Sample records for freezers

  1. 16 CFR Appendix A5 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service A5 Appendix A5 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service...

  2. 16 CFR Appendix A7 to Part 305 - Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service A7 Appendix A7 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service Range...

  3. 16 CFR Appendix A4 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service A4 Appendix A4 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service...

  4. 16 CFR Appendix A6 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service A6 Appendix A6 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service...

  5. 16 CFR Appendix A4 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service A4 Appendix A4 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service...

  6. 16 CFR Appendix A5 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service A5 Appendix A5 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service...

  7. 16 CFR Appendix A6 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service A6 Appendix A6 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service...

  8. 16 CFR Appendix A7 to Part 305 - Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service A7 Appendix A7 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service Range...

  9. 16 CFR Appendix A5 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service A5 Appendix A5 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service...

  10. 16 CFR Appendix A6 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service A6 Appendix A6 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service...

  11. 16 CFR Appendix A8 to Part 305 - Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service A8 Appendix A8 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service Range...

  12. 16 CFR Appendix A8 to Part 305 - Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service A8 Appendix A8 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service Range...

  13. 16 CFR Appendix A4 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service A4 Appendix A4 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service...

  14. 16 CFR Appendix A7 to Part 305 - Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service A7 Appendix A7 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service Range...

  15. 16 CFR Appendix A4 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service A4 Appendix A4 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service...

  16. 16 CFR Appendix A8 to Part 305 - Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service A8 Appendix A8 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service Range...

  17. 16 CFR Appendix A5 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service A5 Appendix A5 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service...

  18. 16 CFR Appendix A7 to Part 305 - Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service A7 Appendix A7 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service Range...

  19. 16 CFR Appendix A6 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service A6 Appendix A6 to Part 305 Commercial...—Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service...

  20. 16 CFR Appendix A8 to Part 305 - Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service A8 Appendix A8 to Part 305 Commercial...—Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service Range...

  1. 16 CFR Appendix A6 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Refrigerator-Freezers With Automatic Defrost With Bottom-Mounted Freezer Without Through-the-Door Ice Service A6 Appendix A6 to Part 305 Commercial... Freezer Without Through-the-Door Ice Service Range Information Manufacturer's Rated Total Refrigerated...

  2. 10 CFR 431.62 - Definitions concerning commercial refrigerators, freezers and refrigerator-freezers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...-section. Basic model means, with respect to commercial refrigerators, freezers, and refrigerator-freezers... 430); (2) Is not designed and marketed exclusively for medical, scientific, or research purposes; (3... standard product temperature-measuring device. Vertical Closed means equipment with hinged or sliding doors...

  3. 10 CFR 431.306 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., ceiling, and door insulation of at least R-25 for coolers and R-32 for freezers, except that this... insulation of at least R-28 for freezers; (5) For evaporator fan motors of under 1 horsepower and less than... freezers and windows in walk-in freezer doors shall be of triple-pane glass with either heat-reflective...

  4. 10 CFR 431.306 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., ceiling, and door insulation of at least R-25 for coolers and R-32 for freezers, except that this... insulation of at least R-28 for freezers; (5) For evaporator fan motors of under 1 horsepower and less than... freezers and windows in walk-in freezer doors shall be of triple-pane glass with either heat-reflective...

  5. 10 CFR 431.306 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., ceiling, and door insulation of at least R-25 for coolers and R-32 for freezers, except that this... insulation of at least R-28 for freezers; (5) For evaporator fan motors of under 1 horsepower and less than... freezers and windows in walk-in freezer doors shall be of triple-pane glass with either heat-reflective...

  6. 10 CFR 431.306 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., ceiling, and door insulation of at least R-25 for coolers and R-32 for freezers, except that this... insulation of at least R-28 for freezers; (5) For evaporator fan motors of under 1 horsepower and less than... freezers and windows in walk-in freezer doors shall be of triple-pane glass with either heat-reflective...

  7. 16 CFR Appendix A7 to Part 305 - Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Refrigerator-freezers With Automatic Defrost With Top-mounted Freezer With Through-the-door Ice Service A7 Appendix A7 to Part 305 Commercial... With Through-the-door Ice Service Range Information Manufacturer's Rated Total Refrigerated Volume in...

  8. 16 CFR Appendix A4 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Refrigerator-Freezers With Automatic Defrost With Top-Mounted Freezer Without Through-the-Door Ice Service A4 Appendix A4 to Part 305 Commercial... Without Through-the-Door Ice Service Range Information Manufacturer's Rated Total Refrigerated Volume in...

  9. 16 CFR Appendix A5 to Part 305 - Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Refrigerator-Freezers With Automatic Defrost With Side-Mounted Freezer Without Through-the-Door Ice Service A5 Appendix A5 to Part 305 Commercial... Without Through-the-Door Ice Service Range Information Manufacturer's Rated Total Refrigerated Volume in...

  10. 16 CFR Appendix A8 to Part 305 - Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Refrigerator-freezers With Automatic Defrost With Side-mounted Freezer With Through-the-door Ice Service A8 Appendix A8 to Part 305 Commercial... With Through-the-door Ice Service Range Information Manufacturer's Rated Total Refrigerated Volume in...

  11. Prevalence and associated features of self-reported freezing of gait in Parkinson disease: The DEEP FOG study.

    PubMed

    Amboni, M; Stocchi, F; Abbruzzese, G; Morgante, L; Onofrj, M; Ruggieri, S; Tinazzi, M; Zappia, M; Attar, M; Colombo, D; Simoni, L; Ori, A; Barone, P; Antonini, A

    2015-06-01

    Freezing of Gait (FOG) is a common and disabling symptom in patients with Parkinson disease (PD). The relationship between FOG and dopaminergic medication is complex. The aim of the present study was to estimate the prevalence of self-reported FOG, its associated clinical features, and its relationship with wearing-off in a wide PD population. This is an observational multicenter study of 634 consecutive non-demented PD patients. Patients were identified either as freezers or non-freezers based on item-3 of the Freezing of Gait-Questionnaire. FOG was then classified as on, off and onoff freezing based on its relationship with wearing-off. Patients were assessed with Unified Parkinson's Disease Rating Scale, Hoehn and Yahr scale, 8-item Parkinson's disease Questionnaire, Mini-Mental State Examination. Data from 593 patients were analyzed, 325 (54.3%) were freezers of whom 200 (61.6%) experienced FOG only during off state (off-freezers), 6 (1.8%) only during on state and 119 (36.6%) either in on and off states or independently of dopaminergic response-related symptoms (onoff-freezers). Overall, freezers vs non-freezers had longer disease duration, more advanced disease and greater disability. Moreover, freezers more frequently reported wearing-off and experienced worse quality of life. Onoff-freezers vs off-freezers were older, more severely disabled, less likely to experience wearing-off, treated with lower levodopa equivalent daily dose and with poorer cognitive performance. Self-reported FOG is mainly recognizable in advanced PD and is associated with more disability and worse quality of life. Onoff-FOG may represent the result of under-treatment or rather interpretable as a distinct clinical entity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. 10 CFR 431.62 - Definitions concerning commercial refrigerators, freezers and refrigerator-freezers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... functional characteristics that affect energy consumption. Commercial refrigerator, freezer, and refrigerator... formed by the plane of the door, when the equipment is viewed in cross-section; and (2) For equipment...

  13. NHEXAS PHASE I REGION 5 STUDY--STANDARD OPERATING PROCEDURE--REFRIGERATORS AND FREEZERS (NHX/SOP-163-001)

    EPA Science Inventory

    This procedure describes the calibration and maintenance activities of a refrigerator custodian in ensuring that refrigerators and freezers are functioning within acceptable temperature ranges. Refrigerators and freezers were used as temperature-controlled repositories for reagen...

  14. Altered effective connectivity contributes to micrographia in patients with Parkinson's disease and freezing of gait.

    PubMed

    Nackaerts, Evelien; Nieuwboer, Alice; Broeder, Sanne; Swinnen, Stephan; Vandenberghe, Wim; Heremans, Elke

    2018-02-01

    Recently, it was shown that patients with Parkinson's disease (PD) and freezing of gait (FOG) can also experience freezing episodes during handwriting and present writing problems outside these episodes. So far, the neural networks underlying increased handwriting problems in subjects with FOG are unclear. This study used dynamic causal modeling of fMRI data to investigate neural network dynamics underlying freezing-related handwriting problems and how these networks changed in response to visual cues. Twenty-seven non-freezers and ten freezers performed a pre-writing task with and without visual cues in the scanner with their right hand. The results showed that freezers and non-freezers were able to recruit networks involved in cued and uncued writing in a similar fashion. Whole group analysis also revealed a trend towards altered visuomotor integration in patients with FOG. Next, we controlled for differences in disease severity between both patient groups using a sensitivity analysis. For this, a subgroup of ten non-freezers matched for disease severity was selected by an independent researcher. This analysis further exposed significantly weaker coupling in mostly left hemispheric visuo-parietal, parietal - supplementary motor area, parietal - premotor, and premotor-M1 pathways in freezers compared to non-freezers, irrespective of cues. Correlation analyses revealed that these impairments in connectivity were related to writing amplitude and quality. Taken together, these findings show that freezers have reduced involvement of the supplementary motor area in the motor network, which explains the impaired writing amplitude regulation in this group. In addition, weaker supportive premotor connectivity may have contributed to micrographia in freezers, a pattern that was independent of cueing.

  15. The design and development of a vapor compression refrigerator/freezer for spacelab

    NASA Technical Reports Server (NTRS)

    Hye, A.

    1983-01-01

    A computer simulation was performed to determine the design criteria for a spacelab refrigerator/freezer using the test results of a vapor compression refrigerator/freezer which flew on STS-4 without problem. It has been established to have a vapor Reynolds number over 3000 at a vapor quality of 0.2 to maintain annular boiling in the evaporator and for the condenser to have a vapor Reynolds number over 15000 at its inlet to maintain annular condensation. These two constraints will virtually eliminate the effect of gravity on the performance of the refrigerator/freezer. These results are being used to build a refrigerator/freezer which will fly in Spacelab-4 scheduled for launch in December 1985.

  16. 75 FR 7520 - Certain Refrigerators and Components Thereof; Notice of the Commission's Final Determination of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-19

    ... claim construction of the terms ``freezer compartment,'' ``disposed within the freezer compartment... questions concerning claim construction. After receiving briefing from the parties, the Commission determined to modify the ALJ's claim constructions of the terms ``freezer compartment,'' ``disposed within...

  17. 76 FR 32142 - Bottom Mount Combination Refrigerator-Freezers From the Republic of Korea: Postponement of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-03

    ... DEPARTMENT OF COMMERCE International Trade Administration [C-580-866] Bottom Mount Combination... Department) initiated the countervailing duty investigation of bottom mount combination refrigerator-freezers from the Republic of Korea. See Bottom Mount Combination Refrigerator-Freezers From the Republic of...

  18. Domestic Refrigeration, Freezer, and Window Air Conditioner Service. Teacher Edition.

    ERIC Educational Resources Information Center

    Clemons, Mark

    This curriculum guide contains six units of instruction for a course in domestic refrigerator, freezer, and window air conditioner service. The units cover the following topics: (1) service fundamentals; (2) mechanical components and functions; (3) electrical components and control devices; (4) refrigerator and freezer service; (5) domestic ice…

  19. EVALUATION OF OZONE-FRIENDLY HYDROFLUOROPROPANE-BASED ZEOTROPIC REFRIGERANT MIXTURES IN A LORENZ-MEUTZNER REFRIGERATOR/FREEZER

    EPA Science Inventory

    The two-evaporator (located in the freezer and fresh food compartments) design of the Lorenz-Meutzner (L-M) refrigerator/freezer (R/F) makes it a leading candidate for use of zeotropic refrigerant mixtures. Zeotrophic mixtures can have significant temperature glides during evapor...

  20. Shuttle Kit Freezer Refrigeration Unit Conceptual Design

    NASA Technical Reports Server (NTRS)

    Copeland, R. J.

    1975-01-01

    The refrigerated food/medical sample storage compartment as a kit to the space shuttle orbiter is examined. To maintain the -10 F in the freezer kit, an active refrigeration unit is required, and an air cooled Stirling Cycle refrigerator was selected. The freezer kit contains two subsystems, the refrigeration unit, and the storage volume. The freezer must provide two basic capabilities in one unit. One requirement is to store 215 lbs of food which is consumed in a 30-day period by 7 people. The other requirement is to store 128.3 lbs of medical samples consisting of both urine and feces. The unit can be mounted on the lower deck of the shuttle cabin, and will occupy four standard payload module compartments on the forward bulkhead. The freezer contains four storage compartments.

  1. Virtual reality walking and dopamine: opening new doorways to understanding freezing of gait in Parkinson's disease.

    PubMed

    Matar, E; Shine, J M; Naismith, S L; Lewis, S J G

    2014-09-15

    Freezing of gait (FOG) is a disabling form of gait disturbance that is common in the advanced stages of Parkinson's disease (PD). Despite its prevalence, methods of studying and assessing FOG are limited. We have previously shown that a virtual reality paradigm was able to distinguish between those who report FOG ("freezers") and those who do not report FOG ("non-freezers"). In this paradigm, 'freezers' were found to have prolonged footstep latency in response to known triggers of FOG including doorways, sliding doors and dual-tasking. In this study, we employed the same paradigm to assess performance of 27 freezers and 14 non-freezers in their clinical 'on' and 'off' medication states. In this study, only participants in the freezing group demonstrated statistically significant increases in latencies experienced in the 'off' state compared to the 'on' state in response to wide and narrow doorways and the opening of a sliding door. By contrast, these behavioral differences were not apparent in non-freezers. Furthermore the delay was specific to environmental cues and was not due to generalized slowing in the 'off' state. The findings suggest that this motor delay when processing environmentally salient cues is specific to freezers and is partially mediated by dopamine-dependent neurocircuitry. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. Freezing of Gait in Parkinson's Disease: An Overload Problem?

    PubMed

    Beck, Eric N; Ehgoetz Martens, Kaylena A; Almeida, Quincy J

    2015-01-01

    Freezing of gait (FOG) is arguably the most severe symptom associated with Parkinson's disease (PD), and often occurs while performing dual tasks or approaching narrowed and cluttered spaces. While it is well known that visual cues alleviate FOG, it is not clear if this effect may be the result of cognitive or sensorimotor mechanisms. Nevertheless, the role of vision may be a critical link that might allow us to disentangle this question. Gaze behaviour has yet to be carefully investigated while freezers approach narrow spaces, thus the overall objective of this study was to explore the interaction between cognitive and sensory-perceptual influences on FOG. In experiment #1, if cognitive load is the underlying factor leading to FOG, then one might expect that a dual-task would elicit FOG episodes even in the presence of visual cues, since the load on attention would interfere with utilization of visual cues. Alternatively, if visual cues alleviate gait despite performance of a dual-task, then it may be more probable that sensory mechanisms are at play. In compliment to this, the aim of experiment#2 was to further challenge the sensory systems, by removing vision of the lower-limbs and thereby forcing participants to rely on other forms of sensory feedback rather than vision while walking toward the narrow space. Spatiotemporal aspects of gait, percentage of gaze fixation frequency and duration, as well as skin conductance levels were measured in freezers and non-freezers across both experiments. Results from experiment#1 indicated that although freezers and non-freezers both walked with worse gait while performing the dual-task, in freezers, gait was relieved by visual cues regardless of whether the cognitive demands of the dual-task were present. At baseline and while dual-tasking, freezers demonstrated a gaze behaviour that neglected the doorway and instead focused primarily on the pathway, a strategy that non-freezers adopted only when performing the dual-task. Interestingly, with the combination of visual cues and dual-task, freezers increased the frequency and duration of fixations toward the doorway, compared to non-freezers. These results suggest that although increasing demand on attention does significantly deteriorate gait in freezers, an increase in cognitive demand is not exclusively responsible for freezing (since visual cues were able to overcome any interference elicited by the dual-task). When vision of the lower limbs was removed in experiment#2, only the freezers' gait was affected. However, when visual cues were present, freezers' gait improved regardless of the dual-task. This gait behaviour was accompanied by greater amount of time spent looking at the visual cues irrespective of the dual-task. Since removing vision of the lower-limbs hindered gait even under low attentional demand, restricted sensory feedback may be an important factor to the mechanisms underlying FOG.

  3. How Does the Freezer Burn Our Food?

    ERIC Educational Resources Information Center

    Schmidt, Shelly J.; Lee, Joo Won

    2009-01-01

    Freezer burn is a common problem that significantly affects the color, texture, and flavor of frozen foods. Food science students should be able to clearly explain the causes and consequences of freezer burn. However, it is difficult to find a modern, detailed, accurate, yet concise, explanation of the mechanism and factors influencing the rate of…

  4. 76 FR 55044 - Bottom Mount Combination Refrigerator-Freezers From the Republic of Korea: Preliminary Negative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-06

    ... Mexico.\\6\\ The CVD investigation and the AD investigations have the same scope with regard to the... Mount Combination Refrigerator-Freezers From the Republic of Korea and Mexico: Initiation of Antidumping... Refrigerator-Freezers From Korea and Mexico, 76 FR 29791 (May 23, 2011); and USITC Publication 4232 entitled...

  5. 21 CFR 864.9700 - Blood storage refrigerator and blood storage freezer.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Blood storage refrigerator and blood storage... Establishments That Manufacture Blood and Blood Products § 864.9700 Blood storage refrigerator and blood storage freezer. (a) Identification. A blood storage refrigerator and a blood storage freezer are devices intended...

  6. 21 CFR 864.9700 - Blood storage refrigerator and blood storage freezer.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Blood storage refrigerator and blood storage... Establishments That Manufacture Blood and Blood Products § 864.9700 Blood storage refrigerator and blood storage freezer. (a) Identification. A blood storage refrigerator and a blood storage freezer are devices intended...

  7. 21 CFR 864.9700 - Blood storage refrigerator and blood storage freezer.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Blood storage refrigerator and blood storage... Establishments That Manufacture Blood and Blood Products § 864.9700 Blood storage refrigerator and blood storage freezer. (a) Identification. A blood storage refrigerator and a blood storage freezer are devices intended...

  8. 21 CFR 864.9700 - Blood storage refrigerator and blood storage freezer.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Blood storage refrigerator and blood storage... Establishments That Manufacture Blood and Blood Products § 864.9700 Blood storage refrigerator and blood storage freezer. (a) Identification. A blood storage refrigerator and a blood storage freezer are devices intended...

  9. 21 CFR 864.9700 - Blood storage refrigerator and blood storage freezer.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Blood storage refrigerator and blood storage... Establishments That Manufacture Blood and Blood Products § 864.9700 Blood storage refrigerator and blood storage freezer. (a) Identification. A blood storage refrigerator and a blood storage freezer are devices intended...

  10. 10 CFR 431.64 - Uniform test method for the measurement of energy consumption of commercial refrigerators...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... consumption of commercial refrigerators, freezers, and refrigerator-freezers. 431.64 Section 431.64 Energy... method for the measurement of energy consumption of commercial refrigerators, freezers, and refrigerator... energy consumption in kilowatt hours per day (kWh/day) for a given product category and volume or total...

  11. Experimental investigation of an alternating evaporator duty refrigerator/freezer

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lavanis, M.; Haider, I.; Radermacher, R.

    1998-12-31

    A bistable solenoid valve has been used to build an alternating evaporator duty (AED) domestic refrigerator/freezer. This refrigerator has two vapor compression refrigeration loops that share a common compressor, condenser, and suction line heat exchanger. Each of the refrigeration loops has an expansion device and evaporator. One evaporator is located in the fresh food compartment and the other is located in the freezer compartment. The bistable solenoid valve directs the flow of the refrigerant through one loop at a time. Only one of the two compartments is cooled at any given time. With this configuration, the food compartment is cooledmore » at a higher evaporator temperature than the freezer. Due to this, the energy efficiency of the refrigerator is improved by 8.5% over a conventional domestic refrigerator/freezer. Also, this cycle allows for completely independent temperature control of the freezer and fresh food compartments. There may be a penalty because this cycle does not allow for both loops to be simultaneously optimized. Isobutane was the only refrigerant used in this investigation.« less

  12. Does Anxiety Cause Freezing of Gait in Parkinson's Disease?

    PubMed Central

    Ehgoetz Martens, Kaylena A.; Ellard, Colin G.; Almeida, Quincy J.

    2014-01-01

    Individuals with Parkinson's disease (PD) commonly experience freezing of gait under time constraints, in narrow spaces, and in the dark. One commonality between these different situations is that they may all provoke anxiety, yet anxiety has never been directly examined as a cause of FOG. In this study, virtual reality was used to induce anxiety and evaluate whether it directly causes FOG. Fourteen patients with PD and freezing of gait (Freezers) and 17 PD without freezing of gait (Non-Freezers) were instructed to walk in two virtual environments: (i) across a plank that was located on the ground (LOW), (ii) across a plank above a deep pit (HIGH). Multiple synchronized motion capture cameras updated participants' movement through the virtual environment in real-time, while their gait was recorded. Anxiety levels were evaluated after each trial using self-assessment manikins. Freezers performed the experiment on two separate occasions (in their ON and OFF state). Freezers reported higher levels of anxiety compared to Non-Freezers (p<0.001) and all patients reported greater levels of anxiety when walking across the HIGH plank compared to the LOW (p<0.001). Freezers experienced significantly more freezing of gait episodes (p = 0.013) and spent a significantly greater percentage of each trial frozen (p = 0.005) when crossing the HIGH plank. This finding was even more pronounced when comparing Freezers in their OFF state. Freezers also had greater step length variability in the HIGH compared to the LOW condition, while the step length variability in Non-Freezers did not change. In conclusion, this was the first study to directly compare freezing of gait in anxious and non-anxious situations. These results present strong evidence that anxiety is an important mechanism underlying freezing of gait and supports the notion that the limbic system may have a profound contribution to freezing in PD. PMID:25250691

  13. Balance Asymmetry in Parkinson’s Disease and Its Contribution to Freezing of Gait

    PubMed Central

    Boonstra, Tjitske A.; van Vugt, Jeroen P. P.; van der Kooij, Herman; Bloem, Bastiaan R.

    2014-01-01

    Balance control (the ability to maintain an upright posture) is asymmetrically controlled in a proportion of patients with Parkinson’s disease. Gait asymmetries have been linked to the pathophysiology of freezing of gait. We speculate that asymmetries in balance could contribute to freezing by a) hampering the unloading of the stepping leg and/or b) leading to a preferred stance leg during gait, which then results in asymmetric gait. To investigate this, we examined the relationship between balance control and weight-bearing asymmetries and freezing. We included 20 human patients with Parkinson (tested OFF medication; nine freezers) and nine healthy controls. Balance was perturbed in the sagittal plane, using continuous multi-sine perturbations, applied by a motion platform and by a force at the sacrum. Applying closed-loop system identification techniques, relating the body sway angle to the joint torques of each leg separately, determined the relative contribution of each ankle and hip joint to the total amount of joint torque. We also calculated weight-bearing asymmetries. We determined the 99-percent confidence interval of weight-bearing and balance-control asymmetry using the responses of the healthy controls. Freezers did not have larger asymmetries in weight bearing (p = 0.85) nor more asymmetrical balance control compared to non-freezers (p = 0.25). The healthy linear one-to-one relationship between weight bearing and balance control was significantly different for freezers and non-freezers (p = 0.01). Specifically, non-freezers had a significant relationship between weight bearing and balance control (p = 0.02), whereas this relation was not significant for freezers (p = 0.15). Balance control is asymmetrical in most patients (about 75 percent) with Parkinson’s disease, but this asymmetry is not related to freezing. The relationship between weight bearing and balance control seems to be less pronounced in freezers, compared to healthy controls and non-freezers. However, this relationship should be investigated further in larger groups of patients. PMID:25032994

  14. Does anxiety cause freezing of gait in Parkinson's disease?

    PubMed

    Ehgoetz Martens, Kaylena A; Ellard, Colin G; Almeida, Quincy J

    2014-01-01

    Individuals with Parkinson's disease (PD) commonly experience freezing of gait under time constraints, in narrow spaces, and in the dark. One commonality between these different situations is that they may all provoke anxiety, yet anxiety has never been directly examined as a cause of FOG. In this study, virtual reality was used to induce anxiety and evaluate whether it directly causes FOG. Fourteen patients with PD and freezing of gait (Freezers) and 17 PD without freezing of gait (Non-Freezers) were instructed to walk in two virtual environments: (i) across a plank that was located on the ground (LOW), (ii) across a plank above a deep pit (HIGH). Multiple synchronized motion capture cameras updated participants' movement through the virtual environment in real-time, while their gait was recorded. Anxiety levels were evaluated after each trial using self-assessment manikins. Freezers performed the experiment on two separate occasions (in their ON and OFF state). Freezers reported higher levels of anxiety compared to Non-Freezers (p < 0.001) and all patients reported greater levels of anxiety when walking across the HIGH plank compared to the LOW (p < 0.001). Freezers experienced significantly more freezing of gait episodes (p = 0.013) and spent a significantly greater percentage of each trial frozen (p = 0.005) when crossing the HIGH plank. This finding was even more pronounced when comparing Freezers in their OFF state. Freezers also had greater step length variability in the HIGH compared to the LOW condition, while the step length variability in Non-Freezers did not change. In conclusion, this was the first study to directly compare freezing of gait in anxious and non-anxious situations. These results present strong evidence that anxiety is an important mechanism underlying freezing of gait and supports the notion that the limbic system may have a profound contribution to freezing in PD.

  15. SpeedyTime_7-Minus_Eighty_Degrees_Laboratory_Freezer_for_ ISS

    NASA Image and Video Library

    2017-08-23

    SpeedyTime 7 – Minus Eighty Degrees Laboratory Freezer for ISS Cutting-edge science is on the daily menu on board the International Space Station, but where do the astronauts store their lab results before they’re shipped back to Earth? In one of a dozen large freezers, of course: in this SpeedyTime segment, Expedition 52 flight engineer Jack Fischer shines a light on the MELFI, Minus Eighty Degrees Laboratory Freezer for ISS. _______________________________________ FOLLOW THE SPACE STATION! Twitter: https://twitter.com/Space_Station Facebook: https://www.facebook.com/ISS Instagram: https://instagram.com/iss/

  16. 75 FR 59469 - Energy Conservation Program: Energy Conservation Standards for Residential Refrigerators...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-27

    ...The Energy Policy and Conservation Act (EPCA) prescribes energy conservation standards for various consumer products and commercial and industrial equipment, including residential refrigerators, refrigerator-freezers, and freezers. EPCA also requires the U.S. Department of Energy (DOE) to determine whether more stringent, amended standards for these products are technologically feasible and economically justified, and would save a significant amount of energy. In this NOPR, DOE proposes amended energy conservation standards for residential refrigerators, refrigerator- freezers, and freezers. The NOPR also announces a public meeting to receive comment on these proposed standards and associated analyses and results.

  17. NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR FREEZER MAINTENANCE AND TEMPERATURE VERIFICATION FOR SAMPLE INTEGRITY (BCO-L-25.0)

    EPA Science Inventory

    The purpose of this SOP is to ensure suitable temperature maintenance of freezers used for storage of samples. This procedure was followed to ensure consistent data retrieval during the Arizona NHEXAS project and the "Border" study. Keywords: freezers; operation.

    The National H...

  18. Food Service Refrigerators and Food Service Storage Freezers. Standard No. 7, Revised April 1966.

    ERIC Educational Resources Information Center

    National Sanitation Foundation, Ann Arbor, MI.

    This standard covers the sanitation and performance requirements for new food service refrigerators and food service storage freezers of the type generally used in the food service industry. It covers cabinets operating in the freezers, above or below freezing temperature, designed for the storage or display of varieties of food products. Topics…

  19. Shuttle freezer conceptual design

    NASA Technical Reports Server (NTRS)

    Proctor, B. W.; Russell, D. J.

    1975-01-01

    A conceptual design for a kit freezer for operation onboard shuttle was developed. The freezer features a self-contained unit which can be mounted in the orbiter crew compartment and is capable of storing food at launch and returning with medical samples. Packaging schemes were investigated to provide the optimum storage capacity with a minimum weight and volume penalty. Several types of refrigeration systems were evaluated to select one which would offer the most efficient performance and lowest hazard of safety to the crew. Detailed performance data on the selected, Stirling cycle principled refrigeration unit were developed to validate the feasibility of its application to this freezer. Thermal analyses were performed to determine the adequacy of the thermal insulation to maintain the desired storage temperature with the design cooling capacity. Stress analyses were made to insure the design structure integrity could be maintained over the shuttle flight regime. A proposed prototype freezer development plan is presented.

  20. 76 FR 31795 - Energy Conservation Program: Energy Conservation Standards for Walk-In Coolers and Freezers...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-02

    .... That text, within the context of assessing the long-term thermal resistance of the insulating foam... foam insulation used in a walk-in freezer unit. The published temperature, 35 [deg]F 1 [deg]F--a... insulation foam used in walk- in freezers. See 42 U.S.C. 6314(a)(9)(A)(iii) (indicating that the insulation...

  1. 76 FR 57515 - Energy Conservation Program: Energy Conservation Standards for Residential Refrigerators...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-15

    ...The Energy Policy and Conservation Act (EPCA) prescribes energy conservation standards for various consumer products and commercial and industrial equipment, including refrigerators, refrigerator-freezers, and freezers. EPCA also requires the U.S. Department of Energy (DOE) to determine if more stringent, amended standards for these products are technologically feasible and economically justified, and would save a significant amount of energy. In this final rule, DOE is adopting more stringent energy conservation standards for refrigerators, refrigerator-freezers, and freezers. It has determined that the amended energy conservation standards for these products would result in the significant conservation of energy and are technologically feasible and economically justified.

  2. 10 CFR Appendix A to Subpart R of... - Uniform Test Method for the Measurement of Energy Consumption of the Components of Envelopes of...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...—Temperature Conditions Internal Temperatures (cooled space within the envelope) Cooler Dry Bulb Temperature 35...) Freezer and Cooler Dry Bulb Temperatures 75 °F. Subfloor Temperatures Freezer and Cooler Dry Bulb...,int,dp = dry-bulb air temperature internal to the cooler or freezer, °F, as prescribed in Table A.1...

  3. 10 CFR Appendix A to Subpart R of... - Uniform Test Method for the Measurement of Energy Consumption of the Components of Envelopes of...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...—Temperature Conditions Internal Temperatures (cooled space within the envelope) Cooler Dry Bulb Temperature 35...) Freezer and Cooler Dry Bulb Temperatures 75 °F. Subfloor Temperatures Freezer and Cooler Dry Bulb...,int,dp = dry-bulb air temperature internal to the cooler or freezer, °F, as prescribed in Table A.1...

  4. Solar Refrigerator/Freezers For Vaccines

    NASA Technical Reports Server (NTRS)

    Ratajczak, Anthony F.

    1988-01-01

    Report presents results of field tests of solar-cell-powered refrigerator/freezers for vaccines. Covers following topics: explanation of project; descriptions of refrigerator/freezer systems; account of installation experiences; performance data for 22 systems for which field-test data reported; summary of operational reliability; comments of users of some systems tested; and recommendations for design and future use. Photovoltaic systems store vaccines in remote regions where powerlines unavailable.

  5. Plastic freezer bags: a cost-effective method to protect extraction sites in laparoscopic colorectal procedures?

    PubMed

    Huynh, Hai P; Musselman, Reilly P; Trottier, Daniel C; Soto, Claudia M; Poulin, Eric C; Mamazza, Joseph; Boushey, Robin P; Auer, Rebecca C; Moloo, Husein

    2013-10-01

    To review surgical-site infection (SSI) and retrieval-site tumor recurrence rates in laparoscopic colorectal procedures when using a plastic freezer bag as a wound protector. Laparoscopic colorectal procedures where a plastic freezer bag used as a wound protector at the extraction site were reviewed between 1991 and 2008 from a prospectively collected database. χ test was used to compare SSI and tumor recurrence rates between groups. Costing data were obtained from the operating room supplies department. A total of 936 cases with 51 (5.45%) surgical-site infections were identified. SSI rates did not differ when comparing groups based on demographic factors, diagnosis, or location of procedure. Retrieval-site tumor recurrence rate was 0.21% (1/474). Cost of plastic freezer bags including sterilization ranged from $0.25 to $3. Plastic freezer bags as wound protectors in laparoscopic colorectal procedures are cost effective and have SSI and retrieval-site tumor recurrence rates that compare favorably to published data.

  6. 10 CFR Appendix A to Subpart R of... - Uniform Test Method for the Measurement of Energy Consumption of the Components of Envelopes of...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    .... Table A.1—Temperature Conditions Internal Temperatures (cooled space within the envelope) Cooler Dry... the envelope) Freezer and Cooler Dry Bulb Temperatures 75 °F. Subfloor Temperatures Freezer and Cooler... prescribed in Table A.1; and TDB,int,dp = dry-bulb air temperature internal to the cooler or freezer, °F, as...

  7. Freezer or non-freezer: clinical assessment of freezing of gait.

    PubMed

    Snijders, Anke H; Haaxma, Charlotte A; Hagen, Yolien J; Munneke, Marten; Bloem, Bastiaan R

    2012-02-01

    Freezing of gait (FOG) is both common and debilitating in patients with Parkinson's disease (PD). Future pathophysiology studies will depend critically upon adequate classification of patients as being either 'freezers' or 'non-freezers'. This classification should be based ideally upon objective confirmation by an experienced observer during clinical assessment. Given the known difficulties to elicit FOG when examining patients, we aimed to investigate which simple clinical test would be the most sensitive to provoke FOG objectively. We examined 50 patients with PD, including 32 off-state freezers (defined as experiencing subjective 'gluing of the feet to the floor'). Assessment including a FOG trajectory (three trials: normal speed, fast speed, and with dual tasking) and several turning variants (180° vs. 360° turns; leftward vs. rightward turns; wide vs. narrow turning; and slow vs. fast turns). Sensitivity of the entire assessment to provoke FOG in subjective freezers was 0.74, specificity was 0.94. The most effective test to provoke FOG was rapid 360° turns in both directions and, if negative, combined with a gait trajectory with dual tasking. Repeated testing improved the diagnostic yield. The least informative tests included wide turns, 180° turns or normal speed full turns. Sensitivity to provoke objective FOG in subjective freezers was 0.65 for the rapid full turns in both directions and 0.63 for the FOG trajectory. The most efficient way to objectively ascertain FOG is asking patients to repeatedly make rapid 360° narrow turns from standstill, on the spot and in both directions. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability.

    PubMed

    Schäfer-Somi, S; Palme, N

    2016-04-01

    The composition of seminal plasma and the localization of the ATP-binding cassette transporter A1 (ABCA1) in spermatozoa from good and bad freezers were compared to frozen-thawed spermatozoa from the same dog. Ejaculates were obtained from 31 stud dogs, and the sperm-rich fraction (SRF) was kept for analysis. One aliquot was used for the analysis of concentration, progressive motility (P; CASA), viability (V; CASA) and leucocyte count, and the analysis was performed by flow cytometry (FITC-PNA/PI), SCSA and HOST. In seminal plasma, concentration of albumin, cholesterol, calcium, inorganic phosphate, sodium, potassium, zinc and copper was measured. Semen smears were prepared and evaluated for the expression of ABCA1. The remainder of each ejaculate was frozen. After thawing, the quality assessment was repeated and further smears were prepared. According to post-thaw semen quality, dogs were assigned to good freezers (n = 20) or bad freezers (n = 11), the latter were defined as < 50% progressive motility and/or > 40% morphologically abnormal sperm and/or < 50% viability. Bad freezers were older than good freezers (5.3 vs 3.4 years, p < 0.05). In bad freezers, the percentage of sperm with ABCA1 signal in the acrosome was lower (26.3% vs 35.7%, p < 0.01) and the percentage of sperm with complete loss of ABCA1 signal higher (46.7% vs 30%, p < 0.01); the percentage of dead spermatozoa was higher (36.1% vs 25.5%, p < 0.05), and the concentration of cholesterol and sodium in seminal plasma was lower than in good freezers (p < 0.05). We conclude that in thawed bad freezer sperm, an increase in acrosome damages coincided with an increased loss of cholesterol transporters and cell death, and a lower cholesterol concentration in seminal plasma. Follow-up studies revealed whether a relation exists between these findings. © 2016 Blackwell Verlag GmbH.

  9. Freezing of Gait in Parkinson’s Disease: An Overload Problem?

    PubMed Central

    Beck, Eric N.; Ehgoetz Martens, Kaylena A.; Almeida, Quincy J.

    2015-01-01

    Freezing of gait (FOG) is arguably the most severe symptom associated with Parkinson’s disease (PD), and often occurs while performing dual tasks or approaching narrowed and cluttered spaces. While it is well known that visual cues alleviate FOG, it is not clear if this effect may be the result of cognitive or sensorimotor mechanisms. Nevertheless, the role of vision may be a critical link that might allow us to disentangle this question. Gaze behaviour has yet to be carefully investigated while freezers approach narrow spaces, thus the overall objective of this study was to explore the interaction between cognitive and sensory-perceptual influences on FOG. In experiment #1, if cognitive load is the underlying factor leading to FOG, then one might expect that a dual-task would elicit FOG episodes even in the presence of visual cues, since the load on attention would interfere with utilization of visual cues. Alternatively, if visual cues alleviate gait despite performance of a dual-task, then it may be more probable that sensory mechanisms are at play. In compliment to this, the aim of experiment#2 was to further challenge the sensory systems, by removing vision of the lower-limbs and thereby forcing participants to rely on other forms of sensory feedback rather than vision while walking toward the narrow space. Spatiotemporal aspects of gait, percentage of gaze fixation frequency and duration, as well as skin conductance levels were measured in freezers and non-freezers across both experiments. Results from experiment#1 indicated that although freezers and non-freezers both walked with worse gait while performing the dual-task, in freezers, gait was relieved by visual cues regardless of whether the cognitive demands of the dual-task were present. At baseline and while dual-tasking, freezers demonstrated a gaze behaviour that neglected the doorway and instead focused primarily on the pathway, a strategy that non-freezers adopted only when performing the dual-task. Interestingly, with the combination of visual cues and dual-task, freezers increased the frequency and duration of fixations toward the doorway, compared to non-freezers. These results suggest that although increasing demand on attention does significantly deteriorate gait in freezers, an increase in cognitive demand is not exclusively responsible for freezing (since visual cues were able to overcome any interference elicited by the dual-task). When vision of the lower limbs was removed in experiment#2, only the freezers’ gait was affected. However, when visual cues were present, freezers’ gait improved regardless of the dual-task. This gait behaviour was accompanied by greater amount of time spent looking at the visual cues irrespective of the dual-task. Since removing vision of the lower-limbs hindered gait even under low attentional demand, restricted sensory feedback may be an important factor to the mechanisms underlying FOG. PMID:26678262

  10. Modeling of the effect of freezer conditions on the hardness of ice cream using response surface methodology.

    PubMed

    Inoue, K; Ochi, H; Habara, K; Taketsuka, M; Saito, H; Ichihashi, N; Iwatsuki, K

    2009-12-01

    The effect of conventional continuous freezer parameters [mix flow (L/h), overrun (%), drawing temperature ( degrees C), cylinder pressure (kPa), and dasher speed (rpm)] on the hardness of ice cream under varying measured temperatures (-5, -10, and -15 degrees C) was investigated systematically using response surface methodology (central composite face-centered design), and the relationships were expressed as statistical models. The range (maximum and minimum values) of each freezer parameter was set according to the actual capability of the conventional freezer and applicability to the manufacturing process. Hardness was measured using a penetrometer. These models showed that overrun and drawing temperature had significant effects on hardness. The models can be used to optimize freezer conditions to make ice cream of the least possible hardness under the highest overrun (120%) and a drawing temperature of approximately -5.5 degrees C (slightly warmer than the lowest drawing temperature of -6.5 degrees C) within the range of this study. With reference to the structural elements of the ice cream, we suggest that the volume of overrun and ice crystal content, ice crystal size, and fat globule destabilization affect the hardness of ice cream. In addition, the combination of a simple instrumental parameter and response surface methodology allows us to show the relation between freezer conditions and one of the most important properties-hardness-visually and quantitatively on the practical level.

  11. Contemporary programs in support of traditional ways: Inuit perspectives on community freezers as a mechanism to alleviate pressures of wild food access in Nain, Nunatsiavut.

