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Sample records for griqualand west sequence

  1. A paleoweathering profile from Griqualand West, South Africa: evidence for a dramatic rise in atmospheric oxygen between 2.2 and 1.9 bybp

    NASA Technical Reports Server (NTRS)

    Holland, H. D.; Beukes, N. J.

    1990-01-01

    A core drilled near Wolhaarkop in Griqualand West, South Africa, intersected highly oxidized Kuruman Iron Formation below red beds of the Gamagara Formation. The lateral equivalents of the Kuruman Iron Formation in this drill hole consist largely of siderite, ankerite, magnetite, greenalite, and quartz. The oxidation of the Kuruman Iron Formation in WOL 2 occurred almost certainly during weathering prior to the deposition of the Gamagara Formation. The date of this weathering episode is bracketed between about 2.2 and 1.9 bybp by the age of the Ongeluk lavas in the Transvaal sequence below the unconformity and by the age of the Hartley lavas in the Olifantshoek Group above the unconformity. The ratio of iron to SiO2 in the several facies of the weathered Kuruman Iron Formation in WOL 2 is nearly the same as that in their unweathered equivalents. Since SiO2 loss during weathering was almost certainly minor, the similarity of the Fe/SiO2 ratio in the weathered and unweathered BIF indicates that nearly all the "FeO" in the Kuruman Iron Formation was oxidized and retained as FeO3 during weathering. Such a high degree of iron retention is best explained by an O2 content of the atmosphere > or = 0.03 atm at the time of weathering. Such an O2 pressure is very much greater than that suggested by the composition of paleosols developed on basalt > or = 2.2 bybp but is consistent with the highly oxidized nature of the 1.85 by Flin Flon paleosol. The new data suggest that PO2 rose dramatically from about 1 percent PAL (present atmospheric level) to > or = 15 percent PAL between 2.2 and 1.9 bybp.

  2. Microfossils from the Neoarchean Campbell Group, Griqualand West Sequence of the Transvaal Supergroup, and their paleoenvironmental and evolutionary implications

    NASA Technical Reports Server (NTRS)

    Altermann, W.; Schopf, J. W.

    1995-01-01

    The oldest filament- and colonial coccoid-containing microbial fossil assemblage now known is described here from drill core samples of stromatolitic cherty limestones of the Neoarchean, approximately 2600-Ma-old Campbell Group (Ghaap Plateau Dolomite, Lime Acres Member) obtained at Lime Acres, northern Cape Province, South Africa. The assemblage is biologically diverse, including entophysalidacean (Eoentophysalis sp.), probable chroococcacean (unnamed colonial coccoids), and oscillatoriacean cyanobacteria (Eomycetopsis cf. filiformis, and Siphonophycus transvaalensis), as well as filamentous fossil bacteria (Archaeotrichion sp.); filamentous possible microfossils (unnamed hematitic filaments) also occur. The Campbell Group microorganisms contributed to the formation of stratiform and domical to columnar stromatolitic reefs in shallow subtidal to intertidal environments of the Transvaal intracratonic sea. Although only moderately to poorly preserved, they provide new evidence regarding the paleoenvironmental setting of the Campbell Group sediments, extend the known time-range of entophysalidacean cyanobacteria by more than 400 million years, substantiate the antiquity and role in stromatolite formation of Archean oscillatoriacean cyanobacteria, and document the exceedingly slow (hypobradytelic) evolutionary rate characteristic of this early evolving prokaryotic lineage.

  3. The transvaal sequence: an overview

    NASA Astrophysics Data System (ADS)

    Eriksson, P. G.; Schweitzer, J. K.; Bosch, P. J. A.; Schereiber, U. M.; Van Deventer, J. L.; Hatton, C. J.

    1993-02-01

    The 15 000 m of relatively unmetamorphosed clastic and chemical sedimentary and volcanic rocks of the 2550-2050 Ma Transvaal Sequence as preserved within the Transvaal and correlated Griqualand West basins of South Africa, and in the Kanye basin of Botswana are described. Immature clastic sedimentary and largely andesitic volcanic rocks of the Wolkberg, Godwan and Buffelsfontein Groups and the Bloempoort and Wachteenbeetje Formations probably represent rift-related sequences of Ventersdorp age. The thin sandstones of the Black Reef Formation, developed at the base of both the Kanye and Transvaal basin successions and correlated with the basal Vryburg siltstones of the Griqualand West Sequence, are considered here to be the basal unit of the Transvaal Sequence. The Black Reef fluvial deposits grade up into the epeiric marine carbonates of the Malmani Subgroup. These stromatolitic dolomites and interdbedded cherts were laid down within a steepened carbonate ramp setting; transgressions from an initial Griqualand West compartment towards the northeast covered both the Kanye and Transvaal basins. Iron formations of the succeeding Penge Formation and Griqualand West correlates are envisaged as relatively shallow water shelf deposits within the carbonate platform model; siliceous breccias of the Kanye basin are interpreted as reflecting subaerial brecciation of exposed silica gels. The Duitschland Formation overlying the Penge iron formations is seen as a final, regressive clastic and chemical sedimentary deposits as the Malmani-Penge sea retreated from the Transvaal basin. The interbedded sandstones and mudstones of the uncomformity-bounded Pretoria Group probably represent a combination of alluvial fan and fluviodeltaic complexes debouching into the largely lacustrine Transvaal and Kanye basins. A strong glacial influence in the lower Pretoria Group is reflected in the correlated Makganyene diamicities of the Griqualand West Sequence. Sedimentation across all three

  4. Suggested revision for west mexican archeological sequences.

    PubMed

    Long, S V; Taylor, R E

    1966-12-16

    A review of the radiocarbon dates and published and unpublished archeological data from the West Mexican states of Sinaloa, Nayarit, Jalisco, and Colima has resulted in a revised tentative chronology for West Mexico.

