Sample records for gamma-emitting photoaffinity label

  1. delta 9-(16 alpha-/sup 125/I)iodo-19-nortestosterone: a gamma-emitting photoaffinity label for the progesterone receptor

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lamb, D.J.; Bullock, D.W.; Hoyte, R.M.

    1988-05-01

    We have synthesized 16 alpha-iodo-4,9-estradien-17 beta-ol-3-one (delta 9-16 alpha-iodo-19-nortestosterone (delta 9-INT)) labeled with 125I (delta 9-(16 alpha-125I)INT) to provide a new gamma-emitting photoaffinity ligand for the progesterone receptor that has many advantages over the currently available (3H)R5020. We have characterized the interaction of delta 9-(16 alpha-125I)INT with the rabbit uterine progesterone receptor and have demonstrated the usefulness of this compound for studies of receptor structure. The binding of 2 nM (3H)progesterone to receptor in rabbit uterine cytosol was specifically competed for by 19-nortestosterone, 16 alpha-iodo-19-nortestosterone, and delta 9-INT. Scatchard analysis demonstrated that delta 9-(16 alpha-125I)INT and (3H)progesterone estimated the samemore » number of binding sites in rabbit uterine cytosol, with a Kd for delta 9-(16 alpha-125I)INT of about 2.7 nM. The binding of delta 9-(16 alpha-125I)INT was inhibited by both progesterone and R5020, whereas testosterone, estradiol, and 5 alpha-dihydrotestosterone were ineffective. In cytosol, delta 9-(16 alpha-125I)INT covalently labeled the same mol wt receptor forms as (3H)R5020. Although the efficiency of cross-linking was similar for (3H)R5020 (3%) and delta 9-(16 alpha-125I)INT (4%), the radioactivity was 10-fold greater due to the higher specific activity of delta 9-(16 alpha-125I)INT and the lack of sample quench. The use of delta 9-(16 alpha-125I)INT greatly increases the sensitivity and efficiency of the photoaffinity labeling technique; it will provide a valuable tool for further studies of the progesterone receptor, allowing the detection of receptor in dilute cytosol after gel electrophoresis under denaturing conditions.« less

  2. Photoaffinity-labeled Cytokinins

    PubMed Central

    Theiler, Jane B.; Leonard, Nelson J.; Schmitz, Ruth Y.; Skoog, Folke

    1976-01-01

    Two new azidopurine derivatives, 2-azido-N6-(Δ2-isopentenyl)adenine and 2-azido-N6-benzyladenine, have been synthesized as potential photoaffinity labels for probing cytokinin-binding sites. The preparation and the biological activity of these compounds are described. PMID:16659772

  3. Photoaffinity Labeling Studies on a Promoter of Dendritic Spine Formation

    NASA Astrophysics Data System (ADS)

    Sibucao, Kevin Carlo Abril

    The small molecule BTA-EG4 has been shown to be a promoter of dendritic spine formation. The mechanism behind this phenomenon, however, is not well understood. The work in this dissertation is motivated by this gap in knowledge. The first part of this dissertation focuses on photoaffinity labeling studies to identify the cellular targets of BTA-EG4. Chapter 1 provides a summary of Alzheimer's disease, the rational design of BTA-EG 4, and methods to determine targets of small molecules. In Chapter 2, the synthesis of a BTA-EG4-based photoaffinity labeling probe and photodegradation studies are presented. Kinetic studies demonstrate that the probe photolyzes rapidly under UV light. In Chapter 3, photoaffinity labeling studies and subsequent protein identification experiments are reported. Competition experiments with the photoaffinity labeling probe and BTA-EG4 demonstrate that the probe labels a 55-kDa protein specifically. Tandem mass spectrometry revealed that the 55-kDa protein is the actin binding protein fascin 1. The second part of this dissertation focuses on the major protein identified from photoaffinity labeling studies, fascin 1. Chapter 4 provides a brief survey of the structure and function of fascin 1. In Chapter 5, characterizations of the interaction between BTA-EG4 and fascin 1 are reported. Isothermal titration calorimetry confirms the physical binding between fascin 1 and BTA-EG6, a BTA-EG4 analog. Slow speed sedimentation assays reveal that BTA-EG4 does not affect the actin-bundling activity of fascin 1. However, GST pull-down experiments show that BTA-EG4 inhibits the binding of fascin 1 with the GTPase Rab35. In addition, this work demonstrates that BTA-EG4 may be mechanistically distinct from the known fascin inhibitor G2.

  4. 2-Aryl-5-carboxytetrazole as a New Photoaffinity Label for Drug Target Identification

    PubMed Central

    2016-01-01

    Photoaffinity labels are powerful tools for dissecting ligand–protein interactions, and they have a broad utility in medicinal chemistry and drug discovery. Traditional photoaffinity labels work through nonspecific C–H/X–H bond insertion reactions with the protein of interest by the highly reactive photogenerated intermediate. Herein, we report a new photoaffinity label, 2-aryl-5-carboxytetrazole (ACT), that interacts with the target protein via a unique mechanism in which the photogenerated carboxynitrile imine reacts with a proximal nucleophile near the target active site. In two distinct case studies, we demonstrate that the attachment of ACT to a ligand does not significantly alter the binding affinity and specificity of the parent drug. Compared with diazirine and benzophenone, two commonly used photoaffinity labels, in two case studies ACT showed higher photo-cross-linking yields toward their protein targets in vitro based on mass spectrometry analysis. In the in situ target identification studies, ACT successfully captured the desired targets with an efficiency comparable to the diazirine. We expect that further development of this class of photoaffinity labels will lead to a broad range of applications across target identification, and validation and elucidation of the binding site in drug discovery. PMID:27740749

  5. 2-Aryl-5-carboxytetrazole as a New Photoaffinity Label for Drug Target Identification.

    PubMed

    Herner, András; Marjanovic, Jasmina; Lewandowski, Tracey M; Marin, Violeta; Patterson, Melanie; Miesbauer, Laura; Ready, Damien; Williams, Jon; Vasudevan, Anil; Lin, Qing

    2016-11-09

    Photoaffinity labels are powerful tools for dissecting ligand-protein interactions, and they have a broad utility in medicinal chemistry and drug discovery. Traditional photoaffinity labels work through nonspecific C-H/X-H bond insertion reactions with the protein of interest by the highly reactive photogenerated intermediate. Herein, we report a new photoaffinity label, 2-aryl-5-carboxytetrazole (ACT), that interacts with the target protein via a unique mechanism in which the photogenerated carboxynitrile imine reacts with a proximal nucleophile near the target active site. In two distinct case studies, we demonstrate that the attachment of ACT to a ligand does not significantly alter the binding affinity and specificity of the parent drug. Compared with diazirine and benzophenone, two commonly used photoaffinity labels, in two case studies ACT showed higher photo-cross-linking yields toward their protein targets in vitro based on mass spectrometry analysis. In the in situ target identification studies, ACT successfully captured the desired targets with an efficiency comparable to the diazirine. We expect that further development of this class of photoaffinity labels will lead to a broad range of applications across target identification, and validation and elucidation of the binding site in drug discovery.

  6. Mapping of the acetylcholine binding site of the nicotinic acetylcholine receptor: ( sup 3 H)nicotine as an agonist photoaffinity label

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Middleton, R.E.; Cohen, J.B.

    1991-07-16

    The agonist ({sup 3}H)nicotine was used as a photoaffinity label for the acetylcholine binding sties on the Torpedo nicotinic acetylcholine receptor (AChR). ({sup 3}H)Nicotine binds at equilibrium with K{sub eq} = 0.6 {mu}M to the agonist binding sites. Irradiation with 254-nm light of AChR-rich membranes equilibrated with ({sup 3}H)nicotine resulted in covalent incorporation into the {alpha}- and {gamma}-subunits, which was inhibited by agonists and competitive antagonists but not by noncompetitive antagonists. Inhibition of labeling by d-tubocurarine demonstrated that the {alpha}-subunit was labeled via both agonist sites but the {gamma}-subunit was labeled only via the site that binds d-tubocurarine with highmore » affinity. Chymotryptic digestion of the {alpha}-subunit confirmed that Try-198 was the principal amino acid labeled by ({sup 3}H)nicotine. This confirmation required a novel radiosequencing strategy employing o-phthalaldehyde ({sup 3}H)Nicotine, which is the first photoaffinity agonist used, labels primarily Tyr-198 in contrast to competitive antagonist affinity labels, which label primarily Tyr-190 and Cys-192/Cys-193.« less

  7. Photoaffinity labelling of the ATP-binding site of the epidermal growth factor-dependent protein kinase.

    PubMed

    Kudlow, J E; Leung, Y

    1984-06-15

    Epidermal growth factor (EGF), after binding to its receptor, activates a tyrosine-specific protein kinase which phosphorylates several substrates, including the EGF receptor itself. The effects of a photoaffinity analogue of ATP, 3'-O-(3-[N-(4-azido-2-nitrophenyl)amino]propionyl)adenosine 5'-triphosphate (arylazido-beta-alanyl-ATP) on the EGF-dependent protein kinase in A431 human tumour cell plasma membrane vesicles was investigated. This analogue was capable of inactivating the EGF-receptor kinase in a photodependent manner. Partial inactivation occurred at an analogue concentration of 1 microM and complete inactivation occurred at 10 microM when a 2 min light exposure was used. Arylazido-beta-alanine at 100 microM and ATP at 100 microM were incapable of inactivating the enzyme with 2 min of light exposure. The photodependent inactivation of the enzyme by the analogue could be partially blocked by 20 mM-ATP and more effectively blocked by either 20 mM-adenosine 5'-[beta gamma-imido]triphosphate or 20 mM-guanosine 5'-[beta gamma-imido]triphosphate, indicating nucleotide-binding site specificity. Arylazido-beta-alanyl-[alpha-32P]ATP was capable of labelling membrane proteins in a photodependent manner. Numerous proteins were labelled, the most prominent of which ran with an apparent Mr of 53000 on polyacrylamide-gel electrophoresis. A band of minor intensity was seen of Mr corresponding to the EGF receptor (170000). Immunoprecipitation of affinity-labelled and solubilized membranes with an anti-(EGF receptor) monoclonal antibody demonstrated that the Mr 170000 receptor protein was photoaffinity labelled by the analogue. The Mr 53000 peptide was not specifically bound by the anti-receptor antibody. The affinity labelling of the receptor was not enhanced by EGF, suggesting that EGF stimulation of the kinase activity does not result from changes in the affinity of the kinase for ATP. These studies demonstrate that arylazido-beta-alanyl-ATP interacts with the ATP

  8. Photoaffinity-labeling and fluorescence-distribution studies of gonadotropin-releasing hormone receptors in ovarian granulosa cells.

    PubMed Central

    Hazum, E; Nimrod, A

    1982-01-01

    Photoaffinity labeling of rat ovarian granulosa cells and membrane preparations with a bioactive photoaffinity derivative of gonadotropin-releasing hormone resulted in identification of two specific components with apparent molecular weights of 60,000 and 54,000. Fluorescent visualization of gonadotropin-releasing hormone receptors in these cells, by using a bioactive rhodamine derivative of the hormone, indicated that the fluorescently labeled receptors were initially distributed uniformly on the cell surface and then formed patches that subsequently internalized (at 37 degrees C) into endocytic vesicles. These processes were dependent on specific binding sites for the rhodamine-labeled peptide on the granulosa cells. These studies may provide an experimental basis for understanding the molecular events involved in the action of the hormone in the ovary. Images PMID:6281784

  9. Derivativation of the human erythrocyte glucose transporter using a novel forskolin photoaffinity label

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wadzinski, B.; Shanahan, M.; Ruoho, A.

    1987-05-01

    An iodinated photoaffinity label for the glucose transporter, 3-iodo-4-azidophenethylamido-7-0-succinyldeacetyl-forskolin (IAPS-Fsk), has been synthesized, purified, and characterized. The K/sub i/ for inhibition of 3-0-methylglucose transport by TAPS-Fsk in human erythrocytes was found to be 0.1 uM. The carrier-free radioiodinated label has been shown to be a highly specific photoaffinity label for the human erythrocyte glucose transporter. Photolysis of erythrocyte membranes with 1-10 nM (I-125)IAPS-Fsk and analysis by SDS-PAGE showed specific derivatization of a broad band with an apparent molecular weight of 40-70 kDa. Photoincorporation using 2 nM (I-125)IAPS-Fsk was protected with D-glucose, cytochalasin B, and forskolin. No protection was observed withmore » L-glucose. Endo-B-galactosidase digestion and trypsinization of (I-125)IAPS-Fsk labelled erythrocytes reduced the specifically radiolabelled transporter to 40 kDa and 18 kDa respectively. (I-125)-IAPS-Fsk will be used to study the structural aspects of the glucose transporter.« less

  10. Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin

    NASA Technical Reports Server (NTRS)

    Hicks, G. R.; Rayle, D. L.; Jones, A. M.; Lomax, T. L.

    1989-01-01

    Plasma membrane vesicles were isolated from zucchini (Cucurbita pepo) hypocotyl tissue by aqueous phase partitioning and assessed for homogeneity by the use of membrane-specific enzyme assays. The highly pure (ca. 95%) plasma membrane vesicles maintained a pH differential across the membrane and accumulated a tritiated azido analogue of 3-indoleacetic acid (IAA), 5-azido-[7-3H]IAA ([3H]N3IAA), in a manner similar to the accumulation of [3H]IAA. The association of the [3H]N3IAA with membrane vesicles was saturable and subject to competition by IAA and auxin analogues. Auxin-binding proteins were photoaffinity labeled by addition of [3H]N3IAA to plasma membrane vesicles prior to exposure to UV light (15 sec; 300 nm) and detected by subsequent NaDodSO4/PAGE and fluorography. When the reaction temperature was lowered to -196 degrees C, high-specific-activity labeling of a 40-kDa and a 42-kDa polypeptide was observed. Triton X-100 (0.1%) increased the specific activity of labeling and reduced the background, which suggests that the labeled polypeptides are intrinsic membrane proteins. The labeled polypeptides are of low abundance, as expected for auxin receptors. Further, the addition of IAA and auxin analogues to the photoaffinity reaction mixture resulted in reduced labeling that was qualitatively similar to their effects on the accumulation of radiolabeled IAA in membrane vesicles. Collectively, these results suggest that the radiolabeled polypeptides are auxin receptors. The covalent nature of the label should facilitate purification and further characterization of the receptors.

  11. Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin.

    PubMed

    Hicks, G R; Rayle, D L; Jones, A M; Lomax, T L

    1989-07-01

    Plasma membrane vesicles were isolated from zucchini (Cucurbita pepo) hypocotyl tissue by aqueous phase partitioning and assessed for homogeneity by the use of membrane-specific enzyme assays. The highly pure (ca. 95%) plasma membrane vesicles maintained a pH differential across the membrane and accumulated a tritiated azido analogue of 3-indoleacetic acid (IAA), 5-azido-[7-3H]IAA ([3H]N3IAA), in a manner similar to the accumulation of [3H]IAA. The association of the [3H]N3IAA with membrane vesicles was saturable and subject to competition by IAA and auxin analogues. Auxin-binding proteins were photoaffinity labeled by addition of [3H]N3IAA to plasma membrane vesicles prior to exposure to UV light (15 sec; 300 nm) and detected by subsequent NaDodSO4/PAGE and fluorography. When the reaction temperature was lowered to -196 degrees C, high-specific-activity labeling of a 40-kDa and a 42-kDa polypeptide was observed. Triton X-100 (0.1%) increased the specific activity of labeling and reduced the background, which suggests that the labeled polypeptides are intrinsic membrane proteins. The labeled polypeptides are of low abundance, as expected for auxin receptors. Further, the addition of IAA and auxin analogues to the photoaffinity reaction mixture resulted in reduced labeling that was qualitatively similar to their effects on the accumulation of radiolabeled IAA in membrane vesicles. Collectively, these results suggest that the radiolabeled polypeptides are auxin receptors. The covalent nature of the label should facilitate purification and further characterization of the receptors.

  12. Photoaffinity labeling of regulatory subunits of protein kinase A in cardiac cell fractions of rats

    NASA Technical Reports Server (NTRS)

    Mednieks, M. I.; Popova, I.; Grindeland, R. E.

    1992-01-01

    Photoaffinity labeling in heart tissue of rats flown on Cosmos 2044 was used to measure the regulatory (R) subunits of adenosine monophosphate-dependent protein kinase. A significant decrease of RII subunits in the particulate cell fraction extract (S2; P less than 0.05 in all cases) was observed when extracts of tissue samples from vivarium controls were compared with those from flight animals. Photoaffinity labeling of the soluble fraction (S1) was observed to be unaffected by spaceflight or any of the simulation conditions. Proteins of the S2 fraction constitute a minor (less than 10 percent) component of the total, whereas the S1 fraction contained most of the cell proteins. Changes in a relatively minor aspect of adenosine monophosphate-mediated reactions are considered to be representative of a metabolic effect.

  13. Photoaffinity labeling in target- and binding-site identification

    PubMed Central

    Smith, Ewan; Collins, Ian

    2015-01-01

    Photoaffinity labeling (PAL) using a chemical probe to covalently bind its target in response to activation by light has become a frequently used tool in drug discovery for identifying new drug targets and molecular interactions, and for probing the location and structure of binding sites. Methods to identify the specific target proteins of hit molecules from phenotypic screens are highly valuable in early drug discovery. In this review, we summarize the principles of PAL including probe design and experimental techniques for in vitro and live cell investigations. We emphasize the need to optimize and validate probes and highlight examples of the successful application of PAL across multiple disease areas. PMID:25686004

  14. Photoaffinity labelling of the active site of the rat glutathione transferases 3-3 and 1-1 and human glutathione transferase A1-1.

    PubMed

    Cooke, R J; Björnestedt, R; Douglas, K T; McKie, J H; King, M D; Coles, B; Ketterer, B; Mannervik, B

    1994-09-01

    The glutathione transferases (GSTs) form a group of enzymes responsible for a wide range of molecular detoxications. The photoaffinity label S-(2-nitro-4-azidophenyl)glutathione was used to study the hydrophobic region of the active site of the rat liver GST 1-1 and 2-2 isoenzymes (class Alpha) as well as the rat class-Mu GST 3-3. Photoaffinity labelling was carried out using a version of S-(2-nitro-4-azidophenyl)glutathione tritiated in the arylazido ring. The labelling occurred with higher levels of radioisotope incorporation for the Mu than the Alpha families. Taking rat GST 3-3, 1.18 (+/- 0.05) mol of radiolabel from S-(2-nitro-4-azidophenyl)glutathione was incorporated per mol of dimeric enzyme, which could be blocked by the presence of the strong competitive inhibitor, S-tritylglutathione (Ki = 1.4 x 10(-7) M). Radiolabelling of the protein paralleled the loss of enzyme activity. Photoaffinity labelling by tritiated S-(2-nitro-4-azidophenyl)glutathione on a preparative scale (in the presence and absence of S-tritylglutathione) followed by tryptic digestion and purification of the labelled peptides indicated that GST 3-3 was specifically photolabelled; the labelled peptides were sequenced. Similarly, preparative photoaffinity labelling by S-(2-nitro-4-azidophenyl)glutathione of the rat liver 1-1 isoenzyme, the human GST A1-1 and the human-rat chimaeric GST, H1R1/1, was carried out with subsequent sequencing of radiolabelled h.p.l.c.-purified tryptic peptides. The results were interpreted by means of molecular-graphics analysis to locate photoaffinity-labelled peptides using the X-ray-crystallographic co-ordinates of rat GST 3-3 and human GST A1-1. The molecular-graphical analysis indicated that the labelled peptides are located within the immediate vicinity of the region occupied by S-substituted glutathione derivatives bound in the active-site cavity of the GSTs investigated.

  15. Photoaffinity labelling of MSH receptors on Anolis melanophores: effects of catecholamines, calcium and forskolin.

    PubMed

    Eberle, A N; Girard, J

    1985-01-01

    Photoaffinity labelling of MSH receptors on Anolis melanophores was used as a tool for studying the effects of catecholamines, calcium and forskolin on hormone-receptor interaction and receptor-adenylate cyclase coupling. Covalent attachment of photoreactive alpha-MSH to its receptor was suppressed in calcium-free buffer but was hardly influenced by catecholamines or forskolin. The longlasting signal generated by the covalent MSH-receptor complex was readily and reversibly abolished by adrenaline, noradrenaline, dopamine or clonidine or by the absence of calcium. The suppression of pigment dispersion by catecholamines was blocked by the simultaneous presence of yohimbine but not prazosin, indicating that the catecholamines antagonize the alpha-MSH signal by inhibitory action on the adenylate cyclase system through an alpha-2 receptor. Forskolin, which stimulates melanophores by direct action on the catalytic unit of the adenylate cyclase and at about the same speed as alpha-MSH, produced a slower and weaker response in the presence of noradrenaline. If MSH receptors were covalently labelled and then exposed to noradrenaline, the characteristics of the forskolin-induced response were identical to those of unlabelled cells that had not been exposed to noradrenaline. This may point to a partial restoration of receptor-adenylate cyclase coupling by forskolin. The results show that the longlasting stimulation of Anolis melanophores by photoaffinity labelling proceeds via a permanently stimulated adenylate-cyclase system whose coupling to the receptor depends on calcium and is abolished by alpha-2 receptor agonists. Calcium is also essential for hormone-receptor binding.

  16. Interspecies conservation of retinal guanosine 5'-triphosphatase. Characterization by photoaffinity labelling and tryptic-peptide mapping.

    PubMed Central

    McMurray, M M; Hansen, J S; Haley, B E; Takemoto, D J; Takemoto, L J

    1985-01-01

    Light-activated hydrolysis of cyclic GMP is achieved through the photoexcitation of rhodopsin, a process which then triggers the replacement of GDP for GTP by a retinal guanosine 5'-triphosphatase referred to as 'transducin'. The transducin-GTP complex then switches on the phosphodiesterase [Fung, Hurley & Stryer (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 152-156]. The bovine transducin consists of an alpha-subunit (39000 Mr), which is a GTP-binding component, together with a beta-(37000 Mr) and a gamma-subunit (10000 Mr). We have purified retinal transducin from cow, pig, chick and frog. The enzyme specific activities and sodium dodecyl sulphate/polyacrylamide-gel-electrophoretic profiles indicate that this enzyme is similar in all species except the frog. Whereas the bovine, pig and chick transducins consist of major 37000- and 39000-Mr components, that of the frog consists of a single 75000-Mr component. Labelling of the GTP-binding components with the photoaffinity label 8-azidoguanosine [gamma-32P]triphosphate demonstrated that the 37000-Mr components of the cow, pig and chick and the 75000-Mr component of the frog were major GTP-binding components. In addition, peptide maps of radioiodinated tryptic peptides indicate that the frog 75000-Mr protein is highly related to the pig transducin. These results demonstrate evolutionary conservation of retinal transducin and the presence of a higher-Mr, but nonetheless highly conserved form, of transducin in the frog. The relationship of this component to the recently reported rod-outer-segment inhibitor protein [Yamazaki, Stein, Chernoff & Bitensky (1983) J. Biol. Chem. 258, 8188-8194] is discussed. Images Fig. 2. Fig. 3. Fig. 4. Fig. 5. PMID:2983663

  17. Photoaffinity labeling of an herbicide receptor protein in chloroplast membranes

    PubMed Central

    Pfister, Klaus; Steinback, Katherine E.; Gardner, Gary; Arntzen, Charles J.

    1981-01-01

    2-Azido-4-ethylamino-6-isopropylamino-s-triazine (azido-atrazine) inhibits photosynthetic electron transport at a site identical to that affected by atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine). The latter is a well-characterized inhibitor of photosystem II reactions. Azido-atrazine was used as a photoaffinity label to identify the herbicide receptor protein; UV irradiation of chloroplast thylakoids in the presence of azido[14C]atrazine resulted in the covalent attachment of radioactive inhibitor to thylakoid membranes isolated from pea seedlings and from a triazine-susceptible biotype of the weed Amaranthus hybridus. No covalent binding of azido-atrazine was observed for thylakoid membranes isolated from a naturally occurring triazine-resistant biotype of A. hybridus. Analysis of thylakoid polypeptides from both the susceptible and resistant A. hybridus biotypes by sodium dodecyl sulfate/polyacrylamide gel electrophoresis, followed by fluorography to locate 14C label, demonstrated specific association of the azido[14C]atrazine with polypeptides of the 34- to 32-kilodalton size class in susceptible but not in resistant membranes. Images PMID:16592984

  18. Characterization of mammalian glucose transport proteins using photoaffinity labeling techniques

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wadzinski, B.E.

    1989-01-01

    A carrier-free radioiodinated phenylazide derivative of forskolin, 3-iodo-4-azidophenethylamido-7-O-succinyl-deacetyl-forskolin (({sup 125}I)IAPS-forskolin), has been shown to be a highly selective photoaffinity probe for the human erythrocyte glucose transported and the glucose transport proteins found in several mammalian tissues and cultured cells where the glucose transport protein is present at a low concentration. The photoincorporation of ({sup 125}I)IAPS-forskolin into these glucose transporters was blocked by D- (but not L-) glucose, cytochalasin B, and forskolin. In addition to labeling the mammalian glucose transport proteins, ({sup 125}I)IAPS-forskolin also labeled the L-arabinose transporter from E. coli. In muscle and adipose tissues, glucose transport is markedly increasedmore » in response to insulin. ({sup 125}I)IAPS-forskolin was shown to selectivity tag the glucose transporter in membranes derived from these cells. In addition, the covalent derivatization of the transport protein in subcellular fractions of the adipocyte has provided a means to study the hormonal regulation of glucose transport. ({sup 125}I)IAPS-forskolin has also been used to label the purified human erythrocyte glucose transporter. The site of insertion has therefore been localized by analysis of the radiolabeled peptides which were produced following chemical and proteolytic digestion of the labeled transport protein.« less

  19. Direct photoaffinity labeling of an allosteric site on subunit protein M1 of mouse ribonucleotide reductase by dTTP.

    PubMed Central

    Eriksson, S; Caras, I W; Martin, D W

    1982-01-01

    The protein M1 subunit of ribonucleotide reductase contains at least two allosteric nucleotide binding sites that control the capacity of the enzyme to reduce ribonucleotides to the deoxyribonucleotides required for DNA synthesis. Direct photoaffinity labeling of partially purified protein M1 from mouse T-lymphoma (S49) cells was observed after UV irradiation in the presence of dTTP at 0 degrees C. The relative molar incorporation of nucleotide per subunit was 4-8%. Competition experiments showed that the dTTP was bound to an allosteric domain genetically and kinetically defined as the substrate specificity site of the enzyme. An altered protein M1 isolated from a thymidine-resistant mutant cell line showed significantly decreased photoincorporation of dTTP, consistent with the fact that its CDP reductase activity is resistant to feedback inhibition by dTTP. Specific photolabeling of several other proteins with pyrimidine and purine nucleotides was also found, indicating the general usefulness of direct photoaffinity labeling in the study of enzymes involved in nucleotide and nucleic acid metabolism. Images PMID:7033963

  20. Modification of adenylate cyclase by photoaffinity analogs of forskolin

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ho, L.T.; Nie, Z.M.; Mende, T.J.

    1989-01-01

    Photoaffinity labeling analogs of the adenylate cyclase activator forskolin (PF) have been synthesized, purified and tested for their effect on preparations of membrane-bound, Lubrol solubilized and forskolin affinity-purified adenylate cyclase (AC). All analogs of forskolin significantly activated AC. However, in the presence of 0.1 to 0.3 microM forskolin, the less active forskolin photoaffinity probes at 100 microM caused inhibition. This inhibition was dose-dependent for PF, suggesting that PF may complete with F for the same binding site(s). After cross-linking (125I)PF-M to either membrane or Lubrol-solubilized AC preparations by photolysis, a radiolabeled 100-110 kDa protein band was observed after autoradiography followingmore » SDS-PAGE. F at 100 microM blocked the photoradiolabeling of this protein. Radioiodination of forskolin-affinity purified AC showed several protein bands on autoradiogram, however, only one band (Mr = 100-110 kDa) was specifically labeled by (125I)PF-M following photolysis. The photoaffinity-labeled protein of 100-110 kDa of AC preparation of rat adipocyte may be the catalytic unit of adenylate cyclase of rat adipocyte itself as supported by the facts that (a) no other AC-regulatory proteins are known to be of this size, (b) the catalytic unit of bovine brain enzyme is in the same range and (c) this PF specifically stimulates AC activity when assayed alone, and weekly inhibits forskolin-activation of cyclase. These studies indicate that radiolabeled PF probes may be useful for photolabeling and detecting the catalytic unit of adenylate cyclase.« less

  1. Photoaffinity Labeling of Ras Converting Enzyme using Peptide Substrates that Incorporate Benzoylphenylalanine (Bpa) Residues: Improved Labeling and Structural Implications

    PubMed Central

    Kyro, Kelly; Manandhar, Surya P.; Mullen, Daniel; Schmidt, Walter K.; Distefano, Mark D.

    2012-01-01

    Rce1p catalyzes the proteolytic trimming of C-terminal tripeptides from isoprenylated proteins containing CAAX-box sequences. Because Rce1p processing is a necessary component in the Ras pathway of oncogenic signal transduction, Rce1p holds promise as a potential target for therapeutic intervention. However, its mechanism of proteolysis and active site have yet to be defined. Here, we describe synthetic peptide analogues that mimic the natural lipidated Rce1p substrate and incorporate photolabile groups for photoaffinity-labeling applications. These photoactive peptides are designed to crosslink to residues in or near the Rce1p active site. By incorporating the photoactive group via p-benzoyl-L-phenylalanine (Bpa) residues directly into the peptide substrate sequence, the labeling efficiency was substantially increased relative to a previously-synthesized compound. Incorporation of biotin on the N-terminus of the peptides permitted photolabeled Rce1p to be isolated via streptavidin affinity capture. Our findings further suggest that residues outside the CAAX-box sequence are in contact with Rce1p, which has implications for future inhibitor design. PMID:22079863

  2. Both α and β Subunits of Human Choriogonadotropin Photoaffinity Label the Hormone Receptor

    NASA Astrophysics Data System (ADS)

    Ji, Inhae; Ji, Tae H.

    1981-09-01

    It has been shown that a photoactivable derivative of human choriogonadotropin (hCG) labels the lutropin receptor on porcine granulosa cells [Ji, I. & Ji, T. H. (1980) Proc. Natl. Acad. Sci. USA 77, 7167-7170]. In an attempt to identify which of the hCG subunits labeled the receptor, three sets of different hCG derivatives were prepared. In the first set, hCG was coupled to the N-hydroxysuccinimide ester of 4-azidobenzoylglycine and radioiodinated. In the second set, only one of the subunits was radioiodinated, but both subunits were allowed to react with the reagent. In the third set, both the reagent and [125I]iodine were coupled to only one of the subunits. The binding activity of each hormone derivative was comparable to that of 125I-labeled hCG. After binding of these hormone derivatives to the granulosa cell surface, they were photolyzed. After solubilization, autoradiographs of sodium dodecyl sulfate/polyacrylamide gels of each sample revealed a number of labeled bands; the hCG derivatives containing 125I-labeled alpha subunit produced four bands (molecular weights 120,000 +/- 6,000, 96,000 +/- 5,000, 76,000 +/- 4,000, and 73,000 +/- 4,000) and those containing 125I-labeled beta subunit produced three bands (molecular weights 106,000 +/- 6,000, 88,000 +/- 5,000, and 83,000 +/- 4,000). Results were the same when the hormone-receptor complexes were solubilized in 0.5% Triton X-100 and then photolyzed or when the hormone was derivatized with a family of reagents having arms of various lengths. We conclude that both the alpha subunit and the beta subunit of hCG photoaffinity labeled certain membrane polypeptides and that these polypeptides are related to the hormone receptor.

  3. Juvenile hormone-binding proteins of Melanoplus bivittatus identified by EFDA photoaffinity labeling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Winder, B.S.

    1988-01-01

    Proteins that bind juvenile hormone in the hemolymph and fat body of the grasshopper, Melanoplus bivittatus were identified by photoaffinity labeling with radiolabeled epoxyfarnesyl diazoacetate ({sup 3}H-EFDA), and were characterized by electrophoretic analysis. A protocol was developed which allowed detection of {sup 3}H-EFDA that was covalently linked to proteins upon exposure to ultraviolet light at 254 nm. Quantification of protein-linked {sup 3}H-EFDA by liquid scintillation spectrometry took advantage of the differential solubility of unlinked {sup 3}H-EFDA in toluene alone, and of the protein-linked {sup 3}H-EFDA in toluene plus the detergent, Triton X-100. Competition between EFDA and juvenile hormone (JH) formore » binding to JH-specific binding sites was measured by hydroxyapatite protein binding assays in the presence of radiolabeled JH or EFDA and competing non-radiolabeled hormone. The protein-linked EFDA was detected on fluorograms of SDS or nondenaturing polyacrylamide gels (PAGE), and by liquid scintillation spectrometry of membranes to which the proteins had been electrophoretically transferred. Proteins which specifically bound JH were identified by photolabeling proteins in the presence and absence of nonlabeled JH-III.« less

  4. Photoaffinity labelling and solubilization on the central 5-HT1A receptor binding site.

    PubMed

    Gozlan, H; Emerit, M B; el Mestikawy, S; Cossery, J M; Marquet, A; Besselievre, R; Hamon, M

    1987-01-01

    Two complementary approaches, covalent labelling and solubilization, have been used to study the biochemical properties of the central 5-HT1A receptor binding site. We have first designed a photoaffinity ligand containing the structure of 8-OH-DPAT, a potent and specific agonist of 5-HT1A sites. Thus, 8-methoxy-2[N-n-propyl,N-3-(2-nitro-4-azido-phenyl)- aminopropyl]aminotetralin or 8-methoxy-3'-NAP-amino-PAT, was found to displace, in the dark, [3H]8-OH-DPAT from 5-HT1A sites in rat hippocampal membranes with an IC50 of 6.6 nM. Under two cumulative UV irradiations (366 nm, for 20 min at 4 degrees C), 8-methoxy-3-'-NAP-amino-PAT (30 nM) blocked irreversibly 55-60% of 5-HT1A binding sites. This blockade was specific of 5-HT1A sites since the other serotoninergic sites, 5-HT1B, 5-HT2 and also the presynaptic 5-HT3 sites were not affected by the treatment. In addition, the binding of [3H]Spiperone and [3H]7-OH-DPAT to striatal dopamine sites remained unchanged under similar photolysis conditions. The tritiated derivative of the photoaffinity ligand (92 Ci/mmol) was then synthesized for the identification of the covalently bound protein(s). SDS-PAGE of solubilized membranes irradiated in the presence of 20 nM 3H-8-methoxy-3'-NAP-amino-PAT allowed the detection of a 63 kD protein whose labelling appeared specific. Thus, 3H-incorporation into the 63 kD band could be prevented by microM concentrations of 5-HT, 8-OH-DPAT and other selective 5-HT1A ligands such as isapirone. In contrast, the 5-HT2 antagonist ketanserin, norepinephrine and dopamine-related ligands (including 7-OH-DPAT) were ineffective. Direct solubilization of 5-HT1A receptor binding sites was also attempted from rat hippocampal membranes. The best results were obtained using CHAPS (10 mM) plus NaCl (0.2 M), which led to 50% recovery of 5-HT1A sites in the 100,000 g supernatant. The pharmacological properties and sensitivity to N-ethyl-maleimide and GppNHp of soluble sites appeared near identical to those of

  5. Identification of amino acids in the N-terminal SH2 domain of phospholipase C gamma 1 important in the interaction with epidermal growth factor receptor.

    PubMed

    Gergel, J R; McNamara, D J; Dobrusin, E M; Zhu, G; Saltiel, A R; Miller, W T

    1994-12-13

    Photoaffinity labeling and site-directed mutagenesis have been used to identify amino acid residues of the phospholipase C gamma 1 (PLC gamma 1) N-terminal SH2 domain involved in recognition of the activated epidermal growth factor receptor (EGFR). The photoactive amino acid p-benzoylphenylalanine (Bpa) was incorporated into phosphotyrosine-containing peptides derived from EGFR autophosphorylation sites Tyr992 and Tyr1068. Irradiation of these labels in the presence of SH2 domains showed cross-linking which was time-dependent and specific; labeling was inhibited with non-Bpa-containing peptides from EGFR in molar excess. The phosphotyrosine residue on the peptides was important for SH2 recognition, as dephosphorylated peptides did not cross-link. Radiolabeled peptides were used to identify sites of cross-linking to the N-terminal SH2 of PLC gamma 1. Bpa peptide-SH2 complexes were digested with trypsin, and radioactive fragments were purified by HPLC and analyzed by Edman sequencing. These experiments showed Arg562 and an additional site in the alpha A-beta B region of the SH2 domain, most likely Glu587, to be labeled by the Tyr992-derived peptide. Similar analysis of the reaction with the Tyr1068-derived photoaffinity label identified Leu653 as the cross-linked site. Mutation of the neighboring residues of Glu587 decreased photo-cross-linking, emphasizing the importance of this region of the molecule for recognition. These results are consistent with evidence from the v-Src crystal structure and implicate the loop spanning residues Gln640-Ser654 of PLC gamma 1 in specific recognition of phosphopeptides.

  6. Forskolin photoaffinity labels with specificity for adenylyl cyclase and the glucose transporter

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Morris, D.I.; Robbins, J.D.; Ruoho, A.E.

    1991-07-15

    Two photolabels, N-(3-(4-azido-3-125I-phenyl)-propionamide)-6- aminoethylcarbamylforskolin(125I-6-AIPP-Fsk) and N-(3-(4-azido-3-125I-phenyl)propionamide)-7-aminoethylcarbamyl-7- desacetylforskolin (125I-7-AIPP-Fsk) were synthesized with specific activities of 2200 Ci/mmol and used to label adenylyl cyclase and the glucose transporter. The affinities of the photolabels for adenylyl cyclase were determined by their inhibition of (3H)forskolin binding to bovine brain membranes. 6-AIPP-Fsk and 7-AIPP-Fsk inhibited (3H)forskolin binding with IC50 values of 15 nM and 200 nM, respectively. 125I-6-AIPP-Fsk labeled a 115-kDa protein in control and GTP {gamma} S-preactivated bovine brain membranes. This labeling was inhibited by forskolin but not by 1,9-dideoxyforskolin or cytochalasin B. 125I-6-AIPP-Fsk labeling of partially purified adenylyl cyclase was inhibited by forskolinmore » but not by 1,9-dideoxyforskolin. 125I-7-AIPP-Fsk specifically labeled a 45-kDa protein and not a 115-kDa protein in control and GTP {gamma} S-preactivated brain membranes. This labeling was inhibited by forskolin, 1,9-dideoxyforskolin, cytochalasin B, and D-glucose but not cytochalasin E or L-glucose. Human erythrocyte membranes were photolyzed with 125I-6-AIPP-Fsk and 125I-7-AIPP-Fsk. 125I-7-AIPP-Fsk, but not 125I-6-AIPP-Fsk, strongly labeled a broad 45-70-kDa band. Forskolin, 7-bromoacetyl-7-desacetylforskolin, 1,9-dideoxyforskolin, cytochalasin B, and D-glucose, but not cytochalasin E or L-glucose, inhibited 125I-7-AIPP-Fsk labeling of the 45-70-kDa band. 125I-6-AIPP-Fsk and 125I-7-AIPP-Fsk are high affinity photolabels with specificity for adenylyl cyclase and the glucose transporter, respectively.« less

  7. Photoaffinity analogues of methotrexate as folate antagonist binding probes. 2. Transport studies, photoaffinity labeling, and identification of the membrane carrier protein for methotrexate from murine L1210 cells

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Price, E.M.; Freisheim, J.H.

    1987-07-28

    A membrane-derived component of the methotrexate/one-carbon-reduced folate transport system in murine L1210 cells has been identified by using a photoaffinity analogue of methotrexate. The compound, a radioiodinated 4-azidosalicylyl derivative of the lysine analogue of methotrexate, is transported into murine L1210 cells in a temperature-dependent, sulfhydryl reagent inhibitable manner with a K/sub t/ of 506 +/- 79 nM and a V/sub max/ of 17.9 +/- 4.2 pmol min/sup -1/ (mg of total cellular protein)/sup -1/. Uptake of the iodinated compound at 200 nM is inhibited by low amounts of methotrexate. The parent compounds of the iodinated photoprobe inhibit (/sup 3/H)methotrexate uptake,more » with the uniodinated 4-azidosalicylyl derivative exhibiting a K/sub i/ of 66 +/- 21 nM. UV irradiation, at 4 /sup 0/C, of a cell suspension that had been incubated with the probe results in the covalent modification of a 46K-48K protein. This can be demonstrated when the plasma membranes from the labeled cells are analyzed via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Labeling of this protein occurs half-maximally at a reagent concentration that correlates with the K/sub t/ for transport of the iodinated compound. Protection against labeling of this protein by increasing amounts of methotrexate parallels the concentration dependence of inhibition of photoprobe uptake by methotrexate. Evidence that, in the absence of irradiation and at 37/sup 0/C, the iodinated probe is actually internalized is demonstrated by the labeling of two soluble proteins (M/sub r/ 38K and 21K) derived from the cell homogenate supernatant.« less

  8. Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues

    PubMed Central

    Tulloch, Lindsay B.; Menzies, Stefanie K.; Fraser, Andrew L.; Gould, Eoin R.; King, Elizabeth F.; Zacharova, Marija K.; Florence, Gordon J.

    2017-01-01

    Current drugs to treat African sleeping sickness are inadequate and new therapies are urgently required. As part of a medicinal chemistry programme based upon the simplification of acetogenin-type ether scaffolds, we previously reported the promising trypanocidal activity of compound 1, a bis-tetrahydropyran 1,4-triazole (B-THP-T) inhibitor. This study aims to identify the protein target(s) of this class of compound in Trypanosoma brucei to understand its mode of action and aid further structural optimisation. We used compound 3, a diazirine- and alkyne-containing bi-functional photo-affinity probe analogue of our lead B-THP-T, compound 1, to identify potential targets of our lead compound in the procyclic form T. brucei. Bi-functional compound 3 was UV cross-linked to its target(s) in vivo and biotin affinity or Cy5.5 reporter tags were subsequently appended by Cu(II)-catalysed azide-alkyne cycloaddition. The biotinylated protein adducts were isolated with streptavidin affinity beads and subsequent LC-MSMS identified the FoF1-ATP synthase (mitochondrial complex V) as a potential target. This target identification was confirmed using various different approaches. We show that (i) compound 1 decreases cellular ATP levels (ii) by inhibiting oxidative phosphorylation (iii) at the FoF1-ATP synthase. Furthermore, the use of GFP-PTP-tagged subunits of the FoF1-ATP synthase, shows that our compounds bind specifically to both the α- and β-subunits of the ATP synthase. The FoF1-ATP synthase is a target of our simplified acetogenin-type analogues. This mitochondrial complex is essential in both procyclic and bloodstream forms of T. brucei and its identification as our target will enable further inhibitor optimisation towards future drug discovery. Furthermore, the photo-affinity labeling technique described here can be readily applied to other drugs of unknown targets to identify their modes of action and facilitate more broadly therapeutic drug design in any pathogen or

  9. Identification of the binding sites for ubiquinone and inhibitors in the Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae by photoaffinity labeling

    PubMed Central

    Ito, Takeshi; Ninokura, Satoshi; Kitazumi, Yuki; Mezic, Katherine G.; Cress, Brady F.; Koffas, Mattheos A. G.; Morgan, Joel E.; Barquera, Blanca; Miyoshi, Hideto

    2017-01-01

    The Na+-pumping NADH-quinone oxidoreductase (Na+-NQR) is the first enzyme of the respiratory chain and the main ion transporter in many marine and pathogenic bacteria, including Vibrio cholerae. The V. cholerae Na+-NQR has been extensively studied, but its binding sites for ubiquinone and inhibitors remain controversial. Here, using a photoreactive ubiquinone PUQ-3 as well as two aurachin-type inhibitors [125I]PAD-1 and [125I]PAD-2 and photoaffinity labeling experiments on the isolated enzyme, we demonstrate that the ubiquinone ring binds to the NqrA subunit in the regions Leu-32–Met-39 and Phe-131–Lys-138, encompassing the rear wall of a predicted ubiquinone-binding cavity. The quinolone ring and alkyl side chain of aurachin bound to the NqrB subunit in the regions Arg-43–Lys-54 and Trp-23–Gly-89, respectively. These results indicate that the binding sites for ubiquinone and aurachin-type inhibitors are in close proximity but do not overlap one another. Unexpectedly, although the inhibitory effects of PAD-1 and PAD-2 were almost completely abolished by certain mutations in NqrB (i.e. G140A and E144C), the binding reactivities of [125I]PAD-1 and [125I]PAD-2 to the mutated enzymes were unchanged compared with those of the wild-type enzyme. We also found that photoaffinity labeling by [125I]PAD-1 and [125I]PAD-2, rather than being competitively suppressed in the presence of other inhibitors, is enhanced under some experimental conditions. To explain these apparently paradoxical results, we propose models for the catalytic reaction of Na+-NQR and its interactions with inhibitors on the basis of the biochemical and biophysical results reported here and in previous work. PMID:28298441

  10. A novel photoaffinity ligand for the phencyclidine site of the N-methyl-D-aspartate receptor labels a Mr 120,000 polypeptide

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sonders, M.S.; Barmettler, P.; Lee, J.A.

    1990-04-25

    A radiolabeled photoaffinity ligand has been developed for the N-methyl-D-aspartate (NMDA)-preferring excitatory amino acid receptor complex. (3H)3-Azido-(5S, 10R)(+)-5-methyl-10,11-dihydro-5H- dibenzo(a,d)cyclohepten-5,10-imine (3H)3-azido-MK-801 demonstrated nearly identical affinity, density of binding sites, selectivity, pH sensitivity, and pharmacological profile in reversible binding assays with guinea pig brain homogenates to those displayed by its parent compound, MK-801. When employed in a photo-labeling protocol designed to optimize specific incorporation, (3H)3-azido-MK-801 labeled a single protein band which migrated in sodium dodecyl sulfate-polyacrylamide gels with Mr = 120,000. Incorporation of tritium into this band was completely inhibited when homogenates and (3H)3-azido-MK-801 were coincubated with 10 microM phencyclidine. These datamore » suggest that the phencyclidine site of the NMDA receptor complex is at least in part comprised of a Mr = 120,000 polypeptide.« less

  11. Shedding Light on Anesthetic Mechanisms: Application of Photoaffinity Ligands

    PubMed Central

    Woll, Kellie A.; Dailey, William P.; Brannigan, Grace; Eckenhoff, Roderic G.

    2016-01-01

    Anesthetic photoaffinity ligands have had an increasing presence within anesthesiology research. These ligands mimic parent general anesthetics, and allow investigators to study anesthetic interactions with receptors and enzymes; identify novel targets; and determine distribution within biological systems. To date nearly all general anesthetics used in medicine have a corresponding photoaffinity ligand represented in the literature. In this review we examine all aspects of the current methodologies, including ligand design, characterization and deployment. Finally we offer points of consideration and highlight the future outlook as more photoaffinity ligands emerge within the field. PMID:27464974

  12. Shedding Light on Anesthetic Mechanisms: Application of Photoaffinity Ligands.

    PubMed

    Woll, Kellie A; Dailey, William P; Brannigan, Grace; Eckenhoff, Roderic G

    2016-11-01

    Anesthetic photoaffinity ligands have had an increasing presence within anesthesiology research. These ligands mimic parent general anesthetics and allow investigators to study anesthetic interactions with receptors and enzymes; identify novel targets; and determine distribution within biological systems. To date, nearly all general anesthetics used in medicine have a corresponding photoaffinity ligand represented in the literature. In this review, we examine all aspects of the current methodologies, including ligand design, characterization, and deployment. Finally we offer points of consideration and highlight the future outlook as more photoaffinity ligands emerge within the field.

  13. Allosteric nature of P2X receptor activation probed by photoaffinity labelling

    PubMed Central

    Bhargava, Y; Rettinger, J; Mourot, A

    2012-01-01

    BACKGROUND AND PURPOSE In P2X receptors, agonist binding at the interface between neighbouring subunits is efficiently transduced to ion channel gating. However, the relationship between binding and gating is difficult to study because agonists continuously bind and unbind. Here, we covalently incorporated agonists in the binding pocket of P2X receptors and examined how binding site occupancy affects the ability of the channel to gate. EXPERIMENTAL APPROACH We used a strategy for tethering agonists to their ATP-binding pocket, while simultaneously probing ion channel gating using electrophysiology. The agonist 2′,3′-O-(4-benzoylbenzoyl)-ATP (BzATP), a photoaffinity analogue of ATP, enabled us to trap rat homomeric P2X2 receptor and a P2X2/1 receptor chimera in different agonist-bound states. UV light was used to control the degree of covalent occupancy of the receptors. KEY RESULTS Irradiation of the P2X2/1 receptor chimera – BzATP complex resulted in a persistent current that lasted even after extensive washout, consistent with photochemical tethering of the agonist BzATP and trapping of the receptors in an open state. Partial labelling with BzATP primed subsequent agonist binding and modulated gating efficiency for both full and partial agonists. CONCLUSIONS AND IMPLICATIONS Our photolabelling strategy provides new molecular insights into the activation mechanism of the P2X receptor. We show here that priming with full agonist molecules leads to an increase in gating efficiency after subsequent agonist binding. PMID:22725669

  14. Unambiguous Identification of β-Tubulin as the Direct Cellular Target Responsible for the Cytotoxicity of Chalcone by Photoaffinity Labeling.

    PubMed

    Zhou, Bo; Yu, Xingxin; Zhuang, Chunlin; Villalta, Peter; Lin, Yong; Lu, Junxuan; Xing, Chengguo

    2016-07-05

    Chalcone is a simple and potentially privileged structure in medicinal chemistry with a diverse repertoire of biological activities, among which cytotoxicity is of particular interest. The sharp structure-activity relationship (SAR) for chalcone's cytotoxicity suggests structure-specific target interactions. Despite the numerous putative targets proposed, evidence for direct target interactions in cells is unavailable. In this study, guided by the sharp cytotoxic SAR, we developed a cytotoxic chalcone-based photoaffinity labeling (PAL) probe, (E)-3-(3-azidophenyl)-1-[3,5-dimethoxy-4-(prop-2-yn-1-yloxy)phenyl]-2-methylprop-2-en-1-one (C95; IC50 : 0.38±0.01 μm), along with two structurally similar non-cytotoxic probes. These probes were used to search for the direct cellular target responsible for chalcone's cytotoxicity through intact cell-based PAL experiments, in which β-tubulin was identified to specifically interact with the cytotoxic probe (i.e., C95) but not the non-cytotoxic probes. A set of phenotypical and biochemical assays further reinforced β-tubulin as the cytotoxic target of chalcones. Peptide mass quantitation by mass spectrometric analysis revealed one peptide potentially labeled by C95, providing information on chalcone's binding site on β-tubulin. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Photoaffinity labeling the substance P receptor using a derivative of substance P containing para-benzoylphenylalanine

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Boyd, N.D.; White, C.F.; Leeman, S.E.

    A novel photoreactive substance P (SP) analogue has been synthesized by solid-phase peptide synthesis methodology to incorporate the amino acid p-benzoyl-L-phenylalanine (L-Phe(pBz)) in place of the Phe{sup 8} residue of SP. (Phe{sup 8}(OpBz))SP was equipotent with SP in competing for SP binding sites on rat submaxillary gland membranes and had potent sialagogic activity in vivo. In the absence of light, the {sup 125}I-labeled Bolton-Hunter conjugate of (Phe{sup 8}(pBz))SP bound in a saturable and reversible manner to an apparently homogeneous class of binding sites with an affinity K{sub D} = 0.4 nM. The binding of {sup 125}I-(Phe{sup 8}(pBz))SP was inhibited competitivelymore » by various tachykinin peptides and analogues with the appropriate specificity for SP/NK-1 receptors. Upon photolysis, up to 70{percent} of the specifically bound {sup 125}I-(Phe{sup 8}(pBz))SP underwent covalent linkage to two polypeptides of M{sub r} = 53 000 and 46 000, identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Quantitative analysis of the inhibitory effects of SP and related peptides on {sup 125}I-(Phe{sup 8}(pBz))SP photoincorporation indicated that the binding sites of the two photolabeled polypeptides have the same peptide specificity, namely, that typical of NK-1-type SP receptors. Further information on the relationship between the two labeled SP binding sites was provided by enzymatic digestion studies. The highly specific and remarkably efficient photolabeling achieved with {sup 125}I-(Phe{sup 8}(pBz))SP suggests that this photoaffinity probe will be of considerable value for the characterization of the molecular structure of the SP receptor.« less

  16. Adrenocortical nuclear progesterone-binding protein: Identification by photoaffinity labeling and evidence for deoxyribonucleic acid binding and stimulation by adrenocorticotropin

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Demura, T.; Driscoll, W.J.; Lee, Y.C.

    1991-01-01

    Nuclei of the guinea pig adrenal cortex contain a protein that specifically binds progesterone and that, biochemically, is clearly distinct from the classical progesterone receptor. The adrenocortical nuclear progesterone-binding protein has now been purified more than 2000-fold by steroid-affinity chromatography with a 75% yield. The purified protein preparation demonstrated three major bands on sodium dodecyl sulfate-polyacrylamide gel of 79K, 74K, and 50K. To determine which of the three might represent the progesterone-binding protein, steroid photoaffinity labeling was performed which resulted in the specific and exclusive labeling of a 50K band. Thus, the adrenocortical nuclear progesterone-binding protein appears to be distinctmore » from the classical progesterone receptor not only biochemically, but also on the basis of molecular size. To test whether the adrenocortical nuclear progesterone-binding protein can be hormonally stimulated, guinea pigs were treated with ACTH. The chronic administration of ACTH caused a 4- to 6-fold increase in the specific progesterone binding capacity without a change in the binding affinity. There appeared to be no significant difference in nuclear progesterone binding between the zona fasciculata and zona reticularis. This finding suggests a mediating role for the progesterone-binding protein in ACTH action. In addition, the nuclear progesterone-binding protein bound to nonspecific DNA sequences, further suggesting a possible transcriptional regulatory role.« less

  17. A fluorescent radioiodinated oligonucleotidic photoaffinity probe for protein labeling: synthesis and photolabeling of thrombin.

    PubMed

    Berens, C; Courtoy, P J; Sonveaux, E

    1999-01-01

    To study the interactions between oligonucleotides and proteins, an original photoaffinity radiolabeling probe has been synthesized. Starting with a 5'-pyridyldithio-3'-amino-oligonucleotide, the photophore benzophenone was first coupled to the 3' end, through acylation by an activated ester of benzoylbenzoic acid. A fluorescein molecule was grafted by alkylation of the free 5'-SH. This compound was finally radiolabeled with 125I using IodoBeads. The selective photolabeling of thrombin in a complex protein mixture by the radioiodinated probe validates this strategy to identify oligonucleotide-binding proteins.

  18. Optimizing a three-stage Compton camera for measuring prompt gamma rays emitted during proton radiotherapy

    PubMed Central

    Peterson, S W; Robertson, D; Polf, J

    2011-01-01

    In this work, we investigate the use of a three-stage Compton camera to measure secondary prompt gamma rays emitted from patients treated with proton beam radiotherapy. The purpose of this study was (1) to develop an optimal three-stage Compton camera specifically designed to measure prompt gamma rays emitted from tissue and (2) to determine the feasibility of using this optimized Compton camera design to measure and image prompt gamma rays emitted during proton beam irradiation. The three-stage Compton camera was modeled in Geant4 as three high-purity germanium detector stages arranged in parallel-plane geometry. Initially, an isotropic gamma source ranging from 0 to 15 MeV was used to determine lateral width and thickness of the detector stages that provided the optimal detection efficiency. Then, the gamma source was replaced by a proton beam irradiating a tissue phantom to calculate the overall efficiency of the optimized camera for detecting emitted prompt gammas. The overall calculated efficiencies varied from ~10−6 to 10−3 prompt gammas detected per proton incident on the tissue phantom for several variations of the optimal camera design studied. Based on the overall efficiency results, we believe it feasible that a three-stage Compton camera could detect a sufficient number of prompt gammas to allow measurement and imaging of prompt gamma emission during proton radiotherapy. PMID:21048295

  19. Enterohepatic circulation in man of a gamma-emitting bile-acid conjugate, 23-selena-25-homotaurocholic acid (SeHCAT).

    PubMed

    Merrick, M V; Eastwood, M A; Anderson, J R; Ross, H M

    1982-02-01

    A conjugated bile acid, 23-selena-25-homotaurocholic acid (SeHCAT), labeled with the gamma emitter Se-75, has been evaluated in man. Absorption and excretion were compared with that of simultaneously administered [23-14C]cholic acid. SeHCAT is absorbed quantitatively following oral administration, secreted into the bile at the same rate as cholic acid, reabsorbed from the small intestine, and resecreted. It is not absorbed when the terminal ileum has been excised or bypassed. SeHCAT is therefore the first of a new class of radiopharmaceuticals, namely, gamma-emitting tracers of the complete cycle of the enterohepatic circulation. Its use will simplify investigation of the functional state of the terminal ileum by eliminating the need to collect and process feces.

  20. Photoaffinity labeling of the TF1-ATPase from the thermophilic bacterium PS3 with 3'-O-(4-benzoyl)benzoyl ADP.

    PubMed

    Bar-Zvi, D; Yoshida, M; Shavit, N

    1985-05-31

    3'-O-(4-Benzoyl)benzoyl ADP (BzADP) was used as a photoaffinity label for covalent binding of adenine nucleotide analogs to the nucleotide binding site(s) of the thermophilic bacterium PS3 ATPase (TF1). As with the CF1-ATPase (Bar-Zvi, D. and Shavit, N. (1984) Biochim. Biophys. Acta 765, 340-356) noncovalently bound BzADP is a reversible inhibitor of the TF1-ATPase. BzADP changes the kinetics of ATP hydrolysis from noncooperative to cooperative in the same way as ADP does, but, in contrast to the effect on the CF1-ATPase, it has no effect on the Vmax. In the absence of Mg2+ 1 mol BzADP binds noncovalently to TF1, while with Mg2+ 3 mol are bound. Photoactivation of BzADP results in the covalent binding of the analog to the nucleotide binding site(s) on TF1 and correlates with the inactivation of the ATPase. Complete inactivation of the TF1-ATPase occurs after covalent binding of 2 mol BzADP/mol TF1. Photoinactivation of TF1 by BzADP is prevented if excess of either ADP or ATP is present during irradiation. Analysis by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate of the Bz[3H]ADP-labeled TF1-ATPase shows that all the radioactivity is incorporated into the beta subunit.

  1. Evidence of the photosynthetic origin of monoterpenes emitted by quercus ilex L. leaves by {sup 13}C labeling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Loreto, F.; Ciccioli, P.; Cecinato, A.

    1996-04-01

    The carbon of the four main monoterpenes emitted by Quercus ilex L. leaves was completely labeled with {sup 13}C after a 20-min feeding with 99% {sup 13}CO{sub 2}. This labeling time course is comparable with the labeling time course of isoprene, the terpenoid emitted by other Quercus species and synthesized in leaf chloroplasts. It is also comparable with that of phosphoglyceric acid. Our experiment therefore provides evidence that monoterpenes emitted by Q. ilex are formed photosynthesis intermediates and may share the same synthetic pathway with isoprene. By analyzing the rate and the distribution of labeling in the different fragments, wemore » looked for evidence of differential carbon labeling in the {alpha}-pinene emitted. However, the labeling pattern was quite uniform in the different fragments, suggesting that the carbon skeleton of the emitted monoterpenes comes from a unique carbon source. 16 refs., 3 figs., 1 tab.« less

  2. Sex pheromone receptor proteins. Visualization using a radiolabeled photoaffinity analog

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Vogt, R.G.; Prestwich, G.D.; Riddiford, L.M.

    1988-03-15

    A tritium-labeled photoaffinity analog of a moth pheromone was used to covalently modify pheromone-selective binding proteins in the antennal sensillum lymph and sensory dendritic membranes of the male silk moth, Antheraea polyphemus. This analog, (E,Z)-6,11-(/sup 3/H)hexadecadienyl diazoacetate, allowed visualization of a 15-kilodalton soluble protein and a 69-kilodalton membrane protein in fluorescence autoradiograms of electrophoretically separated antennal proteins. Covalent modification of these proteins was specifically reduced when incubation and UV irradiation were conducted in the presence of excess unlabeled pheromone, (E,Z)-6,11-hexadecadienyl acetate. These experiments constitute the first direct evidence for a membrane protein of a chemosensory neuron interacting in a specificmore » fashion with a biologically relevant odorant.« less

  3. Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs.

    PubMed

    Gao, Jinxu; Mfuh, Adelphe; Amako, Yuka; Woo, Christina M

    2018-03-28

    Many therapeutics elicit cell-type specific polypharmacology that is executed by a network of molecular recognition events between a small molecule and the whole proteome. However, measurement of the structures that underpin the molecular associations between the proteome and even common therapeutics, such as the nonsteroidal anti-inflammatory drugs (NSAIDs), is limited by the inability to map the small molecule interactome. To address this gap, we developed a platform termed small molecule interactome mapping by photoaffinity labeling (SIM-PAL) and applied it to the in cellulo direct characterization of specific NSAID binding sites. SIM-PAL uses (1) photochemical conjugation of NSAID derivatives in the whole proteome and (2) enrichment and isotope-recoding of the conjugated peptides for (3) targeted mass spectrometry-based assignment. Using SIM-PAL, we identified the NSAID interactome consisting of over 1000 significantly enriched proteins and directly characterized nearly 200 conjugated peptides representing direct binding sites of the photo-NSAIDs with proteins from Jurkat and K562 cells. The enriched proteins were often identified as parts of complexes, including known targets of NSAID activity (e.g., NF-κB) and novel interactions (e.g., AP-2, proteasome). The conjugated peptides revealed direct NSAID binding sites from the cell surface to the nucleus and a specific binding site hotspot for the three photo-NSAIDs on histones H2A and H2B. NSAID binding stabilized COX-2 and histone H2A by cellular thermal shift assay. Since small molecule stabilization of protein complexes is a gain of function regulatory mechanism, it is conceivable that NSAIDs affect biological processes through these broader proteomic interactions. SIM-PAL enabled characterization of NSAID binding site hotspots and is amenable to map global binding sites for virtually any molecule of interest.

  4. Immunophotoaffinity labeling of binders of 1-methyladenine, the oocyte maturation-inducing hormone of starfish

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Toraya, Tetsuo; Kida, Tetsuo; Kuyama, Atsushi

    Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary and resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the oocyte maturation-inducing hormone of starfish. Putative 1-MeAde receptors on the oocyte surface have been suggested, but not yet been biochemically characterized. Immunophotoaffinity labeling, i.e., photoaffinity labeling combined with immunochemical detection, was attempted to detect unknown 1-MeAde binders including putative maturation-inducing hormone receptors in starfish oocytes. When the oocyte crude membrane fraction or its Triton X-100/EDTA extract was incubated with N{sup 6}-[6-(5-azido-2-nitrobenzoyl)aminohexyl]carboxamidomethyl-1-methyladenine and then photo-irradiated, followed by western blotting with antibody that was raised againstmore » a 1-MeAde hapten, a single band with M{sub r} of 47.5 K was detected. The band was lost when extract was heated at 100 °C. A similar 47.5 K band was detected in the crude membrane fraction of testis as well. Upon labeling with whole cells, this band was detected in immature and maturing oocytes, but only faintly in mature oocytes. As judged from these results, this 1-MeAde binder might be a possible candidate of the starfish maturation-inducing hormone receptors. - Highlights: • Synthesis of photoaffinity labeling reagents for 1-methyladenine binders of starfish. • Immunochemical detection of photoaffinity-labeled 1-methyladenine binders. • Immunophotoaffinity labeling of a 47.5 K 1-methyladenine binder in oocytes and testis. • A possible candidate of oocyte maturation-inducing hormone receptors of starfish.« less

  5. Evaluation of radiation dose to anthropomorphic paediatric models from positron-emitting labelled tracers

    NASA Astrophysics Data System (ADS)

    Xie, Tianwu; Zaidi, Habib

    2014-03-01

    PET uses specific molecules labelled with positron-emitting radionuclides to provide valuable biochemical and physiological information. However, the administration of radiotracers to patients exposes them to low-dose ionizing radiation, which is a concern in the paediatric population since children are at a higher cancer risk from radiation exposure than adults. Therefore, radiation dosimety calculations for commonly used positron-emitting radiotracers in the paediatric population are highly desired. We evaluate the absorbed dose and effective dose for 19 positron-emitting labelled radiotracers in anthropomorphic paediatric models including the newborn, 1-, 5-, 10- and 15-year-old male and female. This is achieved using pre-calculated S-values of positron-emitting radionuclides of UF-NCI paediatric phantoms and published biokinetic data for various radiotracers. The influence of the type of anthropomorphic model, tissue weight factors and direct human- versus mouse-derived biokinetic data on the effective dose for paediatric phantoms was also evaluated. In the case of 18F-FDG, dosimetry calculations of reference paediatric patients from various dose regimens were also calculated. Among the considered radiotracers, 18F-FBPA and 15O-water resulted in the highest and lowest effective dose in the paediatric phantoms, respectively. The ICRP 103 updated tissue-weighting factors decrease the effective dose in most cases. Substantial differences of radiation dose were observed between direct human- versus mouse-derived biokinetic data. Moreover, the effect of using voxel- versus MIRD-type models on the calculation of the effective dose was also studied. The generated database of absorbed organ dose and effective dose for various positron-emitting labelled radiotracers using new generation computational models and the new ICRP tissue-weighting factors can be used for the assessment of radiation risks to paediatric patients in clinical practice. This work also contributes

  6. Ecdysteroid 7,9(11)-dien-6-ones as potential photoaffinity labels for ecdysteroid binding proteins

    PubMed Central

    Bourne, Pauline C.; Whiting, Pensri; Dhadialla, Tarlochan S.; Hormann, Robert E.; Girault, Jean-Pierre; Harmatha, Juraj; Lafont, René; Dinan, Laurence

    2002-01-01

    Three ecdysteroid 7,9(11)-dien-7-ones (dacryhainansterone, 25-hydroxydacryhainansterone and kaladasterone) were prepared by dehydration of the corresponding 11a-hydroxy ecdysteroids (ajugasterone C, turkesterone and muristerone A, respectively). The biological activities of the dienones in the Drosophila melanogaster BII cell bioassay, which reflect the affinity for the ecdysteroid receptor complex, showed that the dienones retain high biological activity. Irradiation at 350 nm of the ecdysteroid dienones (100 nM) with bacterially-expressed dipteran and lepidopteran ecdysteroid receptor proteins (DmEcR/DmUSP or CfEcR/CfUSP), followed by loading with [3H]ponasterone A revealed that irradiation of dacryhainansterone or kaladasterone resulted in blocking of >70% of the specific binding sites. Thus, ecdysteroid dienones show considerable potential as photoaffinity analogues for ecdysteroid binding proteins. PMID:15455045

  7. Contributions of Torpedo nicotinic acetylcholine receptor gamma Trp-55 and delta Trp-57 to agonist and competitive antagonist function.

    PubMed

    Xie, Y; Cohen, J B

    2001-01-26

    Results of affinity-labeling studies and mutational analyses provide evidence that the agonist binding sites of the nicotinic acetylcholine receptor (nAChR) are located at the alpha-gamma and alpha-delta subunit interfaces. For Torpedo nAChR, photoaffinity-labeling studies with the competitive antagonist d-[(3)H]tubocurarine (dTC) identified two tryptophans, gammaTrp-55 and deltaTrp-57, as the primary sites of photolabeling in the non-alpha subunits. To characterize the importance of gammaTrp-55 and deltaTrp-57 to the interactions of agonists and antagonists, Torpedo nAChRs were expressed in Xenopus oocytes, and equilibrium binding assays and electrophysiological recordings were used to examine the functional consequences when either or both tryptophans were mutated to leucine. Neither substitution altered the equilibrium binding of dTC. However, the deltaW57L and gammaW55L mutations decreased acetylcholine (ACh) binding affinity by 20- and 7,000-fold respectively. For the wild-type, gammaW55L, and deltaW57L nAChRs, the concentration dependence of channel activation was characterized by Hill coefficients of 1.8, 1.1, and 1.7. For the gammaW55L mutant, dTC binding at the alpha-gamma site acts not as a competitive antagonist but as a coactivator or partial agonist. These results establish that interactions with gamma Trp-55 of the Torpedo nAChR play a crucial role in agonist binding and in the agonist-induced conformational changes that lead to channel opening.

  8. Identification of amino acids in the nicotinic acetylcholine receptor agonist binding site and ion channel photolabeled by 4-[(3-trifluoromethyl)-3H-diazirin-3-yl]benzoylcholine, a novel photoaffinity antagonist.

    PubMed

    Chiara, David C; Trinidad, Jonathan C; Wang, Dong; Ziebell, Michael R; Sullivan, Deirdre; Cohen, Jonathan B

    2003-01-21

    [(3)H]4-[(3-trifluoromethyl)-3H-diazirin-3-yl]benzoylcholine (TDBzcholine) was synthesized and used as a photoaffinity probe to map the orientation of an aromatic choline ester within the agonist binding sites of the Torpedo nicotinic acetylcholine receptor (nAChR). TDBzcholine acts as a nAChR competitive antagonist that binds at equilibrium with equal affinity to both agonist sites (K(D) approximately 10 microM). Upon UV irradiation (350 nm), nAChR-rich membranes equilibrated with [(3)H]TDBzcholine incorporate (3)H into the alpha, gamma, and delta subunits in an agonist-inhibitable manner. The specific residues labeled by [(3)H]TDBzcholine were determined by N-terminal sequence analysis of subunit fragments produced by enzymatic cleavage and purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and/or reversed-phase high-performance liquid chromatography. For the alpha subunit, [(3)H]TDBzcholine photoincorporated into alphaCys-192, alphaCys-193, and alphaPro-194. For the gamma and delta subunits, [(3)H]TDBzcholine incorporated into homologous leucine residues, gammaLeu-109 and deltaLeu-111. The photolabeling of these amino acids suggests that when the antagonist TDBzcholine occupies the agonist binding sites, the Cys-192-193 disulfide and Pro-194 from the alpha subunit Segment C are oriented toward the agonist site and are in proximity to gammaLeu-109/deltaLeu-111 in Segment E, a conclusion consistent with the structure of the binding site in the molluscan acetylcholine binding protein, a soluble protein that is homologous to the nAChR extracellular domain.

  9. Photoaffinity Labeling via Nitrenium Ion Chemistry: Protonation of the Nitrene Derived from a 4-Amino-3-nitrophenylazide to Afford Reactive Nitrenium Ion Pairs

    PubMed Central

    Voskresenska, Valentyna; Wilson, R. Marshall; Panov, Maxim; Tarnovsky, Alexander N.; Krause, Jeanette A.; Vyas, Shubham; Winter, Arthur H.; Hadad, Christopher M.

    2009-01-01

    Phenyl azides with powerful electron-donating substituents are known to deviate from the usual photochemical behavior of other phenyl azides. They do not undergo ring expansion, but form basic nitrenes that protonate to form nitrenium ions. The photochemistry of the widely used photoaffinity labeling system 4-amino-3-nitrophenyl azide, 5, has been studied by transient absorption spectroscopy from femtosecond to microsecond time domains and from a theoretical perspective. The nitrene generation from azide 5 occurs on the S2 surface, in violation of Kasha's rule. The resulting nitrene is a powerful base and abstracts protons extremely rapidly from a variety of sources to form a nitrenium ion. In methanol, this protonation occurs in about 5 ps, which is the fastest intermolecular protonation observed to date. Suitable proton sources include alcohols, amine salts, and even acidic C-H bonds such as acetonitrile. The resulting nitrenium ion is stabilized by the electron-donating 4-amino group to afford a diiminoquinone-like species that collapses relatively slowly to form the ultimate cross-linked product. In some cases in which the anion is a good hydride donor, cross-linking is replaced by reduction of the nitrenium ion to the corresponding amine. PMID:19624129

  10. Identification of the uridine 5'-diphosphoglucose (UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido-UDP-Glc

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lin, F.C.; Brown, R.M. Jr.; Drake, R.R. Jr.

    1990-03-25

    Photoaffinity labeling of purified cellulose synthase with (beta-32P)5-azidouridine 5'-diphosphoglucose (UDP-Glc) has been used to identify the UDP-Glc binding subunit of the cellulose synthase from Acetobacter xylinum strain ATCC 53582. The results showed exclusive labeling of an 83-kDa polypeptide. Photoinsertion of (beta-32P)5-azido-UDP-Glc is stimulated by the cellulose synthase activator, bis-(3'----5') cyclic diguanylic acid. Addition of increasing amounts of UDP-Glc prevents photolabeling of the 83-kDa polypeptide. The reversible and photocatalyzed binding of this photoprobe also showed saturation kinetics. These studies demonstrate that the 83-kDa polypeptide is the catalytic subunit of the cellulose synthase in A. xylinum strain ATCC 53582.

  11. Identification of endoplasmic reticulum proteins involved in glycan assembly: synthesis and characterization of P3-(4-azidoanilido)uridine 5'-triphosphate, a membrane-topological photoaffinity probe for uridine diphosphate-sugar binding proteins.

    PubMed Central

    Rancour, D M; Menon, A K

    1998-01-01

    Much of the enzymic machinery required for the assembly of cell surface carbohydrates is located in the endoplasmic reticulum (ER) of eukaryotic cells. Structural information on these proteins is limited and the identity of the active polypeptide(s) is generally unknown. This paper describes the synthesis and characteristics of a photoaffinity reagent that can be used to identify and analyse members of the ER glycan assembly apparatus, specifically those glycosyltransferases, nucleotide phosphatases and nucleotide-sugar transporters that recognize uridine nucleotides or UDP-sugars. The photoaffinity reagent, P3-(4-azidoanilido)uridine 5'-triphosphate (AAUTP), was synthesized easily from commercially available precursors. AAUTP inhibited the activity of ER glycosyltransferases that utilize UDP-GlcNAc and UDP-Glc, indicating that it is recognized by UDP-sugar-binding proteins. In preliminary tests AAUTP[alpha-32P] labelled bovine milk galactosyltransferase, a model UDP-sugar-utilizing enzyme, in a UV-light-dependent, competitive and saturable manner. When incubated with rat liver ER vesicles, AAUTP[alpha-32P] labelled a discrete subset of ER proteins; labelling was light-dependent and metal ion-specific. Photolabelling of intact ER vesicles with AAUTP[alpha-32P] caused selective incorporation of radioactivity into proteins with cytoplasmically disposed binding sites; UDP-Glc:glycoprotein glucosyltransferase, a lumenal protein, was labelled only when the vesicle membrane was disrupted. These data indicate that AAUTP is a membrane topological probe of catalytic sites in target proteins. Strategies for using AAUTP to identify and study novel ER proteins involved in glycan assembly are discussed. PMID:9677326

  12. Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure.

    PubMed

    Kramer, W; Sauber, K; Baringhaus, K H; Kurz, M; Stengelin, S; Lange, G; Corsiero, D; Girbig, F; König, W; Weyland, C

    2001-03-09

    The ileal lipid-binding protein (ILBP) is the only physiologically relevant bile acid-binding protein in the cytosol of ileocytes. To identify the bile acid-binding site(s) of ILBP, recombinant rabbit ILBP photolabeled with 3-azi- and 7-azi-derivatives of cholyltaurine was analyzed by a combination of enzymatic fragmentation, gel electrophoresis, and matrix-assisted laser desorption ionization (MALDI)-mass spectrometry. The attachment site of the 3-position of cholyltaurine was localized to the amino acid triplet His(100)-Thr(101)-Ser(102) using the photoreactive 3,3-azo-derivative of cholyltaurine. With the corresponding 7,7-azo-derivative, the attachment point of the 7-position could be localized to the C-terminal part (position 112-128) as well as to the N-terminal part suggesting more than one binding site for bile acids. By chemical modification and NMR structure of ILBP, arginine residue 122 was identified as the probable contact point for the negatively charged side chain of cholyltaurine. Consequently, bile acids bind to ILBP with the steroid nucleus deep inside the protein cavity and the negatively charged side chain near the entry portal. The combination of photoaffinity labeling, enzymatic fragmentation, MALDI-mass spectrometry, and NMR structure was successfully used to determine the topology of bile acid binding to ILBP.

  13. Photoaffinity labeling of the human erythrocyte glucose transporter with /sup 4/H-labelled forskolin

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shanahan, M.F.; Edwards, B.M.; Morris, D.P.

    1986-05-01

    Forskolin, a potent activator of adenylate cyclase, is also known to inhibit glucose transport in a number of cells. The authors have investigated photoincorporation of (/sup 3/H)forskolin into erythrocyte membrane proteins using a technique they previously developed for photolabeling the erythrocyte glucose transporter with cytochalasin B (CB). A 30-40s irradiation of erythrocyte ghosts in the presence of (/sup 3/H)forskolin resulted in a concentration-dependent, covalent incorporation of radiolabel into all of the major membrane protein bands. However, most of the incorporation occurred in only three regions of the gel. Peak 1 was a sharp peak near the top of the gelmore » in the region corresponding to spectrin, peak 2 appeared to be associated with band 3 (approx. 90kDa), and the third region labeled was between 41-60 kDa which corresponds to the region of the glucose transporter. This region appeared to contain several overlapping peaks with the largest incorporation of label occurring around 45 kDa in the area of red cell actin. When photolabeling was performed in the presence of 400 ..mu..M cytochalasin B (8.0 ..mu..M forskolin) the labeling in the 41-60 kDa region was totally inhibited while labeling of the 90 kDa peak was partially blocked. CB had no effect on the photolabeling of peak 1 by forskolin.« less

  14. METHOD FOR THE STUDY OF THE LIVER BLOOD FLOW USING GAMMA-EMITTING RADIONUCLIDES

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Baptista, A.M.; Carvalho, J.S.

