Skin temperature and core-peripheral temperature gradient as markers of hemodynamic status in critically ill patients: a review.

PubMed

Schey, Bernadette M; Williams, David Y; Bucknall, Tracey

2010-01-01

To examine the evidential basis underpinning the monitoring of skin temperature and core-peripheral temperature gradient as elements of hemodynamic assessment in critically ill and adult cardiac surgical patients. Twenty-six studies examining the efficacy of skin temperature or temperature gradient as markers of hemodynamic status were selected as part of an integrative review. Evidence pertaining to the efficacy of these parameters as markers of cardiac function is equivocal and has not been well appraised in the adult cardiac surgical population. Skin temperature and systemic vascular resistance are also affected by factors other than cardiac output. Skin temperature and core-peripheral temperature gradient should not be considered in isolation from other hemodynamic parameters when assessing cardiac status until they are validated by further large-scale prospective studies. 2010. Published by Mosby, Inc.

On the Resolvability of Steam Assisted Gravity Drainage Reservoirs Using Time-Lapse Gravity Gradiometry

NASA Astrophysics Data System (ADS)

Elliott, E. Judith; Braun, Alexander

2017-11-01

Unconventional heavy oil resource plays are important contributors to oil and gas production, as well as controversial for posing environmental hazards. Monitoring those reservoirs before, during, and after operations would assist both the optimization of economic benefits and the mitigation of potential environmental hazards. This study investigates how gravity gradiometry using superconducting gravimeters could resolve depletion areas in steam assisted gravity drainage (SAGD) reservoirs. This is achieved through modelling of a SAGD reservoir at 1.25 and 5 years of operation. Specifically, the density change structure identified from geological, petrological, and seismic observations is forward modelled for gravity and gradients. Three main parameters have an impact on the resolvability of bitumen depletion volumes and are varied through a suitable parameter space: well pair separation, depth to the well pairs, and survey grid sampling. The results include a resolvability matrix, which identifies reservoirs that could benefit from time-lapse gravity gradiometry monitoring. After 1.25 years of operation, during the rising phase, the resolvable maximum reservoir depth ranges between the surface and 230 m, considering a well pair separation between 80 and 200 m. After 5 years of production, during the spreading phase, the resolvability of depletion volumes around single well pairs is greatly compromised as the depletion volume is closer to the surface, which translates to a larger portion of the gravity signal. The modelled resolvability matrices were derived from visual inspection and spectral analysis of the gravity gradient signatures and can be used to assess the applicability of time-lapse gradiometry to monitor reservoir density changes.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ross, J; Walt Kubilius, W; Thomas Kmetz, T

Resource Conservation and Recovery Act (RCRA) requirements for hazardous waste facilities include 30 years of post-closure monitoring. The use of an objective-based monitoring strategy allows for a significant reduction in the amount of groundwater monitoring required, as the groundwater remediation transitions from an active biosparging system to monitored natural attenuation. The lifecycle of groundwater activities at the landfill has progressed from detection monitoring and plume characterization, to active groundwater remediation, and now to monitored natural attenuation and postclosure monitoring. Thus, the objectives of the groundwater monitoring have changed accordingly. Characterization monitoring evaluated what biogeochemical natural attenuation processes were occurring andmore » determined that elevated levels of radium were naturally occurring. Process monitoring of the biosparging system required comprehensive sampling network up- and down-gradient of the horizontal wells to verify its effectiveness. Currently, the scope of monitoring and reporting can be significantly reduced as the objective is to demonstrate that the alternate concentration limits (ACL) are being met at the point of compliance wells and the maximum contaminant level (MCL) is being met at the surface water point of exposure. The proposed reduction is estimated to save about $2M over the course of the remaining 25 years of postclosure monitoring.« less

What are the driving forces for water lifting in the xylem conduit?

PubMed

Zimmermann, Ulrich; Schneider, Heike; Wegner, Lars H; Wagner, Hans-Jürgen; Szimtenings, Michael; Haase, Axel; Bentrup, Friedrich-Wilhelm

2002-03-01

After Renner had shown convincingly in 1925 that the transpirational water loss generates tensions larger than 0.1 MPa (i.e. negative pressures) in the xylem of cut leafy twigs the Cohesion Theory proposed by Böhm, Askenasy, Dixon and Joly at the end of the 19th century was immediately accepted by plant physiologists. Introduction of the pressure chamber technique by Scholander et al. in 1965 enforced the general belief that tension is the only driving force for water lifting although substantial criticism regarding the technique and/or the Cohesion Theory was published by several authors. As typical for scientific disciplines, the advent of minimal- and non-invasive techniques in the last decade as well as the development of a new, reliable method for xylem sap sampling have challenged this view. Today, xylem pressure gradients, potentials, ion concentrations and volume flows as well as cell turgor pressure gradients can be monitored online in intact transpiring higher plants, and within a given physiological context by using the pressure probe technique and high-resolution NMR imaging techniques, respectively. Application of the pressure probe technique to transpiring plants has shown that negative absolute pressures (down to - 0.6 MPa) and pressure gradients can exist temporarily in the xylem conduit, but that the magnitude and (occasionally) direction of gradients contrasts frequently the belief that tension is the only driving force. This seems to be particularly the case for plants faced with problems of height, drought, freezing and salinity as well as with cavitation of the tensile water. Reviewing the current data base shows that other forces come into operation when exclusively tension fails to lift water against gravity due to environmental conditions. Possible candidates are longitudinal cellular and xylem osmotic pressure gradients, axial potential gradients in the vessels as well as gel- and gas bubble-supported interfacial gradients. The multiforce theory overcomes the problem of the Cohesion Theory that life on earth depends on water being in a highly metastable state.

Dynamics of reactive microbial hotspots in concentration gradients

NASA Astrophysics Data System (ADS)

Hubert, Antoine; Farasin, Julien; Tabuteau, Hervé; Méheust, Yves; Le Borgne, Tanguy

2017-04-01

In subsurface environments, bacteria play a major role in controlling the kinetics of a broad range of biogeochemical reactions. In such environments, nutrients fluxes and solute concentrations needed for bacteria metabolism may be highly variable in space and intermittent in time. This can lead to the formation of reactive hotspots where and when conditions are favorable to particular microorganisms, hence inducing biogeochemical reaction kinetics that differ significantly from those measured in homogeneous model environments. To investigate the impact of chemical gradients on the spatial structure and temporal dynamics of subsurface microorganism populations, we develop microfluidic cells allowing for a precise control of flow and chemical gradient conditions, as well as a quantitative monitoring of the bacteria's spatial distribution and biofilm development. Using the non-motile Escherichia coli JW1908-1 strain and Gallionella as model organisms, we investigate the behavior and development of bacteria over a range of single and double concentration gradients in the concentrations of nutrients, electron donors and electron acceptors. To quantify bacterial activity we use Fluorescein Diacetate (FDA) hydrolysis by bacterial enzymes which transforms FDA into Fluorescein, whose local concentration is measured optically. We thus measure bacterial activity locally from the time derivative of the measured fluorescence. This approach allows time-resolved monitoring of the location and intensity of reactive hotspots in micromodels as a function of the flow and chemical gradient conditions. We discuss consequences for the formation and temporal dynamics of biofilms in the subsurface.

Hydrogeology of shallow basin-fill deposits in areas of Salt Lake Valley, Salt Lake County, Utah

USGS Publications Warehouse

Thiros, Susan A.

2003-01-01

A study of recently developed residential/commercial areas of Salt Lake Valley, Utah, was done from 1999 to 2001 in areas in which shallow ground water has the potential to move to a deeper aquifer that is used for public supply. Thirty monitoring wells were drilled and sampled in 1999 as part of the study. The ground water was either under unconfined or confined conditions, depending on depth to water and the presence or absence of fine-grained deposits. The wells were completed in the shallowest water-bearing zone capable of supplying water. Monitoring-well depths range from 23 to 154 feet. Lithologic, geophysical, hydraulic-conductivity, transmissivity, water-level, and water-temperature data were obtained for or collected from the wells.Silt and clay layers noted on lithologic logs correlate with increases in electrical conductivity and natural gamma radiation shown on many of the electromagnetic-induction and natural gamma logs. Relatively large increases in electrical conductivity, determined from the electromagnetic-induction logs, with no major changes in natural gamma radiation are likely caused by increased dissolved-solids content in the ground water. Some intervals with high electrical conductivity correspond to areas in which water was present during drilling.Unconfined conditions were present at 7 of 20 monitoring wells on the west side and at 2 of 10 wells on the east side of Salt Lake Valley. Fine-grained deposits confine the ground water. Anthropogenic compounds were detected in water sampled from most of the wells, indicating a connection with the land surface. Data were collected from 20 of the monitoring wells to estimate the hydraulic conductivity and transmissivity of the shallow ground-water system. Hydraulic-conductivity values of the shallow aquifer ranged from 30 to 540 feet per day. Transmissivity values of the shallow aquifer ranged from 3 to 1,070 feet squared per day. There is a close linear relation between transmissivity determined from slug-test analysis and transmissivity estimated from specific capacity.Water-level fluctuations were measured in the 30 monitoring wells from 1999 to July 2001. Generally, water-level changes measured in wells on the west side of the valley followed a seasonal trend and wells on the east side showed less fluctuation or a gradual decline during the 2-year period. This may indicate that a larger percentage of recharge to the shallow ground-water system on the west side is from somewhat consistent seasonal sources, such as canals and unconsumed irrigation water, as compared to sources on the east side. Water levels measured in monitoring wells completed in the shallow ground-water system near large-capacity public-supply wells varied in response to ground-water withdrawals from the deeper confined aquifer. Water temperature was monitored in 23 wells. Generally, little or no change in water temperature was measured in monitoring wells with a depth to water greater than about 40 feet. The shallower the water level in the well, the greater the water-temperature change measured during the study.Comparison of water levels measured in the monitoring wells and deeper wells in the same area indicate a downward gradient on the east side of the valley. Water levels in the shallow and deeper aquifers in the secondary recharge area on the west side of the valley were similar to those on the east side. Water levels measured in the monitoring wells and nearby wells completed in the deeper aquifer indicate that the vertical gradient can change with time and stresses on the system.

Temperature distribution by the effect of groundwater flow in an aquifer thermal energy storage system model

NASA Astrophysics Data System (ADS)

Shim, B.

2005-12-01

Aquifer thermal energy storage (ATES) can be a cost-effective and renewable energy source, depending on site-specific thermohydraulic conditions. To design an effective ATES system, the understanding of thermohydraulic processes is necessary. The heat transfer phenomena of an aquifer heat storage system are simulated with the scenario of heat pump operation of pumping and waste water reinjection in a two layered confined aquifer model having the effect of groundwater movement. Temperature distribution of the aquifer model is generated, and hydraulic heads and temperature variations are monitored at both wells during simulation days. The average groundwater velocities are determined with two assumed hydraulic gradients set by boundary conditions, and the effect of groundwater flow are shown at the generated thermal distributions at three different depth slices. The generated temperature contour lines at the hydraulic gradient of 0.001 are shaped circular, and the center is moved less than 5 m to the east in 365 days. However at the hydraulic gradient of 0.01, the contour centers of the east well at each depth slice are moved near the east boundary and the movement of temperature distribution is increased at the lower aquifer. By the analysis of thermal interference data between two wells the efficiency of a heat pump operation model is validated, and the variation of heads is monitored at injection, pumping and stabilized state. The thermal efficiency of the ATES system model is represented as highly depended on groundwater flow velocity and direction. Therefore the hydrogeologic condition for the system site should be carefully surveyed.

Wilson Corners SWMU 001 2014 Annual Long Term Monitoring Report Kennedy Space Center, Florida

NASA Technical Reports Server (NTRS)

Langenbach, James

2015-01-01

This document presents the findings of the 2014 Long Term Monitoring (LTM) that was completed at the Wilson Corners site, located at the National Aeronautics and Space Administration (NASA) John F. Kennedy Space Center (KSC), Florida. The goals of the 2014 annual LTM event were to evaluate the groundwater flow direction and gradient and to monitor the vertical and downgradient horizontal extent of the volatile organic compounds (VOCs) in groundwater at the site. The LTM activities consisted of an annual groundwater sampling event in December 2014, which included the collection of water levels from the LTM wells. During the annual groundwater sampling event, depth to groundwater was measured and VOC samples were collected using passive diffusion bags (PDBs) from 30 monitoring wells. In addition to the LTM sampling, additional assessment sampling was performed at the site using low-flow techniques based on previous LTM results and assessment activities. Assessment of monitoring well MW0052DD was performed by collecting VOC samples using low-flow techniques before and after purging 100 gallons from the well. Monitoring well MW0064 was sampled to supplement shallow VOC data north of Hot Spot 2 and east of Hot Spot 4. Monitoring well MW0089 was sampled due to its proximity to MW0090. MW0090 is screened in a deeper interval and had an unexpected detection of trichloroethene (TCE) during the 2013 LTM, which was corroborated during the March 2014 verification sampling. Monitoring well MW0130 was sampled to provide additional VOC data beneath the semi-confining clay layer in the Hot Spot 2 area.

Finite element modeling to determine thermal residual strain distribution of bonded composite repairs for structural health monitoring design

NASA Astrophysics Data System (ADS)

Baker, Wayne; Jones, Rhys; Davis, Claire; Galea, Stephen C.

2002-11-01

The economic implication of fleet upgrades, particularly in Australia with military aircraft such as the F-111 and F/A-18, has led to an increasing reliance on composite repair technology to address fatigue and corrosion-affected aircraft components. The increasing use of such repairs has led to a research effort to develop various in-situ health monitoring systems that may be incorporated with a repair. This paper reports on the development of a theoretical methodology that uses finite element analysis (FEA) to model the strain profiles which optical sensors, on or within the patch, will be exposed to under various operational scenarios, including load and disbond. Numerical techniques are then used to predict the fibre Bragg grating (FBG) reflections which occur with these strain profiles. The quality of these reflection are a key consideration when designing FBG based structural health monitoring (SHM) systems. This information can be used to optimise the location of both surface mounted, and embedded sensors, and determine feasibility of SHM system design. Research was conducted into the thermal residual strain (TRS) within the patch. A finite element study revealed the presence of significant thermal residual strain gradients along the surface of the tapered region of the patch. As Bragg gratings are particularly sensitive to strain gradients, (producing a result similar to a chirped grating) the strain gradient on the composite at potential sensor locations both under load, and in the event of disbond was considered. A sufficiently high gradient leads to an altered Bragg reflection. These spurious reflections need to be considered, and theoretically obtained reflections can provide information to allow for load scenarios where the Bragg shift is not a smooth, well defined peak. It can also be shown that embedded fibres offer a higher average thermal residual strain reading, while being subject to a much lower strain gradient. This particularly favors the optical disbond detection system that is being developed. While certification concerns exist with embedding sensors in repairs, this study shows that embedded optical fibre sensors may provide for a health monitoring system with enhanced reliability and sensitivity.

Temperature-dependent MR signals in cortical bone: potential for monitoring temperature changes during high-intensity focused ultrasound treatment in bone.

PubMed

Ramsay, Elizabeth; Mougenot, Charles; Kazem, Mohammad; Laetsch, Theodore W; Chopra, Rajiv

2015-10-01

Because existing magnetic resonance thermometry techniques do not provide temperature information within bone, high-intensity focused ultrasound (HIFU) exposures in bone are monitored using temperature changes in adjacent soft tissues. In this study, the potential to monitor temperature changes in cortical bone using a short TE gradient echo sequence is evaluated. The feasibility of this proposed method was initially evaluated by measuring the temperature dependence of the gradient echo signal during cooling of cortical bone samples implanted with fiber-optic temperature sensors. A subsequent experiment involved heating a cortical bone sample using a clinical MR-HIFU system. A consistent relationship between temperature change and the change in magnitude signal was observed within and between cortical bone samples. For the two-dimensional gradient echo sequence implemented in this study, a least-squares linear fit determined the percentage change in signal to be (0.90 ± 0.01)%/°C. This relationship was used to estimate temperature changes observed in the HIFU experiment and these temperatures agreed well with those measured from an implanted fiber-optic sensor. This method appears capable of displaying changes related to temperature in cortical bone and could improve the safety of MR-HIFU treatments. Further investigations into the sensitivity of the technique in vivo are warranted. © 2014 Wiley Periodicals, Inc.

Pure phase encode magnetic field gradient monitor.

PubMed

Han, Hui; MacGregor, Rodney P; Balcom, Bruce J

2009-12-01

Numerous methods have been developed to measure MRI gradient waveforms and k-space trajectories. The most promising new strategy appears to be magnetic field monitoring with RF microprobes. Multiple RF microprobes may record the magnetic field evolution associated with a wide variety of imaging pulse sequences. The method involves exciting one or more test samples and measuring the time evolution of magnetization through the FIDs. Two critical problems remain. The gradient waveform duration is limited by the sample T(2)*, while the k-space maxima are limited by gradient dephasing. The method presented is based on pure phase encode FIDs and solves the above two problems in addition to permitting high strength gradient measurement. A small doped water phantom (1-3 mm droplet, T(1), T(2), T(2)* < 100 micros) within a microprobe is excited by a series of closely spaced broadband RF pulses each followed by FID single point acquisition. Two trial gradient waveforms have been chosen to illustrate the technique, neither of which could be measured by the conventional RF microprobe measurement. The first is an extended duration gradient waveform while the other illustrates the new method's ability to measure gradient waveforms with large net area and/or high amplitude. The new method is a point monitor with simple implementation and low cost hardware requirements.

Correlation of Electrical Resistance to CMC Stress-Strain and Fracture Behavior Under High Heat-Flux Thermal and Stress Gradients

NASA Technical Reports Server (NTRS)

Appleby, Matthew; Morscher, Gregory; Zhu, Dongming

2015-01-01

Because SiCSiC ceramic matrix composites (CMCs) are under consideration for use as turbine engine hot-section components in extreme environments, it becomes necessary to investigate their performance and damage morphologies under complex loading and environmental conditions. Monitoring of electrical resistance (ER) has been shown as an effective tool for detecting damage accumulation of woven melt-infiltrated SiCSiC CMCs. However, ER change under complicated thermo-mechanical loading is not well understood. In this study a systematic approach is taken to determine the capabilities of ER as a relevant non-destructive evaluation technique for high heat-flux testing, including thermal gradients and localized stress concentrations. Room temperature and high temperature, laser-based tensile tests were conducted in which stress-dependent damage locations were determined using modal acoustic emission (AE) monitoring and compared to full-field strain mapping using digital image correlation (DIC). This information is then compared with the results of in-situ ER monitoring, post-test ER inspection and fractography in order to correlate ER response to convoluted loading conditions and damage evolution.

Using a gradient boosting model to improve the performance of low-cost aerosol monitors in a dense, heterogeneous urban environment

NASA Astrophysics Data System (ADS)

Johnson, Nicholas E.; Bonczak, Bartosz; Kontokosta, Constantine E.

2018-07-01

The increased availability and improved quality of new sensing technologies have catalyzed a growing body of research to evaluate and leverage these tools in order to quantify and describe urban environments. Air quality, in particular, has received greater attention because of the well-established links to serious respiratory illnesses and the unprecedented levels of air pollution in developed and developing countries and cities around the world. Though numerous laboratory and field evaluation studies have begun to explore the use and potential of low-cost air quality monitoring devices, the performance and stability of these tools has not been adequately evaluated in complex urban environments, and further research is needed. In this study, we present the design of a low-cost air quality monitoring platform based on the Shinyei PPD42 aerosol monitor and examine the suitability of the sensor for deployment in a dense heterogeneous urban environment. We assess the sensor's performance during a field calibration campaign from February 7th to March 25th 2017 with a reference instrument in New York City, and present a novel calibration approach using a machine learning method that incorporates publicly available meteorological data in order to improve overall sensor performance. We find that while the PPD42 performs well in relation to the reference instrument using linear regression (R2 = 0.36-0.51), a gradient boosting regression tree model can significantly improve device calibration (R2 = 0.68-0.76). We discuss the sensor's performance and reliability when deployed in a dense, heterogeneous urban environment during a period of significant variation in weather conditions, and important considerations when using machine learning techniques to improve the performance of low-cost air quality monitors.

Non-invasive assessment of pulsatile intracranial pressure with phase-contrast magnetic resonance imaging

PubMed Central

Lindstrøm, Erika Kristina; Vatnehol, Svein Are Sirirud; Mardal, Kent-André; Emblem, Kyrre Eeg; Eide, Per Kristian

2017-01-01

Invasive monitoring of pulsatile intracranial pressure can accurately predict shunt response in patients with idiopathic normal pressure hydrocephalus, but may potentially cause complications such as bleeding and infection. We tested how a proposed surrogate parameter for pulsatile intracranial pressure, the phase-contrast magnetic resonance imaging derived pulse pressure gradient, compared with its invasive counterpart. In 22 patients with suspected idiopathic normal pressure hydrocephalus, preceding invasive intracranial pressure monitoring, and any surgical shunt procedure, we calculated the pulse pressure gradient from phase-contrast magnetic resonance imaging derived cerebrospinal fluid flow velocities obtained at the upper cervical spinal canal using a simplified Navier-Stokes equation. Repeated measurements of the pulse pressure gradient were also undertaken in four healthy controls. Of 17 shunted patients, 16 responded, indicating high proportion of “true” normal pressure hydrocephalus in the patient cohort. However, there was no correlation between the magnetic resonance imaging derived pulse pressure gradient and pulsatile intracranial pressure (R = -.18, P = .43). Pulse pressure gradients were also similar in patients and healthy controls (P = .26), and did not differ between individuals with pulsatile intracranial pressure above or below established thresholds for shunt treatment (P = .97). Assessment of pulse pressure gradient at level C2 was therefore not found feasible to replace invasive monitoring of pulsatile intracranial pressure in selection of patients with idiopathic normal pressure hydrocephalus for surgical shunting. Unlike invasive, overnight monitoring, the pulse pressure gradient from magnetic resonance imaging comprises short-term pressure fluctuations only. Moreover, complexity of cervical cerebrospinal fluid flow and -pulsatility at the upper cervical spinal canal may render the pulse pressure gradient a poor surrogate marker for intracranial pressure pulsations. PMID:29190788

Non-invasive assessment of pulsatile intracranial pressure with phase-contrast magnetic resonance imaging.

PubMed

Ringstad, Geir; Lindstrøm, Erika Kristina; Vatnehol, Svein Are Sirirud; Mardal, Kent-André; Emblem, Kyrre Eeg; Eide, Per Kristian

2017-01-01

Invasive monitoring of pulsatile intracranial pressure can accurately predict shunt response in patients with idiopathic normal pressure hydrocephalus, but may potentially cause complications such as bleeding and infection. We tested how a proposed surrogate parameter for pulsatile intracranial pressure, the phase-contrast magnetic resonance imaging derived pulse pressure gradient, compared with its invasive counterpart. In 22 patients with suspected idiopathic normal pressure hydrocephalus, preceding invasive intracranial pressure monitoring, and any surgical shunt procedure, we calculated the pulse pressure gradient from phase-contrast magnetic resonance imaging derived cerebrospinal fluid flow velocities obtained at the upper cervical spinal canal using a simplified Navier-Stokes equation. Repeated measurements of the pulse pressure gradient were also undertaken in four healthy controls. Of 17 shunted patients, 16 responded, indicating high proportion of "true" normal pressure hydrocephalus in the patient cohort. However, there was no correlation between the magnetic resonance imaging derived pulse pressure gradient and pulsatile intracranial pressure (R = -.18, P = .43). Pulse pressure gradients were also similar in patients and healthy controls (P = .26), and did not differ between individuals with pulsatile intracranial pressure above or below established thresholds for shunt treatment (P = .97). Assessment of pulse pressure gradient at level C2 was therefore not found feasible to replace invasive monitoring of pulsatile intracranial pressure in selection of patients with idiopathic normal pressure hydrocephalus for surgical shunting. Unlike invasive, overnight monitoring, the pulse pressure gradient from magnetic resonance imaging comprises short-term pressure fluctuations only. Moreover, complexity of cervical cerebrospinal fluid flow and -pulsatility at the upper cervical spinal canal may render the pulse pressure gradient a poor surrogate marker for intracranial pressure pulsations.

Atmospheric mercury concentrations observed at ground-based monitoring sites globally distributed in the framework of the GMOS network

NASA Astrophysics Data System (ADS)

Sprovieri, Francesca; Pirrone, Nicola; Bencardino, Mariantonia; D'Amore, Francesco; Carbone, Francesco; Cinnirella, Sergio; Mannarino, Valentino; Landis, Matthew; Ebinghaus, Ralf; Weigelt, Andreas; Brunke, Ernst-Günther; Labuschagne, Casper; Martin, Lynwill; Munthe, John; Wängberg, Ingvar; Artaxo, Paulo; Morais, Fernando; Barbosa, Henrique de Melo Jorge; Brito, Joel; Cairns, Warren; Barbante, Carlo; Diéguez, María del Carmen; Garcia, Patricia Elizabeth; Dommergue, Aurélien; Angot, Helene; Magand, Olivier; Skov, Henrik; Horvat, Milena; Kotnik, Jože; Read, Katie Alana; Mendes Neves, Luis; Gawlik, Bernd Manfred; Sena, Fabrizio; Mashyanov, Nikolay; Obolkin, Vladimir; Wip, Dennis; Feng, Xin Bin; Zhang, Hui; Fu, Xuewu; Ramachandran, Ramesh; Cossa, Daniel; Knoery, Joël; Marusczak, Nicolas; Nerentorp, Michelle; Norstrom, Claus

2016-09-01

Long-term monitoring of data of ambient mercury (Hg) on a global scale to assess its emission, transport, atmospheric chemistry, and deposition processes is vital to understanding the impact of Hg pollution on the environment. The Global Mercury Observation System (GMOS) project was funded by the European Commission (http://www.gmos.eu) and started in November 2010 with the overall goal to develop a coordinated global observing system to monitor Hg on a global scale, including a large network of ground-based monitoring stations, ad hoc periodic oceanographic cruises and measurement flights in the lower and upper troposphere as well as in the lower stratosphere. To date, more than 40 ground-based monitoring sites constitute the global network covering many regions where little to no observational data were available before GMOS. This work presents atmospheric Hg concentrations recorded worldwide in the framework of the GMOS project (2010-2015), analyzing Hg measurement results in terms of temporal trends, seasonality and comparability within the network. Major findings highlighted in this paper include a clear gradient of Hg concentrations between the Northern and Southern hemispheres, confirming that the gradient observed is mostly driven by local and regional sources, which can be anthropogenic, natural or a combination of both.

Remote Sensing of Selected Water-Quality Indicators with the Hyperspectral Imager for the Coastal Ocean (HICO) Sensor

DTIC Science & Technology

2014-01-01

monitoring wind -driven re-suspension events (Chen 2006), a predictive factor for patho- gens such as E. coli (Nevers and Whitman 2005), and a...properties where HICO imagery could be acquired as well as along the major salinity gradients of each estuary (Figure 2). A Sea- Bird 25 CTD (Sea- Bird

Monitoring of the spatio-temporal change in the interplate coupling at northeastern Japan subduction zone based on the spatial gradients of surface velocity field

NASA Astrophysics Data System (ADS)

Iinuma, Takeshi

2018-04-01

A monitoring method to grasp the spatio-temporal change in the interplate coupling in a subduction zone based on the spatial gradients of surface displacement rate fields is proposed. I estimated the spatio-temporal change in the interplate coupling along the plate boundary in northeastern (NE) Japan by applying the proposed method to the surface displacement rates based on global positioning system observations. The gradient of the surface velocities is calculated in each swath configured along the direction normal to the Japan Trench for time windows such as 0.5, 1, 2, 3 and 5 yr being shifted by one week during the period of 1997-2016. The gradient of the horizontal velocities is negative and has a large magnitude when the interplate coupling at the shallow part (less than approximately 50 km in depth) beneath the profile is strong, and the sign of the gradient of the vertical velocity is sensitive to the existence of the coupling at the deep part (greater than approximately 50 km in depth). The trench-parallel variation of the spatial gradients of a displacement rate field clearly corresponds to the trench-parallel variation of the amplitude of the interplate coupling on the plate interface, as well as the rupture areas of previous interplate earthquakes. Temporal changes in the trench-parallel variation of the spatial gradient of the displacement rate correspond to the strengthening or weakening of the interplate coupling. We can monitor the temporal change in the interplate coupling state by calculating the spatial gradients of the surface displacement rate field to some extent without performing inversion analyses with applying certain constraint conditions that sometimes cause over- and/or underestimation at areas of limited spatial resolution far from the observation network. The results of the calculation confirm known interplate events in the NE Japan subduction zone, such as the post-seismic slip of the 2003 M8.0 Tokachi-oki and 2005 M7.2 Miyagi-oki earthquakes and the recovery of the interplate coupling around the rupture area of the 1994 M7.6 Sanriku-Haruka-oki earthquake. The results also indicate the semi-periodic occurrence of slow slip events and the expansion of the area of slow slip events before the 2011 Tohoku-oki earthquake (M9.0) approaching the hypocentre of the Tohoku-oki earthquake.

Hydrogeologic characterization of wells HTH-1, UE18r, UE6e, and HTH-3, Nevada Test Site

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lyles, B.F.; McKay, W.A.; Chapman, J.B.

1991-06-01

Monitoring for the migration of contaminants in groundwater or for the proper design of nuclear test emplacement holes at the Nevada Test Site (NTS) requires proper placement and completion of monitoring wells. This is only possible if the hydrogeologic system is understood in a regional and local context, necessitating data from existing wells and boreholes. Though the NTS Groundwater Characterization Project will be drilling wells, their great expense limits the number of new wells. However, there are many existing boreholes and wells on the NTS which have not been completely evaluated hydrologically. Some of these are incorporated in the Longmore » Term Hydrologic Monitoring Program (LTHMP) of the US Environmental Protection Agency (EPA), others are related to the testing programs. In all cases, additional site investigation in necessary to properly interpret the hydrogeologic data from these wells. Monitoring wells on the NTS are poorly characterized with regard to aquifers penetrated, vertical hydraulic gradients, and vertical variations in water quality. One of the goals of the well validation program was to gain a thorough understanding of the parameters needed to interpret the source and fate potential hazardous and radioactive substances that may be detected in these wells in the future. One of the most critical parameters for monitoring is the knowledge of what aquifer or geologic unit is being sampled when a water sample is collected. Pumped water samples are weighted most heavily to the water quality of the most productive (highest transmissivity) aquifer penetrated by the well.« less

[Intraoperative monitoring in artificial respiration of premature and newborn infants. I. Monitoring of respiratory parameters and alveolar ventilation].

PubMed

Lenz, G; Heipertz, W; Leidig, E; Madee, S

1986-06-01

Monitoring of ventilation serves to ensure adequate alveolar ventilation and arterial oxygenation, and to avoid pulmonary damage due to mechanical ventilation. Basic clinical monitoring, i.e., inspection, auscultation (including precordial or oesophageal stethoscope) and monitoring of heart rate and blood pressure, is mandatory. Mechanical ventilation is monitored by ventilation pressures (peak pressure, plateau pressure and endexpiratory pressure), ventilation volumes (measured at the in/expiratory valve of the respirator and by hot-wire anemometry at the tube connector), ventilation rate, and inspiratory oxygen concentration (FiO2). Alveolar ventilation should be continuously and indirectly recorded by capnometry (pECO2) and by measurement of transcutaneous pCO2 (tcpCO2), whereas oxygenation is determined via measurement of transcutaneous pO2 (tcpO2). Invasive monitoring of gas exchange is essential in prolonged or intrathoracic interventions as well as in neonates with cardiopulmonary problems. paCO2 may be estimated by capillary or venous blood gas analysis; arterial blood gas analysis is required for exact determination of paCO2 as well as arteriocutaneous pCO2 (atcDCO2) and arterio-end-expiratory (aEDCO2) gradients.

Hydrogeologic investigations of the southern Española Basin, NM

NASA Astrophysics Data System (ADS)

Earney, T.; Christensen, D.; Horton, A.; Folsom, M.; Kelley, S.

2017-12-01

For the past 5 years, students participating in the Summer of Applied Geophysical Experience (SAGE) have been studying groundwater conditions in the southern Española Basin, in northern New Mexico, with a goal of developing a better understanding of both regional and local scale geothermal anomalies. A regional geothermal gradient map constructed over the study area indicates that there are two regions with anomalously high geothermal gradients, one associated with the Buckman municipal wellfield (BMWF) and the other in the vicinity of the Cerros del Rio volcanic field. Overproduction at the BMWF was responsible for a 100 meter drop in groundwater levels between 1989 and 2003, leading to a significant amount of land subsidence. Repeat measurements of thermal profiles for several monitoring wells at Buckman reveal incremental warming over a 5 year period from 2013 to 2017. Additionally, land elevation recovery was documented between 2007 and 2010 using InSAR (Interferometric Synthetic Aperture Radar). These observations are inferred to be a response to decreased production rates in nearby wells Buckman 1 and 8. This suggests that the groundwater flow system at Buckman is still in a state of recovery from the years of overproduction. The proximity of the well field to a small mapped fault near Buckman 8 potentially explains why geothermal gradients are anomalously high at the BMWF. Monitoring well SF-2b received special attention in an attempt to explain a localized thermal anomaly between 200 and 230 meters down hole. Explanations including geology (faults and stratigraphy) and interference from nearby production wells do not seem to account for the anomaly. The anomaly is therefore interpreted as the result of a damaged casing. Elevated geothermal anomalies at the Cerros del Rio volcanic field could be explained by structural and textural changes to groundwater flow units that occurred when aligned plug and dikes feeding volcanic vents intruded along faults. A conceptual model illustrates that as basaltic dikes intruded rift fill sediments in the Española Basin, nearby sediments would be altered, thereby reducing pore space and permeability of the aquifers, hindering lateral flow and creating a forced convection system by which warm fluids can migrate towards the surface, thus increasing the geothermal gradient.

Integrated Framework for Assessing Impacts of CO₂ Leakage on Groundwater Quality and Monitoring-Network Efficiency: Case Study at a CO₂ Enhanced Oil Recovery Site.

PubMed

Yang, Changbing; Hovorka, Susan D; Treviño, Ramón H; Delgado-Alonso, Jesus

2015-07-21

This study presents a combined use of site characterization, laboratory experiments, single-well push-pull tests (PPTs), and reactive transport modeling to assess potential impacts of CO2 leakage on groundwater quality and leakage-detection ability of a groundwater monitoring network (GMN) in a potable aquifer at a CO2 enhanced oil recovery (CO2 EOR) site. Site characterization indicates that failures of plugged and abandoned wells are possible CO2 leakage pathways. Groundwater chemistry in the shallow aquifer is dominated mainly by silicate mineral weathering, and no CO2 leakage signals have been detected in the shallow aquifer. Results of the laboratory experiments and the field test show no obvious damage to groundwater chemistry should CO2 leakage occur and further were confirmed with a regional-scale reactive transport model (RSRTM) that was built upon the batch experiments and validated with the single-well PPT. Results of the RSRTM indicate that dissolved CO2 as an indicator for CO2 leakage detection works better than dissolved inorganic carbon, pH, and alkalinity at the CO2 EOR site. The detection ability of a GMN was assessed with monitoring efficiency, depending on various factors, including the natural hydraulic gradient, the leakage rate, the number of monitoring wells, the aquifer heterogeneity, and the time for a CO2 plume traveling to the monitoring well.

Remote Detection of Saline Intrusion in a Coastal Aquifer Using Borehole Measurements of Self-Potential

NASA Astrophysics Data System (ADS)

MacAllister, DJ.; Jackson, M. D.; Butler, A. P.; Vinogradov, J.

2018-03-01

Two years of self-potential (SP) measurements were made in a monitoring borehole in the coastal UK Chalk aquifer. The borehole SP data showed a persistent gradient with depth, and temporal variations with a tidal power spectrum consistent with ocean tides. No gradient with depth was observed at a second coastal monitoring borehole ca. 1 km further inland, and no gradient or tidal power spectrum were observed at an inland site ca. 80 km from the coast. Numerical modeling suggests that the SP gradient recorded in the coastal monitoring borehole is dominated by the exclusion-diffusion potential, which arises from the concentration gradient across a saline front in close proximity to, but not intersecting, the base of the borehole. No such saline front is present at the two other monitoring sites. Modeling further suggests that the ocean tidal SP response in the borehole, measured prior to breakthrough of saline water, is dominated by the exclusion-diffusion potential across the saline front, and that the SP fluctuations are due to the tidal movement of the remote front. The electrokinetic potential, caused by changes in hydraulic head across the tide, is one order of magnitude too small to explain the observed SP data. The results suggest that in coastal aquifers, the exclusion-diffusion potential plays a dominant role in borehole SP when a saline front is nearby. The SP gradient with depth indicates the close proximity of the saline front to the borehole and changes in SP at the borehole reflect changes in the location of the saline front. Thus, SP monitoring can be used to facilitate more proactive management of abstraction and saline intrusion in coastal aquifers.

Spatiotemporal norepinephrine mapping using a high-density CMOS microelectrode array.

PubMed

Wydallis, John B; Feeny, Rachel M; Wilson, William; Kern, Tucker; Chen, Tom; Tobet, Stuart; Reynolds, Melissa M; Henry, Charles S

2015-10-21

A high-density amperometric electrode array containing 8192 individually addressable platinum working electrodes with an integrated potentiostat fabricated using Complementary Metal Oxide Semiconductor (CMOS) processes is reported. The array was designed to enable electrochemical imaging of chemical gradients with high spatiotemporal resolution. Electrodes are arranged over a 2 mm × 2 mm surface area into 64 subarrays consisting of 128 individual Pt working electrodes as well as Pt pseudo-reference and auxiliary electrodes. Amperometric measurements of norepinephrine in tissue culture media were used to demonstrate the ability of the array to measure concentration gradients in complex media. Poly(dimethylsiloxane) microfluidics were incorporated to control the chemical concentrations in time and space, and the electrochemical response at each electrode was monitored to generate electrochemical heat maps, demonstrating the array's imaging capabilities. A temporal resolution of 10 ms can be achieved by simultaneously monitoring a single subarray of 128 electrodes. The entire 2 mm × 2 mm area can be electrochemically imaged in 64 seconds by cycling through all subarrays at a rate of 1 Hz per subarray. Monitoring diffusional transport of norepinephrine is used to demonstrate the spatiotemporal resolution capabilities of the system.

Screening tool to evaluate the vulnerability of down-gradient receptors to groundwater contaminants from uncapped landfills

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baker, Ronald J.; Reilly, Timothy J.; Lopez, Anthony

2015-09-15

Highlights: • A spreadsheet-based risk screening tool for groundwater affected by landfills is presented. • Domenico solute transport equations are used to estimate downgradient contaminant concentrations. • Landfills are categorized as presenting high, moderate or low risks. • Analysis of parameter sensitivity and examples of the method’s application are given. • The method has value to regulators and those considering redeveloping closed landfills. - Abstract: A screening tool for quantifying levels of concern for contaminants detected in monitoring wells on or near landfills to down-gradient receptors (streams, wetlands and residential lots) was developed and evaluated. The tool uses Quick Domenicomore » Multi-scenario (QDM), a spreadsheet implementation of Domenico-based solute transport, to estimate concentrations of contaminants reaching receptors under steady-state conditions from a constant-strength source. Unlike most other available Domenico-based model applications, QDM calculates the time for down-gradient contaminant concentrations to approach steady state and appropriate dispersivity values, and allows for up to fifty simulations on a single spreadsheet. Sensitivity of QDM solutions to critical model parameters was quantified. The screening tool uses QDM results to categorize landfills as having high, moderate and low levels of concern, based on contaminant concentrations reaching receptors relative to regulatory concentrations. The application of this tool was demonstrated by assessing levels of concern (as defined by the New Jersey Pinelands Commission) for thirty closed, uncapped landfills in the New Jersey Pinelands National Reserve, using historic water-quality data from monitoring wells on and near landfills and hydraulic parameters from regional flow models. Twelve of these landfills are categorized as having high levels of concern, indicating a need for further assessment. This tool is not a replacement for conventional numerically-based transport model or other available Domenico-based applications, but is suitable for quickly assessing the level of concern posed by a landfill or other contaminant point source before expensive and lengthy monitoring or remediation measures are taken. In addition to quantifying the level of concern using historic groundwater-monitoring data, the tool allows for archiving model scenarios and adding refinements as new data become available.« less

Lithologic and physicochemical properties and hydraulics of flow in and near the freshwater/saline-water transition zone, San Antonio segment of the Edwards aquifer, south-central Texas, based on water-level and borehole geophysical log data, 1999-2007

USGS Publications Warehouse

Lambert, Rebecca B.; Hunt, Andrew G.; Stanton, Gregory P.; Nyman, Michael B.

2010-01-01

The freshwater zone of the San Antonio segment of the Edwards aquifer in south-central Texas (hereinafter, the Edwards aquifer) is bounded to the south and southeast by a zone of transition from freshwater to saline water (hereinafter, the transition zone). The boundary between the two zones is the freshwater/saline-water interface (hereinafter, the interface), defined as the 1,000-milligrams per liter dissolved solids concentration threshold. This report presents the findings of a study, done by the U.S. Geological Survey in cooperation with the San Antonio Water System, to obtain lithologic properties (rock properties associated with known stratigraphic units) and physicochemical properties (fluid conductivity and temperature) and to analyze the hydraulics of flow in and near the transition zone of the Edwards aquifer on the basis of water-level and borehole geophysical log data collected from 15 monitoring wells in four transects during 1999-2007. No identifiable relation between conductivity values from geophysical logs in monitoring wells in all transects and equivalent freshwater heads in the wells at the times the logs were run is evident; and no identifiable relation between conductivity values and vertical flow in the boreholes concurrent with the times the logs were run is evident. The direction of the lateral equivalent freshwater head gradient and thus the potential lateral flow at the interface in the vicinity of the East Uvalde transect fluctuates between into and out of the freshwater zone, depending on recharge and withdrawals. Whether the prevailing direction on average is into or out of the freshwater zone is not clearly indicated. Equivalent freshwater head data do not indicate a prevailing direction of the lateral gradient at the interface in the vicinity of the Tri-County transect. The prevailing direction on average of the lateral gradient and thus potential lateral flow at the interface in the vicinity of the Kyle transect likely is from the transition zone into the freshwater zone. The hypothesis regarding the vertical gradient at the East Uvalde transect, and thus the potential for vertical flow near an interface conceptualized as a surface sloping upward in the direction of the dip of the stratigraphic units, is that the potential for vertical flow fluctuates between into and out of the freshwater zone, depending on recharge and withdrawals. At the Tri-County transect, a downward gradient on the fresh-water side of the interface and an upward gradient on the saline-water side are evidence of opposing potentials that appear to have stabilized the position of the interface over the range of hydrologic conditions that occurred at the times the logs were run. At the Fish Hatchery transect, an upward gradient on the saline-water side of the interface, coupled with the assumption of a sloping interface, implies a vertical gradient from the transition zone into the freshwater zone. This potential for vertical movement of the interface apparently was opposed by the potential (head) on the freshwater side of the interface that kept the interface relatively stable over the range of hydrologic conditions during which the logs were run. The five flow logs for Kyle transect freshwater well KY1 all indicate upward flow that originates from the Glen Rose Limestone, the uppermost unit of the Trinity aquifer; and one log for well KY2 shows upward flow entering the borehole from the Trinity aquifer. These flow data constitute evidence of the potential for flow from the Trinity aquifer into the Edwards aquifer in the vicinity of the Kyle transect. Subsurface temperature data indicate that flow on average is more active, or vigorous, on the freshwater side of the interface than on the saline-water side. A hydraulic connection between the transition zone and the freshwater zone is indicated by similar patterns in the hydrographs of the 15 transect monitoring wells in and near the transition zone and three county index wel

Networked web-cameras monitor congruent seasonal development of birches with phenological field observations

NASA Astrophysics Data System (ADS)

Peltoniemi, Mikko; Aurela, Mika; Böttcher, Kristin; Kolari, Pasi; Loehr, John; Karhu, Jouni; Kubin, Eero; Linkosalmi, Maiju; Melih Tanis, Cemal; Nadir Arslan, Ali

2017-04-01

Ecosystems' potential to provide services, e.g. to sequester carbon is largely driven by the phenological cycle of vegetation. Timing of phenological events is required for understanding and predicting the influence of climate change on ecosystems and to support various analyses of ecosystem functioning. We established a network of cameras for automated monitoring of phenological activity of vegetation in boreal ecosystems of Finland. Cameras were mounted on 14 sites, each site having 1-3 cameras. In this study, we used cameras at 11 of these sites to investigate how well networked cameras detect phenological development of birches (Betula spp.) along the latitudinal gradient. Birches are interesting focal species for the analyses as they are common throughout Finland. In our cameras they often appear in smaller quantities within dominant species in the images. Here, we tested whether small scattered birch image elements allow reliable extraction of color indices and changes therein. We compared automatically derived phenological dates from these birch image elements to visually determined dates from the same image time series, and to independent observations recorded in the phenological monitoring network from the same region. Automatically extracted season start dates based on the change of green color fraction in the spring corresponded well with the visually interpreted start of season, and field observed budburst dates. During the declining season, red color fraction turned out to be superior over green color based indices in predicting leaf yellowing and fall. The latitudinal gradients derived using automated phenological date extraction corresponded well with gradients based on phenological field observations from the same region. We conclude that already small and scattered birch image elements allow reliable extraction of key phenological dates for birch species. Devising cameras for species specific analyses of phenological timing will be useful for explaining variation of time series of satellite based indices, and it will also benefit models describing ecosystem functioning at species or plant functional type level. With the contribution of the LIFE+ financial instrument of the European Union (LIFE12 ENV/FI/000409 Monimet, http://monimet.fmi.fi)

Implications of observed and simulated ambient flow in monitoring wells.

PubMed

Elci, B A; Molz, F J; Waldrop, W R

2001-01-01

A recent paper by Hutchins and Acree (2000) has called attention to ground water sampling bias due to ambient (natural gradient-induced) flows in monitoring wells. Data collected with borehole flowmeters have shown that such ambient flows are ubiquitous in both confined and unconfined aquifers. Developed herein is a detailed three-dimensional model of flow and transport in the vicinity of a fully penetrating monitoring well. The model was used to simulate a measured ambient flow distribution around a test well in a heterogeneous aquifer at the Savannah River Site (SRS) near Aiken, South Carolina. Simulated ambient flows agreed well with measurements. Natural flow was upward, so water entered the well mainly through high K layers in the lower portion of the aquifer and exited through similar layers in the upper portion. The maximum upward discharge in the well was about 0.28 L/min, which implied an induced exchange of 12 m3/month from the bottom half of the aquifer to the upper half. Tracer transport simulations then illustrated how a contaminant located initially in a lower portion of the aquifer was continuously transported into the upper portion and diluted throughout the entire well by in-flowing water. Even after full purging or micropurging, samples from such a well will yield misleading and ambiguous data concerning solute concentrations, location of a contaminant source, and plume geometry. For all of these reasons, use of long-screened monitoring wells should be phased out, unless an appropriate multilevel sampling device prevents vertical flow.

Hydrologic monitoring of a waste-injection well near Milton, Florida, June 1975 - June 1977

USGS Publications Warehouse

Pascale, Charles A.; Martin, J.B.

1978-01-01

This report presents the hydraulic and chemical data collected from June 1, 1975, when injection began, to June 30, 1977 through a monitoring program at a deep-well waste-injection system at the American Cyanamid Company's plant near Milton, about 12 miles northwest of Pensacola. The injection system consists of a primary injection well, a standby injection well, and two deep monitor wells all completed open hole in the lower limestone of the Floridan aquifer and one shallow-monitor well completed in the upper limestone of the Floridan aquifer. Two of the monitor wells and the standby injection well are used to observe hydraulic and geochemical effects of waste injection in the injection zone at locations 8,180 feet northeast, 1,560 feet south, and 1,025 feet southwest of the primary injection well. The shallow-monitor well, used to observe any effects in the first permeable zone above the 200-foot-thick confining bed, is 28 feet north of the primary injection well. Since injection began in June 1975, 607 million gallons of treated industrial liquid waste with a pH of 4.6 to 6.3 and containing high concentrations of nitrate, organic nitrogen and carbon have been injected into a saline-water-filled limestone aquifer. Wellhead pressure at the injection well in June 1977 average 137 pounds per square inch and the hydraulic pressure gradient was 0.53 pound per square inch per foot of depth to the top of the injection zone. Water levels rose from 36 to 74 feet at the three wells used to monitor the injection zone during the 25-month period. The water level in the shallow-monitor well declined about 8 feet. No changes were detected in the chemical character of water from the shallow-monitor well and deep-monitor well-north. Increases in concentration of bicarbonate and dissolved organic carbon were detected in water from the deep-test monitor well in February 1976 and at the standby injection well in August 1976. In addition to increases in bicarbonate and dissolved organic carbon, sulfate, total organic nitrogen, and total nitrogen concentrations have also increased substantially in samples from these wells. Nitrogen gas concentrations in water samples collected at the three deep-monitor wells ranged from 19 to 176 milligrams per liter, methane from 4.5 to 11.4 milligrams per liter, and carbon dioxide from 7.7 to 44 milligrams per liter. The most probable number of denitrifying bacteria in water samples collected at the three deep-monitor wells ranged from less than 2 colonies to 17 colonies per 100 milliliters. None of the water samples collected in April 1977 at the three deep-monitor wells showed positive concentrations of acetone, ethanol, methanol, or acrylonitrile.

Predicting Seawater Intrusion in Coastal Groundwater Boreholes Using Self-Potential Data

NASA Astrophysics Data System (ADS)

Graham, M.; MacAllister, D. J.; Jackson, M.; Vinogradov, J.; Butler, A. P.

2017-12-01

Many coastal groundwater abstraction wells are under threat from seawater intrusion: this is exacerbated in summer by low water tables and increased abstraction. Existing hydrochemistry or geophysical techniques often fail to predict the timing of intrusion events. We investigate whether the presence and transport of seawater can influence self-potentials (SPs) measured within groundwater boreholes, with the aim of using SP monitoring to provide early warning of saline intrusion. SP data collection: SP data were collected from a coastal groundwater borehole and an inland borehole (> 60 km from the coast) in the Seaford Chalk of southern England. The SP gradient in the inland borehole was approximately 0.05 mV/m, while that in the coastal borehole varied from 0.16-0.26 mV/m throughout the monitoring period. Spectral analysis showed that semi-diurnal fluctuations in the SP gradient were several orders of magnitude higher at the coast than inland, indicating a strong influence from oceanic tides. A characteristic decrease in the gradient, or precursor, was observed in the coastal borehole several days prior to seawater intrusion. Modelling results: Hydrodynamic transport and geoelectric modelling suggest that observed pressure changes (associated with the streaming potential) are insufficient to explain either the magnitude of the coastal SP gradient or the semi-diurnal SP fluctuations. By contrast, a model of the exclusion-diffusion potential closely matches these observations and produces a precursor similar to that observed in the field. Sensitivity analysis suggests that both a sharp saline front and spatial variations in the exclusion efficiency arising from aquifer heterogeneities are necessary to explain the SP gradient observed in the coastal borehole. The presence of the precursor in the model depends also on the presence and depth of fractures near the base of the borehole. Conclusions: Our results indicate that SP monitoring, combined with hydrodynamic transport and geoelectric modelling, holds considerable promise as an early warning device for seawater intrusion. We now aim to refine our understanding of the technique by applying it to a range of aquifer types.

Groundwater biofilm dynamics grown in situ along a nutrient gradient.

PubMed

Williamson, Wendy M; Close, Murray E; Leonard, Margaret M; Webber, Judith B; Lin, Susan

2012-01-01

This paper describes the in situ response of groundwater biofilms in an alluvial gravel aquifer system on the Canterbury Plains, New Zealand. Biofilms were developed on aquifer gravel, encased in fine mesh bags and suspended in protective columns in monitoring wells for at least 20 weeks. Four sites were selected in the same groundwater system where previous analyses indicated a gradient of increasing nitrate down the hydraulic gradient from Sites 1 to 4. Measurements during the current study classified the groundwater as oligotrophic. Biofilm responses to the nutrient gradients were assessed using bioassays, with biomass determined using protein and cellular and nucleic acid staining and biofilm activity using enzyme assays for lipid, carbohydrate, phosphate metabolism, and cell viability. In general, biofilm activity decreased as nitrate levels increased from Sites 1 to 4, with the opposite relationship for carbon and phosphorus concentrations. These results showed that the groundwater system supported biofilm growth and that the upper catchment supported efficient and productive biofilms (high ratio of activity per unit biomass). © 2012, Institute of Environmental Science & Research Ltd (ESR). Ground Water © 2012, National Ground Water Association.

Large scale micro-photometry for high resolution pH-characterization during electro-osmotic pumping and modular micro-swimming

NASA Astrophysics Data System (ADS)

Niu, Ran; Khodorov, Stanislav; Weber, Julian; Reinmüller, Alexander; Palberg, Thomas

2017-11-01

Micro-fluidic pumps as well as artificial micro-swimmers are conveniently realized exploiting phoretic solvent flows based on local gradients of temperature, electrolyte concentration or pH. We here present a facile micro-photometric method for monitoring pH gradients and demonstrate its performance and scope on different experimental situations including an electro-osmotic pump and modular micro-swimmers assembled from ion exchange resin beads and polystyrene colloids. In combination with the present microscope and DSLR camera our method offers a 2 μm spatial resolution at video frame rate over a field of view of 3920 × 2602 μm2. Under optimal conditions we achieve a pH-resolution of 0.05 with about equal contributions from statistical and systematical uncertainties. Our quantitative micro-photometric characterization of pH gradients which develop in time and reach out several mm is anticipated to provide valuable input for reliable modeling and simulations of a large variety of complex flow situations involving pH-gradients including artificial micro-swimmers, microfluidic pumping or even electro-convection.

Monitoring Streambed Scour/Deposition Under Nonideal Temperature Signal and Flood Conditions

NASA Astrophysics Data System (ADS)

DeWeese, Timothy; Tonina, Daniele; Luce, Charles

2017-12-01

Streambed erosion and deposition are fundamental geomorphic processes in riverbeds, and monitoring their evolution is important for ecological system management and in-stream infrastructure stability. Previous research showed proof of concept that analysis of paired temperature signals of stream and pore waters can simultaneously provide monitoring scour and deposition, stream sediment thermal regime, and seepage velocity information. However, it did not address challenges often associated with natural systems, including nonideal temperature variations (low-amplitude, nonsinusoidal signal, and vertical thermal gradients) and natural flooding conditions on monitoring scour and deposition processes over time. Here we addressed this knowledge gap by testing the proposed thermal scour-deposition chain (TSDC) methodology, with laboratory experiments to test the impact of nonideal temperature signals under a range of seepage velocities and with a field application during a pulse flood. Both analyses showed excellent match between surveyed and temperature-derived bed elevation changes even under very low temperature signal amplitudes (less than 1°C), nonideal signal shape (sawtooth shape), and strong and changing vertical thermal gradients (4°C/m). Root-mean-square errors on predicting the change in streambed elevations were comparable with the median grain size of the streambed sediment. Future research should focus on improved techniques for temperature signal phase and amplitude extractions, as well as TSDC applications over long periods spanning entire hydrographs.

Volatile organic compounds at two oil and natural gas production well pads in Colorado and Texas using passive samplers.

PubMed

Eisele, Adam P; Mukerjee, Shaibal; Smith, Luther A; Thoma, Eben D; Whitaker, Donald A; Oliver, Karen D; Wu, Tai; Colon, Maribel; Alston, Lillian; Cousett, Tamira A; Miller, Michael C; Smith, Donald M; Stallings, Casson

2016-04-01

A pilot study was conducted in application of the U.S. Environmental Protection Agency (EPA) Methods 325A/B variant for monitoring volatile organic compounds (VOCs) near two oil and natural gas (ONG) production well pads in the Texas Barnett Shale formation and Colorado Denver-Julesburg Basin (DJB), along with a traffic-dominated site in downtown Denver, CO. As indicated in the EPA method, VOC concentrations were measured for 14-day sampling periods using passive-diffusive tube samplers with Carbopack X sorbent at fenceline perimeter and other locations. VOCs were significantly higher at the DJB well pad versus the Barnett well pad and were likely due to higher production levels at the DJB well pad during the study. Benzene and toluene were significantly higher at the DJB well pad versus downtown Denver. Except for perchloroethylene, VOCs measured at passive sampler locations (PSs) along the perimeter of the Barnett well pad were significantly higher than PSs farther away. At the DJB well pad, most VOC concentrations, except perchloroethylene, were significantly higher prior to operational changes than after these changes were made. Though limited, the results suggest passive samplers are precise (duplicate precision usually ≤10%) and that they can be useful to assess spatial gradients and operational conditions at well pad locations over time-integrated periods. Recently enacted EPA Methods 325A/B use passive-diffusive tube samplers to measure benzene at multiple fenceline locations at petrochemical refineries. This pilot study presents initial data demonstrating the utility of Methods 325A/B for monitoring at ONG facilities. Measurements revealed elevated concentrations reflective of production levels and spatial gradients of VOCs relative to source proximity at the Barnett well pad, as well as operational changes at the DJB well pad. Though limited, these findings indicate that Methods 325A/B can be useful in application to characterize VOCs at well pad boundaries.

Subsurface temperature distribution in a tropical alluvial fan

NASA Astrophysics Data System (ADS)

Chen, Wenfu; Chang, Minhsiang; Chen, Juier; Lu, Wanchung; Huang, Chihc; Wang, Yunshuen

2017-04-01

As a groundwater intensive use country, Taiwan's 1/3 water supplies are derived from groundwater. The major aquifers consist of sand and gravel formed in alluvial fans which border the fronts of central mountains. Thanks to high density of monitoring wells which provide a window to see the details of the subsurface temperature distribution and the thermal regime in an alluvial fan system. Our study area, the Choshui Alluvial Fan, is the largest groundwater basin in Taiwan and, located within an area of 2,000 km2, has a population of over 1.5 million. For this work, we investigated temperature-depth profiles using 70 groundwater monitoring wells during 2000 to 2015. Our results show that the distribution of subsurface temperature is influenced by various factors such as groundwater recharge, groundwater flow field, air temperature and land use. The groundwater recharge zone, hills to the upper fan, contains disturbed and smaller geothermal gradients. The lack of clay layers within the upper fan aquifers and fractures that developed in the hills should cause the convection and mixing of cooler recharge water to groundwater, resulting in smaller geothermal gradients. The groundwater temperatures at a depth to 300 m within the upper fan and hill were approximately only 23-24 °C while the current mean ground surface temperature is approximately 26 °C.

Integrating hydrologic and geophysical data to constrain coastal surficial aquifer processes at multiple spatial and temporal scales

USGS Publications Warehouse

Schultz, Gregory M.; Ruppel, Carolyn; Fulton, Patrick; Hyndman, David W.; Day-Lewis, Frederick D.; Singha, Kamini

2007-01-01

Since 1997, repeated, coincident geophysical surveys and extensive hydrologic studies in shallow monitoring wells have been used to study static and dynamic processes associated with surface water-groundwater interaction at a range of spatial scales at the estuarine and ocean boundaries of an undeveloped, permeable barrier island in the Georgia part of the U.S. South Atlantic Bight. Because geophysical and hydrologic data measure different parameters, at different resolution and precision, and over vastly different spatial scales, reconciling the coincident data or even combining complementary inversion, hydrogeochemcial analyses and well-based groundwater monitoring, and, in some cases, limited vegetation mapping to demonstrate the utility of an integrative, multidisciplinary approach for elucidating groundwater processes at spatial scales (tens to thousands of meters) that are often difficult to capture with traditional hydrologic approaches. The case studies highlight regional aquifer characteristics, varying degrees of lateral saltwater intrusion at estuarine boundaries, complex subsurface salinity gradients at the ocean boundary, and imaging of submarsh groundwater discharge and possible free convection in the pore waters of a clastic marsh. This study also documents the use of geophysical techniques for detecting temporal changes in groundwater salinity regimes under natural (not forced) gradients at intratidal to interannual (1998-200 Southeastern U.S.A. drought) time scales.

A gradient model of vegetation and climate utilizing NOAA satellite imagery. Phase 1: Texas transect

NASA Technical Reports Server (NTRS)

Greegor, D.; Norwine, J. (Principal Investigator)

1981-01-01

A climatological model/variable termed the sponge (a measure of moisture availability based on daily temperature maxima and minima, and precipitation) was tested for potential biogeograhic, ecological, and agro-climatological applications. Results, depicted in tabular and graphic form, suggest that, as generalized climatic index, sponge is particularly appropriate for large-area and global vegetation monitoring. The feasibility of utilizing NOAA/AVHRR data for vegetation classification was investigated and a vegetation gradient model that utilizes sponge and AVHRR data was initiated. Along an east-west Texas gradient, vegetation, sponge, and AVHRR pixel data (channels 1 and 2) were obtained for 12 locations. The normalized difference values for the AVHRR data when plotted against vegetation characteristics (biomass, net productivity, leaf area) and sponge values along the Texas gradient suggest that a multivariate gradient model incorporating AVHRR and sponge data may indeed be useful in global vegetation stratification and monitoring.

Brine delineation and monitoring with electrical resistivity tomography and electromagnetic borehole logging at the Fort Knox well field near West Point, Kentucky

USGS Publications Warehouse

Henderson, Rory; Unthank, Michael D.; Zettwoch, Douglas D.; Lane, John W.

2010-01-01

The potable water system at Fort Knox is threatened by brine contamination from improperly abandoned natural gas exploration wells. The Fort Knox well field is located near the town of West Point, Kentucky, in the flood plain of the Ohio River. At the site, unconsolidated sediments approximately 30 – 40 m thick, overlie shale and porous limestone. Brine is believed to flow vertically from the underlying formations to the unconsolidated aquifer through damaged or leaky well casings under a high hydraulic gradient from the artificially pressurized porous limestone, which is utilized for natural gas storage by a regional energy company. Upon reaching the unconsolidated aquifer, brinecontaminated groundwater enters water supply production wells under the pumping‐induced gradient. As part of the Fort Knox remediation strategy to reduce the impact of brine contamination, electrical resistivity tomography (ERT) and borehole electromagnetic (EM) logs are being collected annually to detect gross changes in subsurface conductivity. The 2009 ERT data show areas of high conductivity on the western (contaminated) side of the site with conductivities more than an order of magnitude higher than on the eastern (uncontaminated) side of the site. The areas of high conductivity are interpreted as brine contamination, consistent with known regions of brine contamination. Conductivities from the EM logs are consistent with the results from the ERT inversions. The EM logs show little change between 2008 and 2009, except for some small changes in the brine distribution in well PZ1. Yearly ERT surveys will be continued to detect new areas of brine contamination and monitor the remediation effort.

Study of Chemotaxis and Cell–Cell Interactions in Cancer with Microfluidic Devices

PubMed Central

Sai, Jiqing; Rogers, Matthew; Hockemeyer, Kathryn; Wikswo, John P.; Richmond, Ann

2017-01-01

Microfluidic devices have very broad applications in biological assays from simple chemotaxis assays to much more complicated 3D bioreactors. In this chapter, we describe the design and methods for performing chemotaxis assays using simple microfluidic chemotaxis chambers. With these devices, using real-time video microscopy we can examine the chemotactic responses of neutrophil-like cells under conditions of varying gradient steepness or flow rate and then utilize software programs to calculate the speed and angles of cell migration as gradient steepness and flow are varied. Considering the shearing force generated on the cells by the constant flow that is required to produce and maintain a stable gradient, the trajectories of the cell migration will reflect the net result of both shear force generated by flow and the chemotactic force resulting from the chemokine gradient. Moreover, the effects of mutations in chemokine receptors or the presence of inhibitors of intracellular signals required for gradient sensing can be evaluated in real time. We also describe a method to monitor intracellular signals required for cells to alter cell polarity in response to an abrupt switch in gradient direction. Lastly, we demonstrate an in vitro method for studying the interactions of human cancer cells with human endothelial cells, fibroblasts, and leukocytes, as well as environmental chemokines and cytokines, using 3D microbioreactors that mimic the in vivo microenvironment. PMID:26921940

Development of lichen response indexes using a regional gradient modeling approach for large-scale monitoring of forests

Treesearch

Susan Will-Wolf; Peter Neitlich

2010-01-01

Development of a regional lichen gradient model from community data is a powerful tool to derive lichen indexes of response to environmental factors for large-scale and long-term monitoring of forest ecosystems. The Forest Inventory and Analysis (FIA) Program of the U.S. Department of Agriculture Forest Service includes lichens in its national inventory of forests of...

Stochastic Models of Polymer Systems

DTIC Science & Technology

2016-01-01

SECURITY CLASSIFICATION OF: The stochastic gradient decent algorithm is the now the "algorithm of choice" for very large machine learning problems...information about the behavior of the algorithm. At the same time, we were also able to formulate various acceleration techniques in precise math terms... gradient decent, REPORT DOCUMENTATION PAGE 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 10. SPONSOR/MONITOR’S ACRONYM(S) ARO 8. PERFORMING

Cosmic ray intensity gradients in the solar system

NASA Technical Reports Server (NTRS)

Mckibben, R. B.

1975-01-01

Recent progress in the determination of cosmic-ray intensity gradients is reviewed. Direct satellite measurements of the integral gradient are described together with various types of indirect measurements, including measurements of the Ar-37/Ar-39 ratio in samples from the Lost City meteorite, studies of anisotropies in neutron-monitor counting rates, and analysis of the sidereal diurnal anisotropy observed at a single point on earth. Nucleonic radial gradients and electron gradients measured by satellites in differential energy windows are discussed, and theoretical studies of the physical processes involved in these gradients are summarized. Observations of intensity gradients in heliographic latitude are reported.

Chromium Isotopic Monitoring of HRC-Stimulated Bio-containment at the 100H Test Site, Hanford, Washington

NASA Astrophysics Data System (ADS)

Christensen, J. N.; Brown, S. T.; Brodie, E. L.; Chakraborty, R.; Conrad, M. E.; Long, P. E.; Faybishenko, B.; Hazen, T. C.

2007-12-01

Hexavalent Cr (Cr(VI)) groundwater contamination is a common problem in the U.S. associated with industrial activity (e.g. electroplating, tanning, paints, anti-corrosion). In the particular case of the Hanford Site, Washington, chromate was used primarily to inhibit corrosion in nuclear reactor cooling systems. During the active operation of the Hanford Site, disposal of waste water bearing chromate, and accidental releases to the vadose zone resulted in significant groundwater contamination with local concentrations near the Columbia river reaching over 1000 ppb Cr(VI). In an effort to test an effective bio-containment strategy for groundwater Cr(VI), a site was selected between the 100D and 100H reactor areas with modest concentrations (~100 ppb Cr(VI) over the past two decades). A slow-release 13C labeled polylactate amendment (HRCTM, Regenesis, Ltd.) was injected into groundwater within a sandy formation to stimulate bacterial activity in order to produce conditions that promote the reduction of dissolved Cr(VI) to insoluble Cr(III) complexes [1]. Since the injection of HRCTM in August 2004, groundwater Cr(VI) concentration has been locally below 1 ppb, and reducing conditions have been maintained to at least the present time. The isotopic composition of Cr can be fractionated during reduction from Cr(VI) to Cr(III) and so has the potential to be used as a monitor of hexavalent Cr reduction [2, 3]. This would provide a direct signature of Cr(VI) reduction, discernable from simple attenuation by dilution. In order to explore the use of Cr isotopic measurements for evaluating processes of Cr(VI) reduction, we have analyzed a series of samples in space and time for Cr isotopic composition (δ53Cr, permil deviation of sample 53Cr/52Cr from that of SRM970). Groundwater samples came from the HRC injection well, from multiple depths of three down-gradient wells, and from an up-gradient well. Samples from down-gradient wells have Cr that is isotopically fractionated relative to samples from the up-gradient well. Taken together, samples from a single sampling campaign yield an apparent fractionation of 2.2 ‰. Cr isotopic measurements of the latest samples (June, 2007) confirm continued reduction of Cr(VI) nearly three years after the introduction of HRCTM, suggesting the long-term effectiveness of this bio-stimulated containment strategy. [1] http://esd.lbl.gov/ERT/hanford100h/ [2] Ellis, AS, Johnson, TM and Bohlen, TD (2002) Science. 295:2060-2062. [3] Johnson, TM and Bohlen, TD (2004) Reviews in Mineralogy and Geochemistry, Vol. 55, p.289-317.

Multi-gradient echo MR thermometry for monitoring of the near-field area during MR-guided high intensity focused ultrasound heating

NASA Astrophysics Data System (ADS)

Lam, Mie K.; de Greef, Martijn; Bouwman, Job G.; Moonen, Chrit T. W.; Viergever, Max A.; Bartels, Lambertus W.

2015-10-01

The multi-gradient echo MR thermometry (MGE MRT) method is proposed to use at the interface of the muscle and fat layers found in the abdominal wall, to monitor MR-HIFU heating. As MGE MRT uses fat as a reference, it is field-drift corrected. Relative temperature maps were reconstructed by subtracting absolute temperature maps. Because the absolute temperature maps are reconstructed of individual scans, MGE MRT provides the flexibility of interleaved mapping of temperature changes between two arbitrary time points. The method’s performance was assessed in an ex vivo water bath experiment. An ex vivo HIFU experiment was performed to show the method’s ability to monitor heating of consecutive HIFU sonications and to estimate cooling time constants, in the presence of field drift. The interleaved use between scans of a clinical protocol was demonstrated in vivo in a patient during a clinical uterine fibroid treatment. The relative temperature measurements were accurate (mean absolute error 0.3 °C) and provided excellent visualization of the heating of consecutive HIFU sonications. Maps were reconstructed of estimated cooling time constants and mean ROI values could be well explained by the applied heating pattern. Heating upon HIFU sonication and subsequent cooling could be observed in the in vivo demonstration.

2011 Annual Industrial Wastewater Reuse Report for the Idaho National Laboratory Site's Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond

DOE Office of Scientific and Technical Information (OSTI.GOV)

David Frederick

2012-02-01

This report describes conditions, as required by the state of Idaho Industrial Wastewater Reuse Permit (LA-000160-01), for the wastewater reuse site at the Idaho National Laboratory Site's Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond from November 1, 2010 through October 31, 2011. The report contains the following information: (1) Facility and system description; (2) Permit required effluent monitoring data and loading rates; (3) Groundwater monitoring data; (4) Status of special compliance conditions; and (5) Discussion of the facility's environmental impacts. During the 2011 reporting year, an estimated 6.99 million gallons of wastewater were discharged to themore » Industrial Waste Ditch and Pond which is well below the permit limit of 13 million gallons per year. Using the dissolved iron data, the concentrations of all permit-required analytes in the samples from the down gradient monitoring wells were below the Ground Water Quality Rule Primary and Secondary Constituent Standards.« less

2010 Annual Industrial Wastewater Reuse Report for the Idaho National Laboratory Site's Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond

DOE Office of Scientific and Technical Information (OSTI.GOV)

David B. Frederick

2011-02-01

This report describes conditions, as required by the state of Idaho Industrial Wastewater Reuse Permit (#LA 000160 01), for the wastewater reuse site at the Idaho National Laboratory Site’s Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond from May 1, 2010 through October 31, 2010. The report contains the following information: • Facility and system description • Permit required effluent monitoring data and loading rates • Groundwater monitoring data • Status of special compliance conditions • Discussion of the facility’s environmental impacts During the 2010 partial reporting year, an estimated 3.646 million gallons of wastewater were dischargedmore » to the Industrial Waste Ditch and Pond which is well below the permit limit of 13 million gallons per year. The concentrations of all permit-required analytes in the samples from the down gradient monitoring wells were below the Ground Water Quality Rule Primary and Secondary Constituent Standards.« less

Hydrogeological investigation at Site 5, Willow Grove Naval Air Station/Joint Reserve Base, Horsham Township, Montgomery County, Pennsylvania

USGS Publications Warehouse

Sloto, Ronald A.

2002-01-01

The U.S. Geological Survey conducted borehole geophysical logging, collected and analyzed water-level data, and sampled sections of a rock core to determine the concentration of volatile organic compounds in the aquifer matrix of the Stockton Formation. Borehole geophysical logs were run in three monitor wells. At well 05MW04I, the vertical gradient was upward at depths above 42 feet below land surface (ft bls), downward between 42 and 82 ft bls, and upward below 82 ft bls. At well 05MW05I, a downward vertical gradient was present. At well 05MW12I, the vertical gradient was downward above 112 ft bls and upward below 112 ft bls.Three water-bearing fractures in a 17-foot long rock core from 23.5 to 40.5 ft bls were identified and sampled. Three samples were analyzed from each water-bearing fracture—at the fracture face, 2 centimeters (cm) below the fracture, and 4 cm below the fracture. Fifteen compounds were detected; however, concentrations of seven compounds were less than 1 microgram per kilogram (mg/kg) when detected. Concentrations of benzene (from 0.39 to 3.3 mg/kg), 1,1-dichloroethene (1,1-DCE) (from 0.15 to 13 mg/kg), 1,1,1-trichloroethane (TCA) (from 0.17 to 22 mg/kg), and trichloroethylene (TCE) (from 0.092 to 9.6 mg/kg) were detected in all samples. The highest concentrations detected were for toluene, which was detected at a concentration of 32 and 86 mg/kg in the samples from unweathered sandstone at 2 and 4 cm below the fracture, respectively. Concentrations generally decreased with distance below the fracture in the mudstone samples. Concentrations of benzene and toluene increased with distance below the fractures in the unweathered sandstone samples. Concentrations of 1,1-DCE, TCA, and TCE were higher in the mudstone samples than in the samples from sandstone. Toluene concentrations were higher in unweathered sandstone than in weathered sandstone or mudstone.The effect of the pumping of Horsham Water and Sewer Authority public supply well 26 (HWSA-26), 0.2 mile southwest of the base boundary, on groundwater levels on the base was determined by shutting the well down for 6 days to allow water levels to recover. Water levels in 22 nearby wells were measured. The only well (02MW01I) that showed an unambiguous response to the shutdown of well HWSA-26 is 1,350 feet directly along strike from well HWSA-26. The recovery of well 05MW11I in response to the shutdown of well HWSA-26 is masked by recharge from snowmelt but probably does not exceed about 0.2 feet on the basis of the water level in well 05MW11I, which showed a response to the pumping of well HWSA-26 that ranged from 0.5 to 0.15 foot.Horizontal gradients differ with depth, and the rate and direction of ground-water flow and contaminant movement is depth dependent. The potentiometric-surface map for water levels measured in wells screened between 5 and 44 ft bls in the aquifer shows a ground-water mound that is the high point on a regional ground-water divide. From this divide, ground water flows both northwest toward Park Creek and southeast toward Pennypack Creek. The hydraulic gradient around this mound is relatively flat to the southeast and particularly flat to the northwest. The potentiometric-surface map for water levels measured in wells screened between 40 and 100 ft bls in the aquifer shows a very flat hydraulic gradient. Differences in the elevation of the potentiometric surface are less than 2 feet. The potentiometric-surface map for water levels measured in wells screened between 105 and 179 ft bls in the aquifer shows a steep hydraulic gradient between Sites 5 and 2 and a relatively flat hydraulic gradient between Sites 5 and 3. Water levels measured on October 7, 1999, showed downward vertical head gradients for all well clusters at Site 5. Vertical gradients ranged from 0.01 at well cluster 05MW10 to 0.2 at cluster 05MW11. Most gradients were between 0.01 and 0.026. Vertical head gradients vary with time. The variability is caused by a difference in the magnitude of water-level fluctuations between shallow and the deep fractures. The difference in the magnitude of water-level fluctuations is because of differences in lithology and aquifer storativity.

Opportunities and challenges for the application of SP measurements to monitor subsurface flow (Invited)

NASA Astrophysics Data System (ADS)

Jackson, M.; Vinogradov, J.; MacAllister, D.; Butler, A. P.; Leinov, E.; Zhang, J.

2013-12-01

Measurements of self-potential (SP) have been proposed or applied to monitor flow in the shallow subsurface in numerous settings, including volcanoes, earthquake zones, geothermal fields and hydrocarbon reservoirs, to detect leaks from dams, tanks and embankments, and to characterize groundwater flow and hydraulic properties. To interpret the measurements, it is generally assumed that the SP is dominated by the streaming potential, arising from the drag of excess electrical charge in the diffuse part of the electrical double layer at the mineral-fluid interfaces. The constitutive equation relating electrical current density j to the driving forces ▽V and ▽P is then j = -σ▽V -σC▽P=-σ▽V + Qv (1) where V is the streaming potential, P is the water pressure, σ is the saturated rock conductivity, v is the Darcy velocity, C is the streaming potential coupling coefficient, and Q is the excess charge transported by the flow. Equation (1) shows that there is a close relationship between flow properties of interest, such as the pressure gradient or Darcy velocity, and the streaming potential component of the SP. Hence SP measurements are an attractive method to monitor subsurface flow. However, the problem with interpreting the measurements is that both C and Q can vary over orders of magnitude, in response to variations in pore-water salinity, temperature, rock texture, and the presence of NAPLs in the pore-space. Moreover, additional current sources may be present if there are gradients in concentration or temperature, arising from differential rates of ion migration down gradient (diffusion potentials), and because of charge exclusion from the pore-space (exclusion potentials). In general, these additional current sources are neglected. This talk suggests a potential new opportunity for the application of SP measurements to monitor subsurface flow, in which the signal of interest arises from salinity rather than pressure gradients. Saline intrusion into freshwater aquifers is a global problem, threatening the water supply of millions of people in coastal settlements. Abstraction rates could be much more efficiently managed if encroaching saline water could be detected before it arrived at the borehole. However, current monitoring is based largely on borehole conductivity measurements, which requires a dense network of monitoring boreholes to map the saline front. Recent laboratory and field experiments suggest that the concentration gradient associated with the front generates an SP signal which can be detected at an abstraction well prior to the arrival of the front, potentially allowing monitoring using a comparatively cheap array of non-polarising borehole electrodes. Current challenges in interpreting SP measurements for subsurface flow are also discussed, particularly the use of models to predict the values of C and Q. The importance of accounting for the pore-level distribution of flow and excess charge in such models is emphasised, and a way forward is suggested in which pore-scale network models, used previously to predict relative permeability and capillary pressure, are extended to include charge transport at the pore-level.

Genetic diversity and population structure in contemporary house sparrow populations along an urbanization gradient

PubMed Central

Vangestel, C; Mergeay, J; Dawson, D A; Callens, T; Vandomme, V; Lens, L

2012-01-01

House sparrow (Passer domesticus) populations have suffered major declines in urban as well as rural areas, while remaining relatively stable in suburban ones. Yet, to date no exhaustive attempt has been made to examine how, and to what extent, spatial variation in population demography is reflected in genetic population structuring along contemporary urbanization gradients. Here we use putatively neutral microsatellite loci to study if and how genetic variation can be partitioned in a hierarchical way among different urbanization classes. Principal coordinate analyses did not support the hypothesis that urban/suburban and rural populations comprise two distinct genetic clusters. Comparison of FST values at different hierarchical scales revealed drift as an important force of population differentiation. Redundancy analyses revealed that genetic structure was strongly affected by both spatial variation and level of urbanization. The results shown here can be used as baseline information for future genetic monitoring programmes and provide additional insights into contemporary house sparrow dynamics along urbanization gradients. PMID:22588131

Genetic diversity and population structure in contemporary house sparrow populations along an urbanization gradient.

PubMed

Vangestel, C; Mergeay, J; Dawson, D A; Callens, T; Vandomme, V; Lens, L

2012-09-01

House sparrow (Passer domesticus) populations have suffered major declines in urban as well as rural areas, while remaining relatively stable in suburban ones. Yet, to date no exhaustive attempt has been made to examine how, and to what extent, spatial variation in population demography is reflected in genetic population structuring along contemporary urbanization gradients. Here we use putatively neutral microsatellite loci to study if and how genetic variation can be partitioned in a hierarchical way among different urbanization classes. Principal coordinate analyses did not support the hypothesis that urban/suburban and rural populations comprise two distinct genetic clusters. Comparison of FST values at different hierarchical scales revealed drift as an important force of population differentiation. Redundancy analyses revealed that genetic structure was strongly affected by both spatial variation and level of urbanization. The results shown here can be used as baseline information for future genetic monitoring programmes and provide additional insights into contemporary house sparrow dynamics along urbanization gradients.

Monitoring gradient profile on-line in micro- and nano-high performance liquid chromatography using conductivity detection.

PubMed

Zhang, Min; Chen, Apeng; Lu, Joann J; Cao, Chengxi; Liu, Shaorong

2016-08-19

In micro- or nano-flow high performance liquid chromatography (HPLC), flow-splitters and gradient elutions are commonly used for reverse phase HPLC separations. When a flow splitter was used at a high split-ratio (e.g., 1000:1 or higher), the actual gradient may deviate away from the programmed gradient. Sometimes, mobile phase concentrations can deviate by as much as 5%. In this work, we noticed that the conductivity (σ) of a gradient decreased with the increasing organic-solvent fraction (φ). Based on the relationship between σ and φ, a method was developed for monitoring gradient profile on-line to record any deviations in these HPLC systems. The conductivity could be measured by a traditional conductivity detector or a capacitively coupled contactless conductivity detector (C(4)D). The method was applied for assessing the performance of an electroosmotic pump (EOP) based nano-HPLC. We also observed that σ value of the gradient changed with system pressure; a=0.0175ΔP (R(2)=0.964), where a is the percentage of the conductivity increase and ΔP is the system pressure in bar. This effect was also investigated. Copyright © 2016. Published by Elsevier B.V.

Se Isotopes as groundwater redox indicators: Detecting natural attenuation of Se at an in situ recovery U mine

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anirban, Basu; Schilling, Kathrin; Brown, Shaun T.

One of the major ecological concerns associated with the in situ recovery (ISR) of uranium (U) is the environmental release of soluble, toxic selenium (Se) oxyanions generated by mining. Post-mining natural attenuation by the residual reductants in the ore body and reduced down-gradient sediments should mitigate the risk of Se contamination in groundwater. Here in this work, we investigate the Se concentrations and Se isotope systematics of groundwater and of U ore bearing sediments from an ISR site at Rosita, TX, USA. Our results show that selenate (Se(VI)) is the dominant Se species in Rosita groundwater, and while several up-gradientmore » wells have elevated Se(VI), the majority of the ore zone and down-gradient wells have little or no Se oxyanions. In addition, the δ 82SeVI of Rosita groundwater is generally elevated relative to the U ore up to +6.14‰, with the most enriched values observed in the ore-zone wells. Increasing δ 82Se with decreasing Se(VI) conforms to a Rayleigh type distillation model with an ε of $-$2.25‰ ± 0.61‰, suggesting natural Se(VI) reduction occurring along the hydraulic gradient at the Rosita ISR site. Moreover, our results show that Se isotopes are excellent sensors for detecting and monitoring post-mining natural attenuation of Se oxyanions at ISR sites.« less

Se Isotopes as groundwater redox indicators: Detecting natural attenuation of Se at an in situ recovery U mine

DOE PAGES

Anirban, Basu; Schilling, Kathrin; Brown, Shaun T.; ...

