium bromide. PMID:11809877

  11. Molecular clock fork phylogenies: closed form analytic maximum likelihood solutions.

    PubMed

    Chor, Benny; Snir, Sagi

    2004-12-01

    Maximum likelihood (ML) is increasingly used as an optimality criterion for selecting evolutionary trees, but finding the global optimum is a hard computational task. Because no general analytic solution is known, numeric techniques such as hill climbing or expectation maximization (EM) are used in order to find optimal parameters for a given tree. So far, analytic solutions were derived only for the simplest model-three-taxa, two-state characters, under a molecular clock. Quoting Ziheng Yang, who initiated the analytic approach,"this seems to be the simplest case, but has many of the conceptual and statistical complexities involved in phylogenetic estimation."In this work, we give general analytic solutions for a family of trees with four-taxa, two-state characters, under a molecular clock. The change from three to four taxa incurs a major increase in the complexity of the underlying algebraic system, and requires novel techniques and approaches. We start by presenting the general maximum likelihood problem on phylogenetic trees as a constrained optimization problem, and the resulting system of polynomial equations. In full generality, it is infeasible to solve this system, therefore specialized tools for the molecular clock case are developed. Four-taxa rooted trees have two topologies-the fork (two subtrees with two leaves each) and the comb (one subtree with three leaves, the other with a single leaf). We combine the ultrametric properties of molecular clock fork trees with the Hadamard conjugation to derive a number of topology dependent identities. Employing these identities, we substantially simplify the system of polynomial equations for the fork. We finally employ symbolic algebra software to obtain closed formanalytic solutions (expressed parametrically in the input data). In general, four-taxa trees can have multiple ML points. In contrast, we can now prove that each fork topology has a unique(local and global) ML point.

  12. Waste management plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    SciTech Connect

    1996-04-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Plant Landfill V, and restore the affected floodplain. The waste management plan addresses management and disposition of all wastes generated during the LEFPC remedial action. Most of the solid wastes will be sanitary or construction/demolition wastes and will be disposed of at existing Y- 12 facilities. Some small amounts of hazardous waste are anticipated, along with possible low-level or mixed wastes (> 35 pCi/g). Liquid wastes will be generated which will be sanitary and capable of being disposed of at the Oak Ridge Sewage Treatment Plant, except sanitary sewage.

  13. Replication fork stability confers chemoresistance in BRCA-deficient cells.

    PubMed

    Ray Chaudhuri, Arnab; Callen, Elsa; Ding, Xia; Gogola, Ewa; Duarte, Alexandra A; Lee, Ji-Eun; Wong, Nancy; Lafarga, Vanessa; Calvo, Jennifer A; Panzarino, Nicholas J; John, Sam; Day, Amanda; Crespo, Anna Vidal; Shen, Binghui; Starnes, Linda M; de Ruiter, Julian R; Daniel, Jeremy A; Konstantinopoulos, Panagiotis A; Cortez, David; Cantor, Sharon B; Fernandez-Capetillo, Oscar; Ge, Kai; Jonkers, Jos; Rottenberg, Sven; Sharan, Shyam K; Nussenzweig, André

    2016-07-21

    Cells deficient in the Brca1 and Brca2 genes have reduced capacity to repair DNA double-strand breaks by homologous recombination and consequently are hypersensitive to DNA-damaging agents, including cisplatin and poly(ADP-ribose) polymerase (PARP) inhibitors. Here we show that loss of the MLL3/4 complex protein, PTIP, protects Brca1/2-deficient cells from DNA damage and rescues the lethality of Brca2-deficient embryonic stem cells. However, PTIP deficiency does not restore homologous recombination activity at double-strand breaks. Instead, its absence inhibits the recruitment of the MRE11 nuclease to stalled replication forks, which in turn protects nascent DNA strands from extensive degradation. More generally, acquisition of PARP inhibitors and cisplatin resistance is associated with replication fork protection in Brca2-deficient tumour cells that do not develop Brca2 reversion mutations. Disruption of multiple proteins, including PARP1 and CHD4, leads to the same end point of replication fork protection, highlighting the complexities by which tumour cells evade chemotherapeutic interventions and acquire drug resistance. PMID:27443740

  14. Split quartz tuning fork sensors for enhanced sensitivity force detection

    NASA Astrophysics Data System (ADS)

    Labardi, M.; Lucchesi, M.

    2015-03-01

    Quartz tuning forks (TFs) are often employed in dynamic-mode atomic force microscopy (AFM) as piezoelectric force sensors, to replace the usual AFM microcantilevers, especially in ultra-high vacuum or cryogenic environments. A sharp tip is attached to one of the fork prongs, to obtain atomic scale AFM resolution. We devise a novel TF design by splitting the electrodes of its two prongs, which are produced at the factory as connected to each other, in order to address each of them separately. In such way, the motion of the probe tip can be unambiguously measured, irrespective of the motion of the other prong, which conversely influences its measurement in standard TFs. Furthermore, attachment of the probe tip dramatically spoils the oscillator Q-factor, as it unbalances the two prongs of the TF, with consequent dissipation of energy through the fork holder, due to the motion of the center of mass (CM) of the system. The possibility to independently drive the two prongs of the split TF gives the opportunity to rebalance them just by electrical means, thereby restoring the original Q-factor, by stopping the CM motion. By modeling the split TF as a three-mass, four-spring system, its behavior can be accurately described. Our model is used to explore alternative operation modes with enhanced sensitivity to applied forces.

  15. Mitochondrial transcription termination factor 1 directs polar replication fork pausing.

    PubMed

    Shi, Yonghong; Posse, Viktor; Zhu, Xuefeng; Hyvärinen, Anne K; Jacobs, Howard T; Falkenberg, Maria; Gustafsson, Claes M

    2016-07-01

    During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can cause replication fork collapse and genomic instability. To avoid this problem, a replication fork barrier protein is situated downstream of rDNA, there preventing replication in the direction opposite rDNA transcription. A potential candidate for a similar function in mitochondria is the mitochondrial transcription termination factor 1 (MTERF1, also denoted mTERF), which binds to a sequence just downstream of the ribosomal transcription unit. Previous studies have shown that MTERF1 prevents antisense transcription over the ribosomal RNA genes, a process which we here show to be independent of the transcription elongation factor TEFM. Importantly, we now demonstrate that MTERF1 arrests mitochondrial DNA (mtDNA) replication with distinct polarity. The effect is explained by the ability of MTERF1 to act as a directional contrahelicase, blocking mtDNA unwinding by the mitochondrial helicase TWINKLE. This conclusion is also supported by in vivo evidence that MTERF1 stimulates TWINKLE pausing. We conclude that MTERF1 can direct polar replication fork arrest in mammalian mitochondria. PMID:27112570

  16. Protection or resection: BOD1L as a novel replication fork protection factor.

    PubMed

    Higgs, Martin R; Stewart, Grant S

    2016-01-01

    Replication stress, defined as the slowing or stalling of cellular DNA replication forks, represents a serious threat to genome stability. Numerous cellular pathways protect against replication stress and maintain genomic integrity. Among these, the Fanconi Anemia/homologous recombination pathways are critical for recognizing and repairing stalled replication forks. Members of these pathways play a vital role in protecting damaged forks from uncontrolled attack from cellular nucleases, which would otherwise render these irreparable. Recent studies have begun to shed light on the protective factors necessary to suppress nucleolytic over-processing of nascent DNA, and on the different cellular nucleases involved. Here, we review our recent identification of a novel fork protection factor, BOD1L, and discuss its role in preventing the processing of stalled replication forks within the context of current knowledge of the replication fork 'protectosome'.

  17. RecA protein promotes the regression of stalled replication forks in vitro.

    PubMed

    Robu, M E; Inman, R B; Cox, M M

    2001-07-17

    Replication forks are halted by many types of DNA damage. At the site of a leading-strand DNA lesion, forks may stall and leave the lesion in a single-strand gap. Fork regression is the first step in several proposed pathways that permit repair without generating a double-strand break. Using model DNA substrates designed to mimic one of the known structures of a fork stalled at a leading-strand lesion, we show here that RecA protein of Escherichia coli will promote a fork regression reaction in vitro. The regression process exhibits an absolute requirement for ATP hydrolysis and is enhanced when dATP replaces ATP. The reaction is not affected by the inclusion of the RecO and R proteins. We present this reaction as one of several potential RecA protein roles in the repair of stalled and/or collapsed replication forks in bacteria. PMID:11459955

  18. Coordinated protein and DNA remodeling by human HLTF on stalled replication fork.

    PubMed

    Achar, Yathish Jagadheesh; Balogh, David; Haracska, Lajos

    2011-08-23

    Human helicase-like transcription factor (HLTF) exhibits ubiquitin ligase activity for proliferating cell nuclear antigen (PCNA) polyubiquitylation as well as double-stranded DNA translocase activity for remodeling stalled replication fork by fork reversal, which can support damage bypass by template switching. However, a stalled replication fork is surrounded by various DNA-binding proteins which can inhibit the access of damage bypass players, and it is unknown how these proteins become displaced. Here we reveal that HLTF has an ATP hydrolysis-dependent protein remodeling activity, by which it can remove proteins bound to the replication fork. Moreover, we demonstrate that HLTF can displace a broad spectrum of proteins such as replication protein A (RPA), PCNA, and replication factor C (RFC), thereby providing the first example for a protein clearing activity at the stalled replication fork. Our findings clarify how remodeling of a stalled replication fork can occur if it is engaged in interactions with masses of proteins.

  19. Calibrating a tuning fork for use as a scanning probe microscope force sensor

    SciTech Connect

    Qin Yexian; Reifenberger, R.

    2007-06-15

    Quartz tuning forks mounted with sharp tips provide an alternate method to silicon microcantilevers for probing the tip-substrate interaction in scanning probe microscopy. The high quality factor and stable resonant frequency of the tuning fork allow accurate measurements of small shifts in the resonant frequency as the tip approaches the substrate. To permit an accurate measure of surface interaction forces, the electrical and piezoelectromechanical properties of a tuning fork have been characterized using a fiber optical interferometer.

  20. Recovery of arrested replication forks by homologous recombination is error-prone.

    PubMed

    Iraqui, Ismail; Chekkal, Yasmina; Jmari, Nada; Pietrobon, Violena; Fréon, Karine; Costes, Audrey; Lambert, Sarah A E

    2012-01-01

    Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability. Here, we report that recovery of impeded replication forks by homologous recombination is error-prone. Using a fork-arrest-based assay in fission yeast, we demonstrate that a single collapsed fork can cause mutations and large-scale genomic changes, including deletions and translocations. Fork-arrest-induced gross chromosomal rearrangements are mediated by inappropriate ectopic recombination events at the site of collapsed forks. Inverted repeats near the site of fork collapse stimulate large-scale genomic changes up to 1,500 times over spontaneous events. We also show that the high accuracy of DNA replication during S-phase is impaired by impediments to fork progression, since fork-arrest-induced mutation is due to erroneous DNA synthesis during recovery of replication forks. The mutations caused are small insertions/duplications between short tandem repeats (micro-homology) indicative of replication slippage. Our data establish that collapsed forks, but not stalled forks, recovered by homologous recombination are prone to replication slippage. The inaccuracy of DNA synthesis does not rely on PCNA ubiquitination or trans-lesion-synthesis DNA polymerases, and it is not counteracted by mismatch repair. We propose that deletions/insertions, mediated by micro-homology, leading to copy number variations during replication stress may arise by progression of error-prone replication forks restarted by homologous recombination. PMID:23093942

  1. Some hydrologic aspects of proposed coal mining in the north fork of the Flathead River headwaters area, northwest Montana and southeast British Columbia. Completion report

    SciTech Connect

    Noble, R.A.; Van Voast, W.A.; Sonderegger, J.L.

    1984-12-01

    The proposed Cabin Creek coal mine in Canada, just north of the Montana-British Columbia border, has caused considerable concern in Montana because of its potential impact on the North Fork of the Flathead River. The objective of the project was to assess the impact of mining activity at the Cabin Creek site on water quality in the Flathead River. The results of the project indicate that acid mine drainage from the Cabin Creek mine should not be a problem. Analysis of the coal samples indicated that the acid-neutralizing potential exceeded the acid producing potential. Mineralization of waters entering the North Fork could result in some degradation of water quality, as could sediment from soil disturbances. The extent of degradation will depend on mining practices and on the location of retention facilities for water and other residual materials extracted from the mine.

  2. Strand-specific analysis shows protein binding at replication forks and PCNA unloading from lagging strands when forks stall.

    PubMed

    Yu, Chuanhe; Gan, Haiyun; Han, Junhong; Zhou, Zhi-Xiong; Jia, Shaodong; Chabes, Andrei; Farrugia, Gianrico; Ordog, Tamas; Zhang, Zhiguo

    2014-11-20

    In eukaryotic cells, DNA replication proceeds with continuous synthesis of leading-strand DNA and discontinuous synthesis of lagging-strand DNA. Here we describe a method, eSPAN (enrichment and sequencing of protein-associated nascent DNA), which reveals the genome-wide association of proteins with leading and lagging strands of DNA replication forks. Using this approach in budding yeast, we confirm the strand specificities of DNA polymerases delta and epsilon and show that the PCNA clamp is enriched at lagging strands compared with leading-strand replication. Surprisingly, at stalled forks, PCNA is unloaded specifically from lagging strands. PCNA unloading depends on the Elg1-containing alternative RFC complex, ubiquitination of PCNA, and the checkpoint kinases Mec1 and Rad53. Cells deficient in PCNA unloading exhibit increased chromosome breaks. Our studies provide a tool for studying replication-related processes and reveal a mechanism whereby checkpoint kinases regulate strand-specific unloading of PCNA from stalled replication forks to maintain genome stability.

  3. Mammalian RAD51 paralogs protect nascent DNA at stalled forks and mediate replication restart.

    PubMed

    Somyajit, Kumar; Saxena, Sneha; Babu, Sharath; Mishra, Anup; Nagaraju, Ganesh

    2015-11-16

    Mammalian RAD51 paralogs are implicated in the repair of collapsed replication forks by homologous recombination. However, their physiological roles in replication fork maintenance prior to fork collapse remain obscure. Here, we report on the role of RAD51 paralogs in short-term replicative stress devoid of DSBs. We show that RAD51 paralogs localize to nascent DNA and common fragile sites upon replication fork stalling. Strikingly, RAD51 paralogs deficient cells exhibit elevated levels of 53BP1 nuclear bodies and increased DSB formation, the latter being attributed to extensive degradation of nascent DNA at stalled forks. RAD51C and XRCC3 promote the restart of stalled replication in an ATP hydrolysis dependent manner by disengaging RAD51 and other RAD51 paralogs from the halted forks. Notably, we find that Fanconi anemia (FA)-like disorder and breast and ovarian cancer patient derived mutations of RAD51C fails to protect replication fork, exhibit under-replicated genomic regions and elevated micro-nucleation. Taken together, RAD51 paralogs prevent degradation of stalled forks and promote the restart of halted replication to avoid replication fork collapse, thereby maintaining genomic integrity and suppressing tumorigenesis.

  4. THE FORK AND THE KINASE: A DNA REPLICATION TALE FROM A CHK1 PERSPECTIVE

    PubMed Central

    González Besteiro, Marina A.; Gottifredi, Vanesa

    2014-01-01

    Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. The checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged-DNA. Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Such findings unveil a puzzling connection between Chk1 and DNA-lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, the multifaceted and versatile functions of Chk1 at ongoing forks and replication origins determine the extent and quality of the cellular response to replication stress. PMID:25795119

  5. Chk1 requirement for high global rates of replication fork progression during normal vertebrate S phase.

    PubMed

    Petermann, Eva; Maya-Mendoza, Apolinar; Zachos, George; Gillespie, David A F; Jackson, Dean A; Caldecott, Keith W

    2006-04-01

    Chk1 protein kinase maintains replication fork stability in metazoan cells in response to DNA damage and DNA replication inhibitors. Here, we have employed DNA fiber labeling to quantify, for the first time, the extent to which Chk1 maintains global replication fork rates during normal vertebrate S phase. We report that replication fork rates in Chk1(-/-) chicken DT40 cells are on average half of those observed with wild-type cells. Similar results were observed if Chk1 was inhibited or depleted in wild-type DT40 cells or HeLa cells by incubation with Chk1 inhibitor or small interfering RNA. In addition, reduced rates of fork extension were observed with permeabilized Chk1(-/-) cells in vitro. The requirement for Chk1 for high fork rates during normal S phase was not to suppress promiscuous homologous recombination at replication forks, because inhibition of Chk1 similarly slowed fork progression in XRCC3(-/-) DT40 cells. Rather, we observed an increased number of replication fibers in Chk1(-/-) cells in which the nascent strand is single-stranded, supporting the idea that slow global fork rates in unperturbed Chk1(-/-) cells are associated with the accumulation of aberrant replication fork structures.

  6. Evaluation of Thompson-type trend and monthly weather data models for corn yields in Iowa, Illinois, and Indiana

    NASA Technical Reports Server (NTRS)

    French, V. (Principal Investigator)

    1982-01-01

    An evaluation was made of Thompson-Type models which use trend terms (as a surrogate for technology), meteorological variables based on monthly average temperature, and total precipitation to forecast and estimate corn yields in Iowa, Illinois, and Indiana. Pooled and unpooled Thompson-type models were compared. Neither was found to be consistently superior to the other. Yield reliability indicators show that the models are of limited use for large area yield estimation. The models are objective and consistent with scientific knowledge. Timely yield forecasts and estimates can be made during the growing season by using normals or long range weather forecasts. The models are not costly to operate and are easy to use and understand. The model standard errors of prediction do not provide a useful current measure of modeled yield reliability.

  7. Digital geologic map of part of the Thompson Falls 1:100,000 quadrangle, Idaho

    USGS Publications Warehouse

    Lewis, Reed S.; Derkey, Pamela D.

    1999-01-01

    The geology of the Thompson Falls 1:100,000 quadrangle, Idaho was compiled by Reed S. Lewis in 1997 onto a 1:100,000-scale greenline mylar of the topographic base map for input into a geographic information system (GIS). The resulting digital geologic map GIS can be queried in many ways to produce a variety of geologic maps. Digital base map data files (topography, roads, towns, rivers and lakes, etc.) are not included: they may be obtained from a variety of commercial and government sources. This database is not meant to be used or displayed at any scale larger than 1:100,000 (e.g., 1:62,500 or 1:24,000). The map area is located in north Idaho. This open-file report describes the geologic map units, the methods used to convert the geologic map data into a digital format, the Arc/Info GIS file structures and relationships, and explains how to download the digital files from the U.S. Geological Survey public access World Wide Web site on the Internet.

  8. A celebration of mechanics: from nano to macro. The J. Michael T. Thompson Festschrift issue.

    PubMed

    Elishakoff, Isaac

    2013-06-28

    This Theme Issue is dedicated to the topic 'Mechanics: from nano to macro' and marks the 75th birthday of Dr J. Michael T. Thompson, Fellow of the Royal Society, whose current affiliations are as follows: (i) Honorary Fellow, Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge; (ii) Emeritus Professor of Nonlinear Dynamics, Department of Civil, Environmental and Geomatic Engineering, University College London; and (iii) Professor of Theoretical and Applied Dynamics (Distinguished Sixth Century Chair, part-time), University of Aberdeen. He also serves as Chairman of the Board of Directors at ES-Consult (consulting engineers) in Copenhagen, Denmark. The pertinent question that arises from the very start is: should we first salute Michael and then describe the Theme Issue, or vice versa? Indeed, according to Blaise Pascal (1623-1662), the last thing one discovers in composing a work is what to put first. I would like to take the liberty of deviating from the tradition of the Philosophical Transactions and start with the tribute to Michael; after all he is the prime cause of this Theme Issue.

  9. BRCA1 controls homologous recombination at Tus/Ter-stalled mammalian replication forks.

    PubMed

    Willis, Nicholas A; Chandramouly, Gurushankar; Huang, Bin; Kwok, Amy; Follonier, Cindy; Deng, Chuxia; Scully, Ralph

    2014-06-26

    Replication fork stalling can promote genomic instability, predisposing to cancer and other diseases. Stalled replication forks may be processed by sister chromatid recombination (SCR), generating error-free or error-prone homologous recombination (HR) outcomes. In mammalian cells, a long-standing hypothesis proposes that the major hereditary breast/ovarian cancer predisposition gene products, BRCA1 and BRCA2, control HR/SCR at stalled replication forks. Although BRCA1 and BRCA2 affect replication fork processing, direct evidence that BRCA gene products regulate homologous recombination at stalled chromosomal replication forks is lacking, due to a dearth of tools for studying this process. Here we report that the Escherichia coli Tus/Ter complex can be engineered to induce site-specific replication fork stalling and chromosomal HR/SCR in mouse cells. Tus/Ter-induced homologous recombination entails processing of bidirectionally arrested forks. We find that the Brca1 carboxy (C)-terminal tandem BRCT repeat and regions of Brca1 encoded by exon 11-two Brca1 elements implicated in tumour suppression-control Tus/Ter-induced homologous recombination. Inactivation of either Brca1 or Brca2 increases the absolute frequency of 'long-tract' gene conversions at Tus/Ter-stalled forks, an outcome not observed in response to a site-specific endonuclease-mediated chromosomal double-strand break. Therefore, homologous recombination at stalled forks is regulated differently from homologous recombination at double-strand breaks arising independently of a replication fork. We propose that aberrant long-tract homologous recombination at stalled replication forks contributes to genomic instability and breast/ovarian cancer predisposition in BRCA mutant cells.