    PubMed

    Organ, Jennifer; Castleden, Heather; Furgal, Chris; Sheldon, Tom; Hart, Catherine

    2014-11-01

    Rapid socio-cultural, economic, and environmental changes are challenging wild food access and thus food security for Inuit in the Canadian Arctic. In response to the continued value and practice of harvesting wild foods, communities are establishing "wild food support" initiatives. This study evaluated how one such initiative, a community freezer, in Nain, Nunatsiavut supported wild food access for community members. Data were collected through: interviews and focus groups with users, freezer managers, and active harvesters; participant observation; and document analysis. Results indicated that the community freezer supported socio-cultural, economic and local access to wild foods. However, there were issues associated with supply, dependency, social exclusion, and tension between feasibility and traditional values and practices. Communities, governments, and policymakers are urged to consider social and physical location as factors when investing in and monitoring such initiatives. The Nunatsiavut Government and the Nain Inuit Community Government have since worked together to modify this early freezer initiative due, in part, to this study's findings. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. 7 CFR 58.311 - Coolers and freezers.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Rooms and Compartments § 58... further processing. Coolers and freezers shall be kept clean, orderly, free from insects, rodents, and...

  13. 7 CFR 58.311 - Coolers and freezers.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Rooms and Compartments § 58... further processing. Coolers and freezers shall be kept clean, orderly, free from insects, rodents, and...

  14. 7 CFR 58.311 - Coolers and freezers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Rooms and Compartments § 58... further processing. Coolers and freezers shall be kept clean, orderly, free from insects, rodents, and...

  15. 7 CFR 58.311 - Coolers and freezers.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Rooms and Compartments § 58... further processing. Coolers and freezers shall be kept clean, orderly, free from insects, rodents, and...

  16. 7 CFR 58.311 - Coolers and freezers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Rooms and Compartments § 58... further processing. Coolers and freezers shall be kept clean, orderly, free from insects, rodents, and...

  17. 9 CFR 354.221 - Rooms and compartments.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) Coolers and freezers. Coolers and freezers of adequate size and capacity shall be provided to reduce the... ventilated. (e) Storage and supply rooms. The storage and supply rooms shall be in good repair, kept dry, and...

  18. 9 CFR 354.221 - Rooms and compartments.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) Coolers and freezers. Coolers and freezers of adequate size and capacity shall be provided to reduce the... ventilated. (e) Storage and supply rooms. The storage and supply rooms shall be in good repair, kept dry, and...

  19. 9 CFR 354.221 - Rooms and compartments.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) Coolers and freezers. Coolers and freezers of adequate size and capacity shall be provided to reduce the... ventilated. (e) Storage and supply rooms. The storage and supply rooms shall be in good repair, kept dry, and...

  20. 9 CFR 354.221 - Rooms and compartments.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) Coolers and freezers. Coolers and freezers of adequate size and capacity shall be provided to reduce the... ventilated. (e) Storage and supply rooms. The storage and supply rooms shall be in good repair, kept dry, and...

  1. 9 CFR 354.221 - Rooms and compartments.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Coolers and freezers. Coolers and freezers of adequate size and capacity shall be provided to reduce the... ventilated. (e) Storage and supply rooms. The storage and supply rooms shall be in good repair, kept dry, and...

  2. 10 CFR 429.14 - Residential refrigerators, refrigerator-freezers and freezers.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... anti-sweat heater control (in which case, manufacturers must also report the values of heater Watts at the ten humidity levels 5%, 15%, through 95% used to calculate the variable anti-sweat heater...

  3. 10 CFR 429.14 - Residential refrigerators, refrigerator-freezers and freezers.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... anti-sweat heater control (in which case, manufacturers must also report the values of heater Watts at the ten humidity levels 5%, 15%, through 95% used to calculate the variable anti-sweat heater...

  4. 10 CFR 429.14 - Residential refrigerators, refrigerator-freezers and freezers.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... anti-sweat heater control (in which case, manufacturers must also report the values of heater Watts at the ten humidity levels 5%, 15%, through 95% used to calculate the variable anti-sweat heater...

  5. BRIC-12,Mission Specialist Tognini handles the GN2 freezer.

    NASA Image and Video Library

    1999-07-24

    S93-E-5006 (23 July 1999) --- Astronaut Michel Tognini, mission specialist representing the French space agency (CNES), opens the gaseous nitorgen (GN2) freezer on Columbia's middeck. The freezer is flown in support of two plant growth experiments--Plant Growth Investigations in Microgravity (PGIM) and Biological Research in Canisters (BRIC). Throughout the mission Tognini periodically freezes samples from the experiments to provide glimpses of the plants in various stages of development. The photo was recorded with an electronic still camera (ESC) on Flight Day 1.

  6. 10 CFR 431.302 - Definitions concerning walk-in coolers and walk-in freezers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... FOR CERTAIN COMMERCIAL AND INDUSTRIAL EQUIPMENT Walk-in Coolers and Walk-in Freezers § 431.302...; however the terms do not include products designed and marketed exclusively for medical, scientific, or...

  7. 16 CFR 305.11 - Labeling for refrigerators, refrigerator-freezers, freezers, dishwashers, clothes washers, water...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... headline, copy, and charts to maintain uniformity for immediate consumer recognition and readability. Trim... faces to be used are indicated on the prototype labels. No hyphenation should be used in setting...

  8. 16 CFR 305.11 - Labeling for refrigerators, refrigerator-freezers, freezers, dishwashers, clothes washers, water...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... headline, copy, and charts to maintain uniformity for immediate consumer recognition and readability. Trim... faces to be used are indicated on the prototype labels. No hyphenation should be used in setting...

  9. 16 CFR 305.11 - Labeling for refrigerators, refrigerator-freezers, freezers, dishwashers, clothes washers, water...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... headline, copy, and charts to maintain uniformity for immediate consumer recognition and readability. Trim... faces to be used are indicated on the prototype labels. No hyphenation should be used in setting...

  10. 16 CFR 305.11 - Labeling for refrigerators, refrigerator-freezers, freezers, dishwashers, clothes washers, water...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... headline, copy, and charts to maintain uniformity for immediate consumer recognition and readability. Trim... faces to be used are indicated on the prototype labels. No hyphenation should be used in setting...

  11. REVIEW OF ENERGY EFFICIENCY OF REFRIGERATOR/FREEZER GASKETS

    EPA Science Inventory

    The report gives results of an investigation of the significance of heat leakage through gaskets in household refrigerator/freezers, explores different design features, and suggests further study if necessary. he report gives results of an extensive literature review, interviews ...

  12. REVIEW OF ENERGY EFFICIENCY OF REFRIGERATOR/FREEZER GASKETS

    EPA Science Inventory

    The report gives results of an investigation of the significance of heat leakage through gaskets in household refrigerator/freezers, explores different design features, and suggests further study if necessary. The report gives results of an extensive literature review, interviews...

  13. 16 CFR 305.11 - Labeling for refrigerators, refrigerator-freezers, freezers, dishwashers, clothes washers, water...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... immediate consumer recognition and readability. Trim size dimensions for all labels shall be as follows... on the label. Specific sizes and faces to be used are indicated on the prototype labels. No...

  14. NASA Advanced Refrigerator/Freezer Technology Development Project Overview

    NASA Technical Reports Server (NTRS)

    Cairelli, J. E.

    1995-01-01

    NASA Lewis Research Center (LeRC) has recently initiated a three-year project to develop the advanced refrigerator/freezer (R/F) technologies needed to support future life and biomedical sciences space experiments. Refrigerator/freezer laboratory equipment, most of which needs to be developed, is enabling to about 75 percent of the planned space station life and biomedical science experiments. These experiments will require five different classes of equipment; three storage freezers operating at -20 C, -70 C and less than 183 C, a -70 C freeze-dryer, and a cryogenic (less than 183 C) quick/snap freezer. This project is in response to a survey of cooling system technologies, performed by a team of NASA scientists and engineers. The team found that the technologies, required for future R/F systems to support life and biomedical sciences spaceflight experiments, do not exist at an adequate state of development and concluded that a program to develop the advanced R/F technologies is needed. Limitations on spaceflight system size, mass, and power consumption present a significant challenge in developing these systems. This paper presents some background and a description of the Advanced R/F Technology Development Project, project approach and schedule, general description of the R/F systems, and a review of the major R/F equipment requirements.

  15. Modeling of the effect of freezer conditions on the principal constituent parameters of ice cream by using response surface methodology.

    PubMed

    Inoue, K; Ochi, H; Taketsuka, M; Saito, H; Sakurai, K; Ichihashi, N; Iwatsuki, K; Kokubo, S

    2008-05-01

    A systematic analysis was carried out by using response surface methodology to create a quantitative model of the synergistic effects of conditions in a continuous freezer [mix flow rate (L/h), overrun (%), cylinder pressure (kPa), drawing temperature ( degrees C), and dasher speed (rpm)] on the principal constituent parameters of ice cream [rate of fat destabilization (%), mean air cell diameter (mum), and mean ice crystal diameter (mum)]. A central composite face-centered design was used for this study. Thirty-one combinations of the 5 above-mentioned freezer conditions were designed (including replicates at the center point), and ice cream samples were manufactured and examined in a continuous freezer under the selected conditions. The responses were the 3 variables given above. A quadratic model was constructed, with the freezer conditions as the independent variables and the ice cream characteristics as the dependent variables. The coefficients of determination (R(2)) were greater than 0.9 for all 3 responses, but Q(2), the index used here for the capability of the model for predicting future observed values of the responses, was negative for both the mean ice crystal diameter and the mean air cell diameter. Therefore, pruned models were constructed by removing terms that had contributed little to the prediction in the original model and by refitting the regression model. It was demonstrated that these pruned models provided good fits to the data in terms of R(2), Q(2), and ANOVA. The effects of freezer conditions were expressed quantitatively in terms of the 3 responses. The drawing temperature ( degrees C) was found to have a greater effect on ice cream characteristics than any of the other factors.

  16. 7 CFR 58.126 - Buildings.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., manufactured, handled, packaged or stored (except dry storage of packaged finished products and supplies) or in... materials not regularly used. (1) Coolers and freezers. Coolers and freezers where dairy products are stored shall be clean, reasonably dry and maintained at the proper uniform temperature and humidity to...

  17. 7 CFR 58.126 - Buildings.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., manufactured, handled, packaged or stored (except dry storage of packaged finished products and supplies) or in... materials not regularly used. (1) Coolers and freezers. Coolers and freezers where dairy products are stored shall be clean, reasonably dry and maintained at the proper uniform temperature and humidity to...

  18. 7 CFR 58.126 - Buildings.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., manufactured, handled, packaged or stored (except dry storage of packaged finished products and supplies) or in... materials not regularly used. (1) Coolers and freezers. Coolers and freezers where dairy products are stored shall be clean, reasonably dry and maintained at the proper uniform temperature and humidity to...

  19. 7 CFR 58.126 - Buildings.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., manufactured, handled, packaged or stored (except dry storage of packaged finished products and supplies) or in... materials not regularly used. (1) Coolers and freezers. Coolers and freezers where dairy products are stored shall be clean, reasonably dry and maintained at the proper uniform temperature and humidity to...

  20. 7 CFR 58.126 - Buildings.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., manufactured, handled, packaged or stored (except dry storage of packaged finished products and supplies) or in... materials not regularly used. (1) Coolers and freezers. Coolers and freezers where dairy products are stored shall be clean, reasonably dry and maintained at the proper uniform temperature and humidity to...

  1. 10 CFR 431.62 - Definitions concerning commercial refrigerators, freezers and refrigerator-freezers.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... transparent or solid doors, sliding or hinged doors, a combination of hinged, sliding, transparent, or solid... compressors, refrigerant condensers, condenser fans and motors, and factory supplied accessories. Self... more refrigerant compressors, refrigerant condensers, condenser fans and motors, and factory supplied...

  2. Evaluation of a Freezer Mill for Bone Pulverization prior to DNA Extraction: An Improved Workflow for STR Analysis.

    PubMed

    Morales Colón, Emely; Hernández, Mireya; Candelario, Mariel; Meléndez, María; Dawson Cruz, Tracey

    2018-03-01

    Traditional methods for bone pulverization typically generate heat, risking stability of DNA sample. SPEX™ has developed cryogenic grinders which introduce liquid nitrogen to cool the sample and aid in the grinding process. In this study, the Freezer Mill 6970 EFM was used with two DNA extraction methods and routine downstream STR analysis procedures. DNA from as little as 0.1 g of bone powder was used to develop full STR profiles after freezer mill pulverization, and the method was reproducible. Further, no contamination was detected upon cleaning/reuse of the sample vials. There were no significant differences in DNA yield, STR alleles detected, or peak heights using the freezer mill as compared to traditional grinding, and successful DNA profiles were achieved from as low as 0.1 g of bone powder with this method. Overall, this work indicates that this cryogenic mill method may be used as a viable alternative to traditional tissue grinders. © 2017 American Academy of Forensic Sciences.

  3. Design of a freezer system for Columbus

    NASA Astrophysics Data System (ADS)

    Vidal, P.; Ravex, A.; Villard, J. C.

    1991-12-01

    Refrigeration facilities are required to support life sciences experiments on the Columbus Space Station laboratories. Current freezers, achieving temperatures of about -20 C, are adequate for short mission times. Significantly lower temperatures, of the order of -80 C are required to avoid deterioration of specimens during the long mission times associated with Columbus utilization. A study which reviewed and traded available technologies to clearly identify and justify the choice of a freezer system providing cold storage at -20 and -80 C is presented. The design of the freezer system is then established and described. The technical solution is based on Dewar casing technology and Brayton cycle refrigeration. The Dewar technology ensures very low heat loads to the refrigeration machine and, therefore, a low overall power consumption. The Brayton cycle was chosen above all for its intrinsic microgravity compatibility. It also presents the high advantage of using nitrogen as heat carrier and of having low pressure ratios, which is a considerable safety bonus in a manned closed environment.

  4. Is sperm cryopreservation at -150 degree C a feasible alternative?

    PubMed

    Medrano, A; Cabrera, F; González, F; Batista, M; Gracia, A

    2002-01-01

    A series of experiments was carried out to validate a -150 degree C ultra-low temperature freezer for its possible use to properly freeze and store semen. In the first part, crude sample handling was simulated to see whether temperature of stored samples was maintained within a safe range; also, the freezing point and latent heat of fusion plateau of a semen extender were monitored. In the second part, buck semen was (i) frozen in liquid nitrogen and stored in the ultra-low freezer, (ii) frozen and stored in the ultra-low freezer, and (iii) frozen and stored in liquid nitrogen, to compare sperm cryosurvival between freezing methods. Both, frequent removal of samples and long opening of the freezer door did not negatively affect stored sample temperature; latent heat of fusion plateau was 5 minutes long. Semen stored either at -150 degree C or at -196 degree C cryosurvived similarly after 2 days and after 2 months of cryopreservation.

  5. 78 FR 55781 - Energy Conservation Program: Energy Conservation Standards for Walk-In Coolers and Freezers

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-11

    ...The Energy Policy and Conservation Act of 1975 (EPCA), as amended, prescribes energy conservation standards for various consumer products and certain commercial and industrial equipment, including walk-in coolers and walk-in freezers. EPCA also requires the U.S. Department of Energy (DOE) to determine whether more-stringent, amended standards would be technologically feasible and economically justified, and would save a significant amount of energy. In this notice, DOE proposes amended energy conservation standards for walk-in coolers and walk-in freezers. The notice also announces a public meeting to receive comment on these proposed standards and associated analyses and results.

  6. MELFI ops

    NASA Image and Video Library

    2015-05-18

    ISS043E207615 (05/18/2015) --- Expedition 43 Commander Terry Virts on the International Space Station works with experiment samples stored inside one of the station’s Minus Eighty-Degree Laboratory Freezer for ISS, or MELFI. These laboratory freezers are used to maintain experiment samples at ultra-cold temperatures throughout a mission.

  7. FLAME SUPPRESSION AND LUBRICANT INTERACTION OF HYDROCARBON MIXTURES FOR HOUSEHOLD REFRIGERATOR/FREEZERS

    EPA Science Inventory

    The paper discusses the flame suppression and lubricant interaction of hydrocarbon (HC) mixtures for household refrigerator/freezers (R/Fs). The work focuses on blending the minimum amount of a fluoroiodocarbon (FIC) with previously optimized R/F-tested HC mixtures to reduce thei...

  8. SIMULATION RESULTS OF SINGLE REFRIGERANTS FOR USE IN A DUAL-CIRCUIT REFRIGERATOR/FREEZER

    EPA Science Inventory

    The paper reviews the refrigerant/freezer (RF) design and refrigerant selection process that is necessary to design an energy efficient RF that does not use fully halogenated chlorofluorocarbons (CFCs). EPA is interested in phasing out CFCs in RFs to minimize stratospheric ozone ...

  9. 10 CFR 431.62 - Definitions concerning commercial refrigerators, freezers and refrigerator-freezers.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... occupancy sensor means a device which uses passive infrared, ultrasonic, or other motion-sensing technology..., frozen, combination chilled and frozen, or variable temperature; (4) Displays or stores merchandise and... doors; (6) Is designed for pull-down temperature applications or holding temperature applications; and...

  10. ADVANCED INSULATIONS FOR REFRIGERATOR/FREEZERS: THE POTENTIAL FOR NEW SHELL DESIGNS INCORPORATING POLYMER BARRIER CONSTRUCTION

    EPA Science Inventory

    The report examines domestic refrigerator/freezer (R/F) design alternatives which may offer greater increase in thermal performance than is possible with panel/foam composites. (NOTE: Current efforts to design and build R/Fs with high performance insulation technology are directe...

  11. PERFORMANCE OF A TWO-CYCLE REFRIGERATOR/FREEZER USING HFC REFRIGERANTS

    EPA Science Inventory

    A two-cycle 18 ft3 (0.51 m3) refrigerator/freezer (R/F) was tested utilizing American National Standards Institute (ANSI)/AHAM (1988) standards for energy consumption testing. A 34.9% energy consumption reduction was realized. This work presents a proven method of reducing the ...

  12. iss055e043245

    NASA Image and Video Library

    2018-04-30

    iss055e043245 (April 30, 2018) --- NASA astronaut Ricky Arnold transfers frozen biological samples from science freezers aboard the International Space Station to science freezers inside the SpaceX Dragon resupply ship. The research samples were returned to Earth aboard Dragon for retrieval by SpaceX engineers and analysis by NASA scientists.

  13. 76 FR 13973 - United States Warehouse Act; Processed Agricultural Products Licensing Agreement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-15

    ... license for the processed agricultural products that are stored in climate controlled, cooler, and freezer..., and dry beans. Warehouse operators voluntarily agree to be licensed, observe the rules for licensing... program for processed agricultural products that are stored in climate controlled, cooler, and freezer...

  14. 7 CFR 58.344 - Storage of finished product in freezer.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... maintained at −10 °F. or lower and shall be equipped to provide sufficient high velocity, air circulation for rapid freezing. After the products have been completely frozen, they may be transferred to a freezer..., if necessary, at a later time. Requirements for Finished Products Bearing USDA Official...

  15. 7 CFR 58.344 - Storage of finished product in freezer.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... maintained at −10 °F. or lower and shall be equipped to provide sufficient high velocity, air circulation for rapid freezing. After the products have been completely frozen, they may be transferred to a freezer..., if necessary, at a later time. Requirements for Finished Products Bearing USDA Official...

  16. 7 CFR 58.344 - Storage of finished product in freezer.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... maintained at −10 °F. or lower and shall be equipped to provide sufficient high velocity, air circulation for rapid freezing. After the products have been completely frozen, they may be transferred to a freezer..., if necessary, at a later time. Requirements for Finished Products Bearing USDA Official...

  17. 7 CFR 58.344 - Storage of finished product in freezer.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... maintained at −10 °F. or lower and shall be equipped to provide sufficient high velocity, air circulation for rapid freezing. After the products have been completely frozen, they may be transferred to a freezer..., if necessary, at a later time. Requirements for Finished Products Bearing USDA Official...

  18. 7 CFR 58.344 - Storage of finished product in freezer.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... maintained at −10 °F. or lower and shall be equipped to provide sufficient high velocity, air circulation for rapid freezing. After the products have been completely frozen, they may be transferred to a freezer..., if necessary, at a later time. Requirements for Finished Products Bearing USDA Official...

  19. Revisiting a Problem of Two Freezers

    ERIC Educational Resources Information Center

    Easton, Don

    2014-01-01

    The January 2013 Physics Challenge for Teachers and Students has some features that are surprising and worth a closer look. The problem concerns a Carnot-cycle refrigeration unit operating inside a tent. It achieves dynamic equilibrium with a freezer ("cold") compartment temperature of T[subscript C] = 13°C, tent temperature of…

  20. EPA'S RESEARCH PROJECTS RELATING TO THE DUAL-CIRCUIT AND LORENZ REFRIGERATOR/FREEZERS

    EPA Science Inventory

    The paper discusses EPA research projects relating to the dual-circuit and Lorenz refrigerator/freezers (RFs). EPA is interested in not only phasing out chlorofluorocarbons (CFCs) in RFs, but doing so in a way that will maximize energy efficiency and minimize subsequent impacts o...

  1. SYNOPSIS OF THE U.S. ENVIRONMENTAL PROTECTION AGENCY RESIDENTIAL REFRIGERATOR/FREEZER ALTERNATIVE REFRIGERANTS EVALUATION PROGRAM

    EPA Science Inventory

    The paper is a recapitulation of the experimental testing at the U.S. Environmental Protection Agency's NRMRL's (National Risk Management Research Laboratory's) Air Pollution Prevention and Control Division on residential refrigerator/freezers (R/Fs). R/F testing at the NRMRL lab...

  2. 16 CFR Appendix B3 to Part 305 - Chest Freezers and All Other Freezers

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  3. 16 CFR Appendix B3 to Part 305 - Chest Freezers and All Other Freezers

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  4. 16 CFR Appendix B3 to Part 305 - Chest Freezers and All Other Freezers

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  5. 16 CFR Appendix B3 to Part 305 - Chest Freezers and All Other Freezers

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  6. 76 FR 27610 - Approval and Promulgation of Air Quality Implementation Plans; Maryland; Adoption of Control...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ..., freezers, water heaters, dishwashers, trash compactors, air conditioners, ovens, microwave ovens, and other... appliance product. A large appliance product is also defined as any organic surface-coated metal range, oven, microwave, refrigerator, freezer, washer, dryer, dishwasher, water heater, or trash compactor manufactured...

  7. 10 CFR 431.62 - Definitions concerning commercial refrigerators, freezers and refrigerator-freezers.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... measurements taken during the test. Lighting occupancy sensor means a device which uses passive infrared...) Operates at a chilled, frozen, combination chilled and frozen, or variable temperature; (4) Displays or... doors, or no doors; (6) Is designed for pull-down temperature applications or holding temperature...

  8. 76 FR 29791 - Bottom Mount Combination Refrigerator-Freezers From Korea and Mexico

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-23

    ... INTERNATIONAL TRADE COMMISSION [Investigation Nos. 701-TA-477 and 731-TA-1180-1181 (Preliminary)] Bottom Mount Combination Refrigerator-Freezers From Korea and Mexico Determinations On the basis of the record \\1\\ developed in the subject investigations, the United States International Trade Commission (Commission) determines, pursuant to section...

  9. Fort Meade demonstration test LEDS in freezer rooms, fiber optics in display cases

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Parker, Steven; Parker, Graham B.

    2008-10-25

    Demonstration projects at Fort George G. Meade, MD, substituted LED lighting for incandescent bulbs in commisary wal-in freezers and fiber optic lighting in reach-in display cases. The goal was to reduce energy consumption and the results were positive. Journal article published in Public Works Digest

  10. EVALUATION OF PERFORMANCE AND COMPOSITION SHIFT OF ZEOTROPIC MIXTURES IN A LORENZ-MEUTZNER REFRIGERATOR/FREEZER

    EPA Science Inventory

    Results from previous testing of this refrigerator/freezer (R/F) using a 750 Btu/hr compressor and several zeotrophic mixtures revealed a performance enhancement up to 16 percent above that of HFC-134a. In the study presented in this paper, the Lorenz-Meutzner (LM) R/F equipped ...

  11. Bridging Philosophy of Technology and Neurobiological Research: Interpreting Images from the "Slam Freezer"

    ERIC Educational Resources Information Center

    Rosenberger, Robert

    2005-01-01

    The swiftly growing field of neurobiological research utilizes highly advanced technologies (e.g., magnetic resonance imaging, electron microscopy) to mediate between investigators and the brains they investigate. Here, the author analyzes a device called the "slam freezer" that quick-freezes neurons to be studied under the microscope. Employing…

  12. 10 CFR 431.302 - Definitions concerning walk-in coolers and walk-in freezers.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... walk-in cooler or walk-in freezer that are not part of its refrigeration system. K-factor means the... consumption, including, but not limited to, refrigeration, doors, lights, windows, or walls; or (2... temperature at or below 55 degrees Fahrenheit using a refrigeration system. Refrigeration system means the...

  13. 10 CFR 431.302 - Definitions concerning walk-in coolers and walk-in freezers.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... walk-in cooler or walk-in freezer that are not part of its refrigeration system. K-factor means the... consumption, including, but not limited to, refrigeration, doors, lights, windows, or walls; or (2... temperature at or below 55 degrees Fahrenheit using a refrigeration system. Refrigeration system means the...

  14. SIMULATION OF NON-AZEOTROPIC REFRIGERANT MIXTURES FOR USE IN A DUAL-CIRCUIT REFRIGERATOR/FREEZER WITH COUNTERCURRENT HEAT EXCHANGES

    EPA Science Inventory

    The paper discusses a refrigerator/freezer (RF) system that has two complete and independent refrigeration cycles for the two compartments. It uses a non-azeotropic refrigerant mixture (NARM) in each cycle and countercurrent heat exchangers throughout. This RF is housed in a stan...

  15. 10 CFR 431.302 - Definitions concerning walk-in coolers and walk-in freezers.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... walk-in cooler or walk-in freezer that are not part of its refrigeration system. K-factor means the... consumption, including, but not limited to, refrigeration, doors, lights, windows, or walls; or (2... temperature at or below 55 degrees Fahrenheit using a refrigeration system. Refrigeration system means the...

  16. 78 FR 35898 - Decision and Order Granting a Waiver to Samsung From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-14

    ... Refrigerator-Freezer Test Procedures AGENCY: Office of Energy Efficiency and Renewable Energy, Department of... from the DOE electric refrigerator and refrigerator-freezer test procedures for specific basic models set forth in its petition for waiver. In its petition, Samsung provides an alternate test procedure...

  17. 78 FR 35901 - Decision and Order Granting a Waiver to Samsung From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-14

    ... Refrigerator-Freezer Test Procedures AGENCY: Office of Energy Efficiency and Renewable Energy, Department of... from the DOE electric refrigerator and refrigerator-freezer test procedures for specific basic models set forth in its petition for waiver. In its petition, Samsung provides an alternate test procedure...

  18. 77 FR 63719 - Fisheries of the Exclusive Economic Zone Off Alaska; Monitoring and Enforcement Requirements in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... published on September 26, 2012, modifying equipment and operational requirements for freezer longliners... Pacific cod at sea with hook-and-line gear in the Bering Sea and Aleutian Islands Management Area (BSAI... modifying equipment and operational requirements for freezer longliners published September 26, 2012, NMFS...

  19. 10 CFR 431.306 - Energy conservation standards and their effective dates.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., ceiling, and door insulation of at least R-25 for coolers and R-32 for freezers, except that this... energy consumption; (4) Contain floor insulation of at least R-28 for freezers; (5) For evaporator fan... triple-pane glass with either heat-reflective treated glass or gas fill. (2) Transparent reach-in doors...

  20. 75 FR 186 - Energy Conservation Program: Test Procedures for Walk-In Coolers and Walk-In Freezers

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... freezers: (i) The R value shall be the 1/K factor multiplied by the thickness of the panel. (ii) The K... signed original paper copy. 4. Hand Delivery/Courier: Ms. Brenda Edwards, U.S. Department of Energy... signed original paper copy. For detailed instructions on submitting comments and additional information...

  1. NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR MAINTENANCE AND TEMPERATURE VERIFICATION OF REFRIGERATED UNITS FOR SAMPLE STORAGE (UA-L-4.1)

    EPA Science Inventory

    The purpose of this SOP is to assure suitable temperature maintenance in refrigerators and freezers used for sample storage during the Arizona NHEXAS project and the "Border" study. Keywords: lab; equipment; refrigerators and freezers.

    The National Human Exposure Assessment Su...

  2. 10 CFR Appendix B1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... defrost control. The energy consumption in kilowatt-hours per day shall be calculated equivalent to: ET... though the consumer-activated compartment temperature controls were preset so that the desired... calculated effect of operating a freezer for a period of 24 hours with the consumer-activated controls other...

  3. Factors affecting the energy consumption of two refrigerator-freezers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kao, J.Y.; Kelley, G.E.

    Two refrigerator-freezers, one with a top-mounted freezer and one with side-by-side doors, were tested in the laboratory to determine the sensitivity of their energy consumption to various operational factors. Room temperature, room humidity, door openings, and the setting of the anti-sweat heater switch were the factors examined. The results indicated that the room temperature and door openings had a significantly greater effect on energy consumption than the other two factors. More detailed tests were then performed under different room temperature and door-opening combinations. The relationship of door openings and the equivalent test room temperature was established. Finally, the effect onmore » energy of different temperature settings was studied. Test results are presented and discussed.« less

  4. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sikes, Karen; Blackburn, Julia; Grubbs, Tyler

    Despite a steady record of energy efficiency improvements in residential refrigerators and freezers over recent decades, these products still account for 4% of the site energy consumption for the average U.S. household. The Oak Ridge National Laboratory (ORNL) – along with partners Sandia National Laboratories (SNL) and the University of Maryland – are pursuing further efficiency improvements in this market sector by using a novel/prototype rotating heat exchanger (RHX) based on a Sandia Cooler technology as an evaporator in a residential refrigerator-freezer. The purpose of this study is to investigate the market potential of refrigerator-freezer products equipped with RHX evaporatorsmore » in the United States, including projections of maximum annual market share and unit shipments and maximum direct and indirect job creation.« less

  5. Effects of Extended Freezer Storage on the Integrity of Human Milk.

    PubMed

    Ahrabi, Ali Faraghi; Handa, Deepali; Codipilly, Champa N; Shah, Syed; Williams, Janet E; McGuire, Mark A; Potak, Debra; Aharon, Grace Golda; Schanler, Richard J

    2016-10-01

    To examine the integrity (pH, bacterial counts, host defense factors, nutrient contents, and osmolality) of freshly expressed and previously refrigerated human milk subjected to long-term freezer storage. Mothers donated 100 mL of freshly expressed milk. Samples were divided into baseline, storage at -20°C (fresh frozen) for 1, 3, 6, and 9 months, and prior storage at +4°C for 72 hours (refrigerated frozen) before storage at -20°C for 1 to 9 months. Samples were analyzed for pH, total bacterial colony count, gram-positive and gram-negative colony counts, and concentrations of total protein, fat, nonesterified fatty acids, lactoferrin, secretory IgA, and osmolality. Milk pH, total bacterial colony count, and Gram-positive colony counts decreased significantly with freezer storage (P < .001); bacterial counts decreased most rapidly in the refrigerated frozen group. The gram-negative colony count decreased significantly over time (P < .001). Nonesterified fatty acid concentrations increased significantly with time in storage (P < .001). Freezing for up to 9 months did not affect total protein, fat, lactoferrin, secretory IgA, or osmolality in either group. Freezer storage of human milk for 9 months at -20°C is associated with decreasing pH and bacterial counts, but preservation of key macronutrients and immunoactive components, with or without prior refrigeration for 72 hours. These data support current guidelines for freezer storage of human milk for up to 9 months for both freshly expressed and refrigerated milk. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR MAINTENANCE AND TEMPERATURE VERIFICATION OF REFRIGERATED UNITS FOR SAMPLE STORAGE (UA-L-4.1)

    EPA Science Inventory

    The purpose of this SOP is to assure suitable temperature maintenance in refrigerators and freezers used for sample storage during the Arizona NHEXAS project and the Border study. Keywords: lab; equipment; refrigerators and freezers.

    The U.S.-Mexico Border Program is sponsored...

  7. 10 CFR Appendix B to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 3 2013-01-01 2013-01-01 false Uniform Test Method for Measuring the Energy Consumption... ENERGY CONSERVATION PROGRAM FOR CONSUMER PRODUCTS Test Procedures Pt. 430, Subpt. B, App. B Appendix B to Subpart B of Part 430—Uniform Test Method for Measuring the Energy Consumption of Freezers The provisions...

  8. 10 CFR Appendix B to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 3 2012-01-01 2012-01-01 false Uniform Test Method for Measuring the Energy Consumption... ENERGY CONSERVATION PROGRAM FOR CONSUMER PRODUCTS Test Procedures Pt. 430, Subpt. B, App. B Appendix B to Subpart B of Part 430—Uniform Test Method for Measuring the Energy Consumption of Freezers The provisions...

  9. 10 CFR Appendix B to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 3 2011-01-01 2011-01-01 false Uniform Test Method for Measuring the Energy Consumption... ENERGY CONSERVATION PROGRAM FOR CONSUMER PRODUCTS Test Procedures Pt. 430, Subpt. B, App. B Appendix B to Subpart B of Part 430—Uniform Test Method for Measuring the Energy Consumption of Freezers The provisions...

  10. Highly Conductive Carbon Fiber Reinforced Concrete for Icing Prevention and Curing.

    PubMed

    Galao, Oscar; Bañón, Luis; Baeza, Francisco Javier; Carmona, Jesús; Garcés, Pedro

    2016-04-12

    This paper aims to study the feasibility of highly conductive carbon fiber reinforced concrete (CFRC) as a self-heating material for ice formation prevention and curing in pavements. Tests were carried out in lab ambient conditions at different fixed voltages and then introduced in a freezer at -15 °C. The specimens inside the freezer were exposed to different fixed voltages when reaching +5 °C for prevention of icing and when reaching the temperature inside the freezer, i.e. , -15 °C, for curing of icing. Results show that this concrete could act as a heating element in pavements with risk of ice formation, consuming a reasonable amount of energy for both anti-icing (prevention) and deicing (curing), which could turn into an environmentally friendly and cost-effective deicing method.

  11. Photovoltaic-Powered Vaccine Refrigerator: Freezer Systems Field Test Results

    NASA Technical Reports Server (NTRS)

    Ratajczak, A. F.

    1985-01-01

    A project to develop and field test photovoltaic-powered refrigerator/freezers suitable for vaccine storage was undertaken. Three refrigerator/freezers were qualified; one by Solar Power Corp. and two by Solvolt. Follow-on contracts were awarded for 19 field test systems and for 10 field test systems. A total of 29 systems were installed in 24 countries between October 1981 and October 1984. The project, systems descriptions, installation experiences, performance data for the 22 systems for which field test data was reported, an operational reliability summary, and recommendations relative to system designs and future use of such systems are explained. Performance data indicate that the systems are highly reliable and are capable of maintaining proper vaccine storage temperatures in a wide range of climatological and user environments.

  12. Whitson working on the Nutrition Experiment during Expedition 15/Expedition 16

    NASA Image and Video Library

    2007-10-19

    ISS015-E-35308 (19 Oct. 2007) --- NASA astronaut Peggy A. Whitson, Expedition 16 commander, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.

  13. 10 CFR Appendix B to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... factor. 1.2 “Anti-sweat heater” means a device incorporated into the design of a freezer to prevent the accumulation of moisture on exterior or interior surfaces of the cabinet. 1.3 “Anti-sweat heater switch” means a user-controllable switch or user interface which modifies the activation or control of anti-sweat...

  14. Evaluation of vaccine cold chain in urban health centers of municipal corporation of surat city, Western India.

    PubMed

    Naik, Ashish K; Rupani, Mihir P; Bansal, R K

    2013-12-01

    The success of immunization depends highly on the level of cold chain maintenance. The aim of the study was to assess the condition of cold chain equipment, practices adopted for cold chain maintenance and knowledge of the vaccinators. It was a cross-sectional study conducted in 20 UHCs of Surat Municipal Corporation (SMC). Cold chain equipment were observed with regards to their condition, along with the practices adopted by vaccinators for cold chain maintenance. A pre-designed and pre-tested questionnaire was used to interview the vaccinators regarding their knowledge and awareness regarding cold chain practices, management and handling. Data were entered and analyzed using Epi Info v 3.5.1. Simple proportions were calculated. Absence of separate stabilizer for deep freezers and ILRs (85%), ill-maintained temperature-record register, lack of criss-cross pattern of ice packs in deep freezer (65%), presence of things other than ice packs in deep freezer (10%) and things other than vaccines in ILR (10%) indicate poor cold chain maintenance. In addition to this, expired vaccines in ILR (5%), vaccines in the "unusable" stages of VVM (15%), lack of emergency contact number nearby in case of cold chain failure (85%), lack of inverter (85%), lack of generator (85%) and failure to note time of reconstitution on the vaccine vial at the time of vaccination (25%) indicate poor cold chain practices. Lack of knowledge of defrosting of ILR and deep freezer (45%), lack of knowledge about Shake test (40%), lack of knowledge of temperature range to be maintained in deep freezer (70%) and in ILR (15%) indicate poor knowledge of vaccinators. Cold chain maintenance and practices need improvement. Knowledge of vaccinators was overall unsatisfactory.

  15. A Neurocomputational Model of the Effect of Cognitive Load on Freezing of Gait in Parkinson's Disease.

    PubMed

    Muralidharan, Vignesh; Balasubramani, Pragathi P; Chakravarthy, V Srinivasa; Gilat, Moran; Lewis, Simon J G; Moustafa, Ahmed A

    2016-01-01

    Experimental data show that perceptual cues can either exacerbate or ameliorate freezing of gait (FOG) in Parkinson's Disease (PD). For example, simple visual stimuli like stripes on the floor can alleviate freezing whereas complex stimuli like narrow doorways can trigger it. We present a computational model of the cognitive and motor cortico-basal ganglia loops that explains the effects of sensory and cognitive processes on FOG. The model simulates strong causative factors of FOG including decision conflict (a disagreement of various sensory stimuli in their association with a response) and cognitive load (complexity of coupling a stimulus with downstream mechanisms that control gait execution). Specifically, the model simulates gait of PD patients (freezers and non-freezers) as they navigate a series of doorways while simultaneously responding to several Stroop word cues in a virtual reality setup. The model is based on an actor-critic architecture of Reinforcement Learning involving Utility-based decision making, where Utility is a weighted sum of Value and Risk functions. The model accounts for the following experimental data: (a) the increased foot-step latency seen in relation to high conflict cues, (b) the high number of motor arrests seen in PD freezers when faced with a complex cue compared to the simple cue, and (c) the effect of dopamine medication on these motor arrests. The freezing behavior arises as a result of addition of task parameters (doorways and cues) and not due to inherent differences in the subject group. The model predicts a differential role of risk sensitivity in PD freezers and non-freezers in the cognitive and motor loops. Additionally this first-of-its-kind model provides a plausible framework for understanding the influence of cognition on automatic motor actions in controls and Parkinson's Disease.

  16. A Neurocomputational Model of the Effect of Cognitive Load on Freezing of Gait in Parkinson's Disease

    PubMed Central

    Muralidharan, Vignesh; Balasubramani, Pragathi P.; Chakravarthy, V. Srinivasa; Gilat, Moran; Lewis, Simon J. G.; Moustafa, Ahmed A.

    2017-01-01

    Experimental data show that perceptual cues can either exacerbate or ameliorate freezing of gait (FOG) in Parkinson's Disease (PD). For example, simple visual stimuli like stripes on the floor can alleviate freezing whereas complex stimuli like narrow doorways can trigger it. We present a computational model of the cognitive and motor cortico-basal ganglia loops that explains the effects of sensory and cognitive processes on FOG. The model simulates strong causative factors of FOG including decision conflict (a disagreement of various sensory stimuli in their association with a response) and cognitive load (complexity of coupling a stimulus with downstream mechanisms that control gait execution). Specifically, the model simulates gait of PD patients (freezers and non-freezers) as they navigate a series of doorways while simultaneously responding to several Stroop word cues in a virtual reality setup. The model is based on an actor-critic architecture of Reinforcement Learning involving Utility-based decision making, where Utility is a weighted sum of Value and Risk functions. The model accounts for the following experimental data: (a) the increased foot-step latency seen in relation to high conflict cues, (b) the high number of motor arrests seen in PD freezers when faced with a complex cue compared to the simple cue, and (c) the effect of dopamine medication on these motor arrests. The freezing behavior arises as a result of addition of task parameters (doorways and cues) and not due to inherent differences in the subject group. The model predicts a differential role of risk sensitivity in PD freezers and non-freezers in the cognitive and motor loops. Additionally this first-of-its-kind model provides a plausible framework for understanding the influence of cognition on automatic motor actions in controls and Parkinson's Disease. PMID:28119584

  17. Introduction Analysis of Refrigerating and Air-Conditioning Technologies in Micro Grid Type Food Industrial Park

    NASA Astrophysics Data System (ADS)

    Shimazaki, Yoichi

    The aim of this study was to evaluate the refrigerating and air-conditioning technologies in cases of introducing both cogeneration system and energy network in food industrial park. The energy data of 14 factories were classified into steam, hot water, heating, cooling, refrigerating, freezing and electric power by interviews. The author developed a micro grid model based on linear programming so as to minimize the total system costs. The industrial park was divided into the 2,500 square meter mesh in order to take steam transport into consideration. Four cases were investigated. It was found that the electric power driven freezer was introduced compared with the ammonia absorption freezer. The ammonia absorption freezer was introduced in the factory that there is a little steam demand and large freezing demand at the same time.