2016-08-22

One of the major ecological concerns associated with the in situ recovery (ISR) of uranium (U) is the environmental release of soluble, toxic selenium (Se) oxyanions generated by mining. Post-mining natural attenuation by the residual reductants in the ore body and reduced down-gradient sediments should mitigate the risk of Se contamination in groundwater. Here in this work, we investigate the Se concentrations and Se isotope systematics of groundwater and of U ore bearing sediments from an ISR site at Rosita, TX, USA. Our results show that selenate (Se(VI)) is the dominant Se species in Rosita groundwater, and while several up-gradientmore » wells have elevated Se(VI), the majority of the ore zone and down-gradient wells have little or no Se oxyanions. In addition, the δ 82SeVI of Rosita groundwater is generally elevated relative to the U ore up to +6.14‰, with the most enriched values observed in the ore-zone wells. Increasing δ 82Se with decreasing Se(VI) conforms to a Rayleigh type distillation model with an ε of $-$2.25‰ ± 0.61‰, suggesting natural Se(VI) reduction occurring along the hydraulic gradient at the Rosita ISR site. Moreover, our results show that Se isotopes are excellent sensors for detecting and monitoring post-mining natural attenuation of Se oxyanions at ISR sites.« less

Differentiation and Monitoring of Cells Using a Biochip for Regenerative Medicine

NASA Astrophysics Data System (ADS)

Uchida, Tomoyuki; Arai, Fumihito; Suzuki, Osamu; Ichikawa, Akihiko; Fukuda, Toshio; Katagiri, Takenobu; Kamijo, Ryutaro; Nakamura, Masanori; Numata, Mamoru; Watanabe, Naruaki

A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST2 cells) and myoblasts (C2C12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.

Coral reef monitoring in the Iles Eparses, Mozambique Channel (2011-2013)

NASA Astrophysics Data System (ADS)

Chabanet, P.; Bigot, L.; Nicet, J.-B.; Durville, P.; Massé, L.; Mulochau, T.; Russo, C.; Tessier, E.; Obura, D.

2016-04-01

Monitoring of coral reefs has become a major tool for understanding how they are changing, and for managing them in a context of increasing degradation of coastal ecosystems. The Global Coral Reef Monitoring Network (GCRMN) has near-global coverage, but there are few remote sites free of direct human impact that can serve as reference sites. This study provides baseline data for the French Iles Eparses in the Mozambique Channel, Western Indian Ocean (WIO), whose coral reefs are little known owing to their limited accessibility, and have been free from fishing pressure for over 20 years. Surveys of coral reef health and fish community structure were undertaken at four of the islands (Europa, Bassas da India, Juan de Nova and Glorieuses) in 2011-2013. Monitoring was conducted using standardized GCRMN methods for benthos and fish communities, at the highest taxonomic level. Benthic cover showed a latitudinal gradient, with higher coral cover and conversely lower algae cover (60% and 14% respectively) in the south of the Mozambique Channel. This could be due to the geomorphology of the islands, the latitudinal temperature gradient, and/or the history of chronic stress and bleaching events during the last decades. Fish also showed a latitudinal gradient with higher diversity in the north, in a center of diversity for the western Indian Ocean already recognized for corals. An exceptional biomass fish was recorded (approximately 3500 kg/ha excluding sharks, compared to a maximum of 1400 kg/ha elsewhere in the WIO). The presence of large predators and sharks in all the islands as well as the absence of fleshy benthic algae were indicators of the good health of the reef systems. Nevertheless, these islands are beginning to experience illegal fishing, particularly in the north of the Mozambique Channel, demonstrating their vulnerability to exploitation and the need to protect them as reference sites for coral reef studies, including of climate change impacts, for the region and globally.

Is polychaete family-level sufficient to assess impact on tropical estuarine gradients?

NASA Astrophysics Data System (ADS)

Nóbrega-Silva, Climélia; Patrício, Joana; Marques, João Carlos; Olímpio, Monalisa dos Santos; Farias, Jéssica Natyelle Barros; Molozzi, Joseline

2016-11-01

Regular, robust monitoring programs set up to assess the environmental conditions of aquatic systems often target different biological groups. And, of these, macroinvertebrate communities and particularly the class Polychaeta are frequently used. Identifying these organisms takes time, money and specialized expertise to ensure correct identification to the lowest possible taxonomic level. Identification errors can lead to an erroneous assessment. The concept of taxonomic sufficiency has been proposed both to minimize errors and to save time and money. This study tested the usefulness of this concept in tropical estuaries in northeast Brazil. We selected two transitional systems with different degrees of human impact due to different land uses and different conservation systems: the Mamanguape estuary, which is in an environmental conservation unit for sustainable use, and the highly impacted, urban Paraíba do Norte estuary. The results clearly showed that nutrient concentrations were markedly higher in the Paraíba do Norte estuary in the dry season and that the composition of the polychaete assemblages differed between the two estuaries as well as along the spatial gradient of each estuary. The use of either genus or family level led to equivalent representation in each system in terms of taxon richness and both the Margalef and Shannon-Wiener diversity indices. Both taxonomic levels described similar changes in the polychaete assemblage along the estuarine gradients. Based on our findings, the use of a coarser taxonomic level (i.e., family) is a good option when the aim is to implement a monitoring program in tropical estuaries with the polychaete assemblages as one of the target groups. This time-efficient taxonomic resolution can help improve sampling designs and allow long-term monitoring studies without losing much vital information.

Geophysical Logs, Aquifer Tests, and Water Levels in Wells in and Near the North Penn Area 7 Superfund Site, Upper Gwynedd Township, Montgomery County, Pennsylvania, 2002-2006

USGS Publications Warehouse

Senior, Lisa A.; Conger, Randall W.; Bird, Philip H.

2008-01-01

Ground water in the vicinity of several industrial facilities in Upper Gwynedd Township and Lansdale Borough, Montgomery County, Pa., is contaminated with several volatile organic compounds (VOCs). The 2-square-mile area was placed on the National Priorities List as the North Penn Area 7 Superfund Site by the U.S. Environmental Protection Agency (USEPA) in 1989. The U.S. Geological Survey (USGS) conducted geophysical logging, aquifer testing, water-level monitoring, and streamflow measurements in the vicinity of North Penn Area 7 from October 2002 through December 2006. This followed work that began in 2000 to assist the USEPA in developing an understanding of the hydrogeologic framework in the area as part of the USEPA Remedial Investigation. The study area is underlain by Triassic- and Jurassic-age sandstones, siltstones, and shales of the Lockatong Formation and the Brunswick Group. Regionally, these rocks strike northeast and dip to the northwest. The sequence of rocks form fractured-rock aquifers that act as a set of confined to semi-confined layered aquifers of differing permeabilities. The aquifers are recharged by precipitation and discharge to streams and wells. The Wissahickon Creek headwaters are less than 1 mile northeast of the study area. This stream flows southwest approximately parallel to strike and bisects North Penn Area 7. Ground water is pumped in the vicinity of North Penn Area 7 for industrial use and public supply. The USGS collected geophysical logs for 42 wells that ranged in depth from 40 to 477 ft. Aquifer-interval-isolation testing was done in 17 of the 42 wells, for a total of 122 zones tested. A multiple-well aquifer test was conducted by monitoring the response of 14 wells to pumping and shutdown of a 600-ft deep production well in November-December 2004. In addition, water levels were monitored continuously in four wells in the area from October 2002 through September 2006, and streamflow was measured quarterly at two sites on Wissahickon Creek from December 2002 through September 2005. Geophysical logging identified water-bearing zones associated with high-angle fractures and bedding-plane openings throughout the depth of the boreholes. Heatpulse-flowmeter measurements under non-pumping, ambient conditions in 16 wells greater than 200 ft in depth indicated that borehole flow, where detected, was only upward in 2 wells and only downward in 5 wells. In nine wells, both upward and downward flow were measured. Geologic structure and pumping in the area affect the spatial distribution of vertical gradients. Heatpulse-flowmeter measurements under pumping conditions were used to identify the most productive intervals in wells. Correlation of natural-gamma-ray logs indicated bedding in the area probably strikes about 45 to 65 degrees northeast and dips about 9 degrees northwest. Aquifer intervals isolated by inflatable packers in 17 wells were pumped to test productivity of water-bearing zones and to collect samples to determine chemical quality of water produced from the interval. Interval-isolation testing confirmed the vertical hydraulic gradients indicated by heatpulse-flowmeter measurements. The specific capacities of the 122 isolated intervals ranged over about three orders of magnitude, from 0.01 to 10.6 gallons per minute per foot, corresponding to calculated transmissivities of 1.2 to 2,290 feet squared per day. Intervals adjacent to isolated pumped intervals commonly showed little response to pumping of the isolated zone. The presence of vertical hydraulic gradients and lack of adjacent-interval response to pumping in isolated intervals indicate a limited degree of vertical hydraulic connection between the aquifer sections tested. Differences were apparent in inorganic water quality of water from isolated intervals, including pH, specific conductance, and dissolved oxygen. Concentrations of most VOC contaminants in most wells with predominantly upward vertical gradients were g

Wood-inhabiting fungi in southern Italy forest stands: morphogroups, vegetation types and decay classes.

PubMed

Granito, Vito Mario; Lunghini, Dario; Maggi, Oriana; Persiani, Anna Maria

2015-01-01

The authors conducted an ecological study of forests subjected to varying management. The aim of the study was to extend and integrate, within a multivariate context, knowledge of how saproxylic fungal communities behave along altitudinal/vegetational gradients in response to the varying features and quality of coarse woody debris (CWD). The intra-annual seasonal monitoring of saproxylic fungi, based on sporocarp inventories, was used to investigate saproxylic fungi in relation to vegetation types and management categories. We analyzed fungal species occurrence, recorded according to the presence/absence and frequency of sporocarps, on the basis of the harvest season, of coarse woody debris decay classes as well as other environmental and ecological variables. Two-way cluster analysis, DCA and Spearman's rank correlations, for indirect gradient analysis, were performed to identify any patterns of seasonality and decay. Most of the species were found on CWD in an intermediate decay stage. The first DCA axis revealed the vegetational/microclimate gradient as the main driver of fungal community composition, while the second axis corresponded to a strong gradient of CWD decay classes. © 2015 by The Mycological Society of America.

Evaluation of thermometric monitoring for intradiscal laser ablation in an open 1.0 T MR scanner.

PubMed

Wonneberger, Uta; Schnackenburg, Bernhard; Wlodarczyk, Waldemar; Rump, Jens; Walter, Thula; Streitparth, Florian; Teichgräber, Ulf Karl Mart

2010-01-01

The purpose of this study was to evaluate different methods of magnetic resonance thermometry (MRTh) for the monitoring of intradiscal laser ablation therapy in an open 1.0 Tesla magnetic resonance (MR) scanner. MRTh methods based on the two endogenous MR temperature indicators of spin-lattice relaxation time T1 and water proton resonance frequency (PRF) shift were optimised and compared in vitro. For the latter, we measured the effective spin-spin relaxation times T2* in intervertebral discs of volunteers. Then we compared four gradient echo-based imaging techniques to monitor laser ablations in human disc specimens. Criteria of assessment were outline of anatomic detail, immunity against needle artefacts, signal-to-noise ratio (SNR) and accuracy of the calculated temperature. T2* decreased in an inverse and almost linear manner with the patients' age (r = 0.9) from 70 to 30 ms (mean of 49 ms). The optimum image quality (anatomic details, needle artefacts, SNR) and temperature accuracy (+/-1.09 degrees C for T1-based and +/-1.11 degrees C for PRF-based MRTh) was achieved with a non-spoiled gradient-echo sequence with an echo time of TE = 10 ms. Combination of anatomic and thermometric non-invasive monitoring of laser ablations in the lumbar spine is feasible. The temperature accuracy of the investigated T1- and PRF-based MRTh methods in vitro is high enough and promises to be reliable in vivo as well.

Spatial and temporal differences in traffic-related air pollution in three urban neighborhoods near an interstate highway.

PubMed

Patton, Allison P; Perkins, Jessica; Zamore, Wig; Levy, Jonathan I; Brugge, Doug; Durant, John L

2014-12-01

Relatively few studies have characterized differences in intra- and inter-neighborhood traffic-related air pollutant (TRAP) concentrations and distance-decay gradients in along an urban highway for the purposes of exposure assessment. The goal of this work was to determine the extent to which intra- and inter-neighborhood differences in TRAP concentrations can be explained by traffic and meteorology in three pairs of neighborhoods along Interstate 93 (I-93) in the metropolitan Boston area (USA). We measured distance-decay gradients of seven TRAPs (PNC, pPAH, NO, NO X , BC, CO, PM 2.5 ) in near-highway (<400 m) and background areas (>1 km) in Somerville, Dorchester/South Boston, Chinatown and Malden to determine whether (1) spatial patterns in concentrations and inter-pollutant correlations differ between neighborhoods, and (2) variation within and between neighborhoods can be explained by traffic and meteorology. The neighborhoods ranged in area from 0.5 to 2.3 km 2 . Mobile monitoring was performed over the course of one year in each pair of neighborhoods (one pair of neighborhoods per year in three successive years; 35-47 days of monitoring in each neighborhood). Pollutant levels generally increased with highway proximity, consistent with I-93 being a major source of TRAP; however, the slope and extent of the distance-decay gradients varied by neighborhood as well as by pollutant, season and time of day. Correlations among pollutants differed between neighborhoods (e.g., ρ = 0.35-0.80 between PNC and NO X and ρ = 0.11-0.60 between PNC and BC) and were generally lower in Dorchester/South Boston than in the other neighborhoods. We found that the generalizability of near-road gradients and near-highway/urban background contrasts was limited for near-highway neighborhoods in a metropolitan area with substantial local street traffic. Our findings illustrate the importance of measuring gradients of multiple pollutants under different ambient conditions in individual near-highway neighborhoods for health studies involving inter-neighborhood comparisons.

Spatial and temporal differences in traffic-related air pollution in three urban neighborhoods near an interstate highway

PubMed Central

Patton, Allison P.; Perkins, Jessica; Zamore, Wig; Levy, Jonathan I.; Brugge, Doug; Durant, John L.

2014-01-01

Relatively few studies have characterized differences in intra- and inter-neighborhood traffic-related air pollutant (TRAP) concentrations and distance-decay gradients in along an urban highway for the purposes of exposure assessment. The goal of this work was to determine the extent to which intra- and inter-neighborhood differences in TRAP concentrations can be explained by traffic and meteorology in three pairs of neighborhoods along Interstate 93 (I-93) in the metropolitan Boston area (USA). We measured distance-decay gradients of seven TRAPs (PNC, pPAH, NO, NOX, BC, CO, PM2.5) in near-highway (<400 m) and background areas (>1 km) in Somerville, Dorchester/South Boston, Chinatown and Malden to determine whether (1) spatial patterns in concentrations and inter-pollutant correlations differ between neighborhoods, and (2) variation within and between neighborhoods can be explained by traffic and meteorology. The neighborhoods ranged in area from 0.5 to 2.3 km2. Mobile monitoring was performed over the course of one year in each pair of neighborhoods (one pair of neighborhoods per year in three successive years; 35-47 days of monitoring in each neighborhood). Pollutant levels generally increased with highway proximity, consistent with I-93 being a major source of TRAP; however, the slope and extent of the distance-decay gradients varied by neighborhood as well as by pollutant, season and time of day. Correlations among pollutants differed between neighborhoods (e.g., ρ = 0.35-0.80 between PNC and NOX and ρ = 0.11-0.60 between PNC and BC) and were generally lower in Dorchester/South Boston than in the other neighborhoods. We found that the generalizability of near-road gradients and near-highway/urban background contrasts was limited for near-highway neighborhoods in a metropolitan area with substantial local street traffic. Our findings illustrate the importance of measuring gradients of multiple pollutants under different ambient conditions in individual near-highway neighborhoods for health studies involving inter-neighborhood comparisons. PMID:25364295

Creep and Environmental Durability of EBC/CMCs Under Imposed Thermal Gradient Conditions

NASA Technical Reports Server (NTRS)

Appleby, Matthew; Morscher, Gregory N.; Zhu, Dongming

2013-01-01

Interest in SiC fiber-reinforced SiC ceramic matrix composite (CMC) environmental barrier coating (EBC) systems for use in high temperature structural applications has prompted the need for characterization of material strength and creep performance under complex aerospace turbine engine environments. Stress-rupture tests have been performed on SiC/SiC composites systems, with varying fiber types and coating schemes to demonstrate material behavior under isothermal conditions. Further testing was conducted under exposure to thermal stress gradients to determine the effect on creep resistance and material durability. In order to understand the associated damage mechanisms, emphasis is placed on experimental techniques as well as implementation of non-destructive evaluation; including electrical resistivity monitoring. The influence of environmental and loading conditions on life-limiting material properties is shown.

Environmental Durability and Stress Rupture of EBC/CMCs

NASA Technical Reports Server (NTRS)

Appleby, Matthew; Morscher, Gregory N.; Zhu, Dongming

2012-01-01

This research focuses on the strength and creep performance of SiC fiber-reinforced SiC ceramic matrix composite (CMC) environmental barrier coating (EBC) systems under complex simulated engine environments. Tensile-strength and stress-rupture testing was conducted to illustrate the material properties under isothermal and thermal gradient conditions. To determine material durability, further testing was conducted under exposure to thermal cycling, thermal gradients and simulated combustion environments. Emphasis is placed on experimental techniques as well as implementation of non-destructive evaluation, including modal acoustic emission and electrical resistivity monitoring, to characterize strength degradation and damage mechanisms. Currently, little is known about the behavior of EBC-CMCs under these conditions; consequently, this work will prove invaluable in the development of structural components for use in high temperature applications.

Evaluating Interannual Variability of Accumulation Gradients on the Juneau Icefield

NASA Astrophysics Data System (ADS)

Koncewicz, E.; Bollen, K.; Burkhart, A.; Cabrera, V.; Rovzar, T.; Truax, O.; McNeil, C.; Nicholson, L. I.; O'Neel, S.

2016-12-01

The Juneau Icefield Research Program has collected mass balance data over the last 70 years on the Taku and Lemon Creek glaciers. We analyze data from 2004-2016 to investigate the interannual variability in the accumulation gradients of these two glaciers from ground penetrating radar (GPR), probing, and snow pits. Understanding interannual variability of accumulation gradients on the Juneau Icefield will help us to interpret its long-term mass balance record. The Lemon Creek Glacier is a small valley glacier on the southwest edge of the Icefield. GPR data was collected over the glacier surface in March 2015 and 2016. In July of 2014 and 2016, the accumulation area was probed for snow depth, and two snow pits were dug for snow depth and density. The accumulation gradients resulting from each method are compared between years to assess the interannnual variability of the accumulation gradient and the resulting glacier wide mass balance. The Taku Glacier is the largest outlet glacier on the Juneau Icefield. We use three snow pits dug each year along the longitudinal profile of the glacier between 1000m and 1115m, the region that typically reflects the ELA. In 2004, 2005, 2010, 2011, and 2016, snow probing was continued in the central region of the Taku and the resulting gradients are compared to each other and to the gradients derived from the snow pits. We assess the resulting impact on glacier wide mass balance furthering our understanding of the state of these two well-monitored glaciers on the Juneau Icefield.

Groundwater Monitoring and Engineered Geothermal Systems: The Newberry EGS Demonstration

NASA Astrophysics Data System (ADS)

Grasso, K.; Cladouhos, T. T.; Garrison, G.

2013-12-01

Engineered Geothermal Systems (EGS) represent the next generation of geothermal energy development. Stimulation of multiple zones within a single geothermal reservoir could significantly reduce the cost of geothermal energy production. Newberry Volcano in central Oregon represents an ideal location for EGS research and development. As such, the goals of the Newberry EGS Demonstration, operated by AltaRock Energy, Inc., include stimulation of a multiple-zone EGS reservoir, testing of single-well tracers and a demonstration of EGS reservoir viability through flow-back and circulation tests. A shallow, local aquifer supplied the approximately 41,630 m3 (11 million gals) of water used during stimulation of NWG 55-29, a deep geothermal well on the western flank of Newberry Volcano. Protection of the local aquifer is of primary importance to both the Newberry EGS Demonstration and the public. As part of the Demonstration, AltaRock Energy, Inc. has developed and implemented a groundwater monitoring plan to characterize the geochemistry of the local aquifer before, during and after stimulation. Background geochemical conditions were established prior to stimulation of NWG 55-29, which was completed in 2012. Nine sites were chosen for groundwater monitoring. These include the water supply well used during stimulation of NWG 55-29, three monitoring wells, three domestic water wells and two hot seeps located in the Newberry Caldera. Together, these nine monitoring sites represent up-, down- and cross-gradient locations. Groundwater samples are analyzed for 25 chemical constituents, stable isotopes, and geothermal tracers used during stimulation. In addition, water level data is collected at three monitoring sites in order to better characterize the effects of stimulation on the shallow aquifer. To date, no significant geochemical changes and no geothermal tracers have been detected in groundwater samples from these monitoring sites. The Newberry EGS Demonstration groundwater monitoring program is currently on-going.

Analysis of Measurement Error and Estimator Shape in Three-Point Hydraulic Gradient Estimators

NASA Astrophysics Data System (ADS)

McKenna, S. A.; Wahi, A. K.

2003-12-01

Three spatially separated measurements of head provide a means of estimating the magnitude and orientation of the hydraulic gradient. Previous work with three-point estimators has focused on the effect of the size (area) of the three-point estimator and measurement error on the final estimates of the gradient magnitude and orientation in laboratory and field studies (Mizell, 1980; Silliman and Frost, 1995; Silliman and Mantz, 2000; Ruskauff and Rumbaugh, 1996). However, a systematic analysis of the combined effects of measurement error, estimator shape and estimator orientation relative to the gradient orientation has not previously been conducted. Monte Carlo simulation with an underlying assumption of a homogeneous transmissivity field is used to examine the effects of uncorrelated measurement error on a series of eleven different three-point estimators having the same size but different shapes as a function of the orientation of the true gradient. Results show that the variance in the estimate of both the magnitude and the orientation increase linearly with the increase in measurement error in agreement with the results of stochastic theory for estimators that are small relative to the correlation length of transmissivity (Mizell, 1980). Three-point estimator shapes with base to height ratios between 0.5 and 5.0 provide accurate estimates of magnitude and orientation across all orientations of the true gradient. As an example, these results are applied to data collected from a monitoring network of 25 wells at the WIPP site during two different time periods. The simulation results are used to reduce the set of all possible combinations of three wells to those combinations with acceptable measurement errors relative to the amount of head drop across the estimator and base to height ratios between 0.5 and 5.0. These limitations reduce the set of all possible well combinations by 98 percent and show that size alone as defined by triangle area is not a valid discriminator of whether or not the estimator provides accurate estimates of the gradient magnitude and orientation. This research was funded by WIPP programs administered by the U.S Department of Energy. Sandia is a multiprogram laboratory operated by Sandia Corporation, a Lockheed Martin Company, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

Wilson Corners SWMU 001 2015 Annual Long Term Monitoring Report Kennedy Space Center, Florida

NASA Technical Reports Server (NTRS)

Lawson, Emily M.

2016-01-01

This document presents the findings of the 2015 Long Term Monitoring (LTM) that was completed at the Wilson Corners site, located at the National Aeronautics and Space Administration John F. Kennedy Space Center, Florida. The objectives of the 2015 LTM event were to evaluate the groundwater flow direction and gradient, to monitor the vertical and horizontal extent of the volatile organic compounds (VOCs; including the upgradient and sidegradient extents, which are monitored every five years), and to monitor select locations internal to the dissolved groundwater plume. The 2015 LTM event included several upgradient and sidegradient monitoring wells that are not sampled annually to verify the extent of VOCs in this portion of the site. The December 2015 LTM groundwater sampling event included, depth to groundwater measurements, 40 VOC samples collected using passive diffusion bags, and one VOC sample collected using low-flow techniques. Additionally, monitoring well MW0052DD was overdrilled and abandoned using rotasonic drilling techniques. The following conclusions can be made based on the 2015 LTM results: groundwater flow is generally to the west with northwest and southwest flow components from the water table to approximately 55 feet below land surface (ft BLS); peripheral monitoring wells generally delineate VOCs to groundwater cleanup target levels (GCTLs) except for monitoring wells MW0088, MW0090, MW0095, and NPSHMW0039, which had vinyl chloride (VC) concentrations near the GCTL and MW0062, which had trichloroethene (TCE), cis-1,2-dichloroethenen (cDCE), and VC concentrations above natural attenuation default concentrations (NADCs); VOCs in interior downgradient wells generally fluctuate within historic ranges except for monitoring wells in the north-northwest portion of the site, which have increasing VC concentrations indicating potential plume migration and expansion; Historically, the vertical extents of the VOCs were delineated by monitoring wells screened greater than 60 ft BLS (MW0083 through MW0086, and MW0078). The 2015 LTM results indicate that concentrations of daughter product cDCE is greater than the NADC in MW0078 and that cDCE and VC are greater than NADCs in MW0130. TCE was greater than the GCTL in monitoring well MW130 and not detected above method detection limits (9 micrograms per Liter) in monitoring well MW0078. No GCTL exceedances were identified in monitoring wells MW0083 or MW0086; the dissolved plume footprint appears generally stable, though not fully delineated by monitoring well data in the northeast portion of the site; and 2015 LTM results generally support the existing Conceptual Site Model. Geosyntec recommends modifying the LTM program, collecting a verification sample from monitoring well MW0062, and performing a direct push technology instigation in the northnortheastern portion of the site. A cluster of monitoring wells (MW0132 [2 to 12 ft BLS], MW0133 [15 to 25 ft BLS], and MW0134 [29 to 34 ft BLS]) is proposed to delineate impacts in the northnortheast portion of the site. Geosyntec recommends installation of a vertical extent well in the center of the site (Hot Spot 2 area) post-remediation implementation.

Pharmacokinetics-on-a-Chip Using Label-Free SERS Technique for Programmable Dual-Drug Analysis.

PubMed

Fei, Jiayuan; Wu, Lei; Zhang, Yizhi; Zong, Shenfei; Wang, Zhuyuan; Cui, Yiping

2017-06-23

Synergistic effects of dual or multiple drugs have attracted great attention in medical fields, especially in cancer therapies. We provide a programmable microfluidic platform for pharmacokinetic detection of multiple drugs in multiple cells. The well-designed microfluidic platform includes two 2 × 3 microarrays of cell chambers, two gradient generators, and several pneumatic valves. Through the combined use of valves and gradient generators, each chamber can be controlled to infuse different kinds of living cells and drugs with specific concentrations as needed. In our experiments, 6-mercaptopurine (6MP) and methimazole (MMI) were chosen as two drug models and their pharmacokinetic parameters in different living cells were monitored through intracellular SERS spectra, which reflected the molecular structure of these drugs. The dynamic change of SERS fingerprints from 6MP and MMI molecules were recorded during drug metabolism in living cells. The results indicated that both 6MP and MMI molecules were diffused into the cells within 4 min and excreted out after 36 h. Moreover, the intracellular distribution of these drugs was monitored through SERS mapping. Thus, our microfluidic platform simultaneously accomplishes the functions to monitor pharmacokinetic action, distribution, and fingerprint of multiple drugs in multiple cells. Owing to its real-time, rapid-speed, high-precision, and programmable capability of multiple-drug and multicell analysis, such a microfluidic platform has great potential in drug design and development.

Monitoring of internal pH gradients within multi-layer tablets by optical methods and EPR imaging.

PubMed

Eisenächer, Friederike; Schädlich, Andreas; Mäder, Karsten

2011-09-30

The high variability of gastrointestinal pH is a general challenge regarding constant release from oral drug delivery systems, especially for ionisable drugs. These drugs often show a pH-dependent solubility and therewith associated intra- and inter-individual variability of emerging drug plasma levels. Several strategies have been investigated with the intention to influence the microenvironmental pH (pH(M)) within solid formulations and therefore achieve pH-independent release profiles. Because of the heterogeneity of solid systems, a precise prediction of the occurring pH(M) is rather difficult. It is therefore important to monitor the pH(M) within the formulations to achieve requested release as well as to minimise pH-dependent degradation processes of the active compound. The purpose of the current study was the analysis of pH(M) gradients within 2- and 3-layer tablets during hydration using 3 different techniques for comparison intensions, in particular a pH indicator dye, fluorescence imaging and EPR imaging. The influence of the presence or absence of pH modifying substances and of an additional lipophilic inter layer on the pH(M) was investigated as well as the variation of matrix forming excipient and buffer pH. The influence of the pH(M) on drug release was analysed as well. In addition, benchtop MRI was accomplished to gain a deeper insight on the hydration and erosion behaviour of 2- and 3-layer tablets. Copyright © 2010 Elsevier B.V. All rights reserved.

Application of ERT, Saline Tracer and Numerical Studies to Delineate Preferential Paths in Fractured Granites.

PubMed

Sreeparvathy, Vijay; Kambhammettu, B V N P; Peddinti, Srinivasa Rao; Sarada, P S L

2018-03-22

Accurate quantification of in situ heterogeneity and flow processes through fractured geologic media remains elusive for hydrogeologists due to the complexity in fracture characterization and its multiscale behavior. In this research, we demonstrated the efficacy of tracer-electrical resistivity tomography (ERT) experiments combined with numerical simulations to characterize heterogeneity and delineate preferential flow paths in a fractured granite aquifer. A series of natural gradient saline tracer experiments were conducted from a depth window of 18 to 22 m in an injection well (IW) located inside the Indian Institute of Technology Hyderabad campus. Tracer migration was monitored in a time-lapse mode using two cross-sectional surface ERT profiles placed in the direction of flow gradient. ERT data quality was improved by considering stacking, reciprocal measurements, resolution indicators, and geophysical logs. Dynamic changes in subsurface electrical properties inferred via resistivity anomalies were used to highlight preferential flow paths of the study area. Temporal changes in electrical resistivity and tracer concentration were monitored along the vertical in an observation well located at 48 m to the east of the IW. ERT-derived tracer breakthrough curves were in agreement with geochemical sample measurements. Fracture geometry and hydraulic properties derived from ERT and pumping tests were further used to evaluate two mathematical conceptualizations that are relevant to fractured aquifers. Results of numerical analysis conclude that dual continuum model that combines matrix and fracture systems through a flow exchange term has outperformed equivalent continuum model in reproducing tracer concentrations at the monitoring wells (evident by a decrease in RMSE from 199 to 65 mg/L). A sensitivity analysis on model simulations conclude that spatial variability in hydraulic conductivity, local-scale dispersion, and flow exchange at fracture-matrix interface have a profound effect on model simulations. © 2018, National Ground Water Association.

Geophysical Modeling in Eurasia: 2D Crustal P and LG Propagation; Upper- Mantle Shear Wave Propagation and Anisotropy; and 3D, Joint, Simultaneous Inversions

DTIC Science & Technology

2008-09-01

improved resolution for shallow geologic structures . Jointly inverting these datasets with seismic body wave (S) travel times provides additional...constraints on the shallow structure and an enhanced 3D shear wave model for our study area in western China. 2008 Monitoring Research Review...for much of Eurasia, although the Arabian Shield and Arctic are less well recovered. The upper velocity gradient was tested for 10-degree cells

Ion-exchange chromatography purification of extracellular vesicles.

PubMed

Kosanović, Maja; Milutinović, Bojana; Goč, Sanja; Mitić, Ninoslav; Janković, Miroslava

2017-08-01

Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification of EVs. EVs in bulk were isolated from pooled normal human amniotic fluid (AF) by differential centrifugation followed by IEC or sucrose density gradient separation. The purity of the isolated EVs was evaluated by electrophoresis and lectin blotting/immuno blotting to monitor the distribution of total proteins, different EVs markers, and selected N-glycans. Our data showed efficient separation of negatively charged EVs from other differently charged molecules, while comparative profiling of EVs using SDG centrifugation confirmed anion-exchange chromatography is advantageous for EV purification. Finally, although this IEC-based method was validated using AF, the approach should be readily applicable to isolation of EVs from other sources as well.

Flow field and dissolved oxygen distributions in the outer channel of the Orbal oxidation ditch by monitor and CFD simulation.

PubMed

Guo, Xuesong; Zhou, Xin; Chen, Qiuwen; Liu, Junxin

2013-04-01

In the Orbal oxidation ditch, denitrification is primarily accomplished in the outer channel. However, the detailed characteristics of the flow field and dissolved oxygen (DO) distribution in the outer channel are not well understood. Therefore, in this study, the flow velocity and DO concentration in the outer channel of an Orbal oxidation ditch system in a wastewater treatment plant in Beijing (China) were monitored under actual operation conditions. The flow field and DO concentration distributions were analyzed by computed fluid dynamic modeling. In situ monitoring and modeling both showed that the flow velocity was heterogeneous in the outer channel. As a result, the DO was also heterogeneously distributed in the outer channel, with concentration gradients occurring along the flow direction as well as in the cross-section. This heterogeneous DO distribution created many anoxic and aerobic zones, which may have facilitated simultaneous nitrification-denitrification in the channel. These findings may provide supporting information for rational optimization of the performance of the Orbal oxidation ditch.

2012 Annual Industrial Wastewater Reuse Report for the Idaho National Laboratory Site’s Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mike Lewis

2013-02-01

This report describes conditions, as required by the state of Idaho Industrial Wastewater Reuse Permit (WRU-I-0160-01, formerly LA 000160 01), for the wastewater reuse site at the Idaho National Laboratory Site’s Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond from November 1, 2011 through October 31, 2012. The report contains the following information: • Facility and system description • Permit required effluent monitoring data and loading rates • Groundwater monitoring data • Status of special compliance conditions • Discussion of the facility’s environmental impacts During the 2012 reporting year, an estimated 11.84 million gallons of wastewater weremore » discharged to the Industrial Waste Ditch and Pond which is well below the permit limit of 17 million gallons per year. The concentrations of all permit-required analytes in the samples from the down gradient monitoring wells were below the Ground Water Quality Rule Primary and Secondary Constituent Standards.« less

2014 Annual Industrial Wastewater Reuse Report for the Idaho National Laboratory Site’s Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lewis, Mike

This report describes conditions, as required by the state of Idaho Industrial Wastewater Reuse Permit (WRU-I-0160-01, formerly LA 000160 01), for the wastewater reuse site at the Idaho National Laboratory Site’s Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond from November 1, 2013 through October 31, 2014. The report contains the following information; Facility and system description; Permit required effluent monitoring data and loading rates; Groundwater monitoring data; Status of special compliance conditions; Noncompliance issues; and Discussion of the facility’s environmental impacts During the 2014 reporting year, an estimated 10.11 million gallons of wastewater were discharged tomore » the Industrial Waste Ditch and Pond which is well below the permit limit of 17 million gallons per year. The concentrations of all permit-required analytes in the samples from the down gradient monitoring wells were below the applicable Idaho Department of Environmental Quality’s groundwater quality standard levels.« less

2013 Annual Industrial Wastewater Reuse Report for the Idaho National Laboratory Site’s Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mike Lewis

This report describes conditions, as required by the state of Idaho Industrial Wastewater Reuse Permit (WRU-I-0160-01, formerly LA 000160 01), for the wastewater reuse site at the Idaho National Laboratory Site’s Materials and Fuels Complex Industrial Waste Ditch and Industrial Waste Pond from November 1, 2012 through October 31, 2013. The report contains the following information: • Facility and system description • Permit required effluent monitoring data and loading rates • Groundwater monitoring data • Status of special compliance conditions • Discussion of the facility’s environmental impacts During the 2013 reporting year, an estimated 9.64 million gallons of wastewater weremore » discharged to the Industrial Waste Ditch and Pond which is well below the permit limit of 17 million gallons per year. The concentrations of all permit-required analytes in the samples from the down gradient monitoring wells were below the applicable Idaho Department of Environmental Quality’s groundwater quality standard levels.« less

43 CFR 3252.13 - How long may I collect information from my temperature gradient well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How long may I collect information from my temperature gradient well? 3252.13 Section 3252.13 Public Lands: Interior Regulations Relating to Public Lands... temperature gradient well? You may collect information from your temperature gradient well for as long as your...

43 CFR 3252.13 - How long may I collect information from my temperature gradient well?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How long may I collect information from my temperature gradient well? 3252.13 Section 3252.13 Public Lands: Interior Regulations Relating to Public Lands... temperature gradient well? You may collect information from your temperature gradient well for as long as your...

43 CFR 3252.13 - How long may I collect information from my temperature gradient well?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How long may I collect information from my temperature gradient well? 3252.13 Section 3252.13 Public Lands: Interior Regulations Relating to Public Lands... temperature gradient well? You may collect information from your temperature gradient well for as long as your...

43 CFR 3252.13 - How long may I collect information from my temperature gradient well?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How long may I collect information from my temperature gradient well? 3252.13 Section 3252.13 Public Lands: Interior Regulations Relating to Public Lands... temperature gradient well? You may collect information from your temperature gradient well for as long as your...

Compensating for magnetic field inhomogeneity in multigradient-echo-based MR thermometry.

PubMed

Simonis, Frank F J; Petersen, Esben T; Bartels, Lambertus W; Lagendijk, Jan J W; van den Berg, Cornelis A T

2015-03-01

MR thermometry (MRT) is a noninvasive method for measuring temperature that can potentially be used for radio frequency (RF) safety monitoring. This application requires measuring absolute temperature. In this study, a multigradient-echo (mGE) MRT sequence was used for that purpose. A drawback of this sequence, however, is that its accuracy is affected by background gradients. In this article, we present a method to minimize this effect and to improve absolute temperature measurements using MRI. By determining background gradients using a B0 map or by combining data acquired with two opposing readout directions, the error can be removed in a homogenous phantom, thus improving temperature maps. All scans were performed on a 3T system using ethylene glycol-filled phantoms. Background gradients were varied, and one phantom was uniformly heated to validate both compensation approaches. Independent temperature recordings were made with optical probes. Errors correlated closely to the background gradients in all experiments. Temperature distributions showed a much smaller standard deviation when the corrections were applied (0.21°C vs. 0.45°C) and correlated well with thermo-optical probes. The corrections offer the possibility to measure RF heating in phantoms more precisely. This allows mGE MRT to become a valuable tool in RF safety assessment. © 2014 Wiley Periodicals, Inc.

IMPROVED TEMPERATURE GRADIENT FOR MONITORING BEHAVIORAL THERMOREGULATION IN THE RAT

EPA Science Inventory

Past studies have found that the laboratory rat placed In a temperature gradient prefers temperatures that are markedly below its lower critical ambient temperature (LCT), whereas other rodents (e.g., mouse, hamster, and guinea pig) generally select thermal environments associate...

Temperature logging of groundwater in bedrock wells for geothermal gradient characterization in New Hampshire, 2012

USGS Publications Warehouse

Degnan, James; Barker, Gregory; Olson, Neil; Wilder, Leland

2012-01-01

Maximum groundwater temperatures at the bottom of the logs were between 11.7 and 17.3 degrees Celsius. Geothermal gradients were generally higher than typically reported for other water wells in the United States. Some of the high gradients were associated with high natural gamma emissions. Groundwater flow was discernible in 5 of the 10 wells studied but only obscured the portion of the geothermal gradient signal where groundwater actually flowed through the well. Temperature gradients varied by mapped bedrock type but can also vary by differences in mineralogy or rock type within the wells.

43 CFR 3252.14 - How must I complete a temperature gradient well?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How must I complete a temperature gradient... LEASING Conducting Exploration Operations § 3252.14 How must I complete a temperature gradient well? Complete temperature gradient wells to allow for proper abandonment, and to prevent interzonal migration of...

43 CFR 3252.14 - How must I complete a temperature gradient well?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How must I complete a temperature gradient... LEASING Conducting Exploration Operations § 3252.14 How must I complete a temperature gradient well? Complete temperature gradient wells to allow for proper abandonment, and to prevent interzonal migration of...

43 CFR 3252.14 - How must I complete a temperature gradient well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How must I complete a temperature gradient... LEASING Conducting Exploration Operations § 3252.14 How must I complete a temperature gradient well? Complete temperature gradient wells to allow for proper abandonment, and to prevent interzonal migration of...

43 CFR 3252.14 - How must I complete a temperature gradient well?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How must I complete a temperature gradient... LEASING Conducting Exploration Operations § 3252.14 How must I complete a temperature gradient well? Complete temperature gradient wells to allow for proper abandonment, and to prevent interzonal migration of...

Evaluation of geophysical logs, phase I, for Crossley Farms Superfund Site, Berks County, Pennsylvania

USGS Publications Warehouse

Conger, Randall W.

1998-01-01

Twenty-one wells were drilled at Crossley Farms Superfund Site between December 15, 1987, and May 1, 1988, to define and monitor the horizontal and vertical distribution of ground-water contamination emanating from a suspected contaminant source area (Blackhead Hill). Eight well clusters were drilled on or near the Crossley Site and three well clusters were drilled at locations hydrologically down gradient from the site. Depths of wells range from 21 to 299 feet below land surface. These wells were installed in saprolite in shallow, intermediate, and deep water-producing zones of the fractured bedrock aquifer. Borehole-geophysical and video logging were conducted between April 24, 1997, and May 8, 1997, to determine the water-producing zones, water-receiving zones, zones of vertical flow, borehole depth, and casing integrity in each well. This data and interpretation will be used to determine the location of the well intake for the existing open-hole wells, which will be retrofitted to isolate and monitor water-producing zones and prevent further cross-contamination within each open borehole, and identify wells that may need rehabilitation or replacement. Caliper and video logs were used to locate fractures, inflections on fluid-temperature and fluidresistivity logs indicated possible fluid-bearing fractures, and flowmeter measurements verified these locations. Single-point-resistance and natural-gamma logs provided information on stratigraphy. After interpretation of geophysical logs, video logs, and driller?s notes, all wells will be constructed so that water-level fluctuations can be monitored and discrete water samples collected from shallow, intermediate, and deep water-bearing zones in each well. Geophysical logs were run on seven bedrock and two deep bedrock wells. Gamma logs were run on 10 bedrock wells. Twenty-two wells were inspected visually with the borehole video camera for casing integrity.

Mapping Brazilian savanna vegetation gradients with Landsat time series

NASA Astrophysics Data System (ADS)

Schwieder, Marcel; Leitão, Pedro J.; da Cunha Bustamante, Mercedes Maria; Ferreira, Laerte Guimarães; Rabe, Andreas; Hostert, Patrick

2016-10-01

Global change has tremendous impacts on savanna systems around the world. Processes related to climate change or agricultural expansion threaten the ecosystem's state, function and the services it provides. A prominent example is the Brazilian Cerrado that has an extent of around 2 million km2 and features high biodiversity with many endemic species. It is characterized by landscape patterns from open grasslands to dense forests, defining a heterogeneous gradient in vegetation structure throughout the biome. While it is undisputed that the Cerrado provides a multitude of valuable ecosystem services, it is exposed to changes, e.g. through large scale land conversions or climatic changes. Monitoring of the Cerrado is thus urgently needed to assess the state of the system as well as to analyze and further understand ecosystem responses and adaptations to ongoing changes. Therefore we explored the potential of dense Landsat time series to derive phenological information for mapping vegetation gradients in the Cerrado. Frequent data gaps, e.g. due to cloud contamination, impose a serious challenge for such time series analyses. We synthetically filled data gaps based on Radial Basis Function convolution filters to derive continuous pixel-wise temporal profiles capable of representing Land Surface Phenology (LSP). Derived phenological parameters revealed differences in the seasonal cycle between the main Cerrado physiognomies and could thus be used to calibrate a Support Vector Classification model to map their spatial distribution. Our results show that it is possible to map the main spatial patterns of the observed physiognomies based on their phenological differences, whereat inaccuracies occurred especially between similar classes and data-scarce areas. The outcome emphasizes the need for remote sensing based time series analyses at fine scales. Mapping heterogeneous ecosystems such as savannas requires spatial detail, as well as the ability to derive important phenological parameters for monitoring habitats or ecosystem responses to climate change. The open Landsat and Sentinel-2 archives provide the satellite data needed for improved analyses of savanna ecosystems globally.

A validated stability-indicating UPLC method for desloratadine and its impurities in pharmaceutical dosage forms.

PubMed

Rao, Dantu Durga; Satyanarayana, N V; Malleswara Reddy, A; Sait, Shakil S; Chakole, Dinesh; Mukkanti, K

2010-02-05

A novel stability-indicating gradient reverse phase ultra-performance liquid chromatographic (RP-UPLC) method was developed for the determination of purity of desloratadine in presence of its impurities and forced degradation products. The method was developed using Waters Aquity BEH C18 column with mobile phase containing a gradient mixture of solvents A and B. The eluted compounds were monitored at 280nm. The run time was 8min within which desloratadine and its five impurities were well separated. Desloratadine was subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation. Desloratadine was found to degrade significantly in oxidative and thermal stress conditions and stable in acid, base, hydrolytic and photolytic degradation conditions. The degradation products were well resolved from main peak and its impurities, thus proved the stability-indicating power of the method. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness. This method was also suitable for the assay determination of desloratadine in pharmaceutical dosage forms.

Ultrafast NMR diffusion measurements exploiting chirp spin echoes.

PubMed

Ahola, Susanna; Mankinen, Otto; Telkki, Ville-Veikko

2017-04-01

Standard diffusion NMR measurements require the repetition of the experiment multiple times with varying gradient strength or diffusion delay. This makes the experiment time-consuming and restricts the use of hyperpolarized substances to boost sensitivity. We propose a novel single-scan diffusion experiment, which is based on spatial encoding of two-dimensional data, employing the spin-echoes created by two successive adiabatic frequency-swept chirp π pulses. The experiment is called ultrafast pulsed-field-gradient spin-echo (UF-PGSE). We present a rigorous derivation of the echo amplitude in the UF-PGSE experiment, justifying the theoretical basis of the method. The theory reveals also that the standard analysis of experimental data leads to a diffusion coefficient value overestimated by a few per cent. Although the overestimation is of the order of experimental error and thus insignificant in many practical applications, we propose that it can be compensated by a bipolar gradient version of the experiment, UF-BP-PGSE, or by corresponding stimulated-echo experiment, UF-BP-pulsed-field-gradient stimulated-echo. The latter also removes the effect of uniform background gradients. The experiments offer significant prospects for monitoring fast processes in real time as well as for increasing the sensitivity of experiments by several orders of magnitude by nuclear spin hyperpolarization. Furthermore, they can be applied as basic blocks in various ultrafast multidimensional Laplace NMR experiments. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Geophysical logging of bedrock wells for geothermal gradient characterization in New Hampshire, 2013

USGS Publications Warehouse

Degnan, James R.; Barker, Gregory; Olson, Neil; Wilder, Leland

2014-01-01

Maximum groundwater temperatures at the bottom of the logs ranged from 11.2 to 15.4 degrees Celsius. Geothermal gradients were generally higher than those typically reported for other water wells in the United States. Some of the high gradients were associated with high natural gamma emissions. Groundwater flow was discernible in 4 of the 10 wells studied but only obscured the part of the geothermal gradient signal where groundwater actually flowed into, out of, or through the well. Temperature gradients varied by mapped bedrock type but can also vary by localized differences in mineralogy or rock type within the wells.

Morphological Evolution of Directional Solidification Interfaces in Microgravity: An Analysis of Model Experiments Performed on the International Space Station

NASA Technical Reports Server (NTRS)

Strutzenberg, Louise L.; Grugel, R. N.; Trivedi, R. K.

2005-01-01

A series of experiments performed using the Pore Formation and Mobility Investigation (PFMI) apparatus within the glovebox facility (GBX) on board the International Space Station (ISS) has provided video images of the morphological evolution of a three-dimensional interface in a diffusion controlled regime. The experimental samples were prepared on ground by filling glass tubes, 1 cm ID and approximately 30 cm in length, with "alloys" of succinonitrile (SCN) and water in an atmosphere of nitrogen at 450 millibar pressure. The compositions of the samples processed and analyzed are 0.25,0.5 and 1.0 wt% water. Experimental processing parameters of temperature gradient and translation speed, as well as camera settings, were remotely monitored and manipulated from the ground Telescience Center (TSC) at the Marshall !3pace Flight Center. During the experiments, the sample was first subjected to a unidirectional melt back, generally at 10 microns per second, with a constant temperature gradient ahead of the melting interface. Following the melt back, the interface was allowed to stabilize before translation is initiated. The temperatures in the sample were monitored by six in situ thermocouples and the position is monitored by an optical linear encoder. For the experiments performed and analyzed, the gradients ranged from 2.5 - 3.3 K/mm and the initial pulling velocities ranged from 0.7 micron per second to 1 micron per second with subsequent transition velocities of up to 100 microns per second. The data provided by the PFMI for analysis includes near-real-time (NRT) video captured on the ground during the experiment runs, ISS Video Tape Recorder (VTR) data dumped from the VTR at the end of the experiment run and recorded on the ground, telemetry data including temperature and position measurements, and limited flight HI-8 tapes in 2 camera views of experiment runs for which tapes have been returned to the investigators from ISS. Because of limited down mass from the ISS, the majority of the initial analysis has been performed using the NRT and VTR video data but will be supplemented with the HI-8 video as it becomes available. hardware and procedures required to prepare samples for correlation to flight samples is described. Using this ground-based system, a series of experiments have been performed for direct comparison with the flight data. The results of these comparisons as well as implications to future microgravity experiments are presented and discussed. A ground-based thin-sample directional solidification system as well as all associated

Long-Gradient Separations Coupled with Selected Reaction Monitoring for Highly Sensitive, Large Scale Targeted Protein Quantification in a Single Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shi, Tujin; Fillmore, Thomas L.; Gao, Yuqian

2013-10-01

Long-gradient separations coupled to tandem MS were recently demonstrated to provide a deep proteome coverage for global proteomics; however, such long-gradient separations have not been explored for targeted proteomics. Herein, we investigate the potential performance of the long-gradient separations coupled with selected reaction monitoring (LG-SRM) for targeted protein quantification. Direct comparison of LG-SRM (5 h gradient) and conventional LC-SRM (45 min gradient) showed that the long-gradient separations significantly reduced background interference levels and provided an 8- to 100-fold improvement in LOQ for target proteins in human female serum. Based on at least one surrogate peptide per protein, an LOQ ofmore » 10 ng/mL was achieved for the two spiked proteins in non-depleted human serum. The LG-SRM detection of seven out of eight endogenous plasma proteins expressed at ng/mL or sub-ng/mL levels in clinical patient sera was also demonstrated. A correlation coefficient of >0.99 was observed for the results of LG-SRM and ELISA measurements for prostate-specific antigen (PSA) in selected patient sera. Further enhancement of LG-SRM sensitivity was achieved by applying front-end IgY14 immunoaffinity depletion. Besides improved sensitivity, LG-SRM offers at least 3 times higher multiplexing capacity than conventional LC-SRM due to ~3-fold increase in average peak widths for a 300-min gradient compared to a 45-min gradient. Therefore, LG-SRM holds great potential for bridging the gap between global and targeted proteomics due to its advantages in both sensitivity and multiplexing capacity.« less

Groundwater level and specific conductance monitoring at Marine Corps Base, Camp Lejeune, Onslow County, North Carolina, 2007-2008

USGS Publications Warehouse

McSwain, Kristen Bukowski

2010-01-01

The U.S. Geological Survey, in cooperation with the Marine Corps Base, Camp Lejeune, monitored water-resources conditions in the surficial, Castle Hayne, Peedee, and Black Creek aquifers in Onslow County, North Carolina, from November 2007 through September 2008. To comply with North Carolina Central Coastal Plain Capacity Use Area regulations, large-volume water suppliers in Onslow County must reduce their dependency on the Black Creek aquifer as a water-supply source and have, instead, proposed using the Castle Hayne aquifer as an alternative water-supply source. The Marine Corps Base, Camp Lejeune, uses water obtained from the unregulated surficial and Castle Hayne aquifers for drinking-water supply. Water-level data were collected and field measurements of physical properties were made at 19 wells at 8 locations spanning the Marine Corps Base, Camp Lejeune. These wells were instrumented with near real-time monitoring equipment to collect hourly measurements of water level. Additionally, specific conductance and water temperature were measured hourly in 16 of the 19 wells. Graphs are presented relating altitude of groundwater level to water temperature and specific conductance measurements collected during the study, and the relative vertical gradients between aquifers are discussed. The period-of-record normal (25th to 75th percentile) monthly mean groundwater levels at two well clusters were compared to median monthly mean groundwater levels at these same well clusters for 2008 to determine groundwater-resources conditions. In 2008, water levels were below normal in the 3 wells at one of the well clusters and were normal in 4 wells at the other cluster.

Patterns in Temporal Variability of Temperature, Oxygen and pH along an Environmental Gradient in a Coral Reef

PubMed Central

Guadayol, Òscar; Silbiger, Nyssa J.; Donahue, Megan J.; Thomas, Florence I. M.

2014-01-01

Spatial and temporal environmental variability are important drivers of ecological processes at all scales. As new tools allow the in situ exploration of individual responses to fluctuations, ecologically meaningful ways of characterizing environmental variability at organism scales are needed. We investigated the fine-scale spatial heterogeneity of high-frequency temporal variability in temperature, dissolved oxygen concentration, and pH experienced by benthic organisms in a shallow coastal coral reef. We used a spatio-temporal sampling design, consisting of 21 short-term time-series located along a reef flat-to-reef slope transect, coupled to a long-term station monitoring water column changes. Spectral analyses revealed sharp gradients in variance decomposed by frequency, as well as differences between physically-driven and biologically-reactive parameters. These results highlight the importance of environmental variance at organismal scales and present a new sampling scheme for exploring this variability in situ. PMID:24416364

The dynamics of water in hydrated white bread investigated using quasielastic neutron scattering

NASA Astrophysics Data System (ADS)

Sjöström, J.; Kargl, F.; Fernandez-Alonso, F.; Swenson, J.

2007-10-01

The dynamics of water in fresh and in rehydrated white bread is studied using quasielastic neutron scattering (QENS). A diffusion constant for water in fresh bread, without temperature gradients and with the use of a non-destructive technique, is presented here for the first time. The self-diffusion constant for fresh bread is estimated to be Ds = 3.8 × 10-10 m2 s-1 and the result agrees well with previous findings for similar systems. It is also suggested that water exhibits a faster dynamics than previously reported in the literature using equilibration of a hydration-level gradient monitored by vibrational spectroscopy. The temperature dependence of the dynamics of low hydration bread is also investigated for T = 280-350 K. The average relaxation time at constant momentum transfer (Q) shows an Arrhenius behavior in the temperature range investigated.

Constrained optimization for position calibration of an NMR field camera.

PubMed

Chang, Paul; Nassirpour, Sahar; Eschelbach, Martin; Scheffler, Klaus; Henning, Anke

2018-07-01

Knowledge of the positions of field probes in an NMR field camera is necessary for monitoring the B 0 field. The typical method of estimating these positions is by switching the gradients with known strengths and calculating the positions using the phases of the FIDs. We investigated improving the accuracy of estimating the probe positions and analyzed the effect of inaccurate estimations on field monitoring. The field probe positions were estimated by 1) assuming ideal gradient fields, 2) using measured gradient fields (including nonlinearities), and 3) using measured gradient fields with relative position constraints. The fields measured with the NMR field camera were compared to fields acquired using a dual-echo gradient recalled echo B 0 mapping sequence. Comparisons were done for shim fields from second- to fourth-order shim terms. The position estimation was the most accurate when relative position constraints were used in conjunction with measured (nonlinear) gradient fields. The effect of more accurate position estimates was seen when compared to fields measured using a B 0 mapping sequence (up to 10%-15% more accurate for some shim fields). The models acquired from the field camera are sensitive to noise due to the low number of spatial sample points. Position estimation of field probes in an NMR camera can be improved using relative position constraints and nonlinear gradient fields. Magn Reson Med 80:380-390, 2018. © 2017 International Society for Magnetic Resonance in Medicine. © 2017 International Society for Magnetic Resonance in Medicine.

Using geo-targeted social media data to detect outdoor air pollution

NASA Astrophysics Data System (ADS)

Jiang, W.; Wang, Y.; Tsou, M. H.; Fu, X.

2016-06-01

Outdoor air pollution has become a more and more serious issue over recent years (He, 2014). Urban air quality is measured at air monitoring stations. Building air monitoring stations requires land, incurs costs and entails skilled technicians to maintain a station. Many countries do not have any monitoring stations and even lack any means to monitor air quality. Recent years, the social media could be used to monitor air quality dynamically (Wang, 2015; Mei, 2014). However, no studies have investigated the inter-correlations between real-space and cyberspace by examining variation in micro-blogging behaviors relative to changes in daily air quality. Thus, existing methods of monitoring AQI using micro-blogging data shows a high degree of error between real AQI and air quality as inferred from social media messages. In this paper, we introduce a new geo-targeted social media analytic method to (1) investigate the dynamic relationship between air pollution-related posts on Sina Weibo and daily AQI values; (2) apply Gradient Tree Boosting, a machine learning method, to monitor the dynamics of AQI using filtered social media messages. Our results expose the spatiotemporal relationships between social media messages and real-world environmental changes as well suggesting new ways to monitor air pollution using social media.

Monitoring shifts in plant diversity in response to climate change: A method for landscapes

USGS Publications Warehouse

Stohlgren, T.J.; Owen, A.J.; Lee, M.

2000-01-01

Improved sampling designs are needed to detect, monitor, and predict plant migrations and plant diversity changes caused by climate change and other human activities. We propose a methodology based on multi-scale vegetation plots established across forest ecotones which provide baseline data on patterns of plant diversity, invasions of exotic plant species, and plant migrations at landscape scales in Rocky Mountain National Park, Colorado, USA. We established forty two 1000-m2 plots in relatively homogeneous forest types and the ecotones between them on 14 vegetation transects. We found that 64% of the variance in understory species distributions at landscape scales were described generally by gradients of elevation and under-canopy solar radiation. Superimposed on broad-scale climatic gradients are small-scale gradients characterized by patches of light, pockets of fertile soil, and zones of high soil moisture. Eighteen of the 42 plots contained at least one exotic species; monitoring exotic plant invasions provides a means to assess changes in native plant diversity and plant migrations. Plant species showed weak affinities to overstory vegetation types, with 43% of the plant species found in three or more vegetation types. Replicate transects along several environmental gradients may provide the means to monitor plant diversity and species migrations at landscape scales because: (1) ecotones may play crucial roles in expanding the geophysiological ranges of many plant species; (2) low affinities of understory species to overstory forest types may predispose vegetation types to be resilient to rapid environmental change; and (3) ecotones may help buffer plant species from extirpation and extinction.

Monitoring and Risk Identification Caused by High Water, Floods and Erosion Processes in Urban Part of Sava Riverbed

NASA Astrophysics Data System (ADS)

Oskoruš, D.; Miković, N.; Ljevar, I.

2012-04-01

Riverbed erosion and bottom deepening are part of natural fluvial processes in the upper stream of Sava River. The increasing gradient of those changes is interconnected with the level of human influence in the river basin and riverbed as well. In time period of last forty years the consequences of riverbed erosion are become serious as well as dangerous and they threaten the stability of hydro technical structures. The increasing value of flow velocity in riverbed in urban part of river section during high water level, mud and debris flow during the floods as well, is especially dangerous for old bridges. This paper contains result of velocity measurements during high waters taken by Hydrological Service of Republic Croatia, load transport monitoring during such events and cross sections in some vulnerable location. In this paper is given one example of Jakuševac railway bridge in Zagreb, heavily destroyed during high water event on the 30 March 2009., recently reconstructed by "Croatian Railways" company. Keywords: Riverbed erosion, flow velocity, mud and debris flow, risk identification, stability of bridges

Experiment, monitoring, and gradient methods used to infer climate change effects on plant communities yield consistent patterns.

PubMed

Elmendorf, Sarah C; Henry, Gregory H R; Hollister, Robert D; Fosaa, Anna Maria; Gould, William A; Hermanutz, Luise; Hofgaard, Annika; Jónsdóttir, Ingibjörg S; Jónsdóttir, Ingibjörg I; Jorgenson, Janet C; Lévesque, Esther; Magnusson, Borgþór; Molau, Ulf; Myers-Smith, Isla H; Oberbauer, Steven F; Rixen, Christian; Tweedie, Craig E; Walker, Marilyn D; Walker, Marilyn

2015-01-13

Inference about future climate change impacts typically relies on one of three approaches: manipulative experiments, historical comparisons (broadly defined to include monitoring the response to ambient climate fluctuations using repeat sampling of plots, dendroecology, and paleoecology techniques), and space-for-time substitutions derived from sampling along environmental gradients. Potential limitations of all three approaches are recognized. Here we address the congruence among these three main approaches by comparing the degree to which tundra plant community composition changes (i) in response to in situ experimental warming, (ii) with interannual variability in summer temperature within sites, and (iii) over spatial gradients in summer temperature. We analyzed changes in plant community composition from repeat sampling (85 plant communities in 28 regions) and experimental warming studies (28 experiments in 14 regions) throughout arctic and alpine North America and Europe. Increases in the relative abundance of species with a warmer thermal niche were observed in response to warmer summer temperatures using all three methods; however, effect sizes were greater over broad-scale spatial gradients relative to either temporal variability in summer temperature within a site or summer temperature increases induced by experimental warming. The effect sizes for change over time within a site and with experimental warming were nearly identical. These results support the view that inferences based on space-for-time substitution overestimate the magnitude of responses to contemporary climate warming, because spatial gradients reflect long-term processes. In contrast, in situ experimental warming and monitoring approaches yield consistent estimates of the magnitude of response of plant communities to climate warming.

Short Diffusion Time Diffusion-Weighted Imaging With Oscillating Gradient Preparation as an Early Magnetic Resonance Imaging Biomarker for Radiation Therapy Response Monitoring in Glioblastoma: A Preclinical Feasibility Study.

PubMed

Bongers, Andre; Hau, Eric; Shen, Han

2018-01-04

To investigate a novel alternative diffusion-weighted imaging (DWI) approach using oscillating gradients preparation (OGSE) to obtain much shorter effective diffusion times (Δ eff ) for tumor response monitoring by apparent diffusion coefficient (ADC) mapping in a glioblastoma mouse model. Twenty-four BALB/c nude mice inoculated with U87 glioblastoma cells were randomized into a control group and an irradiation group, which underwent a 15-day fractioned radiation therapy (RT) course with 2 Gy/d. Therapy response was assessed by mapping of ADCs at 6 time points using an in-house implementation of a cos-OGSE DWI sequence with Δ eff  = 1.25 ms and compared with a standard pulsed gradient DWI protocol (PGSE) with typical clinical diffusion time Δ eff  = 18 ms. Longitudinal ADC changes in tumor and contralateral white matter (WM) were statistically assessed using repeated-measures analysis of variance and post hoc (Sidak) testing. On short Δ eff OGSE maps tumor ADC was generally 30%-50% higher than in surrounding WM. Areas correlated well with histology. Tumor identification was generally more difficult on PGSE maps owing to nonsignificant WM/tumor contrast. During RT, OGSE maps also showed significant tumor ADC increase (approximately 15%) in response to radiation, consistently seen after 14-Gy RT dose. The clinical reference (PGSE) showed lower sensitivity to radiation changes, and no significant response across the radiation group and time course could be detected. Our short Δ eff DWI method using OGSE better reflected histologically defined tumor areas and enabled more consistent and earlier detection of microstructural radiation changes than conventional methods. Oscillating gradients preparation offers significant potential as a robust microstructural RT response biomarker, potentially helping to shift important therapy decisions to earlier stages in the RT time course. Copyright © 2018 Elsevier Inc. All rights reserved.

Control - Demands mushroom as station grows

NASA Technical Reports Server (NTRS)

Szirmay, S. Z.; Blair, J.

1983-01-01

The NASA space station, which is presently in the planning stage, is to be composed of both rigid and nonrigid modules, rotating elements, and flexible appendages subjected to environmental disturbances from the earth's atmospheric gravity gradient, and magnetic field, as well as solar radiation and self-generated disturbances. Control functions, which will originally include attitude control, docking and berthing control, and system monitoring and management, will with evolving mission objectives come to encompass such control functions as articulation control, autonomous navigation, space traffic control, and large space structure control. Attention is given to the advancements in modular, distributed, and adaptive control methods, as well as system identification and hardware fault tolerance techniques, which will be required.

Monitoring and source tracking of tetracycline resistance genes in lagoons and groundwater adjacent to swine production facilities over a 3-year period

USGS Publications Warehouse

Koike, S.; Krapac, I.G.; Oliver, H.D.; Yannarell, A.C.; Chee-Sanford, J. C.; Aminov, R.I.; Mackie, R.I.

2007-01-01

To monitor the dissemination of resistance genes into the environment, we determined the occurrence of tetracycline resistance (Tcr) genes in groundwater underlying two swine confinement operations. Monitoring well networks (16 wells at site A and 6 wells at site C) were established around the lagoons at each facility. Groundwater (n = 124) and lagoon (n = 12) samples were collected from the two sites at six sampling times from 2000 through 2003. Total DNA was extracted, and PCR was used to detect seven Tcr genes [tet(M), tet(O), tet(Q), tet(W), tet(C), tet(H), and tet(Z)]. The concentration of Tcr genes was quantified by real-time quantitative PCR. To confirm the Tcr gene source in groundwater, comparative analysis of tet(W) gene sequences was performed on groundwater and lagoon samples. All seven Tcr genes were continually detected in groundwater during the 3-year monitoring period at both sites. At site A, elevated detection frequency and concentration of Tcr genes were observed in the wells located down-gradient of the lagoon. Comparative analysis of tet(W) sequences revealed that the impacted groundwater contained gene sequences almost identical (99.8% identity) to those in the lagoon, but these genes were not found in background libraries. Novel sequence clusters and unique indigenous resistance gene pools were also found in the groundwater. Thus, antibiotic resistance genes in groundwater are affected by swine manure, but they are also part of the indigenous gene pool. Copyright ?? 2007, American Society for Microbiology. All Rights Reserved.

Monitoring and Source Tracking of Tetracycline Resistance Genes in Lagoons and Groundwater Adjacent to Swine Production Facilities over a 3-Year Period▿

PubMed Central

Koike, S.; Krapac, I. G.; Oliver, H. D.; Yannarell, A. C.; Chee-Sanford, J. C.; Aminov, R. I.; Mackie, R. I.

2007-01-01

To monitor the dissemination of resistance genes into the environment, we determined the occurrence of tetracycline resistance (Tcr) genes in groundwater underlying two swine confinement operations. Monitoring well networks (16 wells at site A and 6 wells at site C) were established around the lagoons at each facility. Groundwater (n = 124) and lagoon (n = 12) samples were collected from the two sites at six sampling times from 2000 through 2003. Total DNA was extracted, and PCR was used to detect seven Tcr genes [tet(M), tet(O), tet(Q), tet(W), tet(C), tet(H), and tet(Z)]. The concentration of Tcr genes was quantified by real-time quantitative PCR. To confirm the Tcr gene source in groundwater, comparative analysis of tet(W) gene sequences was performed on groundwater and lagoon samples. All seven Tcr genes were continually detected in groundwater during the 3-year monitoring period at both sites. At site A, elevated detection frequency and concentration of Tcr genes were observed in the wells located down-gradient of the lagoon. Comparative analysis of tet(W) sequences revealed that the impacted groundwater contained gene sequences almost identical (99.8% identity) to those in the lagoon, but these genes were not found in background libraries. Novel sequence clusters and unique indigenous resistance gene pools were also found in the groundwater. Thus, antibiotic resistance genes in groundwater are affected by swine manure, but they are also part of the indigenous gene pool. PMID:17545324

Hydrogeological characterization of shallow-depth zone for CO2 injection and leak test at a CO2 environmental monitoring site in Korea

NASA Astrophysics Data System (ADS)

Lee, S. S.; Kim, T. W.; Kim, H. H.; Ha, S. W.; Jeon, W. T.; Lee, K. K.

2015-12-01

The main goal of the this study is to evaluate the importance of heterogeneities in controlling the field-scale transport of CO2 are originated from the CO2 injected at saturated zone below the water table for monitoring and prediction of CO2 leakage from a reservoir. Hydrogeological and geophysical data are collected to characterize the site, prior to conducting CO2 injection experiment at the CO2 environmental monitoring site at Eumseong, Korea. The geophysical data were acquired from borehole electromagnetic flowmeter tests, while the hydraulic data were obtained from pumping tests, slug tests, and falling head permeability tests. Total of 13 wells to perform hydraulic and geophysical test are established along groundwater flow direction in regular sequence, revealed by the results of borehole electromagnetic flowmeter test. The results of geophysical tests indicated that hydraulic gradient is not identical with the topographic gradient. Groundwater flows toward the uphill direction in the study area. Then, the hydraulic tests were conducted to identify the hydraulic properties of the study site. According to the results of pumping and slug tests at the study site, the hydraulic conductivity values show ranges between 4.75 x 10-5 cm/day and 9.74 x 10-5 cm/day. In addition, a portable multi-level sampling and monitoring packer device which remains inflated condition for a long period developed and used to isolate designated depths to identify vertical distribution of hydrogeological characteristics. Hydrogeological information obtained from this study will be used to decide the injection test interval of CO2-infused water and gaseous CO2. Acknowledgement: Financial support was provided by "R&D Project on Environmental Mangement of Geologic CO2 Storage" from the KEITI (Project Number: 2014001810003).

Vertical profile of tritium concentration in air during a chronic atmospheric HT release.

PubMed

Noguchi, Hiroshi; Yokoyama, Sumi

2003-03-01

The vertical profiles of tritium gas and tritiated water concentrations in air, which would have an influence on the assessment of tritium doses as well as on the environmental monitoring of tritium, were measured in a chronic tritium gas release experiment performed in Canada in 1994. While both of the profiles were rather uniform during the day because of atmospheric mixing, large gradients of the profiles were observed at night. The gradient coefficients of the profiles were derived from the measurements. Correlations were analyzed between the gradient coefficients and meteorological conditions: solar radiation, wind speed, and turbulent diffusivity. It was found that the solar radiation was highly correlated with the gradient coefficients of tritium gas and tritiated water profiles and that the wind speed and turbulent diffusivity showed weaker correlations with those of tritiated water profiles. A one-dimensional tritium transport model was developed to analyze the vertical diffusion of tritiated water re-emitted from the ground into the atmosphere. The model consists of processes of tritium gas deposition to soil including oxidation into tritiated water, reemission of tritiated water, dilution of tritiated water in soil by rain, and vertical diffusion of tritiated water in the atmosphere. The model accurately represents the accumulation of tritiated water in soil water and the time variations and vertical profiles of tritiated water concentrations in air.

2013 Annual Industrial Wastewater Reuse Report for the Idaho National Laboratory Site’s Advanced Test Reactor Complex Cold Waste Pond

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mike Lewis

2014-02-01

This report describes conditions, as required by the state of Idaho Industrial Wastewater Reuse Permit (#LA 000161 01, Modification B), for the wastewater land application site at the Idaho National Laboratory Site’s Advanced Test Reactor Complex Cold Waste Pond from November 1, 2012–October 31, 2013. The report contains the following information: • Facility and system description • Permit required effluent monitoring data and loading rates • Groundwater monitoring data • Status of compliance activities • Noncompliance issues • Discussion of the facility’s environmental impacts. During the 2013 permit year, approximately 238 million gallons of wastewater was discharged to the Coldmore » Waste Pond. This is well below the maximum annual permit limit of 375 million gallons. As shown by the groundwater sampling data, sulfate and total dissolved solids concentrations are highest near the Cold Waste Pond and decrease rapidly as the distance from the Cold Waste Pond increases. Although concentrations of sulfate and total dissolved solids are elevated near the Cold Waste Pond, both parameters are below the Ground Water Quality Rule Secondary Constituent Standards in the down gradient monitoring wells.« less

2012 Annual Industrial Wastewater Reuse Report for the Idaho National Laboratory Site's Advanced Test Reactor Complex Cold Waste Pond

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mike Lewis

2013-02-01

This report describes conditions, as required by the state of Idaho Industrial Wastewater Reuse Permit (#LA 000161 01, Modification B), for the wastewater land application site at the Idaho National Laboratory Site’s Advanced Test Reactor Complex Cold Waste Pond from November 1, 2011 through October 31, 2012. The report contains the following information: Facility and system description Permit required effluent monitoring data and loading rates Groundwater monitoring data Status of compliance activities Noncompliance issues Discussion of the facility’s environmental impacts During the 2012 permit year, approximately 183 million gallons of wastewater were discharged to the Cold Waste Pond. This ismore » well below the maximum annual permit limit of 375 million gallons. As shown by the groundwater sampling data, sulfate and total dissolved solids concentrations are highest near the Cold Waste Pond and decrease rapidly as the distance from the Cold Waste Pond increases. Although concentrations of sulfate and total dissolved solids are elevated near the Cold Waste Pond, both parameters were below the Ground Water Quality Rule Secondary Constituent Standards in the down gradient monitoring wells.« less

Damage Characterization of EBC-SiCSiC Ceramic Matrix Composites Under Imposed Thermal Gradient Testing

NASA Technical Reports Server (NTRS)

Appleby, Matthew P.; Morscher, Gregory N.; Zhu, Dongming

2014-01-01

Due to their high temperature capabilities, Ceramic Matrix Composite (CMC) components are being developed for use in hot-section aerospace engine applications. Harsh engine environments have led to the development of Environmental Barrier Coatings (EBCs) for silicon-based CMCs to further increase thermal and environmental capabilities. This study aims at understanding the damage mechanisms associated with these materials under simulated operating conditions. A high heat-flux laser testing rig capable of imposing large through-thickness thermal gradients by means of controlled laser beam heating and back-side air cooling is used. Tests are performed on uncoated composites, as well as CMC substrates that have been coated with state-of-the-art ceramic EBC systems. Results show that the use of the EBCs may help increase temperature capability and creep resistance by reducing the effects of stressed oxidation and environmental degradation. Also, the ability of electrical resistance (ER) and acoustic emission (AE) measurements to monitor material condition and damage state during high temperature testing is shown; suggesting their usefulness as a valuable health monitoring technique. Micromechanics models are used to describe the localized stress state of the composite system, which is utilized along with ER modeling concepts to develop an electromechanical model capable of characterizing material behavior.

Geologic, water-chemistry, and hydrologic data from multiple-well monitoring sites and selected water-supply wells in the Santa Clara Valley, California, 1999-2003

USGS Publications Warehouse

Newhouse, M.W.; Hanson, R.T.; Wentworth, C.M.; Everett, Rhett; Williams, C.F.; Tinsley, J.C.; Noce, T.E.; Carkin, B.A.

2004-01-01

To better identify the three-dimensional geohydrologic framework of the Santa Clara Valley, lithologic, geologic, geophysical, geomechanical, hydraulic, and water-chemistry data were collected from eight ground-water multiple-well monitoring sites constructed in Santa Clara County, California, as part of a series of cooperative studies between the U.S. Geological Survey and the Santa Clara Valley Water District. The data are being used to update and improve the three-dimensional geohydrologic framework of the basin and to address issues related to water supply, water chemistry, sequence stratigraphy, geology, and geological hazards. This report represents a compilation of data collected from 1999 to 2003, including location and design of the monitoring sites, cone penetrometer borings, geologic logs, lithologic logs, geophysical logs, core analysis, water-chemistry analysis, ground-water-level measurements, and hydraulic and geomechanical properties from wells and core samples. Exploratory cone penetrometer borings taken in the upper 17 to 130 feet at six of the monitoring sites identified the base of Holocene as no deeper than 75 feet in the central confined area and no deeper than 35 feet in the southern unconfined areas of the valley. Generalized lithologic characterization from the monitoring sites indicates about four to six different aquifer units separated by relatively fine-grained units occur within the alluvial deposits shallower than 860 feet deep. Analysis of geophysical logs indicates that coarse-grained units varied in thickness between 10 and 25 feet in the southeastern unconfined area of the valley and between 50 and 200 feet in the south-central and southwestern areas of the valley. Deviations from temperature-gradient logs indicate that the majority of horizontal ground-water flow occurs above a depth of 775 feet in the south central and above 510 feet in the southeastern areas of the valley. Bulk physical properties from more than 1,150 feet of core samples indicate an average primary-wave velocity of about 5,515 feet per second, a bimodal distribution of density between 2.19 and 2.32 grams per cubic centimeter with an average of 2.16 grams per cubic centimeter, and a magnetic susceptibility that generally ranged between 9 and 40 with an average of 22. Water-chemistry data indicate that the ground water in the alluvial aquifers generally is low in total dissolved solids and chloride and of good quality. Isotopic data indicate that water from artificial recharge is present throughout the shallower parts of the aquifer system but may not be present toward the center of the valley. The percentage of water from artificial recharge present in ground water ranges from 0 to 61 percent for water-supply wells. The age of most shallow ground water is less than 2,000 years before present, and the age of deeper ground water is as much as 39,900 years before present, as determined from carbon age dates. Initial water-level data from the multiple-well monitoring sites indicate seasonal water-level fluctuations as great as 60 feet and water-level differences between aquifers as great as 10 feet. The water-level hydrographs indicate different water-level changes and relations between aquifers in different parts of the basin. However, most of these hydrographs indicate the potential for downward water-level gradients, with lower hydraulic heads in the deeper monitoring wells. Hydraulic properties of selected new monitoring wells indicate that horizontal hydraulic conductivities range from 0.1 to 583 feet per day. Hydraulic testing of selected core samples yielded vertical hydraulic conductivity values ranging from 8 x 10-4 to 0.3 feet per day, and effective porosity values ranging from 0.21 to 0.4. Geomechanical properties estimated from one-dimensional consolidation tests of selected core samples resulted in geometric mean inelastic and elastic specific storage values of 1.5 x 10-

Microfluidic transwell inserts for generation of tissue culture-friendly gradients in well plates

PubMed Central

Sip, Christopher G.; Bhattacharjee, Nirveek; Folch, Albert

2015-01-01

Gradients of biochemical molecules play a key role in many physiological processes such as axon growth, tissue morphogenesis, and trans-epithelium nutrient transport, as well as in pathophysiological phenomena such as wound healing, immune response, bacterial invasion, and cancer metastasis. In this paper, we report a microfluidic transwell insert for generating quantifiable concentration gradients in a user-friendly and modular format that is compatible with conventional cell cultures and with tissue explant cultures. The device is simply inserted into a standard 6-well plate, where it hangs self-supported at a distance of ~250 μm above the cell culture surface. The gradient is created by small microflows from the device, through an integrated track-etched porous membrane, into the cell culture well. The microfluidic transwell can deliver stable, quantifiable gradients over a large area with extremely low fluid shear stress to dissociated cells or tissue explants cultured independently on the surface of a 6-well plate. We used finite-element modeling to describe the porous membrane flow and molecular transport and to predict gradients generated by the device. Using the device, we applied a gradient of the chemotactic peptide N-Formyl-Met-Leu-Phe (fMLP) to a large population of HL-60 cells (a neutrophil cell line) and directly observed the migration with time-lapse microscopy. On quantification of the chemotactic response with an automated tracking algorithm, we found 74% of the cells moving towards the gradient. Additionally, the modular design and low fluid shear stress made it possible to apply gradients of growth factors and second messengers to mouse retinal explant cultures. With a simplified interface and well-defined gradients, the microfluidic transwell device has potential for broad applications to gradient-sensing biology. PMID:24225908

Closed circuit TV system monitors welding operations

NASA Technical Reports Server (NTRS)

Gilman, M.

1967-01-01

TV camera system that has a special vidicon tube with a gradient density filter is used in remote monitoring of TIG welding of stainless steel. The welding operations involve complex assembly welding tools and skates in areas of limited accessibility.

Gradient Sensitivity to Within-Category Variation in Words and Syllables

ERIC Educational Resources Information Center

McMurray, Bob; Aslin, Richard N.; Tanenhaus, Michael K.; Spivey, Michael J.; Subik, Dana

2008-01-01

Five experiments monitored eye movements in phoneme and lexical identification tasks to examine the effect of within-category subphonetic variation on the perception of stop consonants. Experiment 1 demonstrated gradient effects along voice-onset time (VOT) continua made from natural speech, replicating results with synthetic speech (B. McMurray,…

Gradient Well-Formedness across the Morpheme Boundary

ERIC Educational Resources Information Center

Goldberg, Ariel M.

2010-01-01

Recent theories of phonology hold that phonotactic well-formedness may be gradient, with some legal structures being more well-formed than others. Linguistic and psycholinguistic research has demonstrated that "within" morphemes, speakers encode both categorical (*n/Onset) and gradient (st/Onset greater than sin/Onset) phonotactic restrictions.…

Method and means for a spatial and temporal probe for laser-generated plumes based on density gradients

DOEpatents

Yeung, Edward S.; Chen, Guoying

1990-05-01

A method and means for a spatial and temporal probe for laser generated plumes based on density gradients includes generation of a plume of vaporized material from a surface by an energy source. The probe laser beam is positioned so that the plume passes through the probe laser beam. Movement of the probe laser beam caused by refraction from the density gradient of the plume is monitored. Spatial and temporal information, correlated to one another, is then derived.

Data Validation Package August 2015 Groundwater Sampling at the Grand Junction, Colorado, Disposal Site October 2015

DOE Office of Scientific and Technical Information (OSTI.GOV)

Frazier, William; Baur, Gary

Sampling Period: August 4, 2015. The 1998 Interim Long-Term Surveillance Plan for the Cheney Disposal Site Near Grand Junction, Colorado, requires annual monitoring to assess the performance of the disposal cell. Monitoring wells 0731, 0732, and 0733 were sampled as specified in the plan. Sampling and analyses were conducted in accordance with Sampling and Analysis Plan for the U.S. Department of Energy Office of Legacy Management Sites (LMS/PRO/S04351, continually updated). The water level was measured at each sampled well. The water level in well 0733, located in the disposal cell, is lower than water levels in adjacent wells 0731 andmore » 0732, indicating a hydraulic gradient toward the disposal cell. Results from this sampling event were generally consistent with results from the past as shown in the attached concentration-versus-time graphs. There have been no large changes in contaminant concentration observed over the last several years with the following exception. The uranium concentration in well 0733 has been trending upward since 2003. High uranium concentrations are expected in this well because it is located in the disposal cell. The selenium concentrations observed in wells 0731 and 0732 are elevated when compared to the disposal cell 0733. Wells 0731 and 0732 are completed at the alluvium/Mancos contact; here, elevated selenium concentrations are expected due to contributions from the Mancos shale.« less

Joint Use of ERT, Tracer, and Numerical Techniques to Image Preferential Flow Paths in a Fractured Granite Aquifer

NASA Astrophysics Data System (ADS)

Sanaga, S.; Vijay, S.; Kbvn, P.; Peddinti, S. R.; P S L, S.

