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Sample records for fur seal callorhinus

  1. Salmonella meningoencephalomyelitis in a northern fur seal (Callorhinus ursinsus)

    USGS Publications Warehouse

    Stroud, R.K.; Roelke, M.E.

    1980-01-01

    Salmonella enteritidis was isolated from the brain of a neonatal northern fur seal (Callorhinus ursinus) with gross and microscopic lesions of meningoencephalomyelitis. Microscopic lesions in the liver and lung suggested septicemia.

  2. Organohalogen Contaminants and Vitamins in Northern Fur Seals (Callorhinus ursinus) Collected During Subsistence Hunts in Alaska.

    PubMed

    Reiner, Jessica L; Becker, Paul R; Gribble, Matthew O; Lynch, Jennifer M; Moors, Amanda J; Ness, Jennifer; Peterson, Danielle; Pugh, Rebecca S; Ragland, Tamika; Rimmer, Catherine; Rhoderick, Jody; Schantz, Michele M; Trevillian, Jennifer; Kucklick, John R

    2016-01-01

    During native subsistence hunts from 1987 to 2007, blubber and liver samples from 50 subadult male northern fur seals (Callorhinus ursinus) were collected on St. Paul Island, Alaska. Samples were analyzed for legacy persistent organic pollutants (POPs), recently phased-out/current-use POPs, and vitamins. The legacy POPs measured from blubber samples included polychlorinated biphenyl congeners, DDT (and its metabolites), chlorobenzenes, chlordanes, and mirex. Recently phased-out/current-use POPs included in the blubber analysis were the flame retardants, polybrominated diphenyl ethers, and hexabromocyclododecanes. The chemical surfactants, perfluorinated alkyl acids, and vitamins A and E were assessed in the liver samples. Overall, concentrations of legacy POPs are similar to levels seen in seal samples from other areas of the North Pacific Ocean and the Bering Sea. Statistically significant correlations were seen between compounds with similar functions (pesticides, flame retardants, vitamins). With sample collection spanning two decades, the temporal trends in the concentrations of POPs and vitamins were assessed. For these animals, the concentrations of the legacy POPs tend to decrease or stay the same with sampling year; however, the concentrations of the current-use POPs increased with sampling year. Vitamin concentrations tended to stay the same across the sampling years. With the population of northern fur seals from St. Paul Island on the decline, a detailed assessment of exposure to contaminants and the correlations with vitamins fills a critical gap for identifying potential population risk factors that might be associated with health effects.

  3. Organohalogen contaminants and vitamins in Northern Fur Seals (Callorhinus ursinus) collected during subsistence hunts in Alaska

    PubMed Central

    Reiner, Jessica L.; Becker, Paul R.; Gribble, Matthew O.; Lynch, Jennifer M.; Moors, Amanda J.; Ness, Jennifer; Peterson, Danielle; Pugh, Rebecca S.; Ragland, Tamika; Rimmer, Catherine; Rhoderick, Jody; Schantz, Michele M.; Trevillian, Jennifer; Kucklick, John R.

    2016-01-01

    During native subsistence hunts from 1987 to 2007, blubber and liver samples from 50 subadult male northern fur seals (Callorhinus ursinus) were collected on St. Paul Island, Alaska. Samples were analyzed for legacy persistent organic pollutants (POPs), recently phased out/current-use POPs, and vitamins. The legacy POPs measured from blubber samples included polychlorinated biphenyl congeners, dichlorodiphenyltrichloroethanes (DDT and metabolites), chlorobenzenes, chlordanes, and mirex. Recently phased-out/current-use POPs included in the blubber analysis were the flame retardants, polybrominated diphenyl ethers, and hexabromocyclododecanes. The chemical surfactants, perfluorinated alkyl acids and vitamins A and E were assessed in the liver samples. Overall, concentrations of legacy POPs are similar to levels seen in seal samples from other areas of the North Pacific Ocean and the Bering Sea. Statistically significant correlations were seen between compounds with similar functions (pesticides, flame retardants, vitamins). With sample collection spanning two decades, the temporal trends in the concentrations of POPs and vitamins were assessed. For these animals, the concentrations of the legacy POPs tend to decrease or stay the same with sampling year; however, the concentrations of the current-use POPs increased with sampling year. Vitamin concentrations tended to stay the same across the sampling years. With the population of northern fur seals from St. Paul Island on the decline, a detailed assessment of exposure to contaminants and the correlations with vitamins fills a critical gap for identifying potential population risk factors that might be associated with health effects. PMID:26142120

  4. Stable Isotope Models Predict Foraging Habitat of Northern Fur Seals (Callorhinus ursinus) in Alaska.

    PubMed

    Zeppelin, T K; Johnson, D S; Kuhn, C E; Iverson, S J; Ream, R R

    2015-01-01

    We developed models to predict foraging habitat of adult female northern fur seals (Callorhinus ursinus) using stable carbon (δ13C) and nitrogen (δ15N) isotope values from plasma and red blood cells. Binomial generalized linear mixed models were developed using blood isotope samples collected from 35 adult female fur seals on three breeding colonies in Alaska during July-October 2006. Satellite location and dive data were used to define habitat use in terms of the proportion of time spent or dives made in different oceanographic/bathymetric domains. For both plasma and red blood cells, the models accurately predicted habitat use for animals that foraged exclusively off or on the continental shelf. The models did not perform as well in predicting habitat use for animals that foraged in both on- and off-shelf habitat; however, sample sizes for these animals were small. Concurrently collected scat, fatty acid, and dive data confirmed that the foraging differences predicted by isotopes were associated with diet differences. Stable isotope samples, dive data, and GPS location data collected from an additional 15 females during August-October 2008 validated the effective use of the models across years. Little within year variation in habitat use was indicated from the comparison between stable isotope values from plasma (representing 1-2 weeks) and red blood cells (representing the prior few months). Constructing predictive models using stable isotopes provides an effective means to assess habitat use at the population level, is inexpensive, and can be applied to other marine predators.

  5. Stable Isotope Models Predict Foraging Habitat of Northern Fur Seals (Callorhinus ursinus) in Alaska

    PubMed Central

    Zeppelin, T. K.; Johnson, D. S.; Kuhn, C. E.; Iverson, S. J.; Ream, R. R.

    2015-01-01

    We developed models to predict foraging habitat of adult female northern fur seals (Callorhinus ursinus) using stable carbon (δ13C) and nitrogen (δ15N) isotope values from plasma and red blood cells. Binomial generalized linear mixed models were developed using blood isotope samples collected from 35 adult female fur seals on three breeding colonies in Alaska during July-October 2006. Satellite location and dive data were used to define habitat use in terms of the proportion of time spent or dives made in different oceanographic/bathymetric domains. For both plasma and red blood cells, the models accurately predicted habitat use for animals that foraged exclusively off or on the continental shelf. The models did not perform as well in predicting habitat use for animals that foraged in both on- and off-shelf habitat; however, sample sizes for these animals were small. Concurrently collected scat, fatty acid, and dive data confirmed that the foraging differences predicted by isotopes were associated with diet differences. Stable isotope samples, dive data, and GPS location data collected from an additional 15 females during August-October 2008 validated the effective use of the models across years. Little within year variation in habitat use was indicated from the comparison between stable isotope values from plasma (representing 1-2 weeks) and red blood cells (representing the prior few months). Constructing predictive models using stable isotopes provides an effective means to assess habitat use at the population level, is inexpensive, and can be applied to other marine predators. PMID:26030280

  6. The cestode community in northern fur seals (Callorhinus ursinus) on St. Paul Island, Alaska

    PubMed Central

    Kuzmina, Tetiana A.; Hernández-Orts, Jesús S.; Lyons, Eugene T.; Spraker, Terry R.; Kornyushyn, Vadym V.; Kuchta, Roman

    2015-01-01

    The diversity and ecology of cestodes from the northern fur seals, Callorhinus ursinus (NFS), were examined using newly collected material from 756 humanely harvested subadult males between 2011 and 2014. NFSs were collected from five different haul-outs on St. Paul Island, Alaska. A total of 14,660 tapeworms were collected with a prevalence of 98.5% and intensity up to 107 cestodes per host (mean intensity 19.7 ± 16.5 SD). Three species of tapeworms were found: Adenocephalus pacificus (Diphyllobothriidea) was the most prevalent (prevalence 97.4%), followed by Diplogonoporus tetrapterus (49.7%), and 5 immature specimens of Anophryocephalus cf. ochotensis (Tetrabothriidea) (0.5%). Most of the cestodes found in the NFS were immature (69.7%). However, only 0.9% of cestodes were in larval (plerocercoid) stages. The species composition, prevalence and intensity of cestodes from these NFSs were not statistically different between the five separate haul-outs. Significant increases in the intensity of NFS infections were observed during the study period. PMID:26101743

  7. Isotopic and genetic insights into the persistence of the northern fur seal (Callorhinus ursinus) (Invited)

    NASA Astrophysics Data System (ADS)

    Koch, P. L.; Hadly, E. A.; Pinsky, M. L.; Newsome, S. D.

    2010-12-01

    What factors allow some species to survive in the face of climate change, disease, or anthropogenic disturbance? How do species shift their geographic distributions in the face of such challenges? These pressing questions in ecology and conservation biology are difficult to answer when looking solely at modern populations or the recent historical record. We explore these questions through analysis of DNA and the isotopic composition of modern and ancient northern fur seals (NFS, Callorhinus ursinus). The NFS is an eared seal (otariid) that ranges along the north Pacific, where it breeds on offshore islands; by far the largest modern rookeries are on the Pribilof Islands in the Bering Sea. The species shows a high degree of philopatry, and females feed while nursing, wean pups at 4 months, and spend the rest of the year foraging far offshore further south. Archaelogical study reveals that Holocene NFS had numerous breeding colonies from the Channel Islands to the Aleutians. Temperate latitude colonies collapsed in the late Holocene in response to hunting pressures and perhaps, environmental change. The species has recolonized parts of its former range since the 1960s. Despite facing similar threats, other marine mammals have failed to rebound (e.g., Guadalupe fur seals) or have exceptionally low genetic diversity indicating recent and prolonged bottlenecks (e.g., northern elephant seals). Isotopic analyses of sub-fossil growth series indicate that extirpated mid-latitude colonies weaned much later (≥12 months), like all other otariid species that breed at temperate latitudes. As a result, females were tied to rookery sites year-round and had a much-reduced migratory range relative to modern NFS females breeding in the Bering Sea, a result also supported by isotopic analyses. Serial coalescent simulations of ancient and modern DNA reveals that exceptionally high migration rates and Arctic refugia provided resilience to NFS. These traits allowed the species to

  8. Dental and Temporomandibular Joint Pathology of the Northern Fur Seal (Callorhinus ursinus).

    PubMed

    Aalderink, M T; Nguyen, H P; Kass, P H; Arzi, B; Verstraete, F J M

    2015-05-01

    Skulls from 145 northern fur seals (Callorhinus ursinus) were examined macroscopically according to predefined criteria. The museum specimens were acquired from strandings along the west coast of the USA between 1896 and 2008. Seventy-one skulls (49.0%) were from male animals, 56 (38.6%) from female animals and 18 (12.4%) from animals of unknown sex. Their age varied from juvenile to adult, with 58 adult animals (40.0%) and 87 juvenile animals (60.0%). The majority of teeth were available for examination (95.1%); 3.4% of teeth were artefactually absent, 0.8% were deemed absent due to acquired tooth loss and 0.6% were deemed congenitally absent. Males were no more likely than females to have either acquired tooth loss (P = 0.054) or congenitally absent teeth (P = 0.919). Adults had significantly more acquired tooth loss than juveniles (P = 0.0099). Malformations were seen in 11 teeth (0.2% of all 4,699 teeth available for examination). Two roots, instead of the typical one root, were found on 14 teeth (0.3%). Supernumerary teeth were associated with 14 normal teeth (0.3%) in eight specimens (5.5% of the total number of specimens). A total of 22 persistent deciduous teeth were found, 19 of which were associated with the maxillary canine teeth. Attrition/abrasion was seen on 194 teeth (3.9%); the canine teeth were most often affected, accounting for 39.7% of all abraded teeth. Adults were found to have a greater prevalence of abraded teeth than juveniles (P <0.0001). No significant difference was found in the appearance of attrition/abrasion between males and females (P = 0.072). Tooth fractures were found in 24 specimens (16.6%), affecting a total of 54 teeth (1.1%). Periapical lesions were found in two skulls (1.4%). None of the specimens showed signs of enamel hypoplasia. About a fifth (18.6%) of alveoli, either with or without teeth, showed signs of alveolar bony changes consistent with periodontitis. A total of 108 specimens (74.5%) had at least one tooth

  9. Apoptosis in normal and Coxiella burnetii-infected placentas from Alaskan northern fur seals (Callorhinus ursinus).

    PubMed

    Myers, E; Ehrhart, E J; Charles, B; Spraker, T; Gelatt, T; Duncan, C

    2013-07-01

    In 2010, Coxiella burnetii was identified in 75% of northern fur seal placentas from a single rookery in Alaska, but nothing was known about the significance of this organism in the population. Although many infectious organisms cause increased cell death, C. burnetii has been shown to suppress apoptosis of the host macrophages as an intracellular survival mechanism. To determine if infection induces a similar functional change in the placenta, immunohistochemistry for antibodies to cleaved caspase-3 (activated caspase-3) and the (TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) technique were used to compare the amount of placental apoptosis in infected and noninfected placentas. There was a statistically significant difference in the frequency of apoptotic cells between infected and uninfected placentas, with more apoptosis identified in the uninfected placentas. This finding suggests that the survival mechanism of C. burnetii in host macrophages to reduce apoptosis may also be utilized in trophoblasts. The significance of decreased trophoblastic apoptosis for the northern fur seal fetus requires further investigation.

  10. Update on the prevalence of the hookworm, Uncinaria lucasi, in northern fur seals (Callorhinus ursinus) on St. Paul Island, Alaska, 2011.

    PubMed

    Lyons, Eugene T; Kuzmina, Tetiana A; Tolliver, Sharon C; Spraker, Terry R

    2012-09-01

    Prevalence of hookworms (Uncinaria lucasi Stiles, 1901) was determined in the northern fur seal (Callorhinus ursinus Linnaeus, 1758) on St. Paul Island (SPI), Alaska in July and August, 2011. Three of 61 (4.9%) dead pups harbored 1 to 13 adult hookworms each in their intestines. Parasitic larvae (L(3)) of hookworms were recovered from the blubber of 4 of 133 (3%) of subadult males (SAMs) examined. One parasitic L(3) was detected from each infected SAM. Adult U. lucasi (n = 3) were found in the intestine of 1 of 105 SAMs examined (0.95%). This is the first documented finding of adult U. lucasi in SAMs of the northern fur seals. Continued low prevalence of hookworms the last several years parallels the tremendous decline in the number of fur seals on SPI over a similar time period.

  11. Isolation of San Miguel Sea Lion Virus from Samples of an animal food product produced from northern fur seal (Callorhinus ursinus) carcasses.

    PubMed

    Sawyer, J C; Madin, S H; Skilling, D E

    1978-01-01

    A virus was isolated from California sea lions (Zalophus californianus californianus) and northern fur seals (Callorhinus ursinus) in 1972. It was later named San Miguel sea lion virus (SMSV). State and federal livestock disease control agencies became concerned, because SMSV was found to be indistinguishable from vesicular exanthema of swine virus and to cause (in laboratory trials) clinical signs in swine similar to those produced by vesicular exanthema of swine virus. Ground carcasses of northern fur seals, salvaged after harvesting pelts, are fed to mink on ranches in the United States. Domestic swine are kept on some of these same ranches. Samples withheld from lots of this seal carcass mink food were found to contain SMSV (serotype 5) in titers of 10(6.1) and 10(6.8) tissue culture infective doses.

  12. Short-term episodes of imposed fasting have a greater effect on young northern fur seals (Callorhinus ursinus) in summer than in winter.

    PubMed

    Rosen, David A S; Volpov, Beth L; Trites, Andrew W

    2014-01-01

    An unexpected shortage of food may affect wildlife in a different way depending on the time of year when it occurs. We imposed 48 h fasts on six female northern fur seals (Callorhinus ursinus; ages 6-24 months) to identify times of year when they might be particularly sensitive to interruptions in food supply. We monitored changes in their resting metabolic rates and their metabolic response to thermal challenges, and also examined potential bioenergetic causes for seasonal differences in body mass loss. The pre-fast metabolism of the fur seals while in ambient air or submerged in water at 4°C was higher during summer (June to Sepember) than winter (November to March), and submergence did not significantly increase metabolism, indicating a lack of additional thermoregulatory costs. There was no evidence of metabolic depression following the fasting periods, nor did metabolism increase during the post-fast thermal challenge, suggesting that mass loss did not negatively impact thermoregulatory capacity. However, the fur seals lost mass at greater rates while fasting during the summer months, when metabolism is normally high to facilitate faster growth rates (which would ordinarily have been supported by higher food intake levels). Our findings suggest that summer is a more critical time of year than winter for young northern fur seals to obtain adequate nutrition.

  13. A NOVEL GAMMAHERPESVIRUS IN NORTHERN FUR SEALS (CALLORHINUS URSINUS) IS CLOSELY RELATED TO THE CALIFORNIA SEA LION (ZALOPHUS CALIFORNIANUS) CARCINOMA-ASSOCIATED OTARINE HERPESVIRUS-1.

    PubMed

    Cortés-Hinojosa, Galaxia; Gulland, Frances M D; DeLong, Robert; Gelatt, Tom; Archer, Linda; Wellehan, James F X

    2016-01-01

    Otarine herpesvirus 1 (OtHV1) is strongly associated with California sea lion (CSL, Zalophus californianus) urogenital carcinoma, the most common cancer documented in marine mammals. In addition to CSL, OtHV1 has also been found in association with carcinoma in South American fur seals (Arctocephalus australis), demonstrating it can infect related species. Northern fur seals (NFS, Callorhinus ursinus) are sympatric with CSL, and copulation between these species has been observed; yet, there are no reports of urogenital carcinoma in NFS. We describe a new Otarine herpesvirus found in vaginal swabs from NFS, herein called OtHV4. Partial sequencing of the polymerase gene and the glycoprotein B gene revealed OtHV4 is closely related to OtHV1, with 95% homology in the region of polymerase sequenced, and phylogenetic analyses demonstrate that they are sister taxa. An OtHV4-specific hydrolysis probe quantitative PCR was developed and validated, and its use on vaginal swabs revealed 16 of 50 (32%) wild adult female NFS were positive for OtHV4. The identification of a virus highly similar to the carcinoma-associated OtHV1 in a sympatric species without carcinoma suggests that comparative genomics of OtHV1 and OtHV4 may identify candidate viral oncogenes.

  14. Fortuitous encounters between seagliders and adult female northern fur seals (Callorhinus ursinus) off the Washington (USA) coast: upper ocean variability and links to top predator behavior.

    PubMed

    Pelland, Noel A; Sterling, Jeremy T; Lea, Mary-Anne; Bond, Nicholas A; Ream, Rolf R; Lee, Craig M; Eriksen, Charles C

    2014-01-01

    Behavioral responses by top marine predators to oceanographic features such as eddies, river plumes, storms, and coastal topography suggest that biophysical interactions in these zones affect predators' prey, foraging behaviors, and potentially fitness. However, examining these pathways is challenged by the obstacles inherent in obtaining simultaneous observations of surface and subsurface environmental fields and predator behavior. In this study, migratory movements and, in some cases, diving behavior of 40 adult female northern fur seals (NFS; Callorhinus ursinus) were quantified across their range and compared to remotely-sensed environmental data in the Gulf of Alaska and California Current ecosystems, with a particular focus off the coast of Washington State (USA)--a known foraging ground for adult female NFS and where autonomous glider sampling allowed opportunistic comparison of seal behavior to subsurface biophysical measurements. The results show that in these ecosystems, adult female habitat utilization was concentrated near prominent coastal topographic, riverine, or inlet features and within 200 km of the continental shelf break. Seal dive depths, in most ecosystems, were moderated by surface light level (solar or lunar), mirroring known behaviors of diel vertically-migrating prey. However, seal dives differed in the California Current ecosystem due to a shift to more daytime diving concentrated at or below the surface mixed layer base. Seal movement models indicate behavioral responses to season, ecosystem, and surface wind speeds; individuals also responded to mesoscale eddies, jets, and the Columbia River plume. Foraging within small scale surface features is consistent with utilization of the inner coastal transition zone and habitats near coastal capes, which are known eddy and filament generation sites. These results contribute to our knowledge of NFS migratory patterns by demonstrating surface and subsurface behavioral responses to a spatially

  15. Fortuitous Encounters between Seagliders and Adult Female Northern Fur Seals (Callorhinus ursinus) off the Washington (USA) Coast: Upper Ocean Variability and Links to Top Predator Behavior

    PubMed Central

    Pelland, Noel A.; Sterling, Jeremy T.; Lea, Mary-Anne; Bond, Nicholas A.; Ream, Rolf R.; Lee, Craig M.; Eriksen, Charles C.

    2014-01-01

    Behavioral responses by top marine predators to oceanographic features such as eddies, river plumes, storms, and coastal topography suggest that biophysical interactions in these zones affect predators' prey, foraging behaviors, and potentially fitness. However, examining these pathways is challenged by the obstacles inherent in obtaining simultaneous observations of surface and subsurface environmental fields and predator behavior. In this study, migratory movements and, in some cases, diving behavior of 40 adult female northern fur seals (NFS; Callorhinus ursinus) were quantified across their range and compared to remotely-sensed environmental data in the Gulf of Alaska and California Current ecosystems, with a particular focus off the coast of Washington State (USA) – a known foraging ground for adult female NFS and where autonomous glider sampling allowed opportunistic comparison of seal behavior to subsurface biophysical measurements. The results show that in these ecosystems, adult female habitat utilization was concentrated near prominent coastal topographic, riverine, or inlet features and within 200 km of the continental shelf break. Seal dive depths, in most ecosystems, were moderated by surface light level (solar or lunar), mirroring known behaviors of diel vertically-migrating prey. However, seal dives differed in the California Current ecosystem due to a shift to more daytime diving concentrated at or below the surface mixed layer base. Seal movement models indicate behavioral responses to season, ecosystem, and surface wind speeds; individuals also responded to mesoscale eddies, jets, and the Columbia River plume. Foraging within small scale surface features is consistent with utilization of the inner coastal transition zone and habitats near coastal capes, which are known eddy and filament generation sites. These results contribute to our knowledge of NFS migratory patterns by demonstrating surface and subsurface behavioral responses to a spatially

  16. The sun, moon, wind, and biological imperative-shaping contrasting wintertime migration and foraging strategies of adult male and female northern fur seals (Callorhinus ursinus).

    PubMed

    Sterling, Jeremy T; Springer, Alan M; Iverson, Sara J; Johnson, Shawn P; Pelland, Noel A; Johnson, Devin S; Lea, Mary-Anne; Bond, Nicholas A

    2014-01-01

    Adult male and female northern fur seals (Callorhinus ursinus) are sexually segregated in different regions of the North Pacific Ocean and Bering Sea during their winter migration. Explanations for this involve interplay between physiology, predator-prey dynamics, and ecosystem characteristics, however possible mechanisms lack empirical support. To investigate factors influencing the winter ecology of both sexes, we deployed five satellite-linked conductivity, temperature, and depth data loggers on adult males, and six satellite-linked depth data loggers and four satellite transmitters on adult females from St. Paul Island (Bering Sea, Alaska, USA) in October 2009. Males and females migrated to different regions of the North Pacific Ocean: males wintered in the Bering Sea and northern North Pacific Ocean, while females migrated to the Gulf of Alaska and California Current. Horizontal and vertical movement behaviors of both sexes were influenced by wind speed, season, light (sun and moon), and the ecosystem they occupied, although the expression of the behaviors differed between sexes. Male dive depths were aligned with the depth of the mixed layer during daylight periods and we suspect this was the case for females upon their arrival to the California Current. We suggest that females, because of their smaller size and physiological limitations, must avoid severe winters typical of the northern North Pacific Ocean and Bering Sea and migrate long distances to areas of more benign environmental conditions and where prey is shallower and more accessible. In contrast, males can better tolerate often extreme winter ocean conditions and exploit prey at depth because of their greater size and physiological capabilities. We believe these contrasting winter behaviors 1) are a consequence of evolutionary selection for large size in males, important to the acquisition and defense of territories against rivals during the breeding season, and 2) ease environmental

  17. The Sun, Moon, Wind, and Biological Imperative–Shaping Contrasting Wintertime Migration and Foraging Strategies of Adult Male and Female Northern Fur Seals (Callorhinus ursinus)

    PubMed Central

    Sterling, Jeremy T; Springer, Alan M.; Iverson, Sara J.; Johnson, Shawn P.; Pelland, Noel A.; Johnson, Devin S.; Lea, Mary-Anne; Bond, Nicholas A.

    2014-01-01

    Adult male and female northern fur seals (Callorhinus ursinus) are sexually segregated in different regions of the North Pacific Ocean and Bering Sea during their winter migration. Explanations for this involve interplay between physiology, predator-prey dynamics, and ecosystem characteristics, however possible mechanisms lack empirical support. To investigate factors influencing the winter ecology of both sexes, we deployed five satellite-linked conductivity, temperature, and depth data loggers on adult males, and six satellite-linked depth data loggers and four satellite transmitters on adult females from St. Paul Island (Bering Sea, Alaska, USA) in October 2009. Males and females migrated to different regions of the North Pacific Ocean: males wintered in the Bering Sea and northern North Pacific Ocean, while females migrated to the Gulf of Alaska and California Current. Horizontal and vertical movement behaviors of both sexes were influenced by wind speed, season, light (sun and moon), and the ecosystem they occupied, although the expression of the behaviors differed between sexes. Male dive depths were aligned with the depth of the mixed layer during daylight periods and we suspect this was the case for females upon their arrival to the California Current. We suggest that females, because of their smaller size and physiological limitations, must avoid severe winters typical of the northern North Pacific Ocean and Bering Sea and migrate long distances to areas of more benign environmental conditions and where prey is shallower and more accessible. In contrast, males can better tolerate often extreme winter ocean conditions and exploit prey at depth because of their greater size and physiological capabilities. We believe these contrasting winter behaviors 1) are a consequence of evolutionary selection for large size in males, important to the acquisition and defense of territories against rivals during the breeding season, and 2) ease environmental

  18. Current prevalence of adult Uncinaria spp. in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, California, with notes on the biology of these hookworms.

    PubMed

    Lyons, E T; Melin, S R; DeLong, R L; Orr, A J; Gulland, F M; Tolliver, S C

    2001-06-28

    A prevalence survey for hookworms (Uncinaria spp.) was done in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, CA, in 2000. Intestines of dead pups were examined for adult hookworms in July. These parasites were found in 95% of 20 fur seal pups and 100% of 31 sea lion pups. The number of hookworms varied from 4 to 2142 (mean = 760) in fur seal pups and from 20 to 2634 (mean = 612) in sea lion pups. A direct relationship was evident between body condition and number of hookworms in the pups; that is, pups in poor condition had fewer hookworms than those in good condition. There was a decline in the number of hookworms in sea lion pups in 2000 compared to collections in 1996. Eggs of Uncinaria spp. were found in rectal feces (collected in late September and early October) of none of 35 (0%) live fur seal pups and 41 of 48 (85%) live sea lion pups. Packed cell volume values, determined for most of the same live pups, were essentially normal for C. ursinus but were much lower than normal for most Z. californianus. Hookworm larvae were not found in blubber of fur seal and sea lion pups or in rookery sand in July. Rookery sand, positive for live hookworm larvae when put in a refrigerator, was negative at removal 2.5 years later. The average number of eggs in utero of female hookworms was 285 for three specimens from a fur seal pup and 281 from three specimens from a sea lion pup. One hookworm larva was recovered from milk stripped from the teats of a stranded Z. californianus female at The Marine Mammal Center, Sausalito, CA.

  19. Investigations of peritoneal and intestinal infections of adult hookworms (Uncinaria spp.) in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, California (2003).

    PubMed

    Lyons, Eugene T; Delong, R L; Nadler, S A; Laake, J L; Orr, A J; Delong, B L; Pagan, C

    2011-09-01

    The peritoneal cavity (PNC) and intestine of northern fur seal (Callorhinus ursinus) pups and California sea lion (Zalophus californianus) pups that died in late July and early August, 2003, on San Miguel Island, California, were examined for hookworms. Prevalence and morphometric studies were done with the hookworms in addition to molecular characterization. Based on this and previous molecular studies, hookworms from fur seals are designated as Uncinaria lucasi and the species from sea lions as Uncinaria species A. Adult hookworms were found in the PNC of 35 of 57 (61.4%) fur seal pups and of 13 of 104 (12.5%) sea lion pups. The number of hookworms located in the PNC ranged from 1 to 33 (median = 3) for the infected fur seal pups and 1 to 16 (median = 2) for the infected sea lion pups. In addition to the PNC, intestines of 43 fur seal and 32 sea lion pups were examined. All of these pups were positive for adult hookworms. The worms were counted from all but one of the sea lion pups. Numbers of these parasites in the intestine varied from 3 to 2,344 (median = 931) for the fur seal pups and 39 to 2,766 (median = 643) for the sea lion pups. Sea lion pups with peritoneal infections had higher intensity infections in the intestines than did pups without peritoneal infections, lending some support for the hypothesis that peritoneal infections result from high-intensity infections of adult worms. There was no difference in intestinal infection intensities between fur seal pups with and without peritoneal infections. Female adult hookworms in the intestines of both host species were significantly larger than males, and sea lion hookworms were larger than those in fur seals. Worms in the intestine also were larger than worms found in the PNC. Gene sequencing and (RFLP) analysis of (PCR) amplified (ITS) ribosomal DNA were used to diagnose the species of 172 hookworms recovered from the PNC and intestine of 18 C. ursinus and seven Z. californianus hosts

  20. Accelerometers Identify New Behaviors and Show Little Difference in the Activity Budgets of Lactating Northern Fur Seals (Callorhinus ursinus) between Breeding Islands and Foraging Habitats in the Eastern Bering Sea

    PubMed Central

    Battaile, Brian C.; Sakamoto, Kentaro Q.; Nordstrom, Chad A.; Rosen, David A. S.; Trites, Andrew W.

    2015-01-01

    We tagged 82 lactating northern fur seals (Callorhinus ursinus) with tri-axial accelerometers and magnetometers on two eastern Bering Sea islands (Bogoslof and St. Paul) with contrasting population trajectories. Using depth data, accelerometer data and spectral analysis we classified time spent diving (30%), resting (~7%), shaking and grooming their pelage (9%), swimming in the prone position (~10%) and two types of previously undocumented rolling behavior (29%), with the remaining time (~15%) unspecified. The reason for the extensive rolling behavior is not known. We ground-truthed the accelerometry signals for shaking and grooming and rolling behaviors—and identified the acceleration signal for porpoising—by filming tagged northern fur seals in captivity. Speeds from GPS interpolated data indicated that animals traveled fastest while in the prone position, suggesting that this behavior is indicative of destination-based swimming. Very little difference was found in the percentages of time spent in the categorical behaviors with respect to breeding islands (Bogoslof or St. Paul Island), forager type (cathemeral or nocturnal), and the region where the animals foraged (primarily on-shelf <200m, or off-shelf > 200m). The lack of significant differences between islands, regions and forager type may indicate that behaviors summarized over a trip are somewhat hardwired even though foraging trip length and when and where animals dive are known to vary with island, forager type and region. PMID:25807552

  1. Accelerometers identify new behaviors and show little difference in the activity budgets of lactating northern fur seals (Callorhinus ursinus) between breeding islands and foraging habitats in the eastern Bering Sea.

    PubMed

    Battaile, Brian C; Sakamoto, Kentaro Q; Nordstrom, Chad A; Rosen, David A S; Trites, Andrew W

    2015-01-01

    We tagged 82 lactating northern fur seals (Callorhinus ursinus) with tri-axial accelerometers and magnetometers on two eastern Bering Sea islands (Bogoslof and St. Paul) with contrasting population trajectories. Using depth data, accelerometer data and spectral analysis we classified time spent diving (30%), resting (~7%), shaking and grooming their pelage (9%), swimming in the prone position (~10%) and two types of previously undocumented rolling behavior (29%), with the remaining time (~15%) unspecified. The reason for the extensive rolling behavior is not known. We ground-truthed the accelerometry signals for shaking and grooming and rolling behaviors--and identified the acceleration signal for porpoising--by filming tagged northern fur seals in captivity. Speeds from GPS interpolated data indicated that animals traveled fastest while in the prone position, suggesting that this behavior is indicative of destination-based swimming. Very little difference was found in the percentages of time spent in the categorical behaviors with respect to breeding islands (Bogoslof or St. Paul Island), forager type (cathemeral or nocturnal), and the region where the animals foraged (primarily on-shelf <200 m, or off-shelf > 200 m). The lack of significant differences between islands, regions and forager type may indicate that behaviors summarized over a trip are somewhat hardwired even though foraging trip length and when and where animals dive are known to vary with island, forager type and region.

  2. Generic names of northern and southern fur seals (Mammalia: Otariidae)

    USGS Publications Warehouse

    Gardner, A.L.; Robbins, C.B.

    1998-01-01

    We have resolved a nomenclatural problem discovered during research on the northern fur seal that concerns the correct generic name for this taxon and for fur seals of the Southern Hemisphere. The unfortunate practice by some 19th century authors to use names in their Latinized form, but to date them from their first appearance as French common names led to the use of Arctocephalus for southern fur seals when the name correctly applies to the northern fur seal, known today as Callorhinus ursinus. However, Arctocephalus and Callorhinus are antedated by Otoes G. Fischer, 1817, which is the earliest available generic for the fur seal of the northern Pacific. The earliest available generic name for southern fur seals is Halarctus Gill, 1866. To avoid the confusion that would result from replacing the currently used generic names with those required by strict adherence to the Principle of Priority, we have petitioned the International Commission on Zoological nomenclature to preserve Arctocephalus and Callorhinus for the southern and northern fur seals, respectively.

  3. THE SHIFTING BASELINE OF NORTHERN FUR SEAL ECOLOGY IN THE NORTHEAST PACIFIC OCEAN

    EPA Science Inventory

    Historical data provide a baseline against which to judge the significance of recent ecological shifts and guide conservation strategies, especially for species decimated by pre-20th century harvesting. Northern fur seals (NFS; Callorhinus ursinus) are a common pinniped species i...

  4. TISSUE DISTRIBUTION OF PCBS AND ORGANOCHLORINE PESTICIDES IN ALASKAN NORTHERN FUR SEALS: COMPARISON OF VARIOUS CONGENER CLASSIFICATION SCHEMES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) are believed to adversely affect reproduction and cause health problems in Pinnipeds 1-4. In this study, 145 PCB congeners and OCPs were analyzed in 10 juvenile male northern fur seals, Callorhinus ursinus, collected from Alaskan...

  5. The oldest known fur seal

    PubMed Central

    Boessenecker, Robert W.; Churchill, Morgan

    2015-01-01

    The poorly known fossil record of fur seals and sea lions (Otariidae) does not reflect their current diversity and widespread abundance. This limited fossil record contrasts with the more complete fossil records of other pinnipeds such as walruses (Odobenidae). The oldest known otariids appear 5–6 Ma after the earliest odobenids, and the remarkably derived craniodental morphology of otariids offers few clues to their early evolutionary history and phylogenetic affinities among pinnipeds. We report a new otariid, Eotaria crypta, from the lower middle Miocene ‘Topanga’ Formation (15–17.1 Ma) of southern California, represented by a partial mandible with well-preserved dentition. Eotaria crypta is geochronologically intermediate between ‘enaliarctine’ stem pinnipedimorphs (16.6–27 Ma) and previously described otariid fossils (7.3–12.5 Ma), as well as morphologically intermediate by retaining an M2 and a reduced M1 metaconid cusp and lacking P2–4 metaconid cusps. Eotaria crypta eliminates the otariid ghost lineage and confirms that otariids evolved from an ‘enaliarctine’-like ancestor. PMID:25672999

  6. 50 CFR 216.71 - Allowable take of fur seals.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 10 2014-10-01 2014-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b)...

  7. 50 CFR 216.81 - Visits to fur seal rookeries.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Visits to fur seal rookeries. 216.81... MAMMALS Pribilof Islands Administration § 216.81 Visits to fur seal rookeries. From June 1 to October 15... any fur seal rookery or hauling grounds nor pass beyond any posted sign forbidding passage....

  8. 50 CFR 216.81 - Visits to fur seal rookeries.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 10 2014-10-01 2014-10-01 false Visits to fur seal rookeries. 216.81... MAMMALS Pribilof Islands Administration § 216.81 Visits to fur seal rookeries. From June 1 to October 15... any fur seal rookery or hauling grounds nor pass beyond any posted sign forbidding passage....

  9. 50 CFR 216.73 - Disposition of fur seal parts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 10 2014-10-01 2014-10-01 false Disposition of fur seal parts. 216.73... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.73 Disposition of fur seal parts. Except... part of a fur seal taken for subsistence uses may be sold or otherwise transferred to any person...

  10. 50 CFR 216.71 - Allowable take of fur seals.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b)...

  11. 50 CFR 216.73 - Disposition of fur seal parts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Disposition of fur seal parts. 216.73... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.73 Disposition of fur seal parts. Except... part of a fur seal taken for subsistence uses may be sold or otherwise transferred to any person...

  12. 50 CFR 216.71 - Allowable take of fur seals.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b)...

  13. 50 CFR 216.73 - Disposition of fur seal parts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Disposition of fur seal parts. 216.73... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.73 Disposition of fur seal parts. Except... part of a fur seal taken for subsistence uses may be sold or otherwise transferred to any person...

  14. 50 CFR 216.71 - Allowable take of fur seals.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b)...

  15. 50 CFR 216.81 - Visits to fur seal rookeries.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Visits to fur seal rookeries. 216.81... MAMMALS Pribilof Islands Administration § 216.81 Visits to fur seal rookeries. From June 1 to October 15... any fur seal rookery or hauling grounds nor pass beyond any posted sign forbidding passage....

  16. 50 CFR 216.73 - Disposition of fur seal parts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false Disposition of fur seal parts. 216.73... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.73 Disposition of fur seal parts. Except... part of a fur seal taken for subsistence uses may be sold or otherwise transferred to any person...

  17. 50 CFR 216.81 - Visits to fur seal rookeries.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false Visits to fur seal rookeries. 216.81... MAMMALS Pribilof Islands Administration § 216.81 Visits to fur seal rookeries. From June 1 to October 15... any fur seal rookery or hauling grounds nor pass beyond any posted sign forbidding passage....

  18. 50 CFR 216.71 - Allowable take of fur seals.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b)...

  19. 50 CFR 216.73 - Disposition of fur seal parts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false Disposition of fur seal parts. 216.73... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.73 Disposition of fur seal parts. Except... part of a fur seal taken for subsistence uses may be sold or otherwise transferred to any person...

  20. 50 CFR 216.81 - Visits to fur seal rookeries.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false Visits to fur seal rookeries. 216.81... MAMMALS Pribilof Islands Administration § 216.81 Visits to fur seal rookeries. From June 1 to October 15... any fur seal rookery or hauling grounds nor pass beyond any posted sign forbidding passage....

  1. Extreme weather events influence dispersal of naive northern fur seals.

    PubMed

    Lea, Mary-Anne; Johnson, Devin; Ream, Rolf; Sterling, Jeremy; Melin, Sharon; Gelatt, Tom

    2009-04-23

    Since 1975, northern fur seal (Callorhinus ursinus) numbers at the Pribilof Islands (PI) in the Bering Sea have declined rapidly for unknown reasons. Migratory dispersal and habitat choice may affect first-year survivorship, thereby contributing to this decline. We compared migratory behaviour of 166 naive pups during 2 years from islands with disparate population trends (increasing: Bogoslof and San Miguel Islands; declining: PI), hypothesizing that climatic conditions at weaning may differentially affect dispersal and survival. Atmospheric conditions (Bering Sea) in autumn 2005-2006 were anomalously cold, while 2006-2007 was considerably warmer and less stormy. In 2005, pups departed earlier at all sites, and the majority of PI pups (68-85%) departed within 1 day of Arctic storms and dispersed quickly, travelling southwards through the Aleutian Islands. Tailwinds enabled faster rates of travel than headwinds, a trend not previously shown for marine mammals. Weather effects were less pronounced at Bogoslof Island (approx. 400 km further south), and, at San Miguel Island, (California) departures were more gradual, and only influenced by wind and air pressure in 2005. We suggest that increasingly variable climatic conditions at weaning, particularly timing, frequency and intensity of autumnal storms in the Bering Sea, may alter timing, direction of dispersal and potentially survival of pups.

  2. Feeding kinematics and performance of basal otariid pinnipeds, Steller sea lions and northern fur seals: implications for the evolution of mammalian feeding.

    PubMed

    Marshall, Christopher D; Rosen, David A S; Trites, Andrew W

    2015-10-01

    Feeding performance studies can address questions relevant to feeding ecology and evolution. Our current understanding of feeding mechanisms for aquatic mammals is poor. Therefore, we characterized the feeding kinematics and performance of five Steller sea lions (Eumetopias jubatus) and six northern fur seals (Callorhinus ursinus). We tested the hypotheses that both species use suction as their primary feeding mode, and that rapid jaw opening was related to suction generation. Steller sea lions used suction as their primary feeding mode, but also used a biting feeding mode. In contrast, northern fur seals only used a biting feeding mode. Kinematic profiles of Steller sea lions were all indicative of suction feeding (i.e. a small gape, small gape angle, large depression of the hyolingual apparatus and lip pursing). However, jaw opening as measured by gape angle opening velocity (GAOV) was relatively slow in Steller sea lions. In contrast to Steller sea lions, the GAOV of northern fur seals was extremely fast, but their kinematic profiles indicated a biting feeding mode (i.e. northern fur seals exhibited a greater gape, a greater gape angle and minimal depression of the hyolingual apparatus compared with Steller sea lions). Steller sea lions produced both subambient and suprambient pressures at 45 kPa. In contrast, northern fur seals produced no detectable pressure measurements. Steller sea lions have a broader feeding repertoire than northern fur seals, which likely enables them to feed on a greater variety of prey, in more diverse habitats. Based on the basal phylogenetic position of northern fur seals, craniodental morphological data of the Callorhinus lineage, and the performance data provided in this study, we suggest that northern fur seals may be exhibiting their ancestral feeding mode.

  3. Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery.

    PubMed

    Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; Williams, Michael; Gelatt, Thomas; Bell, Justin; Johnson, Thomas E

    2016-02-01

    In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimum detectable activity concentrations of (137)Cs and (134)Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq (134)Cs kg(-1) f.w. (95% CI: 35.9-38.5) and 141.2 mBq (137)Cs kg(-1) f.w. (95% CI: 135.5-146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25-0.28). The Fukushima nuclear accident released (134)Cs and (137)Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both (134)Cs and (137)Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace (134)Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species.

  4. Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

    DOE PAGES

    Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; ...

    2015-11-28

    In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimummore » detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg-1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg-1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species.« less

  5. Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

    SciTech Connect

    Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; Williams, Michael; Gelatt, Thomas; Bell, Justin; Johnson, Thomas E.

    2015-11-28

    In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimum detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg-1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg-1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation

  6. Monoamine Release during Unihemispheric Sleep and Unihemispheric Waking in the Fur Seal

    PubMed Central

    Lyamin, Oleg I.; Lapierre, Jennifer L.; Kosenko, Peter O.; Kodama, Tohru; Bhagwandin, Adhil; Korneva, Svetlana M.; Peever, John H.; Mukhametov, Lev M.; Siegel, Jerome M.

    2016-01-01

    Study Objectives: Our understanding of the role of neurotransmitters in the control of the electroencephalogram (EEG) has been entirely based on studies of animals with bilateral sleep. The study of animals with unihemispheric sleep presents the opportunity of separating the neurochemical substrates of waking and sleep EEG from the systemic, bilateral correlates of sleep and waking states. Methods: The release of histamine (HI), norepinephrine (NE), and serotonin (5HT) in cortical and subcortical areas (hypothalamus, thalamus and caudate nucleus) was measured in unrestrained northern fur seals (Callorhinus ursinus) using in vivo microdialysis, in combination with, polygraphic recording of EEG, electrooculogram, and neck electromyogram. Results: The pattern of cortical and subcortical HI, NE, and 5HT release in fur seals is similar during bilaterally symmetrical states: highest in active waking, reduced in quiet waking and bilateral slow wave sleep, and lowest in rapid eye movement (REM) sleep. Cortical and subcortical HI, NE, and 5HT release in seals is highly elevated during certain waking stimuli and behaviors, such as being sprayed with water and feeding. However, in contrast to acetylcholine (ACh), which we have previously studied, the release of HI, NE, and 5HT during unihemispheric sleep is not lateralized in the fur seal. Conclusions: Among the studied neurotransmitters most strongly implicated in waking control, only ACh release is asymmetric in unihemispheric sleep and waking, being greatly increased on the activated side of the brain. Commentary: A commentary on this article appears in this issue on page 491. Citation: Lyamin OI, Lapierre JL, Kosenko PO, Kodama T, Bhagwandin A, Korneva SM, Peever JH, Mukhametov LM, Siegel JM. Monoamine release during unihemispheric sleep and unihemispheric waking in the fur seal. SLEEP 2016;39(3):625–636. PMID:26715233

  7. Foraging habitats of lactating northern fur seals are structured by thermocline depths and submesoscale fronts in the eastern Bering Sea

    NASA Astrophysics Data System (ADS)

    Nordstrom, Chad A.; Battaile, Brian C.; Cotté, Cédric; Trites, Andrew W.

    2013-04-01

    The relationships between fine-scale oceanographic features, prey aggregations, and the foraging behavior of top predators are poorly understood. We investigated whether foraging patterns of lactating northern fur seals (Callorhinus ursinus) from two breeding colonies located in different oceanographic domains of the eastern Bering Sea (St. Paul Island—shelf; Bogoslof Island—oceanic) were a function of submesoscale oceanographic features. We tested this by tracking 87 lactating fur seals instrumented with bio-logging tags (44 St. Paul Island, 43 Bogoslof Island) during July-September, 2009. We identified probable foraging hotspots using first-passage time analysis and statistically linked individual areas of high-use to fine-scale oceanographic features using mixed-effects Cox-proportional hazard models. We found no overlap in foraging areas used by fur seals from the two islands, but a difference in the duration of their foraging trips—trips from St. Paul Island were twice as long (7.9 d average) and covered 3-times the distance (600 km average) compared to trips from Bogoslof Island. St. Paul fur seals also foraged at twice the scale (mean radius=12 km) of Bogoslof fur seals (6 km), which suggests that prey were more diffuse near St. Paul Island than prey near Bogoslof Island. Comparing first passage times with oceanographic covariates revealed that foraging hotspots were linked to thermocline depth and occurred near submesoscale surface fronts (eddies and filaments). St. Paul fur seals that mixed epipelagic (night) and benthic (day) dives primarily foraged on-shelf in areas with deeper thermoclines that may have concentrated prey closer to the ocean floor, while strictly epipelagic (night) foragers tended to use waters with shallower thermoclines that may have aggregated prey closer to the surface. Fur seals from Bogoslof Island foraged almost exclusively over the Bering Sea basin and appeared to hunt intensively along submesoscale fronts that may have

  8. 50 CFR 223.201 - Guadalupe fur seal.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 10 2014-10-01 2014-10-01 false Guadalupe fur seal. 223.201 Section 223... Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The prohibitions of section 9 of the Act (16 U.S.C. 1538) relating to endangered species apply to the Guadalupe...