    1959-10-31

    A method is described for the evaluation of liver blood flow values by radioactivity measurements of gamma-emitting radionuclides, in colloid form, using a scintillation detector positioned over the liver region. It is shown that the disappearance rate constant of the nuclide from the blood can be calculated from the curves obtained. Advantages of the method, including the use of small amounts of radioactive materials, are discussed. (auth)

  15. Recombining plasma in the gamma-ray-emitting mixed-morphology supernova remnant 3C 391

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ergin, T.; Sezer, A.; Saha, L.

    2014-07-20

    A group of middle-aged mixed-morphology (MM) supernova remnants (SNRs) interacting with molecular clouds (MCs) has been discovered to be strong GeV gamma-ray emitters by the Large Area Telescope (LAT) on board the Fermi Gamma-Ray Space Telescope (Fermi-LAT). The recent observations of the Suzaku X-ray satellite have revealed that some of these interacting gamma-ray-emitting SNRs, such as IC443, W49B, W44, and G359.1-0.5, have overionized plasmas. 3C 391 (G31.9+0.0) is another Galactic MM SNR interacting with MCs. It was observed in GeV gamma rays by Fermi-LAT as well as in the 0.3-10.0 keV X-ray band by Suzaku. In this work, 3C 391more » was detected in GeV gamma rays with a significance of ∼18σ and we showed that the GeV emission is point-like in nature. The GeV gamma-ray spectrum was shown to be best explained by the decay of neutral pions assuming that the protons follow a broken power-law distribution. We revealed radiative recombination structures of silicon and sulfur from 3C 391 using Suzaku data. In this paper, we discuss the possible origin of this type of radiative plasma and hadronic gamma rays.« less

  16. Phosphatidylinositol 3,4,5-trisphosphate activity probes for the labeling and proteomic characterization of protein binding partners.

    PubMed

    Rowland, Meng M; Bostic, Heidi E; Gong, Denghuang; Speers, Anna E; Lucas, Nathan; Cho, Wonhwa; Cravatt, Benjamin F; Best, Michael D

    2011-12-27

    Phosphatidylinositol polyphosphate lipids, such as phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P₃], regulate critical biological processes, many of which are aberrant in disease. These lipids often act as site-specific ligands in interactions that enforce membrane association of protein binding partners. Herein, we describe the development of bifunctional activity probes corresponding to the headgroup of PI(3,4,5)P₃ that are effective for identifying and characterizing protein binding partners from complex samples, namely cancer cell extracts. These probes contain both a photoaffinity tag for covalent labeling of target proteins and a secondary handle for subsequent detection or manipulation of labeled proteins. Probes bearing different secondary tags were exploited, either by direct attachment of a fluorescent dye for optical detection or by using an alkyne that can be derivatized after protein labeling via click chemistry. First, we describe the design and modular synthetic strategy used to generate multiple probes with different reporter tags of use for characterizing probe-labeled proteins. Next, we report initial labeling studies using purified protein, the PH domain of Akt, in which probes were found to label this target, as judged by in-gel detection. Furthermore, protein labeling was abrogated by controls including competition with an unlabeled PI(3,4,5)P₃ headgroup analogue as well as through protein denaturation, indicating specific labeling. In addition, probes featuring linkers of different lengths between the PI(3,4,5)P₃ headgroup and photoaffinity tag led to variations in protein labeling, indicating that a shorter linker was more effective in this case. Finally, proteomic labeling studies were performed using cell extracts; labeled proteins were observed by in-gel detection and characterized using postlabeling with biotin, affinity chromatography, and identification via tandem mass spectrometry. These studies yielded a total of 265

  17. SEARCHING FOR OVERIONIZED PLASMA IN THE GAMMA-RAY-EMITTING SUPERNOVA REMNANT G349.7+0.2

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ergin, T.; Sezer, A.; Saha, L.

    2015-05-10

    G349.7+0.2 is a supernova remnant (SNR) expanding in a dense medium of molecular clouds and interacting with clumps of molecular material emitting gamma-rays. We analyzed the gamma-ray data of the Large Area Telescope on board the Fermi Gamma-Ray Space Telescope and detected G349.7+0.2 in the energy range of 0.2–300 GeV with a significance of ∼13σ, showing no extended morphology. Modeling of the gamma-ray spectrum revealed that the GeV gamma-ray emission dominantly originates from the decay of neutral pions, where the protons follow a broken power-law distribution with a spectral break at ∼12 GeV. To search for features of radiative recombinationmore » continua in the eastern and western regions of the remnant, we analyzed the Suzaku data of G349.7+0.2 and found no evidence for overionized plasma. In this paper, we discuss possible scenarios to explain the hadronic gamma-ray emission in G349.7+0.2 and the mixed morphology nature of this SNR.« less

  18. Synthesis, characterization and inhibitory activities of (4-N3[3,5-3H]Phe10)PKI(6-22)amide and its precursors: photoaffinity labeling peptides for the active site of cyclic AMP-dependent protein kinase.

    PubMed

    Katz, B M; Lundquist, L J; Walsh, D A; Glass, D B

    1989-06-01

    PKI(6-22)amide is a 17 residue peptide corresponding to the active portion of the heat-stable inhibitor of cAMP-dependent protein kinase. The peptide is a potent (Ki = 1.6 nM), competitive inhibitor of the enzyme. The photoreactive peptide analog (4-azidophenylalanine10)PKI(6-22)amide was synthesized in both its non-radiolabeled and tritiated forms by chemical modification of precursor peptides that were prepared by stepwise solid-phase synthesis. (4-Amino[3,5-3H]phenylalanine10)PKI(6-22)amide, the precursor for the radiolabeled arylazide peptide, was obtained by catalytic reduction of the corresponding peptide containing the 3,5-diiodo-4-aminophenylalanine residue at position 10. The purified PKI peptides were analyzed by HPLC, amino acid analysis, and u.v. spectra. In the dark, (4-azidophenylalanine10)PKI(6-22)amide inhibited the catalytic subunit of cAMP-dependent protein kinase with a Ki value of 2.8 nM. The photoreactivity of the arylazide peptide was demonstrated by time-dependent u.v. spectral changes on exposure to light. Photolysis of the catalytic subunit (4-azido[3,5-3H]phenylalanine10)PKI(6-22)amide complex resulted in specific covalent labeling of the enzyme. The data indicate that this peptide is a useful photoaffinity labeling reagent for the active site of the protein kinase.

  19. Apparatus for reading two-dimensional electrophoretograms containing. beta. -ray-emitting labeled compounds

    DOEpatents

    Anderson, H.L.; Kinnison, W.W.; Lillberg, J.W.

    1985-04-30

    An apparatus and method for electronically reading planar two-dimensional ..beta..-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the ..beta..-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700-..mu..m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained in less than one hour.

  20. Phosphatidylinositol (3,4,5)-Trisphosphate Activity Probes for the Labeling and Proteomic Characterization of Protein Binding Partners

    PubMed Central

    Rowland, Meng M.; Bostic, Heidi E.; Gong, Denghuang; Speers, Anna E.; Lucas, Nathan; Cho, Wonhwa; Cravatt, Benjamin F.; Best, Michael D.

    2013-01-01

    Phosphatidylinositol polyphosphate lipids, such as phosphatidylinositol (3,4,5)-trisphosphate (PI(3,4,5)P3), regulate critical biological processes, many of which are aberrant in disease. These lipids often act as site-specific ligands in interactions that enforce membrane-association of protein binding partners. Herein, we describe the development of bifunctional activity probes corresponding to the headgroup of PI(3,4,5)P3 that are effective for identifying and characterizing protein binding partners from complex samples, namely cancer cell extracts. These probes contain both a photoaffinity tag for covalent labeling of target proteins as well as a secondary handle for subsequent detection or manipulation of labeled proteins. Probes bearing different secondary tags were exploited, either by direct attachment of a fluorescent dye for optical detection or by using an alkyne that can be derivatized after protein labeling via click chemistry. First, we describe the design and modular synthetic strategy used to generate multiple probes with different reporter tags of use for characterizing probe-labeled proteins. Next, we report initial labeling studies using purified protein, the PH domain of Akt, in which probes were found to label this target, as judged by on-gel detection. Furthermore, protein labeling was abrogated by controls including competition with an unlabeled PI(3,4,5)P3 headgroup analog as well as through protein denaturation, indicating specific labeling. In addition, probes featuring different linker lengths between the PI(3,4,5)P3 headgroup and photoaffinity tag led to variations in protein labeling, indicating that a shorter linker was more effective in this case. Finally, proteomic labeling studies were performed using cell extracts, labeled proteins were observed by in-gel detection and characterized using post-labeling with biotin, affinity chromatography and identification via tandem mass spectrometry. These studies yielded a total of 265 proteins

  1. Tin-117m-labeled stannic (Sn.sup.4+) chelates

    DOEpatents

    Srivastava, Suresh C.; Meinken, George E.; Richards, Powell

    1985-01-01

    The radiopharmaceutical reagents of this invention and the class of Tin-117m radiopharmaceuticals are therapeutic and diagnostic agents that incorporate gamma-emitting nuclides that localize in bone after intravenous injection in mammals (mice, rats, dogs, and rabbits). Images reflecting bone structure or function can then be obtained by a scintillation camera that detects the distribution of ionizing radiation emitted by the radioactive agent. Tin-117m-labeled chelates of stannic tin localize almost exclusively in cortical bone. Upon intravenous injection of the reagent, the preferred chelates are phosphonate compounds, preferable, PYP, MDP, EHDP, and DTPA. This class of reagents is therapeutically and diagnostically useful in skeletal scintigraphy and for the radiotherapy of bone tumors and other disorders.

  2. Radiosensitivity of Prostate Cancer Cell Lines for Irradiation from Beta Particle-emitting Radionuclide ¹⁷⁷Lu Compared to Alpha Particles and Gamma Rays.

    PubMed

    Elgqvist, Jörgen; Timmermand, Oskar Vilhelmsson; Larsson, Erik; Strand, Sven-Erik

    2016-01-01

    The purpose of the present study was to investigate the radiosensitivity of the prostate cancer cell lines LNCaP, DU145, and PC3 when irradiated with beta particles emitted from (177)Lu, and to compare the effect with irradiation using alpha particles or gamma rays. Cells were irradiated with beta particles emitted from (177)Lu, alpha particles from (241)Am, or gamma rays from (137)Cs. A non-specific polyclonal antibody was labeled with (177)Lu and used to irradiate cells in suspension with beta particles. A previously described in-house developed alpha-particle irradiator based on a (241)Am source was used to irradiate cells with alpha particles. External gamma-ray irradiation was achieved using a standard (137)Cs irradiator. Cells were irradiated to absorbed doses equal to 0, 0.5, 1, 2, 4, 6, 8, or 10 Gy. The absorbed doses were calculated as mean absorbed doses. For evaluation of cell survival, the tetrazolium-based WST-1 assay was used. After irradiation, WST-1 was added to the cell solutions, incubated, and then measured for level of absorbance at 450 nm, indicating the live and viable cells. LNCaP, DU145, and PC3 cell lines all had similar patterns of survival for the different radiation types. No significant difference in surviving fractions were observed between cells treated with beta-particle and gamma-ray irradiation, represented for example by the surviving fraction values (mean±SD) at 2, 6, and 10 Gy (SF2, SF6, and SF10) for DU145 after beta-particle irradiation: 0.700±0.090, 0.186±0.050 and 0.056±0.010, respectively. A strong radiosensitivity to alpha particles was observed, with SF2 values of 0.048±0.008, 0.018±0.006 and 0.015±0.005 for LNCaP, DU145, and PC3, respectively. The surviving fractions after irradiation using beta particles or gamma rays did not differ significantly at the absorbed dose levels and dose rates used. Irradiation using alpha particles led to a high level of cell killing. The results show that the beta-particle emitter

  3. VHE gamma-ray Emitting Pulsar Wind Nebulae Discovered by H.E.S.S.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gallant, Y.A.; /Montpellier U.; Carrigan, S.

    2008-06-05

    Recent advances in very-high-energy (VHE) gamma-ray astronomy have opened a new observational window on the physics of pulsars. The high sensitivity of current imaging atmospheric Cherenkov telescopes, and in particular of the H.E.S.S. array, has already led to the discovery of about a dozen VHE-emitting pulsar wind nebulae (PWNe) and PWN candidates. These include the plerions in the composite supernova remnants MSH 15-52, G21.5-0.9, Kes 75, and Vela, two sources in the Kookaburra, and the nebula of PSR B1823-13. This VHE emission is generally interpreted as inverse Compton emission from the relativistic electrons and positrons accelerated by the pulsar andmore » its wind; as such, it can yield a more direct spatial and spectral view of the accelerated particles than can be inferred from observations of their synchrotron emission. The VHE-emitting PWNe detected by the H.E.S.S. telescopes are reviewed and the implications for pulsar physics discussed.« less

  4. Detecting special nuclear materials in containers using high-energy gamma rays emitted by fission products

    DOEpatents

    Norman, Eric B.; Prussin, Stanley G.

    2007-10-02

    A method and a system for detecting the presence of special nuclear materials in a container. The system and its method include irradiating the container with an energetic beam, so as to induce a fission in the special nuclear materials, detecting the gamma rays that are emitted from the fission products formed by the fission, to produce a detector signal, comparing the detector signal with a threshold value to form a comparison, and detecting the presence of the special nuclear materials using the comparison.

  5. Material efficiency studies for a Compton camera designed to measure characteristic prompt gamma rays emitted during proton beam radiotherapy

    PubMed Central

    Robertson, Daniel; Polf, Jerimy C; Peterson, Steve W; Gillin, Michael T; Beddar, Sam

    2011-01-01

    Prompt gamma rays emitted from biological tissues during proton irradiation carry dosimetric and spectroscopic information that can assist with treatment verification and provide an indication of the biological response of the irradiated tissues. Compton cameras are capable of determining the origin and energy of gamma rays. However, prompt gamma monitoring during proton therapy requires new Compton camera designs that perform well at the high gamma energies produced when tissues are bombarded with therapeutic protons. In this study we optimize the materials and geometry of a three-stage Compton camera for prompt gamma detection and calculate the theoretical efficiency of such a detector. The materials evaluated in this study include germanium, bismuth germanate (BGO), NaI, xenon, silicon and lanthanum bromide (LaBr3). For each material, the dimensions of each detector stage were optimized to produce the maximum number of relevant interactions. These results were used to predict the efficiency of various multi-material cameras. The theoretical detection efficiencies of the most promising multi-material cameras were then calculated for the photons emitted from a tissue-equivalent phantom irradiated by therapeutic proton beams ranging from 50 to 250 MeV. The optimized detector stages had a lateral extent of 10 × 10 cm2 with the thickness of the initial two stages dependent on the detector material. The thickness of the third stage was fixed at 10 cm regardless of material. The most efficient single-material cameras were composed of germanium (3 cm) and BGO (2.5 cm). These cameras exhibited efficiencies of 1.15 × 10−4 and 9.58 × 10−5 per incident proton, respectively. The most efficient multi-material camera design consisted of two initial stages of germanium (3 cm) and a final stage of BGO, resulting in a theoretical efficiency of 1.26 × 10−4 per incident proton. PMID:21508442

  6. Measurement and analysis of gamma-rays emitted from spent nuclear fuel above 3 MeV.

    PubMed

    Rodriguez, Douglas C; Anderson, Elaina; Anderson, Kevin K; Campbell, Luke W; Fast, James E; Jarman, Kenneth; Kulisek, Jonathan; Orton, Christopher R; Runkle, Robert C; Stave, Sean

    2013-12-01

    The gamma-ray spectrum of spent nuclear fuel in the 3-6 MeV energy range is important for active interrogation since gamma rays emitted from nuclear decay are not expected to interfere with measurements in this energy region. There is, unfortunately, a dearth of empirical measurements from spent nuclear fuel in this region. This work is an initial attempt to partially fill this gap by presenting an analysis of gamma-ray spectra collected from a set of spent nuclear fuel sources using a high-purity germanium detector array. This multi-crystal array possesses a large collection volume, providing high energy resolution up to 16 MeV. The results of these measurements establish the continuum count-rate in the energy region between 3 and 6 MeV. Also assessed is the potential for peaks from passive emissions to interfere with peak measurements resulting from active interrogation delayed emissions. As one of the first documented empirical measurements of passive emissions from spent fuel for energies above 3 MeV, this work provides a foundation for active interrogation model validation and detector development. © 2013 Elsevier Ltd. All rights reserved.

  7. Apparatus and method for reading two-dimensional electrophoretograms containing .beta.-ray-emitting labeled compounds

    DOEpatents

    Anderson, Herbert L.; Kinnison, W. Wayne; Lillberg, John W.

    1987-01-01

    Apparatus and method for electronically reading planar two dimensional .beta.-ray emitter-labeled gel electrophoretograms. A single, flat rectangular multiwire proportional chamber is placed in close proximity to the gel and the assembly placed in an intense uniform magnetic field disposed in a perpendicular manner to the rectangular face of the proportional chamber. Beta rays emitted in the direction of the proportional chamber are caused to execute helical motions which substantially preserve knowledge of the coordinates of their origin in the gel. Perpendicularly oriented, parallel wire, parallel plane cathodes electronically sense the location of the .beta.-rays from ionization generated thereby in a detection gas coupled with an electron avalanche effect resulting from the action of a parallel wire anode located therebetween. A scintillator permits the present apparatus to be rendered insensitive when signals are generated from cosmic rays incident on the proportional chamber. Resolution for concentrations of radioactive compounds in the gel exceeds 700 .mu.m. The apparatus and method of the present invention represent a significant improvement over conventional autoradiographic techniques in dynamic range, linearity and sensitivity of data collection. A concentration and position map for gel electrophoretograms having significant concentrations of labeled compounds and/or highly radioactive labeling nuclides can generally be obtained in less than one hour.

  8. Cross-linking of hCG to luteal receptors

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ji, T.H.; Ji, I.

    1985-01-01

    Photoaffinity labeling of the lutropin/choriogonadotropin (LH/hCG) receptor system on porcine granulosa cells has demonstrated that both the ..cap alpha.. and ..beta.. subunits of hCG directly photoaffinity label the hormone receptor. Three new bands appear on SDS-PAGE as a consequence of photoaffinity labeling by each subunit: the molecular weights of the three bands (106K, 88K, and 83K) produced by the subunit are larger by approximately 10K than those of the three bands (96K, 76K, and 73K) labeled by the ..cap alpha.. subunit. Although it could be a coincidence that the molecular weight of the ..beta.. subunit is approximately 10K larger thanmore » that of the ..cap alpha.. subunit, the similarity in these differences suggests the possibility that both the ..cap alpha.. and ..beta.. subunits have labeled the same polypeptides.« less

  9. A photoaffinity ligand for dopamine D2 receptors: azidoclebopride.

    PubMed

    Niznik, H B; Guan, J H; Neumeyer, J L; Seeman, P

    1985-02-01

    In order to label D2 dopamine receptors selectively and covalently by means of a photosensitive compound, azidoclebopride was synthesized directly from clebopride. The dissociation constant (KD) of clebopride for the D2 dopamine receptor (canine brain striatum) was 1.5 nM, while that for azidoclebopride was 21 nM. The affinities of both clebopride and azidoclebopride were markedly reduced in the absence of sodium chloride. In the presence of ultraviolet light, azidoclebopride inactivated D2 dopamine receptors irreversibly, as indicated by the inability of the receptors to bind [3H]spiperone. Maximal photoinactivation of about 60% of the D2 dopamine receptors occurred at 1 microM azidoclebopride; 30% of the receptors were inactivated at 80 nM azidoclebopride (pseudo-IC50). Dopamine agonists selectively protected the D2 receptors from being inactivated by azidoclebopride, the order of potency being (-)-N-n-propylnorapomorphine greater than apomorphine greater than (+/-)-6,7-dihydroxy-2-aminotetralin greater than (+)-N-n-propylnorapomorphine greater than dopamine greater than noradrenaline greater than serotonin. Similarly, dopaminergic antagonists prevented the photoinactivation of D2 receptors by azidoclebopride with the following order of potency: spiperone greater than (+)-butaclamol greater than haloperidol greater than clebopride greater than (-)-sulpiride greater than (-)-butaclamol. The degree of D2 dopamine receptor photoinduced inactivation by azidoclebopride was not significantly affected by scavengers such as p-aminobenzoic acid and dithiothreitol. Furthermore, irradiation of striatal membranes with a concentration of azidoclebopride sufficient to inactivate dopamine D2 receptors by 60% did not significantly reduce dopamine D1, serotonin (S2), benzodiazepine, alpha 1- or beta-noradrenergic receptors. This study describes the use of a novel and selective photoaffinity ligand for brain dopamine D2 receptors. The molecule, in radiolabeled form, may aid in the

  10. Detecting special nuclear materials in suspect containers using high-energy gamma rays emitted by fission products

    DOEpatents

    Norman, Eric B [Oakland, CA; Prussin, Stanley G [Kensington, CA

    2009-05-05

    A method and a system for detecting the presence of special nuclear materials in a suspect container. The system and its method include irradiating the suspect container with a beam of neutrons, so as to induce a thermal fission in a portion of the special nuclear materials, detecting the gamma rays that are emitted from the fission products formed by the thermal fission, to produce a detector signal, comparing the detector signal with a threshold value to form a comparison, and detecting the presence of the special nuclear materials using the comparison.

  11. Detecting special nuclear materials in suspect containers using high-energy gamma rays emitted by fission products

    DOEpatents

    Norman, Eric B [Oakland, CA; Prussin, Stanley G [Kensington, CA

    2009-01-27

    A method and a system for detecting the presence of special nuclear materials in a suspect container. The system and its method include irradiating the suspect container with a beam of neutrons, so as to induce a thermal fission in a portion of the special nuclear materials, detecting the gamma rays that are emitted from the fission products formed by the thermal fission, to produce a detector signal, comparing the detector signal with a threshold value to form a comparison, and detecting the presence of the special nuclear materials using the comparison.

  12. Detecting special nuclear materials in suspect containers using high-energy gamma rays emitted by fission products

    DOEpatents

    Norman, Eric B [Oakland, CA; Prussin, Stanley G [Kensington, CA

    2009-01-06

    A method and a system for detecting the presence of special nuclear materials in a suspect container. The system and its method include irradiating the suspect container with a beam of neutrons, so as to induce a thermal fission in a portion of the special nuclear materials, detecting the gamma rays that are emitted from the fission products formed by the thermal fission, to produce a detector signal, comparing the detector signal with a threshold value to form a comparison, and detecting the presence of the special nuclear materials using the comparison.

  13. Label free biosensor incorporating a replica-molded, vertically emitting distributed feedback laser

    NASA Astrophysics Data System (ADS)

    Lu, M.; Choi, S. S.; Wagner, C. J.; Eden, J. G.; Cunningham, B. T.

    2008-06-01

    A label free biosensor based upon a vertically emitting distributed feedback (DFB) laser has been demonstrated. The DFB laser comprises a replica-molded, one-dimensional dielectric grating coated with laser dye-doped polymer as the gain medium. Adsorption of biomolecules onto the laser surface alters the DFB laser emission wavelength, thereby permitting the kinetic adsorption of a protein polymer monolayer or the specific binding of small molecules to be quantified. A bulk sensitivity of 16.6nm per refractive index unit and the detection of a monolayer of the protein polymer poly(Lys, Phe) have been observed with this biosensor. The sensor represents a departure from conventional passive resonant optical sensors from the standpoint that the device actively generates its own narrowband high intensity output without stringent requirements on the coupling alignments, resulting in a simple, robust illumination and detection configuration.

  14. Biodistribution and catabolism of 18F-labelled isopeptide N(epsilon)-(gamma-glutamyl)-L-lysine.

    PubMed

    Hultsch, C; Bergmann, R; Pawelke, B; Pietzsch, J; Wuest, F; Johannsen, B; Henle, T

    2005-12-01

    Isopeptide bonds between the epsilon-amino group of lysine and the gamma-carboxamide group of glutamine are formed during strong heating of pure proteins or, more important, by enzymatic reaction mediated by transglutaminases. Despite the wide use of a microbial transglutaminase in food biotechnology, up to now little is known about the metabolic fate of the isopeptide N(epsilon)-(gamma-glutamyl)-L-lysine. In the present study, N-succinimidyl-4-[(18)F]fluorobenzoate was used to modify N(epsilon)-(gamma-glutamyl)-L-lysine at each of its two alpha-amino groups, resulting in the 4-[(18)F]fluorobenzoylated derivatives, for which biodistribution, catabolism, and elimination were investigated in male Wistar rats. A significant different biochemical behavior of the two labelled isopeptides was observed in terms of in vitro stability, in vivo metabolism as well as biodistribution. The results suggest that the metabolic fate of isopeptides is likely to be dependent on how they are reabsorbed - free or peptide bound.

  15. Sneaky Gamma-Rays: Using Gravitational Lensing to Avoid Gamma-Gamma-Absorption

    NASA Astrophysics Data System (ADS)

    Boettcher, Markus; Barnacka, Anna

    2014-08-01

    It has recently been suggested that gravitational lensing studies of gamma-ray blazars might be a promising avenue to probe the location of the gamma-ray emitting region in blazars. Motivated by these prospects, we have investigated potential gamma-gamma absorption signatures of intervening lenses in the very-high-energy gamma-ray emission from lensedblazars. We considered intervening galaxies and individual stars within these galaxies. We find that the collective radiation field of galaxies acting as sources of macrolensing are not expected to lead to significant gamma-gamma absorption. Individual stars within intervening galaxies could, in principle, cause a significant opacity to gamma-gamma absorption for VHE gamma-rays if the impact parameter (the distance of closest approach of the gamma-ray to the center of the star) is small enough. However, we find that the curvature of the photon path due to gravitational lensing will cause gamma-ray photons to maintain a sufficiently large distance from such stars to avoid significant gamma-gamma absorption. This re-inforces the prospect of gravitational-lensing studies of gamma-ray blazars without interference due to gamma-gamma absorption due to the lensing objects.

  16. /sup 3/H)forskolin. Direct photoaffinity labeling of the erythrocyte D-glucose transporter

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shanahan, M.F.; Morris, D.P.; Edwards, B.M.

    1987-05-05

    Irradiation of erythrocyte ghosts in the presence of (/sup 3/H)forskolin resulted in a concentration-dependent, covalent incorporation of radiolabel into several of the major membrane protein bands. Most of the incorporation occurred in four regions of the gel. Peak 1 (216 kDa) was a sharp peak near the top of the gel in the region corresponding to spectrin. Peak 2 appeared to be associated with band 3 (89 kDa), while a third peak occurred around the position of band 4.2 (76 kDa). The fourth region of labeling was a broad area between 43-75 kDa which corresponds to the region of themore » glucose transporter. Forskolin labeling of this region was inhibited by cytochalasin B and D-glucose, but not L-glucose. Extraction of extrinsic membrane proteins resulted in a loss of radiolabeled protein from the 216- and 76-kDa regions. Treatment of membranes labeled with either cytochalasin B or forskolin with endo-beta-galactosidase resulted in identical shifts of the 43 to 75-kDa peaks to 42 kDa. Similarly, trypsinization of membranes photolabeled with either cytochalasin B or forskolin resulted in the generation of a 17-kDa radiolabeled fragment in both cases. Photoincorporation of (/sup 3/H)cytochalasin B into the glucose transporter was blocked in a concentration-dependent manner by unlabeled forskolin.« less

  17. Gamma ray pulsars

    NASA Technical Reports Server (NTRS)

    Oegelman, H.; Ayasli, S.; Hacinliyan, A.

    1976-01-01

    Recent data from the high energy gamma ray experiment have revealed the existence of four pulsars emitting photons above 35 MeV. An attempt is made to explain the gamma ray emission from these pulsars in terms of an electron-photon cascade that develops in the magnetosphere of the pulsar. Although there is very little material above the surface of the pulsar, the very intense magnetic fields correspond to many radiation lengths which cause electrons to emit photons via magnetic bremsstrahlung and these photons to pair produce. The cascade develops until the mean photon energy drops below the pair production threshold which happens to be in the gamma ray range; at this stage the photons break out from the source.

  18. Inter-laboratory comparisons of short-lived gamma-emitting radionuclides in nuclear reactor water.

    PubMed

    Klemola, S K

    2008-01-01

    Inter-laboratory comparisons of gamma-emitting nuclides in nuclear power plant coolant water have been carried out in Finland since 1994. The reactor water samples are taken and prepared by one of the two nuclear power plants and delivered to the participants. Since all the participants get their sample within just a few hours it has been possible to analyse and compare results of nuclides with half-lives shorter than 1h. The total number of short-lived nuclides is 26. All the main nuclides are regularly identified and the activities have been obtained with reasonable accuracy throughout the years. The overall deviation of the results has decreased in 13 years. The effects of true coincidence summing and discrepancies in nuclear data have been identified as potential sources of remaining discrepancies. All the participants have found this type of comparison very useful.

  19. Estimation of neutron energy distributions from prompt gamma emissions

    NASA Astrophysics Data System (ADS)

    Panikkath, Priyada; Udupi, Ashwini; Sarkar, P. K.

    2017-11-01

    A technique of estimating the incident neutron energy distribution from emitted prompt gamma intensities from a system exposed to neutrons is presented. The emitted prompt gamma intensities or the measured photo peaks in a gamma detector are related to the incident neutron energy distribution through a convolution of the response of the system generating the prompt gammas to mono-energetic neutrons. Presently, the system studied is a cylinder of high density polyethylene (HDPE) placed inside another cylinder of borated HDPE (BHDPE) having an outer Pb-cover and exposed to neutrons. The emitted five prompt gamma peaks from hydrogen, boron, carbon and lead can be utilized to unfold the incident neutron energy distribution as an under-determined deconvolution problem. Such an under-determined set of equations are solved using the genetic algorithm based Monte Carlo de-convolution code GAMCD. Feasibility of the proposed technique is demonstrated theoretically using the Monte Carlo calculated response matrix and intensities of emitted prompt gammas from the Pb-covered BHDPE-HDPE system in the case of several incident neutron spectra spanning different energy ranges.

  20. Identification of a receptor for ADP on blood platelets by photoaffinity labelling.

    PubMed Central

    Cristalli, G; Mills, D C

    1993-01-01

    The synthesis of a new analogue of ADP, 2-(p-azidophenyl)-ethythioadenosine 5'-diphosphate (AzPET-ADP), is described. This compound contains a photolabile phenylazide group attached to the ADP molecule by a thioether link at the purine 2 position. It has been prepared in radioactive form with 32P in the beta-phosphate at a specific radioactivity of 100 mCi/mumol. The reagent activated platelets, causing shape change and aggregation, with somewhat lower affinity than ADP. On photolysis the affinity was increased. The reagent also inhibited platelet adenylate cyclase stimulation by prostaglandin E1, with considerably higher affinity than ADP. On photolysis the affinity was decreased. AzPET-ADP competitively inhibited the binding of 2-methylthio[beta-32P]ADP, a ligand for the receptor by which ADP causes inhibition of adenylate cyclase. In the dark, AzPET-[beta-32P]ADP bound reversibly and with high affinity to a single population of sites similar in number to the sites that bind 2-methylthio[beta-32P]ADP. Binding was inhibited by ADP and by ATP and by p-chloromercuribenzenesulphonic acid (pCMBS). On exposure to u.v. light in the presence of platelets, AzPET-[beta-32P]ADP was incorporated covalently but non-specifically into several platelet proteins, although prominent intracellular proteins were not labelled. Specific labelling was confined to a single region of SDS/polyacrylamide gels, overlying but not comigrating with actin. Incorporation of radioactivity into this region was inhibited by ADP and by ATP as well as by ADP beta S, ATP alpha S and pCMBS, but not by adenosine, GDP or AMP. Inhibition of AzPET-[beta-32P]ADP incorporation was closely correlated with inhibition of equilibrium binding of 2-methylthio[beta-32P]ADP. These results suggests that the labelled protein, which migrates with an apparent molecular mass of 43 kDa in reduced gels, is the receptor through which ADP inhibits adenylate cyclase. Images Figure 5 PMID:8387782

  1. One-dimensional Spatial Distributions of Gamma-ray Emitting Contaminants in Field Lysimeters Using a Collimated Gamma-ray Spectroscopy System.

    PubMed

    Erdmann, Bryan J; Powell, Brian A; Kaplan, Daniel I; DeVol, Timothy A

    2018-05-01

    One-dimensional scans of gamma-ray emitting contaminants were conducted on lysimeters from the RadFLEX facility at the Savannah River Nationals Laboratory (SRNL). The lysimeters each contained a contamination source that was buried in SRNL soil. A source consisted of Cs, Co, Ba, and Eu incorporated either into a solid waste form (Portland cement and reducing grout) or applied to a filter paper for direct soil exposure. The lysimeters were exposed to natural environmental conditions for 3 to 4 y. The initial contaminant activities range from 4.0 to 9.0 MBq for the solid wasteforms and 0.25 to 0.47 MBq for the soil-incorporated source. The measurements were performed using a collimated high-purity germanium gamma-ray spectrometer with a spatial resolution of 2.5 mm. These scans showed downward mobility of Co and Ba when the radionuclides were incorporated directly into the SRNL soil. When radionuclides were incorporated into the solid waste forms positioned in the SRNL soil, Cs exhibited both upward and downward dispersion while the other radionuclides showed no movement. This dispersion was more significant for the Portland cement than the reducing grout wasteform. Europium-152 was the only radionuclide of those studied that showed no movement within the spatial resolution of the scanner from the original placement within the lysimeter. Understanding radionuclide movement in the environment is important for developing strategies for waste management and disposal.

  2. Sediment studies at Bikini Atoll part 3. Inventories of some long-lived gamma-emitting radionuclides associated with lagoon surface sediments

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Noshkin, V.E.

    1997-12-01

    Surface sediment samples were collected during 1979 from 87 locations in the lagoon at Bikini Atoll. The collections were made to better define the concentrations and distribution of long-lived radionuclides associated with the bottom material and to show what modifications occurred to the composition of the surface sediment from the nuclear testing program conducted by the United States at the Atoll between 1946 and 1958. This is the last of three reports on Bikini sediment studies. In this report, we discuss the concentrations and inventories of the residual long-lived gamma-emitting radionuclides in sediments from the lagoon. The gamma-emitting radionuclides detectedmore » most frequently in sediments collected in 1979, in addition to Americium-241 ({sup 241}Am) (discussed in the second report of this series), included Cesium-137 ({sup 137}Cs), Bismuth-207 ({sup 207}Bi), Europium-155 ({sup 155}Eu), and Cobalt-60 ({sup 60}Co). Other man-made, gamma-emitting radionuclides such as Europium-152,154 ({sup 152,154}Eu), Antimony-125 ({sup 125}Sb), and Rhodium-101,102m ({sup 101,102m}Rh) were occasionally measured above detection limits in sediments near test site locations. The mean inventories for {sup 137}Cs, {sup 207}Ei, {sup 155}Eu, and {sup 60}Co in the surface 4 cm of the lagoon sediment to be 1.7, 0.56, 7.76, and 0.74 TBq, respectively. By June 1997, radioactive decay would reduce these values to 1.1, 0.38, 0.62, and 0.07 TBq, respectively. Some additional loss results from a combination of different processes that continuously mobilize and return some amount of the radionuclides to the water column. The water and dissolved constituents are removed from the lagoon through channels and exchange with the surface waters of the north equatorial Pacific Ocean. Highest levels of these radionuclides are found in surface deposits lagoonward of the Bravo Crater. Lowest concentrations and inventories are associated with sediment lagoonward of the eastern reef. The

  3. 18F-positron-emitting/fluorescent labeled erythrocytes allow imaging of internal hemorrhage in a murine intracranial hemorrhage model

    PubMed Central

    Wang, Ye; An, Fei-Fei; Chan, Mark; Friedman, Beth; Rodriguez, Erik A; Tsien, Roger Y; Aras, Omer

    2017-01-01

    An agent for visualizing cells by positron emission tomography is described and used to label red blood cells. The labeled red blood cells are injected systemically so that intracranial hemorrhage can be visualized by positron emission tomography (PET). Red blood cells are labeled with 0.3 µg of a positron-emitting, fluorescent multimodal imaging probe, and used to non-invasively image cryolesion induced intracranial hemorrhage in a murine model (BALB/c, 2.36 × 108 cells, 100 µCi, <4 mm hemorrhage). Intracranial hemorrhage is confirmed by histology, fluorescence, bright-field, and PET ex vivo imaging. The low required activity, minimal mass, and high resolution of this technique make this strategy an attractive alternative for imaging intracranial hemorrhage. PET is one solution to a spectrum of issues that complicate single photon emission computed tomography (SPECT). For this reason, this application serves as a PET alternative to [99mTc]-agents, and SPECT technology that is used in 2 million annual medical procedures. PET contrast is also superior to gadolinium and iodide contrast angiography for its lack of clinical contraindications. PMID:28054494

  4. Gamma-emitting radionuclides in the shallow marine sediments off the Sindh coast, Arabian Sea.

    PubMed

    Akram, M; Qureshi, Riffat M; Ahmad, Nasir; Solaija, Tariq Jamal

    2006-01-01

    Determination of gamma emitting radionuclides in shallow marine sediments off the Sindh coast has been carried out using a gamma spectrometry technique. The activity concentration measured in various sediment samples off the Sindh coast has been found to vary from 15.93 +/- 5.22 to 30.53 +/- 4.70 Bq kg(-1) for 226Ra, from 11.72 +/- 1.22 to 33.94 +/- 1.86 Bq kg(-1) for 228Ra and from 295.22 +/- 32.83 to 748.47 +/- 28.75 Bq kg(-1) for 40K. The calculated mean values of radium equivalent activity, absorbed dose rate and effective dose are 98 Bq kg(-1), 49 nGy h(-1) and 0.06 mSv y(-1), respectively. No artificial radionuclide was detected in the samples measured from the study area. As no data on radioactivity of the coastal environment of Pakistan are available, the data presented here will serve as baseline information on radionuclide concentration in shallow sea sediments off the Sindh coast. The data will also be useful for tracking pollution inventories from unusual radiological events (if any) in the territorial waters of the study area. Further, the information presented will contribute to modelling of a regional radioactivity database from the perspectives of the International Atomic Energy Agency's Asia-Pacific Marine Radioactivity Database and Global Marine Radioactivity Database.

  5. Tin-117m-labeled stannic (Sn/sup 4 +/) chelate of diethylenetriamine pentaacetic acid (DTPA) for application in diagnosis and therapy

    DOEpatents

    Srivastava, S.C.; Meinken, G.E.; Richards, P.

    1983-08-25

    The radiopharmaceutical reagents of this invention and the class of Tin-117m radiopharmaceuticals are therapeutic and diagnostic agents that incorporate gamma-emitting nuclides that localize in bone after intravenous injection in mammals (mice, rats, dogs, and rabbits). Images reflecting bone structure or function can then be obtained by a scintillation camera that detects the distribution of ionizing radiation emitted by the radioactive agent. Tin-117m-labeled chelates of stannic tin localize almost exclusively in cortical bone. Upon intravenous injection of the reagent, the preferred chelates are phosphonate compounds, preferable, PYP, MDP, EHDP, and DTPA. This class of reagents is therapeutically and diagnostically useful in skeletal scintigraphy and for the radiotherapy of bone tumors and other disorders.

  6. Photoaffinity labeling of the Torpedo californica nicotinic acetylcholine receptor with an aryl azide derivative of phosphatidylserine

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Blanton, M.P.; Wang, H.H.

    1990-02-06

    A photoactivatable analogue of phosphatidylserine, {sup 125}I-labeled 4-azidosalicylic acid-phosphatidylserine ({sup 125}I ASA-PS), was used to label both native acetylcholine receptor (AchR)-rich membranes from Torpedo californica and AchR membranes affinity purified from Torpedo reconstituted into asolectin vesicles. The radioiodinated arylazido group attaches directly to the phospholipid head group and thus probes for regions of the AchR structure in contact with the negatively charged head group of phosphatidylserine. All four subunits of the AchR incorporated the label, with the {alpha} subunit incorporating approximately twice as much as each of the other subunits on a per mole basis. The regions of the AchRmore » {alpha} subunit that incorporated {sup 125}I ASA-PS were mapped by Staphylococcus aureus V8 protease digestion. The majority of label incorporated into fragments representing a more complete digestion of the {alpha} subunit was localized to 11.7- and 10.1-kDa V8 cleavage fragments, both beginning at Asn-339 and of sufficient length to contain the hydrophobic region M4. An 18.7-kDa fragment beginning at Ser-173 and of sufficient length to contain the hydrophobic regions M1, M2, and M3 was also significantly labeled. In contrast, V8 cleavage fragments representing roughly a third of the amino-terminal portion of the {alpha} subunit incorporated little or no detectable amount of probe.« less

  7. Spatial segregation of gamma-secretase and substrates in distinct membrane domains.

    PubMed

    Vetrivel, Kulandaivelu S; Cheng, Haipeng; Kim, Seong-Hun; Chen, Ying; Barnes, Natalie Y; Parent, Angèle T; Sisodia, Sangram S; Thinakaran, Gopal

    2005-07-08

    Gamma-secretase facilitates the regulated intramembrane proteolysis of select type I membrane proteins that play diverse physiological roles in multiple cell types and tissue. In this study, we used biochemical approaches to examine the distribution of amyloid precursor protein (APP) and several additional gamma-secretase substrates in membrane microdomains. We report that APP C-terminal fragments (CTFs) and gamma-secretase reside in Lubrol WX detergent-insoluble membranes (DIM) of cultured cells and adult mouse brain. APP CTFs that accumulate in cells lacking gamma-secretase activity preferentially associate with DIM. Cholesterol depletion and magnetic immunoisolation studies indicate recruitment of APP CTFs into cholesterol- and sphingolipid-rich lipid rafts, and co-residence of APP CTFs, PS1, and syntaxin 6 in DIM patches derived from the trans-Golgi network. Photoaffinity cross-linking studies provided evidence for the preponderance of active gamma-secretase in lipid rafts of cultured cells and adult brain. Remarkably, unlike the case of APP, CTFs derived from Notch1, Jagged2, deleted in colorectal cancer (DCC), and N-cadherin remain largely detergent-soluble, indicative of their spatial segregation in non-raft domains. In embryonic brain, the majority of PS1 and nicastrin is present in Lubrol WX-soluble membranes, wherein the CTFs derived from APP, Notch1, DCC, and N-cadherin also reside. We suggest that gamma-secretase residence in non-raft membranes facilitates proteolysis of diverse substrates during embryonic development but that the translocation of gamma-secretase to lipid rafts in adults ensures processing of certain substrates, including APP CTFs, while limiting processing of other potential substrates.

  8. Development of Simultaneous Beta-and-Coincidence-Gamma Imager for Plant Imaging Research

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tai, Yuan-Chuan

    2016-09-30

    The goal of this project is to develop a novel imaging system that can simultaneously acquire beta and coincidence gamma images of positron sources in thin objects such as leaves of plants. This hybrid imager can be used to measure carbon assimilation in plants quantitatively and in real-time after C-11 labeled carbon-dioxide is administered. A better understanding of carbon assimilation, particularly under the increasingly elevated atmospheric CO 2 level, is extremely critical for plant scientists who study food crop and biofuel production. Phase 1 of this project is focused on the technology development with 3 specific aims: (1) develop amore » hybrid detector that can detect beta and gamma rays simultaneously; (2) develop an imaging system that can differentiate these two types of radiation and acquire beta and coincidence gamma images in real-time; (3) develop techniques to quantify radiotracer distribution using beta and gamma images. Phase 2 of this project is to apply technologies developed in phase 1 to study plants using positron-emitting radionuclide such as 11C to study carbon assimilation in biofuel plants.« less

  9. Distinct metabolites for photoreactive L-phenylalanine derivatives in Klebsiella sp. CK6 isolated from rhizosphere of a wild dipterocarp sapling.

    PubMed

    Wang, Lei; Hisano, Wataru; Murai, Yuta; Sakurai, Munenori; Muto, Yasuyuki; Ikemoto, Haruka; Okamoto, Masashi; Murotani, Takashi; Isoda, Reika; Kim, Dongyeop; Sakihama, Yasuko; Sitepu, Irnayuli R; Hashidoko, Yasuyuki; Hatanaka, Yasumaru; Hashimoto, Makoto

    2013-07-16

    Photoaffinity labeling is a reliable analytical method for biological functional analysis. Three major photophores--aryl azide, benzophenone and trifluoromethyldiazirine--are utilized in analysis. Photophore-bearing L-phenylalanine derivatives, which are used for biological functional analysis, were inoculated into a Klebsiella sp. isolated from the rhizosphere of a wild dipterocarp sapling in Central Kalimantan, Indonesia, under nitrogen-limiting conditions. The proportions of metabolites were quite distinct for each photophore. These results indicated that photophores affected substrate recognition in rhizobacterial metabolic pathways, and differential photoaffinity labeling could be achieved using different photophore-containing L-phenylalanine derivatives.

  10. [An investigation of the effect of gamma rays emitted by patients undergoing radionuclide bone scintigraphy in computed radiography during X-ray mammography examination].

    PubMed

    Kamigiri, Akira; Nagasawa, Naoki; Yamaji, Masami; Nakamura, Mikako; Ito, Morihiro; Nakanishi, Satoshi; Kitano, Tokio

    2010-03-20

    For convenience of outpatients, mammographies of outpatients are often taken after the injection of a radionuclide. In this study, we investigated the effects of gamma rays emitted by a patient onto imaging plates (IPs). We used a flat container filled with (99m)Tc solution as a planar source to irradiate gamma rays onto IPs. We changed irradiation times on each IP, and took radiographies of an ACR-specified 156 model phantom and AGH-D210F phantom. We evaluated radiography images, using visual evaluation, and profile curves, histograms, and CNR and RMS granularities analyses. The results indicated that the depiction ability of a fibrous part began to fall when the irradiation time exceeded 3 minutes. With an increase in irradiation time, an increase in pixel value and RMS granularity value and a decrease in CNR value were observed. In conclusion, IP exposed by gamma rays influenced the evaluation of phantom images.

  11. Detection of Only Viable Bacterial Spores Using a Live/Dead Indicator in Mixed Populations

    NASA Technical Reports Server (NTRS)

    Behar, Alberto E.; Stam, Christina N.; Smiley, Ronald

    2013-01-01

    This method uses a photoaffinity label that recognizes DNA and can be used to distinguish populations of bacterial cells from bacterial spores without the use of heat shocking during conventional culture, and live from dead bacterial spores using molecular-based methods. Biological validation of commercial sterility using traditional and alternative technologies remains challenging. Recovery of viable spores is cumbersome, as the process requires substantial incubation time, and the extended time to results limits the ability to quickly evaluate the efficacy of existing technologies. Nucleic acid amplification approaches such as PCR (polymerase chain reaction) have shown promise for improving time to detection for a wide range of applications. Recent real-time PCR methods are particularly promising, as these methods can be made at least semi-quantitative by correspondence to a standard curve. Nonetheless, PCR-based methods are rarely used for process validation, largely because the DNA from dead bacterial cells is highly stable and hence, DNA-based amplification methods fail to discriminate between live and inactivated microorganisms. Currently, no published method has been shown to effectively distinguish between live and dead bacterial spores. This technology uses a DNA binding photoaffinity label that can be used to distinguish between live and dead bacterial spores with detection limits ranging from 109 to 102 spores/mL. An environmental sample suspected of containing a mixture of live and dead vegetative cells and bacterial endospores is treated with a photoaffinity label. This step will eliminate any vegetative cells (live or dead) and dead endospores present in the sample. To further determine the bacterial spore viability, DNA is extracted from the spores and total population is quantified by real-time PCR. The current NASA standard assay takes 72 hours for results. Part of this procedure requires a heat shock step at 80 degC for 15 minutes before the

  12. Gamma ray pulsars. [electron-photon cascades

    NASA Technical Reports Server (NTRS)

    Oegelman, H.; Ayasli, S.; Hacinliyan, A.

    1977-01-01

    Data from the SAS-2 high-energy gamma-ray experiment reveal the existence of four pulsars emitting photons above 35 MeV. An attempt is made to explain the gamma-ray emission from these pulsars in terms of an electron-photon cascade that develops in the magnetosphere of the pulsar. Although there is very little material above the surface of the pulsar, the very intense magnetic fields (10 to the 12th power gauss) correspond to many radiation lengths which cause electrons to emit photons by magnetic bremsstrahlung and which cause these photons to pair-produce. The cascade develops until the mean photon energy drops below the pair-production threshold which is in the gamma-ray range; at this stage, the photons break out from the source.

  13. Detailed deposition density maps constructed by large-scale soil sampling for gamma-ray emitting radioactive nuclides from the Fukushima Dai-ichi Nuclear Power Plant accident.

    PubMed

    Saito, Kimiaki; Tanihata, Isao; Fujiwara, Mamoru; Saito, Takashi; Shimoura, Susumu; Otsuka, Takaharu; Onda, Yuichi; Hoshi, Masaharu; Ikeuchi, Yoshihiro; Takahashi, Fumiaki; Kinouchi, Nobuyuki; Saegusa, Jun; Seki, Akiyuki; Takemiya, Hiroshi; Shibata, Tokushi

    2015-01-01

    Soil deposition density maps of gamma-ray emitting radioactive nuclides from the Fukushima Dai-ichi Nuclear Power Plant (NPP) accident were constructed on the basis of results from large-scale soil sampling. In total 10,915 soil samples were collected at 2168 locations. Gamma rays emitted from the samples were measured by Ge detectors and analyzed using a reliable unified method. The determined radioactivity was corrected to that of June 14, 2011 by considering the intrinsic decay constant of each nuclide. Finally the deposition maps were created for (134)Cs, (137)Cs, (131)I, (129m)Te and (110m)Ag. The radioactivity ratio of (134)Cs-(137)Cs was almost constant at 0.91 regardless of the locations of soil sampling. The radioactivity ratios of (131)I and (129m)Te-(137)Cs were relatively high in the regions south of the Fukushima NPP site. Effective doses for 50 y after the accident were evaluated for external and inhalation exposures due to the observed radioactive nuclides. The radiation doses from radioactive cesium were found to be much higher than those from the other radioactive nuclides. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  14. Identification of hormone-interacting amino acid residues within the steroid-binding domain of the glucocorticoid receptor in relation to other steroid hormone receptors

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Carlstedt-Duke, J.; Stroemstedt, P.E.; Persson, B.

    1988-05-15

    Purified rat liver glucocorticoid receptor was covalently charged with (/sup 3/H)glucocorticoid by photoaffinity labeling (UV irradiation of (/sup 3/H)triamcinolone acetonide-glucocorticoid receptor) or affinity labeling (incubation with (/sup 3/H)dexamethasone mesylate). After labeling, separate samples of the denatured receptor were cleaved with trypsin (directly or after prior succinylation), chymotrypsin, and cyanogen bromide. Labeled residues in the peptides obtained were identified by radiosequence analysis. The peaks of radioactivity corresponded to Met-622 and Cys-754 after photoaffinity labeling with (/sup 3/H)triamcinolone acetonide and Cys-656 after affinity labeling with (/sup 3/H)dexamethasone mesylate. The labeled residues are all positioned within hydrophobic segments of the steroid-binding domain. Themore » patterns of hydropathy and secondary structure for the glucocorticoid receptor are highly similar to those for the progestin receptor and similar but less so to those for the estrogen receptor and to those for c-erb A.« less

  15. Halogenated naphthyl methoxy piperidines for mapping serotonin transporter sites

    DOEpatents

    Goodman, Mark M.; Faraj, Bahjat

    1999-01-01

    Halogenated naphthyl methoxy piperidines having a strong affinity for the serotonin transporter are disclosed. Those compounds can be labeled with positron-emitting and/or gamma emitting halogen isotopes by a late step synthesis that maximizes the useable lifeterm of the label. The labeled compounds are useful for localizing serotonin transporter sites by positron emission tomography and/or single photon emission computed tomography.

  16. Halogenated naphthyl methoxy piperidines for mapping serotonin transporter sites

    DOEpatents

    Goodman, M.M.; Faraj, B.

    1999-07-06

    Halogenated naphthyl methoxy piperidines having a strong affinity for the serotonin transporter are disclosed. Those compounds can be labeled with positron-emitting and/or gamma emitting halogen isotopes by a late step synthesis that maximizes the useable lifeterm of the label. The labeled compounds are useful for localizing serotonin transporter sites by positron emission tomography and/or single photon emission computed tomography.

  17. The potential for gamma-emitting radionuclides to contribute to an understanding of erosion processes in South Africa

    NASA Astrophysics Data System (ADS)

    Foster, Ian D. L.; Boardman, John; Collins, Adrian L.; Copeland-Phillips, Ruth; Kuhn, Nikolaus J.; Mighall, Tim M.; Pulley, Simon; Rowntree, Kate M.

    2017-03-01

    Several research projects undertaken by the authors and others over the last 14 years have used fallout and geogenic radionuclides for understanding erosion processes and sediment yield dynamics in South Africa over the last 100-200 years as European settlers colonised the interior plains and plateaux of the country and imported new livestock and farming techniques to the region. These projects have used two fallout radionuclides (210Pb and 137Cs) to date sediments accumulating in reservoirs, farm dams, wetlands, alluvial fans and floodouts and have used other fallout nuclides (7Be) and long-lived geogenic radionuclides (e.g. 40K, 235U) as part of a composite fingerprint exploring contemporary sediment sources and changes to sources through time. While successful in many parts of the world, applying these techniques in Southern Africa has posed a number of challenges often not encountered elsewhere. Here we explore some of the benefits and challenges in using gamma-emitting radionuclides, especially 137Cs, in these landscapes. Benefits include the potential for discriminating gully sidewall from topsoil sources, which has helped to identify contemporary gully systems as sediment conduits, rather than sources, and for providing a time-synchronous marker horizon in a range of sedimentary environments that has helped to develop robust chronologies. Challenges include the spatial variability in soil cover on steep rocky hillslopes, which is likely to challenge assumptions about the uniformity of initial fallout nuclide distribution, the paucity of stable (non-eroding) sites in order to estimate atmospheric fallout inventories, and the limited success of 210Pb dating in some rapidly accumulating high altitude catchments where sediments often comprise significant amounts of sand and gravel. Despite these challenges we present evidence suggesting that the use of gamma-emitting radionuclides can make a significant contribution to our understanding of erosion processes and

  18. Lymphocyte receptors for pertussis toxin

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Clark, C.G.; Armstrong, G.D.

    1990-12-01

    We have investigated human T-lymphocyte receptors for pertussis toxin by affinity isolation and photoaffinity labeling procedures. T lymphocytes were obtained from peripheral human blood, surface iodinated, and solubilized in Triton X-100. The iodinated mixture was then passed through pertussis toxin-agarose, and the fractions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Autoradiography of the fixed, dried gels revealed several bands in the pertussis toxin-bound fraction that were not observed in fractions obtained from histone or fetuin-agarose. Further investigations employed a photoaffinity labeling reagent, sulfosuccinimidyl 2-(p-azido-salicylamido)-1,3'-dithiopropionate, to identify pertussis toxin receptors in freshly isolated peripheral blood monocytic cells, T lymphocytes, andmore » Jurkat cells. In all three cell systems, the pertussis toxin affinity probe specifically labeled a single protein species with an apparent molecular weight of 70,000 that was not observed when the procedure was performed in the presence of excess unmodified pertussis toxin. A protein comparable in molecular weight to the one detected by the photoaffinity labeling technique was also observed among the species that bound to pertussis toxin-agarose. The results suggest that pertussis toxin may bind to a 70,000-Da receptor in human T lymphocytes.« less

  19. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kahoun, J.R.; Ruoho, A.E.

    A carrier-free radioiodinated cocaine photoaffinity label, (-)-3-({sup 125}I)iodo-4-azidococaine (({sup 125}I)IACoc), has been synthesized and used as a probe for cocaine-binding proteins. Photoaffinity labeling with 0.5 nM ({sup 125}I)IACoc resulted in selective derivatization of a 26-kDa polypeptide with the pharmacology of a sigma receptor in membranes derived from whole rat brain, rat liver, and human placenta. ({sup 125}I)IACoc labeling of the 26-kDa polypeptide was also inhibited by 10 {mu}M imipramine, amitriptyline, fluoxetine, benztropine, and tetrabenazine. The size of the ({sup 125}I)I-ACoc-labeled proteins is consistent with the size of proteins photolabeled in guinea pig brain and liver membranes by using the sigmamore » photolabel azido-({sup 3}H)DTG. Kinetic analysis of ({sup 125}I)IACoc binding to rat liver microsomes revealed two sites with K{sub d} values of 19 and 126 pM, respectively. The presence or absence of proteolytic inhibitors during membrane preparation did not alter the size of the photolabeled sigma receptor, indicating that the 26-kDa polypeptide was not derived from a larger protein. In summary, ({sup 125}I)IACoc is a potent and highly specific photoaffinity label for the haloperidol-sensitive sigma receptor and will be useful for its biochemical and molecular characterization.« less

  20. ASTRONOMY: Neighborhood Gamma Ray Burst Boosts Theory.

    PubMed

    Schilling, G

    2000-07-07

    Titanic explosions that emit powerful flashes of energetic gamma rays are one of astronomy's hottest mysteries. Now an analysis of the nearest gamma ray burst yet detected has added weight to the popular theory that they are expelled during the death throes of supermassive stars.

  1. Photoaffinity labeling of protoporphyrinogen oxidase, the molecular target of diphenylether-type herbicides.

    PubMed

    Camadro, J M; Matringe, M; Thome, F; Brouillet, N; Mornet, R; Labbe, P

    1995-05-01

    Diphenylether-type herbicides are extremely potent inhibitors of protoporphyrinogen oxidase, a membrane-bound enzyme involved in the heme and chlorophyll biosynthesis pathways. Tritiated acifluorfen and a diazoketone derivative of tritiated acifluorfen were specifically bound to a single class of high-affinity binding sites on yeast mitochondrial membranes with apparent dissociation constants of 7 nM and 12.5 nM, respectively. The maximum density of specific binding sites, determined by Scatchard analysis, was 3 pmol.mg-1 protein. Protoporphyrinogen oxidase specific activity was estimated to be 2500 nmol protoporphyrinogen oxidized h-1.mol-1 enzyme. The diazoketone derivative of tritiated acifluorfen was used to specifically photolabel yeast protoporphyrinogen oxidase. The specifically labeled polypeptide in wild-type mitochondrial membranes had an apparent molecular mass of 55 kDa, identical to the molecular mass of the purified enzyme. This photolabeled polypeptide was not detected in a protoporphyrinogen-oxidase-deficient yeast strain, but the membranes contained an equivalent amount of inactive immunoreactive protoporphyrinogen oxidase protein.

  2. Photoaffinity labeling of the primer binding domain in murine leukemia virus reverse transcriptase.

    PubMed

    Tirumalai, R S; Modak, M J

    1991-07-02

    We have labeled the primer binding domain of murine leukemia virus reverse transcriptase (MuLV RT) by covalently cross-linking 5' end labeled d(T)8 to MuLV RT, using ultraviolet light energy. The specificity and the functional significance of the primer cross-linking reaction were demonstrated by the fact that (i) other oligomeric primers, tRNAs, and also template-primers readily compete with radiolabeled d(T)8 for the cross-linking reaction, (ii) under similar conditions, the competing primers and template-primer also inhibit the DNA polymerase activity of MuLV RT to a similar extent, (iii) substrate deoxynucleotides have no effect, and (iv) the reaction is sensitive to high ionic strength. In order to identify the primer binding domains/sites in MuLV RT; tryptic digests prepared from the covalently cross-linked MuLV RT and [32P]d(T)8 complexes were resolved on C-18 columns by reverse-phase HPLC. Three distinct radiolabeled peptides were found to contain the majority of the bound primer. Of these, peptide I contained approximately 65% radioactivity, while the remainder was associated with peptides II and III. Amino acid composition and sequence analyses of the individual peptides revealed that peptide I spans amino acid residues 72-80 in the primary amino acid sequence of MuLV RT and is located in the polymerase domain. The primer cross-linking site appears to be at or near Pro-76. Peptides II and III span amino acid residues 602-609 and 615-622, respectively, and are located in the RNase H domain. The probable cross-linking sites in peptides II and III are suggested to be at or near Leu-604 and Leu-618, respectively.

  3. Production and Applications of Long-Lived Positron-Emitting Radionuclides

    NASA Astrophysics Data System (ADS)

    Graves, Stephen A.

    Positron emission tomography (PET) is a medical imaging modality capable of determining the in vivo spatial distribution of a biologically relevant molecule which has been labeled with a positron-emitting isotope. The use of molecules such as monoclonal antibodies and nanoparticles for therapeutic and diagnostic applications has expanded preclinically in recent years. As these larger molecules tend to have longer circulation times and slow clearance kinetics, positron-emitting isotopes with half-lives longer than conventional medical radioisotopes are required for PET applications. This dissertation details methods for the production of 51Mn (t1/2: 45.4 min), 52gMn (t1/2: 5.59 d), 64Cu (t1/2: 12.7 h), 76Br (t1/2: 16.2 h), 89Zr (t1/2: 3.27 d), and 194Au (t1/2: 38.0 h) on low-energy medical cyclotrons, including targetry considerations, radiochemical separation methods, and analysis of resulting purity. Pursuant to the production of these isotopes, several instrumentation developments have been made including implementation of an automatic nuclide identification library for gamma spectroscopy; development of methods for dead time correction and background estimation in auto-gamma counting; and the creation of a new linearly-filled Derenzo-type PET phantom. Measurement of the radioactive half-lives of 51Mn and 52gMn are presented in addition to their use in a variety of preclinical molecular imaging applications, including immunoPET, stem cell tracking, functional beta-cell mass determination, and probing the impact of isoflurane on acute pancreatic function. An analytic model of effective specific activity is formed and tested against preliminary trace metal analysis results. Measurements of excitation functions for the large-scale production of medically relevant isotopes, including 52gMn, at the Los Alamos National Laboratory Isotope Production Facility (100 MeV p+) are presented. The results described herein have enabled and informed a variety of novel

  4. Fragranced consumer products: Chemicals emitted, ingredients unlisted

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Steinemann, Anne C., E-mail: acstein@u.washington.ed; MacGregor, Ian C.; Gordon, Sydney M.

    2011-04-15

    Fragranced consumer products are pervasive in society. Relatively little is known about the composition of these products, due to lack of prior study, complexity of formulations, and limitations and protections on ingredient disclosure in the U.S. We investigated volatile organic compounds (VOCs) emitted from 25 common fragranced consumer products-laundry products, personal care products, cleaning supplies, and air fresheners-using headspace analysis with gas chromatography/mass spectrometry (GC/MS). Our analysis found 133 different VOCs emitted from the 25 products, with an average of 17 VOCs per product. Of these 133 VOCs, 24 are classified as toxic or hazardous under U.S. federal laws, andmore » each product emitted at least one of these compounds. For 'green' products, emissions of these compounds were not significantly different from the other products. Of all VOCs identified across the products, only 1 was listed on any product label, and only 2 were listed on any material safety data sheet (MSDS). While virtually none of the chemicals identified were listed, this nonetheless accords with U.S. regulations, which do not require disclosure of all ingredients in a consumer product, or of any ingredients in a mixture called 'fragrance.' Because the analysis focused on compounds emitted and listed, rather than exposures and effects, it makes no claims regarding possible risks from product use. Results of this study contribute to understanding emissions from common products, and their links with labeling and legislation.« less

  5. Near-infrared emitting fluorescent nanocrystals-labeled natural killer cells as a platform technology for the optical imaging of immunotherapeutic cells-based cancer therapy

    NASA Astrophysics Data System (ADS)

    Taik Lim, Yong; Cho, Mi Young; Noh, Young-Woock; Chung, Jin Woong; Chung, Bong Hyun

    2009-11-01

    This study describes the development of near-infrared optical imaging technology for the monitoring of immunotherapeutic cell-based cancer therapy using natural killer (NK) cells labeled with fluorescent nanocrystals. Although NK cell-based immunotherapeutic strategies have drawn interest as potent preclinical or clinical methods of cancer therapy, there are few reports documenting the molecular imaging of NK cell-based cancer therapy, primarily due to the difficulty of labeling of NK cells with imaging probes. Human natural killer cells (NK92MI) were labeled with anti-human CD56 antibody-coated quantum dots (QD705) for fluorescence imaging. FACS analysis showed that the NK92MI cells labeled with anti-human CD56 antibody-coated QD705 have no effect on the cell viability. The effect of anti-human CD56 antibody-coated QD705 labeling on the NK92MI cell function was investigated by measuring interferon gamma (IFN- γ) production and cytolytic activity. Finally, the NK92MI cells labeled with anti-human CD56 antibody-coated QD705 showed a therapeutic effect similar to that of unlabeled NK92MI cells. Images of intratumorally injected NK92MI cells labeled with anti-human CD56 antibody-coated could be acquired using near-infrared optical imaging both in vivo and in vitro. This result demonstrates that the immunotherapeutic cells labeled with fluorescent nanocrystals can be a versatile platform for the effective tracking of injected therapeutic cells using optical imaging technology, which is very important in cell-based cancer therapies.

  6. Assessing antibody microarrays for space missions: effect of long-term storage, gamma radiation, and temperature shifts on printed and fluorescently labeled antibodies.

    PubMed

    de Diego-Castilla, Graciela; Cruz-Gil, Patricia; Mateo-Martí, Eva; Fernández-Calvo, Patricia; Rivas, Luis A; Parro, Víctor

    2011-10-01

    Antibody microarrays are becoming frequently used tools for analytical purposes. A key factor for optimal performance is the stability of the immobilized (capturing) antibodies as well as those that have been fluorescently labeled to achieve the immunological test (tracers). This is especially critical for long-distance transport, field testing, or planetary exploration. A number of different environmental stresses may affect the antibody integrity, such as dryness, sudden temperature shift cycles, or, as in the case of space science, exposure to large quantities of the highly penetrating gamma radiation. Here, we report on the effect of certain stabilizing solutions for long-term storage of printed antibody microarrays under different conditions. We tested the effect of gamma radiation on printed and freeze- or vacuum-dried fluorescent antibodies at working concentrations (tracer antibodies), as well as the effect of multiple cycles of sudden and prolonged temperature shifts on the stability of fluorescently labeled tracer antibody cocktails. Our results show that (i) antibody microarrays are stable at room temperature when printed on stabilizing spotting solutions for at least 6 months, (ii) lyophilized and vacuum-dried fluorescently labeled tracer antibodies are stable for more than 9 months of sudden temperature shift cycles (-20°C to 25°C and 50°C), and (iii) both printed and freeze- or vacuum-dried fluorescent tracer antibodies are stable after several-fold excess of the dose of gamma radiation expected during a mission to Mars. Although different antibodies may exhibit different susceptibilities, we conclude that, in general, antibodies are suitable for use in planetary exploration purposes if they are properly treated and stored with the use of stabilizing substances.

  7. Implications of Gamma-Ray Transparency Constraints in Blazars: Minimum Distances and Gamma-Ray Collimation

    NASA Technical Reports Server (NTRS)

    Becker, Peter A.; Kafatos, Menas

    1995-01-01

    We develop a general expression for the gamma - gamma absorption coefficient, alpha(sub gamma(gamma)) for gamma-rays propagating in an arbitrary direction at an arbitrary point in space above an X-ray-emitting accretion disk. The X-ray intensity is assumed to vary as a power law in energy and radius between the outer disk radius, R(sub 0), and the inner radius, R(sub ms) which is the radius of marginal stability for a Schwarzschild black hole. We use our result for alpha(sub gamma(gamma)) to calculate the gamma - gamma optical depth, tau(sub gamma(gamma)) for gamma - rays created at height z and propagating at angle Phi relative to the disk axis, and we show that for Phi = 0 and z greater than or approx equal to R(sub 0), tau(sub gamma(gamma)) proportional to Epsilon(sup alpha)z(sup -2(alpha) - 3), where alpha is the X-ray spectral index and Epsilon is the gamma - ray energy. As an application, we use our formalism to compute the minimum distance between the central black hole and the site of production of the gamma-rays detected by EGRET during the 1991 June flare of 3C 279. In order to obtain an upper limit, we assume that all of the X-rays observed contemporaneously by Ginga were emitted by the disk. Our results suggest that the observed gamma - rays may have originated within less than or approx equal to 45 GM/sq c from a black hole of mass greater than or approx equal to 10(exp 9) solar mass, perhaps in active plasma located above the central funnel of the accretion disk. This raises the possibility of establishing a direct connection between the production of the observed gamma - rays and the accretion of material onto the black hole. We also consider the variation of the optical depth as a function of the angle of propagation Phi. Our results indicate that the "focusing" of the gamma - rays along the disk axis due to pair production is strong enough to explain the observed degree of alignment in blazar sources. If the gamma - rays are produced isotropically

  8. A NuSTAR Observation of the Gamma-Ray Emitting Millisecond Pulsar PSR J1723–2837

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kong, A. K. H.; Hui, C. Y.; Takata, J.

    We report on the first NuSTAR observation of the gamma-ray emitting millisecond pulsar binary PSR J1723–2837. X-ray radiation up to 79 keV is clearly detected, and the simultaneous NuSTAR and Swift spectrum is well described by an absorbed power law with a photon index of ∼1.3. We also find X-ray modulations in the 3–10, 10–20, 20–79, and 3–79 keV bands at the 14.8 hr binary orbital period. All of these are entirely consistent with previous X-ray observations below 10 keV. This new hard X-ray observation of PSR J1723–2837 provides strong evidence that the X-rays are from the intrabinary shock viamore » an interaction between the pulsar wind and the outflow from the companion star. We discuss how the NuSTAR observation constrains the physical parameters of the intrabinary shock model.« less

  9. Selective Photoaffinity Labeling Identifies the Signal Peptide Binding Domain on SecA

    PubMed Central

    Musial-Siwek, Monika; Rusch, Sharyn L.; Kendall, Debra A.

    2007-01-01

    SecA, an ATPase crucial to the Sec-dependent translocation machinery in Escherichia coli, recognizes and directly binds the N-terminal signal peptide of an exported preprotein. This interaction plays a central role in the targeting and transport of preproteins via the SecYEG channel. Here we identify the Signal Peptide Binding Groove (SPBG) on SecA addressing a key issue regarding the SecA-preprotein interaction. We employ a synthetic signal peptide containing the photoreactive benzoylphenylalanine to efficiently and specifically label SecA containing a unique Factor Xa site. Comparison of the photolabeled fragment from the subsequent proteolysis of several SecAs, which vary only in the location of the Factor Xa site, reveals one 53-residue segment in common with the entire series. The covalently modified SecA segment produced is the same in aqueous solution and in lipid vesicles. This spans amino acids 269 to 322 of the E. coli protein, which is distinct from a previously proposed signal peptide binding site, and contributes to a hydrophobic peptide binding groove evident in molecular models of SecA. PMID:17084862

  10. Using mass spectrometry to study the photo-affinity labeling of protein tyrosine phosphatase 1B

    NASA Astrophysics Data System (ADS)

    Leriche, Tammy; Skorey, Kathryn; Roy, Patrick; McKay, Dan; Bateman, Kevin P.

    2004-11-01

    Protein tyrosine phosphatase 1B (PTP1B) is a potential target for the treatment of Type II diabetes and several companies are developing small molecule inhibitors of this enzyme. Part of the characterization of these compounds as PTP1B inhibitors is the understanding of how they bind in the enzyme active site. The use of photo-activated inhibitors that target the active site can provide such insight. This paper describes the characterization of a photoprobe directed at the active site of PTP1B. Mass spectrometry revealed the specific binding of the probe to the intact protein. Digestion of the labeled protein followed by LC-MS and LC-MS/MS was used to show that the photoprobe binds to a specific active site amino acid. This was confirmed by comparison with the X-ray structure of PTP1B with a PTP1B inhibitor. The probe labels a conserved acidic residue (Asp) that is required for catalytic activity. This photoprobe may prove to be a useful tool for the development of a PTP1B inhibitor or for the study of PTPs in general.

  11. Development of a three-layer phoswich alpha-beta-gamma imaging detector

    NASA Astrophysics Data System (ADS)

    Yamamoto, Seiichi; Ishibashi, Hiroyuki

    2015-06-01

    For radiation monitoring at the sites of such nuclear power plant accidents as Fukushima Daiichi, radiation detectors are needed not only for gamma photons but also for alpha and beta particles because some nuclear fission products emit beta particles and gamma photons and some nuclear fuels contain plutonium that emits alpha particles. In some applications, imaging detectors are required to detect the distribution of plutonium particles that emit alpha particles and radiocesium in foods that emits beta particles and gamma photons. To solve these requirements, we developed an imaging detector that can measure the distribution of alpha and beta particles as well as gamma photons. The imaging detector consists of three-layer scintillators optically coupled to each other and to a position sensitive photomultiplier tube (PSPMT). The first layer, which is made of a thin plastic scintillator (decay time: 5 ns), detects alpha particles. The second layer, which is made of a thin Gd2SiO5 (GSO) scintillator with 1.5 mol% Ce (decay time: 35 ns), detects beta particles. The third layer made of a thin GSO scintillator with 0.4 mol% Ce (decay time: 70 ns) detects gamma photons. Using pulse shape discrimination, the images of these layers can be separated. The position information is calculated by the Anger principle from 8×8 anode signals from the PSPMT. The images for the alpha and beta particles and the gamma photons are individually formed by the pulse shape discriminations for each layer. We detected alpha particle images in the first layer and beta particle images in the second layer. Gamma photon images were detected in the second and third layers. The spatial resolution for the alpha and beta particles was 1.25 mm FWHM and less than 2 mm FWHM for the gamma photons. We conclude that our developed alpha-beta-gamma imaging detector is promising for imaging applications not only for the environmental monitoring of radionuclides but also for medical and molecular imaging.

  12. Radionuclide labeled lymphocytes for therapeutic use

    DOEpatents

    Srivastava, Suresh C.; Fawwaz, Rashid A.; Richards, Powell

    1985-01-01

    Lymphocytes labelled with .beta.-emitting radionuclides are therapeutically useful, particularly for lymphoid ablation. They are prepared by incubation of the lymphocytes with the selected radionuclide-oxine complex.

  13. Radionuclide labeled lymphocytes for therapeutic use

    DOEpatents

    Srivastava, S.C.; Fawwaz, R.A.; Richards, P.

    1983-05-03

    Lymphocytes labelled with ..beta..-emitting radionuclides are therapeutically useful, particularly for lymphoid ablation. They are prepared by incubation of the lymphocytes with the selected radionuclide-oxine complex.

  14. Gamma ray spectroscopy in astrophysics: Solar gamma ray astronomy on solar maximum mission. [experimental design

    NASA Technical Reports Server (NTRS)

    Forrest, D. J.

    1978-01-01

    The SMM gamma ray experiment and the important scientific capabilities of the instrument are discussed. The flare size detectable as a function of spectrum integration time was studied. A preliminary estimate indicates that a solar gamma ray line at 4.4 MeV one-fifth the intensity of that believed to have been emitted on 4 August 1972 can be detected in approximately 1000 sec with a confidence level of 99%.

  15. Gamma radiation field intensity meter

    DOEpatents

    Thacker, Louis H.

    1994-01-01

    A gamma radiation intensity meter measures dose rate of a radiation field. The gamma radiation intensity meter includes a tritium battery emitting beta rays generating a current which is essentially constant. Dose rate is correlated to an amount of movement of an electroscope element charged by the tritium battery. Ionizing radiation decreases the voltage at the element and causes movement. A bleed resistor is coupled between the electroscope support element or electrode and the ionization chamber wall electrode.

  16. Gamma radiation field intensity meter

    DOEpatents

    Thacker, Louis H.

    1995-01-01

    A gamma radiation intensity meter measures dose rate of a radiation field. The gamma radiation intensity meter includes a tritium battery emitting beta rays generating a current which is essentially constant. Dose rate is correlated to an amount of movement of an electroscope element charged by the tritium battery. Ionizing radiation decreases the voltage at the element and causes movement. A bleed resistor is coupled between the electroscope support element or electrode and the ionization chamber wall electrode.

  17. Initial Gamma Spectrometry Examination of the AGR-3/4 Irradiation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Harp, Jason M.; Demkowicz, Paul A.; Stempien, John D.

