Multielectron effects in the photoelectron momentum distribution of noble-gas atoms driven by visible-to-infrared-frequency laser pulses: A time-dependent density-functional-theory approach

NASA Astrophysics Data System (ADS)

Murakami, Mitsuko; Zhang, G. P.; Chu, Shih-I.

2017-05-01

We present the photoelectron momentum distributions (PMDs) of helium, neon, and argon atoms driven by a linearly polarized, visible (527-nm) or near-infrared (800-nm) laser pulse (20 optical cycles in duration) based on the time-dependent density-functional theory (TDDFT) under the local-density approximation with a self-interaction correction. A set of time-dependent Kohn-Sham equations for all electrons in an atom is numerically solved using the generalized pseudospectral method. An effect of the electron-electron interaction driven by a visible laser field is not recognizable in the helium and neon PMDs except for a reduction of the overall photoelectron yield, but there is a clear difference between the PMDs of an argon atom calculated with the frozen-core approximation and TDDFT, indicating an interference of its M -shell wave functions during the ionization. Furthermore, we find that the PMDs of degenerate p states are well separated in intensity when driven by a near-infrared laser field, so that the single-active-electron approximation can be adopted safely.

On the role of the frozen surface approximation in small wave-height perturbation theory for moving surfaces

NASA Astrophysics Data System (ADS)

Keiffer, Richard; Novarini, Jorge; Scharstein, Robert

2002-11-01

In the standard development of the small wave-height approximation (SWHA) perturbation theory for scattering from moving rough surfaces [e.g., E. Y. Harper and F. M. Labianca, J. Acoust. Soc. Am. 58, 349-364 (1975)] the necessity for any sort of frozen surface approximation is avoided by the replacement of the rough boundary by a flat (and static) boundary. In this paper, this seemingly fortuitous byproduct of the small wave-height approximation is examined and found to fail to fully agree with an analysis based on the kinematics of the problem. Specifically, the first-order correction term from standard perturbation approach predicts a scattered amplitude that depends on the source frequency, whereas the kinematics of the problem point to a scattered amplitude that depends on the scattered frequency. It is shown that a perturbation approach in which an explicit frozen surface approximation is made before the SWHA is invoked predicts (first-order) scattered amplitudes that are in agreement with the kinematic analysis. [Work supported by ONR/NRL (PE 61153N-32) and by grants of computer time DoD HPC Shared Resource Center at Stennis Space Center, MS.

Orbital dependent functionals: An atom projector augmented wave method implementation

NASA Astrophysics Data System (ADS)

Xu, Xiao

This thesis explores the formulation and numerical implementation of orbital dependent exchange-correlation functionals within electronic structure calculations. These orbital-dependent exchange-correlation functionals have recently received renewed attention as a means to improve the physical representation of electron interactions within electronic structure calculations. In particular, electron self-interaction terms can be avoided. In this thesis, an orbital-dependent functional is considered in the context of Hartree-Fock (HF) theory as well as the Optimized Effective Potential (OEP) method and the approximate OEP method developed by Krieger, Li, and Iafrate, known as the KLI approximation. In this thesis, the Fock exchange term is used as a simple well-defined example of an orbital-dependent functional. The Projected Augmented Wave (PAW) method developed by P. E. Blochl has proven to be accurate and efficient for electronic structure calculations for local and semi-local functions because of its accurate evaluation of interaction integrals by controlling multiple moments. We have extended the PAW method to treat orbital-dependent functionals in Hartree-Fock theory and the Optimized Effective Potential method, particularly in the KLI approximation. In the course of study we develop a frozen-core orbital approximation that accurately treats the core electron contributions for above three methods. The main part of the thesis focuses on the treatment of spherical atoms. We have investigated the behavior of PAW-Hartree Fock and PAW-KLI basis, projector, and pseudopotential functions for several elements throughout the periodic table. We have also extended the formalism to the treatment of solids in a plane wave basis and implemented PWPAW-KLI code, which will appear in future publications.

Does the 4f-shell contribute to bonding in tetravalent lanthanide halides?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ji, Wen-Xin; School of Chemistry and Chemical Engineering, Ningxia University, 750015 Yinchuan; Xu, Wei

2014-12-28

Lanthanide tetrahalide molecules LnX{sub 4} (Ln = Ce, Pr, Tb; X = F, Cl, Br, I) have been investigated by density functional theory at the levels of the relativistic Zero Order Regular Approximation and the relativistic energy-consistent pseudopotentials, using frozen small- and medium-cores. The calculated bond lengths and vibrational frequencies are close to the experimental data. Our calculations indicate 4f shell contributions to bonding in LnX{sub 4}, in particular for the early lanthanides, which show significant overlap between the Ln 4f-shell and the halogen np-shells. The 4f shells contribute to Ln-X bonding in LnX{sub 4} about one third more thanmore » in LnX{sub 3}.« less

Climatic change and evaporative processes in the development of Common Era hypersaline lakes, East Antarctica: A study of Lake Suribati

NASA Astrophysics Data System (ADS)

Nakashima, H.; Seto, K.; Katsuki, K.; Kaneko, H.; yamada, K.; Imura, S.; Dettman, D. L.

2011-12-01

The Antarctic continent was uplifted by glacioisostatic rebound due to the regression of ice sheets after the last glacial period. Today's saline lakes were formed in shallow basins originally below sea level. Antarctic hypersaline lakes are formed by concentration of isolated seawater bodies as affected by recent climate change. Many saline lakes are found in the ice-free area of the Soya coast, East Antarctica. Lake Suribati is located in Sukarvsnes on the Soya coast. It is a hypersaline lake with maximum salinity ~200 psu, and an observable stable halocline at 7~12m depth. This study uses Lake Suribati sediment core Sr4C-01, collected by the 46th Japanese Antarctica Research Expedition, to examine the relationship of climatic change to evaporative processes and solute concentration in Lake Suribati in the Common Era. Sr4C-01 core was collected at 9.53m water depth in Lake Suribati in 2005 (core length is 63cm). This core primarily consists of black mud and laminated black organic mud. In the interval from 10 to 24cm below the sediment surface evaporite crystals occur. The age of the Sr4C-01 core bottom is estimated to be ~3,500 cal yrs BP, based on AMS carbon-14 dating at 6 core horizons. The evaporite crystals were indentified as aragonite based on XRD. Total inorganic carbon (TIC) content is low, around 0.5%, throughout the Sr4C-01 core, with higher values, approximately 1~4%, in two intervals, 57~52cm and 29~10cm core depth. Variation in CaO content tracks TIC content. We suggest that synchronous change in CaO and TIC contents indicate the vertical change in the amount of aragonite. Two intervals of evaporite precipition imply two intervals of evaporation and concentration of lake water. Hypersaline lake conditions did not occur soon after the isolation from the sea, rather these occurred under repeated concentration and dilution of lake water. Dilution of saline lake water could occur through the inflow of melt water from local snow or ice, indicating a warm climate interval. During cool periods, local snow and ice sheet may have remained frozen. In this case, lake water volume would decrease by sublimation from the frozen lake surface, leading to salt concentration. Based on MgO and Na2O content data, we suggest that other Mg and Na evaporites occur in the core. If such evaporates can be identified, a detailed solute concentration process can be described. Analysis of evaporites in sediment core from Antarctic hypersaline lakes have great potential as proxy indicators for the study of climate change in Antarctica.

STEMsalabim: A high-performance computing cluster friendly code for scanning transmission electron microscopy image simulations of thin specimens.

PubMed

Oelerich, Jan Oliver; Duschek, Lennart; Belz, Jürgen; Beyer, Andreas; Baranovskii, Sergei D; Volz, Kerstin

2017-06-01

We present a new multislice code for the computer simulation of scanning transmission electron microscope (STEM) images based on the frozen lattice approximation. Unlike existing software packages, the code is optimized to perform well on highly parallelized computing clusters, combining distributed and shared memory architectures. This enables efficient calculation of large lateral scanning areas of the specimen within the frozen lattice approximation and fine-grained sweeps of parameter space. Copyright © 2017 Elsevier B.V. All rights reserved.

Geotechnical properties of core sample from methane hydrate deposits in Eastern Nankai Trough

NASA Astrophysics Data System (ADS)

Yoneda, J.; Masui, A.; Egawa, K.; Konno, Y.; Ito, T.; Kida, M.; Jin, Y.; Suzuki, K.; Nakatsuka, Y.; Tenma, N.; Nagao, J.

2013-12-01

To date, MH extraction has been simulated in several ways to help ensure the safe and efficient production of gas, with a particular focus on the investigation of landsliding, uneven settlement, and production well integrity. The mechanical properties of deep sea sediments and gas-hydrate-bearing sediments, typically obtained through material tests, are essential for the geomechanical response simulation to hydrate extraction. We conducted triaxial compression tests and the geotechnical properties of the sediments was investigated. Consolidated undrained compression tests were performed for silty sediments. And consolidated drained tests were performed for sandy samples. In addition, permeability was investigated from isotropic consolidation results. These core samples recovered from methane hydrate deposits of Daini Atsumi Knoll in Eastern Nankai Trough during the 2012 JOGMEC/JAPEX Pressure coring operation. The pressure core samples were rapidly depressurized on the ship and it were frozen using liquid nitrogen to prevent MH dissociation. Undrained shear strength of the core samples increase linearly with depth from sea floor. These core samples should be normally consolidated sample in-situ. Drained shear strength increases dramatically with hydrate saturation increases. Peak stress ratio q/p' of the core sample which has 73% of hydrate saturation was approximately 2.0 and it decrease down to 1.3 at the critical state. Dilatancy also changed from compressive tendency to dilative tendency with hydrate saturation increase. This study was financially supported by the Research Consortium for Methane Hydrate Resources in Japan (MH21 Research Consortium) that carries out Japan's Methane Hydrate R&D Program conducted by the Ministry of Economy, Trade and Industry (METI).

77 FR 471 - Emergency Food Assistance Program; Availability of Foods for Fiscal Year 2012

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-05

..., poultry, fish, vegetables, dry beans, juices, and fruits. Approximately $37.5 million in surplus foods..., frozen ham, frozen turkey roast, blackeye beans, garbanzo beans, great northern beans, light red kidney beans, lentils, lima beans, pinto beans, egg mix, shell eggs, lowfat bakery mix, egg noodles, white and...

78 FR 7750 - Emergency Food Assistance Program; Availability of Foods for Fiscal Year 2013

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-04

..., fish, vegetables, dry beans, juices, and fruits. Approximately $274.5 million in surplus foods acquired in FY 2012 are being delivered to States in FY 2013. These foods include beans (dried, canned..., frozen ham, frozen turkey roast, blackeye beans, garbanzo beans, great northern beans, light red kidney...

CT Scans of Cores Metadata, Barrow, Alaska 2015

DOE Data Explorer

Katie McKnight; Tim Kneafsey; Craig Ulrich

2015-03-11

Individual ice cores were collected from Barrow Environmental Observatory in Barrow, Alaska, throughout 2013 and 2014. Cores were drilled along different transects to sample polygonal features (i.e. the trough, center and rim of high, transitional and low center polygons). Most cores were drilled around 1 meter in depth and a few deep cores were drilled around 3 meters in depth. Three-dimensional images of the frozen cores were constructed using a medical X-ray computed tomography (CT) scanner. TIFF files can be uploaded to ImageJ (an open-source imaging software) to examine soil structure and densities within each core.

Treatment of Articular Cartilage Defects in the Goat with Frozen Versus Fresh Osteochondral Allografts: Effects on Cartilage Stiffness, Zonal Composition, and Structure at Six Months

PubMed Central

Pallante, Andrea L.; Görtz, Simon; Chen, Albert C.; Healey, Robert M.; Chase, Derek C.; Ball, Scott T.; Amiel, David; Sah, Robert L.; Bugbee, William D.

2012-01-01

Background: Understanding the effectiveness of frozen as compared with fresh osteochondral allografts at six months after surgery and the resultant consequences of traditional freezing may facilitate in vivo maintenance of cartilage integrity. Our hypothesis was that the state of the allograft at implantation affects its performance after six months in vivo. Methods: The effect of frozen as compared with fresh storage on in vivo allograft performance was determined for osteochondral allografts that were transplanted into seven recipient goats and analyzed at six months. Allograft performance was assessed by examining osteochondral structure (cartilage thickness, fill, surface location, surface degeneration, and bone-cartilage interface location), zonal cartilage composition (cellularity, matrix content), and cartilage biomechanical function (stiffness). Relationships between cartilage stiffness or cartilage composition and surface degeneration were assessed with use of linear regression. Results: Fresh allografts maintained cartilage load-bearing function, while also maintaining zonal organization of cartilage cellularity and matrix content, compared with frozen allografts. Overall, allograft performance was similar between fresh allografts and nonoperative controls. However, cartilage stiffness was approximately 80% lower (95% confidence interval [CI], 73% to 87%) in the frozen allografts than in the nonoperative controls or fresh allografts. Concomitantly, in frozen allografts, matrix content and cellularity were approximately 55% (95% CI, 22% to 92%) and approximately 96% (95% CI, 94% to 99%) lower, respectively, than those in the nonoperative controls and fresh allografts. Cartilage stiffness correlated positively with cartilage cellularity and matrix content, and negatively with surface degeneration. Conclusions: Maintenance of cartilage load-bearing function in allografts is associated with zonal maintenance of cartilage cellularity and matrix content. In this animal model, frozen allografts displayed signs of failure at six months, with cartilage softening, loss of cells and matrix, and/or graft subsidence, supporting the importance of maintaining cell viability during allograft storage and suggesting that outcomes at six months may be indicative of long-term (dys)function. Clinical Relevance: Fresh versus frozen allografts represent the “best versus worst” conditions with respect to chondrocyte viability, but “difficult versus simple” with respect to acquisition and distribution. The outcomes described from these two conditions expand the current understanding of in vivo cartilage remodeling and describe structural properties (initial graft subsidence), which may have implications for impending graft failure. PMID:23138239

Effect of gamma-irradiation on frozen shrimps for decontamination of pathogenic bacteria

NASA Astrophysics Data System (ADS)

Ito, Hitoshi; Rashid, Harun Or; Sangthong, Naruemon; Adulyatham, Pitaya; Rattagool, Pongpen; Ishigaki, Isao

1993-07-01

Twelve samples of imported frozen shrimps were used in this study. The total aerobic bacteria were at 2 × 10 4 to 6 × 10 6 per gram. A few of Vibrio parahaemolyticus, V. mimicus, V. alginolyticus, V. vulnificus, V. fluvialis and Listeria monocytogenes were isolated from many samples. However, Salmonella was not detected in any of the samples. After exposure to 4-5 kGy of gamma-rays, the total aerobic bacteria in frozen shrimps were reduced by approximately 2-3 log cycles. The dose necessary to reduce the vibrio isolates and Aeromonas hydrophila at a level of below 10 -4 per gram was about 3 kGy in frozen shrimps, whereas about 3.5 kGy was required for L. monocytogenes and Salmonella typhimurium. In this study, unpleasant off-odor was clearly detected in the non-frozen shrimps irradiated at 2.5 kGy. On the other hand, off-odor was negligible in the frozen product below 5 kGy irradiation. No remarkable changes of peroxide values were also obtained up to 9 kGy of irradiation in the frozen shrimps. However peroxide values of non-frozen shrimps were clearly increased even irradiated at 4 kGy. Trimethylamine content was not changed at doses below 10 kGy in both of frozen and non-frozen shrimps. Shelf-life of defrosted shrimps were extended ca. 2 times under non-frozen market conditions.

Derivation of general analytic gradient expressions for density-fitted post-Hartree-Fock methods: An efficient implementation for the density-fitted second-order Møller–Plesset perturbation theory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bozkaya, Uğur, E-mail: ugur.bozkaya@atauni.edu.tr

General analytic gradient expressions (with the frozen-core approximation) are presented for density-fitted post-HF methods. An efficient implementation of frozen-core analytic gradients for the second-order Møller–Plesset perturbation theory (MP2) with the density-fitting (DF) approximation (applying to both reference and correlation energies), which is denoted as DF-MP2, is reported. The DF-MP2 method is applied to a set of alkanes, conjugated dienes, and noncovalent interaction complexes to compare the computational cost of single point analytic gradients with MP2 with the resolution of the identity approach (RI-MP2) [F. Weigend and M. Häser, Theor. Chem. Acc. 97, 331 (1997); R. A. Distasio, R. P. Steele,more » Y. M. Rhee, Y. Shao, and M. Head-Gordon, J. Comput. Chem. 28, 839 (2007)]. In the RI-MP2 method, the DF approach is used only for the correlation energy. Our results demonstrate that the DF-MP2 method substantially accelerate the RI-MP2 method for analytic gradient computations due to the reduced input/output (I/O) time. Because in the DF-MP2 method the DF approach is used for both reference and correlation energies, the storage of 4-index electron repulsion integrals (ERIs) are avoided, 3-index ERI tensors are employed instead. Further, as in case of integrals, our gradient equation is completely avoid construction or storage of the 4-index two-particle density matrix (TPDM), instead we use 2- and 3-index TPDMs. Hence, the I/O bottleneck of a gradient computation is significantly overcome. Therefore, the cost of the generalized-Fock matrix (GFM), TPDM, solution of Z-vector equations, the back transformation of TPDM, and integral derivatives are substantially reduced when the DF approach is used for the entire energy expression. Further application results show that the DF approach introduce negligible errors for closed-shell reaction energies and equilibrium bond lengths.« less

Defective Reduction in Frozen Pie Manufacturing Process

NASA Astrophysics Data System (ADS)

Nooted, Oranuch; Tangjitsitcharoen, Somkiat

2017-06-01

The frozen pie production has a lot of defects resulting in high production cost. Failure mode and effect analysis (FMEA) technique has been applied to improve the frozen pie process. Pareto chart is also used to determine the major defects of frozen pie. There are 3 main processes that cause the defects which are the 1st freezing to glazing process, the forming process, and the folding process. The Risk Priority Number (RPN) obtained from FMEA is analyzed to reduce the defects. If RPN of each cause exceeds 45, the process will be considered to be improved and selected for the corrective and preventive actions. The results showed that RPN values decreased after the correction. Therefore, the implementation of FMEA technique can help to improve the performance of frozen pie process and reduce the defects approximately 51.9%.

Analytical study of comet nucleus samples

NASA Technical Reports Server (NTRS)

Albee, A. L.

1989-01-01

Analytical procedures for studying and handling frozen (130 K) core samples of comet nuclei are discussed. These methods include neutron activation analysis, x ray fluorescent analysis and high resolution mass spectroscopy.

How will the future warming climate impact the river discharge in the alpine mountain region of upper Heihe River Basin?

NASA Astrophysics Data System (ADS)

Wang, Y.; Yang, H.; Yang, D.; Gao, B.; Qin, Y.

2017-12-01

The Tibetan Plateau is more sensitive to the global climate change than other areas due to its special geography. Previous studies have shown that, besides the changes of temperature and precipitation, the changes in the cryosphere such as glacier and frozen ground also have important and far-reaching effects on the ecological and hydrological processes in the basin. In order to reliably predict the future runoff changing trend in the future, it's important to estimate the responses of cryosphere to the future climate change, as well as its impacts on the hydrological processes. Based on typical future climate scenarios (under emission scenario RCP4.5) from five general circulation models (GCMs) and one regional climate model (RCM), as well as a distributed eco-hydrological model (GBEHM), this study analyzes the possible future climate change (from 2011 to 2060) and its impacts on cryospheric and hydrological processes in upper Heihe River Basin, a typical cold mountain region located in the Northeast Tibetan Plateau. The results suggest that air temperature is expected to rise in the future by approximately 0.32 °C/10a, and precipitation is expected to rise slightly by about 3 mm/10a. Under the rising air temperature, the maximum frozen depth of seasonally frozen ground will decrease by about 4.1 cm/10a and the active layer depth of the frozen ground will increase by about 6.2 cm/10a. The runoff is expected to reduce by approximately 6 mm/10a and the evapotranspiration is expected to increase by approximately 9 mm/10a. These changes in hydrological processes are mainly caused by the air temperature rise. The impacts of air temperature change on the hydrological processes are mainly due to the changes of frozen ground. The thickening of active layer of the frozen ground increases the soil storage capacity, leading to the decrease of runoff and increase of evapotranspiration. Results show that, when the active layer depth increase by 1 cm, the runoff will decrease by about 1 2 mm and the evapotranspiration will increase by about 0.7 2 mm. Additionally, the changes from permafrost to seasonal frozen ground increase the groundwater infiltration, which also leads to the decrease of surface runoff.

Aminostratigraphy of Organisms in Antarctic and Siberian Permafrost Cores

NASA Technical Reports Server (NTRS)

Brinton, K. L. F.; Tsapin, A. I.; McDonald, G. D.; Gilichinsky, D.

1999-01-01

Amino acid racemization dating (or aminostratigraphy) in Antarctic and Siberian permafrost core samples can be used to evaluate the age of organisms in frozen environments. The potential for subsurface permafrost on Mars makes terrestrial permafrost an important source of information regarding the preservation of both living organisms and their remains. Additional information is contained in the original extended abstract.

Phylogenetic analysis of anaerobic psychrophilic enrichment cultures obtained from a greenland glacier ice core

NASA Technical Reports Server (NTRS)

Sheridan, Peter P.; Miteva, Vanya I.; Brenchley, Jean E.

2003-01-01

The examination of microorganisms in glacial ice cores allows the phylogenetic relationships of organisms frozen for thousands of years to be compared with those of current isolates. We developed a method for aseptically sampling a sediment-containing portion of a Greenland ice core that had remained at -9 degrees C for over 100,000 years. Epifluorescence microscopy and flow cytometry results showed that the ice sample contained over 6 x 10(7) cells/ml. Anaerobic enrichment cultures inoculated with melted ice were grown and maintained at -2 degrees C. Genomic DNA extracted from these enrichments was used for the PCR amplification of 16S rRNA genes with bacterial and archaeal primers and the preparation of clone libraries. Approximately 60 bacterial inserts were screened by restriction endonuclease analysis and grouped into 27 unique restriction fragment length polymorphism types, and 24 representative sequences were compared phylogenetically. Diverse sequences representing major phylogenetic groups including alpha, beta, and gamma Proteobacteria as well as relatives of the Thermus, Bacteroides, Eubacterium, and Clostridium groups were found. Sixteen clone sequences were closely related to those from known organisms, with four possibly representing new species. Seven sequences may reflect new genera and were most closely related to sequences obtained only by PCR amplification. One sequence was over 12% distant from its closest relative and may represent a novel order or family. These results show that phylogenetically diverse microorganisms have remained viable within the Greenland ice core for at least 100,000 years.

Phylogenetic Analysis of Anaerobic Psychrophilic Enrichment Cultures Obtained from a Greenland Glacier Ice Core

PubMed Central

Sheridan, Peter P.; Miteva, Vanya I.; Brenchley, Jean E.

2003-01-01

The examination of microorganisms in glacial ice cores allows the phylogenetic relationships of organisms frozen for thousands of years to be compared with those of current isolates. We developed a method for aseptically sampling a sediment-containing portion of a Greenland ice core that had remained at −9°C for over 100,000 years. Epifluorescence microscopy and flow cytometry results showed that the ice sample contained over 6 × 107 cells/ml. Anaerobic enrichment cultures inoculated with melted ice were grown and maintained at −2°C. Genomic DNA extracted from these enrichments was used for the PCR amplification of 16S rRNA genes with bacterial and archaeal primers and the preparation of clone libraries. Approximately 60 bacterial inserts were screened by restriction endonuclease analysis and grouped into 27 unique restriction fragment length polymorphism types, and 24 representative sequences were compared phylogenetically. Diverse sequences representing major phylogenetic groups including alpha, beta, and gamma Proteobacteria as well as relatives of the Thermus, Bacteroides, Eubacterium, and Clostridium groups were found. Sixteen clone sequences were closely related to those from known organisms, with four possibly representing new species. Seven sequences may reflect new genera and were most closely related to sequences obtained only by PCR amplification. One sequence was over 12% distant from its closest relative and may represent a novel order or family. These results show that phylogenetically diverse microorganisms have remained viable within the Greenland ice core for at least 100,000 years. PMID:12676695

Effect of solvation-related interaction on the low-temperature dynamics of proteins

NASA Astrophysics Data System (ADS)

Zuo, Guanghong; Wang, Jun; Qin, Meng; Xue, Bin; Wang, Wei

2010-03-01

The effect of solvation-related interaction on the low-temperature dynamics of proteins is studied by taking into account the desolvation barriers in the interactions of native contacts. It is found out that about the folding transition temperature, the protein folds in a cooperative manner, and the water molecules are expelled from the hydrophobic core at the final stage in the folding process. At low temperature, however, the protein would generally be trapped in many metastable conformations with some water molecules frozen inside the protein. The desolvation takes an important role in these processes. The number of frozen water molecules and that of frozen states of proteins are further analyzed with the methods based on principal component analysis (PCA) and the clustering of conformations. It is found out that both the numbers of frozen water molecules and the frozen states of the protein increase quickly below a certain temperature. Especially, the number of frozen states of the protein increases exponentially following the decrease in the temperature, which resembles the basic features of glassy dynamics. Interestingly, it is observed that the freezing of water molecules and that of protein conformations happen at almost the same temperature. This suggests that the solvation-related interaction performs an important role for the low-temperature dynamics of the model protein.

Experimental Study and a Mathematical Model of the Processes in Frozen Soil Under a Reservoir with a Hot Heat-Transfer Agent

NASA Astrophysics Data System (ADS)

Kislitsyn, A. A.; Shastunova, U. Yu.; Yanbikova, Yu. F.

2018-05-01

On an experimental setup, the authors have measured temperature fields in frozen soil during the filling of a reservoir with hot heat-transfer agent (oil), and also the change in the shape and position of the front of ice melting (isotherms T = 0°C) with time. The approximate solution of a two-dimensional Stefan problem on thawing of frozen soil has been given; it has been shown that satisfactory agreement with experimental results can only be obtained with account taken of the convective transfer of heat due to the water motion in the region of thawed soil.

Experimental Study and a Mathematical Model of the Processes in Frozen Soil Under a Reservoir with a Hot Heat-Transfer Agent

NASA Astrophysics Data System (ADS)

Kislitsyn, A. A.; Shastunova, U. Yu.; Yanbikova, Yu. F.

2018-03-01

On an experimental setup, the authors have measured temperature fields in frozen soil during the filling of a reservoir with hot heat-transfer agent (oil), and also the change in the shape and position of the front of ice melting (isotherms T = 0°C) with time. The approximate solution of a two-dimensional Stefan problem on thawing of frozen soil has been given; it has been shown that satisfactory agreement with experimental results can only be obtained with account taken of the convective transfer of heat due to the water motion in the region of thawed soil.

A Study of the Shipment of Produce to Department of Defense Customers in Korea.

DTIC Science & Technology

1998-03-01

DSCP logistics planners can more effectively provide superior products and customer service to South Korean produce customers. 3 C. RESEARCH METHODOLOGY...an average of approximately twelve personnel and maintain responsibility for providing chilled and frozen products to their customers. Additionally...responsible for providing chill and frozen products to customers. 2. DSO-San Francisco The Defense Subsistence Office (DSO)-San Francisco is located in

Nature of electrogenerated intermediates in nitro-substituted nor-β-lapachones: the structure of radical species during successive electron transfer in multiredox centers.

PubMed

Armendáriz-Vidales, Georgina; Hernández-Muñoz, Lindsay S; González, Felipe J; de Souza, Antonio A; de Abreu, Fabiane C; Jardim, Guilherme A M; da Silva, Eufrânio N; Goulart, Marilia O F; Frontana, Carlos

2014-06-06

Electrochemical, spectroelectrochemical, and theoretical studies of the reduction reactions in nor-β-lapachone derivatives including a nitro redox center showed that reduction of the compounds involves the formation of several radical intermediates, including a biradical dianion resultant from the separate reduction of the quinone and nitro groups in the molecules. Theoretical descriptions of the corresponding Fukui functions f(αα)⁺ and f(ββ)⁺(r) and LUMO densities considering finite differences and frozen core approximations for describing the changes in electron and spin densities of the system allowed us to confirm these results. A description of the potential relationship with the obtained results and biological activity selectivity indexes suggests that both the formation of stable biradical dianion species and the stability of the semiquinone intermediates during further reduction are determining factors in the description of their biological activity.

(1 + 1) resonant enhanced multiphoton ionization via the A2Sigma(+) state of NO - Ionic rotational branching ratios and their intensity dependence

NASA Technical Reports Server (NTRS)

Rudolph, H.; Mckoy, V.; Dixit, S. N.; Huo, W. M.

1988-01-01

Rotational branching ratios resulting from the (1 + 1) resonant enhanced multiphoton ionization spectroscopy of NO via the 0-0 transition of the A-X band for the four possible branches that can be assigned as R(21.5) are explored using calculation performed in the frozen-core approximation at the Hartree-Fock level. The four different branches, of which three are distinctly different in the perturbative limit, have rather different branching ratios. The mixed R12 + Q22(21.5) branch, which is not intense and has the lowest transition energy, appears to give the best agreement with experimental branching ratio for parallel detection. The agreement is less satisfactory for perpendicular detection. Neither the effect of finite-acceptance angle of the photoelectron detector nor high intensities can explain the discrepancy.

Management of chickenpox with frozen mother's milk.

PubMed

Verd, Sergio; López, Esther

2012-08-01

If a mother has contracted chickenpox, the antibodies in her milk confer immunity against chickenpox to her breastfed babies. This passive immunization may avoid or spare the breastfed babies' symptoms of chickenpox. It is hypothesized that frozen breast milk may shorten chickenpox duration because specific antibodies against varicella zoster have been detected in human milk and they are resistant to digestion and are stable in frozen milk. The clinical outcomes of chickenpox in a 9-year-old boy and his father on frozen breast milk are reported. The study comprised a varicella-vaccine-refusing family attending a private office of pediatrics. The boy presented with a crusted varicella rash. The medical history revealed premature cessation of the typical varicella rash on day 3. It was coincidental with a supply of frozen human milk by his mother. Next, the father (41 years old) of this patient contracted chickenpox: he was on frozen breast milk from day 2, and no new pox emerged thereafter. The rash spread and numbered 50 to 150 lesions on day 2. Instead, the typical rash was expected to appear in three successive crops of lesions throughout the first week. The disease usually numbers approximately 250-500 lesions in unvaccinated healthy persons. Frozen breast milk may shorten chickenpox duration.

Microphysical properties of frozen particles inferred from Global Precipitation Measurement (GPM) Microwave Imager (GMI) polarimetric measurements

NASA Astrophysics Data System (ADS)

Gong, Jie; Wu, Dong L.

2017-02-01

Scattering differences induced by frozen particle microphysical properties are investigated, using the vertically (V) and horizontally (H) polarized radiances from the Global Precipitation Measurement (GPM) Microwave Imager (GMI) 89 and 166 GHz channels. It is the first study on frozen particle microphysical properties on a global scale that uses the dual-frequency microwave polarimetric signals.From the ice cloud scenes identified by the 183.3 ± 3 GHz channel brightness temperature (Tb), we find that the scattering by frozen particles is highly polarized, with V-H polarimetric differences (PDs) being positive throughout the tropics and the winter hemisphere mid-latitude jet regions, including PDs from the GMI 89 and 166 GHz TBs, as well as the PD at 640 GHz from the ER-2 Compact Scanning Submillimeter-wave Imaging Radiometer (CoSSIR) during the TC4 campaign. Large polarization dominantly occurs mostly near convective outflow regions (i.e., anvils or stratiform precipitation), while the polarization signal is small inside deep convective cores as well as at the remote cirrus region. Neglecting the polarimetric signal would easily result in as large as 30 % error in ice water path retrievals. There is a universal bell curve in the PD-TBV relationship, where the PD amplitude peaks at ˜ 10 K for all three channels in the tropics and increases slightly with latitude (2-4 K). Moreover, the 166 GHz PD tends to increase in the case where a melting layer is beneath the frozen particles aloft in the atmosphere, while 89 GHz PD is less sensitive than 166 GHz to the melting layer. This property creates a unique PD feature for the identification of the melting layer and stratiform rain with passive sensors.Horizontally oriented non-spherical frozen particles are thought to produce the observed PD because of different ice scattering properties in the V and H polarizations. On the other hand, turbulent mixing within deep convective cores inevitably promotes the random orientation of these particles, a mechanism that works effectively in reducing the PD. The current GMI polarimetric measurements themselves cannot fully disentangle the possible mechanisms.

Microphysical Properties of Frozen Particles Inferred from Global Precipitation Measurement (GPM) Microwave Imager (GMI) Polarimetric Measurements

NASA Technical Reports Server (NTRS)

Gong, Jie; Wu, Dongliang

2017-01-01

Scattering differences induced by frozen particle microphysical properties are investigated, using the vertically (V) and horizontally (H) polarized radiances from the Global Precipitation Measurement (GPM) Microwave Imager (GMI) 89 and 166GHz channels. It is the first study on global frozen particle microphysical properties that uses the dual-frequency microwave polarimetric signals. From the ice cloud scenes identified by the 183.3 3GHz channel brightness temperature (TB), we find that the scatterings of frozen particles are highly polarized with V-H polarimetric differences (PD) being positive throughout the tropics and the winter hemisphere mid-latitude jet regions, including PDs from the GMI 89 and 166GHz TBs, as well as the PD at 640GHz from the ER-2 Compact Scanning Submillimeter-wave Imaging Radiometer (CoSSIR) during the TC4 campaign. Large polarization dominantly occurs mostly near convective outflow region (i.e., anvils or stratiform precipitation), while the polarization signal is small inside deep convective cores as well as at the remote cirrus region. Neglecting the polarimetric signal would result in as large as 30 error in ice water path retrievals. There is a universal bell-curve in the PD TB relationship, where the PD amplitude peaks at 10K for all three channels in the tropics and increases slightly with latitude. Moreover, the 166GHz PD tends to increase in the case where a melting layer is beneath the frozen particles aloft in the atmosphere, while 89GHz PD is less sensitive than 166GHz to the melting layer. This property creates a unique PD feature for the identification of the melting layer and stratiform rain with passive sensors. Horizontally oriented non-spherical frozen particles are thought to produce the observed PD because of different ice scattering properties in the V and H polarizations. On the other hand, changes in the ice microphysical habitats or orientation due to turbulence mixing can also lead to a reduced PD in the deep convective cores. The current GMI polarimetric measurements themselves cannot fully disentangle the possible mechanisms.

Presence and dehydration of ikaite, calcium carbonate hexahydrate, in frozen shrimp shell.

PubMed

Mikkelsen, A; Andersen, A B; Engelsen, S B; Hansen, H C; Larsen, O; Skibsted, L H

1999-03-01

Ikaite, calcium carbonate hexahydrate, has by means of X-ray diffraction analyses of frozen samples been identified as the mineral component of the white spots formed in the shell of frozen shrimp during storage. When the shrimp thaw and the shell material is dried and kept at room temperature, ikaite rapidly transforms into a mixture of anhydrous calcium carbonate forms. X-ray diffraction analyses and Raman spectra of synthetic ikaite as well as the dehydration product confirm the assignments, and the rate constant for dehydration is approximately 7 x 10(-)(4) s(-)(1) at ambient temperature. Differential scanning calorimetry showed that dehydration of synthetic ikaite is an entropy-driven, athermal process and confirms that a single first-order reaction is rate-determining. Ikaite is found to be stable in aqueous solution at temperatures below 5 degrees C and in the shell of frozen shrimps but decomposes on thawing to form anhydrous calcium carbonates.

Ensemble Space-Time Correlation of Plasma Turbulence in the Solar Wind.

PubMed

Matthaeus, W H; Weygand, J M; Dasso, S

2016-06-17

Single point measurement turbulence cannot distinguish variations in space and time. We employ an ensemble of one- and two-point measurements in the solar wind to estimate the space-time correlation function in the comoving plasma frame. The method is illustrated using near Earth spacecraft observations, employing ACE, Geotail, IMP-8, and Wind data sets. New results include an evaluation of both correlation time and correlation length from a single method, and a new assessment of the accuracy of the familiar frozen-in flow approximation. This novel view of the space-time structure of turbulence may prove essential in exploratory space missions such as Solar Probe Plus and Solar Orbiter for which the frozen-in flow hypothesis may not be a useful approximation.

Frozen yogurt and ice cream were less healthy than yogurt, and adding toppings reduced their nutrition value: evidence from 1999-2014 National Health and Nutrition Examination Survey.

PubMed

An, Ruopeng; Jiang, Ning

2017-06-01

It was hypothesized that frozen yogurt and ice cream would be less healthy than yogurt. We examined daily energy and nutrient intake from yogurt, frozen yogurt, and ice cream among US adults. In-person 24-hour dietary recall data (n=6453) came from the 1999-2014 National Health and Nutrition Examination Survey. Regression analyses were performed to examine the differences in energy/nutrient intake from frozen yogurt and ice cream (with/without toppings) in comparison to yogurt. Approximately 5.3%, 0.9%, and 14.3% of US adults consumed yogurt, frozen yogurt, and ice cream on any given day, respectively. Among frozen yogurt and ice cream consumers, 29.7% and 14.8% added toppings to their consumption, respectively. Compared with yogurt, frozen yogurt consumption with and without toppings was associated with increased daily energy intake by 214.6 and 97.9kj, respectively; whereas ice cream consumption with and without toppings was associated with increased daily energy intake by 427.2 and 343.5kj, respectively. Compared with yogurt, frozen yogurt consumption was associated with a decreased intake of most vitamins/minerals under examination, but increased intake of sugar, total/saturated fat, cholesterol, fiber, and iron. Adding toppings to frozen yogurt further increased total and saturated fat intake. Compared with yogurt, ice cream consumption was associated with a decreased intake of multiple micronutrients, but increased intake of sugar, total/saturated fat, cholesterol, fiber, vitamins A and E, and iron. Adding toppings to ice cream further increased sugar intake. In conclusion, frozen yogurt and ice cream were less healthy than yogurt, and adding toppings made them even less desirable. Copyright © 2017 Elsevier Inc. All rights reserved.

Use of remote video microscopy (telepathology) as an adjunct to neurosurgical frozen section consultation.

PubMed

Becker, R L; Specht, C S; Jones, R; Rueda-Pedraza, M E; O'Leary, T J

1993-08-01

We investigated the use of remote video microscopy (telepathology) to assist in the diagnosis of 52 neurosurgical frozen section cases. The TelMed system (Discovery Medical Systems, Overland Park, KS), in which the referring pathologist selects appropriate fields for transmission to the consultant, was used for the study. There was a high degree of concordance between the diagnosis rendered on the basis of transmitted video images and that rendered on the basis of direct evaluation of frozen sections; however, in seven cases there was substantial disagreement. Remote evaluation was associated with a more rapid consultation from the standpoint of the consultant, who spent approximately 2 minutes less per case when using remote microscopy; this was achieved at the expense of considerably greater effort on the part of the referring pathologist, who spent approximately 16 minutes per case selecting an average of 4.5 images for transmission to the consultant. The use of remote video microscopy for pathology consultation is associated with a complex series of tradeoffs involving cost, information loss, and timeliness of consultation.

The effective local potential method: Implementation for molecules and relation to approximate optimized effective potential techniques

NASA Astrophysics Data System (ADS)

Izmaylov, Artur F.; Staroverov, Viktor N.; Scuseria, Gustavo E.; Davidson, Ernest R.; Stoltz, Gabriel; Cancès, Eric

2007-02-01

We have recently formulated a new approach, named the effective local potential (ELP) method, for calculating local exchange-correlation potentials for orbital-dependent functionals based on minimizing the variance of the difference between a given nonlocal potential and its desired local counterpart [V. N. Staroverov et al., J. Chem. Phys. 125, 081104 (2006)]. Here we show that under a mildly simplifying assumption of frozen molecular orbitals, the equation defining the ELP has a unique analytic solution which is identical with the expression arising in the localized Hartree-Fock (LHF) and common energy denominator approximations (CEDA) to the optimized effective potential. The ELP procedure differs from the CEDA and LHF in that it yields the target potential as an expansion in auxiliary basis functions. We report extensive calculations of atomic and molecular properties using the frozen-orbital ELP method and its iterative generalization to prove that ELP results agree with the corresponding LHF and CEDA values, as they should. Finally, we make the case for extending the iterative frozen-orbital ELP method to full orbital relaxation.

Precooking as a Control for Histamine Formation during the Processing of Tuna: An Industrial Process Validation.

PubMed

Adams, Farzana; Nolte, Fred; Colton, James; De Beer, John; Weddig, Lisa

2018-02-23

An experiment to validate the precooking of tuna as a control for histamine formation was carried out at a commercial tuna factory in Fiji. Albacore tuna ( Thunnus alalunga) were brought on board long-line catcher vessels alive, immediately chilled but never frozen, and delivered to an on-shore facility within 3 to 13 days. These fish were then allowed to spoil at 25 to 30°C for 21 to 25 h to induce high levels of histamine (>50 ppm), as a simulation of "worst-case" postharvest conditions, and subsequently frozen. These spoiled fish later were thawed normally and then precooked at a commercial tuna processing facility to a target maximum core temperature of 60°C. These tuna were then held at ambient temperatures of 19 to 37°C for up to 30 h, and samples were collected every 6 h for histamine analysis. After precooking, no further histamine formation was observed for 12 to 18 h, indicating that a conservative minimum core temperature of 60°C pauses subsequent histamine formation for 12 to 18 h. Using the maximum core temperature of 60°C provided a challenge study to validate a recommended minimum core temperature of 60°C, and 12 to 18 h was sufficient to convert precooked tuna into frozen loins or canned tuna. This industrial-scale process validation study provides support at a high confidence level for the preventive histamine control associated with precooking. This study was conducted with tuna deliberately allowed to spoil to induce high concentrations of histamine and histamine-forming capacity and to fail standard organoleptic evaluations, and the critical limits for precooking were validated. Thus, these limits can be used in a hazard analysis critical control point plan in which precooking is identified as a critical control point.

Photoabsorption in sodium clusters: first principles configuration interaction calculations

NASA Astrophysics Data System (ADS)

Priya, Pradip Kumar; Rai, Deepak Kumar; Shukla, Alok

2017-05-01

We present systematic and comprehensive correlated-electron calculations of the linear photoabsorption spectra of small neutral closed- and open-shell sodium clusters (Nan, n = 2 - 6), as well as closed-shell cation clusters (Nan+, n = 3, 5). We have employed the configuration interaction (CI) methodology at the full CI (FCI) and quadruple CI (QCI) levels to compute the ground, and the low-lying excited states of the clusters. For most clusters, besides the minimum energy structures, we also consider their energetically close isomers. The photoabsorption spectra were computed under the electric-dipole approximation, employing the dipole-matrix elements connecting the ground state with the excited states of each isomer. Our calculations were tested rigorously for convergence with respect to the basis set, as well as with respect to the size of the active orbital space employed in the CI calculations. These calculations reveal that as far as electron-correlation effects are concerned, core excitations play an important role in determining the optimized ground state geometries of various clusters, thereby requiring all-electron correlated calculations. But, when it comes to low-lying optical excitations, only valence electron correlation effects play an important role, and excellent agreement with the experimental results is obtained within the frozen-core approximation. For the case of Na6, the largest cluster studied in this work, we also discuss the possibility of occurrence of plasmonic resonance in the optical absorption spectrum. Supplementary material in the form of one pdf file available from the Journal web page at http://https://doi.org/10.1140/epjd/e2017-70728-3

The frozen nucleon approximation in two-particle two-hole response functions

DOE PAGES

Ruiz Simo, I.; Amaro, J. E.; Barbaro, M. B.; ...

2017-07-10

Here, we present a fast and efficient method to compute the inclusive two-particle two-hole (2p–2h) electroweak responses in the neutrino and electron quasielastic inclusive cross sections. The method is based on two approximations. The first neglects the motion of the two initial nucleons below the Fermi momentum, which are considered to be at rest. This approximation, which is reasonable for high values of the momentum transfer, turns out also to be quite good for moderate values of the momentum transfer q ≳kF. The second approximation involves using in the “frozen” meson-exchange currents (MEC) an effective Δ-propagator averaged over the Fermimore » sea. Within the resulting “frozen nucleon approximation”, the inclusive 2p–2h responses are accurately calculated with only a one-dimensional integral over the emission angle of one of the final nucleons, thus drastically simplifying the calculation and reducing the computational time. The latter makes this method especially well-suited for implementation in Monte Carlo neutrino event generators.« less

The frozen nucleon approximation in two-particle two-hole response functions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruiz Simo, I.; Amaro, J. E.; Barbaro, M. B.

Here, we present a fast and efficient method to compute the inclusive two-particle two-hole (2p–2h) electroweak responses in the neutrino and electron quasielastic inclusive cross sections. The method is based on two approximations. The first neglects the motion of the two initial nucleons below the Fermi momentum, which are considered to be at rest. This approximation, which is reasonable for high values of the momentum transfer, turns out also to be quite good for moderate values of the momentum transfer q ≳kF. The second approximation involves using in the “frozen” meson-exchange currents (MEC) an effective Δ-propagator averaged over the Fermimore » sea. Within the resulting “frozen nucleon approximation”, the inclusive 2p–2h responses are accurately calculated with only a one-dimensional integral over the emission angle of one of the final nucleons, thus drastically simplifying the calculation and reducing the computational time. The latter makes this method especially well-suited for implementation in Monte Carlo neutrino event generators.« less

The case study of drillbit and borehole frozen water of the subglacial Lake Vostok, East Antarctica for microbial content

NASA Astrophysics Data System (ADS)

Bulat, Sergey; Doronin, Maxim; Dominique, Marie; Lipenkov, Vladimir; Lukin, Valery; Karlov, Denis; Demchenko, Leonid; Khilchenko, Margarita

The objective was to estimate microbial content and diversity in the subglacial Lake Vostok (buried beneath 4-km thick East Antarctic ice sheet) by studying the uppermost water layer which entered the borehole upon lake entry (February 5, 2012) and then shortly frozen within. The samples of so-called drillbit water frozen on a drill bit upon lake enter (RAE57) along with re-drilled so-called borehole-frozen water (RAE58) were provided for the study with the ultimate goal to discover the life in this extreme icy environment. The comprehensive analyses (constrained by Ancient DNA research criteria) of the first lake water samples - drillbit- (one sample) and borehole-frozen (3 different depths 5G-2N-3425, 3429 et 3450m), are nearly got finished. If the drillbit water sample was heavily polluted with drill fluid (at ratio 1:1), re-drilled borehole-frozen samples were proved to be rather clean but still strongly smelling kerosene and containing numerous micro-droplets of drill fluid making the ice non-transparent. The cell concentrations measured by flow cytofluorometry showed 167 cells per ml in the drillbit water sample while in borehole-frozen samples ranged from 5.5 (full-cylinder 3429m deep frozen water ice core) to 38 cells per ml (freeze-centre of 3450m deep moon-shape ice core). DNA analyses came up with total 44 bacterial phylotypes discovered by sequencing of different regions (v3-v5, v4-v8, v4-v6 et full-gene) of 16S rRNA genes. Amongst them all but two were considered to be contaminants (were present in our contaminant library, including drill fluid findings). The 1st remaining phylotype successfully passing all contamination criteria proved to be hitherto-unknown type of bacterium (group of clones, 3 allelic variants) showing less than 86% similarity with known taxa. Its phylogenetic assignment to bacterial divisions or lineages was also unsuccessful despite of the RDP has classified it belonging to OD1 uncultured Candidate Division. The 2nd phylotype was less remarkable and still dubious in terms of contamination. It was presented by just one clone and showed 93% similarity with Janthinobacterium sp of Oxalobacteraceae (Beta-Proteobacteria) - well-known ‘water-loving’ bacteria. No archaea were detected in lake water frozen samples. Thus, the unidentified and unclassified bacterial w123-10 phylotype for the first time discovered in the uppermost water layer in subglacial Lake Vostok might represent ingenious cell populations in the lake, making the life in the lake less elusive. The proof may come (as well as novel phylotype discoveries) with farther analyses (e.g., sample screening with w123-10-specific primers, 16S rRNA v4 region amplicon sequencing) of existing and newly requested moon-shape samples of borehole-frozen water which are on a way to laboratories. We are deeply grateful to Jean Robert Petit and Jean Martins, UJF-CNRS, Grenoble (France) for assistance in conducting some analyses.

Dissolved organic carbon and nitrogen release from Holocene permafrost and seasonally frozen soils

NASA Astrophysics Data System (ADS)

Wickland, K.; Waldrop, M. P.; Koch, J. C.; Jorgenson, T.; Striegl, R. G.

2017-12-01

Permafrost (perennially frozen) soils store vast amounts of carbon (C) and nitrogen (N) that are vulnerable to mobilization to the atmosphere as greenhouse gases and to terrestrial and aquatic ecosystems as dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) upon thaw. Such releases will affect the biogeochemistry of arctic and boreal regions, yet little is known about active layer (seasonally frozen) and permafrost source variability that determines DOC and TDN mobilization. We quantified DOC and TDN leachate yields from a range of active layer and permafrost soils in Alaska varying in age and C and N content to determine potential release upon thaw. Soil cores from the upper 1 meter were collected in late winter, when soils were frozen, from three locations representing a range in geographic position, landscape setting, permafrost depth, and soil types across interior Alaska. Two 15 cm-thick segments were extracted from each core: a deep active-layer horizon and a shallow permafrost horizon. Soils were thawed and leached for DOC and TDN yields, dissolved organic matter optical properties, and DOC biodegradability; soils were analyzed for C and N content, and radiocarbon content. Soils had wide-ranging C and N content (<1-44% C, <0.1-2.3% N), and varied in radiocarbon age from 450-9200 years before present - thus capturing typical ranges of boreal and arctic soils. Soil DOC and TDN yields increased linearly with soil C and N content, and decreased with increasing radiocarbon age. However, across all sites DOC and TDN yields were significantly greater from permafrost soils (0.387 ± 0.324 mg DOC g-1 soil; 0.271 ± 0.0271 mg N g-1 soil) than from active layer soils (0.210 ± 0.192 mg DOC g-1 soil; 0.00716 ± 0.00569 mg N g-1 soil). DOC biodegradability increased with increasing radiocarbon age, and was statistically similar for active layer and permafrost soils. Our findings suggest that the continuously frozen state of permafrost soils has preserved higher relative potential DOC and TDN yields compared to seasonally thawed soils exposed to annual leaching and decomposition, and that frozen soils undergo microbial processes that produce labile DOC over time.

Consistency of signal intensity and T2* in frozen ex vivo heart muscle, kidney, and liver tissue.

PubMed

Kaye, Elena A; Josan, Sonal; Lu, Aiming; Rosenberg, Jarrett; Daniel, Bruce L; Pauly, Kim Butts

2010-03-01

To investigate tissue dependence of the MRI-based thermometry in frozen tissue by quantification and comparison of signal intensity and T2* of ex vivo frozen tissue of three different types: heart muscle, kidney, and liver. Tissue samples were frozen and imaged on a 0.5 Tesla MRI scanner with ultrashort echo time (UTE) sequence. Signal intensity and T2* were determined as the temperature of the tissue samples was decreased from room temperature to approximately -40 degrees C. Statistical analysis was performed for (-20 degrees C, -5 degrees C) temperature interval. The findings of this study demonstrate that signal intensity and T2* are consistent across three types of tissue for (-20 degrees C, -5 degrees C) temperature interval. Both parameters can be used to calculate a single temperature calibration curve for all three types of tissue and potentially in the future serve as a foundation for tissue-independent MRI-based thermometry.

Treating Subvalence Correlation Effects in Domain Based Pair Natural Orbital Coupled Cluster Calculations: An Out-of-the-Box Approach.

PubMed

Bistoni, Giovanni; Riplinger, Christoph; Minenkov, Yury; Cavallo, Luigi; Auer, Alexander A; Neese, Frank

2017-07-11

The validity of the main approximations used in canonical and domain based pair natural orbital coupled cluster methods (CCSD(T) and DLPNO-CCSD(T), respectively) in standard chemical applications is discussed. In particular, we investigate the dependence of the results on the number of electrons included in the correlation treatment in frozen-core (FC) calculations and on the main threshold governing the accuracy of DLPNO all-electron (AE) calculations. Initially, scalar relativistic orbital energies for the ground state of the atoms from Li to Rn in the periodic table are calculated. An energy criterion is used for determining the orbitals that can be excluded from the correlation treatment in FC coupled cluster calculations without significant loss of accuracy. The heterolytic dissociation energy (HDE) of a series of metal compounds (LiF, NaF, AlF 3 , CaF 2 , CuF, GaF 3 , YF 3 , AgF, InF 3 , HfF 4 , and AuF) is calculated at the canonical CCSD(T) level, and the dependence of the results on the number of correlated electrons is investigated. Although for many of the studied reactions subvalence correlation effects contribute significantly to the HDE, the use of an energy criterion permits a conservative definition of the size of the core, allowing FC calculations to be performed in a black-box fashion while retaining chemical accuracy. A comparison of the CCSD and the DLPNO-CCSD methods in describing the core-core, core-valence, and valence-valence components of the correlation energy is given. It is found that more conservative thresholds must be used for electron pairs containing at least one core electron in order to achieve high accuracy in AE DLPNO-CCSD calculations relative to FC calculations. With the new settings, the DLPNO-CCSD method reproduces canonical CCSD results in both AE and FC calculations with the same accuracy.

Core flow inversion tested with numerical dynamo models

NASA Astrophysics Data System (ADS)

Rau, Steffen; Christensen, Ulrich; Jackson, Andrew; Wicht, Johannes

2000-05-01

We test inversion methods of geomagnetic secular variation data for the pattern of fluid flow near the surface of the core with synthetic data. These are taken from self-consistent 3-D models of convection-driven magnetohydrodynamic dynamos in rotating spherical shells, which generate dipole-dominated magnetic fields with an Earth-like morphology. We find that the frozen-flux approximation, which is fundamental to all inversion schemes, is satisfied to a fair degree in the models. In order to alleviate the non-uniqueness of the inversion, usually a priori conditions are imposed on the flow; for example, it is required to be purely toroidal or geostrophic. Either condition is nearly satisfied by our model flows near the outer surface. However, most of the surface velocity field lies in the nullspace of the inversion problem. Nonetheless, the a priori constraints reduce the nullspace, and by inverting the magnetic data with either one of them we recover a significant part of the flow. With the geostrophic condition the correlation coefficient between the inverted and the true velocity field can reach values of up to 0.65, depending on the choice of the damping parameter. The correlation is significant at the 95 per cent level for most spherical harmonic degrees up to l=26. However, it degrades substantially, even at long wavelengths, when we truncate the magnetic data sets to l <= 14, that is, to the resolution of core-field models. In some of the latter inversions prominent zonal currents, similar to those seen in core-flow models derived from geomagnetic data, occur in the equatorial region. However, the true flow does not contain this flow component. The results suggest that some meaningful information on the core-flow pattern can be retrieved from secular variation data, but also that the limited resolution of the magnetic core field could produce serious artefacts.

Accounting for magnetic diffusion in core flow inversions from geomagnetic secular variation

NASA Astrophysics Data System (ADS)

Amit, Hagay; Christensen, Ulrich R.

2008-12-01

We use numerical dynamos to investigate the possible role of magnetic diffusion at the top of the core. We find that the contribution of radial magnetic diffusion to the secular variation is correlated with that of tangential magnetic diffusion for a wide range of control parameters. The correlation between the two diffusive terms is interpreted in terms of the variation in the strength of poloidal flow along a columnar flow tube. The amplitude ratio of the two diffusive terms is used to estimate the probable contribution of radial magnetic diffusion to the secular variation at Earth-like conditions. We then apply a model where radial magnetic diffusion is proportional to tangential diffusion to core flow inversions of geomagnetic secular variation data. We find that including magnetic diffusion does not change dramatically the global flow but some significant local variations appear. In the non frozen-flux core flow models (termed `diffusive'), the hemispherical dichotomy between the active Atlantic and quiet Pacific is weaker, a cyclonic vortex below North America emerges and the vortex below Asia is stronger. Our results have several important geophysical implications. First, our diffusive flow models contain some flow activity at low latitudes in the Pacific, suggesting a local balance between magnetic field advection and diffusion in that region. Second, the cyclone below North America in our diffusive flows reconciles the difference between mantle-driven thermal wind predictions and frozen-flux core flow models, and is consistent with the prominent intense magnetic flux patch below North America in geomagnetic field models. Finally, we hypothesize that magnetic diffusion near the core surface plays a larger role in the geomagnetic secular variation than usually assumed.

First principles electron-correlated calculations of optical absorption in magnesium clusters★

NASA Astrophysics Data System (ADS)

Shinde, Ravindra; Shukla, Alok

2017-11-01

In this paper, we report large-scale configuration interaction (CI) calculations of linear optical absorption spectra of various isomers of magnesium clusters Mgn (n = 2-5), corresponding to valence transitions. Geometry optimization of several low-lying isomers of each cluster was carried out using coupled-cluster singles doubles (CCSD) approach, and these geometries were subsequently employed to perform ground and excited state calculations using either the full-CI (FCI) or the multi-reference singles-doubles configuration interaction (MRSDCI) approach, within the frozen-core approximation. Our calculated photoabsorption spectrum of magnesium dimer (Mg2) is in excellent agreement with the experiments both for peak positions, and intensities. Owing to the sufficiently inclusive electron-correlation effects, these results can serve as benchmarks against which future experiments, as well as calculations performed using other theoretical approaches, can be tested. Supplementary material in the form of one pdf fille available from the Journal web page at http://https://doi.org/10.1140/epjd/e2017-80356-6.

Ionic rotational branching ratios in resonant enhanced multiphoton ionization of NO via the A2Sigma(+)(3s sigma) and D2Sigma(+)(3p sigma) states

NASA Technical Reports Server (NTRS)

Rudolph, H.; Mckoy, V.; Dixit, S. N.; Huo, W. M.

1988-01-01

Results are presented for the rotationally resolved photoelectron spectra resulting from a (2 + 1) one-color resonant enhanced multiphoton ionization (REMPI) of NO via the rotationally clean S21(11.5) and mixed S11(15.5) + R21(15.5) branches of the 0-0 transition in the D-X band. The calculations were done in the fixed-nuclei frozen core approximation. The resulting photoionization spectra, convoluted with a Lorentzian detection function, agree qualitatively with experimental results of Viswanathan et al. (1986) and support their conclusion that the nonspherical nature of the molecular potential creates a substantial l-mixing in the continuum, which in turn leads to the intense Delta N = 0 peak. The rather strong photoelectron energy dependence of the rotational branching ratios of the D 2Sigma(+) S21(11.5) line was investigated and compared to the weak energy dependence of the A 2Sigma(+) R22(21.5) line.

The visible ear simulator: a public PC application for GPU-accelerated haptic 3D simulation of ear surgery based on the visible ear data.

PubMed

Sorensen, Mads Solvsten; Mosegaard, Jesper; Trier, Peter

2009-06-01

Existing virtual simulators for middle ear surgery are based on 3-dimensional (3D) models from computed tomographic or magnetic resonance imaging data in which image quality is limited by the lack of detail (maximum, approximately 50 voxels/mm3), natural color, and texture of the source material.Virtual training often requires the purchase of a program, a customized computer, and expensive peripherals dedicated exclusively to this purpose. The Visible Ear freeware library of digital images from a fresh-frozen human temporal bone was segmented, and real-time volume rendered as a 3D model of high-fidelity, true color, and great anatomic detail and realism of the surgically relevant structures. A haptic drilling model was developed for surgical interaction with the 3D model. Realistic visualization in high-fidelity (approximately 125 voxels/mm3) and true color, 2D, or optional anaglyph stereoscopic 3D was achieved on a standard Core 2 Duo personal computer with a GeForce 8,800 GTX graphics card, and surgical interaction was provided through a relatively inexpensive (approximately $2,500) Phantom Omni haptic 3D pointing device. This prototype is published for download (approximately 120 MB) as freeware at http://www.alexandra.dk/ves/index.htm.With increasing personal computer performance, future versions may include enhanced resolution (up to 8,000 voxels/mm3) and realistic interaction with deformable soft tissue components such as skin, tympanic membrane, dura, and cholesteatomas-features some of which are not possible with computed tomographic-/magnetic resonance imaging-based systems.

Third-generation site characterization: Cryogenic core collection, nuclear magnetic resonance, and electrical resistivity

NASA Astrophysics Data System (ADS)

Kiaalhosseini, Saeed

In modern contaminant hydrology, management of contaminated sites requires a holistic characterization of subsurface conditions. Delineation of contaminant distribution in all phases (i.e., aqueous, non-aqueous liquid, sorbed, and gas), as well as associated biogeochemical processes in a complex heterogeneous subsurface, is central to selecting effective remedies. Arguably, a factor contributing to the lack of success of managing contaminated sites effectively has been the limitations of site characterization methods that rely on monitoring wells and grab sediment samples. The overarching objective of this research is to advance a set of third-generation (3G) site characterization methods to overcome shortcomings of current site characterization techniques. 3G methods include 1) cryogenic core collection (C3) from unconsolidated geological subsurface to improve recovery of sediments and preserving key attributes, 2) high-throughput analysis (HTA) of frozen core in the laboratory to provide high-resolution, depth discrete data of subsurface conditions and processes, 3) resolution of non-aqueous phase liquid (NAPL) distribution within the porous media using a nuclear magnetic resonance (NMR) method, and 4) application of a complex resistivity method to track NAPL depletion in shallow geological formation over time. A series of controlled experiments were conducted to develop the C 3 tools and methods. The critical aspects of C3 are downhole circulation of liquid nitrogen via a cooling system, the strategic use of thermal insulation to focus cooling into the core, and the use of back pressure to optimize cooling. The C3 methods were applied at two contaminated sites: 1) F.E. Warren (FEW) Air Force Base near Cheyenne, WY and 2) a former refinery in the western U.S. The results indicated that the rate of core collection using the C3 methods is on the order of 30 foot/day. The C3 methods also improve core recovery and limits potential biases associated with flowing sands. HTA of frozen core was employed at the former refinery and FEW. Porosity and fluid saturations (i.e., aqueous, non-aqueous liquid, and gas) from the former refinery indicate that given in situ freezing, the results are not biased by drainage of pore fluids from the core during sample collection. At FEW, a comparison between the results of HTA of the frozen core collected in 2014 and the results of site characterization using unfrozen core, (second-generation (2G) methods) at the same locations (performed in 2010) indicate consistently higher contaminant concentrations using C 3. Many factors contribute to the higher quantification of contaminant concentrations using C3. The most significant factor is the preservation of the sediment attributes, in particular, pore fluids and volatile organic compounds (VOCs) in comparison to the unfrozen conventional sediment core. The NMR study was performed on laboratory-fabricated sediment core to resolve NAPL distribution within the porous media qualitatively and quantitatively. The fabricated core consisted of Colorado silica sand saturated with deionized water and trichloroethylene (TCE). The cores were scanned with a BRUKER small-animal scanner (2.3 Tesla, 100 MHz) at 20 °C and while the core was frozen at -25 °C. The acquired images indicated that freezing the water within the core suppressed the NMR signals of water-bound hydrogen. The hydrogen associated with TCE was still detectable since the TCE was in its liquid state (melting point of TCE is -73 °C). Therefore, qualitative detection of TCE within the sediment core was performed via the NMR scanning by freezing the water. A one-dimensional NMR scanning method was used for quantification of TCE mass distribution within the frozen core. However, the results indicated inconsistency in estimating the total TCE mass within the porous media. Downhole NMR logging was performed at the former refinery in the western U.S. to detect NAPL and to discriminate NAPL from water in the formation. The results indicated that detection of NMR signals to discriminate NAPL from water is compromised by the noise stemming from the active facilities and/or power lines passing over the site. A laboratory experiment was performed to evaluate the electrical response of unconsolidated porous media through time (30 days) while NAPL was being depleted. Sand columns (Colorado silica sand) contaminated with methyl tert-butyl ether (MTBE, a light non-aqueous phase liquid (LNAPL)) were studied. A multilevel electrode system was used to measure electrical resistivity of impacted sand by imposing alternative current. The trend of reduction in resistivity through the depth of columns over time followed depletion of LNAPL by volatilization. Finally, a field experiment was performed at the former refinery in the western U.S. to track natural losses of LNAPL over time. Multilevel systems consisting of water samplers, thermocouples, and electrodes were installed at a clean zone (background zone) and an LNAPL-impacted zone. In situ measurements of complex resistivity and temperature were taken and water sampling was performed for each depth (from 3 to 14 feet below the ground surface at one-foot spacing) within almost a year. At both locations, the results indicated decreases in apparent resistivity below the water table over time. This trend was supported by the geochemistry of the pore fluids. Overall, results indicate that application of the electrical resistivity method to track LNAPL depletion at field sites is difficult due to multiple conflicting factors affecting the geoelectrical response of LNAPL-impacted zones over time.

Commutability of NIST SRM 1955 Homocysteine and Folate in Frozen Human Serum with selected total homocysteine immunoassays and enzymatic assays.

PubMed

Nelson, Bryant C; Pfeiffer, Christine M; Zhang, Ming; Duewer, David L; Sharpless, Katherine E; Lippa, Katrice A

2008-09-01

The National Institute of Standards and Technology (NIST) has recently developed Standard Reference Material (SRM) 1955 Homocysteine and Folate in Frozen Human Serum with certified values for total homocysteine (tHcy) and 5-methyl-tetrahydrofolic acid. NIST has performed an international, interlaboratory assessment of SRM 1955 commutability; results are reported for tHcy only. Total Hcy was measured in 20 patient sera and in 3 levels of SRM 1955 using 14 immunoassays and/or enzymatic assays. Liquid chromatography/tandem mass spectrometry was utilized as the reference assay. An "errors-in-variables" statistical model was utilized to assess the commutability of SRM 1955. Normalized residuals ranged from -2.65 to 2.19 for SRM 1955. The median interlaboratory/interassay imprecision (CV) was approximately 4% for patient specimens and ranged from approximately 3% to approximately 7% for SRM 1955. The median intra-assay imprecision ranged from approximately 1% to approximately 13%. Orthogonal residuals, as a descriptor of assay accuracy, ranged from 0.29 to 7.71 and from 0.20 to 2.22 for patient specimens and SRM 1955 samples, respectively. The current study suggests that SRM 1955 is commutable with the investigated tHcy assays; however, a broader specimen set needs to be evaluated to completely substantiate this conclusion.

The use of frozen plasma samples in thromboelastometry.

PubMed

Schoergenhofer, Christian; Buchtele, Nina; Schwameis, Michael; Bartko, Johann; Jilma, Bernd; Jilma-Stohlawetz, Petra

2017-11-01

Thromboelastometry is increasingly used in the clinical and scientific setting. The use of frozen plasma samples may be useful in overcoming certain limitations such as local and timely availability. Whole blood (WB) samples of 20 healthy volunteers were obtained, and plasma was generated. NATEM (n = 20), EXTEM (n = 20) and INTEM (n = 8) analyses were performed in WB, fresh plasma and frozen and thawed plasma. Dabigatran (500, 1000 ng/ml), rivaroxaban (100, 200 ng/ml) or alteplase (333 ng/ml) were added ex vivo to WB, and thromboelastometry was performed in WB and in frozen and thawed plasma samples. Clot formation time, mean clot firmness and the area under the curve were significantly altered in plasma compared to WB. In INTEM and EXTEM analysis, clotting time (CT) was comparable between WB (100%) and fresh (INTEM 114% and EXTEM 93%, ratio of the means) and frozen plasma samples (85 and 99%), whereas in NATEM analysis, the CT increased in fresh (193%) and frozen plasma samples (130%). Dabigatran dose-dependently increased the CT approximately 5- and 9-fold in WB and even more pronounced 10- and 26-fold in plasma. Accordingly, rivaroxaban dose-dependently increased the CT 2- and 2.7-fold in WB, and 3.5- and 4-fold in plasma samples. Hyperfibrinolysis was achieved by addition of alteplase in all WB samples and was reproducible in plasma samples. In conclusion, thromboelastometry, especially INTEM and EXTEM analyses, is possible using frozen and stored plasma samples with comparable results to the corresponding whole blood samples.