  9. Serum chemistry and antibodies against pathogens in antarctic fur seals, Weddell seals, crabeater seals, and Ross seals.

    PubMed

    Tryland, Morten; Nymo, Ingebjørg H; Nielsen, Ole; Nordøy, Erling S; Kovacs, Kit M; Krafft, Bjørn A; Thoresen, Stein I; Åsbakk, Kjetil; Osterrieder, Klaus; Roth, Swaantje J; Lydersen, Christian; Godfroid, Jacques; Blix, Arnoldus S

    2012-07-01

    Information on health parameters, such as antibody prevalences and serum chemistry that can reveal exposure to pathogens, disease, and abnormal physiologic conditions, is scarce for Antarctic seal species. Serum samples from Antarctic fur seals (Arctocephalus gazella, n=88) from Bouvetøya (2000-2001 and 2001-2002), and from Weddell seals (Leptonychotes weddellii, n=20), Ross seals (Ommatophoca rossii, n=20), and crabeater seals (Lobodon carcinophagus, n=9) from the pack-ice off Queen Maud Land, Antarctica (2001) were analyzed for enzyme activity, and concentrations of protein, metabolites, minerals, and cortisol. Adult Antarctic fur seal males had elevated levels of total protein (range 64-99 g/l) compared to adult females and pups (range 52-79 g/l). Antarctic fur seals had higher enzyme activities of creatine kinase, lactate dehydrogenase, and amylase, compared to Weddell, Ross, and crabeater seals. Antibodies against Brucella spp. were detected in Weddell seals (37%), Ross seals (5%), and crabeater seals (11%), but not in Antarctic fur seals. Antibodies against phocine herpesvirus 1 were detected in all species examined (Antarctic fur seals, 58%; Weddell seals, 100%; Ross seals, 15%; and crabeater seals, 44%). No antibodies against Trichinella spp., Toxoplasma, or phocine distemper virus (PDV) were detected (Antarctic fur seals were not tested for PDV antibodies). Antarctic seals are challenged by reduced sea ice and increasing temperatures due to climate change, and increased anthropogenic activity can introduce new pathogens to these vulnerable ecosystems and represent a threat for these animals. Our data provide a baseline for future monitoring of health parameters of these Antarctic seal species, for tracking the impact of environmental, climatic, and anthropogenic changes in Antarctica over time.

  10. 50 CFR 223.201 - Guadalupe fur seal.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....201 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions....

  11. Brightness discrimination in the South African fur seal (Arctocephalus pusillus).

    PubMed

    Scholtyssek, C; Dehnhardt, G

    2013-05-24

    Underwater, the contrast between object and background is much larger reduced with increasing distance between object and observer than in air. For marine predators, such as pinnipeds, it would therefore be advantageous to possess a high sensitivity for brightness differences, since this would increase the distance at which prey can be detected visually. Few studies have examined the brightness discrimination thresholds of pinnipeds. Two studies with phocid seals have confirmed low brightness discrimination thresholds in pinnipeds whereas the threshold obtained for the South African fur seal seems to be twice as high as that of the phocids. However, the experiments with the South African fur seal have been conducted under inadequate conditions which likely resulted in an underestimation of the brightness discrimination ability of this species. The study at hand reinvestigated the brightness discrimination threshold of the South African fur seal under well controlled conditions. In a two alternative forced choice task, one fur seal was trained to indicate the position of the brighter of two gray discs presented on a black background on a monitor. The thresholds were determined for 11 standard intensities each tested against 8 lower comparison intensities. It was found that the fur seal was able to perceive brightness differences of 8-10%, which is better than the phocid species tested so far. For low standard intensities, however, the threshold increased which could to be due to a relative slow dark adaptation rate of the fur seal. The results are discussed in terms of the relevance of visual information for pinnipeds during foraging dives and are directly compared to the results obtained for the harbor seal which has been tested under the same conditions as the fur seal in a previous study.

  12. 77 FR 6682 - Marine Mammals; Subsistence Taking of Northern Fur Seals; Harvest Estimates

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-09

    ...), National Oceanic and Atmospheric Administration (NOAA), Commerce. ACTION: Final estimates of annual fur... ] northern fur seals, NMFS is publishing the annual fur seal subsistence harvests on St. George and St. Paul Islands (the Pribilof Islands) for 2008 to 2010, and the annual estimates for the fur seal...

  13. 19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or...

  14. 19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or...

  15. 19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or...

  16. 19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or...

  17. 19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or...

  18. 77 FR 41168 - Marine Mammals; Subsistence Taking of Northern Fur Seals; St. Paul Island

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-12

    ... Northern Fur Seals; St. Paul Island AGENCY: National Marine Fisheries Service (NMFS), National Oceanic and...) petitioned NMFS to revise regulations governing the subsistence taking of northern fur seals on St. Paul..., Alaska to: Take male young of the year (less than 1 year old) fur seals; take a total of up to 3,000...

  19. 50 CFR 223.201 - Guadalupe fur seal.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The prohibitions of section 9 of the Act (16 U.S.C. 1538) relating to endangered species apply to the Guadalupe...

  20. 50 CFR 223.201 - Guadalupe fur seal.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The prohibitions of section 9 of the Act (16 U.S.C. 1538) relating to endangered species apply to the Guadalupe...

  1. Regional differences in cortical electroencephalogram (EEG) slow wave activity and interhemispheric EEG asymmetry in the fur seal.

    PubMed

    Lyamin, Oleg I; Pavlova, Ivetta F; Kosenko, Peter O; Mukhametov, Lev M; Siegel, Jerome M

    2012-12-01

    Slow wave sleep (SWS) in the northern fur seal (Callorhinus ursinus) is characterized by a highly expressed interhemispheric electroencephalogram (EEG) asymmetry, called 'unihemispheric' or 'asymmetrical' SWS. The aim of this study was to examine the regional differences in slow wave activity (SWA; power in the range of 1.2-4.0 Hz) within one hemisphere and differences in the degree of interhemispheric EEG asymmetry within this species. Three seals were implanted with 10 EEG electrodes, positioned bilaterally (five in each hemisphere) over the frontal, occipital and parietal cortex. The expression of interhemispheric SWA asymmetry between symmetrical monopolar recordings was estimated based on the asymmetry index [AI = (L-R)/(L+R), where L and R are the power in the left and right hemispheres, respectively]. Our findings indicate an anterior-posterior gradient in SWA during asymmetrical SWS in fur seals, which is opposite to that described for other mammals, including humans, with a larger SWA recorded in the parietal and occipital cortex. Interhemispheric EEG asymmetry in fur seals was recorded across the entire dorsal cerebral cortex, including sensory (visual and somatosensory), motor and associative (parietal or suprasylvian) cortical areas. The expression of asymmetry was greatest in occipital-lateral and parietal derivations and smallest in frontal-medial derivations. Regardless of regional differences in SWA, the majority (90%) of SWS episodes with interhemispheric EEG asymmetry meet the criteria for 'unihemispheric SWS' (one hemisphere is asleep while the other is awake). The remaining episodes can be described as episodes of bilateral SWS with a local activation in one cerebral hemisphere.

  2. Morphometric and molecular characterization of the species of Uncinaria Frölich, 1789 (Nematoda) parasitic in the Australian fur seal Arctocephalus pusillus doriferus (Schreber), with notes on hookworms in three other pinniped hosts.

    PubMed

    Ramos, Paul; Lynch, Michael; Hu, Min; Arnould, John P Y; Norman, Richard; Beveridge, Ian

    2013-05-01

    This study presents morphological and molecular data on hookworms from the Australian fur seal Arctocephalus pusillus doriferus (Schreber) currently identified in Australian waters as Uncinaria hamiltoni Baylis, 1933. Additional specimens from the Australian sea lion Neophoca cinerea (Péron) and the New Zealand fur seal Arctocephalus forsteri (Lesson) from Australia, and the Southern elephant seal Mirounga leonina (Linnaeus) from Antarctica, were included. Using the internal transcribed spacer (ITS), hookworms from A. p. doriferus, N. cinerea and A. forsteri were found to be genetically similar but distinct from Uncinaria spp. found in M. leonina from Antarctica, as well as from Zalophus californianus (Lesson) and Callorhinus ursinus (Linnaeus) from California. Few morphological differences were detected between these taxa.

  3. Isolation and characterization of Campylobacter spp. from Antarctic fur seals (Arctocephalus gazella) at Deception Island, Antarctica.

    PubMed

    García-Peña, F J; Pérez-Boto, D; Jiménez, C; San Miguel, E; Echeita, A; Rengifo-Herrera, C; García-Párraga, D; Ortega-Mora, L M; Pedraza-Díaz, S

    2010-09-01

    The presence of Campylobacter spp. was investigated in 41 Antarctic fur seals (Arctocephalus gazella) and 9 Weddell seals (Leptonychotes weddellii) at Deception Island, Antarctica. Infections were encountered in six Antarctic fur seals. The isolates, the first reported from marine mammals in the Antarctic region, were identified as Campylobacter insulaenigrae and Campylobacter lari.

  4. [Frequency discrimination by the bottle-nosed dolphin and the Northern fur seal depending on sound parameters and sound conduction pathways].

    PubMed

    Babushkina, E S; Poliakov, M A

    2003-01-01

    Underwater differential frequency hearing thresholds in the Black Sea bottle-nosed dolphin (Tursiops truncatus p.) and the northern fur seal (Callorhinus ursinus) were measured depending on signal frequency and sound conduction pathways. The measurements were performed by the method of instrumental conditioned reflexes with food reinforcement under conditions of full and partial (with heads out of water at sound conduction through body tissues) submergence of animals into water. It was shown that in a frequency range of 5-100 kHz, underwater differential frequency hearing thresholds of the bottle-nosed dolphin changed from 0.46-0.60% to 0.21-0.34% and depended little on sound conduction pathways. The minimum underwater differential frequency hearing thresholds of the northern fur seal corresponded to the frequencies of maximum hearing sensitivity, changed from 1.7% to 1-2.3% in a frequency range of 1-20 kHz, sharply increased at the edges of the frequency hearing perception range, and depended little (in a range of 5-40 kHz) on sound conduction pathways. Thus, underwater sounds propagating through the body tissues of dolphin and fur seal reach the inner ear.

  5. Molecular and morphometric evidence for separate species of Uncinaria (Nematoda: Ancylostomatidae) in California sea lions and northern fur seals: hypothesis testing supplants verification.

    PubMed

    Nadler, S A; Adams, B J; Lyons, E T; DeLong, R L; Melin, S R

    2000-10-01

    California sea lions (Zalophus californianus) and northern fur seals (Callorhinus ursinus) are each believed to host distinct hookworm species (Uncinaria spp.). However, a recent morphometric analysis suggested that a single species parasitizes multiple pinniped hosts, and that the observed differences are host-induced. To explore the systematics of these hookworms and test these competing hypotheses, we obtained nucleotide sequences of nuclear ribosomal DNA (D2/D3 28S, D18/D19 28S, and internal transcribed spacer [ITS] regions) from 20 individual hookworms parasitizing California sea lion and northern fur seal pups where their breeding grounds are sympatric. Five individuals from an allopatric population of California sea lions were also sampled for ITS-1 and D18/D19 28S sequences. The 28S D2/D3 sequences showed no diagnostic differences among hookworms sampled from individual sea lions and fur seals, whereas the 28S D18/D19 sequences had one derived (apomorphic) character demarcating hookworms from northern fur seals. ITS sequences were variable for 7 characters, with 4 derived (apomorphic) states in ITS-1 demarcating hookworms from California sea lions. Multivariate analysis of morphometric data also revealed significant differences between nematodes representing these 2 host-associated lineages. These results indicate that these hookworms represent 2 species that are not distributed indiscriminately between these host species, but instead exhibit host fidelity, evolving independently with each respective host species. This evolutionary approach to analyzing sequence data for species delimitation is contrasted with similarity-based methods that have been applied to numerous diagnostic studies of nematode parasites.

  6. Preliminary investigation of a possible lung worm (Parafilaroides decorus), fish (Girella nigricans), and marine mammal (Callorhinus ursinus) cycle for San Miguel sea lion virus type 5.

    PubMed

    Smith, A W; Skilling, D E; Brown, R J

    1980-11-01

    Colostrum-deprived neonatal Northern fur seal pups (Callorhinus ursinus) were exposed to San Miguel sea lion virus type 5 (SMSV-5) by feeding them fish (Girella nigricans) infected with virus or fish infected with both the sea lion lung worm larvae (Parafilaroides decorus) and virus. Virus infection was demonstrated in 8 of 9 pups, and 1 of these developed a vesicular lesion on the flipper. In this sequence, P decorus larvae exposed to SMSV-5 were fed to G nigricans held at 15 C in a salt water aquarium; 32 days later, these fish were killed, then fed to the fur seal pups. The vesicle developed 22 days subsequent to this and SMSV-5 was reisolated from the lesion. The SMSV-5 was shown to persist for at least 23 days in infected neonatal fur seals. Attempts to establish P decorus infection in Northern fur seal pups were apparently unsuccessful.

  7. Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals

    PubMed Central

    Kluge, Mariana; Campos, Fabrício Souza; Tavares, Maurício; de Amorim, Derek Blaese; Valdez, Fernanda Pedone; Giongo, Adriana; Roehe, Paulo Michel; Franco, Ana Claudia

    2016-01-01

    The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals. PMID:26986573

  8. Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals.

    PubMed

    Kluge, Mariana; Campos, Fabrício Souza; Tavares, Maurício; de Amorim, Derek Blaese; Valdez, Fernanda Pedone; Giongo, Adriana; Roehe, Paulo Michel; Franco, Ana Claudia

    2016-01-01

    The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals.

  9. Encephalitozoonosis in 2 South American Fur Seal (Arctocephalus australis) Pups

    PubMed Central

    Seguel, M.; Howerth, E. W.; Ritter, J.; Paredes, E.; Colegrove, K.; Gottdenker, N.

    2015-01-01

    Cerebral and disseminated encephalitozoonosis was diagnosed by histopathology, electron microscopy, and immunohistochemistry in 2 free-ranging South American fur seal pups found dead at Guafo Island (43°33′S 74°49′W) in southern Chile. In the brain, lesions were characterized by random foci of necrosis with large numbers of macrophages containing numerous microsporidial organisms within parasitophorous vacuoles. In addition, occasional histiocytes loaded with numerous mature and immature microsporidia spores consistent with Encephalitozoon sp were observed in pulmonary alveolar septa, splenic red pulp, glomerular capillaries, and proximal renal tubules by Gram and immunohistochemical stains. To our knowledge, microsporidial infection in a marine mammal species has not been previously reported. PMID:25248519

  10. Utilisation of Intensive Foraging Zones by Female Australian Fur Seals

    PubMed Central

    Hoskins, Andrew J.; Costa, Daniel P.; Arnould, John P. Y.

    2015-01-01

    Within a heterogeneous environment, animals must efficiently locate and utilise foraging patches. One way animals can achieve this is by increasing residency times in areas where foraging success is highest (area-restricted search). For air-breathing diving predators, increased patch residency times can be achieved by altering both surface movements and diving patterns. The current study aimed to spatially identify the areas where female Australian fur seals allocated the most foraging effort, while simultaneously determining the behavioural changes that occur when they increase their foraging intensity. To achieve this, foraging behaviour was successfully recorded with a FastLoc GPS logger and dive behaviour recorder from 29 individual females provisioning pups. Females travelled an average of 118 ± 50 km from their colony during foraging trips that lasted 7.3 ± 3.4 days. Comparison of two methods for calculating foraging intensity (first-passage time and first-passage time modified to include diving behaviour) determined that, due to extended surface intervals where individuals did not travel, inclusion of diving behaviour into foraging analyses was important for this species. Foraging intensity ‘hot spots’ were found to exist in a mosaic of patches within the Bass Basin, primarily to the south-west of the colony. However, the composition of benthic habitat being targeted remains unclear. When increasing their foraging intensity, individuals tended to perform dives around 148 s or greater, with descent/ascent rates of approximately 1.9 m•s-1 or greater and reduced postdive durations. This suggests individuals were maximising their time within the benthic foraging zone. Furthermore, individuals increased tortuosity and decreased travel speeds while at the surface to maximise their time within a foraging location. These results suggest Australian fur seals will modify both surface movements and diving behaviour to maximise their time within a foraging patch

  11. Utilisation of intensive foraging zones by female Australian fur seals.

    PubMed

    Hoskins, Andrew J; Costa, Daniel P; Arnould, John P Y

    2015-01-01

    Within a heterogeneous environment, animals must efficiently locate and utilise foraging patches. One way animals can achieve this is by increasing residency times in areas where foraging success is highest (area-restricted search). For air-breathing diving predators, increased patch residency times can be achieved by altering both surface movements and diving patterns. The current study aimed to spatially identify the areas where female Australian fur seals allocated the most foraging effort, while simultaneously determining the behavioural changes that occur when they increase their foraging intensity. To achieve this, foraging behaviour was successfully recorded with a FastLoc GPS logger and dive behaviour recorder from 29 individual females provisioning pups. Females travelled an average of 118 ± 50 km from their colony during foraging trips that lasted 7.3 ± 3.4 days. Comparison of two methods for calculating foraging intensity (first-passage time and first-passage time modified to include diving behaviour) determined that, due to extended surface intervals where individuals did not travel, inclusion of diving behaviour into foraging analyses was important for this species. Foraging intensity 'hot spots' were found to exist in a mosaic of patches within the Bass Basin, primarily to the south-west of the colony. However, the composition of benthic habitat being targeted remains unclear. When increasing their foraging intensity, individuals tended to perform dives around 148 s or greater, with descent/ascent rates of approximately 1.9 m•s-1 or greater and reduced postdive durations. This suggests individuals were maximising their time within the benthic foraging zone. Furthermore, individuals increased tortuosity and decreased travel speeds while at the surface to maximise their time within a foraging location. These results suggest Australian fur seals will modify both surface movements and diving behaviour to maximise their time within a foraging patch.

  12. Bilateral ocular anomalies in a South African fur seal (Arctocephalus pusillus pusillus).

    PubMed

    Colitz, Carmen M H; Rudnick, Jens-Christian; Heegaard, Steffen

    2014-07-01

    A female South African fur seal (Arctocephalus pusillus pusillus) began having obvious clinical ophthalmologic problems by 8 weeks of age. The initial clinical sign was diffuse corneal edema, which progressed to bullae formation and ulcers; the underlying cause of corneal edema and bullous keratopathy was not identified antemortem.An ophthalmological evaluation was performed when the fur seal was approximately 6 months of age, but due to the diffuse corneal edema, intraocular structures could not be easily evaluated. An underlying infectious etiology was suspected; therefore,appropriate diagnostics were pursued, but did not identify a cause. Initial improvement was noted, but the fur seal then became blind and eventually became very painful.Due to decreased quality of life and aggressive behavior, the fur seal was euthanized.Histopathological diagnoses were persistent tunica vasculosa lentis and persistent hyperplastic primary vitreous with bilateral hypermature resorbed cataracts and retinal detachments with rosette formation.

  13. The behavioural response of Australian fur seals to motor boat noise.

    PubMed

    Tripovich, Joy S; Hall-Aspland, Sophie; Charrier, Isabelle; Arnould, John P Y

    2012-01-01

    Australian fur seals breed on thirteen islands located in the Bass Strait, Australia. Land access to these islands is restricted, minimising human presence but boat access is still permissible with limitations on approach distances. Thirty-two controlled noise exposure experiments were conducted on breeding Australian fur seals to determine their behavioural response to controlled in-air motor boat noise on Kanowna Island (39°10'S, 146°18'E). Our results show there were significant differences in the seals' behaviour at low (64-70 dB) versus high (75-85 dB) sound levels, with seals orientating themselves towards or physically moving away from the louder boat noise at three different sound levels. Furthermore, seals responded more aggressively with one another and were more alert when they heard louder boat noise. Australian fur seals demonstrated plasticity in their vocal responses to boat noise with calls being significantly different between the various sound intensities and barks tending to get faster as the boat noise got louder. These results suggest that Australian fur seals on Kanowna Island show behavioural disturbance to high level boat noise. Consequently, it is recommended that an appropriate level of received boat sound emissions at breeding fur seal colonies be below 74 dB and that these findings be taken into account when evaluating appropriate approach distances and speed limits for boats.

  14. The Behavioural Response of Australian Fur Seals to Motor Boat Noise

    PubMed Central

    Tripovich, Joy S.; Hall-Aspland, Sophie; Charrier, Isabelle; Arnould, John P. Y.

    2012-01-01

    Australian fur seals breed on thirteen islands located in the Bass Strait, Australia. Land access to these islands is restricted, minimising human presence but boat access is still permissible with limitations on approach distances. Thirty-two controlled noise exposure experiments were conducted on breeding Australian fur seals to determine their behavioural response to controlled in-air motor boat noise on Kanowna Island (39°10′S, 146°18′E). Our results show there were significant differences in the seals' behaviour at low (64–70 dB) versus high (75–85 dB) sound levels, with seals orientating themselves towards or physically moving away from the louder boat noise at three different sound levels. Furthermore, seals responded more aggressively with one another and were more alert when they heard louder boat noise. Australian fur seals demonstrated plasticity in their vocal responses to boat noise with calls being significantly different between the various sound intensities and barks tending to get faster as the boat noise got louder. These results suggest that Australian fur seals on Kanowna Island show behavioural disturbance to high level boat noise. Consequently, it is recommended that an appropriate level of received boat sound emissions at breeding fur seal colonies be below 74 dB and that these findings be taken into account when evaluating appropriate approach distances and speed limits for boats. PMID:22623998

  15. A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation.

    PubMed

    Pharo, Elizabeth A; Cane, Kylie N; McCoey, Julia; Buckle, Ashley M; Oosthuizen, W H; Guinet, Christophe; Arnould, John P Y

    2016-03-01

    The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48 h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3 months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk.

  16. Hookworm Infection in South American Fur Seal ( Arctocephalus australis) Pups.

    PubMed

    Seguel, M; Muñoz, F; Navarrete, M J; Paredes, E; Howerth, E; Gottdenker, N

    2017-03-01

    Tissues of South American fur seal pups naturally infected with hookworms ( Uncinaria sp) were examined. Hookworm infection was found in nearly all pups examined (132/140, 94%), and hookworm enteritis with secondary bacteremia was considered the cause of death in 46 (35%) pups. Common findings in these pups included severe hemorrhagic enteritis and numerous (mean intensity = 761.8) hookworms in the jejunum. Hookworms were recovered from the abdominal cavity in 12 of 55 pups (22%) examined through peritoneal wash; these pups had an average of 1343.3 intestinal hookworms and marked fibrinohemorrhagic peritonitis. In all pups that died as a consequence of hookworm infection, the intestinal villi were short, blunt, and fused, and there were variable numbers of free and intrahistiocytic gram-negative bacteria in submucosal hookworm feeding tracks, mesenteric lymph nodes, spleen, blood vessels, and liver sinusoids. Pups that died of causes unrelated to the hookworm infection (trauma) had hookworm feeding tracks confined to the apical portions of the mucosa, and moderate to marked catarrhal eosinophilic enteritis. The number of hookworms was negatively correlated with intestinal villous length and number of leukocytes in the intestine. Pups with hookworm peritoneal penetration had nematodes with little or no blood in the hookworm intestine, suggesting that lack of food for the nematode could be associated with peritoneal penetration. Findings suggest that the initial burden of larval infection, the level of the host tissue response, or a combination determine the number of nematodes in the intestine, the severity of hookworm tissue damage, and pup mortality.

  17. Age-related differences revealed in Australian fur seal Arctocephalus pusillus doriferus gut microbiota.

    PubMed

    Smith, Stuart C; Chalker, Andrea; Dewar, Meagan L; Arnould, John P Y

    2013-11-01

    The gut microbiota of Australian fur seals (Arctocephalus pusillus doriferus) was examined at different age classes using fluorescent in situ hybridisation (FISH) and 16S rRNA gene pyrosequencing. The FISH results indicated that in the fur seal groups, the predominant phyla are Firmicutes (22.14-67.33%) followed by Bacteroidetes (3.11-15.45%) and then Actinobacteria (1.4-5.9%) consistent with other mammals. Phylum Proteobacteria had an initial abundance of 1.8% in the 2-month-old pups, but < 1% of bacterial numbers for the other fur seal age groups. Significant differences did occur in the abundance of Clostridia, Lactobacilli and Bifidobacteria between 2 months pups and 9 months pups and adult fur seals. Results from the 16S rRNA gene pyrosequencing supported the FISH data and identified significant differences in the composition of Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Verrucomicrobia and Fusobacteria at all ages. Class Clostridia in phylum Firmicutes dominates the microbiota of the 2 months and 9 months seal pups, whilst class Bacilli dominates the 6 months pups. In addition, a high level of dissimilarity was observed between all age classes. This study provides novel insight into the gut microbiota of Australian fur seals at different age classes.

  18. Treatment and prevention of infection following bites of the Antarctic fur seal (Arctocephalus gazella)

    PubMed Central

    Kouliev, Timur; Cui, Victoria

    2015-01-01

    In recent decades, an increasing number of people have traveled to sub-Antarctic and Antarctic regions each year for research, tourism, and resource exploitation. Hunting of Antarctic fur seals (Arctocephalus gazella) almost pushed the species to extinction in the early 1900s, but populations have since shown rapid and substantial recovery. The species’ range has re-expanded to include several islands south of the Antarctic Convergence, most notably South Georgia, and now overlaps with many popular Antarctic travel destinations. Both male and female fur seals can become extremely aggressive when provoked, and their bites, if not properly treated, pose a significant risk of infection by microorganisms not usually encountered in cases of animal bites. In this report, we present the case of a patient treated for a fur seal bite during an Antarctic expedition cruise, review the literature concerning seal bites, and suggest the use of antibiotic prophylaxis to prevent complications. PMID:27147885

  19. Treatment and prevention of infection following bites of the Antarctic fur seal (Arctocephalus gazella).

    PubMed

    Kouliev, Timur; Cui, Victoria

    2015-01-01

    In recent decades, an increasing number of people have traveled to sub-Antarctic and Antarctic regions each year for research, tourism, and resource exploitation. Hunting of Antarctic fur seals (Arctocephalus gazella) almost pushed the species to extinction in the early 1900s, but populations have since shown rapid and substantial recovery. The species' range has re-expanded to include several islands south of the Antarctic Convergence, most notably South Georgia, and now overlaps with many popular Antarctic travel destinations. Both male and female fur seals can become extremely aggressive when provoked, and their bites, if not properly treated, pose a significant risk of infection by microorganisms not usually encountered in cases of animal bites. In this report, we present the case of a patient treated for a fur seal bite during an Antarctic expedition cruise, review the literature concerning seal bites, and suggest the use of antibiotic prophylaxis to prevent complications.

  20. Molecular Detection of Circovirus and Adenovirus in Feces of Fur Seals (Arctocephalus spp.).

    PubMed

    Chiappetta, Catarina Marcon; Cibulski, Samuel Paulo; Lima, Francisco Esmaile Sales; Varela, Ana Paula Muterle; Amorim, Derek Blaese; Tavares, Maurício; Roehe, Paulo Michel

    2017-03-01

    In some regions, little is known about exposure to viruses in coastal marine mammals. The present study aimed to detect viral RNA or DNA in 23 free-ranging fur seals on the northern coastline of Rio Grande do Sul State, Brazil. Polymerase chain reaction was used to detect nucleic acids of circoviruses, adenoviruses, morbilliviruses, vesiviruses, and coronaviruses in the feces from twenty-one South American fur seals (Arctocephalus australis) and two Subantarctic fur seals (A. tropicalis). Adenovirus DNA fragments were detected in two South American fur seals; nucleotide sequences of these fragments revealed a high degree of similarity to human adenovirus type C. Circovirus DNA fragments were detected in six animals of the same species. Two were phylogenetically similar to the Circovirus genus, whereas the other four nucleotide fragments showed no similarity to any of the known genera within the family Circoviridae. RNA fragments indicating the presence of coronavirus, vesivirus, and morbillivirus were not detected. These findings suggest that adenoviruses and circoviruses are circulating in fur seal populations found along the coast of Rio Grande do Sul State, Brazil.

  1. Activity Budgets of Captive Cape Fur Seals (Arctocephalus pusillus) Under a Training Regime.

    PubMed

    Wierucka, Kaja; Siemianowska, Sonia; Woźniak, Marta; Jasnosz, Katarzyna; Kieliszczyk, Magdalena; Kozak, Paulina; Sergiel, Agnieszka

    2016-01-01

    Ethograms and time budgets are crucial for the behavioral assessment of nonhuman animals in zoos, and they serve as references for welfare research. This study was conducted to obtain detailed time budgets of trained Cape fur seals (Arctocephalus pusillus) in captivity, to evaluate variations of these patterns, and to determine whether abnormal behaviors had been displayed. Behavioral data for 3 Cape fur seals in the Wroclaw Zoo were collected, and more than 300 observation hours (during a 12-month period) per individual were analyzed. The studied animals exhibited a diversified repertoire of natural behaviors with apparent seasonal and daily patterns, and they did not present stereotypic behaviors. Significant differences of interaction rates between individuals suggest more frequent affiliative interactions among related animals. The absence of stereotypic behaviors, good health of individuals, and the presence of diversified natural behaviors indicated relatively good welfare of Cape fur seals kept in the Wroclaw Zoo.

  2. Characteristics of marine debris that entangle Australian fur seals (Arctocephalus pusillus doriferus) in southern Australia.

    PubMed

    Lawson, T J; Wilcox, Chris; Johns, Karen; Dann, P; Hardesty, Britta Denise

    2015-09-15

    Marine debris is a global issue that can have devastating impacts on marine mammals. To understand the types of materials that result in entanglement and thus the potential impact of entangling items on marine wildlife, we analysed data collected from items in which Australian fur seals had been entangled in southern Victoria, Australia over a 15year period. From 1997 to 2012, 138 entangling items were removed from seals. The majority of these entanglements were plastic twine or rope, and seals were entangled in green items more than in any other colour. In general, younger seals were more likely to be entangled than adults. Understanding the effects of marine debris entanglement on the Australian fur seal population can lead to more effective management of the sources of debris and the wildlife that interact with it.

  3. Mercury concentrations in the Australian fur seal Arctocephalus pusillus from SE Australian waters

    SciTech Connect

    Bacher, G.J.

    1985-10-01

    Marine carnivores such as seals and sea lions occupy an important position in the upper trophic level of the marine food web and this, together with their longevity, makes these marine mammals useful indicators of mercury accumulation in the marine environment. Little information exists on mercury concentrations in marine mammals from the southern hemisphere. This paper reports total mercury concentrations in the tissues of the Australian Fur Seal Arctocephalus pusillus from southeastern Australian waters.

  4. 50 CFR 223.201 - Guadalupe fur seal.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... prohibitions of section 9 of the Act (16 U.S.C. 1538) relating to endangered species apply to the Guadalupe fur... accomplished in a humane manner; (ii) Is for the protection or welfare of the animal, is for the protection of... steps designed to ensure the return of the animal to its natural habitat, if feasible; and (iv)...

  5. 76 FR 45499 - Marine Mammals; Subsistence Taking of Northern Fur Seals; Harvest Estimates

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-29

    ... season for herd conservation. To further minimize negative effects on the Pribilof Islands' fur seal... have been discussed with each tribal government as part of the co-management relationship and agreement... reasons. Weather conditions and availability of animals varies annually. The availability of wage...

  6. Olfactory discrimination ability of South African fur seals (Arctocephalus pusillus) for enantiomers.

    PubMed

    Kim, Sunghee; Amundin, Mats; Laska, Matthias

    2013-06-01

    Using a food-rewarded two-choice instrumental conditioning paradigm we assessed the ability of South African fur seals, Arctocephalus pusillus, to discriminate between 12 enantiomeric odor pairs. The results demonstrate that the fur seals as a group were able to discriminate between the optical isomers of carvone, dihydrocarvone, dihydrocarveol, menthol, limonene oxide, α-pinene, fenchone (all p < 0.01), and β-citronellol (p < 0.05), whereas they failed to distinguish between the (+)- and (-)-forms of limonene, isopulegol, rose oxide, and camphor (all p > 0.05). An analysis of odor structure-activity relationships suggests that a combination of molecular structural properties rather than a single molecular feature may be responsible for the discriminability of enantiomeric odor pairs. A comparison between the discrimination performance of the fur seals and that of other species tested previously on the same set of enantiomers (or subsets thereof) suggests that the olfactory discrimination capabilities of this marine mammal are surprisingly well developed and not generally inferior to that of terrestrial mammals such as human subjects and non-human primates. Further, comparisons suggest that neither the relative nor the absolute size of the olfactory bulbs appear to be reliable predictors of between-species differences in olfactory discrimination capabilities. Taken together, the results of the present study support the notion that the sense of smell may play an important and hitherto underestimated role in regulating the behavior of fur seals.

  7. Successful acquisition of an olfactory discrimination paradigm by South African fur seals, Arctocephalus pusillus.

    PubMed

    Laska, Matthias; Svelander, Madeleine; Amundin, Mats

    2008-03-18

    The present study demonstrates that South African fur seals, Arctocephalus pusillus, can successfully be trained to discriminate between objects on the basis of odor cues. Using a task based on a food-rewarded two-choice discrimination of simultaneously presented odor stimuli the animals acquired the basic operant conditioning paradigm within 480 to 880 stimulus contacts. Moreover, the fur seals could readily transfer to new S+ and S- stimuli, were capable of distinguishing between fish- and non-fish odors as well as between two fish odors, and were able to remember the reward value of previously learned odor stimuli even after 2- and 15-week breaks. The precision and consistency of the fur seals' performance in tests of discrimination ability and memory demonstrate the suitability of this paradigm for assessing olfactory function in this pinniped. An across-species comparison of several measures of olfactory learning capabilities such as speed of initial task acquisition and ability to master transfer tasks shows that A. pusillus is similar in performance to non-human primates, but inferior to rodents such as mice and rats. The results support the assumption that fur seals may use olfactory cues for social communication and food selection and that the sense of smell may play an hitherto underestimated role in the control of their behavior.

  8. Characterization of the faecal bacterial community of wild young South American (Arctocephalus australis) and Subantarctic fur seals (Arctocephalus tropicalis).

    PubMed

    Medeiros, Aline Weber; Giongo, Adriana; Valdez, Fernanda P; Blaese de Amorin, Derek; Tavares, Maurício; d'Azevedo, Pedro A; Franco, Ana Claudia; Frazzon, Jeverson; Frazzon, Ana P G

    2016-03-01

    The microbiota of wild marine mammals is poorly understood, perhaps due to the migratory habits of some species and the difficulty in obtaining samples. Using high-throughput sequencing, the present study examines the faecal bacterial community of wild young South American (Arctocephalus australis) and Subantarctic fur seals (A. tropicalis). Faecal samples from South American (n = 6) and Subantarctic fur seals (n = 4) found dead along the south coast of Brazil were collected. Sequences were assigned to taxa using the Ribosomal Database Project-Bayesian classifier. Diversity of the microbiota was assessed by categorization of sequence reads into operational taxonomic units. Results indicate that Firmicutes (88.556%-84.016%) was the predominant phylum in South American and Subantarctic fur seals. The distribution of Actinobacteria and Proteobacteria varied according to the fur seal species. Fusobacteria and Bacteroidetes represented less than 1% of the sequences. The most abundant order in both fur seals was Clostridiales (88.64% and 87.49%). Individual variable incidences were observed in the composition of family among the fur seals, though the families Lachnospiraceae, Peptostreptococcaceae, Ruminococcaceae and Coriobacteriaceae were more prevalent. This study provides insight into the faecal bacterial community of wild young South American and Subantarctic fur seals.

  9. Mitochondrial DNA preservation across 3000-year-old northern fur seal ribs is not related to bone density: Implications for forensic investigations.

    PubMed

    Barta, Jodi Lynn; Monroe, Cara; Crockford, Susan J; Kemp, Brian M

    2014-06-01

    While recent forensic research has focused on determining which skeletal elements are superior in their preservation of DNA over the long term, little focus has been placed on measuring intra-element variation. Moreover, there is a general belief that dense (cortical) bone material will contain better-preserved DNA than does spongy (cancellous) bone. To address these ideas, quantitative PCR was used to estimate the degree of mitochondrial DNA (mtDNA) preservation variance across sections of 19 northern fur seal ribs (Callorhinus ursinus) that date to ∼3000 years before present. Further, we developed a measure called the "density index" that was used to gauge the relative densities of the rib sections studied here to determine if density was an appropriate predictor of preservation. The average preservation among the samples was significantly different (ANOVA, p=1.9×10(-9)) with only 15% of the total variance observed within samples. However, 12 of the 19 specimens (∼63.2%) exhibited at least an order of magnitude difference in mtDNA preservation across the whole. Regression of the amount of mtDNA extracted per gram of bone material against the density index of the bone from which it was extracted demonstrates no relationship between these variables (R(2)=0.03, p=0.28).

  10. Polychlorinated biphenyls and organochlorine pesticides in the Australian fur seal, Arctocephalus pusillus doriferus

    SciTech Connect

    Smillie, R.H.; Waid, J.S.

    1987-08-01

    Seals occupy a high trophic level in the marine ecosystem and have large reserves of subcutaneous fats. It is, therefore, not surprising that they accumulate high concentration of lipophilic substances such as organochlorine pesticides. Seal tissue concentration of pesticides are considered by some workers to be a good indication of local environmental loads. The Australian fur seals at Seal Rocks occupy a breading colony from which they can range into Bass Strait, Westernport Bay and Port Phillip Bay. It is, therefore, reasonable that results obtained for local seals should provide an indication of pesticides and PCBs in local squid and schooling fish. Previous results indicate that biota in Port Phillip Bay and Bass Strait, adjacent to the colony are exposed to elevated concentrations of PCBs. The authors have determined PCB and pesticide levels in local seals.

  11. Regional differences in plastic ingestion among Southern Ocean fur seals and albatrosses.

    PubMed

    Ryan, Peter G; de Bruyn, P J Nico; Bester, Marthán N

    2016-03-15

    We provide data on regional differences in plastic ingestion for two Southern Ocean top predators: Arctocephalus fur seals and albatrosses (Diomedeidae). Fur seals breeding on Macquarie Island in the 1990s excreted small (mainly 2-5 mm) plastic fragments, probably derived secondarily from myctophid fish. No plastic was found in the scats of these seals breeding on three islands in the southwest Indian and central South Atlantic Oceans, despite myctophids dominating their diets at these locations. Compared to recent reports of plastic ingestion by albatrosses off the east coast of South America, we confirm that plastic is seldom found in the stomachs of Thalassarche albatrosses off South Africa, but found no Diomedea albatrosses to contain plastic, compared to 26% off South America. The reasons for such regional differences are unclear, but emphasize the importance of reporting negative as well as positive records of plastic ingestion by marine biota.

  12. Perfluorinated contaminants in fur seal pups and penguin eggs from South Shetland, Antarctica.

    PubMed

    Schiavone, A; Corsolini, S; Kannan, K; Tao, L; Trivelpiece, W; Torres, D; Focardi, S

    2009-06-01

    Perfluorinated compounds (PFCs) have emerged as a new class of global environmental pollutants. In this study, the presence of perfluorochemicals (PFCs) in penguin eggs and Antarctic fur seals was reported for the first time. Tissue samples from Antarctic fur seal pups and penguin eggs were collected during the 2003/04 breeding season. Ten PFC contaminants were determined in seal and penguin samples. The PFC concentrations in seal liver were in the decreasing order, PFOS>PFNA>PFHpA>PFUnDA while in Adélie penguin eggs were PFHpA>PFUnDA>PFDA>PFDoDA, and in Gentoo penguin eggs were PFUnDA>PFOS>PFDoDA>PFHpA. The PFC concentrations differed significantly between seals and penguins (p<0.005) and a species-specific difference was found between the two species of penguins (p<0.005). In our study we found a mean concentration of PFOS in seal muscle and liver samples of 1.3 ng/g and 9.4 ng/g wet wt, respectively, and a mean concentration in Gentoo and Adélie penguin eggs of 0.3 ng/g and 0.38 ng/g wet wt, respectively. PFCs detected in penguin eggs and seal pups suggested oviparous and viviparous transfer of PFOS to eggs and off-springs.

  13. Case 3058. Arctocephalus F. Cuvier, 1826 and Callorhinus Gray, 1859 (Mammalia, Pinnipedia): proposed conservation by the designation of Phoca pusilla Schreber, [1775] as the type species of Arctocephalus; and Otaria Peron, 1816 and Eumetopias Gill, 1866: proposed conservation by the designation of Phoca leonina Molina, 1782 as the type species of Otaria

    USGS Publications Warehouse

    Gardner, A.L.; Robbins, C.B.

    1999-01-01

    The purpose of this application is to conserve the accustomed understanding and usage of the fur seal name Arctocephalus F. Cuvier, 1826 by the designation of Phoca pusilia Schreber, [1775] as the type species, thus conserving also the name Callorhinus Gray, 1859. At present Phoca ursina Linnaeus, 1758 is the valid type species of both Arctocephalus and Callorhinus. The name Arctocephalus relates to a genus of some seven fur seals from the southern hemisphere, while Callorhinus is used for the single species C. ursinus (Linnaeus) from the northern hemisphere. It is also proposed that the universal understanding of the names Otaria Peron, 1816 and Eumetopias Gill, 1866 should be conserved for the southern and northern sea lions respectively by designating Phoca leonina Molina, 1782 (for which the valid specific name is P. byronia de Blainville, 1820) as the type species of Otaria. At present Phoca jubata Schreber, [1776] is the type species of Otaria and the name Otaria is a senior subjective synonym of Eumetopias. The four genera Arctocephalus, Callorhinus, Otaria and Eumetopias are all placed in the family OTARIIDAE Gray, 1825.

  14. The fat and the furriest: morphological changes in harp seal fur with ontogeny.

    PubMed

    Gmuca, Natalia V; Pearson, Linnea E; Burns, Jennifer M; Liwanag, Heather E M

    2015-01-01

    Ontogenetic changes in physiological performance often exemplify the development of adaptations to environmental challenges. For mammals in polar regions, the extreme cold of the environment presents a constant challenge to thermal homeostasis. The harp seal (Pagophilus groenlandicus) is an Arctic species that shifts its thermoregulatory strategy with ontogeny. Adult harp seals primarily use blubber for insulation, but newborn harp seals instead rely on their fur coat while their blubber layer develops. Harp seal pups are weaned abruptly, less than 2 wk after birth, and must subsequently learn to swim and dive in frigid waters on their own. This study examined how the morphological characteristics of harp seal fur change with ontogeny. We compared hair length, hair circularity, and hair density for neonates (1 d old; n = 7), early-nursing pups (4 d old; n = 3), late-nursing pups (9 d old; n = 4), newly weaned (molting) pups (2 wk old; n = 5), late-weaned (molted) pups (3 wk old; n = 4), and adult harp seals (n = 4). Hairs were shorter (P < 0.001) and flatter (P < 0.001) in older animals. Additionally, hair density decreased with age (P < 0.001), in terms of both the average number of hair bundles per unit area and the average number of underhairs present in any given bundle. These morphological changes were associated with a reduced thermal resistance of the pelt in late-weaned (molted) pups and adults (P < 0.001). Results are consistent with known evolutionary patterns of fur morphology associated with the transition from fur to blubber in aquatic species, yet this is the first time such morphological differences have been demonstrated across age classes within a single species. Thus, the ontogenetic patterns described here for harp seals recapitulate the convergent phylogenetic patterns observed across secondarily aquatic species. Overall, the timing of these ontogenetic changes may limit the ability of harp seals to adapt to the deterioration of sea ice in the

  15. Antimicrobial resistance and virulence factor gene profiles of Enterococcus spp. isolates from wild Arctocephalus australis (South American fur seal) and Arctocephalus tropicalis (Subantarctic fur seal).

    PubMed

    Santestevan, Naiara Aguiar; de Angelis Zvoboda, Dejoara; Prichula, Janira; Pereira, Rebeca Inhoque; Wachholz, Guilherme Raffo; Cardoso, Leonardo Almansa; de Moura, Tiane Martin; Medeiros, Aline Weber; de Amorin, Derek Blaese; Tavares, Maurício; d'Azevedo, Pedro Alves; Franco, Ana Claudia; Frazzon, Jeverson; Frazzon, Ana Paula Guedes

    2015-12-01

    Enterococci are natural inhabitants of the gastrointestinal tracts in humans and animals. Epidemiological data suggest that enterococci are important reservoirs of antimicrobial resistant genes that may be transmitted from other bacterial species The aim of this study was to investigate the species composition, antimicrobial resistance and virulence genes in enterococci recovered from fecal samples of wild Arctocephalus australis and A. tropicalis found dead along the South Coast of Brazil. From a total of 43 wild fur seals, eleven were selected for this study. Phenotypic and genotypic characterizations were used to classify Enterococcus species. Strains were tested for susceptibility to 10 antibiotics, presence of ace, gelE, asa, cylA, tet(L), tet(M) and erm(B) genes by PCR, and genetic variability using RAPD-PCR. Among the 50 enterococci isolated, 40% were Enterococcus faecalis, 40% E. hirae, 12% E. casseliflavus and 8 % other enterococcal species. Resistance profiles were observed to erythromycin, nitrofurantoin, tetracycline, norfloxacin and ciprofloxacin. The prevalence of virulence genes was ace (68%), gelE (54%), asa (22%) and cylA (4%). In erythromycin- and tetracycline strains, erm(B) and tet(M) were detected, respectively. The RAPD-PCR demonstrated a close phylogenetic relationship between the enterococci isolated from A. australis and A. tropicalis. In conclusion, different enterococcus species showing antimicrobial resistance and virulence determinates were isolated from fecal samples of fur seals. Antibiotic resistant strains in these animals could be related within food chain and aquatic pollutants or linked to environmental resistome, and demonstrates the potential importance of these animals as reservoirs and disseminators of such determinants in marine environmental.