  18. The Freon loop double containment design for Spacelab refrigerator/freezer to protect environment

    NASA Technical Reports Server (NTRS)

    Hye, A.

    1985-01-01

    General Electric is building a vapor compression refrigerator/freezer for NASA-Johnson Space Center for the Spacelab mission SLS-1 for life sciences experiments. Freon R502 is used as refrigerant. As R502 is considered toxic, the whole Freon loop is enclosed in a second containment to avoid exposure to crewmen. A detailed description of the design and construction of the safety enclosure is presented.

  19. Food and medical sample freezer kit concept for Shuttle

    NASA Technical Reports Server (NTRS)

    Copeland, R. J.; Jaax, J. R.; Proctor, B. W.

    1977-01-01

    A variety of food and storage of samples can be provided by a Space Shuttle Orbiter Freezer Kit. The proposed concept is an integrated package consisting of four -23 C (-10 F) storage compartments and a Stirling cycle refrigeration unit. The Stirling cycle mechanical refrigeration was selected over alternative systems for superior efficiency and safety. The trade-offs and a conceptual design of the system are presented.

  20. Epidemic Outbreak Surveillance (EOS)

    DTIC Science & Technology

    2006-07-01

    Experiment to Test Integrity of2003 Nasal Wash and Throat Swab Samples Stored at -80°C. September 2005 (Sue WorthyLuke Daum) Seven matched pairs of...Nasal Wash and Throat Swab samples, previously tested in September 2004 for Influenza A, were pulled from the -80°C storage freezer for re-testing...January 2006 a detailed vrocess was completed to inventorv and organize the sample storage freezers for all Nasal wash, Throat swab and PAXgene

  1. Analysis of a domestic refrigerator cycle with an ejector

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tomasek, M.L.; Radermacher, R.

    1995-08-01

    In this paper, an improved cooling cycle for a conventional domestic refrigerator-freezer utilizing an ejector for vapor precompression is analyzed using an idealized model Its energy efficiency is compared to that of the conventional refrigerator-freezer system. Emphasis is placed on off-design conditions. The ejector-enhanced refrigeration cycle consists of two evaporators that operate at different pressure and temperature levels. The ejector combines the vapor flows exiting the two evaporators into one at an intermediate pressure level The ejector cycle gives an increase of up to 12.4% in the coefficient of performance (COP) compared to that of a standard refrigerator-freezer refrigeration cycle.more » The analysis includes calculations on the optimum throat diameters of the ejector. The investigation on the off-design performance of the ejector cycle shows little dependency of energy consumption on constant ejector throat diameters.« less

  2. Synchronous temperature rate control for refrigeration with reduced energy consumption

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gomes, Alberto Regio; Keres, Stephen L.; Kuehl, Steven J.

    Methods of operation for refrigerator appliance configurations with a controller, a condenser, at least one evaporator, a compressor, and two refrigeration compartments. The configuration may be equipped with a variable-speed or variable-capacity compressor, variable speed evaporator or compartment fans, a damper, and/or a dual-temperature evaporator with a valve system to control flow of refrigerant through one or more pressure reduction devices. The methods may include synchronizing alternating cycles of cooling each compartment to a temperature approximately equal to the compartment set point temperature by operation of the compressor, fans, damper and/or valve system. The methods may also include controlling themore » cooling rate in one or both compartments. Refrigeration compartment cooling may begin at an interval before or after when the freezer compartment reaches its lower threshold temperature. Freezer compartment cooling may begin at an interval before or after when the freezer compartment reaches its upper threshold temperature.« less

  3. Synchronous temperature rate control for refrigeration with reduced energy consumption

    DOEpatents

    Gomes, Alberto Regio; Keres, Stephen L.; Kuehl, Steven J.; Litch, Andrew D.; Richmond, Peter J.; Wu, Guolian

    2015-09-22

    Methods of operation for refrigerator appliance configurations with a controller, a condenser, at least one evaporator, a compressor, and two refrigeration compartments. The configuration may be equipped with a variable-speed or variable-capacity compressor, variable speed evaporator or compartment fans, a damper, and/or a dual-temperature evaporator with a valve system to control flow of refrigerant through one or more pressure reduction devices. The methods may include synchronizing alternating cycles of cooling each compartment to a temperature approximately equal to the compartment set point temperature by operation of the compressor, fans, damper and/or valve system. The methods may also include controlling the cooling rate in one or both compartments. Refrigeration compartment cooling may begin at an interval before or after when the freezer compartment reaches its lower threshold temperature. Freezer compartment cooling may begin at an interval before or after when the freezer compartment reaches its upper threshold temperature.

  4. Nutrition: blood sample collection

    NASA Image and Video Library

    2007-03-20

    ISS014-E-17550 (20 March 2007) --- Astronaut Michael E. Lopez-Alegria, Expedition 14 commander and NASA space station science officer, prepares to insert a test sample in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.

  5. Nutrition: blood sample collection

    NASA Image and Video Library

    2007-03-20

    ISS014-E-17547 (20 March 2007) --- Astronaut Michael E. Lopez-Alegria, Expedition 14 commander and NASA space station science officer, prepares to insert a test sample in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods.

  6. Medication and trial duration influence postural and pointing parameters during a standing repetitive pointing task in individuals with Parkinson's disease.

    PubMed

    Jehu, Deborah A; Cantù, Hiram; Hill, Allen; Paquette, Caroline; Côté, Julie N; Nantel, Julie

    2018-01-01

    We aimed to determine the effects of levodopa medication on the performance of a repetitive pointing task while standing, and to investigate the optimal trial duration in individuals with Parkinson's disease, and older adults. Seventeen individuals with Parkinson's disease (5 freezers) and 9 older adults stood on force platforms for 30 s and 120 s while performing a bilateral repetitive pointing task, tracked by motion capture. Participants with Parkinson's disease were assessed on and off medication and older adults were also assessed on separate days. The main findings were that: 1) on medication, participants with Parkinson's exhibited greater center of pressure root mean square in the medial-lateral direction, greater velocity in the medial-lateral and anterior-posterior directions, and greater range in the medial-lateral direction than off medication; 2) longer trial durations resulted in greater center of pressure range in the medial-lateral and anterior-posterior directions and greater coefficient of variation in finger pointing on the least affected side; 3) Parkinson's participants exhibited larger range in the medial-lateral direction compared to older adults; 4) off medication, freezers presented with less range and root mean square in the anterior-posterior direction than non-freezers; and 5) a correlation emerged between the freezing of gait questionnaire and pointing asymmetry and the coefficient of variation of pointing on the most affected side. Therefore, Parkinson's medication may increase instability during a repetitive pointing task. Longer trials may provide a better depiction of sway by discriminating between those with and without neurological impairment. Individuals with Parkinson's were less stable than older adults, supporting that they are at a greater risk for falls. The greater restrictive postural strategy in freezers compared to non-freezers is likely a factor that augments fall-risk. Lastly, the link between freezing of gait and upper-limb movement indicates that freezing may manifest first in the lower-limbs.

  7. Hydrophilic structures for condensation management in refrigerator appliances

    DOEpatents

    Kuehl, Steven John; Vonderhaar, John J; Wu, Guolian; Wu, Mianxue

    2014-10-21

    A refrigerator appliance that includes a freezer compartment having a freezer compartment door, and a refrigeration compartment having at least one refrigeration compartment door. The appliance further includes a mullion with an exterior surface. The mullion divides the compartments and the exterior surface directs condensation toward a transfer point. The appliance may also include a cabinet that houses the compartments and has two sides, each with an exterior surface. Further, at least one exterior surface directs condensation toward a transfer point.

  8. Effect of freezing method and frozen storage duration on lamb sensory quality.

    PubMed

    Muela, E; Sañudo, C; Campo, M M; Medel, I; Beltrán, J A

    2012-01-01

    This study assessed the effect of three freezing methods with three frozen storage durations (1, 3, and 6 months) on the sensory quality of lamb. Methods were: air blast freezer, freezing tunnel+air blast freezer, and nitrogen chamber+air blast freezer. Meat was frozen after 48 h of ageing (0-4°C). Fresh meat (72 h ageing at 2-4°C) was used as control. Sensory analyses (trained panel and consumer tests) were performed on loin chops (Longissimus lumborum) after 24 h of thawing. Results from the trained panel test showed that freezing (method and/or storage duration) had no significant effect. Consumers found that freezing affected sensory quality. Cluster analysis for overall acceptability divided the population into four classes with different preference patterns, and none of them showed a significant preference for fresh meat. The small differences between fresh and thawed meat shown in this study should not give consumers concerns about buying frozen meat or consuming thawed meat. Copyright © 2011. Published by Elsevier Ltd.

  9. Successful short-term cryopreservation of volume-reduced cord blood units in a cryogenic mechanical freezer: effects on cell recovery, viability, and clonogenic potential.

    PubMed

    Anagnostakis, Ioannis; Papassavas, Andreas C; Michalopoulos, Efstathios; Chatzistamatiou, Theofanis; Andriopoulou, Sofia; Tsakris, Athanassios; Stavropoulos-Giokas, Catherine

    2014-01-01

    Cord blood (CB) units are stored from weeks to years in liquid- or vapor-phase nitrogen until they are used for transplantation. We examined the effects of cryostorage in a mechanical freezer at -150°C on critical quality control variables of CB collections to investigate the possible use of mechanical freezers at -150°C as an alternative to storage in liquid- (or vapor-) phase nitrogen. A total of 105 CB units were thawed and washed at different time intervals (6, 12, 24, and 36 months). For every thawed CB unit, samples were removed and cell enumeration (total nucleated cells [TNCs], mononuclear cells [MNCs], CD34+, CD133+) was performed. In addition, viability was obtained with the use of flow cytometry, and recoveries were calculated. Also, total absolute colony-forming unit counts were performed and progenitor cell recoveries were studied by clonogenic assays. Significant differences (p < 0.05) were observed in certain variables (TNCs, MNC numbers, viability) when they were examined in relation with time intervals, while others (CD34+, CD133+) were relatively insensitive (p = NS) to the duration of time interval the CB units were kept in cryostorage condition. The data presented suggest that cryopreservation of CB units in a mechanical freezer at -150°C may represent an alternative cryostorage condition for CB cryopreservation. © 2013 American Association of Blood Banks.

  10. Is sperm freezability related to the post-thaw lipid peroxidation and the formation of reactive oxygen species in boars?

    PubMed

    Gómez-Fernández, J; Gómez-Izquierdo, E; Tomás, C; Mocé, E; de Mercado, E

    2013-04-01

    The aim of the present study was to determine whether the levels of reactive oxygen species (ROS) substances production and the levels of lipid peroxidation of the sperm membrane were related to the quality that the ejaculates exhibited after cryopreservation in boars. Ejaculates from 42 healthy boars were used in this study and they were cryopreserved with the lactose-egg yolk extender (LEY). Several sperm quality parameters were assessed by flow cytometry in samples incubated for 30 and 150 min at 37 °C after thawing: the percentage of sperm with intact plasma membrane (SIPM), intracellular reactive oxygen substances production through mean of DCF fluorescence intensity of total sperm (mean-DCF) and the percentage of viable and non-viable sperm containing oxidized BODIPY (VSOB and NVSOB). In addition, the percentages of total motile (TMS) and progressively motile sperm (PMS) were assessed at the same incubation times with a computer-assisted sperm analysis system. The classification of the ejaculates into good or bad freezers was performed through hierarchical cluster analysis from SIPM and TMS at 150 min post-thawing. The ejaculates of those males classified as good freezers exhibited higher (p < 0.05) SPIM, TMS and PMS than the bad freezers, although both groups presented similar (p > 0.05) VSOB, NVSOB and mean-DCF. Therefore, these results show that lipid peroxidation and the amount of reactive oxygen substances in the sperm after cryopreservation are similar between boars classified as good or bad freezers. © 2012 Blackwell Verlag GmbH.

  11. Efficient biostorage below -150 °C, without sacrificial cryogen

    NASA Astrophysics Data System (ADS)

    Spoor, Philip S.

    2017-12-01

    Biostorage is a multi-billion dollar business worldwide and growing rapidly; yet the commercially available options force the user to choose either optimal storage temperature (below -137 °C, the “glass transition temperature” of water) or convenience (no cryogen refill). Passive liquid-nitrogen freezers (storage Dewars with liquid nitrogen pooled at the bottom) provide very cold storage (-190 °C) but the LN2 must be replenished as it boils off. The alternative, so-called “ultra-low” vapor-compression freezers, have no cryogens to replenish and are convenient to use, but only reach storage temperatures above -90 °C. In addition, these tend to be inefficient and costly. Chart Industries is introducing a novel combination of a storage Dewar with a cryocooler (the “Fusion” freezer), that can maintain storage temperatures below -150 °C without the need to replenish any cryogen, while drawing less electricity than any ultra-low on the market. This new product also fits into a relatively narrow “demand window” where on-site cryocooling is not merely more convenient, but also more costeffective than liquid nitrogen delivery.

  12. Controlled rate cooling of fungi using a stirling cycle freezer.

    PubMed

    Ryan, Matthew J; Kasulyte-Creasey, Daiva; Kermode, Anthony; San, Shwe Phue; Buddie, Alan G

    2014-01-01

    The use of a Stirling cycle freezer for cryopreservation is considered to have significant advantages over traditional methodologies including N2 free operation, application of low cooling rates, reduction of sample contamination risks and control of ice nucleation. The study assesses the suitability of an 'N2-free' Stirling Cycle controlled rate freezer for fungi cryopreservation. In total, 77 fungi representing a broad taxonomic coverage were cooled using the N2 free cooler following a cooling rate of -1 degrees C min(-1). Of these, 15 strains were also cryopreserved using a traditional 'N2 gas chamber' controlled rate cooler and a comparison of culture morphology and genomic stability against non-cryopreserved starter cultures was undertaken. In total of 75 fungi survived cryopreservation, only a recalcitrant Basidiomycete and filamentous Chromist failed to survive. No changes were detected in genomic profile after preservation, suggesting that genomic function is not adversely compromised as a result of using 'N2 free' cooling. The results demonstrate the potential of 'N2-free' cooling for the routine cryopreservation of fungi in Biological Resource Centres.

  13. Effects of different freezing methods on calcium enriched papaya (Carica papaya L.).

    PubMed

    Lovera, Nancy N; Ramallo, Laura; Salvadori, Viviana O

    2018-06-01

    The effect of calcium impregnation on drip loss, colour, mechanical properties, sensory perception and freezing time on frozen-thawed papaya was studied, evaluating different freezing methods: cryogenic, tunnel and household freezer freezing. Osmotic dehydration as pre-treatment was also evaluated. Freezing in liquid nitrogen was considered an inappropriate method for papaya preservation due to cracking. Calcium impregnation and osmotic dehydration increased tissue firmness and decreased freezing time (freezing time for fresh, calcium impregnated and osmo-dehydrated fruit was 23, 17 and 5 min in a tunnel and 118, 83 and 60 min in a household freezer, respectively). Calcium lactate was the most effective way to protect tissue's firmness before and after a freeze-thaw cycle (maximum stress values approx. 300-400% of the raw tissue for tunnel freezing and 260% for household freezer). Microstructure analysis showed better tissue integrity retention in papaya samples impregnated with calcium lactate than in those with calcium gluconate, after a freezing-thawing cycle, in agreement with the drip loss results. In spite of these results, consumers preferred frozen papaya without pre-treatment or impregnated with calcium gluconate.

  14. Williams loads the MELFI for the Nutrition Experiment during Expedition 15

    NASA Image and Video Library

    2007-06-01

    ISS015-E-10572 (1 June 2007) --- Astronaut Sunita L. Williams, Expedition 15 flight engineer, inserts test samples in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as a part of the Nutritional Status Assessment (Nutrition) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

  15. Chamitoff works with the MELFI in the U.S. Laboratory during Expedition 17

    NASA Image and Video Library

    2008-09-27

    ISS017-E-017541 (27 Sept. 2008) --- NASA astronaut Greg Chamitoff, Expedition 17 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

  16. Chamitoff works with the MELFI in the U.S. Laboratory during Expedition 17

    NASA Image and Video Library

    2008-09-27

    ISS017-E-017539 (27 Sept. 2008) --- NASA astronaut Greg Chamitoff, Expedition 17 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

  17. Anderson works on the NUTRITION Experiment in the US Lab during Expedition 15

    NASA Image and Video Library

    2007-06-25

    ISS015-E-13670 (25 June 2007) --- Astronaut Clayton Anderson, Expedition 15 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

  18. Anderson works on the NUTRITION Experiment in the US Lab during Expedition 15

    NASA Image and Video Library

    2007-06-25

    ISS015-E-13695 (25 June 2007) --- Astronaut Clayton Anderson, Expedition 15 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) experiment in the Destiny laboratory of the International Space Station. MELFI is a low temperature freezer facility with nominal operating temperatures of -80, -26 and +4 degrees Celsius that will preserve experiment materials over long periods. The results of the Nutrition experiment will be used to better understand the time course effects of space flight on human physiology.

  19. User evaluation of photovoltaic-powered vaccine refrigerator/freezer systems

    NASA Technical Reports Server (NTRS)

    Ratajczak, Anthony F.

    1987-01-01

    The NASA Lewis Research Center has concluded a project to develop and field test photovoltaic-powered refrigerator/freezers for vaccine storage in remote areas of developing countries. As a conclusion to this project, questionnaires were sent to the in-country administrators for each test site probing user acceptance of the systems and attitudes regarding procurement of additional systems. Responses indicate that the systems had a positive effect on the local communities, that they made a positive impression on the local health authorities, and that system cost and scarcity of funds are the major barriers to procurements of additional systems.

  20. Solar photovoltaic powered refrigerators/freezers for medical use in remote geographic locations

    NASA Technical Reports Server (NTRS)

    Darkazalli, G.; Hein, G. F.

    1983-01-01

    One of the obstacles preventing widespread immunication against disease is the virtual absence of reliable, low maintenance refrigeration systems for storage of vaccines in remote geographic locations. A system which consists of a solar photovoltaic cell array and an integrated refrigerator/freezer-energy storage unit is discussed herein. The array converts solar radiation into direct current (DC) electricity with no moving parts and no intermediate steps. A detailed description of the refrigeration system, its design and an analysis thereof, performance test procedures, and test results are presented. A system schematic is also provided.

  1. User evaluation of photovoltaic-powered vaccine refrigerator/freezer systems

    NASA Astrophysics Data System (ADS)

    Ratajczak, Anthony F.

    1987-03-01

    The NASA Lewis Research Center has concluded a project to develop and field test photovoltaic-powered refrigerator/freezers for vaccine storage in remote areas of developing countries. As a conclusion to this project, questionnaires were sent to the in-country administrators for each test site probing user acceptance of the systems and attitudes regarding procurement of additional systems. Responses indicate that the systems had a positive effect on the local communities, that they made a positive impression on the local health authorities, and that system cost and scarcity of funds are the major barriers to procurements of additional systems.

  2. Production of bovine cloned embryos with donor cells frozen at a slow cooling rate in a conventional freezer (20 C)

    USGS Publications Warehouse

    Chacon, L.; Gomez, M.C.; Jenkins, J.A.; Leibo, S.P.; Wirtu, G.; Dresser, B.L.; Pope, C.E.

    2009-01-01

    Summary Usually, fibroblasts are frozen in dimethyl sulphoxide (DMSO, 10% v/v) at a cooling rate of 1 C/min in a low-temperature (80 C) freezer (LTF) before storage in liquid nitrogen (LN2); however, a LTF is not always available. The purpose of the present study was to evaluate apoptosis and viability of bovine fibroblasts frozen in a LTF or conventional freezer (CF; 20 C) and their subsequent ability for development to blastocyst stage after fusion with enucleated bovine oocytes. Percentages of live cells frozen in LTF (49.5%) and CF (50.6%) were similar, but significantly less than non-frozen control (88%). In both CF and LTF, percentages of live apoptotic cells exposed to LN2 after freezing were lower (4% and 5%, respectively) as compared with unexposed cells (10% and 18%, respectively). Cells frozen in a CF had fewer cell doublings/24 h (0.45) and required more days (9.1) to reach 100% confluence at the first passage (P) after thawing and plating as compared with cells frozen in a LTF (0.96 and 4.0 days, respectively). Hypoploidy at P12 was higher than at P4 in cells frozen in either a CF (37.5% vs. 19.2%) or in a LTF (30.0% vs. 15.4%). A second-generation cryo-solution reduced the incidence of necrosis (29.4%) at 0 h after thawing as compared with that of a first generation cryo-solution (DMEM + DMSO, 60.2%). The percentage of apoptosis in live cells was affected by cooling rate (CF = 1.9% vs. LFT = 0.7%). Development of bovine cloned embryos to the blastocyst stage was not affected by cooling rate or freezer type. ?? 2009 Cambridge University Press.

  3. Improvement of common carp (Cyprinus carpio) sperm cryopreservation using a programable freezer.

    PubMed

    Bernáth, Gergely; Żarski, Daniel; Kása, Eszter; Staszny, Ádám; Várkonyi, Levente; Kollár, Tímea; Hegyi, Árpád; Bokor, Zoltán; Urbányi, Béla; Horváth, Ákos

    2016-10-01

    The applicability of a programmable freezer for the increased-scale cryopreservation of common carp sperm was investigated. The effect of different equilibration times, cryopreservation methods, extenders, dilution ratios, activating solutions on the post-thaw motility of common carp sperm was investigated. The suitable post-thaw storage time-interval as well as fertilizing capacity of cryopreserved sperm was also examined. The motility, curvilinear velocity (VCL) and straightness (STR) values did not decrease significantly during 60min of equilibration neither in equilibrated nor thawed groups. Motility parameters of thawed sperm were similar using a conventional cryopreservation technique using a polystyrene box [motility (33%), VCL (47μm/s) and STR (88%)] and a programmable freezer: [motility (32%), VCL (54μm/s) and STR (89%)]. The highest motility and VCL was measured with a sugar based extender (grayling extender) at a ratio 1:9 (motility: 52%, VCL: 76μm/s) and 1:20 (motility: 49%, VCL: 76μm/s). The activating solution for cyprinids (ASC) could prolong sperm movement up for 2min. A storage time of six hours following thawing did not have a significant effect on the motility parameters of thawed carp sperm. Agglutination was observed during cryopreservation of an elevated volume of sperm whereas motility 47%, VCL 62μm/s and STR 91% were measured after thawing. Fertilization rate with thawed sperm (32%) was significantly lower compared to the control group (73%). According to our results, the developed method using a programmable freezer is suitable for the cryopreservation of elevated number of straws. However, carp sperm agglutination during freezing may have a negative effect on the fertilizing capacity. Copyright © 2016 Elsevier Inc. All rights reserved.

  4. Changes in electrical energy requirements to operate an ice cream freezer as a function of sweeteners and gums

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Smith, D.E.; Bakshi, A.S.; Gay, S.A.

    1985-01-01

    Changes in electrical energy required to operate a continuous freezer were monitored for various ice cream formulae. Ice cream formulae consisted of nine different combinations of sucrose, 36 DE corn syrup, and 42 high fructose corn syrup as well as two ratios of guar gum to locust bean gum. Within the same sweetening system, a mix high in locust bean gum tended to have a lower energy demand than mix with large amounts of guar gum. This was especially pronounced in mixes with 50% 42 high fructose corn syrup and/or 50% 36 DE corn syrup solids.

  5. The ESA Laboratory Support Equipment for the ISS.

    PubMed

    Petrivelli, A

    2002-02-01

    The Laboratory Support Equipment (LSE) for the International Space Station (ISS) is a suite of general-purpose items that will be available onboard the Station either as self-standing facilities or as equipment that can be used at defined locations. Dedicated to supporting system maintenance and payload operations, some LSE items are derived from commercial equipment, while others have been specifically developed for the ISS. ESA is currently engaged in developing three pressurised facilities and one pointing mechanism that will become part of the LSE complement, namely: the Minus Eighty degree centigrade Laboratory Freezer for the ISS (MELFI), the Microgravity Science Glovebox (MSG), the cryogenic storage and quick/snap freezer system (Cryosystem), the external-payload pointing system (Hexapod).

  6. Is employer coverage of elective egg freezing coercive?: a survey of medical students' knowledge, intentions, and attitudes towards elective egg freezing and employer coverage.

    PubMed

    Ikhena-Abel, Deborah E; Confino, Rafael; Shah, Nirali J; Lawson, Angela K; Klock, Susan C; Robins, Jared C; Pavone, Mary Ellen

    2017-08-01

    The purpose of this study was to understand medical students' knowledge, intentions, and attitudes towards oocyte cryopreservation and employer coverage of such treatment. This cross-sectional study was performed via an online cross-sectional survey distributed to 280 female medical students from March through August 2016. Demographics, attitudes towards employer coverage, and factors influencing decision-making were assessed via a self-reported multiple-choice questionnaire. The relationship between respondents' attitudes towards employer coverage and other parameters was analyzed. A total of 99 responses were obtained out of 280 female medical students. Most respondents (71%) would consider oocyte cryopreservation (potential freezers), although 8% would not consider the procedure and 21% were unsure. Seventy-six percent of respondents felt pressure to delay childbearing. Potential freezers were more likely to be single (p = 0.001), to report feeling pressure to delay childbearing (p = 0.016), and to consider egg freezing if offered by an employer (p < 0.001). Importantly, 71% percent did not view employer coverage as coercive and 77% of respondents would not delay childbearing due to employer coverage. Factors influencing decision-making in potential freezers were absence of a suitable partner (83%), likelihood of success (95%), and health of offspring (94%), among others. Knowledge about the low chance of pregnancy per oocyte (6-10%) would influence decision-making in 42% of potential freezers. Oocyte freezing is an acceptable strategy for the majority of young women surveyed. Pressure to delay childbearing was related to openness to freeze eggs. The majority of respondents did not find employer coverage for egg freezing coercive although further research is needed with larger, representative samples to ascertain the relationship between pressure to delay childbearing due to work demands and employer coverage for egg freezing.

  7. Advanced Refrigerator/Freezer Technology Development. Technology Assessment

    NASA Technical Reports Server (NTRS)

    Gaseor, Thomas; Hunter, Rick; Hamill, Doris

    1996-01-01

    The NASA Lewis Research Center, through contract with Oceaneering Space Systems, is engaged in a project to develop advanced refrigerator/freezer (R/F) technologies for future Life and Biomedical Sciences space flight missions. The first phase of this project, a technology assessment, has been completed to identify the advanced R/F technologies needed and best suited to meet the requirements for the five R/F classifications specified by Life and Biomedical Science researchers. Additional objectives of the technology assessment were to rank those technologies based on benefit and risk, and to recommend technology development activities that can be accomplished within this project. This report presents the basis, the methodology, and results of the R/F technology assessment, along with technology development recommendations.

  8. MELFI ops

    NASA Image and Video Library

    2013-06-06

    Astronaut Karen Nyberg,Expedition 36 flight engineer,works with samples in Minus Eighty-Degree Laboratory Freezer for ISS (MELFI-3) in the Destiny laboratory of the Earth-orbiting International Space Station.

  9. 15 CFR 9.3 - Appliances and equipment included in program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... and central air conditioners, household refrigerators, home freezers, clothes washers, dishwashers, clothes dryers, kitchen ranges and ovens, water heaters, comfort heating equipment, and television...

  10. 15 CFR 9.3 - Appliances and equipment included in program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and central air conditioners, household refrigerators, home freezers, clothes washers, dishwashers, clothes dryers, kitchen ranges and ovens, water heaters, comfort heating equipment, and television...

  11. 15 CFR 9.3 - Appliances and equipment included in program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... and central air conditioners, household refrigerators, home freezers, clothes washers, dishwashers, clothes dryers, kitchen ranges and ovens, water heaters, comfort heating equipment, and television...

  12. 15 CFR 9.3 - Appliances and equipment included in program.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... and central air conditioners, household refrigerators, home freezers, clothes washers, dishwashers, clothes dryers, kitchen ranges and ovens, water heaters, comfort heating equipment, and television...

  13. 15 CFR 9.3 - Appliances and equipment included in program.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and central air conditioners, household refrigerators, home freezers, clothes washers, dishwashers, clothes dryers, kitchen ranges and ovens, water heaters, comfort heating equipment, and television...

  14. 48 CFR 23.804 - Contract clauses.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... refrigerant, such as air conditioners, including motor vehicles, refrigerators, chillers, or freezers. [61 FR... Equipment and Air Conditioners, in solicitations and contracts for services when the contract includes the...

  15. Qualification testing of solar photovoltaic powered refrigerator freezers for medical use in remote geographic locations

    NASA Astrophysics Data System (ADS)

    Kaszeta, W. J.

    1982-12-01

    One of the primary obstacles to the application of vaccination in developing countries is the lack of refrigerated storage. Vaccines exposed to elevated temperatures suffer a permanent loss of potency. Photovoltaic (PV) powered refrigerator/freezer (R/F) units could surmount the problem of refrigeration in remote areas where no reliable commercial power supply is available. The performance measurements of two different models of PV powered R/F units for medical use are presented. Qualification testing consisted of four major procedures: no-load pull down, ice making, steady-state (maintenance), and holdover. Both R/F units met the major World Health Organization (WHO) requirements. However, the testing performed does not provide complete characterization of the two units; such information could be derived only from further extensive test procedures.

  16. Qualification testing of solar photovoltaic powered refrigerator freezers for medical use in remote geographic locations

    NASA Technical Reports Server (NTRS)

    Kaszeta, W. J.

    1982-01-01

    One of the primary obstacles to the application of vaccination in developing countries is the lack of refrigerated storage. Vaccines exposed to elevated temperatures suffer a permanent loss of potency. Photovoltaic (PV) powered refrigerator/freezer (R/F) units could surmount the problem of refrigeration in remote areas where no reliable commercial power supply is available. The performance measurements of two different models of PV powered R/F units for medical use are presented. Qualification testing consisted of four major procedures: no-load pull down, ice making, steady-state (maintenance), and holdover. Both R/F units met the major World Health Organization (WHO) requirements. However, the testing performed does not provide complete characterization of the two units; such information could be derived only from further extensive test procedures.

  17. Activation of orexin/hypocretin neurons is associated with individual differences in cued fear extinction.

    PubMed

    Sharko, Amanda C; Fadel, Jim R; Kaigler, Kris F; Wilson, Marlene A

    2017-09-01

    Identifying the neurobiological mechanisms that underlie differential sensitivity to stress is critical for understanding the development and expression of stress-induced disorders, such as post-traumatic stress disorder (PTSD). Preclinical studies have suggested that rodents display different phenotypes associated with extinction of Pavlovian conditioned fear responses, with some rodent populations being resistant to extinction. An emerging literature also suggests a role for orexins in the consolidation processes associated with fear learning and extinction. To examine the possibility that the orexin system might be involved in individual differences in fear extinction, we used a Pavlovian conditioning paradigm in outbred Long-Evans rats. Rats showed significant variability in the extinction of cue-conditioned freezing and extinction recall, and animals were divided into groups based on their extinction profiles based on a median split of percent freezing behavior during repeated exposure to the conditioned cue. Animals resistant to extinction (high freezers) showed more freezing during repeated cue presentations during the within trial and between trial extinction sessions compared with the group showing significant extinction (low freezers), although there were no differences between these groups in freezing upon return to the conditioned context or during the conditioning session. Following the extinction recall session, activation of orexin neurons was determined using dual label immunohistochemistry for cFos in orexin positive neurons in the hypothalamus. Individual differences in the extinction of cue conditioned fear were associated with differential activation of hypothalamic orexin neurons. Animals showing poor extinction of cue-induced freezing (high freezers) had significantly greater percentage of orexin neurons with Fos in the medial hypothalamus than animals displaying significant extinction and good extinction recall (low freezers). Further, the freezing during extinction learning was positively correlated with the percentage of activated orexin neurons in both the lateral and medial hypothalamic regions. No differences in the overall density of orexin neurons or Fos activation were seen between extinction phenotypes. Although correlative, our results support other studies implicating a role of the orexinergic system in regulating extinction of conditioned responses to threat. Copyright © 2016 Elsevier Inc. All rights reserved.

  18. 29 CFR 784.135 - “Packing.”

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... with other boxes, loading into a quick freezer locker, removing after freezing, emptying the box... cartons and finally stowing in refrigerated locker. General Character and Scope of the Section 13(a)(4...

  19. 29 CFR 784.135 - “Packing.”

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... with other boxes, loading into a quick freezer locker, removing after freezing, emptying the box... cartons and finally stowing in refrigerated locker. General Character and Scope of the Section 13(a)(4...

  20. 29 CFR 784.135 - “Packing.”

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... with other boxes, loading into a quick freezer locker, removing after freezing, emptying the box... cartons and finally stowing in refrigerated locker. General Character and Scope of the Section 13(a)(4...

  1. 29 CFR 784.135 - “Packing.”

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... with other boxes, loading into a quick freezer locker, removing after freezing, emptying the box... cartons and finally stowing in refrigerated locker. General Character and Scope of the Section 13(a)(4...

  2. 29 CFR 784.135 - “Packing.”

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... with other boxes, loading into a quick freezer locker, removing after freezing, emptying the box... cartons and finally stowing in refrigerated locker. General Character and Scope of the Section 13(a)(4...

  3. Commander Lousma works with EEVT experiment and cryogenic tube on aft middeck

    NASA Image and Video Library

    1982-03-31

    Commander Jack Lousma works with Electrophoresis Equipment Verification Test (EEVT) electrophoresis unit, cryogenic freezer and tube, and stowage locker equipment located on crew compartment middeck aft bulkhead.

  4. Creamer stows urine samples in MELFI

    NASA Image and Video Library

    2010-05-02

    ISS023-E-030773 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  5. Creamer stows urine samples in MELFI

    NASA Image and Video Library

    2010-05-02

    ISS023-E-030771 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  6. Creamer stows urine samples in MELFI

    NASA Image and Video Library

    2010-05-02

    ISS023-E-030780 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  7. Creamer stows urine samples in MELFI

    NASA Image and Video Library

    2010-05-02

    ISS023-E-030772 (2 May 2010) --- NASA astronaut T.J. Creamer, Expedition 23 flight engineer, services the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  8. 87. AFT CREWS' MESS DECK STARBOARD LOOKING TO PORT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    87. AFT CREWS' MESS DECK - STARBOARD LOOKING TO PORT SHOWING COFFEE MAKER, ICE CREAM FREEZER, TABLES AND SCUTTLEBUTTS. - U.S.S. HORNET, Puget Sound Naval Shipyard, Sinclair Inlet, Bremerton, Kitsap County, WA

  9. Experimental Investigation of Effectiveness of Magnetic Field on Food Freezing Process

    NASA Astrophysics Data System (ADS)

    Suzuki, Toru; Takeuchi, Yuri; Masuda, Kazunori; Watanabe, Manabu; Shirakashi, Ryo; Fukuda, Yutaka; Tsuruta, Takaharu; Yamamoto, Kazutaka; Koga, Nobumitsu; Hiruma, Naoya; Ichioka, Jun; Takai, Kiyoshi

    Recently, several food refrigeration equipments that utilize magnetic field have attracted much attention from food production companies, consumers and mass media. However, the effectiveness of the freezers is not scientifically examined. Therefore, the effectiveness should be clarified by experiments or theoretical considerations. In this study, the effect of weak magnetic field (about 0.0005 T) on freezing process of several kinds of foods was investigated by using a specially designed freezer facilitated with magnetic field generator. The investigation included the comparison of freezing curves, drip amount, physicochemical evaluations on color and texture, observation of microstructure, and sensory evaluation. From the results of the control experiments, it can be concluded that weak magnetic field around 0.0005 T provided no significant difference on temperature history during freezing and on the qualities of frozen foods, within our experimental conditions.

  10. Staged regenerative sorption heat pump

    NASA Technical Reports Server (NTRS)

    Jones, Jack A. (Inventor)

    1995-01-01

    A regenerative adsorbent heat pump process and system for cooling and heating a space. A sorbent is confined in a plurality of compressors of which at least four are first stage and at least four are second stage. The first stage operates over a first pressure region and the second stage over a second pressure region which is higher than the first. Sorbate from the first stage enters the second stage. The sorbate loop includes a condenser, expansion valve, evaporator and the compressors. A single sorbate loop can be employed for single-temperature-control such as air conditioning and heating. Two sorbate loops can be used for two-temperature-control as in a refrigerator and freezer. The evaporator temperatures control the freezer and refrigerator temperatures. Alternatively the refrigerator temperature can be cooled by the freezer with one sorbate loop. A heat transfer fluid is circulated in a closed loop which includes a radiator and the compressors. Low temperature heat is exhausted by the radiator. High temperature heat is added to the heat transfer fluid entering the compressors which are desorbing vapor. Heat is transferred from compressors which are sorbing vapor to the heat transfer fluid, and from the heat transfer fluid to the compressors which are desorbing vapor. Each compressor is subjected to the following phases, heating to its highest temperature, cooling down from its highest temperature, cooling to its lowest temperature, and warming up from its lowest temperature. The phases are repeated to complete a cycle and regenerate heat.

  11. Aircraft loading and freezer enhancements: lessons for medical research in remote communities.

    PubMed

    Gagnon, Roy; Gagnon, Faith; Panagiotopoulos, Constadina

    2008-01-01

    Type 2 diabetes (T2D) and impaired glucose tolerance (IGT), historically extremely rare in children, is becoming prevalent among First Nations children. In Canada, many of these children live in remote villages accessible only by float plane. Because T2D has many long-term health implications, prevention and early identification are critical. We developed a process for sending a fully equipped endocrinology team to a remote community to screen the children for T2D and IGT. Float plane (sea plane) travel has several unexpected limitations for a medical research team. These include having to travel in good visibility (visual flight rules), limited payload capacity, and restriction against transporting dry ice. The benefits include avoiding the usual security restrictions. We developed and tested a custom-built insulation jacket and system of backup battery packs for the countertop -25 degrees C freezer (in lieu of dry ice) to transport frozen blood samples from the village to our hospital's laboratory. We also ensured that the five-member research team, its equipment, and the consumable supplies stayed within the maximum takeoff weight of the airplane and met center-of-gravity criteria to ensure a safe flight. Using the insulated freezer, sample integrity was maintained throughout the flight, and a safe weight-and-balance trip was achieved for the team and supplies. The team obtained complete T2D screening data on 88% of children in the remote community.

  12. Effect of Freezing Rate and Microwave Thawing on Texture and Microstructural Properties of Potato (Solanum tuberosum).

    PubMed

    Phinney, David M; Frelka, John C; Wickramasinghe, Anita; Heldman, Dennis R

    2017-04-01

    Food freezing is a preservation process that works by lowering temperature while simultaneously decreasing water activity. It is accepted that although freezing preserves foods, it generally has a negative effect on textural quality. This research investigated the texture response of potatoes (Solanum tuberosum) as a function of time to freeze (defined as the time for the center temperature to reach -20 °C) and thawing process. Potatoes slices (6 mm) were blanched then frozen in an ethanol/carbon dioxide bath, a pilot scale high velocity air freezer (HVAF) and a still air freezer to achieve various times to freeze. Slices were stabilized at -20 °C and thawed by 2 methods; room temperature air and microwave. Afterwards, samples were allowed to come to room temperature prior to texture profile analysis (TPA). Results indicate a maximum texture loss of the potato was reached at a time to freeze of approximately 8 min (corresponding to the HVAF). The texture difference between room temperature and microwave thawing methods was not shown to be significant (P = 0.05). SEM images showed the cellular structure of the potato in a HVAF to be similar to that of the still air freezer, validating that the matrix was maximally damaged in both conditions. This work created a continuous quality loss model for the potato as a function of time to freeze and showed no textural benefit to high velocity over still air freezing. © 2017 Institute of Food Technologists®.