Closed-form analytical solutions of high-temperature heat pipe startup and frozen startup limitation

NASA Technical Reports Server (NTRS)

Cao, Y.; Faghri, A.

1992-01-01

Previous numerical and experimental studies indicate that the high-temperature heat pipe startup process is characterized by a moving hot zone with relatively sharp fronts. Based on the above observation, a flat-front model for an approximate analytical solution is proposed. A closed-form solution related to the temperature distribution in the hot zone and the hot zone length as a function of time are obtained. The analytical results agree well with the corresponding experimental data, and provide a quick prediction method for the heat pipe startup performance. Finally, a heat pipe limitation related to the frozen startup process is identified, and an explicit criterion for the high-temperature heat pipe startup is derived. The frozen startup limit identified in this paper provides a fundamental guidance for high-temperature heat pipe design.

Communication: Biological applications of coupled-cluster frozen-density embedding

NASA Astrophysics Data System (ADS)

Heuser, Johannes; Höfener, Sebastian

2018-04-01

We report the implementation of the Laplace-transform scaled opposite-spin (LT-SOS) resolution-of-the-identity second-order approximate coupled-cluster singles and doubles (RICC2) combined with frozen-density embedding for excitation energies and molecular properties. In the present work, we furthermore employ the Hartree-Fock density for the interaction energy leading to a simplified Lagrangian which is linear in the Lagrangian multipliers. This approximation has the key advantage of a decoupling of the coupled-cluster amplitude and multipliers, leading also to a significant reduction in computation time. Using the new simplified Lagrangian in combination with efficient wavefunction models such as RICC2 or LT-SOS-RICC2 and density-functional theory (DFT) for the environment molecules (CC2-in-DFT) enables the efficient study of biological applications such as the rhodopsin and visual cone pigments using ab initio methods as routine applications.

Planetary Drilling and Resources at the Moon and Mars

NASA Technical Reports Server (NTRS)

George, Jeffrey A.

2012-01-01

Drilling on the Moon and Mars is an important capability for both scientific and resource exploration. The unique requirements of spaceflight and planetary environments drive drills to different design approaches than established terrestrial technologies. A partnership between NASA and Baker Hughes Inc. developed a novel approach for a dry rotary coring wireline drill capable of acquiring continuous core samples at multi-meter depths for low power and mass. The 8.5 kg Bottom Hole Assembly operated at 100 We and without need for traditional drilling mud or pipe. The technology was field tested in the Canadian Arctic in sandstone, ice and frozen gumbo. Planetary resources could play an important role in future space exploration. Lunar regolith contains oxygen and metals, and water ice has recently been confirmed in a shadowed crater at the Moon.s south pole. Mars possesses a CO2 atmosphere, frozen water ice at the poles, and indications of subsurface aquifers. Such resources could provide water, oxygen and propellants that could greatly simplify the cost and complexity of exploration and survival. NASA/JSC/EP/JAG

DARE Mission Design: Low RFI Observations from a Low-Altitude Frozen Lunar Orbit

NASA Technical Reports Server (NTRS)

Plice, Laura; Galal, Ken; Burns, Jack O.

2017-01-01

The Dark Ages Radio Explorer (DARE) seeks to study the cosmic Dark Ages approximately 80 to 420 million years after the Big Bang. Observations require truly quiet radio conditions, shielded from Sun and Earth electromagnetic (EM) emissions, on the far side of the Moon. DAREs science orbit is a frozen orbit with respect to lunar gravitational perturbations. The altitude and orientation of the orbit remain nearly fixed indefinitely, maximizing science time without the need for maintenance. DAREs observation targets avoid the galactic center and enable investigation of the universes first stars and galaxies.

Geomagnetic field models incorporating physical constraints on the secular variation

NASA Technical Reports Server (NTRS)

Constable, Catherine; Parker, Robert L.

1993-01-01

This proposal has been concerned with methods for constructing geomagnetic field models that incorporate physical constraints on the secular variation. The principle goal that has been accomplished is the development of flexible algorithms designed to test whether the frozen flux approximation is adequate to describe the available geomagnetic data and their secular variation throughout this century. These have been applied to geomagnetic data from both the early and middle part of this century and convincingly demonstrate that there is no need to invoke violations of the frozen flux hypothesis in order to satisfy the available geomagnetic data.

Surface Deformation Caused by Pressure Changes in the Fluid Core

NASA Technical Reports Server (NTRS)

Fang, Ming; Hager, Bradford H.; Herring, Thomas A.

1995-01-01

Pressure load Love numbers are presented for the mantle deformation induced by the variation of the pressure field at the core mantle boundary (CNB). We find that the CMB geostrophic pressure fields, derived from 'frozen-flux' core surface flow estimates at epochs 1965 and 1975, produce a relative radial velocity (RRV) field in the range of 3mm/decade with uplift near the equator and subsidence near the poles. The contribution of this mechanism to the change in the length of day (l.o.d) is small --- about 2.3 x 10(exp -2) ms/decade. The contribution to the time variation of the ellipticity coefficient is more important --- -1.3 x 10(exp -11)/yr.

Zonation and assessment of frozen-ground conditions along the China-Russia Crude Oil Pipeline route from Mo’he to Daqing, Northeastern China

NASA Astrophysics Data System (ADS)

Jin, H.; Hao, J.; Chang, X.

2009-12-01

The proposed China-Russia Crude Oil Pipeline (CRCOP), 813 mm in diameter, is designed to transport 603,000 barrels of Siberian crude oil per day using conventional burial across 1,030 km of frozen-ground. About 500 boreholes, with depths of 5 to 20 m, were drilled and cored for analyses, and the frozen-ground conditions were evaluated. After detailed surveys and analyses of the permafrost conditions along the pipeline route, a conventional burial construction mode at a nominal depth of 1.5 m was adopted. This paper discusses the principles and criteria for the zonation and assessment of the frozen-ground environments and conditions of engineering geology for the design, construction, operation of the pipeline system based on an extensive and in-depth summary and analysis of the survey and exploration data. Full consideration of the characteristics of pipelining crude oil at ambient temperatures in the permafrost regions and the interactive processes between the pipeline and foundation soils were taken into account. Two zones of frozen-ground environment and conditions of engineering geology, i. e. seasonally-frozen-ground and permafrost, were defined on the basis of the regional distribution and differentiations in frozen-ground environments and conditions. Then, four subzones of the permafrost zone were classified according to the areal extent, taking into consideration the temperatures and thicknesses of permafrost, as well as changes in vegetation coverage. In the four subzones, 151 sections of engineering geology were categorized according to the ice/moisture contents of the permafrost, as well as the classes of frost-heaving and thaw-settlement potentials. These 151 sections are comprehensively summarized into four types for engineering construction and operation: good, fair, poor, and very poor, for overall conditions of engineering geology. The zonation, assessment principles and criteria have been applied in the design of the pipeline. They have also been used as the scientific bases for the construction, environmental management, operation and maintenance/contingency plans

The Nonisothermal Stage of Magnetic Star Formation. I. Formulation of the Problem and Method of Solution

NASA Astrophysics Data System (ADS)

Kunz, Matthew W.; Mouschovias, Telemachos Ch.

2009-03-01

We formulate the problem of the formation and subsequent evolution of fragments (or cores) in magnetically supported, self-gravitating molecular clouds in two spatial dimensions. The six-fluid (neutrals, electrons, molecular and atomic ions, positively charged, negatively charged, and neutral grains) physical system is governed by the radiation, nonideal magnetohydrodynamic equations. The magnetic flux is not assumed to be frozen in any of the charged species. Its evolution is determined by a newly derived generalized Ohm's law, which accounts for the contributions of both elastic and inelastic collisions to ambipolar diffusion and Ohmic dissipation. The species abundances are calculated using an extensive chemical-equilibrium network. Both MRN and uniform grain size distributions are considered. The thermal evolution of the protostellar core and its effect on the dynamics are followed by employing the gray flux-limited diffusion approximation. Realistic temperature-dependent grain opacities are used that account for a variety of grain compositions. We have augmented the publicly available Zeus-MP code to take into consideration all these effects and have modified several of its algorithms to improve convergence, accuracy, and efficiency. Results of magnetic star formation simulations that accurately track the evolution of a protostellar fragment from a density sime103 cm-3 to a density sime1015 cm-3, while rigorously accounting for both nonideal MHD processes and radiative transfer, are presented in a separate paper.

Effect of Novel Quick Freezing Techniques Combined with Different Thawing Processes on Beef Quality

PubMed Central

Yoo, Seon-Mi; Han, Gui-Jung

2014-01-01

This study investigated the effect of various freezing and thawing techniques on the quality of beef. Meat samples were frozen using natural convection freezing (NF), individual quick freezing (IQF), or cryogenic freezing (CF) techniques, followed by natural convection thawing (NCT) or running water thawing (RT). The meat was frozen until the core temperature reached -12℃ and then stored at -24℃, followed by thawing until the temperature reached 5℃. Quality parameters, such as the pH, water binding properties, CIE color, shear force, and microstructure of the beef were elucidated. Although the freezing and thawing combinations did not cause remarkable changes in the quality parameters, rapid freezing, in the order of CF, IQF, and NF, was found to minimize the quality deterioration. In the case of thawing methods, NCT was better than RT and the meat quality was influence on the thawing temperature rather than the thawing rate. Although the microstructure of the frozen beef exhibited an excessive loss of integrity after the freezing and thawing, it did not cause any remarkable change in the beef quality. Taken together, these results demonstrate that CF and NCT form the best combination for beef processing; however, IQF and NCT may have practical applications in the frozen food industry. PMID:26761674

Target with a frozen nuclear polarization for experiments at low energies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Borisov, N.S.; Matafonov, V.N.; Neganov, A.B.

1995-09-01

The short history of the development of frozen spin polarized targets at the Laboratory of Nuclear Problems JINR is given. The latest development is the target with a frozen spin polarization of protons in 1,2- propanediol with a paramagnetic Cr{sup {ital V}} impurity, intended for polarization parameter studies in np-scattering at approximately 15 MeV neutron energy. The target of cylindrical shape of 2 cm diameter and 6 cm long with an initial polarization of 95{plus_minus}3{percent} obtainable by the dynamic polarization technique is placed at a temperature about 20 mK in a magnetic field of 0.37 T generated by a magneticmore » system, which provides a large aperture for scattered particles. The relaxation time for the spin polarization is about 1000 hours. {copyright} {ital 1995 American Institute of Physics.}« less

Thermodynamic properties of arsenic compounds and the heat of formation of the As atom from high level electronic structure calculations.

PubMed

Feller, David; Vasiliu, Monica; Grant, Daniel J; Dixon, David A

2011-12-29

Structures, vibrational frequencies, atomization energies at 0 K, and heats of formation at 0 and 298 K are predicted for the compounds As(2), AsH, AsH(2), AsH(3), AsF, AsF(2), and AsF(3) from frozen core coupled cluster theory calculations performed with large correlation consistent basis sets, up through augmented sextuple zeta quality. The coupled cluster calculations involved up through quadruple excitations. For As(2) and the hydrides, it was also possible to examine the impact of full configuration interaction on some of the properties. In addition, adjustments were incorporated to account for extrapolation to the frozen core complete basis set limit, core/valence correlation, scalar relativistic effects, the diagonal Born-Oppenheimer correction, and atomic spin orbit corrections. Based on our best theoretical D(0)(As(2)) and the experimental heat of formation of As(2), we propose a revised 0 K arsenic atomic heat of formation of 68.86 ± 0.8 kcal/mol. While generally good agreement was found between theory and experiment, the heat of formation of AsF(3) was an exception. Our best estimate is more than 7 kcal/mol more negative than the single available experimental value, which argues for a re-examination of that measurement. © 2011 American Chemical Society

Short communication: The effects of frozen storage on the survival of probiotic microorganisms found in traditionally and commercially manufactured kefir.

PubMed

O'Brien, K V; Aryana, K J; Prinyawiwatkul, W; Ordonez, K M Carabante; Boeneke, C A

2016-09-01

Kefir is a fermented milk traditionally made from a unique starter culture, which consists of numerous bacteria and yeast species bound together in an exopolysaccharide matrix produced by certain lactic acid bacteria. Many health benefits are associated with traditionally produced kefir; however, bulging and leaking packaging, caused by secondary yeast fermentation during storage, has limited large-scale manufacture. Commercial kefir products have been designed to reduce these effects by using a pure starter culture consisting of a mixture of bacteria and yeast species that give a flavor similar to traditional kefir, but some health benefits may be lost in commercial production due to reduced microbial diversity and lack of beneficial exopolysaccharides. In this study, traditional and commercial kefir was frozen to study the effects of frozen storage on the viability of probiotic bacteria over time. Traditional kefir was prepared by inoculating 1L of pasteurized whole goat milk with approximately 30g of kefir grains. Commercial kefir was prepared by inoculating 1L of full-fat, pasteurized goat milk with a commercial kefir starter. The milk was allowed to ferment at room temperature (24-28°C) until pH 4.6 was reached. Samples were frozen (-8 to -14°C) immediately following the completion of fermentation and were thawed and plated for lactobacilli, lactococci, and yeasts on d 0, 7, 14, and 30 of frozen storage. Lactobacilli, lactococci, and yeasts were significantly reduced in number during frozen storage; however, the traditionally produced kefir was shown to have significantly higher counts of bacteria and yeast at each sampling. We concluded that frozen storage and the development of frozen kefir products could eliminate most packaging concerns associated with the large-scale manufacture of traditionally produced kefir, resulting in increased production and marketability of this healthful product. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Every Day Lots of Ways. An Interdisciplinary Nutrition Curriculum for Kindergarten-Sixth Grade.

ERIC Educational Resources Information Center

Bagby, Robin; Campbell, V. Star; Achterberg, Cheryl; Probart, Claudia; Ebel, Kimberlyn

This K-6 interdisciplinary nutrition curriculum focuses on vegetables and fruits. The lessons use core subjects to promote consumption of fresh, frozen, and canned vegetables and fruits; vegetable and fruit juices; and dried fruits. The lessons also investigate the Food Guide Pyramid. Each lesson plan has a section entitled Extensions. The…

Presence of Nitrate-Accumulating Sulfur Bacteria and Their Influence on Nitrogen Cycling in a Shallow Coastal Marine Sediment

PubMed Central

Sayama, Mikio

2001-01-01

Nitrate flux between sediment and water, nitrate concentration profile at the sediment-water interface, and in situ sediment denitrification activity were measured seasonally at the innermost part of Tokyo Bay, Japan. For the determination of sediment nitrate concentration, undisturbed sediment cores were sectioned into 5-mm depth intervals and each segment was stored frozen at −30°C. The nitrate concentration was determined for the supernatants after centrifuging the frozen and thawed sediments. Nitrate in the uppermost sediment showed a remarkable seasonal change, and its seasonal maximum of up to 400 μM was found in October. The directions of the diffusive nitrate fluxes predicted from the interfacial concentration gradients were out of the sediment throughout the year. In contrast, the directions of the total nitrate fluxes measured by the whole-core incubation were into the sediment at all seasons. This contradiction between directions indicates that a large part of the nitrate pool extracted from the frozen surface sediments is not a pore water constituent, and preliminary examinations demonstrated that the nitrate was contained in the intracellular vacuoles of filamentous sulfur bacteria dwelling on or in the surface sediment. Based on the comparison between in situ sediment denitrification activity and total nitrate flux, it is suggested that intracellular nitrate cannot be directly utilized by sediment denitrification, and the probable fate of the intracellular nitrate is hypothesized to be dissimilatory reduction to ammonium. The presence of nitrate-accumulating sulfur bacteria therefore may lower nature's self-purification capacity (denitrification) and exacerbate eutrophication in shallow coastal marine environments. PMID:11472923

Monitoring recharge in areas of seasonally frozen ground in the Columbia Plateau and Snake River Plain, Idaho, Oregon, and Washington

USGS Publications Warehouse

Mastin, Mark; Josberger, Edward

2014-01-01

Seasonally frozen ground occurs over approximately one‑third of the contiguous United States, causing increased winter runoff. Frozen ground generally rejects potential groundwater recharge. Nearly all recharge from precipitation in semi-arid regions such as the Columbia Plateau and the Snake River Plain in Idaho, Oregon, and Washington, occurs between October and March, when precipitation is most abundant and seasonally frozen ground is commonplace. The temporal and spatial distribution of frozen ground is expected to change as the climate warms. It is difficult to predict the distribution of frozen ground, however, because of the complex ways ground freezes and the way that snow cover thermally insulates soil, by keeping it frozen longer than it would be if it was not snow covered or, more commonly, keeping the soil thawed during freezing weather. A combination of satellite remote sensing and ground truth measurements was used with some success to investigate seasonally frozen ground at local to regional scales. The frozen-ground/snow-cover algorithm from the National Snow and Ice Data Center, combined with the 21-year record of passive microwave observations from the Special Sensor Microwave Imager onboard a Defense Meteorological Satellite Program satellite, provided a unique time series of frozen ground. Periodically repeating this methodology and analyzing for trends can be a means to monitor possible regional changes to frozen ground that could occur with a warming climate. The Precipitation-Runoff Modeling System watershed model constructed for the upper Crab Creek Basin in the Columbia Plateau and Reynolds Creek basin on the eastern side of the Snake River Plain simulated recharge and frozen ground for several future climate scenarios. Frozen ground was simulated with the Continuous Frozen Ground Index, which is influenced by air temperature and snow cover. Model simulation results showed a decreased occurrence of frozen ground that coincided with increased temperatures in the future climate scenarios. Snow cover decreased in the future climate scenarios coincident with the temperature increases. Although annual precipitation was greater in future climate scenarios, thereby increasing the amount of water available for recharge over current (baseline) simulations, actual evapotranspiration also increased and reduced the amount of water available for recharge over baseline simulations. The upper Crab Creek model shows no significant trend in the rates of recharge in future scenarios. In these scenarios, annual precipitation is greater than the baseline averages, offsetting the effects of greater evapotranspiration in future scenarios. In the Reynolds Creek Basin simulations, precipitation was held constant in future scenarios and recharge was reduced by 1.0 percent for simulations representing average conditions in 2040 and reduced by 4.3 percent for simulations representing average conditions in 2080. The focus of the results of future scenarios for the Reynolds Creek Basin was the spatial components of selected hydrologic variables for this 92 square mile mountainous basin with 3,600 feet of relief. Simulation results from the watershed model using the Continuous Frozen Ground Index provided a relative measure of change in frozen ground, but could not identify the within-soil processes that allow or reject available water to recharge aquifers. The model provided a means to estimate what might occur in the future under prescribed climate scenarios, but more detailed energy-balance models of frozen-ground hydrology are needed to accurately simulate recharge under seasonally frozen ground and provide a better understanding of how changes in climate may alter infiltration.

Frozen flux violation, electron demagnetization and magnetic reconnection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scudder, J. D.; Karimabadi, H.; Roytershteyn, V.

2015-10-15

We argue that the analogue in collisionless plasma of the collisional diffusion region of magnetic reconnection is properly defined in terms of the demagnetization of the plasma electrons that enable “frozen flux” slippage to occur. This condition differs from the violation of the “frozen-in” condition, which only implies that two fluid effects are involved, rather than the necessary slippage of magnetic flux as viewed in the electron frame. Using 2D Particle In Cell (PIC) simulations, this approach properly finds the saddle point region of the flux function. Our demagnetization conditions are the dimensionless guiding center approximation expansion parameters for electronsmore » which we show are observable and determined locally by the ratio of non-ideal electric to magnetic field strengths. Proxies for frozen flux slippage are developed that (a) are measurable on a single spacecraft, (b) are dimensionless with theoretically justified threshold values of significance, and (c) are shown in 2D simulations to recover distinctions theoretically possible with the (unmeasurable) flux function. A new potentially observable dimensionless frozen flux rate, Λ{sub Φ}, differentiates significant from anecdotal frozen flux slippage. A single spacecraft observable, ϒ, is shown with PIC simulations to be essentially proportional to the unobservable local Maxwell frozen flux rate. This relationship theoretically establishes electron demagnetization in 3D as the general cause of frozen flux slippage. In simple 2D cases with an isolated central diffusion region surrounded by separatrices, these diagnostics uniquely identify the traditional diffusion region (without confusing it with the two fluid “ion-diffusion” region) and clarify the role of the separatrices where frozen flux violations do occur but are not substantial. In the more complicated guide and asymmetric 2D cases, substantial flux slippage regions extend out along, but inside of, the preferred separatrices, demonstrating that Λ{sub Φ} ≠ 0 violations are present over significant distances (in ion inertial units) from the separator identified by the 2D flux function; these violations are, however, generally weaker than seen at known separators in 2D simulations.« less

ELECTROSTATIC BARRIER AGAINST DUST GROWTH IN PROTOPLANETARY DISKS. II. MEASURING THE SIZE OF THE 'FROZEN' ZONE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Okuzumi, Satoshi; Sakagami, Masa-aki; Tanaka, Hidekazu

2011-04-20

Coagulation of submicron-sized dust grains into porous aggregates is the initial step of dust evolution in protoplanetary disks. Recently, it has been pointed out that negative charging of dust in the weakly ionized disks could significantly slow down the coagulation process. In this paper, we apply the growth criteria obtained in Paper I to finding out a location ('frozen' zone) where the charging stalls dust growth at the fractal growth stage. For low-turbulence disks, we find that the frozen zone can cover a major part of the disks at a few to 100 AU from the central star. The maximummore » mass of the aggregates is approximately 10{sup -7}g at 1 AU and as small as a few monomer masses at 100 AU. Strong turbulence can significantly reduce the size of the frozen zone, but such turbulence will cause the fragmentation of macroscopic aggregates at later stages. We examine a possibility that complete freezeout of dust evolution in low-turbulence disks could be prevented by global transport of dust in the disks. Our simple estimation shows that global dust transport can lead to the supply of macroscopic aggregates and the removal of frozen aggregates on a timescale of 10{sup 6} yr. This overturns the usual understanding that tiny dust particles get depleted on much shorter timescales unless collisional fragmentation is effective. The frozen zone together with global dust transport might explain 'slow' ({approx}10{sup 6} yr) dust evolution suggested by infrared observation of T Tauri stars and by radioactive dating of chondrites.« less

Frozen orbit realization using LQR analogy

NASA Astrophysics Data System (ADS)

Nagarajan, N.; Rayan, H. Reno

In the case of remote sensing orbits, the Frozen Orbit concept minimizes altitude variations over a given region using passive means. This is achieved by establishing the mean eccentricity vector at the orbital poles i.e., by fixing the mean argument of perigee at 90 deg with an appropriate eccentricity to balance the perturbations due to zonal harmonics J2 and J3 of the Earth's potential. Eccentricity vector is a vector whose magnitude is the eccentricity and direction is the argument of perigee. The launcher dispersions result in an eccentricity vector which is away from the frozen orbit values. The objective is then to formulate an orbit maneuver strategy to optimize the fuel required to achieve the frozen orbit in the presence of visibility and impulse constraints. It is shown that the motion of the eccentricity vector around the frozen perigee can be approximated as a circle. Combining the circular motion of the eccentricity vector around the frozen point and the maneuver equation, the following discrete equation is obtained. X(k+1) = AX(k) + Bu(k), where X is the state (i.e. eccentricity vector components), A the state transition matrix, u the scalar control force (i.e. dV in this case) and B the control matrix which transforms dV into eccentricity vector change. Based on this, it is shown that the problem of optimizing the fuel can be treated as a Linear Quadratic Regulator (LQR) problem in which the maneuver can be solved by using control system design tools like MATLAB by deriving an analogy LQR design.

Characterization and depositional and evolutionary history of the Apollo 17 deep drill core

NASA Technical Reports Server (NTRS)

Morris, R. V.; Lauer, H. V., Jr.; Gose, W. A.

1979-01-01

With a depth resolution of about 0.5 cm, the stratigraphy of the approximately 3 m Apollo 17 deep drill core by measurement of the total FeO concentration is characterized along with the FMR surface exposure (maturity) index Is/FeO, the metallic iron concentration Fe-vsm, and the FMR linewidth delta-H. For stratigraphic characterization, the first two parameters are the most important. Most of the core is characterized by a FeO concentration of approximately 15.5 wt. %; there is a more mafic zone in the upper approximately 75 cm where the maximum FeO concentration is approximately 18.5 wt. %, and a more felsic zone between approximately 225 and 260 cm where the minimum FeO concentration is approximately 14.0%. As indicated by Is/FeO, most of the soil in the core is submature to mature; the only immature zone is located between approximately 20 and 60 cm and is one of the most distinctive features in the core. A two stage model for the depositional and evolutionary history of the Apollo 17 deep drill core is proposed: (1) deposition by one event approximately 110 m.y. ago or deposition by a sequence of closely spaced events initating a maximum of approximately 200 m.y. ago and terminating approximately 110 m.y. ago, (2) in situ reworking (gardening) to a depth of approximately 26 cm in the period between approximately 110 m.y. ago and the present day.

Heisenberg operator approach for spin squeezing dynamics

NASA Astrophysics Data System (ADS)

Bhattacherjee, Aranya Bhuti; Sharma, Deepti; Pelster, Axel

2017-12-01

We reconsider the one-axis twisting Hamiltonian, which is commonly used for generating spin squeezing, and treat its dynamics within the Heisenberg operator approach. To this end we solve the underlying Heisenberg equations of motion perturbatively and evaluate the expectation values of the resulting time-dependent Heisenberg operators in order to determine approximately the dynamics of spin squeezing. Comparing our results with those originating from exact numerics reveals that they are more accurate than the commonly used frozen spin approximation.

Near-Surface Profiles of Water Stable Isotope Components and Indicated Transitional History of Ice-Wedge Polygons Near Barrow

NASA Astrophysics Data System (ADS)

Iwahana, G.; Wilson, C.; Newman, B. D.; Heikoop, J. M.; Busey, R.

2017-12-01

Wetlands associated with ice-wedge polygons are commonly distributed across the Arctic Coastal Plain of northern Alaska, a region underlain by continuous permafrost. Micro-topography of the ice-wedge polygons controls local hydrology, and the micro-topography could be altered due to factors such like surface vegetation, wetness, freeze-thaw cycles, and permafrost degradation/aggradation under climate change. Understanding status of the wetlands in the near future is important because it determines biogeochemical cycle, which drives release of greenhouse gases from the ground. However, transitional regime of the ice-wedge polygons under the changing climate is not fully understood. In this study, we analyzed geochemistry of water extracted from frozen soil cores sampled down to about 1m depth in 2014 March at NGEE-Arctic sites in the Barrow Environmental Observatory. The cores were sampled from troughs/rims/centers of five different low-centered or flat-centered polygons. The frozen cores are divided into 5-10cm cores for each location, thawed in sealed plastic bags, and then extracted water was stored in vials. Comparison between the profiles of geochemistry indicated connection of soil water in the active layer at different location in a polygon, while it revealed that distinctly different water has been stored in permafrost layer at troughs/rims/centers of some polygons. Profiles of volumetric water content (VWC) showed clear signals of freeze-up desiccation in the middle of saturated active layers as low VWC anomalies at most sampling points. Water in the active layer and near-surface permafrost was classified into four categories: ice wedge / fresh meteoric / transitional / highly fractionated water. The overall results suggested prolonged separation of water in the active layer at the center of low-centered polygons without lateral connection in water path in the past.

Alternative definitions of the frozen energy in energy decomposition analysis of density functional theory calculations.

PubMed

Horn, Paul R; Head-Gordon, Martin

2016-02-28

In energy decomposition analysis (EDA) of intermolecular interactions calculated via density functional theory, the initial supersystem wavefunction defines the so-called "frozen energy" including contributions such as permanent electrostatics, steric repulsions, and dispersion. This work explores the consequences of the choices that must be made to define the frozen energy. The critical choice is whether the energy should be minimized subject to the constraint of fixed density. Numerical results for Ne2, (H2O)2, BH3-NH3, and ethane dissociation show that there can be a large energy lowering associated with constant density orbital relaxation. By far the most important contribution is constant density inter-fragment relaxation, corresponding to charge transfer (CT). This is unwanted in an EDA that attempts to separate CT effects, but it may be useful in other contexts such as force field development. An algorithm is presented for minimizing single determinant energies at constant density both with and without CT by employing a penalty function that approximately enforces the density constraint.

Effect of ELISA kit manufacturing process and incubation time on progesterone concentration measured in dog serum for ovulation diagnosis - Short communication.

PubMed

Thuróczy, Julianna; Reiczigel, Jenő; Balogh, Lajos

2016-09-01

Twenty-two serum samples of healthy bitches were tested with the frozen and lyophilised version of the same ELISA kit (Quanticheck, Faculty of Veterinary Science, Budapest, Hungary). Samples were chosen on the basis of their progesterone (P4) concentrations, which were between 1.00 and 20.00 ng/mL. As it is well known, this range has the highest clinical relevance in ovulation diagnosis. Both types of microplates were read at 15-min intervals from the 15th until the 90th minute (min) of incubation, and the results were compared with those of frozen plates at 60 min of incubation as 100 percent. Lyophilised microplates gave on average 18 percent higher results than the frozen version at equal incubation times. The highest difference between lyophilised and frozen samples was observed at 45 and 60 min of incubation. Ninety-four percent of the reaction in the frozen microplate occurred in the first 15 min, and during the subsequent 30 min the reaction seemingly stopped. After the 45th min of incubation, this 94 percent increased to 108 percent in the subsequent 30 min, which remained the final approximate result at the end of the 90 min of incubation. In contrast to the frozen microplate, the measured concentration increased continuously in the lyophilised version and reached the highest level at the 60th min. The results of the lyophilised microplate reached the same level at 30 min of incubation as those of the frozen version at 60 min. In conclusion, a mechanical increase or decrease of the incubation time does not generate a linear change in the test results. This study demonstrated that the results of a series of samples collected from the same bitch cannot be compared if they are measured with different laboratory methods or different ELISA kits.

Implications of the pH and temperature of diluted, cooled boar semen on fresh and frozen-thawed sperm motility characteristics.

PubMed

Purdy, P H; Tharp, N; Stewart, T; Spiller, S F; Blackburn, H D

2010-10-15

Boar semen is typically collected, diluted and cooled for AI use over numerous days, or frozen immediately after shipping to capable laboratories. The storage temperature and pH of the diluted, cooled boar semen could influence the fertility of boar sperm. Therefore, the purpose of this study was to determine the effects of pH and storage temperature on fresh and frozen-thawed boar sperm motility end points. Semen samples (n = 199) were collected, diluted, cooled and shipped overnight to the National Animal Germplasm Program laboratory for freezing and analysis from four boar stud facilities. The temperature, pH and motility characteristics, determined using computer automated semen analysis, were measured at arrival. Samples were then cryopreserved and post-thaw motility determined. The commercial stud was a significant source of variation for mean semen temperature and pH, as well as total and progressive motility, and numerous other sperm motility characteristics. Based on multiple regression analysis, pH was not a significant source of variation for fresh or frozen-thawed boar sperm motility end points. However, significant models were derived which demonstrated that storage temperature, boar, and the commercial stud influenced sperm motility end points and the potential success for surviving cryopreservation. We inferred that maintaining cooled boar semen at approximately 16 °C during storage will result in higher fresh and frozen-thawed boar sperm quality, which should result in greater fertility. Copyright © 2010 Elsevier Inc. All rights reserved.

Soil Incubation Study to Assess the Impacts of Manure Application and Climate Change on Greenhouse Gas Emissions from Agricultural Soil

NASA Astrophysics Data System (ADS)

Schiavone, K.; Barbieri, L.; Adair, C.

2015-12-01

Agricultural fields in Vermont's Lake Champlain Basin have problems with the loss of nutrients due to runoff which creates eutrophic conditions in the lakes, ponds and rivers. In efforts to retain nitrogen and other nutrients in the soil farmers have started to inject manure rather than spraying it. Our understanding of the effects this might have on the volatilization of nitrogen into nitrous oxide is limited. Already, agriculture produces 69% of the total nitrous oxide emissions in the US. Understanding that climate change will affect the future of agriculture in Vermont, we set up a soil core incubation test to monitor the emissions of CO₂ and N₂O using a Photoacoustic Gas Sensor (PAS). Four 10 cm soil cores were taken from nine different fertilizer management plots in a No Till corn field; Three Injected plots, three Broadcast plots, and three Plow plots. Frozen soil cores were extracted in early April, and remained frozen before beginning the incubation experiment to most closely emulate three potential spring environmental conditions. The headspace was monitored over one week to get emission rates. This study shows that environmental and fertilizer treatments together do not have a direct correlation to the amount of CO₂ and N₂O emissions from agricultural soil. However, production of CO₂ was 26% more in warmer environmental conditions than in variable(freeze/thaw) environmental conditions. The injected fertilizer produced the most emissions, both CO₂ and N₂O. The total N₂O emissions from Injected soil cores were 2.2x more than from traditional broadcast manure cores. We believe this is likely due to the addition of rich organic matter under anaerobic soil conditions. Although, injected fertilizer is a better application method for reducing nutrient runoff, the global warming potential of N₂O is 298 times that of CO₂. With climate change imminent, assessing the harmful effects and benefits of injected fertilizer is a crucial next step in agricultural management.

GAMMA IRRADIATION OF SALMONELLA SPECIES IN FROZEN WHOLE EGG

DOE Office of Scientific and Technical Information (OSTI.GOV)

Comer, A.G.; Anderson, G.W.; Garrard, E.H.

1963-06-01

A study was undertaken to establish the survival pattern of 18 Salmonella species in frozen whole egg exposed to gamma radiation from a Co/sup 60/ source as part of a program to investigate the possibilities of using radiation for the destruction of micro-organisms in foods. A definite variation in sensitivity to radiation was found among these 18 species commonly occurring in frozen egg. The dose levels, applied at a dose rate of 0.491 Mrad/hr, required for a 10/sup 7/-fold reduction of these species ranged from 0.36 to 0.54 Mrad. Variation in sensitivity to radiation was also detected with three culturesmore » of S. pullorum that appeared similar except in origin. Variation in sensitivity within all species of Salmonella studied apparently indicates that no one species can be considered most resistant. These studies reveal that a level of 0.54 Mrad of gamma radiation would reduce the salmonellae to a safe level in frozen egg. However, this is based on the supposition that 10/sup 5/ cells is a reasonable number to consider necessary for the initiation of salmonellosis. The most heavily contaminated frozen egg melange examined contained approximates 2 x 10/sup 5/ cells of salmonellae/ml. Of these, any process giving a 10/sup 7/-fold reduction would leave only 2 x 10/sup -2/ cells/ml, i.e., 2 cells/100 ml or about 10 cells per lb. Apparently it would be necessary to consume several tons of uncooked melange to obtain an infective dose and therefore the 10/sup 7/-fold reduction is adequate from the viewpoint of the public health hazard. Although off-flavors develop in frozen whole egg irradiated with 0.5 Mrad, they disappear on storage and the irradiated product was found acceptable for commercial use. Thus irradiation of frozen whole egg melange with 0.54 Mrad would not be expected to markedly increase the unfavorable alterations in the product, indicating that gamma radiation possesses a distinct potential for removing Salmonella from frozen whole egg melange. (BBB)« less

PNNL Researchers Collect Permafrost Cores in Alaska

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2016-11-23

Permafrost is ground that is frozen for two or more years. In the Arctic, discontinuous regions of this saturated admixture of soil and rock store a large fraction of the Earth’s carbon – about 1672 petagrams (1672 trillion kilograms). As temperatures increase in the Northern Hemisphere, a lot of that carbon may be released to the atmosphere, making permafrost an important factor to represent accurately in global climate models. At Pacific Northwest National Laboratory, a group led by James C. Stegen periodically extracts permafrost core samples from a site near Fairbanks, Alaska. Back at the lab in southeastern Washington State,more » they study the cores for levels of microbial activity, carbon fluxes, hydrologic patterns, and other factors that reveal the dynamics of this consequential layer of soil and rock.« less

Thinking Like a Wildcatter: Prospecting for Methane in Arabia Terra, Mars

NASA Technical Reports Server (NTRS)

Allen, C. C.; Oehler, D. Z.

2005-01-01

Methane has been detected in the martian atmosphere at a concentration of approximately 10 ppb. The lifetime of such methane against decomposition by solar radiation is approximately 300 years, strongly suggesting that methane is currently being released to the atmosphere. By analogy to Earth, possible methane sources on Mars include active volcanism, hot springs, frozen methane clathrates, thermally-matured sedimentary organic matter, and extant microbial metabolism. The discovery of any one of these sources would revolutionize our understanding of Mars.

Patient and cycle characteristics predicting high pregnancy rates with single-embryo transfer: an analysis of the Society for Assisted Reproductive Technology outcomes between 2004 and 2013.

PubMed

Mersereau, Jennifer; Stanhiser, Jamie; Coddington, Charles; Jones, Tiffany; Luke, Barbara; Brown, Morton B

2017-11-01

To analyze factors associated with high live birth rate and low multiple birth rate in fresh and frozen-thawed assisted reproductive technology (ART) cycles. Retrospective cohort analysis. Not applicable. The study population included 181,523 women undergoing in vitro fertilization with autologous fresh first cycles, 27,033 with fresh first oocyte donor cycles, 37,658 with fresh second cycles, and 35,446 with frozen-thawed second cycles. None. Live birth rate and multiple birth rate after single-embryo transfer (SET) and double embryo transfer (DET) were measured, in addition to cycle characteristics. In patients with favorable prognostic factors, including younger maternal age, transfer of a blastocyst, and additional embryos cryopreserved, the gain in the live birth rate from SET to DET was approximately 10%-15%; however, the multiple birth rate increased from approximately 2% to greater than 49% in both autologous and donor fresh and frozen-thawed transfer cycles. This study reports a 10%-15% reduction in live birth rate and a 47% decrement in multiple birth rate with SET compared with DET in the setting of favorable patient prognostic factors. Our findings present an opportunity to increase the rate of SET across the United States and thereby reduce the multiple birth rate and its associated poor perinatal outcomes with assisted reproductive technology pregnancies. Copyright © 2017 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

The Physics of Ice Sheets

ERIC Educational Resources Information Center

Bassis, J. N.

2008-01-01

The great ice sheets in Antarctica and Greenland are vast deposits of frozen freshwater that contain enough to raise sea level by approximately 70 m if they were to completely melt. Because of the potentially catastrophic impact that ice sheets can have, it is important that we understand how ice sheets have responded to past climate changes and…

Evaluation of Catfish Skin Hydrolysates as a Glazing Material for Air-Blast Frozen Shrimp

USDA-ARS?s Scientific Manuscript database

Catfish is one of the most widely consumed seafood in the United States. A by-product of this consumption is a large quantity of catfish skin (CS), approximately 8,200 metric tons in 2014. Enzymatic hydrolysis is used to produce protein hydrolysates from the skin. These hydrolysates have considerabl...

Cold-stage microscopy system for fast-frozen liquids.

PubMed

Talmon, Y; Davis, H T; Scriven, L E; Thomas, E L

1979-06-01

The least artifact-laden fixation technique for examining colloidal suspensions, microemulsions, and other microstructured liquids in the electron microscope appears to be thermal fixation, i.e., ultrafast freezing of the liquid specimen. For rapid-enough cooling and for observation in TEM/STEM a thin sample is needed. The need is met by trapping a thin layer ( approximately 100 nm) of liquid between two polyimide films ( approximately 40 nm thickness) mounted on copper grids and immersing the resulting sandwich in liquid nitrogen at its melting point. For liquids containing water, polyimides films are used since this polymer is far less susceptible to the electron beam damage observed for the commonly used polymer films such as Formvar and collodion in contact with ice. Transfer of the frozen sample into the microscope column without deleterious frost deposition and warming is accomplished with a new transfer module for the cooling stage of the JEOL JEM-100CX microscope, which makes a true cold stage out of a device originally intended for cooling specimens inside the column. Sample results obtained with the new fast-freeze, cold-stage microscopy system are given.

Analytic energy gradients for the coupled-cluster singles and doubles method with the density-fitting approximation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bozkaya, Uğur, E-mail: ugur.bozkaya@hacettepe.edu.tr; Department of Chemistry, Atatürk University, Erzurum 25240; Sherrill, C. David

2016-05-07

An efficient implementation is presented for analytic gradients of the coupled-cluster singles and doubles (CCSD) method with the density-fitting approximation, denoted DF-CCSD. Frozen core terms are also included. When applied to a set of alkanes, the DF-CCSD analytic gradients are significantly accelerated compared to conventional CCSD for larger molecules. The efficiency of our DF-CCSD algorithm arises from the acceleration of several different terms, which are designated as the “gradient terms”: computation of particle density matrices (PDMs), generalized Fock-matrix (GFM), solution of the Z-vector equation, formation of the relaxed PDMs and GFM, back-transformation of PDMs and GFM to the atomic orbitalmore » (AO) basis, and evaluation of gradients in the AO basis. For the largest member of the alkane set (C{sub 10}H{sub 22}), the computational times for the gradient terms (with the cc-pVTZ basis set) are 2582.6 (CCSD) and 310.7 (DF-CCSD) min, respectively, a speed up of more than 8-folds. For gradient related terms, the DF approach avoids the usage of four-index electron repulsion integrals. Based on our previous study [U. Bozkaya, J. Chem. Phys. 141, 124108 (2014)], our formalism completely avoids construction or storage of the 4-index two-particle density matrix (TPDM), using instead 2- and 3-index TPDMs. The DF approach introduces negligible errors for equilibrium bond lengths and harmonic vibrational frequencies.« less

Potential microbial contamination during sampling of permafrost soil assessed by tracers

NASA Astrophysics Data System (ADS)

Bang-Andreasen, Toke; Schostag, Morten; Priemé, Anders; Elberling, Bo; Jacobsen, Carsten S.

2017-02-01

Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. A protocol with special emphasis on avoiding microbial contamination was developed and employed to examine how far microbial contamination can penetrate into permafrost cores. The quantity of tracer elements decreased with depth into the permafrost cores, but the tracers were detected as far as 17 mm from the surface of the cores. The results emphasize that caution should be taken to avoid microbial contamination of permafrost cores and that the application of tracers represents a useful tool to assess penetration of potential microbial contamination into permafrost cores.

Potential microbial contamination during sampling of permafrost soil assessed by tracers.

PubMed

Bang-Andreasen, Toke; Schostag, Morten; Priemé, Anders; Elberling, Bo; Jacobsen, Carsten S

2017-02-23

Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. A protocol with special emphasis on avoiding microbial contamination was developed and employed to examine how far microbial contamination can penetrate into permafrost cores. The quantity of tracer elements decreased with depth into the permafrost cores, but the tracers were detected as far as 17 mm from the surface of the cores. The results emphasize that caution should be taken to avoid microbial contamination of permafrost cores and that the application of tracers represents a useful tool to assess penetration of potential microbial contamination into permafrost cores.

Potential microbial contamination during sampling of permafrost soil assessed by tracers

PubMed Central

Bang-Andreasen, Toke; Schostag, Morten; Priemé, Anders; Elberling, Bo; Jacobsen, Carsten S.

2017-01-01

Drilling and handling of permanently frozen soil cores without microbial contamination is of concern because contamination e.g. from the active layer above may lead to incorrect interpretation of results in experiments investigating potential and actual microbial activity in these low microbial biomass environments. Here, we present an example of how microbial contamination from active layer soil affected analysis of the potentially active microbial community in permafrost soil. We also present the development and use of two tracers: (1) fluorescent plastic microspheres and (2) Pseudomonas putida genetically tagged with Green Fluorescent Protein production to mimic potential microbial contamination of two permafrost cores. A protocol with special emphasis on avoiding microbial contamination was developed and employed to examine how far microbial contamination can penetrate into permafrost cores. The quantity of tracer elements decreased with depth into the permafrost cores, but the tracers were detected as far as 17 mm from the surface of the cores. The results emphasize that caution should be taken to avoid microbial contamination of permafrost cores and that the application of tracers represents a useful tool to assess penetration of potential microbial contamination into permafrost cores. PMID:28230151

Latent injury in frozen-thawed bacteriophage T4Bo.

PubMed Central

Steele, P. R.

1976-01-01

Two interesting new phenomena have been observed in suspensions of T4Bo bacteriophage which were frozen to temperatures below the eutectic temperature of the salt (sodium chloride) in the suspending medium. Approximately 10% of the phage appeared to survive such a phase change as determined by plaque titre. However, exposure of these survivors to ultrasonic vibration or repeated freezing showed them to be hypersensitive and thus latently injured. The hypersensitivity was lost on incubating the phage at 37 degrees C. for 3 hr. Furthermore, following a eutectic phase change, the surviving phage could be inactivated by rapid cooling to -90 degrees C. followed by slow rewarming. Such inactivation cannot be accounted for by accepted theories of freezing injury. PMID:1068188

Light induces a rapid and transient increase in inositol-trisphosphate in toad rod outer segments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, J.E.; Blazynski, C.; Cohen, A.I.

1987-08-14

The sub-second time course of changes in the content of (/sup 3/H)inositol-1,4,5-trisphosphate was determined in rod outer segments from very rapidly frozen Bufo retinas that had been incubated with (/sup 3/H)inositol. Rod outer segments were cut off frozen specimens with a cryostat microtome and the water soluble extracts were analyzed. The content of (/sup 3/H)inositol-1,4,5-trisphosphate rose after approximately 250 msec of bright illumination, but returned to the unstimulated level after 1 sec, whether the stimulus remained on or not. That is, there was rapid but transient change in the content of (/sup 3/H)inositol-1,4,5-trisphosphate after the onset of stimulation.

On the coupling of fluid dynamics and electromagnetism at the top of the earth's core

NASA Technical Reports Server (NTRS)

Benton, E. R.

1985-01-01

A kinematic approach to short-term geomagnetism has recently been based upon pre-Maxwell frozen-flux electromagnetism. A complete dynamic theory requires coupling fluid dynamics to electromagnetism. A geophysically plausible simplifying assumption for the vertical vorticity balance, namely that the vertical Lorentz torque is negligible, is introduced and its consequences are developed. The simplified coupled magnetohydrodynamic system is shown to conserve a variety of magnetic and vorticity flux integrals. These provide constraints on eligible models for the geomagnetic main field, its secular variation, and the horizontal fluid motions at the top of the core, and so permit a number of tests of the underlying assumptions.

Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica.

PubMed

Dieni, Christopher A; Storey, Kenneth B

2008-04-22

The wood frog, Rana sylvatica, is one of a few vertebrate species that have developed natural freeze tolerance, surviving days or weeks with 65-70% of its total body water frozen in extracellular ice masses. Frozen frogs exhibit no vital signs and their organs must endure multiple stresses, particularly long term anoxia and ischemia. Maintenance of cellular energy supply is critical to viability in the frozen state and in skeletal muscle, AMP deaminase (AMPD) plays a key role in stabilizing cellular energetics. The present study investigated AMPD control in wood frog muscle. Wood frog AMPD was subject to multiple regulatory controls: binding to subcellular structures, protein phosphorylation, and effects of allosteric effectors, cryoprotectants and temperature. The percentage of bound AMPD activity increased from 20 to 35% with the transition to the frozen state. Bound AMPD showed altered kinetic parameters compared with the free enzyme (S0.5 AMP was reduced, Hill coefficient fell to approximately 1.0) and the transition to the frozen state led to a 3-fold increase in S0.5 AMP of the bound enzyme. AMPD was a target of protein phosphorylation. Bound AMPD from control frogs proved to be a low phosphate form with a low S0.5 AMP and was phosphorylated in incubations that stimulated PKA, PKC, CaMK, or AMPK. Bound AMPD from frozen frogs was a high phosphate form with a high S0.5 AMP that was reduced under incubation conditions that stimulated protein phosphatases. Frog muscle AMPD was activated by Mg.ATP and Mg.ADP and inhibited by Mg.GTP, KCl, NaCl and NH4Cl. The enzyme product, IMP, uniquely inhibited only the bound (phosphorylated) enzyme from muscle of frozen frogs. Activators and inhibitors differentially affected the free versus bound enzyme. S0.5 AMP of bound AMPD was also differentially affected by high versus low assay temperature (25 vs 5 degrees C) and by the presence/absence of the natural cryoprotectant (250 mM glucose) that accumulates during freezing. Maintenance of long term viability under the ischemic conditions in frozen muscle requires attention to the control of cellular energetics. Differential regulatory controls on AMPD by mechanisms including binding to muscle proteins, actions allosteric effectors, glucose and temperature effects and reversible phosphorylation adjust enzyme function for an optimal role in controlling cellular adenylate levels in ischemic frozen muscle. Stable modification of AMPD properties via freeze-responsive phosphorylation may contribute both to AMPD control and to coordinating AMPD function with other enzymes of energy metabolism in cold ischemic muscle.

Nonuniform continuum model for solvatochromism based on frozen-density embedding theory.

PubMed

Shedge, Sapana Vitthal; Wesolowski, Tomasz A

2014-10-20

Frozen-density embedding theory (FDET) provides the formal framework for multilevel numerical simulations, such that a selected subsystem is described at the quantum mechanical level, whereas its environment is described by means of the electron density (frozen density; ${\\rho _{\\rm{B}} (\\vec r)}$). The frozen density ${\\rho _{\\rm{B}} (\\vec r)}$ is usually obtained from some lower-level quantum mechanical methods applied to the environment, but FDET is not limited to such choices for ${\\rho _{\\rm{B}} (\\vec r)}$. The present work concerns the application of FDET, in which ${\\rho _{\\rm{B}} (\\vec r)}$ is the statistically averaged electron density of the solvent ${\\left\\langle {\\rho _{\\rm{B}} (\\vec r)} \\right\\rangle }$. The specific solute-solvent interactions are represented in a statistical manner in ${\\left\\langle {\\rho _{\\rm{B}} (\\vec r)} \\right\\rangle }$. A full self-consistent treatment of solvated chromophore, thus involves a single geometry of the chromophore in a given state and the corresponding ${\\left\\langle {\\rho _{\\rm{B}} (\\vec r)} \\right\\rangle }$. We show that the coupling between the two descriptors might be made in an approximate manner that is applicable for both absorption and emission. The proposed protocol leads to accurate (error in the range of 0.05 eV) descriptions of the solvatochromic shifts in both absorption and emission. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Preliminary results of the first scientific Drilling on Lake Baikal, Buguldeika site, southeastern Siberia

USGS Publications Warehouse

Williams, Douglas F.; Colman, S.; Grachev, M.; Hearn, P.; Horie, Shoji; Kawai, T.; Kuzmin, Mikhail I.; Logachov, N.; Antipin, V.; Bardardinov, A.; Bucharov, A.; Fialkov, V.; Gorigljad, A.; Tomilov, B.; Khakhaev, B.N.; Kochikov, S.; Logachev, N.; Pevzner, L.A.; Karabanov, E.B.; Mats, V.; Baranova, E.; Khlystov, O.; Khrachenko, E.; Shimaraeva, M.; Stolbova, E.; Efremova, S.; Gvozdkov, A.; Kravchinski, A.; Peck, J.; Fileva, T.; Kashik, S.; Khramtsova, T.; Kalashnikova, I.; Rasskazova, T.; Tatarnikova, V.; Yuretich, Richard; Mazilov, V.; Takemura, K.; Bobrov, V.; Gunicheva, T.; Haraguchi, H.; Ito, S.; Kocho, T.; Markova, M.; Pampura, V.; Proidakova, O.; Ishiwatari, R.; Sawatari, H.; Takeuchi, A.; Toyoda, K.; Vorobieva, S.; Ikeda, A.; Marui, A.; Nakamura, T.; Ogura, K.; Ohta, Takeshi; King, J.; Sakai, H.; Yokoyama, T.; Hayashida, A.; Bezrukova, E.; Fowell, S.; Fujii, N.; Letunova, P.; Misharina, V.; Miyoshi, N.; Chernyaeva, G.; Ignatova, I.; Likhoshvai, E.; Granina, L.; Levina, O.; Dolgikh, P.; Lazo, F.; Lutskaia, N.; Orem, W.; Wada, E.; Yamada, K.; Yamada, S.; Callander, E.; Golobokoval, L.; Shanks, W. C. Pat; Dorofeeva, R.; Duchkov, A.

1997-01-01

The Baikal Drilling Project (BDP) is a multinational effort to investigate the paleoclimatic history and tectonic evolution of the Baikal sedimentary basin during the Late Neogene. In March 1993 the Baikal drilling system was successfuly deployed from a barge frozen into position over a topographic high, termed the Buguldeika saddle, in the southern basin of Lake Baikal. The BDP-93 scientific team, made up of Russian, American and Japanese scientists, successfully recovered the first long (>100 m) hydraulic piston cores from two holes in 354 m of water. High quality cores of 98 m (Hole 1) and 102 m (Hole 2), representing sedimentation over the last 500,000 years, were collected in 78 mm diameter plastic liners with an average recovery of 72% and 90%, respectively. Magnetic susceptibility logging reveals an excellent hole-to-hole correlation. In this report the scientific team describes the preliminary analytical results from BDP-93 hole 1 cores. Radiocarbon dating by accelerator mass spectrometry provides an accurate chronology for the upper portion of Hole 1. Detailed lithologic characteristics, rock magnetic properties and inorganic element distributions show a significant change to the depositional environment occuring at 50 m subbottom depth, approximately 250,000 BP. This change may be due to uplift and rotation of the horst block in the Buguldeika saddle. The sedimentary section above 50 m is pelitic with varve-like laminae, whereas the section below 50 m contains a high proportion of sand and gravel horizons often organized into turbidite sequences. Accordingly, high resolution seismic records reveal a change in sonic velocity at this depth. It is inferred that sedimentation prior to 250 ka BP was from the west via the Buguldeika river system. After 250 ka BP the Buguldeika saddle reflects an increase in hemipelagic sediments admixed with fine-grained material from the Selenga River drainage basin, east of Lake Baikal. Variations in the spore-pollen assemblage, diatoms, biogenic silica content, rock magnetic properties, clay mineralogy and organic carbon in the upper 50 m of BDP-93-1 reveal a detailed record of climate change over approximately the last 250,000 years. These variables alternate in a pattern characteristic of glacial/interglacial climatic fluctuations. The present age model suggests that the climate signal recorded in Lake Baikal sediments is similar to Late Quaternary signals recorded in Chinese loess sections and in marine sediments.

The PSEUDODOJO: Training and grading a 85 element optimized norm-conserving pseudopotential table

NASA Astrophysics Data System (ADS)

van Setten, M. J.; Giantomassi, M.; Bousquet, E.; Verstraete, M. J.; Hamann, D. R.; Gonze, X.; Rignanese, G.-M.

2018-05-01

First-principles calculations in crystalline structures are often performed with a planewave basis set. To make the number of basis functions tractable two approximations are usually introduced: core electrons are frozen and the diverging Coulomb potential near the nucleus is replaced by a smoother expression. The norm-conserving pseudopotential was the first successful method to apply these approximations in a fully ab initio way. Later on, more efficient and more exact approaches were developed based on the ultrasoft and the projector augmented wave formalisms. These formalisms are however more complex and developing new features in these frameworks is usually more difficult than in the norm-conserving framework. Most of the existing tables of norm-conserving pseudopotentials, generated long ago, do not include the latest developments, are not systematically tested or are not designed primarily for high precision. In this paper, we present our PSEUDODOJO framework for developing and testing full tables of pseudopotentials, and demonstrate it with a new table generated with the ONCVPSP approach. The PSEUDODOJO is an open source project, building on the ABIPY package, for developing and systematically testing pseudopotentials. At present it contains 7 different batteries of tests executed with ABINIT, which are performed as a function of the energy cutoff. The results of these tests are then used to provide hints for the energy cutoff for actual production calculations. Our final set contains 141 pseudopotentials split into a standard and a stringent accuracy table. In total around 70,000 calculations were performed to test the pseudopotentials. The process of developing the final table led to new insights into the effects of both the core-valence partitioning and the non-linear core corrections on the stability, convergence, and transferability of norm-conserving pseudopotentials. The PSEUDODOJO hence provides a set of pseudopotentials and general purpose tools for further testing and development, focusing on highly accurate calculations and their use in the development of ab initio packages. The pseudopotential files are available on the PSEUDODOJO web-interface pseudo-dojo.org under the name NC (ONCVPSP) v0.4 in the psp8, UPF2, and PSML 1.1 formats. The webinterface also provides the inputs, which are compatible with the 3.3.1 and higher versions of ONCVPSP. All tests have been performed with ABINIT 8.4.

Freeze core sampling to validate time-lapse resistivity monitoring of the hyporheic zone.

PubMed

Toran, Laura; Hughes, Brian; Nyquist, Jonathan; Ryan, Robert

2013-01-01

A freeze core sampler was used to characterize hyporheic zone storage during a stream tracer test. The pore water from the frozen core showed tracer lingered in the hyporheic zone after the tracer had returned to background concentration in collocated well samples. These results confirmed evidence of lingering subsurface tracer seen in time-lapse electrical resistivity tomographs. The pore water exhibited brine exclusion (ion concentrations in ice lower than source water) in a sediment matrix, despite the fast freezing time. Although freeze core sampling provided qualitative evidence of lingering tracer, it proved difficult to quantify tracer concentration because the amount of brine exclusion during freezing could not be accurately determined. Nonetheless, the additional evidence for lingering tracer supports using time-lapse resistivity to detect regions of low fluid mobility within the hyporheic zone that can act as chemically reactive zones of importance in stream health. © 2012, The Author(s). GroundWater © 2012, National Ground Water Association.

The magnetic field at the core-mantle boundary

NASA Technical Reports Server (NTRS)

Bloxham, J.; Gubbins, D.

1985-01-01

Models of the geomagnetic field are, in general, produced from a least-squares fit of the coefficients in a truncated spherical harmonic expansion to the available data. Downward continuation of such models to the core-mantle boundary (CMB) is an unstable process: the results are found to be critically dependent on the choice of truncation level. Modern techniques allow this fundamental difficulty to be circumvented. The method of stochastic inversion is applied to modeling the geomagnetic field. Prior information is introduced by requiring that the spectrum of spherical harmonic coefficients to fall-off in a particular manner which is consistent with the Ohmic heating in the core having a finite lower bound. This results in models with finite errors in the radial field at the CMB. Curves of zero radial field can then be determined and integrals of the radial field over patches on the CMB bounded by these null-flux curves calculated. With the assumption of negligible magnetic diffusion in the core; frozen-flux hypothesis, these integrals are time-invariant.

The effects of freezing and thawing rates on tenderness, sensory quality, and retail display of beef subprimals.

PubMed

Hergenreder, J E; Hosch, J J; Varnold, K A; Haack, A L; Senaratne, L S; Pokharel, S; Beauchamp, C; Lobaugh, B; Calkins, C R

2013-01-01

The objective of this study was to evaluate processing methods for frozen beef subprimals; the effects of freezing and thawing rates on tenderness, sensory properties, and retail display were evaluated. There were 6 treatments: fresh, never frozen 14 d wet aged (14D); fresh, never frozen 21 d wet aged (21D); blast frozen-fast thawed (BF); blast frozen-slow thawed (BS); conventionally frozen-fast thawed (CF); and conventionally frozen-slow thawed (CS). All frozen beef subprimals were aged for 14 d before freezing. Three beef subprimal cuts, rib eye roll (n=90), strip loin (n=90), and top sirloin butt (n=90), were used with 3 replications of 5 samples per treatment per week (total of 9 wk, n=270). Blast freezing occurred by placing spacers between the boxes of meat on pallets at -28°C with high air velocity for 3 to 5 d. Conventional freezing occurred with boxes of meat stacked on pallets and placed in a -28°C freezer with minimal air movement for at least 10 d. Fast thawing of subprimals (to an internal temperature of -1°C to 1°C) occurred by immersion in a circulating water bath (<12°C) for 21 h, and slow thawing of subprimals occurred over a 2-wk period by placing individual subprimals on tables at 0°C. Steaks (2.5 cm thick) were cut from the longissimus thoracis (LT), longissimus lumborum (LL), and gluteus medius (GM) for Warner-Bratzler shear force (WBS), trained sensory evaluation, and retail display. For LL and GM beef steaks, frozen treatments were equal or lower in WBS values to 14D and 21D beef steaks. No differences were detected in WBS among the treatments applied to GM beef steaks (P=0.08). There were no differences in sensory tenderness among the LL, LT, and GM (P>0.05). All LL and LT beef steaks had approximately 4 d to 40% discoloration, and all GM steaks had over 3 d to 40% discoloration. Steaks from the LL and LT began to discolor at about 3 d, and the GM began to discolor after 1 d. For all beef subprimals, purge loss during storage and thawing was significantly greater for the slow-thawed subprimals (P<0.01), and all fast-thawed subprimals were equal or superior to 14D and 21D (P<0.01) in storage and thawing purge. During retail display, the greatest purge loss occurred in fast-thawed treatments (P<0.01). Overall, freezing rate did not affect purge loss, and neither freezing nor thawing rates had significant meaningful effects on WBS, and sensory properties were comparable with fresh, never-frozen subprimals.

The importance of heat flow direction for reproducible and homogeneous freezing of bulk protein solutions.

PubMed

Rodrigues, Miguel A; Balzan, Gustavo; Rosa, Mónica; Gomes, Diana; de Azevedo, Edmundo G; Singh, Satish K; Matos, Henrique A; Geraldes, Vítor

2013-01-01

Freezing is an important operation in biotherapeutics industry. However, water crystallization in solution, containing electrolytes, sugars and proteins, is difficult to control and usually leads to substantial spatial solute heterogeneity. Herein, we address the influence of the geometry of freezing direction (axial or radial) on the heterogeneity of the frozen matrix, in terms of local concentration of solutes and thermal history. Solutions of hemoglobin were frozen radially and axially using small-scale and pilot-scale freezing systems. Concentration of hemoglobin, sucrose and pH values were measured by ice-core sampling and temperature profiles were measured at several locations. The results showed that natural convection is the major source for the cryoconcentration heterogeneity of solutes over the geometry of the container. A significant improvement in this spatial heterogeneity was observed when the freezing geometry was nonconvective, i.e., the freezing front progression was unidirectional from bottom to top. Using this geometry, less than 10% variation in solutes concentration was obtained throughout the frozen solutions. This result was reproducible, even when the volume was increased by two orders of magnitude (from 30 mL to 3 L). The temperature profiles obtained for the nonconvective freezing geometry were predicted using a relatively simple computational fluid dynamics model. The reproducible solutes distribution, predictable temperature profiles, and scalability demonstrate that the bottom to top freezing geometry enables an extended control over the freezing process. This geometry has therefore shown the potential to contribute to a better understanding and control of the risks inherent to frozen storage. © 2013 American Institute of Chemical Engineers.

Transportation of perishable and refrigerated foods in mylar foil bags and insulated containers: a time-temperature study.

PubMed

Li, Yanyan; Schrade, John P; Su, Haiyan; Specchio, John J

2014-08-01

Data are lacking on the temperature changes of food during transport without the use of refrigerated trucks. The purpose of this study was to evaluate the ability of several insulated and noninsulated containers with or without frozen gel packs to keep perishable and refrigerated foods within the temperature safe zone in relationship to duration of transport. The study was designed to duplicate the practices exhibited by customers purchasing perishable food products from a cash-and-carry business. Approximately 40 perishable food items were evaluated. Four types of containers were tested: a mylar foil bag, a commercial insulated bag, a generic insulated bag, and a commercial insulated blanket. Mixed foods were placed into these containers with or without frozen gel packs, transported in unrefrigerated vehicles, and monitored for 4 h for temperature changes. Two environmental temperatures, room temperature of 21.1°C and a stress temperature of 37.8°C, were evaluated. The internal temperature and surface temperature of the food products in these containers increased slowly but remained well below the U.S. Food and Drug Administration Food Code requirements. The various containers were similar in their ability to retain coolness. The presence of frozen gel packs dramatically enhanced the cold-holding capacity of the containers. The temperature of foods increased more rapidly when stressed in a heated environment. The containers tested used with the frozen gel packs can keep the surface and internal temperatures of various perishable foods (starting at 4.4°C or less) within the Food Code recommendation of under 21.1°C for 4 h. Cash-and-carry businesses should strongly encourage their retail customers to utilize these containers with frozen gel packs to safely transport perishable foods.

Laboratory Determination of the Infrared Band Strengths of Pyrene Frozen in Water Ice: Implications for the Composition of Interstellar Ices

NASA Technical Reports Server (NTRS)

Hardegree-Ullman, E.E.; Gudipati, M.S.; Boogert, A.C.A.; Lignell, H.; Allamandola, L.J.; Stapelfeldt, K. R.; Werner, M.

2014-01-01

Broad infrared emission features (e.g., at 3.3, 6.2, 7.7, 8.6, and 11.3 micrometers) from the gas phase interstellar medium have long been attributed to polycyclic aromatic hydrocarbons (PAHs). A significant portion (10 to 20%) of the Milky Way's carbon reservoir is locked in PAH molecules, which makes their characterization integral to our understanding of astrochemistry. In molecular clouds and the dense envelopes and disks of young stellar objects (YSOs), PAHs are expected to be frozen in the icy mantles of dust grains where they should reveal themselves through infrared absorption. To facilitate the search for frozen interstellar PAHs, laboratory experiments were conducted to determine the positions and strengths of the bands of pyrene mixed with H2O and deuterium oxide ices. The deuterium oxide mixtures are used to measure pyrene bands that are masked by the strong bands of H2O, leading to the first laboratory determination of the band strength for the CH stretching mode of pyrene in water ice near 3.25 micrometers. Our infrared band strengths were normalized to experimentally determined ultraviolet (UV) band strengths, and we find that they are generally approximately 50% larger than those reported by Bouwman et al. (2011) based on theoretical strengths. These improved band strengths were used to reexamine YSO spectra published by Boogert et al. (2008) to estimate the contribution of frozen PAHs to absorption in the 5 to 8 micrometer spectral region, taking into account the strength of the 3.25 micrometer CH stretching mode. It is found that frozen neutral PAHs contain 5 to 9% of the cosmic carbon budget, and account for 2 to 9% of the unidentified absorption in the 5 to 8 micrometer region.

HPTAM, a two-dimensional Heat Pipe Transient Analysis Model, including the startup from a frozen state

NASA Technical Reports Server (NTRS)

Tournier, Jean-Michel; El-Genk, Mohamed S.

1995-01-01

A two-dimensional Heat Pipe Transient Analysis Model, 'HPTAM,' was developed to simulate the transient operation of fully-thawed heat pipes and the startup of heat pipes from a frozen state. The model incorporates: (a) sublimation and resolidification of working fluid; (b) melting and freezing of the working fluid in the porous wick; (c) evaporation of thawed working fluid and condensation as a thin liquid film on a frozen substrate; (d) free-molecule, transition, and continuum vapor flow regimes, using the Dusty Gas Model; (e) liquid flow and heat transfer in the porous wick; and (f) thermal and hydrodynamic couplings of phases at their respective interfaces. HPTAM predicts the radius of curvature of the liquid meniscus at the liquid-vapor interface and the radial location of the working fluid level (liquid or solid) in the wick. It also includes the transverse momentum jump condition (capillary relationship of Pascal) at the liquid-vapor interface and geometrically relates the radius of curvature of the liquid meniscus to the volume fraction of vapor in the wick. The present model predicts the capillary limit and partial liquid recess (dryout) in the evaporator wick, and incorporates a liquid pooling submodel, which simulates accumulation of the excess liquid in the vapor core at the condenser end.

