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Sample records for gardenia jasminoides ellis

  1. In vitro antioxidant activity of polysaccharide from Gardenia jasminoides ellis

    USGS Publications Warehouse

    Fan, Y.; Ge, Z.; Luo, A.

    2011-01-01

    A water-soluble polysaccharide, GP, was isolated from Gardenia jasminoides Ellis through hot water extraction followed by ethanol precipitation. The in vitro free radicals scavenging tests exhibited that GP has significant scavenging abilities especially for ABTS, DPPH, and hydroxyl radicals, which suggests that the polysaccharide GP is a novel antioxidant. ?? 2011 Academic Journals.

  2. Homogenate extraction of gardenia yellow pigment from Gardenia Jasminoides Ellis fruit using response surface methodology.

    PubMed

    Zhu, Xingyi; Mang, Yili; Shen, Fengqiong; Xie, Jie; Su, Weike

    2014-08-01

    Homogenate extraction technology was developed for extraction of gardenia yellow pigment from Gardenia jasminoides Ellis fruit. The operating parameters affecting the color value of gardenia yellow pigment were studied on the basis of a Box-Behnken design and response surface methodology. Results showed that the optimum extraction conditions were as follows: extraction time 41 s, ethanol concentration 50 %, ratio of liquid to material 15:1 (mL:g) and particle size 1.7 mm. Under the optimum condition, the experimental color value was 52.37 g(-1), which was in keeping with the predicted one. Compared with the heat extraction method, the color value of gardenia yellow pigment of homogenate extraction was higher and the extraction time was shorter. Homogenate extraction method is an ideal means for extraction of gardenia yellow pigment from Gardenia jasminoides Ellis fruit. PMID:25114350

  3. Guaiane-type sesquiterpenoid glucosides from Gardenia jasminoides Ellis.

    PubMed

    Yu, Yang; Gao, Hao; Dai, Yi; Xiao, Gao-Keng; Zhu, Hua-Jie; Yao, Xin-Sheng

    2011-05-01

    Two new guaiane-type sesquiterpenoid glucosides (1 and 2) were isolated from the fruit of Gardenia jasminoides Ellis. Their structures were elucidated to be (1R,7R,10S)-11-O-β-D-glucopyranosyl-4-guaien-3-one (1) and (1R,7R,10S)-7-hydroxy-11-O-β-D-glucopyranosyl-4-guaien-3-one (2) by one- and two-dimensional NMR techniques ((1)H NMR, (13)C NMR, HSQC, HMBC and NOESY), MS, CD spectrometry and chemical methods. PMID:21491483

  4. Chemical constituents from the fruits of Gardenia jasminoides Ellis.

    PubMed

    Yu, Yang; Feng, Xiao-Lin; Gao, Hao; Xie, Zuo-Lei; Dai, Yi; Huang, Xiao-Jun; Kurihara, Hiroshi; Ye, Wen-Cai; Zhong, Yi; Yao, Xin-Sheng

    2012-04-01

    A new lignan glucoside, (+)-(7S,8R,8'R)-lyoniresinol 9-O-β-D-(6″-O-trans-sinapoyl)glucopyranoside (1), and a new iridoid glucoside, 10-O-trans-sinapoylgeniposide (2), together with eight known compounds, were isolated from the fruits of Gardenia jasminoides Ellis. The structures of the isolates were elucidated by extensive spectroscopic studies, including UV, IR, 1D and 2D NMR, ESI-MS, HR-ESI-MS, and CD experiments. The short-term-memory-enhancement activities of some compounds were evaluated on an Aβ transgenic drosophila model. PMID:22245087

  5. Three new triterpenoid saponins from root of Gardenia jasminoides Ellis.

    PubMed

    Wang, Jing; Lu, Jincai; Lv, Chongning; Xu, Tanye; Jia, Lingyun

    2012-12-01

    Three new compounds (Gardeniside A-C), 11α,12α-epoxy-3β-[(O-β-D-glucuronopyranoside-6'-O-methly ester)oxy]olean-28,13-olide (1), siaresinolic acid 3-O-β-D-glucuronopyranoside-6'-O-methly ester (2), and 3-O-β-D- glucuronopyranoside-6'-O-methly ester-siaresinolic acid-28-O-β-D- glucopyranoside (3), with seven known compounds oleanolic acid 3-O-β-D- glucuronopyranoside-6'-O-methly ester (4), oleanolic acid 3-O-β-D- glucuropyranoside (5), hederagenin 3-O-β-D- glucuronopyranoside-6'-O- methly ester (6), chikusetsusaponin IVa methyl ester (7), chikusetsusaponin (8), chikusetsusaponin IVa butyl ester (9), siaresinolic acid 28-o-β-d-glucopyranosyl ester (10) were isolated from the root of Gardenia jasminoides Ellis. Six compounds (1, 4-7, and 9) showed cytotoxic activities against HeLa, A549, MCF-7 and A354-S2 cell lines. PMID:22796399

  6. Chemical fingerprinting of Gardenia jasminoides Ellis by HPLC-DAD-ESI-MS combined with chemometrics methods.

    PubMed

    Han, Yan; Wen, Jun; Zhou, Tingting; Fan, Guorong

    2015-12-01

    A fingerprint analysis method has been developed for characterization and discrimination of Gardenia jasminoides Ellis from different areas. The chemometrics methods including similarity evaluation, principal components analysis (PCA) and hierarchical clustering analysis (HCA) were introduced to identify more useful chemical markers for improving the quality control standard of dried ripe fruits of G. jasminoides Ellis. Then the selected chemical markers were analyzed by high performance liquid chromatography-diode array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) qualitatively and quantitatively. 23 characteristic peaks were assigned while 19 peaks of them were identified by comparing retention times, UV and MS spectra with authentic compounds or literature data. Moreover, 14 of them were determined quantitatively which could effectively evaluate the quality of G. jasminoides Ellis. This study was expected to provide comprehensive information for the quality evaluation of G. jasminoides Ellis, which would be a valuable reference for further study and development of this herb and related medicinal products. PMID:26041243

  7. Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers.

    PubMed

    Mei, Zhiqiang; Zhou, Boxu; Wei, Chunli; Cheng, Jingliang; Imani, Saber; Chen, Hanchun; Fu, Junjiang

    2015-01-01

    The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides. PMID:26569205

  8. Identification and quantification of constituents of Gardenia jasminoides Ellis (Zhizi) by HPLC-DAD-ESI-MS.

    PubMed

    Bergonzi, M C; Righeschi, C; Isacchi, B; Bilia, A R

    2012-09-15

    A simple, rapid and specific HPLC method was carried out for the analysis of characteristic constituents in Gardenia jasminoides Ellis (Zhizi), namely iridoids, caffeoyl quinic acid derivatives and crocins. The separation was successfully obtained using a C(18) column by gradient elution with mixtures of methanol and water as mobile phases; detection wavelength was set at 240 nm for iridoid glycosides, 315 nm for quinic acid derivatives and 438 nm for crocins. The analytical method was validated and the quantification of active compounds, namely iridoids, was performed. Linearity, precision, repeatability, stability, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were also reported. This assay was successfully applied for qualitative and quantitative analysis of five commercial samples of G. jasminoides Ellis. PMID:23107748

  9. Iridoids from Gardenia jasminoides.

    PubMed

    Ragasa, Consolacion Y; Pimenta, Leslie Elline N; Rideout, John A

    2007-10-01

    The dichloromethane extract of the air-dried flowers of Gardenia jasminoides Ellis. afforded a new iridoid natural product (1), and a diastereomeric mixture of two new iridoids (2a and 2b) in a 2 : 1 ratio. Their structures were elucidated by extensive 1D and 2D NMR spectroscopy. Antimicrobial tests on 1 indicated that it was moderately active against Candida albicans; slightly active against E. coli, Pseudomonas aeruginosa, Staphylococcus. aureus, and Trichophyton mentagrophytes; and inactive against Bacillus subtilis and Aspergillus niger.

  10. Effect of crocetin from Gardenia jasminoides Ellis on sleep: a pilot study.

    PubMed

    Kuratsune, H; Umigai, N; Takeno, R; Kajimoto, Y; Nakano, T

    2010-09-01

    Crocetin is a pharmacologically active carotenoid compound of Gardenia jasminoides Ellis used as a traditional herbal medicine and natural colorant. The present pilot study investigated the effect of crocetin on sleep. The clinical trial comprised a double-blind, placebo-controlled, crossover trial of 21 healthy adult men with a mild sleep complaint. It included two intervention periods of 2 weeks each, separated by a 2-week washout period. We measured objective sleep quality using an actigraph, and assessed the subjective symptoms using St Mary's Hospital Sleep Questionnaire. Actigraph data showed that after administration of crocetin, the number of wakening episodes was reduced compared to that of the placebo (p=0.025). Subjective data from St Mary's Hospital Sleep Questionnaire showed that crocetin tended to improve the quality of sleep compared to sleep before its intake. Additionally, no side effects from crocetin intake were observed. The results suggest that crocetin may contribute to improving the quality of sleep. PMID:20537515

  11. Fructus Gardenia (Gardenia jasminoides J. Ellis) phytochemistry, pharmacology of cardiovascular, and safety with the perspective of new drugs development.

    PubMed

    Liu, Hao; Chen, Yong-Feng; Li, Fang; Zhang, Hai-Yan

    2013-01-01

    The phytochemistry, cardiovascular pharmacology, toxicology, side effect, and further development prospects of Gardenia jasminoides J. Ellis (GJE) and its main constituents crocins and iridoid glycosides were studied. Numerous studies have confirmed that crocins and iridoid glycosides had effects of antioxidation, anti-inflammatory, anti-atherosclerosis, anti-ischemic brain injuries, anti-platelet aggregation, anti-hyperglycemia, anti-hyperlipidemia, anti-hypertension, and so on. Some of them might be related to several attractive pharmacodynamic actions of GJE such as promoting endothelium growth, protecting neurons, and inducing their differentiation. Both of them make it possible for GJE to prevent and cure thromboembolism and cardiovascular diseases well. From our own basic pharmacological research of GJE extract on several rat models, it has been known that GJE extract markedly prolonged bleeding time and inhibited platelet aggregation and thrombosis. It has significant proliferation effect on both endothelial cells and endothelial progenitor cells as well. As the mechanisms of GJE on those diseases were discussed and summarized, questions about its genetoxicity and hepatotoxicity were also discussed during its safety study to make the foundation for long-term medication and clinical research in the near future. PMID:23211013

  12. Gardenia jasminoides Ellis ethanol extract and its constituents reduce the risks of gastritis and reverse gastric lesions in rats.

    PubMed

    Lee, Je-Hyuk; Lee, Dong-Ung; Jeong, Choon-Sik

    2009-06-01

    In this study we investigated the effects of Gardenia jasminoides Ellis (GJE) extract and its constituents, such as ursolic acid and genipin, on gastritis in rats and the growth of human gastric cancer cells. The GJE extract, ursolic acid and genipin showed the acid-neutralizing capacities, the antioxidant activities, and the inhibitory effects on the growth of Helicobacter pylori (H. pylori), which are almost equivalent to positive control compounds. In addition, the GJE extract and ursolic acid had cytotoxic activity against AGS and SUN638 gastric cancer cells. The genipin and ursolic acid inhibited significant HCl/ethanol-induced gastric lesions. Taken together, GJE extract and its constituents might have antigastritic activities, associated with the antioxidant activities, acid-neutralizing capacities, and anti-H. pylori action. Also, we could suggest that genipin and ursolic acid may be useful for the treatment and/or protection of gastritis. PMID:19425231

  13. The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis

    PubMed Central

    Mat Taha, Rosna; Rashid, Kamaludin; Syafawati Yaacob, Jamilah

    2014-01-01

    The leaf of Gardenia jasminoides Ellis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L−1 with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L−1) and this amount was decreased from (84%) to (69%) when this media supplemented with Kinetin and TDZ (1 mg L−1) respectively were used. Leaf segments cultured on WPM media added with Kn (1 mg L−1) and TDZ (2 mg L−1) yielded the least amount of callus. It was found that WPM media added with IAA (4.5–5.0 mg L−1) were optimum for root induction from G. jasminoides plantlets. Antibacterial screening of leaf extracts (in vivo) showed no inhibitory effect against E. coli, P. aeruginosa, S. aureus, and B. cereus, in contrast to callus extracts from leaf cultures supplemented with NAA, which showed inhibition activity against E. coli and B. cereus. The callus extracts from leaf cultures grown on both MS and WPM media showed higher antioxidant and superoxide dismutase activities than leaf extracts. PMID:24967432

  14. The effect of various media and hormones via suspension culture on secondary metabolic activities of (Cape Jasmine) Gardenia jasminoides Ellis.

    PubMed

    Farzinebrahimi, Reza; Mat Taha, Rosna; Rashid, Kamaludin; Syafawati Yaacob, Jamilah

    2014-01-01

    The leaf of Gardenia jasminoides Ellis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L(-1) with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L(-1)) and this amount was decreased from (84%) to (69%) when this media supplemented with Kinetin and TDZ (1 mg L(-1)) respectively were used. Leaf segments cultured on WPM media added with Kn (1 mg L(-1)) and TDZ (2 mg L(-1)) yielded the least amount of callus. It was found that WPM media added with IAA (4.5-5.0 mg L(-1)) were optimum for root induction from G. jasminoides plantlets. Antibacterial screening of leaf extracts (in vivo) showed no inhibitory effect against E. coli, P. aeruginosa, S. aureus, and B. cereus, in contrast to callus extracts from leaf cultures supplemented with NAA, which showed inhibition activity against E. coli and B. cereus. The callus extracts from leaf cultures grown on both MS and WPM media showed higher antioxidant and superoxide dismutase activities than leaf extracts. PMID:24967432

  15. Rapid Antidepressant Activity of Ethanol Extract of Gardenia jasminoides Ellis Is Associated with Upregulation of BDNF Expression in the Hippocampus

    PubMed Central

    Zhang, Hailou; Xue, Wenda; Wu, Runjie; Gong, Tong; Tao, Weiwei; Zhou, Xin; Jiang, Jingjing; Zhang, Ying; Zhang, Nan; Cui, Yi; Chen, Chang; Chen, Gang

    2015-01-01

    Ethanol extract of Yueju pill, a Traditional Chinese Medicine herbal formula widely used to treat mood disorders, demonstrates rapid antidepressant effects similar to ketamine, likely via instant enhancement of brain-derived neurotrophic factor (BDNF) expression in the hippocampus. Here we investigated ethanol extracts of the constituent herbs of Yueju responsible for rapid antidepressant effects. Screening with tail suspension test in Kunming mice at 24 hours after a single administration of five individual constituent herbs of Yueju, we found that only Gardenia jasminoides Ellis (GJ) showed a significant effect. The antidepressant response started at 2 hours after GJ administration. Similar to Yueju and ketamine, a single administration of GJ significantly reduced the number of escape failures in the learned helplessness test. Furthermore, GJ decreased latency of food consumption in the novelty suppressed-feeding test. Additionally, starting from 2 hours and continuing for over 20 hours after GJ administration, BDNF expression in the hippocampus was upregulated, temporally linked with the antidepressant response. These findings suggest that GJ has rapid antidepressant effects, which are associated with the elevated expression of BDNF in the hippocampus. In Yueju formula, Yue represents GJ, as thus our study demonstrates the primary role of GJ in rapid antidepressant efficacy of Yueju. PMID:25878718

  16. Crocetin, a carotenoid from Gardenia jasminoides Ellis, protects against hypertension and cerebral thrombogenesis in stroke-prone spontaneously hypertensive rats.

    PubMed

    Higashino, Saori; Sasaki, Yasuto; Giddings, John C; Hyodo, Kanae; Sakata, Shigeko Fujimoto; Matsuda, Koichi; Horikawa, Yoko; Yamamoto, Junichiro

    2014-09-01

    Crocetin is a natural carotenoid dicarboxylic acid that is found in the fruit of Gardenia jasminoides Ellis (Cape Jasmine) and in the stamen and pistil of Crocus sativus L. (saffron). It is used worldwide as an important spice, food colorant, and herbal medicine. In the current investigation, we have examined the cardiovascular effects of crocetin using stroke-prone spontaneously hypertensive rats (SHRSPs). Male SHRSPs (6 weeks old) were classified into three groups: a control group and two crocetin groups (25 and 50 mg/kg/day). The animals were given crocetin for 3 weeks. Body weights in each group were not significantly different during the treatment period, but the increase in systolic blood pressures observed with age was significantly moderated by crocetin. Thrombogenesis, assessed using a He-Ne laser technique in pial vessels, was significantly decreased. Antioxidant activity, assessed by measuring urinary 8-hydroxy-2'-deoxyguanosine levels, together with urinary nitric oxide (NO) metabolite levels, was increased significantly after treatment. Acetylcholine-induced vasodilation was measured using the aorta and indicated that endothelial function was significantly improved by crocetin. These results strongly suggest that the antihypertensive and antithrombotic effects of crocetin were related to an increase in bioavailable NO, possibly mediated by decreased inactivation of NO by reactive oxygen species. PMID:24550159

  17. Genipin, a constituent of Gardenia jasminoides Ellis, induces apoptosis and inhibits invasion in MDA-MB-231 breast cancer cells.

    PubMed

    Kim, Eun-Sook; Jeong, Choon-Sik; Moon, Aree

    2012-02-01

    Genipin, a constituent of Gardenia jasminoides Ellis, is used in the treatment of hepatic disorders and inflammatory diseases in traditional medicine. Although mounting evidence suggests an anti-tumor activity of genipin in several cancer cell systems, the inhibitory effect of genipin on the growth of breast cancer cells has not been reported yet. The present study aimed to investigate the anti-proliferative activity of genipin in MDA-MB-231 human breast cancer cells. Herein, we showed that genipin efficiently induced apoptosis in MDA-MB-231 cells by the down-regulation of Bcl-2, up-regulation of Bax and proteolytic activation of caspase-3. Activation of JNK and p38 MAPK also increased by genipin. Importantly, genipin significantly inhibited invasive and migratory phenotypes of MDA-MB-231 cells. Taken together, this study demonstrates that genipin induces apoptosis and inhibits invasive/migratory abilities of highly invasive MDA-MB-231 human breast cancer cells, suggesting a potential application of genipin as a chemopreventive agent that may prevent or alleviate metastatic breast cancer. PMID:22020372

  18. The biosynthesis of palladium nanoparticles by antioxidants in Gardenia jasminoides Ellis: long lifetime nanocatalysts for p-nitrotoluene hydrogenation.

    PubMed

    Jia, Lishan; Zhang, Qian; Li, Qingbiao; Song, Hao

    2009-09-23

    Gardenia jasminoides Ellis' water crude extract was used for the bioreduction of palladium chloride in this paper. The UV-vis spectrum, x-ray diffraction spectrum measurement, the Fourier transform infrared spectroscopy and TEM technique confirmed the formation of palladium nanoparticles and identified antioxidants including geniposide, chlorogenic acid, crocins and crocetin were reducing and stabilizing agents for synthesizing palladium nanoparticles in water crude extract. The particle size and dispersity were temperature-dependent. The particle sizes ranged from 3 to 5 nm and revealed the best dispersity at 70 degrees C. Catalytic performance of the biosynthetic Pd nanoparticles with good dispersity was investigated by hydrogenation of p-nitrotoluene. The catalysts showed a conversion of 100% under conditions of 5 MPa, 150 degrees C for 2 h. The selectivity of p-methyl-cyclohexylamine achieved 26.3%. The catalyst was recycled five times with no agglomeration and maintained activity, which was attributed to the appropriate protection of the antioxidants. On the basis of the study, it appears to be a new promising biosynthetic nanocatalyst for the development of an industrial process. PMID:19713585

  19. The biosynthesis of palladium nanoparticles by antioxidants in Gardenia jasminoides Ellis: long lifetime nanocatalysts for p-nitrotoluene hydrogenation

    NASA Astrophysics Data System (ADS)

    Jia, Lishan; Zhang, Qian; Li, Qingbiao; Song, Hao

    2009-09-01

    Gardenia jasminoides Ellis' water crude extract was used for the bioreduction of palladium chloride in this paper. The UV-vis spectrum, x-ray diffraction spectrum measurement, the Fourier transform infrared spectroscopy and TEM technique confirmed the formation of palladium nanoparticles and identified antioxidants including geniposide, chlorogenic acid, crocins and crocetin were reducing and stabilizing agents for synthesizing palladium nanoparticles in water crude extract. The particle size and dispersity were temperature-dependent. The particle sizes ranged from 3 to 5 nm and revealed the best dispersity at 70 °C. Catalytic performance of the biosynthetic Pd nanoparticles with good dispersity was investigated by hydrogenation of p-nitrotoluene. The catalysts showed a conversion of 100% under conditions of 5 MPa, 150 °C for 2 h. The selectivity of p-methyl-cyclohexylamine achieved 26.3%. The catalyst was recycled five times with no agglomeration and maintained activity, which was attributed to the appropriate protection of the antioxidants. On the basis of the study, it appears to be a new promising biosynthetic nanocatalyst for the development of an industrial process.

  20. Rapid Antidepressant Activity of Ethanol Extract of Gardenia jasminoides Ellis Is Associated with Upregulation of BDNF Expression in the Hippocampus.

    PubMed

    Zhang, Hailou; Xue, Wenda; Wu, Runjie; Gong, Tong; Tao, Weiwei; Zhou, Xin; Jiang, Jingjing; Zhang, Ying; Zhang, Nan; Cui, Yi; Chen, Chang; Chen, Gang

    2015-01-01

    Ethanol extract of Yueju pill, a Traditional Chinese Medicine herbal formula widely used to treat mood disorders, demonstrates rapid antidepressant effects similar to ketamine, likely via instant enhancement of brain-derived neurotrophic factor (BDNF) expression in the hippocampus. Here we investigated ethanol extracts of the constituent herbs of Yueju responsible for rapid antidepressant effects. Screening with tail suspension test in Kunming mice at 24 hours after a single administration of five individual constituent herbs of Yueju, we found that only Gardenia jasminoides Ellis (GJ) showed a significant effect. The antidepressant response started at 2 hours after GJ administration. Similar to Yueju and ketamine, a single administration of GJ significantly reduced the number of escape failures in the learned helplessness test. Furthermore, GJ decreased latency of food consumption in the novelty suppressed-feeding test. Additionally, starting from 2 hours and continuing for over 20 hours after GJ administration, BDNF expression in the hippocampus was upregulated, temporally linked with the antidepressant response. These findings suggest that GJ has rapid antidepressant effects, which are associated with the elevated expression of BDNF in the hippocampus. In Yueju formula, Yue represents GJ, as thus our study demonstrates the primary role of GJ in rapid antidepressant efficacy of Yueju. PMID:25878718

  1. Fragmentation study of iridoid glycosides including epimers by liquid chromatography-diode array detection/electrospray ionization mass spectrometry and its application in metabolic fingerprint analysis of Gardenia jasminoides Ellis.

    PubMed

    Zhou, Tingting; Liu, Hua; Wen, Jun; Fan, Guorong; Chai, Yifeng; Wu, Yutian

    2010-09-15

    A high-performance liquid chromatography-diode array detection/electrospray ionization mass spectrometry (HPLC-DAD/ESI-MS) method was applied to the characterization of ten iridoid glycosides in Gardenia jasminoides Ellis, a traditional Chinese medicine. During the process of structural elucidation, two groups of isomers including two epimers were structurally characterized and differentiated according to their distinctive fragmentation patterns which were closely related to their isomeric differentiations. Subsequently, the major compounds were purified by multi-dimensional chromatography and semi-preparative HPLC and the structure identification was confirmed with NMR techniques. The major fragmentation pathways of iridoid glycosides in Gardenia jasminoides Ellis obtained through the MS data were schemed systematically, which provided the best sensitivity and specificity for characterization of the iridoid glycosides especially the isomers so far. Based on the fragmentation patterns of iridoid glycosides concluded, seven major iridoid glycosides were characterized in rat plasma after intravenous administration of Gardenia jasminoides Ellis. PMID:20740525

  2. Large-scale isolation and purification of geniposide from the fruit of Gardenia jasminoides Ellis by high-speed counter-current chromatography.

    PubMed

    Zhou, Tingting; Fan, Guorong; Hong, Zhanying; Chai, Yifeng; Wu, Yutian

    2005-12-23

    High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of geniposide from Gardenia jasminoides Ellis. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate-n-butanol-water (2:1:3, v/v/v). According to the above solvent system, preparative HSCCC was successfully performed with the optimal solvent system composed of ethyl acetate-n-butanol-water (2:1.5:3, v/v/v) yielding 389 mg of geniposide at over 98% purity from 1g of the partially purified extract with 38.9% recovery in a one-step separation.

  3. Profiling of components and validated determination of iridoids in Gardenia Jasminoides Ellis fruit by a high-performance-thin-layer- chromatography/mass spectrometry approach.

    PubMed

    Coran, Silvia A; Mulas, Stefano; Vasconi, Alessio

    2014-01-17

    A novel method was set up with the aim to obtain a simultaneous cross comparative evaluation of different Gardenia Jasminoides Ellis fruits by the HPTLC fingerprint approach. The main components among the iridoid, hydroxycinnamic derivative and crocin classes were identified by TLC-MS ancillary techniques. The iridoids geniposide, gardenoside and genepin-1-β-d-gentiobioside were also quantitated by densitometric scanning at 240nm. LiChrospher HPTLC Silica gel 60 RP-18 W F254, 20cm×10cm plates with acetonitrile: formic acid 0.1% (40:60 v/v) as the mobile phase was used. The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Iridoids were quantified in the range of 240-1140ng with RSD of repeatability and intermediate precision between 0.9-2.5% and accuracy with bias 1.6-2.6%. The method was tested on six commercial Gardenia Jasminoides fruit samples.

  4. Profiling of components and validated determination of iridoids in Gardenia Jasminoides Ellis fruit by a high-performance-thin-layer- chromatography/mass spectrometry approach.

    PubMed

    Coran, Silvia A; Mulas, Stefano; Vasconi, Alessio

    2014-01-17

    A novel method was set up with the aim to obtain a simultaneous cross comparative evaluation of different Gardenia Jasminoides Ellis fruits by the HPTLC fingerprint approach. The main components among the iridoid, hydroxycinnamic derivative and crocin classes were identified by TLC-MS ancillary techniques. The iridoids geniposide, gardenoside and genepin-1-β-d-gentiobioside were also quantitated by densitometric scanning at 240nm. LiChrospher HPTLC Silica gel 60 RP-18 W F254, 20cm×10cm plates with acetonitrile: formic acid 0.1% (40:60 v/v) as the mobile phase was used. The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Iridoids were quantified in the range of 240-1140ng with RSD of repeatability and intermediate precision between 0.9-2.5% and accuracy with bias 1.6-2.6%. The method was tested on six commercial Gardenia Jasminoides fruit samples. PMID:24365117

  5. Simple and effective large-scale preparation of geniposide from fruit of Gardenia jasminoides Ellis using a liquid-liquid two-phase extraction.

    PubMed

    Zhou, Min; Zhuo, Jiaxiong; Wei, Wanxing; Zhu, Jianwen; Ling, Xiurong

    2012-12-01

    Geniposide was prepared on a large-scale using a selective two-phase liquid-liquid extraction. The aqueous residue from the fruit of Gardenia jasminoides Ellis was treated with sodium carbonate and extracted with n-butanol several times. The n-butanol extracts were treated with activated granular charcoal to remove pigments and were then concentrated to produce a residue with a high solid content. The residue was crystallized to obtain geniposide with 98% purity. For large-scale synthesis, the residue (solid content 45%, geniposide 5.5%) was extracted to generate 70g of geniposide with 98% purity and 84.8% recovery using 1500g residue. PMID:22975161

  6. Identification of a new anti-LPS agent, geniposide, from Gardenia jasminoides Ellis, and its ability of direct binding and neutralization of lipopolysaccharide in vitro and in vivo.

    PubMed

    Zheng, Xinchuan; Yang, Dong; Liu, Xin; Wang, Ning; Li, Bin; Cao, Hongwei; Lu, Yongling; Wei, Guo; Zhou, Hong; Zheng, Jiang

    2010-10-01

    Lipopolysaccharide (LPS/endotoxin) is a key pathogen recognition molecule for sepsis. Currently, one of the therapeutic approaches for severe sepsis is focusing on the neutralization of LPS, and clinical trials have shown a lot of traditional Chinese herbs possess anti-sepsis function. Herein, to elucidate the bioactive components of traditional Chinese herbs that can neutralize LPS, the lipid A-binding abilities of sixty herbs were tested using affinity biosensor technology. The aqueous extract of Gardenia jasminoides Ellis, traditionally used to treat inflammation in Asian countries for centuries, was further investigated. Subsequently, a monomer, identified as geniposide, was isolated. In vitro, geniposide was found to directly bind LPS and neutralize LPS. It dose-dependently inhibited cytokines release from RAW264.7 cells induced by LPS without affecting the cell viability, and inhibited TNF-α mRNA expression up-regulated by LPS. However, geniposide did not decrease TNF-α release induced by CpG DNA, Poly I:C or IL-1β. Significantly, geniposide dose-dependently down-regulated TLR4 mRNA expression up-regulated by LPS, and suppressed the phosphorylations of p38 MAKP induced by LPS but not by IL-1β. In vivo, geniposide (40mg/kg) could significantly protect mice challenge with lethal heat-killed E. coli, and dose-dependently decreased the level of serum endotoxin which was tightly associated with the cytokine levels in endotoxemia mice. In summary, we successfully isolated geniposide from G. jasminoides Ellis. Geniposide directly bound LPS and neutralized LPS in vitro, and significantly protected sepsis model mice. Therefore, geniposide could be as a useful lead compound for anti-sepsis drug development. PMID:20655404

  7. Acousto-optic tunable filter near-infrared spectroscopy for in-line monitoring liquid-liquid extraction of Gardenia jasminoides Ellis based on statistical analysis.

    PubMed

    Wu, Sha; Jin, Ye; Liu, Qi-An; Wu, Jian-Xiong; Bi, Yu-An; Wang, Zhen-Zhong; Xiao, Wei

    2015-10-01

    This study aimed to monitor liquid-liquid extraction of Gardenia jasminoides Ellis (Zhizi in Chinese) using in-line near-infrared spectroscopy. Shanzhiside (SZS), deacetyl asperulosidic acid methyl ester (DAAME), genipin-1-β-D-gentiobioside (GG), geniposide (GS), total acids (TA) and soluble solid content (SSC) were selected as quality control indicators, and measured by reference methods. Both partial least-squares regression (PLSR) and back propagation artificial neural networks (BP-ANN) were applied to create models to predict the content of above indicators. Paired-samples t-test and nonparametric test were used to compare differences in predictive values between two models of each indicator. Relative standard error of prediction (RSEP) and mean absolute percentage error (MAPE) were used to evaluate the predictive accuracy of the established models. The results showed that there was no significant difference in predicting DAAME, GS and TA between two models. However, PLSR model gave better accuracy in predicting GG and SZS than BP-ANN model. The BP-ANN model of SSC was better than PLSR model. This study shows that NIR spectroscopy can be used for rapid and accurate analysis of quality control indicators in the liquid-liquid extraction of Zhizi. Simultaneously, this study can serve as technical support for the application of NIR spectroscopy in the industrial production process. PMID:26601419

  8. Ultrasound-assisted extraction of geniposide from Gardenia jasminoides.

    PubMed

    Wang, Xin-Sheng; Wu, Yan-Fang; Dai, Shi-Lin; Chen, Rong; Shao, Ying

    2012-11-01

    Fructus gardeniae (Zhizi), one of commonly-used traditional Chinese medicines, is derived from the ripe fruit of the evergreen shrub, Gardenia jasminoides Ellis, and is an ingredient of many traditional Chinese preparations, and has numerous pharmacological actions. Geniposide is the important bioactive element derived from F. gardeniae. This study established optimum conditions and method of ultrasound-assisted extraction for geniposide from F. gardeniae by exploring the different experimental parameters, such as type of solvent, ratio of solid/liquid, extraction temperature and extraction time. The data gained from this study is important to further extract and apply the geniposide and is also a significant reference to extract the bioactive compounds from plant materials by the ultrasound-assisted method. PMID:22579801

  9. Crocin and geniposide profiles and radical scavenging activity of gardenia fruits (Gardenia jasminoides Ellis) from different cultivars and at the various stages of maturation.

    PubMed

    Chen, Yang; Zhang, Hao; Li, Yi-Xin; Cai, Le; Huang, Juan; Zhao, Can; Jia, Lin; Buchanan, Ryan; Yang, Ting; Jiang, Li-Juan

    2010-06-01

    The major components of gardenia fruits are geniposide and water soluble pigment crocins. In this study, we investigate crocins and geniposide profiles of gardenia fruits from different cultivars and at the various stages of maturation. DPPH scavenging activity of gardenia fruits from different cultivars and at the various stages of fruit maturation was also assayed. Quantitative determination of crocins in the gardenia at the various stages of maturation revealed a significant increase when ripening. However, geniposide content was negatively correlated with ripening stages. A significant difference was observed when comparing crocin content of different gardenia from various cultivars and geniposide content also showed marked variety. Current study indicated no relationship between crocin and geniposide content in gardenia fruits at the various stages of maturation and DPPH radical scavenging activity. Data showed that, although crocins feature markedly less DPPH scavenging activity than gardenia ethanol extract, total crocin content of gardenias collected in various cultivars correlate, to a certain degree, with radical scavenging effects of the Chinese traditional medicine (r=0.75). PMID:19815056

  10. Bioactivity-guided fractionation and analysis of compounds with anti-influenza virus activity from Gardenia jasminoides Ellis.

    PubMed

    Yang, Quanjun; Wu, Bin; Shi, Yujing; Du, Xiaowei; Fan, Mingsong; Sun, Zhaolin; Cui, Xiaolan; Huang, Chenggang

    2012-01-01

    Bioassay-guided fractionation of extracts from Fructus Gardeniae led to analysis of its bioactive natural products. After infection by influenza virus strain A/FM/1/47-MA in vivo, antiviral activity of the extracts were investigated. The target fraction was orally administered to rats and blood was collected. High-performance liquid chromatography coupled with photo diode array detector and electrospray ion trap multiple-stage tandem mass spectrometry was applied to screen the compounds absorbed into the blood. A structural characterization based on the retention time, ultraviolet spectra, parent ions and fragmentation ions was performed. Thirteen compounds were confirmed or tentatively identified. This provides an accurate profile of the composition of bioactive compounds responsible for the anti-influenza properties. PMID:22297738

  11. Geniposide, from Gardenia jasminoides Ellis, inhibits the inflammatory response in the primary mouse macrophages and mouse models.

    PubMed

    Fu, Yunhe; Liu, Bo; Liu, Jinhua; Liu, Zhicheng; Liang, Dejie; Li, Fengyang; Li, Depeng; Cao, Yongguo; Zhang, Xichen; Zhang, Naisheng; Yang, Zhengtao

    2012-12-01

    Geniposide, a main iridoid glucoside component of gardenia fruit, has been known to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The objective of this study was to investigate the protective effects of geniposide on inflammation in lipopolysaccharide (LPS) stimulated primary mouse macrophages in vitro and LPS induced lung injury model in vivo. The expression of pro-inflammatory cytokines was determined by enzyme-linked immunosorbent assay (ELISA). Nuclear factor-kappa B (NF-κB), inhibitory kappa B (IκBα) protein, p38, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and Toll-like receptor 4 (TLR4) were determined by Western blot. Further analysis was carried out in mTLR4 and mMD-2 co-transfected HEK293 cells. The results showed that geniposide markedly inhibited the LPS-induced TNF-α, IL-6 and IL-1β production both in vitro and in vivo. Geniposide blocked the phosphorylation of IκBα, p65, p38, ERK and JNK in LPS stimulated primary mouse macrophages. Furthermore, geniposide inhibited the expression of TLR4 in LPS stimulated primary mouse macrophages and inhibited the LPS-induced IL-8 production in HEK293-mTLR4/MD-2 cells. In vivo study, it was also observed that geniposide attenuated lung histopathologic changes in the mouse models. These results suggest that geniposide exerts an anti-inflammatory property by down-regulating the expression of TLR4 up-regulated by LPS. Geniposide is highly effective in inhibiting acute lung injury and may be a promising potential therapeutic reagent for acute lung injury treatment. PMID:22878137

  12. Biomass assisted synthesis of alumina by Gardenia Jasminoides Ellis and their application for removal of Ni(II) from aqueous solution.

    PubMed

    Zheng, Nan; Zhao, Yusheng; Song, Qianqian; Jia, Lishan; Fang, Weiping

    2013-09-15

    A simple and novel process has been proposed to synthesize alumina using gardenia extract and aluminum salts in an aqueous solution. The alumina sample notated as "bio-Al₂O₃" was characterized by X-ray diffraction (XRD) and nitrogen adsorption-desorption experiment. The results indicated that the existence of the gardenia biomass enlarged the surface area of alumina and reached 256 m(2)/g. The thermo gravimetric (TG), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) results showed that gardenia biomass bound to the surface of the alumina has substantially improved the adsorption capacity of Ni(II) and the adsorption behavior of nickel ion was related to the biomass functional groups. The results of three adsorption-desorption cycles showed that the bio-Al₂O₃ using as the adsorbent for Ni(II) was relatively stable. The kinetic of the Ni(II) adsorption by the bio-Al₂O₃ followed pseudo-second-order equation. Langmuir and Freundlich isotherm models were applied to analyze the experimental data and the result demonstrated that the adsorption isotherms followed Langmuir isotherm model. PMID:23892172

  13. The Accumulation of Crocin and Geniposide and Transcripts of Phytoene Synthase during Maturation of Gardenia jasminoides Fruit

    PubMed Central

    Zhu, Bi-Yun

    2013-01-01

    Gardenia fruit (fruit of Gardenia jasminoides Ellis) is used as a natural pigment resource and a Chinese traditional medicine. The white mesocarp turning orange or red that occurs during gardenia fruit maturation arises from the production and accumulation of the apocarotenoids, especially crocin-1, which is derived from carotenoid. Meanwhile, the major medical component geniposide is accumulated in gardenia fruit. To further our understanding of the synthetic and accumulation mechanism for crocin-1 and geniposide in gardenia fruit, the contents of crocin-1 and geniposide and the transcripts of phytoene synthase (GjPSY) profiles in gardenia fruits were examined at various stages of maturation. The concentration of crocin-1 and geniposide in gardenia fruit was determined by reversed-phase high-performance liquid chromatography (HPLC). The results showed that the concentration of crocetin-1 was increased during fruit development and the concentration of geniposide does not change significantly during maturing. The expression levels of GjPSY mRNA were examined by RT-PCR. It was revealed that GjPSY was constitutively expressed during fruit development, suggesting that the primary mechanism that controls crocin accumulation in G. jasminoides fruits during development is not correlated to the differential regulation of transcript levels of GjPSY gene. PMID:23634173

  14. The Accumulation of Crocin and Geniposide and Transcripts of Phytoene Synthase during Maturation of Gardenia jasminoides Fruit.

    PubMed

    Gao, Lan; Zhu, Bi-Yun

    2013-01-01

    Gardenia fruit (fruit of Gardenia jasminoides Ellis) is used as a natural pigment resource and a Chinese traditional medicine. The white mesocarp turning orange or red that occurs during gardenia fruit maturation arises from the production and accumulation of the apocarotenoids, especially crocin-1, which is derived from carotenoid. Meanwhile, the major medical component geniposide is accumulated in gardenia fruit. To further our understanding of the synthetic and accumulation mechanism for crocin-1 and geniposide in gardenia fruit, the contents of crocin-1 and geniposide and the transcripts of phytoene synthase (GjPSY) profiles in gardenia fruits were examined at various stages of maturation. The concentration of crocin-1 and geniposide in gardenia fruit was determined by reversed-phase high-performance liquid chromatography (HPLC). The results showed that the concentration of crocetin-1 was increased during fruit development and the concentration of geniposide does not change significantly during maturing. The expression levels of GjPSY mRNA were examined by RT-PCR. It was revealed that GjPSY was constitutively expressed during fruit development, suggesting that the primary mechanism that controls crocin accumulation in G. jasminoides fruits during development is not correlated to the differential regulation of transcript levels of GjPSY gene. PMID:23634173

  15. Volatile profile and sensory property of Gardenia jasminoides aroma extracts.

    PubMed

    Kanlayavattanakul, Mayuree; Lourith, Nattaya

    2015-01-01

    The volatile profiles of aroma extracts prepared from the flower of Gardenia jasminoides by different methods were investigated using gas chromatography-mass spectrometry. The enfleurage extraction using spermaceti wax and palm oil afforded the best aroma extract with a preference that was significantly (p < 0.05) better than those from solvent extractions, as sensorially evaluated in 43 volunteers. The odor quality of the absolute de enfleurage was similar to the floral scent of fresh gardenia, as confirmed in 152 volunteers. Although female volunteers were insignificantly (p > 0.05) better sensed than male volunteers, age was significant (p < 0.05). The nuance gardenia floral scent was contributed by farnesene, Z-3-hexenyl tiglate, Z-3-hexenyl benzoate, and indole. The relaxing and refreshing sensations of the gardenia odor suggest its applications in body care, cleansing products, and perfume. This study addresses the increasing interest in floral fragrances. The aroma profile and sensory property of this sweet and elegant scent flower will strengthen and expand the applications of gardenia from traditional medicine to those of perfumery and the field of phytochemistry.

  16. Volatile profile and sensory property of Gardenia jasminoides aroma extracts.

    PubMed

    Kanlayavattanakul, Mayuree; Lourith, Nattaya

    2015-01-01

    The volatile profiles of aroma extracts prepared from the flower of Gardenia jasminoides by different methods were investigated using gas chromatography-mass spectrometry. The enfleurage extraction using spermaceti wax and palm oil afforded the best aroma extract with a preference that was significantly (p < 0.05) better than those from solvent extractions, as sensorially evaluated in 43 volunteers. The odor quality of the absolute de enfleurage was similar to the floral scent of fresh gardenia, as confirmed in 152 volunteers. Although female volunteers were insignificantly (p > 0.05) better sensed than male volunteers, age was significant (p < 0.05). The nuance gardenia floral scent was contributed by farnesene, Z-3-hexenyl tiglate, Z-3-hexenyl benzoate, and indole. The relaxing and refreshing sensations of the gardenia odor suggest its applications in body care, cleansing products, and perfume. This study addresses the increasing interest in floral fragrances. The aroma profile and sensory property of this sweet and elegant scent flower will strengthen and expand the applications of gardenia from traditional medicine to those of perfumery and the field of phytochemistry. PMID:27125012

  17. Immunosuppressive iridoids from the fruits of Gardenia jasminoides.

    PubMed

    Chang, Wen-Liang; Wang, Hua-Ying; Shi, Li-Shian; Lai, Jenn-Haung; Lin, Hang-Ching

    2005-11-01

    A new iridoid, gardaloside (1), and a new safranal-type monoterpene, jasminoside G (2), together with 10 known compounds including nine iridoids and a second safranal-type monoterpene, were isolated from the fruits of Gardenia jasminoides. The structures of 1 and 2 were established on the basis of spectroscopic evidence. Of these compounds, geniposide (3), 6alpha-hydroxygeniposide (5), ixoroside (7), and shanzhiside (8) showed significant inhibition of IL-2 secretion by phorbol myristate acetate and anti-CD28 monoclonal antibody co-stimulated activation of human peripheral blood T cells.

  18. 2-methyl-L-erythritol glycosides from Gardenia jasminoides.

    PubMed

    Yang, Liguo; Peng, Kaifeng; Zhao, Shizhe; Zhao, Feng; Chen, Lixia; Qiu, Feng

    2013-09-01

    Two new glycosides, 2-methyl-L-erythritol-4-O-(6-O-trans-sinapoyl)-β-D-glucopyranoside (1) and 2-methyl-L-erythritol-1-O-(6-O-trans-sinapoyl)-β-D-glucopyranoside (2), along with two known triterpenoids (3-4), four quinic acid derivatives (5-8) and one flavonoid (9) were isolated from the fruit of Gardenia jasminoides. Their structures were elucidated through MS and 2D NMR experiments (HMQC and HMBC). Inhibitory effects of the isolated compounds on nitric oxide production in lipopolysaccharide-activated macrophages were evaluated. Though 2-methyl-D-erythritol and its glycosides have been reported in a few references, this is the first report about 2-methyl-L-erythritol glycosides. Based on this finding, we propose that 2-methyl-L-erythritol might be a new intermediate in the non-mevalonate biosynthesis of terpenoids.

  19. Multiplication végétative in vitro de Gardenia jasminoïdes Ellis: In vitro Vegetative Multiplication of Gardenia jasminoïdes Ellis.

    PubMed

    Dumanois, C; Godin, B; Bigot, C

    1984-11-01

    The vegetative propagation in vitro of Gardenia jasminoides Ellis, an ornamental shrub producing secondary metabolites useful in perfumery and pharmacy, has been investigated. In the presence of benzylaminopurine (0.3 to 1 mg l(-1)) and indoleacetic acid (1 mg l(-1)), axillary shoots arose from nodes of greenhause plants, but a decrease of morphogenic activity was noticed in subcultures. Mother plants have been developed in vitro from which shoots may be taken with the most effective system being a monthly subculture of nodal explants. As many as one million shoots per year could, theoretically, be produced from one shoot grown in vitro. Rooting occurred easily in three weeks in vitro in the presence of indoleacetic acid (1 mg l(-1)) and charcoal (2 g l(-1)) or in vivo by soaking the base of the shoot in the same auxin for two hours. After acclimatization, the rooted plants developed and flowered normally. The successive stages have been sufficiently well monitored to be used as an effective means of vegetative propagation. PMID:23195380

  20. [Establishment of hepatocyte extraction combined with HPLC(HE-HPLC) and application in analysis of active components in the fruits of Gardenia jasminoides extract].

    PubMed

    Hong, Min; Ma, Hongyu; Zhu, Quan

    2009-02-01

    To screen effective principles from traditional Chinese medicine, a method named hepatocyte extraction combined with HPLC (HE-HPLC) was establish in this study. The active principles in the fruits of Gardenia jasminoides Ellis extract were combined with the hepatocytes under imitated physiological environments. Then the unattached substances were washed off by PBS with pH 7.4. After that the conjugated compounds were eluted by PBS with pH 4.0. These compounds released from target sites were collected and handled through SPE to be condensed, and analyzed by HPLC. The results indicated that two characteristic active compounds in the fruits of G. jasminoides extract binded to the hepatocytes. One of them is geniposide. The other is continued to be identified. It is showed that active principles which could bind to hepotocyte (through receptors, Channels, enzymes, etc.) could be detected, at least partly, by HE-HPLC analysis. There was a significant correlation between the retention properties of the active compounds which was obtained by HE-HPLC and their pharmacological effects on hepotocytes. PMID:19459310

  1. HPLC-Analysis of Polyphenolic Compounds in Gardenia jasminoides and Determination of Antioxidant Activity by Using Free Radical Scavenging Assays

    PubMed Central

    Uddin, Riaz; Saha, Moni Rani; Subhan, Nusrat; Hossain, Hemayet; Jahan, Ismet Ara; Akter, Raushanara; Alam, Ashraful

    2014-01-01

    Purpose: Gardenia jasminoides is a traditional medicinal plant rich in anti-inflammatory flavonoids and phenolic compounds and used for the treatment of inflammatory diseases and pain. In this present study, antioxidant potential of Gardenia jasminoides leaves extract was evaluated by using various antioxidant assays. Methods: Various antioxidant assays such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, reducing power and total antioxidant capacity expressed as equivalent to ascorbic acid were employed. Moreover, phenolic compounds were detected by high-performance liquid chromatography (HPLC) coupled with diode-array detection. Results: The methanol extract showed significant free radical scavenging activities in DPPH radical scavenging antioxidant assays compared to the reference antioxidant ascorbic acid. Total antioxidant activity was increased in a dose dependent manner. The extract also showed strong reducing power. The total phenolic content was determined as 190.97 mg/g of gallic acid equivalent. HPLC coupled with diode-array detection was used to identify and quantify the phenolic compounds in the extracts. Gallic acid, (+)-catechin, rutin hydrate and quercetin have been identified in the plant extracts. Among the phenolic compounds, catechin and rutin hydrate are present predominantly in the extract. The accuracy and precision of the presented method were corroborated by low intra- and inter-day variations in quantitative results in leaves extract. Conclusion: These results suggest that phenolic compounds and flavonoids might contribute to high antioxidant activities of Gardenia jasminoides leaves. PMID:24754012

  2. Gardenia jasminoides Extract Attenuates the UVB-Induced Expressions of Cytokines in Keratinocytes and Indirectly Inhibits Matrix Metalloproteinase-1 Expression in Human Dermal Fibroblasts

    PubMed Central

    Seok, Jin Kyung; Suh, Hwa-Jin

    2014-01-01

    Ultraviolet radiation (UV) is a major cause of photoaging, which also involves inflammatory cytokines and matrix metalloproteinases (MMP). The present study was undertaken to examine the UVB-protecting effects of yellow-colored plant extracts in cell-based assays. HaCaT keratinocytes were exposed to UVB in the absence or presence of plant extracts, and resulting changes in cell viability and inflammatory cytokine expression were measured. Of the plant extracts tested, Gardenia jasminoides extract showed the lowest cytotoxicity and dose-dependently enhanced the viabilities of UVB-exposed cells. Gardenia jasminoides extract also attenuated the mRNA expressions of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in HaCaT cells stimulated by UVB. Conditioned medium from UVB-exposed HaCaT cells was observed to stimulate MMP-1 protein expression in human dermal fibroblasts, and this effect was much smaller for the conditioned medium of HaCaT cells exposed to UVB in the presence of Gardenia jasminoides extract. Gardenia jasminoides extract also exhibited antioxidative and antiapoptotic effects in HaCaT cells exposed to UVB. These results indicated that UVB-induced injury and inflammatory responses of skin cells can be attenuated by yellow-colored plant extracts, such as Gardenia jasminoides extract. PMID:24711853

  3. Monoamine Oxidase and Dopamine β-Hydroxylase Inhibitors from the Fruits of Gardenia jasminoides

    PubMed Central

    Kim, Ji Ho; Kim, Gun Hee; Hwang, Keum Hee

    2012-01-01

    This research was designed to determine what components of Gardenia jasminoides play a major role in inhibiting the enzymes related antidepressant activity of this plant. In our previous research, the ethyl acetate fraction of G. jasminosides fruits inhibited the activities of both monoamine oxidase-A (MAO-A) and monoamine oxidase-B (MAO-B), and oral administration of the ethanolic extract slightly increased serotonin concentrations in the brain tissues of rats and decreased MAO-B activity. In addition, we found through in vitro screening test that the ethyl acetate fraction showed modest inhibitory activity on dopamine-β hydroxylase (DBH). The bioassay-guided fractionation led to the isolation of five bio-active compounds, protocatechuic acid (1), geniposide (2), 6'-O-trans-p-coumaroylgeniposide (3), 3,5-d-ihydroxy-1,7-bis (4-hydroxyphenyl) heptanes (4), and ursolic acid (5), from the ethyl acetate fraction of G. jasminoides fruits. The isolated compounds showed different inhibitory potentials against MAO-A, -B, and DBH. Protocatechuic acid showed potent inhibition against MAO-B (IC50 300 μmol/L) and DBH (334 μmol/L), exhibiting weak MAO-A inhibition (2.41 mmol/L). Two iridoid glycosides, geniposide (223 μmol/L) and 6'-O-trans-p-coumaroylgeniposide (127μmol/L), were selective MAO-B inhibitor. Especially, 6'-O-trans-p-coumaroylgeniposide exhibited more selective MAO-B inhibition than deprenyl, well-known MAO-B inhibitor for the treatment of early-stage Parkinson’s disease. The inhibitory activity of 3,5-di-hydroxy-1,7-bis (4-hydroxyphenyl) heptane was strong for MAO-B (196 μmol/L), modest for MAO-A (400 μmol/L), and weak for DBH (941 μmol/L). Ursolic acid exhibited significant inhibition of DBH (214 μmol/L), weak inhibition of MAO-B (780 μmol/L), and no inhibition against MAO-A. Consequently, G. jasminoides fruits are considerable for development of biofunctional food materials for the combination treatment of depression and neurodegenerative disorders

  4. Gardenia jasminoides extracts and gallic acid inhibit lipopolysaccharide-induced inflammation by suppression of JNK2/1 signaling pathways in BV-2 cells

    PubMed Central

    Lin, Wen-Hung; Kuo, Heng-Hung; Ho, Li-Hsing; Tseng, Ming-Lang; Siao, An-Ci; Hung, Chang-Tsen; Jeng, Kee-Ching; Hou, Chien-Wei

    2015-01-01

    Objective(s): Gardenia jasminoides Ellis (GJ, Cape Jasmine Fruit, Zhi Zi) has been traditionally used for the treatment of infectious hepatitis, aphthous ulcer, and trauma; however, the direct evidence is lacking. Materials and Methods: We investigated the effect of the GJ extract (GJ) and gallic acid (GA) on lipopolysaccharide (LPS) induced inflammation of BV-2 microglial cells and acute liver injury in Sprague-Dawley (SD) rats. Results: Our results showed that the GJ extract and GA reduced LPS-induced nitric oxide (NO), interleukin (IL)-1, IL-6, reactive oxygen species (ROS), and prostaglandin (PGE2) production in BV-2 cells. The GJ extract and GA significantly decreased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in LPS-treated rats. Furthermore, the water extract, but not the ethanol extract, of the GJ dose-dependently inhibited LPS-induced JNK2/1 and slightly p38 mitogen-activated protein kinases (MAPK), and cyclooxygenase-2 (COX-2) expression in BV-2 cells. Conclusion: Taken together, these results indicate that the protective mechanism of the GJ extract involves an antioxidant effect and inhibition of JNK2/1 MAP kinase and COX-2 expressions in LPS-induced inflammation of BV-2 cells. PMID:26221479

  5. The methanol extract of Euonymus laxiflorus, Rubia lanceolata and Gardenia jasminoides inhibits xanthine oxidase and reduce serum uric acid level in rats.

    PubMed

    Liu, Li-Min; Cheng, Shu-Fen; Shieh, Po-Chuen; Lee, Jang-Chang; Chen, Jih-Jung; Ho, Chi-Tang; Kuo, Sheng-Chu; Kuo, Daih-Huang; Huang, Li-Jiau; Way, Tzong-Der

    2014-08-01

    Chinese herbal medicinal plants, Euonymus laxiflorus (EL), Rubia lanceolata (RL) and Gardenia jasminoides (GJ), have been used wildly to treat arthritis and gout in Taiwan for decades. To understand the beneficial effects of these three plants, their xanthine oxidase (XO) inhibitory activity in vitro and hypouricaemic activity in vivo were investigated. Our results suggested that methanol extracts were better than water extracts for inhibition of XO activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, except the water extract of GJ, which exhibited the strongest radical scavenging effect. In animal study, the serum urate level was significantly decreased after oral administration of higher dose (0.39g/kg) methanol extract of the mixture of three plants (ERG). In addition, methanol extract of ERG reduced the pain reaction time in the second phase of formalin induced pain. The results provide useful information on the pharmacological activities of these plants for the potential in treating hyperuricemia.

  6. Simultaneous determination of bioactive marker compounds from Gardeniae fructus by high performance liquid chromatography.

    PubMed

    Lee, Eun Ju; Hong, Jun Kee; Whang, Wan Kyunn

    2014-08-01

    Gardeniae fructus is one of the medicinal herbs that have been used in Far Eastern countries, such as Korea, China, and Japan. Gardeniae fructus is the dried ripe fruit of Gardenia jasminoides Ellis (Rubiaceae) and has been used as a yellow dye. It is widely used as traditional herbal medicine for reducing fever, cholagogue, diuretic and antiphlogistic effects. We established an analytical method that was useful to evaluate the quality control, and standardize quantification monitoring of 68 samples of Gardeniae fructus collected from Korea and China. While numerous previous studies have focused on the simultaneous analysis of geniposide, which constitutes the higher proportion of Gardeniae fructus, and crocin, which determines its color, no simultaneous analysis of gardenoside and geniposide, the major components of Gardeniae Fructus, has been performed. However, previously reported methods are not considered accurate enough because only geniposide or gardenoside was chosen to be the marker component for the quality control of Gardeniae fructus. Thus, we developed the method using simultaneous determination of four components including geniposide, gardenoside, geniposic acid and chlorogenic acid. Against this backdrop, this study aims to propose a new calculation for gardenoside and geniposide concentrations by analyzing their concentrations in Gardeniae fructus. PMID:24277694

  7. A strategy for identification and structural characterization of compounds from Gardenia jasminoides by integrating macroporous resin column chromatography and liquid chromatography-tandem mass spectrometry combined with ion-mobility spectrometry.