  16. Foraging-Based Enrichment Promotes More Varied Behaviour in Captive Australian Fur Seals (Arctocephalus pusillus doriferus)

    PubMed Central

    Hocking, David P.; Salverson, Marcia; Evans, Alistair R.

    2015-01-01

    During wild foraging, Australian fur seals (Arctocephalus pusillus doriferus) encounter many different types of prey in a wide range of scenarios, yet in captive environments they are typically provided with a narrower range of opportunities to display their full repertoire of behaviours. This study aimed to quantitatively explore the effect of foraging-based enrichment on the behaviour and activity patterns displayed by two captive Australian fur seals at Melbourne Zoo, Australia. Food was presented as a scatter in open water, in a free-floating ball device, or in a static box device, with each treatment separated by control trials with no enrichment. Both subjects spent more time interacting with the ball and static box devices than the scatter feed. The total time spent pattern swimming was reduced in the enrichment treatments compared to the controls, while the time spent performing random swimming behaviours increased. There was also a significant increase in the total number of bouts of behaviour performed in all three enrichment treatments compared to controls. Each enrichment method also promoted a different suit of foraging behaviours. Hence, rather than choosing one method, the most effective way to increase the diversity of foraging behaviours, while also increasing variation in general activity patterns, is to provide seals with a wide range of foraging scenarios where food is encountered in different ways. PMID:25946412

  17. The influence of preceding dive cycles on the foraging decisions of Antarctic fur seals

    PubMed Central

    Iwata, T.; Sakamoto, K. Q.; Edwards, E. W. J.; Staniland, I. J.; Trathan, P. N.; Goto, Y.; Sato, K.; Naito, Y.; Takahashi, A.

    2015-01-01

    The foraging strategy of many animals is thought to be determined by their past experiences. However, few empirical studies have investigated whether this is true in diving animals. We recorded three-dimensional movements and mouth-opening events from three Antarctic fur seals during their foraging trips to examine how they adapt their behaviour based on past experience—continuing to search for prey in the same area or moving to search in a different place. Each dive cycle was divided into a transit phase and a feeding phase. The linear horizontal distance travelled after feeding phases in each dive was affected by the mouth-opening rate during the previous 244 s, which typically covered two to three dive cycles. The linear distance travelled tended to be shorter when the mouth-opening rate in the previous 244 s was higher, i.e. seals tended to stay in the same areas with high prey-encounter rates. These results indicate that Antarctic fur seals follow decision-making strategies based on the past foraging experience over time periods longer than the immediately preceding dive. PMID:26156132

  18. A Bayesian hierarchical model of Antarctic fur seal foraging and pup growth related to sea ice and prey abundance.

    PubMed

    Hiruki-Raring, Lisa M; Ver Hoef, Jay M; Boveng, Peter L; Bengtson, John L

    2012-03-01

    We created a Bayesian hierarchical model (BHM) to investigate ecosystem relationships between the physical ecosystem (sea ice extent), a prey measure (krill density), predator behaviors (diving and foraging effort of female Antarctic fur seals, Arctocephalus gazella, with pups) and predator characteristics (mass of maternal fur seals and pups). We collected data on Antarctic fur seals from 1987/1988 to 1994/1995 at Seal Island, Antarctica. The BHM allowed us to link together predators and prey into a model that uses all the data efficiently and accounts for major sources of uncertainty. Based on the literature, we made hypotheses about the relationships in the model, which we compared with the model outcome after fitting the BHM. For each BHM parameter, we calculated the mean of the posterior density and the 95% credible interval. Our model confirmed others' findings that increased sea ice was related to increased krill density. Higher krill density led to reduced dive intensity of maternal fur seals, as measured by dive depth and duration, and to less time spent foraging by maternal fur seals. Heavier maternal fur seals and lower maternal foraging effort resulted in heavier pups at 22 d. No relationship was found between krill density and maternal mass, or between maternal mass and foraging effort on pup growth rates between 22 and 85 days of age. Maternal mass may have reflected environmental conditions prior to the pup provisioning season, rather than summer prey densities. Maternal mass and foraging effort were not related to pup growth rates between 22 and 85 d, possibly indicating that food was not limiting, food sources other than krill were being used, or differences occurred before pups reached age 22 d.

  19. [The parallelisms in of sound signal of domestic sheep and Northern fur seals].

    PubMed

    Nikol'skiĭ, A A; Lisitsina, T Iu

    2011-01-01

    The parallelisms in communicative behavior of domestic sheep and Northern fur seals within a herd are accompanied by parallelisms in parameters of sound signal, the calling scream. This signal ensures ties between babies and their mothers at a long distance. The basis of parallelisms is formed by amplitude modulation at two levels: the one being a direct amplitude modulation of the carrier frequency and the other--modulation of the carrier frequency oscillation. Parallelisms in the signal oscillatory process result in corresponding parallelisms in the structure of its frequency spectrum.

  20. The endocrine pancreas of the Cape fur seal, Arctocephalus pusillus (Schreber, 1776): an immunocytochemical study.

    PubMed

    Erasmus, C P; Van Aswegen, G

    1997-09-01

    The indirect peroxidase method was employed to study the endocrine pancreas of the Cape fur seal. Immunoreactivity to insulin was confined to the cores of the islets and the insulin cells were more abundant than the other endocrine cell types, which occurred mainly in the mantles of the islets. Of these, glucagon cells were the most numerous, followed by somatostatin and pancreatic polypeptide (PP) cells. The latter were observed in the mantles of the islets and scattered in the exocrine tissue of the duodenal lobe. The marked variation in the shape and the distribution of the endocrine cells in the mantles of the islets seen in the pancreas of the seal, seems to be typical of carnivorous species like the cat and dog.

  1. Origins and biological accumulation of small plastic particles in fur seals from Macquarie Island.

    PubMed

    Eriksson, Cecilia; Burton, Harry

    2003-09-01

    One hundred and sixty four plastic particles (mean length 4.1 mm) recovered from the scats of fur seals (Arctocephalus spp.) on Macquarie Island were examined. Electron micrographs of 41 of the plastic particles showed that none could be identified as plastic pellet feedstock from their shapes. Commonly, such pellets are cylindrical and spherical. Instead, all the 164 plastic particles from the seal scats were angular particles of 7 colors (feedstock particles are normally opaque or white) and could be classified into 2 categories: i) fragmented along crystal lines and likely to be the result of UV breakdown; and ii) worn by abrasion (where striations were clearly visible) into irregular shapes with rounded corners. White, brown, green, yellow and blue were the most common colors. In composition, they came from 5 polymer groups; polyethylene 93%, polypropylene 4%, poly(1-Cl-1-butenylene) polychloroprene 2%, melamine-urea (phenol) (formaldehyde) resin 0.5%, and cellulose (rope fiber) 0.5%. The larger groups are buoyant with a specific gravity less than that of seawater. These small plastic particles are formed from the breakdown of larger particles (fragments). Their origin seems to be from the breakdown of user plastics washed ashore and ground down on cobbled beaches. Certainly most particles (70%) had attained their final form by active abrasion. It is hypothesized that the plastic particles were washed out to sea and then selected by size and consumed by individuals of a pelagic fish species, Electrona subaspera, who in turn were consumed by the fur seals. Thus, the particles were accumulated both by the fish and the seals in the usual process of their feeding.

  2. Colony-level assessment of Brucella and Leptospira in the Guadalupe fur seal, Isla Guadalupe, Mexico.

    PubMed

    Ziehl-Quirós, E Carolina; García-Aguilar, María C; Mellink, Eric

    2017-01-24

    The relatively small population size and restricted distribution of the Guadalupe fur seal Arctocephalus townsendi could make it highly vulnerable to infectious diseases. We performed a colony-level assessment in this species of the prevalence and presence of Brucella spp. and Leptospira spp., pathogenic bacteria that have been reported in several pinniped species worldwide. Forty-six serum samples were collected in 2014 from pups at Isla Guadalupe, the only place where the species effectively reproduces. Samples were tested for Brucella using 3 consecutive serological tests, and for Leptospira using the microscopic agglutination test. For each bacterium, a Bayesian approach was used to estimate prevalence to exposure, and an epidemiological model was used to test the null hypothesis that the bacterium was present in the colony. No serum sample tested positive for Brucella, and the statistical analyses concluded that the colony was bacterium-free with a 96.3% confidence level. However, a Brucella surveillance program would be highly recommendable. Twelve samples were positive (titers 1:50) to 1 or more serovars of Leptospira. The prevalence was calculated at 27.1% (95% credible interval: 15.6-40.3%), and the posterior analyses indicated that the colony was not Leptospira-free with a 100% confidence level. Serovars Icterohaemorrhagiae, Canicola, and Bratislava were detected, but only further research can unveil whether they affect the fur seal population.

  3. Terrestrial apnoeas and the development of cardiac control in Australian fur seal (Arctocephalus pusillus doriferus) pups.

    PubMed

    Deacon, N L; Arnould, J P Y

    2009-04-01

    The development of cardiac control in association with terrestrial respiration patterns was examined throughout the period of maternal dependence in Australian fur seal pups. Resting eupnoic heart rate and respiration rate were significantly correlated (r (2) = 0.49) and both decreased with age (P < 0.05 in both cases). From an early age (1 month), pups displayed terrestrial apnoeas (18.1 +/- 0.5 s) accompanied by substantial bradycardia (127 beats min(-1), a 13% decrease from eupnoic HR). Terrestrial apnoea duration increased significantly with age reaching a mean of 41 s just prior to weaning, slightly lower than the mean dive duration (52 s) previously recorded for pups of the same age. Correspondingly, mean apnoic heart rate decreased with age to 74 beats min(-1) just prior to weaning, representing a 25% decrease on eupnoic heart rate. Importantly, concomitant with the decrease in mean apnoic heart rate with age, an increase in the control of bradycardia was evident with the variability in instantaneous apnoic heart decreasing such that older pups were able to maintain a low steady heart rate for the duration of the apnoea. The changes seen in these parameters are similar to those reported during postnatal development in elephant seals (Mirounga spp.) and harbour seals (Phoca vitulina), and are considered indicative of the development of cardiac control. These findings suggest a common strategy for the development of bradycardia control in both otariid and phocid seals.

  4. Measurement of ocean temperatures using instruments carried by Antarctic fur seals

    NASA Astrophysics Data System (ADS)

    Boyd, I. L.; Hawker, E. J.; Brandon, M. A.; Staniland, I. J.

    2001-01-01

    The study aimed to test the utility of instruments deployed on marine mammals for measuring physical oceanographic variation and, using this method, to examine temperature variation in the coastal waters around South Georgia. There was a significant correlation between temperature measurements made using a towed undulating oceanographic recorder (UOR) and concurrent measurements from time-depth recorders (TDRs) fitted to lactating Antarctic fur seals foraging from the coast of South Georgia. Congruence was found at horizontal spatial scales from 0.01°×0.01° to 0.5°×0.5° (degrees of latitude and longitude), and at a vertical scale of 10 m. However, there was no significant correlation between temperature measured by TDRs in the top 5 m and sea surface temperature (SST) measured by satellite remote sensing. TDR data provided information about temperature variation vertically through the water column, and through time. The UOR data were used to recalibrate the TDR data in order to correct for the slow response time of the TDR thermistor relative to the speed of seal movements through the water column. Seasonal temperature variation was apparent, and temperatures also varied between regions, and with bathymetry. These results were consistent with the current interpretation of the coastal oceanography around South Georgia. In particular, the relationship between on- and off-shelf waters showed larger amounts of warmer surface water in a region in which more run-off was to be expected. The study also showed that Antarctic fur seals concentrate their activity in regions of colder, and presumably oceanic, water. Such instrumented animals could provide near real time data for assimilation into ocean models.

  5. Early-life sexual segregation: ontogeny of isotopic niche differentiation in the Antarctic fur seal

    NASA Astrophysics Data System (ADS)

    Kernaléguen, L.; Arnould, J. P. Y.; Guinet, C.; Cazelles, B.; Richard, P.; Cherel, Y.

    2016-09-01

    Investigating the ontogeny of niche differentiation enables to determine at which life-stages sexual segregation arises, providing insights into the main factors driving resource partitioning. We investigated the ontogeny of foraging ecology in Antarctic fur seals (Arctocephalus gazella), a highly dimorphic species with contrasting breeding strategies between sexes. Sequential δ13C and δ15N values of whiskers provided a longitudinal proxy of the foraging niche throughout the whole life of seals, from weaning, when size dimorphism is minimal to the age of 5. Females exhibited an early-life ontogenetic shift, from a total segregation during their first year at-sea, to a similar isotopic niche as breeding females as early as age 2. In contrast, males showed a progressive change in isotopic niche throughout their development such that 5-year-old males did not share the same niche as territorial bulls. Interestingly, males and females segregated straight after weaning with males appearing to feed in more southerly habitats than females. This spatial segregation was of similar amplitude as observed in breeding adults and was maintained throughout development. Such early-life niche differentiation is an unusual pattern and indicates size dimorphism and breeding constraints do not directly drive sexual segregation contrary to what has been assumed in otariid seals.

  6. Effects of the presence of official-looking volunteers on harassment of New Zealand fur seals.

    PubMed

    Acevedo-Gutiérrez, Alejandro; Acevedo, Lisa; Boren, Laura

    2011-06-01

    An increased number of tourists viewing animals in the wild have increased stress on these animals (hereafter wildlife). Many wildlife-viewing locations rely on voluntary compliance with posted regulations to protect animals from tourists because of the expense of employing on-site enforcement personnel. Voluntary compliance, however, is ineffective. The presence of official-looking volunteers may decrease the incidence of wildlife harassment by tourists. To test this possibility, we observed tourists interacting with 5- to 12-month-old New Zealand fur seals (Arctocephalus forsteri) at the popular Ohau Stream waterfall while in the absence or presence of a young woman in plain sight wearing a neon vest (i.e., observer) and when an observer was not present. We observed 254 tourist groups at the waterfall when young seals were present. The percentage of groups in which at least one person harassed (approached, touched, or threw objects) a young seal was two-thirds lower when the official-looking observer was present. Frequency of harassment was inversely related to observer presence. Programs in which volunteers work at tourist sites are popular in countries with high tourism rates, such as New Zealand. Our results show that a relatively inexpensive and effective tourism-management strategy may be to post such volunteers as observers at sites where tourists view wildlife.

  7. Early-life sexual segregation: ontogeny of isotopic niche differentiation in the Antarctic fur seal

    PubMed Central

    Kernaléguen, L.; Arnould, J. P. Y.; Guinet, C.; Cazelles, B.; Richard, P.; Cherel, Y.

    2016-01-01

    Investigating the ontogeny of niche differentiation enables to determine at which life-stages sexual segregation arises, providing insights into the main factors driving resource partitioning. We investigated the ontogeny of foraging ecology in Antarctic fur seals (Arctocephalus gazella), a highly dimorphic species with contrasting breeding strategies between sexes. Sequential δ13C and δ15N values of whiskers provided a longitudinal proxy of the foraging niche throughout the whole life of seals, from weaning, when size dimorphism is minimal to the age of 5. Females exhibited an early-life ontogenetic shift, from a total segregation during their first year at-sea, to a similar isotopic niche as breeding females as early as age 2. In contrast, males showed a progressive change in isotopic niche throughout their development such that 5-year-old males did not share the same niche as territorial bulls. Interestingly, males and females segregated straight after weaning with males appearing to feed in more southerly habitats than females. This spatial segregation was of similar amplitude as observed in breeding adults and was maintained throughout development. Such early-life niche differentiation is an unusual pattern and indicates size dimorphism and breeding constraints do not directly drive sexual segregation contrary to what has been assumed in otariid seals. PMID:27620663

  8. Mating success and body condition not related to foraging specializations in male fur seals

    PubMed Central

    Cherel, Y.; Guinet, C.; Arnould, J. P. Y.

    2016-01-01

    Individual specialization is widespread among wild populations. While its fitness consequences are central in predicting the ecological and evolutionary trajectories of populations, they remain poorly understood. Long-term individual foraging specializations occur in male Antarctic (Arctocephalus gazella) and Australian (A. pusillus doriferus) fur seals. Strong selective pressure is expected in these highly dimorphic and polygynous species, raising the question of the fitness payoffs associated with different foraging strategies. We investigated the relationship between individual isotopic niche (a proxy of foraging specialization), body size and condition, and an index of reproductive success (harem size) in territorial males. Individuals varied greatly in their skin and fur isotopic values reflecting a range of foraging strategies within the two populations. However, in both species, isotopic niche was not correlated to body size, condition or mating success (R2/ρ < 0.06). Furthermore, no foraging niche was predominant in either species, which would have indicated a substantial long-term fitness benefit of a particular strategy via a higher survival rate. These results suggest that the fitness consequences of a foraging strategy depend not only on the quality of prey and feeding habitat but also on an individual's hunting efficiency and skills. PMID:27493771

  9. Chemical fingerprints encode mother–offspring similarity, colony membership, relatedness, and genetic quality in fur seals

    PubMed Central

    Stoffel, Martin A.; Caspers, Barbara A.; Forcada, Jaume; Giannakara, Athina; Baier, Markus; Eberhart-Phillips, Luke; Müller, Caroline; Hoffman, Joseph I.

    2015-01-01

    Chemical communication underpins virtually all aspects of vertebrate social life, yet remains poorly understood because of its highly complex mechanistic basis. We therefore used chemical fingerprinting of skin swabs and genetic analysis to explore the chemical cues that may underlie mother–offspring recognition in colonially breeding Antarctic fur seals. By sampling mother–offspring pairs from two different colonies, using a variety of statistical approaches and genotyping a large panel of microsatellite loci, we show that colony membership, mother–offspring similarity, heterozygosity, and genetic relatedness are all chemically encoded. Moreover, chemical similarity between mothers and offspring reflects a combination of genetic and environmental influences, the former partly encoded by substances resembling known pheromones. Our findings reveal the diversity of information contained within chemical fingerprints and have implications for understanding mother–offspring communication, kin recognition, and mate choice. PMID:26261311

  10. Reproductive Tract Histology in South American Fur Seal Pups (Arctophoca australis).

    PubMed

    Katz, Helena; Johansson, Olle

    2017-03-01

    In the present study, a detailed histological description of the female reproductive tract of South American fur seal (Arctophoca australis) pups has been conducted. The uterine tube was covered by cuboidal to columnar epithelium; nerve fibers were present in the mesosalpinx and beneath the muscular layer. The uterus was bipartitus; the endometrial surface of the horns was lined by a simple cuboidal or columnar epithelium with deep tubular glands; caudally ("the transition area"), the epithelium changed to pseudostratified columnar, few tubular glands were present and the myometrium increased in width. A bistratified epithelium internally coated the uterine body, whereas it changed to cylindrical stratified epithelium with a highly vascularized lamina propria and a strong muscular layer in the cervix; no endometrial glands were observed in this region. From the transition area of the uterus to the vagina there were several nerve fibers and ganglia belonging to the uterovaginalis plexus. In the vestibule, hymenal folds were poorly developed; adnexa structures included the major vestibular glands and a neurovascular structure similar to the vestibular bulb. Minor vestibular glands were associated with the clitoris. The skin of the perineum was lined by a keratinized stratified epithelium, pigmented, with sebaceous glands, sweat glands and hair follicles. This is the first detailed histological description of the reproductive tract of South American fur seal pups, including the glandular adnexa and nerve structures. These results contribute to the reproductive biology in Pinniped species, and give a better understanding of the utero-placental perfusion mechanism during diving. Anat Rec, 2017. © 2017 Wiley Periodicals, Inc. Anat Rec, 300:600-613, 2017. © 2016 Wiley Periodicals, Inc.

  11. Mother Vocal Recognition in Antarctic Fur Seal Arctocephalus gazella Pups: A Two-Step Process

    PubMed Central

    Jouventin, Pierre

    2015-01-01

    In otariids, mother’s recognition by pups is essential to their survival since females nurse exclusively their own young and can be very aggressive towards non-kin. Antarctic fur seal, Arctocephalus gazella, come ashore to breed and form dense colonies. During the 4-month lactation period, females alternate foraging trips at sea with suckling period ashore. On each return to the colony, females and pups first use vocalizations to find each other among several hundred conspecifics and olfaction is used as a final check. Such vocal identification has to be highly efficient. In this present study, we investigated the components of the individual vocal signature used by pups to identify their mothers by performing playback experiments on pups with synthetic signals. We thus tested the efficiency of this individual vocal signature by performing propagation tests and by testing pups at different playback distances. Pups use both amplitude and frequency modulations to identify their mother’s voice, as well as the energy spectrum. Propagation tests showed that frequency modulations propagated reliably up to 64m, whereas amplitude modulations and spectral content greatly were highly degraded for distances over 8m. Playback on pups at different distances suggested that the individual identification is a two-step process: at long range, pups identified first the frequency modulation pattern of their mother’s calls, and other components of the vocal signature at closer range. The individual vocal recognition system developed by Antarctic fur seals is well adapted to face the main constraint of finding kin in a crowd. PMID:26331475

  12. Coping with Heat: Function of The Natal Coat of Cape Fur Seal (Arctocephalus Pusillus Pusillus) Pups in Maintaining Core Body Temperature

    PubMed Central

    Erdsack, Nicola; Dehnhardt, Guido; Hanke, Wolf

    2013-01-01

    Cape fur seal (Arctocephalus pusillus) pups spend the first weeks of life exclusively or mainly ashore. They are exposed to intense solar radiation and high temperatures for long time periods, which results in temperatures up to at least 80°C on their black natal coat. To test the hypothesis that the natal coat has a crucial function in coping with these extreme conditions, we investigated the insulating properties of the natal coat in six captive newborn Cape fur seals during the first 50 days after birth. The natal fur differs from the adult fur not only in colour, but also in density, structure, and water repellence. We measured temperature on the fur surface and within the fur, as well as skin and rectal temperature under varying environmental conditions, comparable to the species' habitat. Experiments were designed to not influence the spontaneous behaviour of the pups. Rectal temperature was constant as long as the pups stayed dry, even during long-lasting intense solar radiation for up to 3 h. Skin temperature remained close to rectal temperature as long as the fur was dry, while with wet fur, skin temperature was significantly reduced as well. Our results show that the natal coat provides an effective insulation against overheating. The severely reduced insulation of wet natal fur against cold supports the assumption that the natal fur is an adaptation to the pups' terrestrial phase of life. PMID:23951287

  13. Coping with heat: function of the natal coat of cape fur seal (Arctocephalus Pusillus Pusillus) pups in maintaining core body temperature.

    PubMed

    Erdsack, Nicola; Dehnhardt, Guido; Hanke, Wolf

    2013-01-01

    Cape fur seal (Arctocephalus pusillus) pups spend the first weeks of life exclusively or mainly ashore. They are exposed to intense solar radiation and high temperatures for long time periods, which results in temperatures up to at least 80°C on their black natal coat. To test the hypothesis that the natal coat has a crucial function in coping with these extreme conditions, we investigated the insulating properties of the natal coat in six captive newborn Cape fur seals during the first 50 days after birth. The natal fur differs from the adult fur not only in colour, but also in density, structure, and water repellence. We measured temperature on the fur surface and within the fur, as well as skin and rectal temperature under varying environmental conditions, comparable to the species' habitat. Experiments were designed to not influence the spontaneous behaviour of the pups. Rectal temperature was constant as long as the pups stayed dry, even during long-lasting intense solar radiation for up to 3 h. Skin temperature remained close to rectal temperature as long as the fur was dry, while with wet fur, skin temperature was significantly reduced as well. Our results show that the natal coat provides an effective insulation against overheating. The severely reduced insulation of wet natal fur against cold supports the assumption that the natal fur is an adaptation to the pups' terrestrial phase of life.

  14. Foraging Behavior of Subantarctic Fur Seals Supports Efficiency of a Marine Reserve’s Design

    PubMed Central

    Kirkman, Stephen P.; Yemane, Dawit G.; Lamont, Tarron; Meÿer, Michael A.; Pistorius, Pierre A.

    2016-01-01

    Foraging behaviour of marine top predators is increasingly being used to identify areas of ecological importance. This is largely enabled by the ability of many such species to forage extensively in search of prey that is often concentrated in oceanographically productive areas. To identify important habitat in the Southern Indian Ocean within and around South Africa’s Prince Edward Islands’ Marine Protected Area (MPA), satellite transmitters were deployed on 12 lactating Subantarctic fur seals Arctocephalus tropicalis at Prince Edward Island (PEI) itself. Switching state space models were employed to correct ARGOS tracks and estimate behavioural states for locations along predicted tracks, namely travelling or area restricted search (ARS). A random forest model showed that distance from the study colony, longitude and distance from the Subantarctic Front were the most important predictors of suitable foraging habitat (inferred from ARS). Model-predicted suitable habitat occurred within the MPA in relatively close access to the colony during summer and autumn, but shifted northwards concurrently with frontal movements in winter and spring. The association of ARS with the MPA during summer-autumn was highly significant, highlighting the effectiveness of the recently declared reserve’s design for capturing suitable foraging habitat for this and probably other marine top predator species. PMID:27163373

  15. Gene discovery in the Antarctic fur seal (Arctocephalus gazella) skin transcriptome.

    PubMed

    Hoffman, Joseph I

    2011-07-01

    Next-generation sequencing provides a powerful new approach for developing functional genomic tools for nonmodel species, helping to narrow the gap between studies of model organisms and those of natural populations. Consequently, massively parallel 454 sequencing was used to characterize a normalized cDNA library derived from skin biopsy samples of twelve Antarctic fur seal (Arctocephalus gazella) individuals. Over 412 Mb of sequence data were generated, comprising 1.4 million reads of average length 286 bp. De novo assembly using Newbler 2.3 yielded 156 contigs plus 22 869 isotigs, which in turn clustered into 18,576 isogroups. Almost half of the assembled transcript sequences showed significant similarity to the nr database, revealing a functionally diverse array of genes. Moreover, 97.9% of these mapped to the dog (Canis lupis familiaris) genome, with a strong positive relationship between the number of sequences locating to a given chromosome and the length of that chromosome in the dog indicating a broad genomic distribution. Average depth of coverage was also almost 20-fold, sufficient to detect several thousand putative microsatellite loci and single nucleotide polymorphisms. This study constitutes an important step towards developing genomic resources with which to address consequential questions in pinniped ecology and evolution. It also supports an earlier but smaller study showing that skin tissue can be a rich source of expressed genes, with important implications for studying the genomics not only of marine mammals, but also more generally of species that cannot be destructively sampled.

  16. Resting metabolic rate and heat increment of feeding in juvenile South American fur seals (Arctocephalus australis).

    PubMed

    Dassis, M; Rodríguez, D H; Ieno, E N; Denuncio, P E; Loureiro, J; Davis, R W

    2014-02-01

    Bio-energetic models used to characterize an animal's energy budget require the accurate estimate of different variables such as the resting metabolic rate (RMR) and the heat increment of feeding (HIF). In this study, we estimated the in air RMR of wild juvenile South American fur seals (SAFS; Arctocephalus australis) temporarily held in captivity by measuring oxygen consumption while at rest in a postabsorptive condition. HIF, which is an increase in metabolic rate associated with digestion, assimilation and nutrient interconversion, was estimated as the difference in resting metabolic rate between the postabsorptive condition and the first 3.5h postprandial. As data were hierarchically structured, linear mixed effect models were used to compare RMR measures under both physiological conditions. Results indicated a significant increase (61%) for the postprandial RMR compared to the postabsorptive condition, estimated at 17.93±1.84 and 11.15±1.91mL O2 min(-1)kg(-1), respectively. These values constitute the first estimation of RMR and HIF in this species, and should be considered in the energy budgets for juvenile SAFS foraging at-sea.

  17. Phenotypic determinants of individual fitness in female fur seals: larger is better

    PubMed Central

    Beauplet, Gwénaël; Guinet, Christophe

    2007-01-01

    Inter-individual differences in fitness in female vertebrates have often been related to phenotypic discrepancies, suggesting that bigger individuals exhibit greater fitness. However, the use of the temporally variable indices of quality, such as body mass/condition, may not represent the most reliable index over longer time intervals. Few studies have assessed the direct influence of body size (BS) on individual fitness. We addressed this knowledge gap using data from long-term monitoring of individually marked female subantarctic fur seals. The females of higher quality (i.e. higher lifetime reproductive success) were larger in BS than their counterparts, which correlated with their ability to provision their pup with greater and more regular energy supply, possibly through the maximization of foraging performance and body fat storage. We accordingly found that our study population could be divided into three contrasted categories of maternal quality, with 33% of the females producing over 71% of the viable offspring constituting the next generation. We suggest that a larger BS represents a crucial selective advantage for a central place forager, especially when exploiting remotely available resources. PMID:17519194

  18. Flipper strokes can predict energy expenditure and locomotion costs in free-ranging northern and Antarctic fur seals

    PubMed Central

    Jeanniard-du-Dot, Tiphaine; Trites, Andrew W.; Arnould, John P. Y.; Speakman, John R.; Guinet, Christophe

    2016-01-01

    Flipper strokes have been proposed as proxies to estimate the energy expended by marine vertebrates while foraging at sea, but this has never been validated on free-ranging otariids (fur seals and sea lions). Our goal was to investigate how well flipper strokes correlate with energy expenditure in 33 foraging northern and Antarctic fur seals equipped with accelerometers, GPS, and time-depth recorders. We concomitantly measured field metabolic rates with the doubly-labelled water method and derived activity-specific energy expenditures using fine-scale time-activity budgets for each seal. Flipper strokes were detected while diving or surface transiting using dynamic acceleration. Despite some inter-species differences in flipper stroke dynamics or frequencies, both species of fur seals spent 3.79 ± 0.39 J/kg per stroke and had a cost of transport of ~1.6–1.9 J/kg/m while diving. Also, flipper stroke counts were good predictors of energy spent while diving (R2 = 0.76) and to a lesser extent while transiting (R2 = 0.63). However, flipper stroke count was a poor predictor overall of total energy spent during a full foraging trip (R2 = 0.50). Amplitude of flipper strokes (i.e., acceleration amplitude × number of strokes) predicted total energy expenditure (R2 = 0.63) better than flipper stroke counts, but was not as accurate as other acceleration-based proxies, i.e. Overall Dynamic Body Acceleration. PMID:27658718

  19. Temporal Allocation of Foraging Effort in Female Australian Fur Seals (Arctocephalus pusillus doriferus)

    PubMed Central

    Hoskins, Andrew J.; Arnould, John P. Y.

    2013-01-01

    Across an individual's life, foraging decisions will be affected by multiple intrinsic and extrinsic drivers that act at differing timescales. This study aimed to assess how female Australian fur seals allocated foraging effort and the behavioural changes used to achieve this at three temporal scales: within a day, across a foraging trip and across the final six months of the lactation period. Foraging effort peaked during daylight hours (57% of time diving) with lulls in activity just prior to and after daylight. Dive duration reduced across the day (196 s to 168 s) but this was compensated for by an increase in the vertical travel rate (1500–1600 m·h−1) and a reduction in postdive duration (111–90 s). This suggests physiological constraints (digestive costs) or prey availability may be limiting mean dive durations as a day progresses. During short trips (<2.9 d), effort remained steady at 55% of time diving, whereas, on long trips (>2.9 d) effort increased up to 2–3 d and then decreased. Dive duration decreased at the same rate in short and long trips, respectively, before stabilising (long trips) between 4–5 d. Suggesting that the same processes (digestive costs or prey availability) working at the daily scale may also be present across a trip. Across the lactation period, foraging effort, dive duration and vertical travel rate increased until August, before beginning to decrease. This suggests that as the nutritional demands of the suckling pup and developing foetus increase, female effort increases to accommodate this, providing insight into the potential constraints of maternal investment in this species. PMID:24244511

  20. Hematology, Serum Chemistry, and Early Hematologic Changes in Free-Ranging South American Fur Seals ( Arctocephalus australis ) at Guafo Island, Chilean Patagonia.

    PubMed

    Seguel, Mauricio; Muñoz, Francisco; Keenan, Alessandra; Perez-Venegas, Diego J; DeRango, Eugene; Paves, Hector; Gottdenker, Nicole; Müller, Ananda

    2016-07-01

    The establishment of clinical pathology baseline data is critical to evaluate temporal and spatial changes in marine mammal groups. Despite increased availability of studies on hematology and biochemistry of marine mammals, reference ranges are lacking for many populations, especially among fur seal species. During the austral summers of 2014 and 2015, we evaluated basic hematologic and biochemical parameters in clinically healthy, physically restrained South American fur seal ( Arctocephalus australis ) lactating females and 2-mo-old pups. We also assessed the temporal variation of hematology parameters on the pups during their first 2 mo of life. Reference ranges of lactating females were similar to those previously reported in other fur seal species. In the case of pups, reference ranges are similar to values previously reported in sea lion species. As expected, most biochemical and hematologic values differ significantly between adult females and pups. As in other otariids, South American fur seals pups are born with higher values of total red blood cells, hemoglobin, and packed cell volume, and lower numbers of total leukocytes, neutrophils, lymphocytes, and eosinophils. To the best of our knowledge, data on hematology reference values for South American fur seals has not been previously reported and is useful for continued health monitoring of this species, as well as for comparisons with other otariid groups.

  1. SURVEY FOR INFECTIOUS DISEASE IN THE SOUTH AMERICAN FUR SEAL (ARCTOCEPHALUS AUSTRALIS) POPULATION AT PUNTA SAN JUAN, PERU.

    PubMed

    Jankowski, Gwen; Adkesson, Michael J; Saliki, Jeremiah T; Cárdenas-Alayza, Susana; Majluf, Patricia

    2015-06-01

    The Peruvian population of the South American fur seal ( Arctocephalus australis ) is a distinct evolutionarily significant unit that is endangered. One of the largest rookeries for this species in Peru is located within the Punta San Juan marine protected area (15°22'S, 75°12'W). To better understand the current health status of this population, exposure to 10 pinniped pathogens was evaluated in adult female fur seals (n=29) via serology and polymerase chain reaction (PCR) techniques in November 2010. The results suggest this population is naïve to canine and phocine distemper viruses (serum neutralization test), five Leptospira interrogans serovars (microscopic agglutination test), and Brucella canis (card test). Indirect fluorescent antibody testing for Toxoplasma gondii , Neospora caninum , and Sarcocystis neurona was also uniformly negative. PCR testing of nasal swabs using previously described Mycoplasma spp. primers was positive in 37.9% (11/29) of samples. One animal was positive via card test for Brucella abortus , whereas 53.7% (15/28) were positive or suspect using a marine Brucella competitive enzyme-linked immunosorbent assay. Antibody to phocine herpesvirus-1 (PHV-1) was identified in 85.7% (24/28) of the sampled population by serum neutralization testing. Overall, exposure to Mycoplasma spp., Brucella spp., and PHV-1 was observed, but results demonstrated low to no exposure to many key pinniped pathogens. The expansion of human populations, agriculture, and industry along the Peruvian coast may lead to increased pathogen exposure from human, domestic, and wild animal sources. The naïve nature of this key population of South American fur seals raises concerns about potential risk for disease outbreaks.

  2. Same size--same niche? Foraging niche separation between sympatric juvenile Galapagos sea lions and adult Galapagos fur seals.

    PubMed

    Jeglinski, Jana W E; Goetz, Kimberley T; Werner, Christiane; Costa, Daniel P; Trillmich, Fritz

    2013-05-01

    1. In vertebrates, patterns of resource utilization change throughout development according to age- and or size-specific abilities and requirements. Thus, interspecific competition affects different age classes differently. 2. Adults of sympatric species often show distinct foraging niche segregation, but juvenile resource use might overlap with adult competitors of similar body size. Resultant negative effects on juveniles can have important consequences for population dynamics, yet such interactions have received little attention in studies of mammalian communities. 3. Using GPS tracking devices, time-depth recorders and stable isotope data, we compared diving depth, activity time, trophic position and foraging habitat characteristics to investigate foraging niche overlap between similar-sized sympatric juvenile Galapagos sea lions (Zalophus wollebaeki) and adult Galapagos fur seals (Arctocephalus galapagoensis) and compared each group with much larger-bodied adult Galapagos sea lions. 4. We found little indication for direct competition but a complex pattern of foraging niche segregation: juvenile sea lions and adult fur seals dived to shallow depths at night, but foraged in different habitats with limited spatial overlap. Conversely, juvenile and adult sea lions employed different foraging patterns, but their foraging areas overlapped almost completely. 5. Consistency of foraging habitat characteristics between juvenile and adult sea lions suggests that avoidance of competition may be important in shaping foraging habitat utilization. Resultant specialization on a limited habitat could contribute to low sea lion numbers that contrast with high fur seal abundance. Our data suggest that exploitation by multiple predators within spatially restricted foraging ranges of juveniles might negatively impact juvenile foraging success and ultimately influence population dynamics.

  3. Australian Fur Seals (Arctocephalus pusillus doriferus) Use Raptorial Biting and Suction Feeding When Targeting Prey in Different Foraging Scenarios

    PubMed Central

    Hocking, David P.; Salverson, Marcia; Fitzgerald, Erich M. G.; Evans, Alistair R.

    2014-01-01

    Foraging behaviours used by two female Australian fur seals (Arctocephalus pusillus doriferus) were documented during controlled feeding trials. During these trials the seals were presented with prey either free-floating in open water or concealed within a mobile ball or a static box feeding device. When targeting free-floating prey both subjects primarily used raptorial biting in combination with suction, which was used to draw prey to within range of the teeth. When targeting prey concealed within either the mobile or static feeding device, the seals were able to use suction to draw out prey items that could not be reached by biting. Suction was followed by lateral water expulsion, where water drawn into the mouth along with the prey item was purged via the sides of the mouth. Vibrissae were used to explore the surface of the feeding devices, especially when locating the openings in which the prey items had been hidden. The mobile ball device was also manipulated by pushing it with the muzzle to knock out concealed prey, which was not possible when using the static feeding device. To knock prey out of this static device one seal used targeted bubble blowing, where a focused stream of bubbles was blown out of the nose into the openings in the device. Once captured in the jaws, prey items were manipulated and re-oriented using further mouth movements or chews so that they could be swallowed head first. While most items were swallowed whole underwater, some were instead taken to the surface and held in the teeth, while being vigorously shaken to break them into smaller pieces before swallowing. The behavioural flexibility displayed by Australian fur seals likely assists in capturing and consuming the extremely wide range of prey types that are targeted in the wild, during both benthic and epipelagic foraging. PMID:25390347

  4. Intestinal helminth fauna of the South American sea lion Otaria flavescens and fur seal Arctocephalus australis from northern Patagonia, Argentina.

    PubMed

    Hernández-Orts, J S; Montero, F E; Juan-García, A; García, N A; Crespo, E A; Raga, J A; Aznar, F J

    2013-09-01

    We report on the intestinal helminth fauna of 56 South American sea lions, Otaria flavescens, and 5 South American fur seals, Arctocephalus australis, from northern Patagonia, Argentina. A total of 97,325 helminth specimens were collected from sea lions. Gravid individuals were represented by 6 species of parasites: 1 digenean (Ascocotyle (Ascocotyle) patagoniensis), 1 cestode (Diphyllobothrium spp.), 3 nematodes (Uncinaria hamiltoni, Contracaecum ogmorhini s.s., Pseudoterranova cattani) and 1 acanthocephalan (Corynosoma australe). In addition, third-stage larvae of 2 nematodes (Contracaecum sp. and Anisakis sp. type I) and 3 juvenile acanthocephalans (Andracantha sp., Profilicollis chasmagnathi and Corynosoma cetaceum) were also collected. Andracantha sp., C. ogmorhini s.s. and P. chasmagnathi represent new host records. A total of 1516 helminth specimens were collected from fur seals. Gravid individuals were represented by three species of parasites, namely, Diphyllobothrium spp., C. ogmorhini s.s. and C. australe. In addition, larvae of Contracaecum sp. and P. cattani, juveniles of C. cetaceum and immature cestodes (Tetrabothriidae gen. sp.) were also collected. Corynosoma australe was the most prevalent and abundant parasite in both hosts, accounting for >90% of all specimens. Sea lions and furs seals from northern Patagonia harbour the intestinal helminth communities that could be predicted for otariids, i.e. the combination of species of the genera Corynosoma, Diphyllobothrium, Pseudoterranova, Contracaecum and, in pups, Uncinaria. Additionally, both species of otariid are apparently unsuitable hosts (i.e. non-hosts) for as many as five parasite taxa. The inclusion or exclusion of these species affects estimation of species richness at both component community (11 versus 6 species in sea lions; 7 versus 3 species in fur seals) and infracommunity (mean: 3.1 versus 2.6 in sea lions; 2.2 versus 1.7 species) levels. Information about the reproductive status of

  5. Unique fur and skin structure in harbour seals (Phoca vitulina)—thermal insulation, drag reduction, or both?

    PubMed Central

    Erdsack, Nicola; Dehnhardt, Guido; Witt, Martin; Wree, Andreas; Siebert, Ursula; Hanke, Wolf

    2015-01-01

    Vertebrate surface structures, including mammalian skin and hair structures, have undergone various modifications during evolution in accordance with functional specializations. Harbour seals rely on their vibrissal system for orientation and foraging. To maintain tactile sensitivity even at low temperatures, the vibrissal follicles are heated up intensely, which could cause severe heat loss to the environment. We analysed skin samples of different body parts of harbour seals, and expected to see higher hair densities at the vibrissal pads as a way to reduce heat loss. In addition to significantly higher hair densities around the vibrissae than on the rest of the body, we show a unique fur structure of hair bundles consisting of broad guard hairs along with hairs of a new type, smaller than guard hairs but broader than underhairs, which we defined as ‘intermediate hairs’. This fur composition has not been reported for any mammal so far and may serve for thermal insulation as well as drag reduction. Furthermore, we describe a scale-like skin structure that also presumably plays a role in drag reduction. PMID:25652462

  6. Unique fur and skin structure in harbour seals (Phoca vitulina)--thermal insulation, drag reduction, or both?

    PubMed

    Erdsack, Nicola; Dehnhardt, Guido; Witt, Martin; Wree, Andreas; Siebert, Ursula; Hanke, Wolf

    2015-03-06

    Vertebrate surface structures, including mammalian skin and hair structures, have undergone various modifications during evolution in accordance with functional specializations. Harbour seals rely on their vibrissal system for orientation and foraging. To maintain tactile sensitivity even at low temperatures, the vibrissal follicles are heated up intensely, which could cause severe heat loss to the environment. We analysed skin samples of different body parts of harbour seals, and expected to see higher hair densities at the vibrissal pads as a way to reduce heat loss. In addition to significantly higher hair densities around the vibrissae than on the rest of the body, we show a unique fur structure of hair bundles consisting of broad guard hairs along with hairs of a new type, smaller than guard hairs but broader than underhairs, which we defined as 'intermediate hairs'. This fur composition has not been reported for any mammal so far and may serve for thermal insulation as well as drag reduction. Furthermore, we describe a scale-like skin structure that also presumably plays a role in drag reduction.

  7. Final Environmental Assessment for Minuteman III Modification

    DTIC Science & Technology

    2007-11-02

    lion ( Zalophus californianus ), Pacific harbor seal (Phoca vitulina), northern elephant seal (Mirounga angustirostris), and northern fur seal...Northern fur seal Callorhinus ursinus MMPA May – Nov X Guadalupe fur seal Arctocephalus townsendi T, St - X X X California sea lion Zalophus californianus ...sites & nearshore waters) Guadalupe fur seal Arctocephalus townsendi T T Northern fur seal Callorhinus ursinus MMPA - California sea lion Zalophus

  8. Tissue Distribution of Polychlorinated Biphenyls and Organochlorine Pesticides and Potential Toxicity to Alaskan Northern Fur Seals Assessed Using PCBs Congener Specific Mode of Action Schemes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The concentrations of 145 polychlorinated biphenyl (PCB) congeners were measured using gas chromatography-ion trap mass spectrometry in 8 different tissues (blubber, brain, heart, kidney, liver, lung, muscle, and reproductive tissues) of 10 Alaskan northern fur seals. The mean concentrations of bot...

  9. Top-down and bottom-up influences on demographic rates of Antarctic fur seals Arctocephalus gazella.

    PubMed

    Schwarz, Lisa K; Goebel, Michael E; Costa, Daniel P; Kilpatrick, A Marm

    2013-07-01

    Two major drivers in population dynamics are bottom-up processes, such as environmental factors that affect foraging success, and the top-down impacts of predation. Many populations of marine mammal and seabird species appear to be declining in response to reductions in prey associated with the bottom-up effects of climate change. However, predation, which usually occurs at sea and is difficult to observe, may also play a key role. We analysed drivers of population dynamics of Antarctic fur seals Arctocephalus gazella at Cape Shirreff from 1997 to 2009, including a predator that targets pre-weaned pups and bottom-up environmental effects in an ecosystem particularly sensitive to small changes in temperature. We use Bayesian mark-recapture analysis to demonstrate that although large-scale environmental variability affects annual adult survival and reproduction, first year survival appears to be driving the current decline in this population (as defined by a decline in the annual number of pups born). Although the number of pups increased during the first third of the study, first year survival and recruitment of those pups in later years was very low. Such low survival may be driven by leopard seal Hydrurga leptonyx predation, particularly prior to weaning. Our results suggest that without leopard seal predation, this population would most likely increase in size, despite the observed bottom-up effects of climate changes on adult vital rates. More broadly, our results show how age-targeted predation could be a major factor in population decline of K-selected colonial breeders.