Nonlinear core deflection in injection molding

NASA Astrophysics Data System (ADS)

Poungthong, P.; Giacomin, A. J.; Saengow, C.; Kolitawong, C.; Liao, H.-C.; Tseng, S.-C.

2018-05-01

Injection molding of thin slender parts is often complicated by core deflection. This deflection is caused by molten plastics race tracking through the slit between the core and the rigid cavity wall. The pressure of this liquid exerts a lateral force of the slender core causing the core to bend, and this bending is governed by a nonlinear fifth order ordinary differential equation for the deflection that is not directly in the position along the core. Here we subject this differential equation to 6 sets of boundary conditions, corresponding to 6 commercial core constraints. For each such set of boundary conditions, we develop an explicit approximate analytical solution, including both a linear term and a nonlinear term. By comparison with finite difference solutions, we find our new analytical solutions to be accurate. We then use these solutions to derive explicit analytical approximations for maximum deflections and for the core position of these maximum deflections. Our experiments on the base-gated free-tip boundary condition agree closely with our new explicit approximate analytical solution.

Geology and environments of subglacial Lake Vostok.

PubMed

Leitchenkov, German L; Antonov, Anton V; Luneov, Pavel I; Lipenkov, Vladimir Ya

2016-01-28

The reconstruction of the geological (tectonic) structure and environments of subglacial Lake Vostok is based on geophysical surveys and the study of mineral particles found in cores of accreted ice and frozen lake water (sampled after the lake was unsealed). Seismic reflection and refraction investigations conducted in the southern part of Lake Vostok show very thin (200-300 m) sedimentary cover overlying a crystalline basement. Most of this thin veneer is thought to have been deposited during temperate-glacial conditions in Oligocene to Middle Miocene time (ca 34-14 Ma). The composition of the lake-bottom sediments can be deduced from mineral inclusions found in cores of accreted ice. Inclusions are represented by soft aggregates consisting mainly of clay-mica minerals and micrometre-sized quartz grains. Some of these inclusions contain subangular to semi-rounded rock clasts (siltstones and sandstones) ranging from 0.3 to 8 mm in size. In total, 31 zircon grains have been identified in two rock clasts and dated using SHRIMP-II. The ages of the studied zircons range from 0.6 to 2.0 Ga with two distinct clusters between 0.8 and 1.15 Ga and between 1.6 and 1.8 Ga. Rock clasts obviously came from the western lake shore, which is thus composed of terrigenous strata with an age of not older than 600 Ma. The sedimentary nature of the western lake shore is also confirmed by seismic refraction data showing seismic velocities there of 5.4-5.5 km s(-1) at the bedrock surface. After Lake Vostok was unsealed, its water (frozen and sampled next season) was also studied with scanning electron microscopy and X-ray microprobe analysis. This study showed the existence of calcium carbonate and silica microparticles (10-20 μm across) in frozen water. © 2015 The Author(s).

Molecular analysis of HER2 signaling in human breast cancer by functional protein pathway activation mapping

PubMed Central

Wulfkuhle, Julia D.; Berg, Daniela; Wolff, Claudia; Langer, Rupert; Tran, Kai; Illi, Julie; Espina, Virginia; Pierobon, Mariaelena; Deng, Jianghong; DeMichele, Angela; Walch, Axel; Bronger, Holger; Becker, Ingrid; Waldhör, Christine; Höfler, Heinz; Esserman, Laura; Liotta, Lance A.; Becker, Karl-Friedrich; Petricoin, Emanuel F.

2017-01-01

Purpose Targeting of the HER2 protein in human breast cancer represents a major advance in oncology, but relies on measurements of total HER2 protein and not HER2 signaling network activation. We utilized reverse phase protein microarrays (RPMAs) to measure total and phosphorylated HER2 in the context of HER family signaling to understand correlations between phosphorylated and total levels of HER2 and downstream signaling activity. Experimental Design Three independent study sets, comprising a total of 415 individual patient samples from flash frozen core biopsy samples and FFPE surgical and core samples, were analyzed via RPMA. The phosphorylation and total levels of the HER receptor family proteins and downstream signaling molecules were measured in laser capture microdissected (LCM) enriched tumor epithelium from 127 frozen pre-treatment core biopsy samples and whole tissue lysates from 288 FFPE samples and these results were compared to FISH and IHC. Results RPMA measurements of total HER2 were highly concordant (> 90% all sets) with FISH and/or IHC data, as was phosphorylation of HER2 in the FISH/IHC+ population. Phosphorylation analysis of HER family signaling identified HER2 activation in some FISH/IHC- tumors and, identical to that seen with FISH/IHC+ tumors, the HER2 activation was concordant with EGFR and HER3 phosphorylation and downstream signaling endpoint activation. Conclusions Molecular profiling of HER2 signaling of a large cohort of human breast cancer specimens using a quantitative and sensitive functional pathway activation mapping technique reveals IHC-/FISH-/pHER2+ tumors with HER2 pathway activation independent of total HER2 levels and functional signaling through HER3 and EGFR. PMID:23045247

Optimization of selected molecular orbitals in group basis sets.

PubMed

Ferenczy, György G; Adams, William H

2009-04-07

We derive a local basis equation which may be used to determine the orbitals of a group of electrons in a system when the orbitals of that group are represented by a group basis set, i.e., not the basis set one would normally use but a subset suited to a specific electronic group. The group orbitals determined by the local basis equation minimize the energy of a system when a group basis set is used and the orbitals of other groups are frozen. In contrast, under the constraint of a group basis set, the group orbitals satisfying the Huzinaga equation do not minimize the energy. In a test of the local basis equation on HCl, the group basis set included only 12 of the 21 functions in a basis set one might ordinarily use, but the calculated active orbital energies were within 0.001 hartree of the values obtained by solving the Hartree-Fock-Roothaan (HFR) equation using all 21 basis functions. The total energy found was just 0.003 hartree higher than the HFR value. The errors with the group basis set approximation to the Huzinaga equation were larger by over two orders of magnitude. Similar results were obtained for PCl(3) with the group basis approximation. Retaining more basis functions allows an even higher accuracy as shown by the perfect reproduction of the HFR energy of HCl with 16 out of 21 basis functions in the valence basis set. When the core basis set was also truncated then no additional error was introduced in the calculations performed for HCl with various basis sets. The same calculations with fixed core orbitals taken from isolated heavy atoms added a small error of about 10(-4) hartree. This offers a practical way to calculate wave functions with predetermined fixed core and reduced base valence orbitals at reduced computational costs. The local basis equation can also be used to combine the above approximations with the assignment of local basis sets to groups of localized valence molecular orbitals and to derive a priori localized orbitals. An appropriately chosen localization and basis set assignment allowed a reproduction of the energy of n-hexane with an error of 10(-5) hartree, while the energy difference between its two conformers was reproduced with a similar accuracy for several combinations of localizations and basis set assignments. These calculations include localized orbitals extending to 4-5 heavy atoms and thus they require to solve reduced dimension secular equations. The dimensions are not expected to increase with increasing system size and thus the local basis equation may find use in linear scaling electronic structure calculations.

A Core Facility for the Study of Neurotoxins of Biological Origin

DTIC Science & Technology

1987-06-15

the animals were sacrificed with an intravenous overdose of sodium pentobarbital. The spilnal cords were removed rapidly and frozen in isopentane...control muscle receives no drug during 45Ca washout. Results: Ryanodine contracture 1. The effect of replacement of sodium by choline to block Na+ for...sodium by choline lowers the concentra- tion of ryanodine to cause a contracture from 10-4 M to 10- 6M and reduces the half time to peak tension from

A frozen Gaussian approximation-based multi-level particle swarm optimization for seismic inversion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Jinglai, E-mail: jinglaili@sjtu.edu.cn; Lin, Guang, E-mail: lin491@purdue.edu; Computational Sciences and Mathematics Division, Pacific Northwest National Laboratory, Richland, WA 99352

2015-09-01

In this paper, we propose a frozen Gaussian approximation (FGA)-based multi-level particle swarm optimization (MLPSO) method for seismic inversion of high-frequency wave data. The method addresses two challenges in it: First, the optimization problem is highly non-convex, which makes hard for gradient-based methods to reach global minima. This is tackled by MLPSO which can escape from undesired local minima. Second, the character of high-frequency of seismic waves requires a large number of grid points in direct computational methods, and thus renders an extremely high computational demand on the simulation of each sample in MLPSO. We overcome this difficulty by threemore » steps: First, we use FGA to compute high-frequency wave propagation based on asymptotic analysis on phase plane; Then we design a constrained full waveform inversion problem to prevent the optimization search getting into regions of velocity where FGA is not accurate; Last, we solve the constrained optimization problem by MLPSO that employs FGA solvers with different fidelity. The performance of the proposed method is demonstrated by a two-dimensional full-waveform inversion example of the smoothed Marmousi model.« less

Update on meat irradiation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olson, D.G.

1997-12-01

The irradiation of meat and poultry in the United States is intended to eliminate pathogenic bacteria from raw product, preferably after packaging to prevent recontamination. Irradiation will also increase the shelf life of raw meat and poultry products approximately two to three times the normal shelf life. Current clearances in the United States are for poultry (fresh or frozen) at doses from 1.5 to 3.0 kGy and for fresh pork at doses from 0.3 to 1.0 kGy. A petition for the clearance of all red meat was submitted to the Food and Drug Administration (FDA) in July 1994. The petitionmore » is for clearances of fresh meat at doses from 1.5 to 4.5 kGy and for frozen meat at {approximately}2.5 to 7.5 kGy. Clearance for red meat is expected before the end of 1997. There are 28 countries that have food irradiation clearances, of which 18 countries have clearances for meat or poultry. However, there are no uniform categories or approved doses for meat and poultry among the countries that could hamper international trade of irradiated meat and poultry.« less

Deriving the nuclear shell model from first principles

NASA Astrophysics Data System (ADS)

Barrett, Bruce R.; Dikmen, Erdal; Vary, James P.; Maris, Pieter; Shirokov, Andrey M.; Lisetskiy, Alexander F.

2014-09-01

The results of an 18-nucleon No Core Shell Model calculation, performed in a large basis space using a bare, soft NN interaction, can be projected into the 0 ℏω space, i.e., the sd -shell. Because the 16 nucleons in the 16O core are frozen in the 0 ℏω space, all the correlations of the 18-nucleon system are captured by the two valence, sd -shell nucleons. By the projection, we obtain microscopically the sd -shell 2-body effective interactions, the core energy and the sd -shell s.p. energies. Thus, the input for standard shell-model calculations can be determined microscopically by this approach. If the same procedure is then applied to 19-nucleon systems, the sd -shell 3-body effective interactions can also be obtained, indicating the importance of these 3-body effective interactions relative to the 2-body effective interactions. Applications to A = 19 and heavier nuclei with different intrinsic NN interactions will be presented and discussed. The results of an 18-nucleon No Core Shell Model calculation, performed in a large basis space using a bare, soft NN interaction, can be projected into the 0 ℏω space, i.e., the sd -shell. Because the 16 nucleons in the 16O core are frozen in the 0 ℏω space, all the correlations of the 18-nucleon system are captured by the two valence, sd -shell nucleons. By the projection, we obtain microscopically the sd -shell 2-body effective interactions, the core energy and the sd -shell s.p. energies. Thus, the input for standard shell-model calculations can be determined microscopically by this approach. If the same procedure is then applied to 19-nucleon systems, the sd -shell 3-body effective interactions can also be obtained, indicating the importance of these 3-body effective interactions relative to the 2-body effective interactions. Applications to A = 19 and heavier nuclei with different intrinsic NN interactions will be presented and discussed. Supported by the US NSF under Grant No. 0854912, the US DOE under Grants Nos. DESC0008485 and DE-FG02-87ER40371, the Higher Education Council of Turkey(YOK), and the Ministry of Education and Science of Russian Fed. under contracts P521 and 14.v37.21.1297.

Thermal State Of Permafrost In Urban Environment Under Changing Climatic Conditions

NASA Astrophysics Data System (ADS)

Streletskiy, D. A.; Grebenets, V. I.; Kerimov, A. G.; Kurchatova, A.; Andruschenko, F.; Gubanov, A.

2015-12-01

Risks and damage, caused by deformation of building and constructions in cryolithozone, are growing for decades. Worsening of cryo-ecological situation and loss of engineering-geocryological safety are induced by both technogenic influences on frozen basement and climate change. In such towns on permafrost as Vorkuta, Dixon more than 60% of objects are deformed, in Yakutsk, Igarka- nearly 40%, in Norilsk, Talnakh, Mirnij 35%, in old indigenous villages - approximately 100%; more than 80% ground dams with frozen cores are in poor condition. This situation is accompanied by activation of dangerous cryogenic processes. For example in growing seasonally-thaw layer is strengthening frost heave of pipeline foundation: only on Yamburg gas condensate field (Taz Peninsula) are damaged by frost heave and cut or completely replaced 3000 - 5000 foundations of gas pipelines. Intensity of negative effects strongly depends on regional geocryology, technogenic loads and climatic trends, and in Arctic we see a temperature rise - warming, which cause permafrost temperature rise and thaw). In built areas heat loads are more diverse: cold foundations (under the buildings with ventilated cellars or near termosyphons) are close to warm areas with technogenic beddings (mainly sandy), that accumulate heat, close to underground collectors for communications, growing thaw zones around, close to storages of snows, etc. Note that towns create specific microclimate with higher air temperature. So towns are powerful technogenic (basically, thermal) presses, placed on permafrost; in cooperation with climate changes (air temperature rise, increase of precipitation) they cause permafrost degradation. The analysis of dozens of urban thermal fields, formed in variable cryological and soil conditions, showed, that nearly 70% have warming trend, 20% - cooling and in 10% of cases the situation after construction is stable. Triggered by warming of climate changes of vegetation, depth and temperature of seasonally thaw layer, summer precipitation regime and other natural parameters in combination with developing technogenesis require new strategy of the cryolithozone development.

Calculations of electric currents in Europa

NASA Technical Reports Server (NTRS)

Colburn, D. S.; Reynolds, R. T.

1986-01-01

Electrical currents should flow in the Galilean satellite, Europa, because it is located in Jupiter's corotating magnetosphere. The possible magnitudes of these currents are calculated by assuming that Europa is a differentiated body consisting of an outer H2O layer and a silicate core. Two types of models are considered here: one in which the water is completely frozen and a second in which there is an intermediate liquid layer. For the transverse electric mode (eddy currents), the calculated current density in a liquid layer is approximately 10 to the -5/Am. For the transverse magnetic mode (unipolar generator), the calculated current density in the liquid is severely constrained by the ice layer to a range of only 10 to the -10 to -11th power/ Am, for a total H2O thickness of 100 km, provided that neither layer is less than 4 km thick. The current density is less for a completely frozen H2O layer. If transient cracks were to appear in the ice layer, thereby exposing liquid, the calculated current density could rise to a range of 10 to the -6 to 10 to the -5/Am, depending on layer thicknesses, which would require an exposed area of 10 to the -9 to 10 to the -8 of the Europa surface. The corresponding total current of 2.3x10 to the 5th power A could in 1 yr. electrolyze 7x10 to the 5th power kg of water (and more if the cells were in series), and thereby store up to 10 the 8th power J of energy, but it is not clear how electrolysis can take place in the absence of suitable electrodes. Electrical heating would be significant only if the ice-layer thickness were on the order of 1 m, such as might occur if an exposed liquid surface were to freeze over; the heating under this condition could hinder the thickening of the ice layer.

Soil Water Characteristics of Cores from Low- and High-Centered Polygons, Barrow, Alaska, 2012

DOE Data Explorer

Graham, David; Moon, Ji-Won

2016-08-22

This dataset includes soil water characteristic curves for soil and permafrost in two representative frozen cores collected from a high-center polygon (HCP) and a low-center polygon (LCP) from the Barrow Environmental Observatory. Data include soil water content and soil water potential measured using the simple evaporation method for hydrological and biogeochemical simulations and experimental data analysis. Data can be used to generate a soil moisture characteristic curve, which can be fit to a variety of hydrological functions to infer critical parameters for soil physics. Considering the measured the soil water properties, the van Genuchten model predicted well the HCP, in contrast, the Kosugi model well fitted LCP which had more saturated condition.

Stability of Ribavirin for Inhalation Packaged in Syringes or Glass Vials when Stored Frozen, Refrigerated, and at Room Temperature.

PubMed

Larson, Bayli; Bushman, Lane R; Casciano, Matthew L; Oldland, Alan R; Kiser, Jennifer J; Kiser, Tyree H

2016-01-01

The primary aim of this study was to investigate ribavirin solution for inhalation stability under three different conditions (frozen, refrigerated, room temperature) over a 45-day period. A ribavirin 6000-mg vial was reconstituted with 90 mL of Sterile Water for Injection per the package insert to yield a concentration of approximately 67 mg/mL. The solution was then placed in either syringes or empty glass vials and stored in the freezer (-20°C), in the refrigerator (~0°C to 4°C), or at room temperature (~20°C to 25°C). Original concentrations were measured on day 0 and subsequent concentrations were measured on day 2, 14, and 45 utilizing a validated liquid chromatography with tandem mass spectrometry assay. All analyses were performed in triplicate for each storage condition. Additionally, at each time point the physical stability was evaluated and the pH of solution was measured. The solution was considered stable if =90% of the original concentration was retained over the study period. A validated liquid chromatography with tandem mass spectrometry analysis demonstrated that >95% of the original ribavirin concentration was preserved over the 45-day period for all study conditions. The ribavirin concentration remained within the United States Pharmacopeia (USP)-required range of 95% to 105% of the original labeled product amount throughout the entire study period for all study conditions. Precipitation of ribavirin was noted during the thawing cycle for frozen samples, but the drug went back into solution once the thawing process was completed. No changes in color or turbidity were observed in any of the prepared solutions. Values for pH remained stable over the study period and ranged from 4.1 to 5.3. Ribavirin for inhalation solution is physically and chemically stable for at least 45 days when frozen, refrigerated, or kept at room temperature after reconstitution to a concentration of approximately 67 mg/mL and placed in syringes or glass vials. Copyright© by International Journal of Pharmaceutical Compounding, Inc.

Origin, Bulk Chemical Composition and Physical Structure of the Galilean Satellites of Jupiter: A Post-Galileo Analysis

NASA Technical Reports Server (NTRS)

Prentice, A. J. R.

1999-01-01

The origin of Jupiter and the Galilean satellite system is examined in the light of the new data that has been obtained by the NASA Galileo Project. In particular, special attention is given to a theory of satellite origin which was put forward at the start of the Galileo Mission and on the basis of which several predictions have now been proven successful. These predictions concern the chemical composition of Jupiter's atmosphere and the physical structure of the satellites. According to the proposed theory of satellite origin, each of the Galilean satellites formed by chemical condensation and gravitational accumulation of solid grains within a concentric family of orbiting gas rings. These rings were cast off equatorially by the rotating proto-Jovian cloud (PJC which contracted gravitationally to form Jupiter some 4 1/2 billion years ago. The PJC formed from the gas and grains left over from the gas ring that had been shed at Jupiter's orbit by the contracting proto-solar cloud (PSC Supersonic turbulent convection provides the means for shedding discrete gas rings. The temperatures T (sub n) of the system of gas rings shed by the PSC and PJC vary with their respective mean orbital radii R (sub n) (n = 0, 1,2,...) according as T (sub n) proportional to R (sub n) (exp -0.9). If the planet Mercury condenses at 1640 K, so accounting for the high density of that planet via a process of chemical fractionation between iron and silicates, then T (sub n) at Jupiter's orbit is 158 K. Only 35% of the water vapour condenses out. Thus fractionation between rock and ice, together with an enhancement in the abundance of solids relative to gas which takes place through gravitational sedimentation of solids onto the mean orbit of the gas ring, ensures nearly equal proportions of rock and ice in each of Ganymede and Callisto. Io and Europa condense above the H20 ice point and consist solely of hydrated rock (h-rock). The Ganymedan condensate consists of h-rock and H20 ice. For Callisto, NH3 ice makes up -5% of the condensate mass next to h-rock (approximately 50%) and H2O ice (approximately 45%). Detailed thermal and structural models for each of Europa, Ganymede and Callisto are constructed on the basis of the above initial bulk chemical compositions. For Europa (E), a predicted 2-zone model consisting of a dehydrated rock core of mass 0.912 M (sub E) and a 150 km thick frozen mantle of salty H2O yields a moment-of-inertia coefficient which matches the Galileo Orbiter gravity measurement. For Ganymede (G), a 3-zone model possessing an inner core of solid FeS and mass approximately 0.116 M (sub G), and an outer H2O ice mantle of mass approximately 0.502 M (sub G) is needed to explain the gravity data. Ganymede's native magnetic field was formed by thermoremanent magnetization of Fe3O4. A new Callisto (C) model is proposed consisting of a core of mass 0.826 M (sub C) containing a uniform mixture of h-rock (60% by mass) and H2O and NH3 ices, and capped by a mantle of pure ice. This model may have the capacity to yield a thin layer of liquid NH3 (raised dot) 2H2O at the core boundary, in line with Galileo's discovery of an induced magnetic field.

Contamination Control of Freeze Shoe Coring System for Collection of Aquifer Sands

NASA Astrophysics Data System (ADS)

Homola, K.; van Geen, A.; Spivack, A. J.; Grzybowski, B.; Schlottenmier, D.

2017-12-01

We have developed and tested an original device, the freeze-shoe coring system, designed to recover undisturbed samples of water contained in sand-dominated aquifers. Aquifer sands are notoriously difficult to collect together with porewater from coincident depths, as high hydraulic permeability leads to water drainage and mixing during retrieval. Two existing corer designs were reconfigured to incorporate the freeze-shoe system; a Hydraulic Piston (HPC) and a Rotary (RC) Corer. Once deployed, liquid CO­2 contained in an interior tank is channeled to coils at the core head where it changes phase, rapidly cooling the deepest portion of the core. The resulting frozen core material impedes water loss during recovery. We conducted contamination tests to examine the integrity of cores retrieved during a March 2017 yard test deployment. Perfluorocarbon tracer (PFC) was added to the drill fluid and recovered cores were subsampled to capture the distribution of PFC throughout the core length and interior. Samples were collected from two HPC and one RC core and analyzed for PFC concentrations. The lowest porewater contamination, around 0.01% invasive fluid, occurs in the center of both HPC cores. The greatest contamination (up to 10%) occurs at the disturbed edges where core material contacts drill fluid. There was lower contamination in the core interior than top, bottom, and edges, as well as significantly lower contamination in HPC cores that those recovered with the RC. These results confirm that the freeze-shoe system, proposed for field test deployments in West Bengal, India, can successfully collect intact porewater and sediment material with minimal if any contamination from drill fluid.

Validation of a robust proteomic analysis carried out on formalin-fixed paraffin-embedded tissues of the pancreas obtained from mouse and human.

PubMed

Kojima, Kyoko; Bowersock, Gregory J; Kojima, Chinatsu; Klug, Christopher A; Grizzle, William E; Mobley, James A

2012-11-01

A number of reports have recently emerged with focus on extraction of proteins from formalin-fixed paraffin-embedded (FFPE) tissues for MS analysis; however, reproducibility and robustness as compared to flash frozen controls is generally overlooked. The goal of this study was to identify and validate a practical and highly robust approach for the proteomics analysis of FFPE tissues. FFPE and matched frozen pancreatic tissues obtained from mice (n = 8) were analyzed using 1D-nanoLC-MS(MS)(2) following work up with commercially available kits. The chosen approach for FFPE tissues was found to be highly comparable to that of frozen. In addition, the total number of unique peptides identified between the two groups was highly similar, with 958 identified for FFPE and 1070 identified for frozen, with protein identifications that corresponded by approximately 80%. This approach was then applied to archived human FFPE pancreatic cancer specimens (n = 11) as compared to uninvolved tissues (n = 8), where 47 potential pancreatic ductal adenocarcinoma markers were identified as significantly increased, of which 28 were previously reported. Further, these proteins share strongly overlapping pathway associations to pancreatic cancer that include estrogen receptor α. Together, these data support the validation of an approach for the proteomic analysis of FFPE tissues that is straightforward and highly robust, which can also be effectively applied toward translational studies of disease. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A digital rat atlas of sectional anatomy

NASA Astrophysics Data System (ADS)

Yu, Li; Liu, Qian; Bai, Xueling; Liao, Yinping; Luo, Qingming; Gong, Hui

2006-09-01

This paper describes a digital rat alias of sectional anatomy made by milling. Two healthy Sprague-Dawley (SD) rat weighing 160-180 g were used for the generation of this atlas. The rats were depilated completely, then euthanized by Co II. One was via vascular perfusion, the other was directly frozen at -85 °C over 24 hour. After that, the frozen specimens were transferred into iron molds for embedding. A 3% gelatin solution colored blue was used to fill the molds and then frozen at -85 °C for one or two days. The frozen specimen-blocks were subsequently sectioned on the cryosection-milling machine in a plane oriented approximately transverse to the long axis of the body. The surface of specimen-blocks were imaged by a scanner and digitalized into 4,600 x2,580 x 24 bit array through a computer. Finally 9,475 sectional images (arterial vessel were not perfused) and 1,646 sectional images (arterial vessel were perfused) were captured, which made the volume of the digital atlas up to 369.35 Gbyte. This digital rat atlas is aimed at the whole rat and the rat arterial vessels are also presented. We have reconstructed this atlas. The information from the two-dimensional (2-D) images of serial sections and three-dimensional (3-D) surface model all shows that the digital rat atlas we constructed is high quality. This work lays the foundation for a deeper study of digital rat.

WIND Validation Cases: Computational Study of Thermally-perfect Gases

NASA Technical Reports Server (NTRS)

DalBello, Teryn; Georgiadis, Nick (Technical Monitor)

2002-01-01

The ability of the WIND Navier-Stokes code to predict the physics of multi-species gases is investigated in support of future high-speed, high-temperature propulsion applications relevant to NASA's Space Transportation efforts. Three benchmark cases are investigated to evaluate the capability of the WIND chemistry model to accurately predict the aerodynamics of multi-species chemically non-reacting (frozen) gases. Case 1 represents turbulent mixing of sonic hydrogen and supersonic vitiated air. Case 2 consists of heated and unheated round supersonic jet exiting to ambient. Case 3 represents 2-D flow through a converging-diverging Mach 2 nozzle. For Case 1, the WIND results agree fairly well with experimental results and that significant mixing occurs downstream of the hydrogen injection point. For Case 2, the results show that the Wilke and Sutherland viscosity laws gave similar results, and the available SST turbulence model does not predict round supersonic nozzle flows accurately. For Case 3, results show that experimental, frozen, and 1-D gas results agree fairly well, and that frozen, homogeneous, multi-species gas calculations can be approximated by running in perfect gas mode while specifying the mixture gas constant and Ratio of Specific Heats.

Refractance Window™ drying of haskap berry--preliminary results on anthocyanin retention and physicochemical properties.

PubMed

Celli, Giovana Bonat; Khattab, Rabie; Ghanem, Amyl; Brooks, Marianne Su-Ling

2016-03-01

The goal of this work was to determine the anthocyanin retention and physicochemical properties of haskap powder prepared by Refractance Window™ (RW) drying. In general, the RW-dried powder particles had a smooth surface with similar thickness, consistent with the preparation method, and had a solubility of 75.63% in water. The RW-dried powder (consisting of 98% haskap berries) retained approximately 93.8% of anthocyanins from the original frozen fruits, as assessed by the pH-differential method. This result is in good agreement with HPLC analysis that indicated 92.9% retention. Three anthocyanins were identified in frozen berries and RW-dried powder: cyanidin 3-glucoside, cyanidin 3-rutinoside, and peonidin 3-glucoside. Surprisingly, cyanidin 3-rutinoside exhibited the lowest retention. Copyright © 2015 Elsevier Ltd. All rights reserved.

Permafrost Meta-Omics and Climate Change

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mackelprang, Rachel; Saleska, Scott R.; Jacobsen, Carsten Suhr

2016-06-29

Permafrost (i.e., soil that has been frozen for at least 2 consecutive years) represents a habitat for microbial life at subzero temperatures (Gilichinsky et al. 2008). Approximately one quarter of the Earth’s surface is underlain by permafrost, which contains 25-50% of the total global soil carbon pool (Schuur et al. 2008, Tarnocai et al. 2009). This carbon is largely protected from microbial decomposition by reduced microbial activity in frozen conditions, but climate change is threatening to induce large-scale permafrost thaw thus exposing it to degradation. The resulting emissions of greenhouse gasses (GHGs) can produce a positive feedback loop and significantlymore » amplify the effects of global warming. Increasing temperatures at high latitudes, changes in precipitation patterns, and frequent fire events have already initiated a widespread degradation of permafrost (Schuur et al. 2015).« less

Training effect of the exchange bias in sputter deposited Fe3O4 thin films with varying thickness

NASA Astrophysics Data System (ADS)

Muhammed Shameem, P. V.; Senthil Kumar, M.

2018-07-01

The training effect property of the exchange bias in the reactively sputtered polycrystalline Fe3O4 thin films of varying thicknesses in the range 25-200 nm are studied. Structural studies by X-ray diffraction, X-ray photoelectron spectroscopy and selected area electron diffraction confirm the formation of single phase Fe3O4. The scanning electron spectroscopy images show that the grains are uniformly distributed. All the samples show clear and consistent exchange bias training behaviour due to the dynamics of the spins at the interface of the ferrimagnetic core and the spin glass-like surface of the grains. The analysis of the training effect data of the exchange bias field HE measured at 2 K by using three different models show that the model based on the relaxation of the frozen and rotatable spin components at the interface gives the best description for all the samples. From this model, it is found that the reversible interface spins relax around 7 times faster than the frozen interface spins at 2 K for all the samples and that their relative relaxation rates are independent of the sample thickness. This constancy show that the relative relaxation rates of the interfacial frozen and rotatable spin components is a material dependent property. The frozen component of the interfacial spins of each sample is found to be dominated at the initial stage of the training. A direct equivalence between the HE and remanence asymmetry ME is observed. Above the spin freezing temperature, the training effect measurements at 75 K show that the HE decreases sharply with successive field cycling as compared to the measurements made at 2 K and the HE vanishes after first few cycles.

Hydrocarbon gases associated with permafrost in the Mackenzie Delta, Northwest Territories, Canada

USGS Publications Warehouse

Collett, T.S.; Dallimore, S.R.

1999-01-01

Molecular and isotopic analyses of core gas samples from 3 permafrost research core holes (92GSCTAGLU, 92GSCKUMAK, 92GSCUNIPKAT; sample core depths ranging from 0.36 to 413.82 m) in the Mackenzie Delta of the Northwest Territories of Canada reveal the presence of hydrocarbon gases from both microbial and thermogenic sources. Analyses of most headspace and blended gas samples from the ice-bonded permafrost portion of the core holes yielded C1/(C2 + C3) hydrocarbon gas ratios and CH4-C isotopic compositions (??13C CH4) indicative of microbially sourced CH4 gas. However, near the base of ice-bonded permafrost and into the underlying non-frozen stratigraphic section, an increase in ethane (C2) concentrations, decreases in C1/(C2 + C3) hydrocarbon gas ratios, and CH4-C isotopic (??13C CH4) data indicate the presence of hydrocarbon gases derived from a thermogenic source. The thermogenic gas below permafrost in the Mackenzie Delta likely migrated from deeper hydrocarbon accumulations and/or directly from thermally mature hydrocarbon source rocks.

Memory effect versus exchange bias for maghemite nanoparticles

NASA Astrophysics Data System (ADS)

Nadeem, K.; Krenn, H.; Szabó, D. V.

2015-11-01

We studied the temperature dependence of memory and exchange bias effects and their dependence on each other in maghemite (γ-Fe2O3) nanoparticles by using magnetization studies. Memory effect in zero field cooled process in nanoparticles is a fingerprint of spin-glass behavior which can be due to i) surface disordered spins (surface spin-glass) and/or ii) randomly frozen and interacting nanoparticles core spins (super spin-glass). Temperature region (25-70 K) for measurements has been chosen just below the average blocking temperature (TB=75 K) of the nanoparticles. Memory effect (ME) shows a non-monotonous behavior with temperature. It shows a decreasing trend with decreasing temperature and nearly vanishes below 30 K. However it also decreased again near the blocking temperature of the nanoparticles e.g., 70 K. Exchange bias (EB) in these nanoparticles arises due to core/shell interface interactions. The EB increases sharply below 30 K due to increase in core/shell interactions, while ME starts vanishing below 30 K. We conclude that the core/shell interface interactions or EB have not enhanced the ME but may reduce it in these nanoparticles.

Multiple Restart Testing of a Stainless Steel Sodium Heat Pipe Module

NASA Astrophysics Data System (ADS)

Martin, James; Mireles, Omar; Reid, Robert

2005-02-01

A heat pipe cooled reactor is one of several candidate reactor concepts being considered for space power and propulsion systems to support future space exploration activities. Long life heat pipe modules, with concepts verified through a combination of theoretical analysis and experimental evaluations, would be necessary to establish the viability of this option. A number of stainless steel/sodium heat pipe modules have been designed and fabricated to support experimental testing of a Safe Affordable Fission Engine (SAFE) project, a 100-kWt core design pursued jointly by the Marshall Space Flight Center and the Los Alamos National Laboratory. One of the SAFE heat pipe modules was successfully subjected to over 200 restarts, examining the behavior of multiple passive freeze/thaw operations. Typical operation included a 1-hour startup to an average evaporator temperature of 1000 K followed by a 15-minute hold at temperature. Nominal maximum input power to the evaporator (measured at the power supply) during the hold period was 1.9 kW, with approximately 1.6 kW calculated as the axial power transfer to the condenser (the 300W difference was lost to environment at the evaporator surface). Between heating cycles the module was cooled to less than 325 K, returning the sodium to a frozen state in preparation for the next startup cycle.

Aerosol-Cloud Interactions during Tropical Deep Convection: Evidence for the Importance of Free Tropospheric Aerosols

NASA Technical Reports Server (NTRS)

Ackerman, A.; Jensen, E.; Stevens, D.; Wang, D.; Heymsfield, A.; Miloshevich, L.; Twohy, C.; Poellot, M.; VanReken, T.; Fridland, Ann

2003-01-01

NASA's 2002 CRYSTAL-FACE field experiment focused on the formation and evolution of tropical cirrus cloud systems in southern Florida. Multiple aircraft extensively sampled cumulonimbus dynamical and microphysical properties, as well as characterizing ambient aerosol populations both inside and outside the full depth of the convective column. On July 18, unique measurements were taken when a powerful updraft was traversed directly by aircraft, providing a window into the primary source region of cumulonimbus anvil crystals. Observations of the updraft, entered at approximately l0 km altitude and -34 C, indicated more than 200 cloud particles per mL at vertical velocities exceeding 20 m/s and the presence of significant condensation nuclei and liquid water within the core. In this work, aerosol and cloud phase observations are integrated by simulating the updraft conditions using a large-eddy resolving model with 3 explicit multiphase microphysics, including treatment of size-resolved aerosol fields, aerosol activation and freezing, and evaporation of cloud particles back to the aerosol phase. Simulations were initialized with observed thermodynamic and aerosol size distributions profiles and convection was driven by surface fluxes assimilated from the ARPS forecast model. Model results are consistent with the conclusions that most crystals are homogeneously frozen droplets and that entrained free tropospheric aerosols may contribute a significant fraction of the crystals. Thus most anvil crystals appear to be formed aloft in updraft cores, well above cloud base. These conclusions are supported by observations of hydrometeor size distribution made while traversing the dore, as well as aerosol and cloud particle size distributions generally observed by aircraft below 4km and crystal properties generally observed by aircraft above 12km.

Light domain walls, massive neutrinos and the large scale structure of the Universe

NASA Technical Reports Server (NTRS)

Massarotti, Alessandro

1991-01-01

Domain walls generated through a cosmological phase transition are considered, which interact nongravitationally with light neutrinos. At a redshift z greater than or equal to 10(exp 4), the network grows rapidly and is virtually decoupled from the matter. As the friction with the matter becomes dominant, a comoving network scale close to that of the comoving horizon scale at z of approximately 10(exp 4) gets frozen. During the later phases, the walls produce matter wakes of a thickness d of approximately 10h(exp -1)Mpc, that may become seeds for the formation of the large scale structure observed in the Universe.

ANALYZING NUMERICAL ERRORS IN DOMAIN HEAT TRANSPORT MODELS USING THE CVBEM.

USGS Publications Warehouse

Hromadka, T.V.

1987-01-01

Besides providing an exact solution for steady-state heat conduction processes (Laplace-Poisson equations), the CVBEM (complex variable boundary element method) can be used for the numerical error analysis of domain model solutions. For problems where soil-water phase change latent heat effects dominate the thermal regime, heat transport can be approximately modeled as a time-stepped steady-state condition in the thawed and frozen regions, respectively. The CVBEM provides an exact solution of the two-dimensional steady-state heat transport problem, and also provides the error in matching the prescribed boundary conditions by the development of a modeling error distribution or an approximate boundary generation.

Alfven seismic vibrations of crustal solid-state plasma in quaking paramagnetic neutron star

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bastrukov, S.; Xu, R.-X.; Molodtsova, I.

2010-11-15

Magneto-solid-mechanical model of two-component, core-crust, paramagnetic neutron star responding to quake-induced perturbation by differentially rotational, torsional, oscillations of crustal electron-nuclear solid-state plasma about axis of magnetic field frozen in the immobile paramagnetic core is developed. Particular attention is given to the node-free torsional crust-against-core vibrations under combined action of Lorentz magnetic and Hooke's elastic forces; the damping is attributed to Newtonian force of shear viscose stresses in crustal solid-state plasma. The spectral formulas for the frequency and lifetime of this toroidal mode are derived in analytic form and discussed in the context of quasiperiodic oscillations of the x-ray outburst fluxmore » from quaking magnetars. The application of obtained theoretical spectra to modal analysis of available data on frequencies of oscillating outburst emission suggests that detected variability is the manifestation of crustal Alfven's seismic vibrations restored by Lorentz force of magnetic field stresses.« less

Ice Core Records of West Greenland Melt and Climate Forcing

NASA Astrophysics Data System (ADS)

Graeter, K. A.; Osterberg, E. C.; Ferris, D. G.; Hawley, R. L.; Marshall, H. P.; Lewis, G.; Meehan, T.; McCarthy, F.; Overly, T.; Birkel, S. D.

2018-04-01

Remote sensing observations and climate models indicate that the Greenland Ice Sheet (GrIS) has been losing mass since the late 1990s, mostly due to enhanced surface melting from rising summer temperatures. However, in situ observational records of GrIS melt rates over recent decades are rare. Here we develop a record of frozen meltwater in the west GrIS percolation zone preserved in seven firn cores. Quantifying ice layer distribution as a melt feature percentage (MFP), we find significant increases in MFP in the southernmost five cores over the past 50 years to unprecedented modern levels (since 1550 CE). Annual to decadal changes in summer temperatures and MFP are closely tied to changes in Greenland summer blocking activity and North Atlantic sea surface temperatures since 1870. However, summer warming of 1.2°C since 1870-1900, in addition to warming attributable to recent sea surface temperature and blocking variability, is a critical driver of high modern MFP levels.

A converged calculation of the energy barrier to internal rotation in the ethylene-sulfur dioxide dimer

NASA Astrophysics Data System (ADS)

Resende, Stella M.; De Almeida, Wagner B.; van Duijneveldt-van de Rijdt, Jeanne G. C. M.; van Duijneveldt, Frans B.

2001-08-01

Geometrical parameters for the equilibrium (MIN) and lowest saddle-point (TS) geometries of the C2H4⋯SO2 dimer, and the corresponding binding energies, were calculated using the Hartree-Fock and correlated levels of ab initio theory, in basis sets ranging from the D95(d,p) double-zeta basis set to the aug-cc-pVQZ correlation consistent basis set. An assessment of the effect of the basis set superposition error (BSSE) on these results was made. The dissociation energy from the lowest vibrational state was estimated to be 705±100 cm-1 at the basis set limit, which is well within the range expected from experiment. The barrier to internal rotation was found to be 53±5 cm-1, slightly higher than the (revised) experimental result of 43 cm-1, probably due to zero-point vibrational effects. Our results clearly show that, in direct contrast with recent ideas, the BSSE correction affects differentially the MIN and TS binding energies and so has to be included in the calculation of small energy barriers such as that in the C2H4⋯SO2 dimer. Previous reports of positive MP2 frozen-core binding energies for this complex in basis D95(d,p) are confirmed. The anomalies are shown to be an artifact arising from an incorrect removal of virtual orbitals by the default frozen-core option in the GAUSSIAN program.

Cryopreservation of spermatozoa of black marlin, Makaira indica (Teleostei: Istiophoridae).

PubMed

van der Straten, K M; Leung, L K-P; Rossini, R; Johnston, S D

2006-01-01

As a first step towards the development of a method for the cryopreservation of black marlin spermatozoa, this study investigated the effect of dimethylsulfoxide (DMSO) concentration and pellet size on post-thaw spermatozoal motility. Spermatozoa were recovered from the spermatic duct of testes retrieved post-mortem from four adult black marlin caught in the Coral Sea spawning grounds of Australia. Undiluted spermatozoa were stored on ice for 4 to 10 hours during transport to shore, then evaluated for motility after activation in seawater (1:10 v:v). Spermatozoa were prepared for cryopreservation in pellets by extension (1:3 v:v) in a defined fish Ringer's solution to give two final DMSO concentrations of 2.5% or 5.0%. Diluted spermatozoa were frozen directly on a dry ice block in pellet sizes of either 0.25 ml or 0.50 ml. Frozen pellets were thawed in a water bath at 40 degrees C for 60 seconds and assessed for post-thaw motility following activation in seawater. Spermatozoa recovered within 50 minutes of death and chilled on ice for 4 to 10 hours showed a mean (+/- SEM) motility immediately following activation of 91.6 +/- 7.9%. 50% of the spermatozoa remained motile for approximately 4 to 5 minutes. Following cryopreservation, mean motility declined significantly across all cryoprotectant and pellet size combinations (P < 0.001) but spermatozoa frozen in 2.5% DMSO showed higher motility than those frozen in 5.0% DMSO (P = 0.014). Pellet size had no effect on post-thaw motility (P = 0.179).

DNA methylation profiling of genomic DNA isolated from urine in diabetic chronic kidney disease: A pilot study

PubMed Central

Sexton-Oates, Alexandra; Carmody, Jake; Ekinci, Elif I.; Dwyer, Karen M.; Saffery, Richard

2018-01-01

Aim To characterise the genomic DNA (gDNA) yield from urine and quality of derived methylation data generated from the widely used Illuminia Infinium MethylationEPIC (HM850K) platform and compare this with buffy coat samples. Background DNA methylation is the most widely studied epigenetic mark and variations in DNA methylation profile have been implicated in diabetes which affects approximately 415 million people worldwide. Methods QIAamp Viral RNA Mini Kit and QIAamp DNA micro kit were used to extract DNA from frozen and fresh urine samples as well as increasing volumes of fresh urine. Matched buffy coats to the frozen urine were also obtained and DNA was extracted from the buffy coats using the QIAamp DNA Mini Kit. Genomic DNA of greater concentration than 20μg/ml were used for methylation analysis using the HM850K array. Results Irrespective of extraction technique or the use of fresh versus frozen urine samples, limited genomic DNA was obtained using a starting sample volume of 5ml (0–0.86μg/mL). In order to optimize the yield, we increased starting volumes to 50ml fresh urine, which yielded only 0–9.66μg/mL A different kit, QIAamp DNA Micro Kit, was trialled in six fresh urine samples and ten frozen urine samples with inadequate DNA yields from 0–17.7μg/mL and 0–1.6μg/mL respectively. Sufficient genomic DNA was obtained from only 4 of the initial 41 frozen urine samples (10%) for DNA methylation profiling. In comparison, all four buffy coat samples (100%) provided sufficient genomic DNA. Conclusion High quality data can be obtained provided a sufficient yield of genomic DNA is isolated. Despite optimizing various extraction methodologies, the modest amount of genomic DNA derived from urine, may limit the generalisability of this approach for the identification of DNA methylation biomarkers of chronic diabetic kidney disease. PMID:29462136

Calcium chloride: A new solution for frozen coal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boley, D.G.

Few situations can strike more terror into the hearts of utility and industrial powerplant managers than the arrival of hopper cars loaded with solidly frozen coal. If the cars aren't emptied and returned to the railroad, usually within about 48 hours, demurrage (the equipment-detention charge) begins and can quickly rise to $50 or more per day per car. All to frequently, the hasty solution is to assign $16/hr workers the task of manually breaking up the frozen coal, using techniques that the mining industry considered obsolete 50 years ago. A CaCl/sub 2/ system represents a small investment. Either in drymore » or liquid form, the chemical is inexpensive and is consumed only when it is needed. The essential equipment, which is easy to operate and maintain, consists primarily of a storage tank, a pump, the necessary piping or hose, and either a fixed or portable spray applicator. A flowmeter will monitor the application rate, and the tank may be optionally heated. Application cost, including labor, for CaCl/sub 2/ is usually between $2.65 and $3.25 per treated ton. This is approximately half the cost of energy, per ton of coal, consumed by a thaw shed. In an emergency, when railcar demurrage costs are building at $50 or more per day per car, CaCl/sub 2/ is not only an inexpensive solution; it is possibly the only practical answer to the frozen-coal problem (see box, below). When used by itself, CaCl/sub 2/ minimizes the cost and frustration of unloading frozen coal because labor, equipment, chemical, energy, and maintenance costs are all held in tight control. When used to complement mechanical and/or thermal techniques, it increases unloading capacity, thereby improving productivity and helping to control all costs of cold-weather coal handling.« less

DNA methylation profiling of genomic DNA isolated from urine in diabetic chronic kidney disease: A pilot study.

PubMed

Lecamwasam, Ashani; Sexton-Oates, Alexandra; Carmody, Jake; Ekinci, Elif I; Dwyer, Karen M; Saffery, Richard

2018-01-01

To characterise the genomic DNA (gDNA) yield from urine and quality of derived methylation data generated from the widely used Illuminia Infinium MethylationEPIC (HM850K) platform and compare this with buffy coat samples. DNA methylation is the most widely studied epigenetic mark and variations in DNA methylation profile have been implicated in diabetes which affects approximately 415 million people worldwide. QIAamp Viral RNA Mini Kit and QIAamp DNA micro kit were used to extract DNA from frozen and fresh urine samples as well as increasing volumes of fresh urine. Matched buffy coats to the frozen urine were also obtained and DNA was extracted from the buffy coats using the QIAamp DNA Mini Kit. Genomic DNA of greater concentration than 20μg/ml were used for methylation analysis using the HM850K array. Irrespective of extraction technique or the use of fresh versus frozen urine samples, limited genomic DNA was obtained using a starting sample volume of 5ml (0-0.86μg/mL). In order to optimize the yield, we increased starting volumes to 50ml fresh urine, which yielded only 0-9.66μg/mL A different kit, QIAamp DNA Micro Kit, was trialled in six fresh urine samples and ten frozen urine samples with inadequate DNA yields from 0-17.7μg/mL and 0-1.6μg/mL respectively. Sufficient genomic DNA was obtained from only 4 of the initial 41 frozen urine samples (10%) for DNA methylation profiling. In comparison, all four buffy coat samples (100%) provided sufficient genomic DNA. High quality data can be obtained provided a sufficient yield of genomic DNA is isolated. Despite optimizing various extraction methodologies, the modest amount of genomic DNA derived from urine, may limit the generalisability of this approach for the identification of DNA methylation biomarkers of chronic diabetic kidney disease.

Computer program for calculation of complex chemical equilibrium compositions and applications. Supplement 1: Transport properties

NASA Technical Reports Server (NTRS)

Gordon, S.; Mcbride, B.; Zeleznik, F. J.

1984-01-01

An addition to the computer program of NASA SP-273 is given that permits transport property calculations for the gaseous phase. Approximate mixture formulas are used to obtain viscosity and frozen thermal conductivity. Reaction thermal conductivity is obtained by the same method as in NASA TN D-7056. Transport properties for 154 gaseous species were selected for use with the program.

Publications - GMC 310 | Alaska Division of Geological & Geophysical

Science.gov Websites

approximations of core (4,309.5'-4,409') from the BP Exploration (Alaska) Inc. Milne Point G-1 well Authors UCS approximations of core (4,309.5'-4,409') from the BP Exploration (Alaska) Inc. Milne Point G-1

A sample-freezing drive shoe for a wire line piston core sampler

USGS Publications Warehouse

Murphy, F.; Herkelrath, W.N.

1996-01-01

Loss of fluids and samples during retrieval of cores of saturated, noncohesive sediments results in incorrect measures of fluid distributions and an inaccurate measure of the stratigraphic position of the sample. To reduce these errors, we developed a hollow drive shoe that freezes in place the lowest 3 inches (75 mm) of a 1.88-inch-diameter (48 mm), 5-foot-long (1.5 m) sediment sample taken using a commercial wire line piston core sampler. The end of the core is frozen by piping liquid carbon dioxide at ambient temperature through a steel tube from a bottle at the land surface to the drive shoe where it evaporates and expands, cooling the interior surface of the shoe to about -109??F (-78??C). Freezing a core end takes about 10 minutes. The device was used to collect samples for a study of oil-water-air distributions, and for studies of water chemistry and microbial activity in unconsolidated sediments at the site of an oil spill near Bemidji, Minnesota. Before freezing was employed, samples of sandy sediments from near the water table sometimes flowed out of the core barrel as the sampler was withdrawn. Freezing the bottom of the core allowed for the retention of all material that entered the core barrel and lessened the redistribution of fluids within the core. The device is useful in the unsaturated and shallow saturated zones, but does not freeze cores well at depths greater than about 20 feet (6 m) below water, possibly because the feed tube plugs with dry ice with increased exhaust back-pressure, or because sediment enters the annulus between the core barrel and the core barrel liner and blocks the exhaust.

Properties of gamma-ray burst progenitor stars.

PubMed

Kumar, Pawan; Narayan, Ramesh; Johnson, Jarrett L

2008-07-18

We determined some basic properties of stars that produce spectacular gamma-ray bursts at the end of their lives. We assumed that accretion of the outer portion of the stellar core by a central black hole fuels the prompt emission and that fall-back and accretion of the stellar envelope later produce the plateau in the x-ray light curve seen in some bursts. Using x-ray data for three bursts, we estimated the radius of the stellar core to be approximately (1 - 3) x 10(10) cm and that of the stellar envelope to be approximately (1 - 2) x 10(11) cm. The density profile in the envelope is fairly shallow, with rho approximately r(-2) (where rho is density and r is distance from the center of the explosion). The rotation speeds of the core and envelope are approximately 0.05 and approximately 0.2 of the local Keplerian speed, respectively.

Limited contribution of permafrost carbon to methane release from thawing peatlands

NASA Astrophysics Data System (ADS)

Cooper, Mark D. A.; Estop-Aragonés, Cristian; Fisher, James P.; Thierry, Aaron; Garnett, Mark H.; Charman, Dan J.; Murton, Julian B.; Phoenix, Gareth K.; Treharne, Rachael; Kokelj, Steve V.; Wolfe, Stephen A.; Lewkowicz, Antoni G.; Williams, Mathew; Hartley, Iain P.

2017-07-01

Models predict that thaw of permafrost soils at northern high latitudes will release tens of billions of tonnes of carbon (C) to the atmosphere by 2100 (refs ,,). The effect on the Earth’s climate depends strongly on the proportion of this C that is released as the more powerful greenhouse gas methane (CH4), rather than carbon dioxide (CO2) (refs ,); even if CH4 emissions represent just 2% of the C release, they would contribute approximately one-quarter of the climate forcing. In northern peatlands, thaw of ice-rich permafrost causes surface subsidence (thermokarst) and water-logging, exposing substantial stores (tens of kilograms of C per square meter, ref. ) of previously frozen organic matter to anaerobic conditions, and generating ideal conditions for permafrost-derived CH4 release. Here we show that, contrary to expectations, although substantial CH4 fluxes (>20 g CH4 m-2 yr-1) were recorded from thawing peatlands in northern Canada, only a small amount was derived from previously frozen C (<2 g CH4 m-2 yr-1). Instead, fluxes were driven by anaerobic decomposition of recent C inputs. We conclude that thaw-induced changes in surface wetness and wetland area, rather than the anaerobic decomposition of previously frozen C, may determine the effect of permafrost thaw on CH4 emissions from northern peatlands.

Electronically nonadiabatic wave packet propagation using frozen Gaussian scattering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kondorskiy, Alexey D., E-mail: kondor@sci.lebedev.ru; Nanbu, Shinkoh, E-mail: shinkoh.nanbu@sophia.ac.jp

2015-09-21

We present an approach, which allows to employ the adiabatic wave packet propagation technique and semiclassical theory to treat the nonadiabatic processes by using trajectory hopping. The approach developed generates a bunch of hopping trajectories and gives all additional information to incorporate the effect of nonadiabatic coupling into the wave packet dynamics. This provides an interface between a general adiabatic frozen Gaussian wave packet propagation method and the trajectory surface hopping technique. The basic idea suggested in [A. D. Kondorskiy and H. Nakamura, J. Chem. Phys. 120, 8937 (2004)] is revisited and complemented in the present work by the elaborationmore » of efficient numerical algorithms. We combine our approach with the adiabatic Herman-Kluk frozen Gaussian approximation. The efficiency and accuracy of the resulting method is demonstrated by applying it to popular benchmark model systems including three Tully’s models and 24D model of pyrazine. It is shown that photoabsorption spectrum is successfully reproduced by using a few hundreds of trajectories. We employ the compact finite difference Hessian update scheme to consider feasibility of the ab initio “on-the-fly” simulations. It is found that this technique allows us to obtain the reliable final results using several Hessian matrix calculations per trajectory.« less

78 FR 2946 - United States Standards for Grades of Frozen Vegetables

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-15

... these proposed revisions are: frozen asparagus, frozen lima beans, frozen speckled butter beans, frozen... document. United States Standards for Grades of Frozen Lima Beans Update address for AMS. Change ``U.S... obtain color standards for frozen lima beans. United States Standards for Grades of Frozen Mixed...

Molecular Composition and Chemistry of Isolated Dense Cores

NASA Astrophysics Data System (ADS)

Cook, Amanda; Boogert, A.

2009-01-01

The composition of molecular clouds and the envelopes and disks surrounding low mass protostars within them is still poorly known. There is little doubt that a large fraction of the molecules is frozen on grains, but the abundance of several crucial species (e.g. ammonia, methanol, ions) in the ices is still uncertain. In addition, prominent spectral features discovered decades ago are still not securely identified (e.g. the 6.85-micron absorption band). Gas phase and grain surface chemistry play pivotal roles in molecule formation, but numerous other processes could have significant impacts as well: shocks, thermal heating, irradiation of ices by ultraviolet photons and cosmic rays. Complex species could be formed this way, profoundly influencing cloud, disk and planetary/cometary chemistry. We have obtained Spitzer/IRS spectra of an unprecedented sample of sight-lines tracing 25 dense isolated cores. These cores physically differ from the large, cluster-forming molecular clouds (e.g. Ophiuchus, Perseus) that are commonly studied: they are less turbulent, colder, less dense, and likely longer lived. These IRS spectra of isolated cores thus provide unique information on ice formation and destruction mechanisms. Toward the same cores, we observed 33 highly extincted background stars as well, tracing the quiescent cloud medium against which the ices around protostars can be contrasted.

Efficient time-dependent density functional theory approximations for hybrid density functionals: analytical gradients and parallelization.

PubMed

Petrenko, Taras; Kossmann, Simone; Neese, Frank

2011-02-07

In this paper, we present the implementation of efficient approximations to time-dependent density functional theory (TDDFT) within the Tamm-Dancoff approximation (TDA) for hybrid density functionals. For the calculation of the TDDFT/TDA excitation energies and analytical gradients, we combine the resolution of identity (RI-J) algorithm for the computation of the Coulomb terms and the recently introduced "chain of spheres exchange" (COSX) algorithm for the calculation of the exchange terms. It is shown that for extended basis sets, the RIJCOSX approximation leads to speedups of up to 2 orders of magnitude compared to traditional methods, as demonstrated for hydrocarbon chains. The accuracy of the adiabatic transition energies, excited state structures, and vibrational frequencies is assessed on a set of 27 excited states for 25 molecules with the configuration interaction singles and hybrid TDDFT/TDA methods using various basis sets. Compared to the canonical values, the typical error in transition energies is of the order of 0.01 eV. Similar to the ground-state results, excited state equilibrium geometries differ by less than 0.3 pm in the bond distances and 0.5° in the bond angles from the canonical values. The typical error in the calculated excited state normal coordinate displacements is of the order of 0.01, and relative error in the calculated excited state vibrational frequencies is less than 1%. The errors introduced by the RIJCOSX approximation are, thus, insignificant compared to the errors related to the approximate nature of the TDDFT methods and basis set truncation. For TDDFT/TDA energy and gradient calculations on Ag-TB2-helicate (156 atoms, 2732 basis functions), it is demonstrated that the COSX algorithm parallelizes almost perfectly (speedup ~26-29 for 30 processors). The exchange-correlation terms also parallelize well (speedup ~27-29 for 30 processors). The solution of the Z-vector equations shows a speedup of ~24 on 30 processors. The parallelization efficiency for the Coulomb terms can be somewhat smaller (speedup ~15-25 for 30 processors), but their contribution to the total calculation time is small. Thus, the parallel program completes a Becke3-Lee-Yang-Parr energy and gradient calculation on the Ag-TB2-helicate in less than 4 h on 30 processors. We also present the necessary extension of the Lagrangian formalism, which enables the calculation of the TDDFT excited state properties in the frozen-core approximation. The algorithms described in this work are implemented into the ORCA electronic structure system.

Computational Prediction of Kinetic Rate Constants

DTIC Science & Technology

2006-06-09

24 Reactant: P(CH3)F2O (Difluor) Energetics: RHF DZP Frozen Core CCSD: -655.069925 Hartree CCSD(T): -655.090871 Hartree ZPE : 30.59 kcal...655.069925 Hartree CCSD(T): -655.090871 Hartree ZPE : 30.59 kcal/mol Geometry: RHF CCSD(T) Geom. Opt. PC Bond (Å): 1.79575 OP Bond (Å): 1.47117 FP Bond (Å... ZPE : 18.67 kcal/mol Intensity (km/mol)Frequency (cm -1)Symmetry 11.03833324.0968A1 11.03833324.0968A1 0.00003133.6602A1 2.38681429.9058A1

Onset of the Magnetic Explosion in Filament-Eruption Flares and Coronal Mass Ejections: Single-Bipole Events

NASA Technical Reports Server (NTRS)

Moore, Ron L.; Sterling, Alphonse C.

2000-01-01

We present three-dimensional sketches of die magnetic field before and during filament eruptions in flares and coronal mass ejections. Before the eruption, the overall magnetic field is a closed bipole in which the core field (the field rooted along the bipole's neutral line in the photospheric magnetic flux) is strongly sheared and has oppositely curved "elbows" that bulge out from the opposite ends of the neutral line. This core-field sigmoid runs under and is pressed down in the middle by the rest of the field in the bipole, the less-sheared envelope field rooted outside the core field (as in the model of Antiochos, Dahlburg, & Klimchuk. A filament of chromospheric-temperature plasma is often held in the core field over the neutral line. In a filament eruption, the core field undergoes an explosive eruption, the frozen-in filament plasma providing a visible tracer of the erupting field. The core-field explosion may be either confined (as in some flares) or ejective (as in CMEs that begin together with the onset of a long-duration two-ribbon flare). We present examples of each of these two kind of events as observed in sequences of coronal X-ray images from the Yohkoh SXT, and consider (1) how the explosion begins, and (2) whether confined eruptions begin in basically the same way as ejective eruptions.

Constraining Genome-Scale Models to Represent the Bow Tie Structure of Metabolism for 13C Metabolic Flux Analysis

PubMed Central

Ando, David; Singh, Jahnavi; Keasling, Jay D.; García Martín, Héctor

2018-01-01

Determination of internal metabolic fluxes is crucial for fundamental and applied biology because they map how carbon and electrons flow through metabolism to enable cell function. 13C Metabolic Flux Analysis (13C MFA) and Two-Scale 13C Metabolic Flux Analysis (2S-13C MFA) are two techniques used to determine such fluxes. Both operate on the simplifying approximation that metabolic flux from peripheral metabolism into central “core” carbon metabolism is minimal, and can be omitted when modeling isotopic labeling in core metabolism. The validity of this “two-scale” or “bow tie” approximation is supported both by the ability to accurately model experimental isotopic labeling data, and by experimentally verified metabolic engineering predictions using these methods. However, the boundaries of core metabolism that satisfy this approximation can vary across species, and across cell culture conditions. Here, we present a set of algorithms that (1) systematically calculate flux bounds for any specified “core” of a genome-scale model so as to satisfy the bow tie approximation and (2) automatically identify an updated set of core reactions that can satisfy this approximation more efficiently. First, we leverage linear programming to simultaneously identify the lowest fluxes from peripheral metabolism into core metabolism compatible with the observed growth rate and extracellular metabolite exchange fluxes. Second, we use Simulated Annealing to identify an updated set of core reactions that allow for a minimum of fluxes into core metabolism to satisfy these experimental constraints. Together, these methods accelerate and automate the identification of a biologically reasonable set of core reactions for use with 13C MFA or 2S-13C MFA, as well as provide for a substantially lower set of flux bounds for fluxes into the core as compared with previous methods. We provide an open source Python implementation of these algorithms at https://github.com/JBEI/limitfluxtocore. PMID:29300340

Relativistic many-body XMCD theory including core degenerate effects

NASA Astrophysics Data System (ADS)

Fujikawa, Takashi

2009-11-01

A many-body relativistic theory to analyze X-ray Magnetic Circular Dichroism (XMCD) spectra has been developed on the basis of relativistic quantum electrodynamic (QED) Keldysh Green's function approach. This theoretical framework enables us to handle relativistic many-body effects in terms of correlated nonrelativistic Green's function and relativistic correction operator Q, which naturally incorporates radiation field screening and other optical field effects in addition to electron-electron interactions. The former can describe the intensity ratio of L2/L3 which deviates from the statistical weight (branching ratio) 1/2. In addition to these effects, we consider the degenerate or nearly degenerate effects of core levels from which photoelectrons are excited. In XPS spectra, for example in Rh 3d sub level excitations, their peak shapes are quite different: This interesting behavior is explained by core-hole moving after the core excitation. We discuss similar problems in X-ray absorption spectra in particular excitation from deep 2p sub levels which are degenerate in each sub levels and nearly degenerate to each other in light elements: The hole left behind is not frozen there. We derive practical multiple scattering formulas which incorporate all those effects.

Psychrophiles and astrobiology: microbial life of frozen worlds

NASA Astrophysics Data System (ADS)

Pikuta, Elena V.; Hoover, Richard B.

2003-01-01

Most bodies of our Solar System are "Frozen Worlds" where the prevailing surface temperature remains at or below freezing. On Earth there are vast permanently frozen regions of permafrost, polar ice sheets, and glaciers and the deep oceans and deep-sea marine sediments have remained at 2 - 4°C for eons. Psychrophilic and psychrotrophic microbiota that inhabit these regimes provide analogs for microbial life that might inhabit ice sheets and permafrost of Mars, comets, or the ice/water interfaces or sediments deep beneath the icy crusts of Europa, Callisto, or Ganymede. Cryopreserved micro-organisms can remain viable (in a deep anabiotic state) for millions of years frozen in permafrost and ice. Psychrophilic and psychrotrophic (cold-loving) microbes can carry out metabolic processes in water films and brine, acidic, or alkaline chanels in permafrost or ice at temperatures far below 0°C. These microbes of the cryosphere help define the thermal and temporal limits of life on Earth and may provide clues to where and how to search for evidence of life elsewhere in the Cosmos. Astrobiologists at the NASA Marshall Space Flight Center have collected microbial extremophiles from the Pleistocene ice wedges and frozen thermokarst ponds from the Fox Permafrost Tunnel of Alaska. Microbes have also been isolated from samples of Magellanic Penguin guano from Patagonia; deep-sea marine muds near hydrothermal vents; snow and permafrost from Siberia, and deep ice cores, ice-bubble and cryoconite rocks of the Central Antarctic Ice Sheet. These samples have yielded microbial extremophiles representing a wide variety of anaerobic bacteria and archaea. These microbes have been isolated, cultured, characterized and analyzed by phylogenetic and genomic methods. Images were obtained by Phase Contrast, Environmental, Field Emission Scanning and Transmission Electron Microscopes to study the ultra-microstructure and elemental distribution in the composition of these micro-organisms. We consider the Astrobiological significance of the Fox Tunnel with its rich assemblage of frozen microbes as proxy for developing techniques that may help optimize the search for evidence of life in the permafrost of Mars. We provide images of a novel anaerobic, heterotrophic, psychrotrophic bacterium (str.FTR1) isolated in pure culture from the Fox Tunnel. We also describe novel psychrotrophs isolated from guano of the Magellanic penguin (Spheniscus magellanicus) from the southern tip of Patagonia. These strains PmagG1 and PPP2) represent new species and genera of anaerobic microbes that grow at very low temperatures. The lowest limit for growth without morphological changes of str.PmagG1 is -4°C.

A high-resolution land model coupled with groundwater lateral flow, human water regulation and the changes in soil freeze-thaw fronts

NASA Astrophysics Data System (ADS)

Xie, Z.; Zeng, Y.; Liu, S.; Gao, J.; Jia, B.; Qin, P.

2017-12-01

Both anthropogenic water regulation and groundwater lateral flow essentially affect groundwater table patterns. Their relationship is close because lateral flow recharges the groundwater depletion cone, which is induced by over-exploitation. And the movement of frost and thaw fronts (FTFs) affects soil water and thermal characteristics, as well as energy and water exchanges between land surface and the atmosphere. In this study, schemes describing groundwater lateral flow, human water regulation and the changes in soil freeze-thaw fronts were developed and incorporated into the Community Land Model 4.5. Then the model was applied in Heihe River Basin(HRB), an arid and semiarid region, northwest China. High resolution ( 1 km) numerical simulations showed that groundwater lateral flow driven by changes in water heads can essentially change the groundwater table pattern with the deeper water table appearing in the hillslope regions and shallower water table appearing in valley bottom regions and plains. Over the last decade, anthropogenic groundwater exploitation deepened the water table by approximately 2 m in the middle reaches of the HRB and rapidly reduced the terrestrial water storage, while irrigation increased soil moisture by approximately 0.1 m3 m-3. The water stored in the mainstream of the Heihe River was also reduced by human surface water withdrawal. The latent heat flux was increased by 30 W m-2 over the irrigated region, with an identical decrease in sensible heat flux. The simulated groundwater lateral flow was shown to effectively recharge the groundwater depletion cone caused by over-exploitation. The offset rate is higher in plains than mountainous regions. In addition, the simulated FTFs depth compared well with the observed data both in D66 station (permafrost) and Hulugou station (seasonally frozen ground). Over the HRB, the upstream area is permafrost region with maximum thawed depth at 2.5 m and lower region is seasonal frozen ground region with maximum frozen depth at 3 m.