    PubMed

    Wang, Lu; Liu, Shu; Zhang, Xueju; Xing, Junpeng; Liu, Zhiqiang; Song, Fengrui

    2016-06-24

    In this paper, an analysis strategy integrating macroporous resin (AB-8) column chromatography and high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) combined with ion mobility spectrometry (IMS) was proposed and applied for identification and structural characterization of compounds from the fruits of Gardenia jasminoides. The extracts of G. jasminoides were separated by AB-8 resin column chromatography combined with reversed phase liquid chromatography (C18 column) and detected by electrospray ionization tandem mass spectrometry. Additionally, ion mobility spectrometry (IMS) was employed as a supplementary separation technique to discover previously undetected isomers from the fruits of G. jasminoides. A total of 71 compounds, including iridoids, flavonoids, triterpenes, monoterpenoids, carotenoids and phenolic acids were identified by the characteristic high resolution mass spectrometry and the ESI-MS/MS fragmentations. In conclusion, the IMS-MS technique achieved the separation of isomers in crocin-3 and crocin-4 according to their acquired mobility drift times differing from classical analysis by mass spectrometry. The proposed strategy can be used as a highly sensitive and efficient procedure for identification and separation isomeric components in extracts of herbal medicines.

  8. A strategy for identification and structural characterization of compounds from Gardenia jasminoides by integrating macroporous resin column chromatography and liquid chromatography-tandem mass spectrometry combined with ion-mobility spectrometry.

    PubMed

    Wang, Lu; Liu, Shu; Zhang, Xueju; Xing, Junpeng; Liu, Zhiqiang; Song, Fengrui

    2016-06-24

    In this paper, an analysis strategy integrating macroporous resin (AB-8) column chromatography and high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) combined with ion mobility spectrometry (IMS) was proposed and applied for identification and structural characterization of compounds from the fruits of Gardenia jasminoides. The extracts of G. jasminoides were separated by AB-8 resin column chromatography combined with reversed phase liquid chromatography (C18 column) and detected by electrospray ionization tandem mass spectrometry. Additionally, ion mobility spectrometry (IMS) was employed as a supplementary separation technique to discover previously undetected isomers from the fruits of G. jasminoides. A total of 71 compounds, including iridoids, flavonoids, triterpenes, monoterpenoids, carotenoids and phenolic acids were identified by the characteristic high resolution mass spectrometry and the ESI-MS/MS fragmentations. In conclusion, the IMS-MS technique achieved the separation of isomers in crocin-3 and crocin-4 according to their acquired mobility drift times differing from classical analysis by mass spectrometry. The proposed strategy can be used as a highly sensitive and efficient procedure for identification and separation isomeric components in extracts of herbal medicines. PMID:27208986

  9. Isolation and purification of six iridoid glycosides from gardenia jasminoides fruit by medium-pressure liquid chromatography combined with macroporous resin chromatography.

    PubMed

    Wang, Yun; Liu, Hui; Shen, Lifeng; Yao, Lan; Ma, Yinlian; Yu, Dingrong; Chen, Jianhong; Li, Puling; Chen, Ying; Zhang, Cun

    2015-12-01

    Gardeniae fructus is one of the most frequently used herbs in traditional Chinese medicine. In the present study, a process for the enrichment of six iridoid glycosides from Gardeniae fructus was developed using medium-pressure liquid chromatography combined with macroporous resin and reversed-phase chromatography. The purities of different fractions from Gardeniae fructus were assessed using quantitative high-performance liquid chromatography. After fractionation using HPD-100 column chromatography, a 30% ethanol fraction was selected based on high-performance liquid chromatography and liquid chromatography with mass spectrometry qualitative analysis to separate and purify. Based on the orientation analysis results, six compounds-deacetyl asperulosidic acid methyl ester, gardenoside, ixoroside, scandoside methyl ester, genipin-1-O-β-d-gentiobioside, and geniposide-were successfully isolated and purified in three to four combined steps from Gardeniae fructus. The purities of these compounds were found by high-performance liquid chromatography analysis to be 97.9, 98.1, 95.5, 96.3, 97.1, and 98.7%, respectively. Moreover, their structures were elucidated by NMR spectroscopy and liquid chromatography with tandem mass spectrometry. The separation process was highly efficient, rapid, and accurate, making it a potential approach for the large-scale production of iridoids in the laboratory and providing several marker compounds for quality control. This procedure may be meaningful for the purification of other natural products used in traditional Chinese medicine. PMID:26768549

  10. Isolation and purification of six iridoid glycosides from gardenia jasminoides fruit by medium-pressure liquid chromatography combined with macroporous resin chromatography.

    PubMed

    Wang, Yun; Liu, Hui; Shen, Lifeng; Yao, Lan; Ma, Yinlian; Yu, Dingrong; Chen, Jianhong; Li, Puling; Chen, Ying; Zhang, Cun

    2015-12-01

    Gardeniae fructus is one of the most frequently used herbs in traditional Chinese medicine. In the present study, a process for the enrichment of six iridoid glycosides from Gardeniae fructus was developed using medium-pressure liquid chromatography combined with macroporous resin and reversed-phase chromatography. The purities of different fractions from Gardeniae fructus were assessed using quantitative high-performance liquid chromatography. After fractionation using HPD-100 column chromatography, a 30% ethanol fraction was selected based on high-performance liquid chromatography and liquid chromatography with mass spectrometry qualitative analysis to separate and purify. Based on the orientation analysis results, six compounds-deacetyl asperulosidic acid methyl ester, gardenoside, ixoroside, scandoside methyl ester, genipin-1-O-β-d-gentiobioside, and geniposide-were successfully isolated and purified in three to four combined steps from Gardeniae fructus. The purities of these compounds were found by high-performance liquid chromatography analysis to be 97.9, 98.1, 95.5, 96.3, 97.1, and 98.7%, respectively. Moreover, their structures were elucidated by NMR spectroscopy and liquid chromatography with tandem mass spectrometry. The separation process was highly efficient, rapid, and accurate, making it a potential approach for the large-scale production of iridoids in the laboratory and providing several marker compounds for quality control. This procedure may be meaningful for the purification of other natural products used in traditional Chinese medicine.

  11. Quality control of Gardeniae Fructus by HPLC-PDA fingerprint coupled with chemometric methods.

    PubMed

    Yin, Fangzhou; Wu, Xiaoyan; Li, Lin; Chen, Yan; Lu, Tuling; Li, Weidong; Cai, Baochang; Yin, Wu

    2015-01-01

    The ripe fruits of Gardenia jasminoides Ellis have been used as traditional Chinese medicine to treat diseases for a long history. Lines of evidence demonstrate that multiple active constituents are responsible for the therapeutic effects of this herbal medicine. However, effective methods for quality control of this herbal medicine are still lacking. In this study, a high-performance liquid chromatography (HPLC) fingerprint analysis was performed on a SinoChrom ODS-BP C18 column (4.6 mm × 250 mm, 5 μm) at 30°C with mobile phase of aqueous solution with 0.1% formic acid and acetonitrile. On the basis of the chromatographic data from 32 batches samples, the HPLC fingerprint pattern containing 27 common peaks was obtained. Among these common peaks, seven peaks were identified by the electrospray ionization-mass spectrometry as geniposidic acid, genipin-1-β-gentiobioside, chlorogenic acid, geniposide, rutin, crocin-1 and crocin-2 and the contents of these seven compounds were simultaneously determined. Finally, chemometric methods including hierarchical clustering analysis and principal component analysis were successfully applied to differentiate the samples from six producing regions. In sum, the data, as described in this study, offer valuable information for the quality control and proper use of Gardeniae Fructus. PMID:26071608

  12. Instant and Persistent Antidepressant Response of Gardenia Yellow Pigment Is Associated with Acute Protein Synthesis and Delayed Upregulation of BDNF Expression in the Hippocampus.

    PubMed

    Wu, Ruyan; Tao, Weiwei; Zhang, Hailou; Xue, Wenda; Zou, Zhilu; Wu, Haoxin; Cai, Baochang; Doron, Ravid; Chen, Gang

    2016-08-17

    Gardenia yellow pigment (GYP) is a collection of compounds with shared structure of crocin, which confers antidepressant activity. GYP is remarkably enriched in Gardenia jasminoides Ellis, implicated in rapid antidepressant effects that are exerted through enhanced neuroplasticity. This study aims to investigate the rapid antidepressant-like activity of GYP and its underlying mechanism. After the optimal dose was determined, antidepressant responses in tail suspension test or forced swim test were monitored at 30 min, 1 day, 3 days, and 7 days post a single GYP administration. Rapid antidepressant potential was tested using learned helplessness paradigm. The expression of proteins involved in hippocampal neuroplasticity was determined. The effect of blockade of protein synthesis on GYP's antidepressant response was examined. Antidepressant response was detected at 30 min, and lasted for at least 3 days post a single administration of GYP. A single administration of GYP also reversed the deficits in learned helplessness test. Thirty minutes post GYP administration, ERK signaling was activated, and its downstream effector phosphorylated eukaryotic elongation factor 2 was inhibited, contributing to increased protein translation. Expression of synaptic proteins GluR1 and synapsin 1 was upregulated. Blockade of protein synthesis with anisomycin blunted the immediate antidepressant response of GYP. CREB signaling and BDNF expression were upregulated at 24 h, but not at 30 min. In conclusion, GYP-induced immediate antidepressant response was dependent on synthesis of proteins, including synaptic proteins. This was followed by enhanced expression of CREB and BDNF, which likely mediated the persistent antidepressant responses. PMID:27203575

  13. Quality control and producing areas differentiation of Gardeniae Fructus for eight bioactive constituents by HPLC-DAD-ESI/MS.

    PubMed

    Wu, Xiaoyan; Zhou, Yuan; Yin, Fangzhou; Mao, Chunqin; Li, Lin; Cai, Baochang; Lu, Tulin

    2014-03-15

    Gardeniae Fructus (G.Fructus), the fruit of Gardenia jasminoides Ellis (Rubiaceae), is a commonly used traditional Chinese medicine (TCM) that has been used for the treatment of hepatitis, jaundice, hypersonic, diabetes and hematuria. Numerous researches have demonstrated that the major active constituents in G.Fructus were responsible for the majority of medical effects of this fruit and their quantification were important for the quality control of G.Fructus. However, in the current quality control standard, only geniposide was used as characteristic marker of G.Fructus, which could not reflect the overall quality of this fruit. In order to identify more chemical makers for improving the quality control standard and evaluate producing areas differentiation of G.Fructus, in the present study, a novel and sensitive high-performance liquid chromatography-diode array detector coupled to an electrospray tandem mass spectrometer (HPLC-DAD-ESI/MS) was developed for the simultaneous determination of 8 major constituents, including geniposidic acid (1), chlorogenic acid (2), genipin-1-β-gentiobioside (3), geniposide (4), genipin (5), rutin (6), crocin-1 (7), crocin-2 (8) in G.Fructus. Moreover, chemometric analysis techniques with principal component constituent analysis (PCA) and cluster analysis (CA) involved were introduced in statistical analysis of 8 investigated constituents in the 34 batches samples to discriminate the samples from different producing areas. The results indicated that the contents of the 8 major bioactive constituents in G.Fructus varied significantly among different producing areas. From results of the loading plot from PCA analysis, genipin-1-β-gentiobioside may have more influence in discriminating the sample from different producing areas, and which was found to be the most abundant bioactive component besides geniposide in all the 34 batches samples, suggesting that it should be added as chemical marker for further investigation on the

  14. Quality control and producing areas differentiation of Gardeniae Fructus for eight bioactive constituents by HPLC-DAD-ESI/MS.

    PubMed

    Wu, Xiaoyan; Zhou, Yuan; Yin, Fangzhou; Mao, Chunqin; Li, Lin; Cai, Baochang; Lu, Tulin

    2014-03-15

    Gardeniae Fructus (G.Fructus), the fruit of Gardenia jasminoides Ellis (Rubiaceae), is a commonly used traditional Chinese medicine (TCM) that has been used for the treatment of hepatitis, jaundice, hypersonic, diabetes and hematuria. Numerous researches have demonstrated that the major active constituents in G.Fructus were responsible for the majority of medical effects of this fruit and their quantification were important for the quality control of G.Fructus. However, in the current quality control standard, only geniposide was used as characteristic marker of G.Fructus, which could not reflect the overall quality of this fruit. In order to identify more chemical makers for improving the quality control standard and evaluate producing areas differentiation of G.Fructus, in the present study, a novel and sensitive high-performance liquid chromatography-diode array detector coupled to an electrospray tandem mass spectrometer (HPLC-DAD-ESI/MS) was developed for the simultaneous determination of 8 major constituents, including geniposidic acid (1), chlorogenic acid (2), genipin-1-β-gentiobioside (3), geniposide (4), genipin (5), rutin (6), crocin-1 (7), crocin-2 (8) in G.Fructus. Moreover, chemometric analysis techniques with principal component constituent analysis (PCA) and cluster analysis (CA) involved were introduced in statistical analysis of 8 investigated constituents in the 34 batches samples to discriminate the samples from different producing areas. The results indicated that the contents of the 8 major bioactive constituents in G.Fructus varied significantly among different producing areas. From results of the loading plot from PCA analysis, genipin-1-β-gentiobioside may have more influence in discriminating the sample from different producing areas, and which was found to be the most abundant bioactive component besides geniposide in all the 34 batches samples, suggesting that it should be added as chemical marker for further investigation on the

  15. Preparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography.

    PubMed

    Kim, Chul Young; Kim, Jinwoong

    2007-01-01

    The iridoid glycoside, geniposide was purified by centrifugal partition chromatography (CPC) with a two-phase solvent system composed of ethyl acetate:isopropanol:water (3:2:5, v/v) from an 80% methanolic extract of fruits of Gardenia jasminoides. Preparative CPC yielded 56.2 mg of geniposide in a one-step separation of 500 mg of extract, with a purity of 95% as determined by HPLC. Isolated geniposide was identified from its 1H-NMR, 13C-NMR and MS spectra.

  16. Light harvesting over a wide range of wavelength using natural dyes of gardenia and cochineal for dye-sensitized solar cells

    NASA Astrophysics Data System (ADS)

    Park, Kyung-Hee; Kim, Tae-Young; Han, Shin; Ko, Hyun-Seok; Lee, Suk-Ho; Song, Yong-Min; Kim, Jung-Hun; Lee, Jae-Wook

    2014-07-01

    Two natural dyes extracted from gardenia yellow (Gardenia jasminoides) and cochineal (Dactylopius coccus) were used as sensitizers in the assembly of dye-sensitized solar cells (DSSCs) to harvest light over a wide range of wavelengths. The adsorption characteristics, electrochemical properties and photovoltaic efficiencies of the natural DSSCs were investigated. The adsorption kinetics data of the dyes were obtained in a small adsorption chamber and fitted with a pseudo-second-order model. The photovoltaic performance of a photo-electrode adsorbed with single-dye (gardenia or cochineal) or the mixture or successive adsorption of the two dyes, was evaluated from current-voltage measurements. The energy conversion efficiency of the TiO2 electrode with the successive adsorption of cochineal and gardenia dyes was 0.48%, which was enhanced compared to single-dye adsorption. Overall, a double layer of the two natural dyes as sensitizers was successfully formulated on the nanoporous TiO2 surface based on the differences in their adsorption affinities of gardenia and cochineal.

  17. Light harvesting over a wide range of wavelength using natural dyes of gardenia and cochineal for dye-sensitized solar cells.

    PubMed

    Park, Kyung-Hee; Kim, Tae-Young; Han, Shin; Ko, Hyun-Seok; Lee, Suk-Ho; Song, Yong-Min; Kim, Jung-Hun; Lee, Jae-Wook

    2014-07-15

    Two natural dyes extracted from gardenia yellow (Gardenia jasminoides) and cochineal (Dactylopius coccus) were used as sensitizers in the assembly of dye-sensitized solar cells (DSSCs) to harvest light over a wide range of wavelengths. The adsorption characteristics, electrochemical properties and photovoltaic efficiencies of the natural DSSCs were investigated. The adsorption kinetics data of the dyes were obtained in a small adsorption chamber and fitted with a pseudo-second-order model. The photovoltaic performance of a photo-electrode adsorbed with single-dye (gardenia or cochineal) or the mixture or successive adsorption of the two dyes, was evaluated from current-voltage measurements. The energy conversion efficiency of the TiO2 electrode with the successive adsorption of cochineal and gardenia dyes was 0.48%, which was enhanced compared to single-dye adsorption. Overall, a double layer of the two natural dyes as sensitizers was successfully formulated on the nanoporous TiO2 surface based on the differences in their adsorption affinities of gardenia and cochineal. PMID:24709352

  18. Havelock Ellis, eugenicist.

    PubMed

    Crozier, Ivan

    2008-06-01

    This article examines the contributions made towards eugenic thought in the late nineteenth and early twentieth centuries by Havelock Ellis (1859-1939). Ellis was a significant social reformer who worked on the problems of sexuality from a scientific-naturalist secular perspective. In the later phases of his work, after he had completed much of his writing on sexuality, Ellis concentrated on issues of feminism and eugenics--problems he thought were interlinked. In this paper, I integrate his ideas about these subjects, and consider the ways in which Ellis and other liberal social reformers created a 'eugenic subject' in order to frame their arguments about social problems. PMID:18534349

  19. Unconventional Therapist: Albert Ellis.

    ERIC Educational Resources Information Center

    Weinrach, Stephen G.

    1980-01-01

    Albert Ellis is one of counseling's most prolific authors, mostly on the topic of Rational Emotive Therapy. He has been a moving force in the cognitive behavior movement. In this interview Ellis discusses his theory and its application, and aspects of his personal and family life. (Author)

  20. Dr. Ellis, Please Stand Up

    ERIC Educational Resources Information Center

    Meichenbaum, Robert

    1977-01-01

    The author speaks to Albert Ellis' article and considers the article too naive and simplistic. He particularly questions Ellis' final conclusion that RET is based on a strong empirical foundation. (HMV)

  1. Commentary on Albert Ellis' Article

    ERIC Educational Resources Information Center

    Kleiner, Frederic B.

    1977-01-01

    In a response to Albert Ellis' feature article, the author agrees with Ellis but feels that less time should be spent proving which counseling method is better than the next, and more time spent in comparative research as per clients' gains. (HMV)

  2. Ellis van Creveld syndome.

    PubMed

    Ghanekar, Jaishree; Sangrampurkar, Sujata; Hulinaykar, Raman; Ahmer, Tariq

    2009-07-01

    Ellis-van Creveld syndrome (EVC) or chondroectodermal dysplasia is a rare autosomal recessive disorder. It is a tetrad of chondrodysplasia, ectodermal dysplasia, polydactyly, and congenital heart disease. In several case reports, dysplasia involving other organs has also been identified. The exact prevalence is unknown, but the syndrome seems more common among the Amish community. Many Indian cases have also been reported. This report describes a classical case of EVC syndrome in a 22 year old woman of Indian origin born of a consanginous marriage. The patient had chondrodysplasia of tubular bones resulting in disproportionate dwarfism, postaxial polydactyly, severely dystrophic nails, partially absent teeth, pectus excavatum with narrow chest, knock knees and AV canal defect. PMID:20329417

  3. Ellis-van Creveld syndrome

    MedlinePlus

    Chondroectodermal dysplasia; EVC ... 1 of 2 Ellis-van Creveld syndrome genes ( EVC and EVC2 ). These genes are positioned next to ... performed for mutations in 1 of the 2 EVC genes Skeletal x-ray Ultrasound Urinalysis

  4. [Analysis of volatile ingredients in Gardeniae Fructus and its processed products by GC-MS].

    PubMed

    Liu, Hui; Yao, Lan; Chen, Jian-hong; Gu, Xue-zhu; Ma, Yin-lian; Chen, Ying; Li, Pu-ling; Zhang, Cun

    2015-05-01

    Gardeniae Fructus contains volatile ingredients, however, the species and proportions in different processed products of Gardeniae Fructus are different. In this experiment, volatile ingredients were separated by steam distillation with content of 1.2, 1.0, 0.9, 0.7 µL · g(-1) in Gardeniae Fructus, fried Gardeniae Fructus, stir-baked Gardeniae Fructus, Gardeniae Fructus fried into carbon respectively. One hundred and twenty-four kinds of volatile components were identified by GC-MS. Fifty-three kinds of volatile ingredients consisted in Gardeniae Fructus accounting for 93.85%, 54 kinds in fried Cardeniae Fructus accounting for 92.01%, 32 kinds in stir-baked Cardeniae Fructus accounting for 91.59% and 43 kinds in Gardeniae Fructus fried into carbon accounting for 90.81%. In this paper, analysis of Gardeniae Fructus by GC-MS provides a scientific basis for elucidating the mechanism of different processed products. PMID:26323138

  5. Triterpenes from the exudate of Gardenia urvillei.

    PubMed

    Mai, Hoang Linh; Grellier, Philippe; Prost, Elise; Lemoine, Pascale; Poullain, Cyril; Dumontet, Vincent; Deguin, Brigitte; Vo, Thi Bach Hue; Michel, Sylvie; Grougnet, Raphaël

    2016-02-01

    A cycloartane gardurvilleic acid, three 3,4-seco-cycloartanes securvienol, secodienurvilleic acid, securvitriol, a 3,4;9,10-seco-cycloartane gardheptlactone, two dammaranes urvilone, urvilol, along with eight known cycloartanes and 3,4-seco-cycloartanes and four known dammaranes have been isolated from the bud exudate of Gardenia urvillei, an endemic tree to the New Caledonian dry forest. Two other dammarane derivatives have been obtained by semisynthesis. The structures of the original compounds were determined by spectroscopic methods and chemical correlations. In association with previously published data, the description of oxidized side-chains in position 17 are now available for two couples of diastereoisomers. Evaluation of anti-parasite activity and cytotoxicity has shown noticeable results for some of the isolated triterpenes. PMID:26608669

  6. [Analysis on formula raw materials application of health food containing Gardeniae fructus].

    PubMed

    Zhang, Yi-guang; Tang, Shi-huan; Jia, Qiang; Meng, Fan-yun

    2014-11-01

    In this article we built formula database of health food containing Gardeniae Fructus with Traditional Chinese Medicine Inheritance Support System (V2.0). And on this basis, use data mining method such as association rules of the software, to analyze commonly used formula raw materials or materials combination of formula containing Gardeniae Fructus and raw material application having assisted function formula to protect chemical liver injury. The result shows that of the 71 health food formulas containing Gardeniae Fructus, most used materials are Gardeniae Fructus, Lycii Fructus, Angelica Sinensis Radix, Poria and so on. Commonly used materials combination mostly are Gardeniae Fructus and Lycii Fructus, Gardeniae Fructus and Angelica Sinensis Radix, Gardeniae Fructus and Poria, Gardeniae Fructus and Paeonia. There are nearly 18 healthcare functions of the health food containing Gardeniae Fructus, and most of these are assisted functions to protect chemical liver injury, and then immune modulating function. Of 23 formulas containing Gardeniae Fructus having assisted function formula to protect chemical liver injury, Gardeniae Fructus usually combined with traditional Chinese medicine which nourishs blood and liver such as Pueraria, Lycii Fructus, Hawthorn, Paeonia and Turnjujube. Analyzing formula raw materials application of health food containing Gardeniae Fructus contributes a lot to the further development and utilization. PMID:25850287

  7. Ellis-van Creveld syndrome

    PubMed Central

    Sasalawad, Shilpa S; Hugar, Shivayogi M; Poonacha, K S; Mallikarjuna, Rachappa

    2013-01-01

    Ellis-van Creveld (EvC) syndrome or chondroectodermal dysplasia is an autosomal recessive disorder with characteristic clinical manifestations. The four principal characteristics are chondrodysplasia, polydactyly, ectodermal dysplasia and congenital heart defects. Its incidence in the general population is low. The oral manifestations of EvC syndrome include both soft tissues and teeth, but the dental literature on the subject is scarce. The present case describes the constant and variable oral findings in these patients, which can be diagnosed at any age, even during pregnancy. The presence of a variety of oral manifestations, such as fusion of upper lip to the gingival margin, presence of multiple frenula, abnormally shaped and microdontic teeth and congenitally missing teeth requires multidisciplinary dental treatment, with consideration for the high incidence of cardiac defects in these patients. PMID:23843404

  8. Albert Ellis: An Efficient and Passionate Life.

    ERIC Educational Resources Information Center

    Dryden, Windy

    1989-01-01

    Presents interview of psychotherapist Albert Ellis who discusses his early days, the women in his life, and his personal characteristics and offers personal reflections on his professional career. (Author)

  9. Al Ellis: Up Close and Personal

    ERIC Educational Resources Information Center

    Eckstein, Daniel; Ellis, Debbie Joffe

    2011-01-01

    The purpose of the following article is to share some of the personal relationship comments and to provide a tribute to the significant 93 years that Albert Ellis lived and also make major contributions to the counseling profession.

  10. 8. NORTH ELLIS WORKS LOOKING SOUTHWEST. BOTTLING HOUSE, RIGHT, FIVE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. NORTH ELLIS WORKS LOOKING SOUTHWEST. BOTTLING HOUSE, RIGHT, FIVE CHAMBER CONDENSER, AND BURIED NORTH ELLIS THREE PIPE INCLINED RETORT, BACKGROUND LEFT. - Mariscal Quicksilver Mine & Reduction Works, Terlingua, Brewster County, TX

  11. Ellis-van Creveld syndrome.

    PubMed

    Baujat, Geneviève; Le Merrer, Martine

    2007-06-04

    Ellis-van Creveld syndrome (EVC) is a chondral and ectodermal dysplasia characterized by short ribs, polydactyly, growth retardation, and ectodermal and heart defects. It is a rare disease with approximately 150 cases reported worldwide. The exact prevalence is unknown, but the syndrome seems more common among the Amish community. Prenatal abnormalities (that may be detected by ultrasound examination) include narrow thorax, shortening of long bones, hexadactyly and cardiac defects. After birth, cardinal features are short stature, short ribs, polydactyly, and dysplastic fingernails and teeth. Heart defects, especially abnormalities of atrial septation, occur in about 60% of cases. Cognitive and motor development is normal. This rare condition is inherited as an autosomal recessive trait with variable expression. Mutations of the EVC1 and EVC2 genes, located in a head to head configuration on chromosome 4p16, have been identified as causative. EVC belongs to the short rib-polydactyly group (SRP) and these SRPs, especially type III (Verma-Naumoff syndrome), are discussed in the prenatal differential diagnosis. Postnatally, the essential differential diagnoses include Jeune dystrophy, McKusick-Kaufman syndrome and Weyers syndrome. The management of EVC is multidisciplinary. Management during the neonatal period is mostly symptomatic, involving treatment of the respiratory distress due to narrow chest and heart failure. Orthopedic follow-up is required to manage the bones deformities. Professional dental care should be considered for management of the oral manifestations. Prognosis is linked to the respiratory difficulties in the first months of life due to thoracic narrowness and possible heart defects. Prognosis of the final body height is difficult to predict.

  12. Ellis-van Creveld syndrome: its history.

    PubMed

    Muensterer, Oliver J; Berdon, Walter; McManus, Chris; Oestreich, Alan; Lachman, Ralph S; Cohen, M Michael; Done, Stephen

    2013-08-01

    The story of Ellis-van Creveld syndrome is one of serendipity. By chance, Simon van Creveld and Richard Ellis purportedly met on a train and combined their independently encountered patients with short stature, dental anomalies and polydactyly into one landmark publication in 1940. They included a patient used in work published previously by Rustin McIntosh without naming McIntosh as a coauthor. This patient was followed radiologically by Caffey for nearly two decades. In 1964, Victor McKusick felt compelled to investigate a brief report in an obscure pharmaceutical journal on an unusual geographic cluster of short-statured Amish patients in Pennsylvania. This review highlights the lives of the individuals involved in the discovery of Ellis-van Creveld syndrome in their historic context. PMID:23754541

  13. John A. Scigliano Interviews Allan B. Ellis.

    ERIC Educational Resources Information Center

    Scigliano, John A.

    2000-01-01

    This interview with Allan Ellis focuses on a history of computer applications in education. Highlights include work at the Harvard Graduate School of Education; the New England Education Data System; and efforts to create a computer-based distance learning and development program called ISVD (Information System for Vocational Decisions). (LRW)

  14. Ellie Mannette: Master of the Steel Drum.

    ERIC Educational Resources Information Center

    Svaline, J. Marc

    2001-01-01

    Presents an interview with Elliot ("Ellie") Mannette who has played a major role in the development and application of steel drums. States that he has spent most of his life designing and teaching the steel drums. Covers interview topics and background information on Mannette. (CMK)

  15. Your Biggest Game: Interview with Dave Ellis.

    ERIC Educational Resources Information Center

    Sparks, Dennis

    2001-01-01

    Interviews Dave Ellis, president of the Brande Foundation, who promotes life coaching to help leaders become more creative and effective by making them happier, more satisfied human beings. It provides an opportunity for people to look at all areas of their lives, determine what they want in these areas, and have the coach help them develop…

  16. Gravitational Microlensing by the Ellis Wormhole

    NASA Astrophysics Data System (ADS)

    Abe, F.

    2010-12-01

    A method to calculate light curves of the gravitational microlensing of the Ellis wormhole is derived in the weak-field limit. In this limit, lensing by the wormhole produces one image outside the Einstein ring and another image inside. The weak-field hypothesis is a good approximation in Galactic lensing if the throat radius is less than 1011 km. The light curves calculated have gutters of approximately 4% immediately outside the Einstein ring crossing times. The magnification of the Ellis wormhole lensing is generally less than that of Schwarzschild lensing. The optical depths and event rates are calculated for the Galactic bulge and Large Magellanic Cloud fields according to bound and unbound hypotheses. If the wormholes have throat radii between 100 and 107 km, are bound to the galaxy, and have a number density that is approximately that of ordinary stars, detection can be achieved by reanalyzing past data. If the wormholes are unbound, detection using past data is impossible.

  17. Albert Ellis's Theoretical Ark: Reactions of a Reader.

    ERIC Educational Resources Information Center

    Ginter, Earl J.

    1997-01-01

    Provides a brief reaction to Albert Ellis's "Postmodern Ethics for Active-Directive Counseling and Psychotherapy," which appears in this issue. Offers a condensed review of what has led up to Ellis's article. Pays special attention to the phrase "and/also" in relation to its implication for theory building and practice. (RJM)

  18. Second Language Acquisition Research: A Response to Rod Ellis.

    ERIC Educational Resources Information Center

    Hopkins, Diana; Nettle, Mark

    1994-01-01

    Two practicing English-as-a-Foreign-Language (EFL teachers respond to Rod Ellis' January 1993 article in "ELT Journal," which discussed importance of grammar instruction in EFL classrooms. Argues some of Ellis' assumptions about current classroom practices are inaccurate and a number of his "alternative" approaches to teaching grammar, such as…

  19. John Tracy Ellis and the Figure of the Catholic Intellectual

    ERIC Educational Resources Information Center

    Conniff, Brian

    2006-01-01

    Fifty years after John Tracy Ellis challenged the quality and character of Catholic intellectual life, much work remains to be done. This essay explores Ellis's original assertions and places them in an overarching historical context that involves Flannery O'Connor and Thomas Merton.

  20. Reason and emotion in psychotherapy: Albert Ellis.

    PubMed

    Dryden, W; Bond, F W

    1994-07-01

    To summarise then, in 1962 RET displayed important features still current. These include the interrelatedness of cognitive, emotive and behavioural processes, the important role that cognition plays in psychological problems, its humanistic view of the self, and the futility and dangers of self-rating. The emphasis on perpetuation rather than acquisition processes of emotional disturbance holds good now as it did then, and the core view of therapeutic change is essentially the same now as it was in 1962, despite further, more recent elaborations. Significant change has occurred in RET since 1962 that updates several of Ellis' original ideas. These include the distinction between interpretations (or inferences) and evaluations, the primary of musts in accounting for psychological disturbance, the clear distinction between healthy and unhealthy negative emotions and the greater role according to force and energy in the change process. In addition, a greater emphasis is placed on biological aspects of emotional disturbance now than 30 years ago. Finally, a greater range of cognitive, imaginal, emotive and behavioural methods are found in current RET literature than in Reason and Emotion in Psychotherapy, where Ellis restricts himself to illustrating a few cognitive and behavioural techniques. RET, then, has grown and developed over the past 30 years. In large part, this reflects the theory's flexibility and the competent people who have worked to make RET one of the most viable and widely used cognitive-behaviour therapies.

  1. GRAVITATIONAL MICROLENSING BY THE ELLIS WORMHOLE

    SciTech Connect

    Abe, F.

    2010-12-10

    A method to calculate light curves of the gravitational microlensing of the Ellis wormhole is derived in the weak-field limit. In this limit, lensing by the wormhole produces one image outside the Einstein ring and another image inside. The weak-field hypothesis is a good approximation in Galactic lensing if the throat radius is less than 10{sup 11} km. The light curves calculated have gutters of approximately 4% immediately outside the Einstein ring crossing times. The magnification of the Ellis wormhole lensing is generally less than that of Schwarzschild lensing. The optical depths and event rates are calculated for the Galactic bulge and Large Magellanic Cloud fields according to bound and unbound hypotheses. If the wormholes have throat radii between 100 and 10{sup 7} km, are bound to the galaxy, and have a number density that is approximately that of ordinary stars, detection can be achieved by reanalyzing past data. If the wormholes are unbound, detection using past data is impossible.

  2. Ellis-van Creveld syndrome and dyserythropoiesis.

    PubMed

    Scurlock, Deven; Ostler, Daniel; Nguyen, Andy; Wahed, Amer

    2005-05-01

    Ellis-van Creveld (EVC) syndrome or chondroectodermal dysplasia is a rare autosomal recessive disorder characterized by a variable spectrum of clinical findings. Classical EVC syndrome comprises a tetrad of clinical manifestations of chondrodystrophy, polydactyly, ectodermal dysplasia, and cardiac defects. In several case reports, dysplasia involving other organs has also been identified. Hematologic abnormalities have been rarely reported in patients with EVC syndrome. Here, we report a case of a 3-year-old Hispanic boy with EVC syndrome and marked dyserythropoiesis. The dyserythropoiesis may be part of an isolated myelodysplastic change or a primary myelodysplastic syndrome and likely represents an unusual EVC syndrome association. To our knowledge, this association has not been previously reported.

  3. Genetics Home Reference: Ellis-van Creveld syndrome

    MedlinePlus

    ... bone growth that results in very short stature (dwarfism). People with this condition have particularly short forearms ... Ellis-van Creveld Syndrome Encyclopedia: Polydactyly Health Topic: Dwarfism Genetic and Rare Diseases Information Center (1 link) ...

  4. 75. SACRED HEART SCHOOL, 1324 ELLIS STREET SOUTH (REAR ELEVATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    75. SACRED HEART SCHOOL, 1324 ELLIS STREET SOUTH (REAR ELEVATION FROM GREENE STREET 56/61A - Greene Street Historic District, Greene Street, Gordon Highway to Augusta Canal Bridge, Augusta, Richmond County, GA

  5. [Process of iridoids in gardenia and its effect on relevant enzymes in vivo].

    PubMed

    Zhang, Yan; Zhu, Huaxu; Guo, Liwei

    2012-02-01

    To introduces the body process of iridoid in gardenia and effect of biological activity of enzymes systematically and discusses the mechanism of these compounds on the basis of the domestic and foreign recent literatures. It also provides a literature basis for the instruction of rational clinical prescription, reform of dosage forms, and development and utilization.

  6. 33 CFR 147.1104 - Platform ELLEN & ELLY safety zone.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Platform ELLEN & ELLY safety zone... Ellen, 33°-34′-57″ N, 118°-07′-42″ W; and Platform Elly, 33°-35′-00″ N, 118°-07′-40″ W. (b) Regulations... serving either structure, (2) a vessel under 100 feet in length overall not engaged in towing, or (3)...

  7. 33 CFR 147.1104 - Platform ELLEN & ELLY safety zone.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Platform ELLEN & ELLY safety zone... Ellen, 33°-34′-57″ N, 118°-07′-42″ W; and Platform Elly, 33°-35′-00″ N, 118°-07′-40″ W. (b) Regulations... serving either structure, (2) a vessel under 100 feet in length overall not engaged in towing, or (3)...

  8. 33 CFR 147.1104 - Platform ELLEN & ELLY safety zone.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Platform ELLEN & ELLY safety zone... Ellen, 33°-34′-57″ N, 118°-07′-42″ W; and Platform Elly, 33°-35′-00″ N, 118°-07′-40″ W. (b) Regulations... serving either structure, (2) a vessel under 100 feet in length overall not engaged in towing, or (3)...

  9. 33 CFR 147.1104 - Platform ELLEN & ELLY safety zone.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Platform ELLEN & ELLY safety zone... Ellen, 33°-34′-57″ N, 118°-07′-42″ W; and Platform Elly, 33°-35′-00″ N, 118°-07′-40″ W. (b) Regulations... serving either structure, (2) a vessel under 100 feet in length overall not engaged in towing, or (3)...

  10. 33 CFR 147.1104 - Platform ELLEN & ELLY safety zone.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Platform ELLEN & ELLY safety zone... Ellen, 33°-34′-57″ N, 118°-07′-42″ W; and Platform Elly, 33°-35′-00″ N, 118°-07′-40″ W. (b) Regulations... serving either structure, (2) a vessel under 100 feet in length overall not engaged in towing, or (3)...

  11. Photovoltaic performance of TiO2 electrode adsorbed with gardenia yellow purified by nonionic polymeric sorbent in dye-sensitized solar cells.

    PubMed

    Kwon, Oh Oun; Kim, Eui Jin; Lee, Jae Hyeok; Kim, Tae Young; Park, Kyung Hee; Kim, Sang Yook; Suh, Hwa Jin; Lee, Hyo Jung; Lee, Jae Wook

    2015-02-01

    To improve the photovoltaic conversion efficiency in dye-sensitized solar cells (DSSCs), TiO2 electrode adsorbed with gardenia yellow purified by nonionic polymeric sorbent was successfully formulated on nanoporous TiO2 surface. Adsorption and desorption properties of crude gardenia yellow solution on a macroporous resin, XAD-1600, were investigated to purify gardenia yellow because of its strong adsorption and desorption abilities as well as high selectivity. To this end, adsorption equilibrium and kinetic data were measured and fitted using adsorption isotherms and kinetic models. Adsorption and desorption breakthrough curves in a column packed with XAD-1600 resin was obtained to optimize the separation process of gardenia yellow. The photovoltaic performance of the photo-electrode adsorbed with the crude and purified gardenia yellow in DSSCs was compared from current-voltage measurements. The results showed that the photovoltaic conversion efficiency was highly dependent on how to separate and purify gardenia yellow as a photosensitizer.

  12. Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice

    PubMed Central

    Chen, Ping; Chen, Yang; Wang, Yarong; Cai, Shining; Deng, Liang; Liu, Jia; Zhang, Hao

    2016-01-01

    Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with CCl4. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in CCl4-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in CCl4-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on CCl4-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis. PMID:26902084

  13. Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice.

    PubMed

    Chen, Ping; Chen, Yang; Wang, Yarong; Cai, Shining; Deng, Liang; Liu, Jia; Zhang, Hao

    2016-03-01

    Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with CCl4. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantlylowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in CCl4-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in CCl4-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficialeffects on CCl4-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacyof Gardenia jasminoides Ellis. PMID:26902084

  14. Semen Ziziphi Spinosae and Fructus Gardeniae extracts synergistically improve learning and memory of a mouse model.

    PubMed

    Li, Baoli; Fu, Zhaoying; Hu, Rui; Chen, Yahui; Zhang, Zhengxiang

    2013-03-01

    Semen Ziziphi Spinosae (SZS) and Fructus Gardeniae (FG) are two herbs commonly used in traditional Chinese medicine. Previous studies have suggested that Fructus Gardeniae as well as Semen Ziziphi Spinosae are able to regulate the function of the central nervous system. However, their effect on learning and memory has yet to be elucidated. In this study, we examined the effect of SZS and FG on the learning and memory of mice using the methods of step-through and -down passive avoidance tasks and Morris water maze tasks. The results showed that SZS and FG extracts have certain effects on improving the performance of the learning and memory-impaired mouse model. Of note, compound extracts of SZS and FG have a synergistic effect on the learning and memory of mice. PMID:24648929

  15. Semen Ziziphi Spinosae and Fructus Gardeniae extracts synergistically improve learning and memory of a mouse model

    PubMed Central

    LI, BAOLI; FU, ZHAOYING; HU, RUI; CHEN, YAHUI; ZHANG, ZHENGXIANG

    2013-01-01

    Semen Ziziphi Spinosae (SZS) and Fructus Gardeniae (FG) are two herbs commonly used in traditional Chinese medicine. Previous studies have suggested that Fructus Gardeniae as well as Semen Ziziphi Spinosae are able to regulate the function of the central nervous system. However, their effect on learning and memory has yet to be elucidated. In this study, we examined the effect of SZS and FG on the learning and memory of mice using the methods of step-through and -down passive avoidance tasks and Morris water maze tasks. The results showed that SZS and FG extracts have certain effects on improving the performance of the learning and memory-impaired mouse model. Of note, compound extracts of SZS and FG have a synergistic effect on the learning and memory of mice. PMID:24648929

  16. Stir-baked Fructus gardeniae (L.) extracts inhibit matrix metalloproteinases and alter cell morphology.

    PubMed

    Yang, Jin-gang; Shen, Ye-hua; Hong, Yuan; Jin, Feng-hai; Zhao, Shu-hua; Wang, Ming-cui; Shi, Xiu-juan; Fang, Xue-xun

    2008-05-01

    Matrix metalloproteinases (MMPs) play vital roles in many pathological conditions, including cancer, cardiovascular disease, arthritis and inflammation. Modulating MMP activity may therefore be a useful therapeutic approach in treating these diseases. Qing-Kai-Ling is a popular Chinese anti-inflammatory formulation used to treat symptoms such as rheumatoid arthritis, acute hypertensive cerebral hemorrhage, hepatitis and upper respiratory tract infection. In this paper, we report that one of the components of Qing-Kai-Ling, Fructus gardeniae, strongly inhibits MMP activity. The IC50 values for the primary herbal extract and water extract against MMP-16 were 32 and 27 microg/ml, respectively. In addition, we show that the herbal extracts influence HT1080 human fibrosarcoma cell growth and morphology. These data may provide molecular mechanisms for the therapeutic effects of Qing-Kai-Ling and herbal medicinal Fructus gardeniae.

  17. The action potential of Dionaea muscipula Ellis.

    PubMed

    Hodick, D; Sievers, A

    1988-04-01

    The intention of this investigation was to acquire more concise information about the nature of the action potential of Dionaea muscipula Ellis and the different types of cells generating and conducting it. It is shown by microelectrode measurements that, besides the sensory cells, all the major tissues of the trap lobes are excitable, firing action potentials with pronounced after-hyperpolarizations. The action potentials are strictly dependent on Ca(2+). Their peak depolarizations are shifted 25-27 mV in a positive direction after a tenfold increase in external Ca(2+) concentration. Perfusions with 1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) or 1 mM LaCl3 completely inhibit excitability. Magnesium ions only slightly affect the peak depolarizations but considerably prolong action potentials. Sodium azide and 2,4-dinitrophenol also abolish excitation, probably by reducing the intracellular ATP concentration. Furthermore, it is tested whether the sensory cells can be distinguished from the other cells of the trap by their electrical behaviour. The resting potentials of sensory cells (-161±7 mV) and mesophyll cells (-155±8 mV) are of the same magnitude. Changes in external ion concentrations affect resting and action potentials in both cell types in a similar way. Additional freeze-fracture studies of both cell types reveal similar numbers and distributions of intramembrane particles on the fracture faces of the plasma membrane, which is most likely the mechanosensor. These findings stress the view that the high mechanosensitivity of the sensory hair results from its anatomy and not from a specialized perception mechanism. It is proposed that trap closure is triggered by a rise in the cytoplasmic concentration of Ca(2+) or a Ca(2+)-activated regulatory complex, which must exceed a threshold concentration. Since the Ca(2+) influx during a single action potential does not suffice to reach this threshold, at least two stimulations

  18. An Information Theory Analysis of Interviews by Ellis and Rogers.

    ERIC Educational Resources Information Center

    Lichtenberg, James W.; Tyndall, Larry W.

    Research on communication in counseling has identified/defined counselor communication behaviors which may affect client response and change. To further understand the pattern of interaction between counselor and client, transcripts of four initial interviews by Albert Ellis and two by Carl Rogers were analyzed for the pattern of responding within…

  19. Let's Nuke the Transpersonalists: A Response to Albert Ellis.

    ERIC Educational Resources Information Center

    Wilber, Ken

    1989-01-01

    Responds to Albert Ellis' 1986 article which proposed to use rational-emotive therapy (RET) to save the world from religious and psychological fanatics and nuclear war. Attempts to provide a more balanced view of religion, RET, non-RET therapies, and the role of psychology in averting nuclear war. (Author/ABL)

  20. Physical Illness and Subscription to Ellis' Irrational Beliefs.

    ERIC Educational Resources Information Center

    Johnson, Diana DeVore; Lichtenberg, James W.

    Research has shown a generally positive correlation between a person's propensity for illness and certain psychological variables. To investigate the relationship between an individual's age, sex, and degree of subscription to each of Albert Ellis' 10 irrational beliefs and their frequency and type of illness, 122 adults completed the Irrational…

  1. Evolving Science in Adolescence: Comment on Ellis et al. (2012)

    ERIC Educational Resources Information Center

    Dodge, Kenneth A.; Albert, Dustin

    2012-01-01

    Ellis et al. (2012) bring an evolutionary perspective to bear on adolescent risky behavioral development, clinical practice, and public policy. The authors offer important insights that (a) some risky behaviors may be adaptive for the individual and the species by being hard-wired due to fitness benefits and (b) interventions might be more…

  2. Affect, Albert Ellis, and Rational-Emotive Therapy.

    ERIC Educational Resources Information Center

    Morris, G. Barry

    1989-01-01

    Contends affect is integral component of Rational Emotive Therapy model. Reviews affective aspect of the model in terms of theoretical constructs and therapeutic techniques. Makes references to author-observed interactions of Albert Ellis and describes his life-style to permit inferences regarding the role of affect. Includes commentary by Ellis…

  3. Rational Emotive Behavior Therapy after Ellis: Predictions for the Future.

    ERIC Educational Resources Information Center

    Weinrach, Stephen G.; Ellis, Albert; DiGiuseppe, Raymond; Bernard, Michael E.; Dryden, Windy; Kassinove, Howard; Morris, G. Barry; Vernon, Ann; Wolfe, Janet

    1995-01-01

    Nine members of the institute for Rational-Emotive Therapy's (REBT) International Training Standards and Review Committee predicted the status of REBT 25 to 50 years after the death of Albert Ellis. Will REBT continue to exist in its own right or be incorporated into newer forms of cognitive behavior therapy? (EMK)

  4. Reconstructing Social Constructionism: A Response to Albert Ellis.

    ERIC Educational Resources Information Center

    Guterman, Jeffrey T.

    1996-01-01

    Offers a response to Albert Ellis' critique of social constructionist models in mental health counseling. Focuses on views of reality and on the distinctions between content and process, and examines formal and informal content. Likewise clarifies viewpoints on diagnosis and the client/counselor relationship. (EMK)

  5. Albert Ellis Revisited: Vague, General or Mild Religion.

    ERIC Educational Resources Information Center

    Floyd, William A.

    1984-01-01

    Discusses the writings of Albert Ellis dealing with religion and psychotherapy. Advocates a liberal form of theism in which (1) the use of symbolism and ritual are stressed; (2) faith is taken seriously but not as history or science; and (3) the importance of theology is affirmed. (JAC)

  6. Must the Rational Emotive Therapist Be like Albert Ellis?

    ERIC Educational Resources Information Center

    Johnson, Norbert

    1980-01-01

    Some counselors may avoid using Rational Emotive Therapy (RET) because they feel they cannot deal with clients in the active-directive manner of Albert Ellis, originator of RET. Some of the ways in which less actively directive counselors may use RET techniques are discussed. (Author)

  7. An Empirical Evaluation of Ellis' Rational Emotive Theory.

    ERIC Educational Resources Information Center

    Wright, Loyd S.; Couch, R. David

    Ellis' rational emotive theory is based on the assumption that people are born with a potential for both rational and irrational thinking. To analyze the relationship of irrational beliefs to sensitivity, depression, submission, anxiety, and neuroticism, an Irrationality Scale, containing eight irrational beliefs, was constructed from a review of…

  8. Ellis Island and the Peopling of America: The Official Guide.

    ERIC Educational Resources Information Center

    Yans-McLaughlin, Virginia; Lightman, Marjorie

    This guide book helps to place the Ellis Island Museum (New York) and site within the context of the worldwide, continuing history of immigration. The guide views immigration as a continuing social process that brings people from one part of the world to another where they contribute the richness of their labor to their newly adopted home. Ethnic…

  9. Counselors' Evaluation of Rogers-Perls-Ellis's Relationship Skills

    ERIC Educational Resources Information Center

    Woodward, Wallace S.; And Others

    1975-01-01

    Participants (12 employment counselors and 10 counselor supervisors) attending a three-week workshop on enhancing relationship skills, evaluated the Rogers, Perls, Ellis film, Three Approaches to Psychotherapy, on 15 skills. Results indicate there was general agreement between the counselors and the supervisors when judging levels of therapist…

  10. Preparative separation of crocins and geniposide simultaneously from gardenia fruits using macroporous resin and reversed-phase chromatography.

    PubMed

    Feng, Jianyong; He, Xihui; Zhou, Sheng; Peng, Fang; Liu, Jiangyun; Hao, Lili; Li, Heran; Ao, Guizhen; Yang, Shilin

    2014-02-01

    Gardenia fruits contain valuable natural food colorants including crocins (gardenia yellow) and geniposide. In this study, a process for the enrichment of crocins and geniposide simultaneously from gardenia fruits was developed using macroporous resin and RP chromatography. The performance of eight different types of macroporous resins was evaluated. Static absorption/desorption experiments revealed that LX60 possessed optimal separating capacity. Further dynamic absorption/desorption experiments on LX60 columns were conducted to obtain the optimal parameters. After one run treatment with LX60, the content of crocin-1 in gardenia yellow reached 29.6%, while geniposide in another fraction reached 83.4%. An extract of crocins was obtained from gardenia yellow in a second-stage separation using RP medium-pressure LC, with its color value to be 756 and the content of crocin-1 reaching 60.8%. The separation process was highly efficient, low cost, and compact, which may be informative for purifications of other natural products from complex plant extracts. PMID:24259446

  11. Gravity-Driven Thin Film Flow of an Ellis Fluid

    PubMed Central

    Kheyfets, Vitaly O.

    2014-01-01

    The thin film lubrication approximation has been studied extensively for moving contact lines of Newtonian fluids. However, many industrial and biological applications of the thin film equation involve shear-thinning fluids, which often also exhibit a Newtonian plateau at low shear. This study presents new numerical simulations of the three-dimensional (i.e. two-dimensional spreading), constant-volume, gravity-driven, free surface flow of an Ellis fluid. The numerical solution was validated with a new similarity solution, compared to previous experiments, and then used in a parametric study. The parametric study centered around rheological data for an example biological application of thin film flow: topical drug delivery of anti-HIV microbicide formulations, e.g. hydroxyethylcellulose (HEC) polymer solutions. The parametric study evaluated how spreading length and front velocity saturation depend on Ellis parameters. A lower concentration polymer solution with smaller zero shear viscosity (η0), τ1/2, and λ values spread further. However, when comparing any two fluids with any possible combinations of Ellis parameters, the impact of changing one parameter on spreading length depends on the direction and magnitude of changes in the other two parameters. In addition, the isolated effect of the shear-thinning parameter, λ, on the front velocity saturation depended on τ1/2. This study highlighted the relative effects of the individual Ellis parameters, and showed that the shear rates in this flow were in both the shear-thinning and plateau regions of rheological behavior, emphasizing the importance of characterizing the full range of shear-rates in rheological measurements. The validated numerical model and parametric study provides a useful tool for future steps to optimize flow of a fluid with rheological behavior well-described by the Ellis constitutive model, in a range of industrial and biological applications. PMID:25309029

  12. Gravity-Driven Thin Film Flow of an Ellis Fluid.

    PubMed

    Kheyfets, Vitaly O; Kieweg, Sarah L

    2013-12-01

    The thin film lubrication approximation has been studied extensively for moving contact lines of Newtonian fluids. However, many industrial and biological applications of the thin film equation involve shear-thinning fluids, which often also exhibit a Newtonian plateau at low shear. This study presents new numerical simulations of the three-dimensional (i.e. two-dimensional spreading), constant-volume, gravity-driven, free surface flow of an Ellis fluid. The numerical solution was validated with a new similarity solution, compared to previous experiments, and then used in a parametric study. The parametric study centered around rheological data for an example biological application of thin film flow: topical drug delivery of anti-HIV microbicide formulations, e.g. hydroxyethylcellulose (HEC) polymer solutions. The parametric study evaluated how spreading length and front velocity saturation depend on Ellis parameters. A lower concentration polymer solution with smaller zero shear viscosity (η 0), τ 1/2, and λ values spread further. However, when comparing any two fluids with any possible combinations of Ellis parameters, the impact of changing one parameter on spreading length depends on the direction and magnitude of changes in the other two parameters. In addition, the isolated effect of the shear-thinning parameter, λ, on the front velocity saturation depended on τ 1/2. This study highlighted the relative effects of the individual Ellis parameters, and showed that the shear rates in this flow were in both the shear-thinning and plateau regions of rheological behavior, emphasizing the importance of characterizing the full range of shear-rates in rheological measurements. The validated numerical model and parametric study provides a useful tool for future steps to optimize flow of a fluid with rheological behavior well-described by the Ellis constitutive model, in a range of industrial and biological applications. PMID:25309029

  13. Metabolic profile of Fructus Gardeniae in human plasma and urine using ultra high-performance liquid chromatography coupled with high-resolution LTQ-orbitrap mass spectrometry.

    PubMed

    Wang, Gao-Wa; Bao, Burenbatu; Han, Zhi-Qiang; Han, Qing-Yu; Yang, Xiu-Lan

    2016-10-01

    1. In China, Fructus Gardeniae was used as a traditional Chinese medicine (TCM) with a wide array of biological activities. The bioactive components identified in Fructus Gardeniae mainly included iridoids, flavonids, pigments, and so on. Among them, iridoids were regarded as important compounds in Fructus Gardeniae. Though analyses of the constituents in biological samples after oral administration of Fructus Gardeniae effective fraction or its active compounds have been reported, few efforts have been made to investigate the metabolic profile of Fructus Gardeniae in humans. In this study, the constituents and metabolites of Fructus Gardeniae in human blood and urine after oral administration of Fructus Gardeniae were investigated using ultra high-performance liquid chromatography (UHPLC) coupled with high-resolution LTQ-Orbitrap mass spectrometery. 2. Totally, 14 constituents (two parent compounds and 12 metabolites) of Fructus Gardeniae were identified in human plasma and urine either by comparing the retention time and mass spectrometry data with that of reference compounds or by the accurate high-resolution MS/MS data of the chemicals. The compounds identified were mainly iridoid glycosides such as geniposide and the derivatives of genipin-O-glucuronide. Among them, 11 metabolites were detected in human plasma and urine while the other three metabolites including geniposidic acid (M1), demethylation derivative of genipin-O-glucuronide (M2), and dehydration product of mono-hydroxylated genipin-O-glucuronide (M9) were only discovered in human urine. Further, the possible metabolic pathways of Fructus Gardeniae in vivo were proposed and the peak area-time curve of the most abundant metabolite genipin-O-glucuronide (M13) in human plasma after oral administration of Fructus Gardeniae was depicted. The results suggested that a metabolic difference existed between rats and humans. 3. The results obtained in the present research would provide basic information to

  14. Evolving science in adolescence: comment on Ellis et al. (2012).

    PubMed

    Dodge, Kenneth A; Albert, Dustin

    2012-05-01

    Ellis et al. (2012) bring an evolutionary perspective to bear on adolescent risky behavioral development, clinical practice, and public policy. The authors offer important insights that (a) some risky behaviors may be adaptive for the individual and the species by being hard-wired due to fitness benefits and (b) interventions might be more successful if they move with, rather than against, the natural tendencies of an adolescent. Ellis and colleagues criticize the field of developmental psychopathology, but we see the 2 fields as complementary. Their position would be enhanced by integrating it with contemporary perspectives on dynamic cascades through which normative behavior turns into genuinely maladaptive outcomes, dual processes in adolescent neural development, and adolescent decision making. Finally, they rightly note that innovation is needed in interventions and policies toward adolescent problem behavior.