2017-12-01

Fractured geologic media poses formidable challenges to hydrogeologists due of the strenuous mapping of fracture-matrix system and quantification of flow and transport processes. In this research, we demonstrated the efficacy of tracer-ERT studies coupled with numerical simulations to delineate preferential flow paths in a fractured granite aquifer of Deccan traps in India. A series of natural gradient saline tracer experiments were conducted from a depth window of 18 to 22 m in an injection well located inside the IIT Hyderabad campus. Tracer migration was monitored in a time-lapse mode using two cross-sectional surface ERT profiles placed in the direction of flow gradient. Dynamic changes in sub-surface electrical properties inferred via resistivity anomalies were used to highlight preferential flow paths of the study area. ERT-derived tracer breakthrough curves were in agreement with geochemical sample measurements (R2=0.74). Fracture geometry and hydraulic properties derived from ERT and pumping tests were then used to evaluate two mathematical conceptualizations that are relevant to fractured aquifers. Results of numerical analysis conclude that a dual continuum model that combines matrix and fracture systems through a flow exchange term has outperformed equivalent continuum model in reproducing tracer concentrations at the monitoring wells (evident by decrease in RMSE from 199 mg/l to 65 mg/l). A sensitivity analysis of the model parameters reveals that spatial variability in hydraulic conductivity, local-scale dispersion, and flow exchange at fracture-matrix interface have a profound effect on model simulations. Keywords: saline tracer, ERT, fractured granite, groundwater, preferential flow, numerical simulation

Non-Destructive Monitoring of Charge-Discharge Cycles on Lithium Ion Batteries using 7Li Stray-Field Imaging

PubMed Central

Tang, Joel A.; Dugar, Sneha; Zhong, Guiming; Dalal, Naresh S.; Zheng, Jim P.; Yang, Yong; Fu, Riqiang

2013-01-01

Magnetic resonance imaging provides a noninvasive method for in situ monitoring of electrochemical processes involved in charge/discharge cycling of batteries. Determining how the electrochemical processes become irreversible, ultimately resulting in degraded battery performance, will aid in developing new battery materials and designing better batteries. Here we introduce the use of an alternative in situ diagnostic tool to monitor the electrochemical processes. Utilizing a very large field-gradient in the fringe field of a magnet, stray-field-imaging (STRAFI) technique significantly improves the image resolution. These STRAFI images enable the real time monitoring of the electrodes at a micron level. It is demonstrated by two prototype half-cells, graphite∥Li and LiFePO4∥Li, that the high-resolution 7Li STRAFI profiles allow one to visualize in situ Li-ions transfer between the electrodes during charge/discharge cyclings as well as the formation and changes of irreversible microstructures of the Li components, and particularly reveal a non-uniform Li-ion distribution in the graphite. PMID:24005580

A gradient model of vegetation and climate utilizing NOAA satellite imagery. Phase 1: Texas transect

NASA Technical Reports Server (NTRS)

Greegor, D. H.; Norwine, J.

1981-01-01

A new experimental climatological model/variable termed the sponge, a measure of moisture availability based on daily temperature maxima and minima and precipitation, is tested for potential biogeographic, ecological, and agro-climatological applications. Results, depicted in tabular and graphic from, suggest that, as a generalized climatic index, sponge's simplicity and sensitivity make particularly appropriate for trans-regional biogeographic studies (e.g., large-area and global vegetation monitoring). The feasibility of utilizing NOAA/AVHRR data for vegetation classification was investigated and a vegetation gradient model that utilizes sponge, and AVHRR pixel data (channels 1 and 2) were obtained for 12 locations. The normalized difference values for the AVHRR data when plotted against vegetation characteristics (biomass, net productivity, leaf area) and sponge values suggest that a multivariate gradient model incorporating AVHRR and sponge data may indeed be useful in global vegetation stratification and monitoring.

Final report : groundwater monitoring at Morrill, Kansas, in September 2005 and March 2006, with expansion of the monitoring network in January 2006.

DOE Office of Scientific and Technical Information (OSTI.GOV)

LaFreniere, L. M.; Environmental Science Division

2007-06-30

This document reports the results of groundwater monitoring in September 2005 and March 2006 at the grain storage facility formerly operated at Morrill, Kansas, by the Commodity Credit Corporation of the U.S. Department of Agriculture (CCC/USDA). These activities were the first and second twice yearly sampling events of the two-year monitoring program approved by the CCC/USDA and Kansas Department of Health and Environment (KDHE) project managers. The monitoring network sampled in September 2005 consisted of 9 monitoring wells (MW1S-MW5S and MW1D [installed in the mid 1990s] and MW6S-MW8S [installed in 2004]), plus 3 private wells (Isch, Rillinger, and Stone). Themore » groundwater samples collected in this first event were analyzed for volatile organic compounds (VOCs), dissolved hydrogen, and additional groundwater parameters to aid in evaluating the potential for reductive dechlorination processes. After the monitoring in September 2005, Argonne recommended expansion of the initial monitoring network. Previous sampling (August 2004) had already suggested that the initial network was inadequate to delineate the extent of the carbon tetrachloride plume. With the approval of the CCC/USDA and KDHE project managers, the monitoring network was expanded in January 2006 through the installation of 3 additional monitoring wells (MW9S-MW11S). Details of the monitoring well installations are reported in this document. The expanded monitoring network of 12 monitoring wells (MW1S-MW11S and MW1D) and 3 private wells (Isch, Rillinger, and Stone) was sampled in March 2006, the second monitoring event in the planned two-year program. Results of analyses for VOCs showed minor increases or decreases in contaminant levels at various locations but indicated that the leading edge of the contaminant plume is approaching the intermittent stream leading to Terrapin Creek. The groundwater samples collected in March 2006 were also analyzed for additional groundwater parameters to aid in the evaluation of the potential for reductive dechlorination processes. Preliminary screening of groundwater parameters provided inadequate evidence that reductive dechlorination of carbon tetrachloride is taking place at some locations on the former CCC/USDA property. Groundwater levels measured manually in October 2005, March 2006, and June 2006 were used to map the potentiometric surface at Morrill. The results were generally consistent with each other and with previous measurements, indicating a groundwater flow direction to the south-southeast from the former CCC/USDA facility. Data recorders installed in wells MW1S-MW8S in July 2004 are gathering long-term data on the groundwater elevation and gradient. Data downloaded in August 2004, March 2005, October 2005, and June 2006 indicate that two relatively upgradient wells near the former CCC/USDA facility responded distinctly to apparent rainfall/recharge events. In contrast, two downgradient wells south of the former facility showed virtually no response, probably because of the damping influence of the nearby surface drainages and shallow groundwater at their locations. The first two monitoring events of the planned two-year monitoring program for Morrill have demonstrated no clear pattern of changes in carbon tetrachloride concentrations, though the contaminated zone has expanded toward the intermittent stream. Argonne recommends that the monitoring program continue as approved and that surface water samples be collected in future monitoring events (September 2006, March 2007, and September 2007).« less

UAV lidar and hyperspectral fusion for forest monitoring in the southwestern USA

USGS Publications Warehouse

Sankey, Temuulen T.; Donager, Jonathon; McVay, Jason L.; Sankey, Joel B.

2017-01-01

Forest vegetation classification and structure measurements are fundamental steps for planning, monitoring, and evaluating large-scale forest changes including restoration treatments. High spatial and spectral resolution remote sensing data are critically needed to classify vegetation and measure their 3-dimensional (3D) canopy structure at the level of individual species. Here we test high-resolution lidar, hyperspectral, and multispectral data collected from unmanned aerial vehicles (UAV) and demonstrate a lidar-hyperspectral image fusion method in treated and control forests with varying tree density and canopy cover as well as in an ecotone environment to represent a gradient of vegetation and topography in northern Arizona, U.S.A. The fusion performs better (88% overall accuracy) than either data type alone, particularly for species with similar spectral signatures, but different canopy sizes. The lidar data provides estimates of individual tree height (R2 = 0.90; RMSE = 2.3 m) and crown diameter (R2 = 0.72; RMSE = 0.71 m) as well as total tree canopy cover (R2 = 0.87; RMSE = 9.5%) and tree density (R2 = 0.77; RMSE = 0.69 trees/cell) in 10 m cells across thin only, burn only, thin-and-burn, and control treatments, where tree cover and density ranged between 22 and 50% and 1–3.5 trees/cell, respectively. The lidar data also produces highly accurate digital elevation model (DEM) (R2 = 0.92; RMSE = 0.75 m). In comparison, 3D data derived from the multispectral data via structure-from-motion produced lower correlations with field-measured variables, especially in dense and structurally complex forests. The lidar, hyperspectral, and multispectral sensors, and the methods demonstrated here can be widely applied across a gradient of vegetation and topography for monitoring landscapes undergoing large-scale changes such as the forests in the southwestern U.S.A.

Simultaneous UHPLC-UV analysis of hydroxychloroquine, minocycline and doxycycline from serum samples for the therapeutic drug monitoring of Q fever and Whipple's disease.

PubMed

Armstrong, Nicholas; Richez, Magali; Raoult, Didier; Chabriere, Eric

2017-08-15

A fast UHPLC-UV method was developed for the simultaneous analysis of Hydroxychloroquine, Minocycline and Doxycycline drugs from 100μL of human serum samples. Serum samples were extracted by liquid-liquid extraction and injected into a phenyl hexyl reverse phase column. Compounds were separated using a mobile phase linear gradient and monitored by UV detection at 343nm. Chloroquine and Oxytetracycline were used as internal standards. Lower and upper limits of quantifications, as well as the other levels of calibration, were validated with acceptable accuracy (<15% deviation) and precision (<15% coefficient of variation) according to the European Medicines Agency guidelines. This new method enables cost and time reduction and was considered suitable for the clinical laboratory. It is the first published assay for the therapeutic drug monitoring of patients diagnosed with Q fever or Whipple's disease. Copyright © 2017 Elsevier B.V. All rights reserved.

Making sense of monitoring data using Jupyter Notebooks: a case study of dissolved oxygen dynamics across a fresh-estuarine gradient

NASA Astrophysics Data System (ADS)

Nelson, N.; Munoz-Carpena, R.

2016-12-01

In the presented exercise, students (advanced undergraduate-graduate) explore dissolved oxygen (DO) dynamics at three locations along a fresh-estuarine gradient of the Lower St. Johns River, FL (USA). Spatiotemporal DO trends along this gradient vary as a function of (1) tidal influence, and (2) biotic productivity (phytoplankton photosynthesis and community respiration). This combination of influences produces distinct DO behavior across each of the three hydrologically-connected sites. Through analysis of high frequency monitoring data, students are encouraged to think critically about the roles of physical and biological drivers of DO, and how the relative importance of these factors can vary among different locations within a single tidal waterbody. Data from each of the three locations along the river are downloaded with CUAHSI HydroClient, and analysis is performed with a Python-enabled Jupyter Notebook that has been specifically created for this assignment. Jupyter Notebooks include annotated code organized into blocks that are executed one-at-a-time; this format is amenable to classroom teaching, and provides an approachable introduction to Python for inexperienced coders. The outputs from each code block (i.e. graphs, tables) are produced within the Jupyter Notebook, thus allowing students to directly interact with the code. Expected student learning outcomes include increased spatial reasoning, as well as greater understanding of DO cycling, spatiotemporal variability in tidal systems, and challenges associated with collecting and evaluating large data sets. Specific technical learning outcomes include coding in Python for data management and analysis using Jupyter notebooks. This assignment and associated materials are open-access and available on the Science Education Resource Center website.

Subsurface temperatures and geothermal gradients on the North Slope, Alaska

USGS Publications Warehouse

Collett, Timothy S.; Bird, Kenneth J.; Magoon, Leslie B.

1989-01-01

Geothermal gradients as interpreted from a series of high-resolution stabilized well-bore-temperature surveys from 46 North Slope, Alaska, wells vary laterally and vertically throughout the near-surface sediment (0-2,000 m). The data from these surveys have been used in conjunction with depths of ice-bearing permafrost, as interpreted from 102 well logs, to project geothermal gradients within and below the ice-bearing permafrost sequence. The geothermal gradients calculated from the projected temperature profiles are similar to the geothermal gradients measured in the temperature surveys. Measured and projected geothermal gradients in the ice-bearing permafrost sequence range from 1.5??C/100m in the Prudhoe Bay area to 5.1??C/100m in the National Petroleum Reserve in Alaska (NPRA).

GBAS Ionospheric Anomaly Monitoring Based on a Two-Step Approach

PubMed Central

Zhao, Lin; Yang, Fuxin; Li, Liang; Ding, Jicheng; Zhao, Yuxin

2016-01-01

As one significant component of space environmental weather, the ionosphere has to be monitored using Global Positioning System (GPS) receivers for the Ground-Based Augmentation System (GBAS). This is because an ionospheric anomaly can pose a potential threat for GBAS to support safety-critical services. The traditional code-carrier divergence (CCD) methods, which have been widely used to detect the variants of the ionospheric gradient for GBAS, adopt a linear time-invariant low-pass filter to suppress the effect of high frequency noise on the detection of the ionospheric anomaly. However, there is a counterbalance between response time and estimation accuracy due to the fixed time constants. In order to release the limitation, a two-step approach (TSA) is proposed by integrating the cascaded linear time-invariant low-pass filters with the adaptive Kalman filter to detect the ionospheric gradient anomaly. The performance of the proposed method is tested by using simulated and real-world data, respectively. The simulation results show that the TSA can detect ionospheric gradient anomalies quickly, even when the noise is severer. Compared to the traditional CCD methods, the experiments from real-world GPS data indicate that the average estimation accuracy of the ionospheric gradient improves by more than 31.3%, and the average response time to the ionospheric gradient at a rate of 0.018 m/s improves by more than 59.3%, which demonstrates the ability of TSA to detect a small ionospheric gradient more rapidly. PMID:27240367

Mountain glaciers vs Ice sheet in Greenland - learning from a new monitoring site in West Greenland

NASA Astrophysics Data System (ADS)

Abermann, Jakob; van As, Dirk; Wacker, Stefan; Langley, Kirsty

2017-04-01

Only 5 out of the 20.000 peripheral glaciers and ice caps surrounding Greenland are currently monitored due to logistical challenges and despite their significance for sea level rise. Large spatial coast-to-icesheet mass and energy balance gradients limit simple upscaling methods from ice-sheet observations, which builds the motivation for this study. We present results from a new mass and energy balance time series at Qasigiannguit glacier (64°09'N; 51°21'W) in Southwest Greenland. Inter-annual variability is discussed and the surface energy balance over two summers is quantified and a ranking of the main drivers performed. We find that short-wave net radiation is by far the most dominant energy source during summer, followed by similar amounts of net longwave radiation and sensible heat, respectively. We then relate these observations to synchronous measurements at similar latitude on an outlet glacier of the ice sheet a mere 100 km away. We find very pronounced horizontal surface mass balance gradients, with generally more positive values closer to the coast. We conclude that despite minor differences of atmospheric parameters (i.e. humidity, radiation, and temperature) the main reason for the strongly different signal is a pronounced winter precipitation gradient that translates in a different duration of ice exposure and through that an albedo gradient. Modelled energy balance gradients converted into mass changes show good agreement to measured surface mass balance gradients and we explore a latitudinal signal of these findings.

43 CFR 3252.16 - How must I abandon a temperature gradient well?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How must I abandon a temperature gradient well? 3252.16 Section 3252.16 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.16 How must I abandon a temperature gradient well? (a...

43 CFR 3252.15 - When must I abandon a temperature gradient well?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false When must I abandon a temperature gradient well? 3252.15 Section 3252.15 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.15 When must I abandon a temperature gradient well? When...

43 CFR 3252.16 - How must I abandon a temperature gradient well?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How must I abandon a temperature gradient well? 3252.16 Section 3252.16 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.16 How must I abandon a temperature gradient well? (a...

43 CFR 3252.16 - How must I abandon a temperature gradient well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How must I abandon a temperature gradient well? 3252.16 Section 3252.16 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.16 How must I abandon a temperature gradient well? (a...

43 CFR 3252.15 - When must I abandon a temperature gradient well?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false When must I abandon a temperature gradient well? 3252.15 Section 3252.15 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.15 When must I abandon a temperature gradient well? When...

43 CFR 3252.16 - How must I abandon a temperature gradient well?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How must I abandon a temperature gradient well? 3252.16 Section 3252.16 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.16 How must I abandon a temperature gradient well? (a...

43 CFR 3252.15 - When must I abandon a temperature gradient well?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false When must I abandon a temperature gradient well? 3252.15 Section 3252.15 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.15 When must I abandon a temperature gradient well? When...

43 CFR 3252.15 - When must I abandon a temperature gradient well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false When must I abandon a temperature gradient well? 3252.15 Section 3252.15 Public Lands: Interior Regulations Relating to Public Lands (Continued... LEASING Conducting Exploration Operations § 3252.15 When must I abandon a temperature gradient well? When...

Establishing a gradient between partial pressure of arterial carbon dioxide and end-tidal carbon dioxide in patients with acute respiratory distress syndrome.

PubMed

Yousuf, Tariq; Brinton, Taylor; Murtaza, Ghulam; Wozniczka, Daniel; Ahmad, Khansa; Iskandar, Joy; Mehta, Raju; Keshmiri, Hesam; Hanif, Tabassum

2017-02-01

End-tidal carbon dioxide (ETCO 2 ) monitoring is useful in many situations. However, ETCO 2 monitoring is unreliable in patients with acute respiratory distress syndrome (ARDS) due to widespread lung inflammation. In our study, we attempt to establish the gradient between the arterial pressure of carbon dioxide (PaCO 2 ) and ETCO 2 in patients with ARDS, which we defined as the PaETCO 2 gradient. The main objective of the study was to establish a PaETCO 2 gradient in each severity of ARDS. We analyzed 35 patients with ARDS and a total of 88 arterial blood gases were included. PaCO 2 , PaO 2 /FiO 2 and ETCO 2 were measured. Patients were stratified into mild, moderate and severe ARDS as classified by the Berlin ARDS criteria. PaCO 2 and ETCO 2 were compared at each severity stratification. The mean PaCO 2 was 50.0, the mean ETCO 2 was 26.6 and the gradient among all samples was 23.24 (±12.02). The mean gradient for each severity is as follows: mild: 19.3 (±9.9), moderate: 27.9 (±13.2) and severe: 23.9 (±7.8). The difference between the PaETCO 2 gradient of the mild to moderate (p=0.001) and mild to severe groups (p=0.01) reached statistical significance. However, the difference between the moderate to severe groups did not reach statistical significance (p=0.48). We found the gradient between PaCO 2 and ETCO 2 in patients with ARDS is vast and tends to worsen with increasing severity of ARDS. This indicates that the gradient between the 2 may be used as an indicator of increasing severity of ARDS. Copyright © 2016 American Federation for Medical Research.

Phosphorus export across an urban to rural gradient in the Chesapeake Bay watershed

Treesearch

Shuiwang Duan; Sujay S. Kaushal; Peter Groffman; Lawrence E. Band; Kenneth Belt

2012-01-01

Watershed export of phosphorus (P) from anthropogenic sources has contributed to eutrophication in freshwater and coastal ecosystems. We explore impacts of watershed urbanization on the magnitude and export flow distribution of P along an urban-rural gradient in eight watersheds monitored as part of the Baltimore Ecosystem Study Long-Term Ecological Research site....

Tree Seedlings Establishment Across a Hydrologic Gradient in a Bottomland Restoration

Treesearch

Randall K. Kolka; Carl C. Trettin; E.A. Nelson; W.H. Conner

1998-01-01

Seedling establishment and survival on the Savannah River Site in South Carolina is being monitored as part of the Pen Branch Bottomland Restoration Project. Bottomland tree species were planted from 1993-1995 across a hydrologic gradient which encompasses the drier upper floodplain corridor, the lower floodplain corridor and the continuously inundated delta. Twelve...

Climatic and pollution influences on ecosystem processes in northern hardwood forests

Treesearch

Kurt S. Pregitzer; David D. Reed; Glenn D. Mroz; Andrew J. Burton; John A. Witter; Donald A. Zak

1996-01-01

The Michigan gradient study was established in 1987 to examine the effects of climate and atmospheric deposition on forest productivity and ecosystem processes in the Great Lakes region. Four intensively-monitored northern hardwood study sites are located along a climatic and pollutant gradient extending from southern lower Michigan to northwestern upper Michigan. The...

Geophysical monitoring of a field-scale biostimulation pilot project.

PubMed

Lane, John W; Day-Lewis, Frederick D; Casey, Clifton C

2006-01-01

The USGS conducted a geophysical investigation in support of a U.S. Naval Facilities Engineering Command, Southern Division field-scale biostimulation pilot project at Anoka County Riverfront Park (ACP), down-gradient of the Naval Industrial Reserve Ordnance Plant, Fridley, Minnesota. The goal of the pilot project is to evaluate subsurface injection of vegetable oil emulsion (VOE) to stimulate microbial degradation of chlorinated hydrocarbons. To monitor the emplacement and movement of the VOE and changes in water chemistry resulting from VOE dissolution and/or enhanced biological activity, the USGS acquired cross-hole radar zero-offset profiles, travel-time tomograms, and borehole geophysical logs during five site visits over 1.5 years. Analysis of pre- and postinjection data sets using petrophysical models developed to estimate VOE saturation and changes in total dissolved solids provides insights into the spatial and temporal distribution of VOE and ground water with altered chemistry. Radar slowness-difference tomograms and zero-offset slowness profiles indicate that the VOE remained close to the injection wells, whereas radar attenuation profiles and electromagnetic induction logs indicate that bulk electrical conductivity increased down-gradient of the injection zone, diagnostic of changing water chemistry. Geophysical logs indicate that some screened intervals were located above or below zones of elevated dissolved solids; hence, the geophysical data provide a broader context for interpretation of water samples and evaluation of the biostimulation effort. Our results include (1) demonstration of field and data analysis methods for geophysical monitoring of VOE biostimulation and (2) site-specific insights into the spatial and temporal distributions of VOE at the ACP.

Spatial gradients of OCPs in European butter--integrating environmental and exposure information.

PubMed

Weiss, Jana; Müller, Anne; Vives, Ingrid; Mariani, Giulio; Umlauf, Gunther

2013-05-01

The Stockholm Convention and the Global Monitoring Plan encourage the production of monitoring data to effectively evaluate the presence of the persistent organic pollutants (POPs) in all regions, in order to identify changes in levels over time, as well as to provide information on their regional and global environmental transport. Here, we report the first step of two to investigate whether butter is a feasible matrix to screen with the purpose to reflect regional ambient atmospheric air levels of POPs. The first step described here is to generate monitoring data; the second is to investigate the relationship between the two matrixes, i.e., POP concentrations in air and butter, which will be reported in another article published in this journal. Here, the 27 organochlorine pesticides listed under the Stockholm Convention have been analyzed in 75 butter samples from Europe. The general conclusions were as follows: Total organochlorine pesticide concentration is lower in butter from northern and central Europe. The spatial gradient of 1,1,1-trichloro-2,2-di(4-chlorophenyl)ethane and hexachlorocyclohexane is increasing in the eastern region of Europe (Romania, Bulgaria, and Ukraine), dieldrin towards France, and endosulfan levels were elevated on the Azores Island in the Atlantic Ocean. One butter sample from Romania exceeded the European Maximum Residue Limit value for lindane, but the other butter pesticide levels were all below the limit values. The dataset reported here can be used for the calibration of the air-grass-dairy products model, which would support the feasibility to use butter as biomonitor for measuring POP levels in ambient air.

Thermal Transmission through Existing Building Enclosures: Destructive Monitoring in Intermediate Layers versus Non-Destructive Monitoring with Sensors on Surfaces.

PubMed

Echarri, Víctor; Espinosa, Almudena; Rizo, Carlos

2017-12-08

Opaque enclosures of buildings play an essential role in the level of comfort experienced indoors and annual energy demand. The impact of solar radiation and thermal inertia of the materials that make up the multi-layer enclosures substantially modify thermal transmittance behaviour of the enclosures. This dynamic form of heat transfer, additionally affected by indoor HVAC systems, has a substantial effect on the parameters that define comfort. It also has an impact on energy demand within a daily cycle as well as throughout a one-year use cycle. This study describes the destructive monitoring of an existing block of flats located in Alicante. Once the enclosure was opened, sensors of temperature (PT100), air velocity, and relative humidity were located in the different layers of the enclosure, as well as in the interior and exterior surfaces. A pyranometer was also installed to measure solar radiation levels. A temperature data correction algorithm was drawn up to address irregularities produced in the enclosure. The algorithm was applied using a Raspberry Pi processor in the data collection system. The comparative results of temperature gradients versus non-destructive monitoring systems are presented, providing measures of the transmittance value, surface temperatures and indoor and outdoor air temperatures. This remote sensing system can be used in future studies to quantify and compare the energy savings of different enclosure construction solutions.

Thermal Transmission through Existing Building Enclosures: Destructive Monitoring in Intermediate Layers versus Non-Destructive Monitoring with Sensors on Surfaces

PubMed Central

Echarri, Víctor; Espinosa, Almudena; Rizo, Carlos

2017-01-01

Opaque enclosures of buildings play an essential role in the level of comfort experienced indoors and annual energy demand. The impact of solar radiation and thermal inertia of the materials that make up the multi-layer enclosures substantially modify thermal transmittance behaviour of the enclosures. This dynamic form of heat transfer, additionally affected by indoor HVAC systems, has a substantial effect on the parameters that define comfort. It also has an impact on energy demand within a daily cycle as well as throughout a one-year use cycle. This study describes the destructive monitoring of an existing block of flats located in Alicante. Once the enclosure was opened, sensors of temperature (PT100), air velocity, and relative humidity were located in the different layers of the enclosure, as well as in the interior and exterior surfaces. A pyranometer was also installed to measure solar radiation levels. A temperature data correction algorithm was drawn up to address irregularities produced in the enclosure. The algorithm was applied using a Raspberry Pi processor in the data collection system. The comparative results of temperature gradients versus non-destructive monitoring systems are presented, providing measures of the transmittance value, surface temperatures and indoor and outdoor air temperatures. This remote sensing system can be used in future studies to quantify and compare the energy savings of different enclosure construction solutions. PMID:29292781

Subsurface temperatures and geothermal gradients on the north slope of Alaska

USGS Publications Warehouse

Collett, T.S.; Bird, K.J.; Magoon, L.B.

1993-01-01

On the North Slope of Alaska, geothermal gradient data are available from high-resolution, equilibrated well-bore surveys and from estimates based on well-log identification of the base of ice-bearing permafrost. A total of 46 North Slope wells, considered to be in or near thermal equilibrium, have been surveyed with high-resolution temperatures devices and geothermal gradients can be interpreted directly from these recorded temperature profiles. To augment the limited North Slope temperature data base, a new method of evaluating local geothermal gradients has been developed. In this method, a series of well-log picks for the base of the ice-bearing permafrost from 102 wells have been used, along with regional temperature constants derived from the high-resolution stabilized well-bore temperature surveys, to project geothermal gradients. Geothermal gradients calculated from the high-resolution temperature surveys generally agree with those projected from known ice-bearing permafrost depths over most of the North Slope. Values in the ice-bearing permafrost range from ??? 1.5??C 100 m in the Prudhoe Bay area to ??? 4.5??C 100 m in the east-central portion of the National Petroleum Reserve in Alaska. Geothermal gradients below the ice-bearing permafrost sequence range from ??? 1.6??C 100 m to ??? 5.2??C 100 m. ?? 1993.

Nonlinear Analyte Concentration Gradients for One-Step Kinetic Analysis Employing Optical Microring Resonators

PubMed Central

Marty, Michael T.; Kuhnline Sloan, Courtney D.; Bailey, Ryan C.; Sligar, Stephen G.

2012-01-01

Conventional methods to probe the binding kinetics of macromolecules at biosensor surfaces employ a stepwise titration of analyte concentrations and measure the association and dissociation to the immobilized ligand at each concentration level. It has previously been shown that kinetic rates can be measured in a single step by monitoring binding as the analyte concentration increases over time in a linear gradient. We report here the application of nonlinear analyte concentration gradients for determining kinetic rates and equilibrium binding affinities in a single experiment. A versatile nonlinear gradient maker is presented, which is easily applied to microfluidic systems. Simulations validate that accurate kinetic rates can be extracted for a wide range of association and dissociation rates, gradient slopes and curvatures, and with models for mass transport. The nonlinear analyte gradient method is demonstrated with a silicon photonic microring resonator platform to measure prostate specific antigen-antibody binding kinetics. PMID:22686186

Nonlinear analyte concentration gradients for one-step kinetic analysis employing optical microring resonators.

PubMed

Marty, Michael T; Sloan, Courtney D Kuhnline; Bailey, Ryan C; Sligar, Stephen G

2012-07-03

Conventional methods to probe the binding kinetics of macromolecules at biosensor surfaces employ a stepwise titration of analyte concentrations and measure the association and dissociation to the immobilized ligand at each concentration level. It has previously been shown that kinetic rates can be measured in a single step by monitoring binding as the analyte concentration increases over time in a linear gradient. We report here the application of nonlinear analyte concentration gradients for determining kinetic rates and equilibrium binding affinities in a single experiment. A versatile nonlinear gradient maker is presented, which is easily applied to microfluidic systems. Simulations validate that accurate kinetic rates can be extracted for a wide range of association and dissociation rates, gradient slopes, and curvatures, and with models for mass transport. The nonlinear analyte gradient method is demonstrated with a silicon photonic microring resonator platform to measure prostate specific antigen-antibody binding kinetics.

43 CFR 3252.12 - How deep may I drill a temperature gradient well?

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false How deep may I drill a temperature... RESOURCE LEASING Conducting Exploration Operations § 3252.12 How deep may I drill a temperature gradient well? (a) You may drill a temperature gradient well to any depth that we approve in your exploration...

43 CFR 3252.12 - How deep may I drill a temperature gradient well?

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false How deep may I drill a temperature... RESOURCE LEASING Conducting Exploration Operations § 3252.12 How deep may I drill a temperature gradient well? (a) You may drill a temperature gradient well to any depth that we approve in your exploration...

43 CFR 3252.12 - How deep may I drill a temperature gradient well?

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false How deep may I drill a temperature... RESOURCE LEASING Conducting Exploration Operations § 3252.12 How deep may I drill a temperature gradient well? (a) You may drill a temperature gradient well to any depth that we approve in your exploration...

43 CFR 3252.12 - How deep may I drill a temperature gradient well?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false How deep may I drill a temperature... RESOURCE LEASING Conducting Exploration Operations § 3252.12 How deep may I drill a temperature gradient well? (a) You may drill a temperature gradient well to any depth that we approve in your exploration...

Surfactant-enhanced remediation of a trichloroethene-contaminated aquifer. 2. Transport of TCE

USGS Publications Warehouse

Sahoo, D.; Smith, J.A.; Imbrigiotta, T.E.; Mclellan, H.M.

1998-01-01

Field studies were conducted under an induced gradient in a trichloroethene (TCE)-contaminated aquifer at Picatinny Arsenal, NJ, to study (a) the rate-limited desorption of TCE from aquifer sediments to water and (b) the effect of a surfactant (Triton X-100) on the desorption and transport of TCE. Clean water was injected into the contaminated aquifer for 206 day. Triton X-100 was added for a 36-day period (days 36-71 from the start of clean water injection). The effect of Triton X-100 on the desorption and transport of TCE in the field was examined by observing the concentrations of these two solutes in four monitoring wells 3-9 m from the injection wells. These data show a small but discernible increase in the TCE concentration in two of the wells corresponding approximately to the time when surfactant reaches the wells; in the other two monitoring wells, the increase in TCE concentration is negligible. A solute transport model that assumes local sorption equilibrium and used a laboratory-derived distribution coefficient could not adequately describe TCE desorption and transport observed in the aquifer. Two model formulations that accounted for rate-limited sorption - two-site and multisite models - fit the data well. TCE concentrations after surfactant injection were underpredicted by the models unless mass transfer rate was increased to account for the effect of surfactant on the rate of TCE desorption. The concentration data from the two wells and the model analysis suggest that the rate of TCE desorption is increased (by approximately 30%) as a result of Triton X-100 injection.Field studies were conducted under an induced gradient in a trichloroethene (TCE)-contaminated aquifer at Picatinny Arsenal, NJ, to study (a) the rate-limited desorption of TCE from aquifer sediments to water and (b) the effect of a surfactant (Triton X-100) on the desorption and transport of TCE. Clean water was injected into the contaminated aquifer for 206 day. Triton X-100 was added for a 36-day period (days 36-71 from the start of clean water injection). The effect of Triton X-100 on the desorption and transport of TCE in the field was examined by observing the concentrations of these two solutes in four monitoring wells 3-9 m from the injection wells. These data show a small but discernible increase in the TCE concentration in two of the wells corresponding approximately to the time when surfactant reaches the wells; in the other two monitoring wells, the increase in TCE concentration is negligible. A solute transport model that assumes local sorption equilibrium and used a laboratory-derived distribution coefficient could not adequately describe TCE desorption and transport observed in the aquifer. Two model formulations that accounted for rate-limited sorption - two-site and multisite models - fit the data well. TCE concentrations after surfactant injection were underpredicted by the models unless mass transfer rate was increased to account for the effect of surfactant on the rate of TCE desorption. The concentration data from the two wells and the model analysis suggest that the rate of TCE desorption is increased (by approximately 30%) as a result of Triton X-100 injection.

Method and means for a spatial and temporal probe for laser-generated plumes based on density gradients

DOEpatents

Yeung, E.S.; Chen, G.

1990-05-01

A method and means are disclosed for a spatial and temporal probe for laser generated plumes based on density gradients includes generation of a plume of vaporized material from a surface by an energy source. The probe laser beam is positioned so that the plume passes through the probe laser beam. Movement of the probe laser beam caused by refraction from the density gradient of the plume is monitored. Spatial and temporal information, correlated to one another, is then derived. 15 figs.

Towards stressor-specific macroinvertebrate indices: Which traits and taxonomic groups are associated with vulnerable and tolerant taxa?

PubMed

Berger, Elisabeth; Haase, Peter; Schäfer, Ralf B; Sundermann, Andrea

2018-04-01

Monitoring of macroinvertebrate communities is frequently used to define the ecological health status of rivers. Ideally, biomonitoring should also give an indication on the major stressors acting on the macroinvertebrate communities supporting the selection of appropriate management measures. However, most indices are affected by more than one stressor. Biological traits (e.g. size, generation time, reproduction) could potentially lead to more stressor-specific indices. However, such an approach has rarely been tested. In this study we classify 324 macroinvertebrate taxa as vulnerable (decreasing abundances) or tolerant (increasing abundances) along 21 environmental gradients (i.e. nutrients, major ions, oxygen and micropollutants) from 422 monitoring sites in Germany using Threshold Indicator Taxa Analysis (TITAN). Subsequently, we investigate which biological traits and taxonomic groups are associated with taxa classified as vulnerable or tolerant with regard to specific gradients. The response of most taxa towards different gradients was similar and especially high for correlated gradients. Traits associated with vulnerable taxa across most gradients included: larval aquatic life stages, isolated cemented eggs, reproductive cycle per year <1, scrapers, aerial and aquatic active dispersal and plastron respiration. Traits associated with tolerant taxa included: adult aquatic life stages, polyvoltinism, ovoviviparity or egg clutches in vegetation, food preference for dead animals or living microinvertebrates, substrate preference for macrophytes, microphytes, silt or mud and a body size >2-4cm. Our results question whether stressor-specific indices based on macroinvertebrate assemblages can be achieved using single traits, because we observed that similar taxa responded to different gradients and also similar traits were associated with vulnerable and tolerant taxa across a variety of water quality gradients. Future studies should examine whether combinations of traits focusing on specific taxonomic groups achieve higher stressor specificity. Copyright © 2017 Elsevier B.V. All rights reserved.

Understanding High Temperature Gradients in the Buckman Well Field, Santa Fe County, New Mexico

NASA Astrophysics Data System (ADS)

Folsom, M.; Gulvin, C. J.; Tamakloe, F. M.; Yauk, K.; Kelley, S.; Frost, J.; Jiracek, G. R.

2014-12-01

We propose a conceptual model to explain elevated thermal gradients, localized laterally over a few 100 m, discovered during the SAGE program in 2013 and confirmed in 2014 at the Buckman water well field in the Española Basin of north central New Mexico. The anomalous gradients of temperature with depth, dT/dz, exceed 70 ºC/km and are found in three shallow (< 100 m-deep) USGS monitoring wells close to the Rio Grande. A temperature increase of only 3 - 4 ºC at ~100 m depth would elevate the regional temperature value enough to yield the anomalous dT/dz values in the upper ~100 m. The coincidence of a 25 km2 region of InSAR-confirmed subsidence with the locally anomalous dT/dz region suggests a way to achieve a higher temperature at ~ 100 m depth. The mechanism is an isothermal release of warmer water from ~ 200 m depth along a fissure or reactivated fault. A fourth well, 290 m away, has a temperature gradient of only 33ºC/km in the upper 100 m and a distinctly different geochemical profile, suggesting aquifer compartmentalization and possible faulting close to the anomaly. In 2001 a 800 m-long surface scarp with up to 0.2 m offset appeared 2 km to the east in response to over-pumping that depressed the groundwater table by over 100 m. Such drawdown is expected to have 2 - 5 m of compaction with attendant movement along faults or fissures. This could allow groundwater to be released upward isothermally until encountering an unbreached aquitard where it would establish an elevated thermal boundary. Besides the local thermal anomaly, we have temperature-logged deeper water wells in the area. These and other measurements have been used to construct cross-sections of isotherms across the Española Basin along the groundwater flow units (GFUs). This allows comparison of the local thermal anomaly with classic, regional, basin hydrological models. For example, the fully-screened Skillet well, 2.3 km from the anomaly, shows a classic concave down dT/dz form indicating upwelling water. This is consistent with the regional hydraulic head and historical accounts of artesian wells pre-dating Buckman pumping. We quantified the upwelling by Péclet number analysis to be 0.076 - 0.11 m/yr. Numerical modeling using the TOUGH2 computer code is proceeding to further understand regional and local subsurface groundwater flow patterns and dT/dz values.

Denitrification in sediments from the hyporheic zone adjacent to a small forested stream

USGS Publications Warehouse

Duff, J.H.; Triska, F.J.

1990-01-01

Denitrifying potentials increased with increasing distance from the stream channel. Dissolved oxygen was 100% of the concentration expected in equilibrium with the atmosphere in water obtained from monitoring wells immediately adjacent to the stream but was as low as 7% of the expected value in water 11.4 m inland. Both nitrate and dissolved organic carbon decreased over summer in wells at the base of the alder-forested slope. A 48-h injection of nitrate-amended stream water into hyporheic water 8.4 m inland stimulated nitrous oxide production in the presence of acetylene. Nitrous oxide was generated as nitrate and acetylene were co-transported to a well 13 m down-gradient. Acetylene-block experiments coupled with the chemistry data suggest that denitrification can modify the chemistry of water during passage through the hyporheic zone. -from Authors

One-dimensional transient finite difference model of an operational salinity gradient solar pond

NASA Technical Reports Server (NTRS)

Hicks, Michael C.; Golding, Peter

1992-01-01

This paper describes the modeling approach used to simulate the transient behavior of a salinity gradient solar pond. A system of finite difference equations are used to generate the time dependent temperature and salinity profiles within the pond. The stability of the pond, as determined by the capacity of the resulting salinity profile to suppress thermal convection within the primary gradient region of the pond, is continually monitored and when necessary adjustments are made to the thickness of the gradient zone. Results of the model are then compared to measurements taken during two representative seasonal periods at the University of Texas at El Paso's (UTEP's) research solar pond.

Hydrogeology and water quality of sand and gravel aquifers in McHenry County, Illinois, 2009-14, and comparison to conditions in 1979

USGS Publications Warehouse

Gahala, Amy M.

2017-10-26

Baseline conditions for the sand and gravel aquifers (groundwater) in McHenry County, Illinois, were assessed using data from a countywide network of 44 monitoring wells collecting continuous water-level data from 2009–14. In 2010, water-quality data were collected from 41 of the monitoring wells, along with five additional monitoring wells available from the U.S. Geological Survey National Water Quality Assessment Program. Periodic water-quality data were collected from 2010–14 from selected monitoring wells. The continuous water-level data were used to identify the natural and anthropogenic factors that influenced the water levels at each well. The water-level responses to natural influences such as precipitation, seasonal and annual variations, barometric pressure, and geology, and to anthropogenic influences such as pumping were used to determine (1) likely hydrogeologic setting (degree of aquifer confinement and interconnections) that, in part, are related to lithostratigraphy; and (2) areas of recharge and discharge related to vertical flow directions. Water-level trends generally were determined from the 6 years of data collection (2009–14) to infer effects of weather variability (drought) on recharge.Precipitation adds an estimated 2.4 inches per year of recharge to the aquifer. Some of this recharge is subsequently discharged to streams and some is discharged to supply wells. A few areas in the eastern half of the county had higher average recharge rates, indicating a need for adequate protection of these recharge areas. Downward vertical flow gradients in upland areas indicate that recharge to the confined aquifer units occurs near upland areas. Upward vertical flow gradients in lowland areas indicate discharge at locations of surface water and groundwater interaction (wetlands, ponds, and streams).Monitoring wells were sampled for major and minor ions, metals, and nutrients and a subset of wells was sampled for trace elements, dissolved gases, pesticides, and volatile organic compounds. The results were compared to health‑based and aesthetically based standards, which include the U.S. Environmental Protection Agency Maximum Contaminant Level (EPA MCL), and EPA Secondary Maximum Contaminant Levels (SMCL), as well as EPA Health-based Standards Drinking Water Advisories. Health‑based standards were exceeded for arsenic in 22 percent, sodium in 20 percent, and nitrates in 2 percent of the monitoring wells sampled. Aesthetically based standards were exceeded for total dissolved solids in 33 percent, chloride in 11 percent, iron in 85 percent, and manganese in 30 percent of the wells sampled. Many of these same constituents, such as arsenic, iron, and manganese, are naturally occurring but become elevated in areas that have anoxic, mixed, and suboxic conditions. Some areas of potential vulnerability to anthropogenic-sourced constituents in the sand and gravel aquifers were evidenced by trace amounts of volatile organic compounds and pesticides detected in water-quality samples from shallow wells (total depth less of than 46 feet below land surface) near urban settings, and by the detection of elevated major ions (chloride, sodium, magnesium, and calcium) associated, in part, with road-salt applications. Source analysis for chloride indicates mixtures of road salt, water softeners, and sewage.Continuously measured specific conductance values were used as a surrogate for continuously measured chloride concentrations in the groundwater. The estimated chloride concentrations generally were highest in spring and lowest in summer, and occasionally peak during spring melt. Overall, the range of concentrations varied depending on the local thickness and hydraulic conductivity of the aquifer.Water levels and water quality from the countywide groundwater monitoring network were compared to water levels and water-quality results in 1979 from a previous U.S. Geological Survey study. Potentiometric surface maps show areas with inferred decreases of water levels near the southern and southeastern areas of McHenry County. Significant increases were noted for total dissolved solids and specific conductance. Chloride concentrations increased as much as 521 percent in three of six wells resampled in 2015 from the previous study.

Runoff and recharge processes under a strong semi-arid climatic gradient

NASA Astrophysics Data System (ADS)

Ries, F.; Lange, J.; Sauter, M.; Schmidt, S.

2012-04-01

Hydrological processes in semi-arid environments are highly dynamic. In the eastern slopes of the West Bank these dynamics are even intensified due to the predominant karst morphology, the strong climatic gradient (150-700 mm mean annual precipitation) and the small-scale variability of land use, topography and soil cover. The region is characterized by a scarcity in water resources and a high population growth. Therefore detailed information about the temporal and spatial distribution, amount and variability of available water resources is required. Providing this information by the use of hydrological models is challenging, because available data are extremely limited. From 2007 on, the research area of Wadi Auja, northeast of Jerusalem, has been instrumented with a dense monitoring network. Rainfall distribution and climatic parameters as well as the hydrological reaction of the system along the strong semi-arid climatic gradient are measured on the plot (soil moisture), hillslope (runoff generation) and catchment scale (spring discharge, groundwater level, flood runoff). First data from soil moisture plots situated along the climatic gradient are presented. They allow insights into physical properties of the soil layer and its impact on runoff and recharge processes under different climatic conditions. From continuous soil moisture profiles, soil water balances are calculated for singe events and entire seasons. These data will be used to parameterize the distributed hydrological model TRAIN-ZIN, which has been successfully applied in several studies in the Jordan River Basin.

MR-based detection of individual histotripsy bubble clouds formed in tissues and phantoms.

PubMed

Allen, Steven P; Hernandez-Garcia, Luis; Cain, Charles A; Hall, Timothy L

2016-11-01

To demonstrate that MR sequences can detect individual histotripsy bubble clouds formed inside intact tissues. A line-scan and an EPI sequence were sensitized to histotripsy by inserting a bipolar gradient whose lobes bracketed the lifespan of a histotripsy bubble cloud. Using a 7 Tesla, small-bore scanner, these sequences monitored histotripsy clouds formed in an agar phantom and in vitro porcine liver and brain. The bipolar gradients were adjusted to apply phase with k-space frequencies of 10, 300 or 400 cm -1 . Acoustic pressure amplitude was also varied. Cavitation was simultaneously monitored using a passive cavitation detection system. Each image captured local signal loss specific to an individual bubble cloud. In the agar phantom, this signal loss appeared only when the transducer output exceeded the cavitation threshold pressure. In tissues, bubble clouds were immediately detected when the gradients created phase with k-space frequencies of 300 and 400 cm -1 . When the gradients created phase with a k-space frequency of 10 cm -1 , individual bubble clouds were not detectable until many acoustic pulses had been applied to the tissue. Cavitation-sensitive MR-sequences can detect single histotripsy bubble clouds formed in biologic tissue. Detection is influenced by the sensitizing gradients and treatment history. Magn Reson Med 76:1486-1493, 2016. © 2015 International Society for Magnetic Resonance in Medicine. © 2015 International Society for Magnetic Resonance in Medicine.

Evaluating area-based socioeconomic status indicators for monitoring disparities within health care systems: results from a primary care network.

PubMed

Berkowitz, Seth A; Traore, Carine Y; Singer, Daniel E; Atlas, Steven J

2015-04-01

To determine which area-based socioeconomic status (SES) indicator is best suited to monitor health care disparities from a delivery system perspective. 142,659 adults seen in a primary care network from January 1, 2009 to December 31, 2011. Cross-sectional, comparing associations between area-based SES indicators and patient outcomes. Address data were geocoded to construct area-based SES indicators at block group (BG), census tract (CT), and ZIP code (ZIP) levels. Data on health outcomes were abstracted from electronic records. Relative indices of inequality (RIIs) were calculated to quantify disparities detected by area-based SES indicators and compared to RIIs from self-reported educational attainment. ZIP indicators had less missing data than BG or CT indicators (p < .0001). Area-based SES indicators were strongly associated with self-report educational attainment (p < .0001). ZIP, BG, and CT indicators all detected expected SES gradients in health outcomes similarly. Single-item, cut point defined indicators performed as well as multidimensional indices and quantile indicators. Area-based SES indicators detected health outcome differences well and may be useful for monitoring disparities within health care systems. Our preferred indicator was ZIP-level median household income or percent poverty, using cut points. © Health Research and Educational Trust.

The association between the pulse pressure gradient at the cranio-cervical junction derived from phase-contrast magnetic resonance imaging and invasively measured pulsatile intracranial pressure in symptomatic patients with Chiari malformation type 1.

PubMed

Frič, Radek; Lindstrøm, Erika Kristina; Ringstad, Geir Andre; Mardal, Kent-André; Eide, Per Kristian

2016-12-01

In symptomatic Chiari malformation type 1 (CMI), impaired intracranial compliance (ICC) is associated with an increased cranio-spinal pulsatile pressure gradient. Phase-contrast magnetic resonance imaging (MRI) represents a non-invasive modality for the assessment of the pulse pressure gradient at the cranio-cervical junction (CCJ). We wished to explore how the MRI-derived pulse pressure gradient (MRI-dP) compares with invasively measured pulsatile intracranial pressure (ICP) in CMI, and with healthy controls. From phase-contrast MRI of CMI patients and healthy controls, we computed cerebrospinal fluid (CSF) flow velocities and MRI-dP at the CCJ. We assessed bidirectional flow and compared the flow between the anterior and the posterior subarachnoid space at the CCJ. We computed total intracranial volume (ICV), ventricular CSF volume (VV), and posterior cranial fossa volume (PCFV). We analyzed the static and pulsatile ICP scores from overnight monitoring in CMI patients. Five CMI patients and four healthy subjects were included. The CMI group had a significantly larger extent of tonsillar ectopia, smaller PCFV, and a smaller area of CSF in the FM. The pulsatile ICP (mean ICP wave amplitude, MWA) was abnormally increased in 4/5 CMI patients and correlated positively with MRI-dP. However, the MRI-dP as well as the CSF flow velocities did not differ significantly between CMI and healthy subjects. Moreover, bidirectional flow was observed in both CMI as well as healthy subjects, with no significant difference. In symptomatic CMI patients, we found a significant association between the pulse pressure gradient at the CCJ derived from phase-contrast MRI and the pulsatile ICP (MWA) measured invasively. However, the MRI-dP was close to identical in CMI patients and healthy subjects. Moreover, the CSF flow velocities at the CCJ and the occurrence of bidirectional flow were not different in CMI patients and healthy individuals. Further studies are required to determine the diagnostic role of phase-contrast MRI in CMI patients.

Molecular Probe Fluorescence Monitoring of Polymerization

NASA Technical Reports Server (NTRS)

Bunton, Patrick

2002-01-01

This project investigated the feasibility of using fluorescence spectroscopy to determine viscosity of polymer/monomer in support of Transient Interfacial Phenomena in Miscible Polymer Systems (TIPMPS). This project will attempt to measure gradient induced flow at a miscible interface during and / or after in-flight polymerization of dodecyl acrylate (lauryl acrylate). Concentration and temperature gradients will be intentionally introduced during polymerization and the resultant fluid flow determined by Particle Imaging Velocimetry (PIV). This report describes an investigation of the feasibility of using fluorescence of a probe molecule to monitor viscosity and/or concentration during and after polymerization. The probe used was pyrene which has been shown to be sensitive to its local environment in methyl methacrylate.

Leaf reflectance variation along a vertical crown gradient of two deciduous tree species in a Belgian industrial habitat.

PubMed

Khavaninzadeh, Ali Reza; Veroustraete, Frank; Van Wittenberghe, Shari; Verrelst, Jochem; Samson, Roeland

2015-09-01

The reflectometry of leaf asymmetry is a novel approach in the bio-monitoring of tree health in urban or industrial habitats. Leaf asymmetry responds to the degree of environmental pollution and reflects structural changes in a leaf due to environmental pollution. This paper describes the boundary conditions to scale up from leaf to canopy level reflectance, by describing the variability of adaxial and abaxial leaf reflectance, hence leaf asymmetry, along the crown height gradients of two tree species. Our findings open a research pathway towards bio-monitoring based on the airborne remote sensing of tree canopies and their leaf asymmetric properties. Copyright © 2015 Elsevier Ltd. All rights reserved.

Reconnaissance of water quality at four swine farms in Jackson County, Florida, 1993

USGS Publications Warehouse

Collins, J.J.

1996-01-01

The quality of ground water on four typical swine farms in Jackson County, Florida, was studied by analyzing water samples from wastewater lagoons, monitoring wells, and supply wells. Water samples were collected quarterly for 1 year and analyzed for the following dissolved species: nitrate, nitrite, ammonium nitrogen, phosphorus, potassium, sulfate, chloride, calcium, magnesium, fluoride, total ammonium plus organic nitrogen, total phosphorus, alkalinity, carbonate, and bicarbonate. Additionally, the following field constituents were determined in the water samples: temperature, specific conductance, pH, dissolved oxygen, and fecal streptococcus and fecal coliform bacteria. Chemical changes in swine waste as it leaches and migrates through the saturated zone were examined by comparing median values and ranges of water- quality data from farm wastewater in lagoons, shallow pond, shallow monitoring wells, and deeper farm supply wells. The effects of hydrogeologic settings and swine farmland uses on shallow ground-water quality were examined by comparing the shallow ground-water-quality data set with the results of the chemical analyses of water from the Upper Floridan aquifer, and to land uses adjacent to the monitoring wells. Substantial differences occur between the quality of diluted swine waste in the wastewater lagoons, and that of the water quality found in the shallow pond, and the ground water frm all but two of the monitoring wells of the four swine farms. The liquid from the wastewater lagoons and ground water from two wells adjacent to and down the regional gradient from a lagoon on one site, have relatively high values for the following properties and constituents: specific conductance, dissolved ammonia nitrogen, dissolved potassium, and dissolved chloride. Ground water from all other monitoring wells and farm supply wells and the surface water pond, have relatively much lower values for the same properties and constituents. To determine the relation between land uses and ground-water quality on the four swine farms, ground-water-quality data were divided according to the following land uses: confined operations in which swine are kept in houses and not allowed to roam freely, and unconfined operations in which swine are allowed to roam freely in determined areas. Confined operations had lagoons to receive the diluted swine wastes washed from the houses.

COMPLEMENTARY MONITORING DESIGNS TO DOCUMENT REGIONAL GRADIENTS, AND TEMPORTAL VARIATIONS OF DISSOLOVED OXYGEN IN ESTUARINE WATERS

EPA Science Inventory

The U.S. EPA National Coastal Assessment program is designed to address two broad questions: 1) what are the conditions of estuarine resources, how are they changing, and what causes those changes; 2) which monitoring designs, indicators, and protocols are appropriate for assessi...

Fluid Exchange Across the Seafloor of the Continental Shelf in the South Atlantic Bight

NASA Astrophysics Data System (ADS)

White, S. M.; Wilson, A. M.; Moore, W. S.; Smoak, E. A.; George, C.

2014-12-01

Increasing evidence suggests that saline submarine groundwater discharges from the seafloor in volumes that rival river discharge, but this discharge occurs far from shore, spread regionally across the continental shelves. The very limited observational data suggest that saline discharge occurs via long-term regional flow systems and rapid flushing of porewaters from sandy sediment during storm events. This study aims to overcome the paucity of available observational constraints on characterizing regional-scale fluid exchange on passive margin continental shelves. We are developing a detailed hydrostratigraphic framework based on 200 km of CHIRP seismic lines 5-20 km offshore from Charleston, SC and 13 sediment cores up to 6.5 m long. This survey revealed varying thicknesses (0-15 m) of sediment overlying Cretaceous limestone basement, and a filled paleochannel fluvial system. We have installed 3 sets of nested wells and an additional 10 temperature-gradient arrays to observe a wide variety of environments across the shelf. The wells and thermal arrays have been recently installed in the upper 5 m of the sediment, to allow monitoring of pressure and temperature. The wells will also be sampled for Ra tracers and nutrient concentrations. The combination of wells and survey data will allow us to estimate rates of submarine groundwater discharge via hydraulic gradients and by using heat and geochemical tracers. We have developed a numerical model to invert thermal data to estimate both long-term regional groundwater flow and rapid flushing associated with storm events.

Drop casting of stiffness gradients for chip integration into stretchable substrates

NASA Astrophysics Data System (ADS)

Naserifar, Naser; LeDuc, Philip R.; Fedder, Gary K.

2017-04-01

Stretchable electronics have demonstrated promise within unobtrusive wearable systems in areas such as health monitoring and medical therapy. One significant question is whether it is more advantageous to develop holistic stretchable electronics or to integrate mature CMOS into stretchable electronic substrates where the CMOS process is separated from the mechanical processing steps. A major limitation with integrating CMOS is the dissimilar interface between the soft stretchable and hard CMOS materials. To address this, we developed an approach to pattern an elastomeric polymer layer with spatially varying mechanical properties around CMOS electronics to create a controllable material stiffness gradient. Our experimental approach reveals that modifying the interfaces can increase the strain failure threshold up to 30% and subsequently decreases delamination. The stiffness gradient in the polymer layer provides a safe region for electronic chips to function under a substrate tensile strain up to 150%. These results will have impacts in diverse applications including skin sensors and wearable health monitoring systems.

Variation in the health and biochemical condition of the coral Acropora tenuis along two water quality gradients on the Great Barrier Reef, Australia.

PubMed

Rocker, Melissa M; Francis, David S; Fabricius, Katharina E; Willis, Bette L; Bay, Line K

2017-06-30

This study explores how plasticity in biochemical attributes, used as indicators of health and condition, enables the coral Acropora tenuis to respond to differing water quality regimes in inshore regions of the Great Barrier Reef. Health attributes were monitored along a strong and weak water quality gradient, each with three reefs at increasing distances from a major river source. Attributes differed significantly only along the strong gradient; corals grew fastest, had the least dense skeletons, highest symbiont densities and highest lipid concentrations closest to the river mouth, where water quality was poorest. High nutrient and particulate loads were only detrimental to skeletal density, which decreased as linear extension increased, highlighting a trade-off. Our study underscores the importance of assessing multiple health attributes in coral reef monitoring. For example, autotrophic indices are poor indicators of coral health and condition, but improve when combined with attributes like lipid content and biomass. Copyright © 2017 Elsevier Ltd. All rights reserved.

Working Group 1 "Advanced GNSS Processing Techniques" of the COST Action GNSS4SWEC: Overview of main achievements

NASA Astrophysics Data System (ADS)

Douša, Jan; Dick, Galina; Kačmařík, Michal; Václavovic, Pavel; Pottiaux, Eric; Zus, Florian; Brenot, Hugues; Moeller, Gregor; Hinterberger, Fabian; Pacione, Rosa; Stuerze, Andrea; Eben, Kryštof; Teferle, Norman; Ding, Wenwu; Morel, Laurent; Kaplon, Jan; Hordyniec, Pavel; Rohm, Witold

2017-04-01

The COST Action ES1206 GNSS4SWEC addresses new exploitations of the synergy between developments in GNSS and meteorological communities. The Working Group 1 (Advanced GNSS processing techniques) deals with implementing and assessing new methods for GNSS tropospheric monitoring and precise positioning exploiting all modern GNSS constellations, signals, products etc. Besides other goals, WG1 coordinates development of advanced tropospheric products in support of weather numerical and non-numerical nowcasting. These are ultra-fast and high-resolution tropospheric products available in real time or in a sub-hourly fashion and parameters in support of monitoring an anisotropy of the troposphere, e.g. horizontal gradients and tropospheric slant path delays. This talk gives an overview of WG1 activities and, particularly, achievements in two activities, Benchmark and Real-time demonstration campaigns. For the Benchmark campaign a complex data set of GNSS observations and various meteorological data were collected for a two-month period in 2013 (May-June) which included severe weather events in central Europe. An initial processing of data sets from GNSS and numerical weather models (NWM) provided independently estimated reference parameters - ZTDs and tropospheric horizontal gradients. The comparison of horizontal tropospheric gradients from GNSS and NWM data demonstrated a very good agreement among independent solutions with negligible biases and an accuracy of about 0.5 mm. Visual comparisons of maps of zenith wet delays and tropospheric horizontal gradients showed very promising results for future exploitations of advanced GNSS tropospheric products in meteorological applications such as severe weather event monitoring and weather nowcasting. The Benchmark data set is also used for an extensive validation of line-of-sight tropospheric Slant Total Delays (STD) from GNSS, NWM-raytracing and Water Vapour Radiometer (WVR) solutions. Seven institutions delivered their STDs estimated based on GNSS observations processed using different software and strategies. STDs from NWM ray-tracing came from three institutions using four different NWM models. Results show generally a very good mutual agreement among all solutions from all techniques. The influence of adding not cleaned GNSS post-fit residuals, i.e. residuals that still contains non-tropospheric systematic effects such as multipath, to estimated STDs will be presented. The Real-time demonstration campaign aims at enhancing and assessing ultra-fast GNSS tropospheric products for severe weather and NWM nowcasting. Results are showed from real-time demonstrations as well as offline production simulating real-time using Benchmark campaign.

In Situ Determination of Thermal Profiles during Czochralski Silicon Crystal Growth by an Eddy Current Technique.

NASA Astrophysics Data System (ADS)

Choe, Kwang Su.

An eddy current testing method was developed to continuously monitor crystal growth process and determine thermal profiles in situ during Czochralski silicon crystal growth. The work was motivated by the need to improve the quality of the crystal by controlling thermal gradients and annealing history over the growth cycle. The experimental concept is to monitor intrinsic electrical conductivities of the growing crystal and deduce temperature values from them. The experiments were performed in a resistance-heated Czochralski puller with a 203 mm (8 inch) diameter crucible containing 6.5 kg melt. The silicon crystals being grown were about 80 mm in diameter and monitored by an encircling sensor operating at three different test frequencies (86, 53 and 19 kHz). A one-dimensional analytical solution was employed to translate the detected signals into electrical conductivities. In terms of experiments, the effects of changes in growth condition, which is defined by crystal and crucible rotation rates, crucible position, pull rate, and hot-zone configuration, were investigated. Under a given steady-state condition, the thermal profile was usually stable over the entire length of crystal growth. The profile shifted significantly, however, when the crucible rotation rate was kept too high. As a direct evidence to the effects of melt flow on heat transfer process, a thermal gradient minimum was observed about the crystal/crucible rotation combination of 20/-10 rpm cw. The thermal gradient reduction was still most pronounced when the pull rate or the radiant heat loss to the environment was decreased: a nearly flat axial thermal gradient was achieved when either the pull rate was halved or the height of the exposed crucible wall was effectively doubled. Under these conditions, the average axial thermal gradient along the surface of the crystal was about 4-5 ^{rm o}C/mm. Regardless of growth condition, the three-frequency data revealed radial thermal gradients much larger than what were predicted by existing theoretical models. This discrepancy seems to indicate that optical effects, which are neglected in theoretical modeling, play a major role in the internal heat transfer of the crystal.

A forward model and conjugate gradient inversion technique for low-frequency ultrasonic imaging.

PubMed

van Dongen, Koen W A; Wright, William M D

2006-10-01

Emerging methods of hyperthermia cancer treatment require noninvasive temperature monitoring, and ultrasonic techniques show promise in this regard. Various tomographic algorithms are available that reconstruct sound speed or contrast profiles, which can be related to temperature distribution. The requirement of a high enough frequency for adequate spatial resolution and a low enough frequency for adequate tissue penetration is a difficult compromise. In this study, the feasibility of using low frequency ultrasound for imaging and temperature monitoring was investigated. The transient probing wave field had a bandwidth spanning the frequency range 2.5-320.5 kHz. The results from a forward model which computed the propagation and scattering of low-frequency acoustic pressure and velocity wave fields were used to compare three imaging methods formulated within the Born approximation, representing two main types of reconstruction. The first uses Fourier techniques to reconstruct sound-speed profiles from projection or Radon data based on optical ray theory, seen as an asymptotical limit for comparison. The second uses backpropagation and conjugate gradient inversion methods based on acoustical wave theory. The results show that the accuracy in localization was 2.5 mm or better when using low frequencies and the conjugate gradient inversion scheme, which could be used for temperature monitoring.

Induction of a proton gradient across a gold-supported biomimetic membrane by electroenzymatic H2 oxidation.

PubMed

Gutiérrez-Sanz, Óscar; Tapia, Cristina; Marques, Marta C; Zacarias, Sonia; Vélez, Marisela; Pereira, Inês A C; De Lacey, Antonio L

2015-02-23

Energy-transduction mechanisms in living organisms, such as photosynthesis and respiration, store light and chemical energy in the form of an electrochemical gradient created across a lipid bilayer. Herein we show that the proton concentration at an electrode/phospholipid-bilayer interface can be controlled and monitored electrochemically by immobilizing a membrane-bound hydrogenase. Thus, the energy derived from the electroenzymatic oxidation of H2 can be used to generate a proton gradient across the supported biomimetic membrane. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Surface self-potential patterns related to transmissive fracture trends during a water injection test

NASA Astrophysics Data System (ADS)

DesRoches, A. J.; Butler, K. E.; MacQuarrie, K. TB

2018-03-01

Variations in self-potential (SP) signals were recorded over an electrode array during a constant head injection test in a fractured bedrock aquifer. Water was injected into a 2.2 m interval isolated between two inflatable packers at 44 m depth in a vertical well. Negative SP responses were recorded on surface corresponding to the start of the injection period with strongest magnitudes recorded in electrodes nearest the well. SP response decreased in magnitude at electrodes further from the well. Deflation of the packer system resulted in a strong reversal in the SP signal. Anomalous SP patterns observed at surface at steady state were found to be aligned with dominant fracture strike orientations found within the test interval. Numerical modelling of fluid and current flow within a simplified fracture network showed that azimuthal patterns in SP are mainly controlled by transmissive fracture orientations. The strongest SP gradients occur parallel to hydraulic gradients associated with water flowing out of the transmissive fractures into the tighter matrix and other less permeable cross-cutting fractures. Sensitivity studies indicate that increasing fracture frequency near the well increases the SP magnitude and enhances the SP anomaly parallel to the transmissive set. Decreasing the length of the transmissive fractures leads to more fluid flow into the matrix and into cross-cutting fractures proximal to the well, resulting in a more circular and higher magnitude SP anomaly. Results from the field experiment and modelling provide evidence that surface-based SP monitoring during constant head injection tests has the ability to identify groundwater flow pathways within a fractured bedrock aquifer.

Experiment, monitoring, and gradient methods used to infer climate change effects on plant communities yield consistent patterns

Treesearch

Sarah C. Elmendorf; Gregory H.R. Henry; Robert D. Hollisterd; Anna Maria Fosaa; William A. Gould; Luise Hermanutz; Annika Hofgaard; Ingibjorg I. Jonsdottir; Janet C. Jorgenson; Esther Levesque; Borgbor Magnusson; Ulf Molau; Isla H. Myers-Smith; Steven F. Oberbauer; Christian Rixen; Craig E. Tweedie; Marilyn Walkers

2015-01-01

Inference about future climate change impacts typically relies on one of three approaches: manipulative experiments, historical comparisons (broadly defined to include monitoring the response to ambient climate fluctuations using repeat sampling of plots, dendroecology, and paleoecology techniques), and space-for-time substitutions derived from sampling along...

Effects-based Monitoring with Caged Fathead Minnows: An Exposure Gradient Case Study in the Duluth-Superior Harbor, USA

EPA Science Inventory

Within the Great Lakes there is an increased focus on contaminants of emerging concern (CECs) and consideration of potential effects of chemical mixtures. To further characterize the utility of caged fathead minnows (Pimephales promelas) for effects-based monitoring of CECs, we c...

Effects-based Monitoring with Caged Fathead Minnows: An Exposure Gradient Case Study in the Duluth-Superior Harbor, USA (SETAC meeting)

EPA Science Inventory

Within the Great Lakes there is an increased focus on contaminants of emerging concern (CECs) and consideration of potential effects of chemical mixtures. To further characterize the utility of caged fathead minnows (Pimephales promelas) for effects-based monitoring of CECs, we c...

Assessment of variations in taxonomic diversity, forest structure, and aboveground biomass using remote sensing along an altitudinal gradient in tropical montane forest of Costa Rica

NASA Astrophysics Data System (ADS)

Robinson, C. M.; Saatchi, S. S.; Clark, D.; Fricker, G. A.; Wolf, J.; Gillespie, T. W.; Rovzar, C. M.; Andelman, S.

2012-12-01

This research sought to understand how alpha and beta diversity of plants vary and relate to the three-dimensional vegetation structure and aboveground biomass along environmental gradients in the tropical montane forests of Braulio Carrillo National Park in Costa Rica. There is growing evidence that ecosystem structure plays an important role in defining patterns of species diversity and along with abiotic factors (climate and edaphic) control the phenotypic and functional variations across landscapes. It is well documented that strong subdivisions at local and regional scales are found mainly on geologic or climate gradients. These general determinants of biodiversity are best demonstrated in regions with natural gradients such as tropical montane forests. Altitudinal gradients provide a landscape scale changes through variations in topography, climate, and edaphic conditions on which we tested several theoretical and biological hypotheses regarding drivers of biodiversity. The study was performed by using forest inventory and botanical data from nine 1-ha plots ranging from 100 m to 2800 m above sea level and remote sensing data from airborne lidar and radar sensors to quantify variations in forest structure. In this study we report on the effectiveness of relating patterns of tree taxonomic alpha diversity to three-dimensional structure of a tropical montane forest using lidar and radar observations of forest structure and biomass. We assessed alpha and beta diversity at the species, genus, and family levels utilizing datasets provided by the Terrestrial Ecology Assessment and Monitoring (TEAM) Network. Through the comparison to active remote sensing imagery, our results show that there is a strong relationship between forest 3D-structure, and alpha and beta diversity controlled by variations in abiotic factors along the altitudinal gradient. Using spatial analysis with the aid of remote sensing data, we find distinct patterns along the environmental gradients defining species turnover and changes in functional diversity. The study will provide novel approaches to use detailed spatial information from remote sensing data to study relations between functional and taxonomic dimensions of diversity.

Transport of fecal-derived microorganisms from latrine ponds to aquifers in Bangladesh

NASA Astrophysics Data System (ADS)

Knappett, P. S.; McKay, L. D.; Layton, A.; Alam, M.; Williams, D.; Huq, M. R.; Mailloux, B. J.; Ferguson, A.; Feighery, J. E.; Culligan, P. J.; Escamilla, V.; Emch, M.; Akita, Y.; Serre, M. L.; Perfect, E.; Gentry, R. W.; Ahmed, K. M.; van Geen, A.

2009-12-01

Groundwater has been the principal source of drinking water for over 100 million people in rural Bangladesh for the past twenty years. The shallow depths and simple construction of the private wells has raised concern that these wells may be receiving fecal contamination from the densely populated rural areas with poor sanitation, contributing to high rates of diarrheal disease. Ponds are ubiquitous in Bangladesh, serving multiple purposes, including receiving fecal effluent from latrines, and private wells are frequently located in close proximity to these potential groundwater contamination sources. After detecting E. coli in up to 70% of private and monitoring wells throughout a village in Araihazar, the numerous ponds throughout the village were hypothesized to be sources of this contamination. To test this hypothesis 9 lateral transects of 4 monitoring wells each, 7 m deep and placed 1 m apart, were installed radiating away from four ponds of contrasting ages and near surface geology. These transects were monitored throughout the year to look for evidence that the ponds were contributing E. coli to the groundwater system. During the dry season from September 2008 to May 2009 no E. coli was observed in the shallow monitoring wells. In contrast, when the rains began in June 2009 several of the transects showed increasing water levels and E. coli with proximity to the pond, providing evidence that some ponds were acting as a contamination point source. A major rainfall event was simulated in June 2009 in each of the four ponds, raising the water level by 20 to 30 cm while adjacent transects were monitored. In two recently dug, deep ponds E. coli travelled up to 6 m into the medium sand aquifer within 24 hours as a result of the simulated rainfall event. In the two older ponds, which had well developed silt layers on the bottom or were emplaced in silty aquifers little E. coli was detected in the adjacent monitoring wells under natural or forced gradient conditions. For the two ponds with sandy bottoms, the pulse of E. coli was attenuated exponentially with distance, but elevated levels of E. coli remained in the aquifer for more than 10 days after the initial flooding of the ponds. Thus, during the wet season, proximity to young, deep ponds cut into sandy aquifers is a risk factor for diarrheal disease in the adjacent households. The ponds alone, however, cannot explain the spatial and temporal distribution of E. coli throughout the village aquifer, and additional sources require investigation.

Quantitative determination of major active components in Ginkgo biloba dietary supplements by liquid chromatography/mass spectrometry.

PubMed

Ding, Shujing; Dudley, Ed; Plummer, Sue; Tang, Jiandong; Newton, Russell P; Brenton, A Gareth

2006-01-01

A reversed-phase high-performance liquid chromatography/electrospray ionisation mass spectrometry (RP-HPLC/ESI-MS) method was developed and validated for the simultaneous determination of ten major active components in Ginkgo biloba extract (bilobalide, ginkgolides A, B, C, quercetin, kaempferol, isorhamnetin, rutin hydrate, quercetin-3-beta-D-glucoside and quercitrin hydrate) which have not been previously reported to be quantified in a single analysis. The ten components exhibit baseline separation in 50 min by C18 chromatography using a water/1:1 (v/v) methanol/acetonitrile gradient. Quantitation was performed using negative ESI-MS in selected ion monitoring (SIM) mode. Good reproducibility and recovery were obtained by this method. The sensitivity of both UV and different mass spectrometry modes (full scan, selected ion monitoring (SIM), and selected reaction monitoring (SRM)) were compared and both quantitation with and without internal standard were evaluated. The analysis of Ginkgo biloba commercial products showed remarkable variations in the rutin and quercetin content as well as the terpene lactone contents although all the products satisfy the conventional quality control method. Copyright 2006 John Wiley & Sons, Ltd.

Can Nocturnal Cold Air Drainage be Used to Monitor Ecosystem Function?

NASA Astrophysics Data System (ADS)

Pypker, T. G.; Unsworth, M. H.; Sulzman, E. W.; Lamb, B.; Allwine, G.; Mix, A. C.; Bond, B. J.

2005-12-01

Ecosystem carbon dynamics in flat, uniform terrain are commonly studied using standard micrometeorological techniques such as eddy covariance or gradient methods. But many of the world's ecosystems are in complex topography that is inappropriate for these methods. Nocturnal cold air drainage commonly occurs in mountainous terrain. This drainage provides an opportunity to monitor ecosystem carbon dynamics because as air flows downhill through a watershed, it collects respired CO2 from the soil and vegetation. If the nocturnal drainage can be treated as a river of air flowing down a valley, sampling this air from a tower at the base of a watershed could provide an estimate of ecosystem respiration and the 12C/13C ratio. To interpret the measured CO2 and the 12C/13C ratio, the characteristics of the drainage and the footprint (source area) of air passing the tower must be understood. To explore the potential of using nocturnal cold air drainage we built a 37 m tower at the base of a deeply incised watershed of ~40 y-old Douglas-fir in the Oregon Cascades. At various heights on the tower we monitored air temperature, wind speed/direction, and the CO2 concentration and 12C/13C isotopic ratio with a combination of thermistors, sonic anemometers (2-D and 3-D) and a CO2 profile system. The temperature gradient along the axis of the watershed was monitored by 30 temperature sensors from the base to the top of the watershed. The maximum drainage windspeeds on the tower occurred near sunset and, unlike past reports of cold air drainage, this drainage was very deep (> 37 m). The drainage became well mixed when the vertical profile of potential temperature became isothermal. It remained well mixed through the night into the early morning. The drainage occurred on most summer nights and typically provided a range of CO2 (> 60 ppm) sufficient for "Keeling plot" analysis. In September 2005, we released a tracer in the watershed (SF6) to determine the varying footprint size of the tower. The footprint size and windspeed varied throughout the night, resulting in a change in the CO2 concentration at the tower. Further analysis will determine how the CO2 passing the tower is altered by entrainment of the air overlying the drainage and the change in the source area of the drainage.

Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species

PubMed Central

Settanni, Luca; Valmorri, Sara; van Sinderen, Douwe; Suzzi, Giovanna; Paparella, Antonello; Corsetti, Aldo

2006-01-01

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs. PMID:16672538

Modeling energy flow and nutrient cycling in natural semiarid grassland ecosystems with the aid of thematic mapper data

NASA Technical Reports Server (NTRS)

Lewis, James K.

1987-01-01

Energy flow and nutrient cycling were modeled as affected by herbivory on selected intensive sites along gradients of precipitation and soils, validating the model output by monitoring selected parameters with data derived from the Thematic Mapper (TM). Herbivore production was modeled along the gradient of soils and herbivory, and validated with data derived from TM in a spatial data base.

Stability-indicating UPLC method for determination of Valsartan and their degradation products in active pharmaceutical ingredient and pharmaceutical dosage forms.

PubMed

Krishnaiah, Ch; Reddy, A Raghupathi; Kumar, Ramesh; Mukkanti, K

2010-11-02

A simple, precise, accurate stability-indicating gradient reverse phase ultra-performance liquid chromatographic (RP-UPLC) method was developed for the quantitative determination of purity of Valsartan drug substance and drug products in bulk samples and pharmaceutical dosage forms in the presence of its impurities and degradation products. The method was developed using Waters Aquity BEH C18 (100 mm x 2.1 mm, 1.7 microm) column with mobile phase containing a gradient mixture of solvents A and B. The eluted compounds were monitored at 225 nm, the run time was within 9.5 min, which Valsartan and its seven impurities were well separated. Valsartan was subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation. Valsartan was found to degrade significantly in acid and oxidative stress conditions and stable in base, hydrolytic and photolytic degradation conditions. The degradation products were well resolved from main peak and its impurities, proving the stability-indicating power of the method. The developed method was validated as per international conference on harmonization (ICH) guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness. This method was also suitable for the assay determination of Valsartan in pharmaceutical dosage forms.

Rainfall characteristics and thresholds for periglacial debris flows in the Parlung Zangbo Basin, southeast Tibetan Plateau

NASA Astrophysics Data System (ADS)

Deng, Mingfeng; Chen, Ningsheng; Ding, Haitao

2018-02-01

The Parlung Zangbo Basin in the southeastern Tibet Plateau is affected by the summer monsoon from the Indian Ocean, which produces large rainfall gradients in the basin. Rainfall data during 2012-2015 from five new meteorological stations are used to analyse the rainfall characteristics. The daily rainfall, rainfall duration, mean rainfall intensity, and peak rainfall intensity are consistent, but sometimes contrasting. For example, these values decrease with increasing altitude, and the gradient is large downstream and small upstream, respectively. Moreover, the rainfall intensity peaks between 01:00 and 06:00 and increases during the afternoon. Based on the analysis of 14 debris flow cases in the basin, differences in the rainfall threshold differ depending on the location as sediment varieties. The sediment in the middle portions of the basin is wet and well structured; thus, long-duration, high-intensity rainfall is required to generate debris flows. Ravels in the upstream area are arid and not well structured, and short-duration rainfall is required to trigger debris flows. Between the above two locations, either long-duration, low-intensity rainfall or short-duration, high-intensity rainfall could provoke debris flows. Clearly, differences in rainfall characteristics and rainfall thresholds that are associated with the location must be considered in debris flow monitoring and warnings.

Systematic generation of buffer systems for pH gradient ion exchange chromatography and their application.

PubMed

Kröner, Frieder; Hubbuch, Jürgen

2013-04-12

pH gradient protein separations are widely used techniques in the field of protein analytics, of which isoelectric focusing is the most well known application. The chromatographic variant, based on the formation of pH gradients in ion exchange columns is only rarely applied due to the difficulties to form controllable, linear pH gradients over a broad pH range. This work describes a method for the systematic generation of buffer compositions with linear titration curves, resulting in well controllable pH gradients. To generate buffer compositions with linear titration curves an in silico method was successfully developed. With this tool, buffer compositions for pH gradient ion exchange chromatography with pH ranges spanning up to 7.5 pH units were established and successfully validated. Subsequently, the buffer systems were used to characterize the elution behavior of 22 different model proteins in cation and anion exchange pH gradient chromatography. The results of both chromatographic modes as well as isoelectric focusing were compared to describe differences in between the methods. Copyright © 2013 Elsevier B.V. All rights reserved.

Monitoring trace metals in seawater using a diffusive gradient in thin film probe in Ulsan Bay, East Sea, Korea: Comparison with transplanted mussels

NASA Astrophysics Data System (ADS)

Kim, Mi Seon; Choi, Man Sik; Kim, Chan-Kook

2016-03-01

To evaluate the applicability of a diffusive gradient in thin film (DGT) probe for monitoring dissolved metals in coastal seawater, DGT-labile metal concentrations were compared with total dissolved metal concentrations using spiked and natural seawater samples in the laboratory and transplanted mussels ( Mytilus galloprovincialis). This was achieved through the simultaneous deployment of DGT probes and transplanted mussels in Ulsan Bay during winter and summer. DGT-labile metal concentrations were 45% (Cu) ~ 90% (Zn) of total dissolved concentrations, and the order of non-labile concentrations was Cu > Pb > Co ~ Ni > Cd ~ Zn in both metal-contaminated and non-contaminated seawater samples, which was similar to the order of stability of metal complexes in the Irving-Williams series. The overall variability of the DGT probe results within and between tanks was less than 10% (relative standard deviation: RSD) for all the metals tested during a 48-h deployment. The accumulation of metals, as determined by DGT probes, represented the spatial gradients better than the transplanted mussels did for all of the metals tested, and the extent of metal accumulation in mussels differed depending on the metal. The comparison of results for the DGT probe and the transplanted mussels in two seasons (winter and summer) suggested that metal accumulation in mussels was controlled by the physiological factors of mussels and partly by their diet (particulate metal loadings). The DGT probe could be used as a monitoring tool for dissolved metals in coastal seawater because its results explained only labile species. When using the DGT probe, slightly more than half of the total dissolved concentration in seawater samples for all the metals investigated displayed timeintegrated properties and distinct spatial gradients from pristine to metal-contaminated seawater.

High resolution temperature mapping of gas turbine combustor simulator exhaust with femtosecond laser induced fiber Bragg gratings

NASA Astrophysics Data System (ADS)

Walker, Robert B.; Yun, Sangsig; Ding, Huimin; Charbonneau, Michel; Coulas, David; Lu, Ping; Mihailov, Stephen J.; Ramachandran, Nanthan

2017-04-01

Femtosecond infrared (fs-IR) laser written fiber Bragg gratings (FBGs), have demonstrated great potential for extreme sensing. Such conditions are inherent in advanced gas turbine engines under development to reduce greenhouse gas emissions; and the ability to measure temperature gradients in these harsh environments is currently limited by the lack of sensors and controls capable of withstanding the high temperature, pressure and corrosive conditions present. This paper discusses fabrication and deployment of several fs-IR written FBG arrays, for monitoring exhaust temperature gradients of a gas turbine combustor simulator. Results include: contour plots of measured temperature gradients, contrast with thermocouple data.

Modeling of salt and pH gradient elution in ion-exchange chromatography.

PubMed

Schmidt, Michael; Hafner, Mathias; Frech, Christian

2014-01-01

The separation of proteins by internally and externally generated pH gradients in chromatofocusing on ion-exchange columns is a well-established analytical method with a large number of applications. In this work, a stoichiometric displacement model was used to describe the retention behavior of lysozyme on SP Sepharose FF and a monoclonal antibody on Fractogel SO3 (S) in linear salt and pH gradient elution. The pH dependence of the binding charge B in the linear gradient elution model is introduced using a protein net charge model, while the pH dependence of the equilibrium constant is based on a thermodynamic approach. The model parameter and pH dependences are calculated from linear salt gradient elutions at different pH values as well as from linear pH gradient elutions at different fixed salt concentrations. The application of the model for the well-characterized protein lysozyme resulted in almost identical model parameters based on either linear salt or pH gradient elution data. For the antibody, only the approach based on linear pH gradients is feasible because of the limited pH range useful for salt gradient elution. The application of the model for the separation of an acid variant of the antibody from the major monomeric form is discussed. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Refined gradient theory of scale-dependent superthin rods

NASA Astrophysics Data System (ADS)

Lurie, S. A.; Kuznetsova, E. L.; Rabinskii, L. N.; Popova, E. I.