  10. Hydroxyurea-stalled replication forks become progressively inactivated and require two different RAD51-mediated pathways for restart and repair.

    PubMed

    Petermann, Eva; Orta, Manuel Luís; Issaeva, Natalia; Schultz, Niklas; Helleday, Thomas

    2010-02-26

    Faithful DNA replication is essential to all life. Hydroxyurea (HU) depletes the cells of dNTPs, which initially results in stalled replication forks that, after prolonged treatment, collapse into DSBs. Here, we report that stalled replication forks are efficiently restarted in a RAD51-dependent process that does not trigger homologous recombination (HR). The XRCC3 protein, which is required for RAD51 foci formation, is also required for replication restart of HU-stalled forks, suggesting that RAD51-mediated strand invasion supports fork restart. In contrast, replication forks collapsed by prolonged replication blocks do not restart, and global replication is rescued by new origin firing. We find that RAD51-dependent HR is triggered for repair of collapsed replication forks, without apparent restart. In conclusion, our data suggest that restart of stalled replication forks and HR repair of collapsed replication forks require two distinct RAD51-mediated pathways.

  11. 77 FR 42761 - Final Environmental Impact Statement for the Oil and Gas Management Plan at Big South Fork...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... Fork National River and Recreation Area and Obed Wild and Scenic River, Tennessee and Kentucky AGENCY... Blount, Big South Fork National River ] and Recreation Area and Obed Wild and Scenic River Chief...

  12. Acoustic Resonances in Helium Fluids Excited by Quartz Tuning Forks

    NASA Astrophysics Data System (ADS)

    Salmela, A.; Tuoriniemi, J.; Rysti, J.

    2011-03-01

    Ordinary quartz tuning fork resonators, operated at about 30 or 200 kHz frequency, couple to acoustic first and second sound resonances in helium fluids under certain conditions. We have studied acoustic resonances in supercritical 4He, normal and superfluid 4He, and in isotopic mixtures of helium. Suggestive temperature, pressure, and concentration dependences are given. Furthermore, we propose a thermometric reference point device based on second sound resonances in helium mixtures, and indicate possible differences in the nature of second sound resonances in superfluid 4He and helium mixtures.

  13. MIDDLE FORK OF THE JUDITH RIVER WILDERNESS STUDY AREA, MONTANA.

    USGS Publications Warehouse

    Reynolds, Mitchell W.; Hamilton, Michael

    1984-01-01

    The Middle Fork of the Judith River Wilderness Study Area in Montana has several prospects containing local small inferred resources and several exposed mineral occurrences bearing silver, lead, copper, and gold along its north and west edges with no identified resource potential. Geologic, geophysical, and geochemical studies, conducted to appraise the mineral resources of the study area, identified no other anomalous concentrations of metallic elements. A gem-quality sapphire resource, present near the east boundary, cannot be identified within the study area. The area holds little promise for the occurrence of other mineral or energy resources.

  14. BLACK FORK MOUNTAIN ROADLESS AREA, ARAKANSAS AND OKLAHOMA.

    USGS Publications Warehouse

    Miller, Mary H.

    1984-01-01

    Black Fork Mountain Roadless Area covers about 21 sq mi in the Ouachita National Forest in Polk County, Arkansas and LeFlore County, Oklahoma. On the basis of a mineral survey the area has little promise for the occurrence of metallic mineral resources. Stone and sand and gravel suitable for construction purposes occur in the Jackfork Sandstone and the Stanley Shale which also occur outside the roadless area. Although the potential for gas and oil is unknown and no resource potential was identified, some investigators believe that there is a possibility for the occurrence of gas and oil in the roadless area.

  15. UV stalled replication forks restart by re-priming in human fibroblasts.

    PubMed

    Elvers, Ingegerd; Johansson, Fredrik; Groth, Petra; Erixon, Klaus; Helleday, Thomas

    2011-09-01

    Restarting stalled replication forks is vital to avoid fatal replication errors. Previously, it was demonstrated that hydroxyurea-stalled replication forks rescue replication either by an active restart mechanism or by new origin firing. To our surprise, using the DNA fibre assay, we only detect a slightly reduced fork speed on a UV-damaged template during the first hour after UV exposure, and no evidence for persistent replication fork arrest. Interestingly, no evidence for persistent UV-induced fork stalling was observed even in translesion synthesis defective, Polη(mut) cells. In contrast, using an assay to measure DNA molecule elongation at the fork, we observe that continuous DNA elongation is severely blocked by UV irradiation, particularly in UV-damaged Polη(mut) cells. In conclusion, our data suggest that UV-blocked replication forks restart effectively through re-priming past the lesion, leaving only a small gap opposite the lesion. This allows continuation of replication on damaged DNA. If left unfilled, the gaps may collapse into DNA double-strand breaks that are repaired by a recombination pathway, similar to the fate of replication forks collapsed after hydroxyurea treatment.

  16. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Altus Dam and Reservoir, North Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork Red River, Okla. The Bureau of Reclamation, or its designated agent, shall operate the...

  17. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Altus Dam and Reservoir, North Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork Red River, Okla. The Bureau of Reclamation, or its designated agent, shall operate the...

  18. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Altus Dam and Reservoir, North Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork Red River, Okla. The Bureau of Reclamation, or its designated agent, shall operate the...

  19. Analysis of protein dynamics at active, stalled, and collapsed replication forks.

    PubMed

    Sirbu, Bianca M; Couch, Frank B; Feigerle, Jordan T; Bhaskara, Srividya; Hiebert, Scott W; Cortez, David

    2011-06-15

    Successful DNA replication and packaging of newly synthesized DNA into chromatin are essential to maintain genome integrity. Defects in the DNA template challenge genetic and epigenetic inheritance. Unfortunately, tracking DNA damage responses (DDRs), histone deposition, and chromatin maturation at replication forks is difficult in mammalian cells. Here we describe a technology called iPOND (isolation of proteins on nascent DNA) to analyze proteins at active and damaged replication forks at high resolution. Using this methodology, we define the timing of histone deposition and chromatin maturation. Class 1 histone deacetylases are enriched at replisomes and remove predeposition marks on histone H4. Chromatin maturation continues even when decoupled from replisome movement. Furthermore, fork stalling causes changes in the recruitment and phosphorylation of proteins at the damaged fork. Checkpoint kinases catalyze H2AX phosphorylation, which spreads from the stalled fork to include a large chromatin domain even prior to fork collapse and double-strand break formation. Finally, we demonstrate a switch in the DDR at persistently stalled forks that includes MRE11-dependent RAD51 assembly. These data reveal a dynamic recruitment of proteins and post-translational modifications at damaged forks and surrounding chromatin. Furthermore, our studies establish iPOND as a useful methodology to study DNA replication and chromatin maturation.

  20. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  1. Origin & Evolution of the Grand Forks Human Nutrition Research Center, 1970-90

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the early 1960s William E Cornatzer, MD, PhD, suggested the need for increased USDA research concerning human nutrition, and creation of the Grand Forks Human Nutrition Laboratory (Grand Forks Human Nutrition Research Center). He shared ideas with Senator Milton R. Young of North Dakota, who requ...

  2. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Altus Dam and Reservoir, North Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork Red River, Okla. The Bureau of Reclamation, or its designated agent, shall operate the...

  3. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  4. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  5. Replication forks blocked by protein-DNA complexes have limited stability in vitro.

    PubMed

    McGlynn, Peter; Guy, Colin P

    2008-08-29

    There are many barriers that replication forks must overcome in order to duplicate a genome in vivo. These barriers include damage to the template DNA and proteins bound to this template. If replication is halted by such a block, then the block must be either removed or bypassed for replication to continue. If continuation of replication employs the original fork, avoiding the need to reload the replication apparatus, then the blocked replisome must retain functionality. In vivo studies of Escherichia coli replication forks suggest that replication forks blocked by protein-DNA complexes retain the ability to resume replication upon removal of the block for several hours. Here we tested the functional stability of replication forks reconstituted in vitro and blocked by lac repressor-operator complexes. Once a fork comes to a halt at such a block, it cannot continue subsequently to translocate through the block until addition of IPTG induces repressor dissociation. However, the ability to resume replication is retained only for 4-6 min regardless of the topological state of the template DNA. Comparison of our in vitro data with previous in vivo data suggests that either accessory factors that stabilise blocked forks are present in vivo or the apparent stability of blocked forks in vivo is due to continual reloading of the replication apparatus at the site of the block.

  6. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  7. 77 FR 66541 - Safety Zone; Alliance Road Bridge Demolition; Black Warrior River, Locust Fork; Birmingham, AL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-06

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice... Warrior River, Locust Fork; Birmingham, AL AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone for a portion of the Locust Fork to the...

  8. The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing.

    PubMed

    Hu, Jiazhi; Sun, Lei; Shen, Fenfen; Chen, Yufei; Hua, Yu; Liu, Yang; Zhang, Mian; Hu, Yiren; Wang, Qingsong; Xu, Wei; Sun, Fei; Ji, Jianguo; Murray, Johanne M; Carr, Antony M; Kong, Daochun

    2012-06-01

    When replication forks stall at damaged bases or upon nucleotide depletion, the intra-S phase checkpoint ensures they are stabilized and can restart. In intra-S checkpoint-deficient budding yeast, stalling forks collapse, and ∼10% form pathogenic chicken foot structures, contributing to incomplete replication and cell death (Lopes et al., 2001; Sogo et al., 2002; Tercero and Diffley, 2001). Using fission yeast, we report that the Cds1(Chk2) effector kinase targets Dna2 on S220 to regulate, both in vivo and in vitro, Dna2 association with stalled replication forks in chromatin. We demonstrate that Dna2-S220 phosphorylation and the nuclease activity of Dna2 are required to prevent fork reversal. Consistent with this, Dna2 can efficiently cleave obligate precursors of fork regression-regressed leading or lagging strands-on model replication forks. We propose that Dna2 cleavage of regressed nascent strands prevents fork reversal and thus stabilizes stalled forks to maintain genome stability during replication stress.

  9. Substrate-selective repair and restart of replication forks by DNA translocases.

    PubMed

    Bétous, Rémy; Couch, Frank B; Mason, Aaron C; Eichman, Brandt F; Manosas, Maria; Cortez, David

    2013-06-27

    Stalled replication forks are sources of genetic instability. Multiple fork-remodeling enzymes are recruited to stalled forks, but how they work to promote fork restart is poorly understood. By combining ensemble biochemical assays and single-molecule studies with magnetic tweezers, we show that SMARCAL1 branch migration and DNA-annealing activities are directed by the single-stranded DNA-binding protein RPA to selectively regress stalled replication forks caused by blockage to the leading-strand polymerase and to restore normal replication forks with a lagging-strand gap. We unveil the molecular mechanisms by which RPA enforces SMARCAL1 substrate preference. E. coli RecG acts similarly to SMARCAL1 in the presence of E. coli SSB, whereas the highly related human protein ZRANB3 has different substrate preferences. Our findings identify the important substrates of SMARCAL1 in fork repair, suggest that RecG and SMARCAL1 are functional orthologs, and provide a comprehensive model of fork repair by these DNA translocases.

  10. 33 CFR 208.26 - Altus Dam and Reservoir, North Fork Red River, Okla.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Altus Dam and Reservoir, North Fork Red River, Okla. 208.26 Section 208.26 Navigation and Navigable Waters CORPS OF ENGINEERS..., North Fork Red River, Okla. The Bureau of Reclamation, or its designated agent, shall operate the...

  11. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070...

  12. First report of anther smut caused by Microbotryum violaceum on forked catchfly in Turkey

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Forked catchfly (Silene dichotoma Ehrh.), family Caryophyllaceae, is a common and native plant in rangelands and pastures in Turkey. It is also an introduced plant that is widely distributed in North America. In May, 2007 about 20 forked catchfly plants on the campus of Ondokuz Mayis University, i...

  13. UV stalled replication forks restart by re-priming in human fibroblasts

    PubMed Central

    Elvers, Ingegerd; Johansson, Fredrik; Groth, Petra; Erixon, Klaus; Helleday, Thomas

    2011-01-01

    Restarting stalled replication forks is vital to avoid fatal replication errors. Previously, it was demonstrated that hydroxyurea-stalled replication forks rescue replication either by an active restart mechanism or by new origin firing. To our surprise, using the DNA fibre assay, we only detect a slightly reduced fork speed on a UV-damaged template during the first hour after UV exposure, and no evidence for persistent replication fork arrest. Interestingly, no evidence for persistent UV-induced fork stalling was observed even in translesion synthesis defective, Polηmut cells. In contrast, using an assay to measure DNA molecule elongation at the fork, we observe that continuous DNA elongation is severely blocked by UV irradiation, particularly in UV-damaged Polηmut cells. In conclusion, our data suggest that UV-blocked replication forks restart effectively through re-priming past the lesion, leaving only a small gap opposite the lesion. This allows continuation of replication on damaged DNA. If left unfilled, the gaps may collapse into DNA double-strand breaks that are repaired by a recombination pathway, similar to the fate of replication forks collapsed after hydroxyurea treatment. PMID:21646340

  14. Replication fork movement and methylation govern SeqA binding to the Escherichia coli chromosome.

    PubMed

    Waldminghaus, Torsten; Weigel, Christoph; Skarstad, Kirsten

    2012-07-01

    In Escherichia coli, the SeqA protein binds specifically to GATC sequences which are methylated on the A of the old strand but not on the new strand. Such hemimethylated DNA is produced by progression of the replication forks and lasts until Dam methyltransferase methylates the new strand. It is therefore believed that a region of hemimethylated DNA covered by SeqA follows the replication fork. We show that this is, indeed, the case by using global ChIP on Chip analysis of SeqA in cells synchronized regarding DNA replication. To assess hemimethylation, we developed the first genome-wide method for methylation analysis in bacteria. Since loss of the SeqA protein affects growth rate only during rapid growth when cells contain multiple replication forks, a comparison of rapid and slow growth was performed. In cells with six replication forks per chromosome, the two old forks were found to bind surprisingly little SeqA protein. Cell cycle analysis showed that loss of SeqA from the old forks did not occur at initiation of the new forks, but instead occurs at a time point coinciding with the end of SeqA-dependent origin sequestration. The finding suggests simultaneous origin de-sequestration and loss of SeqA from old replication forks.

  15. Middle Fork and Upper Salmon River Habitat Improvement Implementation Plan, FY 1988-1992.

    SciTech Connect

    Andrews, John; Everson, Larry B.

    1988-02-01

    This document presents an implementation plan for completing the phase II portion of the Middle Fork and Upper Salmon River Habitat Improvement Agreement. Underseeding of spawning adult salmon and steelhead, high instream sediment levels, a lack of habitat diversity in the form of overhanging riparian vegetation and edge, and barriers to both adult and juvenile anadromous fish migration were identified as the principal factors limiting anadromous fish production in the project area. Underseeding is being addressed in other projects sponsored and funded by the Bonneville Power Administration while this implementation plan lays out a schedule for resolving the other identified limiting factors. The primary goal of this program is to increase the quality and quantity of anadromous fish habitat (spring chinook and summer steelhead) with an emphasis on the survival of the wild stocks. This goal will be achieved by reducing the impact of sediment loading, improving riparian vegetation, eliminating passage barriers, and increasing habitat diversity. Meeting the above goal will provide off-site mitigation under the manadate of the pacific northwest electric power planning and conservation act of 1980. Project implementation will follow measures in the Northwest Power Council's Columbia River Fish and Wildlife Program. 9 refs., 3 figs., 5 tabs.

  16. Submicron particles at Thompson Farm during ICARTT measured using aerosol mass spectrometry

    NASA Astrophysics Data System (ADS)

    Cottrell, Laura D.; Griffin, Robert J.; Jimenez, Jose L.; Zhang, Qi; Ulbrich, Ingrid; Ziemba, Luke D.; Beckman, Pieter J.; Sive, Barkley C.; Talbot, Robert W.

    2008-04-01

    The composition and size of aerosols were measured using an Aerodyne quadrupole aerosol mass spectrometer at Thompson Farm in Durham, NH, during the International Consortium for Atmospheric Research on Transport and Transformation campaign during summer 2004. Submicron, non-refractory aerosol was dominated by organic matter and sulfate (averages of 5.7 μg m-3 and 3.6 μg m-3, respectively), with smaller contributions from nitrate and ammonium (averages of 0.3 μg m-3 and 1.02 μg m-3, respectively). Organic aerosol (OA) mass correlates well with anthropogenic tracers such as carbon monoxide (CO, R2 = 0.58) and black carbon (R2 = 0.59), but multiple analyses indicate possible contributions from primary, secondary, anthropogenic, and biogenic OA. Multivariate statistical analysis of the OA mass spectra indicates the presence of two types of oxygenated OA (OOA) and a hydrocarbon-like OA (HOA) component that also contains contributions from biomass burning OA (BBOA). On average, the HOA/BBOA component accounts for 21% of the total OA mass while the two OOA components account for 24% and 55%, respectively, of the OA burden. Observed nitrate correlates well with OA (R2 = 0.67), suggesting interference, the presence of organic nitrates, processing/uptake of nitric acid by OA, or other temporally coincident processes because of the ammonia-poor environment with respect to sulfate. The relative increase of OA with respect to background compared to that of CO (average of 72.7 μg m-3 ppmv-1) indicates values that are higher than those based on previous measurements in New England.

  17. INTEGRATED OUTCROP AND SUBSURFACE STUDIES OF THE INTERWELL ENVIRONMENT OF CARBONATE RESERVOIRS: CLEAR FORK (LEONARDIAN-AGE) RESERVOIRS, WEST TEXAS AND NEW MEXICO

    SciTech Connect

    F. Jerry Lucia

    2002-01-31

    This is the final report of the project ''Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonardian-Age) Reservoirs, West Texas and New Mexico'', Department of Energy contract no. DE-AC26-98BC15105 and is the third in a series of similar projects funded jointly by the U.S. Department of Energy and The University of Texas at Austin, Bureau of Economic Geology, Reservoir Characterization Research Laboratory for Carbonates. All three projects focus on the integration of outcrop and subsurface data for the purpose of developing improved methods for modeling petrophysical properties in the interwell environment. The first project, funded by contract no. DE-AC22-89BC14470, was a study of San Andres outcrops in the Algerita Escarpment, Guadalupe Mountains, Texas and New Mexico, and the Seminole San Andres reservoir, Permian Basin. This study established the basic concepts for constructing a reservoir model using sequence-stratigraphic principles and rock-fabric, petrophysical relationships. The second project, funded by contract no. DE-AC22-93BC14895, was a study of Grayburg outcrops in the Brokeoff Mountains, New Mexico, and the South Cowden Grayburg reservoir, Permian Basin. This study developed a sequence-stratigraphic succession for the Grayburg and improved methods for locating remaining hydrocarbons in carbonate ramp reservoirs. The current study is of the Clear Fork Group in Apache Canyon, Sierra Diablo Mountains, West Texas, and the South Wasson Clear Fork reservoir, Permian Basin. The focus was on scales of heterogeneity, imaging high- and low-permeability layers, and the impact of fractures on reservoir performance. In this study (1) the Clear Fork cycle stratigraphy is defined, (2) important scales of petrophysical variability are confirmed, (3) a unique rock-fabric, petrophysical relationship is defined, (4) a porosity method for correlating high-frequency cycles and defining rock-fabric flow layers

  18. FANCJ couples replication past natural fork barriers with maintenance of chromatin structure

    PubMed Central

    Schwab, Rebekka A.; Nieminuszczy, Jadwiga; Shin-ya, Kazuo

    2013-01-01

    Defective DNA repair causes Fanconi anemia (FA), a rare childhood cancer–predisposing syndrome. At least 15 genes are known to be mutated in FA; however, their role in DNA repair remains unclear. Here, we show that the FANCJ helicase promotes DNA replication in trans by counteracting fork stalling on replication barriers, such as G4 quadruplex structures. Accordingly, stabilization of G4 quadruplexes in ΔFANCJ cells restricts fork movements, uncouples leading- and lagging-strand synthesis and generates small single-stranded DNA gaps behind the fork. Unexpectedly, we also discovered that FANCJ suppresses heterochromatin spreading by coupling fork movement through replication barriers with maintenance of chromatin structure. We propose that FANCJ plays an essential role in counteracting chromatin compaction associated with unscheduled replication fork stalling and restart, and suppresses tumorigenesis, at least partially, in this replication-specific manner. PMID:23530069

  19. Active Control of Repetitive Structural Transitions between Replication Forks and Holliday Junctions by Werner Syndrome Helicase.