  15. Gravitational Microlensing by Ellis Wormhole: Second Order Effects

    NASA Astrophysics Data System (ADS)

    Lukmanova, Regina; Kulbakova, Aliya; Izmailov, Ramil; Potapov, Alexander A.

    2016-07-01

    Gravitational lensing is the effect of light bending in a gravitational field. It can be used as a possible observational method to detect or exclude the existence of wormholes. In this work, we extend the work by Abe on gravitational microlensing by Ellis wormhole by including the second order deflection term. Using the lens equation and definition of Einstein radius, we find the angular locations of the physical image inside and outside Einstein ring. The work contains a comparative analysis of light curves between the Schwarzschild black hole and the Ellis wormhole that can be used to distinguish such objects though such distinctions are too minute to be observable even in the near future. We also tabulate the optical depth and event rate for lensing by bulge and Large Magellanic Cloud (LMC) stars.

  16. Cytotoxic, antitopoisomerase IIα, and anti-HIV-1 activities of triterpenoids isolated from leaves and twigs of Gardenia carinata.

    PubMed

    Kongkum, Naowarat; Tuchinda, Patoomratana; Pohmakotr, Manat; Reutrakul, Vichai; Piyachaturawat, Pawinee; Jariyawat, Surawat; Suksen, Kanoknetr; Akkarawongsapat, Radeekorn; Kasisit, Jitra; Napaswad, Chanita

    2013-04-26

    Eight new cycloartane triterpenoids (1-8), named carinatins A-H, and the known compounds secaubryolide (9) and dikamaliartane D (10) were isolated from the leaves and twigs of Gardenia carinata. Their structures were determined on the basis of spectroscopic methods. Cytotoxic, antitopoisomerase IIα, and anti-HIV-1 activities of compounds 1-7, 9, and 10 were investigated.

  17. 75 FR 38768 - Rehabilitation of Floodwater Retarding Structure No. 10 of the Mountain Creek Watershed, Ellis...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ... Mountain Creek Watershed, Ellis County, TX AGENCY: Natural Resources Conservation Service. ACTION: Notice... prepared for the rehabilitation of Floodwater Retarding Structure No. 10 of the Mountain Creek...

  18. Rational Emotive Behavioural Therapy: The Evolution of a Revolution: Interview With Dr. Debbie Joffe Ellis, Work Partner and Wife of Dr. Albert Ellis, the Creator of REBT.

    PubMed

    Ellis, Debbie Joffe; Rovira, Montse

    2015-02-01

    Recognized as one of the most influential thinkers and psychologists, Albert Ellis PhD (1913-2007) revolutionized Psychology when he created the first cognitive psychotherapy, Rational Emotive Behavioral Therapy. After he passed away, Dr. Debbie Joffe Ellis continues spreading his legacy around the world. Psychologist, lecturer, writer, trainer, she dedicates her life to disseminate REBT and extend it through different statements, from the social to the educational, from the academic to the clinical. In this interview, she goes through her own history and her husband's one, bringing us closer to understanding Albert Ellis as the leading figure in his field, and the oneness they experienced through their professional and personal relationship.

  19. 78 FR 74009 - Safety Zone; Nike Fireworks, Upper New York Bay, Ellis Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ..., 2011 (76 FR 69614). ] Table 1 1. Nike Fireworks Ellis Launch site: A barge located Island Safety Zone... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 Safety Zone; Nike Fireworks, Upper New York Bay, Ellis Island,...

  20. Dandy-Walker malformation in Ellis-van Creveld syndrome.

    PubMed

    Zangwill, K M; Boal, D K; Ladda, R L

    1988-09-01

    We report on 2 Old Order Amish patients with Ellis-van Creveld (EvC) syndrome and the Dandy-Walker malformation; a similar case is noted in the literature. Pedigree analysis of our patients documents extensive inbreeding in successive generations. Considering the rarity of EvC syndrome and Dandy-Walker malformation as isolated malformations, the appearance of both in our 2 patients plus the patient in the literature suggests that Dandy-Walker malformation may be a manifestation in the EvC syndrome. However, in this isolate the coincidental occurrence of 2 rare recessive traits cannot be excluded. PMID:3223493

  1. Ellis-van Creveld syndrome: A rare clinical entity.

    PubMed

    Kamal, Reet; Dahiya, Parveen; Kaur, Simerpreet; Bhardwaj, Rohit; Chaudhary, Karun

    2013-01-01

    Ellis-van Creveld (EVC) syndrome is a genetic disorder with autosomal recessive transmission, which may clinically present as small stature, short limbs, fine sparse hair, hypoplastic fingernails, multiple musculofibrous frenula, conical teeth, hypoplasia of the enamel, hypodontia, and malocclusion. Heart defects, especially abnormalities of atrial septation, have been found in about 60% of cases. The mutation in EVC and EVC2 gene is responsible for this syndrome. The presence of multiple orodental findings makes this syndrome important for dentists. The aim of this article is to present a rare case of EVC syndrome in a 10-year-old girl along with the review of literature.

  2. Ellis-van Creveld Syndrome with Sagittal Craniosynostosis.

    PubMed

    Fischer, Andrew S; Weathers, William M; Wolfswinkel, Erik M; Bollo, Robert J; Hollier, Larry H; Buchanan, Edward P

    2015-06-01

    Ellis-van Creveld syndrome (EVC) is a rare disorder (the incidence is estimated at around 7/1,000,000) characterized by the clinical tetrad of chondrodystrophy, polydactyly, ectodermal dysplasia, and cardiac anomalies. Sagittal synostosis is characterized by a dolichocephalic head shape resulting from premature fusion of the sagittal suture. Both are rare disorders, which have never been reported together. We present a case of EVC and sagittal synostosis. We report the clinical features of a Hispanic boy with EVC and sagittal craniosynostosis who underwent cranial vault remodeling. The presentation of this patient is gone over in detail. A never before reported case of EVC and sagittal synostosis is presented in detail.

  3. Evaluation of gardenia yellow using crocetin from alkaline hydrolysis based on ultra high performance liquid chromatography and high-speed countercurrent chromatography.

    PubMed

    Inoue, Koichi; Tanada, Chihiro; Nishikawa, Hiroaki; Matsuda, Satoru; Tada, Atsuko; Ito, Yusai; Min, Jun Zhe; Todoroki, Kenichiro; Sugimoto, Naoki; Toyo'oka, Toshimasa; Akiyama, Hiroshi

    2014-12-01

    Gardenia yellow is globally the most valuable spice and food color. It is generally a mixture of water-soluble carotenoid glycosyl esters which consist of crocetin bis(gentiobiosyl) ester as the main component. Crocetin is a natural carotenoid dicarboxylic acid that may be a candidate drug for pharmaceutical development, however, it is either present in trace amounts or is absent in natural gardenia yellow products. We here propose that crocetin produced by alkaline hydrolysis can be used to qualitatively evaluate gardenia yellow products using an ultra high performance liquid chromatographic assay. A useful and efficient isolation technique for isolating high-purity crocetin from gardenia yellow using high-speed countercurrent chromatography is described. High-speed countercurrent chromatographic fractionation followed by an ultra high performance liquid chromatographic assay showed that trans-crocetin is easily converted to about 15% cis-crocetin (85% trans-crocetin). Crocetin in gardenia yellow was quantitatively evaluated. Our approach is based on the hydrolysis process for converting crocetin glycosyl esters to crocetin before evaluation and isolation using the ultra high performance liquid chromatographic and high-speed countercurrent chromatographic methods. The combination of hydrolysis and chromatographic methods allows evaluation of the purity and quantity of crocetin in gardenia yellow.

  4. Astrometric Image Centroid Displacements due to Gravitational Microlensing by the Ellis Wormhole

    NASA Astrophysics Data System (ADS)

    Toki, Yukiharu; Kitamura, Takao; Asada, Hideki; Abe, Fumio

    2011-10-01

    Continuing work initiated in an earlier publication, we study the gravitational microlensing effects of the Ellis wormhole in the weak-field limit. First, we find a suitable coordinate transformation, such that the lens equation and analytic expressions of the lensed image positions can become much simpler. Second, we prove that two images always appear for the weak-field lens by the Ellis wormhole. By using these analytic results, we discuss astrometric image centroid displacements due to gravitational microlensing by the Ellis wormhole. The astrometric image centroid trajectory by the Ellis wormhole is different from the standard one by a spherical lensing object that is expressed by the Schwarzschild metric. The anomalous shift of the image centroid by the Ellis wormhole lens is smaller than that by the Schwarzschild lens, provided that the impact parameter and the Einstein ring radius are the same. Therefore, the lensed image centroid by the Ellis wormhole moves slower. Such a difference, although it is very small, will be, in principle, applicable for detecting or constraining the Ellis wormhole by using future high-precision astrometry observations. In particular, the image centroid position gives us additional information, so that the parameter degeneracy existing in photometric microlensing can be partially broken. The anomalous shift reaches the order of a few micro arcseconds, if our galaxy hosts a wormhole with throat radius larger than 105 km. When the source moves tangentially to the Einstein ring, for instance, the maximum position shift of the image centroid by the Ellis wormhole is 0.18 normalized by the Einstein ring radius. For the same source trajectory, the maximum difference between the centroid displacement by the Ellis wormhole lens and that by the Schwarzschild one with the same Einstein ring radius is -0.16 in the units of the Einstein radius, where the negative means that the astrometric displacement by the Ellis wormhole lens is smaller

  5. ASTROMETRIC IMAGE CENTROID DISPLACEMENTS DUE TO GRAVITATIONAL MICROLENSING BY THE ELLIS WORMHOLE

    SciTech Connect

    Toki, Yukiharu; Kitamura, Takao; Asada, Hideki; Abe, Fumio

    2011-10-20

    Continuing work initiated in an earlier publication, we study the gravitational microlensing effects of the Ellis wormhole in the weak-field limit. First, we find a suitable coordinate transformation, such that the lens equation and analytic expressions of the lensed image positions can become much simpler. Second, we prove that two images always appear for the weak-field lens by the Ellis wormhole. By using these analytic results, we discuss astrometric image centroid displacements due to gravitational microlensing by the Ellis wormhole. The astrometric image centroid trajectory by the Ellis wormhole is different from the standard one by a spherical lensing object that is expressed by the Schwarzschild metric. The anomalous shift of the image centroid by the Ellis wormhole lens is smaller than that by the Schwarzschild lens, provided that the impact parameter and the Einstein ring radius are the same. Therefore, the lensed image centroid by the Ellis wormhole moves slower. Such a difference, although it is very small, will be, in principle, applicable for detecting or constraining the Ellis wormhole by using future high-precision astrometry observations. In particular, the image centroid position gives us additional information, so that the parameter degeneracy existing in photometric microlensing can be partially broken. The anomalous shift reaches the order of a few micro arcseconds, if our galaxy hosts a wormhole with throat radius larger than 10{sup 5} km. When the source moves tangentially to the Einstein ring, for instance, the maximum position shift of the image centroid by the Ellis wormhole is 0.18 normalized by the Einstein ring radius. For the same source trajectory, the maximum difference between the centroid displacement by the Ellis wormhole lens and that by the Schwarzschild one with the same Einstein ring radius is -0.16 in the units of the Einstein radius, where the negative means that the astrometric displacement by the Ellis wormhole lens is

  6. Rational emotive therapy-a study of initial therapy sessions of Albert Ellis.

    PubMed

    Becker, I M; Rosenfeld, J G

    1976-10-01

    Because psychotherapy is what a therapist does, and not necessarily what he says he does, it is important to observe the activity of leaders in the field during their sessions. Twenty taped initial psychotherapy sessions by Albert Ellis were selected randomly from 70 recently recorded ones. Typescripts of each session were made, and two raters naive to the purposes were trained to place each of Ellis' statements into 1 of 17 categories. Each category consisted of a therapeutic technique. Some of these were ones that Ellis did during the 20 sessions examined was related very closely to what he has claimed to do, but that he did vary considerably from client to client.

  7. Ellis-van Creveld syndrome: a case report.

    PubMed

    Shah, B; Ashok, L; Sujatha, G P

    2008-01-01

    Ellis-van Creveld syndrome (EvC) is a disease complex, where all the three embryonic layers appear to be involved. This disorder is also called as Chondroectodermal dysplasia. EvC is an autosomal recessive disorder resulting from mutations in these patients. Mutations in the two genes EVC and EVC2, have been identified to cause the condition. It has been considered as a skeletal dysplasia with an incidence of approximately 1 out of 1,50,000 live births. A high prevalence has been reported among certain populations like Amish and Arabs of Gaza strip. There are more than 300 cases of EvC reported into the literature. About 50-60% of cases have been reported with congenital cardiac malformations. PMID:18974540

  8. Ellis-van Creveld Syndrome: Mutations Uncovered in Lebanese Families.

    PubMed

    Valencia, Maria; Tabet, Lara; Yazbeck, Nadine; Araj, Alia; Ruiz-Perez, Victor L; Charaffedine, Khalil; Fares, Farah; Badra, Rebecca; Farra, Chantal

    2015-01-01

    Background. Ellis-van Creveld (EvC) syndrome is a rare, autosomal recessive disorder characterized by short stature, short limbs, growth retardation, polydactyly, and ectodermal defects with cardiac anomalies occurring in around 60% of cases. EVC syndrome has been linked to mutations in EVC and EVC2 genes. Case Presentation. We report EvC syndrome in two unrelated Lebanese families both having homozygous mutations in the EVC2 gene, c.2653C>T (p.(Arg885(*))) and c.2012_2015del (p.(Leu671(*))) in exons 15 and 13, respectively, with the latter being reported for the first time. Conclusion. Although EvC has been largely described in the medical literature, clinical features of this syndrome vary. While more research is required to explore other genes involved in EvC, early diagnosis and therapeutic care are important to achieve a better quality of life.

  9. Ellis-van Creveld syndrome: a case report.

    PubMed

    Shah, B; Ashok, L; Sujatha, G P

    2008-01-01

    Ellis-van Creveld syndrome (EvC) is a disease complex, where all the three embryonic layers appear to be involved. This disorder is also called as Chondroectodermal dysplasia. EvC is an autosomal recessive disorder resulting from mutations in these patients. Mutations in the two genes EVC and EVC2, have been identified to cause the condition. It has been considered as a skeletal dysplasia with an incidence of approximately 1 out of 1,50,000 live births. A high prevalence has been reported among certain populations like Amish and Arabs of Gaza strip. There are more than 300 cases of EvC reported into the literature. About 50-60% of cases have been reported with congenital cardiac malformations.

  10. Synthesis of porous starch xerogels modified with mercaptosuccinic acid to remove hazardous gardenia yellow.

    PubMed

    Bao, Liping; Zhu, Xinyi; Dai, Hongxia; Tao, Yongxin; Zhou, Xiaoying; Liu, Wenjie; Kong, Yong

    2016-08-01

    Mercaptosuccinic acid (MSA) molecules were inserted into potato starch, leading to the breaking of intrinsic H-bonds within macromolecular chains of starch and the formation of intermolecular H-bonds between MSA and starch, which could be verified by Fourier transform infrared spectroscopy (FT-TR). MSA modified porous starch xerogels (PSX/MSA) were obtained after freeze-drying the MSA modified starch, and they were characterized by field emission scanning electron microscopy (FESEM), exhibiting the intriguing porous structure due to the separation of starch chains by MSA molecules. The PSX/MSA were then used as the adsorbents to remove gardenia yellow (GY), a natural colorant with genotoxicity. Due to the porous structure of PSX and the introduced carboxyl groups from MSA, the adsorption capacity of the PSX/MSA was much higher than that of the starch xerogels alone (SX). The adsorption behaviors of GY by the PSX/MSA fitted both the Freundlich isotherm model and the pseudo-second-order kinetic model, and the efficient adsorption of GY suggested that the PSX/MSA might be potential adsorbents for the removal of dyes from contaminated aquatic systems. PMID:27151673

  11. An empirical investigation of Albert Ellis's binary model of distress.

    PubMed

    David, Daniel; Montgomery, Guy H; Macavei, Bianca; Bovbjerg, Dana H

    2005-04-01

    In the current literature, distress is typically described according to a unitary model: High levels of distress are conceptualized as a high level of negative affect while low levels of distress are typically conceptualized as a low level of negative affect. On the other hand, Albert Ellis (1994) and some of his rational-emotive and cognitive-behavioral professional colleagues have more recently described distress as a binary construct composed of two different components: functional negative feelings (e.g., sad) and dysfunctional negative feelings (e.g., worthless). In two studies using 55 U.S. breast-cancer patients and 45 Romanian breast-cancer patients, respectively, we compared hypotheses derived from unitary and binary models of distress. The results revealed that in a stressful situation (i.e., upcoming breast surgery) high levels of irrational beliefs were associated with a high level of both functional and dysfunctional negative feelings while low levels of irrational beliefs were associated with a low level of dysfunctional negative feelings and a high level of functional negative feelings. Thus, that support for the binary model of distress found in both U.S. and Romanian samples suggests both the robustness and the generalizability of the results. Theoretical and practical implications are discussed.

  12. Growth charts for children with Ellis-van Creveld syndrome.

    PubMed

    Verbeek, Sabine; Eilers, Paul H C; Lawrence, Kate; Hennekam, Raoul C M; Versteegh, Florens G A

    2011-02-01

    Ellis-van Creveld (EvC) syndrome is a congenital malformation syndrome with marked growth retardation. In this study, specific growth charts for EvC patients were derived to allow better follow-up of growth and earlier detection of growth patterns unusual for EvC. With the use of 235 observations of 101 EvC patients (49 males, 52 females), growth charts for males and females from 0 to 20 years of age were derived. Longitudinal and cross-sectional data were collected from an earlier review of growth data in EvC, a database of EvC patients, and from recent literature. To model the growth charts, the GAMLSS package for the R statistical program was used. Height of EvC patients was compared to healthy children using Dutch growth charts. Data are presented both on a scale for age and on a scale for the square root of age. Compared to healthy Dutch children, mean height standard deviation score values for male and female EvC patients were -3.1 and -3.0, respectively. The present growth charts should be useful in the follow-up of EvC patients. Most importantly, early detection of growth hormone deficiency, known to occur in EvC, will be facilitated.

  13. [Optimization of vacuum belt drying process of Gardeniae Fructus in Reduning injection by Box-Behnken design-response surface methodology].

    PubMed

    Huang, Dao-sheng; Shi, Wei; Han, Lei; Sun, Ke; Chen, Guang-bo; Wu Jian-xiong; Xu, Gui-hong; Bi, Yu-an; Wang, Zhen-zhong; Xiao, Wei

    2015-06-01

    To optimize the belt drying process conditions optimization of Gardeniae Fructus extract from Reduning injection by Box-Behnken design-response surface methodology, on the basis of single factor experiment, a three-factor and three-level Box-Behnken experimental design was employed to optimize the drying technology of Gardeniae Fructus extract from Reduning injection. With drying temperature, drying time, feeding speed as independent variables and the content of geniposide as dependent variable, the experimental data were fitted to a second order polynomial equation, establishing the mathematical relationship between the content of geniposide and respective variables. With the experimental data analyzed by Design-Expert 8. 0. 6, the optimal drying parameter was as follows: the drying temperature was 98.5 degrees C , the drying time was 89 min, the feeding speed was 99.8 r x min(-1). Three verification experiments were taked under this technology and the measured average content of geniposide was 564. 108 mg x g(-1), which was close to the model prediction: 563. 307 mg x g(-1). According to the verification test, the Gardeniae Fructus belt drying process is steady and feasible. So single factor experiments combined with response surface method (RSM) could be used to optimize the drying technology of Reduning injection Gardenia extract.

  14. Oral manifestations in Ellis-van Creveld syndrome: report of five cases.

    PubMed

    Cahuana, Abel; Palma, Camila; Gonzáles, Wilber; Geán, Esther

    2004-01-01

    Ellis-van Creveld syndrome, or chondroectodermal dysplasia, is an autosomal recessive disorder with characteristic clinical manifestations. Its incidence in the general population is low. The oral manifestations of Ellis-van Creveld are found in soft tissues and teeth, but the dental literature on the subject is scarce. In the last 20 years, 5 cases of Ellis-van Creveld syndrome have been followed at the Pediatric Dentistry Service of the Hospital Sant Joan de Déu, Barcelona. The present study describes the constant and variable oral findings in these patients, which play an important role in the diagnosis criteria for the syndrome. The presence of a great variety of oral manifestations such as fusion of the upper lip to the gingival margin, presence of multiple frenula, abnormally shaped and microdontic teeth, and congenitally missing teeth requires multidisciplinary dental treatment, with consideration for the high incidence of cardiac defects in these patients. PMID:15185812

  15. Optimization of ultrasound-assisted extraction of gardenia fruit oil with bioactive components and their identification and quantification by HPLC-DAD/ESI-MS(2).

    PubMed

    Cai, Xiaoshuang; Zhang, Rui; Guo, Ying; He, Jingren; Li, Shuyi; Zhu, Zhenzhou; Liu, Gang; Liu, Zhiwei; Yang, Jun

    2015-07-01

    Compounds in Fructus Gardeniae have been shown to possess a wide array of biological activities. However, Gardenia oil extracted from its fruit is less reported and its composition remains uncertain. To completely characterize lipophilic compounds in Gardenia oil, three conventional extraction (CE) and ultrasound-assisted extraction (UAE) methods were investigated. The oil extraction yield obtained by UAE was 51.8% higher than that acquired by cold-pressed extraction (CPE). The fatty acid profile in UAE oil with different solvents was characterized by GC-MS. Petroleum ether was observed to be an ideal solvent with 8.59% extraction yield and 78.88% recovery rate and with a ratio of 3.11 of unsaturated fatty acids to saturated fatty acids. Response surface methodology (RSM) with Box-Behnken Design (BBD) was applied to optimize conditions in UEA of oil to maximize extraction yield. Furthermore, the bioactive components in oil extracted by UAE were qualitatively identified and quantified by HPLC-DAD/ESI-MS(2) and HPLC-DAD analysis. The eight compounds in Gardenia oil, including geniposide, trans/cis-crocin-1, crocin-2, crocin-3, crocin-4, and trans/cis-crocetin, were structurally revealed. The corresponding transfer rates of the bioactive components showed that the lipophilic trans/cis-crocetin could be completely transferred from fruit to oil, with the highest concentration of 11.38 μg g(-1) oil among all compounds quantified. These findings could deliver potential application to a large-scale production of functional Gardenia oil whose bioactive components possess health benefits. PMID:26040554

  16. Research on the change of chemical composition in productive process of Re Du Ning injection by HPLC/Q-TOF MS.

    PubMed

    Zhang, Shan; Li, Yan-Jing; Zhang, Chun-Xiao; Huang, Wen-Zhe; Ding, Gang; Wang, Zhen-Zhong; Bi, Yu-An; Xiao, Wei

    2016-02-01

    A high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) was developed for the analysis of chemical composition change in the production process of Re Du Ning injection, a Chinese medicine preparation with a combination of Lonicera japonica Thunb., Gardenia jasminoides Ellis and Artemisia annua L. A total of 90 compounds from raw materials-intermediates-Re Du Ning injection were detected; among them, 55 compounds were identified or tentatively characterized, and the characteristic ions of different types of compounds were described. Based on these studies, the different types of compounds in the various process routes were analyzed. A total of 28 compounds, including seven iridoid glycosides and six monoterpenes from G. jasminoides Ellis, five iridoid glycosides, nine phenolic acids and one unknown compound from L. japonica Thunb., were transferred to Re Du Ning injection, and two unknown compounds were generated in the production process of Re Du Ning injection. The results indicated that the Chinese Medicine Pharmaceutical process control is very important. This method could provide some reference for other Chinese medicine preparations.

  17. Research on the change of chemical composition in productive process of Re Du Ning injection by HPLC/Q-TOF MS.

    PubMed

    Zhang, Shan; Li, Yan-Jing; Zhang, Chun-Xiao; Huang, Wen-Zhe; Ding, Gang; Wang, Zhen-Zhong; Bi, Yu-An; Xiao, Wei

    2016-02-01

    A high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) was developed for the analysis of chemical composition change in the production process of Re Du Ning injection, a Chinese medicine preparation with a combination of Lonicera japonica Thunb., Gardenia jasminoides Ellis and Artemisia annua L. A total of 90 compounds from raw materials-intermediates-Re Du Ning injection were detected; among them, 55 compounds were identified or tentatively characterized, and the characteristic ions of different types of compounds were described. Based on these studies, the different types of compounds in the various process routes were analyzed. A total of 28 compounds, including seven iridoid glycosides and six monoterpenes from G. jasminoides Ellis, five iridoid glycosides, nine phenolic acids and one unknown compound from L. japonica Thunb., were transferred to Re Du Ning injection, and two unknown compounds were generated in the production process of Re Du Ning injection. The results indicated that the Chinese Medicine Pharmaceutical process control is very important. This method could provide some reference for other Chinese medicine preparations. PMID:26058547

  18. Welcome Home Annie: Rethinking Ellis Island and Annie Moore in the Classroom

    ERIC Educational Resources Information Center

    Mercurio, Mia

    2008-01-01

    The story of the United States and the people who have made it their home would not be complete without considering the experience of Irish immigrants--particularly the experience of Annie Moore, the first immigrant to be processed on Ellis Island. However, the story of Annie Moore, and how it has been recounted and taught to date, is inaccurate.…

  19. Ellis-van Creveld syndrome: report of 15 cases in an inbred kindred.

    PubMed Central

    da Silva, E O; Janovitz, D; de Albuquerque, S C

    1980-01-01

    An inbred kindred with 15 cases of the autosomal recessive Ellis-van Creveld syndrome is reported. The ages of the 12 living affected varied between 3 and 82 years. The main characteristics include polydactyly of the hands and feet and several other skeletal anomalies, oral manifestations, and malformations of the heart in 50% of the living affected. Images PMID:7218275

  20. Productive and Reproductive Work on the Family Farm: Changes Among Ethnic Groups in Ellis County, Kansas.

    ERIC Educational Resources Information Center

    Flora, Cornelia Butler; Stitz, John

    This report is based on data obtained from historical documents, quantitative analysis of state agricultural censuses for 1885, 1895, and 1905, and interviews with farm women of Volga and German heritages, aged 14 to 87. The participation of women in wheat-based farming systems in Ellis County, Kansas, is examined as related to the ethnic…

  1. Rational Emotive Therapy--A Study of Initial Therapy Sessions of Albert Ellis

    ERIC Educational Resources Information Center

    Becker, Irving M.; Rosenfeld, Joseph G.

    1976-01-01

    The purpose of this research was to see which specific techniques Albert Ellis, the founder of the school of therapy known as Rational Emotive Therapy, uses during an initial therapy session and also to see what percentage of time each technique was utilized. (Author)

  2. The Importance of Voice in Supervision: A Response to Ellis and Robbins (1993) and Bernstein (1993).

    ERIC Educational Resources Information Center

    Twohey, Denise

    1993-01-01

    Comments two responses to author's article "Listening for the Voices of Care and Justice in Counselor Supervision" (Twohey and Volker, 1993). Responds to Ellis and Robbins (1993) by clarifying perspective on relationship between moral decision making and supervision. Takes issue with Bernstein's (1993) comments about superiority of instrumental…

  3. Overgeneralization from a Narrow Focus: A Response to Ellis et al. (2008) and Bitchener (2008)

    ERIC Educational Resources Information Center

    Xu, Cuiqin

    2009-01-01

    This paper presents a critical analysis of two recent studies on error correction, Ellis, Sheen, Murakami, and Takashima (2008) and Bitchener (2008) and argues that neither study constitutes evidence for error correction based on discussion of the comparability of participants, design issues, and analysis flaws in both studies. The paper further…

  4. 77 FR 66979 - Ellis Road/American Electric Corporation, Jacksonville, Duval County, FL, Notice of Settlement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-08

    ... Ms. Paula V. Painter. Submit your comments by Site name Ellis Road/American Electric Corporation Site... . Email. Painter.Paula@epa.gov . U.S. Environmental Protection Agency, 61 Forsyth Street SW., Atlanta, Georgia 30303. FOR FURTHER INFORMATION CONTACT: Paula V. Painter at 404/562-8887. Dated: October 18,...

  5. Endorsements of Ellis' Irrational Beliefs as a Function of DSM-III Psychotic Diagnosis.

    ERIC Educational Resources Information Center

    Newark, Charles S.; Whitt, J. Kenneth

    1983-01-01

    Examined opinions of normal subjects (N=120) and patients diagnosed as manic and schizophrenic (N=150) regarding Ellis's 11 irrational beliefs. Results showed that the schizophrenia and major depressive groups endorsed three and four irrational beliefs respectively with no endorsement from the normal and manic groups. (LLL)

  6. An Interview with...Ed Ellis: Working To Improve Education for Adolescents with Learning Disabilities.

    ERIC Educational Resources Information Center

    Casareno, Alexander

    2002-01-01

    An interview with Edwin S. Ellis, an expert on cognitive-based interventions and curriculum for students with learning disabilities (LD) focuses on the roots of his interest in strategic learning, the teacher's role in effective schooling, the need to go beyond labels, knowledge elaboration in learning, effective learning strategies for adolescent…

  7. Supervisory Theory into Practice: Differential Perception of Supervision by Ekstein, Ellis, Polster, and Rogers.

    ERIC Educational Resources Information Center

    Goodyear, Rodney K.; And Others

    1984-01-01

    Compared the supervision that four psychologists (Ekstein, Ellis, Polster, and Rogers) each provided to the same supervisee. Subjects were 58 counseling supervisors who evaluated videotaped supervision sessions. Results showed the supervisors were perceived as differing in attractiveness, role, and supervisory methods, generally consistent with…

  8. Bioavailability and Brain-Targeting of Geniposide in Gardenia-Borneol Co-Compound by Different Administration Routes in Mice

    PubMed Central

    Lu, Yang; Du, Shouying; Bai, Jie; Li, Pengyue; Wen, Ran; Zhao, Xuejiao

    2012-01-01

    Both geniposide (Ge) and borneol (Bo) are bioactive substances derived from traditional Chinese medicine. Injections containing co-compound of Gardenia-Borneol are widely used for stroke treatment in China, such as “Xingnaojing” multi-component injection. As more and more adverse reactions (especially drug allergy) were reported, it is urgent to find more effective and safer routes of administration for such kinds of medicines. In this paper, bioavailabilities and brain-target effects of geniposide in Gardenia-Borneol co-compound through different administration routes in mice were investigated. Geniposide concentrations in plasma and in brain of mice were determined by reversed-phase high-performance liquid chromatography. The pharmacokinetics parameters of intranasal (i.n.) and intragastric (i.g.) administration were compared with intravenous (i.v.) administration. The bioavailabilities of Ge were 85.38% and 28.76% for i.n. and i.g. while Tmax were 1 min and 30 min. Cmax were 21.881 ± 5.398, 1.914 ± 0.327 and 42.410 ± 6.268 μg/mL for i.n., i.g. and i.v., respectively. The AUC of Ge in brain were 32413.6 ± 4573.9, 6440.1 ± 863.7 and 37270.5 ± 4160.6 ng/g·min for i.n., i.g. and i.v., respectively. The drug target indexes (DTI) were 1.02 and 0.60 for i.n. and i.g. The results demonstrated that geniposide could be absorbed promptly and thoroughly by i.n. administration in mice and basically transported into the brain though blood vessel passways. PMID:23203054

  9. Stability and instability of Ellis and phantom wormholes: Are there ghosts?

    NASA Astrophysics Data System (ADS)

    Nandi, K. K.; Potapov, A. A.; Izmailov, R. N.; Tamang, A.; Evans, J. C.

    2016-05-01

    It is concluded in the literature that the Ellis wormhole is unstable under small perturbations and would either decay to the Schwarzschild black hole or expand away to infinity. While this deterministic conclusion of instability is correct, we show that the Ellis wormhole reduces to the Schwarzschild black hole only when the Ellis solution parameter γ assumes a complex value -i . We shall then reexamine the stability of Ellis and phantom wormholes from the viewpoint of local and asymptotic observers by using a completely different approach, viz., we adapt Tangherlini's nondeterministic, prequantal statistical simulation about photon motion in the real optical medium to an effective medium reformulation of motions obtained via Hamilton's optical-mechanical analogy in a gravity field. A crucial component of Tangherlini's idea is the observed increase of momentum of the photons entering a real medium. We show that this fact has a heuristic parallel in the effective medium version of the Pound-Rebka experiment in gravity. Our conclusion is that there is a nonzero probability that Ellis and phantom wormholes could appear stable or unstable depending on the location of observers and on the values of γ , leading to the possibility of ghost wormholes (like ghost stars). The Schwarzschild horizon, however, would always certainly appear to be stable (R =1 , T =0 ) to observers regardless of their location. Phantom wormholes of bounded mass in the extreme limit a →-1 are also shown to be stable just as the Schwarzschild black hole is. We shall propose a thought experiment showing that our nondeterministic results could be numerically translated into observable deterministic signatures of ghost wormholes.

  10. Screening and identification of multi-components in Re Du Ning injections using LC/TOF-MS coupled with UV-irradiation.

    PubMed

    Li, Lingzhi; Wang, Zhenzhong; Peng, Ying; Fu, Xiaohuan; Wang, Yongxiang; Xiao, Wei; Song, Shaojiang

    2015-01-01

    A high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-UV-irradiation (HPLC-QTOF-MS-UV) method was established for rapid separation and structural identification of the constituents in Re Du Ning Injections (RDNI). A total of 20 potentially bioactive compounds including 10 caffeoylquinic acids and 10 iridoid glycosides were identified or tentatively characterized in RDNI by comparing their retention times and MS spectra with those of authentic standards or literature data. In particular, UV-irradiation was employed in the identification of the cis/trans isomers of caffeoylquinic acids. Furthermore, each compound was assigned to the individual raw materials (Artemisia annua L., Lonicera japonica Thunb. or Gardenia jasminoides Ellis) present in RDNI. This is the first time that an HPLC-QTOF-MS-UV analytical method has been used for the identification of caffeoylquinic acids in RDNI. PMID:25265866

  11. 'Genipin' - the natural water soluble cross-linking agent and its importance in the modified drug delivery systems: an overview.

    PubMed

    Manickam, Balamurugan; Sreedharan, Rajesh; Elumalai, Manogaran

    2014-01-01

    One of the popular approaches in controlling drug delivery from the polymeric carriers is suitably achieved by the inclusion of crosslinking agents into the formulations at different concentrations. Nevertheless, addition of the chemical crosslinkers such as glutaraldehyde, formaldehyde etc, used in the drug delivery systems causes very serious cytotoxic reactions. These chemical crosslinking agents did not offer any significant advantageous effects when compared to the natural crosslinking agents for instance genipin, which is quite less toxic, biocompatible and offers very stable crosslinked products. Based on the earlier reports the safety of this particular natural crosslinker is very well established, since it has been widely used as a Chinese traditional medicine for long-time, isolated from fruits of the plant Gardenia jasminoides Ellis. This concise article largely portrayed the value of this unique natural crosslinker, utilized in controlling the drug delivery from the various formulations. PMID:24041312

  12. A genipin-gelatin gel dosimeter for radiation processing

    NASA Astrophysics Data System (ADS)

    Davies, J. B.; Bosi, S. G.; Baldock, C.

    2012-08-01

    Genipin, a fruit extract from Gardenia jasminoides Ellis, forms cross-links in solutions of gelatin, to form a blue hydrogel that bleaches quantitatively upon irradiation and the colour change can be measured with a spectrophotometer. With the addition of sulphuric acid this dosimeter is sufficiently sensitive for quality assurance of radiotherapy level dosimetry. Without sulphuric acid the gel has a reduced sensitivity and responds linearly with dose between 100 and 1000 Gy, making it potentially useful as a dosimeter for radiation processing applications such as the phytosanitary irradiation treatment of food. We investigated the dose response characteristics of this new formulation and found that the darker gels are more sensitive to dose and have a reduced uncertainty.

  13. Preventive effect of crocin in inflamed animals and in LPS-challenged RAW 264.7 cells.

    PubMed

    Xu, Guang-Lin; Li, Guang; Ma, Hua-Ping; Zhong, Hai; Liu, Fei; Ao, Gui-Zhen

    2009-09-23

    Gardenia jasminoides Ellis and Crocus sativus L. are both traditional Chinese medicines that have significant biologic activities on inflammatory processes. But the active ingredients remain unclear. Crocin, a representative of carotenoid compounds, has now drawn considerable attention not only because it is a natural food colorant but also because it has great potential in medicine. But until now, the systematic anti-inflammatory effect of crocin has not been well established. In the present study, experiments were carried out to evaluate the anti-inflammatory effects of crocin in vitro and in vivo. In vitro, cyclooxygenase (COX) inhibition assays showed that crocin exhibits a dual inhibitory activity against the COX-1 and COX-2 enzymes. Anti-inflammatory activity in vivo was evaluated using two animal edema model tests. Pretreatment with crocin (p.o.) dose-dependently inhibited the xylene-induced ear edema in mice and carrageenan-induced paw edema in rats. In gastric lesion tests, crocin was gastric-sparing in that it elicited markedly fewer stomach lesions as compared to the number of stomach lesions caused by indomethacin in rats. In further studies, crocin was found to significantly inhibit the productions of prostaglandin E(2) (PGE(2)) in lipopolysaccharide (LPS)-challenged RAW 264.7, which is parallel to its prevention of the nuclear translocation of the NF-kappaB p50 and p65 subunits. These data indicate that crocin exhibits obvious anti-inflammatory effects and may be one of the active ingredients in Gardenia jasminoides Ellis or Crocus sativus L. that can modulate inflammatory processes. PMID:19754168

  14. CHARACTERIZATION OF CASEINS CROSSLINKED BY A NATURALLY OCCURRING CROSSLINKING AGENT-GENIPIN

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Increasing economic and environmental pressures demand more effective utilization of natural resources. Genipin, a naturally occurring crosslinking agent obtained from the fruit of Gardenia jasminoides, has recently attracted much attention due to its low cytotoxicity compared to traditional crossli...

  15. Novel and recurrent EVC and EVC2 mutations in Ellis-van Creveld syndrome and Weyers acrofacial dyostosis.

    PubMed

    D'Asdia, Maria Cecilia; Torrente, Isabella; Consoli, Federica; Ferese, Rosangela; Magliozzi, Monia; Bernardini, Laura; Guida, Valentina; Digilio, Maria Cristina; Marino, Bruno; Dallapiccola, Bruno; De Luca, Alessandro

    2013-02-01

    Ellis van Creveld syndrome and Weyers acrofacial dysostosis are allelic disorders caused by mutations in EVC or EVC2 genes. We illustrate the results of direct analysis of whole EVC and EVC2 genes' coding regions in 32 unrelated families with clinical diagnosis of Ellis van Creveld syndrome and in 2 families with Weyers acrofacial dysostosis. We identified mutations in 27/32 (84%) cases with Ellis van Creveld syndrome and 2/2 cases with Weyers acrofacial dysostosis. Of the Ellis van Creveld syndrome cases, 20/27 (74%) had a mutation in EVC and 7/27 (26%) in EVC2 genes. The two subjects with Weyers acrofacial dysostosis had a heterozygous mutation in the last exon of EVC2. In total, we detected 25 independent EVC and 11 independent EVC2 mutations. Nineteen EVC mutations (19/25, 76%) and 4 EVC2 mutations (4/11, 36%) were novel. Also one EVC2 gene mutation found in Weyers acrofacial dysostosis was novel. In 5 unrelated cases with a clinical diagnosis of Ellis van Creveld syndrome, we did not find any mutation in either EVC or EVC2 genes. Current findings expand the Ellis van Creveld syndrome and Weyers acrofacial dysostosis mutation spectra, and provide further evidence that the last exon of EVC2 gene is a hot spot for Weyers acrofacial dysostosis mutations. Accordingly, EVC2 exon 22 should be analyzed with priority by mutation screening in individuals with a suspected diagnosis of Weyers acrofacial dysostosis.

  16. The gene for the Ellis-van Creveld syndrome is located on chromosome 4p16.

    PubMed

    Polymeropoulos, M H; Ide, S E; Wright, M; Goodship, J; Weissenbach, J; Pyeritz, R E; Da Silva, E O; Ortiz De Luna, R I; Francomano, C A

    1996-07-01

    Ellis-van Creveld syndrome (EVC) is an autosomal recessive disorder characterized by disproportionate dwarfism, polydactyly, and congenital heart disease. This rare disorder is found with increased frequency among the Old Order Amish community in Lancaster County, Pennsylvania. We have used linkage analysis to localize the gene responsible for the EVC phenotype in nine interrelated Amish pedigrees and three unrelated families from Mexico, Ecuador, and Brazil. We now report the linkage for the Ellis-van Creveld syndrome gene to markers on the distal short arm of human chromosome 4, with Zmax = 6.91 at theta = 0.02 for marker HOX7, in a region proximal to the FGFR3 gene responsible for the achondroplasia phenotype. PMID:8661097

  17. Search for astrophysical rotating Ellis wormholes with x-ray reflection spectroscopy

    NASA Astrophysics Data System (ADS)

    Zhou, Menglei; Cardenas-Avendano, Alejandro; Bambi, Cosimo; Kleihaus, Burkhard; Kunz, Jutta

    2016-07-01

    Recently, two of us have found numerically rotating Ellis wormholes as solutions of four-dimensional Einstein gravity coupled to a phantom field. In this paper, we investigate possible observational signatures to identify similar objects in the Universe. These symmetric wormholes have a mass and are compact, so they may look like black holes. We study the iron line profile in the x-ray reflection spectrum of a thin accretion disk around rotating Ellis wormholes and we find some specific observational signatures that can be used to distinguish these objects from Kerr black holes. We simulate some observations with XIS/Suzaku assuming typical parameters for a bright active galactic nucleus and we conclude that current x-ray missions cannot apply strong constraints.

  18. Prosthodontic Rehabilitation for a Patient with Ellis-Van Creveld Syndrome: A Case Report.

    PubMed

    Siddiqui, Muhammad Musaab; Taylor, Philip D

    2016-03-01

    Patients suffering from Ellis-Van-Creveld syndrome are a challenge for dental management. Aesthetics are a major concern with limited manual dexterity, making choice of treatment critical. A 38 year old female diagnosed with Ellis-Van-Creveld syndrome presented with stained teeth and un-aesthetic smile and related that to her low self-esteem and depression. Intra-oral examination revealed mal-aligned megadont central incisors in the maxillary arch, fused mandibular canines with laterals and missing central incisors with space discrepancy and pronounced reverse over jet and overbite. Treatment involved non-surgical periodontal management, fabrication of veneers and dentine bonded crowns for maxillary anteriors and fixed-fixed resin retained bridge for mandibular arch. PMID:27039477

  19. [From gene to disease; EVC, EVC2, and Ellis-van Creveld syndrome].

    PubMed

    van Hagen, J M; Baart, J A; Gille, J J P

    2005-04-23

    Ellis-van Creveld syndrome is an autosomal recessive disorder characterised by short stature with short limbs, postaxial polydactyly and congenital cardiac defects. The syndrome can be caused by mutations in the EVC gene or the EVC2 gene. The genes are located close to each other in a head-to-head configuration on chromosome 4p16. Clinical diagnosis can be confirmed by DNA analysis, which is currently offered by two laboratories in Italy.

  20. The Frank Ellis memorial lecture: the use of three-dimensional imaging in gynaecological radiation therapy.

    PubMed

    Viswanathan, A N

    2008-02-01

    The use of three-dimensional image guidance in radiation therapy has increased dramatically over the past decade. In gynaecological malignancies, three-dimensional image guidance assists with both external beam and brachytherapy treatment planning, increasing the accuracy of dose delivery. During his lifetime, Frank Ellis made significant contributions to gynaecological brachytherapy. This lecture will focus on novel advances in three-dimensional image-guided radiation therapy for cervical cancer, with the ultimate goal of improving outcomes for our patients.

  1. Taking prisoners: Havelock Ellis, Sigmund Freud, and the construction of homosexuality, 1897-1951.

    PubMed

    Crozier, I D

    2000-12-01

    This paper addresses the efforts of both Havelock Ellis and Sigmund Freud to posit a theory of homosexuality, and especially considers their efforts to (re-)negotiate each other's theories. Its central premise derives from the sociology of scientific knowledge: that it is not what is written, but the way that what is written is treated by ensuing experts, that makes knowledge. In the case study used in this paper, Ellis and Freud struggle to posit what they consider to be the proper model for understanding homosexual desire. They utilize aspects of each other's word, but are careful not to appear to be following each other too closely. Such a struggle to establish different schools of thought is exemplified by the informal negotiations engaged in when a student, Joseph Wortis, made contact with both Freud and Ellis. Again following sociology of scientific knowledge precepts, these informal negotiations (contained in published and archival letters) are used to show how knowledge claims are constructed, deconstructed any reconstructed by the actors who have stakes in the outcome of what is to be regarded as knowledge in the relevant communities.

  2. DNA topoisomerase IIα inhibitory and anti-HIV-1 flavones from leaves and twigs of Gardenia carinata.

    PubMed

    Kongkum, Naowarat; Tuchinda, Patoomratana; Pohmakotr, Manat; Reutrakul, Vichai; Piyachaturawat, Pawinee; Jariyawat, Surawat; Suksen, Kanoknetr; Yoosook, Chalobon; Kasisit, Jitra; Napaswad, Chanita

    2012-03-01

    Four new flavones, 5,2'-dihydroxy-7,3',4',5'-tetramethoxyflavone (1), 5,2',5'-trihydroxy-7,3',4'-trimethoxyflavone (2), 5,7,2',5'-tetrahydroxy-6,3',4'-trimethoxyflavone (3) and 5,2',5'-trihydroxy-6,7,3',4'-tetramethoxyflavone (4), along with the known 5,3'-dihydroxy-6,7,4',5'-tetramethoxyflavone (5), 5,7,3',5'-tetrahydroxy-6,4'-dimethoxyflavone (6), syringaldehyde, vanillic acid and scopoletin were isolated from the leaves and twigs of Gardenia carinata (Rubiaceae). Their structures were determined by spectroscopic methods. Flavone 2 exhibited cytotoxic activity against P-388 and MCF-7 cell lines, while 3, 5 and 6 were active only in P-388 cell line. All active compounds were found to inhibit DNA topoisomerase IIα activity, which may be responsible for the observed cytotoxicity. Flavones 1-3, 5 and 6 also exhibited anti-HIV-1 activity in the anti-syncytium assay using (∆Tat/rev)MC99 virus and 1A2 cell line system; 2 was most potent. Only flavones 1 and 6 showed considerably activity against HIV-1 reverse transcriptase.

  3. Venus Flytrap (Dionaea muscipula Solander ex Ellis) Contains Powerful Compounds that Prevent and Cure Cancer.

    PubMed

    Gaascht, François; Dicato, Mario; Diederich, Marc

    2013-01-01

    Chemoprevention uses natural or synthetic molecules without toxic effects to prevent and/or block emergence and development of diseases including cancer. Many of these natural molecules modulate mitogenic signals involved in cell survival, apoptosis, cell cycle regulation, angiogenesis, or on processes involved in the development of metastases occur naturally, especially in fruits and vegetables bur also in non-comestible plants. Carnivorous plants including the Venus flytrap (Dionaea muscipula Solander ex Ellis) are much less investigated, but appear to contain a wealth of potent bioactive secondary metabolites. Aim of this review is to give insight into molecular mechanisms triggered by compounds isolated from these interesting plants with either therapeutic or chemopreventive potential.

  4. Report of Two Siblings with Overlapping Features of Ellis-van Creveld and Weyers Acrodental Dysostosis

    PubMed Central

    Shetty, Devi C.; Singh, Harkanwal P.; Kumar, Prince; Verma, Chanchal

    2012-01-01

    Skeletal dysplasias are a heterogenous group of disorders combining abnormalities in the skull and other skeletal bones. Weyers acrofacial dysostosis also known as Weyers acrodental dysostosis was first described in 1952, by Weyers, as a postaxial polydactyly, which had features distinct from, yet some in common with the Ellis-van Creveld Syndrome (EvC). Both the syndromes have been mapped to the same chromosome, 4p16. The cases reported here highlight the overlapping features of both syndromes, which are dissimilar in mode of inheritance and phenotypic severity, emphasizing the need for genetic analysis, to categorize these conditions. PMID:22616035

  5. Ellis-van Creveld syndrome with unusual oral and dental findings: A rare clinical entity.

    PubMed

    Shaik, Sameeulla; Raviraj, Jayam; Dirasantchu, Suresh; Venkata, Suman S

    2016-01-01

    Ellis-van Creveld (EVC) syndrome, a form of skeletal and chondroectodermal dysplasia, is an autosomal recessive disorder characterized by a tetrad of disproportionate dwarfism, postaxial polydactyly, ectodermal dysplasia, and heart defects. In the present article, we hereby present a case of a 13-year-old girl of Indian ethnicity with EVC syndrome with a remarkable number of classical oral and dental features, with unusual findings such as taurodontism and talons cusp. Such dental findings were reported in few cases only. Despite the fact that oral manifestations play an important role in the diagnosis of EVC, only a few detailed reports have been published in the dental literature. PMID:27076836

  6. A rare case report of Ellis Van Creveld syndrome in an Indian patient and literature review

    PubMed Central

    Shetty, Prashanth; Shetty, Deepthi; Priyadarshana, P.S.; Bhat, Smitha

    2015-01-01

    Ellis Van Creveld syndrome (EVC) is a rare genetic disorder having autosomal recessive inheritance affecting the Amish population of Pennsylvania in USA with incidence of 1:244,000 for the general population. This syndrome consists of characteristic features such as bilateral postaxial polydactyly, chondroectodermal dysplasia, congenital heart defects and hypoplastic nails and teeth. There are few case reports of this syndrome reported in dental literature. We report a case of a 17 year old female presenting typical features of this syndrome and the oral findings of this patient which are the key diagnostic features. PMID:26258022

  7. Ellis-van Creveld syndrome in adulthood: extending the clinical spectrum.

    PubMed

    Pérez-Andreu, Joaquín; Ray, Victor Glenn; Arribas, José María; Sánchez, Sergio Juan

    2015-06-01

    Ellis-van Creveld (EvC) syndrome is a rare autosomal recessive malformation disorder. Cardiac defects are observed in about 50% of EvC cases. Surgical data is lacking on the prognosis and life expectancy of EvC patients. Herein, we report the case of a 38-year-old man with EvC syndrome who underwent two surgical corrections for cardiac anomalies. This report supplements the available information on the clinical course of EvC syndrome in older patients.

  8. Ellis-van Creveld syndrome with unusual oral and dental findings: A rare clinical entity.

    PubMed

    Shaik, Sameeulla; Raviraj, Jayam; Dirasantchu, Suresh; Venkata, Suman S

    2016-01-01

    Ellis-van Creveld (EVC) syndrome, a form of skeletal and chondroectodermal dysplasia, is an autosomal recessive disorder characterized by a tetrad of disproportionate dwarfism, postaxial polydactyly, ectodermal dysplasia, and heart defects. In the present article, we hereby present a case of a 13-year-old girl of Indian ethnicity with EVC syndrome with a remarkable number of classical oral and dental features, with unusual findings such as taurodontism and talons cusp. Such dental findings were reported in few cases only. Despite the fact that oral manifestations play an important role in the diagnosis of EVC, only a few detailed reports have been published in the dental literature.

  9. Expression of the Ellis-van Creveld (Evc) gene in the rat tibial growth plate.

    PubMed

    Tsuji, Takehito; Nakamura, Hiroaki; Hirata, Azumi; Yamamoto, Toshio

    2004-08-01

    Ellis-van Creveld (EvC) syndrome is an autosomal recessive chondrodysplasia characterized by short limbs, postaxial polydactyly, natal teeth, and dysplastic nails. The Ellis-van Creveld (EVC) gene, which is mutated in patients with EvC syndrome, has been identified by positional cloning. However, the physiological roles of the EVC gene have not been elucidated. Histopathological analyses of EvC syndrome have shown disturbed chondrocytic phenotypes during cartilage development. We therefore postulated that the EVC gene is a critical factor for chondrocytes during endochondral ossification. The present study focuses on the relationship between the Evc gene and chondrocytes, and examines Evc gene expression in the rat tibial growth plate at the mRNA and protein levels. Evc mRNA in tibial epiphyseal cartilage was expressed at postnatal day (P) 1, P28, and P56 by RT-PCR. Immunohistochemical analyses localized the Evc protein mainly in prehypertrophic and hypertrophic chondrocytes of the epiphyseal growth plate in the tibia during the embryonic and postnatal periods. Evc mRNA was also detected in prehypertrophic and hypertrophic chondrocytes by in situ hybridization. These results indicate that the Evc gene functions mainly in the prehypertrophic and hypertrophic chondrocytes of the epiphyseal growth plate. The data presented here are important for future studies of the underlying mechanism of chondrodysplasia in EvC syndrome.

  10. Six seconds per eyelid: the medical inspection of immigrants at Ellis Island 1892-1914.

    PubMed

    Birn, A E

    1997-01-01

    Beginning in 1892, immigrants to the United States were subject to a medical inspection, created to restrict the entry of persons with a"loathsome or dangerous contagious" disease or mental defficiency. Ellis Island, which received over 10 million newcomers between 1900 and 1914, served as the largest ever medical screening facility. Far from reflecting a unified policy, the medical inspection offered a complicated compromise amidst a swirl of competing interests. Many industrialists blamed the waves of Southern and Eastern European immigrants for urban joblessness, filth, unrest, overcrowding, and disease. In an era of depression, labor groups opposed immigrant competitors for scarce jobs. Nativists believed immigrants could not overcome their defects because these were genetically transmitted. Germ theory proponents recognized communication of microorganisms as the problem, with controlling the spread of infections as the solution. Many Progressive reformers held that the scientific screening of immigrants offered a systematic solution for the disorder. Dozens of immigrant aid societies struggled to attenuate the effects of the inspection, and as depression subsided after 1900, employers, too, favored the influx of immigrants. This paper examines the social and political basis for the inspection, its realization at Ellis Island, and the reasons for its inability to debar large numbers of immigrants.

  11. Geniposide Suppresses Hepatic Glucose Production via AMPK in HepG2 Cells.

    PubMed

    Guo, Lixia; Zheng, Xuxu; Liu, Jianhui; Yin, Zhongyi

    2016-01-01

    Geniposide is one of the main compounds in Gardenia jasminoides ELLIS and has many pharmacological activities, but its anti-hyperglycemic activity has not yet been fully explored. This study was designed to determine, for the first time, how geniposide from G. jasminoides regulates hepatic glucose production, and the underlying mechanisms. During in vitro study, we found the inhibitory effect of geniposide on the hepatic glucose production is partly through AMP-activated protein kinase (AMPK) activation in HepG2 cells. Geniposide significantly inhibited hepatic glucose production in a dose-dependent manner. AMPK, acetyl coenzyme A synthetase (ACC) and forkhead box class O1 (FoxO1) phosphorylation were stimulated by different concentrations of geniposide. In addition, the enzyme activities of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) were all significantly suppressed. What is important is that these effects were partly reversed by (1) inhibition of AMPK activity by compound C, a selective AMPK inhibitor, and by (2) suppression of AMPKα expression by small interfering RNA (siRNA). In summary, geniposide potentially ameliorates hyperglycemia through inhibition of hepatic gluconeogenesis by modulation of the AMPK-FoxO1 signaling pathway. Geniposide or geniposide-containing medicinal plants could represent a promising therapeutic agent to prevent type 2 diabetes on gluconeogenesis. PMID:26830672

  12. Training Preachers in the Ellis County Baptist Missionary Association of Texas in the Basics of Expository Preaching

    ERIC Educational Resources Information Center

    Gibson, Timothy Darryle

    2011-01-01

    This project was designed to train active and potential preachers in the Ellis County Baptist Missionary Association of Texas in the basics of expository preaching. Chapter 1 outlines the ministry context at Heritage Baptist Church, the project's purpose, and the project's goals. Chapter 2 examines the biblical and theological rationale that…

  13. Psychological Chauvinism and Nuclear Holocaust: A Response to Albert Ellis and Defense of Non-Rational Emotive Therapies.