2015-03-01

A version of the refined nonclassical theory of thin beams whose thickness is comparable with the scale characteristic of the material structure is constructed on the basis of the gradient theory of elasticity which, in contrast to the classical theory, contains some additional physical characteristics depending on the structure scale parameters and is therefore most appropriate for modeling the strains of scale-dependent systems. The fundamental conditions for the well-posedness of the gradient theories are obtained for the first time, and it is shown that some of the known applied gradient theories do not generally satisfy the well-posedness criterion. A version of the well-posed gradient strain theory which satisfies the symmetry condition is proposed. The well-posed gradient theory is then used to implement the method of kinematic hypotheses for constructing a refined theory of scale-dependent beams. The equilibrium equations of the refined theory of scale-dependent Timoshenko and Bernoulli beams are obtained. It is shown that the scale effects are localized near the beam ends, and therefore, taking the scale effects into account does not give any correction to the bending rigidity of long beams as noted in the previously published papers dealing with the scale-dependent beams.

Gradient and shim technologies for ultra high field MRI

PubMed Central

Winkler, Simone A.; Schmitt, Franz; Landes, Hermann; DeBever, Josh; Wade, Trevor; Alejski, Andrew

2017-01-01

Ultra High Field (UHF) MRI requires improved gradient and shim performance to fully realize the promised gains (SNR as well as spatial, spectral, diffusion resolution) that higher main magnetic fields offer. Both the more challenging UHF environment by itself, as well as the higher currents used in high performance coils, require a deeper understanding combined with sophisticated engineering modeling and construction, to optimize gradient and shim hardware for safe operation and for highest image quality. This review summarizes the basics of gradient and shim technologies, and outlines a number of UHF-related challenges and solutions. In particular, Lorentz forces, vibroacoustics, eddy currents, and peripheral nerve stimulation are discussed. Several promising UHF-relevant gradient concepts are described, including insertable gradient coils aimed at higher performance neuroimaging. PMID:27915120

Acoustic Emission Behavior of Early Age Concrete Monitored by Embedded Sensors.

PubMed

Qin, Lei; Ren, Hong-Wei; Dong, Bi-Qin; Xing, Feng

2014-10-02

Acoustic emission (AE) is capable of monitoring the cracking activities inside materials. In this study, embedded sensors were employed to monitor the AE behavior of early age concrete. Type 1-3 cement-based piezoelectric composites, which had lower mechanical quality factor and acoustic impedance, were fabricated and used to make sensors. Sensors made of the composites illustrated broadband frequency response. In a laboratory, the cracking of early age concrete was monitored to recognize different hydration stages. The sensors were also embedded in a mass concrete foundation to localize the temperature gradient cracks.

Toward development of mobile application for hand arthritis screening.

PubMed

Akhbardeh, Farhad; Vasefi, Fartash; Tavakolian, Kouhyar; Bradley, David; Fazel-Rezai, Reza

2015-01-01

Arthritis is one of the most common health problems affecting people throughout the world. The goal of the work presented in this paper is to provide individuals, who may be developing or have developed arthritis, with a mobile application to assess and monitor the progress of their disease using their smartphone. The image processing algorithm includes finger border detection algorithm to monitor joint thickness and angular deviation abnormalities, which are common symptoms of arthritis. In this work, we have analyzed and compared gradient, thresholding and Canny algorithms for border detection. The effect of image spatial resolution (down-sampling) is also investigated. The results calculated based on 36 joint measurements show that the mean errors for gradient, thresholding, and Canny methods are 0.20, 2.13, and 2.03 mm, respectively. In addition, the average error for different image resolutions is analyzed and the minimum required resolution is determined for each method. The results confirm that recent smartphone imaging capabilities can provide enough accuracy for hand border detection and finger joint analysis based on gradient method.

Concurrent recording of RF pulses and gradient fields - comprehensive field monitoring for MRI.

PubMed

Brunner, David O; Dietrich, Benjamin E; Çavuşoğlu, Mustafa; Wilm, Bertram J; Schmid, Thomas; Gross, Simon; Barmet, Christoph; Pruessmann, Klaas P

2016-09-01

Reconstruction of MRI data is based on exact knowledge of all magnetic field dynamics, since the interplay of RF and gradient pulses generates the signal, defines the contrast and forms the basis of resolution in spatial and spectral dimensions. Deviations caused by various sources, such as system imperfections, delays, eddy currents, drifts or externally induced fields, can therefore critically limit the accuracy of MRI examinations. This is true especially at ultra-high fields, because many error terms scale with the main field strength, and higher available SNR renders even smaller errors relevant. Higher baseline field also often requires higher acquisition bandwidths and faster signal encoding, increasing hardware demands and the severity of many types of hardware imperfection. To address field imperfections comprehensively, in this work we propose to expand the concept of magnetic field monitoring to also encompass the recording of RF fields. In this way, all dynamic magnetic fields relevant for spin evolution are covered, including low- to audio-frequency magnetic fields as produced by main magnets, gradients and shim systems, as well as RF pulses generated with single- and multiple-channel transmission systems. The proposed approach permits field measurements concurrently with actual MRI procedures on a strict common time base. The combined measurement is achieved with an array of miniaturized field probes that measure low- to audio-frequency fields via (19) F NMR and simultaneously pick up RF pulses in the MRI system's (1) H transmit band. Field recordings can form the basis of system calibration, retrospective correction of imaging data or closed-loop feedback correction, all of which hold potential to render MRI more robust and relax hardware requirements. The proposed approach is demonstrated for a range of imaging methods performed on a 7 T human MRI system, including accelerated multiple-channel RF pulses. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

Evaluating Area-Based Socioeconomic Status Indicators for Monitoring Disparities within Health Care Systems: Results from a Primary Care Network

PubMed Central

Berkowitz, Seth A; Traore, Carine Y; Singer, Daniel E; Atlas, Steven J

2015-01-01

Objective To determine which area-based socioeconomic status (SES) indicator is best suited to monitor health care disparities from a delivery system perspective. Data Sources/Study Setting 142,659 adults seen in a primary care network from January 1, 2009 to December 31, 2011. Study Design Cross-sectional, comparing associations between area-based SES indicators and patient outcomes. Data Collection Address data were geocoded to construct area-based SES indicators at block group (BG), census tract (CT), and ZIP code (ZIP) levels. Data on health outcomes were abstracted from electronic records. Relative indices of inequality (RIIs) were calculated to quantify disparities detected by area-based SES indicators and compared to RIIs from self-reported educational attainment. Principal Findings ZIP indicators had less missing data than BG or CT indicators (p < .0001). Area-based SES indicators were strongly associated with self-report educational attainment (p < .0001). ZIP, BG, and CT indicators all detected expected SES gradients in health outcomes similarly. Single-item, cut point defined indicators performed as well as multidimensional indices and quantile indicators. Conclusions Area-based SES indicators detected health outcome differences well and may be useful for monitoring disparities within health care systems. Our preferred indicator was ZIP-level median household income or percent poverty, using cut points. PMID:25219917

First evaluation of foraminiferal metabarcoding for monitoring environmental impact from an offshore oil drilling site.

PubMed

Laroche, Olivier; Wood, Susanna A; Tremblay, Louis A; Ellis, Joanne I; Lejzerowicz, Franck; Pawlowski, Jan; Lear, Gavin; Atalah, Javier; Pochon, Xavier

2016-09-01

At present, environmental impacts from offshore oil and gas activities are partly determined by measuring changes in macrofauna diversity. Morphological identification of macrofauna is time-consuming, expensive and dependent on taxonomic expertise. In this study, we evaluated the applicability of using foraminiferal-specific metabarcoding for routine monitoring. Sediment samples were collected along distance gradients from two oil platforms off Taranaki (New Zealand) and their physico-chemical properties, foraminiferal environmental DNA/RNA, and macrofaunal composition analyzed. Macrofaunal and foraminiferal assemblages showed similar shifts along impact gradients, but responded differently to environmental perturbations. Macrofauna were affected by hypoxia, whereas sediment grain size appeared to drive shifts in foraminifera. We identified eight foraminiferal molecular operational taxonomic units that have potential to be used as bioindicator taxa. Our results show that metabarcoding represents an effective tool for assessing foraminiferal communities near offshore oil and gas platforms, and that it can be used to complement current monitoring techniques. Copyright © 2016 Elsevier Ltd. All rights reserved.

Holocene precipitation in the coastal temperate rainforest complex of southern British Columbia, Canada

NASA Astrophysics Data System (ADS)

Brown, K. J.; Fitton, R. J.; Schoups, G.; Allen, G. B.; Wahl, K. A.; Hebda, R. J.

2006-11-01

Pollen data from 69 surface samples from Vancouver Island, Canada, were used to develop a ratio index of precipitation, Douglas fir-western hemlock index (DWHI). DWHI ratios were combined with interpolated estimates of mean annual precipitation to develop pollen-based precipitation transfer functions. The optimal regression model, with a predictive range of 960-2600 mm, was applied to 10 Holocene lake sediment records distributed across a ˜150 km long coastal-inland precipitation gradient. Predicted precipitation was spatially modelled in a geographic information system to examine the spatio-temporal history of precipitation from this representative portion of the coastal temperate rainforest (CTR) complex of western North America. The reconstructions show widespread early Holocene dry conditions coupled with a steep east-west precipitation gradient. Thereafter, the modern precipitation gradient established 7000 years ago, illustrating that the CTR complex has experienced marked short-distance east-west changes in precipitation in the past. Changes in the abundance of arboreal and non-arboreal vegetation, as well as fire disturbance, are often concomitant with changes in Holocene precipitation. Given the precipitation and vegetation history of the region, conservation initiatives should focus on the moist outer coastal zone since it appears to have the greatest amount of resilience to perturbations in precipitation, whereas monitoring programs for signs of climate change should be initiated in central and eastern areas as they appear sensitive to changes in the moisture regime.

A scrutiny of heterogeneity at the TCE Source Area BioREmediation (SABRE) test site

NASA Astrophysics Data System (ADS)

Rivett, M.; Wealthall, G. P.; Mcmillan, L. A.; Zeeb, P.

2015-12-01

A scrutiny of heterogeneity at the UK's Source Area BioREmediation (SABRE) test site is presented to better understand how spatial heterogeneity in subsurface properties and process occurrence may constrain performance of enhanced in-situ bioremediation (EISB). The industrial site contained a 25 to 45 year old trichloroethene (TCE) dense non-aqueous phase liquid (DNAPL) that was exceptionally well monitored via a network of multilevel samplers and high resolution core sampling. Moreover, monitoring was conducted within a 3-sided sheet-pile cell that allowed a controlled streamtube of flow to be drawn through the source zone by an extraction well. We primarily focus on the longitudinal transect of monitoring along the length of the cell that provides a 200 groundwater point sample slice along the streamtube of flow through the DNAPL source zone. TCE dechlorination is shown to be significant throughout the cell domain, but spatially heterogeneous in occurrence and progress of dechlorination to lesser chlorinated ethenes - it is this heterogeneity in dechlorination that we primarily scrutinise. We illustrate the diagnostic use of the relative occurrence of TCE parent and daughter compounds to confirm: dechlorination in close proximity to DNAPL and enhanced during the bioremediation; persistent layers of DNAPL into which gradients of dechlorination products are evident; fast flowpaths through the source zone where dechlorination is less evident; and, the importance of underpinning flow regime understanding on EISB performance. Still, even with such spatial detail, there remains uncertainty over the dataset interpretation. These includes poor closure of mass balance along the cell length for the multilevel sampler based monitoring and points to needs to still understand lateral flows (even in the constrained cell), even greater spatial resolution of point monitoring and potentially, not easily proven, ethene degradation loss.

Intra-urban and street scale variability of BTEX, NO 2 and O 3 in Birmingham, UK: Implications for exposure assessment

NASA Astrophysics Data System (ADS)

Vardoulakis, Sotiris; Solazzo, Efisio; Lumbreras, Julio

2011-09-01

Automatic monitoring networks have the ability of capturing air pollution episodes, as well as short- and long-term air quality trends in urban areas that can be used in epidemiological studies. However, due to practical constraints (e.g. cost and bulk of equipment), the use of automatic analysers is restricted to a limited number of roadside and background locations within a city. As a result, certain localised air pollution hotspots may be overlooked or overemphasised, especially near heavily trafficked street canyons and intersections. This has implications for compliance with regulatory standards and may cause exposure misclassification in epidemiological studies. Apart from automatic analysers, low cost passive diffusion tubes can be used to characterise the spatial variability of air pollution in urban areas. In this study, BTEX, NO 2 and O 3 data from a one-year passive sampling survey were used to characterise the intra-urban and street scale spatial variability of traffic-related pollutants in Birmingham (UK). In addition, continuous monitoring of NO 2, NO x, O 3, CO, SO 2, PM 10 and PM 2.5 from three permanent monitoring sites was used to identify seasonal and annual pollution patterns. The passive sampling measurements allowed us to evaluate the representativeness of a permanent roadside monitoring site that has recorded some of the highest NO 2 and PM 10 concentrations in Birmingham in recent years. Dispersion modelling was also used to gain further insight into pollutant sources and dispersion characteristics at this location. The strong spatial concentration gradients observed in busy streets, as well as the differences between roadside and urban background levels highlight the importance of appropriate positioning of air quality monitoring equipment in cities.

Final report : groundwater monitoring at Centralia, Kansas, inSeptember-October 2005 and March 2006, with expansion of the monitoringnetwork in January 2006.

DOE Office of Scientific and Technical Information (OSTI.GOV)

LaFreniere, L. M.

2006-12-07

This document reports the results of groundwater sampling in September-October 2005 and March 2006 at the grain storage facility formerly operated at Centralia, Kansas, by the Commodity Credit Corporation of the U.S. Department of Agriculture (CCC/USDA). These activities were the first and second twice yearly sampling events of the two-year monitoring program approved by the CCC/USDA and Kansas Department of Health and Environment (KDHE) project managers. The initial monitoring network sampled in September and October 2005 consisted of six monitoring wells (MW1-MW6) installed in 2004, plus five groundwater piezometers (SB01, SB04, SB05, SB08, SB09) installed in 2002. The combined September-Octobermore » 2005 sampling was the first monitoring event in the planned two-year program for Centralia. The groundwater samples collected in both September and October were analyzed for volatile organic compounds (VOCs), and samples collected in September were analyzed for dissolved hydrogen and additional groundwater parameters to aid in evaluating the potential for reductive dechlorination processes. After the monitoring in September-October 2005, Argonne recommended expansion of the initial monitoring network. Previous sampling (August 2004) had already suggested that this network of six monitoring wells and five piezometers was inadequate to delineate the extent of the carbon tetrachloride plume. With the approval of the CCC/USDA and KDHE project managers, the monitoring network was expanded in January 2006 through the installation of four additional monitoring wells (MW7-MW10) and one new piezometer (SB07R) to replace a damaged piezometer (the former SB07). Details of the monitoring well and piezometer installations are reported in this document. The expanded monitoring network of ten monitoring wells (MW01-MW10) and six piezometers (SB01, SB04, SB05, SB07R, SB08, and SB09) was sampled in March 2006. This March 2006 sampling was the second monitoring event in the planned two-year program. Results of analyses for VOCs showed further increases in contaminant levels and expansion of the carbon tetrachloride plume toward the south and west from the former CCC/USDA facility. The groundwater samples collected in March 2006 were also analyzed for additional groundwater parameters to aid in the evaluation of the potential for reductive dechlorination processes. Preliminary screening of groundwater parameters provided limited evidence that reductive dechlorination of carbon tetrachloride is taking place at some locations on the former CCC/USDA facility. Groundwater levels measured manually in September 2005, March 2006, and June 2006 were used to map the potentiometric surface at Centralia. Overall, these results were consistent with each other and with previous measurements, generally indicating a groundwater flow direction toward the south-southwest from the former CCC/USDA facility. Data recorders installed in wells MW01-MW06 in August 2004 are gathering long-term data on the groundwater elevation and gradient. Data downloaded in March 2005, September 2005, and June 2006 indicate that two wells north and west of the former CCC/USDA facility boundary show distinct, transient and seasonal water level variations. In contrast, two different wells southwest and south of the former facility boundary show virtually no response to the same events. The first two monitoring events of the planned two-year monitoring program for Centralia have demonstrated increased carbon tetrachloride concentrations and lateral expansion of the contaminated zone. Argonne recommends that the CCC/USDA and KDHE project managers consider development and approval of a work plan to expedite the selection and implementation of an active remedial alternative addressing the concentrated areas of groundwater contamination before the end of the two-year monitoring program in 2007.« less

SOFI/Substrate integrity testing for cryogenic propellant tanks at extreme thermal gradient conditions

NASA Astrophysics Data System (ADS)

Haynes, M.; Fabian, P.

2015-12-01

Liquid propellant tank insulation for space flight requires low weight as well as high insulation factors. Use of Spray-On Foam Insulation (SOFI) is an accepted, cost effective technique for insulating a single wall cryogenic propellant tank and has been used extensively throughout the aerospace industry. Determining the bond integrity of the SOFI to the metallic substrate as well as its ability to withstand the in-service strains, both mechanical and thermal, is critical to the longevity of the insulation. This determination has previously been performed using highly volatile, explosive cryogens, which increases the test costs enormously, as well as greatly increasing the risk to both equipment and personnel. CTD has developed a new test system, based on a previous NASA test that simulates the mechanical and thermal strains associated with filling a large fuel tank with a cryogen. The test enables a relatively small SOFI/substrate sample to be monitored for any deformations, delaminations, or disjunctures during the cooling and mechanical straining process of the substrate, and enables the concurrent application of thermal and physical strains to two specimens at the same time. The thermal strains are applied by cooling the substrate to the desired cryogen temperature (from 4 K to 250 K) while maintaining the outside surface of the SOFI foam at ambient conditions. Multiple temperature monitoring points are exercised to ensure even cooling across the substrate, while at the same time, surface temperatures of the SOFI can be monitored to determine the heat flow. The system also allows for direct measurement of the strains in the substrate during the test. The test system as well as test data from testing at 20 K, for liquid Hydrogen simulation, will be discussed.

Evaluation of potential gas clogging associated with managed aquifer recharge from a spreading basin, southwestern Utah, U.S.A.

USGS Publications Warehouse

Heilweil, Victor M.; Marston, Thomas

2013-01-01

Sand Hollow Reservoir in southwestern Utah, USA, is operated for both surface-water storage and managed aquifer recharge via infiltration from surface basin spreading to the underlying Navajo Sandstone. The total volume of estimated recharge from 2002 through 2011 was 131 Mm3., resulting in groundwater levels rising as much as 40 m. Hydraulic and hydrochemical data from the reservoir and various monitoring wells in Sand Hollow were used to evaluate the timing and location or reservoir recharge moving through the aquifer, along either potential clogging from trapped gases in pore throats, siltation, or algal mats. Several hyrdochemical tracers indicated this recharge had arrived at four monitoring wells located within about 300 m of the reservoir by 2012. At these wells, peak total dissolved-gas pressures exceeded two atmospheres (>1,500 mm mercury) and dissolved oxygen approached three times atmospherically equilibrated concentrations (>25 mg/L). these field parameters indicate that large amounts of gas trapped in pore spaces beneath the water table have dissolved. Lesser but notable increases in these dissolved-gas parameters (without increases in other indicators such as chloride-to-bromide ratios) at monitoring wells farther away (>300 m) indicate moderate amounts of in-situ sir entrapment and dissolution caused by the rise in regional groundwater levels. This is confirmed by hydrochemical difference between these sites and wells closer to the reservoir where recharge had already arrived. As the reservoir was being filled by 2002, managed aquifer recharge rates were initially very high (1.5 x 10-4 cm/s) with the vadose zone becoming saturated beneath and surrounding the reservoir. These rates declined to less than 3.5 x 10-6 cm/s during 2008. The 2002-08 decrease was likely associated with a declining regional hydraulic gradient and clogging. Increasing recharge rates during mid-2009 through 2010 may have been partly caused by dissolution of air bubbles initially entrapped in the aquifer matrix. Theoretical gas dissolution rates, coupled with field evidence of a decline iin total dissolved-gas pressure and dissolved oxygen from nearby monitoring wells, support the timing of this gas dissipation.

Surface water-ground water interactions along the lower Dungeness River and vertical hydraulic conductivity of streambed sediments, Clallam County, Washington, September 1999-July 2001

USGS Publications Warehouse

Simonds, F. William; Sinclair, Kirk A.

2002-01-01

The Dungeness River emerges from the Olympic Mountains and flows generally north toward the Strait of Juan De Fuca, crossing the broad, fertile alluvial fan of the Sequim-Dungeness peninsula in northeastern Clallam County, Washington. Increasing competition for the peninsula's ground-water resources, changing water-use patterns, and recent requirements to maintain minimum in-stream flows to enhance endangered salmon and trout populations have severely strained the peninsula's water resources and necessitated a better understanding of the interaction between surface water and groundwater. Three methods were used to characterize the interchange between surface water and groundwater along the lower 11.8 miles of the Dungeness River corridor between September 1999 and July 2001. In-stream mini-piezometers were used to measure vertical hydraulic gradients between the river and the water-table aquifer at 27 points along the river and helped to define the distribution of gaining and losing stream reaches. Seepage runs were used to quantify the net volume of water exchanged between the river and ground water within each of five river reaches, termed 'seepage reaches.' Continuous water-level and water-temperature monitoring at two off-stream well transects provided data on near-river horizontal hydraulic gradients and temporal patterns of water exchange for a representative gaining stream reach and a representative losing stream reach. Vertical hydraulic gradients in the mini-piezometers generally were negative between river miles 11.8 and 3.6, indicating loss of water from the river to ground water. Gradients decreased in the downstream direction from an average of -0.86 at river mile 10.3 to -0.23 at river mile 3.7. Small positive gradients (+0.01 to +0.02) indicating ground-water discharge occurred in three localized reaches below river mile 3.7. Data from the seepage runs and off-stream transect wells supported and were generally consistent with the mini-piezometer findings. An exception occurred between river miles 8.1 and 5.5 where seepage results showed a small gain and the mini-piezometers showed negative gradients. Vertical hydraulic conductivity of riverbed sediments was estimated using hydraulic gradients measured with the mini-piezometers and estimated seepage fluxes. The resulting conductivity values ranged from an average of 1 to 29 feet per day and are similar to values reported for similar river environments elsewhere. The results of this study will be used to calibrate a transient, three-dimensional ground-water flow model of the Sequim-Dungeness peninsula. The model will be used to assess the potential effects on ground-water levels and river flows that result from future water use and land-use changes on the peninsula.

Nonlinear verification of a linear critical gradient model for energetic particle transport by Alfven eigenmodes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bass, Eric M.; Waltz, R. E.

Here, a “stiff transport” critical gradient model of energetic particle (EP) transport by EPdriven Alfven eigenmodes (AEs) is verified against local nonlinear gyrokinetic simulations of a well-studied beam-heated DIII-D discharge 146102. A greatly simplifying linear “recipe” for the limiting EP-density gradient (critical gradient) is considered here. In this recipe, the critical gradient occurs when the AE linear growth rate, driven mainly by the EP gradient, exceeds the ion temperature gradient (ITG) or trapped electron mode (TEM) growth rate, driven by the thermal plasma gradient, at the same toroidal mode number (n) as the AE peak growth, well below the ITG/TEMmore » peak n. This linear recipe for the critical gradient is validated against the critical gradient determined from far more expensive local nonlinear simulations in the gyrokinetic code GYRO, as identified by the point of transport runaway when all driving gradients are held fixed. The reduced linear model is extended to include the stabilization from equilibrium E×B velocity shear. The nonlinear verification unambiguously endorses one of two alternative recipes proposed in Ref. 1: the EP-driven AE growth rate should be determined with rather than without added thermal plasma drive.« less

Nonlinear verification of a linear critical gradient model for energetic particle transport by Alfven eigenmodes

DOE PAGES

Bass, Eric M.; Waltz, R. E.

2017-12-08

Here, a “stiff transport” critical gradient model of energetic particle (EP) transport by EPdriven Alfven eigenmodes (AEs) is verified against local nonlinear gyrokinetic simulations of a well-studied beam-heated DIII-D discharge 146102. A greatly simplifying linear “recipe” for the limiting EP-density gradient (critical gradient) is considered here. In this recipe, the critical gradient occurs when the AE linear growth rate, driven mainly by the EP gradient, exceeds the ion temperature gradient (ITG) or trapped electron mode (TEM) growth rate, driven by the thermal plasma gradient, at the same toroidal mode number (n) as the AE peak growth, well below the ITG/TEMmore » peak n. This linear recipe for the critical gradient is validated against the critical gradient determined from far more expensive local nonlinear simulations in the gyrokinetic code GYRO, as identified by the point of transport runaway when all driving gradients are held fixed. The reduced linear model is extended to include the stabilization from equilibrium E×B velocity shear. The nonlinear verification unambiguously endorses one of two alternative recipes proposed in Ref. 1: the EP-driven AE growth rate should be determined with rather than without added thermal plasma drive.« less

Phase space representation of neutron monitor count rate and atmospheric electric field in relation to solar activity in cycles 21 and 22.

PubMed

Silva, H G; Lopes, I

Heliospheric modulation of galactic cosmic rays links solar cycle activity with neutron monitor count rate on earth. A less direct relation holds between neutron monitor count rate and atmospheric electric field because different atmospheric processes, including fluctuations in the ionosphere, are involved. Although a full quantitative model is still lacking, this link is supported by solid statistical evidence. Thus, a connection between the solar cycle activity and atmospheric electric field is expected. To gain a deeper insight into these relations, sunspot area (NOAA, USA), neutron monitor count rate (Climax, Colorado, USA), and atmospheric electric field (Lisbon, Portugal) are presented here in a phase space representation. The period considered covers two solar cycles (21, 22) and extends from 1978 to 1990. Two solar maxima were observed in this dataset, one in 1979 and another in 1989, as well as one solar minimum in 1986. Two main observations of the present study were: (1) similar short-term topological features of the phase space representations of the three variables, (2) a long-term phase space radius synchronization between the solar cycle activity, neutron monitor count rate, and potential gradient (confirmed by absolute correlation values above ~0.8). Finally, the methodology proposed here can be used for obtaining the relations between other atmospheric parameters (e.g., solar radiation) and solar cycle activity.

An Examination of Potential Causes of the Persistent Capillary Fringe Extension Observed During a Pumping Test in an Unconfined Aquifer

NASA Astrophysics Data System (ADS)

Bunn, M. I.; Jones, J.; Endres, A. L.

2008-12-01

Hydrogeologists quantify the properties of unconfined aquifers by analyzing the data from pumping tests. The most appropriate method of incorporating flow contributions from the vadose zone into these analyses has been the subject of debate for decades. Recently, a highly detailed data set was collected during a seven- day pumping test at CFB Borden, Ontario (Bevan et al., 2005) which has allowed a close examination of the vadose zone response to pumping. Water table drawdown was monitored using pressure transducers in 11 monitoring wells, while moisture profiles were collected 19 times during the 7-day test using neutron logging. The Borden aquifer system is quite homogeneous, and numerical simulations using the variably saturated model InHM resulted in excellent reproduction of the observed hydraulic head drawdowns. Conversely, the simulated moisture profiles correlated poorly with neutron-logging-derived observed profiles. Specifically, the field results show delayed drawdown in the vadose zone, resulting in a persistent and significant extension of the capillary fringe, with the shape of the moisture profile remaining constant through the transition zone. Numerical simulations using various forms of the capillary pressure-saturation relationship with reasonable parameter sets were unable produce the extension. Neutron moisture profiles were selected from three locations (3, 5, and 15 m radial distance from the pumping well) at which an adjacent shallow deep piezometer pair could be used to accurately estimate water table location. Using this data in conjunction with the inverse modeling tool PEST, a set of van Genuchten capillary pressure-saturation parameters was generated to match each observed moisture profile. Horizontal and vertical hydraulic gradients and flow rates at the water table were generated using model output and compared to the fitted parameters. The van Genuchten parameter n was found to have significant scatter in both profile location and observation time when compared to any of the modeled results. The van Genuchten parameter alpha was found to vary linearly as a function of horizontal hydraulic gradient; further the results from all observation locations and times were found to follow the same linear relationship. The likely effects of consolidation, entrapped air, heterogeneity, and hydraulic gradients on the observed moisture profile were also evaluated. Results indicate a need for further investigation into the applicability of laboratory derived steady-state water retention curves for field scale simulations.

43 CFR 3253.11 - Must I notify BLM when I have completed my exploration operations?

Code of Federal Regulations, 2011 CFR

2011-10-01

... (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL...) Complete any geophysical exploration operations; (b) Complete the drilling of temperature gradient well(s... gradient well; and (d) Plug shot holes and reclaim all exploration sites. ...

Assessment of Ionospheric Gradient Impacts on Ground-Based Augmentation System (GBAS) Data in Guangdong Province, China

PubMed Central

Wang, Zhipeng; Wang, Shujing; Zhu, Yanbo; Xin, Pumin

2017-01-01

Ionospheric delay is one of the largest and most variable sources of error for Ground-Based Augmentation System (GBAS) users because inospheric activity is unpredictable. Under normal conditions, GBAS eliminates ionospheric delays, but during extreme ionospheric storms, GBAS users and GBAS ground facilities may experience different ionospheric delays, leading to considerable differential errors and threatening the safety of users. Therefore, ionospheric monitoring and assessment are important parts of GBAS integrity monitoring. To study the effects of the ionosphere on the GBAS of Guangdong Province, China, GPS data collected from 65 reference stations were processed using the improved “Simple Truth” algorithm. In addition, the ionospheric characteristics of Guangdong Province were calculated and an ionospheric threat model was established. Finally, we evaluated the influence of the standard deviation and maximum ionospheric gradient on GBAS. The results show that, under normal ionospheric conditions, the vertical protection level of GBAS was increased by 0.8 m for the largest over bound σvig (sigma of vertical ionospheric gradient), and in the case of the maximum ionospheric gradient conditions, the differential correction error may reach 5 m. From an airworthiness perspective, when the satellite is at a low elevation, this interference does not cause airworthiness risks, but when the satellite is at a high elevation, this interference can cause airworthiness risks. PMID:29019953

Assessment of Ionospheric Gradient Impacts on Ground-Based Augmentation System (GBAS) Data in Guangdong Province, China.

PubMed

Wang, Zhipeng; Wang, Shujing; Zhu, Yanbo; Xin, Pumin

2017-10-11

Ionospheric delay is one of the largest and most variable sources of error for Ground-Based Augmentation System (GBAS) users because inospheric activity is unpredictable. Under normal conditions, GBAS eliminates ionospheric delays, but during extreme ionospheric storms, GBAS users and GBAS ground facilities may experience different ionospheric delays, leading to considerable differential errors and threatening the safety of users. Therefore, ionospheric monitoring and assessment are important parts of GBAS integrity monitoring. To study the effects of the ionosphere on the GBAS of Guangdong Province, China, GPS data collected from 65 reference stations were processed using the improved "Simple Truth" algorithm. In addition, the ionospheric characteristics of Guangdong Province were calculated and an ionospheric threat model was established. Finally, we evaluated the influence of the standard deviation and maximum ionospheric gradient on GBAS. The results show that, under normal ionospheric conditions, the vertical protection level of GBAS was increased by 0.8 m for the largest over bound σ v i g (sigma of vertical ionospheric gradient), and in the case of the maximum ionospheric gradient conditions, the differential correction error may reach 5 m. From an airworthiness perspective, when the satellite is at a low elevation, this interference does not cause airworthiness risks, but when the satellite is at a high elevation, this interference can cause airworthiness risks.

Proposition of stair climb of a drop using chemical wettability gradient

NASA Astrophysics Data System (ADS)

Seerha, Prabh P. S.; Kumar, Parmod; Das, Arup K.; Mitra, Sushanta K.

2017-07-01

We propose a passive technique for a drop to climb along the staircase textured surface using chemical wettability gradients. The stair structure, droplet configuration, and contact angle gradient are modeled using Lagrangian smoothed particle hydrodynamics. The stair climb efficiency of the droplet is found to be a function of wettability gradient strength. Using analytical balance of actuation and resistive forces across droplets, physical reasons behind stair climbing are established and influencing parameters are identified. Evolution of the droplet shape along with the advancing and the receding contact angles is presented from where instantaneous actuation and hysteresis forces are calculated. Using history of Lagrangian particles, circulation at the foot of stairs and progressing development of the advancing drop front are monitored. Higher efficiency in stair climbing in the case of a bigger sized drop than smaller one is obtained from simulation results and realized from force balance. Difficulty in climbing steeper stairs is also demonstrated to delineate the effect of gravitational pull against the actuation force due to the wettability gradient.

Mobile Monitoring of Methane During and After the Aliso Canyon Natural Gas Leak

NASA Astrophysics Data System (ADS)

Polidori, A.; Pikelnaya, O.; Low, J.; Wimmer, R.; Zhou, Q.

2016-12-01

The Aliso Canyon gas leak was discovered inside the SoCalGas (SCG) facility on October 23, 2015. This incident represented the worst natural gas leak in the US history, and spurred a number of odor nuisance complaints from local residents. The community of Porter Ranch, located directly south of the SCG Aliso Canyon facility, was the most affected by the leak although complaints have been also reported in other neighboring communities of the San Fernando Valley. Therefore, monitoring of air quality was and remains crucial for measuring the impact of methane emissions from this leak and assessing the well-being of all residents. As the main local air quality agency for this area, South Coast Air Quality Management District (SCAQMD) organized a set of monitoring activities in response to the leak. Since December 21, 2015 SCAQMD has been conducting mobile survey measurements in and around Porter Ranch to characterize methane levels and concentration gradients within the community. For this purpose, a fast-response optical methane analyzer (LI-COR 7700) and a Global Positioning System (GPS) were mounted on top of a hybrid vehicle and driven around Porter Ranch and other surrounding areas. Following the permanent seal of the leaking well on February 18, 2016 mobile measurements have also been expanded to inside the Aliso Canyon SCG facility. During this presentation we will describe the experimental setup designed for mobile methane surveys and the monitoring strategy used for this study. We will discuss the main results of our mobile measurements including long-term methane trends since the end of the leak.

Experimental measurements of the SP response to concentration and temperature gradients in sandstones with application to subsurface geophysical monitoring

NASA Astrophysics Data System (ADS)

Leinov, E.; Jackson, M. D.

2014-09-01

Exclusion-diffusion potentials arising from temperature gradients are widely neglected in self-potential (SP) surveys, despite the ubiquitous presence of temperature gradients in subsurface settings such as volcanoes and hot springs, geothermal fields, and oil reservoirs during production via water or steam injection. Likewise, with the exception of borehole SP logging, exclusion-diffusion potentials arising from concentration gradients are also neglected or, at best, it is assumed that the diffusion potential dominates. To better interpret these SP sources requires well-constrained measurements of the various coupling terms. We report measurements of thermoelectric and electrochemical exclusion-diffusion potentials across sandstones saturated with NaCl brine and find that electrode effects can dominate the measured voltage. After correcting for these, we find that Hittorf transport numbers are the same within experimental error regardless of whether ion transport occurs in response to temperature or concentration gradients over the range of NaCl concentration investigated that is typical of natural systems. Diffusion potentials dominate only if the pore throat radius is more than approximately 4000 times larger than the diffuse layer thickness. In fine-grained sandstones with small pore throat diameter, this condition is likely to be met only if the saturating brine is of relatively high salinity; thus, in many cases of interest to earth scientists, exclusion-diffusion potentials will comprise significant contributions from both ionic diffusion through, and ionic exclusion from, the pore space of the rock. However, in coarse-grained sandstones, or sandstones saturated with high-salinity brine, exclusion-diffusion potentials can be described using end-member models in which ionic exclusion is neglected. Exclusion-diffusion potentials in sandstones depend upon pore size and salinity in a complex way: they may be positive, negative, or zero depending upon sandstone rock texture (expressed here by the pore radius r) and salinity.

Use of the pH sensitive fluorescence probe pyranine to monitor internal pH changes in Escherichia coli membrane vesicles.

PubMed

Damiano, E; Bassilana, M; Rigaud, J L; Leblanc, G

1984-01-23

Measurements of the fluorescent properties of 8-hydroxy-1,3,6-pyrenetrisulfonate (pyranine) enclosed within the internal space of Escherichia coli membrane vesicles enable recordings and quantitative analysis of: (i) changes in intravesicular pH taking place during oxidation of electron donors by the membrane respiratory chain; (ii) transient alkalization of the internal aqueous space resulting from the creation of outwardly directed acetate diffusion gradients across the vesicular membrane. Quantitation of the fluorescence variations recorded during the creation of transmembrane acetate gradients shows a close correspondence between the measured shifts in internal pH value and those expected from the amplitude of the imposed acetate gradients.

Meteorological effects on long-range outdoor sound propagation

NASA Technical Reports Server (NTRS)

Klug, Helmut

1990-01-01

Measurements of sound propagation over distances up to 1000 m were carried out with an impulse sound source offering reproducible, short time signals. Temperature and wind speed at several heights were monitored simultaneously; the meteorological data are used to determine the sound speed gradients according to the Monin-Obukhov similarity theory. The sound speed profile is compared to a corresponding prediction, gained through the measured travel time difference between direct and ground reflected pulse (which depends on the sound speed gradient). Positive sound speed gradients cause bending of the sound rays towards the ground yielding enhanced sound pressure levels. The measured meteorological effects on sound propagation are discussed and illustrated by ray tracing methods.

Event-Based Analysis of Rainfall-Runoff Response to Assess Wetland-Stream Interaction in the Prairie Pothole Region

NASA Astrophysics Data System (ADS)

Haque, M. A.; Ross, C.; Schmall, A.; Bansah, S.; Ali, G.

2016-12-01

Process-based understanding of wetland response to precipitation is needed to quantify the extent to which non-floodplain wetlands - such as Prairie potholes - generate flow and transmit that flow to nearby streams. While measuring wetland-stream (W-S) interaction is difficult, it is possible to infer it by examining hysteresis characteristics between wetland and stream stage during individual precipitation events. Hence, to evaluate W-S interaction, 10 intact and 10 altered/lost potholes were selected for study; they are located in Broughton's Creek Watershed (Manitoba, Canada) on both sides of a 5 km creek reach. Stilling wells (i.e., above ground wells) were deployed in the intact and altered wetlands to monitor surface water level fluctuations while water table wells were drilled below drainage ditches to a depth of 1 m to monitor shallow groundwater fluctuations. All stilling wells and water table wells were equipped with capacitance water level loggers to monitor fluctuations in surface water and shallow groundwater every 15 minutes. In 2013 (normal year) and 2014 (wet year), 15+ precipitation events were identified and scatter plots of wetland (x-axis) versus stream (y-axis) stage were built to identify W-S hysteretic dynamics. Initial data analysis reveals that in dry antecedent conditions, intact and altered wetlands show clockwise W-S relations, while drained wetlands show anticlockwise W-S hysteresis. However, in wetter antecedent conditions, all wetland types show anticlockwise hysteresis. Future analysis will target the identification of thresholds in antecedent moisture conditions that determine significant changes in event wetland response characteristics (e.g., the delay between the start of rainfall and stream stage, the maximum water level rise in each wetland during each event, the delay between the start of rainfall and peak wetland stage) as well as hysteresis properties (e.g., gradient and area of the hysteresis loop).

Acoustical experiment of yogurt fermentation process.

PubMed

Ogasawara, H; Mizutani, K; Ohbuchi, T; Nakamura, T

2006-12-22

One of the important factors through food manufacturing is hygienic management. Thus, food manufactures prove their hygienic activities by taking certifications like a Hazard Analysis and Critical Control Point (HACCP). This concept also applies to food monitoring. Acoustical measurements have advantage for other measurement in food monitoring because they make it possible to measure with noncontact and nondestructive. We tried to monitor lactic fermentation of yogurt by a probing sensor using a pair of acoustic transducers. Temperature of the solution changes by the reaction heat of fermentation. Consequently the sound velocity propagated through the solution also changes depending on the temperature. At the same time, the solution change its phase from liquid to gel. The transducers usage in the solution indicates the change of the temperature as the change of the phase difference between two transducers. The acoustic method has advantages of nondestructive measurement that reduces contamination of food product by measuring instrument. The sensor was inserted into milk with lactic acid bacterial stain of 19 degrees C and monitored phase retardation of propagated acoustic wave and its temperature with thermocouples in the mild. The monitoring result of fermentation from milk to Caspian Sea yogurt by the acoustic transducers with the frequency of 3.7 MHz started to show gradient change in temperature caused by reaction heat of fermentation but stop the gradient change at the end although the temperature still change. The gradient change stopped its change because of phase change from liquid to gel. The present method will be able to measure indirectly by setting transducers outside of the measuring object. This noncontact sensing method will have great advantage of reduces risk of food contamination from measuring instrument because the measurement probes are set out of fermentation reactor or food containers. Our proposed method will contribute to the hygienization for the food manufacture industry.

The challenge of monitoring the cryosphere in alpine environments: Prepare the present for the future

NASA Astrophysics Data System (ADS)

Fischer, Andrea; Helfricht, Kay; Seiser, Bernd; Stocker-Waldhuber, Martin; Hartl, Lea; Wiesenegger, Hans

2017-04-01

Understanding the interaction of mountain glaciers and permafrost with weather and climate is essential for the interpretation of past states of the cryosphere in terms of climate change. Most of the glaciers and rock glaciers in Eastern Alpine terrain are subject to strong gradients in climatic forcing, and the persistence of these gradients under past climatic conditions is, more or less, unknown. Thus a key challenge of monitoring the cryosphere is to define the demands on a monitoring strategy for capturing essential processes and their potential changes. For example, the effects of orographic precipitation and local shading vary with general circulation patterns and the amount of solar radiation during the melt(ing) season. Recent investigations based on the Austrian glacier inventories have shown that glacier distribution is closely linked to topography and climatic situation, and that these two parameters imply also different sensitivities of the specific glaciers to progressing climate change. This leads to the need to develop a monitoring system capturing past, but also fairly unknown future ensembles of climatic state and sensitivities. As a first step, the Austrian glacier monitoring network has been analyzed from the beginning of the records onwards. Today's monitoring network bears the imprints of past research interests, but also past funding policies and personal/institutional engagements. As a limitation for long term monitoring in general, today's monitoring strategies have to cope with being restricted to these historical commitments to preserve the length of the time series, but at the same time expanding the measurements to fulfil present and future scientific and societal demands. The decision on cryospheric benchmark sites has an additional uncertainty: the ongoing disintegration of glaciers, their increasing debris cover as well as the potential low ice content and relatively unknown reaction of rock glaciers in the course of climate change, limits the number of potential candidates for future monitoring drastically. In the light of these developments, sample sizes are a critical question for reliable monitoring, together with strategies for coping with changing monitoring sites and composition of time series. As a first step, the Austrian monitoring network has been analyzed from 1891 onwards. Past changes evident from the glacier inventories capturing all glaciers have been compared to the subsamples of glaciers monitored for length change, mass balance and ice flow velocities. The results show that for capturing the full bandwidth of regional changes, glacier inventories are necessary. Without the analysis of larger scale changes, the interpretation of records with very low sample sizes, such as mass balance or length change, has a high uncertainty level. For specific research or monitoring purposes, for example, the development of runoff master sites with all types of monitoring techniques improve the certainty of the spatial extrapolations of local records or the interpretation of volume changes. The challenge of preparing the present network for the future requires a thorough analysis of potential future developments to be able to switch sites with a common observation period necessary to investigate the different sensitivities.

Six-hourly time series of horizontal troposphere gradients in VLBI analyis

NASA Astrophysics Data System (ADS)

Landskron, Daniel; Hofmeister, Armin; Mayer, David; Böhm, Johannes

2016-04-01

Consideration of horizontal gradients is indispensable for high-precision VLBI and GNSS analysis. As a rule of thumb, all observations below 15 degrees elevation need to be corrected for the influence of azimuthal asymmetry on the delay times, which is mainly a product of the non-spherical shape of the atmosphere and ever-changing weather conditions. Based on the well-known gradient estimation model by Chen and Herring (1997), we developed an augmented gradient model with additional parameters which are determined from ray-traced delays for the complete history of VLBI observations. As input to the ray-tracer, we used operational and re-analysis data from the European Centre for Medium-Range Weather Forecasts. Finally, we applied those a priori gradient parameters to VLBI analysis along with other empirical gradient models and assessed their impact on baseline length repeatabilities as well as on celestial and terrestrial reference frames.

Evaluation of multiple tracer methods to estimate low groundwater flow velocities.

PubMed

Reimus, Paul W; Arnold, Bill W

2017-04-01

Four different tracer methods were used to estimate groundwater flow velocity at a multiple-well site in the saturated alluvium south of Yucca Mountain, Nevada: (1) two single-well tracer tests with different rest or "shut-in" periods, (2) a cross-hole tracer test with an extended flow interruption, (3) a comparison of two tracer decay curves in an injection borehole with and without pumping of a downgradient well, and (4) a natural-gradient tracer test. Such tracer methods are potentially very useful for estimating groundwater velocities when hydraulic gradients are flat (and hence uncertain) and also when water level and hydraulic conductivity data are sparse, both of which were the case at this test location. The purpose of the study was to evaluate the first three methods for their ability to provide reasonable estimates of relatively low groundwater flow velocities in such low-hydraulic-gradient environments. The natural-gradient method is generally considered to be the most robust and direct method, so it was used to provide a "ground truth" velocity estimate. However, this method usually requires several wells, so it is often not practical in systems with large depths to groundwater and correspondingly high well installation costs. The fact that a successful natural gradient test was conducted at the test location offered a unique opportunity to compare the flow velocity estimates obtained by the more easily deployed and lower risk methods with the ground-truth natural-gradient method. The groundwater flow velocity estimates from the four methods agreed very well with each other, suggesting that the first three methods all provided reasonably good estimates of groundwater flow velocity at the site. The advantages and disadvantages of the different methods, as well as some of the uncertainties associated with them are discussed. Published by Elsevier B.V.

UAV hyperspectral and lidar data analysis for vegetation applications

NASA Astrophysics Data System (ADS)

Sankey, Temuulen; Sankey, Joel; Donager, Jonathon

2017-04-01

High spatial and spectral resolution remote sensing data are critically needed to classify forest vegetation and measure their structure at the level of individual species and canopies. Here we test high-resolution lidar and hyperspectral data from unmanned aerial vehicles (UAV) and demonstrate a lidar-hyperspectral image fusion method in treated and control forests with varying tree density and canopy cover as well as in an ecotone with a gradient of vegetation and topography in northern Arizona, USA. The fusion performs better (88% overall accuracy) than either data type alone, particularly for species with similar spectral signature, but different canopy sizes. The lidar data provides estimates of individual tree height (R2=0.90; RMSE=2.3m) and crown diameter (R2=0.72; RMSE=0.71m) as well as total tree canopy cover (R2=0.87; RMSE=9.5%) and tree density (R2=0.77; RMSE=0.69 trees/cell) in 10 m cells across thin only, burn only, thin-and-burn, and control treatments, where tree cover and density ranged between 22-50% and 1-3.5 trees/cell, respectively. The lidar data also produces high accuracy DEM (R2=0.95; RMSE=0.43m). The lidar and hyperspectral sensors and methods demonstrated here can be widely applied across a gradient of vegetation and topography for monitoring ecosystem changes.

MODELING TRANSPORT IN THE DOWN GRADIENT PORTION OF THE 200-PO-1 OPERABLE UNIT AT THE HANFORD SITE

DOE Office of Scientific and Technical Information (OSTI.GOV)

MEHTA S; ALY AH; MILLER CW

2009-12-03

Remedial Investigations are underway for the 200-PO-l Operable Unit (OU) at the U.S. Department of Energy's Hanford Site in Washington State. To support the baseline risk assessment and evaluation of remedial alternatives, fate and transport modeling is being conducted to predict the future concentration of contaminants of potential concern in the 200-PO-1 OU. This study focuses on modeling the 'down gradient' transport of those contaminants that migrate beyond the 3-D model domain selected for performing detailed 'source area' modeling within the 200-PO-1 OU. The down gradient portion is defined as that region of the 200-PO-1 OU that is generally outsidemore » the 200 Area (considered 'source area') of the Hanford Site. A 1-D transport model is developed for performing down gradient contaminant fate and transport modeling. The 1-D transport model is deemed adequate based on the inferred transport pathway of tritium in the past and the observation that most of the contaminant mass remains at or near the water table within the unconfined aquifer of the Hanford Formation and the Cold-Creek/Pre-Missoula Gravel unit. The Pipe Pathway feature of the GoldSim software is used to perform the calculations. The Pipe Pathway uses a Laplace transform approach to provide analytical solutions to a broad range of advection-dominated mass transport systems involving one-dimensional advection, longitudinal dispersion, retardation, decay and ingrowth, and exchanges with immobile storage zones. Based on the historical concentration distribution data for the extensive tritium plume in this area, three Pipe Pathways are deemed adequate for modeling transport of contaminants. Each of these three Pipe Pathways is discretized into several zones, based on the saturated thickness variation in the unconfined aquifer and the location of monitoring wells used for risk assessment calculation. The mass fluxes of contaminants predicted to exit the source area model domain are used as an input to the down gradient model, while the flow velocities applied are based on the present-day hydraulic gradients and estimation of hydraulic conductivity in the unconfined aquifer. The results of the calculation indicate that the future concentrations of contaminants of potential concern in the down gradient portion of the 200-PO-1 OU declines with time and distance.« less

The Researches on Reasonable Well Spacing of Gas Wells in Deep and low Permeability Gas Reservoirs

NASA Astrophysics Data System (ADS)

Bei, Yu Bei; Hui, Li; Lin, Li Dong

2018-06-01

This Gs64 gas reservoir is a condensate gas reservoir which is relatively integrated with low porosity and low permeability found in Dagang Oilfield in recent years. The condensate content is as high as 610g/m3. At present, there are few reports about the well spacing of similar gas reservoirs at home and abroad. Therefore, determining the reasonable well spacing of the gas reservoir is important for ensuring the optimal development effect and economic benefit of the gas field development. This paper discusses the reasonable well spacing of the deep and low permeability gas reservoir from the aspects of percolation mechanics, gas reservoir engineering and numerical simulation. considering there exist the start-up pressure gradient in percolation process of low permeability gas reservoir, this paper combined with productivity equation under starting pressure gradient, established the formula of gas well spacing with the formation pressure and start-up pressure gradient. The calculation formula of starting pressure gradient and well spacing of gas wells. Adopting various methods to calculate values of gas reservoir spacing are close to well testing' radius, so the calculation method is reliable, which is very important for the determination of reasonable well spacing in low permeability gas reservoirs.

Team Cappadocia Design for MAGIC 2010 (The ASELSAN Team)

DTIC Science & Technology

2010-11-01

Also in another screen, OCU displays tactical information using 3D Geographical Information System (GIS) for enhanced situational awareness. OCU...number of local textural features such as mean and standard deviation of image intensity and gradient, Zernike moments, Haralick features...information for situational awareness on the 3D GIS tactical map and the other monitor is used for UGV status monitoring, command and control. Tactical

Dynamics of groundwater-surface water interactions in urban streams

NASA Astrophysics Data System (ADS)

Musolff, A.; Schmidt, C.; Fleckenstein, J. H.

2010-12-01

In industrialized countries the majority of streams and rivers have been subject to changes in the hydrological regime and alteration of the channel morphology. Urban streams are typically characterized by “flashier” hydrographs as a result of more direct runoff from impervious surfaces. Channel structure and complexity are often impaired compared to pristine streams. As a consequence the potential for bedform-driven water flow in the streambed is reduced. The downward transport of oxygen by advective flow in the streambed is known to be of great ecological importance for the hyporheic macro and micro fauna and facilitates nutrient cycling and the degradation of organic pollutants. We studied the dynamics of groundwater-surface water exchange of two anthropogenically impacted streams in urban areas to examine the effects of variable hydrologic boundary conditions on water flux and redox conditions in the streambed. The first stream is fed by groundwater as well as storm-water from a large industrial area. Here, we monitored the variability of vertical hydraulic gradients, streambed temperature and redox conditions in the streambed over the course of 5 months. The second stream is frequently polluted by combined sewer overflows (CSO) from an urban watershed. Here, we measured the vertical hydraulic gradients, streambed temperature and electrical conductivity (EC) in the stream, the streambed and in the adjacent aquifer. Both streams are characterized by strong variations in hydraulic gradients due to the dynamic hydrographs as well as the variations in total head in the shallow aquifer. Therefore, magnitude and direction of water flux through the streambed changed significantly over time. At the first site long-term variations of redox conditions in the shallow streambed (0.1 m) were related to the direction of water fluxes. Downward water flow resulted in increased redox potentials. However, the high short-term variability of redox conditions could not be directly attributed to changes in the hydraulic conditions. At the second site, increased EC in the shallow aquifer was related to seasonally losing conditions (associated with low water tables in summer) and the resulting groundwater recharge. Sudden increases in stream stage due to rain events and subsequent CSO resulted in altered streambed water fluxes, as evidenced by the disturbance of vertical streambed temperature profiles down to a depth of 0.3 m. Both, short-term and long-term variations in hydraulic gradients between the stream, the streambed and the groundwater were found to influence the magnitude and direction of water fluxes. Flashy flow events influence the water flux in the streambed very rapidly. However, changes in redox potential in the streambed require losing conditions over time scales longer than the duration of a typical high flow event. As a consequence, the complexity of water exchange in the streambed should be carefully monitored, both in space and time. Our results indicate that variable hydraulic gradients may induce intense exchange fluxes between the stream and streambed in urban streams and may compensate some of the negative consequences of degraded channels with limited bedform-driven flow.

Enhancing early detection of exotic pests in agricultural and forest ecosystems using an urban-gradient framework.

PubMed

Colunga-Garcia, Manuel; Magarey, Roger A; Haack, Robert A; Gage, Stuart H; Qi, Jiaquo

2010-03-01

Urban areas are hubs of international transport and therefore are major gateways for exotic pests. Applying an urban gradient to analyze this pathway could provide insight into the ecological processes involved in human-mediated invasions. We defined an urban gradient for agricultural and forest ecosystems in the contiguous United States to (1) assess whether ecosystems nearer more urbanized areas were at greater risk of invasion, and (2) apply this knowledge to enhance early detection of exotic pests. We defined the gradient using the tonnage of imported products in adjacent urban areas and their distance to nearby agricultural or forest land. County-level detection reports for 39 exotic agricultural and forest pests of major economic importance were used to characterize invasions along the gradient. We found that counties with more exotic pests were nearer the urban end of the gradient. Assuming that the exotic species we analyzed represent typical invaders, then early detection efforts directed at 21-26% of U.S. agricultural and forest land would likely be able to detect 70% of invaded counties and 90% of the selected species. Applying an urban-gradient framework to current monitoring strategies should enhance early detection efforts of exotic pests, facilitating optimization in allocating resources to areas at greater risk of future invasions.

Arbitrary magnetic field gradient waveform correction using an impulse response based pre-equalization technique.

PubMed

Goora, Frédéric G; Colpitts, Bruce G; Balcom, Bruce J

2014-01-01

The time-varying magnetic fields used in magnetic resonance applications result in the induction of eddy currents on conductive structures in the vicinity of both the sample under investigation and the gradient coils. These eddy currents typically result in undesired degradations of image quality for MRI applications. Their ubiquitous nature has resulted in the development of various approaches to characterize and minimize their impact on image quality. This paper outlines a method that utilizes the magnetic field gradient waveform monitor method to directly measure the temporal evolution of the magnetic field gradient from a step-like input function and extracts the system impulse response. With the basic assumption that the gradient system is sufficiently linear and time invariant to permit system theory analysis, the impulse response is used to determine a pre-equalized (optimized) input waveform that provides a desired gradient response at the output of the system. An algorithm has been developed that calculates a pre-equalized waveform that may be accurately reproduced by the amplifier (is physically realizable) and accounts for system limitations including system bandwidth, amplifier slew rate capabilities, and noise inherent in the initial measurement. Significant improvements in magnetic field gradient waveform fidelity after pre-equalization have been realized and are summarized. Copyright © 2013 Elsevier Inc. All rights reserved.

Geohydrology and potential for upward movement of saline water in the Cocoa well field, East Orange County, Florida

USGS Publications Warehouse

Phelps, G.G.; Schiffer, D.M.

1996-01-01

The Floridan aquifer system, an approximately 2,000-foot thick sequence of Eocene-age limestone and dolomite, is the main source of water supply in central Florida. Hydraulic conductivity is different in strata of different lithology and is the basis for separating the aquifer system into the Upper Floridan aquifer, a middle semi- confining unit, and the Lower Floridan aquifer. The coastal city of Cocoa withdraws about 26 million gallons of water per day from the Upper Floridan aquifer from a well field in east Orange County, about 25 miles inland. About 60 million gallons per day are withdrawn from the Upper Floridan aquifer and 56 million gallons per day from the Lower Floridan aquifer in the Orlando area, about 15 miles west of the Cocoa well field. Wells drilled in the Cocoa well field from 1955-61 yielded water with chloride concentrations ranging from 25-55 milligrams per liter. Soon after the wells were put in service, chloride concentrations increased; therefore, new wells were drilled further inland. Chloride concen- trations in water from many of the new wells also have increased. Possible sources of saline water are lateral movement of relict seawater in the Upper Floridan aquifer from the east, regional upconing of saline water from the Lower Floridan aquifer or underlying older rocks, or localized upward movement of saline water through fractures. Several test wells were drilled to provide information about chloride concentration changes with depth and to monitor changes with time, including a multi-zone well drilled in 1965 (well C) and two wells drilled in the 1990's (wells R and S). Chloride concentrations have increased in the zone pumped by the supply wells (the upper 500 feet of the aquifer) and in the 1,351-1,357-foot deep zone of well C, but not in the two intervening zones. This indicates that the source of saline water is located laterally, rather than vertically, from the pumped zone in the area of well C. The potential for upward movement of saline water depends on the direction of the vertical hydraulic gradient and on the vertical hydraulic conductivity of the aquifer. A series of aquifer tests was run in 1993-94 and existing water-level and water-quality data were analyzed to evaluate the potential for upward movement of saline water in the well field. The transmissivity of the upper 500 feet of the aquifer is about 100,000 feet squared per day (the horizontal hydraulic conductivity is about 200 feet per day) and the storage coefficient is about 2x10 -4. Horizontal hydraulic conductivities determined from slug tests of the three deepest zones of well C ranged from 20-50 feet per day. Vertical hydraulic conductivities probably do not exceed 0.05 feet per day. The vertical hydraulic gradient is determined by comparing water levels in the various zones, but because of density differences, unadjusted water levels in the deepest zone investigated cannot be directly compared to water levels in the overlying freshwater zones. The difference between environmental-water heads (adjusted for density differences) in the saline-water zone of well C and the overlying freshwater zone were calculated from measured water levels for the period 1966 to 1994. During most of this time period, the gradient was downward, indicating that saline water did not move upward. Upconing of saline water probably is not taking place in the center and western part of the well field, based on the low vertical hydraulic conductivity values estimated for the middle semi-confining unit, the generally downward vertical hydraulic gradient, and the constant chloride concentrations in the intermediate zones of well C. However, there is no information about the extent of the zone of low vertical hydraulic conductivity gradient in the eastern part of the well field. Thus, increased chloride concentrations in supply wells in the eastern part of the well field could be caused either by lateral movement of saline water from the east, or by upwar

The Northwest Geysers EGS Demonstration Project, California

DOE PAGES

Garcia, Julio; Hartline, Craig; Walters, Mark; ...

2015-09-04

Our project goal is to demonstrate the feasibility of stimulating a deep high-temperature reservoir (HTR) (up to 400 °C, 750 °F). There were two previously abandoned wells, Prati State 31 (PS-31) and Prati 32 (P-32), reopened and deepened to be used as an injection and production doublet to stimulate the HTR. The deepened portions of both wells have conductive temperature gradients of 10 °F/100 ft (182 °C/km), produce connate native fluids and magmatic gas, and the rocks were isotopically unexchanged by meteoric water. The ambient temperature meteoric water injected into these hot dry rocks has evidently created a permeability volume of several cubic kilometers asmore » determined by seismic monitoring. Preliminary isotopic analyses of the injected and produced water indicate that 50–75% of the steam from the created EGS reservoir is injection-derived.« less

Physical aging and its influence on the reliability of network epoxies and epoxy-matrix composites

NASA Technical Reports Server (NTRS)

Heinemann, K.

1983-01-01

The matrix-dominated physical and mechanical properties of a carbon fiber reinforced epoxy composite and a neat epoxy resin were found to be affected by sub-Tg annealing in nitrogen and dark atmosphere. Postcured specimens of Thornel 300 carbon-fiber/Fiberite 934 epoxy as well as Fiberite 934 epoxy resin were quenched from above Tg and given annealing at 140 C, 110 C, or 80 C, for time up to one-hundred thousand minutes. No weight loss was observed during annealing at these temperatures. Significant variations were found in density, modulus, hardness, damping, moisture absorption ability, thermal expansivity. Moisture-epoxy interactious were also studied. The kinetics of aging as well as the molecular aggregation during this densification process were monitored by differential scanning calorimetry, dynamic mechanical analysis, density gradient column, microhardness tester, Instron, and solid-state nuclear magnetic resonance spectroscopy.

In Situ Monitoring of Crystal Growth Using MEPHISTO, Mission STS 87-Program USMP-4: Experimental Analysis

NASA Technical Reports Server (NTRS)

Abbaschian, Reza; Chen, F.; Mileham, J. R.; deGroh, H., III; Timchenko, V.; Leonardi, E.; deVahlDavis, G.; Coriell, S.; Cambon, G.

1999-01-01

This report summarizes the results of the In situ Monitoring of Crystal Growth Using MEPHISTO (Material por l'Etude des Phenomenes Interessant de la Solidification sur Terre et en Orbite) experiment on USMP-4. The report includes microstructural and compositional data obtained during the first year of the post flight analysis, as well as numerical simulation of the flight experiment. Additional analyses are being continued and will be reported in the near future. The experiments utilized MEPHISTO hardware to study the solidification and melting behavior of bismuth alloyed with 1 at% tin. The experiments involved repeated melting and solidification of three samples, each approximately 90 cm long and 6mm in diameter. Half of each sample also included a 2 mm. diameter growth capillary, to assist in the formation of single grain inside. One sample provided the Seebeck voltage generated during melting and freezing processes. Another one provided temperature data and Peltier pulsed demarcation of the interface shape for post flight analysis. The third sample provided resistance and velocity measurements, as well as additional thermal data. The third sample was also quenched at the end of the mission to preserve the interface composition for post flight determination. A total of more than 45cm of directionally solidified alloy were directionally solidified at the end of the flight for post mission structural and compositional characterization. Metallurgical analysis of the samples has shown that the interfacial kinetics play a key role in controlling the morphological stability of faceted alloys. Substantial differences were observed in the Seebeck signal between the ground-based experiments and the space-based experiments. The temperature gradient in the liquid for the ground-based experiments was also significantly lower than the temperature gradient in the liquid for the space-based experiments. Both of these observations indicate significant influence of liquid convection for the ground-based experiments.

Temperature gradients in a portion of Michigan: a review of the usefulness of data from the AAPG geothermal survey of North America

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vaught, T.L.

1980-08-01

Temperature gradient data derived from drill holes in an east-west zone through the center of the southern peninsula of Michigan are analyzed. The purpose of this work is to investigate possible problems in utilizing the American Association of Petroleum Geologists data base. Michigan was chosen because a review of that State's geothermal potential shows inconsistencies between gradients from shallow wells and nearby deeper wells and because the geology of the State is relativey simple. The structure and stratigraphy are discussed because an understanding of Michigan basin geology makes it easier to predict the influence of lithology on the basin's geothermalmore » gradients. Explanations for elevated gradients are reviewed. (MHR)« less

A comprehensive assessment of ionospheric gradients observed in Ecuador during 2013 and 2014 for ground based augmentation systems

NASA Astrophysics Data System (ADS)

Sánchez-Naranjo, S.; Rincón, W.; Ramos-Pollán, R.; González, F. A.; Soley, S.

2017-04-01

Ground Based Augmentation Systems GBAS provide differential corrections to approaching and landing aircrafts in the vicinities of an airport. The ionosphere can introduce an error not accountable by those differential corrections, and a threat model for the Conterminous United States region CONUS was developed in order to consider the highest gradients measured. This study presents the first extensive analysis of ionospheric gradients for Ecuador, from data fully covering 2013 and 2014 collected by their national Global Navigation Satellite System GNSS monitoring network (REGME). In this work it is applied an automated methodology adapted for low latitudes for processing data from dual frequency receivers networks, by considering data from all available days in the date range of the study regardless the geomagnetic indices values. The events found above the CONUS threat model occurred during days of nominal geomagnetic indices, confirming: (1) the higher bounds required for an ionospheric threat model for Ecuador, and (2) that geomagnetic indices are not enough to indicate relevant ionospheric anomalies in low latitude regions, reinforcing the necessity of a continuous monitoring of ionosphere. As additional contribution, the events database is published online, making it available to other researchers.

Linearly chirped fiber Bragg grating response to thermal gradient: from bench tests to the real-time assessment during in vivo laser ablations of biological tissue.

PubMed

Saccomandi, Paola; Varalda, Ambra; Gassino, Riccardo; Tosi, Daniele; Massaroni, Carlo; Caponero, Michele A; Pop, Raoul; Korganbayev, Sanzhar; Perrone, Guido; Diana, Michele; Vallan, Alberto; Costamagna, Guido; Marescaux, Jacques; Schena, Emiliano

2017-09-01

The response of a fiber optic sensor [linearly chirped fiber Bragg grating (LCFBG)] to a linear thermal gradient applied on its sensing length (i.e., 1.5 cm) has been investigated. After these bench tests, we assessed their feasibility for temperature monitoring during thermal tumor treatment. In particular, we performed experiments during ex vivo laser ablation (LA) in pig liver and in vivo thermal ablation in animal models (pigs). We investigated the following: (i) the relationship between the full width at half maximum of the LCFBG spectrum and the temperature difference among the extremities of the LCFBG and (ii) the relationship between the mean spectrum wavelength and the mean temperature acting on the LCFBG sensing area. These relationships showed a linear trend during both bench tests and LA in animal models. Thermal sensitivity was significant although different values were found with regards to bench tests and animal experiments. The linear trend and significant sensitivity allow hypothesizing a future use of this kind of sensor to monitor both temperature gradient and mean temperature within a tissue undergoing thermal treatment. (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Electrokinetic Stabilisation Method of Soft Clay in Pure System using Electrokinetic Geosynthetic Electrode

NASA Astrophysics Data System (ADS)

Azhar, A. T. S.; Jefferson, I.; Madun, A.; Abidin, M. H. Z.; Rogers, C. D. F.

2018-04-01

Electrokinetic stabilisation (EKS) method has the ability to solve the problems of soft highly compressibility soil. This study will present the results from an experimental study of EKS on soft soils using inactive kaolinite clay, inert electrode and distilled water (DW) as a pure system mechanism before any chemical stabilisers being used in this research. Therefore, this will provide a baseline study to improve the efficiency of EKS approach. The test model was using inert electrode of Electrokinetic Geosythentic (EKG) developed at the Newcastle University to apply a constant voltage gradient of 50 V/m across a soil sample approximately 400 mm. Distilled water was used at the pore electrolyte fluid compartments supplied under zero hydraulic gradient conditions for the periods of 3, 7 and 14 days. Throughout the monitoring, physical and chemical characteristics were measured. Results from the monitoring data, physical and chemical properties of the pure system showed the development of pH gradient, the changes of electrical conductivity and chemical concentrations with regards to the distance from anode and treatment periods due to the electrochemical effects even though there was no chemical stabilisers were introduced or released from the degradation of electrodes.

Process tool monitoring and matching using interferometry technique

NASA Astrophysics Data System (ADS)

Anberg, Doug; Owen, David M.; Mileham, Jeffrey; Lee, Byoung-Ho; Bouche, Eric

2016-03-01

The semiconductor industry makes dramatic device technology changes over short time periods. As the semiconductor industry advances towards to the 10 nm device node, more precise management and control of processing tools has become a significant manufacturing challenge. Some processes require multiple tool sets and some tools have multiple chambers for mass production. Tool and chamber matching has become a critical consideration for meeting today's manufacturing requirements. Additionally, process tools and chamber conditions have to be monitored to ensure uniform process performance across the tool and chamber fleet. There are many parameters for managing and monitoring tools and chambers. Particle defect monitoring is a well-known and established example where defect inspection tools can directly detect particles on the wafer surface. However, leading edge processes are driving the need to also monitor invisible defects, i.e. stress, contamination, etc., because some device failures cannot be directly correlated with traditional visualized defect maps or other known sources. Some failure maps show the same signatures as stress or contamination maps, which implies correlation to device performance or yield. In this paper we present process tool monitoring and matching using an interferometry technique. There are many types of interferometry techniques used for various process monitoring applications. We use a Coherent Gradient Sensing (CGS) interferometer which is self-referencing and enables high throughput measurements. Using this technique, we can quickly measure the topography of an entire wafer surface and obtain stress and displacement data from the topography measurement. For improved tool and chamber matching and reduced device failure, wafer stress measurements can be implemented as a regular tool or chamber monitoring test for either unpatterned or patterned wafers as a good criteria for improved process stability.

Explaining social class differences in depression and well-being.

PubMed

Stansfeld, S A; Head, J; Marmot, M G

1998-01-01

Work characteristics, including skill discretion and decision authority, explain most of the socioeconomic status gradient in well-being and depression in middle-aged British civil servants from the Whitehall II Study, London. Social support explained about one-third of the gradient, life events and material difficulties less than one-third. Socioeconomic status was measured by employment grade. Work characteristics were based on the Karasek model, social support was measured by the Close Persons Questionnaire, depression by the General Health Questionnaire and well-being by the Affect Balance Scale. Despite a small contribution from social selective factors measured by upward mobility, the psychosocial work environment explained most of the cross-sectional socioeconomic status gradient in well-being and depression.

SU-E-T-354: Efficient and Enhanced QA Testing of Linear Accelerators Using a Real-Time Beam Monitor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jung, J; Farrokhkish, M; Norrlinger, B

2015-06-15

Purpose: To investigate the feasibility of performing routine QA tests of linear accelerators (Linac) using the Integral Quality Monitoring (IQM) system. The system, consisting of a 1-D sensitivity gradient large area ion-chamber mounted at the collimator, allows automatic collection and analysis of beam data. Methods: The IQM was investigated to perform several QA constancy tests, similar to those recommended by AAPM TG142, of a Linac including: beam output, MLC calibration, beam symmetry, relative dose factor (RDF), dose linearity, output as a function of gantry angle and dose rate. All measurements by the IQM system accompanied a reference measurement using amore » conventional dosimetry system and were performed on an Elekta Infinity Linac with Agility MLC. The MLC calibration check is done using a Picket-Fence type 2×10cm{sup 2} field positioned at different off-axis locations along the chamber gradient. Beam symmetry constancy values are established by signals from an 4×4cm{sup 2} aperture located at various off-axis positions; the sensitivity of the test was determined by the changes in the signals in response to a tilt in the beam. The data for various square field sizes were used to develop a functional relationship with RDF. Results: The IQM tracked the beam output well within 1% of the reference ion-chamber readings. The Picket-Fence type field test detected a 1mm shift error of one MLC bank. The system was able to detect 2.5% or greater beam asymmetry. The IQM results for all other QA tests were found to agree with the reference values to within 0.5%. Conclusion: It was demonstrated that the IQM system can effectively monitor the Linac performance parameters for the purpose of routine QA constancy tests. With minimum user interactions a comprehensive set of tests can be performed efficiently, allowing frequent monitoring of the Linac. The presenting author’s salary is funded by the manufacturer of the QA device. All the other authors have financial interests with the commercialization of this QA device.« less

Reconnaissance of surface-water quality in the North Platte Natural Resources District, western Nebraska, 1993

USGS Publications Warehouse

Steele, G.V.; Cannia, J.C.

1997-01-01

In 1993, the U.S. Geological Survey and the North Platte Natural Resources District began a 3-year study to determine the geohydrology and water quality of the North Platte River alluvial aquifer near Oshkosh, Garden County, Nebraska. The objectives of the study were to determine the geohydrologic properties of the North Platte River alluvial aquifer, to establish a well network for long- term monitoring of concentrations of agricultural chemicals including nitrate and herbicides, and to establish baseline concentrations of major ions in the ground water. To meet these objectives, monitor wells were installed at 11 sites near Oshkosh. The geohydrologic properties of the aquifer were estimated from water-level measurements at selected irrigation wells located in the study area and short- term constant-discharge aquifer tests at two monitor wells. Water samples were collected bimonthly and analyzed for specific conductance, pH, water temperature, dissolved oxygen, and nutrients including dissolved nitrate. Samples were collected semiannually for analysis of major ions, and annually for triazine and acetamide herbicides. Evaluation of the aquifer-test data indicates the hydraulic conductivities of the North Platte River alluvial aquifer range between 169 and 184 feet per day and transmissivities ranged from 12,700 to 26,700 feet-squared per day. The average specific yield for the alluvial aquifer, based on the two aquifer tests, was 0.2. Additional hydrologic data for the alluvial aquifer include a horizontal gradient of about 0.002 foot per foot and estimated ground- water flow velocities of about 0.1 to 1.8 feet per day. Evaluation of the water-quality data indicates that nitrate concentrations exceed the U.S. Environmental Protection Agency's (USEPA) Maximum Contamination Level of 10 milligrams per liter for drinking water in areas to the east and west of Oshkosh. In these areas, nitrate concentrations generally are continuing to rise. West of Oshkosh the highest concentrations are now exceeding 50 milligrams per liter. With the exception of one sample, nitrate concentrations exceeding the Maximum Contamination Level were not detected in three wells used to monitor the ground water flowing into and out of the study area, nor in a monitor well located near a municipal well. Results of the study also indicate that an influx of water from Lost Creek Valley, north of the study area, may be mixing with ground water near Oshkosh and diluting concentrations of nitrate.

A New Approach for Identifying Ionospheric Gradients in the Context of the Gagan System

NASA Astrophysics Data System (ADS)

Kudala, Ravi Chandra

2012-10-01

The Indian Space Research Organization and the Airports Authority of India are jointly implementing the Global Positioning System (GPS) aided GEO Augmented Navigation (GAGAN) system in order to meet the following required navigation performance (RNP) parameters: integrity, continuity, accuracy, and availability (for aircraft operations). Such a system provides the user with orbit, clock, and ionospheric corrections in addition to ranging signals via the geostationary earth orbit satellite (GEOSAT). The equatorial ionization anomaly (EIA), due to rapid non-uniform electron-ion recombination that persists on the Indian subcontinent, causes ionospheric gradients. Ionospheric gradients represent the most severe threat to high-integrity differential GNSS systems such as GAGAN. In order to ensure integrity under conditions of an ionospheric storm, the following three objectives must be met: careful monitoring, error bounding, and sophisticated storm-front modeling. The first objective is met by continuously tracking data due to storms, and, on quiet days, determining precise estimates of the threat parameters from reference monitoring stations. The second objective is met by quantifying the above estimates of threat parameters due to storms through maximum and minimum typical thresholds. In the context GAGAN, this work proposes a new method for identifying ionospheric gradients, in addition to determining an appropriate upper bound, in order to sufficiently understand error during storm days. Initially, carrier phase data of the GAGAN network from Indian TEC stations for both storm and quiet days was used for estimating ionospheric spatial and temporal gradients (the vertical ionospheric gradient (σVIG) and the rate of the TEC index (ROTI), respectively) in multiple viewing directions. Along similar lines, using the carrier to noise ratio (C/N0) for the same data, the carrier to noise ratio index (σCNRI) was derived. Subsequently, the one-toone relationship between σVIG and σCNRI was examined. High values of σVIG were determined for strong noise signals and corresponded to minimal σCNRI, indicating poor phase estimations and, in turn, an erroneous location. On the other hand, low values of σVIG were produced for weak noise signals and corresponded to maximum σCNRI, indicating strong phase estimations and, in turn, accurate locations. In other words, if a gradient persists in the line of sight direction of GEOSAT for aviation users, the down link L- band signal itself becomes erroneous. As a result, the en-route aviation user fails to receive a SBAS correction message leading to deprivation for the main objective of GAGAN. On the other hand, since the proposed approach enhances the receivers of both the aviation user and the reference monitoring station in terms of their performance, based on σCNRI, the integrity of SBAS messages themselves can be analyzed and considered for forward corrections.

Screening for microplastic particles in plankton samples: How to integrate marine litter assessment into existing monitoring programs?

PubMed

Gorokhova, Elena

2015-10-15

Microplastics (MPs) are a newly recognized type of environmental pollution in aquatic systems; however no monitoring of these contaminants is conducted, mostly due to the lack of routine quantification. In the net samples collected with a 90-μm WP2 net, pelagic MP abundance was quantified by light microscopy and evaluated as a function of inshore-offshore gradient, depth, and season; the same samples were used for zooplankton analysis. The MP abundance was ∼10(2)-10(4)particlesm(-3), with no significant inshore-offshore gradient during summer but increasing offshore in winter. MP abundance in deeper layers was positively affected by zooplankton abundance in the upper layers and significantly lower during winter compared to summer. These findings indicate heterogeneity of MP distribution due to biotic and abiotic factors and suggest that samples collected for other purposes can be used for quantification of MPs in the Baltic Sea, thus facilitating integration of MP assessment into existing monitoring schemes. Copyright © 2015 Elsevier Ltd. All rights reserved.

Measuring small compartment dimensions by probing diffusion dynamics via Non-uniform Oscillating-Gradient Spin-Echo (NOGSE) NMR.

PubMed

Shemesh, Noam; Alvarez, Gonzalo A; Frydman, Lucio

2013-12-01

Noninvasive measurements of microstructure in materials, cells, and in biological tissues, constitute a unique capability of gradient-assisted NMR. Diffusion-diffraction MR approaches pioneered by Callaghan demonstrated this ability; Oscillating-Gradient Spin-Echo (OGSE) methodologies tackle the demanding gradient amplitudes required for observing diffraction patterns by utilizing constant-frequency oscillating gradient pairs that probe the diffusion spectrum, D(ω). Here we present a new class of diffusion MR experiments, termed Non-uniform Oscillating-Gradient Spin-Echo (NOGSE), which dynamically probe multiple frequencies of the diffusion spectral density at once, thus affording direct microstructural information on the compartment's dimension. The NOGSE methodology applies N constant-amplitude gradient oscillations; N-1 of these oscillations are spaced by a characteristic time x, followed by a single gradient oscillation characterized by a time y, such that the diffusion dynamics is probed while keeping (N-1)x+y≡TNOGSE constant. These constant-time, fixed-gradient-amplitude, multi-frequency attributes render NOGSE particularly useful for probing small compartment dimensions with relatively weak gradients - alleviating difficulties associated with probing D(ω) frequency-by-frequency or with varying relaxation weightings, as in other diffusion-monitoring experiments. Analytical descriptions of the NOGSE signal are given, and the sequence's ability to extract small compartment sizes with a sensitivity towards length to the sixth power, is demonstrated using a microstructural phantom. Excellent agreement between theory and experiments was evidenced even upon applying weak gradient amplitudes. An MR imaging version of NOGSE was also implemented in ex vivo pig spinal cords and mouse brains, affording maps based on compartment sizes. The effects of size distributions on NOGSE are also briefly analyzed. Copyright © 2013 Elsevier Inc. All rights reserved.

High-resolution fast temperature mapping of a gas turbine combustor simulator with femtosecond infrared laser written fiber Bragg gratings

NASA Astrophysics Data System (ADS)

Walker, Robert B.; Yun, Sangsig; Ding, Huimin; Charbonneau, Michel; Coulas, David; Ramachandran, Nanthan; Mihailov, Stephen J.

2017-02-01

Femtosecond infrared (fs-IR) written fiber Bragg gratings (FBGs), have demonstrated great potential for extreme sensing. Such conditions are inherent to the advanced gas turbine engines under development to reduce greenhouse gas emissions; and the ability to measure temperature gradients in these harsh environments is currently limited by the lack of sensors and controls capable of withstanding the high temperature, pressure and corrosive conditions present. This paper discusses fabrication and deployment of several fs-IR written FBG arrays, for monitoring the sidewall and exhaust temperature gradients of a gas turbine combustor simulator. Results include: contour plots of measured temperature gradients contrasted with thermocouple data, discussion of deployment strategies and comments on reliability.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, James Christopher; Rennie, John Alan; Toevs, James Waldo

The introduction points out that radiation backgrounds fluctuate across very short distances: factors include geology, soil composition, altitude, building structures, topography, and other manmade structures; and asphalt and concrete can vary significantly over short distances. Brief descriptions are given of the detection system, experimental setup, and background variation measurements. It is concluded that positive and negative gradients can greatly reduce the detection sensitivity of an MDS: negative gradients create opportunities for false negatives (nondetection), and positive gradients create a potentially unacceptable FAR (above 1%); the location of use for mobile detection is important to understand; spectroscopic systems provide more informationmore » for screening out false alarms and may be preferred for mobile use; and mobile monitor testing at LANL accounts for expected variations in the background.« less

Dengue Vectors and their Spatial Distribution

PubMed Central

Higa, Yukiko

2011-01-01

The distribution of dengue vectors, Ae. aegypti and Ae. albopictus, is affected by climatic factors. In addition, since their life cycles are well adapted to the human environment, environmental changes resulting from human activity such as urbanization exert a great impact on vector distribution. The different responses of Ae. aegypti and Ae albopictus to various environments result in a difference in spatial distribution along north-south and urban-rural gradients, and between the indoors and outdoors. In the north-south gradient, climate associated with survival is an important factor in spatial distribution. In the urban-rural gradient, different distribution reflects a difference in adult niches and is modified by geographic and human factors. The direct response of the two species to the environment around houses is related to different spatial distribution indoors and outdoors. Dengue viruses circulate mainly between human and vector mosquitoes, and the vector presence is a limiting factor of transmission. Therefore, spatial distribution of dengue vectors is a significant concern in the epidemiology of the disease. Current technologies such as GIS, satellite imagery and statistical models allow researchers to predict the spatial distribution of vectors in the changing environment. Although it is difficult to confirm the actual effect of environmental and climate changes on vector abundance and vector-borne diseases, environmental changes caused by humans and human behavioral changes due to climate change can be expected to exert an impact on dengue vectors. Longitudinal monitoring of dengue vectors and viruses is therefore necessary. PMID:22500133

Temperature mapping of laser-induced hyperthermia in an ocular phantom using magnetic resonance thermography.