    PubMed

    Shin, Soochul; Lee, Jinwoo; Yoo, Sangwoon; Kulikowicz, Tomasz; Bohr, Vilhelm A; Ahn, Byungchan; Hohng, Sungchul

    2016-08-01

    The reactivation of stalled DNA replication via fork regression invokes Holliday junction formation, branch migration, and the recovery of the replication fork after DNA repair or error-free DNA synthesis. The coordination mechanism for these DNA structural transitions by molecular motors, however, remains unclear. Here we perform single-molecule fluorescence experiments with Werner syndrome protein (WRN) and model replication forks. The Holliday junction is readily formed once the lagging arm is unwound, and migrated unidirectionally with 3.2 ± 0.03 bases/s velocity. The recovery of the replication fork was controlled by branch migration reversal of WRN, resulting in repetitive fork regression. The Holliday junction formation, branch migration, and migration direction reversal are all ATP dependent, revealing that WRN uses the energy of ATP hydrolysis to actively coordinate the structural transitions of DNA.

  20. Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.

    PubMed

    Sabatinos, Sarah A; Green, Marc D; Forsburg, Susan L

    2012-12-01

    Hydroxyurea (HU) treatment activates the intra-S phase checkpoint proteins Cds1 and Mrc1 to prevent replication fork collapse. We found that prolonged DNA synthesis occurs in cds1Δ and mrc1Δ checkpoint mutants in the presence of HU and continues after release. This is coincident with increased DNA damage measured by phosphorylated histone H2A in whole cells during release. High-resolution live-cell imaging shows that mutants first accumulate extensive replication protein A (RPA) foci, followed by increased Rad52. Both DNA synthesis and RPA accumulation require the MCM helicase. We propose that a replication fork "collapse point" in HU-treated cells describes the point at which accumulated DNA damage and instability at individual forks prevent further replication. After this point, cds1Δ and mrc1Δ forks cannot complete genome replication. These observations establish replication fork collapse as a dynamic process that continues after release from HU block.

  1. 75 FR 7266 - Roaring Fork Valley Physicians I.P.A.; Analysis of the Agreement Containing Consent Order to Aid...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-18

    ... Roaring Fork Valley Physicians I.P.A.; Analysis of the Agreement Containing Consent Order to Aid Public.... Comments should refer to ``Roaring Fork Valley, File No. 061 0172'' to facilitate the organization of... release describing it. A comment filed in paper form should include the ``Roaring Fork Valley, File...

  2. 75 FR 41237 - Public Land Order No. 7746; Withdrawal of Public Lands, South Fork of the American River; California

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... Bureau of Land Management Public Land Order No. 7746; Withdrawal of Public Lands, South Fork of the... recreational values along the South Fork of the American River. DATES: Effective Date: July 15, 2010. ADDRESSES... along the South Fork of the American River. Order By virtue of the authority vested in the Secretary...

  3. The stability of nucleosomes at the replication fork.

    PubMed

    Gasser, R; Koller, T; Sogo, J M

    1996-05-01

    Purified simian virus (SV40) minichromosomes were photoreacted with psoralen under various conditions that moderately destabilize nucleosomes. This assay allows indirect distinction between stable nucleosomes, partially unravelled nucleosomes and nucleosomes containing (or lacking) histone H1. In replicating molecules the passage of the replication machinery destabilizes the nucleosomal organization of the chromatin fiber over a distance of 650 to 1100 bp. In front of the fork, an average of two nucleosomes are destabilized presumably by the dissociation of histone H1 and the advancing replication machinery. On daughter strands, the first nucleosome is detected at a distance of about 260 nucleotides from the elongation point. This nucleosome is interpreted to contain no histone H1, while no stepwise association of (H3-H4)2 tetramers with H2A/H2B dimers on nascent DNA can be detected in vivo. The second nucleosome after the replication fork appears to contain histone H1. The prolonged nuclease sensitivity of newly replicated chromatin described in the literature therefore may not be due to a slow reassociation of histone H1.

  4. Environment of deposition of Clear Fork Formation: Yoakum County, Texas

    SciTech Connect

    Moore, B.K.

    1987-05-01

    The Clear Fork Formation is Permian (Leonardian) in age and constitutes a major oil-bearing unit in the Permian basin of west Texas. In Yoakum County, west Texas, the upper Clear Fork carbonates record a subtidal upward-shoaling sequence of deposition. A small bryozoan-algal patch reef is situated within these carbonates near the southern edge of the North Basin platform. The reef is completely dolomitized, but paramorphic replacement has facilitated a study of the paleoecology, lateral variations, and community succession within this buildup. Build-ups of this type are scarcely known in strata of Permian age. The reef was apparently founded on a coquina horizon at the base of the buildup. The reef apparently had a low-relief, dome-shaped morphology. The trapping and binding of sediment by bryozoa appear to have been the main constructional process. A significant role was also played by encrusting forams and the early precipitation of submarine cements, both of which added rigidity to the structure. The reef also contains a low-diversity community of other invertebrates. Algal constituents predominate at the basinward edge of the buildup. The reef was formed entirely subaqueously on a broad, relatively shallow tropical marine carbonate shelf environment. An understanding of the lithofacies distribution and paragenesis within this sequence will provide information on porosity variations and the nature and distribution of permeability barriers. Such information is useful in reservoir modeling studies and for secondary recovery techniques in shelf-edge carbonate reservoirs of this type.

  5. BRCA1 controls homologous recombination at Tus/Ter-stalled mammalian replication forks

    PubMed Central

    Willis, Nicholas A.; Chandramouly, Gurushankar; Huang, Bin; Kwok, Amy; Follonier, Cindy; Deng, Chuxia; Scully, Ralph

    2014-01-01

    Replication fork stalling can promote genomic instability, predisposing to cancer and other diseases1–3. Stalled replication forks may be processed by sister chromatid recombination (SCR), generating error-free or error-prone homologous recombination (HR) outcomes4–8. In mammalian cells, a long-standing hypothesis proposes that the major hereditary breast/ovarian cancer predisposition gene products, BRCA1 and BRCA2, control HR/SCR at stalled replication forks9. Although BRCA1 and BRCA2 affect replication fork processing10–12, direct evidence that BRCA genes regulate HR at stalled chromosomal replication forks is lacking due to a dearth of tools for studying this process. We report that the Escherichia coli Tus/Ter complex13–16 can be engineered to induce site-specific replication fork stalling and chromosomal HR/SCR in mammalian cells. Tus/Ter-induced HR entails processing of bidirectionally arrested forks. We find that the BRCA1 C-terminal tandem BRCT repeat and regions of BRCA1 encoded by exon 11—two BRCA1 elements implicated in tumor suppression—control Tus/Ter-induced HR. Inactivation of either BRCA1 or BRCA2 increases the absolute frequency of “long-tract” gene conversions at Tus/Ter-stalled forks—an outcome not observed in response to a restriction endonuclease-mediated chromosomal double strand break (DSB). Therefore, HR at stalled forks is regulated differently from HR at DSBs arising independently of a fork. We propose that aberrant long-tract HR at stalled replication forks contributes to genomic instability and breast/ovarian cancer predisposition in BRCA mutant cells. PMID:24776801

  6. South Fork Flathead Watershed Westslope Cutthroat Trout Conservation Program, Annual Report 2002.

    SciTech Connect

    Grisak, Grant; Marotz, Brian

    2003-06-01

    In 1999, Montana Fish, Wildlife & Parks (MFWP) began a program aimed at conserving the genetically pure populations of westslope cutthroat trout in the South Fork Flathead River drainage. The objective of this program is to eliminate all of the exotic and hybrid trout that threaten the genetically pure westslope cutthroat populations in the South Fork Flathead. The exotic and hybrid trout populations occur in several headwater lakes and their outflow streams. In 2001 MFWP released a draft environmental assessment, pursuant to the Montana Environmental Policy Act (MEPA), that addressed the use of motorized equipment to deliver personnel and materials to some of these lakes in the Bob Marshall and Great Bear Wildernesses (Grisak 2001). After a 30-day public comment period, MFWP determined that the complexity of issues was too great and warranted a more detailed analysis. These issues included transportation options for personnel, equipment and materials, the use of motorized equipment in wilderness, fish removal methods, fish stocking, and the status and distribution of amphibian populations in the project area. Because the program also involves the U.S. Forest Service (USFS) and Bonneville Power Administration (BPA), the environmental analysis needs to comply with the National Environmental Policy Act (NEPA). In October 2001, pursuant to NEPA, MFWP, along with the USFS and BPA initiated an environmental assessment to address these issues. In June 2002, the three agencies determined that the scope of these issues warranted an Environmental Impact Statement. This specialist report describes the logistical, technical and biological issues associated with this project and provides an analysis of options for fish removal, transportation and fish stocking. It further analyzes issues and concerns associated with amphibian populations and creating new domesticated stocks of westslope cutthroat trout. Finally, this document provides a description of each lake, the best

  7. Supplement Analysis for the Watershed Management Program EIS (DOE/EIS-0265/SA-109) - East Fork Holistic Restoration – Salmon River East Fork (SEF) 12 and Herd Creek (HC) 1

    SciTech Connect

    Stewart, Shannon C.

    2003-07-22

    The Bonneville Power Administration (BPA) is proposing to fund activities that will improve fish passage at Salmon River East Fork diversion 12 and at Herd Creek diversion 1. These projects represent cooperative efforts between the Custer Soil and Water Conservation District, Idaho Department of Fish and Game, and local irrigators. The goal of the SEF 12 project is to improve fish passage and habitat by removing the existing SEF 12 pushup gravel diversion, replacing it with a permanent rock weir structure, and installing an impervious membrane and geotextile liner in the wing diversion parallel to the stream bank. The work on the diversion structure at HC 1 will be confined to the existing headgate, wasteway and plunge pool immediately below the structure. The wasteway and plunge pool are part of the system that provides fish passage around the existing diversion structure. The new structure will include improved stop logs and a reconstructed plunge pool, which will enhance fish passage at the diversion.

  8. Oxbow Conservation Area; Middle Fork John Day River, Annual Report 2003-2004.

    SciTech Connect

    Cochran, Brian

    2004-02-01

    In early 2001, the Confederated Tribes of Warm Springs, through their John Day Basin Office, concluded the acquisition of the Oxbow Ranch, now know as the Oxbow Conservation Area (OCA). Under a memorandum of agreement with the Bonneville Power Administration (BPA), the Tribes are required to provided BPA an 'annual written report generally describing the real property interests in the Project, HEP analyses undertaken or in progress, and management activities undertaken or in progress'. The project during 2003 was crippled due to the aftermath of the BPA budget crisis. Some objectives were not completed during the first half of this contract because of limited funds in the 2003 fiscal year. The success of this property purchase can be seen on a daily basis. Water rights were utilized only in the early, high water season and only from diversion points with functional fish screens. After July 1, all of the OCA water rights were put instream. Riparian fences on the river, Ruby and Granite Boulder creeks continued to promote important vegetation to provide shade and bank stabilization. Hundreds of willow, dogwood, Douglas-fir, and cottonwood were planted along the Middle Fork John Day River. Livestock grazing on the property was carefully managed to ensure the protection of fish and wildlife habitat, while promoting meadow vigor and producing revenue for property taxes. Monitoring of property populations, resources, and management activities continued in 2003 to build a database for future management of this and other properties in the region.

  9. Apollo Project

    NASA Technical Reports Server (NTRS)

    1966-01-01

    From Spaceflight Revolution: 'Top NASA officials listen to a LOPO briefing at Langley in December 1966. Sitting to the far right with his hand on his chin is Floyd Thompson. To the left sits Dr. George Mueller, NASA associate administrator for Manned Space Flight. On the wall is a diagram of the sites selected for the 'concentrated mission.' 'The most fundamental issue in the pre-mission planning for Lunar Orbiter was how the moon was to be photographed. Would the photography be 'concentrated' on a predetermined single target, or would it be 'distributed' over several selected targets across the moon's surface? On the answer to this basic question depended the successful integration of the entire mission plan for Lunar Orbiter.' The Lunar Orbiter Project made systematic photographic maps of the lunar landing sites. Published in James R. Hansen, Spaceflight Revolution: NASA Langley Research Center From Sputnik to Apollo, (Washington: NASA, 1995), p. 337.

  10. Horvitz-Thompson survey sample methods for estimating large-scale animal abundance

    USGS Publications Warehouse

    Samuel, M.D.; Garton, E.O.

    1994-01-01

    Large-scale surveys to estimate animal abundance can be useful for monitoring population status and trends, for measuring responses to management or environmental alterations, and for testing ecological hypotheses about abundance. However, large-scale surveys may be expensive and logistically complex. To ensure resources are not wasted on unattainable targets, the goals and uses of each survey should be specified carefully and alternative methods for addressing these objectives always should be considered. During survey design, the impoflance of each survey error component (spatial design, propofiion of detected animals, precision in detection) should be considered carefully to produce a complete statistically based survey. Failure to address these three survey components may produce population estimates that are inaccurate (biased low), have unrealistic precision (too precise) and do not satisfactorily meet the survey objectives. Optimum survey design requires trade-offs in these sources of error relative to the costs of sampling plots and detecting animals on plots, considerations that are specific to the spatial logistics and survey methods. The Horvitz-Thompson estimators provide a comprehensive framework for considering all three survey components during the design and analysis of large-scale wildlife surveys. Problems of spatial and temporal (especially survey to survey) heterogeneity in detection probabilities have received little consideration, but failure to account for heterogeneity produces biased population estimates. The goal of producing unbiased population estimates is in conflict with the increased variation from heterogeneous detection in the population estimate. One solution to this conflict is to use an MSE-based approach to achieve a balance between bias reduction and increased variation. Further research is needed to develop methods that address spatial heterogeneity in detection, evaluate the effects of temporal heterogeneity on survey

  11. Surface Ozone Differences Between Appledore Island and Thompson Farm: Local-Scale vs. Synoptic Scale Meteorology

    NASA Astrophysics Data System (ADS)

    Darby, L. S.; Senff, C. J.

    2003-12-01

    At NEAQS 2002, the surface O3 observation network included stations at Thompson Farm (TF), near Durham, NH, and Appledore Island (AI), just off the coast of NH. The stations were only ~30 km apart, yet the differences in the O3 measured at the two sites could be as high as 50 ppbv. This study focuses on meteorological processes contributing to the differences in O3 at the two sites. During the first 6 days of Aug. 2002, the differences in surface O3 measured at AI and TF ranged from < 5 ppbv to > than 50 ppbv, with AI typically having higher O3 than TF. Meteorology played a role in these differences. For instance, on 6 Aug., a day when the ozone differences between the two sites were very small throughout the day (< 10 ppbv), there were no local-scale circulations such as a sea breeze, and both TF and AI were in the same post-frontal northwesterly, `clean' (low-O3) air. In contrast, on two of the days with large differences in surface ozone between the stations, there was a synoptic stationary front hugging the New England coast, separating the marine air mass (sampled at AI) from the continental air mass (sampled at TF). Differences in large-scale ozone transport within these two air masses led to much higher ozone values at AI than at TF, with differences as large as 50 ppbv. The formation and inland propagation of a sea breeze, measured by the ETL Doppler lidar, acted to lessen the effect of the stationary front on a local scale, bringing higher-ozone air to TF, reducing the difference in ozone between the two sites. Other features receiving further investigation will include the Appalachian Trough and the few times during which TF ozone measurements exceeded those at AI. The Appalachian Trough enhances the southwest transport of pollutants from the New York and Boston areas to the NH and ME coasts. This feature was in place from late in the day on 4 Aug. and continued through 5 Aug. During this period, ozone measurements at both sites were elevated relative to

  12. Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork.

    PubMed

    Calzada, Arturo; Hodgson, Ben; Kanemaki, Masato; Bueno, Avelino; Labib, Karim

    2005-08-15

    Eukaryotic cells regulate the progression and integrity of DNA replication forks to maintain genomic stability and couple DNA synthesis to other processes. The budding yeast proteins Mrc1 and Tof1 associate with the putative MCM-Cdc45 helicase and limit progression of the replisome when nucleotides are depleted, and the checkpoint kinases Mec1 and Rad53 stabilize such stalled forks and prevent disassembly of the replisome. Forks also pause transiently during unperturbed chromosome replication, at sites where nonnucleosomal proteins bind DNA tightly. We describe a method for inducing prolonged pausing of forks at protein barriers assembled at unique sites on a yeast chromosome, allowing us to examine for the first time the effects of pausing upon replisome integrity. We show that paused forks maintain an intact replisome that contains Mrc1, Tof1, MCM-Cdc45, GINS, and DNA polymerases alpha and epsilon and that recruits the Rrm3 helicase. Surprisingly, pausing does not require Mrc1, although Tof1 and Csm3 are both important. In addition, the integrity of the paused forks does not require Mec1, Rad53, or recombination. We also show that paused forks at analogous barriers in the rDNA are regulated similarly. These data indicate that paused and stalled eukaryotic replisomes resemble each other but are regulated differently.

  13. Replication forks reverse at high frequency upon replication stress in Physarum polycephalum.

    PubMed

    Maric, Chrystelle; Bénard, Marianne

    2014-12-01

    The addition of hydroxyurea after the onset of S phase allows replication to start and permits the successive detecting of replication-dependent joint DNA molecules and chicken foot structures in the synchronous nuclei of Physarum polycephalum. We find evidence for a very high frequency of reversed replication forks upon replication stress. The formation of these reversed forks is dependent on the presence of joint DNA molecules, the impediment of the replication fork progression by hydroxyurea, and likely on the propensity of some replication origins to reinitiate replication to counteract the action of this compound. As hydroxyurea treatment enables us to successively detect the appearance of joint DNA molecules and then of reversed replication forks, we propose that chicken foot structures are formed both from the regression of hydroxyurea-frozen joint DNA molecules and from hydroxyurea-stalled replication forks. These experiments underscore the transient nature of replication fork regression, which becomes detectable due to the hydroxyurea-induced slowing down of replication fork progression.

  14. Geomorphic Characterization of the Middle Fork Saline River: Garland, Perry, and Saline Counties, Arkansas

    USGS Publications Warehouse

    Pugh, Aaron L.; Garday, Thomas J.; Redman, Ronald

    2008-01-01

    This report was prepared to help address concerns raised by local residents, State, and Federal agencies about the current geomorphic conditions of the Middle Fork Saline River. Over the past 30 years the Middle Fork Saline River Basin has experienced a marked increase in urbanization. The report summarizes the Middle Fork?s current (2003) channel characteristics at nine stream reaches in the upper 91 square miles of the basin. Assessments at each study reach included comparing measured stream geometry dimensions (cross-sectional area, top width, and mean depth) at bankfull stage to regional hydraulic geometry curves for the Ouachita Mountains Physiographic Province of Arkansas and Oklahoma, evaluations of streambed materials and sinuosity, and classification of individual stream reach types. When compared to the Ouachita Mountains? regional hydraulic geometry curves for natural, stable, stream reaches, five of the nine study reaches had slightly smaller crosssectional areas, longer top widths, and shallower depths. Streambed material analysis indicates that the Middle Fork is a bedrock influenced, gravel dominated stream with lesser amounts of sand and cobbles. Slight increases in sinuosity from 1992 to 2002 at seven of the nine study reaches indicate a slight decrease in stream channel slope. Analyses of the Middle Fork?s hydraulic geometry and sinuosity indicate that the Middle Fork is currently overly wide and shallow, but is slowly adjusting towards a deeper, narrower hydraulic geometry. Using the Rosgen system of channel classification, the two upstream study reaches classified as B4c/1 stream types; which were moderately entrenched, riffle dominated channels, with infrequently spaced pools. The downstream seven study reaches classified as C4/1 stream types; which were slightly entrenched, meandering, gravel-dominated, riffle/ pool channels with well developed flood plains. Analyses of stream reach types suggest that the downstream reaches of the Middle Fork

  15. Effects of Transposable Elements on the Expression of the Forked Gene of Drosophila Melanogaster

    PubMed Central

    Hoover, K. K.; Chien, A. J.; Corces, V. G.