  13. 7 CFR 58.624 - Freezers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS INSPECTION ACT (CONTINUED) GRADING AND INSPECTION...

  14. 7 CFR 58.624 - Freezers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS INSPECTION ACT (CONTINUED) GRADING AND INSPECTION...

  15. 76 FR 59394 - Energy Conservation Program for Consumer Products: Publication of the Extension of Interim Waiver...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-26

    ... Electronics America, Inc. From the Department of Energy Residential Refrigerator and Refrigerator-Freezer Test... Electronics America, Inc. (Samsung) from energy efficiency test procedure requirements that are applicable to...

  16. Lisa Smith in MSFC's Laboratory Training Complex

    NASA Image and Video Library

    2015-02-11

    LISA SMITH, THE TRAINING TEAM LEAD IN MARSHALL'S MISSION OPERATIONS LAB, EXAMINES THE DRAWERS IN THE GLACIER MOCK-UP, A TRAINING VERSION OF A FREEZER ON THE INTERNATIONAL SPACE STATION INSTALLED IN THE MARSHALL CENTER'S LABORATORY TRAINING COMPLEX

  17. Freezer anthropology: new uses for old blood.

    PubMed Central

    Merriwether, D A

    1999-01-01

    Archived blood fractions (plasma, settled red cells, white cells) have proved to be a rich and valuable source of DNA for human genetic studies. Large numbers of such samples were collected between 1960 and the present for protein and blood group studies, many of which are languishing in freezers or have already been discarded. More are discarded each year because the usefulness of these samples is not widely understood. Data from DNA derived from 10-35-year-old blood samples have been used to address the peopling of the New World and of the Pacific. Mitochondrial DNA haplotypes from studies using this source DNA support a single wave of migration into the New World (or a single source population for the New World), and that Mongolia was the likely source of the founding population. Data from Melanesia have shown that Polynesians are recent immigrants into the Pacific and did not arise from Melanesia. PMID:10091252

  18. Ice recrystallization inhibition in ice cream by propylene glycol monostearate.

    PubMed

    Aleong, J M; Frochot, S; Goff, H D

    2008-11-01

    The effectiveness of propylene glycol monostearate (PGMS) to inhibit ice recrystallization was evaluated in ice cream and frozen sucrose solutions. PGMS (0.3%) dramatically reduced ice crystal sizes in ice cream and in sucrose solutions frozen in a scraped-surface freezer before and after heat shock, but had no effect in quiescently frozen solutions. PGMS showed limited emulsifier properties by promoting smaller fat globule size distributions and enhanced partial coalescence in the mix and ice cream, respectively, but at a much lower level compared to conventional ice cream emulsifier. Low temperature scanning electron microscopy revealed highly irregular crystal morphology in both ice cream and sucrose solutions frozen in a scraped-surface freezer. There was strong evidence to suggest that PGMS directly interacts with ice crystals and interferes with normal surface propagation. Shear during freezing may be required for its distribution around the ice and sufficient surface coverage.

  19. Resurrection of a bull by cloning from organs frozen without cryoprotectant in a -80 degrees c freezer for a decade.

    PubMed

    Hoshino, Yoichiro; Hayashi, Noboru; Taniguchi, Shunji; Kobayashi, Naohiko; Sakai, Kenji; Otani, Tsuyoshi; Iritani, Akira; Saeki, Kazuhiro

    2009-01-01

    Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT). We report the cloning of a bull using cells retrieved from testicles that had been taken from a dead animal and frozen without cryoprotectant in a -80 degrees C freezer for 10 years. We obtained live cells from defrosted pieces of the spermatic cords of frozen testicles. The cells proliferated actively in culture and were apparently normal. We transferred 16 SCNT embryos from these cells into 16 synchronized recipient animals. We obtained five pregnancies and four cloned calves developed to term. Our results indicate that complete genome sets are maintained in mammalian organs even after long-term frozen-storage without cryoprotectant, and that live clones can be produced from the recovered cells.

  20. Effectiveness of measures to prevent unintentional deaths of infants and children from suffocation and strangulation.

    PubMed Central

    Kraus, J F

    1985-01-01

    Unintentional deaths from suffocation and strangulation account for about 20 percent of all nontransport-related infant and child fatalities in the United States. In the late 1950s, some preventive countermeasures were introduced to reduce the number of deaths resulting from refrigerator or freezer entrapment. A few years later, countermeasures were introduced to prevent deaths resulting from suffocation by plastic bags, inhumation, and mechanical strangulation from wedging in infant cribs. For three of these major causes of suffocation and strangulation deaths among infants and children (refrigerator or freezer entrapment, suffocation by plastic bag, and inhumation at construction sites), there appears to have been a significant decline in incidence; however, there is no evidence of a significant reduction in deaths from mechanical strangulation in cribs. The impact of current countermeasures is discussed, and some suggestions for new or modified approaches are made. PMID:3920722

  1. Cariogenic potential of stored human milk--an in-vitro study.

    PubMed

    Hegde, Amitha M; Vikyath, Rani

    2007-01-01

    Human milk samples collected from ten lactating mothers in the K. S. Hegde Medical Hospital, Mangalore were divided into five different parts and stored at different temperatures for varying durations. The pH, buffer capacity and growth of Streptococcus mutans were assessed in each of these samples. There was a fall in pH of human milk stored at various temperatures. The buffer capacity of human milk increased with duration of storage. There was an increase in Streptococcus colony count in stored human milk proportional to the duration of storage and it increased more rapidly in case of milk stored at higher temperatures (0 degrees C - 4 degrees C) compared to the milk stored in the freezer (-19 degrees C). Milk samples stored at room temperature for 6 hours and in the freezer at -19 degrees C for 2 weeks were found to be relatively safe.

  2. Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a −80°C Freezer for a Decade

    PubMed Central

    Hoshino, Yoichiro; Hayashi, Noboru; Taniguchi, Shunji; Kobayashi, Naohiko; Sakai, Kenji; Otani, Tsuyoshi; Iritani, Akira; Saeki, Kazuhiro

    2009-01-01

    Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT). We report the cloning of a bull using cells retrieved from testicles that had been taken from a dead animal and frozen without cryoprotectant in a −80°C freezer for 10 years. We obtained live cells from defrosted pieces of the spermatic cords of frozen testicles. The cells proliferated actively in culture and were apparently normal. We transferred 16 SCNT embryos from these cells into 16 synchronized recipient animals. We obtained five pregnancies and four cloned calves developed to term. Our results indicate that complete genome sets are maintained in mammalian organs even after long-term frozen-storage without cryoprotectant, and that live clones can be produced from the recovered cells. PMID:19129919

  3. Helium-Based Soundwave Chiller: Trillium: A Helium-Based Sonic Chiller- Tons of Freezing with 0 GWP Refrigerants

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    2010-09-01

    BEETIT Project: Penn State is designing a freezer that substitutes the use of sound waves and environmentally benign refrigerant for synthetic refrigerants found in conventional freezers. Called a thermoacoustic chiller, the technology is based on the fact that the pressure oscillations in a sound wave result in temperature changes. Areas of higher pressure raise temperatures and areas of low pressure decrease temperatures. By carefully arranging a series of heat exchangers in a sound field, the chiller is able to isolate the hot and cold regions of the sound waves. Penn State’s chiller uses helium gas to replace synthetic refrigerants. Becausemore » helium does not burn, explode or combine with other chemicals, it is an environmentally-friendly alternative to other polluting refrigerants. Penn State is working to apply this technology on a large scale.« less

  4. Influence of the freezing method on the changes that occur in grape samples after frozen storage.

    PubMed

    Santesteban, Luis G; Miranda, Carlos; Royo, José B

    2013-09-01

    Sample freezing is frequently used in oenological laboratories as a compromise solution to increase the number of samples that can be analysed, despite the fact that some grape characteristics are known to change after frozen storage. However, freezing is usually performed using standard freezers, which provide a slow freezing. The aim of this work was to evaluate whether blast freezing would decrease the impact of standard freezing on grape composition. Grape quality parameters were assessed in fresh and in frozen stored samples that had been frozen using three different procedures: standard freezing and blast freezing using either a blast freezer or an ultra-freezer. The implications of frozen storage in grape samples reported in earlier research were observed for the three freezing methods evaluated. Although blast freezing improved repeatability for the most problematic parameters (tartaric acidity, TarA; total phenolics, TP), the improvement was not important from a practical point of view. However, TarA and TP were relatively repeatable among the three freezing procedures, which suggests that freezing had an effect on these parameters independently of the method used . According to our results, the salification potential of the must is probably implied in the changes observed for TarA, whereas for TP the precipitation of protoanthocyanins after association with cell wall material is hypothesized to cause the lack of repeatability between fresh and frozen grapes. Blast freezing would not imply a great improvement if implemented in oenological laboratories, at least for the parameters included in this study. © 2013 Society of Chemical Industry.

  5. The Effects of a Secondary Task on Forward and Backward Walking in Parkinson Disease

    PubMed Central

    Hackney, Madeleine E.; Earhart, Gammon M.

    2009-01-01

    Background People with Parkinson disease (PD) often fall while multi-tasking or walking backward, unavoidable activities in daily living. Dual tasks involving cognitive demand during gait and unfamiliar motor skills like backward walking could identify those with fall risk, but dual tasking while walking backward has not been examined in those with PD, those who experience Freezing of Gait (FOG), or healthy older controls. Methods Seventy-eight people with PD (mean age = 65.1±9.5 years, Female: 28%) and 74 age- and sex-matched controls (mean age = 65.0±10.0 years, Female: 23%) participated. A computerized walkway measured gait velocity, stride length, swing and stance percent, cadence, heel to heel base of support, functional ambulation profile, and gait asymmetry during forward and backward walking with and without a secondary cognitive task. Results Direction and task effects on walking performance were similar between healthy controls and those with PD. However, those with PD were more affected than controls, and freezers were more affected than non-freezers, by backward walking and dual tasking. Walking backward seemed to impact gait more than dual tasking in those with PD, although the subset of freezers appeared particularly impacted by both challenges. Conclusion People with PD are impaired while performing complex motor and mental tasks simultaneously, which may put them at risk for falling. Those with FOG are more adversely affected by both motor and mental challenges than those without. Evaluation of backward walking while performing a secondary task might be an effective clinical tool to identify locomotor difficulties. PMID:19675121

  6. Effect of Aging and Freezing Conditions on Meat Quality and Storage Stability of 1++ Grade Hanwoo Steer Beef: Implications for Shelf Life

    PubMed Central

    Chang, Sunsik; Park, Beomyoung

    2017-01-01

    This study was conducted to establish the shelf life of 1++ grade Hanwoo beef by evaluating the changes in meat quality and storage stability under distribution conditions similar to those during export to Hong Kong and China. Four muscles of the loin, striploin, tenderloin, and top round muscles were obtained from 10 animals of 1++ grade Hanwoo steers. The distribution conditions were 0, 7, or 14 d of aging at 2°C and continuous storage at −18°C for 0, 3, 6, or 9 mon. The lightness (CIE L*) values decreased as the duration of freezer storage increased (p<0.05). The water-holding capacity of 4 muscles increased as the aging time increased when they were frozen for 3 mon (p<0.05). The cooking loss values of the four muscles were significantly increased as the duration of freezer storage increased (p<0.05). The Warner-Bratzler shear force values were significantly decreased in the loin, striploin, and top round muscles as the aging time increased (p<0.05). The changes in volatile basic nitrogen (16.67-18.49 mg%) and thiobarbituric reactive substance values (0.75-0.82 mg MA/kg meat) were significantly increased when the meat was frozen for 9 mon after 14 d of aging. On the basis of these observations, the shelf life of 1++ grade Hanwoo beef during distribution should be limited to less than 9 mon of freezer storage at −18°C after 14 d of aging at 2°C. PMID:28747830

  7. Pregnancy Cravings Can Harm Your Oral Health

    MedlinePlus

    ... have cravings for ice, freezer frost, or even soil. Pica combined with bulimia can have adverse effects ... Clothes •Ice •Paper •Plants •Cornstarch •Soap •Ashes •Paint •Soil Reviewed: January 2012 Related Articles: Expecting? Don’t ...

  8. Vascular Blood Collection stowage in MELFI

    NASA Image and Video Library

    2010-08-26

    ISS024-E-012555 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, replaces a dewar tray containing biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  9. Vascular Blood Collection stowage in MELFI

    NASA Image and Video Library

    2010-08-26

    ISS024-E-012553 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, replaces a dewar tray containing biological samples in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  10. Middle School Science Notes.

    ERIC Educational Resources Information Center

    School Science Review, 1979

    1979-01-01

    Included is information regarding: student construction of Newton's Discs to demonstrate color and of paper helicopters to encourage inquiry; improvising an apparatus demonstrating pressure between two bubbles; animal behavior experiments involving woodlice; two experiments using electronics freezer spray; chromatography of dyes; leaf keys; an…

  11. 8. FIRST FLOOR INTERIOR, ONE OF THREE MAIN REFRIGERATIONFREEZER ROOMS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. FIRST FLOOR INTERIOR, ONE OF THREE MAIN REFRIGERATION-FREEZER ROOMS (NO. 4), FROM CENTRAL CORRIDOR OPPOSITE ENTRANCE, LOOKING WEST. - Oakland Naval Supply Center, Cold Storage Warehouse, South of C Street between First & Second Street, Oakland, Alameda County, CA

  12. An Easy Classroom Experiment on the Supercooling of Water

    ERIC Educational Resources Information Center

    Gianino, Concetto

    2007-01-01

    The change from the state of supercooling to the solid state of ice is called superfreezing. This process is not uncommon and takes place in domestic freezers. It is also easy to reproduce in the laboratory. (Contains 6 figures.)

  13. 78 FR 54629 - Fisheries of the Exclusive Economic Zone Off Alaska; American Fisheries Act, Amendment 80 Program...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... Alaska Community Development Quota (CDQ) Program, and the hook-and-line catcher/processor (freezer... plans to present a draft analysis of the potential effects of cost recovery fee programs to the Council...

  14. MELFI-3 Electronic Unit Replacement

    NASA Image and Video Library

    2011-07-01

    ISS028-E-013799 (1 July 2011) --- NASA astronaut Mike Fossum, Expedition 28 flight engineer, replaces a failed electronic unit for the Minus Eighty Laboratory Freezer for ISS 3 (MELFI-3) with a spare unit in the Kibo laboratory of the International Space Station.

  15. MELFI-3 Electronic Unit Replacement

    NASA Image and Video Library

    2011-07-01

    ISS028-E-013797 (1 July 2011) --- NASA astronaut Mike Fossum, Expedition 28 flight engineer, replaces a failed electronic unit for the Minus Eighty Laboratory Freezer for ISS 3 (MELFI-3) with a spare unit in the Kibo laboratory of the International Space Station.

  16. An Experiment Using Sucrose Density Gradients in the Undergraduate Biochemistry Laboratory.

    ERIC Educational Resources Information Center

    Turchi, Sandra L.; Weiss, Monica

    1988-01-01

    Describes an experiment to be performed in an undergraduate biochemistry laboratory that is based on a gradient centrifugation system employing a simple bench top centrifuge, a freezer, and frozen surcose gradient solution to separate macromolecules and subcellular components. (CW)

  17. 9 CFR 3.101 - Facilities, general.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... located to prevent food contamination. Food, supplements, and medications may not be used beyond commonly... with all applicable Federal, State, and local pollution control laws. (d) Storage. Supplies of food... (harmful microbial growth), and vermin or other contamination. Refrigerators and freezers (or chilled and...

  18. Vascular Blood Collection stowage in MELFI

    NASA Image and Video Library

    2010-08-26

    ISS024-E-012546 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, prepares to insert biological samples in a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  19. Vascular Blood Collection protocol samples into MELFI

    NASA Image and Video Library

    2011-10-18

    iss029e028495 (10/18/2011) --- Japan Aerospace Exploration Agency astronaut Satoshi Furukawa,Expedition 29 flight engineer,prepares to put samples from the CSA (Canadian Space Agency) Vascular Blood Collection protocol into the MELFI-1 (Minus Eighty Laboratory Freezer for ISS 1) unit.

  20. Vascular Blood Collection stowage in MELFI

    NASA Image and Video Library

    2010-08-26

    ISS024-E-012548 (26 Aug. 2010) --- NASA astronaut Tracy Caldwell Dyson, Expedition 24 flight engineer, prepares to insert biological samples in a dewar tray in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  1. 3. Ice Plant interior, west section, looking north. Crib wall ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Ice Plant interior, west section, looking north. Crib wall of stacked 4' X 6' 's in foreground. Opening leads to refrigeration equipment area. Freezer room to the rear. - Curtis Wharf, Ice Plant, O & Second Streets, Anacortes, Skagit County, WA

  2. Listing of Food Service Equipment.

    ERIC Educational Resources Information Center

    National Sanitation Foundation, Ann Arbor, MI. Testing Lab.

    A comprehensive listing of food service equipment including--(1) companies authorized to use the National Sanitation Foundation seal of approval, and (2) equipment listed as meeting NSF standards including soda fountains, spray-type dishwashers, dishwashing equipment, cooking equipment, commerical cooking and warming equipment, freezers,…

  3. JPRS Report, East Europe.

    DTIC Science & Technology

    1988-03-02

    months, an additional refrigeration storage plant with a freezer tun- nel. Using credit from the Swiss firm TRADAX, a horticultural combine in...it is not its therapeutic value, but rather that many mistakes have become clearer. The first mistake is that its proponents believed that the

  4. Viking GCMS Data Restoral and Perceiving Temperature on Other Worlds: Astrobiology Projects at NASA Ames

    NASA Technical Reports Server (NTRS)

    Guzman, Melissa

    2015-01-01

    The primary task for the summer was to procure the GCMS data from the National Space Science Data Coordinated Archive (NSSDCA) and to assess the current state of the data set for possible reanalysis opportunities. After procurement of the Viking GCMS data set and analysis of its current state, the internship focus shifted to preparing a plan for restoral and archiving of the GCMS data set. A proposal was prepared and submitted to NASA Headquarters to restore and make available the 8000 mass chromatographs that are the basic data generated by the Viking GCMS instrument. The relevance of this restoral and the methodology we propose for restoral is presented. The secondary task for the summer is to develop a thermal model for the perceived temperature of a human standing on Mars, Titan, or Europa. Traditionally, an equation called "Fanger's comfort equation" is used to measure the perceived temperature by a human in a given reference environment. However, there are limitations to this model when applied to other planets. Therefore, the approach for this project has been to derive energy balance equations from first principles and then develop a methodology for correlating "comfort" to energy balance. Using the -20 C walk-in freezer in the Space Sciences building at NASA Ames, energy loss of a human subject is measured. Energy loss for a human being on Mars, Titan and Europa are calculated from first principles. These calculations are compared to the freezer measurements, e.g. for 1 minute on Titan, a human loses as much energy as x minutes in a -20 C freezer. This gives a numerical comparison between the environments. These energy calculations are used to consider the physiological comfort of a human based on the calculated energy losses.

  5. 2nd NUTRITION w/Repository collection period

    NASA Image and Video Library

    2011-10-04

    ISS029-E-015096 (4 Oct. 2011) --- In the International Space Station?s Kibo laboratory, NASA astronaut Mike Fossum, Expedition 29 commander, prepares to insert samples into a Minus Eighty Laboratory Freezer for ISS (MELFI-1) dewar tray for the second NUTRITION w/Repository collection period.

  6. 2nd NUTRITION w/Repository collection period

    NASA Image and Video Library

    2011-10-04

    ISS029-E-015110 (4 Oct. 2011) --- In the International Space Station?s Kibo laboratory, NASA astronaut Mike Fossum, Expedition 29 commander, inserts a dewar tray of samples into the Minus Eighty Laboratory Freezer for ISS (MELFI-1) for the second NUTRITION w/Repository collection period.

  7. iss050e057428

    NASA Image and Video Library

    2017-03-15

    iss050e057428 (03/15/2017) --- NASA astronaut Shane Kimbrough removes a storage locker in the Minus Eighty-degree Laboratory Freezer for ISS (MELFI) to store samples from an experiment. MELFI is a cold storage unit that maintains experiment samples at ultra-cold temperatures throughout a mission.

  8. Army Sustainment. Volume 42, Issue 2, March-April 2010

    DTIC Science & Technology

    2010-04-01

    and provides additional keys to the recruiter for mission attainment. This element provides insight on how to— ❏ Establish instant rapport...catering for more than 500 personnel, electronic ovens, freezers, and machines to make noodles and bean curd were introduced.10 Rear-echelon units

  9. Tested Demonstrations.

    ERIC Educational Resources Information Center

    Gilbert, George L., Ed.

    1987-01-01

    Describes two laboratory demonstrations in chemistry. One uses dry ice, freon, and freezer bags to demonstrate volume changes, vapor-liquid equilibrium, a simulation of a rain forest, and vaporization. The other uses the clock reaction technique to illustrate fast reactions and kinetic problems in releasing carbon dioxide during respiration. (TW)

  10. 40 CFR 60.451 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...-coated metal range, oven, microwave oven, refrigerator, freezer, washer, dryer, dishwasher, water heater... submerged in a tank filled with coating material suspended in water and an electrical potential is used to... is fused into a continuous coating film through the use of heat. Spray booth means the structure...

  11. 40 CFR 60.451 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...-coated metal range, oven, microwave oven, refrigerator, freezer, washer, dryer, dishwasher, water heater... submerged in a tank filled with coating material suspended in water and an electrical potential is used to... is fused into a continuous coating film through the use of heat. Spray booth means the structure...

  12. 40 CFR 60.451 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...-coated metal range, oven, microwave oven, refrigerator, freezer, washer, dryer, dishwasher, water heater... submerged in a tank filled with coating material suspended in water and an electrical potential is used to... is fused into a continuous coating film through the use of heat. Spray booth means the structure...

  13. 40 CFR 60.451 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-coated metal range, oven, microwave oven, refrigerator, freezer, washer, dryer, dishwasher, water heater... submerged in a tank filled with coating material suspended in water and an electrical potential is used to... is fused into a continuous coating film through the use of heat. Spray booth means the structure...

  14. 40 CFR 60.451 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...-coated metal range, oven, microwave oven, refrigerator, freezer, washer, dryer, dishwasher, water heater... submerged in a tank filled with coating material suspended in water and an electrical potential is used to... is fused into a continuous coating film through the use of heat. Spray booth means the structure...

  15. 10 CFR 431.373 - Enforcement.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... may conduct a review of the test records. The Secretary may then conduct enforcement testing of that...) For automatic commercial ice makers, as well as commercial refrigerators, freezers, and refrigerator... numbers to select the units to be tested. (ii) For automatic commercial ice makers, as well as commercial...

  16. Skylab

    NASA Image and Video Library

    1972-01-01

    This photograph, with callouts, depicts the experiment area of the forward compartment at the upper level of the Orbital Workshop. The upper level consisted of a large work area and housed water storage tanks, a food freezer, storage vaults for film, scientific airlocks, mobility and stability experiment equipment, and other experimental equipment.

  17. 10 CFR 431.64 - Uniform test method for the measurement of energy consumption of commercial refrigerators...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Refrigerated Volume and Total Shelf Area of Household Refrigerators and Household Wine Chillers,” and sections... Chillers and Freezers.” [70 FR 60414, Oct. 18, 2005, as amended at 77 FR 10318, Feb. 21, 2012] Energy...

  18. 10 CFR 431.64 - Uniform test method for the measurement of energy consumption of commercial refrigerators...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Refrigerated Volume and Total Shelf Area of Household Refrigerators and Household Wine Chillers,” and sections... Chillers and Freezers.” [70 FR 60414, Oct. 18, 2005, as amended at 77 FR 10318, Feb. 21, 2012] Energy...

  19. Cold Stowage Flight Systems

    NASA Technical Reports Server (NTRS)

    Campana, Sharon E.; Melendez, David T.

    2011-01-01

    The International Space Station (ISS) provides a test bed for researchers to perform science experiments in a variety of fields, including human research, life sciences, and space medicine. Many of the experiments being conducted today require science samples to be stored and transported in a temperature controlled environment. NASA provides several systems which aid researchers in preserving their science. On orbit systems provided by NASA include the Minus Eighty Laboratory freezer for ISS (MELFI), Microgravity Experiment Research Locker Incubator (MERLIN), and Glacier. These freezers use different technologies to provide rapid cooling and cold stowage at different temperature levels on board ISS. Systems available to researchers during transportation to and from ISS are MERLIN, Glacier, and Coldbag. Coldbag is a passive cold stowage system that uses phase change materials to maintain temperature. Details of these current technologies are provided along with operational experience gained to date. This paper discusses the capability of the current cold stowage hardware and how it may continue to support NASA s mission on ISS and in future exploration missions.

  20. Development of a refrigeration system for lunar surface and spacecraft applications

    NASA Technical Reports Server (NTRS)

    Copeland, R. J.

    1976-01-01

    An evaluation of refrigeration devices suitable for potential lunar surface and spacecraft applications was performed. The following conclusions were reached: (1) the vapor compression system is the best overall refrigeration system for lunar surface and spacecraft applications and the single phase radiator system is generally preferred for earth orbit applications, (2) the vapor compression cycle may have some application for simultaneous heating and cooling, (3) a Stirling cycle refrigerator was selected for the manned cabin of the space shuttle, and (4) significant increases in payload heat rejection can be obtained by a kit vapor compression refrigerator added to the shuttle R-21 loop. The following recommendations were made: (1) a Stirling cycle refrigerator may be used for food freezer and biomedical sample storage, (2) the best system for a food freezer/experiments compartment for an earth orbit space station has not been determined, (3) a deployed radiator system can be designed for large heat loads in earth orbit.

  1. Could cryopreserved human semen samples be stored at -80°C?

    PubMed

    Vaz, Carlos R; Lamim, Tamara; Salvador, Rafael A; Batschauer, Anna P B; Amaral, Vera Lucia L; Til, David

    2018-06-01

    To evaluate storage time effects in cryopreserved human semen samples, kept in the freezer at a controlled temperature of -80°C, on sperm viability after thawing. We used 20 semen samples. Each sample was cryopreserved in 10 fingers, which were divided into five groups: one group was kept in cryogenic canisters throughout the experiment(control), and four groups were kept in a VIP Ultra Low MDF-U76V- PE freezer, with the temperature set at -80°C, for 24, 48, 72 and 96 hours, respectively. After the exposure time, the samples were stored in cryogenic canisters after being thawed. The analyzed parameters were: motility, vitality and mitochondrial activity. After thawing, we noticed decreased sperm motility, vitality and mitochondrial activity, when comparing the tested groups with the control group, as well as a progressive reduction in the analyzed parameters between the times evaluated. Cryopreservation of semen samples at -80°C is potentially harmful to sperm viability, causing damage when submitted to longer exposure times.

  2. Analysis of household refrigerators for different testing standards

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bansal, P.K.; McGill, I.

    This study highlights the salient differences among various testing standards for household refrigerator-freezers and proposes a methodology for predicting the performance of a single evaporator-based vapor-compression refrigeration system (either refrigerator or freezer) from one test standard (where the test data are available-the reference case) to another (the alternative case). The standards studied during this investigation include the Australian-New Zealand Standard (ANZS), the International Standard (ISO), the American National Standard (ANSI), the Japanese Industrial Standard (JIS), and the Chinese National Standard (CNS). A simple analysis in conjunction with the BICYCLE model (Bansal and Rice 1993) is used to calculate the energymore » consumption of two refrigerator cabinets from the reference case to the alternative cases. The proposed analysis includes the effect of door openings (as required by the JIS) as well as defrost heaters. The analytical results are found to agree reasonably well with the experimental observations for translating energy consumption information from one standard to another.« less

  3. Design and development of a freezer and chiller delivery box

    NASA Astrophysics Data System (ADS)

    Zakaria, Mohd Zakimi; Hung, Goh Chung; Dawi, Mohd Syedi Imran Mohd; Hussin, Radhwan; Khalil, Ahmad Nabil Mohd; Naim, Muhammad Khairy Md; Hilmi, Ahmad Humaizi

    2017-09-01

    This paper presents an action research of designed and fabricated using well insulating materials in order to ensure the coolness inside the freezer and chiller delivery box is as good minimize temperature raised. The main purpose of this study is to develop freeze and chiller delivery box that will be able to keep fresh meat during travelling long delivery. A range of freeze and chill solutions exists for that must be kept within a specific temperature range throughout the supply-and-distribution chain. This will help to minimize the activeness of bacteria to spoil the meats, at the same time it can linger the duration for meats to spoil. All affecting parameter such as temperature inside the delivery box, heat transfer rate, and natural convection flow pattern has been studied to design and development of the delivery box. Finally, temperature distribution analysis has been conducted which showed that in operating condition inside temperature are suitable to keep the fresh meats condition.

  4. Thirsk in JPM

    NASA Image and Video Library

    2009-06-06

    ISS020-E-007147 (6 June 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  5. Coleman prepares to insert samples into the MELFI

    NASA Image and Video Library

    2010-12-27

    ISS026-E-023814 (27 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, works with the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  6. Thirsk in JPM

    NASA Image and Video Library

    2009-06-06

    ISS020-E-007140 (6 June 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  7. MELFI Urine Sample First Insertion

    NASA Image and Video Library

    2009-04-11

    ISS019-E-005715 (11 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  8. Garan works with MELFI in Kibo

    NASA Image and Video Library

    2011-04-13

    iss027e014283 (4/13/2011) --- Expedition 27 flight engineer Ron Garan prepares to stow a blood draw for the CSA (Canadian Space Agency) Vascular Blood Collection protocol in the Minus Eighty Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  9. 50 CFR 660.216 - Fixed gear fishery-observer requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES OFF WEST COAST STATES... all USCG and other applicable rules, regulations, or statutes pertaining to safe operation of the..., processing areas, freezer spaces, weight scales, cargo holds, and any other space that may be used to hold...

  10. 50 CFR 660.216 - Fixed gear fishery-observer requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES OFF WEST COAST STATES... applicable rules, regulations, or statutes pertaining to safe operation of the vessel, and provisions at..., freezer spaces, weight scales, cargo holds, and any other space that may be used to hold, process, weigh...

  11. 50 CFR 660.216 - Fixed gear fishery-observer requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES OFF WEST COAST STATES... all USCG and other applicable rules, regulations, or statutes pertaining to safe operation of the..., processing areas, freezer spaces, weight scales, cargo holds, and any other space that may be used to hold...

  12. 77 FR 72763 - Energy Conservation Program: Certification of Commercial and Industrial HVAC, Refrigeration and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-06

    ... Commercial and Industrial HVAC, Refrigeration and Water Heating Equipment AGENCY: Office of Energy Efficiency... extension to the compliance date for the certification provisions of commercial refrigeration equipment... refrigeration equipment; commercial HVAC equipment; commercial WH equipment; and walk-in coolers and freezers...

  13. 16 CFR Appendix B1 to Part 305 - Upright Freezers With Manual Defrost

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  14. 16 CFR Appendix B2 to Part 305 - Upright Freezers With Automatic Defrost

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  15. 16 CFR Appendix B2 to Part 305 - Upright Freezers With Automatic Defrost

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  16. 16 CFR Appendix B1 to Part 305 - Upright Freezers With Manual Defrost

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  17. 16 CFR Appendix B2 to Part 305 - Upright Freezers With Automatic Defrost

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  18. 16 CFR Appendix B1 to Part 305 - Upright Freezers With Manual Defrost

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  19. 16 CFR Appendix B1 to Part 305 - Upright Freezers With Manual Defrost

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  20. 16 CFR Appendix B2 to Part 305 - Upright Freezers With Automatic Defrost

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ... (*) (*) (*) No data submitted for units meeting the Department of Energy's Energy Conservation Standards...

  1. Skylab

    NASA Image and Video Library

    1972-01-01

    This image, with callouts, depicts the storage area of the forward compartment at the upper level of the Orbital Workshop (OWS). The upper level consisted of a large work area and housed water storage tanks, a food freezer, storage vaults for film, scientific airlocks, mobility and stability experiment equipment, and other experimental equipment.

  2. What Happens to Donated Blood?

    MedlinePlus

    ... freezers for up to one year. Step Five Distribution Blood is available to be shipped to hospitals 24 hours a day, 7 days a week. Hospitals typically keep some blood units on their shelves, but may call for more at any time, such as in case of large scale emergencies. ...

  3. U.S. Residential Miscellaneous Refrigeration Products: Results from Amazon Mechanical Turk Surveys

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Greenblatt, Jeffery B.; Young, Scott J.; Yang, Hung-Chia

    Amazon Mechanical Turk was used, for the first time, to collect statistically representative survey data from U.S. households on the presence, number, type and usage of refrigerators, freezers, and various “miscellaneous” refrigeration products (wine/beverage coolers, residential icemakers and non-vapor compression refrigerators and freezers), along with household and demographic information. Such products have been poorly studied to date, with almost no information available about shipments, stocks, capacities, energy use, etc. A total of 9,981 clean survey responses were obtained from five distinct surveys deployed in 2012. General refrigeration product survey responses were weighted to demographics in the U.S. Energy Information Administration’smore » Residential Energy Consumption Survey 2009 dataset. Miscellaneous refrigeration product survey responses were weighted according to demographics of product ownership found in the general refrigeration product surveys. Model number matching for a portion of miscellaneous refrigeration product responses allowed validation of refrigeration product characteristics, which enabled more accurate estimates of the penetrations of these products in U.S. households. We estimated that there were 12.3±1.0 million wine/beverage coolers, 5.5(–3.5,+3.2) million residential icemakers and 4.4(–2.7,+2.3) million non-vapor compression refrigerators in U.S. households in 2012. (All numerical results are expressed with ranges indicating the 95% confidence interval.) No evidence was found for the existence of non-vapor compression freezers. Moreover, we found that 15% of wine/beverage coolers used vapor compression cooling technology, while 85% used thermoelectric cooling technology, with the vast majority of thermoelectric units having capacities of less than 30 wine bottles (approximately 3.5 cubic feet). No evidence was found for the existence of wine/beverage coolers with absorption cooling technology. Additionally, we estimated that there were 3.6±1.0 million hybrid refrigerator-wine/beverage coolers and 0.9±0.5 million hybrid freezer-wine/beverage coolers in U.S. households. We also obtained estimates of miscellaneous refrigeration product capacities, lifetimes, purchase and installation costs, repair frequencies and costs, and maintenance costs. For wine/beverage coolers, we also obtained information on the penetration of built-in units, AC/DC operating capability, the use of internal lights, and distributions of door opening frequencies. This information is essential to develop detailed estimates of national energy usage and life-cycle costs, and would be helpful in obtaining information on other plug-load appliances. Additional information not highlighted in the main report was presented in Appendices.« less

  4. U.S. Residential Miscellaneous Refrigeration Products: Results from Amazon Mechanical Turk Surveys

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Greenblatt, Jeffery B.; Young, Scott J.; Yang, Hung-Chia

    Amazon Mechanical Turk was used, for the first time, to collect statistically representative survey data from U.S. households on the presence, number, type and usage of refrigerators, freezers, and various “miscellaneous” refrigeration products (wine/beverage coolers, residential icemakers and non-vapor compression refrigerators and freezers), along with household and demographic information. Such products have been poorly studied to date, with almost no information available about shipments, stocks, capacities, energy use, etc. A total of 9,820 clean survey responses were obtained from four distinct surveys deployed in 2012. General refrigeration product survey responses were weighted to demographics in the U.S. Energy Information Administration’smore » Residential Energy Consumption Survey 2009 dataset. Miscellaneous refrigeration product survey responses were weighted according to demographics of product ownership found in the general refrigeration product surveys. Model number matching for a portion of miscellaneous refrigeration product responses allowed validation of refrigeration product characteristics, which enabled more accurate estimates of the penetrations of these products in U.S. households. We estimated that there were 12.3±1.0 million wine/beverage coolers, 5.5(–3.5,+3.2) million residential icemakers and 2.9(–2.5,+4.5) million non-vapor compression refrigerators in U.S. households in 2012. (All numerical results are expressed with ranges indicating the 95% confidence interval.) No evidence was found for the existence of non-vapor compression freezers. Moreover, we found that 15% of wine/beverage coolers used vapor compression cooling technology, while 85% used thermoelectric cooling technology, with the vast majority of thermoelectric units having capacities of less than 30 wine bottles (approximately 3.5 cubic feet). No evidence was found for the existence of wine/beverage coolers with absorption cooling technology. Additionally, we estimated that there were 3.6±1.0 million hybrid refrigerator-wine/beverage coolers and 0.9±0.5 million hybrid freezer-wine/beverage coolers in U.S. households. We also obtained estimates of miscellaneous refrigeration product capacities, lifetimes, purchase and installation costs, repair frequencies and costs, and maintenance costs. For wine/beverage coolers, we also obtained information on the penetration of built-in units, AC/DC operating capability, the use of internal lights, and distributions of door opening frequencies. This information is essential to develop detailed estimates of national energy usage and life-cycle costs, and would be helpful in obtaining information on other plug-load appliances. Additional information not highlighted in the main report was presented in Appendices.« less

  5. 75 FR 4539 - Energy Conservation Program for Consumer Products: Publication of the Petition for Waiver and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-28

    ... procedure for determining the energy consumption of electric refrigerators and refrigerator-freezers. The... condensation. The existing test procedure does not take humidity or adaptive control technology into account. Therefore, Electrolux has suggested an alternate test procedure that takes adaptive control technology into...

  6. Corn and sorghum studies by the usda "Ag Lab" in 2012

    USDA-ARS?s Scientific Manuscript database

    Studies again included several popcorn fields in 2012. The research has been redirected to investigate environmental influences on gene expression directly and indirectly involved in resistance to mycotoxin production, so milk stage undamaged and damaged ears have been saved in the freezer for the g...

  7. 9 CFR 121.17 - Records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS POSSESSION, USE, AND TRANSFER OF SELECT... ensure viability for future use, such as in a freezer or lyophilized materials), including: (i) The name... by whom and when returned to storage and by whom; (v) The select agent used and purpose of use; (vi...

  8. 75 FR 41103 - Energy Conservation Program: Re-Opening of the Public Comment Period for Walk-In Coolers and Walk...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... DEPARTMENT OF ENERGY 10 CFR Part 431 [Docket No. EERE-2008-BT-STD-0015] RIN 1904-AB86 Energy... preliminary analysis for walk-in coolers and walk-in freezers, and provide docket number EERE-2008-BT-STD-0015...

  9. Nutrition and Repository: Insertion of Urine Sample into MELFI

    NASA Image and Video Library

    2009-04-18

    ISS019-E-010170 (18 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  10. Coleman prepares to insert samples into the MELFI

    NASA Image and Video Library

    2010-12-27

    ISS026-E-023768 (27 Dec. 2010) --- NASA astronaut Catherine (Cady) Coleman, Expedition 26 flight engineer, prepares to insert samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  11. Nutrition and Repository: Insertion of Urine Sample into MELFI

    NASA Image and Video Library

    2009-04-18

    ISS019-E-010165 (18 April 2009) --- Astronaut Michael Barratt, Expedition 19/20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  12. 7 CFR 58.638 - Freezing the mix.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Freezing the mix. 58.638 Section 58.638 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Procedures § 58.638 Freezing the mix. After the mix enters the freezer, it shall be frozen as rapidly as...

  13. 7 CFR 58.638 - Freezing the mix.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Freezing the mix. 58.638 Section 58.638 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Procedures § 58.638 Freezing the mix. After the mix enters the freezer, it shall be frozen as rapidly as...

  14. 7 CFR 58.638 - Freezing the mix.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Freezing the mix. 58.638 Section 58.638 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... Procedures § 58.638 Freezing the mix. After the mix enters the freezer, it shall be frozen as rapidly as...

  15. Making an Ice Core.

    ERIC Educational Resources Information Center

    Kopaska-Merkel, David C.

    1995-01-01

    Explains an activity in which students construct a simulated ice core. Materials required include only a freezer, food coloring, a bottle, and water. This hands-on exercise demonstrates how a glacier is formed, how ice cores are studied, and the nature of precision and accuracy in measurement. Suitable for grades three through eight. (Author/PVD)

  16. Hadfield prepares to insert biological samples in the MELFI-1

    NASA Image and Video Library

    2013-01-07

    View of Canadian Space Agency (CSA) Chris Hadfield,Expedition 34 Flight Engineer (FE),preparing to insert biological samples in the Minus Eighty Laboratory Freezer for International Space Station (ISS) - (MELFI-1),in the Japanese Experiment Module (JEM) Pressurized Module (JPM). Photo was taken during Expedition 34.