  5. Models of hydrocarbon-bearing sequences of West Siberia

    SciTech Connect

    Mkrtohyan, O.M.; Gogonenkov, G.N.; Pavlov, N.E. )

    1991-03-01

    The West Siberian province located within a young plate (platform) is one of the largest in the world in size, reserves, number of unique fields, and hydrocarbon production. The southern, central, and western areas of the province are dominantly oil-bearing, the northern portion gas-bearing. All commercial reserves are within the Devonian-Cenomanian stratigraphic interval. Small accumulations in fractured-vuggy Devon-Carboniferous rocks are located in the zone of unconformity between this sequence and the Jurassic. The potential of the pre-plate middle Paleozoic, platform upper Paleozoic, and Triassic sequences recognized by seismic data in the northern, deepest, part of the plate is not known yet. Most hydrocarbon pools are located in the Jurassic-Cretaceous hydrocarbon-bearing sequences (HBS) within the plate cover. Structural-depositional oil accumulations with very changeable flow rates, a large stratigraphic-depositional accumulation in high permeability basal (quartz) reservoirs, and an oil-condensate and a multiple-pool structural gas field are discovered in the Middle Jurassic composed of potential continental coal-bearing and marine deposits. In most HBS, there is still a prospect of discovering new hydrocarbon pools including pools in more complex traps.

  6. Chloroplast genome sequencing analysis of Heterosigma akashiwo CCMP452 (West Atlantic) and NIES293 (West Pacific) strains

    PubMed Central

    Cattolico, Rose Ann; Jacobs, Michael A; Zhou, Yang; Chang, Jean; Duplessis, Melinda; Lybrand, Terry; McKay, John; Ong, Han Chuan; Sims, Elizabeth; Rocap, Gabrielle

    2008-01-01

    Background Heterokont algae form a monophyletic group within the stramenopile branch of the tree of life. These organisms display wide morphological diversity, ranging from minute unicells to massive, bladed forms. Surprisingly, chloroplast genome sequences are available only for diatoms, representing two (Coscinodiscophyceae and Bacillariophyceae) of approximately 18 classes of algae that comprise this taxonomic cluster. A universal challenge to chloroplast genome sequencing studies is the retrieval of highly purified DNA in quantities sufficient for analytical processing. To circumvent this problem, we have developed a simplified method for sequencing chloroplast genomes, using fosmids selected from a total cellular DNA library. The technique has been used to sequence chloroplast DNA of two Heterosigma akashiwo strains. This raphidophyte has served as a model system for studies of stramenopile chloroplast biogenesis and evolution. Results H. akashiwo strain CCMP452 (West Atlantic) chloroplast DNA is 160,149 bp in size with a 21,822-bp inverted repeat, whereas NIES293 (West Pacific) chloroplast DNA is 159,370 bp in size and has an inverted repeat of 21,665 bp. The fosmid cloning technique reveals that both strains contain an isomeric chloroplast DNA population resulting from an inversion of their single copy domains. Both strains contain multiple small inverted and tandem repeats, non-randomly distributed within the genomes. Although both CCMP452 and NIES293 chloroplast DNAs contains 197 genes, multiple nucleotide polymorphisms are present in both coding and intergenic regions. Several protein-coding genes contain large, in-frame inserts relative to orthologous genes in other plastids. These inserts are maintained in mRNA products. Two genes of interest in H. akashiwo, not previously reported in any chloroplast genome, include tyrC, a tyrosine recombinase, which we hypothesize may be a result of a lateral gene transfer event, and an unidentified 456 amino acid

  7. Fluviomarine sequences in the Lower Cretaceous of the west Netherlands basin

    SciTech Connect

    Van Der Zwan, K. ); Den Hartog Jager, D.; De Klerk, C. )

    1993-09-01

    The Lower Cretaceous of the West Netherlands Basin contains fluvial deposits of the Delfland Group, overlain by shallow marine sediments of the Rijnland Group. Sequence stratigraphy was applied to both succession, to study the nature of the transgression and to identify possible reservoir-seal pairs. A total of six depositional sequences has been identified using extensive new biostratigraphy and sedimentology. Three of the sequences contain fluvial sediments only, two are mixed fluvial and marine, and one is fully marine. The typical thickness per sequence is 200-400 m. Characteristic for the fluvial sequences is an overall fining-upward pattern. The sequence boundaries have been correlated on logs and on regional seismic. Each of the main depositional settings displays a characteristic seismic facies, which has been used to reconstruct the sedimentological facies distribution. The results demonstrate that the source direction of the Delfland Group was predominantly southeast, controlled by the tectonic grain. Strong thickness variations within the lowermost sequence indicate syndepositional rifting, which confined the main channel systems. The major intervening flood-plain shales correspond to base-level highstands, which implies they can be correlated regionally and may act as intraformational seals. The marine transgression entered the basin from the north during the late Valanginian. By the early Barremian, it covered the entire basin. Retrograding barrier sands from the transgressive systems tract are the best reservoirs within the marine succession. In both fluvial and marine settings, the use of sequence stratigraphy has enabled recognition of several types stratigraphic traps.