    2016-11-01

    The initial results from gamma spectrometry examination of the different components from the combined third and fourth US Advanced Gas Reactor Fuel Development TRISO-coated particle fuel irradiation tests (AGR-3/4) have been analyzed. This experiment was designed to provide information about in-pile fission product migration. In each of the 12 capsules, a single stack of four compacts with designed-to-fail particles surrounded by two graphitic diffusion rings (inner and outer) and a graphite sink were irradiated in the Idaho National Laboratory’s Advanced Test Reactor. Gamma spectrometry has been used to evaluate the gamma-emitting fission product inventory of compacts from the irradiation andmore » evaluate the burnup of these compacts based on the activity of the radioactive cesium isotopes (Cs-134 and Cs-137) in the compacts. Burnup from gamma spectrometry compares well with predicted burnup from simulations. Additionally, inner and outer rings were also examined by gamma spectrometry both to evaluate the fission product inventory and the distribution of gamma-emitting fission products within the rings using gamma emission computed tomography. The cesium inventory of the scanned rings compares acceptably well with the expected inventory from fission product transport modeling. The inventory of the graphite fission product sinks is also being evaluated by gamma spectrometry.« less

  18. Distance Magic-Type and Distance Antimagic-Type Labelings of Graphs

    NASA Astrophysics Data System (ADS)

    Freyberg, Bryan J.

    Generally speaking, a distance magic-type labeling of a graph G of order n is a bijection l from the vertex set of the graph to the first n natural numbers or to the elements of a group of order n, with the property that the weight of each vertex is the same. The weight of a vertex x is defined as the sum (or appropriate group operation) of all the labels of vertices adjacent to x. If instead we require that all weights differ, then we refer to the labeling as a distance antimagic-type labeling. This idea can be generalized for directed graphs; the weight will take into consideration the direction of the arcs. In this manuscript, we provide new results for d-handicap labeling, a distance antimagic-type labeling, and introduce a new distance magic-type labeling called orientable Gamma-distance magic labeling. A d-handicap distance antimagic labeling (or just d-handicap labeling for short) of a graph G = ( V,E) of order n is a bijection l from V to the set {1,2,...,n} with induced weight function [special characters omitted]. such that l(xi) = i and the sequence of weights w(x 1),w(x2),...,w (xn) forms an arithmetic sequence with constant difference d at least 1. If a graph G admits a d-handicap labeling, we say G is a d-handicap graph. A d-handicap incomplete tournament, H(n,k,d ) is an incomplete tournament of n teams ranked with the first n natural numbers such that each team plays exactly k games and the strength of schedule of the ith ranked team is d more than the i + 1st ranked team. That is, strength of schedule increases arithmetically with strength of team. Constructing an H(n,k,d) is equivalent to finding a d-handicap labeling of a k-regular graph of order n.. In Chapter 2 we provide general constructions for every d for large classes of both n and k, providing breadfth and depth to the catalog of known H(n,k,d)'s. In Chapters 3 - 6, we introduce a new type of labeling called orientable Gamma-distance magic labeling. Let Gamma be an abelian group of order

  19. [The clinical importance of physiopathological studies of the bile salts performed using the gamma-emitting bile acid SeHCAT].

    PubMed

    Ferraris, R; Fracchia, M; Galatola, G

    1992-01-01

    The availability of the gamma-labelled bile acid 75SeHCAT, that allows a non-invasive assessment of the enterohepatic circulation of bile acids, has prompted in the last 10 years the implementation of several studies involving wide series of normal subjects and patients with various organic and functional bowel disorders. The clinical indications for performing a SeHCAT test have been clearly defined: the test can identify with high accuracy, in the setting of the irritable bowel syndrome, the patients with bile acid malabsorption that can be confidently and successfully treated with cholestyramine; it can also assess whether, and to what extent, the diarrhoea presenting in patients with intestinal organic disorders is due to bile acid malabsorption, permitting an optimal therapeutic strategy to be designed. The parameters of the hepatic handling of SeHCAT after bolus intravenous administration have been characterized in normals, and studies on various chronic hepatic disorders are now in progress. Interesting results are emerging from studies performed in patients with chronic non-obstructive cholestatic disease, where a specific defect in the excretion rate of SeHCAT is present: these studies may cast more light on the abnormalities of bile secretion and on the mechanism of action of drugs used to treat this condition, forming the rationale for the use of intravenous SeHCAT for hepatobiliary dynamic scintigraphy as a sophisticated liver function test. In conclusion, the SeHCAT test has become an important diagnostic tool for the gastroenterologist studying the diarrhoea, and awaits more studies to be used also by the hepatologist. The relatively long physical half-life of 75Se (180 days), preventing a wider use of the test, could theoretically be overcome by the synthesis of a similar gamma-labelled bile acid with a shorter half-life.

  20. Gamma watermarking

    DOEpatents

    Ishikawa, Muriel Y.; Wood, Lowell L.; Lougheed, Ronald W.; Moody, Kenton J.; Wang, Tzu-Fang

    2004-05-25

    A covert, gamma-ray "signature" is used as a "watermark" for property identification. This new watermarking technology is based on a unique steganographic or "hidden writing" digital signature, implemented in tiny quantities of gamma-ray-emitting radioisotopic material combinations, generally covertly emplaced on or within an object. This digital signature may be readily recovered at distant future times, by placing a sensitive, high energy-resolution gamma-ray detecting instrument reasonably precisely over the location of the watermark, which location may be known only to the object's owner; however, the signature is concealed from all ordinary detection means because its exceedingly low level of activity is obscured by the natural radiation background (including the gamma radiation naturally emanating from the object itself, from cosmic radiation and material surroundings, from human bodies, etc.). The "watermark" is used in object-tagging for establishing object identity, history or ownership. It thus may serve as an aid to law enforcement officials in identifying stolen property and prosecuting theft thereof. Highly effective, potentially very low cost identification-on demand of items of most all types is thus made possible.

  1. Synthesis and biological studies of positron-emitting radiopharmaceuticals

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dischino, D.D.

    The development and clinical evaluation of two-positron emitting radiopharmaceuticals designed to image myelin in humans is reported. Carbon-11-labeled benzyl methyl ether was synthesized by the reaction of carbon-11-labeled methanol and benzyl chloride in dimethyl sulfoxide containing powdered potassium hydroxide in a radiochemical yield of 43% and a synthesis and purification time of 40 minutes. Carbon-11-labeled diphenylmethanol was synthesized by the reaction of carbon-11-labeled carbon dioxide and phenyllithium followed by the reduction of the carbon-11-labeled intermediate to diphenylmethanol via lithium aluminum hydride in a radiochemical yield of 71% and a synthesis and purification time of 38 minutes. Carbon-11-labeled benzyl methyl ethermore » and diphenylmethanol were each evaluated as myelin imaging agents in three patients with multiple sclerosis via positron-emission tomography. In two out of three patients studied with carbon-11-labeled benzyl methyl ether, the distribution of activity in the brain was not consistent with local lipid content. A new synthesis of carbon-11-labeled-DL-phenylalanine labeled in the benzylic position and the synthesis of fluorine-18-labeled 1-(2-nitro-1-imidazolyl)-3-fluoro-2-propanol, a potential in vivo marker of hypoxic tissue, are reported.« less

  2. Method for on-line evaluation of materials using prompt gamma ray analysis

    DOEpatents

    Akers, Douglas W [Idaho Falls, ID

    2009-12-08

    A method for evaluating a material specimen comprises: Mounting a neutron source and a detector adjacent the material specimen; bombarding the material specimen with neutrons from the neutron source to create prompt gamma rays within the material specimen, some of the prompt gamma rays being emitted from the material specimen, some of the prompt gamma rays resulting in the formation of positrons within the material specimen by pair production; collecting positron annihilation data by detecting with the detector at least one emitted annihilation gamma ray resulting from the annihilation of a positron; storing the positron annihilation data on a data storage system for later retrieval and processing; and continuing to collect and store positron annihilation data, the continued collected and stored positron annihilation data being indicative of an accumulation of lattice damage over time.

  3. Gamma radiation field intensity meter

    DOEpatents

    Thacker, L.H.

    1995-10-17

    A gamma radiation intensity meter measures dose rate of a radiation field. The gamma radiation intensity meter includes a tritium battery emitting beta rays generating a current which is essentially constant. Dose rate is correlated to an amount of movement of an electroscope element charged by the tritium battery. Ionizing radiation decreases the voltage at the element and causes movement. A bleed resistor is coupled between the electroscope support element or electrode and the ionization chamber wall electrode. 4 figs.

  4. Gamma radiation field intensity meter

    DOEpatents

    Thacker, L.H.

    1994-08-16

    A gamma radiation intensity meter measures dose rate of a radiation field. The gamma radiation intensity meter includes a tritium battery emitting beta rays generating a current which is essentially constant. Dose rate is correlated to an amount of movement of an electroscope element charged by the tritium battery. Ionizing radiation decreases the voltage at the element and causes movement. A bleed resistor is coupled between the electroscope support element or electrode and the ionization chamber wall electrode. 4 figs.

  5. Fluorescence-Assisted Gamma Spectrometry for Surface Contamination Analysis

    NASA Astrophysics Data System (ADS)

    Ihantola, Sakari; Sand, Johan; Perajarvi, Kari; Toivonen, Juha; Toivonen, Harri

    2013-02-01

    A fluorescence-based alpha-gamma coincidence spectrometry approach has been developed for the analysis of alpha-emitting radionuclides. The thermalization of alpha particles in air produces UV light, which in turn can be detected over long distances. The simultaneous detection of UV and gamma photons allows detailed gamma analyses of a single spot of interest even in highly active surroundings. Alpha particles can also be detected indirectly from samples inside sealed plastic bags, which minimizes the risk of cross-contamination. The position-sensitive alpha-UV-gamma coincidence technique reveals the presence of alpha emitters and identifies the nuclides ten times faster than conventional gamma spectrometry.

  6. A simultaneous beta and coincidence-gamma imaging system for plant leaves

    NASA Astrophysics Data System (ADS)

    Ranjbar, Homayoon; Wen, Jie; Mathews, Aswin J.; Komarov, Sergey; Wang, Qiang; Li, Ke; O'Sullivan, Joseph A.; Tai, Yuan-Chuan

    2016-05-01

    Positron emitting isotopes, such as 11C, 13N, and 18F, can be used to label molecules. The tracers, such as 11CO2, are delivered to plants to study their biological processes, particularly metabolism and photosynthesis, which may contribute to the development of plants that have a higher yield of crops and biomass. Measurements and resulting images from PET scanners are not quantitative in young plant structures or in plant leaves due to poor positron annihilation in thin objects. To address this problem we have designed, assembled, modeled, and tested a nuclear imaging system (simultaneous beta-gamma imager). The imager can simultaneously detect positrons ({β+} ) and coincidence-gamma rays (γ). The imaging system employs two planar detectors; one is a regular gamma detector which has a LYSO crystal array, and the other is a phoswich detector which has an additional BC-404 plastic scintillator for beta detection. A forward model for positrons is proposed along with a joint image reconstruction formulation to utilize the beta and coincidence-gamma measurements for estimating radioactivity distribution in plant leaves. The joint reconstruction algorithm first reconstructs beta and gamma images independently to estimate the thickness component of the beta forward model and afterward jointly estimates the radioactivity distribution in the object. We have validated the physics model and reconstruction framework through a phantom imaging study and imaging a tomato leaf that has absorbed 11CO2. The results demonstrate that the simultaneously acquired beta and coincidence-gamma data, combined with our proposed joint reconstruction algorithm, improved the quantitative accuracy of estimating radioactivity distribution in thin objects such as leaves. We used the structural similarity (SSIM) index for comparing the leaf images from the simultaneous beta-gamma imager with the ground truth image. The jointly reconstructed images yield SSIM indices of 0.69 and 0.63, whereas the

  7. A simultaneous beta and coincidence-gamma imaging system for plant leaves.

    PubMed

    Ranjbar, Homayoon; Wen, Jie; Mathews, Aswin J; Komarov, Sergey; Wang, Qiang; Li, Ke; O'Sullivan, Joseph A; Tai, Yuan-Chuan

    2016-05-07

    Positron emitting isotopes, such as (11)C, (13)N, and (18)F, can be used to label molecules. The tracers, such as (11)CO2, are delivered to plants to study their biological processes, particularly metabolism and photosynthesis, which may contribute to the development of plants that have a higher yield of crops and biomass. Measurements and resulting images from PET scanners are not quantitative in young plant structures or in plant leaves due to poor positron annihilation in thin objects. To address this problem we have designed, assembled, modeled, and tested a nuclear imaging system (simultaneous beta-gamma imager). The imager can simultaneously detect positrons ([Formula: see text]) and coincidence-gamma rays (γ). The imaging system employs two planar detectors; one is a regular gamma detector which has a LYSO crystal array, and the other is a phoswich detector which has an additional BC-404 plastic scintillator for beta detection. A forward model for positrons is proposed along with a joint image reconstruction formulation to utilize the beta and coincidence-gamma measurements for estimating radioactivity distribution in plant leaves. The joint reconstruction algorithm first reconstructs beta and gamma images independently to estimate the thickness component of the beta forward model and afterward jointly estimates the radioactivity distribution in the object. We have validated the physics model and reconstruction framework through a phantom imaging study and imaging a tomato leaf that has absorbed (11)CO2. The results demonstrate that the simultaneously acquired beta and coincidence-gamma data, combined with our proposed joint reconstruction algorithm, improved the quantitative accuracy of estimating radioactivity distribution in thin objects such as leaves. We used the structural similarity (SSIM) index for comparing the leaf images from the simultaneous beta-gamma imager with the ground truth image. The jointly reconstructed images yield SSIM indices of 0

  8. The Plasma Disappearance Time and Catabolic Half-Life of I 131-Labelled Normal Human Gamma Globulin in Amyloidosis and in Rheumatoid Arthritis

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mills, John A.; Calkins, Evan; Cohen, Alan S.

    1961-10-01

    The serum survival time and catabclic half-life of intravenously injected I 131-labeled pooled human gamma globulin - were studied in three patients with amyloidosis, four patients with rheumatoid arthritis, and three normal controls. The half-time of gamma globulin survival in the controsubjects ranged from 16.5 to 30 days. Two patients with amyloidosis, one primary and one secondary, both with the nephrotic syndrome, exhibited shortened serum half-times of 4.5 and 11 days, respectively. The serum half-time of the latter patient, before the appearance of clinical amyloidosis, was 14 days. One patient with primary amyloidosis but without nephrosis exhibited a half-time ofmore » serum gamma globulin disappearance of 21 days. The half-time of gamma globulin disappearance in four patients with chronic active rheumatoid arthritis varied between 19.5 and 8.5 days. The lower figure was found in a patient having a high titer of rheumatoid factor. If this subject is excepted, the average half- time in three rheumatoid subjects is 17 days. The catabolic half-life of the iodinated gamma globulin agreed in most instances with the serum half-time. The calculated distribution space of the injected gamma globulin showed no consistent alteration in either amyloidosis or rheumatoid arthritis as compared with the control subjects. Since the nephrotic syndrome from other causes may produce an accelerated catabolic half-life, a similar finding on these subjects cannot be ascribed to amyloidosis.« less

  9. Measurement of absolute gamma emission probabilities

    NASA Astrophysics Data System (ADS)

    Sumithrarachchi, Chandana S.; Rengan, Krish; Griffin, Henry C.

    2003-06-01

    The energies and emission probabilities (intensities) of gamma-rays emitted in radioactive decays of particular nuclides are the most important characteristics by which to quantify mixtures of radionuclides. Often, quantification is limited by uncertainties in measured intensities. A technique was developed to reduce these uncertainties. The method involves obtaining a pure sample of a nuclide using radiochemical techniques, and using appropriate fractions for beta and gamma measurements. The beta emission rates were measured using a liquid scintillation counter, and the gamma emission rates were measured with a high-purity germanium detector. Results were combined to obtain absolute gamma emission probabilities. All sources of uncertainties greater than 0.1% were examined. The method was tested with 38Cl and 88Rb.

  10. Qualitative variation of photolabelled benzodiazepine receptors in different species.

    PubMed

    Hebebrand, J; Friedl, W; Lentes, K U; Propping, P

    1986-01-01

    In order to examine whether species differences of benzodiazepine receptor subunits exist, we compared the fluorographic pattern of photoaffinity labelled subunits after SDS-PAGE in five species: fish, frog, chicken, mouse and calf. Each species showed a distinct pattern of specifically labelled proteins. We conclude that species variation of benzodiazepine receptor does indeed exist.

  11. Radiosequence analysis of the human progestin receptor charged with ( sup 3 H)promegestone. A comparison with the glucocorticoid receptor

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stroemstedt, P.E.B.; Berkenstam, A.; Joernvall, H.G.

    1990-08-05

    Partially purified preparations of the human progestin receptor and the human and rat glucocorticoid receptor proteins were covalently charged with the synthetic progestin, ({sup 3}H)promegestone, by photoaffinity labeling. After labeling, the denaturated protein was cleaved and the mixture of peptides subjected to radiosequence analysis as previously described for the rat glucocorticoid receptor protein. The radioactivity labels identified, corresponded to Met-759 and Met-909 after photoaffinity labeling of the human progestin receptor, and Met-622 and Cys-754 after labeling of the rat glucocorticoid receptor. The residues labeled in the glucocorticoid receptor are the same as those previously reported to bind triamcinolone actonide. Themore » corresponding residues were also labeled in the human glucocorticoid receptor. Met-759 of the progestin receptor and Met-622 of the rat glucocorticoid receptor are positioned within a segment with an overall high degree of sequence similarity and are equivalent. However, Met-909 (progestin receptor) and Cys-754 (glucocorticoid receptor) do not occur within equivalent segments of the two proteins. Thus, although the two classes of steroid hormone share a common structure within the A-ring, there are subtle differences in their interaction with the two separate receptor proteins.« less

  12. Synthesis of aryl azide derivatives of UDP-GlcNAc and UDP-GalNAc and their use for the affinity labeling of glycosyltransferases and the UDP-HexNAc pyrophosphorylase.

    PubMed

    Zeng, Y; Shabalin, Y; Szumilo, T; Pastuszak, I; Drake, R R; Elbein, A D

    1996-07-15

    The chemical synthesis and utilization of two photoaffinity analogs, 125I-labeled 5-[3-(p-azidosalicylamido)-1-propenyl]-UDP-GlcNAc and -UDP-GalNAc, is described. Starting with either UDP-GlcNAc or UDP-GalNAc, the synthesis involved the preparation of the 5-mercuri-UDP-HexNAc and then attachment of an allylamine to the 5 position to give 5-(3-amino)allyl-UDP-HexNAc. This was followed by acylation with N-hydroxysuccinimide p-aminosalicylic acid to form the final product, i.e., 5-[3-(p-azidosalicylamido)-1-propenyl]-UDP-GlcNAc or UDP-GalNAc. These products could then be iodinated with chloramine T to give the 125I-derivatives. Both the UDP-GlcNAc and the UDP-GalNAc derivatives reacted in a concentration-dependent manner with a highly purified UDP-HexNAc pyrophosphorylase, and both specifically labeled the subunit(s) of this protein. The labeling of the protein by the UDP-GlcNAc derivative was inhibited in dose-dependent fashion by either unlabeled UDP-GlcNAc or unlabeled UDP-GalNAc. Likewise, labeling with the UDP-GalNAc probe was blocked by either UDP-GlcNAc or UDP-GalNAc. The UDP-GlcNAc probe also specifically labeled a partially purified preparation of GlcNAc transferase I.

  13. DISCERNING THE GAMMA-RAY-EMITTING REGION IN THE FLAT SPECTRUM RADIO QUASARS

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zheng, Y. G.; Yang, C. Y.; Wang, J. C.

    2017-01-01

    A model-dependent method is proposed to determine the location of the γ -ray-emitting region for a given flat spectrum radio quasar (FSRQ). In the model, the extra-relativistic electrons are injected at the base of the jet and non-thermal photons are produced by both synchrotron radiation and inverse-Compton (IC) scattering in the energy dissipation region. The target photons dominating IC scattering originate from both synchrotron photons and external ambient photon fields, and the energy density of external radiation field is a function of the distance between the position of the dissipation region and a central supermassive black hole, and their spectramore » are seen in the comoving frame. Moreover, the energy dissipation region could be determined by the model parameter through reproducing the γ -ray spectra. Such a model is applied to reproduce the quasi-simultaneous multi-wavelength observed data for 36 FSRQs. In order to define the width of the broad-line region (BLR) shell and dusty molecular torus (MT) shell, a simple numerical constraint is used to determine the outer boundary of the BLR and dusty MT. Our results show that (1) the γ -ray-emitting regions are located at the range from 0.1 to 10 pc; (2) the γ -ray-emitting regions are located outside the BLRs and within the dusty molecular tori; and (3) the γ -ray-emitting regions are located closer to the dusty MT ranges than the BLRs. Therefore, it may be concluded that direct evidence for the far site scenario could be obtained on the basis of the model results.« less

  14. Gamma ray bursts: Current status of observations and theory

    NASA Technical Reports Server (NTRS)

    Meegan, Charles A.

    1990-01-01

    Gamma-ray bursts display a wide range of temporal and spectral characteristics, but typically last several seconds and emit most of their energy in the low-energy gamma-ray region. The burst sources appear to be isotropically distributed on the sky. Several lines of evidence suggest magnetic neutron stars as sources for bursts. A variety of energy sources and emission mechanisms were proposed.

  15. Gamma ray bursts: Current status of observations and theory

    NASA Technical Reports Server (NTRS)

    Meegan, Charles A.

    1990-01-01

    Gamma ray bursts display a wide range of temporal and spectral characteristics, but typically last several seconds and emit most of their energy in a low energy, gamma ray region. The burst sources appear to be isotropically distributed on the sky. Several lines of evidence suggest magnetic neutron stars as sources for bursts. A variety of energy sources and emission mechanisms are proposed.

  16. Gamma compensated, self powered neutron detector

    DOEpatents

    Brown, Donald P.

    1977-01-01

    An improved, self-powered, gamma compensated, neutron detector having two electrically conductive concentric cylindrical electrodes and a central rod emitter formed from a material which emits beta particles when bombarded by neutrons. The outer electrode and emitter are maintained at a common potential and the neutron representative current is furnished at the inner cylindrical electrode which serves as a collector. The two concentric cylindrical electrodes are designed to exhibit substantially equal electron emission induced by Compton scattering under neutron bombardment to supply the desired gamma compensation.

  17. DEATH LINE OF GAMMA-RAY PULSARS WITH OUTER GAPS

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wang, Ren-Bo; Hirotani, Kouichi, E-mail: rbwang1225@gmail.com, E-mail: hirotani@tiara.sinica.edu.tw

    2011-08-01

    We analytically investigate the condition for a particle accelerator to be active in the outer magnetosphere of a rotation-powered pulsar. Within the accelerator (or the gap), the magnetic-field-aligned electric field accelerates electrons and positrons, which emit copious gamma-rays via the curvature process. If one of the gamma-rays emitted by a single pair materializes as a new pair on average, the gap is self-sustained. However, if the neutron-star spin-down rate decreases below a certain limit, the gap becomes no longer self-sustained and the gamma-ray emission ceases. We explicitly compute the multiplicity of cascading pairs and find that the obtained limit correspondsmore » to a modification of the previously derived outer-gap death line. In addition to this traditional death line, we find another death line, which becomes important for millisecond pulsars, by separately considering the threshold of photon-photon pair production. Combining these traditional and new death lines, we give predictions on the detectability of gamma-ray pulsars with Fermi and AGILE. An implication for X-ray observations of heated polar-cap emission is also discussed.« less

  18. Photoactivable analogs for labeling 25-hydroxyvitamin D3 serum binding protein and for 1,25-dihydroxyvitamin D3 intestinal receptor protein

    NASA Technical Reports Server (NTRS)

    Kutner, A.; Link, R. P.; Schnoes, H. K.; DeLuca, H. F.

    1986-01-01

    3-Azidobenzoates and 3-azidonitrobenzoates of 25-hydroxyvitamin D3 as well as 3-deoxy-3-azido-25-hydroxyvitamin D3 and 3-deoxy-3-azido-1,25-dihydroxyvitamin D3 were prepared as photoaffinity labels for vitamin D serum binding protein and 1,25-dihydroxyvitamin D3 intestinal receptor protein. The compounds prepared were easily activated by short- or long-wavelength uv light, as monitored by uv and ir spectrometry. The efficacy of the compounds to compete with 25-hydroxyvitamin D3 or 1,25-dihydroxyvitamin D3 for the binding site of serum binding protein and receptor, respectively, was studied to evaluate the vitamin D label with the highest affinity for the protein. The presence of an azidobenzoate or azidonitrobenzoate substituent at the C-3 position of 25-OH-D3 significantly decreased (10(4)- to 10(6)-fold) the binding activity. However, the labels containing the azido substituent attached directly to the vitamin D skeleton at the C-3 position showed a high affinity, only 20- to 150-fold lower than that of the parent compounds with their respective proteins. Therefore, 3-deoxy-3-azidovitamins present potential ligands for photolabeling of vitamin D proteins and for studying the structures of the protein active sites.

  19. Bromine-80m-labeled estrogens: Auger-electron emitting, estrogen receptor-directed ligands with potential for therapy of estrogen receptor positive cancers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    DeSombre, E.R.; Mease, R.C.; Hughes, A.

    1988-01-01

    A triphenylbromoethylene, 1,1-bis(p-hydroxyphenyl)-2-bromo-2-phenylethylene, Br-BHPE, and a bromosteroidal estrogen, 17..cap alpha..- bromovinylestradiol, BrVE/sub 2/, were labeled with the Auger electron emitting nuclide bromine-80m, prepared by the (p,n) reaction with /sup 80/Se. To assess their potential as estrogen receptor (ER) directed therapeutic substrates the bromine-80m labeled estrogens were injected into immature female rats and the tissue distribution studied at 0.5 and 2 hours. Both radiobromoestrogens showed substantial diethylstilbesterol (DES)-inhibitable localization in the ER rich tissues, uterus, pituitary, ovary and vagina at both time points. While the percent dose per gram tissue was higher for the Br-BHPE, the BrVE/sub 2/ showed higher tissuemore » to blood ratios, especially at 2 hr, reflecting the lower blood concentrations of radiobromine following administration of the steroidal bromoestrogen. Comparing intraperitoneal, intravenous and subcutaneous routes of administration for the radiobromine labeled Br-BHPE, the intraperitoneal route was particularly advantageous to provide maximum, DES-inhibitable concentrations in the peritoneal, ER-rich target organs, the uterus, ovary and vagina. While uterine concentrations after BrBHPE were from 10--48% dose/g and after BrVE/sub 2/ were 15--25% dose/g, similar treatment with /sup 80m/Br as sodium bromide showed uniform low concentrations in all tissues at about the levels seen in blood. The effective specific activity of (/sup 80m/Br)BrBHPE, assayed by specific binding to ER in rat uterine cytosol, was 8700 Ci/mmole. 23 refs., 9 figs., 2 tabs.« less

  20. Development of a system for real-time measurements of metabolite transport in plants using short-lived positron-emitting radiotracers

    NASA Astrophysics Data System (ADS)

    Kiser, Matthew R.

    be introduced to plants as biologically active molecules such as 11CO2, N13O-3, 18F--[H2O], and H152O . Plants for these studies are grown in controlled-environment chambers at the Phytotron. The chambers offer an array of control for temperature, humidity, atmospheric CO2 concentration, and light intensity. Additionally, the Phytotron houses one large reach-in growth chamber that is dedicated to this project for radioisotope labeling measurements. There are several important properties of short-lived positron-emitting radio-tracers that make them well suited for use in investigating metabolite transport in plants. First, because the molecular mass of a radioisotope-tagged compound is only minutely different from the corresponding stable compound, radiotracer substances should be metabolized and transported in plants the same as their non-radioactive counterparts. Second, because the relatively high energy gamma rays emitted from electron-positron annihilation are attenuated very little by plant tissue, the real-time distribution of a radiotracer can be measured in vivo in plants. Finally, the short radioactive half-lives of these isotopes allow for repeat measurements on the same plant in a short period of time. For example, in studies of short-term environmental changes on plant metabolite dynamics, a single plant can be labeled multiple times to measure its responses to different, environmental conditions. Also, different short-lived radiotracers can be applied to the same plant over a short period of time to investigate the transport and allocation of various metabolites. This newly developed system provides the capabilities for production of 11CO2 at TUNL, transfer of the 11CO 2 gas from the target area at TUNL to a radiation-shielded cryogenic trap at the Phytotron, labeling of photoassimilates with 11C, and in vivo gamma-ray detection for real-time measurements of the radiotracer distribution in small plants. The experimental techniques and instrumentation

  1. Emitted radiation characteristics of plutonium dioxide radioisotope thermoelectric generators

    NASA Technical Reports Server (NTRS)

    Gingo, P. J.; Steyn, J. J.

    1971-01-01

    The nuclear and emitted radiation characteristics of the radioisotope elements and impurities in commercial grade plutonium dioxide are presented in detail. The development of the methods of analysis are presented. Radioisotope thermoelectric generators (RTG) of 1575, 3468 and 5679 thermal watts are characterized with respect to neutron and gamma photon source strength as well as spatial and number flux distribution. The results are presented as a function of detector position and light element contamination concentration for fuel age ranging from 'fresh' to 18 years. The data may be used to obtain results for given O-18 and Pu-236 concentrations. The neutron and gamma photon flux and dose calculations compare favorably with reported experimental values for SNAP-27.

  2. Palladium-109 labeled anti-melanoma monoclonal antibodies

    DOEpatents

    Srivastava, S.C.; Fawwaz, R.A.; Ferrone, S.

    1984-04-30

    The invention consists of new monoclonal antibodies labelled with Palladium 109, a beta-emitting radionuclide, the method of preparing this material, and its use in the radiotherapy of melanoma. The antibodies are chelate-conjugated and demonstrate a high uptake in melanomas. (ACR)

  3. Prompt gamma-ray emission from biological tissues during proton irradiation: a preliminary study.

    PubMed

    Polf, J C; Peterson, S; Ciangaru, G; Gillin, M; Beddar, S

    2009-02-07

    In this paper, we present the results of a preliminary study of secondary 'prompt' gamma-ray emission produced by proton-nuclear interactions within tissue during proton radiotherapy. Monte Carlo simulations were performed for mono-energetic proton beams, ranging from 2.5 MeV to 250 MeV, irradiating elemental and tissue targets. Calculations of the emission spectra from different biological tissues and their elemental components were made. Also, prompt gamma rays emitted during delivery of a clinical proton spread-out Bragg peak (SOBP) in a homogeneous water phantom and a water phantom containing heterogeneous tissue inserts were calculated to study the correlation between prompt gamma-ray production and proton dose delivery. The results show that the prompt gamma-ray spectra differ significantly for each type of tissue studied. The relative intensity of the characteristic gamma rays emitted from a given tissue was shown to be proportional to the concentration of each element in that tissue. A strong correlation was found between the delivered SOBP dose distribution and the characteristic prompt gamma-ray production. Based on these results, we discuss the potential use of prompt gamma-ray emission as a method to verify the accuracy and efficacy of doses delivered with proton radiotherapy.

  4. Identification of the glucose transporter in mammalian cell membranes using an /sup 125/(I)-forskolin photoaffinity label

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ruoho, A.; Wadzinski, B.; Shanahan, M.

    1987-05-01

    The glucose transporter has been identified in a variety of mammlian cell membranes using a carrier-free photoactivatable radioiodinated derivative of forskolin, 3-iodo-4-azidophenethylamido-7-0-succinyldeacetyl-forskolin, (I-125)IAPS-Fsk, at 1-10 nM. The membranes which have been photolabeled with (I-125)IAPS-Fsk are: rat cardiac sarcolemmal membranes, rat cortex and cerebellum synaptic membranes, human placental membranes, and wild type S49 lymphoma cell membranes. The glucose transporter in rat cardiac sarcolemmal membranes and rat cortex and cerebellum synaptic membranes was determined to be 45 kDa by SDS-PAGE. Photolysis of human placental membranes and S49 lymphoma membranes with (I-125)IAPS-Fsk followed by SDS-PAGE indicated specific derivatization of a broad band (45-55more » kDa) in placental membranes and a narrower band (45 kDa) in the S49 lymphoma membranes. Digestion of the (I-125)IPAS-Fsk labelled placental and S49 lymphoma membranes with endo-B-galactosidase showed a reduction in the apparent molecular weight of the radiolabelled band to 40 kDa. Trypsinization of labelled placental and lymphoma membranes produced an 18 kDa radiolabelled proteolytic fragment. (I-125)IAPS-Fsk is a highly effective probe for identifying low levels of glucose transporters in mammalian tissues.« less

  5. Development of an alpha/beta/gamma detector for radiation monitoring

    NASA Astrophysics Data System (ADS)

    Yamamoto, Seiichi; Hatazawa, Jun

    2011-11-01

    For radiation monitoring at the site of nuclear power plant accidents such as Fukushima Daiichi, radiation detectors not only for gamma photons but also for alpha and beta particles are needed because some nuclear fission products emit beta particles and gamma photons and some nuclear fuels contain plutonium that emits alpha particles. We developed a radiation detector that can simultaneously monitor alpha and beta particles and gamma photons for radiation monitoring. The detector consists of three-layered scintillators optically coupled to each other and coupled to a photomultiplier tube. The first layer, which is made of a thin plastic scintillator (decay time: 2.4 ns), detects alpha particles. The second layer, which is made of a thin Gd2SiO5 (GSO) scintillator with 1.5 mol.% Ce (decay time: 35 ns), detects beta particles. The third layer made of a thin GSO scintillator with 0.4 mol.% Ce (decay time: 70 ns) detects gamma photons. By using pulse shape discrimination, the count rates of these layers can be separated. With individual irradiation of alpha and beta particles and gamma photons, the count rate of the first layer represented the alpha particles, the second layer represented the beta particles, and the third layer represented the gamma photons. Even with simultaneous irradiation of the alpha and beta particles and the gamma photons, these three types of radiation can be individually monitored using correction for the gamma detection efficiency of the second and third layers. Our developed alpha, beta, and gamma detector is simple and will be useful for radiation monitoring, especially at nuclear power plant accident sites or other applications where the simultaneous measurements of alpha and beta particles and gamma photons are required.

  6. Development of an alpha/beta/gamma detector for radiation monitoring.

    PubMed

    Yamamoto, Seiichi; Hatazawa, Jun

    2011-11-01

    For radiation monitoring at the site of nuclear power plant accidents such as Fukushima Daiichi, radiation detectors not only for gamma photons but also for alpha and beta particles are needed because some nuclear fission products emit beta particles and gamma photons and some nuclear fuels contain plutonium that emits alpha particles. We developed a radiation detector that can simultaneously monitor alpha and beta particles and gamma photons for radiation monitoring. The detector consists of three-layered scintillators optically coupled to each other and coupled to a photomultiplier tube. The first layer, which is made of a thin plastic scintillator (decay time: 2.4 ns), detects alpha particles. The second layer, which is made of a thin Gd(2)SiO(5) (GSO) scintillator with 1.5 mol.% Ce (decay time: 35 ns), detects beta particles. The third layer made of a thin GSO scintillator with 0.4 mol.% Ce (decay time: 70 ns) detects gamma photons. By using pulse shape discrimination, the count rates of these layers can be separated. With individual irradiation of alpha and beta particles and gamma photons, the count rate of the first layer represented the alpha particles, the second layer represented the beta particles, and the third layer represented the gamma photons. Even with simultaneous irradiation of the alpha and beta particles and the gamma photons, these three types of radiation can be individually monitored using correction for the gamma detection efficiency of the second and third layers. Our developed alpha, beta, and gamma detector is simple and will be useful for radiation monitoring, especially at nuclear power plant accident sites or other applications where the simultaneous measurements of alpha and beta particles and gamma photons are required. © 2011 American Institute of Physics

  7. Top-emitting organic light-emitting diodes.

    PubMed

    Hofmann, Simone; Thomschke, Michael; Lüssem, Björn; Leo, Karl

    2011-11-07

    We review top-emitting organic light-emitting diodes (OLEDs), which are beneficial for lighting and display applications, where non-transparent substrates are used. The optical effects of the microcavity structure as well as the loss mechanisms are discussed. Outcoupling techniques and the work on white top-emitting OLEDs are summarized. We discuss the power dissipation spectra for a monochrome and a white top-emitting OLED and give quantitative reports on the loss channels. Furthermore, the development of inverted top-emitting OLEDs is described.

  8. Arcsec source location measurements in gamma-ray astronomy from a lunar observatory

    NASA Astrophysics Data System (ADS)

    Koch, D. G.; Hughes, B. E.

    1990-03-01

    The physical processes typically used in the detection of high energy gamma-rays do not permit good angular resolution, which makes difficult the unambiguous association of discrete gamma-ray sources with objects emitting at other wavelengths. This problem can be overcome by placing gamma-ray detectors on the moon and using the horizon as an occulting edge to achieve arcsec resolution. For the purpose of discussion, this concept is examined for gamma rays above about 20 MeV for which pair production dominates the detection process and locally-generated nuclear gamma rays do not contribute to the background.

  9. 10 CFR 35.655 - Five-year inspection for teletherapy and gamma stereotactic radiosurgery units.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Five-year inspection for teletherapy and gamma stereotactic radiosurgery units. 35.655 Section 35.655 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and Gamma Stereotactic...

  10. 10 CFR 35.655 - Five-year inspection for teletherapy and gamma stereotactic radiosurgery units.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Five-year inspection for teletherapy and gamma stereotactic radiosurgery units. 35.655 Section 35.655 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and Gamma Stereotactic...

  11. 10 CFR 35.655 - Five-year inspection for teletherapy and gamma stereotactic radiosurgery units.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Five-year inspection for teletherapy and gamma stereotactic radiosurgery units. 35.655 Section 35.655 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and Gamma Stereotactic...

  12. 10 CFR 35.655 - Five-year inspection for teletherapy and gamma stereotactic radiosurgery units.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Five-year inspection for teletherapy and gamma stereotactic radiosurgery units. 35.655 Section 35.655 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and Gamma Stereotactic...

  13. 10 CFR 35.655 - Five-year inspection for teletherapy and gamma stereotactic radiosurgery units.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Five-year inspection for teletherapy and gamma stereotactic radiosurgery units. 35.655 Section 35.655 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and Gamma Stereotactic...

  14. Structure of thermal pair clouds around gamma-ray-emitting black holes

    NASA Technical Reports Server (NTRS)

    Liang, Edison P.

    1991-01-01

    Using certain simplifying assumptions, the general structure of a quasi-spherical thermal pair-balanced cloud surrounding an accreting black hole is derived from first principles. Pair-dominated hot solutions exist only for a restricted range of the viscosity parameter. These results are applied as examples to the 1979 HEAO 3 gamma-ray data of Cygnus X-1 and the Galactic center. Values are obtained for the viscosity parameter lying in the range of about 0.1-0.01. Since the lack of synchrotron soft photons requires the magnetic field to be typically less than 1 percent of the equipartition value, a magnetic field cannot be the main contributor to the viscous stress of the inner accretion flow, at least during the high gamma-ray states.

  15. In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy

    PubMed Central

    Dudhia, Jayesh; Becerra, Patricia; Valdés, Miguel A.; Neves, Francisco; Hartman, Neil G.; Smith, Roger K.W.

    2015-01-01

    Recent advances in the application of bone marrow mesenchymal stem cells (BMMSC) for the treatment of tendon and ligament injuries in the horse suggest improved outcome measures in both experimental and clinical studies. Although the BMMSC are implanted into the tendon lesion in large numbers (usually 10 - 20 million cells), only a relatively small number survive (<10%) although these can persist for up to 5 months after implantation. This appears to be a common observation in other species where BMMSC have been implanted into other tissues and it is important to understand when this loss occurs, how many survive the initial implantation process and whether the cells are cleared into other organs. Tracking the fate of the cells can be achieved by radiolabeling the BMMSC prior to implantation which allows non-invasive in vivo imaging of cell location and quantification of cell numbers. This protocol describes a cell labeling procedure that uses Technetium-99m (Tc-99m), and tracking of these cells following implantation into injured flexor tendons in horses. Tc-99m is a short-lived (t1/2 of 6.01 hr) isotope that emits gamma rays and can be internalized by cells in the presence of the lipophilic compound hexamethylpropyleneamine oxime (HMPAO). These properties make it ideal for use in nuclear medicine clinics for the diagnosis of many different diseases. The fate of the labeled cells can be followed in the short term (up to 36 hr) by gamma scintigraphy to quantify both the number of cells retained in the lesion and distribution of the cells into lungs, thyroid and other organs. This technique is adapted from the labeling of blood leukocytes and could be utilized to image implanted BMMSC in other organs. PMID:26709915

  16. PULSAR OUTER-GAP ELECTRODYNAMICS: HARDENING OF SPECTRAL SHAPE IN THE TRAILING PEAK IN THE GAMMA-RAY LIGHT CURVE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hirotani, Kouichi, E-mail: hirotani@tiara.sinica.edu.tw

    2011-06-01

    The spectral characteristics of the pulsed gamma-ray emission from outer-magnetospheric particle accelerators are investigated. Either positrons or electrons are accelerated outward by the magnetic-field-aligned electric field to emit gamma rays via the curvature process. Since the particles move along relatively straight paths in the trailing side of a rotating magnetosphere, they attain higher Lorentz factors to emit more energetic gamma rays than those in the leading side. It is first demonstrated that the cutoff energy of the curvature radiation evolves with the rotation phase owing to the variation of the curvature radii of the particle paths and maximizes at amore » slightly later phase of the trailing peak in the gamma-ray light curve.« less

  17. Viability and proliferation potential of adipose-derived stem cells following labeling with a positron-emitting radiotracer.

    PubMed

    Elhami, Esmat; Goertzen, Andrew L; Xiang, Bo; Deng, Jixian; Stillwell, Chris; Mzengeza, Shadreck; Arora, Rakesh C; Freed, Darren; Tian, Ganghong

    2011-07-01

    Adipose-derived stem cells (ASCs) have promising potential in regenerative medicine and cell therapy. Our objective is to examine the biological function of the labeled stem cells following labeling with a readily available positron emission tomography (PET) tracer, (18)F-fluoro-2-deoxy-D: -glucose (FDG). In this work we characterize labeling efficiency through assessment of FDG uptake and retention by the ASCs and the effect of FDG on cell viability, proliferation, transdifferentiation, and cell function in vitro using rat ASCs. Samples of 10(5) ASCs (from visceral fat tissue) were labeled with concentrations of FDG (1-55 Bq/cell) in 0.75 ml culture medium. Label uptake and retention, as a function of labeling time, FDG concentration, and efflux period were measured to determine optimum cell labeling conditions. Cell viability, proliferation, DNA structure damage, cell differentiation, and other cell functions were examined. Non-labeled ASC samples were used as a control for all experimental groups. Labeled ASCs were injected via tail vein in several healthy rats and initial cell biodistribution was assessed. Our results showed that FDG uptake and retention by the stem cells did not depend on FDG concentration but on labeling and efflux periods and glucose content of the labeling and efflux media. Cell viability, transdifferentiation, and cell function were not greatly affected. DNA damage due to FDG radioactivity was acute, but reversible; cells managed to repair the damage and continue with cell cycles. Over all, FDG (up to 25 Bq/cell) did not impose severe cytotoxicity in rat ASCs. Initial biodistribution of the FDG-labeled ASCs was 80% + retention in the lungs. In the delayed whole-body images (2-3 h postinjection) there was some activity distribution resembling typical FDG uptake patterns. For in vivo cell tracking studies with PET tracers, the parameter of interest is the amount of radiotracer that is present in the cells being labeled and consequent

  18. Radiography apparatus using gamma rays emitted by water activated by fusion neutrons

    DOEpatents

    Smith, Donald L.; Ikeda, Yujiro; Uno, Yoshitomo

    1996-01-01

    Radiography apparatus includes an arrangement for circulating pure water continuously between a location adjacent a source of energetic neutrons, such as a tritium target irradiated by a deuteron beam, and a remote location where radiographic analysis is conducted. Oxygen in the pure water is activated via the .sup.16 O(n,p).sup.16 N reaction using .sup.14 -MeV neutrons produced at the neutron source via the .sup.3 H(d,n).sup.4 He reaction. Essentially monoenergetic gamma rays at 6.129 (predominantly) and 7.115 MeV are produced by the 7.13-second .sup.16 N decay for use in radiographic analysis. The gamma rays have substantial penetrating power and are useful in determining the thickness of materials and elemental compositions, particularly for metals and high-atomic number materials. The characteristic decay half life of 7.13 seconds of the activated oxygen is sufficient to permit gamma ray generation at a remote location where the activated water is transported, while not presenting a chemical or radioactivity hazard because the radioactivity falls to negligible levels after 1-2 minutes.

  19. Far-Red Fluorescent Lipid-Polymer Probes for an Efficient Labeling of Enveloped Viruses.

    PubMed

    Lacour, William; Adjili, Salim; Blaising, Julie; Favier, Arnaud; Monier, Karine; Mezhoud, Sarra; Ladavière, Catherine; Place, Christophe; Pécheur, Eve-Isabelle; Charreyre, Marie-Thérèse

    2016-08-01

    Far-red emitting fluorescent lipid probes are desirable to label enveloped viruses, for their efficient tracking by optical microscopy inside autofluorescent cells. Most used probes are rapidly released from membranes, leading to fluorescence signal decay and loss of contrast. Here, water-soluble lipid-polymer probes are synthesized harboring hydrophilic or hydrophobic far-red emitting dyes, and exhibiting enhanced brightness. They efficiently label Hepatitis C Virus pseudotyped particles (HCVpp), more stably and reproducibly than commercial probes, and a strong fluorescence signal is observed with a high contrast. Labeling with such probes do not alter virion morphology, integrity, nor infectivity. Finally, it is shown by fluorescence microscopy that these probes enable efficient tracking of labeled HCVpp inside hepatocarcinoma cells used as model hepatocytes, in spite of their autofluorescence up to 700 nm. These novel fluorescent lipid-polymer probes should therefore enable a better characterization of early stages of infection of autofluorescent cells by enveloped viruses. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. gamma. -Preprotachykinin-(72-92)-peptide amide: An endogenous preprotachykinin I gene-derived peptide that preferentially binds to neurokinin-2 receptors

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dam, T.V.; Takeda, Y.; Krause, J.E.

    1990-01-01

    The presence of N-terminally extended forms of neurokinin A has recently been reported in the mammalian brain. Among them, gamma-preprotachykinin-(72-92)-peptide amide (gamma-PPT-(72-92)-NH2), a peptide derived by posttranslational processing of gamma-preprotachykinin, is most prominent. We report here that this peptide most likely acts on neurokinin-2 receptor sites since neurokinin A (a putative neurokinin-2 agonist) and gamma-PPT-(72-92)-NH2 are potent competitors of 125I-labeled gamma-PPT-(72-92)-NH2 binding whereas selective neurokinin-1 and -3 agonists are not. Moreover, the distribution of 125I-labeled gamma-PPT-(72-92)-NH2 and 125I-labeled neurokinin A binding sites are very similar in rat brain. On the other hand, 125I-labeled Bolton-Hunter-substance P (a neurokinin-1 ligand) and 125I-labeledmore » Bolton-Hunter-eledoisin (a neurokinin-3 ligand) binding sites are differentially located in this tissue. Thus, it appears that gamma-PPT-(72-92)-NH2 binds to neurokinin-2 receptors and should be considered as a putative endogenous ligand for this receptor class.« less

  1. Positron emitter labeled enzyme inhibitors

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fowler, J.S.; MacGregor, R.R.; Wolf, A.P.

    This invention involves a new strategy for imaging and mapping enzyme activity in the living human and animal body using positron emitter-labeled suicide enzyme inactivators or inhibitors which become covalently bound to the enzyme as a result of enzymatic catalysis. Two such suicide inactivators for monoamine oxidase have been labeled with carbon-11 and used to map the enzyme subtypes in the living human and animal body using PET. By using positron emission tomography to image the distribution of radioactivity produced by the body penetrating radiation emitted by carbon-11, a map of functionally active monoamine oxidase activity is obtained. Clorgyline andmore » L-deprenyl are suicide enzyme inhibitors and irreversibly inhibit monoamine oxidase. When these inhibitors are labeled with carbon-11 they provide selective probes for monoamine oxidase localization and reactivity in vivo using positron emission tomography.« less

  2. Positron emitter labeled enzyme inhibitors

    DOEpatents

    Fowler, J.S.; MacGregor, R.R.; Wolf, A.P.

    1987-05-22

    This invention involved a new strategy for imaging and mapping enzyme activity in the living human and animal body using positron emitter-labeled suicide enzyme inactivators or inhibitors which become covalently bound to the enzyme as a result of enzymatic catalysis. Two such suicide in activators for monoamine oxidase have been labeled with carbon-11 and used to map the enzyme subtypes in the living human and animal body using PET. By using positron emission tomography to image the distribution of radioactivity produced by the body penetrating radiation emitted by carbon-11, a map of functionally active monoamine oxidase activity is obtained. Clorgyline and L-deprenyl are suicide enzyme inhibitors and irreversibly inhibit monoamine oxidase. When these inhibitors are labeled with carbon-11 they provide selective probes for monoamine oxidase localization and reactivity in vivo using positron emission tomography. 2 figs.

  3. Positron emitter labeled enzyme inhibitors

    DOEpatents

    Fowler, Joanna S.; MacGregor, Robert R.; Wolf, Alfred P.; Langstrom, Bengt

    1990-01-01

    This invention involves a new strategy for imaging and mapping enzyme activity in the living human and animal body using positron emitter-labeled suicide enzyme inactivators or inhibitors which become covalently bound to the enzyme as a result of enzymatic catalysis. Two such suicide inactivators for monoamine oxidase have been labeled with carbon-11 and used to map the enzyme subtypes in the living human and animal body using PET. By using positron emission tomography to image the distribution of radioactivity produced by the body penetrating radiation emitted by carbon-11, a map of functionally active monoamine oxidase activity is obtained. Clorgyline and L-deprenyl are suicide enzyme inhibitors and irreversibly inhibit monoamine oxidase. When these inhibitors are labeled with carbon-11 they provide selective probes for monoamine oxidase localization and reactivity in vivo using positron emission tomography.

  4. Prospects for future very high-energy gamma-ray sky survey: Impact of secondary gamma rays

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Inoue, Yoshiyuki; Kalashev, Oleg E.; Kusenko, Alexander

    2014-02-01

    Very high-energy gamma-ray measurements of distant blazars can be well explained by secondary gamma rays emitted by cascades induced by ultra-high-energy cosmic rays. The secondary gamma rays will enable one to detect a large number of blazars with future ground based gamma-ray telescopes such as Cherenkov Telescope Array (CTA). We show that the secondary emission process will allow CTA to detect 100, 130, 150, 87, and 8 blazars above 30 GeV, 100 GeV, 300 GeV, 1 TeV, and 10 TeV, respectively, up to z~8 assuming the intergalactic magnetic field (IGMF) strength B=10-17 G and an unbiased all sky survey withmore » 0.5 h exposure at each field of view, where total observing time is ~540 h. These numbers will be 79, 96, 110, 63, and 6 up to z~5 in the case of B=10-15 G. This large statistics of sources will be a clear evidence of the secondary gamma-ray scenarios and a new key to studying the IGMF statistically. We also find that a wider and shallower survey is favored to detect more and higher redshift sources even if we take into account secondary gamma rays.« less

  5. Measurement of characteristic prompt gamma rays emitted from oxygen and carbon in tissue-equivalent samples during proton beam irradiation

    PubMed Central

    Polf, Jerimy C; Panthi, Rajesh; Mackin, Dennis S; McCleskey, Matt; Saastamoinen, Antti; Roeder, Brian T; Beddar, Sam

    2013-01-01

    The purpose of this work was to characterize how prompt gamma (PG) emission from tissue changes as a function of carbon and oxygen concentration, and to assess the feasibility of determining elemental concentration in tissues irradiated with proton beams. For this study, four tissue-equivalent water-sucrose samples with differing densities and concentrations of carbon, hydrogen, and oxygen were irradiated with a 48 MeV proton pencil beam. The PG spectrum emitted from each sample was measured using a high-purity germanium detector, and the absolute detection efficiency of the detector, average beam current, and delivered dose distribution were also measured. Changes to the total PG emission from 12C (4.44 MeV) and 16O (6.13 MeV) per incident proton and per Gray of absorbed dose were characterized as a function of carbon and oxygen concentration in the sample. The intensity of the 4.44 MeV PG emission per incident proton was found to be nearly constant for all samples regardless of their carbon concentration. However, we found that the 6.13 MeV PG emission increased linearly with the total amount (in grams) of oxygen irradiated in the sample. From the measured PG data, we determined that 1.64 × 107 oxygen PGs were emitted per gram of oxygen irradiated per Gray of absorbed dose delivered with a 48 MeV proton beam. These results indicate that the 6.13 MeV PG emission from 16O is proportional to the concentration of oxygen in tissue irradiated with proton beams, showing that it is possible to determine the concentration of oxygen within tissues irradiated with proton beams by measuring 16O PG emission. PMID:23920051

  6. Radiography apparatus using gamma rays emitted by water activated by fusion neutrons

    DOEpatents

    Smith, D.L.; Ikeda, Yujiro; Uno, Yoshitomo

    1996-11-05

    Radiography apparatus includes an arrangement for circulating pure water continuously between a location adjacent a source of energetic neutrons, such as a tritium target irradiated by a deuteron beam, and a remote location where radiographic analysis is conducted. Oxygen in the pure water is activated via the {sup 16}O(n,p){sup 16}N reaction using {sup 14}N-MeV neutrons produced at the neutron source via the {sup 3}H(d,n){sup 4}He reaction. Essentially monoenergetic gamma rays at 6.129 (predominantly) and 7.115 MeV are produced by the 7.13-second {sup 16}N decay for use in radiographic analysis. The gamma rays have substantial penetrating power and are useful in determining the thickness of materials and elemental compositions, particularly for metals and high-atomic number materials. The characteristic decay half life of 7.13 seconds of the activated oxygen is sufficient to permit gamma ray generation at a remote location where the activated water is transported, while not presenting a chemical or radioactivity hazard because the radioactivity falls to negligible levels after 1--2 minutes. 15 figs.

  7. The interaction of substituted benzamides with brain benzodiazepine binding sites in vitro.

    PubMed

    Horton, R W; Lowther, S; Chivers, J; Jenner, P; Marsden, C D; Testa, B

    1988-08-01

    1. The interaction of substituted benzamides with brain benzodiazepine (BDZ) binding sites was examined by their ability to displace [3H]-flunitrazepam ([3H]-FNM) from specific binding sites in bovine cortical membranes in vitro. 2. Clebopride, Delagrange 2674, Delagrange 2335 and BRL 20627 displayed concentration-dependent displacement of [3H]-FNM with IC50 values of 73 nM, 132 nM, 7.7 microM and 5.9 microM, respectively. Other substituted benzamides including metoclopramide, sulpiride, tiapride, sultopride and cisapride were inactive at 10(-5) M. 3. Inhibition by clebopride and Delagrange 2674 of [3H]-FNM binding was apparently competitive and readily reversible. 4. In the presence of gamma-aminobutyric acid (GABA), the ability of diazepam and Delagrange 2674 to displace [3H]-Ro 15-1788 binding was increased 3.6 and 1.6 fold respectively, compared to the absence of GABA, while ethyl beta-carboline-3-carboxylate (beta CCE) and clebopride were less potent in the presence of GABA. 5. Diazepam was 30 fold less potent at displacing [3H]-Ro 15-1788 in membranes that had been photoaffinity labelled with FNM than in control membranes, whereas the potency of beta CCE did not differ. Clebopride and Delagrange 2674 showed a less than two fold loss of potency in photoaffinity labelled membranes. 6. The pattern of binding of clebopride and Delagrange 2674 in these in vitro tests is similar to that found previously with partial agonists or antagonists at BDZ binding sites. 7. Clebopride and Delagrange 2674 inhibited [3H]-FNM binding with similar potency in rat cerebellar and hippocampal membranes, suggesting they have no selectivity for BDZ1 and BDZ2 binding sites. 8. Clebopride and Delagrange 2674 are structurally dissimilar to other BDZ ligands and represent another chemical structure to probe brain BDZ binding sites.

  8. The interaction of substituted benzamides with brain benzodiazepine binding sites in vitro.

    PubMed Central

    Horton, R. W.; Lowther, S.; Chivers, J.; Jenner, P.; Marsden, C. D.; Testa, B.

    1988-01-01

    1. The interaction of substituted benzamides with brain benzodiazepine (BDZ) binding sites was examined by their ability to displace [3H]-flunitrazepam ([3H]-FNM) from specific binding sites in bovine cortical membranes in vitro. 2. Clebopride, Delagrange 2674, Delagrange 2335 and BRL 20627 displayed concentration-dependent displacement of [3H]-FNM with IC50 values of 73 nM, 132 nM, 7.7 microM and 5.9 microM, respectively. Other substituted benzamides including metoclopramide, sulpiride, tiapride, sultopride and cisapride were inactive at 10(-5) M. 3. Inhibition by clebopride and Delagrange 2674 of [3H]-FNM binding was apparently competitive and readily reversible. 4. In the presence of gamma-aminobutyric acid (GABA), the ability of diazepam and Delagrange 2674 to displace [3H]-Ro 15-1788 binding was increased 3.6 and 1.6 fold respectively, compared to the absence of GABA, while ethyl beta-carboline-3-carboxylate (beta CCE) and clebopride were less potent in the presence of GABA. 5. Diazepam was 30 fold less potent at displacing [3H]-Ro 15-1788 in membranes that had been photoaffinity labelled with FNM than in control membranes, whereas the potency of beta CCE did not differ. Clebopride and Delagrange 2674 showed a less than two fold loss of potency in photoaffinity labelled membranes. 6. The pattern of binding of clebopride and Delagrange 2674 in these in vitro tests is similar to that found previously with partial agonists or antagonists at BDZ binding sites. 7. Clebopride and Delagrange 2674 inhibited [3H]-FNM binding with similar potency in rat cerebellar and hippocampal membranes, suggesting they have no selectivity for BDZ1 and BDZ2 binding sites.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2850059

  9. Apparatus and method for detecting gamma radiation

    DOEpatents

    Sigg, R.A.

    1994-12-13

    A high efficiency radiation detector is disclosed for measuring X-ray and gamma radiation from small-volume, low-activity liquid samples with an overall uncertainty better than 0.7% (one sigma SD). The radiation detector includes a hyperpure germanium well detector, a collimator, and a reference source. The well detector monitors gamma radiation emitted by the reference source and a radioactive isotope or isotopes in a sample source. The radiation from the reference source is collimated to avoid attenuation of reference source gamma radiation by the sample. Signals from the well detector are processed and stored, and the stored data is analyzed to determine the radioactive isotope(s) content of the sample. Minor self-attenuation corrections are calculated from chemical composition data. 4 figures.

  10. Apparatus and method for detecting gamma radiation

    DOEpatents

    Sigg, Raymond A.

    1994-01-01

    A high efficiency radiation detector for measuring X-ray and gamma radiation from small-volume, low-activity liquid samples with an overall uncertainty better than 0.7% (one sigma SD). The radiation detector includes a hyperpure germanium well detector, a collimator, and a reference source. The well detector monitors gamma radiation emitted by the reference source and a radioactive isotope or isotopes in a sample source. The radiation from the reference source is collimated to avoid attenuation of reference source gamma radiation by the sample. Signals from the well detector are processed and stored, and the stored data is analyzed to determine the radioactive isotope(s) content of the sample. Minor self-attenuation corrections are calculated from chemical composition data.

  11. Characterization of uranium bearing material using x-ray fluorescence and direct gamma-rays measurement techniques

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mujaini, M., E-mail: madihah@uniten.edu.my; Chankow, N.; Yusoff, M. Z.

    2016-01-22

    Uranium ore can be easily detected due to various gamma-ray energies emitted from uranium daughters particularly from {sup 238}U daughters such as {sup 214}Bi, {sup 214}Pb and {sup 226}Ra. After uranium is extracted from uranium ore, only low energy gamma-rays emitted from {sup 235}U may be detected if the detector is placed in close contact to the specimen. In this research, identification and characterization of uranium bearing materials is experimentally investigated using direct measurement of gamma-rays from {sup 235}U in combination with the x-ray fluorescence (XRF) technique. Measurement of gamma-rays can be conducted by using high purity germanium (HPGe) detectormore » or cadmium telluride (CdTe) detector while a {sup 57}Coradioisotope-excited XRF spectrometer using CdTe detector is used for elemental analysis. The proposed technique was tested with various uranium bearing specimens containing natural, depleted and enriched uranium in both metallic and powder forms.« less

  12. Gamma-ray flares from the Crab Nebula.

    PubMed

    Abdo, A A; Ackermann, M; Ajello, M; Allafort, A; Baldini, L; Ballet, J; Barbiellini, G; Bastieri, D; Bechtol, K; Bellazzini, R; Berenji, B; Blandford, R D; Bloom, E D; Bonamente, E; Borgland, A W; Bouvier, A; Brandt, T J; Bregeon, J; Brez, A; Brigida, M; Bruel, P; Buehler, R; Buson, S; Caliandro, G A; Cameron, R A; Cannon, A; Caraveo, P A; Casandjian, J M; Çelik, Ö; Charles, E; Chekhtman, A; Cheung, C C; Chiang, J; Ciprini, S; Claus, R; Cohen-Tanugi, J; Costamante, L; Cutini, S; D'Ammando, F; Dermer, C D; de Angelis, A; de Luca, A; de Palma, F; Digel, S W; do Couto e Silva, E; Drell, P S; Drlica-Wagner, A; Dubois, R; Dumora, D; Favuzzi, C; Fegan, S J; Ferrara, E C; Focke, W B; Fortin, P; Frailis, M; Fukazawa, Y; Funk, S; Fusco, P; Gargano, F; Gasparrini, D; Gehrels, N; Germani, S; Giglietto, N; Giordano, F; Giroletti, M; Glanzman, T; Godfrey, G; Grenier, I A; Grondin, M-H; Grove, J E; Guiriec, S; Hadasch, D; Hanabata, Y; Harding, A K; Hayashi, K; Hayashida, M; Hays, E; Horan, D; Itoh, R; Jóhannesson, G; Johnson, A S; Johnson, T J; Khangulyan, D; Kamae, T; Katagiri, H; Kataoka, J; Kerr, M; Knödlseder, J; Kuss, M; Lande, J; Latronico, L; Lee, S-H; Lemoine-Goumard, M; Longo, F; Loparco, F; Lubrano, P; Madejski, G M; Makeev, A; Marelli, M; Mazziotta, M N; McEnery, J E; Michelson, P F; Mitthumsiri, W; Mizuno, T; Moiseev, A A; Monte, C; Monzani, M E; Morselli, A; Moskalenko, I V; Murgia, S; Nakamori, T; Naumann-Godo, M; Nolan, P L; Norris, J P; Nuss, E; Ohsugi, T; Okumura, A; Omodei, N; Ormes, J F; Ozaki, M; Paneque, D; Parent, D; Pelassa, V; Pepe, M; Pesce-Rollins, M; Pierbattista, M; Piron, F; Porter, T A; Rainò, S; Rando, R; Ray, P S; Razzano, M; Reimer, A; Reimer, O; Reposeur, T; Ritz, S; Romani, R W; Sadrozinski, H F-W; Sanchez, D; Saz Parkinson, P M; Scargle, J D; Schalk, T L; Sgrò, C; Siskind, E J; Smith, P D; Spandre, G; Spinelli, P; Strickman, M S; Suson, D J; Takahashi, H; Takahashi, T; Tanaka, T; Thayer, J B; Thompson, D J; Tibaldo, L; Torres, D F; Tosti, G; Tramacere, A; Troja, E; Uchiyama, Y; Vandenbroucke, J; Vasileiou, V; Vianello, G; Vitale, V; Wang, P; Wood, K S; Yang, Z; Ziegler, M

    2011-02-11

    A young and energetic pulsar powers the well-known Crab Nebula. Here, we describe two separate gamma-ray (photon energy greater than 100 mega-electron volts) flares from this source detected by the Large Area Telescope on board the Fermi Gamma-ray Space Telescope. The first flare occurred in February 2009 and lasted approximately 16 days. The second flare was detected in September 2010 and lasted approximately 4 days. During these outbursts, the gamma-ray flux from the nebula increased by factors of four and six, respectively. The brevity of the flares implies that the gamma rays were emitted via synchrotron radiation from peta-electron-volt (10(15) electron volts) electrons in a region smaller than 1.4 × 10(-2) parsecs. These are the highest-energy particles that can be associated with a discrete astronomical source, and they pose challenges to particle acceleration theory.

  13. Gamma-Ray Flares from the Crab Nebula

    NASA Astrophysics Data System (ADS)

    Abdo, A. A.; Ackermann, M.; Ajello, M.; Allafort, A.; Baldini, L.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Bechtol, K.; Bellazzini, R.; Berenji, B.; Blandford, R. D.; Bloom, E. D.; Bonamente, E.; Borgland, A. W.; Bouvier, A.; Brandt, T. J.; Bregeon, J.; Brez, A.; Brigida, M.; Bruel, P.; Buehler, R.; Buson, S.; Caliandro, G. A.; Cameron, R. A.; Cannon, A.; Caraveo, P. A.; Casandjian, J. M.; Çelik, Ö.; Charles, E.; Chekhtman, A.; Cheung, C. C.; Chiang, J.; Ciprini, S.; Claus, R.; Cohen-Tanugi, J.; Costamante, L.; Cutini, S.; D'Ammando, F.; Dermer, C. D.; de Angelis, A.; de Luca, A.; de Palma, F.; Digel, S. W.; do Couto e Silva, E.; Drell, P. S.; Drlica-Wagner, A.; Dubois, R.; Dumora, D.; Favuzzi, C.; Fegan, S. J.; Ferrara, E. C.; Focke, W. B.; Fortin, P.; Frailis, M.; Fukazawa, Y.; Funk, S.; Fusco, P.; Gargano, F.; Gasparrini, D.; Gehrels, N.; Germani, S.; Giglietto, N.; Giordano, F.; Giroletti, M.; Glanzman, T.; Godfrey, G.; Grenier, I. A.; Grondin, M.-H.; Grove, J. E.; Guiriec, S.; Hadasch, D.; Hanabata, Y.; Harding, A. K.; Hayashi, K.; Hayashida, M.; Hays, E.; Horan, D.; Itoh, R.; Jóhannesson, G.; Johnson, A. S.; Johnson, T. J.; Khangulyan, D.; Kamae, T.; Katagiri, H.; Kataoka, J.; Kerr, M.; Knödlseder, J.; Kuss, M.; Lande, J.; Latronico, L.; Lee, S.-H.; Lemoine-Goumard, M.; Longo, F.; Loparco, F.; Lubrano, P.; Madejski, G. M.; Makeev, A.; Marelli, M.; Mazziotta, M. N.; McEnery, J. E.; Michelson, P. F.; Mitthumsiri, W.; Mizuno, T.; Moiseev, A. A.; Monte, C.; Monzani, M. E.; Morselli, A.; Moskalenko, I. V.; Murgia, S.; Nakamori, T.; Naumann-Godo, M.; Nolan, P. L.; Norris, J. P.; Nuss, E.; Ohsugi, T.; Okumura, A.; Omodei, N.; Ormes, J. F.; Ozaki, M.; Paneque, D.; Parent, D.; Pelassa, V.; Pepe, M.; Pesce-Rollins, M.; Pierbattista, M.; Piron, F.; Porter, T. A.; Rainò, S.; Rando, R.; Ray, P. S.; Razzano, M.; Reimer, A.; Reimer, O.; Reposeur, T.; Ritz, S.; Romani, R. W.; Sadrozinski, H. F.-W.; Sanchez, D.; Parkinson, P. M. Saz; Scargle, J. D.; Schalk, T. L.; Sgrò, C.; Siskind, E. J.; Smith, P. D.; Spandre, G.; Spinelli, P.; Strickman, M. S.; Suson, D. J.; Takahashi, H.; Takahashi, T.; Tanaka, T.; Thayer, J. B.; Thompson, D. J.; Tibaldo, L.; Torres, D. F.; Tosti, G.; Tramacere, A.; Troja, E.; Uchiyama, Y.; Vandenbroucke, J.; Vasileiou, V.; Vianello, G.; Vitale, V.; Wang, P.; Wood, K. S.; Yang, Z.; Ziegler, M.

    2011-02-01

    A young and energetic pulsar powers the well-known Crab Nebula. Here, we describe two separate gamma-ray (photon energy greater than 100 mega-electron volts) flares from this source detected by the Large Area Telescope on board the Fermi Gamma-ray Space Telescope. The first flare occurred in February 2009 and lasted approximately 16 days. The second flare was detected in September 2010 and lasted approximately 4 days. During these outbursts, the gamma-ray flux from the nebula increased by factors of four and six, respectively. The brevity of the flares implies that the gamma rays were emitted via synchrotron radiation from peta-electron-volt (1015 electron volts) electrons in a region smaller than 1.4 × 10-2 parsecs. These are the highest-energy particles that can be associated with a discrete astronomical source, and they pose challenges to particle acceleration theory.

  14. Sensitivity to Antibiotics of Bacteria Exposed to Gamma Radiation Emitted from Hot Soils of the High Background Radiation Areas of Ramsar, Northern Iran.

    PubMed

    Mortazavi, Seyed Mohammad Javad; Zarei, Samira; Taheri, Mohammad; Tajbakhsh, Saeed; Mortazavi, Seyed Alireza; Ranjbar, Sahar; Momeni, Fatemeh; Masoomi, Samaneh; Ansari, Leila; Movahedi, Mohammad Mehdi; Taeb, Shahram; Zarei, Sina; Haghani, Masood

    2017-04-01

    Over the past several years our laboratories have investigated different aspects of the challenging issue of the alterations in bacterial susceptibility to antibiotics induced by physical stresses. To explore the bacterial susceptibility to antibiotics in samples of Salmonella enterica subsp. enterica serovar Typhimurium ( S. typhimurium ), Staphylococcus aureus , and Klebsiella pneumoniae after exposure to gamma radiation emitted from the soil samples taken from the high background radiation areas of Ramsar, northern Iran. Standard Kirby-Bauer test, which evaluates the size of the zone of inhibition as an indicator of the susceptibility of different bacteria to antibiotics, was used in this study. The maximum alteration of the diameter of inhibition zone was found for K. pneumoniae when tested for ciprofloxacin. In this case, the mean diameter of no growth zone in non-irradiated control samples of K. pneumoniae was 20.3 (SD 0.6) mm; it was 14.7 (SD 0.6) mm in irradiated samples. On the other hand, the minimum changes in the diameter of inhibition zone were found for S. typhimurium and S. aureus when these bacteria were tested for nitrofurantoin and cephalexin, respectively. Gamma rays were capable of making significant alterations in bacterial susceptibility to antibiotics. It can be hypothesized that high levels of natural background radiation can induce adaptive phenomena that help microorganisms better cope with lethal effects of antibiotics.

  15. Detection of high-energy gamma-ray emission from the globular cluster 47 Tucanae with Fermi.

    PubMed

    Abdo, A A; Ackermann, M; Ajello, M; Atwood, W B; Axelsson, M; Baldini, L; Ballet, J; Barbiellini, G; Bastieri, D; Baughman, B M; Bechtol, K; Bellazzini, R; Berenji, B; Blandford, R D; Bloom, E D; Bonamente, E; Borgland, A W; Bregeon, J; Brez, A; Brigida, M; Bruel, P; Burnett, T H; Caliandro, G A; Cameron, R A; Caraveo, P A; Casandjian, J M; Cecchi, C; Celik, O; Charles, E; Chaty, S; Chekhtman, A; Cheung, C C; Chiang, J; Ciprini, S; Claus, R; Cohen-Tanugi, J; Conrad, J; Cutini, S; Dermer, C D; de Palma, F; Digel, S W; Dormody, M; do Couto e Silva, E; Drell, P S; Dubois, R; Dumora, D; Farnier, C; Favuzzi, C; Fegan, S J; Focke, W B; Frailis, M; Fukazawa, Y; Fusco, P; Gargano, F; Gasparrini, D; Gehrels, N; Germani, S; Giebels, B; Giglietto, N; Giordano, F; Glanzman, T; Godfrey, G; Grenier, I A; Grove, J E; Guillemot, L; Guiriec, S; Hanabata, Y; Harding, A K; Hayashida, M; Hays, E; Horan, D; Hughes, R E; Jóhannesson, G; Johnson, A S; Johnson, R P; Johnson, T J; Johnson, W N; Kamae, T; Katagiri, H; Kawai, N; Kerr, M; Knödlseder, J; Kuehn, F; Kuss, M; Lande, J; Latronico, L; Lemoine-Goumard, M; Longo, F; Loparco, F; Lott, B; Lovellette, M N; Lubrano, P; Makeev, A; Mazziotta, M N; McConville, W; McEnery, J E; Meurer, C; Michelson, P F; Mitthumsiri, W; Mizuno, T; Moiseev, A A; Monte, C; Monzani, M E; Morselli, A; Moskalenko, I V; Murgia, S; Nolan, P L; Norris, J P; Nuss, E; Ohsugi, T; Omodei, N; Orlando, E; Ormes, J F; Paneque, D; Panetta, J H; Parent, D; Pelassa, V; Pepe, M; Pierbattista, M; Piron, F; Porter, T A; Rainò, S; Rando, R; Razzano, M; Rea, N; Reimer, A; Reimer, O; Reposeur, T; Ritz, S; Rochester, L S; Rodriguez, A Y; Romani, R W; Roth, M; Ryde, F; Sadrozinski, H F-W; Sanchez, D; Sander, A; Saz Parkinson, P M; Sgrò, C; Smith, D A; Smith, P D; Spandre, G; Spinelli, P; Starck, J-L; Strickman, M S; Suson, D J; Tajima, H; Takahashi, H; Tanaka, T; Thayer, J B; Thayer, J G; Thompson, D J; Tibaldo, L; Torres, D F; Tosti, G; Tramacere, A; Uchiyama, Y; Usher, T L; Vasileiou, V; Vilchez, N; Vitale, V; Wang, P; Webb, N; Winer, B L; Wood, K S; Ylinen, T; Ziegler, M

    2009-08-14

    We report the detection of gamma-ray emissions above 200 megaelectron volts at a significance level of 17sigma from the globular cluster 47 Tucanae, using data obtained with the Large Area Telescope onboard the Fermi Gamma-ray Space Telescope. Globular clusters are expected to emit gamma rays because of the large populations of millisecond pulsars that they contain. The spectral shape of 47 Tucanae is consistent with gamma-ray emission from a population of millisecond pulsars. The observed gamma-ray luminosity implies an upper limit of 60 millisecond pulsars present in 47 Tucanae.

  16. Verbal Labels Modulate Perceptual Object Processing in 1-Year-Old Children

    ERIC Educational Resources Information Center

    Gliga, Teodora; Volein, Agnes; Csibra, Gergely

    2010-01-01

    Whether verbal labels help infants visually process and categorize objects is a contentious issue. Using electroencephalography, we investigated whether possessing familiar or novel labels for objects directly enhances 1-year-old children's neural processes underlying the perception of those objects. We found enhanced gamma-band (20-60 Hz)…

  17. Positron emitter labeled enzyme inhibitors

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fowler, J.S.; MacGregor, R.R.; Wolf, A.P.

    This invention involved a new strategy for imaging and mapping enzyme activity in the living human and animal body using positron emitter-labeled suicide enzyme inactivators or inhibitors which become covalently bound to the enzyme as a result of enzymatic catalysis. Two such suicide in activators for monoamine oxidase have been labeled with carbon-11 and used to map the enzyme subtypes in the living human and animal body using PET. By using positron emission tomography to image the distribution of radioactivity produced by the body penetrating radiation emitted by carbon-11, a map of functionally active monoamine oxidase activity is obtained. Clorgylinemore » and L-deprenyl are suicide enzyme inhibitors and irreversibly inhibit monoamine oxidase. When these inhibitors are labeled with carbon-11 they provide selective probes for monoamine oxidase localization and reactivity in vivo using positron emission tomography. 2 figs.« less

  18. Specification of High Activity Gamma-Ray Sources.

    ERIC Educational Resources Information Center

    International Commission on Radiation Units and Measurements, Washington, DC.

    The report is concerned with making recommendations for the specifications of gamma ray sources, which relate to the quantity of radioactive material and the radiation emitted. Primary consideration is given to sources in teletherapy and to a lesser extent those used in industrial radiography and in irradiation units used in industry and research.…

  19. Distribution of artificial gamma-ray emitting radionuclide activity concentration in the top soil in the vicinity of the Ignalina Nuclear Power Plant and other regions in Lithuania.

    PubMed

    Lukšienė, Benedikta; Marčiulionienė, Danutė; Rožkov, Andrej; Gudelis, Arūnas; Holm, Elis; Galvonaitė, Audronė

    2012-11-15

    The impact of the operating Ignalina Nuclear Power Plant (INPP) on the contamination of top soil layer with artificial radionuclides has been studied. Results of the investigation of artificial gamma-ray emitting radionuclide distribution in soil in the vicinity of the INPP and distant regions in Lithuania in 1996-2008 (INPP operational period) show that nowadays (137)Cs remains the most important artificial gamma-ray emitting radionuclide in the upper soil layer. Mean (137)Cs activity concentrations in the top soil layer in the vicinity of the INPP were found to be significantly lower compared to those in remote regions of Varėna and Plungė (~300 km from INPP). In 1996 and 1998 mean (137)Cs activity concentrations were in the range of 28-45 Bq/kg in the nearest vicinity to the INPP, 103 Bq/kg in Varėna and 340 Bq/kg in Plungė region. (137)Cs activity concentrations were 5-20 times lower in meadow soil (4-14 Bq/kg) compared to swamp and forest soil. (60)Co, the INPP origin radionuclide, was detected in samples only in 1996 and 2000, and the activity concentration of (60)Co was found to be in the range from 0.4 to 7.0 Bq/kg at the sampling ground nearest to the INPP. Average annual activity concentrations of the INPP origin (137)Cs and (60)Co in the air and depositions in the INPP region were modeled using Pasquill-Gifford equations. The modeling results of (137)Cs and (60)Co depositional load in the INPP vicinity agree with the experimentally obtained values. Our results provide the evidence that the operation of INPP did not cause any significant contamination in soil surface. Copyright © 2012 Elsevier B.V. All rights reserved.