  10. Two behavioural traits promote fine-scale species segregation and moderate hybridisation in a recovering sympatric fur seal population

    PubMed Central

    2010-01-01

    Background In systems where two or more species experience secondary contact, behavioural factors that regulate interspecific gene flow may be important for maintaining species boundaries and reducing the incidence of hybridisation. At subantarctic Macquarie Island, two species of fur seal breed in close proximity to one another, hybridise at very high levels (up to 21% of hybrid pups are born annually), yet retain discrete gene pools. Using spatial and genetic information collected for pups and adults over twelve years, we assessed two behavioural traits - inter-annual site fidelity and differences in habitat use between the species - as possible contributors to the maintenance of this species segregation. Further, we explored the breakdown of these traits in pure-species individuals and hybrids. Results We found virtually complete spatial segregation of the parental species, with only one exception; a single territory that contained adults of both species and also the highest concentration of hybrid pups. The spatial distribution of each species was closely linked to habitat type (pebbled vs boulder beaches), with members of each species breeding almost exclusively on one type or the other but hybrids breeding on both or at the junction between habitats. Inter-annual site fidelity was high for both sexes of pure-species adults, with 66% of females and all males returning to the same territory or a neighbouring one in different years. An important consequence for pure females of breeding on the 'wrong' habitat type, and thus in a heterospecific aggregation, was the production of hybrid pups. Low habitat fidelity of hybrid females facilitated bi-directional backcrossing, resulting in more diverse hybrid offspring. Conclusion In a disturbed system where two sympatric fur seal species breed in close proximity, discrete gene pools are retained by extremely fine-scale and strong spatial segregation of the species. Two behavioural traits were found to be important in

  11. Tuberculosis in sea lions and fur seals from the south-western Atlantic coast.

    PubMed

    Bernardelli, A; Bastida, R; Loureiro, J; Michelis, H; Romano, M I; Cataldi, A; Costa, E

    1996-09-01

    Diverse pathological conditions causing the strandings and/or deaths of several species of sea lions and seals on the northern coast of the province of Buenos Aires are being studied. Tuberculosis was diagnosed in six cases of strandings, involving two otariid seal species (one Otaria flavescens and five Arctocephalus australis), between March 1989 and December 1992. Necropsies were performed on all six cases. Granulomatous lesions were observed in the prescapular and hepatic lymph nodes. Lesions were also seen in the lungs, pleura, liver, spleen and peritoneum. Bacteriological isolation was attempted from all the samples. The isolates were identified as belonging to the Mycobacterium tuberculosis complex. Some showed characteristics consistent with M. bovis, whereas others demonstrated properties of M. tuberculosis. Genomic deoxyribonucleic acid (DNA) from these strains was analysed by restriction fragment length polymorphism (RFLP), using IS6110, a genetic marker found only in the Mycobacterium tuberculosis complex. Using the IS6110 probe, similar fingerprints were obtained, suggesting a common source of infection. However, the pattern of DNA differed from DNA patterns of M. bovis isolated from humans and cattle in Argentina, which generally contain a unique 1.9 kbp band. These results suggest that mycobacteria isolated from wild seals form a different grouping inside the M. tuberculosis complex. This is the first time that tuberculosis has been detected in wild seals from the south-western Atlantic coast.

  12. Morphology of the lingual surface of South American fur seal (Arctocephalus australis) and sea lion (Otaria flavescens).

    PubMed

    Erdoğan, Serkan; Villar Arias, Silvia; Pérez, William

    2015-02-01

    In this study, we aimed to describe the morphological characteristics of the lingual papillae in two species of Otariidae family by stereomicroscopy and scanning electron microscopy. We used tongues of two South American Otariidae species. The tongues were elongated and terminated in bifid apex and there was no median sulcus on the dorsal lingual surface. The most numerous type of lingual papilla was filiform in the South American fur seal (SASL) and entire dorsal lingual surface was covered by these filiform papillae but the dorsal surface of the tongue of the South American sea lion was covered by numerous polygonal projections, which were different in size. Fungiform papillae were detected in only SASL and they randomly distributed on the lingual apex and body, and some fungiform papillae were collected into twosome or threesome groups on the posterior part of the lingual body. Circumvallate papilla was found in the center of the lingual radix of South American sea lion. Thread-like conical papillae were common for both species and they located on the lingual radix. We determined that lingual surface morphology was completely different in each species, although they were members of the same family, Otariidae.

  13. The first report of otarine herpesvirus-1-associated urogenital carcinoma in a South American fur seal (Arctocephalus australis).

    PubMed

    Dagleish, M P; Barrows, M; Maley, M; Killick, R; Finlayson, J; Goodchild, R; Valentine, A; Saunders, R; Willoughby, K; Smith, K C; Stidworthy, M F

    2013-07-01

    Otarine herpesvirus (OtHV)-1-associated urogenital carcinoma has been well documented in the California sea lion (Zalophus californianus, CSL), but this is the first report of this tumour in a captive South American fur seal (Arctocephalus australis, SAFS). The gross and microscopical morphology of the tumour in the SAFS was identical to that described previously in CSLs and the tumour in the present case had metastasized within the urogenital tract and draining lymph nodes and to the lungs and one kidney. Immunohistochemistry revealed intra- and extracytoplasmic labelling of herpesvirus antigen in the cells of the tumour tissue and transitional epithelium of the urethra. OtHV-1 nucleic acids were detected within tumour tissue and from a urogenital swab by polymerase chain reaction. The ranges of these two species of pinniped do not overlap normally in the wild, suggesting that transmission of OtHV-1 probably occurred in captivity. This confirmed susceptibility of the SAFS to the development of OtHV-1-associated urogenital carcinoma suggests that all species of Otariidae should be screened for OtHV-1 infection prior to movement within and between zoological collections.

  14. Lunar cycles in diel prey migrations exert a stronger effect on the diving of juveniles than adult Galápagos fur seals.

    PubMed

    Horning, M; Trillmich, F

    1999-06-07

    In our study of the development of diving in Galápagos fur seals, we analysed changes in diving activity and body mass trends over the lunar cycle. Based on previously observed lunar cycles in colony attendance patterns, we hypothesized a greater impact of prey migrations of deep scattering layer organisms on younger fur seals. Using electronic dive recorders, we determined that seals dived less and deeper on moonlit nights than at new moon, and incurred body mass losses. These changes in foraging over the lunar cycle correlate with the suppression of the vertical migration of prey by lunar light. All effects were more pronounced in juveniles than adult females, with greater relative mass loss during full moon, which must (i) negatively affect long-term juvenile growth rates, (ii) lengthen periods of maternal dependence, and (iii) contribute to the lowest reproductive rate reported for seals. This underlines the importance of studying ontogeny in order to understand life histories, and for determining the susceptibility of animal populations to fluctuations in food availability.

  15. Use of Anthropogenic Sea Floor Structures by Australian Fur Seals: Potential Positive Ecological Impacts of Marine Industrial Development?

    PubMed Central

    Arnould, John P. Y.; Monk, Jacquomo; Ierodiaconou, Daniel; Hindell, Mark A.; Semmens, Jayson; Hoskins, Andrew J.; Costa, Daniel P.; Abernathy, Kyler; Marshall, Greg J.

    2015-01-01

    Human-induced changes to habitats can have deleterious effects on many species that occupy them. However, some species can adapt and even benefit from such modifications. Artificial reefs have long been used to provide habitat for invertebrate communities and promote local fish populations. With the increasing demand for energy resources within ocean systems, there has been an expansion of infrastructure in near-shore benthic environments which function as de facto artificial reefs. Little is known of their use by marine mammals. In this study, the influence of anthropogenic sea floor structures (pipelines, cable routes, wells and shipwrecks) on the foraging locations of 36 adult female Australian fur seals (Arctocephalus pusillus doriferus) was investigated. For 9 (25%) of the individuals, distance to anthropogenic sea floor structures was the most important factor in determining the location of intensive foraging activity. Whereas the influence of anthropogenic sea floor structures on foraging locations was not related to age and mass, it was positively related to flipper length/standard length (a factor which can affect manoeuvrability). A total of 26 (72%) individuals tracked with GPS were recorded spending time in the vicinity of structures (from <1% to >75% of the foraging trip duration) with pipelines and cable routes being the most frequented. No relationships were found between the amount of time spent frequenting anthropogenic structures and individual characteristics. More than a third (35%) of animals foraging near anthropogenic sea floor structures visited more than one type of structure. These results further highlight potentially beneficial ecological outcomes of marine industrial development. PMID:26132329

  16. Return Customers: Foraging Site Fidelity and the Effect of Environmental Variability in Wide-Ranging Antarctic Fur Seals

    PubMed Central

    Arthur, Benjamin; Hindell, Mark; Bester, Marthan; Trathan, Phil; Jonsen, Ian; Staniland, Iain; Oosthuizen, W. Chris; Wege, Mia; Lea, Mary-Anne

    2015-01-01

    Strategies employed by wide-ranging foraging animals involve consideration of habitat quality and predictability and should maximise net energy gain. Fidelity to foraging sites is common in areas of high resource availability or where predictable changes in resource availability occur. However, if resource availability is heterogeneous or unpredictable, as it often is in marine environments, then habitat familiarity may also present ecological benefits to individuals. We examined the winter foraging distribution of female Antarctic fur seals, Arctocephalus gazelle, over four years to assess the degree of foraging site fidelity at two scales; within and between years. On average, between-year fidelity was strong, with most individuals utilising more than half of their annual foraging home range over multiple years. However, fidelity was a bimodal strategy among individuals, with five out of eight animals recording between-year overlap values of greater than 50%, while three animals recorded values of less than 5%. High long-term variance in sea surface temperature, a potential proxy for elevated long-term productivity and prey availability, typified areas of overlap. Within-year foraging site fidelity was weak, indicating that successive trips over the winter target different geographic areas. We suggest that over a season, changes in prey availability are predictable enough for individuals to shift foraging area in response, with limited associated energetic costs. Conversely, over multiple years, the availability of prey resources is less spatially and temporally predictable, increasing the potential costs of shifting foraging area and favouring long-term site fidelity. In a dynamic and patchy environment, multi-year foraging site fidelity may confer a long-term energetic advantage to the individual. Such behaviours that operate at the individual level have evolutionary and ecological implications and are potential drivers of niche specialization and modifiers of

  17. Dive characteristics can predict foraging success in Australian fur seals (Arctocephalus pusillus doriferus) as validated by animal-borne video

    PubMed Central

    Volpov, Beth L.; Rosen, David A. S.; Hoskins, Andrew J.; Lourie, Holly J.; Dorville, Nicole; Baylis, Alastair M. M.; Wheatley, Kathryn E.; Marshall, Greg; Abernathy, Kyler; Semmens, Jayson; Hindell, Mark A.; Arnould, John P. Y.

    2016-01-01

    ABSTRACT Dive characteristics and dive shape are often used to infer foraging success in pinnipeds. However, these inferences have not been directly validated in the field with video, and it remains unclear if this method can be applied to benthic foraging animals. This study assessed the ability of dive characteristics from time-depth recorders (TDR) to predict attempted prey capture events (APC) that were directly observed on animal-borne video in Australian fur seals (Arctocephalus pusillus doriferus, n=11). The most parsimonious model predicting the probability of a dive with ≥1 APC on video included only descent rate as a predictor variable. The majority (94%) of the 389 total APC were successful, and the majority of the dives (68%) contained at least one successful APC. The best model predicting these successful dives included descent rate as a predictor. Comparisons of the TDR model predictions to video yielded a maximum accuracy of 77.5% in classifying dives as either APC or non-APC or 77.1% in classifying dives as successful verses unsuccessful. Foraging intensity, measured as either total APC per dive or total successful APC per dive, was best predicted by bottom duration and ascent rate. The accuracy in predicting total APC per dive varied based on the number of APC per dive with maximum accuracy occurring at 1 APC for both total (54%) and only successful APC (52%). Results from this study linking verified foraging dives to dive characteristics potentially opens the door to decades of historical TDR datasets across several otariid species. PMID:26873950

  18. Use of Anthropogenic Sea Floor Structures by Australian Fur Seals: Potential Positive Ecological Impacts of Marine Industrial Development?

    PubMed

    Arnould, John P Y; Monk, Jacquomo; Ierodiaconou, Daniel; Hindell, Mark A; Semmens, Jayson; Hoskins, Andrew J; Costa, Daniel P; Abernathy, Kyler; Marshall, Greg J

    2015-01-01

    Human-induced changes to habitats can have deleterious effects on many species that occupy them. However, some species can adapt and even benefit from such modifications. Artificial reefs have long been used to provide habitat for invertebrate communities and promote local fish populations. With the increasing demand for energy resources within ocean systems, there has been an expansion of infrastructure in near-shore benthic environments which function as de facto artificial reefs. Little is known of their use by marine mammals. In this study, the influence of anthropogenic sea floor structures (pipelines, cable routes, wells and shipwrecks) on the foraging locations of 36 adult female Australian fur seals (Arctocephalus pusillus doriferus) was investigated. For 9 (25%) of the individuals, distance to anthropogenic sea floor structures was the most important factor in determining the location of intensive foraging activity. Whereas the influence of anthropogenic sea floor structures on foraging locations was not related to age and mass, it was positively related to flipper length/standard length (a factor which can affect manoeuvrability). A total of 26 (72%) individuals tracked with GPS were recorded spending time in the vicinity of structures (from <1% to >75% of the foraging trip duration) with pipelines and cable routes being the most frequented. No relationships were found between the amount of time spent frequenting anthropogenic structures and individual characteristics. More than a third (35%) of animals foraging near anthropogenic sea floor structures visited more than one type of structure. These results further highlight potentially beneficial ecological outcomes of marine industrial development.

  19. Return customers: foraging site fidelity and the effect of environmental variability in wide-ranging antarctic fur seals.

    PubMed

    Arthur, Benjamin; Hindell, Mark; Bester, Marthan; Trathan, Phil; Jonsen, Ian; Staniland, Iain; Oosthuizen, W Chris; Wege, Mia; Lea, Mary-Anne

    2015-01-01

    Strategies employed by wide-ranging foraging animals involve consideration of habitat quality and predictability and should maximise net energy gain. Fidelity to foraging sites is common in areas of high resource availability or where predictable changes in resource availability occur. However, if resource availability is heterogeneous or unpredictable, as it often is in marine environments, then habitat familiarity may also present ecological benefits to individuals. We examined the winter foraging distribution of female Antarctic fur seals, Arctocephalus gazelle, over four years to assess the degree of foraging site fidelity at two scales; within and between years. On average, between-year fidelity was strong, with most individuals utilising more than half of their annual foraging home range over multiple years. However, fidelity was a bimodal strategy among individuals, with five out of eight animals recording between-year overlap values of greater than 50%, while three animals recorded values of less than 5%. High long-term variance in sea surface temperature, a potential proxy for elevated long-term productivity and prey availability, typified areas of overlap. Within-year foraging site fidelity was weak, indicating that successive trips over the winter target different geographic areas. We suggest that over a season, changes in prey availability are predictable enough for individuals to shift foraging area in response, with limited associated energetic costs. Conversely, over multiple years, the availability of prey resources is less spatially and temporally predictable, increasing the potential costs of shifting foraging area and favouring long-term site fidelity. In a dynamic and patchy environment, multi-year foraging site fidelity may confer a long-term energetic advantage to the individual. Such behaviours that operate at the individual level have evolutionary and ecological implications and are potential drivers of niche specialization and modifiers of

  20. 75 FR 54094 - Marine Mammals; File No. 486-1790

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-03

    ... capture of California sea lions (Zalophus californianus), northern elephant seals (Mirounga angustirostris), harbor seals (Phoca vitulina), and northern fur seals (Callorhinus ursinus) of any age on the...

  1. 76 FR 30309 - Marine Mammals; File No. 16087

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ... sea lions (Zalophus californianus), Pacific harbor seals (Phoca vitulina), and northern elephant seals (Mirounga angustrirostris), with incidental harassment of northern fur seals (Callorhinus ursinus)....

  2. Variation in the fatty acid composition of blubber in Cape fur seals (Arctocephalus pusillus pusillus) and the implications for dietary interpretation.

    PubMed

    Arnould, J P Y; Nelson, M M; Nichols, P D; Oosthuizen, W H

    2005-05-01

    Analysis of the fatty acid (FA) composition of blubber is a valuable tool in interpreting the diet of marine mammals. This technique is based on the principle that particular FA present in prey can be incorporated largely untransformed into predator adipose tissue stores, thereby providing biochemical signatures with which to identify prey species. Several studies of phocid seals and cetaceans have documented vertical stratification in the FA composition of blubber such that inferences about diet may vary greatly depending on the layer of the blubber that is analysed. It is not known whether blubber in otariid seals (fur seals and sea lions) also displays vertical stratification in FA composition. Furthermore, it is not known whether the FA composition of blubber is uniform in these species. In the present study, the vertical and regional variation in FA composition of blubber was investigated in seven adult female Cape fur seals (Arctocephalus pusillus pusillus). The proportion of monounsaturated fatty acids (MUFA) was greater in the outer (43.6+/-1.3%) than inner portion (40.9+/-1.2%; t(20)=5.59, P<0.001) whereas the proportions were greater in the inner than outer portions for saturated fatty acids (23.6+/-0.5% and 21.9+/-0.6%, respectively, t(20) = 5.31, P<0.001) and polyunsaturated fatty acids (PUFA, 35.5+/-0.7% and 34.5+/-0.7%, respectively, t(20) = 3.81, P < 0.001). There was an inverse relationship between MUFA and PUFA in the blubber, independent of sampling location. In addition, with the exception of the inner portion from non-lactating females, blubber from the mammary area had the highest proportions of 18:1omega9c and total MUFA, followed by blubber from the rump and neck, suggesting that the deposition and mobilisation of blubber lipids may not be uniform around the body in otariid seals. These results support the need for blubber tissue to be sampled from the same site on animals, and to the full depth of the blubber layer, to minimise variation in

  3. Organochlorine contaminants in blubber of four seal species: integrating biomonitoring and specimen banking.

    PubMed

    Krahn, M M; Becker, P R; Tilbury, K L; Stein, J E

    1997-05-01

    Blubber samples from four Alaska seal species (bearded seal, Erignathus barbatus, harbor seal, Phoca vitulina, northern fur seal, Callorhinus ursinus, ringed seal, P. hispida) were collected for inclusion in the US National Biomonitoring Specimen Bank, as well as for immediate analysis as part of the contaminant monitoring component of the US National Marine Fisheries Service's Marine Mammal Health and Stranding Response Program. The blubber samples were analyzed for organochlorine (OC) contaminants (e.g., PCB congeners, pesticides, DDTs). Results for bearded and ringed seals from the Alaska Arctic revealed low blubber concentrations of OC contaminants. Harbor seals from Prince William Sound. Gulf of Alaska, had somewhat higher blubber concentrations of OC contaminants. In contrast, northern fur seals sampled from the Pribilof Islands had blubber concentrations of certain OC contaminants that were about an order of magnitude higher than those found in the other seal species. Differences in contaminant concentrations among the Alaska seals may be explained by differences in feeding habits and migratory patterns, age or gender did not appear to account for the differences observed. The highest concentrations of OCs were found in harbor seals stranded along the northwestern US mainland, which is consistent with higher concentrations of anthropogenic contaminants being found in urban coastal areas than in more remote Arctic environments. The integration of real-time contaminant monitoring with specimen banking provides important baseline data that can be used to plan and manage banking activities. This includes identifying appropriate specimens that are useful in assessing temporal trends and increasing the utility of the banked samples in assessing chemical contaminant accumulation and relationships to biological effects.

  4. Entanglement of Australian sea lions and New Zealand fur seals in lost fishing gear and other marine debris before and after Government and industry attempts to reduce the problem.

    PubMed

    Page, Brad; McKenzie, Jane; McIntosh, Rebecca; Baylis, Alastair; Morrissey, Adam; Calvert, Norna; Haase, Tami; Berris, Mel; Dowie, Dave; Shaughnessy, Peter D; Goldsworthy, Simon D

    2004-07-01

    In recent years, Australian governments and fishing industry associations have developed guiding principles aimed at reducing the impact of fishing on non-target species and the benthos and increasing community awareness of their efforts. To determine whether they reduced seal entanglement in lost fishing gear and other marine debris, we analysed Australian sea lion and New Zealand fur seal entanglement data collected from Kangaroo Island, South Australia. Contrary to our expectations, we found that entanglement rates did not decrease in recent years. The Australian sea lion entanglement rate (1.3% in 2002) and the New Zealand fur seal entanglement rate (0.9% in 2002) are the third and fourth highest reported for any seal species. Australian sea lions were most frequently entangled in monofilament gillnet that most likely originated from the shark fishery, which operates in the region where sea lions forage--south and east of Kangaroo Island. In contrast, New Zealand fur seals were most commonly entangled in loops of packing tape and trawl net fragments suspected to be from regional rock lobster and trawl fisheries. Based on recent entanglement studies, we estimate that 1478 seals die from entanglement each year in Australia. We discuss remedies such as education programs and government incentives that may reduce entanglements.

  5. Thoracic auscultation in captive bottlenose dolphins (Tursiops truncatus), California sea lions (Zalophus californianus), and South African fur seals (Arctocephalus pusillus) with an electronic stethoscope.

    PubMed

    Scharpegge, Julia; Hartmann, Manuel García; Eulenberger, Klaus

    2012-06-01

    Thoracic auscultation is an important diagnostic method used in cases of suspected pulmonary disease in many species, as respiratory sounds contain significant information on the physiology and pathology of the lungs and upper airways. Respiratory diseases are frequent in marine mammals and are often listed as one of their main causes of death. The aim of this study was to investigate and report baseline parameters for the electronic-mediated thoracic auscultation of one cetacean species and two pinniped species in captivity. Respiratory sounds from 20 captive bottlenose dolphins (Tursiops truncatus), 6 California sea lions (Zalophus californianus), and 5 South African fur seals (Arctocephalus pusillus) were recorded with an electronic stethoscope. The sounds were analyzed for duration of the respiratory cycle, adventitious sounds, and peak frequencies of recorded sounds during expiration and inspiration as well as for sound intensity as reflected by waveform amplitude during the respiratory cycle. In respiratory cycles of the bottlenose dolphins' expiring "on command," the duration of the expiration was significantly shorter than the duration of the inspiration. In the examined pinnipeds of this study, there was no clear pattern concerning the duration of one breathing phase: Adventitious sounds were detected most often in bottlenose dolphins that were expiring on command and could be compared with "forced expiratory wheezes" in humans. This is the first report of forced expiratory wheezes in bottlenose dolphins; they can easily be misinterpreted as pathologic respiratory sounds. The peak frequencies of the respiratory sounds reached over 2,000 Hz in bottlenose dolphins and over 1,000 Hz in California sea lions and South African fur seals, but the variation of the frequency spectra was very high in all animals. To the authors' knowledge, this is the first systematic analysis of respiratory sounds of bottlenose dolphins and two species of pinnipeds.

  6. 75 FR 5056 - Taking and Importing Marine Mammals; Taking Marine Mammals Incidental to Space Vehicle and Test...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... vitulina richardsi), northern elephant seals (Mirounga angustirostris), California sea lions (Zalophus californianus), and northern fur seals (Callorhinus ursinus), by harassment, incidental to missile and...

  7. Identification of Prey Captures in Australian Fur Seals (Arctocephalus pusillus doriferus) Using Head-Mounted Accelerometers: Field Validation with Animal-Borne Video Cameras

    PubMed Central

    Volpov, Beth L.; Hoskins, Andrew J.; Battaile, Brian C.; Viviant, Morgane; Wheatley, Kathryn E.; Marshall, Greg; Abernathy, Kyler; Arnould, John P. Y.

    2015-01-01

    This study investigated prey captures in free-ranging adult female Australian fur seals (Arctocephalus pusillus doriferus) using head-mounted 3-axis accelerometers and animal-borne video cameras. Acceleration data was used to identify individual attempted prey captures (APC), and video data were used to independently verify APC and prey types. Results demonstrated that head-mounted accelerometers could detect individual APC but were unable to distinguish among prey types (fish, cephalopod, stingray) or between successful captures and unsuccessful capture attempts. Mean detection rate (true positive rate) on individual animals in the testing subset ranged from 67-100%, and mean detection on the testing subset averaged across 4 animals ranged from 82-97%. Mean False positive (FP) rate ranged from 15-67% individually in the testing subset, and 26-59% averaged across 4 animals. Surge and sway had significantly greater detection rates, but also conversely greater FP rates compared to heave. Video data also indicated that some head movements recorded by the accelerometers were unrelated to APC and that a peak in acceleration variance did not always equate to an individual prey item. The results of the present study indicate that head-mounted accelerometers provide a complementary tool for investigating foraging behaviour in pinnipeds, but that detection and FP correction factors need to be applied for reliable field application. PMID:26107647

  8. Death of a South American fur seal (Arctocephalus australis) after the ingestion of toads--evaluation of toad poisoning by toxicological analysis.

    PubMed

    Toennes, Stefan W; Peters, Martin; Osmann, Christine; Pogoda, Werner; Mebs, Dietrich

    2015-01-01

    Animals in zoological gardens are at risk of severe and even lethal poisoning when they accidentally ingest toads. Here we report the case of an eleven month old male South American fur seal (Arctocephalus australis) which was found dead in its outdoor enclosure in the zoo of Dortmund, Germany. Autopsy revealed the presence of two adult, partly digested common toads (Bufo bufo) in the stomach. Toxicological analysis of the stomach content using high performance liquid chromatography coupled to time-of-flight mass spectrometry (LC-TOF MS) proved the presence of bufadienolides, the major cardiotoxic components of toad poisons. Using electrochemical luminescens immunoassay (ECLIA) compounds equivalent to digitoxin were detected in the blood sample confirming the absorption of toad poison components from the intestines into the circulation potentially leading to cardiac failure. In zoological gardens special precautions are necessary to protect non-native animals from encountering toads and the risk of poisoning, particularly in early spring, the spawning period of the toads.

  9. Identification of Prey Captures in Australian Fur Seals (Arctocephalus pusillus doriferus) Using Head-Mounted Accelerometers: Field Validation with Animal-Borne Video Cameras.

    PubMed

    Volpov, Beth L; Hoskins, Andrew J; Battaile, Brian C; Viviant, Morgane; Wheatley, Kathryn E; Marshall, Greg; Abernathy, Kyler; Arnould, John P Y

    2015-01-01

    This study investigated prey captures in free-ranging adult female Australian fur seals (Arctocephalus pusillus doriferus) using head-mounted 3-axis accelerometers and animal-borne video cameras. Acceleration data was used to identify individual attempted prey captures (APC), and video data were used to independently verify APC and prey types. Results demonstrated that head-mounted accelerometers could detect individual APC but were unable to distinguish among prey types (fish, cephalopod, stingray) or between successful captures and unsuccessful capture attempts. Mean detection rate (true positive rate) on individual animals in the testing subset ranged from 67-100%, and mean detection on the testing subset averaged across 4 animals ranged from 82-97%. Mean False positive (FP) rate ranged from 15-67% individually in the testing subset, and 26-59% averaged across 4 animals. Surge and sway had significantly greater detection rates, but also conversely greater FP rates compared to heave. Video data also indicated that some head movements recorded by the accelerometers were unrelated to APC and that a peak in acceleration variance did not always equate to an individual prey item. The results of the present study indicate that head-mounted accelerometers provide a complementary tool for investigating foraging behaviour in pinnipeds, but that detection and FP correction factors need to be applied for reliable field application.

  10. Gross and microscopic visceral anatomy of the male Cape fur seal, Arctocephalus pusillus pusillus (Pinnipedia: Otariidae), with reference to organ size and growth

    PubMed Central

    STEWARDSON, CAROLYN L.; HEMSLEY, SUSAN; MEYER, MIKE A.; CANFIELD, PAUL J.; MAINDONALD, JOHN H.

    1999-01-01

    The gross and microscopic anatomy of the Cape fur seal heart, lung, liver, spleen, stomach, intestine and kidneys (n = 31 seals) is described. Absolute and relative size of organs from 30 male seals are presented, with histological examination conducted on 7 animals. The relationship between log body weight, log organ weight and age was investigated using linear regression. Twenty five animals were of known age, while 6 were aged from counts of incremental lines observed in the dentine of tooth sections. For the range of ages represented in this study, body weight changes were accurately described by the exponential growth equation, weight = wort, with body weight increasing by 23% per annum until at least 9–10 y of age. Organ weight increased at a rate of between 25% and 33% per annum until at least 9–10 y of age, with the exception of the intestines, where exponential increase appeared to have ceased by about 7 y. The relationship between body weight and organ weight was investigated using logarithmic transformations of the allometric equation, y = axb, where the exponent b is 1 if organ weight is proportional to body weight. Most organs increased in proportion to the body. However, the heart, liver and spleen had exponents b > 1, suggesting that these organs increased at a faster rate than the body. The basic anatomical features of the viscera were similar to those of other pinnipeds, with some exceptions, including the arrangement of the multilobed lung and liver. Apart from the large liver and kidneys, relative size of the organs did not differ greatly from similar sized terrestrial carnivores. The histological features of the organs were generally consistent with those previously described for this species and other otariids. The heart, as in other pinnipeds, was unlike that of cetacea in not having unusually thick endocardium or prominent Purkinje cells. Notable histological features of the lungs included prominent fibrous septa, prominent smooth muscle

  11. Measure Guideline: Summary of Interior Ducts in New Construction, Including an Efficient, Affordable Method to Install Fur-Down Interior Ducts

    SciTech Connect

    Beal, D.; McIlvaine, J.; Fonorow, K.; Martin, E.

    2011-11-01

    This document illustrates guidelines for the efficient installation of interior duct systems in new housing, including the fur-up chase method, the fur-down chase method, and interior ducts positioned in sealed attics or sealed crawl spaces.

  12. Lung Mechanics in Marine Mammals

    DTIC Science & Technology

    2014-09-30

    Pv = Phoca vitulina, Ma = Mirounga angustirostris; Cu = Callorhinus ursinus; Zc = Zalophus californianus ; Ej = Eumetopias jubatus). *Ptp all < 10cmH2O...Eumetopias jubatus), California sea lion (Zc = Zalophus californianus ), Northern fur seal (Cu = Callorhinus ursinus), harbor seal (Pv = Phoca vitulina) and...captive Steller sea lions (Ej = Eumetopias jubatus), California sea lion (Zc = Zalophus californianus ), Northern fur seal (Cu = Callorhinus ursinus

  13. Final Environmental Assessment for Hypersonic Technology Vehicle 2 Flight Tests

    DTIC Science & Technology

    2009-04-28

    Callorhinus ursinus MMPA Guadalupe fur seal Arctocephalus townsendi T California sea lion Zalophus californianus MMPA Pacific harbor seal Phoca vitulina...sparrow Spizella atrogularis SOC - Scrub habitats Mammals (includes near-shore waters) California sea lion Zalophus californianus MMPA - Coastal

  14. Impact of the 2015 El Niño-Southern Oscillation on the Abundance and Foraging Habits of Guadalupe Fur Seals and California Sea Lions from the San Benito Archipelago, Mexico

    PubMed Central

    Elorriaga-Verplancken, Fernando R.; Sierra-Rodríguez, Gema E.; Rosales-Nanduca, Hiram; Acevedo-Whitehouse, Karina; Sandoval-Sierra, Julieta

    2016-01-01

    The abundance of California sea lions (Zalophus californianus) (CSLs) and Guadalupe fur seals (Arctocephalus philippii townsendi) (GFSs) from the San Benito Archipelago (SBA) was determined through nine monthly surveys in 2014–2015. Assessment of their foraging habits was examined based on the isotopic analysis of pups (maternal indicators) (SIAR/SIBER-R). Environmental variability between 2014 and 2015 was also analyzed, in terms of sea surface temperature (SST) and chlorophyll (Chl-a) concentration. Both otariids reached their highest abundance in July of both years; however, relative to 2014, the 2015 survey showed a 59.7% decline in the total GFS abundance and a 42.9% decrease of GFS pups, while total CSL abundance decreased 52.0% and CSL pup presence decreased in 61.7%. All monthly surveys for both otariids showed a similar trend (>50% decrease in 2015). Compared to 2014, the 2015 GFSs isotopic niche was three times larger (2.0 in 2015, 0.6 in 2014) and the δ13C was significantly lower. CSLs also showed significantly lower δ13C and higher δ15N in 2015. Interannual segregation was greater for CSLs, and their pup body mass was also significantly lower during the 2015 breeding season (mean = 8.7 kg) than in the same season of 2014 (mean = 9.9 kg). The decrease in δ13C for both otariids reflected a more oceanic foraging; most likely associated with the decline in primary productivity in surrounding areas to the SBA, related to a higher SST caused by the 2015 ENSO, with a subsequent increase in foraging effort. These would explain the fewer observed individuals on land, especially pups, which showed diminished body condition (CSLs). This study highlights the importance of marine mammals as sentinel species that respond dynamically to changes in environment, providing valuable information on the effect of ENSO on pinnipeds in Mexican waters. PMID:27171473

  15. Impact of the 2015 El Niño-Southern Oscillation on the Abundance and Foraging Habits of Guadalupe Fur Seals and California Sea Lions from the San Benito Archipelago, Mexico.

    PubMed

    Elorriaga-Verplancken, Fernando R; Sierra-Rodríguez, Gema E; Rosales-Nanduca, Hiram; Acevedo-Whitehouse, Karina; Sandoval-Sierra, Julieta

    2016-01-01

    The abundance of California sea lions (Zalophus californianus) (CSLs) and Guadalupe fur seals (Arctocephalus philippii townsendi) (GFSs) from the San Benito Archipelago (SBA) was determined through nine monthly surveys in 2014-2015. Assessment of their foraging habits was examined based on the isotopic analysis of pups (maternal indicators) (SIAR/SIBER-R). Environmental variability between 2014 and 2015 was also analyzed, in terms of sea surface temperature (SST) and chlorophyll (Chl-a) concentration. Both otariids reached their highest abundance in July of both years; however, relative to 2014, the 2015 survey showed a 59.7% decline in the total GFS abundance and a 42.9% decrease of GFS pups, while total CSL abundance decreased 52.0% and CSL pup presence decreased in 61.7%. All monthly surveys for both otariids showed a similar trend (>50% decrease in 2015). Compared to 2014, the 2015 GFSs isotopic niche was three times larger (2.0 in 2015, 0.6 in 2014) and the δ13C was significantly lower. CSLs also showed significantly lower δ13C and higher δ15N in 2015. Interannual segregation was greater for CSLs, and their pup body mass was also significantly lower during the 2015 breeding season (mean = 8.7 kg) than in the same season of 2014 (mean = 9.9 kg). The decrease in δ13C for both otariids reflected a more oceanic foraging; most likely associated with the decline in primary productivity in surrounding areas to the SBA, related to a higher SST caused by the 2015 ENSO, with a subsequent increase in foraging effort. These would explain the fewer observed individuals on land, especially pups, which showed diminished body condition (CSLs). This study highlights the importance of marine mammals as sentinel species that respond dynamically to changes in environment, providing valuable information on the effect of ENSO on pinnipeds in Mexican waters.

  16. Environmental Assessment for Airborne Laser Debris Management Vandenberg Air Force Base, California

    DTIC Science & Technology

    2008-07-01

    macrocephalus) has been designated as "strategic." The California sea lion ( Zalophus californianus ), northern fur seal (Callorhinus ursinus), northern...northern elephant seal (Mirounga angustirostris), California sea lion ( Zalophus californianus ), and northern fur seal (Callorhinus ursinus). These four...feet (150 to 800 m) to feed and spend 2 to 3 minutes on the surface after dives lasting 21 to 25 minutes. California Sea Lion ( Zalophus californianus

  17. Final Environmental Assessment/Overseas Environmental Assessment Joint Strile Fighter System Development and Demonstration Developmental Test Program

    DTIC Science & Technology

    2007-01-01

    seal (Phoca vitulina) Y Y Y Y Northern elephant seal (Mirounga angustirostris) Y Y Y Y California sea lion ( Zalophus californianus ) Y Y Y Y...Harp seal A A A A Halichoerus grypus gryous Gray seal A A A A Cystophora cristata Hooded seal A A A A Zalophus californianus ...California sea lion (Salophus californianus ), and the northern fur seal (Callorhinus ursinus). The Federally-protected Guadalupe fur seal (Arctocehalus

  18. 16 CFR 301.13 - Fur products having furs with different countries of origin.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Fur products having furs with different... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.13 Fur products having furs with different countries of origin. When a fur product is composed of furs...

  19. 16 CFR 301.37 - Manner of invoicing furs and fur products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Manner of invoicing furs and fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.37 Manner of invoicing furs and fur products. (a) In the invoicing of furs and fur products, all of the required...

  20. 16 CFR 301.38 - Advertising of furs and fur products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Advertising of furs and fur products. 301.38... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.38 Advertising of furs and fur products. (a)(1) In advertising furs or fur products, all parts of the required information shall be...

  1. 16 CFR 301.13 - Fur products having furs with different countries of origin.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Fur products having furs with different... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.13 Fur products having furs with different countries of origin. When a fur product is composed of furs...

  2. 16 CFR 301.13 - Fur products having furs with different countries of origin.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Fur products having furs with different... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.13 Fur products having furs with different countries of origin. When a fur product is composed of furs...

  3. 16 CFR 301.18 - Passing off domestic furs as imported furs prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Passing off domestic furs as imported furs... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.18 Passing off domestic furs as imported furs prohibited. No domestic furs nor fur products shall be labeled, invoiced...

  4. 16 CFR 301.37 - Manner of invoicing furs and fur products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Manner of invoicing furs and fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.37 Manner of invoicing furs and fur products. (a) In the invoicing of furs and fur products, all of the required...

  5. 16 CFR 301.13 - Fur products having furs with different countries of origin.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Fur products having furs with different... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.13 Fur products having furs with different countries of origin. When a fur product is composed of furs...

  6. 16 CFR 301.38 - Advertising of furs and fur products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Advertising of furs and fur products. 301.38... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.38 Advertising of furs and fur products. (a)(1) In advertising furs or fur products, all parts of the required information shall be...

  7. 16 CFR 301.18 - Passing off domestic furs as imported furs prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Passing off domestic furs as imported furs... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.18 Passing off domestic furs as imported furs prohibited. No domestic furs nor fur products shall be labeled, invoiced...

  8. 16 CFR 301.37 - Manner of invoicing furs and fur products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Manner of invoicing furs and fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.37 Manner of invoicing furs and fur products. (a) In the invoicing of furs and fur products, all of the required...

  9. 16 CFR 301.38 - Advertising of furs and fur products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Advertising of furs and fur products. 301.38... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.38 Advertising of furs and fur products. (a)(1) In advertising furs or fur products, all parts of the required information shall be...

  10. 16 CFR 301.18 - Passing off domestic furs as imported furs prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Passing off domestic furs as imported furs... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.18 Passing off domestic furs as imported furs prohibited. No domestic furs nor fur products shall be labeled, invoiced...

  11. 16 CFR 301.37 - Manner of invoicing furs and fur products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Manner of invoicing furs and fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.37 Manner of invoicing furs and fur products. (a) In the invoicing of furs and fur products, all of the required...

  12. 16 CFR 301.13 - Fur products having furs with different countries of origin.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Fur products having furs with different... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.13 Fur products having furs with different countries of origin. When a fur product is composed of furs...

  13. 16 CFR 301.38 - Advertising of furs and fur products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Advertising of furs and fur products. 301.38... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.38 Advertising of furs and fur products. (a)(1) In advertising furs or fur products, all parts of the required information shall be...

  14. 16 CFR 301.18 - Passing off domestic furs as imported furs prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Passing off domestic furs as imported furs... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.18 Passing off domestic furs as imported furs prohibited. No domestic furs nor fur products shall be labeled, invoiced...

  15. 16 CFR 301.37 - Manner of invoicing furs and fur products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Manner of invoicing furs and fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.37 Manner of invoicing furs and fur products. (a) In the invoicing of furs and fur products, all of the required...

  16. Seals and Sealing Techniques

    NASA Technical Reports Server (NTRS)

    1972-01-01

    Developments by the aerospace industry in seals and sealing techniques are announced for possible use in other areas. The announcements presented are grouped as: sealing techniques for cryogenic fluids, high pressure applications, and modification for improved performance.

  17. Tuberculosis in wild seals and characterisation of the seal bacillus.

    PubMed

    Cousins, D V; Williams, S N; Reuter, R; Forshaw, D; Chadwick, B; Coughran, D; Collins, P; Gales, N

    1993-03-01

    Tuberculosis was diagnosed in 3 otariid seals found dead on beaches at 3 locations on the south coast of Western Australian between May 1990 and March 1991. This confirms that tuberculosis is present in the 2 native seals (Neophoca cinerea and Arctocephalus forsteri) in Western Australian waters. Mycobacterium sp isolated from the lungs of 2 of the seals were studied to determine the similarity of the strains to each other, to the strains isolated during 1986 from Australian sea lions and New Zealand fur seals kept in captivity at a marine park near Perth, Western Australia, and to a strain isolated in 1988 from a seal trainer who worked with the infected captive seals for 3 years. After restriction endonuclease analysis (REA) with the endonucleases Bst EII, Bcl I and Pvu II, one of the wild seal strains appeared to have identical DNA fragment patterns to the strains from the captive seals and the seal trainer. The other wild seal isolate had identical REA profiles using Bst EII and Bcl I, but a minor difference was detected using Pvu II. Differences in these isolates were more clearly seen in restriction fragment length polymorphisms after hybridisation with two DNA probes. The secretory protein MPB70, present in M bovis, was not detected in wild seal isolates using sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blotting techniques. Analysis of protein and DNA fragment profiles indicated that seal tuberculosis isolates form a unique cluster within the M tuberculosis complex.

  18. 16 CFR 301.39 - Exempted fur products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Exempted fur products. 301.39 Section 301.39... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.39 Exempted fur products. (a) If the cost of any fur trim or other manufactured fur or furs contained in a fur product, exclusive of any costs...

  19. 16 CFR 301.39 - Exempted fur products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Exempted fur products. 301.39 Section 301.39... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.39 Exempted fur products. (a) If the cost of any fur trim or other manufactured fur or furs contained in a fur product, exclusive of any costs...

  20. 16 CFR 301.39 - Exempted fur products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Exempted fur products. 301.39 Section 301.39... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.39 Exempted fur products. (a) If the cost of any fur trim or other manufactured fur or furs contained in a fur product, exclusive of any costs...

  1. 16 CFR 301.39 - Exempted fur products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Exempted fur products. 301.39 Section 301.39... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.39 Exempted fur products. (a) If the cost of any fur trim or other manufactured fur or furs contained in a fur product, exclusive of any costs...

  2. 16 CFR 301.39 - Exempted fur products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Exempted fur products. 301.39 Section 301.39... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.39 Exempted fur products. (a) If the cost of any fur trim or other manufactured fur or furs contained in a fur product, exclusive of any costs...

  3. Environmental Assessment/Overseas Environmental Assessment for the F-35 Joint Strike Fighter Initial Operational Test and Evaluation

    DTIC Science & Technology

    2009-09-01

    Sierra Nevada yellow-legged frog Candidate Birds Coccyzus americanus occidentalis Western yellow-billed cuckoo Candidate Gymnogyps californianus ...abundant species include the harbor seal (Phoca vitulina), northern elephant seal (Mirounga angustirostris), California sea lion ( Zalophus ... californianus ), and northern fur seal (Callorhinus ursinus). These four species breed on land within the Sea Range. The southern sea otter (Enhydra lutris

  4. Environmental Assessment for Conventional Strike Missile Demonstration

    DTIC Science & Technology

    2010-08-01

    Pinnipeds that may be found onshore (“hauled-out”) within the ROI include the California sea lion ( Zalophus californianus ) and Pacific harbor seal...Callorhinus ursinus MMPA Guadalupe fur seal Arctocephalus townsendi T California sea lion Zalophus californianus MMPA Pacific harbor seal Phoca

  5. The Seal Killing Controversy: What Are the Facts?

    ERIC Educational Resources Information Center

    Scheffer, Victor B.

    1973-01-01

    Discusses the seal controversy using the harp and Alaska fur seals to illustrate the two distinct issues, i.e., conservation (the effect of killing upon the animal population); and two, morality (the effect of killing upon the human spirit). Factual information combines with personal philosophy. (LK)

  6. Field Lines of Flying Fur.

    ERIC Educational Resources Information Center

    Lapp, David R.

    1996-01-01

    Presents a demonstration that uses insulated conductors and hairs clipped from rabbit fur to help students visualize the electric field in three dimensions and motivate them to try their own variations. (JRH)

  7. 16 CFR 301.36 - Sectional fur products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...; as for example: Dyed Rabbit Fur origin: France Trimming: Dyed Mouton-processed Lamb Fur origin: Argentina or Body: Dyed Kolinsky Fur origin: Russia Tail: Dyed Mink Fur origin: Canada (b) The provisions...

  8. 16 CFR 301.36 - Sectional fur products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...; as for example: Dyed Rabbit Fur origin: France Trimming: Dyed Mouton-processed Lamb Fur origin: Argentina or Body: Dyed Kolinsky Fur origin: Russia Tail: Dyed Mink Fur origin: Canada (b) The provisions...

  9. 16 CFR 301.36 - Sectional fur products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...; as for example: Dyed Rabbit Fur origin: France Trimming: Dyed Mouton-processed Lamb Fur origin: Argentina or Body: Dyed Kolinsky Fur origin: Russia Tail: Dyed Mink Fur origin: Canada (b) The provisions...

  10. 16 CFR 301.36 - Sectional fur products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...; as for example: Dyed Rabbit Fur origin: France Trimming: Dyed Mouton-processed Lamb Fur origin: Argentina or Body: Dyed Kolinsky Fur origin: Russia Tail: Dyed Mink Fur origin: Canada (b) The provisions...

  11. 16 CFR 301.36 - Sectional fur products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...; as for example: Dyed Rabbit Fur origin: France Trimming: Dyed Mouton-processed Lamb Fur origin: Argentina or Body: Dyed Kolinsky Fur origin: Russia Tail: Dyed Mink Fur origin: Canada (b) The provisions...

  12. Final Environmental Assessment for Conventional Strike Missile Demonstration

    DTIC Science & Technology

    2010-08-11

    Vandenberg AFB to rest, molt, and/or breed. Pinnipeds that may be found onshore (“hauled-out”) within the ROI include the California sea lion ( Zalophus ... californianus ) and Pacific harbor seal (Phoca vitulina richardsi). None of these species are listed as endangered or threatened, but all receive Federal...Federal Status Pinnipeds Northern fur seal Callorhinus ursinus MMPA Guadalupe fur seal Arctocephalus townsendi T California sea lion Zalophus

  13. 76 FR 13550 - Fur Products Labeling Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... less without a fur-content label. B. TFLA On December 18, 2010, the President signed TFLA into law... concerning consumer perception of the fur names required by the Name Guide. Does this evidence indicate...

  14. Acoustic seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor)

    2006-01-01

    The invention relates to a sealing device having an acoustic resonator. The acoustic resonator is adapted to create acoustic waveforms to generate a sealing pressure barrier blocking fluid flow from a high pressure area to a lower pressure area. The sealing device permits noncontacting sealing operation. The sealing device may include a resonant-macrosonic-synthesis (RMS) resonator.

  15. Acoustic Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor)

    2006-01-01

    The invention relates to a sealing device having an acoustic resonator. The acoustic resonator is adapted to create acoustic waveforms to generate a sealing pressure barrier blocking fluid flow from a high pressure area to a lower pressure area. The sealing device permits noncontacting sealing operation. The sealing device may include a resonant-macrosonic-synthesis (RMS) resonator.