PubMed

Maswadi, Saher M; Dodd, Stephen J; Gao, Jia-Hong; Glickman, Randolph D

2004-01-01

Laser-induced heating in an ocular phantom is measured with magnetic resonance thermography (MRT) using temperature-dependent phase changes in proton resonance frequency. The ocular phantom contains a layer of melanosomes isolated from bovine retinal pigment epithelium. The phantom is heated by the 806-nm output of a continuous wave diode laser with an irradiance of 2.4 to 21.6 W/cm2 in a beam radius of 0.8 or 2.4 mm, depending on the experiment. MRT is performed with a 2 T magnet, and a two-turn, 6-cm-diam, circular radio frequency coil. Two-dimensional temperature gradients are measured within the plane of the melanin layer, as well as normal to it, with a temperature resolution of 1 degrees C or better. The temperature gradients extending within the melanin layer are broader than those orthogonal to the layer, consistent with the higher optical absorption and consequent heating in the melanin. The temperature gradients in the phantom measured by MRT closely approximate the predictions of a classical heat diffusion model. Three-dimensional temperature maps with a spatial resolution of 0.25 mm in all directions are also made. Although the temporal resolution is limited in the prototype system (22.9 s for a single image "slice"), improvements in future implementations are likely. These results indicate that MRT has sufficient spatial and temperature resolution to monitor target tissue temperature during transpupillary thermotherapy in the human eye.

Dissipation of the Proton Electrochemical Potential in Intact Chloroplasts (II. The pH Gradient Monitored by Cytochrome f Reduction Kinetics).

PubMed Central

Nishio, J. N.; Whitmarsh, J.

1993-01-01

The potency of various uncouplers for collapsing the light-induced pH gradient across thylakoid membranes in intact chloroplasts was investigated by time-resolved optical spectroscopy. The thylakoid transmembrane pH gradient ([delta]pH) was monitored indirectly by measuring the rate of cytochrome (Cyt) f reduction following a light flash of sufficient duration to create a sizable [delta]pH. The results show that the rate of Cyt f reduction is controlled in part by the internal pH of the thylakoid inner aqueous space. At pH values from 6.5 to 8.0, the Cyt f reduction rate was maximal, whereas at lower pH values from 6.5 to 5.5 the reduction rate decreased to 25% of the maximal rate. The ability of three uncouplers, nigericin, carbonylcyanide m-chlorophenylhydrazone, and gramicidin, to accelerate the rate of Cyt f reduction was determined for intact chloroplasts isolated from spinach (Spinacia oleracea). The efficacy of the uncouplers for collapsing the [delta]pH was determined using the empirical relationship between the [delta]pH and the Cyt f reduction rate. For intact chloroplasts, nigericin was the most effective uncoupler, followed by carbonylcyanide m-chlorophenylhydrazone, which interacted strongly with bovine serum albumin. Gramicidin D, even at high gramicidin:chlorophyll ratios, did not completely collapse the pH gradient, probably because it partitions in the envelope membranes and does not enter the intact chloroplast. PMID:12231669

Synchrotron X-ray measurement techniques for thermal barrier coated cylindrical samples under thermal gradients.

PubMed

Siddiqui, Sanna F; Knipe, Kevin; Manero, Albert; Meid, Carla; Wischek, Janine; Okasinski, John; Almer, Jonathan; Karlsson, Anette M; Bartsch, Marion; Raghavan, Seetha

2013-08-01

Measurement techniques to obtain accurate in situ synchrotron strain measurements of thermal barrier coating systems (TBCs) applied to hollow cylindrical specimens are presented in this work. The Electron Beam Physical Vapor Deposition coated specimens with internal cooling were designed to achieve realistic temperature gradients over the TBC coated material such as that occurring in the turbine blades of aeroengines. Effects of the circular cross section on the x-ray diffraction (XRD) measurements in the various layers, including the thermally grown oxide, are investigated using high-energy synchrotron x-rays. Multiple approaches for beam penetration including collection, tangential, and normal to the layers, along with variations in collection parameters are compared for their ability to attain high-resolution XRD data from the internal layers. This study displays the ability to monitor in situ, the response of the internal layers within the TBC, while implementing a thermal gradient across the thickness of the coated sample. The thermal setup maintained coating surface temperatures in the range of operating conditions, while monitoring the substrate cooling, for a controlled thermal gradient. Through variation in measurement location and beam parameters, sufficient intensities are obtained from the internal layers which can be used for depth resolved strain measurements. Results are used to establish the various techniques for obtaining XRD measurements through multi-layered coating systems and their outcomes will pave the way towards goals in achieving realistic in situ testing of these coatings.

Synchrotron X-ray measurement techniques for thermal barrier coated cylindrical samples under thermal gradients

NASA Astrophysics Data System (ADS)

Siddiqui, Sanna F.; Knipe, Kevin; Manero, Albert; Meid, Carla; Wischek, Janine; Okasinski, John; Almer, Jonathan; Karlsson, Anette M.; Bartsch, Marion; Raghavan, Seetha

2013-08-01

Measurement techniques to obtain accurate in situ synchrotron strain measurements of thermal barrier coating systems (TBCs) applied to hollow cylindrical specimens are presented in this work. The Electron Beam Physical Vapor Deposition coated specimens with internal cooling were designed to achieve realistic temperature gradients over the TBC coated material such as that occurring in the turbine blades of aeroengines. Effects of the circular cross section on the x-ray diffraction (XRD) measurements in the various layers, including the thermally grown oxide, are investigated using high-energy synchrotron x-rays. Multiple approaches for beam penetration including collection, tangential, and normal to the layers, along with variations in collection parameters are compared for their ability to attain high-resolution XRD data from the internal layers. This study displays the ability to monitor in situ, the response of the internal layers within the TBC, while implementing a thermal gradient across the thickness of the coated sample. The thermal setup maintained coating surface temperatures in the range of operating conditions, while monitoring the substrate cooling, for a controlled thermal gradient. Through variation in measurement location and beam parameters, sufficient intensities are obtained from the internal layers which can be used for depth resolved strain measurements. Results are used to establish the various techniques for obtaining XRD measurements through multi-layered coating systems and their outcomes will pave the way towards goals in achieving realistic in situ testing of these coatings.

Water-chemistry and chloride fluctuations in the Upper Floridan Aquifer in the Port Royal Sound area, South Carolina, 1917-93

USGS Publications Warehouse

Landmeyer, J.E.; Belval, D.L.

1996-01-01

Withdrawal of water from the Upper Floridan aquifer south of Port Royal Sound in Beaufort and Jasper Counties, South Carolina, has lowered water levels and reversed the hydraulic gradient beneath Hilton Head Island, South Carolina. Ground water that had previously discharged at the Sound is now being deflected southwest, toward withdrawals located near the city of Savannah, Georgia, and the island of Hilton Head. The reversal of this hydraulic gradient and the decline of water levels have caused saltwater in the Upper Floridan aquifer north of Port Royal Sound to begin moving southwest, toward water-supply wells for the town of Hilton Head and toward industries pumping ground water near Savannah. Analytical results from ground-water samples collected from wells in the Upper Floridan aquifer beneath and adjacent to Port Royal Sound show two plumes in the aquifer with chloride concentrations above the drinking- water standard. One plume of high chloride concentration extends slightly south of the theoretical predevelopment location of the steady- state freshwater-saltwater interface as indicated by numerical modeling. The other plume is present beneath the town of Port Royal, where the upper confining unit above the Upper Floridan aquifer is thin or absent. In these areas, the decline in water levels caused by ground-water withdrawals may have made it possible for water from tidal creeks to enter the Upper Floridan aquifer. Many wells completed in the upper permeable zone of the Upper Floridan aquifer show a distinct specific- conductance profile. One non-producing, monitoring well on Hilton Head Island (BFT-1810) was selected to depict a worst-case scenario to examine the short- and long-term water-chemistry and chloride fluctuations in the aquifer. Specific conductance was monitored at depths of 170, 190, and 200 feet below the top of the well casing. The specific conductance measured in 1987 ranged from approximately 450 microsiemens per centimeter near the top of the Upper Floridan aquifer to 1,500 microsiemens per centimeter near the lower, less permeable zone. Short-term fluctuations in conductance were measured at each probe and were found to be related to water-level fluctuations in the well caused by tidal cycles. The conductance varied regularly up to 100 microsiemens per centimeter, with an increasing time lag between high and low tides and low and high specific conductance for progressively shallower depths. Well BFT-1810 was monitored for specific conductance and water levels from October 1987 through September 1993. Specific conductance at the 170-foot probe showed little long-term change, while the 190- and the 200-foot probes showed long-term increases to approximately 4,000 and 10,000 microsiemens per centimeter, respectively. This well is located closest to one of the two plumes of saltwater delineated in the Upper Floridan aquifer, and the long-term chloride increases are a result of the movement of saltwater in the Upper Floridan aquifer toward Hilton Head Island under the influence of regional ground-water withdrawals.

Hydrogeology and physical characteristics of water samples at the Red River aluminum site, Stamps, Arkansas, April 2001

USGS Publications Warehouse

Czarnecki, John B.; Stanton, Gregory P.; Freiwald, David A.

2001-01-01

The Red River Aluminum site near Stamps, Arkansas, contains waste piles of salt cake and metal byproducts from the smelting of aluminum. The waste piles are subjected to about 50 inches of rainfall a year, resulting in the dissolution of the salts and metal. To assess the potential threat to underlying ground-water resources at the site, its hydrogeology was characterized by measuring water levels and field parameters of water quality in 23 wells and at 2 surface-water sites. Seventeen of these monitor wells were constructed at various depths for this study to allow for the separate characterization of the shallow and deep ground-water systems, the calculation of vertical gradients, and the collection of water samples at different depths within the flow system. Lithologic descriptions from drill-hole cuttings and geophysical logs indicate the presence of interbedded sands, gravels, silts, and clays to depths of 65 feet. The regionally important Sparta aquifer underlies the site. Water levels in shallow wells indicate radial flow away from the salt-cake pile located near the center of the site. Flow in the deep system is to the west and southwest toward Bodcau Creek. Water-level data from eight piezometer nests indicate a downward hydraulic gradient from the shallow to deep systems across the site. Values of specific conductance (an indicator of dissolved salts) ranged from 215 to 196,200 microsiemens per centimeter and indicate that saline waters are being transported horizontally and vertically downward away from the site

Regional recovery of the disturbing gravitational potential by inverting satellite gravitational gradients

NASA Astrophysics Data System (ADS)

Pitoňák, Martin; Šprlák, Michal; Hamáčková, Eliška; Novák, Pavel

2016-04-01

Regional recovery of the disturbing gravitational potential in the area of Central Europe from satellite gravitational gradients data is discussed in this contribution. The disturbing gravitational potential is obtained by inverting surface integral formulas which transform the disturbing gravitational potential onto disturbing gravitational gradients in the spherical local north-oriented frame. Two numerical approaches that solve the inverse problem are considered. In the first approach, the integral formulas are rigorously decomposed into two parts, that is, the effects of the gradient data within near and distant zones. While the effect of the near zone data is sought as an inverse problem, the effect of the distant zone data is synthesized from the global gravitational model GGM05S using spectral weights given by truncation error coefficients up to the degree 150. In the second approach, a reference gravitational field up to the degree 180 is applied to reduce and smooth measured gravitational gradients. In both cases we recovered the disturbing gravitational potential from each of the four well-measured gravitational gradients of the GOCE satellite separately as well as from their combination. Obtained results are compared with the EGM2008, DIR-r2, TIM-r2 and SPW-r2 global gravitational models. The best fit was achieved for EGM2008 and the second approach combining all four well-measured gravitational gradients with rms of 1.231 m2 s-2.

Multi-gradient drilling method and system

DOEpatents

Maurer, William C.; Medley, Jr., George H.; McDonald, William J.

2003-01-01

A multi-gradient system for drilling a well bore from a surface location into a seabed includes an injector for injecting buoyant substantially incompressible articles into a column of drilling fluid associated with the well bore. Preferably, the substantially incompressible articles comprises hollow substantially spherical bodies.

Bacterial Population Changes in a Membrane Bioreactor for Graywater Treatment Monitored by Denaturing Gradient Gel Electrophoretic Analysis of 16S rRNA Gene Fragments

PubMed Central

Stamper, David M.; Walch, Marianne; Jacobs, Rachel N.

2003-01-01

The bacterial population of a graywater treatment system was monitored over the course of 100 days, along with several wastewater biochemical parameters. The graywater treatment system employed an 1,800-liter membrane bioreactor (MBR) to process the waste, with essentially 100% recycling of the biomass. Graywater feed consisting of 10% galley water and 90% laundry water, selected to approximate the graywater composition on board U.S. Navy ships, was collected offsite. Five-day biological oxygen demand (BOD5), oils and greases (O/G), nitrogen, and phosphorus were monitored in the feed and were found to vary greatly day to day. Changes in the bacterial population were monitored by PCR amplification of region 332 to 518 (Escherichia coli numbering) of the 16S rRNA gene and denaturing gradient gel electrophoresis (DGGE) analysis of the resultant PCR products. DGGE analysis indicated a diverse and unstable bacterial population throughout the 100-day period, with spikes in feed strength causing significant changes in community structure. Long-term similarity between the communities was 0 to 25%, depending on the method of analysis. In spite of the unstable bacterial population, the MBR system was able to meet effluent quality parameters approximately 90% of the time. PMID:12571004

Bacterial population changes in a membrane bioreactor for graywater treatment monitored by denaturing gradient gel electrophoretic analysis of 16S rRNA gene fragments.

PubMed

Stamper, David M; Walch, Marianne; Jacobs, Rachel N

2003-02-01

The bacterial population of a graywater treatment system was monitored over the course of 100 days, along with several wastewater biochemical parameters. The graywater treatment system employed an 1,800-liter membrane bioreactor (MBR) to process the waste, with essentially 100% recycling of the biomass. Graywater feed consisting of 10% galley water and 90% laundry water, selected to approximate the graywater composition on board U.S. Navy ships, was collected offsite. Five-day biological oxygen demand (BOD(5)), oils and greases (O/G), nitrogen, and phosphorus were monitored in the feed and were found to vary greatly day to day. Changes in the bacterial population were monitored by PCR amplification of region 332 to 518 (Escherichia coli numbering) of the 16S rRNA gene and denaturing gradient gel electrophoresis (DGGE) analysis of the resultant PCR products. DGGE analysis indicated a diverse and unstable bacterial population throughout the 100-day period, with spikes in feed strength causing significant changes in community structure. Long-term similarity between the communities was 0 to 25%, depending on the method of analysis. In spite of the unstable bacterial population, the MBR system was able to meet effluent quality parameters approximately 90% of the time.

Geologic and geophysical data for wells drilled at Raft River Valley, Cassia County, Idaho, in 1977-1978 and data for wells drilled previously

USGS Publications Warehouse

Nathenson, Manuel; Urban, Thomas C.; Covington, Harry R.

2014-01-01

For purposes of defining the thermal anomaly for the geothermal system, temperature gradients are calculated over long depth intervals on the basis of the appearance of reasonable linear segments on a temperature versus plot depth.  Temperature versus depth data for some drill holes can be represented by a single gradient, whereas others require multiple gradients to match the data.  Data for some drill holes clearly reflect vertical flows of water in the formation surrounding the drill holes, and water velocities are calculated for these drill holes.  Within The Narrows area, temperature versus depth data show reversals at different depth in different drill holes.  In the main thermal area, temperatures in intermediate-depth drill holes vary approximately linearly but with very high values of temperature gradient.  Temperature gradients on a map of the area can be reasonable divided into a large area of regional gradients and smaller areas defining the thermal anomalies.

Floodplain-wide coupling of flooding and vegetation patterns in the Tonle Sap of the Mekong River

NASA Astrophysics Data System (ADS)

Arias, M. E.; Haberstroh, C.

2017-12-01

Floodplain vegetation is one of the prime drivers of ecosystem productivity, thus floodplain-wide monitoring is critical to ensure the well-being of these ecosystems and the important services they provide to riparian societies. Therefore, the objective of this presentation is to introduce a novel methodology to monitor long-term and large-scale patterns of rooted vegetation in seasonally inundated floodplains. We applied this methodology to an floodplain area of ac. 18,000 km2 in the Tonle Sap (Cambodia), a complex hydro-ecological system directly connected to the Mekong River. The overall hypothesis of this study is that floodplain vegetation condition is dictated by gradients of disturbance from the uplands and from the flood-pulse itself. We first demonstrate that spatial vegetation patterns represented by the normalized difference vegetation index (NDVI) during the dry season -when interference from cloud cover and partial inundation is minimal- correspond well to meaningful land use/land cover groups as well as canopy cover data collected in the field. Annual trends (2000-2016) in NDVI spatial distribution showed that the modality of dry season NDVI is largely governed by the magnitude of flooding in the antecedent hydrological year. Indeed, we found a significant relationship between flood duration -defined as the number of months annually a floodplain pixel remains flooded- and floodplain-wide NDVI. We also determined that ac. 115 km2 yr-1 of the highest quality vegetation, were replaced by fallow land during the period of study. This research has important insights on the main drivers of floodplain vegetation in the Tonle Sap, and the proposed methodology, using data from freely available worldwide satellite imagery (MODIS), promises to be an effective method to monitor ecosystem change in large floodplains across the world.

Groundwater chemistry in the vicinity of the Puna Geothermal Venture Power Plant, Hawai‘i, after two decades of production

USGS Publications Warehouse

Evans, W.C.; Bergfeld, D.; Sutton, A.J.; Lee, R.C.; Lorenson, T.D.

2015-01-01

We report chemical data for selected shallow wells and coastal springs that were sampled in 2014 to determine whether geothermal power production in the Puna area over the past two decades has affected the characteristics of regional groundwater. The samples were analyzed for major and minor chemical species, trace metals of environmental concern, stable isotopes of water, and two organic compounds (pentane and isopropanol) that are injected into the deep geothermal reservoir at the power plant. Isopropanol was not detected in any of the groundwaters; confirmed detection of pentane was restricted to one monitoring well near the power plant at a low concentration not indicative of source. Thus, neither organic compound linked geothermal operations to groundwater contamination, though chemical stability and transport velocity questions exist for both tracers. Based on our chemical analysis of geothermal fluid at the power plant and on many similar results from commercially analyzed samples, we could not show that geothermal constituents in the groundwaters we sampled came from the commercially developed reservoir. Our data are consistent with a long-held view that heat moves by conduction from the geothermal reservoir into shallow groundwaters through a zone of low permeability rock that blocks passage of geothermal water. The data do not rule out all impacts of geothermal production on groundwater. Removal of heat during production, for example, may be responsible for minor changes that have occurred in some groundwater over time, such as the decline in temperature of one monitoring well near the power plant. Such indirect impacts are much harder to assess, but point out the need for an ongoing groundwater monitoring program that should include the coastal springs down-gradient from the power plant.

Analysis of Correlation between Ionospheric Spatial Gradients and Space Weather Intensity under Nominal Conditions for Ground-Based Augmentation Systems

NASA Astrophysics Data System (ADS)

Lee, J.

2013-12-01

Ground-Based Augmentation Systems (GBAS) support aircraft precision approach and landing by providing differential GPS corrections to aviation users. For GBAS applications, most of ionospheric errors are removed by applying the differential corrections. However, ionospheric correction errors may exist due to ionosphere spatial decorrelation between GBAS ground facility and users. Thus, the standard deviation of ionosphere spatial decorrelation (σvig) is estimated and included in the computation of error bounds on user position solution. The σvig of 4mm/km, derived for the Conterminous United States (CONUS), bounds one-sigma ionospheric spatial gradients under nominal conditions (including active, but not stormy condition) with an adequate safety margin [1]. The conservatism residing in the current σvig by fixing it to a constant value for all non-stormy conditions could be mitigated by subdividing ionospheric conditions into several classes and using different σvig for each class. This new concept, real-time σvig adaptation, will be possible if the level of ionospheric activity can be well classified based on space weather intensity. This paper studies correlation between the statistics of nominal ionospheric spatial gradients and space weather indices. The analysis was carried out using two sets of data collected from Continuous Operating Reference Station (CORS) Network; 9 consecutive (nominal and ionospherically active) days in 2004 and 19 consecutive (relatively 'quiet') days in 2010. Precise ionospheric delay estimates are obtained using the simplified truth processing method and vertical ionospheric gradients are computed using the well-known 'station pair method' [2]. The remaining biases which include carrier-phase leveling errors and Inter-frequency Bias (IFB) calibration errors are reduced by applying linear slip detection thresholds. The σvig was inflated to overbound the distribution of vertical ionospheric gradients with the required confidence level. Using the daily maximum values of σvig, day-to-day variations of spatial gradients are compared to those of two space weather indices; Disturbance, Storm Time (Dst) index and Interplanetary Magnetic Field Bz (IMF Bz). The day-to-day variations of both space weather indices showed a good agreement with those of daily maximum σvig. The results demonstrate that ionospheric gradient statistics are highly correlated with space weather indices on nominal and off-nominal days. Further investigation on this relationship would facilitate prediction of upcoming ionospheric behavior based on space weather information and adjusting σvig in real time. Consequently it will improve GBAS availability by adding external information to operation. [1] Lee, J., S. Pullen, S. Datta-Barua, and P. Enge (2007), Assessment of ionosphere spatial decorrelation for GPS-based aircraft landing systems, J. Aircraft, 44(5), 1662-1669, doi:10.2514/1.28199. [2] Jung, S., and J. Lee (2012), Long-term ionospheric anomaly monitoring for ground based augmentation systems, Radio Sci., 47, RS4006, doi:10.1029/2012RS005016.

Assessment of ground-water contamination in the alluvial aquifer near West Point, Kentucky

USGS Publications Warehouse

Lyverse, M.A.; Unthank, M.D.

1988-01-01

Well inventories, water level measurements, groundwater quality samples, surface geophysical techniques (specifically, electromagnetic techniques), and test drilling were used to investigate the extent and sources of groundwater contamination in the alluvial aquifer near West Point, Kentucky. This aquifer serves as the principal source of drinking water for over 50,000 people. Groundwater flow in the alluvial aquifer is generally unconfined and moves in a northerly direction toward the Ohio River. Two large public supply well fields and numerous domestic wells are located in this natural flow path. High concentrations of chloride in groundwater have resulted in the abandonment of several public supply wells in the West Point areas. Chloride concentrations in water samples collected for this study were as high as 11,000 mg/L. Electromagnetic techniques indicated and test drilling later confirmed that the source of chloride in well waters was probably improperly plugged or unplugged, abandoned oil and gas exploration wells. The potential for chloride contamination of wells exists in the study area and is related to proximity to improperly abandoned oil and gas exploration wells and to gradients established by drawdowns associated with pumped wells. Periodic use of surface geophysical methods, in combination with added observation wells , could be used to monitor significant changes in groundwater quality related to chloride contamination. (USGS)

Application of Support Vector Machine to Forex Monitoring

NASA Astrophysics Data System (ADS)

Kamruzzaman, Joarder; Sarker, Ruhul A.

Previous studies have demonstrated superior performance of artificial neural network (ANN) based forex forecasting models over traditional regression models. This paper applies support vector machines to build a forecasting model from the historical data using six simple technical indicators and presents a comparison with an ANN based model trained by scaled conjugate gradient (SCG) learning algorithm. The models are evaluated and compared on the basis of five commonly used performance metrics that measure closeness of prediction as well as correctness in directional change. Forecasting results of six different currencies against Australian dollar reveal superior performance of SVM model using simple linear kernel over ANN-SCG model in terms of all the evaluation metrics. The effect of SVM parameter selection on prediction performance is also investigated and analyzed.

Mass Movement Susceptibility Mapping Using Satellite Optical Imagery Compared With INSAR Monitoring: Zigui County, Three Gorges Region, China

NASA Astrophysics Data System (ADS)

Kincal, Cem; Singleton, Andrew; Liu, Peng; Li, Zhenhong; Drummond, Jane; Hoey, Trevor; Muller, Jan-Peter; Qu, Wei; Zeng, Qiming; Zhang, Jingfa; Du, Peijun

2010-10-01

Mass movements on steep slopes are a major hazard to communities and infrastructure in the Three Gorges region, China. Developing susceptibility maps of mass movements is therefore very important in both current and future land use planning. This study employed satellite optical imagery and an ASTER GDEM (15 m) to derive various parameters (namely geology; slope gradient; proximity to drainage networks and proximity to lineaments) in order to create a GIS-based map of mass movement susceptibility. This map was then evaluated using highly accurate deformation signals processed using the Persistent Scatterer (PS) InSAR technique. Areas of high susceptibility correspond well to points of high subsidence, which provides a strong support of our susceptibility map.

Spatial Distribution of Circadian Clock Phase in Aging Cultures of Neurospora crassa1

PubMed Central

Dharmananda, Subhuti; Feldman, Jerry F.

1979-01-01

Neurospora crassa has been utilized extensively in the study of circadian clocks. Previously, the clock in this organism has been monitored by observing the morphological and biochemical changes occurring at the growing front of cultures grown on solid medium. A method has been developed for assaying the clock in regions of the culture behind the growing front, where no apparent morphological changes occur during the circadian cycle. Using this assay with Petri dish cultures that were 2 to 7 days old, the presence of a functional circadian clock not only at the growing front but in all other regions of the culture as well was demonstrated. Furthermore, the entire culture is not in the same phase, but shows a gradient of phases which is a function of the length of time the clock in a given part of the culture has been free-running. This gradient may be the result of a somewhat longer period of the oscillator behind the growing front compared to that at the growing front. The phase differences within a single culture of interconnected mycelium demonstrate the absence of total internal synchronization between adjacent regions of the hyphae under these conditions. PMID:16660855

Multi-Dimensional Quantum Tunneling and Transport Using the Density-Gradient Model

NASA Technical Reports Server (NTRS)

Biegel, Bryan A.; Yu, Zhi-Ping; Ancona, Mario; Rafferty, Conor; Saini, Subhash (Technical Monitor)

1999-01-01

We show that quantum effects are likely to significantly degrade the performance of MOSFETs (metal oxide semiconductor field effect transistor) as these devices are scaled below 100 nm channel length and 2 nm oxide thickness over the next decade. A general and computationally efficient electronic device model including quantum effects would allow us to monitor and mitigate these effects. Full quantum models are too expensive in multi-dimensions. Using a general but efficient PDE solver called PROPHET, we implemented the density-gradient (DG) quantum correction to the industry-dominant classical drift-diffusion (DD) model. The DG model efficiently includes quantum carrier profile smoothing and tunneling in multi-dimensions and for any electronic device structure. We show that the DG model reduces DD model error from as much as 50% down to a few percent in comparison to thin oxide MOS capacitance measurements. We also show the first DG simulations of gate oxide tunneling and transverse current flow in ultra-scaled MOSFETs. The advantages of rapid model implementation using the PDE solver approach will be demonstrated, as well as the applicability of the DG model to any electronic device structure.

Automatic Calculation of Hydrostatic Pressure Gradient in Patients with Head Injury: A Pilot Study.

PubMed

Moss, Laura; Shaw, Martin; Piper, Ian; Arvind, D K; Hawthorne, Christopher

2016-01-01

The non-surgical management of patients with traumatic brain injury is the treatment and prevention of secondary insults, such as low cerebral perfusion pressure (CPP). Most clinical pressure monitoring systems measure pressure relative to atmospheric pressure. If a patient is managed with their head tilted up, relative to their arterial pressure transducer, then a hydrostatic pressure gradient (HPG) can act against arterial pressure and cause significant errors in calculated CPP.To correct for HPG, the arterial pressure transducer should be placed level with the intracranial pressure transducer. However, this is not always achieved. In this chapter, we describe a pilot study investigating the application of speckled computing (or "specks") for the automatic monitoring of the patient's head tilt and subsequent automatic calculation of HPG. In future applications this will allow us to automatically correct CPP to take into account any HPG.

Temperature Dependence of Electrical Resistance of Woven Melt-Infiltrated SiCf/SiC Ceramic Matrix Composites

NASA Technical Reports Server (NTRS)

Appleby, Matthew P.; Morscher, Gregory N.; Zhu, Dongming

2016-01-01

Recent studies have successfully shown the use of electrical resistance (ER)measurements to monitor room temperature damage accumulation in SiC fiber reinforced SiC matrix composites (SiCf/SiC) Ceramic Matrix Composites (CMCs). In order to determine the feasibility of resistance monitoring at elevated temperatures, the present work investigates the temperature dependent electrical response of various MI (Melt Infiltrated)-CVI (Chemical Vapor Infiltrated) SiC/SiC composites containing Hi-Nicalon Type S, Tyranno ZMI and SA reinforcing fibers. Test were conducted using a commercially available isothermal testing apparatus as well as a novel, laser-based heating approach developed to more accurately simulate thermomechanical testing of CMCs. Secondly, a post-test inspection technique is demonstrated to show the effect of high-temperature exposure on electrical properties. Analysis was performed to determine the respective contribution of the fiber and matrix to the overall composite conductivity at elevated temperatures. It was concluded that because the silicon-rich matrix material dominates the electrical response at high temperature, ER monitoring would continue to be a feasible method for monitoring stress dependent matrix cracking of melt-infiltrated SiC/SiC composites under high temperature mechanical testing conditions. Finally, the effect of thermal gradients generated during localized heating of tensile coupons on overall electrical response of the composite is determined.

Thermoelectrically cooled temperature-gradient apparatus for comparative cell and virus temperature studies.

PubMed

Clark, H F; Kaminski, F; Karzon, D T

1970-05-01

Establishment of a near-linear temperature gradient in an incubator has been accomplished by the application of heat to one terminus of a conducting body, normally a metal bar, and the removal of heat from the other terminus of the conducting body. Such incubators have been complex and unwieldy because of the need for mechanical refrigeration. We have described a simplified temperature gradient incubator which uses thermoelectric module cooling coupled with electric heating. Along the gradient, 20 stations in two parallel rows of 10, each accommodating a 30-ml plastic cell culture flask, were continually monitored by an electronic thermometer, and the temperatures were recorded. By manipulation of two simple potentiometer controls, any temperature gradient between 0 and 50 C could be obtained. Minor deviations which occurred between theoretically perfect and obtained temperature gradients were reproducible and readily measured. The gradient incubator was particularly applicable to (i) simultaneously studying a given biological activity over the entire temperature range supporting the growth of a given cell, virus, or microorganism, or (ii) precisely defining the upper or lower temperature limits of a biological system by 10-point determinations. Preliminary experiments have demonstrated the usefulness of the apparatus in characterizing the temperature limits for growth in vitro of cells of reptilian cell lines. The gradient incubator was also successfully utilized for the characterization of the effect of temperature on the efficiency of plating of amphibian viruses and possible temperature variants of those viruses.

An in vitro hepatic zonation model with a continuous oxygen gradient in a microdevice.

PubMed

Sato, Asako; Kadokura, Kanae; Uchida, Hideyuki; Tsukada, Kosuke

2014-10-31

In a hepatic lobule, different sets of metabolic enzymes are expressed in the periportal (PP) and pericentral (PC) regions, forming a functional zonation, and the oxygen gradient is considered a determinant of zone formation. It is desirable to reproduce lobular microenvironment in vitro, but incubation of primary hepatocytes in conventional culture dishes has been limited at fixed oxygen concentrations due to technical difficulties. We designed a cell culture microdevice with an oxygen gradient to reproduce the hepatic microenvironment in vitro. The oxygen gradient during cell culture was monitored using a laser-assisted phosphorescence quenching method, and the cellular oxygen consumption rate could be estimated from changes in the gradient. Culture medium was continuously exchanged through microchannels installed in the device to maintain the oxygen gradient for a long term without transient hyper-oxygenation. The oxygen consumption rates of hepatocytes at 70.0mmHg and 31.4mmHg of partial oxygen pressure, which correspond to PP and PC regions in the microdevice, were 3.67×10(-10) and 3.15×10(-10)mol/s/10(6) cells, respectively. Antimycin A changed the oxygen gradient profile, indicating that cellular respiration can be estimated during cell culture. RT-PCR analysis of hepatocytes cultured under the oxygen gradient showed that mRNA expression of PEPCK and GK significantly increased in culture areas corresponding to PP and PC regions, respectively. These results indicate that the developed microdevice can reproduce the hepatic lobular microenvironment. The oxygen gradient in the microdevice can be closely controlled by changing the sizes of gas channels and the ambient oxygen concentration around the device; therefore, it could be expected to mimic the oxygen gradient of various organs, and it may be applicable to other pathological models. Copyright © 2014 Elsevier Inc. All rights reserved.

The use of biomarkers as integrative tools for transitional water bodies monitoring in the Water Framework Directive context - A holistic approach in Minho river transitional waters.

PubMed

Capela, R; Raimundo, J; Santos, M M; Caetano, M; Micaelo, C; Vale, C; Guimarães, L; Reis-Henriques, M A

2016-01-01

The Water Framework Directive (WFD) provides an important legislative opportunity to promote and implement an integrated approach for the protection of inland surface waters, transitional waters, coastal waters and groundwaters. The transitional waters constitute a central piece as they are usually under high environmental pressure and by their inherent characteristics present monitoring challenges. Integrating water quality monitoring with biological monitoring can increase the cost-effectiveness of monitoring efforts. One way of doing this is with biomarkers, which effectively integrate physical-chemical status and biological quality elements, dealing holistically with adverse consequences on the health of water bodies. The new Marine Strategy Framework Directive (MSFD) already incorporates the biomarker approach. Given the recent activities of OSPAR and HELCOM to harmonize existing monitoring guidelines between MSFD and WFD the use of similar methodologies should be fostered. To illustrate the potential of the biomarker approach, juveniles of flounder (Platichthys flesus) were used to evaluate the quality of the Minho river-estuary water bodies. The use of juveniles instead of adults eliminates several confounding factors such changes on the biological responses associated with reproduction. Here, a panel of well-established biomarkers, EROD, AChE, SOD, CAT, GST, LPO, ENA and FACs (1-Hydroxyrene) were selected and measured along with a gradient of different physical conditions, and integrated with trace elements characterization on both biota and sediments. In general, a clear profile along the water bodies was found, with low seasonal and spatial variation, consistent with a low impacted area. Overall, the results support the use of both the battery of biomarkers and the use of juvenile flounders in the monitoring of the water quality status within the WFD. Copyright © 2015 Elsevier B.V. All rights reserved.

Ground-water quality near a sewage-sludge recycling site and a landfill near Denver, Colorado

USGS Publications Warehouse

Robson, Stanley G.

1977-01-01

The Metropolitan Denver Sewage Disposal District and the City and County of Denver operate a sewage-sludge recycling site and a landfill in an area about 15 miles (24 kilometers) east of Denver. The assessment of the effects of these facilities on the ground-water system included determining the direction of ground-water movement in the area, evaluating the impact of the wastedisposal activities on the chemical quality of local ground water, and evaluating the need for continued water-quality monitoring.Surficial geology of the area consists of two principal units: (1) Alluvium with a maximum thickness of about 25 feet (7.6 meters) deposited along stream channels, and (2) bedrock consisting of undifferentiated Denver and Dawson Formations. Ground water in formations less than 350 feet (110 meters) deep moves to the north, as does surface flow, while ground water in formations between 570 and 1,500 feet (170 and 460 meters) deep moves to the west. Estimates of ground-water velocity were made using assumed values for hydraulic conductivity and porosity, and the observed hydraulic gradient from the study area. Lateral velocities are estimated to be 380 feet (120 meters) per year in alluvium and 27 feet (8.2 meters) per year in the upper part of the bedrock formations. Vertical velocity is estimated to be 0.58 foot (0.18 meter) per year in the upper part of the bedrock formations.Potentiometric head decreases with depth in the bedrock formations indicating a potential for downward movement of ground water. However, waterquality analysis and the rate and direction of ground-water movement suggest that ground-water movement in the area is primarily in the lateral rather than the vertical direction. Five wells perforated in alluvium were found to have markedly degraded water quality. One well was located in the landfill and water that was analyzed was obtained from near the base of the buried refuse, two others were located downgradient and near sewage-sludge burial areas, and the remaining two are located near stagnant surface ponds. Concentrations of nitrate in wells downgradient from fields where sludge is plowed into the soil were higher than background concentrations due to the effects of the sludge disposal. No evidence of water-quality degradation was detected in deeper wells perforated in the bedrock formations. Continued water-quality monitoring is needed because of the continuing disposal of wastes. A suggested monitoring program would consist of monitoring wells near the landfill twice a year and monitoring wells near the sludge-disposal areas on an annual basis.

Evaluation of multiple tracer methods to estimate low groundwater flow velocities

DOE PAGES

Reimus, Paul W.; Arnold, Bill W.

2017-02-20

Here, four different tracer methods were used to estimate groundwater flow velocity at a multiple-well site in the saturated alluvium south of Yucca Mountain, Nevada: (1) two single-well tracer tests with different rest or “shut-in” periods, (2) a cross-hole tracer test with an extended flow interruption, (3) a comparison of two tracer decay curves in an injection borehole with and without pumping of a downgradient well, and (4) a natural-gradient tracer test. Such tracer methods are potentially very useful for estimating groundwater velocities when hydraulic gradients are flat (and hence uncertain) and also when water level and hydraulic conductivity datamore » are sparse, both of which were the case at this test location. The purpose of the study was to evaluate the first three methods for their ability to provide reasonable estimates of relatively low groundwater flow velocities in such low-hydraulic-gradient environments. The natural-gradient method is generally considered to be the most robust and direct method, so it was used to provide a “ground truth” velocity estimate. However, this method usually requires several wells, so it is often not practical in systems with large depths to groundwater and correspondingly high well installation costs. The fact that a successful natural gradient test was conducted at the test location offered a unique opportunity to compare the flow velocity estimates obtained by the more easily deployed and lower risk methods with the ground-truth natural-gradient method. The groundwater flow velocity estimates from the four methods agreed very well with each other, suggesting that the first three methods all provided reasonably good estimates of groundwater flow velocity at the site. We discuss the advantages and disadvantages of the different methods, as well as some of the uncertainties associated with them.« less

Evaluation of multiple tracer methods to estimate low groundwater flow velocities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reimus, Paul W.; Arnold, Bill W.

Here, four different tracer methods were used to estimate groundwater flow velocity at a multiple-well site in the saturated alluvium south of Yucca Mountain, Nevada: (1) two single-well tracer tests with different rest or “shut-in” periods, (2) a cross-hole tracer test with an extended flow interruption, (3) a comparison of two tracer decay curves in an injection borehole with and without pumping of a downgradient well, and (4) a natural-gradient tracer test. Such tracer methods are potentially very useful for estimating groundwater velocities when hydraulic gradients are flat (and hence uncertain) and also when water level and hydraulic conductivity datamore » are sparse, both of which were the case at this test location. The purpose of the study was to evaluate the first three methods for their ability to provide reasonable estimates of relatively low groundwater flow velocities in such low-hydraulic-gradient environments. The natural-gradient method is generally considered to be the most robust and direct method, so it was used to provide a “ground truth” velocity estimate. However, this method usually requires several wells, so it is often not practical in systems with large depths to groundwater and correspondingly high well installation costs. The fact that a successful natural gradient test was conducted at the test location offered a unique opportunity to compare the flow velocity estimates obtained by the more easily deployed and lower risk methods with the ground-truth natural-gradient method. The groundwater flow velocity estimates from the four methods agreed very well with each other, suggesting that the first three methods all provided reasonably good estimates of groundwater flow velocity at the site. We discuss the advantages and disadvantages of the different methods, as well as some of the uncertainties associated with them.« less

The cost-effectiveness of hepatic venous pressure gradient monitoring in the prevention of recurrent variceal hemorrhage.

PubMed

Targownik, Laura E; Spiegel, Brennan M R; Dulai, Gareth S; Karsan, Hetal A; Gralnek, Ian M

2004-07-01

Recurrent variceal hemorrhage is common following an initial bleed in patients with cirrhosis. The current standard of care for secondary prophylaxis is endoscopic band ligation (EBL). Combination of beta-blocker and nitrate therapy, guided by hepatic venous pressure gradient (HVPG) monitoring, is a novel alternative strategy. We sought to determine the cost-effectiveness of these competing strategies. Decision analysis with Markov modeling was used to calculate the cost-effectiveness of three competing strategies: (1) EBL; (2) beta-blocker and nitrate therapy without HVPG monitoring (HVPG-); and (3) beta-blocker and nitrate therapy with HVPG monitoring (HVPG+). Patients in the HVPG+ strategy who failed to achieve an HVPG decline from medical therapy were offered EBL. Cost estimates were from a third-party payer perspective. The main outcome measure was the cost per recurrent variceal hemorrhage prevented. Under base-case conditions, the HVPG+ strategy was the most effective yet most expensive approach, followed by EBL and HVPG-. Compared to the EBL strategy, the HVPG+ strategy cost an incremental 5,974 dollars per recurrent bleed prevented. In a population with 100% compliance with all therapies, the incremental cost of HVPG-versus EBL fell to 5,270 dollars per recurrent bleed prevented. The model results were sensitive to the cost of EBL, the cost of HVPG monitoring, and the probability of medical therapy producing an adequate HVPG decline. Compared to EBL for the secondary prophylaxis of variceal rebleeding, combination medical therapy guided by HVPG monitoring is more effective and only marginally more expensive.

Gradients estimation from random points with volumetric tensor in turbulence

NASA Astrophysics Data System (ADS)

Watanabe, Tomoaki; Nagata, Koji

2017-12-01

We present an estimation method of fully-resolved/coarse-grained gradients from randomly distributed points in turbulence. The method is based on a linear approximation of spatial gradients expressed with the volumetric tensor, which is a 3 × 3 matrix determined by a geometric distribution of the points. The coarse grained gradient can be considered as a low pass filtered gradient, whose cutoff is estimated with the eigenvalues of the volumetric tensor. The present method, the volumetric tensor approximation, is tested for velocity and passive scalar gradients in incompressible planar jet and mixing layer. Comparison with a finite difference approximation on a Cartesian grid shows that the volumetric tensor approximation computes the coarse grained gradients fairly well at a moderate computational cost under various conditions of spatial distributions of points. We also show that imposing the solenoidal condition improves the accuracy of the present method for solenoidal vectors, such as a velocity vector in incompressible flows, especially when the number of the points is not large. The volumetric tensor approximation with 4 points poorly estimates the gradient because of anisotropic distribution of the points. Increasing the number of points from 4 significantly improves the accuracy. Although the coarse grained gradient changes with the cutoff length, the volumetric tensor approximation yields the coarse grained gradient whose magnitude is close to the one obtained by the finite difference. We also show that the velocity gradient estimated with the present method well captures the turbulence characteristics such as local flow topology, amplification of enstrophy and strain, and energy transfer across scales.

GOCE gravity gradient data for lithospheric modeling - From well surveyed to frontier areas

NASA Astrophysics Data System (ADS)

Bouman, J.; Ebbing, J.; Gradmann, S.; Fuchs, M.; Fattah, R. Abdul; Meekes, S.; Schmidt, M.; Lieb, V.; Haagmans, R.

2012-04-01

We explore how GOCE gravity gradient data can improve modeling of the Earth's lithosphere and thereby contribute to a better understanding of the Earth's dynamic processes. The idea is to invert satellite gravity gradients and terrestrial gravity data in the well explored and understood North-East Atlantic Margin and to compare the results of this inversion, providing improved information about the lithosphere and upper mantle, with results obtained by means of models based upon other sources like seismics and magnetic field information. Transfer of the obtained knowledge to the less explored Rub' al Khali desert is foreseen. We present a case study for the North-East Atlantic margin, where we analyze the use of satellite gravity gradients by comparison with a well-constrained 3D density model that provides a detailed picture from the upper mantle to the top basement (base of sediments). The latter horizon is well resolved from gravity and especially magnetic data, whereas sedimentary layers are mainly constrained from seismic studies, but do in general not show a prominent effect in the gravity and magnetic field. We analyze how gravity gradients can increase confidence in the modeled structures by calculating a sensitivity matrix for the existing 3D model. This sensitivity matrix describes the relation between calculated gravity gradient data and geological structures with respect to their depth, extent and relative density contrast. As the sensitivity of the modeled bodies varies for different tensor components, we can use this matrix for a weighted inversion of gradient data to optimize the model. This sensitivity analysis will be used as input to study the Rub' al Khali desert in Saudi Arabia. In terms of modeling and data availability this is a frontier area. Here gravity gradient data will be used to better identify the extent of anomalous structures within the basin, with the goal to improve the modeling for hydrocarbon exploration purposes.

Monitoring concentration and isotopic composition of methane in groundwater in the Utica Shale hydraulic fracturing region of Ohio.

PubMed

Claire Botner, E; Townsend-Small, Amy; Nash, David B; Xu, Xiaomei; Schimmelmann, Arndt; Miller, Joshua H

2018-05-03

Degradation of groundwater quality is a primary public concern in rural hydraulic fracturing areas. Previous studies have shown that natural gas methane (CH 4 ) is present in groundwater near shale gas wells in the Marcellus Shale of Pennsylvania, but did not have pre-drilling baseline measurements. Here, we present the results of a free public water testing program in the Utica Shale of Ohio, where we measured CH 4 concentration, CH 4 stable isotopic composition, and pH and conductivity along temporal and spatial gradients of hydraulic fracturing activity. Dissolved CH 4 ranged from 0.2 μg/L to 25 mg/L, and stable isotopic measurements indicated a predominantly biogenic carbonate reduction CH 4 source. Radiocarbon dating of CH 4 in combination with stable isotopic analysis of CH 4 in three samples indicated that fossil C substrates are the source of CH 4 in groundwater, with one 14 C date indicative of modern biogenic carbonate reduction. We found no relationship between CH 4 concentration or source in groundwater and proximity to active gas well sites. No significant changes in CH 4 concentration, CH 4 isotopic composition, pH, or conductivity in water wells were observed during the study period. These data indicate that high levels of biogenic CH 4 can be present in groundwater wells independent of hydraulic fracturing activity and affirm the need for isotopic or other fingerprinting techniques for CH 4 source identification. Continued monitoring of private drinking water wells is critical to ensure that groundwater quality is not altered as hydraulic fracturing activity continues in the region. Graphical abstract A shale gas well in rural Appalachian Ohio. Photo credit: Claire Botner.

Remote-loading labeling of liposomes with (99m)Tc-BMEDA and its stability evaluation: effects of lipid formulation and pH/chemical gradient.

PubMed

Li, Shihong; Goins, Beth; Phillips, William T; Bao, Ande

2011-03-01

Efficient, convenient, and stable radiolabeling plays a critical role for the monitoring of liposome behavior via either blood sampling, organ distribution, or noninvasive nuclear imaging. The direct labeling of liposome-carrying drugs without any prior modification undoubtedly is convenient and optimal for liposomal drug testing. In this article, we investigated the effect of various lipid formulations and pH/chemical gradients on the radiolabeling efficiency and entrapment stability of technetium-99m ((99m)Tc) remotely loaded into liposomes, using (99m)Tc-N,N-bis(2-mercaptoethyl)-N',N'-diethyl-ethylenediamine ((99m)Tc-BMEDA) complex. The tested liposomes either contained unsaturated lipid or possessed various surface charges. (99m)Tc could be efficiently loaded into various premanufactured liposomes containing either an ammonium sulfate pH, citrate pH, or glutathione (GSH) chemical gradient. (99m)Tc-entrapment stabilities of these liposomes in phosphate-buffered saline (PBS; pH 7.4) buffer at 25°C were mainly dependent on the pH/chemical gradient, but not lipid formulation. Stability sequence was ammonium sulfate pH-gradient>citrate pH-gradient>GSH-gradient. Stabilities of (99m)Tc-liposomes in 50% fetal bovine serum (FBS)/PBS (pH 7.4) buffer at 37°C are dependent on both lipid formulation and pH/chemical gradient. Specifically, (99m)Tc labeling of the ammonium sulfate pH-gradient liposomes were less stable in 50% FBS/PBS than in PBS, whereas noncationic liposomes with citrate pH- or GSH-gradient displayed higher stability, except that anionic citrate pH-gradient liposomes showed no stability difference in these two media. Cationic liposomes aggregated in 50% FBS/PBS, forming a new discrete fraction with larger particle sizes. These in vitro characterization results have indicated the optimism of using (99m)Tc-BMEDA for labeling pH/GSH gradient liposomes without the requirement of modifying lipid formulation for liposomal therapeutic-agent development.

A MAIL SURVEY APPROACH TO WATERSHED CONDITION ASSESSMENT

EPA Science Inventory

Aquatic resource monitoring attempts to assess the condition of aquatic habitat and organisms. Assessments require that disturbances from human activities be identified, quantified and ordered along a gradient for interpreting biological response. An index of relative risk to a...

The technological future of 7 T MRI hardware.

PubMed

Webb, A G; Van de Moortele, P F

2016-09-01

In this article we present our projections of future hardware developments on 7 T human MRI systems. These include compact cryogen-light magnets, improved gradient performance, integrated RF-receive and direct current shimming coil arrays, new RF technology with adaptive impedance matching, patient-specific specific absorption rate estimation and monitoring, and increased integration of physiological monitoring systems. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

Geothermal Gradient impact on Induced Seismicity in Raton Basin, Colorado and New Mexico

NASA Astrophysics Data System (ADS)

Pfeiffer, K.; Ge, S.

2017-12-01

Since 1999, Raton Basin, located in southeastern Colorado and northern New Mexico, is the site of wastewater injection for disposing a byproduct of coal bed methane production. During 1999-2016, 29 wastewater injection wells were active in Raton Basin. Induced seismicity began in 2001 and the largest recorded earthquake, an M5.3, occurred in August 2011. Although most injection occurs in the Dakota Formation, the majority of the seismicity has been located in the crystalline basement. Previous studies involving Raton Basin focused on high injection rates and high volume wells to determine their effect on increased pore pressure. However, the geothermal gradient has yet to be studied as a potential catalyst of seismicity. Enhanced Geothermal Systems throughout the world have experienced similar seismicity problems due to water injection. Raton's geothermal gradient, which averages 49± 12°C/km, is much higher then other areas experiencing seismicity. Thermal differences between the hot subsurface and cooler wastewater injection have the potential to affect the strength of the rock and allow for failure. Therefore, we hypothesis that wells in high geothermal gradient areas will produce more frequent earthquakes due to thermal contrast from relatively cold wastewater injection. We model the geothermal gradient in the surrounding areas of the injection sites in Raton Basin to assess potential spatial relationship between high geothermal gradient and earthquakes. Preliminary results show that the fluid pressure increase from injecting cool water is above the threshold of 0.1MPa, which has been shown to induce earthquakes. In addition, temperatures in the subsurface could decrease up to 2°C at approximately 80 m from the injection well, with a temperature effect reaching up to 100 m away from the injection well.

An adaptive morphological gradient lifting wavelet for detecting bearing defects

NASA Astrophysics Data System (ADS)

Li, Bing; Zhang, Pei-lin; Mi, Shuang-shan; Hu, Ren-xi; Liu, Dong-sheng

2012-05-01

This paper presents a novel wavelet decomposition scheme, named adaptive morphological gradient lifting wavelet (AMGLW), for detecting bearing defects. The adaptability of the AMGLW consists in that the scheme can select between two filters, mean the average filter and morphological gradient filter, to update the approximation signal based on the local gradient of the analyzed signal. Both a simulated signal and vibration signals acquired from bearing are employed to evaluate and compare the proposed AMGLW scheme with the traditional linear wavelet transform (LWT) and another adaptive lifting wavelet (ALW) developed in literature. Experimental results reveal that the AMGLW outperforms the LW and ALW obviously for detecting bearing defects. The impulsive components can be enhanced and the noise can be depressed simultaneously by the presented AMGLW scheme. Thus the fault characteristic frequencies of bearing can be clearly identified. Furthermore, the AMGLW gets an advantage over LW in computation efficiency. It is quite suitable for online condition monitoring of bearings and other rotating machineries.

Gradient optimization and nonlinear control

NASA Technical Reports Server (NTRS)

Hasdorff, L.

1976-01-01

The book represents an introduction to computation in control by an iterative, gradient, numerical method, where linearity is not assumed. The general language and approach used are those of elementary functional analysis. The particular gradient method that is emphasized and used is conjugate gradient descent, a well known method exhibiting quadratic convergence while requiring very little more computation than simple steepest descent. Constraints are not dealt with directly, but rather the approach is to introduce them as penalty terms in the criterion. General conjugate gradient descent methods are developed and applied to problems in control.

Experiments on microjets of undercooled liquid hydrogen

NASA Astrophysics Data System (ADS)

Fernández, José M.; Kühnel, Matthias; Tejeda, Guzmán; Kalinin, Anton; Grisenti, Robert E.; Montero, Salvador

2012-11-01

Novel experiments on liquid microjets (filaments) of hydrogen and deuterium, carried out at the Laboratory of Molecular Fluid Dynamics of the IEM, are reported. These filaments, less than 10 microns in diameter, are an ideal medium to produce highly undercooled liquid samples and to investigate the homogeneous solidification process, free from wall effects. The filaments exit from cryogenic capillary nozzles into a vacuum chamber, to cool down very fast by surface evaporation. Finite size radius leads to a temperature gradient across the filament, determined by thermal conductivity, and, possibly, to a velocity gradient as well. The filaments are monitored by laser shadowgraphy, and analyzed by means of high performance Raman spectroscopy. Real-time measurements in the rotational and vibrational spectral regions reveal the structure and temperature along the filaments, allowing to track the crystal growth process. The high spatial resolution of Raman spectroscopy allows observing in situ the structural changes of the liquid microjets, with a time resolution of ˜ 10 ns. The filaments of pure para-H2 can be cooled down to 9 K (65% of its melting point at 13.8 K), while staying liquid, before eventually solidifying into a metastable polymorph. Crystallization kinetics revealed a growth rate of 33 cm/s, much higher than expected for a thermally activated process. The time and spatial control attained in these experiments offers new opportunities for investigating the processes of nonequilibrium phase transformations in undercooled fluids, as well as the propagation of liquid jets into a rarefied gas media.

Potential aquifer vulnerability in regions down-gradient from uranium in situ recovery (ISR) sites.

PubMed

Saunders, James A; Pivetz, Bruce E; Voorhies, Nathan; Wilkin, Richard T

2016-12-01

Sandstone-hosted roll-front uranium ore deposits originate when U(VI) dissolved in groundwater is reduced and precipitated as insoluble U(IV) minerals. Groundwater redox geochemistry, aqueous complexation, and solute migration are important in leaching uranium from source rocks and transporting it in low concentrations to a chemical redox interface where it is deposited in an ore zone typically containing the uranium minerals uraninite, pitchblende, and/or coffinite; various iron sulfides; native selenium; clays; and calcite. In situ recovery (ISR) of uranium ores is a process of contacting the uranium mineral deposit with leaching and oxidizing (lixiviant) fluids via injection of the lixiviant into wells drilled into the subsurface aquifer that hosts uranium ore, while other extraction wells pump the dissolved uranium after dissolution of the uranium minerals. Environmental concerns during and after ISR include water quality degradation from: 1) potential excursions of leaching solutions away from the injection zone into down-gradient, underlying, or overlying aquifers; 2) potential migration of uranium and its decay products (e.g., Ra, Rn, Pb); and, 3) potential mobilization and migration of redox-sensitive trace metals (e.g., Fe, Mn, Mo, Se, V), metalloids (e.g., As), and anions (e.g., sulfate). This review describes the geochemical processes that control roll-front uranium transport and fate in groundwater systems, identifies potential aquifer vulnerabilities to ISR operations, identifies data gaps in mitigating these vulnerabilities, and discusses the hydrogeological characterization involved in developing a monitoring program. Published by Elsevier Ltd.

Intercomparison of Groundwater Flow Monitoring Technologies at Site OU 1, Former Fort Ord, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

Daley, P F; Jantos, J; Pedler, W H

2005-09-20

This report presents an intercomparison of three groundwater flow monitoring technologies at a trichloroethylene (TCE) groundwater plume at Operational Unit 1 (OU 1) adjacent to the former Fritzsche Army Airfield at the former Fort Ord Army Base, located on Monterey Bay in northern Monterey County, California. Soil and groundwater at this site became contaminated by fuels and solvents that were burned on a portion of OU 1 called the Fire Drill Area (FDA) as part of firefighter training from 1962 and 1985. Cont Contamination is believed to be restricted to the unconfined A-aquifer, where water is reached at a depthmore » of approximately 60 to 80 feet below the ground surface; the aquifer is from 15 to 20 feet in thickness, and is bounded below by a dense clay layer, the Salinas Valley Aquitard. Soil excavation and bioremediation were initiated at the site of fire training activities in the late 1980s. Since that time a pump-and-treat operation has been operated close to the original area of contamination, and this system has been largely successful at reducing groundwater contamination in this source area. However, a trichloroethylene (TCE) groundwater plume extends approximately 3000 ft (900 m) to the northwest away from the FDA. In this report, we have augmented flow monitoring equipment permanently installed in an earlier project (Oldenburg et al., 2002) with two additional flow monitoring devices that could be deployed in existing monitoring wells, in an effort to better understand their performance in a nearly ideal, homogeneous sand aquifer, that we expected would exhibit laminar groundwater flow owing to the site's relatively simple hydrogeology. The three flow monitoring tools were the Hydrotechnics{reg_sign} In In-Situ Permeable Flow Sensor (ISPFS), the RAS Integrated Subsurface Evaluation Hydrophysical Logging tool (HPL), and the Lawrence Livermore National Laboratory Scanning Colloidal Borescope Flow Meter (SCBFM). All three devices produce groundwater flow velocity measurements, and the ISPFS and SCBFM systems also gene generate flow direction rate estimates. The ISPFS probes are permanently installed and are non-retrievable, but produce long-term records with essentially no operator intervention or maintenance. The HPL and SCBFM systems are lightweight, portable logging devices that employ recording of electrical conductivity changes in wells purged with deionized water (HPL), or imaging of colloidal particles traversing the borehole (SCBFM) as the physical basis for estimating the velocity of groundwater flow through monitoring wells. All three devices gave estimates of groundwater velocity that were in reasonable agreement. However, although the ISPFS produced groundwater azimuth data that correlated well with conventional conductivity and gradient analyses of the groundwater flow field, the SCBFM direction data were in poor agreement. Further research into the reasons for this lack of correlation would seem to be warranted, given the ease of deployment of this tool in existing conventional monitoring wells, and its good agreement with the velocity estimates of the other technologies examined.« less

Conductivity detection for monitoring mixing reactions in microfluidic devices.

PubMed

Liu, Y; Wipf, D O; Henry, C S

2001-08-01

A conductivity detector was coupled to poly(dimethylsiloxane)-glass capillary electrophoresis microchips to monitor microfluidic flow. Electroosmotic flow was investigated with both conductivity detection (CD) and the current monitoring method. No significant variation was observed between these methods, but CD showed a lower relative standard deviation. Gradient mixing experiments were employed to investigate the relationship between the electrolyte conductivity and the electrolyte concentration. A good linear response of conductivity to concentration was obtained for solutions whose difference in concentrations were less than 27 mM. The new system holds great promise for precision mixing in microfluidic devices using electrically driven flows.

Separation and quantitative determination of 6α-hydroxycortisol and 6β-hydroxycortisol in human urine by high-performance liquid chromatography with ultraviolet absorption detection.

PubMed

Shibasaki, Hiromi; Okamoto, Sawako; Inoue, Risako; Okita, Misato; Yokokawa, Akitomo; Furuta, Takashi

2012-03-01

The present study developed an high-performance liquid chromatography (HPLC) method for the simultaneous determination of urinary metabolites of endogenous cortisol, 6α-hydroxycortisol (6α-OHF) and 6β-hydroxycortisol (6β-OHF), in human urine, using 6α-hydroxycorticosterone as internal standard. 6α-OHF and 6β-OHF were extracted from urine with ethyl acetate by using a Sep-Pak C(18) plus cartridge. Separation of the stereoisomers was achieved on a reversed-phase hybrid column by a gradient elution of (A) 0.05 M KH(2)PO(4)-0.01 M CH(3)COOH (pH 3.77) and (B) 0.05 M KH(2)PO(4)-0.01 M CH(3)COOH/acetonitrile (2:3, v/v). 6α-OHF and 6β-OHF were well separated on an XTerra MS C(18) 5 μm column using two types of stepwise gradient elution program (programs 2 and 3). Resolutions of 6α-OHF and 6β-OHF were Rs = 4.41 for program 2 and Rs = 4.60 for program 3. The analysis was performed within 23~26 min, monitored by UV absorbance at 239 nm. The lower limits of detection of 6α-OHF and 6β-OHF were 0.80 ng per injection (s/n = ca. 8), and the lower limits of quantification were 5.02 ng/ml for 6α-OHF and 41.08 ng/ml for 6β-OHF, respectively. The within-day reproducibilities in the amounts of 6α-OHF and 6β-OHF determined were in good agreement with the actual amounts added, the relative errors being -5.37% and -3.73% (gradient 2) and -5.69% and -3.96% (gradient 3) for both 6α-OHF and 6β-OHF, respectively. The inter-assay precisions (RSDs) for 6α-OHF and 6β-OHF were less than 1.99% (gradient 2) and 2.61% (gradient 3), respectively. The present HPLC method was applied to the measurement of 6α-OHF and 6β-OHF in urine to evaluate the time courses of 6α-hydroxylation and 6β-hydroxylation clearances of cortisol during 40 days for phenotyping CYP3A in a healthy subject.

Quantifying structural physical habitat attributes using LIDAR and hyperspectral imagery - PRK

EPA Science Inventory

Structural physical habitat attributes include indices of stream size, channel gradient, substrate size, habitat complexity, and riparian vegetation cover and structure. The Environmental Monitoring and Assessment Program (EMAP) is designed to assess the status and trends of ecol...

ANALYSIS OF MACROINVERTEBRATE ASSEMBLAGES IN RELATION TO ENVIRONMENTAL GRADIENTS IN ROCKY MOUNTAIN STREAMS

EPA Science Inventory

Using redundancy analysis (RDA) and canonical correspondence analysis (CCA), we assessed relationships among chemical and physical characteristics and macroinvertebrate assemblages at stream sites sampled by the Regional Environmental Monitoring and Assessment Program (R-EMAP) in...

Quantifying Structural Physical Habitat Attributes Using Lidar and Hyperspectral Imagery (1)

EPA Science Inventory

Structural physical habitat attributes include indices of stream size, channel gradient, substrate size, habitat complexity, and riparian vegetation cover and structure. The Environmental Monitoring and Assessment Program (EMAP) is designed to assess the status and trends of eco...

Riparian hydraulic gradient and stream-groundwater exchange dynamics in steep headwater valleys

Treesearch

T.J. Voltz; M.N. Gooseff; A.S. Ward; K. Singha; M. Fitzgerald; T. Wagener

2013-01-01

Patterns of riparian hydraulic gradients and stream-groundwater exchange in headwater catchments provide the hydrologic context for important ecological processes. Although the controls are relatively well understood, their dynamics during periods of hydrologic change is not. We investigate riparian hydraulic gradients over three different time scales in two steep,...

Microfluidic liquid-air dual-gradient chip for synergic effect bio-evaluation of air pollutant.

PubMed

Liu, Xian-Jun; Hu, Shan-Wen; Xu, Bi-Yi; Zhao, Ge; Li, Xiang; Xie, Fu-Wei; Xu, Jing-Juan; Chen, Hong-Yuan

2018-05-15

In this paper, a novel prototype liquid-air dual gradient chip is introduced, which has paved the way for effective synergic effect bio-evaluation of air pollutant. The chip is composed of an array of the agarose liquid-air interfaces, top air gradient layer and bottom liquid gradient layer. The novel agarose liquid-air interface allows for non-biased exposure of cells to all the substances in the air and diffusive interactions with the liquid phase; while the dual liquid-air gradient provides powerful screening abilities, which well reduced errors, saved time and cost from repeated experiment. Coupling the two functions, the chip subsequently facilitates synergic effect evaluation of both liquid and air factors on cells. Here cigarette smoke was taken as the model air pollutant, and its strong synergic effects with inflammatory level of A549 lung cancer cells on their fate were successfully quantified for the first time. These results well testified that the proposed dual-gradient chip is powerful and indispensable for bio-evaluation of air pollutant. Copyright © 2018 Elsevier B.V. All rights reserved.

Physiological effects of pH gradients on Escherichia coli during plasmid DNA production.

PubMed

Cortés, José T; Flores, Noemí; Bolívar, Francisco; Lara, Alvaro R; Ramírez, Octavio T

2016-03-01

A two-compartment scale-down system was used to mimic pH heterogeneities that can occur in large-scale bioreactors. The system consisted of two interconnected stirred tank reactors (STRs) where one of them represented the conditions of the bulk of the fluid and the second one the zone of alkali addition for pH control. The working volumes ratio of the STRs was set to 20:1 in order to simulate the relative sizes of the bulk and alkali addition zones, respectively, in large-scale bioreactors. Residence times (tR ) in the alkali addition STR of 60, 120, 180, and 240 s were simulated during batch cultures of an engineered Escherichia coli strain that produced plasmid DNA (pDNA). pH gradients of up to 0.9 units, between the two compartments, were attained. The kinetic, stoichiometric, and pDNA topological changes due to the pH gradients were studied and compared to cultures at constant pH of 7.2 and 8.0. As the tR increased, the pDNA and biomass yields, as well as pDNA final titer decreased, whereas the accumulation of organic acids increased. Furthermore, the transcriptional response of 10 selected genes to alkaline stress (pH 8.0) and pH gradients was monitored at different stages of the cultures. The selected genes coded for ion transporters, amino acids catabolism enzymes, and transcriptional regulators. The transcriptional response of genes coding for amino acids catabolism, in terms of relative transcription level and stage of maximal expression, was different when the alkaline stress was constant or transient. This suggests the activation of different mechanisms by E. coli to cope with pH fluctuations compared to constant alkaline pH. Moreover, the transcriptional response of genes related to negative control of DNA synthesis did not correlate with the lower pDNA yields. This is the first study that reports the effects of pH gradients on pDNA production by E. coli cultures. The information presented can be useful for the design of better bioreactor scale-up strategies. © 2015 Wiley Periodicals, Inc.

Evaluating the effectiveness of the Minamata Convention on Mercury: Principles and recommendations for next steps.

PubMed

Evers, David C; Keane, Susan Egan; Basu, Niladri; Buck, David

2016-11-01

The Minamata Convention on Mercury is a multilateral environmental agreement that obligates Parties to reduce or control sources of mercury pollution in order to protect human health and the environment. The Convention includes provisions on providing technical assistance and capacity building, particularly for developing countries and countries with economies in transition, to promote its effective implementation. Evaluating the effectiveness of the Convention (as required by Article 22) is a crucial component to ensure that it meets this objective. We describe an approach to measure effectiveness, which includes a suite of short-, medium-, and long-term metrics related to five major mercury control Articles in the Convention, as well as metrics derived from monitoring of mercury in the environment using select bioindicators, including people. The use of existing biotic Hg data will define spatial gradients (e.g., biological mercury hotspots), baselines to develop relevant temporal trends, and an ability to assess risk to taxa and human communities of greatest concern. We also recommend the development of a technical document that describes monitoring options for the Conference of Parties, to provide science-based standardized guidelines for collecting relevant monitoring information, as guided by Article 19. Copyright © 2016 Elsevier B.V. All rights reserved.

Health monitoring of carbon cantilever using femtosecond laser inscribed FBG array in gradient-index CYTOP polymer fibre

NASA Astrophysics Data System (ADS)

Theodosiou, Antreas; Kalli, Kyriacos; Komodromos, Michael

2017-04-01

We report on the femtosecond laser inscription of a fibre Bragg grating array in multimode, gradient-index, CYTOP polymer optical fibre and its demonstration as a quasi-distributed sensor for cantilever health monitoring measurements. We exploit the key advantage of polymer optical fibres, having a significantly lower Young's modulus compared with silica fibres, for vibration measurements. We also modify the typical multi-mode Bragg grating spectrum through control of the femtosecond laser inscription process, thereby producing gratings having single peak wavelength spectra. The sensor array is used to recover the time-dependent, wavelength response from each Bragg grating sensor and extract the mode shape of the beam. The mode shapes of the beam were used to observe "damage" introduced to the cantilever by adding masses to its surface; adjusting the level of damage by using different weights and placing them at different point across the beam. We show that health monitoring measurements are feasible with polymer based fibre Bragg gratings. The accurate and rapid detection of damage points on structural beams and the damage level is an important parameter for improved maintenance and servicing of beams under load and for the prevention of long-term damage.

Have bird distributions shifted along an elevational gradient on a tropical mountain?

PubMed

Campos-Cerqueira, Marconi; Arendt, Wayne J; Wunderle, Joseph M; Aide, T Mitchell

2017-12-01

An upward shift in elevation is one of the most conspicuous species responses to climate change. Nevertheless, downward shifts and, apparently, the absences of response have also been recently reported. Given the growing evidence of multiple responses of species distributions due to climate change and the paucity of studies in the tropics, we evaluated the response of a montane bird community to climate change, without the confounding effects of land-use change. To test for elevational shifts, we compared the distribution of 21 avian species in 1998 and 2015 using occupancy models. The historical data set was based on point counts, whereas the contemporary data set was based on acoustic monitoring. We detected a similar number of species in historical (36) and contemporary data sets (33). We show an overall pattern of no significant change in range limits for most species, although there was a significant shift in the range limit of eight species (38%). Elevation limits shifted mostly upward, and this pattern was more common for upper than lower limits. Our results highlight the variability of species responses to climate change and illustrate how acoustic monitoring provides an easy and powerful way to monitor animal populations along elevational gradients.

[Changes in PCO2 and pH gradients in a canine model of experimental acute hemorrhage].

PubMed

Rabanal, J M; Mons, R; Galindo, M; Solares, G; Seco, I; García-Castrillo, L

1999-01-01

To study changes in arterial-venous PCO2 pressures (Da-vPCO2) and in pH (Da-vpH) in an experimental dog model of acute bleeding. To emphasize the utility of monitoring these variables in situations of acute bleeding. ANIMALS AND METHOD: Eighteen dogs were anesthesized and monitored while being subjected to gradual bleeding. Gasometric and hemodynamic variables were recorded at the following moments: baseline, stage I (15% of volemic loss), stage Ib (20%), stage II (25%), stage IIb (30%) and stage III (35%). No significant differences in pH or PCO2 in either arterial or mixed venous blood were found. Bleeding induced a constant and progressive increase in Da-vPCO2 that correlated with changes in volume (r = -0.56, p < 0.001), SvO2 (r = 0.71; p < 0.001), Da-vO2 gradient (r = 0.62; p < 0.001) and cardiac output (r = 0.63; p < 0.001). Gradual bleeding caused a constant and significant increase of Da-vPCO2. Monitoring such changes in states of circulatory insufficiency may be useful for evaluating tissue ischemia.

Interpretation of geophysical logs, aquifer tests, and water levels in wells in and near the North Penn Area 7 Superfund site, Upper Gwynedd Township, Montgomery County, Pennsylvania, 2000-02

USGS Publications Warehouse

Senior, Lisa A.; Cinotto, Peter J.; Conger, Randall W.; Bird, Philip H.; Pracht, Karl A.

2005-01-01

Ground water in the vicinity of various industrial facilities in Upper Gwynedd Township and Lansdale Borough, Montgomery County, Pa., is contaminated with various volatile organic compounds (VOCs). The 2-square-mile area was placed on the National Priorities List as the North Penn Area 7 Superfund site by the U.S. Environmental Protection Agency (USEPA) in 1989. The U.S. Geological Survey (USGS) conducted geophysical logging, aquifer testing, water-level monitoring, and streamflow measurements in the vicinity of North Penn Area 7 beginning autumn 2000 to assist the USEPA in developing an understanding of the hydrogeologic framework in the area as part of the USEPA Remedial Investigation. The study area is underlain by Triassic and Jurassic-age sandstones, siltstones, and shales of the Lockatong Formation and the Brunswick Group. Regionally, these rocks strike northeast and dip to the northwest. The sequence of rocks form a fractured-sedimentary-rock aquifer that acts as a set of confined to partially confined layered aquifers of differing permeabilities. The aquifers are recharged by precipitation and discharge to streams and wells. The Wissahickon Creek headwaters are less than 1 mile northeast of the study area, and this stream flows southwest to bisect North Penn Area 7. Ground water is pumped in the vicinity of North Penn Area 7 for industrial use and public supply. The USGS collected geophysical logs for 16 wells that ranged in depth from 50 to 623 feet. Aquifer-interval-isolation testing was done in 9 of the 16 wells, for a total of 30 zones tested. A multiple-well aquifer test was conducted by monitoring the response of 14 wells to pumping a 600-ft deep production well in February and March 2002. In addition, water levels were monitored continuously in three wells in the area and streamflow was measured quarterly at two sites on Wissahickon Creek from December 2000 through September 2002. Geophysical logging identified water-bearing zones associated with high-angle fractures and bedding-plane openings throughout the depth of the boreholes. Heatpulse-flowmeter measurements under nonpumping, ambient conditions indicated that borehole flow, where detected, was in the upward direction in three of the eight wells and in the downward direction in three wells. In two wells, both upward and downward flow were measured. Heatpulse-flowmeter measurements under pumping conditions were used to identify the most productive intervals in wells. Correlation of natural-gamma-ray and single-point-resistance logs indicated that bedding in the area probably strikes about 40 degrees northeast and dips from 6 to 7 degrees northwest. Aquifer intervals isolated by inflatable packers in wells were pumped to test productivity and to collect samples to determine chemical quality of water produced from the interval. Interval-isolation testing confirmed the presence of vertical hydraulic gradients indicated by heatpulse-flowmeter measurements. The specific capacities of isolated intervals ranged over two orders of magnitude, from 0.02 to more than 3.6 gallons per minute per foot. Intervals adjacent to isolated pumped intervals showed little response to pumping the isolated zone. The presence of vertical hydraulic gradients and lack of adjacent-interval response to pumping in isolated intervals indicate a limited degree of vertical hydraulic connection between the aquifer intervals tested. Concentrations of most VOC contaminants generally were highest in well-water samples from the shallowest isolated intervals, with some exceptions. Trichloroethylene, cis-1,2-dichloroethylene, and toluene were the most frequently detected VOCs, with maximum concentrations of greater than 340, 680, and greater than 590 micrograms per liter, respectively. Results of the aquifer test with multiple observation wells showed that water levels in 4 of the 14 wells declined in response to pumping. The four wells that responded to pumping are either along str

[PM₂.₅ Background Concentration at Different Directions in Beijing in 2013].

PubMed

Li, Yun-ting; Cheng, Niam-liang; Zhang, Da-wei; Sun, Rui-wen; Dong, Xin; Sun, Nai-di; Chen, Chen

2015-12-01

PM₂.₅, background concentration at different directions in 2013 in Beijing was analyzed combining the techniques of mathematical statistics, physical identification and numerical simulation (CMAQ4.7.1) as well as using monitoring data of six PM₂.₅ auto-monitoring sites and five meteorological sites in 2013. Results showed that background concentrations of PM₂.₅ at northwest, northeast, eastern, southeast, southern and southwest boundary sites were between 40.3 and 85.3 µg · m⁻³ in Beijing. From the lowest to the highest, PMPM₂.₅ background concentrations at different sites were: Miyun reservoir, Badaling, Donggaocun, Yufa, Yongledian and Liulihe. Background concentration of PM₂.₅ was the lowest under north wind, then under west wind, and significantly higher under south and east wind. Calculated PM₂.₅ background average concentrations were 6.5-27.9, 22.4-73.4, 67.2-91.7, 40.7-116.1 µg · m⁻³ respectively in different wind directions. Simulated PM₂.₅ background concentration showed a clear north-south gradient distribution and the surrounding area had a notable effect on the spatial distribution of PM₂.₅ background concentration in 2013 in Beijing.

Real-time oil-saturation monitoring in rock cores with low-field NMR.

PubMed

Mitchell, J; Howe, A M; Clarke, A

2015-07-01

Nuclear magnetic resonance (NMR) provides a powerful suite of tools for studying oil in reservoir core plugs at the laboratory scale. Low-field magnets are preferred for well-log calibration and to minimize magnetic-susceptibility-induced internal gradients in the porous medium. We demonstrate that careful data processing, combined with prior knowledge of the sample properties, enables real-time acquisition and interpretation of saturation state (relative amount of oil and water in the pores of a rock). Robust discrimination of oil and brine is achieved with diffusion weighting. We use this real-time analysis to monitor the forced displacement of oil from porous materials (sintered glass beads and sandstones) and to generate capillary desaturation curves. The real-time output enables in situ modification of the flood protocol and accurate control of the saturation state prior to the acquisition of standard NMR core analysis data, such as diffusion-relaxation correlations. Although applications to oil recovery and core analysis are demonstrated, the implementation highlights the general practicality of low-field NMR as an inline sensor for real-time industrial process control. Copyright © 2015 Elsevier Inc. All rights reserved.

Integrating social determinants of health in the universal health coverage monitoring framework.

PubMed

Vega, Jeanette; Frenz, Patricia

2013-12-01

Underpinning the global commitment to universal health coverage (UHC) is the fundamental role of health for well-being and sustainable development. UHC is proposed as an umbrella health goal in the post-2015 sustainable development agenda because it implies universal and equitable effective delivery of comprehensive health services by a strong health system, aligned with multiple sectors around the shared goal of better health. In this paper, we argue that social determinants of health (SDH) are central to both the equitable pursuit of healthy lives and the provision of health services for all and, therefore, should be expressly incorporated into the framework for monitoring UHC. This can be done by: (a) disaggregating UHC indicators by different measures of socioeconomic position to reflect the social gradient and the complexity of social stratification; and (b) connecting health indicators, both outcomes and coverage, with SDH and policies within and outside of the health sector. Not locating UHC in the context of action on SDH increases the risk of going down a narrow route that limits the right to health to coverage of services and financial protection.

Characterization of nucleosides and nucleobases in natural Cordyceps by HILIC-ESI/TOF/MS and HILIC-ESI/MS.

PubMed

Zhao, Heng-Qiang; Wang, Xiao; Li, Hong-Mei; Yang, Bin; Yang, Hong-Jun; Huang, Luqi

2013-08-15

A method combining hydrophilic interaction chromatography (HILIC) and electrospray ionization mass spectrometry (ESI-MS) was developed for the characterization and determination of natural Cordyceps. Separation was achieved on a Waters Xbridge Amide column with gradient elution. Identification of 15 target nucleosides and nucleobases was based on retention time, UV spectra and mass measurements of the protonated molecules ([M+H]⁺) and main fragment ions (ESI-TOF/MS). Eight non-target compounds were tentatively identified by ESI-TOF/MS. The 15 target compounds were quantified by HILIC-ESI-MS/MS using time-programmed selective ion monitoring or multiple reaction monitoring in positive-ion mode under optimized mass conditions. This technique showed good linearity, repeatability and recovery. This approach was also successfully implemented in the analysis of nucleosides and nucleobases in 12 batches of natural Cordyceps samples that were collected from different regions in China. The developed HILIC-ESI-MS method exhibited clear advantages in identifying and determining highly polar bioactive components in Cordyceps, as well as their quality control.

Damage Accumulation and Failure of Plasma-Sprayed Thermal Barrier Coatings under Thermal Gradient Cyclic Conditions

NASA Technical Reports Server (NTRS)

Zhu, Dongming; Choi, Sung R.; Ghosn, Louis J.; Miller, rober A.

2005-01-01

Thermal barrier coatings will be more aggressively designed to protect gas turbine engine hot-section components in order to meet future engine higher fuel efficiency and lower emission goals. A fundamental understanding of the sintering and thermal cycling induced delamination of thermal barrier coating systems under engine-like heat flux conditions will potentially help to improve the coating temperature capability. In this study, a test approach is established to emphasize the real-time monitoring and assessment of the coating thermal conductivity, which can initially increase under the steady-state high temperature thermal gradient test due to coating sintering, and later decrease under the thermal gradient cyclic test due to coating cracking and delamination. Thermal conductivity prediction models have been established for a ZrO2-(7- 8wt%)Y2O3 model coating system in terms of heat flux, time, and testing temperatures. The coating delamination accumulation is then assessed based on the observed thermal conductivity response under the combined steady-state and cyclic thermal gradient tests. The coating thermal gradient cycling associated delaminations and failure mechanisms under simulated engine heat-flux conditions will be discussed in conjunction with the coating sintering and fracture testing results.

Design to monitor trend in abundance and presence of American beaver (Castor canadensis) at the national forest scale.

PubMed

Beck, Jeffrey L; Dauwalter, Daniel C; Gerow, Kenneth G; Hayward, Gregory D

2010-05-01

Wildlife conservationists design monitoring programs to assess population dynamics, project future population states, and evaluate the impacts of management actions on populations. Because agency mandates and conservation laws call for monitoring data to elicit management responses, it is imperative to design programs that match the administrative scale for which management decisions are made. We describe a program to monitor population trends in American beaver (Castor canadensis) on the US Department of Agriculture, Black Hills National Forest (BHNF) in southwestern South Dakota and northeastern Wyoming, USA. Beaver have been designated as a management indicator species on the BHNF because of their association with riparian and aquatic habitats and its status as a keystone species. We designed our program to monitor the density of beaver food caches (abundance) within sampling units with beaver and the proportion of sampling units with beavers present at the scale of a national forest. We designated watersheds as sampling units in a stratified random sampling design that we developed based on habitat modeling results. Habitat modeling indicated that the most suitable beaver habitat was near perennial water, near aspen (Populus tremuloides) and willow (Salix spp.), and in low gradient streams at lower elevations. Results from the initial monitoring period in October 2007 allowed us to assess costs and logistical considerations, validate our habitat model, and conduct power analyses to assess whether our sampling design could detect the level of declines in beaver stated in the monitoring objectives. Beaver food caches were located in 20 of 52 sampled watersheds. Monitoring 20 to 25 watersheds with beaver should provide sufficient power to detect 15-40% declines in the beaver food cache index as well as a twofold decline in the odds of beaver being present in watersheds. Indices of abundance, such as the beaver food cache index, provide a practical measure of population status to conduct long-term monitoring across broad landscapes such as national forests.