  8. Complete genomic sequence of a dengue type 2 virus from the French West Indies.

    PubMed

    Tolou, H; Couissinier-Paris, P; Mercier, V; Pisano, M R; de Lamballerie, X; de Micco, P; Durand, J P

    2000-10-14

    Severe forms of dengue fever, dengue haemorrhagic fever, and dengue shock syndrome, were not prominent in the Americas until the epidemic of Cuba in 1981. Since that time, they have spread to other countries in Central and South America, correlating with the spread of dengue type 2 viruses related to Southeast Asian strains. We report here the complete genomic sequence of a dengue type 2 virus isolated during the epidemic in La Martinique in 1998. This constitutes the first complete genetic characterization of a dengue virus strain from French West Indies, and also the first molecular identification in this region of a dengue 2 strain phylogenetically related to the emerging American type 2 dengue viruses.

  9. 3.2 Ga hydrothermal sedimentary sequence: DXCL drilling Project, West Pilbara, Australia

    NASA Astrophysics Data System (ADS)

    Kiyokawa, S.; Ito, T.; Ikehara, M.; Kitajima, F.; Yamaguchi, K. E.; Suganuma, Y.; Koge, S.; Sakamoto, R.; Naraoka, H.

    2008-12-01

    "Dixon Island" Cleaverville (DXCL) Drilling Project" was successfully performed during August 3 through 9, 2007, to obtain modern-weathering free fresh geologic samples of the 3.2 Ga Cleaverville Group at the Cleaverville Beach, west Pilbara, Australia. There are three cycles of volcanic and chemical sedimentary sequences; Lagoon, Dixon Island, Snapper Beach formations to the top. Each formation consist mainly of hydrothermal volcanic rocks (komatiite, rhyolite tuff and pillow basalt), organic-rich black chert, and iron-rich chert. We drilled three holes (CL1, CL2, and DX) that cover two formations (Snapper Beach and Dixon Island Formations of the Cleaverville Group). The drillcores CL1 (66.1m long) and CL2 (44.4m long) belong to the lower part of the Snapper Beach Formation, and the drillcore DX (100.15m long) belongs to the upper part of the Dixon Island Formation and Dixon Pillow Basalt. The total length of the drillcores is approximately 200m. We observed at least 40~50m deep uppermost sections that are visibly affected by modern weathering; however, the remaining portions are indeed "fresh," evidenced by preservation of fine grains/laminations and nodules of pyrite. All sequences of CL 1, CL 2, and DX are younging to southwest, which is identified by cross laminations and graded bed. The core of the DX consist very fine laminated black shale/gray chert/pyrite alteration. Well cyclic sedimentation is identified as annual event or hydrothermal activity cycles. The CL 1 and CL 2 cores are identified bedded black shale and fine grain volcanic sand layer. They contain finely laminated volcanic sandstone and very finely laminated black shale (mostly organic-rich and/or pyrite- rich) and gray-white chert. Total organic carbon (TOC) of the black shales are about 2-3%, and 13C is average 28°. TOC content is higher than that of average of content of black chert bed (0.1-0.2 %; Kiyokawa et al., 2006) in the Dixon Island Formation. These high organic content is higher

  10. Origin of West Indian populations of the geographically widespread boa Corallus enydris inferred from mitochondrial DNA sequences.

    PubMed

    Henderson, R W; Hedges, S B

    1995-03-01

    Corallus enydris (Serpentes: Boidae: Boinae) is an arboreal snake with an extremely wide mainland distribution from southern Costa Rica to southeastern Brazil and is one of two boine species that has invaded the Lesser Antilles (Grenada Bank and St. Vincent). Mitochondrial DNA sequences of samples from seven geographically disparate localities provided evidence of phylogenetic relationships. The monophyly of C. enydris is corroborated and a major dichotomy between northern samples (Panama and Trinidad) and southern samples (Guyana, Perú, southeastern Brazil) was found and corresponds to the two currently recognized subspecies. Unexpectedly, the two samples from the West Indies (southern Lesser Antilles) cluster with the southern rather than the geographically closer northern samples (e.g., Trinidad). The results imply a fairly recent Guianan-Amazonian origin of West Indian populations.

  11. Facies analysis and sequence stratigraphy of the Cenomanian-Turonian mixed siliciclastic-carbonate sediments in west Sinai, Egypt

    NASA Astrophysics Data System (ADS)

    Anan, Tarek I.

    2014-06-01

    The unconformity bounded Cenomanian-Turonian succession in west Sinai is subdivided into three formations: the Raha, Abu Qada, and Wata. These rock units were deposited in a mixed siliciclastic-carbonate system on a ramp setting. The studied ramp only includes inner and mid-ramp facies, whereas the outer ramp facies occurs in northern Sinai. Four sequence boundaries (SB) were recognized in the studied formations due to the presence of subaerial exposure or flooding events in the facies stacking pattern. The first and last sequence boundaries (SB1 and SB4) might be considered as Type 1 sequence boundary attributable to the presence of widespread erosion. It is difficult to determine type of the second and third sequence boundaries (SB2 and SB3) because of their minor unconformity and minimal erosion. Determination of systems tracts within the Wata Formation is debatable owing to the action of dolomitization that has destroyed both original components and sedimentary structures. The lowstand systems tracts of the recorded sequences are characterized by sandstones, siltstones, and sandy shales, while fossiliferous shale and limestone with oysters prevailed during sea level rise. The highstand systems tracts are generally characterized by shallow intertidal and subtidal deposits that are made up of abundant oyster wackestones with benthic foraminifera and ostracods.