Life in Ice: Microbial Growth Dynamics and Greenhouse Gas Production During Winter in a Thermokarst Bog Revealed by Stable Isotope Probing Targeted Metagenomics

NASA Astrophysics Data System (ADS)

Blazewicz, S.; White, R. A., III; Tas, N.; Euskirchen, E. S.; Mcfarland, J. W.; Jansson, J.; Waldrop, M. P.

2016-12-01

Permafrost contains a reservoir of frozen C estimated to be twice the size of the current atmospheric C pool. In response to changing climate, permafrost is rapidly warming which could result in widespread seasonal thawing. When permafrost thaws, soils that are rich in ice and C often transform into thermokarst wetlands with anaerobic conditions and significant production of atmospheric CH4. While most C flux research in recently thawed permafrost concentrates on the few summer months when seasonal thaw has occurred, there is mounting evidence that sizeable portions of annual CO2 and CH4 efflux occurs over winter or during a rapid burst of emissions associated with seasonal thaw. A potential mechanism for such efflux patterns is microbial activity in frozen soils over winter where gasses produced are partially trapped within ice until spring thaw. In order to better understand microbial transformation of soil C to greenhouse gas over winter, we applied stable isotope probing (SIP) targeted metagenomics combined with process measurements and field flux data to reveal activities of microbial communities in `frozen' soil from an Alaskan thermokarst bog. Field studies revealed build-up of CO2 and CH4 in frozen soils suggesting that microbial activity persisted throughout the winter in soils poised just below the freezing point. Laboratory incubations designed to simulate in-situ winter conditions (-1.5 °C and anaerobic) revealed continuous CH4 and CO2 production. Strikingly, the quantity of CH4 produced in 6 months in frozen soil was equivalent to approximately 80% of CH4 emitted during the 3 month summer `active' season. Heavy water SIP targeted iTag sequencing revealed growing bacteria and archaea in the frozen anaerobic soil. Growth was primarily observed in two bacterial phyla, Firmicutes and Bacteroidetes, suggesting that fermentation was likely the major C mineralization pathway. SIP targeted metagenomics facilitated characterization of the primary metabolic pathways in growing organisms that likely drove C mineralization. Results indicate that winter microbial activities can play an important role in controlling seasonal C flux in recent thawed permafrost and characterization of growing organisms leads to stronger mechanistic linkages between the soil microbial community and ecosystem processes.

Comparison of fertility results after vaginal insemination using different thawing procedures and packages for frozen ram semen

PubMed Central

Paulenz, Heiko; Ådnøy, Tormod; Söderquist, Lennart

2007-01-01

Background The effect of different thawing procedures for ram semen frozen in minitubes and mini straws on the fertility of sheep was tested in a field trial. Methods Altogether, 719 Norwegian Crossbred ewes, aged between six months and six-and-a-half years from 8 farms, were inseminated vaginally in natural oestrus with frozen-thawed semen. Minitubes were thawed at 70°C for 8 sec (T70) and mini straws either at 50°C for 9 sec (S50) or at 35°C for 12 sec (S35). Results Vaginal insemination with 200 × 106 spermatozoa resulted in 25-days non-return rates of 63.2, 59.6, and 62.5% (overall 61.8%), respectively, and lambing rates of 56.8, 55.0, and 59.2% (overall 57.0%), respectively. No significant effect on fertility (as 25-days non-return- or lambing rate) was seen for straw type/thawing temperature (P = 0.5/0.5), but semen filled in mini straws and thawed at 35°C resulted numerically in the highest lambing rate (59.2%). A significant effect was, however, seen for farmer (P = >0.0001/>0.0001) and ram (P = 0.009/0.002). Moreover, age of the ewes had a significant effect on the NR rate (0.007), but not on lambing rate (P = 0.2). Conclusion A vaginal deposition of frozen ram semen containing approximately 200 × 106 spermatozoa, filled in mini straws and thawed at 35°C is a simplified technique that under field conditions and used on a do-it-yourself regime gives acceptable lambing rates in Norway. PMID:17903246

Breakdown of the Frozen-in Condition and Plasma Acceleration: Dynamical Theory

NASA Astrophysics Data System (ADS)

Song, Y.; Lysak, R. L.

2007-12-01

The magnetic reconnection hypothesis emphasizes the importance of the breakdown of the frozen-in condition, explains the strong dependence of the geomagnetic activity on the IMF, and approximates an average qualitative description for many IMF controlled effects in magnetospheric physics. However, some important theoretical aspects of reconnection, including its definition, have not been carefully examined. The crucial components of such models, such as the largely-accepted X-line reconnection picture and the broadly-used explanations of the breakdown of the frozen-in condition, lack complete theoretical support. The important irreversible reactive interaction is intrinsically excluded and overlooked in most reconnection models. The generation of parallel electric fields must be the result of a reactive plasma interaction, which is associated with the temporal changes and spatial gradients of magnetic and velocity shears (Song and Lysak, 2006). Unlike previous descriptions of the magnetic reconnection process, which depend on dissipative-type coefficients or some passive terms in the generalized Ohm's law, the reactive interaction is a dynamical process, which favors localized high magnetic and/or mechanical stresses and a low plasma density. The reactive interaction is often closely associated with the radiation of shear Alfvén waves and is independent of any assumed dissipation coefficients. The generated parallel electric field makes an irreversible conversion between magnetic energy and the kinetic energy of the accelerated plasma and the bulk flow. We demonstrate how the reactive interaction, e.g., the nonlinear interaction of MHD mesoscale wave packets at current sheets and in the auroral acceleration region, can create and support parallel electric fields, causing the breakdown of the frozen-in condition and plasma acceleration.

Drilling Automation Demonstrations in Subsurface Exploration for Astrobiology

NASA Technical Reports Server (NTRS)

Glass, Brian; Cannon, H.; Lee, P.; Hanagud, S.; Davis, K.

2006-01-01

This project proposes to study subsurface permafrost microbial habitats at a relevant Arctic Mars-analog site (Haughton Crater, Devon Island, Canada) while developing and maturing the subsurface drilling and drilling automation technologies that will be required by post-2010 missions. It builds on earlier drilling technology projects to add permafrost and ice-drilling capabilities to 5m with a lightweight drill that will be automatically monitored and controlled in-situ. Frozen cores obtained with this drill under sterilized protocols will be used in testing three hypotheses pertaining to near-surface physical geology and ground H2O ice distribution, viewed as a habitat for microbial life in subsurface ice and ice-consolidated sediments. Automation technologies employed will demonstrate hands-off diagnostics and drill control, using novel vibrational dynamical analysis methods and model-based reasoning to monitor and identify drilling fault states before and during faults. Three field deployments, to a Mars-analog site with frozen impact crater fallback breccia, will support science goals, provide a rigorous test of drilling automation and lightweight permafrost drilling, and leverage past experience with the field site s particular logistics.

Formation and character of an ancient 19-m ice cover and underlying trapped brine in an "ice-sealed" east Antarctic lake.

PubMed

Doran, Peter T; Fritsen, Christian H; McKay, Christopher P; Priscu, John C; Adams, Edward E

2003-01-07

Lake Vida, one of the largest lakes in the McMurdo Dry Valleys of Antarctica, was previously believed to be shallow (<10 m) and frozen to its bed year-round. New ice-core analysis and temperature data show that beneath 19 m of ice is a water column composed of a NaCl brine with a salinity seven times that of seawater that remains liquid below -10 degrees C. The ice cover thickens at both its base and surface, sealing concentrated brine beneath. The ice cover is stabilized by a negative feedback between ice growth and the freezing-point depression of the brine. The ice cover contains frozen microbial mats throughout that are viable after thawing and has a history that extends to at least 2,800 (14)C years B.P., suggesting that the brine has been isolated from the atmosphere for as long. To our knowledge, Lake Vida has the thickest subaerial lake ice cover recorded and may represent a previously undiscovered end-member lacustrine ecosystem on Earth.

Formation and character of an ancient 19-m ice cover and underlying trapped brine in an “ice-sealed” east Antarctic lake

PubMed Central

Doran, Peter T.; Fritsen, Christian H.; McKay, Christopher P.; Priscu, John C.; Adams, Edward E.

2003-01-01

Lake Vida, one of the largest lakes in the McMurdo Dry Valleys of Antarctica, was previously believed to be shallow (<10 m) and frozen to its bed year-round. New ice-core analysis and temperature data show that beneath 19 m of ice is a water column composed of a NaCl brine with a salinity seven times that of seawater that remains liquid below −10°C. The ice cover thickens at both its base and surface, sealing concentrated brine beneath. The ice cover is stabilized by a negative feedback between ice growth and the freezing-point depression of the brine. The ice cover contains frozen microbial mats throughout that are viable after thawing and has a history that extends to at least 2,800 14C years B.P., suggesting that the brine has been isolated from the atmosphere for as long. To our knowledge, Lake Vida has the thickest subaerial lake ice cover recorded and may represent a previously undiscovered end-member lacustrine ecosystem on Earth. PMID:12518052

Random phase approximation and cluster mean field studies of hard core Bose Hubbard model

NASA Astrophysics Data System (ADS)

Alavani, Bhargav K.; Gaude, Pallavi P.; Pai, Ramesh V.

2018-04-01

We investigate zero temperature and finite temperature properties of the Bose Hubbard Model in the hard core limit using Random Phase Approximation (RPA) and Cluster Mean Field Theory (CMFT). We show that our RPA calculations are able to capture quantum and thermal fluctuations significantly better than CMFT.

Determination of the methylation status of MGMT in different regions within glioblastoma multiforme.

PubMed

Hamilton, Mark G; Roldán, Gloria; Magliocco, Anthony; McIntyre, John B; Parney, Ian; Easaw, Jacob C

2011-04-01

Epigenetic silencing of the MGMT gene through promoter methylation correlates with improved survival in Glioblastoma Multiforme (GBM) patients receiving concurrent chemoradiotherapy. Although the clinical benefit is primarily seen in patients with methylated MGMT promoter, some unmethylated patients also respond to Temozolomide. One possible explanation may be intratumoral heterogeneity. This study was designed to assess the methylation status of the MGMT promoter in different areas of GBM and determine if methylation status varied depending on the fixation technique (paraffin-embedding versus fresh frozen) used to store tissue. Using intraoperative navigation, biopsies were obtained from three distinct regions: the enhancing outer area, the non-enhancing inner core, and an area immediately outside the enhancing region. Only patients with GBM were included for evaluation and analysis. Samples taken from each area were divided with half stored by flash freezing and the other half stored using paraffin fixation. Methylation Specific-PCR (MS-PCR) was used for analysis of MGMT promoter methylation. Thirteen patients were included. Ten were male with a median age of 62 years. In each patient, samples were taken from the enhancing rim and the necrotic centre. However, it was not considered safe or feasible to obtain samples from the area immediately adjacent to the enhancing tumor rim in one case. All patients were homogeneous for methylation status throughout their tumor and tissue taken adjacent to it when frozen tissue was used. However, four patients had discrepancies in the MGMT promoter status between the frozen and paraffin-embedded blocks and one patient was not homogeneous within the tumor when paraffin-embedded tissue was used. MGMT promoter methylation status was homogeneous in all GBM tumors. Our observation that methylation status varied depending if the DNA was extracted from paraffin-embedded versus frozen tissue is concerning. Although the reason for this is unclear, we postulate that the timing from resection to fixation or the process of fixation itself may potentially alter methylation status in paraffin-embedded tumors.

Dissolved organic carbon and nitrogen release from boreal Holocene permafrost and seasonally frozen soils of Alaska

NASA Astrophysics Data System (ADS)

Wickland, Kimberly P.; Waldrop, Mark P.; Aiken, George R.; Koch, Joshua C.; Torre Jorgenson, M.; Striegl, Robert G.

2018-06-01

Permafrost (perennially frozen) soils store vast amounts of organic carbon (C) and nitrogen (N) that are vulnerable to mobilization as dissolved organic carbon (DOC) and dissolved organic and inorganic nitrogen (DON, DIN) upon thaw. Such releases will affect the biogeochemistry of permafrost regions, yet little is known about the chemical composition and source variability of active-layer (seasonally frozen) and permafrost soil DOC, DON and DIN. We quantified DOC, total dissolved N (TDN), DON, and DIN leachate yields from deep active-layer and near-surface boreal Holocene permafrost soils in interior Alaska varying in soil C and N content and radiocarbon age to determine potential release upon thaw. Soil cores were collected at three sites distributed across the Alaska boreal region in late winter, cut in 15 cm thick sections, and deep active-layer and shallow permafrost sections were thawed and leached. Leachates were analyzed for DOC, TDN, nitrate (NO3 ‑), and ammonium (NH4 +) concentrations, dissolved organic matter optical properties, and DOC biodegradability. Soils were analyzed for C, N, and radiocarbon (14C) content. Soil DOC, TDN, DON, and DIN yields increased linearly with soil C and N content, and decreased with increasing radiocarbon age. These relationships were significantly different for active-layer and permafrost soils such that for a given soil C or N content, or radiocarbon age, permafrost soils released more DOC and TDN (mostly as DON) per gram soil than active-layer soils. Permafrost soil DOC biodegradability was significantly correlated with soil Δ14C and DOM optical properties. Our results demonstrate that near-surface Holocene permafrost soils preserve greater relative potential DOC and TDN yields than overlying seasonally frozen soils that are exposed to annual leaching and decomposition. While many factors control the fate of DOC and TDN, the greater relative yields from newly thawed Holocene permafrost soils will have the largest potential impact in areas dominated by organic-rich soils.

CSU-CHILL Polarimetric Radar Measurements from a Severe Hail Storm in Eastern Colorado.

NASA Astrophysics Data System (ADS)

Hubbert, J.; Bringi, V. N.; Carey, L. D.; Bolen, S.

1998-08-01

Polarimetric radar measurements made by the recently upgraded CSU-CHILL radar system in a severe hailstorm are analyzed permitting for the first time the combined use of Zh, ZDR, linear depolarization ratio (LDR), KDP, and h to infer hydrometeor types. A chase van equipped for manual collection of hail, and instrumented with a rain gauge, intercepted the storm core for 50 min. The period of golfball-sized hail is easily distinguished by high LDR (greater than or equal to 18 dB), negative ZDR (less than or equal to 0.5 dB), and low h (less than or equal to 0.93) values near the surface. Rainfall accumulation over the entire event (about 40 mm) estimated using KDP is in excellent agreement with the rain gauge measurement. Limited dual-Doppler synthesis using the CSU-CHILL and Denver WSR-88D radars permit estimates of the horizontal convergence at altitudes less than 3 km above ground level (AGL) at 1747 and 1812 mountain daylight time (MDT). Locations of peak horizontal convergence at these times are centered on well-defined positive ZDR columns. Vertical sections of multiparameter radar data at 1812 MDT are interpreted in terms of hydrometeor type. In particular, an enhanced LDR `cap' area on top of the the positive ZDR column is interpreted as a region of mixed phase with large drops mixed with partially frozen and frozen hydrometeors. A positive KDP column on the the western fringe of the main updraft is inferred to be the result of drops (1-2 mm) shed by wet hailstones. Swaths of large hail at the surface (inferred from LDR signatures) and positive ZDR at 3.5 km AGL suggest that potential frozen drop embryos are favorably located for growth into large hailstones. Thin section analysis of a sample of the large hailstones shows that 30%-40% have frozen drop embryos.

Understanding the Star Formation Process in the Filamentary Dark Cloud GF 9: Near-Infrared Observations

NASA Technical Reports Server (NTRS)

Ciardi, David R.; Woodward, Charles E.; Clemens, Dan P.; Harker, David E.; Rudy, Richard J.

1998-01-01

We have performed a near-infrared JHK survey of a dense core and a diffuse filament region within the filamentary dark cloud GF 9 (LDN 1082). The core region is associated with the IRAS point source PSC 20503+6006 and is suspected of being a site of star formation. The diffuse filament region has no associated IRAS point sources and is likely quiescent. We find that neither the core nor the filament region appears to contain a Class I or Class II young stellar object. As traced by the dust extinction, the core and filament regions contain 26 and 22 solar mass, respectively, with an average H2 volume density for both regions of approximately 2500/cu cm. The core region contains a centrally condensed extinction maximum with a peak extinction of A(sub v) greater than or approximately equal to 10 mag that appears to be associated with the IRAS point source. The average H2 volume density of the extinction core is approximately 8000/cu cm. The dust within the filament, however, shows no sign of a central condensation and is consistent with a uniform-density cylindrical distribution.

The 1500m South Pole Ice Core: Recovering a 40 Ka Environmental Record

NASA Technical Reports Server (NTRS)

Casey, Kimberly Ann; Neumann, Thomas Allen; Fudge, T. J.; Neumann, T. A.; Steig, E. J.; Cavitte, M. G. P.; Blankenship, D. D.

2014-01-01

Supported by the US National Science Foundation, a new 1500 m, approximately 40 ka old ice core will be recovered from South Pole during the 2014/15 and 2015/16 austral summer seasons using the new US Intermediate Depth Drill. The combination of low temperatures, relatively high accumulation rates and low impurity concentrations at South Pole will yield detailed records of ice chemistry and trace atmospheric gases. The South Pole ice core will provide a climate history record of a unique area of the East Antarctic plateau that is partly influenced by weather systems that cross the West Antarctic ice sheet. The ice at South Pole flows at approximately 10m a(exp-1) and the South Pole ice-core site is a significant distance from an ice divide. Therefore, ice recovered at depth originated progressively farther upstream of the coring site. New ground-penetrating radar collected over the drill site location shows no anthropogenic influence over the past approximately 50 years or upper 15 m. Depth-age scale modeling results show consistent and plausible annual-layer thicknesses and accumulation rate histories, indicating that no significant stratigraphic disturbances exist in the upper 1500m near the ice-core drill site.

Characterizing probe performance in the aberration corrected STEM.

PubMed

Batson, P E

2006-01-01

Sub-Angstrom imaging using the 120 kV IBM STEM is now routine if the probe optics is carefully controlled and fully characterized. However, multislice simulation using at least a frozen phonon approximation is required to understand the Annular Dark Field image contrast. Analysis of silicon dumbbell structures in the [110] and [211] projections illustrate this finding. Using fast image acquisition, atomic movement appears ubiquitous under the electron beam, and may be useful to illuminate atomic level processes.

Control of π-Electron Rotations in Chiral Aromatic Molecules Using Intense Laser Pulses

NASA Astrophysics Data System (ADS)

Kanno, Manabu; Kono, Hirohiko; Fujimura, Yuichi

Our recent theoretical studies on laser-induced π-electron rotations in chiral aromatic molecules are reviewed. π electrons of a chiral aromatic molecule can be rotated along its aromatic ring by a nonhelical, linearly polarized laser pulse. An ansa aromatic molecule with a six-membered ring, 2,5-dichloro[n](3,6) pyrazinophane, which belongs to a planar-chiral molecule group, and its simplified molecule 2,5-dichloropyrazine are taken as model molecules. Electron wavepacket simulations in the frozen-molecular-vibration approximation show that the initial direction of π-electron rotation depends on the polarization direction of a linearly polarized laser pulse applied. Consecutive unidirectional rotation can be achieved by applying a sequence of linearly polarized pump and dump pulses to prevent reverse rotation. Optimal control simulations of π-electron rotation show that another controlling factor for unidirectional rotation is the relative optical phase between the different frequency components of an incident pulse in addition to photon polarization direction. Effects of nonadiabatic coupling between π-electron rotation and molecular vibrations are also presented, where the constraints of the frozen approximation are removed. The angular momentum gradually decays mainly owing to nonadiabatic coupling, while the vibrational amplitudes greatly depend on their rotation direction. This suggests that the direction of π-electron rotation on an attosecond timescale can be identified by detecting femtosecond molecular vibrations.

Frozen Gaussian approximation for 3D seismic tomography

NASA Astrophysics Data System (ADS)

Chai, Lihui; Tong, Ping; Yang, Xu

2018-05-01

Three-dimensional (3D) wave-equation-based seismic tomography is computationally challenging in large scales and high-frequency regime. In this paper, we apply the frozen Gaussian approximation (FGA) method to compute 3D sensitivity kernels and seismic tomography of high-frequency. Rather than standard ray theory used in seismic inversion (e.g. Kirchhoff migration and Gaussian beam migration), FGA is used to compute the 3D high-frequency sensitivity kernels for travel-time or full waveform inversions. Specifically, we reformulate the equations of the forward and adjoint wavefields for the purpose of convenience to apply FGA, and with this reformulation, one can efficiently compute the Green’s functions whose convolutions with source time function produce wavefields needed for the construction of 3D kernels. Moreover, a fast summation method is proposed based on local fast Fourier transform which greatly improves the speed of reconstruction as the last step of FGA algorithm. We apply FGA to both the travel-time adjoint tomography and full waveform inversion (FWI) on synthetic crosswell seismic data with dominant frequencies as high as those of real crosswell data, and confirm again that FWI requires a more sophisticated initial velocity model for the convergence than travel-time adjoint tomography. We also numerically test the accuracy of applying FGA to local earthquake tomography. This study paves the way to directly apply wave-equation-based seismic tomography methods into real data around their dominant frequencies.

DDTs and HCHs in sediment cores from the Tibetan Plateau.

PubMed

Cheng, Hairong; Lin, Tian; Zhang, Gan; Liu, Guoqing; Zhang, Weiling; Qi, Shihua; Jones, Kevin C; Zhang, Xuewen

2014-01-01

Sediment cores were collected from five critical regions in the Tibetan Plateau and were analysed for OCPs with the objective of examining the time trends and recycling of DDTs and HCHs in the cryogenic area. A concurrent increase of the DDT and HCH concentrations from the late 1980s in Lake Yamzho Yumco, Nam Co and Star Sea were observed. The increasing levels of DDE/DDTs (>0.4) suggested that DDT in the upper layers of the sediment cores may be recycled/"weathered" DDT. Regarding the acceleration of glacier retreat from the 1980s due to global warming, it is suggested that OCPs formerly trapped either in the snow/glacier or in the frozen soil land recently reclaimed in the processes of glacier retreat may have been flushed into the sedimentary basins. These findings demonstrate the potential impact of global warming on the recycling of POPs in the plateau cryosphere and indicate that the pristine Tibetan Plateau may serve as one of the key probes to the global trend of POPs. Copyright © 2013 Elsevier Ltd. All rights reserved.

Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection.

PubMed

Yasmin, Nusrat; Saleem, Mahjabeen; Naz, Mamoona; Gul, Roquyya; Rehman, Hafiz Muzzammel

2017-01-01

A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli . Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E. coli BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A. fumigatus ; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against A. fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.

Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection

PubMed Central

Yasmin, Nusrat; Naz, Mamoona; Gul, Roquyya; Rehman, Hafiz Muzzammel

2017-01-01

A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E. coli BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A. fumigatus; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against A. fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications. PMID:28875151

Desorption induced by solar wind electrons analogs in methanol ice

NASA Astrophysics Data System (ADS)

Bergantini, A. S.; Pilling, Sergio; Andrade, Diana; Boechat-Roberty, Heloisa Maria; Rocco, Maria Luiza M.

2012-07-01

Methanol (CH _{3}OH) has been detected in several environments in space, such as comets, asteroids, grains of interstellar dust and protostars forming regions such as W33A and RAFGL 7009. CH _{3}OH is the most abundant molecule (after H _{2}O) found in solid state in these objects. The action of ionizing agents in such environments induces changes in methanol ice which lead to the formation ionic species, reactive radicals and new compounds. In this experiment, frozen methanol (142 K) was irradiated with an electron beam (energies from 650 to 1500 eV) inside an ultra-high vacuum chamber (˜ 1×10^{-10} mbar), at the Surface Chemistry Laboratory of Federal University of Rio de Janeiro (LaQuiS/ UFRJ). The beam simulates the action of electrons from solar wind in frozen surfaces like as comets, asteroids and moons. Results show the desorption of several new ionized species such as (CH _{3}OH)H ^{+}, H _{2}COH ^{+}, C _{2}H _{3} ^{+}, HCO ^{+}, CO ^{+}, O ^{+}, C ^{+}, H ^{+}. The individual desorbed ion rate was calculated. The determined half-life of frozen methanol in Earth orbit due to the electron bombardment was about 4.2 yr. The ionic desorption rate is an important parameter in surface chemistry, since these parameters are often approximated in chemical evolution models of astrophysical environments, due to the lack of laboratory data.

Meat Composition and Quality Assessment of King Scallops (Pecten maximus) and Frozen Atlantic Sea Scallops (Placopecten magellanicus) on a Retail Level

PubMed Central

Manthey-Karl, Monika; Lehmann, Ines; Ostermeyer, Ute; Rehbein, Hartmut; Schröder, Ute

2015-01-01

An enlarged range of scallop products on the market allows the consumer to buy lower priced alternatives, which often raises the question of quality and control. Frozen meat of king scallops (Pecten maximus) and Atlantic sea scallops (Placopecten magellanicus) were purchased on the German market and compared with fresh shell-on king scallops of various origin. The approximate composition, inclusive citric acid and phosphates, minerals, free amino acids (FAA) and fatty acid profiles were examined in the muscle to identify changes as a result of processing. The FAA glycine and taurine as well the fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) were the most abundant, but were reduced in processed samples. Di- and triphosphate contents were not detectable (<0.01 g·kg−1) in untreated meats. Most frozen scallop products contained added citrates and polyphosphates and had distinctly higher water contents (up to 89%) and an increased moisture to protein ratio (M/P) (up to 9) compared with the fresh king scallops (78%, M/P < 5). Labelling of species, verified by PCR-based DNA analysis, and ingredients were not correct in each case. Overall results indicated no relevant differences in mineral content, except high sodium contents, resulting from additives. Labelling does not readily allow the consumer to recognize the extent of processing effects. PMID:28231221

Matched case-control phase 2 study to evaluate the use of a frozen sock to prevent docetaxel-induced onycholysis and cutaneous toxicity of the foot.

PubMed

Scotté, Florian; Banu, Eugeniu; Medioni, Jacques; Levy, Eric; Ebenezer, Christelle; Marsan, Sandrine; Banu, Adela; Tourani, Jean Marc; Andrieu, Jean-Marie; Oudard, Stéphane

2008-04-01

Onycholysis occurs in approximately 30% of patients treated with docetaxel. The efficacy and safety of an Elasto-Gel frozen sock (FS) was investigated for the prevention of docetaxel-induced nail and skin toxicity of the feet. Patients receiving docetaxel at a dose of 70 to 100 mg/m(2) every 3 weeks were eligible for this matched case-control study. Each patient wore an FS for 90 minutes on the right foot. The unprotected left foot acted as control. Nail and skin toxicities were assessed using National Cancer Institute Common Toxicity Criteria (version 3) and compared using a 2-sample Wilcoxon matched-pairs rank test adjusted for tied values. Fifty consecutive patients were included between April 2005 and January 2007. Nail toxicity was significantly lower in the FS-protected foot compared with the control foot (grade 0: 100% versus 79%; and grade 1 and 2: 0% versus 21%, respectively) (P= .002). Skin toxicity was grade 0: 98% versus 94%; and grade 1 and 2: 2% versus 6% in the FS-protected and the control feet, respectively. The median times until toxicity occurrence were not found to differ significantly between the groups. One patient experienced discomfort because of cold intolerance. Cold therapy using FS significantly reduced the incidence of docetaxel-induced foot nail toxicity, as previously demonstrated using frozen gloves for the hands.

Does freezing and dynamic flexing of frozen branches impact the cavitation resistance of Malus domestica and the Populus clone Walker?

PubMed

Christensen-Dalsgaard, Karen K; Tyree, Melvin T

2013-11-01

Frost damage to the xylem conduits of trees is a phenomenon of eco-physiological importance. It is often documented in terms of the percentage loss of conductivity (PLC), an indicator of air filling of the conduits. However, trees that refill their conduits in spring could be impacted more by damage to the conduits that reduce cavitation resistance, making them more susceptible to future drought events. We investigated whether ice formation, dynamic flexing of frozen branches or freeze-thaw events could reduce the cavitation resistance (cause "frost fatigue") in first-year shoots of apple (Malus domestica) and clonal hybrid cottonwood (Walker). Frost fatigue was measured in terms of P50 (the negative xylem pressure required to cause a 50 % loss of conductivity). All treatment groups showed significant frost fatigue, with the exception of the pre-flushed, constantly frozen poplar branches. The P50 following freeze treatments was approximately 50 % of the pre-freeze values. The effect tended to be greater in freeze-thawed branches. Dynamic bending of the branches had no effect on either PLC or P50. In three out of four cases, there was a significant correlation between P50 and PLC. Frost fatigue occurred in both apple and poplar, two unrelated species with different drought and frost tolerances, suggesting that it may be a widespread phenomenon that could impact the ecophysiology of temperate forests.

Impact craters as biospheric microenvironments, Lawn Hill Structure, Northern Australia.

PubMed

Lindsay, John; Brasier, Martin

2006-04-01

Impact craters on Mars act as traps for eolian sediment and in the past may have provided suitable microenvironments that could have supported and preserved a stressed biosphere. If this is so, terrestrial impact structures such as the 18-km-diameter Lawn Hill Structure, in northern Australia, may prove useful as martian analogs. We sampled outcrop and drill core from the carbonate fill of the Lawn Hill Structure and recorded its gamma-log signature. Facies data along with whole rock geochemistry and stable isotope signatures show that the crater fill is an outlier of the Georgina Basin and was formed by impact at, or shortly before, approximately 509-506 million years ago. Subsequently, it was rapidly engulfed by the Middle Cambrian marine transgression, which filled it with shallow marine carbonates and evaporites. The crater formed a protected but restricted microenvironment in which sediments four times the thickness of the nearby basinal succession accumulated. Similar structures, common on the martian surface, may well have acted as biospheric refuges as the planet's water resources declined. Low-pH aqueous environments on Earth similar to those on Mars, while extreme, support diverse ecologies. The architecture of the eolian crater fill would have been defined by long-term ground water cycles resulting from intermittent precipitation in an extremely arid climate. Nutrient recycling, critical to a closed lacustrine sub-ice biosphere, could be provided by eolian transport onto the frozen water surface.

Million-body star cluster simulations: comparisons between Monte Carlo and direct N-body

NASA Astrophysics Data System (ADS)

Rodriguez, Carl L.; Morscher, Meagan; Wang, Long; Chatterjee, Sourav; Rasio, Frederic A.; Spurzem, Rainer

2016-12-01

We present the first detailed comparison between million-body globular cluster simulations computed with a Hénon-type Monte Carlo code, CMC, and a direct N-body code, NBODY6++GPU. Both simulations start from an identical cluster model with 106 particles, and include all of the relevant physics needed to treat the system in a highly realistic way. With the two codes `frozen' (no fine-tuning of any free parameters or internal algorithms of the codes) we find good agreement in the overall evolution of the two models. Furthermore, we find that in both models, large numbers of stellar-mass black holes (>1000) are retained for 12 Gyr. Thus, the very accurate direct N-body approach confirms recent predictions that black holes can be retained in present-day, old globular clusters. We find only minor disagreements between the two models and attribute these to the small-N dynamics driving the evolution of the cluster core for which the Monte Carlo assumptions are less ideal. Based on the overwhelming general agreement between the two models computed using these vastly different techniques, we conclude that our Monte Carlo approach, which is more approximate, but dramatically faster compared to the direct N-body, is capable of producing an accurate description of the long-term evolution of massive globular clusters even when the clusters contain large populations of stellar-mass black holes.

Carbon Solubility in Silicon-Iron-Bearing Metals during Core Formation on Mercury

NASA Technical Reports Server (NTRS)

Vander Kaaden, Kathleen E.; McCubbin, Francis M.; Ross, D. Kent; Rapp, Jennifer F.; Danielson, Lisa R.; Keller, Lindsay P.; Righter, Kevin

2016-01-01

Recent results obtained from the MErcury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) spacecraft showed the surface of Mercury has high S abundances (approximately 4 wt%) and low Iron(II) Oxide abundances (less than 2 wt%). Based on these extreme values, the oxygen fugacity of Mercury's surface materials was estimated to be approximately 3 to 7 log(sub 10) units below the IW buffer (Delta IW-3 to Delta IW-7). This highly reducing nature of the planet has resulted in a large core and relatively thin mantle, extending to only approximately 420 km depth (corresponding to a core-mantle boundary pressure of approximately 4-7 GPa) within the planet. Furthermore, MESSENGER results have suggested the presence of carbon on the surface of the planet. Previous experimental results from have also suggested the possibility of a primary floatation crust on Mercury composed of graphite, produced after a global magma ocean event. With these exotic conditions of this compositional end-member planet, it begs the question, what is the core composition of Mercury? Although no definitive conclusion has been reached, previous studies have made advances towards answering this question. Riner et al. and Chen et al. looked at iron sulfide systems and implemented various crystallization and layered core scenarios to try and determine the composition and structure of Mercury's core. Malavergne et al. examined core crystallization scenarios in the presence of sulfur and silicon. Hauck et al. used the most recent geophysical constraints from the MESSENGER spacecraft to model the internal structure of Mercury, including the core, in a iron-sulfur-silicon system. More recently, Chabot et al. conducted a series of metal-silicate partitioning experiments in a iron-sulfur-silicon system. These results showed the core of Mercury has the potential to contain more than 15 wt% silicon. However, with the newest results from MESSENGER's low altitude campaign, carbon is another potential light element that could be incorporated into Mercury's core. The goal of this study is to determine the carbon concentration at graphite saturation in various silicon-iron bearing metals relevant to possible mercurian core compositions. Future experiments will include the addition of sulfur into these metals.

Immunogold Staining of Ultrathin Thawed Cryosections for Transmission Electron Microscopy (TEM).

PubMed

Skepper, Jeremy N; Powell, Janet M

2008-06-01

INTRODUCTIONA pre-embedding method of immunochemical staining is used if antigens are damaged by resin embedding, or if the best preservation of membranes is required. Applying immunogold reagents to sections of lightly fixed tissue, free of embedding medium, can be a very sensitive method of immunochemical staining. Cells or tissues are fixed as strongly as possible and then treated with a cryoprotectant, which is usually a mixture of sucrose and polyvinylpyrrolidone (PVP). They are frozen onto pins in liquid nitrogen and sectioned at approximately -100°C. The frozen sections are thaw-mounted on to Formvar/nickel film grids and the cryoprotectant is removed by floating the grids on drops of phosphate-buffered saline (PBS). The immunogold staining is performed on the unembedded sections, which are subsequently contrast counterstained and infiltrated with a mixture of methylcellulose and uranyl acetate. In this protocol, samples are sectioned at low temperature, thaw-mounted onto film grids, immunochemically stained, contrast counterstained, and embedded/encapsulated in situ on the grid before viewing by transmission electron microscopy (TEM).

Dynamic behavior of an unsteady trubulent boundary layer

NASA Technical Reports Server (NTRS)

Parikh, P. G.; Reynolds, W. C.; Jayaramen, R.; Carr, L. W.

1981-01-01

Experiments on an unsteady turbulent boundary layer are reported in which the upstream portion of the flow is steady (in the mean) and in the downstream region, the boundary layer sees a linearly decreasing free stream velocity. This velocity gradient oscillates in time, at frequencies ranging from zero to approximately the bursting frequency. For the small amplitude, the mean velocity and mean turbulence intensity profiles are unaffected by the oscillations. The amplitude of the periodic velocity component, although as much as 70% greater than that in the free stream for very low frequencies, becomes equal to that in the free stream at higher frequencies. At high frequencies, both the boundary layer thickness and the Reynolds stress distribution across the boundary layer become frozen. The behavior at higher amplitude is quite similar. At sufficiently high frequencies, the boundary layer thickness remains frozen at the mean value over the oscillation cycle, even though flow reverses near the wall during a part of the cycle.

The Last Interglacial sea level change: new evidence from the Abrolhos islands, West Australia

NASA Astrophysics Data System (ADS)

Eisenhauer, A.; Zhu, Z. R.; Collins, L. B.; Wyrwoll, K. H.; Eichstätter, R.

U-series ages measured by thermal ionisation mass spectrometry (TIMS) are reported for a Last Interglacial (LI) fossil coral core from the Turtle Bay, Houtman Abrolhos islands, western Australia. The core is 33.4m long the top of which is approximately 5ma.p.s.l. (above present sea level). From the 232Th concentrations and the reliability of the U-series ages, two sections in the core can be distinguished. Calculated U/Th ages in core sectionI (3.3ma.p.s.l to 11mb.p.s.l) vary between 124+/-1.7kaBP (3.3ma.p.s.l.) and 132.5+/-1.8ka (4mb.p.s.l., i.e. below present sea level), and those of sectionII (11-23mb.p.s.l.) between 140+/-3 and 214+/-5kaBP, respectively. The ages of core sectionI are in almost perfect chronological order, whereas for sectionII no clear age-depth relationship of the samples can be recognised. Further assessments based on the ∂234U(T) criteria reveal that none of the samples of core sectionII give reliable ages, whereas for core sectionI several samples can be considered to be moderately reliable within 2ka. The data of the Turtle Bay core complement and extend our previous work from the Houtman Abrolhos showing that the sea level reached a height of approximately 4mb.p.s.l at approximately 134kaBP and a sea level highstand of at least 3.3ma.p.s.l. at approximately 124kaBP. Sea level dropped below its present position at approximately 116kaBP. Although the new data are in general accord with the Milankovitch theory of climate change, a detailed comparison reveals considerable differences between the Holocenand LI sea level rise as monitored relative to the Houtman Abrolhos islands. These observation apparently add further evidence to the growing set of data that the LI sea level rise started earlier than recognised by SPECMAP chronology. A reconciliation of these contradictionary observations following the line of arguments presented by Crowley (1994) are discussed with respect to the Milankovitch theory.

Analysis and evaluation of WRF microphysical schemes for deep moist convection over south-eastern South America (SESA) using microwave satellite observations and radiative transfer simulations

NASA Astrophysics Data System (ADS)

Sol Galligani, Victoria; Wang, Die; Alvarez Imaz, Milagros; Salio, Paola; Prigent, Catherine

2017-10-01

In the present study, three meteorological events of extreme deep moist convection, characteristic of south-eastern South America, are considered to conduct a systematic evaluation of the microphysical parameterizations available in the Weather Research and Forecasting (WRF) model by undertaking a direct comparison between satellite-based simulated and observed microwave radiances. A research radiative transfer model, the Atmospheric Radiative Transfer Simulator (ARTS), is coupled with the WRF model under three different microphysical parameterizations (WSM6, WDM6 and Thompson schemes). Microwave radiometry has shown a promising ability in the characterization of frozen hydrometeors. At high microwave frequencies, however, frozen hydrometeors significantly scatter radiation, and the relationship between radiation and hydrometeor populations becomes very complex. The main difficulty in microwave remote sensing of frozen hydrometeor characterization is correctly characterizing this scattering signal due to the complex and variable nature of the size, composition and shape of frozen hydrometeors. The present study further aims at improving the understanding of frozen hydrometeor optical properties characteristic of deep moist convection events in south-eastern South America. In the present study, bulk optical properties are computed by integrating the single-scattering properties of the Liu(2008) discrete dipole approximation (DDA) single-scattering database across the particle size distributions parameterized by the different WRF schemes in a consistent manner, introducing the equal mass approach. The equal mass approach consists of describing the optical properties of the WRF snow and graupel hydrometeors with the optical properties of habits in the DDA database whose dimensions might be different (Dmax') but whose mass is conserved. The performance of the radiative transfer simulations is evaluated by comparing the simulations with the available coincident microwave observations up to 190 GHz (with observations from Tropical Rainfall Measuring Mission's (TRMM) Microwave Imager (TMI), Microwave Humidity Sounder (MHS) and Special Sensor Microwave Imager/Sounder (SSMI/S)) using the χ2 test. Good agreement is obtained with all observations provided special care is taken to represent the scattering properties of the snow and graupel species.

New radiocarbon ages from cirques in Colorado Front Range

DOE Office of Scientific and Technical Information (OSTI.GOV)

Davis, P.T.; Birkeland, P.W.; Caine, N.

The authors recovered sediment cores 3.1 m long from Blue Lake ([approximately]37m water depth, [approximately]3,445m a.s.l., 40[degree]5 minutes 20 seconds N, 105[degree]37 minutes 08 seconds W) and 2.7m long from Lake Dorothy ([approximately]35m water depth, [approximately]3,675m a.s.l., 40[degree]00 minutes 46 seconds N, 105[degree]41 minutes 11 seconds W). A light-weight percussion coring system suspended from perlon ropes was used because of sediment thicknesses, water depths, and ski-backpacking requirements. Lake ice provided a stable coring platform. One purpose of the project is provision of a high-resolution record of environmental change in the subalpine/alpine ecotone during the Holocene, under the auspices of themore » Niwot Ridge Long-Term Ecological Research program. The sediment cores also provide minimum-limiting radiocarbon ages for deglaciation of cirques and the deposits that impound their tarns. Here the authors report on this second purpose. The Blue Lake core bottomed in sandy, gray, inorganic sediment, presumably glacial diamict. A bulk sample from 2.8--2.9m depth yielded a conventional radiocarbon age of 12,275[+-]345 yrs BP. Thus, ice retreated from the site by 12 ka. Since 12 ka both glacial and rock-glacial sediments have been deposited upvalley; some of these events may be recognized in the core. In contrast, the Lake Dorothy core did not penetrate gray inorganic diamict and is entirely organic-rich. A bulk sample from 2.65--2.7m depth yielded a conventional radiocarbon age of 10,910 [+-] 320 yrs BP. Thus, the moraines impounding the lake are 2--3 times older than suggested by a combination of relative-age methods and one radiocarbon age from surface sediments.« less

Possible Geological Records of the Symbiotic Binary R Aquarii's Historical Outbursts

NASA Astrophysics Data System (ADS)

Tanabe, Kenji

2015-08-01

R Aquarii, known as one of the most enigmatic variable stars, seems to experience several outbursts as suggested by its surrounding nebulosity. Fortunately,in Korean ancient official books two outbursts in A.D.1073 and 1074 are recorded precisely (both its position and brightness). These two events possibly coincide with the two prominent spikes of nitrate ion frozen in the ice core extracted in 2001 from the top of Dome Fuji(3810 mheight) at the Japanese Antarctic station.We shall discuss whether such a coincidence is plausible or not from the point of viw of time resolution and age determination.

Method For Removing Volatile Components From A Gel-Cast Ceramic Article

DOEpatents

Klug, Frederic Joseph; DeCarr, Sylvia Marie

2004-09-07

A method of removing substantially all of the volatile component in a green, volatile-containing ceramic article is disclosed. The method comprises freezing the ceramic article; and then subjecting the frozen article to a vacuum for a sufficient time to freeze-dry the article. Frequently, the article is heated while being freeze-dried. Use of this method efficiently reduces the propensity for any warpage of the article. The article is often formed from a ceramic slurry in a gel-casting process. A method for fabricating a ceramic core used in investment casting is also described.

Method for removing volatile components from a ceramic article, and related processes

DOEpatents

Klug, Frederic Joseph; DeCarr, Sylvia Marie

2002-01-01

A method of removing substantially all of the volatile component in a green, volatile-containing ceramic article is disclosed. The method comprises freezing the ceramic article; and then subjecting the frozen article to a vacuum for a sufficient time to freeze-dry the article. Frequently, the article is heated while being freeze-dried. Use of this method efficiently reduces the propensity for any warpage of the article. The article is often formed from a ceramic slurry in a gel-casting process. A method for fabricating a ceramic core used in investment casting is also described.

Determination of magnetic helicity in the solar wind and implications for cosmic ray propagation

NASA Technical Reports Server (NTRS)

Matthaeus, W. M.; Goldstein, M. L.

1981-01-01

Magnetic helicity (Hm) is the mean value of the correlation between a turbulent magnetic field and the magnetic vector potential. A technique is described for determining Hm and its 'reduced' spectrum from the two point magnetic correlation matrix. The application of the derived formalism to solar wind magnetic fluctuations is discussed, taking into account cases for which only single point measurements are available. The application procedure employs the usual 'frozen in approximation' approach. The considered method is applied to an analysis of several periods of Voyager 2 interplanetary magnetometer data near 2.8 AU. During these periods the correlation length, or energy containing length, was found to be approximately 3 x 10 to the 11th cm

Demagnetization using a determined estimated magnetic state

DOEpatents

Denis, Ronald J; Makowski, Nathanael J

2015-01-13

A method for demagnetizing comprising positioning a core within the electromagnetic field generated by a first winding until the generated first electrical current is not substantially increasing, thereby determining a saturation current. A second voltage, having the opposite polarity, is then applied across the first winding until the generated second electrical current is approximately equal to the magnitude of the determined saturation current. The maximum magnetic flux within the core is then determined using the voltage across said first winding and the second current. A third voltage, having the opposite polarity, is then applied across the first winding until the core has a magnetic flux equal to approximately half of the determined maximum magnetic flux within the core.

21 CFR 160.110 - Frozen eggs.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen eggs. 160.110 Section 160.110 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.110 Frozen eggs. (a) Frozen eggs, frozen whole eggs, frozen mixed eggs is the food prepared by freezing...

21 CFR 160.110 - Frozen eggs.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen eggs. 160.110 Section 160.110 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.110 Frozen eggs. (a) Frozen eggs, frozen whole eggs, frozen mixed eggs is the food prepared by freezing...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sherman, Andrew J

A heterogeneous body having ceramic rich cermet regions in a more ductile metal matrix. The heterogeneous bodies are formed by thermal spray operations on metal substrates. The thermal spray operations apply heat to a cermet powder and project it onto a solid substrate. The cermet powder is composed of complex composite particles in which a complex ceramic-metallic core particle is coated with a matrix precursor. The cermet regions are generally comprised of complex ceramic-metallic composites that correspond approximately to the core particles. The cermet regions are approximately lenticular shaped with an average width that is at least approximately twice themore » average thickness. The cermet regions are imbedded within the matrix phase and generally isolated from one another. They have obverse and reverse surfaces. The matrix phase is formed from the matrix precursor coating on the core particles. The amount of heat applied during the formation of the heterogeneous body is controlled so that the core particles soften but do not become so fluid that they disperse throughout the matrix phase. The force of the impact on the surface of the substrate tends to flatten them. The flattened cermet regions tend to be approximately aligned with one another in the body.« less

Semiclassical neutral atom as a reference system in density functional theory.

PubMed

Constantin, Lucian A; Fabiano, E; Laricchia, S; Della Sala, F

2011-05-06

We use the asymptotic expansions of the semiclassical neutral atom as a reference system in density functional theory to construct accurate generalized gradient approximations (GGAs) for the exchange-correlation and kinetic energies without any empiricism. These asymptotic functionals are among the most accurate GGAs for molecular systems, perform well for solid state, and overcome current GGA state of the art in frozen density embedding calculations. Our results also provide evidence for the conjointness conjecture between exchange and kinetic energies of atomic systems.

Carbon-silicon core-shell nanowires as high capacity electrode for lithium ion batteries.

PubMed

Cui, Li-Feng; Yang, Yuan; Hsu, Ching-Mei; Cui, Yi

2009-09-01

We introduce a novel design of carbon-silicon core-shell nanowires for high power and long life lithium battery electrodes. Amorphous silicon was coated onto carbon nanofibers to form a core-shell structure and the resulted core-shell nanowires showed great performance as anode material. Since carbon has a much smaller capacity compared to silicon, the carbon core experiences less structural stress or damage during lithium cycling and can function as a mechanical support and an efficient electron conducting pathway. These nanowires have a high charge storage capacity of approximately 2000 mAh/g and good cycling life. They also have a high Coulmbic efficiency of 90% for the first cycle and 98-99.6% for the following cycles. A full cell composed of LiCoO(2) cathode and carbon-silicon core-shell nanowire anode is also demonstrated. Significantly, using these core-shell nanowires we have obtained high mass loading and an area capacity of approximately 4 mAh/cm(2), which is comparable to commercial battery values.

The Hydromagnetic Interior of a Solar Quiescent Prominence. I. Coupling between Force Balance and Steady Energy Transport

NASA Astrophysics Data System (ADS)

Low, B. C.; Berger, T.; Casini, R.; Liu, W.

2012-08-01

This series of papers investigates the dynamic interiors of quiescent prominences revealed by recent Hinode and SDO/AIA high-resolution observations. This first paper is a study of the static equilibrium of the Kippenhahn-Schlüter diffuse plasma slab, suspended vertically in a bowed magnetic field, under the frozen-in condition and subject to a theoretical thermal balance among an optically thin radiation, heating, and field-aligned thermal conduction. The everywhere-analytical solutions to this nonlinear problem are an extremely restricted subset of the physically admissible states of the system. For most values of the total mass frozen into a given bowed field, force balance and steady energy transport cannot both be met without a finite fraction of the total mass having collapsed into a cold sheet of zero thickness, within which the frozen-in condition must break down. An exact, resistive hydromagnetic extension of the Kippenhahn-Schlüter slab is also presented, resolving the mass-sheet singularity into a finite-thickness layer of steadily falling dense fluid. Our hydromagnetic result suggests that the narrow, vertical prominence Hα threads may be falling across magnetic fields, with optically thick cores much denser and ionized to much lower degrees than conventionally considered. This implication is discussed in relation to (1) the recent SDO/AIA observations of quiescent prominences that are massive and yet draining mass everywhere in their interiors, (2) the canonical range of 5-60 G determined from spectral polarimetric observations of prominence magnetic fields over the years, and (3) the need for a more realistic multi-fluid treatment.

Terminologie alimentaire (Food Terminology).

ERIC Educational Resources Information Center

Pelletier, Jean-Francois

1980-01-01

Translations and descriptions are given in French for a number of English food terms: convenience foods, fast foods, fast foods industry, fast foods restaurant, frozen foods, deep frozen foods, fast frozen foods, quick frozen foods, dry frozen foods. (MSE)

Frozen shoulder and the Big Five personality traits.

PubMed

Debeer, Philippe; Franssens, Fien; Roosen, Isabelle; Dankaerts, Wim; Claes, Laurence

2014-02-01

In the past, several studies have suggested the existence of a "periarthritic personality" in patients with frozen shoulder. We conducted a study to determine differences in personality traits in patients with primary and secondary frozen shoulders. We prospectively evaluated 118 patients (84 women and 34 men; mean age, 53.8 years; SD 7.56) with a frozen shoulder. Of these patients, 48 had an idiopathic frozen shoulder and 70 had a secondary frozen shoulder. Personality traits were determined by the NEO Five-Factor Inventory (NEO-FFI) scale. This questionnaire measures the 5 major personality traits and is based on the norms determined in a neutral test situation for 2415 controls. Compared with healthy controls, no differences in personality traits were found in patients with primary and secondary frozen shoulder, except for Conscientiousness and Extraversion, for which patients with secondary frozen shoulder scored significantly higher than healthy controls. Patients with primary frozen shoulder scored significantly higher on Openness to Experience than did patients with secondary frozen shoulder; on the other 4 Big Five personality traits, no significant differences were found between patients with primary and secondary frozen shoulder. More specifically, patients with idiopathic frozen shoulder did not score higher on the trait Neuroticism as would be expected from previous publications. Our study results do not indicate that patients with an idiopathic frozen shoulder have a specific personality compared with healthy controls. Only a few differences were found in personality traits when the entire frozen shoulder group was compared with healthy controls and between patients with primary and secondary frozen shoulders. The results of this study suggest that these differences are not sufficient to speak about a specific "frozen shoulder personality." Copyright © 2014 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Mosby, Inc. All rights reserved.

Inner Core Imaging Using P'P'

NASA Astrophysics Data System (ADS)

Day, E. A.; Ward, J. A.; Bastow, I. D.; Irving, J. C. E.

2016-12-01

The Earth's inner core is a surprisingly complex region of our planet. Simple models of inner core solidification and evolution would lead us to expect a layered structure, which has "frozen in" in information about the state of the core at the time of solidification. However, seismic observations of Earth's inner core are not dominated by a radial "tree-ring" like pattern, but instead have revealed a hemispherical dichotomy in addition to depth dependent variations. There is a degree-one structure in isotropic and anisotropic velocities and in attenuation between the so-called eastern and western hemispheres of the inner core, with different depth distributions proposed for these varying phenomena. A range of mechanisms have been proposed to explain the hemispherical differences. These include models that require differences between the two hemispheres at the time of formation, post-solidification texturing, convection in the inner core, or hybrid mechanisms. Regional observations of the inner core suggest that a simple division between East and West may not be able to fully capture the structure present in the inner core. More detailed seismic observations will help us to understand the puzzle of the inner core's evolution. In this study we focus on updating observations of the seismic phase P'P', an inner core sensitive body wave with a more complex path than those typically used to study the inner core. By making new measurements of P'P' we illuminate new regions of the core with a high frequency phase that is sensitive to small scale structures. We examine the differential travel times of the different branches of P'P' (PKIKPPKIKP and PKPPKP), comparing the arrival time of inner core turning branch, P'P'df, with the arrival times of branches that turn in the outer core. P'P' is a relatively small amplitude phase, so we use both linear and non-linear stacking methods to make observations of the P'P' signals. These measurements are sensitive to the broad scale hemispherical pattern of anisotropy in the inner core as well as smaller scale variations.

The Eccentric Behavior of Nearly Frozen Orbits

NASA Technical Reports Server (NTRS)

Sweetser, Theodore H.; Vincent, Mark A.

2013-01-01

Frozen orbits are orbits which have only short-period changes in their mean eccentricity and argument of periapse, so that they basically keep a fixed orientation within their plane of motion. Nearly frozen orbits are those whose eccentricity and argument of periapse have values close to those of a frozen orbit. We call them "nearly" frozen because their eccentricity vector (a vector whose polar coordinates are eccentricity and argument of periapse) will stay within a bounded distance from the frozen orbit eccentricity vector, circulating around it over time. For highly inclined orbits around the Earth, this distance is effectively constant over time. Furthermore, frozen orbit eccentricity values are low enough that these orbits are essentially eccentric (i.e., off center) circles, so that nearly frozen orbits around Earth are bounded above and below by frozen orbits.

Structure and thermodynamics of a simple fluid

NASA Astrophysics Data System (ADS)

Stell, G.; Weis, J. J.

1980-02-01

Monte Carlo results are found for a simple fluid with a pair potential consisting of a hard-sphere core and a Lennard-Jones attractive tail. They are compared with several of the most promising recent theoretical treatments of simple fluids, all of which involve the decomposition of the pair potential into a hard-sphere-core term and an attractive-tail term. This direct comparison avoids the use of a second perturbation scheme associated with softening the core, which would introduce an ambiguity in the significance of the differences found between the theoretical and Monte Carlo results. The study includes the optimized random-phase approximation (ORPA) and exponential (EXP) approximations of Andersen and Chandler, an extension of the latter approximation to nodal order three (the N3 approximation), the linear-plus-square (LIN + SQ) approximation of Høye and Stell, the renormalized hypernetted chain (RHNC) approximation of Lado, and the quadratic (QUAD) approximation suggested by second-order self-consistent Γ ordering, the lowest order of which is identical to the ORPA. As anticipated on the basis of earlier studies, it is found that the EXP approximation yields radial distribution functions and structure factors of excellent overall accuracy in the liquid state, where the RHNC results are also excellent and the EXP, QUAD, and LIN + SQ results prove to be virtually indistinguishable from one another. For all the approximations, however, the thermodynamics from the compressibility relation are poor and the virial-theorem results are not uniformly reliable. Somewhat more surprisingly, it is found that the EXP results yield a negative structure factor S(k) for very small k in the liquid state and poor radial distribution functions at low densities. The RHNC results are nowhere worse than the EXP results and in some states (e.g., at low densities) much better. In contrast, the N3 results are better in some respects than the EXP results but worse in others. The authors briefly comment on the RHNC and EXP approximations applied to the full Lennard-Jones potential, for which the EXP approximation appears somewhat improved in the liquid state as a result of the softening of the potential core.

21 CFR 102.26 - Frozen “heat and serve” dinners.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Nonstandardized Foods § 102.26 Frozen “heat and serve” dinners. (a) A frozen “heat and serve” dinner: (1) Shall... consists of all of the following: (1) The phrase “frozen ‘heat and serve’ dinner,” except that the name of... precede the word “dinner” (e.g., “frozen chicken dinner” or “frozen heat and serve beef dinner”). The...

21 CFR 102.26 - Frozen “heat and serve” dinners.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Nonstandardized Foods § 102.26 Frozen “heat and serve” dinners. (a) A frozen “heat and serve” dinner: (1) Shall... consists of all of the following: (1) The phrase “frozen ‘heat and serve’ dinner,” except that the name of... precede the word “dinner” (e.g., “frozen chicken dinner” or “frozen heat and serve beef dinner”). The...

21 CFR 102.26 - Frozen “heat and serve” dinners.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Nonstandardized Foods § 102.26 Frozen “heat and serve” dinners. (a) A frozen “heat and serve” dinner: (1) Shall... consists of all of the following: (1) The phrase “frozen ‘heat and serve’ dinner,” except that the name of... precede the word “dinner” (e.g., “frozen chicken dinner” or “frozen heat and serve beef dinner”). The...

The Development of Alternative Food Cost Indexes

DTIC Science & Technology

1974-11-01

Frozen frufts, vegetables , fruit juices , and cake mixes are added and the quantity of purchased bread increased in this FCI. Comment was made that some... Grain , Frozen Vegetables , Mixed, Frozen Beans, Green, Frozen Peaches, Canned Apples, Fresh Oranges, Fresh Strawberries, Frozen Juice , Pineapple...also falls to recognize such accepted food service practices as the use of frozen fruits , vegetables , and juices , or prepared cake mixes, Tj,e

Identification of a dynamic temperature threshold for soil moisture freeze/thaw (F/T) state classification using soil real dielectric constant derivatives.

NASA Astrophysics Data System (ADS)

Pardo, R.; Berg, A. A.; Warland, J. S.

2017-12-01

The use of microwave remote sensing for surface ground ice detection has been well documented using both active and passive systems. Typical validation of these remotely sensed F/T state products relies on in-situ air or soil temperature measurements and a threshold of 0°C to identify frozen soil. However, in soil pores, the effects of capillary and adsorptive forces combine with the presence of dissolved salts to depress the freezing point. This is further confounded by the fact that water over this temperature range releases/absorbs latent heat of freezing/fusion. Indeed, recent results from SLAPEx2015, a campaign conducted to evaluate the ability to detect F/T state and examine the controls on F/T detection at multiple resolutions, suggest that using a soil temperature of 0°C as a threshold for freezing may not be appropriate. Coaxial impedance sensors, like Steven's HydraProbeII (HP), are the most widely used soil sensor in water supply forecast and climatological networks. These soil moisture probes have recently been used to validate remote sensing F/T products. This kind of validation is still relatively uncommon and dependent on categorical techniques based on seasonal reference states of frozen and non-frozen soil conditions. An experiment was conducted to identify the correlation between the phase state of the soil moisture and the probe measurements. Eight soil cores were subjected to F/T transitions in an environmental chamber. For each core, at a depth of 2.5 cm, the temperature and real dielectric constant (rdc) were measured every five minutes using HPs while two heat pulse probes captured the apparent heat capacity 24 minutes apart. Preliminary results show the phase transition of water is bounded by inflection points in the soil temperature, attributed to latent heat. The rdc, however, appears to be highly sensitive to changes in the water preceding the phase change. This opens the possibility of estimating a dynamic temperature threshold for soil F/T by identifying the soil temperatures at the times during which these inflection points in the soil rdc occur. This technique provides a more accurate threshold for F/T product than the static reference temperature currently established.

Laser ablation of dental tissues with picosecond pulses of 1.06-microm radiation transmitted through a hollow-core photonic-crystal fiber.

PubMed

Konorov, Stanislav O; Mitrokhin, Vladimir P; Fedotov, Andrei B; Sidorov-Biryukov, Dmitrii A; Beloglazov, Valentin I; Skibina, Nina B; Shcherbakov, Andrei V; Wintner, Ernst; Scalora, Michael; Zheltikov, Aleksei M

2004-04-10

Sequences of picosecond pulses of 1.06-microm Nd:YAG laser radiation with a total energy of approximately 2 mJ are transmitted through a hollow-core photonic-crystal fiber with a core diameter of approximately 14 microm and are focused onto a tooth's surface in vitro to ablate dental tissue. The hollow-core photonic-crystal fiber is shown to support the single-fundamental-mode regime for 1.06-microm laser radiation, serving as a spatial filter and allowing the laser beam's quality to be substantially improved. The same fiber is used to transmit emission from plasmas produced by laser pulses onto the tooth's surface in the backward direction for detection and optical diagnostics.

TOPEX/Poseidon orbit maintenance for the first five years

NASA Technical Reports Server (NTRS)

Bhat, R. S.; Shapiro, B. E.; Frauenholz, R. B.; Leavitt, R. K.

1998-01-01

The TOPEX/Poseidon orbit maintenance strategy was changed following launch to include the effects of observed unmodeled, and hence anomalous, along-track accelerations. The anomalous force causes the semi-major axis, a, to either increase (called "boost") or decrease ("deboost" or "decay") depending on the satellite attitude and solar array pitch angle offset. Although this force is the most uncertain parameter in ground track prediction, it has been used as a passive technique for orbit maintenance, thereby reducing the number of propulsive maneuvers, enhancing maneuver spacing, and to place maneuvers at convenient times. This passive technique was first demonstrated in May 1993. The TOPEX/Poseidon orbit has been uniquely maintained using both passive (non-propulsive) and active (propulsive) maneuvers. Furthermore, the orbit has been maintained using only the passive technique since the ninth orbit maintenance maneuver on January 15, 1996. Only nine orbit maintenance maneuvers have been required to maintain the ground track, including verification site over flights, since achieving the operational orbit on September 21, 1992 (mission requirement: 95% within +/- l km). During this period, a has varied within 7,714,429 +/- 7 m, while the inclination i periodically fluctuated in the range 66.0408 deg. +/- 0.0040 deg. The frozen orbit (required e < 0.001 and omega approximately equals to 90 deg.) has been maintained without any dedicated eccentricity maneuvers. The frozen eccentricity vector has completed two periodic cycles and it is currently tracing its third cycle (period approximately equals 26.7 months).

Particle distributions in approximately 10(14) 10(16) eV air shower cores at sea level

NASA Technical Reports Server (NTRS)

Hodson, A. L.; Ash, A. G.; Bull, R. M.

1985-01-01

Experimental evidence is reported for fixed distances (0, 1.0, 2.5 and 4.0 m) from the shower centers and for core flattening. The cores become flatter, on average, as the shower size (primary energy) increases. With improved statistics on 4192 cores, the previous results are exactly confirmed.

Distribution of collagens type V and VI in the normal human alveolar mucosa: an immunoelectronmicroscopic study using ultrathin frozen sections.

PubMed

Rabanus, J P; Gelderblom, H R; Schuppan, D; Becker, J

1991-05-01

The ultrastructural localization of collagens type V and VI in normal human gingival mucosa was investigated by immunoelectron microscopy. Twenty biopsies were fixed in dimethylsuberimidate and shock-frozen in slush nitrogen. Collagen type V was mainly located to meshworks of uniform nonstriated microfibrils of 12 to 20 nm width, which preferentially appeared in larger spaces between cross-striated major collagen fibrils. Occasionally single microfibrils of collagen type V fanned out from the ends of major collagen fibrils, which may indicate a role as a core fibril. Collagen type V was not found in the subepithelial basement membrane and the immediately adjacent stroma. Collagen type VI was detected in a loose reticular network of unbanded microfilaments that were morphologically distinguishable by knoblike protrusions every 100-110 nm. These microfilaments were found in the vicinity, but not as an intrinsic component, of the subepithelial basement membrane. Single filaments of collagen type VI filaments appeared to form bridges between neighboring cross-striated major collagen fibrils, suggesting an interconnecting role for this collagen type. The method presented appears to be excellently suited to study the normal and pathological supramolecular organization of the oral extracellular matrix.

Comparison of dynamical approximation schemes for nonlinear gravitaional clustering

NASA Technical Reports Server (NTRS)

Melott, Adrian L.

1994-01-01

We have recently conducted a controlled comparison of a number of approximations for gravitational clustering against the same n-body simulations. These include ordinary linear perturbation theory (Eulerian), the lognormal approximation, the adhesion approximation, the frozen-flow approximation, the Zel'dovich approximation (describable as first-order Lagrangian perturbation theory), and its second-order generalization. In the last two cases we also created new versions of the approximation by truncation, i.e., by smoothing the initial conditions with various smoothing window shapes and varying their sizes. The primary tool for comparing simulations to approximation schemes was cross-correlation of the evolved mass density fields, testing the extent to which mass was moved to the right place. The Zel'dovich approximation, with initial convolution with a Gaussian e(exp -k(exp 2)/k(sub G(exp 2)), where k(sub G) is adjusted to be just into the nonlinear regime of the evolved model (details in text) worked extremely well. Its second-order generalization worked slightly better. We recommend either n-body simulations or our modified versions of the Zel'dovich approximation, depending upon the purpose. The theoretical implication is that pancaking is implicit in all cosmological gravitational clustering, at least from Gaussian initial conditions, even when subcondensations are present. This in turn provides a natural explanation for the presence of sheets and filaments in the observed galaxy distribution. Use of the approximation scheme can permit extremely rapid generation of large numbers of realizations of model universes with good accuracy down to galaxy group mass scales.

Numerical Study on the Validity of the Taylor Hypothesis in Space Plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Perri, Silvia; Servidio, Sergio; Valentini, Francesco

In situ heliospheric measurements allow us to resolve fluctuations as a function of frequency. A crucial point is to describe the power spectral density as a function of the wavenumber, in order to understand the energy cascade through the scales in terms of plasma turbulence theories. The most favorable situation occurs when the average wind speed is much higher than the phase speed of the plasma modes, equivalent to the fact that the fluctuations’ dynamical times are much longer than their typical crossing period through the spacecraft (frozen-in Taylor approximation). Using driven compressible Hall-magneothydrodynamics simulations, in which an “imaginary” spacecraftmore » flies across a time-evolving turbulence, here we explore the limitations of the frozen-in assumption. We find that the Taylor hypothesis is robust down to sub-proton scales, especially for flows with mean velocities typical of the fast solar wind. For slow mean flows (i.e., speeds of the order of the Alfvèn speed) power spectra are subject to an amplitude shift throughout the scales. At small scales, when dispersive decorrelation mechanisms become significant, the frozen-in assumption is generally violated, in particular for k -vectors almost parallel to the average magnetic field. A discussion in terms of the spacetime autocorrelation function is proposed. These results might be relevant for the interpretation of the observations, in particular for existing and future space missions devoted to very high-resolution measurements.« less

78 FR 45907 - United States Standards for Grades of Frozen Vegetables

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-30

... standards covered by these revisions are: frozen asparagus, frozen lima beans, frozen speckled butter beans..., lima beans, speckled butter beans, cooked squash, summer squash, etc., and not other vegetables. AMS... document. United States Standards for Grades of Frozen Lima Beans Update address for AMS. Change ``U.S...

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false âFresh,â âfreshly frozen,â âfresh frozen,â âfrozen fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly...

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 2 2013-04-01 2013-04-01 false âFresh,â âfreshly frozen,â âfresh frozen,â âfrozen fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly...

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 2 2012-04-01 2012-04-01 false âFresh,â âfreshly frozen,â âfresh frozen,â âfrozen fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly...