    1993-01-01

    The products of the forked gene are involved in the formation and/or maintenance of a temporary fibrillar structure within the developing bristle rudiment of Drosophila melanogaster. Mutations in the forked locus alter this structure and result in aberrant development of macrochaetae, microchaetae and trichomes. The locus has been characterized at the molecular level by walking, mutant characterization and transcript analysis. Expression of the six forked transcripts is temporally restricted to midlate pupal development. At this time, RNAs of 6.4, 5.6, 5.4, 2.5, 1.9 and 1.1 kilobases (kb) are detected by Northern analysis. The coding region of these RNAs has been found to be within a 21-kb stretch of genomic DNA. The amino terminus of the proteins encoded by the 5.4- and 5.6-kb forked transcripts contain tandem copies of ankyrin-like repeats that may play an important role in the function of forked-encoded products. The profile of forked RNA expression is altered in seven spontaneous mutations characterized during this study. Three forked mutations induced by the insertion of the gypsy retrotransposon contain a copy of this element inserted into an intron of the gene. In these mutants, the 5.6-, 5.4- and 2.5-kb forked mRNAs are truncated via recognition of the polyadenylation site in the 5' long terminal repeat of the gypsy retrotransposon. These results help explain the role of the forked gene in fly development and further our understanding of the role of transposable elements in mutagenesis. PMID:8244011

  16. Improved Tuning Fork for Terahertz Quartz-Enhanced Photoacoustic Spectroscopy

    PubMed Central

    Sampaolo, Angelo; Patimisco, Pietro; Giglio, Marilena; Vitiello, Miriam S.; Beere, Harvey E.; Ritchie, David A.; Scamarcio, Gaetano; Tittel, Frank K.; Spagnolo, Vincenzo

    2016-01-01

    We report on a quartz-enhanced photoacoustic (QEPAS) sensor for methanol (CH3OH) detection employing a novel quartz tuning fork (QTF), specifically designed to enhance the QEPAS sensing performance in the terahertz (THz) spectral range. A discussion of the QTF properties in terms of resonance frequency, quality factor and acousto-electric transduction efficiency as a function of prong sizes and spacing between the QTF prongs is presented. The QTF was employed in a QEPAS sensor system using a 3.93 THz quantum cascade laser as the excitation source in resonance with a CH3OH rotational absorption line located at 131.054 cm−1. A minimum detection limit of 160 ppb in 30 s integration time, corresponding to a normalized noise equivalent absorption NNEA = 3.75 × 10−11 cm−1W/Hz½, was achieved, representing a nearly one-order-of-magnitude improvement with respect to previous reports. PMID:27023552

  17. Wavelike movement of bedload sediment, East Fork River, Wyoming

    USGS Publications Warehouse

    Meade, R.H.

    1985-01-01

    Bedload is moved down the East Fork River in distinct wavelike pulses that have the form of composite dune fields The moving material consists mostly of coarse sand and fine gravel The wavelengths of the pulses are about 500-600 m, a distance that is predetermined by the pattern of stoage of bed sediment in the river during low water As the river discharge increases, the bed sediment is scoured from the storage areas, and it is moved onto and across the interventing riffles As the river discharge decreases, the bed sediment is scoured off the riffles and moved into the next storage area downstream Each successive pulse of water discharge sets into motion a wave of bedload that continues to move unitil it reaches the next storage area ?? 1985 Springer-Verlag New York Inc.

  18. Quartz-enhanced photoacoustic spectroscopy exploiting tuning fork overtone modes

    NASA Astrophysics Data System (ADS)

    Sampaolo, A.; Patimisco, P.; Dong, L.; Geras, A.; Scamarcio, G.; Starecki, T.; Tittel, F. K.; Spagnolo, V.

    2015-12-01

    We report on a quartz-enhanced photoacoustic sensor (QEPAS) based on a custom-made quartz tuning fork (QTF) to operate in both the fundamental and the first overtone vibrational mode resonances. The QTF fundamental mode resonance falls at ˜3 kHz and the first overtone at ˜18 kHz. Electrical tests showed that the first overtone provides a higher quality factor and increased piezoelectric current peak values, with respect to the fundamental flexural mode. To evaluate the QTF acousto-electric energy conversion efficiency, we operated the QEPAS in the near-IR and selected water vapor as the target gas. The first overtone resonance provides a QEPAS signal-to-noise ratio ˜5 times greater with respect to that measured for the fundamental mode. These results open the way to employing QTF overtone vibrational modes for QEPAS based trace gas sensing.

  19. Finite element analysis of electrically excited quartz tuning fork devices.

    PubMed

    Oria, Roger; Otero, Jorge; González, Laura; Botaya, Luis; Carmona, Manuel; Puig-Vidal, Manel

    2013-05-30

    Quartz Tuning Fork (QTF)-based Scanning Probe Microscopy (SPM) is an important field of research. A suitable model for the QTF is important to obtain quantitative measurements with these devices. Analytical models have the limitation of being based on the double cantilever configuration. In this paper, we present an electromechanical finite element model of the QTF electrically excited with two free prongs. The model goes beyond the state-of-the-art of numerical simulations currently found in the literature for this QTF configuration. We present the first numerical analysis of both the electrical and mechanical behavior of QTF devices. Experimental measurements obtained with 10 units of the same model of QTF validate the finite element model with a good agreement.

  20. Quartz tuning forks with novel geometries for optoacoustic gas sensing

    NASA Astrophysics Data System (ADS)

    Spagnolo, V.; Sampaolo, A.; Patimisco, P.; Dong, L.; Gupta, Y.; Yu, Y.; Geras, A.; Giglio, M.; Calabrese, P. P.; Starecki, T.; Scamarcio, G.; Tittel, Frank K.

    2016-02-01

    We report the successful realization of quartz-enhanced photo-acoustic (QEPAS) sensors employing quartz tuning forks (QTFs) with novel geometrical parameters. We investigated the influence of QTF sizes on the main resonator parameters, in order to identify the best design parameters optimizing the QTF figures of merit for optoacoustic gas sensing. To evaluate the QTF acousto-electric energy conversion efficiency, we operated the QEPAS sensors in the near- IR and selected water vapor as the target gas. QTFs are forced to resonate at both the fundamental and the first overtone vibrational mode frequencies. Our results shows that two QTF designs exhibit an higher quality factor (and consequently an higher QEPAS signal) when operating on the first overtone mode with respect to the fundamental one.

  1. Mechanism of asymmetric polymerase assembly at the eukaryotic replication fork

    PubMed Central

    Georgescu, Roxana E; Langston, Lance; Yao, Nina Y; Yurieva, Olga; Zhang, Dan; Finkelstein, Jeff; Agarwal, Tani; O’Donnell, Mike E

    2015-01-01

    Eukaryotes use distinct polymerases for leading- and lagging-strand replication, but how they target their respective strands is uncertain. We reconstituted Saccharomyces cerevisiae replication forks and found that CMG helicase selects polymerase (Pol) ε to the exclusion of Pol δ on the leading strand. Even if Pol δ assembles on the leading strand, Pol ε rapidly replaces it. Pol δ–PCNA is distributive with CMG, in contrast to its high stability on primed ssDNA. Hence CMG will not stabilize Pol δ, instead leaving the leading strand accessible for Pol ε and stabilizing Pol ε. Comparison of Pol ε and Pol δ on a lagging-strand model DNA reveals the opposite. Pol δ dominates over excess Pol ε on PCNA-primed ssDNA. Thus, PCNA strongly favors Pol δ over Pol ε on the lagging strand, but CMG over-rides and flips this balance in favor of Pol ε on the leading strand. PMID:24997598

  2. Mechanism of asymmetric polymerase assembly at the eukaryotic replication fork.

    PubMed

    Georgescu, Roxana E; Langston, Lance; Yao, Nina Y; Yurieva, Olga; Zhang, Dan; Finkelstein, Jeff; Agarwal, Tani; O'Donnell, Mike E

    2014-08-01

    Eukaryotes use distinct polymerases for leading- and lagging-strand replication, but how they target their respective strands is uncertain. We reconstituted Saccharomyces cerevisiae replication forks and found that CMG helicase selects polymerase (Pol) ɛ to the exclusion of Pol δ on the leading strand. Even if Pol δ assembles on the leading strand, Pol ɛ rapidly replaces it. Pol δ-PCNA is distributive with CMG, in contrast to its high stability on primed ssDNA. Hence CMG will not stabilize Pol δ, instead leaving the leading strand accessible for Pol ɛ and stabilizing Pol ɛ. Comparison of Pol ɛ and Pol δ on a lagging-strand model DNA reveals the opposite. Pol δ dominates over excess Pol ɛ on PCNA-primed ssDNA. Thus, PCNA strongly favors Pol δ over Pol ɛ on the lagging strand, but CMG over-rides and flips this balance in favor of Pol ɛ on the leading strand. PMID:24997598

  3. MFH-1, a new member of the fork head domain family, is expressed in developing mesenchyme.

    PubMed

    Miura, N; Wanaka, A; Tohyama, M; Tanaka, K

    1993-07-12

    We have isolated a novel mouse gene, MFH-1 (mesenchyme fork head 1) that is related to the Drosophila fork head and rat HNF3 genes. MFH-1 encodes a distinct fork head domain that is classified into a distinct subfamily. A recombinant MFH-1 protein could bind to the HNF3 binding site. MFH-1 is expressed temporally in developing embryos, first in the non-notochordal mesoderm and later in areas of mesenchymal condensation in the trunk, head, and limbs. Our results suggest that MFH-1 might be involved in the formation of special mesenchymal tissues.

  4. Geochemical map of the North Fork John Day River Roadless Area, Grant County, Oregon

    USGS Publications Warehouse

    Evans, James G.

    1986-01-01

    The North Fork John Day River Roadless Area comprised 21,210 acres in the Umatilla and Wallowa-Whitman National Forests, Grant County, Oregon, about 30 miles northwest of Baker, Oregon. The irregularly shaped area extends for about 1 mile on both sides of a 25-mile segment of the North Fork John Day River from Big Creek on the west to North Fork John Day Campground on the east. Most of the roadless area is in the northern half of the Desolation Butte 15-minute quadrangle. The eastern end of the area is in parts of the Granite and Trout Meadows 7½-minute quadrangles.

  5. Crossover from hydrodynamic to acoustic drag on quartz tuning forks in normal and superfluid 4He

    NASA Astrophysics Data System (ADS)

    Bradley, D. I.; Človečko, M.; Fisher, S. N.; Garg, D.; Guise, E.; Haley, R. P.; Kolosov, O.; Pickett, G. R.; Tsepelin, V.; Schmoranzer, D.; Skrbek, L.

    2012-01-01

    We present measurements of the drag forces on quartz tuning forks oscillating at low velocities in normal and superfluid 4He. We have investigated the dissipative drag over a wide range of frequencies, from 6.5 to 600 kHz, by using arrays of forks with varying prong lengths and by exciting the forks in their fundamental and first overtone modes. At low frequencies the behavior is dominated by laminar hydrodynamic drag, governed by the fluid viscosity. At higher frequencies acoustic drag is dominant and is described well by a three-dimensional model of sound emission.

  6. ATR-like kinase Mec1 facilitates both chromatin accessibility at DNA replication forks and replication fork progression during replication stress.

    PubMed

    Rodriguez, Jairo; Tsukiyama, Toshio

    2013-01-01

    Faithful DNA replication is essential for normal cell division and differentiation. In eukaryotic cells, DNA replication takes place on chromatin. This poses the critical question as to how DNA replication can progress through chromatin, which is inhibitory to all DNA-dependent processes. Here, we developed a novel genome-wide method to measure chromatin accessibility to micrococcal nuclease (MNase) that is normalized for nucleosome density, the NCAM (normalized chromatin accessibility to MNase) assay. This method enabled us to discover that chromatin accessibility increases specifically at and ahead of DNA replication forks in normal S phase and during replication stress. We further found that Mec1, a key regulatory ATR-like kinase in the S-phase checkpoint, is required for both normal chromatin accessibility around replication forks and replication fork rate during replication stress, revealing novel functions for the kinase in replication stress response. These results suggest a possibility that Mec1 may facilitate DNA replication fork progression during replication stress by increasing chromatin accessibility around replication forks.

  7. Petrology and depositional environments of lower Tuscaloosa Formation (upper Cretaceous) sandstones in North Hustler and Thompson field areas, southwestern Mississippi

    SciTech Connect

    Klicman, D.P.; Cameron, C.P.; Meylan, M.A.

    1988-09-01

    The lithology, diagenesis, and depositional environments of the Dykes, Denkman, and McComb units of the Stringer Sand Member of the lower Tuscaloosa Formation in the North Hustler and Thompson field areas of Amite County, Mississippi, have been determined by detailed description of cores from nine wells and examination of 61 thin sections. The cores were recovered from driller depths of about 11,100 ft (3383 m) in the updip Tuscaloosa trend, which is being actively explored for stratigraphically trapped oil. Most of the sandstone samples (65%) are quartzarenites, and the remainder are sublitharenites. Porosity is mostly secondary, resulting from partial dissolution of framework grains and cement. The depositional environment of the Dykes and Denkman sandstones in all wells is interpreted to be fluvial, with stacked point bars obvious in one well. The McComb sandstone, best developed in a wildcat well between Thompson and McComb fields, is marine, based on the occurrence of glauconite and shell fragments. The presence of a basal scour surface on shale indicates that this sandstone may be the product of marine reworking of a transgressed fluvial (distributary point or mouth bar.)sand.

  8. Stalled DNA Replication Forks at the Endogenous GAA Repeats Drive Repeat Expansion in Friedreich's Ataxia Cells.

    PubMed

    Gerhardt, Jeannine; Bhalla, Angela D; Butler, Jill Sergesketter; Puckett, James W; Dervan, Peter B; Rosenwaks, Zev; Napierala, Marek

    2016-08-01

    Friedreich's ataxia (FRDA) is caused by the expansion of GAA repeats located in the Frataxin (FXN) gene. The GAA repeats continue to expand in FRDA patients, aggravating symptoms and contributing to disease progression. The mechanism leading to repeat expansion and decreased FXN transcription remains unclear. Using single-molecule analysis of replicated DNA, we detected that expanded GAA repeats present a substantial obstacle for the replication machinery at the FXN locus in FRDA cells. Furthermore, aberrant origin activation and lack of a proper stress response to rescue the stalled forks in FRDA cells cause an increase in 3'-5' progressing forks, which could enhance repeat expansion and hinder FXN transcription by head-on collision with RNA polymerases. Treatment of FRDA cells with GAA-specific polyamides rescues DNA replication fork stalling and alleviates expansion of the GAA repeats, implicating DNA triplexes as a replication impediment and suggesting that fork stalling might be a therapeutic target for FRDA.

  9. Topoisomerase I poisoning results in PARP-mediated replication fork reversal.

    PubMed

    Ray Chaudhuri, Arnab; Hashimoto, Yoshitami; Herrador, Raquel; Neelsen, Kai J; Fachinetti, Daniele; Bermejo, Rodrigo; Cocito, Andrea; Costanzo, Vincenzo; Lopes, Massimo

    2012-04-01

    Topoisomerase I (Top1) releases torsional stress during DNA replication and transcription and is inhibited by camptothecin and camptothecin-derived cancer chemotherapeutics. Top1 inhibitor cytotoxicity is frequently linked to double-strand break (DSB) formation as a result of Top1 being trapped on a nicked DNA intermediate in replicating cells. Here we use yeast, mammalian cell lines and Xenopus laevis egg extracts to show that Top1 poisons rapidly induce replication-fork slowing and reversal, which can be uncoupled from DSB formation at sublethal inhibitor doses. Poly(ADP-ribose) polymerase activity, but not single-stranded break repair in general, is required for effective fork reversal and limits DSB formation. These data identify fork reversal as a means to prevent chromosome breakage upon exogenous replication stress and implicate proteins involved in fork reversal or restart as factors modulating the cytotoxicity of replication stress-inducing chemotherapeutics. PMID:22388737

  10. DNA damage-induced replication fork regression and processing in Escherichia coli.

    PubMed

    Courcelle, Justin; Donaldson, Janet R; Chow, Kin-Hoe; Courcelle, Charmain T

    2003-02-14

    DNA lesions that block replication are a primary cause of rearrangements, mutations, and lethality in all cells. After ultraviolet (UV)-induced DNA damage in Escherichia coli, replication recovery requires RecA and several other recF pathway proteins. To characterize the mechanism by which lesion-blocked replication forks recover, we used two-dimensional agarose gel electrophoresis to show that replication-blocking DNA lesions induce a transient reversal of the replication fork in vivo. The reversed replication fork intermediate is stabilized by RecA and RecF and is degraded by the RecQ-RecJ helicase-nuclease when these proteins are absent. We propose that fork regression allows repair enzymes to gain access to the replication-blocking lesion, allowing processive replication to resume once the blocking lesion is removed. PMID:12543983

  11. Theoretical model and optimization of a novel temperature sensor based on quartz tuning fork resonators

    NASA Astrophysics Data System (ADS)

    Jun, Xu; Bo, You; Xin, Li; Juan, Cui

    2007-12-01

    To accurately measure temperatures, a novel temperature sensor based on a quartz tuning fork resonator has been designed. The principle of the quartz tuning fork temperature sensor is that the resonant frequency of the quartz resonator changes with the variation in temperature. This type of tuning fork resonator has been designed with a new doubly rotated cut work at flexural vibration mode as temperature sensor. The characteristics of the temperature sensor were evaluated and the results sufficiently met the target of development for temperature sensor. The theoretical model for temperature sensing has been developed and built. The sensor structure was analysed by finite element method (FEM) and optimized, including tuning fork geometry, tine electrode pattern and the sensor's elements size. The performance curve of output versus measured temperature is given. The results from theoretical analysis and experiments indicate that the sensor's sensitivity can reach 60 ppm °C-1 with the measured temperature range varying from 0 to 100 °C.

  12. Stalled DNA Replication Forks at the Endogenous GAA Repeats Drive Repeat Expansion in Friedreich's Ataxia Cells.

    PubMed

    Gerhardt, Jeannine; Bhalla, Angela D; Butler, Jill Sergesketter; Puckett, James W; Dervan, Peter B; Rosenwaks, Zev; Napierala, Marek

    2016-08-01

    Friedreich's ataxia (FRDA) is caused by the expansion of GAA repeats located in the Frataxin (FXN) gene. The GAA repeats continue to expand in FRDA patients, aggravating symptoms and contributing to disease progression. The mechanism leading to repeat expansion and decreased FXN transcription remains unclear. Using single-molecule analysis of replicated DNA, we detected that expanded GAA repeats present a substantial obstacle for the replication machinery at the FXN locus in FRDA cells. Furthermore, aberrant origin activation and lack of a proper stress response to rescue the stalled forks in FRDA cells cause an increase in 3'-5' progressing forks, which could enhance repeat expansion and hinder FXN transcription by head-on collision with RNA polymerases. Treatment of FRDA cells with GAA-specific polyamides rescues DNA replication fork stalling and alleviates expansion of the GAA repeats, implicating DNA triplexes as a replication impediment and suggesting that fork stalling might be a therapeutic target for FRDA. PMID:27425605

  13. The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability.

    PubMed

    Steinacher, Roland; Osman, Fekret; Dalgaard, Jacob Z; Lorenz, Alexander; Whitby, Matthew C

    2012-03-15

    Bidirectionally moving DNA replication forks merge at termination sites composed of accidental or programmed DNA-protein barriers. If merging fails, then regions of unreplicated DNA can result in the breakage of DNA during mitosis, which in turn can give rise to genome instability. Despite its importance, little is known about the mechanisms that promote the final stages of fork merging in eukaryotes. Here we show that the Pif1 family DNA helicase Pfh1 plays a dual role in promoting replication fork termination. First, it facilitates replication past DNA-protein barriers, and second, it promotes the merging of replication forks. A failure of these processes in Pfh1-deficient cells results in aberrant chromosome segregation and heightened genome instability.

  14. Characterization and Optimization of Quartz Tuning Fork-Based Force Sensors for Combined STM/AFM

    NASA Astrophysics Data System (ADS)

    Castellanos-Gomez, Andres; Agraït, Nicolás; Rubio-Bollinger, Gabino

    This chapter will be divided in two main parts. In the first one, we will show a detailed analysis of the dynamics of quartz tuning fork resonators which are being increasingly used in scanning probe microscopy as force sensors. We will also show that a coupled harmonic oscillators model, which includes a finite coupling between the prongs, is in remarkable agreement with the observed motion of the tuning forks. Relevant parameters for the tuning fork performance such as the effective spring constant can be obtained from our analysis. In the second one, we will present an implementation of a quartz tuning fork supplemented with optimized tips based on carbon fibers. The remarkable electrical and mechanical properties of carbon fiber make these tips more suitable for combined and/or simultaneous STM and AFM than conventional metallic tips. The fabrication and the characterization of these carbon fiber tips as well as their performance in STM/AFM will be detailed.

  15. Effects of Engineered Log Jams on Channel Morphology, Middle Fork of the John Day River, Oregon

    NASA Astrophysics Data System (ADS)

    Duffin, J.; McDowell, P. F.

    2015-12-01

    Engineered log jams (ELJs) were constructed on the Middle Fork of the John Day River in eastern Oregon as part of a large river restoration project. These log structures were designed to address many of the restoration goals including creating scour pools, creating and maintaining a sinuous river planform, providing fish cover, and increasing complexity of fish habitat. This study uses geomorphic change detection techniques to monitor topographic change under and around the 26 log structures in two different river reaches over a six to seven year period. This study finds that the ELJs are remaining stable within the river, even following a large flood in 2011. While some pools are shallowing or shifting laterally slightly, in general they are maintaining deep pool habitat. The study provides insight into which log structure variables are most related to the patterns and amounts of aggradation and degradation. Analysis suggests that volume of aggradation is related to both porosity and volume of the log structures, while the area of aggradation was correlated with porosity, as well as, the proportion of logs within bankfull. Understanding the geomorphic changes to the riverbed in response to the placement of the ELJs can influence the design and future effectiveness of ELJs.