  20. Photoaffinity Labeling of the Plasmodium falciparum Chloroquine Resistance Transporter with a Novel Perfluorophenylazido Chloroquine†

    PubMed Central

    Lekostaj, Jacqueline K.; Natarajan, Jayakumar K.; Paguio, Michelle F.; Wolf, Christian; Roepe, Paul D.

    2009-01-01

    Several models describing how amino acid substitutions in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) confer resistance to chloroquine (CQ) and other antimalarial drugs have been proposed. Further progress requires molecular analysis of interactions between purified reconstituted PfCRT protein and these drugs. We have thus designed and synthesized several perfluorophenyl azido (pfpa) CQ analogues for PfCRT photolabeling studies. One particularly useful probe (AzBCQ) places the pfpa group at the terminal aliphatic N of CQ via a flexible four-carbon ester linker and includes a convenient biotin tag. This probe photolabels PfCRT in situ with high specificity. Using reconstituted proteoliposomes harboring partially purified recombinant PfCRT, we analyze AzBCQ photolabeling versus competition with CQ and other drugs to probe the nature of the CQ binding site. We also inspect how pH, the chemoreversal agent verapamil (VPL), and various amino acid mutations in PfCRT that cause CQ resistance (CQR) affect the efficiency of AzBCQ photolabeling. Upon gel isolation of AzBCQ-labeled PfCRT followed by trypsin digestion and mass spectrometry analysis, we are able to define a single AzBCQ covalent attachment site lying within the digestive vacuolar-disposed loop between putative helices 9 and 10 of PfCRT. Taken together, the data provide important new insight into PfCRT function and, along with previous results, allow us to propose a model for a single CQ binding site in the PfCRT protein. PMID:18767816

  1. Photoaffinity labeling of the Plasmodium falciparum chloroquine resistance transporter with a novel perfluorophenylazido chloroquine.

    PubMed

    Lekostaj, Jacqueline K; Natarajan, Jayakumar K; Paguio, Michelle F; Wolf, Christian; Roepe, Paul D

    2008-09-30

    Several models describing how amino acid substitutions in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) confer resistance to chloroquine (CQ) and other antimalarial drugs have been proposed. Further progress requires molecular analysis of interactions between purified reconstituted PfCRT protein and these drugs. We have thus designed and synthesized several perfluorophenyl azido (pfpa) CQ analogues for PfCRT photolabeling studies. One particularly useful probe (AzBCQ) places the pfpa group at the terminal aliphatic N of CQ via a flexible four-carbon ester linker and includes a convenient biotin tag. This probe photolabels PfCRT in situ with high specificity. Using reconstituted proteoliposomes harboring partially purified recombinant PfCRT, we analyze AzBCQ photolabeling versus competition with CQ and other drugs to probe the nature of the CQ binding site. We also inspect how pH, the chemoreversal agent verapamil (VPL), and various amino acid mutations in PfCRT that cause CQ resistance (CQR) affect the efficiency of AzBCQ photolabeling. Upon gel isolation of AzBCQ-labeled PfCRT followed by trypsin digestion and mass spectrometry analysis, we are able to define a single AzBCQ covalent attachment site lying within the digestive vacuolar-disposed loop between putative helices 9 and 10 of PfCRT. Taken together, the data provide important new insight into PfCRT function and, along with previous results, allow us to propose a model for a single CQ binding site in the PfCRT protein.

  2. Gamma-ray pulsars: Emission zones and viewing geometries

    NASA Technical Reports Server (NTRS)

    Romani, Roger W.; Yadigaroglu, I.-A.

    1995-01-01

    There are now a half-dozen young pulsars detected in high-energy photons by the Compton Gamma-Ray Observatory (CGRO), showing a variety of emission efficiencies and pulse profiles. We present here a calculation of the pattern of high-energy emission on the sky in a model which posits gamma-ray production by charge-depleted gaps in the outer magnetosphere. This model accounts for the radio to gamma-ray pulse offsets of the known pulsars, as well as the shape of the high-energy pulse profiles. We also show that about one-third of emitting young radio pulsars will not be detected due to beaming effects, while approximately 2.5 times the number of radio-selected gamma-ray pulsars will be viewed only high energies. Finally we compute the polarization angle variation and find that the previously misunderstood optical polarization sweep of the Crab pulsar arises naturally in this picture. These results strongly support an outer magnetosphere location for the gamma-ray emission.

  3. Gamma-ray blind beta particle probe

    DOEpatents

    Weisenberger, Andrew G.

    2001-01-01

    An intra-operative beta particle probe is provided by placing a suitable photomultiplier tube (PMT), micro channel plate (MCP) or other electron multiplier device within a vacuum housing equipped with: 1) an appropriate beta particle permeable window; and 2) electron detection circuitry. Beta particles emitted in the immediate vicinity of the probe window will be received by the electron multiplier device and amplified to produce a detectable signal. Such a device is useful as a gamma insensitive, intra-operative, beta particle probe in surgeries where the patient has been injected with a beta emitting radiopharmaceutical. The method of use of such a device is also described, as is a position sensitive such device.

  4. New Fermi-LAT event reconstruction reveals more high-energy gamma rays from gamma-ray bursts

    DOE PAGES

    Atwood, W. B.; Baldini, L.; Bregeon, J.; ...

    2013-08-19

    Here, based on the experience gained during the four and a half years of the mission, the Fermi-LAT Collaboration has undertaken a comprehensive revision of the event-level analysis going under the name of Pass 8. Although it is not yet finalized, we can test the improvements in the new event reconstruction with the special case of the prompt phase of bright gamma-ray bursts (GRBs), where the signal-to-noise ratio is large enough that loose selection cuts are sufficient to identify gamma rays associated with the source. Using the new event reconstruction, we have re-analyzed 10 GRBs previously detected by the Largemore » Area Telescope (LAT) for which an X-ray/optical follow-up was possible and found four new gamma rays with energies greater than 10 GeV in addition to the seven previously known. Among these four is a 27.4 GeV gamma ray from GRB 080916C, which has a redshift of 4.35, thus making it the gamma ray with the highest intrinsic energy (~147 GeV) detected from a GRB. We present here the salient aspects of the new event reconstruction and discuss the scientific implications of these new high-energy gamma rays, such as constraining extragalactic background light models, Lorentz invariance violation tests, the prompt emission mechanism, and the bulk Lorentz factor of the emitting region.« less

  5. Low-picomolar limits of detection using high-power light-emitting diodes for fluorescence.

    PubMed

    de Jong, Ebbing P; Lucy, Charles A

    2006-05-01

    Fluorescence detectors are ever more frequently being used with light-emitting diodes (LEDs) as the light source. Technological advances in the solid-state lighting industry have produced LEDs which are also suitable tools in analytical measurements. LEDs are now available which deliver 700 mW of radiometric power. While this greater light power can increase the fluorescence signal, it is not trivial to make proper use of this light. This new generation of LEDs has a large emitting area and a highly divergent beam. This presents a classic problem in optics where one must choose between either a small focused light spot, or high light collection efficiency. We have selected for light collection efficiency, which yields a light spot somewhat larger than the emitting area of the LED. This light is focused onto a flow cell. Increasing the detector cell internal diameter (i.d.) produces gains in (sensitivity)3. However, since the detector cell i.d. is smaller than the LED spot size, scattering of excitation light towards the detector remains a significant source of background signal. This can be minimized through the use of spectral filters and spatial filters in the form of pinholes. The detector produced a limit of detection (LOD) of 3 pM, which is roughly three orders of magnitude lower than other reports of LED-based fluorescence detectors. Furthermore, this LOD comes within a factor of six of much more expensive laser-based fluorescence systems. This detector has been used to monitor a separation from a gel filtration column of fluorescently labeled BSA from residual labeling reagent. The LOD of fluorescently labeled BSA is 25 pM.

  6. The Ligand Binding Region of the Sigma-1 Receptor: Studies Utilizing Photoaffinity Probes, Sphingosine and N-Alkylamines

    PubMed Central

    Ruoho, Arnold E.; Chu, Uyen B.; Ramachandran, Subramaniam; Fontanilla, Dominique; Mavlyutov, Timur; Hajipour, Abdol R.

    2015-01-01

    The sigma-1 receptor is a 26 kDa endoplasmic reticulum resident membrane protein that has been shown to have chaperone activity in addition to its promiscuous binding to pharmacological agents. Ligand binding domain(s) of the sigma-1 receptor have been identified using the E. coli expressed and purified receptor protein and novel radioiodinated azido photoaffinity probes followed by pro-teolytic and chemical cleavage strategies. The outcome of these experiments indicates that the sigma-1 receptor ligand binding regions are formed primarily by juxtaposition of its second and third hydrophobic domains, regions where the protein shares considerable homology with the fungal enzyme, sterol isomerase that is essential for the biosynthesis of ergosterol. Data indicate that these hydrophobic steroid binding domain like (SBDL) regions on the sigma-1 receptor are likely to interact selectively with N-alkyl amines such as the endogenous sphingolipids and with synthetic N-alkylamines and N-aralkylamines derivatives. A proposed model for the sigma-1 receptor is presented. PMID:22288412

  7. Gamma-Ray Flares from the Crab Nebula

    DOE PAGES

    Abdo, A. A.; Ackermann, M.; Ajello, M.; ...

    2010-01-06

    A young and energetic pulsar powers the well-known Crab Nebula. Here, we describe two separate gamma-ray (photon energy greater than 100 mega–electron volts) flares from this source detected by the Large Area Telescope on board the Fermi Gamma-ray Space Telescope. The first flare occurred in February 2009 and lasted approximately 16 days. The second flare was detected in September 2010 and lasted approximately 4 days. During these outbursts, the gamma-ray flux from the nebula increased by factors of four and six, respectively. The brevity of the flares implies that the gamma rays were emitted via synchrotron radiation from peta–electron-volt (10more » 15 electron volts) electrons in a region smaller than 1.4 × 10 -2 parsecs. In conclusion, these are the highest-energy particles that can be associated with a discrete astronomical source, and they pose challenges to particle acceleration theory.« less

  8. Gamma-Ray Pulsar Candidates for GLAST

    NASA Technical Reports Server (NTRS)

    Thompson, D. J.

    2008-01-01

    The Gamma-ray Large Area Space Telescope (GLAST) will be launched this year, and its Large Area Telescope (LAT) is expected to discover scores to hundreds of gamma-ray pulsars. This poster discusses which of the over 1700 known pulsars, mostly visible only at radio frequencies, are likely to emit greater than 100 MeV gamma rays with intensities detectable by the LAT. The main figure of merit used to select gamma-ray pulsar candidates is sqrt(E-dot)/d2, where E-dot is the energy loss due to rotational spin-down, and d is the distance to the pulsar. The figure of merit incorporates spin-down flux at earth (proportional to E-dot/d2) times efficiency, assumed proportional to l/sqrt(E-dot). A few individual objects are cited to illustrate the issues. Since large E-dot pulsars also tend to have large timing noise and occasional glitches, their ephemerides can become inaccurate in weeks to months. To detect and study the gamma-ray emission the photons must be accurately tagged with the pulse phase. With hours to days between gamma-ray photon arrival times from a pulsar and months to years of LAT exposure needed for good detections, GLAST will rely on radio and X-ray timing measurements throughout the continuous gamma-ray observations. The poster will describe efforts to coordinate pulsar timing of the candidate gamma-ray pulsars.

  9. Gamma-Ray Pulsar Candidates for GLAST

    NASA Technical Reports Server (NTRS)

    Thompson, David J.; Smith, D. A.; Dumora, D.; Guillemot, L.; Parent, D.; Reposeur, T.; Grove, E.; Romani, R. W.; Thorsett, S. E.

    2007-01-01

    The Gamma-ray Large Area Space Telescope (GLAST) will be launched less than a year from now, and its Large Area Telescope (LAT) is expected to discover scores to hundreds of gamma-ray pulsars. This poster discusses which of the over 1700 known pulsars, mostly visible only at radio Erequencies, are likely to emit greater than l00 MeV gamma rays with intensities detectable by the LAT. The main figure of merit used to select gamma-ray pulsar candidates is sqrt(E-dot)/d^2, where E-dot is the energy loss due to rotational spindown, and d is the distance to the pulsar. The figure of merit incorporates spin-down flux at earth (proportional to E-dot/d^2) times efficiency, assumed proportional to 1/sqrt(E-dot). A few individual objects are cited to illustrate the issues. Since large E-dot pulsars also tend to have large timing noise and occasional glitches, their ephemerides can become inaccurate in weeks to months. To detect and study the gamma-ray emission the photons must be accurately tagged with the pulse phase. With hours to days between gamma-ray photon arrival times from a pulsar and months to years of LAT exposure needed for good detections, GLAST will need timing measurements throughout the continuous gamma-ray observations. The poster will describe efforts to coordinate pulsar timing of the candidate gamma-ray pulsars.

  10. Streptavidin in Antibody Pretargeting. 5. Chemical Modification of Recombinant Streptavidin for Labeling with the α-Particle Emitting Radionuclides 213Bi and 211At

    PubMed Central

    Wilbur, D. Scott; Hamlin, Donald K.; Chyan, Ming-Kuan; Brechbiel, Martin W.

    2008-01-01

    We are investigating the use of recombinant streptavidin (rSAv) as a carrier molecule for the short-lived α-particle emitting radionuclides 213Bi (t1/2 = 45.6 min) and 211At (t1/2 = 7.21 h) in cancer therapy. To utilize rSAv as a carrier, it must be modified in a manner that permits rapid chelation or bonding with these short-lived radionuclides, and also modified in a manner that diminishes its natural propensity for localization in kidney. Modification for labeling with 213Bi was accomplished by conjugation of rSAv with the DTPA derivative p-isothiocyanato-benzyl-CHX-A″ (CHX-A″), 3a. Modification for direct labeling with 211At was accomplished by conjugation of rSAv with an isothiocyanatophenyl derivative of a nido-carborane (nCB), 3b, or an isothiocyanatophenyl-dPEG™/decaborate(2-) derivative, 3c. After conjugation of the chelating or bonding moiety, rSAv was further modified by reaction with an excess (50–100 equivalents) of succinic anhydride. Succinylation of the lysine amines has previously been shown to greatly diminish kidney localization. rSAv modified by conjugation with 3a and succinylated radiolabeled rapidly with 213Bi (< 5 min), providing a 72% isolated yield. 211At labeling of modified rSAv was accomplished in aqueous solution using chloramine-T as the oxidant. Astatination of rSAv conjugated with 3b and succinylated occurred very rapidly (<1 min), providing a 50% isolated radiochemical yield. Astatination of rSAv conjugated with 3c and succinylated was also very rapid (<1 min) providing 66–71% isolated radiochemical yields. Astatination of succinylated rSAv, 2a, which did not have conjugated borane cage moieties, resulted in much lower radiolabeling yield (18%). The 213Bi- or 211At-labeled modified rSAv preparations were mixed with the corresponding 125I-labeled rSAv, and dual-label in vivo distributions were obtained in athymic mice. The in vivo data show that 213Bi-labeled succinylated rSAv [213Bi]6a has tissue concentrations similar

  11. INF-gamma rearranges membrane topography of MHC-I and ICAM-1 in colon carcinoma cells.

    PubMed

    Bacsó, Zsolt; Bene, László; Damjanovich, László; Damjanovich, Sándor

    2002-01-18

    Flow-cytometric fluorescence energy transfer (FCET) measurements between fluorescently labeled cell surface MHC-I and ICAM-1 molecules indicated similar receptor patterns in the plasma membrane of interferon-gamma (INF-gamma)-treated colon carcinoma cells as those observed earlier at the surface of lymphoid cells. INF-gamma activation significantly increased the density of MHC-I and ICAM-1 proteins in the membrane. This increase in receptor density was accompanied by decreased proximity level of the homo-associated MHC-I receptors. Hetero-association of MHC-I and ICAM-1 molecules was increased by INF-gamma treatment. INF-gamma changed neither hetero- nor homo-association of transferrin receptors. By staining the sphingomyelin/cholesterol-enriched lipid microdomains with fluorescently labeled cholera toxin B subunit, we found an increase in the amount of lipid-raft associated G(M1)-gangliosides due to INF-gamma treatment. Confocal microscopic results and FCET measurements show that MHC-I and ICAM-1 are components of G(M1)-ganglioside containing lipid-rafts and also support an increase in the size of these lipid-rafts upon INF-gamma treatment.

  12. A population of gamma-ray emitting globular clusters seen with the Fermi Large Area Telescope

    DOE PAGES

    Abdo, A. A.

    2010-11-24

    Context. Globular clusters with their large populations of millisecond pulsars (MSPs) are believed to be potential emitters of high-energy gamma-ray emission. The observation of this emission provides a powerful tool to assess the millisecond pulsar population of a cluster, is essential for understanding the importance of binary systems for the evolution of globular clusters, and provides complementary insights into magnetospheric emission processes. Aims. Our goal is to constrain the millisecond pulsar populations in globular clusters from analysis of gamma-ray observations. Methods. We use 546 days of continuous sky-survey observations obtained with the Large Area Telescope aboard the Fermi Gamma-ray Spacemore » Telescope to study the gamma-ray emission towards 13 globular clusters. Results. Steady point-like high-energy gamma-ray emission has been significantly detected towards 8 globular clusters. Five of them (47 Tucanae, Omega Cen, NGC 6388, Terzan 5, and M 28) show hard spectral power indices (0.7 < Γ < 1.4) and clear evidence for an exponential cut-off in the range 1.0 - 2.6 GeV, which is the characteristic signature of magnetospheric emission from MSPs. Three of them (M 62, NGC 6440 and NGC 6652) also show hard spectral indices (1.0 < Γ < 1.7), however the presence of an exponential cut-off can not be unambiguously established. Three of them (Omega Cen, NGC 6388, NGC 6652) have no known radio or X-ray MSPs yet still exhibit MSP spectral properties. From the observed gamma-ray luminosities, we estimate the total number of MSPs that is expected to be present in these globular clusters. We show that our estimates of the MSP population correlate with the stellar encounter rate and we estimate 2600 - 4700 MSPs in Galactic globular clusters, commensurate with previous estimates. Conclusions. The observation of high-energy gamma-ray emission from globular clusters thus provides a reliable independent method to assess their millisecond pulsar populations.« less

  13. Gamma-Ray Astrophysics: New Insight Into the Universe

    NASA Technical Reports Server (NTRS)

    Fichtel, Carl E.; Trombka, Jacob I.

    1997-01-01

    During the 15 years that have passed since the first edition of this book was published, there has been a major increase in our knowledge of gamma-ray astronomy. Much of this advance arises from the extensive results that have been forthcoming from the Compton Gamma-Ray Observatory. There has been the discovery of a new class of gamma-ray objects, namely high-energy gamma- ray-emitting blazars, a special class of Active Galactic Nuclei, whose basic high-energy properties now seem to be understood. A much improved picture of our galaxy now exists in the frequency range of gamma rays. The question of whether cosmic rays are galactic or metagalactic now seems settled with certainty. Significant new information exists on the gamma-ray properties of neutron star pulsars, Seyfert galaxies, and gamma-ray bursts. Substantial new insight has been obtained on solar phenomena through gamma-ray observations. Hence, this seemed to be an appropriate time to write a new edition of this book to add the important scientific implications of these many new findings. The special importance of gamma-ray astrophysics had long been recognized by many physicists and astronomers, and theorists had pursued many aspects of the subject well before the experimental results began to become available. The slower development of the experimental side was not because of a lack of incentive, but due to the substantial experimental difficulties that had to be overcome. Thus, as the gamma-ray results became available in much greater number and detail, it was possible to build upon the theoretical work that already existed and to make substantial progress in the study of many of the phenomena involved. Consequently, a much better understanding of many of the astrophysical phenomena mentioned here and others is now possible. Our principal aims in writing this book are the same as they were for the first edition: to provide a text which describes the significance of gamma-ray astrophysics and to assemble

  14. Nova Eruptions from Radio to Gamma-raysówith AAVSO Data in the Middle (Abstract)

    NASA Astrophysics Data System (ADS)

    Mukai, K.; Kafka, S.; Chomiuk, L.; Li, R.; Finzell, T.; Linford, J.; Sokoloski, J.; Nelson, T.; Rupen, M.; Mioduszewski, A.; Weston, J.

    2018-06-01

    (Abstract only) Novae are among the longest-known class of optical transients. In recent years, V1369 Cen in the south reached magnitude 3.3 in late 2013, and had repeated (but not periodic) cycles of re-brightening. Earlier in 2013, V339 Del almost reached magnitude 4.0 during the northern summer. An expanding ball of gas, at about 10,000 K, expelled by a nuclear explosion on the surface of a white dwarf, can explain much of the visible light outputs of novae. But these spectacular visible light displays turn out to be just a small part of the show. Novae are also transient objects in the radio through gamma-raysóin addition to the warm, visible light-emitting gas, we need cold dust particles that emit in the infra-red, 10 million degree shock-heated gas that emits hard X-rays, and the exposed surface of the nuclear-burning white dwarf that emits soft X-rays. Last but not least, we need an exotic process of particle acceleration to explain the gamma-rays and some radio data.

  15. Development of the instruments for the Gamma Ray Observatory

    NASA Technical Reports Server (NTRS)

    Madden, J. J.; Kniffen, D. A.

    1986-01-01

    The Gamma Ray Observatory (GRO) is to be launched in 1988 by the STS. The GRO will feature four very large instruments: the Oriented Scintillation Spectrometer Experiment (OSSE), the Imaging Compton Telescope (COMPTEL), the Energetic Gamma Ray Experiment Telescope (EGRET) and the Burst and Transient Source Experiment (BATSE). The instruments weigh from 900-1200 kg each, and required the development of specialized lifting and dolly devices to permit their assembly, manipulation and testing. The GRO is intended a{s a tool for studying discrete celestial objects such as black holes, neutron stars and other gamma-ray emitting objects, scanning for nucleosynthesis processes, mapping the Galaxy and other, high energy galaxies in terms of gamma rays, searching for cosmological effects and observing gamma ray bursts. The instruments will be sensitive from the upper end mof X-rya wavelengths to the highest energies possible. Details of the hardware and performance specifications of each of the instruments are discussed.

  16. Some problems of the detection of the high energy gamma-radiation in space

    NASA Astrophysics Data System (ADS)

    Fradkin, M. I.; Ginzburg, V. L.; Kurnosova, L. V.; Labensky, A. G.; Razorenov, L. A.; Rusakovich, M. A.; Topchiev, N. P.; Kaplin, V. A.; Runtso, M. F.; Gorchakov, E. V.; Ignatiev, P. P.

    1995-05-01

    Diffuse gamma radiation in the Galaxy has been measured with instruments onboard the COS-B and Compton Gamma Ray Observatory (CGRO) satellites from the tens of keV up to about 30 GeV. There is no experimental data at higher energies, but this data is very important for the spectrum of primary cosmic rays and the existence of neutralinos (hypothetical supersymmetrical particles which are supposed to constitute dark matter in the Galaxy and create gamma-quanta in the process of annihilation). The GAMMA-400 collaboration is working on the design of a telescope for gamma-ray measurements in the 10-1000 GeV range. The electronics of the GAMMA-400 eliminate some hindering effects, in particular the influence of backscattered gammas emitted by the very massive calorimeter (calorimeter albedo). The GAMMA-400 project may be realized in the near future if economic conditions in Russia are favorable.

  17. Low-background gamma-ray spectrometry for the international monitoring system

    DOE PAGES

    Greenwood, L. R.; Cantaloub, M. G.; Burnett, J. L.; ...

    2016-12-28

    PNNL has developed two low-background gamma-ray spectrometers in a new shallow underground laboratory, thereby significantly improving its ability to detect low levels of gamma-ray emitting fission or activation products in airborne particulate in samples from the IMS (International Monitoring System). Furthermore, the combination of cosmic veto panels, dry nitrogen gas to reduce radon and low background shielding results in a reduction of the background count rate by about a factor of 100 compared to detectors operating above ground at our laboratory.

  18. Photoaffinity labeling of opiate receptors with /sup 3/H-etorphine: possible species differences in glycosylation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bowen, W.D.; Kooper, G.

    1986-01-01

    Opiate receptors from whole rat brain (minus cerebellum) and cow striatum were labeled irreversibly using the intrinsic photolability of /sup 3/H-etorphine. After incubation with 2 nM /sup 3/H-etorphine and centrifugal washing, membranes were irradiated with light of 254 nm. Non-specific binding was determined by carrying out incubations in presence and absence of 10 microM levallorphan. Specific binding in photolabeled membranes was 75-80%, with a photo-incorporation yield of approximately 50%. Photolabeled membranes were extracted with CHAPS/Lubrol and unbound /sup 3/H-etorphine was removed by dialysis and passage over Sephadex G-25. Solubilized proteins were then subjected to chromatography on wheat germ agglutinin, andmore » retained proteins were eluted with N-acetyl D-glucosamine (NAG). Protein profiles from rat brain and cow striatum were identical, with 89% of the total protein flowing through unretained and 11% eluted by NAG. However, the profile of radioactivity was markedly different in the two species. With rat, the specific activity (cpm/A280) was the same for flow-through and NAG-eluate. With cow, the specific activity of the NAG-eluate was 17 times greater than the flow-through. These results indicate that cow striatum and rat whole brain contain populations of opiate receptors which are glycosylated differently.« less

  19. Gamma-ray dose from an overhead plume

    DOE PAGES

    McNaughton, Michael W.; Gillis, Jessica McDonnel; Ruedig, Elizabeth; ...

    2017-05-01

    Standard plume models can underestimate the gamma-ray dose when most of the radioactive material is above the heads of the receptors. Typically, a model is used to calculate the air concentration at the height of the receptor, and the dose is calculated by multiplying the air concentration by a concentration-to-dose conversion factor. Models indicate that if the plume is emitted from a stack during stable atmospheric conditions, the lower edges of the plume may not reach the ground, in which case both the ground-level concentration and the dose are usually reported as zero. However, in such cases, the dose frommore » overhead gamma-emitting radionuclides may be substantial. Such underestimates could impact decision making in emergency situations. The Monte Carlo N-Particle code, MCNP, was used to calculate the overhead shine dose and to compare with standard plume models. At long distances and during unstable atmospheric conditions, the MCNP results agree with the standard models. As a result, at short distances, where many models calculate zero, the true dose (as modeled by MCNP) can be estimated with simple equations.« less

  20. SYSTEMATIC STUDY OF GAMMA-RAY-BRIGHT BLAZARS WITH OPTICAL POLARIZATION AND GAMMA-RAY VARIABILITY

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Itoh, Ryosuke; Fukazawa, Yasushi; Kanda, Yuka

    Blazars are highly variable active galactic nuclei that emit radiation at all wavelengths from radio to gamma rays. Polarized radiation from blazars is one key piece of evidence for synchrotron radiation at low energies, and it also varies dramatically. The polarization of blazars is of interest for understanding the origin, confinement, and propagation of jets. However, even though numerous measurements have been performed, the mechanisms behind jet creation, composition, and variability are still debated. We performed simultaneous gamma-ray and optical photopolarimetry observations of 45 blazars between 2008 July and 2014 December to investigate the mechanisms of variability and search formore » a basic relation between the several subclasses of blazars. We identify a correlation between the maximum degree of optical linear polarization and the gamma-ray luminosity or the ratio of gamma-ray to optical fluxes. Since the maximum polarization degree depends on the condition of the magnetic field (chaotic or ordered), this result implies a systematic difference in the intrinsic alignment of magnetic fields in parsec-scale relativistic jets between different types of blazars (flat-spectrum radio quasars vs. BL Lacs) and consequently between different types of radio galaxies (FR I versus FR II).« less

  1. Gamma-ray imaging system for real-time measurements in nuclear waste characterisation

    NASA Astrophysics Data System (ADS)

    Caballero, L.; Albiol Colomer, F.; Corbi Bellot, A.; Domingo-Pardo, C.; Leganés Nieto, J. L.; Agramunt Ros, J.; Contreras, P.; Monserrate, M.; Olleros Rodríguez, P.; Pérez Magán, D. L.

    2018-03-01

    A compact, portable and large field-of-view gamma camera that is able to identify, locate and quantify gamma-ray emitting radioisotopes in real-time has been developed. The device delivers spectroscopic and imaging capabilities that enable its use it in a variety of nuclear waste characterisation scenarios, such as radioactivity monitoring in nuclear power plants and more specifically for the decommissioning of nuclear facilities. The technical development of this apparatus and some examples of its application in field measurements are reported in this article. The performance of the presented gamma-camera is also benchmarked against other conventional techniques.

  2. Very-high-energy gamma rays from a distant quasar: how transparent is the universe?

    PubMed

    Albert, J; Aliu, E; Anderhub, H; Antonelli, L A; Antoranz, P; Backes, M; Baixeras, C; Barrio, J A; Bartko, H; Bastieri, D; Becker, J K; Bednarek, W; Berger, K; Bernardini, E; Bigongiari, C; Biland, A; Bock, R K; Bonnoli, G; Bordas, P; Bosch-Ramon, V; Bretz, T; Britvitch, I; Camara, M; Carmona, E; Chilingarian, A; Commichau, S; Contreras, J L; Cortina, J; Costado, M T; Covino, S; Curtef, V; Dazzi, F; De Angelis, A; De Cea Del Pozo, E; de Los Reyes, R; De Lotto, B; De Maria, M; De Sabata, F; Mendez, C Delgado; Dominguez, A; Dorner, D; Doro, M; Errando, M; Fagiolini, M; Ferenc, D; Fernández, E; Firpo, R; Fonseca, M V; Font, L; Galante, N; López, R J García; Garczarczyk, M; Gaug, M; Goebel, F; Hayashida, M; Herrero, A; Höhne, D; Hose, J; Hsu, C C; Huber, S; Jogler, T; Kneiske, T M; Kranich, D; La Barbera, A; Laille, A; Leonardo, E; Lindfors, E; Lombardi, S; Longo, F; López, M; Lorenz, E; Majumdar, P; Maneva, G; Mankuzhiyil, N; Mannheim, K; Maraschi, L; Mariotti, M; Martínez, M; Mazin, D; Meucci, M; Meyer, M; Miranda, J M; Mirzoyan, R; Mizobuchi, S; Moles, M; Moralejo, A; Nieto, D; Nilsson, K; Ninkovic, J; Otte, N; Oya, I; Panniello, M; Paoletti, R; Paredes, J M; Pasanen, M; Pascoli, D; Pauss, F; Pegna, R G; Perez-Torres, M A; Persic, M; Peruzzo, L; Piccioli, A; Prada, F; Prandini, E; Puchades, N; Raymers, A; Rhode, W; Ribó, M; Rico, J; Rissi, M; Robert, A; Rügamer, S; Saggion, A; Saito, T Y; Salvati, M; Sanchez-Conde, M; Sartori, P; Satalecka, K; Scalzotto, V; Scapin, V; Schmitt, R; Schweizer, T; Shayduk, M; Shinozaki, K; Shore, S N; Sidro, N; Sierpowska-Bartosik, A; Sillanpää, A; Sobczynska, D; Spanier, F; Stamerra, A; Stark, L S; Takalo, L; Tavecchio, F; Temnikov, P; Tescaro, D; Teshima, M; Tluczykont, M; Torres, D F; Turini, N; Vankov, H; Venturini, A; Vitale, V; Wagner, R M; Wittek, W; Zabalza, V; Zandanel, F; Zanin, R; Zapatero, J

    2008-06-27

    The atmospheric Cherenkov gamma-ray telescope MAGIC, designed for a low-energy threshold, has detected very-high-energy gamma rays from a giant flare of the distant Quasi-Stellar Radio Source (in short: radio quasar) 3C 279, at a distance of more than 5 billion light-years (a redshift of 0.536). No quasar has been observed previously in very-high-energy gamma radiation, and this is also the most distant object detected emitting gamma rays above 50 gigaelectron volts. Because high-energy gamma rays may be stopped by interacting with the diffuse background light in the universe, the observations by MAGIC imply a low amount for such light, consistent with that known from galaxy counts.

  3. Detection of 16 gamma-ray pulsars through blind frequency searches using the Fermi LAT.

    PubMed

    Abdo, A A; Ackermann, M; Ajello, M; Anderson, B; Atwood, W B; Axelsson, M; Baldini, L; Ballet, J; Barbiellini, G; Baring, M G; Bastieri, D; Baughman, B M; Bechtol, K; Bellazzini, R; Berenji, B; Bignami, G F; Blandford, R D; Bloom, E D; Bonamente, E; Borgland, A W; Bregeon, J; Brez, A; Brigida, M; Bruel, P; Burnett, T H; Caliandro, G A; Cameron, R A; Caraveo, P A; Casandjian, J M; Cecchi, C; Celik, O; Chekhtman, A; Cheung, C C; Chiang, J; Ciprini, S; Claus, R; Cohen-Tanugi, J; Conrad, J; Cutini, S; Dermer, C D; de Angelis, A; de Luca, A; de Palma, F; Digel, S W; Dormody, M; do Couto e Silva, E; Drell, P S; Dubois, R; Dumora, D; Farnier, C; Favuzzi, C; Fegan, S J; Fukazawa, Y; Funk, S; Fusco, P; Gargano, F; Gasparrini, D; Gehrels, N; Germani, S; Giebels, B; Giglietto, N; Giommi, P; Giordano, F; Glanzman, T; Godfrey, G; Grenier, I A; Grondin, M-H; Grove, J E; Guillemot, L; Guiriec, S; Gwon, C; Hanabata, Y; Harding, A K; Hayashida, M; Hays, E; Hughes, R E; Jóhannesson, G; Johnson, R P; Johnson, T J; Johnson, W N; Kamae, T; Katagiri, H; Kataoka, J; Kawai, N; Kerr, M; Knödlseder, J; Kocian, M L; Kuss, M; Lande, J; Latronico, L; Lemoine-Goumard, M; Longo, F; Loparco, F; Lott, B; Lovellette, M N; Lubrano, P; Madejski, G M; Makeev, A; Marelli, M; Mazziotta, M N; McConville, W; McEnery, J E; Meurer, C; Michelson, P F; Mitthumsiri, W; Mizuno, T; Monte, C; Monzani, M E; Morselli, A; Moskalenko, I V; Murgia, S; Nolan, P L; Norris, J P; Nuss, E; Ohsugi, T; Omodei, N; Orlando, E; Ormes, J F; Paneque, D; Parent, D; Pelassa, V; Pepe, M; Pesce-Rollins, M; Pierbattista, M; Piron, F; Porter, T A; Primack, J R; Rainò, S; Rando, R; Ray, P S; Razzano, M; Rea, N; Reimer, A; Reimer, O; Reposeur, T; Ritz, S; Rochester, L S; Rodriguez, A Y; Romani, R W; Ryde, F; Sadrozinski, H F-W; Sanchez, D; Sander, A; Saz Parkinson, P M; Scargle, J D; Sgrò, C; Siskind, E J; Smith, D A; Smith, P D; Spandre, G; Spinelli, P; Starck, J-L; Strickman, M S; Suson, D J; Tajima, H; Takahashi, H; Takahashi, T; Tanaka, T; Thayer, J G; Thompson, D J; Tibaldo, L; Tibolla, O; Torres, D F; Tosti, G; Tramacere, A; Uchiyama, Y; Usher, T L; Van Etten, A; Vasileiou, V; Vilchez, N; Vitale, V; Waite, A P; Wang, P; Watters, K; Winer, B L; Wolff, M T; Wood, K S; Ylinen, T; Ziegler, M

    2009-08-14

    Pulsars are rapidly rotating, highly magnetized neutron stars emitting radiation across the electromagnetic spectrum. Although there are more than 1800 known radio pulsars, until recently only seven were observed to pulse in gamma rays, and these were all discovered at other wavelengths. The Fermi Large Area Telescope (LAT) makes it possible to pinpoint neutron stars through their gamma-ray pulsations. We report the detection of 16 gamma-ray pulsars in blind frequency searches using the LAT. Most of these pulsars are coincident with previously unidentified gamma-ray sources, and many are associated with supernova remnants. Direct detection of gamma-ray pulsars enables studies of emission mechanisms, population statistics, and the energetics of pulsar wind nebulae and supernova remnants.

  4. Picosecond Pulse Recirculation for High Average Brightness Thomson Scattering-based Gamma-ray Sources

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Semenov, V. A.

    2009-06-12

    Pulse recirculation has been successfully demonstrated with the interaction laser system of LLNL's Thomson-Radiated Extreme X-ray (T-REX) source. The recirculation increased twenty-eight times the intensity of the light coming out of the laser system, demonstrating the capability of increasing the gamma-ray flux emitted by T-REX. The technical approach demonstrated could conceivably increase the average gamma-ray flux output by up to a hundred times.

  5. Measurements of the gamma-quanta angular distributions emitted from neutron inelastic scattering on 28Si

    NASA Astrophysics Data System (ADS)

    Fedorov, N. A.; Grozdanov, D. N.; Bystritskiy, V. M.; Kopach, Yu. N.; Ruskov, I. N.; Skoy, V. R.; Tretyakova, T. Yu.; Zamyatin, N. I.; Wang, D.; Aliev, F. A.; Hramco, C.; Gandhi, A.; Kumar, A.; Dabylova, S.; Bogolubov, E. P.; Barmakov, Yu. N.

    2018-04-01

    The characteristic gamma radiation from the interaction of 14.1 MeV neutrons with a natural silicon sample is investigated with Tagged Neutron Method (TNM). The anisotropy of gamma-ray emission of 1.779 MeV was measured at 11 azimuth angles with a step of ∠15°. The present results are in good agreement with some recent experimental data.

  6. Assessment of gamma-emitting radionuclides in sediment cores from the Gulf of Aqaba, Red Sea.

    PubMed

    Ababneh, Zaid Q; Al-Omari, Husam; Rasheed, Mohamad; Al-Najjar, Tariq; Ababneh, Anas M

    2010-10-01

    The Gulf of Aqaba is the only seaport in Jordan which currently has intense activities such as industrial development, phosphate ore exportation, oil importation, shipping, commercial and sport fishing. Most of these activities, especially the phosphate ore exportation, could cause serious radiological effects to the marine environment. Thus, it is essential to investigate the level of the radioactivity concentrations to establish a baseline database, which is not available yet in the Gulf of Aqaba. Radioactivity concentrations of gamma-emitting radionuclides in core and beach sediments of the Gulf of Aqaba were investigated. Core sediments were collected from five representative locations for three different water column depths (5, 15 and 35 m). The results showed that the activity concentrations of 238U, 235U and 226Ra for both seafloor and beach sediments from the phosphate loading berth (PLB) location to be higher than those from other investigated locations and more than twice as high as the worldwide average; the 238U activity concentration was found to vary from 57 to 677 Bq kg(-1). The results also showed that there is little variation of radioactivity concentrations within the core length of 15 cm. The calculated mean values of the radium equivalent activity Ra(eq), the external hazard index, H(ex), the absorbed dose rate and the annual effective dose for the beach sediment in PLB location were 626 Bq kg(-1), 1.69, 263 nGy h(-1) and 614 µSv y(-1), respectively. These values are much higher than the recommended limits that impose potential health risks to the workers in this location. As for other studied locations, the corresponding values were far below the maximum recommended limit and lies within the worldwide range.

  7. Early optical polarization of a gamma-ray burst afterglow.

    PubMed

    Mundell, Carole G; Steele, Iain A; Smith, Robert J; Kobayashi, Shiho; Melandri, Andrea; Guidorzi, Cristiano; Gomboc, Andreja; Mottram, Chris J; Clarke, David; Monfardini, Alessandro; Carter, David; Bersier, David

    2007-03-30

    We report the optical polarization of a gamma-ray burst (GRB) afterglow, obtained 203 seconds after the initial burst of gamma-rays from GRB 060418, using a ring polarimeter on the robotic Liverpool Telescope. Our robust (2sigma) upper limit on the percentage of polarization, less than 8%, coincides with the fireball deceleration time at the onset of the afterglow. The combination of the rate of decay of the optical brightness and the low polarization at this critical time constrains standard models of GRB ejecta, ruling out the presence of a large-scale ordered magnetic field in the emitting region.

  8. Using gamma-ray emission to measure areal density of inertial confinement fusion capsulesa)

    NASA Astrophysics Data System (ADS)

    Hoffman, N. M.; Wilson, D. C.; Herrmann, H. W.; Young, C. S.

    2010-10-01

    Fusion neutrons streaming from a burning inertial confinement fusion capsule generate gamma rays via inelastic nuclear scattering in the ablator of the capsule. The intensity of gamma-ray emission is proportional to the product of the ablator areal density (ρR) and the yield of fusion neutrons, so by detecting the gamma rays we can infer the ablator areal density, provided we also have a measurement of the capsule's total neutron yield. In plastic-shell capsules, for example, C12 nuclei emit gamma rays at 4.44 MeV after excitation by 14.1 MeV neutrons from D+T fusion. These gamma rays can be measured by a new gamma-ray detector under development. Analysis of predicted signals is in progress, with results to date indicating that the method promises to be useful for diagnosing imploded capsules.

  9. Photoaffinity labelling of cyclic GMP-inhibited phosphodiesterase (PDE III) in human and rat platelets and rat tissues: effects of phosphodiesterase inhibitors.

    PubMed

    Tang, K M; Jang, E K; Haslam, R J

    1994-06-15

    Ultraviolet irradiation of human platelet cytosol in the presence of 32P-labelled cyclic GMP (cGMP) can specifically label 110, 80, 55, 49 and 38 kDa proteins; the 110 kDa species is the subunit of cGMP-inhibited phosphodiesterase (PDE III) and the 80 kDa species that of cGMP-dependent protein kinase (Tang et al., 1993, Biochem. J. 294, 329). We have now shown that although photolabelling of platelet PDE III was inhibited by unlabelled cGMP, 8-bromo-cGMP and cyclic AMP (cAMP), it was not affected by phosphorothioate analogues of these cyclic nucleotides. Specific concentration-dependent inhibitions of the photolabelling of PDE III were observed with the following PDE inhibitors: trequinsin (IC50 = 13 +/- 2 nM), lixazinone (IC50 = 22 +/- 4 nM), milrinone (IC50 = 56 +/- 12 nM), cilostamide (IC50 = 70 +/- 9 nM), siguazodan (IC50 = 117 +/- 29 nM) and 3-isobutyl 1-methylxanthine (IBMX) (IC50 = 3950 +/- 22 nM). Thus, measurements of the inhibitory effects of compounds on the photolabelling of platelet PDE III provide a simple quantitative means of investigating their actions at a molecular level that avoids the need to purify the enzyme. Photolabelling of rat platelet lysate or rat heart homogenate by [32P]cGMP showed that the 110 kDa PDE III present in human material was replaced by a 115 kDa protein, labelling of which was also blocked by PDE III inhibitors. Heart and other rat tissues contained much less of this putative 115 kDa PDE III than rat platelets. In contrast, the 80 kDa protein was labelled much less in platelets than in many other rat tissue homogenates (e.g., heart, aorta, uterus and lung). Thus, comparison of the relative amounts of specific photolabelled proteins in different cells may provide an indication of different patterns of cyclic nucleotide action. We compared the abilities of phosphodiesterase inhibitors to block the photolabelling of PDE III in human platelet cytosol and to increase the iloprost-stimulated accumulation of cAMP in intact

  10. Reducing Uncertainties in the Production of the Gamma-emitting Nuclei 26Al, 44Ti, and 60Fe in Core-collapse Supernovae by Using Effective Helium Burning Rates

    NASA Astrophysics Data System (ADS)

    Austin, Sam M.; West, Christopher; Heger, Alexander

    2017-04-01

    We have used effective reaction rates (ERRs) for the helium burning reactions to predict the yield of the gamma-emitting nuclei 26Al, 44Ti, and 60Fe in core-collapse supernovae (SNe). The variations in the predicted yields for values of the reaction rates allowed by the ERR are much smaller than obtained previously, and smaller than other uncertainties. A “filter” for SN nucleosynthesis yields based on pre-SN structure was used to estimate the effect of failed SNe on the initial mass function averaged yields; this substantially reduced the yields of all these isotopes, but the predicted yield ratio 60Fe/26Al was little affected. The robustness of this ratio is promising for comparison with data, but it is larger than observed in nature; possible causes for this discrepancy are discussed.

  11. SBS 0846+513: a New Gamma-ray Emitting Narrow-line Seyfert 1 Galaxy

    NASA Technical Reports Server (NTRS)

    D'Ammando, F.; Orienti, M.; Finke, J.; Raiteri, C. M.; Angelakis, E.; Fuhrmann, L.; Giroletti, M.; Hovatta, T.; Max-Moerbeck, W.; Perkins, J. S.; hide

    2012-01-01

    We report Fermi-LAT observations of the radio-loud AGN SBS 0846+513 (z=0.5835), optically classified as a Narrow-Line Seyfert 1 galaxy, together with new and archival radio-to-X-ray data. The source was not active at ?-ray energies during the first two years of Fermi operation. A significant increase in activity was observed during 2010 October-2011 August. In particular a strong gamma-ray flare was observed in 2011 June reaching an isotropic ?-ray luminosity (0.1-300 GeV) of 1.0×10(sup 48) erg s(sup -1), comparable to that of the brightest flat spectrum radio quasars, and showing spectral evolution in gamma rays. An apparent superluminal velocity of (8.2+/-1.5)c in the jet was inferred from 2011-2012 VLBA images, suggesting the presence of a highly relativistic jet. Both the power released by this object during the flaring activity and the apparent superluminal velocity are strong indications of the presence of a relativistic jet as powerful as those of blazars. In addition, variability and spectral properties in radio and gamma-ray bands indicate blazar-like behaviour, suggesting that, except for some distinct optical characteristics, SBS 0846+513 could be considered as a young blazar at the low end of the blazar's black hole mass distribution.

  12. Observations of Soft Gamma Repeaters

    NASA Technical Reports Server (NTRS)

    Kouveliotou, Chryssa

    2004-01-01

    Magnetars (Soft Gamma Repeaters and Anomalous X-ray Pulsars) are a subclass of neutron stars characterized by their recurrent X-ray bursts. While in an active (bursting) state (lasting anywhere between days and years), they are emit&ng hundreds of predominantly soft (kT=30 kev), short (0.1-100 ms long) events. Their quiescent source x-ray light ewes exhibit puhlions rotational period rate changes (spin-down) indicate that their magnetic fields are extremely high, of the order of 10^14- 10^l5 G. Such high B-field objects, dubbed "magnetars", had been predicted to exist in 1992, but the first concrete observational evidence were obtained in 1998 for two of these sources. I will discuss here the history of Soft Gamma Repeaters, and their spectral, timing and flux characteristics both in the persistent and their burst emission.

  13. Separation of gamma-ray and neutron events with CsI(Tl) pulse shape analysis

    NASA Astrophysics Data System (ADS)

    Ashida, Y.; Nagata, H.; Koshio, Y.; Nakaya, T.; Wendell, R.

    2018-04-01

    Fast neutrons are a large background to measurements of gamma-rays emitted from excited nuclei, such that detectors that can efficiently distinguish between the two are essential. In this paper we describe the separation of gamma-rays from neutrons with the pulse shape information of the CsI(Tl) scintillator, using a fast neutron beam and several gamma-ray sources. We find that a figure of merit optimized for this separation takes on large and stable values (nearly 4) between 5 and 10 MeV of electron equivalent deposited energy, the region of most interest to the study of nuclear de-excitation gamma-rays. Accordingly, this work demonstrates the ability of CsI(Tl) scintillators to reject neutron backgrounds to gamma-ray measurements at these energies.

  14. Detection of 16 Gamma-Ray Pulsars Through Blind Frequency Searches Using the Fermi LAT

    DOE PAGES

    Abdo, A. A.; Ackermann, M.; Ajello, M.; ...

    2009-07-02

    Pulsars are rapidly rotating, highly magnetized neutron stars emitting radiation across the electromagnetic spectrum. Although there are more than 1800 known radio pulsars, until recently only seven were observed to pulse in gamma rays, and these were all discovered at other wavelengths. The Fermi Large Area Telescope (LAT) makes it possible to pinpoint neutron stars through their gamma-ray pulsations. In this paper, we report the detection of 16 gamma-ray pulsars in blind frequency searches using the LAT. Most of these pulsars are coincident with previously unidentified gamma-ray sources, and many are associated with supernova remnants. Finally, direct detection of gamma-raymore » pulsars enables studies of emission mechanisms, population statistics, and the energetics of pulsar wind nebulae and supernova remnants.« less

  15. Found: A Galaxy's Missing Gamma Rays

    NASA Astrophysics Data System (ADS)

    Kohler, Susanna

    2016-04-01

    Recent reanalysis of data from the Fermi Gamma-ray Space Telescope has resulted in the first detection of high-energy gamma rays emitted from a nearby galaxy. This discovery reveals more about how supernovae interact with their environments.Colliding Supernova RemnantAfter a stellar explosion, the supernovas ejecta expand, eventually encountering the ambient interstellar medium. According to models, this generates a strong shock, and a fraction of the kinetic energy of the ejecta is transferred into cosmic rays high-energy radiation composed primarily of protons and atomic nuclei. Much is still unknown about this process, however. One open question is: what fraction of the supernovas explosion power goes into accelerating these cosmic rays?In theory, one way to answer this is by looking for gamma rays. In a starburst galaxy, the collision of the supernova-accelerated cosmic rays with the dense interstellar medium is predicted to produce high-energy gamma rays. That radiation should then escape the galaxy and be visible to us.Pass 8 to the RescueObservational tests of this model, however, have beenstumped by Arp 220. This nearby ultraluminous infrared galaxy is the product of a galaxy merger ~700 million years ago that fueled a frenzy of starbirth. Due to its dusty interior and extreme levels of star formation, Arp 220 has long been predicted to emit the gamma rays produced by supernova-accelerated cosmic rays. But though weve looked, gamma-ray emission has never been detected from this galaxy until now.In a recent study, a team of scientists led by Fang-Kun Peng (Nanjing University) reprocessed 7.5 years of Fermi observations using the new Pass 8 analysis software. The resulting increase in resolution revealed the first detection of GeV emission from Arp 220!Acceleration EfficiencyGamma-ray luminosity vs. total infrared luminosity for LAT-detected star-forming galaxies and Seyferts. Arp 220s luminosities are consistent with the scaling relation. [Peng et al. 2016

  16. The Use of the BAT Instrument on SWIFT for the Detection of Prompt Gamma-Ray Emission from Novae

    NASA Technical Reports Server (NTRS)

    Skinner, Gerry; Senziani, Fabio; Jean, Pierre; Hernanz, Margarita

    2007-01-01

    Gamma-rays are expected to be emitted during and immediately following a nova explosion due to the annihilation of positrons emitted by freshly produced short-lived radioactive isotopes. The expected gammaray emission is relatively short-lived and as nova explosions are unpredictable, the best chance of detecting the gamma-rays is with n wide field instrument. At the time when the flux is expected to rcach its peak, most of the gamma-ray production is at depths such that the photons suffer several Compton scatterings before escaping, degrading their energy down to the hard X-ray band (10s of keV). SWIFT/BAT is a very wide field coded mask instrument working in the energy band 14-190 keV and so is very well suited to the search for such gamma-rays. A retrospective search is being made in the BAT data for evidence for gamma-ray emission from the direction of novae at around the time of their explosion. So far the only positive detection is of RS Ophiuchi and in this case the emission is probably due to shock heating.

  17. A giant gamma-ray flare from the magnetar SGR 1806-20.

    PubMed

    Palmer, D M; Barthelmy, S; Gehrels, N; Kippen, R M; Cayton, T; Kouveliotou, C; Eichler, D; Wijers, R A M J; Woods, P M; Granot, J; Lyubarsky, Y E; Ramirez-Ruiz, E; Barbier, L; Chester, M; Cummings, J; Fenimore, E E; Finger, M H; Gaensler, B M; Hullinger, D; Krimm, H; Markwardt, C B; Nousek, J A; Parsons, A; Patel, S; Sakamoto, T; Sato, G; Suzuki, M; Tueller, J

    2005-04-28

    Two classes of rotating neutron stars-soft gamma-ray repeaters (SGRs) and anomalous X-ray pulsars-are magnetars, whose X-ray emission is powered by a very strong magnetic field (B approximately 10(15) G). SGRs occasionally become 'active', producing many short X-ray bursts. Extremely rarely, an SGR emits a giant flare with a total energy about a thousand times higher than in a typical burst. Here we report that SGR 1806-20 emitted a giant flare on 27 December 2004. The total (isotropic) flare energy is 2 x 10(46) erg, which is about a hundred times higher than the other two previously observed giant flares. The energy release probably occurred during a catastrophic reconfiguration of the neutron star's magnetic field. If the event had occurred at a larger distance, but within 40 megaparsecs, it would have resembled a short, hard gamma-ray burst, suggesting that flares from extragalactic SGRs may form a subclass of such bursts.

  18. Development of an aptamer beacon for detection of interferon-gamma.

    PubMed

    Tuleuova, Nazgul; Jones, Caroline N; Yan, Jun; Ramanculov, Erlan; Yokobayashi, Yohei; Revzin, Alexander

    2010-03-01

    Traditional antibody-based affinity sensing strategies employ multiple reagents and washing steps and are unsuitable for real-time detection of analyte binding. Aptamers, on the other hand, may be designed to monitor binding events directly, in real-time, without the need for secondary labels. The goal of the present study was to design an aptamer beacon for fluorescence resonance energy transfer (FRET)-based detection of interferon-gamma (IFN-gamma)--an important inflammatory cytokine. Variants of DNA aptamer modified with biotin moieties and spacers were immobilized on avidin-coated surfaces and characterized by surface plasmon resonance (SPR). The SPR studies showed that immobilization of aptamer via the 3' end resulted in the best binding IFN-gamma (K(d) = 3.44 nM). This optimal aptamer variant was then used to construct a beacon by hybridizing fluorophore-labeled aptamer with an antisense oligonucleotide strand carrying a quencher. SPR studies revealed that IFN-gamma binding with an aptamer beacon occurred within 15 min of analyte introduction--suggesting dynamic replacement of the quencher-complementary strand by IFN-gamma molecules. To further highlight biosensing applications, aptamer beacon molecules were immobilized inside microfluidic channels and challenged with varying concentration of analyte. Fluorescence microscopy revealed low fluorescence in the absence of analyte and high fluorescence after introduction of IFN-gamma. Importantly, unlike traditional antibody-based immunoassays, the signal was observed directly upon binding of analyte without the need for multiple washing steps. The surface immobilized aptamer beacon had a linear range from 5 to 100 nM and a lower limit of detection of 5 nM IFN-gamma. In conclusion, we designed a FRET-based aptamer beacon for monitoring of an inflammatory cytokine-IFN-gamma. In the future, this biosensing strategy will be employed to monitor dynamics of cytokine production by the immune cells.

  19. Supervised Gamma Process Poisson Factorization

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Anderson, Dylan Zachary

    This thesis develops the supervised gamma process Poisson factorization (S- GPPF) framework, a novel supervised topic model for joint modeling of count matrices and document labels. S-GPPF is fully generative and nonparametric: document labels and count matrices are modeled under a uni ed probabilistic framework and the number of latent topics is controlled automatically via a gamma process prior. The framework provides for multi-class classification of documents using a generative max-margin classifier. Several recent data augmentation techniques are leveraged to provide for exact inference using a Gibbs sampling scheme. The first portion of this thesis reviews supervised topic modeling andmore » several key mathematical devices used in the formulation of S-GPPF. The thesis then introduces the S-GPPF generative model and derives the conditional posterior distributions of the latent variables for posterior inference via Gibbs sampling. The S-GPPF is shown to exhibit state-of-the-art performance for joint topic modeling and document classification on a dataset of conference abstracts, beating out competing supervised topic models. The unique properties of S-GPPF along with its competitive performance make it a novel contribution to supervised topic modeling.« less

  20. GeV-gamma-ray emission regions

    NASA Image and Video Library

    2017-12-08

    NASA's Fermi Closes on Source of Cosmic Rays New images from NASA's Fermi Gamma-ray Space Telescope show where supernova remnants emit radiation a billion times more energetic than visible light. The images bring astronomers a step closer to understanding the source of some of the universe's most energetic particles -- cosmic rays. Fermi mapped GeV-gamma-ray emission regions (magenta) in the W44 supernova remnant. The features clearly align with filaments detectable in other wavelengths. This composite merges X-rays (blue) from the Germany-led ROSAT mission, infrared (red) from NASA's Spitzer Space Telescope, and radio (orange) from the Very Large Array near Socorro, N.M. Credit: NASA/DOE/Fermi LAT Collaboration, ROSAT, JPL-Caltech, and NRAO/AUI For more information: www.nasa.gov/mission_pages/GLAST/news/cosmic-rays-source....

  1. Colloid labelled with radionuclide and method

    DOEpatents

    Atcher, Robert W.; Hines, John J.

    1990-01-01

    A ferric hydroxide colloid having an alpha-emitting radionuclide essentially on the outer surfaces and a method of forming same. The method includes oxidizing a ferrous hydroxide to ferric hydroxide in the presence of a preselected radionuclide to form a colloid having the radionuclide on the outer surface thereof, and thereafter washing the colloid, and suspending the washed colloid in a suitable solution. The labelled colloid is useful in cancer therapy and for the treatment of inflamed joints.

  2. Method and system for determining depth distribution of radiation-emitting material located in a source medium and radiation detector system for use therein

    DOEpatents

    Benke, Roland R.; Kearfott, Kimberlee J.; McGregor, Douglas S.

    2003-03-04

    A method, system and a radiation detector system for use therein are provided for determining the depth distribution of radiation-emitting material distributed in a source medium, such as a contaminated field, without the need to take samples, such as extensive soil samples, to determine the depth distribution. The system includes a portable detector assembly with an x-ray or gamma-ray detector having a detector axis for detecting the emitted radiation. The radiation may be naturally-emitted by the material, such as gamma-ray-emitting radionuclides, or emitted when the material is struck by other radiation. The assembly also includes a hollow collimator in which the detector is positioned. The collimator causes the emitted radiation to bend toward the detector as rays parallel to the detector axis of the detector. The collimator may be a hollow cylinder positioned so that its central axis is perpendicular to the upper surface of the large area source when positioned thereon. The collimator allows the detector to angularly sample the emitted radiation over many ranges of polar angles. This is done by forming the collimator as a single adjustable collimator or a set of collimator pieces having various possible configurations when connected together. In any one configuration, the collimator allows the detector to detect only the radiation emitted from a selected range of polar angles measured from the detector axis. Adjustment of the collimator or the detector therein enables the detector to detect radiation emitted from a different range of polar angles. The system further includes a signal processor for processing the signals from the detector wherein signals obtained from different ranges of polar angles are processed together to obtain a reconstruction of the radiation-emitting material as a function of depth, assuming, but not limited to, a spatially-uniform depth distribution of the material within each layer. The detector system includes detectors having

  3. N-(3-azidophenyl)-N-methyl-N'-([4-1H]- and [4-3H]-1-naphthyl)guanidine. A potent and selective ligand designed as a photoaffinity label for the phencyclidine site of the N-methyl-D-aspartate receptor.

    PubMed

    Gee, K R; Durant, G J; Holmes, D L; Magar, S S; Weber, E; Wong, S T; Keana, J F

    1993-01-01

    A novel radiolabeled photoaffinity ligand has been synthesized for the phencyclidine (PCP) site of the N-methyl-D-aspartate (NMDA) receptor. N-(3-Azidophenyl)-N-methyl-N'-([4-3H]-1-naphthyl)guanidine (13) was prepared with a specific activity of 25 Ci/mmol by diazotization of N-(3-aminophenyl)-N-methyl-N'-([4-3H]-1-naphthyl)guanidine (12) followed by treatment with sodium azide. Guanidine 12 was obtained by catalytic tritiation of N-(4-bromo-1-naphthyl)-N'-methyl-N'-(3-nitrophenyl)guanidine (11). The nontritiated analog 5 of 13 was prepared beginning with N-methyl-N'-1-naphthyl-N-(3-nitrophenyl)guanidine (9). The guanidines 9 and 11 were prepared in moderate yield by the aluminum chloride-catalyzed reaction of N-methyl-3-nitroaniline hydrochloride with 1-naphthylcyanamide and 4-bromo-1-naphthylcyanamide, respectively. Azide 5 showed high selectivity and affinity (IC50 = 100 nM vs [3H]MK801; 3000 nM vs [3H]ditolylguanidine) for the PCP site of the NMDA receptor in guinea pig brain homogenate. Photolabeling experiments with 13, however, failed to radiolabel a significant amount of receptor polypeptide.

  4. Transmembrane topology, subcellular distribution and turnover of the gamma-aminobutyric acid/benzodizaepine receptor in chick brain cell cultures

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Czajkowski, C.M.

    1987-01-01

    Experiments were performed utilizing trypsinization of the GABA/BZD-R in intact cells to determine (1) the subcellular distribution of membrane-associated GABA/BZD-Rs and (2) aspects of the transmembrane topology of the BZD-R. Additionally, R07-0213, a positively charged benzodiazepine, was used to distinguish between cell surface and intracellular BZD-Rs. Following trypsin treatment of intact cells a cleaved receptor fragment of M{sub r} = 24,000 (xRF24) is generated. It remains anchored in the plasma membrane and not only retains the ability to bind ({sup 3}H)flunitrazepan reversibly and irreversibly but also retains the ability to be modulated by GABA. xRF24 is not observed following trypsinizationmore » of saponin-treated cells or cell homogenates, indicating that it has a cytoplasmic domain as well as a cell surface domain, as expected for a transmembrane fragment of the BZD-R. By utilizing ({sup 3}H)flunitrazepam as an irreversible photoaffinity label, BZD-R turnover was also investigated.« less

  5. Identification of an intracellular protein that specifically interacts with photoaffinity-labeled oncogenic p21 protein.

    PubMed

    Lee, G; Ronai, Z A; Pincus, M R; Brandt-Rauf, P W; Murphy, R B; Delohery, T M; Nishimura, S; Yamaizumi, Z; Weinstein, I B

    1989-11-01

    An oncogenic 21-kDa (p21) protein (Harvey RAS protein with Val-12) has been covalently modified with a functional reagent that contains a photoactivatable aromatic azide group. This modified p21 protein has been introduced quantitatively into NIH 3T3 cells using an erythrocyte-mediated fusion technique. The introduced p21 protein was capable of inducing enhanced pinocytosis and DNA synthesis in the recipient cells. To identify the putative intracellular protein(s) that specifically interact with the modified p21 protein, the cells were pulsed with [35S]methionine at selected times after fusion and then UV-irradiated to activate the azide group. The resulting nitrene covalently binds to amino acid residues in adjacent proteins, thus linking the p21 protein to these proteins. The cells were then lysed, and the lysate was immunoprecipitated with the anti-p21 monoclonal antibody Y13-259. The immunoprecipitate was analyzed by SDS/PAGE to identify p21-protein complexes. By using this technique, we found that three protein complexes of 51, 64, and 82 kDa were labeled specifically and reproducibly. The most prominent band is the 64-kDa protein complex that shows a time-dependent rise and fall, peaking within a 5-hr period after introduction of the p21 protein into the cells. These studies provide evidence that in vitro the p21 protein becomes associated with a protein whose mass is about 43 kDa. We suggest that the formation of this complex may play a role in mediating early events involved with cell transformation induced by RAS oncogenes.

  6. Identification of an intracellular protein that specifically interacts with photoaffinity-labeled oncogenic p21 protein.

    PubMed Central

    Lee, G; Ronai, Z A; Pincus, M R; Brandt-Rauf, P W; Murphy, R B; Delohery, T M; Nishimura, S; Yamaizumi, Z; Weinstein, I B

    1989-01-01

    An oncogenic 21-kDa (p21) protein (Harvey RAS protein with Val-12) has been covalently modified with a functional reagent that contains a photoactivatable aromatic azide group. This modified p21 protein has been introduced quantitatively into NIH 3T3 cells using an erythrocyte-mediated fusion technique. The introduced p21 protein was capable of inducing enhanced pinocytosis and DNA synthesis in the recipient cells. To identify the putative intracellular protein(s) that specifically interact with the modified p21 protein, the cells were pulsed with [35S]methionine at selected times after fusion and then UV-irradiated to activate the azide group. The resulting nitrene covalently binds to amino acid residues in adjacent proteins, thus linking the p21 protein to these proteins. The cells were then lysed, and the lysate was immunoprecipitated with the anti-p21 monoclonal antibody Y13-259. The immunoprecipitate was analyzed by SDS/PAGE to identify p21-protein complexes. By using this technique, we found that three protein complexes of 51, 64, and 82 kDa were labeled specifically and reproducibly. The most prominent band is the 64-kDa protein complex that shows a time-dependent rise and fall, peaking within a 5-hr period after introduction of the p21 protein into the cells. These studies provide evidence that in vitro the p21 protein becomes associated with a protein whose mass is about 43 kDa. We suggest that the formation of this complex may play a role in mediating early events involved with cell transformation induced by RAS oncogenes. Images PMID:2682656

  7. Neutron-driven gamma-ray laser

    DOEpatents

    Bowman, Charles D.

    1990-01-01

    A lasing cylinder emits laser radiation at a gamma-ray wavelength of 0.87 .ANG. when subjected to an intense neutron flux of about 400 eV neutrons. A 250 .ANG. thick layer of Be is provided between two layers of 100 .ANG. thick layer of .sup.57 Co and these layers are supported on a foil substrate. The coated foil is coiled to form the lasing cylinder. Under the neutron flux .sup.57 Co becomes .sup.58 Co by neutron absorption. The .sup.58 Co then decays to .sup.57 Fe by 1.6 MeV proton emission. .sup.57 Fe then transitions by mesne decay to a population inversion for lasing action at 14.4 keV. Recoil from the proton emission separates the .sup.57 Fe from the .sup.57 Co and into the Be, where Mossbauer emission occurs at a gamma-ray wavelength.

  8. Precision Gamma-Ray Branching Ratios for Long-Lived Radioactive Nuclei

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tonchev, Anton

    Many properties of the high-energy-density environments in nuclear weapons tests, advanced laser-fusion experiments, the interior of stars, and other astrophysical bodies must be inferred from the resulting long-lived radioactive nuclei that are produced. These radioactive nuclei are most easily and sensitively identified by studying the characteristic gamma rays emitted during decay. Measuring a number of decays via detection of the characteristic gamma-rays emitted during the gamma-decay (the gamma-ray branching ratio) of the long-lived fission products is one of the most straightforward and reliable ways to determine the number of fissions that occurred in a nuclear weapon test. The fission productsmore » 147Nd, 144Ce, 156Eu, and certain other long-lived isotopes play a crucial role in science-based stockpile stewardship, however, the large uncertainties (about 8%) on the branching ratios measured for these isotopes are currently limiting the usefulness of the existing data [1,2]. We performed highly accurate gamma-ray branching-ratio measurements for a group of high-atomic-number rare earth isotopes to greatly improve the precision and reliability with which the fission yield and reaction products in high-energy-density environments can be determined. We have developed techniques that take advantage of new radioactive-beam facilities, such as DOE's CARIBU located at Argonne National Laboratory, to produce radioactive samples and perform decay spectroscopy measurements. The absolute gamma-ray branching ratios for 147Nd and 144Ce are reduced <2% precision. In addition, high-energy monoenergetic neutron beams from the FN Tandem accelerator in TUNL at Duke University was used to produce 167Tm using the 169Tm(n,3n) reaction. Fourtime improved branching ratio of 167Tm is used now to measure reaction-in-flight (RIF) neutrons from a burning DT capsule at NIF [10]. This represents the first measurement of RIF neutrons in any laboratory fusion system, and the magnitude

  9. Colloid labelled with radionuclide and method

    DOEpatents

    Atcher, R.W.; Hines, J.J.

    1990-11-13

    A ferric hydroxide colloid having an alpha-emitting radionuclide essentially on the outer surfaces and a method of forming same. The method includes oxidizing a ferrous hydroxide to ferric hydroxide in the presence of a preselected radionuclide to form a colloid having the radionuclide on the outer surface thereof, and thereafter washing the colloid, and suspending the washed colloid in a suitable solution. The labelled colloid is useful in cancer therapy and for the treatment of inflamed joints. No Drawings

  10. Limits to the Fraction of High-energy Photon Emitting Gamma-Ray Bursts

    NASA Astrophysics Data System (ADS)

    Akerlof, Carl W.; Zheng, WeiKang

    2013-02-01

    After almost four years of operation, the two instruments on board the Fermi Gamma-ray Space Telescope have shown that the number of gamma-ray bursts (GRBs) with high-energy photon emission above 100 MeV cannot exceed roughly 9% of the total number of all such events, at least at the present detection limits. In a recent paper, we found that GRBs with photons detected in the Large Area Telescope have a surprisingly broad distribution with respect to the observed event photon number. Extrapolation of our empirical fit to numbers of photons below our previous detection limit suggests that the overall rate of such low flux events could be estimated by standard image co-adding techniques. In this case, we have taken advantage of the excellent angular resolution of the Swift mission to provide accurate reference points for 79 GRB events which have eluded any previous correlations with high-energy photons. We find a small but significant signal in the co-added field. Guided by the extrapolated power-law fit previously obtained for the number distribution of GRBs with higher fluxes, the data suggest that only a small fraction of GRBs are sources of high-energy photons.

  11. The Gamma-Ray Emitting Radio-Loud Narrow-Line Seyfert 1 Galaxy PKS 2004-447 II. The Radio View

    NASA Technical Reports Server (NTRS)

    Schulz, R.; Kreikenbohm, A.; Kadler, M.; Ojha, R.; Ros, E.; Stevens, J.; Edwards, P. G.; Carpenter, B.; Elsaesser, D.; Gehrels, N.; hide

    2016-01-01

    Context. gamma-ray-detected radio-loud narrow-line Seyfert 1 (gamma-NLS1) galaxies constitute a small but interesting sample of the gamma-ray-loud AGN. The radio-loudest gamma-NLS1 known, PKS2004447, is located in the southern hemisphere and is monitored in the radio regime by the multiwavelength monitoring programme TANAMI. Aims. We aim for the first detailed study of the radio morphology and long-term radio spectral evolution of PKS2004447, which are essential for understanding the diversity of the radio properties of gamma-NLS1s. Methods. The TANAMI VLBI monitoring program uses the Australian Long Baseline Array (LBA) and telescopes in Antarctica, Chile, New Zealand, and South Africa to monitor the jets of radio-loud active galaxies in the southern hemisphere. Lower resolution radio flux density measurements at multiple radio frequencies over four years of observations were obtained with the Australia Telescope Compact Array (ATCA). Results. The TANAMI VLBI image at 8.4GHz shows an extended one-sided jet with a dominant compact VLBI core. Its brightness temperature is consistent with equipartition, but it is an order of magnitude below other gamma-NLS1s with the sample value varying over two orders of magnitude. We find a compact morphology with a projected large-scale size 11 kpc and a persistent steep radio spectrum with moderate flux-density variability. Conclusions. PKS2004447 appears to be a unique member of the gamma-NLS1 sample. It exhibits blazar-like features, such as a flat featureless X-ray spectrum and a core-dominated, one-sided parsec-scale jet with indications for relativistic beaming. However, the data also reveal properties atypical for blazars, such as a radio spectrum and large-scale size consistent with compact-steep-spectrum (CSS) objects, which are usually associated with young radio sources. These characteristics are unique among all gamma-NLS1s and extremely rare among gamma-ray-loud AGN.

  12. A Gamma-Ray Burst Model Via Compressional Heating of Binary Neutron Stars

    NASA Astrophysics Data System (ADS)

    Salmonson, J. D.; Wilson, J. R.; Mathews, G. J.

    1998-12-01

    We present a model for gamma-ray bursts based on the compression of neutron stars in close binary systems. General relativistic (GR) simulations of close neutron star binaries have found compression of the neutron stars estimated to produce 1053 ergs of thermal neutrinos on a timescale of seconds. The hot neutron stars will emit neutrino pairs which will partially recombine to form 1051 to 1052 ergs of electron-positron (e^-e^+) pair plasma. GR hydrodynamic computational modeling of the e^-e^+ plasma flow and recombination yield a gamma-ray burst in good agreement with general characteristics (duration ~10 seconds, spectrum peak energy ~100 keV, total energy ~1051 ergs) of many observed gamma-ray bursts.

  13. Modulated high-energy gamma-ray emission from the microquasar Cygnus X-3.

    PubMed

    Abdo, A A; Ackermann, M; Ajello, M; Axelsson, M; Baldini, L; Ballet, J; Barbiellini, G; Bastieri, D; Baughman, B M; Bechtol, K; Bellazzini, R; Berenji, B; Blandford, R D; Bloom, E D; Bonamente, E; Borgland, A W; Brez, A; Brigida, M; Bruel, P; Burnett, T H; Buson, S; Caliandro, G A; Cameron, R A; Caraveo, P A; Casandjian, J M; Cecchi, C; Celik, O; Chaty, S; Cheung, C C; Chiang, J; Ciprini, S; Claus, R; Cohen-Tanugi, J; Cominsky, L R; Conrad, J; Corbel, S; Corbet, R; Dermer, C D; de Palma, F; Digel, S W; do Couto e Silva, E; Drell, P S; Dubois, R; Dubus, G; Dumora, D; Farnier, C; Favuzzi, C; Fegan, S J; Focke, W B; Fortin, P; Frailis, M; Fusco, P; Gargano, F; Gehrels, N; Germani, S; Giavitto, G; Giebels, B; Giglietto, N; Giordano, F; Glanzman, T; Godfrey, G; Grenier, I A; Grondin, M-H; Grove, J E; Guillemot, L; Guiriec, S; Hanabata, Y; Harding, A K; Hayashida, M; Hays, E; Hill, A B; Hjalmarsdotter, L; Horan, D; Hughes, R E; Jackson, M S; Jóhannesson, G; Johnson, A S; Johnson, T J; Johnson, W N; Kamae, T; Katagiri, H; Kawai, N; Kerr, M; Knödlseder, J; Kocian, M L; Koerding, E; Kuss, M; Lande, J; Latronico, L; Lemoine-Goumard, M; Longo, F; Loparco, F; Lott, B; Lovellette, M N; Lubrano, P; Madejski, G M; Makeev, A; Marchand, L; Marelli, M; Max-Moerbeck, W; Mazziotta, M N; McColl, N; McEnery, J E; Meurer, C; Michelson, P F; Migliari, S; Mitthumsiri, W; Mizuno, T; Monte, C; Monzani, M E; Morselli, A; Moskalenko, I V; Murgia, S; Nolan, P L; Norris, J P; Nuss, E; Ohsugi, T; Omodei, N; Ong, R A; Ormes, J F; Paneque, D; Parent, D; Pelassa, V; Pepe, M; Pesce-Rollins, M; Piron, F; Pooley, G; Porter, T A; Pottschmidt, K; Rainò, S; Rando, R; Ray, P S; Razzano, M; Rea, N; Readhead, A; Reimer, A; Reimer, O; Richards, J L; Rochester, L S; Rodriguez, J; Rodriguez, A Y; Romani, R W; Ryde, F; Sadrozinski, H F-W; Sander, A; Saz Parkinson, P M; Sgrò, C; Siskind, E J; Smith, D A; Smith, P D; Spinelli, P; Starck, J-L; Stevenson, M; Strickman, M S; Suson, D J; Takahashi, H; Tanaka, T; Thayer, J B; Thompson, D J; Tibaldo, L; Tomsick, J A; Torres, D F; Tosti, G; Tramacere, A; Uchiyama, Y; Usher, T L; Vasileiou, V; Vilchez, N; Vitale, V; Waite, A P; Wang, P; Wilms, J; Winer, B L; Wood, K S; Ylinen, T; Ziegler, M

    2009-12-11

    Microquasars are accreting black holes or neutron stars in binary systems with associated relativistic jets. Despite their frequent outburst activity, they have never been unambiguously detected emitting high-energy gamma rays. The Fermi Large Area Telescope (LAT) has detected a variable high-energy source coinciding with the position of the x-ray binary and microquasar Cygnus X-3. Its identification with Cygnus X-3 is secured by the detection of its orbital period in gamma rays, as well as the correlation of the LAT flux with radio emission from the relativistic jets of Cygnus X-3. The gamma-ray emission probably originates from within the binary system, opening new areas in which to study the formation of relativistic jets.

  14. Direct fluorination of phenolsulfonphthalein: a method for synthesis of positron-emitting indicators for in vivo pH measurement

    PubMed Central

    Kachur, Alexander V.; Popov, Anatoliy V.; Karp, Joel S.; Delikatny, E. James

    2014-01-01

    We report a reaction of direct electrophilic fluorination of phenolsulfonphthalein at mild conditions. This reaction affords the synthesis of novel positron-emitting 18F-labeled pH indicators. These compounds are useful for non-invasive in vivo pH measurement in biological objects. PMID:22790882

  15. ICF Gamma-Ray measurements on the NIF

    NASA Astrophysics Data System (ADS)

    Herrmann, Hans; Kim, Y.; Hoffman, N. M.; Batha, S. H.; Stoeffl, W.; Church, J. A.; Sayre, D. B.; Liebman, J. A.; Cerjan, C. J.; Carpenter, A. C.; Grafil, E. M.; Khater, H. Y.; Horsfield, C. J.; Rubery, M.