Cryopreservation of Autologous Blood (Red Blood Cells, Platelets and Plasma)

NASA Astrophysics Data System (ADS)

Ebine, Kunio

Prevention of post-transfusion hepatitis is still a problem in cardiovascular surgery. We initiated the cryopreservation of autologous blood for the transfusion in elective cardiovascular surgery since 1981. This study includes 152 surgical cases in which autologous frozen, allogeneic frozen, and/or allogeneic non-frozen blood were used. In the 152 surgical cases, there were 69 cases in which autologous blood only (Group I) was used; 12 cases with autologous and allogeneic frozen blood (Group II); 46 cases with autologous and allgeneic frozen plus allogeneic non-frozen blood (Group III); and 25 cases with allogeneic frozen plus allogeneic non-frozen blood (Group IV). No hepatitis developed in Groups I (0%) and II (0%), but there was positive hepatitis in Groups III (4.3%) and IV (8.0%) . In 357 cases of those who underwent surgery with allogeneic non-frozen whole blood during the same period, the incidence rate of hepatitis was 13.7% (49/357). Patients awaiting elective surgery can store their own blood in the frozen state. Patients who undergo surgery with the cryoautotransfusion will not produce any infections or immunologic reactions as opposed to those who undergo surgery with the allogeneic non-frozen blood.

The radio emission from the ultraluminous far-infrared galaxy NGC 6240

NASA Technical Reports Server (NTRS)

Colbert, Edward J. M.; Wilson, Andrew S.; Bland-Hawthorn, Jonathan

1994-01-01

We present new radio observations of the 'prototypical' ultraluminous far-infrared galaxy NGC 6240, obtained using the Very Large Array (VLA) at lambda = 20 cm in B-configuration and at lambda = 3.6 cm in A-configuration. These data, along with those from four previous VLA observations, are used to perform a comprehensive study of the radio emission from NGC 6240. Approximately 70% (approximately 3 x 10(exp 23) W/Hz) of the total radio power at 20 cm originates from the nuclear region (approximately less than 1.5 kpc), of which half is emitted by two unresolved (R approximately less than 36 pc) cores and half by a diffuse component. The radio spectrum of the nuclear emission is relatively flat (alpha approximately equals 0.6; S(sub nu) proportional to nu(exp -alpha). The supernova rate required to power the diffuse component is consistent with that predicted by the stellar evolution models of Rieke et al. (1985). If the radio emission from the two compact cores is powered by supernova remnants, then either the remnants overlap and form hot bubbles in the cores, or they are very young (approximately less than 100 yr.) Nearly all of the remaining 30% of the total radio power comes from an 'armlike' region extending westward from the nuclear region. The western arm emission has a steep spectrum (alpha approximately equals 1.0), suggestive of aging effects from synchrotron or inverse-Compton losses, and is not correlated with starlight; we suggest that it is synchrotron emission from a shell of material driven by a galactic superwind. Inverse Compton scattering of far-infrared photons in the radio sources is expected to produce an X-ray flux of approximately 2 - 6 x 10(exp -14) ergs/s/sq cm in the 2 - 10 keV band. No significant radio emission is detected from or near the possible ultramassive 'dark core'.

DRAQ5 and Eosin (‘D&E’) as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues

PubMed Central

Elfer, Katherine N.; Sholl, Andrew B.; Wang, Mei; Tulman, David B.; Mandava, Sree H.; Lee, Benjamin R.; Brown, J. Quincy

2016-01-01

Real-time on-site histopathology review of biopsy tissues at the point-of-procedure has great potential for significant clinical value and improved patient care. For instance, on-site review can aid in rapid screening of diagnostic biopsies to reduce false-negative results, or in quantitative assessment of biospecimen quality to increase the efficacy of downstream laboratory and histopathology analysis. However, the only currently available rapid pathology method, frozen section analysis (FSA), is too time- and labor-intensive for use in screening large quantities of biopsy tissues and is too destructive for maximum tissue conservation in multiple small needle core biopsies. In this work we demonstrate the spectrally-compatible combination of the nuclear stain DRAQ5 and the anionic counterstain eosin as a dual-component fluorescent staining analog to hematoxylin and eosin intended for use on fresh, unsectioned tissues. Combined with optical sectioning fluorescence microscopy and pseudo-coloring algorithms, DRAQ5 and eosin (“D&E”) enables very fast, non-destructive psuedohistological imaging of tissues at the point-of-acquisition with minimal tissue handling and processing. D&E was validated against H&E on a one-to-one basis on formalin-fixed paraffin-embedded and frozen section tissues of various human organs using standard epi-fluorescence microscopy, demonstrating high fidelity of the staining mechanism as an H&E analog. The method was then applied to fresh, whole 18G renal needle core biopsies and large needle core prostate biospecimen biopsies using fluorescence structured illumination optical sectioning microscopy. We demonstrate the ability to obtain high-resolution histology-like images of unsectioned, fresh tissues similar to subsequent H&E staining of the tissue. The application of D&E does not interfere with subsequent standard-of-care H&E staining and imaging, preserving the integrity of the tissue for thorough downstream analysis. These results indicate that this dual-stain pseudocoloring method could provide a real-time histology-like image at the time of acquisition and valuable objective tissue analysis for the clinician at the time of service. PMID:27788264

Effects of frozen storage on survival of Staphylococcus aureus and enterotoxin production in precooked tuna meat.

PubMed

Wu, Xulei; Su, Yi-Cheng

2014-08-01

This study investigated the survival of Staphylococcus aureus in precooked tuna meat for producing canned products during frozen storage (-20 ± 2 °C) as well as its growth and enterotoxin production at 35 to 37 °C after the storage. Samples (50 ± 5 g) of precooked albacore (loin, chunk, and flake) and skipjack (chunk and flake) tuna were inoculated with 5 enterotoxin-producing strains of S. aureus at a level of approximately 3.5 log CFU/g and individually packed in a vacuum bag after 3 h incubation at 35 to 37 °C. Vacuum-packed samples were stored in a freezer (-20 ± 2 °C) for 4 wk. The frozen samples were then thawed in 37 °C circulating water for 2 h and incubated at 35 to 37 °C for 22 h. Populations of S. aureus in all precooked tuna samples decreased slightly (<0.7 log CFU/g) after 4 wk of storage at -20 ± 2 °C, but increased rapidly once the samples were thawed and held at 35 to 37 °C. Total S. aureus counts in albacore and skipjack samples increased by greater than 3 log CFU/g after 6 and 8 h of exposure to 35 to 37 °C, respectively. All samples became spoiled after 10 h of exposure to 35 to 37 °C, while no enterotoxin was detected in any samples. However, enterotoxins were detected in albacore loin and other samples after 12 and 24 h of incubation at 35 to 37 °C, respectively. Frozen precooked tuna meat should be used for producing canned tuna within 6 to 8 h of thawing to avoid product spoilage and potential enterotoxin production by S. aureus in contaminated precooked tuna meat. © 2014 Institute of Food Technologists®

Conservation science in a terrorist age: the impact of airport security screening on the viability and DNA integrity of frozen felid spermatozoa.

PubMed

Gloor, Kayleen T; Winget, Doug; Swanson, William F

2006-09-01

In response to growing terrorism concerns, the Transportation Security Administration now requires that all checked baggage at U.S. airports be scanned through a cabinet x-ray system, which may increase risk of radiation damage to transported biologic samples and other sensitive genetic material. The objective of this study was to investigate the effect of these new airport security regulations on the viability and DNA integrity of frozen felid spermatozoa. Semen was collected from two domestic cats (Felis silvestris catus) and one fishing cat (Prionailurus viverrinus), cryopreserved in plastic freezing straws, and transferred into liquid nitrogen dry shippers for security screening. Treatment groups included frozen samples from each male scanned once or three times using a Transportation Security Administration-operated cabinet x-ray system, in addition to non-scanned samples (i.e., negative control) and samples previously scanned three times and exposed to five additional high-intensity x-ray bursts (i.e., positive control). Dosimeters placed in empty dry shippers were used to quantify radiation exposure. Following treatment, straws were thawed and spermatozoa analyzed for post-thaw motility (percentage motile and rate of progressive movement), acrosome status, and DNA integrity using single-cell gel electrophoresis (i.e., the comet assay). Dosimeter measurements determined that each airport screening procedure produced approximately 16 mrem of radiation exposure. Our results indicated that all levels of radiation exposure adversely affected (P < 0.05) post-thaw sperm motility, but the percentage of acrosome-intact spermatozoa did not differ (P > 0.05) among treatment groups. Results also showed that the amount of double-stranded DNA damage was greater (P < 0.05) in sperm samples from both cat species scanned three times compared to samples scanned once or negative controls. Findings suggest that new airport security measures may cause radiation-induced damage to frozen spermatozoa and other valuable biologic samples transported on passenger aircraft and that alternative modes of sample transportation should be used whenever possible.

Restaurant Policies and Practices for Serving Raw Fish in Minnesota.

PubMed

Hedeen, Nicole

2016-10-01

The number of restaurants serving sushi within Minnesota is continuously increasing. The practices and protocols of serving raw fish are complex and require detailed planning to ensure that food served to patrons will not cause illness. Although the popularity of sushi is increasing, there is a lack of research on food safety issues pertaining to preparation of raw fish and sushi rice. To address this gap, the Minnesota Department of Health Environmental Health Specialists Network Food program collected descriptive data on restaurant practices and policies concerning the service of raw fish and sushi rice in 40 Minnesota restaurants. At each restaurant, a specialist interviewed a restaurant manager, conducted an observation of the sushi prep areas in the restaurant kitchen, and reviewed parasite destruction letters and invoices from fish supplier(s). Over half of the restaurants (59%) were missing one or more of the parasite destruction letters from their fish supplier(s) guaranteeing that fish had been properly frozen to the time and temperature requirements in the Minnesota Food Code. A total of 42 parasite destruction letters from suppliers were observed; 10% were considered "adequate" letters. The majority of the letters were missing details pertaining to the types of fish frozen, the length of time fish were frozen, or details on what temperatures fish were held frozen or a combination of all three. Most restaurants were using time as a public health control for their sushi rice. For those restaurants using time as a public health control, 26% had a written procedure on-site, and approximately 53% were keeping track of time. Bare hand contact during sushi prep was observed in 17% of restaurants, and in more than 40% of the restaurants, at least one fish was mislabeled on the menu. Findings from this study indicate that many Minnesota restaurants are not complying with the Food Code requirements pertaining to parasite destruction for the service of raw fish or the use of time as a public health control for sushi rice.

Short-term Clinical Results of Manipulation Under Ultrasound-Guided Brachial Plexus Block in Patients with Idiopathic Frozen Shoulder and Diabetic Secondary Frozen Shoulder.

PubMed

Ando, Akira; Hamada, Junichiro; Hagiwara, Yoshihiro; Sekiguchi, Takuya; Koide, Masashi; Itoi, Eiji

2018-01-01

This study examined the effectiveness of manipulation under ultrasound-guided brachial plexus block in patients with recalcitrant idiopathic frozen shoulder and diabetic secondary frozen shoulder (diabetic frozen shoulder). Forty-four idiopathic frozen shoulders and 10 diabetic frozen shoulders with failed conservative treatment for at least 3 months were included in this study. The manipulation was performed under ultrasound-guided brachial plexus block and visual analogue scale, range of motion, and Constant scores were measured before manipulation and at the last follow-up examination. No major complications were observed during the procedure. Sufficient improvement was not obtained in two patients during the procedure and to avoid complications, the procedure was discontinued and subsequently arthroscopic capsular release was performed. Visual analogue scale, range of motion towards all directions, and Constant scores were significantly improved after the manipulation in both the idiopathic frozen shoulder and diabetic frozen shoulder groups, however the diabetic group showed inferior results compared with those of the idiopathic group. This manipulation was effective and shortened the duration of symptoms in most of the idiopathic and diabetic frozen shoulders without major complications during the procedure. Diabetic frozen shoulder showed inferior clinical results and difficulty in recovery in range of motion, which indicated that diabetic frozen shoulder should be discussed as a different entity.

21 CFR 160.150 - Frozen egg whites.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen egg whites. 160.150 Section 160.150 Food... HUMAN CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.150 Frozen egg whites. (a) Frozen egg whites, frozen egg albumen is the food prepared by freezing...

21 CFR 160.190 - Frozen egg yolks.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen egg yolks. 160.190 Section 160.190 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.190 Frozen egg yolks. (a) Frozen egg yolks, frozen yolks is the food prepared by freezing egg yolks that...

21 CFR 160.190 - Frozen egg yolks.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen egg yolks. 160.190 Section 160.190 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.190 Frozen egg yolks. (a) Frozen egg yolks, frozen yolks is the food prepared by freezing egg yolks that...

21 CFR 160.150 - Frozen egg whites.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen egg whites. 160.150 Section 160.150 Food... HUMAN CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.150 Frozen egg whites. (a) Frozen egg whites, frozen egg albumen is the food prepared by freezing...

Assessing the Rationale and Effectiveness of Frozen Plasma Transfusions: An Evidence-based Review.

PubMed

Tinmouth, Alan

2016-06-01

Frozen plasma is a commonly used blood product. The primary indications for frozen plasma are the treatment and prevention of bleeding in patients with prolonged coagulation tests. However, there is a lack of well-conducted clinical trials to determine the appropriate indications for frozen plasma. The rationale and evidence for frozen plasma transfusions are reviewed, including the evidence or lack of evidence supporting common indications. Targeting indications in which frozen plasma transfusions are clearly not beneficial as supported by the current evidence provides an opportunity to improve the current use of frozen plasma and reduce adverse transfusion events. Copyright © 2016 Elsevier Inc. All rights reserved.

Do Supernovae Make or Kill Pulsars?

NASA Astrophysics Data System (ADS)

Geppert, U.; Page, D.; Zannias, T.

1998-12-01

The effect of post core-collapse accretion on the magnetic field (MF) of a new born neutron star (NS) is considered. If this accretion is hypercritical than any initially in the NS matter frozen in MF will be submerged beneath the accreted matter. If the accreted matter is non magnetized, NS produced by SN in which hypercritical accretion occured are born with weak surface MF. This mechanism may contribute to the deficit of observed PSR in SNR and may also explain the discrepancy between the estimated PSR birthrate and type Ib + II SN rates. The dependence of the re-diffusion of the submerged MF on the fall-back accretion is discussed too.

Laboratory experiments on interstellar ice analogs: The sticking and desorption of small physisorbed molecules

NASA Technical Reports Server (NTRS)

Fuchs, G. W.; Acharyya, K.; Bisschop, S. E.; Oberg, K. I.; vanBroekhuizen, F. A.; Fraser, H. J.; Schlemmer, S.; vanDishoeck, E. F.; Linnartz, H.

2006-01-01

Molecular oxygen and nitrogen are difficult to observe since they are infrared inactive and radio quiet. The low O2 abundances found so far combined with general considerations of dense cloud conditions suggest molecular oxygen is frozen out at low temperatures (< 20 K) in the shielded inner regions of cloud cores. In solid form O2 and N2 can only be observed as adjuncts within other ice constituents, like CO. In this work we focus on fundamental properties of N2 and O2 in CO ice-gas systems, e.g. desorption characteristics and sticking probabilities at low temperatures for different ice morphologies.

Longitudinal residual stresses in boron fibers

NASA Technical Reports Server (NTRS)

Behrendt, D. R.

1976-01-01

A method of measuring the longitudinal residual stress distribution in boron fibers is presented. The residual stresses in commercial CVD boron on tungsten fibers of 102, 142, and 203 microns (4, 5.6, and 8 mil) diameters were determined. Results for the three sizes show a compressive stress at the surface 800 to -1400 MN/sq m 120 to -200 ksi), changing monotonically to a region of tensile stress within the boron. At approximately 25 percent of the original radius, the stress reaches a maximum tensile 600 to 1000 MN/sq m(90 to 150 ksi) and then decreases to compressive near the tungsten boride core. The core itself is under a compressive stress of approximately -1300 MN/sq m (-190 ksi). The effects of surface removal on core residual stress and core-initiated fracture are discussed.

Frozen Section Evaluation of Margin Status in Primary Squamous Cell Carcinomas of the Head and Neck: A Correlation Study of Frozen Section and Final Diagnoses.

PubMed

Layfield, Eleanor M; Schmidt, Robert L; Esebua, Magda; Layfield, Lester J

2018-06-01

Frozen section is routinely used for intraoperative margin evaluation in carcinomas of the head and neck. We studied a series of frozen sections performed for margin status of head and neck tumors to determine diagnostic accuracy. All frozen sections for margin control of squamous carcinomas of the head and neck were studied from a 66 month period. Frozen and permanent section diagnoses were classified as negative or malignant. Correlation of diagnoses was performed to determine accuracy. One thousand seven hundred and ninety-six pairs of frozen section and corresponding permanent section diagnoses were obtained. Discordances were found in 55 (3.1%) pairs. In 35 pairs (1.9%), frozen section was reported as benign, but permanent sections disclosed carcinoma. In 21 cases, the discrepancy was due to sampling and in the remaining cases it was an interpretive error. In 20 cases (1.1%), frozen section was malignant, but the permanent section was interpreted as negative. Frozen section is an accurate method for evaluation of operative margins for head and neck carcinomas with concordance between frozen and permanent results of 97%. Most errors are false negative results with the majority of these being due to sampling issues.

Development of the Soil Moisture Active Passive (SMAP) radiometer derived landscape freeze/thaw product

NASA Astrophysics Data System (ADS)

Colliander, A.; Xu, X.; Dunbar, R. S.; Derksen, C.; Kim, Y.; Kimball, J. S.

2016-12-01

A baseline SMAP mission objective was to determine the land surface binary freeze/thaw (FT) state for northern (>45°N) regions with 80% spatial classification accuracy at 3 km resolution and 2-day average intervals. These requirements were initially achieved from the SMAP radar until the sensor failed in July 2015. The FT algorithm is now transitioning to using SMAP radiometer inputs. The main compromises of this change are a coarse (36 km) radiometer footprint, enhanced noise and potential FT signal degradation from seasonal vegetation biomass, soil moisture and surface inundation changes. The new daily passive FT product (L3_FT_P) is based on the same seasonal threshold algorithm as the radar derived product (L3_FT_A): instantaneous SMAP measurements are compared to reference signatures acquired during seasonal frozen and thawed states. Instead of radar inputs, the normalized polarization ratio (NPR) is calculated from SMAP radiometer measurements. The L3_FT_P algorithm is applied using NPR inputs, whereby NPR decreases and increases are associated with respective landscape freezing and thawing. A lower NPR under frozen conditions is due to smaller V-pol brightness temperature increases and larger H-pol increases. Using in situ measurements from core validation sites, the temporal behavior of backscatter and NPR measurements were evaluated during the spring 2015 radar and radiometer overlap period. The transition from frozen to thawed states produced a NPR response similar in timing and magnitude to the radar response, resulting in similar freeze to thaw seasonal transition dates. While the post-thaw radar backscatter consistently remained at elevated values relative to the frozen state, the NPR drifted downwards following the main thaw transition (due to de-polarization of the scene), which may introduce false freeze classification errors. Both radar and radiometer results tended to lead observed soil thawing due to strong sensitivity of the microwave retrievals to wet snow. Continued analysis of SMAP radiometer measurements will help to identify different landscape components of the SMAP freeze-thaw temporal signal.

21 CFR 161.175 - Frozen raw breaded shrimp.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen raw breaded shrimp. 161.175 Section 161.175... § 161.175 Frozen raw breaded shrimp. (a) Frozen raw breaded shrimp is the food prepared by coating one..., other than those provided for in this paragraph, are not suitable ingredients of frozen raw breaded...

21 CFR 161.175 - Frozen raw breaded shrimp.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen raw breaded shrimp. 161.175 Section 161.175... § 161.175 Frozen raw breaded shrimp. (a) Frozen raw breaded shrimp is the food prepared by coating one..., other than those provided for in this paragraph, are not suitable ingredients of frozen raw breaded...

Introducing a new methodology for the calculation of local philicity and multiphilic descriptor: an alternative to the finite difference approximation

NASA Astrophysics Data System (ADS)

Sánchez-Márquez, Jesús; Zorrilla, David; García, Víctor; Fernández, Manuel

2018-07-01

This work presents a new development based on the condensation scheme proposed by Chamorro and Pérez, in which new terms to correct the frozen molecular orbital approximation have been introduced (improved frontier molecular orbital approximation). The changes performed on the original development allow taking into account the orbital relaxation effects, providing equivalent results to those achieved by the finite difference approximation and leading also to a methodology with great advantages. Local reactivity indices based on this new development have been obtained for a sample set of molecules and they have been compared with those indices based on the frontier molecular orbital and finite difference approximations. A new definition based on the improved frontier molecular orbital methodology for the dual descriptor index is also shown. In addition, taking advantage of the characteristics of the definitions obtained with the new condensation scheme, the descriptor local philicity is analysed by separating the components corresponding to the frontier molecular orbital approximation and orbital relaxation effects, analysing also the local parameter multiphilic descriptor in the same way. Finally, the effect of using the basis set is studied and calculations using DFT, CI and Möller-Plesset methodologies are performed to analyse the consequence of different electronic-correlation levels.

The diamagnetic susceptibility of a donor in a semiconductor core shell quantum dot

NASA Astrophysics Data System (ADS)

Sudharshan, M. S.; Subhash, P.; Shaik, Nagoor Babu; Kalpana, P.; Jayakumar, K.; Reuben, A. Merwyn Jasper D.

2015-06-01

The effect of Aluminium concentration, shell thickness and size of the core shell Quantum Dot on the Diamagnetic Susceptibility of a donor in the Core Shell Quantum Dot is calculated in the effective mass approximation using the variational method. The results are presented and discussed.

Cassini radar views the surface of Titan.

PubMed

Elachi, C; Wall, S; Allison, M; Anderson, Y; Boehmer, R; Callahan, P; Encrenaz, P; Flamini, E; Franceschetti, G; Gim, Y; Hamilton, G; Hensley, S; Janssen, M; Johnson, W; Kelleher, K; Kirk, R; Lopes, R; Lorenz, R; Lunine, J; Muhleman, D; Ostro, S; Paganelli, F; Picardi, G; Posa, F; Roth, L; Seu, R; Shaffer, S; Soderblom, L; Stiles, B; Stofan, E; Vetrella, S; West, R; Wood, C; Wye, L; Zebker, H

2005-05-13

The Cassini Titan Radar Mapper imaged about 1% of Titan's surface at a resolution of approximately 0.5 kilometer, and larger areas of the globe in lower resolution modes. The images reveal a complex surface, with areas of low relief and a variety of geologic features suggestive of dome-like volcanic constructs, flows, and sinuous channels. The surface appears to be young, with few impact craters. Scattering and dielectric properties are consistent with porous ice or organics. Dark patches in the radar images show high brightness temperatures and high emissivity and are consistent with frozen hydrocarbons.

A direct measurement of the charge states of energetic iron emitted by the sun

NASA Technical Reports Server (NTRS)

Gloeckler, G.; Sciambi, R. K.; Fan, C. Y.; Hovestadt, D.

1976-01-01

The charge states of energetic iron have been measured directly for the first time in a solar particle event. In the energy interval 0.01 to 0.25 MeV per nucleon, iron is not fully stripped but has a mean ionization state of 11.6. This value is remarkably similar to the mean ionization state of iron in the quiet solar wind and suggests that the charge states were "frozen-in" at a coronal temperature of approximately 1,500,000 K.

Cryo-transmission electron microscopy of frozen-hydrated sections of Escherichia coli and Pseudomonas aeruginosa.

PubMed

Matias, Valério R F; Al-Amoudi, Ashraf; Dubochet, Jacques; Beveridge, Terry J

2003-10-01

High-pressure freezing of Escherichia coli K-12 and Pseudomonas aeruginosa PAO1 in the presence of cryoprotectants provided consistent vitrification of cells so that frozen-hydrated sections could be cut, providing approximately 2-nm resolution of structure. The size and shape of the bacteria, as well as their surface and cytoplasmic constituents, were nicely preserved and compared well with other published high-resolution techniques. Cells possessed a rich cytoplasm containing a diffuse dispersion of ribosomes and genetic material. Close examination of cells revealed that the periplasmic space was compressed during cryosectioning, a finding which provided supporting evidence that this space is filled by a compressible gel. Since the outer membrane and peptidoglycan layer are bonded together via lipoproteins, the space between them (although still part of the periplasmic space) was not as compacted. Even when this cryosectioning compression was taken into account, there was still substantial variability in the width of the periplasmic space. It is possible that the protoplast has some capacity to float freely within the periplasm.

Leakage of fluid around endotracheal tube cuffs: a cadaver study

PubMed Central

Lucius, Ralph; Ewald, Kristian

2013-01-01

Background The aim of the study was to evaluate the leakage of liquid past the cuffs of tracheal tubes in fresh frozen human heads. Methods Six truncated fresh frozen heads were used and intubated with 8.0 mm endotracheal tubes. The intracuff pressures tested were 30 and 100 cmH2O. Subsequently, 20 ml of each of two oral antiseptic rinses (0.2% chlorhexidine and octenidine [octenidol®, Schülke & Mayr GmbH, Norderstedt, Germany]) was applied for thirty seconds in the mouth. During the trial, leakage of the cuffs was examined. Results The sealing between the tracheal cuff and tracheal wall was leakage-proof for all tested intracuff pressures and all tested antiseptic rinses. However, approximately 5.6 ml and 1.8 ml leaked into the esophagus and remained as a cuff-puddle, respectively. Conclusions The sealing between an endotracheal tube cuff with an intracuff pressure of 30 cmH2O and the tracheal wall is leakage-proof during oral care with antiseptic rinsing. An increase of intracuff pressure to 100 cmH2O does not appear to be required. PMID:24363847

Frozen lattice and absorptive model for high angle annular dark field scanning transmission electron microscopy: A comparison study in terms of integrated intensity and atomic column position measurement.

PubMed

Alania, M; Lobato, I; Van Aert, S

2018-01-01

In this paper, both the frozen lattice (FL) and the absorptive potential (AP) approximation models are compared in terms of the integrated intensity and the precision with which atomic columns can be located from an image acquired using high angle annular dark field (HAADF) scanning transmission electron microscopy (STEM). The comparison is made for atoms of Cu, Ag, and Au. The integrated intensity is computed for both an isolated atomic column and an atomic column inside an FCC structure. The precision has been computed using the so-called Cramér-Rao Lower Bound (CRLB), which provides a theoretical lower bound on the variance with which parameters can be estimated. It is shown that the AP model results into accurate measurements for the integrated intensity only for small detector ranges under relatively low angles and for small thicknesses. In terms of the attainable precision, both methods show similar results indicating picometer range precision under realistic experimental conditions. Copyright © 2017 Elsevier B.V. All rights reserved.

Influence of water table on carbon dioxide, carbon monoxide, and methane fluxes from taiga bog microcosms

DOE Office of Scientific and Technical Information (OSTI.GOV)

Funk, D.W.; Pullmann, E.R.; Peterson, K.M.

1994-09-01

Hydrological changes, particularly alterations in water table level, may largely overshadow the more direct effects of global temperature increase upon carbon cycling in arctic and subarctic wetlands. Frozen cores (n=40) of intact soils and vegetation were collected from a bog near Fairbanks, Alaska, and fluxes of CO{sub 2}, CH{sub 4}, and Co in response to water table variation were studied under controlled conditions in the Duke University phytotron. Core microcosms thawed to a 20-cm depth over 30 days under a 20 hour photoperiod with a day/night temperature regime of 20/10{degrees}C. After 30 days the water table in 20 microcosms wasmore » decreased from the soil surface to -15 cm and maintained at the soil surface in 20 control cores. Outward fluxes of CO{sub 2} (9-16 g m{sup -2}d{sup -1}) and CO (3-4 mg m{sup -2}d{sup -1}) were greatest during early thaw and decreased to near zero for both gases before the water table treatment started. Lower water table tripled CO{sub 2} flux to the atmosphere when compared with control cores. Carbon monoxide was emitted at low rates from high water table cores and consumed by low water table cores. Methane fluxes were low (<1 mg m{sup -2}d{sup -1}) in all cores during thaw. High water table cores increased CH{sub 4} flux to 8-9 mg m{sup -2}d{sup -1} over 70 days and remained high relative to the low water table cores (<0.74 mg m{sup -2}d{sup -1}). Although drying of wetland taiga soils may decrease CH{sub 4} emissions to the atmosphere, the associated increase in CO{sub 2} due to aerobic respiration will likely increase the global warming potential of gas emissions from these soils. 43 refs., 4 figs.« less

Keeping Enceladus Warm

NASA Astrophysics Data System (ADS)

Travis, B. J.; Schubert, G.

2012-12-01

Despite its small size, Enceladus emits considerable heat, especially at its south pole, even long after simple thermal models predict it should be frozen. A number of energy mechanisms have been proposed as responsible for this heating, such as TDH (tidal dissipative heating), and convection and shearing in the ice shell, but why energy outflow is primarily at the south pole is still debated. It is not known if TDH has operated continuously at Enceladus. Crater relaxation simulations suggest considerable heat flow has occurred over long stretches of its history. One process missing from previous models is fluid flow, both in an ocean layer and in the silicate core. The simulations described here are part of a study to estimate the impact of hydrothermal flow and to explore under what conditions, and for how long, an ocean layer could persist on Enceladus, with or without TDH. Our model geometry is 2-D spherical (radius and latitude) for most simulations, with one 3-D spherical simulation. We assume a silicate core of about 160 km radius, overlain by an H2O layer out to 250 km radius. Ice shell thickness is initially 15 km. Flow in an ocean layer is represented by a simplified Navier-Stokes model, and porous flow occurs in the core. Surface temperature distribution follows observed values. Radiogenic heating produces about 0.3 GW in the model. A simple TDH model is active in some simulations. Salts and/or NH3 may be present in the interior of Enceladus, and would strongly depress freezing; our model uses a low eutectic salt as an analog. The ice shell's thickness is not required to remain fixed, but can change dynamically, in response to local thermodynamics. Initial core temperature and permeability are unknowns. Initial core temperature is varied over several hundred oC, and permeability is varied over 1-100 millidarcies. In our simulations, typically, a flow field develops characterized by sinking flow at the equator and rising plumes at the poles. A broad thickening of ice in the equatorial region occurs, so much so that flow is gradually restricted to the polar regions, with the south pole flow stronger than at the northern pole. A feedback develops; cooler, sinking flow at the equator results in thickening of the ice there which in turn tends to isolate flow to the deeper ocean plus core region at the poles. The rate at which this pattern develops depends on the presence or absence of TDH. Except at the surface, a nearly cylindrical region from north to south through the model remains fluid. The presence of salt and/or NH3 allows liquid conditions and flow even as the ocean temperature falls well below 0 oC. At higher initial core temperatures, boiling occurs deep in the core because of the low overburden pressure. An approximately 70 km thick difference in ice thickness can develop between equator and poles. However, due to the low gravity of Enceladus, this would give rise to a buoyant pressure difference of only about 5 bars, which is less than shear strength measurements in ice. The core slowly cools, and eventually the ocean may freeze completely without TDH, but that can take on the order of several hundred million years or more. If episodes of strong TDH occurred on that time scale or shorter, a polar ocean might then persist indefinitely.

Assessment of the hygienic performances of hamburger patty production processes.

PubMed

Gill, C O; Rahn, K; Sloan, K; McMullen, L M

1997-05-20

The hygienic conditions of the hamburger patties collected from three patty manufacturing plants and six retail outlets were examined. At each manufacturing plant a sample from newly formed, chilled patties and one from frozen patties were collected from each of 25 batches of patties selected at random. At three, two or one retail outlet, respectively, 25 samples from frozen, chilled or both frozen and chilled patties were collected at random. Each sample consisted of 30 g of meat obtained from five or six patties. Total aerobic, coliform and Escherichia coli counts per gram were enumerated for each sample. The mean log (x) and standard deviation (s) were calculated for the log10 values for each set of 25 counts, on the assumption that the distribution of counts approximated the log normal. A value for the log10 of the arithmetic mean (log A) was calculated for each set from the values of x and s. A chi2 statistic was calculated for each set as a test of the assumption of the log normal distribution. The chi2 statistic was calculable for 32 of the 39 sets. Four of the sets gave chi2 values indicative of gross deviation from log normality. On inspection of those sets, distributions obviously differing from the log normal were apparent in two. Log A values for total, coliform and E. coli counts for chilled patties from manufacturing plants ranged from 4.4 to 5.1, 1.7 to 2.3 and 0.9 to 1.5, respectively. Log A values for frozen patties from manufacturing plants were between < 0.1 and 0.5 log10 units less than the equivalent values for chilled patties. Log A values for total, coliform and E. coli counts for frozen patties on retail sale ranged from 3.8 to 8.5, < 0.5 to 3.6 and < 0 to 1.9, respectively. The equivalent ranges for chilled patties on retail sale were 4.8 to 8.5, 1.8 to 3.7 and 1.4 to 2.7, respectively. The findings indicate that the general hygienic condition of hamburgers patties could be improved by their being manufactured from only manufacturing beef of superior hygienic quality, and by the better management of chilled patties at retail outlets.

A spatially explicit decision support model for restoration of forest bird habitat

USGS Publications Warehouse

Twedt, D.J.; Uihlein, W.B.; Elliott, A.B.

2006-01-01

The historical area of bottomland hardwood forest in the Mississippi Alluvial Valley has been reduced by >75%. Agricultural production was the primary motivator for deforestation; hence, clearing deliberately targeted higher and drier sites. Remaining forests are highly fragmented and hydrologically altered, with larger forest fragments subject to greater inundation, which has negatively affected many forest bird populations. We developed a spatially explicit decision support model, based on a Partners in Flight plan for forest bird conservation, that prioritizes forest restoration to reduce forest fragmentation and increase the area of forest core (interior forest >1 km from 'hostile' edge). Our primary objective was to increase the number of forest patches that harbor >2000 ha of forest core, but we also sought to increase the number and area of forest cores >5000 ha. Concurrently, we targeted restoration within local (320 km2) landscapes to achieve >60% forest cover. Finally, we emphasized restoration of higher-elevation bottomland hardwood forests in areas where restoration would not increase forest fragmentation. Reforestation of 10% of restorable land in the Mississippi Alluvial Valley (approximately 880,000 ha) targeted at priorities established by this decision support model resulted in approximately 824,000 ha of new forest core. This is more than 32 times the amount of core forest added through reforestation of randomly located fields (approximately 25,000 ha). The total area of forest core (1.6 million ha) that resulted from targeted restoration exceeded habitat objectives identified in the Partners in Flight Bird Conservation Plan and approached the area of forest core present in the 1950s.

Mechanical Alteration And Contamination Issues In Automated Subsurface Sample Acquisition And Handling

NASA Astrophysics Data System (ADS)

Glass, B. J.; Cannon, H.; Bonaccorsi, R.; Zacny, K.

2006-12-01

The Drilling Automation for Mars Exploration (DAME) project's purpose is to develop and field-test drilling automation and robotics technologies for projected use in missions in the 2011-15 period. DAME includes control of the drilling hardware, and state estimation of both the hardware and the lithography being drilled and the state of the hole. A sister drill was constructed for the Mars Analog Río Tinto Experiment (MARTE) project and demonstrated automated core handling and string changeout in 2005 drilling tests at Rio Tinto, Spain. DAME focused instead on the problem of drill control while actively drilling while not getting stuck. Together, the DAME and MARTE projects demonstrate a fully automated robotic drilling capability, including hands-off drilling, adjustment to different strata and downhole conditions, recovery from drilling faults (binding, choking, etc.), drill string changeouts, core acquisition and removal, and sample handling and conveyance to in-situ instruments. The 2006 top-level goal of DAME drilling in-situ tests was to verify and demonstrate a capability for hands-off automated drilling, at an Arctic Mars-analog site. There were three sets of 2006 test goals, all of which were exceeded during the July 2006 field season. The first was to demonstrate the recognition, while drilling, of at least three of the six known major fault modes for the DAME planetary-prototype drill, and to employ the correct recovery or safing procedure in response. The second set of 2006 goals was to operate for three or more hours autonomously, hands-off. And the third 2006 goal was to exceed 3m depth into the frozen breccia and permafrost with the DAME drill (it had not gone further than 2.2m previously). Five of six faults were detected and corrected, there were 43 hours of hands-off drilling (including a 4 hour sequence with no human presence nearby), and 3.2m was the total depth. And ground truth drilling used small commercial drilling equipment in parallel in order to obtain cores and ice profiles at the drilling site. In the course of DAME drilling automation testing, the drilling-induced temperature gradients and their effects on encountered subsurface permafrost and ice layers were observed while drilling in frozen impact breccia at Haughton Crater. In repeated tests of robotic core removal processing and handling in the MARTE project, including field tests, cross-contamination issues arose between successive cores and samples, and procedures and metrics were developed for minimizing the cross-contamination. The MARTE core processing cross-contamination aspects were tested by analyzing a set of pristine samples (those stratigraphically known) vs. cuttings (loose clays) or artifacts from the robotic drilling (indurated clay layers). MARTE ground truth drilling, in parallel with the automated tests, provided control information on the discontinuity/continuity of the stratigraphic record (i.e., texture, color and structure of loose and consolidated materials).

Application of ground-penetrating radar imagery for three-dimensional visualisation of near-surface structures in ice-rich permafrost, Barrow, Alaska

USGS Publications Warehouse

Munroe, Jeffrey S.; Doolittle, James A.; Kanevskiy, Mikhail; Hinkel, Kenneth M.; Nelson, Frederick E.; Jones, Benjamin M.; Shur, Yuri; Kimble, John M.

2007-01-01

Three-dimensional ground-penetrating radar (3D GPR) was used to investigate the subsurface structure of ice-wedge polygons and other features of the frozen active layer and near-surface permafrost near Barrow, Alaska. Surveys were conducted at three sites located on landscapes of different geomorphic age. At each site, sediment cores were collected and characterised to aid interpretation of GPR data. At two sites, 3D GPR was able to delineate subsurface ice-wedge networks with high fidelity. Three-dimensional GPR data also revealed a fundamental difference in ice-wedge morphology between these two sites that is consistent with differences in landscape age. At a third site, the combination of two-dimensional and 3D GPR revealed the location of an active frost boil with ataxitic cryostructure. When supplemented by analysis of soil cores, 3D GPR offers considerable potential for imaging, interpreting and 3D mapping of near-surface soil and ice structures in permafrost environments.

Comet sample acquisition for ROSETTA lander mission

NASA Astrophysics Data System (ADS)

Marchesi, M.; Campaci, R.; Magnani, P.; Mugnuolo, R.; Nista, A.; Olivier, A.; Re, E.

2001-09-01

ROSETTA/Lander is being developed with a combined effort of European countries, coordinated by German institutes. The commitment for such a challenging probe will provide a unique opportunity for in-situ analysis of a comet nucleus. The payload for coring, sampling and investigations of comet materials is called SD2 (Sampling Drilling and Distribution). The paper presents the drill/sampler tool and the sample transfer trough modeling, design and testing phases. Expected drilling parameters are then compared with experimental data; limited torque consumption and axial thrust on the tool constraint the operation and determine the success of tests. Qualification campaign involved the structural part and related vibration test, the auger/bit parts and drilling test, and the coring mechanism with related sampling test. Mechanical check of specimen volume is also reported, with emphasis on the measurement procedure and on the mechanical unit. The drill tool and all parts of the transfer chain were tested in the hypothetical comet environment, charcterized by frozen material at extreme low temperature and high vacuum (-160°C, 10-3 Pa).

A Search for O2 in CO-Depleted Molecular Cloud Cores With Herschel

NASA Technical Reports Server (NTRS)

Wirstroem, Eva S.; Charnley, Steven B.; Cordiner, Martin; Ceccarelli, Cecilia

2016-01-01

The general lack of molecular oxygen in molecular clouds is an outstanding problem in astrochemistry. Extensive searches with the Submillimeter Astronomical Satellite, Odin, and Herschel have only produced two detections; upper limits to the O2 abundance in the remaining sources observed are about 1000 times lower than predicted by chemical models. Previous atomic oxygen observations and inferences from observations of other molecules indicated that high abundances of O atoms might be present in dense cores exhibiting large amounts of CO depletion. Theoretical arguments concerning the oxygen gas-grain interaction in cold dense cores suggested that, if O atoms could survive in the gas after most of the rest of the heavy molecular material has frozen out onto dust, then O2 could be formed efficiently in the gas. Using Herschel HIFI, we searched a small sample of four depletion cores-L1544, L694-2, L429, and Oph D-for emission in the low excitation O2 N(sub J)?=?3(sub 3)-1(sub 2) line at 487.249 GHz. Molecular oxygen was not detected and we derive upper limits to its abundance in the range of N(O2)/N (H2) approx. = (0.6-1.6) x10(exp -7). We discuss the absence of O2 in the light of recent laboratory and observational studies.

A Limited Survey of Heavy Metal Concentrations in Fresh and Frozen Cuttlefish Ink and Mantle Used As Food.

PubMed

Conficoni, Daniele; Alberghini, Leonardo; Bissacco, Elisa; Contiero, Barbara; Giaccone, Valerio

2018-02-01

Cuttlefish ink is consumed as a delicacy worldwide. The current study is the first assessment of heavy metal concentrations in cuttlefish ink versus mantle under different storage methods. A total of 212 samples (64 of fresh mantle, 42 of frozen mantle, 64 of fresh ink, and 42 of frozen ink) were analyzed for the detection of the following heavy metals: arsenic (As), chromium (Cr), iron (Fe), lead (Pb), mercury (Hg), and cadmium (Cd). The median As concentrations were 12.9 mg/kg for fresh mantle, 8.63 mg/kg for frozen mantle, 10.8 mg/kg for frozen ink, and 0.41 mg/kg for fresh ink. The median Cr concentrations were 0.06 mg/kg for fresh mantle and frozen ink, 0.03 mg/kg for frozen mantle, and below the limit of quantification (LOQ) for fresh ink. The median Fe concentrations were 4.08 mg/kg for frozen ink, 1.51 mg/kg for fresh mantle, 0.73 mg/kg for frozen mantle, and below the LOQ for fresh ink. The median Pb concentrations of almost all samples were below the LOQ; only two frozen ink, one fresh ink, one frozen mantle, and one fresh mantle sample exceeded the limit stipulated by the European Union. The Hg concentrations were statistically similar among the four categories of samples; the median Hg concentrations were below the LOQ, and the maximum concentrations were found in frozen ink, at 1.62 mg/kg. The median Cd concentrations were 0.69 mg/kg for frozen ink and 0.11 mg/kg for frozen mantle, fresh mantle and fresh ink concentrations were below the LOQ, and in 11.3% of the tested samples, Cd concentrations were higher than the European Union limit. The probability of samples having a Cd concentration above the legal limit was 35.75 times higher in frozen than in fresh products. Fresh ink had significantly lower concentrations of As, Cr, Fe, and Cd, but the concentrations of Hg and Pb were not significantly different from those of other products. Frozen ink had significantly higher concentrations of Cd, Cr, and Fe, but concentrations of As were lower than those in fresh mantle, pointing out a possible role for the freezing process and for different fishing zones as risk factors for heavy metal contamination.

Diagenetic changes of lignin compounds in a more than 0.6 million-year-old lacustrine sediment (Lake Biwa, Japan)

NASA Astrophysics Data System (ADS)

Ishiwatari, Ryoshi; Uzaki, Minoru

1987-02-01

A vertical profile of lignin in the upper 700 m layer of a 1400 m sediment core of Lake Biwa, an oligotrophic freshwater lake in Japan, was determined using a CuO oxidative degradation method. The results indicated that lignin is found throughout the core, demonstrating lignin to be very stable for over 0.6 million years. Moreover, the upper 250 m (approximately 0.6 million years old) segment of the sediment core was investigated to determine the apparent long term degradation rate of lignin. A downward lignin concentration decrease is observed over the upper 250 m of the core which corresponds to a calculated half life of at least approximately 40 × 10 4 years, assuming that lignin decrease is due to its in situ degradation (diagenesis).

The unstaggered extension to GFDL's FV3 dynamical core on the cubed-sphere

NASA Astrophysics Data System (ADS)

Chen, X.; Lin, S. J.; Harris, L.

2017-12-01

Finite-volume schemes have become popular for atmospheric transport since they provide intrinsic mass conservation to constituent species. Many CFD codes use unstaggered discretizations for finite volume methods with an approximate Riemann solver. However, this approach is inefficient for geophysical flows due to the complexity of the Riemann solver. We introduce a Low Mach number Approximate Riemann Solver (LMARS) simplified using assumptions appropriate for atmospheric flows: the wind speed is much slower than the sound speed, weak discontinuities, and locally uniform sound wave velocity. LMARS makes possible a Riemann-solver-based dynamical core comparable in computational efficiency to many current dynamical cores. We will present a 3D finite-volume dynamical core using LMARS in a cubed-sphere geometry with a vertically Lagrangian discretization. Results from standard idealized test cases will be discussed.

Fusion reaction cross-sections using the Wong model within Skyrme energy density based semiclassical extended Thomas Fermi approach

NASA Astrophysics Data System (ADS)

Kumar, Raj; Sharma, Manoj K.; Gupta, Raj K.

2011-11-01

First, the nuclear proximity potential, obtained by using the semiclassical extended Thomas Fermi (ETF) approach in Skyrme energy density formalism (SEDF), is shown to give more realistic barriers in frozen density approximation, as compared to the sudden approximation. Then, taking advantage of the fact that, in ETF method, different Skyrme forces give different barriers (height, position and curvature), we use the ℓ-summed extended-Wong model of Gupta and collaborators (2009) [1] under frozen densities approximation for calculating the cross-sections, where the Skyrme force is chosen with proper barrier characteristics, not-requiring additional "barrier modification" effects (lowering or narrowing, etc.), for a best fit to data at sub-barrier energies. The method is applied to capture cross-section data from 48Ca + 238U, 244Pu, and 248Cm reactions and to fusion-evaporation cross-sections from 58Ni + 58Ni, 64Ni + 64Ni, and 64Ni + 100Mo reactions, with effects of deformations and orientations of nuclei included, wherever required. Interestingly, whereas the capture cross-sections in Ca-induced reactions could be fitted to any force, such as SIII, SV and GSkI, by allowing a small change of couple of units in deduced ℓ-values at below-barrier energies, the near-barrier data point of 48Ca + 248Cm reaction could not be fitted to ℓ-values deduced for below-barrier energies, calling for a check of data. On the other hand, the fusion-evaporation cross-sections in Ni-induced reactions at sub-barrier energies required different Skyrme forces, representing "modifications of the barrier", for the best fit to data at all incident center-of-mass energies E's, displaying a kind of fusion hindrance at sub-barrier energies. This barrier modification effect is taken into care here by using different Skyrme forces for reactions belonging to different regions of the periodic table. Note that more than one Skyrme force (with identical barrier characteristics) could equally well fit the same data.

Dissociation of MgSiO3 in the cores of gas giants and terrestrial exoplanets.

PubMed

Umemoto, Koichiro; Wentzcovitch, Renata M; Allen, Philip B

2006-02-17

CaIrO3-type MgSiO3 is the planet-forming silicate stable at pressures and temperatures beyond those of Earth's core-mantle boundary. First-principles quasiharmonic free-energy computations show that this mineral should dissociate into CsCl-type MgO cotunnite-type SiO2 at pressures and temperatures expected to occur in the cores of the gas giants + and in terrestrial exoplanets. At approximately 10 megabars and approximately 10,000 kelvin, cotunnite-type SiO2 should have thermally activated electron carriers and thus electrical conductivity close to metallic values. Electrons will give a large contribution to thermal conductivity, and electronic damping will suppress radiative heat transport.

CFD analysis of a twin scroll radial turbine

NASA Astrophysics Data System (ADS)

Fürst, Jiří; Žák, Zdenĕk

2018-06-01

The contribution deals with the application of coupled implicit solver for compressible flows to CFD analysis of a twin scroll radial turbine. The solver is based on the finite volume method, convective terms are approximated using AUSM+up scheme, viscous terms use central approximation and the time evolution is achieved with lower-upper symmetric Gauss-Seidel (LU-SGS) method. The solver allows steady simulation with the so called frozen rotor approach as well as the fully unsteady solution. Both approaches are at first validated for the case of ERCOFTAC pump [1]. Then the CFD analysis of the flow through a twin scroll radial turbine and the predictions of the efficiency and turbine power is performed and the results are compared to experimental data obtained in the framework of Josef Božek - Competence Centre for Automotive Industry.

Frozen Paradise

NASA Image and Video Library

2015-03-02

Named after a Japanese paradise, the Senkyo region of Titan (the dark area below and to the right of center) is a bit less welcoming than its namesake. With a very inhospitable average temperature of approximately 290 degrees below zero Fahrenheit (-180 degrees Celsius), water on Titan (3,200 miles or 5,150 kilometers across) freezes hard enough to be essentially considered rock. This view looks toward the Saturn-facing side of Titan. North on Titan is up and rotated 33 degrees to the right. The image was taken with the Cassini spacecraft narrow-angle camera on Jan. 8, 2015 using a near-infrared filter which is centered at 938 nanometers. The view was acquired at a distance of approximately 1.2 million miles (1.9 million kilometers) from Titan. Image scale is 7 miles (11 kilometers) per pixel. http://photojournal.jpl.nasa.gov/catalog/pia18309

Core-core and core-valence correlation

NASA Technical Reports Server (NTRS)

Bauschlicher, Charles W., Jr.; Langhoff, Stephen R.; Taylor, Peter R.

1988-01-01

The effect of 1s core correlation on properties and energy separations are analyzed using full configuration-interaction (FCI) calculations. The Be1S - 1P, the C 3P - 5S,m and CH(+) 1Sigma(+) - 1Pi separations, and CH(+) spectroscopic constants, dipole moment, and 1Sigma(+) - 1Pi transition dipole moment have been studied. The results of the FCI calculations are compared to those obtained using approximate methods.

Calculation of nuclear spin-spin coupling constants using frozen density embedding

DOE Office of Scientific and Technical Information (OSTI.GOV)

Götz, Andreas W., E-mail: agoetz@sdsc.edu; Autschbach, Jochen; Visscher, Lucas, E-mail: visscher@chem.vu.nl

2014-03-14

We present a method for a subsystem-based calculation of indirect nuclear spin-spin coupling tensors within the framework of current-spin-density-functional theory. Our approach is based on the frozen-density embedding scheme within density-functional theory and extends a previously reported subsystem-based approach for the calculation of nuclear magnetic resonance shielding tensors to magnetic fields which couple not only to orbital but also spin degrees of freedom. This leads to a formulation in which the electron density, the induced paramagnetic current, and the induced spin-magnetization density are calculated separately for the individual subsystems. This is particularly useful for the inclusion of environmental effects inmore » the calculation of nuclear spin-spin coupling constants. Neglecting the induced paramagnetic current and spin-magnetization density in the environment due to the magnetic moments of the coupled nuclei leads to a very efficient method in which the computationally expensive response calculation has to be performed only for the subsystem of interest. We show that this approach leads to very good results for the calculation of solvent-induced shifts of nuclear spin-spin coupling constants in hydrogen-bonded systems. Also for systems with stronger interactions, frozen-density embedding performs remarkably well, given the approximate nature of currently available functionals for the non-additive kinetic energy. As an example we show results for methylmercury halides which exhibit an exceptionally large shift of the one-bond coupling constants between {sup 199}Hg and {sup 13}C upon coordination of dimethylsulfoxide solvent molecules.« less

Frozen O 2 layer revealed by neutron reflectometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Steffen, A.; Glavic, A.; Holderer, O.

2016-05-27

We investigated a 63 thick film originating from frozen air on a solid substrate via neutron reflectometry. Furthermore, the experiment shows that neutron reflectometry allows performing chemical surface analysis by quantifying the composition of this frozen layer and identifies the film to be frozen oxygen.

40 CFR 407.40 - Applicability; description of the frozen potato products subcategory.

Code of Federal Regulations, 2010 CFR

2010-07-01

... frozen potato products subcategory. 407.40 Section 407.40 Protection of Environment ENVIRONMENTAL... PROCESSING POINT SOURCE CATEGORY Frozen Potato Products Subcategory § 407.40 Applicability; description of the frozen potato products subcategory. The provisions of this subpart are applicable to discharges...

40 CFR 407.40 - Applicability; description of the frozen potato products subcategory.

Code of Federal Regulations, 2013 CFR

2013-07-01

... frozen potato products subcategory. 407.40 Section 407.40 Protection of Environment ENVIRONMENTAL... PROCESSING POINT SOURCE CATEGORY Frozen Potato Products Subcategory § 407.40 Applicability; description of the frozen potato products subcategory. The provisions of this subpart are applicable to discharges...

40 CFR 407.40 - Applicability; description of the frozen potato products subcategory.

Code of Federal Regulations, 2014 CFR

2014-07-01

... frozen potato products subcategory. 407.40 Section 407.40 Protection of Environment ENVIRONMENTAL... PROCESSING POINT SOURCE CATEGORY Frozen Potato Products Subcategory § 407.40 Applicability; description of the frozen potato products subcategory. The provisions of this subpart are applicable to discharges...

40 CFR 407.40 - Applicability; description of the frozen potato products subcategory.

Code of Federal Regulations, 2012 CFR

2012-07-01

... frozen potato products subcategory. 407.40 Section 407.40 Protection of Environment ENVIRONMENTAL... PROCESSING POINT SOURCE CATEGORY Frozen Potato Products Subcategory § 407.40 Applicability; description of the frozen potato products subcategory. The provisions of this subpart are applicable to discharges...

40 CFR 407.40 - Applicability; description of the frozen potato products subcategory.

Code of Federal Regulations, 2011 CFR

2011-07-01

... frozen potato products subcategory. 407.40 Section 407.40 Protection of Environment ENVIRONMENTAL... PROCESSING POINT SOURCE CATEGORY Frozen Potato Products Subcategory § 407.40 Applicability; description of the frozen potato products subcategory. The provisions of this subpart are applicable to discharges...

[Adaptability of sweet corn ears to a frozen process].

PubMed

Ramírez Matheus, Alejandra O; Martínez, Norelkys Maribel; de Bertorelli, Ligia O; De Venanzi, Frank

2004-12-01

The effects of frozen condition on the quality of three sweet corn ears (2038, 2010, 2004) and the pattern (Bonanza), were evaluated. Biometrics characteristics like ear size, ear diameter, row and kernel deep were measured as well as chemical and physical measurement in fresh and frozen states. The corn ears were frozen at -95 degrees C by 7 minutes. The yield and stability of the frozen ears were evaluated at 45 and 90 days of frozen storage (-18 degrees C). The average commercial yield as frozen corn ear for all the hybrids was 54.2%. The industry has a similar value range of 48% to 54%. The ear size average was 21.57 cm, row number was 15, ear diameter 45.54 mm and the kernel corn deep was 8.57 mm. All these measurements were found not different from commercial values found for the industry. All corn samples evaluated showed good stability despites the frozen processing and storage. Hybrid 2038 ranked higher in quality.

Pair natural orbital and canonical coupled cluster reaction enthalpies involving light to heavy alkali and alkaline earth metals: the importance of sub-valence correlation.

PubMed

Minenkov, Yury; Bistoni, Giovanni; Riplinger, Christoph; Auer, Alexander A; Neese, Frank; Cavallo, Luigi

2017-04-05

In this work, we tested canonical and domain based pair natural orbital coupled cluster methods (CCSD(T) and DLPNO-CCSD(T), respectively) for a set of 32 ligand exchange and association/dissociation reaction enthalpies involving ionic complexes of Li, Be, Na, Mg, Ca, Sr, Ba and Pb(ii). Two strategies were investigated: in the former, only valence electrons were included in the correlation treatment, giving rise to the computationally very efficient FC (frozen core) approach; in the latter, all non-ECP electrons were included in the correlation treatment, giving rise to the AE (all electron) approach. Apart from reactions involving Li and Be, the FC approach resulted in non-homogeneous performance. The FC approach leads to very small errors (<2 kcal mol -1 ) for some reactions of Na, Mg, Ca, Sr, Ba and Pb, while for a few reactions of Ca and Ba deviations up to 40 kcal mol -1 have been obtained. Large errors are both due to artificial mixing of the core (sub-valence) orbitals of metals and the valence orbitals of oxygen and halogens in the molecular orbitals treated as core, and due to neglecting core-core and core-valence correlation effects. These large errors are reduced to a few kcal mol -1 if the AE approach is used or the sub-valence orbitals of metals are included in the correlation treatment. On the technical side, the CCSD(T) and DLPNO-CCSD(T) results differ by a fraction of kcal mol -1 , indicating the latter method as the perfect choice when the CPU efficiency is essential. For completely black-box applications, as requested in catalysis or thermochemical calculations, we recommend the DLPNO-CCSD(T) method with all electrons that are not covered by effective core potentials included in the correlation treatment and correlation-consistent polarized core valence basis sets of cc-pwCVQZ(-PP) quality.

Outcomes Related to the Use of Frozen Plasma or Pooled Solvent/Detergent-Treated Plasma in Critically Ill Children.

PubMed

Camazine, Maraya N; Karam, Oliver; Colvin, Ryan; Leteurtre, Stephane; Demaret, Pierre; Tucci, Marisa; Muszynski, Jennifer A; Stanworth, Simon; Spinella, Philip C

2017-05-01

To determine if the use of fresh frozen plasma/frozen plasma 24 hours compared to solvent detergent plasma is associated with international normalized ratio reduction or ICU mortality in critically ill children. This is an a priori secondary analysis of a prospective, observational study. Study groups were defined as those transfused with either fresh frozen plasma/frozen plasma 24 hours or solvent detergent plasma. Outcomes were international normalized ratio reduction and ICU mortality. Multivariable logistic regression was used to determine independent associations. One hundred one PICUs in 21 countries. All critically ill children admitted to a participating unit were included if they received at least one plasma unit during six predefined 1-week (Monday to Friday) periods. All children were exclusively transfused with either fresh frozen plasma/frozen plasma 24 hours or solvent detergent plasma. None. There were 443 patients enrolled in the study. Twenty-four patients (5%) were excluded because no plasma type was recorded; the remaining 419 patients were analyzed. Fresh frozen plasma/frozen plasma 24 hours group included 357 patients, and the solvent detergent plasma group included 62 patients. The median (interquartile range) age and weight were 1 year (0.2-6.4) and 9.4 kg (4.0-21.1), respectively. There was no difference in reason for admission, severity of illness score, pretransfusion international normalized ratio, or lactate values; however, there was a difference in primary indication for plasma transfusion (p < 0.001). There was no difference in median (interquartile range) international normalized ratio reduction, between fresh frozen plasma/frozen plasma 24 hours and solvent detergent plasma study groups, -0.2 (-0.4 to 0) and -0.2 (-0.3 to 0), respectively (p = 0.80). ICU mortality was lower in the solvent detergent plasma versus fresh frozen plasma/frozen plasma 24 hours groups, 14.5% versus 29.1%%, respectively (p = 0.02). Upon adjusted analysis, solvent detergent plasma transfusion was independently associated with reduced ICU mortality (odds ratio, 0.40; 95% CI, 0.16-0.99; p = 0.05). Solvent detergent plasma use in critically ill children may be associated with improved survival. This hypothesis-generating data support a randomized controlled trial comparing solvent detergent plasma to fresh frozen plasma/frozen plasma 24 hours.

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2010 CFR

2010-10-01

... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Frozen processed foods...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

Effect of freezing on the rheological, chemical and colour properties of Serpa cheese.

PubMed

Alvarenga, Nuno; Canada, João; Sousa, Isabel

2011-02-01

The effect of freezing on the properties of a raw ewes'-milk semi-soft cheese (Serpa cheese) was studied using small amplitude oscillatory (SAOS) and texture measurements, colour and chemical parameters. The freezing was introduced at three different stages of the ripening process (28, 35 and 42 days), and the cheeses were maintained frozen for 12 months. Cheeses were submitted to a slow or fast freezing method, and to different storage temperatures: -10 and -20°C (three replicates for each set conditions). Chemical data showed that only the proteolysis indicators exhibited differences between frozen and non-frozen samples; frozen samples showed higher values of NPN than the non-frozen samples, indicating that the freezing process did not prevent the secondary proteolysis of cheese. Frozen samples showed a significantly (P<0·05) stronger structure than the non-frozen, as indicated by hardness. However, the differences between the frozen and non-frozen samples were not significantly for storage modulus (G' 1Hz) and loss tangent (tan δ 1Hz) (P>0·05). Freezing affected mainly colour parameters: frozen samples were more luminous, and more yellow-green. The results allowed us to conclude that the damages caused by freezing to cheese properties could be minimized if this type of storage is introduced at the end of ripening (42 d) using a freezing temperature of -20°C.

Characteristics and possible formation mechanisms of severe storms in the outer rainbands of Typhoon Mujiga (1522)

NASA Astrophysics Data System (ADS)

Wang, Bingyun; Wei, Ming; Hua, Wei; Zhang, Yongli; Wen, Xiaohang; Zheng, Jiafeng; Li, Nan; Li, Han; Wu, Yu; Zhu, Jie; Zhang, Mingjun

2017-06-01

To better understand how severe storms form and evolve in the outer rainbands of typhoons, in this study, we investigate the evolutionary characteristics and possible formation mechanisms for severe storms in the rainbands of Typhoon Mujigae, which occurred during 2-5 October 2015, based on the NCEP-NCAR reanalysis data, conventional observations, and Doppler radar data. For the rainbands far from the inner core (eye and eyewall) of Mujigae (distance of approximately 70-800 km), wind speed first increased with the radius expanding from the inner core, and then decreased as the radius continued to expand. The Rankine Vortex Model was used to explore such variations in wind speed. The areas of strong stormy rainbands were mainly located in the northeast quadrant of Mujigae, and overlapped with the areas of high winds within approximately 300-550 km away from the inner core, where the strong winds were conducive to the development of strong storms. A severe convective cell in the rainbands developed into waterspout at approximately 500 km to the northeast of the inner core, when Mujigae was strengthening before it made landfall. Two severe convective cells in the rainbands developed into two tornadoes at approximately 350 km to the northeast of the inner core after Mujigae made landfall. The radar echo bands enhanced to 60 dBZ when mesocyclones occurred in the rainbands and induced tornadoes. The radar echoes gradually weakened after the mesocyclones weakened. The tops of parent clouds of the mesocyclones elevated at first, and then suddenly dropped about 20 min before the tornadoes appeared. Thereby, the cloud top variation has the potential to be used as an early warning of tornado occurrence.

Double-core evolution. 5: Three-dimensional effects in the merger of a red giant with a dwarf companion

NASA Technical Reports Server (NTRS)

Terman, James L.; Taam, Ronald E.; Hernquist, Lars

1994-01-01

The evolution of the common envelope phase of a binary system consisting of a 4.67 solar mass red giant and a 0.94 solar mass dwarf is studied using smoothed particle hydrodynamics. We demonstrate that the three-dimensional effects associated with the gravitational tidal torques lead to a rapid decay of the orbit on timescales approximately less than 1 yr. The relative orbit of the two cores in the common envelope is initally eccentric and tends to circularize as the orbital separation of the two cores decreases. The angular momentum lost from the orbital motion is distributed throughout the common envelope, and the double core does not evolve to a state of co-rotation for the evolutionary time followed. The energy dissipated from the relative orbit and deposited in the common envelope results in the ejection of approximately 13% of the mass of the envelope. The mass is ejected in all directions, but there is a preference for mass ejection in the orbital plane of the binary system. For example, approximately 80% of the ejected mass lies within 30 deg of the binary orbital plane. Because gravitational forces are long range, most of the energy and angular momentum is imparted to a small fraction of the common envelope resulting in an efficiency of the mass ejection process of approximately 15%. The core of the red giant executes significant displacement with respect to the center of mass of the system and contributes nearly equally to the total energy dissipation rate during the latter phases of the evolution. The degree of departure from synchronism of the initial binary system can be an important property of the system which can affect the outcome of the common envelope phase.

Incidence and prognostic factors for postoperative frozen shoulder after shoulder surgery: a prospective cohort study.

PubMed

Koorevaar, Rinco C T; Van't Riet, Esther; Ipskamp, Marcel; Bulstra, Sjoerd K

2017-03-01

Frozen shoulder is a potential complication after shoulder surgery. It is a clinical condition that is often associated with marked disability and can have a profound effect on the patient's quality of life. The incidence, etiology, pathology and prognostic factors of postoperative frozen shoulder after shoulder surgery are not known. The purpose of this explorative study was to determine the incidence of postoperative frozen shoulder after various operative shoulder procedures. A second aim was to identify prognostic factors for postoperative frozen shoulder after shoulder surgery. 505 consecutive patients undergoing elective shoulder surgery were included in this prospective cohort study. Follow-up was 6 months after surgery. A prediction model was developed to identify prognostic factors for postoperative frozen shoulder after shoulder surgery using the TRIPOD guidelines. We nominated five potential predictors: gender, diabetes mellitus, type of physiotherapy, arthroscopic surgery and DASH score. Frozen shoulder was identified in 11% of the patients after shoulder surgery and was more common in females (15%) than in males (8%). Frozen shoulder was encountered after all types of operative procedures. A prediction model based on four variables (diabetes mellitus, specialized shoulder physiotherapy, arthroscopic surgery and DASH score) discriminated reasonably well with an AUC of 0.712. Postoperative frozen shoulder is a serious complication after shoulder surgery, with an incidence of 11%. Four prognostic factors were identified for postoperative frozen shoulder: diabetes mellitus, arthroscopic surgery, specialized shoulder physiotherapy and DASH score. The combination of these four variables provided a prediction rule for postoperative frozen shoulder with reasonable fit. Level II, prospective cohort study.

Arrangement of photosystem II and ATP synthase in chloroplast membranes of spinach and pea.

PubMed

Daum, Bertram; Nicastro, Daniela; Austin, Jotham; McIntosh, J Richard; Kühlbrandt, Werner

2010-04-01

We used cryoelectron tomography to reveal the arrangements of photosystem II (PSII) and ATP synthase in vitreous sections of intact chloroplasts and plunge-frozen suspensions of isolated thylakoid membranes. We found that stroma and grana thylakoids are connected at the grana margins by staggered lamellar membrane protrusions. The stacking repeat of grana membranes in frozen-hydrated chloroplasts is 15.7 nm, with a 4.5-nm lumenal space and a 3.2-nm distance between the flat stromal surfaces. The chloroplast ATP synthase is confined to minimally curved regions at the grana end membranes and stroma lamellae, where it covers 20% of the surface area. In total, 85% of the ATP synthases are monomers and the remainder form random assemblies of two or more copies. Supercomplexes of PSII and light-harvesting complex II (LHCII) occasionally form ordered arrays in appressed grana thylakoids, whereas this order is lost in destacked membranes. In the ordered arrays, each membrane on either side of the stromal gap contains a two-dimensional crystal of supercomplexes, with the two lattices arranged such that PSII cores, LHCII trimers, and minor LHCs each face a complex of the same kind in the opposite membrane. Grana formation is likely to result from electrostatic interactions between these complexes across the stromal gap.

76 FR 64001 - United States Standards for Grades of Frozen Okra

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-17

... to undertaking research and other work associated with revising the grade standards, AMS sought...-327] United States Standards for Grades of Frozen Okra AGENCY: Agricultural Marketing Service, USDA... (USDA) has revised the United States Standards for Grades of Frozen Okra. The grade standards for frozen...

Synthesis of SiO2-coated ZnMnFe2O4 nanospheres with improved magnetic properties.