    ERIC Educational Resources Information Center

    Walsh, Roger

    1989-01-01

    Claims Ellis' 1986 article on psychology and nuclear holocaust did not address certain key issues and made inaccurate criticisms of non-rational emotive therapies. Attempts to correct these errors and to emphasize the importance of open-minded cooperation among psychologists of differing theoretical persuasions. (Author)

  14. Crocetin, a carotenoid derivative, inhibits VEGF-induced angiogenesis via suppression of p38 phosphorylation.

    PubMed

    Umigai, Naofumi; Tanaka, Junji; Tsuruma, Kazuhiro; Shimazawa, Masamitsu; Hara, Hideaki

    2012-05-01

    We evaluated the protective effects of crocetin against angiogenesis induced by vascular endothelial growth factor (VEGF). Crocetin, the aglycone of crocin carotenoids, is found in saffron crocus (Crocus sativus L.) and gardenia fruit (Gardenia jasminoides Ellis). The effects of crocetin on VEGF-induced angiogenesis were examined by in vitro tube formation assays and following 14-day co-culture of human umbilical vein endothelial cells (HUVECs) and fibroblasts. The anti-angiogenic mechanism of crocetin was evaluated by examining its effects on VEGF-induced proliferation and migration of human retinal microvascular endothelial cells (HRMECs) and phosphorylation of p38. Vascular endothelial (VE)-cadherin, zonula occludens (ZO-1) and occludin, which are adherens and tight junction proteins, respectively, play a major role in the control of vascular permeability. Therefore, we tested effects of crocetin on adhesion molecule dissociation induced by VEGF. Crocetin significantly suppressed VEGF-induced tube formation by HUVECs and migration of HRMECs. It also significantly inhibited phosphorylation of p38 and protected VE-cadherin expression. These findings indicate that crocetin suppresses the VEGF-induced angiogenesis by inhibiting migration and that the inhibition of phosphorylated-p38 and protection of VE-cadherin expression may be involved in its underlying mechanism of action. PMID:22475394

  15. Insight of Saffron Proteome by Gel-Electrophoresis.

    PubMed

    Paredi, Gianluca; Raboni, Samanta; Marchesani, Francesco; Ordoudi, Stella A; Tsimidou, Maria Z; Mozzarelli, Andrea

    2016-01-01

    Saffron is a spice comprised of the dried stigmas and styles of Crocus sativus L. flowers and, since it is very expensive, it is frequently adulterated. So far, proteomic tools have never been applied to characterize the proteome of saffron or identify possible cases of fraud. In this study, 1D-Gel Electrophoresis was carried out to characterize the protein profile of (i) fresh stigmas and styles of the plant; (ii) dried stigmas and styles from different geographical origins (Spanish, Italian, Greek and Iranian) that had been stored for various periods of time after their processing; and (iii) two common plant adulterants, dried petals of Carthamus tinctorius L. and dried fruits of Gardenia jasminoides Ellis. A selective protein extraction protocol was applied to avoid interference from colored saffron metabolites, such as crocins, during electrophoretic analyses of saffron. We succeeded in separating and assigning the molecular weights to more than 20 proteins. In spite of the unavailability of the genome of saffron, we were able to identify five proteins by Peptide Mass Fingerprinting: phosphoenolpyruvate carboxylase 3, heat shock cognate 70 KDa protein, crocetin glucosyltransferase 2, α-1,4-glucan-protein synthase and glyceraldehydes-3-phosphate dehydrogenase-2. Our findings indicate that (i) few bands are present in all saffron samples independently of origin and storage time, with amounts that significantly vary among samples and (ii) aging during saffron storage is associated with a reduction in the number of detectable bands, suggesting that proteases are still active. The protein pattern of saffron was quite distinct from those of two common adulterants, such as the dried petals of Carthamus tinctorius and the dried fruits of Gardenia jasminoides indicating that proteomic analyses could be exploited for detecting possible frauds. PMID:26840283

  16. Insight of Saffron Proteome by Gel-Electrophoresis.

    PubMed

    Paredi, Gianluca; Raboni, Samanta; Marchesani, Francesco; Ordoudi, Stella A; Tsimidou, Maria Z; Mozzarelli, Andrea

    2016-01-29

    Saffron is a spice comprised of the dried stigmas and styles of Crocus sativus L. flowers and, since it is very expensive, it is frequently adulterated. So far, proteomic tools have never been applied to characterize the proteome of saffron or identify possible cases of fraud. In this study, 1D-Gel Electrophoresis was carried out to characterize the protein profile of (i) fresh stigmas and styles of the plant; (ii) dried stigmas and styles from different geographical origins (Spanish, Italian, Greek and Iranian) that had been stored for various periods of time after their processing; and (iii) two common plant adulterants, dried petals of Carthamus tinctorius L. and dried fruits of Gardenia jasminoides Ellis. A selective protein extraction protocol was applied to avoid interference from colored saffron metabolites, such as crocins, during electrophoretic analyses of saffron. We succeeded in separating and assigning the molecular weights to more than 20 proteins. In spite of the unavailability of the genome of saffron, we were able to identify five proteins by Peptide Mass Fingerprinting: phosphoenolpyruvate carboxylase 3, heat shock cognate 70 KDa protein, crocetin glucosyltransferase 2, α-1,4-glucan-protein synthase and glyceraldehydes-3-phosphate dehydrogenase-2. Our findings indicate that (i) few bands are present in all saffron samples independently of origin and storage time, with amounts that significantly vary among samples and (ii) aging during saffron storage is associated with a reduction in the number of detectable bands, suggesting that proteases are still active. The protein pattern of saffron was quite distinct from those of two common adulterants, such as the dried petals of Carthamus tinctorius and the dried fruits of Gardenia jasminoides indicating that proteomic analyses could be exploited for detecting possible frauds.

  17. The gene for the Ellis-van Creveld syndrome is located on chromosome 4p16

    SciTech Connect

    Polymeropoulos, M.H.; Ide, S.E.; Wright, M.

    1996-07-01

    Ellis-van Creveld syndrome (EVC) is an autosomal recessive disorder characterized by disproportionate dwarfism, polydactyly, and congenital heart disease. This rare disorder is found with increased frequency among the Old Order Amish community in Lancaster County, Pennsylvania. We have used linkage analysis to localize the gene responsible for the EVC phenotype in nine interrelated Amish pedigrees and three unrelated families from Mexico, Ecuador, and Brazil. We now report the linkage for the Ellisvan Creveld syndrome gene to markers on the distal short arm of human chromosome 4, with Z{sub max} = 6.91 at {theta} = 0.02 for marker HOX7, in a region proximal to the FGFR3 gene responsible for the achondroplasia phenotype. 17 refs., 2 figs., 1 tab.

  18. Venus Flytrap (Dionaea muscipula Solander ex Ellis) Contains Powerful Compounds that Prevent and Cure Cancer

    PubMed Central

    Gaascht, François; Dicato, Mario; Diederich, Marc

    2013-01-01

    Chemoprevention uses natural or synthetic molecules without toxic effects to prevent and/or block emergence and development of diseases including cancer. Many of these natural molecules modulate mitogenic signals involved in cell survival, apoptosis, cell cycle regulation, angiogenesis, or on processes involved in the development of metastases occur naturally, especially in fruits and vegetables bur also in non-comestible plants. Carnivorous plants including the Venus flytrap (Dionaea muscipula Solander ex Ellis) are much less investigated, but appear to contain a wealth of potent bioactive secondary metabolites. Aim of this review is to give insight into molecular mechanisms triggered by compounds isolated from these interesting plants with either therapeutic or chemopreventive potential. PMID:23971004

  19. Stability analysis of a Morris-Thorne-Bronnikov-Ellis wormhole with pressure

    SciTech Connect

    Novikov, I. D. Shatskiy, A. A.

    2012-05-15

    The model of a spherical Morris-Thorne-Bronnikov-Ellis wormhole is analyzed for stability. The matter of this wormhole is composed of a radial monopole magnetic field and a quasi-perfect phantom fluid. In the stationary case, the energy density of this fluid is negative and equal in magnitude to twice the energy density of the magnetic field. There is no pressure of this fluid in the stationary case (phantom dust), while in the case where the fluid energy density deviates from its stationary value, the pressure is proportional to the deviation of the energy density from its stationary value. An example of a wormhole stable against radial perturbations has been obtained.

  20. Extracting and purifying R-phycoerythrin from Mediterranean red algae Corallina elongata Ellis & Solander.

    PubMed

    Rossano, R; Ungaro, N; D'Ambrosio, A; Liuzzi, G M; Riccio, P

    2003-03-20

    R-Phycoerythrin (R-PE) is a protein acting as a photosynthetic accessory pigment in red algae (Rodophyta). This protein has gained importance in many biotechnological applications in food science, immunodiagnostic, therapy, cosmetics, protein and cell labelling, and analytical processes. In this paper we report on a new, one step procedure for the extraction and purification of R-PE from a new source: the Mediterranean red algae Corallina elongata Ellis & Solander. This red algae contains mainly R-PE and is suitable for the production in culture. No other contaminating phycobiliproteins could be detected in the extracts. The method we propose for the purification is based on the use of hydroxyapatite, a chromatographic resin that can be produced in the laboratory at very low cost and can be used batch-wise with large amounts of extracts, alternative to chromatography, and therefore can be scaled up. Both the yield and the purity of R-PE are very good.

  1. Phosphorus loading to McGrath and Ellis ponds, Kennebec County, Maine

    USGS Publications Warehouse

    Nichols, Wallace J.; Sowles, J.W.; Lobao, J.J.

    1984-01-01

    McGrath and Ellis Ponds in south-central Maine have been identified as having nuisance algae blooms. In 1978, a cooperative study between the U.S. Geological Survey and the Maine Department Environmental Protection was begun to evaluate areas in which restoration effort would best improve water quality of the ponds. Streamflow and phosphorus data were collected from 28 tributaries to the ponds, April 1 through September 30, 1978 and 1979. Phosphorus yields from each tributary watershed were compared to determine their relative importance to the phosphorus budgets of the ponds. Three tributaries to the ponds were estimated to contribute 44 percent of the phosphorus load, yet drain only 22 percent of the watershed. Phosphorus input to the ponds likely would be most easily reduced by instituting phosphorus control practices in parts of the basin drained by the three tributaries. (USGS)

  2. [Photosensitization in cattle grazing on pastures of Brahciaria decumbens Stapf infested with Pithomyces chartarum (Berk. & Curt.) M.B. Ellis].

    PubMed

    Andrade, S O; da Silva Lopes, H O; de Almeida Barros, M; Leite, G G; Dias, S M; Saueressig, M; Nobre, D; Temperini, J A

    1978-01-01

    Aspects of photosensitization in bovines grazing on pastures of Brachiaria decumbens Stapf infested with Pithomyces chartarum (Berk. & Curt.) M.B. Ellis infested all pastures 45(2):117-136, 1978. This paper reports experimental studies on photosensitization in bovines grazing on different pastures of Brachiaria decumbens Stapf in the "Cerrados" region (Planaltina, DF). Climatic conditions, zinc content and occurence of fungi on pastures were investigated. Pithomyces chartarum (Berk. & Curt.) M.B. Ellis infested all pastures examined. Photosensitization was observed in one animal maintained on a pasture of B. decumbens formed with seeds from Australia. Clinical and necropsy data were similar to those related in literature for sporidesmin-intoxicated animals. An isolate of P. chartarum and samples of bovine bile were assayed for sporidesmin presence.

  3. [Photosensitization in cattle grazing on pastures of Brahciaria decumbens Stapf infested with Pithomyces chartarum (Berk. & Curt.) M.B. Ellis].

    PubMed

    Andrade, S O; da Silva Lopes, H O; de Almeida Barros, M; Leite, G G; Dias, S M; Saueressig, M; Nobre, D; Temperini, J A

    1978-01-01

    Aspects of photosensitization in bovines grazing on pastures of Brachiaria decumbens Stapf infested with Pithomyces chartarum (Berk. & Curt.) M.B. Ellis infested all pastures 45(2):117-136, 1978. This paper reports experimental studies on photosensitization in bovines grazing on different pastures of Brachiaria decumbens Stapf in the "Cerrados" region (Planaltina, DF). Climatic conditions, zinc content and occurence of fungi on pastures were investigated. Pithomyces chartarum (Berk. & Curt.) M.B. Ellis infested all pastures examined. Photosensitization was observed in one animal maintained on a pasture of B. decumbens formed with seeds from Australia. Clinical and necropsy data were similar to those related in literature for sporidesmin-intoxicated animals. An isolate of P. chartarum and samples of bovine bile were assayed for sporidesmin presence. PMID:573108

  4. Active movements in plants: Mechanism of trap closure by Dionaea muscipula Ellis.

    PubMed

    Markin, Vladislav S; Volkov, Alexander G; Jovanov, Emil

    2008-10-01

    The Venus flytrap (Dionaea muscipula Ellis) captures insects with one of the most rapid movements in the plant kingdom. We investigated trap closure by mechanical and electrical stimuli using the novel charge-injection method and high-speed recording. We proposed a new hydroelastic curvature mechanism, which is based on the assumption that the lobes possess curvature elasticity and are composed of outer and inner hydraulic layers with different hydrostatic pressure. The open state of the trap contains high elastic energy accumulated due to the hydrostatic pressure difference between the hydraulic layers of the lobe. Stimuli open pores connecting the two layers, water rushes from one hydraulic layer to another, and the trap relaxes to the equilibrium configuration corresponding to the closed state. In this paper we derived equations describing this system based on elasticity Hamiltonian and found closing kinetics. The novel charge-injection stimulation method gives insight into mechanisms of the different steps of signal transduction and response in the plant kingdom.

  5. Morphing structures of the Dionaea muscipula Ellis during the trap opening and closing

    PubMed Central

    Volkov, Alexander G; Forde-Tuckett, Victoria; Volkova, Maya I; Markin, Vladislav S

    2014-01-01

    The Venus flytrap is a marvelous plant that has intrigued scientists since the times of Charles Darwin. This carnivorous plant is capable of very fast movements to catch a prey. We found that the maximal speed of the trap closing in the Dionaea muscipula Ellis is about 130 000 times faster than the maximal speed of the trap opening. The mechanism and kinetics of this movement was debated for a long time. Here, the most recent Hydroelastic Curvature Model is applied to the analysis of this movement during closing and opening of the trap with or without a prey. Equations describing the trap movement were derived and verified with experimental data. Chloroform and ether, both anesthetic agents, induce action potentials and close the trap without the mechanical stimulation of trigger hairs. We tested this by dropping 10 μL of ether on the midrib inside the trap without touching any of the mechanosensitive trigger hairs. The trap closed slowly in 10 s. This is at least 20 times slower than the closing of the trap mechanically or electrically. The similar effect can be induced by placing 10 μL of chloroform on the midrib inside the trap, however, the lobes closing time in this case is as fast as closing after mechanical stimulation of the trigger hairs. PMID:24618927

  6. Morphing structures of the Dionaea muscipula Ellis during the trap opening and closing.

    PubMed

    Volkov, Alexander G; Forde-Tuckett, Victoria; Volkova, Maya I; Markin, Vladislav S

    2014-01-01

    The Venus flytrap is a marvelous plant that has intrigued scientists since the times of Charles Darwin. This carnivorous plant is capable of very fast movements to catch a prey. We found that the maximal speed of the trap closing in the Dionaea muscipula Ellis is about 130,000 times faster than the maximal speed of the trap opening. The mechanism and kinetics of this movement was debated for a long time. Here, the most recent Hydroelastic Curvature Model is applied to the analysis of this movement during closing and opening of the trap with or without a prey. Equations describing the trap movement were derived and verified with experimental data. Chloroform and ether, both anesthetic agents, induce action potentials and close the trap without the mechanical stimulation of trigger hairs. We tested this by dropping 10 μL of ether on the midrib inside the trap without touching any of the mechanosensitive trigger hairs. The trap closed slowly in 10 s. This is at least 20 times slower than the closing of the trap mechanically or electrically. The similar effect can be induced by placing 10 μL of chloroform on the midrib inside the trap, however, the lobes closing time in this case is as fast as closing after mechanical stimulation of the trigger hairs.

  7. Two Adult Patients with Ellis-van Creveld Syndrome Extending the Clinical Spectrum.

    PubMed

    Rudnik-Schöneborn, S; Zerres, K; Graul-Neumann, L; Wiegand, S; Mellerowicz, H; Hehr, U

    2011-09-01

    Ellis-van Creveld (EvC) syndrome is a rare autosomal recessive malformation syndrome with the main features cardiac defects, postaxial hexadactyly, mesomelic shortening of the limbs, short ribs, dysplastic nails and teeth, oral frenula and various other abnormalities while mental function is normal. We describe 2 adult EvC patients with the cardinal skeletal features of mesomelic short stature and severe, progressive genu valgum deformity, resulting from loss of function mutations in the EVC genes. While the genu valgum was the predominating and disabling feature in patient 1, patient 2 showed acroosteolyses in the distal phalanges and a symmetrical synostosis of metacarpals in his hands. Moreover, patient 2 developed synostoses in the additional fingers in adolescence which had not been present at the age of 12 years, suggesting a further progression of skeletal disease. Joint fusion of phalanges so far has not been reported in EvC syndrome. Our data further expand the phenotypic spectrum of EvC related skeletal malformations and contribute important new information on the clinical course of EvC syndrome with increasing age.

  8. Analysis of Ellis van Creveld syndrome gene products: implications for cardiovascular development and disease.

    PubMed

    Sund, Kristen Lipscomb; Roelker, Stephanie; Ramachandran, Vijaya; Durbin, Lisa; Benson, D Woodrow

    2009-05-15

    Mutations identified in a cohort of patients with atrioventricular septal defects as a part of Ellis van Creveld syndrome (EvC syndrome) led us to study the role of two non-homologous genes, EVC and LBN, in heart development and disease pathogenesis. To address the cause of locus heterogeneity resulting in an indistinguishable heart-hand phenotype, we carried out in situ hybridization and immunofluorescence and identified co-localization of Evc and Lbn mRNA and protein. In the heart, expression was identified to be strongest in the secondary heart field, including both the outflow tract and the dorsal mesenchymal protrusion, but was also found in mesenchymal structures of the atrial septum and the atrioventricular cushions. Finally, we studied the transcriptional hierarchy of EVC and LBN but did not find any evidence of direct transcriptional interregulation between the two. Due to the locus heterogeneity of human mutations predicted to result in a loss of protein function, a bidirectional genomic organization and overlapping expression patterns, we speculate that these proteins function coordinately in cardiac development and that loss of this coordinate function results in the characteristics of EvC syndrome.

  9. Growth hormone analysis and treatment in Ellis-van Creveld syndrome.

    PubMed

    Versteegh, Florens G A; Buma, Sannine A; Costin, Gertrude; de Jong, Wilfried C; Hennekam, Raoul C M

    2007-09-15

    Little is known on growth, growth hormone (GH) levels and GH treatment in patients with Ellis-van Creveld syndrome (EvC). The aim of the present study was to assess growth, growth hormone status and the possible effectiveness of GH treatment in literature and in a small series of EvC patients. A review of literature indicated retarded growth for most EvC patients (-2 to -4.5 SDS) and minimal data on GH levels or treatment which did not allow any conclusion. We studied eight EvC patients, seven of whom were treated with GH. Four were GH deficient (GHD) and four were GH sufficient. In all patients treated with GH, first year growth velocity increased. In three of the four GHD and in one GH-sufficient patient a gain in height SDS was noted. In the present small EvC series GHD occurred more often than expected. Patient acquisition through the Growth Hormone Database will have caused a significant bias, but the present results indicate that GH treatment may improve growth in at least some patients with EvC. Therefore we conclude that EvC patients may benefit from being tested for GHD and, if indicated, treated. In addition a prospective study to evaluate GH status and linear growth in patients with EvC as well as the potential effectiveness of GH treatment is warranted.

  10. Ellis-van Creveld syndrome associated with chronic intestinal pseudo-obstruction.

    PubMed

    Iwakura, Hideo; Fujii, Katsunori; Furutani, Yoshiyuki; Takatani, Tomozumi; Ebata, Ryota; Nakanishi, Toshio; Mitsunaga, Tetsuya; Saito, Takeshi; Kishimoto, Takashi; Yoshida, Hideo; Shimojo, Naoki

    2016-01-01

    Ellis-van Creveld (EVC) syndrome is a rare autosomal recessive disorder characterized by hypoplastic nails, polydactyly, and achondroplasia. Patients usually exhibit normal cognitive function and no remarkable developmental delay. We herein present an unusual case of EVC syndrome. A Japanese 2-year-old boy was born at term, but immediately developed severe respiratory failure due to thorax deformity, postaxial polydactyly and nail hypoplasia. We identified a novel pattern of germinal compound heterozygous nonsense EVC2 mutations of c.1814C > A (p. S605X) and c.2653C > T (p. R885X), leading to the diagnosis of EVC syndrome. Interestingly, he also had severe developmental delay, and suddenly developed excessive abdominal distension at the age of 2. On surgery, extensive necrotic bowel with chronic intestinal pseudo-obstruction was noted. This is, to our knowledge, a most severe phenotype of EVC syndrome, illustrating that the specific pattern of EVC2 compound heterozygous mutations may cause severe developmental delay and intestinal malfunction.

  11. Mutations in a new gene in Ellis-van Creveld syndrome and Weyers acrodental dysostosis.

    PubMed

    Ruiz-Perez, V L; Ide, S E; Strom, T M; Lorenz, B; Wilson, D; Woods, K; King, L; Francomano, C; Freisinger, P; Spranger, S; Marino, B; Dallapiccola, B; Wright, M; Meitinger, T; Polymeropoulos, M H; Goodship, J

    2000-03-01

    Ellis-van Creveld syndrome (EvC, MIM 225500) is an autosomal recessive skeletal dysplasia characterized by short limbs, short ribs, postaxial polydactyly and dysplastic nails and teeth. Congenital cardiac defects, most commonly a defect of primary atrial septation producing a common atrium, occur in 60% of affected individuals. The disease was mapped to chromosome 4p16 in nine Amish subpedigrees and single pedigrees from Mexico, Ecuador and Brazil. Weyers acrodental dysostosis (MIM 193530), an autosomal dominant disorder with a similar but milder phenotype, has been mapped in a single pedigree to an area including the EvC critical region. We have identified a new gene (EVC), encoding a 992-amino-acid protein, that is mutated in individuals with EvC. We identified a splice-donor change in an Amish pedigree and six truncating mutations and a single amino acid deletion in seven pedigrees. The heterozygous carriers of these mutations did not manifest features of EvC. We found two heterozygous missense mutations associated with a phenotype, one in a man with Weyers acrodental dysostosis and another in a father and his daughter, who both have the heart defect characteristic of EvC and polydactyly, but not short stature. We suggest that EvC and Weyers acrodental dysostosis are allelic conditions.

  12. Novel mutations in EVC cause aberrant splicing in Ellis-van Creveld syndrome.

    PubMed

    Shi, Lisong; Luo, Chunyan; Ahmed, Mairaj K; Attaie, Ali B; Ye, Xiaoqian

    2016-04-01

    Ellis-van Creveld syndrome (EvC) is a rare autosomal recessive disorder characterized by disproportionate chondrodysplasia, postaxial polydactyly, nail dystrophy, dental abnormalities and in a proportion of patients, congenital cardiac malformations. Weyers acrofacial dysostosis (Weyers) is another dominantly inherited disorder allelic to EvC syndrome but with milder phenotypes. Both disorders can result from loss-of-function mutations in either EVC or EVC2 gene, and phenotypes associated with the two gene mutations are clinically indistinguishable. We present here a clinical and molecular analysis of a Chinese family manifested specific features of EvC syndrome. Sequencing of both EVC and EVC2 identified two novel heterozygous splice site mutations c.384+5G>C in intron 3 and c.1465-1G>A in intron 10 in EVC, which were inherited from mother and father, respectively. In vitro minigene expression assay, RT-PCR and sequencing analysis demonstrated that c.384+5G>C mutation abolished normal splice site and created a new cryptic acceptor site within exon 4, whereas c.1465-1G>A mutation affected consensus splice junction site and resulted in full exon 11 skipping. These two aberrant pre-mRNA splicing processes both produced in-frame abnormal transcripts that possibly led to abolishment of important functional domains. To our knowledge, this is the first report of EVC mutations that cause EvC syndrome in Chinese population. Our data revealed that EVC splice site mutations altered splicing pattern and helped elucidate the pathogenesis of EvC syndrome. PMID:26621368

  13. Ellis-van Creveld Syndrome in Grey Alpine Cattle: Morphologic, Immunophenotypic, and Molecular Characterization.

    PubMed

    Muscatello, L V; Benazzi, C; Dittmer, K E; Thompson, K G; Murgiano, L; Drögemüller, C; Avallone, G; Gentile, A; Edwards, J F; Piffer, C; Bolcato, M; Brunetti, B

    2015-09-01

    Ellis-van Creveld (EvC) syndrome is a human autosomal recessive disorder caused by a mutation in either the EVC or EVC2 gene, and presents with short limbs, polydactyly, and ectodermal and heart defects. The aim of this study was to understand the pathologic basis by which deletions in the EVC2 gene lead to chondrodysplastic dwarfism and to describe the morphologic, immunohistochemical, and molecular hallmarks of EvC syndrome in cattle. Five Grey Alpine calves, with a known mutation in the EVC2 gene, were autopsied. Immunohistochemistry was performed on bone using antibodies to collagen II, collagen X, sonic hedgehog, fibroblast growth factor 2, and Ki67. Reverse transcription polymerase chain reaction was performed to analyze EVC1 and EVC2 gene expression. Autopsy revealed long bones that were severely reduced in length, as well as genital and heart defects. Collagen II was detected in control calves in the resting, proliferative, and hypertrophic zones and in the primary and secondary spongiosa, with a loss of labeling in the resting zone of 2 dwarfs. Collagen X was expressed in hypertrophic zone in the controls but was absent in the EvC cases. In affected calves and controls, sonic hedgehog labeled hypertrophic chondrocytes and primary and secondary spongiosa similarly. FGF2 was expressed in chondrocytes of all growth plate zones in the control calves but was lost in most EvC cases. The Ki67 index was lower in cases compared with controls. EVC and EVC2 transcripts were detected. Our data suggest that EvC syndrome of Grey Alpine cattle is a disorder of chondrocyte differentiation, with accelerated differentiation and premature hypertrophy of chondrocytes, and could be a spontaneous model for the equivalent human disease.

  14. Novel mutations in EVC cause aberrant splicing in Ellis-van Creveld syndrome.

    PubMed

    Shi, Lisong; Luo, Chunyan; Ahmed, Mairaj K; Attaie, Ali B; Ye, Xiaoqian

    2016-04-01

    Ellis-van Creveld syndrome (EvC) is a rare autosomal recessive disorder characterized by disproportionate chondrodysplasia, postaxial polydactyly, nail dystrophy, dental abnormalities and in a proportion of patients, congenital cardiac malformations. Weyers acrofacial dysostosis (Weyers) is another dominantly inherited disorder allelic to EvC syndrome but with milder phenotypes. Both disorders can result from loss-of-function mutations in either EVC or EVC2 gene, and phenotypes associated with the two gene mutations are clinically indistinguishable. We present here a clinical and molecular analysis of a Chinese family manifested specific features of EvC syndrome. Sequencing of both EVC and EVC2 identified two novel heterozygous splice site mutations c.384+5G>C in intron 3 and c.1465-1G>A in intron 10 in EVC, which were inherited from mother and father, respectively. In vitro minigene expression assay, RT-PCR and sequencing analysis demonstrated that c.384+5G>C mutation abolished normal splice site and created a new cryptic acceptor site within exon 4, whereas c.1465-1G>A mutation affected consensus splice junction site and resulted in full exon 11 skipping. These two aberrant pre-mRNA splicing processes both produced in-frame abnormal transcripts that possibly led to abolishment of important functional domains. To our knowledge, this is the first report of EVC mutations that cause EvC syndrome in Chinese population. Our data revealed that EVC splice site mutations altered splicing pattern and helped elucidate the pathogenesis of EvC syndrome.

  15. Ellis-van Creveld syndrome and congenital heart defects: presentation of an additional 32 cases.

    PubMed

    Hills, Christine B; Kochilas, Lazaros; Schimmenti, Lisa A; Moller, James H

    2011-10-01

    Ellis-van Creveld (EVC) syndrome is a rare genetic abnormality that has been linked to a mutation in the EVC or EVC2 genes. Common atrium (CA) is an uncommon cardiac malformation, and yet it is commonly found in patients with EVC. We performed a retrospective review of the cases submitted to the Pediatric Cardiac Care Consortium (PCCC) between 1982 and 2007. A review of the English-language literature for previously published cases, as well as current genetic research findings, was also performed. Thirty-two pediatric patients with congenital heart disease (CHD) and EVC syndrome were identified in the PCCC database. Twenty-eight (88%) had an endocardial cushion defect, with 15 of these having primary failure of atrial septation resulting in CA. Persistent left superior vena cava (LSVC) and pulmonary venous connection abnormalities were common. The incidence of persistent LSVC and pulmonary venous abnormalities were greater than previously reported for patients with EVC. Our study reviews the reported literature and adds 32 additional cases from the PCCC database. Review of the cardiac phenotype in patients with EVC syndrome reveals a characteristic pattern of atrioventricular canal defects with systemic and pulmonary venous abnormalities. The frequent association of these abnormalities is strongly reminiscent of the cardiac phenotype found in patients with heterotaxy syndromes. Emerging molecular and developmental studies suggest that EVC and EVC2 proteins may be important for cilia function, which is implicated in the pathogenesis of heterotaxy syndromes. It is speculated that coordinate function between the EVC proteins is required for a cilia-dependent cardiac morphogenesis.

  16. Epigenetic deregulation of Ellis Van Creveld confers robust Hedgehog signaling in adult T-cell leukemia.

    PubMed

    Takahashi, Ryutaro; Yamagishi, Makoto; Nakano, Kazumi; Yamochi, Toshiko; Yamochi, Tadanori; Fujikawa, Dai; Nakashima, Makoto; Tanaka, Yuetsu; Uchimaru, Kaoru; Utsunomiya, Atae; Watanabe, Toshiki

    2014-09-01

    One of the hallmarks of cancer, global gene expression alteration, is closely associated with the development and malignant characteristics associated with adult T-cell leukemia (ATL) as well as other cancers. Here, we show that aberrant overexpression of the Ellis Van Creveld (EVC) family is responsible for cellular Hedgehog (HH) activation, which provides the pro-survival ability of ATL cells. Using microarray, quantitative RT-PCR and immunohistochemistry we have demonstrated that EVC is significantly upregulated in ATL and human T-cell leukemia virus type I (HTLV-1)-infected cells. Epigenetic marks, including histone H3 acetylation and Lys4 trimethylation, are specifically accumulated at the EVC locus in ATL samples. The HTLV-1 Tax participates in the coordination of EVC expression in an epigenetic fashion. The treatment of shRNA targeting EVC, as well as the transcription factors for HH signaling, diminishes the HH activation and leads to apoptotic death in ATL cell lines. We also showed that a HH signaling inhibitor, GANT61, induces strong apoptosis in the established ATL cell lines and patient-derived primary ATL cells. Therefore, our data indicate that HH activation is involved in the regulation of leukemic cell survival. The epigenetically deregulated EVC appears to play an important role for HH activation. The possible use of EVC as a specific cell marker and a novel drug target for HTLV-1-infected T-cells is implicated by these findings. The HH inhibitors are suggested as drug candidates for ATL therapy. Our findings also suggest chromatin rearrangement associated with active histone markers in ATL.

  17. Ellis Van Creveld2 is Required for Postnatal Craniofacial Bone Development.

    PubMed

    Badri, Mohammed K; Zhang, Honghao; Ohyama, Yoshio; Venkitapathi, Sundharamani; Kamiya, Nobuhiro; Takeda, Haruko; Ray, Manas; Scott, Greg; Tsuji, Takehito; Kunieda, Tetsuo; Mishina, Yuji; Mochida, Yoshiyuki

    2016-08-01

    Ellis-van Creveld (EvC) syndrome is a genetic disorder with mutations in either EVC or EVC2 gene. Previous case studies reported that EvC patients underwent orthodontic treatment, suggesting the presence of craniofacial bone phenotypes. To investigate whether a mutation in EVC2 gene causes a craniofacial bone phenotype, Evc2 knockout (KO) mice were generated and cephalometric analysis was performed. The heads of wild type (WT), heterozygous (Het) and homozygous Evc2 KO mice (1-, 3-, and 6-week-old) were prepared and cephalometric analysis based on the selected reference points on lateral X-ray radiographs was performed. The linear and angular bone measurements were then calculated, compared between WT, Het and KO and statistically analyzed at each time point. Our data showed that length of craniofacial bones in KO was significantly lowered by ∼20% to that of WT and Het, the growth of certain bones, including nasal bone, palatal length, and premaxilla was more affected in KO, and the reduction in these bone length was more significantly enhanced at later postnatal time points (3 and 6 weeks) than early time point (1 week). Furthermore, bone-to-bone relationship to cranial base and cranial vault in KO was remarkably changed, i.e. cranial vault and nasal bone were depressed and premaxilla and mandible were developed in a more ventral direction. Our study was the first to show the cause-effect relationship between Evc2 deficiency and craniofacial defects in EvC syndrome, demonstrating that Evc2 is required for craniofacial bone development and its deficiency leads to specific facial bone growth defect. Anat Rec, 299:1110-1120, 2016. © 2016 Wiley Periodicals, Inc.

  18. The inaugural Frank Ellis Lecture--latrogenic cancer: the impact of intensity-modulated radiotherapy.

    PubMed

    Hall, E J

    2006-05-01

    It is an honour and personal pleasure to give the inaugural Frank Ellis Lecture to celebrate his 100th birthday, and to acknowledge his enormous contributions to radiation oncology. Intensity-modulated radiotherapy (IMRT) allows dose to be concentrated in the tumour volume while sparing normal tissues. However, the downside to IMRT is the potential to increase the number of radiation-induced second cancers because more fields are used which involves a bigger volume of normal tissue exposed to lower doses. It has been estimated that IMRT may double the incidence of solid cancers in long-term survivors. This may be acceptable in older patients if balanced by an improvement in local tumour control and reduced toxicity. On the other hand, the incidence of second cancers is higher in children, so that doubling it may not be acceptable. IMRT represents a special case for children. First, they are more sensitive to radiation-induced cancer than adults. Second, radiation scattered from the treatment volume is more important in the small body of the child. Third, there is the question of genetic susceptibility, as many childhood cancers involve a germline mutation. The levels of leakage radiation in current Linacs can be reduced, but the cost would be substantial. An alternative strategy is to replace X-rays with protons. This is an advantage only if the proton machine uses a pencil scanning beam, as passive modulation of a scattering foil produces neutrons, which results in an effective dose to the patient higher than that characteristic of IMRT.

  19. Temporal Patterns of Protozooplankton Abundance and Their Food in Ellis Fjord, Princess Elizabeth Land, Eastern Antarctica

    NASA Astrophysics Data System (ADS)

    Grey, J.; Laybourn-Parry, J.; Leakey, R. J. G.; McMinn, A.

    1997-07-01

    The abundance and biomass of ciliates, dinoflagellates and heterotrophic and phototrophic nanoflagellates were determined at three sites along an ice-covered Antarctic fjord between January and November 1993. The water column showed little in the way of temperature and salinity gradients during the study period. In general, the protozooplankton exhibited a seasonal variation which closely mirrored that of chlorophyllaand bacterioplankton. The fjord mouth, which was affected by the greatest marine influences, consistently had the highest densities of ciliates and the most diverse community, with up to 18 species during the sampling period. Small aloricate ciliates were present throughout the year withStrobilidiumspp. being dominant during the winter. Larger loricate and aloricate ciliates became more prominent during January and November, along with the autotrophic ciliateMesodinium rubrumand two mixotrophic species (Strombidium wulffiand a type resemblingTontonia) suggesting evidence of species successions. Data on dinoflagellates were less extensive, but these protists showed greatest species diversity in the middle reaches of the fjord. A total of 13 species of dinoflagellate was recorded. Ciliates made a significant contribution to the biomass of the microbial community in summer, particularly in the middle and at the seaward end of the fjord. In winter, heterotrophic flagellates (HNAN) and phototrophic nanoflagellates (PNAN) were the dominant component of protistan biomass. In terms of percentage contribution to the microbial carbon pool, bacteria dominated during winter and spring. To the authors' knowledge, this is the first seasonal study of an Antarctic fjord. The Ellis Fjord is very unproductive compared to lower latitude systems, and supports low biomass of phytoplankton and microbial plankton during most of the year. This relates to severe climatic and seasonal conditions, and the lack of allochthonous carbon inputs to the system. Thus, high latitude

  20. Ellis-van Creveld syndrome and Weyers acrodental dysostosis are caused by cilia-mediated diminished response to hedgehog ligands.

    PubMed

    Ruiz-Perez, Victor L; Goodship, Judith A

    2009-11-15

    Ellis-van Creveld syndrome (EvC; OMIM 225500) is a recessive disorder comprising chondrodysplasia, polydactyly, nail dysplasia, orofacial abnormalities and, in a proportion of patients, cardiovascular malformations. Weyers acrodental dysostosis (Weyers; OMIM 193530) is an allelic dominant disorder comprising polydactyly, nail dysplasia, and orofacial abnormalities. EvC results from loss-of-function mutations in EVC or EVC2, the phenotype associated with the mutations in these two genes being indistinguishable. Three convincing causative mutations have been identified in patients with Weyers acrodental dysostosis, which are clustered in the last coding exon of EVC2 and lead to production of a truncated protein lacking the final 43 amino acids. Localization and function of EVC and EVC2 are inferred from studying the murine orthologs. Both Evc and Evc2 proteins localize to the basal bodies of primary cilia and analysis of an Ellis-van Creveld mouse model, which includes the limb shortening and tooth abnormalities of EvC patients, has demonstrated Hedgehog signaling defects in the absence of Evc. The loss of Evc2 has not been studied directly, but Hedgehog signaling is impaired when a mutant murine Evc2 Weyer variant is expressed in vitro. We conclude that the phenotypic abnormalities in EvC and Weyers syndrome result from tissue specific disruption of the response to Hh ligands.

  1. Biomimetic robotic Venus flytrap (Dionaea muscipula Ellis) made with ionic polymer metal composites.

    PubMed

    Shahinpoor, Mohsen

    2011-12-01

    The work described in this paper is a novel design of a robotic Venus flytrap (VFT) (Dionaea muscipula Ellis) by means of ionic polymeric metal composite (IPMC) artificial muscles as distributed nanosensors and nanoactuators. Rapid muscular movements in carnivorous plants, such as VFT, which are triggered by antenna-like sensors (trigger hair), present a golden key to study distributed biomolecular motors. Carnivorous plants, such as VFT, possess built-in intelligence (trigger hairs), as a strategy to capture prey, that can be turned on in a controlled manner. In the case of the VFT, the prey that is lured by the sweet nectar in the VFT pair of jaw-like lobes has to flip and move the trigger hairs, which are colorless, bristle-like and pointed. The dynamically moved trigger hairs then electro-elastically send an electric signal to the internal ions in the lobe to migrate outwardly for the jaw-like lobes to close rapidly to capture the prey. The manner in which the VFT lobes bend inward to capture the prey shows a remarkable similarity with typical IPMCs bending in an electric field. Furthermore, the mechano-electrical sensing characteristics of IPMCs also show a remarkable resemblance to mechano-electrical trigger hairs on the lobes of the VFT. The reader is referred to a number of papers in connection with sensing and actuation of IPMCs in particular. Thus, one can integrate IPMC lobes with a common electrode in the middle of one end of the lobes to act like a spine and use IPMC bristles as trigger finger to sense the intrusion of a fly or insect to send a sensing signal to a solid state relay which then triggers the actuation circuit of the IPMC lobes to rapidly bend toward each other and close. The two lobes, which form the trap, are attached to the midrib common electrode which is conveniently termed the spine. The upper surface of each lobe is dished, and spaced along the free margins of the lobes with some 15-20 prong-like teeth. These are tough and pointed

  2. Fructus Gardenia Extract ameliorates oxonate-induced hyperuricemia with renal dysfunction in mice by regulating organic ion transporters and mOIT3.

    PubMed

    Hu, Qing-Hua; Zhu, Ji-Xiao; Ji, Jing; Wei, Lin-Lin; Miao, Ming-Xing; Ji, Hui

    2013-07-29

    The potent anti-hyperuricemia activities of Fructus Gardenia Extract (FGE) have been well reported. The aim of this study was to evaluate the uricosuric and nephro-protective effects of FGE and explore its possible mechanisms of action in oxonate-induced hyperuricemic mice. FGE was orally administered to hyperuricemic and normal mice for 1 week. Serum and urinary levels of uric acid, creatinine and blood urea nitrogen (BUN), and fractional excretion of uric acid (FEUA) were measured. The mRNA and protein levels of mouse urate transporter 1 (mURAT1), glucose transporter 9 (mGLUT9), ATP-binding cassette, subfamily G, 2 (mABCG2), organic anion transporter 1 (mOAT1), mOAT3, oncoprotein induced transcript 3 (mOIT3), organic cation/carnitine transporters in the kidney were analyzed. Simultaneously, Tamm-Horsfall glycoprotein (THP) levels in urine and kidney were detected. FGE significantly reduced serum urate levels and increased urinary urate levels and FEUA in hyperuricemic mice. It could also effectively reverse oxonate-induced alterations in renal mURAT1, mGLUT9, mOAT1 and mOIT3 expressions, as well as THP levels, resulting in the enhancement of renal uric acid excretion. Moreover, FGE decreased serum creatinine and BUN levels, and up-regulated expression of organic cation/carnitine transporters, improving renal dysfunction in this model. Furthermore, FGE decreased renal mABCG2 expressions in hyperuricemic mice, contributing to its beneficial actions. However, further investigation is needed in clinical trials of FGE and its bioactive components.

  3. On the mechanism of trap closure of Venus flytrap (Dionaea muscipula Ellis).

    PubMed

    Hodick, D; Sievers, A

    1989-08-01

    The rapid trap closure of Dionaea muscinula Ellis has been explained by either a loss of turgor pressure of the upper epidermis, which should thus become flexible, or by a sudden acid-induced wall loosening of the motor cells. According to our experiments both explanations are doubtful. Objections against the turgor mechanism come from the determination by extracellular measurements from the upper epidermis of action-potential amplitudes before and after trap closure. Neither time course nor amplitude of the action potentials are altered by trap closure. In contrast a rise in the apoplastic concentration of K(+) or Na(+), which are the only ions present in the trap in osmotically significant concentrations, from 1 to 10 mM reduces the action-potential amplitudes by 25% and 15%, respectively. Furthermore, after trap closure the upper epidermal cells retain a considerable cell sap osmolality of 0.41 mol·kg(-1) which equals that of the mesophyll cells as determined by incipient plasmolysis. A sudden cell-wall acidification causing movement is improbable since an acidification of the apoplast from pH 6 to pH 4 reduces action-potential amplitudes by 33% whereas the amplitudes measured extracellylarly from the mesophyll and lower epidermis remain unchanged by trap closure. In addition, buffering the apoplast at pH 6 does not prevent movement in traps which have been incised several times from the margin to the midrib to facilitate buffer diffusion into the mesophyll. Even an alkalinization of cell walls of plasmolysed leaf segments to pH 9 does not prevent considerable extensions of the mesophyll and subsequent movement of the specimens during deplasmolysis.These experiments make it very likely that the mesophyll cells are already extensible but are kept compressed in the open trap, thus developing tissue tension. The mechanism which prevents their extension as long as the trap is open can so far only be explained for traps which have been paralysed by a long

  4. Identification of one novel mutation in the EVC2 gene in a Chinese family with Ellis-van Creveld syndrome.

    PubMed

    Zhang, Zeng; Bao, Kun; He, Jin-Wei; Fu, Wen-Zhen; Zhang, Chang-Qing; Zhang, Zhen-Lin

    2012-12-15

    Ellis-van Creveld syndrome (EvC) is a rare autosomal recessive skeletal dysplasia characterized by short limbs, short ribs, postaxial polydactyly, and dysplastic nails and teeth. It is caused by biallelic mutations in the EVC or EVC2 gene. Here, we identified a novel nonsense mutation p.W828X (c.2484G>A) in exon 14 and a recurrent nonsense mutation p. R399X (c.1195C>T) in exon 10 of EVC2 gene in a Chinese boy with EvC. Identification of a novel genotype in EvC will provide clues to the phenotype-genotype relations and may assist not only in the clinical diagnosis of EvC but also in the interpretation of genetic information used for prenatal diagnosis and genetic counseling.

  5. Geniposide protects against acute alcohol-induced liver injury in mice via up-regulating the expression of the main antioxidant enzymes.

    PubMed

    Wang, Junming; Zhang, Yueyue; Liu, Ruixin; Li, Xiaobing; Cui, Ying; Qu, Lingbo

    2015-04-01

    Geniposide (GP) is one of main compounds in Gardenia jasminoides Ellis, with both medicinal and nutritional value. This study was designed to determine, for the first time, how GP from G. jasminoides protects against acute alcohol-induced liver injury, and the underlying mechanisms. Mice were orally administered alcohol (6.0 g/kg body mass) 2 h after intragastric administration of GP and bifendate, every day for 7 continuous days. Six hours after the alcohol was administered, levels of serum alanine/aspartate transaminase (ALT/AST), hepatic lipid peroxidation (LPO), glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxidase (GPx), copper- and zinc-containing superoxide dismutase (CuZn-SOD), and catalase (CAT), and mRNA expression of CuZn-SOD and CAT were assayed. The results demonstrated that GP (20.0, 40.0, or 80 mg/kg) significantly reversed the excessive, alcohol-induced elevation in both serum ALT/AST and hepatic LPO levels. Moreover, hepatic GSH, GST, GPx, CuZn-SOD, and CAT levels were all decreased in the alcohol-treated mice, whereas treatment with GP reversed these decreases. Further analysis indicated that hepatic mRNA expression of CuZn-SOD and CAT in the alcohol-treated mice was significantly down-regulated, whereas GP up-regulated such decreases. Taken together, this study shows that GP protects against acute alcohol-induced liver injury via up-regulating the expression of the main antioxidant enzymes, and thus ameliorates alcohol-induced oxidative stress injury in the liver. PMID:25730420

  6. Geniposide protects against acute alcohol-induced liver injury in mice via up-regulating the expression of the main antioxidant enzymes.

    PubMed

    Wang, Junming; Zhang, Yueyue; Liu, Ruixin; Li, Xiaobing; Cui, Ying; Qu, Lingbo

    2015-04-01

    Geniposide (GP) is one of main compounds in Gardenia jasminoides Ellis, with both medicinal and nutritional value. This study was designed to determine, for the first time, how GP from G. jasminoides protects against acute alcohol-induced liver injury, and the underlying mechanisms. Mice were orally administered alcohol (6.0 g/kg body mass) 2 h after intragastric administration of GP and bifendate, every day for 7 continuous days. Six hours after the alcohol was administered, levels of serum alanine/aspartate transaminase (ALT/AST), hepatic lipid peroxidation (LPO), glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxidase (GPx), copper- and zinc-containing superoxide dismutase (CuZn-SOD), and catalase (CAT), and mRNA expression of CuZn-SOD and CAT were assayed. The results demonstrated that GP (20.0, 40.0, or 80 mg/kg) significantly reversed the excessive, alcohol-induced elevation in both serum ALT/AST and hepatic LPO levels. Moreover, hepatic GSH, GST, GPx, CuZn-SOD, and CAT levels were all decreased in the alcohol-treated mice, whereas treatment with GP reversed these decreases. Further analysis indicated that hepatic mRNA expression of CuZn-SOD and CAT in the alcohol-treated mice was significantly down-regulated, whereas GP up-regulated such decreases. Taken together, this study shows that GP protects against acute alcohol-induced liver injury via up-regulating the expression of the main antioxidant enzymes, and thus ameliorates alcohol-induced oxidative stress injury in the liver.

  7. Crocetin prevents retinal degeneration induced by oxidative and endoplasmic reticulum stresses via inhibition of caspase activity.

    PubMed

    Yamauchi, Mika; Tsuruma, Kazuhiro; Imai, Shunsuke; Nakanishi, Tomohiro; Umigai, Naofumi; Shimazawa, Masamitsu; Hara, Hideaki

    2011-01-10

    Crocetin is a carotenoid that is the aglicone of crocin, which are found in saffron stigmas (Crocus sativus L.) and gardenia fruit (Gardenia jasminoides Ellis). In this study, we investigated the effects of crocetin on retinal damage. To examine whether crocetin affects stress pathways, we investigated intracellular oxidation induced by reactive oxygen species, expression of endoplasmic reticulum (ER) stress-related proteins, disruption of the mitochondrial membrane potential (ΔΨ(m)), and caspases activation. In vitro, we employed cultured retinal ganglion cells (RGC-5, a mouse ganglion cell-line transformed using E1A virus). Cell damage was induced by tunicamycin or hydrogen peroxide (H(2)O(2)) exposure. Crocetin at a concentration of 3μM showed the inhibitory effect of 50-60% against tunicamycin- and H(2)O(2)-induced cell death and inhibited increase in caspase-3 and -9 activity. Moreover, crocetin inhibited the enzymatic activity of caspase-9 in a cell-free system. In vivo, retinal damage in mice was induced by exposure to white light at 8000lx for 3h after dark adaptation. Photoreceptor damage was evaluated by measuring the outer nuclear layer thickness at 5days after light exposure and recording the electroretinogram (ERG). Retinal cell damage was also detected with Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining at 48h after light exposure. Crocetin at 100mg/kg, p.o. significantly inhibited photoreceptor degeneration and retinal dysfunction and halved the expression of TUNEL-positive cells. These results indicate that crocetin has protective effects against retinal damage in vitro and in vivo, suggesting that the mechanism may inhibit increase in caspase-3 and -9 activities after retinal damage. PMID:20951131

  8. Crocetin, a carotenoid derivative, inhibits retinal ischemic damage in mice.

    PubMed

    Ishizuka, Fumiya; Shimazawa, Masamitsu; Umigai, Naofumi; Ogishima, Hiromi; Nakamura, Shinsuke; Tsuruma, Kazuhiro; Hara, Hideaki

    2013-03-01

    Crocetin, an aglycone of crocin, is found both in the saffron crocus (Crocus starus L.) and in gardenia fruit (Gardenia jasminoides Ellis). We evaluated the protective effects of crocetin against the retinal ischemia induced by 5h unilateral ligation of both the pterygopalatine artery (PPA) and the external carotid artery (ECA) in anesthetized mice. The effects of crocetin (20mg/kg, p.o.) on ischemia/reperfusion-induced retinal damage were examined by histological, electrophysiological, and anti-apoptotic analyses. Data for anti-apoptotic analysis was obtained by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Using immunohistochemistry and immunoblotting, the protective mechanism mediating the effects of crocetin was evaluated by examining crocetin's effects on the expression of 8-hydroxy-2-deoxyguanosine (8-OHdG; used as a marker of oxidative stress) and on phosphorylations of mitogen-activated protein kinases [MAPK; viz. extracellular signal-regulated protein kinases (ERK), c-Jun N-terminal kinases (JNK) and p38], and the redox-sensitive transcription factors nuclear factor-kappa B (NF-κB) and c-Jun. The histological analysis revealed that ischemia/reperfusion (I/R) decreased the cell number in the ganglion cell layer (GCL) and the thickness of inner nuclear layer (INL), and that crocetin inhibited GCL and INL. ERG measurements revealed that crocetin prevented the I/R-induced reductions in a- and b-wave amplitudes seen at 5 days after I/R. In addition, crocetin decreased the numbers of TUNEL-positive cells and 8-OHdG-positive cells, and the phosphorylation levels of p38, JNK, NF-κB, and c-Jun present in the retina after I/R. These findings indicate that crocetin prevented ischemia-induced retinal damage through its inhibition of oxidative stress. PMID:23428630

  9. Comparative pharmacokinetics and brain distribution of magnolol and honokiol after oral administration of Magnolia officinalis cortex extract and its compatibility with other herbal medicines in Zhi-Zi-Hou-Po Decoction to rats.

    PubMed

    Liu, Yuan; Wang, Dan; Yang, Gongjun; Shi, Qingshui; Feng, Fang

    2016-03-01

    Zhi-Zi-Hou-Po decoction (ZZHPD) is one of the famous antidepressant Chinese formulas and is composed of Magnolia officinalis cortex (HP), Gardenia jasminoides Ellis (ZZ) and Citrus aurantium L. (ZS). Magnolol (MN) and honokiol (HN) from HP are the major active ingredients responsible for the therapeutic effects of ZZHPD. The aim of this study is to compare the pharmacokinetics and rat brain distribution of MN and HN after oral administration of HP extract and its compatibility with other herbal medicines in ZZHPD by HPLC-FLD. Compared with the HP group, Tmax (time to reach peak drug concentration in plasma) and AUC(0-τ) significantly increased in the ZZHPD and HP-ZZ groups. There was little change in the HP-ZS group in comparison with the HP group, which indicated that ZZ promotes absorption extent and defers the absorption rate of MN. The different compatibility of ZZHPD had a different degree of impact on the concentration of MN and HN in brain. The concentration of MN significantly increased in the HP-ZZ group while it decreased in the HP-ZS group compared with the HP group, which explained the concentration of compounds being slightly greater in the ZZHPD group than in the HP group. HP mixed with other medicines resulted in a decrease in HN concentration in the brain, particularly HP compatible with ZS. The results could be helpful for revealing the compatibility mechanism and providing clinical medication guidance for ZZHPD.

  10. Pharmacokinetics of geniposide by monoclonal antibody-based icELISA in mice after oral administration of Huanglian-Jiedu-Tang.

    PubMed

    Qu, Hui-Hua; Sun, Ye; Wu, Ting-Ting; Zhang, Gui-Liang; Cheng, Jin-Jun; Wang, Xue-Qian; Feng, Hui-Bin; Zhao, Yan; Wang, Qing-Guo

    2014-01-01

    Geniposide, Geniposide, the main active component in extracts of Gardenia jasminoides ELLIS., is one of the main components of Huanglian-Jiedu-Tang (HJT). This study aimed to validate an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on monoclonal antibodies (mAb) against geniposide (anti-geniposide mAb), which was developed by our lab, and apply the assay to study the pharmacokinetics of geniposide in HJT in mice. Blood samples were drawn from mice at predetermined time points after oral administration of HJT in three dosages. A linear correlation was obtained for geniposide concentrations in the range from 1.17 to 37.50 µg/mL. The intra-day and inter-day precision values of the icELISA method were well within the recommended range (≤10%). The recovery rates ranged from 99.74 to 102.40%. Stability studies showed that geniposide sample solutions were intact for 12 h. The Tmax and mean residence time (MRT) of geniposide of the three groups were consistent with previous data. The results suggest that a reliable and effective method was established and could be applied to the study of the pharmacokinetics of geniposide in HJT. PMID:25177035

  11. Proteomic investigation of signatures for geniposide-induced hepatotoxicity.

    PubMed

    Wei, Junying; Zhang, Fangbo; Zhang, Yi; Cao, Chunyu; Li, Xianyu; Li, Defeng; Liu, Xin; Yang, Hongjun; Huang, Luqi

    2014-12-01

    Evaluating the safety of traditional medicinal herbs and their major active constituents is critical for their widespread usage. Geniposide, a major active constituent with a defined structure from the traditional medicinal herb Gardenia jasminoides ELLIS fruit, exhibits remarkable anti-inflammatory, antiapoptotic, and antifibrotic properties and has been used in a variety of medical fields, mainly for the treatment of liver diseases. However, geniposide-induced hepatotoxicity and methods for the early detection of hepatotoxicity have yet to be reported. In this study, geniposide-induced hepatotoxicity was investigated. In addition, candidate biomarkers for the earlier detection of geniposide-induced hepatotoxicity were identified using a label-free quantitative proteomics approach on a geniposide overdose-induced liver injury in a rat model. Using an accurate intensity-based, absolute quantification (iBAQ)-based, one-step discovery and verification approach, a candidate biomarker panel was easily obtained from individual samples in response to different conditions. To determine the biomarkers' early detection abilities, five candidate biomarkers were selected and tested using enzyme-linked immunosorbent assays (ELISAs). Two biomarkers, glycine N-methyltransferase (GNMT) and glycogen phosphorylase (PYGL), were found to indicate hepatic injuries significantly earlier than the current gold standard liver biomarker. This study provides a first insight into geniposide-induced hepatotoxicity in a rat model and describes a method for the earlier detection of this hepatotoxicity, facilitating the efficient monitoring of drug-induced hepatotoxicity. PMID:25336395

  12. Inhibitory effect of plant-originated glycoprotein (27 kDa) on expression of matrix metalloproteinase-9 in cadmium chloride-induced BNL CL.2 cells.