  12. Thanetian transgressive-regressive sequences based on foraminiferal paleobathymetry at Gebel Matulla, west-central Sinai, Egypt

    NASA Astrophysics Data System (ADS)

    Farouk, Sherif; Elamri, Zaineb; El-Sorogy, Abdelbaset

    2016-09-01

    Qualitative and quantitative analysis of Thanetian foraminiferal assemblages at Gebel Matulla in west-central Sinai has been carried out. Three benthic foraminiferal assemblages are recorded from shallowest to deepest as Cibicidoides pseudoacutus, Angulogavelinella avnimelechi, Gavelinella danica witch evidences of fluctuations from middle neritic to upper bathyal environments. Changes in the foraminiferal population enabled us to classify the Thanetian succession into two fourth order transgressive-regressive (T-R) sequences. Three sequence boundaries are identified, at the Selandian/Thanetian (S/T) boundary, within the Thanetian succession, and the Paleocene/Eocene (P/E) boundary. It occurs at the top part of the maximum regression associated with major discontinuities and changes in depositional regimes as well as vertical facies changes. Broad correlation with eustatic records based upon integrated microplanktonic biostratigraphy suggests that the fluctuations of foraminiferal population were controlled by global sea-level changes.

  13. Draft Genome Sequence of Halomonas hydrothermalis MTCC 5445, Isolated from the West Coast of India.

    PubMed

    Bharadwaj Sv, Vamsi; Shrivastav, Anupama; Dubey, Sonam; Ghosh, Tonmoy; Paliwal, Chetan; Maurya, Rahulkumar; Mishra, Sandhya

    2015-01-01

    We announce here the draft genome sequence of Halomonas hydrothermalis MTCC 5445, a halophilic bacterium of the class Gammaproteobacteria. It was isolated from the sea coast of Aadri, Veraval, Gujarat, India. Its genome contains genes for polyhydroxybutyrate (PHB), a biodegradable polymer that can be used as a substitute for petroleum plastics. PMID:25593258

  14. Aftershocks triggered by fluid intrusion: Evidence for the aftershock sequence occurred 2014 in West Bohemia/Vogtland

    NASA Astrophysics Data System (ADS)

    Hainzl, S.; Fischer, T.; Čermáková, H.; Bachura, M.; Vlček, J.

    2016-04-01

    The West Bohemia/Vogtland region, central Europe, is well known for its repeating swarm activity. However, the latest activity in 2014, although spatially overlapping with previous swarm activity, consisted of three classical aftershock sequences triggered by ML3.5, 4.4, and 3.5 events. To decode the apparent system change from swarm-type to mainshock-aftershock characteristics, we have analyzed the details of the major ML4.4 sequence based on focal mechanisms and relocated earthquake data. Our analysis shows that the mainshock occurred with rotated mechanism in a step over region of the fault plane, unfavorably oriented to the regional stress field. Most of its intense aftershock activity occurred in-plane with classical characteristics such as (i) the maximum magnitude of the aftershocks is significantly less than the mainshock magnitude and (ii) the decay can be well fitted by the Omori-Utsu law. However, the absolute number of aftershocks and the fitted Omori-Utsu c and p parameters are much larger than for typical sequences. By means of the epidemic-type aftershock sequence model, we show that an additional aseismic source with an exponentially decaying strength triggered a large fraction of the aftershocks. Corresponding pore pressure simulations with an exponentially decreasing flow rate of the fluid source show a good agreement with the observed spatial migration front of the aftershocks extending approximately with log(t). Thus, we conclude that the mainshock opened fluid pathways from a finite fluid source into the fault plane explaining the unusual high rate of aftershocks, the migration patterns, and the exponential decrease of the aseismic signal.

  15. Sedimentology and sequence stratigraphy of upper Pleistocene carbonates of southeastern Barbardos, West Indies

    SciTech Connect

    Humphrey, J.D.; Kimbell, T.N. )

    1990-11-01

    Upper Pleistocene reef-associated carbonates of southeastern Barbados have been studied in outcrop and core. Reef terraces, formed during glacio-eustatic sea level highstands and subsequently uplifted, are characterized by thick and areally extensive sequences of allochthonous and autochthonous fore-reef calcarenites. Depositional textures are primarily packstones, and grainstones, wackestones, and coral floatstones are volumetrically less significant. Sediments are coarse- to fine-grained reef-derived allochems and micrite, and autochthonous benthic foraminifera and coralline red algae. Rates of sediment accumulation of fore-reef calcarenites range from about 1 to 4 m/1,000 yr. Although of relatively small scale, the carbonate terraces of southeastern Barbados provide excellent analogs for sequence stratigraphic concepts in carbonate settings. The terraces are primarily highstand systems tract deposits separated by type 1 unconformities. These highstand deposits are characterized by reef development and the progradation of fore-reef calcarenites. Extensive fore-reef deposits resulted from mechanical erosion of the reef framework on this high-energy, windward coastline. Type 1 unconformities are characterized by thin caliche layers developed during lowstand subaerial exposure. Thin basal transgressive systems tract deposits are characterized by incorporation of extraformational clasts derived from the underlying sequence during sea level rise. Slope-front erosion, vertical shift in the position of freshwater lens, and shift in the position of coastal onlap are all consequences of the interplay between eustasy and tectonics. These effects and the development of facies geometries on Barbados are primarily controlled by the glacio-eustatic component, inasmuch as rates of eustatic changes of sea level are at least two orders of magnitude greater than the maximum average rates of tectonic uplift. 12 figs.