PubMed

Wang, Jun; Zhang, Kai; Zhu, Yuejin

2005-05-01

A core-shell structured composite, SiO2 coated ZnMnFe2O4 spinel ferrite nanoparticles (average diameter of approximately 80 nm), was prepared by hydrolysis of tetraethyl orthosilicate (TEOS) in the presence of ZnMnFe2O4 nanoparticles (average diameter of approximately 10 nm) synthesized by a hydrothermal method. The obtained samples were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FESEM). The magnetic measurements were carried out on a vibrating sample magnetometer (VSM), and the measurement results indicate that the core-shell samples possess better magnetic properties at room temperature, compared with paramagnetic colloids with a magnetic core by a coprecipitation method. These core-shell nanospherical particles with self-assembly under additional magnetic fields could have potential application in biomedical systems.

Performance of the Falling Snow Retrieval Algorithms for the Global Precipitation Measurement (GPM) Mission

NASA Technical Reports Server (NTRS)

Skofronick-Jackson, Gail; Munchak, Stephen J.; Ringerud, Sarah

2016-01-01

Retrievals of falling snow from space represent an important data set for understanding the Earth's atmospheric, hydrological, and energy cycles, especially during climate change. Estimates of falling snow must be captured to obtain the true global precipitation water cycle, snowfall accumulations are required for hydrological studies, and without knowledge of the frozen particles in clouds one cannot adequately understand the energy and radiation budgets. While satellite-based remote sensing provides global coverage of falling snow events, the science is relatively new and retrievals are still undergoing development with challenges remaining). This work reports on the development and testing of retrieval algorithms for the Global Precipitation Measurement (GPM) mission Core Satellite, launched February 2014.

Principles of quasi-equivalence and Euclidean geometry govern the assembly of cubic and dodecahedral cores of pyruvate dehydrogenase complexes.

PubMed

Izard, T; Aevarsson, A; Allen, M D; Westphal, A H; Perham, R N; de Kok, A; Hol, W G

1999-02-16

The pyruvate dehydrogenase multienzyme complex (Mr of 5-10 million) is assembled around a structural core formed of multiple copies of dihydrolipoyl acetyltransferase (E2p), which exhibits the shape of either a cube or a dodecahedron, depending on the source. The crystal structures of the 60-meric dihydrolipoyl acyltransferase cores of Bacillus stearothermophilus and Enterococcus faecalis pyruvate dehydrogenase complexes were determined and revealed a remarkably hollow dodecahedron with an outer diameter of approximately 237 A, 12 large openings of approximately 52 A diameter across the fivefold axes, and an inner cavity with a diameter of approximately 118 A. Comparison of cubic and dodecahedral E2p assemblies shows that combining the principles of quasi-equivalence formulated by Caspar and Klug [Caspar, D. L. & Klug, A. (1962) Cold Spring Harbor Symp. Quant. Biol. 27, 1-4] with strict Euclidean geometric considerations results in predictions of the major features of the E2p dodecahedron matching the observed features almost exactly.

A Real-Time Marker-Based Visual Sensor Based on a FPGA and a Soft Core Processor

PubMed Central

Tayara, Hilal; Ham, Woonchul; Chong, Kil To

2016-01-01

This paper introduces a real-time marker-based visual sensor architecture for mobile robot localization and navigation. A hardware acceleration architecture for post video processing system was implemented on a field-programmable gate array (FPGA). The pose calculation algorithm was implemented in a System on Chip (SoC) with an Altera Nios II soft-core processor. For every frame, single pass image segmentation and Feature Accelerated Segment Test (FAST) corner detection were used for extracting the predefined markers with known geometries in FPGA. Coplanar PosIT algorithm was implemented on the Nios II soft-core processor supplied with floating point hardware for accelerating floating point operations. Trigonometric functions have been approximated using Taylor series and cubic approximation using Lagrange polynomials. Inverse square root method has been implemented for approximating square root computations. Real time results have been achieved and pixel streams have been processed on the fly without any need to buffer the input frame for further implementation. PMID:27983714

A Real-Time Marker-Based Visual Sensor Based on a FPGA and a Soft Core Processor.

PubMed

Tayara, Hilal; Ham, Woonchul; Chong, Kil To

2016-12-15

This paper introduces a real-time marker-based visual sensor architecture for mobile robot localization and navigation. A hardware acceleration architecture for post video processing system was implemented on a field-programmable gate array (FPGA). The pose calculation algorithm was implemented in a System on Chip (SoC) with an Altera Nios II soft-core processor. For every frame, single pass image segmentation and Feature Accelerated Segment Test (FAST) corner detection were used for extracting the predefined markers with known geometries in FPGA. Coplanar PosIT algorithm was implemented on the Nios II soft-core processor supplied with floating point hardware for accelerating floating point operations. Trigonometric functions have been approximated using Taylor series and cubic approximation using Lagrange polynomials. Inverse square root method has been implemented for approximating square root computations. Real time results have been achieved and pixel streams have been processed on the fly without any need to buffer the input frame for further implementation.

Koopmans' analysis of chemical hardness with spectral-like resolution.

PubMed

Putz, Mihai V

2013-01-01

Three approximation levels of Koopmans' theorem are explored and applied: the first referring to the inner quantum behavior of the orbitalic energies that depart from the genuine ones in Fock space when the wave-functions' Hilbert-Banach basis set is specified to solve the many-electronic spectra of spin-orbitals' eigenstates; it is the most subtle issue regarding Koopmans' theorem as it brings many critics and refutation in the last decades, yet it is shown here as an irrefutable "observational" effect through computation, specific to any in silico spectra of an eigenproblem; the second level assumes the "frozen spin-orbitals" approximation during the extracting or adding of electrons to the frontier of the chemical system through the ionization and affinity processes, respectively; this approximation is nevertheless workable for great deal of chemical compounds, especially organic systems, and is justified for chemical reactivity and aromaticity hierarchies in an homologue series; the third and the most severe approximation regards the extension of the second one to superior orders of ionization and affinities, here studied at the level of chemical hardness compact-finite expressions up to spectral-like resolution for a paradigmatic set of aromatic carbohydrates.

Koopmans' Analysis of Chemical Hardness with Spectral-Like Resolution

PubMed Central

2013-01-01

Three approximation levels of Koopmans' theorem are explored and applied: the first referring to the inner quantum behavior of the orbitalic energies that depart from the genuine ones in Fock space when the wave-functions' Hilbert-Banach basis set is specified to solve the many-electronic spectra of spin-orbitals' eigenstates; it is the most subtle issue regarding Koopmans' theorem as it brings many critics and refutation in the last decades, yet it is shown here as an irrefutable “observational” effect through computation, specific to any in silico spectra of an eigenproblem; the second level assumes the “frozen spin-orbitals” approximation during the extracting or adding of electrons to the frontier of the chemical system through the ionization and affinity processes, respectively; this approximation is nevertheless workable for great deal of chemical compounds, especially organic systems, and is justified for chemical reactivity and aromaticity hierarchies in an homologue series; the third and the most severe approximation regards the extension of the second one to superior orders of ionization and affinities, here studied at the level of chemical hardness compact-finite expressions up to spectral-like resolution for a paradigmatic set of aromatic carbohydrates. PMID:23970834

Development and application of accurate analytical models for single active electron potentials

NASA Astrophysics Data System (ADS)

Miller, Michelle; Jaron-Becker, Agnieszka; Becker, Andreas

2015-05-01

The single active electron (SAE) approximation is a theoretical model frequently employed to study scenarios in which inner-shell electrons may productively be treated as frozen spectators to a physical process of interest, and accurate analytical approximations for these potentials are sought as a useful simulation tool. Density function theory is often used to construct a SAE potential, requiring that a further approximation for the exchange correlation functional be enacted. In this study, we employ the Krieger, Li, and Iafrate (KLI) modification to the optimized-effective-potential (OEP) method to reduce the complexity of the problem to the straightforward solution of a system of linear equations through simple arguments regarding the behavior of the exchange-correlation potential in regions where a single orbital dominates. We employ this method for the solution of atomic and molecular potentials, and use the resultant curve to devise a systematic construction for highly accurate and useful analytical approximations for several systems. Supported by the U.S. Department of Energy (Grant No. DE-FG02-09ER16103), and the U.S. National Science Foundation (Graduate Research Fellowship, Grants No. PHY-1125844 and No. PHY-1068706).

Effect of frozen storage on the structure and enzymatic activities of myofibrillar proteins of rabbit skeletal muscle.

PubMed

Kang, J O; Ito, T; Fukazawa, T

1983-01-01

The effect of frozen storage on the biochemical properties of myofibrils, and of their major constituents, actin and myosin, was investigated. Extractability of myofibrillar proteins increased slightly for 3 weeks during frozen storage of muscle, decreasing thereafter. The change in myofibrillar ATPase activity during frozen storage was consistent with that of a reconstituted acto-heavy meromyosin (HMM) complex prepared from frozen stored muscle at the same weight ratio of actin to myosin as in situ. However, myosin ATPase activity showed a different pattern of change when compared with myofibrillar ATPase activity. The maximum velocity of acto-HMM ATPase activity and the apparent dissociation constant of the acto-HMM complex decreased for 1 week during frozen storage, increasing thereafter, indicating that the affinity of actin for myosin was greatest in muscle which had been frozen for 1 week. Copyright © 1983. Published by Elsevier Ltd.

75 FR 22370 - Certain Frozen Warmwater Shrimp From Brazil, Ecuador, India, the People's Republic of China...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-28

...-893, A-549-822, A-552-802] Certain Frozen Warmwater Shrimp From Brazil, Ecuador, India, the People's... certain frozen warmwater shrimp from Brazil, Ecuador, India, the People's Republic of China, Thailand, and... Frozen and Canned Warmwater Shrimp From Ecuador, 69 FR 76913 (December 23, 2004) (Ecuador Final...

Abacus of Frozen Conflicts

DTIC Science & Technology

2010-06-01

NAVAL POSTGRADUATE SCHOOL MONTEREY, CALIFORNIA THESIS ABACUS OF FROZEN CONFLICTS by Reshad Karimov June 2010 Thesis...SUBTITLE Abacus of Frozen Conflicts 6. AUTHOR(S) Reshad Karimov 5. FUNDING NUMBERS 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Naval...PAGE INTENTIONALLY LEFT BLANK iii Approved for public release; distribution is unlimited ABACUS OF FROZEN CONFLICTS Reshad Karimov Research

76 FR 23277 - Certain Frozen Warmwater Shrimp From Brazil, India, the People's Republic of China, Thailand, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-26

... Fair Value: Certain Frozen and Canned Warmwater Shrimp From Brazil, 69 FR 76910 (December 23, 2004); Notice of Final Determination of Sales at Less Than Fair Value: Certain Frozen and Canned Warmwater... Fair Value and Negative Final Determination of Critical Circumstances: Certain Frozen and Canned...

21 CFR 161.176 - Frozen raw lightly breaded shrimp.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen raw lightly breaded shrimp. 161.176 Section 161.176 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Shellfish § 161.176 Frozen raw lightly breaded shrimp. Frozen raw lightly breaded shrimp complies with the...

21 CFR 161.176 - Frozen raw lightly breaded shrimp.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen raw lightly breaded shrimp. 161.176 Section 161.176 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Shellfish § 161.176 Frozen raw lightly breaded shrimp. Frozen raw lightly breaded shrimp complies with the...

21 CFR 146.137 - Frozen orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen orange juice. 146.137 Section 146.137 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135...

75 FR 69628 - Non-Frozen Apple Juice Concentrate From the People's Republic of China: Final Results of Sunset...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-15

... DEPARTMENT OF COMMERCE International Trade Administration [A-570-855] Non-Frozen Apple Juice... order on non-frozen apple juice concentrate from the People's Republic of China (``PRC''). Because the... June 5, 2000, the Department issued an antidumping duty order on certain non-frozen apple juice...

75 FR 29726 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Notice of Partial Rescission...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-27

... DEPARTMENT OF COMMERCE International Trade Administration [A-552-801] Certain Frozen Fish Fillets... antidumping duty order on certain frozen fish fillets from the Socialist Republic of Vietnam (``Vietnam''). See Notice of Antidumping Duty Order: Certain Frozen Fish Fillets From the Socialist Republic of...

76 FR 20626 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Extension of Time Limit for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

... DEPARTMENT OF COMMERCE International Trade Administration [A-552-801] Certain Frozen Fish Fillets... and requests for revocation in part for certain frozen fish fillets from the Socialist Republic of... administrative reviews and requests for revocation in part for certain frozen fish fillets from the Socialist...

21 CFR 146.120 - Frozen concentrate for lemonade.

Code of Federal Regulations, 2011 CFR

2011-04-01

... lemonade is the frozen food prepared from one or both of the lemon juice ingredients specified in paragraph... percent by weight. (b) The lemon juice ingredients referred to in paragraph (a) of this section are: (1) Lemon juice or frozen lemon juice or a mixture of these. (2) Concentrated lemon juice or frozen...

78 FR 39708 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Final Results of Antidumping...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-02

... From the Socialist Republic of Vietnam: Final Results of Antidumping Duty New Shipper Reviews; 2011... frozen fish fillets (``frozen fish fillets'') from the Socialist Republic of Vietnam (``Vietnam'').\\1...'' section of this notice. \\1\\ See Certain Frozen Fish Fillets From the Socialist Republic of Vietnam...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 3 2011-01-01 2011-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 3 2012-01-01 2012-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 3 2014-01-01 2014-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 3 2013-01-01 2013-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

The Organic Content of the Tagish Lake Meteorite

NASA Technical Reports Server (NTRS)

Pizzarello, Sandra; Huang, Yongsong; Becker, Luann; Poreda, Robert J.; Nieman, Ronald A.; Cooper, George; Williams, Michael

2001-01-01

The Tagish Lake meteorite felt last year on a frozen take in Canada and may provide the most pristine material of its kind. Analyses have now shown this carbonaceous chondrite to contain a suite of soluble organic compounds (approximately 100 parts per million) that includes mono- and dicarboxylic acids, dicarboximides, pyridine carboxylic acids, a sulfonic acid, and both aliphatic and aromatic hydrocarbons. The insoluble carbon exhibits exclusive aromatic character, deuterium enrichment, and fullerenes containing 'planetary' helium and argon. The findings provide insight into an outcome of early solar chemical evolution that differs from any seen so far in meteorites.

Oxygen Mass Transfer Coefficient (k sub L a’) Consideration for Scale-Up Fermentation Systems at the Biotechnology Laboratory U.S. Army Edgewood Chemical Biological Center

DTIC Science & Technology

2011-02-01

30 °C Stock vials were prepared following standard microbial protocol. A single colony from the overnight nutrient agar plate was inoculated into...500-mL seed cultures were prepared by inoculating with 2 vials of frozen stock and incubating at 30 °C and 200 rpm until the OD reached approximately...and OD profiles vs. elapsed fermentation time ( EFT ) from 20- and 1000-L scales are shown in Figure 9. In both runs, no DO limitation was apparent, and

Estimating the Distribution and Production of Microplankton in a Coastal Upwelling Front from the Cellular Content of Guanosine-5’-Triphosphate and Adenosine-5’-Triphosphate.

DTIC Science & Technology

1981-09-01

through a microfine glass fiber tilter (Reeve Angel 984-H with a pore size of approximately 0.45 pnm). This...buffer (pH 7.7) in order to extract the nucleotides present in the water sample. The test tubes containing the extracts were labeled and stored frozen...into a series of disposable glass cuvettes (12x75mm) and placed in a test tube rack in an incubating water bath at 300 C. Each tube was allowed to

Geometry of the Nojima fault at Nojima-Hirabayashi, Japan - I. A simple damage structure inferred from borehole core permeability

USGS Publications Warehouse

Lockner, David A.; Tanaka, Hidemi; Ito, Hisao; Ikeda, Ryuji; Omura, Kentaro; Naka, Hisanobu

2009-01-01

The 1995 Kobe (Hyogo-ken Nanbu) earthquake, M = 7.2, ruptured the Nojima fault in southwest Japan. We have studied core samples taken from two scientific drillholes that crossed the fault zone SW of the epicentral region on Awaji Island. The shallower hole, drilled by the Geological Survey of Japan (GSJ), was started 75 m to the SE of the surface trace of the Nojima fault and crossed the fault at a depth of 624 m. A deeper hole, drilled by the National Research Institute for Earth Science and Disaster Prevention (NIED) was started 302 m to the SE of the fault and crossed fault strands below a depth of 1140 m. We have measured strength and matrix permeability of core samples taken from these two drillholes. We find a strong correlation between permeability and proximity to the fault zone shear axes. The half-width of the high permeability zone (approximately 15 to 25 m) is in good agreement with the fault zone width inferred from trapped seismic wave analysis and other evidence. The fault zone core or shear axis contains clays with permeabilities of approximately 0.1 to 1 microdarcy at 50 MPa effective confining pressure (10 to 30 microdarcy at in situ pressures). Within a few meters of the fault zone core, the rock is highly fractured but has sustained little net shear. Matrix permeability of this zone is approximately 30 to 60 microdarcy at 50 MPa effective confining pressure (300 to 1000 microdarcy at in situ pressures). Outside this damage zone, matrix permeability drops below 0.01 microdarcy. The clay-rich core material has the lowest strength with a coefficient of friction of approximately 0.55. Shear strength increases with distance from the shear axis. These permeability and strength observations reveal a simple fault zone structure with a relatively weak fine-grained core surrounded by a damage zone of fractured rock. In this case, the damage zone will act as a high-permeability conduit for vertical and horizontal flow in the plane of the fault. The fine-grained core region, however, will impede fluid flow across the fault.

Frozen-Thawed Embryo Transfer Cycles Have a Lower Incidence of Ectopic Pregnancy Compared With Fresh Embryo Transfer Cycles.

PubMed

Zhang, Xinyu; Ma, Caihong; Wu, Zhangxin; Tao, Liyuan; Li, Rong; Liu, Ping; Qiao, Jie

2017-01-01

To evaluate the risk of ectopic pregnancy of embryo transfer. A retrospective cohort study on the incidence of ectopic pregnancy in fresh and frozen-thawed embryo transfer cycles from January 1 st , 2010, to January 1 st , 2015. Infertile women undergoing frozen-thawed transfer cycles or fresh transfer cycles. In-vitro fertilization, fresh embryo transfer, frozen-thawed embryo transfer, ectopic pregnancy. Ectopic pregnancy rate and clinical pregnancy rate. A total of 69 756 in vitro fertilization-embryo transfer cycles from 2010 to 2015 were analyzed, including 45 960 (65.9%) fresh and 23 796 (34.1%) frozen-thawed embryo transfer cycles. The clinical pregnancy rate per embryo transfer was slightly lower in fresh embryo transfer cycles compared with frozen-thawed embryo transfer cycles (40.8% vs 43.1%, P < .001). Frozen-thawed embryo transfer is associated with a lower incidence of ectopic pregnancy per clinical pregnancy, compared with fresh embryo transfers (odds ratio = 0.31; 95% confidence interval = 0.24-0.39). Female age and body mass index have no influence on ectopic pregnancy. In the frozen-thawed embryo transfer cycles, blastocyst transfer shows a significantly lower incidence of ectopic pregnancy (0.8% vs 1.8%, P = .002) in comparison with day 3 cleavage embryo transfer. The risk of ectopic pregnancy is lower in frozen-thawed embryo transfer cycles than fresh embryo transfer cycles, and blastocyst transfer could further decrease the ectopic pregnancy rate in frozen-thawed embryo transfer cycles.

Utility of intraoperative frozen sections in surgical decision making for acute invasive fungal rhinosinusitis.

PubMed

Papagiannopoulos, Peter; Lin, Diana Murro; Al-Khudari, Samer; Rajan, Kumar; Reddy, Swathi; Gattuso, Paulo; Tajudeen, Bobby; Batra, Pete S

2017-05-01

Acute invasive fungal rhinosinusitis (AIFRS) represents a fulminant, potentially fatal, disease process in immunocompromised patients. The diagnosis often rests on high index of clinical suspicion, with relative paucity of data on the diagnostic and therapeutic implications of intraoperative frozen sections. Retrospective review was performed for 18 cases undergoing endoscopic sinus surgery for AIFRS. Reliability of intraoperative frozen section diagnosis was evaluated for all patients using final pathology as the gold standard. A total of 66 frozen sections were performed. Diagnostic accuracy of frozen sections illustrated sensitivity of 72.7% (95% confidence interval [CI], 0.57 to 0.85), specificity of 100% (95% CI, 0.85 to 1.00), positive predictive value (PPV) of 100% (95% CI, 0.89 to 1.00), and negative predictive value (NPV) of 64.7% (95% CI, 0.46 to 0.80). There was no statistically significant difference in sensitivity of frozen sections in cases of Mucor and Aspergillus at 68.8%% and 76.2%, respectively (p = 0.61). This study represents the largest series assessing the diagnostic accuracy of frozen section analysis in AIFRS. Frozen section analysis is an effective tool for guiding intraoperative decision making in patients with AIFRS with a high PPV. A Low NPV underscores the importance of clinical suspicion and intraoperative decision making based on endoscopic findings when negative frozen section results are encountered. Further, frozen section analysis appears to be equally effective in detecting either Mucor or Aspergillus. © 2017 ARS-AAOA, LLC.

Deuterated methanol map towards L1544

NASA Astrophysics Data System (ADS)

Chacón-Tanarro, A.; Caselli, P.; Bizzocchi, L.; Pineda, J. E.; Spezzano, S.; Giuliano, B. M.; Lattanzi, V.; Punanova, A.

Pre-stellar cores are self-gravitating starless dense cores with clear signs of contraction and chemical evolution (Crapsi et al. 2005), considered to represent the initial conditions in the process of star formation (Caselli & Ceccarelli 2012). Theoretical studies predict that CO is one of the precursors of complex organic molecules (COMs) during this cold and dense phase (Tielens et al. 1982; Watanabe et al. 2002). Moreover, when CO starts to deplete onto dust grains (at densities of a few 104 cm-3), the formation of deuterated species is enhanced, as CO accelerates the destruction of important precursors of deuterated molecules (Dalgarno & Lepp 1984). Here, we present the CH_2DOH/CH_3OH column density map toward the pre-stellar core L1544 (Chacón-Tanarro et al., in prep.), taken with the IRAM 30 m antenna. The results are compared with the C17O (1-0) distribution across L1544. As methanol is formed on dust grains via hydrogenation of frozen-out CO, this work allows us to measure the deuteration on surfaces and compared it with gas phase deuteration, as well as CO freeze-out and dust properties. This is important to shed light on the basic chemical processes just before the formation of a stellar system.

Assessment of frozen storage duration effect on quality characteristics of various horse muscles.

PubMed

Seong, Pil Nam; Seo, Hyun Woo; Kim, Jin-Hyoung; Kang, Geun Ho; Cho, Soo-Hyun; Chae, Hyun Seok; Park, Beom Young; Van Ba, Hoa

2017-12-01

The study aimed at assessing the effects of frozen storage duration on quality characteristics, lipid oxidation and sensory quality of various horse muscles. Five representative muscles: longissimus dorsi (LD), gluteus medius (GM), semimembranosus (SM), biceps femoris (BF), and triceps brachii (TB) at 24 h post-mortem obtained from 28-mo-old Jeju female breed horses (n = 8) were used in the present investigation. The muscles were vacuum-packaged and frozen at -20°C for 120, 240, and 360 days. All the samples were analyzed for thawing and cooking losses, pH, Warner-Bratzler shear forces (WBSF), color traits, total volatile basic nitrogen (TVBN), thiobarbituric acid reactive substances (TBARS) and sensory traits. The muscle samples analyzed on day 0 of frozen storage (fresh, non-frozen) were used for comparison. Results revealed that thawing and cooking losses significantly (p<0.05) increased in all the muscles after 120 days and then remained unchanged up to 360 days of frozen storage. The TBARS and TVBN contents significantly increased as increasing frozen storage time up to 360 days (p<0.05). While, significant decreases in WBSF values were observed for all the muscles with increased frozen storage time (p<0.05). Frozen storage variously affected the color traits of the muscles for instance; the redness of LD, GM, and BF muscles showed a decreasing tendency during frozen storage while it was not changed in TB and SM muscles. Furthermore, the frozen storage did not produce detrimental effects on sensory quality as it did not cause flavor and juiciness defects whereas it partially improved the tenderness of all the muscles studied. Based on the results obtained from our work, it is concluded that frozen storage could be applied to increase the long-term shelf life of horsemeat while still retaining its sensory quality.

Description of core samples returned by Apollo 12

NASA Technical Reports Server (NTRS)

Lindsay, J. F.; Fryxell, R.

1971-01-01

Three core samples were collected by the Apollo 12 astronauts. Two are single cores, one of which (sample 12026) was collected close to the lunar module during the first extravehicular activity period and is 19.3 centimeters long. The second core (sample 12027) was collected at Sharp Crater during the second extravehicular activity period and is 17.4 centimeters long. The third sample is a double core (samples 12025 and 12028), which was collected near Halo Crater during the second extravehicular activity period. Unlike the other cores, the double-drive-tube core sample has complex layering with at least 10 clearly defined stratigraphic units. This core sample is approximately 41 centimeters long.

Accretion rate of extraterrestrial matter: Iridium deposited over the last 70 million years

NASA Technical Reports Server (NTRS)

Kyte, Frank T.

1988-01-01

In order to quantify the accretion rate of extraterrestrial matter during the Cenozoic, Ir concentrations were measured in a continuous series of 450 samples across most of the length of piston core LL44-GPC3. LL44-GPC3 is a 25-meter-long, large-diameter piston core of abyssal clay from the central North Pacific. This core contains a nearly continuous record of sedimentation over the last 70 Ma, as this site migrated from a region near the Equator in the late Cretaceous to its present position north of Hawaii. The first-cut survey across the core is nearing completion, and all of the conclusions of the earlier study, in which was reported the concentrations of Ir, Co, and Sb across 9 meters of this core, remain unchanged. The only strongly enhanced Ir concentrations occur at the Cretaceous-Tertiary (K-T) boundary and outside the K-T boundary Ir correlates well with Co, a terrestrial element which is largely present in hydrogenous ferromanganese oxide precipitates from seawater. Concentrations of both elements appear to be inversely correlated with the sedimentation rate. Although the K-T Ir anomaly is unique in magnitude in this core, there are several small bumps in the Ir profile which may reflect smaller accretionary events. The most promising Ir enhancement was observed in a 30 cm section approximately 1 m below the K-T boundary. Preliminary data suggest deposition of an excess across this interval at a time estimate to be approximate 1 Ma before the K-T impact event, but there is insufficient evidence at present to prove that this reflects enhanced accretion of extraterrestrial matter. A detailed model is being prepared of the chemical record of sedimentation in this core using a combined database of 39 elements in approximately 450 samples across the Cenozoic. Preliminary working model indicates that the only sedimentary sources which contribute significantly to the Ir budget in this core are the hydrogenous precipitates and extraterrestrial particulates.

Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ atomic force microscopy.

PubMed

Scheuring, Simon; Busselez, Johan; Lévy, Daniel

2005-01-14

We have studied photosynthetic membranes of wild type Rhodobacter blasticus, a closely related strain to the well studied Rhodobacter sphaeroides, using atomic force microscopy. High-resolution atomic force microscopy topographs of both cytoplasmic and periplasmic surfaces of LH2 and RC-LH1-PufX (RC, reaction center) complexes were acquired in situ. The LH2 is a nonameric ring inserted into the membrane with the 9-fold axis perpendicular to the plane. The core complex is an S-shaped dimer composed of two RCs, each encircled by 13 LH1 alpha/beta-heterodimers, and two PufXs. The LH1 assembly is an open ellipse with a topography-free gap of approximately 25 A. The two PufXs, one of each core, are located at the dimer center. Based on our data, we propose a model of the core complex, which provides explanation for the PufX-induced dimerization of the Rhodobacter core complex. The QB site is located facing a approximately 25-A wide gap within LH1, explaining the PufX-favored quinone passage in and out of the core complex.

75 FR 61127 - Non-Frozen Apple Juice Concentrate from the People's Republic of China: Extension of Time Limit...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-04

... DEPARTMENT OF COMMERCE International Trade Administration [A-570-855] Non-Frozen Apple Juice... order on non-frozen apple juice concentrate from the People's Republic of China (``PRC'').\\1\\ This... currently due no later than October 28, 2010. \\1\\ See Certain Non-Frozen Apple Juice Concentrate from the...

75 FR 81564 - Certain Non-Frozen Apple Juice Concentrate From the People's Republic of China: Final Results of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-28

... DEPARTMENT OF COMMERCE International Trade Administration [A-570-855] Certain Non-Frozen Apple... order covering certain non-frozen apple juice concentrate from the People's Republic of China. See...: Certain Non-Frozen Apple Juice Concentrate From the People's Republic of China, 65 FR 35606 (June 5, 2000...

40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2013 CFR

2013-07-01

... mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2014 CFR

2014-07-01

... mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2010 CFR

2010-07-01

... mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2011 CFR

2011-07-01

... mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

Axisymmetric simulations of magnetorotational core collapse: approximate inclusion of general relativistic effects

NASA Astrophysics Data System (ADS)

Obergaulinger, M.; Aloy, M. A.; Dimmelmeier, H.; Müller, E.

2006-10-01

We continue our investigations of the magnetorotational collapse of stellar cores by discussing simulations performed with a modified Newtonian gravitational potential that mimics general relativistic effects. The approximate TOV gravitational potential used in our simulations captures several basic features of fully relativistic simulations quite well. In particular, it is able to correctly reproduce the behavior of models that show a qualitative change both of the dynamics and the gravitational wave signal when switching from Newtonian to fully relativistic simulations. For models where the dynamics and gravitational wave signals are already captured qualitatively correctly by a Newtonian potential, the results of the Newtonian and the approximate TOV models differ quantitatively. The collapse proceeds to higher densities with the approximate TOV potential, allowing for a more efficient amplification of the magnetic field by differential rotation. The strength of the saturation fields (˜ 1015 ~ G at the surface of the inner core) is a factor of two to three higher than in Newtonian gravity. Due to the more efficient field amplification, the influence of magnetic fields is considerably more pronounced than in the Newtonian case for some of the models. As in the Newtonian case, sufficiently strong magnetic fields slow down the core's rotation and trigger a secular contraction phase to higher densities. More clearly than in Newtonian models, the collapsed cores of these models exhibit two different kinds of shock generation. Due to magnetic braking, a first shock wave created during the initial centrifugal bounce at subnuclear densities does not suffice for ejecting any mass, and the temporarily stabilized core continues to collapse to supranuclear densities. Another stronger shock wave is generated during the second bounce as the core exceeds nuclear matter density. The gravitational wave signal of these models does not fit into the standard classification. Therefore, in the first paper of this series we introduced a new type of gravitational wave signal, which we call type IV or “magnetic type”. This signal type is more frequent for the approximate relativistic potential than for the Newtonian one. Most of our weak-field models are marginally detectable with the current LIGO interferometer for a source located at a distance of 10 kpc. Strongly magnetized models emit a substantial fraction of their GW power at very low frequencies. A flat spectrum between 10 Hz and ⪉ 100 kHz denotes the generation of a jet-like hydromagnetic outflow.

Horizontal Resorption of Fresh-Frozen Corticocancellous Bone Blocks in the Reconstruction of the Atrophic Maxilla at 5 Months

PubMed Central

Pereira, Eugénio; Messias, Ana; Dias, Ricardo; Judas, Fernando; Salvoni, Alexander; Guerra, Fernando

2015-01-01

Background Reliable implant-supported rehabilitation of an alveolar ridge needs sufficient volume of bone. In order to achieve a prosthetic-driven positioning, bone graft techniques may be required. Purpose This prospective cohort study aims to clinically evaluate the amount of resorption of corticocancellous fresh-frozen allografts bone blocks used in the reconstruction of the severe atrophic maxilla. Materials and Methods Twenty-two partial and totally edentulous patients underwent bone augmentation procedures with fresh-frozen allogenous blocks from the iliac crest under local anesthesia. Implants were inserted into the grafted sites after a healing period of 5 months. Final fixed prosthesis was delivered ± 4 months later. Ridge width analysis and measurements were performed with a caliper before and after grafting and at implant insertion. Bone biopsies were performed in 16 patients. Results A total of 98 onlay block allografts were used in 22 patients with an initial mean alveolar ridge width of 3.41 ± 1.36 mm. Early exposure of blocks was observed in four situations and one of these completely resorbed. Mean horizontal bone gain was 3.63 ± 1.28 mm (p < .01). Mean buccal bone resorption between allograph placement and the reopening stage was 0.49 ± 0.54 mm, meaning approximately 7.1% (95% confidence interval: [5.6%, 8.6%]) of total ridge width loss during the integration period. One hundred thirty dental implants were placed with good primary stability (≥ 30 Ncm). Four implants presented early failure before the prosthetic delivery (96.7% implant survival). All patients were successfully rehabilitated. Histomorphometric analysis revealed 20.9 ± 5.8% of vital bone in close contact to the remaining grafted bone. A positive strong correlation (adjusted R2 = 0.44, p = .003) was found between healing time and vital bone percentage. Conclusions Augmentation procedures performed using fresh-frozen allografts from the iliac crest are a suitable alternative in the reconstruction of the atrophic maxilla with low resorption rate at 5 months, allowing proper stability of dental implants followed by fixed prosthetic rehabilitation. PMID:25346211

Cryosurgery Planning Using Bubble Packing in 3D

PubMed Central

Tanaka, Daigo; Shimada, Kenji; Rossi, Michael R.; Rabin, Yoed

2008-01-01

As part of an ongoing project to develop automated tools for cryosurgery planning, the current study focuses on the development of a 3D bubble packing method. A proof-of-concept for the new method is demonstrated on five prostate models, reconstructed from ultrasound images. The new method is a modification of an established method in 2D. Ellipsoidal bubbles are packed in the volume of the prostate in the current study; such bubbles can be viewed as a first-order approximation of a frozen region around a single cryoprobe. When all cryoprobes are inserted to the same depth, optimum planning was found to occur at about 60% of the length of the prostate (measured from its apex), which leads to cooling of approximately 75% of the prostate volume below a specific temperature threshold of −22°C. Bubble packing has the potential to dramatically reduce the run time for automated planning. PMID:17963095

Cryosurgery planning using bubble packing in 3D.

PubMed

Tanaka, Daigo; Shimada, Kenji; Rossi, Michael R; Rabin, Yoed

2008-04-01

As part of an ongoing project to develop automated tools for cryosurgery planning, the current study focuses on the development of a 3D bubble packing method. A proof-of-concept for the new method is demonstrated on five prostate models, reconstructed from ultrasound images. The new method is a modification of an established method in 2D. Ellipsoidal bubbles are packed in the volume of the prostate in the current study; such bubbles can be viewed as a first-order approximation of a frozen region around a single cryoprobe. When all cryoprobes are inserted to the same depth, optimum planning was found to occur at about 60% of the length of the prostate (measured from its apex), which leads to cooling of approximately 75% of the prostate volume below a specific temperature threshold of - 22 degrees C. Bubble packing has the potential to dramatically reduce the run time for automated planning.

ANALYZING NUMERICAL ERRORS IN DOMAIN HEAT TRANSPORT MODELS USING THE CVBEM.

USGS Publications Warehouse

Hromadka, T.V.; ,

1985-01-01

Besides providing an exact solution for steady-state heat conduction processes (Laplace Poisson equations), the CVBEM (complex variable boundary element method) can be used for the numerical error analysis of domain model solutions. For problems where soil water phase change latent heat effects dominate the thermal regime, heat transport can be approximately modeled as a time-stepped steady-state condition in the thawed and frozen regions, respectively. The CVBEM provides an exact solution of the two-dimensional steady-state heat transport problem, and also provides the error in matching the prescribed boundary conditions by the development of a modeling error distribution or an approximative boundary generation. This error evaluation can be used to develop highly accurate CVBEM models of the heat transport process, and the resulting model can be used as a test case for evaluating the precision of domain models based on finite elements or finite differences.

Do Titan's Mountains Betray the Late Acquisition of its Current Atmosphere

NASA Technical Reports Server (NTRS)

Moore, Jeffrey Morgan; Nimmo, F.

2011-01-01

Titan may have acquired its massive atmosphere relatively recently in solar system history [1,2,3,4]. Prior to that time, Titan would have been nearly airless, with its volatiles frozen or sequestered. Present-day Titan experiences only small (approximately 4 K) pole-to-equator variations, owing to efficient heat transport via the thick atmosphere [5]; these temperature variations would have been much larger (approximately 20 K) in the absence of an atmosphere. If Titan's ice shell is conductive, the change in surface temperature associated with the development of an atmosphere would have led to changes in shell thickness. In particular, the poles would move down (inducing compression) while the equator would move up. Figure 1 shows the predicted change in surface elevation as a result of the change in surface temperature, using the numerical conductive shell thickness model of [6

High-resolution Statistics of Solar Wind Turbulence at Kinetic Scales Using the Magnetospheric Multiscale Mission

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chasapis, Alexandros; Matthaeus, W. H.; Parashar, T. N.

Using data from the Magnetospheric Multiscale (MMS) and Cluster missions obtained in the solar wind, we examine second-order and fourth-order structure functions at varying spatial lags normalized to ion inertial scales. The analysis includes direct two-spacecraft results and single-spacecraft results employing the familiar Taylor frozen-in flow approximation. Several familiar statistical results, including the spectral distribution of energy, and the sale-dependent kurtosis, are extended down to unprecedented spatial scales of ∼6 km, approaching electron scales. The Taylor approximation is also confirmed at those small scales, although small deviations are present in the kinetic range. The kurtosis is seen to attain verymore » high values at sub-proton scales, supporting the previously reported suggestion that monofractal behavior may be due to high-frequency plasma waves at kinetic scales.« less

Preliminary paleontologic report on cores 19A and 19B, from Russell Bank, Everglades National Park, Florida Bay

USGS Publications Warehouse

Brewster-Wingard, G. L.; Ishman, S.E.; Willard, D.A.; Edwards, L.E.; Holmes, C.W.

1997-01-01

The fauna and flora preserved in two cores, 19A and 19B, from the south side of Russell Bank (N 25 03.831', W 80 37.486') in north-central Florida Bay, Everglades National Park, Florida, record a history of environmental change over the last century. The benthic foraminifera and molluscs indicate fluctuating salinities with increasing average salinity upcore in core 19B. Shifts from low salinity (12-15 ppt) to higher average salinity (30 ppt) occurred at 70-66 cm and 24-18 cm in core 19B (approximately 1937-1940 and 1975-1980). The inverse, shifts from periods of higher average salinity to periods of lower salinity, occurred at 140 cm, 90 cm, and 42 cm (approximately 1880, 1921, and 1960). Significant changes in the molluscan fauna indicative of specific substrate types occur at 88 cm, 68 cm, and 22 cm. The lower portion of the core is dominated by a mixture of sediment and grass dwellers, the middle portion by sediment dwellers, and the upper portion of the core by grass and finally grass and algae dwellers. Changes occur in the floral assemblages in core 19A, but the significance of these changes is unclear. Three subtle shifts occur in the pollen assemblages indicating the onshore vegetation was responding to some environmental factor. Two peaks in dinocyst abundance occur in core 19A, but the composition of dinocyst assemblages remains relatively stable throughout the core. Correspondence between changes in salinity and onshore vegetation changes is consistent with results from previous cores. The pattern of increased salinity upcore is consistent with patterns seen in core T24 from the mouth of Taylor Creek and in core 6A from Bob Allen mudbank.

Orthogonality of embedded wave functions for different states in frozen-density embedding theory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zech, Alexander; Wesolowski, Tomasz A.; Aquilante, Francesco

2015-10-28

Other than lowest-energy stationary embedded wave functions obtained in Frozen-Density Embedding Theory (FDET) [T. A. Wesolowski, Phys. Rev. A 77, 012504 (2008)] can be associated with electronic excited states but they can be mutually non-orthogonal. Although this does not violate any physical principles — embedded wave functions are only auxiliary objects used to obtain stationary densities — working with orthogonal functions has many practical advantages. In the present work, we show numerically that excitation energies obtained using conventional FDET calculations (allowing for non-orthogonality) can be obtained using embedded wave functions which are strictly orthogonal. The used method preserves the mathematicalmore » structure of FDET and self-consistency between energy, embedded wave function, and the embedding potential (they are connected through the Euler-Lagrange equations). The orthogonality is built-in through the linearization in the embedded density of the relevant components of the total energy functional. Moreover, we show formally that the differences between the expectation values of the embedded Hamiltonian are equal to the excitation energies, which is the exact result within linearized FDET. Linearized FDET is shown to be a robust approximation for a large class of reference densities.« less

Dynamical Response of Networks Under External Perturbations: Exact Results

NASA Astrophysics Data System (ADS)

Chinellato, David D.; Epstein, Irving R.; Braha, Dan; Bar-Yam, Yaneer; de Aguiar, Marcus A. M.

2015-04-01

We give exact statistical distributions for the dynamic response of influence networks subjected to external perturbations. We consider networks whose nodes have two internal states labeled 0 and 1. We let nodes be frozen in state 0, in state 1, and the remaining nodes change by adopting the state of a connected node with a fixed probability per time step. The frozen nodes can be interpreted as external perturbations to the subnetwork of free nodes. Analytically extending and to be smaller than 1 enables modeling the case of weak coupling. We solve the dynamical equations exactly for fully connected networks, obtaining the equilibrium distribution, transition probabilities between any two states and the characteristic time to equilibration. Our exact results are excellent approximations for other topologies, including random, regular lattice, scale-free and small world networks, when the numbers of fixed nodes are adjusted to take account of the effect of topology on coupling to the environment. This model can describe a variety of complex systems, from magnetic spins to social networks to population genetics, and was recently applied as a framework for early warning signals for real-world self-organized economic market crises.

Ligand protons in a frozen solution of copper histidine relax via a T1e-driven three-spin mechanism

NASA Astrophysics Data System (ADS)

Stoll, S.; Epel, B.; Vega, S.; Goldfarb, D.

2007-10-01

Davies electron-nuclear double resonance spectra can exhibit strong asymmetries for long mixing times, short repetition times, and large thermal polarizations. These asymmetries can be used to determine nuclear relaxation rates in paramagnetic systems. Measurements of frozen solutions of copper(L-histidine)2 reveal a strong field dependence of the relaxation rates of the protons in the histidine ligand, increasing from low (g‖) to high (g⊥) field. It is shown that this can be attributed to a concentration-dependent T1e-driven relaxation process involving strongly mixed states of three spins: the histidine proton, the Cu(II) electron spin of the same complex, and another distant electron spin with a resonance frequency differing from the spectrometer frequency approximately by the proton Larmor frequency. The protons relax more efficiently in the g⊥ region, since the number of distant electrons able to participate in this relaxation mechanism is higher than in the g‖ region. Analytical expressions for the associated nuclear polarization decay rate Teen-1 are developed and Monte Carlo simulations are carried out, reproducing both the field and the concentration dependences of the nuclear relaxation.

Super-Eddington Accretion in Tidal Disruption Events: the Impact of Realistic Fallback Rates on Accretion Rates

NASA Astrophysics Data System (ADS)

Wu, Samantha; Coughlin, Eric R.; Nixon, Chris

2018-04-01

After the tidal disruption of a star by a massive black hole, disrupted stellar debris can fall back to the hole at a rate significantly exceeding its Eddington limit. To understand how black hole mass affects the duration of super-Eddington accretion in tidal disruption events, we first run a suite of simulations of the disruption of a Solar-like star by a supermassive black hole of varying mass to directly measure the fallback rate onto the hole, and we compare these fallback rates to the analytic predictions of the "frozen-in" model. Then, adopting a Zero-Bernoulli Accretion flow as an analytic prescription for the accretion flow around the hole, we investigate how the accretion rate onto the black hole evolves with the more accurate fallback rates calculated from the simulations. We find that numerically-simulated fallback rates yield accretion rates onto the hole that can, depending on the black hole mass, be nearly an order of magnitude larger than those predicted by the frozen-in approximation. Our results place new limits on the maximum black hole mass for which super-Eddington accretion occurs in tidal disruption events.

A COMPARISON OF EXPERIMENTS AND THREE-DIMENSIONAL ANALYSIS TECHNIQUES. PART I. UNPOISONED UNIFORM SLAB CORE WITH A PARTIALLY INSERTED HAFNIUM ROD

DOE Office of Scientific and Technical Information (OSTI.GOV)

Renzi, N.E.; Roseberry, R.J.

>The experimental measurements and nuclear analysis of a uniformly loaded, unpoisoned slab core with a partially insented hafnium rod are described. Comparisons of experimental data with calculated results of the UFO code and flux synthesis techniques are given. It was concluded that one of the flux synthesis techniques and the UFO code are able to predict flux distributions to within approximately 5% of experiment for most cases. An error of approximately 10% was found in the synthesis technique for a channel near the partially inserted rod. The various calculations were able to predict neutron pulsed shutdowns to only approximately 30%.more » (auth)« less

Effect of Age and Bulls on Fresh Semen Quality and Frozen Semen Production of Holstein Bulls in Indonesia

NASA Astrophysics Data System (ADS)

Argiris, A.; Ondho, Y. S.; Santoso, S. I.; Kurnianto, E.

2018-02-01

Artificial Insemination is a compatible method of reproduction in an effort to increase dairy productivity. Artificial Insemination Center as a producer of frozen semen was required to maximize bulls in producing high quality frozen semen optimally. The purpose of this research was to determine effect of age and bulls on fresh semen quality and frozen semen production of Holstein bulls in Indonesia. The research was conducted at Lembang and Singosari AI Centers. The material used were 24.634 data of qualified fresh semen and frozen semen production from 81 Holstein Bulls aged 1-9 years that used as frozen semen producer in period of 2008 to 2016. The variables observed in this research were data of age of bulls, fresh semen volume (mL); sperm motility (%); mass movement; concentrations (million/mL) and frozen semen doses at each production at age of bulls. Nested design was applied to obtain and analyze data. Results showed that Age and bulls have significant effect (P<0,01) to volume, mass, motility, concentration and frozen semen production. Increasing the age of bulls resulted in increase semen volume until 7-year-old, while semen concentration decreased from 3 years old with increasing age. Frozen semen production, mass movement and motility shown the same relative value on 3-9 years old except on 1 to 2 years old had increase. Bulls would produce frozen semen optimally on 3-9 years old. Indeed, with knowledge of this factor, AI Centre might adapt management of AI bulls to improve semen production.

Flow throughout the Earth's core inverted from geomagnetic observations and numerical dynamo models

NASA Astrophysics Data System (ADS)

Aubert, Julien

2013-02-01

This paper introduces inverse geodynamo modelling, a framework imaging flow throughout the Earth's core from observations of the geomagnetic field and its secular variation. The necessary prior information is provided by statistics from 3-D and self-consistent numerical simulations of the geodynamo. The core method is a linear estimation (or Kalman filtering) procedure, combined with standard frozen-flux core surface flow inversions in order to handle the non-linearity of the problem. The inversion scheme is successfully validated using synthetic test experiments. A set of four numerical dynamo models of increasing physical complexity and similarity to the geomagnetic field is then used to invert for flows at single epochs within the period 1970-2010, using data from the geomagnetic field models CM4 and gufm-sat-Q3. The resulting core surface flows generally provide satisfactory fits to the secular variation within the level of modelled errors, and robustly reproduce the most commonly observed patterns while additionally presenting a high degree of equatorial symmetry. The corresponding deep flows present a robust, highly columnar structure once rotational constraints are enforced to a high level in the prior models, with patterns strikingly similar to the results of quasi-geostrophic inversions. In particular, the presence of a persistent planetary scale, eccentric westward columnar gyre circling around the inner core is confirmed. The strength of the approach is to uniquely determine the trade-off between fit to the data and complexity of the solution by clearly connecting it to first principle physics; statistical deviations observed between the inverted flows and the standard model behaviour can then be used to quantitatively assess the shortcomings of the physical modelling. Such deviations include the (i) westwards and (ii) hemispherical character of the eccentric gyre. A prior model with angular momentum conservation of the core-mantle inner-core system, and gravitational coupling of reasonable strength between the mantle and the inner core, is shown to produce enough westward drift to resolve statistical deviation (i). Deviation (ii) is resolved by a prior with an hemispherical buoyancy release at the inner-core boundary, with excess buoyancy below Asia. This latter result suggests that the recently proposed inner-core translational instability presently transports the solid inner-core material westwards, opposite to the seismologically inferred long-term trend but consistently with the eccentricity of the geomagnetic dipole in recent times.

40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2012 CFR

2012-07-01

... fluid mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

Cryo-irradiation as a terminal method for the sterilization of drug aqueous solutions.

PubMed

Maquille, Aubert; Habib Jiwan, Jean-Louis; Tilquin, Bernard

2008-05-01

The aim of this study is to evaluate the specificities of the irradiation of drugs in frozen aqueous solution. The structures of the degradation products were determined to gain insight into the radiolysis mechanisms occurring in frozen aqueous solutions. Metoclopramide hydrochloride and metoprolol tartrate were chosen as models. The frozen solutions were irradiated at dry ice temperature by high energy electrons at various doses. The drug purity (chemical potency) and the radiolysis products were quantified by HPLC-DAD. Characterization of the degradation products was performed by LC-APCI-MS-MS. The structures of the radiolysis products detected in irradiated frozen aqueous solutions were compared to those detected in solid-state and aqueous solutions (previous studies). For both metoclopramide and metoprolol, solute loss upon irradiation of frozen aqueous solutions was negligible. Five radiolysis products present in traces were identified in irradiated metoclopramide frozen solutions. Three of them were previously identified in solid-state irradiated metoclopramide crystals. The two others were formed following reactions with the hydroxyl radical (indirect effect). Only one fragmentation product was observed in irradiated metoprolol frozen solutions. For both drugs, radiosterilization of frozen solutions, even at high doses (25 kGy), was found to be possible.

Effects of enzymes to improve sensory quality of frozen dough bread and analysis on its mechanism.

PubMed

Wang, Xuan; Pei, Dudu; Teng, Yuefei; Liang, Jianfen

2018-01-01

Baking quality of frozen dough is negatively affected by dough weakening and by a reduction in both yeast viability and activity during freezing and frozen storage. The objective of this study was to investigate effects of different enzymes, such as α-amylase, xylanase, celluase, glucose oxidase, and lipase on the texture and sensory quality of bread after frozen storage, as well as on dough properties, in terms of fermentation characteristics, freezable water contents and microstructure. Except for α-amylase, other enzymes improved the bread sensory quality and got higher overall acceptability, especially xylanase. Dough fermentative behavior showed that the maximum heights of frozen dough were increased by 33.2, 19.7 and 7.4%, respectively with xylanase, cellulase and lipase. Cellulase lowered gas holding ability of dough. Thermodynamic properties indicated that addition of enzyme decreased the freezable water contents in frozen dough. Scanning electronic microscopy revealed that freezing and frozen storage disrupted dough gluten network causing separation of starch granules from the gluten matrix. Inclusion of cellulase, xylanase and lipase made the frozen dough having a more continuous gluten network and smoother surface, and glucose oxidase increased the stability of the gluten work.

An IR Sensor Based Smart System to Approximate Core Body Temperature.

PubMed

Ray, Partha Pratim

2017-08-01

Herein demonstrated experiment studies two methods, namely convection and body resistance, to approximate human core body temperature. The proposed system is highly energy efficient that consumes only 165 mW power and runs on 5 VDC source. The implemented solution employs an IR thermographic sensor of industry grade along with AT Mega 328 breakout board. Ordinarily, the IR sensor is placed 1.5-30 cm away from human forehead (i.e., non-invasive) and measured the raw data in terms of skin and ambient temperature which is then converted using appropriate approximation formula to find out core body temperature. The raw data is plotted, visualized, and stored instantaneously in a local machine by means of two tools such as Makerplot, and JAVA-JAR. The test is performed when human object is in complete rest and after 10 min of walk. Achieved results are compared with the CoreTemp CM-210 sensor (by Terumo, Japan) which is calculated to be 0.7 °F different from the average value of BCT, obtained by the proposed IR sensor system. Upon a slight modification, the presented model can be connected with a remotely placed Internet of Things cloud service, which may be useful to inform and predict the user's core body temperature through a probabilistic view. It is also comprehended that such system can be useful as wearable device to be worn on at the hat attachable way.

Effect of sugar and acid on the acceptability of frozen yogurt to a student population.

PubMed

Guinard, J X; Little, C; Marty, C; Palchak, T R

1994-05-01

One hundred and forty-one college students tasted and rated on a nine-point hedonic scale their degree of liking for nine samples of vanilla frozen yogurt varying in sugar and lactic acid. Subjects were also asked to complete a questionnaire about consumption of frozen yogurt and other dairy products. Degree of liking differed significantly among samples, and the samples best liked were those with the lowest acidity, .23 to .29%, independent of sugar concentration. Degree of liking of frozen yogurt failed to correlate with dairy product consumption or hunger at the time of testing. No significant difference existed between male and female students for overall degree of liking of frozen yogurt or overall dairy product intake, yet the questionnaire revealed a significantly higher consumption of frozen yogurt among female students. The results of this study suggest that, for the student population tested, frozen yogurt should combine the sensory properties of ice cream (low acidity) with the nutritional properties of yogurt (low fat, active enzyme culture).

Intraoperative consultation of central nervous system lesions. Frozen section, cytology or both?

PubMed

Sharifabadi, Ali Haidari; Haeri, Hayedeh; Zeinalizadeh, Mehdi; Zargari, Neda; Razavi, Amirnader Emami; Shahbazi, Nargess; Tahvildari, Malahat; Azmoudeh-Ardalan, Farid

2016-03-01

Frozen section is the traditional method of assessing central nervous system (CNS) lesions intraoperatively. Our aim is to determine the diagnostic accuracy of frozen section and/or cytological evaluation of CNS lesions in our center. A total of 157 patients with CNS lesions underwent open surgical biopsy or excision in our center during a period of 2 years (2012-2013). All specimens were studied cytologically; of these specimens, 146 cases were also examined by frozen section. Cytology and frozen section slides were studied separately by two general pathologists who were blind to final diagnoses. The final diagnoses were based on permanent sections and IHC studies. The accuracy rates of frozen section analysis and cytological evaluation were 87% and 86%, respectively. If the two methods were considered together, the accuracy rate improved to about 95%. Cytological evaluation is an acceptable alternative to frozen section analysis and also a great supplement to the diagnosis of CNS lesions. Copyright © 2015 Elsevier GmbH. All rights reserved.

In vitro fertilization and sperm cryopreservation in the black-footed cat (Felis nigripes) and sand cat (Felis margarita).

PubMed

Herrick, J R; Campbell, M; Levens, G; Moore, T; Benson, K; D'Agostino, J; West, G; Okeson, D M; Coke, R; Portacio, S C; Leiske, K; Kreider, C; Polumbo, P J; Swanson, W F

2010-03-01

Studies of in vitro fertilization (IVF) and sperm cryopreservation have been conducted in several small cat species, but virtually no data exist for black-footed cats (Felis nigripes) (BFCs) or sand cats (Felis margarita) (SCs). The objectives of this study were 1) to compare in vitro motility and acrosome status of fresh and cryopreserved (frozen in pellets on dry ice or in straws in liquid nitrogen vapor) BFC and SC spermatozoa cultured in feline-optimized culture medium (FOCM) or Ham F-10, 2) to assess ovarian responsiveness in BFCs and SCs following exogenous gonadotropin treatment and laparoscopic oocyte recovery, and 3) to evaluate the fertility of fresh and frozen-thawed spermatozoa from both species using homologous and heterologous (domestic cat oocytes) IVF in the two culture media. Motility and acrosomal integrity of fresh and frozen-thawed spermatozoa from BFCs and SCs were similar (P > 0.05) in both media during 6 h of culture. Although effects were more pronounced in SCs, cryopreservation in straws was superior (P < 0.05) to cryopreservation in pellets for both species. Gonadotropin stimulation produced approximately 16 ovarian follicles per female, and >80% of recovered oocytes were of optimal (grade 1) quality. The BFC and SC spermatozoa fertilized 60.0%-79.4% of homologous and 37.7%-42.7% of heterologous oocytes in both culture media, with increased (P < 0.05) cleavage of homologous (SC) and heterologous (BFC and SC) oocytes in FOCM. These results provide the first information to date on the gamete biology of two imperiled cat species and further our capacity to apply reproductive technologies for their conservation.

Cooking can decrease mercury contamination of a mushroom meal: Cantharellus cibarius and Amanita fulva.

PubMed

Falandysz, Jerzy; Drewnowska, Małgorzata

2017-05-01

Mushrooms (Cantharellus cibarius and Amanita fulva) were blanched (parboiled) and pickled using different treatment conditions with the aim of carrying out the study into effect on removal of toxic mercury (Hg) accumulated in flesh. Blanching of fresh sliced C. cibarius caused leaching of Hg by approximately 15%, while loss of up to 35% was observed for sliced, deep-frozen fruit bodies. The rate of Hg leaching from the C. cibarius in practice was the same when blanched for 5 or 15 min irrespective of potable or deionized water used. Pickling of blanched C. cibarius with a diluted vinegar marinade had only a minor, if any, effect on removal of Hg and was without effect on blanched caps of A. fulva. Mercury was better extracted by boiling water from the fresh caps of A. fulva (56 ± 2% of the initial level in fresh caps) than from the fresh or frozen fruit bodies of C. cibarius. Total leaching rate of Hg from a pickled C. cibarius when fresh fruit bodies were processed was between 15 ± 5 and 37 ± 7% (median range 13-34%), and when deep-frozen fruit bodies were processed, it was between 37 ± 7 and 39 ± 8% (median range 34-39%). Pickling of the caps of A. fulva with diluted vinegar did not increase leaching of Hg. Blanching of mushrooms before future culinary use is a simple procedure recommended in reduction of contamination with Hg of cooked mushroom meal. Pickling had little if any effect on further removal of Hg from the initially blanched mushrooms.

Effects of lactic acid and commercial chilling processes on survival of Salmonella, Yersinia enterocolitica, and Campylobacter coli in pork variety meats.

PubMed

King, Amanda M; Miller, Rhonda K; Castillo, Alejandro; Griffin, Davey B; Hardin, Margaret D

2012-09-01

Current industry chilling practices with and without the application of 2% L-lactic acid were compared for their effectiveness at reducing levels of Salmonella, Yersinia enterocolitica, and Campylobacter coli on pork variety meats. Pork variety meats (livers, intestines, hearts, and stomachs) were inoculated individually with one of the three pathogens and subjected to five different treatment combinations that included one or more of the following: water wash (25°C), lactic acid spray (2%, 40 to 50°C), chilling (4°C), and freezing (-15°C). Samples were analyzed before treatment, after each treatment step, and after 2, 4, and 6 months of frozen storage. Results showed that when a lactic acid spray was used in combination with water spray, immediate reductions were approximately 0.5 log CFU per sample of Salmonella, 0.8 log CFU per sample of Y. enterocolitica, and 1.1 log CFU per sample of C. coli. Chilling, both alone and in combination with spray treatments, had little effect on pathogens, while freezing resulted in additional 0.5-log CFU per sample reductions in levels of Salmonella and Y. enterocolitica, and an additional 1.0-log CFU per sample reduction in levels of C. coli. While reductions of at least 1 log CFU per sample were observed on variety meats treated with only a water wash and subsequently frozen, samples treated with lactic acid had greater additional reductions than those treated with only a water spray throughout frozen storage. The results of this study suggest that the use of lactic acid as a decontamination intervention, when used in combination with good manufacturing practices during processing, causes significant reductions in levels of Salmonella, Y. enterocolitica, and C. coli on pork variety meats.

6. DETAIL OF MASONRY ON SOUTHWEST WING WALL. MASONRY ON ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. DETAIL OF MASONRY ON SOUTHWEST WING WALL. MASONRY ON WING WALLS IS LAID IN A RANDOM RUBBLE PATTERN. - Core Creek County Bridge, Spanning Core Creek, approximately 1 mile South of State Route 332 (Newtown Bypass), Newtown, Bucks County, PA

3. VIEW OF DOWNSTREAM ARCHES. MASONRY ABOVE ARCHES IN THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. VIEW OF DOWNSTREAM ARCHES. MASONRY ABOVE ARCHES IN THE SPANDREL WALL IS LAID IN A SEMI-COURSED RUBBLE PATTERN. - Core Creek County Bridge, Spanning Core Creek, approximately 1 mile South of State Route 332 (Newtown Bypass), Newtown, Bucks County, PA

5. DETAIL OF SOUTHERN ARCH. PIER AND ABUTMENTS HAVE BEEN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. DETAIL OF SOUTHERN ARCH. PIER AND ABUTMENTS HAVE BEEN REINFORCED WITH CONCRETE. INTRADOS HAS BEEN PARGED WITH MORTAR. - Core Creek County Bridge, Spanning Core Creek, approximately 1 mile South of State Route 332 (Newtown Bypass), Newtown, Bucks County, PA

Investigation of geomagnetic field forecasting and fluid dynamics of the core

NASA Technical Reports Server (NTRS)

Benton, E. R. (Principal Investigator)

1981-01-01

The magnetic determination of the depth of the core-mantle boundary using MAGSAT data is discussed. Refinements to the approach of using the pole-strength of Earth to evaluate the radius of the Earth's core-mantle boundary are reported. The downward extrapolation through the electrically conducting mantle was reviewed. Estimates of an upper bound for the time required for Earth's liquid core to overturn completely are presented. High order analytic approximations to the unsigned magnetic flux crossing the Earth's surface are also presented.

Optimization of viral resuspension methods for carbon-rich soils along a permafrost thaw gradient

DOE PAGES

Trubl, Gareth; Solonenko, Natalie; Chittick, Lauren; ...

2016-05-17

Permafrost stores approximately 50% of global soil carbon (C) in a frozen form; it is thawing rapidly under climate change, and little is known about viral communities in these soils or their roles in C cycling. In permafrost soils, microorganisms contribute significantly to C cycling, and characterizing them has recently been shown to improve prediction of ecosystem function. In other ecosystems, viruses have broad ecosystem and community impacts ranging from host cell mortality and organic matter cycling to horizontal gene transfer and reprogramming of core microbial metabolisms. Here we developed an optimized protocol to extract viruses from three types ofmore » high organic-matter peatland soils across a permafrost thaw gradient (palsa, moss-dominated bog, and sedge-dominated fen). Three separate experiments were used to evaluate the impact of chemical buffers, physical dispersion, storage conditions, and concentration and purification methods on viral yields. The most successful protocol, amended potassium citrate buffer with bead-beating or vortexing and BSA, yielded on average as much as 2-fold more virus-like particles (VLPs) g –1of soil than other methods tested. All method combinations yielded VLPs g –1of soil on the 10 8order of magnitude across all three soil types. The different storage and concentration methods did not yield significantly more VLPs g –1of soil among the soil types. In conclusion, this research provides much-needed guidelines for resuspending viruses from soils, specifically carbon-rich soils, paving the way for incorporating viruses into soil ecology studies.« less

Optimization of viral resuspension methods for carbon-rich soils along a permafrost thaw gradient

DOE Office of Scientific and Technical Information (OSTI.GOV)

Trubl, Gareth; Solonenko, Natalie; Chittick, Lauren

Permafrost stores approximately 50% of global soil carbon (C) in a frozen form; it is thawing rapidly under climate change, and little is known about viral communities in these soils or their roles in C cycling. In permafrost soils, microorganisms contribute significantly to C cycling, and characterizing them has recently been shown to improve prediction of ecosystem function. In other ecosystems, viruses have broad ecosystem and community impacts ranging from host cell mortality and organic matter cycling to horizontal gene transfer and reprogramming of core microbial metabolisms. Here we developed an optimized protocol to extract viruses from three types ofmore » high organic-matter peatland soils across a permafrost thaw gradient (palsa, moss-dominated bog, and sedge-dominated fen). Three separate experiments were used to evaluate the impact of chemical buffers, physical dispersion, storage conditions, and concentration and purification methods on viral yields. The most successful protocol, amended potassium citrate buffer with bead-beating or vortexing and BSA, yielded on average as much as 2-fold more virus-like particles (VLPs) g –1of soil than other methods tested. All method combinations yielded VLPs g –1of soil on the 10 8order of magnitude across all three soil types. The different storage and concentration methods did not yield significantly more VLPs g –1of soil among the soil types. In conclusion, this research provides much-needed guidelines for resuspending viruses from soils, specifically carbon-rich soils, paving the way for incorporating viruses into soil ecology studies.« less

Improved Numerical Calculation of the Single-Mode-No-Core-Single-Mode Fiber Structure Using the Fields Far from Cutoff Approximation

PubMed Central

Yang, Xianchao; Xu, Degang; Rong, Feng; Zhao, Junfa; Yao, Jianquan

2017-01-01

Multimode interferometers based on the single-mode-no-core-single-mode fiber (SNCS) structure have been widely investigated as functional devices and sensors. However, the theoretical support for the sensing mechanism is still imperfect, especially for the cladding refractive index response. In this paper, a modified model of no-core fiber (NCF) based on far from cut-off approximation is proposed to investigate the spectrum characteristic and sensing mechanism of the SNCS structure. Guided-mode propagation analysis (MPA) is used to analyze the self-image effect and spectrum response to the cladding refractive index and temperature. Verified by experiments, the performance of the SNCS structure can be estimated specifically and easily by the proposed method. PMID:28961174

Combining frozen-density embedding with the conductor-like screening model using Lagrangian techniques for response properties.

PubMed

Schieschke, Nils; Di Remigio, Roberto; Frediani, Luca; Heuser, Johannes; Höfener, Sebastian

2017-07-15

We present the explicit derivation of an approach to the multiscale description of molecules in complex environments that combines frozen-density embedding (FDE) with continuum solvation models, in particular the conductor-like screening model (COSMO). FDE provides an explicit atomistic description of molecule-environment interactions at reduced computational cost, while the outer continuum layer accounts for the effect of long-range isotropic electrostatic interactions. Our treatment is based on a variational Lagrangian framework, enabling rigorous derivations of ground- and excited-state response properties. As an example of the flexibility of the theoretical framework, we derive and discuss FDE + COSMO analytical molecular gradients for excited states within the Tamm-Dancoff approximation (TDA) and for ground states within second-order Møller-Plesset perturbation theory (MP2) and a second-order approximate coupled cluster with singles and doubles (CC2). It is shown how this method can be used to describe vertical electronic excitation (VEE) energies and Stokes shifts for uracil in water and carbostyril in dimethyl sulfoxide (DMSO), respectively. In addition, VEEs for some simplified protein models are computed, illustrating the performance of this method when applied to larger systems. The interaction terms between the FDE subsystem densities and the continuum can influence excitation energies up to 0.3 eV and, thus, cannot be neglected for general applications. We find that the net influence of the continuum in presence of the first FDE shell on the excitation energy amounts to about 0.05 eV for the cases investigated. The present work is an important step toward rigorously derived ab initio multilayer and multiscale modeling approaches. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2010 CFR

2010-04-01

... fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly... frozen by a freezing system such as blast-freezing (sub-zero Fahrenheit temperature with fast moving air...

Recommendations for Improving the Food Service Operations at MCB Camp Pendleton, CA MCAS El Toro and MCAS(H) Santa Ana, CA, MCB Camp Lejeune, NC, and MCDEC, Quantico, VA

DTIC Science & Technology

1979-12-01

Green Beans Nicoise Egg Plant Parmesan or Zucchini Parmesan Club Spinach Dinner: Cheese Ravioli (frozen) rotate or Beef Ravioli (frozen) Cheese...Manicotti (frozen) rotate or Beef Manicotti (frozen) Meat Sauce (for ravioli and manicotti) Spaghetti w/ Meatballs Assorted Pizzas Baked Lasagna Veal Parmesan

Effects of regional climate changes on the planktonic ecosystem and water environment in the frozen Notoro Lagoon, northern Japan.