    PubMed

    Lee, Jin; Lim, Kye-Taek

    2011-12-01

    Cadmium is very harmful to the environment and to human beings because of its long lifetime. The toxicity of cadmium as an industrial pollutant and a food contaminant, and as one of the major components in cigarette smoke is well known. Cadmium can cause a number of lesions in many organs, such as the kidney, the lung, the liver, the brain, the blood system. However, the mechanism of toxicity of cadmium is not yet clear. Also, it has been well known as human carcinogen which is indirectly caused inflammation-mediated hepatocarcinoma. In the present study it was demonstrated that glycoprotein (27 kDa) isolated from Gardenia jasminoides Ellis (GJE) protects BNL CL.2 cells from expression of inflammation-related factors stimulated by cadmium chloride (10 μM). Intracellular ROS and intracellular Ca(2+) using fluorescence, activities of activator protein (AP)-1, cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9, and arachidonic acid (AA) using immunoblot analysis or radioactivity were evaluated. The results obtained from this experiment indicated that GJE glycoprotein (100 μg/mL) inhibits the production of intracellular ROS, and intracellular Ca(2+) mobilization. Also, it significantly suppressed inflammatory factors [expression of AP-1 (c-Jun and c-Fos), arachidonic acid, COX-2, and MMP-9]. Taken together, these findings suggest that GJE glycoprotein might be used for protection of inflammation caused by cadmium ion as one of natural compounds. PMID:21924884

  13. Pharmacokinetics of geniposide in Zhi-Zi-Hou-Pu decoction and in different combinations of its constituent herbs.

    PubMed

    Sun, Yanqing; Feng, Fang; Yu, Xiupei

    2012-01-01

    Zhi-Zi-Hou-Pu decoction (ZZHPD), a classic antidepressant formula, is composed of Gardenia jasminoides Ellis (ZZ), Fructus aurantii immaturus (ZS) and Cortex magnoliae officinalis (HP). ZZHPD has attracted a great deal of attention for its antidepressant effects. The aim of this study was to compare the pharmacokinetics of geniposide (one of the predominant active ingredients) after oral administration of different combinations of ZZHPD and to explore the influence of herb-herb interaction on the pharmacokinetics of geniposide. Twenty four rats were divided randomly into four groups and were administered one of the four extracts: ZZ, ZZ-HP, ZZ-ZS and ZZHPD (ZZ-HP-ZS) via intragastric gavage with approximately the same dose of 40.65 mg/kg geniposide (an effective human daily dose of ZZHPD). Plasma concentrations of geniposide were determined using an HPLC method. Pharmacokinetic parameters were calculated from the plasma concentration-time data. Compared with ZZ alone, the ZZ-ZS combination delayed T(max) and ZZ-HP, ZZ-ZS, ZZHPD remarkably shortened the T(1/2) of geniposide. In addition, ZZ-HP, ZZ-ZS, ZZHPD obviously increased the AUC of geniposide. The result illustrated that the oral bioavailability of geniposide was dramatically enhanced when ZZ was combined with HP or/and ZS. It can be deduced that herb-herb interaction may increase the absorption, and significantly improve the oral bioavailability of geniposide in rats. PMID:21544883

  14. Protective effect of crocetin on hemorrhagic shock-induced acute renal failure in rats.

    PubMed

    Wang, Yunbo; Yan, Junling; Xi, Liang; Qian, Zhiyu; Wang, Zhenghong; Yang, Lina

    2012-07-01

    Multiple organ failure is a common outcome of hemorrhagic shock followed by resuscitation, and the kidney is one of the prime target organs involved. The main objective of the study was to evaluate whether crocetin, a natural product from Gardenia jasminoides Ellis, has beneficial effects on renal dysfunction caused by hemorrhagic shock and resuscitation in rats. Anesthetized rats were bled to reduce mean arterial blood pressure to 35 (SD, 5) mmHg for 60 min and then were resuscitated with their withdrawn shed blood and normal saline. Crocetin was administered via the duodenum at a dose of 50 mg/kg 40 min after hemorrhage. The increase in creatinine and blood urea nitrogen was significantly reduced at 2 h after hemorrhage and resuscitation in crocetin-treated rats. The increases in renal nitric oxide, tumor necrosis factor α, and interleukin 6 were also attenuated by crocetin. Hemorrhagic shock resulted in a significant elevation in malondialdehyde production and was accompanied by a reduction in total superoxide dismutase activity, activation of nuclear factor κB, and overexpression of inducible nitric oxide synthase. These changes were significantly attenuated by crocetin at 2 h after resuscitation. These results suggested that crocetin blocks inflammatory cascades by inhibiting production of reactive oxygen species and restoring superoxide dismutase activity to ameliorate renal dysfunction caused by hemorrhage shock and resuscitation. PMID:22576007

  15. Metabolomics and proteomics annotate therapeutic properties of geniposide: targeting and regulating multiple perturbed pathways.

    PubMed

    Wang, Xijun; Zhang, Aihua; Yan, Guangli; Sun, Wenjun; Han, Ying; Sun, Hui

    2013-01-01

    Geniposide is an important constituent of Gardenia jasminoides Ellis, a famous Chinese medicinal plant, and has displayed bright prospects in prevention and therapy of hepatic injury (HI). Unfortunately, the working mechanisms of this compound are difficult to determine and thus remain unknown. To determine the mechanisms that underlie this compound, we conducted a systematic analysis of the therapeutic effects of geniposide using biochemistry, metabolomics and proteomics. Geniposide significantly intensified the therapeutic efficacy as indicated by our modern biochemical analysis. Metabolomics results indicate 9 ions in the positive mode as differentiating metabolites which were associated with perturbations in primary bile acid biosynthesis, butanoate metabolism, citrate cycle (TCA cycle), alanine, aspartate and glutamate metabolism. Of note, geniposide has potential pharmacological effect through regulating multiple perturbed pathways to normal state. In an attempt to address the benefits of geniposide based on the proteomics approaches, the protein-interacting networks were constructed to aid identifying the drug targets of geniposide. Six identified differential proteins appear to be involved in antioxidation and signal transduction, energy production, immunity, metabolism, chaperoning. These proteins were closely related in the protein-protein interaction network and the modulation of multiple vital physiological pathways. These data will help to understand the molecular therapeutic mechanisms of geniposide on hepatic damage rats. We also conclude that metabolomics and proteomics are powerful and versatile tools for both biomarker discovery and exploring the complex relationships between biological pathways and drug response, highlighting insights into drug discovery. PMID:23967205

  16. Identification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.

    PubMed

    Han, Han; Yang, Li; Xu, Ying; Ding, Yue; Bligh, S W Annie; Zhang, Tong; Wang, Zhengtao

    2011-11-15

    Geniposide, an iridoid glycoside, is an important and characteristic compound in the fruits of Gardenia jasminoides Ellis, a commonly used medicinal herb in Chinese traditional and folk medicine for the treatment of inflammation and jaundice. However, few studies have been carried out on the metabolism of geniposide. In this study, we have established a rapid and sensitive method using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC/ESI-QTOF-MS) for analysis of the metabolic profile of geniposide in rat urine after oral administration. A total of ten metabolites were detected and identified by comparing their fragmentation patterns with that of geniposide using Metabolynx™ and MassFragment™ software tools. The results revealed that the principal metabolism pathways of geniposide in rat occurred after deglycosylation of the irdoid glycoside take place and this is followed by glucuronidation and the pyran-ring cleavages. The major metabolite, the glucuronic acid conjugate of genipin as observed in vivo, was further confirmed by the in vitro enzymatic study. The results of this work have demonstrated the feasibility of the UPLC/ESI-QTOF-MS approach for rapid and reliable characterization of metabolites from iridoid compounds. PMID:22006398

  17. Neuroprotective effects of geniposide in the MPTP mouse model of Parkinson's disease.

    PubMed

    Chen, YiMei; Zhang, Yanfang; Li, Lin; Hölscher, Christian

    2015-12-01

    Parkinson's disease (PD) is a chronic neurodegenerative disease, and there is no cure for it at present. We tested the drug Geniposide, an active component of Gardenia jasminoides Ellis which is used in traditional Chinese medicine. Geniposide has shown neuroprotective and growth-factor like effects in several in vivo and in vitro studies. In the present study, Geniposide had been tested in an acute PD mouse model induced by four 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) intraperitoneal injections. Geniposide treatment (100mg/kg ip.) for 8 days after MPTP treatment (30mg/kg ip.) improved the locomotor and exploratory activity of mice (open field), and improved bradykinesia and movement balance of mice (rotarod, swim test). Geniposide treatment also restored tyrosine hydroxylase (TH) positive dopaminergic neuron numbers in the substantia nigra pars compacta. Drug treatment also increased levels of growth factor signaling molecule Bax and reduced the apoptosis signaling molecule Bcl-2. Caspase 3 activation was also reduced in the substantia nigra. We conclude that Geniposide exerted its neuroprotective effect by enhancing growth factor signaling and the reduction of apoptosis. Geniposide is an ingredient in Chinese traditional medicine with few known side effects and shows potential as a drug treatment for Parkinson's disease. PMID:26409043

  18. Anti-inflammatory effects and pharmacokinetics study of geniposide on rats with adjuvant arthritis.

    PubMed

    Chen, Jin-Yun; Wu, Hong; Li, Hui; Hu, Shun-Li; Dai, Miao-miao; Chen, Jian

    2015-01-01

    The aim of this study was to explore the anti-inflammatory effects of Geniposide (GE), an iridoid glycoside compound extracted from Gardenia jasminoides Ellis (GJ) fruit in adjuvant-induced arthritis (AA) rats and its pharmacokinetic (PK) basis. AA was induced by injecting with Freund's complete adjuvant (FCA). Male SD rats were subjected to treatment with GE (30, 60 and 120mg/kg) from day 17 to 24 after immunization. Fibroblast-like synoviocyte (FLS) proliferation was assessed by MTT. Interleukin (IL)-1, IL-6, TNF-α and IL-10 were determined using double-sandwich enzyme-linked immunosorbent assay (ELISA). Expression of p38 mitogen-activated protein kinases (p38MAPKs) related proteins in FLS was detected by Western blotting. PK profiles were simultaneously detected by ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) in AA rat plasma after oral administration of GE on day 17 after immunization. As a result, GE promoted the recovery of arthritis and inhibited the colonic inflammation damage in AA rats by decreasing the expression level of TNF-α, IL-1 and IL-6, increasing the production of IL-10 and inhibiting the expression of phospho-p38 (p-p38) related proteins in FLS. PK parameters (AUC, Cmax and t1/2) tended to be associated with dosage-related decreasing of efficacy index. PMID:25434608

  19. Identification and distribution of four metabolites of geniposide in rats with adjuvant arthritis.

    PubMed

    Chen, Jian; Wu, Hong; Dai, Miao-Miao; Li, Hui; Chen, Jin-Yun; Hu, Shun-Li

    2014-09-01

    Geniposide (GE), also called Jasminoidin, is the major active ingredient of Gardenia jasminoides Ellis (GJ) fruit, which has long been used in traditional Chinese medicine (TCM). Growing evidences suggested that GE has a great potentiality for treating rheumatoid arthritis (RA). However, GE is rapidly metabolized, and we know little about its availability or metabolites in tissues. To elucidate the distribution of GE and its metabolites in tissues, three groups of adjuvant arthritis (AA) rats were given GE (33, 66 and 120 mg/kg) from days 18 to 24, and the biotransformation of GE in plasma, liver, spleen, synovium, urine and mesenteric lymph node (MLN) of rats was investigated by a novel approach named Information-Dependent Acquisition (IDA)-Mediated LC-MS/MS method. As a result, GE and its four major metabolites were detected as follows: GE, G1, G2 in plasma; GE, G2 in MLNs; only GE in liver and synovium; GE, G2, G3 and G4 in spleen; and GE, G1, G2 and G4 in urine. In total four metabolites (G1-G4) involved in the in vivo metabolism processes were identified. The results of this work have demonstrated the IDA-Mediated LC-MS/MS could screen rapidly and reliably the characterization of metabolites from iridoid compounds. PMID:24910002

  20. Evaluation of genipin on human cytochrome P450 isoenzymes and P-glycoprotein in vitro.

    PubMed

    Gao, Li-Na; Zhang, Ye; Cui, Yuan-Lu; Yan, Kuo

    2014-10-01

    Genipin is obtained from the fruit of Gardenia jasminoides Ellis and acts as an herbal medicine or functional food in East Asia. In addition to produce natural colorant, it possesses widely antiinflammatory, antithrombotic, antidepressive and anticarcinogenic activities. However, little research focuses on the potential of genipin for drug-drug interactions. In this study, effects of genipin on mRNA and protein expression of cytochrome P450 (CYP) 2C19, CYP2D6 and CYP3A4 were detected by real-time reverse-transcription polymerase chain reaction (real-time RT-PCR) and Western blot, respectively, in human hepatoma HepG2 cells. Enzyme activities of which were detected by luminogenic CYP assay in vitro. Moreover, effect of genipin on P-glycoprotein expression was analyzed by Western blot. Results showed that genipin possessed a significant induction on CYP2D6 and a remarkable inhibition on CYP2C19 and CYP3A4 not only from the expression of mRNA and protein (P<0.05 or P<0.01), but the level of enzyme activity. Moreover, a concentration-dependent induction of genipin on P-glycoprotein expression was observed. In conclusion, caution should be exercised with respect to the induction or inhibition of genipin on CYP isoenzymes and the strong induction on P-glycoprotein. PMID:25073096

  1. Genipin stimulates glucose transport in C2C12 myotubes via an IRS-1 and calcium-dependent mechanism.

    PubMed

    Ma, Chan-Juan; Nie, Ai-Fang; Zhang, Zhi-Jian; Zhang, Zhi-Guo; Du, Li; Li, Xiao-Ying; Ning, Guang

    2013-03-01

    Genipin, a compound derived from Gardenia jasminoides Ellis fruits, has been used over the years in traditional Chinese medicine to treat symptoms of type 2 diabetes. However, the molecular basis for its antidiabetic effect has not been fully revealed. In this study, we investigated the effects of genipin on glucose uptake and signaling pathways in C(2)C(12) myotubes. Our study demonstrates that genipin stimulated glucose uptake in a time- and dose-dependent manner. The maximal effect was achieved at 2 h with a concentration of 10 μM. In myotubes, genipin promoted glucose transporter 4 (GLUT4) translocation to the cell surface, which was observed by analyzing their distribution in subcellular membrane fraction, and increased the phosphorylation of insulin receptor substrate-1 (IRS-1), AKT, and GSK3β. Meanwhile, genipin increased ATP levels, closed K(ATP) channels, and then increased the concentration of calcium in the cytoplasm in C(2)C(12) myotubes. Genipin-stimulated glucose uptake could be blocked by both the PI3-K inhibitor wortmannin and calcium chelator EGTA. Moreover, genipin increases the level of reactive oxygen species and ATP in C(2)C(12) myotubes. These results suggest that genipin activates IRS-1, PI3-K, and downstream signaling pathway and increases concentrations of calcium, resulting in GLUT4 translocation and glucose uptake increase in C(2)C(12) myotubes. PMID:23257267

  2. (1)H NMR-based metabolic profiling of liver in chronic unpredictable mild stress rats with genipin treatment.

    PubMed

    Chen, Jian-Li; Shi, Bi-Yun; Xiang, Huan; Hou, Wen-Jing; Qin, Xue-Mei; Tian, Jun-Sheng; Du, Guan-Hua

    2015-11-10

    Genipin, a hydrolyzed metabolite of geniposide extracted from the fruit of Gardenia jasminoides Ellis, has shown promise in alleviating depressive symptoms, however, the antidepressant mechanism of genipin remains unclear and incomprehensive. In this study, the metabolic profiles of aqueous and lipophilic extracts in liver of the chronic unpredictable mild stress (CUMS)-induced rat with genipin treatment were investigated using proton nuclear magnetic resonance ((1)H NMR) spectroscopy coupled with multivariate data analysis. Significant differences in the metabolic profiles of rats in the CUMS model group (MS) and the control group (NS) were observed with metabolic effects including decreasing in choline, glycerol and glycogen, increasing in lactate, alanine and succinate, and a disordered lipid metabolism, while the moderate dose (50mg/kg) of genipin could significantly regulate the concentrations of glycerol, lactate, alanine, succinate and the lipid to their normal levels. These biomakers were involved in metabolism pathways such as glycolysis/gluconeogensis, tricarboxylic acid (TCA) cycle and lipid metabolism, which may be helpful for understanding of antidepressant mechanism of genipin. PMID:26204246

  3. Metabolomics coupled with proteomics advancing drug discovery toward more agile development of targeted combination therapies.

    PubMed

    Wang, Xijun; Zhang, Aihua; Wang, Ping; Sun, Hui; Wu, Gelin; Sun, Wenjun; Lv, Haitao; Jiao, Guozheng; Xu, Hongying; Yuan, Ye; Liu, Lian; Zou, Dixin; Wu, Zeming; Han, Ying; Yan, Guangli; Dong, Wei; Wu, Fangfang; Dong, Tianwei; Yu, Yang; Zhang, Shuxiang; Wu, Xiuhong; Tong, Xin; Meng, Xiangcai

    2013-05-01

    To enhance the therapeutic efficacy and reduce the adverse effects of traditional Chinese medicine, practitioners often prescribe combinations of plant species and/or minerals, called formulae. Unfortunately, the working mechanisms of most of these compounds are difficult to determine and thus remain unknown. In an attempt to address the benefits of formulae based on current biomedical approaches, we analyzed the components of Yinchenhao Tang, a classical formula that has been shown to be clinically effective for treating hepatic injury syndrome. The three principal components of Yinchenhao Tang are Artemisia annua L., Gardenia jasminoids Ellis, and Rheum Palmatum L., whose major active ingredients are 6,7-dimethylesculetin (D), geniposide (G), and rhein (R), respectively. To determine the mechanisms underlying the efficacy of this formula, we conducted a systematic analysis of the therapeutic effects of the DGR compound using immunohistochemistry, biochemistry, metabolomics, and proteomics. Here, we report that the DGR combination exerts a more robust therapeutic effect than any one or two of the three individual compounds by hitting multiple targets in a rat model of hepatic injury. Thus, DGR synergistically causes intensified dynamic changes in metabolic biomarkers, regulates molecular networks through target proteins, has a synergistic/additive effect, and activates both intrinsic and extrinsic pathways.

  4. Crocetin protects ultraviolet A-induced oxidative stress and cell death in skin in vitro and in vivo.

    PubMed

    Ohba, Takuya; Ishisaka, Mitsue; Tsujii, Saori; Tsuruma, Kazuhiro; Shimazawa, Masamitsu; Kubo, Koya; Umigai, Naofumi; Iwawaki, Takao; Hara, Hideaki

    2016-10-15

    Crocetin, the aglycone of crocin, is a carotenoid found in fruits of gardenia (Gardeina jasminoides Ellis) and saffron (Crocus sativus L.). We investigated the protective effects of crocetin against ultraviolet-A (UV-A)-induced skin damage and explored the underlying mechanism. Human skin-derived fibroblasts cells (NB1-RGB) were damaged by exposure to UV-A irradiation (10J/cm(2)). Crocetin protected these cells against cell death and reduced the production of reactive oxygen species induced by UV-A irradiation. Crocetin treatment also suppressed induction of caspase-3 activation by UV-A irradiation. The effects of crocetin against oxidative stress were also examined by imaging of Keap1-dependent oxidative stress detector (OKD) mice. UV-A irradiation upregulated oxidative stress in the OKD mice skin, while crocetin administration (100mg/kg, p.o.) ameliorated this oxidative stress. Crocetin administration also decreased lipid peroxidation in the skin. These findings suggest that crocetin its observed protective effects against UV-A induced skin damage by reducing reactive oxygen species production and cell apoptosis. PMID:27452919

  5. Crocetin ester improves myocardial ischemia via Rho/ROCK/NF-κB pathway.

    PubMed

    Huang, Zhiheng; Nan, Chen; Wang, Hanqing; Su, Qiang; Xue, Wenda; Chen, Yanyan; Shan, Xin; Duan, Jinao; Chen, Gang; Tao, Weiwei

    2016-09-01

    Crocetin ester (CE) is the active ingredient of Crocus sativus L. stigmas and Gardenia jasminoides Ellis fruit. The main purpose of the present study was to investigate the protective effect of CE on isoproterenol (ISO)-induced acute myocardial ischemia (AMI) through Rho/ROCK/NF-κB pathway and explore its underlying mechanism. Administration of CE (25 and 50mg/kg) could significantly reduce the serum contents of pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). In addition, pretreatment with CE attenuated the contents of creatine kinase (CK), malondialdehyde (MDA) and the activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD) in serum. Treatment with CE also improved the histopathological alteration and decreased the ST elevation. Furthermore, CE could ameliorate the cardiac expressions of Cu, Zn-superoxide dismutase (SOD1), MDA5, Rho, ROCK, p-IκB and p-NF-κBp65 in ISO-induced rats. It was assumed that CE might be a new therapeutic candidate for the treatment of AMI possibly through the inhibition of Rho/ROCK/NF-κB pathway. PMID:27285672

  6. Proteomic investigation of signatures for geniposide-induced hepatotoxicity.

    PubMed

    Wei, Junying; Zhang, Fangbo; Zhang, Yi; Cao, Chunyu; Li, Xianyu; Li, Defeng; Liu, Xin; Yang, Hongjun; Huang, Luqi

    2014-12-01

    Evaluating the safety of traditional medicinal herbs and their major active constituents is critical for their widespread usage. Geniposide, a major active constituent with a defined structure from the traditional medicinal herb Gardenia jasminoides ELLIS fruit, exhibits remarkable anti-inflammatory, antiapoptotic, and antifibrotic properties and has been used in a variety of medical fields, mainly for the treatment of liver diseases. However, geniposide-induced hepatotoxicity and methods for the early detection of hepatotoxicity have yet to be reported. In this study, geniposide-induced hepatotoxicity was investigated. In addition, candidate biomarkers for the earlier detection of geniposide-induced hepatotoxicity were identified using a label-free quantitative proteomics approach on a geniposide overdose-induced liver injury in a rat model. Using an accurate intensity-based, absolute quantification (iBAQ)-based, one-step discovery and verification approach, a candidate biomarker panel was easily obtained from individual samples in response to different conditions. To determine the biomarkers' early detection abilities, five candidate biomarkers were selected and tested using enzyme-linked immunosorbent assays (ELISAs). Two biomarkers, glycine N-methyltransferase (GNMT) and glycogen phosphorylase (PYGL), were found to indicate hepatic injuries significantly earlier than the current gold standard liver biomarker. This study provides a first insight into geniposide-induced hepatotoxicity in a rat model and describes a method for the earlier detection of this hepatotoxicity, facilitating the efficient monitoring of drug-induced hepatotoxicity.

  7. Chemical profiling approach to evaluate the influence of traditional and simplified decoction methods on the holistic quality of Da-Huang-Xiao-Shi decoction using high-performance liquid chromatography coupled with diode-array detection and time-of-flight mass spectrometry.

    PubMed

    Yan, Xuemei; Zhang, Qianying; Feng, Fang

    2016-04-01

    Da-Huang-Xiao-Shi decoction, consisting of Rheum officinale Baill, Mirabilitum, Phellodendron amurense Rupr. and Gardenia jasminoides Ellis, is a traditional Chinese medicine used for the treatment of jaundice. As described in "Jin Kui Yao Lue", a traditional multistep decoction of Da-Huang-Xiao-Shi decoction was required while simplified one-step decoction was used in recent repsorts. To investigate the chemical difference between the decoctions obtained by the traditional and simplified preparations, a sensitive and reliable approach of high-performance liquid chromatography coupled with diode-array detection and electrospray ionization time-of-flight mass spectrometry was established. As a result, a total of 105 compounds were detected and identified. Analysis of the chromatogram profiles of the two decoctions showed that many compounds in the decoction of simplified preparation had changed obviously compared with those in traditional preparation. The changes of constituents would be bound to cause the differences in the therapeutic effects of the two decoctions. The present study demonstrated that certain preparation methods significantly affect the holistic quality of traditional Chinese medicines and the use of a suitable preparation method is crucial for these medicines to produce special clinical curative effect. This research results elucidated the scientific basis of traditional preparation methods in Chinese medicines.

  8. Genetic and Epigenetic Approaches for the Possible Detection of Adulteration and Auto-Adulteration in Saffron (Crocus sativus L.) Spice.

    PubMed

    Soffritti, Giovanna; Busconi, Matteo; Sánchez, Rosa Ana; Thiercelin, Jean-Marie; Polissiou, Moschos; Roldán, Marta; Fernández, José Antonio

    2016-01-01

    Saffron (Crocus sativus L.) is very expensive and, because of this, often subject to adulteration. Modern genetic fingerprinting techniques are an alternative low cost technology to the existing chemical techniques, which are used to control the purity of food products. Buddleja officinalis Maxim, Gardenia jasminoides Ellis, Curcuma longa L., Carthamus tinctorius L. and Calendula officinalis L. are among the most frequently-used adulterants in saffron spice. Three commercial kits were compared concerning the ability to recover PCR-grade DNA from saffron, truly adulterated samples and possible adulterants, with a clear difference among them, mainly with the processed samples. Only one of the three kits was able to obtain amplifiable DNA from almost all of the samples, with the exception of extracts. On the recovered DNA, new markers were developed based on the sequence of the plastid genes matK and rbcL. These primers, mainly those developed on matK, were able to recognize saffron and the adulterant species and also in mixtures with very low percentages of adulterant. Finally, considering that the addition of different parts of saffron flowers is one of the most widespread adulterations, by analyzing the DNA of the different parts of the flower (styles, stamens and tepals) at the genetic and epigenetic level, we succeeded in finding differences between the three tissues that can be further evaluated for a possible detection of the kind of fraud.

  9. Genetic and Epigenetic Approaches for the Possible Detection of Adulteration and Auto-Adulteration in Saffron (Crocus sativus L.) Spice.

    PubMed

    Soffritti, Giovanna; Busconi, Matteo; Sánchez, Rosa Ana; Thiercelin, Jean-Marie; Polissiou, Moschos; Roldán, Marta; Fernández, José Antonio

    2016-01-01

    Saffron (Crocus sativus L.) is very expensive and, because of this, often subject to adulteration. Modern genetic fingerprinting techniques are an alternative low cost technology to the existing chemical techniques, which are used to control the purity of food products. Buddleja officinalis Maxim, Gardenia jasminoides Ellis, Curcuma longa L., Carthamus tinctorius L. and Calendula officinalis L. are among the most frequently-used adulterants in saffron spice. Three commercial kits were compared concerning the ability to recover PCR-grade DNA from saffron, truly adulterated samples and possible adulterants, with a clear difference among them, mainly with the processed samples. Only one of the three kits was able to obtain amplifiable DNA from almost all of the samples, with the exception of extracts. On the recovered DNA, new markers were developed based on the sequence of the plastid genes matK and rbcL. These primers, mainly those developed on matK, were able to recognize saffron and the adulterant species and also in mixtures with very low percentages of adulterant. Finally, considering that the addition of different parts of saffron flowers is one of the most widespread adulterations, by analyzing the DNA of the different parts of the flower (styles, stamens and tepals) at the genetic and epigenetic level, we succeeded in finding differences between the three tissues that can be further evaluated for a possible detection of the kind of fraud. PMID:26978342

  10. Comparative pharmacokinetics and brain distribution of magnolol and honokiol after oral administration of Magnolia officinalis cortex extract and its compatibility with other herbal medicines in Zhi-Zi-Hou-Po Decoction to rats.

    PubMed

    Liu, Yuan; Wang, Dan; Yang, Gongjun; Shi, Qingshui; Feng, Fang

    2016-03-01

    Zhi-Zi-Hou-Po decoction (ZZHPD) is one of the famous antidepressant Chinese formulas and is composed of Magnolia officinalis cortex (HP), Gardenia jasminoides Ellis (ZZ) and Citrus aurantium L. (ZS). Magnolol (MN) and honokiol (HN) from HP are the major active ingredients responsible for the therapeutic effects of ZZHPD. The aim of this study is to compare the pharmacokinetics and rat brain distribution of MN and HN after oral administration of HP extract and its compatibility with other herbal medicines in ZZHPD by HPLC-FLD. Compared with the HP group, Tmax (time to reach peak drug concentration in plasma) and AUC(0-τ) significantly increased in the ZZHPD and HP-ZZ groups. There was little change in the HP-ZS group in comparison with the HP group, which indicated that ZZ promotes absorption extent and defers the absorption rate of MN. The different compatibility of ZZHPD had a different degree of impact on the concentration of MN and HN in brain. The concentration of MN significantly increased in the HP-ZZ group while it decreased in the HP-ZS group compared with the HP group, which explained the concentration of compounds being slightly greater in the ZZHPD group than in the HP group. HP mixed with other medicines resulted in a decrease in HN concentration in the brain, particularly HP compatible with ZS. The results could be helpful for revealing the compatibility mechanism and providing clinical medication guidance for ZZHPD. PMID:26173910

  11. Chemical profiling approach to evaluate the influence of traditional and simplified decoction methods on the holistic quality of Da-Huang-Xiao-Shi decoction using high-performance liquid chromatography coupled with diode-array detection and time-of-flight mass spectrometry.

    PubMed

    Yan, Xuemei; Zhang, Qianying; Feng, Fang

    2016-04-01

    Da-Huang-Xiao-Shi decoction, consisting of Rheum officinale Baill, Mirabilitum, Phellodendron amurense Rupr. and Gardenia jasminoides Ellis, is a traditional Chinese medicine used for the treatment of jaundice. As described in "Jin Kui Yao Lue", a traditional multistep decoction of Da-Huang-Xiao-Shi decoction was required while simplified one-step decoction was used in recent repsorts. To investigate the chemical difference between the decoctions obtained by the traditional and simplified preparations, a sensitive and reliable approach of high-performance liquid chromatography coupled with diode-array detection and electrospray ionization time-of-flight mass spectrometry was established. As a result, a total of 105 compounds were detected and identified. Analysis of the chromatogram profiles of the two decoctions showed that many compounds in the decoction of simplified preparation had changed obviously compared with those in traditional preparation. The changes of constituents would be bound to cause the differences in the therapeutic effects of the two decoctions. The present study demonstrated that certain preparation methods significantly affect the holistic quality of traditional Chinese medicines and the use of a suitable preparation method is crucial for these medicines to produce special clinical curative effect. This research results elucidated the scientific basis of traditional preparation methods in Chinese medicines. PMID:26914461

  12. Study of Intermediate Products from the Cross-linking Reactions of Genipin with Beta-Lactoglobulin and Related Peptides by MALDI-TOF/TOF Mass Spectrometry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Genipin, methyl-1R,2R,6S-2-hydroxy-9-(hydroxymethyl)-3-oxabicyclo[4.3.0]nona-4,8-diene-5-carboxylate, is an aglycone derivative from geniposide found in the Gardenia jasminoides fruits, obtained through enzymatic hydrolysis with beta-glucosidase. Genipin has been shown to be capable of cross-linking...

  13. Sequencing EVC and EVC2 identifies mutations in two-thirds of Ellis-van Creveld syndrome patients.

    PubMed

    Tompson, Stuart W J; Ruiz-Perez, Victor L; Blair, Helen J; Barton, Stephanie; Navarro, Victoria; Robson, Joanne L; Wright, Michael J; Goodship, Judith A

    2007-01-01

    Ellis-van Creveld syndrome (EvC) is caused by mutations in EVC and EVC2, genes in a divergent orientation separated by only 2.6 kb. We systematically sought mutations in both genes in a panel of 65 affected individuals to assess the proportion of cases resulting from mutations in each gene. We PCR amplified and sequenced the coding exons of both genes. We investigated mutations that could affect splicing by in vitro splicing assays and cDNA analysis. We have identified EVC mutations in 20 cases (31%); in all of these we have detected the mutation on each allele. We have identified EVC2 mutations in 25 cases (38%); in 22 of these we have isolated a mutation on each allele. The majority of the mutations introduce a premature termination codon. We sequenced the region between the two genes in 10 of the 20 cases in which we had not identified a mutation in either gene, revealing only one SNP that was not a common polymorphism. As we have not identified mutations in either gene in 20 cases (31%) it is possible that there is further genetic heterogeneity.

  14. Disruption of sonic hedgehog signaling in Ellis-van Creveld dwarfism confers protection against bipolar affective disorder.

    PubMed

    Ginns, E I; Galdzicka, M; Elston, R C; Song, Y E; Paul, S M; Egeland, J A

    2015-10-01

    Ellis-van Creveld syndrome, an autosomal recessively inherited chondrodysplastic dwarfism, is frequent among Old Order Amish of Pennsylvania. Decades of longitudinal research on bipolar affective disorder (BPAD) revealed cosegregation of high numbers of EvC and Bipolar I (BPI) cases in several large Amish families descending from the same pioneer. Despite the high prevalence of both disorders in these families, no EvC individual has ever been reported with BPI. The proximity of the EVC gene to our previously reported chromosome 4p16 BPAD locus with protective alleles, coupled with detailed clinical observations that EvC and BPI do not occur in the same individuals, led us to hypothesize that the genetic defect causing EvC in the Amish confers protection from BPI. This hypothesis is supported by a significant negative association of these two disorders when contrasted with absence of disease (P=0.029, Fisher's exact test, two-sided, verified by permutation to estimate the null distribution of the test statistic). As homozygous Amish EVC mutations causing EvC dwarfism do so by disrupting sonic hedgehog (Shh) signaling, our data implicate Shh signaling in the underlying pathophysiology of BPAD. Understanding how disrupted Shh signaling protects against BPI could uncover variants in the Shh pathway that cause or increase risk for this and related mood disorders.

  15. The importance of oral-clinical findings for the correct diagnosis of Ellis-van Creveld syndrome.

    PubMed

    Pedro, Rafael Lima; Andrade, Lucia Helena Raymundo; Maia, Lucianne Cople

    2011-01-01

    Ellis-van Creveld syndrome (EVC), also known as chondroectodermal dysplasia, is a rare autosomal recessive disorder. It is characterized by a variable spectrum of clinical findings, and the most common tetrad is chondrodystrophy, polydactyly, ectodermal dysplasia, and cardiac defects. Among the oral findings, number anomalies, peg-shaped teeth, and alterations in soft tissues are noteworthy. The aim of this paper is to report a case in which the diagnosis of EVC was possible only with the oral findings of a dentist and to relate the dental treatment performed. A 2-year-old girl was brought for dental care due to a lack of teeth. Her condition was initially diagnosed as thanatophoric dysplasia by her pediatrician. After evaluation of the oral manifestations, the dentist referred her to a geneticist, with the suggestion of EVC. The dental report, together with the systemic manifestations, allowed the geneticist to confirm the EVC diagnosis. The necessary dental procedures were performed, and the patient, now age 5, is still monitored by a multidisciplinary team.

  16. Biosurfactant production by hydrocarbon-degrading Brevibacterium and Vibrio isolates from the sea pen Pteroeides spinosum (Ellis, 1764).

    PubMed

    Graziano, Marco; Rizzo, Carmen; Michaud, Luigi; Porporato, Erika Maria Diletta; De Domenico, Emilio; Spanò, Nunziacarla; Lo Giudice, Angelina

    2016-09-01

    Among filter-feeders, pennatulids are the most complex and polymorphic members of the cnidarian class Anthozoa. They display a wide distribution throughout all the oceans, constituting a significant component of the sessile megafauna from intertidal to abyssal depths. In this study, a total of 118 bacterial isolates from enrichment cultures, carried out with homogenates of the sea pen Pteroeides spinosum (Ellis, 1764), were screened for hydrocarbon utilization by using the 2,6-dichlorophenol indophenol assay. Among them, 83 hydrocarbon-oxidizing isolates were analyzed for biosurfactant production by standard screening tests (i.e., emulsifying activity, E24 detection, surface tension measurement, microplate assay). The 16S rRNA gene sequencing revealed the affiliation of the most promising isolates to the genera Brevibacterium and Vibrio. Biosurfactant production resulted strongly affected by salinity and temperature conditions, and occurred in the presence of diesel oil and/or crude oil, whereas no production was observed when isolates were grown on tetradecane. The strains resulted able to create stable emulsions, thus suggesting the production of biosurfactants. Further analyses revealed a glycolipidic nature of the biosurfactant extracted from Vibrio sp. PBN295, a genus that has been only recently reported as biosurfactant producer. Results suggest that pennatulids could represent a novel source for the isolation of hydrocarbon-oxidizing bacteria with potential in biosurfactant production. PMID:27119461

  17. Differentiating the gum resins of two closely related Indian Gardenia species, G. gummifera and G. lucida, and establishing the source of dikamali gum resin using high-performance thin-layer chromatography and ultra-performance liquid chromatography-UV/MS.

    PubMed

    Lakavath, Suryanarayana; Avula, Bharathi; Wang, Yan-Hong; Rumalla, Chidananda Swamy; Gandhe, Sreekanth; Venkatrao, Adavi Rao Belvotagi; Satishchandra, Prabhakar Achanta; Bobbala, Ravi Kumar; Khan, Ikhlas A; Narasimha, Appa Rao Achanta Venkata

    2012-01-01

    Dikamali is a gum resin obtained from the leaf buds of Gardenia lucida or G. gummifera. There is controversy regarding the botanical source of this gum resin with some stating it to be from G. lucida while others claim it to be from G. gummifera. Analytical methods including UPLC and HPTLC were developed for the qualitative analysis of Gardenia species and various commercial samples. The separation using a UPLC method was achieved within 12.0 min by using C18 column material, a water/acetonitrile mobile phase, both containing formic acid, a gradient system, and a temperature of 40 degrees C. Extensive studies of dikamali collected from various parts of India in comparison with the gum resins collected from G. lucida and G. gummifera clearly indicated that the botanical source of commercially available dikamali is G. lucida, not G. gummifera. The marker compounds isolated from a market sample of dikamali were present only in the gum resin of G. lucida and the compounds isolated from G. gummifera were not present in any of the dikamali samples, confirming the botanical source of dikamali. This work is of utmost importance, given the ambiguity regarding the botanical source of the gum resin dikamali. LC/MS coupled with electrospray ionization is described for the identification and confirmation of nine compounds from various samples of the gum resin. An HPTLC method was also developed for the fast chemical fingerprint analysis of Gardenia samples.

  18. Truncation and microdeletion of EVC/EVC2 with missense mutation of EFCAB7 in Ellis-van Creveld syndrome.

    PubMed

    Nguyen, Tran Quynh Nhu; Saitoh, Makiko; Trinh, Huu Tung; Doan, Nguyen Minh Thien; Mizuno, Yoko; Seki, Masafumi; Sato, Yusuke; Ogawa, Seishi; Mizuguchi, Masashi

    2016-09-01

    Ellis-van Creveld syndrome (EvC) is a ciliopathy with cardiac anomalies, disproportionate short stature, polydactyly, dystrophic nails and oral defects. To obtain further insight into the genetics of EvC, we screened EVC/EVC2 mutations in eight Vietnamese EvC patients. All the patients had a congenital heart defect with atypical oral and/or skeletal abnormalities. One had compound heterozygous EVC2 mutations: a novel mutation c.769G > T-p.E177X in exon 6 inherited from father and another previously reported c.2476C > T-p.R826X mutation in exon 14 inherited from mother. The EVC2 mRNA expression level was significantly lower in the patient and her parents compared to controls. Another case had a novel heterozygous EVC mutation (c.1717C > G-p.S572X) in exon 12, inherited from his father. Of note, the mother without any EVC mutation on Sanger sequencing showed a lower expression level of EVC mRNA compared with controls. SNP array analysis revealed that the patient and mother had a heterozygous 16.4 kb deletion in EVC. This patient also had a heterozygous novel variant in exon 9 of EFCAB7 (c.1171 T > C-p.Y391H), inherited from his father. The atypical cardiac phenotype of this patient and the father suggested that EFCAB7 may modify the phenotype by interacting with EVC. In conclusion, we detected two novel nonsense mutations and a partial deletion of EVC/EVC2 in two Vietnamese families with EvC. Moreover, we found in one family a missense mutation of EFCAB7, a possible modifier gene in EvC and its related disorders.

  19. OBSERVATIONAL UPPER BOUND ON THE COSMIC ABUNDANCES OF NEGATIVE-MASS COMPACT OBJECTS AND ELLIS WORMHOLES FROM THE SLOAN DIGITAL SKY SURVEY QUASAR LENS SEARCH

    SciTech Connect

    Takahashi, Ryuichi; Asada, Hideki

    2013-05-01

    The latest result in the Sloan Digital Sky Survey Quasar Lens Search (SQLS) has set the first cosmological constraints on negative-mass compact objects and Ellis wormholes. There are no multiple images lensed by the above two exotic objects for {approx}50, 000 distant quasars in the SQLS data. Therefore, an upper bound is put on the cosmic abundances of these lenses. The number density of negative-mass compact objects is n < 10{sup -8}(10{sup -4}) h {sup 3} Mpc{sup -3} at the mass scale |M| > 10{sup 15}(10{sup 12}) M{sub Sun }, which corresponds to the cosmological density parameter |{Omega}| < 10{sup -4} at the galaxy and cluster mass range |M| = 10{sup 12-15} M{sub Sun }. The number density of the Ellis wormhole is n < 10{sup -4} h {sup 3} Mpc{sup -3} for a range of the throat radius a = 10-10{sup 4} pc, which is much smaller than the Einstein ring radius.

  20. Ellis v. Sherman.

    PubMed

    1984-06-29

    The Pennsylvania Superior Court upheld the Court of Common Pleas, Dauphin County, in its dismissal of a claim by the parents of a disabled child for "wrongful life." The infant was born with neurofibromatosis after the pediatrician, although informed before the child's conception that the father had a history of skin disease, failed to investigate its possible genetic implications. The Superior Court had ruled previously that claims for wrongful life would not be recognized in Pennsylvania due to the impossibility of calculating damages based on a comparison of impaired life with nonexistence.

  1. Novel homozygous mutations in the EVC and EVC2 genes in two consanguineous families segregating autosomal recessive Ellis-van Creveld syndrome.

    PubMed

    Aziz, Abdul; Raza, Syed I; Ali, Salman; Ahmad, Wasim

    2016-01-01

    Ellis-van Creveld syndrome (EVC) is a rare developmental disorder characterized by short limbs, short ribs, postaxial polydactyly, dysplastic nails, teeth, oral and cardiac abnormalities. It is caused by biallelic mutations in the EVC or EVC2 gene, separated by 2.6 kb of genomic sequence on chromosome 4p16. In the present study, we have investigated two consanguineous families of Pakistani origin, segregating EVC in autosomal recessive manner. Linkage in the families was established to chromosome 4p16. Subsequently, sequence analysis identified a novel nonsense mutation (p.Trp234*) in exon 8 of the EVC2 gene and 15 bp duplication in exon 14 of the EVC gene in the two families. This further expands the mutations in the EVC or EVC2 genes resulting in the EVC syndrome.

  2. Geniposide plays an anti-inflammatory role via regulating TLR4 and downstream signaling pathways in lipopolysaccharide-induced mastitis in mice.

    PubMed

    Song, Xiaojing; Zhang, Wen; Wang, Tiancheng; Jiang, Haichao; Zhang, Zecai; Fu, Yunhe; Yang, Zhengtao; Cao, Yongguo; Zhang, Naisheng

    2014-10-01

    Geniposide is a medicine isolated from Gardenia jasminoides Ellis, which is a traditional Chinese herb that is widely used in Asia for the treatment of inflammation, brain diseases, and hepatic disorders. Mastitis is a highly prevalent and important infectious disease. In this study, we used a lipopolysaccharide (LPS)-induced mouse mastitis model and LPS-stimulated primary mouse mammary epithelial cells (mMECs) to explore the anti-inflammatory effect and the mechanism of action of geniposide. Using intraductal injection of LPS as a mouse model of mastitis, we found that geniposide significantly reduced the infiltration of inflammatory cells and downregulated the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). To further investigate the anti-inflammatory mechanism, we used LPS-stimulated mMECs as an in vitro mastitis model. The results of enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR) showed that geniposide inhibited the expression of TNF-α, IL-1β, and IL-6 in a dose-dependent manner. Western blot analysis demonstrated that geniposide could suppress the phosphorylation of inhibitory kappa B (IκBα), nuclear factor-κB (NF-κB), p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). Geniposide also inhibited the expression of toll-like receptor 4 (TLR4) in the LPS-stimulated mMECs. In conclusion, geniposide exerted its anti-inflammatory effect by regulating TLR4 expression, which affected the downstream NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways. Thus, geniposide may be a potential drug for mastitis therapy.

  3. Characterization of chemical constituents in Zhi-Zi-Da-Huang decoction by ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.

    PubMed

    Zhu, Heyun; Yin, Ran; Han, Fei; Guan, Jiao; Zhang, Xiaoshu; Mao, Xinjuan; Zhao, Longshan; Li, Qing; Hou, Xiaohong; Bi, Kaishun

    2014-12-01

    A sensitive and reliable ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established to separate and identify the chemical constituents of Zhi-Zi-Da-Huang decoction, a classic traditional Chinese medicine formula. The chromatographic separation was achieved on a Shim-pack XR-ODS C18 column (75 × 3.0 mm, 2.2 μm) using a gradient elution program. The detection was performed on a Waters Xevo G2 Q-TOF mass spectrometer equipped with electrospray ionization source in both positive and negative modes. With the optimized conditions, a total of 82 compounds were identified or tentatively characterized. Of the 82 compounds, 21 compounds were identified by comparing the retention time and MS data with reference standards, the rest were characterized by analyzing MS data and retrieving the reference literature. In addition, 31 compounds were identified from Gardenia jasminoides Ellis, ten compounds were identified from Rheum palmatum L., 33 compounds were identified from Citrus aurantium L., and eight compounds were identified from Sojae Semen Praeparatum. Results indicated that iridoids, anthraquinones, flavonoids, isoflavonoids, coumarins, glycosides of crocetin, monoterpenoids, and organic acids were major constituents in Zhi-Zi-Da-Huang decoction. It is concluded that the developed ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method with high sensitivity and resolution is suitable for identifying and characterizing the chemical constituents of Zhi-Zi-Da-Huang decoction, and the analysis provides a helpful chemical basis for further research on Zhi-Zi-Da-Huang decoction. PMID:25195935

  4. Genipin protects lipopolysaccharide-induced apoptotic liver damage in D-galactosamine-sensitized mice.

    PubMed

    Kim, Seok-Joo; Kim, Joon-Ki; Lee, Dong-Ung; Kwak, Jong-Hwan; Lee, Sun-Mee

    2010-06-10

    This study examined the effects of genipin, isolated from Gardenia jasminoides Ellis, on d-galactosamine (GalN) and lipopolysaccharide (LPS)-induced hepatic apoptosis and liver failure. Mice were given an intraperitoneal injection of genipin (25, 50, 100 and 200mg/kg) 1h before GalN (700mg/kg)/LPS (10microg/kg) administration. The survival rate of the genipin group was significantly higher than that of the control. Genipin markedly reduced the increases in serum aminotransferase activities and lipid peroxidation. The glutathione content decreased in GalN/LPS group, and this decrease was attenuated by genipin. Increases in serum tumor necrosis factor-alpha (TNF-alpha), which were observed in GalN/LPS-treated mice, were significantly reduced by genipin. Genipin attenuated the GalN/LPS-induced apoptosis of hepatocytes, as estimated by the caspase-3 and -8 activity assay, TNF-R1 associated death domain (TRADD) protein measurement and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method. Moreover, increased cytosolic cytochrome c protein was reduced by genipin. After 3h of GalN/LPS injection, nuclear phosphorylated c-Jun (p-c-Jun) level was significantly increased, whereas it was attenuated by genipin. Also, the increased nuclear level of nuclear factor-kappaB and the decreased cytosolic level of IkappaB-alpha protein were significantly attenuated by genipin. Our results suggest that genipin offers marked hepatoprotection against damage induced by GalN/LPS related with its antioxidative, anti-apoptotic activities, and inhibition of NF-kappaB nuclear translocation and nuclear p-c-Jun expression. PMID:20303938

  5. Effects and mechanisms of Geniposide on rats with adjuvant arthritis.

    PubMed

    Dai, Miao-Miao; Wu, Hong; Li, Hui; Chen, Jian; Chen, Jin-Yun; Hu, Shun-Li; Shen, Chen

    2014-05-01

    Geniposide (GE), an iridoid glycoside compound, is the major active ingredient of Gardenia jasminoides Ellis (GJ) fruit which has anti-inflammatory and other important therapeutic activities. The aim of this study was to investigate the effects of GE on adjuvant arthritis (AA) rats and its possible mechanisms. AA was induced by injecting with Freund's complete adjuvant (FCA). Male SD rats were subjected to treatment with GE at 30, 60 and 120mg/kg from days 18 to 24 after immunization. Lymphocyte proliferation was assessed by MTT. Interleukin (IL)-6, IL-17, IL-4 and transforming growth factor-beta 1 (TGF-β1) were determined by ELISA. c-Jun N-terminal kinase (JNK) and phospho-JNK (p-JNK) were detected by Western blot. GE (60, 120mg/kg) significantly relieved the secondary hind paw swelling and arthritis index, along with decreased Th17-cells cytokines and increased Treg-cell cytokines in mesenteric lymph node lymphocytes (MLNL) and peripheral blood lymphocytes (PBL) of AA rats. In addition, GE decreased the expression of p-JNK in MLNL and PBL of AA rats. In vivo study, it was also observed that GE attenuated histopathologic changes of MLN in AA rats. Collectively, GE might exert its anti-inflammatory and immunoregulatory effects through inducing Th17 cell immune tolerance and enhancing Treg cell-mediated activities by down-regulating the expression of p-JNK. The mechanisms of GE on JNK signaling in MLNL and PBL may play critical roles in the pathogenesis of rheumatoid arthritis. PMID:24583144

  6. Identification of the absorbed components and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma by ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry.

    PubMed

    Zhu, Heyun; Bi, Kaishun; Han, Fei; Guan, Jiao; Zhang, Xiaoshu; Mao, Xinjuan; Zhao, Longshan; Li, Qing; Hou, Xiaohong; Yin, Ran

    2015-01-01

    Zhi-Zi-Da-Huang decoction (ZZDHD), consisting of Gardenia jasminoides Ellis, Rheum palmatum L., Citrus aurantium L. and Sojae Semen Praeparatum, is a widely used traditional Chinese medicine preparation for the treatment of acute or chronic hepatic diseases. In the present study, a sensitive and selective ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was developed to separate and identify the absorbed components and metabolites in rat plasma after oral administration of ZZDHD. The plasma samples were pretreated by protein precipitation and separated on a Shim-pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) using a gradient elution program. Mass spectrometric detection was performed on an Agilent 6520 Q-TOF mass spectrometer equipped with electrospray ionization (ESI) source in positive and negative ion modes. By comparing the retention time, high resolution mass data of blank plasma and dosed plasma, a total of 43 constituents, including 21 prototype compounds and 22 metabolites were identified or tentatively characterized. Results indicated that glucuronidation and sulfation were the main metabolic pathways of iridoid glycosides and anthraquinones, glucuronidation was the main metabolic pathways of flavanone-related compounds. It is concluded the developed UHPLC-Q-TOF-MS method with high sensitivity and resolution is suitable for identifying and characterizing the absorbed components and metabolites of ZZDHD, and the results will provide essential data for further studying the relationship between the chemical components and pharmacological activity of ZZDHD. PMID:25912849

  7. Genipin ameliorates age-related insulin resistance through inhibiting hepatic oxidative stress and mitochondrial dysfunction.

    PubMed

    Guan, Lili; Feng, Haiyan; Gong, Dezheng; Zhao, Xu; Cai, Li; Wu, Qiong; Yuan, Bo; Yang, Mei; Zhao, Jie; Zou, Yuan

    2013-12-01

    Insulin resistance (IR) increases with age and plays a key role in the pathogenesis of type 2 diabetes mellitus. Oxidative stress and mitochondrial dysfunction are supposed to be major factors leading to age-related IR. Genipin, an extract from Gardenia jasminoides Ellis fruit, has been reported to stimulate insulin secretion in pancreatic islet cells by regulating mitochondrial function. In this study, we first investigated the effects of genipin on insulin sensitivity and the potential mitochondrial mechanisms in the liver of aging rats. The rats were randomly assigned to receive intraperitoneal injections of either 25mg/kg genipin or vehicle once daily for 12days. The aging rats showed hyperinsulinemia and hyperlipidemia, and insulin resistance as examined by the decreased glucose decay constant rate during insulin tolerance test (kITT). The hepatic tissues showed steatosis and reduced glycogen content. Hepatic malondialdehyde level and mitochondrial reactive oxygen species (ROS) were higher, and levels of mitochondrial membrane potential (MMP) and ATP were lower as compared with the normal control rats. Administration of genipin ameliorated systemic and hepatic insulin resistance, alleviated hyperinsulinemia, hyperglyceridemia and hepatic steatosis, relieved hepatic oxidative stress and mitochondrial dysfunction in aging rats. Furthermore, genipin not only improved insulin sensitivity by promoting insulin-stimulated glucose consumption and glycogen synthesis, inhibited cellular ROS overproduction and alleviated the reduction of levels of MMP and ATP, but also reversed oxidative stress-associated JNK hyperactivation and reduced Akt phosphorylation in palmitate-treated L02 hepatocytes. In conclusion, genipin ameliorates age-related insulin resistance through inhibiting hepatic oxidative stress and mitochondrial dysfunction. PMID:24041487

  8. The pharmacokinetic profile of crocetin in healthy adult human volunteers after a single oral administration.

    PubMed

    Umigai, N; Murakami, K; Ulit, M V; Antonio, L S; Shirotori, M; Morikawa, H; Nakano, T

    2011-05-15

    Crocetin, a unique carotenoid with a short carbon chain length, is an active compound of saffron and Gardenia jasminoides Ellis used as traditional herbal medicine. The present study was undertaken to investigate the pharmacokinetic profiles of crocetin in healthy adult subjects. The study was conducted as an open-label, single dose escalation with 10 Filipino volunteers (5 men and 5 women). The subjects received a single dose of crocetin at three doses (7.5, 15 and 22.5 mg) in one week interval. Blood samples were collected from the brachial vein before and at 1, 2, 4, 6, 8, 10 and 24 h after administration. Plasma concentrations of crocetin were determined by high-performance liquid chromatography (HPLC). Crocetin was rapidly absorbed and detected within an hour of administration with a mean time to reach maximum concentration (T(max)) of crocetin ranging from 4.0 to 4.8 h. The mean values of C(max) and AUC(0-24h) ranged from 100.9 to 279.7 ng/ml and 556.5 to 1720.8 ng. h/ml respectively. C(max) and AUC values increased with dose proportional manner. Crocetin was eliminated from human plasma with a mean elimination half life (T(½) of 6.1 to 7.5 h. In summary, there were no serious adverse events up to 22.5 mg dose of crocetin while crocetin was found to be absorbed more quickly than the other carotenoids such as β-carotene, lutein and lycopene. PMID:21112749

  9. Preventive effect of phytoglycoprotein (27 kDa) on inflammatory factors at liver injury in cadmium chloride-exposed ICR mice.