  16. Repetitive reef to ooid sequences near leeward margin of Caicos Platform, British West Indies

    SciTech Connect

    Waltz, M.; Rossinsky, V.; Wanless, H.R.

    1987-05-01

    Drill core transects and outcrops near the leeward margin of the Caicos Platform, BWI, reveal repetitive (one Holocene and two Pleistocene) shallowing-upward sequences of either (a) reefal boundstones overlain by layered oolitic grainstones or (b) burrowed oolitic grainstones overlain by layered oolitic grainstones. Each sediment sequence is separated from the other by a calcrete exposure surface. A transect, perpendicular to the trend of an exposed Pleistocene barrier reef/ooid sand complex, shows two separate sediment packages of reefal boundstones and reef-derived skeletal packstones overlain by layered oolitic grainstones. The well-exposed upper package consists of a shallowing-upward barrier reef, which is immediately overlain by burrowed and cross-bedded oolitic grainstones, beach rock blocks, and coral rubble, capped by layered oolitic grainstones. Separated by an exposure horizon, the lowermost package consists of coral and skeletal sands overlain by layered oolitic grainstones. Cores from a transect in a non-reefal setting north of the barrier reef complex reveal highly burrowed oolitic grainstones capped by layered oolitic grainstones. As a Holocene example, immediately offshore of this transect, modern reefs and bioturbated oolitic grainstones are presently being buried beneath coral rubble, beach rock blocks, and prograding oolitic beaches. Deposition of the capping layered oolitic grainstones appears to occur during stable and falling sea levels. This co-occurrence of reefal sediment and ooid sands suggests that the two are not mutually exclusive and that reef-ooid succession is a reoccurring part of leeward margin platform margin-building.

  17. Genetic Characterization of Fasciola Isolates from West Azerbaijan Province Iran Based on ITS1 and ITS2 Sequence of Ribosomal DNA

    PubMed Central

    GALAVANI, Hossein; GHOLIZADEH, Saber; HAZRATI TAPPEH, Khosrow

    2016-01-01

    Background: Fascioliasis, caused by Fasciola hepatica and F. gigantica, has medical and economic importance in the world. Molecular approaches comparing traditional methods using for identification and characterization of Fasciola spp. are precise and reliable. The aims of current study were molecular characterization of Fasciola spp. in West Azerbaijan Province, Iran and then comparative analysis of them using GenBank sequences. Methods: A total number of 580 isolates were collected from different hosts in five cities of West Azerbaijan Province, in 2014 from 90 slaughtered cattle (n=50) and sheep (n=40). After morphological identification and DNA extraction, designing specific primer were used to amplification of ITS1, 5.8s and ITS2 regions, 50 samples were conducted to sequence, randomly. Result: Using morphometric characters 99.14% and 0.86% of isolates identified as F. hepatica and F. gigantica, respectively. PCR amplification of 1081 bp fragment and sequencing result showed 100% similarity with F. hepatica in ITS1 (428 bp), 5.8s (158 bp), and ITS2 (366 bp) regions. Sequence comparison among current study sequences and GenBank data showed 98% identity with 11 nucleotide mismatches. However, in phylogenetic tree F. hepatica sequences of West Azerbaijan Province, Iran, were in a close relationship with Iranian, Asian, and African isolates. Conclusions: Only F. hepatica species is distributed among sheep and cattle in West Azerbaijan Province Iran. However, 5 and 6 bp variation in ITS1 and ITS2 regions, respectively, is not enough to separate of Fasciola spp. Therefore, more studies are essential for designing new molecular markers to correct species identification. PMID:27095969

  18. The emerging tree of West Eurasian mtDNAs: a synthesis of control-region sequences and RFLPs.

    PubMed Central

    Macaulay, V; Richards, M; Hickey, E; Vega, E; Cruciani, F; Guida, V; Scozzari, R; Bonné-Tamir, B; Sykes, B; Torroni, A

    1999-01-01

    Variation in the human mitochondrial genome (mtDNA) is now routinely described and used to infer the histories of peoples, by means of one of two procedures, namely, the assaying of RFLPs throughout the genome and the sequencing of parts of the control region (CR). Using 95 samples from the Near East and northwest Caucasus, we present an analysis based on both systems, demonstrate their concordance, and, using additional available information, present the most refined phylogeny to date of west Eurasian mtDNA. We describe and apply a nomenclature for mtDNA clusters. Hypervariable nucleotides are identified, and the relative mutation rates of the two systems are evaluated. We point out where ambiguities remain. The identification of signature mutations for each cluster leads us to apply a hierarchical scheme for determining the cluster composition of a sample of Berber speakers, previously analyzed only for CR variation. We show that the main indigenous North African cluster is a sister group to the most ancient cluster of European mtDNAs, from which it diverged approximately 50,000 years ago. PMID:9915963

  19. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses.