PubMed

Katsuki, Kota; Seto, Koji; Noguchi, Takuro; Sonoda, Takeshi; Kim, JuYong

2012-10-01

Diatom fossils from core sediments and living diatoms from water samples of Notoro Lagoon in northern Japan were examined to evaluate natural climate effects on lagoon environmental changes. In 1974, the artificial inlet was excavated. Immediately after, the anoxic bottom water in Notoro Lagoon began to disappear due to an increasing water exchange rate. However, chemical oxygen demand (COD) in the bottom water of Notoro Lagoon gradually increased, with fluctuations, during the last 30 years. In addition, the dominant diatom assemblages in Notoro Lagoon shifted to ice-related and spring bloom taxa after the excavation. The dominant taxa of each year in the sediment core were also strongly related to the timing of lagoon ice melting. This is because the COD in Notoro Lagoon was affected by the deposited volume of blooming diatoms, which was controlled by the duration of ice cover and the timing of ice discharge to the Okhotsk Sea likely due to an air pressure pattern change over the northern North Pacific like the Pacific Decadal Oscillation (PDO). Copyright © 2012 Elsevier Ltd. All rights reserved.

Core Composition and the Magnetic Field of Mercury

NASA Astrophysics Data System (ADS)

Spohn, T.; Breuer, D.

2005-05-01

The density of Mercury suggests a core of approximately 1800 km radius and a mantle of approximately 600 km thickness. Convection in the mantle is often claimed to be capable of freezing the core over the lifetime of the solar system if the core is nearly pure iron. The thermal history calculations of Stevenson et al. (1983) and Schubert et al. (1988) suggest that about 5 weight-% sulphur are required to lower the core liquidus sufficiently to prevent complete freezing of the core and maintain a significant fluid outer core shell. Other candidates for a light alloying element require similarly large concentrations. The requirement of a significant concentration of volatile elements in the core is likely to be at variance with cosmochemical arguments for a mostly refractory, volatile poor composition of the planet. We have re-addressed the question of the freezing of Mercury's core using parameterized convection models based on the stagnant lid theory of planetary mantle convection. We have compared these results to earlier calculations (Conzelmann and Spohn, 1999) of Hermian mantle convection using a finite-amplitude convection code. We find consistently that the stagnant lid tends to thermally insulate the deep interior and we find mantle and core temperatures significantly larger than those calculated by Stevenson et al. (1983) and Schubert et al. (1988). As a consequence we find fluid outer core shells for reasonable mantle rheology parameters even for compositions with as little as 0.1 weight-% sulphur. Stevenson, D.J., T. Spohn, and G. Schubert. Icarus, 54, 466, 1983. Schubert, G. M.N. Ross, D.J. Stevenson, and T. Spohn, in Mercury, F. Vilas, C.R. Chapman and M.S. Matthews, eds., p.429, 1988. Conzelmann, V. and T. Spohn, Bull. Am. Astr. Soc., 31, 1102, 1999.

7 CFR 58.327 - Frozen cream.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Quality Specifications for Raw Material § 58.327 Frozen cream. To produce frozen cream eligible for official certification, the quality of...

7 CFR 58.327 - Frozen cream.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Quality Specifications for Raw Material § 58.327 Frozen cream. To produce frozen cream eligible for official certification, the quality of...

Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae.

PubMed

Teets, Nicholas M; Kawarasaki, Yuta; Lee, Richard E; Denlinger, David L

2011-03-01

In this study, we examined the effects of repeated cold exposure (RCE) on the survival, energy content and stress protein expression of larvae of the Antarctic midge, Belgica antarctica (Diptera: Chironomidae). Additionally, we compared results between larvae that were frozen at -5°C in the presence of water during RCE and those that were supercooled at -5°C in a dry environment. Although >95% of larvae survived a single 12 h bout of freezing at -5°C, after five cycles of RCE survival of frozen larvae dropped below 70%. Meanwhile, the survival of control and supercooled larvae was unchanged, remaining around 90% for the duration of the study. At the tissue level, frozen larvae had higher rates of cell mortality in the midgut than control and supercooled larvae. Furthermore, larvae that were frozen during RCE experienced a dramatic reduction in energy reserves; after five cycles, frozen larvae had 25% less lipid, 30% less glycogen and nearly 40% less trehalose than supercooled larvae. Finally, larvae that were frozen during RCE had higher expression of hsp70 than those that were supercooled, indicating a higher degree of protein damage in the frozen group. Results were similar between larvae that had accumulated 60 h of freezing at -5°C over five cycles of RCE and those that were frozen continuously for 60 h, suggesting that the total time spent frozen determines the physiological response. Our results suggest that it is preferable, both from a survival and energetic standpoint, for larvae to seek dry microhabitats where they can avoid inoculative freezing and remain unfrozen during RCE.

A simple temperature-based method to estimate heterogeneous frozen ground within a distributed watershed model

NASA Astrophysics Data System (ADS)

Follum, Michael L.; Niemann, Jeffrey D.; Parno, Julie T.; Downer, Charles W.

2018-05-01

Frozen ground can be important to flood production and is often heterogeneous within a watershed due to spatial variations in the available energy, insulation by snowpack and ground cover, and the thermal and moisture properties of the soil. The widely used continuous frozen ground index (CFGI) model is a degree-day approach and identifies frozen ground using a simple frost index, which varies mainly with elevation through an elevation-temperature relationship. Similarly, snow depth and its insulating effect are also estimated based on elevation. The objective of this paper is to develop a model for frozen ground that (1) captures the spatial variations of frozen ground within a watershed, (2) allows the frozen ground model to be incorporated into a variety of watershed models, and (3) allows application in data sparse environments. To do this, we modify the existing CFGI method within the gridded surface subsurface hydrologic analysis watershed model. Among the modifications, the snowpack and frost indices are simulated by replacing air temperature (a surrogate for the available energy) with a radiation-derived temperature that aims to better represent spatial variations in available energy. Ground cover is also included as an additional insulator of the soil. Furthermore, the modified Berggren equation, which accounts for soil thermal conductivity and soil moisture, is used to convert the frost index into frost depth. The modified CFGI model is tested by application at six test sites within the Sleepers River experimental watershed in Vermont. Compared to the CFGI model, the modified CFGI model more accurately captures the variations in frozen ground between the sites, inter-annual variations in frozen ground depths at a given site, and the occurrence of frozen ground.

The outcome of manipulation under general anesthesia for the management of frozen shoulder in patients with diabetes mellitus.

PubMed

Jenkins, Emily F; Thomas, William J C; Corcoran, John P; Kirubanandan, Ravisankar; Beynon, Celia R; Sayers, Adrian E; Woods, David A

2012-11-01

Frozen shoulder has a greater incidence, more severe course, and resistance to treatment in patients with diabetes mellitus compared with the general population. We hypothesized that diabetic patients with frozen shoulder undergoing treatment with manipulation under general anaesthesia (MUA) would have the same outcome as patients without diabetes. We retrospectively analyzed data collected during a 10-year period of referrals for frozen shoulder. In all cases, a standardized MUA protocol was followed once the diagnosis of frozen shoulder in the frozen phase was made; this included an early repeat MUA in individuals with recurrence. We compared outcomes for patients documented as having diabetes with a nondiabetic control group and assessed the effect of insulin dependence and frozen shoulder etiology within the diabetic group. Of a consecutive series of 315 frozen shoulders, 36 patients (39 shoulders) were included in the diabetic group, with 256 patients (274 shoulders) as controls. There was a significant improvement in range of movement and Oxford Shoulder Score (P all <.001), with no difference between diabetic and control groups at early or late follow-up (mean, 41 months). A repeat procedure was required in 36% of diabetic patients compared with 15% of control patients. Recurrence in the diabetic group was influenced by etiology (47% of primary vs 0% of secondary frozen shoulders) and insulin requirement (39% insulin-dependent vs. 31% non-insulin-dependent). We provide a strategy for the management of diabetic frozen shoulders using MUA and estimates of success and recurrence rates that may be useful when informing consent. Copyright © 2012 Journal of Shoulder and Elbow Surgery Board of Trustees. All rights reserved.

Remote sensing of frozen lakes on the North Slope of Alaska

USGS Publications Warehouse

French, N.; Savage, S.; Shuchman, R.; Edson, R.; Payne, J.; Josberger, E.

2004-01-01

We used synthetic aperture radar (SAR) images from the ERS-2 remote sensing satellite to map the freeze condition of lakes on Alaska's North Slope, the geographic region to the north of the Brooks Range. An mage from March 1997, to coincide with the period of maximum freeze depth, was used for the frozen lake mapping. Emphasis was placed on distinguishing between lakes frozen to the lakebed and lakes with some portion unfrozen to the bed (a binary classification). The result of the analysis is a map identifying lakes as frozen to the lakebed and lakes not frozen to the lakebed. This analysis of one SAR image has shown the feasibility of a simple technique for mapping frozen lake condition for supporting decision making and understanding impacts of climate change on the North Slope.

An approach to successful freezing of demi-embryos derived from day-7 bovine embryos.

PubMed

Niemann, H; Brem, G; Sacher, B; Smidt, D; Kräusslich, H

1986-04-01

The developmental capacity of frozen/thawed bisected embryos (n = 33) derived from day-7 bovine embryos was investigated and compared to ordinary embryos after freezing and thawing (n = 28) and to freshly bisected embryos (n = 19). The freezing and thawing protocol was identical for ordinary and demi-embryos. The percentage of intact embryos classified as excellent, good, or poor after thawing was 92.9 and 96.3% for ordinary and demi-embryos, respectively. Pregnancy rates of 53.8 (8 15 ), 46.2 (6 13 ), and 47.5% (9 19 ) were obtained when frozen/thawed ordinary embryos and frozen/thawed demi-embryos classified as excellent or good and sealed with an additional zona pellucida from hatched pig blastocysts or freshly bisected embryos were transferred. One pair of identical twins resulted from the transfer of frozen/thawed demi-embryos sealed with an additional zona pellucida. Transfer of four frozen/thawed demi-embryos without an additional zona pellucida led to one pregnancy. In contrast, demi-embryos derived from frozen/thawed ordinary embryos (n = 8) as well as frozen/thawed demi-embryos classified as poor (n = 6) did not result in any pregnancies although two halves were transferred per recipient. It is concluded that sealing the punctured zona pellucida improves the developmental capacity of frozen/thawed demi-embryos derived from day-7 bovine embryos, and freezing demi-embryos is more efficient compared to the splitting of frozen/thawed ordinary embryos.

Frozen soil parameterization in a distributed biosphere hydrological model

NASA Astrophysics Data System (ADS)

Wang, L.; Koike, T.; Yang, K.; Jin, R.; Li, H.

2010-03-01

In this study, a frozen soil parameterization has been modified and incorporated into a distributed biosphere hydrological model (WEB-DHM). The WEB-DHM with the frozen scheme was then rigorously evaluated in a small cold area, the Binngou watershed, against the in-situ observations from the WATER (Watershed Allied Telemetry Experimental Research). First, by using the original WEB-DHM without the frozen scheme, the land surface parameters and two van Genuchten parameters were optimized using the observed surface radiation fluxes and the soil moistures at upper layers (5, 10 and 20 cm depths) at the DY station in July. Second, by using the WEB-DHM with the frozen scheme, two frozen soil parameters were calibrated using the observed soil temperature at 5 cm depth at the DY station from 21 November 2007 to 20 April 2008; while the other soil hydraulic parameters were optimized by the calibration of the discharges at the basin outlet in July and August that covers the annual largest flood peak in 2008. With these calibrated parameters, the WEB-DHM with the frozen scheme was then used for a yearlong validation from 21 November 2007 to 20 November 2008. Results showed that the WEB-DHM with the frozen scheme has given much better performance than the WEB-DHM without the frozen scheme, in the simulations of soil moisture profile at the cold regions catchment and the discharges at the basin outlet in the yearlong simulation.

A COMPARISON OF EXPERIMENTS AND THREE-DIMENSIONAL ANALYSIS TECHNIQUES. PART II. UNPOISONED UNIFORM SLAB CORE WITH A PARTIALLY INSERTED HAFNIUM ROD AND A PARTIALLY INSERTED WATER GAP

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roseberry, R.J.

The experimental measurements and nuclear analysis of a uniformly loaded, unpoisoned slab core with a partially inserted hafnium rod and/or a partially inserted water gap are described. Comparisons of experimental data with calculated results of the UFO core and flux synthesis techniques are given. It is concluded that one of the flux synthesis techniques and the UFO code are able to predict flux distributions to within approximately -5% of experiment for most cases, with a maximum error of approximately -10% for a channel at the core- reflector boundary. The second synthesis technique failed to give comparable agreement with experiment evenmore » when various refinements were used, e.g. increasing the number of mesh points, performing the flux synthesis technique of iteration, and spectrum-weighting the appropriate calculated fluxes through the use of the SWAKRAUM code. These results are comparable to those reported in Part I of this study. (auth)« less

An RXTE Study of M87 and the Core of the Virgo Cluster

NASA Technical Reports Server (NTRS)

Reynolds, Christopher S.; Heinz, Sebastian; Fabian, Andrew C.; Begelman, Mitchell C.

1998-01-01

We present hard X-ray observations of the nearby radio galaxy M87 and the core of the Virgo cluster using the Rossi X-ray 7Tming Explorer. These are the first hard X-ray observations of M87 not affected by contamination from the nearby Seyfert 2 galaxy NGC 4388. Thermal emission from Virgo's intracluster medium is clearly detected and has a spectrum indicative of kT is approximately equal to 2.5 keV plasma with approximately 25% cosmic abundances. No non-thermal (power-law) emission from M87 is detected in the hard X-ray band, with fluctuations in the Cosmic X-ray Background being the limiting factor. Combining with ROSAT data, we infer that the X-ray spectrum of the M87 core and jet must be steep (Gamma (sub core) > 1.90 and Gamma (sub jet) > 1.75), and we discuss the implications of this result. In particular, these results are consistent with M87 being a mis-aligned BL-Lac object.

Development of 600 kV triple resonance pulse transformer.

PubMed

Li, Mingjia; Zhang, Faqiang; Liang, Chuan; Xu, Zhou

2015-06-01

In this paper, a triple-resonance pulse transformer based on an air-core transformer is introduced. The voltage across the high-voltage winding of the air-core transformer is significantly less than the output voltage; instead, the full output voltage appears across the tuning inductor. The maximum ratio of peak load voltage to peak transformer voltage is 2.77 in theory. By analyzing pulse transformer's lossless circuit, the analytical expression for the output voltage and the characteristic equation of the triple-resonance circuit are presented. Design method for the triple-resonance pulse transformer (iterated simulation method) is presented, and a triple-resonance pulse transformer is developed based on the existing air-core transformer. The experimental results indicate that the maximum ratio of peak voltage across the load to peak voltage across the high-voltage winding of the air-core transformer is approximately 2.0 and the peak output voltage of the triple-resonance pulse transformer is approximately 600 kV.

78 FR 43888 - Compliance Policy Guide Sec. 253.100-Use of Units of Plasma and Fresh Frozen Plasma Which Have...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-22

...] Compliance Policy Guide Sec. 253.100--Use of Units of Plasma and Fresh Frozen Plasma Which Have Been Thawed... guide (CPG) entitled ``Sec. 253.100--Use of Units of Plasma and Fresh Frozen Plasma Which Have Been... ``Sec. 253.100-- Use of Units of Plasma and Fresh Frozen Plasma Which Have Been Thawed'' on October 1...

Documentation and Validation of the Goddard Earth Observing System (GEOS) Data Assimilation System, Version 4

NASA Technical Reports Server (NTRS)

Suarez, Max J. (Editor); daSilva, Arlindo; Dee, Dick; Bloom, Stephen; Bosilovich, Michael; Pawson, Steven; Schubert, Siegfried; Wu, Man-Li; Sienkiewicz, Meta; Stajner, Ivanka

2005-01-01

This document describes the structure and validation of a frozen version of the Goddard Earth Observing System Data Assimilation System (GEOS DAS): GEOS-4.0.3. Significant features of GEOS-4 include: version 3 of the Community Climate Model (CCM3) with the addition of a finite volume dynamical core; version two of the Community Land Model (CLM2); the Physical-space Statistical Analysis System (PSAS); and an interactive retrieval system (iRET) for assimilating TOVS radiance data. Upon completion of the GEOS-4 validation in December 2003, GEOS-4 became operational on 15 January 2004. Products from GEOS-4 have been used in supporting field campaigns and for reprocessing several years of data for CERES.

Efficient two-component relativistic method for large systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nakai, Hiromi; Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555; CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012

This paper reviews a series of theoretical studies to develop efficient two-component (2c) relativistic method for large systems by the author’s group. The basic theory is the infinite-order Douglas-Kroll-Hess (IODKH) method for many-electron Dirac-Coulomb Hamiltonian. The local unitary transformation (LUT) scheme can effectively produce the 2c relativistic Hamiltonian, and the divide-and-conquer (DC) method can achieve linear-scaling of Hartree-Fock and electron correlation methods. The frozen core potential (FCP) theoretically connects model potential calculations with the all-electron ones. The accompanying coordinate expansion with a transfer recurrence relation (ACE-TRR) scheme accelerates the computations of electron repulsion integrals with high angular momenta and longmore » contractions.« less

Computer enhancement of ESR spectra of magnetite nanoparticles

NASA Astrophysics Data System (ADS)

Dobosz, B.; Krzyminiewski, R.; Koralewski, M.; Hałupka-Bryl, M.

2016-06-01

We present ESR measurements of non-interacting magnetic nanoparticle systems. Temperature and orientational dependence of ESR spectra were measured for Fe3O4 nanoparticle coated by dextran or oleic acid, frozen in different magnetic field. Several parameters describing magnetic properties such as g-factor, line width, the anisotropy constant were calculated and discussed. The ESR spectra of investigated nanoparticles were also subjected to Computer Resolution Enhancement Method (CREM). This procedure allows to separate a narrow line on the background of the broad line, which presence in this type of materials was recognized in the recent literature and have been further discussed in the paper. CREM is a valuable tool for monitoring of changes on the surface of magnetic core of nanoparticles.

Production of a Functional Frozen Yogurt Fortified with Bifidobacterium spp.

PubMed

Abdelazez, Amro; Muhammad, Zafarullah; Zhang, Qiu-Xue; Zhu, Zong-Tao; Abdelmotaal, Heba; Sami, Rokayya; Meng, Xiang-Chen

2017-01-01

Frozen dairy products have characteristics of both yogurt and ice cream and could be the persuasive carriers of probiotics. Functions of the frozen yogurt containing viable bifidobacterial cells are recognized and favored by the people of all ages. We developed a kind of yogurt supplemented by Bifidobacterium species. Firstly, five strains of Bifidobacterium spp. ( Bifidobacterium bifidum ATCC 11547, Bifidobacterium longum ATCC 11549, Bifidobacterium infantis ATCC 11551, Bifidobacterium adolescentis ATCC 11550, and Bifidobacterium breve ATCC 11548) were evaluated based on the feasibility criteria of probiotics, comprising acid production, bile tolerance, and adhesion to epithelial cells. Formerly, we combined the optimum strains with yogurt culture ( Lactobacillus delbrueckii subsp. bulgaricus EMCC 11102 and Streptococcus salivarius subsp. thermophilus EMCC 11044) for producing frozen yogurt. Finally, physiochemical properties and sensory evaluation of the frozen yogurt were investigated during storage of 60 days at -18°C. Results directed that Bifidobacterium adolescentis ATCC 11550 and Bifidobacterium infantis ATCC 11551 could be utilized with yogurt culture for producing frozen yogurt. Moreover, the frozen yogurt fermented by two bifidobacterial strains and yogurt culture gained the high evaluation in the physiochemical properties and sensory evaluation. In summary, our results revealed that there was no significant difference between frozen yogurt fermented by Bifidobacterium spp. and yogurt culture and that fermented by yogurt culture only.

A retrospective study of artificial insemination of 251 mares using chilled and fixed time frozen-thawed semen.

PubMed

Crowe, C A M; Ravenhill, P J; Hepburn, R J; Shepherd, C H

2008-09-01

Historically, artificial insemination (AI) using frozen semen has been perceived to have poorer success rates and be more labour intensive than using chilled semen. A retrospective study was therefore conducted to compare the conception rate achieved by AI between chilled and frozen semen, using fixed time insemination protocols over 2 breeding seasons. Artificial insemination using chilled semen produces a higher conception rate than that achieved with frozen semen. Mares (n = 251) were inseminated with either chilled (n = 112) or frozen (n = 139) semen in the 2006 and 2007 northern hemisphere breeding season. Per rectum ultrasonography of the mare's reproductive tract determined the timing of insemination, and deslorelin acetate was used to induce ovulation. Chilled semen insemination was performed using a single preovulatory dose delivered into the uterine body. Frozen semen was administered as 2 doses (pre- and post ovulation) using a deep uterine insemination technique. Pregnancy was detected ultrasonographically at 15 days post insemination. Conception rates were compared using a Chi-squared test. Insemination with frozen semen produced a significantly (P = 0.022) higher seasonal conception rate (82.0%) than that achieved with chilled semen (69.6%). Insemination with frozen semen can achieve conception rates equal to those with chilled semen, enabling the mare owner a greater selection of stallions.

Presentation of frozen shoulder among diabetic and non-diabetic patients.

PubMed

Uddin, Mohammad Moin; Khan, Aminuddin A; Haig, Andrew J; Uddin, Mohammad Kafil

2014-12-01

The literature is inconsistent regarding the level of pain and disability in frozen shoulder patients with or without diabetes mellitus. The aim of this study is to evaluate some demographic features of frozen shoulder patients and to look into the disparity of information by comparing the level of pain and disability due to frozen shoulder between diabetic and non-diabetic people. This is a prospective comparative study. People with frozen shoulder attending an outpatient department were selected by consecutive sampling. Disability levels were assessed by the Shoulder Pain & Disability Index (SPADI). Means of pain and disability scores were compared using unpaired t-test. Among 140 persons with shoulder pain 99 (71.4%) had frozen shoulder. From the participating 40 frozen shoulder patients, 26 (65%) were males and 14 (35%) were females. Seventeen participants (42.5%) were diabetic, two (5%) had impaired glucose tolerance and 21 (52.5%) patients were non-diabetic. Mean disability scores (SPADI) were 51 ± 15.5 in diabetic and 57 ± 16 in non-diabetic persons. The differences in pain and disability level were not statistically significance (respectively, p = 0.24 and p = 0.13 at 95% confidence interval). No difference was found in level of pain and disability level between frozen shoulder patients with and without diabetes.

Texture and microstructure properties of frozen chicken breasts pretreated with salt and phosphate solutions.

PubMed

Yoon, K S

2002-12-01

This study investigated the effects of 10% NaCl, trisodium phosphate (TSP), sodium tripolyphosphate (STPP), and tetrapotassium pyrophosphate (TKPP) treatments on textural and microstructural properties of chicken breasts during 10 mo of frozen storage at -20 C. Fresh chicken breasts were treated for 10 min with 10% NaCl and various phosphate solutions, including TSP, STPP, and TKPP, and stored in a -20 C freezer for 10 mo. Frozen chicken breasts were completely thawed at 4 C and oven-baked at 177 C for 20 min. Shear force, drip loss, and cooking loss were measured. In addition, ice crystal formation and structure changes of frozen chicken breasts during storage were evaluated using transmission electron microscopy (TEM). Treating chicken breasts with 10% TSP and STPP solution significantly reduced drip and cooking losses as well as minimized ice crystal formation and freeze-induced shrinkage of myofibrils. No significant texture toughening was observed in frozen chicken breasts regardless of treatments. These results suggest that the perceived quality losses of frozen chicken breast were not associated with texture toughening. The water-binding ability of chicken meat was the most important factor in maintaining the quality of chicken breast during extended frozen storage, which can be accomplished by treating chicken breasts with 10% TSP and STPP solutions before frozen storage.

Production of a Functional Frozen Yogurt Fortified with Bifidobacterium spp.

PubMed Central

Muhammad, Zafarullah; Zhang, Qiu-Xue; Zhu, Zong-Tao

2017-01-01

Frozen dairy products have characteristics of both yogurt and ice cream and could be the persuasive carriers of probiotics. Functions of the frozen yogurt containing viable bifidobacterial cells are recognized and favored by the people of all ages. We developed a kind of yogurt supplemented by Bifidobacterium species. Firstly, five strains of Bifidobacterium spp. (Bifidobacterium bifidum ATCC 11547, Bifidobacterium longum ATCC 11549, Bifidobacterium infantis ATCC 11551, Bifidobacterium adolescentis ATCC 11550, and Bifidobacterium breve ATCC 11548) were evaluated based on the feasibility criteria of probiotics, comprising acid production, bile tolerance, and adhesion to epithelial cells. Formerly, we combined the optimum strains with yogurt culture (Lactobacillus delbrueckii subsp. bulgaricus EMCC 11102 and Streptococcus salivarius subsp. thermophilus EMCC 11044) for producing frozen yogurt. Finally, physiochemical properties and sensory evaluation of the frozen yogurt were investigated during storage of 60 days at −18°C. Results directed that Bifidobacterium adolescentis ATCC 11550 and Bifidobacterium infantis ATCC 11551 could be utilized with yogurt culture for producing frozen yogurt. Moreover, the frozen yogurt fermented by two bifidobacterial strains and yogurt culture gained the high evaluation in the physiochemical properties and sensory evaluation. In summary, our results revealed that there was no significant difference between frozen yogurt fermented by Bifidobacterium spp. and yogurt culture and that fermented by yogurt culture only. PMID:28691028

Contribution of Transjugular Liver Biopsy in Patients with the Clinical Presentation of Acute Liver Failure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miraglia, Roberto, E-mail: rmiraglia@ismett.edu; Luca, Angelo; Gruttadauria, Salvatore

2006-12-15

Purpose. Acute liver failure (ALF) treated with conservative therapy has a poor prognosis, although individual survival varies greatly. In these patients, the eligibility for liver transplantation must be quickly decided. The aim of this study was to assess the role of transjugular liver biopsy (TJLB) in the management of patients with the clinical presentation of ALF. Methods. Seventeen patients with the clinical presentation of ALF were referred to our institution during a 52 month period. A TJLB was performed using the Cook Quick-Core needle biopsy. Clinical data, procedural complications, and histologic findings were evaluated. Results. Causes of ALF were virusmore » hepatitis B infection in 7 patients, drug toxicity in 4, mushroom in 1, Wilson's disease in 1, and unknown origin in 4. TJLB was technically successful in all patients without procedure-related complications. Tissue specimens were satisfactory for diagnosis in all cases. In 14 of 17 patients the initial clinical diagnosis was confirmed by TJLB; in 3 patients the initial diagnosis was altered by the presence of unknown cirrhosis. Seven patients with necrosis <60% were successfully treated with medical therapy; 6 patients with submassive or massive necrosis ({>=}85%) were treated with liver transplantation. Four patients died, 3 had cirrhosis, and 1 had submassive necrosis. There was a strict statistical correlation (r = 0.972, p < 0.0001) between the amount of necrosis at the frozen section examination and the necrosis found at routine histologic examination. The average time for TJLB and frozen section examination was 80 min. Conclusion. In patients with the clinical presentation of ALF, submassive or massive liver necrosis and cirrhosis are predictors of poor prognosis. TLJB using an automated device and frozen section examination can be a quick and effective tool in clinical decision-making, especially in deciding patient selection and the best timing for liver transplantation.« less

Climate and Cryosphere (CliC) Project and its Interest in Arctic Hydrology Research

NASA Astrophysics Data System (ADS)

Yang, D.; Prowse, T. D.; Steffen, K.; Ryabinin, V.

2009-12-01

The cryosphere is an important and dynamic component of the global climate system. The global cryosphere is changing rapidly, with changes in the Polar Regions receiving particular attention during the International Polar Year 2007-2008. The Climate and Cryosphere (CliC) Project is a core project of the World Climate Research Programme (WCRP) and is co-sponsored by WCRP, SCAR (Scientific Committee for Antarctic Research) and IASC (International Committee for Antarctic Research). The principal goal of CliC is to assess and quantify the impacts that climatic variability and change have on components of the cryosphere and the consequences of these impacts for the climate system. To achieve its objectives, CliC coordinates international and regional projects, partners with other organizations in joint initiatives, and organizes panels and working groups to lead and coordinate advanced research aimed at closing identified gaps in scientific knowledge about climate and cryosphere. The terrestrial cryosphere includes land areas where snow cover, lake- and river-ice, glaciers and ice caps, permafrost and seasonally frozen ground and solid precipitation occur. The main task of this theme is to improve estimates and quantify the uncertainty of water balance and related energy flux components in cold climate regions. This includes precipitation (both solid and liquid) distribution, properties of snow, snow melt, evapotranspiration, sublimation, water movement through frozen and unfrozen ground, water storage in watersheds, river- and lake-ice properties and processes, and river runoff. The focus of this theme includes two specific issues: the role of permafrost and frozen ground in the carbon balance, and precipitation in cold climates. Hydrological studies of cold regions will provide a key contribution to the new theme crosscut, which focuses on the cryospheric input to the freshwater balance of the Arctic. This presentation will provide an overview and update of recent developments of cold region hydrometeorology research activities and future challenges in arctic hydrology and climate change investigations.

The single scattering properties of soot aggregates with concentric core-shell spherical monomers

NASA Astrophysics Data System (ADS)

Wu, Yu; Cheng, Tianhai; Gu, Xingfa; Zheng, Lijuan; Chen, Hao; Xu, Hui

2014-03-01

Anthropogenic soot aerosols are shown as complex, fractal-like aggregated structures with high light absorption efficiency. In atmospheric environment, soot monomers may tend to acquire a weakly absorbing coating, such as an organic coating, which introduces further complexity to the optical properties of the aggregates. The single scattering properties of soot aggregates can be significantly influenced by the coated status of these kinds of aerosols. In this article, the monomers of fractal soot aggregates are modelled as semi-external mixtures (physical contact) with constant radius of soot core and variable sizes of the coating for specific soot volume fractions. The single scattering properties of these coated soot particles, such as phase function, the cross sections of extinction and absorption, single scattering albedo (SSA) and asymmetry parameter (ASY), are calculated using the numerically exact superposition T-matrix method. The random-orientation averaging results have shown that the single scattering properties of these coated soot aggregates are significantly different from the single volume-equivalent core-shell sphere approximation using the Mie theory and the homogeneous aggregates with uncoated monomers using the effective medium theory, such as Maxwell-Garnett and Bruggemann approximations, which overestimate backscattering of coated soot. It is found that the SSA and cross sections of extinction and absorption are increased for soot aggregates with thicker weakly absorbing coating on the monomers. Especially, the SSA values of these simulated aggregates with less soot core volume fractions are remarkably (~50% for core volume fraction of soot aggregates of 0.5, ~100% for a core volume fraction of 0.2, at 0.67 μm) larger than for uncoated soot particles without consideration of coating. Moreover, the cross sections of extinction and absorption are underestimated by the computation of equivalent homogeneous fractal aggregate approximation (within 5% for the T-matrix method and 10-25% for the Rayleigh-Debye-Gans approximation due to different soot volume fractions). Further understanding of the optical properties of these coated soot aggregates would be helpful for both environment monitoring and climate studies.

Sulfur Speciation in Peat: a Time-zero Signature for the " Spruce and Peatland Responses Under Climate and Environmental Change" Experiment

NASA Astrophysics Data System (ADS)

Furman, O.; Toner, B. M.; Sebestyen, S. D.; Kolka, R. K.; Nater, E. A.

2014-12-01

As part of the "Spruce and Peatland Responses Under Climate and Environmental Change" (SPRUCE) experiment, we made initial measurements of sulfur speciation in peat. These observations represent a "time-zero" relative to the intended soil warming experiment which begins in 2015. Total sulfur and sulfur speciation were measured in peat cores (solid phase) from nine plots (hollows and hummocks) to a depth of 2 m. Peat samples were packed under nitrogen and frozen in the field immediately after collection. All subsequent sample storage, handling, and processing were conducted under inert gas. Sulfur speciation was measured using bulk sulfur 1s X-ray absorption near edge structure (XANES) spectroscopy at the SXRMB instrument at the Canadian Light Source, Saskatoon, SK, Canada and at the 9-BM instrument, Advanced Photon Source, Argonne National Laboratory, IL, USA. Total sulfur concentrations ranged from 968 to 4077 mg sulfur / kg dry peat. Sulfur content increased with depth from 2 g sulfur / m2 in the 0-10 cm increment to a maximum value of 38 g sulfur / m2 in the 50-60 cm increment. These sulfur loadings produced high quality XANES spectra. The nine cores exhibited reproducible trends with depth in both total sulfur and specific sulfur species; however, variability in sulfur speciation was greatest in the top 40 cm. All sulfur detected within the peat solids was in an organic form. The most abundant sulfur species group was composed of organic mono-sulfide and thiol forms, representing approximately half of the total sulfur at all depths. Sulfonate and ester-sulfate species were 10-15 mol% of sulfur and exhibited low variability with depth. A subsurface maximum in organic di-sulfide was observed in the 20-30 cm depth increment, which is the transition zone between transiently oxidized acrotelm and permanently saturated anaerobic catotelm. Quantification of major sulfur pools is important for the SPRUCE experiment as they are likely to be indicators of changes in the oxidation-reduction (redox) status, and mercury methylation potential, of the peat in response to warming and enhanced carbon dioxide.

ROSAT X-ray sources embedded in the rho Ophiuchi cloud core

NASA Astrophysics Data System (ADS)

Casanova, Sophie; Montmerle, Thierry; Feigelson, Eric D.; Andre, Philippe

1995-02-01

We present a deep ROSAT Position Sensitive Proportional Counter (PSPC) image of the central region of the rho Oph star-forming region. The selected area, about 35 x 35 arcmins in size, is rich with dense molecular cores and young stellar objects (YSOs). Fifty-five reliable X-ray sources are detected (and up to 50 more candidates may be present) above approximately 1 keV,, doubling the number of Einstein sources in this area. These sources are cross-identified with an updated list of 88 YSOs associated with the rho Oph cloud core. A third of the reliable X-ray sources do not have optical counterparts on photographic plates. Most can be cross-identified wth Class II and Class III infrared (IR) sources, which are embedded T Tauri stars, but three reliable X-ray sources and up to seven candidate sources are tentatively identified with Class I protostars. Eighteen reliable, and up to 20 candidate, X-ray sources are probably new cloud members. The overall detection rate of the bona fide cloud population is very high (73% for the Class II and Class III objects). The spatial distribution of the X-ray sources closely follows that of the moleclar gas. The visual extinctions Av estimated from near-IR data) of the ROSAT sources can be as high as 50 or more, confirming that most are embedded in the cloud core and are presumably very young. Using bolometric luminosities Lbol estimated from J-magnitudes a tight correlation between Lx and Lbol is found, similar to that seen for older T Tauri stars in the Cha I cloud: Lx approximately 10-4 Lbol. A general relation Lxproportional to LbolLj seems to apply to all T Tauri-like YSOs. The near equality of the extintion in the IR J band and in the keV X-ray rage implies that this relation is valid for the detected fluxes as well as for the dereddened fluxes. The X-ray luminosity function of the embedded sourced in rho Oph spans a range of Lx approximately 1028.5 to approximately equal to or greater than 1031.5 ergs/s and is statistically indistinguishable from that of X-ray-detected visile T Tauri stars. We estimate a total X-ray luminosity Lx, Oph approximately equal to or greater than 6 x 10 32 ergs/s from approximately equal to 200 X-ray sources in the cloud core, down to Lbol approximately 0.1 solar luminosity or Mstar approximately 0.3 solar mass. We discuss several consequences of in situ irradiation of molecular clouds by X-rays from embedded YSOs. These X-rays must partially ionize the inner regions of circumstellar disk coronae, possibly playing an important role in coupling magnetic ionize the fields and wind or bipolar outflows. Photon-stimulated deportion of large molecules by YSO X-rays may be partly responsible for the bright 12 micrometer halos seen in some molecular clouds.

Biogenesis of the Secretory Granule: Chromogranin a Coiled-Coil Structure Results in Unusual Physical Properties And Suggests a Mechanism for Granule Core Condensation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mosley, C.A.; Taupenot, L.; Biswas, N.

2009-06-03

The secretory pro-hormone chromogranin A (CHGA) is densely packed into storage granules along with catecholamines, playing a catalytic role in granule biogenesis. 3-Dimensional structural data on CHGA are lacking. We found a superfamily structural homology for CHGA in the tropomyosin family of alpha-helical coiled-coils, even in mid-molecule regions where primary sequence identity is only modest. The assignment was confirmed by an independent algorithm, suggesting approximately 6-7 such domains spanning CHGA. We provide additional physiochemical evidence (chromatographic, spectral, microscopic) consistent with this unusual structure. Alpha-helical secondary structure (at up to approximately 45%) was confirmed by circular dichroism. CHGA molecular mass wasmore » estimated by MALDI-TOF mass spectrometry at approximately 50 kDa and by denaturing gel filtration at approximately 50-61 kDa, while its native Stokes radius was approximately 84.8 A, as compared to an expected approximately 30 A; the increase gave rise to an apparent native molecular weight of approximately 578 kDa, also consistent with the extended conformation of a coiled-coil. Small-angle X-ray scattering (SAXS) on CHGA in solution best fit an elongated cylindrical conformation in the monodisperse region with a radius of gyration of the rod cross-section (Rt) of approximately 52 A, compatible with a coiled-coil in the hydrated, aqueous state, or a multimeric coiled-coil. Electron microscopy with negative staining revealed an extended, filamentous CHGA structure with a diameter of approximately 94 +/- 4.5 A. Extended, coiled-coil conformation is likely to permit protein 'packing' in the secretory granule at approximately 50% higher density than a globular/spherical conformation. Natural allelic variation in the catestatin region was predicted to disrupt the coiled-coil. Chromaffin granule ultrastructure revealed a approximately 108 +/- 6.3 A periodicity of electron density, suggesting nucleation of a binding complex by the CHGA core. Inhibition of CHGA expression, by siRNA, disrupted regulated secretory protein traffic by approximately 65%, while targeted ablation of the CHGA gene in the mouse reduced chromaffin granule cotransmitter concentrations by approximately 40-80%. These results suggest new roles for secretory protein tertiary structure in hormone and transmitter storage, with implications for secretory cargo condensation (or dense core 'packing' structure) within the regulated pathway.« less

Impact of HFIR LEU Conversion on Beryllium Reflector Degradation Factors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ilas, Dan

2013-10-01

An assessment of the impact of low enriched uranium (LEU) conversion on the factors that may cause the degradation of the beryllium reflector is performed for the High Flux Isotope Reactor (HFIR). The computational methods, models, and tools, comparisons with previous work, along with the results obtained are documented and discussed in this report. The report documents the results for the gas and neutronic poison production, and the heating in the beryllium reflector for both the highly enriched uranium (HEU) and LEU HFIR configurations, and discusses the impact that the conversion to LEU may have on these quantities. A time-averagingmore » procedure was developed to calculate the isotopic (gas and poisons) production in reflector. The sensitivity of this approach to different approximations is gauged and documented. The results show that the gas is produced in the beryllium reflector at a total rate of 0.304 g/cycle for the HEU configuration; this rate increases by ~12% for the LEU case. The total tritium production rate in reflector is 0.098 g/cycle for the HEU core and approximately 11% higher for the LEU core. A significant increase (up to ~25%) in the neutronic poisons production in the reflector during the operation cycles is observed for the LEU core, compared to the HEU case, for regions close to the core s horizontal midplane. The poisoning level of the reflector may increase by more than two orders of magnitude during long periods of downtime. The heating rate in the reflector is estimated to be approximately 20% lower for the LEU core than for the HEU core. The decrease is due to a significantly lower contribution of the heating produced by the gamma radiation for the LEU core. Both the isotopic (gas and neutronic poisons) production and the heating rates are spatially non-uniform throughout the beryllium reflector volume. The maximum values typically occur in the removable reflector and close to the midplane.« less

Evaluation of Container Closure System Integrity for Frozen Storage Drug Products.

PubMed

Nieto, Alejandra; Roehl, Holger; Brown, Helen; Nikoloff, Jonas; Adler, Michael; Mahler, Hanns-Christian

2016-01-01

Sometimes, drug product for parenteral administration is stored in a frozen state (e.g., -20 °C or -80 °C), particularly during early stages of development of some biotech molecules in order to provide sufficient stability. Shipment of frozen product could potentially be performed in the frozen state, yet possibly at different temperatures, for example, using dry ice (-80 °C). Container closure systems of drug products usually consist of a glass vial, rubber stopper, and an aluminum crimped cap. In the frozen state, the glass transition temperature (Tg) of commonly used rubber stoppers is between -55 and -65 °C. Below their Tg, rubber stoppers are known to lose their elastic properties and become brittle, and thus potentially fail to maintain container closure integrity in the frozen state. Leaks during frozen temperature storage and transportation are likely to be transient, yet, can possibly risk container closure integrity and lead to microbial contamination. After thawing, the rubber stopper is supposed to re-seal the container closure system. Given the transient nature of the possible impact on container closure integrity in the frozen state, typical container closure integrity testing methods (used at room temperature conditions) are unable to evaluate and thus confirm container closure integrity in the frozen state. Here we present the development of a novel method (thermal physical container closure integrity) for direct assessment of container closure integrity by a physical method (physical container closure integrity) at frozen conditions, using a modified He leakage test. In this study, different container closure systems were evaluated with regard to physical container closure integrity in the frozen state to assess the suitability of vial/stopper combinations and were compared to a gas headspace method. In summary, the thermal physical container closure integrity He leakage method was more sensitive in detecting physical container closure integrity impact than gas headspace and aided identification of an unsuitable container closure system. Sometimes, drug product for parenteral administration is stored in a frozen state (e.g., -20 °C or -80 °C), particularly during early stages of development of some biotech molecules in order to provide sufficient stability. Container closure systems for drug products usually consist of a glass vial, rubber stopper, and an aluminum crimped cap. In the frozen state, the glass transition temperature (Tg) of commonly used rubber stoppers is between -55 and -65 °C. Leaks during frozen temperature storage and transportation are likely to be transient, yet they can possibly risk container closure integrity and lead to microbial contamination and sterility breach. After thawing, the rubber stopper is expected to re-seal the container closure system. Given the transient nature of the possible impact on container closure integrity in the frozen state, typical container closure integrity testing methods (used at room temperature conditions) are unable to evaluate and thus confirm container closure integrity in the frozen state. Here we present the development of a novel method (thermal container closure integrity) for direct measurement of container closure integrity by a physical method (physical container closure integrity) at frozen conditions, using a modified He leakage test. In this study, we found that the thermal container closure integrity He leakage method was more sensitive in detecting physical container closure integrity impact than gas headspace and aided identification of an unsuitable container closure system. © PDA, Inc. 2016.

Core-core and core-valence correlation

NASA Technical Reports Server (NTRS)

Bauschlicher, Charles W., Jr.; Langhoff, Stephen R.; Taylor, Peter R.

1988-01-01

The effect of (1s) core correlation on properties and energy separations was analyzed using full configuration-interaction (FCI) calculations. The Be 1 S - 1 P, the C 3 P - 5 S and CH+ 1 Sigma + or - 1 Pi separations, and CH+ spectroscopic constants, dipole moment and 1 Sigma + - 1 Pi transition dipole moment were studied. The results of the FCI calculations are compared to those obtained using approximate methods. In addition, the generation of atomic natural orbital (ANO) basis sets, as a method for contracting a primitive basis set for both valence and core correlation, is discussed. When both core-core and core-valence correlation are included in the calculation, no suitable truncated CI approach consistently reproduces the FCI, and contraction of the basis set is very difficult. If the (nearly constant) core-core correlation is eliminated, and only the core-valence correlation is included, CASSCF/MRCI approached reproduce the FCI results and basis set contraction is significantly easier.

Hypervelocity Launching and Frozen Fuels as a Major Contribution to Spaceflight

NASA Astrophysics Data System (ADS)

Cocks, F. H.; Harman, C. M.; Klenk, P. A.; Simmons, W. N.

Acting as a virtual first stage, a hypervelocity launch together with the use of frozen hydrogen/frozen oxygen propellant, offers a Single-Stage-To-Orbit (SSTO) system that promises an enormous increase in SSTO mass-ratio. Ram acceleration provides hypervelocity (2 km/sec) to the orbital vehicle with a gas gun supplying the initial velocity required for ram operation. The vehicle itself acts as the center body of a ramjet inside a launch tube, filled with gaseous fuel and oxidizer, acting as an engine cowling. The high acceleration needed to achieve hypervelocity precludes a crew, and it would require greatly increased liquid fuel tank structural mass if a liquid propellant is used for post-launch vehicle propulsion. Solid propellants do not require as much fuel- chamber strengthening to withstand a hypervelocity launch as do liquid propellants, but traditional solid fuels have lower exhaust velocities than liquid hydrogen/liquid oxygen. The shock-stability of frozen hydrogen/frozen oxygen propellant has been experimentally demonstrated. A hypervelocity launch system using frozen hydrogen/frozen oxygen propellant would be a revolutionary new development in spaceflight.

[Influence of S-nitrosoglutathione on agglutination and nitric oxide concentration in frozen platelets].

PubMed

Wu, Tao; Liu, Jing-Han; Li, Hui; Zhou, Wu; Wang, Shu-Ying

2012-04-01

The aim of this study was to investigate the influence of S-nitrosoglutathione (GSNO) on agglutination and nitric oxide (NO) concentration in frozen platelets. The agglutination of platelets was detected by using platelet agglutination apparatus, the level of NO in platelets was detected by the nitrate enzyme reduction method. The results showed that the rates of agglutination in freeze platelets and frozen platelets treated with GSNO were (35.47 ± 2.93) and (24.43 ± 3.07), which were significantly lower than that in fresh liquid platelets (63.44 ± 2.96). The level of NO concentration in frozen platelets was (22.16 ± 6.38), which was significantly lower than that in fresh liquid platelets (31.59 ± 16.88). The level of NO concentration in frozen platelets treated with GSNO was (45.64 ± 6.31), which was significantly higher than that in fresh liquid platelets (P < 0.01). It is concluded that GSNO increases the concentration of NO in frozen platelets, inhibits platelet activation and maintains platelet function, thus GSNO can be used as a frozen protective agent.

Effect of different cooking methods on color, phytochemical concentration, and antioxidant capacity of raw and frozen brassica vegetables.

PubMed

Pellegrini, Nicoletta; Chiavaro, Emma; Gardana, Claudio; Mazzeo, Teresa; Contino, Daniele; Gallo, Monica; Riso, Patrizia; Fogliano, Vincenzo; Porrini, Marisa

2010-04-14

This study evaluated the effect of common cooking practices (i.e., boiling, microwaving, and basket and oven steaming) on the phytochemical content (carotenoids, chlorophylls, glucosinolates, polyphenols, and ascorbic acid), total antioxidant capacity (TAC), and color changes of three generally consumed Brassica vegetables analyzed fresh and frozen. Among cooking procedures, boiling determined an increase of fresh broccoli carotenoids and fresh Brussels sprout polyphenols, whereas a decrease of almost all other phytochemicals in fresh and frozen samples was observed. Steaming procedures determined a release of polyphenols in both fresh and frozen samples. Microwaving was the best cooking method for maintaining the color of both fresh and frozen vegetables and obtaining a good retention of glucosinolates. During all cooking procedures, ascorbic acid was lost in great amount from all vegetables. Chlorophylls were more stable in frozen samples than in fresh ones, even though steaming methods were able to better preserve these compounds in fresh samples than others cooking methods applied. The overall results of this study demonstrate that fresh Brassica vegetables retain phytochemicals and TAC better than frozen samples.

Benchmark gas core critical experiment.

NASA Technical Reports Server (NTRS)

Kunze, J. F.; Lofthouse, J. H.; Cooper, C. G.; Hyland, R. E.

1972-01-01

A critical experiment with spherical symmetry has been conducted on the gas core nuclear reactor concept. The nonspherical perturbations in the experiment were evaluated experimentally and produce corrections to the observed eigenvalue of approximately 1% delta k. The reactor consisted of a low density, central uranium hexafluoride gaseous core, surrounded by an annulus of void or low density hydrocarbon, which in turn was surrounded with a 97-cm-thick heavy water reflector.

210Pb and 137Cs as chronometers for salt marsh accretion in the Venice Lagoon - links to flooding frequency and climate change.

PubMed

Bellucci, L G; Frignani, M; Cochran, J K; Albertazzi, S; Zaggia, L; Cecconi, G; Hopkins, H

2007-01-01

Five salt marsh sediment cores from different parts of the Venice Lagoon were studied to determine their depositional history and its relationship with the environmental changes occurred during the past approximately 100 years. X-radiographs of the cores show no disturbance related to particle mixing. Accretion rates were calculated using a constant flux model applied to excess (210)Pb distributions in the cores. The record of (137)Cs fluxes to the sites, determined from (137)Cs profiles and the (210)Pb chronologies, shows inputs from the global fallout of (137)Cs in the late 1950s to early 1960s and the Chernobyl accident in 1986. Average accretion rates in the cores are comparable to the long-term average rate of mean sea level rise in the Venice Lagoon ( approximately 0.25 cm y(-1)) except for a core collected in a marsh presumably affected by inputs from the Dese River. Short-term variations in accretion rate are correlated with the cumulative frequency of flooding, as determined by records of Acqua Alta, in four of the five cores, suggesting that variations in the phenomena causing flooding (such as wind patterns, storm frequency and NAO) are short-term driving forces for variations in marsh accretion rate.

Dynamical cluster approximation plus semiclassical approximation study for a Mott insulator and d-wave pairing

NASA Astrophysics Data System (ADS)

Kim, SungKun; Lee, Hunpyo

2017-06-01

Via a dynamical cluster approximation with N c = 4 in combination with a semiclassical approximation (DCA+SCA), we study the doped two-dimensional Hubbard model. We obtain a plaquette antiferromagnetic (AF) Mott insulator, a plaquette AF ordered metal, a pseudogap (or d-wave superconductor) and a paramagnetic metal by tuning the doping concentration. These features are similar to the behaviors observed in copper-oxide superconductors and are in qualitative agreement with the results calculated by the cluster dynamical mean field theory with the continuous-time quantum Monte Carlo (CDMFT+CTQMC) approach. The results of our DCA+SCA differ from those of the CDMFT+CTQMC approach in that the d-wave superconducting order parameters are shown even in the high doped region, unlike the results of the CDMFT+CTQMC approach. We think that the strong plaquette AF orderings in the dynamical cluster approximation (DCA) with N c = 4 suppress superconducting states with increasing doping up to strongly doped region, because frozen dynamical fluctuations in a semiclassical approximation (SCA) approach are unable to destroy those orderings. Our calculation with short-range spatial fluctuations is initial research, because the SCA can manage long-range spatial fluctuations in feasible computational times beyond the CDMFT+CTQMC tool. We believe that our future DCA+SCA calculations should supply information on the fully momentum-resolved physical properties, which could be compared with the results measured by angle-resolved photoemission spectroscopy experiments.

Analysis of the market for a new frozen coal release device

NASA Technical Reports Server (NTRS)

Taglio, S.

1981-01-01

Conditions that contribute to coal freezing and the costs of frozen coal handling are discussed. The various methods currently used to handle frozen coal are examined and assessed relative to costs, effectiveness, and advantages and disadvantages. A NASA designed gas detonation lance for breaking the ice bonds between frozen coal nuggets is described and illustrated. Market demand for the controlled gas detonation lance is estimated to be at least 10 units.

An Evaluation of the New ’Multi-Restaurant’ Food Service System for the Marine Corps

DTIC Science & Technology

1980-07-01

variety: "Italian Style" Green Beans Green Beans Nicoise Egg Plant Parmesan or Zucchini Parmesan Club Spinach Dinner: Cheese Ravioli (frozen) or...Beef Ravioli (frozen) Cheese Manicotti (frozen) or Beef Manicotti (frozen) Meat Sauce (for ravioli and manicotti) Spaghetti w/ Meatballs ...or reconstituted, and seasons food as required. Includes preparing eggs , hot cakes, french toast, meats, and other items on the serving line grill

Personality in frozen shoulder

PubMed Central

Fleming, A.; Dodman, Sally; Beer, T. C.; Crown, S.

1976-01-01

Fleming, A., Dodman, S., Beer, T. C., and Crown, S. (1976).Annals of the Rheumatic Diseases, 35, 456-457. Personality in frozen shoulder. Fifty-six patients with frozen shoulder have had their personality profiles investigated by means of the Middlesex Hospital Questionnaire. Females showed significantly increased somatic anxiety compared with controls. It is suggested that this may be important both to aetiology and treatment. Males and females should be assessed separately in future studies of frozen shoulder. PMID:1234412

Muscle Fiber Orientation Angle Dependence of the Tensile Fracture Behavior of Frozen Fish Muscle

NASA Astrophysics Data System (ADS)

Hagura, Yoshio; Okamoto, Kiyoshi; Suzuki, Kanichi; Kubota, Kiyoshi

We have proposed a new cutting method for frozen fish named "cryo-cutting". This method applied tensile fracture force or bending fracture force to the frozen fish at appropriate low temperatures. In this paper, to clarify cryo-cutting mechanism, we analyzed tensile fracture behavior of the frozen fish muscle. In the analysis, the frozen fish muscle was considered unidirectionally fiber-reinforced composite material which consisted of fiber (muscle fiber) and matrix (connective tissue). Fracture criteria (maximum stress criterion, Tsai-Hill criterion) for the unidirectionally fiber-reinforced composite material were used. The following results were obtained: (1) By using Tsai-Hill criterion, muscle fiber orientation angle dependence of the tensile fracture stress could be calculated. (2) By using the maximum stress theory jointly with Tsai-Hill criterion, muscle fiber orientation angle dependence of the fracture mode of the frozen fish muscle could be estimated.

Effects of Storage Temperatures on the Quality of Frozen Sardine, Mackerel, and Saury

NASA Astrophysics Data System (ADS)

Kozima, Tsuneo; Ohataka, Tateo

The three Japanese coastal fish species, sardine (Sardinops melanosticta), mackerel (Scomber japonicus) and saury (Cololabis saira) was frozen under commercial condition and stored at -18, -23, -30 and -40°C for 12 months. During frozen storage the quality was measured by determining the K value (freshness index), peroxide value (POV) of fat, a mount of free drip, water-holding capacity of muscle, weight ratio of cooking loss, histological feature of frozen and thawed muscle, and organoleptic test at regular intervals (each 2 months). Storage life of frozen sardine was 6 months at -18°C and 12 months at below -23°C. On frozen mackerel it was 6 months at -18°C. 8 months at -23°C and 12 months at below -30°C. On saury it was 6 months at 18°C and 12 months at below -23°C.

The value of frozen cartilage tissues without cryoprotection for genetic conservation.

PubMed

Cetinkaya, Gaye; Hatipoglu, Ibrahim; Arat, Sezen

2014-02-01

Animal tissues frozen without cryoprotection are thought to be inappropriate for use as a donor for somatic cell nuclear transfer (SCNT) studies. Cells in tissues that have been frozen without a cryoprotectant are commonly thought to be dead or to have lost genomic integrity. However, in this study we show that the frozen auricular cartilage tissues of anatolian buffalo contain a considerable number of viable healthy cells. The cells in auricular cartilage tissues are resistant to cryo-injury at -80°C. Primary cell cultures were established from defrosted ear tissues which were frozen without cryoprotectant. The growth and functional characteristics of primary cell cultures are characterized according to cell growth curve, cell cycle analysis, karyotype and GAG synthesis. The results indicate that frozen cartilage tissues could be valuable materials for the conservation of species and SCNT technology. Copyright © 2013 Elsevier Inc. All rights reserved.

Parallel Implementation of a Frozen Flow Based Wavefront Reconstructor

NASA Astrophysics Data System (ADS)

Nagy, J.; Kelly, K.

2013-09-01

Obtaining high resolution images of space objects from ground based telescopes is challenging, often requiring the use of a multi-frame blind deconvolution (MFBD) algorithm to remove blur caused by atmospheric turbulence. In order for an MFBD algorithm to be effective, it is necessary to obtain a good initial estimate of the wavefront phase. Although wavefront sensors work well in low turbulence situations, they are less effective in high turbulence, such as when imaging in daylight, or when imaging objects that are close to the Earth's horizon. One promising approach, which has been shown to work very well in high turbulence settings, uses a frozen flow assumption on the atmosphere to capture the inherent temporal correlations present in consecutive frames of wavefront data. Exploiting these correlations can lead to more accurate estimation of the wavefront phase, and the associated PSF, which leads to more effective MFBD algorithms. However, with the current serial implementation, the approach can be prohibitively expensive in situations when it is necessary to use a large number of frames. In this poster we describe a parallel implementation that overcomes this constraint. The parallel implementation exploits sparse matrix computations, and uses the Trilinos package developed at Sandia National Laboratories. Trilinos provides a variety of core mathematical software for parallel architectures that have been designed using high quality software engineering practices, The package is open source, and portable to a variety of high-performance computing architectures.

Life in ice: implications to astrobiology

NASA Astrophysics Data System (ADS)

Hoover, Richard B.; Pikuta, Elena V.

2009-08-01

During previous research expeditions to Siberia, Alaska and Antarctica, it was observed that glaciers and ice wedges contained bacterial cells that became motile as soon as the ice melted. This phenomenon of live bacteria in ice was first documented for microbes in ancient ice cores from Vostok, Antarctica. The first validly published species of Pleistocene bacteria alive on Earth today was Carnobacterium pleistocenium. This extremophile had remained for 32,000 years, encased in ice recently exposed in the Fox Tunnel of Alaska. These frozen bacteria began to swim as soon as the ice was thawed. Dark field microscopy studies revealed that large numbers of bacteria exhibited motility as soon as glacial ice was melted during our recent Expeditions to Alaska and Antarctica led to the conclusion that microbial life in ice was not a rare phenomenon. The ability of bacteria to remain alive while frozen in ice for long periods of time is of great significance to Astrobiology. In this paper, we describe the recent observations and advance the hypothesis that life in ice provides valuable clues to how we can more easily search for evidence of life on the Polar Caps of Mars, comets and other icy bodies of our Solar System. It is suggested that cryopanspermia may have played a far more important role in Origin of Life on Earth and the distribution of Life throughout the Cosmos and than previously thought possible.

Perturbation propagation in random and evolved Boolean networks

NASA Astrophysics Data System (ADS)

Fretter, Christoph; Szejka, Agnes; Drossel, Barbara

2009-03-01

In this paper, we investigate the propagation of perturbations in Boolean networks by evaluating the Derrida plot and its modifications. We show that even small random Boolean networks agree well with the predictions of the annealed approximation, but nonrandom networks show a very different behaviour. We focus on networks that were evolved for high dynamical robustness. The most important conclusion is that the simple distinction between frozen, critical and chaotic networks is no longer useful, since such evolved networks can display the properties of all three types of networks. Furthermore, we evaluate a simplified empirical network and show how its specific state space properties are reflected in the modified Derrida plots.

Spectral Density of Laser Beam Scintillation in Wind Turbulence. Part 1; Theory

NASA Technical Reports Server (NTRS)

Balakrishnan, A. V.

1997-01-01

The temporal spectral density of the log-amplitude scintillation of a laser beam wave due to a spatially dependent vector-valued crosswind (deterministic as well as random) is evaluated. The path weighting functions for normalized spectral moments are derived, and offer a potential new technique for estimating the wind velocity profile. The Tatarskii-Klyatskin stochastic propagation equation for the Markov turbulence model is used with the solution approximated by the Rytov method. The Taylor 'frozen-in' hypothesis is assumed for the dependence of the refractive index on the wind velocity, and the Kolmogorov spectral density is used for the refractive index field.

Production Guides for Meat and Vegetable Entrees and Desserts Developed for Use in the Frozen Foil Pack Feeding System, F.E. Warren Air Force Base

DTIC Science & Technology

1976-02-01

8217-i~ ", i " ’ im i-II U BAKED BEEF WITH NOODLS 1i1 Yield: 100 Portions Each Portion: 8 ounces Ingredients Pounds Grams Procedure Beef, boneless, diced...simmer for approxi- mately 1 hour. Noodles , dry 4.00 1,816 2. Approximately * hour prior to completion of beef cook, start cooking noodles in a separate...tap 27.00 12,260 ture thickens (cook 15 minutes). 4. Add noodles from step 2; mix well and adjust volume to 7.8 gallons. Heat to 180 F.*2 5. Weigh 8

Instabilities in rapid solidification of multi-component alloys

NASA Astrophysics Data System (ADS)

Altieri, Anthony L.; Davis, Stephen H.

2017-10-01

Rapid solidification of multi-component liquids occurs in many modern applications such as additive manufacturing. In the present work the interface departures from equilibrium consist of the segregation coefficient and liquidus slope depending on front speed, the one-sided, frozen-temperature approximation, and the alloy behaving as the superposition of individual components. Linear-stability theory is applied, showing that the cellular and oscillatory instabilities of the binary case are modified. The addition of components tends to destabilize the interface while the addition of a single large-diffusivity material can entirely suppress the oscillatory mode. Multiple minima in the neutral curve for the cellular mode occur.

Theoretical Performance of Hydrogen-Oxygen Rocket Thrust Chambers

NASA Technical Reports Server (NTRS)

Sievers, Gilbert K.; Tomazic, William A.; Kinney, George R.

1961-01-01

Data are presented for liquid-hydrogen-liquid-oxygen thrust chambers at chamber pressures from 15 to 1200 pounds per square inch absolute, area ratios to approximately 300, and percent fuel from about 8 to 34 for both equilibrium and frozen composition during expansion. Specific impulse in vacuum, specific impulse, combustion-chamber temperature, nozzle-exit temperature, characteristic velocity, and the ratio of chamber-to-nozzle-exit pressure are included. The data are presented in convenient graphical forms to allow quick calculation of theoretical nozzle performance with over- or underexpansion, flow separation, and introduction of the propellants at various initial conditions or heat loss from the combustion chamber.

Physics behind the mechanical nucleosome positioning code

NASA Astrophysics Data System (ADS)

Zuiddam, Martijn; Everaers, Ralf; Schiessel, Helmut

2017-11-01

The positions along DNA molecules of nucleosomes, the most abundant DNA-protein complexes in cells, are influenced by the sequence-dependent DNA mechanics and geometry. This leads to the "nucleosome positioning code", a preference of nucleosomes for certain sequence motives. Here we introduce a simplified model of the nucleosome where a coarse-grained DNA molecule is frozen into an idealized superhelical shape. We calculate the exact sequence preferences of our nucleosome model and find it to reproduce qualitatively all the main features known to influence nucleosome positions. Moreover, using well-controlled approximations to this model allows us to come to a detailed understanding of the physics behind the sequence preferences of nucleosomes.

A preliminary assessment of the impact of 2-D exhaust-nozzle geometry on the cruise range of a hypersonic aircraft with top-mounted ramjet propulsion

NASA Technical Reports Server (NTRS)

Vahl, W. A.; Weidner, J. P.

1980-01-01

A theoretical study of full length and shortened, two dimensional, isentropic, exhaust nozzles integrated with top mounted ramjet propulsion nacelles were conducted. Both symmetric and asymmetric contoured nozzles with a range of angular orientations were considered. Performance comparisons to determine optimum installations for a representative hypersonic vehicle at Mach 5 cruise conditions are presented on the basis of cruise range, propulsive specific impulse, inlet area requirements, and overall lift drag ratio. The effect of approximating the nozzle internal contours with planar surfaces and the determination of viscous and frozen flow effects are also presented.

Indication and method of frozen section in vaginal radical trachelectomy.

PubMed

Chênevert, Jacinthe; Têtu, Bernard; Plante, Marie; Roy, Michel; Renaud, Marie-Claude; Grégoire, Jean; Grondin, Katherine; Dubé, Valérie

2009-09-01

Vaginal radical trachelectomy (VRT) is a fertility-sparing surgical technique used as an alternative to radical hysterectomy in early stage cervical carcinoma. With the advent of VRT, preoperative evaluation of the surgical margin has become imperative, because if the tumor is found within 5 mm of the endocervical margin, additional surgical resection is required. In a study published earlier from our center, we came to the conclusion that a frozen section should be conducted only when a cancerous lesion is grossly visible, and that it could be omitted in normal-looking specimens or VRT with nonspecific lesions. Since then, 53 VRT have been performed in our center, and frozen sections were conducted according to these recommendations. Fifteen VRT were grossly normal, 24 had a nonspecific lesion and 14 showed a grossly visible lesion. Final margins were satisfactory on all 15 grossly normal specimens. Of the 24 VRT with nonspecific lesions, 2 cases for which no frozen section was performed had unsatisfactory final margins (<5 mm). Of the 14 VRT with grossly visible lesions, 3 cases were inadequately evaluated by frozen section due to sampling errors, which led to unsatisfactory final margin assessment. These results confirm that a frozen section can be omitted on normal looking VRT specimens, but contrary to results published earlier, we recommend that a frozen section be performed on all VRT with nonspecific lesions. As for VRT with a grossly visible lesion, frozen section evaluation is still warranted, and we recommend increasing the sampling to improve the adequacy of frozen sections.

Detection of Ketenimine (CH2CNH) in SGRB2(N) Hot Cores

NASA Technical Reports Server (NTRS)

Lovas, F. J.; Hollis, J. M.; Remijan, Anthony J.; Jewell, P. R.

2006-01-01

Ketenimine (CH2CNH) has been detected in absorption toward the starforming region Sagittarius B2(N) with the 100-m Green Bank Telescope (GBT) by means of three rotational transitions: 7(sub 16)-8(sub 08) at 41.5 GHz, 8(sub 19)-9(sub 09), at 23.2 GHz, and 9(sub 18)-10(sub 0,10) at 4.9 GHz. Ketenimine has a sparse rotational spectrum below 50 GHz. From transition line strength arguments, the spectral lines found are the ones most likely to be detected and occur in spectral regions that have little possibility of confusion with other molecular species. Partially resolved hyperfine structure is apparent in the 4.9 GHz transition which has energy levels approximately 50 K above ground state level; the absorption seen in this transition appears to be emanating from gas in close proximity to the LMH hot core that has a systemic LSR velocity of +64 kilometers per second. By comparison, the 41.5 GHz and 23.2 GHz transitions have lower energy levels of approximately 33 K and approximately 41 K, respectively; and show absorption against the two star-forming SgrB2(N) hot cores with systematic LSR velocities of +64 (the LMH) and +82 kilometers per second. These ketenimine data show that the hot core at +82 kilometers per second is cooler than the hot core at +64 kilometers per second. Ketenimine is likely formed directly from its isomer methyl cyanide (CH3CN) by tautomerization driven by shocks that pervade the star-forming region.

Numerical studies of the margin of vortices with decaying cores

NASA Technical Reports Server (NTRS)

Liu, G. C.; Ting, L.

1986-01-01

The merging of vortices to a single one is a canonical incompressible viscous flow problem. The merging process begins when the core sizes or the vortices are comparable to their distances and ends when the contour lines of constant vorticity lines are circularized around one center. Approximate solutions to this problem are constructed by adapting the asymptotic solutions for distinct vortices. For the early stage of merging, the next-order terms in the asymptotic solutions are added to the leading term. For the later stage of merging, the vorticity distribution is reinitialized by vortices with overlapping core structures guided by the 'rule of merging' and the velocity of the 'vortex centers' are then defined by a minimum principle. To show the accuracy of the approximate solution, it is compared with the finite-difference solution.