  17. 16 CFR Appendix A2 to Part 305 - Refrigerators and Refrigerators-Freezers With Manual Defrost

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION... for units meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72...

  18. 16 CFR Appendix A2 to Part 305 - Refrigerators and Refrigerators-Freezers With Manual Defrost

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION... for units meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72...

  19. 16 CFR Appendix A2 to Part 305 - Refrigerators and Refrigerators-Freezers With Manual Defrost

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION... for units meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72...

  20. 16 CFR Appendix A2 to Part 305 - Refrigerators and Refrigerators-Freezers With Manual Defrost

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION... for units meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72...

  1. 75 FR 17080 - Energy Conservation Standards for Walk-in Coolers and Walk-in Freezers: Public Meeting and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-05

    ... Preliminary Technical Support Document AGENCY: Office of Energy Efficiency and Renewable Energy, Department of Energy. ACTION: Notice of public meeting and availability of preliminary technical support document... consider for this equipment. In addition, DOE encourages written comments on these subjects. To inform...

  2. 78 FR 63166 - Certain Kitchen Appliance Shelving and Racks From the People's Republic of China: Countervailing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-23

    ... Shelving and Racks From the People's Republic of China: Countervailing Duty Administrative Review; 2011... order on certain kitchen appliance shelving and racks (kitchen racks) from the People's Republic of.... Scope of the Order This order covers shelving and racks for refrigerators, freezers, combined...

  3. 21 CFR 640.54 - Processing.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Cryoprecipitate § 640.54 Processing. (a) Processing the plasma. (1) The plasma shall be separated from the red blood cells by centrifugation to obtain essentially cell-free plasma. (2) The plasma shall be placed in a freezer within 8 hours after blood collection or...

  4. 21 CFR 640.54 - Processing.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Cryoprecipitate § 640.54 Processing. (a) Processing the plasma. (1) The plasma shall be separated from the red blood cells by centrifugation to obtain essentially cell-free plasma. (2) The plasma shall be placed in a freezer within 8 hours after blood collection or...

  5. 21 CFR 640.54 - Processing.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Cryoprecipitate § 640.54 Processing. (a) Processing the plasma. (1) The plasma shall be separated from the red blood cells by centrifugation to obtain essentially cell-free plasma. (2) The plasma shall be placed in a freezer within 8 hours after blood collection or...

  6. 21 CFR 640.54 - Processing.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Cryoprecipitate § 640.54 Processing. (a) Processing the plasma. (1) The plasma shall be separated from the red blood cells by centrifugation to obtain essentially cell-free plasma. (2) The plasma shall be placed in a freezer within 8 hours after blood collection or...

  7. 21 CFR 640.54 - Processing.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Cryoprecipitate § 640.54 Processing. (a) Processing the plasma. (1) The plasma shall be separated from the red blood cells by centrifugation to obtain essentially cell-free plasma. (2) The plasma shall be placed in a freezer within 8 hours after blood collection or...

  8. 76 FR 52313 - Bottom Mount Combination Refrigerator-Freezers From the Republic of Korea and Mexico...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-22

    ... Administration, Department of Commerce. FOR FURTHER INFORMATION CONTACT: Henry Almond (Republic of Korea) (202..., 2011, Whirlpool Corporation (hereafter, the petitioner) made timely requests, pursuant to 19 CFR 351... request for an extension of the period within which the preliminary determination must be made under...

  9. 77 FR 5784 - Decision and Order Granting a Waiver to Sub-Zero From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-06

    ... electric refrigerator and refrigerator-freezer test procedures for determining the energy consumption of... compressors using an alternate test procedure that takes this technology into account when measuring energy.... Bryan Berringer, U.S. Department of Energy, Building Technologies Program, Mailstop EE-2J, 1000...

  10. 75 FR 51262 - Energy Conservation Program for Consumer Products: Decision and Order Granting a Waiver to GE...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-19

    ... General Electric Company (GE) a waiver from the DOE electric refrigerator and refrigerator-freezer test... procedure that takes this technology into account when measuring energy consumption. DATES: This Decision.... Department of Energy, Building Technologies Program, Mailstop EE-2J, 1000 Independence Avenue, SW...

  11. 78 FR 38699 - Decision and Order Granting a Waiver to GE Appliances From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-27

    ... electric refrigerator and refrigerator-freezer test procedures for determining the energy consumption of... using an alternate test procedure that takes this technology into account when measuring energy.... Bryan Berringer, U.S. Department of Energy, Building Technologies Program, Mail Stop EE-2J, Forrestal...

  12. 75 FR 51264 - Energy Conservation Program for Consumer Products: Decision and Order Granting a Waiver to LG...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-19

    ... Electronics, Inc. (LG) a waiver from the DOE electric refrigerator and refrigerator- freezer test procedure... takes this technology into account when measuring energy consumption. DATES: This Decision and Order is... Energy, Building Technologies Program, Mailstop EE-2J, 1000 Independence Avenue, SW., Washington, DC...

  13. 78 FR 38696 - Decision and Order Granting a Waiver to GE Appliances From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-27

    ... electric refrigerator and refrigerator-freezer test procedures for determining the energy consumption of... test procedure that takes this technology into account when measuring energy consumption. DATES: This.... Department of Energy, Building Technologies Program, Mail Stop EE-2J, Forrestal Building, 1000 Independence...

  14. 77 FR 17410 - Bottom Mount Combination Refrigerator-Freezers From the Republic of Korea: Final Affirmative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ..., 2011, the Department issued its Post-Preliminary Analysis of Cross-ownership and its Post- Preliminary Analysis of New Subsidy Allegations. On that date, the Department also issued its Post-Preliminary Analysis... Post-Preliminary Analysis: GOK Preferential Lending Under the Daewoo Workout, and the GOK, LGE, SEC...

  15. Thirsk inserts samples into MELFI in the JPM

    NASA Image and Video Library

    2009-06-07

    ISS020-E-007156 (7 June 2009) --- Canadian Space Agency astronaut Robert Thirsk, Expedition 20 flight engineer, performs an insertion of urine samples into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) as part of the Nutritional Status Assessment (NUTRITION) study in the Japanese Kibo laboratory of the International Space Station.

  16. NUTRITION w/Repository into MELFI

    NASA Image and Video Library

    2009-07-30

    ISS020-E-026348 (30 July 2009) --- Astronaut Tim Kopra, Expedition 20 flight engineer, smiles for the camera as he completes the task of placing samples taken for the Nutritional Status Assessment (NUTRITION) w/Repository study into the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) in the Kibo laboratory of the International Space Station.

  17. 76 FR 70957 - Foreign-Trade Zone 277-Western Maricopa County, AZ; Application for Manufacturing Authority, Sub...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-16

    ..., and wine storage units for export and the domestic market. Components and materials sourced from... entry procedures that applies to refrigerators, freezers, and wine storage units (duty rate--free) for... realize logistical benefits through the use of weekly customs entry procedures. Customs duties also could...

  18. Disaster Planning Guidelines for Fire Chiefs.

    DTIC Science & Technology

    1980-07-01

    book Money Receipts e I pad white squared paper * I roll masking tape (8 X 13) (-" × 60yds) * 3 spring clips * 3 felt tip markers (red, e I pkg. freezer...Avenue COMMIT EE HaRm Washington. D.C. 20012 C.Holy Redeemer Rectory (202) 723-0800 C. Neil Molenaar 9705 Summit Avenue Director, Domestic Programs

  19. 76 FR 19125 - Bottom Mount Combination Refrigerator-Freezers From Korea and Mexico

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-06

    ... by the Government of Korea. Unless the Department of Commerce extends the time for initiation... investigations in 45 days, or in this case by May 16, 2011. The Commission's views are due at Commerce within five business days thereafter, or by May 23, 2011. For further information concerning the conduct of...

  20. 77 FR 49443 - Decision and Order Granting a Waiver to Sanyo From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-16

    ... DEPARTMENT OF ENERGY Office of Energy Efficiency and Renewable Energy [Case No. RF-022] Decision... Refrigerator-Freezer Test Procedures AGENCY: Office of Energy Efficiency and Renewable Energy, Department of... characteristics. The Assistant Secretary for Energy Efficiency and Renewable Energy (the Assistant Secretary) may...

  1. 77 FR 75428 - Decision and Order Granting a Waiver to Samsung From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... DEPARTMENT OF ENERGY Office of Energy Efficiency and Renewable Energy [Case No. RF-021] Decision... Refrigerator-Freezer Test Procedures AGENCY: Office of Energy Efficiency and Renewable Energy, Department of... Assistant Secretary for Energy Efficiency, Energy Efficiency and Renewable Energy. Decision and Order In the...

  2. 10 CFR 429.53 - Walk-in coolers and walk-in freezers.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... insulation, and a declaration that the manufacturer has incorporated the applicable design requirements. In... windows (e.g., double-pane with heat reflective treatment, triple-pane glass with gas fill), and the power...-value of the insulation (except for glazed portions of the doors or structural members) (iii) For WICF...

  3. 76 FR 21579 - Energy Conservation Program: Test Procedures for Walk-In Coolers and Walk-In Freezers

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-15

    ..., ``Thermal insulation products for buildings--Factory made products of extruded polystyrene foam (XPS)-- Specification,'' approved February 2009. (4) DIN EN 13165:2009-02, ``Thermal insulation products for buildings... 2. Heat Transfer Through Panels 3. Energy Use of Doors 4. Heat Transfer via Air Infiltration 5...

  4. Tips for Energy Savers.

    ERIC Educational Resources Information Center

    Department of Energy, Washington, DC.

    According to 1986 U.S. Department of Energy data, 48% of our residential energy is used to heat and cool our homes, 16% goes for heating water, 12% is used to refrigerators and freezers, and the remaining 24% goes into lighting, cooking, and running appliances. This booklet contains tips for saving energy, including sections on: (1) draft-proof…

  5. 10 CFR 429.53 - Walk-in coolers and walk-in freezers.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... insulation, and a declaration that the manufacturer has incorporated the applicable design requirements. In... windows (e.g., double-pane with heat reflective treatment, triple-pane glass with gas fill), and the power...-value of the insulation (except for glazed portions of the doors or structural members) (iii) For WICF...

  6. 76 FR 23298 - Bottom Mount Combination Refrigerator-Freezers From the Republic of Korea: Initiation of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-26

    .... Changwon City Provision of Waste Heat Electricity For further information explaining why the Department is..., (e) wiring, and (f) insulation; (2) any assembled external doors designed for use in bottom mount... plastic liner, and (c) insulation; and (3) any assembled external drawers designed for use in bottom mount...

  7. 10 CFR 429.53 - Walk-in coolers and walk-in freezers.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... insulation, and a declaration that the manufacturer has incorporated the applicable design requirements. In... windows (e.g., double-pane with heat reflective treatment, triple-pane glass with gas fill), and the power...-value of the insulation (except for glazed portions of the doors or structural members) (iii) For WICF...

  8. 26 CFR 1.1241-1 - Cancellation of lease or distributor's agreement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... marketing or marketing and servicing of goods. It does not apply to agreements for selling intangible... his State. The marketing is accomplished by using the warehouse and trucks acquired before he entered... distributes to frozen-food freezer and locker customers. The terms of his distributorship do not make it...

  9. 76 FR 23281 - Bottom Mount Combination Refrigerator-Freezers From the Republic of Korea and Mexico: Initiation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-26

    ... feel are relevant to the development of an accurate listing of physical characteristics. Specifically... two interior storage compartments accessible through one or more separate external doors or drawers or... external door or drawer is either a refrigerator compartment or convertible compartment, but is not a...

  10. 76 FR 78832 - Protection of Stratospheric Ozone: Listing of Substitutes for Ozone-Depleting Substances...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-20

    ... outside the home. Household freezers offer storage space only at freezing temperatures. Products with both... systems, including cold storage cases, designed to chill food or keep it at a cold temperature for... Administration NOAEL--no observable adverse effect level NPRM--notice of proposed rulemaking NTTAA--National...

  11. 75 FR 55067 - Energy Conservation Program: Test Procedures for Walk-In Coolers and Walk-In Freezers

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-09

    ..., 1000 Independence Avenue, SW., Washington, DC 20585-0121. Please submit one signed original paper copy... Program, 950 L'Enfant Plaza, 6th Floor, Washington, DC 20024. Please submit one signed original paper copy...: [email protected] . SUPPLEMENTARY INFORMATION: I. Authority and Background II. Summary of the...

  12. MELFI / GLACIER Transfers

    NASA Image and Video Library

    2013-03-12

    ISS034-E-067263 (12 March 2013) --- Canadian astronaut Chris Hadfield, right, assists fellow Expedition 34 flight engineer and NASA astronaut Tom Marshburn during Minus Eighty-Degree Laboratory Freezer for International Space Station (MELFI)operations. The two are doing transfers of samples connected to the General Laboratory Active Cryogenic ISS Experiment Refrigerator or GLACIER in the U.S. lab Destiny.

  13. 16 CFR Appendix A3 to Part 305 - Refrigerator-Freezers With Partial Automatic Defrost

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE... meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72 FR 49975, Aug...

  14. 16 CFR Appendix A3 to Part 305 - Refrigerator-Freezers With Partial Automatic Defrost

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE... meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72 FR 49975, Aug...

  15. 16 CFR Appendix A3 to Part 305 - Refrigerator-Freezers With Partial Automatic Defrost

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE... meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72 FR 49975, Aug...

  16. 16 CFR Appendix A3 to Part 305 - Refrigerator-Freezers With Partial Automatic Defrost

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... SPECIFIC ACTS OF CONGRESS RULE CONCERNING DISCLOSURES REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE... meeting the Department of Energy's Energy Conservation Standards effective July 1, 2001. [72 FR 49975, Aug...

  17. 77 FR 29593 - North Pacific Fishery Management Council; Public Meetings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-18

    ... Particular Concern (HAPC) skate egg sites. 4. BSAI Crab Rebuilding Crab Plan Team report on Set catch Specifications for 4 stocks; Final action on Pribilof Bristol Bay Red King Crab rebuilding plan. 5. Freezer... and tasking. 8. Other Business The SSC agenda will include the following issues: 1. EFH 2. BSAI Crab...

  18. 21 CFR 1250.86 - Water for making ice.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Water for making ice. 1250.86 Section 1250.86 Food... SANITATION Sanitation Facilities and Conditions on Vessels § 1250.86 Water for making ice. Only potable water shall be piped into a freezer for making ice for drinking and culinary purposes. ...

  19. 16 CFR 305.3 - Description of covered products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...-freezers. (1) Electric refrigerator means a cabinet designed for the refrigerated storage of food, designed... temperatures below 32 °F (0 °C), but does not provide a separate low temperature compartment designed for the... a cabinet which consists of two or more compartments with at least one of the compartments designed...

  20. Adam's Dream

    ERIC Educational Resources Information Center

    Piacenti, Alexandria

    2011-01-01

    Growing up as a young girl, summer camp was a typical affair. Packed lunches kept cold with frozen freezer packs, seemingly endless slip n' slides, activities, games, contests. Every morning, the author was eager to wake up and head off to hours and hours of fun with friends and counselors. However, one may think the camping experience could not…

  1. Saving Energy around the House = Tien Tan Trong Viec Tieu Thu Nang Luc Trong Nha.

    ERIC Educational Resources Information Center

    Noyes, Marilyn; Jarrett, Von

    This bilingual booklet is intended to help Vietnamese refugees learn basic energy conservation skills. Included in the booklet are Vietnamese and English translations of basic energy conservation practices related to the following areas: heating, cooling, cooking, using refrigerators and freezers, lighting, water heating, doing laundry, pursuing…

  2. From forest to freezer: a comprehensive seed collection of the Kentucky coffeetree, Gymnocladus dioicus (L.) K

    Treesearch

    A.P. Schmitz; J.D. Carstens

    2017-01-01

    Kentucky coffeetree, Gymnocladus dioicus, is a picturesque shade tree adaptable to urban conditions and drought, with no serious insect or disease problems. These traits make G. dioicus a promising candidate among diverse tree genera to replace ash (Fraxinus) trees affected by the emerald ash borer (Agrilus...

  3. 78 FR 53374 - Energy Conservation Program for Consumer Products: Test Procedures for Refrigerators...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-29

    ... Freezers AGENCY: Office of Energy Efficiency and Renewable Energy, Department of Energy. ACTION: Extension... INFORMATION CONTACT: Mr. Lucas Adin, U.S. Department of Energy, Office of Energy Efficiency and Renewable... Energy Efficiency, Energy Efficiency and Renewable Energy. [FR Doc. 2013-21115 Filed 8-28-13; 8:45 am...

  4. Lithography of Polymer Nanostructures on Glass for Teaching Polymer Chemistry and Physics

    ERIC Educational Resources Information Center

    Sahar-Halbany, Adi; Vance, Jennifer M.; Drain, Charles Michael

    2011-01-01

    As nanolithography becomes increasingly important in technology and daily life, a variety of inexpensive and creative methods toward communicating the concepts underpinning these processes in the classroom are necessary. An experiment is described that uses simple CD-Rs, C-clamps, an oven, and a freezer to provide concrete examples and insights…

  5. 7 CFR 3560.103 - Maintaining housing projects.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... metal, free of rust. The housing project must have windows which are easily operated, free of bent... housing which is sound and the finish is free of chips, damage, or signs of rust. The range hood fan and... cooler and freezer are operating properly, the shelves and door containers are secure and free of rust...

  6. 7 CFR 3560.103 - Maintaining housing projects.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... metal, free of rust. The housing project must have windows which are easily operated, free of bent... housing which is sound and the finish is free of chips, damage, or signs of rust. The range hood fan and... cooler and freezer are operating properly, the shelves and door containers are secure and free of rust...

  7. 7 CFR 3560.103 - Maintaining housing proj-ects.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... metal, free of rust. The housing project must have windows which are easily operated, free of bent... housing which is sound and the finish is free of chips, damage, or signs of rust. The range hood fan and... cooler and freezer are operating properly, the shelves and door containers are secure and free of rust...

  8. 76 FR 72440 - Bottom Mount Combination Refrigerator-Freezers From Korea and Mexico; Scheduling of the Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-23

    ... refrigeration using compression technology, with all of the following characteristics: (1) The cabinet contains... interior plastic liner, (e) wiring, and (f) insulation; (2) any assembled external doors designed for use... metal shell, (b) an interior plastic liner, and (c) insulation; and (3) any assembled external drawers...

  9. KENNEDY SPACE CENTER, FLA. - The Minus Eighty Lab Freezer for ISS (MELFI), provided as Laboratory Support Equipment by the European Space Agency for the International Space Station, is seen in the Space Station Processing Facility. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

    NASA Image and Video Library

    2003-09-08

    KENNEDY SPACE CENTER, FLA. - The Minus Eighty Lab Freezer for ISS (MELFI), provided as Laboratory Support Equipment by the European Space Agency for the International Space Station, is seen in the Space Station Processing Facility. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

  10. KENNEDY SPACE CENTER, FLA. - After removing its cover, technicians look over the Minus Eighty Lab Freezer for ISS (MELFI), provided as Laboratory Support Equipment by the European Space Agency for the International Space Station. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

    NASA Image and Video Library

    2003-09-08

    KENNEDY SPACE CENTER, FLA. - After removing its cover, technicians look over the Minus Eighty Lab Freezer for ISS (MELFI), provided as Laboratory Support Equipment by the European Space Agency for the International Space Station. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

  11. KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, technicians remove the cover from the Minus Eighty Lab Freezer for ISS(MELFI) provided as Laboratory Support Equipment by the European Space Agency for the International Space Station. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

    NASA Image and Video Library

    2003-09-08

    KENNEDY SPACE CENTER, FLA. - In the Space Station Processing Facility, technicians remove the cover from the Minus Eighty Lab Freezer for ISS(MELFI) provided as Laboratory Support Equipment by the European Space Agency for the International Space Station. The lab will provide cooling and storage for reagents, samples and perishable materials in four insulated containers called dewars with independently selectable temperatures of -80°C, -26°C, and +4°C. It also will be used to transport samples to and from the station. The MELFI is planned for launch on the ULF-1 mission.

  12. Could cryopreserved human semen samples be stored at -80°C?

    PubMed Central

    Vaz, Carlos R; Lamim, Tamara; Salvador, Rafael A; Batschauer, Anna P B; Amaral, Vera Lucia L; Til, David

    2018-01-01

    Objective To evaluate storage time effects in cryopreserved human semen samples, kept in the freezer at a controlled temperature of -80°C, on sperm viability after thawing. Methods We used 20 semen samples. Each sample was cryopreserved in 10 fingers, which were divided into five groups: one group was kept in cryogenic canisters throughout the experiment(control), and four groups were kept in a VIP Ultra Low MDF-U76V- PE freezer, with the temperature set at -80°C, for 24, 48, 72 and 96 hours, respectively. After the exposure time, the samples were stored in cryogenic canisters after being thawed. The analyzed parameters were: motility, vitality and mitochondrial activity. Results After thawing, we noticed decreased sperm motility, vitality and mitochondrial activity, when comparing the tested groups with the control group, as well as a progressive reduction in the analyzed parameters between the times evaluated. Conclusions Cryopreservation of semen samples at -80°C is potentially harmful to sperm viability, causing damage when submitted to longer exposure times. PMID:29338138

  13. Application of transglutaminase and fermizyme for sensory quality improvement of pastry.

    PubMed

    Hozová, Bernadetta; Kukurová, Iveta; Dodok, Ladislav

    2003-06-01

    The objective of the model experiment was to find out the improving effect of two selected enzymes, transglutaminase (i) and fermizyme (ii), added at different concentrations of 4.5 mg and 7.5 mg/300 g flour (i) and 15 mg and 60 mg/300 g flour (ii) to the pastry dough on the quality of end products. The investigation was aimed to observe some changes of the sensory parameters (sensory profile) of pastry produced from the freezer-stored dough (-18 +/- 2 degrees C/0, 1, 7 and 14 days), namely shape (camber), odour, taste, crust colour (thickness/hardness), crumb elasticity (porosity, colour, hardness), adhesiveness to palate, etc. It has been ascertained that the sensory quality is favourably affected by the addition of the lower concentration of both enzymes: transglutaminase, 4.5 mg/300 g flour and fermizyme, 15 mg/300 g flour in comparison to the control without enzymes. The cambering ratio values and other sensory profile parameters of pastry gradually decreased during the freezer storage of dough. The best sensory evaluation of both kinds of pastry was achieved after a one-day storage of doughs.

  14. Design of evaporator of spacelab refrigerator/freezer

    NASA Technical Reports Server (NTRS)

    Hye, A.

    1985-01-01

    An Evaporator has been designed for NASA-Johnson Space Center Life Sciences to conduct experiments in Spacelab mission SLS-1 using different samples such as blood, urine, human tissues etc. Two units will fly - one as a Refrigerator (4 C) and the other as a Freezer (-22 C). The evaporator tube is dip brazed on a grooved flat plate. Aluminum heat sink is dip brazed on the other side of the plate. Freon R5O2 is pumped through the tube and air is circulated over the finned surface to transfer heat. As freon 5O2 is considered toxic, the whole freon tube is covered with an evaporator cover to contain any freon leakage to avoid exposure to crew members. This containment is under vacuum and this pressure is monitored along with the freon pressure to determine freon leakage so that necessary steps can be taken to stop contamination of the spacelab air. An stress analysis has been done and it is found to have adequate safety margin to meet the requirements of NASA safety and reliability standards.

  15. Effect of controlled and uncontrolled rate of cooling, prior to controlled rate of freezing, on motion characteristics and acrosomal integrity of cryopreserved ram spermatozoa.

    PubMed

    Joshi, Anil; Kumar, Davendra; Naqvi, S M K; Maurya, V P

    2008-12-01

    A programmable cell freezer provides ideal cryobiological conditions for controlled-rate cooling and freezing of ram spermatozoa. The purpose of this study was to investigate the effects of controlled (Group 1) and uncontrolled (Group 2) cooling conditions prior to programmable freezing of ram semen on post-thaw sperm motion characteristics and acrosomal integrity of ram spermatozoa. Semen samples of good initial motility obtained from adult Malpura rams were pooled, diluted to 1 × 10(9) spermatozoa per milliliter with Egg yolk-TEST-glycerol extender, and packaged in 0.25 mL straws. Straws representing Group 1 were cooled in a programmable cell freezer from 25°C to 5°C at the rate of -0.15°C per minute followed by a holding time of 2 h for equilibration, while straws of Group 2 were allowed to cool slowly up to 5°C and equilibrate for 2 h in the cold cabinet. After equilibration, straws of Group 2 were also loaded in the cell freezer for freezing straws of both the treatment groups simultaneously from 5°C to -125°C at the rate of -25°C per minute. Thawing of straws was done at 50°C for 10 s and the quality of frozen-thawed spermatozoa was objectively assessed by using sperm motility analyzer. Thawed samples were also evaluated for acrosomal integrity after staining the dried semen smears with Giemsa stain. The average post-thaw motility of straws was significantly higher (P < 0.05) in samples frozen after controlled cooling, compared with samples frozen after uncontrolled rate of cooling. The percent of spermatozoa with normal acrosome was also significantly (P < 0.05) higher in Group 1, compared to Group 2. The results indicate that controlled-rate cooling has a significant effect on post-thaw motility and acrosomal integrity of frozen-thawed ram spermatozoa, compared to uncontrolled-rate cooling prior to programmable freezing.

  16. Calcium silicate insulation structure

    DOEpatents

    Kollie, Thomas G.; Lauf, Robert J.

    1995-01-01

    An insulative structure including a powder-filled evacuated casing utilizes a quantity of finely divided synthetic calcium silicate having a relatively high surface area. The resultant structure-provides superior thermal insulating characteristics over a broad temperature range and is particularly well-suited as a panel for a refrigerator or freezer or the insulative barrier for a cooler or a insulated bottle.

  17. Effect of sample preparation method on sensory quality of cooked chicken breast fillets processed for food service

    USDA-ARS?s Scientific Manuscript database

    Chicken fillets (Pectoralis major) are one of popular items for food service. In the store, especially in fast food service stores, ready-to-cook meat products are commonly stored in freezers before use. The frozen meat can be cooked either directly from a frozen stage or after thawing. However, the...

  18. 77 FR 1474 - Decision and Order Granting a Waiver to Samsung From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-10

    ... DEPARTMENT OF ENERGY Office of Energy Efficiency and Renewable Energy [Case No. RF-018; Case No... Refrigerator and Refrigerator-Freezer Test Procedures AGENCY: Office of Energy Efficiency and Renewable Energy... characteristics. 10 CFR 430.27(b)(1)(iii). The Assistant Secretary for Energy Efficiency and Renewable Energy (the...

  19. 76 FR 33631 - Energy Conservation Program: Test Procedures for Walk-In Coolers and Walk-In Freezers

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-09

    ... a multiplex condensing system. U-factor means the heat transmission in a unit time through a unit... 550 08 19 or http://www.cen.eu/ . (1) DIN EN 13164:2009-02, (``DIN EN 13164''), Thermal insulation... 13165''), Thermal insulation products for buildings--Factory made rigid polyurethane foam (PUR) products...

  20. Identification of Viruses Present in Tissues Collected from Chickens with Hypoglycemia-Spiking Mortality Syndrome (H-SMS).

    USDA-ARS?s Scientific Manuscript database

    Tissues were collected, over a 10-year period, from broiler chickens diagnosed with severe H-SMS at the Georgia Poultry Lab, in Oakwood, GA. All samples were stored in tissue culture media, with antibiotics and 15% fetal bovine serum, in an ultra-cold freezer @ -80F. Specimens were homogenized,...

  1. Poster Puzzler Solution: Chill Out | Poster

    Cancer.gov

    A winner has emerged in the most recent Poster Puzzler contest! Congratulations are in order for Rose Bradley, secretary III, Cancer Research Technology Program. The current Poster Puzzler image shows the refrigerant condensers for the two story freezers in the Building 1073 repository, which are used to store samples at -20°C. Put simply, the condensers act like the outdoor

  2. Terrorist Financing: The 9/11 Commission Recommendation

    DTIC Science & Technology

    2005-02-25

    thing we didn’t do effectively is follow the money . That’s what we have to do.”6 While the goals of freezing terrorist funds and tracking them for...under President Bill Clinton, “There is a big ideological divide right now between the asset freezers and the people who want to follow the money as it

  3. Expedition 28 Crew Members remove samples from the JPM MELFI

    NASA Image and Video Library

    2011-07-08

    ISS028-E-014918 (8 July 2011) --- NASA astronauts Ron Garan (left) and Mike Fossum, both Expedition 28 flight engineers, remove samples from the General Laboratory Active Cryogenic ISS Experiment Refrigerator (GLACIER) and insert in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  4. Expedition 28 Crew Members remove samples from the JPM MELFI

    NASA Image and Video Library

    2011-07-08

    ISS028-E-014916 (8 July 2011) --- NASA astronauts Ron Garan (left) and Mike Fossum, both Expedition 28 flight engineers, remove samples from the General Laboratory Active Cryogenic ISS Experiment Refrigerator (GLACIER) and insert in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI-1) in the Kibo laboratory of the International Space Station.

  5. 77 FR 73415 - Approval for Manufacturing Authority; Foreign-Trade Zone 277; Sub-Zero, Inc. (Refrigerators and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-10

    ... Authority; Foreign-Trade Zone 277; Sub-Zero, Inc. (Refrigerators and Freezers); Goodyear, AZ Pursuant to its... Foreign Trade Zone, Inc., grantee of FTZ 277, has requested manufacturing authority on behalf of Sub-Zero... procedures within FTZ 277 on behalf of Sub-Zero, Inc., as described in the application and Federal Register...

  6. Cold temperature disinfestation of bagged flour

    USDA-ARS?s Scientific Manuscript database

    We conducted studies using a commercial freezer maintained at -17.8°C to determine the time needed to kill Tribolium castaneum eggs in a pallet of flour. Each bag weighed 22.7 kg, and there were 5 bags in each of 10 layers. The dimensions of the pallet were 109-cm wide by 132-cm long by 123-cm tall,...

  7. STS-42 Mission Specialist (MS) Hilmers reviews checklist in IML-1 module

    NASA Image and Video Library

    1992-01-30

    STS042-17-001 (22 Jan 1992) --- Astronaut David C. Hilmers, mission specialist, looks over a checklist at the Johnson Space Center refrigerator/freezer, in which perishable samples are stowed. The view gives an overall perspective of the science module -- heavily utilized for eight-days of scientific research supporting the International Microgravity Laboratory (IML-1) -- in Discovery's cargo bay.

  8. Gene activity test determines cold tolerance in Douglas-fir seedlings

    Treesearch

    Peter A. Balk; Diane L. Haase; Monique F. van Wordragen

    2008-01-01

    Forest tree nurseries rely on a tight scheduling of operations to be able to deliver vigorous seedlings to the planting site. Cooler or freezer storage is often used to maintain planting stock in an inactive condition and to ensure a plant supply for geographically diverse planting sites, which is a requirement for large-scale or internationally operating nurseries....

  9. 77 FR 75426 - Decision and Order Granting a Waiver to GE Appliances From the Department of Energy Residential...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-20

    ... alternate test procedures. 10 CFR 430.27(l). Waivers remain in effect pursuant to the provisions of 10 CFR... for products that employ these long-time or variable defrost control strategies, DOE is granting GE's... representations about the energy use of its refrigerator-freezer products for compliance, marketing, or other...

  10. Nutrition and Foods as Related to Space Flight

    NASA Technical Reports Server (NTRS)

    Lane, Helen W.; Smith, Scott M.; Bourland, Charles T.; Paloski, W. H. (Technical Monitor)

    1999-01-01

    U.S. space food development began with highly engineered foods that met rigid requirements imposed by the spacecraft design and short mission durations of the Mercury and Gemini programs. The lack of adequate bathroom facilities and limited food storage capacity promoted the development of low fiber diets to reduce fecal output. As missions lengthened, space food systems evolved, with the most basic design consideration always being the method of water supply. On the Apollo spacecraft, where water was abundant as a byproduct of fuel cell electricity generation, dehydrated food was used extensively. Such food has little advantage when water has to be transported to space to rehydrate it; therefore, more complex food systems were planned for Skylab, which used solar panels rather than fuel cells for electricity generation. The Skylab food system, the most advanced used in space to date, included freezers and refrigerators, increasing the palatability, variety, and nutritional value of the diet. On the Space Shuttle, power and weight constraints precluded the use of freezers, refrigerators, and microwave ovens. The availability of fuel cell by-product water was conducive to a shelf-stable food system with approximately half of the food dehydrated and the remainder made up of thermostabilized, irradiated, and intermediate-moisture foods.

  11. ‘HighChest’: An Augmented Freezer Designed for Smart Food Management and Promotion of Eco-Efficient Behaviour

    PubMed Central

    Bonaccorsi, Manuele; Betti, Stefano; Rateni, Giovanni; Esposito, Dario; Brischetto, Alessia; Marseglia, Marco; Dario, Paolo; Cavallo, Filippo

    2017-01-01

    This paper introduces HighChest, an innovative smart freezer designed to promote energy efficient behavior and the responsible use of food. Introducing a novel human–machine interface (HMI) design developed through assessment phases and a user involvement stage, HighChest is state of the art, featuring smart services that exploit embedded sensors and Internet of things functionalities, which enhance the local capabilities of the appliance. The industrial design thinking approach followed for the advanced HMI is intended to maximize the social impact of the food management service, enhancing both the user experience of the product and the user’s willingness to adopt eco- and energy-friendly behaviors. The sensor equipment realizes automatic recognition of food by learning from the users, as well as automatic localization inside the deposit space. Moreover, it provides monitoring of the appliance’s usage, avoiding temperature and humidity issues related to improper use. Experimental tests were conducted to evaluate the localization system, and the results showed 100% accuracy for weights greater or equal to 0.5 kg. Drifts due to the lid opening and prolonged usage time were also measured, to implement automatic reset corrections. PMID:28604609

  12. Cryopreservation of human sperm: efficacy and use of a new nitrogen-free controlled rate freezer versus liquid nitrogen vapour freezing.

    PubMed

    Creemers, E; Nijs, M; Vanheusden, E; Ombelet, W

    2011-12-01

    Preservation of spermatozoa is an important aspect of assisted reproductive medicine. The aim of this study was to investigate the efficacy and use of a recently developed liquid nitrogen and cryogen-free controlled rate freezer and this compared with the classical liquid nitrogen vapour freezing method for the cryopreservation of human spermatozoa. Ten patients entering the IVF programme donated semen samples for the study. Samples were analysed according to the World Health Organization guidelines. No significant difference in total sperm motility after freeze-thawing between the new technique and classical technique was demonstrated. The advantage of the new freezing technique is that it uses no liquid nitrogen during the freezing process, hence being safer to use and clean room compatible. Investment costs are higher for the apparatus but running costs are only 1% in comparison with classical liquid nitrogen freezing. In conclusion, post-thaw motility of samples frozen with the classical liquid nitrogen vapour technique was comparable with samples frozen with the new nitrogen-free freezing technique. This latter technique can thus be a very useful asset to the sperm cryopreservation laboratory. © 2011 Blackwell Verlag GmbH.

  13. NUTRITION w/Repository

    NASA Image and Video Library

    2009-06-06

    ISS020-E-007566 (6 June 2009) --- European Space Agency astronaut Frank De Winne, Expedition 20 flight engineer, prepares to put samples in the Minus Eighty Laboratory Freezer for ISS (MELFI) in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

  14. Immune Blood Sample Draw

    NASA Image and Video Library

    2012-04-26

    ISS030-E-257690 (26 April 2012) --- European Space Agency astronaut Andre Kuipers, Expedition 30 flight engineer, prepares for IMMUNE venous blood sample draws in the Columbus laboratory of the International Space Station. Following the blood draws, the samples were temporarily stowed in the Minus Eighty Laboratory Freezer for ISS 1 (MELFI-1) and later packed together with saliva samples on the Soyuz TMA-22 for return to Earth for analysis.

  15. How a Trip to the Freezer Can Help Children Learn Science

    ERIC Educational Resources Information Center

    Keogh, Brenda; Naylor, Stuart

    2011-01-01

    There are lots of exciting ways for children to learn science. Going on a journey is one of them. However, when going to distant places is not possible, a virtual journey may be the next best option. Ricky, one of the authors' puppets, helps children to make virtual journeys, and this can create an exciting context for them to raise questions and…

  16. NCI at Frederick Team Receives 2014 HHS Green Champions Award | Poster

    Cancer.gov

    A team of NCI and Leidos Biomedical Research employees at NCI at Frederick received the Energy and Fleet Management Award, one of the 2014 Department of Health and Human Services (HHS) Green Champions Awards, for comparing the costs and energy usage of two -80°C freezer technologies. This was the first scientific study to be jointly conducted by Leidos Biomedical Research’s

  17. 78 FR 62988 - Energy Conservation Program: Energy Conservation Standards for Certain Consumer Products and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-23

    ... wall, ceiling, and door insulation of R-25 for coolers and R-32 for freezers, but that glazed doors and....306(a)(3)) Section 2 of the AEMTCA added to EPCA a provision that the applicable walk-in insulation... insulation requirement were to apply. (42 U.S.C. 6313(f)(6)) This provision also states that, in support of...

  18. NUTRITION w/Repository

    NASA Image and Video Library

    2009-06-06

    ISS020-E-007577 (6 June 2009) --- European Space Agency astronaut Frank De Winne, Expedition 20 flight engineer, returns a dewar tray to the Minus Eighty Laboratory Freezer for ISS (MELFI) after inserting biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

  19. 10 CFR Appendix B1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... defined in HRF-1-1979 in cubic feet, times (2) an adjustment factor. 1.2 “Anti-sweat heater” means a... interior surfaces of the cabinet. 1.3 “Anti-sweat heater switch” means a user-controllable switch or user interface which modifies the activation or control of anti-sweat heaters. 1.4 “Automatic Defrost” means a...

  20. 10 CFR Appendix B1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... defined in HRF-1-1979 in cubic feet, times (2) an adjustment factor. 1.2“Anti-sweat heater” means a device... surfaces of the cabinet. 1.3“Anti-sweat heater switch” means a user-controllable switch or user interface which modifies the activation or control of anti-sweat heaters. 1.4“Automatic Defrost” means a system in...

  1. 10 CFR Appendix B1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... defined in HRF-1-1979 in cubic feet, times (2) an adjustment factor. 1.2“Anti-sweat heater” means a device... surfaces of the cabinet. 1.3“Anti-sweat heater switch” means a user-controllable switch or user interface which modifies the activation or control of anti-sweat heaters. 1.4“Automatic Defrost” means a system in...

  2. 10 CFR 431.304 - Uniform test method for the measurement of energy consumption of walk-in coolers and walk-in...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... machines. (b) Testing and Calculations. (1) [Reserved] (2) The R value shall be the 1/K factor multiplied by the thickness of the panel. (3) The K factor shall be based on ASTM C518 (incorporated by reference; see § 431.303). (4) For calculating the R value for freezers, the K factor of the foam at 20...

  3. 10 CFR 431.304 - Uniform test method for the measurement of energy consumption of walk-in coolers and walk-in...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... machines. (b) Testing and Calculations. (1) [Reserved] (2) The R value shall be the 1/K factor multiplied by the thickness of the panel. (3) The K factor shall be based on ASTM C518 (incorporated by reference; see § 431.303). (4) For calculating the R value for freezers, the K factor of the foam at 20...

  4. Mutagen Sensitivity, Apoptosis, and Polymorphism in DNA Repair as Measures of Prostate Cancer Risk

    DTIC Science & Technology

    2005-02-01

    blood tubes are immediately refrigerated and delivered on ice within 6 hours to the GCRC core facility at Georgetown University for processing . Case...is CLIA certified. There is a centrally monitored storage facility with -80’C freezers. The established recruitment and optimized sample processing in...controls. We have established the recruitment procedures, sample collection, processing , repository, and data management. This is a substantial effort

  5. Space Station galley design

    NASA Technical Reports Server (NTRS)

    Trabanino, Rudy; Murphy, George L.; Yakut, M. M.

    1986-01-01

    An Advanced Food Hardware System galley for the initial operating capability (IOC) Space Station is discussed. Space Station will employ food hardware items that have never been flown in space, such as a dishwasher, microwave oven, blender/mixer, bulk food and beverage dispensers, automated food inventory management, a trash compactor, and an advanced technology refrigerator/freezer. These new technologies and designs are described and the trades, design, development, and testing associated with each are summarized.