    PubMed

    Lanciotti, R S; Kerst, A J

    2001-12-01

    The development and application of nucleic acid sequence-based amplification (NASBA) assays for the detection of West Nile (WN) and St. Louis encephalitis (SLE) viruses are reported. Two unique detection formats were developed for the NASBA assays: a postamplification detection step with a virus-specific internal capture probe and electrochemiluminescence (NASBA-ECL assay) and a real-time assay with 6-carboxyfluorescein-labeled virus-specific molecular beacon probes (NASBA-beacon assay). The sensitivities and specificities of these NASBA assays were compared to those of a newly described standard reverse transcription (RT)-PCR and TaqMan assays for SLE virus and to a previously published TaqMan assay for WN virus. The NASBA assays demonstrated exceptional sensitivities and specificities compared to those of virus isolation, the TaqMan assays, and standard RT-PCR, with the NASBA-beacon assay yielding results in less than 1 h. These assays should be of utility in the diagnostic laboratory to complement existing diagnostic testing methodologies and as a tool in conducting flavivirus surveillance in the United States.

  20. Full-Genome Sequence of a Neuroinvasive West Nile Virus Lineage 2 Strain from a Fatal Horse Infection in South Africa

    PubMed Central

    Mentoor, Juliet L. D.; Lubisi, Alison B.; Gerdes, Truuska; Human, Stacey; Williams, June H.

    2016-01-01

    We report here the complete genome sequence of a lineage 2 West Nile virus (WNV) strain that resulted in fatal neurological disease in a horse in South Africa. Several recent reports exist of neurological disease associated with lineage 2 WNV in humans and horses in South Africa and Europe; however, there are a lack of sequencing data from recent fatal cases in Southern Africa, where these strains likely originate. A better understanding of the genetic composition of highly neuroinvasive lineage 2 strains may facilitate the identification of putative genetic factors associated with increased virulence. PMID:27469963

  1. Full-Genome Sequence of a Neuroinvasive West Nile Virus Lineage 2 Strain from a Fatal Horse Infection in South Africa.

    PubMed

    Mentoor, Juliet L D; Lubisi, Alison B; Gerdes, Truuska; Human, Stacey; Williams, June H; Venter, Marietjie

    2016-01-01

    We report here the complete genome sequence of a lineage 2 West Nile virus (WNV) strain that resulted in fatal neurological disease in a horse in South Africa. Several recent reports exist of neurological disease associated with lineage 2 WNV in humans and horses in South Africa and Europe; however, there are a lack of sequencing data from recent fatal cases in Southern Africa, where these strains likely originate. A better understanding of the genetic composition of highly neuroinvasive lineage 2 strains may facilitate the identification of putative genetic factors associated with increased virulence. PMID:27469963

  2. Detection and sequencing of West Nile virus RNA from human urine and serum samples during the 2014 seasonal period.

    PubMed

    Nagy, Anna; Bán, Enikő; Nagy, Orsolya; Ferenczi, Emőke; Farkas, Ágnes; Bányai, Krisztián; Farkas, Szilvia; Takács, Mária

    2016-07-01

    West Nile virus, a widely distributed mosquito-borne flavivirus, is responsible for numerous animal and human infections in Europe, Africa and the Americas. In Hungary, the average number of human infections falls between 10 and 20 cases each year. The severity of clinically manifesting infections varies widely from the milder form of West Nile fever to West Nile neuroinvasive disease (WNND). In routine laboratory diagnosis of human West Nile virus infections, serological methods are mainly applied due to the limited duration of viremia. However, recent studies suggest that detection of West Nile virus RNA in urine samples may be useful as a molecular diagnostic test for these infections. The Hungarian National Reference Laboratory for Viral Zoonoses serologically confirmed eleven acute human infections during the 2014 seasonal period. In three patients with neurological symptoms, viral RNA was detected from both urine and serum specimens, albeit for a longer period and in higher copy numbers with urine. Phylogenetic analysis of the NS3 genomic region of three strains and the complete genome of one selected strain demonstrated that all three patients had lineage-2 West Nile virus infections. Our findings reaffirm the utility of viral RNA detection in urine as a molecular diagnostic procedure for diagnosis of West Nile virus infections. PMID:27038827

  3. Detection and sequencing of West Nile virus RNA from human urine and serum samples during the 2014 seasonal period.

    PubMed

    Nagy, Anna; Bán, Enikő; Nagy, Orsolya; Ferenczi, Emőke; Farkas, Ágnes; Bányai, Krisztián; Farkas, Szilvia; Takács, Mária

    2016-07-01

    West Nile virus, a widely distributed mosquito-borne flavivirus, is responsible for numerous animal and human infections in Europe, Africa and the Americas. In Hungary, the average number of human infections falls between 10 and 20 cases each year. The severity of clinically manifesting infections varies widely from the milder form of West Nile fever to West Nile neuroinvasive disease (WNND). In routine laboratory diagnosis of human West Nile virus infections, serological methods are mainly applied due to the limited duration of viremia. However, recent studies suggest that detection of West Nile virus RNA in urine samples may be useful as a molecular diagnostic test for these infections. The Hungarian National Reference Laboratory for Viral Zoonoses serologically confirmed eleven acute human infections during the 2014 seasonal period. In three patients with neurological symptoms, viral RNA was detected from both urine and serum specimens, albeit for a longer period and in higher copy numbers with urine. Phylogenetic analysis of the NS3 genomic region of three strains and the complete genome of one selected strain demonstrated that all three patients had lineage-2 West Nile virus infections. Our findings reaffirm the utility of viral RNA detection in urine as a molecular diagnostic procedure for diagnosis of West Nile virus infections.