  16. The Sound Field around a Tuning Fork and the Role of a Resonance Box

    ERIC Educational Resources Information Center

    Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

    2015-01-01

    Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: (1) Why is a bare tuning fork such a weak emitter of sound? (2) What is the role of the…

  17. Spring constant of a tuning-fork sensor for dynamic force microscopy

    PubMed Central

    Lange, Manfred; Schmuck, Merlin; Schmidt, Nico; Möller, Rolf

    2012-01-01

    Summary We present an overview of experimental and numerical methods to determine the spring constant of a quartz tuning fork in qPlus configuration. The simple calculation for a rectangular cantilever is compared to the values obtained by the analysis of the thermal excitation and by the direct mechanical measurement of the force versus displacement. To elucidate the difference, numerical simulations were performed taking account of the real geometry including the glue that is used to mount the tuning fork. PMID:23365793

  18. Probing Liquid ^4He with Quartz Tuning Forks Using a Novel Multifrequency Lock-in Technique

    NASA Astrophysics Data System (ADS)

    Bradley, D. I.; Haley, R. P.; Kafanov, S.; Noble, M. T.; Pickett, G. R.; Tsepelin, V.; Vonka, J.; Wilcox, T.

    2016-09-01

    We report on a novel technique to measure quartz tuning forks, and possibly other vibrating objects, in a quantum fluid using a multifrequency lock-in amplifier. The multifrequency technique allows to measure the resonance curve of a vibrating object much faster than a conventional single frequency lock-in amplifier technique. Forks with resonance frequencies of 12 kHz and 16 kHz were excited and measured electro-mechanically either at a single frequency or at up to 40 different frequencies simultaneously around the same mechanical mode. The response of each fork was identical for both methods and validates the use of the multifrequency lock-in technique to probe properties of liquid helium at low fork velocities. Using both methods we measured the resonance frequency and drag of two 25-μ m-wide quartz tuning forks immersed in liquid ^4He in the temperature range from 4.2 K to 1.5 K at saturated vapour pressure. The damping and shift of resonance frequency experienced by both tuning forks at low velocities are well described by hydrodynamic contributions in the framework of the two-fluid model. The sensitivity of the 25-μ m-wide tuning forks is larger compared to similar 75-μ m-wide forks and in combination with the faster multifrequency lock-in technique could be used to improve thermometry in liquid ^4He. The multifrequency technique could also be used for studies of the onset of non-linear phenomena such as quantum turbulence and cavitation in superfluids.

  19. DNA2 drives processing and restart of reversed replication forks in human cells

    PubMed Central

    Thangavel, Saravanabhavan; Berti, Matteo; Levikova, Maryna; Pinto, Cosimo; Gomathinayagam, Shivasankari; Vujanovic, Marko; Zellweger, Ralph; Moore, Hayley; Lee, Eu Han; Hendrickson, Eric A.; Cejka, Petr; Stewart, Sheila; Lopes, Massimo

    2015-01-01

    Accurate processing of stalled or damaged DNA replication forks is paramount to genomic integrity and recent work points to replication fork reversal and restart as a central mechanism to ensuring high-fidelity DNA replication. Here, we identify a novel DNA2- and WRN-dependent mechanism of reversed replication fork processing and restart after prolonged genotoxic stress. The human DNA2 nuclease and WRN ATPase activities functionally interact to degrade reversed replication forks with a 5′-to-3′ polarity and promote replication restart, thus preventing aberrant processing of unresolved replication intermediates. Unexpectedly, EXO1, MRE11, and CtIP are not involved in the same mechanism of reversed fork processing, whereas human RECQ1 limits DNA2 activity by preventing extensive nascent strand degradation. RAD51 depletion antagonizes this mechanism, presumably by preventing reversed fork formation. These studies define a new mechanism for maintaining genome integrity tightly controlled by specific nucleolytic activities and central homologous recombination factors. PMID:25733713

  20. Replication fork arrest and termination of chromosome replication in Bacillus subtilis.

    PubMed

    Wake, R G

    1997-08-15

    Sporulation in Bacillus subtilis provided the first evidence for the presence of sequence-specific replication fork arrest (Ter) sites in the terminus region of the bacterial chromosome. These sites, when complexed with the replication terminator protein (RTP), block movement of a replication fork in a polar manner. The Ter sites are organized into two opposed groups which force the approaching forks to meet and fuse within a restricted terminus region. While the precise advantage provided to the cell through the presence of the so-called replication fork trap is not patently obvious, the same situation appears to have evolved independently in Escherichia coli. The molecular mechanism by which the RTP-Ter complex of B. subtilis (or the analogous but apparently unrelated complex in E. coli) functions is currently unresolved and subject to intense investigation. Replication fork arrest in B. subtilis, requiring RTP, also occurs under conditions of the stringent response at so-called STer sites that lie close to and on both sides of oriC. These sites are yet to be identified and characterized. How they are induced to function under stringent conditions is of considerable interest, and could provide vital clues about the mechanism of fork arrest by RTP-terminator complexes in general.

  1. Metnase promotes restart and repair of stalled and collapsed replication forks.

    PubMed

    De Haro, Leyma P; Wray, Justin; Williamson, Elizabeth A; Durant, Stephen T; Corwin, Lori; Gentry, Amanda C; Osheroff, Neil; Lee, Suk-Hee; Hromas, Robert; Nickoloff, Jac A

    2010-09-01

    Metnase is a human protein with methylase (SET) and nuclease domains that is widely expressed, especially in proliferating tissues. Metnase promotes non-homologous end-joining (NHEJ), and knockdown causes mild hypersensitivity to ionizing radiation. Metnase also promotes plasmid and viral DNA integration, and topoisomerase IIα (TopoIIα)-dependent chromosome decatenation. NHEJ factors have been implicated in the replication stress response, and TopoIIα has been proposed to relax positive supercoils in front of replication forks. Here we show that Metnase promotes cell proliferation, but it does not alter cell cycle distributions, or replication fork progression. However, Metnase knockdown sensitizes cells to replication stress and confers a marked defect in restart of stalled replication forks. Metnase promotes resolution of phosphorylated histone H2AX, a marker of DNA double-strand breaks at collapsed forks, and it co-immunoprecipitates with PCNA and RAD9, a member of the PCNA-like RAD9-HUS1-RAD1 intra-S checkpoint complex. Metnase also promotes TopoIIα-mediated relaxation of positively supercoiled DNA. Metnase is not required for RAD51 focus formation after replication stress, but Metnase knockdown cells show increased RAD51 foci in the presence or absence of replication stress. These results establish Metnase as a key factor that promotes restart of stalled replication forks, and implicate Metnase in the repair of collapsed forks.

  2. Symmetrically arranged quartz tuning fork with soft cantilever for intermittent contact mode atomic force microscopy

    NASA Astrophysics Data System (ADS)

    Akiyama, T.; Staufer, U.; de Rooij, N. F.; Frederix, P.; Engel, A.

    2003-01-01

    A self-sensing and -actuating probe for dynamic mode atomic force microscopy (AFM) based on a commercial quartz tuning fork and a microfabricated cantilever is presented. The U-shaped cantilever, exhibiting a sharp tip, is combined with the tuning fork in a symmetrical arrangement, such that each of the two legs of the cantilever is fixed to one of the prongs of the tuning fork. The tuning fork is used as an oscillatory force sensor. Its frequency and amplitude govern that of the tip vibration, while the cantilever determines the spring constant of the whole probe. The frequency of the tip vibration for AFM operations can be much higher than the resonance frequency of the cantilever. A probe comprising a silicon nitride cantilever (0.1 N/m) is used to image monoatomic terraces of graphite in the intermittent contact mode. A much softer cantilever (0.01 N/m) is used to analyze the topography of a microelectronic chip in the same mode. Moreover, a bacterial surface layer hexagonally packed intermediate layer of Deinococcus radiodurans is imaged in a buffer solution. The tip vibration was again generated by the tuning fork while the sample interaction was measured using the standard optical detection scheme in this experiment. These probes are suited for batch fabrication and assembly and, therefore, enlarge the applications for the tuning fork based AFM.

  3. RecG interacts directly with SSB: implications for stalled replication fork regression.

    PubMed

    Buss, Jackson A; Kimura, Yuji; Bianco, Piero R

    2008-12-01

    RecG and RuvAB are proposed to act at stalled DNA replication forks to facilitate replication restart. To define the roles of these proteins in fork regression, we used a combination of assays to determine whether RecG, RuvAB or both are capable of acting at a stalled fork. The results show that RecG binds to the C-terminus of single-stranded DNA binding protein (SSB) forming a stoichiometric complex of 2 RecG monomers per SSB tetramer. This binding occurs in solution and to SSB protein bound to single stranded DNA (ssDNA). The result of this binding is stabilization of the interaction of RecG with ssDNA. In contrast, RuvAB does not bind to SSB. Side-by-side analysis of the catalytic efficiency of the ATPase activity of each enzyme revealed that (-)scDNA and ssDNA are potent stimulators of the ATPase activity of RecG but not for RuvAB, whereas relaxed circular DNA is a poor cofactor for RecG but an excellent one for RuvAB. Collectively, these data suggest that the timing of repair protein access to the DNA at stalled forks is determined by the nature of the DNA available at the fork. We propose that RecG acts first, with RuvAB acting either after RecG or in a separate pathway following protein-independent fork regression.

  4. Evaluating the effectiveness of floodplain restoration on the North Fork John Day River, Northeast Oregon, USA

    NASA Astrophysics Data System (ADS)

    Clifton, C. F.; Blanton, P.; Long, W.; Walterman, M. T.; McDowell, P. F.; Maus, P.

    2007-12-01

    Over the last decade hundreds of river restoration projects intended to maintain, protect, and restore watersheds, rivers, and habitat for native species in the Pacific Northwest have been implemented. By some counts, investment in watershed restoration exceeds hundreds of millions of dollars annually yet the effectiveness of these efforts remains an elusive question (Roni, 2005). Remote sensing and GIS technologies show great promise for large-scale river monitoring, however most natural resource organizations who implement these projects have limited budget and staff and would benefit from simple, low cost monitoring techniques that use readily available imagery. We used 1:24000 digitized orthorectified resource imagery from 1995, and National Agriculture Imagery Program (NAIP) digital orthophotography from 2005 to assess the effectiveness of floodplain restoration on a 16 km reach of the North Fork John Day River. Between 1993 and 1997 this section was restored by mechanically removing, reshaping, and revegetating cobble-boulder tailings piles left from dredge mining. The project was intended to directly improve floodplain function (i.e. inundation, riparian habitat) and indirectly improve instream habitat (pools, spawning) by reconnecting the active river channel with a reconstructed floodplain surface. Project effectiveness was not well documented initially in terms of quantifying floodplain functional area improvement or channel condition and response at the river-reach scale. Our objectives were to field-verify remote sensing measurements of response variables to test the applicability of available remote sensing imagery for project effectiveness monitoring, and to quantify adjustment in river response variables, using a "before-after" case study approach. Bracketing restoration activities with 1995 and 2000 imagery, we developed and tested methods for acquisition and processing of digital imagery and identified a core set of response variables to sample

  5. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils

    SciTech Connect

    1994-09-01

    The Lower East Fork Poplar Creek (LEFPC) extends fourteen (14) miles through Oak Ridge, TN. The Creek sediments and surrounding floodplain soils are contaminated with mercury compounds. This project involved a comprehensive pilot demonstration on thermal desorption of these soils to validate the feasibility of the remedial technology which had been identified in previous studies. Thermal desorption is a technology that utilizes heating or drying of soils to induce volatilization of contaminants. These contaminants are then vaporized and either incinerated or condensed in the second stage of desorption. Mercury (Hg), which was the principal contaminate of concern, was collected by condensers in a vapor collection system. This type of system insured that the toxic mercury vapors did not escape to the atmosphere.

  6. Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork.

    PubMed

    Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

    2015-06-01

    A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr(1-x)Ti(x))O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever. PMID:26133877

  7. Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonaradian Age) Reservoirs, West Texas and New Mexico

    SciTech Connect

    Philip, Zeno; Jennings, Jr., James W.

    2001-05-08

    The major goal of this project was to evaluate the impact of fracture porosity on performance of the South Wasson Clear Fork reservoir. The approach was to use subcritical crack (SCC) index measurements and a crack-growth simulator to model potential fracture geometries in this reservoir. The SCC index on representative rock samples and proceedings with other pertinent rock measurements were measured. An approach for modeling coupled matrix and fracture flow using nonneighbor connections in a traditional finite-difference simulator was tested and found to be feasible.

  8. Reconceptualizing University Extension and Public Service. [and] University Extension and Public Service in the Age of Economic Globalization: A Response to Thompson and Lamble.

    ERIC Educational Resources Information Center

    Thompson, Gordon; Lamble, Wayne; Lauzon, Allan C.

    2000-01-01

    Thompson and Lamble describe the evolution, role, and characteristics of university extension, identifying uncertainty and confusion about the field. They compare community and instructional orientations and synthesize concepts of extension and public service. Lauzon's response suggests the need to account for the changing context of university…

  9. Warts and All: Personal Reflections on Social Justice in Counseling Psychology. Reply to Kiselica, Palmer, Thompson and Shermis, and Watts. Rejoinder

    ERIC Educational Resources Information Center

    Goodman, Lisa A.; Liang, Belle; Weintraub, Sarah R.; Helms, Janet E.; Latta, Rachel E.

    2004-01-01

    In their comments regarding Goodman, Liang, Helms, Latta, Sparks, and Weintraub's article "Training Counseling Psychologists as Social Justice Agents: Feminist and Multicultural Principles in Action," Kiselica, Palmer, Thompson and Shermis, and Watts offered a number of useful suggestions for elaborating on social justice theory, practice, and…

  10. "It Is Better to Light a Candle than to Curse the Darkness": Ethel Thompson Overby and Democratic Schooling in Richmond, Virginia, 1910-1958

    ERIC Educational Resources Information Center

    Ward Randolph, Adah L.

    2012-01-01

    In 1933, Ethel Thompson Overby became the first African American female principal in Richmond, Virginia. Her motto was "It is better to light a candle than to curse the darkness" (Overby 1975, 1). Before becoming principal, Overby had been a teacher in the southern urban "de jure" segregated schools of the city. How did the racially segregated…

  11. Carbon Dioxide, Hydrographic, and Chemical Data Obtained During the R/V Thomas G. Thompson Cruise in the Pacific Ocean

    SciTech Connect

    Sabine, C.L.; Key, R.M.; Hall, M.; Kozyr, A.

    1999-08-01

    This data documentation discusses the procedures and methods used to measure total carbon dioxide (TCO2), total alkalinity (TALK), and radiocarbon (delta 14C), at hydrographic stations, as well as the underway partial pressure of CO2 (pCO2) during the R/V Thomas G. Thompson oceanographic cruise in the Pacific Ocean (Section P10). Conducted as part of the World Ocean Circulation Experiment (WOCE), the cruise began in Suva, Fiji, on October 5, 1993, and ended in Yokohama, Japan, on November 10, 1993. Measurements made along WOCE Section P10 included pressure, temperature, salinity [measured by conductivity temperature, and depth sensor (CTD)], bottle salinity, bottle oxygen, phosphate, nitrate, silicate, chlorofluorocarbons (CFC-11, CFC-12), TCO2, TALK, delta 14C, and underway pCO2.

  12. Characteristics of Subcooled Liquid Methane During Passage Through a Spray-Bar Joule-Thompson Thermodynamic Vent System

    NASA Technical Reports Server (NTRS)

    Hastings, L. J.; Bolshinskiy, L. G.; Hedayat, A.; Schnell, A.

    2011-01-01

    NASA s Marshall Space Flight Center (MSFC) conducted liquid methane (LCH4) testing in November 2006 using the multipurpose hydrogen test bed (MHTB) outfitted with a spray-bar thermodynamic vent system (TVS). The basic objective was to identify any unusual or unique thermodynamic characteristics associated with subcooled LCH4 that should be considered in the design of space-based TVSs. Thirteen days of testing were performed with total tank heat loads ranging from 720 W to 420 W at a fill level of approximately 90%. During an updated evaluation of the data, it was noted that as the fluid passed through the Joule Thompson expansion, thermodynamic conditions consistent with the pervasive presence of metastability were indicated. This paper describes the observed thermodynamic conditions that correspond with metastability and effects on TVS performance.

  13. Autonomy of ascidian fork head/HNF-3 gene expression.

    PubMed

    Shimauchi, Y; Yasuo, H; Satoh, N

    1997-12-01

    We have characterized the expression pattern of a class I fork head/HNF-3 gene (HrHNF3-1) of the ascidian Halocynthia roretzi. Zygotic HrHNF3-1 expression was detectable as early as the 16-cell stage, and the transcript was evident in blastomeres of the endoderm, notochord and mesenchyme lineages of the early embryos. After the late gastrula stage, HrHNF3-1 was also expressed in the presumptive spinal cord cells and some brain cells. The spinal cord of the ascidian tadpole consists of four layers of cells; the dorsal layer, two lateral layers and the ventral layer, the latter of which simply lies on the notochord. Cross-sections of in situ hybridized specimens showed that HrHNF3-1 was expressed in cells of the ventral layer, reminiscent of the floor plate of vertebrate embryos. In addition, we found autonomy in the initiation of early HrHNF3-1 expression, because the gene was expressed in blastomeres continuously dissociated from the first cleavage until the 16-cell stage.

  14. CDK1 phosphorylates WRN at collapsed replication forks

    PubMed Central

    Palermo, Valentina; Rinalducci, Sara; Sanchez, Massimo; Grillini, Francesca; Sommers, Joshua A.; Brosh, Robert M.; Zolla, Lello; Franchitto, Annapaola; Pichierri, Pietro

    2016-01-01

    Regulation of end-processing is critical for accurate repair and to switch between homologous recombination (HR) and non-homologous end joining (NHEJ). End resection is a two-stage process but very little is known about regulation of the long-range resection, especially in humans. WRN participates in one of the two alternative long-range resection pathways mediated by DNA2 or EXO1. Here we demonstrate that phosphorylation of WRN by CDK1 is essential to perform DNA2-dependent end resection at replication-related DSBs, promoting HR, replication recovery and chromosome stability. Mechanistically, S1133 phosphorylation of WRN is dispensable for relocalization in foci but is involved in the interaction with the MRE11 complex. Loss of WRN phosphorylation negatively affects MRE11 foci formation and acts in a dominant negative manner to prevent long-range resection altogether, thereby licensing NHEJ at collapsed forks. Collectively, we unveil a CDK1-dependent regulation of the WRN-DNA2-mediated resection and identify an undescribed function of WRN as a DSB repair pathway switch. PMID:27634057

  15. CDK1 phosphorylates WRN at collapsed replication forks.

    PubMed

    Palermo, Valentina; Rinalducci, Sara; Sanchez, Massimo; Grillini, Francesca; Sommers, Joshua A; Brosh, Robert M; Zolla, Lello; Franchitto, Annapaola; Pichierri, Pietro

    2016-01-01

    Regulation of end-processing is critical for accurate repair and to switch between homologous recombination (HR) and non-homologous end joining (NHEJ). End resection is a two-stage process but very little is known about regulation of the long-range resection, especially in humans. WRN participates in one of the two alternative long-range resection pathways mediated by DNA2 or EXO1. Here we demonstrate that phosphorylation of WRN by CDK1 is essential to perform DNA2-dependent end resection at replication-related DSBs, promoting HR, replication recovery and chromosome stability. Mechanistically, S1133 phosphorylation of WRN is dispensable for relocalization in foci but is involved in the interaction with the MRE11 complex. Loss of WRN phosphorylation negatively affects MRE11 foci formation and acts in a dominant negative manner to prevent long-range resection altogether, thereby licensing NHEJ at collapsed forks. Collectively, we unveil a CDK1-dependent regulation of the WRN-DNA2-mediated resection and identify an undescribed function of WRN as a DSB repair pathway switch. PMID:27634057

  16. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    SciTech Connect

    ADAMS, S.M.; ASHWOOD, T.L.; BEATY, T.W.; BRANDT, C.C.

    1997-10-24

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y- 12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  17. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    SciTech Connect

    ADAMS, S.M.; BEATY, T.W.; BRANDT, C.C.; CHRISTENSEN, S.W.; CICERONE, D.S.

    1998-09-09

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  18. Biological monitoring program for East Fork Poplar Creek

    SciTech Connect

    Adams, S.M.; Ashwood, T.L.; Beaty, T.W.; Brandt, C.C.; Christensen, S.W.; Cicerone, D.S.; Greeley, M.S. Jr.; Hill, W.R.; Kszos, L.S.