Correlation of Aerogravity and BHT Data to Develop a Geothermal Gradient Map of the Northern Western Desert of Egypt using an Artificial Neural Network

NASA Astrophysics Data System (ADS)

Mohamed, Haby S.; Abdel Zaher, Mohamed; Senosy, Mahmoud M.; Saibi, Hakim; El Nouby, Mohamed; Fairhead, J. Derek

2015-06-01

The northern part of the Western Desert of Egypt represents the second most promising area of hydrocarbon potential after the Gulf of Suez province. An artificial neural network (ANN) approach was used to develop a new predictive model for calculation of the geothermal gradients in this region based on gravity and corrected bottom-hole temperature (BHT) data. The best training data set was obtained with an ANN architecture composed of seven neurons in the hidden layer, which made it possible to predict the geothermal gradient with satisfactory efficiency. The BHT records of 116 deep oil wells (2,000-4,500 m) were used to evaluate the geothermal resources in the northern Western Desert. Corrections were applied to the BHT data to obtain the true formation equilibrium temperatures, which can provide useful constraints on the subsurface thermal regime. On the basis of these corrected data, the thermal gradient was computed for the linear sections of the temperature-versus-depth data at each well. The calculated geothermal gradient using temperature log data was generally 30 °C/km, with a few local high geothermal gradients in the northwestern parts of the study area explained by potential local geothermal fields. The Bouguer gravity values from the study area ranged from -60 mGal in the southern parts to 120 mGal in the northern areas, and exhibited NE-SW and E-W trends associated with geological structures. Although the northern Western Desert of Egypt has low regional temperature gradients (30 °C/km), several potential local geothermal fields were found (>40 °C/km). The heat flow at each well was also computed by combining sets of temperature gradients and thermal conductivity data. Aerogravity data were used to delineate the subsurface structures and tectonic framework of the region. The result of this study is a new geothermal gradient map of the northern Western Desert developed from gravity and BHT log data.

Instream coliform gradients in the Holtemme, a small headwater stream in the Elbe River Basin, Northern Germany

NASA Astrophysics Data System (ADS)

Karthe, Daniel; Lin, Pei-Ying; Westphal, Katja

2017-09-01

The Holtemme is a small headwater stream in North Germany's Elbe River Basin. According to German and European legislation, hygienic monitoring is not mandatory for such water bodies which are neither drinking water sources nor categorized as bathing waters. Consequently, relatively little is known about the occurrence of-potentially pathogenic-bacteria and viruses in Germany's streams and rivers. The Holtemme was selected for a case study because it is relatively well monitored for both chemical water quality and aquatic ecology, but not for hygiene. Originating in the mountains of Harz Nature Park, the 47 km long Holtemme is characterized by a strong longitudinal gradient in chemical water quality, which is related to different land uses and the influx of treated wastewater from two urban areas (Wernigerode and Halberstadt). Waste water loads received by the Holtemme are comparatively high when compared to similarly small streams. In 2015, total coliform concentrations between more than 200 and 77,010 bacteria per 100 mL, and fecal coliform concentrations between 5 and 24,060 bacteria per 100 mL were observed in the Holtemme's main channel. The highest concentrations were typically found below the outlets of the two wastewater treatment plants. The treated wastewater contained total and fecal coliform concentrations of up to 200,500 and 83,100 per 100 mL, respectively; however, there were significant temporal variations. While the observed concentrations are unproblematic from a legal perspective (because no maximum permissible limits are defined for streams in Germany), they would exceed the tolerable limits for bathing waters in the EU, indicating moderate to critical pollution limits.

Ratiometric Imaging of Extracellular pH in Dental Biofilms.

PubMed

Schlafer, Sebastian; Dige, Irene

2016-03-09

The pH in bacterial biofilms on teeth is of central importance for dental caries, a disease with a high worldwide prevalence. Nutrients and metabolites are not distributed evenly in dental biofilms. A complex interplay of sorption to and reaction with organic matter in the biofilm reduces the diffusion paths of solutes and creates steep gradients of reactive molecules, including organic acids, across the biofilm. Quantitative fluorescent microscopic methods, such as fluorescence life time imaging or pH ratiometry, can be employed to visualize pH in different microenvironments of dental biofilms. pH ratiometry exploits a pH-dependent shift in the fluorescent emission of pH-sensitive dyes. Calculation of the emission ratio at two different wavelengths allows determining local pH in microscopic images, irrespective of the concentration of the dye. Contrary to microelectrodes the technique allows monitoring both vertical and horizontal pH gradients in real-time without mechanically disturbing the biofilm. However, care must be taken to differentiate accurately between extra- and intracellular compartments of the biofilm. Here, the ratiometric dye, seminaphthorhodafluor-4F 5-(and-6) carboxylic acid (C-SNARF-4) is employed to monitor extracellular pH in in vivo grown dental biofilms of unknown species composition. Upon exposure to glucose the dye is up-concentrated inside all bacterial cells in the biofilms; it is thus used both as a universal bacterial stain and as a marker of extracellular pH. After confocal microscopic image acquisition, the bacterial biomass is removed from all pictures using digital image analysis software, which permits to exclusively calculate extracellular pH. pH ratiometry with the ratiometric dye is well-suited to study extracellular pH in thin biofilms of up to 75 µm thickness, but is limited to the pH range between 4.5 and 7.0.

Bubble Formation and Transport during Microgravity Materials Processing: Model Experiments on the Space Station

NASA Technical Reports Server (NTRS)

Grugel, R. N.; Anilkumar, A. V.; Lee, C. P.

2003-01-01

Flow Visualization experiments on the controlled melting and solidification of succinonitrile were conducted in the glovebox facility of the International Space Station (ISS). The experimental samples were prepared on ground by filling glass tubes, 1 cm ID and approximately 30 cm in length, with pure succinonitrile (SCN) under 450 millibar of nitrogen. Porosity in the samples arose from natural shrinkage, and in some cases by direct insertion of nitrogen bubbles, during solidification of the liquid SCN. The samples were processed in the Pore Formation and Mobility Investigation (PFMI) apparatus that is placed in the glovebox facility (GBX) aboard the ISS. Experimental processing parameters of temperature gradient and translation speed, as well as camera settings, were remotely monitored and manipulated from the ground Telescience Center (TSC) at the Marshall Space Flight Center. During the experiments, the sample is first subjected to a unidirectional melt back, generally at 10 microns per second, with a constant temperature gradient ahead of the melting interface. The temperatures in the sample are monitored by six in situ thermocouples. Real time visualization of the controlled directional melt back shows bubbles of different sizes initiating at the melt interface and, upon dislodging from the melting solid, migrating at different speeds into the temperature field ahead of them, before coming to rest. The thermocapillary flow field set up in the melt, ahead of the interface, is dramatic in the context of the large bubbles, and plays a major role in dislodging the bubble. A preliminary analysis of the observed bubble formation and mobility during melt back and its implication to future microgravity experiments is presented and discussed.

Bubble Formation and Transport during Microgravity Materials Processing: Model Experiments on the International Space Station

NASA Technical Reports Server (NTRS)

Grugel, R. N.; Anilkumar, A. V.; Lee, C. P.

2003-01-01

Flow Visualization experiments on the controlled melting and solidification of succinonitrile were conducted in the glovebox facility of the International Space Station (ISS). The experimental samples were prepared on ground by filling glass tubes, 1 cm ID and approximately 30 cm in length, with pure succinonitrile (SCN) under 450 millibar of nitrogen. Porosity in the samples arose from natural shrinkage, and in some cases by direct insertion of nitrogen bubbles, during solidification of the liquid SCN. The samples were processed in the Pore Formation and Mobility Investigation (PFMI) apparatus that is placed in the glovebox facility (GBX) aboard the ISS. Experimental processing parameters of temperature gradient and translation speed, as well as camera settings, were remotely monitored and manipulated from the ground Telescience Center (TSC) at the Marshall Space Flight Center. During the experiments, the sample is first subjected to a unidirectional melt back, generally at 10 microns per second, with a constant temperature gradient ahead of the melting interface. The temperatures in the sample are monitored by six in situ thermocouples. Real time visualization of the controlled directional melt back shows bubbles of different sizes initiating at the melt interface and, upon dislodging from the melting solid, migrating at different speeds into the temperature field ahead of them, before coming to rest. The thermocapillary flow field set up in the melt, ahead of the interface, is dramatic in the context of the large bubbles, and plays a major role in dislodging the bubble. A preliminary analysis of the observed bubble formation and mobility during melt back and its implication to future microgravity experiments is presented and discussed.

Cover of coastal vegetation as an indicator of eutrophication along environmental gradients.

PubMed

Wikström, Sofia A; Carstensen, Jacob; Blomqvist, Mats; Krause-Jensen, Dorte

2016-01-01

Coastal vegetation communities are important for primary production, biodiversity, coastal protection, carbon and nutrient cycling which, in combination with their sensitivity to eutrophication, render them potential indicators of environmental status for environmental policies like the EU Water and Marine Strategy Framework Directives. We evaluated one potential indicator for coastal vegetation, the cumulative cover at depths where the vegetation is light limited, by investigating its response to eutrophication along gradients in natural conditions. We used a large data set covering the Swedish coastline, spanning broad gradients in nutrient level, water clarity, seabed substrate, physical exposure and climate in addition to a salinity gradient from 0.5 to 30.5. Macroalgal cover increased significantly along gradients of declining nutrient concentration and increasing water clarity when we had accounted for diver effects, spatio-temporal sampling variability, salinity gradients, wave exposure and latitude. The developed empirical model explained 79% of the variation in algal cover across 130 areas. Based on this, we identified macroalgal cover as a promising indicator across the Baltic Sea, Kattegat and Skagerrak. A parallel analysis of soft-substrate macrophytes similarly identified significant increases in cover with decreasing concentrations of total nitrogen and increasing salinity, but the resulting empirical model explained only 52% of the variation in cover, probably due to the spatially more variable nature of soft-substrate vegetation. The identified general responses of vegetation cover to gradients of eutrophication across wide ranges in environmental settings may be useful for monitoring and management of marine vegetation in areas with strong environmental gradients.

A ToF-SIMS and XPS study of protein adsorption and cell attachment across PEG-like plasma polymer films with lateral compositional gradients

NASA Astrophysics Data System (ADS)

Menzies, Donna J.; Jasieniak, Marek; Griesser, Hans J.; Forsythe, John S.; Johnson, Graham; McFarland, Gail A.; Muir, Benjamin W.

2012-12-01

In this work we report a detailed X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) study of poly(ethylene glycol) PEG-like chemical gradients deposited via plasma enhanced chemical vapour deposition (PECVD) at two different load powers using diethylene glycol dimethyl ether (DG) as a monomer. Principal component analysis (PCA) was applied to the ToF-SIMS data both before and after protein adsorption on the plasma polymer thin films. Results of the PCA loadings indicated a higher content of hydrocarbon fragments across the higher load power gradient, which adsorbed higher amounts of proteins. Gradients deposited at a lower load power retained a higher degree of monomer like functionality as did the central region directly underneath the knife edge electrode. Analysis of the adsorption of serum proteins (human serum albumin and fetal bovine serum) was monitored across the gradient films and increased with decreasing ether (PEG-like) film chemistries. The effect of protein incubation time on the levels adsorbed fetal bovine serum on the plasma polymer films was critical, with significantly more protein adsorbing after 24 hour incubation times on both gradient films. The attachment of HeLa cells on the gradients appeared to be dictated not only by the surface chemistry, but also by the adsorption of serum proteins. XPS analysis revealed that at surface ether concentrations of less than 70% in the gradient films, significant increases in protein and cell attachment were observed.

The Independent Gradient Model: A New Approach for Probing Strong and Weak Interactions in Molecules from Wave Function Calculations.

PubMed

Lefebvre, Corentin; Khartabil, Hassan; Boisson, Jean-Charles; Contreras-García, Julia; Piquemal, Jean-Philip; Hénon, Eric

2018-03-19

Extraction of the chemical interaction signature from local descriptors based on electron density (ED) is still a fruitful field of development in chemical interpretation. In a previous work that used promolecular ED (frozen ED), the new descriptor, δg , was defined. It represents the difference between a virtual upper limit of the ED gradient (∇ρIGM , IGM=independent gradient model) that represents a noninteracting system and the true ED gradient (∇ρ ). It can be seen as a measure of electron sharing brought by ED contragradience. A compelling feature of this model is to provide an automatic workflow that extracts the signature of interactions between selected groups of atoms. As with the noncovalent interaction (NCI) approach, it provides chemists with a visual understanding of the interactions present in chemical systems. ∇ρIGM is achieved simply by using absolute values upon summing the individual gradient contributions that make up the total ED gradient. Hereby, we extend this model to relaxed ED calculated from a wave function. To this end, we formulated gradient-based partitioning (GBP) to assess the contribution of each orbital to the total ED gradient. We highlight these new possibilities across two prototypical examples of organic chemistry: the unconventional hexamethylbenzene dication, with a hexa-coordinated carbon atom, and β-thioaminoacrolein. It will be shown how a bond-by-bond picture can be obtained from a wave function, which opens the way to monitor specific interactions along reaction paths. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Groundwater Fate and Transport Modeling for Texarkana Wood Preserving Company Superfund Site, Texarkana, Texas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arnett, Ronald Chester

Fate and transport model results are presented for the Texarkana Wood Preserving Company (TWPC)superfund site. The conceptual model assumes two sources of contamination, specifically, the areas around the old and new process areas. Recent data show the presence of non-aqueous phase liquids (NAPL) in the aquifer that are also sources of dissolved contamination in the aquifer. A flow model was constructed and calibrated against measured hydraulic heads at permanent monitoring wells. Good matches were obtained between model simulated heads and most measured heads. An unexplained exception occurs at monitoring well MW-13 down gradient of the site beyond the measured contaminantmore » plume where the model predicts heads that are more than 2 ft. lower than reported field measurements. Adjusting hydraulic parameters in the model could not account for this anomaly and still preserve the head matches at other wells. There is likely a moderate deficiency in the conceptual model or perhaps a data error. Other information such as substantial amounts of infiltrating surface water in the area or a correction in surveyed elevation would improve the flow model. A particle tracking model calculated a travel time from the new process area to the Day’s Creek discharge location on the order of 40 years. Travel times from the old process area to Day’s Creek were calculated to be on the order of 80 years. While these calculations are subject to some uncertainty, travel times of decades are indicated.« less

Real-time quantitation of internal metabolic activity of three-dimensional engineered tissues using an oxygen microelectrode and optical coherence tomography.

PubMed

Kagawa, Yuki; Haraguchi, Yuji; Tsuneda, Satoshi; Shimizu, Tatsuya

2017-05-01

Recent progress in tissue engineering technology has enabled us to develop thick tissue constructs that can then be transplanted in regenerative therapies. In clinical situations, it is vital that the engineered tissues to be implanted are safe and functional before use. However, there is currently a limited number of studies on real-time quality evaluation of thick living tissue constructs. Here we developed a system for quantifying the internal activities of engineered tissues, from which we can evaluate its quality in real-time. The evaluation was achieved by measuring oxygen concentration profiles made along the vertical axis and the thickness of the tissues estimated from cross-sectional images obtained noninvasively by an optical coherence tomography system. Using our novel system, we obtained (i) oxygen concentration just above the tissues, (ii) gradient of oxygen along vertical axis formed above the tissues within culture medium, and (iii) gradient of oxygen formed within the tissues in real-time. Investigating whether these three parameters could be used to evaluate engineered tissues during culturing, we found that only the third parameter was a good candidate. This implies that the activity of living engineered tissues can be monitored in real-time by measuring the oxygen gradient within the tissues. The proposed measuring strategy can be applied to developing more efficient culturing methods to support the fabrication of engineered thick tissues, as well as providing methods to confirm the quality in real-time. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 855-864, 2017. © 2015 Wiley Periodicals, Inc.

Ionospheric tomography by gradient-enhanced kriging with STEC measurements and ionosonde characteristics

NASA Astrophysics Data System (ADS)

Minkwitz, David; van den Boogaart, Karl Gerald; Gerzen, Tatjana; Hoque, Mainul; Hernández-Pajares, Manuel

2016-11-01

The estimation of the ionospheric electron density by kriging is based on the optimization of a parametric measurement covariance model. First, the extension of kriging with slant total electron content (STEC) measurements based on a spatial covariance to kriging with a spatial-temporal covariance model, assimilating STEC data of a sliding window, is presented. Secondly, a novel tomography approach by gradient-enhanced kriging (GEK) is developed. Beyond the ingestion of STEC measurements, GEK assimilates ionosonde characteristics, providing peak electron density measurements as well as gradient information. Both approaches deploy the 3-D electron density model NeQuick as a priori information and estimate the covariance parameter vector within a maximum likelihood estimation for the dedicated tomography time stamp. The methods are validated in the European region for two periods covering quiet and active ionospheric conditions. The kriging with spatial and spatial-temporal covariance model is analysed regarding its capability to reproduce STEC, differential STEC and foF2. Therefore, the estimates are compared to the NeQuick model results, the 2-D TEC maps of the International GNSS Service and the DLR's Ionospheric Monitoring and Prediction Center, and in the case of foF2 to two independent ionosonde stations. Moreover, simulated STEC and ionosonde measurements are used to investigate the electron density profiles estimated by the GEK in comparison to a kriging with STEC only. The results indicate a crucial improvement in the initial guess by the developed methods and point out the potential compensation for a bias in the peak height hmF2 by means of GEK.

Single-laboratory validation of a high-performance liquid chromatographic-diode array detector-fluorescence detector/mass spectrometric method for simultaneous determination of water-soluble vitamins in multivitamin dietary tablets.

PubMed

Chen, Pei; Atkinson, Renata; Wolf, Wayne R

2009-01-01

The purpose of this study was to develop a single-laboratory validated (SLV) method using high-performance liquid chromatography with different detectors [diode array detector (DAD); fluorescence detector (FLD); and mass spectrometry (MS)] for determination of 7 B-complex vitamins (B1-thiamin, B2-riboflavin, B3-nicotinamide, B6-pyridoxine, B9-folic acid, pantothenic acid, and biotin) and vitamin C in multivitamin/multimineral dietary supplements. The method involves the use of a reversed-phase octadecylsilyl column (4 microm, 250 x 2.0 mm id) and a gradient mobile phase profile. Gradient elution was performed at a flow rate of 0.25 mL/min. After a 5 min isocratic elution at 100% A (0.1% formic acid in water), a linear gradient to 50% A and 50% B (0.1% formic acid in acetonitrile) at 15 min was employed. Detection was performed with a DAD as well as either an FLD or a triple-quadrupole MS detector in the multiple reaction monitoring mode. SLV was performed using Standard Reference Material (SRM) 3280 Multivitamin/Multimineral Tablets, being developed by the National Institute of Standards and Technology, with support by the Office of Dietary Supplements of the National Institutes of Health. Phosphate buffer (10 mM, pH 2.0) extracts of the NIST SRM 3280 were analyzed by the liquid chromatographic (LC)-DAD-FLDIMS method. Following extraction, the method does not require any sample cleanup/preconcentration steps except centrifugation and filtration.

Single-Laboratory Validation of a High-Performance Liquid Chromatographic-Diode Array Detector-Fluorescence Detector/Mass Spectrometric Method for Simultaneous Determination of Water-Soluble Vitamins in Multivitamin Dietary Tablets

PubMed Central

Chen, Pei; Atkinson, Renata; Wolf, Wayne R.

2014-01-01

The purpose of this study was to develop a single-laboratory validated (SLV) method using high-performance liquid chromatography with different detectors [diode array detector (DAD); fluorescence detector (FLD); and mass spectrometry (MS)] for determination of 7 B-complex vitamins (B1-thiamin, B2-riboflavin, B3-nicotinamide, B6-pyridoxine, B9-folic acid, pantothenic acid, and biotin) and vitamin C in multivitamin/multimineral dietary supplements. The method involves the use of a reversed-phase octadecylsilyl column (4 µm, 250 × 2.0 mm id) and a gradient mobile phase profile. Gradient elution was performed at a flow rate of 0.25 mL/min. After a 5 min isocratic elution at 100% A (0.1% formic acid in water), a linear gradient to 50% A and 50% B (0.1% formic acid in acetonitrile) at 15 min was employed. Detection was performed with a DAD as well as either an FLD or a triple-quadrupole MS detector in the multiple reaction monitoring mode. SLV was performed using Standard Reference Material (SRM) 3280 Multivitamin/Multimineral Tablets, being developed by the National Institute of Standards and Technology, with support by the Office of Dietary Supplements of the National Institutes of Health. Phosphate buffer (10 mM, pH 2.0) extracts of the NIST SRM 3280 were analyzed by the liquid chromatographic (LC)-DAD-FLD/MS method. Following extraction, the method does not require any sample cleanup/preconcentration steps except centrifugation and filtration. PMID:19485230

Implementing oxygen control in chip-based cell and tissue culture systems.

PubMed

Oomen, Pieter E; Skolimowski, Maciej D; Verpoorte, Elisabeth

2016-09-21

Oxygen is essential in the energy metabolism of cells, as well as being an important regulatory parameter influencing cell differentiation and function. Interest in precise oxygen control for in vitro cultures of tissues and cells continues to grow, especially with the emergence of the organ-on-a-chip and the desire to emulate in vivo conditions. This was recently discussed in this journal in a Critical Review by Brennan et al. (Lab Chip (2014). DOI: ). Microfluidics can be used to introduce flow to facilitate nutrient supply to and waste removal from in vitro culture systems. Well-defined oxygen gradients can also be established. However, cells can quickly alter the oxygen balance in their vicinity. In this Tutorial Review, we expand on the Brennan paper to focus on the implementation of oxygen analysis in these systems to achieve continuous monitoring. Both electrochemical and optical approaches for the integration of oxygen monitoring in microfluidic tissue and cell culture systems will be discussed. Differences in oxygen requirements from one organ to the next are a challenging problem, as oxygen delivery is limited by its uptake into medium. Hence, we discuss the factors determining oxygen concentrations in solutions and consider the possible use of artificial oxygen carriers to increase dissolved oxygen concentrations. The selection of device material for applications requiring precise oxygen control is discussed in detail, focusing on oxygen permeability. Lastly, a variety of devices is presented, showing the diversity of approaches that can be employed to control and monitor oxygen concentrations in in vitro experiments.

Uranium 238U/235U isotope ratios as indicators of reduction: Results from an in situ biostimulation experiment at Rifle, Colorado, USA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bopp IV, C.J.; Lundstrom, C.C.; Johnson, T.M.

2010-02-01

The attenuation of groundwater contamination via chemical reaction is traditionally evaluated by monitoring contaminant concentration through time. However, this method can be confounded by common transport processes (e.g. dilution, sorption). Isotopic techniques bypass the limits of concentration methods, and so may provide improved accuracy in determining the extent of reaction. We apply measurements of {sup 238}U/{sup 235}U to a U bioremediation field experiment at the Rifle Integrated Field Research Challenge Site in Rifle, Colorado (USA). An array of monitoring and injection wells was installed on a 100 m{sup 2} plot where U(VI) contamination was present in the groundwater. Acetate-amended groundwatermore » was injected along an up-gradient gallery to encourage the growth of dissimilatory metal reducing bacteria (e.g. Geobacter species). During amendment, U concentration dropped by an order of magnitude in the experiment plot. We measured {sup 238}U/{sup 235}U in samples from one monitoring well by MC-ICP-MS using a double isotope tracer method. A significant {approx}1.00{per_thousand} decrease in {sup 238}U/{sup 235}U occurred in the groundwater as U(VI) concentration decreased. The relationship between {sup 238}U/{sup 235}U and concentration corresponds approximately to a Rayleigh distillation curve with an effective fractionation factor ({alpha}) of 1.00046. We attribute the observed U isotope fractionation to a nuclear field shift effect during enzymatic reduction of U(VI){sub (aq)} to U(IV){sub (s)}.« less

Field Evaluation of Two Geophysical Techniques for Real-Time Mapping of Smouldering Remediation (STAR)

NASA Astrophysics Data System (ADS)

Trento, L. M.; Tsourlos, P.; McMaster, M.; Liefl, D.; Sims, A.; Dominguez, J. L. G.; Vidumsky, J.; Gerhard, J.

2016-12-01

Self-sustaining Treatment for Active Remediation (STAR) technology destroys non-aqueous phase liquid (NAPL) in situ using principles of smouldering combustion. It involves propagating an exothermic (400-1000C) oxidation reaction outwards from an ignition well. A full-scale STAR system is currently being applied at an industrial site contaminated with coal tar below the water table in New Jersey, USA. STAR is typically tracked using multi-level thermocouples, which are discrete and sparse in space and time. This study evaluates two surface-based geophysical methods - Electrical Resistivity Tomography (ERT) and Self-Potential (SP) - for the ability to map the STAR reaction in real time at the New Jersey site. Both techniques involve placing electrode arrays on the surface and monitoring electrical signals over time (i.e., time-lapse). It is hypothesized that ERT should be able to monitor the resistive dry zone that precedes the reaction front and/or the growing NAPL-depleted zone. SP is expected to be able to detect the potential difference associated with thermal gradients generated by the reaction. Approximately 72 ERT electrodes in a "swiss cross" pattern plus 10 SP electrodes will be emplaced over single STAR treatment cell (six ignition wells). This setup will be employed to monitor both a deep (25 feet) and shallow (8 feet) STAR treatments. The geophysics will be complemented by in situ temperature measurements, continuous gas measurements, and pre- and post-treatment coring. The primary goal of this research is to evaluate the effectiveness of using ERT and SP for STAR under field conditions. The tests will be conducted in August 2016.

Utilizing NASA DISCOVER-AQ Data to Examine Spatial Gradients in Complex Emission Environments

NASA Astrophysics Data System (ADS)

Buzanowicz, M. E.; Moore, W.; Crawford, J. H.; Schroeder, J.

2017-12-01

Although many regulations have been enacted with the goal of improving air quality, many parts of the US are still classified as `non-attainment areas' because they frequently violate federal air quality standards. Adequately monitoring the spatial distribution of pollutants both within and outside of non-attainment areas has been an ongoing challenge for regulators. Observations of near-surface pollution from space-based platforms would provide an unprecedented view of the spatial distribution of pollution, but this goal has not yet been realized due to fundamental limitations of satellites, specifically because the footprint size of satellite measurements may not be sufficiently small enough to capture true gradients in pollution, and rather represents an average over a large area. NASA's DISCOVER-AQ was a multi-year field campaign aimed at improving our understanding of the role that remote sensing, including satellite-based remote sensing, could play in air quality monitoring systems. DISCOVER-AQ data will be utilized to create a metric to examine spatial gradients and how satellites can capture those gradients in areas with complex emission environments. Examining horizontal variability within a vertical column is critical to understanding mixing within the atmosphere. Aircraft spirals conducted during DISCOVER-AQ were divided into octants, and averages of a given a species were calculated, with certain points receiving a flag. These flags were determined by calculating gradients between subsequent octants. Initial calculations have shown that over areas with large point source emissions, such as Platteville and Denver-La Casa in Colorado, and Essex, Maryland, satellite retrievals may not adequately capture spatial variability in the atmosphere, thus complicating satellite inversion techniques and limiting our ability to understand human exposure on sub-grid scales. Further calculations at other locations and for other trace gases are necessary to determine the effects of vertical variability within the atmosphere.

Vertical gradients in water chemistry in the central High Plains aquifer, southwestern Kansas and Oklahoma panhandle, 1999

USGS Publications Warehouse

McMahon, Peter B.

2001-01-01

The central High Plains aquifer is the primary source of water for domestic, industrial, and irrigation uses in parts of Colorado, Kansas, New Mexico, Oklahoma, and Texas. Water-level declines of more than 100 feet in some areas of the aquifer have increased the demand for water deeper in the aquifer. The maximum saturated thickness of the aquifer ranged from 500 to 600 feet in 1999. As the demand for deeper water increases, it becomes increasingly important for resource managers to understand how the quality of water in the aquifer changes with depth. In 1998?99, 18 monitoring wells at nine sites in southwestern Kansas and the Oklahoma Panhandle were completed at various depths in the central High Plains aquifer, and one monitoring well was completed in sediments of Permian age underlying the aquifer. Water samples were collected once from each well in 1999 to measure vertical gradients in water chemistry in the aquifer. Tritium concentrations measured in ground water indicate that water samples collected in the upper 30 feet of the aquifer were generally recharged within the last 50 years, whereas all of the water samples collected at depths more than 30 feet below the water table were recharged more than 50 years ago. Dissolved oxygen was present throughout the aquifer, with concentrations ranging from 1.7 to 8.4 mg/L. Water in the central High Plains aquifer was predominantly a calcium-bicarbonate type that exhibited little variability in concentrations of dissolved solids with depth (290 to 642 mg/L). Exceptions occurred in some areas where there had been upward movement of mineralized water from underlying sediments of Permian age and areas where there had been downward movement of mineralized Arkansas River water to the aquifer. Calcium-sulfate and sodium-chloride waters dominated and concentrations of dissolved solids were elevated (862 to 4,030 mg/L) near the base of the aquifer in the areas of upward leakage. Dissolution of gypsum or anhydrite and halite in sediments of Permian age by ground water was the likely source of calcium, sulfate, sodium, and chloride in those waters. Calcium-sodium-sulfate waters dominated, and concentrations of dissolved solids were as large as 4,916 mg/L near the water table in the area of downward leakage. Dissolution of minerals in sedimentary deposits of marine origin in upstream areas of the Arkansas River drainage were the likely sources of calcium, sodium, and sulfate in those waters. Nitrate was detected throughout the aquifer and the background concentration was estimated to be 2.45 mg/L as N. The largest nitrate concentrations (8.28, 22, and 54.4 mg/L as N) occurred in recently recharged water collected adjacent to irrigated fields. Three pesticides (atrazine, metolachlor, simazine) and five pesticide degradation products (alachlor ethanesulfonic acid, alachlor oxanilic acid, deethylatrazine, metolachlor ethanesulfonic acid, metolachlor oxanilic acid) were detected in recently recharged water from six water-table wells. Five of the six wells were adjacent to irrigated fields. These data indicate that concentrations of nitrate and pesticides increased over time in some areas of the aquifer as a result of agricultural activities. Results from this study indicate that vertical gradients in water chemistry existed in the central High Plains aquifer. The chemical gradients resulted from chemical inputs to the aquifer from underlying sediments of Permian age, from the Arkansas River, and from agricultural activities. In areas where those chemical inputs occurred, water quality in the aquifer was impaired and may not have been suitable for some intended uses.

Towards an integrated soil moisture drought monitor for East Africa

USDA-ARS?s Scientific Manuscript database

Drought in East Africa is a recurring phenomenon with significant humanitarian impacts. Given the steep climatic gradients, topographic contrasts, general data scarcity, and, in places, political instability that characterize the region, there is a need for spatially distributed, remotely derived mo...

Stable isotope differences among the Lake Michigan 2015 CSMI transects

EPA Science Inventory

During the Lake Michigan 2015 Cooperative Science and Monitoring Initiative (CSMI), eight transects situated near tributaries that present a gradient of phosphorus loads were sampled from nearshore to offshore during May, July, and September. Our objective was to evaluate associa...

Monitoring of fluid motion in a micromixer by dynamic NMR microscopy.

PubMed

Ahola, Susanna; Casanova, Federico; Perlo, Juan; Münnemann, Kerstin; Blümich, Bernhard; Stapf, Siegfried

2006-01-01

The velocity distribution of liquid flowing in a commercial micromixer has been determined directly by using pulsed-field gradient NMR. Velocity maps with a spatial resolution of 29 microm x 43 microm were obtained by combining standard imaging gradient units with a homebuilt rectangular surface coil matching the mixer geometry. The technique provides access to mixers and reactors of arbitrary shape regardless of optical transparency. Local heterogeneities in the signal intensity and the velocity pattern were found and serve to investigate the quality and functionality of a micromixer, revealing clogging and inhomogeneous flow distributions.

Unsteady Oxygen Transfer in Space-Filling Models of the Pulmonary Acinus

NASA Astrophysics Data System (ADS)

Hofemeier, Philipp; Shachar-Berman, Lihi; Filoche, Marcel; Sznitman, Josue

2014-11-01

Diffusional screening in the pulmonary acinus is a well-known physical phenomenon that results from the depletion of fresh oxygen in proximal acinar generations diffusing through the alveolar wall membranes and effectively creating a gradient in the oxygen partial pressure along the acinar airways. Until present, most studies have focused on steady-state oxygen diffusion in generic sub-acinar structures and discarded convective oxygen transport due to low Peclet numbers in this region. Such studies, however, fall typically short in capturing the complex morphology of acinar airways as well as the oscillatory nature of convecive acinar breathing. Here, we revisit this problem and solve the convective-diffusive transport equations in breathing 3D acinar structures, underlining the significance of convective flows in proximal acinar generations as well as recirculating alveolar flow patterns. In particular, to assess diffusional screening, we monitor time-dependent efficiencies of the acinus under cyclic breathing motion. Our study emphasizes the necessity of capturing both a dynamically breathing and anatomically-realistic model of the sub-acinus to characterize unsteady oxygen transport across the acinar walls.

Erroneous intracranial pressure measurements from simultaneous pressure monitoring and ventricular drainage catheters.

PubMed

Birch, A A; Eynon, C A; Schley, D

2006-01-01

The objective of this report is to highlight the potential for false pressure measurements from systems that combine intracranial pressure (ICP) measurement and ventricular drainage. If the ports of the drain become blocked to the extent that they present a high resistance to cerebrospinal fluid flow, then a significant pressure gradient between the inside and outside of the catheter may be established. Thus, any intracatheter transducer will faithfully record a pressure much lower than true ICP. This holds true for catheter-tip transducers when the transducer lies inside the catheter. In the absence of flow, however, pressures will equalize; therefore, accurate measurements may be taken if the drain is temporarily closed. We model this situation and provide simulations of expected measurements in such situations; these compare well to observed clinical readings.

An optically coupled system for quantitative monitoring of MRI gradient currents induced into endocardial leads.

PubMed

Mattei, E; Calcagnini, G; Triventi, M; Delogu, A; Del Guercio, M; Angeloni, A; Bartolini, P

2013-01-01

The time-varying gradient fields generated during Magnetic Resonance Imaging (MRI) procedures have the potential to induce electrical current on implanted endocardial leads. Whether this current can result in undesired cardiac stimulation is unknown. This paper presents an optically coupled system with the potential to quantitatively measure the currents induced by the gradient fields into endocardial leads during MRI procedures. Our system is based on a microcontroller that works as analog-to-digital (A/D) converter and sends the current signal acquired from the lead to an optical high-speed light-emitting-diode transmitter. Plastic fiber guides the light outside the MRI chamber, to a photodiode receiver and then to an acquisition board connected to a PC. The preliminary characterization of the performances of the system is also presented.

Variation of cosmic rays and solar wind properties with respect to the heliospheric current sheet. II - Rigidity dependence of the latitudinal gradient of cosmic rays at 1 AU

NASA Technical Reports Server (NTRS)

Newkirk, G., Jr.; Asbridge, J.; Lockwood, J. A.; Garcia-Munoz, M.; Simpson, J. A.

1986-01-01

The role which empirical determinations of the latitudinal variation of cosmic rays with respect to the current sheet may have in illuminating the importance of the cross-field drift of particles in the large-scale heliospheric magnetic field is discussed. Using K coronameter observations and measured solar wind speeds, the latitudinal gradients have been determined with respect to the current sheet for cosmic rays in four rigidity ranges. Gradients vary between approximately -2 and -50 pct/AU. The rigidity dependence of the decrease of cosmic ray flux with distance from the current sheet lies between the -0.72 to -0.86 power of the rigidity, with the exact dependence being determined by the definition used for the median rigidity of each monitor.

Field evaluations of newly available "interference-free" monitors for nitrogen dioxide and ozone at near-road and conventional National Ambient Air Quality Standards compliance sites.

PubMed

Leston, Alan R; Ollison, Will M

2017-11-01

Long-standing measurement techniques for determining ground-level ozone (O 3 ) and nitrogen dioxide (NO 2 ) are known to be biased by interfering compounds that result in overestimates of high O 3 and NO 2 ambient concentrations under conducive conditions. An increasing near-ground O 3 gradient (NGOG) with increasing height above ground level is also known to exist. Both the interference bias and NGOG were investigated by comparing data from a conventional Federal Equivalent Method (FEM) O 3 photometer and an identical monitor upgraded with an "interference-free" nitric oxide O 3 scrubber that alternatively sampled at 2 m and 6.2 m inlet heights above ground level (AGL). Intercomparison was also made between a conventional nitrogen oxide (NO x ) chemiluminescence Federal Reference Method (FRM) monitor and a new "direct-measure" NO 2 NO x 405 nm photometer at a near-road air quality measurement site. Results indicate that the O 3 monitor with the upgraded scrubber recorded lower regulatory-oriented concentrations than the deployed conventional metal oxide-scrubbed monitor and that O 3 concentrations 6.2 m AGL were higher than concentrations 2.0 m AGL, the nominal nose height of outdoor populations. Also, a new direct-measure NO 2 photometer recorded generally lower NO 2 regulatory-oriented concentrations than the conventional FRM chemiluminescence monitor, reporting lower daily maximum hourly average concentrations than the conventional monitor about 3 of every 5 days. Employing bias-prone instruments for measurement of ambient ozone or nitrogen dioxide from inlets at inappropriate heights above ground level may result in collection of positively biased data. This paper discusses tests of new regulatory instruments, recent developments in bias-free ozone and nitrogen dioxide measurement technology, and the presence/extent of a near-ground O 3 gradient (NGOG). Collection of unbiased monitor inlet height-appropriate data is crucial for determining accurate design values and meeting National Ambient Air Quality Standards.

Leatherback Turtle Movements, Dive Behavior, and Habitat Characteristics in Ecoregions of the Northwest Atlantic Ocean

PubMed Central

Dodge, Kara L.; Galuardi, Benjamin; Miller, Timothy J.; Lutcavage, Molly E.

2014-01-01

Leatherback sea turtles, Dermochelys coriacea, are highly migratory predators that feed exclusively on gelatinous zooplankton, thus playing a unique role in coastal and pelagic food webs. From 2007 to 2010, we used satellite telemetry to monitor the movements and dive behavior of nine adult and eleven subadult leatherbacks captured on the Northeast USA shelf and tracked throughout the Northwest Atlantic. Leatherback movements and environmental associations varied by oceanographic region, with slow, sinuous, area-restricted search behavior and shorter, shallower dives occurring in cool (median sea surface temperature: 18.4°C), productive (median chlorophyll a: 0.80 mg m−3), shallow (median bathymetry: 57 m) shelf habitat with strong sea surface temperature gradients (median SST gradient: 0.23°C km−1) at temperate latitudes. Leatherbacks were highly aggregated in temperate shelf and slope waters during summer, early fall, and late spring and more widely dispersed in subtropical and tropical oceanic and neritic habitat during late fall, winter and early spring. We investigated the relationship of ecoregion, satellite-derived surface chlorophyll, satellite-derived sea surface temperature, SST gradient, chlorophyll gradient and bathymetry with leatherback search behavior using generalized linear mixed-effects models. The most well supported model showed that differences in leatherback search behavior were best explained by ecoregion and regional differences in bathymetry and SST. Within the Northwest Atlantic Shelves region, leatherbacks increased path sinuosity (i.e., looping movements) with increasing SST, but this relationship reversed within the Gulf Stream region. Leatherbacks increased path sinuosity with decreasing water depth in temperate and tropical shelf habitats. This relationship is consistent with increasing epipelagic gelatinous zooplankton biomass with decreasing water depth, and bathymetry may be a key feature in identifying leatherback foraging habitat in neritic regions. High-use habitat for leatherbacks in our study occurred in coastal waters of the North American eastern seaboard and eastern Caribbean, putting turtles at heightened risk from land- and ocean-based human activity. PMID:24646920

Benchmark Campaign of the COST Action GNSS4SWEC: Main Goals and Achievements

NASA Astrophysics Data System (ADS)

Dick, G.; Dousa, J.; Kacmarik, M.; Pottiaux, E.; Zus, F.; Brenot, H. H.; Moeller, G.; Kaplon, J.; Morel, L.; Hordyniec, P.

2016-12-01

This talk will give an overview of achievements of the Benchmark campaign, one of the central activities in the framework of the COST Action ES 1206 GNSS4SWEC. The main goal of the campaign is supporting the development and validation of advanced Global Navigation Satellite System (GNSS) tropospheric products, in particular high-resolution and ultra-fast/real-time zenith total delays (ZTD) and asymmetry products in terms of tropospheric horizontal gradients and slant delays.For the Benchmark campaign a complex data set of GNSS observations and various meteorological data were collected for a two-month period in 2013 (May-June) which included severe weather events in central Europe. An initial processing of data sets from GNSS and numerical weather models (NWM) provided independently estimated tropospheric reference products - ZTDs, tropospheric horizontal gradients and others. The comparison of horizontal tropospheric gradients from GNSS and NWM data demonstrated a very good agreement among independent solutions with negligible biases and an accuracy of about 0.5 mm. Visual comparisons of maps of zenith wet delays and tropospheric horizontal gradients showed very promising results for future exploitations of advanced GNSS tropospheric products in meteorological applications such as severe weather event monitoring and weather nowcasting.The benchmark data set is also used for an extensive validation of line-of-sight tropospheric Slant Total Delays (STD) from GNSS, NWM-raytracing and Water Vapour Radiometer (WVR) solutions. Six institutions delivered their STDs based on GNSS observations processed using different software and strategies. STDs from NWM ray-tracing came from three institutions using three different NWM models. Results show generally a very good mutual agreement among all solutions from all techniques. Among all an influence of adding not cleaned as well as cleaned GNSS post-fit residuals, i.e. residuals with eliminated and not eliminated non-tropospheric systematic effects such as multipath, to estimated STDs will be presented.

Leatherback turtle movements, dive behavior, and habitat characteristics in ecoregions of the Northwest Atlantic Ocean.

PubMed

Dodge, Kara L; Galuardi, Benjamin; Miller, Timothy J; Lutcavage, Molly E

2014-01-01

Leatherback sea turtles, Dermochelys coriacea, are highly migratory predators that feed exclusively on gelatinous zooplankton, thus playing a unique role in coastal and pelagic food webs. From 2007 to 2010, we used satellite telemetry to monitor the movements and dive behavior of nine adult and eleven subadult leatherbacks captured on the Northeast USA shelf and tracked throughout the Northwest Atlantic. Leatherback movements and environmental associations varied by oceanographic region, with slow, sinuous, area-restricted search behavior and shorter, shallower dives occurring in cool (median sea surface temperature: 18.4°C), productive (median chlorophyll a: 0.80 mg m(-3)), shallow (median bathymetry: 57 m) shelf habitat with strong sea surface temperature gradients (median SST gradient: 0.23°C km(-1)) at temperate latitudes. Leatherbacks were highly aggregated in temperate shelf and slope waters during summer, early fall, and late spring and more widely dispersed in subtropical and tropical oceanic and neritic habitat during late fall, winter and early spring. We investigated the relationship of ecoregion, satellite-derived surface chlorophyll, satellite-derived sea surface temperature, SST gradient, chlorophyll gradient and bathymetry with leatherback search behavior using generalized linear mixed-effects models. The most well supported model showed that differences in leatherback search behavior were best explained by ecoregion and regional differences in bathymetry and SST. Within the Northwest Atlantic Shelves region, leatherbacks increased path sinuosity (i.e., looping movements) with increasing SST, but this relationship reversed within the Gulf Stream region. Leatherbacks increased path sinuosity with decreasing water depth in temperate and tropical shelf habitats. This relationship is consistent with increasing epipelagic gelatinous zooplankton biomass with decreasing water depth, and bathymetry may be a key feature in identifying leatherback foraging habitat in neritic regions. High-use habitat for leatherbacks in our study occurred in coastal waters of the North American eastern seaboard and eastern Caribbean, putting turtles at heightened risk from land- and ocean-based human activity.

Modified conjugate gradient method for diagonalizing large matrices.

PubMed

Jie, Quanlin; Liu, Dunhuan

2003-11-01

We present an iterative method to diagonalize large matrices. The basic idea is the same as the conjugate gradient (CG) method, i.e, minimizing the Rayleigh quotient via its gradient and avoiding reintroducing errors to the directions of previous gradients. Each iteration step is to find lowest eigenvector of the matrix in a subspace spanned by the current trial vector and the corresponding gradient of the Rayleigh quotient, as well as some previous trial vectors. The gradient, together with the previous trial vectors, play a similar role as the conjugate gradient of the original CG algorithm. Our numeric tests indicate that this method converges significantly faster than the original CG method. And the computational cost of one iteration step is about the same as the original CG method. It is suitable for first principle calculations.

Drift and Behavior of E. coli Cells

NASA Astrophysics Data System (ADS)

Micali, Gabriele; Colin, Rémy; Sourjik, Victor; Endres, Robert G.

2017-12-01

Chemotaxis of the bacterium Escherichia coli is well understood in shallow chemical gradients, but its swimming behavior remains difficult to interpret in steep gradients. By focusing on single-cell trajectories from simulations, we investigated the dependence of the chemotactic drift velocity on attractant concentration in an exponential gradient. While maxima of the average drift velocity can be interpreted within analytical linear-response theory of chemotaxis in shallow gradients, limits in drift due to steep gradients and finite number of receptor-methylation sites for adaptation go beyond perturbation theory. For instance, we found a surprising pinning of the cells to the concentration in the gradient at which cells run out of methylation sites. To validate the positions of maximal drift, we recorded single-cell trajectories in carefully designed chemical gradients using microfluidics.

B1 gradient coherence selection using a tapered stripline.

PubMed

van Meerten, S G J; Tijssen, K C H; van Bentum, P J M; Kentgens, A P M

2018-01-01

Pulsed-field gradients are common in modern liquid state NMR pulse sequences. They are often used instead of phase cycles for the selection of coherence pathways, thereby decreasing the time required for the NMR experiment. Soft off-resonance pulses with a B 1 gradient result in a spatial encoding similar to that created by pulsed-field (B 0 ) gradients. In this manuscript we show that pulse sequences with pulsed-field gradients can easily be converted to one which uses off-resonance B 1 field gradient (OFFBEAT) pulses. The advantage of B 1 gradient pulses for coherence selection is that the chemical shift evolution during the pulses is (partially) suppressed. Therefore no refocusing echos are required to correct for evolution during the gradient pulses. A tapered stripline is shown to be a convenient tool for creating a well-defined gradient in the B 1 field strength. B 1 gradient coherence selection using a tapered stripline is a simple and cheap alternative to B 0 pulsed-field gradients. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

Multi-Scale Modification of Metallic Implants With Pore Gradients, Polyelectrolytes and Their Indirect Monitoring In vivo

PubMed Central

Vrana, Nihal E.; Dupret-Bories, Agnes; Chaubaroux, Christophe; Rieger, Elisabeth; Debry, Christian; Vautier, Dominique; Metz-Boutigue, Marie-Helene; Lavalle, Philippe

2013-01-01

Metallic implants, especially titanium implants, are widely used in clinical applications. Tissue in-growth and integration to these implants in the tissues are important parameters for successful clinical outcomes. In order to improve tissue integration, porous metallic implants have being developed. Open porosity of metallic foams is very advantageous, since the pore areas can be functionalized without compromising the mechanical properties of the whole structure. Here we describe such modifications using porous titanium implants based on titanium microbeads. By using inherent physical properties such as hydrophobicity of titanium, it is possible to obtain hydrophobic pore gradients within microbead based metallic implants and at the same time to have a basement membrane mimic based on hydrophilic, natural polymers. 3D pore gradients are formed by synthetic polymers such as Poly-L-lactic acid (PLLA) by freeze-extraction method. 2D nanofibrillar surfaces are formed by using collagen/alginate followed by a crosslinking step with a natural crosslinker (genipin). This nanofibrillar film was built up by layer by layer (LbL) deposition method of the two oppositely charged molecules, collagen and alginate. Finally, an implant where different areas can accommodate different cell types, as this is necessary for many multicellular tissues, can be obtained. By, this way cellular movement in different directions by different cell types can be controlled. Such a system is described for the specific case of trachea regeneration, but it can be modified for other target organs. Analysis of cell migration and the possible methods for creating different pore gradients are elaborated. The next step in the analysis of such implants is their characterization after implantation. However, histological analysis of metallic implants is a long and cumbersome process, thus for monitoring host reaction to metallic implants in vivo an alternative method based on monitoring CGA and different blood proteins is also described. These methods can be used for developing in vitro custom-made migration and colonization tests and also be used for analysis of functionalized metallic implants in vivo without histology. PMID:23851618

Multi-scale modification of metallic implants with pore gradients, polyelectrolytes and their indirect monitoring in vivo.

PubMed

Vrana, Nihal E; Dupret-Bories, Agnes; Chaubaroux, Christophe; Rieger, Elisabeth; Debry, Christian; Vautier, Dominique; Metz-Boutigue, Marie-Helene; Lavalle, Philippe

2013-07-01

Metallic implants, especially titanium implants, are widely used in clinical applications. Tissue in-growth and integration to these implants in the tissues are important parameters for successful clinical outcomes. In order to improve tissue integration, porous metallic implants have being developed. Open porosity of metallic foams is very advantageous, since the pore areas can be functionalized without compromising the mechanical properties of the whole structure. Here we describe such modifications using porous titanium implants based on titanium microbeads. By using inherent physical properties such as hydrophobicity of titanium, it is possible to obtain hydrophobic pore gradients within microbead based metallic implants and at the same time to have a basement membrane mimic based on hydrophilic, natural polymers. 3D pore gradients are formed by synthetic polymers such as Poly-L-lactic acid (PLLA) by freeze-extraction method. 2D nanofibrillar surfaces are formed by using collagen/alginate followed by a crosslinking step with a natural crosslinker (genipin). This nanofibrillar film was built up by layer by layer (LbL) deposition method of the two oppositely charged molecules, collagen and alginate. Finally, an implant where different areas can accommodate different cell types, as this is necessary for many multicellular tissues, can be obtained. By, this way cellular movement in different directions by different cell types can be controlled. Such a system is described for the specific case of trachea regeneration, but it can be modified for other target organs. Analysis of cell migration and the possible methods for creating different pore gradients are elaborated. The next step in the analysis of such implants is their characterization after implantation. However, histological analysis of metallic implants is a long and cumbersome process, thus for monitoring host reaction to metallic implants in vivo an alternative method based on monitoring CGA and different blood proteins is also described. These methods can be used for developing in vitro custom-made migration and colonization tests and also be used for analysis of functionalized metallic implants in vivo without histology.

Effective detection of CO 2 leakage: a comparison of groundwater sampling and pressure monitoring

DOE PAGES

Keating, Elizabeth; Dai, Zhenxue; Dempsey, David; ...

2014-12-31

Shallow aquifer monitoring is likely to be a required aspect to any geologic CO 2 sequestration operation. Collecting groundwater samples and analyzing for geochemical parameters such as pH, alkalinity, total dissolved carbon, and trace metals has been suggested by a number of authors as a possible strategy to detect CO 2 leakage. The effectiveness of this approach, however, will depend on the hydrodynamics of the leak-induced CO 2 plume and the spatial distribution of the monitoring wells relative to the origin of the leak. To our knowledge, the expected effectiveness of groundwater sampling to detect CO 2 leakage has notmore » yet been quantitatively assessed. In this study we query hundreds of simulations developed for the National Risk Assessment Project (US DOE) to estimate risks to drinking water resources associated with CO 2 leaks. The ensemble of simulations represent transient, 3-D multi-phase reactive transport of CO 2 and brine leaked from a sequestration reservoir, via a leaky wellbore, into an unconfined aquifer. Key characteristics of the aquifer, including thickness, mean permeability, background hydraulic gradient, and geostatistical measures of aquifer heterogeneity, were all considered uncertain parameters. Complex temporally-varying CO 2 and brine leak rate scenarios were simulated using a heuristic scheme with ten uncertain parameters. The simulations collectively predict the spatial and temporal evolution of CO 2 and brine plumes over 200 years in a shallow aquifer under a wide range of leakage scenarios and aquifer characteristics. Using spatial data from an existing network of shallow drinking water wells in the Edwards Aquifer, TX, as one illustrative example, we calculated the likelihood of leakage detection by groundwater sampling. In this monitoring example, there are 128 wells available for sampling, with a density of about 2.6 wells per square kilometer. If the location of the leak is unknown a priori, a reasonable assumption in many cases, we found that the leak would be detected in at least one of the monitoring wells in less than 10% of the scenarios considered. This is because plume sizes are relatively small, and so the probability of detection decreases rapidly with distance from the leakage point. For example, 400m away from the leakage point there is less than 20% chance of detection. We then compared the effectiveness of groundwater quality sampling to shallow aquifer and/or reservoir pressure monitoring. For the Edwards Aquifer example, pressure monitoring in the same monitoring well network was found to be even less effective that groundwater quality monitoring. This is presumably due to the unconfined conditions and relatively high permeability, so pressure perturbations quickly dissipate. Although specific results may differ from site to site, this type of analysis should be useful to site operators and regulators when selecting leak detection strategies. Given the spatial characteristics of a proposed monitoring well network, probabilities of leakage detection can be rapidly calculated using this methodology. Although conditions such as these may not be favorable for leakage detection in shallow aquifers, leakage detection could be much more successful in the injection reservoir. We demonstrate proof-of-concept for this hypothesis, presenting a simulation where there is measurable pressure change at the injection well due to overpressurization, fault rupture, and consequent leakage up the fault into intermediate and shallow aquifers. The size of the detectible pressure change footprint is much larger in the reservoir than in either of the overlying aquifers. Further exploration of the range of conditions for which this technique would be successful is the topic of current study.« less

Trace metal concentrations in forest and lawn soils of Paris region (France) along a gradient of urban pressure

NASA Astrophysics Data System (ADS)

Ludovic, Foti

2017-04-01

Urban soils differ greatly from natural ones as they are located in areas of intense anthropogenic activity (e.g. pollution, physical disturbance, surface transformation). Urban soils are a crucial component of urban ecosystems, especially in public green spaces, and contribute to many ecosystem services from the mitigation of urban heat island to recreational services. In the last decade, the study of urban soils has emerged as an important frontier in environmental research, at least because of their impact on the quality of life of urban populations, because of the services they deliver and because they are more and more recognized as a valuable resource. One of the key issues is the pollution of urban soils because they receive a variety of deposits from local (vehicle emissions, industrial discharges, domestic heating, waste incineration and other anthropogenic activities) and from remote sources (through atmospheric transport). Typical contaminants include persistent toxic substances, such as trace metals (TMs) that have drawn wide attention due to their long persistence in the environment, their tendency to bioaccumulate in the food chain and their toxicity for humans and other organisms. Concentrations, spatial distributions, dynamics, impacts and sources of TMs (e.g. industry or fossil fuels combustion) have attracted a global interest in urban soils and are the subject of ongoing research (e.g. ecotoxicological urban ecology). Some studies have already documented soil pollution with TMs at both the town and regional scales. So far, several monitoring programs (e.g. National Network for the long term Monitoring of Forest Ecosystem, Regional Monitoring Quality of Soil in France) and studies have been carried out on a national scale to measure the ranges of TM concentrations and natural background values in French soils. These studies have focused on French agricultural and forest soils and have not tackled urban soils. No study has described TM concentrations and subsequent risks in soils of Paris and Paris region (Île-de-France). Our study aims at filling this knowledge gap, focusing on contamination and pollution by TMs in lawns and forests that constitute the main types of vegetation in urban areas of Paris region. Considering the rational described above, the aims of the present study were (i) to examine the concentration of eight selected TMs (As, Cd, Cr, Cu, Fe, Ni, Pb, Zn) in soils of two land-uses (public lawns and woods) along an urban pressure gradient in Paris region, (ii) to distinguish origins and sources of contamination or pollution, (iii) to evaluate the individual and overall TM contamination degree as well as the individual and overall TM pollution degree, (iiii) to use soil characteristics to better understand soil origins and histories along the urban pressure gradient and the relationship between these characteristics and TM concentrations. Ultimately, this study provides a baseline TM assessment for the long-term monitoring of the evolution of TM soil contents in urban area of the Paris region.

HYDRAULIC REDISTRIBUTION OF SOIL WATER: ECOSYSTEM IMPLICATIONS FOR PACIFIC NORTHWEST FORESTS

EPA Science Inventory

The physical process of hydraulic redistribution (HR) is driven by competing soil, tree and atmospheric water potential gradients, and may delay severe water stress for roots and other biota associated with the upper soil profile. We monitored soil moisture characteristics across...

Functional gene array-based analysis of microbial community structure in groundwaters with a gradient of contaminant levels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Waldron, P.J.; Wu, L.; Van Nostrand, J.D.

2009-06-15

To understand how contaminants affect microbial community diversity, heterogeneity, and functional structure, six groundwater monitoring wells from the Field Research Center of the U.S. Department of Energy Environmental Remediation Science Program (ERSP; Oak Ridge, TN), with a wide range of pH, nitrate, and heavy metal contamination were investigated. DNA from the groundwater community was analyzed with a functional gene array containing 2006 probes to detect genes involved in metal resistance, sulfate reduction, organic contaminant degradation, and carbon and nitrogen cycling. Microbial diversity decreased in relation to the contamination levels of the wells. Highly contaminated wells had lower gene diversity butmore » greater signal intensity than the pristine well. The microbial composition was heterogeneous, with 17-70% overlap between different wells. Metal-resistant and metal-reducing microorganisms were detected in both contaminated and pristine wells, suggesting the potential for successful bioremediation of metal-contaminated groundwaters. In addition, results of Mantel tests and canonical correspondence analysis indicate that nitrate, sulfate, pH, uranium, and technetium have a significant (p < 0.05) effect on microbial community structure. This study provides an overall picture of microbial community structure in contaminated environments with functional gene arrays by showing that diversity and heterogeneity can vary greatly in relation to contamination.« less

A high-performance liquid chromatography micromethod for the simultaneous determination of vigabatrin and gabapentin in serum.

PubMed

Ratnaraj, N; Patsalos, P N

1998-08-01

A gradient high-performance liquid chromatography micromethod is described for the simultaneous quantitation of vigabatrin and gabapentin in human serum. Chromatography was performed using a 125- x 3-mm ID Hypersil BDS C-18 column with a 3-microm mini-bore, eluted with a gradient system comprised of phosphate buffer (pH 6.5)-acetonitrile-methanol-water at a flow rate of 0.45 ml/minute. The column eluent was monitored on a fluorescence detector using excitation and emission wavelengths of 340 and 440 nm, respectively. The lower limit of quantitation for vigabatrin and for gabapentin was 5 micromol/l, and the within-batch and between-batch coefficients of variation were <5%. No interference from commonly prescribed antiepileptic drugs (carbamazepine and its metabolite carbamazepine epoxide, oxcarbazepine and its metabolite 10-hydroxycarbazepine, ethosuximide, lamotrigine, phenobarbitone, phenytoin, primidone, and valproic acid) was observed; thus, the method can be used to monitor vigabatrin and gabapentin in patients on polytherapy antiepileptic drug regimens.

Real-time Retrieving Atmospheric Parameters from Multi-GNSS Constellations

NASA Astrophysics Data System (ADS)

Li, X.; Zus, F.; Lu, C.; Dick, G.; Ge, M.; Wickert, J.; Schuh, H.

2016-12-01

The multi-constellation GNSS (e.g. GPS, GLONASS, Galileo, and BeiDou) bring great opportunities and challenges for real-time retrieval of atmospheric parameters for supporting numerical weather prediction (NWP) nowcasting or severe weather event monitoring. In this study, the observations from different GNSS are combined together for atmospheric parameter retrieving based on the real-time precise point positioning technique. The atmospheric parameters retrieved from multi-GNSS observations, including zenith total delay (ZTD), integrated water vapor (IWV), horizontal gradient (especially high-resolution gradient estimates) and slant total delay (STD), are carefully analyzed and evaluated by using the VLBI, radiosonde, water vapor radiometer and numerical weather model to independently validate the performance of individual GNSS and also demonstrate the benefits of multi-constellation GNSS for real-time atmospheric monitoring. Numerous results show that the multi-GNSS processing can provide real-time atmospheric products with higher accuracy, stronger reliability and better distribution, which would be beneficial for atmospheric sounding systems, especially for nowcasting of extreme weather.

Porous polycarbene-bearing membrane actuator for ultrasensitive weak-acid detection and real-time chemical reaction monitoring.

PubMed

Sun, Jian-Ke; Zhang, Weiyi; Guterman, Ryan; Lin, Hui-Juan; Yuan, Jiayin

2018-04-30

Soft actuators with integration of ultrasensitivity and capability of simultaneous interaction with multiple stimuli through an entire event ask for a high level of structure complexity, adaptability, and/or multi-responsiveness, which is a great challenge. Here, we develop a porous polycarbene-bearing membrane actuator built up from ionic complexation between a poly(ionic liquid) and trimesic acid (TA). The actuator features two concurrent structure gradients, i.e., an electrostatic complexation (EC) degree and a density distribution of a carbene-NH 3 adduct (CNA) along the membrane cross-section. The membrane actuator performs the highest sensitivity among the state-of-the-art soft proton actuators toward acetic acid at 10 -6  mol L -1 (M) level in aqueous media. Through competing actuation of the two gradients, it is capable of monitoring an entire process of proton-involved chemical reactions that comprise multiple stimuli and operational steps. The present achievement constitutes a significant step toward real-life application of soft actuators in chemical sensing and reaction technology.

Applications of MODIS satellite data and products for monitoring air quality in the state of Texas

NASA Astrophysics Data System (ADS)

Hutchison, Keith D.

The Center for Space Research (CSR), in conjunction with the Monitoring Operations Division (MOD) of the Texas Commission on Environmental Quality (TCEQ), is evaluating the use of remotely sensed satellite data to assist in monitoring and predicting air quality in Texas. The challenges of meeting air quality standards established by the US Environmental Protection Agency (US EPA) are impacted by the transport of pollution into Texas that originates from outside our borders and are cumulative with those generated by local sources. In an attempt to quantify the concentrations of all pollution sources, MOD has installed ground-based monitoring stations in rural regions along the Texas geographic boundaries including the Gulf coast, as well as urban regions that are the predominant sources of domestic pollution. However, analysis of time-lapse GOES satellite imagery at MOD, clearly demonstrates the shortcomings of using only ground-based observations for monitoring air quality across Texas. These shortcomings include the vastness of State borders, that can only be monitored with a large number of ground-based sensors, and gradients in pollution concentration that depend upon the location of the point source, the meteorology governing its transport to Texas, and its diffusion across the region. With the launch of NASA's MODerate resolution Imaging Spectroradiometer (MODIS), the transport of aerosol-borne pollutants can now be monitored over land and ocean surfaces. Thus, CSR and MOD personnel have applied MODIS data to several classes of pollution that routinely impact Texas air quality. Results demonstrate MODIS data and products can detect and track the migration of pollutants. This paper presents one case study in which continental haze from the northeast moved into the region and subsequently required health advisories to be issued for 150 counties in Texas. It is concluded that MODIS provides the basis for developing advanced data products that will, when used in conjunction with ground-based observations, create a cost-effective and accurate pollution monitoring system for the entire state of Texas.

Variation of socioeconomic gradients in children's developmental health across advanced Capitalist societies: analysis of 22 OECD nations.

PubMed

Siddiqi, Arjumand; Kawachi, Ichiro; Berkman, Lisa; Subramanian, S V; Hertzman, Clyde

2007-01-01

Within societies, there is a well-established relation between socioeconomic position and a wide range of outcomes related to well-being, and this relation is known to vary in magnitude across countries. Using a large sample of nations, the authors explored whether differences in social policies explain differences in socioeconomic gradients across nations. Analyses were conducted on reading literacy in 15-year-olds, as an outcome related to cognitive development and to a host of factors that contribute to future well-being, including educational attainment and health. The results show a systematic variation in socioeconomic gradients and average scores across countries. Scores were favorable in countries with a long history of welfare state regimes, but countries where institutional change unfolded more recently and rapidly, or where welfare states are less well developed, clustered at the bottom of the rankings. Strong support was found for the "flattening up" hypothesis, which suggests that nations with higher average scores have less socioeconomic inequality in scores (or flatter gradients). Potential explanations for the observed patterns include differences between nations in the extent and distribution of income and social goods important for children's development.

Shifts in Postdiscrimination Gradients within a Stimulus Dimension Based on Bilateral Facial Symmetry

ERIC Educational Resources Information Center

Derenne, Adam

2010-01-01

A shift in generalization gradients away from S+ and towards stimuli on the opposite end of the stimulus dimension from S- is a well established phenomenon in the laboratory, occurring with humans and nonhumans and with a wide range of stimuli. The phenomenon of gradient shifts has also been observed to have an analogous relationship to a variety…

Removal of microbial pathogens in a sandy gravel aquifer under forced-gradient subsurface flow conditions

NASA Astrophysics Data System (ADS)

Oudega, Thomas James; Derx, Julia; van Driezum, Inge; Cisneros, Anibal; Sommer, Regina; Kirschner, Alexander; Farnleitner, Andreas; Blaschke, Alfred Paul

2017-04-01

Subsurface media are being used around the world as a means to mitigate microbial contamination, but vary widely in their ability to remove pathogens. To help to provide accurate risk assessments of microbial contamination of groundwaters, and establish safe setback distances between receiving waters and disposal fields, this study aims to use aquifer tracer tests to evaluate the ability of subsurface media to attenuate these pathogens. The novelty of this work is the use of a variety of different tracer substances (e.g. phages, spores, microspheres, conservative tracers) together in field experiments. This will be done by means of injecting these substances under a forced gradient in a sandy gravel aquifer in Lobau, Austria. The extraction of the tracers will be monitored in a pumping well at a distrance of 50m downgradient. This will be able to provide us with insight to the characteristics of microbial transport and how the microorganisms react to the subsurface in the study site. Subsequent numerical modelling of the experiments can tell us more about quantification of subsurface processes such as attachment/detachment, inactivation and die-off of these substances. The first field experiment with conservative tracers (NaCl) has been carried out in December 2016, and subsequent tests are being planned for the next months.

Chloride interference in the determination of bromate in drinking water by reagent free ion chromatography with mass spectrometry detection.

PubMed

Cavalli, Silvano; Polesello, Stefano; Valsecchi, Sara

2005-08-26

Bromate, a well known by-product of the ozonation of drinking water, has been included among the substances which have to be monitored in the drinking water according to the last EC Directive 251/98 on potable water with a regulated limit of 10 microg l(-1). The need of performing routine analysis at this limit is a driving force for the developing of new simple and sensitive methods of detection, which should be also able to overcome the effect of matrix composition. This work explored the use of mass spectrometry detection with electrospray ionisation hyphenated to a reagent free ion chromatograph with hydroxide gradient elution for the determination of bromate in drinking water. The use of a high capacity hydroxide selective column operated in gradient mode allowed to avoid the interference by carbonate peak, which moved to longer retention times. The effect of increasing chloride concentrations from 0 to 250 mg l(-1), which is the guideline limit for drinking water in Directive 251/98/EC, was to decrease absolute mass spectrometric response and chromatographic efficiency and, on the consequence, to increase the effective detection limits. The effect of the chloride concentration on the detection of bromate is discussed.

Low-resolution ship detection from high-altitude aerial images

NASA Astrophysics Data System (ADS)

Qi, Shengxiang; Wu, Jianmin; Zhou, Qing; Kang, Minyang

2018-02-01

Ship detection from optical images taken by high-altitude aircrafts such as unmanned long-endurance airships and unmanned aerial vehicles has broad applications in marine fishery management, ship monitoring and vessel salvage. However, the major challenge is the limited capability of information processing on unmanned high-altitude platforms. Furthermore, in order to guarantee the wide detection range, unmanned aircrafts generally cruise at high altitudes, resulting in imagery with low-resolution targets and strong clutters suffered by heavy clouds. In this paper, we propose a low-resolution ship detection method to extract ships from these high-altitude optical images. Inspired by a recent research on visual saliency detection indicating that small salient signals could be well detected by a gradient enhancement operation combined with Gaussian smoothing, we propose the facet kernel filtering to rapidly suppress cluttered backgrounds and delineate candidate target regions from the sea surface. Then, the principal component analysis (PCA) is used to compute the orientation of the target axis, followed by a simplified histogram of oriented gradient (HOG) descriptor to characterize the ship shape property. Finally, support vector machine (SVM) is applied to discriminate real targets and false alarms. Experimental results show that the proposed method actually has high efficiency in low-resolution ship detection.

Cold hands, warm feet: sleep deprivation disrupts thermoregulation and its association with vigilance.

PubMed

Romeijn, Nico; Verweij, Ilse M; Koeleman, Anne; Mooij, Anne; Steimke, Rosa; Virkkala, Jussi; van der Werf, Ysbrand; Van Someren, Eus J W

2012-12-01

Vigilance is affected by induced and spontaneous skin temperature fluctuations. Whereas sleep deprivation strongly affects vigilance, no previous study examined in detail its effect on human skin temperature fluctuations and their association with vigilance. In a repeated-measures constant routine design, skin temperatures were assessed continuously from 14 locations while performance was assessed using a reaction time task, including eyes-open video monitoring, performed five times a day for 2 days, after a normal sleep or sleep deprivation night. Participants were seated in a dimly lit, temperature-controlled laboratory. Eight healthy young adults (five males, age 22.0 ± 1.8 yr (mean ± standard deviation)). One night of sleep deprivation. Mixed-effect regression models were used to evaluate the effect of sleep deprivation on skin temperature gradients of the upper (ear-mastoid), middle (hand-arm), and lower (foot-leg) body, and on the association between fluctuations in performance and in temperature gradients. Sleep deprivation induced a marked dissociation of thermoregulatory skin temperature gradients, indicative of attenuated heat loss from the hands co-occurring with enhanced heat loss from the feet. Sleep deprivation moreover attenuated the association between fluctuations in performance and temperature gradients; the association was best preserved for the upper body gradient. Sleep deprivation disrupts coordination of fluctuations in thermoregulatory skin temperature gradients. The dissociation of middle and lower body temperature gradients may therefore be evaluated as a marker for sleep debt, and the upper body gradient as a possible aid in vigilance assessment when sleep debt is unknown. Importantly, our findings suggest that sleep deprivation affects the coordination between skin blood flow fluctuations and the baroreceptor-mediated cardiovascular regulation that prevents venous pooling of blood in the lower limbs when there is the orthostatic challenge of an upright posture.

Noise reduction in the intracellular pom1p gradient by a dynamic clustering mechanism.

PubMed

Saunders, Timothy E; Pan, Kally Z; Angel, Andrew; Guan, Yinghua; Shah, Jagesh V; Howard, Martin; Chang, Fred

2012-03-13

Chemical gradients can generate pattern formation in biological systems. In the fission yeast Schizosaccharomyces pombe, a cortical gradient of pom1p (a DYRK-type protein kinase) functions to position sites of cytokinesis and cell polarity and to control cell length. Here, using quantitative imaging, fluorescence correlation spectroscopy, and mathematical modeling, we study how its gradient distribution is formed. Pom1p gradients exhibit large cell-to-cell variability, as well as dynamic fluctuations in each individual gradient. Our data lead to a two-state model for gradient formation in which pom1p molecules associate with the plasma membrane at cell tips and then diffuse on the membrane while aggregating into and fragmenting from clusters, before disassociating from the membrane. In contrast to a classical one-component gradient, this two-state gradient buffers against cell-to-cell variations in protein concentration. This buffering mechanism, together with time averaging to reduce intrinsic noise, allows the pom1p gradient to specify positional information in a robust manner. Copyright © 2012 Elsevier Inc. All rights reserved.

Evolutionary responses of tree phenology to the combined effects of assortative mating, gene flow and divergent selection

PubMed Central

Soularue, J-P; Kremer, A

2014-01-01

The timing of bud burst (TBB) in temperate trees is a key adaptive trait, the expression of which is triggered by temperature gradients across the landscape. TBB is strongly correlated with flowering time and is therefore probably mediated by assortative mating. We derived theoretical predictions and realized numerical simulations of evolutionary changes in TBB in response to divergent selection and gene flow in a metapopulation. We showed that the combination of the environmental gradient of TBB and assortative mating creates contrasting genetic clines, depending on the direction of divergent selection. If divergent selection acts in the same direction as the environmental gradient (cogradient settings), genetic clines are established and inflated by assortative mating. Conversely, under divergent selection of the same strength but acting in the opposite direction (countergradient selection), genetic clines are slightly constrained. We explored the consequences of these dynamics for population maladaptation, by monitoring pollen swamping. Depending on the direction of divergent selection with respect to the environmental gradient, pollen filtering owing to assortative mating either facilitates or impedes adaptation in peripheral populations. PMID:24924591

Chemotaxis of Amoeba proteus in the developing pH gradient within a pocket-like chamber studied with the computer assisted method.

PubMed

Korohoda, W; Golda, J; Sroka, J; Wojnarowicz, A; Jochym, P; Madeja, Z

1997-01-01

A new "U" shaped, pocket-like chamber was used to observe the chemotactic responses of individual cells. This method permits monitoring of both the development of the concentration gradient of a tested substance and cell locomotion. We investigated the chemotactic responses of Amoeba proteus and observed that the amoebae moved in positively and negatively developing [H+] gradients towards the solution of lower pH in a pH range 5.75-7.75. The chemotactic response of amoebae to [H+] gradients required the presence of extracellular calcium ions. It was blocked and random locomotion was restored by the replacement of calcium with magnesium in the cell medium. Time-lapse video recording and data processing were accomplished with computer-assisted methods. This made it possible to compare selected methods of data presentation and analysis for cells locomoting in isotropic and anisotropic conditions. The cell trajectories were determined and displayed in circular diagrams, lengths of cell tracks and final cell displacements were estimated and a few parameters characterizing cell locomotion were computed.

A fast referenceless PRFS-based MR thermometry by phase finite difference

NASA Astrophysics Data System (ADS)

Zou, Chao; Shen, Huan; He, Mengyue; Tie, Changjun; Chung, Yiu-Cho; Liu, Xin

2013-08-01

Proton resonance frequency shift-based MR thermometry is a promising temperature monitoring approach for thermotherapy but its accuracy is vulnerable to inter-scan motion. Model-based referenceless thermometry has been proposed to address this problem but phase unwrapping is usually needed before the model fitting process. In this paper, a referenceless MR thermometry method using phase finite difference that avoids the time consuming phase unwrapping procedure is proposed. Unlike the previously proposed phase gradient technique, the use of finite difference in the new method reduces the fitting error resulting from the ringing artifacts associated with phase discontinuity in the calculation of the phase gradient image. The new method takes into account the values at the perimeter of the region of interest because of their direct relevance to the extrapolated baseline phase of the region of interest (where temperature increase takes place). In simulation study, in vivo and ex vivo experiments, the new method has a root-mean-square temperature error of 0.35 °C, 1.02 °C and 1.73 °C compared to 0.83 °C, 2.81 °C, and 3.76 °C from the phase gradient method, respectively. The method also demonstrated a slightly higher, albeit small, temperature accuracy than the original referenceless MR thermometry method. The proposed method is computationally efficient (∼0.1 s per image), making it very suitable for the real time temperature monitoring.

Changes in intracranial pressure gradients between the cerebral hemispheres in patients with intracerebral hematomas in one cerebral hemisphere.

PubMed

Qiu, Wusi; Jiang, Qizhou; Xiao, Guoming; Wang, Weiming; Shen, Hong

2014-01-01

Intracranial-pressure (ICP) monitoring is useful for patients with increased ICP following hemorrhagic stroke. In this study, the changes in pressure gradients between the two cerebral hemispheres were investigated after hemorrhagic stroke of one side, and after a craniotomy. Twenty-four patients with acute cerebral hemorrhages and intracerebral hematomas who exhibited mass effect and midline shift to the contralateral side on computed tomography were selected for this study. After admission, both sides of the cranium were drilled, and optical fiber sensors were implanted to monitor the brain parenchyma pressure (BPP) in both cerebral hemispheres. All patients underwent surgical hematoma evacuations. The preoperative and postoperative BPP data from both cerebral hemispheres were collected at various time points and compared pairwise. There were statistically significant differences (P < 0.01) in the preoperative BPP values between the two hemispheres at three different time points. Differences in the BPP values between the two hemispheres at the time of surgery, and 24 and 48 h after surgery, were not statistically significant (P > 0.05). The posteroperative BPPs of both hemispheres were statistically significantly lower than preoperative recordings. BPP sensors should be applied to the injured cerebral hemisphere, because this becomes the source of increased ICP. Hematoma evacuation surgery effectively decreases ICP and eliminates pressure gradients between the two cerebral hemispheres, consequently enabling brain shift correction.

Multilevel groundwater monitoring of hydraulic head and temperature in the eastern Snake River Plain aquifer, Idaho National Laboratory, Idaho, 2007-08

USGS Publications Warehouse

Fisher, Jason C.; Twining, Brian V.

2011-01-01

During 2007 and 2008, the U.S. Geological Survey, in cooperation with the U.S. Department of Energy, collected quarterly depth-discrete measurements of fluid pressure and temperature in six boreholes located in the eastern Snake River Plain aquifer of Idaho. Each borehole was instrumented with a multilevel monitoring system consisting of a series of valved measurement ports, packer bladders, casing segments, and couplers. Hydraulic heads (head) and water temperatures in boreholes were monitored at 86 hydraulically-isolated depth intervals located 448.0 to 1,377.6 feet below land surface. The calculation of head is most sensitive to fluid pressure and the altitude of the pressure transducer at each port coupling; it is least sensitive to barometric pressure and water temperature. An analysis of errors associated with the head calculation determined the accuracy of an individual head measurement at +/- 2.3 feet. Many of the sources of measurement error are diminished when considering the differences between two closely-spaced readings of head; therefore, a +/- 0.1 foot measurement accuracy was assumed for vertical head differences (and gradients) calculated between adjacent monitoring zones. Vertical head and temperature profiles were unique to each borehole, and were characteristic of the heterogeneity and anisotropy of the eastern Snake River Plain aquifer. The vertical hydraulic gradients in each borehole remained relatively constant over time with minimum Pearson correlation coefficients between head profiles ranging from 0.72 at borehole USGS 103 to 1.00 at boreholes USGS 133 and MIDDLE 2051. Major inflections in the head profiles almost always coincided with low permeability sediment layers. The presence of a sediment layer, however, was insufficient for identifying the location of a major head change in a borehole. The vertical hydraulic gradients were defined for the major inflections in the head profiles and were as much as 2.2 feet per foot. Head gradients generally were downward in boreholes USGS 133, 134, and MIDDLE 2050A, zero in boreholes USGS 103 and 132, and exhibited a reversal in direction in borehole MIDDLE 2051. Water temperatures in all boreholes ranged from 10.2 to 16.3 degrees Celsius. Boreholes USGS 103 and 132 are in an area of concentrated volcanic vents and fissures, and measurements show water temperature decreasing with depth. All other measurements in boreholes show water temperature increasing with depth. A comparison among boreholes of the normalized mean head over time indicates a moderately positive correlation.

The Potential of Tropospheric Gradients for Regional Precipitation Prediction

NASA Astrophysics Data System (ADS)

Boisits, Janina; Möller, Gregor; Wittmann, Christoph; Weber, Robert

2017-04-01

Changes of temperature and humidity in the neutral atmosphere cause variations in tropospheric path delays and tropospheric gradients. By estimating zenith wet delays (ZWD) and gradients using a GNSS reference station network the obtained time series provide information about spatial and temporal variations of water vapour in the atmosphere. Thus, GNSS-based tropospheric parameters can contribute to the forecast of regional precipitation events. In a recently finalized master thesis at TU Wien the potential of tropospheric gradients for weather prediction was investigated. Therefore, ZWD and gradient time series at selected GNSS reference stations were compared to precipitation data over a period of six months (April to September 2014). The selected GNSS stations form two test areas within Austria. All required meteorological data was provided by the Central Institution for Meteorology and Geodynamics (ZAMG). Two characteristics in ZWD and gradient time series can be anticipated in case of an approaching weather front. First, an induced asymmetry in tropospheric delays results in both, an increased magnitude of the gradient and in gradients pointing towards the weather front. Second, an increase in ZWD reflects the increased water vapour concentration right before a precipitation event. To investigate these characteristics exemplary test events were processed. On the one hand, the sequence of the anticipated increase in ZWD at each GNSS station obtained by cross correlation of the time series indicates the direction of the approaching weather front. On the other hand, the corresponding peak in gradient time series allows the deduction of the direction of movement as well. To verify the results precipitation data from ZAMG was used. It can be deduced, that tropospheric gradients show high potential for predicting precipitation events. While ZWD time series rather indicate the orientation of the air mass boundary, gradients rather indicate the direction of movement of an approaching weather front. Additionally our investigations have shown that gradients are able to capture the characteristics of an approaching weather front twenty to thirty hours before the precipitation event, which allows a first indication well in advance. Thus in conclusion, the utilization of GNSS tropospheric parameters, in particular tropospheric gradients, has the potential to contribute substantially to weather forecasting models.

Diabetes induced renal urea transport alterations assessed with 3D hyperpolarized 13 C,15 N-Urea.

PubMed

Bertelsen, Lotte B; Nielsen, Per M; Qi, Haiyun; Nørlinger, Thomas S; Zhang, Xiaolu; Stødkilde-Jørgensen, Hans; Laustsen, Christoffer

2017-04-01

In the current study, we investigated hyperpolarized urea as a possible imaging biomarker of the renal function by means of the intrarenal osmolality gradient. Hyperpolarized three-dimensional balanced steady state 13 C MRI experiments alongside kidney function parameters and quantitative polymerase chain reaction measurements was performed on two groups of rats, a streptozotocin type 1 diabetic group and a healthy control group. A significant decline in intrarenal steepness of the urea gradient was found after 4 weeks of untreated insulinopenic diabetes in agreement with an increased urea transport transcription. MRI and hyperpolarized [ 13 C, 15 N]urea can monitor the changes in the corticomedullary urea concentration gradients in diabetic and healthy control rats. Magn Reson Med 77:1650-1655, 2017. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

Ecophysiological Changes in Microbial Mats Incubated in a Greenhouse Collaboratory

NASA Technical Reports Server (NTRS)

Bebout, Brad; DesMarais, David J.; GarciaPichel, Ferran; Hogan, Mary; Jahnke, Linda; Keller, Richard M.; Miller, Scott R.

2001-01-01

Microbial mats are modern examples of the earliest microbial communities known. Among the best studied are microbial mats growing in hypersaline ponds managed for the production of salt by Exportadora de Sal, S.A. de C.V., Guerrero Negro, Baja California Sur, Mexico. In May, 2001, we collected mats from Ponds 4 and 5 in this system and returned them to Ames Research Center, where they have been maintained for a period of over nine months. We report here on both the ecophysiological changes occurring in the mats over that period of time as well as the facility in which they were incubated. Mats (approximately 1 sq. meter total area) were incubated in a greenhouse facility modified to provide the mats with natural levels of visible and ultraviolet radiation as well as constantly flowing, temperature-controlled water. Two replicated treatments were maintained, a 'high salinity' treatment (about 120 ppt) and a 'low salinity' treatment (about 90 ppt). Rates of net biological activity (e.g., photosynthesis, respiration, trace gas production) in the mats were relatively constant over the several months, and were similar to rates of activity measured in the field. However, over the course of the incubation, mats in both treatments changed in physical appearance. The most obvious change was that mats in the higher salinity treatments developed a higher proportion of carotenoid pigments (relative to chlorophyll), resulting in a noticeably orange color in the high salinity mats. This trend is also seen in the natural salinity gradient present at the field site. Changes in the community composition of the mats, as assayed by denaturing gradient gel electrophoresis (DGGE), as well as biomarker compounds produced in the mats were also monitored. The degree to which the mats kept in the greenhouse changed from the originally collected mats, as well as differences between high and low salinity mats will be discussed. Additional information is contained in the original extended abstract.