    PubMed

    Lee, Jin; Lim, Kye-Taek

    2011-02-01

    Cadmium is one of the inflammation-related xenobiotics and has been regarded as a potent carcinogen. Gardenia jasminoides Ellis (GJE) has been used to cure inflammation in Korean folk medicine for a long time. The purpose of present study is the inhibitory effect of glycoprotein isolated from GJE (27 kDa) on inflammation mechanism in cadmium chloride-exposed ICR mice. We evaluated the activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), and thiobarbituric acid-reactive substances (TBARS), activities of anti-oxidative enzymes [superoxide dismutase (SOD) and gluthathione peroxidase (GPx)], activities of c-Jun N-terminal protein kinase (JNK), heat shock protein 27 (Hsp27), activator protein (AP)-1, nuclear factor (NF)-κB and expression of inflammation-related mediators including tumor necrosis factor (TNF)-α and interleukin (IL)-6 in cadmium chloride-exposed ICR mice using immunoblot analysis, EMSA and RT-PCR. It notes that mice plasma was used to measure ALT, LDH, and TBARS after treatment with cadmium chloride alone or cadmium chloride under the pretreatment with GJE glycoprotein. Liver tissues were used to assess activities of anti-oxidant enzymes, SAPK/JNK, Hsp27, AP-1, NF-κB, TNF-α, and IL-6 in this study. The results obtained from this study revealed that GJE glycoprotein (10 mg/kg) decreased the levels of LDH, ALT and TBARS, whereas increased the activity of hepatic anti-oxidant enzymes (SOD and GPx) in cadmium chloride-exposed ICR mice. Moreover, it decreased the activity of JNK/AP-1, NF-κB, Hsp27, and pro-inflammatory cytokines (TNF-α and IL-6). Taken together, the results in this study suggest that GJE glycoprotein inhibits the expression of inflammation-related cytokines (TNF-α and IL-6) in cadmium chloride-exposed ICR mice. PMID:21268091

  10. Preventive effect of geniposide on metabolic disease status in spontaneously obese type 2 diabetic mice and free fatty acid-treated HepG2 cells.

    PubMed

    Kojima, Kazuko; Shimada, Tsutomu; Nagareda, Yasuhiro; Watanabe, Michiru; Ishizaki, Junko; Sai, Yoshimichi; Miyamoto, Ken-ichi; Aburada, Masaki

    2011-01-01

    Accumulation of visceral fat induces various symptoms of metabolic syndrome such as insulin resistance and abnormal glucose/lipid metabolism and eventually leads to the onset of ischemic cerebrovascular diseases. Geniposide, which is iridoid glycoside from the fruit of Gardenia jasminoides ELLIS, is recognized as being useful against hyperlipidemia and fatty liver. In order to clarify the effect of geniposide on metabolic disease-based visceral fat accumulation and the relevant molecular mechanism, experiments were performed in spontaneously obese Type 2 diabetic TSOD mice and the free fatty acid-treated HepG2 cells. In the TSOD mice, geniposide showed suppression of body weight and visceral fat accumulation, alleviation of abnormal lipid metabolism and suppression of intrahepatic lipid accumulation. In addition, geniposide alleviated abnormal glucose tolerance and hyperinsulinemia, suggesting that geniposide has an insulin resistance-alleviating effect. Next, in order to investigate the direct effect of geniposide on the liver, the effect on the free fatty acid-treated HepG2 fatty liver model was investigated using genipin, which is the aglycone portion of geniposide. Genipin suppressed the intracellular lipid accumulation caused by the free fatty acid treatment and also significantly increased the intracellular expression of a fatty acid oxidation-related gene (peroxisomal proliferator-activated receptor: PPARα). From these results, it was confirmed that geniposide has an anti-obesity effect, an insulin resistance-alleviating effect and an abnormal lipid metabolism-alleviating effect, and the metabolite genipin shows a direct effect on the liver, inducing expression of a lipid metabolism-related gene as one of its molecular mechanisms. PMID:21963504

  11. Geniposide plays an anti-inflammatory role via regulating TLR4 and downstream signaling pathways in lipopolysaccharide-induced mastitis in mice.

    PubMed

    Song, Xiaojing; Zhang, Wen; Wang, Tiancheng; Jiang, Haichao; Zhang, Zecai; Fu, Yunhe; Yang, Zhengtao; Cao, Yongguo; Zhang, Naisheng

    2014-10-01

    Geniposide is a medicine isolated from Gardenia jasminoides Ellis, which is a traditional Chinese herb that is widely used in Asia for the treatment of inflammation, brain diseases, and hepatic disorders. Mastitis is a highly prevalent and important infectious disease. In this study, we used a lipopolysaccharide (LPS)-induced mouse mastitis model and LPS-stimulated primary mouse mammary epithelial cells (mMECs) to explore the anti-inflammatory effect and the mechanism of action of geniposide. Using intraductal injection of LPS as a mouse model of mastitis, we found that geniposide significantly reduced the infiltration of inflammatory cells and downregulated the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). To further investigate the anti-inflammatory mechanism, we used LPS-stimulated mMECs as an in vitro mastitis model. The results of enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR) showed that geniposide inhibited the expression of TNF-α, IL-1β, and IL-6 in a dose-dependent manner. Western blot analysis demonstrated that geniposide could suppress the phosphorylation of inhibitory kappa B (IκBα), nuclear factor-κB (NF-κB), p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). Geniposide also inhibited the expression of toll-like receptor 4 (TLR4) in the LPS-stimulated mMECs. In conclusion, geniposide exerted its anti-inflammatory effect by regulating TLR4 expression, which affected the downstream NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways. Thus, geniposide may be a potential drug for mastitis therapy. PMID:24771071

  12. Geniposide alleviates inflammation by suppressing MeCP2 in mice with carbon tetrachloride-induced acute liver injury and LPS-treated THP-1 cells.

    PubMed

    Ma, Tao-tao; Li, Xiao-feng; Li, Wan-xia; Yang, Yang; Huang, Cheng; Meng, Xiao-ming; Zhang, Lei; Li, Jun

    2015-12-01

    Geniposide (GP), an iridoid glucoside extracted from Gardenia jasminoides Ellis fruits, has been used as a herbal medicine to treat liver and gall bladder disorders for many years. However the mechanism of anti-inflammatory is largely unknown. In this study, GP significantly attenuated inflammation in acute liver injury (ALI) mice model and in lipopolysaccharide (LPS)-induced THP-1 cells. It was demonstrated that GP obviously decreased the expression of Methyl-CpG binding protein 2 (MeCP2) in vivo and in vitro. Knockdown of MeCP2 with siRNA suppressed the expressions of IL-6 and TNF-α, while over-expression of MeCP2 had a proinflammatory effect on the expression of IL-6 and TNF-α in LPS-induced THP-1 cells. Mechanistically, it was indicated that GP had anti-inflammatory effects at least in part, through suppressing MeCP2. Interestingly, GP could attenuate expressions of Sonic hedgehog (Shh) and GLIS family zinc finger 1 (GLIS1) but increase Ptched1 (PTCH1) expression. Similar findings were also demonstrated at the protein level by siRNA MeCP2. Furthermore, over-expression of MeCP2 obviously increased Shh and GLIS1 expressions but reduced PTCH1 expression. Taken together, GP may serve as an effective modulator of MeCP2-hedgehog pathway (Hh)-axis during the pathogenesis of inflammation. Our findings shed light on the potential therapeutic feature of GP in recovering inflammatory diseases. PMID:26371859

  13. Antidepressant-like effect of geniposide on chronic unpredictable mild stress-induced depressive rats by regulating the hypothalamus-pituitary-adrenal axis.

    PubMed

    Cai, Li; Li, Rong; Tang, Wen-jian; Meng, Gang; Hu, Xiang-yang; Wu, Ting-ni

    2015-08-01

    Geniposide as the major active component of Gardenia jasminoides Ellis has neuroprotective activity. This study elucidated the potential antidepressant-like effect of geniposide and its related mechanisms using a depression rat model induced by 3 consecutive weeks of chronic unpredictable mild stress (CUMS). Sucrose preference test, open field test (OFT) and forced swimming test (FST) were applied to evaluate the antidepressant effect of geniposide. Adrenocorticotropic hormone (ACTH) and corticosterone (CORT) serum levels, adrenal gland index and hypothalamic corticotrophin-releasing hormone (CRH) mRNA expression were measured to assess the activity of hypothalamus-pituitary-adrenal (HPA) axis. Hypothalamic glucocorticoid receptor α (GRα) mRNA expression and GRα protein expression in hypothalamic paraventricular nucleus (PVN) were also determined by real-time PCR and immunohistochemistry, respectively. We found that geniposide (25, 50, 100mg/kg) treatment reversed the CUMS-induced behavioral abnormalities, as suggested by increased sucrose intake, improved crossing and rearing behavior in OFT, shortened immobility and prolonged swimming time in FST. Additionally, geniposide treatment normalized the CUMS-induced hyperactivity of HPA axis, as evidenced by reduced CORT serum level, adrenal gland index and hypothalamic CRH mRNA expression, with no significant effect on ACTH serum level. Moreover, geniposide treatment upregulated the hypothalamic GRα mRNA level and GRα protein expression in PVN, suggesting geniposide could recover the impaired GRα negative feedback on CRH expression and HPA axis. These aforementioned therapeutic effects of geniposide were essentially similar to fluoxetine. Our results indicated that geniposide possessed potent antidepressant-like properties that may be mediated by its effects on the HPA axis. PMID:25914157

  14. An untargeted metabolomics-driven approach based on LC-TOF/MS and LC-MS/MS for the screening of xenobiotics and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma.

    PubMed

    Wu, Huan; Li, Xixi; Yan, Xuemei; An, Li; Luo, Kaiwen; Shao, Mingjing; Jiang, Yue; Xie, Rui; Feng, Fang

    2015-11-10

    Zhi-Zi-Da-Huang decoction (ZZDHD), a typical traditional Chinese medicine prescription, is widely used in clinical practice for the treatment of alcoholic liver disease. However, due to lack of holistic metabolic research, the active ingredients of ZZDHD have not been fully elucidated. It entails a huge obstacle for the quality evaluation, pharmacokinetic studies and clinical-safe medication administration of ZZDHD. In this work, an untargeted metabolomics-driven approach was proposed to rapidly screen and characterize xenobiotics and related metabolites in vivo conducted by LC-TOF/MS and LC-QqQ/MS. The tR-m/z pairs which were present in the ZZDHD-dosed group and absent in the control group could be clearly displayed by XCMS Online platform combined with supervised orthogonal partial least squares discriminant analysis. Among them, a total of 61 ZZDHD-related xenobiotics and metabolites including 34 prototype components and 27 metabolites were rapidly identified or tentatively characterized in rat plasma. The results indicated that iridoid glycosides and monoterpenoids from Gardenia jasminoides Ellis, flavonoid glycosides from Citrus aurantium L., as well as anthraquinones from Rheum palmatum L. were the main absorbed chemical components of ZZDHD. Hydrolysis, glucuronidation and sulfation were the main metabolic pathways of ZZDHD in vivo. The present study provided a solid basis for further revealing the relationship between the xenobiotic metabolome and pharmacological activity of ZZDHD. In addition, the application of untargeted metabolomics-driven approach offers a fresh insight for rapid screening and identifying xenobiotics and metabolites of ZZDHD and other multiherb prescription. PMID:26275719

  15. Characterization of chemical constituents in Zhi-Zi-Da-Huang decoction by ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.

    PubMed

    Zhu, Heyun; Yin, Ran; Han, Fei; Guan, Jiao; Zhang, Xiaoshu; Mao, Xinjuan; Zhao, Longshan; Li, Qing; Hou, Xiaohong; Bi, Kaishun

    2014-12-01

    A sensitive and reliable ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established to separate and identify the chemical constituents of Zhi-Zi-Da-Huang decoction, a classic traditional Chinese medicine formula. The chromatographic separation was achieved on a Shim-pack XR-ODS C18 column (75 × 3.0 mm, 2.2 μm) using a gradient elution program. The detection was performed on a Waters Xevo G2 Q-TOF mass spectrometer equipped with electrospray ionization source in both positive and negative modes. With the optimized conditions, a total of 82 compounds were identified or tentatively characterized. Of the 82 compounds, 21 compounds were identified by comparing the retention time and MS data with reference standards, the rest were characterized by analyzing MS data and retrieving the reference literature. In addition, 31 compounds were identified from Gardenia jasminoides Ellis, ten compounds were identified from Rheum palmatum L., 33 compounds were identified from Citrus aurantium L., and eight compounds were identified from Sojae Semen Praeparatum. Results indicated that iridoids, anthraquinones, flavonoids, isoflavonoids, coumarins, glycosides of crocetin, monoterpenoids, and organic acids were major constituents in Zhi-Zi-Da-Huang decoction. It is concluded that the developed ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method with high sensitivity and resolution is suitable for identifying and characterizing the chemical constituents of Zhi-Zi-Da-Huang decoction, and the analysis provides a helpful chemical basis for further research on Zhi-Zi-Da-Huang decoction.

  16. Ellis-van Creveld syndrome in a fetus with rhizomelia and polydactyly. Report of a case diagnosed by genetic analysis, and correlation with pathological andradiologic findings.

    PubMed

    Peraita-Ezcurra, Milena; Martínez-García, Mónica; Ruiz-Pérez, Víctor L; Sánchez-Gutiérrez, María Eugenia; Fenollar-Cortés, María; Vélez-Monsalve, Camilo; Ramos-Corrales, Carmen; Pastor, Ignacio; Santonja, Carlos; Trujillo-Tiebas, María José

    2012-05-10

    Ellis-van Creveld syndrome is an autosomal recessive disorder mainly characterized by a disproportionate limb dwarfism, chondroectodermal dysplasia, congenital heart disease, postaxial polydactyly, and dysplastic fingernails and teeth. Only 300 cases have been published worldwide. We report a 21-week fetus with rhizomelia and polydactyly detected. Gross photographs, radiologic studies and pathological study were performed leading to the clinico-pathological suspicion of EvC. DNA from fresh fetal tissue was extracted for sequencing the EVC and EVC2 genes. p.W215X and p.R677X mutations were identified in the EVC2 gene in the fetal sample. Parental sample analysis showed the p.W215X mutation to be inherited from the mother and the p.R677X mutation from the father. The clinical information is essential not only to arrive at a correct diagnosis in fetuses with pathologic ultrasound findings, but also to offer a proper genetic counseling to the parents and their relatives.

  17. Exclusion of the Ellis-van Creveld region on chromosome 4p16 in some families with asphyxiating thoracic dystrophy and short-rib polydactyly syndromes.

    PubMed

    Krakow, D; Salazar, D; Wilcox, W R; Rimoin, D L; Cohn, D H

    2000-08-01

    Ellis-van Creveld syndrome (EVC) is a relatively rare, usually non-lethal, autosomal recessive skeletal dysplasia characterized by short stature, polydactyly, cardiac and renal anomalies. Linkage analysis has localized the disease gene to chromosome 4p16, with the markers at loci D4S827 and D4S3135 defining the centromeric and telomeric limits of the linked interval, respectively. There has been long-term speculation that asphyxiating thoracic dystrophy (ATD) and the short-rib polydactyly syndromes (SRP) represent the severe end of the EVC disease spectrum. We performed linkage analysis using markers from the EVC region in seven families manifesting either ATD or SRP type III. In two of the families, one segregating ATD and one SRP kindred, linkage of the phenotype to the EVC region was excluded. In the other five families linkage of the phenotype to the EVC region could not be excluded, but the families were too small for linkage to the region to be established. The exclusion of the EVC region in ATD and SRP III families suggests that locus heterogeneity exists within the short-rib dysplasia (with and without polydactyly) group of disorders.

  18. Two novel heterozygous mutations of EVC2 cause a mild phenotype of Ellis-van Creveld syndrome in a Chinese family.

    PubMed

    Shen, Wenjing; Han, Dong; Zhang, Jin; Zhao, Hongshan; Feng, Hailan

    2011-09-01

    Ellis-van Creveld syndrome (EvC, chondroectodermal dysplasia; OMIM 225500) is an autosomal recessive skeletal dysplasia with associated multisystem involvement. The syndrome is characterized by short limbs, short ribs, postaxial polydactyly, dysplastic nails, and abnormal teeth. Congenital heart defects occur in 50-60% of cases. In this study, we report EvC in a 6-year-old Chinese girl with hypodontia and polydactyly, mild short stature, and abnormalities of the knee joints. No signs of short ribs, narrow thorax, or congenital heart defects were found in this patient. The EvC phenotype shares some similarity with Weyers acrofacial dysostosis (Weyer; OMIM 193530), an autosomal dominant disorder clinically characterized by mild short stature, postaxial polydactyly, nail dystrophy, and dysplastic teeth. Mutations in EVC or EVC2 are associated with both EvC syndrome and Weyers acrodental dysostosis, but the two conditions differ in the severity of the phenotype and their pattern of inheritance. In this study, two novel heterozygous EVC2 mutations, IVS5-2A > G and c.2653C > T (Arg885X), were identified in the patient. The IVS5-2A > G mutation was inherited from the patient's mother and the c.2653C > T from her father. Her parents have no phenotypic symptoms similar to those of the patient. These findings extend the mutation spectrum of this malformation syndrome and provide the possibility of prenatal diagnosis for future offspring in this family.

  19. A Turkish family with Ellis-van Creveld syndrome in six siblings; linkage analysis on 4p16 region (D4S3360-D4S2366).

    PubMed

    Cağdaş, D N; Parlar, A I; Pac, A; Tutun, U; Balci, S

    2008-01-01

    We present a Turkish family and their 6 children, consecutively affected by Ellis-van Creveld (EVC) Syndrome. Four of the affected children died in the postnatal period, and 2 of them had been admitted to the pediatric cardiology department for their cardiologic evaluation. Since they had the features of the EVC Syndrome, linkage analysis was performed with the polymorphic markers, D4S3360-D4S2366, selected from 4p 16 locus. There was complete segregation between the disease and marker allels and the two affected siblings were homozygote for the polymorphic markers, as expected in autosomal recessive inheritance. The diagnosis of EVC Syndrome was confirmed by this molecular analysis. Two cases with EVC were presented in this report. Case 1 had partial abnormal pulmonary venous return and pulmonary stenosis additional to ostium primum atrial septal defect and mitral cleft. Partial abnormal pulmonary venous return and pulmonary stenosis were previously not reported with EVC Syndrome. Postaxial polydactyly phenotype of the Case 2 differs from her brother's. There is bifid 5th metacarpal and unilateral (L) bifid middle and distal phalanges resembling syndactyly.

  20. The influence of secretion elicitors and external pH on the kinetics of D-alanine uptake by the trap lobes of Dionaea muscipula Ellis (Venus's Flytrap).

    PubMed

    Rea, P A; Whatley, F R

    1983-08-01

    Simple kinetic techniques were used to examine the mechanism of D-alanine uptake by the adaxial surfaces of the trap lobes of Dionaea muscipula Ellis (Venus's Flytrap.) On the basis of these analyses, the uptake of D-alanine was found to depend on the time during which the trap lobes were inoculated with elicitors of secretion before excision and measurement of uptake. Disks taken from traps that had not been subjected to a preceding period of inoculation with secretion elicitors showed a low basal rate of uptake which was neither pH-dependent nor exhibited saturation with respect to external D-alanine concentration. Disks from preinoculated traps, on the other hand, displayed an enhanced rate of uptake which showed both pH-dependence and saturation with respect to external D-alanine concentration. The capacity for enhanced uptake was lost upon prolonged inoculation or when inoculation was stopped. Of the compounds tested, only elicitors of secretion caused an enhancement of uptake. The enhanced rate of D-alanine uptake is temperature-sensitive with a Q10 characteristic of a mediated process. Uncouplers cause an instantaneous abolition of uptake whereas the effects of terminal-oxidase inhibitors are time-dependent. The pH-dependence of uptake is inferred to result from an increased affinity of the carrier system for D-alanine at low pH values. Although the ionic state of D-alanine is relatively unaffected over the pH range examined, a decrease in the external pH from 6.0 to 3.8 decreases the apparent K m for uptake by four-fold but increases V max by only 30%. It is concluded that the acid secreted by the digestive glands of Dionaea plays a direct role in facilitating the uptake of amino acids from the trap cavity.

  1. Tanning Effects of Aluminum -Genipin or -Vegetable Tannin Combinations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Genipin, a naturally occurring protein crosslinking agent, isolated from the fruit of Gardenia jasmindides Ellis, is beginning to replace glutaraldehyde as a fixative for biological tissues. Earlier research in this laboratory demonstrated that when hide powder was first tanned with 8% aluminum and...

  2. Ellis-Van Creveld Dysplasia

    MedlinePlus

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  3. Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium.

    PubMed

    Caparrós-Martín, José A; De Luca, Alessandro; Cartault, François; Aglan, Mona; Temtamy, Samia; Otaify, Ghada A; Mehrez, Mennat; Valencia, María; Vázquez, Laura; Alessandri, Jean-Luc; Nevado, Julián; Rueda-Arenas, Inmaculada; Heath, Karen E; Digilio, Maria Cristina; Dallapiccola, Bruno; Goodship, Judith A; Mill, Pleasantine; Lapunzina, Pablo; Ruiz-Perez, Victor L

    2015-07-15

    Most patients with Ellis-van Creveld syndrome (EvC) are identified with pathogenic changes in EVC or EVC2, however further genetic heterogeneity has been suggested. In this report we describe pathogenic splicing variants in WDR35, encoding retrograde intraflagellar transport protein 121 (IFT121), in three families with a clinical diagnosis of EvC but having a distinctive phenotype. To understand why WDR35 variants result in EvC, we analysed EVC, EVC2 and Smoothened (SMO) in IFT-A deficient cells. We found that the three proteins failed to localize to Wdr35(-/-) cilia, but not to the cilium of the IFT retrograde motor mutant Dync2h1(-/-), indicating that IFT121 is specifically required for their entry into the ciliary compartment. Furthermore expression of Wdr35 disease cDNAs in Wdr35(-/-) fibroblasts revealed that the newly identified variants lead to Hedgehog signalling defects resembling those of Evc(-/-) and Evc2(-/-) mutants. Together our data indicate that splicing variants in WDR35, and possibly in other IFT-A components, underlie a number of EvC cases by disrupting targeting of both the EvC complex and SMO to cilia.

  4. Long interspersed nuclear element-1 (LINE1)-mediated deletion of EVC, EVC2, C4orf6, and STK32B in Ellis-van Creveld syndrome with borderline intelligence.

    PubMed

    Temtamy, Samia A; Aglan, Mona S; Valencia, Maria; Cocchi, Guido; Pacheco, Maria; Ashour, Adel M; Amr, Khalda S; Helmy, Sanaa M H; El-Gammal, Mona A; Wright, Michael; Lapunzina, Pablo; Goodship, Judith A; Ruiz-Perez, Victor L

    2008-07-01

    Previous work has shown Ellis-van Creveld (EvC) patients with mutations either in both alleles of EVC or in both alleles of EVC2. We now report affected individuals with the two genes inactivated on each allele. In a consanguineous pedigree diagnosed with EvC and borderline intelligence, we detected a 520-kb homozygous deletion comprising EVC, EVC2, C4orf6, and STK32B, caused by recombination between long interspersed nuclear element-1 (LINE-1 or L1) elements. Patients homozygous for the deletion are deficient in EVC and EVC2 and have no increase in the severity of the EvC typical features. Similarly deletion carriers demonstrate absence of digenic inheritance in EvC. Further, the phenotype of these patients suggests that the EVC-STK32B deletion also leads to mild mental retardation and reveals that loss of the novel genes C4orf6 and STK32B causes at most mild mental deficit. In an EvC compound heterozygote of different ethnic origin we identified the same LINE-to-LINE rearrangement due to a different recombination event. These findings highlight the importance of L1 repetitive sequences in human genome architecture and disease.

  5. Specific variants in WDR35 cause a distinctive form of Ellis-van Creveld syndrome by disrupting the recruitment of the EvC complex and SMO into the cilium

    PubMed Central

    Caparrós-Martín, José A.; De Luca, Alessandro; Cartault, François; Aglan, Mona; Temtamy, Samia; Otaify, Ghada A.; Mehrez, Mennat; Valencia, María; Vázquez, Laura; Alessandri, Jean-Luc; Nevado, Julián; Rueda-Arenas, Inmaculada; Heath, Karen E.; Digilio, Maria Cristina; Dallapiccola, Bruno; Goodship, Judith A.; Mill, Pleasantine; Lapunzina, Pablo; Ruiz-Perez, Victor L.

    2015-01-01

    Most patients with Ellis-van Creveld syndrome (EvC) are identified with pathogenic changes in EVC or EVC2, however further genetic heterogeneity has been suggested. In this report we describe pathogenic splicing variants in WDR35, encoding retrograde intraflagellar transport protein 121 (IFT121), in three families with a clinical diagnosis of EvC but having a distinctive phenotype. To understand why WDR35 variants result in EvC, we analysed EVC, EVC2 and Smoothened (SMO) in IFT-A deficient cells. We found that the three proteins failed to localize to Wdr35−/− cilia, but not to the cilium of the IFT retrograde motor mutant Dync2h1−/−, indicating that IFT121 is specifically required for their entry into the ciliary compartment. Furthermore expression of Wdr35 disease cDNAs in Wdr35−/− fibroblasts revealed that the newly identified variants lead to Hedgehog signalling defects resembling those of Evc−/− and Evc2−/− mutants. Together our data indicate that splicing variants in WDR35, and possibly in other IFT-A components, underlie a number of EvC cases by disrupting targeting of both the EvC complex and SMO to cilia. PMID:25908617

  6. Spontaneous Electrical Activity of Dionaea muscipula.

    PubMed

    Balotin, N M; Dipalma, J R

    1962-12-21

    Instillation of four to six drops of 3-percent saline in the leaf trap of Dionaea muscipula Ellis causes the appearance of a series of spontaneous action potentials. Mechanical stimulation of the sensitive leaf hair elicits only a single response. Immersion in 3-percent, but not in 1-percent, saline effects a loss of weight in Dionaea but not in the leaves of gardenia or geranium.

  7. Genipin as a novel chemical activator of EBV lytic cycle.

    PubMed

    Son, Myoungki; Lee, Minjung; Ryu, Eunhyun; Moon, Aree; Jeong, Choon-Sik; Jung, Yong Woo; Park, Gyu Hwan; Sung, Gi-Ho; Cho, Hyosun; Kang, Hyojeung

    2015-02-01

    Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that causes acute infection and establishes life-long latency. EBV causes several human cancers, including Burkitt's lymphoma, nasopharyngeal and gastric carcinoma. Antiviral agents can be categorized as virucides, antiviral chemotherapeutic agents, and immunomodulators. Most antiviral agents affect actively replicating viruses, but not their latent forms. Novel antiviral agents must be active on both the replicating and the latent forms of the virus. Gardenia jasminoides is an evergreen flowering plant belonging to the Rubiaceae family and is most commonly found growing wild in Vietnam, Southern China, Taiwan, Japan, Myanmar, and India. Genipin is an aglycone derived from an iridoid glycoside called geniposide, which is present in large quantities in the fruit of G. jasminoides. In this study, genipin was evaluated for its role as an antitumor and antiviral agent that produces inhibitory effects against EBV and EBV associated gastric carcinoma (EBVaGC). In SNU719 cells, one of EBVaGCs, genipin caused significant cytotoxicity (70 μM), induced methylation on EBV C promoter and tumor suppressor gene BCL7A, arrested cell-cycle progress (S phases), upregulated EBV latent/lytic genes in a dose-dependent manner, stimulated EBV progeny production, activated EBV F promoter for EBV lytic activation, and suppressed EBV infection. These results indicated that genipin could be a promising candidate for antiviral and antitumor agents against EBV and EBVaGC. PMID:25626372

  8. Genipin as a novel chemical activator of EBV lytic cycle.

    PubMed

    Son, Myoungki; Lee, Minjung; Ryu, Eunhyun; Moon, Aree; Jeong, Choon-Sik; Jung, Yong Woo; Park, Gyu Hwan; Sung, Gi-Ho; Cho, Hyosun; Kang, Hyojeung

    2015-02-01

    Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that causes acute infection and establishes life-long latency. EBV causes several human cancers, including Burkitt's lymphoma, nasopharyngeal and gastric carcinoma. Antiviral agents can be categorized as virucides, antiviral chemotherapeutic agents, and immunomodulators. Most antiviral agents affect actively replicating viruses, but not their latent forms. Novel antiviral agents must be active on both the replicating and the latent forms of the virus. Gardenia jasminoides is an evergreen flowering plant belonging to the Rubiaceae family and is most commonly found growing wild in Vietnam, Southern China, Taiwan, Japan, Myanmar, and India. Genipin is an aglycone derived from an iridoid glycoside called geniposide, which is present in large quantities in the fruit of G. jasminoides. In this study, genipin was evaluated for its role as an antitumor and antiviral agent that produces inhibitory effects against EBV and EBV associated gastric carcinoma (EBVaGC). In SNU719 cells, one of EBVaGCs, genipin caused significant cytotoxicity (70 μM), induced methylation on EBV C promoter and tumor suppressor gene BCL7A, arrested cell-cycle progress (S phases), upregulated EBV latent/lytic genes in a dose-dependent manner, stimulated EBV progeny production, activated EBV F promoter for EBV lytic activation, and suppressed EBV infection. These results indicated that genipin could be a promising candidate for antiviral and antitumor agents against EBV and EBVaGC.

  9. Elucidation of Danzhixiaoyao Wan and Its Constituent Herbs on Antioxidant Activity and Inhibition of Nitric Oxide Production

    PubMed Central

    Banbury, Linda K.; Leach, David N.

    2007-01-01

    Danzhixiaoyao Wan (DW) is a common 10 herbs formulation in China for regulating several clinical conditions affecting women. This research tried to explain one of DW's functions, purging heat, using in vitro pharmacological analyses. The whole formulation and each single herb of DW were compared based on antioxidant activity with the oxygen radical absorbance capacity (ORAC) assay, and for their inhibitory effect (IE) on nitric oxide (NO) production by lipopolysaccharide (LPS)-activated RAW 264.7 macrophages with the Griess assay. The results showed that DW as a whole formulation had both antioxidant activity and an IE on NO production, while the individual herb component of DW varied in their ORAC values and inhibition of NO production. The ORAC value of the whole DW was 450 μmol TE g−1. The order of antioxidant (ORAC) activity of the single herbs was: Mentha haplocalyx (1352 μmol TE g−1) > Glycyrrhiza uralensis (1184 μmol TE g−1) > Gardenia jasminoides (1129 μmol TE g−1) > Paeonia suffruticosa (465 μmol TE g−1), with the contributions being additive rather than synergistic. The production of nitrite by stimulated RAW 264.7 murine macrophages (unstimulated: 0.5 ± 0.1 μM versus LPS: 38.9 ± 2.3 μM) was significantly inhibited (P < 0.05) by M. haplocalyx, G. jasminoides, Bupleurum chinense and Paeonia lactiflora. DW as a whole had an IE on NO production, but this was not significant. The single herb M. haplocalyx had the highest ORAC value and the highest IE on NO production, followed by G. jasminoides. Both of these herbs have the ‘purging heat’ property in the theory of traditional Chinese medicine and this property of the samples may be correlated with the antioxidant activity and IE on NO production. PMID:18227909

  10. Ecology and behavior of Pezothrips kellyanus (Thysanoptera: Thripidae) on citrus.

    PubMed

    Vassiliou, V A

    2010-02-01

    The most common thrips species found in Cyprus citrus orchards between 2003 and 2008 were Pezothrips kellyanus (Bagnall) (Thysanoptera: Thripidae), Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), and Thrips tabaci (Lindeman) (Thysanoptera: Thripidae). Only Pezothrips kellyanus, Kelly's citrus thrips (KCT) causes feeding damage on citrus fruits in Cyprus. KCT adults prefer to concentrate mostly in the northern and eastern sides of both lemon and grapefruit canopies. The attractiveness of white, sky blue, marine blue, and yellow color to KCT was evaluated. White was found to be the most attractive color to adults of KCT, F. occidentalis, and T. tabaci. A range of incidental and breeding host plants grown within and outside citrus orchards in Cyprus were identified. KCT adults were found on flowers of all citrus varieties, and various other flowering plants including Malva nicaeensis, Malva silvestris, Sinapis alba, Oxalis pes-caprae, Calendula arvensis, Urospermum picroides, Jasminum officinale, Gardenia jasminoides, Jasminum sambac, Prunus dulcis, Mangifera indica, Persea americana, and Eriobotrya japonica. KCT larvae were found only on lemon, grapefruit, Jasmine spp., and Gardenia flowers. PMID:20214367

  11. Charge induced closing of Dionaea muscipula Ellis trap.

    PubMed

    Volkov, Alexander G; Adesina, Tejumade; Jovanov, Emil

    2008-11-01

    In terms of bioelectrochemistry, Venus flytrap responses can be considered in three stages: stimulus perception, electrical signal transmission, and induction of mechanical and biochemical responses. When an insect touches the trigger hairs, these mechanosensors generate receptor potentials, which induce solitary waves activating the motor cells. We found that the electrical charge injected between a midrib and a lobe closes the Venus flytrap leaf by activating motor cells without mechanical stimulation of trigger hairs. The mean electrical charge required for the closure of the Venus flytrap leaf is 13.6 muC. To close the trap, electrical charge can be submitted as a single charge or applied cumulatively by small portions during a short period of time. Ion channel blocker such as Zn(2+) as well as an uncoupler CCCP, dramatically decreases the speed of the trap closing a few hours after treatment of the soil. This effect is reversible. After soil washing by distilled water, the closing time of Venus flytrap treated by CCCP or ZnCl(2) decreases back from 2-5 s to 0.3 s, but higher electrical charge is needed for trap closure. The mechanism behind closing the upper leaf of Venus flytrap is discussed.

  12. Simultaneous Determination of Catalpol, Aucubin, and Geniposidic Acid in Different Developmental Stages of Rehmannia glutinosa Leaves by High Performance Liquid Chromatography

    PubMed Central

    Wang, Yanjie; Liao, Dengqun; Qin, Minjian

    2016-01-01

    Although R. glutinosa roots are currently the only organ source in clinics, its leaves are a potential supplement for the roots especially in extraction of some important bioactive compounds. Our early work found that the contents of catalpol and total iridoid glycosides varied among different developmental stages of R. glutinosa leaves. Aucubin and geniposidic acid, the abundant major bioactive compounds in Eucommia ulmoides and Gardenia jasminoides, respectively, were found present in R. glutinosa roots, however, and have not been analyzed in its leaves. In this paper, we aimed to determine contents of these three iridoid glycosides in different developmental stages of R. glutinosa leaves using the optimized HPLC-UV conditions. Our results showed that aucubin and GPA in R. glutinosa leaves were much lower than catalpol and showed the increasing trend with the leaf development, which was different from catalpol. This work provided the important information for future exploitation of R. glutinosa leaves as a potential supplement for its roots in extraction of some important bioactive compounds and studying the relationship of aucubin and catalpol metabolism. PMID:27429834

  13. A sensitive liquid chromatographic-mass spectrometric method for simultaneous quantification of six iridoid glycosides from Zhi-zi-chi Decoction in rat plasma and its application to a pharmacokinetic study.

    PubMed

    Qu, Kankan; Dai, Jinna; Zhao, Longshan; Lu, Yanan; Li, Bin; Zhao, Xu; Hou, Pengyi; Zhang, Yuanting; Bi, Kaishun; Chen, Xiaohui

    2013-05-01

    A sensitive liquid chromatographic-mass spectrometric (LC-MS) method was developed and validated for simultaneous determination of geniposide, geniposidic acid, scandoside methyl ester, gardenoside, deacetyl asperulosidic acid methyl ester and genipin-1-β-gentiobioside after oral administration of Zhi-zi-chi Decoction in rat plasma. The six iridoid glycosides were extracted from plasma samples by protein precipitation, and then separated on an Apollo C18 column (250 mm × 4.6mm, 5 μm) through the application of a gradient elution. The analytes were monitored in positive electrospray ionization by selected ion monitoring mode (SIM). The lower limits of quantitation (LLOQ) of the six analytes were all lower than 6 ng/mL. The accuracy (relative error, RE%) was between -7.0% and 9.9%, while the intra- and inter-day precisions (relative standard deviation, RSD%) were less than 6.3% and 9.8% for the six analytes, respectively. The developed method was successfully applied to a comparative pharmacokinetic study of the six iridoids in rat plasma after oral administration of Zhi-zi-chi Decoction and Gardenia jasminoides extract.

  14. Anti-Adipogenic Effects of Ethanol Extracts Prepared from Selected Medicinal Herbs in 3T3-L1 Cells

    PubMed Central

    Park, Min-Jun; Song, Ji-Hye; Shon, Myung-Soo; Kim, Hae Ok; Kwon, O Jun; Roh, Seong-Soo; Kim, Choon Young; Kim, Gyo-Nam

    2016-01-01

    Obesity is a major risk factor for various metabolic diseases such as cardiovascular disease, hypertension, and type 2 diabetes mellitus. In this study, we prepared ethanol extracts from Agastache rugosa (ARE), Chrysanthemum zawadskii (CZE), Mentha arvensis (MAE), Perilla frutescens (PFE), Leonurus sibiricus (LSE), Gardenia jasminoides (GJE), and Lycopus coreanus (LCE). The anti-oxidant and anti-adipogenic effects were evaluated. The IC50 values for ascorbic acid and LCE against 2,2-diphenyl-1-picrylhydrazyl radicals were 246.2 μg/mL and 166.2 μg/mL, respectively, followed by ARE (186.6 μg/mL), CZE (198.6 μg/mL), MAE (337.1 μg/mL), PFE (415.3 μg/mL), LSE (548.2 μg/mL), and GJE (626.3 μg/mL). In non-toxic concentration ranges, CZE had a strong inhibitory effect against 3T3-L1 adipogenes (84.5%) than those of the other extracts. Furthermore, the anti-adipogenic effect of CZE is largely limited in the early stage of adipogenesis, and we revealed that the inhibitory role of CZE in adipogenesis is required for the activation of Wnt signaling. Our results provide scientific evidence that the anti-adipogenic effect of CZE can be applied as an ingredient for the development of functional foods and nutri-cosmetics for obesity prevention. PMID:27752499

  15. [Selection and purification potential evaluation of woody plant in vertical flow constructed wetlands in the subtropical area].

    PubMed

    Chen, Yong-Hua; Wu, Xiao-Fu; Hao, Jun; Chen, Ming-Li; Zhu, Guang-Yu

    2014-02-01

    In order to solve the problem that wetland herbaceous plants tend to die during winter in subtropics areas, selection and purification potential evaluation experiments were carried out by introducing into the constructed wetlands 16 species of woody wetland plants. Cluster analysis was performed by including the morphological characteristics, physiological characteristics, as well as nitrogen and phosphorus accumulation of the woody wetland plants. The results indicated that there were significant differences among the tested woody plants in their survival rate, height increase, root length increase and vigor, Chlorophyll content, Superoxide dismutase, Malonaldehyde, Proline, Peroxidase, biomass, average concentration and accumulation of nitrogen and phosphorus. Based on the established evaluation system, the tested plants were clustered into 3 groups. The plants in the 1st group possessing high purification potentials are Nerium oleander and Hibiscus syriacus. Those in the 2nd group possessing moderate purification potentials are Trachycarpus fortune, Llex latifolia Thunb., Gardenia jasminoides, Serissa foetida and Ilex crenatacv Convexa. And those in the 3rd group with low purification potentials are Jasminum udiflorum, Hedera helix, Ligustrum vicaryi, Ligustrum lucidum, Buxus sempervives, Murraya paniculata, Osmanthus fragrans, Mahoniafortune and Photinia serrulata. PMID:24812951

  16. Norartocarpetin from a folk medicine Artocarpus communis plays a melanogenesis inhibitor without cytotoxicity in B16F10 cell and skin irritation in mice

    PubMed Central

    2013-01-01

    Background Many natural products used in preventive medicine have also been developed as cosmeceutical ingredients in skin care products, such as Scutellaria baicalensis and Gardenia jasminoides. Norartocarpetin is one of the antioxidant and antityrosinase activity compound in Artocarpus communis; however, the cytotoxicity, skin irritation and antimelanogenesis mechanisms of norartocarpetin have not been investigated yet. Methods In the present study, cell viability in vitro and skin irritation in vivo are used to determine the safety of norartocarpetin. The melanogenesis inhibition of norartocarpetin was determined by cellular melanin content and tyrosinase in B16F10 melanoma cell. Moreover, we examined the related-melanogenesis protein by western blot analysis for elucidating the antimelanogenesis mechanism of norartocarpin. Results The result of the present study demonstrated that norartocarpetin not only present non-cytotoxic in B16F10 and human fibroblast cells but also non-skin irritation in mice. Moreover, our result also first found that norartocarpetin downregulated phospho-cAMP response element-binding (phospho-CREB) and microphthalmia-associated transcription factor (MITF) expression, which in turn decreased both synthesis of tyrosinases (TRP-1 and TRP-2) and cellular melanin content. This process is dependent on norartocarpetin phosphorylation by mitogen-activated protein kinases such as phospho-JNK and phospho-p38, and it results in decreased melanogenesis. Conclusion The present study suggests that norartocarpetin could be used as a whitening agent in medicine and/or cosmetic industry and need further clinical study. PMID:24325567

  17. "Their Experience Is the Immigrant Experience": Ellis Island, Documentary Film, and Rhetorically Reversible Whiteness

    ERIC Educational Resources Information Center

    Irwin, Meryl J.

    2013-01-01

    Political advocates on the ideological right have long taken seriously what their counterparts on the left have not: white racialized affect. As left activists and scholars have alternately lamented and raged over the steady creep of the "middle" to the "right," they have documented in detail the outcomes of whites' refusal to engage in "genuine"…

  18. The nature of the stimuli causing digestive juice secretion in Dionaea muscipula Ellis (venus's flytrap).

    PubMed

    Robins, R J

    1976-01-01

    An investigation into the stimuli of the secretory system in the carnivorous plant Dionaea muscipula is presented. Secretion of fluid and protein are both stimulated by various nitrogenous small molecules. These secretions are studied as a function of time. A new method is described for the collection of secreted juice. Significant differences are found between the quantities of fluid and protein produced in response to different stimuli. The results are discussed in comparison to the mammalian gastro-intestinal secretory systems.

  19. Polarity in mechanoreceptor cells of trigger hairs of Dionaea muscipula Ellis.

    PubMed

    Buchen, B; Hensel, D; Sievers, A

    1983-08-01

    Both the apical and the basal cell poles of the sensory cells in trigger hairs of Dionaea muscipula are structured identically. A complex of concentrically arranged endoplasmic reticulum cisternae occupies each of the poles. One to four vacuoles are enclosed within the central cisterna and contain polyphenols (deposits of "tannin"). Structural polarity, whether symmetric or asymmetric, as well as the occurrence of abundant endoplasmic reticulum and numerous mitochondria are characteristics of the perception cells of most animals and plants.

  20. Acid phosphatase localization in the digestive glands of Dionaea muscipula Ellis flytraps.

    PubMed

    Henry, Y; Steer, M W

    1985-04-01

    The intracellular localization of acid phosphatases in stimulated digestive glands of Dionaea flytraps has been studied to provide evidence for the route taken by this enzyme during secretion. Previous studies have either included or excluded a role for the dictyosomes in this pathway. Both p-nitrophenyl phosphate and beta-glycerophosphate were used as substrates, and both gave similar localization patterns. Unstimulated glands contained little phosphatase activity in the endomembrane system, whereas 24 and 48 hr after stimulation, heavy deposits of lead were located in the endoplasmic reticulum cisternae, including the nuclear envelope, the dictyosome cisternae, and secretory vesicles. Since dictyosome activation, as judged by the presence of secretory vesicles in the cytoplasm, also coincides with gland stimulation, we conclude that secretion of the hydrolase enzymes occurs via this route and not, as suggested elsewhere, via direct endoplasmic reticulum to plasma membrane connections.

  1. Immigration: From the Founding of Virginia to the Closing of Ellis Island. An Eyewitness History.

    ERIC Educational Resources Information Center

    Wepman, Dennis

    It can be said that U.S. immigration started in 1607, when the first settlers joined the original colonists on the shores of the United States. Since then, people of every nation, ethnicity, and class have come to the United States, looking for freedom, prosperity, and stability. While originally immigrants flowed freely into the United States,…

  2. Comments of Rod Ellis's "The Structural Syllabus and Second Language Acquisition."

    ERIC Educational Resources Information Center

    Robinson, Peter; Ellis, Rod

    1994-01-01

    An argument for the role of a structural syllabus as a means to promote "gradual mastery" of implicit second-language knowledge is refuted. The author responds with further discussion of the relationship between explicit and implicit knowledge. (Contains 13 references.) (LB)

  3. Immigration beyond Ellis Island: Suggestions for Teaching about Immigration in the Now

    ERIC Educational Resources Information Center

    Hossain, Kazi I.

    2014-01-01

    America's history books abound with stories of immigrants who contributed to the development of this country. In terms of social studies curriculum, all states require schools to teach about immigration. However, the question is how to teach this topic in a manner that will give students--elementary through high school--a better understanding of…

  4. A Resource Guide for Celebrating the Statue of Liberty and Ellis Island, 1986. Bulletin 1783.

    ERIC Educational Resources Information Center

    Kerr, Myrtle; Cookston, James S.

    Compiled to help celebrate the 100th anniversary of the Statue of Liberty, this selection of books, periodicals, audiovisuals, and other educational resources was intended to help stimulate discussion, promote projects, and create awareness of the Statue of Liberty Centennial. Listed are 24 books, 18 magazine articles, and 3 films about the Statue…

  5. Choanoflagellate lorica construction and assembly: the nudiform condition. II. Acanthoeca spectabilis Ellis.

    PubMed

    Leadbeater, Barry S C; Henouil, Magali; Berovic, Nikolas

    2008-07-01

    Acanthoeca spectabilis is one of the most common loricate choanoflagellates found in marine biofilms everywhere. However, it is special for two reasons; firstly, it is probably the most distinctive member of the small nudiform clade of loricate choanoflagellates. Secondly, the lorica chamber of Acanthoeca comprises a closely wound left-handed coil of costae that is unique amongst choanoflagellates. Mathematical analysis of the lorica chamber shows that the helical costae undergo two turns. This species, more than any, demonstrates that the helical coiling of costae can only be achieved by a rotational movement generated by the cell during lorica assembly. Comparison of the lorica morphology of Acanthoeca with that of the closely related genus Polyoeca indicates that the helical costae of Acanthoeca are probably homologous with the outer longitudinal costae of Polyoeca. This is unusual because helical costae are usually the innermost layer of costae. However, since there is no 'true' inner layer in the chamber of Acanthoeca the outer layer of costae are adjacent to the cell surface and therefore available for coiling. In contrast to tectiform choanoflagellates, which number more than one hundred species and inhabit a wide variety of microniches, the six known extant nudiform species must either represent a minor evolutionary development or be the remnants of a previously more extensive radiation.

  6. Gravity Currents: In the Environment and the Laboratory, Ellis Horwood Series in Environmental Sciences

    NASA Astrophysics Data System (ADS)

    Csanady, G. T.

    There are books and courses on experimental physics, thorough and rigorous, which contain only simple formulae and use only elementary algebra. The subject is explained in lucid and precise language, with the help of many sketches, photos, and diagrams. The book under review belongs to this genre and is in fact one of its most successful members.About half of the book is devoted to the description of a variety of natural phenomena in which gravity currents play an important role. The class of gravity currents is taken to be quite broad, to include almost any kind of quasi-horizontal motion caused by density differences. The range of natural phenomena surveyed is correspondingly great, from tidal bores through thunderstorms to the eruption of Mount St. Helens. The atmospheric examples are especially thorough and interesting. The broad panorama certainly brings home to the reader the many ways in which fluids (including fluidized rock) can move under the influence of gravity. However, not all of the examples show typical gravity behavior, nor are they all treated with equal thoroughness. The Gulf Stream is mentioned, and the variability of its position is shown in an illustration, but this is not connected with anything typical of gravity currents, nor is the important interplay between Earth rotation and such large-scale “gravity currents” (if one insists on calling them that) at all adequately explained.

  7. A Comparison of Rational Emotive Therapy and Tibetan Buddhism: Albert Ellis and the Dalai Lama

    ERIC Educational Resources Information Center

    Holt, Susan A; Austad, Carol Shaw

    2013-01-01

    This article explores conceptual and methodological similarities between Rational Emotive Behavior Therapy (REBT) and Tibetan Buddhism (TB). The authors examine some of the values and concepts they share. They compare the two systems on a number of issues: philosophical underpinnings, concepts of what causes human psychopathology, techniques to…

  8. From Ellis Island to LAX: immigrant prospects in the American city.

    PubMed

    Waldinger, R

    1996-01-01

    "This article seeks to bring the ¿urban' back into immigration research. Each immigrant receiving area has its own particular group of newcomers, and the economic and political structures of the immigrant receiving areas are also distinctive. Those structures are not all determining, as immigrant trajectories are shaped by the interaction between distinctive urban institutions and the specific characteristics of the relevant ethnic groups. But in the last analysis, the urban context makes a difference, as this study shows by examining the leading [U.S.] immigrant destinations--New York and Los Angeles."

  9. On the mechanism underlying photosynthetic limitation upon trigger hair irritation in the carnivorous plant Venus flytrap (Dionaea muscipula Ellis).

    PubMed

    Pavlovic, Andrej; Slováková, L'udmila; Pandolfi, Camilla; Mancuso, Stefano

    2011-03-01

    Mechanical stimulation of trigger hairs on the adaxial surface of the trap of Dionaea muscipula leads to the generation of action potentials and to rapid leaf movement. After rapid closure secures the prey, the struggle against the trigger hairs results in generation of further action potentials which inhibit photosynthesis. A detailed analysis of chlorophyll a fluorescence kinetics and gas exchange measurements in response to generation of action potentials in irritated D. muscipula traps was used to determine the 'site effect' of the electrical signal-induced inhibition of photosynthesis. Irritation of trigger hairs and subsequent generation of action potentials resulted in a decrease in the effective photochemical quantum yield of photosystem II (Φ(PSII)) and the rate of net photosynthesis (A(N)). During the first seconds of irritation, increased excitation pressure in photosystem II (PSII) was the major contributor to the decreased Φ(PSII). Within ∼1 min, non-photochemical quenching (NPQ) released the excitation pressure at PSII. Measurements of the fast chlorophyll a fluorescence transient (O-J-I-P) revealed a direct impact of action potentials on the charge separation-recombination reactions in PSII, although the effect seems to be small rather than substantial. All the data presented here indicate that the main primary target of the electrical signal-induced inhibition of photosynthesis is the dark reaction, whereas the inhibition of electron transport is only a consequence of reduced carboxylation efficiency. In addition, the study also provides valuable data confirming the hypothesis that chlorophyll a fluorescence is under electrochemical control.

  10. Is Angel Island the Ellis Island of the West? Teaching Multiple Perspective-Taking in American Immigration History

    ERIC Educational Resources Information Center

    Ciardiello, A. Vincent

    2012-01-01

    Education in the twenty-first century requires that all students learn the social studies skills that help them understand the complex issues related to civic ideals and practices. One of these skills is developing multiple perspective-taking. Many educators believe that this critical thinking ability is not only for upper level students but also…

  11. Production and carbonate dynamics of Halimeda incrassata (Ellis) Lamouroux altered by Thalassia testudinum Banks and Soland ex König

    NASA Astrophysics Data System (ADS)

    Barry, S.; Frazer, T.; Jacoby, C.

    2013-05-01

    Ocean acidification poses a serious threat to a broad suite of calcifying organisms. Scleractinian corals and calcareous algae that occupy shallow, tropical waters are vulnerable to global changes in ocean chemistry because they already are subject to stressful and variable carbon dynamics at the local scale. For example, net heterotrophy increases carbon dioxide concentrations, and pH varies with diurnal fluctuations in photosynthesis and respiration. Few researchers, however, have investigated the possibility that carbon dioxide consumption during photosynthesis by non-calcifying photoautotrophs, such as seagrasses, can ameliorate deleterious effects of ocean acidification on sympatric calcareous algae. Naturally occurring variations in the density of seagrasses and associated calcareous algae provide an ecologically relevant test of the hypothesis that diel fluctuations in water chemistry driven by cycles of photosynthesis and respiration within seagrass beds create microenvironments that enhance macroalgal calcification. In Grape Tree Bay off Little Cayman Island BWI, we quantified net production and characterized calcification for thalli of the calcareous green alga Halimeda incrassata growing within beds of Thalassia testudinum with varying shoot densities. Results indicated that individual H. incrassata thalli were ~6% more calcified in dense seagrass beds. On an areal basis, however, far more calcium carbonate was produced by H. incrassata in areas where seagrasses were less dense due to higher rates of production. In addition, diel pH regimes in vegetated and unvegetated areas within the lagoon were not significantly different, suggesting water exchange and mixing throughout the lagoon. These results suggest that, especially in well-mixed lagoons, carbonate production by calcareous algae may be more related to biotic interactions between seagrasses and calcareous algae than to seagrass-mediated changes in local water chemistry.

  12. Society News: RAS Awards 2011; Prof. Richard Ellis; Prof. Eberhard Grün; Prof. Gilles Chabrier; Prof. Roger Searle;

    NASA Astrophysics Data System (ADS)

    2011-02-01

    Each year the RAS recognizes outstanding achievement in astronomy and geophysics by the award of medals and prizes. Candidates are nominated by Fellows and the awards made by a committee of Fellows, ensuring that these scientists have earned the respect and admiration of their peers in the research community.

  13. 75 FR 61706 - Reorganization of Foreign-Trade Zone 113 Under Alternative Site Framework; Ellis County, TX

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-06

    ... Board adopted the alternative site framework (ASF) in December 2008 (74 FR 1170, 01/12/09; correction 74 FR 3987, 01/22/09) as an option for the establishment or reorganization of general-purpose zones... inviting public comment was given in the Federal Register (75 FR 3705, 01/22/10; 75 FR 17125, 04/05/10)...

  14. 75 FR 17125 - Foreign-Trade Zone 113-Ellis County, Texas, Application for Reorganization under Alternative Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-05

    ... (75 FR 3705, 1/22/2010), is being extended to April 29, 2010 to allow interested parties additional....gov or (202) 482-2350. Dated: March 25, 2010. Andrew McGilvray, Executive Secretary. BILLING CODE...

  15. The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.

    PubMed

    Clayton, Aled; Lawson, Charlotte; Gardiner, Chris; Harrison, Paul; Carter, David

    2016-01-01

    The UK Extracellular Vesicles (UKEV) Forum meetings were born of the realization that there were a number of UK laboratories studying extracellular vesicle biology and using similar techniques but without a regular national meeting dedicated to EVs at which to share their findings. This was compounded by the fact that many of these labs were working in different fields and thus networking and sharing of ideas and best practice was sometimes difficult. The first workshop was organized in 2013 by Dr Charlotte Lawson, under the auspices of the Society for Endocrinology, led to the founding of the UKEV Forum and the organization of a British Heart Foundation sponsored 1-day conference held in London in December 2014. Although growing in size every year, the central aims of these workshops have remained the same: to provide a forum for discussion and exchange of ideas, to allow young scientists to present their data in the form of short talks and poster presentations and to discuss their work with more established scientists in the field. Here we include the presented abstracts for the 2015 1-day conference hosted by Cardiff University. This meeting was attended by approximately 130 delegates throughout the United Kingdom, but also attended by delegates from Belgium, Netherlands, France, Ireland and other nations. The day composed of plenary presentations from Prof Matthias Belting, Lund University, Sweden and Dr Guillaume van Niel, Institut Curie, Paris together with 10 short presentations from submitted abstracts. The topics covered were broad, with sessions on Mechanisms of EV production, EVs in Infection, EVs in Cancer and in Blood and Characterizing EVs in Biological fluids. This hopefully gives a reflection of the range of EV-related studies being conducted currently in the UK. There were also 33 poster presentations equally broad in subject matter. The organizers are grateful to the Life Science Research Network Wales - a Welsh government-funding scheme that part-sponsored the conference. We are also grateful to commercial sponsors, and 3 paid-presentations are included in the abstracts. The UK EV Forum is expected to become an established annual event held at different Universities across the UK and continue to attract increasing delegate numbers and abstract submissions. We look forward to the next planned conference, which will be hosted by David Carter and his colleagues at Oxford Brookes University on 13th December 2016. PMID:26928673

  16. Genipin is active via modulating monoaminergic transmission and levels of brain-derived neurotrophic factor (BDNF) in rat model of depression.