  4. Cranial morphometrics and mitochondrial DNA sequences distinguish cryptic species of the longface emperor (Lethrinus olivaceus), an emblematic fish of Indo-West Pacific coral reefs.

    PubMed

    Borsa, Philippe; Hsiao, Dun-Ren; Carpenter, Kent E; Chen, Wei-Jen

    2013-10-01

    Range-wide morphometric variability (cranial measurements) and genetic variability (nucleotide sequences of the cytochrome b gene) were investigated in the longface emperor, Lethrinus olivaceus (Lethrinidae), an emblematic large predatory fish of Indo-West Pacific coral reefs. Two cranial morphotypes were observed, one present from the Indian Ocean to the Coral Triangle and the other one, from the Coral Triangle to the western Central Pacific. The two morphotypes are concordant with reciprocally monophyletic mitochondrial lineages separated by 9.5% net nucleotide distance. These results suggest an old evolutionary history for L. olivaceus, which consists of two distinct species (Lethrinus sp. A in the Indian Ocean and Coral Triangle, Lethrinus sp. B in the western Pacific Ocean), whose distribution ranges meet or overlap in the eastern part of the Coral Triangle, in Taiwan and in West Papua. Lethrinus sp. A comprises two distinct mitochondrial lineages separated by 1.7% net nucleotide distance, one exclusive to the populations from the Indian Ocean, the other exclusive to Coral Triangle populations. The latter observation might be explained by vicariance, whereby the two lineages have been isolated from one another on either side of the Sunda Shelf because of low sea level in the Pleistocene. To clarify the nomenclature of this species complex, we recommend sequencing a fragment of the cytochrome b gene of the holotypes of L. olivaceus and of its first junior synonyms L. rostratus and L. waigiensis. PMID:24246893

  5. Complete genome sequence of a lineage I peste des petits ruminants virus isolated in 1969 in west Africa.

    PubMed

    Dundon, William G; Yu, Daojin; Lô, Modou Moustapha; Loitsch, Angelika; Diop, Mariame; Diallo, Adama

    2015-01-01

    We report the complete genome sequence of a lineage I peste des petits ruminants virus (E32/1969) isolated in a Senegalese laboratory in 1969. This is the earliest peste des petits ruminants virus of any lineage sequenced to date and only the second lineage I virus available in public databases. PMID:25953180

  6. A conserved predicted pseudoknot in the NS2A-encoding sequence of West Nile and Japanese encephalitis flaviviruses suggests NS1' may derive from ribosomal frameshifting

    PubMed Central

    Firth, Andrew E; Atkins, John F

    2009-01-01

    Japanese encephalitis, West Nile, Usutu and Murray Valley encephalitis viruses form a tight subgroup within the larger Flavivirus genus. These viruses utilize a single-polyprotein expression strategy, resulting in ~10 mature proteins. Plotting the conservation at synonymous sites along the polyprotein coding sequence reveals strong conservation peaks at the very 5' end of the coding sequence, and also at the 5' end of the sequence encoding the NS2A protein. Such peaks are generally indicative of functionally important non-coding sequence elements. The second peak corresponds to a predicted stable pseudoknot structure whose biological importance is supported by compensatory mutations that preserve the structure. The pseudoknot is preceded by a conserved slippery heptanucleotide (Y CCU UUU), thus forming a classical stimulatory motif for -1 ribosomal frameshifting. We hypothesize, therefore, that the functional importance of the pseudoknot is to stimulate a portion of ribosomes to shift -1 nt into a short (45 codon), conserved, overlapping open reading frame, termed foo. Since cleavage at the NS1-NS2A boundary is known to require synthesis of NS2A in cis, the resulting transframe fusion protein is predicted to be NS1-NS2AN-term-FOO. We hypothesize that this may explain the origin of the previously identified NS1 'extension' protein in JEV-group flaviviruses, known as NS1'. PMID:19196463

  7. Next-generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model.

    PubMed

    Dridi, M; Rosseel, T; Orton, R; Johnson, P; Lecollinet, S; Muylkens, B; Lambrecht, B; Van Borm, S

    2015-10-01

    West Nile virus (WNV) occurs as a population of genetic variants (quasispecies) infecting a single animal. Previous low-resolution viral genetic diversity estimates in sampled wild birds and mosquitoes, and in multiple-passage adaptation studies in vivo or in cell culture, suggest that WNV genetic diversification is mostly limited to the mosquito vector. This study investigated genetic diversification of WNV in avian hosts during a single passage using next-generation sequencing. Wild-captured carrion crows were subcutaneously infected using a clonal Middle-East WNV. Blood samples were collected 2 and 4 days post-infection. A reverse-transcription (RT)-PCR approach was used to amplify the WNV genome directly from serum samples prior to next-generation sequencing resulting in an average depth of at least 700 ×  in each sample. Appropriate controls were sequenced to discriminate biologically relevant low-frequency variants from experimentally introduced errors. The WNV populations in the wild crows showed significant diversification away from the inoculum virus quasispecies structure. By contrast, WNV populations in intracerebrally infected day-old chickens did not diversify from that of the inoculum. Where previous studies concluded that WNV genetic diversification is only experimentally demonstrated in its permissive insect vector species, we have experimentally shown significant diversification of WNV populations in a wild bird reservoir species. PMID:26297666