Three-year observations of halocarbons at the Nepal Climate Observatory at Pyramid (NCO-P, 5079 m a.s.l.) on the Himalayan range

NASA Astrophysics Data System (ADS)

Maione, M.; Giostra, U.; Arduini, J.; Furlani, F.; Bonasoni, P.; Cristofanelli, P.; Laj, P.; Vuillermoz, E.

2011-04-01

A monitoring programme for halogenated climate-altering gases has been established in the frame of the SHARE EV-K2-CNR project at the Nepal Climate Laboratory - Pyramid in the Himalayan range at the altitude of 5079 m a.s.l. The site is very well located to provide important insights on changes in atmospheric composition in a region that is of great significance for emissions of both anthropogenic and biogenic halogenated compounds. Measurements are performed since March 2006, with grab samples collected on a weekly basis. The first three years of data have been analysed. After the identification of the atmospheric background values for fourteen halocarbons, the frequency of occurrence of pollution events have been compared with the same kind of analysis for data collected at other global background stations. The analysis showed the fully halogenated species, whose production and consumption are regulated under the Montreal Protocol, show a significant occurrence of "above the baseline" values, as a consequence of their current use in the developing countries surrounding the region, meanwhile the hydrogenated gases, more recently introduced into the market, show less frequent spikes. Atmospheric concentration trends have been calculated as well, and they showed a fast increase, ranging from 5.7 to 12.6%, of all the hydrogenated species, and a clear decrease of methyl chloroform (-17.7%). The comparison with time series from other stations has also allowed to derive Meridional gradients, which are absent for long living well mixed species, while for the more reactive species, the gradient increases inversely with respect to their atmospheric lifetime. The effect of long range transport and of local events on the atmospheric composition at the station has been analysed as well, allowing the identification of relevant source regions the Northern half of the Indian sub-continent. Also, at finer spatial scales, a smaller, local contribution of forest fires from the Khumbu valley has been detected.

Experimental high gradient testing of a 17.1 GHz photonic band-gap accelerator structure

DOE PAGES

Munroe, Brian J.; Zhang, JieXi; Xu, Haoran; ...

2016-03-29

In this paper, we report the design, fabrication, and high gradient testing of a 17.1 GHz photonic band-gap (PBG) accelerator structure. Photonic band-gap (PBG) structures are promising candidates for electron accelerators capable of high-gradient operation because they have the inherent damping of high order modes required to avoid beam breakup instabilities. The 17.1 GHz PBG structure tested was a single cell structure composed of a triangular array of round copper rods of radius 1.45 mm spaced by 8.05 mm. The test assembly consisted of the test PBG cell located between conventional (pillbox) input and output cells, with input power ofmore » up to 4 MW from a klystron supplied via a TM 01 mode launcher. Breakdown at high gradient was observed by diagnostics including reflected power, downstream and upstream current monitors and visible light emission. The testing procedure was first benchmarked with a conventional disc-loaded waveguide structure, which reached a gradient of 87 MV=m at a breakdown probability of 1.19 × 10 –1 per pulse per meter. The PBG structure was tested with 100 ns pulses at gradient levels of less than 90 MV=m in order to limit the surface temperature rise to 120 K. The PBG structure reached up to 89 MV=m at a breakdown probability of 1.09 × 10 –1 per pulse per meter. These test results show that a PBG structure can simultaneously operate at high gradients and low breakdown probability, while also providing wakefield damping.« less

Sensors for Metering Heat Flux Area Density and Metrological Equipment for the Heat Flux Density Measurement

NASA Astrophysics Data System (ADS)

Doronin, D. O.

2018-04-01

The demand in measuring and studies of heat conduction of various media is very urgent now. This article considers the problem of heat conduction monitoring and measurement in various media and materials in any industries and branches of science as well as metrological support of the heat flux measurement equipment. The main study objects are both the sensors manufactured and facilities onto which these sensors will be installed: different cladding structures of the buildings, awnings, rocket fairings, boiler units, internal combustion engines. The Company develops and manufactures different types of heat flux sensors: thermocouple, thin-film, heterogeneous gradient as well as metrological equipment for the gauging calibration of the heat flux density measurement. The calibration shall be performed using both referencing method in the unit and by fixed setting of the heat flux in the unit. To manufacture heterogeneous heat flux gradient sensors (HHFGS) the Company developed and designed a number of units: diffusion welding unit, HHFGS cutting unit. Rather good quality HHFGS prototypes were obtained. At this stage the factory tests on the equipment for the heat flux density measurement equipment are planned. A high-sensitivity heat flux sensor was produced, now it is tested at the Construction Physics Research Institute (Moscow). It became possible to create thin-film heat flux sensors with the sensitivity not worse than that of the sensors manufactured by Captec Company (France). The Company has sufficient premises to supply the market with a wide range of sensors, to master new sensor manufacture technologies which will enable their application range.

Crustal wavespeed structure of North Texas and Oklahoma based on ambient noise cross-correlation functions and adjoint tomography

NASA Astrophysics Data System (ADS)

Zhu, Hejun

2018-04-01

Recently, seismologists observed increasing seismicity in North Texas and Oklahoma. Based on seismic observations and other geophysical measurements, numerous studies suggested links between the increasing seismicity and wastewater injection during unconventional oil and gas exploration. To better monitor seismic events and investigate their triggering mechanisms, we need an accurate 3D crustal wavespeed model for the study region. Considering the uneven distribution of earthquakes in this area, seismic tomography with local earthquake records have difficulties achieving even illumination. To overcome this limitation, in this study, ambient noise cross-correlation functions are used to constrain subsurface variations in wavespeeds. I use adjoint tomography to iteratively fit frequency-dependent phase differences between observed and predicted band-limited Green's functions. The spectral-element method is used to numerically calculate the band-limited Green's functions and the adjoint method is used to calculate misfit gradients with respect to wavespeeds. Twenty five preconditioned conjugate gradient iterations are used to update model parameters and minimize data misfits. Features in the new crustal model TO25 correlates well with geological provinces in the study region, including the Llano uplift, the Anadarko basin and the Ouachita orogenic front, etc. In addition, there are relatively good correlations between seismic results with gravity and magnetic observations. This new crustal model can be used to better constrain earthquake source parameters in North Texas and Oklahoma, such as epicenter location as well as moment tensor solutions, which are important for investigating triggering mechanisms between these induced earthquakes and unconventional oil and gas exploration activities.

Method and apparatus for fabrication of high gradient insulators with parallel surface conductors spaced less than one millimeter apart

DOEpatents

Sanders, David M.; Decker, Derek E.

1999-01-01

Optical patterns and lithographic techniques are used as part of a process to embed parallel and evenly spaced conductors in the non-planar surfaces of an insulator to produce high gradient insulators. The approach extends the size that high gradient insulating structures can be fabricated as well as improves the performance of those insulators by reducing the scale of the alternating parallel lines of insulator and conductor along the surface. This fabrication approach also substantially decreases the cost required to produce high gradient insulators.

Fate of herbicides in a shallow aerobic aquifer: A continuous field injection experiment (Vejen, Denmark)

NASA Astrophysics Data System (ADS)

Broholm, Mette M.; Rügge, Kirsten; Tuxen, Nina; HøJberg, Anker L.; MosbæK, Hans; Bjerg, Poul L.

2001-12-01

A continuous, natural gradient, field injection experiment, involving six herbicides and a tracer, was performed in a shallow aerobic aquifer near Vejen, Denmark. Bentazone, (±)-2-(4-chloro-2-methylphenoxy) propanoic acid (MCPP), dichlorprop, isoproturon, and the dichlobenil metabolite 2,6-dichlor-benzamide (BAM) were injected along with 2-methyl-4,6-dinitrophenol (not discussed in this paper) and the tracer bromide. The injection lasted for 216 days and created a continuous plume in the aquifer. The plume was monitored in three dimensions in 96 multilevel samplers of 6-9 points each for 230 days, with selected individual points for a longer period. The bromide plume followed a complex path through the monitoring network downgradient of the injection wells. The plume movement was controlled by spatially varied hydraulic conductivities of the sand deposit and influenced by asynchronous seasonal variation in groundwater potentials. An average flow velocity of 0.5 m/d was observed, as depicted by bromide. Bentazone, BAM, MCPP, and dichlorprop retardation was negligible, and only slight retardation of isoproturon was observed in the continuous injection experiment and a preceding pulse experiment. No degradation of bentazone was observed in the aerobic aquifer during the monitoring period. BAM and isoproturon were not degraded within 5 m downgradient of the injection. The two phenoxy acids MCPP and dichlorprop were both degraded in the aerobic aquifer. Near the source a lag phase was observed followed by fast degradation of the phenoxy acids, indicating growth kinetics. The phenoxy acids were completely degraded within l m downgradient of the injection wells, resulting in the plumes being divided into small plumes at the injection wells and pulses farther downgradient. During the lag phase, phenoxy acids had spread beyond the 25 m long monitoring network. However, the mass of the phenoxy acids passing the 10-25 m fences never matched the corresponding bentazone or bromide masses, and the pulse was observed to shrink in size. This indicates that this pulse of phenoxy acids was being partially degraded at a low rate as it traveled through the aquifer.

Cultivation of mammalian cells using a single-use pneumatic bioreactor system.

PubMed

Obom, Kristina M; Cummings, Patrick J; Ciafardoni, Janelle A; Hashimura, Yasunori; Giroux, Daniel

2014-10-10

Recent advances in mammalian, insect, and stem cell cultivation and scale-up have created tremendous opportunities for new therapeutics and personalized medicine innovations. However, translating these advances into therapeutic applications will require in vitro systems that allow for robust, flexible, and cost effective bioreactor systems. There are several bioreactor systems currently utilized in research and commercial settings; however, many of these systems are not optimal for establishing, expanding, and monitoring the growth of different cell types. The culture parameters most challenging to control in these systems include, minimizing hydrodynamic shear, preventing nutrient gradient formation, establishing uniform culture medium aeration, preventing microbial contamination, and monitoring and adjusting culture conditions in real-time. Using a pneumatic single-use bioreactor system, we demonstrate the assembly and operation of this novel bioreactor for mammalian cells grown on micro-carriers. This bioreactor system eliminates many of the challenges associated with currently available systems by minimizing hydrodynamic shear and nutrient gradient formation, and allowing for uniform culture medium aeration. Moreover, the bioreactor's software allows for remote real-time monitoring and adjusting of the bioreactor run parameters. This bioreactor system also has tremendous potential for scale-up of adherent and suspension mammalian cells for production of a variety therapeutic proteins, monoclonal antibodies, stem cells, biosimilars, and vaccines.

Assessing cadmium and vanadium accumulation using diffusive gradient in thin-films (DGT) and phytoplankton in the Churchill River estuary, Manitoba.

PubMed

Mangal, V; Zhu, Y; Shi, Y X; Guéguen, C

2016-11-01

Diffusive gradient in thin films (DGT) and phytoplankton communities were evaluated for the measurement of Cd and V at environmentally relevant concentrations in laboratory settings and in the Churchill River estuary (Manitoba, Canada) during an annual spring melt. Despite rapid changes in hydrology and water quality, DGT samplers and intracellular Cd and V concentrations were positively correlated (0.79 < r(2) < 0.99), suggesting comparable accumulation trends between both DGT-labile and intracellular monitoring techniques. The largest accumulated concentrations of both Cd and V by DGT and phytoplankton accumulation methods were found later into the river discharge period. In controlled settings, accumulated Cd and V concentrations by the diatom Attheya septentrionalis displayed a strong correlation with metals accumulated by DGTs (r(2) > 0.99). Principal component analysis (PCA) reinforced similarities between both metal monitoring techniques and assessed how changing environmental variables during the river discharge period influenced each monitoring technique. Cd accumulation was influenced by DOC concentrations and protein-like DOM whereas ionic strength (i.e. conductivity) and humic-like DOM influenced V accumulation. The present findings suggest that (1) DGT is a versatile tool for monitoring bioaccumulation of Cd and V in highly dynamic environmental systems and (2) DOC concentration, DOM composition, conductivity, pH, and river discharge influence the bioavailability of Cd and V in estuarine and riverine waters. Copyright © 2016 Elsevier Ltd. All rights reserved.

Calibration of a rotating accelerometer gravity gradiometer using centrifugal gradients

NASA Astrophysics Data System (ADS)

Yu, Mingbiao; Cai, Tijing

2018-05-01

The purpose of this study is to calibrate scale factors and equivalent zero biases of a rotating accelerometer gravity gradiometer (RAGG). We calibrate scale factors by determining the relationship between the centrifugal gradient excitation and RAGG response. Compared with calibration by changing the gravitational gradient excitation, this method does not need test masses and is easier to implement. The equivalent zero biases are superpositions of self-gradients and the intrinsic zero biases of the RAGG. A self-gradient is the gravitational gradient produced by surrounding masses, and it correlates well with the RAGG attitude angle. We propose a self-gradient model that includes self-gradients and the intrinsic zero biases of the RAGG. The self-gradient model is a function of the RAGG attitude, and it includes parameters related to surrounding masses. The calibration of equivalent zero biases determines the parameters of the self-gradient model. We provide detailed procedures and mathematical formulations for calibrating scale factors and parameters in the self-gradient model. A RAGG physical simulation system substitutes for the actual RAGG in the calibration and validation experiments. Four point masses simulate four types of surrounding masses producing self-gradients. Validation experiments show that the self-gradients predicted by the self-gradient model are consistent with those from the outputs of the RAGG physical simulation system, suggesting that the presented calibration method is valid.

Method for simulating paint mixing on computer monitors

NASA Astrophysics Data System (ADS)

Carabott, Ferdinand; Lewis, Garth; Piehl, Simon

2002-06-01

Computer programs like Adobe Photoshop can generate a mixture of two 'computer' colors by using the Gradient control. However, the resulting colors diverge from the equivalent paint mixtures in both hue and value. This study examines why programs like Photoshop are unable to simulate paint or pigment mixtures, and offers a solution using Photoshops existing tools. The article discusses how a library of colors, simulating paint mixtures, is created from 13 artists' colors. The mixtures can be imported into Photoshop as a color swatch palette of 1248 colors and as 78 continuous or stepped gradient files, all accessed in a new software package, Chromafile.

Combustor deployments of femtosecond laser written fiber Bragg grating arrays for temperature measurements surpassing 1000°C

NASA Astrophysics Data System (ADS)

Walker, Robert B.; Ding, Huimin; Coulas, David; Mihailov, Stephen J.; Duchesne, Marc A.; Hughes, Robin W.; McCalden, David J.; Burchat, Ryan; Yandon, Robert; Yun, Sangsig; Ramachandran, Nanthan; Charbonneau, Michel

2017-05-01

Femtosecond Infrared (fs-IR) laser written fiber Bragg gratings (FBGs), have demonstrated great potential for extreme sensing. Such conditions are inherent to advanced power plant technologies and gas turbine engines, under development to reduce greenhouse gas emissions; and the ability to measure temperature gradients in these harsh environments is currently limited by the lack of sensors and controls capable of withstanding the high temperature, pressure and corrosive conditions present. This paper reviews our fabrication and deployment of hundreds of fs-IR written FBGs, for monitoring temperature gradients of an oxy-fuel fluidized bed combustor and an aerospace gas turbine combustor simulator.

The Educational Gradient in Health in China

PubMed Central

Chen, Qiulin; Eggleston, Karen; Zhang, Wei; Zhao, Jiaying; Zhou, Sen

2017-01-01

It has been well established that better educated individuals enjoy better health and longevity. In theory, the educational gradients in health could be flattening if diminishing returns to improved average education levels and the influence of earlier population health interventions outweigh the gradient-steepening effects of new medical and health technologies. This paper documents how the gradients are evolving in China, a rapidly developing country, about which little is known on this topic. Based on recent mortality data and nationally representative health surveys, we find large and, in some cases, steepening educational gradients. We also find that the gradients vary by cohort, gender and region. Further, we find that the gradients can only partially be accounted for by economic factors. These patterns highlight the double disadvantage of those with low education, and suggest the importance of policy interventions that foster both aspects of human capital for them. PMID:29056815

Numerical optimization in Hilbert space using inexact function and gradient evaluations

NASA Technical Reports Server (NTRS)

Carter, Richard G.

1989-01-01

Trust region algorithms provide a robust iterative technique for solving non-convex unstrained optimization problems, but in many instances it is prohibitively expensive to compute high accuracy function and gradient values for the method. Of particular interest are inverse and parameter estimation problems, since function and gradient evaluations involve numerically solving large systems of differential equations. A global convergence theory is presented for trust region algorithms in which neither function nor gradient values are known exactly. The theory is formulated in a Hilbert space setting so that it can be applied to variational problems as well as the finite dimensional problems normally seen in trust region literature. The conditions concerning allowable error are remarkably relaxed: relative errors in the gradient error condition is automatically satisfied if the error is orthogonal to the gradient approximation. A technique for estimating gradient error and improving the approximation is also presented.

Structural optimization with approximate sensitivities

NASA Technical Reports Server (NTRS)

Patnaik, S. N.; Hopkins, D. A.; Coroneos, R.

1994-01-01

Computational efficiency in structural optimization can be enhanced if the intensive computations associated with the calculation of the sensitivities, that is, gradients of the behavior constraints, are reduced. Approximation to gradients of the behavior constraints that can be generated with small amount of numerical calculations is proposed. Structural optimization with these approximate sensitivities produced correct optimum solution. Approximate gradients performed well for different nonlinear programming methods, such as the sequence of unconstrained minimization technique, method of feasible directions, sequence of quadratic programming, and sequence of linear programming. Structural optimization with approximate gradients can reduce by one third the CPU time that would otherwise be required to solve the problem with explicit closed-form gradients. The proposed gradient approximation shows potential to reduce intensive computation that has been associated with traditional structural optimization.

Regional gravity field modelling from GOCE observables

NASA Astrophysics Data System (ADS)

Pitoňák, Martin; Šprlák, Michal; Novák, Pavel; Tenzer, Robert

2017-01-01

In this article we discuss a regional recovery of gravity disturbances at the mean geocentric sphere approximating the Earth over the area of Central Europe from satellite gravitational gradients. For this purpose, we derive integral formulas which allow converting the gravity disturbances onto the disturbing gravitational gradients in the local north-oriented frame (LNOF). The derived formulas are free of singularities in case of r ≠ R . We then investigate three numerical approaches for solving their inverses. In the initial approach, the integral formulas are firstly modified for solving individually the near- and distant-zone contributions. While the effect of the near-zone gravitational gradients is solved as an inverse problem, the effect of the distant-zone gravitational gradients is computed by numerical integration from the global gravitational model (GGM) TIM-r4. In the second approach, we further elaborate the first scenario by reducing measured gravitational gradients for gravitational effects of topographic masses. In the third approach, we apply additional modification by reducing gravitational gradients for the reference GGM. In all approaches we determine the gravity disturbances from each of the four accurately measured gravitational gradients separately as well as from their combination. Our regional gravitational field solutions are based on the GOCE EGG_TRF_2 gravitational gradients collected within the period from November 1 2009 until January 11 2010. Obtained results are compared with EGM2008, DIR-r1, TIM-r1 and SPW-r1. The best fit, in terms of RMS (2.9 mGal), is achieved for EGM2008 while using the third approach which combine all four well-measured gravitational gradients. This is explained by the fact that a-priori information about the Earth's gravitational field up to the degree and order 180 was used.

On the impact of adverse pressure gradient on the supersonic turbulent boundary layer

NASA Astrophysics Data System (ADS)

Wang, Qian-Cheng; Wang, Zhen-Guo; Zhao, Yu-Xin

2016-11-01

By employing the particle image velocimetry, the mean and turbulent characteristics of a Mach 2.95 turbulent boundary layer are experimentally investigated without the impact of curvature. The physical mechanism with which the streamwise adverse pressure gradient affects the supersonic boundary layer is revealed. The data are compared to that of the concave boundary layer with similar streamwise distributions of wall static pressure to clarify the separate impacts of the adverse pressure gradient and the concave curvature. The logarithmic law is observed to be well preserved for both of the cases. The dip below the logarithmic law is not observed in present investigation. Theoretical analysis indicates that it could be the result of compromise between the opposite impacts of the compression wave and the increased turbulent intensity. Compared to the zero pressure gradient boundary layer, the principal strain rate and the turbulent intensities are increased by the adverse pressure gradient. The shear layer formed due the hairpin packets could be sharpened by the compression wave, which leads to higher principal strain rate and the associated turbulent level. Due to the additional impact of the centrifugal instability brought by the concave wall, even higher turbulent intensities than that of the adverse pressure gradient case are introduced. The existence of velocity modes within the zero pressure gradient boundary layer suggests that the large scale motions are statistically well organized. The generation of new velocity modes due to the adverse pressure gradient indicates that the turbulent structure is changed by the adverse pressure gradient, through which more turbulence production that cannot be effectively predicted by the Reynolds-stress transport equations could be brought.

On the feasibility to conduct gradient liquid chromatography separations in narrow-bore columns at pressures up to 2000bar.

PubMed

De Pauw, Ruben; Swier, Tim; Degreef, Bart; Desmet, Gert; Broeckhoven, Ken

2016-11-18

The limits in operating pressures are extended for narrow-bore columns in gradient elution up to 2000bar. As the required pumps for these pressures are incompatible with common chromatographic solvents and are not suitable to apply a mobile phase composition gradient, a mobile phase delivery and injection system is described and experimentally validated which allows to use any possible chromatographic solvent in isocratic and gradient elution. The mobile phase delivery and injection system also allows to perform multiple separations without the need to depressurize the column. This system consists out of 5 dual on/off valves and two large volume loops in which the gradient and equilibration volume of initial mobile phase are loaded by a commercial liquid chromatography pump. The loops are then flushed toward the column at extreme pressures. The mobile phase delivery and injection system is first evaluated in isocratic elution and shows a comparable performance to a state-of-the-art commercial flow-through-needle injector but with twice the pressure rating. Distortion of the loaded gradient by dispersion in the gradient storage loop is studied. The effect of the most important parameters (such as flow rate, pressure and gradient steepness) is experimentally investigated. Different gradient steepnesses and volumes can be applied at different flow rates and operating pressures with a good repeatability. Due to the isobaric operation of the pumps, the gradient is monitored in real-time by a mass flow meter installed at the detector outlet. The chromatograms are then converted from time to volume-base. A separation of a 19-compound sample is performed on a 300×2.1mm column at 1000bar and on a 600×2.1mm column at 2000bar. The peak capacity was found to increase from 141 to 199 and thus scales with L as is predicted by theory. This allows to conclude that the inlet pressure for narrow-bore columns in gradient elution can be increased up to 2000bar without fundamental pressure-induced limitations. Copyright © 2016 Elsevier B.V. All rights reserved.

Non-Gradient Blue Native Polyacrylamide Gel Electrophoresis.

PubMed

Luo, Xiaoting; Wu, Jinzi; Jin, Zhen; Yan, Liang-Jun

2017-02-02

Gradient blue native polyacrylamide gel electrophoresis (BN-PAGE) is a well established and widely used technique for activity analysis of high-molecular-weight proteins, protein complexes, and protein-protein interactions. Since its inception in the early 1990s, a variety of minor modifications have been made to this gradient gel analytical method. Here we provide a major modification of the method, which we call non-gradient BN-PAGE. The procedure, similar to that of non-gradient SDS-PAGE, is simple because there is no expensive gradient maker involved. The non-gradient BN-PAGE protocols presented herein provide guidelines on the analysis of mitochondrial protein complexes, in particular, dihydrolipoamide dehydrogenase (DLDH) and those in the electron transport chain. Protocols for the analysis of blood esterases or mitochondrial esterases are also presented. The non-gradient BN-PAGE method may be tailored for analysis of specific proteins according to their molecular weight regardless of whether the target proteins are hydrophobic or hydrophilic. © 2017 by John Wiley & Sons, Inc. Copyright © 2017 John Wiley & Sons, Inc.

Generation of multiple toroidal dust vortices by a non-monotonic density gradient in a direct current glow discharge plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaur, Manjit, E-mail: manjit@ipr.res.in; Bose, Sayak; Chattopadhyay, P. K.

2015-09-15

Observation of two well-separated dust vortices in an unmagnetized parallel plate DC glow discharge plasma is reported in this paper. A non-monotonic radial density profile, achieved by an especially designed cathode structure using a concentric metallic disk and ring of different radii, is observed to produce double dust tori between cathode and anode. PIV analysis of the still images of the double tori shows oppositely rotating dust structures between the central disk and the ring. Langmuir probe measurements of background plasma shows a non-uniform plasma density profile between the disk and the ring. Location and sense of rotation of themore » dust vortices coincides with the location and direction of the radial gradient in the ion drag force caused by the radial density gradient. The experimentally observed dust vorticity matches well with the calculated one using hydrodynamic formulations with shear in ion drag dominating over the dust charge gradient. These results corroborate that a radial gradient in the ion drag force directed towards cathode is the principal cause of dust rotation.« less

Optimization and quantization in gradient symbol systems: a framework for integrating the continuous and the discrete in cognition.

PubMed

Smolensky, Paul; Goldrick, Matthew; Mathis, Donald

2014-08-01

Mental representations have continuous as well as discrete, combinatorial properties. For example, while predominantly discrete, phonological representations also vary continuously; this is reflected by gradient effects in instrumental studies of speech production. Can an integrated theoretical framework address both aspects of structure? The framework we introduce here, Gradient Symbol Processing, characterizes the emergence of grammatical macrostructure from the Parallel Distributed Processing microstructure (McClelland, Rumelhart, & The PDP Research Group, 1986) of language processing. The mental representations that emerge, Distributed Symbol Systems, have both combinatorial and gradient structure. They are processed through Subsymbolic Optimization-Quantization, in which an optimization process favoring representations that satisfy well-formedness constraints operates in parallel with a distributed quantization process favoring discrete symbolic structures. We apply a particular instantiation of this framework, λ-Diffusion Theory, to phonological production. Simulations of the resulting model suggest that Gradient Symbol Processing offers a way to unify accounts of grammatical competence with both discrete and continuous patterns in language performance. Copyright © 2013 Cognitive Science Society, Inc.

Present heat flow and paleo-geothermal regime in the Canadian Arctic margin: analysis of industrial thermal data and coalification gradients

NASA Astrophysics Data System (ADS)

Majorowicz, Jacek A.; Embry, Ashton F.

1998-06-01

Calculations of the present geothermal gradient and terrestrial heat flow were made on 156 deep wells of the Canadian Arctic Archipelago. Corrected bottom hole temperature (BHT) data and drill stem test (DST) temperatures were used to determine the thermal gradients for sites for which the quality of data was sufficient. Thermal gradients evaluated for depths below the base of permafrost for the onshore wells and below sea bottom for the offshore wells were combined with the estimates of effective thermal conductivity to approximate heat flow for these sites. The present geothermal gradient is in the 15-50 mK/m range (mean = 31 ± 7 mK/m). Present heat flow is mainly in the 35-90 mW/m 2 range (mean = 53 ± 12 mW/m 2). Maps of the present geothermal gradient and present heat flow have been constructed for the basin. The analysis of vitrinite reflectance profiles and the calculation of logarithmic coalification gradients for 101 boreholes in the Sverdrup Basin showed large variations related in many cases to regional variations of present terrestrial heat flow. Paleo-geothermal gradients estimated from these data are mostly in the range of 15-50 mK/m (mean = 28 ± 9 mK/m) and paleo-heat flow is in the 40-90 mW/m 2 range (mean = 57 ± 18 mW/m 2) related to the time of maximum burial in the Early Tertiary. Mean values of the present heat flow and paleo-heat flow for the Sverdrup Basin are almost identical considering the uncertainties of the methods used (53 ± 12 versus 57 ± 18 mW/m 2, respectively). Present geothermal gradients and paleo-geothermal gradients are also close when means are compared (31 ± 7 versus 28 ± 9 mK/m respectively). A zone of high present heat flow and a paleo-heat flow zone coincide in places with the northeastern-southwestern incipient rift landward of the Arctic margin first described by Balkwill and Fox (1982). Correlation between present heat flow and paleo-heat flow for the time of maximum burial in the earliest Tertiary suggests that the high heat flow zone has prevailed since that time.

Rapid dynamic R1 /R2 */temperature assessment: a method with potential for monitoring drug delivery.

PubMed

Lorenzato, Cyril; Oerlemans, Chris; Cernicanu, Alexandru; Ries, Mario; Denis de Senneville, Baudouin; Moonen, Chrit; Bos, Clemens

2014-11-01

Local drug delivery by hyperthermia-induced drug release from thermosensitive liposomes (TSLs) may reduce the systemic toxicity of chemotherapy, whilst maintaining or increasing its efficacy. Relaxivity contrast agents can be co-encapsulated with the drug to allow the visualization of the presence of liposomes, by means of R2 *, as well as the co-release of the contrast agent and the drug, by means of R1, on heating. Here, the mathematical method used to extract both R2 * and R1 from a fast dynamic multi-echo spoiled gradient echo (ME-SPGR) is presented and analyzed. Finally, this method is used to monitor such release events. R2 * was obtained from a fit to the ME-SPGR data. Absolute R1 was calculated from the signal magnitude changes corrected for the apparent proton density changes and a baseline Look-Locker R1 map. The method was used to monitor nearly homogeneous water bath heating and local focused ultrasound heating of muscle tissue, and to visualize the release of a gadolinium chelate from TSLs in vitro. R2 *, R1 and temperature maps were measured with a 5-s temporal resolution. Both R2 *and R1 measured were found to change with temperature. The dynamic R1 measurements after heating agreed with the Look-Locker R1 values if changes in equilibrium magnetization with temperature were considered. Release of gadolinium from TSLs was detected by an R1 increase near the phase transition temperature, as well as a shallow R2 * increase. Simultaneous temperature, R2 * and R1 mapping is feasible in real time and has the potential for use in image-guided drug delivery studies. Copyright © 2014 John Wiley & Sons, Ltd.

Probabilistic inversion of electrical resistivity data from bench-scale experiments: On model parameterization for CO2 sequestration monitoring

NASA Astrophysics Data System (ADS)

Breen, S. J.; Lochbuehler, T.; Detwiler, R. L.; Linde, N.

2013-12-01

Electrical resistivity tomography (ERT) is a well-established method for geophysical characterization and has shown potential for monitoring geologic CO2 sequestration, due to its sensitivity to electrical resistivity contrasts generated by liquid/gas saturation variability. In contrast to deterministic ERT inversion approaches, probabilistic inversion provides not only a single saturation model but a full posterior probability density function for each model parameter. Furthermore, the uncertainty inherent in the underlying petrophysics (e.g., Archie's Law) can be incorporated in a straightforward manner. In this study, the data are from bench-scale ERT experiments conducted during gas injection into a quasi-2D (1 cm thick), translucent, brine-saturated sand chamber with a packing that mimics a simple anticlinal geological reservoir. We estimate saturation fields by Markov chain Monte Carlo sampling with the MT-DREAM(ZS) algorithm and compare them quantitatively to independent saturation measurements from a light transmission technique, as well as results from deterministic inversions. Different model parameterizations are evaluated in terms of the recovered saturation fields and petrophysical parameters. The saturation field is parameterized (1) in cartesian coordinates, (2) by means of its discrete cosine transform coefficients, and (3) by fixed saturation values and gradients in structural elements defined by a gaussian bell of arbitrary shape and location. Synthetic tests reveal that a priori knowledge about the expected geologic structures (as in parameterization (3)) markedly improves the parameter estimates. The number of degrees of freedom thus strongly affects the inversion results. In an additional step, we explore the effects of assuming that the total volume of injected gas is known a priori and that no gas has migrated away from the monitored region.

Special Test Methods for Batteries

NASA Technical Reports Server (NTRS)

Gross, S.

1984-01-01

Various methods are described for measuring heat generation in primary and secondary batteries as well as the specific heat of batteries and cell thermal conductance. Problems associated with determining heat generation in large batteries are examined. Special attention is given to monitoring temperature gradients in nickel cadmium cells, the use of auxiliary electrodes for conducting tests on battery charge control, evaluating the linear sweep of current from charge to discharge, and determining zero current voltage. The fast transient behavior of batteries in the microsecond range, and the electrical conductance of nickel sinters in the thickness direction are also considered. Mechanical problems experienced in the vibration of Ni-Cd batteries and tests to simulate cyclic fatigue of the steel table connecting the plates to the comb are considered. Methods of defining the distribution of forces when cells are compressed during battery packaging are also explored.

Special test methods for batteries

NASA Astrophysics Data System (ADS)

Gross, S.

1984-09-01

Various methods are described for measuring heat generation in primary and secondary batteries as well as the specific heat of batteries and cell thermal conductance. Problems associated with determining heat generation in large batteries are examined. Special attention is given to monitoring temperature gradients in nickel cadmium cells, the use of auxiliary electrodes for conducting tests on battery charge control, evaluating the linear sweep of current from charge to discharge, and determining zero current voltage. The fast transient behavior of batteries in the microsecond range, and the electrical conductance of nickel sinters in the thickness direction are also considered. Mechanical problems experienced in the vibration of Ni-Cd batteries and tests to simulate cyclic fatigue of the steel table connecting the plates to the comb are considered. Methods of defining the distribution of forces when cells are compressed during battery packaging are also explored.

Focused US system for MR imaging-guided tumor ablation.

PubMed

Cline, H E; Hynynen, K; Watkins, R D; Adams, W J; Schenck, J F; Ettinger, R H; Freund, W R; Vetro, J P; Jolesz, F A

1995-03-01

To measure the performance characteristics of a focused ultrasound (US) system for magnetic resonance (MR) imaging-guided tumor ablation. The authors constructed a focused US system for MR imaging-guided tumor ablation. The location of the heated region and thermal dose were monitored with temperature-sensitive MR images obtained in phantoms and rabbit skeletal muscle after application of each sonic pulse. The region heated by the focused ultrasound beam was within 1 mm of that observed on temperature-sensitive fast gradient-echo MR images of in vivo rabbit skeletal muscle. Analysis of heat flow and the rate of coagulation necrosis provided an estimate of the size of the ablated region that was in agreement with experimental findings. MR imaging provides target definition and control for thermal therapy in regions of variable perfusion or in tissues that are not well characterized.

Differential responses of soil microbial biomass, diversity, and compositions to altitudinal gradients depend on plant and soil characteristics.

PubMed

Ren, Chengjie; Zhang, Wei; Zhong, ZeKun; Han, Xinhui; Yang, Gaihe; Feng, Yongzhong; Ren, Guangxin

2018-01-01

Alt'itudinal gradients strongly affect plant biodiversity, but the effects on microbial patterns remain unclear, especially in the large scale. We therefore designed an altitudinal gradient experiment that covered three climate zones to monitor soil microbial community dynamics and to compare those with plant and soil characteristics. Illumina sequencing of the 16S rRNA gene and ITS gene was used to analyze soil microbial (bacterial and fungal) diversity and composition, and fumigation-extraction was used to determine microbial biomass; the plant community metrics (i.e., percent cover, Shannon-Wiener, grass biomass, and carbon/nitrogen in leaf and biomass) and soil properties (i.e., soil moisture, soil temperature, bulk density, organic carbon, total nitrogen, and available nitrogen) were determined. The results showed that carbon/nitrogen in microbial biomass was higher at medium altitude and was positively related to carbon and nitrogen in both soil and grass biomass along the altitudinal gradients. Soil bacterial alpha diversity was significantly higher at medium altitude but fungal alpha diversity did not affected by altitudinal gradients; the effect of altitudinal gradients on bacterial beta diversity was larger than that on fungal beta diversity, although both groups were significantly affected by altitudinal gradients. Moreover, Alpha-proteobacteria, Beta-proteobacteria, and Gemmatimonadetes were significantly more abundant in higher altitude than in lower altitude, both Acidobacteria and Actinobacteria significantly declined with increasing altitude; other bacterial taxa such as Chloroflexi, Nitrospirae, Gamma-proteobacteria, and Delta-proteobacteria were significantly higher at medium altitudes. For fungal taxa, Basidiomycota and Ascomycota were the dominant phyla and responded insignificantly to the altitudinal gradients. The responses of microbial alpha diversity were mostly associated with plant Shannon index, organic carbon, and total nitrogen, whereas microbial beta diversity and composition mainly depended on soil moisture and temperature. Overall, these results suggest that soil bacteria rather than fungi can reflect changes in plant and soil characteristics along altitudinal gradients. Copyright © 2017 Elsevier B.V. All rights reserved.

Testing thermal gradient driving force for grain boundary migration using molecular dynamics simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bai, Xian-Ming; Zhang, Yongfeng; Tonks, Michael R.

2015-02-01

Strong thermal gradients in low-thermal-conductivity ceramics may drive extended defects, such as grain boundaries and voids, to migrate in preferential directions. In this work, molecular dynamics simulations are conducted to study thermal gradient driven grain boundary migration and to verify a previously proposed thermal gradient driving force equation, using uranium dioxide as a model system. It is found that a thermal gradient drives grain boundaries to migrate up the gradient and the migration velocity increases under a constant gradient owing to the increase in mobility with temperature. Different grain boundaries migrate at very different rates due to their different intrinsicmore » mobilities. The extracted mobilities from the thermal gradient driven simulations are compared with those calculated from two other well-established methods and good agreement between the three different methods is found, demonstrating that the theoretical equation of the thermal gradient driving force is valid, although a correction of one input parameter should be made. The discrepancy in the grain boundary mobilities between modeling and experiments is also discussed.« less

Simultaneous determination of antidementia drugs in human plasma for therapeutic drug monitoring.

PubMed

Noetzli, Muriel; Choong, Eva; Ansermot, Nicolas; Eap, Chin B

2011-04-01

A simple liquid chromatography mass spectrometry method was developed and validated for the simultaneous determination of antidementia drugs, including donepezil, galantamine, rivastigmine and its major metabolite, NAP 226-90, and memantine. A solid phase extraction procedure with a mixed-mode sorbent was used to isolate the drugs from 0.5 mL human plasma. Reverse phase chromatographic separation of the compounds was obtained with a gradient elution of an ammonium acetate buffer at pH 9.3 and acetonitrile and the analytes were detected by mass spectrometry in the single ion monitoring mode. The method was validated according to the recommendations of the Food and Drug Administration, including assessment of trueness (-8.0% to +10.7%), imprecision (repeatability: 1.1-4.9%, intermediate imprecision: 2.1-8.5%), selectivity and matrix effects variability (less than 6%) as well as short- and long-term stability in plasma. The calibration ranges were from 1 ng/mL to 300 ng/mL (rivastigmine and memantine) and 2 ng/mL to 300 ng/mL (donepezil, galantamine, and NAP 226-90). The method was successfully applied to patients' samples and might contribute to evaluate whether a therapeutic drug monitoring-guided dose adjustment of antidementia drugs could contribute to minimize the risk of adverse reactions and to increase the probability of efficient therapeutic response.

Thermally tailored gradient topography surface on elastomeric thin films.

PubMed

Roy, Sudeshna; Bhandaru, Nandini; Das, Ritopa; Harikrishnan, G; Mukherjee, Rabibrata

2014-05-14

We report a simple method for creating a nanopatterned surface with continuous variation in feature height on an elastomeric thin film. The technique is based on imprinting the surface of a film of thermo-curable elastomer (Sylgard 184), which has continuous variation in cross-linking density introduced by means of differential heating. This results in variation of viscoelasticity across the length of the surface and the film exhibits differential partial relaxation after imprinting with a flexible stamp and subjecting it to an externally applied stress for a transient duration. An intrinsic perfect negative replica of the stamp pattern is initially created over the entire film surface as long as the external force remains active. After the external force is withdrawn, there is partial relaxation of the applied stresses, which is manifested as reduction in amplitude of the imprinted features. Due to the spatial viscoelasticity gradient, the extent of stress relaxation induced feature height reduction varies across the length of the film (L), resulting in a surface with a gradient topography with progressively varying feature heights (hF). The steepness of the gradient can be controlled by varying the temperature gradient as well as the duration of precuring of the film prior to imprinting. The method has also been utilized for fabricating wettability gradient surfaces using a high aspect ratio biomimetic stamp. The use of a flexible stamp allows the technique to be extended for creating a gradient topography on nonplanar surfaces as well. We also show that the gradient surfaces with regular structures can be used in combinatorial studies related to pattern directed dewetting.

Inhaled Beta Agonist Bronchodilator Does Not Affect Trans-diaphragmatic Pressure Gradient but Decreases Lower Esophageal Sphincter Retention Pressure in Patients with Chronic Obstructive Pulmonary Disease (COPD) and Gastroesophageal Reflux Disease (GERD).

PubMed

Del Grande, Leonardo M; Herbella, Fernando A M; Bigatao, Amilcar M; Jardim, Jose R; Patti, Marco G

2016-10-01

Chronic obstructive pulmonary disease (COPD) patients have a high incidence of gastroesophageal reflux disease (GERD) whose pathophysiology seems to be linked to an increased trans-diaphragmatic pressure gradient and not to a defective esophagogastric barrier. Inhaled beta agonist bronchodilators are a common therapy used by patients with COPD. This drug knowingly not only leads to a decrease in the lower esophageal sphincter (LES) resting pressure, favoring GERD, but also may improve ventilatory parameters, therefore preventing GERD. This study aims to evaluate the effect of inhaled beta agonist bronchodilators on the trans-diaphragmatic pressure gradient and the esophagogastric barrier. We studied 21 patients (mean age 67 years, 57 % males) with COPD and GERD. All patients underwent high-resolution manometry and esophageal pH monitoring. Abdominal and thoracic pressure, trans-diaphragmatic pressure gradient (abdominal-thoracic pressure), and the LES retention pressure (LES basal pressure-transdiaphragmatic gradient) were measured before and 5 min after inhaling beta agonist bronchodilators. The administration of inhaled beta agonist bronchodilators leads to the following: (a) a simultaneous increase in abdominal and thoracic pressure not affecting the trans-diaphragmatic pressure gradient and (b) a decrease in the LES resting pressure with a reduction of the LES retention pressure. In conclusion, inhaled beta agonist bronchodilators not only increase the thoracic pressure but also lead to an increased abdominal pressure favoring GERD by affecting the esophagogastric barrier.

Community-based Monitoring of Water Resources in Remote Mountain Regions

NASA Astrophysics Data System (ADS)

Buytaert, W.; Hannah, D. M.; Dewulf, A.; Clark, J.; Zulkafli, Z. D.; Karpouzoglou, T.; Mao, F.; Ochoa-Tocachi, B. F.

2016-12-01

Remote mountain regions are often represented by pockets of poverty combined with accelerated environmental change. The combination of harsh climatic and topographical conditions with limited infrastructure puts severe pressures on local livelihoods, many of which rely strongly on local ecosystem services (ESS) such as agricultural production and water supply. It is therefore paramount to optimise the management of ESS for the benefit of local people. This is hindered by a scarcity of quantitative data about physical processes such as precipitation and river flow as well as qualitative data concerning the management of water and land. National and conventional scientific monitoring networks tend to be insufficient to cover adequately the spatial and temporal gradients. Additionally, the data that are being collected often fail to be converted into locally relevant and actionable knowledge for ESS management. In such conditions, community-based monitoring of natural resources may be an effective way to reduce this knowledge gap. The participatory nature of such monitoring also enhances knowledge co-production and integration in locally-based decision-making processes. Here, we present the results of a 4-year consortium project on the use of citizen science technologies for ecosystem services management (Mountain-EVO). The project analyzed ecosystem service dynamics and decision-making processes and implemented a comparative analysis of experiments with community-based monitoring of water resources in 4 remote mountain regions, i.e. Peru, Nepal, Kyrgyzstan, and Ethiopia. We find that community-based monitoring can have a transformative impact on local ESS management, because of its potential to be more inclusive, polycentric, and context-driven as compared to conventional monitoring. However, the results and effectiveness of community-based approaches depend strongly on the natural and socio-economic boundary conditions. As such, this requires a tailored and bottom-up approach to implementation, which ideally isrooted in locally-based set of actors that can act as catalysts for knowledge co-production between the scientific community and local ESS users.

Selected natural attenuation monitoring data, Operable Unit 1, Naval Undersea Warfare Center, Division Keyport, Washington, June 2001

USGS Publications Warehouse

Dinico, Richard Steven

2003-01-01

Previous investigations have shown that natural attenuation and biodegradation of chlorinated volatile organic compounds (CVOCs) are substantial in shallow ground water beneath the 9-acre former landfill at Operable Unit 1 (OU 1), Naval Undersea Warfare Center (NUWC), Division Keyport, Washington. The U.S. Geological Survey (USGS) has continued to monitor ground-water geochemistry to assure that conditions remain favorable for contaminant biodegradation. This report presents the ground-water geochemical and selected CVOC data collected at OU 1 by the USGS during June 11-14, 2001 in support of the long-term monitoring for natural attenuation. Overall, the June 2001 data indicate that redox conditions in the upper aquifer remain favorable for reductive dechlorination of CVOCs because strongly reducing conditions persisted beneath much of the former landfill. Redox conditions in the intermediate aquifer down gradient of the landfill appear to have become more favorable for reductive dechlorination because June 2001 dissolved hydrogen concentrations indicated strongly reducing conditions there for the first time. Although changes in redox conditions were observed at certain wells during 2001, a longer monitoring period is needed to ascertain if phytoremediation activities are affecting the ground-water chemistry. A minor change to future monitoring is proposed. Filtered organic carbon (previously referred to as dissolved, and defined as that which passes through a 0.45-micrometer membrane filter) should be analyzed in the future rather than unfiltered (previously referred to as total) organic carbon because the filtered analysis may be a better measure of bioavailable organic carbon. Unfiltered and filtered organic carbon data were collected during June 2001 for comparison. Filtered organic carbon data collected in the future could be reasonably compared with historical unfiltered organic carbon data by multiplying the historical data by a factor of about 0.9.

Remote sensing of exposure to NO2: Satellite versus ground-based measurement in a large urban area

NASA Astrophysics Data System (ADS)

Bechle, Matthew J.; Millet, Dylan B.; Marshall, Julian D.

2013-04-01

Remote sensing may be a useful tool for exploring spatial variability of air pollution exposure within an urban area. To evaluate the extent to which satellite data from the Ozone Monitoring Instrument (OMI) can resolve urban-scale gradients in ground-level nitrogen dioxide (NO2) within a large urban area, we compared estimates of surface NO2 concentrations derived from OMI measurements and US EPA ambient monitoring stations. OMI, aboard NASA's Aura satellite, provides daily afternoon (˜13:30 local time) measurements of NO2 tropospheric column abundance. We used scaling factors (surface-to-column ratios) to relate satellite column measurements to ground-level concentrations. We compared 4138 sets of paired data for 25 monitoring stations in the South Coast Air Basin of California for all of 2005. OMI measurements include more data gaps than the ground monitors (60% versus 5% of available data, respectively), owing to cloud contamination and imposed limits on pixel size. The spatial correlation between OMI columns and corrected in situ measurements is strong (r = 0.93 for annual average data), indicating that the within-urban spatial signature of surface NO2 is well resolved by the satellite sensor. Satellite-based surface estimates employing scaling factors from an urban model provide a reliable measure (annual mean bias: -13%; seasonal mean bias: <1% [spring] to -22% [fall]) of fine-scale surface NO2. We also find that OMI provides good spatial density in the study region (average area [km2] per measurement: 730 for the satellite sensor vs. 1100 for the monitors). Our findings indicate that satellite observations of NO2 from the OMI sensor provide a reliable measure of spatial variability in ground-level NO2 exposure for a large urban area.

Caudal Regulates the Spatiotemporal Dynamics of Pair-Rule Waves in Tribolium

PubMed Central

El-Sherif, Ezzat; Zhu, Xin; Fu, Jinping; Brown, Susan J.

2014-01-01

In the short-germ beetle Tribolium castaneum, waves of pair-rule gene expression propagate from the posterior end of the embryo towards the anterior and eventually freeze into stable stripes, partitioning the anterior-posterior axis into segments. Similar waves in vertebrates are assumed to arise due to the modulation of a molecular clock by a posterior-to-anterior frequency gradient. However, neither a molecular candidate nor a functional role has been identified to date for such a frequency gradient, either in vertebrates or elsewhere. Here we provide evidence that the posterior gradient of Tc-caudal expression regulates the oscillation frequency of pair-rule gene expression in Tribolium. We show this by analyzing the spatiotemporal dynamics of Tc-even-skipped expression in strong and mild knockdown of Tc-caudal, and by correlating the extension, level and slope of the Tc-caudal expression gradient to the spatiotemporal dynamics of Tc-even-skipped expression in wild type as well as in different RNAi knockdowns of Tc-caudal regulators. Further, we show that besides its absolute importance for stripe generation in the static phase of the Tribolium blastoderm, a frequency gradient might serve as a buffer against noise during axis elongation phase in Tribolium as well as vertebrates. Our results highlight the role of frequency gradients in pattern formation. PMID:25329152

SW New Mexico BHT geothermal gradient calculations

DOE Data Explorer

Shari Kelley

2015-07-24

This file contains a compilation of BHT data from oil wells in southwestern New Mexico. Surface temperature is calculated using the collar elevation. An estimate of geothermal gradient is calculated using the estimated surface temperature and the uncorrected BHT data.

Are two systemic fish assemblage sampling programmes on the upper Mississippi River telling us the same thing?

USGS Publications Warehouse

Dukerschein, J.T.; Bartels, A.D.; Ickes, B.S.; Pearson, M.S.

2013-01-01

We applied an Index of Biotic Integrity (IBI) used on Wisconsin/Minnesota waters of the upper Mississippi River (UMR) to compare data from two systemic sampling programmes. Ability to use data from multiple sampling programmes could extend spatial and temporal coverage of river assessment and monitoring efforts. We normalized for effort and tested fish community data collected by the Environmental Monitoring and Assessment Program-Great Rivers Ecosystems (EMAP-GRE) 2004–2006 and the Long Term Resource Monitoring Program (LTRMP) 1993–2006. Each programme used daytime electrofishing along main channel borders but with some methodological and design differences. EMAP-GRE, designed for baseline and, eventually, compliance monitoring, used a probabilistic, continuous design. LTRMP, designed primarily for baseline and trend monitoring, used a stratified random design in five discrete study reaches. Analysis of similarity indicated no significant difference between EMAP-GRE and LTRMP IBI scores (n=238; Global R= 0.052; significance level=0.972). Both datasets distinguished clear differences only between 'Fair' and 'Poor' condition categories, potentially supporting a 'pass–fail' assessment strategy. Thirteen years of LTRMP data demonstrated stable IBI scores through time in four of five reaches sampled. LTRMP and EMAPGRE IBI scores correlated along the UMR's upstream to downstream gradient (df [3, 25]; F=1.61; p=0.22). A decline in IBI scores from upstream to downstream was consistent with UMR fish community studies and a previous, empirically modelled human disturbance gradient. Comparability between EMAP-GRE (best upstream to downstream coverage) and LTRMP data (best coverage over time and across the floodplain) supports a next step of developing and testing a systemic, multi-metric fish index on the UMR that both approaches could inform.

Socioeconomic inequalities in parent-reported and teacher-reported psychological well-being.

PubMed

Lewis, Hannah; Hope, Steven; Pearce, Anna

2015-01-01

To determine whether there are differences in the social gradient of parent-reported and teacher-reported child psychological well-being. Secondary data analysis comparing ratings of child psychological well-being (Strengths and Difficulties Questionnaire, SDQ) in the UK Millennium Cohort Study at 7 years by socioeconomic circumstances (SEC). A number of measures of SEC were tested; results are reported for maternal education. From a sample of 13,168 singletons who participated at the age of 7 years, complete data were available for 8207 children. There was a social gradient in SDQ scores reported by parents and teachers, with 'borderline/abnormal' scores more prevalent in children with lower-educated mothers. However, the gradient was more marked in parent report compared with teacher report, and discrepancies between parent and teacher reports were greatest for children from higher SECs. The social gradient in child psychological well-being, although present, was weaker in teacher report compared with parent report. This may be because children behave differently in school and home settings, or parents and teachers demonstrate reporting bias. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

First gas-phase metallicity gradients of 0.1 ≲ z ≲ 0.8 galaxies with MUSE

NASA Astrophysics Data System (ADS)

Carton, David; Brinchmann, Jarle; Contini, Thierry; Epinat, Benoît; Finley, Hayley; Richard, Johan; Patrício, Vera; Schaye, Joop; Nanayakkara, Themiya; Weilbacher, Peter M.; Wisotzki, Lutz

2018-05-01

Galaxies at low-redshift typically possess negative gas-phase metallicity gradients (centres more metal-rich than their outskirts). Whereas, it is not uncommon to observe positive metallicity gradients in higher-redshift galaxies (z ≳ 0.6). Bridging these epochs, we present gas-phase metallicity gradients of 84 star-forming galaxies between 0.08 < z < 0.84. Using the galaxies with reliably determined metallicity gradients, we measure the median metallicity gradient to be negative (-0.039^{+0.007}_{-0.009} dex/kpc). Underlying this, however, is significant scatter: (8 ± 3)% [7] of galaxies have significantly positive metallicity gradients, (38 ± 5)% [32] have significantly negative gradients, (31 ± 5)% [26] have gradients consistent with being flat. (The remaining (23 ± 5)% [19] have unreliable gradient estimates.) We notice a slight trend for a more negative metallicity gradient with both increasing stellar mass and increasing star formation rate (SFR). However, given the potential redshift and size selection effects, we do not consider these trends to be significant. Indeed, once we normalize the SFR relative to that of the main sequence, we do not observe any trend between the metallicity gradient and the normalized SFR. This is contrary to recent studies of galaxies at similar and higher redshifts. We do, however, identify a novel trend between the metallicity gradient of a galaxy and its size. Small galaxies (rd < 3 kpc) present a large spread in observed metallicity gradients (both negative and positive gradients). In contrast, we find no large galaxies (rd > 3 kpc) with positive metallicity gradients, and overall there is less scatter in the metallicity gradient amongst the large galaxies. These large (well-evolved) galaxies may be analogues of present-day galaxies, which also show a common negative metallicity gradient.

Monitoring Biological Activity at Geothermal Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peter Pryfogle

2005-09-01

The economic impact of microbial growth in geothermal power plants has been estimated to be as high as $500,000 annually for a 100 MWe plant. Many methods are available to monitor biological activity at these facilities; however, very few plants have any on-line monitoring program in place. Metal coupon, selective culturing (MPN), total organic carbon (TOC), adenosine triphosphate (ATP), respirometry, phospholipid fatty acid (PLFA), and denaturing gradient gel electrophoresis (DGGE) characterizations have been conducted using water samples collected from geothermal plants located in California and Utah. In addition, the on-line performance of a commercial electrochemical monitor, the BIoGEORGE?, has beenmore » evaluated during extended deployments at geothermal facilities. This report provides a review of these techniques, presents data on their application from laboratory and field studies, and discusses their value in characterizing and monitoring biological activities at geothermal power plants.« less

Spiral passive electromagnetic sensor (SPES) for smart sensing and de-icing

NASA Astrophysics Data System (ADS)

Iervolino, Onorio; Meo, Michele

2015-04-01

The objective of this work was to develop a wireless Spiral Passive Electromagnetic Sensor (SPES) to monitor the complex permittivity of a surrounding medium. The sensor is a self-resonating planar pattern of electrically conductive material. Investigation were conducted to demonstrate the capability of the SPES to monitor humidity and temperature gradients, and acting as an ice protection tool. An oscillating signal is used to interrogate remotely the sensor with a single loop antenna or wiring it directly to a spectrum analyser and monitoring the backscattering signal. The excited sensor responds with its own resonant frequency, amplitude and bandwidth that can be correlated to physical quantities to be monitored. Our studies showed the capability of the sensor to monitor temperature and humidity changes in composite materials and uniformly produce induction heating when the conductive path is activated by an external electric power supply that can be used for deicing of aircraft structures.

Combinational concentration gradient confinement through stagnation flow.

PubMed

Alicia, Toh G G; Yang, Chun; Wang, Zhiping; Nguyen, Nam-Trung

2016-01-21

Concentration gradient generation in microfluidics is typically constrained by two conflicting mass transport requirements: short characteristic times (τ) for precise temporal control of concentration gradients but at the expense of high flow rates and hence, high flow shear stresses (σ). To decouple the limitations from these parameters, here we propose the use of stagnation flows to confine concentration gradients within large velocity gradients that surround the stagnation point. We developed a modified cross-slot (MCS) device capable of feeding binary and combinational concentration sources in stagnation flows. We show that across the velocity well, source-sink pairs can form permanent concentration gradients. As source-sink concentration pairs are continuously supplied to the MCS, a permanently stable concentration gradient can be generated. Tuning the flow rates directly controls the velocity gradients, and hence the stagnation point location, allowing the confined concentration gradient to be focused. In addition, the flow rate ratio within the MCS rapidly controls (τ ∼ 50 ms) the location of the stagnation point and the confined combinational concentration gradients at low flow shear (0.2 Pa < σ < 2.9 Pa). The MCS device described in this study establishes the method for using stagnation flows to rapidly generate and position low shear combinational concentration gradients for shear sensitive biological assays.

Internal and External Temperature Monitoring of a Li-Ion Battery with Fiber Bragg Grating Sensors

PubMed Central

Novais, Susana; Nascimento, Micael; Grande, Lorenzo; Domingues, Maria Fátima; Antunes, Paulo; Alberto, Nélia; Leitão, Cátia; Oliveira, Ricardo; Koch, Stephan; Kim, Guk Tae; Passerini, Stefano; Pinto, João

2016-01-01

The integration of fiber Bragg grating (FBG) sensors in lithium-ion cells for in-situ and in-operando temperature monitoring is presented herein. The measuring of internal and external temperature variations was performed through four FBG sensors during galvanostatic cycling at C-rates ranging from 1C to 8C. The FBG sensors were placed both outside and inside the cell, located in the center of the electrochemically active area and at the tab-electrode connection. The internal sensors recorded temperature variations of 4.0 ± 0.1 °C at 5C and 4.7 ± 0.1 °C at 8C at the center of the active area, and 3.9 ± 0.1 °C at 5C and 4.0 ± 0.1 °C at 8C at the tab-electrode connection, respectively. This study is intended to contribute to detection of a temperature gradient in real time inside a cell, which can determine possible damage in the battery performance when it operates under normal and abnormal operating conditions, as well as to demonstrate the technical feasibility of the integration of in-operando microsensors inside Li-ion cells. PMID:27589749

Integrated environmental monitoring and simulation system for use as a management decision support tool in urban areas.

PubMed

Fatta, D; Naoum, D; Loizidou, M

2002-04-01

Leachates are generated as a result of water or other liquid passing through waste at a landfill site. These contaminated liquids originate from a number of sources, including the water produced during the decomposition of the waste as well as rain-fall which penetrates the waste and dissolves the material with which it comes into contact. The penetration of the rain-water depends on the nature of the landfill (e.g. surface characteristics, type and quantity of vegetation, gradient of layers, etc). The uncontrolled infiltration of leachate into the vadose (unsaturated) zone and finally into the saturated zone (groundwater) is considered to be the most serious environmental impact of a landfill. In the present paper the water flow and the pollutant transport characteristics of the Ano Liosia Landfill site in Athens (Greece) were simulated by creating a model of groundwater flows and contaminant transport. A methodology for the model is presented. The model was then integrated into the Ecosim system which is a prototype funded by the EU, (Directorate General XIII: Telematics and Environment). This is an integrated environmental monitoring and modeling system, which supports the management of environmental planning in urban areas.

The Goldilocks Principle and Rapid Evolution of Antibiotic Resistance in Bacteria

NASA Astrophysics Data System (ADS)

Zhang, Qiucen; Austin, Robert

2011-03-01

Goldilocks sampled the three bear's wares for the ``just right'' combination of taste, fit and comfort. Like Goldilocks's need for the just right parameters, evolution proceeds most rapidly when there is the just right combination of a large number of mutants and rapid fixation of the mutants. We show here using a two-dimensional micro-ecology that it is possible to fix resistance to the powerful antibiotic ciprofloxacin (Cipro) in wild-type E. coli in 10 hours through a combination of extremely high population gradients, which generate rapid fixation, convolved with the just right level of antibiotic which generates a large number of mutants and the motility of the organism. Although evolution occurs in well-stirred chemostats without such Goldilocks conditions, natural environments are rarely well stirred in nature.For complex environments such as the Galapagos Islands, spatial population gradients and movement of mutants along these population gradients can be as important as genomic heterogeneity in setting the speed of evolution. The design of our micro-ecology is unique in that it provides two overlapping gradients, one an emergent and self generated bacterial population gradient due to food restriction and the other a mutagenic antibiotic gradient. Further, it exploits the motility of the bacteria moving across these gradients to drive the rate of resistance to Cipro to extraordinarily high rates. The research described was supported by Award Number U54CA143803 from the National Cancer Institute.

Introduction to: Atlantic Meridional Overturning Circulation(AMOC)

NASA Technical Reports Server (NTRS)

Hakkinen, Sirpa; Carton, James A.

2011-01-01

A striking conclusion of the Intergovernmental Panel on Climate Change 2007 report is the crucial role that the Atlantic Meridional Overturning Circulation (AMOC) may play in anthropogenic climate change. However, these IPCC coupled climate simulations show a broad range of uncertainty in the magnitude and timing of AMOC transport change ranging from none to nearly complete collapse within the 21st century. The potential consequences of large changes in the characteristics of AMOC have motivated the creation in the United States of an interagency program and implementation plan to develop monitoring and prediction capabilities for the AMOC This program parallels the development of substantial monitoring efforts by European, South American and African countries -- notably the UK Rapid and Rapid-Watch programs. The papers contained in this volume are derived from presentations at the First U.S. Atlantic Meridional Overturning Circulation (AMOC) Meeting held 4 - 6 May, 2009 to review the US implementation plan and its coordination with other monitoring activities. The Atlantic Meridional Overturning Circulation consists of multiple components illustrated in an attached figure. Water enters the South Atlantic at upper and intermediate depths through both western and eastern routes (where eddy transport is especially important) and is transported northward across the equator, where it recirculates within the northern subtropical and subpolar gyres. The northern end is defined by the sinking regions of the Nordic Seas and the Labrador Sea where the waters that eventually form the upper and lower branches of North Atlantic Deep Water are conditioned. High surface salinities, the result of high net evaporation in the tropics and subtropics (including the Mediterranean Sea), and presence of regions of the Arctic Ocean that remain ice-free even in winter allow for the rapid cooling and thus densification of surface water. This dense surface water becomes the source of deep water formation in the sinking regions. In addition to transporting mass, the AMOC transports roughly half of the total amount of heat carried northward through the northern subtropics (down the temperature-gradient) by the ocean. In contrast in the Southern Hemisphere AMOC transports heat up-gradient from the cool Circumpolar Current to the warm tropics. Paleoevidence suggests that AMOC heat transport in the two hemispheres has varied over time in ways intimately tied to millennial changes in the Earth's climate. In one example, the abrupt Younger Dryas spell of cold weather over the North Atlantic, which began 13,000 years ago, has generally been linked to a millennial shutdown of the AMOC as a result of massive freshwater discharge from the North American continent. The current AMOC monitoring array consists of a series of instrumented transects located across key passages (see Cunningham et al., 2010 for a recent review). In the Arctic and sub-Arctic, transects cross Fram Strait, Denmark Strait and the Faroe Channel (connecting Greenland, Iceland, and the United Kingdom), as well as the entrance to the Labrador Sea. Further south and extending outwards from the east coast of North America there are a series of monitoring arrays including arrays of the Canadian Atlantic Zone Monitoring Program, deployments of the Rapid Western Atlantic Variability Experiment (WAVE), Line W at 39 N, as well as the Rapid-MOC moored array. The latter spans the entire Atlantic basin along 26.5 N. At tropical latitudes we have the Meridional Overturning Variability Experiment (MOVE) array at 16 N, while in the Southern Hemisphere a corresponding basin-spanning transect is being established at the latitude of Cape of Good Hope, complemented by arrays at Drake Passage.

Comparison of Fracture Gradient Methods for the FutureGen 2.0 Carbon Storage Site, Ill., USA.

NASA Astrophysics Data System (ADS)

Appriou, D.; Spane, F.; Wurstner White, S.; Kelley, M. E.; Sullivan, E. C.; Bonneville, A.; Gilmore, T. J.

2014-12-01

As part of a first-of-its-kind carbon dioxide storage project, FutureGen Industrial Alliance is planning to inject 1.1 MMt/yr of supercritical CO2 over a 20-year period within a 1240 m deep saline aquifer (Mount Simon Sandstone) located in Morgan County, Illinois, USA. Numerous aspects of the design and operational activities of the CO2 storage site are dependent on the geomechanical properties of the targeted reservoir zone, as well as of the overlying confining zone and the underlying crystalline Precambrian basement. Detailed determination of the state-of-stress within the subsurface is of paramount importance in successfully designing well drilling/completion aspects, as well as assessing the risk of induced seismicity and the potential for creating and/or reopening pre-existing fractures; all of which help ensure the safe long-term storage of injected CO2. The quantitative determination of the subsurface fracture gradient is one of the key geomechanical parameters for the site injection design and operational limits (e.g., maximum safe injection pressure). A characterization well drilled in 2011 provides subsurface geomechanical characterization information for the FutureGen 2.0 site, and includes: 1) continuous elastic properties inferred from sonic/acoustic wireline logs 2) discrete depth geomechanical laboratory core measurements and 3) results obtained from hydraulic fracturing tests of selected borehole/depth-intervals. In this paper, the precise fracture gradients derived from borehole geomechanical test results are compared with semi-empirical, fracture gradient calculation/relationships based on elastic property wireline surveys and laboratory geomechanical core test results. Implications for using various fracture-gradients obtained from the different methods are assessed using PNNL's subsurface multiphase flow and transport simulator STOMP-CO2. The implications for operational activities at the site (based on using different fracture gradients) are also discussed.

Hydrologic assessment of three drainage basins in the Pinelands of southern New Jersey, 2004-06

USGS Publications Warehouse

Walker, Richard L.; Nicholson, Robert S.; Storck, Donald A.

2011-01-01

The New Jersey Pinelands is an ecologically diverse area in the southern New Jersey Coastal Plain, most of which overlies the Kirkwood-Cohansey aquifer system. The demand for groundwater from this aquifer system is increasing as local development increases. Because any increase in groundwater withdrawals has the potential to affect streamflows and wetland water levels, and ultimately threaten the ecological health and diversity of the Pinelands ecosystem, the U.S. Geological Survey, in cooperation with the New Jersey Pinelands Commission, began a multi-phase hydrologic investigation in 2004 to characterize the hydrologic system supporting the aquatic and wetland communities of the New Jersey Pinelands area (Pinelands). The current investigation of the hydrology of three representative drainage basins in the Pinelands (Albertson Brook, McDonalds Branch, and Morses Mill Stream basins) included a compilation of existing data; collection of water-level and streamflow data; mapping of the water-table altitude and depth to the water table; and analyses of water-level and streamflow variability, subsurface gradients and flow patterns, and water budgets. During 2004-06, a hydrologic database of existing and new data from wells and stream sites was compiled. Methods of data collection and analysis were defined, and data networks consisting of 471 wells and 106 surface-water sites were established. Hydrographs from 26 water-level-monitoring wells and four streamflow-gaging stations were analyzed to show the response of water levels and streamflow to precipitation and recharge with respect to the locations of these wells and streams within each basin. Water-level hydrographs show varying hydraulic gradients and flow potentials, and indicate that responses to recharge events vary with well depth and proximity to recharge and discharge areas. Results of the investigation provide a detailed characterization of hydrologic conditions, processes, and relations among the components of the hydrologic cycle in the Pinelands. In the Pinelands, recharge replenishes the aquifer system and contributes to groundwater flow, most of which moves to wetlands and surface water where natural discharge occurs. Some groundwater flow is intercepted by supply wells. Recharge rates generally are highest during the non-growing season and are inversely related to evapotranspiration. Analysis of subsurface hydraulic gradients, water-table fluctuations, and streamflow variability indicates a strong linkage between groundwater and wetlands, lakes and streams. Gradient analysis indicates that most wetlands are in groundwater discharge areas, but some wetlands are in groundwater recharge areas. The depth to the water table ranges from zero at surface-water features up to about 10 meters in topographically high areas. Depth to water fluctuates seasonally, and the magnitude of these fluctuations generally increases with distance from surface water. Variations in the permeability of the soils and sediments of the aquifer system strongly affect patterns of water movement through the subsurface and the interaction of groundwater with wetlands, lakes and streams. Mean annual streamflow during 2004-06 ranged from 83 to 106 percent of the long-term mean annual discharge, indicating that the data-collection period can be considered representative of average conditions. Measurements of groundwater levels, stream stage, and stream discharge and locations of start-of-flow are illustrated in basin-wide maps of water-table altitude, depth to the water table, and stream base flow during the period. Water-level data collected along 15 hydrologic transects that span the range of environments from uplands through wetlands to surface water were used to determine hydraulic gradients, potential flow directions, and areas of recharge and discharge. These data provide information about the localized interactions of groundwater with wetlands and surface water. Wetlands were categorized with r

Citizen science contributes to our knowledge of invasive plant species distributions

USGS Publications Warehouse

Crall, Alycia W.; Jarnevich, Catherine S.; Young, Nicholas E.; Panke, Brendon; Renz, Mark; Stohlgren, Thomas

2015-01-01

Citizen science is commonly cited as an effective approach to expand the scale of invasive species data collection and monitoring. However, researchers often hesitate to use these data due to concerns over data quality. In light of recent research on the quality of data collected by volunteers, we aimed to demonstrate the extent to which citizen science data can increase sampling coverage, fill gaps in species distributions, and improve habitat suitability models compared to professionally generated data sets used in isolation. We combined data sets from professionals and volunteers for five invasive plant species (Alliaria petiolata, Berberis thunbergii, Cirsium palustre, Pastinaca sativa, Polygonum cuspidatum) in portions of Wisconsin. Volunteers sampled counties not sampled by professionals for three of the five species. Volunteers also added presence locations within counties not included in professional data sets, especially in southern portions of the state where professional monitoring activities had been minimal. Volunteers made a significant contribution to the known distribution, environmental gradients sampled, and the habitat suitability of P. cuspidatum. Models generated with professional data sets for the other four species performed reasonably well according to AUC values (>0.76). The addition of volunteer data did not greatly change model performance (AUC > 0.79) but did change the suitability surface generated by the models, making them more realistic. Our findings underscore the need to merge data from multiple sources to improve knowledge of current species distributions, and to predict their movement under present and future environmental conditions. The efficiency and success of these approaches require that monitoring efforts involve multiple stakeholders in continuous collaboration via established monitoring networks.

Microfabricated gas chromatograph for on-site determinations of TCE in indoor air arising from vapor intrusion. 2. Spatial/temporal monitoring.

PubMed

Kim, Sun Kyu; Burris, David R; Bryant-Genevier, Jonathan; Gorder, Kyle A; Dettenmaier, Erik M; Zellers, Edward T

2012-06-05

We demonstrate the use of two prototype Si-microfabricated gas chromatographs (μGC) for continuous, short-term measurements of indoor trichloroethylene (TCE) vapor concentrations related to the investigation of TCE vapor intrusion (VI) in two houses. In the first house, with documented TCE VI, temporal variations in TCE air concentrations were monitored continuously for up to 48 h near the primary VI entry location under different levels of induced differential pressure (relative to the subslab). Concentrations ranged from 0.23 to 27 ppb by volume (1.2-150 μg/m(3)), and concentration trends agreed closely with those determined from concurrent reference samples. The sensitivity and temporal resolution of the measurements were sufficiently high to detect transient fluctuations in concentration resulting from short-term changes in variables affecting the extent of VI. Spatial monitoring showed a decreasing TCE concentration gradient with increasing distance from the primary VI entry location. In the second house, with no TCE VI, spatial profiles derived from the μGC prototype data revealed an intentionally hidden source of TCE within a closet, demonstrating the capability for locating non-VI sources. Concentrations measured in this house ranged from 0.51 to 56 ppb (2.7-300 μg/m(3)), in good agreement with reference method values. This first field demonstration of μGC technology for automated, near-real-time, selective VOC monitoring at low- or subppb levels augurs well for its use in short- and long-term on-site analysis of indoor air in support of VI assessments.

How Many Grid Points are Required for Time Accurate Simulations Scheme Selection and Scale-Discriminant Stabilization

DTIC Science & Technology

2015-11-24

spatial concerns: ¤ how well are gradients captured? (resolution requirement) spatial/temporal concerns: ¤ dispersion and dissipation error...distribution is unlimited. Gradient Capture vs. Resolution: Single Mode FFT: Solution/Derivative: Convergence: f x( )= sin(x) with x∈[0,2π ] df dx...distribution is unlimited. Gradient Capture vs. Resolution: 
 Multiple Modes FFT: Solution/Derivative: Convergence: 6 __ CD02 __ CD04 __ CD06

Intracellular dynamics during directional sensing of chemotactic cells

NASA Astrophysics Data System (ADS)

Amselem, Gabriel; Bodenschatz, Eberhard; Beta, Carsten

2007-03-01

We use an experimental approach based on the photo-chemical release of signaling molecules in microfluidic environments to expose chemotactic cells to well controlled chemoattractant stimuli. We apply this technique to study intracellular translocation of fluorescently labeled PH-domain proteins in the social ameba Dictyostelium discoideum. Single chemotactic Dictyostelium cells are exposed to localized, well defined gradients in the chemoattractant cAMP and their translocation response is quantified as a function of the external gradient.

Application of an online ion-chromatography-based instrument for gradient flux measurements of speciated nitrogen and sulfur

NASA Astrophysics Data System (ADS)

Rumsey, Ian C.; Walker, John T.

2016-06-01

The dry component of total nitrogen and sulfur atmospheric deposition remains uncertain. The lack of measurements of sufficient chemical speciation and temporal extent make it difficult to develop accurate mass budgets and sufficient process level detail is not available to improve current air-surface exchange models. Over the past decade, significant advances have been made in the development of continuous air sampling measurement techniques, resulting with instruments of sufficient sensitivity and temporal resolution to directly quantify air-surface exchange of nitrogen and sulfur compounds. However, their applicability is generally restricted to only one or a few of the compounds within the deposition budget. Here, the performance of the Monitor for AeRosols and GAses in ambient air (MARGA 2S), a commercially available online ion-chromatography-based analyzer is characterized for the first time as applied for air-surface exchange measurements of HNO3, NH3, NH4+, NO3-, SO2 and SO42-. Analytical accuracy and precision are assessed under field conditions. Chemical concentrations gradient precision are determined at the same sampling site. Flux uncertainty measured by the aerodynamic gradient method is determined for a representative 3-week period in fall 2012 over a grass field. Analytical precision and chemical concentration gradient precision were found to compare favorably in comparison to previous studies. During the 3-week period, percentages of hourly chemical concentration gradients greater than the corresponding chemical concentration gradient detection limit were 86, 42, 82, 73, 74 and 69 % for NH3, NH4+, HNO3, NO3-, SO2 and SO42-, respectively. As expected, percentages were lowest for aerosol species, owing to their relatively low deposition velocities and correspondingly smaller gradients relative to gas phase species. Relative hourly median flux uncertainties were 31, 121, 42, 43, 67 and 56 % for NH3, NH4+, HNO3, NO3-, SO2 and SO42-, respectively. Flux uncertainty is dominated by uncertainty in the chemical concentrations gradients during the day but uncertainty in the chemical concentration gradients and transfer velocity are of the same order at night. Results show the instrument is sufficiently precise for flux gradient applications.

Using transcriptomic tools to evaluate biological effects across effluent gradients at a diverse set of study sites in Minnesota, USA

EPA Science Inventory

The aim of this overall project was to explore the utility of ?‘omics’ approaches in monitoring aquatic environments where complex, often unknown, stressors make chemical-specific risk assessment untenable. This specific component of the effort examined changes in the fathead min...

Satellite-based monitoring of cyanobacteria blooms from 2002–2011 for 11 reservoirs with watersheds along an agricultural gradient

EPA Science Inventory

Imagery acquired by the Envisat Medium Resolution Imaging Spectrometer from 2002-2011 was used to estimate cyanobacteria cell densities for 11 reservoirs in Indiana, Ohio, and Kentucky, USA (surface areas 8–43 km2; 864 total images spanning May–September). This initia...

Proposed Drill Sites

DOE Data Explorer

Lane, Michael

2013-06-28

Proposed drill sites for intermediate depth temperature gradient holes and/or deep resource confirmation wells. Temperature gradient contours based on shallow TG program and faults interpreted from seismic reflection survey are shown, as are two faults interpreted by seismic contractor Optim but not by Oski Energy, LLC.

Hydrologic response across a snow persistence gradient on the west and east slopes of the Rocky Mountains in Colorado

NASA Astrophysics Data System (ADS)

Richard, G. A.; Hammond, J. C.; Kampf, S. K.; Moore, C. D.; Eurich, A.

2017-12-01

Snowpack trend analyses and modeling studies suggest that lower elevation snowpacks in mountain regions are most sensitive to drought and warming temperatures, however, in Colorado, most snow monitoring occurs in the high elevations where snow lasts throughout the winter and most streamflow monitoring occurs at lower elevations. The lack of combined snow and streamflow monitoring in watersheds along the transition from intermittent to persistent snow creates a gap in our understanding of snowmelt and runoff within the intermittent-persistent snow transition. Expanded hydrologic monitoring that spans the gradient of snow conditions in Colorado can help improve streamflow prediction and inform land and water managers. This study established hydrologic monitoring watersheds in intermittent, transitional, and persistent snow zones on the east slope and west slope of the Rocky Mountains in Colorado, and uses this monitoring network to improve understanding of how snow accumulation and melt affect soil moisture and streamflow generation under different snow conditions. We monitored six small watersheds (three west slope, three east slope) (0.8 to 3.9 km2) that drain intermittent, transitional, and persistent snow zones. At each site, we measured: streamflow, snow depth, soil moisture, precipitation, air temperature, and snow water equivalent (SWE). In our first season of monitoring, the west slope persistent and transitional sites had more mid-winter melt and infiltration, shorter snowpack duration, and lower peak SWE than the east slope sites. Snow cover remained at the east slope persistent site into June, whereas much of the snow at the persistent site on the west slope had already melted by early June. The difference in soil water input likely has consequences for streamflow response that we will continue to examine in future years. At the west slope intermittent site, the stream did not flow during the entire first year of monitoring, while at the east slope intermittent site, the streams flowed intermittently during winter and spring, likely a result of different subsurface geology. With our ongoing watershed monitoring across a broad range of snow conditions in Colorado, we continue to learn about the factors that increase or decrease streamflow in the headwater streams that supply the state's major rivers.

Analysis of the geochemical gradient created by surface-groundwater interactions within riverbanks of the East River in Crested Butte, Colorado

NASA Astrophysics Data System (ADS)

Lunzer, J.; Williams, K. H.; Malenda, H. F.; Nararne-Sitchler, A.

2016-12-01

An improved understanding of the geochemical gradient created by the mixing of surface and groundwater of a river system will have considerable impact on our understanding of microorganisms, organic cycling and biogeochemical processes within these zones. In this study, the geochemical gradient in the hyporheic zone is described using a variety of geochemical properties. A system of shallow groundwater wells were installed in a series of transects along a stream bank. Each transect consists of several wells that progress away from the river bank in a perpendicular fashion. From these wells, temperature, conductivity and pH of water samples were obtained via hand pumping or bailing. These data show a clear geochemical gradient that displays a distinct zone in the subsurface where the geochemical conditions change from surface water dominated to groundwater dominated. For this study, the East River near Crested Butte, Colorado has been selected as the river of interest due the river being a relatively undisturbed floodplain. Additionally, the specific section chosen on the East River displays relatively high sinuosity meaning that these meandering sections will produce hyporheic zones that are more laterally expansive than what would be expected on a river of lower sinuosity. This increase in lateral extension of the hyporheic zone will make depicting the subtle changes in the geochemical gradient much easier than that of a river system in which the hyporheic zone is not as laterally extensive. Data has been and will be continued to be collected at different river discharges to evaluate the geochemical gradient at differing rates. Overall, this characterization of the geochemical gradient along stream banks will produce results that will aid in the further use of geochemical methods to classify and understand hyporheic exchange zones and the potential expansion of these techniques to river systems of differing geologic and geographic conditions.

Gyrokinetic GDC turbulence simulations: confirming a new instability regime in LAPD plasmas

NASA Astrophysics Data System (ADS)

Pueschel, M. J.; Rossi, G.; Told, D.; Terry, P. W.; Jenko, F.; Carter, T. A.

2016-10-01

Recent high-beta experiments at the LArge Plasma Device have found significant parallel magnetic fluctuations in the region of large pressure gradients. Linear gyrokinetic simulations show the dominant instability at these radii to be the gradient-driven drift coupling (GDC) mode, a non-textbook mode driven by pressure gradients and destabilized by the coupling of ExB and grad-B∥ drifts. Unlike in previous studies, the large parallel extent of the device allows for finite-kz versions of this instability in addition to kz = 0 . The locations of maximum linear growth match very well with experimentally observed peaks of B∥ fluctuations. Local nonlinear simulations reproduce many features of the observations fairly well, with the exception of Bperp fluctuations, for which experimental profiles suggest a source unrelated to pressure gradients. In toto, the results presented here show that turbulence and transport in these experiments are driven by the GDC instability, that important characteristics of the linear instability carry over to nonlinear simulations, and - in the context of validation - that the gyrokinetic framework performs surprisingly well far outside its typical area of application, increasing confidence in its predictive abilities. Supported by U.S. DOE.

How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?

PubMed

Santos, M V; Sansinena, M; Zaritzky, N; Chirife, J

2013-01-01

The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 degree C. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen/extender packaged in straw was extended to predict internal temperature gradients during the cooling/freezing process. Results showed maximum temperature differences between the centre and the periphery of semen/extender "liquid" column was 1.5 degree C for an external heat transfer coefficient, h = 15 W per (m(2) K), and only 0.5 degree C for h = 5 W per (m(2) K). It is concluded that if a thermocouple wire were inserted in a 0.5 ml plastic straw to monitor the freezing process in nitrogen vapor, its radial position would have little importance since expected internal gradients may be safely neglected. This finding facilitates the interpretation of freezing rates in 0.5 ml plastic straws immersed in nitrogen vapor over liquid nitrogen, a widely used method for cryopreservation of bovine spermatozoa.