A new equation of state Based on Nuclear Statistical Equilibrium for Core-Collapse Simulations

NASA Astrophysics Data System (ADS)

Furusawa, Shun; Yamada, Shoichi; Sumiyoshi, Kohsuke; Suzuki, Hideyuki

2012-09-01

We calculate a new equation of state for baryons at sub-nuclear densities for the use in core-collapse simulations of massive stars. The formulation is the nuclear statistical equilibrium description and the liquid drop approximation of nuclei. The model free energy to minimize is calculated by relativistic mean field theory for nucleons and the mass formula for nuclei with atomic number up to ~ 1000. We have also taken into account the pasta phase. We find that the free energy and other thermodynamical quantities are not very different from those given in the standard EOSs that adopt the single nucleus approximation. On the other hand, the average mass is systematically different, which may have an important effect on the rates of electron captures and coherent neutrino scatterings on nuclei in supernova cores.

Few-body modes of binary formation in core collapse

NASA Astrophysics Data System (ADS)

Tanikawa, Ataru; Heggie, Douglas C.; Hut, Piet; Makino, Junichiro

2013-11-01

At the moment of deepest core collapse, a star cluster core contains less than ten stars. This small number makes the traditional treatment of hard binary formation, assuming a homogeneous background density, suspect. In a previous paper, we have found that indeed the conventional wisdom of binary formation, based on three-body encounters, is incorrect. Here we refine that insight, by further dissecting the subsequent steps leading to hard binary formation. For this purpose, we add some analysis tools in order to make the study less subjective. We find that the conventional treatment does remain valid for direct three-body scattering, but fails for resonant three-body scattering. Especially democratic resonance scattering, which forms an important part of the analytical theory of three-body binary formation, takes too much space and time to be approximated as being isolated, in the context of a cluster core around core collapse. We conclude that, while three-body encounters can be analytically approximated as isolated, subsequent strong perturbations typically occur whenever those encounters give rise to democratic resonances. We present analytical estimates postdicting our numerical results. If we only had been a bit more clever, we could have predicted this qualitative behaviour.

FROZEN RAW FOODS AS SKIN-TESTING MATERIALS—Further Studies of Use in Cases of Allergic Disorders

PubMed Central

Ancona, Giacomo R.; Schumacher, Irwin C.

1954-01-01

In further studies on the use of frozen raw food as skin-testing material in patients with allergic disorders, the results of previous work were confirmed in a greater number of subjects using a larger number of foods: Tests with frozen raw foods by the scratch method induce true positive reactions of a larger size and in greater frequency than the corresponding commercial extracts by either the scratch or the intracutaneous method. Storage in the frozen state for several years does not affect the antigenic potency of the materials. The frozen preparations have caused no harmful effects in the subjects, are free from irritant properties, and are not urticariogenic. PMID:13126823

A 300 year history of lead contamination in northern French Alps reconstructed from distant lake sediment records.

PubMed

Arnaud, F; Revel-Rolland, M; Bosch, D; Winiarski, T; Desmet, M; Tribovillard, N; Givelet, N

2004-05-01

Lead concentrations and isotopic ratios were measured along two well-dated sediment cores from two distant lakes: Anterne (2100 m a.s.l.) and Le Bourget (270 m a.s.l.), submitted to low and high direct human impact and covering the last 250 and 600 years, respectively. The measurement of lead in old sediment samples (>3000 BP) permits, in using mixing-models, the determination of lead concentration, flux and isotopic composition of purely anthropogenic origin. We thus show that since ca. 1800 AD the regional increase in lead contamination was mostly driven by coal consumption ((206)Pb/(207)Pb approximately 1.17-1.19; (206)Pb/(204)Pb approximately 18.3-18.6), which peaks around 1915 AD. The increasing usage of leaded gasoline, introduced in the 1920s, was recorded in both lakes by increasing Pb concentrations and decreasing Pb isotope ratios. A peak around 1970 ((206)Pb/(207)Pb approximately 1.13-1.16; (206)Pb/(204)Pb approximately 17.6-18.0) corresponds to the worldwide recorded leaded gasoline maximum of consumption. The 1973 oil crisis is characterised by a drastic drop of lead fluxes in both lakes (from approximately 35 to <20 mg cm(-2) yr(-1)). In the late 1980s, environmental policies made the Lake Anterne flux drop to pre-1900 values (<10 mg cm(-2) yr(-1)) while Lake Le Bourget is always submitted to an important flux (approximately 25 mg cm(-2) yr(-1)). The good match of our distant records, together and with a previously established series in an ice core from Mont Blanc, provides confidence in the use of sediments as archives of lead contamination. The integration of the Mont Blanc ice core results from Rosman et al. with our data highlights, from 1990 onward, a decoupling in lead sources between the high elevation sites (Lake Anterne and Mont Blanc ice core), submitted to a mixture of long-distance and regional contamination and the low elevation site (Lake Le Bourget), where regional contamination is predominant.

Geochemical Comparison of Four Cores from the Manson Impact Structure

NASA Technical Reports Server (NTRS)

Korotev, Randy L.; Rockow, Kaylynn M.; Jolliff, Bradley L.; Haskin, Larry A.; McCarville, Peter; Crossey, Laura J.

1996-01-01

Concentrations of 33 elements were determined in relatively unaltered, matrix-rich samples of impact breccia at approximately 3-m-depth intervals in the M-1 core from the Manson impact structure, Iowa. In addition, 46 matrix-rich samples from visibly altered regions of the M-7, M-8, and M-10 cores were studied, along with 42 small clasts from all four cores. Major element compositions were determined for a subset of impact breccias from the M-1 core, including matrix-rich impact-melt breccia. Major- and trace-element compositions were also determined for a suite of likely target rocks. In the M-1 core, different breccia units identified from lithologic examination of cores are compositionally distinct. There is a sharp compositional discontinuity at the boundary between the Keweenawan-shale-clast breccia and the underlying unit of impact-melt breccia (IMB) for most elements, suggesting minimal physical mixing between the two units during emplacement. Samples from the 40-m-thick IMB (M-1) are all similar to each other in composition, although there are slight increases in concentration with depth for those elements that have high concentrations in the underlying fragmental-matrix suevite breccia (SB) (e.g., Na, Ca, Fe, Sc), presumably as a result of greater clast proportions at the bottom margin of the unit of impact-melt breccia. The high degree of compositional similarity we observe in the impact-melt breccias supports the interpretation that the matrix of this unit represents impact melt. That our analyses show such compositional similarity results in part from our technique for sampling these breccias: for each sample we analyzed a few small fragments (total mass: approximately 200 mg) selected to be relatively free of large clasts and visible signs of alteration instead of subsamples of powders prepared from a large mass of breccia. The mean composition of the matrix-rich part of impact-melt breccia from the M-1 core can be modeled as a mixture of approximately 35% shale and siltstone (Proterozoic "Red Clastics"), 23% granite, 40% hornblende-biotite gneiss, and a small component (less than 2%) of mafic-dike rocks.

Hazardous Waste Cleanup: Chevron Phillips Chemical Puerto Rico Core in Guayama, Puerto Rico

EPA Pesticide Factsheets

Chevron Phillips Chemical Puerto Rico Core (CPCPRC) plant is located on Road 710, km 1.3, approximately 2.5 miles southwest of Guayama, Puerto Rico, and northeast of Las Mareas Harbor. CPCPRC has operated at the Guayama site since 1966. The Guamani River

Presentation of frozen shoulder among diabetic and non-diabetic patients☆

PubMed Central

Uddin, Mohammad Moin; Khan, Aminuddin A.; Haig, Andrew J.; Uddin, Mohammad Kafil

2014-01-01

Objective The literature is inconsistent regarding the level of pain and disability in frozen shoulder patients with or without diabetes mellitus. The aim of this study is to evaluate some demographic features of frozen shoulder patients and to look into the disparity of information by comparing the level of pain and disability due to frozen shoulder between diabetic and non-diabetic people. Design This is a prospective comparative study. People with frozen shoulder attending an outpatient department were selected by consecutive sampling. Disability levels were assessed by the Shoulder Pain & Disability Index (SPADI). Means of pain and disability scores were compared using unpaired t-test. Results Among 140 persons with shoulder pain 99 (71.4%) had frozen shoulder. From the participating 40 frozen shoulder patients, 26 (65%) were males and 14 (35%) were females. Seventeen participants (42.5%) were diabetic, two (5%) had impaired glucose tolerance and 21 (52.5%) patients were non-diabetic. Mean disability scores (SPADI) were 51 ± 15.5 in diabetic and 57 ± 16 in non-diabetic persons. The differences in pain and disability level were not statistically significance (respectively, p = 0.24 and p = 0.13 at 95% confidence interval). Conclusions No difference was found in level of pain and disability level between frozen shoulder patients with and without diabetes. PMID:25983497

78 FR 19639 - Certain Frozen Warmwater Shrimp From India and Thailand: Notice of Initiation of Antidumping Duty...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-02

... Gallant Seafoods Corporation Global Frozen Food (Thailand) Co Global Maharaja Co., Ltd Golden Seafood... Frozen Foods Co., Ltd Lee Heng Seafood Co., Ltd Leo Global Logistics Co., Ltd Leo Transports Li-Thai...

Frozen section analysis of margins for head and neck tumor resections: reduction of sampling errors with a third histologic level.

PubMed

Olson, Stephen M; Hussaini, Mohammad; Lewis, James S

2011-05-01

Frozen section analysis is an essential tool for assessing margins intra-operatively to assure complete resection. Many institutions evaluate surgical defect edge tissue provided by the surgeon after the main lesion has been removed. With the increasing use of transoral laser microsurgery, this method is becoming even more prevalent. We sought to evaluate error rates at our large academic institution and to see if sampling errors could be reduced by the simple method change of taking an additional third section on these specimens. All head and neck tumor resection cases from January 2005 through August 2008 with margins evaluated by frozen section were identified by database search. These cases were analyzed by cutting two levels during frozen section and a third permanent section later. All resection cases from August 2008 through July 2009 were identified as well. These were analyzed by cutting three levels during frozen section (the third a 'much deeper' level) and a fourth permanent section later. Error rates for both of these periods were determined. Errors were separated into sampling and interpretation types. There were 4976 total frozen section specimens from 848 patients. The overall error rate was 2.4% for all frozen sections where just two levels were evaluated and was 2.5% when three levels were evaluated (P=0.67). The sampling error rate was 1.6% for two-level sectioning and 1.2% for three-level sectioning (P=0.42). However, when considering only the frozen section cases where tumor was ultimately identified (either at the time of frozen section or on permanent sections) the sampling error rate for two-level sectioning was 15.3 versus 7.4% for three-level sectioning. This difference was statistically significant (P=0.006). Cutting a single additional 'deeper' level at the time of frozen section identifies more tumor-bearing specimens and may reduce the number of sampling errors.

Increased risk of large-for-gestational age birthweight in singleton siblings conceived with in vitro fertilization in frozen versus fresh cycles.

PubMed

Luke, Barbara; Brown, Morton B; Wantman, Ethan; Stern, Judy E; Toner, James P; Coddington, Charles C

2017-02-01

Children born from fresh in vitro fertilization (IVF) cycles are at greater risk of being born smaller and earlier, even when limited to singletons; those born from frozen cycles have an increased risk of large-for-gestational age (LGA) birthweight (z-score ≥1.28). This analysis sought to overcome limitations in other studies by using pairs of siblings, and accounting for prior cycle outcomes, maternal characteristics, and embryo state and stage. Pairs of singleton births conceived with IVF and born between 2004 and 2013 were identified from the Society for Assisted Reproductive Technology Clinic Outcome Reporting System database, matched for embryo stage (blastocyst versus non-blastocyst) and infant gender, categorized by embryo state (fresh versus frozen) in 1st and 2nd births (four groups). The data included 7795 singleton pairs. Birthweight z-scores were 0.00-0.04 and 0.24-0.26 in 1st and 2nd births in fresh cycles, and 0.25-0.34 and 0.50-0.55 in frozen cycles, respectively. LGA was 9.2-9.8 and 14.2-15.4% in 1st and 2nd births in fresh cycles, and 13.1-15.8 and 20.8-21.0% in 1st and 2nd births in frozen cycles. The risk of LGA was increased in frozen cycles (1st births, adjusted odds ratios (AOR) 1.74, 95% CI 1.45, 2.08; and in 2nd births when the 1st birth was not LGA, AOR 1.70, 95% CI 1.46, 1.98 for fresh/frozen and 1.40, 1.11, 1.78 for frozen/frozen). Our results with siblings indicate that frozen embryo state is associated with an increased risk for LGA. The implications of these findings for childhood health and risk of obesity are unclear, and warrant further investigation.

Effects of type of freeze straw and thaw temperature on the fertilizing capacity of frozen chicken semen.

PubMed

Duplaix, M; Sexton, T J

1984-04-01

Two experiments were conducted to determine the relationship between thawing temperature and the type of straw in which chicken semen was frozen. In Experiment 1, semen was frozen in three different types of plastic straws: US (.5-ml capacity), French (.5-ml capacity), and French mini (.25-ml capacity). Experiment 1 was divided into two trials to compare semen packaged in the different straws and thawed at 15 (Trial 1) or .5 C (Trial 2). Although there were distinguishable features of the freeze and thaw curves between samples frozen in the different straws, the type of freeze straw had no effect on the fertilizing capacity of frozen semen when thaw temperature was held constant: fertility, Days 2 to 4 after artificial insemination, ranged from 16 to 27% for Trial 1 and 45 to 47% for Trial 2. In Experiment 2, semen was frozen in US straws and thawed at either .5 or 15 C to assess the effect of the thaw temperature. Fertility of frozen semen, Days 2 to 4 after artificial insemination, was significantly higher when semen was thawed at .5 than at 15 C (62 vs. 20%).

Rates of surgery for frozen shoulder: an experience in England.

PubMed

Kwaees, Tariq A; Charalambous, Charalambos P

2015-01-01

the aim of this study was to identify the incidence of surgical treatment for frozen shoulder in a western population. patients included in this study all resided within a well-defined area in the North West of England, all had surgery for frozen shoulder over a 3-year period and were identified from theatre logbooks of two local hospitals. Cases having surgery for shoulder stiffness other than frozen shoulder were excluded. Local and national population size estimates were based on data obtained from the UK Office for National Statistics. 117 patients underwent surgery for frozen shoulder during the period examined; of these 101 had arthroscopic arthrolysis and 16 had manipulation under anaesthesia. The overall incidence of frozen shoulder surgery was calculated at 2.67 procedures per 10,000 general population per year, and at 7.55 for those aged 40-60. surgical intervention for frozen shoulder is common, estimated at over 14,180 cases per year in England. Given the variation in costs associated with arthroscopic arthrolysis and manipulation under anaesthesia, comparative studies of the cost effectiveness of the two procedures would be of great value. 2C (outcome research).

Effect of long term chilled (up to 5weeks) then frozen (up to 12months) storage at two different sub-zero holding temperatures on beef: 2. Lipid oxidation and fatty acid profiles.

PubMed

Holman, Benjamin W B; Coombs, Cassius E O; Morris, Stephen; Bailes, Kristy; Hopkins, David L

2018-02-01

Lipid oxidation and fatty acid (FA) profiles were evaluated for beef M. longissimus lumborum (LL) stored under different chilled-then-frozen storage combinations (up to 5 and 52weeks, respectively) and two frozen holding temperatures (-12°C and -18°C). FA profile variation was observed, with increasing frozen storage periods resulting in unsaturated FA levels declining as saturated FA levels increased. Polyunsaturated and health claimable FA levels also tended to decline with increasing chilled storage period, albeit insignificant within the constraints of the experimental design. Peroxidase activity, TBARS and oxidation-reduction potential analyses reflected these FA changes. These, when compared against existing consumer thresholds, suggest a perceptible detraction from LL held under long-term frozen storage durations that are less evident earlier as dependent on the preceding chilled storage period. Negligible impact of frozen storage holding temperatures was observed on measured traits. These results suggest long-term chilled-then-frozen storage can influence beef lipid stability, healthy FA profile and therefore the healthiness of beef. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

Fertility test of frozen boar semen.

PubMed

Osinowa, O; Salamon, S

1976-10-01

The fertility results of two experiments are presented. In experiment 1, the semen was frozen in tris-fructose-EDTA or BF3 diluents at 0-25 X 10(9)/ml sperm concentration and extended after thawing with either seminal plasma (SP) or the freezing medium (FM) containing no cryoprotective agent. In the second experiment the semen was glycerolated by two methods, frozen at 1-0 X 10(9)/ml sperm concentration, and extended wtih FM before insemination. Fertility after double insemination within one oestrus with semen frozen in tris-fructose-EDTA or BF3 diluents varied depending on the medium used for extension of thawed semen. The farrowing rates for semen frozen in the former diluent with FM and SP post-thawing media were 4/8 and 1/8 respectively, and for semen frozen BF3 diluent with FM and SP post-thawing extenders 1/8 and 5/8. The mean farrowing for the 32 animals inseminasted was 34-4%. Pregnancies for semen frozen in tris-fructose-EDTA and glycerolated at 30 or 5 degrees C were 5/12 and 4/12 respectively, and for single and double inseminations 6/12 and 3/12 respectively. Of 24 animals inseminated 37-5% farrowed.

Effect of Chitin Isolated from Crustaceans and Cephalopods on Denaturation of Fish Myofibrillar Protein and the State of Water during Frozen Storage

NASA Astrophysics Data System (ADS)

Yamashita, Yasumitsu; Zhang, Nong; Nozaki, Yukinori

The effect of chitin hydrolysate made from shell of crustaceans and cartilage of cephalopods on the denaturation and the state of water of lizard fish myofibrillar protein (Mf) during frozen storage at -250°C for 120 days were investigated. 5.0% chitin hydrolysate (dried matter) added Mf samples were frozen at -25°C and storage for 120 days, and the change of inactivation of Mf Ca-ATPase and the change of unfrozen water in Mf were examined during the frozen storage. The decrease of Mf Ca-ATPase during frozen storage were slow by addition of chitin hydrolysate. The amount of unfrozen water in Mf were increased by addition of chitin hydrolysate, and decreaced gradually during frozen storage. On the other hand, the amount of unfrozen water of the control were decreaced suddenly during frozen storage. Above results suggest that chitin hydrolysate has cryoprotective effect on Mf. It is guessed that the mechanism of cryoprotective effect of chitin hydrolysate may be caused by an interaction between hydration sphere of Mf and equatorial OH groups in the molecular structure of chitin hydrolysate.

75 FR 76754 - Non-Frozen Apple Juice Concentrate From China

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-09

... INTERNATIONAL TRADE COMMISSION [Investigation No. 731-TA-841 (Second Review)] Non-Frozen Apple Juice Concentrate From China AGENCY: United States International Trade Commission. ACTION: Termination... whether revocation of the antidumping duty order on non- frozen apple juice concentrate from China would...

Indications for hydrodilatation for frozen shoulder

PubMed Central

Rymaruk, S.; Peach, C.

2017-01-01

Frozen shoulder causes significant functional disability and pain in a population group constituted by patients who are often middle-aged and working. Frozen shoulder remains poorly understood. The available literature is limited and often prone to bias. A rapid, non-surgical and cost-effective treatment that reduces pain and restores function is an attractive option. Hydrodilatation is a potential first-line treatment of frozen shoulder in secondary care. Cite this article: EFORT Open Rev 2017;2:462–468. DOI: 10.1302/2058-5241.2.160061 PMID:29218231

The Mechanical Properties of Wood of Different Moisture Content Within -200 Degrees to +200 Degrees C Temperature Range

NASA Technical Reports Server (NTRS)

Kollmann, Franz

1941-01-01

Systematic experiments were undertaken with special reference to the effect of gross specific weight (specific weight inclusive of pores) and the moisture content of wood. It was found that the modules of elasticity of wood at room temperature and frozen at -8 degrees is practically the same. The effect of moisture on the compression strength of frozen wood was explored as well as the flexural and impact strength of frozen wood and frozen laminated wood.

Frozen and fresh ovarian tissue require different culture media to promote in vitro development of bovine preantral follicles.

PubMed

Castro, Simone Vieira; Carvalho, Adeline Andrade; Silva, Cleidson Manoel Gomes; Santos, Francielli Weber; Campello, Cláudio Cabral; de Figueiredo, José Ricardo; Rodrigues, Ana Paula Ribeiro

2014-10-01

The aim of this study was to evaluate the efficiency of different media in the in vitro culture of bovine preantral follicles that were used either fresh or following slow freezing treatment. Frozen and fresh noncultured or cultured ovarian fragments were processed for histological, viability, and cell proliferation analyses. For cryopreservation, a solution containing 1.5 M ethylene glycol was frozen in a programmable biological freezer. After thawing, a portion of the samples was destined for frozen controls. The remainder were cultured in vitro for 5 days in three media: α-MEM, McCoy, or M199. Samples from these culture media were collected on days 1 and 5 for quantification of reactive oxygen species (ROS) and for hormonal assays. In fresh-cultured tissues, the percentage of morphologically normal follicles was significantly higher when cultured in M199 compared to that in the other media. In frozen-cultured tissues, McCoy medium was significantly superior to the other media, and was the only treatment that helped in maintaining the viability similar to fresh and frozen controls. Upon quantification of the nucleolus organizer region, we observed greater proliferation of granulosa cells in the frozen-cultured tissues with McCoy medium, and lesser proliferation in fresh-cultured tissues only with α-MEM. In frozen-cultured tissues, ROS levels were highest at day 1 and progressively reduced during culture, independent of the media used. In conclusion, under the conditions used in this study, the M199 and McCoy media are recommended for the culture of follicles derived from fresh and frozen ovarian tissues, respectively.

Effect of blanching treatments on antioxidant activity of frozen green capsicum (Capsicum annuum L. var bell pepper) using radical scavenging activity (DPPH) assay

NASA Astrophysics Data System (ADS)

Azizzuddin, Norafida; Abdullah, Aminah

2016-11-01

Blanching treatments are needed to deactivate enzymes in frozen vegetables. Antioxidant activity using DPPH radical scavenging activity assay were evaluated in steaming, boiling water, and microwave blanching at different temperature, time and microwave power level on frozen green capsicum. Green capsicum was chosen for frozen treatment compared to other capsicum with different maturity index because of the firm texture. The objective of this study was to compare the antioxidant activity of frozen green capsicum between conventional and Oxi Count Kit® assay for DPPH radical scavenging activity. Results showed frozen green capsicum blanched using microwave at high level/90 seconds (sample J) contained higher level of DPPH in both conventional method and Oxi Count Kit® compared to other treatments. However, there were no significant differences between sample J and fresh sample (sample A). Overall, the sequences from highest to lowest in blanching treatments for both DPPH conventional method, and DPPH Oxi Count Kit® were J (microwave high level/90 seconds) > A (Fresh) > H (Microwave Medium Level/120 seconds) > D (Boiling Water 80°C/150 seconds) > K (Microwave High Level/120 seconds) > I (Microwave Medium Level/150 seconds) > F (Microwave Low Level/150 seconds)> B (Steam 100°C/150 seconds) > E (Boiling Water 100°C /120 seconds) > G (Microwave Low Level /180 seconds)> C (Steam 100°C/180 seconds). Almost all frozen green capsicum samples showed no significant differences for comparison between test using DPPH conventional method and Oxi Count Kit®. Frozen storage for 0, and 3rd months showed no significant differences which indicate no changes on antioxidant activity during frozen storage at -18°C.

Quantifying the streamflow response to frozen ground degradation in the source region of the Yellow River within the Budyko framework

NASA Astrophysics Data System (ADS)

Wang, Taihua; Yang, Hanbo; Yang, Dawen; Qin, Yue; Wang, Yuhan

2018-03-01

The source region of the Yellow River (SRYR) is greatly important for water resources throughout the entire Yellow River Basin. Streamflow in the SRYR has experienced great changes over the past few decades, which is closely related to the frozen ground degradation; however, the extent of this influence is still unclear. In this study, the air freezing index (DDFa) is selected as an indicator for the degree of frozen ground degradation. A water-energy balance equation within the Budyko framework is employed to quantify the streamflow response to the direct impact of climate change, which manifests as changes in the precipitation and potential evapotranspiration, as well as the impact of frozen ground degradation, which can be regarded as part of the indirect impact of climate change. The results show that the direct impact of climate change and the impact of frozen ground degradation can explain 55% and 33%, respectively, of the streamflow decrease for the entire SRYR from Period 1 (1965-1989) to Period 2 (1990-2003). In the permafrost-dominated region upstream of the Jimai hydrological station, the impact of frozen ground degradation can explain 71% of the streamflow decrease. From Period 2 (1990-2003) to Period 3 (2004-2015), the observed streamflow did not increase as much as the precipitation; this could be attributed to the combined effects of increasing potential evapotranspiration and more importantly, frozen ground degradation. Frozen ground degradation could influence streamflow by increasing the groundwater storage when the active layer thickness increases in permafrost-dominated regions. These findings will help develop a better understanding of the impact of frozen ground degradation on water resources in the Tibetan Plateau.

Sensitivity of frozen section histology for identifying Propionibacterium acnes infections in revision shoulder arthroplasty.

PubMed

Grosso, Matthew J; Frangiamore, Salvatore J; Ricchetti, Eric T; Bauer, Thomas W; Iannotti, Joseph P

2014-03-19

Propionibacterium acnes is a clinically relevant pathogen with total shoulder arthroplasty. The purpose of this study was to determine the sensitivity of frozen section histology in identifying patients with Propionibacterium acnes infection during revision total shoulder arthroplasty and investigate various diagnostic thresholds of acute inflammation that may improve frozen section performance. We reviewed the results of forty-five patients who underwent revision total shoulder arthroplasty. Patients were divided into the non-infection group (n = 15), the Propionibacterium acnes infection group (n = 18), and the other infection group (n = 12). Routine preoperative testing was performed and intraoperative tissue culture and frozen section histology were collected for each patient. The histologic diagnosis was determined by one pathologist for each of the four different thresholds. The absolute maximum polymorphonuclear leukocyte concentration was used to construct a receiver operating characteristics curve to determine a new potential optimal threshold. Using the current thresholds for grading frozen section histology, the sensitivity was lower for the Propionibacterium acnes infection group (50%) compared with the other infection group (67%). The specificity of frozen section was 100%. Using a receiver operating characteristics curve, an optimized threshold was found at a total of ten polymorphonuclear leukocytes in five high-power fields (400×). Using this threshold, the sensitivity of frozen section for Propionibacterium acnes was increased to 72%, and the specificity remained at 100%. Using current histopathology grading systems, frozen sections were specific but showed low sensitivity with respect to the Propionibacterium acnes infection. A new threshold value of a total of ten or more polymorphonuclear leukocytes in five high-power fields may increase the sensitivity of frozen section, with minimal impact on specificity.

Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects

PubMed Central

Kriebardis, Anastasios G.; Antonelou, Marianna H.; Georgatzakou, Hara T.; Tzounakas, Vassilis L.; Stamoulis, Konstantinos E.; Papassideri, Issidora S.

2016-01-01

Background Extracellular vesicles or microparticles exhibiting procoagulant and thrombogenic activity may contribute to the haemostatic potential of fresh frozen plasma. Materials and methods Fresh frozen plasma was prepared from platelet-rich plasma at 20 °C (Group-1 donors) or directly from whole blood at 4 °C (Group-2 donors). Each unit was aseptically divided into three parts, stored frozen for specific periods of time, and analysed by flow cytometry for procoagulant activity immediately after thaw or following post-thaw storage for 24 h at 4 °C. Donors’ haematologic, biochemical and life-style profiles as well as circulating microparticles were analysed in parallel. Results Circulating microparticles exhibited a considerable interdonor but not intergroup variation. Fresh frozen plasma units were enriched in microparticles compared to plasma in vivo. Duration of storage significantly affected platelet- and red cell-derived microparticles. Fresh frozen plasma prepared directly from whole blood contained more residual platelets and more platelet-derived microparticles compared to fresh frozen plasma prepared from platelet-rich plasma. Consequently, there was a statistically significant difference in total, platelet- and red cell-derived microparticles between the two preparation protocols over storage time in the freezer. Preservation of the thawed units for 24 h at 4 °C did not significantly alter microparticle accumulation. Microparticle accumulation and anti-oxidant capacity of fresh frozen plasma was positively or negatively correlated, respectively, with the level of circulating microparticles in individual donors. Discussion The preparation protocol and the duration of storage in the freezer, independently and in combination, influenced the accumulation of microparticles in fresh frozen plasma units. In contrast, storage of thawed units for 24 h at 4 °C had no significant effect on the concentration of microparticles. PMID:27136430

Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects.

PubMed

Kriebardis, Anastasios G; Antonelou, Marianna H; Georgatzakou, Hara T; Tzounakas, Vassilis L; Stamoulis, Konstantinos E; Papassideri, Issidora S

2016-05-01

Extracellular vesicles or microparticles exhibiting procoagulant and thrombogenic activity may contribute to the haemostatic potential of fresh frozen plasma. Fresh frozen plasma was prepared from platelet-rich plasma at 20 °C (Group-1 donors) or directly from whole blood at 4 °C (Group-2 donors). Each unit was aseptically divided into three parts, stored frozen for specific periods of time, and analysed by flow cytometry for procoagulant activity immediately after thaw or following post-thaw storage for 24 h at 4 °C. Donors' haematologic, biochemical and life-style profiles as well as circulating microparticles were analysed in parallel. Circulating microparticles exhibited a considerable interdonor but not intergroup variation. Fresh frozen plasma units were enriched in microparticles compared to plasma in vivo. Duration of storage significantly affected platelet- and red cell-derived microparticles. Fresh frozen plasma prepared directly from whole blood contained more residual platelets and more platelet-derived microparticles compared to fresh frozen plasma prepared from platelet-rich plasma. Consequently, there was a statistically significant difference in total, platelet- and red cell-derived microparticles between the two preparation protocols over storage time in the freezer. Preservation of the thawed units for 24 h at 4 °C did not significantly alter microparticle accumulation. Microparticle accumulation and anti-oxidant capacity of fresh frozen plasma was positively or negatively correlated, respectively, with the level of circulating microparticles in individual donors. The preparation protocol and the duration of storage in the freezer, independently and in combination, influenced the accumulation of microparticles in fresh frozen plasma units. In contrast, storage of thawed units for 24 h at 4 °C had no significant effect on the concentration of microparticles.

Use of frozen section in genitourinary pathology.

PubMed

Shen, Steven S; Truong, Luan D; Ro, Jae Y; Ayala, Alberto G

2012-08-01

Frozen section diagnosis provides critical information for immediate surgical management decision making. Over the last several years, there have been some significant advances in treatment of genitourinary cancer, particularly with regard to surgical techniques. These changes in turn impact the type and frequency of intraoperative frozen section requests. In this review, we describe the main indications and diagnostic challenges of frozen section diagnosis during surgeries of each genitourinary organ system including prostate, kidney, bladder, testis, and penis. The pitfalls and approaches to different diagnostic situations are discussed. It is also stressed that pathologists must not only be familiar with the histological diagnosis, but also understand the limitations of frozen section diagnosis and communicate with urologists during the intraoperative treatment decision making process.

The Construction of an Embankment with Frozen Soil.

DTIC Science & Technology

1980-05-01

Idewlrty by block numlbe) Inls paper presents the construction procedure, data and analysis from an experimental field program to determine the rippability ...finite element computer solution. Also the frozen chunks, produced in the ripping operation, were analyzed to determine the rippability of the frozen

The effects of soy on freezable bread dough: a magnetic resonance study.

PubMed

Simmons, Amber L; Vodovotz, Yael

2012-11-15

Hygroscopic soy ingredients were hypothesised to slow the rate of water migration in unleavened bread dough during frozen storage. Thawed soy (18% dry weight) and wheat dough samples were assessed using non-destructive nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) for up to 8 wks frozen storage time. MRI suggested a spatially homogeneous, net increase in proton mobility with frozen storage and, with solution state proton NMR, distinct "free" and "bound" states were discerned. T(2) relaxation times of the majority proton population suggested increased mobility with frozen storage time, and statistical difference from the fresh sample was seen later for the soy samples than the wheat samples. As seen by (13)C-solid state NMR, the crystallinity of the starch was not affected by either soy addition or frozen storage. In conclusion, addition of soy to bakery products led to slightly enhanced preservation of "fresh" characteristics of the dough during frozen storage. Copyright © 2012 Elsevier Ltd. All rights reserved.

Long-term red meat preservation using chilled and frozen storage combinations: A review.

PubMed

Coombs, Cassius E O; Holman, Benjamin W B; Friend, Michael A; Hopkins, David L

2017-03-01

This paper reviews current literature relating to the effects of chilled and frozen storage on the quality characteristics of red meat (lamb and beef). These characteristics include tenderness (shear force), juiciness (fluid losses), flavour (lipid oxidation), colour and microbial loading. Research suggests that chilled storage of red meat can improve certain properties, such as shear force and species-specific flavour, to threshold levels before the effects on flavour and colour become deleterious, and key microbial species proliferate to spoilage levels. For frozen red meat, the negative effects upon quality traits are prevented when stored for shorter durations, although chilled storage conditions prior to freezing and retail display post-thawing can both positively and negatively affect these traits. Here, we review the effects of different chilled, frozen and combined chilled and frozen storage practices (particularly the chilled-then-frozen combination) on meat quality and spoilage traits, in order to contribute to superior management of these traits during product distribution. Crown Copyright © 2016. Published by Elsevier Ltd. All rights reserved.

Effect of fructo-oligosaccharide and isomalto-oligosaccharide addition on baking quality of frozen dough.

PubMed

Park, Eun Young; Jang, Sung-Bum; Lim, Seung-Taik

2016-12-15

The baking quality of frozen doughs containing different levels of fructo-oligosaccharides (FO) or isomalto-oligosaccharides (IMO) (3-9%, w/w flour), and stored for 0-8weeks at -18°C, was examined. The addition of FO or IMO increased the proof volume of the dough and the loaf volume of bread prepared from frozen dough. A 6% addition of FO or IMO was optimum, giving the highest proof volume and bread loaf volume, but a higher concentration than 6% induced low baking quality including lower proof volume and bread loaf volume. The bread crumb was moister and softer after the addition of FO or IMO before, and even after, frozen storage. Darker crumb colour was observed in the bread after the addition of FO or IMO. The oligosaccharides added to the frozen dough were effective in improving the quality of bread made from frozen dough, except for resulting in a darker bread crumb. Copyright © 2016. Published by Elsevier Ltd.

Range of motion of diabetic frozen shoulder recovers to the contralateral level

PubMed Central

Vastamäki, Heidi; Ristolainen, Leena

2016-01-01

Objective To determine whether frozen shoulder heals equally well in patients with and without diabetes and whether dependency on insulin affects the outcome. Methods We retrospectively examined 178 patients with idiopathic frozen shoulder; 27 patients had diabetes. We evaluated range of motion, pain, and functional results. The mean follow-up was 9.7 years (SD, 7.1 years). Results In the presence of frozen shoulder, range of motion did not differ between patients with and without diabetes. At follow-up, range of motion in all directions of both the affected and unaffected shoulders of patients with diabetes was inferior to that of patients without diabetes. Among patients with diabetes, range of motion of the once-frozen shoulder reached the level of the unaffected shoulder. Patients with and without diabetes experienced similar pain except during exertion. The Constant–Murley score was not significantly different between the two groups, and insulin dependency did not lead to worse outcomes. Conclusion Frozen shoulder heals well in patients with diabetes. PMID:27856934

Effect of freezing on electrical properties and quality of thawed chicken breast meat

PubMed Central

Wei, Ran; Wang, Peng; Han, Minyi; Chen, Tianhao; Xu, Xinglian; Zhou, Guanghong

2017-01-01

Objective The objective of this research was to study the electrical properties and quality of frozen-thawed chicken breast meat and to investigate the relationship between these parameters at different times of frozen storage. Methods Thawed samples of chicken breast muscles were evaluated after being kept in frozen storage at −18°C for different periods of time (1, 2, 3, 4, 5, 6, 7, and 8 months). Results The results showed that water-holding capacity (WHC) and protein solubility decreased while thiobarbituric acid-reactive substances content increased with increasing storage time. The impedance module of samples decreased during 8-month frozen storage. Pearson correlation coefficients showed that the impedance change ratio (Q value) was significantly (p<0.05) related to pH, color, WHC, lipid oxidation and protein solubility, indicating a good relationship between the electrical properties and qualities of frozen-thawed chicken breast meat. Conclusion Impedance measurement has a potential to assess the quality of frozen chicken meat combining with quality indices. PMID:27554358

Sensory evaluation ratings and melting characteristics show that okra gum is an acceptable milk-fat ingredient substitute in chocolate frozen dairy dessert.

PubMed

Romanchik-Cerpovicz, Joelle E; Costantino, Amanda C; Gunn, Laura H

2006-04-01

Reducing dietary fat intake may lower the risk of developing coronary heart disease. This study examined the feasibility of substituting okra gum for 25%, 50%, 75%, or 100% milk fat in frozen chocolate dairy dessert. Fifty-six consumers evaluated the frozen dairy desserts using a hedonic scale. Consumers rated color, smell, texture, flavor, aftertaste, and overall acceptability characteristics of all products as acceptable. All ratings were similar among the products except for the aftertaste rating, which was significantly lower for chocolate frozen dairy dessert containing 100% milk-fat replacement with okra gum compared with the control (0% milk-fat replacement) (P<0.05). Whereas melting points of all products were similar, melting rates slowed significantly as milk-fat replacement with okra gum increased, suggesting that okra gum may increase the stability of frozen dairy desserts (P<0.05). Overall, this study shows that okra gum is an acceptable milk-fat ingredient substitute in chocolate frozen dairy dessert.

Effects of soy hull pectin and insoluble fiber on physicochemical and oxidative characteristics of fresh and frozen/thawed beef patties.

PubMed

Kim, Hyun-Wook; Miller, Danika K; Lee, Yong Jae; Kim, Yuan H Brad

2016-07-01

The objective of this study was to determine the effects of pectin and insoluble fiber isolated from soy hulls on water-holding capacity (WHC), texture, color, and lipid oxidation of fresh and frozen/thawed beef patties. Beef patties were formulated with no dietary fiber (control), 1% soy hull pectin, insoluble fiber, or their mixture (1:1), respectively. The addition of soy hull pectin significantly decreased display weight loss and increased cook yield of both fresh and frozen/thawed beef patties. In addition, no significant difference in hardness between fresh and frozen/thawed beef patties was observed for all dietary fiber treatments. However, incorporation of insoluble soy hull fiber decreased color and lipid oxidation stabilities of both fresh and frozen/thawed beef patties. Our results indicate that the incorporation of soy hull pectin could be an effective non-meat ingredient to minimize water loss and hardness defects of frozen beef patties. Copyright © 2016 Elsevier Ltd. All rights reserved.

Radicals derived from acetaldehyde and vinyl alcohol.

PubMed

Estep, Marissa L; Morgan, W James; Winkles, Alexander T; Abbott, Adam S; Villegas-Escobar, Nery; Mullinax, J Wayne; Turner, Walter E; Wang, Xiao; Turney, Justin M; Schaefer, Henry F

2017-10-18

Vinyl alcohol and acetaldehyde are isoelectronic products of incomplete butanol combustion. Along with the radicals resulting from the removal of atomic hydrogen or the hydroxyl radical, these species are studied here using ab initio methods as complete as coupled cluster theory with single, double, triple, and perturbative quadruple excitations [CCSDT(Q)], with basis sets as large as cc-pV5Z. The relative energies provided herein are further refined by including corrections for relativistic effects, the frozen core approximation, and the Born-Oppenheimer approximation. The effects of anharmonic zero-point vibrational energies are also treated. The syn conformer of vinyl alcohol is predicted to be lower in energy than the anti conformer by 1.1 kcal mol -1 . The alcoholic hydrogen of syn-vinyl alcohol is found to be the easiest to remove, requiring 84.4 kcal mol -1 . Five other radicals are also carefully considered, with four conformers investigated for the 1-hydroxyvinyl radical. Beyond energetics, we have conducted an overhaul of the spectroscopic literature for these species. Our results also provide predictions for fundamental modes yet to be reported experimentally. To our knowledge, the ν 3 (3076 cm -1 ) and ν 4 (2999 cm -1 ) C-H stretches for syn-vinyl alcohol and all but one of the vibrational modes for anti-vinyl alcohol (ν 1 -ν 14 ) are yet to be observed experimentally. For the acetyl radical, ν 6 (1035 cm -1 ), ν 11 (944 cm -1 ), ν 12 (97 cm -1 ), and accounting for our changes to the assignment of the 1419.9 cm -1 experimental mode, ν 10 (1441 cm -1 ), are yet to be observed. We have predicted these unobserved fundamentals and reassigned the experimental 1419.9 cm -1 frequency in the acetyl radical to ν 4 rather than to ν 10 . Our work also strongly supports reassignment of the ν 10 and ν 11 fundamentals of the vinoxy radical. We suggest that the bands assigned to the overtones of these fundamentals were in fact combination bands. Our findings may be useful in constructing improved combustion models of butanol and in spectroscopically characterizing these molecules further.

Influence of freezing and storing cherry fruit on its nutritional value.

PubMed

Vasylyshyna, Elena

2016-01-01

Cherries are a valuable dietary raw material and possess medicinal properties. Considering the nutritional, medical and vitamin value of cherry fruits, the purpose of this research was to produce a scientific justification for preserving the quality of cherry fruits using different freezing methods. To do this, cherry fruits from the Lotovka (Cerasus vulgaris) variety were frozen in various ways: packed in polyethylene bags (control); previously suspended in a 20% sugar solution and packing frozen cherry in polyethylene bags; suspended in a 20% sugar solution with the addition of 4% ascorutin and frozen followed by pre-packaging in polyethylene bags; cherry fruits were frozen in a 20% sugar solution in plastic cups of 0.25 cm3; they were also frozen in a 20% sugar solution with the addition of 4% ascorutin in plastic cups. The frozen products were stored at a temperature not higher than -18°C for up to 6 months. Result. Studies have shown the appropriateness of freezing cherry fruits, particularly in a 20% sugar solution with the addition of 4% ascorutin. The advantages of these fruits are in ascorbic acid preservation in 1. nd. Cherries are a valuable dietary raw material and possess medicinal properties. Considering the nutritional, medical and vitamin value of cherry fruits, the purpose of this research was to produce a scientific justification for preserving the quality of cherry fruits using different freezing methods. Material and methods. To do this, cherry fruits from the Lotovka (Cerasus vulgaris) variety were frozen in various ways: packed in polyethylene bags (control); previously suspended in a 20% sugar solution and packing frozen cherry in polyethylene bags; suspended in a 20% sugar solution with the addition of 4% ascorutin and frozen followed by pre-packaging in polyethylene bags; cherry fruits were frozen in a 20% sugar solution in plastic cups of 0.25 cm3; they were also frozen in a 20% sugar solution with the addition of 4% ascorutin in plastic cups. The frozen products were stored at a temperature not higher than -18°C for up to 6 months. Result. Studies have shown the appropriateness of freezing cherry fruits, particularly in a 20% sugar solution with the addition of 4% ascorutin. The advantages of these fruits are in ascorbic acid preservation in 1.5 times and reduction of tanning and coloring substances only by 27%, soluble solids to 7%, sugars - 4%, acids - 12%, in tasting evaluation of 5 points. Frozen cherry fruits in a 20% sugar solution with the addition of 4% ascorutin can be used in dietary nutrition for patients with cardiovascular diseases.

Microphysical Characteristics of Tropical Updrafts in Clean Conditions.

NASA Astrophysics Data System (ADS)

Stith, Jeffrey L.; Haggerty, Julie A.; Heymsfield, Andrew; Grainger, Cedric A.

2004-05-01

The distributions of ice particles, precipitation embryos, and supercooled water are examined within updrafts in convective clouds in the Amazon and at Kwajalein, Marshall Islands, based on in situ measurements during two Tropical Rainfall Measuring Mission field campaigns. Composite vertical profiles of liquid water, small particle concentration, and updraft/downdraft magnitudes exhibit similar peak values for the two tropical regions. Updrafts were found to be favored locations for precipitation embryos in the form of liquid or frozen drizzle-sized droplets. Most updrafts glaciated rapidly, removing most of the liquid water between -5° and -17°C. However, occasional encounters with liquid water occurred in much colder updraft regions. The updraft magnitudes where liquid water was observed at cold (e.g., -16° to -19°C) temperatures do not appear to be stronger than updrafts without liquid water at similar temperatures, however. The concentrations of small spherical frozen particles in glaciated regions without liquid water are approximately one-half of the concentrations in regions containing liquid cloud droplets, suggesting that a substantial portion of the cloud droplets may be freezing at relatively warm temperatures. Further evidence for a possible new type of aggregate ice particle, a chain aggregate found at cloud midlevels, is given.

Physicochemical properties of natural phenolics from grapes and olive oil byproducts and their antioxidant activity in frozen horse mackerel fillets.

PubMed

Pazos, Manuel; Alonso, Ana; Fernández-Bolaños, Juan; Torres, Josep L; Medina, Isabel

2006-01-25

The reducing and chelating capacities and the affinity for the incorporation into the fish muscle of grape procyanidins, hydroxytyrosol, and propyl gallate were studied together with their antioxidant activity in frozen horse mackerel (Trauchurus trauchurus) fillets. Fillets were supplemented with phenolic antioxidants by (a) spraying an aqueous phenolic solution, (b) glazing with an aqueous phenolic solution, and (c) a previous washing of fillets with water plus spraying an aqueous phenolic solution. The effect of washing on the endogenous pro-oxidant/antioxidant balance of the fillets was also determined. All phenolic compounds were effective delaying lipid oxidation in the fish fillets. The order of antioxidant efficiency in spraying and glazing was propyl gallate > hydroxytyrosol > procyanidins, which was similar to the reducing power of these phenolics, but did not show any correlation with their chelating capacity and their affinity to the fish muscle. Washing the fillets with water prior to spraying phenols increased synergistically the antioxidant activity of grape procyanidins and changed the relative antioxidant efficiency to propyl gallate approximately procyanidins > hydroxytyrosol. This synergism may be a result of a better distribution of the procyanidins onto the fillet surface because of the residual water that remained on the fillets surface after washing.

An activity recognition model using inertial sensor nodes in a wireless sensor network for frozen shoulder rehabilitation exercises.

PubMed

Lin, Hsueh-Chun; Chiang, Shu-Yin; Lee, Kai; Kan, Yao-Chiang

2015-01-19

This paper proposes a model for recognizing motions performed during rehabilitation exercises for frozen shoulder conditions. The model consists of wearable wireless sensor network (WSN) inertial sensor nodes, which were developed for this study, and enables the ubiquitous measurement of bodily motions. The model employs the back propagation neural network (BPNN) algorithm to compute motion data that are formed in the WSN packets; herein, six types of rehabilitation exercises were recognized. The packets sent by each node are converted into six components of acceleration and angular velocity according to three axes. Motor features such as basic acceleration, angular velocity, and derivative tilt angle were input into the training procedure of the BPNN algorithm. In measurements of thirteen volunteers, the accelerations and included angles of nodes were adopted from possible features to demonstrate the procedure. Five exercises involving simple swinging and stretching movements were recognized with an accuracy of 85%-95%; however, the accuracy with which exercises entailing spiral rotations were recognized approximately 60%. Thus, a characteristic space and enveloped spectrum improving derivative features were suggested to enable identifying customized parameters. Finally, a real-time monitoring interface was developed for practical implementation. The proposed model can be applied in ubiquitous healthcare self-management to recognize rehabilitation exercises.

Terrestrial analogs of possible Martian habitats

NASA Astrophysics Data System (ADS)

Friedmann, E. I.

Four environmental factors are responsible for the apparent absence of life on or near the surface of Mars: radiation, reactive oxidants, aridity and low temperature. The three latter factors are also present in terrestrial environments that approximate, although do not reach, the intensity of Martian conditions. Nor do they occur together in the same environments, yet they allow studying the response of microorganisms separately to each of these environmental factors. 1. Most laboratory experiments on radiation "resistance" deal with the ability of microorganisms to repair (in a radiation-free environment) previously suffered radiation damage. Little is known on the response to continuous high radiation environments. 2. Mars-like soils with reactive oxidants have recently (2004) been discovered in the most arid regions of the Atacama Desert. 3. Extreme aridity (absence of liquid water) has been studied in the Negev, Gobi and other deserts. In these habitats the sole primary producer worldwide is the cyanobacterium Chroococcidiopsis. This organism tolerates total desiccation for decades and upon wetting it resumes full activity within a few minutes. However, it utilizes only liquid water, not water vapor from the atmosphere. Both heterotrophic and photosynthetic bacteria (primary producers) reach their limit of existence in the extreme arid core of the Atacama Desert, possibly the driest place on Earth. 4. Extreme cold, per se, is not harmful to life: organisms in frozen state can be preserved for very long times. On Earth, "psychrophiles" (cold adapted microorganisms) live in oceans and lakes, in thermally stable aquatic environments with temperature optima around +5o C, and are unable to tolerate temperatures above +15o C. Extreme cold conditions occur only in non-aquatic (terrestrial) environments. Here the limiting factor is not low temperature, but rather the lack of high temperature necessary to drive metabolic processes. Microorganisms of these habitats are not well-adapted psychrophiles but psychrotolerant mesophiles with temperature optima around +20o C. In the thermally unstable environment of cryptoendolithic microorganisms inside rocks of the Antarctic cold desert the yearly temperature fluctuates from -45o C to (exceptionally) +22o C. In the thermally stable permafrost (frozen soil) the temperature is ca. -10o C in Siberia and -20o C to -30o C in Antarctica. The above environments will be discussed to some detail accompanied by slides of landscapes, close-ups and micrographs. The relevance of information from terrestrial analogs to biology of Mars will be critically discussed.

Accounting for crustal magnetization in models of the core magnetic field

NASA Technical Reports Server (NTRS)

Jackson, Andrew

1990-01-01

The problem of determining the magnetic field originating in the earth's core in the presence of remanent and induced magnetization is considered. The effect of remanent magnetization in the crust on satellite measurements of the core magnetic field is investigated. The crust as a zero-mean stationary Gaussian random process is modelled using an idea proposed by Parker (1988). It is shown that the matrix of second-order statistics is proportional to the Gram matrix, which depends only on the inner-products of the appropriate Green's functions, and that at a typical satellite altitude of 400 km the data are correlated out to an angular separation of approximately 15 deg. Accurate and efficient means of calculating the matrix elements are given. It is shown that the variance of measurements of the radial component of a magnetic field due to the crust is expected to be approximately twice that in horizontal components.

Seismic performance and design of bridge foundations in liquefiable ground with a frozen crust : [summary].

DOT National Transportation Integrated Search

2012-12-01

Two major earthquakes in Alaska, namely 1964 Great Alaskan earthquake and 2002 Denali earthquake, occurred in winter season when the ground crust was frozen. : None of the then-existing foundation types was able to withstand the force from the frozen...

Seismic performance and design of bridge foundations in liquefiable ground with a frozen crust.

DOT National Transportation Integrated Search

2012-12-01

Two major earthquakes in Alaska, namely 1964 Great Alaskan earthquake and 2002 Denali earthquake, occurred in winter season when the ground crust was frozen. None of the then-existing foundation types was able to withstand the force from the frozen c...

Surfactant-based EOR mediated by naturally occurring microorganisms

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomas, C.P.; Duvall, M.L.; Robertson, E.P.

1993-11-01

Oil recovery experiments using Bacillus licheniformis JF-2 (ATCC 39307) and a sucrose-based nutrient were performed with Berea sandstone cores (permeability 0.084 to 0.503 [mu]m [85 to 510 md]). Oil recovery efficiencies for four different crude oils (0.9396 to 0.8343 g/cm[sup 3] [19.1 to 38.1 [degree] API]) varied from 2.8% to 42.6% of the waterflood residual oil. Microbial systems reduced interfacial tension (IFT) [approximately]20 mN/m [[approximately]20 dyne/cm] for all oils tested. After the microbial flood experimentation, organisms were distributed throughout the core, with most cells near the outlet.

Controlled release liquid dosage formulation

DOEpatents

Benton, Ben F.; Gardner, David L.

1989-01-01

A liquid dual coated dosage formulation sustained release pharmaceutic having substantial shelf life prior to ingestion is disclosed. A dual coating is applied over controlled release cores to form dosage forms and the coatings comprise fats melting at less than approximately 101.degree. F. overcoated with cellulose acetate phthalate or zein. The dual coated dosage forms are dispersed in a sugar based acidic liquid carrier such as high fructose corn syrup and display a shelf life of up to approximately at least 45 days while still retaining their release profiles following ingestion. Cellulose acetate phthalate coated dosage form cores can in addition be dispersed in aqueous liquids of pH <5.

Calibrating the photo-thermal response of magneto-fluorescent gold nanoshells.

PubMed

Biswal, Nrusingh C; Ayala-Orzoco, Ciceron; Halas, Naomi J; Joshi, Amit

2011-01-01

We report the photothermal response and Near Infrared (NIR) imaging sensitivities of magneto-fluorescent silica core gold nanocomplexes designed for molecular image guided thermal therapy of cancer. Approximately 160 nm Silica core gold nanoshells were designed to provide NIR fluorescent and Magnetic Resonance (MR) contrast by incorporating FDA approved dye indocyanine green (ICG) and iron-oxide within an outer silica epilayer. The imaging and therapeutic sensitivity, and the stability of fluorescence contrast for 12 microliters of suspension (containing approximately 7.9 × 10(8) or 1.3 femtoMole nanoshells) buried at depths of 2-8 mm in tissue mimicking scattering media is reported.

Comparison of dynamical approximation schemes for non-linear gravitational clustering

NASA Technical Reports Server (NTRS)

Melott, Adrian L.

1994-01-01

We have recently conducted a controlled comparison of a number of approximations for gravitational clustering against the same n-body simulations. These include ordinary linear perturbation theory (Eulerian), the adhesion approximation, the frozen-flow approximation, the Zel'dovich approximation (describable as first-order Lagrangian perturbation theory), and its second-order generalization. In the last two cases we also created new versions of approximation by truncation, i.e., smoothing the initial conditions by various smoothing window shapes and varying their sizes. The primary tool for comparing simulations to approximation schemes was crosscorrelation of the evolved mass density fields, testing the extent to which mass was moved to the right place. The Zel'dovich approximation, with initial convolution with a Gaussian e(exp -k(exp 2)/k(exp 2, sub G)) where k(sub G) is adjusted to be just into the nonlinear regime of the evolved model (details in text) worked extremely well. Its second-order generalization worked slightly better. All other schemes, including those proposed as generalizations of the Zel'dovich approximation created by adding forces, were in fact generally worse by this measure. By explicitly checking, we verified that the success of our best-choice was a result of the best treatment of the phases of nonlinear Fourier components. Of all schemes tested, the adhesion approximation produced the most accurate nonlinear power spectrum and density distribution, but its phase errors suggest mass condensations were moved to slightly the wrong location. Due to its better reproduction of the mass density distribution function and power spectrum, it might be preferred for some uses. We recommend either n-body simulations or our modified versions of the Zel'dovich approximation, depending upon the purpose. The theoretical implication is that pancaking is implicit in all cosmological gravitational clustering, at least from Gaussian initial conditions, even when subcondensations are present.

Structure and photoluminescence properties of TeO2-core/TiO2-shell nanowires

NASA Astrophysics Data System (ADS)

Park, Sunghoon; An, Soyeon; Lee, Chongmu

2013-12-01

TeO2-core/TiO2-shell nanowires were fabricated by thermal evaporation of Te powders and MOCVD of TiO2. The as-synthesized TeO2 nanowires showed a weak broad violet band centered at approximately 430 nm. The emission peak was shifted to a bluish violet region (∼455 nm) by the encapsulation of the nanowires with a TiO2 thin film. The intensity of the major emission from the core-shell nanowires showed strong dependence on the shell layer thickness. The strongest emission was obtained for the shell layer thickness of ∼15 nm and its intensity was approximately 80 times higher than that of the violet emission from the as-synthesized TeO2 nanowires. This enhancement in emission intensity is attributed to the subwavelength optical resonant cavity formation in the shell layer. The major emission intensity was enhanced further and blue-shifted by annealing, which might be attributed to the increase in the Ti interstitial and O vacancy concentrations in the TeO2 cores during annealing.

Simulation of cracking cores when molding piston components

NASA Astrophysics Data System (ADS)

Petrenko, Alena; Soukup, Josef

2014-08-01

The article deals with pistons casting made from aluminum alloy. Pistons are casting at steel mold with steel core. The casting is provided by gravity casting machine. The each machine is equipped by two metal molds, which are preheated above temperature 160 °C before use. The steel core is also preheated by flame. The metal molds and cores are heated up within the casting process. The temperature of the metal mold raise up to 200 °C and temperature of core is higher. The surface of the core is treated by nitration. The mold and core are cooled down by water during casting process. The core is overheated and its top part is finally cracked despite its intensive water-cooling. The life time cycle of the core is decreased to approximately 5 to 15 thousands casting, which is only 15 % of life time cycle of core for production of other pistons. The article presents the temperature analysis of the core.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Broda, Jill Terese

The neutron flux across the nuclear reactor core is of interest to reactor designers and others. The diffusion equation, an integro-differential equation in space and energy, is commonly used to determine the flux level. However, the solution of a simplified version of this equation when automated is very time consuming. Since the flux level changes with time, in general, this calculation must be made repeatedly. Therefore solution techniques that speed the calculation while maintaining accuracy are desirable. One factor that contributes to the solution time is the spatial flux shape approximation used. It is common practice to use the samemore » order flux shape approximation in each energy group even though this method may not be the most efficient. The one-dimensional, two-energy group diffusion equation was solved, for the node average flux and core k-effective, using two sets of spatial shape approximations for each of three reactor types. A fourth-order approximation in both energy groups forms the first set of approximations used. The second set used combines a second-order approximation with a fourth-order approximation in energy group two. Comparison of the results from the two approximation sets show that the use of a different order spatial flux shape approximation results in considerable loss in accuracy for the pressurized water reactor modeled. However, the loss in accuracy is small for the heavy water and graphite reactors modeled. The use of different order approximations in each energy group produces mixed results. Further investigation into the accuracy and computing time is required before any quantitative advantage of the use of the second-order approximation in energy group one and the fourth-order approximation in energy group two can be determined.« less

Road icing forecasting and detecting system

NASA Astrophysics Data System (ADS)

Xu, Hongke; Zheng, Jinnan; Li, Peiqi; Wang, Qiucai

2017-05-01

Regard for the facts that the low accuracy and low real-time of the artificial observation to determine the road icing condition, and it is difficult to forecast icing situation, according to the main factors influencing the road-icing, and the electrical characteristics reflected by the pavement ice layer, this paper presents an innovative system, that is, ice-forecasting of the highway's dangerous section. The system bases on road surface water salinity measurements and pavement temperature measurement to calculate the freezing point of water and temperature change trend, and then predicts the occurrence time of road icing; using capacitance measurements to verdict the road surface is frozen or not; This paper expounds the method of using single chip microcomputer as the core of the control system and described the business process of the system.

77 FR 20356 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Extension of Preliminary...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-04

... DEPARTMENT OF COMMERCE International Trade Administration [A-552-801] Certain Frozen Fish Fillets... frozen fish fillets (``fish fillets'') from the Socialist Republic of Vietnam (``Vietnam''). These... on fish fillets from Vietnam.\\1\\ On October 3, 2011 the Department published a notice of initiation...

Using Disney's "Frozen" to Motivate Mathematics: Bringing Fractals into the Classroom

ERIC Educational Resources Information Center

Piatek-Jimenez, Katrina; Phelps, Christine M.

2016-01-01

The movie "Frozen" took the world by storm and this global popularity of the movie and its music can be harnessed by teachers of mathematics. This article builds on the "frozen fractal" lyric from "Let It Go" to incorporate fractal geometry into primary mathematics classrooms.

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

21 CFR 146.146 - Frozen concentrated orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Fruit Juices and Beverages § 146.146 Frozen concentrated orange juice. (a) Frozen concentrated orange... sweetening ingredients specified in paragraph (b) of this section may be added to adjust the final... any added optional sweetening ingredients. The dilution ratio shall be not less than 3 plus 1. For the...

75 FR 22424 - Frozen Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-28

... Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam AGENCY: United States International Trade... antidumping duty orders on frozen warmwater shrimp from Brazil, China, India, Thailand, and Vietnam. SUMMARY... duty orders on frozen warmwater shrimp from Brazil, China, India, Thailand, and Vietnam would be likely...

78 FR 15691 - Certain Frozen Warmwater Shrimp From India; Preliminary Results of Antidumping Duty...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-12

... Shrimp From India; Preliminary Results of Antidumping Duty Administrative Review; 2011-2012 AGENCY... certain frozen warmwater shrimp (shrimp) from India. The review covers 195 producers/ exporters of the... of Antidumping Duty Changed Circumstances Review: Certain Frozen Warmwater Shrimp From India, 77 FR...

7 CFR 318.13-13 - Movement of frozen fruits and vegetables.

Code of Federal Regulations, 2010 CFR

2010-01-01

... HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE STATE OF HAWAII AND TERRITORIES QUARANTINE NOTICES Regulated Articles From Hawaii and the Territories § 318.13-13 Movement of frozen fruits and vegetables. Frozen fruits and vegetables may be certified for movement from Hawaii, Puerto Rico, Guam, the...

9 CFR 327.21 - Inspection procedures for chilled fresh and frozen boneless manufacturing meat.

Code of Federal Regulations, 2010 CFR

2010-01-01

... fresh and frozen boneless manufacturing meat. 327.21 Section 327.21 Animals and Animal Products FOOD... MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION IMPORTED PRODUCTS § 327.21 Inspection procedures for chilled fresh and frozen boneless manufacturing meat. (a) Definitions...

9 CFR 327.21 - Inspection procedures for chilled fresh and frozen boneless manufacturing meat.

Code of Federal Regulations, 2011 CFR

2011-01-01

... fresh and frozen boneless manufacturing meat. 327.21 Section 327.21 Animals and Animal Products FOOD... MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION IMPORTED PRODUCTS § 327.21 Inspection procedures for chilled fresh and frozen boneless manufacturing meat. (a) Definitions...

5 CFR 1653.3 - Processing retirement benefits court orders.

Code of Federal Regulations, 2010 CFR

2010-01-01

...): (1) The participant's account number or Social Security number (SSN); (2) The name and last known... not complete, the participant's account will be frozen. After the account is frozen, no withdrawal or..., and the account will remain frozen for loans and withdrawals. (ii) In the absence of proper...

Effects of Storage Temperature on the Quality of Frozen Squid

NASA Astrophysics Data System (ADS)

Kozima, Tsuneo; Ohtaka, Tateo

Squid ( Todarodes pacificus) , which is one of the main Japanzse coastal fish , was frozen under commercial condition after catch immediately and stored at -18 , -23 , -30 °C and 40 °C for 12 months. During storage the quality was measured by determining amount of free drip , water-holding capacity of muscle , weight ratio of cooking loss , histoligical feature of frozen and thawed muscle , and organoleptic test at regular intervals , each 2 months. Storage life of frozen squid was 12 months at below -18 °C.

The U.S. Navy’s Experience with Resuscitation of Wounded Servicemen in Vietnam Using Frozen Washed Red Blood Cells - 1966-1973; Developments from This Experience

DTIC Science & Technology

1982-02-18

resuscitatioh of wounded servicemen in Vietnam. Results of the U. S. Navy field-testing of :.....’ the first generation frozen blood bank system in...Vietnam between 1966 and 10968 .ir.. have demonstrated the feasibility of a frozen blood bank system to supplement the liquid blood bank system deployed...treatment prior to freeze-preservation.1 he second generation integrated liquid-frozen blood bank system has been field-tested "rely tthebe.Eilet

The “Ice Age” of Anatomy and Obstetrics:

PubMed Central

Al-Gailani, Salim

2016-01-01

summary In the late nineteenth century anatomists claimed a new technique—slicing frozen corpses into sections—translated the three-dimensional complexity of the human body into flat, visually striking, and unprecedentedly accurate images. Traditionally hostile to visual aids, elite anatomists controversially claimed frozen sections had replaced dissection as the “true anatomy.” Some obstetricians adopted frozen sectioning to challenge anatomists’ authority and reform how clinicians made and used pictures. To explain the successes and failures of the technique, this article reconstructs the debates through which practitioners learned to make and interpret, to promote or denigrate frozen sections in teaching and research. Focusing on Britain, the author shows that attempts to introduce frozen sectioning into anatomy and obstetrics shaped and were shaped by negotiations over the epistemological standing of hand and eye in medicine.

Freezing without Ice Crystal Damage: Semithin and Ultrathin Frozen Sections of Ethanol-Infiltrated Tissue for Microscopy, with Applications to Immunocytochemistry

NASA Astrophysics Data System (ADS)

Christensen, A. Kent; Lowry, Terry B.

1995-10-01

Ethanol (ethyl alcohol) has long been a standard reagent used in preparing tissues for light and electron microscopy. After fixation, tissues are usually dehydrated with ethanol before being embedded in paraffin or plastic. In this study we show that the ethanol-infiltrated tissue can be frozen and sectioned directly without embedding. When tissue impregnated with ethanol is cooled below about [minus sign]117°C with liquid nitrogen, the ethanol solidifies without appreciable crystallization. The frozen tissue can then be sectioned in a commercial cryoultramicrotome that is set at [minus sign]155 to [minus sign]170°C to produce semithin frozen sections (0.5 to 3 [mu]m thick) for light microscopy or ultrathin frozen sections (50 to 100 nm thick) for electron microscopy. Sections are picked up and mounted on glass slides or EM grids by means that are in current use for ice ultrathin frozen sectioning. Because there is no apparent freezing damage, the morphology in these ethanol frozen sections of unembedded tissue appears generally quite good, often resembling that obtained by conventional EM techniques. Examples are provided that illustrate the use of this material for immunocytochemistry at the light and electron microscope levels.

Clinical assessment, gross examination, frozen section of ovarian masses: do patients benefit?

PubMed

Ghaemmaghami, Fatemah; Fakour, Fereshteh; Karimi Zarchi, Mojgan; Behtash, Nadereh; Modares Gilani, Mitra; Mousavi, Azamsadat; Shariat, Mamak

2008-09-01

The overall risk of malignancy in ovarian neoplasm is 13% in premenopausal women and 45% in postmenopausal women. Differentiating benign and malignant disease with frozen section is possible during operation; however, information on patients' history, physical examination, paraclinical criteria (tumour markers, imaging) and gross examination of tumour can also be helpful in planing the surgery. This study was conducted on 150 women who underwent laparotomy due to adnexal mass between April 2003 and October 2005 at Vali-e-Asr Hospital, Tehran, Iran. Sensitivity and specificity of clinical assessment (history, tumour marker and imaging), gross examination and frozen section were calculated. Based on our findings frozen section had the highest sensitivity for diagnosing malignant tumour comparing with other methods of diagnosis (88.9%). Sensitivity was 71.3% for preoperative clinical examination, 83% for ultrasonography, 89.8% for CT scan, 70% for CA125 and 84.1% for gross examination, likewise the highest specificity was seen for frozen section (93.5%). This data confirm that frozen section diagnosis is a reliable method for the surgical management of patients with an ovarian mass, but history of disease, Para clinical criteria and gross examination can help to surgeon to perform on appropriate operation in the areas where frozen section is not possible.