  16. 50 CFR 217.62 - Permissible methods of taking.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...)—31,161; (2) California sea lions (Zalophus californianus)—465,129; (3) Northern elephant seals (Mirounga angustirostris)—80,024; (4) Northern fur seals (Callorhinus ursinus)—62,500; and (5) Steller sea lions (Eumetopias jubatus)—1,824....

  17. Environmental Assessment for Hypersonic Technology Vehicle 2 Flight Tests

    DTIC Science & Technology

    2009-04-01

    Callorhinus ursinus MMPA Guadalupe fur seal Arctocephalus townsendi T California sea lion Zalophus californianus MMPA Pacific harbor seal Phoca vitulina...Spizella atrogularis SOC - Scrub habitats Mammals (includes near-shore waters) California sea lion Zalophus californianus MMPA - Coastal waters and

  18. 50 CFR 218.171 - Permissible methods of taking.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... MAMMALS Taking Marine Mammals Incidental to U.S. Navy Research, Development, Test, and Evaluation...); (2) Northern fur seal (Callorhinus ursinus)—220 (an average of 44 annually); (3) California sea lion (Zalophus californianus)—570 (an average of 114 annually); (4) Northern elephant seal...

  19. 50 CFR 218.171 - Permissible methods of taking.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... MAMMALS Taking Marine Mammals Incidental to U.S. Navy Research, Development, Test, and Evaluation...); (2) Northern fur seal (Callorhinus ursinus)—220 (an average of 44 annually); (3) California sea lion (Zalophus californianus)—570 (an average of 114 annually); (4) Northern elephant seal...

  20. 50 CFR 218.171 - Permissible methods of taking.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... MAMMALS Taking Marine Mammals Incidental to U.S. Navy Research, Development, Test, and Evaluation...); (2) Northern fur seal (Callorhinus ursinus)—220 (an average of 44 annually); (3) California sea lion (Zalophus californianus)—570 (an average of 114 annually); (4) Northern elephant seal...

  1. 50 CFR 218.171 - Permissible methods of taking.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... MAMMALS Taking Marine Mammals Incidental to U.S. Navy Research, Development, Test, and Evaluation...); (2) Northern fur seal (Callorhinus ursinus)—220 (an average of 44 annually); (3) California sea lion (Zalophus californianus)—570 (an average of 114 annually); (4) Northern elephant seal...

  2. 19 CFR 12.63 - Seal-skin or sea-otter-skin waste.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Seal-skin or sea-otter-skin waste. 12.63 Section... OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.63 Seal-skin or sea-otter-skin waste. Seal-skin or sea-otter-skin waste composed of small pieces not large enough to...

  3. 19 CFR 12.63 - Seal-skin or sea-otter-skin waste.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Seal-skin or sea-otter-skin waste. 12.63 Section... OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.63 Seal-skin or sea-otter-skin waste. Seal-skin or sea-otter-skin waste composed of small pieces not large enough to...

  4. 19 CFR 12.63 - Seal-skin or sea-otter-skin waste.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Seal-skin or sea-otter-skin waste. 12.63 Section... OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.63 Seal-skin or sea-otter-skin waste. Seal-skin or sea-otter-skin waste composed of small pieces not large enough to...

  5. 19 CFR 12.63 - Seal-skin or sea-otter-skin waste.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Seal-skin or sea-otter-skin waste. 12.63 Section... OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.63 Seal-skin or sea-otter-skin waste. Seal-skin or sea-otter-skin waste composed of small pieces not large enough to...

  6. 19 CFR 12.63 - Seal-skin or sea-otter-skin waste.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Seal-skin or sea-otter-skin waste. 12.63 Section... OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.63 Seal-skin or sea-otter-skin waste. Seal-skin or sea-otter-skin waste composed of small pieces not large enough to...

  7. Glass sealing

    SciTech Connect

    Brow, R.K.; Kovacic, L.; Chambers, R.S.

    1996-04-01

    Hernetic glass sealing technologies developed for weapons component applications can be utilized for the design and manufacture of fuel cells. Design and processing of of a seal are optimized through an integrated approach based on glass composition research, finite element analysis, and sealing process definition. Glass sealing procedures are selected to accommodate the limits imposed by glass composition and predicted calculations.

  8. 16 CFR 301.22 - Disclosure of damaged furs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Disclosure of damaged furs. 301.22 Section... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.22 Disclosure of damaged furs. (a) The term damaged fur, as used in this part, means a fur, which, because of a known or patent...

  9. 16 CFR 301.22 - Disclosure of damaged furs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Disclosure of damaged furs. 301.22 Section... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.22 Disclosure of damaged furs. (a) The term damaged fur, as used in this part, means a fur, which, because of a known or patent...

  10. 16 CFR 301.20 - Fur products composed of pieces.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Fur products composed of pieces. 301.20... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.20 Fur products composed of pieces. (a) Where fur products, or fur mats and plates, are composed in whole or in substantial part...

  11. 16 CFR 301.22 - Disclosure of damaged furs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Disclosure of damaged furs. 301.22 Section... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.22 Disclosure of damaged furs. (a) The term damaged fur, as used in this part, means a fur, which, because of a known or patent...

  12. 16 CFR 301.20 - Fur products composed of pieces.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Fur products composed of pieces. 301.20... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.20 Fur products composed of pieces. (a) Where fur products, or fur mats and plates, are composed in whole or in substantial part...

  13. 16 CFR 301.20 - Fur products composed of pieces.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Fur products composed of pieces. 301.20... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.20 Fur products composed of pieces. (a) Where fur products, or fur mats and plates, are composed in whole or in substantial part...

  14. 16 CFR 301.20 - Fur products composed of pieces.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Fur products composed of pieces. 301.20... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.20 Fur products composed of pieces. (a) Where fur products, or fur mats and plates, are composed in whole or in substantial part...

  15. 16 CFR 301.22 - Disclosure of damaged furs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Disclosure of damaged furs. 301.22 Section... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.22 Disclosure of damaged furs. (a) The term damaged fur, as used in this part, means a fur, which, because of a known or patent...

  16. 16 CFR 301.20 - Fur products composed of pieces.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Fur products composed of pieces. 301.20... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.20 Fur products composed of pieces. (a) Where fur products, or fur mats and plates, are composed in whole or in substantial part...

  17. 16 CFR 301.22 - Disclosure of damaged furs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Disclosure of damaged furs. 301.22 Section... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.22 Disclosure of damaged furs. (a) The term damaged fur, as used in this part, means a fur, which, because of a known or patent...

  18. Coining seal

    DOEpatents

    Mancebo, Lloyd

    1976-01-01

    A bakeable high pressure-vacuum seal is provided in which an inductile sealing element having a butterfly shaped crosssection with protruding sharp edges at each of the four corners, is sandwiched between two ductile sealing elements, the sandwiched assembly then being compressed between the surfaces of the flange elements of a high pressure or high vacuum vessel to coin the ductile sealing element into the surface of the inductile sealing element as well as the surfaces of the flange elements.

  19. Sealing device

    SciTech Connect

    Garcia-Crespo, Andres Jose

    2013-12-10

    A sealing device for sealing a gap between a dovetail of a bucket assembly and a rotor wheel is disclosed. The sealing device includes a cover plate configured to cover the gap and a retention member protruding from the cover plate and configured to engage the dovetail. The sealing device provides a seal against the gap when the bucket assemply is subjected to a centrifugal force.

  20. Building America Case Study: Duct in Conditioned Space in a Dropped Ceiling or Fur-down, Gainesville, Florida (Fact Sheet)

    SciTech Connect

    Not Available

    2014-09-01

    Forced air distribution systems (duct systems) typically are installed out of sight for aesthetic reasons, most often in unconditioned areas such as an attic or crawlspace. Any leakage of air to or from the duct system (duct leakage) in unconditioned space not only loses energy, but impacts home and equipment durability and indoor air quality. An obvious solution to this problem is to bring the duct system into the interior of the house, either by sealing the area where the ducts are installed (sealed attic or crawlspace) or by building an interior cavity or chase above the ceiling plane (raised ceiling or fur-up chase) or below the ceiling plane (dropped ceiling or fur-down) for the duct system. This case study examines one Building America builder partner's implementation of an inexpensive, quick and effective method of building a fur-down or dropped ceiling chase.

  1. 16 CFR 301.18 - Passing off domestic furs as imported furs prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Passing off domestic furs as imported furs prohibited. 301.18 Section 301.18 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.18 Passing...

  2. Pet fur or fake fur? A forensic approach

    PubMed Central

    2014-01-01

    Background In forensic science there are many types of crime that involve animals. Therefore, the identification of the species has become an essential investigative tool. The exhibits obtained from such offences are very often a challenge for forensic experts. Indeed, most biological materials are traces, hair or tanned fur. With hair samples, a common forensic approach should proceed from morphological and structural microscopic examination to DNA analysis. However, the microscopy of hair requires a lot of experience and a suitable comparative database to be able to recognize with a high degree of accuracy that a sample comes from a particular species and then to determine whether it is a protected one. DNA analysis offers the best opportunity to answer the question, ‘What species is this?’ In our work, we analyzed different samples of fur coming from China used to make hats and collars. Initially, the samples were examined under a microscope, then the mitochondrial DNA was tested for species identification. For this purpose, the genetic markers used were the 12S and 16S ribosomal RNA, while the hypervariable segment I of the control region was analyzed afterwards, to determine whether samples belonged to the same individual. Results Microscopic examination showed that the fibres were of animal origin, although it was difficult to determine with a high degree of confidence which species they belonged to and if they came from a protected species. Therefore, DNA analysis was essential to try to clarify the species of these fur samples. Conclusions Macroscopic and microscopic analysis confirmed the hypothesis regarding the analyzed hair belonging to real animals, although it failed to prove with any kind of certainty which actual family it came from, therefore, the species remains unknown. Sequence data analysis and comparisons with the samples available in GenBank showed that the hair, in most cases, belonged to the Canidae family, and in one case only to

  3. Security seal

    DOEpatents

    Gobeli, Garth W.

    1985-01-01

    Security for a package or verifying seal in plastic material is provided by a print seal with unique thermally produced imprints in the plastic. If tampering is attempted, the material is irreparably damaged and thus detectable. The pattern of the imprints, similar to "fingerprints" are recorded as a positive identification for the seal, and corresponding recordings made to allow comparison. The integrity of the seal is proved by the comparison of imprint identification records made by laser beam projection.

  4. α-fur, an antisense RNA gene to fur in the extreme acidophile Acidithiobacillus ferrooxidans.

    PubMed

    Lefimil, C; Jedlicki, E; Holmes, D S

    2014-03-01

    A large non-coding RNA, termed α-Fur, of ~1000 nt has been detected in the extreme acidophile Acidithiobacillus ferrooxidans encoded on the antisense strand to the iron-responsive master regulator fur (ferric uptake regulator) gene. A promoter for α-fur was predicted bioinformatically and validated using gene fusion experiments. The promoter is situated within the coding region and in the same sense as proB, potentially encoding a glutamate 5-kinase. The 3' termination site of the α-fur transcript was determined by 3' rapid amplification of cDNA ends to lie 7 nt downstream of the start of transcription of fur. Thus, α-fur is antisense to the complete coding region of fur, including its predicted ribosome-binding site. The genetic context of α-fur is conserved in several members of the genus Acidithiobacillus but not in all acidophiles, indicating that it is monophyletic but not niche specific. It is hypothesized that α-Fur regulates the cellular level of Fur. This is the fourth example of an antisense RNA to fur, although it is the first in an extreme acidophile, and underscores the growing importance of cis-encoded non-coding RNAs as potential regulators involved in the microbial iron-responsive stimulon.

  5. Pet fur color and texture classification

    NASA Astrophysics Data System (ADS)

    Yen, Jonathan; Mukherjee, Debarghar; Lim, SukHwan; Tretter, Daniel

    2007-01-01

    Object segmentation is important in image analysis for imaging tasks such as image rendering and image retrieval. Pet owners have been known to be quite vocal about how important it is to render their pets perfectly. We present here an algorithm for pet (mammal) fur color classification and an algorithm for pet (animal) fur texture classification. Per fur color classification can be applied as a necessary condition for identifying the regions in an image that may contain pets much like the skin tone classification for human flesh detection. As a result of the evolution, fur coloration of all mammals is caused by a natural organic pigment called Melanin and Melanin has only very limited color ranges. We have conducted a statistical analysis and concluded that mammal fur colors can be only in levels of gray or in two colors after the proper color quantization. This pet fur color classification algorithm has been applied for peteye detection. We also present here an algorithm for animal fur texture classification using the recently developed multi-resolution directional sub-band Contourlet transform. The experimental results are very promising as these transforms can identify regions of an image that may contain fur of mammals, scale of reptiles and feather of birds, etc. Combining the color and texture classification, one can have a set of strong classifiers for identifying possible animals in an image.

  6. Structure and regulon of Campylobacter jejuni ferric uptake regulator Fur define apo-Fur regulation.

    PubMed

    Butcher, James; Sarvan, Sabina; Brunzelle, Joseph S; Couture, Jean-François; Stintzi, Alain

    2012-06-19

    The full regulatory potential of the ferric uptake regulator (Fur) family of proteins remains undefined despite over 20 years of study. We report herein an integrated approach that combines both genome-wide technologies and structural studies to define the role of Fur in Campylobacter jejuni (Cj). CjFur ChIP-chip assays identified 95 genomic loci bound by CjFur associated with functions as diverse as iron acquisition, flagellar biogenesis, and non-iron ion transport. Comparative analysis with transcriptomic data revealed that CjFur regulation extends beyond solely repression and also includes both gene activation and iron-independent regulation. Computational analysis revealed the presence of an elongated holo-Fur repression motif along with a divergent holo-Fur activation motif. This diversity of CjFur DNA-binding elements is supported by the crystal structure of CjFur, which revealed a unique conformation of its DNA-binding domain and the absence of metal in the regulatory site. Strikingly, our results indicate that the apo-CjFur structure retains the canonical V-shaped dimer reminiscent of previously characterized holo-Fur proteins enabling DNA interaction. This conformation stems from a structurally unique hinge domain that is poised to further contribute to CjFur's regulatory functions by modulating the orientation of the DNA-binding domain upon binding of iron. The unique features of the CjFur crystal structure rationalize the binding sequence diversity that was uncovered during ChIP-chip analysis and defines apo-Fur regulation.

  7. Detailed analysis of Helicobacter pylori Fur-regulated promoters reveals a Fur box core sequence and novel Fur-regulated genes.

    PubMed

    Pich, Oscar Q; Carpenter, Beth M; Gilbreath, Jeremy J; Merrell, D Scott

    2012-06-01

    In Helicobacter pylori, iron balance is controlled by the Ferric uptake regulator (Fur), an iron-sensing repressor protein that typically regulates expression of genes implicated in iron transport and storage. Herein, we carried out extensive analysis of Fur-regulated promoters and identified a 7-1-7 motif with dyad symmetry (5'-TAATAATnATTATTA-3'), which functions as the Fur box core sequence of H. pylori. Addition of this sequence to the promoter region of a typically non-Fur regulated gene was sufficient to impose Fur-dependent regulation in vivo. Moreover, mutation of this sequence within Fur-controlled promoters negated regulation. Analysis of the H. pylori chromosome for the occurrence of the Fur box established the existence of well-conserved Fur boxes in the promoters of numerous known Fur-regulated genes, and revealed novel putative Fur targets. Transcriptional analysis of the new candidate genes demonstrated Fur-dependent repression of HPG27_51, HPG27_52, HPG27_199, HPG27_445, HPG27_825 and HPG27_1063, as well as Fur-mediated activation of the cytotoxin associated gene A, cagA (HPG27_507). Furthermore, electrophoretic mobility shift assays confirmed specific binding of Fur to the promoters of each of these genes. Future experiments will determine whether loss of Fur regulation of any of these particular genes contributes to the defects in colonization exhibited by the H. pylori fur mutant.

  8. Seal assembly

    DOEpatents

    Johnson, Roger Neal; Longfritz, William David

    2001-01-01

    A seal assembly that seals a gap formed by a groove comprises a seal body, a biasing element, and a connection that connects the seal body to the biasing element to form the seal assembly. The seal assembly further comprises a concave-shaped center section and convex-shaped contact portions at each end of the seal body. The biasing element is formed from an elastic material and comprises a convex-shaped center section and concave-shaped biasing zones that are opposed to the convex-shaped contact portions. The biasing element is adapted to be compressed to change a width of the seal assembly from a first width to a second width that is smaller than the first width. In the compressed state, the seal assembly can be disposed in the groove. After release of the compressing force, the seal assembly expands. The contact portions will move toward a surface of the groove and the biasing zones will move into contact with another surface of the groove. The biasing zones will bias the contact portions of the seal body against the surface of the groove.

  9. 16 CFR 301.21 - Disclosure of used furs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Disclosure of used furs. 301.21 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.21 Disclosure of used furs. (a) When fur in any form has been worn or used by an ultimate consumer it shall be designated “used fur” as...

  10. 16 CFR 301.21 - Disclosure of used furs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Disclosure of used furs. 301.21 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.21 Disclosure of used furs. (a) When fur in any form has been worn or used by an ultimate consumer it shall be designated “used fur” as...

  11. 16 CFR 301.21 - Disclosure of used furs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Disclosure of used furs. 301.21 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.21 Disclosure of used furs. (a) When fur in any form has been worn or used by an ultimate consumer it shall be designated “used fur” as...

  12. 16 CFR 301.21 - Disclosure of used furs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Disclosure of used furs. 301.21 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.21 Disclosure of used furs. (a) When fur in any form has been worn or used by an ultimate consumer it shall be designated “used fur” as...

  13. 16 CFR 301.21 - Disclosure of used furs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Disclosure of used furs. 301.21 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.21 Disclosure of used furs. (a) When fur in any form has been worn or used by an ultimate consumer it shall be designated “used fur” as...

  14. Environmental Impact Analysis Process. Volume 1. Preliminary Draft Environmental Impact Statement Construction and Operation of Space Launch Complex 7, Vandenberg Air Force Base, California

    DTIC Science & Technology

    1989-04-06

    Stewart, B. S.; Perryman, W. E. 1987. Foraging characteristics of northern fur seals (Callorhinus ursinus) and California sea lions ( Zalophus ... californianus ). Abst. Seventh Biennial Conference on the Biology of Marine Mammals. p. 2. Arthur D. Little, Inc. 1984. Public draft, Point Arguello field and

  15. Environmental Assessment for Ongoing and Future Operations at U.S. Navy Dabob Bay and Hood Canal Military Operating Areas

    DTIC Science & Technology

    2007-11-02

    Zalophus californianus None Most sensitive to sounds >1 kHz, can hear to  Hz. Common resident and seasonal migrant Steller Sea Lion...jubatus), the northern fur seal (Callorhinus ursinus), and the California sea lion ( Zalophus californianus ), occur in Puget Sound (Angell and Balcomb

  16. Nozzle seal

    DOEpatents

    Groff, Russell Dennis; Vatovec, Richard John

    1978-06-11

    In an illustrative embodiment of the invention, a nuclear reactor pressure vessel, having an internal hoop from which the heated coolant emerges from the reactor core and passes through to the reactor outlet nozzles, is provided with annular sealing members operatively disposed between the outlet nozzle and the hoop and partly within a retaining annulus formed in the hoop. The sealing members are biased against the pressure vessel and the hoop and one of the sealing members is provided with a piston type pressure ring sealing member which effectively closes the path between the inlet and outlet coolants in the region about the outlet nozzle establishing a leak-proof condition. Furthermore, the flexible responsiveness of the seal assures that the seal will not structurally couple the hoop to the pressure vessel.

  17. [Asthma caused by allergy to cat fur].

    PubMed

    May, K L; Hofman, T

    2000-01-01

    Sensitivity to cats fur alergen, Fel. d. 1 is presented as the second most important cause, after allergy to mites, of perennial atopic asthma. The authors collected the data from literature concerning the concentrations of Fel. d. 1 in homes and public places. Further the structure and production of Fel d. 1 also its cross reactivity and the methods of it's elimination from the environment are described and discussed. Authors own observations of 20 cases of cats fur asthma and atopic dermatitis support the opinion that only half of the patients suspect cats as the cause of their illness and cats fur sensitivity is always accompanied by inhalant or food allergy.

  18. Technology Solutions Case Study: Duct in Conditioned Space in a Dropped Ceiling or Fur-down, Gainesville, Florida

    SciTech Connect

    2014-09-01

    Forced-air distribution systems (duct systems) typically are installed out of sight for aesthetic reasons, most often in unconditioned areas such as attics or crawlspaces. Any leakage of air to or from the duct system in unconditioned space not only loses energy, but impacts home and equipment durability and indoor air quality. An obvious solution is to bring the duct system into the interior of the house, either by sealing the area where the ducts are installed (attic or crawlspace) or by building an interior cavity or chase above the ceiling plane (raised ceiling or fur-up chase) or below the ceiling plane (dropped ceiling or fur-down) for the duct system. In this project, Building America Partnership for Improved Residential Construction team partnered with Tommy Williams Homes to implement an inexpensive, quick, and effective method of building a fur-down chase.

  19. Ferrules seals

    DOEpatents

    Smith, James L.

    1984-01-01

    A device is provided for sealing an inner tube and an outer tube without excessively deforming the tubes. The device includes two ferrules which cooperate to form a vacuum-tight seal between the inner tube and outer tube and having mating surfaces such that overtightening is not possible.

  20. Ferrules seals

    DOEpatents

    Smith, J.L.

    1984-07-10

    A device is provided for sealing an inner tube and an outer tube without excessively deforming the tubes. The device includes two ferrules which cooperate to form a vacuum-tight seal between the inner tube and outer tube and having mating surfaces such that overtightening is not possible. 3 figs.

  1. Ceramic Seal.

    SciTech Connect

    Smartt, Heidi A.; Romero, Juan A.; Custer, Joyce Olsen; Hymel, Ross W.; Krementz, Dan; Gobin, Derek; Harpring, Larry; Martinez-Rodriguez, Michael; Varble, Don; DiMaio, Jeff; Hudson, Stephen

    2016-11-01

    Containment/Surveillance (C/S) measures are critical to any verification regime in order to maintain Continuity of Knowledge (CoK). The Ceramic Seal project is research into the next generation technologies to advance C/S, in particular improving security and efficiency. The Ceramic Seal is a small form factor loop seal with improved tamper-indication including a frangible seal body, tamper planes, external coatings, and electronic monitoring of the seal body integrity. It improves efficiency through a self-securing wire and in-situ verification with a handheld reader. Sandia National Laboratories (SNL) and Savannah River National Laboratory (SRNL), under sponsorship from the U.S. National Nuclear Security Administration (NNSA) Office of Defense Nuclear Nonproliferation Research and Development (DNN R&D), have previously designed and have now fabricated and tested Ceramic Seals. Tests have occurred at both SNL and SRNL, with different types of tests occurring at each facility. This interim report will describe the Ceramic Seal prototype, the design and development of a handheld standalone reader and an interface to a data acquisition system, fabrication of the seals, and results of initial testing.

  2. Hochschule fur Film und Fernsehen (Babelsberg).

    ERIC Educational Resources Information Center

    Neumann, Roland

    1992-01-01

    Describes the Hochschule fur Film und Fernsehen, an institution of higher education for the study of film and television production in Babelsberg, Germany (formerly the German Democratic Republic). Discusses the major reorientations in the school caused by Germany's reunification. (SR)

  3. Measure Guideline: Summary of Interior Ducts in New Construction, Including an Efficient, Affordable Method to Install Fur-Down Interior Ducts

    SciTech Connect

    Beal, D.; McIlvaine , J.; Fonorow, K.; Martin, E.

    2011-11-01

    This document illustrates guidelines for the efficient installation of interior duct systems in new housing, including the fur-up chase method, the fur-down chase method, and interior ducts positioned in sealed attics or sealed crawl spaces. This document illustrates guidelines for the efficient installation of interior duct systems in new housing. Interior ducts result from bringing the duct work inside a home's thermal and air barrier. Architects, designers, builders, and new home buyers should thoroughly investigate any opportunity for energy savings that is as easy to implement during construction, such as the opportunity to construct interior duct work. In addition to enhanced energy efficiency, interior ductwork results in other important advantages, such as improved indoor air quality, increased system durability and increased homeowner comfort. While the advantages of well-designed and constructed interior duct systems are recognized, the implementation of this approach has not gained a significant market acceptance. This guideline describes a variety of methods to create interior ducts including the fur-up chase method, the fur-down chase method, and interior ducts positioned in sealed attics or sealed crawl spaces. As communication of the intent of an interior duct system, and collaboration on its construction are paramount to success, this guideline details the critical design, planning, construction, inspection, and verification steps that must be taken. Involved in this process are individuals from the design team; sales/marketing team; and mechanical, insulation, plumbing, electrical, framing, drywall and solar contractors.

  4. 16 CFR 301.32 - Fur product containing material other than fur.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... required under this Act; as for example: 100% Wool Interlining—100% Recycled Wool Trim—Dyed Muskrat Fur Origin: Canada or Body: 100% Cotton Lining: 100% Nylon Collar: Dyed Mouton Lamb Fur Origin: Argentina (b... required under the Act and rules and regulations; as for example: Body—Leather Trim—Dyed Mink...

  5. 16 CFR 301.32 - Fur product containing material other than fur.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... required under this Act; as for example: 100% Wool Interlining—100% Recycled Wool Trim—Dyed Muskrat Fur Origin: Canada or Body: 100% Cotton Lining: 100% Nylon Collar: Dyed Mouton Lamb Fur Origin: Argentina (b... required under the Act and rules and regulations; as for example: Body—Leather Trim—Dyed Mink...

  6. 16 CFR 301.32 - Fur product containing material other than fur.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... required under this Act; as for example: 100% Wool Interlining—100% Recycled Wool Trim—Dyed Muskrat Fur Origin: Canada or Body: 100% Cotton Lining: 100% Nylon Collar: Dyed Mouton Lamb Fur Origin: Argentina (b... required under the Act and rules and regulations; as for example: Body—Leather Trim—Dyed Mink...

  7. 16 CFR 301.32 - Fur product containing material other than fur.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... required under this Act; as for example: 100% Wool Interlining—100% Recycled Wool Trim—Dyed Muskrat Fur Origin: Canada or Body: 100% Cotton Lining: 100% Nylon Collar: Dyed Mouton Lamb Fur Origin: Argentina (b... required under the Act and rules and regulations; as for example: Body—Leather Trim—Dyed Mink...

  8. 16 CFR 301.32 - Fur product containing material other than fur.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... required under this Act; as for example: 100% Wool Interlining—100% Recycled Wool Trim—Dyed Muskrat Fur Origin: Canada or Body: 100% Cotton Lining: 100% Nylon Collar: Dyed Mouton Lamb Fur Origin: Argentina (b... required under the Act and rules and regulations; as for example: Body—Leather Trim—Dyed Mink...

  9. Seal arrangement

    DOEpatents

    Lundholm, Gunnar

    1987-01-01

    A seal arrangement is provided for preventing gas leakage along a reciprocating piston rod or other reciprocating member passing through a wall which separates a high pressure gas chmber and a low pressure gas chamber. Liquid lubricant is applied to the lower pressure side of a sealing gland surrounding the piston rod to prevent the escape of gas between the rod and the gland. The sealing gland is radially forced against the piston rod by action of a plurality of axially stacked O-rings influenced by an axially acting spring as well as pressure from the gas.

  10. 16 CFR 301.12 - Country of origin of imported furs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... labeling shall be preceded by the term fur origin; as for example: Dyed Muskrat Fur Origin: Russia or Dyed... example: Tip-dyed Canadian American Sable Fur Origin: Canada or Russian Sable Fur Origin: Russia...

  11. The fur transcription regulator and fur-regulated genes in Clostridium botulinum A ATCC 3502.

    PubMed

    Zhang, Weibin; Ma, Junhua; Zang, Chengyuan; Song, Yingying; Liu, Peipei

    2011-01-01

    Clostridium botulinum is a spore-forming bacterium that can produce a very powerful neurotoxin that causes botulism. In this study, we have investigated the Fur transcription regulators in Clostridium botulinum and Fur-regulated genes in Clostridium botulinum A ATCC 3502. We found that gene loss may be the main cause leading to the different numbers of Fur transcription regulators in different Clostridium botulinum strains. Meanwhile, 46 operons were found to be regulated by the Fur transcription regulator in Clostridium botulinum A ATCC 3502, involved in several functional classifications, including iron acquisition, iron utilization, iron transport, and transcription regulator. Under an iron-restricted medium, we experimentally found that a Fur transcription regulator (CBO1372) and two operons (DedA, CBO2610-CBO2614 and ABC transporter, CBO0845-CBO0847) are shown to be differentially expressed in Clostridium botulinum A ATCC 3502. This study has provided-us novel insights into the diversity of Fur transcription regulators in different Clostridium botulinum strains and diversity of Fur-targeted genes, as well as a better understanding of the dynamic changes in iron restriction occurring in response to this stress.

  12. 77 FR 57043 - Regulations Under the Fur Products Labeling Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ...The Federal Trade Commission proposes to amend its Regulations under the Fur Products Labeling Act to update its Fur Products Name Guide, provide more labeling flexibility, incorporate recently enacted Truth in Fur Labeling Act provisions, and eliminate unnecessary requirements. The Commission does not propose changing or providing alternatives to the required name on labels for nyctereutes......

  13. 16 CFR 301.17 - Misrepresentation of origin of furs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Misrepresentation of origin of furs. 301.17... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.17 Misrepresentation of origin of furs. No misleading nor deceptive statements as to the geographical or zoological origin of...

  14. 16 CFR 301.17 - Misrepresentation of origin of furs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Misrepresentation of origin of furs. 301.17... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.17 Misrepresentation of origin of furs. No misleading nor deceptive statements as to the geographical or zoological origin of...

  15. 16 CFR 301.17 - Misrepresentation of origin of furs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Misrepresentation of origin of furs. 301.17... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.17 Misrepresentation of origin of furs. No misleading nor deceptive statements as to the geographical or zoological origin of...

  16. 16 CFR 301.17 - Misrepresentation of origin of furs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Misrepresentation of origin of furs. 301.17... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.17 Misrepresentation of origin of furs. No misleading nor deceptive statements as to the geographical or zoological origin of...

  17. 16 CFR 301.17 - Misrepresentation of origin of furs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Misrepresentation of origin of furs. 301.17... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.17 Misrepresentation of origin of furs. No misleading nor deceptive statements as to the geographical or zoological origin of...

  18. 16 CFR 301.23 - Second-hand fur products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Second-hand fur products. 301.23 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.23 Second-hand fur products. When a...-hand”, “Reconditioned-Second-hand”, or “Rebuilt-Second-hand”, as the case may be....

  19. 16 CFR 301.23 - Second-hand fur products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Second-hand fur products. 301.23 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.23 Second-hand fur products. When a...-hand”, “Reconditioned-Second-hand”, or “Rebuilt-Second-hand”, as the case may be....

  20. 16 CFR 301.23 - Second-hand fur products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Second-hand fur products. 301.23 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.23 Second-hand fur products. When a...-hand”, “Reconditioned-Second-hand”, or “Rebuilt-Second-hand”, as the case may be....

  1. 16 CFR 301.23 - Second-hand fur products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Second-hand fur products. 301.23 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.23 Second-hand fur products. When a...-hand”, “Reconditioned-Second-hand”, or “Rebuilt-Second-hand”, as the case may be....

  2. 16 CFR 301.23 - Second-hand fur products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Second-hand fur products. 301.23 Section 301... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.23 Second-hand fur products. When a...-hand”, “Reconditioned-Second-hand”, or “Rebuilt-Second-hand”, as the case may be....

  3. 16 CFR 301.38 - Advertising of furs and fur products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... advertisement to a color of the fur which was caused by dyeing, bleaching or other artificial coloring, such... Dyed Persian Lamb Since 1900 or X Company Manufacturers of Fine Muskrat Coats, Capes and Stoles...

  4. The German Interlinguistics Society Gesellschaft fur Interlinguistik.

    ERIC Educational Resources Information Center

    O Riain, Sean

    2003-01-01

    Describes the German interlinguistics society Gesellschaft fur Interlinguistik (GIL), which was founded to bring together interlinguistics and esperantology scholars. Highlights GIL's principal fields of activity and discusses its role in the fields of international linguistic communication, language planning, esperantolgy, and the teaching of…

  5. Fur-mediated global regulatory circuits in pathogenic Neisseria species.

    PubMed

    Yu, Chunxiao; Genco, Caroline Attardo

    2012-12-01

    The ferric uptake regulator (Fur) protein has been shown to function as a repressor of transcription in a number of diverse microorganisms. However, recent studies have established that Fur can function at a global level as both an activator and a repressor of transcription through both direct and indirect mechanisms. Fur-mediated indirect activation occurs via the repression of additional repressor proteins, or small regulatory RNAs, thereby activating transcription of a previously silent gene. Fur mediates direct activation through binding of Fur to the promoter regions of genes. Whereas the repressive mechanism of Fur has been thoroughly investigated, emerging studies on direct and indirect Fur-mediated activation mechanisms have revealed novel global regulatory circuits.

  6. Forming a seal between planar sealing surfaces

    SciTech Connect

    Ezekoye, L.I.; Rusnica, E.J.; Sepp, H.A. Jr.

    1987-12-29

    A method of forming a seal between the confronting planar sealing surfaces on two annular structural members which are drawn together by axially extending bolts is described comprising the steps of: forming a seal assembly by locking a toroidal, crushable seal member within a substantially flat annular spacer member against an annular shoulder formed on the inner diameter thereof with an annular resilient member seated in a radially extending groove in the inner surface of the substantially flat annular member, the flat annular member being axially thinner than the toroidal, crushable seal member; placing the sealing assembly between the planar sealing surfaces and positively aligning the assembly relative to the axially extending bolts; and tightening the bolts to draw the planar sealing surfaces toward each other and into contact with the flat annular member while crushing the toroidal, crushable seal member to form a seal between the planar sealing surfaces.

  7. Python fiber optic seal

    SciTech Connect

    Ystesund, K.; Bartberger, J.; Brusseau, C.; Fleming, P.; Insch, K.; Tolk, K.

    1993-08-01

    Sandia National Laboratories has developed a high security fiber optic seal that incorporates tamper resistance features that are not available in commercial fiber optic seals. The Python Seal is a passive fiber optic loop seal designed to give indication of unauthorized entry. The seal includes a fingerprint feature that provides seal identity information in addition to the unique fiber optic pattern created when the seal is installed. The fiber optic cable used for the seal loop is produced with tamper resistant features that increase the difficulty of attacking that component of a seal. A Seal Reader has been developed that will record the seal signature and the fingerprint feature of the seal. A Correlator software program then compares seal images to establish a match or mismatch. SNL is also developing a Polaroid reader to permit hard copies of the seal patterns to be obtained directly from the seal.

  8. GAS SEAL

    DOEpatents

    Monson, H.; Hutter, E.

    1961-07-11

    A seal is described for a cover closing an opening in the top of a pressure vessel that may house a nuclear reactor. The seal comprises a U-shaped trough formed on the pressure vessel around the opening therein, a mass of metal in the trough, and an edge flange on the cover extending loosely into the trough and dipping into the metal mass. The lower portion of the metal mass is kept melted, and the upper portion, solid. The solid pontion of the metal mass prevents pressure surges in the vessel from expelling the liquid portion of the metal mass from the trough; the liquld portion, thus held in place by the solid portion, does not allow gas to go through, and so gas cannot escape through shrinkage holes in the solid portion.

  9. 16 CFR 301.12 - Country of origin of imported furs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... labeling shall be preceded by the term fur origin; as for example: Dyed Muskrat Fur Origin: Russia or Dyed China Mink Fur Origin: China (3) In addition to the required disclosure of country of origin the name of... example: Tip-dyed Canadian American Sable Fur Origin: Canada or Russian Sable Fur Origin: Russia...

  10. 29 CFR 780.124 - Raising of fur-bearing animals.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur...

  11. 29 CFR 780.124 - Raising of fur-bearing animals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur...

  12. 29 CFR 780.124 - Raising of fur-bearing animals.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 3 2012-07-01 2012-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur...

  13. 29 CFR 780.124 - Raising of fur-bearing animals.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 3 2013-07-01 2013-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur...

  14. 29 CFR 780.124 - Raising of fur-bearing animals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur...

  15. Fluid pressure balanced seal

    NASA Technical Reports Server (NTRS)

    Marsh, H. W. (Inventor)

    1966-01-01

    A seal which increases in effectiveness with increasing pressure is presented. The seal's functional capability throughout both static and dynamic operation makes it particularly useful for sealing ball valve ports. Other features of the seal include the ability to seal two opposed surfaces simultaneously, tolerance of small misalignments, tolerance of wide temperature ranges, ability to maintain positive sealing contact under conditions of internal or external pressurization, and ability to conform to slight irregularities in seal or surface contours.

  16. Turbine with radial acting seal

    SciTech Connect

    Eng, Darryl S; Ebert, Todd A

    2016-11-22

    A floating brush seal in a rim cavity of a turbine in a gas turbine engine, where the floating brush seal includes a seal holder in which the floating brush seal floats, and a expandable seal that fits within two radial extending seal slots that maintains a seal with radial displacement of the floating brush seal and the seal holder.

  17. Dynamic Face Seal Arrangement

    NASA Technical Reports Server (NTRS)

    Dellacorte, Christopher (Inventor)

    1999-01-01

    A radial face seal arrangement is disclosed comprising a stationary seal ring that is spring loaded against a seal seat affixed to a rotating shaft. The radial face seal arrangement further comprises an arrangement that not only allows for preloading of the stationary seal ring relative to the seal seat, but also provides for dampening yielding a dynamic seating response for the radial face seal arrangement. The overall seal system, especially regarding the selection of the material for the stationary seal ring, is designed to operate over a wide temperature range from below ambient up to 900 C.

  18. Regenerator seal design

    DOEpatents

    Eckart, Francis H.

    1982-01-01

    A rotary regenerator disc matrix has a face seal with a cross arm and arcuate rim segments joined by prestress clamps to prestrain the arcuate rim seals so as to compensate seal rim twisting or coning and resultant disc face seal leakage as produced by operating thermal gradients across the seal.

  19. Sealing arrangement

    SciTech Connect

    Brandenstein, M.; Hans, R.; Kiener, H.

    1985-04-09

    An hydraulically operated clutch release has an annular piston hydraulically moveable in an annular chamber. A felt ring is mounted on a shoulder of the piston away from the chamber, to engage the bore surface of the chamber, and a holding ring is snapped onto the end of the shoulder to hold the felt ring on the shoulder. An extension of the holding ring forms a seal with the bore on the chamber, and defines, with the felt ring a dust catching recesS.

  20. 50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? 23.69... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? (a) U.S. and... Article II(2)(b) of the Treaty (see § 23.89). The import, export, or re-export of fur skins and fur...

  1. Regenerator seal

    DOEpatents

    Davis, Leonard C.; Pacala, Theodore; Sippel, George R.

    1981-01-01

    A method for manufacturing a hot side regenerator cross arm seal assembly having a thermally stablilized wear coating with a substantially flat wear surface thereon to seal between low pressure and high pressure passages to and from the hot inboard side of a rotary regenerator matrix includes the steps of forming a flat cross arm substrate member of high nickel alloy steel; fixedly securing the side edges of the substrate member to a holding fixture with a concave surface thereacross to maintain the substrate member to a slightly bent configuration on the fixture surface between the opposite ends of the substrate member to produce prestress therein; applying coating layers on the substrate member including a wear coating of plasma sprayed nickel oxide/calcium flouride material to define a wear surface of slightly concave form across the restrained substrate member between the free ends thereon; and thereafter subjecting the substrate member and the coating thereon to a heat treatment of 1600.degree. F. for sixteen hours to produce heat stabilizing growth in the coating layers on the substrate member and to produce a thermally induced growth stress in the wear surface that substantially equalizes the prestress in the substrate whereby when the cross arm is removed from the fixture surface following the heat treatment step a wear face is formed on the cross arm assembly that will be substantially flat between the ends.

  2. Damper Spring For Omega Seal

    NASA Technical Reports Server (NTRS)

    Maclaughlin, Scott T.; Montgomery, Stuart K.

    1993-01-01

    Damper spring reduces deflections of omega-cross-section seal, reducing probability of failure and extending life of seal. Spring is split ring with U-shaped cross section. Placed inside omega seal and inserted with seal into seal cavity. As omega seal compressed into cavity, spring and seal make contact near convolution of seal, and spring becomes compressed also. During operation, when seal dynamically loaded, spring limits deflection of seal, reducing stress on seal.

  3. Triple acting radial seal

    SciTech Connect

    Ebert, Todd A; Carella, John A

    2012-03-13

    A triple acting radial seal used as an interstage seal assembly in a gas turbine engine, where the seal assembly includes an interstage seal support extending from a stationary inner shroud of a vane ring, the interstage seal support includes a larger annular radial inward facing groove in which an outer annular floating seal assembly is secured for radial displacement, and the outer annular floating seal assembly includes a smaller annular radial inward facing groove in which an inner annular floating seal assembly is secured also for radial displacement. A compliant seal is secured to the inner annular floating seal assembly. The outer annular floating seal assembly encapsulates the inner annular floating seal assembly which is made from a very low alpha material in order to reduce thermal stress.

  4. Expression of fur and its antisense α-fur from Microcystis aeruginosa PCC7806 as response to light and oxidative stress.

    PubMed

    Martin-Luna, Beatriz; Sevilla, Emma; Gonzalez, Andres; Bes, M Teresa; Fillat, Maria F; Peleato, M Luisa

    2011-12-15

    Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate signaling of iron metabolism and oxidative stress responses with several environmental stresses. In photosynthetic organisms, Fur proteins regulate many genes involved in photosynthesis, nitrogen metabolism and other key processes. Also, Fur triggers the expression of virulence factors in many bacterial pathogens, and Fur from Microcystis aeruginosa has been shown to bind promoter regions of the microcystin synthesis gene cluster. In this work, we studied transcriptional responses of fur genes under different light intensities and oxidative stress. An antisense of fur, the α-fur RNA, plays an important role in regulating fur expression under oxidative stress, affecting levels of Fur protein in cells. Importantly, an active photosynthetic electron chain is required for the expression of the fur gene.

  5. Fundamentals of fluid sealing

    NASA Technical Reports Server (NTRS)

    Zuk, J.

    1976-01-01

    The fundamentals of fluid sealing, including seal operating regimes, are discussed and the general fluid-flow equations for fluid sealing are developed. Seal performance parameters such as leakage and power loss are presented. Included in the discussion are the effects of geometry, surface deformations, rotation, and both laminar and turbulent flows. The concept of pressure balancing is presented, as are differences between liquid and gas sealing. Mechanisms of seal surface separation, fundamental friction and wear concepts applicable to seals, seal materials, and pressure-velocity (PV) criteria are discussed.

  6. Using Fur to Estimate Mercury Concentrations in Mink

    EPA Science Inventory

    Total mercury (Hg) concentrations in fur and muscle tissue from mink (Mustela vison) were compared to determine the utility of fur analysis as a non-lethal and convenient method for predicting mercury concentrations in tissues. Sixty nine wild-trapped mink were collected in Rhode...

  7. 50 CFR 31.17 - Disposal of furs and pelts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false Disposal of furs and pelts. 31.17 Section 31.17 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... Reduction and Disposal § 31.17 Disposal of furs and pelts. The disposition of animals and the pelts...

  8. 50 CFR 31.17 - Disposal of furs and pelts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 9 2014-10-01 2014-10-01 false Disposal of furs and pelts. 31.17 Section 31.17 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... Reduction and Disposal § 31.17 Disposal of furs and pelts. The disposition of animals and the pelts...

  9. 50 CFR 31.17 - Disposal of furs and pelts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 9 2012-10-01 2012-10-01 false Disposal of furs and pelts. 31.17 Section 31.17 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... Reduction and Disposal § 31.17 Disposal of furs and pelts. The disposition of animals and the pelts...

  10. 50 CFR 31.17 - Disposal of furs and pelts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 9 2013-10-01 2013-10-01 false Disposal of furs and pelts. 31.17 Section 31.17 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... Reduction and Disposal § 31.17 Disposal of furs and pelts. The disposition of animals and the pelts...

  11. Turbine disc sealing assembly

    DOEpatents

    Diakunchak, Ihor S.

    2013-03-05

    A disc seal assembly for use in a turbine engine. The disc seal assembly includes a plurality of outwardly extending sealing flange members that define a plurality of fluid pockets. The sealing flange members define a labyrinth flow path therebetween to limit leakage between a hot gas path and a disc cavity in the turbine engine.

  12. Transcriptional regulation by Ferric Uptake Regulator (Fur) in pathogenic bacteria.

    PubMed

    Troxell, Bryan; Hassan, Hosni M

    2013-01-01

    In the ancient anaerobic environment, ferrous iron (Fe(2+)) was one of the first metal cofactors. Oxygenation of the ancient world challenged bacteria to acquire the insoluble ferric iron (Fe(3+)) and later to defend against reactive oxygen species (ROS) generated by the Fenton chemistry. To acquire Fe(3+), bacteria produce low-molecular weight compounds, known as siderophores, which have extremely high affinity for Fe(3+). However, during infection the host restricts iron from pathogens by producing iron- and siderophore-chelating proteins, by exporting iron from intracellular pathogen-containing compartments, and by limiting absorption of dietary iron. Ferric Uptake Regulator (Fur) is a transcription factor which utilizes Fe(2+) as a corepressor and represses siderophore synthesis in pathogens. Fur, directly or indirectly, controls expression of enzymes that protect against ROS damage. Thus, the challenges of iron homeostasis and defense against ROS are addressed via Fur. Although the role of Fur as a repressor is well-documented, emerging evidence demonstrates that Fur can function as an activator. Fur activation can occur through three distinct mechanisms (1) indirectly via small RNAs, (2) binding at cis regulatory elements that enhance recruitment of the RNA polymerase holoenzyme (RNAP), and (3) functioning as an antirepressor by removing or blocking DNA binding of a repressor of transcription. In addition, Fur homologs control defense against peroxide stress (PerR) and control uptake of other metals such as zinc (Zur) and manganese (Mur) in pathogenic bacteria. Fur family members are important for virulence within bacterial pathogens since mutants of fur, perR, or zur exhibit reduced virulence within numerous animal and plant models of infection. This review focuses on the breadth of Fur regulation in pathogenic bacteria.

  13. Mechanical seal assembly

    DOEpatents

    Kotlyar, Oleg M.