  6. Commander prepares glass columns for electrophoresis experiment

    NASA Technical Reports Server (NTRS)

    1982-01-01

    Commander Jack Lousma prepares on of the glass columns for the electrophoresis test in the middeck area of the Columbia. The experiment, deployed in an L-shaped mode in upper right corner, consists of the processing unit with glass columns in which the separation takes place; a camera (partially obscurred by Lousma's face) to document the process; and a cryogenic freezer to freeze and store the samples after separation.

  7. Fatty acids and plasmalogens of the phospholipids of the sperm membranes and their relation with the post-thaw quality of stallion spermatozoa.

    PubMed

    Macías García, B; González Fernández, L; Ortega Ferrusola, C; Morillo Rodríguez, A; Gallardo Bolaños, J M; Rodríguez Martinez, H; Tapia, J A; Morcuende, D; Peña, F J

    2011-03-15

    Fatty acids and plasmalogens were extracted from the phospholipids of the plasma membrane of stallion spermatozoa, to determine their relation with sperm quality after freezing and thawing. Sperm quality was rated using a quality index that combined the results of the analysis of sperm motility and velocity (CASA analysis), membrane status and mitochondrial membrane potential (flow cytometry) post thaw. Receiving operating system (ROC) curves were used to evaluate the value of specific lipid components of the sperm membrane herein studied as forecast of potential freezeability. From all parameters studied the ratio of percentage of C16 plasmalogens related to total phospholipids was the one with the better diagnostic value. For potentially bad freezers, the significant area under the ROC-curve was 0.74, with 75% sensitivity and 79.9% specificity for a cut off value of 26.9. Also the percentage of plasmalogens respect to total phospholipids gave good diagnostic value for bad freezers. On the other hand, the percentage of C18 fatty aldehydes related to total phospholipids of the sperm membrane properly forecasted freezeability with an area under the ROC curve of 0.70 with 70% sensitivity and 62.5% specificity for a cut off value of 0.32. Copyright © 2011 Elsevier Inc. All rights reserved.

  8. Preserving and vouchering butterflies and moths for large-scale museum-based molecular research

    PubMed Central

    Epstein, Samantha W.; Mitter, Kim; Hamilton, Chris A.; Plotkin, David; Mitter, Charles

    2016-01-01

    Butterflies and moths (Lepidoptera) comprise significant portions of the world’s natural history collections, but a standardized tissue preservation protocol for molecular research is largely lacking. Lepidoptera have traditionally been spread on mounting boards to display wing patterns and colors, which are often important for species identification. Many molecular phylogenetic studies have used legs from pinned specimens as the primary source for DNA in order to preserve a morphological voucher, but the amount of available tissue is often limited. Preserving an entire specimen in a cryogenic freezer is ideal for DNA preservation, but without an easily accessible voucher it can make specimen identification, verification, and morphological work difficult. Here we present a procedure that creates accessible and easily visualized “wing vouchers” of individual Lepidoptera specimens, and preserves the remainder of the insect in a cryogenic freezer for molecular research. Wings are preserved in protective holders so that both dorsal and ventral patterns and colors can be easily viewed without further damage. Our wing vouchering system has been implemented at the University of Maryland (AToL Lep Collection) and the University of Florida (Florida Museum of Natural History, McGuire Center of Lepidoptera and Biodiversity), which are among two of the largest Lepidoptera molecular collections in the world. PMID:27366654

  9. Hypocretin measurement: shelf age of radioimmunoassay kit, but not freezer time, influences assay variability.

    PubMed

    Keating, Glenda; Bliwise, Donald L; Saini, Prabhjyot; Rye, David B; Trotti, Lynn Marie

    2017-09-01

    The hypothalamic peptide hypocretin 1 (orexin A) may be assayed in cerebrospinal fluid to diagnose narcolepsy type 1. This testing is not commercially available, and factors contributing to assay variability have not previously been comprehensively explored. In the present study, cerebrospinal fluid hypocretin concentrations were determined in duplicate in 155 patient samples, across a range of sleep disorders. Intra-assay variability of these measures was analyzed. Inter-assay correlation between samples tested at Emory and at Stanford was high (r = 0.79, p < 0.0001). Intra-assay correlation between samples tested in duplicate in our center was also high (r = 0.88, p < 0.0001); intra-assay variability, expressed as the difference between values as a percentage of the higher value, was low at 9.4% (SD = 7.9%). Although both time the sample spent in the freezer (r = 0.16, p = 0.04) and age of the kit used for assay (t = 3.64, p = 0.0004) were significant predictors of intra-kit variability in univariate analyses, only age of kit was significant in multivariate linear regression (F = 4.93, p = 0.03). Age of radioimmunoassay kit affects intra-kit variability of measured hypocretin values, such that kits closer to expiration exhibit significantly more variability.

  10. Efficient long-term cryopreservation of pluripotent stem cells at −80 °C

    PubMed Central

    Yuan, Ye; Yang, Ying; Tian, Yuchen; Park, Jinkyu; Dai, Aihua; Roberts, R. Michael; Liu, Yang; Han, Xu

    2016-01-01

    Current long term cryopreservation of cell stocks routinely requires the use of liquid nitrogen (LN2), because commonly used cryopreservation media containing cell membrane permeating cryoprotectants are thermally unstable when frozen at higher storage temperatures, e.g. −80 °C. This instability leads to ice recrystallization, causing progressive loss of cell viability over time under the storage conditions provided by most laboratory deep freezers. The dependency on LN2 for cell storage significantly increases operational expense and raises issues related to impaired working efficiency and safety. Here we demonstrate that addition of Ficoll 70 to cryoprotectant solutions significantly improves system thermal stability at the working temperature (~−80 °C) of laboratory deep freezers. Moreover, a medium comprised of Ficoll 70 and dimethyl sulfoxide (DMSO) in presence or absence of fetal bovine serum (FBS) can provide reliable cryopreservation of various kinds of human and porcine pluripotent stem cells at −80 °C for periods that extend up to at least one year, with the post-thaw viability, plating efficiency, and full retention of pluripotent phenotype comparable to that achieved with LN2 storage. These results illustrate the practicability of a promising long-term cryopreservation method that completely eliminates the need for LN2. PMID:27694817

  11. Cold Stowage Flight Systems

    NASA Technical Reports Server (NTRS)

    Campana, Sharon

    2010-01-01

    The International Space Station (ISS) provides a test bed for researchers to perform science experiments in a variety of fields, including human research, life sciences, and space medicine. Many of the experiments being conducted today require science samples to be stored and transported in a temperature controlled environment. NASA provides several systems which aide researchers in preserving their science. On orbit systems provided by NASA include the Minus Eighty Laboratory freezer for ISS (MELFI), Microgravity Experiment Research Locker Incubator (MERLIN), and Glacier. These freezers use different technologies to provide rapid cooling and cold stowage at different temperature levels on board ISS. Systems available to researchers during transportation to and from ISS are MERLIN, Glacier, and Coldbag. Coldbag is a passive cold stowage system that uses phase change materials. Details of these current technologies will be provided along with operational experience gained to date. With shuttle retirement looming, NASA has protected the capability to provide a temperature controlled environment during transportation to and from the ISS with the use of Glacier and Coldbags, which are compatible with future commercial vehicles including SpaceX's Dragon Capsule, and Orbital s Cygnus vehicle. This paper will discuss the capability of the current cold stowage hardware and how it may continue to support NASA s mission on ISS and in future exploration missions.

  12. Creep and Sliding in Clay Slopes: Mutual Effects of Interlayer Swelling and Ice Jacking.

    DTIC Science & Technology

    1983-04-24

    calcite and feldspar constituents. Therefore a swelling clay with low diagenetic lithification (matrix forming) e fects. Therefore, the clay shows...were determined quantitatively (Tab. 2). The CEC depends mainly on the montmorillonite content and shows values up to 86 meq/lOOg, which Indicates...high montmorillonite clays. First freezing tests have been performed within a freezer. After freezing, three typical zones within the samples can be

  13. The Arrhenius Law and Storage of Food in a Freezer

    NASA Astrophysics Data System (ADS)

    Leenson, I. A.

    1999-04-01

    This article contains a brief review of some "unconventional" applications of the Arrhenius law. One such example is proposed as a problem concerning the shelf-life of frozen food (Italian pizza) at temperatures ranging from 0 to -18 °C. The effective activation energy (180 kJ/mole) calculated from the information presented by the manufacturer implies that the most probable mechanism of pizza deterioration on storage is enzyme and microbial destruction.

  14. NUTRITION w/Repository

    NASA Image and Video Library

    2009-06-06

    ISS020-E-007603 (7 June 2009) --- European Space Agency astronaut Frank De Winne, Expedition 20 flight engineer, removes a dewar tray from the Minus Eighty Laboratory Freezer for ISS (MELFI) in order to insert biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

  15. 10 CFR 431.304 - Uniform test method for the measurement of energy consumption of walk-in coolers and walk-in...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...-value of panels until January 1, 2015. (1) The R value shall be the 1/K factor multiplied by the thickness of the panel. (2) The K factor shall be based on ASTM C518 (incorporated by reference, see § 431.303). (3) For calculating the R value for freezers, the K factor of the foam at 20 degrees Fahrenheit...

  16. 10 CFR 431.304 - Uniform test method for the measurement of energy consumption of walk-in coolers and walk-in...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...-value of panels until January 1, 2015. (1) The R value shall be the 1/K factor multiplied by the thickness of the panel. (2) The K factor shall be based on ASTM C518 (incorporated by reference, see § 431.303). (3) For calculating the R value for freezers, the K factor of the foam at 20 degrees Fahrenheit...

  17. 10 CFR 431.304 - Uniform test method for the measurement of energy consumption of walk-in coolers and walk-in...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...-value of panels until January 1, 2015. (1) The R value shall be the 1/K factor multiplied by the thickness of the panel. (2) The K factor shall be based on ASTM C518 (incorporated by reference, see § 431.303). (3) For calculating the R value for freezers, the K factor of the foam at 20 degrees Fahrenheit...

  18. STS-40 crewmembers remove specimens from SLS-1 Rack 9 Refrigerator / Freezer

    NASA Image and Video Library

    1991-06-14

    STS040-202-033 (5-14 June 1991) --- A medium closeup scene shows astronaut James P. Bagian (left) and an unidentified crewmember (partially out of frame) looking at a vacant refrigerator in the Spacelab Life Sciences (SLS-1) module aboard the Earth-orbiting Space Shuttle Columbia. Following the detection of problems with the refrigerator, its contents were temporarily removed. This scene was photographed with a 35mm camera.

  19. 10 CFR Appendix A1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Electric Refrigerators and Electric...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... freezer compartment volume as defined in HRF-1-1979, in cubic feet. 1.3“Anti-sweat heater” means a device... operating characteristics. 1.7“Standard cycle” means the cycle type in which the anti-sweat heater control... unit under test. Defrost controls are to be operative and the anti-sweat heater switch is to be “on...

  20. 10 CFR Appendix B1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Freezers

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Standard as a revision of ANSI B38.1-1970. 1.2“Anti-sweat heater” means a device incorporated into the... characteristics. 1.5“Standard cycle” means the cycle type in which the anti-sweat heater switch, when provided, is... controls are to be operative and the anti-sweat heater switch is to be “on” during one test and “off...

  1. Skylab

    NASA Image and Video Library

    1970-01-01

    This photograph was taken during assembly of the bottom and upper floors of the Skylab Orbital Workshop (OWS). The OWS was divided into two major compartments. The lower level provided crew accommodations for sleeping, food preparation and consumption, hygiene, waste processing and disposal, and performance of certain experiments. The upper level consisted of a large work area and housed water storage tanks, a food freezer, storage vaults for film, scientific airlocks, mobility and stability experiment equipment, and other experimental equipment.

  2. Centrifuge for SLS-1

    NASA Image and Video Library

    1981-01-16

    S81-25565 (Feb 1981) --- Expected to be a busy item of flight hardware on the Spacelab Life Sciences (SLS-1) mission is this low-gravity centrifuge. To be flown onboard Columbia for STS-40, the centrifuge is able to simulate several gravity levels (0.5 g, 1.0 g, 1.5 g. and 2.0 g). Blood samples, taken during the flight, will be placed in the centrifuge, fixed for post flight analysis and transferred to a freezer.

  3. Effect of storage temperature on quality of light and full-fat ice cream.

    PubMed

    Buyck, J R; Baer, R J; Choi, J

    2011-05-01

    Ice cream quality is dependent on many factors including storage temperature. Currently, the industry standard for ice cream storage is -28.9 °C. Ice cream production costs may be decreased by increasing the temperature of the storage freezer, thus lowering energy costs. The first objective of this research was to evaluate the effect of 4 storage temperatures on the quality of commercial vanilla-flavored light and full-fat ice cream. Storage temperatures used were -45.6, -26.1, and -23.3 °C for the 3 treatments and -28.9 °C as the control or industry standard. Ice crystal sizes were analyzed by a cold-stage microscope and image analysis at 1, 19.5, and 39 wk of storage. Ice crystal size did not differ among the storage temperatures of light and full-fat ice creams at 19.5 or 39 wk. An increase in ice crystal size was observed between 19.5 and 39 wk for all storage temperatures except -45.6 °C. Coldness intensity, iciness, creaminess, and storage/stale off-flavor of the light and full-fat ice creams were evaluated at 39 wk of storage. Sensory evaluation indicated no difference among the different storage temperatures for light and full-fat ice creams. In a second study, light and full-fat ice creams were heat shocked by storing at -28.9 °C for 35 wk and then alternating between -23.3 and -12.2 °C every 24h for 4 wk. Heat-shocked ice creams were analyzed at 2 and 4 wk of storage for ice crystal size and were evaluated by the sensory panel. A difference in ice crystal size was observed for light and full-fat ice creams during heat-shock storage; however, sensory results indicated no differences. In summary, storage of light or full-fat vanilla-flavored ice creams at the temperatures used within this research did not affect quality of the ice creams. Therefore, ice cream manufacturers could conserve energy by increasing the temperature of freezers from -28.9 to -26.1 °C. Because freezers will typically fluctuate from the set temperature, usage of -26.1 °C allows for a safety factor, even though storage at -23.3 °C did not affect ice cream quality. Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  4. Wakata and Thirsk with MELFI in KIBO

    NASA Image and Video Library

    2009-06-15

    ISS020-E-010021 (15 June 2009) --- Canadian Space Agency astronaut Robert Thirsk (left) and Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, both Expedition 20 flight engineers, prepare to put samples in the Minus Eighty Laboratory Freezer for ISS (MELFI) in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight.

  5. New Forces Yet Undetermined: The Challenge of Biodefense

    DTIC Science & Technology

    2009-09-01

    processed. Smallpox virus was grown on the allantoic membrane of a fertile chicken egg. After examination of the membrane, it was a simple matter to place...Virologists have observed that it was common to put such vials in the back of a freezer and for old-fashioned-style labels to sometimes fall-off. Small- pox ...concern are naturally occurring. These include, for example, anthrax (in the Western United States), hanta virus (in New Mexico ), and tularemia (in Texas

  6. MELFI-3 Cold Box inspection

    NASA Image and Video Library

    2015-03-13

    ISS043e000724 (03/13/2015) --- ESA (European Space Agency) astronaut Samantha Cristoforetti, Expedition 43 flight engineer works daily on science and maintenance duties on board the International Space Station. She is inspecting the Minus Eighty-degree Laboratory Freezer called by the shorter title "MELFI-3 Cold Box inspection". Astronauts are trained for long periods at the Johnson Space Center and in Russia before their missions in space begin so that they are fully trained for these complex duties.

  7. Design, installation and operating experience of 20 photovoltaic medical refrigerator systems on four continents

    NASA Technical Reports Server (NTRS)

    Hein, G. F.

    1982-01-01

    The NASA Lewis Research Center in cooperation with the World Health Organization, U.S.A. I.D., the Pan American Health Organization and national government agencies in some developing countries sponsored the installation of twenty photovoltaic powered medical vaccine storage refrigerator-freezer (R/F) systems. The Solar Power Corporation was selected as the contractor to perform the design, development and installation of these twenty units. Solar Power's experiences are described herein.

  8. Fusible heat sink for EVA thermal control

    NASA Technical Reports Server (NTRS)

    Roebelen, G. J., Jr.

    1975-01-01

    The preliminary design and analysis of a heat sink system utilizing a phase change slurry material to be used eventually for astronaut cooling during manned space missions is described. During normal use, excess heat in the liquid cooling garment coolant is transferred to a reusable/regenerable fusible heat sink. Recharge is accomplished by disconnecting the heat sink from the liquid cooling garment and placing it in an on board freezer for simultaneous slurry refreeze and power supply electrical rechange.

  9. The "Food Polymer Science" approach to the practice of industrial R&D, leading to patent estates based on fundamental starch science and technology.

    PubMed

    Slade, Louise; Levine, Harry

    2018-04-13

    This article reviews the application of the "Food Polymer Science" approach to the practice of industrial R&D, leading to patent estates based on fundamental starch science and technology. The areas of patents and patented technologies reviewed here include: (a) soft-from-the-freezer ice creams and freezer-storage-stable frozen bread dough products, based on "cryostabilization technology" of frozen foods, utilizing commercial starch hydrolysis products (SHPs); (b) glassy-matrix encapsulation technology for flavors and other volatiles, based on structure-function relationships for commercial SHPs; (c) production of stabilized whole-grain wheat flours for biscuit products, based on the application of "solvent retention capacity" technology to develop flours with reduced damaged starch; (d) production of improved-quality, low-moisture cookies and crackers, based on pentosanase enzyme technology; (e) production of "baked-not-fried," chip-like, starch-based snack products, based on the use of commercial modified-starch ingredients with selected functionality; (f) accelerated staling of a starch-based food product from baked bread crumb, based on the kinetics of starch retrogradation, treated as a crystallization process for a partially crystalline glassy polymer system; and (g) a process for producing an enzyme-resistant starch, for use as a reduced-calorie flour replacer in a wide range of grain-based food products, including cookies, extruded expanded snacks, and breakfast cereals.

  10. A new use for long-term frozen brain tissue: Golgi impregnation

    PubMed Central

    Melendez-Ferro, Miguel; Perez-Costas, Emma; Roberts, Rosalinda C.

    2009-01-01

    The study of dendritic spine shape and number has become a standard in the analysis of synaptic transmission anomalies since a considerable number of neuropsychiatric and neurological diseases have their foundation in alterations in these structures. One of the best ways to study possible alterations of dendritic spines is the use of Golgi impregnation. Although usually the Golgi method implies the use of fresh or fixed tissue, here we report the use of Golgi-Cox for the staining of human and animal brain tissue kept frozen for long periods of time. We successfully applied the Golgi-Cox method to human brain tissue stored for up to 15 years in a freezer. The technique produced reliable and reproducible impregnation of dendrites and dendritic spines in different cortical areas. We also applied the same technique to rat brain frozen for up to one year, obtaining the same satisfactory results. The fact that Golgi-Cox can be successfully applied to this type of tissue adds a new value for hundreds of frozen human or animal brains kept in the freezers of the laboratories, that otherwise would not be useful for anything else. Researchers other than neuroanatomists, i.e. in fields such as biochemistry and molecular biology can also benefit from a simple and reliable technique that can be applied to tissue left from their primary experiments. PMID:18789970

  11. Prism adaptation in Parkinson disease: comparing reaching to walking and freezers to non-freezers.

    PubMed

    Nemanich, Samuel T; Earhart, Gammon M

    2015-08-01

    Visuomotor adaptation to gaze-shifting prism glasses requires recalibration of the relationship between sensory input and motor output. Healthy individuals flexibly adapt movement patterns to many external perturbations; however, individuals with cerebellar damage do not adapt movements to the same extent. People with Parkinson disease (PD) adapt normally, but exhibit reduced after-effects, which are negative movement errors following the removal of the prism glasses and are indicative of true spatial realignment. Walking is particularly affected in PD, and many individuals experience freezing of gait (FOG), an episodic interruption in walking, that is thought to have a distinct pathophysiology. Here, we examined how individuals with PD with (PD + FOG) and without (PD - FOG) FOG, along with healthy older adults, adapted both reaching and walking patterns to prism glasses. Participants completed a visually guided reaching and walking task with and without rightward-shifting prism glasses. All groups adapted at similar rates during reaching and during walking. However, overall walking adaptation rates were slower compared to reaching rates. The PD - FOG group showed smaller after-effects, particularly during walking, compared to PD + FOG, independent of adaptation magnitude. While FOG did not appear to affect characteristics of prism adaptation, these results support the idea that the distinct neural processes governing visuomotor adaptation and storage are differentially affected by basal ganglia dysfunction in PD.

  12. Cold-Chain Adaptability During Introduction of Inactivated Polio Vaccine in Bangladesh, 2015.

    PubMed

    Billah, Mallick M; Zaman, K; Estivariz, Concepcion F; Snider, Cynthia J; Anand, Abhijeet; Hampton, Lee M; Bari, Tajul I A; Russell, Kevin L; Chai, Shua J

    2017-07-01

    Introduction of inactivated polio vaccine creates challenges in maintaining the cold chain for vaccine storage and distribution. We evaluated the cold chain in 23 health facilities and 36 outreach vaccination sessions in 8 districts and cities of Bangladesh, using purposive sampling during August-October 2015. We interviewed immunization and cold-chain staff, assessed equipment, and recorded temperatures during vaccine storage and transportation. All health facilities had functioning refrigerators, and 96% had freezers. Temperature monitors were observed in all refrigerators and freezers but in only 14 of 66 vaccine transporters (21%). Recorders detected temperatures >8°C for >60 minutes in 5 of 23 refrigerators (22%), 3 of 6 cold boxes (50%) transporting vaccines from national to subnational depots, and 8 of 48 vaccine carriers (17%) used in outreach vaccination sites. Temperatures <2°C were detected in 4 of 19 cold boxes (21%) transporting vaccine from subnational depots to health facilities and 14 of 48 vaccine carriers (29%). Bangladesh has substantial cold-chain storage and transportation capacity after inactivated polio vaccine introduction, but temperature fluctuations during vaccine transport could cause vaccine potency loss that could go undetected. Bangladesh and other countries should strive to ensure consistent and sufficient cold-chain storage and monitor the cold chain during vaccine transportation at all levels. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America.

  13. Frost characteristics and heat transfer on a flat plate under freezer operating conditions: Part 1, Experimentation and correlations

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mao, Y.; Besant, R.W.; Chen, H.

    1999-07-01

    An experimental investigation of frost growth on a flat, cold surface supplied by subfreezing, turbulent, humid, parallel flow of air is presented. The operating conditions are typical of many commercial freezers. A test loop was constructed to perform the tests, and the frost height, frost mass concentration, and cold surface heat flux were measured using specially designed and calibrated instrumentation. Twenty tests were done for steady operating conditions, each starting with no initial frost accumulation, and were run for two to six hours giving 480 data samples. Measured results show that the frost characteristics differ significantly with frost growth datamore » taken previously for room temperature airflow. Depending on the temperature of the cold plate and the relative humidity of the subfreezing supply air, the frost could appear to be either smooth or rough. Smooth frost, which occurred at warmer plate temperatures and lower supply air relative humidities, gave rise to frost growth that was much thinner and denser than that for the rough, thick, low-density frost. Frost growth characteristics are correlated as a function of five independent variables (time, distance from the leading edge, cold plate temperature ratio, humidity ratio, and Reynolds number). These correlations are presented separately for the full data set, the rough frost data, and the smooth frost data.« less

  14. The influence of freezer storage of urine samples on the BONN-Risk-Index for calcium oxalate crystallization.

    PubMed

    Laube, Norbert; Zimmermann, Diana J

    2004-01-01

    This study was performed to quantify the effect of a 1-week freezer storage of urine on its calcium oxalate crystallization risk. Calcium oxalate is the most common urinary stone material observed in urolithiasis patients in western and affluent countries. The BONN-Risk-Index of calcium oxalate crystallization risk in human urine is determined from a crystallization experiment performed on untreated native urine samples. We tested the influence of a 1-week freezing on the BONN-Risk-Index value as well as the effect of the sample freezing on the urinary osmolality. In vitro crystallization experiments in 49 native urine samples from stone-forming and non-stone forming individuals were performed in order to determine their calcium oxalate crystallization risk according to the BONN-Risk-Index approach. Comparison of the results derived from original sample investigations with those obtained from the thawed aliquots by statistical evaluation shows that i) no significant deviation from linearity between both results exists and ii) both results are identical by statistical means. This is valid for both, the BONN-Risk-Index and the osmolality data. The differences in the BONN-Risk-Index results of both procedures of BONN-Risk-Index determination, however, exceed the clinically acceptable difference. Thus, determination of the urinary calcium oxalate crystallization risk from thawed urine samples cannot be recommended.

  15. Elevated Brain Harmane (1-methyl-9H-pyrido[3,4-b]indole) in Essential Tremor Cases vs. Controls

    PubMed Central

    Louis, Elan D.; Factor-Litvak, Pam; Liu, Xinhua; Vonsattel, Jean-Paul G.; Galecki, Monika; Jiang, Wendy; Zheng, Wei

    2013-01-01

    Background Harmane (1-methyl-9H-pyrido[3,4-β]indole), a potent neurotoxin that has tremor-producing properties in animal models, is present in many foods; Although we have demonstrated a difference in tissue harmane concentrations in ET cases vs. controls, all work to date has involved blood samples. Objectives We quantified harmane concentrations in human cerebellum, a brain region of particular pathogenic interest in essential tremor (ET), comparing ET to control brains. Methods Cerebellar cortex was snap frozen and stored at -80ºC in aliquots for biochemical analyses. Harmane concentration was assessed using high performance liquid chromatography. Results Geometric mean brain harmane concentrations (adjusted for postmortem interval [PMI] and freezer time) were higher in ET cases than controls: 1.0824 (95% confidence interval = 0.9405 – 1.2457) vs. 0.8037 (0.6967 – 0.9272), p = 0.004. Geometric mean of brain harmane concentrations (adjusting for PMI and freezer time) was highest in ET cases who reported other relatives with tremor (1.2005 [0.8712 – 1.6541]), intermediate in ET cases without family history (1.0312 ([0.8879 – 1.1976]), and both were significantly higher than controls (p= 0.02). Conclusions This study provides additional evidence of a possible etiological importance of this toxin in some cases of the human disease ET. PMID:23911942

  16. Elevated brain harmane (1-methyl-9H-pyrido[3,4-b]indole) in essential tremor cases vs. controls.

    PubMed

    Louis, Elan D; Factor-Litvak, Pam; Liu, Xinhua; Vonsattel, Jean-Paul G; Galecki, Monika; Jiang, Wendy; Zheng, Wei

    2013-09-01

    Harmane (1-methyl-9H-pyrido[3,4-β]indole), a potent neurotoxin that has tremor-producing properties in animal models, is present in many foods; although we have demonstrated a difference in tissue harmane concentrations in ET cases vs. controls, all work to date has involved blood samples. We quantified harmane concentrations in human cerebellum, a brain region of particular pathogenic interest in essential tremor (ET), comparing ET to control brains. Cerebellar cortex was snap frozen and stored at -80°C in aliquots for biochemical analyses. Harmane concentration was assessed using high performance liquid chromatography. Geometric mean brain harmane concentrations (adjusted for postmortem interval [PMI] and freezer time) were higher in ET cases than controls: 1.0824 (95% confidence interval=0.9405-1.2457) vs. 0.8037 (0.6967-0.9272), p=0.004. Geometric mean of brain harmane concentrations (adjusting for PMI and freezer time) was highest in ET cases who reported other relatives with tremor (1.2005 [0.8712-1.6541]), intermediate in ET cases without family history (1.0312 ([0.8879-1.1976]), and both were significantly higher than controls (p=0.02). This study provides additional evidence of a possible etiological importance of this toxin in some cases of the human disease ET. Copyright © 2013 Elsevier Inc. All rights reserved.

  17. Effect of sample storage temperature and buffer formulation on faecal immunochemical test haemoglobin measurements.

    PubMed

    Symonds, Erin L; Cole, Stephen R; Bastin, Dawn; Fraser, Robert Jl; Young, Graeme P

    2017-12-01

    Objectives Faecal immunochemical test accuracy may be adversely affected when samples are exposed to high temperatures. This study evaluated the effect of two sample collection buffer formulations (OC-Sensor, Eiken) and storage temperatures on faecal haemoglobin readings. Methods Faecal immunochemical test samples returned in a screening programme and with ≥10 µg Hb/g faeces in either the original or new formulation haemoglobin stabilizing buffer were stored in the freezer, refrigerator, or at room temperature (22℃-24℃), and reanalysed after 1-14 days. Samples in the new buffer were also reanalysed after storage at 35℃ and 50℃. Results were expressed as percentage of the initial concentration, and the number of days that levels were maintained to at least 80% was calculated. Results Haemoglobin concentrations were maintained above 80% of their initial concentration with both freezer and refrigerator storage, regardless of buffer formulation or storage duration. Stability at room temperature was significantly better in the new buffer, with haemoglobin remaining above 80% for 20 days compared with six days in the original buffer. Storage at 35℃ or 50℃ in the new buffer maintained haemoglobin above 80% for eight and two days, respectively. Conclusion The new formulation buffer has enhanced haemoglobin stabilizing properties when samples are exposed to temperatures greater than 22℃.

  18. A Comparison Study: The New Extended Shelf Life Isopropyl Ester PMR Technology versus The Traditional Methyl Ester PMR Approach

    NASA Technical Reports Server (NTRS)

    Alston, William B.; Scheiman, Daniel A.; Sivko, Gloria S.

    2005-01-01

    Polymerization of Monomeric Reactants (PMR) monomer solutions and carbon cloth prepregs of PMR II-50 and VCAP-75 were prepared using both the traditional limited shelf life methanol based PMR approach and a novel extended shelf life isopropanol based PMR approach. The methyl ester and isopropyl ester based PMR monomer solutions and PMR prepregs were aged for up to four years at freezer and room temperatures. The aging products formed were monitored using high pressure liquid chromatography (HPLC). The composite processing flow characteristics and volatile contents of the aged prepregs were also correlated versus room temperature storage time. Composite processing cycles were developed and six ply cloth laminates were fabricated with prepregs after various extended room temperature storage times. The composites were then evaluated for glass transition temperature (Tg), thermal decomposition temperature (Td), initial flexural strength (FS) and modulus (FM), long term (1000 hours at 316 C) thermal oxidative stability (TOS), and retention of FS and FM after 1000 hours aging at 316 C. The results for each ester system were comparable. Freezer storage was found to prevent the formation of aging products for both ester systems. Room temperature storage of the novel isopropyl ester system increased PMR monomer solution and PMR prepreg shelf life by at least an order of magnitude while maintaining composite properties.

  19. Space Station Freedom food management

    NASA Technical Reports Server (NTRS)

    Whitehurst, Troy N., Jr.; Bourland, Charles T.

    1992-01-01

    This paper summarizes the specification requirements for the Space Station Food System, and describes the system that is being designed and developed to meet those requirements. Space Station Freedom will provide a mix of frozen, refrigerated, rehydratable, and shelf stable foods. The crew will pre-select preferred foods from an approved list, to the extent that proper nutrition balance is maintained. A galley with freezers, refrigerators, trash compactor, and combination microwave and convection ovens will improve crew efficiency and productivity during the long Space Station Freedom (SSF) missions.

  20. Wakata and Thirsk with MELFI in KIBO

    NASA Image and Video Library

    2009-06-15

    ISS020-E-010028 (15 June 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 20 flight engineer, returns a dewar tray to the Minus Eighty Laboratory Freezer for ISS (MELFI) after inserting biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight. Canadian Space Agency astronaut Robert Thirsk, flight engineer, assisted Wakata.

  1. Vehicle-Snow Interaction: Modeling, Testing and Validation

    DTIC Science & Technology

    2009-10-12

    Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions...information if it does not display a currently valid OMB control number. 1 . REPORT DATE 12 OCT 2009 2. REPORT TYPE N/A 3. DATES COVERED - 4...surface – Snow temperature ~-6 C – Stored in a freezer ~-25 C • Microtribometer – – Temperature ~-10C – Pin sizes ( 1 /8”, 1 /4”, 3/8”, 1 /2”) – Force or

  2. Test and Evaluation of TRUST: Tools for Recognizing Useful Signals of Trustworthiness

    DTIC Science & Technology

    2016-04-01

    collection, the average mastoid signal was used to reference the data collected. • Four electrodes were placed beneath and to the side of each eye ...to record EOG activity. The data was used to remove eye -movement artifacts from the cortical activity (see below). Pre-Decision #1 Pre-Decision #2...into freezer vials, and stored in a -20 °F refrigerator. Within 1 week after collection, the samples were subsequently shipped (on dry ice) to the Air

  3. Meat Entree Item Production Guides Developed for Use in Ft. Lee Interim Central Food Preparation Facility

    DTIC Science & Technology

    1975-03-01

    serves 8). 10. Add cooked dough . (See topping procedure). 11. Cover, label and place in blast freezer. Totals Rotes: 1. Reheating...3. Gradually add cold water and mix only enough to form a soft dough . 4. Place dough on a lightly floured board, kneeding lightly about 1...minute or until dough Is smooth. 5. Roll out to a uniform thickness of 1/4 inch. 6. Cut into 2 i/4-inch diameter biscuits. 7. Bake for 15 minutes in

  4. Skylab

    NASA Image and Video Library

    1970-01-01

    This photograph was taken during installation of floor grids on the upper and lower floors inside the Skylab Orbital Workshop at the McDornell Douglas plant at Huntington Beach, California. The OWS was divided into two major compartments. The lower level provided crew accommodations for sleeping, food preparation and consumption, hygiene, waste processing and disposal, and performance of certain experiments. The upper level consisted of a large work area and housed water storage tanks, a food freezer, storage vaults for film, scientific airlocks, mobility and stability experiment equipment, and other experimental equipment.

  5. A fusible heat sink concept for extravehicular activity /EVA/ thermal control

    NASA Technical Reports Server (NTRS)

    Roebelen, G. J., Jr.

    1976-01-01

    This paper describes the preliminary design and analysis of a heat sink system, utilizing a phase change slurry material, to be used for astronaut and equipment cooling during manned space missions. During normal use, excess heat in the liquid cooling garment (LCG) coolant is transferred to a regenerable fusible heat sink. Recharge is accomplished by disconnecting the heat sink from the liquid cooling garment and placing it in an onboard freezer for simultaneous slurry refreeze and power supply recharge.

  6. Controlled-rate freezer cryopreservation of highly concentrated peripheral blood mononuclear cells results in higher cell yields and superior autologous T-cell stimulation for dendritic cell-based immunotherapy.

    PubMed

    Buhl, Timo; Legler, Tobias J; Rosenberger, Albert; Schardt, Anke; Schön, Michael P; Haenssle, Holger A

    2012-11-01

    Availability of large quantities of functionally effective dendritic cells (DC) represents one of the major challenges for immunotherapeutic trials against infectious or malignant diseases. Low numbers or insufficient T-cell activation of DC may result in premature termination of treatment and unsatisfying immune responses in clinical trials. Based on the notion that cryopreservation of monocytes is superior to cryopreservation of immature or mature DC in terms of resulting DC quantity and immuno-stimulatory capacity, we aimed to establish an optimized protocol for the cryopreservation of highly concentrated peripheral blood mononuclear cells (PBMC) for DC-based immunotherapy. Cryopreserved cell preparations were analyzed regarding quantitative recovery, viability, phenotype, and functional properties. In contrast to standard isopropyl alcohol (IPA) freezing, PBMC cryopreservation in an automated controlled-rate freezer (CRF) with subsequent thawing and differentiation resulted in significantly higher cell yields of immature and mature DC. Immature DC yields and total protein content after using CRF were comparable with results obtained with freshly prepared PBMC and exceeded results of standard IPA freezing by approximately 50 %. While differentiation markers, allogeneic T-cell stimulation, viability, and cytokine profiles were similar to DC from standard freezing procedures, DC generated from CRF-cryopreserved PBMC induced a significantly higher antigen-specific IFN-γ release from autologous effector T cells. In summary, automated controlled-rate freezing of highly concentrated PBMC represents an improved method for increasing DC yields and autologous T-cell stimulation.

  7. Liquid chromatography and ultra-performance liquid chromatography-mass spectrometry fingerprinting of human urine: sample stability under different handling and storage conditions for metabonomics studies.

    PubMed

    Gika, Helen G; Theodoridis, Georgios A; Wilson, Ian D

    2008-05-02

    Typically following collection biological samples are kept in a freezer for periods ranging from a few days to several months before analysis. Experience has shown that in LC-MS-based metabonomics research the best analytical practice is to store samples as these are collected, complete the sample set and analyse it in a single run. However, this approach is prudent only if the samples stored in the refrigerator or in the freezer are stable. Another important issue is the stability of the samples following the freeze-thaw process. To investigate these matters urine samples were collected from 6 male volunteers and analysed by LC-MS and ultra-performance liquid chromatography (UPLC)-MS [in both positive and negative electrospray ionization (ESI)] on the day of collection or at intervals of up to 6 months storage at -20 degrees C and -80 degrees C. Other sets of these samples underwent a series of up to nine freeze-thaw cycles. The stability of samples kept at 4 degrees C in an autosampler for up to 6 days was also assessed, with clear differences appearing after 48h. Data was analysed using multivariate statistical analysis (principal component analysis). The results show that sample storage at both -20 and -80 degrees C appeared to ensure sample stability. Similarly up to nine freeze thaw cycles were without any apparent effect on the profile.

  8. Crohn's disease and early exposure to domestic refrigeration.

    PubMed

    Malekzadeh, Fatemeh; Alberti, Corinne; Nouraei, Mehdi; Vahedi, Homayoon; Zaccaria, Isabelle; Meinzer, Ulrich; Nasseri-Moghaddam, Siavosh; Sotoudehmanesh, Rasoul; Momenzadeh, Sara; Khaleghnejad, Reza; Rashtak, Shahrooz; Olfati, Golrokh; Malekzadeh, Reza; Hugot, Jean-Pierre

    2009-01-01

    Environmental risk factors playing a causative role in Crohn's Disease (CD) remain largely unknown. Recently, it has been suggested that refrigerated food could be involved in disease development. We thus conducted a pilot case control study to explore the association of CD with the exposure to domestic refrigeration in childhood. Using a standard questionnaire we interviewed 199 CD cases and 207 age-matched patients with irritable bowel syndrome (IBS) as controls. Cases and controls were followed by the same gastroenterologists of tertiary referral clinics in Tehran, Iran. The questionnaire focused on the date of the first acquisition of home refrigerator and freezer. Data were analysed by a multivariate logistic model. The current age was in average 34 years in CD cases and the percentage of females in the case and control groups were respectively 48.3% and 63.7%. Patients were exposed earlier than controls to the refrigerator (X2 = 9.9, df = 3, P = 0.04) and refrigerator exposure at birth was found to be a risk factor for CD (OR = 2.08 (95% CI: 1.01-4.29), P = 0.05). Comparable results were obtained looking for the exposure to freezer at home. Finally, among the other recorded items reflecting the hygiene and comfort at home, we also found personal television, car and washing machine associated with CD. This study supports the opinion that CD is associated with exposure to domestic refrigeration, among other household factors, during childhood.