Application of Unmanned Aerial System-based Photogrammetry to Monitor Landforms Evolution of Mudstone Badlands

NASA Astrophysics Data System (ADS)

Chen, Yichin

2017-04-01

Mudstone badlands are the area characteristized by its rapid erosion and steep, fractured, and barren landforms. Monitoring the topography changes in badland help improve our knowledge of the hillslope and river processing on landforms and develop susceptibility model for surface erosion hazards. Recently, advances in unmanned aerial system (UAS) and close-range photogrammetry technology have opened up the possibility of effectively measuring topography changes with high spatiotemporal resolutions. In this study, we used the UAS and close-range photogrammetry technology to monitor the topography changes in a rapidly eroded badland, south-western Taiwan. A small mudstone hillslope with area of 0.2 ha approximately and with slope gradient of 37 degrees was selected as the study site. A widely used and commercial quadcopter equipped non-metric camera was used to take images with ground sampling distance (GSD) 5 mm approximately. The Pix4DMapper, a commercial close-range photogrammetry software, was used to perform stereo matching, extract point clouds, generate digital surface models (DSMs) and orthoimage. To control model accuracy, a set of ground control points was surveyed by using eGPS. The monitoring was carried out after every significant rainfall event that may induced observable erosion in the badland site. The results show that DSMs have the GSDs of 4.0 5.4 mm and vertical accuracy of 61 116 mm. The accuracy largely depends on the quality of ground control points. The spatial averaged erosion rate during six months of monitoring was 328 mm, which is higher in the gully sides than in the ridges. The erosion rate is positively correlated with the slope gradient and drainage contributing area that implies the important role of surface gully erosion in mudstone badland erosion. This study shows that UAS and close-range photogrammetry technology can be used to monitor the topography change in badland areas effectively and can provide high spatiotemporal resolutions of DSMs for developing distributed surface erosion models.

Design and fabrication of a metamaterial gradient index diffraction grating at infrared wavelengths.

PubMed

Tsai, Yu-Ju; Larouche, Stéphane; Tyler, Talmage; Lipworth, Guy; Jokerst, Nan M; Smith, David R

2011-11-21

We demonstrate the design, fabrication and characterization of an artificially structured, gradient index metamaterial with a linear index variation of Δn ~ 3.0. The linear gradient profile is repeated periodically to form the equivalent of a blazed grating, with the gradient occurring across a spatial distance of 61 μm. The grating, which operates at a wavelength of 10.6 μm, is composed of non-resonant, progressively modified "I-beam" metamaterial elements and approximates a linear phase shift gradient using 61 distinguishable phase levels. The grating structure consists of four layers of lithographically patterned metallic I-beam elements separated by dielectric layers of SiO(2). The index gradient is confirmed by comparing the measured magnitudes of the -1, 0 and +1 diffracted orders to those obtained from full wave simulations incorporating all material properties of the metals and dielectrics of the structures. The large index gradient has the potential to enable compact infrared diffractive and gradient index optics, as well as more exotic transformation optical media. © 2011 Optical Society of America

The geothermal gradient map of Central Tunisia: Comparison with structural, gravimetric and petroleum data

NASA Astrophysics Data System (ADS)

Dhia, Hamed Ben

1987-10-01

Five hundred and fifty temperature values, initially measured as either bottom-hole temperatures (BHT) or drill-stem tests (DST), from 98 selected petroleum exploration wells form the basis of a geothermal gradient map of central Tunisia. A "global-statistical" method was employed to correct the BHT measurements, using the DST as references. The geothermal gradient ranges from 23° to 49°C/km. Comparison of the geothermal gradient with structural, gravimetric and petroleum data indicates that: (1) the general trend of the geothermal gradient curves reflects the main structural directions of the region, (2) zones of low and high geothermal gradient are correlated with zones of negative and positive Bouguer anomalies and (3) the five most important oil fields of central Tunisia are located near the geothermal gradient curve of 40° C/km. Such associations could have practical importance in petroleum exploration, but their significance must first be established through further investigation and additional data.

Plasma density irregularities and Total Electron Content gradients over Europe

NASA Astrophysics Data System (ADS)

Zakharenkova, I.; Kotulak, K.; Cherniak, I.; Krankowski, A.; Froń, A.

2017-12-01

Perturbations of the ionospheric plasma density are crucial from the scientific and application points of view, as they can severe affect radio signals used in the Global Navigation Satellite Systems (GNSS) and low frequency radio astronomy. For several decades the ionospheric irregularities have been extensively studied by different techniques, including ground-based GNSS observations. Spatial distribution of ionospheric disturbances can be specified by horizontal gradients of the ionospheric density (total electron content, TEC). Another, widely used tool in irregularities monitoring is the rate of TEC index (ROTI). Recently, the Northern Hemisphere ROTI product has been implemented to the International GNSS Service (IGS) and available for community. In this study, we present climatology of the spatial TEC gradients occurred over European region at high to middle latitudes. We developed the TEC gradient maps based on the high-resolution (0.5 degree in latitude and longitude) regional TEC maps covering Europe. The obtained climatological characteristics of the spatial TEC gradients are superimposed and analyzed with the global and regional ROTI product in order to reveal development of highly intense plasma irregularities occurred at high and middle latitudes. During geomagnetic storm the complex of physical processes at auroal zone leads to development of intnse ionospheric irregularities and travelling ionospheric disturbances (TIDs). We presents results of the geomagnetic storm analysis including the 2013 and 2015 St. Patrick's Day geomagnetic storms.

Contaminant Gradients in Trees: Directional Tree Coring Reveals Boundaries of Soil and Soil-Gas Contamination with Potential Applications in Vapor Intrusion Assessment.

PubMed

Wilson, Jordan L; Samaranayake, V A; Limmer, Matthew A; Schumacher, John G; Burken, Joel G

2017-12-19

Contaminated sites pose ecological and human-health risks through exposure to contaminated soil and groundwater. Whereas we can readily locate, monitor, and track contaminants in groundwater, it is harder to perform these tasks in the vadose zone. In this study, tree-core samples were collected at a Superfund site to determine if the sample-collection location around a particular tree could reveal the subsurface location, or direction, of soil and soil-gas contaminant plumes. Contaminant-centroid vectors were calculated from tree-core data to reveal contaminant distributions in directional tree samples at a higher resolution, and vectors were correlated with soil-gas characterization collected using conventional methods. Results clearly demonstrated that directional tree coring around tree trunks can indicate gradients in soil and soil-gas contaminant plumes, and the strength of the correlations were directly proportionate to the magnitude of tree-core concentration gradients (spearman's coefficient of -0.61 and -0.55 in soil and tree-core gradients, respectively). Linear regression indicates agreement between the concentration-centroid vectors is significantly affected by in planta and soil concentration gradients and when concentration centroids in soil are closer to trees. Given the existing link between soil-gas and vapor intrusion, this study also indicates that directional tree coring might be applicable in vapor intrusion assessment.

Contaminant gradients in trees: Directional tree coring reveals boundaries of soil and soil-gas contamination with potential applications in vapor intrusion assessment

USGS Publications Warehouse

Wilson, Jordan L.; Samaranayake, V.A.; Limmer, Matthew A.; Schumacher, John G.; Burken, Joel G.

2017-01-01

Contaminated sites pose ecological and human-health risks through exposure to contaminated soil and groundwater. Whereas we can readily locate, monitor, and track contaminants in groundwater, it is harder to perform these tasks in the vadose zone. In this study, tree-core samples were collected at a Superfund site to determine if the sample-collection location around a particular tree could reveal the subsurface location, or direction, of soil and soil-gas contaminant plumes. Contaminant-centroid vectors were calculated from tree-core data to reveal contaminant distributions in directional tree samples at a higher resolution, and vectors were correlated with soil-gas characterization collected using conventional methods. Results clearly demonstrated that directional tree coring around tree trunks can indicate gradients in soil and soil-gas contaminant plumes, and the strength of the correlations were directly proportionate to the magnitude of tree-core concentration gradients (spearman’s coefficient of -0.61 and -0.55 in soil and tree-core gradients, respectively). Linear regression indicates agreement between the concentration-centroid vectors is significantly affected by in-planta and soil concentration gradients and when concentration centroids in soil are closer to trees. Given the existing link between soil-gas and vapor intrusion, this study also indicates that directional tree coring might be applicable in vapor intrusion assessment.

The Society of Thoracic Surgeons, The Society of Cardiovascular Anesthesiologists, and The American Society of ExtraCorporeal Technology: Clinical Practice Guidelines for Cardiopulmonary Bypass--Temperature Management during Cardiopulmonary Bypass.

PubMed

Engelman, Richard; Baker, Robert A; Likosky, Donald S; Grigore, Alina; Dickinson, Timothy A; Shore-Lesserson, Linda; Hammon, John W

2015-09-01

To improve our understanding of the evidence-based literature supporting temperature management during adult cardiopulmonary bypass, The Society of Thoracic Surgeons, the Society of Cardiovascular Anesthesiology and the American Society of ExtraCorporeal Technology tasked the authors to conduct a review of the peer-reviewed literature, including 1) optimal site for temperature monitoring, 2) avoidance of hyperthermia, 3) peak cooling temperature gradient and cooling rate, and 4) peak warming temperature gradient and rewarming rate. Authors adopted the American College of Cardiology/American Heart Association method for development clinical practice guidelines, and arrived at the following recommendation.

Population structure and abundance of arsenite-oxidizing bacteria along an arsenic pollution gradient in waters of the upper isle River Basin, France.

PubMed

Quéméneur, Marianne; Cébron, Aurélie; Billard, Patrick; Battaglia-Brunet, Fabienne; Garrido, Francis; Leyval, Corinne; Joulian, Catherine

2010-07-01

Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qPCR) were successfully developed to monitor functional aoxB genes as markers of aerobic arsenite oxidizers. DGGE profiles showed a shift in the structure of the aoxB-carrying bacterial population, composed of members of the Alpha-, Beta- and Gammaproteobacteria, depending on arsenic (As) and E(h) levels in Upper Isle River Basin waters. The highest aoxB gene densities were found in the most As-polluted oxic surface waters but without any significant correlation with environmental factors. Arsenite oxidizers seem to play a key role in As mobility in As-impacted waters.

The molecular gradient using the divide-expand-consolidate resolution of the identity second-order Møller-Plesset perturbation theory: The DEC-RI-MP2 gradient

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bykov, Dmytro; Kristensen, Kasper; Kjærgaard, Thomas

We report an implementation of the molecular gradient using the divide-expand-consolidate resolution of the identity second-order Møller-Plesset perturbation theory (DEC-RI-MP2). The new DEC-RI-MP2 gradient method combines the precision control as well as the linear-scaling and massively parallel features of the DEC scheme with efficient evaluations of the gradient contributions using the RI approximation. We further demonstrate that the DEC-RI-MP2 gradient method is capable of calculating molecular gradients for very large molecular systems. A test set of supramolecular complexes containing up to 158 atoms and 1960 contracted basis functions has been employed to demonstrate the general applicability of the DEC-RI-MP2 methodmore » and to analyze the errors of the DEC approximation. Moreover, the test set contains molecules of complicated electronic structures and is thus deliberately chosen to stress test the DEC-RI-MP2 gradient implementation. Additionally, as a showcase example the full molecular gradient for insulin (787 atoms and 7604 contracted basis functions) has been evaluated.« less

Microbial response to environmental gradients in a ceramic-based diffusion system.

PubMed

Wolfaardt, G M; Hendry, M J; Birkham, T; Bressel, A; Gardner, M N; Sousa, A J; Korber, D R; Pilaski, M

2008-05-01

A solid, porous matrix was used to establish steady-state concentration profiles upon which microbial responses to concentration gradients of nutrients or antimicrobial agents could be quantified. This technique relies on the development of spatially defined concentration gradients across a ceramic plate resulting from the diffusion of solutes through the porous ceramic matrix. A two-dimensional, finite-element numerical transport model was used to predict the establishment of concentration profiles, after which concentration profiles of conservative tracers were quantified fluorometrically and chemically at the solid-liquid interface to verify the simulated profiles. Microbial growth responses to nutrient, hypochloride, and antimicrobial concentration gradients were then quantified using epifluorescent or scanning confocal laser microscopy. The observed microbial response verified the establishment and maintenance of stable concentration gradients along the solid-liquid interface. These results indicate the ceramic diffusion system has potential for the isolation of heterogeneous microbial communities as well as for testing the efficacy of antimicrobial agents. In addition, the durability of the solid matrix allowed long-term investigations, making this approach preferable to conventional gel-stabilized systems that are impeded by erosion as well as expansion or shrinkage of the gel. Copyright 2008 Wiley Periodicals, Inc.

Effluent migration from septic tank systems in two different lithologies, Broward County, Florida

USGS Publications Warehouse

Waller, B.G.; Howie, Barbara; Causaras, C.R.

1987-01-01

Two septic tank test sites, one in sand and one in limestone, in Broward County, Florida, were analyzed for effluent migration. Groundwater from shallow wells, both in background areas and hydraulically down-gradient of the septic tank system, was sampled during a 16-month period from April 1983 through August 1984. Water quality indicators were used to determine the effluent affected zone near the septic tank systems. Specific conductance levels and concentrations of chloride, sulfate, ammonium, and nitrate indicated effluent movement primarily in a vertical direction with abrupt dilution as it moved down-gradient. Effluent was detected in the sand to a depth more than 20 ft below the septic tank outlet, but was diluted to near background conditions 50 ft down-gradient from the tank. Effluent in the limestone was detected in all three observation wells to depths exceeding 25 ft below the septic tank outlet and was diluted, but still detectable, 40 ft down-gradient. The primary controls on effluent movement from septic tank systems in Broward County are the lithology and layering of the geologic materials, hydraulic gradients, and the volume and type of use the system receives. (Author 's abstract)

Application of denaturing high-performance liquid chromatography for monitoring sulfate-reducing bacteria in oil fields.

PubMed

Priha, Outi; Nyyssönen, Mari; Bomberg, Malin; Laitila, Arja; Simell, Jaakko; Kapanen, Anu; Juvonen, Riikka

2013-09-01

Sulfate-reducing bacteria (SRB) participate in microbially induced corrosion (MIC) of equipment and H2S-driven reservoir souring in oil field sites. Successful management of industrial processes requires methods that allow robust monitoring of microbial communities. This study investigated the applicability of denaturing high-performance liquid chromatography (DHPLC) targeting the dissimilatory sulfite reductase ß-subunit (dsrB) gene for monitoring SRB communities in oil field samples from the North Sea, the United States, and Brazil. Fifteen of the 28 screened samples gave a positive result in real-time PCR assays, containing 9 × 10(1) to 6 × 10(5) dsrB gene copies ml(-1). DHPLC and denaturing gradient gel electrophoresis (DGGE) community profiles of the PCR-positive samples shared an overall similarity; both methods revealed the same samples to have the lowest and highest diversity. The SRB communities were diverse, and different dsrB compositions were detected at different geographical locations. The identified dsrB gene sequences belonged to several phylogenetic groups, such as Desulfovibrio, Desulfococcus, Desulfomicrobium, Desulfobulbus, Desulfotignum, Desulfonatronovibrio, and Desulfonauticus. DHPLC showed an advantage over DGGE in that the community profiles were very reproducible from run to run, and the resolved gene fragments could be collected using an automated fraction collector and sequenced without a further purification step. DGGE, on the other hand, included casting of gradient gels, and several rounds of rerunning, excising, and reamplification of bands were needed for successful sequencing. In summary, DHPLC proved to be a suitable tool for routine monitoring of the diversity of SRB communities in oil field samples.

Benthic Foraminifera As A Novel Bio-monitoring Tool In The Assessment Of Environmental Impacts Linked To Marine Aquaculture

NASA Astrophysics Data System (ADS)

Alammar, Montaha; Austin, William

2017-04-01

The present study represents an attempt to evaluate the impacts of marine aquaculture on benthic foraminiferal communities in order to develop an improved, quantitative understanding of their response to the variation in benthic environmental gradients associated with fish farms in Scotland. Furthermore, their performance as a bio-monitoring tool will be discussed and outlined in ongoing research to evaluate their performance alongside traditional bioecological indicators. Foraminiferal faunas offer the potential to assess ecological quality status through their response to stress gradients (e.g. organic matter enrichment), such as that caused by intensive fish farming in coastal sediments. In this study, we followed the Foraminiferal Bio-Monitoring (FOBIMO) protocol (Schönfeld. et al., 2012), which proposed a standardised methodology of using foraminifera as a bio-monitoring tool to evaluate the quality of the marine ecosystem and applied these protocols to the rapidly expanding marine aquaculture sector in Scotland, UK. Eight stations were sampled along a transect in Loch Creran, west coast of Scotland, to describe the spatial and down-core (temporal) distribution pattern of benthic foraminiferal assemblages. Triplicate, Rose-Bengal stained samples from an interval of (0-1cm) below the sediment surface were studied at each station from below the fish cages (impacted stations) to a distance from the farming sites (control stations). Morphospecies counts were conducted, and the organic carbon and the grain size distributions determined. Species richness beneath these fish farming cages were analysed and showed a reduction of foraminifera density and diversity at the impacted stations.

Gradient Material Strategies for Hydrogel Optimization in Tissue Engineering Applications

PubMed Central

2018-01-01

Although a number of combinatorial/high-throughput approaches have been developed for biomaterial hydrogel optimization, a gradient sample approach is particularly well suited to identify hydrogel property thresholds that alter cellular behavior in response to interacting with the hydrogel due to reduced variation in material preparation and the ability to screen biological response over a range instead of discrete samples each containing only one condition. This review highlights recent work on cell–hydrogel interactions using a gradient material sample approach. Fabrication strategies for composition, material and mechanical property, and bioactive signaling gradient hydrogels that can be used to examine cell–hydrogel interactions will be discussed. The effects of gradients in hydrogel samples on cellular adhesion, migration, proliferation, and differentiation will then be examined, providing an assessment of the current state of the field and the potential of wider use of the gradient sample approach to accelerate our understanding of matrices on cellular behavior. PMID:29485612

Comparing Measures of Estuarine Ecosystem Production in a ...

EPA Pesticide Factsheets

Anthropogenic nutrient enrichments and concerted efforts at nutrient reductions, compounded with the influences of climate change, are likely changing the net ecosystem production (NEP) of our coastal systems. To quantify these changes, scientists monitor a range of physical, chemical, and biological parameters sampled at various frequencies. Water column chlorophyll concentrations are arguably the most commonly used indicator of net phytoplankton production, as well as a coarse indicator of NEP. We compared parameters that estimate production, including chlorophyll, across an experimental nutrient gradient and in situ in both well-mixed and stratified estuarine environments. Data from an experiment conducted in the early 1980s in mesocosms designed to replicate a well-mixed mid-Narragansett Bay (Rhode Island) water column were used to correlate changes in chlorophyll concentrations, pH, dissolved oxygen (O2), dissolved inorganic nitrogen, phosphate, and silicate concentrations, cell counts, and 14C carbon uptake measurements across a range of nutrient enrichments. The pH, O2, nutrient, and cell count measurements reflected seasonal cycles of spring blooms followed by late summer/early fall respiration periods across nutrient enrichments. Chlorophyll concentrations were more variable and rates of 14C productivity were inconsistent with observed trends in nutrient concentrations, pH, and O2 concentrations. Similar comparisons were made using data from a well-mixe

Continuous gradient scaffolds for rapid screening of cell-material interactions and interfacial tissue regeneration

PubMed Central

Bailey, Brennan M.; Nail, Lindsay N.; Grunlan, Melissa A.

2013-01-01

In tissue engineering, the physical and chemical properties of the scaffold mediates cell behavior including regeneration. Thus, a strategy that permits rapid screening of cell-scaffold interactions is critical. Herein, we have prepared eight “hybrid” hydrogel scaffolds in the form of continuous gradients such that a single scaffold contains spatially varied properties. These scaffolds are based on combining an inorganic macromer [methacrylated star polydimethylsiloxane, PDMSstar-MA] and organic macromer [poly(ethylene glycol)diacrylate, PEG-DA] as well both aqueous and organic fabrication solvents. Having previously demonstrated its bioactivity and osteoinductivity, PDMSstar-MA is a particularly powerful component to incorporate into instructive gradient scaffolds based on PEG-DA. The following parameters were varied to produce the different gradients or gradual transitions in: (1) the wt% ratio of PDMSstar-MA to PEG-DA macromers, (2) the total wt% macromer concentration, (3) the number average molecular weight (Mn) of PEG-DA and (4) the Mn of PDMSstar-MA. Upon dividing each scaffold into four “zones” perpendicular to the gradient, we were able to demonstrate the spatial variation in morphology, bioactivity, swelling and modulus. Among these gradient scaffolds are those in which swelling and modulus are conveniently decoupled. In addition to rapid screening of cell-material interactions, these scaffolds are well-suited for regeneration of interfacial tissues (e.g. osteochondral tissues) that transition from one tissue type to another. PMID:23707502

Tuning the Voices of a Choir: Detecting Ecological Gradients in Time-Series Populations.

PubMed

Buras, Allan; van der Maaten-Theunissen, Marieke; van der Maaten, Ernst; Ahlgrimm, Svenja; Hermann, Philipp; Simard, Sonia; Heinrich, Ingo; Helle, Gerd; Unterseher, Martin; Schnittler, Martin; Eusemann, Pascal; Wilmking, Martin

2016-01-01

This paper introduces a new approach-the Principal Component Gradient Analysis (PCGA)-to detect ecological gradients in time-series populations, i.e. several time-series originating from different individuals of a population. Detection of ecological gradients is of particular importance when dealing with time-series from heterogeneous populations which express differing trends. PCGA makes use of polar coordinates of loadings from the first two axes obtained by principal component analysis (PCA) to define groups of similar trends. Based on the mean inter-series correlation (rbar) the gain of increasing a common underlying signal by PCGA groups is quantified using Monte Carlo Simulations. In terms of validation PCGA is compared to three other existing approaches. Focusing on dendrochronological examples, PCGA is shown to correctly determine population gradients and in particular cases to be advantageous over other considered methods. Furthermore, PCGA groups in each example allowed for enhancing the strength of a common underlying signal and comparably well as hierarchical cluster analysis. Our results indicate that PCGA potentially allows for a better understanding of mechanisms causing time-series population gradients as well as objectively enhancing the performance of climate transfer functions in dendroclimatology. While our examples highlight the relevance of PCGA to the field of dendrochronology, we believe that also other disciplines working with data of comparable structure may benefit from PCGA.

Tuning the Voices of a Choir: Detecting Ecological Gradients in Time-Series Populations

PubMed Central

Buras, Allan; van der Maaten-Theunissen, Marieke; van der Maaten, Ernst; Ahlgrimm, Svenja; Hermann, Philipp; Simard, Sonia; Heinrich, Ingo; Helle, Gerd; Unterseher, Martin; Schnittler, Martin; Eusemann, Pascal; Wilmking, Martin

2016-01-01

This paper introduces a new approach–the Principal Component Gradient Analysis (PCGA)–to detect ecological gradients in time-series populations, i.e. several time-series originating from different individuals of a population. Detection of ecological gradients is of particular importance when dealing with time-series from heterogeneous populations which express differing trends. PCGA makes use of polar coordinates of loadings from the first two axes obtained by principal component analysis (PCA) to define groups of similar trends. Based on the mean inter-series correlation (rbar) the gain of increasing a common underlying signal by PCGA groups is quantified using Monte Carlo Simulations. In terms of validation PCGA is compared to three other existing approaches. Focusing on dendrochronological examples, PCGA is shown to correctly determine population gradients and in particular cases to be advantageous over other considered methods. Furthermore, PCGA groups in each example allowed for enhancing the strength of a common underlying signal and comparably well as hierarchical cluster analysis. Our results indicate that PCGA potentially allows for a better understanding of mechanisms causing time-series population gradients as well as objectively enhancing the performance of climate transfer functions in dendroclimatology. While our examples highlight the relevance of PCGA to the field of dendrochronology, we believe that also other disciplines working with data of comparable structure may benefit from PCGA. PMID:27467508

One-Dimension Diffusion Preparation of Concentration-Gradient Fe₂O₃/SiO₂ Aerogel.

PubMed

Zhang, Ting; Wang, Haoran; Zhou, Bin; Ji, Xiujie; Wang, Hongqiang; Du, Ai

2018-06-21

Concentration-gradient Fe₂O₃/SiO₂ aerogels were prepared by placing an MTMS (methyltrimethoxysilane)-derived SiO₂ aerogel on an iron gauze with an HCl atmosphere via one-dimensional diffusion, ammonia-atmosphere fixing, supercritical fluid drying and thermal treatment. The energy dispersive spectra show that the Fe/Si molar ratios change gradually from 2.14% to 18.48% with a height of 40 mm. Pore-size distribution results show that the average pore size of the sample decreases from 15.8 nm to 3.1 nm after diffusion. This corresponds well with TEM results, indicating a pore-filling effect of the Fe compound. In order to precisely control the gradient, diffusion kinetics are further studied by analyzing the influence of time and position on the concentration of the wet gel. At last, it is found that the diffusion process could be fitted well with the one-dimensional model of Fick’s second law, demonstrating the feasibility of the precise design and control of the concentration gradient.

Observations of a stratospheric depletion and annual mean interhemispheric gradient in the atmospheric Ar/N2 ratio from the HIPPO Global campaign

NASA Astrophysics Data System (ADS)

Bent, J. D.; Keeling, R. F.; Stephens, B. B.; Wofsy, S. C.; Daube, B. C.; Kort, E. A.; Pittman, J. V.; Jimenez-Pizarro, R.; Santoni, G.

2014-12-01

The atmospheric Ar/N2 ratio varies on a seasonal basis due to temperature-dependent solubility changes in the surface ocean. Low signal:noise ratios, limited vertical coverage, and sampler-sampler offsets have historically hampered characterization of vertical and inter-hemispheric gradients. We present data from the HIPPO Global campaign (2009-11) showing that Ar/N2 and interannually-detrended N2O correlate well in the lower stratosphere, suggesting that, as stratospheric air ages and loses N2O to photolysis and photo-oxidation, it also gradually loses argon to gravity as the heavier atom preferentially "rains out" of the air parcel. The HIPPO Ar/N2 data from the lower troposphere also resolve seasonal cycles in each hemisphere, as well as a gradient in the annual mean between hemispheres, with higher values in the southern hemisphere. The HIPPO cycles and inter-hemispheric gradient are in good agreement with data from surface stations.

The Galapagos archipelago: A natural laboratory to examine sharp hydroclimatic, geologic and anthropogenic gradients

USGS Publications Warehouse

Percy, Madelyn; Schmitt, Sarah; Riveros-Iregui, Diego; Mirus, Benjamin B.

2016-01-01

Poor understanding of the water cycle in tropical ecosystems has the potential to exacerbate water shortages and water crises in the region. We suggest that the Galápagos Islands provide an excellent proxy to regions across the tropics as a result of sharp hydroclimatic, anthropogenic, and pedohydrologic gradients across the archipelago. Hydroclimatic and pedohydrologic gradients are found across different elevations on single islands, as well as across the archipelago, whereas anthropogenic gradients reflect land use and land cover change across islands as population and growth in tourism have affected individual islands differently. This article highlights specific opportunities to further examine our understanding of the interactions between water and critical zone processes in tropical ecosystems, making connections between the Galápagos archipelago and much of the understudied tropics. The Galápagos archipelago offers a natural laboratory through which we can examine current threats to freshwater security as well as the dynamics of coupled natural and human systems.

A modified micrometeorological gradient method for estimating O3 dry depositions over a forest canopy

NASA Astrophysics Data System (ADS)

Wu, Z. Y.; Zhang, L.; Wang, X. M.; Munger, J. W.

2015-07-01

Small pollutant concentration gradients between levels above a plant canopy result in large uncertainties in estimated air-surface exchange fluxes when using existing micrometeorological gradient methods, including the aerodynamic gradient method (AGM) and the modified Bowen ratio method (MBR). A modified micrometeorological gradient method (MGM) is proposed in this study for estimating O3 dry deposition fluxes over a forest canopy using concentration gradients between a level above and a level below the canopy top, taking advantage of relatively large gradients between these levels due to significant pollutant uptake in the top layers of the canopy. The new method is compared with the AGM and MBR methods and is also evaluated using eddy-covariance (EC) flux measurements collected at the Harvard Forest Environmental Measurement Site, Massachusetts, during 1993-2000. All three gradient methods (AGM, MBR, and MGM) produced similar diurnal cycles of O3 dry deposition velocity (Vd(O3)) to the EC measurements, with the MGM method being the closest in magnitude to the EC measurements. The multi-year average Vd(O3) differed significantly between these methods, with the AGM, MBR, and MGM method being 2.28, 1.45, and 1.18 times that of the EC, respectively. Sensitivity experiments identified several input parameters for the MGM method as first-order parameters that affect the estimated Vd(O3). A 10% uncertainty in the wind speed attenuation coefficient or canopy displacement height can cause about 10% uncertainty in the estimated Vd(O3). An unrealistic leaf area density vertical profile can cause an uncertainty of a factor of 2.0 in the estimated Vd(O3). Other input parameters or formulas for stability functions only caused an uncertainly of a few percent. The new method provides an alternative approach to monitoring/estimating long-term deposition fluxes of similar pollutants over tall canopies.

The Trails of Superluminal Jet Components in 3C 111

NASA Technical Reports Server (NTRS)

Kadler, M.; Ros, E.; Perucho, M.; Kovalev, Y. Y.; Homan, D. C.; Agudo, I.; Kellermann, K. I.; Aller, M. F.; Aller, H. D.; Lister, M. L.;

Flume and field-based calibration of surrogate sensors for monitoring bedload transport

NASA Astrophysics Data System (ADS)

Mao, L.; Carrillo, R.; Escauriaza, C.; Iroume, A.

2016-01-01

Bedload transport assessment is important for geomorphological, engineering, and ecological studies of gravel-bed rivers. Bedload can be monitored at experimental stations that require expensive maintenance or by using portable traps, which allows measuring instantaneous transport rates but at a single point and at high costs and operational risks. The need for continuously measuring bedload intensity and dynamics has therefore increased the use and enhancement of surrogate methods. This paper reports on a set of flume experiments in which a Japanese acoustic pipe and an impact plate have been tested using four well-sorted and three poorly sorted sediment mixtures. Additional data were collected in a glacierized high-gradient Andean stream (Estero Morales) using a portable Bunte-type bedload sampler. Results show that the data provided by the acoustic pipe (which is amplified on 6 channels having different gains) can be calibrated for the grain size and for the intensity of transported sediments coarser than 9 mm (R2 = 0.93 and 0.88, respectively). Even if the flume-based calibration is very robust, upscaling the calibration to field applications is more challenging, and the bedload intensity could be predicted better than the grain size of transported sediments (R2 = 0.61 and 0.43, respectively). The inexpensive impact plate equipped with accelerometer could be calibrated for bedload intensity quite well in the flume but only poorly in the field (R2 = 0.16) and could not provide information on the size of transported sediments.

Impact of Elevated End-Diastolic Pulmonary Regurgitation Gradient on Worse Clinical Outcomes in Hospitalized Patients With Heart Failure.

PubMed

Honda, Yasuyuki; Nagai, Toshiyuki; Sugano, Yasuo; Honda, Satoshi; Okada, Atsushi; Asaumi, Yasuhide; Aiba, Takeshi; Noguchi, Teruo; Kusano, Kengo; Ogawa, Hisao; Yasuda, Satoshi; Anzai, Toshihisa

2017-02-15

The echo Doppler end-diastolic pulmonary regurgitation (EDPR) gradient correlates well with catheter-derived pulmonary artery diastolic pressure. An elevated EDPR gradient is associated with worse clinical outcomes in patients with stable coronary artery disease. However, the prognostic significance of EDPR gradient in patients with heart failure (HF) is unclear. The aim of the present study was to investigate the prognostic impact of EDPR gradient in HF. We retrospectively examined 751 consecutive hospitalized patients with acute HF. Those with acute coronary syndrome or in-hospital death and those without accessible EDPR gradient data at discharge were excluded. Finally, 265 patients were examined and divided into 2 groups according to EDPR gradient (cutoff 9 mm Hg). Adverse events were defined as worsening HF and death. Patients with elevated EDPR gradient had higher B-type natriuretic peptide, lower age, and lower left ventricular ejection fraction at discharge than those with nonelevated EDPR gradient. During a median follow-up of 429 days, elevated EDPR gradient was independently associated with adverse events (hazard ratio 2.34, 95% CI 1.44 to 3.78, p <0.001) after adjustment for confounders. In conclusion, echo Doppler EDPR gradient might be a noninvasive predictor of clinical outcomes in hospitalized patients with HF. Copyright © 2016 Elsevier Inc. All rights reserved.

A microfluidic platform for 3-dimensional cell culture and cell-based assays.

PubMed

Kim, Minseok S; Yeon, Ju Hun; Park, Je-Kyun

2007-02-01

This paper reports a novel microfluidic platform introducing peptide hydrogel to make biocompatible microenvironment as well as realizing in situ cell-based assays. Collagen composite, OPLA and Puramatrix scaffolds are compared to select good environment for human hepatocellular carcinoma cells (HepG2) by albumin measurement. The selected biocompatible self-assembling peptide hydrogel, Puramatrix, is hydrodynamically focused in the middle of main channel of a microfluidic device, and at the same time the cells are 3-dimensionally immobilized and encapsulated without any additional surface treatment. HepG2 cells have been 3-dimensionally cultured in a poly(dimethylsiloxane) (PDMS) microfluidic device for 4 days. The cells cultured in micro peptide scaffold are compared with those cultured by conventional petri dish in morphology and the rate of albumin secretion. By injection of different reagents into either side of the peptide scaffold, the microfluidic device also forms a linear concentration gradient profile across the peptide scaffold due to molecular diffusion. Based on this characteristic, toxicity tests are performed by Triton X-100. As the higher toxicant concentration gradient forms, the wider dead zone of cells in the peptide scaffold represents. This microfluidic platform facilitates in vivo-like 3-dimensional microenvironment, and have a potential for the applications of reliable cell-based screening and assays including cytotoxicity test, real-time cell viability monitoring, and continuous dose-response assay.

Formation of the long range Dpp morphogen gradient.

PubMed

Schwank, Gerald; Dalessi, Sascha; Yang, Schu-Fee; Yagi, Ryohei; de Lachapelle, Aitana Morton; Affolter, Markus; Bergmann, Sven; Basler, Konrad

2011-07-01

The TGF-β homolog Decapentaplegic (Dpp) acts as a secreted morphogen in the Drosophila wing disc, and spreads through the target tissue in order to form a long range concentration gradient. Despite extensive studies, the mechanism by which the Dpp gradient is formed remains controversial. Two opposing mechanisms have been proposed: receptor-mediated transcytosis (RMT) and restricted extracellular diffusion (RED). In these scenarios the receptor for Dpp plays different roles. In the RMT model it is essential for endocytosis, re-secretion, and thus transport of Dpp, whereas in the RED model it merely modulates Dpp distribution by binding it at the cell surface for internalization and subsequent degradation. Here we analyzed the effect of receptor mutant clones on the Dpp profile in quantitative mathematical models representing transport by either RMT or RED. We then, using novel genetic tools, experimentally monitored the actual Dpp gradient in wing discs containing receptor gain-of-function and loss-of-function clones. Gain-of-function clones reveal that Dpp binds in vivo strongly to the type I receptor Thick veins, but not to the type II receptor Punt. Importantly, results with the loss-of-function clones then refute the RMT model for Dpp gradient formation, while supporting the RED model in which the majority of Dpp is not bound to Thick veins. Together our results show that receptor-mediated transcytosis cannot account for Dpp gradient formation, and support restricted extracellular diffusion as the main mechanism for Dpp dispersal. The properties of this mechanism, in which only a minority of Dpp is receptor-bound, may facilitate long-range distribution.

Fish as indicators of disturbance in streams used for snorkeling activities in a tourist region.

PubMed

Teresa, Fabricio Barreto; Romero, Renato de Mei; Casatti, Lilian; Sabino, José

2011-05-01

A set of metrics that reflect various aspects of population and fish community structure in streams used for snorkeling was evaluated in the tourist region of Bodoquena Plateau, Brazil, with the purpose of biomonitoring the impacts of such activities. Observations were made while snorkeling in two sites (active = with tourism; inactive = without tourism) and along the gradient of daily tourist activity (before, during and after the passage of tourists) in two streams. Five metrics discriminated active from inactive sites: (i) the abundance of Crenicichla lepidota and (ii) the incidence of reproductive activity in Crenicichla lepidota which were greater in inactive sites, regardless the gradient of daily tourist activity; (iii) the feeding pattern of Prochilodus lineatus, which differed among sites and along the gradient of daily tourist activity; (iv) the abundance of Moenkhausia bonita, which was higher in the active sites and significantly increased along the gradient of daily tourist activity in one stream but decrease along the gradient in other stream; (v) the abundance of Hyphessobrycon eques, which was greater in inactive sites, regardless the gradient of daily tourist activity. With the exception of metric "iv", the metrics were mediated by the reduction in habitat structural complexity due to snorkeling disturbance. The definition of these metrics is relevant because the degradation of ecosystem structural elements is one of the main impacts of recreational activities on aquatic environments. The easy recognition of target species and high water transparency throughout the year ensures the feasibility of these metrics in monitoring programs and may be applied by technicians after quick guides and training.

Fish as Indicators of Disturbance in Streams Used for Snorkeling Activities in a Tourist Region

NASA Astrophysics Data System (ADS)

Teresa, Fabricio Barreto; Romero, Renato De Mei; Casatti, Lilian; Sabino, José

2011-05-01

A set of metrics that reflect various aspects of population and fish community structure in streams used for snorkeling was evaluated in the tourist region of Bodoquena Plateau, Brazil, with the purpose of biomonitoring the impacts of such activities. Observations were made while snorkeling in two sites (active = with tourism; inactive = without tourism) and along the gradient of daily tourist activity (before, during and after the passage of tourists) in two streams. Five metrics discriminated active from inactive sites: (i) the abundance of Crenicichla lepidota and (ii) the incidence of reproductive activity in Crenicichla lepidota which were greater in inactive sites, regardless the gradient of daily tourist activity; (iii) the feeding pattern of Prochilodus lineatus, which differed among sites and along the gradient of daily tourist activity; (iv) the abundance of Moenkhausia bonita, which was higher in the active sites and significantly increased along the gradient of daily tourist activity in one stream but decrease along the gradient in other stream; (v) the abundance of Hyphessobrycon eques, which was greater in inactive sites, regardless the gradient of daily tourist activity. With the exception of metric "iv", the metrics were mediated by the reduction in habitat structural complexity due to snorkeling disturbance. The definition of these metrics is relevant because the degradation of ecosystem structural elements is one of the main impacts of recreational activities on aquatic environments. The easy recognition of target species and high water transparency throughout the year ensures the feasibility of these metrics in monitoring programs and may be applied by technicians after quick guides and training.

Temperature gradient interaction chromatography of polymers: A molecular statistical model.

PubMed

Radke, Wolfgang; Lee, Sekyung; Chang, Taihyun

2010-11-01

A new model describing the retention in temperature gradient interaction chromatography of polymers is developed. The model predicts that polymers might elute in temperature gradient interaction chromatography in either an increasing or decreasing order or even nearly independent of molar mass, depending on the rate of the temperature increase relative to the flow rate. This is in contrast to solvent gradient elution, where polymers elute either in order of increasing molar mass or molar mass independent. The predictions of the newly developed model were verified with the literature data as well as new experimental data. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Patterns of ecology and distribution of the tree crickets Oecanthus dulcisonans and O. pellucens (Orthoptera: Gryllidae; Oecanthinae) in southern Italy.

PubMed

Labadessa, Rocco; Todisco, Simone

2016-09-21

Ecological proclivities of the tree cricket Oecanthus dulcisonans, which has been recently separated from the well known O. pellucens, have been poorly investigated. Moreover, studies on the patterns driving niche segregation between these related species are still needed. This study aims to explore the role of macro-habitat in shaping the distribution of O. dulcisonans and O. pellucens in southern Italy. Occurrence data were sampled by means of a rapid acoustic survey scheme at a regional scale. The significance of difference in ecological and habitat parameters was tested between the two species. While confirming species behavioural patterns, new findings are provided with regard to their ecological difference. Species distribution proves to be primarily driven by elevation gradient and temperature variation, with O. dulcisonans common and widespread in warmer lowlands and O. pellucens limited to higher altitudes. The two species also show significant spatial segregation within vegetation layers. These findings also provide cues for future studies on species interaction, as well as for the assessment of a monitoring scheme which may help interpreting the effects of broad environmental changes.

Producibility improvements suggested by a validated process model of seeded CdZnTe vertical Bridgman growth

NASA Astrophysics Data System (ADS)

Larson, David J., Jr.; Casagrande, Louis G.; Di Marzio, Don; Levy, Alan; Carlson, Frederick M.; Lee, Taipao; Black, David R.; Wu, Jun; Dudley, Michael

1994-07-01

We have successfully validated theoretical models of seeded vertical Bridgman-Stockbarger CdZnTe crystal growth and post-solidification processing, using in-situ thermal monitoring and innovative material characterization techniques. The models predict the thermal gradients, interface shape, fluid flow and solute redistribution during solidification, as well as the distributions of accumulated excess stress that causes defect generation and redistribution. Data from the furnace and ampoule wall have validated predictions from the thermal model. Results are compared to predictions of the thermal and thermo-solutal models. We explain the measured initial, change-of-rate, and terminal compositional transients as well as the macrosegregation. Macro and micro-defect distributions have been imaged on CdZnTe wafers from 40 mm diameter boules. Superposition of topographic defect images and predicted excess stress patterns suggests the origin of some frequently encountered defects, particularly on a macro scale, to result from the applied and accumulated stress fields and the anisotropic nature of the CdZnTe crystal. Implications of these findings with respect to producibility are discussed.

Thermal management of an unconsolidated shallow urban groundwater body

NASA Astrophysics Data System (ADS)

Epting, J.; Händel, F.; Huggenberger, P.

2013-05-01

This study presents the development of tools for the sustainable thermal management of a shallow unconsolidated urban groundwater body in the city of Basel (Switzerland). The concept of the investigations is based on (1) a characterization of the present thermal state of the urban groundwater body, and (2) the evaluation of potential mitigation measures for the future thermal management of specific regions within the groundwater body. The investigations focus on thermal processes down-gradient of thermal groundwater use, effects of heated buildings in the subsurface as well as the thermal influence of river-groundwater interaction. Investigation methods include (1) short- and long-term data analysis, (2) high-resolution multilevel groundwater temperature monitoring, as well as (3) 3-D numerical groundwater flow and heat transport modeling and scenario development. The combination of these methods allows for the quantifying of the thermal influences on the investigated urban groundwater body, including the influences of thermal groundwater use and heated subsurface constructions. Subsequently, first implications for management strategies are discussed, including minimizing further groundwater temperature increase, targeting "potential natural" groundwater temperatures for specific aquifer regions and exploiting the thermal potential.

Metallic and Ceramic Thin Film Thermocouples for Gas Turbine Engines

PubMed Central

Tougas, Ian M.; Amani, Matin; Gregory, Otto J.

2013-01-01

Temperatures of hot section components in today's gas turbine engines reach as high as 1,500 °C, making in situ monitoring of the severe temperature gradients within the engine rather difficult. Therefore, there is a need to develop instrumentation (i.e., thermocouples and strain gauges) for these turbine engines that can survive these harsh environments. Refractory metal and ceramic thin film thermocouples are well suited for this task since they have excellent chemical and electrical stability at high temperatures in oxidizing atmospheres, they are compatible with thermal barrier coatings commonly employed in today's engines, they have greater sensitivity than conventional wire thermocouples, and they are non-invasive to combustion aerodynamics in the engine. Thin film thermocouples based on platinum:palladium and indium oxynitride:indium tin oxynitride as well as their oxide counterparts have been developed for this purpose and have proven to be more stable than conventional type-S and type-K thin film thermocouples. The metallic and ceramic thin film thermocouples described within this paper exhibited remarkable stability and drift rates similar to bulk (wire) thermocouples. PMID:24217356

PWV, Temperature and Wind Statistics at Sites Suitable For mm and Sub-mm Wavelengths Astronomy

NASA Astrophysics Data System (ADS)

Otarola, Angel; Travouillon, Tony; De Breuck, Carlos; Radford, Simon; Matsushita, Satoki; Pérez-Beaupuits, Juan P.

2018-01-01

Atmospheric water vapor is the main limiting factor of atmospheric transparency in the mm and sub-mm wavelength spectral windows. Thus, dry sites are needed for the installation and successful operation of radio astronomy observatories exploiting those spectral windows. Other parameters that play an important role in the mechanical response of radio telescopes exposed to the environmental conditions are: temperature, and in particular temperature gradients that induce thermal deformation of mechanical structures, as well as wind magnitude that induce pointing jitter affecting this way the required accuracy in the ability to point to a cosmic source during the observations. Temperature and wind are variables of special consideration when planning the installation and operations of large aperture radio telescopes. This work summarizes the statistics of precipitable water vapor (PWV), temperature and wind monitored at sites by the costal mountain range, as well as on t he west slope of the Andes mountain range in the region of Antofagasta, Chile. This information could prove useful for the planning of the Atacama Large-Aperture Submm/mm Telescope (AtLast).

Metallic and ceramic thin film thermocouples for gas turbine engines.

PubMed

Tougas, Ian M; Amani, Matin; Gregory, Otto J

2013-11-08

Temperatures of hot section components in today's gas turbine engines reach as high as 1,500 °C, making in situ monitoring of the severe temperature gradients within the engine rather difficult. Therefore, there is a need to develop instrumentation (i.e., thermocouples and strain gauges) for these turbine engines that can survive these harsh environments. Refractory metal and ceramic thin film thermocouples are well suited for this task since they have excellent chemical and electrical stability at high temperatures in oxidizing atmospheres, they are compatible with thermal barrier coatings commonly employed in today's engines, they have greater sensitivity than conventional wire thermocouples, and they are non-invasive to combustion aerodynamics in the engine. Thin film thermocouples based on platinum:palladium and indium oxynitride:indium tin oxynitride as well as their oxide counterparts have been developed for this purpose and have proven to be more stable than conventional type-S and type-K thin film thermocouples. The metallic and ceramic thin film thermocouples described within this paper exhibited remarkable stability and drift rates similar to bulk (wire) thermocouples.

High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol.

PubMed

Andeer, Peter; Strand, Stuart E; Stahl, David A

2012-01-01

Stable-isotope probing (SIP) has proved a valuable cultivation-independent tool for linking specific microbial populations to selected functions in various natural and engineered systems. However, application of SIP to microbial populations with relatively minor buoyant density increases, such as populations that utilize compounds as a nitrogen source, results in reduced resolution of labeled populations. We therefore developed a tandem quantitative PCR (qPCR)-TRFLP (terminal restriction fragment length polymorphism) protocol that improves resolution of detection by quantifying specific taxonomic groups in gradient fractions. This method combines well-controlled amplification with TRFLP analysis to quantify relative taxon abundance in amplicon pools of FAM-labeled PCR products, using the intercalating dye EvaGreen to monitor amplification. Method accuracy was evaluated using mixtures of cloned 16S rRNA genes, DNA extracted from low- and high-G+C bacterial isolates (Escherichia coli, Rhodococcus, Variovorax, and Microbacterium), and DNA from soil microcosms amended with known amounts of genomic DNA from bacterial isolates. Improved resolution of minor shifts in buoyant density relative to TRFLP analysis alone was confirmed using well-controlled SIP analyses.

Estimation of the depth to the fresh-water/salt-water interface from vertical head gradients in wells in coastal and island aquifers

NASA Astrophysics Data System (ADS)

Izuka, Scot K.; Gingerich, Stephen B.

An accurate estimate of the depth to the theoretical interface between fresh, water and salt water is critical to estimates of well yields in coastal and island aquifers. The Ghyben-Herzberg relation, which is commonly used to estimate interface depth, can greatly underestimate or overestimate the fresh-water thickness, because it assumes no vertical head gradients and no vertical flow. Estimation of the interface depth needs to consider the vertical head gradients and aquifer anisotropy that may be present. This paper presents a method to calculate vertical head gradients using water-level measurements made during drilling of a partially penetrating well; the gradient is then used to estimate interface depth. Application of the method to a numerically simulated fresh-water/salt-water system shows that the method is most accurate when the gradient is measured in a deeply penetrating well. Even using a shallow well, the method more accurately estimates the interface position than does the Ghyben-Herzberg relation where substantial vertical head gradients exist. Application of the method to field data shows that drilling, collection methods of water-level data, and aquifer inhomogeneities can cause difficulties, but the effects of these difficulties can be minimized. Résumé Une estimation précise de la profondeur de l'interface théorique entre l'eau douce et l'eau salée est un élément critique dans les estimations de rendement des puits dans les aquifères insulaires et littoraux. La relation de Ghyben-Herzberg, qui est habituellement utilisée pour estimer la profondeur de cette interface, peut fortement sous-estimer ou surestimer l'épaisseur de l'eau douce, parce qu'elle suppose l'absence de gradient vertical de charge et d'écoulement vertical. L'estimation de la profondeur de l'interface requiert de prendre en considération les gradients verticaux de charge et l'éventuelle anisotropie de l'aquifère. Cet article propose une méthode de calcul des gradients verticaux de charge à partir des mesures de niveau piézométrique faites en cours de foration d'un puits incomplet; le gradient est alors utilisé pour estimer la profondeur de l'interface. L'application de cette méthode à un système eau douce - eau salée simulé numériquement montre que la méthode est la plus précise lorsque le gradient est mesuré dans un puits pénétrant profondément dans l'aquifère. Même en utilisant un puits peu profond, la méthode estime la position de l'interface avec plus de précision que ne le fait la relation de Ghyben-Herzberg lorsqu'il existe un gradient vertical de charge bien marqué. L'application de la méthode à des données de terrain montre que la foration, les méthodes de mesure de niveau et les hétérogénéités au sein de l'aquifère peuvent être la cause de difficultés, mais que les effets de ces difficultés peuvent être réduits. Resumen Para la estimación de la productividad de pozos en acuíferos costeros y en islas es necesaria una estimación precisa de la profundidad de la interfaz teórica entre agua dulce y agua salada. La relación de Ghyben-Herzberg, usada habitualmente para estimar la profundidad de la interfaz, puede subestimar o sobrestimar el espesor de agua dulce, al asumir la ausencia de flujos y gradientes verticales. La estimación de la profundidad de la interfaz debe considerar tanto estos gradientes verticales, como la posible anisotropía del acuífero. En este artículo se presenta un método para calcular los gradientes verticales de niveles a partir de las medidas obtenidas durante la perforación de un pozo parcialmente penetrante para, a partir de este gradiente, estimar la profundidad de la interfaz. La aplicación del método a un sistema de agua dulce/agua salada simulado numéricamente muestra que el método es más preciso cuando el gradiente se mide en un pozo profundo. Incluso en el caso de un pozo superficial, el método permite una estimación más precisa de la profundidad de la interfaz que la aplicación de la fórmula de Ghyben-Herzberg, en los casos en los que existen gradientes verticales significativos. La aplicación del método a datos reales muestra que la perforación, la recogida de datos de niveles y la heterogeneidad en el acuífero pueden causar dificultades en la aplicación del método, pero que estas pueden minimizarse.

Identifying Stream/Aquifer Exchange by Temperature Gradient in a Guarani Aquifer System Outcrop Zone

NASA Astrophysics Data System (ADS)

Wendland, E.; Rosa, D. M. S.; Anache, J. A. A.; Lowry, C.; Lin, Y. F. F.

2017-12-01

Recharge of the Guarani Aquifer System (GAS) in South America is supposed to occur mainly in the outcrop zones, where the GAS appears as an unconfined aquifer (10% of the 1.2 Million km2 aquifer extension). Previous evaluations of recharge are based essentially on water balance estimates for the whole aquifer area or water table fluctuations in monitoring wells. To gain a more detailed understanding of the recharge mechanisms the present work aimed to study the stream aquifer interaction in a watershed (Ribeirão da Onça) at an outcrop zone. Two Parshall flumes were installed 1.3 km apart for discharge measurement in the stream. Along this distance an optic fiber cable was deployed to identify stretches with gaining and losing behavior. In order to estimate groundwater discharge in specific locations, 8 temperature sticks were set up along the stream reach to measure continuously the vertical temperature gradient. A temperature probe with 4 thermistors was also used to map the shallow streambed temperature gradient manually along the whole distance. The obtained results show a discharge difference of 250 m3/h between both flumes. Since the last significant rainfall (15 mm) in the watershed occurred 3 months ago, this value can be interpreted as the base flow contribution to the stream during the dry season. Given the temperature difference between groundwater ( 24oC) and surface water ( 17oC) the fiber-optic distributed temperature sensing (FO-DTS) allowed the identification of stretches with gaining behavior. Temperature gradients observed at the streambed varied between 0.67 and 14.33 oC/m. The study demonstrated that heat may be used as natural tracer even in tropical conditions, where the groundwater temperature is higher than the surface water temperature during the winter. The obtained results show that the discharge difference between both flumes can not be extrapolated without detailed analysis. Gaining and loosing stretches have to be identified on order to estimate total base flow contribution of the watershed. This result is important to correct the water balance of the system.

Validation of a multilevel sampling device to determine the vertical variability of chlorinated solvent in a contaminated aquifer.

PubMed

Barnier, C; Palmier, C; Atteia, O

2013-01-01

The vertical heterogeneity of contaminant concentrations in aquifers is well known, but obtaining representative samples is still a subject of debate. In this paper, the question arises from sites where numerous fully screened wells exist and there is a need to define the vertical distribution of contaminants. For this purpose, several wells were investigated with different techniques on a site contaminated with chlorinated solvents. A core-bored well shows that a tetrachloroethene (PCE) phase is sitting on and infiltrating a less permeable layer. Downstream of the cored well, the following sampling techniques were compared on fully screened wells: low flow pumping at several depths, pumping between packers and a new multilevel sampler for fully screened wells. Concerning low flow rate pumping, very low gradients were found, which may be due to the existence of vertical flow inside the well or in the gravel pack. Sampling between packers gave results comparable with the cores, separating a layer with PCE and trichloroethene from another one with cis 1,2-dichloroethene and vinyl chloride as major compounds. Detailed sampling according to pumped volume shows that even between packers, cleaning of the inter-packer volume is necessary before each sampling. Lastly, the proposed new multilevel sampler gives results similar to the packers but has the advantages of much faster sampling and a constant vertical positioning, which is fairly important for long-term monitoring in highly stratified aquifers.

Wavefront image sensor chip

PubMed Central

Cui, Xiquan; Ren, Jian; Tearney, Guillermo J.; Yang, Changhuei

2010-01-01

We report the implementation of an image sensor chip, termed wavefront image sensor chip (WIS), that can measure both intensity/amplitude and phase front variations of a light wave separately and quantitatively. By monitoring the tightly confined transmitted light spots through a circular aperture grid in a high Fresnel number regime, we can measure both intensity and phase front variations with a high sampling density (11 µm) and high sensitivity (the sensitivity of normalized phase gradient measurement is 0.1 mrad under the typical working condition). By using WIS in a standard microscope, we can collect both bright-field (transmitted light intensity) and normalized phase gradient images. Our experiments further demonstrate that the normalized phase gradient images of polystyrene microspheres, unstained and stained starfish embryos, and strongly birefringent potato starch granules are improved versions of their corresponding differential interference contrast (DIC) microscope images in that they are artifact-free and quantitative. Besides phase microscopy, WIS can benefit machine recognition, object ranging, and texture assessment for a variety of applications. PMID:20721059

Intense deformation field at oceanic front inferred from directional sea surface roughness observations

NASA Astrophysics Data System (ADS)

Rascle, Nicolas; Molemaker, Jeroen; Marié, Louis; Nouguier, Frédéric; Chapron, Bertrand; Lund, Björn; Mouche, Alexis

2017-06-01

Fine-scale current gradients at the ocean surface can be observed by sea surface roughness. More specifically, directional surface roughness anomalies are related to the different horizontal current gradient components. This paper reports results from a dedicated experiment during the Lagrangian Submesoscale Experiment (LASER) drifter deployment. A very sharp front, 50 m wide, is detected simultaneously in drifter trajectories, sea surface temperature, and sea surface roughness. A new observational method is applied, using Sun glitter reflections during multiple airplane passes to reconstruct the multiangle roughness anomaly. This multiangle anomaly is consistent with wave-current interactions over a front, including both cross-front convergence and along-front shear with cyclonic vorticity. Qualitatively, results agree with drifters and X-band radar observations. Quantitatively, the sharpness of roughness anomaly suggests intense current gradients, 0.3 m s-1 over the 50 m wide front. This work opens new perspectives for monitoring intense oceanic fronts using drones or satellite constellations.

Method and system for optical figuring by imagewise heating of a solvent

DOEpatents

Rushford, Michael C.

2005-08-30

A method and system of imagewise etching the surface of a substrate, such as thin glass, in a parallel process. The substrate surface is placed in contact with an etchant solution which increases in etch rate with temperature. A local thermal gradient is then generated in each of a plurality of selected local regions of a boundary layer of the etchant solution to imagewise etch the substrate surface in a parallel process. In one embodiment, the local thermal gradient is a local heating gradient produced at selected addresses chosen from an indexed array of addresses. The activation of each of the selected addresses is independently controlled by a computer processor so as to imagewise etch the substrate surface at region-specific etch rates. Moreover, etching progress is preferably concurrently monitored in real time over the entire surface area by an interferometer so as to deterministically control the computer processor to image-wise figure the substrate surface where needed.

In Vivo Evidence for a Lactate Gradient from Astrocytes to Neurons.

PubMed

Mächler, Philipp; Wyss, Matthias T; Elsayed, Maha; Stobart, Jillian; Gutierrez, Robin; von Faber-Castell, Alexandra; Kaelin, Vincens; Zuend, Marc; San Martín, Alejandro; Romero-Gómez, Ignacio; Baeza-Lehnert, Felipe; Lengacher, Sylvain; Schneider, Bernard L; Aebischer, Patrick; Magistretti, Pierre J; Barros, L Felipe; Weber, Bruno

2016-01-12

Investigating lactate dynamics in brain tissue is challenging, partly because in vivo data at cellular resolution are not available. We monitored lactate in cortical astrocytes and neurons of mice using the genetically encoded FRET sensor Laconic in combination with two-photon microscopy. An intravenous lactate injection rapidly increased the Laconic signal in both astrocytes and neurons, demonstrating high lactate permeability across tissue. The signal increase was significantly smaller in astrocytes, pointing to higher basal lactate levels in these cells, confirmed by a one-point calibration protocol. Trans-acceleration of the monocarboxylate transporter with pyruvate was able to reduce intracellular lactate in astrocytes but not in neurons. Collectively, these data provide in vivo evidence for a lactate gradient from astrocytes to neurons. This gradient is a prerequisite for a carrier-mediated lactate flux from astrocytes to neurons and thus supports the astrocyte-neuron lactate shuttle model, in which astrocyte-derived lactate acts as an energy substrate for neurons. Copyright © 2016 Elsevier Inc. All rights reserved.

Assessing coral reef health across onshore to offshore stress gradients in the US Virgin Islands.

PubMed

Smith, T B; Nemeth, R S; Blondeau, J; Calnan, J M; Kadison, E; Herzlieb, S

2008-12-01

Managing the effects of anthropogenic disturbance on coral reefs is highly dependant on effective strategies to assess degradation and recovery. We used five years of field data in the US Virgin Islands to investigate coral reef response to a potential gradient of stress. We found that the prevalence of old partial mortality, bleaching, and all forms of coral health impairment (a novel category) increased with nearshore anthropogenic processes, such as a five-fold higher rate of clay and silt sedimentation. Other patterns of coral health, such as recent partial mortality, other diseases, and benthic cover, did not respond to this potential gradient of stress or their response could not be resolved at the frequency or scale of monitoring. We suggest that persistent signs of disturbance are more useful to short-term, non-intensive (annual) coral reef assessments, but more intensive (semi-annual) assessments are necessary to resolve patterns of transient signs of coral health impairment.

SDSS-IV MaNGA: The Spatially Resolved Stellar Initial Mass Function in ˜400 Early-Type Galaxies

NASA Astrophysics Data System (ADS)

Parikh, Taniya; Thomas, Daniel; Maraston, Claudia; Westfall, Kyle B.; Goddard, Daniel; Lian, Jianhui; Meneses-Goytia, Sofia; Jones, Amy; Vaughan, Sam; Andrews, Brett H.; Bershady, Matthew; Bizyaev, Dmitry; Brinkmann, Jonathan; Brownstein, Joel R.; Bundy, Kevin; Drory, Niv; Emsellem, Eric; Law, David R.; Newman, Jeffrey A.; Roman-Lopes, Alexandre; Wake, David; Yan, Renbin; Zheng, Zheng

2018-03-01

MaNGA provides the opportunity to make precise spatially resolved measurements of the IMF slope in galaxies owing to its unique combination of spatial resolution, wavelength coverage and sample size. We derive radial gradients in age, element abundances and IMF slope analysing optical and near-infrared absorption features from stacked spectra out to the half-light radius of 366 early-type galaxies with masses 9.9 - 10.8 log M/M⊙. We find flat gradients in age and [α/Fe] ratio, as well as negative gradients in metallicity, consistent with the literature. We further derive significant negative gradients in the [Na/Fe] ratio with galaxy centres being well enhanced in Na abundance by up to 0.5 dex. Finally, we find a gradient in IMF slope with a bottom-heavy IMF in the centre (typical mass excess factor of 1.5) and a Milky Way-type IMF at the half-light radius. This pattern is mass-dependent with the lowest mass galaxies in our sample featuring only a shallow gradient around a Milky Way IMF. Our results imply the local IMF-σ relation within galaxies to be even steeper than the global relation and hint towards the local metallicity being the dominating factor behind the IMF variations. We also employ different stellar population models in our analysis and show that a radial IMF gradient is found independently of the stellar population model used. A similar analysis of the Wing-Ford band provides inconsistent results and further evidence of the difficulty in measuring and modelling this particular feature.

Sub-arcsecond, differential deflectometry to measure thermally induced distortions of the Swift optical bench

NASA Astrophysics Data System (ADS)

Leviton, Douglas B.; Frey, Brad J.; Madison, Larry E.; Parker, James A.; Sheinman, Oren E.

2003-03-01

The Swift optical bench is a roughly 2.7 m diameter, 0.1 m thick composite structure carrying the Burst Alert Telescope (BAT), X-ray Telescope (XRT), and the Ultraviolet Optical Telescope (UVOT) as well as various attitude control instrumentation for the spacecraft. A high precision test of the optical bench using multi-aperture optical deflectometry was developed to verify that the relative boresights of the XRT and UVOT instruments would not change by more than several arcseconds when a worst case on-orbit temperature gradient is imposed through the thickness of the bench. Results of validation tests in a laminar flow cleanroom environment without vibration isolation demonstrated a differential measurement capability with 0.2 arcsecond sensitivity and 0.5 arcsecond accuracy per day. The technique is easily adaptable to similar deflection monitoring requirements for other large spacecraft structures.

Transsphenoidal pituitary resection with intraoperative MR guidance: preliminary results

NASA Astrophysics Data System (ADS)

Pergolizzi, Richard S., Jr.; Schwartz, Richard B.; Hsu, Liangge; Wong, Terence Z.; Black, Peter M.; Martin, Claudia; Jolesz, Ferenc A.

1999-05-01

The use of intraoperative MR image guidance has the potential to improve the precision, extent and safety of transsphenoidal pituitary resections. At Brigham and Women's Hospital, an open-bore configuration 0.5T MR system (SIGNA SP, GE Medical Systems, Milwaukee, WI) has been used to provide image guidance for nine transsphenoidal pituitary adenoma resections. The intraoperative MR system allowed the radiologist to direct the surgeon toward the sella turcica successfully while avoiding the cavernous sinus, optic chiasm and other sensitive structures. Imaging performed during the surgery monitored the extent of resection and allowed for removal of tumor beyond the surgeon's view in five cases. Dynamic MR imaging was used to distinguish residual tumor from normal gland and postoperative changes permitting more precise tumor localization. A heme-sensitive long TE gradient echo sequence was used to evaluate for the presence of hemorrhagic debris. All patients tolerated the procedure well without significant complications.

Clinical, analytical and bioimpedance characteristics of persistently overhydrated haemodialysis patients.

PubMed

Castellano, Sandra; Palomares, Inés; Molina, Manuel; Pérez-García, Rafael; Aljama, Pedro; Ramos, Rosa; Merello, J Ignacio

2014-11-17

Fluid overload is an important and modifiable cardiovascular risk factor for haemodialysis patients. So far, the diagnosis was based on clinical methods alone. Nowadays, we have new tools to assess more objectively the hydration status of the patients on haemodialysis, as BCM (Body Composition Monitor). A Relative Overhydration (AvROH) higher than 15% (it means, Absolute Overhydration or AWOH higher than 2.5 Litres) is associated to greater risk in haemodialysis. However, there is a group of maintained hyperhydrated patients. The aim of the present study is to identify the characteristics of patients with maintained hyperhydrated status (AvROH higher than 15% or AWOH higher than 2.5 liters). The secondary aim is to show the hemodynamic and analytical changes that are related to the reduction in hyperhydration status. Longitudinal cohort study during six months in 2959 patients in haemodialysis (HD) that are grouped according to their hydration status by BCM. And we compare their clinical, analytical and bioimpedance spectroscopy parameters. The change in overhydration status is followed by a decrease in blood pressure and the need for hypotensive drugs (AHT) and erythropoiesis stimulating agents (ESA). The target hydration status is not reached by two subgroups of patients. First, in diabetic patients with a high comorbidity index and high number of hypotensive drugs (AHT) but a great positive sodium gradient during dialysis sessions; and, younger non-diabetic patients with longer time on hemodialysis and positive sodium gradient, lower fat tissue index (FTI) but similar lean tissue index (LTI) and albumin than those with a reduction in hyperhydration status. Those patients with a reduction in hyperhydration status, also show a better control in blood pressure and anemia with less number of AHT and ESA. The maintained hyperhydrated patients, diabetic patients with many comorbidities and young men patients with longer time on hemodialysis and non-adherence treatment, can profit from a constant monitoring of their hydration state as well as an individualized treatment (dialysis and drugs).

Development of improved wildfire smoke exposure estimates for health studies in the western U.S.

NASA Astrophysics Data System (ADS)

Ivey, C.; Holmes, H.; Loria Salazar, S. M.; Pierce, A.; Liu, C.

2016-12-01

Wildfire smoke exposure is a significant health concern in the western U.S. because large wildfires have increased in size and frequency over the past four years due to drought conditions. The transport phenomena in complex terrain and timing of the wildfire emissions make the smoke plumes difficult to simulate using conventional air quality models. Monitoring data can be used to estimate exposure metrics, but in rural areas the monitoring networks are too sparse to calculate wildfire exposure metrics for the entire population in a region. Satellite retrievals provide global, spatiotemporal air quality information and are used to track pollution plumes, estimate human exposures, model emissions, and determine sources (i.e., natural versus anthropogenic) in regulatory applications. Particulate matter (PM) exposures can be estimated using columnar aerosol optical depth (AOD), where satellite AOD retrievals serve as a spatial surrogate to simulate surface PM gradients. These exposure models have been successfully used in health effects studies in the eastern U.S. where complex mountainous terrain and surface reflectance do not limit AOD retrival from satellites. Using results from a chemical transport model (CTM) is another effective method to determine spatial gradients of pollutants. However, the CTM does not adequately capture the temporal and spatial distribution of wildfire smoke plumes. By combining the spatiotemporal pollutant fields from both satellite retrievals and CTM results with ground based pollutant observations the spatial wildfire smoke exposure model can be improved. This work will address the challenge of understanding the spatiotemporal distributions of pollutant concentrations to model human exposures of wildfire smoke in regions with complex terrain, where meteorological conditions as well as emission sources significantly influence the spatial distribution of pollutants. The focus will be on developing models to enhance exposure estimates of elevated PM and ozone concentrations from wildfire smoke plumes in the western U.S.

Effects of surface applications of biosolids on soil, crops, ground water, and streambed sediment near Deer Trail, Colorado, 1999-2003

USGS Publications Warehouse

Yager, Tracy J.B.; Smith, David B.; Crock, James G.

2004-01-01

The U.S. Geological Survey, in cooperation with Metro Wastewater Reclamation District and North Kiowa Bijou Groundwater Management District, studied natural geochemical effects and the effects of biosolids applications to the Metro Wastewater Reclamation District properties near Deer Trail, Colorado, during 1999 through 2003 because of public concern about potential contamination of soil, crops, ground water, and surface water from biosolids applications. Parameters analyzed for each monitoring component included arsenic, cadmium, copper, lead, mercury, molybdenum, nickel, selenium, and zinc (the nine trace elements regulated by Colorado for biosolids), gross alpha and gross beta radioactivity, and plutonium, as well as other parameters. Concentrations of the nine regulated trace elements in biosolids were relatively uniform and did not exceed applicable regulatory standards. All plutonium concentrations in biosolids were below the minimum detectable level and were near zero. The most soluble elements in biosolids were arsenic, molybdenum, nickel, phosphorus, and selenium. Elevated concentrations of bismuth, mercury, phosphorus, and silver would be the most likely inorganic biosolids signature to indicate that soil or streambed sediment has been affected by biosolids. Molybdenum and tungsten, and to a lesser degree antimony, cadmium, cobalt, copper, mercury, nickel, phosphorus, and selenium, would be the most likely inorganic 'biosolids signature' to indicate ground water or surface water has been affected by biosolids. Soil data indicate that biosolids have had no measurable effect on the concentration of the constituents monitored. Arsenic concentrations in soil of both Arapahoe and Elbert County monitoring sites (like soil from all parts of Colorado) exceed the Colorado soil remediation objectives and soil cleanup standards, which were determined by back-calculating a soil concentration equivalent to a one-in-a-million cumulative cancer risk. Lead concentrations in soil slightly exceed the U.S. Environmental Protection Agency toxicity-derived ecological soil-screening levels for avian wildlife. Plutonium concentration in the soil was near zero. Wheat-grain data were insufficient to determine any measurable effects from biosolids. Comparison with similar data from other parts of North America where biosolids were not applied indicates similar concentrations. However, the Deer Trail study area had higher nickel concentrations in wheat from both the biosolids-applied fields and the control fields. Plutonium content of the wheat was near zero. Ground-water levels generally declined at most wells during 1999 through 2003. Ground-water quality did not correlate with ground-water levels. Vertical ground-water gradients during 1999 through 2003 indicate that bedrock ground-water resources downgradient from the biosolids-applied areas are not likely to be contaminated by biosolids applications unless the gradients change as a result of pumping. Ground-water quality throughout the study area varied over time at each site and from site to site at the same time, but plutonium concentrations in the ground water always were near zero. Inorganic concentrations at well D6 were relatively high compared to other ground-water sites studied. Ground-water pH and concentrations of fluoride, nitrite, aluminum, arsenic, barium, chromium, cobalt, copper, lead, mercury, nickel, silver, zinc, and plutonium in the ground water of the study area met Colorado standards. Concentrations of chloride, sulfate, nitrate, boron, iron, manganese, and selenium exceeded Colorado ground-water standards at one or more wells. Nitrate concentrations at well D6 significantly (alpha = 0.05) exceeded the Colorado regulatory standard. Concentrations of arsenic, cadmium, chromium, lead, mercury, nickel, and zinc in ground water had no significant (alpha = 0.05) upward trends. During 1999-2003, concentrations of nitrate, copper, molybdenum, and selenium

An overview of wave-mean flow interactions during the winter of 1978-79 derived from LIMS observations. [Limb Infrared Monitor of Stratosphere

NASA Technical Reports Server (NTRS)

Gille, J. C.; Lyjak, L. V.

1984-01-01

Gradient winds, Eliassen-Palm (EP) fluxes and flux divergences, and the squared refractive index for planetary waves have been calculated from mapped data from the Limb Infrared Monitor of the Stratosphere (LIMS) experiment on Nimbus 7. The changes in the zonal mean atmospheric state, from early winter through 3 disturbances, is described. Convergence or divergence of the EP fluxes clearly produces changes in the zonal mean wind. The steering of the waves by the refractive index structure is not as clear on a daily basis.

A low-cost particulate matter (PM2.5) monitor for wildland fire smoke

NASA Astrophysics Data System (ADS)

Kelleher, Scott; Quinn, Casey; Miller-Lionberg, Daniel; Volckens, John

2018-02-01

Wildfires and prescribed fires produce emissions that degrade visibility and are harmful to human health. Smoke emissions and exposure monitoring is critical for public and environmental health protection; however, ground-level measurements of smoke from wildfires and prescribed fires has proven difficult, as existing (validated) monitoring technologies are expensive, cumbersome, and generally require line power. Few ground-based measurements are made during fire events, which limits our ability to assess the environmental and human health impacts of wildland fire smoke. The objective of this work was to develop and validate an Outdoor Aerosol Sampler (OAS) - a filter-based air sampler that has been miniaturized, solar powered, and weatherproofed. This sampler was designed to overcome several of the technical challenges of wildland fire monitoring by being relatively inexpensive and solar powered. The sampler design objectives were achieved by leveraging low-cost electronic components, open-source programming platforms, and in-house fabrication methods. A direct-reading PM2.5 sensor was selected and integrated with the OAS to provide time-resolved concentration data. Cellular communications established via short message service (SMS) technology were utilized in transmitting online sensor readings and controlling the sampling device remotely. A Monte Carlo simulation aided in the selection of battery and solar power necessary to independently power the OAS, while keeping cost and size to a minimum. Thirteen OAS were deployed to monitor smoke concentrations downwind from a large prescribed fire. Aerosol mass concentrations were interpolated across the monitoring network to depict smoke concentration gradients in the vicinity of the fire. Strong concentration gradients were observed (spatially and temporally) and likely present due to a combination of changing fire location and intensity, topographical features (e.g., mountain ridges), and diurnal weather patterns. Gravimetric filter measurements made by the OAS (when corrected for filter collection efficiency) showed relatively good agreement with measurements from an EPA federal equivalent monitor. However, the real-time optical sensor (Sharp GP2Y1023AU0F, Sharp Electronic Co.) within the OAS suffered from temperature dependence, drift, and imprecision.

Temperature can interact with landscape factors to affect songbird productivity

Treesearch

W. Andrew Cox; Frank R. III Thompson; Jennifer L. Reidy; John Faaborg

2013-01-01

Increased temperatures and more extreme weather patterns associated with global climate change can interact with other factors that regulate animal populations, but many climate change studies do not incorporate other threats to wildlife in their analyses. We used 20 years of nest-monitoring data from study sites across a gradient of habitat fragmentation in Missouri,...

Indicators of nitrogen status in California forests

Treesearch

Mark E. Fenn; Mark A. Poth

1998-01-01

Indicators of ecosystem nitrogen (N) status are needed for monitoring and for identifying ecosystems that are at risk of becoming N saturated. The N chemistry of a number of plant, soil and hydrologic components were analyzed to assess the N status of mixed conifer forests across an N deposition gradient in the San Bernardino Mountains east of Los Angeles, California....

Determination of the origin of elevated uranium at a Former Air Force Landfill using non-parametric statistics analysis and uranium isotope ratio analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weismann, J.; Young, C.; Masciulli, S.

2007-07-01

Lowry Air Force Base (Lowry) was closed in September 1994 as part of the Base Realignment and Closure (BRAC) program and the base was transferred to the Lowry Redevelopment Authority in 1995. As part of the due diligence activities conducted by the Air Force, a series of remedial investigations were conducted across the base. A closed waste landfill, designated Operable Unit 2 (OU 2), was initially assessed in a 1990 Remedial Investigation (RI; [1]). A Supplemental Remedial Investigation was conducted in 1995 [2] and additional studies were conducted in a 1998 Focused Feasibility Study. [3] The three studies indicated thatmore » gross alpha, gross beta, and uranium concentrations were consistently above regulatory standards and that there were detections of low concentrations other radionuclides. Results from previous investigations at OU 2 have shown elevated gross alpha, gross beta, and uranium concentrations in groundwater, surface water, and sediments. The US Air Force has sought to understand the provenance of these radionuclides in order to determine if they could be due to leachates from buried radioactive materials within the landfill or whether they are naturally-occurring. The Air Force and regulators agreed to use a one-year monitoring and sampling program to seek to explain the origins of the radionuclides. Over the course of the one-year program, dissolved uranium levels greater than the 30 {mu}g/L Maximum Contaminant Level (MCL) were consistently found in both up-gradient and down-gradient wells at OU 2. Elevated Gross Alpha and Gross Beta measurements that were observed during prior investigations and confirmed during the LTM were found to correlate with high dissolved uranium content in groundwater. If Gross Alpha values are corrected to exclude uranium and radon contributions in accordance with US EPA guidance, then the 15 pCi/L gross alpha level is not exceeded. The large dataset also allowed development of gross alpha to total uranium correlation factors so that gross alpha action levels can be applied to future long-term landfill monitoring to track radiological conditions at lower cost. Ratios of isotopic uranium results were calculated to test whether the elevated uranium displayed signatures indicative of military use. Results of all ratio testing strongly supports the conclusion that the uranium found in groundwater, surface water, and sediment at OU 2 is naturally-occurring and has not undergone anthropogenic enrichment or processing. U-234:U-238 ratios also show that a disequilibrium state, i.e., ratio greater than 1, exists throughout OU 2 which is indicative of long-term aqueous transport in aged aquifers. These results all support the conclusion that the elevated uranium observed at OU 2 is due to the high concentrations in the regional watershed. Based on the results of this monitoring program, we concluded that the elevated uranium concentrations measured in OU 2 groundwater, surface water, and sediment are due to the naturally-occurring uranium content of the regional watershed and are not the result of waste burials in the former landfill. Several lines of evidence indicate that natural uranium has been naturally concentrated beneath OU 2 in the geologic past and the higher of uranium concentrations in down-gradient wells is the result of geochemical processes and not the result of a uranium ore disposal. These results therefore provide the data necessary to support radiological closure of OU 2. (authors)« less

State of inequality in malaria intervention coverage in sub-Saharan African countries.

PubMed

Galactionova, Katya; Smith, Thomas A; de Savigny, Don; Penny, Melissa A

2017-10-18

Scale-up of malaria interventions over the last decade have yielded a significant reduction in malaria transmission and disease burden in sub-Saharan Africa. We estimated economic gradients in the distribution of these efforts and of their impacts within and across endemic countries. Using Demographic and Health Surveys we computed equity metrics to characterize the distribution of malaria interventions in 30 endemic countries proxying economic position with an asset-wealth index. Gradients were summarized in a concentration index, tabulated against level of coverage, and compared among interventions, across countries, and against respective trends over the period 2005-2015. There remain broad differences in coverage of malaria interventions and their distribution by wealth within and across countries. In most, economic gradients are lacking or favor the poorest for vector control; malaria services delivered through the formal healthcare sector are much less equitable. Scale-up of interventions in many countries improved access across the wealth continuum; in some, these efforts consistently prioritized the poorest. Expansions in control programs generally narrowed coverage gaps between economic strata; gradients persist in countries where growth was slower in the poorest quintile or where baseline inequality was large. Despite progress, malaria is consistently concentrated in the poorest, with the degree of inequality in burden far surpassing that expected given gradients in the distribution of interventions. Economic gradients in the distribution of interventions persist over time, limiting progress toward equity in malaria control. We found that, in countries with large baseline inequality in the distribution of interventions, even a small bias in expansion favoring the least poor yielded large gradients in intervention coverage while pro-poor growth failed to close the gap between the poorest and least poor. We demonstrated that dimensions of disadvantage compound for the poor; a lack of economic gradients in the distribution of malaria services does not translate to equity in coverage nor can it be interpreted to imply equity in distribution of risk or disease burden. Our analysis testifies to the progress made by countries in narrowing economic gradients in malaria interventions and highlights the scope for continued monitoring of programs with respect to equity.

Investigating the spatial distribution of water levels in the Mackenzie Delta using airborne LiDAR

USGS Publications Warehouse

Hopkinson, C.; Crasto, N.; Marsh, P.; Forbes, D.; Lesack, L.

2011-01-01

Airborne light detection and ranging (LiDAR) data were used to map water level (WL) and hydraulic gradients (??H/??x) in the Mackenzie Delta. The LiDAR WL data were validated against eight independent hydrometric gauge measurements and demonstrated mean offsets from - 0??22 to + 0??04 m (??< 0??11). LiDAR-based WL gradients could be estimated with confidence over channel lengths exceeding 5-10 km where the WL change exceeded local noise levels in the LiDAR data. For the entire Delta, the LiDAR sample coverage indicated a rate of change in longitudinal gradient (??2H/??x) of 5??5 ?? 10-10 m m-2; therefore offering a potential means to estimate average flood stage hydraulic gradient for areas of the Delta not sampled or monitored. In the Outer Delta, within-channel and terrain gradient measurements all returned a consistent estimate of - 1 ?? 10-5 m m-1, suggesting that this is a typical hydraulic gradient for the downstream end of the Delta. For short reaches (<10 km) of the Peel and Middle Channels in the middle of the Delta, significant and consistent hydraulic gradient estimates of - 5 ?? 10-5 m m-1 were observed. Evidence that hydraulic gradients can vary over short distances, however, was observed in the Peel Channel immediately upstream of Aklavik. A positive elevation anomaly (bulge) of > 0??1 m was observed at a channel constriction entering a meander bend, suggesting a localized modification of the channel hydraulics. Furthermore, water levels in the anabranch channels of the Peel River were almost 1 m higher than in Middle Channel of the Mackenzie River. This suggests: (i) the channels are elevated and have shallower bank heights in this part of the delta, leading to increased cross-delta and along-channel hydraulic gradients; and/or (ii) a proportion of the Peel River flow is lost to Middle Channel due to drainage across the delta through anastamosing channels. This study has demonstrated that airborne LiDAR data contain valuable information describing Arctic river delta water surface and hydraulic attributes that would be challenging to acquire by other means. ?? 2011 John Wiley & Sons, Ltd.

Automatic Segmentation of Granular Objects in Images: Combining Local Density Clustering and Gradient-Barrier Watershed

DTIC Science & Technology

2014-01-01

in applications, such as the recognition of biological cells [12,17], cell nuclei [8–10], colonies, and pollen [34,35], as well as nanoparticles [6... pollen image shown in Fig. 8, we see that two pollen grains on the upper-right are merged in the gradient-weighted distance trans- form watershed...10.1016/j.patcog.2013.11.004i images, pollen images, as well as physical nanoparticle images. This is only a small selection of this approach’s