    1997-04-18

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  19. Tuning Fork Oscillators as Downhole Viscometers in Oilfield Applications

    NASA Astrophysics Data System (ADS)

    Gonzalez, Miguel; Bernero, Greg; Alvarez, Oliverio; Ham, Gregory; Max, Deffenbaugh; Sensors Development Team

    2015-03-01

    The commerciality of oil wells is greatly influenced by the physical properties of the fluids being produced. A key parameter in determining how producible the hydrocarbons are is their viscosity. Pressure and temperature changes in recovering a downhole sample to the surface can alter viscosity while accurate downhole measurement of this critical property remains a rudimentary effort in the industry. In this presentation we describe the challenges of measuring and quantifying the viscosity of reservoir fluids in situ at downhole conditions, as well as present an overview of some of the different measurement techniques currently used. Additionally, we show our characterization of a piezoelectric tuning fork oscillator used as a viscosity sensor. In an attempt to recreate the environment found in oil wells, its mechanical and electrical properties were studied while the device was immersed in different fluids and, separately, under different conditions of pressure and temperature. This device is a first step toward the development of an inexpensive, integrated, and miniaturized sensing platform for the in situ characterization of reservoir fluids.

  20. Geomorphic Effects of Engineered Log Jams in River Restoration, Middle Fork John Day River

    NASA Astrophysics Data System (ADS)

    Duffin, J.; McDowell, P. F.

    2014-12-01

    The Middle Fork of the John Day River (MFJD) Intensively Monitored Watershed in eastern Oregon is a multi-phase restoration implementation and monitoring project. MFJD is a tributary to the Colombia and is part of one of the longest free flowing rivers systems in the continental United States. It is a gravel and cobble bed river with a drainage area of 2,100 km2. The river has endured extensive channel and floodplain degradation from years of channel alteration and straightening due to human influences including dredge mining, ranching, and farming. As part of the river restoration project on the MFJD, engineered log jams have been constructed to address many of the restoration goals including creating scour pools, inhibiting bank erosion, creating and maintaining a sinuous river planform, and increasing complexity of fish habitat. There is a need for more detailed understanding on ELJ channel morphologic effects and how site-specific characteristics and differences in log jam infrastructure interact to create the in-channel features over timescales longer than a few years. This study uses detailed channel bed topographic surveys collected either with a total station or RTK-GPS technology. Geomorphic change detection techniques are utilized to monitor topographic change under and around the 26 log structures in two different river reaches over a six to seven year period The log structures are often associated with deepening of pools as desired, but also some structures show sedimentation under the structure. Differences in the patterns will be assessed based on the design, location, and specific characteristics of the log structures; variables include number and placement of logs, volume of structure, location on meander bend, and sediment sizes.

  1. Tim/Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway.

    PubMed

    Calì, Federica; Bharti, Sanjay Kumar; Di Perna, Roberta; Brosh, Robert M; Pisani, Francesca M

    2016-01-29

    We present evidence that Tim establishes a physical and functional interaction with DDX11, a super-family 2 iron-sulfur cluster DNA helicase genetically linked to the chromosomal instability disorder Warsaw breakage syndrome. Tim stimulates DDX11 unwinding activity on forked DNA substrates up to 10-fold and on bimolecular anti-parallel G-quadruplex DNA structures and three-stranded D-loop approximately 4-5-fold. Electrophoretic mobility shift assays revealed that Tim enhances DDX11 binding to DNA, suggesting that the observed stimulation derives from an improved ability of DDX11 to interact with the nucleic acid substrate. Surface plasmon resonance measurements indicate that DDX11 directly interacts with Tim. DNA fiber track assays with HeLa cells exposed to hydroxyurea demonstrated that Tim or DDX11 depletion significantly reduced replication fork progression compared to control cells; whereas no additive effect was observed by co-depletion of both proteins. Moreover, Tim and DDX11 are epistatic in promoting efficient resumption of stalled DNA replication forks in hydroxyurea-treated cells. This is consistent with the finding that association of the two endogenous proteins in the cell extract chromatin fraction is considerably increased following hydroxyurea exposure. Overall, our studies provide evidence that Tim and DDX11 physically and functionally interact and act in concert to preserve replication fork progression in perturbed conditions.

  2. BLM SUMOylation regulates ssDNA accumulation at stalled replication forks.

    PubMed

    Ouyang, Karen J; Yagle, Mary K; Matunis, Michael J; Ellis, Nathan A

    2013-01-01

    Polymerase stalling results in uncoupling of DNA polymerase and the replicative helicase, which generates single-stranded DNA (ssDNA). After stalling, RAD51 accumulates at stalled replication forks to stabilize the fork and to repair by homologous recombination (HR) double-strand breaks (DSBs) that accumulate there. We showed recently that SUMO modification of the BLM helicase is required in order for RAD51 to accumulate at stalled forks. In order to investigate how BLM SUMOylation controls RAD51 accumulation, we characterized the function of HR proteins and ssDNA-binding protein RPA in cells that stably expressed either normal BLM (BLM+) or SUMO-mutant BLM (SM-BLM). In HU-treated SM-BLM cells, mediators BRCA2 and RAD52, which normally substitute RAD51 for RPA on ssDNA, failed to accumulate normally at stalled forks; instead, excess RPA accumulated. SM-BLM cells also exhibited higher levels of HU-induced chromatin-bound RPA than BLM+ cells did. The excess RPA did not result from excessive intrinsic BLM helicase activity, because in vitro SUMOylated BLM unwound similar amounts of replication-fork substrate as unSUMOylated BLM. Nor did BLM SUMOylation inhibit binding of RPA to BLM in vitro; however, in immunoprecipitation experiments, more BLM-RPA complex formed in HU-treated SM-BLM cells, indicating that BLM SUMOylation controls the amount of BLM-RPA complex normally formed at stalled forks. Together, these results showed that BLM SUMOylation regulates the amount of ssDNA that accumulates during polymerase stalling. We conclude that BLM SUMOylation functions as a licensing mechanism that permits and regulates HR at damaged replication forks.

  3. [Heritability of body weight and fork length for Oncorhynchus masou masou].

    PubMed

    Zhang, Yu-Yong; Jia, Zhi-Ying; Bai, Qing-Li; Chen, Shu-Qiang; Shi, Lian-Yu; Wang, Bing-Qian

    2013-02-01

    Body weight and body length have been considered as the most important production traits for the fish genetic improvement. For cold-water fish, body length was usually substituted by fork length. In order to estimate the heritability of body weight and fork length of the sixth generation Oncorhynchus masou masou, which was introduced into China, the method of unbalanced nest design and an artificial insemination technigue were used. Twenty-nine full-sib families and fourteen half-sib families were obtained. Body weight and fork length of O. masou masou were measured in 12 and 24 months after fertilization. Based on full-sib and half-sib families data, the causal components of phenotypic variance were calculated. The results showed that, (1) during the whole growth phase of O. masou masou, the coefficient variation (CV) of fork length was higher than body weight, and CV of 12-month old was higher than that of 24-month old; (2) body weight and fork length of O. masou masou among sires and dams among sires were significant difference (P<0.01) both at 12 months and at 24 months; (3) the maternal component estimates were significantly larger than those of paternal ones for body weight and fork length traits both at 12 months and at 24 months; (4) for 12 months of O. masou masou the heritabilities of body weight and fork length were 0.41~0.51 and 0.46~0.54, respectively. For 24 months the values were 0.55~0.60 and 0.53~0.59, respectively; and (5) it was concluded that the heritability of growth traits in O. masou masou was relatively high and this highlights the potential to improve its growth through selective breeding. This study shows important data supporting for further genetic improvement of O. masou masou. PMID:23448933

  4. Maintaining replication fork integrity in UV-irradiated Escherichia coli cells.

    PubMed

    Rudolph, Christian J; Upton, Amy L; Lloyd, Robert G

    2008-09-01

    In dividing cells, the stalling of replication fork complexes by impediments to DNA unwinding or by template imperfections that block synthesis by the polymerase subunits is a serious threat to genomic integrity and cell viability. What happens to stalled forks depends on the nature of the offending obstacle. In UV-irradiated Escherichia coli cells DNA synthesis is delayed for a considerable period, during which forks undergo extensive processing before replication can resume. Thus, restart depends on factors needed to load the replicative helicase, indicating that the replisome may have dissociated. It also requires the RecFOR proteins, which are known to load RecA recombinase on single-stranded DNA, implying that template strands are exposed. To gain a further understanding of how UV irradiation affects replication and how replication resumes after a block, we used fluorescence microscopy and BrdU or radioisotope labelling to examine chromosome replication and cell cycle progression. Our studies confirm that RecFOR promote efficient reactivation of stalled forks and demonstrate that they are also needed for productive replication initiated at the origin, or triggered elsewhere by damage to the DNA. Although delayed, all modes of replication do recover in the absence of these proteins, but nascent DNA strands are degraded more extensively by RecJ exonuclease. However, these strands are also degraded in the presence of RecFOR when restart is blocked by other means, indicating that RecA loading is not sufficient to stabilise and protect the fork. This is consistent with the idea that RecA actively promotes restart. Thus, in contrast to eukaryotic cells, there may be no factor in bacterial cells acting specifically to stabilise stalled forks. Instead, nascent strands may be protected by the simple expedient of promoting restart. We also report that the efficiency of fork reactivation is not affected in polB mutants. PMID:18644471

  5. Mercury Contributions from Flint Creek and other Tributaries to the Upper Clark Fork River in Northwestern Montana

    NASA Astrophysics Data System (ADS)

    Langner, H.; Young, M.; Staats, M. F.

    2013-12-01

    Methylmercury contamination in biota is a major factor diminishing the environmental quality of the Upper Clark Fork River (CFR), e.g. by triggering human consumption limits of fish. The CFR is subject to one of the largest Superfund cleanup projects in the US, but remediation and restoration is currently focused exclusively on other mining-related contaminants (As, Cu, Zn, Pb, Cd), which may be counterproductive with respect to the bio-availability of mercury, for example by creation of wetlands along mercury-contaminated reaches of the river. The identification and elimination of Hg sources is an essential step toward reducing the methylmercury exposure in the biota of the CFR watershed because a strong correlation exists between total mercury levels in river sediment and methylmercury levels in aquatic life. We analyzed duplicate samples from the top sediment layer of the main stem and significant tributaries to the Clark Fork River along a 240 km reach between Butte, MT and downstream of the Missoula Valley. Mercury concentrations were 1.3 × 1.6 (mean × SD, n = 35) in the main stem. Concentrations in tributaries varied widely (0.02 to 85 mg/kg) and seemed only loosely related to the number of historic precious metal mines in the watershed. In the upper reach of the CFR, elevated Hg levels are likely caused by residual contaminated sediments in the flood plain. Levels tend to decrease downstream until Drummond, MT, where Flint Creek contributes a significant amount of mercury, causing Hg levels in the main stem CFR to increase from 0.7 to 4 mg/kg. Levels continue to decrease downstream. Flint Creek is the single largest contributor of Hg to the CFR. Detailed sampling of the main stem Flint Creek and tributaries (26 sites) showed extremely high levels in two tributaries (22 to 85 mg/kg) where historic milling operations were located. Elimination of these point sources may be accomplished comparatively economically and may significantly reduce mercury levels in

  6. Two Dimensional Modeling of the Extent and Stability of Cold Water Refugia at the Confluences of the South Fork of the Eel River

    NASA Astrophysics Data System (ADS)

    Greer, G.; Thompson, S. E.

    2015-12-01

    Like many large rivers draining from forested uplands, the South Fork of the Eel River is characterized by a warm main stem fed by small cold water tributaries along its length. The confluences of the tributaries with the main stem form localized zones of cold water which provide habitat for anadromous fish species such as salmon. These cold water refugia are likely to be increasingly important for fish as temperatures warm and rainfall becomes more erratic due to projected climate change. These sites are protected from drying out over summer (unlike the tributaries, which can fragment if flows become too low), and they are accessible to migrating fish (as tributaries may not be if winter flows are too low to submerge in-channel obstacles). The overall significance of these refugia, however, is controlled by their spatial extent and temporal persistence. The factors that determine the shape, persistence, and stability of these refugia (as a function of varying flow and temperature regimes) remain poorly understood. A combination of scaling arguments and a 2D model is used to estimate the effects of varying flow and thermal regimes on the spatial extent of the cold water zones in the main stem. The stability of the cold water zones is also assessed in light of diurnal flow and temperature variations. The results are compared to in situ temperature data collected at the confluence of the South Fork of the Eel River and Elder Creek, and are used to project the likely significance of tributary confluences for generating cold-water habitat throughout the South Fork of the Eel River.

  7. Biological Monitoring Program for East Fork Poplar Creek

    SciTech Connect

    Adams, S.M.; Christensen, S.W.; Greeley, M.S.jr; Hill, W.R.; Kszos, L.A.; McCarthy, J.F.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.

    1998-10-15

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biologicai Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the compiex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC, These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumuiation studies, and (4) ecological surveys of the periphyton, benthic macro invertebrate, and fish communities. Monitoring is currently being conducted at five sites, although sites maybe excluded and/or others added depending upon the specific objectives of the various tasks. Criteria used in selecting the sites include: (1) location of sampling sites used in other studies, (2) known or suspected sources of downstream impacts, (3) proximity to U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR) boundaries, (4) concentration of mercury in the adjacent floodplain, (5) appropriate habitat distribution, and (6

  8. Replication fork bypass of a pyrimidine dimer blocking leading strand DNA synthesis.

    PubMed

    Cordeiro-Stone, M; Zaritskaya, L S; Price, L K; Kaufmann, W K

    1997-05-23

    We constructed a double-stranded plasmid containing a single cis, syn-cyclobutane thymine dimer (T[c,s]T) 385 base pairs from the center of the SV40 origin of replication. This circular DNA was replicated in vitro by extracts from several types of human cells. The dimer was placed on the leading strand template of the first replication fork to encounter the lesion. Two-dimensional gel electrophoresis of replication intermediates documented the transient arrest of the replication fork by the dimer. Movement of the replication fork beyond the dimer was recognized by the appearance of a single fork arc in DNA sequences located between the T[c,s]T and the half-way point around the circular template (180 degrees from the origin). Upon completion of plasmid replication, the T[c,s]T was detected by T4 endonuclease V in about one-half (46 +/- 9%) of the closed circular daughter molecules. Our results demonstrate that extracts prepared from HeLa cells and SV40-transformed human fibroblasts (SV80, IDH4), including a cell line defective in nucleotide-excision repair (XPA), were competent for leading strand DNA synthesis opposite the pyrimidine dimer and replication fork bypass. In contrast, dimer bypass was severely impaired in otherwise replication-competent extracts from two different xeroderma pigmentosum variant cell lines.

  9. A study of materials at high temperature using miniaturized resonant tuning forks and noncontact capacitance transducers

    SciTech Connect

    Schindel, D.W.; Hutchins, D.A.; Smith, S.T.

    1997-09-01

    A noncontact acoustical system has been developed which measures Young`s modulus of solid materials at high temperature with {le}0.05{percent} accuracy. The system employs capacitive (or electrostatic) transducers to excite and detect vibrations of millimeter-sized resonant tuning forks, whose resonance frequencies alter with changing temperature and material properties. The use of tuning forks in their fundamental symmetric modes of vibration provide resonances of high Q`s and eliminate irreversible frequency and drift effects that occur with other forms of resonator. The use of noncontact capacitive transducers reduces the damping and stresses that otherwise occur with contacting transducers, and allows the system to be simply and accurately modeled. Both single crystal silicon tuning forks, and those manufactured from hydrided and unhydrided Zr{endash}2.5{percent}Nb, were investigated at temperatures up to 700{degree}C. The measured responses of silicon forks confirmed the system accuracy, and suggested that single-crystal silicon be standardized as a calibration material for acoustical measurement systems. Results obtained with Zr{endash}2.5{percent}Nb tuning forks allowed an accurate quantitative analysis of the effects of hydride dissolution and precipitation on Young`s modulus, and confirmed that Young`s modulus of hydrided Zr{endash}2.5{percent}Nb decreases in proportion to free hydrogen concentration. This experimental system should prove valuable for accurate modeling of high-temperature material transformations in solids. {copyright} {ital 1997 Acoustical Society of America.}

  10. Quartz tuning-fork oscillations in He II and drag coefficient

    NASA Astrophysics Data System (ADS)

    Gritsenko, I. A.; Zadorozhko, A. A.; Neoneta, A. S.; Chagovets, V. K.; Sheshin, G. A.

    2011-07-01

    The temperature dependencies of drag coefficient for quartz tuning forks of various geometric dimensions, immersed in the He II, were determined experimentally in the temperature range 0.1-3 K. It is identified, that these dependencies are similar, but the values of drag coefficient are different for tuning forks with different geometric dimensions. It is shown, that the obtained specific drag coefficient depends only on the temperature and frequency of vibrations, when the value of drag coefficient is normalized to the surface area of moving tuning-fork prong. The temperature dependencies of normalized drag coefficient for the tuning forks of various dimensions, wire, and microsphere, oscillating in the Не II, are compared. It is shown, that in the ballistic regime of scattering of quasiparticles, these dependencies are identical and have a slope proportional to T4, which is determined by the density of thermal excitations. In the hydrodynamic regime at T > 0.5 K, the behavior of the temperature dependence of specific drag coefficient is affected by the size and frequency of vibrating body. The empirical relation, which allows to describe the behavior of specific drag coefficient for vibrating tuning forks, microsphere, and wire everywhere over the temperature region and at various frequencies, is proposed.

  11. BWR spent-fuel measurements with the ION-1/fork detector and a calorimeter

    SciTech Connect

    Rinard, P.M.; Bosler, G.E.

    1986-08-01

    Gamma-ray and neutron measurements were made on about 50 irradiated boiling-water reactor (BWR) fuel assemblies using the Los Alamos National Laboratory ION-1/fork detector. The assemblies were placed in a dry storage cask (DOE's REA-2023) at the General Electric Morris Operation (GE-MO) as part of a program to evaluate the cask performance. Battelle Pacific Northwest Laboratory (PNL) conducted the program. PNL compared axial radiation profiles developed from ION-1/fork measurements with calculated profiles to interpret the temperature distributions within the cask. The gamma-ray profiles correlated with heat-emission rates measured with a calorimeter, which suggests that the ION-1/fork detector is much faster than the more direct calorimeter. In addition, the radiation profiles from the ION-1/fork detector can prevent cask loadings with undesirable heat source distributions. The detector also provides safeguards information by verifying the declared exposures and cooling times. The genuineness of the assemblies is thus confirmed just before the filling and sealing of a cask. The ION-1/fork detector was permanently installed in the GE-MO fuel storage pond for 1 year without any breakdowns or significant maintenance required. Data were gathered for 9 months and analyzed using techniques developed during previous measurement campaigns. A few anomalies were found in generally satisfactory results. The detector's ease of use, reliability, and reproducibility were excellent.

  12. Tuning fork test utilization in detection of fractures: a review of the literature

    PubMed Central

    Kazemi, Mohsen

    1999-01-01

    A review of the literature was conducted to find relevant publications on the validity, reliability and utilization of the tuning fork test in detection of stress and simple fractures. Medline 1966-1998, Cumulative Index to Nursing and Allied Health Literature (CINAHL) 1982-1997, Science Citation Index 1961-1997, Index to chiropractic literature 1980-1998 and Chiropractic Research Archives Collection 1984-1990 data bases were searched. Key words such as tuning fork, vibration, diapason, fracture, stress fracture were used. The literature regarding the utilization of the tuning fork test in detection of fractures is very scarce. There was no study found in the above data bases on the validity and or reliability of the tuning fork test in detection of simple acute fractures. This review of the literature indicates the necessity of such a study since the tuning fork test has been used on the field for diagnosis of simple acute fractures. Imagesp124-ap124-bp124-cp124-dp124-e

  13. Replication fork blockage by RTS1 at an ectopic site promotes recombination in fission yeast.

    PubMed

    Ahn, Jong Sook; Osman, Fekret; Whitby, Matthew C

    2005-06-01

    Homologous recombination is believed to play important roles in processing stalled/blocked replication forks in eukaryotes. In accordance with this, recombination is induced by replication fork barriers (RFBs) within the rDNA locus. However, the rDNA locus is a specialised region of the genome, and therefore the action of recombinases at its RFBs may be atypical. We show here for the first time that direct repeat recombination, dependent on Rad22 and Rhp51, is induced by replication fork blockage at a site-specific RFB (RTS1) within a 'typical' genomic locus in fission yeast. Importantly, when the RFB is positioned between the direct repeat, conservative gene conversion events predominate over deletion events. This is consistent with recombination occurring without breakage of the blocked fork. In the absence of the RecQ family DNA helicase Rqh1, deletion events increase dramatically, which correlates with the detection of one-sided DNA double-strand breaks at or near RTS1. These data indicate that Rqh1 acts to prevent blocked replication forks from collapsing and thereby inducing deletion events.

  14. Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells

    PubMed Central

    Kaushik Tiwari, Meetu; Adaku, Nneoma; Peart, Natoya; Rogers, Faye A.