    2001-01-01

    An improved mechanical seal assembly is provided for sealing rotating shafts with respect to their shaft housings, wherein the rotating shafts are subject to substantial axial vibrations. The mechanical seal assembly generally includes a rotating sealing ring fixed to the shaft, a non-rotating sealing ring adjacent to and in close contact with the rotating sealing ring for forming an annular seal about the shaft, and a mechanical diode element that applies a biasing force to the non-rotating sealing ring by means of hemispherical joint. The alignment of the mechanical diode with respect to the sealing rings is maintained by a series of linear bearings positioned axially along a desired length of the mechanical diode. Alternative embodiments include mechanical or hydraulic amplification components for amplifying axial displacement of the non-rotating sealing ring and transferring it to the mechanical diode.

  14. Mechanical seal assembly

    DOEpatents

    Kotlyar, Oleg M.

    2002-01-01

    An improved mechanical seal assembly is provided for sealing rotating shafts with respect to their shaft housings, wherein the rotating shafts are subject to substantial axial vibrations. The mechanical seal assembly generally includes a rotating sealing ring fixed to the shaft, a non-rotating sealing ring adjacent to and in close contact with the rotating sealing ring for forming an annular seal about the shaft, and a mechanical diode element that applies a biasing force to the non-rotating sealing ring by means of hemispherical joint. The alignment of the mechanical diode with respect to the sealing rings is maintained by a series of linear bearings positioned axially along a desired length of the mechanical diode. Alternative embodiments include mechanical or hydraulic amplification components for amplifying axial displacement of the non-rotating sealing ring and transfering it to the mechanical diode.

  15. Turbine blade platform seal

    SciTech Connect

    Zagar, Thomas W.; Schiavo, Anthony L.

    2001-01-01

    A rotating blade group 90 for a turbo-machine having an improved device for sealing the gap 110 between the edges 112,114 of adjacent blade platforms 96,104. The gap 110 between adjacent blades 92,100 is sealed by a seal pin 20 its central portion 110 and by a seal plate 58,60 at each of the front 54 and rear 56 portions. The seal plates 58,60 are inserted into corresponding grooves 62,64 formed in the adjacent edges 112,114 of adjoining blades 92,100 and held in place by end plates 40,42. The end of the seal plates 58,60 may be chamfered 78,80 to improve the seal against the end plate 40,42. The seal pin 20 provides the required damping between the blades 92,100 and the seal plates 58,60 provide improved sealing effectiveness.

  16. Seal design alternatives study

    SciTech Connect

    Van Sambeek, L.L.

    1993-06-01

    This report presents the results from a study of various sealing alternatives for the WIPP sealing system. Overall, the sealing system has the purpose of reducing to the extent possible the potential for fluids (either gas or liquid) from entering or leaving the repository. The sealing system is divided into three subsystems: drift and panel seals within the repository horizon, shaft seals in each of the four shafts, and borehole seals. Alternatives to the baseline configuration for the WIPP seal system design included evaluating different geometries and schedules for seal component installations and the use of different materials for seal components. Order-of-magnitude costs for the various alternatives were prepared as part of the study. Firm recommendations are not presented, but the advantages and disadvantages of the alternatives are discussed. Technical information deficiencies are identified and studies are outlined which can provide required information.

  17. Characterization of the Neisseria gonorrhoeae Iron and Fur Regulatory Network

    PubMed Central

    Yu, Chunxiao; McClure, Ryan; Daou, Nadine

    2016-01-01

    ABSTRACT The Neisseria gonorrhoeae ferric uptake regulator (Fur) protein controls expression of iron homeostasis genes in response to intracellular iron levels. In this study, using transcriptome sequencing (RNA-seq) analysis of an N. gonorrhoeae fur strain, we defined the gonococcal Fur and iron regulons and characterized Fur-controlled expression of an ArsR-like DNA binding protein. We observed that 158 genes (8% of the genome) showed differential expression in response to iron in an N. gonorrhoeae wild-type or fur strain, while 54 genes exhibited differential expression in response to Fur. The Fur regulon was extended to additional regulators, including NrrF and 13 other small RNAs (sRNAs), and two transcriptional factors. One transcriptional factor, coding for an ArsR-like regulator (ArsR), exhibited increased expression under iron-replete conditions in the wild-type strain but showed decreased expression across iron conditions in the fur strain, an effect that was reversed in a fur-complemented strain. Fur was shown to bind to the promoter region of the arsR gene downstream of a predicted σ70 promoter region. Electrophoretic mobility shift assay (EMSA) analysis confirmed binding of the ArsR protein to the norB promoter region, and sequence analysis identified two additional putative targets, NGO1411 and NGO1646. A gonococcal arsR strain demonstrated decreased survival in human endocervical epithelial cells compared to that of the wild-type and arsR-complemented strains, suggesting that the ArsR regulon includes genes required for survival in host cells. Collectively, these results demonstrate that the N. gonorrhoeae Fur functions as a global regulatory protein to repress or activate expression of a large repertoire of genes, including additional transcriptional regulatory proteins. IMPORTANCE Gene regulation in bacteria in response to environmental stimuli, including iron, is of paramount importance to both bacterial replication and, in the case of pathogenic

  18. Keeping warm with fur in cold water: entrainment of air in hairy surfaces

    NASA Astrophysics Data System (ADS)

    Nasto, Alice; Regli, Marianne; Brun, Pierre-Thomas; Clanet, Christophe; Hosoi, Anette

    2015-11-01

    Instead of relying on a thick layer of body fat for insulation as many aquatic mammals do, fur seals and otters trap air in their dense fur for insulation in cold water. Using a combination of model experiments and theory, we rationalize this mechanism of air trapping underwater for thermoregulation. For the model experiments, hairy surfaces are fabricated using laser cut molds and casting samples with PDMS. Modeling the hairy texture as a network of capillary tubes, the imbibition speed of water into the hairs is obtained through a balance of hydrostatic pressure and viscous stress. In this scenario, the bending of the hairs and capillary forces are negligible. The maximum diving depth that can be achieved before the hairs are wetted to the roots is predicted from a comparison of the diving speed and imbibition speed. The amount of air that is entrained in hairy surfaces is greater than what is expected for classic Landau-Levich-Derjaguin plate plunging. A phase diagram with the parameters from experiments and biological data allows a comparison of the model system and animals.

  19. Rotary shaft sealing assembly

    DOEpatents

    Dietle, Lannie L; Schroeder, John E; Kalsi, Manmohan S; Alvarez, Patricio D

    2013-08-13

    A rotary shaft sealing assembly in which a first fluid is partitioned from a second fluid in a housing assembly having a rotary shaft located at least partially within. In one embodiment a lip seal is lubricated and flushed with a pressure-generating seal ring preferably having an angled diverting feature. The pressure-generating seal ring and a hydrodynamic seal may be used to define a lubricant-filled region with each of the seals having hydrodynamic inlets facing the lubricant-filled region. Another aspect of the sealing assembly is having a seal to contain pressurized lubricant while withstanding high rotary speeds. Another rotary shaft sealing assembly embodiment includes a lubricant supply providing a lubricant at an elevated pressure to a region between a lip seal and a hydrodynamic seal with a flow control regulating the flow of lubricant past the lip seal. The hydrodynamic seal may include an energizer element having a modulus of elasticity greater than the modulus of elasticity of a sealing lip of the hydrodynamic seal.

  20. Rotary shaft sealing assembly

    DOEpatents

    Dietle, Lannie L.; Schroeder, John E.; Kalsi, Manmohan S.; Alvarez, Patricio D.

    2010-09-21

    A rotary shaft sealing assembly in which a first fluid is partitioned from a second fluid in a housing assembly having a rotary shaft located at least partially within. In one embodiment a lip seal is lubricated and flushed with a pressure-generating seal ring preferably having an angled diverting feature. The pressure-generating seal ring and a hydrodynamic seal may be used to define a lubricant-filled region with each of the seals having hydrodynamic inlets facing the lubricant-filled region. Another aspect of the sealing assembly is having a seal to contain pressurized lubricant while withstanding high rotary speeds. Another rotary shaft sealing assembly embodiment includes a lubricant supply providing a lubricant at an elevated pressure to a region between a lip seal and a hydrodynamic seal with a flow control regulating the flow of lubricant past the lip seal. The hydrodynamic seal may include an energizer element having a modulus of elasticity greater than the modulus of elasticity of a sealing lip of the hydrodynamic seal.

  1. The modified Cobra Seal

    SciTech Connect

    Ystesund, K.J.; Drayer, D.D.

    1990-01-01

    The Cobra Seal was developed in response to the International Atomic Energy Agency's request for an in situ verifiable seal. The Type E metal cap seal, still widely used by the IAEA, must be removed and returned to Agency headquarters for verification. The Cobra Seal allows an inspector to verify seal identity and integrity on site, without removing the seal. The seal consists of a loop of multi-strand fiber optic cable, which can be routed around or through the object to be sealed, and a seal body that secures the ends of the fiber optic cable. A cutting blade in the seal body randomly cuts a portion of the optical fibers in the cable. After the seal assembly is completed, a reference image is recorded of the unique pattern of light spots produced when the seal face is illuminated. Subsequent photographs of the seal pattern are compared to the original to establish the seal identity and integrity. This paper reviews the improvements and the technology of the cobra seal system. 3 refs., 5 figs.

  2. 16 CFR 301.14 - Country of origin of used furs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Country of origin of used furs. 301.14... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.14 Country of origin of used furs. When the country of origin of used furs is unknown, and no representations are made directly...

  3. 16 CFR 301.34 - Misbranded or falsely invoiced fur products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Misbranded or falsely invoiced fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.34 Misbranded or falsely invoiced fur products. (a) If a person subject to section 3 of the Act with respect to a fur product...

  4. Fur-mediated activation of gene transcription in the human pathogen Neisseria gonorrhoeae.

    PubMed

    Yu, Chunxiao; Genco, Caroline Attardo

    2012-04-01

    It is well established that the ferric uptake regulatory protein (Fur) functions as a transcriptional repressor in diverse microorganisms. Recent studies demonstrated that Fur also functions as a transcriptional activator. In this study we defined Fur-mediated activation of gene transcription in the sexually transmitted disease pathogen Neisseria gonorrhoeae. Analysis of 37 genes which were previously determined to be iron induced and which contained putative Fur boxes revealed that only 30 of these genes exhibited reduced transcription in a gonococcal fur mutant strain. Fur-mediated activation was established by examining binding of Fur to the putative promoter regions of 16 Fur-activated genes with variable binding affinities observed. Only ∼50% of the newly identified Fur-regulated genes bound Fur in vitro, suggesting that additional regulatory circuits exist which may function through a Fur-mediated indirect mechanism. The gonococcal Fur-activated genes displayed variable transcription patterns in a fur mutant strain, which correlated with the position of the Fur box in each (promoter) region. These results suggest that Fur-mediated direct transcriptional activation is fulfilled by multiple mechanisms involving either competing with a repressor or recruiting RNA polymerase. Collectively, our studies have established that gonococcal Fur functions as an activator of gene transcription through both direct and indirect mechanisms.

  5. 16 CFR 301.24 - Repairing, restyling and remodeling fur products for consumer.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Repairing, restyling and remodeling fur... UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.24 Repairing, restyling and remodeling fur products for consumer. When fur products owned by and...

  6. 16 CFR 301.14 - Country of origin of used furs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Country of origin of used furs. 301.14... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.14 Country of origin of used furs. When the country of origin of used furs is unknown, and no representations are made directly...

  7. 16 CFR 301.5 - Use of Fur Products Name Guide.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Use of Fur Products Name Guide. 301.5... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.5 Use of Fur Products Name Guide. (a) The Fur Products Name Guide (§ 301.0 of this part) is set up in four columns under the...

  8. 16 CFR 301.34 - Misbranded or falsely invoiced fur products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Misbranded or falsely invoiced fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.34 Misbranded or falsely invoiced fur products. (a) If a person subject to section 3 of the Act with respect to a fur product...

  9. 16 CFR 301.6 - Animals not listed in Fur Products Name Guide.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Animals not listed in Fur Products Name... OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.6 Animals not listed in Fur Products Name Guide. (a) All furs are subject to the act and regulations regardless...

  10. 16 CFR 301.34 - Misbranded or falsely invoiced fur products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Misbranded or falsely invoiced fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.34 Misbranded or falsely invoiced fur products. (a) If a person subject to section 3 of the Act with respect to a fur product...

  11. 16 CFR 301.5 - Use of Fur Products Name Guide.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Use of Fur Products Name Guide. 301.5... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.5 Use of Fur Products Name Guide. (a) The Fur Products Name Guide (§ 301.0 of this part) is set up in four columns under the...

  12. 16 CFR 301.5 - Use of Fur Products Name Guide.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Use of Fur Products Name Guide. 301.5... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.5 Use of Fur Products Name Guide. (a) The Fur Products Name Guide (§ 301.0 of this part) is set up in four columns under the...

  13. 16 CFR 301.6 - Animals not listed in Fur Products Name Guide.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Animals not listed in Fur Products Name... OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.6 Animals not listed in Fur Products Name Guide. (a) All furs are subject to the act and regulations regardless...

  14. 16 CFR 301.24 - Repairing, restyling and remodeling fur products for consumer.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Repairing, restyling and remodeling fur... UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.24 Repairing, restyling and remodeling fur products for consumer. When fur products owned by and...

  15. 16 CFR 301.14 - Country of origin of used furs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Country of origin of used furs. 301.14... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.14 Country of origin of used furs. When the country of origin of used furs is unknown, and no representations are made directly...

  16. 16 CFR 301.6 - Animals not listed in Fur Products Name Guide.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Animals not listed in Fur Products Name... OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.6 Animals not listed in Fur Products Name Guide. (a) All furs are subject to the act and regulations regardless...

  17. 16 CFR 301.15 - Designation of section producing domestic furs permitted.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... furs permitted. 301.15 Section 301.15 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.15 Designation of section producing domestic furs permitted. In the case of furs produced in the United...

  18. 16 CFR 301.5 - Use of Fur Products Name Guide.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Use of Fur Products Name Guide. 301.5... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.5 Use of Fur Products Name Guide. (a) The Fur Products Name Guide (§ 301.0 of this part) is set up in four columns under the...

  19. 16 CFR 301.24 - Repairing, restyling and remodeling fur products for consumer.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Repairing, restyling and remodeling fur... UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.24 Repairing, restyling and remodeling fur products for consumer. When fur products owned by and...

  20. 16 CFR 301.15 - Designation of section producing domestic furs permitted.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... furs permitted. 301.15 Section 301.15 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.15 Designation of section producing domestic furs permitted. In the case of furs produced in the United...

  1. 16 CFR 301.15 - Designation of section producing domestic furs permitted.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... furs permitted. 301.15 Section 301.15 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.15 Designation of section producing domestic furs permitted. In the case of furs produced in the United...

  2. 16 CFR 301.15 - Designation of section producing domestic furs permitted.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... furs permitted. 301.15 Section 301.15 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.15 Designation of section producing domestic furs permitted. In the case of furs produced in the United...

  3. 16 CFR 301.14 - Country of origin of used furs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Country of origin of used furs. 301.14... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.14 Country of origin of used furs. When the country of origin of used furs is unknown, and no representations are made directly...

  4. 16 CFR 301.14 - Country of origin of used furs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Country of origin of used furs. 301.14... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.14 Country of origin of used furs. When the country of origin of used furs is unknown, and no representations are made directly...

  5. 16 CFR 301.34 - Misbranded or falsely invoiced fur products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Misbranded or falsely invoiced fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.34 Misbranded or falsely invoiced fur products. (a) If a person subject to section 3 of the Act with respect to a fur product...

  6. 16 CFR 301.6 - Animals not listed in Fur Products Name Guide.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Animals not listed in Fur Products Name... OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.6 Animals not listed in Fur Products Name Guide. (a) All furs are subject to the act and regulations regardless...

  7. 16 CFR 301.34 - Misbranded or falsely invoiced fur products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Misbranded or falsely invoiced fur products... CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.34 Misbranded or falsely invoiced fur products. (a) If a person subject to section 3 of the Act with respect to a fur product...

  8. 16 CFR 301.15 - Designation of section producing domestic furs permitted.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... furs permitted. 301.15 Section 301.15 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.15 Designation of section producing domestic furs permitted. In the case of furs produced in the United...

  9. 16 CFR 301.6 - Animals not listed in Fur Products Name Guide.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Animals not listed in Fur Products Name... OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.6 Animals not listed in Fur Products Name Guide. (a) All furs are subject to the act and regulations regardless...

  10. 16 CFR 301.5 - Use of Fur Products Name Guide.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Use of Fur Products Name Guide. 301.5... RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.5 Use of Fur Products Name Guide. (a) The Fur Products Name Guide (§ 301.0 of this part) is set up in four columns under the...

  11. 16 CFR 301.24 - Repairing, restyling and remodeling fur products for consumer.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Repairing, restyling and remodeling fur... UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.24 Repairing, restyling and remodeling fur products for consumer. When fur products owned by and...

  12. Candidiasis in captive pinnipeds.

    PubMed

    Dunn, J L; Buck, J D; Spotte, S

    1984-12-01

    Diagnosis, treatment, and possible pathogenesis of candidiasis were studied in 5 species of pinnipeds in captivity: gray seal (Halichoerus grypus), harbor seal (Phoca vitulina), northern fur seal (Callorhinus ursinus), California sea lion (Zalophus californianus), and northern elephant seal (Mirounga angustirostris). The animals were kept outdoors in a freshwater exhibit. Candidiasis was characterized by purulent nasal discharge, inflammation of the lips at the mucocutaneous junction, periocular alopecia, vaginitis, and dermatitis. Administration of ketoconazole at dosages of 5 mg/kg BID and 10 mg/kg SID controlled the disease. Wild gulls were suspected as vectors of Candida albicans.

  13. Environmental Assessment for Airborne Laser Debris Management Vandenberg AFB, California

    DTIC Science & Technology

    2008-07-01

    California stock of the sperm whale (Physeter macrocephalus) has been designated as "strategic." The California sea lion ( Zalophus californianus ...sea lion ( Zalophus californianus ), and northern fur seal (Callorhinus ursinus). These four species breed on land within the Sea Range. Two of...surface after dives lasting 21 to 25 minutes. California Sea Lion ( Zalophus californianus ) The California sea lion does not have a special

  14. Diagnostic Key to the Parasites of Some Marine Mammal

    DTIC Science & Technology

    1980-03-01

    PROGRAM ELEMENT, PROJECT, TASK ARE A -ft WORK-UNIT. NUMBERS 62759N;F52552;ZF525 52001; 510-MMO2 I!, CONTROLLING OFFICE NAME AND ADDRESS...ABSTRACT (Continue on reverse side H necessary and Identity by block number) This is a diagnostic key for identification of the parasites of marine...californianus) Northern fur seal {Callorhinus ursinus) It is written to be used by a veterinary diagnostician as well as a technician in routine

  15. Estimating animal resource selection from telemetry data using point process models

    USGS Publications Warehouse

    Johnson, Devin S.; Hooten, Mevin B.; Kuhn, Carey E.

    2013-01-01

    To demonstrate the analysis of telemetry data with the point process approach, we analysed a data set of telemetry locations from northern fur seals (Callorhinus ursinus) in the Pribilof Islands, Alaska. Both a space–time and an aggregated space-only model were fitted. At the individual level, the space–time analysis showed little selection relative to the habitat covariates. However, at the study area level, the space-only model showed strong selection relative to the covariates.

  16. Myth or relict: Does ancient DNA detect the enigmatic Upland seal?

    PubMed

    Salis, Alexander T; Easton, Luke J; Robertson, Bruce C; Gemmell, Neil; Smith, Ian W G; Weisler, Marshall I; Waters, Jonathan M; Rawlence, Nicolas J

    2016-04-01

    The biological status of the so-called 'Upland seal' has remained contentious ever since historical records described a distinct seal from the uplands of New Zealand's (NZ) remote sub-Antarctic islands. Subsequent genetic surveys of the NZ fur seal (Arctocephalus forsteri) detected two highly-divergent mtDNA clades, hypothesized to represent a post-sealing hybrid swarm between 'mainland' (Australia-NZ; A. forsteri) and sub-Antarctic (putative 'Upland'; A. snaresensis) lineages. We present ancient-DNA analyses of prehistoric mainland NZ and sub-Antarctic fur seals, revealing that both of these genetic lineages were already widely distributed across the region at the time of human arrival. These findings indicate that anthropogenic factors did not contribute to the admixture of these lineages, and cast doubt on the validity of the Upland seal. Human-mediated impacts on Arctocephalus genetic diversity are instead highlighted by a dramatic temporal haplotype frequency-shift due to genetic drift in heavily bottlenecked populations following the cessation of industrial-scale harvesting. These extinction-recolonisation dynamics add to a growing picture of human-mediated change in NZ's coastal and marine ecosystems.

  17. Nuclear reactor sealing system

    DOEpatents

    McEdwards, James A.

    1983-01-01

    A liquid metal-cooled nuclear reactor sealing system. The nuclear reactor includes a vessel sealed at its upper end by a closure head. The closure head comprises at least two components, one of which is rotatable; and the two components define an annulus therebetween. The sealing system includes at least a first and second inflatable seal disposed in series in an upper portion of the annulus. The system further includes a dip seal extending into a body of insulation located adjacent a bottom portion of the closure head. The dip seal comprises a trough formed by a lower portion of one of the components, and a seal blade pendently supported from the other component and extending downwardly into the trough. A body of liquid metal is contained in the trough which submerges a portion of the seal blade. The seal blade is provided with at least one aperture located above the body of liquid metal for providing fluid communication between the annulus intermediate the dip seal and the inflatable seals, and a body of cover gas located inside the vessel. There also is provided means for introducing a purge gas into the annulus intermediate the inflatable seals and the seal blade. The purge gas is introduced in an amount sufficient to substantially reduce diffusion of radioactive cover gas or sodium vapor up to the inflatable seals. The purge gas mixes with the cover gas in the reactor vessel where it can be withdrawn from the vessel for treatment and recycle to the vessel.

  18. Compliant Foil Seal Investigations

    NASA Technical Reports Server (NTRS)

    Proctor, Margaret; Delgado, Irebert

    2004-01-01

    Room temperature testing of an 8.5 inch diameter foil seal was conducted in the High Speed, High Temperature Turbine Seal Test Rig at the NASA Glenn Research Center. The seal was operated at speeds up to 30,000 rpm and pressure differentials up to 75 psid. Seal leakage and power loss data will be presented and compared to brush seal performance. The failure of the seal and rotor coating at 30,000 rpm and 15 psid will be presented and future development needs discussed.

  19. Inboard seal mounting

    DOEpatents

    Hayes, John R.

    1983-01-01

    A regenerator assembly for a gas turbine engine has a hot side seal assembly formed in part by a cast metal engine block having a seal recess formed therein that is configured to supportingly receive ceramic support blocks including an inboard face thereon having a regenerator seal face bonded thereto. A pressurized leaf seal is interposed between the ceramic support block and the cast metal engine block to bias the seal wear face into sealing engagement with a hot side surface of a rotary regenerator matrix.

  20. The structure of the Helicobacter pylori ferric uptake regulator Fur reveals three functional metal binding sites.

    PubMed

    Dian, Cyril; Vitale, Sylvia; Leonard, Gordon A; Bahlawane, Christelle; Fauquant, Caroline; Leduc, Damien; Muller, Cécile; de Reuse, Hilde; Michaud-Soret, Isabelle; Terradot, Laurent

    2011-03-01

    Fur, the ferric uptake regulator, is a transcription factor that controls iron metabolism in bacteria. Binding of ferrous iron to Fur triggers a conformational change that activates the protein for binding to specific DNA sequences named Fur boxes. In Helicobacter pylori, HpFur is involved in acid response and is important for gastric colonization in model animals. Here we present the crystal structure of a functionally active HpFur mutant (HpFur2M; C78S-C150S) bound to zinc. Although its fold is similar to that of other Fur and Fur-like proteins, the crystal structure of HpFur reveals a unique structured N-terminal extension and an unusual C-terminal helix. The structure also shows three metal binding sites: S1 the structural ZnS₄ site previously characterized biochemically in HpFur and the two zinc sites identified in other Fur proteins. Site-directed mutagenesis and spectroscopy analyses of purified wild-type HpFur and various mutants show that the two metal binding sites common to other Fur proteins can be also metallated by cobalt. DNA protection and circular dichroism experiments demonstrate that, while these two sites influence the affinity of HpFur for DNA, only one is absolutely required for DNA binding and could be responsible for the conformational changes of Fur upon metal binding while the other is a secondary site.

  1. Correlations between elements in the fur of wild animals.

    PubMed

    Długaszek, Maria; Kopczyński, Krzysztof

    2014-07-01

    There is little data on the elemental composition of wild animals fur. In the paper, an attempt has been made to evaluate the concentration of elements in the fur of roe deer, wild boar and hare. The contents of following elements: calcium (Ca), magnesium (Mg), zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), cadmium (Cd), aluminium (Al), chromium (Cr), nickel (Ni) were determined by atomic absorption spectrometry method. Their content was in the range 0.01 (Cd) to 1,519 (Ca) μg/g. Correlations between the content of Mn, Al, Ca, Pb, Cr, Ni in the fur of animals, liver and muscle tissues were found. Thus it can be assumed that the fur of wild animals can provide an information on the bioavailability of elements and environmental exposure and can be considered as an useful biomarker in animals and environmental studies, although research on this subject should be continued.

  2. 11. Architect's rendering of the Arcade Building by Furness, Evans ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Architect's rendering of the Arcade Building by Furness, Evans and Co., from Moses King's Philadelphia and Notable Philadelphians, published 1902 for the City's 220th birthday - Arcade Building, Fifteenth & Market Streets, Philadelphia, Philadelphia County, PA

  3. Energy efficient face seal

    NASA Technical Reports Server (NTRS)

    Sehnal, J.; Sedy, J.; Etsion, I.; Zobens, A.

    1982-01-01

    Torque, face temperature, leakage, and wear of a flat face seal were compared with three coned face seals at pressures up to 2758 kPa and speeds up to 8000 rpm. Axial movement of the mating seal parts was recorded by a digital data acquisition system. The coning of the tungsten carbide primary ring ranged from .51 micro-m to 5.6 micro-m. The torque of the coned face seal balanced to 76.3% was an average 42% lower, the leakage eleven times higher, than that of the standard flat face seal. The reduction of the balance of the coned face seal to 51.3% resulted by decreasing the torque by an additional 44% and increasing leakage 12 to 230 times, depending on the seal shaft speed. No measurable wear was observed on the face of the coned seals.

  4. Sealed container sampling device

    NASA Technical Reports Server (NTRS)

    Hennigan, T. J.

    1969-01-01

    Sampling device, by means of a tapered needle, pierces a sealed container while maintaining the seal and either evacuates or pressurizes the container. This device has many applications in the chemical, preservative and battery-manufacturing industries.

  5. Magnetically Actuated Seal

    NASA Technical Reports Server (NTRS)

    Pinera, Alex

    2013-01-01

    This invention is a magnetically actuated seal in which either a single electromagnet, or multiple electromagnets, are used to control the seal's position. This system can either be an open/ close type of system or an actively controlled system.

  6. Titanium sealing glasses and seals formed therefrom

    DOEpatents

    Brow, R.K.; McCollister, H.L.; Phifer, C.C.; Day, D.E.

    1997-12-02

    Alkaline-earth lanthanoborate sealing-glass compositions containing CaO, La{sub 2}O{sub 3}, B{sub 2}O{sub 3}, TiO{sub 2} and Al{sub 2}O{sub 3} in various combinations of mole-% are provided. These sealing-glass compositions are useful for forming hermetic glass-to-metal seals with titanium and titanium alloys that have a high aqueous durability for component or device applications requiring exposure to moisture, water or body fluids. Particular applications of the titanium sealing-glass compositions include forming glass-to-metal seals for lithium batteries and implanted biomedical devices (e.g. batteries, pacemakers, defibrillators, pumps). 2 figs.

  7. Titanium sealing glasses and seals formed therefrom

    DOEpatents

    Brow, Richard K.; McCollister, Howard L.; Phifer, Carol C.; Day, Delbert E.

    1997-01-01

    Alkaline-earth lanthanoborate sealing-glass compositions containing CaO, La.sub.2 O.sub.3, B.sub.2 O.sub.3, TiO.sub.2 and Al.sub.2 O.sub.3 in various combinations of mole-% are provided. These sealing-glass compositions are useful for forming hermetic glass-to-metal seals with titanium and titanium alloys that have a high aqueous durability for component or device applications requiring exposure to moisture, water or body fluids. Particular applications of the titanium sealing-glass compositions include forming glass-to-metal seals for lithium batteries and implanted biomedical devices (e.g. batteries, pacemakers, defibrillators, pumps).

  8. Tamper-indicating seal

    DOEpatents

    Fiarman, Sidney; Degen, Michael F.; Peters, Henry F.

    1985-01-01

    There is disclosed a tamper-indicating seal that permits in the field inspection and detection of tampering. Said seal comprises a shrinkable tube having a visible pattern of markings which is shrunk over the item to be sealed, and a second transparent tube, having a second visible marking pattern, which is shrunk over the item and the first tube. The relationship between the first and second set of markings produces a pattern so that the seal may not be removed without detection.

  9. Security seal. [Patent application

    DOEpatents

    Gobeli, G.W.

    1981-11-17

    Security for a package or verifying seal in plastic material is provided by a print seal with unique thermally produced imprints in the plastic. If tampering is attempted, the material is irreparably damaged and thus detectable. The pattern of the imprints, similar to fingerprints are recorded as a positive identification for the seal, and corresponding recordings made to allow comparison. The integrity of the seal is proved by the comparison of imprint identification records made by laser beam projection.

  10. Turbomachine Interface Sealing

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C.; Chupp, Raymond E.; Lattime, Scott B.; Steinetz, Bruce M.

    2005-01-01

    Sealing interfaces and coatings, like lubricants, are sacrificial, giving up their integrity for the benefit of the component. Clearance control is a major issue in power systems turbomachine design and operational life. Sealing becomes the most cost-effective way to enhance system performance. Coatings, films, and combined use of both metals and ceramics play a major role in maintaining interface clearances in turbomachine sealing and component life. This paper focuses on conventional and innovative materials and design practices for sealing interfaces.

  11. Resilient Braided Rope Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor); Kren, Lawrence A. (Inventor)

    1996-01-01

    A resilient braided rope seal for use in high temperature applications. The resilient braided rope seal includes a center core of fibers, a resilient 5 member overbraided by at least one layer of braided sheath fibers tightly packed together. The resilient member adds significant stiffness to the seal while maintaining resiliency. Furthermore, the seal permanent set and hysteresis are greatly reduced. Finally, improved load capabilities are provided.

  12. 50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear harvested in... trade internationally in fur skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf... of this section, CITES furbearers means bobcat (Lynx rufus), river otter (Lontra canadensis),...

  13. Collapsable seal member

    DOEpatents

    Sherrell, Dennis L.

    1990-01-01

    A hollow, collapsable seal member normally disposed in a natural expanded state offering fail-safe pressure sealing against a seating surface and adapted to be evacuated by a vacuum force for collapsing the seal member to disengage the same from said seating surface.

  14. Skew resisting hydrodynamic seal

    DOEpatents

    Conroy, William T.; Dietle, Lannie L.; Gobeli, Jeffrey D.; Kalsi, Manmohan S.

    2001-01-01

    A novel hydrodynamically lubricated compression type rotary seal that is suitable for lubricant retention and environmental exclusion. Particularly, the seal geometry ensures constraint of a hydrodynamic seal in a manner preventing skew-induced wear and provides adequate room within the seal gland to accommodate thermal expansion. The seal accommodates large as-manufactured variations in the coefficient of thermal expansion of the sealing material, provides a relatively stiff integral spring effect to minimize pressure-induced shuttling of the seal within the gland, and also maintains interfacial contact pressure within the dynamic sealing interface in an optimum range for efficient hydrodynamic lubrication and environment exclusion. The seal geometry also provides for complete support about the circumference of the seal to receive environmental pressure, as compared the interrupted character of seal support set forth in U.S. Pat. Nos. 5,873,576 and 6,036,192 and provides a hydrodynamic seal which is suitable for use with non-Newtonian lubricants.

  15. Seals development and evaluation

    SciTech Connect

    Waddoups, I.G.; Horton, P.R.V.

    1994-08-01

    This paper discusses Sandia`s support of DOE`s domestic seals program. Testing was conducted on several pressure sensitive seals and a few wire loop seals currently in use as well as on a few new seals. The testing on new seals concentrated on loop seals and included two fiber optic seals and a recently available wire loop seal being considered for use. Environmental, handling and vulnerability testing were conducted. The standardized testing approach used and the results of the testing are summarized. The status of evaluations for using higher security active and passive seals for domestic applications is also presented. The conclusion of the testing -of seals currently in use is that, even though there is some variability in their ability to meet all the test criterion, they are all generally acceptable by the test standards used. The motivation for evaluating higher security seals is to ascertain if seals could be used in broader domestic environment and result in improved cost-effectiveness.

  16. Hydraulic System Seal Development.

    DTIC Science & Technology

    1981-06-01

    supplying the seal. Shamban, Fluorocarbon, Parker, Powty, Hercules, Disogrin, Bal Seal Engineering, American Variseal , Tetrafluor, Conover, and Greene...characteristics, as did the Variseal , but both caused minor rod scoring. The Greene Tweed llytrel seal was not considered for further testing becduse

  17. Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli.

    PubMed

    Seo, Sang Woo; Kim, Donghyuk; Latif, Haythem; O'Brien, Edward J; Szubin, Richard; Palsson, Bernhard O

    2014-09-15

    The ferric uptake regulator (Fur) plays a critical role in the transcriptional regulation of iron metabolism. However, the full regulatory potential of Fur remains undefined. Here we comprehensively reconstruct the Fur transcriptional regulatory network in Escherichia coli K-12 MG1655 in response to iron availability using genome-wide measurements. Integrative data analysis reveals that a total of 81 genes in 42 transcription units are directly regulated by three different modes of Fur regulation, including apo- and holo-Fur activation and holo-Fur repression. We show that Fur connects iron transport and utilization enzymes with negative-feedback loop pairs for iron homeostasis. In addition, direct involvement of Fur in the regulation of DNA synthesis, energy metabolism and biofilm development is found. These results show how Fur exhibits a comprehensive regulatory role affecting many fundamental cellular processes linked to iron metabolism in order to coordinate the overall response of E. coli to iron availability.

  18. Functional Analysis of the Ferric Uptake Regulator Gene fur in Xanthomonas vesicatoria.

    PubMed

    Liu, Huiqin; Dong, Chunling; Zhao, Tingchang; Han, Jucai; Wang, Tieling; Wen, Xiangzhen; Huang, Qi

    2016-01-01

    Iron is essential for the growth and survival of many organisms. Intracellular iron homeostasis must be maintained for cell survival and protection against iron toxicity. The ferric uptake regulator protein (Fur) regulates the high-affinity ferric uptake system in many bacteria. To investigate the function of the fur gene in Xanthomonas vesicatoria (Xv), we generated a fur mutant strain, fur-m, by site-directed mutagenesis. Whereas siderophore production increased in the Xv fur mutant, extracellular polysaccharide production, biofilm formation, swimming ability and quorum sensing signals were all significantly decreased. The fur mutant also had significantly reduced virulence in tomato leaves. The above-mentioned phenotypes significantly recovered when the Xv fur mutation allele was complemented with a wild-type fur gene. Thus, Fur either negatively or positively regulates multiple important physiological functions in Xv.

  19. Functional Analysis of the Ferric Uptake Regulator Gene fur in Xanthomonas vesicatoria

    PubMed Central

    Liu, Huiqin; Dong, Chunling; Zhao, Tingchang; Han, Jucai; Wang, Tieling; Wen, Xiangzhen; Huang, Qi

    2016-01-01

    Iron is essential for the growth and survival of many organisms. Intracellular iron homeostasis must be maintained for cell survival and protection against iron toxicity. The ferric uptake regulator protein (Fur) regulates the high-affinity ferric uptake system in many bacteria. To investigate the function of the fur gene in Xanthomonas vesicatoria (Xv), we generated a fur mutant strain, fur-m, by site-directed mutagenesis. Whereas siderophore production increased in the Xv fur mutant, extracellular polysaccharide production, biofilm formation, swimming ability and quorum sensing signals were all significantly decreased. The fur mutant also had significantly reduced virulence in tomato leaves. The above-mentioned phenotypes significantly recovered when the Xv fur mutation allele was complemented with a wild-type fur gene. Thus, Fur either negatively or positively regulates multiple important physiological functions in Xv. PMID:26910324

  20. Vesicular exanthema of swine and San Miguel sea lion virus: experimental and field studies in otarid seals, feeding trials in swine.

    PubMed

    Gelberg, H B; Dieterich, R A; Lewis, R M

    1982-07-01

    The naturally occurring disease caused by San Miguel sea lion virus in fur seals was characterized by small fluid-filled vesicles 1 to 25 mm in diameter on the nonhaired portions of the flippers. Early epithelial lesions contained multifocal sites of cell lysis. The resultant microvesicles enlarged and coalesced, forming grossly visible macrovesicles. Mature vesicles progressed to involve all layers of the epithelium but did not involve the underlying dermis. Intradermal inoculation of vesicular exanthema of swine virus type A48 or San Miguel sea lion virus type 2 into otarid (fur) seal pups caused plaque-like lesions around inoculated coronary bands. These swellings regressed without rupture by 96 hours postinoculation. One seal inoculated with San Miguel sea lion virus had a linear lingual erosion at ten days postinoculation. Virus was isolated from this site and from two uninoculated sites, the tonsil and testicle. Contact controls showed no evidence of infection. Virus was isolated in low titers from some sites of inoculation and draining lymph nodes from seals infected with vesicular exanthema of swine virus. Virus was recovered more easily, in higher titers, and from more tissues, from seals infected with San Miguel sea lion virus. Inoculated seals tested after four to ten days seroconverted. Feeding swine seal tissues from the inoculation experiments resulted in seroconversion in swine which were fed tissues from seals infected with vesicular exanthema of swine virus but not in those which were fed tissues from seals infected with San Miguel sea lion virus.

  1. FurIOS: A Web-Based Tool for Identification of Vibrionaceae Species Using the fur Gene

    PubMed Central

    Machado, Henrique; Cardoso, João; Giubergia, Sonia; Rapacki, Kristoffer; Gram, Lone

    2017-01-01

    Gene based methods for identification of species from the Vibrionaceae family have been developed during the last decades to address the limitations of the commonly used 16S rRNA gene phylogeny. Recently, we found that the ferric-uptake regulator gene (fur) can be used as a single identification marker providing species discrimination, consistent with multi-locus sequencing analyses and whole genome phylogenies. To allow for broader and easy use of this marker, we have developed an online prediction service that allows the identification of Vibrionaceae species based on their fur-sequence. The input is a DNA sequence that can be uploaded on the web service; the output is a table containing the strain identifier, e-value, and percentage of identity for each of the matches with rows colored in green for hits with high probability of being the same species. The service is available on the web at: http://www.cbs.dtu.dk/services/furIOS-1.0/. The fur-sequences can be derived either from genome sequences or from PCR-amplification of the genomic region encoding the fur gene. We have used 191 strains identified as Vibrionaceae based on 16S rRNA gene sequence to test the PCR method and the web service on a large dataset. We were able to classify 171 of 191 strains at the species level and 20 strains remained unclassified. Furthermore, the fur phylogenetics and subsequent in silico DNA-DNA hybridization demonstrated that two strains (ATCC 33789 and ZS-139) previously identified as Vibrio splendidus are more closely related to V. tasmaniensis and V. cyclitrophicus, respectively. FurIOS is an easy-to-use online service that allows the identification of bacteria from the Vibrionaceae family at the species level using the fur gene as a single marker. Its simplistic design and straightforward pipeline makes it suitable for any research environment, from academia to industry. PMID:28348552

  2. FurIOS: A Web-Based Tool for Identification of Vibrionaceae Species Using the fur Gene.

    PubMed

    Machado, Henrique; Cardoso, João; Giubergia, Sonia; Rapacki, Kristoffer; Gram, Lone

    2017-01-01

    Gene based methods for identification of species from the Vibrionaceae family have been developed during the last decades to address the limitations of the commonly used 16S rRNA gene phylogeny. Recently, we found that the ferric-uptake regulator gene (fur) can be used as a single identification marker providing species discrimination, consistent with multi-locus sequencing analyses and whole genome phylogenies. To allow for broader and easy use of this marker, we have developed an online prediction service that allows the identification of Vibrionaceae species based on their fur-sequence. The input is a DNA sequence that can be uploaded on the web service; the output is a table containing the strain identifier, e-value, and percentage of identity for each of the matches with rows colored in green for hits with high probability of being the same species. The service is available on the web at: http://www.cbs.dtu.dk/services/furIOS-1.0/. The fur-sequences can be derived either from genome sequences or from PCR-amplification of the genomic region encoding the fur gene. We have used 191 strains identified as Vibrionaceae based on 16S rRNA gene sequence to test the PCR method and the web service on a large dataset. We were able to classify 171 of 191 strains at the species level and 20 strains remained unclassified. Furthermore, the fur phylogenetics and subsequent in silico DNA-DNA hybridization demonstrated that two strains (ATCC 33789 and ZS-139) previously identified as Vibrio splendidus are more closely related to V. tasmaniensis and V. cyclitrophicus, respectively. FurIOS is an easy-to-use online service that allows the identification of bacteria from the Vibrionaceae family at the species level using the fur gene as a single marker. Its simplistic design and straightforward pipeline makes it suitable for any research environment, from academia to industry.

  3. Fuel cell manifold sealing system

    DOEpatents

    Grevstad, Paul E.; Johnson, Carl K.; Mientek, Anthony P.

    1980-01-01

    A manifold-to-stack seal and sealing method for fuel cell stacks. This seal system solves the problem of maintaining a low leak rate manifold seal as the fuel cell stack undergoes compressive creep. The seal system eliminates the problem of the manifold-to-stack seal sliding against the rough stack surface as the stack becomes shorter because of cell creep, which relative motion destroys the seal. The seal system described herein utilizes a polymer seal frame firmly clamped between the manifold and the stack such that the seal frame moves with the stack. Thus, as the stack creeps, the seal frame creeps with it, and there is no sliding at the rough, tough to seal, stack-to-seal frame interface. Here the sliding is on a smooth easy to seal location between the seal frame and the manifold.

  4. SEAL FOR ROTATING SHAFT

    DOEpatents

    Coffman, R.T.

    1957-12-10

    A seal is described for a rotatable shaft that must highly effective when the shaft is not rotating but may be less effective while the shaft is rotating. Weights distributed about a sealing disk secured to the shaft press the sealing disk against a tubular section into which the shiilt extends, and whem the shaft rotates, the centrifugal forces on the weights relieve the pressurc of the sealing disk against the tubular section. This action has the very desirible result of minimizing the wear of the rotating disk due to contact with the tubular section, while affording maximum sealing action when it is needed.

  5. Tamper-indicating seal

    DOEpatents

    Fiarman, S.; Degen, M.F.; Peters, H.F.

    1982-08-13

    There is disclosed a tamper-indicating seal that permits in the field inspection and detection of tampering. Said seal comprises a shrinkable tube having a visible pattern of markings which is shrunk over th item to be sealed, and a second transparent tube, having a second visible marking pattern, which is shrunk over the item and the first tube. The relationship between the first and second set of markings produces a pattern so that the seal may not be removed without detection. The seal is particularly applicable to UF/sub 6/ cylinder valves.

  6. Low Cost, Durable Seal

    SciTech Connect

    Roberts, George; Parsons, Jason; Friedman, Jake

    2010-12-17

    Seal durability is critical to achieving the 2010 DOE operational life goals for both stationary and transportation PEM fuel cell stacks. The seal material must be chemically and mechanically stable in an environment consisting of aggressive operating temperatures, humidified gases, and acidic membranes. The seal must also be producible at low cost. Currentlyused seal materials do not meet all these requirements. This project developed and demonstrated a high consistency hydrocarbon rubber seal material that was able to meet the DOE technical and cost targets. Significant emphasis was placed on characterization of the material and full scale molding demonstrations.

  7. Quantification of the effects of fur, fur color, and velocity on Time-Of-Flight technology in dairy production.

    PubMed

    Salau, Jennifer; Bauer, Ulrike; Haas, Jan H; Thaller, Georg; Harms, Jan; Junge, Wolfgang

    2015-01-01

    With increasing herd sizes, camera based monitoring solutions rise in importance. 3D cameras, for example Time-Of-Flight (TOF) cameras, measure depth information. These additional information (3D data) could be beneficial for monitoring in dairy production. In previous studies regarding TOF technology, only standing cows were recorded to avoid motion artifacts. Therefore, necessary conditions for a TOF camera application in dairy cows are examined in this study. For this purpose, two cow models with plaster and fur surface, respectively, were recorded at four controlled velocities to quantify the effects of movement, fur color, and fur. Comparison criteria concerning image usability, pixel-wise deviation, and precision in coordinate determination were defined. Fur and fur color showed large effects (η (2)=0.235 and η (2)=0.472, respectively), which became even more considerable when the models were moving. The velocity of recorded animals must therefore be controlled when using TOF cameras. As another main result, body parts which lie in the middle of the cow model's back can be determined neglecting the effect of velocity or fur. With this in mind, further studies may obtain sound results using TOF technology in dairy production.

  8. Rotary kiln seal

    SciTech Connect

    Drexler, Robert L.

    1992-01-01

    A rotary seal used to prevent the escape of contaminates from a rotating kiln incinerator. The rotating seal combines a rotating disc plate which is attached to the rotating kiln shell and four sets of non-rotating carbon seal bars housed in a primary and secondary housing and which rub on the sides of the disc. A seal air system is used to create a positive pressure in a chamber between the primary and secondary seals to create a positive air flow into the contaminated gas chamber. The seal air system also employs an air inlet located between the secondary and tertiary seals to further insure that no contaminates pass the seal and enter the external environment and to provide makeup air for the air which flows into the contaminated gas chamber. The pressure exerted by the seal bars on the rotating disc is controlled by means of a preload spring. The seal is capable of operating in a thermally changing environment where the both radial expansion and axial movement of the rotating kiln do not result in the failure of the seal.

  9. COMPRESSION SEAL AND SEALING MATERIAL THEREFOR

    DOEpatents

    Branin, T.G.

    1962-05-29

    This patent relates to compression seal and more particularly to a seaiing material therefor. The sealing surface is a coating consisting of alternate layers of gold and of a non-gold metal having similar plastic flow properties under pressure as gold. The coating is substantially free from oxidation effects when exposed to ambient atmosphere and does not become brittle when worked, as in a valve. (AEC)

  10. Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.