  9. Crohn's Disease and Early Exposure to Domestic Refrigeration

    PubMed Central

    Malekzadeh, Fatemeh; Alberti, Corinne; Nouraei, Mehdi; Vahedi, Homayoon; Zaccaria, Isabelle; Meinzer, Ulrich; Nasseri-Moghaddam, Siavosh; Sotoudehmanesh, Rasoul; Momenzadeh, Sara; Khaleghnejad, Reza; Rashtak, Shahrooz; Olfati, Golrokh; Malekzadeh, Reza; Hugot, Jean-Pierre

    2009-01-01

    Background Environmental risk factors playing a causative role in Crohn's Disease (CD) remain largely unknown. Recently, it has been suggested that refrigerated food could be involved in disease development. We thus conducted a pilot case control study to explore the association of CD with the exposure to domestic refrigeration in childhood. Methodology/Principal Findings Using a standard questionnaire we interviewed 199 CD cases and 207 age-matched patients with irritable bowel syndrome (IBS) as controls. Cases and controls were followed by the same gastroenterologists of tertiary referral clinics in Tehran, Iran. The questionnaire focused on the date of the first acquisition of home refrigerator and freezer. Data were analysed by a multivariate logistic model. The current age was in average 34 years in CD cases and the percentage of females in the case and control groups were respectively 48.3% and 63.7%. Patients were exposed earlier than controls to the refrigerator (X2 = 9.9, df = 3, P = 0.04) and refrigerator exposure at birth was found to be a risk factor for CD (OR = 2.08 (95% CI: 1.01–4.29), P = 0.05). Comparable results were obtained looking for the exposure to freezer at home. Finally, among the other recorded items reflecting the hygiene and comfort at home, we also found personal television, car and washing machine associated with CD. Conclusion This study supports the opinion that CD is associated with exposure to domestic refrigeration, among other household factors, during childhood. PMID:19177167

  10. Comparison between mechanical freezer and conventional freezing using liquid nitrogen in normozoospermia.

    PubMed

    Rahana, A R; Ng, S P; Leong, C F; Rahimah, M D

    2011-10-01

    This study evaluated the effect of human semen cryopreservation using an ultra-low temperature technique with a mechanical freezer at -85°C as an alternative method to the conventional liquid nitrogen technique at -196°C. This was a prospective experimental study conducted in the Medically Assisted Conception unit, Department of Obstetrics and Gynaecology, National University Hospital, Malaysia from January 1, 2006 to April 30, 2007. All normozoospermic semen samples were included in the study. The concentration, motility and percentage of intact DNA of each semen sample were assessed before and after freezing and thawing on Days 7 and 30 post freezing. Sperm cryopreservation at -85°C was comparable to the conventional liquid nitrogen technique for a period of up to 30 days in a normozoospermic sample. There was no statistical difference in concentration (Day 7 p-value is 0.1, Day 30 p-value is 0.2), motility (Day 7 p-value is 0.9, Day 30 p-value is 0.5) and proportion of intact DNA (Day 7 p-value is 0.1, Day 30 p-value is 0.2) between the ultra-low temperature technique and conventional liquid nitrogen cryopreservation at Days 7 and 30 post thawing. This study clearly demonstrates that short-term storage of sperm at -85°C could be a viable alternative to conventional liquid nitrogen cryopreservation at -196°C due to their comparable post-thaw results.

  11. Safe and efficient method for cryopreservation of human induced pluripotent stem cell-derived neural stem and progenitor cells by a programmed freezer with a magnetic field.

    PubMed

    Nishiyama, Yuichiro; Iwanami, Akio; Kohyama, Jun; Itakura, Go; Kawabata, Soya; Sugai, Keiko; Nishimura, Soraya; Kashiwagi, Rei; Yasutake, Kaori; Isoda, Miho; Matsumoto, Morio; Nakamura, Masaya; Okano, Hideyuki

    2016-06-01

    Stem cells represent a potential cellular resource in the development of regenerative medicine approaches to the treatment of pathologies in which specific cells are degenerated or damaged by genetic abnormality, disease, or injury. Securing sufficient supplies of cells suited to the demands of cell transplantation, however, remains challenging, and the establishment of safe and efficient cell banking procedures is an important goal. Cryopreservation allows the storage of stem cells for prolonged time periods while maintaining them in adequate condition for use in clinical settings. Conventional cryopreservation systems include slow-freezing and vitrification both have advantages and disadvantages in terms of cell viability and/or scalability. In the present study, we developed an advanced slow-freezing technique using a programmed freezer with a magnetic field called Cells Alive System (CAS) and examined its effectiveness on human induced pluripotent stem cell-derived neural stem/progenitor cells (hiPSC-NS/PCs). This system significantly increased cell viability after thawing and had less impact on cellular proliferation and differentiation. We further found that frozen-thawed hiPSC-NS/PCs were comparable with non-frozen ones at the transcriptome level. Given these findings, we suggest that the CAS is useful for hiPSC-NS/PCs banking for clinical uses involving neural disorders and may open new avenues for future regenerative medicine. Copyright © 2015 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

  12. Transient Air Infiltration/Exfiltration in Walk-In Coolers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Faramarzi, Ramin; Navaz, H. K.; Kamensky, K.

    Walk-in coolers are room-sized, insulated, and refrigerated compartments for food product storage. Walk-ins have areas equal or below 280 m2 (3,000 ft2), and are classified either as coolers operating above 0 degrees C (32 degrees F) (medium-temperature) to store fresh fruit, vegetables, and dairy products, or freezers that operate below 0 degrees C (32 degrees F) (low-temperature) to meet health and safety standards of frozen food products. Walk-ins are typically found in restaurants as well as small- and medium-to-large grocery stores or supermarkets.

  13. KSC-2009-4560

    NASA Image and Video Library

    2009-08-09

    CAPE CANAVERAL, Fla. – On Launch Pad 39A, the payload ground-handling mechanism moves back after placing the multi-purpose logistics module Leonardo in space shuttle Discovery's payload bay. Leonardo is the primary payload on Discovery's STS-128 mission to the International Space Station. Beneath the module is the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery will deliver 33,000 pounds of equipment to the station, including science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch is targeted for late August. Photo credit: NASA/Jack Pfaller

  14. Wakata and Thirsk with MELFI in KIBO

    NASA Image and Video Library

    2009-06-15

    ISS020-E-010025 (15 June 2009) --- Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata, Expedition 20 flight engineer, removes a dewar tray from the Minus Eighty Laboratory Freezer for ISS (MELFI) in order to insert biological samples into the trays in the Kibo laboratory of the International Space Station. Samples were taken as part of the Nutritional Status Assessment (Nutrition) with Repository experiment, a study done by NASA to date of human physiologic changes during long-duration spaceflight. Canadian Space Agency astronaut Robert Thirsk, flight engineer, assisted Wakata.

  15. Crew appliance study

    NASA Technical Reports Server (NTRS)

    Proctor, B. W.; Reysa, R. P.; Russell, D. J.

    1975-01-01

    Viable crew appliance concepts were identified by means of a thorough literature search. Studies were made of the food management, personal hygiene, housekeeping, and off-duty habitability functions to determine which concepts best satisfy the Space Shuttle Orbiter and Modular Space Station mission requirements. Models of selected appliance concepts not currently included in the generalized environmental-thermal control and life support systems computer program were developed and validated. Development plans of selected concepts were generated for future reference. A shuttle freezer conceptual design was developed and a test support activity was provided for regenerative environmental control life support subsystems.

  16. Interior View of the Orbital Workshop

    NASA Technical Reports Server (NTRS)

    1972-01-01

    This photograph is an interior view of the Orbital Workshop (OWS) upper level looking from the airlock hatch, showing the octagonal opening that separated the workshop's two levels. The trash airlock can be seen at center. The lower level of the OWS provided crew accommodations for sleeping, food preparation and consumption, hygiene, waste processing and disposal, and performance of certain experiments. The upper level consisted of a large work area and housed water storage tanks, a food freezer, storage vaults for film, scientific airlocks, mobility and stability experiment equipment, and other experimental equipment.

  17. Skylab Medical Experiments Altitude Test /SMEAT/ facility design and operation.

    NASA Technical Reports Server (NTRS)

    Hinners, A. H., Jr.; Correale, J. V.

    1973-01-01

    This paper presents the design approaches and test facility operation methods used to successfully accomplish a 56-day test for Skylab to permit evaluation of selected Skylab medical experiments in a ground test simulation of the Skylab environment with an astronaut crew. The systems designed for this test include the two-gas environmental control system, the fire suppression and detection system, equipment transfer lock, ground support equipment, safety systems, potable water system, waste management system, lighting and power system, television monitoring, communications and recreation systems, and food freezer.

  18. Variability of alpha-tocopherol values associated with procurement, storage, and freezing of equine serum and plasma samples.

    PubMed

    Craig, A M; Blythe, L L; Rowe, K E; Lassen, E D; Barrington, R; Walker, K C

    1992-12-01

    Recent evidence concerning the pathogenesis of equine degenerative myeloencephalopathy indicated that low blood alpha-tocopherol values are a factor in the disease process. Variables that could be introduced by a veterinarian procuring, transporting, or storing samples were evaluated for effects on alpha-tocopherol concentration in equine blood. These variables included temperature; light; exposure to the rubber stopper of the evacuated blood collection tube; hemolysis; duration of freezing time, with and without nitrogen blanketing; and repeated freeze/thaw cycles. It was found that hemolysis caused the greatest change in high-performance liquid chromatography-measured serum alpha-tocopherol values, with mean decrease of 33% (P < 0.001). Lesser, but significant (P < 0.01) changes in serum alpha-tocopherol values were an approximate 10% decrease when refrigerated blood was left in contact with the red rubber stopper of the blood collection tube for 72 hours and an approximate 5% increase when blood was stored at 20 to 25 C (room temperature) for 72 hours. Repeated freeze/thaw cycles resulted in a significant (P < 0.05) 3% decrease in alpha-tocopherol values in heparinized plasma by the third thawing cycle. Freezer storage for a 3-month period without nitrogen blanketing resulted in slight (2%) decrease in mean serum alpha-tocopherol values, whereas values in serum stored for an identical period under nitrogen blanketing did not change. A significant (P < 0.001) mean decrease (10.3%) in alpha-tocopherol values was associated with freezer (-16 C) storage of nitrogen blanketed serum for 6 months.(ABSTRACT TRUNCATED AT 250 WORDS)

  19. Random and independent sampling of endogenous tryptic peptides from normal human EDTA plasma by liquid chromatography micro electrospray ionization and tandem mass spectrometry.

    PubMed

    Dufresne, Jaimie; Florentinus-Mefailoski, Angelique; Ajambo, Juliet; Ferwa, Ammara; Bowden, Peter; Marshall, John

    2017-01-01

    Normal human EDTA plasma samples were collected on ice, processed ice cold, and stored in a freezer at - 80 °C prior to experiments. Plasma test samples from the - 80 °C freezer were thawed on ice or intentionally warmed to room temperature. Protein content was measured by CBBR binding and the release of alcohol soluble amines by the Cd ninhydrin assay. Plasma peptides released over time were collected over C18 for random and independent sampling by liquid chromatography micro electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS) and correlated with X!TANDEM. Fully tryptic peptides by X!TANDEM returned a similar set of proteins, but was more computationally efficient, than "no enzyme" correlations. Plasma samples maintained on ice, or ice with a cocktail of protease inhibitors, showed lower background amounts of plasma peptides compared to samples incubated at room temperature. Regression analysis indicated that warming plasma to room temperature, versus ice cold, resulted in a ~ twofold increase in the frequency of peptide identification over hours-days of incubation at room temperature. The type I error rate of the protein identification from the X!TANDEM algorithm combined was estimated to be low compared to a null model of computer generated random MS/MS spectra. The peptides of human plasma were identified and quantified with low error rates by random and independent sampling that revealed 1000s of peptides from hundreds of human plasma proteins from endogenous tryptic peptides.

  20. Food safety hazards lurk in the kitchens of young adults.

    PubMed

    Byrd-Bredbenner, Carol; Maurer, Jaclyn; Wheatley, Virginia; Cottone, Ellen; Clancy, Michele

    2007-04-01

    Food mishandling in home kitchens likely causes a significant amount of foodborne disease; however, little is known about the food safety hazards lurking in home kitchens. The purposes of this study were to audit the kitchens of young adults with education beyond high school to identify food safety problems and develop recommendations for education efforts. Researchers developed a criterion-referenced home kitchen observation instrument to assess compliance of home food storage and rotation practices (e.g., temperature), sanitation and chemical storage, and general kitchen condition (e.g., infestation) with recommended practices. The instrument contained seven scales: Kitchen Cleanliness (eight items), Appliance Cleanliness (three items), Cleaning Supplies Availability (eight items), Temperatures (Food Thermometer Access & Refrigerator/Freezer Temperatures) (five items), Cold Food Storage (seven items), Dry Food Storage (eight items), and Poisons Storage (two items). Descriptive statistics were conducted to describe the study population, as a whole, and by gender. A total of 154 young adults (mean age, 20.7+/- 1.3 SD) enrolled in a northeastern university participated. Participants scored 70% or higher on Poisons Storage, Dry Food Storage, Kitchen Cleanliness, and Cleaning Supplies Availability scales but less than 60% on the Appliance Cleanliness and Cold Food Storage scales. Performance was lowest on the Temperatures scale. Females scored significantly higher than males on the Kitchen Cleanliness and Cleaning Supply Availability scales. Average refrigerator and freezer temperatures were higher than recommendations. Food safety education targeted at this young adult population needs to evolve into focused messages pertaining to the key food safety violations in this population.

  1. Brain activation underlying turning in Parkinson’s disease patients with and without freezing of gait: a virtual reality fMRI study

    PubMed Central

    Gilat, Moran; Shine, James M; Walton, Courtney C; O’Callaghan, Claire; Hall, Julie M; Lewis, Simon J G

    2015-01-01

    Background: Freezing of gait is a debilitating symptom affecting many patients with Parkinson’s disease (PD), causing severe immobility and decreased quality of life. Turning is known to be the most common trigger for freezing and also causes the highest rates of falls. However, the pathophysiological basis for these effects is not well understood. Methods: This study used a virtual reality paradigm in combination with functional magnetic resonance imaging to explore the neural correlates underlying turning in 17 PD patients with freezing of gait (FOG) and 10 PD patients without FOG while off their dopaminergic medication. Participants used foot pedals to navigate a virtual environment, which allowed for blood oxygen level-dependent (BOLD) responses and footstep latencies to be compared between periods of straight “walking” and periods of turning through 90°. BOLD data were then analyzed using a mixed effects analysis. Results: Within group similarities revealed that overall, PD patients with freezing relied heavily on cortical control to enable effective stepping with increased visual cortex activation during turning. Between groups differences showed that when turning, patients with freezing preferentially activated inferior frontal regions that have been implicated in the recruitment of a putative stopping network. In addition, freezers failed to activate premotor and superior parietal cortices. Finally, increased task-based functional connectivity was found in subcortical regions associated with gait and stopping within the freezers group during turning. Conclusions: These findings suggest that an increased propensity towards stopping in combination with reduced sensorimotor integration may underlie the neurobiology of freezing of gait during turning. PMID:28725687

  2. Sperm Flagellum Volume Determines Freezability in Red Deer Spermatozoa

    PubMed Central

    Ros-Santaella, José Luis; Domínguez-Rebolledo, Álvaro Efrén; Garde, José Julián

    2014-01-01

    The factors affecting the inter-individual differences in sperm freezability is a major line of research in spermatology. Poor sperm freezability is mainly characterised by a low sperm velocity, which in turn is associated with low fertility rates in most animal species. Studies concerning the implications of sperm morphometry on freezability are quite limited, and most of them are based on sperm head size regardless of the structural parts of the flagellum, which provides sperm motility. Here, for the first time, we determined the volumes of the flagellum structures in fresh epididymal red deer spermatozoa using a stereological method under phase contrast microscopy. Sperm samples from thirty-three stags were frozen and classified as good freezers (GF) or bad freezers (BF) at two hours post-thawing using three sperm kinetic parameters which are strongly correlated with fertility in this species. Fourteen stags were clearly identified as GF, whereas nineteen were BF. No significant difference in sperm head size between the two groups was found. On the contrary, the GF exhibited a lower principal piece volume than the BF (6.13 µm3 vs 6.61 µm3, respectively, p = 0.006). The volume of the flagellum structures showed a strong negative relationship with post-thawing sperm velocity. For instance, the volume of the sperm principal piece was negatively correlated with sperm velocity at two hours post-thawing (r = −0.60; p<0.001). Our results clearly show that a higher volume of the sperm principal piece results in poor freezability, and highlights the key role of flagellum size in sperm cryopreservation success. PMID:25380133

  3. High-Throughput Cryopreservation of Plant Cell Cultures for Functional Genomics

    PubMed Central

    Ogawa, Yoichi; Sakurai, Nozomu; Oikawa, Akira; Kai, Kosuke; Morishita, Yoshihiko; Mori, Kumiko; Moriya, Kanami; Fujii, Fumiko; Aoki, Koh; Suzuki, Hideyuki; Ohta, Daisaku; Saito, Kazuki; Shibata, Daisuke

    2012-01-01

    Suspension-cultured cell lines from plant species are useful for genetic engineering. However, maintenance of these lines is laborious, involves routine subculturing and hampers wider use of transgenic lines, especially when many lines are required for a high-throughput functional genomics application. Cryopreservation of these lines may reduce the need for subculturing. Here, we established a simple protocol for cryopreservation of cell lines from five commonly used plant species, Arabidopsis thaliana, Daucus carota, Lotus japonicus, Nicotiana tabacum and Oryza sativa. The LSP solution (2 M glycerol, 0.4 M sucrose and 86.9 mM proline) protected cells from damage during freezing and was only mildly toxic to cells kept at room temperature for at least 2 h. More than 100 samples were processed for freezing simultaneously. Initially, we determined the conditions for cryopreservation using a programmable freezer; we then developed a modified simple protocol that did not require a programmable freezer. In the simple protocol, a thick expanded polystyrene (EPS) container containing the vials with the cell–LSP solution mixtures was kept at −30°C for 6 h to cool the cells slowly (pre-freezing); samples from the EPS containers were then plunged into liquid nitrogen before long-term storage. Transgenic Arabidopsis cells were subjected to cryopreservation, thawed and then re-grown in culture; transcriptome and metabolome analyses indicated that there was no significant difference in gene expression or metabolism between cryopreserved cells and control cells. The simplicity of the protocol will accelerate the pace of research in functional plant genomics. PMID:22437846

  4. Oxytocin Levels in Community-Collected Saliva Samples Transported by Dry Versus Wet Ice.

    PubMed

    Howland, Lois C; Pickler, Rita H; Sullenbarger, Brent A; Connelly, Cynthia D

    2018-01-01

    Oxytocin (OT), a neuropeptide produced primarily in the hypothalamus, is associated with both critical physiological and psychological processes, particularly stress and feelings of affiliation. Increasingly, researchers are seeking ways to reliably incorporate OT as an outcome biomarker in clinical research. Previously, OT levels were measured in plasma or urine. Recently, researchers have measured this biomarker in saliva, particularly when conducting research in clinical and community settings. In spite of increased interest in the use of salivary OT in clinical research, procedures for handling, transport, and analysis of specimens vary. It is not known if significant OT protein degradation occurs if samples are initially transported on wet ice before being frozen. The aim of this study is to evaluate the effect of transport media (wet vs. dry ice) on OT levels derived from saliva collected from 12 postpartum women residing in the community. Saliva collected from each participant was divided between two microcentrifuge tubes (MIDSCI, Valley Park, MO), one placed on wet ice and one on dry ice for transport from the participant's home to the laboratory freezer. Time from collection to storage freezer was recorded. Laboratory personnel, blinded to method of transport, batch processed the samples. No significant differences in OT levels were found by transport method. Despite large interperson variations in OT levels, there were negligible intraperson variations. Although further research is required to identify factors (including transport time) related to interperson variation, this study supports the use of wet ice as a means of transporting salivary OT specimens in community-based research.

  5. [The association between socioeconomic indicators andadolescents'physical activity and health-related fitness].

    PubMed

    Constantino-Coledam, Diogo H; Ferraiol, Philippe Fanelli; Arruda, Gustavo Aires de; Pires-Júnior, Raymundo; Teixeira, Marcio; Greca, João Paulo de Aguiar; Oliveira, Arli Ramos de

    2013-01-01

    This study was aimed at analysing the association between socioeconomic indicators and adolescents' physical activity and health-related fitness. The study involved 716 adolescents from both genders whose age ranged from 10 to 18 years-old (46.8% male) who answered a questionnaire for estimating their habitual physical activity, socioeconomic status; two health-related physical fitness tests were also performed. The socioeconomic indicators analysed concerned their parents' educational level and the number of bathrooms, TVs, cars, housemaids, refrigerators and freezers in their homes. A positive association was found between paternal education (PR=1.61 (range 1.27-2.10) and 1.41 (1.10-1.83)) and housemaids (PR=1.97 (1.04-3.81) and 1.92 (1.05-3.52)) with recommended physical activity and leisure time physical activity, respectively. The number of cars (PR=1.48: 1.02-2.19) and freezers (PR=1.88: 1.12-3.18) was positively associated with leisure time physical activity and the number of TVs negatively so (PR=0.75: 0.63-0.89). The number of TVs (PR=0.80: 0.67-0.96) and cars (PR=0.70: 0.55-0.89) was negatively associated with cardiorespiratory fitness whilst paternal education (PR=1.17: 1.00-1.37) and the number of bathrooms in the home (PR=1.25: 1.02-1.54) were positively associated with muscular strength. Physical activity and health-related physical fitness were associated with socioeconomic status. However, such association depended on the socioeconomic indicator being analysed. Caution should be taken when analysing studies which use different socioeconomic indicators.

  6. Design of a blood-freezing system for leukemia research

    NASA Technical Reports Server (NTRS)

    Williams, T. E.; Cygnarowicz, T. A.

    1978-01-01

    Leukemia research involves the use of cryogenic freezing and storage equipment. In a program being carried out at the National Cancer Institute (NCI), bone marrow (white blood cells) was frozen using a standard cryogenic biological freezer. With this system, it is difficult to maintain the desired rate of freezing and repeatability from sample to sample. A freezing system was developed that satisfies the requirements for a repeatable, constant freezing rate. The system was delivered to NIC and is now operational. This report describes the design of the major subsystems, the analyses, the operating procedure, and final system test results.

  7. Advances in Stallion Semen Cryopreservation.

    PubMed

    Alvarenga, Marco Antonio; Papa, Frederico Ozanam; Ramires Neto, Carlos

    2016-12-01

    The use of stallion frozen semen minimizes the spread of disease, eliminates geographic barriers, and preserves the genetic material of the animal for an unlimited time. Significant progress on the frozen thawed stallion semen process and consequently fertility has been achieved over the last decade. These improvements not only increased fertility rates but also allowed cryopreservation of semen from "poor freezers." This article reviews traditional steps and new strategies for stallion semen handling and processing that are performed to overcome the deleterious effects of semen preservation and consequently improve frozen semen quality and fertility. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. MPLM during Expedition 18 / STS-126

    NASA Image and Video Library

    2008-11-19

    S126-E-008120 (18 Nov. 2008) --- Interior view of the Leonardo Multi-Purpose Logistics Module attached to the Earth-facing port of the International Space Station's Harmony node. Leonardo was moved from Space Shuttle Endeavour's cargo bay and linked to the station on Nov. 17, carrying two water recovery systems racks for recycling urine into potable water, a second toilet system, new gallery components, two new food warmers, a food refrigerator, an experiment freezer, combustion science experiment rack, two separate sleeping quarters and a resistance exercise device (aRED) that allows station crewmembers to perform a variety of exercises.

  9. MPLM during Expedition 18 / STS-126

    NASA Image and Video Library

    2008-11-19

    S126-E-008117 (18 Nov. 2008) --- Interior view of the Leonardo Multi-Purpose Logistics Module attached to the Earth-facing port of the International Space Station's Harmony node. Leonardo was moved from Space Shuttle Endeavour's cargo bay and linked to the station on Nov. 17, carrying two water recovery systems racks for recycling urine into potable water, a second toilet system, new gallery components, two new food warmers, a food refrigerator, an experiment freezer, combustion science experiment rack, two separate sleeping quarters and a resistance exercise device (aRED) that allows station crewmembers to perform a variety of exercises.

  10. MPLM during Expedition 18 / STS-126

    NASA Image and Video Library

    2008-11-19

    S126-E-008118 (18 Nov. 2008) --- Interior view of the Leonardo Multi-Purpose Logistics Module attached to the Earth-facing port of the International Space Station's Harmony node. Leonardo was moved from Space Shuttle Endeavour's cargo bay and linked to the station on Nov. 17, carrying two water recovery systems racks for recycling urine into potable water, a second toilet system, new gallery components, two new food warmers, a food refrigerator, an experiment freezer, combustion science experiment rack, two separate sleeping quarters and a resistance exercise device (aRED) that allows station crewmembers to perform a variety of exercises.

  11. STS-40 crewmembers use inflight blood collection system (IBCS) kit on middeck

    NASA Technical Reports Server (NTRS)

    1991-01-01

    STS-40 crewmembers follow procedures for Experiment No. 261, The Influence of Space Flight on Erythrokinetics in Man, while on the middeck of Columbia, Orbiter Vehicle (OV) 102. Payload Specialist F. Drew Gaffney (center) draws blood from Payload Specialist Millie Hughes-Fulford (left) as Mission Specialist (MS) James P. Bagian looks on. The crewmembers are using the inflight blood collection system (IBCS) kit in front of the forward lockers and the orbiter refrigerator freezer (ORF). Displayed on the forward lockers are decals representing the Air Force, the Air Force Reserves (AFRES), University of Tennessee, Colorado State, and Stanford University.

  12. KSC-2009-4294

    NASA Image and Video Library

    2009-07-30

    CAPE CANAVERAL, Fla. – The payload canister rolls onto Launch Pad 39A at NASA's Kennedy Space Center in Florida. Inside is the payload for space shuttle Discovery and the STS-128 mission, the Multi-Purpose Logistics Module Leonardo and the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery's 13-day flight will deliver a new crew member and 33,000 pounds of equipment to the International Space Station. The equipment includes science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch of Discovery on its STS-128 mission is targeted for August 25. Photo credit: NASA/Jack Pfaller.

  13. KSC-2009-4292

    NASA Image and Video Library

    2009-07-30

    CAPE CANAVERAL, Fla. – The payload canister rolls to Launch Pad 39A at NASA's Kennedy Space Center in Florida. Inside is the payload for space shuttle Discovery and the STS-128 mission, the Multi-Purpose Logistics Module Leonardo and the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery's 13-day flight will deliver a new crew member and 33,000 pounds of equipment to the International Space Station. The equipment includes science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch of Discovery on its STS-128 mission is targeted for August 25. Photo credit: NASA/Jack Pfaller.

  14. KSC-2009-4293

    NASA Image and Video Library

    2009-07-30

    CAPE CANAVERAL, Fla. – The payload canister rolls toward Launch Pad 39A at NASA's Kennedy Space Center in Florida. Inside is the payload for space shuttle Discovery and the STS-128 mission, the Multi-Purpose Logistics Module Leonardo and the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery's 13-day flight will deliver a new crew member and 33,000 pounds of equipment to the International Space Station. The equipment includes science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch of Discovery on its STS-128 mission is targeted for August 25. Photo credit: NASA/Jack Pfaller.

  15. Long-term storage of salivary cortisol samples at room temperature

    NASA Technical Reports Server (NTRS)

    Chen, Yu-Ming; Cintron, Nitza M.; Whitson, Peggy A.

    1992-01-01

    Collection of saliva samples for the measurement of cortisol during space flights provides a simple technique for studying changes in adrenal function due microgravity. In the present work, several methods for preserving saliva cortisol at room temperature were investigated using radioimmunoassays for determining cortisol in saliva samples collected on a saliva-collection device called Salivettes. It was found that a pretreatment of Salivettes with citric acid resulted in preserving more than 85 percent of the salivary cortisol for as long as six weeks. The results correlated well with those for a sample stored in a freezer on an untreated Salivette.

  16. [Prevention of adriamycin-induced alopecia by scalp hypothermia with a deep-frozen Duncool-Cap].

    PubMed

    Konishi, Y; Kuroki, T

    1988-11-01

    In order to prevent Adriamycin (ADM)-induced alopecia, scalp hypothermia with a Duncool-Cap frozen in a freezer at -70 degrees C was carried out. Of the 18 patients studied, one patient given total ADM doses of 240 mg developed alopecia of moderate degree, and another patient treated with ADM at a dose level of 50 mg developed mild alopecia. Alopecia could be almost completely prevented in 10 of the 11 patients given total ADM doses of 100 mg or less, and in 6 of the 7 patients given total doses of 200 mg or more.

  17. Development of Methodology and Technology for Identifying and Quantifying Emission Products from Open Burning and Open Detonation Thermal Treatment Methods. Field Test Series A, B, and C. Volume 2, Part A. Quality Assurance and Quality Control

    DTIC Science & Technology

    1992-01-01

    instrument logbook was maintained, but all calibration printouts for the SFC/MS were put in a dedicated loose- leaf notebook. The temperature of the...to-date temperature - monitoring sheets were located at the freezer. Each worker maintained a project-specific personal logbook to enter data...driven 10-cm-diameter gate valve into a 1.5-m3 carbon-impregnated polyethylene ( Velostat 7") sampling bag. The bag, constructed of electrically

  18. Short- and long-term releases of fluorocarbons from disposal of polyurethane foam waste.

    PubMed

    Kjeldsen, Peter; Scheutz, Charlotte

    2003-11-01

    Several halocarbons having very high global warming or ozone depletion potentials have been used as a blowing agent (BA) for insulation foam in home appliances, such as refrigerators and freezers. Many appliances are shredded after the end of their useful life. Release experiments carried out in the laboratory on insulation foam blown with the blowing agents CFC-11, HCFC-141b, HCF-134fa, and HFC-245fa revealed that not all blowing agents are released during a 6-week period following the shredding process. The experiments confirmed the hypothesis that the release could be divided into three segments: By shredding foam panels, a proportion of the closed cells is either split or damaged to a degree allowing for a sudden release of the contained atmosphere in the cell (the instantaneous release). Cells adjacent to the cut surface may be only slightly damaged by tiny cracks or holes allowing a relative slow release of the BA to the surroundings (the short-term release). A significant portion of the cells in the foam particle will be unaffected and only allows release governed by slow diffusion through the PUR cell wall (the long-term release). The magnitude of the releases is for all three types highly dependent on how fine the foam is shredded. The residual blowing agent remaining after the 6-week period may be very slowly released if the integrity of the foam particles with respect to diffusion properties is kept after disposal of the foam waste on landfills. It is shown by setting up a national model simulating the BA releases following decommissioning of used domestic refrigerators/freezers in the United States that the release patterns are highly dependent on how the appliances are shredded.

  19. Effects of high and low temperatures on development time and mortality of house dust mite eggs.

    PubMed

    Mahakittikun, Vanna; Boitano, John Joseph; Ninsanit, Prapakorn; Wangapai, Teerapong; Ralukruedej, Kornraphat

    2011-12-01

    Hatching of Dermatophagoides pteronyssinus eggs was investigated when exposed to temperatures ranging from -70 to +70°C, at varying degrees of humidity (dry and wet heat, direct sunlight) and lengths of time (seven intervals, ranging from 0.5 to 5 h). Exposure to cold was induced using a domestic refrigerator at 4°C, its freezing compartment at -10°C and a deep freezer at -20, -40 and -70°C. For each time interval, there were three slides containing 30 eggs per slide, except for sunlight (five sets of slides). After treatment, all experimental groups were kept on an open work bench for 15 days of observation at ambient room temperature (ca. 22°C) and ca. 75% relative humidity. Control groups with 10 eggs per slide, in triplicate, followed a similar protocol. An egg was considered hatched if an emerging larva was detected from a cracked shell. Results indicated that at 40°C for both dry and wet heat, approximately 80% of all eggs survived. At direct sunlight and dry heat at 50°C, the thermal death point (TDP) occurred at 3 and 5 h, respectively. At 60 and 70°C both wet and dry heat, TDP occurred almost instantaneously. Under cold conditions, only the deep freezer at -70°C was effective in preventing hatching. It may be concluded that exposure to direct sunlight for 3 h, dry/wet heat of 60 and 70°C for a minimum of 30 min, and -70°C prevent egg hatching. This study may have relevance for mite control procedures.

  20. Effects of Agitation and Storage Temperature on Measurements of Hydration Status.

    PubMed

    Adams, Heather M; Eberman, Lindsey E; Yeargin, Susan W; Niemann, Andrew J; Mata, Heather L; Dziedzicki, David J

    2015-12-01

    Hypohydration can have significant implications on normal physiological functions of the body. This study aimed to determine the impact of agitation, storage temperature, and storage time on urine osmolality compared to the criterion control. We used a descriptive diagnostic validity test design. To investigate agitation, we recruited 75 healthy individuals (males = 41, females = 34; mean age = 22 ± 5 years; mean self-reported height = 172 ± 23 cm and mass = 77 ± 17 kg) who provided one or more samples (total = 81). The independent variables were agitation (vortex, hand shaken, no agitation) and temperature (room temperature, freezer, and refrigerator) type. Participants completed informed consent, a health questionnaire and were asked to provide a urine sample, which was split and labeled according to agitation type or storage temperature. Urine osmolality was used to determine hydration status at two time points (within 2 hours [control], 48 hours). We used t-tests to determine the difference between each condition and the control and calculated percent error for each condition. No significant differences for no agitation (t79 = -0.079, P = 0.937), hand shaken (t79 = 1.395, P = 0.167) or vortex mixed (t79 = -0.753, P = 0.453) were identified when compared to the criterion control. No significant differences for room temperature (t82 = -0.720, P = 0.474), refrigerator (t82 = -2.697, P = 0.008) or freezer (t82 = 2.576, P = 0.012) were identified when compared to the criterion control. Our findings suggest agitation of urine specimen is not necessary and samples do not require refrigeration or freezing if assessed within 48 hours. Analysis within two hours of collection is not necessary and samples can be stored for up to 48 hours without impacting the hydration status of the sample.

  1. Seven Years of Permanent Running of MELFI-1 on Board the ISS and Utilisation of the Three MELFI Units Refrigeration Pool

    NASA Technical Reports Server (NTRS)

    Chegancas, Jean; Stephan, Hubertus; Jimenez, Jesus; Campana, Sharon; Hutchison, Susan

    2013-01-01

    The pool of three Minus Eighty Laboratory freezer for ISS (MELFI) units continues providing the scientific community with robust and permanent freezer and refrigeration capabilities for life science experiments on the International Space Station (ISS). Launched in 2006, the first unit will complete, by summer 2013, seven years of continuous operations without intervention on the internal Nitrogen gas cycle, while all necessary hardware and operations were initially planned for preventive maintenance every two years. This unit has demonstrated outstanding performance on orbit and proved the technical decisions made during the development program. Current utilization of MELFI units in the ISS is taking full benefit of the initial specifications, which allows for wide adaptations to cope with the mission scenario imposed by the life extension in orbit. The two other MELFI units, launched respectively in 2008 and 2009, are supporting the first unit providing additional conditioned volume necessary for the science on board, and also for preparing thermal mass used to protect the samples on their way down to earth. The MELFI pool is outfitted with all supporting hardware to allow for extended operation on orbit including preventive and corrective maintenance. The internal components were designed to allow for easy on board maintenance. Spare equipment was installed in the MELFI rack on ISS and specific maintenance means were developed which required crew training before the cold gas cycle could be accessed. The paper will present first how the design choices made for the initial missions are identifying features necessary for extended duration missions, and will then give highlights on the utilization of the MELFI refrigeration pool during the recent years in ISS.

  2. Cold-induced aqueous acetonitrile phase separation: A salt-free way to begin quick, easy, cheap, effective, rugged, safe.

    PubMed

    Shao, Gang; Agar, Jeffrey; Giese, Roger W

    2017-07-14

    Cooling a 1:1 (v/v) solution of acetonitrile and water at -16° C is known to result in two clear phases. We will refer to this event as "cold-induced aqueous acetonitrile phase separation (CIPS)". On a molar basis, acetonitrile is 71.7% and 13.6% in the upper and lower phases, respectively, in our study. The phase separation proceeds as a descending cloud of microdroplets. At the convenient temperature (typical freezer) employed here the lower phase is rather resistant to solidification, although it emerges from the freezer as a solid if various insoluble matter is present at the outset. In a preliminary way, we replaced the initial (salting-out) step of a representative QuEChERS procedure with CIPS, applying this modified procedure ("CIPS-QuEChERS") to a homogenate of salmon (and partly to beef). Three phases resulted, where only the upper, acetonitrile-rich phase is a liquid (that is completely clear). The middle phase comprises ice and precipitated lipids, while the lower phase is the residual matrix of undissolved salmon or meat. Treating the upper phase from salmon, after isolation, with anhydrous MgSO 4 and C18-Si (typical QuEChERS dispersive solid phase extraction sorbents), and injecting into a GC-MS in a nontargeted mode, gives two-fold more preliminary hits for chemicals, and also number of spiked pesticides recovered, relative to that from a comparable QuEChERS method. In part, this is because of much higher background signals in the latter case. Further study of CIPS-QuEChERS is encouraged, including taking advantage of other QuERChERS conditions. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Effects of drying, packaging, and temperature on the quality of fried onion slices.

    PubMed

    Asefi, Narmela; Mozaffari, Mansoure

    2010-06-01

    Onion is used widely as a condiment for meat and other types of food in Iran. Because of the high sensitivity of this product to storage conditions and having not enough storage under controlled atmosphere (CA), the effects of drying method, packaging, and temperature during storage on thin slices of fried red Azershahr variety onion were investigated. To prepare this processed food with excellent organoleptic properties and useful in the food service industry onions were sliced (2 mm), deep-fried with corn frying oil in a batch fryer at 150 degrees C, and dried to a water content of 3-4% in a hot-air dryer at 70 degrees C for 24 h and a microwave system. Samples were packed in aluminum foil, which was purged by nitrogen gas, and cellophane-sealed with thermal sewing. Then these samples were kept for one year at laboratory temperature and in a freezer at -18 degrees C. During the storage time the moisture content, peroxide value, vitamin C content, total microbial count (TMC), and organoleptic characteristics of the samples were analyzed every 2 mo. The results of microbial analyses indicated that after 6 mo all of the samples had higher TMCs than the permission limits so the expiration date was set before that. The moisture contents and peroxide values showed a noticeable increase, probably due to seal failure, whereas vitamin C decreased up to 66% during storage. The loss of sensory properties was parallel to the microbial and chemical results. The findings revealed that the best sample was oven-dried, packed in aluminum foil under inert gas, and kept in a freezer (OAF) up to 6 mo.

  4. Effects of freezer storage time on levels of complement biomarkers.

    PubMed

    Morgan, Angharad R; O'Hagan, Caroline; Touchard, Samuel; Lovestone, Simon; Morgan, B Paul

    2017-11-06

    There is uncertainty regarding how stable complement analytes are during long-term storage at - 80 °C. As part of our work program we have measured 17 complement biomarkers (C1q, C1 inhibitor, C3, C3a, iC3b, C4, C5, C9, FB, FD, FH, FI, TCC, Bb, sCR1, sCR2, Clusterin) and the benchmark inflammatory marker C-reactive protein (CRP) in a large set of plasma samples (n = 720) that had been collected, processed and subsequently stored at - 80 °C over a period of 6.6-10.6 years, prior to laboratory analysis. The biomarkers were measured using solid-phase enzyme immunoassays with a combination of multiplex assays using the MesoScale Discovery Platform and single-plex enzyme-linked immunosorbent assays (ELISAs). As part of a post hoc analysis of extrinsic factors (co-variables) affecting the analyses we investigated the impact of freezer storage time on the values obtained for each complement analyte. With the exception of five analytes (C4, C9, sCR2, clusterin and CRP), storage time was significantly correlated with measured plasma concentrations. For ten analytes: C3, FI, FB, FD, C5, sCR1, C3a, iC3b, Bb and TCC, storage time was positively correlated with concentration and for three analytes: FH, C1q, and C1 inhibitor, storage time was negatively correlated with concentration. The results suggest that information on storage time should be regarded as an important co-variable and taken into consideration when analysing data to look for associations of complement biomarker levels and disease or other outcomes.

  5. Alternative refrigerants and refrigeration cycles for domestic refrigerators

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sand, J.R.; Rice, C.L.; Vineyard, E.A.

    1992-12-01

    This project initially focused on using nonazeotropic refrigerant mixtures (NARMs) in a two-evaporator refrigerator-freezer design using two stages of liquid refrigerant subcooling. This concept was proposed and tested in 1975. The work suggested that the concept was 20% more efficient than the conventional one-evaporator refrigerator-freezer (RF) design. After considerable planning and system modeling based on using a NARM in a Lorenz-Meutzner (L-M) RF, the program scope was broadened to include investigation of a ``dual-loop`` concept where energy savings result from exploiting the less stringent operating conditions needed to satisfy cooling, of the fresh food section. A steady-state computer model (CYCLE-Z)more » capable of simulating conventional, dual loop, and L-M refrigeration cycles was developed. This model was used to rank the performance of 20 ozone-safe NARMs in the L-M refrigeration cycle while key system parameters were systematically varied. The results indicated that the steady-state efficiency of the L-M design was up to 25% greater than that of a conventional cycle. This model was also used to calculate the performance of other pure refrigerants relative to that of dichlorodifluoromethane, R-12, in conventional and dual-loop RF designs. Projected efficiency gains for these cycles were more modest, ranging from 0 to 10%. Individual compressor calorimeter tests of nine combinations of evaporator and condenser temperatures usually used to map RF compressor performance were carried out with R-12 and two candidate L-M NARMs in several compressors. Several models of a commercially produced two-evaporator RF were obtained as test units. Two dual-loop RF designs were built and tested as part of this project.« less

  6. Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects

    PubMed Central

    Kriebardis, Anastasios G.; Antonelou, Marianna H.; Georgatzakou, Hara T.; Tzounakas, Vassilis L.; Stamoulis, Konstantinos E.; Papassideri, Issidora S.