    2013-10-01

    The primary objective of the NIF Gamma Reaction History (GRH) diagnostic is to provide bang time and burn width information in order to constrain implosion simulation parameters such as shell velocity and confinement time. This is accomplished by measuring DT fusion gamma-rays with energy-thresholded Gas Cherenkov detectors that convert MeV gamma-rays into UV/visible photons for high-bandwidth optical detection. Burn-weighted CH ablator areal density is also inferred based on measurement of the 12C(n,n') gammas emitted at 4.44 MeV from DT neutrons inelastically scattering off carbon nuclei as they pass through the plastic ablator. This requires that the four independent GRH gas cells be set to differing Cherenkov thresholds (e.g., 2.9, 4.5, 8 & 10 MeV) in order to be able to unfold the primary spectral components predicted to be in the gamma ray energy spectrum (i.e., DT γ 27Al & 28Si (n,n') γ from the thermo-mechanical package (TMP); and 12C(n,n' γ from the ablator). The GRH response to 12C(n,n') γ is calibrated in-situ by placing a known areal density of carbon in the form of a puck placed ~6 cm from a DT exploding pusher implosion. Comparisons between inferred gamma fluences and simulations based on the nuclear cross sections databases will be presented. Supported by US DOE NNSA.

  16. Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels

    DOEpatents

    Glazer, Alexander N.; Mathies, Richard A.; Hung, Su-Chun; Ju, Jingyue

    1998-01-01

    Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.

  17. No-carrier-added labeling of the neuroprotective Ebselen with selenium-73 and selenium-75.

    PubMed

    Helfer, Andreas; Ermert, Johannes; Humpert, Sven; Coenen, Heinz H

    2015-03-01

    Selenium-73 is a positron emitting non-standard radionuclide, which is suitable for positron emission tomography. A copper-catalyzed reaction allowed no-carrier-added labeling of the anti-inflammatory seleno-organic compound Ebselen with (73) Se and (75) Se under addition of sulfur carrier in a one-step reaction. The new authentically labeled radioselenium molecule is thus available for preclinical evaluation and positron emission tomography studies. Copyright © 2015 John Wiley & Sons, Ltd.

  18. Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels

    DOEpatents

    Glazer, A.N.; Mathies, R.A.; Hung, S.C.; Ju, J.

    1998-12-29

    Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures. 22 figs.

  19. A change in the optical polarization associated with a gamma-ray flare in the blazar 3C 279.

    PubMed

    2010-02-18

    It is widely accepted that strong and variable radiation detected over all accessible energy bands in a number of active galaxies arises from a relativistic, Doppler-boosted jet pointing close to our line of sight. The size of the emitting zone and the location of this region relative to the central supermassive black hole are, however, poorly known, with estimates ranging from light-hours to a light-year or more. Here we report the coincidence of a gamma (gamma)-ray flare with a dramatic change of optical polarization angle. This provides evidence for co-spatiality of optical and gamma-ray emission regions and indicates a highly ordered jet magnetic field. The results also require a non-axisymmetric structure of the emission zone, implying a curved trajectory for the emitting material within the jet, with the dissipation region located at a considerable distance from the black hole, at about 10(5) gravitational radii.

  20. BC404 scintillators as gamma locators studied via Geant4 simulations

    NASA Astrophysics Data System (ADS)

    Cortés, M. L.; Hoischen, R.; Eisenhauer, K.; Gerl, J.; Pietralla, N.

    2014-05-01

    In many applications in industry and academia, an accurate determination of the direction from where gamma rays are emitted is either needed or desirable. Ion-beam therapy treatments, the search for orphan sources, and homeland security applications are examples of fields that can benefit from directional sensitivity to gamma-radiation. Scintillation detectors are a good option for these types of applications as they have relatively low cost, are easy to handle and can be produced in a large range of different sizes. In this work a Geant4 simulation was developed to study the directional sensitivity of different BC404 scintillator geometries and arrangements. The simulation includes all the physical processes relevant for gamma detection in a scintillator. In particular, the creation and propagation of optical photons inside the scintillator was included. A simplified photomultiplier tube model was also simulated. The physical principle exploited is the angular dependence of the shape of the energy spectrum obtained from thin scintillator layers when irradiated from different angles. After an experimental confirmation of the working principle of the device and a check of the simulation, the possibilities and limitations of directional sensitivity to gamma radiation using scintillator layers was tested. For this purpose, point-like sources of typical energies expected in ion-beam therapy were used. Optimal scintillator thicknesses for different energies were determined and the setup efficiencies calculated. The use of arrays of scintillators to reconstruct the direction of incoming gamma rays was also studied. For this case, a spherical source emitting Bremsstrahlung radiation was used together with a setup consisting of scintillator layers. The capability of this setup to identify the center of the extended source was studied together with its angular resolution.

  1. Simulation of prompt gamma-ray emission during proton radiotherapy.

    PubMed

    Verburg, Joost M; Shih, Helen A; Seco, Joao

    2012-09-07

    The measurement of prompt gamma rays emitted from proton-induced nuclear reactions has been proposed as a method to verify in vivo the range of a clinical proton radiotherapy beam. A good understanding of the prompt gamma-ray emission during proton therapy is key to develop a clinically feasible technique, as it can facilitate accurate simulations and uncertainty analysis of gamma detector designs. Also, the gamma production cross-sections may be incorporated as prior knowledge in the reconstruction of the proton range from the measurements. In this work, we performed simulations of proton-induced nuclear reactions with the main elements of human tissue, carbon-12, oxygen-16 and nitrogen-14, using the nuclear reaction models of the GEANT4 and MCNP6 Monte Carlo codes and the dedicated nuclear reaction codes TALYS and EMPIRE. For each code, we made an effort to optimize the input parameters and model selection. The results of the models were compared to available experimental data of discrete gamma line cross-sections. Overall, the dedicated nuclear reaction codes reproduced the experimental data more consistently, while the Monte Carlo codes showed larger discrepancies for a number of gamma lines. The model differences lead to a variation of the total gamma production near the end of the proton range by a factor of about 2. These results indicate a need for additional theoretical and experimental study of proton-induced gamma emission in human tissue.

  2. Method of making colloid labeled with radionuclide

    DOEpatents

    Atcher, Robert W.; Hines, John J.

    1991-01-01

    A ferric hydroxide colloid having an alpha-emitting radionuclide essentially on the outer surfaces and a method of forming same. The method includes oxidizing a ferrous hydroxide to ferric hydroxide in the presence of a preselected radionuclide to form a colloid having the radionuclide on the outer surface thereof, and thereafter washing the colloid, and suspending the washed colloid in a suitable solution. The labelled colloid is useful in cancer therapy and for the treatment of inflamed joints.

  3. Fast variability of tera-electron volt gamma rays from the radio galaxy M87.

    PubMed

    Aharonian, F; Akhperjanian, A G; Bazer-Bachi, A R; Beilicke, M; Benbow, W; Berge, D; Bernlöhr, K; Boisson, C; Bolz, O; Borrel, V; Braun, I; Brown, A M; Bühler, R; Büsching, I; Carrigan, S; Chadwick, P M; Chounet, L-M; Coignet, G; Cornils, R; Costamante, L; Degrange, B; Dickinson, H J; Djannati-Ataï, A; Drury, L O'c; Dubus, G; Egberts, K; Emmanoulopoulos, D; Espigat, P; Feinstein, F; Ferrero, E; Fiasson, A; Fontaine, G; Funk, Seb; Funk, S; Füssling, M; Gallant, Y A; Giebels, B; Glicenstein, J F; Goret, P; Hadjichristidis, C; Hauser, D; Hauser, M; Heinzelmann, G; Henri, G; Hermann, G; Hinton, J A; Hoffmann, A; Hofmann, W; Holleran, M; Hoppe, S; Horns, D; Jacholkowska, A; de Jager, O C; Kendziorra, E; Kerschhaggl, M; Khélifi, B; Komin, Nu; Konopelko, A; Kosack, K; Lamanna, G; Latham, I J; Le Gallou, R; Lemière, A; Lemoine-Goumard, M; Lenain, J-P; Lohse, T; Martin, J M; Martineau-Huynh, O; Marcowith, A; Masterson, C; Maurin, G; McComb, T J L; Moulin, E; de Naurois, M; Nedbal, D; Nolan, S J; Noutsos, A; Orford, K J; Osborne, J L; Ouchrif, M; Panter, M; Pelletier, G; Pita, S; Pühlhofer, G; Punch, M; Ranchon, S; Raubenheimer, B C; Raue, M; Rayner, S M; Reimer, A; Ripken, J; Rob, L; Rolland, L; Rosier-Lees, S; Rowell, G; Sahakian, V; Santangelo, A; Saugé, L; Schlenker, S; Schlickeiser, R; Schröder, R; Schwanke, U; Schwarzburg, S; Schwemmer, S; Shalchi, A; Sol, H; Spangler, D; Spanier, F; Steenkamp, R; Stegmann, C; Superina, G; Tam, P H; Tavernet, J-P; Terrier, R; Tluczykont, M; van Eldik, C; Vasileiadis, G; Venter, C; Vialle, J P; Vincent, P; Völk, H J; Wagner, S J; Ward, M

    2006-12-01

    The detection of fast variations of the tera-electron volt (TeV) (10(12) eV) gamma-ray flux, on time scales of days, from the nearby radio galaxy M87 is reported. These variations are about 10 times as fast as those observed in any other wave band and imply a very compact emission region with a dimension similar to the Schwarzschild radius of the central black hole. We thus can exclude several other sites and processes of the gamma-ray production. The observations confirm that TeV gamma rays are emitted by extragalactic sources other than blazars, where jets are not relativistically beamed toward the observer.

  4. Monitoring the distribution of prompt gamma rays in boron neutron capture therapy using a multiple-scattering Compton camera: A Monte Carlo simulation study

    NASA Astrophysics Data System (ADS)

    Lee, Taewoong; Lee, Hyounggun; Lee, Wonho

    2015-10-01

    This study evaluated the use of Compton imaging technology to monitor prompt gamma rays emitted by 10B in boron neutron capture therapy (BNCT) applied to a computerized human phantom. The Monte Carlo method, including particle-tracking techniques, was used for simulation. The distribution of prompt gamma rays emitted by the phantom during irradiation with neutron beams is closely associated with the distribution of the boron in the phantom. Maximum likelihood expectation maximization (MLEM) method was applied to the information obtained from the detected prompt gamma rays to reconstruct the distribution of the tumor including the boron uptake regions (BURs). The reconstructed Compton images of the prompt gamma rays were combined with the cross-sectional images of the human phantom. Quantitative analysis of the intensity curves showed that all combined images matched the predetermined conditions of the simulation. The tumors including the BURs were distinguishable if they were more than 2 cm apart.

  5. Chandra Contributes to ESA's Integral Detection of Closest Gamma-Ray Burst

    NASA Astrophysics Data System (ADS)

    2004-08-01

    A gamma-ray burst detected by ESA's Integral gamma-ray observatory on 3 December 2003 has been thoroughly studied for months by an armada of space and ground-based observatories. Astronomers have now concluded that this event, called GRB 031203, is the closest cosmic gamma-ray burst on record, and also the faintest. This also suggests that an entire population of sub-energetic gamma-ray bursts has so far gone unnoticed. Cosmic gamma-ray bursts (GRBs) are flashes of gamma rays that can last from less than a second to a few minutes and occur at random positions in the sky. A large fraction of them is thought to result when a black hole is created from a dying star in a distant galaxy. Astronomers believe that a hot disc surrounding the black hole, made of gas and matter falling onto it, somehow emits an energetic beam parallel to the axis of rotation. According to the simplest picture, all GRBs should emit similar amounts of gamma-ray energy. The fraction of it detected at Earth should then depend on the 'width' (opening angle) and orientation of the beam as well as on the distance. The energy received should be larger when the beam is narrow or points towards us and smaller when the beam is broad or points away from us. New data collected with ESA's high energy observatories, Integral and XMM-Newton, now show that this picture is not so clear-cut and that the amount of energy emitted by GRBs can vary significantly. "The idea that all GRBs spit out the same amount of gamma rays, or that they are 'standard candles' as we call them, is simply ruled out by the new data," said Dr Sergey Sazonov, from the Space Research Institute of the Russian Academy of Sciences, Moscow (Russia) and the Max-Planck Institute for Astrophysics, Garching near Munich (Germany). Sazonov and an international team of researchers studied the GRB detected by Integral on 3 December 2003 and given the code-name of GRB 031203. Within a record 18 seconds of the burst, the Integral Burst Alert System

  6. Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores

    DOEpatents

    Glazer, Alexander N.; Mathies, Richard A.; Hung, Su-Chun; Ju, Jingyue

    2000-01-01

    Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.

  7. 10 CFR 35.600 - Use of a sealed source in a remote afterloader unit, teletherapy unit, or gamma stereotactic...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and..., teletherapy unit, or gamma stereotactic radiosurgery unit. A licensee shall use sealed sources in photon...

  8. 10 CFR 35.600 - Use of a sealed source in a remote afterloader unit, teletherapy unit, or gamma stereotactic...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and..., teletherapy unit, or gamma stereotactic radiosurgery unit. A licensee shall use sealed sources in photon...

  9. 10 CFR 35.600 - Use of a sealed source in a remote afterloader unit, teletherapy unit, or gamma stereotactic...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and..., teletherapy unit, or gamma stereotactic radiosurgery unit. A licensee shall use sealed sources in photon...

  10. 10 CFR 35.600 - Use of a sealed source in a remote afterloader unit, teletherapy unit, or gamma stereotactic...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and..., teletherapy unit, or gamma stereotactic radiosurgery unit. A licensee shall use sealed sources in photon...

  11. 10 CFR 35.600 - Use of a sealed source in a remote afterloader unit, teletherapy unit, or gamma stereotactic...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and..., teletherapy unit, or gamma stereotactic radiosurgery unit. A licensee shall use sealed sources in photon...

  12. Photoaffinity Labeling of Developing Jojoba Seed Microsomal Membranes with a Photoreactive Analog of Acyl-Coenzyme A (Acyl-CoA) (Identification of a Putative Acyl-CoA:Fatty Alcohol Acyltransferase.

    PubMed

    Shockey, J. M.; Rajasekharan, R.; Kemp, J. D.

    1995-01-01

    Jojoba (Simmondsia chinensis, Link) is the only plant known that synthesizes liquid wax. The final step in liquid wax biosynthesis is catalyzed by an integral membrane enzyme, fatty acyl-coenzyme A (CoA):fatty alcohol acyltransferase, which transfers an acyl chain from acyl-CoA to a fatty alcohol to form the wax ester. To purify the acyltransferase, we have labeled the enzyme with a radioiodinated, photoreactive analog of acyl-CoA, 12-[N-(4-azidosalicyl)amino] dodecanoyl-CoA (ASD-CoA). This molecule acts as an inhibitor of acyltransferase activity in the dark and as an irreversible inhibitor upon exposure to ultraviolet light. Oleoyl-CoA protects enzymatic activity in a concentration-dependent manner. Photolysis of microsomal membranes with labeled ASD-CoA resulted in strong labeling of two polypeptides of 57 and 52 kD. Increasing concentrations of oleoyl-CoA reduced the labeling of the 57-kD polypeptide dramatically, whereas the labeling of the 52-kD polypeptide was much less responsive to oleoyl-CoA. Also, unlike the other polypeptide, the labeling of the 57-kD polypeptide was enhanced considerably when photolyzed in the presence of dodecanol. These results suggest that a 57-kD polypeptide from jojoba microsomes may be the acyl-CoA:fatty alcohol acyltransferase.

  13. Sky and Elemental Planetary Mapping Via Gamma Ray Emissions

    NASA Technical Reports Server (NTRS)

    Roland, John M.

    2011-01-01

    Low-energy gamma ray emissions ((is) approximately 30keV to (is) approximately 30MeV) are significant to astrophysics because many interesting objects emit their primary energy in this regime. As such, there has been increasing demand for a complete map of the gamma ray sky, but many experiments to do so have encountered obstacles. Using an innovative method of applying the Radon Transform to data from BATSE (the Burst And Transient Source Experiment) on NASA's CGRO (Compton Gamma-Ray Observatory) mission, we have circumvented many of these issues and successfully localized many known sources to 0.5 - 1 deg accuracy. Our method, which is based on a simple 2-dimensional planar back-projection approximation of the inverse Radon transform (familiar from medical CAT-scan technology), can thus be used to image the entire sky and locate new gamma ray sources, specifically in energy bands between 200keV and 2MeV which have not been well surveyed to date. Samples of these results will be presented. This same technique can also be applied to elemental planetary surface mapping via gamma ray spectroscopy. Due to our method's simplicity and power, it could potentially improve a current map's resolution by a significant factor.

  14. Photoaffinity Labeling of Developing Jojoba Seed Microsomal Membranes with a Photoreactive Analog of Acyl-Coenzyme A (Acyl-CoA) (Identification of a Putative Acyl-CoA:Fatty Alcohol Acyltransferase.

    PubMed Central

    Shockey, J. M.; Rajasekharan, R.; Kemp, J. D.

    1995-01-01

    Jojoba (Simmondsia chinensis, Link) is the only plant known that synthesizes liquid wax. The final step in liquid wax biosynthesis is catalyzed by an integral membrane enzyme, fatty acyl-coenzyme A (CoA):fatty alcohol acyltransferase, which transfers an acyl chain from acyl-CoA to a fatty alcohol to form the wax ester. To purify the acyltransferase, we have labeled the enzyme with a radioiodinated, photoreactive analog of acyl-CoA, 12-[N-(4-azidosalicyl)amino] dodecanoyl-CoA (ASD-CoA). This molecule acts as an inhibitor of acyltransferase activity in the dark and as an irreversible inhibitor upon exposure to ultraviolet light. Oleoyl-CoA protects enzymatic activity in a concentration-dependent manner. Photolysis of microsomal membranes with labeled ASD-CoA resulted in strong labeling of two polypeptides of 57 and 52 kD. Increasing concentrations of oleoyl-CoA reduced the labeling of the 57-kD polypeptide dramatically, whereas the labeling of the 52-kD polypeptide was much less responsive to oleoyl-CoA. Also, unlike the other polypeptide, the labeling of the 57-kD polypeptide was enhanced considerably when photolyzed in the presence of dodecanol. These results suggest that a 57-kD polypeptide from jojoba microsomes may be the acyl-CoA:fatty alcohol acyltransferase. PMID:12228351

  15. The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule.

    PubMed

    Garousi, Javad; Andersson, Ken G; Dam, Johan H; Olsen, Birgitte B; Mitran, Bogdan; Orlova, Anna; Buijs, Jos; Ståhl, Stefan; Löfblom, John; Thisgaard, Helge; Tolmachev, Vladimir

    2017-07-20

    Several anti-cancer therapies target the epidermal growth factor receptor (EGFR). Radionuclide imaging of EGFR expression in tumours may aid in selection of optimal cancer therapy. The 111 In-labelled DOTA-conjugated Z EGFR:2377 Affibody molecule was successfully used for imaging of EGFR-expressing xenografts in mice. An optimal combination of radionuclide, chelator and targeting protein may further improve the contrast of radionuclide imaging. The aim of this study was to evaluate the targeting properties of radiocobalt-labelled DOTA-Z EGFR:2377 . DOTA-Z EGFR:2377 was labelled with 57 Co (T 1/2  = 271.8 d), 55 Co (T 1/2  = 17.5 h), and, for comparison, with the positron-emitting radionuclide 68 Ga (T 1/2  = 67.6 min) with preserved specificity of binding to EGFR-expressing A431 cells. The long-lived cobalt radioisotope 57 Co was used in animal studies. Both 57 Co-DOTA-Z EGFR:2377 and 68 Ga-DOTA-Z EGFR:2377 demonstrated EGFR-specific accumulation in A431 xenografts and EGFR-expressing tissues in mice. Tumour-to-organ ratios for the radiocobalt-labelled DOTA-Z EGFR:2377 were significantly higher than for the gallium-labelled counterpart already at 3 h after injection. Importantly, 57 Co-DOTA-Z EGFR:2377 demonstrated a tumour-to-liver ratio of 3, which is 7-fold higher than the tumour-to-liver ratio for 68 Ga-DOTA-Z EGFR:2377 . The results of this study suggest that the positron-emitting cobalt isotope 55 Co would be an optimal label for DOTA-Z EGFR:2377 and further development should concentrate on this radionuclide as a label.

  16. Single Upconversion Nanoparticle-Bacterium Cotrapping for Single-Bacterium Labeling and Analysis.

    PubMed

    Xin, Hongbao; Li, Yuchao; Xu, Dekang; Zhang, Yueli; Chen, Chia-Hung; Li, Baojun

    2017-04-01

    Detecting and analyzing pathogenic bacteria in an effective and reliable manner is crucial for the diagnosis of acute bacterial infection and initial antibiotic therapy. However, the precise labeling and analysis of bacteria at the single-bacterium level are a technical challenge but very important to reveal important details about the heterogeneity of cells and responds to environment. This study demonstrates an optical strategy for single-bacterium labeling and analysis by the cotrapping of single upconversion nanoparticles (UCNPs) and bacteria together. A single UCNP with an average size of ≈120 nm is first optically trapped. Both ends of a single bacterium are then trapped and labeled with single UCNPs emitting green light. The labeled bacterium can be flexibly moved to designated locations for further analysis. Signals from bacteria of different sizes are detected in real time for single-bacterium analysis. This cotrapping method provides a new approach for single-pathogenic-bacterium labeling, detection, and real-time analysis at the single-particle and single-bacterium level. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Altered polymorphonuclear leukocyte Fc gamma R expression contributes to decreased candicidal activity during intraabdominal sepsis

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Simms, H.H.; D'Amico, R.; Monfils, P.

    We investigated the effects of untreated intraabdominal sepsis on polymorphonuclear leukocyte (PMN) candicidal activity. Two groups of swine were studied. Group I (n=6) underwent sham laparotomy, group II (n=7) underwent cecal ligation and incision. Untreated intraabdominal sepsis resulted in a progressive decrease in PMN candicidal activity. Concomitant rosetting and phagocytosis assays demonstrated a decrease in both the attachment and phagocytosis of Candida albicans opsonized with both normal and septic swine serum by PMNs in group II. Iodine 125-labeled swine immunoglobulin G (IgG) and fluorescein isothioalanate (FITC)-labeled swine IgG were used to investigate Fc gamma receptor ligand interactions. Scatchard analyses demonstratedmore » a progressive decline in both the binding affinity constant and number of IgG molecules bound per PMN. Stimulation of the oxidative burst markedly reduced 125I-labeled IgG binding in both group I and group II, with a greater decrement being seen in animals with intraabdominal sepsis. Further, in group II, PMN recycling of the Fc gamma receptor to the cell surface after generation of the oxidative burst was reduced by postoperative day 4. Binding of monoclonal antibodies to Fc gamma receptor II, but not Fc gamma receptor I/III markedly reduced intracellular candicidal activity. Immunofluorescence studies revealed a homogeneous pattern of FITC-IgG uptake by nearly all group I PMNs, whereas by postoperative day 8 a substantial number of PMNs from group II failed to internalize the FITC-IgG. These studies suggest that untreated intraabdominal sepsis reduces PMN candicidal activity and that this is due, in part, to altered PMN Fc gamma receptor ligand interactions.« less

  18. The hump in the Cerenkov lateral distribution of gamma ray showers

    NASA Technical Reports Server (NTRS)

    Sinha, S.; Sao, M. V. S.

    1985-01-01

    The lateral distribution of atmospheric Cerenkov photons emitted by gamma ray showers of energy 100 GeV is calculated. The lateral distribution shows a characteristic hump at a distance of approx. 135 meter from the core. The hump is shown to be due to electrons of threshold energy 1 GeV, above which the mean scattering angle becomes smaller than the Cerenkov angle.

  19. Nicotinic acetylcholine receptor probed with a photoactivatable agonist: improved labeling specificity by addition of CeIV/glutathione. Extension to laser flash photolabeling.

    PubMed

    Grutter, T; Goeldner, M; Kotzyba-Hibert, F

    1999-06-08

    The molecular structure of Torpedo marmorata acetylcholine binding sites has been investigated previously by photoaffinity labeling. However, besides the nicotine molecule [Middleton et al. (1991) Biochemistry 30, 6987-6997], all other photosensitive probes used for this purpose interacted only with closed receptor states. In the perspective of mapping the functional activated state, we synthesized and developed a new photoactivatable agonist of nAChR capable of alkylation of the acetylcholine (ACh) binding sites, as reported previously [Kotzyba-Hibert et al. (1997) Bioconjugate Chem. 8, 472-480]. Here, we describe the setup of experimental conditions that were made in order to optimize the photolabeling reaction and in particular its specificity. We found that subsequent addition of the oxidant ceric ion (CeIV) and reduced glutathione before the photolabeling step lowered considerably nonspecific labeling (over 90% protection with d-tubocurarine) without affecting the binding properties of the ACh binding sites. As a consequence, irradiation at 360 nm for 20 min in these new conditions gave satisfactory coupling yields (7.5%). A general mechanism was proposed to explain the successive reactions occurring and their drastic effect on the specificity of the labeling reaction. Last, these incubation conditions can be extended to nanosecond pulsed laser photolysis leading to the same specific photoincorporation as for usual irradiations (8.5% coupling yield of ACh binding sites, 77% protection with carbamylcholine). Laser flash photocoupling of a diazocyclohexadienoyl probe on nAChR was achieved for the first time. Taken together, these data indicate that future investigation of the molecular dynamics of allosteric transitions occurring at the activated ACh binding sites should be possible.

  20. Gamma-ray bursts at high and very high energies

    NASA Astrophysics Data System (ADS)

    Piron, Frédéric

    2016-06-01

    Gamma-Ray Bursts (GRBs) are extra-galactic and extremely energetic transient emissions of gamma rays, which are thought to be associated with the death of massive stars or the merger of compact objects in binary systems. Their huge luminosities involve the presence of a newborn stellar-mass black hole emitting a relativistic collimated outflow, which accelerates particles and produces non-thermal emissions from the radio domain to the highest energies. In this article, I review recent progresses in the understanding of GRB jet physics above 100 MeV, based on Fermi observations of bright GRBs. I discuss the physical implications of these observations and their impact on GRB modeling, and I present some prospects for GRB observation at very high energies in the near future.

  1. 10 CFR 35.615 - Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.615 Section 35.615 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and...

  2. 10 CFR 35.615 - Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.615 Section 35.615 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and...

  3. 10 CFR 35.615 - Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.615 Section 35.615 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and...

  4. 10 CFR 35.615 - Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Safety precautions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.615 Section 35.615 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units, Teletherapy Units, and...

  5. Time evolution of gamma rays from supernova remnants

    NASA Astrophysics Data System (ADS)

    Gaggero, Daniele; Zandanel, Fabio; Cristofari, Pierre; Gabici, Stefano

    2018-04-01

    We present a systematic phenomenological study focused on the time evolution of the non-thermal radiation - from radio waves to gamma rays - emitted by typical supernova remnants via hadronic and leptonic mechanisms, for two classes of progenitors: thermonuclear and core-collapse. To this aim, we develop a numerical tool designed to model the evolution of the cosmic ray spectrum inside a supernova remnant, and compute the associated multi-wavelength emission. We demonstrate the potential of this tool in the context of future population studies based on large collection of high-energy gamma-ray data. We discuss and explore the relevant parameter space involved in the problem, and focus in particular on their impact on the maximum energy of accelerated particles, in order to study the effectiveness and duration of the PeVatron phase. We outline the crucial role of the ambient medium through which the shock propagates during the remnant evolution. In particular, we point out the role of dense clumps in creating a significant hardening in the hadronic gamma-ray spectrum.

  6. Sizing up the population of gamma-ray binaries

    NASA Astrophysics Data System (ADS)

    Dubus, Guillaume; Guillard, Nicolas; Petrucci, Pierre-Olivier; Martin, Pierrick

    2017-12-01

    Context. Gamma-ray binaries are thought to be composed of a young pulsar in orbit around a massive O or Be star with their gamma-ray emission powered by pulsar spin-down. The number of such systems in our Galaxy is not known. Aims: We aim to estimate the total number of gamma-ray binaries in our Galaxy and to evaluate the prospects for new detections in the GeV and TeV energy range, taking into account that their gamma-ray emission is modulated on the orbital period. Methods: We modelled the population of gamma-ray binaries and evaluated the fraction of detected systems in surveys with the Fermi-LAT (GeV), H.E.S.S., HAWC and CTA (TeV) using observation-based and synthetic template light curves. Results: The detected fraction depends more on the orbit-average flux than on the light-curve shape. Our best estimate for the number of gamma-ray binaries is 101-52+89 systems. A handful of discoveries are expected by pursuing the Fermi-LAT survey. Discoveries in TeV surveys are less likely. However, this depends on the relative amounts of power emitted in GeV and TeV domains. There could be as many as ≈ 200 HESS J0632+057-like systems with a high ratio of TeV to GeV emission compared to other gamma-ray binaries. Statistics allow for as many as three discoveries in five years of HAWC observations and five discoveries in the first two years of the CTA Galactic Plane survey. Conclusions: We favour continued Fermi-LAT observations over ground-based TeV surveys to find new gamma-ray binaries. Gamma-ray observations are most sensitive to short orbital period systems with a high spin-down pulsar power. Radio pulsar surveys (SKA) are likely to be more efficient in detecting long orbital period systems, providing a complementary probe into the gamma-ray binary population.

  7. Simulating Gamma-Ray Emission in Star-forming Galaxies

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pfrommer, Christoph; Pakmor, Rüdiger; Simpson, Christine M.

    Star-forming galaxies emit GeV and TeV gamma-rays that are thought to originate from hadronic interactions of cosmic-ray (CR) nuclei with the interstellar medium. To understand the emission, we have used the moving-mesh code Arepo to perform magnetohydrodynamical galaxy formation simulations with self-consistent CR physics. Our galaxy models exhibit a first burst of star formation that injects CRs at supernovae. Once CRs have sufficiently accumulated in our Milky Way–like galaxy, their buoyancy force overcomes the magnetic tension of the toroidal disk field. As field lines open up, they enable anisotropically diffusing CRs to escape into the halo and to accelerate amore » bubble-like, CR-dominated outflow. However, these bubbles are invisible in our simulated gamma-ray maps of hadronic pion-decay and secondary inverse-Compton emission because of low gas density in the outflows. By adopting a phenomenological relation between star formation rate (SFR) and far-infrared emission and assuming that gamma-rays mainly originate from decaying pions, our simulated galaxies can reproduce the observed tight relation between far-infrared and gamma-ray emission, independent of whether we account for anisotropic CR diffusion. This demonstrates that uncertainties in modeling active CR transport processes only play a minor role in predicting gamma-ray emission from galaxies. We find that in starbursts, most of the CR energy is “calorimetrically” lost to hadronic interactions. In contrast, the gamma-ray emission deviates from this calorimetric property at low SFRs due to adiabatic losses, which cannot be identified in traditional one-zone models.« less

  8. Simulating Gamma-Ray Emission in Star-forming Galaxies

    NASA Astrophysics Data System (ADS)

    Pfrommer, Christoph; Pakmor, Rüdiger; Simpson, Christine M.; Springel, Volker

    2017-10-01

    Star-forming galaxies emit GeV and TeV gamma-rays that are thought to originate from hadronic interactions of cosmic-ray (CR) nuclei with the interstellar medium. To understand the emission, we have used the moving-mesh code Arepo to perform magnetohydrodynamical galaxy formation simulations with self-consistent CR physics. Our galaxy models exhibit a first burst of star formation that injects CRs at supernovae. Once CRs have sufficiently accumulated in our Milky Way-like galaxy, their buoyancy force overcomes the magnetic tension of the toroidal disk field. As field lines open up, they enable anisotropically diffusing CRs to escape into the halo and to accelerate a bubble-like, CR-dominated outflow. However, these bubbles are invisible in our simulated gamma-ray maps of hadronic pion-decay and secondary inverse-Compton emission because of low gas density in the outflows. By adopting a phenomenological relation between star formation rate (SFR) and far-infrared emission and assuming that gamma-rays mainly originate from decaying pions, our simulated galaxies can reproduce the observed tight relation between far-infrared and gamma-ray emission, independent of whether we account for anisotropic CR diffusion. This demonstrates that uncertainties in modeling active CR transport processes only play a minor role in predicting gamma-ray emission from galaxies. We find that in starbursts, most of the CR energy is “calorimetrically” lost to hadronic interactions. In contrast, the gamma-ray emission deviates from this calorimetric property at low SFRs due to adiabatic losses, which cannot be identified in traditional one-zone models.

  9. Rapid in vitro labeling procedures for two-dimensional gel fingerprinting

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lee, Y.F.; Fowlks, E.R.

    1982-01-15

    Improvements of existing in vitro procedures for labeling RNA radioactively, and modifications of the two-dimensional polyacrylamide gel electrophoresis system for making RNA fingerprints are described. These improvements are (a) inactivation of phosphatase with nitric acid at pH 2.0 eliminated the phenol-cholorform extraction step during 5'-end labeling with polynucleotide kinase and (..gamma..-/sup 32/P)ATP; (b) ZnSO/sub 4/ inactivation of R Nase T/sub 1/ results in a highly efficient procedure for 3'-end labeling with T4 ligase and (5'-/sup 32/P)pCp; and (c) a rapid 4-min procedure for variable quantity range of /sup 125/I and RNA results in a qualitative and quantitative sample for high-molecularmore » weight RNA fingerprinting. Thus, these in vitro procedures become rapid and reproducible when combined with two-dimensional gel electrophoresis which eliminates simultaneously labeled impurities. Each labeling procedure is compared, using tobacco mosaic virus, Brome mosaic virus, and polio RNA. A series of Ap-rich oligonucleotides was discovered in the inner genome of Brome mosaic Virus RNA-3.« less

  10. 10 CFR 35.610 - Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.610 Section 35.610 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units...

  11. 10 CFR 35.610 - Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.610 Section 35.610 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units...

  12. 10 CFR 35.610 - Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.610 Section 35.610 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units...

  13. 10 CFR 35.610 - Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.610 Section 35.610 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units...

  14. 10 CFR 35.610 - Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Safety procedures and instructions for remote afterloader units, teletherapy units, and gamma stereotactic radiosurgery units. 35.610 Section 35.610 Energy NUCLEAR REGULATORY COMMISSION MEDICAL USE OF BYPRODUCT MATERIAL Photon Emitting Remote Afterloader Units...

  15. Relative risk analysis of the use of radiation-emitting medical devices: A preliminary application

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jones, E.D.

    This report describes the development of a risk analysis approach for evaluating the use of radiation-emitting medial devices. This effort was performed by Lawrence Livermore National Laboratory for the US Nuclear Regulatory Commission (NRC). The assessment approach has bee applied to understand the risks in using the Gamma Knife, a gamma irradiation therapy device. This effort represents an initial step to evaluate the potential role of risk analysis for developing regulations and quality assurance requirements in the use of nuclear medical devices. The risk approach identifies and assesses the most likely risk contributors and their relative importance for the medicalmore » system. The approach uses expert screening techniques and relative risk profiling to incorporate the type, quality, and quantity of data available and to present results in an easily understood form.« less

  16. Relativistic particles and gamma-ray in quasars and active galactic nuclei

    NASA Technical Reports Server (NTRS)

    Protheroe, R. J.; Kazanas, D.

    1982-01-01

    A model for a class of quasars and active galactic nuclei is described in which a shock around a massive black hole randomizes the infall kinetic energy of spherically accreting matter producing a nonthermal spectrum of high energy protons. These protons may be responsible for the secondary production (via tau + or - decay) of the radio emitting high energy electrons and also of high energy gamma rays (via pi decay and inverse Compton interactions of the electrons). The correlation between radio and gamma ray emission implied by the model is in good agreement with observations of 3C273. Observation of the flux of high energy neutrinos from quasars may provide a test for the model.

  17. Relativistic particles and gamma-rays in quasars and active galactic nuclei

    NASA Technical Reports Server (NTRS)

    Protheroe, R. J.; Kazanas, D.

    1983-01-01

    A model for a class of quasars and active galactic nuclei is described in which a shock around a massive black hole randomizes the infall kinetic energy of spherically accreting matter producing a nonthermal spectrum of high energy protons. These protons may be responsible for the secondary production (via tau + or - decay) of the radio emitting high energy electrons and also of high energy gamma rays (via Pi decay and inverse Compton interactions of the electrons). The correlation between radio and gamma ray emission implied by the model is in good agreement with observations of 3C273. Observation of the flux of high energy neutrinos from quasars may provide a test for the model.

  18. ESA's Integral detects closest cosmic gamma-ray burst

    NASA Astrophysics Data System (ADS)

    2004-08-01

    5 August 2004 A gamma-ray burst detected by ESA's Integral gamma-ray observatory on 3 December 2003 has been thoroughly studied for months by an armada of space and ground-based observatories. Astronomers have now concluded that this event, called GRB 031203, is the closest cosmic gamma-ray burst on record, but also the faintest. This also suggests that an entire population of sub-energetic gamma-ray bursts has so far gone unnoticed... Gamma ray burst model hi-res Size hi-res: 22 KB Credits: CXC/M. Weiss Artist impression of a low-energy gamma-ray burst This illustration describes a model for a gamma-ray burst, like the one detected by Integral on 3 December 2003 (GRB 031203). A jet of high-energy particles from a rapidly rotating black hole interacts with surrounding matter. Observations with Integral on 3 December 2003 and data on its afterglow, collected afterwards with XMM-Newton, Chandra and the Very Large Array telescope, show that GRB 031203 radiated only a fraction of the energy of normal gamma-ray bursts. Like supernovae, gamma-ray bursts are thought to be produced by the collapse of the core of a massive star. However, while the process leading to supernovae is relatively well understood, astronomers still do not know what happens when a core collapses to form a black hole. The discovery of 'under-energetic' gamma-ray bursts, like GRB 031203, should provide valuable clues as to links between supernovae, black holes and gamma-ray bursts. Lo-res JPG (22 Kb) Hi-res TIFF (5800 Kb) Cosmic gamma-ray bursts (GRBs) are flashes of gamma rays that can last from less than a second to a few minutes and occur at random positions in the sky. A large fraction of them is thought to result when a black hole is created from a dying star in a distant galaxy. Astronomers believe that a hot disc surrounding the black hole, made of gas and matter falling onto it, somehow emits an energetic beam parallel to the axis of rotation. According to the simplest picture, all GRBs

  19. Simultaneous observation of the gamma-ray binary LS I+61 303 with GLAST and Suzaku

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tanaka, Takuya; Fukazawa, Yasushi; Mizuno, Tsunefumi

    2007-07-12

    The gamma-ray binary LS I+61 303 is a bright gamma-ray source, and thus an attracting object for GLAST. We proposed to observe this object with the X-ray satellite Suzaku (AO-2), simultaneously with GLAST, radio wave, and optical spectro-polarimetry, in order to probe the geometrical state of the binary system emitting the gamma-ray radiation, as a function of the binary orbital phase for the first time. This is essential to understand the mechanism of jet production and gamma-ray emission. The idea is not only to measure the multi-band overall continuum shape, but also to make use of continuous monitoring capability ofmore » GLAST, wide X-ray band of Suzaku, and good accessibility of the Kanata optical/NIR telescope (Hiroshima University) with the sensitive optical spectro-polarimetry. Further collaboration with TeV gamma-ray telescopes is also hoped to constrain the jet constitution.« less

  20. Limits to the radiative decays of neutrinos and axions from gamma-ray observations of SN 1987A

    NASA Technical Reports Server (NTRS)

    Kolb, Edward W.; Turner, Michael S.

    1989-01-01

    Gamma-ray observations obtained by the SMM gamma-ray spectrometer in the energy range 4.1-6.4 MeV are used to provide limits on the possible radiative decay of neutrinos and axions emitted by SN 1987A. For branching ratio values for the radiative decay modes of less than about 0.0001, the present limits are more stringent than those based upon the photon flux from decaying relic neutrinos. The data are also used to set an axion mass limit.

  1. Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers

    PubMed Central

    Yamamoto, Kazuki; Chikaoka, Yoko; Hayashi, Gosuke; Sakamoto, Ryosuke; Yamamoto, Ryuji; Sugiyama, Akira; Kodama, Tatsuhiko; Okamoto, Akimitsu; Kawamura, Takeshi

    2015-01-01

    Mass spectrometric proteomics is an effective approach for identifying and quantifying histone post-translational modifications (PTMs) and their binding proteins, especially in the cases of methylation and acetylation. However, another vital PTM, phosphorylation, tends to be poorly quantified because it is easily lost and inefficiently ionized. In addition, PTM binding proteins for phosphorylation are sometimes resistant to identification because of their variable binding affinities. Here, we present our efforts to improve the sensitivity of detection of histone H4 tail peptide phosphorylated at serine 1 (H4S1ph) and our successful identification of an H4S1ph binder candidate by means of a chemical proteomics approach. Our nanoLC-MS/MS system permitted semi-quantitative label-free analysis of histone H4 PTM dynamics of cell cycle-synchronized HeLa S3 cells, including phosphorylation, methylation, and acetylation. We show that H4S1ph abundance on nascent histone H4 unmethylated at lysine 20 (H4K20me0) peaks from late S-phase to M-phase. We also attempted to characterize effects of phosphorylation at H4S1 on protein–protein interactions. Specially synthesized photoaffinity bait peptides specifically captured 14-3-3 proteins as novel H4S1ph binding partners, whose interaction was otherwise undetectable by conventional peptide pull-down experiments. This is the first report that analyzes dynamics of PTM pattern on the whole histone H4 tail during cell cycle and enables the identification of PTM binders with low affinities using high-resolution mass spectrometry and photo-affinity bait peptides. PMID:26819910

  2. X-Ray Emission from an Asymmetric Blast Wave and a Massive White Dwarf in the Gamma Ray Emitting Nova V407 CYG

    NASA Technical Reports Server (NTRS)

    Nelson, Thomas; Donato, Davide; Mukai, Koji; Sokoloski, Jennifer; Chomiuk, Laura

    2012-01-01

    Classical nova events in symbiotic stars, although rare, offer a unique opportunity to probe the interaction between ejecta and a dense environment in stellar explosions. In this work, we use X-ray data obtained with Swift and Suzaku during the recent classical nova outburst in V407 Cyg to explore such an interaction. We find evidence of both equilibrium and non-equilibrium ionization plasmas at the time of peak X-ray brightness, indicating a strong asymmetry in the density of the emitting region. Comparing a simple model to the data, we find that the X-ray evolution is broadly consistent with nova ejecta driving a forward shock into the dense wind of the Mira companion. We detect a highly absorbed soft X-ray component in the spectrum during the first 50 days of the outburst that is consistent with supersoft emission from the nuclear burning white dwarf. The high temperature and short turn off time of this emission component, in addition to the observed breaks in the optical and UV lightcurves, indicate that the white dwarf in the binary is extremely massive. Finally, we explore the connections between the X-ray and GeV-ray evolution, and propose that the gamma ray turn-off is due to the stalling of the forward shock as the ejecta reach the red giant surface.

  3. Gamma ray energy tracking in GRETINA

    NASA Astrophysics Data System (ADS)

    Lee, I. Y.

    2011-10-01

    The next generation of stable and exotic beam accelerators will provide physics opportunities to study nuclei farther away from the line of stability. However, these experiments will be more demanding on instrumentation performance. These come from the lower production rate for more exotic beams, worse beam impurities, and large beam velocity from the fragmentation and inverse reactions. Gamma-ray spectroscopy will be one of the most effective tools to study exotic nuclei. However, to fully exploit the physics reach provided by these new facilities, better gamma-ray detector will be needed. In the last 10 years, a new concept, gamma-ray energy tracking array, was developed. Tracking arrays will increase the detection sensitivity by factors of several hundred compared to current arrays used in nuclear physics research. Particularly, the capability of reconstructing the position of the interaction with millimeters resolution is needed to correct the Doppler broadening of gamma rays emitted from high velocity nuclei. GRETINA is a gamma-ray tracking array which uses 28 Ge crystals, each with 36 segments, to cover ¼ of the 4 π of the 4 π solid angle. The gamma ray tracking technique requires detailed pulse shape information from each of the segments. These pulses are digitized using 14-bit 100 MHz flash ADCs, and digital signal analysis algorithms implemented in the on-board FPGAs provides energy, time and selection of pulse traces. A digital trigger system, provided flexible trigger functions including a fast trigger output, and also allows complicated trigger decisions to be made up to 20 microseconds. Further analyzed, carried out in a computer cluster, determine the energy, time, and three-dimensional positions of all gamma-ray interactions in the array. This information is then utilized, together with the characteristics of Compton scattering and pair-production processes, to track the scattering sequences of the gamma rays. GRETINA construction is completed in

  4. EBL constraints with VERITAS gamma-ray observations

    NASA Astrophysics Data System (ADS)

    Fernandez Alonso, M.; VERITAS Collaboration

    2017-10-01

    The extragalactic background light (EBL) contains all the radiation emitted by nuclear and accretion processes since the epoch of recombination. Direct measurements of the EBL in the near-IR to mid-IR waveband are extremely difficult due mainly to the zodiacal light foreground. Instead, gamma-ray astronomy offers the possibility to indirectly set limits to the EBL by studying the effects of gamma-ray absorption in the spectra of detected sources in the very high energy range (VHE: 100 GeV). These effects can be generally seen in the spectra of VHE blazars as a softening (steepening) of the spectrum and/or abrupt changes in the spectral index or breaks. In this work, we use recent VERITAS data of a group of blazars and apply two methods to derive constraints for the EBL spectral properties. We present preliminary results that will be completed with new observations in the near future to enhance the calculated restrictions to the EBL.

  5. Composition and apparatus for detecting gamma radiation

    DOEpatents

    Hofstetter, Kenneth J.

    1994-01-01

    A gamma radiation detector and a radioluminiscent composition for use therein. The detector includes a radioluminscent composition that emits light in a characteristic wavelength region when exposed to gamma radiation, and means for detecting said radiation. The composition contains a scintillant such as anglesite (PbSO.sub.4) or cerussite (PbCO.sub.3) incorporated into an inert, porous glass matrix via a sol-gel process. Particles of radiation-sensitive scintillant are added to, a sol solution. The mixture is polymerized to form a gel, then dried under conditions that preserve the structural integrity and radiation sensitivity of the scintillant. The final product is a composition containing the uniformly-dispersed scintillant in an inert, optically transparent and highly porous matrix. The composition is chemically inert and substantially impervious to environmental conditions including changes in temperature, air pressure, and so forth. It can be fabricated in cylinders, blocks with holes therethrough for flow of fluid, sheets, surface coatings, pellets or other convenient shapes.

  6. Composition and apparatus for detecting gamma radiation

    DOEpatents

    Hofstetter, K.J.

    1994-08-09

    A gamma radiation detector and a radioluminescent composition for use therein. The detector includes a radioluminescent composition that emits light in a characteristic wavelength region when exposed to gamma radiation, and means for detecting said radiation. The composition contains a scintillant such as anglesite (PbSO[sub 4]) or cerussite (PbCO[sub 3]) incorporated into an inert, porous glass matrix via a sol-gel process. Particles of radiation-sensitive scintillant are added to, a sol solution. The mixture is polymerized to form a gel, then dried under conditions that preserve the structural integrity and radiation sensitivity of the scintillant. The final product is a composition containing the uniformly-dispersed scintillant in an inert, optically transparent and highly porous matrix. The composition is chemically inert and substantially impervious to environmental conditions including changes in temperature, air pressure, and so forth. It can be fabricated in cylinders, blocks with holes therethrough for flow of fluid, sheets, surface coatings, pellets or other convenient shapes. 3 figs.

  7. Reducing Uncertainties in the Production of the Gamma-emitting Nuclei {sup 26}Al, {sup 44}Ti, and {sup 60}Fe in Core-collapse Supernovae by Using Effective Helium Burning Rates

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Austin, Sam M.; West, Christopher; Heger, Alexander, E-mail: austin@nscl.msu.edu, E-mail: christopher.west@metrostate.edu, E-mail: Alexander.Heger@Monash.edu

    2017-04-10

    We have used effective reaction rates (ERRs) for the helium burning reactions to predict the yield of the gamma-emitting nuclei {sup 26}Al, {sup 44}Ti, and {sup 60}Fe in core-collapse supernovae (SNe). The variations in the predicted yields for values of the reaction rates allowed by the ERR are much smaller than obtained previously, and smaller than other uncertainties. A “filter” for SN nucleosynthesis yields based on pre-SN structure was used to estimate the effect of failed SNe on the initial mass function averaged yields; this substantially reduced the yields of all these isotopes, but the predicted yield ratio {sup 60}Fe/{supmore » 26}Al was little affected. The robustness of this ratio is promising for comparison with data, but it is larger than observed in nature; possible causes for this discrepancy are discussed.« less

  8. Development of a high resolution liquid xenon imaging chamber for gamma-ray astronomy

    NASA Technical Reports Server (NTRS)

    Aprile, Elena

    1991-01-01

    The objective was to develop the technology of liquid xenon (LXe) detectors for spectroscopy and imaging of gamma rays from astrophysical sources emitting in the low to medium energy regime. In particular, the technical challenges and the physical processes relevant to the realization of the LXe detector operated as a Time Projection Chamber (TPC) were addressed and studied. Experimental results were obtained on the following topics: (1) long distance drift of free electrons in LXe (purity); (2) scintillation light yield for electrons and alphas in LXe (triggering); and (3) ionization yield for electrons and gamma rays in LXe (energy resolution). The major results from the investigations are summarized.

  9. Prediction of ECS and SSC Models for Flux-Limited Samples of Gamma-Ray Blazars

    NASA Technical Reports Server (NTRS)

    Lister, Matthew L.; Marscher, Alan P.

    1999-01-01

    The external Compton scattering (ECS) and synchrotron self-Compton (SSC) models make distinct predictions for the amount of Doppler boosting of high-energy gamma-rays emitted by Nazar. We examine how these differences affect the predicted properties of active galactic nucleus (AGN) samples selected on the basis of Murray emission. We create simulated flux-limited samples based on the ECS and SSC models, and compare their properties to those of identified EGRET blazars. We find that for small gamma-ray-selected samples, the two models make very similar predictions, and cannot be reliably distinguished. This is primarily due to the fact that not only the Doppler factor, but also the cosmological distance and intrinsic luminosity play a role in determining whether an AGN is included in a flux-limited gamma-ray sample.

  10. Calibration of Ge gamma-ray spectrometers for complex sample geometries and matrices

    NASA Astrophysics Data System (ADS)

    Semkow, T. M.; Bradt, C. J.; Beach, S. E.; Haines, D. K.; Khan, A. J.; Bari, A.; Torres, M. A.; Marrantino, J. C.; Syed, U.-F.; Kitto, M. E.; Hoffman, T. J.; Curtis, P.

    2015-11-01

    A comprehensive study of the efficiency calibration and calibration verification of Ge gamma-ray spectrometers was performed using semi-empirical, computational Monte-Carlo (MC), and transfer methods. The aim of this study was to evaluate the accuracy of the quantification of gamma-emitting radionuclides in complex matrices normally encountered in environmental and food samples. A wide range of gamma energies from 59.5 to 1836.0 keV and geometries from a 10-mL jar to 1.4-L Marinelli beaker were studied on four Ge spectrometers with the relative efficiencies between 102% and 140%. Density and coincidence summing corrections were applied. Innovative techniques were developed for the preparation of artificial complex matrices from materials such as acidified water, polystyrene, ethanol, sugar, and sand, resulting in the densities ranging from 0.3655 to 2.164 g cm-3. They were spiked with gamma activity traceable to international standards and used for calibration verifications. A quantitative method of tuning MC calculations to experiment was developed based on a multidimensional chi-square paraboloid.

  11. Observation of high-energy gamma rays from the quasi-stellar object CTA 102

    NASA Technical Reports Server (NTRS)

    Nolan, P. L.; Bertsch, D. L.; Fichtel, C. E.; Hartman, R. C.; Hunter, S. D.; Kanbach, G.; Kniffen, D. A.; Lin, Y. C.; Mattox, J. R.; Mayer-Hasselwander, H. A.

    1993-01-01

    The quasar CTA 102 (QSO 2230 + 114) was observed four times in 1991-1992 by the EGRET high-energy gamma-ray telescope on the Compton GRO satellite. In the 1992 January 23-February 6 observation, emission was detected at the level (2.4 +/- 0.5) x 10 exp 7 photons/sq cm s (E is greater than 100 MeV). The other observations produced upper limits or detections with lower significance which are consistent with the same flux. The photon spectrum can be represented by a power law with a number index of 2.6 +/- 0.2, the softest so far observed by EGRET. The emitted gamma-ray luminosity, if isotropic, is 5 x 10 exp 47 ergs/s (H(0) = 75 km/s Mpc , q(0) = 0.5), although there are good reasons to believe that the gamma emission is strongly beamed.

  12. Some aspects of the scientific significance of high energy gamma ray astrophysics

    NASA Technical Reports Server (NTRS)

    Fichtel, Carl E.

    1991-01-01

    The attraction of high energy gamma-ray astronomy lies in this radiation relating directly to those processes in astrophysical situations which deviate most from thermo-dynamic equilibrium. Some examples of these phenomena which are known to or expected to emit gamma rays are cosmic rays as they interact in intergalactic space, the high energy particles in the magnetic fields of neutron stars, the death of a black hole, the explosion and residual of a supernova, lumps of Weakly Interacting Massive Particles, energetic solar particles interacting near the sun, and very high energy particles in the extreme conditions associated with active galaxies. Although the intensities are known to be low as seen near the earth, a partially compensating characteristic is that the very penetrating nature of high energy gamma rays increases the probability that they can escape from their origin and reach the solar system.

  13. Radiolabelling of isopeptide N epsilon-(gamma-glutamyl)-L-lysine by conjugation with N-succinimidyl-4-[18F]fluorobenzoate.

    PubMed

    Wüst, F; Hultsch, C; Bergmann, R; Johannsen, B; Henle, T

    2003-07-01

    The isopeptide N(epsilon)-(gamma-glutamyl)-L-lysine 4 was labelled with 18F via N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB). A modified approach for the convenient synthesis of [18F]SFB was used, and [18F]SFB could be obtained in decay-corrected radiochemical yields of 44-53% (n = 20) and radiochemical purity >95% within 40 min after EOB. For labelling N(epsilon)-(gamma-glutamyl)-L-lysine with [18F]SFB the effects of isopeptide concentration, temperature, and pH were studied to determine the optimum reaction conditions. The coupling reaction was shown to be temperature and pH independent while being strongly affected by the isopeptide concentration. Using the optimized labelling conditions, in a typical experiment 1.3GBq of [18F]SFB could be converted into 447MBq (46%, decay-corrected) of [18F]fluorobenzoylated isopeptide within 45 min, including HPLC purification.

  14. A correlation between hard gamma-ray sources and cosmic voids along the line of sight

    DOE PAGES

    Furniss, A.; Sutter, P. M.; Primack, J. R.; ...

    2014-11-25

    We estimate the galaxy density along lines of sight to hard extragalactic gamma-ray sources by correlating source positions on the sky with a void catalog based on the Sloan Digital Sky Survey (SDSS). Extragalactic gamma-ray sources that are detected at very high energy (VHE; E > 100 GeV) or have been highlighted as VHE-emitting candidates in the Fermi Large Area Telescope hard source catalog (together referred to as “VHE-like” sources) are distributed along underdense lines of sight at the 2.4σ level. There is a less suggestive correlation for the Fermi hard source population (1.7σ). A correlation between 10-500 GeV fluxmore » and underdense fraction along the line of sight for VHE-like and Fermi hard sources is found at 2.4σ and 2.6σ, calculated from the Pearson correlation coefficients of r = 0.57 and 0.47, respectively. The preference for underdense sight lines is not displayed by gamma-ray emitting galaxies within the second Fermi catalog, containing sources detected above 100 MeV, or the SDSS DR7 quasar catalog. We investigate whether this marginal correlation might be a result of lower extragalactic background light (EBL) photon density within the underdense regions and find that, even in the most extreme case of a entirely underdense sight line, the EBL photon density is only 2% less than the nominal EBL density. Translating this into gamma-ray attenuation along the line of sight for a highly attenuated source with opacity τ(E, z) ~ 5, we estimate that the attentuation of gamma-rays decreases no more than 10%. This decrease, although non-neglible, is unable to account for the apparent hard source correlation with underdense lines of sight.« less

  15. White light-emitting organic electroluminescent devices

    DOEpatents

    Shiang, Joseph John; Duggal, Anil Raj; Parthasarathy, Gautam

    2006-06-20

    A light-emitting device comprises a light-emitting member, which comprises two electrodes, at least two organic electroluminescent ("EL") materials disposed between the electrodes, a charge blocking material disposed between the electrodes, and at least one photoluminescent ("PL") material. The light-emitting member emits electromagnetic ("EM") radiation having a first spectrum in response to a voltage applied across the two electrodes. The PL material absorbs a portion of the EM radiation emitted by the light-emitting member and emits EM radiation having second spectrum different than the first spectrum. Each of the organic EL materials emits EM radiation having a wavelength range selected from the group consisting of blue and red wavelength ranges.

  16. Improved Limits on Gamma-Ray Burst Repetition from BATSE

    NASA Technical Reports Server (NTRS)

    Tegmark, Max; Hartmann, Dieter H.; Briggs, Michael S.; Hakkila, Jon; Meegan, Charles A.

    1996-01-01

    We tighten previous upper limits on gamma-ray burst repetition by analyzing the angular power spectrum of the BATSE 3B catalog of 1122 bursts. At 95% confidence, we find that no more than 2% of all observed bursts can be labeled as repeaters, even if no sources are observed to repeat more than once. If a fraction f of all observed bursts can be labeled as repeaters that are observed to burst upsilon times each, then all models with (upsilon - 1)f greater than or equal to 0.05 are ruled out at 99% confidence, as compared to the best previous 99% limit (upsilon - 1)f greater than or equal to 0.27. At 95% confidence, our new limit is (upsilon - 1)f greater than or equal to 0.02. Thus, even a cluster of six events from a single source would have caused excess power above that present in the 3B catalog. We conclude that the current BATSE data are consistent with no repetition of classical gamma-ray bursts and that any repeater model is severely constrained by the near-perfect isotropy of their angular distribution.

  17. Improved Limits on Gamma-Ray Burst Repetition from BATSE

    NASA Technical Reports Server (NTRS)

    Tegmark, Max; Hartmann, Dieter H.; Briggs, Michael S.; Meegan, Charles A.; Hakkila, Jon

    1996-01-01

    We tighten previous upper limits on gamma-ray burst repetition by analyzing the angular power spectrum of the BATSE 3B catalog of 1122 bursts. At 95% confidence, we find that no more than 2% of all observed bursts can be labeled as repeaters, even if no sources are observed to repeat more than once. If a fraction f of all observed bursts can be labeled as repeaters that are observed to burst nu times each, then all models with (nu - 1)f greater than or equal to 0.05 are ruled out at 99% confidence, as compared to the best previous 99% limit (nu - 1)f greater than or equal to 0.27. At 95% confidence, our new limit is (nu - 1)f greater than or equal to 0.02. Thus, even a cluster of six events from a single source would have caused excess power above that present in the 3B catalog. We conclude that the current BATSE data are consistent with no repetition of classical gamma-ray bursts and that any repeater model is severely constrained by the near-perfect isotropy of their angular dis- tribution.

  18. Development of an intraoperative gamma camera based on a 256-pixel mercuric iodide detector array

    NASA Astrophysics Data System (ADS)

    Patt, B. E.; Tornai, M. P.; Iwanczyk, J. S.; Levin, C. S.; Hoffman, E. J.

    1997-06-01

    A 256-element mercuric iodide (HgI/sub 2/) detector array has been developed which is intended for use as an intraoperative gamma camera (IOGC). The camera is specifically designed for use in imaging gamma-emitting radiopharmaceuticals (such as 99m-Tc labeled Sestamibi) incorporated into brain tumors in the intraoperative surgical environment. The system is intended to improve the success of tumor removal surgeries by allowing more complete removal of subclinical tumor cells without removal of excessive normal tissue. The use of HgI/sub 2/ detector arrays in this application facilitates construction of an imaging head that is very compact and has a high SNR. The detector is configured as a cross-grid array. Pixel dimensions are 1.25 mm squares separated by 0.25 mm. The overall dimension of the detector is 23.75 mm on a side. The detector thickness is 1 mm which corresponds to over 60% stopping at 140 keV. The array has good uniformity with average energy resolution of 5.2/spl plusmn/2.9% FWHM at 140 keV (best resolution was 1.9% FWHM). Response uniformity (/spl plusmn//spl sigma/) was 7.9%. A study utilizing realistic tumor phantoms (uptake ratio varied from 2:1 to 100:1) in background (1 mCi/l) was conducted. SNRs for the reasonably achievable uptake ratio of 50:1 were 5.61 /spl sigma/ with 1 cm of background depth ("normal tissue") and 2.74 /spl sigma/ with 4 cm of background for a 6.3 /spl mu/l tumor phantom (/spl sim/270 nCi at the time of the measurement).

  19. A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis.

    PubMed

    Xu, Jing; Xiong, Yan; Chen, Shiheng; Guan, Yafeng

    2008-07-15

    A light-emitting diode-induced fluorescence detector (LED-FD) for capillary electrophoresis was constructed and evaluated. A lamp LED with an enhanced emission spectrum and a band pass filter was used as the excitation light source. Refractive index matching fluid (RIMF) was used in the detection cell to reduce scattering light and the noise level. The limit of detection (LOD) for fluorescein was 1.5 nM (SNR=3). The system exhibited linear responses in the range of 1 x 10(-8) to 5 x 10(-6)M (R=0.999). Application of the lamp LED-FD for the analysis of FITC-labeled ephedra herb extract by capillary electrophoresis was demonstrated.

  20. A feasibility study of an integrated NIR/gamma/visible imaging system for endoscopic sentinel lymph node mapping.

    PubMed

    Kang, Han Gyu; Lee, Ho-Young; Kim, Kyeong Min; Song, Seong-Hyun; Hong, Gun Chul; Hong, Seong Jong

    2017-01-01

    The aim of this study is to integrate NIR, gamma, and visible imaging tools into a single endoscopic system to overcome the limitation of NIR using gamma imaging and to demonstrate the feasibility of endoscopic NIR/gamma/visible fusion imaging for sentinel lymph node (SLN) mapping with a small animal. The endoscopic NIR/gamma/visible imaging system consists of a tungsten pinhole collimator, a plastic focusing lens, a BGO crystal (11 × 11 × 2 mm 3 ), a fiber-optic taper (front = 11 × 11 mm 2 , end = 4 × 4 mm 2 ), a 122-cm long endoscopic fiber bundle, an NIR emission filter, a relay lens, and a CCD camera. A custom-made Derenzo-like phantom filled with a mixture of 99m Tc and indocyanine green (ICG) was used to assess the spatial resolution of the NIR and gamma images. The ICG fluorophore was excited using a light-emitting diode (LED) with an excitation filter (723-758 nm), and the emitted fluorescence photons were detected with an emission filter (780-820 nm) for a duration of 100 ms. Subsequently, the 99m Tc distribution in the phantom was imaged for 3 min. The feasibility of in vivo SLN mapping with a mouse was investigated by injecting a mixture of 99m Tc-antimony sulfur colloid (12 MBq) and ICG (0.1 mL) into the right paw of the mouse (C57/B6) subcutaneously. After one hour, NIR, gamma, and visible images were acquired sequentially. Subsequently, the dissected SLN was imaged in the same way as the in vivo SLN mapping. The NIR, gamma, and visible images of the Derenzo-like phantom can be obtained with the proposed endoscopic imaging system. The NIR/gamma/visible fusion image of the SLN showed a good correlation among the NIR, gamma, and visible images both for the in vivo and ex vivo imaging. We demonstrated the feasibility of the integrated NIR/gamma/visible imaging system using a single endoscopic fiber bundle. In future, we plan to investigate miniaturization of the endoscope head and simultaneous NIR/gamma/visible imaging with

  1. Z{gamma}{gamma}{gamma} {yields} 0 Processes in SANC

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bardin, D. Yu., E-mail: bardin@nu.jinr.ru; Kalinovskaya, L. V., E-mail: kalinov@nu.jinr.ru; Uglov, E. D., E-mail: corner@nu.jinr.ru

    2013-11-15

    We describe the analytic and numerical evaluation of the {gamma}{gamma} {yields} {gamma}Z process cross section and the Z {yields} {gamma}{gamma}{gamma} decay rate within the SANC system multi-channel approach at the one-loop accuracy level with all masses taken into account. The corresponding package for numeric calculations is presented. For checking of the results' correctness we make a comparison with the other independent calculations.

  2. Results from GROCSE I: A real-time search for gamma ray burst optical counterparts

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lee, B.; Akerlof, C.; Ables, E.

    The GROCSE I experiment (Gamma-Ray Optical Counterpart Search Experiment) is a rapid slewing wide field of view optical telescope at Lawrence Livermore National Laboratory which responds to triggers from the BATSE GRB data telemetry stream that have been processed and distributed by the BACODINE network. GROCSE 1 has been in continuous automated operation since January 1994. As of October 1995, sky images for 22 GRB triggers have been recorded, in some cases while the burst was still emitting gamma rays. The preliminary analysis of eight of these events are presented here. No optical counterparts have yet been detected. Limits formore » optical emission are given.« less

  3. Detection of CdSe quantum dot photoluminescence for security label on paper

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Isnaeni,, E-mail: isnaeni@lipi.go.id; Sugiarto, Iyon Titok; Bilqis, Ratu

    CdSe quantum dot has great potential in various applications especially for emitting devices. One example potential application of CdSe quantum dot is security label for anti-counterfeiting. In this work, we present a practical approach of security label on paper using one and two colors of colloidal CdSe quantum dot, which is used as stamping ink on various types of paper. Under ambient condition, quantum dot is almost invisible. The quantum dot security label can be revealed by detecting emission of quantum dot using photoluminescence and cnc machine. The recorded quantum dot emission intensity is then analyzed using home-made program tomore » reveal quantum dot pattern stamp having the word ’RAHASIA’. We found that security label using quantum dot works well on several types of paper. The quantum dot patterns can survive several days and further treatment is required to protect the quantum dot. Oxidation of quantum dot that occurred during this experiment reduced the emission intensity of quantum dot patterns.« less

  4. Multi-messenger Light Curves from Gamma-Ray Bursts in the Internal Shock Model

    NASA Astrophysics Data System (ADS)

    Bustamante, Mauricio; Heinze, Jonas; Murase, Kohta; Winter, Walter

    2017-03-01

    Gamma-ray bursts (GRBs) are promising as sources of neutrinos and cosmic rays. In the internal shock scenario, blobs of plasma emitted from a central engine collide within a relativistic jet and form shocks, leading to particle acceleration and emission. Motivated by present experimental constraints and sensitivities, we improve the predictions of particle emission by investigating time-dependent effects from multiple shocks. We produce synthetic light curves with different variability timescales that stem from properties of the central engine. For individual GRBs, qualitative conclusions about model parameters, neutrino production efficiency, and delays in high-energy gamma-rays can be deduced from inspection of the gamma-ray light curves. GRBs with fast time variability without additional prominent pulse structure tend to be efficient neutrino emitters, whereas GRBs with fast variability modulated by a broad pulse structure can be inefficient neutrino emitters and produce delayed high-energy gamma-ray signals. Our results can be applied to quantitative tests of the GRB origin of ultra-high-energy cosmic rays, and have the potential to impact current and future multi-messenger searches.

  5. Discovery of Giant Gamma-ray Bubbles in the Milky Way

    NASA Astrophysics Data System (ADS)

    Su, Meng

    Based on data from the Fermi Gamma-ray Space Telescope, we have discovered two gigantic gamma-ray emitting bubble structures in our Milky Way (known as the Fermi bubbles), extending ˜50 degrees above and below the Galactic center with a width of ˜40 degrees in longitude. The gamma-ray emission associated with these bubbles has a significantly harder spectrum (dN/dE ˜ E-2) than the inverse Compton emission from known cosmic ray electrons in the Galactic disk, or the gamma-rays produced by decay of pions from proton-ISM collisions. There is no significant difference in the spectrum or gamma-ray luminosity between the north and south bubbles. The bubbles are spatially correlated with the hard-spectrum microwave excess known as the WMAP haze; we also found features in the ROSAT soft X-ray maps at 1.5 -- 2 keV which line up with the edges of the bubbles. The Fermi bubbles are most likely created by some large episode of energy injection in the Galactic center, such as past accretion events onto the central massive black hole, or a nuclear starburst in the last ˜ 10 Myr. Study of the origin and evolution of the bubbles also has the potential to improve our understanding of recent energetic events in the inner Galaxy and the high-latitude cosmic ray population. Furthermore, we have recently identified a gamma-ray cocoon feature within the southern bubble, with a jet-like feature along the cocoon's axis of symmetry, and another directly opposite the Galactic center in the north. If confirmed, these jets are the first resolved gamma-ray jets ever seen.

  6. Carbon-11 and Fluorine-18 Labeled Amino Acid Tracers for Positron Emission Tomography Imaging of Tumors

    NASA Astrophysics Data System (ADS)

    Sun, Aixia; Liu, Xiang; Tang, Ganghua

    2017-12-01

    Tumor cells have an increased nutritional demand for amino acids(AAs) to satisfy their rapid proliferation. Positron-emitting nuclide labeled AAs are interesting probes and are of great importance for imaging tumors using positron emission tomography (PET). Carbon-11 and fluorine-18 labeled AAs include the [1-11C] amino acids, labeling alpha-C- amino acids, the branched-chain of amino acids and N-substituted carbon-11 labeled amino acids. These tracers target protein synthesis or amino acid(AA) transport, and their uptake mechanism mainly involves AA transport. AA PET tracers have been widely used in clinical settings to image brain tumors, neuroendocrine tumors, prostate cancer, breast cancer, non–small cell lung cancer (NSCLC) and hepatocellular carcinoma. This review focuses on the fundamental concepts and the uptake mechanism of AAs, AA PET tracers and their clinical applications.

  7. A phoswich detector for simultaneous alpha-gamma spectroscopy

    NASA Astrophysics Data System (ADS)

    Moghadam, S. Rajabi; Feghhi, S. A. H.; Safari, M. J.

    2015-11-01

    Phoswich detectors are of value for radiation spectroscopy, especially in cases where a low-cost solution for a mixed radiation field is desired. Meanwhile, simultaneous spectroscopy of alpha particles and gamma-rays has many applications in quantification and distinguishing the alpha-emitting radionuclides which usually occur in the analysis of environmental solid samples. Here, we have developed a system for detection of radioactive actinides (e.g., 241Am) based on the alpha-gamma coincidence technique. The underlying concept, is to assemble two appropriately selected scintillators (i.e., a fast and a slow one) together with a discriminating unit for analysis of their data. Detailed Monte Carlo simulation procedure has been developed using the GEANT4 toolkit to design and find enough knowledge about the response of the system in the studied radiation field. Various comparisons were made between experimental and simulation data which showed appropriate agreement between them. The calibration was performed and the MDA was estimated as 60 mBq for the phoswich system.

  8. Observation of Doppler broadening in beta-delayed proton-gamma decay

    NASA Astrophysics Data System (ADS)

    Schwartz, Sarah

    The Doppler broadening of gamma-ray peaks due to nuclear recoil from beta-delayed nucleon emission can be used to measure the energies of the nucleons. The purpose of this Thesis is to test and apply this Doppler broadening method using gamma-ray peaks from the 26P(betapgamma) 25Al decay sequence. A fast beam of 26P was implanted into a planar Ge detector, which was used as a 26P beta-decay trigger. The SeGA array of high-purity Ge detectors was used to detect gamma rays from the 26P(betapgamma)25Al decay sequence. Radiative Doppler broadening in beta-delayed proton-gamma decay was observed for the first time. The Doppler broadening analysis method was verified using the 1613 keV gamma-ray line for which the proton energies were previously known. The 1776 keV gamma ray de-exciting the 2720 keV 25Al level was observed in 26P(betapgamma) 25Al decay for the first time and used to determine that the center-of-mass energy of the proton emission feeding the 2720-keV level is 5.1 +/- 1.0 (stat.) +/- 0.6 (syst.) MeV, corresponding to a 26Si excitation energy of 13.3 +/- 1.0 (stat.) +/- 0.7 (syst.) MeV for the proton-emitting level. The Doppler broadening method has been demonstrated to provide practical measurements of the energies for beta-delayed nucleon emissions populating excited states of nuclear recoils at least as heavy as A = 25.

  9. Acute effects of sodium valproate and gamma-vinyl GABA on regional amino acid metabolism in the rat brain: incorporation of 2-[14C]glucose into amino acids.

    PubMed

    Chapman, A G; Riley, K; Evans, M C; Meldrum, B S

    1982-09-01

    Amino acid concentrations have been determined in rat brain regions (cortex, striatum, cerebellum, and hippocampus) by HPLC after administration of acute anticonvulsant doses of sodium valproate (400 mg/kg, i.p.) and gamma-vinyl-GABA (1 g/kg, i.p.). After valproate administration the GABA level increases only in the cortex; aspartic acid concentration decreases in the cortex and hippocampus, and glutamic acid decreases in the hippocampus and striatum and increases in the cortex and cerebellum. There are no changes in the concentrations of glutamine, taurine, glycine, serine, and alanine following valproate administration. Only the GABA level increases in all the regions after gamma-vinyl-GABA administration. Cortical analyses 2, 4 and 10 minutes after pulse labeling with 2-[14C]glucose, i.v., show no change in the rate of cortical glucose utilization in the valproate treated group. The rate of labeling of glutamic acid is also unchanged, but the rate of labeling of GABA is reduced following valproate administration. After gamma-vinyl-GABA administration there is no change in the rate of labeling of GABA. These biochemical findings can be interpreted in terms of a primary anticonvulsant action of valproate on membrane receptors with secondary effects on the metabolism of amino acid neurotransmitters. This contrasts with the primary action of gamma-vinyl-GABA on GABA-transaminase activity.

  10. Measurement of angularly dependent spectra of betatron gamma-rays from a laser plasma accelerator with quadrant-sectored range filters

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jeon, Jong Ho, E-mail: jhjeon07@ibs.re.kr; Nakajima, Kazuhisa, E-mail: naka115@dia-net.ne.jp; Rhee, Yong Joo

    Measurement of angularly dependent spectra of betatron gamma-rays radiated by GeV electron beams from laser wakefield accelerators (LWFAs) are presented. The angle-resolved spectrum of betatron radiation was deconvolved from the position dependent data measured for a single laser shot with a broadband gamma-ray spectrometer comprising four-quadrant sectored range filters and an unfolding algorithm, based on the Monte Carlo code GEANT4. The unfolded gamma-ray spectra in the photon energy range of 0.1–10 MeV revealed an approximately isotropic angular dependence of the peak photon energy and photon energy-integrated fluence. As expected by the analysis of betatron radiation from LWFAs, the results indicate thatmore » unpolarized gamma-rays are emitted by electrons undergoing betatron motion in isotropically distributed orbit planes.« less

  11. A photoaffinity scan maps regions of the p85 SH2 domain involved in phosphoprotein binding.

    PubMed

    Williams, K P; Shoelson, S E

    1993-03-15

    Src homology 2 (SH2) domains are modular phosphotyrosine binding pockets found within a wide variety of cytoplasmic signaling molecules. Here we develop a new approach to analyzing protein-protein interfaces termed photoaffinity scanning, and apply the method to map regions of the phosphatidylinositol 3-kinase p85 SH2 domain that participate in phospho-protein binding. Each residue except phosphotyrosine (pY) within a tightly binding, IRS-1-derived phosphopeptide (GNGDpYMPMSPKS) was substituted with the photoactive amino acid, benzoylphenylalanine (Bpa). Whereas most substitutions had little effect on binding affinity, Bpa substitution of either Met (+1 and +3 with respect to pY) reduced affinity 50-100-fold to confirm their importance in the pYMXM recognition motif. In three cases photolysis of SH2 domain/Bpa phosphopeptide complexes led to cross-linking of > 50% of the SH2 domain; cross-link positions were identified by microsequence, amino acid composition, and electrospray mass spectrometric analyses. Bpa-1 cross-links within alpha-helix I, whereas Bpa+1 and Bpa+4 cross-link the SH2 domain within the flexible loop C-terminal to alpha-helix II. Moreover, cross-linking at any position prevents SH2 domain cleavage at a trypsin-sensitive site within the flexible loop between beta-strands 1 and 2. Therefore, at least three distinct SH2 regions in addition to the beta-sheet participate in phosphoprotein binding; the loop cross-linked by phosphopeptide residues C-terminal to pY appears to confer specificity to the phosphoprotein/SH2 domain interaction.