    PubMed

    Wang, Q-S; Tian, J-S; Cui, Y-L; Gao, S

    2014-09-01

    Genipin, an important bioactive component from Gardenia jasminoides Eills, was demonstrated to possess antidepressant-like effects in a previous study. However, the molecular mechanism of antidepressant-like effects on genipin was not clear. The present study aimed to investigate the possible mechanism of antidepressant-like effects on genipin with a chronic unpredictable mild stress (CUMS)-induced depression model in rats. In CUMS-induced depressive rats, bodyweight and 1% sucrose consumption decreased significantly compared with the normal control group. Furthermore, these changes could be significantly reversed by genipin application. The levels of 5-hydroxytryptamine (5-HT), norepinephrine (NE) in the hippocampus decreased and the level of 5-hydroxyindole acetic acid (5-HIAA) increased in the CUMS-induced depressive rats. However, pre-treatments with genipin significantly increased the levels of 5-HT, NE and decreased the level of 5-HIAA in the hippocampus. The concentration of cAMP in the hippocampus was increased by genipin compared to the CUMS-exposed model group. The mRNA expressions of 5-hydroxytryptamine 1A receptor (5-HT1AR), cAMP response element binding protein (CREB) and brain-derived neurotrophic factor (BDNF) in rats were decreased exposed to CUMS, which were reversed by genipin-treated rats exposed to CUMS. Compared to the CUMS-exposed model group, the mRNA expression of 5-hydroxytryptamine 2A receptor (5-HT(2A)R) was decreased significantly by genipin-treated rats. The mRNA and protein expression of CREB, BDNF were increased in genipin-treated rats compared to the CUMS-exposed model group. Moreover, the levels of corticosterone in serum were decreased by genipin-treated compared to the CUMS-exposed model group. These results suggest that the possible mechanism of antidepressant-like effects on genipin, at least in one part, resulted from monoaminergic neurotransmitter system and the potential dysfunctional regulation of the post-receptor signaling

  17. 78 FR 27415 - Kansas; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-10

    ... major disaster: Barber, Barton, Dickinson, Ellis, Franklin, Harper, Harvey, Hodgeman, Kingman, Marion... the Public Assistance program. Barton, Dickinson, Ellis, Franklin, Harper, Harvey, Hodgeman,...

  18. Psychotherapy as Stochastic Process: Fitting a Markov Chain Model to Interviews of Ellis and Rogers. University of Minnesota Office of Student Affairs Research Bulletin, Vol. 15, No. 18.

    ERIC Educational Resources Information Center

    Lichtenberg, James W.; Hummel, Thomas J.

    This investigation tested the hypothesis that the probabilistic structure underlying psychotherapy interviews is Markovian. The "goodness of fit" of a first-order Markov chain model to actual therapy interviews was assessed using a x squared test of homogeneity, and by generating by Monte Carlo methods empirical sampling distributions of selected…

  19. Abundance of Cysteine Endopeptidase Dionain in Digestive Fluid of Venus Flytrap (Dionaea muscipula Ellis) Is Regulated by Different Stimuli from Prey through Jasmonates

    PubMed Central

    Libiaková, Michaela; Floková, Kristýna; Novák, Ondřej; Slováková, L'udmila; Pavlovič, Andrej

    2014-01-01

    The trap of the carnivorous plant Venus flytrap (Dionaea muscipula) catches prey by very rapid closure of its modified leaves. After the rapid closure secures the prey, repeated mechanical stimulation of trigger hairs by struggling prey and the generation of action potentials (APs) result in secretion of digestive fluid. Once the prey's movement stops, the secretion is maintained by chemical stimuli released from digested prey. We investigated the effect of mechanical and chemical stimulation (NH4Cl, KH2PO4, further N(Cl) and P(K) stimulation) on enzyme activities in digestive fluid. Activities of β-D-glucosidases and N-acetyl-β-D-glucosaminidases were not detected. Acid phosphatase activity was higher in N(Cl) stimulated traps while proteolytic activity was higher in both chemically induced traps in comparison to mechanical stimulation. This is in accordance with higher abundance of recently described enzyme cysteine endopeptidase dionain in digestive fluid of chemically induced traps. Mechanical stimulation induced high levels of cis-12-oxophytodienoic acid (cis-OPDA) but jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) accumulated to higher level after chemical stimulation. The concentration of indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA) did not change significantly. The external application of JA bypassed the mechanical and chemical stimulation and induced a high abundance of dionain and proteolytic activity in digestive fluid. These results document the role of jasmonates in regulation of proteolytic activity in response to different stimuli from captured prey. The double trigger mechanism in protein digestion is proposed. PMID:25153528

  20. The Study of Two Psychotherapy Approaches (Rogers Self Theory and Ellis Rational Theory) in Improvement of Bowen Self-differentiation and Intimacy

    PubMed Central

    Yousefi, Naser; Kiani, Mohammad Ali

    2014-01-01

    Objective: To compare the effectiveness of rational, behavioral and emotive therapy (REBT) and person-centered therapy (PCT) on self-differentiation and intimacy among divorce clients. Methods: In quasi-experimental study, 42 divorce clients (both males and females) who presented to the Counsling Center of Sanandaj, Iran were sampled. They were categorized into three groups of PCT, REBT, and control group (each group contained 14 subjects). The recovery indices (dependent variables) employed were the subject of self-differentiation and intimacy, which were measured twice before and after intervention of Differentiation of Self Inventory-2 (DSI-2) and intimacy. The therapy involved 8 one-hour sessions. It was held twice a week and therapeutic effects were traced after 8 months. Results: The results showed that REBT and PCT were effective on self-differentiation scale and intimacy. Also they were influential in recovery self-differentiation scale and intimacy follow up stage. Conclusion: REBT and PCT were effective on self-differentiation and its subscales (Emotional reactivity, “I” position, Emotional cut off and Fusion with other) and general intimacy. Declaration of interest: None. PMID:24995028

  1. Modeling of ground-water flow in subsurface Austin Chalk and Taylor marl in Ellis County, Texas, near the superconducting super collider site

    SciTech Connect

    Mace, R.E. . Bureau of Economic Geology)

    1993-02-01

    Numerical models are useful tools for developing an understanding of ground-water flow in sparsely characterized low-permeability aquifers. Finite-difference, cross-sectional models of Cretaceous chalk and marl formations near the Superconducting Super Collider (SSC) were constructed using MODFLOW to evaluate ground-water circulation paths and travel times. Weathered and fractured zones with enhanced permeability were included to assess the effect these features had on flow paths and times. Pump tests, slug tests, packer tests, core tests, and estimates were used to define hydraulic properties for model input. The model was calibrated with water-level data from monitor wells and from wire-line piezometers near a test shaft excavated by the SSC project. A ratio of vertical-to-horizontal permeability of 0.0085 was estimated through model calibration. A chalk-to-marl permeability ratio of 18 was needed to reproduce artesian head in a well completed in chalk beneath marl. Hydraulic head distributions and ground-water flow paths reflected local, intermediate, and regional flow systems with recharge beneath upland surface-water divides and discharge in valleys. Most of the flow (99%) occurred in the weathered zone, with average residence times of 5 to 10 years. Residence time in unweathered chalk bedrock was substantially longer, at an average of 1.7 Ma. As expected, the model demonstrated that deep and rapid ground-water circulation might occur in fracture zones. Particle paths calculated using MODPATH showed that ground-water travel times from recharge areas to the SSC subsurface facilities might be 20 to 60 years where flow is through fracture zones.

  2. The Roles of Attention and (Un)awareness in SLA: Conceptual Replication of N. C. Ellis & Sagarra (2010a) and Leung & Williams (2012)

    ERIC Educational Resources Information Center

    Leow, Ronald P.

    2015-01-01

    There is no doubt that attention and (un)awareness in second/foreign language (L2 learning) are two constructs that have permeated, explicitly or implicitly, second language acquisition (SLA) studies since their inception. Indeed, we have witnessed several empirical studies attempting to probe more deeply into the roles of these two constructs in…

  3. Abundance of cysteine endopeptidase dionain in digestive fluid of Venus flytrap (Dionaea muscipula Ellis) is regulated by different stimuli from prey through jasmonates.

    PubMed

    Libiaková, Michaela; Floková, Kristýna; Novák, Ondřej; Slováková, L'udmila; Pavlovič, Andrej

    2014-01-01

    The trap of the carnivorous plant Venus flytrap (Dionaea muscipula) catches prey by very rapid closure of its modified leaves. After the rapid closure secures the prey, repeated mechanical stimulation of trigger hairs by struggling prey and the generation of action potentials (APs) result in secretion of digestive fluid. Once the prey's movement stops, the secretion is maintained by chemical stimuli released from digested prey. We investigated the effect of mechanical and chemical stimulation (NH4Cl, KH2PO4, further N(Cl) and P(K) stimulation) on enzyme activities in digestive fluid. Activities of β-D-glucosidases and N-acetyl-β-D-glucosaminidases were not detected. Acid phosphatase activity was higher in N(Cl) stimulated traps while proteolytic activity was higher in both chemically induced traps in comparison to mechanical stimulation. This is in accordance with higher abundance of recently described enzyme cysteine endopeptidase dionain in digestive fluid of chemically induced traps. Mechanical stimulation induced high levels of cis-12-oxophytodienoic acid (cis-OPDA) but jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) accumulated to higher level after chemical stimulation. The concentration of indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA) did not change significantly. The external application of JA bypassed the mechanical and chemical stimulation and induced a high abundance of dionain and proteolytic activity in digestive fluid. These results document the role of jasmonates in regulation of proteolytic activity in response to different stimuli from captured prey. The double trigger mechanism in protein digestion is proposed. PMID:25153528

  4. "Our Ultimate Competition," a Speech by John Neufeld, and Readers' Responses to Neufeld's "Boys Lie" by Matthew Ellis, Jaime Miller and Liz Ackert.

    ERIC Educational Resources Information Center

    Moore, John Noell, Ed.

    2000-01-01

    Suggests the ultimate competition for writers of contemporary adolescent fiction is life--the world that children inhabit is getting stranger and stranger. Discusses parents' and adolescents' different reactions to the novel "Boys Lie," which addresses the issues of rape and sexual harassment. Presents reactions to the speech and the responses of…

  5. Abundance of cysteine endopeptidase dionain in digestive fluid of Venus flytrap (Dionaea muscipula Ellis) is regulated by different stimuli from prey through jasmonates.

    PubMed

    Libiaková, Michaela; Floková, Kristýna; Novák, Ondřej; Slováková, L'udmila; Pavlovič, Andrej

    2014-01-01

    The trap of the carnivorous plant Venus flytrap (Dionaea muscipula) catches prey by very rapid closure of its modified leaves. After the rapid closure secures the prey, repeated mechanical stimulation of trigger hairs by struggling prey and the generation of action potentials (APs) result in secretion of digestive fluid. Once the prey's movement stops, the secretion is maintained by chemical stimuli released from digested prey. We investigated the effect of mechanical and chemical stimulation (NH4Cl, KH2PO4, further N(Cl) and P(K) stimulation) on enzyme activities in digestive fluid. Activities of β-D-glucosidases and N-acetyl-β-D-glucosaminidases were not detected. Acid phosphatase activity was higher in N(Cl) stimulated traps while proteolytic activity was higher in both chemically induced traps in comparison to mechanical stimulation. This is in accordance with higher abundance of recently described enzyme cysteine endopeptidase dionain in digestive fluid of chemically induced traps. Mechanical stimulation induced high levels of cis-12-oxophytodienoic acid (cis-OPDA) but jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) accumulated to higher level after chemical stimulation. The concentration of indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA) did not change significantly. The external application of JA bypassed the mechanical and chemical stimulation and induced a high abundance of dionain and proteolytic activity in digestive fluid. These results document the role of jasmonates in regulation of proteolytic activity in response to different stimuli from captured prey. The double trigger mechanism in protein digestion is proposed.

  6. Genipin Enhances Kaposi’s Sarcoma-Associated Herpesvirus Genome Maintenance

    PubMed Central

    Son, Kuwon; Park, Gyu Hwan; Jung, Jong-Wha; Shin, Yu Su; Seo, Taegun; Hyosun Cho; Kang, Hyojeung

    2016-01-01

    Kaposi's sarcoma-associated herpesvirus (KSHV) is a Gammaherpesvirus that causes acute infection and establishes life-long latency. KSHV causes several human cancers, including Kaposi's sarcoma, an acquired immune deficiency syndrome (AIDS)-related form of non-Hodgkin lymphoma. Genipin, an aglycone derived from geniposide found in Gardenia jasminoides, is known to be an excellent natural cross-linker, strong apoptosis inducer, and antiviral agent. Although evidence suggests antiviral activity of genipin in several in vitro viral infection systems, no inhibitory effect of genipin on KSHV infection has been reported. Thus, our aim was to determine, using the iSLK-BAC16 KSHV infection system, whether genipin has inhibitory effects on KSHV infection. For this purpose, we evaluated biological effects of genipin on KSHV infection and finally determined the underlying mechanisms responsible for the bioactive effects of genipin. A cytotoxicity assay revealed that genipin caused 50% cytotoxicity at 49.5 μM in iSLK-puro (KSHV-negative) cells and at 72.5 μM in iSLK-BAC16 (KSHV-positive) cells. Caspase 3/7 activities were slightly suppressed by genipin treatment in iSLK-BAC16 cells while significantly induced in iSLK-puro cells. Production of the KSHV latency-associated nuclear antigen (LANA), but not that of the R-transactivator (RTA) protein, was significantly induced by genipin treatment at lower concentration. Consistent with the LANA upregulation, KSHV LANA transcripts, but not RTA transcripts, were expressed at a higher level. Furthermore, KSHV intracellular copy numbers were slightly increased at lower concentration of genipin, while KSHV extracellular copy numbers were significantly increased at higher concentration of genipin. Interestingly, genipin treatment at a lower concentration did induce the expression of DNA (cytosine-5)-methyltransferase 1 (DNMT1); however, a co-immunoprecipitation assay showed that the DNMT1 and LANA induced by genipin did not co

  7. What Rational-Emotive Therapy Is and Is Not

    ERIC Educational Resources Information Center

    Ellis, Albert

    1974-01-01

    Albert Ellis refutes a review of the functional characteristics of Rational-Emotive Therapy given by Dugald S. Arbuckle. Ellis concludes that Arbuckle only minimally understands RET theory or the behaviors consequent to it. (PC)

  8. The Rational-Emotive Manifesto

    ERIC Educational Resources Information Center

    Ewart, Craig K.; Thoresen, Carl E.

    1977-01-01

    In a response to Albert Ellis' feature article, the author states that most of the RET statements offered in the Ellis article are very ambiguously related to the research data cited or are not supported at all. (HMV)

  9. 76 FR 52291 - Proposed Amendment of Class E Airspace; Jacksonville, NC

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-22

    ... Procedures have been developed at Albert J Ellis Airport. This action would enhance the safety and airspace... instrument approach procedures developed at Albert J Ellis Airport, Jacksonville, NC, and for continued... at Albert J Ellis Airport, Jacksonville, NC. Lists of Subjects in 14 CFR Part 71...

  10. Growth in Emotional Intelligence. Psychotherapy with a Learning Disabled Girl

    ERIC Educational Resources Information Center

    Chantrell, Sue

    2009-01-01

    This paper describes the once-weekly psychoanalytic psychotherapy of a girl, called Ellie, aged eight at the start of her treatment. Ellie had a learning disability and displayed difficult behaviour at school and at home. In her therapy, Ellie grew in emotional intelligence, more in touch with and able to express her feelings. Her behaviour…

  11. [In vitro activity of different fungicides on the growth in Mycosphaerella fijiensis var. difformis Stover and Dickson, Cladosporium musae Morelet and Deightoniella torulosa (Syd.) Ellis, isolated parasites of the banana phyllosphere in the Ivory Coast].

    PubMed

    Koné, Daouda; Badou, Odjochoumou Jean; Bomisso, Edson Lezin; Camara, Brahima; Ake, Séverin

    2009-05-01

    In Côte-d'Ivoire, banana leaf surfaces are attacked by Mycosphaerella fijiensis var. difformis, Cladosporium musae, and Deigthonielle torulosa. Control is based on fungicides recommanded for Mycosphaerella fijiensis. Fungicides belonging to triazoles and strobilurines types were added, at different concentrations, to the PDA medium, using this PDA medium containing no fungicide as the control. Mycelium disc and spores of Cladosporium musae, Mycosphaerella fijiensis and Deightoniella torulosa were put on the different media. Total inhibition of mycelium growth of every fungus on the PDA amended with propiconazole was observed. The fungicides used show different activities according to their concentration and their mode of action. The application of a fungicide should talke into account the pathogenic fungus involved in the leaf attacked. Against Mycosphaerella fijiensis var. difformis, the Ci(50) are different according to the concentrations (0.44 to 1.06 ppm). Correlations ranked from 0.71 to 0.91 are also different according to fungicide used. The Ci(50) of Cladosporium musae (0.10 to 2.44 ppm) and Deightoniella torulosa (0.26 to 0.52 ppm) are different and their correlations are respectively 0.78 to 0.99 and 0.86 to 0.95. An assessment of the sensitivity of parasitic fungi of banana can be made by mycelium growth measurement of fungi isolated from resistance zones.

  12. 40 CFR 81.252 - Northwest Kansas Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., Cheyenne County, Decatur County, Ellis County, Gove County, Graham County, Logan County, Ness County..., Sheridan County, Sherman County, Smith County, Thomas County, Trego County, Wallace County....

  13. 40 CFR 81.252 - Northwest Kansas Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., Cheyenne County, Decatur County, Ellis County, Gove County, Graham County, Logan County, Ness County..., Sheridan County, Sherman County, Smith County, Thomas County, Trego County, Wallace County....

  14. 40 CFR 81.252 - Northwest Kansas Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Cheyenne County, Decatur County, Ellis County, Gove County, Graham County, Logan County, Ness County..., Sheridan County, Sherman County, Smith County, Thomas County, Trego County, Wallace County....

  15. 40 CFR 81.252 - Northwest Kansas Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., Cheyenne County, Decatur County, Ellis County, Gove County, Graham County, Logan County, Ness County..., Sheridan County, Sherman County, Smith County, Thomas County, Trego County, Wallace County....

  16. 40 CFR 81.252 - Northwest Kansas Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Cheyenne County, Decatur County, Ellis County, Gove County, Graham County, Logan County, Ness County..., Sheridan County, Sherman County, Smith County, Thomas County, Trego County, Wallace County....

  17. Cantonese versus Canadian Evaluation of Directive and Non-Directive Therapy.

    ERIC Educational Resources Information Center

    Waxer, Peter H.

    1989-01-01

    Examined differences between Canadian and Cantonese university students who read transcripts of Carl Rogers and Albert Ellis counseling sessions and rated these counselors on directiveness, forcefulness, repetitiveness, sensitivity, politeness, and willingness to see either Ellis or Rogers. Found Canadians more willing to see Rogers than Chinese…

  18. Subject Characteristics and Preferences for Different Approaches to Psychotherapy: A Multivariate Study

    ERIC Educational Resources Information Center

    Helweg, Gregory C.; Gaines, Lawrence S.

    1977-01-01

    Selected personality variables, sex, age, interpersonal values, and educational level, were examined as antecedents to preferences for a nondirective (Carl Rogers) or directive (Albert Ellis) therapist. Individuals who preferred the Ellis presentation proved to more dogmatic and externalized than individuals preferring the Rogers presentation.…

  19. The Rational-Emotive Approach: A Critique

    ERIC Educational Resources Information Center

    Morris, G. Barry

    1976-01-01

    The critique of Rational-Emotive Therapy aims criticism at Ellis' concept of irrationality, analysis of human behavior and therapeutic techniques. Ellis suggests that his critic's claims lack the support of experimental evidence. He further suggests that an "existential" bias pervades which differs from his own brand of "existentialism." (KRP)

  20. 40 CFR 81.337 - Oklahoma.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Dewey County Ellis County Harper County Major County Roger Mills County Texas County Woods County... Harper County Major County Roger Mills County Texas County Woods County Woodward County AQCR 188... County Custer County Dewey County Ellis County Harper County Major County Roger Mills County Texas...

  1. "I'm Still Not Sure What a Task Is": Teachers Designing Language Tasks

    ERIC Educational Resources Information Center

    Erlam, Rosemary

    2016-01-01

    Ellis (2003) identifies four key criteria that distinguish a "task" from the types of situational grammar exercises that are typically found in the more traditional language classroom. This study investigates how well teachers were able to design tasks that fulfilled these four criteria (Ellis, 2003) at the end of a year-long…

  2. Measuring Implicit and Explicit Linguistic Knowledge: What Can Heritage Language Learners Contribute?

    ERIC Educational Resources Information Center

    Bowles, Melissa A.

    2011-01-01

    Although claims about explicit and implicit language knowledge are central to many debates in SLA, little research has been dedicated to measuring the two knowledge types (R. Ellis, 2004, 2005). The purpose of this study was to validate the use of the battery of tests reported in Ellis (2005) to measure implicit and explicit language knowledge.…

  3. Words, Hemispheres, and Processing Mechanisms: A Response to Marsolek and Deason (2007)

    ERIC Educational Resources Information Center

    Ellis, Andrew W.; Ansorge, Lydia; Lavidor, Michal

    2007-01-01

    Ellis, Ansorge and Lavidor (2007) [Ellis, A.W., Ansorge, L., & Lavidor, M. (2007). Words, hemispheres, and dissociable subsystems: The effects of exposure duration, case alternation, priming and continuity of form on word recognition in the left and right visual fields. "Brain and Language," 103, 292-303.] presented three experiments investigating…

  4. 76 FR 4724 - Catawba Sox, LLC Formerly Known as Catawba Sox, Inc. Including Workers Whose Unemployment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ... published in the Federal Register on August 2, 2010 (75 FR 45162). At the request of the State agency, the... Whose Unemployment Insurance UI) Wages Are Paid Through Ellis Hosiery Mill, LLC, Newton, NC; Amended... unemployment insurance (UI) tax account under the name Ellis Hosiery Mill, LLC, formerly known as Catawba...

  5. Finding a Balance: A Narrative Inquiry into Motherhood and the Doctoral Process

    ERIC Educational Resources Information Center

    Eisenbach, Brooke

    2013-01-01

    Carolyn Ellis states, "autoethnography shows struggle, passion, embodied life, and the collaborative creation of sense-making... [it] wants the reader to care, to feel, to empathize, and to do something, to act" (Ellis & Bochner, 2006, p. 433). This autoethnography describes one new mother's struggles to complete her doctoral program of study…

  6. Beyond Borders: Reading "Other" Places in Children's Literature

    ERIC Educational Resources Information Center

    Stewart, Susan Louise

    2008-01-01

    The author analyzes two texts, Gloria Whelan's "Homeless Bird" and Deborah Ellis's "Parvana's Journey", in an attempt to explain some of the problems and difficulties associated with those texts. The author examines Whelan's representations of India and finds troubling binaries associated with that text. In comparison, the author finds Ellis's…

  7. The Authors Reply

    ERIC Educational Resources Information Center

    Guilloteaux, Marie J.; Dornyei, Zoltan

    2009-01-01

    This article presents the authors' reply to Rod Ellis's comments on their study on motivated classroom behavior. As Ellis correctly summarizes, the three student variables selected for investigation in the authors' study were attention, participation, and volunteering for teacher-fronted activity. These three components were then summed up in a…

  8. A Guide to the Changing Court Rulings on Union Security in the Public Sector: A Management Perspective.

    ERIC Educational Resources Information Center

    Clark, R. Theodore, Jr.

    1985-01-01

    In "Ellis vs. Brotherhood of Railway, Airline and Steamship Clerks," the Supreme Court clarified the line between permissible and nonpermissible expenses and what procedures must be available to prevent compulsory subsidization of ideological activity by objecting employees. This article also describes the Court's decisions subsequent to "Ellis."…

  9. 75 FR 35776 - Sunshine Act Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-23

    ... of the public wishing to attend the meeting must notify Mary Lou Norris or Angela Ellis by c.o.b... Mary Lou Norris or Angela Ellis, and they will provide you with instructions for admittance. Non-U.S... Norris' e-mail address is marylou.norris@nist.gov , and her phone number is (301) 975-2002. Angela...

  10. 7 CFR 318.13-23 - Cut flowers from Hawaii.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION... of mauna loa and jade vine and except for cut blooms of gardenia not grown in accordance with... scale host plants is prohibited: Ixora, ginger (Alpinia purpurata), plumeria, coffee, rambutan,...

  11. 7 CFR 318.13-23 - Cut flowers from Hawaii.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION... of mauna loa and jade vine and except for cut blooms of gardenia not grown in accordance with... scale host plants is prohibited: Ixora, ginger (Alpinia purpurata), plumeria, coffee, rambutan,...

  12. 7 CFR 318.13-23 - Cut flowers from Hawaii.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION... of mauna loa and jade vine and except for cut blooms of gardenia not grown in accordance with... scale host plants is prohibited: Ixora, ginger (Alpinia purpurata), plumeria, coffee, rambutan,...

  13. 7 CFR 318.13-23 - Cut flowers from Hawaii.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION... of mauna loa and jade vine and except for cut blooms of gardenia not grown in accordance with... scale host plants is prohibited: Ixora, ginger (Alpinia purpurata), plumeria, coffee, rambutan,...

  14. Potential application for genipin-modified gelatin in leather processing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Genipin is a non-toxic iridoid compound, extracted from the gardenia fruits, and, because of its low cytoxicity, is replacing both glutaraldehyde and formaldehyde as a crosslinking reagent. In prior research we had shown the potential of chemical (glutaraldehyde) and enzyme (transglutaminase) modif...

  15. Potential application for genipin-modified gelatin in leather processing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Genipin is an iridoid compound extracted from gardenia fruits. Because of its low cytotoxicity, genipin can be used to replace both glutaraldehyde and formaldehyde as a crosslinking reagent. In recent years, research into the utilization of genipin for the modification of gelatin, particularly in ...

  16. 78 FR 74129 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-10

    ... Francisco, California 94105-1579: 1. John Jung Hun Chang, Wellwish Investment LLC, Ellis Eunrok Chang, all of Garden Grove, California, and Ellen Eunmi Chang, Bellevue, Washington; to retain voting shares...

  17. Personal Change and Intervention Style

    ERIC Educational Resources Information Center

    Andrews, John D. W.

    1977-01-01

    Presents a theory of personal change and analyzes growth-producing interventions using examples from the film, "Three Approaches to Psychotherapy". Compares the styles of Carl Rogers, Frttz Perls, and Albert Ellis to illustrate the theory. (Editor/RK)

  18. 77 FR 45925 - Standard Instrument Approach Procedures, and Takeoff Minimums and Obstacle Departure Procedures...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-02

    ... Intl 2/3666 7/11/12 RNAV (GPS) Y RWY 26, Amdt 3. 23-Aug-12 NC Jacksonville........ Albert J Ellis... Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of...

  19. The Counselor in a Nuclear World: A Rationale for Awareness and Action.

    ERIC Educational Resources Information Center

    Gearhart, Jo Anne

    1984-01-01

    Explores a rationale for counselors' responses to the threat of nuclear annihilation and suggests some action possibilities. Includes a response by Milton Schwebel emphasizing professional roles, and by Albert Ellis emphasizing the dangers of religous zealotism. (Author/JAC)

  20. An Emotional Control Card for Inappropriate and Appropriate Emotions in Using Rational-Emotive Imagery.

    ERIC Educational Resources Information Center

    Ellis, Albert

    1986-01-01

    Examines the emotional control card techniques developed by Sklare, Taylor, and Hyland (1985) to help clients more effectively use the rational-emotive imagery technique of Ellis (1974). Suggests a revision of the emotional control card technique. (NB)

  1. Widely spaced teeth

    MedlinePlus

    ... as a result of several diseases or continued growth of the jawbone. ... Ellis-van Creveld syndrome Injury Morquio syndrome Normal growth (temporary widening) Possible gum disease Sanfilippo syndrome Tooth shifting due to gum disease or missing teeth

  2. 2. GENERAL VIEW LOOKING EAST SHOWING SOUTH SIDE OF BRIDGE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. GENERAL VIEW LOOKING EAST SHOWING SOUTH SIDE OF BRIDGE. SHALLOW ARCH ON EXTREME RIGHT SPANS ELLIS ST. - Sudbury River Aqueduct, Echo Bridge, Spanning Charles River at Upper Newton Falls, Newton, Middlesex County, MA

  3. Flight restraint

    USGS Publications Warehouse

    Ellis, D.H.; Dein, F.J.; Ellis, D.H.; Gee, G.F.; Mirande, C.M.

    1996-01-01

    Many techniques are available for pre ve n ting escape of captive cranes. These include tenotomy, tenectomy, wing clipping, confinement under nets, amputation, brailing, and vane trimming (Ellis and Dein 1991). The advantages and limitations of each technique are presented.

  4. 7 CFR 319.37-2 - Prohibited articles.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... affecting § 319.37-2, see the List of CFR Sections Affected, which appears in the Finding Aids section of...) Austria, Finland, and Romania Stigmina deflectans (Karst) Ellis (Needlecast disease)....

  5. 7 CFR 319.37-2 - Prohibited articles.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...: For Federal Register citations affecting § 319.37-2, see the List of CFR Sections Affected, which... deflectans (Karst) Ellis (Needlecast disease). Europe Phacidiopycnis pseudotsuga (M. Wils.) Hahn (Douglas...

  6. 78 FR 25462 - Oklahoma; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... areas and Hazard Mitigation throughout the State. You are further authorized to provide snow assistance... Assistance. Dewey and Ellis Counties for snow assistance under the Public Assistance program for...

  7. A Guide to the Changing Court Rulings on Union Security in the Public Sector: A Union Perspective.

    ERIC Educational Resources Information Center

    Darko, Richard J.; Knapp, Janet C.

    1985-01-01

    The Supreme Court in "Ellis vs. Brotherhood of Railway, Airline and Steamship Clerks" has provided a systematic process for determining what constitutes union expenses properly charged to objecting nonmembers. (MLF)

  8. Interactions between a group of Golden Eagles and a herd of North American elk

    USGS Publications Warehouse

    O'Connell, Matt P.; Kochert, Michael N.

    2013-01-01

    Raptors are generally considered solitary predators (Schoener 1969), but occasionally they interact socially (Brown and Amadon 1968). Certain raptor species (e.g., Swallow-tailed Kites [Elanoides forficatus] and Swainson's Hawks [Buteo swainsoni]) concentrate in aggregations in response to localized, abundant food sources (Ellis et al. 1993). Many raptor species engage in group hunting (Ellis et al. 1993), and social foraging is a routine strategy for some species (e.g., Harris's Hawks [Parabuteo unicinctus]; Bednarz 1988, Ellis et al. 1993]. Raptors generally engage in group hunting to pursue elusive or large prey (Ellis et al. 1993). Occasionally individuals of conspecific raptors engage in play as a group sometimes involving chases of prey species (Palmer 1988). In this letter, we report interactions between a large group of Golden Eagles and a herd of adult and juvenile Rocky Mountain elk (Cervus canadensis nelsoni) in late autumn.

  9. Aeromagnetic maps of the Mazatzal Wilderness and contiguous roadless areas, Gila, Maricopa, and Yavapai counties, Arizona

    USGS Publications Warehouse

    Moss, C.K.; Abrams, G.A.

    1985-01-01

    Studies of the geology, geochemistry (Marsh and others, 1983a, b, Erickson, 1984), mines and prospects (Ellis, 1982), and mineral resource potential (Wrucke and others, 1983) of the Mazatzal Wilderness and contiguous roadless areas have been published elsewhere.

  10. A Theoretical and Empirical Integration of the Rational-Emotive and Classical Conditioning Theories

    ERIC Educational Resources Information Center

    Russell, Phillip L.; Brandsma, Jeffrey M.

    1974-01-01

    Galvanic skin conductance response, respiration rate and respiration depth values of an experimental and control group were used to test the hypotheses of a Albert Ellis' ABC Theory of psychopathology. (EK)

  11. Improving trends with pancreatoduodenectomy.

    PubMed

    Spratt, J S

    1976-02-01

    This paer stresses the complete change in outlook associated with pancreatoduodenectomy at the Ellis Fischel State Cancer Hospital since 1963, the year that marks the implementation of step-wise surgical principles espoused by Newton regarding the performance of pancreatoduodenectomy with the minimizing of morbidity and mortality factors. Since 1963 only one hospital death has occurred at Ellis Fishcel State Cancer Hospital after pancreatoduodenectomy and this case was atypical.

  12. Concentration of Inorganic Elements Content in Benthic Seaweeds of Fernando de Noronha Archipelago by Synchrotron Radiation Total Reflection X-Ray Fluorescence Analysis (SRTXRF)

    PubMed Central

    Ferreira, Leandro De Santis; Lopes, Rosana Peporine; Ulbrich, Mabel Norma Costas; Guaratini, Thais; Colepicolo, Pio; Lopes, Norberto Peporine; Garla, Ricardo Clapis; Oliveira Filho, Eurico Cabral; Pohlit, Adrian Martin; Zucchi, Orghêda Luiza Araújo Domingues

    2012-01-01

    SRTXRF was used to determine As, Ba, Br, Ca, Co, Cr, Cs, Cu, Dy, Fe, K, Mn, Mo, Ni, Pb, Rb, Sr, Ti, V, and Zn in eleven seaweed species commonly found in Fernando de Noronha: Caulerpa verticillata (J. Agardh) (Chlorophyta), Asparagopsis taxiformis (Delile), Dictyurus occidentalis (J. Agardh), Galaxaura rugosa (J. Ellis & Solander) J. V. Lamouroux, G. obtusata (J. Ellis & Solander) J. V. Lamouroux, G. marginata (J. Ellis & Solander) J. V. Lamouroux (Rhodophyta), Dictyota cervicornis (Kützing), Dictyopteris justii (J. V. Lamouroux), Dictyopteris plagiogramma (Montagne) Vickers, Padina gymnospora (Kützing) Sonder, and a Sargassum sp. (Phaeophyta). Data obtained were compared to those from the analysis of other parts of the world seaweeds using different analytical techniques and were found to be in general agreement in terms of major and minor elemental components. Results provide baseline information about the absorption and accumulation of these elements by macroalgae in the area. PMID:22505917

  13. Rational Emotive Behavioural Therapy: The Evolution of a Revolution

    PubMed Central

    Ellis, Debbie Joffe; Rovira, Montse

    2015-01-01

    Recognized as one of the most influential thinkers and psychologists, Albert Ellis PhD (1913-2007) revolutionized Psychology when he created the first cognitive psychotherapy, Rational Emotive Behavioral Therapy. After he passed away, Dr. Debbie Joffe Ellis continues spreading his legacy around the world. Psychologist, lecturer, writer, trainer, she dedicates her life to disseminate REBT and extend it through different statements, from the social to the educational, from the academic to the clinical. In this interview, she goes through her own history and her husband’s one, bringing us closer to understanding Albert Ellis as the leading figure in his field, and the oneness they experienced through their professional and personal relationship. PMID:27247638

  14. [Study on crocins reference extract and application on assay of Croci Stigma].

    PubMed

    He, Feng-yan; Dai, Zhong; He, Yi; Zhang, Yu-mei; Lu, Jing

    2015-06-01

    High price and poor stability of both crocin-1 and crocin-2 reference substance have become obstacles to HPLC assay of Croci Stigma. A new method based on reference extract was proposed. In this study, the reference extract was prepared from gardenia yellow which is cheap and easy to get The content of crocin-1 and crocin-2 in reference extract was determined and factors affecting stability of reference extract were investigated. Twelve batches of Croci Stigma were analyzed with reference extract and reference substance respectively. The results showed no difference. The presented method is feasible for quality control of Croci Stigma and reference extract is suitable to replace reference substances in assay.

  15. Academic Underachievement: A Reformulation and Rectification

    ERIC Educational Resources Information Center

    Sharma, Lal

    1972-01-01

    Numerous attempts have been made to rectify the kind of irrational factors which help prevent a young person from meeting one of society's first demands, that of satisfactory school performance. This paper discusses some of the existing literature on the subject, especially a rather effective technique suggested by Ellis. (Author)

  16. How Vietnamese Culture Influence on Learning and Teaching English

    ERIC Educational Resources Information Center

    Huong, Phan Thi Thu

    2008-01-01

    Vietnamese has to face a cross-culture issue with the teaching and learning of English as Vietnamese culture is "villagers' culture" which considers relationships in village as family relations and an emphasis "on hierarchical, social order in their dealings with one another" (Ellis, 1995: 9) with a traditional teaching method which is…

  17. Comprehensive Education Bolivarian-Style: The Alternative School in Barrio Pueblo Nuevo, Venezuela

    ERIC Educational Resources Information Center

    Cole, Mike

    2014-01-01

    In this article, the author traces revolutionary developments in an alternative school in Barrio Pueblo Nuevo, Mérida, in the Bolivarian Republic of Venezuela, a school that caters for students between 4 and 14. He begins by recounting some fieldwork done at the school on his behalf by Edward Ellis in 2010. He goes on to discuss a video made at…

  18. Teaching Second Language Acquisition Courses: Views from New Faculty

    ERIC Educational Resources Information Center

    Gorsuch, Greta J.; Beglar, David

    2004-01-01

    Second language acquisition (SLA) courses are a perennial feature of graduate level teacher preparation programs in Applied Linguistics and TESOL. While there has been recent interest in exploring the interfaces of second language acquisition research and classroom teaching (Ellis, 1997), the teaching of SLA courses at the university level is…

  19. 77 FR 24713 - Ocean Transportation Intermediary License Reissuance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-25

    ..., Woodbridge, NJ 07095. 022238F Grimes Supply Chain Services, March 31, 2012. Inc., 600 North Ellis Road... Ocean Transportation Intermediary License Reissuance Notice is hereby given that the following Ocean Transportation Intermediary licenses have been reissued by the Federal Maritime Commission pursuant to section...

  20. The Application of Humorous Song in EFL Classrooms and Its Effects on Listening Comprehension

    ERIC Educational Resources Information Center

    Rafiee, Marzieh; Kassaian, Zohre; Dastjerdi, Hossein Vahid

    2010-01-01

    Language learners need to feel secure and to be free of stress so they can focus on language tasks (Ellis, 1994). A language teacher should use different tools to encourage students and make them involved in learning process. Humor and song are effective tools, as they develop creativity and make the class environment an appropriate setting for…

  1. Implicit Knowledge, Explicit Knowledge, and Achievement in Second Language (L2) Spanish

    ERIC Educational Resources Information Center

    Gutierrez, Xavier

    2012-01-01

    Implicit and explicit knowledge of the second language (L2) are two central constructs in the field of second language acquisition (SLA). In recent years, there has been a renewed interest in obtaining valid and reliable measures of L2 learners' implicit and explicit knowledge (e.g., Bowles, 2011; R. Ellis, 2005). The purpose of the present study…

  2. Race, Reification, and Responsibility.

    ERIC Educational Resources Information Center

    Cancro, Robert

    Noting that many of the attacks on individual scientists as well as some of the attacks on the field of behavior genetics are more than intemperate--they are non-rational--the author discusses his experience as a signatory to a document drawn up by Ellis B. Page during the winter of 1971-1972. The intent of this controversial document was to…

  3. Nurturing Potential. Annual SAGE (Society for the Advancement of Gifted Education) Conference Proceedings (4th, Edmonton, Alberta, Canada, September 24-25, 1993).

    ERIC Educational Resources Information Center

    Calgary Univ. (Alberta). Centre for Gifted Education.

    This proceedings contains summaries of 24 conference papers focusing on new directions in gifted education. Each paper's summary is about four pages in length. Papers have the following titles and authors: "Supporting Giftedness in Girls in the Classroom" (Julia Ellis); "A Community of Learners in ECS" (Anne Green); "Inventive Momentum: Toward a…

  4. A Voice from the Past: Catharine Beecher's Response to Simone de Beauvoir

    ERIC Educational Resources Information Center

    Murphy, Madonna M.

    2002-01-01

    Charlesetta Ellis, President of the Midwest Philosophy of Education Society (2002-2004) gave the Presidential Address at the 2002 MPES Annual Meeting, November 8, 2002 at Chicago State University regarding the important contributions made by Simone de Beauvoir to education and especially the education of women. This paper is a response to the…

  5. Great Facts for September.

    ERIC Educational Resources Information Center

    Hansen, Karen L.

    1992-01-01

    Offers answers to questions on this issue's September calendar of events for elementary students. Items addressed include the Viking 2 spacecraft, children's poetry, Ellis Island, the space shuttle Discovery, space satellites and space junk, ice cream cones, Dr. Seuss, and the praying mantis. (SM)

  6. Interior detail, building 810, view to north showing curved chord ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior detail, building 810, view to north showing curved chord sections of roof trusses, 90mm lens plus electronic flash fill lighting. - Travis Air Force Base, B-36 Hangar, Between Woodskill Avenue & Ellis, adjacent to Taxiway V & W, Fairfield, Solano County, CA

  7. Using Stories in English Omani Curriculum

    ERIC Educational Resources Information Center

    Al Harrasi, Kothar Talib Sulaiman

    2012-01-01

    Learning a FL [foreign language] may be frustrating for some young learners; however, integrating fun in learning would encourage them to develop positive attitudes towards learning a language (Ellis & Brewster, 1991). Stories are an effective and enthusiastic technique in teaching young learners; they inject lots of amusement and help…

  8. 78 FR 56833 - Safety Zone; Fireworks Events in Captain of the Port New York Zone

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-16

    ..., 2011 (76 FR 69614). ] Table 1 1. A DMC Network Company, Briggs Launch site: A barge Fireworks, Ellis... a 360-yard radius from the barge. Date: September 9, 2013 (Rain Date: September 10, 2013). Time: 9..., 2013 (Rain Date: September 15, 2013). Time: 8:30 p.m.-10:00 p.m. 5. Circle Line Sightseeing...

  9. A Usage-Based Investigation of L2 Lexical Acquisition: The Role of Input and Output

    ERIC Educational Resources Information Center

    Crossley, Scott; Kyle, Kristopher; Salsbury, Thomas

    2016-01-01

    This study investigates relations between second language (L2) lexical input and output in terms of word information properties (i.e., lexical salience; Ellis, 2006a). The data for this study come from a longitudinal corpus of naturalistic spoken data between L2 learners and first language (L1) interlocutors collected over a year's time. The…

  10. Categories of Counselors Behavior as Defined from Cross-Validated Factoral Descriptions.

    ERIC Educational Resources Information Center

    Zimmer, Jules M.; And Others

    The intent of the study was to explore and categorize counselor responses. Three separate filmed presentations were shown. Participating with the same client were Albert Ellis, Frederick Perls, and Carl Rogers. At the beginning of each counselor statement, a number was inserted in sequence and remained on the videotape until completion of that…

  11. A Social Constructionist Position for Mental Health Counseling: A Reply to Jeffrey T. Guterman.

    ERIC Educational Resources Information Center

    Ellis, Albert

    1996-01-01

    Argues for an "and-also" format, rather than an "either-or" position, for social constructionist positions in mental health counseling. Ellis accepts the "grim reality" of mental illness, and clarifies his own recent "nonpositivist,""constructionist," and "even postmodernist" position in theory and practice. (EMK)

  12. A Continuation of the Dialogue on Issues in Counseling in the Postmodern Era.

    ERIC Educational Resources Information Center

    Ellis, Albert

    2000-01-01

    This is a continuation of the dialogue among Albert Ellis, Jeffrey T. Guterman, Earl Ginter, Sandra A. Rigazio-DiGilio, Allen E. Ivey, and Don C. Locke that has been appearing in the Journal of Mental Health Counseling on ethical issues of counseling in the postmodern era. Specifically addresses the ethical, constructivist, multicultural, and…

  13. Irrational Beliefs, Life Orientation and Temporal Perspective of Prison Inmates

    ERIC Educational Resources Information Center

    Morris, G. Barry; Zingle, Harvey

    1977-01-01

    This study investigated the psychological health of criminals based on Albert Ellis' Rational-Emotive Theory. Other dimensions of mental health examined were the criminals' life orientation, and temporal perspective. Findings indicated that the criminal sample possessed significantly more irrational beliefs and were less future oriented than…

  14. A Bridge Too Far: Incompatibilities of Rational-Emotive Therapy and Pastoral Counseling.

    ERIC Educational Resources Information Center

    Wessler, Richard L.

    1984-01-01

    Suggests that pure rational-emotive therapy (RET) is inappropriate for pastoral counseling and proposes a modified version (cognitive appraisal therapy). In a reply, Albert Ellis clarifies his views of religion and contends that RET is clearly acceptable to many clergymen. (JAC)

  15. Rational Emotive Education

    ERIC Educational Resources Information Center

    Knaus, William

    1977-01-01

    Rational Emotive Education--an outgrowth of theories developed by Albert Ellis--is a teaching design of mental health concepts and problem-solving activities designed to help students to approach and cope with their problems through experiential learning, via a structured, thematic sequence of emotive education lessons. (MJB)

  16. Theories of Counseling: Their Relevancy to Non-Traditional Students.

    ERIC Educational Resources Information Center

    Gutierrez, George A.

    This report presents theories in counseling not merely as abstract thoughts, but as beliefs and proposed procedures to follow in helping a client when he/she has difficulties coping with life. Four main counseling theories are then described: (1) Client-Centered Counseling (Carl Rogers); (2) Rational-Emotive Therapy (Albert Ellis); (3) Reality…

  17. Beyond Cumulative Risk: Distinguishing Harshness and Unpredictability as Determinants of Parenting and Early Life History Strategy

    ERIC Educational Resources Information Center

    Belsky, Jay; Schlomer, Gabriel L.; Ellis, Bruce J.

    2012-01-01

    Drawing on life history theory, Ellis and associates' (2009) recent across- and within-species analysis of ecological effects on reproductive development highlighted two fundamental dimensions of environmental variation and influence: harshness and unpredictability. To evaluate the unique contributions of these factors, the authors of present…

  18. Learning Style and Task Performance in Synchronous Computer-Mediated Communication: A Case Study of Iranian EFL Learners

    ERIC Educational Resources Information Center

    Hedayati, Mohsen; Foomani, Elham Mohammadi

    2015-01-01

    The study reported here explores whether English as a foreign Language (EFL) learners' preferred ways of learning (i.e., learning styles) affect their task performance in computer-mediated communication (CMC). As Ellis (2010) points out, while the increasing use of different sorts of technology is witnessed in language learning contexts, it is…

  19. The Linguistically Aware Teacher and the Teacher-Aware Linguist

    ERIC Educational Resources Information Center

    McCartney, Elspeth; Ellis, Sue

    2013-01-01

    This review evaluates issues of teacher linguistic knowledge relating to their work with children with speech, language and communication difficulties (SLCD). Information is from Ellis and McCartney [(2011a). "Applied linguistics and primary school teaching." Cambridge: Cambridge University Press], a state-of-the-art text deriving from a British…

  20. Genetics Home Reference: familial adenomatous polyposis

    MedlinePlus

    ... R215-6. Review. Citation on PubMed Cheadle JP, Sampson JR. Exposing the MYtH about base excision repair ... 21(20):2525-38. Review. Citation on PubMed Sampson JR, Dolwani S, Jones S, Eccles D, Ellis A, Evans ...

  1. First report of powdery mildew caused by Podosphaera leucotricha on Callery pear in North America

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Podosphaera leucotricha (Ellis & Everh.) E.S. Salmon (Ascomycetes, Erysiphales) is the etiological agent of a powdery mildew disease that occurs on rosaceous plants, primarily Malus and Pyrus. This fungus is nearly circumglobal. In May 2009, leaves of Bradford pear (Pyrus calleryana Decne.), some di...

  2. Fostering Emotional Adjustment among Nigerian Adolescents with Rational Emotive Behaviour Therapy

    ERIC Educational Resources Information Center

    Adomeh, Ilu O. C.

    2006-01-01

    This study examined the efficacy of Albert Ellis' Rational Emotive Behaviour Therapy (REBT) in fostering emotional adjustment among Nigerian adolescents. Fifty senior secondary school students were randomly selected and divided equally into experimental and control groups. The experimental group was treated with REBT twice a week for six weeks.…

  3. Alleviating Communication Apprehension through Rational Emotive Therapy: A Comparative Evaluation.

    ERIC Educational Resources Information Center

    Watson, Arden K.; Dodd, Carley H.

    Albert Ellis's Rational Emotive Therapy (RET), which assumes that a person can change an emotional disturbance by discovering and disputing the irrational ideas giving rise to that emotion, has been used effectively in treating public speaking anxiety. To compare RET with other treatments for communication apprehension, 52 high communication…

  4. Rational Emotive Behavior Therapy with Troubled Students.

    ERIC Educational Resources Information Center

    Zionts, Paul; Zionts, Laura

    1997-01-01

    Based on the early work of Albert Ellis, seeks to identify and challenge irrational beliefs that underlie behavior problems. Outlines concepts and methods of Rational Emotive Behavior Theory and describes the application both in counseling and as a mental health curriculum for troubled children and youth. Offers classroom techniques. (RJM)

  5. If You Want to Feel Better--Try Thinking Better.

    ERIC Educational Resources Information Center

    Rogers, George W., Jr.

    This article briefly describes Rational Emotive or Rational Behavior Therapy. Some historical data is given regarding its developer, Dr. Albert Ellis, and his beliefs. In addition the following rational concepts are examined: (1) the anatomy of an emotion; (2) the characteristics of rational thoughts; and (3) the rational self-analysis of…

  6. Rational Emotive Behavior Therapy: Origins, Constructs, and Applications.

    ERIC Educational Resources Information Center

    Watson, Joshua C.

    In 1956, Dr. Albert Ellis presented his seminal work on Rational Therapy, subsequently renamed Rational Emotive Behavior Therapy (REBT) in 1993. This paper explores the origins, theoretical foundations, applications, and implications of REBT and provides a look at the empirical research available in support of the approach's efficacy. REBT is…

  7. Rational Emotive Education and the Developmental Stages of Jean Piaget.

    ERIC Educational Resources Information Center

    Lang, Janet M.

    Rational-Emotive Therapy (RET) is predicated on a theory of causality. According to Ellis (1962), beliefs regarding an event, and not the event itself, cause emotional reactions. Mentally healthy persons practice this reational theory of causality. Neurotic persons accept an irrational theory of causality based on coincidental or correlational…

  8. Action potential and contraction of Dionaea muscipula (Venus flytrap).

    PubMed

    DI PALMA, J R; MOHL, R; BEST, W

    1961-03-24

    Observation of the action potential and contraction of the leaf of Dionaea muscipula Ellis revealed several interesting phenomena. Two successive stimuli are generally necessary to cause contraction. The first and ineffective stimulus is associated with slow depolarization. The second stimulus has much more rapid depolarization and initiates contraction.

  9. Prevalence of Traumatic Dental Injuries in Patients Attending University of Alberta Emergency Clinic

    PubMed Central

    Alkhadra, Thamer; Preshing, William; El-Bialy, Tarek

    2016-01-01

    Objectives: This study evaluated the prevalence of dental trauma for patients attending the emergency dental clinic at the University of Alberta Hospital between 2006-2009. Patients’ examination and treatment charts were reviewed. Methods: Total number of patients’ charts was 1893.The prevalence of different types of trauma was 6.4 % of the total cases (117 patients). Trauma cases were identified according to Ellis classification and as modified by Holland et al., 1988. Results: Logistic statistical model showed that 21.7% were Ellis class I trauma, 16.7% were Ellis class II trauma, and 6.7% were Ellis class III. In addition, 11.7 % presented with avulsion, 7.5 % presented with dentoalveolar fracture and 7.5% presented with sublaxation. Also, 17.55 % presented with tooth displacement within the alveolar bone, 3.3 % presented with crown fracture with no pulp involvement, 4.16 % presented with crown fracture with pulp involvement and 3.3 % presented with root fracture. In conclusion, the general prevalence of dentoalveolar trauma in patients attending the emergency clinic at the University of Alberta is less than other reported percentages in Canada or other countries. PMID:27398104

  10. The Impact of an Institutional Black Male Leadership Initiative on Engagement and Persistence

    ERIC Educational Resources Information Center

    Barker, Marco J.; Avery, Jared C.

    2012-01-01

    While there has been growing research that explores the impact of race- and cultural-specific student organizations or spaces on Black students and particularly Black males (Dancy, 2011; Harper & Quaye, 2007; McClure, 2006; Museus, 2008; L. Patton, 2006), there have been fewer studies (Ellis, 2009) examining emerging, institutionalized Black male…

  11. 41. Photograph of a line drawing. '3'0' x 6'0' x ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    41. Photograph of a line drawing. '3'-0' x 6'-0' x 2'-0' WOODEN NUTSCHE, BUILDING G.' Holston Ordnance Works, Tennessee Eastman Corporation. September 24, 1942. Delineator: E. E. Ellis. Drawing # 7651-1007-218. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

  12. The Experience of Daily Hassles, Cardiovascular Reactivity and Adolescent Risk Taking and Self-Esteem

    ERIC Educational Resources Information Center

    Vermeersch, Hans; T'Sjoen, Guy; Kaufman, Jean-Marc; Vincke, John; Bracke, Piet

    2010-01-01

    Based on Boyce and Ellis's model on "context" and "biological sensitivity to the context", this article analyzes the interaction between the experience of daily hassles and experimentally induced cardiovascular reactivity as an indicator of stress reactivity, in explaining risk taking and self-esteem. This study found, in a sample of 599…

  13. Moving Business and Marketing Education into the 21st Century. Proceedings of the Annual Meeting of the Atlantic Coast Business and Marketing Education Conference (17th, Raleigh, North Carolina, February 18-19, 2000).

    ERIC Educational Resources Information Center

    Parke, David, Ed.

    This document contains 21 presentations from a conference on business and marketing education. The following papers are included: "Microsoft Excel 2000" (Jeff Fuller); "Clueless in the Classroom? Hints To Help!" (Mary W. Evans); "A Strategy To Improve Narrative-Number Linkage in Business Writing" (Ellis A. Hayes); "Corporate View: Bringing Realism…

  14. Multidimensional Perception of Counselor Behavior

    ERIC Educational Resources Information Center

    Barak, Azy; LaCrosse, Michael B.

    1975-01-01

    Investigated Strong's prediction of the existence of three dimensions of perceived counselor behavior--expertness, attractiveness, and trustworthiness. Films of interviews given by Rogers, Ellis, and Perls were watched by 202 subjects, who rated each counselor on 36 bipolar scales. Results supported the existence of the hypothesized dimensions for…

  15. Building Bridge between Learning and Positive Emotion: How to Apply Emotional Factor in Instructional Designing Process?

    ERIC Educational Resources Information Center

    Park, Sanghoon

    2004-01-01

    For millennia, emotional states have been viewed as avoidable impediments to rational thinking (Ellis & Newton, 2000). Several reasons have been pointed out. The lack of consensus of the definition on emotion that tend to conflict with each other was suggested as a main reason (Price, 1998). Also the difficulty of research methodology such as…

  16. Smart Books: Thinking with a Purpose Across the Curriculum. Media Corner.

    ERIC Educational Resources Information Center

    Coleman, Rhoda, Ed.

    1995-01-01

    Presents reviews of four CD-ROM products developed by Scholastic Books, Inc. Maintains that each of the programs makes extensive use of primary sources and heighten student interest. Discusses products that cover Greek mythology, the sinking of the "Titanic," Malcolm X, and immigrants at Ellis Island. (CFR)

  17. Draft Genome Sequence of the Psychrophilic Gliding Species Gelidibacter algens ACAM 536

    PubMed Central

    2016-01-01

    A draft genome sequence was obtained from the type strain of Gelidibacter algens (ACAM 536). This species was isolated from sea-ice diatom assemblages collected from Ellis Fjord, Eastern Antarctica. The genome of ACAM 536 is a single circular chromosome with an estimated size of 4.50 Mbp. PMID:27587822

  18. Pedagogical Moments: Affective Sexual Literacies in Film

    ERIC Educational Resources Information Center

    Clarke, Kyra

    2013-01-01

    This paper considers three pedagogical moments in the film "Tomorrow, When the War Began" (2010), contemplating the way in which they open a space for conversations about feelings, sexuality and gender. "Tomorrow, When the War Began" follows the plight of 17-year-old Ellie who returns to her rural town from a camping trip with…

  19. Traversable intra-Universe wormholes and timeholes in General Relativity: two new solutions

    NASA Astrophysics Data System (ADS)

    Smirnov, Alexey L.

    2016-11-01

    Using thin shell formalism we construct two solutions of intra-Universe wormholes. The first model is a cosmological analog of the Aichelburg–Schein timehole, while another one is an intra-Universe form of the Bronnikov–Ellis solution.