    2016-01-01

    Structural alterations in DNA can serve as natural impediments to replication fork stability and progression, resulting in DNA damage and genomic instability. Naturally occurring polypurine mirror repeat sequences in the human genome can create endogenous triplex structures evoking a robust DNA damage response. Failures to recognize or adequately process these genomic lesions can result in loss of genomic integrity. Nucleotide excision repair (NER) proteins have been found to play a prominent role in the recognition and repair of triplex structures. We demonstrate using triplex-forming oligonucleotides that chromosomal triplexes perturb DNA replication fork progression, eventually resulting in fork collapse and the induction of double strand breaks (DSBs). We find that cells deficient in the NER damage recognition proteins, XPA and XPC, accumulate more DSBs in response to chromosomal triplex formation than NER-proficient cells. Furthermore, we demonstrate that XPC-deficient cells are particularly prone to replication-associated DSBs in the presence of triplexes. In the absence of XPA or XPC, deleterious consequences of triplex-induced genomic instability may be averted by activating apoptosis via dual phosphorylation of the H2AX protein. Our results reveal that damage recognition by XPC and XPA is critical to maintaining replication fork integrity and preventing replication fork collapse in the presence of triplex structures. PMID:27298253

  15. Regional Ecorisk Field investigation, upper Clark Fork River Basin

    SciTech Connect

    Pastorok, R.; LaTier, A.; Ginn, T.

    1995-12-31

    The Regional Ecorisk Field Investigation was conducted at the Clark Fork River Superfund Site (Montana) to evaluate the relationships between plant communities and tailings deposits in riparian habitats and to evaluate food-chain transfer of trace elements to selected wildlife species. Stations were selected to represent a range of vegetation biomass (or cover) values and apparent impact of trace elements, with some areas of lush vegetation, some areas of mostly unvegetated soil (e.g., < 30 percent plant cover), and a gradient in between. For the evaluation of risk to wildlife, bioaccumulation of metals was evaluated in native or naturalized plants, terrestrial invertebrates, and the deer mouse (Peromyscus maniculatus). Potential reproductive effects in the deer mouse were evaluated by direct measurements. For other wildlife species, bioaccumulation data were interpreted in the context of food web exposure models. Total biomass and species richness of riparian plant communities are related to tailings content of soil as indicated by pH and metals concentrations. Risk to populations of omnivorous small mammals such as the deer mouse was not significant. Relative abundance and reproductive condition of the deer mouse were normal, even in areas of high metals enrichment. Based on exposure models and site-specific tissue residue data for dietary species, risk to local populations of predators such as red fox and American kestrel that feed on deer mice and terrestrial invertebrates is not significant. Risk to herbivores related to metals bioaccumulation in plant tissues is not significant. Population level effects in deer and other large wildlife are not expected because of the large home ranges of such species and compensatory demographic factors.

  16. Complex of Escherichia coli primary replicative helicase DnaB protein with a replication fork: recognition and structure.

    PubMed

    Jezewska, M J; Rajendran, S; Bujalowski, W

    1998-03-01

    Interactions of the Escherichia coli replicative helicase DnaB protein, with DNA replication fork substrates, have been studied using rigorous fluorescence titration, fluorescence energy transfer, and analytical ultracentrifugation methods. DnaB binds the 5' single-arm fork, the 3' single-arm fork, and the two-arm fork with stoichiometries of 1, 1, and 2 DnaB hexamers per fork, independent of the length of the duplex part of the fork. Within the structurally heterogeneous binding site, the helicase accesses most of the 20 nucleotide residues of an arm. The dsDNA of the fork does not contribute to the affinity; however, it affects the positioning of the enzyme on the 5' or 3' arm. Fluorescence energy transfer experiments provide direct evidence that the DnaB helicase binds the 5' arm of the fork in a single orientation, with respect to the duplex part of the fork. The 33-kDa domains of the hexamer face the dsDNA, while the small 12-kDa domains face the 5' end of the arm. In the complex with the 3' arm, the helicase is bound in an opposite orientation when compared to the 5' arm. This is the first determination of the strict, single orientation of a helicase in the complex with a replication fork. The 3' arm accommodates a DnaB hexamer, while another hexamer is associated with the 5' arm. The complex of two DnaB hexamers bound in opposite orientations with each arm of the fork may play an important role during bidirectional replication of the E. coli DNA.

  17. Linking habitat quality with trophic performance of steelhead along forest gradients in the South Fork Trinity River Watershed, California

    USGS Publications Warehouse

    McCarthy, Sarah G.; Duda, Jeffrey J.; Emlen, John M.; Hodgson, Garth R.; Beauchamp, David A.

    2009-01-01

    We examined invertebrate prey, fish diet, and energy assimilation in relation to habitat variation for steelhead Oncorhynchus mykiss (anadromous rainbow trout) and rainbow trout in nine low-order tributaries of the South Fork Trinity River, northern California. These streams spanned a range of environmental conditions, which allowed us to use bioenergetics modeling to determine the relative effects of forest cover, stream temperature, season, and fish age on food consumption and growth efficiency. Evidence of seasonal shifts in reliance on aquatic versus terrestrial food sources was detected among forest cover categories and fish ages, although these categories were not robust indicators of O. mykiss condition and growth efficiency. Consumption estimates were generally less than 20% of maximum consumption, and fish lost weight in some streams during summer low-flow conditions when stream temperatures exceeded 15°C. Current 100-year climate change projections for California threaten to exacerbate negative growth patterns and may undermine the productivity of this steelhead population, which is currently not listed as endangered or threatened. To demonstrate the potential effect of global warming on fish growth, we ran three climate change scenarios in two representative streams. Simulated temperature increases ranging from 1.4°C to 5.5°C during the summer and from 1.5°C to 2.9C during the winter amplified the weight loss; estimated average growth for age-1 fish was 0.4–4.5 times lower than normal (low to high estimated temperature increase) in the warm stream and 0.05–0.8 times lower in the cool stream. We conclude that feeding rate and temperature during the summer currently limit the growth and productivity of steelhead and rainbow trout in low-order streams in the South Fork Trinity River basin and predict that climate change will have detrimental effects on steelhead growth as well as on macroinvertebrate communities and stream ecosystems in general.

  18. The Coefficient of the Voltage Induced Frequency Shift Measurement on a Quartz Tuning Fork

    PubMed Central

    Hou, Yubin; Lu, Qingyou

    2014-01-01

    We have measured the coefficient of the voltage induced frequency shift (VIFS) of a 32.768 KHz quartz tuning fork. Three vibration modes were studied: one prong oscillating, two prongs oscillating in the same direction, and two prongs oscillating in opposite directions. They all showed a parabolic dependence of the eigen-frequency shift on the bias voltage applied across the fork, due to the voltage-induced internal stress, which varies as the fork oscillates. The average coefficient of the VIFS effect is as low as several hundred nano-Hz per millivolt, implying that fast-response voltage-controlled oscillators and phase-locked loops with nano-Hz resolution can be built. PMID:25414971

  19. Nascent DNA Proteomics Reveals a Chromatin Remodeler Required for Topoisomerase I Loading at Replication Forks.

    PubMed

    Ribeyre, Cyril; Zellweger, Ralph; Chauvin, Maeva; Bec, Nicole; Larroque, Christian; Lopes, Massimo; Constantinou, Angelos

    2016-04-12

    During transcription and DNA replication, the DNA template is overwound ahead of RNA and DNA polymerases and relaxed by DNA topoisomerases. Inhibitors of topoisomerases are potent anti-cancer agents. Camptothecin traps topoisomerase I on DNA and exerts preferential cytotoxicity toward cancer cells by way of its interference with the progression of replication forks. Starting with an unbiased proteomic analysis, we find that the chromatin remodeling complex BAZ1B-SMARCA5 accumulates near replication forks in camptothecin-exposed cells. We report that BAZ1B associates with topoisomerase I and facilitates its access to replication forks. Single-molecule analyses of replication structures show that BAZ1B contributes to replication interference by camptothecin. A lack of BAZ1B confers increased cellular tolerance of camptothecin. These findings reveal BAZ1B as a key facilitator of topoisomerase I function during DNA replication that affects the response of cancer cells to topoisomerase I inhibitors.

  20. Ubiquitinated Fancd2 recruits Fan1 to stalled replication forks to prevent genome instability.

    PubMed

    Lachaud, Christophe; Moreno, Alberto; Marchesi, Francesco; Toth, Rachel; Blow, J Julian; Rouse, John

    2016-02-19

    Mono-ubiquitination of Fancd2 is essential for repairing DNA interstrand cross-links (ICLs), but the underlying mechanisms are unclear. The Fan1 nuclease, also required for ICL repair, is recruited to ICLs by ubiquitinated (Ub) Fancd2. This could in principle explain how Ub-Fancd2 promotes ICL repair, but we show that recruitment of Fan1 by Ub-Fancd2 is dispensable for ICL repair. Instead, Fan1 recruitment--and activity--restrains DNA replication fork progression and prevents chromosome abnormalities from occurring when DNA replication forks stall, even in the absence of ICLs. Accordingly, Fan1 nuclease-defective knockin mice are cancer-prone. Moreover, we show that a Fan1 variant in high-risk pancreatic cancers abolishes recruitment by Ub-Fancd2 and causes genetic instability without affecting ICL repair. Therefore, Fan1 recruitment enables processing of stalled forks that is essential for genome stability and health.

  1. Maps showing mines, quarries, prospects, and exposures in the Devils Fork Roadless Area, Scott County, Virginia

    USGS Publications Warehouse

    Behum, Paul T.

    1984-01-01

    The Devils Fork Roadless Area is located at the eastern edge of the Appalachian coal region and is within the Cumberland Mountain section of the Appalachian Plateau physiographic province. Most of the area is drained by Devil Fork and its tributaries. Clinch Rock Branch of Straight Creek, Roddy Branch of Valley Creek, and Stinking Creek, all tributary to the Clinch River, drain small fringe tracts. Altitudes range from about 1,550 ft on the lower part of Straight Fork to about 3,490 ft at Cox Place on Little Mountain. Vegetation varies from mixed hardwoods in the uplands to thickets of conifer, rhododendron, and laurel in moist protected areas, as in coves along drainage courses.

  2. Pathological Changes of von Economo Neuron and Fork Neuron in Neuropsychiatric Diseases.

    PubMed

    Liu, Jia; Wang, Lu-ning; Arzberger, Thomas; Zhu, Ming-wei

    2016-02-01

    von Economo neuron (VEN) is a bipolar neuron characterized by a large spindle-shaped soma. VEN is generally distributed in the layer V of anterior insular lobe and anterior cingulate cortex. Fork neuron is another featured bipolar neuron. In recent years,many studies have illustrated that VEN and fork neurons are correlated with complicated cognition such as self-consciousness and social emotion. Studies in the development and morpholigies of these two neurons as well as their pathological changes in various neurological and psychiatric disorders have found that the abnormal number and functions of VEN can cause corresponding dysfunctions in social recognition and emotions both during the neuro-developmental stages of childhood and during the nerve degeneration in old age stage. Therefore, more attentions should be paid on the research of VEN and fork neurons in neuropsychiatric diseases.

  3. Ubiquitinated Fancd2 recruits Fan1 to stalled replication forks to prevent genome instability.

    PubMed

    Lachaud, Christophe; Moreno, Alberto; Marchesi, Francesco; Toth, Rachel; Blow, J Julian; Rouse, John

    2016-02-19

    Mono-ubiquitination of Fancd2 is essential for repairing DNA interstrand cross-links (ICLs), but the underlying mechanisms are unclear. The Fan1 nuclease, also required for ICL repair, is recruited to ICLs by ubiquitinated (Ub) Fancd2. This could in principle explain how Ub-Fancd2 promotes ICL repair, but we show that recruitment of Fan1 by Ub-Fancd2 is dispensable for ICL repair. Instead, Fan1 recruitment--and activity--restrains DNA replication fork progression and prevents chromosome abnormalities from occurring when DNA replication forks stall, even in the absence of ICLs. Accordingly, Fan1 nuclease-defective knockin mice are cancer-prone. Moreover, we show that a Fan1 variant in high-risk pancreatic cancers abolishes recruitment by Ub-Fancd2 and causes genetic instability without affecting ICL repair. Therefore, Fan1 recruitment enables processing of stalled forks that is essential for genome stability and health. PMID:26797144

  4. Design of a new portable fork detector for research reactor spent fuel

    SciTech Connect

    Hsue, S.T.; Menlove, H.O.; Rinard, P.M.

    1995-02-01

    There are many situations in nonproliferation and international safeguards when one needs to verify spent research-reactor fuel. Special inspections, a reactor coming under safeguards for the first time, and failed surveillance are prime examples. Several years ago, Los Alamos developed the FORK detector for the IAEA and EURATOM. This detector, together with the GRAND electronics package, is used routinely by inspectors to verify light-water-reactor spent fuels. Both the FORK detector and the GRAND electronics technologies have been transferred and are now commercially available. Recent incidents in the world indicate that research-reactor fuel is potentially a greater concern for proliferation than light-water-reactor fuels. A device similar to the FORK/GRAND should be developed to verify research-reactor spent fuels because the signals from light-water-reactor spent fuel are quite different than those from research-reactor fuels.

  5. Salivary gland determination in Drosophila: a salivary-specific, fork head enhancer integrates spatial pattern and allows fork head autoregulation.

    PubMed

    Zhou, B; Bagri, A; Beckendorf, S K

    2001-09-01

    In the early Drosophila embryo, a system of coordinates is laid down by segmentation genes and dorsoventral patterning genes. Subsequently, these coordinates must be interpreted to define particular tissues and organs. To begin understanding this process for a single organ, we have studied how one of the first salivary gland genes, fork head (fkh), is turned on in the primordium of this organ, the salivary placode. A placode-specific fkh enhancer was identified 10 kb from the coding sequence. Dissection of this enhancer showed that the apparently homogeneous placode is actually composed of at least four overlapping domains. These domains appear to be developmentally important because they predict the order of salivary invagination, are evolutionarily conserved, and are regulated by patterning genes that are important for salivary development. Three dorsoventral domains are defined by EGF receptor (EGFR) signaling, while stripes located at the anterior and posterior edges of the placode depend on wingless signaling. Further analysis identified sites in the enhancer that respond either positively to the primary activator of salivary gland genes, SEX COMBS REDUCED (SCR), or negatively to EGFR signaling. These results show that fkh integrates spatial pattern directly, without reference to other early salivary gland genes. In addition, we identified a binding site for FKH protein that appears to act in fkh autoregulation, keeping the gene active after SCR has disappeared from the placode. This autoregulation may explain how the salivary gland maintains its identity after the organ is established. Although the fkh enhancer integrates information needed to define the salivary placode, and although fkh mutants have the most extreme effects on salivary gland development thus far described, we argue that fkh is not a selector gene for salivary gland development and that there is no master, salivary gland selector gene. Instead, several genes independently sense spatial

  6. 77 FR 66865 - Record of Decision for the Oil and Gas Management Plan, Big South Fork National River and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... National Park Service Record of Decision for the Oil and Gas Management Plan, Big South Fork National River... (ROD) for the Oil and Gas Management Plan (Plan) for Big South Fork National River and Recreation Area... impacts from oil and gas development. The plan will guide oil and gas management in Big South...

  7. 77 FR 64125 - Notice of Proposed Withdrawal Extension and Notification of a Public Meeting for the East Fork...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-18

    ... (58 FR 16628 (1993)), as corrected by PLO No. 6980 (58 FR 33025 (1993)), will expire on March 29, 2013... for the East Fork Elk Winter Range; WY AGENCY: Bureau of Land Management, Interior. ACTION: Notice... States mining laws, to protect the East Fork Elk Winter Range and elk natural feeding grounds in...

  8. 75 FR 28673 - Carolina Coastal Railway, Inc.-Acquisition and Operation Exemption-Morehead & South Fork Railroad...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-21

    ... Federal Register on January 15, 2010 (75 FR 2580), titled ``Carolina Coastal Railway, Inc.--Acquisition... & South Fork Railroad Co. AGENCY: Surface Transportation Board. ACTION: Correction to Notice of... acquire, by assignment, the lease of Morehead & South Fork Railroad Co., Inc. (MHSF) with North...

  9. 76 FR 35009 - Draft Oil and Gas Management Plan/Environmental Impact Statement for Big South Fork National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... and gas management plan for BISO and OBRI was published in the Federal Register on May 31, 2006 (71 FR... National Park Service Draft Oil and Gas Management Plan/Environmental Impact Statement for Big South Fork... Big South Fork National River and Recreation Area and Obed Wild and Scenic River. SUMMARY: Pursuant...

  10. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  11. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  12. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  13. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  14. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River,...

  15. Integrating Salmon Recovery, Clean Water Act Compliance, Restoration, and Climate Change Impacts in the South Fork Nooksack River

    EPA Science Inventory

    "The South Fork Nooksack River (SFNR) is an important tributary to the Nooksack River, Bellingham Bay, and the Salish Sea. The South Fork Nooksack River comprises one of the 22 independent populations of spring Chinook in the Puget Sound Chinook Evolutionarily Significant Un...

  16. Measurements of Vortex Line Density Generated by a Quartz Tuning Fork in Superfluid 4He

    NASA Astrophysics Data System (ADS)

    Jackson, M. J.; Kolosov, O.; Schmoranzer, D.; Skrbek, L.; Tsepelin, V.; Woods, A. J.

    2016-05-01

    We present proof-of-concept measurements of the vortex line density generated by a quartz tuning fork resonator probed by the attenuation of second sound in superfluid ^4He at 1.6 K. The force-velocity response of a quartz tuning fork operating at a frequency of 31 kHz exhibited the onset of extra damping at a velocity of 0.5 ms^{-1}. Attenuation of the 5th resonant mode of second sound was observed at the same velocity, indicating the production of vortex lines. Our measurements demonstrate that an increase of the drag coefficient corresponds to the development of quantum turbulence.

  17. Far field diffraction of an optical vortex beam by a fork-shaped grating

    NASA Astrophysics Data System (ADS)

    Stoyanov, Lyubomir; Topuzoski, Suzana; Stefanov, Ivan; Janicijevic, Ljiljana; Dreischuh, Alexander

    2015-09-01

    In this work we report experimental data confirming the analytically predicted transformation of the topological charge (TC) of an input optical vortex (OV) beam, generated by means of fork-shaped binary computer-generated hologram (CGH), after a second fork-shaped binary CGH. The final TC of the vortex is confirmed to be equal to the TC of the incident beam plus the diffraction order (with its sign) times the TC encoded in the binary grating. The radii of the transformed OVs in the far field also are found to agree fairly well with these predicted by the analytical theory.

  18. Water-quality data for streams in the Upper North Fork of the Gunnison River, Colorado

    USGS Publications Warehouse

    Norris, J.M.; Maura, W.S.

    1985-01-01

    The upper reaches of the North Fork of the Gunnison River have been an area of active coal mining for many years. Recently, concerns about impacts of coal mining on surface-water quality have been raised. To answer these concerns, information on existing, or background, water quality must be known. To obtain this information for the study area, a program for the synoptic collection of water quality data was established in 1982. Water quality data were collected on continuously flowing streams in the upper North Fork of the Gunnison River basin in 1982 and 1983. Each site was sampled repetitively as changes occurred in streamflow and specific conductance. (USGS)

  19. Burnup verification tests with the FORK measurement system-implementation for burnup credit

    SciTech Connect

    Ewing, R.I.

    1994-08-01

    Verification measurements may be used to help ensure nuclear criticality safety when burnup credit is applied to spent fuel transport and storage systems. The FORK system measures the passive neutron and gamma-ray emission from spent fuel assemblies while in the storage pool. It was designed at Los Alamos National Laboratory for the International Atomic Energy Agency safeguards program and is well suited to verify burnup and cooling time records at commercial Pressurized Water Reactor (PWR) sites. This report deals with the application of the FORK system to burnup credit operations.

  20. Regulating telomere length from the inside out: the replication fork model

    PubMed Central

    2016-01-01

    Telomere length is regulated around an equilibrium set point. Telomeres shorten during replication and are lengthened by telomerase. Disruption of the length equilibrium leads to disease; thus, it is important to understand the mechanisms that regulate length at the molecular level. The prevailing protein-counting model for regulating telomerase access to elongate the telomere does not explain accumulating evidence of a role of DNA replication in telomere length regulation. Here I present an alternative model: the replication fork model that can explain how passage of a replication fork and regulation of origin firing affect telomere length. PMID:27401551

  1. Delayed complete gastric outlet obstruction due to a dinner fork: report of a case.

    PubMed

    Atila, Koray; Unek, Tarkan; Sevinç, Ali Ibrahim; Aydoğan, Baki; Serin, Ayfer; Bora, Seymen; Gülay, Hüseyin

    2010-07-01

    Ingestion of foreign bodies can be a common problem especially among children, alcoholics, psychiatric patients, and senile patients, but ingestion of a metallic dinner fork is uncommon. Foreign bodies with smooth edges usually do not pose significant problems, but a sharp foreign object that is not retrieved at the earliest may penetrate the wall and cause complications. Ingested foreign bodies usually pass the intestinal tract without problems, and perforation occurs in less than 1%. In this paper, a case of rare gastric outlet obstruction due to a dinner fork, which was ingested 25 days before, is reported. PMID:20849060

  2. Stimulated Raman rotational photoacoustic spectroscopy using a quartz tuning fork and femtosecond excitation

    NASA Astrophysics Data System (ADS)

    Schippers, W.; Gershnabel, E.; Burgmeier, J.; Katz, O.; Willer, U.; Averbukh, I. S.; Silberberg, Y.; Schade, W.