  12. Ablation of phosphoinositide 3-kinase-gamma reduces the severity of acute pancreatitis.

    PubMed

    Lupia, Enrico; Goffi, Alberto; De Giuli, Paolo; Azzolino, Ornella; Bosco, Ornella; Patrucco, Enrico; Vivaldo, Maria Cristina; Ricca, Marco; Wymann, Matthias P; Hirsch, Emilio; Montrucchio, Giuseppe; Emanuelli, Giorgio

    2004-12-01

    In pancreatic acini, the G-protein-activated phosphoinositide 3-kinase-gamma (PI3K gamma) regulates several key pathological responses to cholecystokinin hyperstimulation in vitro. Thus, using mice lacking PI3K gamma, we studied the function of this enzyme in vivo in two different models of acute pancreatitis. The disease was induced by supramaximal concentrations of cerulein and by feeding mice a choline-deficient/ethionine-supplemented diet. Although the secretive function of isolated pancreatic acini was identical in mutant and control samples, in both models, genetic ablation of PI3K gamma significantly reduced the extent of acinar cell injury/necrosis. In agreement with a protective role of apoptosis in pancreatitis, PI3K gamma-deficient pancreata showed an increased number of apoptotic acinar cells, as determined by terminal dUTP nick-end labeling and caspase-3 activity. In addition, neutrophil infiltration within the pancreatic tissue was also reduced, suggesting a dual action of PI3K gamma, both in the triggering events within acinar cells and in the subsequent neutrophil recruitment and activation. Finally, the lethality of the choline-deficient/ethionine-supplemented diet-induced pancreatitis was significantly reduced in mice lacking PI3K gamma. Our results thus suggest that inhibition of PI3K gamma may be of therapeutic value in acute pancreatitis.

  13. The development of the gamma-ray Cherenkov telescope at the South Pole

    NASA Astrophysics Data System (ADS)

    Barbagli, G.; Castellini, G.; Landi, G.; Morse, R.; Tasselli, P. L.; Tilav, S.

    1993-05-01

    An atmospheric Cherenkov telescope (ACT) designated GASP (Gamma Astronomy at the South Pole) has been installed near the SPASE (South Pole Air Shower Experiment) scintillator array at the South Pole Observing Facility. During the austral summer 1992, it was aimed at objects such as PKS 0537-441 and PSR 1706-44, simultaneously with the Egret detector on the Compton Gamma Ray Observatory (CGRO). Recently observed radio silent quasars that emit gamma rays with enormous intensity may account for the origin of cosmic ray flux at energies that exceed 1016 eV. Other galactic sources like Cygnus X-3 are believed to produce the UHE cosmic gamma flux. In addition to SPASE and GASP, a group of particle detectors (AMANDA, ACA, POOL, MICE) are currently being tested. The above instrumentation will constitute the South Pole Observatory Facility. The members of the GASP collaboration are as follows: Firenze: G. Barbagli G. Castellini, G. Landi, P.L. Tasselli; Purdue: J. Gaidos, F. Loeffler, G. Sembroski; C. Wilson; Smithsonian: K. Harris, M.A. Lawrence, T.C. Weekes; Wisconsin K. Engel, F. Halzen R. Morse, P. Surrey, S. Tilav

  14. Field-deployable gamma-radiation detectors for DHS use

    NASA Astrophysics Data System (ADS)

    Mukhopadhyay, Sanjoy

    2007-09-01

    Recently, the Department of Homeland Security (DHS) has integrated all nuclear detection research, development, testing, evaluation, acquisition, and operational support into a single office: the Domestic Nuclear Detection Office (DNDO). The DNDO has specific requirements set for all commercial off-the-shelf and government off-the-shelf radiation detection equipment and data acquisition systems. This article would investigate several recent developments in field deployable gamma radiation detectors that are attempting to meet the DNDO specifications. Commercially available, transportable, handheld radio isotope identification devices (RIID) are inadequate for DHS' requirements in terms of sensitivity, resolution, response time, and reach-back capability. The leading commercial vendor manufacturing handheld gamma spectrometer in the United States is Thermo Electron Corporation. Thermo Electron's identiFINDER TM, which primarily uses sodium iodide crystals (3.18 x 2.54cm cylinders) as gamma detectors, has a Full-Width-at-Half-Maximum energy resolution of 7 percent at 662 keV. Thermo Electron has just recently come up with a reach-back capability patented as RadReachBack TM that enables emergency personnel to obtain real-time technical analysis of radiation samples they find in the field1. The current project has the goal to build a prototype handheld gamma spectrometer, equipped with a digital camera and an embedded cell phone to be used as an RIID with higher sensitivity, better resolution, and faster response time (able to detect the presence of gamma-emitting radio isotopes within 5 seconds of approach), which will make it useful as a field deployable tool. The handheld equipment continuously monitors the ambient gamma radiation, and, if it comes across any radiation anomalies with higher than normal gamma gross counts, it sets an alarm condition. When a substantial alarm level is reached, the system automatically triggers the saving of relevant spectral data and

  15. Evaluation of the applicability of the SeHCAT test in the investigation of patients with diarrhoea.

    PubMed

    Orholm, M; Pedersen, J O; Arnfred, T; Rødbro, P; Thaysen, E H

    1988-01-01

    For the assessment of ileal bile acid conservation the retention of orally administered 75Se-23-selena-25-homotaurocholic acid (SeHCAT), a gamma-ray-emitting radioisotope-labelled synthetic bile acid, was measured by an uncollimated abdominal gamma camera in 89 patients with various gastrointestinal disorders and in 20 persons without gastrointestinal complaints. A significant differences in retention was observed between patients with and without previously detected ileal disease. However, it was not possible by use of the test to differentiate between the various types of diarrhoea. Hence the test is not recommended in the routine investigation of patients with diarrhoea.

  16. Photoaffinity labeling and partial purification of the beta cell sulfonylurea receptor using a novel, biologically active glyburide analog

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Aguilar-Bryan, L.; Nelson, D.A.; Vu, Q.A.

    1990-05-15

    An iodinated analog of the sulfonylurea, glyburide, has been synthesized which can be labeled to high specific activity and used to photolabel the sulfonylurea receptor. 5-Iodo-2-hydroxy-glyburide, has an iodo group replacing the chlorine at position 5 and a methoxy residue replacing the hydroxy group at position 2 on the benzamido ring. This analog retains biologic activity stimulating insulin secretion from a hamster beta cell line (HIT cells) at the same ED50 (0.4 nM) as glyburide. Scatchard analysis demonstrated high and low affinity binding sites on HIT cell membranes (Kd values of 0.36 nM and 277 nM and Bmax values ofmore » 1.6 and 100 pmol/mg of membrane protein, respectively). Competitive binding assays with unlabeled glyburide or 5-iodo-2-hydroxyglyburide yield Ki values of 0.5 and 1.0 nM, respectively. The analog can be covalently linked by ultraviolet irradiation to a membrane protein of Mr = 140,000. The photolabeling is completely blocked by unlabeled glyburide or the analog. Two other species of Mr = 65,000 and 43,000 are also photolabeled; these may be the low affinity sites. After photolabeling, the receptor has been purified partially by chromatographic procedures and is suitable for obtaining peptide sequence. The 140,000 molecular weight protein is identified as the sulfonylurea receptor since its binding constant, 0.36 nM, is closely correlated with its ability to stimulate insulin secretion (ED50 congruent to 0.4 nM).« less

  17. The Z {yields} cc-bar {yields} {gamma}{gamma}*, Z {yields} bb-bar {yields} {gamma}{gamma}* triangle diagrams and the Z {yields} {gamma}{psi}, Z {yields} {gamma}Y decays

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Achasov, N. N., E-mail: achasov@math.nsc.ru

    2011-03-15

    The approach to the Z {yields} {gamma}{psi} and Z {yields} {gamma}Y decay study is presented in detail, based on the sum rules for the Z {yields} cc-bar {yields} {gamma}{gamma}* and Z {yields} bb-bar {yields} {gamma}{gamma}* amplitudes and their derivatives. The branching ratios of the Z {yields} {gamma}{psi} and Z {yields} {gamma}Y decays are calculated for different hypotheses on saturation of the sum rules. The lower bounds of {Sigma}{sub {psi}} BR(Z {yields} {gamma}{psi}) = 1.95 Multiplication-Sign 10{sup -7} and {Sigma}{sub {upsilon}} BR(Z {yields} {gamma}Y) = 7.23 Multiplication-Sign 10{sup -7} are found. Deviations from the lower bounds are discussed, including the possibilitymore » of BR(Z {yields} {gamma}J/{psi}(1S)) {approx} BR(Z {yields} {gamma}Y(1S)) {approx} 10{sup -6}, that could be probably measured in LHC. The angular distributions in the Z {yields} {gamma}{psi} and Z {yields} {gamma}Y decays are also calculated.« less

  18. A flexible top-emitting organic light-emitting diode on steel foil

    NASA Astrophysics Data System (ADS)

    Xie, Zhiyuan; Hung, Liang-Sun; Zhu, Furong

    2003-11-01

    An efficient flexible top-emitting organic light-emitting diode (FTOLED) was developed on a thin steel foil. The FTOLED was constructed on the spin-on-glass (SOG)-coated steel substrate with an organic stack of NPB/Alq 3 sandwiched by a highly reflective Ag anode and a semitransparent Sm cathode. An ultrathin plasma-polymerized hydrocarbon film (CF X) was interposed between the Ag anode and the NPB layer to enhance hole-injection, and an additional Alq 3 layer was overlaid on the Sm cathode to increase light output. The FTOLED showed a peak efficiency of 4.4 cd/A higher than 3.7 cd/A of a convention NPB/Alq 3-based bottom-emitting OLED.

  19. e-ASTROGAM mission: a major step forward for gamma-ray polarimetry

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tatischeff, Vincent; De Angelis, Alessandro; Gouiffes, Christian

    e-ASTROGAM is a gamma-ray space mission proposed for the fifth medium-size mission (M5) of the European Space Agency. It is dedicated to the study of the nonthermal universe in the photon energy range from ~ 0.15 MeV to 3 GeV with unprecedented sensitivity and angular and energy resolution, together with a ground-breaking capability for gamma-ray polarimetric measurements over its entire bandwidth. We discuss here the main polarization results expected at low energies, between 150 keV and 5 MeV, using Compton interactions in the e-ASTROGAM instrument, from observations of active galactic nuclei, gamma-ray bursts, microquasars, and the Crab Pulsar and Nebula.more » The anticipated performance of the proposed observatory for polarimetry is illustrated by simulations of the polarization signals expected from various sources. We show that polarimetric analyses with e-ASTROGAM should provide definitive insight into the geometry, magnetization, and content of the high-energy plasmas found in the emitting sources, as well as on the processes of radiation of these plasmas.« less

  20. e-ASTROGAM mission: a major step forward for gamma-ray polarimetry

    DOE PAGES

    Tatischeff, Vincent; De Angelis, Alessandro; Gouiffes, Christian; ...

    2017-11-28

    e-ASTROGAM is a gamma-ray space mission proposed for the fifth medium-size mission (M5) of the European Space Agency. It is dedicated to the study of the nonthermal universe in the photon energy range from ~ 0.15 MeV to 3 GeV with unprecedented sensitivity and angular and energy resolution, together with a ground-breaking capability for gamma-ray polarimetric measurements over its entire bandwidth. We discuss here the main polarization results expected at low energies, between 150 keV and 5 MeV, using Compton interactions in the e-ASTROGAM instrument, from observations of active galactic nuclei, gamma-ray bursts, microquasars, and the Crab Pulsar and Nebula.more » The anticipated performance of the proposed observatory for polarimetry is illustrated by simulations of the polarization signals expected from various sources. We show that polarimetric analyses with e-ASTROGAM should provide definitive insight into the geometry, magnetization, and content of the high-energy plasmas found in the emitting sources, as well as on the processes of radiation of these plasmas.« less

  1. An Unusual Supernova in the Error Box of the Gamma-Ray Burst of 25 April 1998

    NASA Technical Reports Server (NTRS)

    Galama , T. J.; Vreeswijk, P. M.; vanParadijs, J.; Kouveliotou, C.; Augusteijn, T.; Boehnhardt, H.; Brewer, J. P.; Doublier, V.; Gonzalez, J.-F.; Leibundgut, B.; hide

    1999-01-01

    The discovery of afterglows associated with gamma-ray bursts at X-ray, optical and radio wavelengths and the measurement of the redshifts of some of these events has established that gamma-ray bursts lie at extreme distances, making them the most powerful photon-emitters known in the Universe. Here we report the discovery of transient optical emission in the error box of the gamma-ray burst GRB980425, the light curve of which was very different from that of previous optical afterglows associated with gamma-ray bursts. The optical transient is located in a spiral arm of the galaxy ESO 184-GS2, which has a redshift velocity of only 2,550 km/ s. Its optical spectrum and location indicate that it is a very luminous supernova, which has been identified as SN1998bw. If this supernova and GRB980425 are indeed associated, the energy radiated in gamma-rays is at least four orders of magnitude less than in other gamma-ray bursts, although its appearance was otherwise unremarkable: this indicates that very different mechanisms can give rise to gamma-ray bursts. But independent of this association, the supernova is itself unusual, exhibiting an unusual light curve at radio wavelengths that requires that the gas emitting the radio photons be expanding relativistically.

  2. Gamma-ray detection efficiency of the microchannel plate installed as an ion detector in the low energy particle instrument onboard the GEOTAIL satellite.

    PubMed

    Tanaka, Y T; Yoshikawa, I; Yoshioka, K; Terasawa, T; Saito, Y; Mukai, T

    2007-03-01

    A microchannel plate (MCP) assembly has been used as an ion detector in the low energy particle (LEP) instrument onboard the magnetospheric satellite GEOTAIL. Recently the MCP assembly has detected gamma rays emitted from an astronomical object and has been shown to provide unique information of gamma rays if they are intense enough. However, the detection efficiency for gamma rays was not measured before launch, and therefore we could not analyze the LEP data quantitatively. In this article, we report the gamma-ray detection efficiency of the MCP assembly. The measured efficiencies are 1.29%+/-0.71% and 0.21%+/-0.14% for normal incidence 60 and 662 keV gamma rays, respectively. The incident angle dependence is also presented. Our calibration is crucial to study high energy astrophysical phenomena by using the LEP.

  3. The Imprint of the Extragalactic Background Light in the Gamma-Ray Spectra of Blazars

    NASA Technical Reports Server (NTRS)

    Ackermann, M.; Ajello, M.; Allafort, A.; Schady, P.; Baldini, L.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Bellazzini, R; Blandford, R. D.; hide

    2012-01-01

    The light emitted by stars and accreting compact objects through the history of the universe is encoded in the intensity of the extragalactic background light (EBL). Knowledge of the EBL isimportant to understand the nature of star formation and galaxy evolution, but direct measurements of the EBL are limited by galactic and other foreground emissions. Here, we report an absorption feature seen in the combined spectra of a sample of gamma-ray blazars out to a redshift of z approx. 1.6. This feature is caused by attenuation of gamma rays by the EBL at optical to ultraviolet frequencies and allowed us to measure the EBL flux density in this frequency band.

  4. Relative risk analysis in regulating the use of radiation-emitting medical devices. A preliminary application

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jones, E.D.; Banks, W.W.; Altenbach, T.J.

    1995-09-01

    This report describes a preliminary application of an analysis approach for assessing relative risks in the use of radiation- emitting medical devices. Results are presented on human-initiated actions and failure modes that are most likely to occur in the use of the Gamma Knife, a gamma irradiation therapy device. This effort represents an initial step in a US Nuclear Regulatory Commission (NRC) plan to evaluate the potential role of risk analysis in regulating the use of nuclear medical devices. For this preliminary application of risk assessment, the focus was to develop a basic process using existing techniques for identifying themore » most likely risk contributors and their relative importance. The approach taken developed relative risk rankings and profiles that incorporated the type and quality of data available and could present results in an easily understood form. This work was performed by the Lawrence Livermore National Laboratory for the NRC.« less

  5. The role of extracellular calcium in corticotropin-stimulated steroidogenesis.

    PubMed

    Cheitlin, R; Buckley, D I; Ramachandran, J

    1985-05-10

    The role of extracellular Ca2+ in the binding of corticotropin (ACTH) to adrenocortical cell receptors as well as in the post-binding events involved in steroidogenesis were investigated. Binding studies using [125I-Tyr23,Phe2,Nle4]ACTH (1-38) peptide showed that extracellular Ca2+ is essential not only for the interaction of ACTH with its receptor, but also for continued occupancy of the receptor. In view of the requirement of Ca2+ for binding the hormone to the receptor, the role of Ca2+ in post-receptor events was investigated by covalently attaching the hormone to its receptor by photoaffinity labeling in the presence of Ca2+. Persistent activation of steroidogenesis induced by photoaffinity labeling in the presence of Ca2+ was depressed when cells were incubated in medium containing EGTA but was unaffected when the cells were merely washed and incubated in Ca2+-free medium. In the presence of EGTA, 8-Br-cAMP partially restored persistent activation of steroidogenesis. The concentration of extracellular Ca2+ required for restoring steroidogenesis was 10-fold lower than the concentration of Ca2+ needed for optimal binding of ACTH to its receptor. These results suggest that the primary role of extracellular Ca2+ in the action of ACTH is to facilitate the association of the hormone with its receptor.

  6. Identification and Cloning of Centaurin-α

    PubMed Central

    Hammonds-Odie, Latanya P.; Jackson, Trevor R.; Profit, Adam A.; Blader, Ira J.; Turck, Christoph W.; Prestwich, Glenn D.; Theibert, Anne B.

    2015-01-01

    Using an affinity resin and photoaffinity label based on phospholipid analogs of inositol 1,3,4,5-tetrakisphosphate (InsP4), we have isolated, characterized, and cloned a 46-kDa protein from rat brain, which we have named centaurin-α. Binding specificity was determined using displacement of 1-O-[3H](3-[4-benzoyldihydrocinnamidyl]propyl)-InsP4 photoaffinity labeling. Centaurin-α displayed highest affinity for phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) (IC50 = 120 nm), whereas InsP4, PtdInsP2, and InsP3 bound with 5-, 12-, and >50-fold lower affinity, respectively. Screening a rat brain cDNA library with a polymerase chain reaction product, generated using partial amino acid sequence from tryptic peptides, yielded a full-length clone. The 2,450-base pair cDNA contained an open reading frame (ORF) encoding a novel protein of 419 amino acids. Northern analysis revealed a 2.5-kilobase transcript that is highly expressed in brain. The deduced sequence contains a novel putative zinc finger motif, 10 ankyrin-like repeats, and shows homology to recently identified yeast and mammalian Arf GTPase-activating proteins. Given the specificity of binding and enrichment in brain, centaurin-α is a candidate PtdInsP3 receptor that may link the activation of phosphoinositide 3-kinase to downstream responses in the brain. PMID:8702546

  7. Volatile pollutants emitted from selected liquid household products.

    PubMed

    Kwon, Ki-Dong; Jo, Wan-Kuen; Lim, Ho-Jin; Jeong, Woo-Sik

    2008-09-01

    classes or product categories, and it was different from that reported in other studies abroad, although certain target chemicals were identified in both studies. This finding supports an assertion that chemical components emitted from household products may be different in different products and with different manufacturers. The chlorinated pollutants identified in the present study have not been reported to be components of cleaning products in papers published since the early 1990s. Limonene was identified as having the highest occurrence in the household products in the present study, although it was not detected in any of 67 household products sold in the U.S. The emission composition of selected household products was successfully examined by purge-and-trap analysis. Along with other exposure information such as use pattern of household products and the indoor climate, this composition data can be used to estimate personal exposure levels of building occupants. This exposure data can be employed to link environmental exposure to health risk. It is noteworthy that many liquid household products sold in Korea emitted several toxic aromatic and chlorinated organic compounds. Moreover, the current finding suggests that product types and manufacturers should be considered, when evaluating building occupants' exposure to chemical components emitted from household products. The current findings can provide valuable information for the semiquantitative estimation of the population inhalation exposure to these compounds in indoor environments and for the selection of safer household products. However, although the chemical composition is known, the emissions of household products might include compounds formed during the use of the product or compounds not identified as ingredients by this study. Accordingly, further studies are required, and testing must be done to determine the actual composition being emitted. Similar to eco-labeling of shampoos, shower gels, and foam

  8. Multiwavelength observations of a VHE gamma-ray flare from PKS 1510-089 in 2015

    NASA Astrophysics Data System (ADS)

    Ahnen, M. L.; Ansoldi, S.; Antonelli, L. A.; Arcaro, C.; Babić, A.; Banerjee, B.; Bangale, P.; Barres de Almeida, U.; Barrio, J. A.; Bednarek, W.; Bernardini, E.; Berti, A.; Biasuzzi, B.; Biland, A.; Blanch, O.; Bonnefoy, S.; Bonnoli, G.; Borracci, F.; Bretz, T.; Carosi, R.; Carosi, A.; Chatterjee, A.; Colin, P.; Colombo, E.; Contreras, J. L.; Cortina, J.; Covino, S.; Cumani, P.; Da Vela, P.; Dazzi, F.; De Angelis, A.; De Lotto, B.; de Oña Wilhelmi, E.; Di Pierro, F.; Doert, M.; Domínguez, A.; Dominis Prester, D.; Dorner, D.; Doro, M.; Einecke, S.; Eisenacher Glawion, D.; Elsaesser, D.; Engelkemeier, M.; Fallah Ramazani, V.; Fernández-Barral, A.; Fidalgo, D.; Fonseca, M. V.; Font, L.; Fruck, C.; Galindo, D.; García López, R. J.; Garczarczyk, M.; Gaug, M.; Giammaria, P.; Godinović, N.; Gora, D.; Guberman, D.; Hadasch, D.; Hahn, A.; Hassan, T.; Hayashida, M.; Herrera, J.; Hose, J.; Hrupec, D.; Hughes, G.; Ishio, K.; Konno, Y.; Kubo, H.; Kushida, J.; Kuveždić, D.; Lelas, D.; Lindfors, E.; Lombardi, S.; Longo, F.; López, M.; Majumdar, P.; Makariev, M.; Maneva, G.; Manganaro, M.; Mannheim, K.; Maraschi, L.; Mariotti, M.; Martínez, M.; Mazin, D.; Menzel, U.; Mirzoyan, R.; Moralejo, A.; Moretti, E.; Nakajima, D.; Neustroev, V.; Niedzwiecki, A.; Nievas Rosillo, M.; Nilsson, K.; Nishijima, K.; Noda, K.; Nogués, L.; Paiano, S.; Palacio, J.; Palatiello, M.; Paneque, D.; Paoletti, R.; Paredes, J. M.; Paredes-Fortuny, X.; Pedaletti, G.; Peresano, M.; Perri, L.; Persic, M.; Poutanen, J.; Prada Moroni, P. G.; Prandini, E.; Puljak, I.; Garcia, J. R.; Reichardt, I.; Rhode, W.; Ribó, M.; Rico, J.; Saito, T.; Satalecka, K.; Schroeder, S.; Schweizer, T.; Shore, S. N.; Sillanpää, A.; Sitarek, J.; Šnidarić, I.; Sobczynska, D.; Stamerra, A.; Strzys, M.; Surić, T.; Takalo, L.; Tavecchio, F.; Temnikov, P.; Terzić, T.; Tescaro, D.; Teshima, M.; Torres, D. F.; Torres-Albà, N.; Toyama, T.; Treves, A.; Vanzo, G.; Vazquez Acosta, M.; Vovk, I.; Ward, J. E.; Will, M.; Wu, M. H.; Zarić, D.; Desiante, R.; Becerra González, J.; D'Ammando, F.; Larsson, S.; Raiteri, C. M.; Reinthal, R.; Lähteenmäki, A.; Järvelä, E.; Tornikoski, M.; Ramakrishnan, V.; Jorstad, S. G.; Marscher, A. P.; Bala, V.; MacDonald, N. R.; Kaur, N.; Sameer; Baliyan, K.; Acosta-Pulido, J. A.; Lazaro, C.; Martí-nez-Lombilla, C.; Grinon-Marin, A. B.; Pastor Yabar, A.; Protasio, C.; Carnerero, M. I.; Jermak, H.; Steele, I. A.; Larionov, V. M.; Borman, G. A.; Grishina, T. S.

    2017-07-01

    Context. PKS 1510-089 is one of only a few flat spectrum radio quasars detected in the very-high-energy (VHE, > 100 GeV) gamma-ray band. Aims: We study the broadband spectral and temporal properties of the PKS 1510-089 emission during a high gamma-ray state. Methods: We performed VHE gamma-ray observations of PKS 1510-089 with the Major Atmospheric Gamma Imaging Cherenkov (MAGIC) telescopes during a long, high gamma-ray state in May 2015. In order to perform broadband modeling of the source, we have also gathered contemporaneous multiwavelength data in radio, IR, optical photometry and polarization, UV, X-ray, and GeV gamma-ray ranges. We construct a broadband spectral energy distribution (SED) in two periods, selected according to VHE gamma-ray state. Results: PKS 1510-089 was detected by MAGIC during a few day-long observations performed in the middle of a long, high optical and gamma-ray state, showing for the first time a significant VHE gamma-ray variability. Similarly to the optical and gamma-ray high state of the source detected in 2012, it was accompanied by a rotation of the optical polarization angle and the emission of a new jet component observed in radio. However, owing to large uncertainty on the knot separation time, the association with the VHE gamma-ray emission cannot be firmly established. The spectral shape in the VHE band during the flare is similar to those obtained during previous measurements of the source. The observed flux variability sets constraints for the first time on the size of the region from which VHE gamma rays are emitted. We model the broadband SED in the framework of the external Compton scenario and discuss the possible emission site in view of multiwavelength data and alternative emission models.

  9. Recombination zone in white organic light emitting diodes with blue and orange emitting layers

    NASA Astrophysics Data System (ADS)

    Tsuboi, Taiju; Kishimoto, Tadashi; Wako, Kazuhiro; Matsuda, Kuniharu; Iguchi, Hirofumi

    2012-10-01

    White fluorescent OLED devices with a 10 nm thick blue-emitting layer and a 31 nm thick orange-emitting layer have been fabricated, where the blue-emitting layer is stacked on a hole transport layer. An interlayer was inserted between the two emitting layers. The thickness of the interlayer was changed among 0.3, 0.4, and 1.0 nm. White emission with CIE coordinates close to (0.33, 0.33) was observed from all the OLEDs. OLED with 0.3 nm thick interlayer gives the highest maximum luminous efficiency (11 cd/A), power efficiency (9 lm/W), and external quantum efficiency (5.02%). The external quantum efficiency becomes low with increasing the interlayer thickness from 0 nm to 1.0 nm. When the location of the blue- and orange-emitting layers is reversed, white emission was not obtained because of too weak blue emission. It is suggested that the electron-hole recombination zone decreases nearly exponentially with a distance from the hole transport layer.

  10. Category labels versus feature labels: category labels polarize inferential predictions.

    PubMed

    Yamauchi, Takashi; Yu, Na-Yung

    2008-04-01

    What makes category labels different from feature labels in predictive inference? This study suggests that category labels tend to make inductive reasoning polarized and homogeneous. In two experiments, participants were shown two schematic pictures of insects side by side and predicted the value of a hidden feature of one insect on the basis of the other insect. Arbitrary verbal labels were shown above the two pictures, and the meanings of the labels were manipulated in the instructions. In one condition, the labels represented the category membership of the insects, and in the other conditions, the same labels represented attributes of the insects. When the labels represented category membership, participants' responses became substantially polarized and homogeneous, indicating that the mere reference to category membership can modify reasoning processes.

  11. Fermi Bubble: Giant Gamma-Ray Bubbles in the Milky Way

    NASA Astrophysics Data System (ADS)

    Su, Meng

    Data from the Fermi-LAT reveal two gigantic gamma-ray emitting bubble structures (known as the Fermibubbles), extending˜50° above and below the Galactic center symmetric about the Galactic plane, with a width of˜40∘ in longitude. The gamma-ray emission associated with these bubbles has a significantly harder spectrum ({dN}/{dE} ˜ {E}^{-2}) than the inverse Compton emission from known cosmic ray electrons in the Galactic disk, or the gamma-rays produced by decay of pions from proton-ISM collisions. The bubbles are spatially correlated with the hard-spectrum microwave excess known as the WMAPhaze; the edges of the bubbles also line up with features in the ROSATsoft X-ray maps at 1.5-2keV. The Fermibubble is most likely created by some large episode of energy injection in the Galactic center, such as past accretion events onto the central massive black hole, or a nuclear starburst in the last˜10Myr. Study of the origin and evolution of the bubbles also has the potential to improve our understanding of recent energetic events in the inner Galaxy and the high-latitude cosmic ray population.

  12. A method for the 32P labeling of peptides or peptide nucleic acid oligomers

    NASA Technical Reports Server (NTRS)

    Kozlov, I. A.; Nielsen, P. E.; Orgel, L. E.; Bada, J. L. (Principal Investigator)

    1998-01-01

    A novel approach to the radioactive labeling of peptides and PNA oligomers is described. It is based on the conjugation of a deoxynucleoside 3'-phosphate with the terminal amine of the substrate, followed by phosphorylation of the 5'-hydroxyl group of the nucleotide using T4 polynucleotide kinase and [gamma-32P]ATP.

  13. White organic light-emitting diodes with ultra-thin mixed emitting layer

    NASA Astrophysics Data System (ADS)

    Jeon, T.; Forget, S.; Chenais, S.; Geffroy, B.; Tondelier, D.; Bonnassieux, Y.; Ishow, E.

    2012-02-01

    White light can be obtained from Organic Light Emitting Diodes by mixing three primary colors, (i.e. red, green and blue) or two complementary colors in the emissive layer. In order to improve the efficiency and stability of the devices, a host-guest system is generally used as an emitting layer. However, the color balance to obtain white light is difficult to control and optimize because the spectrum is very sensitive to doping concentration (especially when a small amount of material is used). We use here an ultra-thin mixed emitting layer (UML) deposited by thermal evaporation to fabricate white organic light emitting diodes (WOLEDs) without co-evaporation. The UML was inserted in the hole-transporting layer consisting of 4, 4'-bis[N-(1-naphtyl)-N-phenylamino]biphenyl (α-NPB) instead of using a conventional doping process. The UML was formed from a single evaporation boat containing a mixture of two dipolar starbust triarylamine molecules (fvin and fcho) presenting very similar structures and thermal properties and emitting in complementary spectral regions (orange and blue respectively) and mixed according to their weight ratio. The composition of the UML specifically allows for fine tuning of the emission color despite its very thin thickness down to 1 nm. Competitive energy transfer processes from fcho and the host interface toward fvin are key parameters to control the relative intensity between red and blue emission. White light with very good CIE 1931 color coordinate (0.34, 0.34) was obtained by simply adjusting the UML film composition.

  14. Stacking Searches for Greater Than 100 MeV Gamma Ray Emission from Radio Galaxies and Seyfert Galaxies

    NASA Technical Reports Server (NTRS)

    Cillis, A. N.; Hartman, R. C.; Bertsch, D. L.

    2003-01-01

    The EGRET telescope on CGRO detected more than sixty sources of high-energy gamma radiation associated with active galactic nuclei (AGN). All but one of those belong to the blazar subclass; the only exception is the nearby radio galaxy Centaurus A. Since there is no obvious reason other than proximity to expect Cen A to be the only non-blazar AGN emitting in high-energy gamma rays, we have utilized the "stacking" technique to search for $>100$-MeV emission from two non-blazar AGN subclasses, radio galaxies and Seyfert galaxies. Maps of gamma-ray counts, exposure, and diffuse background have been created, then co-added in varying numbers based on sorts by redshift, 5-GHZ flux density, and optical brightness, and finally tested for gamma-ray emission. No detection significance greater than $2\\sigma$ has been found for any subclass, sorting parameter, or number of objects co-added. Monte Carlo simulations have also been performed, to validate the technique and estimate the significance of the results.

  15. Photoactive ligands probing the sweet taste receptor. Design and synthesis of highly potent diazirinyl D-phenylalanine derivatives.

    PubMed

    Masuda, Katsuyoshi; Koizumi, Ayako; Misaka, Takumi; Hatanaka, Yasumaru; Abe, Keiko; Tanaka, Takaharu; Ishiguro, Masaji; Hashimoto, Makoto

    2010-02-01

    Some D-amino acids such as d-tryptophan and D-phenylalanine are well known as naturally-occurring sweeteners. Photoreactive D-phenylalanine derivatives containing trifluoromethyldiazirinyl moiety at 3- or 4-position of phenylalanine, were designed as sweeteners for functional analysis with photoaffinity labeling. The trifluoromethyldiazirinyl D-phenylalanine derivatives were prepared effectively with chemo-enzymatic methods using L-amino acid oxidase and were found to have potent activity toward the human sweet taste receptor. Copyright (c) 2009 Elsevier Ltd. All rights reserved.

  16. Discrimination of irradiated MOX fuel from UOX fuel by multivariate statistical analysis of simulated activities of gamma-emitting isotopes

    NASA Astrophysics Data System (ADS)

    Åberg Lindell, M.; Andersson, P.; Grape, S.; Hellesen, C.; Håkansson, A.; Thulin, M.

    2018-03-01

    This paper investigates how concentrations of certain fission products and their related gamma-ray emissions can be used to discriminate between uranium oxide (UOX) and mixed oxide (MOX) type fuel. Discrimination of irradiated MOX fuel from irradiated UOX fuel is important in nuclear facilities and for transport of nuclear fuel, for purposes of both criticality safety and nuclear safeguards. Although facility operators keep records on the identity and properties of each fuel, tools for nuclear safeguards inspectors that enable independent verification of the fuel are critical in the recovery of continuity of knowledge, should it be lost. A discrimination methodology for classification of UOX and MOX fuel, based on passive gamma-ray spectroscopy data and multivariate analysis methods, is presented. Nuclear fuels and their gamma-ray emissions were simulated in the Monte Carlo code Serpent, and the resulting data was used as input to train seven different multivariate classification techniques. The trained classifiers were subsequently implemented and evaluated with respect to their capabilities to correctly predict the classes of unknown fuel items. The best results concerning successful discrimination of UOX and MOX-fuel were acquired when using non-linear classification techniques, such as the k nearest neighbors method and the Gaussian kernel support vector machine. For fuel with cooling times up to 20 years, when it is considered that gamma-rays from the isotope 134Cs can still be efficiently measured, success rates of 100% were obtained. A sensitivity analysis indicated that these methods were also robust.

  17. ENERGETIC GAMMA RADIATION FROM RAPIDLY ROTATING BLACK HOLES

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hirotani, Kouichi; Pu, Hung-Yi, E-mail: hirotani@tiara.sinica.edu.tw

    2016-02-10

    Supermassive black holes (BHs) are believed to be the central powerhouse of active galactic nuclei. Applying the pulsar outer-magnetospheric particle accelerator theory to BH magnetospheres, we demonstrate that an electric field is exerted along the magnetic field lines near the event horizon of a rotating BH. In this particle accelerator (or a gap), electrons and positrons are created by photon–photon collisions and accelerated in the opposite directions by this electric field, efficiently emitting gamma-rays via curvature and inverse-Compton processes. It is shown that a gap arises around the null-charge surface formed by the frame-dragging effect, provided that there is nomore » current injection across the gap boundaries. The gap is dissipating a part of the hole’s rotational energy, and the resultant gamma-ray luminosity increases with decreasing plasma accretion from the surroundings. Considering an extremely rotating supermassive BH, we show that such a gap reproduces the significant very-high-energy (VHE) gamma-ray flux observed from the radio galaxy IC 310, provided that the accretion rate becomes much less than the Eddington rate particularly during its flare phase. It is found that the curvature process dominates the inverse-Compton process in the magnetosphere of IC 310, and that the observed power-law-like spectrum in VHE gamma-rays can be explained to some extent by a superposition of the curvature emissions with varying curvature radius. It is predicted that the VHE spectrum extends into higher energies with increasing VHE photon flux.« less

  18. Proximity-Induced Covalent Labeling of Proteins with a Reactive Fluorophore-Binding Peptide Tag.

    PubMed

    Sunbul, Murat; Nacheva, Lora; Jäschke, Andres

    2015-08-19

    Labeling of proteins with fluorescent dyes in live cells enables the investigation of their roles in biological systems by fluorescence microscopy. Because the labeling procedure should not disturb the native function of the protein of interest, it is of high importance to find the optimum labeling method for the problem to be studied. Here, we developed a rapid one-step method to covalently and site-specifically label proteins with a TexasRed fluorophore in vitro and in live bacteria. To this end, a genetically encodable TexasRed fluorophore-binding peptide (TR512) was converted into a reactive tag (ReacTR) by adjoining a cysteine residue which rapidly reacts with N-α-chloroacetamide-conjugated TexasRed fluorophore owing to the proximity effect; ReacTR tag first binds to the TexasRed fluorophore and this interaction brings the nucleophilic cysteine and the electrophilic N-α-chloroacetamide groups in close proximity. Our method has several advantages over existing methods: (i) it utilizes a peptide tag much smaller than fluorescent proteins, the SNAP, CLIP, or HaLo tags; (ii) it allows for labeling of proteins with a small, photostable, red-emitting TexasRed fluorophore; (iii) the probe used is very easy to synthesize; (iv) no enzyme is required to transfer the fluorophore to the peptide tag; and (v) labeling yields a stable covalent product in a very fast reaction.

  19. The 4U 0115+63: Another energetic gamma ray binary pulsar

    NASA Technical Reports Server (NTRS)

    Chadwick, P. M.; Dipper, N. A.; Dowthwaite, J. C.; Kirkman, I. W.; Mccomb, T. J. L.; Orford, K. J.; Turver, K. E.

    1985-01-01

    Following the discovery of Her X-1 as a source of pulsed 1000 Gev X-rays, a search for emission from an X-ray binary containing a pulsar with similar values of period, period derivative and luminosity was successful. The sporadic X-ray binary 4U 0115-63 has been observed, with probability 2.5 x 10 to the minus 6 power ergs/s to emit 1000 GeV gamma-rays with a time averaged energy flux of 6 to 10 to the 35th power.

  20. In situ gamma-spectrometry several years after deposition of radiocesium. II. Peak-to-valley method.

    PubMed

    Gering, F; Hillmann, U; Jacob, P; Fehrenbacher, G

    1998-12-01

    A new method is introduced for deriving radiocesium soil contaminations and kerma rates in air from in situ gamma-ray spectrometric measurements. The approach makes use of additional information about gamma-ray attenuation given by the peak-to-valley ratio, which is the ratio of the count rates for primary and forward scattered photons. In situ measurements are evaluated by comparing the experimental data with the results of Monte Carlo simulations of photon transport and detector response. The influence of photons emitted by natural radionuclides on the calculation of the peak-to-valley ratio is carefully analysed. The new method has been applied to several post-Chernobyl measurements and the results agreed well with those of soil sampling.

  1. Did A Galactic Gamma-Ray Burst Kill the Dinosaurs?

    NASA Astrophysics Data System (ADS)

    Brecher, K.

    1997-12-01

    Gamma-ray bursts now appear to be primarily of extragalactic origin. Statistically, assuming isotropic emission, the observed event rates and fluxes imply that one event occurs per 10(4) \\ - 10(6) \\ years per galaxy, with about 10(51) \\ - 10(53) \\ ergs in gamma-rays emitted per event. Unless the Milky Way is unusual, a gamma-ray burst should occur within 10(2) \\ - 10(3) \\ pc of the Sun in a time span of order 10(8) \\ years. Independent of the underlying cause of the event, it would irradiate the solar system with a brief flash of MeV gamma-rays with a fluence as large as 10(9) - 10(11) \\ erg cm(-2) . What is the effect of such an event on the Earth and objects nearby? Ruderman (\\underbar{Science}, 184, 1079, 1974) and subsequent authors have considered a number of effects of a flash of gamma-rays from a nearby supernova explosion on the Earth's atmosphere, and on its biota. However, with regard to the demise of the dinosaurs, it appears that there was a marked increase in the deposition rate of the rare earth iridium coincident with their extinction. For this reason, an asteroid-Earth impact has been considered the leading contender for the death of the dinosaurs. Here we consider a new mechanism for mass biological extinctions, caused by small comets nudged into the inner solar system by nearby gamma-ray bursts. If comets populate the Oort cloud with a wide distribution of masses, radii and orbital eccentricities, we find that small (< 1 km), low density (10(-2) \\ gm cm(-3) ) objects in highly eccentric orbits can be injected into the inner solar system by a nearby gamma-ray burst. For a relatively brief period of time, the near Earth comet population would increase dramatically. The consequent increased probability of comet-Earth impacts of appropriate energy and material content could account for many of the characteristics of the Cretaceous-Tertiary or other terrestrial mass biological extinctions.

  2. The diageotropica mutant of tomato lacks high specific activity auxin binding sites

    NASA Technical Reports Server (NTRS)

    Hicks, G. R.; Rayle, D. L.; Lomax, T. L.

    1989-01-01

    Tomato plants homozygous for the diageotropica (dgt) mutation exhibit morphological and physiological abnormalities which suggest that they are unable to respond to the plant growth hormone auxin (indole-3-acetic acid). The photoaffinity auxin analog [3H]5N3-IAA specifically labels a polypeptide doublet of 40 and 42 kilodaltons in membrane preparations from stems of the parental variety, VFN8, but not from stems of plants containing the dgt mutation. In roots of the mutant plants, however, labeling is indistinguishable from that in VFN8. These data suggest that the two polypeptides are part of a physiologically important auxin receptor system, which is altered in a tissue-specific manner in the mutant.

  3. Observations with the High Altitude GAmma Ray (HAGAR) telescope array in the Indian Himalayas

    NASA Astrophysics Data System (ADS)

    Britto, R. J.; Acharya, B. S.; Anupama, G. C.; Bhatt, N.; Bhattacharjee, P.; Bhattacharya, S. S.; Chitnis, V. R.; Cowsik, R.; Dorji, N.; Duhan, S. K.; Gothe, K. S.; Kamath, P. U.; Koul, R.; Mahesh, P. K.; Mitra, A.; Nagesh, B. K.; Parmar, N. K.; Prabhu, T. P.; Rannot, R. C.; Rao, S. K.; Saha, L.; Saleem, F.; Saxena, A. K.; Sharma, S. K.; Shukla, A.; Singh, B. B.; Srinivasan, R.; Srinivasulu, G.; Sudersanan, P. V.; Tickoo, A. K.; Tsewang, D.; Upadhya, S.; Vishwanath, P. R.; Yadav, K. K.

    2010-12-01

    For several decades, it was thought that astrophysical sources emit high energy photons within the energy range of the gamma-ray region of the electromagnetic spectrum also. These photons originate from interactions of high energy particles from sources involving violent phenomena in the Universe (supernovae, pulsars, Active Galactic Nuclei, etc.) with gas and radiation fields. Since the first reliable detections of cosmic gamma rays in the 1970's, improvements in instrumentation have led gamma-ray astronomy to an established branch of modern Astrophysics, with a constant increase in the number of detected sources. But the 30-300 GeV energy range remained sparsely explored until the launch of the Fermi space telescope in June 2008. The ground-based gamma-ray telescope array HAGAR is the first array of atmospheric Cherenkov telescopes established at a so high altitude (4270 m a.s.l.), and was designed to reach a relatively low energy threshold with quite a low mirror area (31 m^2). It is located at Hanle in India, in the Ladakh region of the Himalayas. Regular source observations have begun with the complete setup of 7 telescopes on Sept. 2008. We report and discuss our estimation of the systematics through dark region studies, and present preliminary results from gamma-ray sources in this paper.

  4. Online gamma-camera imaging of 103Pd seeds (OGIPS) for permanent breast seed implantation

    NASA Astrophysics Data System (ADS)

    Ravi, Ananth; Caldwell, Curtis B.; Keller, Brian M.; Reznik, Alla; Pignol, Jean-Philippe

    2007-09-01

    Permanent brachytherapy seed implantation is being investigated as a mode of accelerated partial breast irradiation for early stage breast cancer patients. Currently, the seeds are poorly visualized during the procedure making it difficult to perform a real-time correction of the implantation if required. The objective was to determine if a customized gamma-camera can accurately localize the seeds during implantation. Monte Carlo simulations of a CZT based gamma-camera were used to assess whether images of suitable quality could be derived by detecting the 21 keV photons emitted from 74 MBq 103Pd brachytherapy seeds. A hexagonal parallel hole collimator with a hole length of 38 mm, hole diameter of 1.2 mm and 0.2 mm septa, was modeled. The design of the gamma-camera was evaluated on a realistic model of the breast and three layers of the seed distribution (55 seeds) based on a pre-implantation CT treatment plan. The Monte Carlo simulations showed that the gamma-camera was able to localize the seeds with a maximum error of 2.0 mm, using only two views and 20 s of imaging. A gamma-camera can potentially be used as an intra-procedural image guidance system for quality assurance for permanent breast seed implantation.

  5. Characterizing near-surface elemental layering on Mars using gamma-ray spectroscopy: A proof-of-principle experiment

    NASA Astrophysics Data System (ADS)

    Peplowski, Patrick N.; Wilson, Jack T.; Beck, Andrew W.; Burks, Morgan; Goldsten, John O.; Lawrence, David J.

    2018-01-01

    Gamma-ray spectroscopy investigations characterize the chemical composition of planetary surfaces by measuring element-characteristic gamma rays with energies of ∼100 keV to ∼9 MeV. Over this energy range, the mean free path of a gamma ray varies from about 1 to 25 cm, therefore gamma-ray measurements sample subsurface composition. Many elements emit gamma rays at multiple, often widely spaced energies, so gamma-ray measurements can in principle also be used to identify depth-dependent variations in subsurface composition. We report results from laboratory measurements and radiation transport modeling designed to demonstrate this capability. The laboratory measurements verified the presence of depth-dependent gamma-ray signatures, and were then used to benchmark radiation transport simulations that were used to model realistic Mars-like scenarios. The models indicate that compositionally distinct subsurface deposits, buried to depths of ∼80 cm (125 g/cm2), can be identified using gamma-ray measurements. Going beyond identification to characterization (burial depth, relative composition of the layers) of the deposits requires knowledge of the vertical and horizontal variability in the water content of the near-surface surface materials, the local Galactic Cosmic Ray environment (magnitude and energy distribution), the depth-dependent neutron flux, gamma-ray production cross sections, and knowledge of the composition and column density of the atmosphere. The results of our experiments and models provided a basis for examining the utility of using orbiter- and lander-based gamma-ray measurements to identify subsurface deposits on Mars.

  6. Search for gamma-ray emission from Galactic novae with the Fermi -LAT

    NASA Astrophysics Data System (ADS)

    Franckowiak, A.; Jean, P.; Wood, M.; Cheung, C. C.; Buson, S.

    2018-02-01

    Context. A number of novae have been found to emit high-energy gamma rays (>100 MeV). However, the origin of this emission is not yet understood. We report on the search for gamma-ray emission from 75 optically detected Galactic novae in the first 7.4 years of operation of the Fermi Large Area Telescope using the Pass 8 data set. Aims: We compile an optical nova catalog including light curves from various resources and estimate the optical peak time and optical peak magnitude in order to search for gamma-ray emission to determine whether all novae are gamma-ray emitters. Methods: We repeated the analysis of the six novae previously identified as gamma-ray sources and developed a unified analysis strategy that we then applied to all novae in our catalog. We searched for emission in a 15 day time window in two-day steps ranging from 20 days before to 20 days after the optical peak time. We performed a population study with Monte Carlo simulations to set constraints on the properties of the gamma-ray emission of novae. Results: Two new novae candidates have been found at 2σ global significance. Although these two novae candidates were not detected at a significant level individually, taking them together with the other non-detected novae, we found a sub-threshold nova population with a cumulative 3σ significance. We report the measured gamma-ray flux for detected sources and flux upper limits for novae without significant detection. Our results can be reproduced by several gamma-ray emissivity models (e.g., a power-law distribution with a slope of 2), while a constant emissivity model (i.e., assuming novae are standard candles) can be rejected.

  7. Search for gamma-ray emission from Galactic novae with the Fermi-LAT

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Franckowiak, A.; Jean, P.; Wood, M.

    Context. A number of novae have been found to emit high-energy gamma rays (>100 MeV). However, the origin of this emission is not yet understood. We report on the search for gamma-ray emission from 75 optically detected Galactic novae in the first 7.4 years of operation of the Fermi Large Area Telescope using the Pass 8 data set. Aims. We compile an optical nova catalog including light curves from various resources and estimate the optical peak time and optical peak magnitude in order to search for gamma-ray emission to determine whether all novae are gamma-ray emitters. Methods. We repeated themore » analysis of the six novae previously identified as gamma-ray sources and developed a unified analysis strategy that we then applied to all novae in our catalog. We searched for emission in a 15 day time window in two-day steps ranging from 20 days before to 20 days after the optical peak time. We performed a population study with Monte Carlo simulations to set constraints on the properties of the gamma-ray emission of novae. Results. Two new novae candidates have been found at ~ 2σ global significance. Although these two novae candidates were not detected at a significant level individually, taking them together with the other non-detected novae, we found a sub-threshold nova population with a cumulative 3σ significance. We report the measured gamma-ray flux for detected sources and flux upper limits for novae without significant detection. Lastly, our results can be reproduced by several gamma-ray emissivity models (e.g., a power-law distribution with a slope of 2), while a constant emissivity model (i.e., assuming novae are standard candles) can be rejected.« less

  8. Search for gamma-ray emission from Galactic novae with the Fermi-LAT

    DOE PAGES

    Franckowiak, A.; Jean, P.; Wood, M.; ...

    2018-02-05

    Context. A number of novae have been found to emit high-energy gamma rays (>100 MeV). However, the origin of this emission is not yet understood. We report on the search for gamma-ray emission from 75 optically detected Galactic novae in the first 7.4 years of operation of the Fermi Large Area Telescope using the Pass 8 data set. Aims. We compile an optical nova catalog including light curves from various resources and estimate the optical peak time and optical peak magnitude in order to search for gamma-ray emission to determine whether all novae are gamma-ray emitters. Methods. We repeated themore » analysis of the six novae previously identified as gamma-ray sources and developed a unified analysis strategy that we then applied to all novae in our catalog. We searched for emission in a 15 day time window in two-day steps ranging from 20 days before to 20 days after the optical peak time. We performed a population study with Monte Carlo simulations to set constraints on the properties of the gamma-ray emission of novae. Results. Two new novae candidates have been found at ~ 2σ global significance. Although these two novae candidates were not detected at a significant level individually, taking them together with the other non-detected novae, we found a sub-threshold nova population with a cumulative 3σ significance. We report the measured gamma-ray flux for detected sources and flux upper limits for novae without significant detection. Lastly, our results can be reproduced by several gamma-ray emissivity models (e.g., a power-law distribution with a slope of 2), while a constant emissivity model (i.e., assuming novae are standard candles) can be rejected.« less

  9. In-vivo Diagnosis of Breast Cancer Using Gamma Stimulated Emission Computed Tomography

    DTIC Science & Technology

    2011-04-01

    2006. [9] Floyd CE, Howell CR, Harrawood BP, Crowell AS, Kapadia AJ, Macri R, Xia JQ, Pedroni R, Bowsher J, Kiser MR, Tourassi GD, Tornow W , and...spin-sequence 0-1-2), with emitted gamma-ray energy 3448keV, 2601keV, 2657.562keV. In our simulation, w take tw m jor de-excitation parts into... Walter R, "Neutron Stimulated Emission Computed Tomography of Stable Isotopes," Proceedings of SPIE Medical Imaging 2004, vol. 5368, pp. 248-254. 17

  10. Accurate Modeling of the Terrestrial Gamma-Ray Background for Homeland Security Applications

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sandness, Gerald A.; Schweppe, John E.; Hensley, Walter K.

    2009-10-24

    Abstract–The Pacific Northwest National Laboratory has developed computer models to simulate the use of radiation portal monitors to screen vehicles and cargo for the presence of illicit radioactive material. The gamma radiation emitted by the vehicles or cargo containers must often be measured in the presence of a relatively large gamma-ray background mainly due to the presence of potassium, uranium, and thorium (and progeny isotopes) in the soil and surrounding building materials. This large background is often a significant limit to the detection sensitivity for items of interest and must be modeled accurately for analyzing homeland security situations. Calculations ofmore » the expected gamma-ray emission from a disk of soil and asphalt were made using the Monte Carlo transport code MCNP and were compared to measurements made at a seaport with a high-purity germanium detector. Analysis revealed that the energy spectrum of the measured background could not be reproduced unless the model included gamma rays coming from the ground out to distances of at least 300 m. The contribution from beyond about 50 m was primarily due to gamma rays that scattered in the air before entering the detectors rather than passing directly from the ground to the detectors. These skyshine gamma rays contribute tens of percent to the total gamma-ray spectrum, primarily at energies below a few hundred keV. The techniques that were developed to efficiently calculate the contributions from a large soil disk and a large air volume in a Monte Carlo simulation are described and the implications of skyshine in portal monitoring applications are discussed.« less

  11. Lightning leader models of terrestrial gamma-ray flashes

    NASA Astrophysics Data System (ADS)

    Dwyer, J. R.; Liu, N.; Ihaddadene, K. M. A.

    2017-12-01

    Terrestrial gamma-ray flashes (TGFs) are bright sub-millisecond bursts of gamma rays that originate from thunderstorms. Because lightning leaders near the ground have been observed to emit x-rays, presumably due to runaway electron production in the high-field regions near the leader tips, models of TGFs have been developed by several groups that assume a similar production mechanism of runaway electrons from lightning leaders propagating through thunderclouds. However, it remains unclear exactly how and where these runaway electrons are produced, since lightning propagation at thunderstorm altitudes remains poorly understood. In addition, it is not obvious how to connect the observed behavior of the x-ray production from lightning near the ground with the properties of TGFs. For example, it is not clear how to relate the time structure of the x-ray emission near the ground to that of TGFs, since x-rays from stepped leaders near the ground are usually produced in a series of sub-microsecond bursts, but TGFs are usually observed as much longer pulses without clear substructures, at sub-microsecond timescales or otherwise. In this presentation, spacecraft observations of TGFs, ground-based observations of x-rays from lightning and laboratory sparks, and Monte Carlo and PIC simulations of runaway electron and gamma ray production and propagation will be used to constrain the lightning leader models of TGFs.

  12. The Imprint of the Extragalactic Background Light in the Gamma-Ray Spectra of Blazars

    DOE PAGES

    Ackermann, M.; Ajello, M.; Allafort, A.; ...

    2012-11-30

    The light emitted by stars and accreting compact objects through the history of the universe is encoded in the intensity of the extragalactic background light (EBL). Knowledge of the EBL is important to understand the nature of star formation and galaxy evolution, but direct measurements of the EBL are limited by galactic and other foreground emissions. In this paper, we report an absorption feature seen in the combined spectra of a sample of gamma-ray blazars out to a redshift of z ~ 1.6. Finally, this feature is caused by attenuation of gamma rays by the EBL at optical to ultravioletmore » frequencies and allowed us to measure the EBL flux density in this frequency band.« less

  13. Characteristics of blue organic light emitting diodes with different thick emitting layers

    NASA Astrophysics Data System (ADS)

    Li, Chong; Tsuboi, Taiju; Huang, Wei

    2014-08-01

    We fabricated blue organic light emitting diodes (called blue OLEDs) with emitting layer (EML) of diphenylanthracene derivative 9,10-di(2-naphthyl)anthracene (ADN) doped with blue-emitting DSA-ph (1-4-di-[4-(N,N-di-phenyl)amino]styryl-benzene) to investigate how the thickness of EML and hole injection layer (HIL) influences the electroluminescence characteristics. The driving voltage was observed to increase with increasing EML thickness from 15 nm to 70 nm. The maximum external quantum efficiency of 6.2% and the maximum current efficiency of 14 cd/A were obtained from the OLED with 35 nm thick EML and 75 nm thick HIL. High luminance of 120,000 cd/m2 was obtained at 7.5 V from OLED with 15 nm thick EML.

  14. Tissue-equivalent TL sheet dosimetry system for X- and gamma-ray dose mapping.

    PubMed

    Nariyama, N; Konnai, A; Ohnishi, S; Odano, N; Yamaji, A; Ozasa, N; Ishikawa, Y

    2006-01-01

    To measure dose distribution for X- and gamma rays simply and accurately, a tissue-equivalent thermoluminescent (TL) sheet-type dosemeter and reader system were developed. The TL sheet is composed of LiF:Mg,Cu,P and ETFE polymer, and the thickness is 0.2 mm. For the TL reading, a square heating plate, 20 cm on each side, was developed, and the temperature distribution was measured with an infrared thermal imaging camera. As a result, linearity within 2% and the homogeneity within 3% were confirmed. The TL signal emitted is detected using a CCD camera and displayed as a spatial dose distribution. Irradiation using synchrotron radiation between 10 and 100 keV and (60)Co gamma rays showed that the TL sheet dosimetry system was promising for radiation dose mapping for various purposes.

  15. Characterisation of imperial college reactor centre legacy waste using gamma-ray spectrometry

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shuhaimi, Alif Imran Mohd

    Waste characterisation is a principal component in waste management strategy. The characterisation includes identification of chemical, physical and radiochemical parameters of radioactive waste. Failure to determine specific waste properties may result in sentencing waste packages which are not compliant with the regulation of long term storage or disposal. This project involved measurement of intensity and energy of gamma photons which may be emitted by radioactive waste generated during decommissioning of Imperial College Reactor Centre (ICRC). The measurement will use High Purity Germanium (HPGe) as Gamma-ray detector and ISOTOPIC-32 V4.1 as analyser. In order to ensure the measurements provide reliable results,more » two quality control (QC) measurements using difference matrices have been conducted. The results from QC measurements were used to determine the accuracy of the ISOTOPIC software.« less

  16. Addressing the third gamma problem in PET

    NASA Astrophysics Data System (ADS)

    Schueller, M. J.; Mulnix, T. L.; Christian, B. T.; Jensen, M.; Holm, S.; Oakes, T. R.; Roberts, A. D.; Dick, D. W.; Martin, C. C.; Nickles, R. J.

    2003-02-01

    PET brings the promise of quantitative imaging of the in-vivo distribution of any positron emitting nuclide, a list with hundreds of candidates. All but a few of these, the "pure positron" emitters, have isotropic coincident gamma rays that give rise to misrepresented events in the sinogram and in the resulting reconstructed image. Of particular interest are /sup 10/C, /sup 14/O, /sup 38/K, /sup 52m/Mn, /sup 60/Cu, /sup 61/Cu, /sup 94m/Tc, and /sup 124/I, each having high-energy gammas that are Compton-scattered down into the 511 keV window. The problems arising from the "third gamma," and its accommodation by standard scatter correction algorithms, were studied empirically, employing three scanner models (CTI 933/04, CTI HR+ and GE Advance), imaging three phantoms (line source, NEMA scatter and contrast/detail), with /sup 18/F or /sup 38/K and /sup 72/As mimicking /sup 14/O and /sup 10/C, respectively, in 2-D and 3-D modes. Five findings emerge directly from the image analysis. The third gamma: 1) does, obviously, tax the single event rate of the PET scanners, particularly in the absence of septa, from activity outside of the axial field of view; 2) does, therefore, tax the random rate, which is second order in singles, although the gamma is a prompt coincidence partner; 3) does enter the sinogram as an additional flat background, like randoms, but unlike scatter; 4) is not seriously misrepresented by the scatter algorithm which fits the correction to the wings of the sinogram; and 5) does introduce additional statistical noise from the subsequent subtraction, but does not seriously compromise the detectability of lesions as seen in the contrast/detail phantom. As a safeguard against the loss of accuracy in image quantitation, fiducial sources of known activity are included in the field of view alongside of the subject. With this precaution, a much wider selection of imaging agents can enjoy the advantages of positron emission tomography.

  17. Mapping General Anesthetic Sites in Heteromeric γ-Aminobutyric Acid Type A Receptors Reveals a Potential For Targeting Receptor Subtypes.

    PubMed

    Forman, Stuart A; Miller, Keith W

    2016-11-01

    IV general anesthetics, including propofol, etomidate, alphaxalone, and barbiturates, produce important actions by enhancing γ-aminobutyric acid type A (GABAA) receptor activation. In this article, we review scientific studies that have located and mapped IV anesthetic sites using photoaffinity labeling and substituted cysteine modification protection. These anesthetics bind in transmembrane pockets between subunits of typical synaptic GABAA receptors, and drugs that display stereoselectivity also show remarkably selective interactions with distinct interfacial sites. These results suggest strategies for developing new drugs that selectively modulate distinct GABAA receptor subtypes.

  18. Lunar elemental analysis obtained from the Apollo gamma-ray and X-ray remote sensing experiment

    NASA Technical Reports Server (NTRS)

    Trombka, J. I.; Arnold, J. R.; Adler, I.; Metzger, A. E.; Reedy, R. C.

    1974-01-01

    Gamma ray and X-ray spectrometers carried in the service module of the Apollo 15 and 16 spacecraft were employed for compositional mapping of the lunar surface. The measurements involved the observation of the intensity and characteristics energy distribution of gamma rays and X-rays emitted from the lunar surface. A large scale compositional map of over 10 percent of the lunar surface was obtained from an analysis of the observed spectra. The objective of the X-ray experiment was to measure the K spectral lines from Mg, Al, and Si. Spectra were obtained and the data were reduced to Al/Si and Mg/Si intensity ratios and ultimately to chemical ratios. The objective of the gamma-ray experiment was to measure the natural and cosmic ray induced activity emission spectrum. At this time, the elemental abundances for Th, U, K, Fe, Ti, Si, and O have been determined over a number of major lunar regions.

  19. Fermi-LAT Detection of Gravitational Lens Delayed Gamma-Ray Flares from Blazar B0218+357

    NASA Technical Reports Server (NTRS)

    Cheung, C. C.; Larsson, S.; Scargle, J. D.; Amin, M. A.; Blandford, R. D.; Bulmash, D.; Chiang, J.; Ciprini, S.; Corbet, R. D. H.; Falco, E. E.; hide

    2014-01-01

    Using data from the Fermi Large Area Telescope (LAT), we report the first clear gamma-ray measurement of a delay between flares from the gravitationally lensed images of a blazar. The delay was detected in B0218+357, a known double-image lensed system, during a period of enhanced gamma-ray activity with peak fluxes consistently observed to reach greater than 20-50 times its previous average flux. An auto-correlation function analysis identified a delay in the gamma-ray data of 11.46 plus or minus 0.16 days (1 sigma) that is approximately 1 day greater than previous radio measurements. Considering that it is beyond the capabilities of the LAT to spatially resolve the two images, we nevertheless decomposed individual sequences of superposing gamma-ray flares/delayed emissions. In three such approximately 8-10 day-long sequences within an approximately 4-month span, considering confusion due to overlapping flaring emission and flux measurement uncertainties, we found flux ratios consistent with approximately 1, thus systematically smaller than those from radio observations. During the first, best-defined flare, the delayed emission was detailed with a Fermi pointing, and we observed flux doubling timescales of approximately 3-6 hours implying as well extremely compact gamma-ray emitting regions.

  20. Molecular radiotheragnostics in prostate cancer.

    PubMed

    Du, Yong; Dizdarevic, Sabina

    2017-10-01

    Two different molecular radio-theragnostic principles are applied in prostate cancer, providing a personalised management for those patients. Firstly, radiopharmaceuticals with the same or similar mechanism of action but different energy (gamma-γ, eg 99m Tc-diphosphonates or positron-β+, eg 18F-NaF emitting isotopes) can be used to identify patients with osteoblastic metastases for a treatment with bone seeking beta (β-) or alpha (α-) emitting radionuclides to deliver targeted molecular radiotherapy. A number of such β- emitting molecules have been used for bone palliation. More recently, an alpha emitting 223 Ra-dicholoride demonstrated not only symptomatic relief but also significantly improved overall survival in castration-resistant prostate cancer with predominant bone metastases. The second principle involves utilisation of the same prostatic specific membrane antigen (PSMA) or similar compound (eg PSMA-11, PSMA-617), but different label with either β+ ( 68 Ga) or γ ( 99m Tc) emitting radioisotope for imaging and subsequently β- ( 177 Lu) or α ( 225 Ac) emitting radionuclide for treatment. © Royal College of Physicians 2017. All rights reserved.

  1. Dual-isotope PET using positron-gamma emitters.

    PubMed

    Andreyev, A; Celler, A

    2011-07-21

    Positron emission tomography (PET) is widely recognized as a highly effective functional imaging modality. Unfortunately, standard PET cannot be used for dual-isotope imaging (which would allow for simultaneous investigation of two different biological processes), because positron-electron annihilation products from different tracers are indistinguishable in terms of energy. Methods that have been proposed for dual-isotope PET rely on differences in half-lives of the participating isotopes; these approaches, however, require making assumptions concerning kinetic behavior of the tracers and may not lead to optimal results. In this paper we propose a novel approach for dual-isotope PET and investigate its performance using GATE simulations. Our method requires one of the two radioactive isotopes to be a pure positron emitter and the second isotope to emit an additional high-energy gamma in a cascade simultaneously with positron emission. Detection of this auxiliary prompt gamma in coincidence with the annihilation event allows us to identify the corresponding 511 keV photon pair as originating from the same isotope. Two list-mode datasets are created: a primary dataset that contains all detected 511 keV photon pairs from both isotopes, and a second, tagged (much smaller) dataset that contains only those PET events for which a coincident prompt gamma has also been detected. An image reconstructed from the tagged dataset reflects the distribution of the second positron-gamma radiotracer and serves as a prior for the reconstruction of the primary dataset. Our preliminary simulation study with partially overlapping (18)F/(22)Na and (18)F/(60)Cu radiotracer distributions showed that in these two cases the dual-isotope PET method allowed for separation of the two activity distributions and recovered total activities with relative errors of about 5%.

  2. Analysis of Nuclear Lifetimes Using the Gamma-ray Induced Doppler Shift Attenuation Method

    NASA Astrophysics Data System (ADS)

    Crespi, F. C. L.

    2018-05-01

    Lifetime measurements allow extraction of fundamental information on the nature of the excited states of a nuclear system. Since nuclear lifetimes cover many orders of magnitude, a number of experimental techniques and detection setups have been developed depending on the range of the lifetime of interest. The Gamma-ray Induced Doppler Shift Attenuation (GRIDSA) Method presented here is applied to the measurement of very short lifetimes, in the femtosecond range. It allows determining the nuclear lifetime by measuring the Doppler shift of a gamma ray emitted from the state of interest, in different directions with respect to a coincident preceding gamma ray, populating the same state and inducing a recoil of the nucleus in the target material with velocities of the order of 104-105 m/s. We realized an experiment in order to test the GRIDSA technique for the measurement of fs lifetimes after (n,γ) reactions. The measurement was performed at the Institut Laue-Langevin (ILL) with the 8 Ge-clover detectors of the FIPPS array. Preliminary results are discussed.

  3. A Detailed Observational Analysis of V1324 Sco, the Most Gamma-Ray-luminous Classical Nova to Date

    NASA Astrophysics Data System (ADS)

    Finzell, Thomas; Chomiuk, Laura; Metzger, Brian D.; Walter, Frederick M.; Linford, Justin D.; Mukai, Koji; Nelson, Thomas; Weston, Jennifer H. S.; Zheng, Yong; Sokoloski, Jennifer L.; Mioduszewski, Amy; Rupen, Michael P.; Dong, Subo; Starrfield, Sumner; Cheung, C. C.; Woodward, Charles E.; Taylor, Gregory B.; Bohlsen, Terry; Buil, Christian; Prieto, Jose; Wagner, R. Mark; Bensby, Thomas; Bond, I. A.; Sumi, T.; Bennett, D. P.; Abe, F.; Koshimoto, N.; Suzuki, D.; Tristram, P. J.; Christie, Grant W.; Natusch, Tim; McCormick, Jennie; Yee, Jennifer; Gould, Andy

    2018-01-01

    It has recently been discovered that some, if not all, classical novae emit GeV gamma-rays during outburst, but the mechanisms involved in the production ofgamma-rays are still not well understood. We present here a comprehensive multiwavelength data set—from radio to X-rays—for the most gamma-ray-luminous classical nova to date, V1324 Sco. Using this data set, we show that V1324 Sco is a canonical dusty Fe II-type nova, with a maximum ejecta velocity of 2600 km s‑1 and an ejecta mass of a few × {10}-5 {M}ȯ . There is also evidence for complex shock interactions, including a double-peaked radio light curve which shows high brightness temperatures at early times. To explore why V1324 Sco was so gamma-ray luminous, we present a model of the nova ejecta featuring strong internal shocks and find that higher gamma-ray luminosities result from higher ejecta velocities and/or mass-loss rates. Comparison of V1324 Sco with other gamma-ray-detected novae does not show clear signatures of either, and we conclude that a larger sample of similarly well-observed novae is needed to understand the origin and variation of gamma-rays in novae.

  4. Prompt gamma-ray imaging for small animals

    NASA Astrophysics Data System (ADS)

    Xu, Libai

    Small animal imaging is recognized as a powerful discovery tool for small animal modeling of human diseases, which is providing an important clue to complete understanding of disease mechanisms and is helping researchers develop and test new treatments. The current small animal imaging techniques include positron emission tomography (PET), single photon emission tomography (SPECT), computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound (US). A new imaging modality called prompt gamma-ray imaging (PGI) has been identified and investigated primarily by Monte Carlo simulation. Currently it is suggested for use on small animals. This new technique could greatly enhance and extend the present capabilities of PET and SPECT imaging from ingested radioisotopes to the imaging of selected non-radioactive elements, such as Gd, Cd, Hg, and B, and has the great potential to be used in Neutron Cancer Therapy to monitor neutron distribution and neutron-capture agent distribution. This approach consists of irradiating small animals in the thermal neutron beam of a nuclear reactor to produce prompt gamma rays from the elements in the sample by the radiative capture (n, gamma) reaction. These prompt gamma rays are emitted in energies that are characteristic of each element and they are also produced in characteristic coincident chains. After measuring these prompt gamma rays by surrounding spectrometry array, the distribution of each element of interest in the sample is reconstructed from the mapping of each detected signature gamma ray by either electronic collimations or mechanical collimations. In addition, the transmitted neutrons from the beam can be simultaneously used for very sensitive anatomical imaging, which provides the registration for the elemental distributions obtained from PGI. The primary approach is to use Monte Carlo simulation methods either with the specific purpose code CEARCPG, developed at NC State University or with the general purpose

  5. HESS J1844-030: A New Gamma-Ray Binary?

    NASA Astrophysics Data System (ADS)

    McCall, Hannah; Errando, Manel

    2018-01-01

    Gamma-ray binaries are comprised of a massive, main-sequence star orbiting a neutron star or black hole that generates bright gamma-ray emission. Only six of these systems have been discovered. Here we report on a candidate stellar-binary system associated with the unidentified gamma-ray source HESS J1844-030, whose detection was revealed in the H.E.S.S. galactic plane survey. Analysis of 60 ks of archival Chandra data and over 100 ks of XMM-Newton data reveal a spatially associated X-ray counterpart to this TeV-emitting source (E>1012 eV), CXO J1845-031. The X-ray spectra derived from these exposures yields column density absorption in the range nH = (0.4 - 0.7) x 1022 cm-2, which is below the total galactic value for that part of the sky, indicating that the source is galactic. The flux from CXO J1845-031 increases with a factor of up to 2.5 in a 60 day timescale, providing solid evidence for flux variability at a confidence level exceeding 7 standard deviations. The point-like nature of the source, the flux variability of the nearby X-ray counterpart, and the low column density absorption are all indicative of a binary system. Once confirmed, HESS J1844-030 would represent only the seventh known gamma-ray binary, providing valuable data to advance our understanding of the physics of pulsars and stellar winds and testing high-energy astrophysical processes at timescales not present in other classes of objects.

  6. Precision imaging of 4.4 MeV gamma rays using a 3-D position sensitive Compton camera.

    PubMed

    Koide, Ayako; Kataoka, Jun; Masuda, Takamitsu; Mochizuki, Saku; Taya, Takanori; Sueoka, Koki; Tagawa, Leo; Fujieda, Kazuya; Maruhashi, Takuya; Kurihara, Takuya; Inaniwa, Taku

    2018-05-25

    Imaging of nuclear gamma-ray lines in the 1-10 MeV range is far from being established in both medical and physical applications. In proton therapy, 4.4 MeV gamma rays are emitted from the excited nucleus of either 12 C* or 11 B* and are considered good indicators of dose delivery and/or range verification. Further, in gamma-ray astronomy, 4.4 MeV gamma rays are produced by cosmic ray interactions in the interstellar medium, and can thus be used to probe nucleothynthesis in the universe. In this paper, we present a high-precision image of 4.4 MeV gamma rays taken by newly developed 3-D position sensitive Compton camera (3D-PSCC). To mimic the situation in proton therapy, we first irradiated water, PMMA and Ca(OH)2 with a 70 MeV proton beam, then we identified various nuclear lines with the HPGe detector. The 4.4 MeV gamma rays constitute a broad peak, including single and double escape peaks. Thus, by setting an energy window of 3D-PSCC from 3 to 5 MeV, we show that a gamma ray image sharply concentrates near the Bragg peak, as expected from the minimum energy threshold and sharp peak profile in the cross section of 12 C(p,p) 12 C*.

  7. Anticonvulsant properties of alpha, gamma, and alpha, gamma-substituted gamma-butyrolactones.

    PubMed

    Klunk, W E; Covey, D F; Ferrendelli, J A

    1982-09-01

    Derivatives of gamma-butyrolactone (GBL) substituted on the alpha- and/or gamma-positions were synthesized and tested for their effects on behavior in mice, on the electroencephalographs and blood pressure of paralyzed-ventilated guinea pigs, and on electrical activity of incubated hippocampal slices. Several compounds, including alpha-ethyl-alpha-methyl GBL (alpha-EMGBL), alpha, alpha-dimethyl GBL, alpha, gamma-diethyl-alpha, gamma-dimethyl GBL, and gamma-ethyl-gamma-methyl GBL, prevented seizures induced by pentylenetetrazol, beta-ethyl-beta-methyl-gamma-butyrolactone (beta-EMGBL), picrotoxin, or all three compounds in mice and guinea pigs but had no effect on seizures induced by maximal electroshock or bicuculline. Neither gamma-hydroxybutyrate (GHB) nor alpha-isopropylidine GBL had any anticonvulsant activity. The anticonvulsant alpha-substituted compounds had a potent hypotensive effect and antagonized the hypertensive effect of beta-EMGBL, alpha-EMGBL was tested in incubated hippocampal slices and was found to depress basal activity and antagonize excitation induced by beta-EMGBL. These results demonstrate that alpha-alkyl-substituted GBL and, to a lesser extent, gamma-substituted derivatives are anticonvulsant agents and that their effects are strikingly different from those of GHB or beta-alkyl-substituted GBLs, which are epileptogenic. Possibly beta- and alpha-substituted GBLs act at the same site as agonists and antagonists, respectively.

  8. Assessing cancer risk in China from γ-hexachlorocyclohexane emitted from Chinese and Indian sources.

    PubMed

    Xu, Yue; Tian, Chongguo; Ma, Jianmin; Wang, Xiaoping; Li, Jun; Tang, Jianhui; Chen, Yingjun; Qin, Wei; Zhang, Gan

    2013-07-02

    Three models, including an atmospheric transport model, a multimedia exposure model, and a risk assessment model, were used to assess cancer risk in China caused by γ-HCH (gamma-hexachlorocyclohexane) emitted from Chinese and Indian sources. Extensive model investigations revealed the contribution of different sources to the cancer risk in China. Cancer risk in Eastern China was primarily attributable to γ-HCH contamination from Chinese sources, whereas cancer risk in Western China was caused mostly by Indian emissions. The contribution of fresh use of lindane in India to the cancer risk in China was almost 1 order of magnitude higher than that of the reemission of γ-HCH from Indian soils. Of total population, 58% (about 0.79 billion) residents in China were found to live in the environment with high levels of cancer risk exceeding the acceptable cancer risk of 10(-6), recommended by the United States Environmental Protection Agency (U.S. EPA). The cancer risk in China was mostly induced by the local contamination of γ-HCH emitted from Chinese sources, whereas fresh use of lindane in India will become a significant source of the cancer risk in China if Indian emissions maintain their current levels.

  9. Low-dose radiation modifies skin response to acute gamma-rays and protons.

    PubMed

    Mao, Xiao Wen; Pecaut, Michael J; Cao, Jeffrey D; Moldovan, Maria; Gridley, Daila S

    2013-01-01

    The goal of the present study was to obtain pilot data on the effects of protracted low-dose/low-dose-rate (LDR) γ-rays on the skin, both with and without acute gamma or proton irradiation (IR). Six groups of C57BL/6 mice were examined: a) 0 Gy control, b) LDR, c) Gamma, d) LDR+Gamma, e) Proton, and f) LDR+Proton. LDR radiation was delivered to a total dose of 0.01 Gy (0.03 cGy/h), whereas the Gamma and Proton groups received 2 Gy (0.9 Gy/min and 1.0 Gy/min, respectively). Assays were performed 56 days after exposure. Skin samples from all irradiated groups had activated caspase-3, indicative of apoptosis. The significant (p<0.05) increases in immunoreactivity in the Gamma and Proton groups were not present when LDR pre-exposure was included. However, the terminal deoxynucleotidyl transferase dUTP nick-end labeling assay for DNA fragmentation and histological examination of hematoxylin and eosin-stained sections revealed no significant differences among groups, regardless of radiation regimen. The data demonstrate that caspase-3 activation initially triggered by both forms of acute radiation was greatly elevated in the skin nearly two months after whole-body exposure. In addition, LDR γ-ray priming ameliorated this response.