    PubMed

    Baichoo, Noel; Wang, Tao; Ye, Rick; Helmann, John D

    2002-09-01

    The Bacillus subtilis ferric uptake repressor (Fur) protein coordinates a global transcriptional response to iron starvation. We have used DNA microarrays to define the Fur regulon and the iron starvation stimulon. We identify 20 operons (containing 39 genes) that are derepressed both by mutation of fur and by treatment of cells with the iron chelator 2,2'-dipyridyl. These operons are direct targets of Fur regulation as judged by DNase I footprinting. Analyses of lacZ reporter fusions to six Fur-regulated promoter regions reveal that repression is highly selective for iron. In addition to the Fur regulon, iron starvation induces members of the PerR regulon and leads to reduced expression of cytochromes. However, we did not find any evidence for genes that are directly activated by Fur or repressed by Fur under iron-limiting conditions. Although genome searches using the 19 bp Fur box consensus are useful in identifying candidate Fur-regulated genes, some genes associated with Fur boxes are not demonstrably regulated by Fur, whereas other genes are regulated from sites with little apparent similarity to the conventional Fur consensus.

  11. Shaft seal system

    DOEpatents

    Kapich, Davorin D.

    1985-01-01

    A shaft seal system is disclosed for isolating two regions of different fluid mediums through which a rotatable shaft extends. The seal system includes a seal housing through which the shaft extends and which defines an annular land and an annular labyrinth both of which face on the shaft so that each establishes a corresponding fluid sealing annulus. A collection cavity is formed in communication with the annular sealing spaces, and fluids compatible with the fluids in each of the two regions to be isolated are introduced, respectively, into the annular sealing spaces and collected in the collection cavity from which the fluid mixture is removed and passed to a separator which separates the fluids and returns them to their respective annular sealing spaces in a recycling manner. In the illustrated embodiment, the isolated fluid mediums comprise a liquid region and a gas region. Gas is removed from the gas region and passed through a purifier and a gas pump operative to introduce the purified gas through the labyrinth sealing annulus to the collection cavity. After passing to the separator, the separated gas is passed through a dryer from which the dried gas is caused to pass through the labyrinth sealing annulus into the collection cavity independently of the purified gas so as to insure isolation of the gas region in the event of sealing gas pump malfunction.

  12. Contracting/expanding self-sealing cryogenic tube seals

    NASA Technical Reports Server (NTRS)

    Jia, Lin X. (Inventor)

    1997-01-01

    Contracting/expanding self-sealing cryogenic tube seals are disclosed which use the different properties of thermal contraction and expansion of selected dissimilar materials in accord with certain design criteria to yield self-tightening seals via sloped-surface sealing. The seals of the subject invention are reusable, simple to assemble, and adaptable to a wide variety of cryogenic applications.

  13. A functional ferric uptake regulator (Fur) protein in the fish pathogen Piscirickettsia salmonis.

    PubMed

    Almarza, Oscar; Valderrama, Katherine; Ayala, Manuel; Segovia, Cristopher; Santander, Javier

    2016-03-01

    Piscirickettsia salmonis, a Gram-negative fastidious facultative intracellular pathogen, is the causative agent of the salmonid rickettsial septicemia (SRS). The P. salmonis iron acquisition mechanisms and its molecular regulation are unknown. Iron is an essential element for bacterial pathogenesis. Typically, genes that encode for the iron acquisition machinery are regulated by the ferric uptake regulator (Fur) protein. P. salmonis fur sequence database reveals a diversity of fur genes without functional verification. Due to the fastidious nature of this bacterium, we evaluated the functionality of P. salmonis fur in the Salmonella Δfur heterologous system. Although P. salmonis fur gene strongly differed from the common Fur sequences, it restored the regulatory mechanisms of iron acquisition in Salmonella. We concluded that P. salmonis LF-89 has a conserved functional Fur protein, which reinforces the importance of iron during fish infection. [Int Microbiol 2016; 49-55].

  14. Layered seal for turbomachinery

    DOEpatents

    Sarawate, Neelesh Nandkumar; Morgan, Victor John; Weber, David Wayne

    2015-11-20

    The present application provides seal assemblies for reducing leakages between adjacent components of turbomachinery. The seal assemblies may include outer shims, and at least a portion of the outer shims may be substantially impervious. At least one of the outer shims may be configured for sealing engagement with seal slots of the adjacent components. The seal assemblies may also include at least one of an inner shim and a filler layer positioned between the outer shims. The at least one inner shim may be substantially solid and the at least one filler layer may be relatively porous. The seal assemblies may be sufficiently flexible to account for misalignment between the adjacent components, sufficiently stiff to meet assembly requirements, and sufficiently robust to operating meet requirements associated with turbomachinery.

  15. Seals Code Development Workshop

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C. (Compiler); Liang, Anita D. (Compiler)

    1996-01-01

    Seals Workshop of 1995 industrial code (INDSEAL) release include ICYL, GCYLT, IFACE, GFACE, SPIRALG, SPIRALI, DYSEAL, and KTK. The scientific code (SCISEAL) release includes conjugate heat transfer and multidomain with rotordynamic capability. Several seals and bearings codes (e.g., HYDROFLEX, HYDROTRAN, HYDROB3D, FLOWCON1, FLOWCON2) are presented and results compared. Current computational and experimental emphasis includes multiple connected cavity flows with goals of reducing parasitic losses and gas ingestion. Labyrinth seals continue to play a significant role in sealing with face, honeycomb, and new sealing concepts under investigation for advanced engine concepts in view of strict environmental constraints. The clean sheet approach to engine design is advocated with program directions and anticipated percentage SFC reductions cited. Future activities center on engine applications with coupled seal/power/secondary flow streams.

  16. Dynamic sealing principles

    NASA Technical Reports Server (NTRS)

    Zuk, J.

    1976-01-01

    The fundamental principles governing dynamic sealing operation are discussed. Different seals are described in terms of these principles. Despite the large variety of detailed construction, there appear to be some basic principles, or combinations of basic principles, by which all seals function, these are presented and discussed. Theoretical and practical considerations in the application of these principles are discussed. Advantages, disadvantages, limitations, and application examples of various conventional and special seals are presented. Fundamental equations governing liquid and gas flows in thin film seals, which enable leakage calculations to be made, are also presented. Concept of flow functions, application of Reynolds lubrication equation, and nonlubrication equation flow, friction and wear; and seal lubrication regimes are explained.

  17. Mechanically expandable annular seal

    DOEpatents

    Gilmore, R.F.

    1983-07-19

    A mechanically expandable annular reusable seal assembly to form an annular hermetic barrier between two stationary, parallel, and planar containment surfaces is described. A rotatable ring, attached to the first surface, has ring wedges resembling the saw-tooth array of a hole saw. Matching seal wedges are slidably attached to the ring wedges and have their motion restricted to be perpendicular to the second surface. Each seal wedge has a face parallel to the second surface. An annular elastomer seal has a central annular region attached to the seal wedges' parallel faces and has its inner and outer circumferences attached to the first surface. A rotation of the ring extends the elastomer seal's central region perpendicularly towards the second surface to create the fluid tight barrier. A counter rotation removes the barrier. 6 figs.

  18. Mechanically expandable annular seal

    DOEpatents

    Gilmore, Richard F.

    1983-01-01

    A mechanically expandable annular reusable seal assembly to form an annular hermetic barrier between two stationary, parallel, and planar containment surfaces. A rotatable ring, attached to the first surface, has ring wedges resembling the saw-tooth array of a hole saw. Matching seal wedges are slidably attached to the ring wedges and have their motion restricted to be perpendicular to the second surface. Each seal wedge has a face parallel to the second surface. An annular elastomer seal has a central annular region attached to the seal wedges' parallel faces and has its inner and outer circumferences attached to the first surface. A rotation of the ring extends the elastomer seal's central region perpendicularly towards the second surface to create the fluidtight barrier. A counterrotation removes the barrier.

  19. Sodium sulfur battery seal

    DOEpatents

    Mikkor, Mati

    1981-01-01

    This disclosure is directed to an improvement in a sodium sulfur battery construction in which a seal between various battery compartments is made by a structure in which a soft metal seal member is held in a sealing position by holding structure. A pressure applying structure is used to apply pressure on the soft metal seal member when it is being held in sealing relationship to a surface of a container member of the sodium sulfur battery by the holding structure. The improvement comprises including a thin, well-adhered, soft metal layer on the surface of the container member of the sodium sulfur battery to which the soft metal seal member is to be bonded.

  20. Compliant Turbomachine Sealing

    NASA Technical Reports Server (NTRS)

    Hendricks, R. C.; Braun, M. J.; Deng, D.; Hendricks, J. A.

    2011-01-01

    Sealing interface materials and coatings are sacrificial, giving up their integrity for the benefit of the component. Seals that are compliant while still controlling leakage, dynamics, and coolant flows are sought to enhance turbomachine performance. Herein we investigate the leaf-seal configuration. While the leaf seal is classified as contacting, a ready modification using the leaf-housing arrangement in conjunction with an interface film rider (a bore seal, for example) provides for a film-riding noncontact seal. The leaf housing and leaf elements can be made from a variety of materials from plastic to ceramic. Four simplistic models are used to identify the physics essential to controlling leakage. Corroborated by CFD, these results provide design parameters for applications to within reasonable engineering certainty. Some potential improvements are proposed.

  1. Damped flexible seal

    SciTech Connect

    DuBois, Neil J.; Amaral, Antonio M.

    1992-10-27

    A damped flexible seal assembly for a torpedo isolates the tailcone thereof rom vibrational energy present in the drive shaft assembly. A pair of outside flanges, each of which include an inwardly facing groove and an O-ring constrained therein, provide a watertight seal against the outer non-rotating surface of the drive shaft assembly. An inside flange includes an outwardly-facing groove and an O-ring constrained therein, and provides a watertight seal against the inner surface of the tail cone. Two cast-in-place elastomeric seals provide a watertight seal between the flanges and further provide a damping barrier between the outside flanges and the inside flanges for damping vibrational energy present in the drive shaft assembly before the energy can reach the tailcone through the seal assembly.

  2. Inflatable traversing probe seal

    NASA Technical Reports Server (NTRS)

    Trimarchi, Paul A.

    1991-01-01

    An inflatable seal acts as a pressure-tight zipper to provide traversing capability for instrumentation rakes and probes. A specially designed probe segment with a teardrop cross-section in the vicinity of the inflatable seal minimizes leakage at the interface. The probe is able to travel through a lengthwise slot in a pressure vessel or wind tunnel section, while still maintaining pressure integrity. The design uses two commercially available inflatable seals, opposing each other, to cover the probe slot in a wind tunnel wall. Proof-of-concept tests were conducted at vessel pressures up to 30 psig, with seals inflated to 50 psig, showing no measurable leakage along the seal's length or around the probe teardrop cross-section. This seal concept can replace the existing technology of sliding face plate/O-ring systems in applications where lengthwise space is limited.

  3. [Sound reception in marine mammals depending on sound parameters and conduction pathways].

    PubMed

    Babushina, E S

    1999-01-01

    The interaction of complex sounds with the body tissues of Black Sea dolphin (Tursiops truncatus) was studied by the method of instrumental conditioned reflexes with food reinforcement. The thresholds of detecting underwater acoustic signals of different frequencies for dolphin and northern fur seal (Callorhinus ursinus) were measured as a function of pulse duration under conditions of full and partial (head above water) submergence of animals into water. It was found that sound conduction through dolphin tissues was more effective than that in a northern fur seal in a wide frequency range. Presumably, the process of sound propagation in dolphin is accompanied by changes in the amplitude-frequency structure of broad-band sounds. The temporal summation in dolphin hearing was observed at all frequencies under conditions of full and partial submergence, whereas in northern fur seal it was nearly absent at a frequency of 5 kHz under the conditions of head lifting above water.

  4. Liquid Annular Seal Research

    NASA Technical Reports Server (NTRS)

    Palazzolo, Alan B.; Venkataraman, Balaji; Padavala, Sathya S.; Ryan, Steve; Vallely, Pat; Funston, Kerry

    1996-01-01

    This paper highlights the accomplishments on a joint effort between NASA - Marshall Space Flight Center and Texas A and M University to develop accurate seal analysis software for use in rocket turbopump design, design audits and trouble shooting. Results for arbitrary clearance profile, transient simulation, thermal effects solution and flexible seal wall model are presented. A new solution for eccentric seals based on cubic spline interpolation and ordinary differential equation integration is also presented.

  5. Compliant seal development

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C.

    1993-01-01

    The compliant metallic seal combines the noncontact feature of the labyrinth seal, the low leakage of a mechanical seal, and the compliant nature of the brush seal. It consists of several thin metallic elements or leaves mounted within a ring which is press fit into the housing, and in form, sort of resembles a lip seal sections wiping the shaft. A second set of overlapping cover leaves are placed on top of the shaft riding leaves which reduces leakage and provides stiffness. The leaves can be straight or angle cut. The shaft riding fingers are designed with mismatched curvature to provide lift off similar to the Rayleigh lift pads in mechanical seals with leading edge clearances nearly twice those of the trailing edge as as shown by Fleming to be optimal for gas flows in convergent seal passages. Leading edge clearances range from 300 to 500 microinches. Balance pockets beneath the leaves provide fluid film feed to the 'Rayleigh lift' surface and the proper balance ratio (mechanical seal) when combined with the static pressure and film pressure. The leaves flex in the radial direction and accommodate thermomechanical behavior as well as axial motion and angular misalignment. In the static mode, there is a net closing force on the leaves. The seals were tested to 70 psi at speeds to 16,000 rpm or surface speeds to 330 fps and temperatures from ambient to 440 F. A slow cycle through the rig critical at 10,000 rpm induced a radial vibration response of 0.004 to 0.005 inch were accommodated by the seal. Preliminary performance data are encouraging demonstrating hydrodynamic liftoff and noncontacting operation at pressure and speeds typical of gas turbine engines. The leakage performance data are significantly better than commercial labyrinth and brush seals which should be expected as this design incorporates the features of the low leakage face or mechanical seal along with the flexibility of the brush configuration.

  6. Seals Flow Code Development

    NASA Technical Reports Server (NTRS)

    1991-01-01

    In recognition of a deficiency in the current modeling capability for seals, an effort was established by NASA to develop verified computational fluid dynamic concepts, codes, and analyses for seals. The objectives were to develop advanced concepts for the design and analysis of seals, to effectively disseminate the information to potential users by way of annual workshops, and to provide experimental verification for the models and codes under a wide range of operating conditions.

  7. Grayloc seal static tests

    SciTech Connect

    Leisher, W.B.; Biffle, J.H.

    1983-02-01

    A series of evaluation tests was performed on Grayloc seals. Helium service and standard seals, size 292, were used. Measurements were made of axial force and motion, diameter, hoop and axial strain, and helium leak rate. Leak rates were in the 10/sup -6/ atm cc/s range for the helium service seals. Pretest analytical calculations agreed reasonably well with measured makeup forces and deflections.

  8. Quaternary Structure of Fur Proteins, a New Subfamily of Tetrameric Proteins.

    PubMed

    Pérard, Julien; Covès, Jacques; Castellan, Mathieu; Solard, Charles; Savard, Myriam; Miras, Roger; Galop, Sandra; Signor, Luca; Crouzy, Serge; Michaud-Soret, Isabelle; de Rosny, Eve

    2016-03-15

    The ferric uptake regulator (Fur) belongs to the family of the DNA-binding metal-responsive transcriptional regulators. Fur is a global regulator found in all proteobacteria. It controls the transcription of a wide variety of genes involved in iron metabolism but also in oxidative stress or virulence factor synthesis. When bound to ferrous iron, Fur can bind to specific DNA sequences, called Fur boxes. This binding triggers the repression or the activation of gene expression, depending on the regulated genes. As a general view, Fur proteins are considered to be dimeric proteins both in solution and when bound to DNA. In this study, we have purified Fur from four pathogenic strains (Pseudomonas aeruginosa, Francisella tularensis, Yersinia pestis, and Legionella pneumophila) and compared them to Fur from Escherichia coli (EcFur), the best characterized of this family. By using a series of "in solution" techniques, including multiangle laser light scattering and small-angle X-ray scattering, as well as cross-linking experiments, we have shown that the Fur proteins can be classified into two groups, according to their quaternary structure. The group of dimers is represented by EcFur and YpFur and the group of very stable tetramers by PaFur, FtFur, and LpFur. Using PaFur as a case study, we also showed that the dissociation of the tetramers into dimers is necessary for binding of Fur to DNA, and that this dissociation requires the combined effect of metal ion binding and DNA proximity.

  9. Repository seals requirements study

    SciTech Connect

    1997-11-03

    The Yucca Mountain Site Characterization Project, managed by the Civilian Radioactive Waste Management System (CRWMS) Management and Operating Contractor (M and O) is conducting investigations to support the Viability Assessment and the License Application for a high-level nuclear waste repository at Yucca Mountain, Nevada. The sealing subsystem is part of the Yucca Mountain Waste Isolation System. The Yucca Mountain Site Characterization Project is currently evaluating the role of the sealing subsystem (shaft, ramp and exploratory borehole seals) in achieving the overall performance objectives for the Waste Isolation System. This report documents the results of those evaluations. This report presents the results of a repository sealing requirements study. Sealing is defined as the permanent closure of the shafts, ramps, and exploratory boreholes. Sealing includes those components that would reduce potential inflows above the repository, or that would divert flow near the repository horizon to allow vertical infiltration to below the repository. Sealing of such features as emplacement drifts was not done in this study because the current capability to calculate fracture flow into the drifts is not sufficiently mature. The objective of the study is to provide water or air flow performance based requirements for shafts, ramps, and exploratory boreholes located near the repository. Recommendations, as appropriate, are provided for developing plans, seals component testing, and other studies relating to sealing.

  10. Foil Face Seal Testing

    NASA Technical Reports Server (NTRS)

    Munson, John

    2009-01-01

    In the seal literature you can find many attempts by various researchers to adapt film riding seals to the gas turbine engine. None have been successful, potential distortion of the sealing faces is the primary reason. There is a film riding device that does accommodate distortion and is in service in aircraft applications, namely the foil bearing. More specifically a foil thrust bearing. These are not intended to be seals, and they do not accommodate large axial movement between shaft & static structure. By combining the 2 a unique type of face seal has been created. It functions like a normal face seal. The foil thrust bearing replaces the normal primary sealing surface. The compliance of the foil bearing allows the foils to track distortion of the mating seal ring. The foil seal has several perceived advantages over existing hydrodynamic designs, enumerated in the chart. Materials and design methodology needed for this application already exist. Also the load capacity requirements for the foil bearing are low since it only needs to support itself and overcome friction forces at the antirotation keys.

  11. Bidirectional Brush Seals

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C.; Wilson, Jack; Wu, Tom; Flower, Ralph

    1997-01-01

    Presented is a study of the use of a set of I.D./O.D. bidirectional press seals to reduce the leakage losses in a wave rotor. Relative to the baseline configuration, data indicate the use of brush seals enhanced wave rotor efficiency from 36 to 45 percent at low leakages (small rotor endwall gap spacings) and from 15 to 33 percent at high leakages (larger endwall gap spacings). These brush seals are capable of sealing positive or negative pressure drops with respect to the axial direction. Surface tribology for these tests suggested little evidence of grooving although the bristles appeared polished.

  12. Liquid zone seal

    DOEpatents

    Klebanoff, Leonard E.

    2001-01-01

    A seal assembly that provides a means for establishing multiple pressure zones within a system. The seal assembly combines a plate extending from the inner wall of a housing or inner enclosure that intersects with and is immersed in the fluid contained in a well formed in a tray contained within the enclosure. The fluid is a low vapor pressure oil, chemically inert and oxidation resistant. The use of a fluid as the sealing component provides a seal that is self-healing and mechanically robust not subject to normal mechanical wear, breakage, and formation of cracks or pinholes and decouples external mechanical vibrations from internal structural members.

  13. High Pressure Hydraulic Seals

    DTIC Science & Technology

    1988-09-01

    looked XV2690-18D Ring good, front seal XP2714-4 Backups, (ML1841) cracked. American Variseal /Shamban 100,493 110cc leakage on P/N AS-1272 rear seal...BACKUPS SEVERELY SEAL CEC 49S1C-214-DS DETERIORATED. 38. AMERICAN VARISEAL / TESTED THROUGH 100.493 CYCLES. EXCESSIVE LEAKAGE 110 CC SHAMBAN ON REAR SEAL...California 90230 Koppers Company, Inc. Baltimore, Maryland 21203 American Variseal Corp. Broomfield, Colorado 80020 The test actuator parts were serialized

  14. 75 FR 21243 - Marine Mammals; Subsistence Taking of Northern Fur Seals; St. George

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-23

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration RIN 0648-XS60 Marine Mammals; Subsistence Taking of... accessible. Do not submit Confidential Business Information or otherwise sensitive or protected...

  15. Continuous Performance in the Fur Seal: A Bridge to Extended Waking in Humans

    DTIC Science & Technology

    2007-11-29

    Systemic and nasal delivery of orexin -a (hypocretin-1) reduces the effects of sleep deprivation on cognitive performance in nonhuman primates J...continuously active dolphins; Activity and sleep in dolphins (Reply). Nature 441:E11. Siegel JM (2004) Hypocretin ( orexin ): role in normal behavior and

  16. Historical analysis of Newfoundland dog fur colour genetics

    PubMed Central

    Bondeson, J.

    2015-01-01

    This article makes use of digitized historic newspapers to analyze Newfoundland dog fur colour genetics, and fur colour variations over time. The results indicate that contrary to the accepted view, the ‘Solid’ gene was introduced into the British population of Newfoundland dogs in the 1840s. Prior to that time, the dogs were white and black (Landseer) or white and brown, and thus spotted/spotted homozygotes. Due to ‘Solid’ being dominant over ‘spotted’, and selective breeding, today the majority of Newfoundland dogs are solid black. Whereas small white marks on the chest and/or paw appears to be a random event, the historical data supports the existence of an ‘Irish spotted’ fur colour pattern, with white head blaze, breast, paws and tail tip, in spotted/spotted homozygotes. PMID:26623371

  17. Expression of the gonococcal global regulatory protein Fur and genes encompassing the Fur and iron regulon during in vitro and in vivo infection in women.

    PubMed

    Agarwal, Sarika; Sebastian, Shite; Szmigielski, Borys; Rice, Peter A; Genco, Caroline A

    2008-05-01

    The ferric uptake regulatory protein, Fur, functions as a global regulatory protein of gene transcription in the mucosal pathogen Neisseria gonorrhoeae. We have shown previously that several N. gonorrhoeae Fur-repressed genes are expressed in vivo during mucosal gonococcal infection in men, which suggests that this organism infects in an iron-limited environment and that Fur is expressed under these conditions. In this study we have demonstrated expression of the gonococcal fur gene in vitro, in human cervical epithelial cells, and in specimens from female subjects with uncomplicated gonococcal infection. In vitro studies confirmed that the expression of the gonococcal fur gene was repressed during growth under iron-replete growth conditions but that a basal level of the protein was maintained. Using GFP transcriptional fusions constructed from specific Fur binding sequences within the fur promoter/operator region, we determined that this operator region was functional during N. gonorrhoeae infection of cervical epithelial cells. Furthermore, reverse transcription-PCR analysis, as well as microarray analysis, using a custom Neisseria Fur and iron regulon microarray revealed that several Fur- and iron-regulated genes were expressed during N. gonorrhoeae infection of cervical epithelial cells. Microarray analysis of specimens obtained from female subjects with uncomplicated gonococcal infection corroborated our in vitro findings and point toward a key role of gonococcal Fur- and iron-regulated genes in gonococcal disease.

  18. 16 CFR 301.45 - Representations as to construction of fur products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Representations as to construction of fur... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.45 Representations as to construction of fur products. (a) No misleading nor deceptive statements as to...

  19. 19 CFR 11.12a - Labeling of fur products to indicate composition.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Labeling of fur products to indicate composition... SECURITY; DEPARTMENT OF THE TREASURY PACKING AND STAMPING; MARKING Marking § 11.12a Labeling of fur products to indicate composition. (a) Fur products imported into the United States shall have...

  20. 16 CFR 1.63 - Injunctions: Wool, fur, and textile cases.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Injunctions: Wool, fur, and textile cases. 1... PRACTICE GENERAL PROCEDURES Injunctive and Condemnation Proceedings § 1.63 Injunctions: Wool, fur, and textile cases. In those cases arising under the Wool Products Labeling Act of 1939, Fur Products...

  1. 16 CFR 301.45 - Representations as to construction of fur products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Representations as to construction of fur... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.45 Representations as to construction of fur products. (a) No misleading nor deceptive statements as to...

  2. 16 CFR 1.63 - Injunctions: Wool, fur, and textile cases.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Injunctions: Wool, fur, and textile cases. 1... PRACTICE GENERAL PROCEDURES Injunctive and Condemnation Proceedings § 1.63 Injunctions: Wool, fur, and textile cases. In those cases arising under the Wool Products Labeling Act of 1939, Fur Products...

  3. 16 CFR 301.31 - Labeling of fur products consisting of two or more units.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Labeling of fur products consisting of two... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.31 Labeling of fur products consisting of two or more units. (a) The label shall be attached to and...

  4. 16 CFR 301.45 - Representations as to construction of fur products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Representations as to construction of fur... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.45 Representations as to construction of fur products. (a) No misleading nor deceptive statements as to...

  5. 16 CFR 301.31 - Labeling of fur products consisting of two or more units.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Labeling of fur products consisting of two... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.31 Labeling of fur products consisting of two or more units. (a) The label shall be attached to and...

  6. 16 CFR 301.31 - Labeling of fur products consisting of two or more units.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Labeling of fur products consisting of two... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.31 Labeling of fur products consisting of two or more units. (a) The label shall be attached to and...

  7. 16 CFR 301.40 - Item number or mark to be assigned to each fur product.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... fur product. 301.40 Section 301.40 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.40 Item number or mark to be assigned to each fur product. (a) For the purpose of identification, each...

  8. 19 CFR 11.12a - Labeling of fur products to indicate composition.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Labeling of fur products to indicate composition... SECURITY; DEPARTMENT OF THE TREASURY PACKING AND STAMPING; MARKING Marking § 11.12a Labeling of fur products to indicate composition. (a) Fur products imported into the United States shall have...

  9. 16 CFR 1.63 - Injunctions: Wool, fur, and textile cases.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Injunctions: Wool, fur, and textile cases. 1... PRACTICE GENERAL PROCEDURES Injunctive and Condemnation Proceedings § 1.63 Injunctions: Wool, fur, and textile cases. In those cases arising under the Wool Products Labeling Act of 1939, Fur Products...

  10. 19 CFR 11.12a - Labeling of fur products to indicate composition.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Labeling of fur products to indicate composition... SECURITY; DEPARTMENT OF THE TREASURY PACKING AND STAMPING; MARKING Marking § 11.12a Labeling of fur products to indicate composition. (a) Fur products imported into the United States shall have...

  11. 16 CFR 1.63 - Injunctions: Wool, fur, and textile cases.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Injunctions: Wool, fur, and textile cases. 1... PRACTICE GENERAL PROCEDURES Injunctive and Condemnation Proceedings § 1.63 Injunctions: Wool, fur, and textile cases. In those cases arising under the Wool Products Labeling Act of 1939, Fur Products...

  12. 16 CFR 301.40 - Item number or mark to be assigned to each fur product.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... fur product. 301.40 Section 301.40 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.40 Item number or mark to be assigned to each fur product. (a) For the purpose of identification, each...

  13. 16 CFR 301.40 - Item number or mark to be assigned to each fur product.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... fur product. 301.40 Section 301.40 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.40 Item number or mark to be assigned to each fur product. (a) For the purpose of identification, each...

  14. 16 CFR 301.45 - Representations as to construction of fur products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Representations as to construction of fur... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.45 Representations as to construction of fur products. (a) No misleading nor deceptive statements as to...

  15. 16 CFR 301.40 - Item number or mark to be assigned to each fur product.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... fur product. 301.40 Section 301.40 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.40 Item number or mark to be assigned to each fur product. (a) For the purpose of identification, each...

  16. 16 CFR 301.40 - Item number or mark to be assigned to each fur product.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... fur product. 301.40 Section 301.40 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.40 Item number or mark to be assigned to each fur product. (a) For the purpose of identification, each...

  17. 16 CFR 301.45 - Representations as to construction of fur products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Representations as to construction of fur... ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.45 Representations as to construction of fur products. (a) No misleading nor deceptive statements as to...

  18. 19 CFR 11.12a - Labeling of fur products to indicate composition.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Labeling of fur products to indicate composition... SECURITY; DEPARTMENT OF THE TREASURY PACKING AND STAMPING; MARKING Marking § 11.12a Labeling of fur products to indicate composition. (a) Fur products imported into the United States shall have...

  19. 16 CFR 1.63 - Injunctions: Wool, fur, and textile cases.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Injunctions: Wool, fur, and textile cases. 1... PRACTICE GENERAL PROCEDURES Injunctive and Condemnation Proceedings § 1.63 Injunctions: Wool, fur, and textile cases. In those cases arising under the Wool Products Labeling Act of 1939, Fur Products...

  20. 16 CFR 301.31 - Labeling of fur products consisting of two or more units.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Labeling of fur products consisting of two... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.31 Labeling of fur products consisting of two or more units. (a) The label shall be attached to and...

  1. 16 CFR 301.31 - Labeling of fur products consisting of two or more units.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Labeling of fur products consisting of two... SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.31 Labeling of fur products consisting of two or more units. (a) The label shall be attached to and...

  2. 16 CFR 303.9 - Use of fur-bearing animal names and symbols prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Use of fur-bearing animal names and symbols... fur-bearing animal names and symbols prohibited. (a) The advertising or the labeling of a textile... connote or signify a fur-bearing animal, unless such product or the part thereof in connection with...

  3. 16 CFR 303.9 - Use of fur-bearing animal names and symbols prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Use of fur-bearing animal names and symbols... fur-bearing animal names and symbols prohibited. (a) The advertising or the labeling of a textile... connote or signify a fur-bearing animal, unless such product or the part thereof in connection with...

  4. 16 CFR 303.9 - Use of fur-bearing animal names and symbols prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Use of fur-bearing animal names and symbols... fur-bearing animal names and symbols prohibited. (a) The advertising or the labeling of a textile... connote or signify a fur-bearing animal, unless such product or the part thereof in connection with...

  5. 16 CFR 303.9 - Use of fur-bearing animal names and symbols prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Use of fur-bearing animal names and symbols... fur-bearing animal names and symbols prohibited. (a) The advertising or the labeling of a textile... connote or signify a fur-bearing animal, unless such product or the part thereof in connection with...

  6. 16 CFR 303.9 - Use of fur-bearing animal names and symbols prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Use of fur-bearing animal names and symbols... fur-bearing animal names and symbols prohibited. (a) The advertising or the labeling of a textile... connote or signify a fur-bearing animal, unless such product or the part thereof in connection with...

  7. 16 CFR 301.24 - Repairing, restyling and remodeling fur products for consumer.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Repairing, restyling and remodeling fur products for consumer. 301.24 Section 301.24 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS....24 Repairing, restyling and remodeling fur products for consumer. When fur products owned by and...

  8. Regulation of haemolysin (VvhA) production by ferric uptake regulator (Fur) in Vibrio vulnificus: repression of vvhA transcription by Fur and proteolysis of VvhA by Fur-repressive exoproteases.

    PubMed

    Lee, Hyun-Jung; Kim, Jeong-A; Lee, Mi-Ae; Park, Soon-Jung; Lee, Kyu-Ho

    2013-05-01

    VvhA produced by Vibrio vulnificus exhibits cytolytic activity to human cells including erythrocytes. Since haemolysis by VvhA may provide iron for bacterial growth and pathogenicity, we investigated the expression of VvhA to elucidate the regulatory roles of Fur, a major transcription factor controlling iron-homeostasis. Fur repressed the transcription of vvhBA operon via binding to the promoter region. However, haemolysin content and haemolytic activity were lowered in cell-free supernatant of fur mutant. This discrepancy between the levels of vvhA transcript and VvhA protein in fur mutant was caused by exoproteolytic activities of the elastase VvpE and another metalloprotease VvpM, which were also regulated by Fur. vvpE gene expression was repressed by Fur via binding to the Fur-box homologous region. Regulation of VvpM expression by Fur did not occur at the level of vvpM transcription. In vitro proteolysis assays showed that both proteases efficiently degraded VvhA. In addition, the extracellular levels of VvhA were higher in culture supernatants of vvpE or vvpM mutants than in the wild type. Thus this study demonstrates that Fur regulates haemolysin production at the transcription level of the vvhBA operon and at the post-translation level by regulating the expressions of two VvhA-degrading exoproteases, VvpE and VvpM.

  9. Cross-Amplification and Validation of SNPs Conserved over 44 Million Years between Seals and Dogs

    PubMed Central

    Hoffman, Joseph I.; Thorne, Michael A. S.; McEwing, Rob; Forcada, Jaume; Ogden, Rob

    2013-01-01

    High-density SNP arrays developed for humans and their companion species provide a rapid and convenient tool for generating SNP data in closely-related non-model organisms, but have not yet been widely applied to phylogenetically divergent taxa. Consequently, we used the CanineHD BeadChip to genotype 24 Antarctic fur seal (Arctocephalus gazella) individuals. Despite seals and dogs having diverged around 44 million years ago, 33,324 out of 173,662 loci (19.2%) could be genotyped, of which 173 were polymorphic and clearly interpretable. Two SNPs were validated using KASP genotyping assays, with the resulting genotypes being 100% concordant with those obtained from the high-density array. Two loci were also confirmed through in silico visualisation after mapping them to the fur seal transcriptome. Polymorphic SNPs were distributed broadly throughout the dog genome and did not differ significantly in proximity to genes from either monomorphic SNPs or those that failed to cross-amplify in seals. However, the nearest genes to polymorphic SNPs were significantly enriched for functional annotations relating to energy metabolism, suggesting a possible bias towards conserved regions of the genome. PMID:23874599

  10. Resilient Braided Rope Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor); Kren, Lawrence A. (Inventor)

    2000-01-01

    A resilient braided rope seal for use in high temperature applications includes a center core of fibers. a resilient canted spring member supporting the core and at least one layer of braided sheath fibers tightly packed together overlying the spring member. The seal provides both improved load bearing and resiliency. Permanent set and hysteresis are greatly reduced.

  11. Seal ring installation tool

    NASA Technical Reports Server (NTRS)

    Haselmaier, L. Haynes (Inventor)

    2004-01-01

    A seal ring tool that allows an installer to position a primary seal ring between hub ends of pipe flanges that are being assembled together. The tool includes a pivoting handle member and extension arms attached to the pivoting handle member. The ends of the arms have side indentation type longitudinal grooves angled toward one another for holding the primary seal ring in place between the hubs of respective pipes that are to be attached together. The arms of the tool can also have flat sides that can be used to abut against an optional second larger seal that is supported within a groove in one of the hub ends so that the second hub end can then be moved against the other side of the primary seal ring. Once the seal ring is positioned between the pipe hubs, the pipe hubs can be moved about the seal ring due to the flat sides of the arms of the tool. The tool eliminates the chances of damaging and contaminating seal rings being installed within pipe hubs that are being attached to one another.

  12. Pneumatic stowing seals mines

    SciTech Connect

    Brezovec, D.

    1983-11-01

    A pneumatic stowing technique has been used in the US to seal entries to abandoned mines. Limestone mixed with dry cement or bentonite is blown into the opening. Sealing can be accomplished in much less time than with traditional concrete block/clay plug methods.

  13. Seals and Scrolls.

    ERIC Educational Resources Information Center

    Macaulay, Sara Grove

    2000-01-01

    Describes an art unit in which students sculpt a signature seal out of clay and use Chinese brush painting techniques to paint a scroll. Discusses the seal and its historical use in China. Lists materials needed and explains the procedure. (CMK)

  14. Sodium sulfur battery seal

    DOEpatents

    Topouzian, Armenag

    1980-01-01

    This invention is directed to a seal for a sodium sulfur battery in which a flexible diaphragm sealing elements respectively engage opposite sides of a ceramic component of the battery which separates an anode compartment from a cathode compartment of the battery.

  15. The allosteric behavior of Fur mediates oxidative stress signal transduction in Helicobacter pylori.

    PubMed

    Pelliciari, Simone; Vannini, Andrea; Roncarati, Davide; Danielli, Alberto

    2015-01-01

    The microaerophilic gastric pathogen Helicobacter pylori is exposed to oxidative stress originating from the aerobic environment, the oxidative burst of phagocytes and the formation of reactive oxygen species, catalyzed by iron excess. Accordingly, the expression of genes involved in oxidative stress defense have been repeatedly linked to the ferric uptake regulator Fur. Moreover, mutations in the Fur protein affect the resistance to metronidazole, likely due to loss-of-function in the regulation of genes involved in redox control. Although many advances in the molecular understanding of HpFur function were made, little is known about the mechanisms that enable Fur to mediate the responses to oxidative stress. Here we show that iron-inducible, apo-Fur repressed genes, such as pfr and hydA, are induced shortly after oxidative stress, while their oxidative induction is lost in a fur knockout strain. On the contrary, holo-Fur repressed genes, such as frpB1 and fecA1, vary modestly in response to oxidative stress. This indicates that the oxidative stress signal specifically targets apo-Fur repressed genes, rather than impairing indiscriminately the regulatory function of Fur. Footprinting analyses showed that the oxidative signal strongly impairs the binding affinity of Fur toward apo-operators, while the binding toward holo-operators is less affected. Further evidence is presented that a reduced state of Fur is needed to maintain apo-repression, while oxidative conditions shift the preferred binding architecture of Fur toward the holo-operator binding conformation, even in the absence of iron. Together the results demonstrate that the allosteric regulation of Fur enables transduction of oxidative stress signals in H. pylori, supporting the concept that apo-Fur repressed genes can be considered oxidation inducible Fur regulatory targets. These findings may have important implications in the study of H. pylori treatment and resistance to antibiotics.

  16. Repository seals requirement study

    SciTech Connect

    1997-11-03

    The Yucca Mountain Site Characterization Project, managed by the Civilian Radioactive Waste Management System (CRWMS) Management and Operating Contractor (M and O) is conducting investigations to support the Viability Assessment and the License Application for a high-level nuclear waste repository at Yucca Mountain, Nevada. The sealing subsystem is part of the Yucca Mountain Waste Isolation System. The Yucca Mountain Site Characterization Project is currently evaluating the role of the sealing subsystem (shaft, ramp and exploratory borehole seals) in achieving the overall performance objectives for the Waste Isolation System. This report documents the results of those evaluations. The objective of the study is to provide water or air flow performance based requirements for shafts, ramps, and exploratory boreholes located near the repository. Recommendations, as appropriate, are provided for developing plans, seals component testing, and other studies relating to sealing.

  17. Sealing in Turbomachinery

    NASA Technical Reports Server (NTRS)

    Chupp, Raymond E.; Hendricks, Robert C.; Lattime, Scott B.; Steinetz, Bruce M.

    2006-01-01

    Clearance control is of paramount importance to turbomachinery designers and is required to meet today's aggressive power output, efficiency, and operational life goals. Excessive clearances lead to losses in cycle efficiency, flow instabilities, and hot gas ingestion into disk cavities. Insufficient clearances limit coolant flows and cause interface rubbing, overheating downstream components and damaging interfaces, thus limiting component life. Designers have put renewed attention on clearance control, as it is often the most cost effective method to enhance system performance. Advanced concepts and proper material selection continue to play important roles in maintaining interface clearances to enable the system to meet design goals. This work presents an overview of turbomachinery sealing to control clearances. Areas covered include: characteristics of gas and steam turbine sealing applications and environments, benefits of sealing, types of standard static and dynamics seals, advanced seal designs, as well as life and limitations issues.

  18. Rotary shaft seal

    DOEpatents

    Langebrake, C.O.

    1984-01-01

    The invention is a novel rotary shaft seal assembly which provides positive-contact sealing when the shaft is not rotated and which operates with its sealing surfaces separated by a film of compressed ambient gas whose width is independent of the speed of shaft rotation. In a preferred embodiment, the assembly includes a disc affixed to the shaft for rotation therewith. Axially movable, non-rotatable plates respectively supported by sealing bellows are positioned on either side of the disc to be in sealing engagement therewith. Each plate carries piezoelectric transucer elements which are electrically energized at startup to produce films of compressed ambient gas between the confronting surfaces of the plates and the disc. Following shutdown of the shaft, the transducer elements are de-energized. A control circuit responds to incipient rubbing between the plate and either disc by altering the electrical input to the transducer elements to eliminate rubbing.

  19. Rotary shaft seal

    DOEpatents

    Langebrake, Clair O.

    1984-01-01

    The invention is a novel rotary shaft seal assembly which provides positive-contact sealing when the shaft is not rotated and which operates with its sealing surfaces separated by a film of compressed ambient gas whose width is independent of the speed of shaft rotation. In a preferred embodiment, the assembly includes a disc affixed to the shaft for rotation therewith. Axially movable, non-rotatable plates respectively supported by sealing bellows are positioned on either side of the disc to be in sealing engagement therewith. Each plate carries piezoelectric transducer elements which are electrically energized at startup to produce films of compressed ambient gas between the confronting surfaces of the plates and the disc. Following shutdown of the shaft, the transducer elements are de-energized. A control circuit responds to incipient rubbing between the plate and either disc by altering the electrical input to the transducer elements to eliminate rubbing.

  20. Transmission seal development

    NASA Technical Reports Server (NTRS)

    Brien, M.

    1977-01-01

    An experimental evaluation was performed on a high-speed (72.9 m/s, 14,349 ft/min) transmission seal of the synergistic type. During testing of the seal, oil leakage occurred at positive bearing cavity pressures. Modifications were made in an attempt to eliminate the leakage but none were completely successful. Leakage appears to be the result of questionable positioning of the sealing elements resulting in inadequate shaft contact by the oil side sealing element. This condition may be related to the nonsymmetrical shape of the elastomeric retainer and to dimensional changes caused by swelling of the elastomeric retainer from exposure to the sealed fluid. Indications of a speed dependent leakage characteristic were also observed.

  1. SSME interstage seal research

    NASA Technical Reports Server (NTRS)

    Childs, D. W.

    1984-01-01

    Test results comprising direct and transverse force coefficients and leakage coefficients are reported for six seal configurations. All seals tested use the same smooth rotor and have the same constant minimum clearance. The following stator configurations were tested: (1) Smooth, (2) knurled pattern, (3) axially-grooved pattern with end seals, (4) diamond-grid roughened, (5) diamond-grid roughened with end seals, and (6) round-hole pattern. Comparison of the seals shows the Knurled-pattern stator to be the stiffest and the round-hole pattern stator to yield the largest net damping and the least leakage. The theory of reference is shown to substantially underestimate the stiffness and effective-added-mass coefficients, but do a reasonable job in predicting the net-damping-force coefficient.

  2. HSCT Anticipated Seal Needs Turbomachinery Seals Combustor Seals

    NASA Technical Reports Server (NTRS)

    Henry, John

    2006-01-01

    The High Speed Civil Transport (HSCT) engine concept is a large mixed flow turbofan similar in construction to current military fighter engines. The mission, however, is quite different. The engine will operate for long periods of time at very high Mach numbers and high altitudes. The engine is required to have very low emissions and noise levels to be acceptable in commercial service. Current thrust levels are in the 55000 lb range. At the current supercruise speed requirement of Mach 2.4, the engine inlet temperature will be at least 380 F. This is the lowest cycle temperature expected anywhere in the propulsion system.Seals will be exposed to operate at this temperature and higher for thousands of hours without failure. Durability, cost, and weight will all be very important in determining the type of seals selected for a successful HSCT engine.

  3. 8. VIEW OF STUDDING, EXTERIOR WALL FURRING AND LINTELS FOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. VIEW OF STUDDING, EXTERIOR WALL FURRING AND LINTELS FOR NEW WINDOW OPENINGS. NORTH WING, FIRST FLOOR DURING REHABILITATION OF HOSPITAL BUILDING, 1938. PLEASE CREDIT: BUREAU OF YARDS AND DOCKS, NATIONAL ARCHIVES - U. S. Naval Asylum, Laning Hall, Gray's Ferry Avenue, Philadelphia, Philadelphia County, PA

  4. 50 CFR 31.17 - Disposal of furs and pelts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Disposal of furs and pelts. 31.17 Section 31.17 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) THE NATIONAL WILDLIFE REFUGE SYSTEM WILDLIFE SPECIES MANAGEMENT Terms and Conditions of...

  5. Discovery of Fur binding site clusters in Escherichia coli by information theory models

    PubMed Central

    Chen, Zehua; Lewis, Karen A.; Shultzaberger, Ryan K.; Lyakhov, Ilya G.; Zheng, Ming; Doan, Bernard; Storz, Gisela; Schneider, Thomas D.

    2007-01-01

    Fur is a DNA binding protein that represses bacterial iron uptake systems. Eleven footprinted Escherichia coli Fur binding sites were used to create an initial information theory model of Fur binding, which was then refined by adding 13 experimentally confirmed sites. When the refined model was scanned across all available footprinted sequences, sequence walkers, which are visual depictions of predicted binding sites, frequently appeared in clusters that fit the footprints (∼83% coverage). This indicated that the model can accurately predict Fur binding. Within the clusters, individual walkers were separated from their neighbors by exactly 3 or 6 bases, consistent with models in which Fur dimers bind on different faces of the DNA helix. When the E. coli genome was scanned, we found 363 unique clusters, which includes all known Fur-repressed genes that are involved in iron metabolism. In contrast, only a few of the known Fur-activated genes have predicted Fur binding sites at their promoters. These observations suggest that Fur is either a direct repressor or an indirect activator. The Pseudomonas aeruginosa and Bacillus subtilis Fur models are highly similar to the E. coli Fur model, suggesting that the Fur–DNA recognition mechanism may be conserved for even distantly related bacteria. PMID:17921503

  6. Analysis of a ferric uptake regulator (Fur) knockout mutant in Aeromonas salmonicida subsp. salmonicida.

    PubMed

    Ebanks, Roger O; Goguen, Michel; Knickle, Leah; Dacanay, Andrew; Leslie, Andrew; Ross, Neil W; Pinto, Devanand M

    2013-03-23

    Aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis; a serious infectious disease in aquaculture raised salmonids. Iron acquisition has been shown to be critical for the survival of pathogenic bacteria during the course of infection. Previous work has demonstrated that A. salmonicida expresses iron-repressible IROMP proteins, suggesting the presence of iron acquisition systems that are under the control of a ferric uptake regulator (Fur). In this study, the A. salmonicida fur has been sequenced and a fur deletion strain generated. The A. salmonicida fur gene has an open reading frame of 428 bp, coding for a protein of 143 amino acids, and with high homology to previously described Fur proteins. The Fur protein product had a 94% sequence identity and 96% sequence similarity to the Aeromonas hydrophila Fur protein product. Transcription of the A. salmonicida fur gene was not regulated by the iron status of the bacterium and is not autoregulated, as in Escherichia coli. Proteomic analysis of the A. salmonicida fur mutant, fails to repress iron-regulated outer membrane proteins in the presence of iron. The A. salmonicida fur::KO mutant shows significantly reduced pathogenicity compared to the wild-type parental strain. In addition, the A. salmonicida fur mutant provides an important tool for further investigation of the iron acquisition mechanisms utilized by A. salmonicida.