  20. The Long Run

    ERIC Educational Resources Information Center

    Donnelly, Laura

    2007-01-01

    This article profiles six educators who have taught for as long as two generations. These educators are the following: (1) Hazel Wilson--teaching 47 years; (2) Agnes Zeiger--teaching 61 years; Rose Gilbert--teaching 51 years; (4) Ellie Johnson--teaching 52 years; (5) Roy Clare--teaching 48 years; and (6) Carrie Hansen--54 years in education. These…

  1. Journal of Business and Training Education, 2000.

    ERIC Educational Resources Information Center

    Redmann, Donna H., Ed.

    2000-01-01

    This ninth issue contains seven articles dealing with research, theory, trends and issues, curriculum, teaching methodology, technology, and personal/professional development. "Attitudes of FBLA (Future Business Leaders of America) Students toward Traditional Business Values and the American Economic System" (Inder P. Nijihawan, Richard S. Ellis)…

  2. 76 FR 38414 - James Campbell National Wildlife Refuge, Honolulu County, HI; Draft Comprehensive Conservation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-30

    ... the Federal Register on December 1, 2008 (74 FR 8564), announcing our intention to complete a CCP/EA... Fish and Wildlife Service James Campbell National Wildlife Refuge, Honolulu County, HI; Draft... Highway, Room 2C, Hale`iwa, HI 96712. FOR FURTHER INFORMATION CONTACT: David Ellis, Project Leader,...

  3. Screening of microbial volatile organic compounds for detection of disease in cattle: development of lab-scale method

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The quest to find unique marker volatile organic compounds (VOCs) associated with human, livestock and wildlife diseases (Ellis et al., 2014) requires development of diagnostic non-invasive point-of-care tools and field surveillance technologies and strategies. The objective of this research was to ...

  4. Mixed Media: A Roundup of CD-ROM and Electronic Products.

    ERIC Educational Resources Information Center

    Bruno, Frank Alan

    2001-01-01

    Highlights multicultural materials that are useful for teaching students of all ages (elementary through college level). These include such CD-ROM products as "The Ellis Island Experience" and "The Civil Rights Movement in the United States" and such World Wide Web-based products as "Diversify Your World,""American Slavery: A Complete…

  5. The Federal Government's Relationship to the Nationally Recognized Accrediting Agencies.

    ERIC Educational Resources Information Center

    Office of Education (DHEW), Washington, DC.

    Proceedings include: the keynote address (John Ellis); themes and questions on accreditation and institutional eligibility (David A. Trivett); the task force on futuristic Office of Education criteria for recognition (Samuel P. Martin); possible accreditation agency uses of the products of the Office of Education project on improving the consumer…

  6. Evolution, Stress, and Sensitive Periods: The Influence of Unpredictability in Early versus Late Childhood on Sex and Risky Behavior

    ERIC Educational Resources Information Center

    Simpson, Jeffry A.; Griskevicius, Vladas; Kuo, Sally I-Chun; Sung, Sooyeon; Collins, W. Andrew

    2012-01-01

    According to a recent evolutionary life history model of development proposed by Ellis, Figueredo, Brumbach, and Schlomer (2009), growing up in harsh versus unpredictable environments should have unique effects on life history strategies in adulthood. Using data from the Minnesota Longitudinal Study of Risk and Adaptation, we tested how harshness…

  7. The Argument-Structure Complexity Effect in Children with Specific Language Impairment: Evidence from the Use of Grammatical Morphemes in French

    ERIC Educational Resources Information Center

    Pizzioli, Fabrizio; Schelstraete, Marie-Anne

    2008-01-01

    Purpose: The hypothesis that the linguistic deficit presented by children with specific language impairment (SLI) is caused by limited cognitive resources (e.g., S. Ellis Weismer & L. Hesketh, 1996) was tested against the hypothesis of a limitation in linguistic knowledge (e.g., M. L. Rice, K. Wexler, & P. Cleave, 1995). Method: The study examined…

  8. Working-Class Women in the Academy: Laborers in the Knowledge Factory.

    ERIC Educational Resources Information Center

    Tokarczyk, Michelle M., Ed.; Fay, Elizabeth A., Ed.

    This volume contains a collection of essays on the issues and concerns that face women from working-class backgrounds who enter academic careers. Following an introduction and transcript of a dialogue between Kate Ellis and Lillain S. Robinson, the essays are as follows: "What's a Nice Working-Class Girl Like You Doing in a Place Like This?"…

  9. Response to Pienemann's Critique of Zhang and Lantolf (2015)

    ERIC Educational Resources Information Center

    Lantolf, James P.; Zhang, Xian

    2015-01-01

    We respond here to Pienemann's critique of our study that appeared earlier this year in the Language Learning Special Issue entitled "Orders and Sequences in the Acquisition of L2 Morphosyntax, 40 Years On" and guest edited by Jan Hulstijn, Rod Ellis, and Søren Eskildsen. Pienemann objected to our claim that the Teachability Hypothesis…

  10. Clients as Teachers.

    ERIC Educational Resources Information Center

    Journal of Counseling and Development, 1988

    1988-01-01

    Contains eight accounts: "But Kids Don't Commit Suicide" (Sharon Andreozzi); "Learning from Multiplicity" (Kathryn Ellis); "Autonomy, Control, and Affiliation: Your Issues or Mine?" (Claudia Haferkamp); "Role Players" (Dennis Heitzmann); "Grief Counseling: Facilitating the Healing Process" (Rosemary Hughes); "Hospital Referral: A Test of Counselor…

  11. From Seeing Adverbs to Seeing Verbal Morphology: Language Experience and Adult Acquisition of L2 Tense

    ERIC Educational Resources Information Center

    Sagarra, Nuria; Ellis, Nick C.

    2013-01-01

    Adult learners have persistent difficulty processing second language (L2) inflectional morphology. We investigate associative learning explanations that involve the blocking of later experienced cues by earlier learned ones in the first language (L1; i.e., transfer) and the L2 (i.e., proficiency). Sagarra (2008) and Ellis and Sagarra (2010b) found…

  12. Learned Attention in Adult Language Acquisition: A Replication and Generalization Study and Meta-Analysis

    ERIC Educational Resources Information Center

    Ellis, Nick C.; Sagarra, Nuria

    2011-01-01

    This study investigates associative learning explanations of the limited attainment of adult compared to child language acquisition in terms of learned attention to cues. It replicates and extends Ellis and Sagarra (2010) in demonstrating short- and long-term learned attention in the acquisition of temporal reference in Latin. In Experiment 1,…

  13. Keeping Black Poetry Alive

    ERIC Educational Resources Information Center

    Mehta, Diane

    2006-01-01

    Thomas Sayers Ellis, assistant professor of creative writing at New York's Sarah Lawrence College, is one of many scholars fighting for the soul of Black poetry, a struggle that takes place largely off-campus. Unless one is accepted into a top-level graduate poetry program, such as Boston University's program or the Iowa Writing Workshop, a poet's…

  14. Point of Entry

    ERIC Educational Resources Information Center

    Manzo, Kathleen Kennedy

    2007-01-01

    As part of a professional development program organized by the Save Ellis Island Foundation, the exhibits, databases, photo archives, and recorded interviews at the island's museum helps put the nation's current immigration debate into a broader historical context. Teachers at these sessions learn from scholars and park personnel about early…

  15. Gifted Programs.

    ERIC Educational Resources Information Center

    Roeper Review, 1979

    1979-01-01

    Descriptions of four gifted programs are offered in articles titled "Career Education for Gifted Students in Illinois" (J. Ellis and B. Ford); "Interage Program for Critical Thinking" (H. Masterson); "Talcott Mountain Science Center" (D. LaSalle); and "Project L.O.G.I.C.--Nurturing the Mathematically Gifted" (W. Foley and B. Spack). (SBH)

  16. Prescriptions: Hyperrealism and the Chemical Regulation of Mood

    ERIC Educational Resources Information Center

    Bleakley, Alan; Jolly, Margaretta

    2012-01-01

    Using contemporary literary sources, we explore the powerful ideological framework that normalises prescription dependency as part of everyday life, focusing upon the treatment of mood disorders. Through a literary critical methodology, we read novels by American hyperrealists such as Bret Easton Ellis, David Foster Wallace and Rick Moody as…

  17. Testing a Threshold: An Approximate Replication of Johnson, Mercado & Acevedo 2012

    ERIC Educational Resources Information Center

    Johnson, Mark D.; Nicodemus, Christine L.

    2016-01-01

    In order to better understand the role of working memory in second language (L2) written production, this study contributes to recent research attempting to apply Kellogg's model of working memory in first language (L1) writing to L2 writing research (Ellis & Yuan 2004; Ong & Zhang 2010; Johnson, Mercado & Acevedo 2012). This paper…

  18. Elevations and Floor Plan of Shed No. 1, Elevations and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Elevations and Floor Plan of Shed No. 1, Elevations and Floor Plan of Work Shed, Elevations and Floor Plan of Garage - Roberts-Dolezal Farmstead, 75 miles northeast of the intersection of CR27 and FM 1722, Garrett, Ellis County, TX

  19. Effects of Pretask Modeling on Attention to Form and Question Development

    ERIC Educational Resources Information Center

    Kim, YouJin

    2013-01-01

    Over the last two decades, a growing body of research has shown positive impacts for task planning in task-based instruction (e.g., Ellis, 2005; Foster & Skehan, 1996). However, what learners plan during pretask planning, and whether any specific planning strategies are more beneficial in encouraging learners to attend to linguistic forms and…

  20. A Prosodically Controlled Word and Nonword Repetition Task for 2- To 4-Year-Olds: Evidence from Typically Developing Children.

    ERIC Educational Resources Information Center

    Roy, Penny; Chiat, Shula

    2004-01-01

    An association has been found between nonword repetition and language skills in school-age children with both typical and atypical language development (C. Dollaghan & T. F. Campbell, 1998; S. Ellis Weismer et al., 2000; S. E. Gathercole & A. D. Baddeley, 1990; J. W. Montgomery, 2002). This raises the possibility that younger children's repetition…

  1. Schools Reaching Out: An Introduction.

    ERIC Educational Resources Information Center

    Heleen, Owen

    1990-01-01

    Introduces an issue devoted to the Schools Reaching Out projects at David A. Ellis School in Roxbury (MA) and Adolph S. Ochs School in New York City. Projects attempt to link the schools with parents and their communities to promote the students' academic and social success. (DM)

  2. 6. VIEW OF BRIDGE COMMEMORATIVE PLAQUE WHICH STATES '1908, J. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW OF BRIDGE COMMEMORATIVE PLAQUE WHICH STATES '1908, J. H. CROOKS, ED. ELLIS, A. S. LELAND, COMMISSIONERS. L. E. BRELSFORD, AUDITOR. L. WEST, SURVEYOR. - B. C. GERWICK, DESIGNER. F. E. WITHCOTT, ENG. ON CONST. C. A. WARNER, CONTRACTOR.' - First Street Reinforced Concrete Bridge, Spanning Moxahala Creek at First Street (CR 7), Roseville, Muskingum County, OH

  3. Is the Acquisition Order of Grammatical Morphemes Impervious to L1 Knowledge? Evidence from the Acquisition of Plural -"s", Articles, and Possessive '"s"

    ERIC Educational Resources Information Center

    Luk, Zoe Pei-sui; Shirai, Yasuhiro

    2009-01-01

    In SLA, it has been often assumed that the effect of the first language (L1) is not very strong in the acquisition of grammatical morphemes (e.g., Ellis, 1994; Mitchell & Myles, 2004). However, such an assumption has not been systematically examined in the literature. This article reviews the morpheme studies conducted with native speakers of…

  4. Irrational Beliefs of Shoplifters.

    ERIC Educational Resources Information Center

    Solomon, Gary S.; Ray, Joseph B.

    1984-01-01

    Developed and tested the Shoplifter Offenders Program (SOP) which uses the principles of Ellis' rational-emotive therapy (RET) as the conceptual foundation for the intensive, 8-hour, psychoeducational group counseling experience with adult first offender shoplifters (N=94). Results showed a recidivism rate of approximately one percent. (LLL)

  5. The Intimate Correlation of Invitational Education and Effective Classroom Management

    ERIC Educational Resources Information Center

    Riner, Phillip S.

    2003-01-01

    Critics of Invitational Education and other self-concept approaches to learning have long argued that there is a lack of empirical data to support the claims that approaches to student instruction based on self-concept theory are central to effective learning. Ellis (2001) examines a number of these analyses where self-concept, self-esteem, and…

  6. The Introductory Mathematics Curriculum: Misleading, Outdated, and Unfair and Responses .

    ERIC Educational Resources Information Center

    Roberts, Fred S.; And Others

    1984-01-01

    Roberts discusses his perception that the college introductory curriculum in mathematics is outdated. Reactions are then presented from Lucas, Hamming, Tall, David, Ellis, Thompson, Mason, and Guy, with a response by Roberts. The need to change instructional methods as well as curriculum is noted by most. (MNS)

  7. The Contribution of Domain-Specific Knowledge in Predicting Students' Proportional Word Problem Solving Performance

    ERIC Educational Resources Information Center

    Jitendra, Asha K.; Lein, Amy E.; Star, Jon R.; Dupuis, Danielle N.

    2013-01-01

    Proportional thinking, which requires understanding fractions, ratios, and proportions, is an area of mathematics that is cognitively challenging for many children and adolescents (Fujimura, 2001; Lamon, 2007; Lobato, Ellis, Charles, & Zbiek, 2010; National Mathematics Advisory Panel [NMAP], 2008) and "transcends topical barriers in adult…

  8. Opulence to Decadence: "The Outsiders" and "Less than Zero."

    ERIC Educational Resources Information Center

    Seay, Ellen A.

    1987-01-01

    Compares and contrasts two adolescent novels, S. E. Hinton's "The Outsiders," and B. Ellis' recent "Less than Zero." Argues for the social relevance and educational worth of both novels, showing that they raise moral and social issues teens need to think about and are already confronting. (JG)

  9. Verbal Working Memory in Bilingual Children.

    ERIC Educational Resources Information Center

    Gutierrez-Clellen, Vera F.; Calderon, Janet; Weismer, Susan Ellis

    2004-01-01

    The present study compared the performance of 44 Latino children on the Competing Language Processing Task (CLPT; C. Gaulin & T. Campbell, 1994) and the Dual Processing Comprehension Task (DPCT; S. Ellis Weismer, 1996). First, it was of interest to know if there were significant differences between children with and without bilingual proficiency…

  10. Schools Reaching Out and Success for All Children: Two Case Studies.

    ERIC Educational Resources Information Center

    Swap, Susan McAllister

    This research report evaluates a 2-year effort to achieve home-school partnerships and success for all children at two urban schools: P.S. 111 in New York City and the Ellis School in Boston. As conceptualized by the Institute for Responsive Education staff, the process of achieving success for all children required the transformation of school…

  11. Second Language Learners' Contiguous and Discontiguous Multi-Word Unit Use over Time

    ERIC Educational Resources Information Center

    Yuldashev, Aziz; Fernandez, Julieta; Thorne, Steven L.

    2013-01-01

    Research has described the key role of formulaic language use in both written and spoken communication (Schmitt, 2004; Wray, 2002), as well as in relation to L2 learning (Ellis, Simpson--Vlach, & Maynard, 2008). Relatively few studies have examined related fixed and semi-fixed multi-word units (MWUs), which comprise fixed parts with the potential…

  12. Motivating the Gifted, Talented, Creative. A Meta-Analysis.

    ERIC Educational Resources Information Center

    Shaughnessy, Michael F.

    The main theories of motivation are reviewed in an effort to help teachers discern various tactics and techniques that they can use with gifted, talented, and creative students. Among the theories examined are Ivan Pavlov's respondent conditioning; Albert Ellis's rational-emotive approach; Abraham Maslow's triangular approach to growth and…

  13. The Relationship between Teachers' Beliefs of Grammar Instruction and Classroom Practices in the Saudi Context

    ERIC Educational Resources Information Center

    Alghanmi, Bayan; Shukri, Nadia

    2016-01-01

    Teacher cognition (Borg, 2015) of grammar instruction is a relatively new phenomenon that has yet to be explored in the Saudi context. While many studies have focused on the teaching of grammar in general (Ellis, 2006; Corzo, 2013; Braine, 2014), further research needs to be done - particularly when it comes to understanding teachers' beliefs of…

  14. Exploring the Potential of Rehearsal via Automatized Structured Tasks versus Face-to-Face Pair Work to Facilitate Pragmatic and Oral Development

    ERIC Educational Resources Information Center

    Sydorenko, Tetyana V.

    2011-01-01

    Past research has uncovered ways to improve communicative competence, including task-based learner-learner interaction (e.g., R. Ellis, 2003) and task planning (e.g., Mochizuki and Ortega, 2008). Teacher-guided planning particularly increases the benefits of learner-learner interaction (Foster and Skehan, 1999). One component of communicative…

  15. Generalizing: The Core of Algebraic Thinking

    ERIC Educational Resources Information Center

    Kinach, Barbara M.

    2014-01-01

    Generalizing--along with conjecturing, representing, justifying, and refuting--are forms of mathematical reasoning important in all branches of mathematics (Lannin, Ellis, and Elliott 2011). Increasingly, however, generalizing is recognized as the essence of thinking in algebra (Mason, Graham, and Johnston-Wilder 2010; Kaput, Carraher, and Blanton…

  16. Missionary Girl Power: Saving the "Third World" One Girl at a Time

    ERIC Educational Resources Information Center

    Sensoy, Ozlem; Marshall, Elizabeth

    2010-01-01

    Deborah Ellis's "The Breadwinner" is a popular young adult novel about Muslim girls. In this paper, we offer an analysis of the representation of Muslim girls and women in the book as well as responses from undergraduate students enrolled in a children's literature course to these constructions. Building on the work of postcolonial feminism…

  17. 78 FR 33464 - Texas Disaster #TX-00405

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-04

    ... ADMINISTRATION Texas Disaster TX-00405 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Texas dated 05/29/2013... adversely affected by the disaster: Primary Counties: Ellis, Hood, Johnson. Contiguous Counties:...

  18. Facing the Future.

    ERIC Educational Resources Information Center

    Gallin, Alice, Ed.

    1983-01-01

    Ways that Catholic colleges and universities are trying to fulfill their role are discussed in articles by six college presidents and a church historian. In "The Catholic Liberal Art College: Has It a Future?" John Tracy Ellis notes some of the roots to be reaffirmed by Catholic colleges. In "Preparing for the Millennium," Theodore M. Hesburgh…

  19. The Potential Importance of Conservation, Restoration and Altered Management Practices for Water Quality in the Wabash River Watershed

    EPA Science Inventory

    The Potential Importance of Conservation, Restoration and Altered Management Practices for Water Quality in the Wabash River Watershed Guoxiang Yang1, Elly P.H. Best2, Staci Goodwin3 1 ORISE Postdoc Research Associate at U.S. Environmental Protection Agency, National Risk...

  20. Impact of Consciousness-Raising Activities on Young English Language Learners' Grammar Performance

    ERIC Educational Resources Information Center

    Fatemipour, Hamidreza; Hemmati, Shiva

    2015-01-01

    Grammar Consciousness-Raising (GCR) is an approach to teaching of grammar which learners instead of being taught the given rules, experience language data. The data challenge them to rethink, restructure their existing mental grammar and construct an explicit rule to describe the grammatical feature which the data illustrate (Ellis, 2002). And…

  1. The Fight over Screening Students to Prevent Suicide

    ERIC Educational Resources Information Center

    Ashford, Ellie

    2005-01-01

    An article by Ellie Ashford condensed from School Board News (Jun 28, 2005) is presented in this article. Proponents of mental health screening point to stories to illustrate the benefits of screening seemingly healthy teenagers. Yet, opposition to mental health screening is growing, with one school health expert calling it "as controversial as…

  2. 21st Century Learning and Information Literacy

    ERIC Educational Resources Information Center

    Breivik, Patricia Senn

    2005-01-01

    The faces of immigrant children who had come through Ellis Island with their parents in search of better futures stare out at me from a set of black and white photographs near my desk. The children, frozen in time, are lined up for blocks, waiting for the doors of a public library to open. Access to information was their pathway to a future of…

  3. Information-Seeking Behavior in the Digital Age: A Multidisciplinary Study of Academic Researchers

    ERIC Educational Resources Information Center

    Ge, Xuemei

    2010-01-01

    This article focuses on how electronic information resources influence the information-seeking process in the social sciences and humanities. It examines the information-seeking behavior of scholars in these fields, and extends the David Ellis model of information-seeking behavior for social scientists, which includes six characteristics:…

  4. School Counselor Supervisors' Perceptions of the Discrimination Model of Supervision

    ERIC Educational Resources Information Center

    Luke, Melissa; Ellis, Michael V.; Bernard, Janine M.

    2011-01-01

    The authors examined 38 school counselor supervisors' perceptions of the Discrimination Model (DM; Bernard, 1979, 1997) of supervision, replicating and extending Ellis and Dell's (1986) investigation of the DM with mental health counselor supervisors. Participants judged the dissimilarity of each unique combination of roles and foci of the DM. The…

  5. 39. Photograph of a line drawing. 'SECTIONS SHOWING EQUIPMENT, BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. Photograph of a line drawing. 'SECTIONS SHOWING EQUIPMENT, BUILDING 'G'. Holston Ordnance Works, Tennessee Eastman Corporation. July 25, 1942. Delineator: E. E. Ellis. Drawing # 7651-1007-213. - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

  6. Gauge-invariant cosmic structures---A dynamic systems approach

    SciTech Connect

    Woszczyna, A. )

    1992-03-15

    Gravitational instability is expressed in terms of the dynamic systems theory. The gauge-invariant Ellis-Bruni equation and Bardeen's equation are discussed in detail. It is shown that in an open universe filled with matter of constant sound velocity the Jeans criterion does not adequately define the length scale of the gravitational structure.

  7. The Employment Context. The Impact of the Law on the Lives of Women. Gender and American Law Series, No. 3.

    ERIC Educational Resources Information Center

    Maschke, Karen J., Ed.

    This book contains the following 14 articles on the effects of law on women in the United States: "Protection of Women Workers and the Courts: A Legal Case History" (Ann Corinne Hill); "Sexual Harassment and Race: A Legal Analysis of Discrimination" (Judy Trent Ellis); "Comparable Worth: Is This a Theory for Black Workers?" (Judy Scales-Trent);…

  8. Readings from...Play as a Medium for Learning and Development.

    ERIC Educational Resources Information Center

    Bergen, Doris, Ed.

    The chapters and essays in this book are drawn from the 1988 edition of "Play as a Medium for Learning and Development." The first chapter, "The Challenge of Educational Play," by Bernard Spodek and Olivia N. Saracho, is followed by two essays: (1) "Play and the Origin of Species," (Michael J. Ellis); and (2) "The Struggle between Sacred Play and…

  9. Adaptive Calibration of Children's Physiological Responses to Family Stress: The Utility of Evolutionary Developmental Theory--Comment on Del Giudice et al. (2012) and Sturge-Apple et al. (2012)

    ERIC Educational Resources Information Center

    Bugental, Daphne Blunt

    2012-01-01

    Children's physiological reactions to stress are presented from the broader theoretical perspective of adaptive calibration to the environment, as rooted in life history theory. Del Giudice, Hinnant, Ellis, and El-Sheikh (2012) focus on children's physiological responses to a stressful task as a consequence of their history of family stress.…

  10. Why Is It Difficult to Grasp the Impacts of the Portuguese Quality Assurance System?

    ERIC Educational Resources Information Center

    Veiga, Amélia; Rosa, Maria João; Dias, Diana; Amaral, Alberto

    2013-01-01

    This article analyses the impacts of the Portuguese quality assurance system on academic cultures, using the Cultural Theory proposed by Douglas (1970, 1982) and developed by Thompson, Ellis and Wildavsky (1990) as an explanatory framework for Portuguese academics' preference formation in relation to quality assessment. The Portuguese higher…

  11. An Attempt to Elaborate a Construct to Measure the Degree of Explicitness and Implicitness in ELT Materials

    ERIC Educational Resources Information Center

    Criado Sanchez, Raquel; Perez, Aquilino Sanchez; Gomez, Pascual Cantos

    2010-01-01

    The concepts of "explicit" and "implicit" (knowledge) are at the core of SLA studies. We take "explicit" as conscious and declarative (knowledge); "implicit" as unconscious, automatic and procedural (knowledge) (DeKeyser, 2003; R. Ellis, 2005a, 2005b, 2009; Hulstjin, 2005; Robinson, 1996; Schmidt, 1990, 1994). The importance of those concepts and…

  12. Promises & Challenges. Proceedings of the Annual Conference on Multimedia in Education & Industry (2nd, Savannah, Georgia, July 29-31, 1993).

    ERIC Educational Resources Information Center

    Association for Applied Interactive Multimedia, Columbia, SC.

    This proceedings of the Association for Applied Interactive Multimedia 1993 conference includes the following papers: "Multimedia in Education and Training: 'Promises and Challenges'" (H. D. Ellis); "Critical Thinking in the Multimedia, Self-Paced English Classroom" (L. Mortensen); "Computer Assisted Instruction" (C. Whitehouse); "Cyberteaching:…

  13. Building 810, oblique view to eastsoutheast, 90mm lens. Note buttressed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Building 810, oblique view to east-southeast, 90mm lens. Note buttressed end wall extensions into which main doors nest. - Travis Air Force Base, B-36 Hangar, Between Woodskill Avenue & Ellis, adjacent to Taxiway V & W, Fairfield, Solano County, CA

  14. Detail, building 810, oblique view to northwest of open front ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail, building 810, oblique view to northwest of open front of hangar showing roof truss system, nested doors at left center, 90mm lens. C-5 aircraft in background at right. - Travis Air Force Base, B-36 Hangar, Between Woodskill Avenue & Ellis, adjacent to Taxiway V & W, Fairfield, Solano County, CA

  15. Possibilities and Challenges of Learning German in a Multimodal Environment: A Case Study

    ERIC Educational Resources Information Center

    Abrams, Zsuzsanna Ittzes

    2016-01-01

    Despite a growing body of research on task-based language learning (TBLT) (Samuda & Bygate, 2008; Ellis, 2003), there is still little information available regarding the pedagogical design behind tasks and how they are implemented (Samuda & Bygate, 2008). Scholars in computer-mediated second language (L2) learning have called for research…

  16. 75 FR 25794 - Regulated Navigation Area: Red Bull Air Race World Championship, Upper New York Bay, Lower Hudson...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-10

    ..., 2008, issue of the Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA08 Regulated Navigation Area: Red Bull Air Race World... State Park, New Jersey and Ellis Island, New Jersey and New York for the Red Bull Air Race...

  17. Social Networking and the School Adjustment of Karen Refugee Youth from Burma: Determining the Effects of Ethnic Identity, Bonding Social Capital, and Facebook Use

    ERIC Educational Resources Information Center

    Lewis, Lucy D.

    2013-01-01

    In 2011 alone, over 56,000 refugees were admitted to the United States and a third of these individuals were under the age of 18 (Martin & Yankay, 2012). Researchers have found that the social capital developed through close and confiding relationships is instrumental in the academic outcomes of refugee youth (Kia-Keating & Ellis, 2007;…

  18. A Guide to the Changing Court Rulings on Union Security in the Public Sector: An Introduction.

    ERIC Educational Resources Information Center

    Jascourt, Hugh D.

    1985-01-01

    Introduces two articles that (1) supply the union and management perspectives of the Supreme Court decision in "Ellis vs. Brotherhood of Railway, Airline and Steamship Clerks" and (2) discuss how this decision affects the public sector in the area of education. (MLF)

  19. Understanding Proportional Reasoning in Pre-Service Teachers

    ERIC Educational Resources Information Center

    Johnson, Kim H.

    2013-01-01

    The purpose of this study is to examine the proportional reasoning of pre-service teachers at the beginning of their teacher preparation program using the developmental shifts described by Lobato and Ellis (2010). They cast changes in proportional reasoning as transitions or "shifts" in students' thinking and these shifts can serve as…

  20. Health Instruction Packages: Injections.

    ERIC Educational Resources Information Center

    Dunkleman, Ellie; And Others

    Text, illustrations, and exercises are utilized in this set of four learning modules designed to instruct nursing students in techniques and equipment utilized for intramuscular injections. The first module, "Equipment for Intramuscular Injections" by Ellie Dunkleman, presents guidelines for selecting needles of the proper length and gauge…

  1. Race, Change, and Urban Society. Urban Affairs Annual Reviews, Volume 5.

    ERIC Educational Resources Information Center

    Orleans, Peter, Ed.; Ellis, William Russell, Jr., Ed.

    Contents of this book include 20 chapters: (1) "Race Research: 'Up Against the Wall' in More Ways Than One," W.R. Ellis, Jr. and P. Orleans; (2) "Value Premises in Race Research: The Evolution of Environmentalism," E.L. Paynter; (3) "Power in the Reformulation of Race Research," I. Katznelson; (4) "Caste and Race Conflict in Cross-Cultural…

  2. My Granddaughter

    ERIC Educational Resources Information Center

    Wardle, Francis

    2009-01-01

    In this article, the author describes his granddaughter Elly (Elysia), who is just over a year old. While he deeply enjoys her company as a granddaughter--after all, her favorite activity is to pull his graying beard--as an instructor of child psychology, both for early childhood and psychology students, the author is fascinated with observing…

  3. Urban Ecosystems Research Joins Mainstream Ecology

    EPA Science Inventory

    We appreciate the heightened awareness that Zoë Corbyn’s article brings about the ostensible dearth of urban ecology studies and we laud the efforts of Martin, Ellis, and Blossey to quantify urban research efforts (“Ecologists shun the urban jungle”, online 16 July 2010; http://w...

  4. 76 FR 58262 - American River Power I, LLC; Notice of Preliminary Permit Application Accepted for Filing and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ..., Ohio--Water Power Project No. 14257 to be located at the existing Ellis Ohio Lock Dam No. 11 on the... Energy Regulatory Commission American River Power I, LLC; Notice of Preliminary Permit Application..., 2011, American River Power I, LLC (American River) filed an application for a preliminary...

  5. Grammar Instruction for Adult English Language Learners: A Task-Based Learning Framework

    ERIC Educational Resources Information Center

    Huang, Jiuhan

    2010-01-01

    Over the past few decades, grammar instruction has moved from its central position in traditional language teaching approaches to playing virtually no role in communicative approaches. This article first gives a historical perspective of grammar instruction. Then it outlines the 10 principles of instructed language learning formulated by Ellis and…

  6. 78 FR 10692 - Quarterly Publication of Individuals, Who Have Chosen To Expatriate, as Required by Section 6039G

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-14

    ... ANTHONY BRIGGS THOMAS MARTIN BROWN LAVINA RUTH BUCHANAN ROBERT CHRISTIAN CHEUNG ALLISON CHO MICHAEL KIM DE LAUBESPIN ELEONORE M J M BONAERT DE MAREDSOUS OLIVIER JOHN DESCLEE EISENMEYER HANS MARTIN ELLIS BILLY CAROL... ANDREAS MOSER ALFRED MOSER MARTINA PARGAS DAMIAN ALAN PAULI MADELAINE DORIS PICARD DAVID HENRY PREST...

  7. Parlaying the Prison Experience

    ERIC Educational Resources Information Center

    Gray, Katti

    2010-01-01

    At the close of almost 25 years of winding through New York state's prisons, former Black Panther Eddie Ellis walked away in 1994 with four college degrees he earned while incarcerated and kept treading his singular path as an activist on the issues of police, courts, crime and punishment. He then established the Center for NuLeadership on Urban…

  8. Finding My Voice: [Re]living, [Re]learning, and [Re]searching Becoming a Singer in a Culture of Marginalization

    ERIC Educational Resources Information Center

    Manovski, Miroslav Pavle

    2012-01-01

    Boys who sing in school are often marginalized, taunted, sometimes called "gay." What if "singer" is someone's whole identity, and that person also happens to be gay? This autoethnography (Ellis, 2004) is a story of the education and development of such a singer and how, within this context, a voice teacher with an empowering…

  9. Draft Genome Sequence of the Psychrophilic Gliding Species Gelidibacter algens ACAM 536.

    PubMed

    Bowman, John P

    2016-01-01

    A draft genome sequence was obtained from the type strain of Gelidibacter algens (ACAM 536). This species was isolated from sea-ice diatom assemblages collected from Ellis Fjord, Eastern Antarctica. The genome of ACAM 536 is a single circular chromosome with an estimated size of 4.50 Mbp. PMID:27587822

  10. The Efficacy of Corrective Feedback and Textual Enhancement in Promoting the Acquisition of Grammatical Redundancies

    ERIC Educational Resources Information Center

    Lyddon, Paul A.

    2011-01-01

    Many second language acquisition researchers (e.g., Doughty & Williams, 1998; R. Ellis, 2007; Long, 1996, 2007; Lyster, Lightbown, & Spada, 1999; Russell & Spada, 2006) have advocated the use of negative feedback to promote learner noticing of errors and the internalization of correct forms. At the same time, the true efficacy of this practice is…

  11. Measuring University-Level L2 Learners' Implicit and Explicit Linguistic Knowledge

    ERIC Educational Resources Information Center

    Zhang, Runhan

    2015-01-01

    Although many theoretical issues revolving around implicit and explicit knowledge in second language (L2) acquisition hinge on the ability to measure these two types of knowledge, few empirical studies have attempted to do so. However, R. Ellis (2005) did develop a battery of tests intended to provide relatively separate measures. This study aims…

  12. Second Language Research Forum Colloquia 2009: Colloquium--Measuring the Effectiveness of Focus on Form

    ERIC Educational Resources Information Center

    Loewen, Shawn

    2011-01-01

    Focus on form, i.e. brief attention to language items within a larger meaning-focused context (Long 1991; Ellis 2001), occurs in a variety of L2 instructional contexts. Meta-analyses of the effectiveness of focus on form have found overall positive effects; however, these meta-analyses have commented on the small number of studies available for…

  13. [Dose of tissue tolerance, dose of tumour sterilisation in continuous and discontinuous irradiation (author's transl)].

    PubMed

    Swyngedauw, J

    1975-05-01

    In the radiotherapy of cancer, two conditions are necessary:-- 1 degree sterilisation of the tumor; 2 degrees safeguard of surrounding tissues. In discontinuous irradiation, sterilisation depends on the administration of a certain tumour-dose within a suitable period or number of sessions. At the rate of 5 sessions weekly, the following dose-number law may be formulated (ELLIS): DT = 2,500.N0,22. Perfect tolerance of the connective tissue is in fact obtained if the total dose is equal or less than: N.S.D..N0,24.T0,11. The nominal standard dose of ELLIS, is a limiting dose of about 1,800 rads. ORTON and ELLIS have published tables which permit one to obtain, without an index of saturation of the supporting tissue by means of a series of double entry tables corresponding each to a frequency of 1, 2, 3, 4, 5 sessions per week. Each table provides the T.D.F. factor in relation to the number of sessions and the elementary dose. The T.D.F. factor is about 100 whatever the periodicity, and whatever the subdivisions of the dose when ELLIS' equation is satisfied, i.e. when one is at the limit of tolerance. Furthermore, the T.D.F. factor of two parts, of treatment under various regimens may be added together. It is sufficient for the sum to be equal to 100 to ensure full tolerance, which eliminates all difficulty of manipulation of a fractionated formula. The conditions of obtention of continuous irradiation do not permit one to separate the doses of sterilisation and tolerance, as was the case with discontinuous irradiation but, generally speaking, both in continuous and discontinuous administration the shorter the period of treatment the more the sterilising dose exceeds the tolerance dose, whereas with very small tumours, one may obtain tumour sterilisation within the limits of tolerance of the supporting tissues. For very large or radio-resistant tumours, one may have to, in order to obtain sterilisation, exceed more or less the perfect tolerance defined by T.D.F. 100 or

  14. [Dose of tissue tolerance, dose of tumour sterilisation in continuous and discontinuous irradiation (author's transl)].

    PubMed

    Swyngedauw, J

    1975-05-01

    In the radiotherapy of cancer, two conditions are necessary:-- 1 degree sterilisation of the tumor; 2 degrees safeguard of surrounding tissues. In discontinuous irradiation, sterilisation depends on the administration of a certain tumour-dose within a suitable period or number of sessions. At the rate of 5 sessions weekly, the following dose-number law may be formulated (ELLIS): DT = 2,500.N0,22. Perfect tolerance of the connective tissue is in fact obtained if the total dose is equal or less than: N.S.D..N0,24.T0,11. The nominal standard dose of ELLIS, is a limiting dose of about 1,800 rads. ORTON and ELLIS have published tables which permit one to obtain, without an index of saturation of the supporting tissue by means of a series of double entry tables corresponding each to a frequency of 1, 2, 3, 4, 5 sessions per week. Each table provides the T.D.F. factor in relation to the number of sessions and the elementary dose. The T.D.F. factor is about 100 whatever the periodicity, and whatever the subdivisions of the dose when ELLIS' equation is satisfied, i.e. when one is at the limit of tolerance. Furthermore, the T.D.F. factor of two parts, of treatment under various regimens may be added together. It is sufficient for the sum to be equal to 100 to ensure full tolerance, which eliminates all difficulty of manipulation of a fractionated formula. The conditions of obtention of continuous irradiation do not permit one to separate the doses of sterilisation and tolerance, as was the case with discontinuous irradiation but, generally speaking, both in continuous and discontinuous administration the shorter the period of treatment the more the sterilising dose exceeds the tolerance dose, whereas with very small tumours, one may obtain tumour sterilisation within the limits of tolerance of the supporting tissues. For very large or radio-resistant tumours, one may have to, in order to obtain sterilisation, exceed more or less the perfect tolerance defined by T.D.F. 100 or

  15. Speech-rhythm characteristics of client-centered, Gestalt, and rational-emotive therapy interviews.

    PubMed

    Chen, C L

    1981-07-01

    The aim of this study was to discover whether client-centered, Gestalt, and rational-emotive psychotherapy interviews could be described and differentiated on the basis of quantitative measurement of their speech rhythms. These measures were taken from the sound portion of a film showing interviews by Carl Rogers, Frederick Perls, and Albert Ellis. The variables used were total session and percentage of speaking times, speaking turns, vocalizations, interruptions, inside and switching pauses, and speaking rates. The three types of interview had very distinctive patterns of speech-rhythm variables. These patterns suggested that Rogers's Client-centered therapy interview was patient dominated, that Ellis's rational-emotive therapy interview was therapist dominated, and that Perls's Gestalt therapy interview was neither therapist nor patient dominated.

  16. Study and modeling of the rheological properties of concentrated water-in-oil emulsions

    SciTech Connect

    Koroleva, M.Yu.; Yurtov, E.V.

    1994-07-01

    Study of the rheological curves of concentrated water-in-oil emulsions indicates that such systems behave like non-Newtonian pseudo-plastic liquids. A number of mathematical models for rheological curves: Chong, Frankel-Acrivos, Ostwald-Weil, Bingham, Stainer, Ferry, Haven, Ellis, and Meter models are considered. The regions of the model adequacy for rheological curves of emulsions with different contents of the dispersed phase are determined. It was shown that only the Ellis model adequately describes the complete rheological curves of concentrated water-in-oil emulsions of the studied composition. Therefore, this model can be applied to the prediction of the viscosity values for emulsions with various phase ratios.

  17. Effect of rational and irrational statements on intensity and 'inappropriateness' of emotional distress and irrational beliefs in psychotherapy patients.

    PubMed

    Cramer, D; Kupshik, G

    1993-09-01

    Ellis's rational-emotive theory postulates that since irrational statements augment emotional distress, replacing irrational with rational statements should lessen distress. This hypothesis was tested in the initial stages of psychotherapy by having 13 and 14 clinical out-patients respectively repeat for one minute either rational or irrational statements about their major presenting psychological problem. The distinction by Ellis & Harper (1975) that 'inappropriate' emotions differ qualitatively from 'appropriate' emotions was also examined. Although the experimental intervention had no effect on a post-test measure of irrational beliefs, patients repeating rational statements had significantly lower appropriate and inappropriate negative emotions at post-test, suggesting that inappropriate emotions do not differ qualitatively from appropriate emotions and that making rational statements may lower emotional distress in patients. Patients reiterating irrational statements showed no change in emotions, implying that these kinds of irrational cognitions may have already been present.

  18. Anger and the ABC model underlying Rational-Emotive Behavior Therapy.

    PubMed

    Ziegler, Daniel J; Smith, Phillip N

    2004-06-01

    The ABC model underlying Ellis's Rational-Emotive Behavior Therapy predicts that people who think more irrationally should display greater trait anger than do people who think less irrationally. This study tested this prediction regarding the ABC model. 186 college students were administered the Survey of Personal Beliefs and the State-Trait Anger Expression Inventory-Second Edition to measure irrational thinking and trait anger, respectively. Students who scored higher on Overall Irrational Thinking and Low Frustration Tolerance scored significantly higher on Trait Anger than did those who scored lower on Overall Irrational Thinking and Low Frustration Tolerance. This indicates support for the ABC model, especially Ellis's construct of irrational beliefs which is central to the model.

  19. A test of the ABC model underlying rational emotive behavior therapy.

    PubMed

    Ziegler, Daniel J; Leslie, Yvonne M

    2003-02-01

    The ABC model underlying Ellis's Rational Emotive Behavior Therapy predicts that people who think more irrationally should respond to daily stressors or hassles differently than do people who think less irrationally. This study tested this aspect of the ABC model. 192 college students were administered the Survey of Personal Beliefs and the Hassles Scale to measure irrational thinking and daily hassles, respectively. Students who scored higher on overall irrational thinking reported a significantly higher frequency of hassles than did those who scored lower on overall irrational thinking, while students who scored higher on awfulizing and low frustration tolerance reported a significantly greater intensity of hassles than did those who scored lower on awfulizing and low frustration tolerance. This indicates support for the ABC model, especially Ellis's construct of irrational beliefs central to this model.

  20. Wormhole shadows in rotating dust

    NASA Astrophysics Data System (ADS)

    Ohgami, Takayuki; Sakai, Nobuyuki

    2016-09-01

    As an extension of our previous work, which investigated the shadows of the Ellis wormhole surrounded by nonrotating dust, in this paper we study wormhole shadows in a rotating dust flow. First, we derive steady-state solutions of slowly rotating dust surrounding the wormhole by solving relativistic Euler equations. Solving null geodesic equations and radiation transfer equations, we investigate the images of the wormhole surrounded by dust for the above steady-state solutions. Because the Ellis wormhole spacetime possesses unstable circular orbits of photons, a bright ring appears in the image, just as in Schwarzschild spacetime. The bright ring looks distorted due to rotation. Aside from the bright ring, there appear weakly luminous complex patterns by the emission from the other side of the throat. These structure could be detected by high-resolution very-long-baseline-interferometry observations in the near future.

  1. Physics Textbooks Don't Always Tell the Truth

    NASA Astrophysics Data System (ADS)

    Franklin, Allan

    2016-04-01

    Anyone who studies the history of physics quickly realizes that the history presented in physics textbooks is often inaccurate. I will discuss three episodes from the history of modern physics: (1) Robert Millikan's experiments on the photoelectric effect, (2) the Michelson-Morley experiment, and (3) the Ellis-Wooster experiment on the energy spectrum in β decay. Everyone knows that Millikan's work established the photon theory of light and that the Michelson-Morley experiment was crucial in the genesis of Albert Einstein's special theory of relativity. The problem is that what everyone knows is wrong. Neither experiment played the role assigned to it by physics textbooks. The Ellis-Wooster experiment, on the other hand, is rarely discussed in physics texts, but it should be. It led to Wolfgang Pauli's suggestion of the neutrino. I will present a more accurate history of these three experiments than those given in physics texts.

  2. Intensionality in the irrational beliefs-intellectual performance relationship.

    PubMed

    Prola, M

    1988-01-01

    This study tested the hypothesis that certain semantic processes may be responsible for the previously observed inverse relationship between the endorsement of Ellis's irrational beliefs and measures of intellectual performance. The IS of Identity Scale, a measure of intensional thinking, was administered to 134 male and female entering college freshmen, along with a list of Ellis's irrational beliefs, the Developmental Test of Reading Skills, and locally constructed tests of writing and mathematics. A multiple-regression analysis showed that irrationality uniquely accounted for 13% of reading variance (p less than .001) and none of the variance in the other variables. Intensionality was not associated uniquely with any of the variables and seemingly played no part in the irrationality-intellectual performance relationship.

  3. New distribution record for the rare limpet Acroloxus coloradensis (Henderson) (Gastropoda: Acroloxidae) from Montana

    USGS Publications Warehouse

    Hossack, Blake R.; Robert L. Newell,

    2013-01-01

    The Rocky Mountain Capshell, Acroloxus coloradensis (Henderson, 1930), the only North American member of the basommatophoran family Acroloxidae, is broadly distributed across southern Canada and south into the Rocky Mountains in the USA (Turgeon et al., 1998; Lee and Ackerman, 2000). Despite its wide geographic range, A. coloradensis has been documented from < 30 locations, mostly in British Columbia, Alberta, Ontario, and Quebec (Lee and Ackerman, 2000; Anderson, 2005). Relict populations of A. coloradensis in the USA have been documented from only 6 sites in Colorado and 2 sites in Glacier National Park (Glacier NP), Montana (Anderson, 2005; Ellis et al., 2004). In Glacier NP, A. coloradensis was first reported from Lost Lake (Figure 1; Russell and Brunson, 1967). A second population in the park was discovered in Trout Lake in 2001 (Ellis et al., 2004). In both lakes, A. coloradensis was found primarily under rocks and other cover objects.

  4. Abraham Pais Prize for History of Physics: Physics Textbooks Don't Always Tell the Truth

    NASA Astrophysics Data System (ADS)

    Franklin, Allan

    2016-03-01

    Anyone who studies the history of physics quickly realizes that the history of physics presented in physics textbooks is often inaccurate. This is not necessarily a bad thing. The purpose of textbooks is to help students learn physics. An inaccurate history may serve a pedagogical purpose. It may help to explain concepts more clearly than the actual history. I believe, however, that it is important for those of us who teach physics to know the accurate history. In this talk I will discuss two episodes from the history of modern physics, Millikan's experiments on the photoelectric effect and the Ellis-Wooster experiment on the energy spectrum in β decay. Everyone knows that Millikan's work established the photon theory of light. The problem is that what everyone knows is wrong. The Ellis-Wooster experiment, on the other hand, is rarely discussed in physics texts, but it should be. In this talk I will present a more accurate history of these experiments.

  5. Speech-rhythm characteristics of client-centered, Gestalt, and rational-emotive therapy interviews.

    PubMed

    Chen, C L

    1981-07-01

    The aim of this study was to discover whether client-centered, Gestalt, and rational-emotive psychotherapy interviews could be described and differentiated on the basis of quantitative measurement of their speech rhythms. These measures were taken from the sound portion of a film showing interviews by Carl Rogers, Frederick Perls, and Albert Ellis. The variables used were total session and percentage of speaking times, speaking turns, vocalizations, interruptions, inside and switching pauses, and speaking rates. The three types of interview had very distinctive patterns of speech-rhythm variables. These patterns suggested that Rogers's Client-centered therapy interview was patient dominated, that Ellis's rational-emotive therapy interview was therapist dominated, and that Perls's Gestalt therapy interview was neither therapist nor patient dominated. PMID:7263935

  6. Sound Evidence: The Missing Piece of the Jigsaw in Formulaic Language Research

    ERIC Educational Resources Information Center

    Lin, Phoebe M. S.

    2012-01-01

    With the ever increasing number of studies on formulaic language, we are beginning to learn more about the processing of formulaic language (e.g. Ellis et al. 2008; Siyanova et al. 2011), its use in speech (e.g. Aijmer 1996; Wood 2012) and writing (e.g. Hyland 2008a, 2008b) and its application in natural language processing (e.g. Tschichold 2000).…

  7. Interior, building 810, view to west from approximately midhangar. Area ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior, building 810, view to west from approximately mid-hangar. Area of photo encompasses approximately 1/4 of the interior space, with the KC-10 tanker aircraft and the figures beneath it giving an idea of scale, 90mm lens plus electronic flash fill lightening. - Travis Air Force Base, B-36 Hangar, Between Woodskill Avenue & Ellis, adjacent to Taxiway V & W, Fairfield, Solano County, CA

  8. 76 FR 72613 - Standard Instrument Approach Procedures, and Takeoff Minimums and Obstacle Departure Procedures...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-25

    ... Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a... Lincoln 1/8699 10/31/11 VOR/DME RWY 22, Capital. Orig-A 15-Dec-11......... NC Jacksonville..... Albert J Ellis... 1/8737 10/24/11 NDB RWY 5, Amdt 8 15-Dec-11......... GA Athens Athens/Ben Epps.. 1/8738...

  9. Corrigendum to “Robust limits on Lorentz violation from gamma-ray bursts” [Astropart. Phys. 25 (2006) 402

    NASA Astrophysics Data System (ADS)

    Ellis, John; Mavromatos, N. E.; Nanopoulos, D. V.; Sakharov, A. S.; Sarkisyan, E. K. G.

    2008-03-01

    We correct the fitting formula used [J.R. Ellis, N.E. Mavromatos, D.V. Nanopoulos, A.S. Sakharov, E.K.G. Sarkisyan, Astropart. Phys. 25 (2006) 402. Available from: arxiv:] to obtain a robust limit on a violation of Lorentz invariance that depends linearly on the photon energy. The correction leads to a slight increase of the limit on the scale of the violation, to M > 1.4 ×1016GeV .

  10. Touch receptor of venous flytrap, Dionaea muscipula.

    PubMed

    DiPalma, J R; McMichael, R; DiPalma, M

    1966-04-22

    Numerous small structures (stellate trichomnes) protrude from the surface of marginal hairs, outer leaf surface, and stem of Dionaea muscipula Ellis. None are present inside the trap. Mechanical stimulation causes small action potentials and eventual closure of the trap, independently of the sensitive trigger hairs. Subthreshold stimulation of these structures appears to sensitize the trigger hairs and facilitate closure of the trap. This suiggests that these structures act as touch sensors or receptors.

  11. A muscle contracting substance from a plant's closing Fly-Trap.

    PubMed

    Lea, H W

    1976-01-01

    A muscle contracting substance (MCS) occurs in crushed, incubated traps of the insectivorous plant, the Venus Fly-Trap (Dionaea muscipula Ellis). This MCS is provisionally identified as lysophosphatidic acid. More MCS is produced from traps which have been touched than from untouched traps, which may be due to activation of phospholipase D. This enzyme hydrolyses phospholipids of membranes, and could alter the physiological properties of membranes.

  12. Anisotropic Bianchi types VIII and IX locally rotationally symmetric cosmologies

    SciTech Connect

    Assad, M.J.D.; Soares, I.D.

    1983-10-15

    We present a class of exact cosmological solutions of Einstein-Maxwell equations, which are anisotropic and spatially homogeneous of Bianchi types VIII and IX, and class IIIb in the Stewart-Ellis classification of locally rotationally symmetric models. If we take the electromagnetic field equal to zero, a class of Bianchi types VIII/IX spatially homogeneous anisotropic cosmological solutions with perfect fluid is obtained.

  13. The American Mosaic. Eighth Grade Activity. Schools of California Online Resources for Education (SCORE): Connecting California's Classrooms to the World.

    ERIC Educational Resources Information Center

    Boston, Jaime

    Between 1892-1954, over 12 million immigrants passed through Ellis Island (New York) on their way to becoming part of the United States melting pot. Even though the trip was very difficult, people considered the U.S. to be the land of opportunity. What made the U.S. seem so good? This role playing exercise for eighth-grade students states that…

  14. Comment on the Brans Dicke Bianchi Type-Iii Vacuum Solution

    NASA Astrophysics Data System (ADS)

    Lorenz-Petzold, Dieter

    1985-02-01

    The author wishes to point out that the Brans-Dicke-Bianchi type-III vacuum solution recently given by Tiwari and Singh (1984) is not new. Moreover, the solution given has no correct Einstein limit, contrary to what is claimed by these authors. The Ellis-MacCallum vacuum solution in the Einstein case can be obtained from the Brans-Dicke solution first given by Lorenz-Petzold (1984).

  15. High p{sub T} jet production in pp collisions

    SciTech Connect

    Eskola, K.J.; Wang, X.N.

    1995-07-01

    Production rates of large p{sub T} jets in pp collisions at RHIC and LHC energies are studied using the next-to-leading order calculation of S. D. Ellis, Z. Zunszt and D. Soper. The computed inclusive one-jet cross sections are compared against the CERN and Fermilab jet data from p{bar p} and pp collisions. The dependence of the results on the choice of the parton distributions and renormalization/factorization scales is investigated.

  16. Galaxies at High Redshift

    NASA Astrophysics Data System (ADS)

    Pérez-Fournon, I.; Balcells, M.; Moreno-Insertis, F.; Sánchez, F.

    2010-08-01

    Participants; Group photograph; Preface; Acknowledgements; 1. Galaxy formation and evolution: recent progress R. Ellis; 2. Galaxies at high redshift M. Dickinson; 3. High-redshift galaxies: the far-infrared and sub-millimeter view A. Franceschini; 4. Quasar absorption lines J. Bechtold; 5. Stellar population synthesis models at low and high redshift G. Bruzual A.; 6. Elliptical galaxies K. C. Freeman; 7. Disk galaxies K. C. Freeman; 8. Dark matter in disk galaxies K. C. Freeman.

  17. 10. POWELL STREET RECONSTRUCTION: Photocopy of October 1971 photograph showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. POWELL STREET RECONSTRUCTION: Photocopy of October 1971 photograph showing a Powell-type yoke in place in the street. Photo taken on Powell Street near Ellis during repair work on the conduit. Note brick work of original 1887 conduit walls, and position of the slot rails, track rails, and cable in relationship to the yokes. - San Francisco Cable Railway, Washington & Mason Streets, San Francisco, San Francisco County, CA

  18. Scalar and spinor fields in the very early universe.

    NASA Astrophysics Data System (ADS)

    Srivastava, Sushil K.

    1987-06-01

    Here it is shown how the vacuum energy may dominate the energy density of the very early universe even when the Higg's field in the Coleman-Weinberg potential is confined near the origin at extremely high temperature and the inflationary scenario may start. Also it is shown that supersymmetry breaking may be responsible for this phenomenon. Thus it provides another support for the hypothesis of primordial inflation proposed by Ellis et al. [4],

  19. Investigation on dip coating process by mathematical modeling of non-Newtonian fluid coating on cylindrical substrate

    NASA Astrophysics Data System (ADS)

    Javidi, Mahyar; Pope, Michael A.; Hrymak, Andrew N.

    2016-06-01

    A mathematical model for the dip coating process has been developed for cylindrical geometries with non-Newtonian fluids. This investigation explores the effects of the substrate radius and hydrodynamic behavior of the non-Newtonian viscous fluid on the resulting thin film on the substrate. The coating fluid studied, Dymax 1186-MT, is a resin for fiber optics and used as a matrix to suspend 1 vol. % titanium dioxide particles. The coating substrate is a 100 μm diameter fiber optic diffuser. Ellis viscosity model is applied as a non-Newtonian viscous model for coating thickness prediction, including the influence of viscosity in low shear rates that occurs near the surface of the withdrawal film. In addition, the results of the Newtonian and power law models are compared with the Ellis model outcomes. The rheological properties and surface tension of fluids were analyzed and applied in the models and a good agreement between experimental and analytical solutions was obtained for Ellis model.

  20. Cognitions as determinants of (mal)adaptive emotions and emotionally intelligent behavior in an organizational context.

    PubMed

    Spörrle, Matthias; Welpe, Isabell M; Försterling, Friedrich

    2006-01-01

    This study applies the theoretical concepts of Rational Emotive Behavior Therapy (REBT; Ellis, 1962, 1994) to the analysis of functional and dysfunctional behaviour and emotions in the workplace and tests central assumptions of REBT in an organizational setting. We argue that Ellis' appraisal theory of emotion sheds light on some of the cognitive and emotional antecedents of emotional intelligence and emotionally intelligent behaviour. In an extension of REBT, we posit that adaptive emotions resulting from rational cognitions reflect more emotional intelligence than maladaptive emotions which result from irrational cognitions, because the former lead to functional behaviour. We hypothesize that semantically similar emotions (e.g. annoyance and rage) lead to different behavioural reactions and have a different functionality in an organizational context. The results of scenario experiments using organizational vignettes confirm the central assumptions of Ellis' appraisal theory and support our hypotheses of a correspondence between adaptive emotions and emotionally intelligent behaviour. Additionally, we find evidence that irrational job-related attitudes result in reduced work (but not life) satisfaction.