    2016-01-01

    Background Extracellular vesicles or microparticles exhibiting procoagulant and thrombogenic activity may contribute to the haemostatic potential of fresh frozen plasma. Materials and methods Fresh frozen plasma was prepared from platelet-rich plasma at 20 °C (Group-1 donors) or directly from whole blood at 4 °C (Group-2 donors). Each unit was aseptically divided into three parts, stored frozen for specific periods of time, and analysed by flow cytometry for procoagulant activity immediately after thaw or following post-thaw storage for 24 h at 4 °C. Donors’ haematologic, biochemical and life-style profiles as well as circulating microparticles were analysed in parallel. Results Circulating microparticles exhibited a considerable interdonor but not intergroup variation. Fresh frozen plasma units were enriched in microparticles compared to plasma in vivo. Duration of storage significantly affected platelet- and red cell-derived microparticles. Fresh frozen plasma prepared directly from whole blood contained more residual platelets and more platelet-derived microparticles compared to fresh frozen plasma prepared from platelet-rich plasma. Consequently, there was a statistically significant difference in total, platelet- and red cell-derived microparticles between the two preparation protocols over storage time in the freezer. Preservation of the thawed units for 24 h at 4 °C did not significantly alter microparticle accumulation. Microparticle accumulation and anti-oxidant capacity of fresh frozen plasma was positively or negatively correlated, respectively, with the level of circulating microparticles in individual donors. Discussion The preparation protocol and the duration of storage in the freezer, independently and in combination, influenced the accumulation of microparticles in fresh frozen plasma units. In contrast, storage of thawed units for 24 h at 4 °C had no significant effect on the concentration of microparticles. PMID:27136430

  7. Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects.

    PubMed

    Kriebardis, Anastasios G; Antonelou, Marianna H; Georgatzakou, Hara T; Tzounakas, Vassilis L; Stamoulis, Konstantinos E; Papassideri, Issidora S

    2016-05-01

    Extracellular vesicles or microparticles exhibiting procoagulant and thrombogenic activity may contribute to the haemostatic potential of fresh frozen plasma. Fresh frozen plasma was prepared from platelet-rich plasma at 20 °C (Group-1 donors) or directly from whole blood at 4 °C (Group-2 donors). Each unit was aseptically divided into three parts, stored frozen for specific periods of time, and analysed by flow cytometry for procoagulant activity immediately after thaw or following post-thaw storage for 24 h at 4 °C. Donors' haematologic, biochemical and life-style profiles as well as circulating microparticles were analysed in parallel. Circulating microparticles exhibited a considerable interdonor but not intergroup variation. Fresh frozen plasma units were enriched in microparticles compared to plasma in vivo. Duration of storage significantly affected platelet- and red cell-derived microparticles. Fresh frozen plasma prepared directly from whole blood contained more residual platelets and more platelet-derived microparticles compared to fresh frozen plasma prepared from platelet-rich plasma. Consequently, there was a statistically significant difference in total, platelet- and red cell-derived microparticles between the two preparation protocols over storage time in the freezer. Preservation of the thawed units for 24 h at 4 °C did not significantly alter microparticle accumulation. Microparticle accumulation and anti-oxidant capacity of fresh frozen plasma was positively or negatively correlated, respectively, with the level of circulating microparticles in individual donors. The preparation protocol and the duration of storage in the freezer, independently and in combination, influenced the accumulation of microparticles in fresh frozen plasma units. In contrast, storage of thawed units for 24 h at 4 °C had no significant effect on the concentration of microparticles.

  8. Hydrophilic structure for condensation management on the movable mullion of a refrigerator

    DOEpatents

    Kuehl, Steven John; Vonderhaar, John J; Wu, Guolian; Wu, Mianxue

    2015-01-06

    A refrigerator appliance that includes a freezer compartment, a refrigeration compartment having two doors, and a mullion having an interior surface and an exterior surface. The mullion is movably coupled to, and configured to swing behind, at least one of the doors when the two doors are moved to a closed position. The exterior surface of the mullion directs condensation toward a transfer point. The exterior surface may be configured with a hydrophilic surface to direct and control the condensation. The condensation may also be directed into a receptacle or a wicking structure arranged to facilitate evaporation of the unwanted condensate.

  9. STS-40 crewmembers use inflight blood collection system (IBCS) kit on middeck

    NASA Technical Reports Server (NTRS)

    1991-01-01

    STS-40 crewmembers follow procedures for Experiment No. 261, The Influence of Space Flight on Erythrokinetics in Man, while on the middeck of Columbia, Orbiter Vehicle (OV) 102. Mission Specialist (MS) James P. Bagian (right) draws blood from Payload Specialist F. Drew Gaffney (center) as the second Spacelab Life Sciences 1 (SLS-1) Payload Specialist Millie Hughes-Fulford looks on. The crewmembers are using the inflight blood collection system (IBCS) kit in front of the forward lockers and the orbiter refrigerator freezer (ORF). Displayed on the forward lockers are decals representing the University of Tennessee, Colorado State, and Stanford University and several drink containers.

  10. Rewards of renewables

    NASA Astrophysics Data System (ADS)

    McNamee, Gregory

    2008-09-01

    In 1987 an American-style fridge freezer would use about 950 kWh of electricity and cost about 150 (£80) a year to run. Two decades on, a comparable appliance uses half the electricity and costs less than half as much to run. In 1975 there were about 3780 000 cars on the streets of Los Angeles, whereas today there are more than 5200 000 - yet air-pollution levels have fallen by half and an increasing number of those vehicles are hybrids or rely on renewable fuels like bio-diesel. Last year, half a million homes in Southern California were receiving direct solar power, either from solar electricity plants or from rooftop photovoltaic panels.

  11. Note: A microfluidic freezer based on evaporative cooling of atomized aqueous microdroplets

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Song, Jin; Kim, Dohyun, E-mail: dohyun.kim@mju.ac.kr; Chung, Minsub

    2015-01-15

    We report for the first time water-based evaporative cooling integrated into a microfluidic chip for temperature control and freezing of biological solution. We opt for water as a nontoxic, effective refrigerant. Aqueous solutions are atomized in our device and evaporation of microdroplets under vacuum removes heat effectively. We achieve rapid cooling (−5.1 °C/s) and a low freezing temperature (−14.1 °C). Using this approach, we demonstrate freezing of deionized water and protein solution. Our simple, yet effective cooling device may improve many microfluidic applications currently relying on external power-hungry instruments for cooling and freezing.

  12. A whole-plant screening test to identify genotypes with superior freezing tolerance.

    PubMed

    Bertrand, Annick; Castonguay, Yves; Bourassa, Josée

    2014-01-01

    Freezing tolerance is a determinant factor of persistence of perennials grown in northern climate. Selection for winterhardiness in field nurseries is difficult because of the unpredictability of occurrence of test winters allowing the identification of hardy genotypes. Here we describe a whole-plant assay entirely performed indoor in growth chambers and walk-in freezers to identify genotypes with superior tolerance to freezing within populations of open pollinated species. Three successive freezing stresses are applied to progressively eliminate 90 % of the population and to retain only the 10 % best performing genotypes. This approach can be used to generate recurrently selected populations more tolerant to freezing in different species.

  13. [In vitro activity of human bone marrow cells after cryopreservation in liquid nitrogen for 21 - 25 years].

    PubMed

    Huang, You-Zhang; Shen, Jian-Liang; Gong, Li-Zhong; Zheng, Pei-Hao; Liu, Yi; Yin, Wen-Jie; Cen, Jian; Wang, Ning; Zhao, De-Feng

    2010-02-01

    The aim of this study was to investigate the best method to preserve human bone marrow cells and the effectiveness of long term cryopreservation at -80 degrees C. The human bone marrow cells in 20 samples were firstly frozen by a programmed freezer or -80 degrees C refrigerator, and then were preserved in liquid nitrogen with DMSO-AuP (10% dimethylsulfonamide, 10% autologous plasma) or DMSO-HES-HuA (5% dimethylsulfonamide, 6% hydroxyethyl starch, 4% human serum albumin) as cryoprotectant for 21 to 25 years. They were thawed in 38 degrees C. The cell sample frozen in -80 degrees C refrigerator was frozen at a low frozen speed of 1 degrees C/min which was the same as the programmed freezer before -30 degrees C. Before detection the bone marrow cells were taken from liquid nitrogen and were thawed in 38 degrees C, then the suspension of bone marrow cells was prepared for detection. The cell morphology and recovery rate of erythrocytes, nucleocytes and platelets; the recovery rate of hematopoietic stem progenitors cells, as well as mesenchymal stem cells were determined. The results showed that the protective effectiveness of DMSO-HES-HuA was better than DMSO-AuP. The mature erythrocytes were destroyed lightly [(3.5 +/- 1.5)% versus (12.6 +/- 4.8)%], the hemolysis rate was lower [(3.3 +/- 1.6)% versus (23.1 +/- 5.1)%]. Osmotic fragility of erythrocytes in the former was not changed, but was dropped in the latter. The recovery rates of red cell, platelet, granulocyte-macrophage colony forming units and long term culture-initiating cells were higher in the former than that in the latter [(96.1 +/- 1.8)%, (70.0 +/- 9.5)%, (49.2 +/- 10.9)%, (54.2 +/- 13.8)% versus (76.3 +/- 5.6)%, (52.7 +/- 8.1)%, (43.5 +/- 12.3)%, (47.2 +/- 13.6)% respectively]. With each kind of cryoprotectant or frozen method, the frozen MSC could keep the original growth properties. With the same cryoprotectant and different frozen method, the cryopreservative effectiveness was not different. The influence of the cryoprotectant prescriptions and the frozen methods on the cryopreservative effectiveness was little. It is concluded that the human bone marrow cells with DMSO-AuP or DMSO-HES-HuA as cryoprotectant, frozen by a programmed freezer or -80 degrees C refrigerator, could be then preserved in liquid nitrogen for long time. When the preserving time was as long as 21 to 25 years, the morphology, the recovery rate and the activity of various kinds of cells were still good. The method of freezing by -80 degrees C refrigerator with 5% DMSO-6% HES-4% HuA and preserving in liquid nitrogen would be convenient, cheap and easily-manipulated for preservation of the human bone marrow cells.

  14. NASA Snaps Picture of Eastern U.S. in a Record-Breaking "Freezer"

    NASA Image and Video Library

    2015-02-20

    NASA's Terra satellite captured an image of the snow-covered eastern U.S. that looks like the states have been sitting in a freezer. In addition to the snow cover, Arctic and Siberian air masses have settled in over the Eastern U.S. triggering many record low temperatures in many states. On Feb. 19 at 16:40 UTC (11:40 a.m. EST), the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument that flies aboard NASA's Terra satellite captured a picture of the snowy landscape. The snow cover combined with the frosty air mass made the eastern U.S. feel like the inside of freezer. The MODIS image was created at NASA's Goddard Space Flight Center in Greenbelt, Maryland. On the morning of Feb. 20, NOAA's Weather Prediction Center (WPC) noted, "There were widespread subzero overnight lows Thursday night (Feb. 19) extending from Illinois to western Virginia, and numerous record lows were set. Bitterly-cold arctic air is setting numerous temperature records across the eastern U.S. and will keep temperatures well below normal on Friday (Feb. 20)." In Baltimore, Maryland, a low temperature of 1F broke the record low for coldest morning recorded at the Thurgood Marshall Baltimore Washington-International Airport. In Louisville, Kentucky, temperatures dropped to -6F, breaking the old record low of 0F, according to meteorologist Brian Goode of WAVE-TV. Meanwhile, Richmond Kentucky bottomed out at a frigid -32F. In North Carolina, a record low temperature was set at Charlotte where the overnight temperature bottomed out at 7F breaking the old record of 13F in 1896. In Asheville, temperatures dropped to just 4F breaking the old record of 10F in 1979. Temperature records for Asheville extend back to 1876. Several records were also broken in Georgia, according to Matt Daniel, a meteorologist at WMAZ-TV, Macon Georgia, who cited data from the National Weather Service. Daniel said that Macon set a new record low when the temperature dropped to 18F, beating the previous record of 21F set in 1958. Athens broke a new record low, too dropping to 14F and beating the old record of 18F set in 1958/1928. NOAA's NPC noted that "Highs on Friday (Feb. 20) will struggle to get out of the teens from the Ohio Valley to the Mid-Atlantic region. After Friday, temperatures are forecast to moderate and get closer to February averages as a storm system approaches from the west." Image Credit: NASA Goddard MODIS Rapid Response Team NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

  15. Parameters estimation of sandwich beam model with rigid polyurethane foam core

    NASA Astrophysics Data System (ADS)

    Barbieri, Nilson; Barbieri, Renato; Winikes, Luiz Carlos

    2010-02-01

    In this work, the physical parameters of sandwich beams made with the association of hot-rolled steel, Polyurethane rigid foam and High Impact Polystyrene, used for the assembly of household refrigerators and food freezers are estimated using measured and numeric frequency response functions (FRFs). The mathematical models are obtained using the finite element method (FEM) and the Timoshenko beam theory. The physical parameters are estimated using the amplitude correlation coefficient and genetic algorithm (GA). The experimental data are obtained using the impact hammer and four accelerometers displaced along the sample (cantilevered beam). The parameters estimated are Young's modulus and the loss factor of the Polyurethane rigid foam and the High Impact Polystyrene.

  16. Learning by Erring: fire!

    PubMed

    Bjugn, Roger; Hansen, Jarle

    2013-08-01

    Biorepositories may be affected by a number of emergencies ranging from bad publicity to natural disasters, and biorepositories should have plans for handling such situations. The emergency management process includes all phases from mitigation to recovery. Fire is one disaster that may cause extensive damage to both physical structures and humans. In this article, we analyze events related to a fire in a storage facility for mechanical freezers. The analysis covers both the pre-crisis stage, the fire itself, and the post-crisis stage. Even the best intended planning cannot stop a crisis from happening. However, an open-minded analysis of the crisis with focus on learning and quality improvement can improve an organization's ability to handle the next emergency situation.

  17. KSC-2009-4535

    NASA Image and Video Library

    2009-08-07

    CAPE CANAVERAL, Fla. – STS-128 Mission Specialist Patrick Forrester is the White Room on NASA Kennedy Space Center's Launch Pad 39A getting ready to enter space shuttle Discovery. The White Room is at the end of the orbiter access arm and provides entry into the shuttle. Mission crew members are at Kennedy to take part in the terminal countdown demonstration test, or TCDT, which culminates in a simulated launch countdown inside the shuttle. On the STS-128 mission, Discovery will deliver 33,000 pounds of equipment to the station, including science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch is targeted for late August. Photo credit: NASA/Jim Grossmann

  18. KSC-2009-4533

    NASA Image and Video Library

    2009-08-07

    CAPE CANAVERAL, Fla. – STS-128 Pilot Kevin Ford is the White Room on NASA Kennedy Space Center's Launch Pad 39A getting ready to enter space shuttle Discovery. The White Room is at the end of the orbiter access arm and provides entry into the shuttle. Mission crew members are at Kennedy to take part in the terminal countdown demonstration test, or TCDT, which culminates in a simulated launch countdown inside the shuttle. On the STS-128 mission, Discovery will deliver 33,000 pounds of equipment to the station, including science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch is targeted for late August. Photo credit: NASA/Jim Grossmann

  19. KSC-2009-4538

    NASA Image and Video Library

    2009-08-07

    CAPE CANAVERAL, Fla. – In the White Room on NASA Kennedy Space Center's Launch Pad 39A, STS-128 Commander Rick Sturckow is helped with his harness before entering space shuttle Discovery. The White Room is at the end of the orbiter access arm and provides entry into the shuttle. The crew is at Kennedy to take part in the terminal countdown demonstration test, or TCDT, which includes equipment familiarization, emergency exit training and a simulated countdown. On the STS-128 mission, Discovery will deliver 33,000 pounds of equipment to the station, including science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch is targeted for late August. Photo credit: NASA/Kim Shiflett

  20. Comparison of Traditional and Innovative Techniques to Solve Technical Challenges

    NASA Technical Reports Server (NTRS)

    Perchonok, Michele

    2011-01-01

    This slide presentation reviews the use of traditional and innovative techniques to solve technical challenges in food storage technology. The planning for a mission to Mars is underway, and the food storage technology improvements requires that improvements be made. This new technology is required, because current food storage technology is inadequate,refrigerators or freezers are not available for food preservation, and that a shelf life of 5 years is expected. A 10 year effort to improve food packaging technology has not enhanced significantly food packaging capabilities. Two innovation techniques were attempted InnoCentive and Yet2.com and have provided good results, and are still under due diligence for solver verification.

  1. Lithography of Polymer Nanostructures on Glass for Teaching Polymer Chemistry and Physics.

    PubMed

    Sahar-Halbany, Adi; Vance, Jennifer M; Drain, Charles Michael

    2011-05-01

    As nanolithography becomes increasingly important in technology and daily life, a variety of inexpensive and creative methods toward communicating the concepts underpinning these processes in the classroom are necessary. An experiment is described that uses simple CD-Rs, C-clamps, an oven, and a freezer to provide concrete examples and insights into the chemistry and principles of nanolithography. The experiment also has flexibility, making it suitable for a range of classroom levels from high school to more advanced labs in college. Because CD-Rs are composed of grooves of polycarbonate, the experiment provides a basis for discussions and exploration into the chemistry and physics of polymers on the nanoscale.

  2. Cold Stowage: An ISS Project

    NASA Technical Reports Server (NTRS)

    Hartley, Garen

    2018-01-01

    NASA's vision for humans pursuing deep space flight involves the collection of science in low earth orbit aboard the International Space Station (ISS). As a service to the science community, Johnson Space Center (JSC) has developed hardware and processes to preserve collected science on the ISS and transfer it safely back to the Principal Investigators. This hardware includes an array of freezers, refrigerators, and incubators. The Cold Stowage team is part of the International Space Station (ISS) program. JSC manages the operation, support and integration tasks provided by Jacobs Technology and the University of Alabama Birmingham (UAB). Cold Stowage provides controlled environments to meet temperature requirements during ascent, on-orbit operations and return, in relation to International Space Station Payload Science.

  3. Development of isotope labeling LC-MS for human salivary metabolomics and application to profiling metabolome changes associated with mild cognitive impairment.

    PubMed

    Zheng, Jiamin; Dixon, Roger A; Li, Liang

    2012-12-18

    Saliva is a readily available biofluid that may contain metabolites of interest for diagnosis and prognosis of diseases. In this work, a differential (13)C/(12)C isotope dansylation labeling method, combined with liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR-MS), is described for quantitative profiling of the human salivary metabolome. New strategies are presented to optimize the sample preparation and LC-MS detection processes. The strategies allow the use of as little of 5 μL of saliva sample as a starting material to determine the concentration changes of an average of 1058 ion pairs or putative metabolites in comparative saliva samples. The overall workflow consists of several steps including acetone-induced protein precipitation, (12)C-dansylation labeling of the metabolites, and LC-UV measurement of the total concentration of the labeled metabolites in individual saliva samples. A pooled sample was prepared from all the individual samples and labeled with (13)C-dansylation to serve as a reference. Using this metabolome profiling method, it was found that compatible metabolome results could be obtained after saliva samples were stored in tubes normally used for genetic material collection at room temperature, -20 °C freezer, and -80 °C freezer over a period of 1 month, suggesting that many saliva samples already collected in genomic studies could become a valuable resource for metabolomics studies, although the effect of much longer term of storage remains to be determined. Finally, the developed method was applied for analyzing the metabolome changes of two different groups: normal healthy older adults and comparable older adults with mild cognitive impairment (MCI). Top-ranked 18 metabolites successfully distinguished the two groups, among which seven metabolites were putatively identified while one metabolite, taurine, was definitively identified.

  4. Photochemical syntheses, transformations, and bioorthogonal chemistry of trans-cycloheptene and sila trans-cycloheptene Ag(i) complexes† †Electronic supplementary information (ESI) available: Full procedures, computational details and characterization data. CCDC 1583975 and 1583976. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7sc04773h

    PubMed Central

    Fang, Yinzhi; Zhang, Han; Huang, Zhen; Scinto, Samuel L.; Yang, Jeffrey C.; am Ende, Christopher W.; Dmitrenko, Olga; Johnson, Douglas S.

    2018-01-01

    A photochemical synthesis of AgNO3 complexes of trans-cycloheptene (TCH) and trans-1-sila-4-cycloheptene (Si-TCH) derivatives is described. A low temperature flow photoreactor was designed to enable the synthesis of carbocyclic TCH derivatives due to their thermal sensitivity in the absence of metal coordination. Unlike the free carbocycles, TCH·AgNO3 complexes can be handled at rt and stored for weeks in the freezer (–18 °C). Si-TCH·AgNO3 complexes are especially robust, and are bench stable for days at rt, and for months in the freezer. X-ray crystallography was used to characterize a Si-TCH·AgNO3 complex for the first time. With decomplexation of AgNO3in situ, metal-free TCO and Si-TCH derivatives can engage in a range of cycloaddition reactions as well as dihydroxylation reactions. Computation was used to predict that Si-TCH would engage in bioorthogonal reactions that are more rapid than the most reactive trans-cyclooctenes. Metal-free Si-TCH derivatives were shown to display good stability in solution, and to engage in the fastest bioorthogonal reaction reported to date (k2 1.14 × 107 M–1 s–1 in 9 : 1 H2O : MeOH). Utility in bioorthogonal protein labeling in live cells is described, including labeling of GFP with an unnatural tetrazine-containing amino acid. The reactivity and specificity of the Si-TCH reagents with tetrazines in live mammalian cells was also evaluated using the HaloTag platform. The cell labeling experiments show that Si-TCH derivatives are best suited as probe molecules in the cellular environment. PMID:29675242

  5. Utility of a Work Process Classification System for characterizing non-fatal injuries in the Alaskan commercial fishing industry

    PubMed Central

    Syron, Laura N.; Lucas, Devin L.; Bovbjerg, Viktor E.; Bethel, Jeffrey W.; Kincl, Laurel D.

    2016-01-01

    Background The US commercial fishing industry is hazardous, as measured by mortality data. However, research on non-fatal injuries is limited. Non-fatal injuries constitute the majority of occupational injuries and can result in workers’ lowered productivity and wages, lost quality of life, and disability. In the United States, a Work Process Classification System (WPCS) has previously been applied in Alaskan freezer-trawl and freezer-longline fleets to identify causes of injuries and specific hazards, but not to other fishing fleets. Objectives This descriptive epidemiologic study aimed to explore the application and modification of the WPCS in multiple Alaskan fleets, characterize non-fatal occupational injuries in these fleets, and identify work processes that could be targeted for further investigation and future injury prevention efforts. Design Traumatic, non-fatal injuries on-board Alaskan commercial fishing vessels were identified through United States Coast Guard investigative reports. Characteristics of injuries, as well as worker characteristics, were analysed. Injuries were coded using the WPCS. Results We successfully utilized the WPCS to code non-fatal injury cases (n = 136). The most frequent main work processes associated with non-fatal injuries included: on-board trawlers, handling frozen fish and processing the catch; on-board vessels using pot/trap gear, handling the gear and shooting/setting the gear; on-board longliners, traffic on board and hauling the gear; and on-board processor vessels, processing the catch, other work with the catch, and handling frozen fish. Conclusions The study confirmed that a WPCS can be applied to multiple Alaskan fleets to identify hazardous tasks. Hazards were unique for each vessel gear type. Future injury prevention efforts should target work processes associated with the most frequent and most severe injuries. Future studies should establish time estimates for work processes in order to determine risk estimates. Efforts to improve non-fatal injury reporting, especially on smaller commercial fishing vessels, should be undertaken. PMID:26782030

  6. Cranioplasty: Indications, procedures, and outcome - An institutional experience.

    PubMed

    Andrabi, Syed M; Sarmast, Arif H; Kirmani, Altaf R; Bhat, Abdul R

    2017-01-01

    Cranioplasty, the repair of a skull vault defect by insertion of an object (bone or nonbiological materials such as metal or plastic plates), is a well-known procedure in modern neurosurgery. Brain protection and cosmetic aspects are the major indications of cranioplasty. A retroprospective study was conducted for evaluating the indications, materials used, complications, and outcome of cranioplasty. This study was prospective from August 2013 to September 2015 and retrospective from August 2010 to July 2013. In the retrospective study, patients files were retrieved from the mentioned date (August 2010 to July 2013) from the medical records and the findings were recorded. Abstracted data included age at the time of cranioplasty (years), sex (male or female), medical comorbidities (hypertension, diabetes), indications for craniectomy [Road traffic accident (RTA), fall from height (FFH), hit by stone or cricket ball, physical assault, stroke, infection, shell injury, bullet injury, and intraoperative swelling], laterality of cranioplasty (bilateral, unilateral, or bifrontal), time between craniectomy and cranioplasty (weeks), type of graft (autologous or artificial), type of prosthesis if used (methylmethacrylate, titanium), storage of bone flap if used (subcutaneous or deep freezer), operative time (minutes), and complications fallowing cranioplasty. Of the 236 patients included in the study, maximum were in the age group of 21-30 years i.e., 30.93% ( n = 73). Mean age of the patients was 33.44 years. A total of 196 (83.05%) were autologous and 40 (16.95%) were artificial. Out of the 40 patients who underwent artificial cranioplasty, 36 (15.25%) had methylmethacrylate graft and 4 (1.7%) had titanium mesh implant. Bone was not preserved in 16.95% ( n = 40), preserved in subcutaneous tissue in abdominal wall in 2.54% ( n = 6), and preserved in deep freezer in 80.51% ( n = 190) of the patients. Cranioplasty as a procedure is not without complications; however, if performed properly and at proper time with an aseptic technique, good results are achieved.

  7. Utility of a Work Process Classification System for characterizing non-fatal injuries in the Alaskan commercial fishing industry.

    PubMed

    Syron, Laura N; Lucas, Devin L; Bovbjerg, Viktor E; Bethel, Jeffrey W; Kincl, Laurel D

    2016-01-01

    Background The US commercial fishing industry is hazardous, as measured by mortality data. However, research on non-fatal injuries is limited. Non-fatal injuries constitute the majority of occupational injuries and can result in workers' lowered productivity and wages, lost quality of life, and disability. In the United States, a Work Process Classification System (WPCS) has previously been applied in Alaskan freezer-trawl and freezer-longline fleets to identify causes of injuries and specific hazards, but not to other fishing fleets. Objectives This descriptive epidemiologic study aimed to explore the application and modification of the WPCS in multiple Alaskan fleets, characterize non-fatal occupational injuries in these fleets, and identify work processes that could be targeted for further investigation and future injury prevention efforts. Design Traumatic, non-fatal injuries on-board Alaskan commercial fishing vessels were identified through United States Coast Guard investigative reports. Characteristics of injuries, as well as worker characteristics, were analysed. Injuries were coded using the WPCS. Results We successfully utilized the WPCS to code non-fatal injury cases (n = 136). The most frequent main work processes associated with non-fatal injuries included: on-board trawlers, handling frozen fish and processing the catch; on-board vessels using pot/trap gear, handling the gear and shooting/setting the gear; on-board longliners, traffic on board and hauling the gear; and on-board processor vessels, processing the catch, other work with the catch, and handling frozen fish. Conclusions The study confirmed that a WPCS can be applied to multiple Alaskan fleets to identify hazardous tasks. Hazards were unique for each vessel gear type. Future injury prevention efforts should target work processes associated with the most frequent and most severe injuries. Future studies should establish time estimates for work processes in order to determine risk estimates. Efforts to improve non-fatal injury reporting, especially on smaller commercial fishing vessels, should be undertaken.

  8. Utility of a Work Process Classification System for characterizing non-fatal injuries in the Alaskan commercial fishing industry.

    PubMed

    Syron, Laura N; Lucas, Devin L; Bovbjerg, Viktor E; Bethel, Jeffrey W; Kincl, Laurel D

    2016-01-01

    The US commercial fishing industry is hazardous, as measured by mortality data. However, research on non-fatal injuries is limited. Non-fatal injuries constitute the majority of occupational injuries and can result in workers' lowered productivity and wages, lost quality of life, and disability. In the United States, a Work Process Classification System (WPCS) has previously been applied in Alaskan freezer-trawl and freezer-longline fleets to identify causes of injuries and specific hazards, but not to other fishing fleets. This descriptive epidemiologic study aimed to explore the application and modification of the WPCS in multiple Alaskan fleets, characterize non-fatal occupational injuries in these fleets, and identify work processes that could be targeted for further investigation and future injury prevention efforts. Traumatic, non-fatal injuries on-board Alaskan commercial fishing vessels were identified through United States Coast Guard investigative reports. Characteristics of injuries, as well as worker characteristics, were analysed. Injuries were coded using the WPCS. We successfully utilized the WPCS to code non-fatal injury cases (n = 136). The most frequent main work processes associated with non-fatal injuries included: on-board trawlers, handling frozen fish and processing the catch; on-board vessels using pot/trap gear, handling the gear and shooting/setting the gear; on-board longliners, traffic on board and hauling the gear; and on-board processor vessels, processing the catch, other work with the catch, and handling frozen fish. The study confirmed that a WPCS can be applied to multiple Alaskan fleets to identify hazardous tasks. Hazards were unique for each vessel gear type. Future injury prevention efforts should target work processes associated with the most frequent and most severe injuries. Future studies should establish time estimates for work processes in order to determine risk estimates. Efforts to improve non-fatal injury reporting, especially on smaller commercial fishing vessels, should be undertaken.

  9. Buffer Gas Acquisition and Storage

    NASA Technical Reports Server (NTRS)

    Parrish, Clyde F.; Lueck, Dale E.; Jennings, Paul A.; Callahan, Richard A.; Delgado, H. (Technical Monitor)

    2001-01-01

    The acquisition and storage of buffer gases (primarily argon and nitrogen) from the Mars atmosphere provides a valuable resource for blanketing and pressurizing fuel tanks and as a buffer gas for breathing air for manned missions. During the acquisition of carbon dioxide (CO2), whether by sorption bed or cryo-freezer, the accompanying buffer gases build up in the carbon dioxide acquisition system, reduce the flow of CO2 to the bed, and lower system efficiency. It is this build up of buffer gases that provide a convenient source, which must be removed, for efficient capture Of CO2 Removal of this buffer gas barrier greatly improves the charging rate of the CO2 acquisition bed and, thereby, maintains the fuel production rates required for a successful mission. Consequently, the acquisition, purification, and storage of these buffer gases are important goals of ISRU plans. Purity of the buffer gases is a concern e.g., if the CO, freezer operates at 140 K, the composition of the inert gas would be approximately 21 percent CO2, 50 percent nitrogen, and 29 percent argon. Although there are several approaches that could be used, this effort focused on a hollow-fiber membrane (HFM) separation method. This study measured the permeation rates of CO2, nitrogen (ND, and argon (Ar) through a multiple-membrane system and the individual membranes from room temperature to 193K and 10 kpa to 300 kPa. Concentrations were measured with a gas chromatograph that used a thermoconductivity (TCD) detector with helium (He) as the carrier gas. The general trend as the temperature was lowered was for the membranes to become more selective, In addition, the relative permeation rates between the three gases changed with temperature. The end result was to provide design parameters that could be used to separate CO2 from N2 and Ar.

  10. Objective assessment of skin tightening in Asians using a water-filtered near-infrared (1,000–1,800 nm) device with contact-cooling and freezer-stored gel

    PubMed Central

    Tanaka, Yohei; Tsunemi, Yuichiro; Kawashima, Makoto; Tatewaki, Naoto; Nishida, Hiroshi

    2013-01-01

    Background Near-infrared has been shown to penetrate deeper than optical light sources independent of skin color, allowing safer treatment for the Asian skin type. Many studies have indicated the efficacy of various types of devices, but have not included a sufficiently objective evaluation. In this study, we used three-dimensional imaging for objective evaluation of facial skin tightening using a water-filtered near-infrared device. Methods Twenty Japanese patients were treated with the water-filtered near-infrared (1,000–1,800 nm) device using a contact-cooling and nonfreezing gel stored in a freezer. Three-dimensional imaging was performed, and quantitative volume measurements were taken to evaluate the change in post-treatment volume. The patients then provided their subjective assessments. Results Objective assessments of the treated cheek volume evaluated by a three-dimensional color schematic representation with quantitative volume measurements showed significant improvement 3 months after treatment. The mean volume reduction at the last post-treatment visit was 2.554 ± 0.999 mL. The post-treatment volume was significantly reduced compared with the pretreatment volume in all patients (P < 0.0001). Eighty-five percent of patients reported satisfaction with the improvement of skin laxity, and 80% of patients reported satisfaction with improvement of rhytids, such as the nasolabial folds. Side effects, such as epidermal burns and scar formation, were not observed throughout the study. Conclusion The advantages of this water-filtered near-infrared treatment are its high efficacy for skin tightening, associated with a minimal level of discomfort and minimal side effects. Together, these characteristics facilitate our ability to administer repeated treatments and provide alternative or adjunctive treatment for patients, with improved results. This study provides a qualitative and quantitative volumetric assessment, establishing the ability of this technology to reduce volume through noninvasive skin tightening. PMID:23837000

  11. Advanced Plant Habitat Test Harvest

    NASA Image and Video Library

    2017-08-24

    John "JC" Carver, a payload integration engineer with NASA Kennedy Space Center's Test and Operations Support Contract, places Arabidopsis thaliana plants harvested from the Advanced Plant Habitat (APH) Flight Unit No. 1 into an Ultra-low Freezer chilled to -150 degrees Celsius. The harvest is part of an ongoing verification test of the APH unit, which is located inside the International Space Station Environmental Simulator in Kennedy's Space Station Processing Facility. The APH undergoing testing at Kennedy is identical to one on the station and uses red, green and broad-spectrum white LED lights to grow plants in an environmentally controlled chamber. The seeds grown during the verification test will be grown on the station to help scientists understand how these plants adapt to spaceflight.

  12. Storage and Viability Assessment of Date Palm Pollen.

    PubMed

    Maryam; Jaskani, Muhammad J; Naqvi, Summar A

    2017-01-01

    Pollen storage and viability are very important for pollination, breeding, biodiversity, biotechnology, conservation, and other biological and non-biological studies of the date palm. Optimizing procedures and duration of storage are important for effective and long-term date palm pollen storage and viability. Here we describe pollen storage methods, such as room temperature (25-30 °C), refrigeration (4 °C), storage at 4 °C in desiccators, deep freezer (-20 °C), and cryopreservation (-196 °C). Based on pollen viability by staining and in vitro germination methods, cryopreservation is the best method for long-term storage without any significant effect on pollen viability (75-84%); however, the percentage of pollen viability depends on the storage period.

  13. STS-128 crew visits Stennis

    NASA Technical Reports Server (NTRS)

    2009-01-01

    Astronauts C.J. Sturckow (seated, left) and Pat Forrester (seated, right) sign autographs during their Oct. 7 visit to Stennis Space Center. The astronauts visited the rocket engine testing facility to thank Stennis employees for contributions to their recent STS-128 space shuttle mission. All three of the main engines used on the mission were tested at Stennis. Sturckow served as commander for the STS-128 flight; Forrester was a mission specialist. During a 14-day mission aboard space shuttle discovery, the STS-128 crew delivered equipment and supplies to the International Space Station, including science and storage racks, a freezer to store research samples, a new sleeping compartment and an exercise treadmill. The mission featured three spacewalks to replace experiments and install new equipment at the space station.

  14. Kitchen Physics: Lessons in Fluid Pressure and Error Analysis

    NASA Astrophysics Data System (ADS)

    Vieyra, Rebecca Elizabeth; Vieyra, Chrystian; Macchia, Stefano

    2017-02-01

    Although the advent and popularization of the "flipped classroom" tends to center around at-home video lectures, teachers are increasingly turning to at-home labs for enhanced student engagement. This paper describes two simple at-home experiments that can be accomplished in the kitchen. The first experiment analyzes the density of four liquids using a waterproof case and a smartphone barometer in a container, sink, or tub. The second experiment determines the relationship between pressure and temperature of an ideal gas in a constant volume container placed momentarily in a refrigerator freezer. These experiences provide a ripe opportunity both for learning fundamental physics concepts as well as to investigate a variety of error analysis techniques that are frequently overlooked in introductory physics courses.

  15. Determination of properties of PVE lubricants with HFC refrigerants[PolyVinylEther

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kaneko, Masato; Sakanoue, Shuichi; Tazaki, Toshihiro

    1999-07-01

    Polyalkyleneglycol (PAG) and polyol ester (POE) have been developed as refrigeration lubricants, used with HFC134a. PAG is used for automotive air conditioning systems and POE is used for domestic reciprocating refrigerators and for A/C systems. Although PAG exhibits good lubricity performance, it is difficult to use for domestic reciprocating refrigerators due to its low dielectric property. POE is difficult to use for automotive A/C systems, due to hydrolysis and poor lubricity performance. Polyvinylether (PVE) can be used in place of PAG and POE with HFC refrigerants. PVE is used for A/C systems as well as refrigerator and freezer applications. PVEmore » is an ideal lubricant for use with HFCs.« less

  16. KSC-2009-4354

    NASA Image and Video Library

    2009-07-31

    CAPE CANAVERAL, Fla. – On Launch Pad 39A at NASA's Kennedy Space Center in Florida, the payload canister is lifted up to the Payload Changeout Room in the rotating service structure. Umbilical lines that keep the payload in an environmentally controlled environment are still attached. Inside is the payload for space shuttle Discovery and the STS-128 mission, the Multi-Purpose Logistics Module Leonardo and the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery's 13-day flight will deliver a new crew member and 33,000 pounds of equipment to the station. The equipment includes science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch of Discovery on its STS-128 mission is targeted for August 25. Photo credit: NASA/Kim Shiflett

  17. KSC-2009-4352

    NASA Image and Video Library

    2009-07-31

    CAPE CANAVERAL, Fla. – On Launch Pad 39A at NASA's Kennedy Space Center in Florida, the payload canister is lifted up to the Payload Changeout Room in the rotating service structure. Umbilical lines that keep the payload in an environmentally controlled environment are still attached. Inside is the payload for space shuttle Discovery and the STS-128 mission, the Multi-Purpose Logistics Module Leonardo and the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery's 13-day flight will deliver a new crew member and 33,000 pounds of equipment to the station. The equipment includes science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch of Discovery on its STS-128 mission is targeted for August 25. Photo credit: NASA/Kim Shiflett

  18. KSC-2009-4355

    NASA Image and Video Library

    2009-07-31

    CAPE CANAVERAL, Fla. – On Launch Pad 39A at NASA's Kennedy Space Center in Florida, the payload canister is lifted up to the Payload Changeout Room in the rotating service structure. Umbilical lines that keep the payload in an environmentally controlled environment are still attached. Inside is the payload for space shuttle Discovery and the STS-128 mission, the Multi-Purpose Logistics Module Leonardo and the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery's 13-day flight will deliver a new crew member and 33,000 pounds of equipment to the station. The equipment includes science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch of Discovery on its STS-128 mission is targeted for August 25. Photo credit: NASA/Kim Shiflett

  19. KSC-2009-4353

    NASA Image and Video Library

    2009-07-31

    CAPE CANAVERAL, Fla. – On Launch Pad 39A at NASA's Kennedy Space Center in Florida, the payload canister is lifted up to the Payload Changeout Room in the rotating service structure. Umbilical lines that keep the payload in an environmentally controlled environment are still attached. Inside is the payload for space shuttle Discovery and the STS-128 mission, the Multi-Purpose Logistics Module Leonardo and the Lightweight Multi-Purpose Experiment Support Structure Carrier. Discovery's 13-day flight will deliver a new crew member and 33,000 pounds of equipment to the station. The equipment includes science and storage racks, a freezer to store research samples, a new sleeping compartment and the COLBERT treadmill. Launch of Discovery on its STS-128 mission is targeted for August 25. Photo credit: NASA/Kim Shiflett

  20. Field testing energy-saving hermetic compressors in residential refrigerators

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sauber, R.S.; Middleton, M.G.

    The design of an energy-saving compressor for low back pressure applications is reviewed. Calorimeter performance results are stated for two sizes of the efficient design and compared with performance test results for a standard compressor. Power consumption of a refrigerator-freezer is given with a standard compressor and with the energy-saving compressor. The preparation of the refrigerators used in the field test is discussed, along with the criteria used in selecting the instrumentation for the project. Results of the energy-saving compressor in the field test, along with a comparison to a standard production compressor, are presented. Some conclusions are drawn, basedmore » on the data, in relation to important factors in residential refrigerator power consumption.« less

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