  7. Identification of the fur-binding site in regulatory region of the vulnibactin-receptor gene in Vibrio vulnificus.

    PubMed

    Lee, Hyun-Jung; Lee, Kyu-Ho

    2012-01-01

    The Vibrio vulnificus vuuA gene, of which expression is repressed by a complex of iron and ferric uptake regulator (Fur), was characterized to localize the Fur-binding site in its upstream regulatory region. In silico analysis suggested the presence of two possible Fur-binding sites; one is a classical Fur-box and the other is a previously reported distinct Fur-binding site. Site-directed mutagenesis and DNase I protection assays revealed the binding site for the iron-Fur complex, which includes an extended inverted repeat containing a homologous sequence to the classical Fur-box.

  8. Seals Flow Code Development 1993

    NASA Technical Reports Server (NTRS)

    Liang, Anita D. (Compiler); Hendricks, Robert C. (Compiler)

    1994-01-01

    Seals Workshop of 1993 code releases include SPIRALI for spiral grooved cylindrical and face seal configurations; IFACE for face seals with pockets, steps, tapers, turbulence, and cavitation; GFACE for gas face seals with 'lift pad' configurations; and SCISEAL, a CFD code for research and design of seals of cylindrical configuration. GUI (graphical user interface) and code usage was discussed with hands on usage of the codes, discussions, comparisons, and industry feedback. Other highlights for the Seals Workshop-93 include environmental and customer driven seal requirements; 'what's coming'; and brush seal developments including flow visualization, numerical analysis, bench testing, T-700 engine testing, tribological pairing and ceramic configurations, and cryogenic and hot gas facility brush seal results. Also discussed are seals for hypersonic engines and dynamic results for spiral groove and smooth annular seals.

  9. Reactor cavity seal ring

    SciTech Connect

    Hankinson, M.F.

    1986-04-22

    A hydrostatic seal is described for sealing an annular gap between two flat substantially horizontal coplanar surfaces comprising, in combination: a generally flat annular plate of a width sufficient to span a gap between two surfaces: compressible annular sealing means disposed on the bottom surface of the flat annular plate for sealingly engaging the two flat surfaces in response to a downward force exerted on the plate; and fastening means, distributed along the center line of the plate, for releasably fastening the plate in a position to span the gap to be sealed and exert a downward force on the plate, each fastening means including a pair of elongated members of a size to fit into the gap to be sealed, means for mounting the members on the bottom surface of the plate so that at least a portion of each member is radially moveable in a direction toward a respective one of the vertical side surfaces defining the gap to be sealed to engage same and so that the plate is moveable relative to the members in a downward direction in response to hydrostatic pressure applied to the upper surface of the plate when the members are engaging the vertical side surfaces of an annular gap, and an actuating means, mounted on the plate for movement therewith in response to hydrostatic pressure, for radially moving the members, the actuating means extending through a bore in the plate to the upper surface of the plate.

  10. Origins of specificity and cross-talk in metal ion sensing by Bacillus subtilis Fur.

    PubMed

    Ma, Zhen; Faulkner, Melinda J; Helmann, John D

    2012-12-01

    Fur (ferric uptake regulator) is the master regulator of iron homeostasis in many bacteria, but how it responds specifically to Fe(II) in vivo is not clear. Biochemical analyses of Bacillus subtilis Fur (BsFur) reveal that in addition to Fe(II), both Zn(II) and Mn(II) allosterically activate BsFur-DNA binding. Dimeric BsFur co-purifies with site 1 structural Zn(II) (Fur(2) Zn(2) ) and can bind four additional Zn(II) or Mn(II) ions per dimer. Metal ion binding at previously described site 3 occurs with highest affinity, but the Fur(2) Zn(2) :Me(2) form has only a modest increase in DNA binding affinity (approximately sevenfold). Metallation of site 2 (Fur(2) Zn(2) :Me(4) ) leads to a ~ 150-fold further enhancement in DNA binding affinity. Fe(II) binding studies indicate that BsFur buffers the intracellular Fe(II) concentration at ~ 1 μM. Both Mn(II) and Zn(II) are normally buffered at levels insufficient for metallation of BsFur site 2, thereby accounting for the lack of cross-talk observed in vivo. However, in a perR mutant, where the BsFur concentration is elevated, BsFur may now use Mn(II) as a co-repressor and inappropriately repress iron uptake. Since PerR repression of fur is enhanced by Mn(II), and antagonized by Fe(II), PerR may co-regulate Fe(II) homeostasis by modulating BsFur levels in response to the Mn(II)/Fe(II) ratio.

  11. Sealing device for providing a seal in a turbomachine

    DOEpatents

    Lacy, Benjamin Paul; Kottilingam, Srikanth Chandrudu; Porter, Christopher Donald; Schick, David Edward; Weber, David Wayne

    2016-08-16

    Sealing device for providing seals between adjacent components, and turbomachines utilizing such sealing devices, are provided. A sealing device includes a seal plate insertable between the adjacent components, the seal plate comprising a first face and an opposing second face. The sealing device further includes a plurality of pins extending from one of the first face or the second face, the plurality of pins configured to space the one of the first face or the second face from contact surfaces of the adjacent components.

  12. Multilayer compressive seal for sealing in high temperature devices

    DOEpatents

    Chou, Yeong-Shyung; Stevenson, Jeffry W.

    2007-08-21

    A mica based compressive seal has been developed exhibiting superior thermal cycle stability when compared to other compressive seals known in the art. The seal is composed of compliant glass or metal interlayers and a sealing (gasket) member layer composed of mica that is infiltrated with a glass forming material, which effectively reduces leaks within the seal. The compressive seal shows approximately a 100-fold reduction in leak rates compared with previously developed hybrid seals after from 10 to about 40 thermal cycles under a compressive stress of from 50 psi to 100 psi at temperatures in the range from 600.degree. C. to about 850.degree. C.

  13. Bellow seal and anchor

    DOEpatents

    Mansure, Arthur J.

    2001-01-01

    An annular seal is made of a collapsible bellows. The bellows can function as an anchor or a seal and is easily set into position using relative component movement. The bellows folds can be slanted and their outer sealing edges can have different profiles to meet expected conditions. The bellows is expanded for insertion to reduce its outer dimension and sets by compaction as a result of relative movement. The bellows can be straight or tapered and is settable with a minimal axial force.

  14. Gland With Cantilever Seal

    NASA Technical Reports Server (NTRS)

    Melton, Patrick B.

    1989-01-01

    Single-piece gland forms tight seal on probe or tube containing liquid or gas at high pressure. Gland and probe align as assembled by simple torquing procedure. Disconnected easily and reused at same site. Made from any of wide variety of materials so compatible with application. Cantilever ring at top of gland bites into wall of tube or probe, sealing it. Wall of tube or probe must be thick enough to accommodate deformation without rupturing. Maximum deformation designed in coordination with seating and deformation of boss or conical seal.

  15. SEALING SIMULATED LEAKS

    SciTech Connect

    Michael A. Romano

    2004-09-01

    This report details the testing equipment, procedures and results performed under Task 7.2 Sealing Simulated Leaks. In terms of our ability to seal leaks identified in the technical topical report, Analysis of Current Field Data, we were 100% successful. In regards to maintaining seal integrity after pigging operations we achieved varying degrees of success. Internal Corrosion defects proved to be the most resistant to the effects of pigging while External Corrosion proved to be the least resistant. Overall, with limitations, pressure activated sealant technology would be a viable option under the right circumstances.

  16. Fur Trade Era Interpretation. The Voyageur Sash and Garter and a Fur Trade Emblem: A Curiosity Ripe for Interpretation.

    ERIC Educational Resources Information Center

    Davis, Wendy; Henderson, Bob

    1998-01-01

    Provides outdoor educators with background information for historical interpretation activities during Canadian canoe trips. Discusses the history, evolution, and uses of brightly colored sashes and garters worn by voyageurs during the fur trade era, and speculates on the origins of the Lauburu canoe emblem, used by both the Mic Mac peoples and…

  17. Fur Production as a Specialized Activity in a World System: Indians in the North American Fur Trade.

    ERIC Educational Resources Information Center

    Kardulias, P. Nick

    1990-01-01

    Examines the fur trade as critical fulcrum in Indian-White contact, focusing on craft specialization as the Native response to the international market system. Describes effects on Indian dependence on European trade goods, Native territorial boundaries and population distribution, social and family structures, and animal populations. Contains 73…

  18. Engine sealing and lubrication systems

    NASA Technical Reports Server (NTRS)

    Zuk, J.

    1975-01-01

    Engine sealing programs are discussed which are directed toward the two major classes of engine seals: engine shaft seals and primary gas path seals. In addition, some concepts and results from fundamental lubrication research, as it pertains to the lubrication of bearings, are presented.

  19. Photomicrographic images of some features of Uncinaria spp (Nematoda: Ancylostomatidae) from otariid pinnipeds.

    PubMed

    Lyons, E T; DeLong, R L

    2005-03-01

    Photomicrographs of several morphologic features of hookworms (Uncinaria spp) from northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups are presented. The main purpose is to show and describe some physical characteristics of hookworms from the two hosts; it is not to decide from these attributes whether the Uncinaria spp are the same species. The number of species of Uncinaria in pinnipeds is uncertain and specimens need to be examined from the various infected seals and sea lions before the taxonomy of these parasites can be clarified. Information in the present paper should aid in this determination.

  20. Tamper indicating seal

    DOEpatents

    Romero, Juan A [Albuquerque, NM; Walker, Charles A [Albuquerque, NM; Blair, Dianna S [Albuquerque, NM; Bodmer, Connie C [Albuquerque, NM

    2012-05-29

    Seals have a flexible wire that can be looped through a hasp-like device. The seals include a body having a recess, a plug insertable into the recess and a snap ring for fastening the plug to the body. The plug and/or body can have access holes for inserting the wire into the recess. "Teeth" on the outer diameter and through-holes through the thickness of the snap ring allow for passing the ends of the flexible wire from the recess through the snap ring. The ends of the wire can be folded back over the snap ring and into engagement with the teeth. Assembly of the seal causes the ends of the wire to be securely fastened between the teeth of the snap ring and the sidewall of the recess. Seals can include a plug and/or body made of a frangible material such as glass, ceramic, glass-ceramic or brittle polymer.

  1. Gas turbine sealing apparatus

    SciTech Connect

    Marra, John Joseph; Wessell, Brian J.; Liang, George

    2013-03-05

    A sealing apparatus in a gas turbine. The sealing apparatus includes a seal housing apparatus coupled to a disc/rotor assembly so as to be rotatable therewith during operation of the gas turbine. The seal housing apparatus comprises a base member, a first leg portion, a second leg portion, and spanning structure. The base member extends generally axially between forward and aft rows of rotatable blades and is positioned adjacent to a row of stationary vanes. The first leg portion extends radially inwardly from the base member and is coupled to the disc/rotor assembly. The second leg portion is axially spaced from the first leg portion, extends radially inwardly from the base member, and is coupled to the disc/rotor assembly. The spanning structure extends between and is rigidly coupled to each of the base member, the first leg portion, and the second leg portion.

  2. Flexible cloth seal assembly

    DOEpatents

    Bagepalli, B.S.; Taura, J.C.; Aksit, M.F.; Demiroglu, M.; Predmore, D.R.

    1999-06-29

    A seal assembly is described having a flexible cloth seal which includes a shim assemblage surrounded by a cloth assemblage. A first tubular end portion, such as a gas turbine combustor, includes a longitudinal axis and has smooth and spaced-apart first and second surface portions defining a notch there between which is wider at its top than at its bottom and which extends outward from the axis. The second surface portion is outside curved, and a first edge of the cloth seal is positioned in the bottom of the notch. A second tubular end portion, such as a first stage nozzle, is located near, spaced apart from, and coaxially aligned with, the first tubular end portion. The second tubular end portion has a smooth third surface portion which surrounds at least a portion of the first tubular end portion and which is contacted by the cloth seal. 7 figs.

  3. Rotatable seal assembly

    DOEpatents

    Logan, Clinton M.; Garibaldi, Jack L.

    1982-01-01

    An assembly is provided for rotatably supporting a rotor on a stator so that vacuum chambers in the rotor and stator remain in communication while the chambers are sealed from ambient air, which enables the use of a ball bearing or the like to support most of the weight of the rotor. The apparatus includes a seal device mounted on the rotor to rotate therewith, but shiftable in position on the rotor while being sealed to the rotor as by an O-ring. The seal device has a flat face that is biased towards a flat face on the stator, and pressurized air is pumped between the faces to prevent contact between them while spacing them a small distance apart to avoid the inflow of large amounts of air between the faces and into the vacuum chambers.

  4. Flexible cloth seal assembly

    DOEpatents

    Bagepalli, Bharat Sampathkumar; Taura, Joseph Charles; Aksit, Mahmut Faruk; Demiroglu, Mehmet; Predmore, Daniel Ross

    1999-01-01

    A seal assembly having a flexible cloth seal which includes a shim assemblage surrounded by a cloth assemblage. A first tubular end portion, such as a gas turbine combustor, includes a longitudinal axis and has smooth and spaced-apart first and second surface portions defining a notch therebetween which is wider at its top than at its bottom and which extends outward from the axis. The second surface portion is outside curved, and a first edge of the cloth seal is positioned in the bottom of the notch. A second tubular end portion, such as a first stage nozzle, is located near, spaced apart from, and coaxially aligned with, the first tubular end portion. The second tubular end portion has a smooth third surface portion which surrounds at least a portion of the first tubular end portion and which is contacted by the cloth seal.

  5. Turbine seal assembly

    SciTech Connect

    Little, David A.

    2013-04-16

    A seal assembly that limits gas leakage from a hot gas path to one or more disc cavities in a turbine engine. The seal assembly includes a seal apparatus that limits gas leakage from the hot gas path to a respective one of the disc cavities. The seal apparatus comprises a plurality of blade members rotatable with a blade structure. The blade members are associated with the blade structure and extend toward adjacent stationary components. Each blade member includes a leading edge and a trailing edge, the leading edge of each blade member being located circumferentially in front of the blade member's corresponding trailing edge in a direction of rotation of the turbine rotor. The blade members are arranged such that a space having a component in a circumferential direction is defined between adjacent circumferentially spaced blade members.

  6. Ingestion resistant seal assembly

    SciTech Connect

    Little, David A

    2011-12-13

    A seal assembly limits gas leakage from a hot gas path to one or more disc cavities in a gas turbine engine. The seal assembly includes a seal apparatus associated with a blade structure including a row of airfoils. The seal apparatus includes an annular inner shroud associated with adjacent stationary components, a wing member, and a first wing flange. The wing member extends axially from the blade structure toward the annular inner shroud. The first wing flange extends radially outwardly from the wing member toward the annular inner shroud. A plurality of regions including one or more recirculation zones are defined between the blade structure and the annular inner shroud that recirculate working gas therein back toward the hot gas path.

  7. Conduit sealing system

    SciTech Connect

    Weinberg, R.A.

    1984-02-28

    The invention relates to an annular seal system designed for high pressure applications in subterranean wells. The annular seal system comprises a vertical stack of subassemblies. Each subassembly incorporates an annular sealing element formed from an elastomeric material, such as a perfluoroelastomer, which is provided with a truncated pear-shaped cross-sectional configuration having reversely curved axial side surfaces. The sealing element is abutted on each axial side by a uniform thickness annular bearing element formed from a thermoplastic such as a polyphenylene sulfide resin having good bearing properties. Each of the thermoplastic bearing elements is in turn abutted by an annular metallic restraining element having correspondingly shaped reversely curved axial side surfaces and defining an inverted truncated pear-shaped cross-sectional configuration.

  8. Current developments in brush seals

    NASA Technical Reports Server (NTRS)

    Loewenthal, Bob

    1994-01-01

    The objective of the brush seal development program is to develop a comprehensive design methodology for brush seals using application requirements from engine manufacturers and experimental characterization of seal design and tribological pairs. The goals are to substantially lower leakage compared to labyrinth seals, seal life consistent with man-rated mission requirements, to investigate single and multiple staged brush seals, temperature up to 1200 F and surface speed up to 900 fps, and pressure drop across the seal of 50 psid. Test results are presented in viewgraph format.

  9. Ultra high vacuum seal arrangement

    DOEpatents

    Flaherty, Robert

    1981-01-01

    Arrangement for demountably sealing two concentric metallic tubes in an ultra high vacuum system which facilitates remote actuation. A tubular seal includes integral spaced lips which circumferentially engage the metallic tubes. The lips plastically deform the metallic tubes by mechanical forces resulting from a martensite to austenite transformation of the tubular seal upon application of a predetermined temperature. The sealing force is released upon application of another temperature which causes a transformation from the stronger austenite to the weaker martensite. Use of a dual acting sealing ring and driving ring circumferentially contacting the sealing ring is particularly applicable to sealing larger diameter concentric metallic members.

  10. Seal system with integral detector

    DOEpatents

    Fiarman, S.

    1982-08-12

    A seal system is disclosed for materials where security is of the essence, such as nuclear materials. The seal is tamper-indicating, indicates changes in environmental conditions that evidence attempts to bypass the seal, is unique and cost effective. The seal system is comprised of a seal where an optical signal is transmitted through a loop, with a detector to read said signal, and one or more additional detectors designed to detect environmental changes, these detectors being operatively associated with the seal so that detection of a break in the optical signal or detection of environmental changes will cause an observable change in the seal.

  11. Self-acting shaft seals

    NASA Technical Reports Server (NTRS)

    Ludwig, L. P.

    1978-01-01

    Self-acting seals are described in detail. The mathematical models for obtaining a seal force balance and the equilibrium operating film thickness are outlined. Particular attention is given to primary ring response (seal vibration) to rotating seat face runout. This response analysis reveals three different vibration models with secondary seal friction being an important parameter. Leakage flow inlet pressure drop and affects of axisymmetric sealing face deformations are discussed. Experimental data on self-acting face seals operating under simulated gas turbine conditions are given. Also a spiral groove seal design operated to 244 m/sec (800 ft/sec) is described.

  12. Core disruptive accident margin seal

    DOEpatents

    Golden, Martin P.

    1979-01-01

    Apparatus for sealing the annulus defined within a substantially cylindrical rotatable riser assembly and plug combination of a nuclear reactor closure head. The apparatus comprises an inflatable sealing mechanism disposed in one portion of the riser assembly near the annulus such that upon inflation the sealing mechanism is radially actuated against the other portion of the riser assembly thereby sealing the annulus. The apparatus further comprises a connecting mechanism which places one end of the sealing mechanism in fluid communication with the reactor cover gas so that overpressurization of the reactor cover gas will increase the radial actuation of the sealing mechanism thus enhancing sealing of the annulus.

  13. Radial pressure flange seal

    DOEpatents

    Batzer, Thomas H.; Call, Wayne R.

    1989-01-01

    This invention provides an all metal seal for vacuum or pressure vessels or systems. This invention does not use gaskets. The invention uses a flange which fits into a matching groove. Fluid pressure is applied in a chamber in the flange causing at least one of the flange walls to radially press against a side of the groove creating the seal between the flange wall and the groove side.

  14. Radial pressure flange seal

    DOEpatents

    Batzer, T.H.; Call, W.R.

    1989-01-24

    This invention provides an all metal seal for vacuum or pressure vessels or systems. This invention does not use gaskets. The invention uses a flange which fits into a matching groove. Fluid pressure is applied in a chamber in the flange causing at least one of the flange walls to radially press against a side of the groove creating the seal between the flange wall and the groove side. 5 figs.

  15. Titanium hermetic seals

    DOEpatents

    Brow, Richard K.; Watkins, Randall D.

    1995-01-01

    Titanium is prenitrided by being heated in a nitrogen environment under conditions which give rise to the formation of a titanium-nitride surface layer on the titanium. Titanium thus prenitrided may be used in electrical components which are hermetically sealed using silicate glasses and standard glass sealing techniques. According to the method of the invention, alkali volatilization and formation of deleterious interfacial silicide are inhibited.

  16. Titanium hermetic seals

    DOEpatents

    Brow, Richard K.; Watkins, Randall D.

    1995-07-04

    Titanium is prenitrided by being heated in a nitrogen environment under conditions which give rise to the formation of a titanium-nitride surface layer on the titanium. Titanium thus prenitrided may be used in electrical components which are hermetically sealed using silicate glasses and standard glass sealing techniques. According to the method of the invention, alkali volatilization and formation of deleterious interfacial silicide are inhibited.

  17. Applauding the performing seal

    SciTech Connect

    Not Available

    1992-03-01

    Whether the culprit is a pump, valve, compressor, or flanged pipe connection, the end result is the same: process-fluid leakage damages the environment and is costly to operators, in terms of lost product, lost production due to downtime, and the replacement of damaged process equipment. Hard-to-handle fluids, such as abrasive slurries of coal and flyash, can damage the seals that contain them, allowing potentially harmful fluids to escape. Even more insidious is the often-invisible escape of volatile organic compounds (VOCs) from equipment that handles liquid petroleum, petroleum by-products and chemicals. This paper reports that seal manufacturers are using their ingenuity to minimize the escape of liquid and gaseous emissions. One approach uses the power of magnetic attraction to create a seal along a rotating pump shaft by positioning magnetic fluids there. Ferrofluidics Corp. starts by creating a magnetic fluid - essentially a colloidal suspension of ultrafine magnetic salts dispersed in a carrier fluid - which is then used as a liquid O-ring seal in conjunction with a mechanical seal. The seal apparatus consists of a doughnut-shaped sandwich (centered around the pump shaft), with a permanent magnet as the middle layer, and a washer of magnetically permeable material on either side.

  18. Nuclear instrumentation cable end seal

    DOEpatents

    Cannon, Collins P.; Brown, Donald P.

    1979-01-01

    An improved coaxial end seal for hermetically sealed nuclear instrumentation cable exhibiting an improved breakdown pulse noise characteristic under high voltage, high temperature conditions. A tubular insulator body has metallized interior and exterior surface portions which are braze sealed to a center conductor and an outer conductive sheath. The end surface of the insulator body which is directed toward the coaxial cable to which it is sealed has a recessed surface portion within which the braze seal material terminates.

  19. Identification of three novel antisense RNAs in the fur locus from unicellular cyanobacteria.

    PubMed

    Sevilla, Emma; Martín-Luna, Beatriz; González, Andrés; Gonzalo-Asensio, Jesús A; Peleato, María Luisa; Fillat, María F

    2011-12-01

    The interplay between Fur (ferric uptake regulator) proteins and small, non-coding RNAs has been described as a key regulatory loop in several bacteria. In the filamentous cyanobacterium Anabaena sp. PCC 7120, a large dicistronic transcript encoding the putative membrane protein Alr1690 and an α-furA RNA is involved in the modulation of the global regulator FurA. In this work we report the existence of three novel antisense RNAs in cyanobacteria and show that a cis α-furA RNA is conserved in very different genomic contexts, namely in the unicellular cyanobacteria Microcystis aeruginosa PCC 7806 and Synechocystis sp. PCC 6803. Syα-fur RNA covers only part of the coding sequence of the fur orthologue sll0567, whose flanking genes encode two hypothetical proteins. Transcriptional analysis of fur and its adjacent genes in Microcystis unravels a highly compact organization of this locus involving overlapping transcripts. Maα-fur RNA spans the whole Mafur CDS and part of the flanking dnaJ and sufE sequences. In addition, Mafur seems to be part of a dicistronic operon encoding this regulator and an α-sufE RNA. These results allow new insights into the transcriptomes of two unicellular cyanobacteria and suggest that in M. aeruginosa PCC 7806, the α-fur and α-sufE RNAs might participate in a regulatory connection between the genes of the dnaJ-fur-sufE locus.

  20. Analysis of a Ferric Uptake Regulator (Fur) Mutant ofDesulfovibrio vulgaris Hildenborough

    SciTech Connect

    Bender, Kelly S.; Yen, Huei-Che Bill; Hemme, Christopher L.; Yang, Zamin K.; He, Zhili; He, Qiang; Zhou, Jizhong; Huang, Katherine H.; Alm, Eric J.; Hazen, Terry C.; Arkin, Adam P.; Wall, Judy D.

    2007-09-21

    Previous experiments examining the transcriptional profileof the anaerobe Desulfovibrio vulgaris demonstrated up-regulation of theFur regulon in response to various environmental stressors. To test theinvolvement of Fur in the growth response and transcriptional regulationof D. vulgaris, a targeted mutagenesis procedure was used for deletingthe fur gene. Growth of the resulting ?fur mutant (JW707) was notaffected by iron availability, but the mutant did exhibit increasedsensitivity to nitrite and osmotic stresses compared to the wild type.Transcriptional profiling of JW707 indicated that iron-bound Fur acts asa traditional repressor for ferrous iron uptake genes (feoAB) and othergenes containing a predicted Fur binding site within their promoter.Despite the apparent lack of siderophore biosynthesis genes within the D.vulgaris genome, a large 12-gene operon encoding orthologs to TonB andTolQR also appeared to be repressed by iron-bound Fur. While other genespredicted to be involved in iron homeostasis were unaffected by thepresence or absence of Fur, alternative expression patterns that could beinterpreted as repression or activation by iron-free Fur were observed.Both the physiological and transcriptional data implicate a globalregulatory role for Fur in the sulfate-reducing bacterium D.vulgaris.

  1. Modelling predation by transient leopard seals for an ecosystem-based management of Southern Ocean fisheries

    USGS Publications Warehouse

    Forcada, J.; Malone, D.; Royle, J. Andrew; Staniland, I.J.

    2009-01-01

    Correctly quantifying the impacts of rare apex marine predators is essential to ecosystem-based approaches to fisheries management, where harvesting must be sustainable for targeted species and their dependent predators. This requires modelling the uncertainty in such processes as predator life history, seasonal abundance and movement, size-based predation, energetic requirements, and prey vulnerability. We combined these uncertainties to evaluate the predatory impact of transient leopard seals on a community of mesopredators (seals and penguins) and their prey at South Georgia, and assess the implications for an ecosystem-based management. The mesopredators are highly dependent on Antarctic krill and icefish, which are targeted by regional fisheries. We used a state-space formulation to combine (1) a mark-recapture open-population model and individual identification data to assess seasonally variable leopard seal arrival and departure dates, numbers, and residency times; (2) a size-based bioenergetic model; and (3) a size-based prey choice model from a diet analysis. Our models indicated that prey choice and consumption reflected seasonal changes in leopard seal population size and structure, size-selective predation and prey vulnerability. A population of 104 (90-125) leopard seals, of which 64% were juveniles, consumed less than 2% of the Antarctic fur seal pup production of the area (50% of total ingested energy, IE), but ca. 12-16% of the local gentoo penguin population (20% IE). Antarctic krill (28% IE) were the only observed food of leopard seal pups and supplemented the diet of older individuals. Direct impacts on krill and fish were negligible, but the "escapement" due to leopard seal predation on fur seal pups and penguins could be significant for the mackerel icefish fishery at South Georgia. These results suggest that: (1) rare apex predators like leopard seals may control, and may depend on, populations of mesopredators dependent on prey species

  2. Modelling predation by transient leopard seals for an ecosystem-based management of Southern Ocean fisheries

    USGS Publications Warehouse

    Forcada, J.; Royle, J. Andrew; Staniland, I.J.

    2009-01-01

    Correctly quantifying the impacts of rare apex marine predators is essential to ecosystem-based approaches to fisheries management, where harvesting must be sustainable for targeted species and their dependent predators. This requires modelling the uncertainty in such processes as predator life history, seasonal abundance and movement, size-based predation, energetic requirements, and prey vulnerability. We combined these uncertainties to evaluate the predatory impact of transient leopard seals on a community of mesopredators (seals and penguins) and their prey at South Georgia, and assess the implications for an ecosystem-based management. The mesopredators are highly dependent on Antarctic krill and icefish, which are targeted by regional fisheries. We used a state-space formulation to combine (1) a mark-recapture open-population model and individual identification data to assess seasonally variable leopard seal arrival and departure dates, numbers, and residency times; (2) a size-based bioenergetic model; and (3) a size-based prey choice model from a diet analysis. Our models indicated that prey choice and consumption reflected seasonal changes in leopard seal population size and structure, size-selective predation and prey vulnerability. A population of 104 (90?125) leopard seals, of which 64% were juveniles, consumed less than 2% of the Antarctic fur seal pup production of the area (50% of total ingested energy, IE), but ca. 12?16% of the local gentoo penguin population (20% IE). Antarctic krill (28% IE) were the only observed food of leopard seal pups and supplemented the diet of older individuals. Direct impacts on krill and fish were negligible, but the ?escapement? due to leopard seal predation on fur seal pups and penguins could be significant for the mackerel icefish fishery at South Georgia. These results suggest that: (1) rare apex predators like leopard seals may control, and may depend on, populations of mesopredators dependent on prey species

  3. 50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? 23.69... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? (a) U.S. and foreign general provisions. For purposes of this section, CITES furbearers means bobcat (Lynx...

  4. 50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? 23.69... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? (a) U.S. and foreign general provisions. For purposes of this section, CITES furbearers means bobcat (Lynx...

  5. 50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? 23.69... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? (a) U.S. and foreign general provisions. For purposes of this section, CITES furbearers means bobcat (Lynx...

  6. Pressure Actuated Leaf Seals for Improved Turbine Shaft Sealing

    NASA Technical Reports Server (NTRS)

    Grondahl, Clayton

    2006-01-01

    This presentation introduces a shaft seal in which leaf seal elements are constructed from slotted shim material formed and layered into a frusto-conical assembly. Limited elastic deflection of seal leaves with increasing system pressure close large startup clearance to a small, non-contacting, steady state running clearance. At shutdown seal elements resiliently retract as differential seal pressure diminishes. Large seal clearance during startup and shutdown provides a mechanism for rub avoidance. Minimum operating clearance improves performance and non-contacting operation promises long seal life. Design features of this seal, sample calculations at differential pressures up to 2400 psid and benefit comparison with brush and labyrinth seals is documented in paper, AIAA 2005 3985, presented at the Advanced Seal Technology session of the Joint Propulsion Conference in Tucson this past July. In this presentation use of bimetallic leaf material will be discussed. Frictional heating of bimetallic leaf seals during a seal rub can relieve the rub condition to some extent with a change in seal shape. Improved leaf seal rub tolerance is expected with bimetallic material.

  7. FurA from Anabaena PCC 7120: New insights on its regulation and the interaction with DNA

    NASA Astrophysics Data System (ADS)

    Hernández, J. A.; López-Gomollón, S.; Pellicer, S.; Martín, B.; Sevilla, E.; Bes, M. T.; Peleato, M. L.; Fillat, M. F.

    2006-08-01

    Fur (ferric uptake regulator) proteins are global regulatory proteins involved in the maintenance of iron homeostasis. They recognize specific DNA sequences denoted iron boxes. It is assumed that Fur proteins act as classical repressors. Under iron-rich conditions, Fur dimers complexed with ferrous ions bind to iron boxes, preventing transcription. In addition to iron homeostasis, Fur proteins control the concerted response to oxidative and acidic stresses in heterotrophic prokaryotes. Our group studies the interaction between Fur proteins and target DNA sequences. Moreover, the regulation of FurA in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, whose genome codes for three fur homologues has been investigated. We present an overview about the different factors involved in the regulation of FurA and analyze the parameters that influence FurA-DNA interaction in the cyanobacterium Anabaena PCC 7120.

  8. Shaft seal assembly and method

    NASA Technical Reports Server (NTRS)

    Keba, John E. (Inventor)

    2007-01-01

    A pressure-actuated shaft seal assembly and associated method for controlling the flow of fluid adjacent a rotatable shaft are provided. The seal assembly includes one or more seal members that can be adjusted between open and closed positions, for example, according to the rotational speed of the shaft. For example, the seal member can be configured to be adjusted according to a radial pressure differential in a fluid that varies with the rotational speed of the shaft. In addition, in the closed position, each seal member can contact a rotatable member connected to the shaft to form a seal with the rotatable member and prevent fluid from flowing through the assembly. Thus, the seal can be closed at low speeds of operation and opened at high speeds of operation, thereby reducing the heat and wear in the seal assembly while maintaining a sufficient seal during all speeds of operation.

  9. 46. SECOND FLOOR CEILING FRAMING. The 1812 furring strips are ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    46. SECOND FLOOR CEILING FRAMING. The 1812 furring strips are still in place, fastened to the undersides of the 1755 joists. The joists frame into the bottom chords of the 1755 salvaged trusses. Note the numerals, VIII (8), N (9), X (10), and XI (11) on alternate panels of the bottom chord of the truss. Between the reinforcing plates note the numeral VII (7), added for the 1812 installation - Twelfth Street Meeting House, 20 South Twelfth Street, Philadelphia, Philadelphia County, PA

  10. Metal content in the fur of domestic and experimental animals

    SciTech Connect

    Doi, R.; Nakaya, K.; Ohno, H.; Yamashita, K.; Kobayashi, T.; Kasai, S.

    1986-08-01

    Bird feathers and animal hairs accumulate various metals, and they have been used often as analytical materials for biological monitoring of essential and pollutant metals. The usefulness of those materials, however, is relatively limited probably owing to insufficient knowledge on inter- and intra-species characteristics of metal accumulation in animals. The present report deals with the characteristics of metal accumulation in the fur of domestic and experimental animals.

  11. Comparative genomics reveals 'novel' Fur regulated sRNAs and coding genes in diverse proteobacteria.

    PubMed

    Sridhar, Jayavel; Sabarinathan, Radhakrishnan; Gunasekaran, Paramasamy; Sekar, Kanagaraj

    2013-03-10

    Ferric uptake regulator (Fur) is a transcriptional regulator controlling the expression of genes involved in iron homeostasis and plays an important role in pathogenesis. Fur-regulated sRNAs/CDSs were found to have upstream Fur Binding Sites (FBS). We have constructed a Positional Weight Matrix from 100 known FBS (19 nt) and tracked the 'Orphan' FBSs. Possible Fur regulated sRNAs and CDSs were identified by comparing their genomic locations with the 'Orphan' FBSs identified. Thirty-eight 'novel' and all known Fur regulated sRNAs in nine proteobacteria were identified. In addition, we identified high scoring FBSs in the promoter regions of the 304 CDSs and 68 of them were involved in siderophore biosynthesis, iron-transporters, two-component system, starch/sugar metabolism, sulphur/methane metabolism, etc. The present study shows that the Fur regulator controls the expression of genes involved in diverse metabolic activities and it is not limited to iron metabolism alone.

  12. Oil Saving Seal

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Driven under difficult field conditions, the Army Jeep shown went more than 22,000 miles without an oil change in a test conducted by the U.S. Army Mobility Equipment Research and Development Command. Key to this exceptionally long oil life was a set of piston ring seals made of a new synthetic rubber formula called RC-34; the seal pictured, photographed after its arduous Army trial, shows no signs of deterioration. The seal and the RC-34 material, which may soon be available for use in the family auto, were developed by Ramsey Corporation, St. Louis, Missouri, a division of TRW Automotive Worldwide. The oil in an automobile engine must be I replaced every few thousand miles not because it wears out but because it becomes contaminated. The contamination sources are gasoline and combustion gases which blow by the piston rings to mix with the oil, reducing the oil's ability to lubricate properly. Seeking to prolong oil life by eliminating "blowby," Ramsey Corporation looked for a better way to seal piston rings and used NASA technology as a departure point. The parent company TRW, under contract to NASA's Jet Propulsion Laboratory, had developed seals and bladders from a type of material called elastomers which where designed to withstand the environmental extremes of interplanetary flight. That effort formed a knowledge base for research which culminated in Ramsey's RC-34 elastomer.

  13. Identification and characterization of novel Helicobacter pylori apo-fur-regulated target genes.

    PubMed

    Carpenter, Beth M; Gilbreath, Jeremy J; Pich, Oscar Q; McKelvey, Ann M; Maynard, Ernest L; Li, Zhao-Zhang; Merrell, D Scott

    2013-12-01

    In Helicobacter pylori, the ferric uptake regulator (Fur) has evolved additional regulatory functions not seen in other bacteria; it can repress and activate different groups of genes in both its iron-bound and apo forms. Because little is understood about the process of apo-Fur repression and because only two apo-Fur-repressed genes (pfr and sodB) have previously been identified, we sought to expand our understanding of this type of regulation. Utilizing published genomic studies, we selected three potential new apo-Fur-regulated gene targets: serB, hydA, and the cytochrome c553 gene. Transcriptional analyses confirmed Fur-dependent repression of these genes in the absence of iron, as well as derepression in the absence of Fur. Binding studies showed that apo-Fur directly interacted with the suspected hydA and cytochrome c553 promoters but not that of serB, which was subsequently shown to be cotranscribed with pfr; apo-Fur-dependent regulation occurred at the pfr promoter. Alignments of apo-regulated promoter regions revealed a conserved, 6-bp consensus sequence (AAATGA). DNase I footprinting showed that this sequence lies within the protected regions of the pfr and hydA promoters. Moreover, mutation of the sequence in the pfr promoter abrogated Fur binding and DNase protection. Likewise, fluorescence anisotropy studies and binding studies with mutated consensus sequences showed that the sequence was important for apo-Fur binding to the pfr promoter. Together these studies expand the known apo-Fur regulon in H. pylori and characterize the first reported apo-Fur box sequence.

  14. Design guide for helicopter transmission seals

    NASA Technical Reports Server (NTRS)

    Hayden, T. S.; Keller, C. H., Jr.

    1974-01-01

    A detailed approach for the selection and design of seals for helicopter transmissions is presented. There are two major types of seals presently being used and they are lip type seals and mechanical type seals. Lip type seals can be divided in conventional lip seals and hydrodynamic lip seals. Conventional lip seals can be used for slow-speed, low-pressure, low-temperature sealing. Hydrodynamic lip seals although they are as pressure and temperature limited as conventional lip seals, can operate at a higher speed. Mechanical types seals are comprised of face seals and circumferential seals. Face seals are capable of high speed, high pressure, and high temperature. Circumferential seals can be used in high-speed and high-temperature applications, but will leak excessively at moderate pressures. The performance goals of transmission seals are a life that is at least equal to the scheduled overhaul interval of the gearbox component and a leakage rate of near zero.

  15. Seal system with integral detector

    DOEpatents

    Fiarman, Sidney

    1985-01-01

    There is disclosed a seal system for materials where security is of the essence, such as nuclear materials, which is tamper-indicating, which indicates changes in environmental conditions that evidence attempts to by-pass the seal, which is unique and cost effective, said seal system comprised of a seal where an optical signal is transmitted through a loop, with a detector to read said signal, and one or more additional detectors designed to detect environmental changes, these detectors being operatively associated with the seal so that detection of a break in the optical signal or detection of environmental changes will cause an observable change in the seal.

  16. Xenobiotic and Immune-Relevant Molecular Biomarkers in Harbor Seals as Proxies for Pollutant Burden and Effects.

    PubMed

    Lehnert, Kristina; Ronnenberg, Katrin; Weijs, Liesbeth; Covaci, Adrian; Das, Krishna; Hellwig, Veronika; Siebert, Ursula

    2016-01-01

    Harbor seals are exposed to increasing pressure caused by anthropogenic activities in their marine environment. Persistent organic pollutants (POPs) and trace elements are hazardous contaminants that accumulate in tissues of harbor seals. POPs and trace elements can negatively affect the immune-system and have been reported, e.g., to increase susceptibility to viral infections in seals. Biomarkers of the xenobiotic metabolism, cytokines, and heat-shock protein as cell mediators of the immune-system were established to evaluate the impact of environmental stressors on harbor seals. Harbor seals (n = 54) were captured on sandbanks in the North Sea during 2009-2012. Health assessments, including hematology, were performed, and RNAlater blood samples were taken and analyzed using quantitative polymerase chain reaction. Normalized transcript copy numbers were correlated to hematology and POP concentration in blood and trace metals in blood and fur. A significant correlation between xenobiotic markers and contaminant burden was found. Significant interrelationships between markers and POP compounds, as well as with season, weight, and hematology values, indicate that biomarkers reflect pollutant exposure and effects. A significant relationship between cortisol levels and heat-shock protein expression was observed indicating stress experienced during restraint of the seals. Interleukin-10 transcription showed significant correlations with trace elements in fur pointing toward immune regulatory effects of metal exposure. The molecular markers prove to be an important noninvasive tool that reflects contaminant exposure and the impact of anthropogenic stressors in seal species. The connection between interleukin-2, xenobiotic markers, and pollutants may indicate immune suppression in animals exposed to contaminants with subsequent susceptibility to inflammatory disease.

  17. Analysis of a ferric uptake regulator (Fur) mutant of Desulfovibrio vulgaris Hildenborough.

    PubMed

    Bender, Kelly S; Yen, Huei-Che Bill; Hemme, Christopher L; Yang, Zamin; He, Zhili; He, Qiang; Zhou, Jizhong; Huang, Katherine H; Alm, Eric J; Hazen, Terry C; Arkin, Adam P; Wall, Judy D

    2007-09-01

    Previous experiments examining the transcriptional profile of the anaerobe Desulfovibrio vulgaris demonstrated up-regulation of the Fur regulon in response to various environmental stressors. To test the involvement of Fur in the growth response and transcriptional regulation of D. vulgaris, a targeted mutagenesis procedure was used for deleting the fur gene. Growth of the resulting Deltafur mutant (JW707) was not affected by iron availability, but the mutant did exhibit increased sensitivity to nitrite and osmotic stresses compared to the wild type. Transcriptional profiling of JW707 indicated that iron-bound Fur acts as a traditional repressor for ferrous iron uptake genes (feoAB) and other genes containing a predicted Fur binding site within their promoter. Despite the apparent lack of siderophore biosynthesis genes within the D. vulgaris genome, a large 12-gene operon encoding orthologs to TonB and TolQR also appeared to be repressed by iron-bound Fur. While other genes predicted to be involved in iron homeostasis were unaffected by the presence or absence of Fur, alternative expression patterns that could be interpreted as repression or activation by iron-free Fur were observed. Both the physiological and transcriptional data implicate a global regulatory role for Fur in the sulfate-reducing bacterium D. vulgaris.

  18. Physiological and Transcriptomic Analyses to Characterize the Function of Fur and Iron Response in Shewanella oneidensis

    SciTech Connect

    Yang, Yunfeng; Harris, Daniel P; Luo, Feng; Wu, Liyou; Parsons, Andrea; Palumbo, Anthony Vito; Zhou, Jizhong

    2008-01-01

    Maintaining iron homeostasis is a key metabolic challenge for most organisms. In many bacterial species, regulation of iron homeostasis is carried out by the global transcriptional regulator Fur. Physiological examination showed that Shewanella oneidensis harboring a fur deletion mutation had deficiencies in both growth and acid tolerance response. However, the fur mutant better tolerated iron-limited environments than the wild-type strain MR-1. Transcriptomic studies comparing the fur mutant and MR-1 confirmed previous findings that iron acquisition systems were highly induced by Fur inactivation. In addition, the temporal gene expression profiling of the fur mutant in response to iron depletion and repletion suggested that a number of genes involved in energy transport were iron-responsive but Fur-independent. Further identification of Fur-independent genes was obtained by generating a gene co-expression network from temporal gene expression profiles. A group of genes is involved in heat shock and has an rpoH-binding site at their promoters, and genes related to anaerobic energy transport has a highly conserved Crp binding site at the promoters. Together, this work provides useful information for the characterization of the function of Fur and the iron response in S. oneidensis.

  19. From Peptide Aptamers to Inhibitors of FUR, Bacterial Transcriptional Regulator of Iron Homeostasis and Virulence.

    PubMed

    Mathieu, Sophie; Cissé, Cheickna; Vitale, Sylvia; Ahmadova, Aynur; Degardin, Mélissa; Pérard, Julien; Colas, Pierre; Miras, Roger; Boturyn, Didier; Covès, Jacques; Crouzy, Serge; Michaud-Soret, Isabelle

    2016-09-16

    FUR (Ferric Uptake Regulator) protein is a global transcriptional regulator that senses iron status and controls the expression of genes involved in iron homeostasis, virulence, and oxidative stress. Ubiquitous in Gram-negative bacteria and absent in eukaryotes, FUR is an attractive antivirulence target since the inactivation of the fur gene in various pathogens attenuates their virulence. The characterization of 13-aa-long anti-FUR linear peptides derived from the variable part of the anti-FUR peptide aptamers, that were previously shown to decrease pathogenic E. coli strain virulence in a fly infection model, is described herein. Modeling, docking, and experimental approaches in vitro (activity and interaction assays, mutations) and in cells (yeast two-hybrid assays) were combined to characterize the interactions of the peptides with FUR, and to understand their mechanism of inhibition. As a result, reliable structure models of two peptide-FUR complexes are given. Inhibition sites are mapped in the groove between the two FUR subunits where DNA should also bind. Another peptide behaves differently and interferes with the dimerization itself. These results define these novel small peptide inhibitors as lead compounds for inhibition of the FUR transcription factor.

  20. Expanding the Role of FurA as Essential Global Regulator in Cyanobacteria

    PubMed Central

    González, Andrés; Bes, M. Teresa; Peleato, M. Luisa; Fillat, María F.

    2016-01-01

    In the nitrogen-fixing heterocyst-forming cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA plays a global regulatory role. Failures to eliminate wild-type copies of furA gene from the polyploid genome suggest essential functions. In the present study, we developed a selectively regulated furA expression system by the replacement of furA promoter in the Anabaena sp. chromosomes with the Co2+/Zn2+ inducible coaT promoter from Synechocystis sp. PCC 6803. By removing Co2+ and Zn2+ from the medium and shutting off furA expression, we showed that FurA was absolutely required for cyanobacterial growth. RNA-seq based comparative transcriptome analyses of the furA-turning off strain and its parental wild-type in conjunction with subsequent electrophoretic mobility shift assays and semi-quantitative RT-PCR were carried out in order to identify direct transcriptional targets and unravel new biological roles of FurA. The results of such approaches led us to identify 15 novel direct iron-dependent transcriptional targets belonging to different functional categories including detoxification and defences against oxidative stress, phycobilisome degradation, chlorophyll catabolism and programmed cell death, light sensing and response, heterocyst differentiation, exopolysaccharide biosynthesis, among others. Our analyses evidence novel interactions in the complex regulatory network orchestrated by FurA in cyanobacteria. PMID:26967347