  10. Advances in Gamma-Ray Imaging with Intensified Quantum-Imaging Detectors

    NASA Astrophysics Data System (ADS)

    Han, Ling

    Nuclear medicine, an important branch of modern medical imaging, is an essential tool for both diagnosis and treatment of disease. As the fundamental element of nuclear medicine imaging, the gamma camera is able to detect gamma-ray photons emitted by radiotracers injected into a patient and form an image of the radiotracer distribution, reflecting biological functions of organs or tissues. Recently, an intensified CCD/CMOS-based quantum detector, called iQID, was developed in the Center for Gamma-Ray Imaging. Originally designed as a novel type of gamma camera, iQID demonstrated ultra-high spatial resolution (< 100 micron) and many other advantages over traditional gamma cameras. This work focuses on advancing this conceptually-proven gamma-ray imaging technology to make it ready for both preclinical and clinical applications. To start with, a Monte Carlo simulation of the key light-intensification device, i.e. the image intensifier, was developed, which revealed the dominating factor(s) that limit energy resolution performance of the iQID cameras. For preclinical imaging applications, a previously-developed iQID-based single-photon-emission computed-tomography (SPECT) system, called FastSPECT III, was fully advanced in terms of data acquisition software, system sensitivity and effective FOV by developing and adopting a new photon-counting algorithm, thicker columnar scintillation detectors, and system calibration method. Originally designed for mouse brain imaging, the system is now able to provide full-body mouse imaging with sub-350-micron spatial resolution. To further advance the iQID technology to include clinical imaging applications, a novel large-area iQID gamma camera, called LA-iQID, was developed from concept to prototype. Sub-mm system resolution in an effective FOV of 188 mm x 188 mm has been achieved. The camera architecture, system components, design and integration, data acquisition, camera calibration, and performance evaluation are presented in

  11. First NICER Observations of the Enigmatic Be Star, Gamma Cassiopeiae

    NASA Astrophysics Data System (ADS)

    Hamaguchi, Kenji; Drake, Stephen; Corcoran, Michael; Gendreau, Keith C.; Steiner, Jack; NICER team

    2018-01-01

    Gamma Cassiopeiae is an enigmatic Be star with unusually hard, strong X-ray emission compared with normal main-sequence B stars (but a much lower X-ray luminosity than the classic X-ray binaries). The X-ray characteristics - hot (kT ~12 keV), thermal plasma spectrum and rapid time variations on timescales of 10-1000 sec - is reminiscent of accreting compact objects. Alternatively, its shot-like rapid variations somewhat resemble solar-type magnetic reconnection flares, so that plasma heating by the star-disk magnetic dynamo of the Be star has been proposed. A recent discovery of rapid X-ray color variations with the Suzaku X-ray observatory revealed the presence of rapidly moving absorbers in the X-ray line of sight, giving a strong constraint on the geometry of the X-ray emitting regions and absorbers.The X-ray observatory onboard ISS, NICER, has observed Gamma Cas multiple times. The large collecting area in the 0.3-10 keV band, tolerance to photon pile-ups, and decent energy resolution in particular below 1 keV, should provide excellent pieces of information on how the X-ray emission and/or absorbing column change on short timescales. We will present the first result of the gamma Cas observations made by the NICER observatory.

  12. PET-radioimmunodetection of integrins: imaging acute colitis using a ⁶⁴Cu-labeled anti-β₇ integrin antibody.

    PubMed

    Dearling, Jason L J; Packard, Alan B

    2012-01-01

    Integrins are involved in a wide range of cell interactions. Imaging their distribution using high-resolution noninvasive techniques that are directly translatable to the clinic can provide new insights into disease processes and presents the opportunity to directly monitor new therapies. In this chapter, we describe a protocol to image, the in vivo distribution of the integrin β(7), expressed by lymphocytes recruited to and retained by the inflamed gut, using a radiolabeled whole antibody. The antibody is purified, conjugated with a bifunctional chelator for labeling with a radiometal, labeled with the positron-emitting radionuclide (64)Cu, and injected into mice for microPET studies. Mice with DSS-induced colitis were found to have higher uptake of the (64)Cu-labeled antibody in the gut than control groups.

  13. Light emitting ceramic device

    DOEpatents

    Valentine, Paul; Edwards, Doreen D.; Walker, Jr., William John; Slack, Lyle H.; Brown, Wayne Douglas; Osborne, Cathy; Norton, Michael; Begley, Richard

    2010-05-18

    A light-emitting ceramic based panel, hereafter termed "electroceramescent" panel, is herein claimed. The electroceramescent panel is formed on a substrate providing mechanical support as well as serving as the base electrode for the device. One or more semiconductive ceramic layers directly overlay the substrate, and electrical conductivity and ionic diffusion are controlled. Light emitting regions overlay the semiconductive ceramic layers, and said regions consist sequentially of a layer of a ceramic insulation layer and an electroluminescent layer, comprised of doped phosphors or the equivalent. One or more conductive top electrode layers having optically transmissive areas overlay the light emitting regions, and a multi-layered top barrier cover comprising one or more optically transmissive non-combustible insulation layers overlay said top electrode regions.

  14. Infrared emitting device and method

    DOEpatents

    Kurtz, Steven R.; Biefeld, Robert M.; Dawson, L. Ralph; Howard, Arnold J.; Baucom, Kevin C.

    1997-01-01

    An infrared emitting device and method. The infrared emitting device comprises a III-V compound semiconductor substrate upon which are grown a quantum-well active region having a plurality of quantum-well layers formed of a ternary alloy comprising InAsSb sandwiched between barrier layers formed of a ternary alloy having a smaller lattice constant and a larger energy bandgap than the quantum-well layers. The quantum-well layers are preferably compressively strained to increase the threshold energy for Auger recombination; and a method is provided for determining the preferred thickness for the quantum-well layers. Embodiments of the present invention are described having at least one cladding layer to increase the optical and carrier confinement in the active region, and to provide for waveguiding of the light generated within the active region. Examples have been set forth showing embodiments of the present invention as surface- and edge-emitting light emitting diodes (LEDs), an optically-pumped semiconductor laser, and an electrically-injected semiconductor diode laser. The light emission from each of the infrared emitting devices of the present invention is in the midwave infrared region of the spectrum from about 2 to 6 microns.

  15. Efficiency transfer using the GEANT4 code of CERN for HPGe gamma spectrometry.

    PubMed

    Chagren, S; Tekaya, M Ben; Reguigui, N; Gharbi, F

    2016-01-01

    In this work we apply the GEANT4 code of CERN to calculate the peak efficiency in High Pure Germanium (HPGe) gamma spectrometry using three different procedures. The first is a direct calculation. The second corresponds to the usual case of efficiency transfer between two different configurations at constant emission energy assuming a reference point detection configuration and the third, a new procedure, consists on the transfer of the peak efficiency between two detection configurations emitting the gamma ray in different energies assuming a "virtual" reference point detection configuration. No pre-optimization of the detector geometrical characteristics was performed before the transfer to test the ability of the efficiency transfer to reduce the effect of the ignorance on their real magnitude on the quality of the transferred efficiency. The obtained and measured efficiencies were found in good agreement for the two investigated methods of efficiency transfer. The obtained agreement proves that Monte Carlo method and especially the GEANT4 code constitute an efficient tool to obtain accurate detection efficiency values. The second investigated efficiency transfer procedure is useful to calibrate the HPGe gamma detector for any emission energy value for a voluminous source using one point source detection efficiency emitting in a different energy as a reference efficiency. The calculations preformed in this work were applied to the measurement exercise of the EUROMET428 project. A measurement exercise where an evaluation of the full energy peak efficiencies in the energy range 60-2000 keV for a typical coaxial p-type HpGe detector and several types of source configuration: point sources located at various distances from the detector and a cylindrical box containing three matrices was performed. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Unveiling the Nature of Soft Gamma Repeaters and Magnetars: Scientists Measure the Most Powerful Magnet Known

    NASA Technical Reports Server (NTRS)

    Swank, Jean (Technical Monitor); Parke, William

    2002-01-01

    This newsletter from NASA Goddard Space Flight Center (GSFC) announces measurements of the magnetic field of a magnetar. The magnetic field was approx. 10(exp 15) gauss, up to 10 times more powerful than previous estimates. The newsletter also describes how the star's magnetic field slows its rotation, and how starquakes emit protons, which are trapped in this neutron star's magnetic field, and make it a soft gamma repeater (SGR).

  17. 3D-printed focused collimator for intra-operative gamma-ray detection

    NASA Astrophysics Data System (ADS)

    Holdsworth, David W.; Nikolov, Hristo N.; Pollmann, Steven I.

    2017-03-01

    Recent developments in targeted radiopharmaceutical labels have increased the need for sensitive, real-time gamma detection during cancer surgery and biopsy. Additive manufacturing (3D printing) in metal has now made it possible to design and fabricate complex metal collimators for compact gamma probes. We describe the design and implementation of a 3D-printed focused collimator that allows for real-time detection of gamma radiation from within a small volume of interest, using a single-crystal large-area detector. The collimator was fabricated using laser melting of powdered stainless steel (316L), using a commercial 3D metal printer (AM125, Renishaw plc). The prototype collimator is 20 mm thick, with hexagonal close-packed holes designed to focus to a point 35 mm below the surface of the collimator face. Tests were carried out with a low-activity (<1 μCi) 241 Am source, using a conventional gamma-ray detector probe, incorporating a 2.5 cm diameter, 2.5 cm thick NaI crystal coupled to a photomultiplier. The measured full-width half maximum (FWHM) was less than 5.6 mm, and collimator detection efficiency was 44%. The ability to fabricate fine features in solid metal makes it possible to develop optimized designs for high-efficiency, focused gamma collimators for real-time intraoperative imaging applications.

  18. A cost-effective monitoring technique in particle therapy via uncollimated prompt gamma peak integration

    NASA Astrophysics Data System (ADS)

    Krimmer, J.; Angellier, G.; Balleyguier, L.; Dauvergne, D.; Freud, N.; Hérault, J.; Létang, J. M.; Mathez, H.; Pinto, M.; Testa, E.; Zoccarato, Y.

    2017-04-01

    For the purpose of detecting deviations from the prescribed treatment during particle therapy, the integrals of uncollimated prompt gamma-ray timing distributions are investigated. The intention is to provide information, with a simple and cost-effective setup, independent from monitoring devices of the beamline. Measurements have been performed with 65 MeV protons at a clinical cyclotron. Prompt gamma-rays emitted from the target are identified by means of time-of-flight. The proton range inside the PMMA target has been varied via a modulator wheel. The measured variation of the prompt gamma peak integrals as a function of the modulator position is consistent with simulations. With detectors covering a solid angle of 25 msr (corresponding to a diameter of 3-4 in. at a distance of 50 cm from the beam axis) and 108 incident protons, deviations of a few per cent in the prompt gamma-ray count rate can be detected. For the present configuration, this change in the count rate corresponds to a 3 mm change in the proton range in a PMMA target. Furthermore, simulation studies show that a combination of the signals from multiple detectors may be used to detect a misplacement of the target. A different combination of these signals results in a precise number of the detected prompt gamma rays, which is independent on the actual target position.

  19. Radiation measurement above the lunar surface by Kaguya gamma-ray spectrometer

    NASA Astrophysics Data System (ADS)

    Hasebe, Nobuyuki; Nagaoka, Hiroshi; Kusano, Hiroki; Hareyama, Matoko; Ideguchi, Yusuke; Shimizu, Sota; Shibamura, Eido

    The lunar surface is filled with various ionizing radiations such as high energy galactic particles, albedo particles and secondary radiations of neutrons, gamma rays and other elementary particles. A high-resolution Kaguya Gamma-Ray Spectrometer (KGRS) was carried on the Japan’s lunar explorer SELENE (Kaguya), the largest lunar orbiter since the Apollo missions. The KGRS instrument employed, for the first time in lunar exploration, a high-purity Ge crystal to increase the identification capability of elemental gamma-ray lines. The Ge detector is surrounded by BGO and plastic counters as for anticoincidence shields. The KGRS measured gamma rays in the energy range from 200 keV to 13 MeV with high precision to determine the chemical composition of the lunar surface. It provided data on the abundance of major elements over the entire lunar surface. In addition to the gamma-ray observation by the KGRS, it successfully measured the global distribution of fast neutrons. In the energy spectra of gamma-rays observed by the KGRS, several saw-tooth- peaks of Ge are included, which are formed by the collision interaction of lunar fast neutrons with Ge atoms in the Ge crystal. With these saw-tooth-peaks analysis, global distribution of neutrons emitted from the lunara surface was successfully created, which was compared with the previous results obtained by Lunar Prospector neutron maps. Another anticoincidence counter, the plastic counter with 5 mm thickness, was used to veto radiation events mostly generated by charged particles. A single photomultiplier serves to count scintillation light from the plastic scintillation counter. The global map of counting rates observed by the plastic counter was also created, implying that the radiation counting rate implies the geological distribution, in spite that the plastic counter mostly measures high energy charged particles and energetic neutrons. These results are presented and discussed.

  20. Fermi LAT discovery of GeV gamma-ray emission from the young supernova remnan Cassiopeia A

    DOE PAGES

    Abdo, A. A.

    2010-01-27

    Here, we report on the first detection of GeV high-energy gamma-ray emission from a young supernova remnant (SNR) with the Large Area Telescope aboard the Fermi Gamma-ray Space Telescope. Our observations reveal a source with no discernible spatial extension detected at a significance level of 12.2σ above 500 MeV at a location that is consistent with the position of the remnant of the supernova explosion that occurred around 1680 in the Cassiopeia constellation—Cassiopeia A (Cas A). The gamma-ray flux and spectral shape of the source are consistent with a scenario in which the gamma-ray emission originates from relativistic particles acceleratedmore » in the shell of this remnant. The total content of cosmic rays (electrons and protons) accelerated in Cas A can be estimated as W CR sime (1-4) × 1049 erg thanks to the well-known density in the remnant assuming that the observed gamma ray originates in the SNR shell(s). Finally, the magnetic field in the radio-emitting plasma can be robustly constrained as B ≥ 0.1 mG, providing new evidence of the magnetic field amplification at the forward shock and the strong field in the shocked ejecta.« less

  1. Constraining the location of gamma-ray flares in luminous blazars

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Nalewajko, Krzysztof; Begelman, Mitchell C.; Sikora, Marek, E-mail: knalew@jila.colorado.edu

    2014-07-10

    Locating the gamma-ray emission sites in blazar jets is a long standing and highly controversial issue. We jointly investigate several constraints on the distance scale r and Lorentz factor Γ of the gamma-ray emitting regions in luminous blazars (primarily flat spectrum radio quasars). Working in the framework of one-zone external radiation Comptonization models, we perform a parameter space study for several representative cases of actual gamma-ray flares in their multiwavelength context. We find a particularly useful combination of three constraints: from an upper limit on the collimation parameter Γθ ≲ 1, from an upper limit on the synchrotron self-Compton (SSC)more » luminosity L{sub SSC} ≲ L{sub X}, and from an upper limit on the efficient cooling photon energy E{sub cool,obs} ≲ 100 MeV. These three constraints are particularly strong for sources with low accretion disk luminosity L{sub d}. The commonly used intrinsic pair-production opacity constraint on Γ is usually much weaker than the SSC constraint. The SSC and cooling constraints provide a robust lower limit on the collimation parameter Γθ ≳ 0.1-0.7. Typical values of r corresponding to moderate values of Γ ∼ 20 are in the range 0.1-1 pc, and are determined primarily by the observed variability timescale t{sub var,obs}. Alternative scenarios motivated by the observed gamma-ray/millimeter connection, in which gamma-ray flares of t{sub var,obs} ∼ a few days are located at r ∼ 10 pc, are in conflict with both the SSC and cooling constraints. Moreover, we use a simple light travel time argument to point out that the gamma-ray/millimeter connection does not provide a significant constraint on the location of gamma-ray flares. We argue that spine-sheath models of the jet structure do not offer a plausible alternative to external radiation fields at large distances; however, an extended broad-line region is an idea worth exploring. We propose that the most definite additional constraint

  2. Prompt fission gamma-ray emission spectral data for 239Pu(n,f) using fast directional neutrons from the LICORNE neutron source

    NASA Astrophysics Data System (ADS)

    Qi, L.; Wilson, J. N.; Lebois, M.; Al-Adili, A.; Chatillon, A.; Choudhury, D.; Gatera, A.; Georgiev, G.; Göök, A.; Laurent, B.; Maj, A.; Matea, I.; Oberstedt, A.; Oberstedt, S.; Rose, S. J.; Schmitt, C.; Wasilewska, B.; Zeiser, F.

    2018-03-01

    Prompt fission gamma-ray spectra (PFGS) have been measured for the 239Pu(n,f) reaction using fast neutrons at Ēn=1.81 MeV produced by the LICORNE directional neutron source. The setup makes use of LaBr3 scintillation detectors and PARIS phoswich detectors to measure the emitted prompt fission gamma rays (PFG). The mean multiplicity, average total energy release per fission and average energy of photons are extracted from the unfolded PFGS. These new measurements provide complementary information to other recent work on thermal neutron induced fission of 239Pu and spontaneous fission of 252Cf.

  3. Feasibility study of single photon emission coupled tomography imaging technique based on prompt gamma ray during antiproton therapy using boron particle

    NASA Astrophysics Data System (ADS)

    Shin, Han-Back; Jung, Joo-Young; Kim, Moo-Sub; Kim, Sunmi; Choi, Yong; Yoon, Do-Kun; Suh, Tae Suk

    2018-06-01

    In this study, we proposed an absorbed-dose monitoring technique using prompt gamma rays emitted from the reaction between an antiproton and a boron particle, and demonstrated the greater physical effect of the antiproton boron fusion therapy in comparison with proton beam using Monte Carlo simulation. The physical effect of the treatment, which was 3.5 times greater, was confirmed from the antiproton beam irradiation compared to the proton beam irradiation. Moreover, the prompt gamma ray image is acquired successfully during antiproton irradiation to boron regions. The results show the application feasibility of absorbed dose monitoring technique proposed in our study.

  4. Recent developments in white light emitting diodes

    NASA Astrophysics Data System (ADS)

    Lohe, P. P.; Nandanwar, D. V.; Belsare, P. D.; Moharil, S. V.

    2018-05-01

    In the recent years solid state lighting based on LEDs has revolutionized lighting technology. LEDs have many advantages over the conventional lighting based on fluorescent and incandescent lamps such as mercury free, high conversion efficiency of electrical energy into light, long lifetime reliability and ability to use with many types of devices. LEDs have emerged as a new potentially revolutionary technology that could save up to half of energy used for lighting applications. White LEDs would be the most important light source in the future, so much so that this aspect had been highlighted by the Nobel committee during the award of 2014 Nobel Prize for Physics. Recent advancement in the fabrication of GaN chip capable of emitting in blue and near UV region paved way for fabrication of white LED lamps. Mainly there are two approaches used for preparing white emitting solid state lamp. In the first approach blue light (λ=450 nm) emitted from the InGaN LED chip is partially absorbed by the YAG:Ce3+ phosphor coated on it and re-emitted as yellow fluorescence. A white light can be generated by the combination of blue + yellow emission bands. These lamps are already available. But they are suffering from major drawback that their Colour Rendering Index (CRI) is low. In the second approach, white LEDs are made by coating near ultraviolet emitting (360 to 410nm) LED with a mixture of high efficiency red, green and blue emitting phosphors, analogous to the fluorescent lamp. This method yields lamps with better color rendition. Addition of a yellow emitting phosphor improves CRI further. However conversion efficiency is compromised to some extent. Further the cost of near UV emitting chip is very high compared to blue emitting chips. Thus cost and light output wise, near UV chips are much inferior to blue chips. Recently some rare earth activated oxynitrides, silicates, fluorides have emerged as an important family of luminescent materials for white LED application

  5. An optical view of extragalactic gamma-ray emitters

    NASA Astrophysics Data System (ADS)

    Paiano, Simona; Falomo, Renato; Landoni, Marco; Treves, Aldo; Scarpa, Riccardo

    2017-11-01

    The Fermi Gamma-ray Observatory discovered about a thousand extragalactic sources emitting energy from 100 MeV to 100 GeV. The majority of these sources belong to the class of blazars characterized by a quasi-featureless optical spectrum (BL Lac Objects). This hampers the determination of their redshift and therefore hinders the characterization of this class of objects. To investigate the nature of these sources and to determine their redshift, we are carrying out an extensive campaign at the 10m Gran Telescopio Canarias to secure high signal-to-noise ratio optical spectra. These observations allow us to confirm the blazar nature of the targets, to find new redshifts or to set stringent limits on the redshift based on the minimum equivalent width of absorption features expected from their host galaxy, assuming it is a massive elliptical galaxy.These results are of importance for the multi-frequencies emission models of the blazars, to test their extreme physics, to shed light on their cosmic evolution and abundance in the far Universe.These gamma emitters are also of great importance for the characterization of the extragalactic background light through the absorption by the IR-optical background photons.

  6. Development of gamma-photon/Cerenkov-light hybrid system for simultaneous imaging of I-131 radionuclide

    NASA Astrophysics Data System (ADS)

    Yamamoto, Seiichi; Suzuki, Mayumi; Kato, Katsuhiko; Watabe, Tadashi; Ikeda, Hayato; Kanai, Yasukazu; Ogata, Yoshimune; Hatazawa, Jun

    2016-09-01

    Although iodine 131 (I-131) is used for radionuclide therapy, high resolution images are difficult to obtain with conventional gamma cameras because of the high energy of I-131 gamma photons (364 keV). Cerenkov-light imaging is a possible method for beta emitting radionuclides, and I-131 (606 MeV maximum beta energy) is a candidate to obtain high resolution images. We developed a high energy gamma camera system for I-131 radionuclide and combined it with a Cerenkov-light imaging system to form a gamma-photon/Cerenkov-light hybrid imaging system to compare the simultaneously measured images of these two modalities. The high energy gamma imaging detector used 0.85-mm×0.85-mm×10-mm thick GAGG scintillator pixels arranged in a 44×44 matrix with a 0.1-mm thick reflector and optical coupled to a Hamamatsu 2 in. square position sensitive photomultiplier tube (PSPMT: H12700 MOD). The gamma imaging detector was encased in a 2 cm thick tungsten shield, and a pinhole collimator was mounted on its top to form a gamma camera system. The Cerenkov-light imaging system was made of a high sensitivity cooled CCD camera. The Cerenkov-light imaging system was combined with the gamma camera using optical mirrors to image the same area of the subject. With this configuration, we simultaneously imaged the gamma photons and the Cerenkov-light from I-131 in the subjects. The spatial resolution and sensitivity of the gamma camera system for I-131 were respectively 3 mm FWHM and 10 cps/MBq for the high sensitivity collimator at 10 cm from the collimator surface. The spatial resolution of the Cerenkov-light imaging system was 0.64 mm FWHM at 10 cm from the system surface. Thyroid phantom and rat images were successfully obtained with the developed gamma-photon/Cerenkov-light hybrid imaging system, allowing direct comparison of these two modalities. Our developed gamma-photon/Cerenkov-light hybrid imaging system will be useful to evaluate the advantages and disadvantages of these two

  7. Development of computational pregnant female and fetus models and assessment of radiation dose from positron-emitting tracers.

    PubMed

    Xie, Tianwu; Zaidi, Habib

    2016-12-01

    Molecular imaging using PET and hybrid (PET/CT and PET/MR) modalities nowadays plays a pivotal role in the clinical setting for diagnosis and staging, treatment response monitoring, and radiation therapy treatment planning of a wide range of oncologic malignancies. The developing embryo/fetus presents a high sensitivity to ionizing radiation. Therefore, estimation of the radiation dose delivered to the embryo/fetus and pregnant patients from PET examinations to assess potential radiation risks is highly praised. We constructed eight embryo/fetus models at various gestation periods with 25 identified tissues according to reference data recommended by the ICRP publication 89 representing the anatomy of the developing embryo/fetus. The developed embryo/fetus models were integrated into realistic anthropomorphic computational phantoms of the pregnant female and used for estimating, using Monte Carlo calculations, S-values of common positron-emitting radionuclides, organ absorbed dose, and effective dose of a number of positron-emitting labeled radiotracers. The absorbed dose is nonuniformly distributed in the fetus. The absorbed dose of the kidney and liver of the 8-week-old fetus are about 47.45 % and 44.76 % higher than the average absorbed dose of the fetal total body for all investigated radiotracers. For 18 F-FDG, the fetal effective doses are 2.90E-02, 3.09E-02, 1.79E-02, 1.59E-02, 1.47E-02, 1.40E-02, 1.37E-02, and 1.27E-02 mSv/MBq at the 8th, 10th, 15th, 20th, 25th, 30th, 35th, and 38th weeks of gestation, respectively. The developed pregnant female/fetus models matching the ICRP reference data can be exploited by dedicated software packages for internal and external dose calculations. The generated S-values will be useful to produce new standardized dose estimates to pregnant patients and embryo/fetus from a variety of positron-emitting labeled radiotracers.

  8. Limited tryptic proteolysis of the benzodiazepine binding proteins in different species reveals structural homologies.

    PubMed

    Friedl, W; Lentes, K U; Schmitz, E; Propping, P; Hebebrand, J

    1988-12-01

    Peptide mapping can be used to elucidate further the structural similarities of the benzodiazepine binding proteins in different vertebrate species. Crude synaptic membrane preparations were photoaffinity-labeled with [3H]flunitrazepam and subsequently degraded with various concentrations of trypsin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by fluorography allowed a comparison of the molecular weights of photolabeled peptides in different species. Tryptic degradation led to a common peptide of 40K in all species investigated, a finding indicating that the benzodiazepine binding proteins are structurally homologous in higher bony fishes and tetrapods.

  9. Efficient semiconductor light-emitting device and method

    DOEpatents

    Choquette, Kent D.; Lear, Kevin L.; Schneider, Jr., Richard P.

    1996-01-01

    A semiconductor light-emitting device and method. The semiconductor light-emitting device is provided with at least one control layer or control region which includes an annular oxidized portion thereof to channel an injection current into the active region, and to provide a lateral refractive index profile for index guiding the light generated within the device. A periodic composition grading of at least one of the mirror stacks in the device provides a reduced operating voltage of the device. The semiconductor light-emitting device has a high efficiency for light generation, and may be formed either as a resonant-cavity light-emitting diode (RCLED) or as a vertical-cavity surface-emitting laser (VCSEL).

  10. Efficient semiconductor light-emitting device and method

    DOEpatents

    Choquette, K.D.; Lear, K.L.; Schneider, R.P. Jr.

    1996-02-20

    A semiconductor light-emitting device and method are disclosed. The semiconductor light-emitting device is provided with at least one control layer or control region which includes an annular oxidized portion thereof to channel an injection current into the active region, and to provide a lateral refractive index profile for index guiding the light generated within the device. A periodic composition grading of at least one of the mirror stacks in the device provides a reduced operating voltage of the device. The semiconductor light-emitting device has a high efficiency for light generation, and may be formed either as a resonant-cavity light-emitting diode (RCLED) or as a vertical-cavity surface-emitting laser (VCSEL). 12 figs.

  11. Short-term variability of gamma radiation at the ARM Eastern North Atlantic facility (Azores).

    PubMed

    Barbosa, S M; Miranda, P; Azevedo, E B

    2017-06-01

    This work addresses the short-term variability of gamma radiation measured continuously at the Eastern North Atlantic (ENA) facility located in the Graciosa island (Azores, 39N; 28W), a fixed site of the Atmospheric Radiation Measurement programme (ARM). The temporal variability of gamma radiation is characterized by occasional anomalies over a slowly-varying signal. Sharp peaks lasting typically 2-4 h are coincident with heavy precipitation and result from the scavenging effect of precipitation bringing radon progeny from the upper levels to the ground surface. However the connection between gamma variability and precipitation is not straightforward as a result of the complex interplay of factors such as the precipitation intensity, the PBL height, the cloud's base height and thickness, or the air mass origin and atmospheric concentration of sub-micron aerosols, which influence the scavenging processes and therefore the concentration of radon progeny. Convective precipitation associated with cumuliform clouds forming under conditions of warming of the ground relative to the air does not produce enhancements in gamma radiation, since the drop growing process is dominated by the fast accretion of liquid water, resulting in the reduction of the concentration of radionuclides by dilution. Events of convective precipitation further contribute to a reduction in gamma counts by inhibiting radon release from the soil surface and by attenuating gamma rays from all gamma-emitting elements on the ground. Anomalies occurring in the absence of precipitation are found to be associated with a diurnal cycle of maximum gamma counts before sunrise decreasing to a minimum in the evening, which are observed in conditions of thermal stability and very weak winds enabling the build-up of near surface radon progeny during the night. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Cen A Radio Optical Gamma Composite

    NASA Image and Video Library

    2017-12-08

    NASA release April 1, 2010 It takes the addition of radio data (orange) to fully appreciate the scale of Cen A's giant radio-emitting lobes, which stretch more than 1.4 million light-years. Gamma-rays from Fermi's Large Area Telescope (purple) and an image of the galaxy in visible light are also included in this composite. Credit: NASA/DOE/Fermi LAT Collaboration, Capella Observatory, and Ilana Feain, Tim Cornwell, and Ron Ekers (CSIRO/ATNF), R. Morganti (ASTRON), and N. Junkes (MPIfR) To learn more about these images go to: www.nasa.gov/mission_pages/GLAST/news/smokestack-plumes.html NASA Goddard Space Flight Center is home to the nation's largest organization of combined scientists, engineers and technologists that build spacecraft, instruments and new technology to study the Earth, the sun, our solar system, and the universe.

  13. An open-label tolerability study of BL-1020 antipsychotic: a novel gamma aminobutyric acid ester of perphenazine.

    PubMed

    Anand, Ravi; Geffen, Yona; Vasile, Daniel; Dan, Irina

    2010-01-01

    BL-1020, a novel gamma aminobutyric acid (GABA) ester of perphenazine, is a new oral antipsychotic with a strong affinity for dopamine and serotonin receptors. Unlike first- and second-generation antipsychotics, it has agonist activity at GABA(A). This is the first study to examine tolerability and safety of BL-1020 in schizophrenia. This was a phase-II, open-label, multicenter, 6-week study treating patients (n = 36) with chronic schizophrenia. Dosing started at 20 mg/d and increased over 7 days to 40 mg/d. Weekly assessments were conducted. All but 1 patient was titrated to 30 mg/d at day 4; on day 7, 30 were titrated to 40 mg/d. Four patients discontinued the study prematurely. There was no clinically relevant increase in vital signs, sedation, dizziness, or other central nervous system effects or electrocardiogram or laboratory abnormalities and a small increase in weight. Ten patients experienced extrapyramidal symptoms (EPS) requiring treatment with an anticholinergic; 4 patients were unable to reach maximum dose because of EPS. Extrapyramidal Symptom Rating Scale did not indicate clinically significant changes in EPS. The most common adverse event was insomnia (6 patients); other frequent adverse effects (all n = 3) were extrapyramidal disorder, headache, parkinsonism, tremor, and hyperprolactinemia. There was improvement on Positive and Negative Syndrome Scale and Clinical Global Impression of Change with 22 patients showing at least 20% decrease by end point on Positive and Negative Syndrome Scale and 31 patients showing at least minimal improvement on Clinical Global Impression of Change. These data suggest that 20 to 40 mg/d of BL-1020 is associated with clinically relevant improvement of psychosis with no worsening of EPS and support further testing in randomized controlled trials.

  14. Expected gamma-ray emission spectra from the lunar surface as a function of chemical composition

    NASA Technical Reports Server (NTRS)

    Reedy, R. C.; Arnold, J. R.; Trombka, J. I.

    1973-01-01

    The gamma rays emitted from the moon or any similar body carry information on the chemical composition of the surface layer. The elements most easily measured are K, U, Th and major elements such as O, Si, Mg, and Fe. The expected fluxes of gamma ray lines were calculated for four lunar compositions and one chondritic chemistry from a consideration of the important emission mechanisms: natural radioactivity, inelastic scatter, neutron capture, and induced radioactivity. The models used for cosmic ray interactions were those of Reedy and Arnold and Lingenfelter. The areal resolution of the experiment was calculated to be around 70 to 140 km under the conditions of the Apollo 15 and 16 experiments. Finally, a method was described for recovering the chemical information from the observed scintillation spectra obtained in these experiments.

  15. Light-emitting device with organic electroluminescent material and photoluminescent materials

    DOEpatents

    McNulty, Thomas Francis; Duggal, Anil Raj; Turner, Larry Gene; Shiang, Joseph John

    2005-06-07

    A light-emitting device comprises a light-emitting member, which comprises two electrodes and an organic electroluminescent material disposed between the electrodes, and at least one organic photoluminescent ("PL") material. The light-emitting member emits light having a first spectrum in response to a voltage applied across the two electrodes. The organic PL material absorbs a portion of the light emitted by the light-emitting member and emits light having second spectrum different than the first spectrum. The light-emitting device can include an inorganic PL material that absorbs another portion of the light emitted from the light-emitting member and emits light having a third spectrum different than both the first and the second spectra.

  16. Gamma camera dual imaging with a somatostatin receptor and thymidine kinase after gene transfer with a bicistronic adenovirus in mice.

    PubMed

    Zinn, Kurt R; Chaudhuri, Tandra R; Krasnykh, Victor N; Buchsbaum, Donald J; Belousova, Natalya; Grizzle, William E; Curiel, David T; Rogers, Buck E

    2002-05-01

    To compare two systems for assessing gene transfer to cancer cells and xenograft tumors with noninvasive gamma camera imaging. A replication-incompetent adenovirus encoding the human type 2 somatostatin receptor (hSSTr2) and the herpes simplex virus thymidine kinase (TK) enzyme (Ad-hSSTr2-TK) was constructed. A-427 human lung cancer cells were infected in vitro and mixed with uninfected cells at different ratios. A-427 tumors in nude mice (n = 23) were injected with 1 x 10(6) to 5 x 10(8) plaque-forming units (pfu) of Ad-hSSTr2-TK. The expressed hSSTr2 and TK proteins were imaged owing to internally bound, or trapped, technetium 99m ((99m)Tc)-labeled hSSTr2-binding peptide (P2045) and radioiodinated 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-5-iodouracil (FIAU), respectively. Iodine 125 ((125)I)-labeled FIAU was used in vitro and iodine 131 ((131)I)-labeled FIAU, in vivo. The (99m)Tc-labeled P2045 and (125)I- or (131)I-labeled FIAU were imaged simultaneously with different window settings with an Anger gamma camera. Treatment effects were tested with analysis of variance. Infected cells in culture trapped (125)I-labeled FIAU and (99m)Tc-labeled P2045; uptake correlated with the percentage of Ad-hSSTr2-TK-positive cells. For 100% of infected cells, 24% +/- 0.4 (mean +/- SD) of the added (99m)Tc-labeled P2045 was trapped, which is significantly lower (P <.05) than the 40% +/- 2 of (125)I-labeled FIAU that was trapped. For the highest Ad-hSSTr2-TK tumor dose (5 x 10(8) pfu), the uptake of (99m)Tc-labeled P2045 was 11.1% +/- 2.9 of injected dose per gram of tumor (thereafter, dose per gram), significantly higher (P <.05) than the uptake of (131)I-labeled FIAU at 1.6% +/- 0.4 dose per gram. (99m)Tc-labeled P2045 imaging consistently depicted hSSTr2 gene transfer in tumors at all adenovirus doses. Tumor uptake of (99m)Tc-labeled P2045 positively correlated with Ad-hSSTr2-TK dose; (131)I-labeled FIAU tumor uptake did not correlate with vector dose. The hSSTr2 and TK

  17. Detection of gamma-irradiation effect on DNA and protein using magnetic sensor and cyclic voltammetry.

    PubMed

    Park, Duck-Gun; Song, Hoon; Kishore, M B; Vértesy, G; Lee, Duk-Hyun

    2013-11-01

    In this study, a magnetic sensor utilizing Planar Hall Resistance (PHR) and cyclic Voltammetry (CV) for detecting the radiation effect was fabricated. Specifically, we applied in parallel a PHR sensor and CV device to monitor the irradiation effect on DNA and protein respectively. Through parallel measurements, we demonstrated that the PHR sensor and CV are sensitive enough to measure irradiation effect. The PHR voltage decreased by magnetic nanobead labeled DNA was slightly recovered after gamma ray irradiation. The behavior of cdk inhibitor protein p21 having a sandwich structure of Au/protein G/Ab/Ag/Ab was checked by monitoring the cyclic Voltammetry signal in analyzing the gamma ray irradiation effect.

  18. Prompt gamma timing range verification for scattered proton beams

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kormoll, T.; Golnik, C.; Hueso Gonzalez, F.

    2015-07-01

    Range verification is a very important point in order to fully exploit the physical advantages of protons compared to photons in cancer irradiation. Recently, a simple method has been proposed which makes use of the time of fight of protons in tissue and the promptly emitted secondary photons along the proton path (Prompt Gamma Timing, PGT). This has been considered so far for monoenergetic pencil beams only. In this work, it has been studied whether this technique can also be applied in passively formed irradiation fields with a so called spread out Bragg peak. Time correlated profiles could be recorded,more » which show a trend that is consistent with theoretical predictions. (authors)« less

  19. Diffuse Gamma Rays Galactic and Extragalactic Diffuse Emission

    NASA Technical Reports Server (NTRS)

    Moskalenko, Igor V.; Strong, Andrew W.; Reimer, Olaf

    2004-01-01

    Diffuse gamma rays consist of several components: truly diffuse emission from the interstellar medium, the extragalactic background, whose origin is not firmly established yet, and the contribution from unresolved and faint Galactic point sources. One approach to unravel these components is to study the diffuse emission from the interstellar medium, which traces the interactions of high energy particles with interstellar gas and radiation fields. Because of its origin such emission is potentially able to reveal much about the sources and propagation of cosmic rays. The extragalactic background, if reliably determined, can be used in cosmological and blazar studies. Studying the derived average spectrum of faint Galactic sources may be able to give a clue to the nature of the emitting objects.

  20. Broadband light-emitting diode

    DOEpatents

    Fritz, Ian J.; Klem, John F.; Hafich, Michael J.

    1998-01-01

    A broadband light-emitting diode. The broadband light-emitting diode (LED) comprises a plurality of III-V compound semiconductor layers grown on a semiconductor substrate, with the semiconductor layers including a pair of cladding layers sandwiched about a strained-quantum-well active region having a plurality of different energy bandgaps for generating light in a wavelength range of about 1.3-2 .mu.m. In one embodiment of the present invention, the active region may comprise a first-grown quantum-well layer and a last-grown quantum-well layer that are oppositely strained; whereas in another embodiment of the invention, the active region is formed from a short-period superlattice structure (i.e. a pseudo alloy) comprising alternating thin layers of InGaAs and InGaAlAs. The use a short-period superlattice structure for the active region allows different layers within the active region to be simply and accurately grown by repetitively opening and closing one or more shutters in an MBE growth apparatus to repetitively switch between different growth states therein. The broadband LED may be formed as either a surface-emitting LED or as an edge-emitting LED for use in applications such as chemical sensing, fiber optic gyroscopes, wavelength-division-multiplexed (WDM) fiber-optic data links, and WDM fiber-optic sensor networks for automobiles and aircraft.

  1. Broadband light-emitting diode

    DOEpatents

    Fritz, I.J.; Klem, J.F.; Hafich, M.J.

    1998-07-14

    A broadband light-emitting diode is disclosed. The broadband light-emitting diode (LED) comprises a plurality of III-V compound semiconductor layers grown on a semiconductor substrate, with the semiconductor layers including a pair of cladding layers sandwiched about a strained-quantum-well active region having a plurality of different energy bandgaps for generating light in a wavelength range of about 1.3--2 {micro}m. In one embodiment of the present invention, the active region may comprise a first-grown quantum-well layer and a last-grown quantum-well layer that are oppositely strained; whereas in another embodiment of the invention, the active region is formed from a short-period superlattice structure (i.e. a pseudo alloy) comprising alternating thin layers of InGaAs and InGaAlAs. The use a short-period superlattice structure for the active region allows different layers within the active region to be simply and accurately grown by repetitively opening and closing one or more shutters in an MBE growth apparatus to repetitively switch between different growth states therein. The broadband LED may be formed as either a surface-emitting LED or as an edge-emitting LED for use in applications such as chemical sensing, fiber optic gyroscopes, wavelength-divisionmultiplexed (WDM) fiber-optic data links, and WDM fiber-optic sensor networks for automobiles and aircraft. 10 figs.

  2. The effects of gamma radiation, UV and visible light on ATP levels in yeast cells depend on cellular melanization.

    PubMed

    Bryan, Ruth; Jiang, Zewei; Friedman, Matthew; Dadachova, Ekaterina

    2011-10-01

    Previously we have shown that growth of melanized fungi is stimulated by low levels of gamma radiation. The goal of this study was to examine the effects of visible light, UV light, and gamma radiation on the energy level (ATP concentration) in melanized Cryptococcus neoformans cells. Melanized C. neoformans cells as well as non-melanized controls were subjected to visible, UV or gamma radiation, and ATP was quantified by measuring the amount of light emitted by the ATP-dependent reaction of luciferase with luciferin. We found that all three forms of radiation led to a reduction in the ATP levels in melanized C. neoformans cells. This points to a universal melanin-related mechanism underlying observation of ATP decrease in irradiated melanized cells. In contrast, in non-melanized cells visible light led to increase in ATP levels; gamma radiation did not cause any changes while UV exposure resulted in some ATP decrease, however, much less pronounced than in melanized cells. Copyright © 2011 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

  3. Biogenic volatile organic compounds (BVOCs) emission of Scots pine under drought stress - a 13CO2 labeling study to determine de novo and pool emissions under different treatments

    NASA Astrophysics Data System (ADS)

    Lüpke, M.

    2015-12-01

    Plants emit biogenic volatile organic compounds (BVOCs) to e.g. communicate and to defend herbivores. Yet BVOCs also impact atmospheric chemistry processes, and lead to e.g. the built up of secondary organic aerosols. Abiotic stresses, such as drought, however highly influence plant physiology and subsequently BVOCs emission rates. In this study, we investigated the effect of drought stress on BVOCs emission rates of Scots pine trees, a de novo and pool emitter, under controlled climate chamber conditions within a dynamic enclosure system consisting of four plant chambers. Isotopic labeling with 13CO2 was used to detect which ratio of emissions of BVOCs derives from actual synthesis and from storage organs under different treatments. Additionally, the synthesis rate of the BVOCs synthesis can be determined. The experiment consisted of two campaigns (July 2015 and August 2015) of two control and two treated trees respectively in four controlled dynamic chambers simultaneously. Each campaign lasted for around 21 days and can be split into five phases: adaptation, control, dry-out, drought- and re-watering phase. The actual drought phase lasted around five days. During the campaigns two samples of BVOCs emissions were sampled per day and night on thermal desorption tubes and analyzed by a gas chromatograph coupled with a mass spectrometer and a flame ionization detector. Additionally, gas exchange of water and CO2, soil moisture, as well as leaf and chamber temperature was monitored continuously. 13CO2 labeling was performed simultaneously in all chambers during the phases control, drought and re-watering for five hours respectively. During the 13CO2 labeling four BVOCs emission samples per chamber were taken to identify the labeling rate on emitted BVOCs. First results show a decrease of BVOCs emissions during the drought phase and a recovery of emission after re-watering, as well as different strength of reduction of single compounds. The degree of labeling with 13

  4. Long lifetime near-infrared-emitting quantum dots for time-gated in vivo imaging of rare circulating cells (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Fragola, Alexandra; Bouccara, Sophie; Pezet, Sophie; Lequeux, Nicolas; Loriette, Vincent; Pons, Thomas

    2017-02-01

    The in vivo detection of rare circulating cells using non invasive fluorescence imaging would provide a key tool to study migration of eg. tumoral or immunological cells. Fluorescence detection is however currently limited by a lack of contrast between the small emission of isolated, fast circulating cells and the strong autofluorescence background of the surrounding tissues. We present the development of near infrared emitting quantum dots (NIR-QDs) with long fluorescence lifetime for sensitive time-gated in vivo imaging of circulating cells. These QDs are composed of low toxicity ZnCuInSe/ZnS materials and made biocompatible using a novel multidentate imidazole zwitterionic block copolymer, ensuring their long term intracellular stability. Cells of interest can thus be labeled ex vivo with QDs, injected intravenously and imaged in the near infrared range. Excitation using a pulsed laser coupled to time-gated detection enables the efficient rejection of short lifetime (≈ ns) autofluorescence background and detection of long lifetime (≈ 150 ns) fluorescence from QD-labeled cells. We demonstrate efficient in vivo imaging of single fast-flowing cells, which opens opportunities for future biological studies. [1] M. Tasso et al, "Sulfobetaine-Vinylimidazole block copolymers: a robust quantum dot surface chemistry expanding bioimaging's horizons", ACS Nano, 9(11), 2015 [2] S. Bouccara et al, "Time-gated cell imaging using long lifetime near-infrared-emitting quantum dots for autofluorescence rejection", J Biomed Optc, 19(5), 2014

  5. Rapid production of positron emitting labeled compounds for use in cardiology PET studies

    NASA Astrophysics Data System (ADS)

    Bolomey, Leonard

    1985-05-01

    Large scale clinical application of positron emission tomography requires a variety of short-lived positron emitting radionuclides to be produced in Curie quantities up to 20 times per day. Rapid routine production of these radiopharmaceuticals requires the collaboration of engineers and chemists to achieve production targetry compatible with high beam current (up to 100 μA) and radionuclide production in a chemical form compatible with the rapid radiochemical synthesis. Chemical processing is further complicated by the need to repeat the procedures several times per day and maintain sterility within the shielded area. At our cyclotron facility primary production targets for 11C, 13N, 15O, and 18F (half lives from 2 min to 2 h) are mounted on a vertical gantr that indexes to position the required target on the beam line. Target changes are handled under microprocessor control remotely from the control room such that all valves, cooling, evacuation of target manifold, and testing of interlocks are handled automatically. This system enables us to change targets, energy and particles in less than five minutes. Since the installation of the cyclotron up to fifteen batches of routine radiopharmaceuticals have been produced per day with very low radiation doses to all personnel involved. These radiopharmaceuticals will be used to measure perfusion, metabolism and other biochemical functions in man non invasively with PET.

  6. Prompt optical emission from gamma-ray bursts with multiple timescale variability of central engine activities

    NASA Astrophysics Data System (ADS)

    Xu, Si-Yao; Li, Zhuo

    2014-04-01

    Complete high-resolution light curves of GRB 080319B observed by Swift present an opportunity for detailed temporal analysis of prompt optical emission. With a two-component distribution of initial Lorentz factors, we simulate the dynamical process of shells being ejected from the central engine in the framework of the internal shock model. The emitted radiations are decomposed into different frequency ranges for a temporal correlation analysis between the light curves in different energy bands. The resulting prompt optical and gamma-ray emissions show similar temporal profiles, with both showing a superposition of a component with slow variability and a component with fast variability, except that the gamma-ray light curve is much more variable than its optical counterpart. The variability in the simulated light curves and the strong correlation with a time lag between the optical and gamma-ray emissions are in good agreement with observations of GRB 080319B. Our simulations suggest that the variations seen in the light curves stem from the temporal structure of the shells injected from the central engine of gamma-ray bursts. Future observations with high temporal resolution of prompt optical emission from GRBs, e.g., by UFFO-Pathfinder and SVOM-GWAC, will provide a useful tool for investigating the central engine activity.

  7. Binary Orbits as the Driver of Gamma-Ray Emission and Mass Ejection in Classical Novae

    NASA Technical Reports Server (NTRS)

    Chomiuk, Laura; Linford, Justin D.; Yang, Jun; O'Brien, T. J.; Paragi, Zsolt; Mioduszewski, Amy J.; Beswick, R. J.; Cheung, C. C.; Mukai, Koji; Nelson, Thomas

    2014-01-01

    Classical novae are the most common astrophysical thermonuclear explosions, occurring on the surfaces of white dwarf stars accreting gas from companions in binary star systems. Novae typically expel about 10 (sup -4) solar masses of material at velocities exceeding 1,000 kilometers per second.However, the mechanism of mass ejection in novae is poorly understood, and could be dominated by the impulsive flash of thermonuclear energy, prolonged optically thick winds or binary interaction with the nova envelope. Classical novae are now routinely detected at giga-electronvolt gamma-ray wavelengths, suggesting that relativistic particles are accelerated by strong shocks in the ejecta. Here we report high-resolution radio imaging of the gamma-ray-emitting nova V959 Mon. We find that its ejecta were shaped by the motion of the binary system: some gas was expelled rapidly along the poles as a wind from the white dwarf, while denser material drifted out along the equatorial plane, propelled by orbital motion..At the interface between the equatorial and polar regions, we observe synchrotron emission indicative of shocks and relativistic particle acceleration, thereby pinpointing the location of gamma-ray production. Binary shaping of the nova ejecta and associated internal shocks are expected to be widespread among novae, explaining why many novae are gamma-ray emitters.

  8. gamma-Hexachlorocyclohexane (gamma-HCH)

    Integrated Risk Information System (IRIS)

    gamma - Hexachlorocyclohexane ( gamma - HCH ) ; CASRN 58 - 89 - 9 Human health assessment information on a chemical substance is included in the IRIS database only after a comprehensive review of toxicity data , as outlined in the IRIS assessment development process . Sections I ( Health Hazard Asse

  9. Comparative Mg(2+)-dependent sequential covalent binding stoichiometries of 3'-O-(4-benzoyl)benzoyl adenosine 5'-diphosphate of MF1, TF1, and the alpha 3 beta 3 core complex of TF1. The binding change motif is independent of the F1 gamma delta epsilon subunits.

    PubMed

    Aloise, P; Kagawa, Y; Coleman, P S

    1991-06-05

    Three F1 preparations, the beef heart (MF1) and thermophilic bacterium (TF1) holoenzymes, and the alpha 3 beta 3 "core" complex of TF1 reconstituted from individually expressed alpha and beta subunits, were compared as to their kinetic and binding stoichiometric responses to covalent photoaffinity labeling with BzATP and BzADP (+/- Mg2+). Each enzyme displayed an enhanced pseudo-first order rate of photoinhibition and one-third of the sites covalent binding to a catalytic site for full inhibition, plus, but not minus Mg2+. Titration of near stoichiometric [MgBzADP]/[F1] ratios during photolysis disclosed two sequential covalent binding patterns for each enzyme; a high affinity binding corresponding to unistoichiometric covalent association concomitant with enzyme inhibition, followed by a low affinity multisite-saturating covalent association. Thus, in the absence of the structural asymmetry inducing gamma delta epsilon subunits of the holoenzyme, the sequential binding of nucleotide at putative catalytic sites on the alpha 3 beta 3 complex of any F1 appears sufficient to effect binding affinity changes. With MF1, final covalent saturation of BzADP-accessible sites was achieved with 2 mol of BzADP/mol of enzyme, but with TF1 or its alpha 3 beta 3 complex, saturation required 3 mol of BzADP/mol of enzyme. Such differential final labeling stoichiometries could arise because of the endogenous presence of 1 nucleotide already bound to one of the 3 potential catalytic sites on normally prepared MF1, whereas TF1, possessing no endogenous nucleotide, has 3 vacant BzADP-accessible sites. Kinetics measurements revealed that regardless of the incremental extent of inhibition of the TF1 holoenzyme by BzADP during photolysis, the two higher apparent Km values (approximately 1.5 x 10(-4) and approximately 10(-3) M, respectively) of the progressively inactivated incubation are unchanged relative to fully unmodified enzyme. As reported for BzATP (or BzADP) and MF1 (Ackerman, S

  10. Applying the new gamma ray imager diagnostic to measurements of runaway electron Bremsstrahlung radiation in the DIII-D Tokamak (invited)

    NASA Astrophysics Data System (ADS)

    Cooper, C. M.; Pace, D. C.; Paz-Soldan, C.; Commaux, N.; Eidietis, N. W.; Hollmann, E. M.; Shiraki, D.

    2016-11-01

    A new gamma ray imager (GRI) is developed to probe the electron distribution function with 2D spatial resolution during runaway electron (RE) experiments at the DIII-D tokamak. The diagnostic is sensitive to 0.5-100 MeV gamma rays, allowing characterization of the RE distribution function evolution during RE growth and dissipation. The GRI consists of a lead "pinhole camera" mounted on the DIII-D midplane with 123 honeycombed tangential chords 20 cm wide that span the vessel interior. Up to 30 bismuth germanate (BGO) scintillation detectors capture RE bremsstrahlung radiation for Pulse Height Analysis (PHA) capable of discriminating up to 20 000 pulses per second. Digital signal processing routines combining shaping filters are performed during PHA to reject noise and record gamma ray energy. The GRI setup and PHA algorithms will be described and initial data from experiments will be presented. A synthetic diagnostic is developed to generate the gamma ray spectrum of a GRI channel given the plasma information and a prescribed distribution function. Magnetic reconstructions of the plasma are used to calculate the angle between every GRI sightline and orient and discriminate gamma rays emitted by a field-aligned RE distribution function.

  11. Applying the new gamma ray imager diagnostic to measurements of runaway electron Bremsstrahlung radiation in the DIII-D Tokamak (invited).

    PubMed

    Cooper, C M; Pace, D C; Paz-Soldan, C; Commaux, N; Eidietis, N W; Hollmann, E M; Shiraki, D

    2016-11-01

    A new gamma ray imager (GRI) is developed to probe the electron distribution function with 2D spatial resolution during runaway electron (RE) experiments at the DIII-D tokamak. The diagnostic is sensitive to 0.5-100 MeV gamma rays, allowing characterization of the RE distribution function evolution during RE growth and dissipation. The GRI consists of a lead "pinhole camera" mounted on the DIII-D midplane with 123 honeycombed tangential chords 20 cm wide that span the vessel interior. Up to 30 bismuth germanate (BGO) scintillation detectors capture RE bremsstrahlung radiation for Pulse Height Analysis (PHA) capable of discriminating up to 20 000 pulses per second. Digital signal processing routines combining shaping filters are performed during PHA to reject noise and record gamma ray energy. The GRI setup and PHA algorithms will be described and initial data from experiments will be presented. A synthetic diagnostic is developed to generate the gamma ray spectrum of a GRI channel given the plasma information and a prescribed distribution function. Magnetic reconstructions of the plasma are used to calculate the angle between every GRI sightline and orient and discriminate gamma rays emitted by a field-aligned RE distribution function.

  12. The use of ionic salt dyes as amorphous, thermally stable emitting layers in organic light-emitting diodes

    NASA Astrophysics Data System (ADS)

    Chondroudis, Konstantinos; Mitzi, David B.

    2000-01-01

    The conversion of two neutral dye molecules (D) to ionic salts (H2N-D-NH2ṡ2HX) and their utilization as emitting layers in organic light-emitting diodes (OLEDs) is described. The dye salts, AEQTṡ2HCl and APTṡ2HCl, can be deposited as amorphous films using conventional evaporation techniques. X-ray diffraction and scanning electron microscopy analysis, coupled with thermal annealing studies, demonstrate the resistance of the films to crystallization. This stability is attributed to strong ionic forces between the relatively rigid molecules. OLEDs incorporating such salts for emitting layers exhibit better thermal stability compared with devices made from the corresponding neutral dyes (H2N-D-NH2). These results suggest that ionic salts may more generally enable the formation of thermally stable, amorphous emitting, and charge transporting layers.

  13. Improving the light-emitting properties of single-layered polyfluorene light-emitting devices by simple ionic liquid blending

    NASA Astrophysics Data System (ADS)

    Horike, Shohei; Nagaki, Hiroto; Misaki, Masahiro; Koshiba, Yasuko; Morimoto, Masahiro; Fukushima, Tatsuya; Ishida, Kenji

    2018-03-01

    This paper describes an evaluation of ionic liquids (ILs) as potential electrolytes for single-layered light-emitting devices with good emission performance. As optoelectronic devices continue to grow in abundance, high-performance light-emitting devices with a single emission layer are becoming increasingly important for low-cost production. We show that a simple technique of osmosing IL into the polymer layer can result in high luminous efficiency and good response times of single-layered light-emitting polymers, even without the additional stacking of charge carrier injection and transport layers. The IL contributions to the light-emission of the polymer are discussed from the perspectives of energy diagrams and of the electric double layers on the electrodes. Our findings enable a faster, cheaper, and lower-in-waste production of light-emitting devices.

  14. A gamma-ray burst at a redshift of z approximately 8.2.

    PubMed

    Tanvir, N R; Fox, D B; Levan, A J; Berger, E; Wiersema, K; Fynbo, J P U; Cucchiara, A; Krühler, T; Gehrels, N; Bloom, J S; Greiner, J; Evans, P A; Rol, E; Olivares, F; Hjorth, J; Jakobsson, P; Farihi, J; Willingale, R; Starling, R L C; Cenko, S B; Perley, D; Maund, J R; Duke, J; Wijers, R A M J; Adamson, A J; Allan, A; Bremer, M N; Burrows, D N; Castro-Tirado, A J; Cavanagh, B; de Ugarte Postigo, A; Dopita, M A; Fatkhullin, T A; Fruchter, A S; Foley, R J; Gorosabel, J; Kennea, J; Kerr, T; Klose, S; Krimm, H A; Komarova, V N; Kulkarni, S R; Moskvitin, A S; Mundell, C G; Naylor, T; Page, K; Penprase, B E; Perri, M; Podsiadlowski, P; Roth, K; Rutledge, R E; Sakamoto, T; Schady, P; Schmidt, B P; Soderberg, A M; Sollerman, J; Stephens, A W; Stratta, G; Ukwatta, T N; Watson, D; Westra, E; Wold, T; Wolf, C

    2009-10-29

    Long-duration gamma-ray bursts (GRBs) are thought to result from the explosions of certain massive stars, and some are bright enough that they should be observable out to redshifts of z > 20 using current technology. Hitherto, the highest redshift measured for any object was z = 6.96, for a Lyman-alpha emitting galaxy. Here we report that GRB 090423 lies at a redshift of z approximately 8.2, implying that massive stars were being produced and dying as GRBs approximately 630 Myr after the Big Bang. The burst also pinpoints the location of its host galaxy.

  15. Flow-aggregated traffic-driven label mapping in label-switching networks

    NASA Astrophysics Data System (ADS)

    Nagami, Kenichi; Katsube, Yasuhiro; Esaki, Hiroshi; Nakamura, Osamu

    1998-12-01

    Label switching technology enables high performance, flexible, layer-3 packet forwarding based on the fixed length label information mapped to the layer-3 packet stream. A Label Switching Router (LSR) forwards layer-3 packets based on their label information mapped to the layer-3 address information as well as their layer-3 address information. This paper evaluates the required number of labels under traffic-driven label mapping policy using the real backbone traffic traces. The evaluation shows that the label mapping policy requires a large number of labels. In order to reduce the required number of labels, we propose a label mapping policy which is a traffic-driven label mapping for the traffic toward the same destination network. The evaluation shows that the proposed label mapping policy requires only about one tenth as many labels compared with the traffic-driven label mapping for the host-pair packet stream,and the topology-driven label mapping for the destination network packet stream.

  16. Nutrition Label Viewing during a Food-Selection Task: Front-of-Package Labels vs Nutrition Facts Labels.

    PubMed

    Graham, Dan J; Heidrick, Charles; Hodgin, Katie

    2015-10-01

    Earlier research has identified consumer characteristics associated with viewing Nutrition Facts labels; however, little is known about those who view front-of-package nutrition labels. Front-of-package nutrition labels might appeal to more consumers than do Nutrition Facts labels, but it might be necessary to provide consumers with information about how to locate and use these labels. This study quantifies Nutrition Facts and front-of-package nutrition label viewing among American adult consumers. Attention to nutrition information was measured during a food-selection task. One hundred and twenty-three parents (mean age=38 years, mean body mass index [calculated as kg/m(2)]=28) and one of their children (aged 6 to 9 years) selected six foods from a university laboratory-turned-grocery aisle. Participants were randomized to conditions in which front-of-package nutrition labels were present or absent, and signage explaining front-of-package nutrition labels was present or absent. Adults' visual attention to Nutrition Facts labels and front-of-package nutrition labels was objectively measured via eye-tracking glasses. To examine whether there were significant differences in the percentages of participants who viewed Nutrition Facts labels vs front-of-package nutrition labels, McNemar's tests were conducted across all participants, as well as within various sociodemographic categories. To determine whether hypothesized factors, such as health literacy and education, had stronger relationships with front-of-package nutrition label vs Nutrition Facts label viewing, linear regression assessed the magnitude of relationships between theoretically and empirically derived factors and each type of label viewing. Overall, front-of-package nutrition labels were more likely to be viewed than Nutrition Facts labels; however, for all subgroups, higher rates of front-of-package nutrition label viewership occurred only when signage was present drawing attention to the presence and

  17. Organic light emitting devices for illumination

    DOEpatents

    Hack, Michael; Lu, Min-Hao Michael; Weaver, Michael S.

    2010-02-16

    An organic light emitting device is provided. The device has a plurality of regions, each region having an organic emissive layer adapted to emit a different spectrum of light. The regions in combination emit light suitable for illumination purposes. The area of each region may be selected such that the device is more efficient that an otherwise equivalent device having regions of equal size. The regions may have an aspect ratio of at least about four. All parts of any given region may be driven at the same current.

  18. Alteration of sensitivity of intratumor quiescent and total cells to gamma-rays following thermal neutron irradiation with or without 10B-compound.

    PubMed

    Masunaga, S; Ono, K; Suzuki, M; Sakurai, Y; Kobayashi, T; Takagaki, M; Kinashi, Y; Akaboshi, M

    2000-02-01

    Changes in the sensitivity of intratumor quiescent (Q) and total cells to gamma-rays following thermal neutron irradiation with or without 10B-compound were examined. 5-Bromo-2'-deoxyuridine (BrdU) was injected to SCC VII tumor-bearing mice intraperitoneally 10 times to label all the proliferating (P) tumor cells. As priming irradiation, thermal neutrons alone or thermal neutrons with 10B-labeled sodium borocaptate (BSH) or dl-p-boronophenylalanine (BPA) were administered. The tumor-bearing mice then received a series of gamma-ray radiation doses, 0 through 24 h after the priming irradiation. During this period, no BrdU was administered. Immediately after the second irradiation, the tumors were excised, minced, and trypsinized. Following incubation of tumor cells with cytokinesis blocker, the micronucleus (MN) frequency in cells without BrdU labeling (= Q cells at the time of priming irradiation) was determined using immunofluorescence staining for BrdU. The MN frequency in the total (P + Q) tumor cells was determined from the tumors that were not pretreated with BrdU before the priming irradiation. To determine the BrdU-labeled cell ratios in the tumors at the time of the second irradiation, each group also included mice that were continuously administered BrdU until just before the second irradiation using mini-osmotic pumps which had been implanted subcutaneously 5 days before the priming irradiation. In total cells, during the interval between the two irradiations, the tumor sensitivity to gamma-rays relative to that immediately after priming irradiation decreased with the priming irradiation ranking in the following order: thermal neutrons only > thermal neutrons with BSH > thermal neutrons with BPA. In contrast, in Q cells, during that time the sensitivity increased in the following order: thermal neutrons only < thermal neutrons with BSH < thermal neutrons with BPA. The longer the interval between the two irradiations, the higher was the BrdU-labeled cell

  19. Unidentified Gamma-Ray Sources: Hunting Gamma-Ray Blazars

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Massaro, F.; D'Abrusco, R.; Tosti, G.

    2012-04-02

    One of the main scientific objectives of the ongoing Fermi mission is unveiling the nature of the unidentified {gamma}-ray sources (UGSs). Despite the large improvements of Fermi in the localization of {gamma}-ray sources with respect to the past {gamma}-ray missions, about one third of the Fermi-detected objects are still not associated to low energy counterparts. Recently, using the Wide-field Infrared Survey Explorer (WISE) survey, we discovered that blazars, the rarest class of Active Galactic Nuclei and the largest population of {gamma}-ray sources, can be recognized and separated from other extragalactic sources on the basis of their infrared (IR) colors. Basedmore » on this result, we designed an association method for the {gamma}-ray sources to recognize if there is a blazar candidate within the positional uncertainty region of a generic {gamma}-ray source. With this new IR diagnostic tool, we searched for {gamma}-ray blazar candidates associated to the UGS sample of the second Fermi {gamma}-ray catalog (2FGL). We found that our method associates at least one {gamma}-ray blazar candidate as a counterpart each of 156 out of 313 UGSs analyzed. These new low-energy candidates have the same IR properties as the blazars associated to {gamma}-ray sources in the 2FGL catalog.« less

  20. UNIDENTIFIED {gamma}-RAY SOURCES: HUNTING {gamma}-RAY BLAZARS

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Massaro, F.; Ajello, M.; D'Abrusco, R.

    2012-06-10

    One of the main scientific objectives of the ongoing Fermi mission is unveiling the nature of unidentified {gamma}-ray sources (UGSs). Despite the major improvements of Fermi in the localization of {gamma}-ray sources with respect to the past {gamma}-ray missions, about one-third of the Fermi-detected objects are still not associated with low-energy counterparts. Recently, using the Wide-field Infrared Survey Explorer survey, we discovered that blazars, the rarest class of active galactic nuclei and the largest population of {gamma}-ray sources, can be recognized and separated from other extragalactic sources on the basis of their infrared (IR) colors. Based on this result, wemore » designed an association method for the {gamma}-ray sources to recognize if there is a blazar candidate within the positional uncertainty region of a generic {gamma}-ray source. With this new IR diagnostic tool, we searched for {gamma}-ray blazar candidates associated with the UGS sample of the second Fermi {gamma}-ray LAT catalog (2FGL). We found that our method associates at least one {gamma}-ray blazar candidate as a counterpart to each of 156 out of 313 UGSs analyzed. These new low-energy candidates have the same IR properties as the blazars associated with {gamma}-ray sources in the 2FGL catalog.« less

  1. Co-Labeling for Multi-View Weakly Labeled Learning.

    PubMed

    Xu, Xinxing; Li, Wen; Xu, Dong; Tsang, Ivor W

    2016-06-01

    It is often expensive and time consuming to collect labeled training samples in many real-world applications. To reduce human effort on annotating training samples, many machine learning techniques (e.g., semi-supervised learning (SSL), multi-instance learning (MIL), etc.) have been studied to exploit weakly labeled training samples. Meanwhile, when the training data is represented with multiple types of features, many multi-view learning methods have shown that classifiers trained on different views can help each other to better utilize the unlabeled training samples for the SSL task. In this paper, we study a new learning problem called multi-view weakly labeled learning, in which we aim to develop a unified approach to learn robust classifiers by effectively utilizing different types of weakly labeled multi-view data from a broad range of tasks including SSL, MIL and relative outlier detection (ROD). We propose an effective approach called co-labeling to solve the multi-view weakly labeled learning problem. Specifically, we model the learning problem on each view as a weakly labeled learning problem, which aims to learn an optimal classifier from a set of pseudo-label vectors generated by using the classifiers trained from other views. Unlike traditional co-training approaches using a single pseudo-label vector for training each classifier, our co-labeling approach explores different strategies to utilize the predictions from different views, biases and iterations for generating the pseudo-label vectors, making our approach more robust for real-world applications. Moreover, to further improve the weakly labeled learning on each view, we also exploit the inherent group structure in the pseudo-label vectors generated from different strategies, which leads to a new multi-layer multiple kernel learning problem. Promising results for text-based image retrieval on the NUS-WIDE dataset as well as news classification and text categorization on several real-world multi

  2. Enhanced Analysis Techniques for an Imaging Neutron and Gamma Ray Spectrometer

    NASA Astrophysics Data System (ADS)

    Madden, Amanda C.

    The presence of gamma rays and neutrons is a strong indicator of the presence of Special Nuclear Material (SNM). The imaging Neutron and gamma ray SPECTrometer (NSPECT) developed by the University of New Hampshire and Michigan Aerospace corporation detects the fast neutrons and prompt gamma rays from fissile material, and the gamma rays from radioactive material. The instrument operates as a double scatter device, requiring a neutron or a gamma ray to interact twice in the instrument. While this detection requirement decreases the efficiency of the instrument, it offers superior background rejection and the ability to measure the energy and momentum of the incident particle. These measurements create energy spectra and images of the emitting source for source identification and localization. The dual species instrument provides superior detection than a single species alone. In realistic detection scenarios, few particles are detected from a potential threat due to source shielding, detection at a distance, high background, and weak sources. This contributes to a small signal to noise ratio, and threat detection becomes difficult. To address these difficulties, several enhanced data analysis tools were developed. A Receiver Operating Characteristic Curve (ROC) helps set instrumental alarm thresholds as well as to identify the presence of a source. Analysis of a dual-species ROC curve provides superior detection capabilities. Bayesian analysis helps to detect and identify the presence of a source through model comparisons, and helps create a background corrected count spectra for enhanced spectroscopy. Development of an instrument response using simulations and numerical analyses will help perform spectra and image deconvolution. This thesis will outline the principles of operation of the NSPECT instrument using the double scatter technology, traditional analysis techniques, and enhanced analysis techniques as applied to data from the NSPECT instrument, and an

  3. Light emitting device having peripheral emissive region

    DOEpatents

    Forrest, Stephen R

    2013-05-28

    Light emitting devices are provided that include one or more OLEDs disposed only on a peripheral region of the substrate. An OLED may be disposed only on a peripheral region of a substantially transparent substrate and configured to emit light into the substrate. Another surface of the substrate may be roughened or include other features to outcouple light from the substrate. The edges of the substrate may be beveled and/or reflective. The area of the OLED(s) may be relatively small compared to the substrate surface area through which light is emitted from the device. One or more OLEDs also or alternatively may be disposed on an edge of the substrate about perpendicular to the surface of the substrate through which light is emitted, such that they emit light into the substrate. A mode expanding region may be included between each such OLED and the substrate.

  4. Flexible bottom-emitting white organic light-emitting diodes with semitransparent Ni/Ag/Ni anode.

    PubMed

    Koo, Ja-Ryong; Lee, Seok Jae; Lee, Ho Won; Lee, Dong Hyung; Yang, Hyung Jin; Kim, Woo Young; Kim, Young Kwan

    2013-05-06

    We fabricated a flexible bottom-emitting white organic light-emitting diode (BEWOLED) with a structure of PET/Ni/Ag/Ni (3/6/3 nm)/ NPB (50 nm)/mCP (10 nm)/7% FIrpic:mCP (10 nm)/3% Ir(pq)(2) acac:TPBi (5 nm)/7% FIrpic:TPBi (5 nm)/TPBi (10 nm)/Liq (2 nm)/ Al (100 nm). To improve the performance of the BEWOLED, a multilayered metal stack anode of Ni/Ag/Ni treated with oxygen plasma for 60 sec was introduced into the OLED devices. The Ni/Ag/Ni anode effectively enhanced the probability of hole-electron recombination due to an efficient hole injection into and charge balance in an emitting layer. By comparing with a reference WOLED using ITO on glass, it is verified that the flexible BEWOLED showed a similar or better electroluminescence (EL) performance.

  5. Single-view 3D reconstruction of correlated gamma-neutron sources

    DOE PAGES

    Monterial, Mateusz; Marleau, Peter; Pozzi, Sara A.

    2017-01-05

    We describe a new method of 3D image reconstruction of neutron sources that emit correlated gammas (e.g. Cf- 252, Am-Be). This category includes a vast majority of neutron sources important in nuclear threat search, safeguards and non-proliferation. Rather than requiring multiple views of the source this technique relies on the source’s intrinsic property of coincidence gamma and neutron emission. As a result only a single-view measurement of the source is required to perform the 3D reconstruction. In principle, any scatter camera sensitive to gammas and neutrons with adequate timing and interaction location resolution can perform this reconstruction. Using a neutronmore » double scatter technique, we can calculate a conical surface of possible source locations. By including the time to a correlated gamma we further constrain the source location in three-dimensions by solving for the source-to-detector distance along the surface of said cone. As a proof of concept we applied these reconstruction techniques on measurements taken with the the Mobile Imager of Neutrons for Emergency Responders (MINER). Two Cf-252 sources measured at 50 and 60 cm from the center of the detector were resolved in their varying depth with average radial distance relative resolution of 26%. To demonstrate the technique’s potential with an optimized system we simulated the measurement in MCNPX-PoliMi assuming timing resolution of 200 ps (from 2 ns in the current system) and source interaction location resolution of 5 mm (from 3 cm). Furthermore, these simulated improvements in scatter camera performance resulted in radial distance relative resolution decreasing to an average of 11%.« less

  6. Single-view 3D reconstruction of correlated gamma-neutron sources

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Monterial, Mateusz; Marleau, Peter; Pozzi, Sara A.

    We describe a new method of 3D image reconstruction of neutron sources that emit correlated gammas (e.g. Cf- 252, Am-Be). This category includes a vast majority of neutron sources important in nuclear threat search, safeguards and non-proliferation. Rather than requiring multiple views of the source this technique relies on the source’s intrinsic property of coincidence gamma and neutron emission. As a result only a single-view measurement of the source is required to perform the 3D reconstruction. In principle, any scatter camera sensitive to gammas and neutrons with adequate timing and interaction location resolution can perform this reconstruction. Using a neutronmore » double scatter technique, we can calculate a conical surface of possible source locations. By including the time to a correlated gamma we further constrain the source location in three-dimensions by solving for the source-to-detector distance along the surface of said cone. As a proof of concept we applied these reconstruction techniques on measurements taken with the the Mobile Imager of Neutrons for Emergency Responders (MINER). Two Cf-252 sources measured at 50 and 60 cm from the center of the detector were resolved in their varying depth with average radial distance relative resolution of 26%. To demonstrate the technique’s potential with an optimized system we simulated the measurement in MCNPX-PoliMi assuming timing resolution of 200 ps (from 2 ns in the current system) and source interaction location resolution of 5 mm (from 3 cm). Furthermore, these simulated improvements in scatter camera performance resulted in radial distance relative resolution decreasing to an average of 11%.« less

  7. Photoaffinity labelling of the cardiac calcium channel. (-)-[3H]azidopine labels a 165 kDa polypeptide, and evidence against a [3H]-1,4-dihydropyridine-isothiocyanate being a calcium-channel-specific affinity ligand.

    PubMed

    Ferry, D R; Goll, A; Glossmann, H

    1987-04-01

    The arylazide 1,4-dihydropyridine (-)-[3H]azidopine binds to a saturable population of sites in guinea-pig heart membranes with a dissociation constant (KD) of 30 +/- 7 pM and a density (Bmax.) of 670 +/- 97 fmol/mg of protein. This high-affinity binding site is assumed to reside on voltage-operated calcium channels because reversible binding is blocked stereoselectively by 1,4-dihydropyridine channel blockers and by the enantiomers of Bay K 8644. A low-affinity (KD 25 +/- 7 nM) high-capacity (Bmax. 21.6 +/- 9 pmol/mg of protein) site does not bind (-)- or (+)-Bay K 8644, but is blocked by high concentrations (greater than 500 nM) of dihydro-2,6-dimethyl-4-(2-isothiocyanatophenyl)-3,5-pyridinedicarboxy lic acid dimethyl ester (1,4-DHP-isothiocyanate) or, e.g., (+/-)-nicardipine. (-)-[3H]Azidopine was photoincorporated covalently into bands of 165 +/- 8, 39 +/- 2 and 35 +/- 3 kDa, as determined by SDS/polyacrylamide-gel electrophoresis. Labelling of the 165 kDa band is protected stereoselectively by 1,4-dihydropyridine enantiomers at low (nM) concentrations and by (-)- and (+)-Bay K 8644, whereas the lower-Mr bands are not. Thus, only the 165 kDa band is the calcium-channel-linked 1,4-dihydropyridine receptor. Photolabelling of the 39 or 35 kDa bands was only blocked by 10 microM-1,4-DHP-isothiocyanate or 50 microM-(+/-)-nicardipine but not by 10 microM-(-)-Bay K 8644. [3H]-1,4-DHP-isothiocyanate binds to guinea-pig heart membranes with a KD of 0.35 nM and dissociates with a k-1 of 0.2 min-1 at 30 degrees C. [3H]-1,4 DHP-isothiocyanate irreversibly labels bands of 39 and 35 kDa which are protected by greater than 10 microM-(+/-)-nicardipine or unlabelled ligand but not by 10 microM-(-)-Bay K 8644. Thus, [3H]-1,4-DHP-isothiocyanate is not an affinity probe for the calcium channel.

  8. Infrared emitting device and method

    DOEpatents

    Kurtz, S.R.; Biefeld, R.M.; Dawson, L.R.; Howard, A.J.; Baucom, K.C.

    1997-04-29

    The infrared emitting device comprises a III-V compound semiconductor substrate upon which are grown a quantum-well active region having a plurality of quantum-well layers formed of a ternary alloy comprising InAsSb sandwiched between barrier layers formed of a ternary alloy having a smaller lattice constant and a larger energy bandgap than the quantum-well layers. The quantum-well layers are preferably compressively strained to increase the threshold energy for Auger recombination; and a method is provided for determining the preferred thickness for the quantum-well layers. Embodiments of the present invention are described having at least one cladding layer to increase the optical and carrier confinement in the active region, and to provide for waveguiding of the light generated within the active region. Examples have been set forth showing embodiments of the present invention as surface- and edge-emitting light emitting diodes (LEDs), an optically-pumped semiconductor laser, and an electrically-injected semiconductor diode laser. The light emission from each of the infrared emitting devices of the present invention is in the midwave infrared region of the spectrum from about 2 to 6 microns. 8 figs.