    2011-11-01

    Molecular alignment of linear molecules (O2, N2, CO2 and CO) is measured photoacoustically in the gas phase. The rotational excitation is accomplished using a simple femtosecond stimulated Raman excitation scheme, employing two femtosecond pulses with variable delay between the pulses. Molecular alignment is determined directly by measuring the energy dumped into the gas by quartz-enhanced photoacoustic spectroscopy (QEPAS), utilizing a quartz tuning fork as a sensitive photoacoustic transducer. The experimental results demonstrate for the first time the use of a tuning fork for resonant photoacoustic detection of Raman spectra excited by femtosecond double pulses and match both simulation and literature values.

  3. Fluvial sediment in Salem Fork watershed, West Virginia

    USGS Publications Warehouse

    Flint, R.F.

    1972-01-01

    Suspended sediment discharged from the 8.32-square-mile Salem Fork study area in Harrison County, W. Va., averaged 3,500 tons per year during the first 4-year period of investigation and 1,770 tons per year during the second 4-year period. The difference as attributed to increased flow control, effected by the completion of detention structures and other conservation measures, the absence of appreciable sediment-producing construction activities, and a reduction of the amounts of rainfall and runoff during the second 4-year period. Particle-size distribution of the suspended sediment discharged from the watershed remained unchanged during the two 4-year periods. Although sand and some silt were deposited in upstream reservoirs, sands and other sediments were evidently entrained in the flow below the reservoirs. During the 7.75-year period, reservoir 11A had a trap efficiency of 88 percent. The average annual sediment yield of subwatershed 11A was 1.31 tons per acre, or 837 tons per square mile. Outflow from reservoir 11A occurred during 81 percent of the investigation 'period, October 1954 to June 1962, and 78 percent of the sediment discharge from the reservoir occurred during less than 6 percent of the investigation period. A comparison of particle-size distribution of inflow sediment with that of outflow sediment revealed that practically all sands and some silts entering reservoir 11A were deposited in the reservoir. Chemical analyses of inflow water and the particle-size analyses suggested that flocculation of fine sediments occurred in the reservoir. Analysis of the sediment data collected at the outflow of reservoir 9 during 1956-62 revealed that the average annual sediment discharge was 128,000 pounds per year. Limited particle-size data suggested that practically no sand was discharged from reservoir 9, even though the inflow contained sand. Average annual inflow to reservoirs 11A and 9 compared favorably with average annual runoff for the entire watershed

  4. EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair.

    PubMed

    Wu, Yuehan; Lee, Suk-Hee; Williamson, Elizabeth A; Reinert, Brian L; Cho, Ju Hwan; Xia, Fen; Jaiswal, Aruna Shanker; Srinivasan, Gayathri; Patel, Bhavita; Brantley, Alexis; Zhou, Daohong; Shao, Lijian; Pathak, Rupak; Hauer-Jensen, Martin; Singh, Sudha; Kong, Kimi; Wu, Xaiohua; Kim, Hyun-Suk; Beissbarth, Timothy; Gaedcke, Jochen; Burma, Sandeep; Nickoloff, Jac A; Hromas, Robert A

    2015-12-01

    Replication fork stalling and collapse is a major source of genome instability leading to neoplastic transformation or cell death. Such stressed replication forks can be conservatively repaired and restarted using homologous recombination (HR) or non-conservatively repaired using micro-homology mediated end joining (MMEJ). HR repair of stressed forks is initiated by 5' end resection near the fork junction, which permits 3' single strand invasion of a homologous template for fork restart. This 5' end resection also prevents classical non-homologous end-joining (cNHEJ), a competing pathway for DNA double-strand break (DSB) repair. Unopposed NHEJ can cause genome instability during replication stress by abnormally fusing free double strand ends that occur as unstable replication fork repair intermediates. We show here that the previously uncharacterized Exonuclease/Endonuclease/Phosphatase Domain-1 (EEPD1) protein is required for initiating repair and restart of stalled forks. EEPD1 is recruited to stalled forks, enhances 5' DNA end resection, and promotes restart of stalled forks. Interestingly, EEPD1 directs DSB repair away from cNHEJ, and also away from MMEJ, which requires limited end resection for initiation. EEPD1 is also required for proper ATR and CHK1 phosphorylation, and formation of gamma-H2AX, RAD51 and phospho-RPA32 foci. Consistent with a direct role in stalled replication fork cleavage, EEPD1 is a 5' overhang nuclease in an obligate complex with the end resection nuclease Exo1 and BLM. EEPD1 depletion causes nuclear and cytogenetic defects, which are made worse by replication stress. Depleting 53BP1, which slows cNHEJ, fully rescues the nuclear and cytogenetic abnormalities seen with EEPD1 depletion. These data demonstrate that genome stability during replication stress is maintained by EEPD1, which initiates HR and inhibits cNHEJ and MMEJ.

  5. EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair

    PubMed Central

    Wu, Yuehan; Lee, Suk-Hee; Williamson, Elizabeth A.; Reinert, Brian L.; Cho, Ju Hwan; Xia, Fen; Jaiswal, Aruna Shanker; Srinivasan, Gayathri; Patel, Bhavita; Brantley, Alexis; Zhou, Daohong; Shao, Lijian; Pathak, Rupak; Hauer-Jensen, Martin; Singh, Sudha; Kong, Kimi; Wu, Xaiohua; Kim, Hyun-Suk; Beissbarth, Timothy; Gaedcke, Jochen; Burma, Sandeep; Nickoloff, Jac A.; Hromas, Robert A.

    2015-01-01

    Replication fork stalling and collapse is a major source of genome instability leading to neoplastic transformation or cell death. Such stressed replication forks can be conservatively repaired and restarted using homologous recombination (HR) or non-conservatively repaired using micro-homology mediated end joining (MMEJ). HR repair of stressed forks is initiated by 5’ end resection near the fork junction, which permits 3’ single strand invasion of a homologous template for fork restart. This 5’ end resection also prevents classical non-homologous end-joining (cNHEJ), a competing pathway for DNA double-strand break (DSB) repair. Unopposed NHEJ can cause genome instability during replication stress by abnormally fusing free double strand ends that occur as unstable replication fork repair intermediates. We show here that the previously uncharacterized Exonuclease/Endonuclease/Phosphatase Domain-1 (EEPD1) protein is required for initiating repair and restart of stalled forks. EEPD1 is recruited to stalled forks, enhances 5’ DNA end resection, and promotes restart of stalled forks. Interestingly, EEPD1 directs DSB repair away from cNHEJ, and also away from MMEJ, which requires limited end resection for initiation. EEPD1 is also required for proper ATR and CHK1 phosphorylation, and formation of gamma-H2AX, RAD51 and phospho-RPA32 foci. Consistent with a direct role in stalled replication fork cleavage, EEPD1 is a 5’ overhang nuclease in an obligate complex with the end resection nuclease Exo1 and BLM. EEPD1 depletion causes nuclear and cytogenetic defects, which are made worse by replication stress. Depleting 53BP1, which slows cNHEJ, fully rescues the nuclear and cytogenetic abnormalities seen with EEPD1 depletion. These data demonstrate that genome stability during replication stress is maintained by EEPD1, which initiates HR and inhibits cNHEJ and MMEJ. PMID:26684013

  6. Drilling, construction, and aquifer-test data from wells 3-3307-20 and -21, Thompson Corner exploratory wells I and II, Oahu, Hawaii

    USGS Publications Warehouse

    Presley, T.K.; Oki, D.S.

    1996-01-01

    The Thompson Corner exploratory wells I and II (State well numbers 3-3307-20 and -21) were drilled near Thompson Corner, about 2.2 miles south-southwest of the town of Haleiwa. The wells are located on agricultural land in the Waialua ground-water area. The wells are about 50 feet apart and penetrate about 90 feet into the ground water. Aquifer tests were conducted using well 3-3307-20 as a pumping well and well 3-3307-21 as an observation well. Well-construction data, logs of drilling notes, geologic descriptions for the samples, and aquifer-test data are presented for the wells. The wells are two of twelve exploratory wells drilled in the north-central Oahu area between July 1993 and May 1994 in cooperation with the Honolulu Board of Water Supply.

  7. Channelization and floodplain forests: Impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

    USGS Publications Warehouse

    Oswalt, S.N.; King, S.L.

    2005-01-01

    We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased overbank flooding, a rise in the groundwater table, and ponding of upstream timber. Our objectives were to: (1) determine the composition of floodplain vegetation communities along the degraded river reach, (2) to isolate relationships among these communities, geomorphic features, and environmental variables and (3) evaluate successional changes based on current stand conditions. Vegetation communities were not specifically associated with predefined geomorphic features; nevertheless, hydrologic and geomorphic processes as a result of channelization have clearly affected vegetation communities. The presence of valley plugs and continued degradation of upstream reaches and tributaries on the impacted study reach has arrested recovery of floodplain plant communities. Historically common species like Liquidambar styraciflua L. and Quercus spp. L. were not important, with importance values (IV) less than 1, and occurred in less than 20% of forested plots, while Acer rubrum L., a disturbance-tolerant species, was the most important species on the site (IV = 78.1) and occurred in 87% of forested plots. The results of this study also indicate that channelization impacts on the Middle Fork Forked Deer River are more temporally and spatially complex than previously described for other river systems. Rehabilitation of this system necessitates a long-term, landscape-scale solution that addresses watershed rehabilitation in a spatially and temporally hierarchical manner. ?? 2005 Elsevier B.V. All rights reserved.

  8. A cost-benefit analysis of preventative management for zebra and quagga mussels in the Colorado-Big Thompson System

    USGS Publications Warehouse

    Thomas, Catherine M.

    2010-01-01

    net benefits of preventative management strategies. This study builds a bioeconomic simulation model to predict and compare the expected economic costs of the CDOW boat inspection program ot the benefits of reduced expected control costs to water conveyance systems, hydropower generation stations, and minicipal water treatment facilities. The model is based on a case study water delivery and storage system, the Colorado-Big Thompson system. The Colorado-Big Thomspon system is an excellent example of water systems in the Rocky Mountain West. The system is nearly entirely man-made, with all of its reservoirs and delivery points connected via pipelines, tunnels, and canals. The structures and hydropower systems of the Colorado-Big Thompson system are common to other western storage and delivery systems, making the methods and insight developed from this case study transferal to other western systems. The model developed in this study contributes to the bioeconomic literature in several ways. Foremost, the model predicts the spread of dreissena mussels and associated damage costs for a connected water system in the Rocky Mountain West. Very few zebra mussel studies have focused on western water systems. Another distinguishing factor is the simultaneous consideration of spread from propagules introduced by boats and by flows. Most zebra mussel dispersal models consider boater movement patterns combined with limnological characteristics as predictors of spread. A separate set of studies have addressed mussel spread via downstream flows. To the author's knowledge, this is the first study that builds a zebra mussel spread model that specifically accounts for propagule pressure from boat introductions and from downstream flow introductions. By modeling an entire connected system, the study highlights how the spatial layout of a system, and the risk of invasion within a system affect the benefits of preventative management. This report is presented in five chapters. The first

  9. A celebration of mechanics: from nano to macro. The J. Michael T. Thompson Festschrift issue

    PubMed Central

    Elishakoff, Isaac

    2013-01-01

    This Theme Issue is dedicated to the topic ‘Mechanics: from nano to macro’ and marks the 75th birthday of Dr J. Michael T. Thompson, Fellow of the Royal Society, whose current affiliations are as follows: (i) Honorary Fellow, Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge; (ii) Emeritus Professor of Nonlinear Dynamics, Department of Civil, Environmental and Geomatic Engineering, University College London; and (iii) Professor of Theoretical and Applied Dynamics (Distinguished Sixth Century Chair, part-time), University of Aberdeen. He also serves as Chairman of the Board of Directors at ES-Consult (consulting engineers) in Copenhagen, Denmark. The pertinent question that arises from the very start is: should we first salute Michael and then describe the Theme Issue, or vice versa? Indeed, according to Blaise Pascal (1623–1662), the last thing one discovers in composing a work is what to put first. I would like to take the liberty of deviating from the tradition of the Philosophical Transactions and start with the tribute to Michael; after all he is the prime cause of this Theme Issue. PMID:23690647

  10. Hamiltonian Systems and Optimal Control in Computational Anatomy: 100 Years Since D'Arcy Thompson.

    PubMed

    Miller, Michael I; Trouvé, Alain; Younes, Laurent

    2015-01-01

    The Computational Anatomy project is the morphome-scale study of shape and form, which we model as an orbit under diffeomorphic group action. Metric comparison calculates the geodesic length of the diffeomorphic flow connecting one form to another. Geodesic connection provides a positioning system for coordinatizing the forms and positioning their associated functional information. This article reviews progress since the Euler-Lagrange characterization of the geodesics a decade ago. Geodesic positioning is posed as a series of problems in Hamiltonian control, which emphasize the key reduction from the Eulerian momentum with dimension of the flow of the group, to the parametric coordinates appropriate to the dimension of the submanifolds being positioned. The Hamiltonian viewpoint provides important extensions of the core setting to new, object-informed positioning systems. Several submanifold mapping problems are discussed as they apply to metamorphosis, multiple shape spaces, and longitudinal time series studies of growth and atrophy via shape splines.

  11. Acoustic Doppler current profiling from the JGOFS Arabian Sea cruises aboard the RV T.G. THOMPSON: TN043, January 8, 1995--February 4, 1995; TN044, February 8, 1995--February 25, 1995; TN045, March 14, 1995--April 10, 1995; TN046, April 14, 1995--April 29, 1995

    SciTech Connect

    Flagg, C.N.; Kim, H.S.; Shi, Y.

    1995-09-01

    Acoustic Doppler current profiler (ADCP) data from the R/V T.G. THOMPSON is part of the core data for the US JGOFS Arabian Sea project along with hydrographic and nutrient data. Seventeen cruises on the THOMPSON are scheduled to take place between September 1994 and January 1996. This is the second in a series of data reports covering the ADCP data from the Arabian Sea JGOFS cruises TNO43 through TNO46. ADCP data are being collected on all the JGOFS Arabian Sea cruises using an autonomous data acquisition system developed for ship-of-opportunity cruises. This system, referred to as the AutoADCP, makes it possible to collect the ADCP data without the constant monitoring usually necessary and assures constant data coverage and uniform data quality. This data report presents ADCP results from the second group of four JGOFS cruises, TNO43 through TNO46, concentrating on the data collection and processing methods. The ADCP data itself reside in a CODAS data base at Brookhaven National Laboratory and is generally available to JGOFS investigators through contact with the authors. The CODAS data base and associated ADCP processing software were developed over a number of years by Eric Firing and his group at the University of Hawaii. The CODAS software is shareware available for PC`s or Unix computers and is the single most widely used ADCP processing program for ship mounted units.

  12. Infrared Observations of Mars South Polar Residual Cap: When Eating Swiss Cheese - Use a Fork

    NASA Astrophysics Data System (ADS)

    Titus, T. N.; Brown, A. J.; Seelos, F. P.; Murchie, S. L.; Piqueux, S.; Christensen, P. R.; CRISM Team

    2008-03-01

    On the edge of the Mars southern residual cap is a region known as the fork region. This region contains scarps, CO2 Swiss cheese mesas, and a strip of exposed H2O ice 10 km wide. We examine this region using both THEMIS and CRISM observations.

  13. Formation of a DNA loop at the replication fork generated by bacteriophage T7 replication proteins.

    PubMed

    Park, K; Debyser, Z; Tabor, S; Richardson, C C; Griffith, J D

    1998-02-27

    Intermediates in the replication of circular and linear M13 double-stranded DNA by bacteriophage T7 proteins have been examined by electron microscopy. Synthesis generated double-stranded DNA molecules containing a single replication fork with a linear duplex tail. A complex presumably consisting of T7 DNA polymerase and gene 4 helicase/primase molecules was present at the fork together with a variable amount of single-stranded DNA sequestered by gene 2.5 single-stranded DNA binding protein. Analysis of the length distribution of Okazaki fragments formed at different helicase/primase concentrations was consistent with coupling of leading and lagging strand replication. Fifteen to forty percent of the templates engaged in replication have a DNA loop at the replication fork. The loops are fully double-stranded with an average length of approximately 1 kilobase. Labeling with biotinylated dCTP showed that the loops consist of newly synthesized DNA, and synchronization experiments using a linear template with a G-less cassette demonstrated that the loops are formed by active displacement of the lagging strand. A long standing feature of models for coupled leading/lagging strand replication has been the presence of a DNA loop at the replication fork. This study provides the first direct demonstration of such loops.

  14. 78 FR 74005 - Amendment of Class D and Class E Airspace; Grand Forks, ND

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The incorporation by reference in 14... Federal Aviation Administration 14 CFR Part 71 Amendment of Class D and Class E Airspace; Grand Forks,...

  15. 75 FR 47621 - Final Environmental Impact Statement for the North Fork Rancheria's Proposed 305-Acre Trust...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ... proposed action in the Federal Register on October 27, 2004 (69 FR 62721). The BIA held a public scoping... Availability for the Draft EIS (DEIS) was published in the Federal Register on February 15, 2008 (73 FR 8898... Bureau of Indian Affairs Final Environmental Impact Statement for the North Fork Rancheria's Proposed...

  16. Stabilization and targeting of INO80 to replication forks by BAP1 during normal DNA synthesis.

    PubMed

    Lee, Han-Sae; Lee, Shin-Ai; Hur, Shin-Kyoung; Seo, Jeong-Wook; Kwon, Jongbum

    2014-10-06

    The INO80 chromatin-remodelling complex has been implicated in DNA replication during stress in yeast. However, its role in normal DNA replication and its underlying mechanisms remain unclear. Here, we show that INO80 binds to replication forks and promotes fork progression in human cells under unperturbed, normal conditions. We find that Ino80, which encodes the catalytic ATPase of INO80, is essential for mouse embryonic DNA replication and development. Ino80 is recruited to replication forks through interaction with ubiquitinated H2A--aided by BRCA1-associated protein-1 (BAP1), a tumour suppressor and nuclear de-ubiquitinating enzyme that also functions to stabilize Ino80. Importantly, Ino80 is downregulated in BAP1-defective cancer cells due to the lack of an Ino80 stabilization mechanism via BAP1. Our results establish a role for INO80 in normal DNA replication and uncover a mechanism by which this remodeler is targeted to replication forks, suggesting a molecular basis for the tumour-suppressing function of BAP1.

  17. 78 FR 33016 - Proposed Amendment of Class D Airspace; Grand Forks AFB, ND

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-03

    ... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of... read as follows: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class D Airspace; Grand Forks AFB,...

  18. Human HLTF mediates postreplication repair by its HIRAN domain-dependent replication fork remodelling

    SciTech Connect

    Achar, Yathish Jagadheesh; Balogh, David; Neculai, Dante; Juhasz, Szilvia; Morocz, Monika; Gali, Himabindu; Dhe-Paganon, Sirano; Venclovas, Česlovas; Haracska, Lajos

    2015-09-08

    Defects in the ability to respond properly to an unrepaired DNA lesion blocking replication promote genomic instability and cancer. Human HLTF, implicated in error-free replication of damaged DNA and tumour suppression, exhibits a HIRAN domain, a RING domain, and a SWI/SNF domain facilitating DNA-binding, PCNA-polyubiquitin-ligase, and dsDNA-translocase activities, respectively. Here, we investigate the mechanism of HLTF action with emphasis on its HIRAN domain. We found that in cells HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Our biochemical assays show that HIRAN domain mutant HLTF proteins retain their ubiquitin ligase, ATPase and dsDNA translocase activities but are impaired in binding to a model replication fork. These data and our structural study indicate that the HIRAN domain recruits HLTF to a stalled replication fork, and it also provides the direction for the movement of the dsDNA translocase motor domain for fork reversal. We suggest functional similarities between the HIRAN, the OB, the HARP2, and other domains found in certain motor proteins, which may explain why only a subset of DNA translocases can carry out fork reversal.

  19. 77 FR 23059 - Endangered and Threatened Wildlife and Plants; Determination of Endangered Status for Three Forks...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-17

    ... Forks springsnail as a candidate for listing on October 30, 2001 (66 FR 54808). We first identified the San Bernardino springsnail as a candidate for listing on December 6, 2007 (72 FR 69034). Candidates... Bernardino springsnail. In our most recent annual Candidate Notice of Review dated November 10, 2010 (75...

  20. Inferring Causes of Biological Impairment in the Clear Fork Watershed, West Virginia (Final)

    EPA Science Inventory

    The Clear Fork, WV case study illustrates a causal assessment in a watershed using the USEPA stressor identification process described on www.epa.gov/caddis. This case study demonstrates that a watershed-wide causal assessment has several advantages for making analysis practical,...