  9. VLBI OBSERVATIONS OF THE JET IN M 87 DURING THE VERY HIGH ENERGY {gamma}-RAY FLARE IN 2010 APRIL

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hada, Kazuhiro; Giroletti, Marcello; Giovannini, Gabriele

    2012-11-20

    We report on the detailed radio status of the M 87 jet during the very high energy (VHE) {gamma}-ray flaring event in 2010 April, obtained from high-resolution, multi-frequency, phase-referencing Very Long Baseline Array observations. We especially focus on the properties of the jet base (the radio core) and the peculiar knot HST-1, which are currently favored as the {gamma}-ray emitting sites. During the VHE flaring event, the HST-1 region remains stable in terms of its structure and flux density in the optically thin regime above 2 GHz, being consistent with no signs of enhanced activities reported at X-ray for thismore » feature. The radio core shows an inverted spectrum at least up to 43 GHz during this event. Astrometry of the core position, which is specified as {approx}20 R {sub s} from the central engine in our previous study, shows that the core position is stable on a level of 4 R {sub s}. The core at 43 and 22 GHz tends to show slightly ({approx}10%) higher flux level near the date of the VHE flux peak compared with the epochs before/after the event. The size of the 43 GHz core is estimated to be {approx}17 R {sub s}, which is close to the size of the emitting region suggested from the observed timescale of rapid variability at VHE. These results tend to favor the scenario that the VHE {gamma}-ray flare in 2010 April is associated with the radio core.« less

  10. Influences of wide-angle and multi-beam interference on the chromaticity and efficiency of top-emitting white organic light-emitting diodes

    NASA Astrophysics Data System (ADS)

    Deng, Lingling; Zhou, Hongwei; Chen, Shufen; Shi, Hongying; Liu, Bin; Wang, Lianhui; Huang, Wei

    2015-02-01

    Wide-angle interference (WI) and multi-beam interference (MI) in microcavity are analyzed separately to improve chromaticity and efficiency of the top-emitting white organic light-emitting diodes (TWOLEDs). A classic electromagnetic theory is used to calculate the resonance intensities of WI and MI in top-emitting organic light-emitting diodes (TOLEDs) with influence factors (e.g., electrodes and exciton locations) being considered. The role of WI on the performances of TOLEDs is revealed through using δ-doping technology and comparing blue and red EML positions in top-emitting and bottom-emitting devices. The blue light intensity significantly increases and the chromaticity of TWOLEDs is further improved with the use of enhanced WI (the blue emitting layer moving towards the reflective electrode) in the case of a weak MI. In addition, the effect of the thicknesses of light output layer and carrier transport layers on WI and MI are also investigated. Apart from the microcavity effect, other factors, e.g., carrier balance and carrier recombination regions are considered to obtain TWOLEDs with high efficiency and improved chromaticity near white light equal-energy point.

  11. Using triple gamma coincidences with a pixelated semiconductor Compton-PET scanner: a simulation study

    NASA Astrophysics Data System (ADS)

    Kolstein, M.; Chmeissani, M.

    2016-01-01

    The Voxel Imaging PET (VIP) Pathfinder project presents a novel design using pixelated semiconductor detectors for nuclear medicine applications to achieve the intrinsic image quality limits set by physics. The conceptual design can be extended to a Compton gamma camera. The use of a pixelated CdTe detector with voxel sizes of 1 × 1 × 2 mm3 guarantees optimal energy and spatial resolution. However, the limited time resolution of semiconductor detectors makes it impossible to use Time Of Flight (TOF) with VIP PET. TOF is used in order to improve the signal to noise ratio (SNR) by using only the most probable portion of the Line-Of-Response (LOR) instead of its entire length. To overcome the limitation of CdTe time resolution, we present in this article a simulation study using β+-γ emitting isotopes with a Compton-PET scanner. When the β+ annihilates with an electron it produces two gammas which produce a LOR in the PET scanner, while the additional gamma, when scattered in the scatter detector, provides a Compton cone that intersects with the aforementioned LOR. The intersection indicates, within a few mm of uncertainty along the LOR, the origin of the beta-gamma decay. Hence, one can limit the part of the LOR used by the image reconstruction algorithm.

  12. An upper limit on interstellar C IV in the spectrum of gamma-2 Velorum

    NASA Technical Reports Server (NTRS)

    Lengyel-Frey, D.; Stecher, T. P.; West, D. K.

    1975-01-01

    An upper limit on the column density of C IV along the line of sight to gamma-2 Vel is derived from upper limits placed on the equivalent widths of the interstellar C IV doublet with rest wavelengths at 1548.20 A and 1550.77 A. A lower limit of 250,000 K is calculated for the electron temperature of O VI emitting regions by combining the C IV results with a measurement of the column density of interstellar O VI for the same star and using calculations for the relative ionization of some abundant elements as a function of electron temperature in a low-density plasma. Since gamma-2 Vel is in the central part of the Gum Nebula, the high temperature suggested by these results is shown to support the idea that a high-temperature phase of the interstellar medium, possibly maintained by supernova explosions, may exist.-

  13. Prompt Gamma Imaging for In Vivo Range Verification of Pencil Beam Scanning Proton Therapy.

    PubMed

    Xie, Yunhe; Bentefour, El Hassane; Janssens, Guillaume; Smeets, Julien; Vander Stappen, François; Hotoiu, Lucian; Yin, Lingshu; Dolney, Derek; Avery, Stephen; O'Grady, Fionnbarr; Prieels, Damien; McDonough, James; Solberg, Timothy D; Lustig, Robert A; Lin, Alexander; Teo, Boon-Keng K

    2017-09-01

    To report the first clinical results and value assessment of prompt gamma imaging for in vivo proton range verification in pencil beam scanning mode. A stand-alone, trolley-mounted, prototype prompt gamma camera utilizing a knife-edge slit collimator design was used to record the prompt gamma signal emitted along the proton tracks during delivery of proton therapy for a brain cancer patient. The recorded prompt gamma depth detection profiles of individual pencil beam spots were compared with the expected profiles simulated from the treatment plan. In 6 treatment fractions recorded over 3 weeks, the mean (± standard deviation) range shifts aggregated over all spots in 9 energy layers were -0.8 ± 1.3 mm for the lateral field, 1.7 ± 0.7 mm for the right-superior-oblique field, and -0.4 ± 0.9 mm for the vertex field. This study demonstrates the feasibility and illustrates the distinctive benefits of prompt gamma imaging in pencil beam scanning treatment mode. Accuracy in range verification was found in this first clinical case to be better than the range uncertainty margin applied in the treatment plan. These first results lay the foundation for additional work toward tighter integration of the system for in vivo proton range verification and quantification of range uncertainties. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Evidence for solar flare directivity from the Gamma-Ray Spectrometer aboard the SMM satellite

    NASA Technical Reports Server (NTRS)

    Vestrand, W. T.; Forrest, D. J.; Chupp, E. L.; Rieger, E.; Share, G. H.

    1986-01-01

    A number of observations from the SMM Gamma-Ray Spectrometer are presented that altogether strongly indicate that the high-energy emission from flares is anisotropic. They are: (1) the fraction of events detected at energies above 300 keV near the limb is significantly higher than is expected for isotropically emitting flares; (2) there is a statistically significant center-to-limb variation in the 300-1000-keV spectra of flares; and (3) nearly all of the events detected at above 10 MeV are located near the limb.

  15. A Search for Early Optical Emission at Gamma-Ray Burst Locations by the Solar Mass Ejection Imager (SMEI)

    NASA Technical Reports Server (NTRS)

    Band, David L.; Buffington, Andrew; Jackson, Bernard V.; Hick, P. Paul; Smith, Aaron C.

    2005-01-01

    The Solar Mass Ejection Imager (SMEI) views nearly every point on the sky once every 102 minutes and can detect point sources as faint as R approx. 10th magnitude. Therefore, SMEI can detect or provide upper limits for the optical afterglow from gamma-ray bursts in the tens of minutes after the burst when different shocked regions may emit optically. Here we provide upper limits for 58 bursts between 2003 February and 2005 April.

  16. Light emitting fabric technologies for photodynamic therapy.

    PubMed

    Mordon, Serge; Cochrane, Cédric; Tylcz, Jean Baptiste; Betrouni, Nacim; Mortier, Laurent; Koncar, Vladan

    2015-03-01

    Photodynamic therapy (PDT) is considered to be a promising method for treating various types of cancer. A homogeneous and reproducible illumination during clinical PDT plays a determinant role in preventing under- or over-treatment. The development of flexible light sources would considerably improve the homogeneity of light delivery. The integration of optical fiber into flexible structures could offer an interesting alternative. This paper aims to describe different methods proposed to develop Side Emitting Optical Fibers (SEOF), and how these SEOF can be integrated in a flexible structure to improve light illumination of the skin during PDT. Four main techniques can be described: (i) light blanket integrating side-glowing optical fibers, (ii) light emitting panel composed of SEOF obtained by micro-perforations of the cladding, (iii) embroidery-based light emitting fabric, and (iv) woven-based light emitting fabric. Woven-based light emitting fabrics give the best performances: higher fluence rate, best homogeneity of light delivery, good flexibility. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Growth kinetics of gamma-prime precipitates in a directionally solidified eutectic, gamma/gamma-prime-delta

    NASA Technical Reports Server (NTRS)

    Tewari, S. N.

    1976-01-01

    A directionally solidified eutectic alloy (DSEA), of those viewed as potential candidates for the next generation of aircraft gas turbine blade materials, is studied for the gamma-prime growth kinetics, in the system Ni-Nb-Cr-Al, specifically: Ni-20 w/o Nb-6 w/o Cr-2.5 w/o Al gamma/gamma-prime-delta DSEA. Heat treatment, polishing and etching, and preparation for electron micrography are described, and the size distribution of gamma-prime phase following various anneals is plotted, along with gamma-prime growth kinetics in this specific DSEA, and the cube of gamma-prime particle size vs anneal time. Activation energies and coarsening kinetics are studied.

  18. The neutron-gamma Feynman variance to mean approach: Gamma detection and total neutron-gamma detection (theory and practice)

    NASA Astrophysics Data System (ADS)

    Chernikova, Dina; Axell, Kåre; Avdic, Senada; Pázsit, Imre; Nordlund, Anders; Allard, Stefan

    2015-05-01

    Two versions of the neutron-gamma variance to mean (Feynman-alpha method or Feynman-Y function) formula for either gamma detection only or total neutron-gamma detection, respectively, are derived and compared in this paper. The new formulas have particular importance for detectors of either gamma photons or detectors sensitive to both neutron and gamma radiation. If applied to a plastic or liquid scintillation detector, the total neutron-gamma detection Feynman-Y expression corresponds to a situation where no discrimination is made between neutrons and gamma particles. The gamma variance to mean formulas are useful when a detector of only gamma radiation is used or when working with a combined neutron-gamma detector at high count rates. The theoretical derivation is based on the Chapman-Kolmogorov equation with the inclusion of general reactions and corresponding intensities for neutrons and gammas, but with the inclusion of prompt reactions only. A one energy group approximation is considered. The comparison of the two different theories is made by using reaction intensities obtained in MCNPX simulations with a simplified geometry for two scintillation detectors and a 252Cf-source. In addition, the variance to mean ratios, neutron, gamma and total neutron-gamma are evaluated experimentally for a weak 252Cf neutron-gamma source, a 137Cs random gamma source and a 22Na correlated gamma source. Due to the focus being on the possibility of using neutron-gamma variance to mean theories for both reactor and safeguards applications, we limited the present study to the general analytical expressions for Feynman-alpha formulas.

  19. Organic light emitting devices for illumination

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hack, Michael; Lu, Min-Hao Michael; Weaver, Michael S

    An organic light emitting device an a method of obtaining illumination from such a device is provided. The device has a plurality of regions, each region having an organic emissive layer adapted to emit a different spectrum of light. The regions in combination emit light suitable for illumination purposes. The area of each region may be selected such that the device is more efficient than an otherwise equivalent device having regions of equal size. The regions may have an aspect ratio of at least about four. All parts of any given region may be driven at the same current.

  20. Side-emitting fiber optic position sensor

    DOEpatents

    Weiss, Jonathan D [Albuquerque, NM

    2008-02-12

    A side-emitting fiber optic position sensor and method of determining an unknown position of an object by using the sensor. In one embodiment, a concentrated beam of light source illuminates the side of a side-emitting fiber optic at an unknown axial position along the fiber's length. Some of this side-illuminated light is in-scattered into the fiber and captured. As the captured light is guided down the fiber, its intensity decreases due to loss from side-emission away from the fiber and from bulk absorption within the fiber. By measuring the intensity of light emitted from one (or both) ends of the fiber with a photodetector(s), the axial position of the light source is determined by comparing the photodetector's signal to a calibrated response curve, look-up table, or by using a mathematical model. Alternatively, the side-emitting fiber is illuminated at one end, while a photodetector measures the intensity of light emitted from the side of the fiber, at an unknown position. As the photodetector moves further away from the illuminated end, the detector's signal strength decreases due to loss from side-emission and/or bulk absorption. As before, the detector's signal is correlated to a unique position along the fiber.

  1. Perovskite Materials for Light-Emitting Diodes and Lasers.

    PubMed

    Veldhuis, Sjoerd A; Boix, Pablo P; Yantara, Natalia; Li, Mingjie; Sum, Tze Chien; Mathews, Nripan; Mhaisalkar, Subodh G

    2016-08-01

    Organic-inorganic hybrid perovskites have cemented their position as an exceptional class of optoelectronic materials thanks to record photovoltaic efficiencies of 22.1%, as well as promising demonstrations of light-emitting diodes, lasers, and light-emitting transistors. Perovskite materials with photoluminescence quantum yields close to 100% and perovskite light-emitting diodes with external quantum efficiencies of 8% and current efficiencies of 43 cd A(-1) have been achieved. Although perovskite light-emitting devices are yet to become industrially relevant, in merely two years these devices have achieved the brightness and efficiencies that organic light-emitting diodes accomplished in two decades. Further advances will rely decisively on the multitude of compositional, structural variants that enable the formation of lower-dimensionality layered and three-dimensional perovskites, nanostructures, charge-transport materials, and device processing with architectural innovations. Here, the rapid advancements in perovskite light-emitting devices and lasers are reviewed. The key challenges in materials development, device fabrication, operational stability are addressed, and an outlook is presented that will address market viability of perovskite light-emitting devices. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  2. Recent advances in conjugated polymers for light emitting devices.

    PubMed

    Alsalhi, Mohamad Saleh; Alam, Javed; Dass, Lawrence Arockiasamy; Raja, Mohan

    2011-01-01

    A recent advance in the field of light emitting polymers has been the discovery of electroluminescent conjugated polymers, that is, kind of fluorescent polymers that emit light when excited by the flow of an electric current. These new generation fluorescent materials may now challenge the domination by inorganic semiconductor materials of the commercial market in light-emitting devices such as light-emitting diodes (LED) and polymer laser devices. This review provides information on unique properties of conjugated polymers and how they have been optimized to generate these properties. The review is organized in three sections focusing on the major advances in light emitting materials, recent literature survey and understanding the desirable properties as well as modern solid state lighting and displays. Recently, developed conjugated polymers are also functioning as roll-up displays for computers and mobile phones, flexible solar panels for power portable equipment as well as organic light emitting diodes in displays, in which television screens, luminous traffic, information signs, and light-emitting wallpaper in homes are also expected to broaden the use of conjugated polymers as light emitting polymers. The purpose of this review paper is to examine conjugated polymers in light emitting diodes (LEDs) in addition to organic solid state laser. Furthermore, since conjugated polymers have been approved as light-emitting organic materials similar to inorganic semiconductors, it is clear to motivate these organic light-emitting devices (OLEDs) and organic lasers for modern lighting in terms of energy saving ability. In addition, future aspects of conjugated polymers in LEDs were also highlighted in this review.

  3. Recent Advances in Conjugated Polymers for Light Emitting Devices

    PubMed Central

    AlSalhi, Mohamad Saleh; Alam, Javed; Dass, Lawrence Arockiasamy; Raja, Mohan

    2011-01-01

    A recent advance in the field of light emitting polymers has been the discovery of electroluminescent conjugated polymers, that is, kind of fluorescent polymers that emit light when excited by the flow of an electric current. These new generation fluorescent materials may now challenge the domination by inorganic semiconductor materials of the commercial market in light-emitting devices such as light-emitting diodes (LED) and polymer laser devices. This review provides information on unique properties of conjugated polymers and how they have been optimized to generate these properties. The review is organized in three sections focusing on the major advances in light emitting materials, recent literature survey and understanding the desirable properties as well as modern solid state lighting and displays. Recently, developed conjugated polymers are also functioning as roll-up displays for computers and mobile phones, flexible solar panels for power portable equipment as well as organic light emitting diodes in displays, in which television screens, luminous traffic, information signs, and light-emitting wallpaper in homes are also expected to broaden the use of conjugated polymers as light emitting polymers. The purpose of this review paper is to examine conjugated polymers in light emitting diodes (LEDs) in addition to organic solid state laser. Furthermore, since conjugated polymers have been approved as light-emitting organic materials similar to inorganic semiconductors, it is clear to motivate these organic light-emitting devices (OLEDs) and organic lasers for modern lighting in terms of energy saving ability. In addition, future aspects of conjugated polymers in LEDs were also highlighted in this review. PMID:21673938

  4. Fluorescently labelled multiplex lateral flow immunoassay based on cadmium-free quantum dots.

    PubMed

    Beloglazova, Natalia V; Sobolev, Aleksander M; Tessier, Mickael D; Hens, Zeger; Goryacheva, Irina Yu; De Saeger, Sarah

    2017-03-01

    A sensitive tool for simultaneous qualitative detection of two mycotoxins based on use of non-cadmium quantum dots (QDs) is presented for the first time. QDs have proven themselves as promising fluorescent labels for biolabeling and chemical analysis. With an increasing global tendency to regulate and limit the use of hazardous elements, indium phosphide (InP) QDs are highlighted as environmentally-friendly alternatives to the highly efficient and well-studied, but potentially toxic Cd- and Pb-based QDs. Here, we developed water-soluble InP QDs-based fluorescent nanostructures. They consisted of core/shell InP/ZnS QDs enrobed in a silica shell that allowed the water solubility (QD@SiO 2 ). Then we applied the QD@SiO 2 as novel, silica shell-encapsulated fluorescent labels in immunoassays for rapid multiplexed screening. Two mycotoxins, zearalenone and deoxynivalenol, were simultaneously detected in maize and wheat, since the two QD@SiO 2 labelled conjugates emit at two different, individually detectable wavelengths. The cutoff values for the simultaneous determination were 50 and 500μgkg -1 for zearalenone and deoxynivalenol, respectively, in both maize and wheat. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the result. Copyright © 2017 Elsevier Inc. All rights reserved.

  5. Green emitting phosphors and blends thereof

    DOEpatents

    Setlur, Anant Achyut; Siclovan, Oltea Puica; Nammalwar, Prasanth Kumar; Sathyanarayan, Ramesh Rao; Porob, Digamber G.; Chandran, Ramachandran Gopi; Heward, William Jordan; Radkov, Emil Vergilov; Briel, Linda Jane Valyou

    2010-12-28

    Phosphor compositions, blends thereof and light emitting devices including white light emitting LED based devices, and backlights, based on such phosphor compositions. The devices include a light source and a phosphor material as described. Also disclosed are phosphor blends including such a phosphor and devices made therefrom.

  6. Label Review Training: Module 1: Label Basics, Page 21

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about types of labels.

  7. High-Z Nanoparticle/Polymer Nanocomposites for Gamma-Ray Scintillation Detectors

    NASA Astrophysics Data System (ADS)

    Liu, Chao

    -Z inorganic nanoparticles. A facile single-precursor method is first developed to synthesize HfO2 nanoparticles, the highest-Z simple oxide with band gap larger than polyvinyltoluene, with uniform size distribution around 5 nm. A nanoparticle-surface-modification protocol is then developed for the fabrication of transparent nanocomposite monoliths with high nanoparticle loadings (up to 40 wt%). Using this method, transparent HfO2-loaded blue-emitting nanocomposite scintillators (2 mm thick, transmittance at 550 nm >75%) have been fabricated capable of producing a full energy photopeak for 662 keV gamma rays, with the best deconvoluted photopeak energy resolution < 8%, representing a significant improvement over previous nanoparticle-based nanocomposite scintillators. Although the HfO2 work represents a great improvement over previous reports, it is also found in this system that the light yield deteriorates at higher nanoparticle loadings. This is attributed to the trapping of fast electron energy deposited in the non-emitting nanoparticles. To overcome this deterioration issue, a revisit to the previously proposed quantum-dot-loaded nanocomposite scintillator is made with significant improvements. Transparent, ultra-high-loading (up to 60 wt%) CdxZn1-xS/ZnS core/shell quantum dot/polymer nanocomposite monoliths are first synthesized by in situ copolymerization of the partially mathacrylate-functionalized quantum dots in a monomer solution. With efficient Forster resonance energy transfer from the high-atomic-number quantum dots to lower-band-gap organic dyes, quantum-dot-borne excitons are extracted for photon production. The resulting nanocomposites thus exhibit unprecedented simultaneous enhancements in both light yield (visible photons produced per MeV of gamma photon energy) and gamma attenuation power. In a best demonstration, a 60 wt% quantum-dot nanocomposite scintillator exhibits a light yield of 9255 photons/MeV and a photopeak resolution of 9.8% under 662 keV Cs

  8. Label Review Training: Module 1: Label Basics, Page 20

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. This section focuses on supplemental labeling.

  9. Label Review Training: Module 1: Label Basics, Page 22

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about what labels require review.

  10. Label Review Training: Module 1: Label Basics, Page 19

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. This section covers supplemental distributor labeling.

  11. Rapid diagnosis of occult abscesses using sup 99m Tc-labeled monoclonal antibodies

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Coons, T.A.; Rhodes, B.A.; Thakur, M.L.

    1991-01-01

    Acute infections, such as appendicitis and occult infections in AIDS patients, can be diagnosed within two hours by gamma scintigraphy after i.v. administration of {sup 99m}Tc labeled antibodies reactive with human granulocytes. The antibody, murine IgM anti-SSEA-1, is partially reduced using Sn(II) to expose and protect reactive sulfide groups. The antibody is then purified, stannous tartrate and stabilizers are added, and the mixture is lyophilized. To label, sodium pertechnetate is added. After a 15 minute incubation the tracer drug is injected. The rate of accumulation and degree of concentration at the site of infection is presumptively determinative of the severitymore » of the infection. Acceptance criteria and tests for the {sup 99m}Tc labeled antibody product have been established and validated. Greater than 93% of the {sup 99m}Tc is firmly bound to the protein as determined by quantitative HPLC. Radiochemical impurities, colloidal {sup 99m}Tc and free pertechnetate are together less than 4% as determined by thin layer chromatography. The immunoreactive fraction, measured by binding to solid phase antigen, and affinity measured be ELISA, are unchanged by the {sup 99m}Tc-direct labeling process. Two hour blood clearance in rats is within 90% of the value of the {sup 125}I labeled analog. The immunoreactive fraction decreases less than 10% when incubated in human plasma for 24 hours. This method has been compared to other direct labeling methods, and found to give higher radiochemical yields. 5 figs.« less

  12. Influences of wide-angle and multi-beam interference on the chromaticity and efficiency of top-emitting white organic light-emitting diodes

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Deng, Lingling; Zhou, Hongwei; Chen, Shufen, E-mail: iamsfchen@njupt.edu.cn

    Wide-angle interference (WI) and multi-beam interference (MI) in microcavity are analyzed separately to improve chromaticity and efficiency of the top-emitting white organic light-emitting diodes (TWOLEDs). A classic electromagnetic theory is used to calculate the resonance intensities of WI and MI in top-emitting organic light-emitting diodes (TOLEDs) with influence factors (e.g., electrodes and exciton locations) being considered. The role of WI on the performances of TOLEDs is revealed through using δ-doping technology and comparing blue and red EML positions in top-emitting and bottom-emitting devices. The blue light intensity significantly increases and the chromaticity of TWOLEDs is further improved with the usemore » of enhanced WI (the blue emitting layer moving towards the reflective electrode) in the case of a weak MI. In addition, the effect of the thicknesses of light output layer and carrier transport layers on WI and MI are also investigated. Apart from the microcavity effect, other factors, e.g., carrier balance and carrier recombination regions are considered to obtain TWOLEDs with high efficiency and improved chromaticity near white light equal-energy point.« less

  13. Reshaping Light-Emitting Diodes To Increase External Efficiency

    NASA Technical Reports Server (NTRS)

    Rogowski, Robert; Egalon, Claudio

    1995-01-01

    Light-emitting diodes (LEDs) reshaped, according to proposal, increasing amount of light emitted by decreasing fraction of light trapped via total internal reflection. Results in greater luminous output power for same electrical input power; greater external efficiency. Furthermore, light emitted by reshaped LEDs more nearly collimated (less diffuse). Concept potentially advantageous for conventional red-emitting LEDs. More advantageous for new "blue" LEDs, because luminous outputs and efficiencies of these devices very low. Another advantage, proposed conical shapes achieved relatively easily by chemical etching of semiconductor surfaces.

  14. Label Review Training: Module 1: Label Basics, Page 18

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. This section discusses the types of labels.

  15. Label Review Training: Module 1: Label Basics, Page 26

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about mandatory and advisory label statements.

  16. Label Review Training: Module 1: Label Basics, Page 15

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about the consequences of improper labeling.

  17. Label Review Training: Module 1: Label Basics, Page 14

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about positive effects from proper labeling.

  18. Label Review Training: Module 1: Label Basics, Page 24

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. This page is about which labels require review.

  19. Label Review Training: Module 1: Label Basics, Page 17

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. See an overview of the importance of labels.

  20. Label Review Training: Module 1: Label Basics, Page 27

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. See examples of mandatory and advisory label statements.

  1. Triazine herbicide resistance in the photosynthetic bacterium Rhodopseudomonas sphaeroides

    PubMed Central

    Brown, Alfred E.; Gilbert, Carl W.; Guy, Rachel; Arntzen, Charles J.

    1984-01-01

    The photoaffinity herbicide azidoatrazine (2-azido-4-ethylamino-6-isopropylamino-s-triazine) selectively labels the L subunit of the reaction center of the photosynthetic bacterium Rhodopseudomonas sphaeroides. Herbicide-resistant mutants retain the L subunit and have altered binding properties for methylthio- and chloro-substituted triazines as well as altered equilibrium constants for electron transfer between primary and secondary electron acceptors. We suggest that a subtle alteration in the L subunit is responsible for herbicide resistance and that the L subunit is the functional analog of the 32-kDa QB protein of chloroplast membranes. Images PMID:16593520

  2. Label Review Training: Module 1: Label Basics, Page 23

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Lists types of labels that do not require review.

  3. Is bone mineral density measurement using dual-energy X-ray absorptiometry affected by gamma rays?

    PubMed

    Xie, Liang-Jun; Li, Jian-Fang; Zeng, Feng-Wei; Jiang, Hang; Cheng, Mu-Hua; Chen, Yi

    2013-01-01

    The objective of this study was to determine whether the gamma rays emitted from the radionuclide effect bone mineral density (BMD) measurement. Nine subjects (mean age: 56 ± 17.96 yr) scheduled for bone scanning underwent BMD measurement using dual-energy X-ray absorptiometry (DXA) (Hologic/Discovery A) before and 1, 2, and 4 h after injection of technetium-99m-methylene diphosphonate (99mTc-MDP). Ten subjects (mean age: 41 ± 15.47 yr) scheduled for therapy of differentiated thyroid carcinoma with iodine-131 underwent BMD measurement before and 2 h after therapeutic radionuclide administration. All patients were given whole body BMD measurement, including head, arm, ribs, lumbar spine, pelvis, and leg sites. Besides, patients who referred to radioiodine therapy were given total hip and femoral neck BMD measurement as well. No statistically significant changes in BMD values were detected after 99mTc-MDP and iodine-131 administration for all measurement sites (p > 0.05), and individual difference of BMD before and after radionuclide imaging or therapy was less than the least significant change in lumbar spine, total hip, and femoral neck. In conclusion, BMD measurements are not influenced by the gamma rays emitted from technetium-99m and iodine-131. DXA bone densitometry may be performed simultaneously with bone scanning and radioiodine therapy. Copyright © 2013 The International Society for Clinical Densitometry. Published by Elsevier Inc. All rights reserved.

  4. A photo-cleavable biotin affinity tag for the facile release of a photo-crosslinked carbohydrate-binding protein.

    PubMed

    Chang, Tsung-Che; Adak, Avijit K; Lin, Ting-Wei; Li, Pei-Jhen; Chen, Yi-Ju; Lai, Chain-Hui; Liang, Chien-Fu; Chen, Yu-Ju; Lin, Chun-Cheng

    2016-03-15

    The use of photo-crosslinking glycoprobes represents a powerful strategy for the covalent capture of labile protein complexes and allows detailed characterization of carbohydrate-mediated interactions. The selective release of target proteins from solid support is a key step in functional proteomics. We envisaged that light activation can be exploited for releasing labeled protein in a dual photo-affinity probe-based strategy. To investigate this possibility, we designed a trifunctional, galactose-based, multivalent glycoprobe for affinity labeling of carbohydrate-binding proteins. The resulting covalent protein-probe adduct is attached to a photo-cleavable biotin affinity tag; the biotin moiety enables specific presentation of the conjugate on streptavidin-coated beads, and the photolabile linker allows the release of the labeled proteins. This dual probe promotes both the labeling and the facile cleavage of the target protein complexes from the solid surfaces and the remainder of the cell lysate in a completely unaltered form, thus eliminating many of the common pitfalls associated with traditional affinity-based purification methods. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Mathematical Investigation of Gamma Ray and Neutron Absorption Grid Patterns for Homeland Defense Related Fourier Imaging Systems

    NASA Technical Reports Server (NTRS)

    Boccio, Dona

    2003-01-01

    Terrorist suitcase nuclear devices typically using converted Soviet tactical nuclear warheads contain several kilograms of plutonium. This quantity of plutonium emits a significant number of gamma rays and neutrons as it undergoes radioactive decay. These gamma rays and neutrons normally penetrate ordinary matter to a significant distance. Unfortunately this penetrating quality of the radiation makes imaging with classical optics impractical. However, this radiation signature emitted by the nuclear source may be sufficient to be imaged from low-flying aerial platforms carrying Fourier imaging systems. The Fourier imaging system uses a pair of co-aligned absorption grids to measure a selected range of spatial frequencies from an object. These grids typically measure the spatial frequency in only one direction at a time. A grid pair that looks in all directions simultaneously would be an improvement over existing technology. A number of grid pairs governed by various parameters were investigated to solve this problem. By examining numerous configurations, it became apparent that an appropriate spiral pattern could be made to work. A set of equations was found to describe a grid pattern that produces straight fringes. Straight fringes represent a Fourier transform of a point source at infinity. An inverse Fourier transform of this fringe pattern would provide an accurate image (location and intensity) of a point source.

  6. Fluorescent nanocolloids for differential labeling of the endocytic pathway and drug delivery applications

    NASA Astrophysics Data System (ADS)

    Delehanty, James B.; Spillmann, Christopher M.; Naciri, Jawad; Algar, W. Russ; Ratna, Banahalli R.; Medintz, Igor L.

    2013-02-01

    The demonstration of fine control over nanomaterials within biological systems, particularly in live cells, is integral for the successful implementation of nanoparticles (NPs) in biomedical applications. Here, we show the ability to differentially label the endocytic pathway of mammalian cells in a spatiotemporal manner utilizing fluorescent nanocolloids (NCs) doped with a perylene-based dye. EDC-based conjugation of green- and red-emitting NCs to the iron transport protein transferrin resulted in stable bioconjugates that were efficiently endocytosed by HEK 293T/17 cells. The staggered delivery of the bioconjugates allowed for the time-resolved, differential labeling of distinct vesicular compartments along the endocytic pathway in a nontoxic manner. We further demonstrated the ability of the NCs to be impregnated with the anticancer therapeutic, doxorubicin. Delivery of the drug-doped nanoconjugates resulted in the intracellular release and nuclear accumulation of doxorubicin in a time- and dose-dependent manner. We discuss our results in the context of the utility of such materials for NP-mediated drug delivery applications.

  7. Label Review Training: Module 1: Label Basics, Page 16

    EPA Pesticide Factsheets

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about the importance of labels and the role in enforcement.

  8. Epoxyethylglycyl peptides as inhibitors of oligosaccharyltransferase: double-labelling of the active site.

    PubMed

    Bause, E; Wesemann, M; Bartoschek, A; Breuer, W

    1997-02-15

    Pig liver oligosaccharyltransferase (OST) is inactivated irreversibly by a hexapeptide in which threonine has been substituted by epoxyethylglycine in the Asn-Xaa-Thr glycosylation triplet. Incubation of the enzyme in the presence of Dol-PP-linked [14C]oligosaccharides and the N-3,5-dinitrobenzoylated epoxy derivative leads to the double-labelling of two subunits (48 and 66 kDa) of the oligomeric OST complex, both of which are involved in the catalytic activity. Labelling of both subunits was blocked competitively by the acceptor peptide N-benzoyl-Asu-Gly-Thr-NHCH3 and by the OST inhibitor N-benzoyl-alpha,gamma-diaminobutyric acid-Gly-Thr-NHCH3, but not by an analogue derived from the epoxy-inhibitor by replacing asparagine with glutamine. Our data clearly show that double-labelling is an active-site-directed modification, involving inhibitor glycosylation at asparagine and covalent attachment of the glycosylated inhibitor, via the epoxy group, to the enzyme. Double-labelling of OST can occur as the result of either a consecutive or a syn-catalytic reaction sequence. The latter mechanism, during the course of which OST catalyses its own 'suicide' inactivation, is more likely, as suggested by indirect experimental evidence. The syn-catalytic mechanism corresponds with our current view of the functional role of the acceptor site Thr/Ser acting as a hydrogen-bond acceptor, not a donor, during transglycosylation.

  9. Wheat Under LED's (Light Emitting Diodes)

    NASA Technical Reports Server (NTRS)

    2004-01-01

    Astroculture is a suite of technologies used to produce and maintain a closed controlled environment for plant growth. The two most recent missions supported growth of potato, dwarf wheat, and mustard plants, and provided scientists with the first opportunity to conduct true plant research in space. Light emitting diodes have particular usefulness for plant growth lighting because they emit a much smaller amount of radiant heat than do conventional lighting sources and because they have potential of directing a higher percentage of the emitted light onto plants surfaces. Furthermore, the high output LED's have emissions in the 600-700 nm waveband, which is of highest efficiency for photosynthesis by plants.

  10. An exceptionally bright flare from SGR 1806-20 and the origins of short-duration gamma-ray bursts.

    PubMed

    Hurley, K; Boggs, S E; Smith, D M; Duncan, R C; Lin, R; Zoglauer, A; Krucker, S; Hurford, G; Hudson, H; Wigger, C; Hajdas, W; Thompson, C; Mitrofanov, I; Sanin, A; Boynton, W; Fellows, C; von Kienlin, A; Lichti, G; Rau, A; Cline, T

    2005-04-28

    Soft-gamma-ray repeaters (SGRs) are galactic X-ray stars that emit numerous short-duration (about 0.1 s) bursts of hard X-rays during sporadic active periods. They are thought to be magnetars: strongly magnetized neutron stars with emissions powered by the dissipation of magnetic energy. Here we report the detection of a long (380 s) giant flare from SGR 1806-20, which was much more luminous than any previous transient event observed in our Galaxy. (In the first 0.2 s, the flare released as much energy as the Sun radiates in a quarter of a million years.) Its power can be explained by a catastrophic instability involving global crust failure and magnetic reconnection on a magnetar, with possible large-scale untwisting of magnetic field lines outside the star. From a great distance this event would appear to be a short-duration, hard-spectrum cosmic gamma-ray burst. At least a significant fraction of the mysterious short-duration gamma-ray bursts may therefore come from extragalactic magnetars.

  11. Very high-energy gamma rays from gamma-ray bursts.

    PubMed

    Chadwick, Paula M

    2007-05-15

    Very high-energy (VHE) gamma-ray astronomy has undergone a transformation in the last few years, with telescopes of unprecedented sensitivity having greatly expanded the source catalogue. Such progress makes the detection of a gamma-ray burst at the highest energies much more likely than previously. This paper describes the facilities currently operating and their chances for detecting gamma-ray bursts, and reviews predictions for VHE gamma-ray emission from gamma-ray bursts. Results to date are summarized.

  12. Gamma-ray mirror technology for NDA of spent fuel

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Descalle, M. A.; Ruz-Armendariz, J.; Decker, T.

    Direct measurements of gamma rays emitted by fissile material have been proposed as an alternative to measurements of the gamma rays from fission products. From a safeguards applications perspective, direct detection of uranium (U) and plutonium (Pu) K-shell fluorescence emission lines and specific lines from some of their isotopes could lead to improved shipper-receiver difference or input accountability at the start of Pu reprocessing. However, these measurements are difficult to implement when the spent fuel is in the line-of-sight of the detector, as the detector is exposed to high rates dominated by fission product emissions. To overcome the combination ofmore » high rates and high background, grazing incidence multilayer mirrors have been proposed as a solution to selectively reflect U and Pu hard X-ray and soft gamma rays in the 90 to 420 keV energy into a high-purity germanium (HPGe) detector shielded from the direct line-of-sight of spent fuel. Several groups demonstrated that K-shell fluorescence lines of U and Pu in spent fuel could be detected with Ge detectors. In the field of hard X-ray optics the performance of reflective multilayer coated reflective optics was demonstrated up to 645 keV at the European Synchrotron Radiation Facility. Initial measurements conducted at Oak Ridge National Laboratory with sealed sources and scoping experiments conducted at the ORNL Irradiated Fuels Examination Laboratory (IFEL) with spent nuclear fuel further demonstrated the pass-band properties of multilayer mirrors for reflecting specific emission lines into 1D and 2D HPGe detectors, respectively.« less

  13. Broadband mid-infrared superlattice light-emitting diodes

    NASA Astrophysics Data System (ADS)

    Ricker, R. J.; Provence, S. R.; Norton, D. T.; Boggess, T. F.; Prineas, J. P.

    2017-05-01

    InAs/GaSb type-II superlattice light-emitting diodes were fabricated to form a device that provides emission over the entire 3-5 μm mid-infrared transmission window. Variable bandgap emission regions were coupled together using tunnel junctions to emit at peak wavelengths of 3.3 μm, 3.5 μm, 3.7 μm, 3.9 μm, 4.1 μm, 4.4 μm, 4.7 μm, and 5.0 μm. Cascading the structure recycles the electrons in each emission region to emit several wavelengths simultaneously. At high current densities, the light-emitting diode spectra broadened into a continuous, broadband spectrum that covered the entire mid-infrared band. When cooled to 77 K, radiances of over 1 W/cm2 sr were achieved, demonstrating apparent temperatures above 1000 K over the 3-5 μm band. InAs/GaSb type-II superlattices are capable of emitting from 3 μm to 30 μm, and the device design can be expanded to include longer emission wavelengths.

  14. Modelling gamma-ray light curves of phase-aligned millisecond pulsars

    NASA Astrophysics Data System (ADS)

    Chang, Shan; Zhang, Li; Li, Xiang; Jiang, Zejun

    2018-04-01

    Three gamma-ray millisecond pulsars (MSPs), PSR J1939+2134, PSR J1959+2048, and PSR J0034-0534, have been confirmed to have a common feature of phase-aligned in radio and gamma-ray bands. With a geometric (two-pole caustic) model and a physical outer gap model (revised 3D outer gap model) in a three dimensional (3D) retarded magnetic dipole with a perturbation magnetic field, the observed features of these MSPs are studied. In order to obtained the best-fitting model parameters, the Markov chain Monte Carlo technique is used and reasonable GeV band light curves for three MSPs are given. Our calculations indicate that MSPs emit high energy photons with smaller inclination angles (α ≈ 10°-50°), larger view angles (ζ ≈ 65°-100°), and smaller perturbation factor (ɛ ≈ -0.15-0.1). Note that the factor ɛ, describing the strength of the perturbed magnetic field, is all less than zero in these two models, so the magnetic field caused by current-induced play a leading role in the pulsed location of MSPs.

  15. Observations of gamma-ray pulsars at the highest energies with the Fermi Large Area Telescope

    NASA Astrophysics Data System (ADS)

    Saz Parkinson, Pablo

    2016-07-01

    One of the most exciting developments in pulsar astrophysics in recent years has been the detection, with ground-based instruments (VERITAS, MAGIC), of pulsed gamma-ray emission from the Crab at very high energies (VHE, E>100 GeV). The Large Area Telescope (LAT) on board the Fermi satellite has detected over 160 pulsars above 100 MeV. Twenty-eight of these have been shown to emit pulsations above 10 GeV and approximately a dozen show emission above 25 GeV. While most gamma-ray pulsars are well-fitted in the GeV range by a power law with an exponential cut-off at around a few GeV, some emission models predict emission at energies above 100 GeV, either through a power-law extrapolation of the low-energy spectrum, or via a new (e.g. Inverse Compton) component. We will present results of our search for high-energy emission from LAT-detected gamma-ray pulsars using the latest Pass 8 data and discuss the prospects of finding the next VHE pulsar, providing a good target (or targets) for follow-up observations with current and future ground-based observatories, like CTA.

  16. Effect of gamma radiation on growth and survival of common seed-borne fungi in India

    NASA Astrophysics Data System (ADS)

    Maity, J. P.; Chakraborty, A.; Chanda, S.; Santra, S. C.

    2008-07-01

    The present work describes radiation-induced effects of major seeds like Oryza sativa Cv-2233, Oryza sativa Cv-Shankar, Cicer arietinum Cv-local and seed-borne fungi like Alternaria sp., Aspergillus sp., Trichoderma sp. and Curvularia sp. 60Co gamma source at 25 °C emitting gamma ray at 1173 and 1332 keV energy was used for irradiation. Dose of gamma irradiation up to 3 kGy (0.12 kGy/h) was applied for exposing the seed and fungal spores. Significant depletion of the fungal population was noted with irradiation at 1-2 kGy, whereas germinating potential of the treated grain did not alter significantly. However, significant differential radiation response in delayed seed germination, colony formation of the fungal spores and their depletion of growth were noticed in a dose-dependent manner. The depletion of the fungal viability (germination) was noted within the irradiation dose range of 1-2 kGy for Alternaria sp. and Aspergillus sp., while 0.5-1 kGy for Trichoderma sp. and Curvularia sp. However, complete inhibition of all the selected fungi was observed above 2.5 kGy.

  17. Long Persistent Light Emitting Diode Indicators

    ERIC Educational Resources Information Center

    Jia, Dongdong; Ma, Yiwei; Hunter, D. N.

    2007-01-01

    An undergraduate laboratory was designed for undergraduate students to make long persistent light emitting diode (LED) indicators using phosphors. Blue LEDs, which emit at 465 nm, were characterized and used as an excitation source. Long persistent phosphors, SrAl[subscript 2]O[subscript 4]:Eu[superscript 2+],Dy[superscript 3+] (green) and…

  18. Synthesis of carbon-14 and stable isotope labeled Avagacestat: a novel gamma secretase inhibitor for the treatment of Alzheimer's disease.

    PubMed

    Burrell, Richard C; Easter, John A; Cassidy, Michael P; Gillman, Kevin W; Olson, Richard E; Bonacorsi, Samuel J

    2014-08-01

    Bristol-Myers Squibb and others are developing drugs that target novel mechanisms to combat Alzheimer's disease. γ-Secretase inhibitors are one class of potential therapies that have received considerable attention. (R)-2-(4-Chloro-N-(2-fluoro-4-(1,2,4-oxadiazol-3-yl)benzyl)phenylsulfonamido)-5,5,5-trifluoropentanamide (Avagacestat) is a γ-secretase-inhibiting drug that has been investigated by Bristol-Myers Squibb in preclinical and clinical studies. An important step in the development process was the synthesis of a carbon-14-labeled analog for use in a human absorption, distribution, metabolism, and excretion study and a stable isotope labeled analog for use as a standard in bioanalytical assays to accurately quantify the concentration of the drug in biological samples. Carbon-14 labeled Avagacestat was synthesized in seven steps in a 33% overall yield from carbon-14 labeled potassium cyanide. A total of 5.95 mCi was prepared with a specific activity of 0.81 μCi/mg and a radiochemical purity of 99.9%. (13) C6 -Labeled Avagacestat was synthesized in three steps in a 15% overall yield from 4-chloro[(13) C6 ]aniline. A total of 585 mg was prepared with a ultraviolet purity of 99.9%. Copyright © 2014 John Wiley & Sons, Ltd.

  19. Applying the new gamma ray imager diagnostic to measurements of runaway electron Bremsstrahlung radiation in the DIII-D Tokamak (invited)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Cooper, C. M., E-mail: coopercm@fusion.gat.com; Pace, D. C.; Paz-Soldan, C.

    2016-11-15

    A new gamma ray imager (GRI) is developed to probe the electron distribution function with 2D spatial resolution during runaway electron (RE) experiments at the DIII-D tokamak. The diagnostic is sensitive to 0.5–100 MeV gamma rays, allowing characterization of the RE distribution function evolution during RE growth and dissipation. The GRI consists of a lead “pinhole camera” mounted on the DIII-D midplane with 123 honeycombed tangential chords 20 cm wide that span the vessel interior. Up to 30 bismuth germanate (BGO) scintillation detectors capture RE bremsstrahlung radiation for Pulse Height Analysis (PHA) capable of discriminating up to 20 000 pulses permore » second. Digital signal processing routines combining shaping filters are performed during PHA to reject noise and record gamma ray energy. The GRI setup and PHA algorithms will be described and initial data from experiments will be presented. A synthetic diagnostic is developed to generate the gamma ray spectrum of a GRI channel given the plasma information and a prescribed distribution function. Magnetic reconstructions of the plasma are used to calculate the angle between every GRI sightline and orient and discriminate gamma rays emitted by a field-aligned RE distribution function.« less

  20. Applying the new gamma ray imager diagnostic to measurements of runaway electron Bremsstrahlung radiation in the DIII-D Tokamak (invited)

    DOE PAGES

    Cooper, C. M.; Pace, D. C.; Paz-Soldan, C.; ...

    2016-08-30

    A new gamma ray imager (GRI) is developed to probe the electron distribution function with 2D spatial resolution during runaway electron (RE) experiments at the DIII-D tokamak. The diagnostic is sensitive to 0.5-100 MeV gamma rays, allowing characterization of the RE distribution function evolution during RE growth and dissipation. The GRI consists of a lead "pinhole camera" mounted on the DIII-D midplane with 123 honeycombed tangential chords 20 cm wide that span the vessel interior. Up to 30 bismuth germanate (BGO) scintillation detectors capture RE bremsstrahlung radiation for Pulse Height Analysis (PHA) capable of discriminating up to 20,000 pulses permore » second. Digital signal processing routines combining shaping filters are performed during PHA to reject noise and record gamma ray energy. The GRI setup and PHA algorithms will be described and initial data from experiments will be presented. A synthetic diagnostic is developed to generate the gamma ray spectrum of a GRI channel given the plasma information and a prescribed distribution function. Furthermore, magnetic reconstructions of the plasma are used to calculate the angle between every GRI sightline and orient and discriminate gamma rays emitted by a field-aligned RE distribution function.« less

  1. TIME STRUCTURE OF GAMMA-RAY SIGNALS GENERATED IN LINE-OF-SIGHT INTERACTIONS OF COSMIC RAYS FROM DISTANT BLAZARS

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Prosekin, Anton; Aharonian, Felix; Essey, Warren

    2012-10-01

    Blazars are expected to produce both gamma rays and cosmic rays. Therefore, observed high-energy gamma rays from distant blazars may contain a significant contribution from secondary gamma rays produced along the line of sight by the interactions of cosmic-ray protons with background photons. Unlike the standard models of blazars that consider only the primary photons emitted at the source, models that include the cosmic-ray contribution predict that even {approx}10 TeV photons should be detectable from distant objects with redshifts as high as z {>=} 0.1. Secondary photons contribute to signals of point sources only if the intergalactic magnetic fields aremore » very small, B {approx}< 10{sup -14} G, and their detection can be used to set upper bounds on magnetic fields along the line of sight. Secondary gamma rays have distinct spectral and temporal features. We explore the temporal properties of such signals using a semi-analytical formalism and detailed numerical simulations, which account for all the relevant processes, including magnetic deflections. In particular, we elucidate the interplay of time delays coming from the proton deflections and from the electromagnetic cascade, and we find that, at multi-TeV energies, secondary gamma rays can show variability on timescales of years for B {approx} 10{sup -15} G.« less

  2. Measuring the labeling efficiency of pseudocontinuous arterial spin labeling.

    PubMed

    Chen, Zhensen; Zhang, Xingxing; Yuan, Chun; Zhao, Xihai; van Osch, Matthias J P

    2017-05-01

    Optimization and validation of a sequence for measuring the labeling efficiency of pseudocontinuous arterial spin labeling (pCASL) perfusion MRI. The proposed sequence consists of a labeling module and a single slice Look-Locker echo planar imaging readout. A model-based algorithm was used to calculate labeling efficiency from the signal acquired from the main brain-feeding arteries. Stability of the labeling efficiency measurement was evaluated with regard to the use of cardiac triggering, flow compensation and vein signal suppression. Accuracy of the measurement was assessed by comparing the measured labeling efficiency to mean brain pCASL signal intensity over a wide range of flip angles as applied in the pCASL labeling. Simulations show that the proposed algorithm can effectively calculate labeling efficiency when correcting for T1 relaxation of the blood spins. Use of cardiac triggering and vein signal suppression improved stability of the labeling efficiency measurement, while flow compensation resulted in little improvement. The measured labeling efficiency was found to be linearly (R = 0.973; P < 0.001) related to brain pCASL signal intensity over a wide range of pCASL flip angles. The optimized labeling efficiency sequence provides robust artery-specific labeling efficiency measurement within a short acquisition time (∼30 s), thereby enabling improved accuracy of pCASL CBF quantification. Magn Reson Med 77:1841-1852, 2017. © 2016 International Society for Magnetic Resonance in Medicine Magn Reson Med 77:1841-1852, 2017. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

  3. Gamma-spectrometric measurement of radioactivity in agricultural soils of the Lombardia region, northern Italy.

    PubMed

    Guidotti, Laura; Carini, Franca; Rossi, Riccardo; Gatti, Marina; Cenci, Roberto M; Beone, Gian Maria

    2015-04-01

    This work is part of a wider monitoring project of the agricultural soils in Lombardia, which aims to build a database of topsoil properties and the potentially toxic elements, organic pollutants and gamma emitting radionuclides that the topsoils contain. A total of 156 agricultural soils were sampled according to the LUCAS (Land Use/Cover Area frame statistical Survey) standard procedure. The aim was to provide a baseline to document the conditions present at the time of sampling. The results of the project concerning soil radioactivity are presented here. The aim was to assess the content of (238)U, (232)Th, (137)Cs and (40)K by measuring soil samples by gamma spectrometry. (238)U, (232)Th and (40)K activities range 24-231, 20-70, and 242-1434 Bq kg(-1) respectively. The geographic distribution of (238)U reflects the geophysical framework of the Lombardia region: the soils with high content of uranium are distributed for the most part in the South Alpine belt, where the presence of magmatic rocks is widespread. These soils show an higher activity of (238)U than of (232)Th. The (238)U activities become lower than (232)Th when soils are located in the plain, originating from basic sedimentary rocks. (137)Cs activity ranges 0.4-86.8 kBq m(-2). The lowest activity of (137)Cs is in the plain, whereas the highest is in the North on soils kept as lawn or pasture. The (137)Cs activity of some samples suggests the presence of accumulation processes that lead to (137)Cs enriched soils. This is the first survey of gamma emitting radionuclides in Lombardia that is based on the LUCAS standard sampling. The results from this monitoring campaign are important for the human radiation exposure and provide the zero point, which will be useful for assessing future effects due to external factors such as human activities. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  4. Flash photoionization of gamma-ray burst environments

    NASA Technical Reports Server (NTRS)

    Band, David L.; Hartmann, Dieter H.

    1992-01-01

    The H-alpha line emission that a flash-photoionized region emits is calculated. Archival transients, as well as various theoretical predictions, suggest that there may be significant ionizing flux. The limits on the line flux which might be observable indicate that the density must be fairly high for the recombination radiation to be observable. The intense burst radiation is insufficient to melt the dust which will be present in such a dense medium. This dust may attenuate the observable line emission, but does not attenuate the ionizing radiation before it ionizes the neutral medium surrounding the burst source. The dust can also produce a light echo. If there are indeed gamma-ray bursts in dense clouds, then it is possible that the burst was triggered by Bondi-Hoyle accretion from the dense medium, although it is unlikely on statistical grounds that all bursts occur in clouds.

  5. The gamma-ray emitting region of the jet in Cyg X-3

    NASA Astrophysics Data System (ADS)

    Zdziarski, Andrzej A.; Sikora, Marek; Dubus, Guillaume; Yuan, Feng; Cerutti, Benoit; Ogorzałek, Anna

    2012-04-01

    We study models of the γ-ray emission of Cyg X-3 observed by Fermi. We calculate the average X-ray spectrum during the γ-ray active periods. Then, we calculate spectra from Compton scattering of a photon beam into a given direction by isotropic relativistic electrons with a power-law distribution, both based on the Klein-Nishina cross-section and in the Thomson limit. Applying the results to scattering of stellar blackbody radiation in the inner jet of Cyg X-3, we find that a low-energy break in the electron distribution at a Lorentz factor of ˜300-103 is required by the shape of the observed X-ray/γ-ray spectrum in order to avoid overproducing the observed X-ray flux. The electrons giving rise to the observed γ-rays are efficiently cooled by Compton scattering, and the power-law index of the acceleration process is ≃2.5-3. The bulk Lorentz factor of the jet and the kinetic power before the dissipation region depend on the fraction of the dissipation power supplied to the electrons; if it is ≃1/2, the Lorentz factor is ˜2.5, and the kinetic power is ˜1038 erg s-1, which represents a firm lower limit on the jet power, and is comparable to the bolometric luminosity of Cyg X-3. Most of the power supplied to the electrons is radiated. The broad-band spectrum constrains the synchrotron and self-Compton emission from the γ-ray emitting electrons, which requires the magnetic field to be relatively weak, with the magnetic energy density ≲ a few times 10-3 of that in the electrons. The actual value of the magnetic field strength can be inferred from a future simultaneous measurement of the infrared and γ-ray fluxes.

  6. Calculations of the skyshine gamma-ray dose rates from independent spent fuel storage installations (ISFSI) under worst case accident conditions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pace, J.V. III; Cramer, S.N.; Knight, J.R.

    1980-09-01

    Calculations of the skyshine gamma-ray dose rates from three spent fuel storage pools under worst case accident conditions have been made using the discrete ordinates code DOT-IV and the Monte Carlo code MORSE and have been compared to those of two previous methods. The DNA 37N-21G group cross-section library was utilized in the calculations, together with the Claiborne-Trubey gamma-ray dose factors taken from the same library. Plots of all results are presented. It was found that the dose was a strong function of the iron thickness over the fuel assemblies, the initial angular distribution of the emitted radiation, and themore » photon source near the top of the assemblies. 16 refs., 11 figs., 7 tabs.« less

  7. Particle-Induced Gamma-ray Emission Spectroscopy Over a Broad Range of Elements

    NASA Astrophysics Data System (ADS)

    Olds, Hannah; Wilkinson, John; Tighe, Meghanne; McLallen, Walter; McGuire, Patrick

    2017-09-01

    Ion beam analysis is a common application of nuclear physics that allows elemental and isotopic information about materials to be determined from accelerated light ion beams One of the best know ion beam analysis techniques is Particle-Induced Gamma-ray Emission (PIGE) spectroscopy, which can be used ex vacuo to identify the elements of interest in almost any solid target. The energies of the gamma-rays emitted by excited nuclei will be unique to each element and depend on its nuclear structure. For the most sensitivity, the accelerated ions should exceed the Coulomb barrier of the target, but many isotopes are known to be accessible to PIGE even below the Coulomb barrier. To explore the sensitivity of PIGE across the periodic table, PIGE measurements were made on elements with Z = 5, 9, 11-15, 17, 19-35, 37, 42, 44-48, 53, 56, 60, 62, 73, and 74 using 3.4 MeV protons. These measurements will be compared with literature values and be used as a basis for comparison with higher-energy proton beams available at the University of Notre Dame's St. Andre accelerator when it comes online this Fall. The beam normalization technique of using atmospheric argon and its 1459 keV gamma-ray to better estimate the integrated beam on target will also be discussed. Funded by the NSF REU program and the University of Notre Dame.

  8. Multiwavelength Observations of the Blazar BL Lacertae: A New Fast TeV Gamma-Ray Flare

    NASA Astrophysics Data System (ADS)

    Abeysekara, A. U.; Benbow, W.; Bird, R.; Brantseg, T.; Brose, R.; Buchovecky, M.; Buckley, J. H.; Bugaev, V.; Connolly, M. P.; Cui, W.; Daniel, M. K.; Falcone, A.; Feng, Q.; Finley, J. P.; Fortson, L.; Furniss, A.; Gillanders, G. H.; Gunawardhana, I.; Hütten, M.; Hanna, D.; Hervet, O.; Holder, J.; Hughes, G.; Humensky, T. B.; Johnson, C. A.; Kaaret, P.; Kar, P.; Kertzman, M.; Krennrich, F.; Lang, M. J.; Lin, T. T. Y.; McArthur, S.; Moriarty, P.; Mukherjee, R.; O’Brien, S.; Ong, R. A.; Otte, A. N.; Park, N.; Petrashyk, A.; Pohl, M.; Pueschel, E.; Quinn, J.; Ragan, K.; Reynolds, P. T.; Richards, G. T.; Roache, E.; Rulten, C.; Sadeh, I.; Santander, M.; Sembroski, G. H.; Shahinyan, K.; Wakely, S. P.; Weinstein, A.; Wells, R. M.; Wilcox, P.; Williams, D. A.; Zitzer, B.; The VERITAS Collaboration; Jorstad, S. G.; Marscher, A. P.; Lister, M. L.; Kovalev, Y. Y.; Pushkarev, A. B.; Savolainen, T.; Agudo, I.; Molina, S. N.; Gómez, J. L.; Larionov, V. M.; Borman, G. A.; Mokrushina, A. A.; Tornikoski, M.; Lähteenmäki, A.; Chamani, W.; Enestam, S.; Kiehlmann, S.; Hovatta, T.; Smith, P. S.; Pontrelli, P.

    2018-04-01

    Combined with measurements made by very-long-baseline interferometry, the observations of fast TeV gamma-ray flares probe the structure and emission mechanism of blazar jets. However, only a handful of such flares have been detected to date, and only within the last few years have these flares been observed from lower-frequency-peaked BL Lac objects and flat-spectrum radio quasars. We report on a fast TeV gamma-ray flare from the blazar BL Lacertae observed by the Very Energetic Radiation Imaging Telescope Array System (VERITAS). with a rise time of ∼2.3 hr and a decay time of ∼36 min. The peak flux above 200 GeV is (4.2 ± 0.6) × 10‑6 photon m‑2 s‑1 measured with a 4-minute-binned light curve, corresponding to ∼180% of the flux that is observed from the Crab Nebula above the same energy threshold. Variability contemporaneous with the TeV gamma-ray flare was observed in GeV gamma-ray, X-ray, and optical flux, as well as in optical and radio polarization. Additionally, a possible moving emission feature with superluminal apparent velocity was identified in Very Long Baseline Array observations at 43 GHz, potentially passing the radio core of the jet around the time of the gamma-ray flare. We discuss the constraints on the size, Lorentz factor, and location of the emitting region of the flare, and the interpretations with several theoretical models that invoke relativistic plasma passing stationary shocks.

  9. COSMOLOGY WITH THE Ep,i - Eiso CORRELATION OF GAMMA-RAY BURSTS

    NASA Astrophysics Data System (ADS)

    Amati, Lorenzo

    2012-03-01

    Gamma-Ray Bursts (GRBs) are the brightest sources in the universe, emit mostly in the hard X-ray energy band and have been detected at redshifts up to about 8.2. Thus, they are in principle very powerful probes for cosmology. I shortly review the researches aimed to use GRBs for the measurement of cosmological parameters, which are mainly based on the correlation between spectral peak photon energy and total radiated energy or luminosity. In particular, based on an enriched sample of 120 GRBs, I will provide an update of the analysis by Amati et al. (2008) aimed at extracting information on ΩM and, to a less extent, on ΩΛ, from the Ep,i - Eiso correlation.

  10. Detection of Spectral Evolution in the Bursts Emitted During the 2008-2009 Active Episode of SGR J1550 - 5418

    NASA Technical Reports Server (NTRS)

    von Kienlin, Andreas; Gruber, David; Kouveliotou, Chryssa; Granot, Jonathan; Baring, Matthew G.; Gogus, Ersin; Huppenkothen, Daniela; Kaneko, Yuki; Lin, Lin; Watts, Anna L.; hide

    2012-01-01

    In early October 2008, the Soft Gamma Repeater SGRJ1550 - 5418 (1E1547.0 - 5408, AXJ155052 - 5418, PSR J1550 - 5418) became active, emitting a series of bursts which triggered the Fermi Gamma-ray Burst Monitor (GBM) after which a second especially intense activity period commenced in 2009 January and a third, less active period was detected in 2009 March-April. Here we analyze the GBM data of all the bursts from the first and last active episodes. We performed temporal and spectral analysis for all events and found that their temporal characteristics are very similar to the ones of other SGR bursts, as well the ones reported for the bursts of the main episode (average burst durations 170ms). In addition, we used our sample of bursts to quantify the systematic uncertainties of the GBM location algorithm for soft gamma-ray transients to less than or equal to 8 degrees. Our spectral analysis indicates significant spectral evolution between the first and last set of events. Although the 2008 October events are best fit with a single blackbody function, for the 2009 bursts an Optically Thin Thermal Bremsstrahlung (OTTB) is clearly preferred. We attribute this evolution to changes in the magnetic field topology of the source, possibly due to effects following the very energetic main bursting episode.

  11. Accumulation and dissipation of positive charges induced on a PMMA build-up cap of an ionisation chamber by (60)Co gamma-ray irradiation.

    PubMed

    Morishita, Y; Takata, N

    2013-07-01

    The signal current from an ionisation chamber with a PMMA build-up cap decreases with irradiation time due to electric fields produced by positive charges induced on the cap. In the present study, it was confirmed that the signal current decreases faster for irradiation using narrower (60)Co gamma-ray beams. This is because the number of secondary electrons that are emitted from surrounding materials and penetrate the build-up cap is smaller in a narrower gamma-ray beam, so that fewer positive charges are neutralised. The ionisation chamber was first subjected to continuous gamma-ray irradiation for 24 h, following which it was irradiated with shorter periodic gamma-ray bursts while measuring the current signal. This allowed the coefficients of positive charge accumulation and dissipation to be determined. It was found that the dissipation coefficient has a large constant value during gamma-ray irradiation and decreases asymptotically to a small value after irradiation is stopped. From the coefficients, the minimum signal current was calculated, which is the value when accumulation and dissipation balance each other under continuous irradiation. The time required for the signal current to recover following irradiation was also calculated.

  12. Correlation Analysis of Prompt Emission from Gamma Ray Bursts

    NASA Astrophysics Data System (ADS)

    Pothapragada, Sriharsha

    Prompt emission from gamma-ray bursts (GRBs) exhibits very rapid, complicated temporal and spectral evolution. This diverse variability in the light-curves reflects the complicated nature of the underlying physics, in which inter-penetrating relativistic shells in the outflow are believed to generate strong magnetic fields that vary over very small scales. We use the theory of jitter radiation to model the emission from such regions and the resulting overall prompt gamma ray emission from a series of relativistic collisionless shocks. We present simulated GRB light-curves developed as a series of "pulses" corresponding to instantaneously illuminated "thin-shell" regions emitting via the jitter radiation mechanism. The effects of various geometries, viewing angles, and bulk Lorentz factor profiles of the radiating outflow jets on the spectral features and evolution of these light-curves are explored. Our results demonstrate how an anisotropic jitter radiation pattern, in conjunction with relativistic shock kinematics, can produce certain features observed in the GRB prompt emission spectra, such as the occurrence of hard, synchrotron violating spectra, the "tracking" of observed flux with spectral parameters, and spectral softening below peak energy within individual episodes of the light curve. We highlight predictions in the light of recent advances in the observational sphere of GRBs.

  13. A didactic experiment showing the Compton scattering by means of a clinical gamma camera.

    PubMed

    Amato, Ernesto; Auditore, Lucrezia; Campennì, Alfredo; Minutoli, Fabio; Cucinotta, Mariapaola; Sindoni, Alessandro; Baldari, Sergio

    2017-06-01

    We describe a didactic approach aimed to explain the effect of Compton scattering in nuclear medicine imaging, exploiting the comparison of a didactic experiment with a gamma camera with the outcomes from a Monte Carlo simulation of the same experimental apparatus. We employed a 99m Tc source emitting 140.5keV photons, collimated in the upper direction through two pinholes, shielded by 6mm of lead. An aluminium cylinder was placed on the source at 50mm of distance. The energy of the scattered photons was measured on the spectra acquired by the gamma camera. We observed that the gamma ray energy measured at each step of rotation gradually decreased from the characteristic energy of 140.5keV at 0° to 102.5keV at 120°. A comparison between the obtained data and the expected results from the Compton formula and from the Monte Carlo simulation revealed a full agreement within the experimental error (relative errors between -0.56% and 1.19%), given by the energy resolution of the gamma camera. Also the electron rest mass has been evaluated satisfactorily. The experiment was found useful in explaining nuclear medicine residents the phenomenology of the Compton scattering and its importance in the nuclear medicine imaging, and it can be profitably proposed during the training of medical physics residents as well. Copyright © 2017 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

  14. A Novel Approach in the Weakly Interacting Massive Particle Quest: Cross-correlation of Gamma-Ray Anisotropies and Cosmic Shear

    NASA Astrophysics Data System (ADS)

    Camera, Stefano; Fornasa, Mattia; Fornengo, Nicolao; Regis, Marco

    2013-07-01

    Both cosmic shear and cosmological gamma-ray emission stem from the presence of dark matter (DM) in the universe: DM structures are responsible for the bending of light in the weak-lensing regime and those same objects can emit gamma rays, either because they host astrophysical sources (active galactic nuclei or star-forming galaxies) or directly by DM annihilations (or decays, depending on the properties of the DM particle). Such gamma rays should therefore exhibit strong correlation with the cosmic shear signal. In this Letter, we compute the cross-correlation angular power spectrum of cosmic shear and gamma rays produced by the annihilation/decay of weakly interacting massive particle DM, as well as by astrophysical sources. We show that this observable provides novel information on the composition of the extragalactic gamma-ray background (EGB), since the amplitude and shape of the cross-correlation signal strongly depend on which class of sources is responsible for the gamma-ray emission. If the DM contribution to the EGB is significant (at least in a definite energy range), although compatible with current observational bounds, its strong correlation with the cosmic shear makes such signal potentially detectable by combining Fermi Large Area Telescope data with forthcoming galaxy surveys, like the Dark Energy Survey and Euclid. At the same time, the same signal would demonstrate that the weak-lensing observables are indeed due to particle DM matter and not to possible modifications of general relativity.

  15. Biodistributions, Myelosuppression, and Toxicities in Mice Treated with an Anti-CD45 Antibody Labeled with the alpha-Emitting Radionuclides Bismuth-213 or Astatine-211

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Nakamae, Hirohisa; Wilbur, D. Scott; Hamlin, Donald K.

    2009-03-15

    We previously investigated 213Bi-labeled anti-CD45 antibody to replace total body irradiation as conditioning for hematopoietic cell transplantation in a canine model. While this allowed sustained engraftment of marrow, limited availability and high cost of 213Bi led to a preliminary investigation in mice of 211At-labeled antibody for the same application. To gain an understanding of the differences between the two radionuclides, biodistribution and myelosuppression/toxicity studies were conducted with 213Bi- and 211At-labeled rat anti-murine CD45 antibody, 30F11, conjugates. After injecting mice with 2-50 μCi on 10 μg 30F11 conjugate or 20 μCi on 2 or 40 μg conjugate, biodistributions, myelosuppression and non-hematologicalmore » toxicities were evaluated. Biodistribution studies showed that the spleen had the highest concentration of radioactivity, ranging from167-417 % injected dose/gram (%ID/g) at 24 h after injection in the 211At studies and 45-166 %ID/g at 3 h after injection in the 213Bi studies. The higher concentrations observed for 211At-labeled 30F11 was likely due to its longer half-life which, permitted more localization of antibody to the spleen before decay. 211At was more effective at myelosuppression for the same (mCi) quantity of injected radioactivity. Injection of only 20 or 50 μCi 211At resulted in lethal myeloablation. There was severe reversible acute hepatic toxicity with 50 μCi 213Bi, but not with lower doses or any dose of 211At. No significant renal toxicity occurred with either radionuclide. The data suggested that considerably lower quantities of 211At-labeled anti-CD45 antibody than 213Bi-labeled antibody might be effective for myelosuppression.« less

  16. Assessment of ileal function by abdominal counting of the retention of a gamma emitting bile acid analogue.

    PubMed Central

    Thaysen, E H; Orholm, M; Arnfred, T; Carl, J; Rødbro, P

    1982-01-01

    In eight patients without gastrointestinal complaints and 30 patients with various gastrointestinal disorders ileal bile acid conservation was assessed by oral administration of 75Se 23-selena-25-homocholic acid (SeHCAT) followed by abdominal gamma counting (SeHCAT-test). The results of the test correlated fairly well with the clinical features and with the [1-14C]-cholylglycine breath test including faecal 14C measurements (breath test). Of the two bile acid absorption tests the new is perhaps the more sensitive and is the one most easily performed. PMID:7117906

  17. A radioimmunoassay for ependymins beta and gamma: two goldfish brain proteins involved in behavioral plasticity.

    PubMed

    Schmidt, R; Shashoua, V E

    1981-04-01

    A radioimmunoassay (RIA) using 125I-labeled antigen was developed for the quantitative determination of two goldfish brain proteins (ependymins beta and gamma). The proteins were isolated from the cerebrospinal fluid (CSF) and cells of the ependymal zone surrounding goldfish brain ventricles. The turnover rates of beta and gamma were previously shown to be specifically enhanced after the animals successfully acquired a new pattern of swimming behavior. Femtomole quantities of ependymin beta were measurable by the RIA. In applications of the assay, beta and gamma ependymins were found to have common immunological properties, since 125I-beta-antigen bound to antibody could be displaced by unlabeled ependymin gamma as well as ependymin beta but not by a variety of other proteins including several purified glycoproteins isolated from goldfish brain. The ependymins were shown to constitute 14% of the total protein content of the brain extracellular fluid and also to be present as a minor component of the serum proteins (0.3%). Ependymins beta and gamma have an immunological reactivity in these fractions that can be increased by a factor of 30 on heating. The data suggest that the antigenicity of the molecules is highly masked, and that it may require some unraveling of the quaternary structure of the proteins before maximal interaction with the antisera becomes possible.

  18. Monitoring of left ventricular ejection fraction with a miniature, nonimaging nuclear detector: accuracy and reliability over time with special reference to blood labeling.

    PubMed

    Lindhardt, T B; Hesse, B; Gadsbøll, N

    1997-01-01

    The purpose of this study was to determine the accuracy of determinations of left ventricular ejection fraction (LVEF) by a nonimaging miniature nuclear detector system (Cardioscint) and to evaluate the feasibility of long-term LVEF monitoring in patients admitted to the coronary care unit, with special reference to the blood-labeling technique. Cardioscint LVEF values were compared with measurements of LVEF by conventional gamma camera radionuclide ventriculography in 33 patients with a wide range of LVEF values. In 21 of the 33 patients, long-term monitoring was carried out for 1 to 4 hours (mean 186 minutes), with three different kits: one for in vivo and two for in vitro red blood cell labeling. The stability of the labeling was assessed by determination of the activity of blood samples taken during the first 24 hours after blood labeling. The agreement between Cardioscint LVEF and gamma camera LVEF was good with automatic background correction (r = 0.82; regression equation y = 1.04x + 3.88) but poor with manual background correction (r = 0.50; y = 0.88x - 0.55). The agreement was highest in patients without wall motion abnormalities. The long-term monitoring showed no difference between morning and afternoon Cardioscint LVEF values. Short-lasting fluctuations in LVEFs greater than 10 EF units were observed in the majority of the patients. After 24 hours, the mean reduction in the physical decay-corrected count rate of the blood samples was most pronounced for the two in vitro blood-labeling kits (57% +/- 9% and 41% +/- 3%) and less for the in vivo blood-labeling kit (32% +/- 26%). This "biologic decay" had a marked influence on the Cardioscint monitoring results, demanding frequent background correction. A fairly accurate estimate of LVEF can be obtained with the nonimaging Cardioscint system, and continuous bedside LVEF monitoring can proceed for hours with little inconvenience to the patients. Instability of the red blood cell labeling during long

  19. Broad-Band Continuum and Line Emission of the gamma-Ray Blazar PKS 0537-441

    NASA Technical Reports Server (NTRS)

    Pian, E.; Falomo, R.; Hartman, R. C.; Maraschi, L.; Tavecchio, F.; Tornikoski, M.; Treves, A.; Urry, C. M.; Ballo, L.; Mukherjee, R.; hide

    2002-01-01

    PKS 0537-441, a bright gamma ray emitting blazar was observed at radio, optical, UV and X-ray frequencies during various EGRET paintings, often quasi-simultaneously. In 1995 the object was found in an intense emission state at all wavelengths. BeppoSAX observations made in 1998, non-simultaneously with exposures at other frequencies, allow us to characterize precisely the spectral shape of the high energy blazer component, which we attribute to inverse Compton scatter in The optical-to-gamma-ray spectral energy distributions at the different epochs show that the gamma-ray luminosity dominates the barometric output. This, together with the presence of optical and UV line emission, suggests that, besides the synchrotron self-Compton mechanism, the Compton upscattering of photons external to the jet (e.g., in the broad line region) may have a significant role for high energy radiation. The multiwavelength variability can be reproduced by changes of the plasma bulk Lorentz factor. The spectrum secured by ICE in 1995 appears to be partially absorbed shortward of approximately 1700 Angstroms. However, this signature is not detected in the HST spectrum taker during a lower state of the source. The presence of intervening absorbers is not supported by optical imaging and spectroscopy of the field.

  20. A low level of extragalactic background light as revealed by gamma-rays from blazars.

    PubMed

    Aharonian, F; Akhperjanian, A G; Bazer-Bachi, A R; Beilicke, M; Benbow, W; Berge, D; Bernlöhr, K; Boisson, C; Bolz, O; Borrel, V; Braun, I; Breitling, F; Brown, A M; Chadwick, P M; Chounet, L-M; Cornils, R; Costamante, L; Degrange, B; Dickinson, H J; Djannati-Ataï, A; Drury, L O'C; Dubus, G; Emmanoulopoulos, D; Espigat, P; Feinstein, F; Fontaine, G; Fuchs, Y; Funk, S; Gallant, Y A; Giebels, B; Gillessen, S; Glicenstein, J F; Goret, P; Hadjichristidis, C; Hauser, D; Hauser, M; Heinzelmann, G; Henri, G; Hermann, G; Hinton, J A; Hofmann, W; Holleran, M; Horns, D; Jacholkowska, A; de Jager, O C; Khélifi, B; Klages, S; Komin, Nu; Konopelko, A; Latham, I J; Le Gallou, R; Lemière, A; Lemoine-Goumard, M; Leroy, N; Lohse, T; Martin, J M; Martineau-Huynh, O; Marcowith, A; Masterson, C; McComb, T J L; de Naurois, M; Nolan, S J; Noutsos, A; Orford, K J; Osborne, J L; Ouchrif, M; Panter, M; Pelletier, G; Pita, S; Pühlhofer, G; Punch, M; Raubenheimer, B C; Raue, M; Raux, J; Rayner, S M; Reimer, A; Reimer, O; Ripken, J; Rob, L; Rolland, L; Rowell, G; Sahakian, V; Saugé, L; Schlenker, S; Schlickeiser, R; Schuster, C; Schwanke, U; Siewert, M; Sol, H; Spangler, D; Steenkamp, R; Stegmann, C; Tavernet, J-P; Terrier, R; Théoret, C G; Tluczykont, M; van Eldik, C; Vasileiadis, G; Venter, C; Vincent, P; Völk, H J; Wagner, S J

    2006-04-20

    The diffuse extragalactic background light consists of the sum of the starlight emitted by galaxies through the history of the Universe, and it could also have an important contribution from the 'first stars', which may have formed before galaxy formation began. Direct measurements are difficult and not yet conclusive, owing to the large uncertainties caused by the bright foreground emission associated with zodiacal light. An alternative approach is to study the absorption features imprinted on the gamma-ray spectra of distant extragalactic objects by interactions of those photons with the background light photons. Here we report the discovery of gamma-ray emission from the blazars H 2356 - 309 and 1ES 1101 - 232, at redshifts z = 0.165 and z = 0.186, respectively. Their unexpectedly hard spectra provide an upper limit on the background light at optical/near-infrared wavelengths that appears to be very close to the lower limit given by the integrated light of resolved galaxies. The background flux at these wavelengths accordingly seems to be strongly dominated by the direct starlight from galaxies, thus excluding a large contribution from other sources-in particular from the first stars formed. This result also indicates that intergalactic space is more transparent to gamma-rays than previously thought.