  8. Next-generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model.

    PubMed

    Dridi, M; Rosseel, T; Orton, R; Johnson, P; Lecollinet, S; Muylkens, B; Lambrecht, B; Van Borm, S

    2015-10-01

    West Nile virus (WNV) occurs as a population of genetic variants (quasispecies) infecting a single animal. Previous low-resolution viral genetic diversity estimates in sampled wild birds and mosquitoes, and in multiple-passage adaptation studies in vivo or in cell culture, suggest that WNV genetic diversification is mostly limited to the mosquito vector. This study investigated genetic diversification of WNV in avian hosts during a single passage using next-generation sequencing. Wild-captured carrion crows were subcutaneously infected using a clonal Middle-East WNV. Blood samples were collected 2 and 4 days post-infection. A reverse-transcription (RT)-PCR approach was used to amplify the WNV genome directly from serum samples prior to next-generation sequencing resulting in an average depth of at least 700 ×  in each sample. Appropriate controls were sequenced to discriminate biologically relevant low-frequency variants from experimentally introduced errors. The WNV populations in the wild crows showed significant diversification away from the inoculum virus quasispecies structure. By contrast, WNV populations in intracerebrally infected day-old chickens did not diversify from that of the inoculum. Where previous studies concluded that WNV genetic diversification is only experimentally demonstrated in its permissive insect vector species, we have experimentally shown significant diversification of WNV populations in a wild bird reservoir species.

  9. Probing the Rare Biosphere of the North-West Mediterranean Sea: An Experiment with High Sequencing Effort.

    PubMed

    Crespo, Bibiana G; Wallhead, Philip J; Logares, Ramiro; Pedrós-Alió, Carlos

    2016-01-01

    High-throughput sequencing (HTS) techniques have suggested the existence of a wealth of species with very low relative abundance: the rare biosphere. We attempted to exhaustively map this rare biosphere in two water samples by performing an exceptionally deep pyrosequencing analysis (~500,000 final reads per sample). Species data were derived by a 97% identity criterion and various parametric distributions were fitted to the observed counts. Using the best-fitting Sichel distribution we estimate a total species richness of 1,568-1,669 (95% Credible Interval) and 5,027-5,196 for surface and deep water samples respectively, implying that 84-89% of the total richness in those two samples was sequenced, and we predict that a quadrupling of the present sequencing effort would suffice to observe 90% of the total richness in both samples. Comparing the HTS results with a culturing approach we found that most of the cultured taxa were not obtained by HTS, despite the high sequencing effort. Culturing therefore remains a useful tool for uncovering marine bacterial diversity, in addition to its other uses for studying the ecology of marine bacteria. PMID:27442429

  10. Probing the Rare Biosphere of the North-West Mediterranean Sea: An Experiment with High Sequencing Effort.

    PubMed

    Crespo, Bibiana G; Wallhead, Philip J; Logares, Ramiro; Pedrós-Alió, Carlos

    2016-01-01

    High-throughput sequencing (HTS) techniques have suggested the existence of a wealth of species with very low relative abundance: the rare biosphere. We attempted to exhaustively map this rare biosphere in two water samples by performing an exceptionally deep pyrosequencing analysis (~500,000 final reads per sample). Species data were derived by a 97% identity criterion and various parametric distributions were fitted to the observed counts. Using the best-fitting Sichel distribution we estimate a total species richness of 1,568-1,669 (95% Credible Interval) and 5,027-5,196 for surface and deep water samples respectively, implying that 84-89% of the total richness in those two samples was sequenced, and we predict that a quadrupling of the present sequencing effort would suffice to observe 90% of the total richness in both samples. Comparing the HTS results with a culturing approach we found that most of the cultured taxa were not obtained by HTS, despite the high sequencing effort. Culturing therefore remains a useful tool for uncovering marine bacterial diversity, in addition to its other uses for studying the ecology of marine bacteria.

  11. Probing the Rare Biosphere of the North-West Mediterranean Sea: An Experiment with High Sequencing Effort

    PubMed Central

    Crespo, Bibiana G.; Wallhead, Philip J.; Logares, Ramiro; Pedrós-Alió, Carlos

    2016-01-01

    High-throughput sequencing (HTS) techniques have suggested the existence of a wealth of species with very low relative abundance: the rare biosphere. We attempted to exhaustively map this rare biosphere in two water samples by performing an exceptionally deep pyrosequencing analysis (~500,000 final reads per sample). Species data were derived by a 97% identity criterion and various parametric distributions were fitted to the observed counts. Using the best-fitting Sichel distribution we estimate a total species richness of 1,568–1,669 (95% Credible Interval) and 5,027–5,196 for surface and deep water samples respectively, implying that 84–89% of the total richness in those two samples was sequenced, and we predict that a quadrupling of the present sequencing effort would suffice to observe 90% of the total richness in both samples. Comparing the HTS results with a culturing approach we found that most of the cultured taxa were not obtained by HTS, despite the high sequencing effort. Culturing therefore remains a useful tool for uncovering marine bacterial diversity, in addition to its other uses for studying the ecology of marine bacteria. PMID:27442429

  12. Seasonal dynamics of free-living tintinnid ciliate communities revealed by environmental sequences from the North-West Mediterranean Sea.

    PubMed

    Bachy, Charles; Moreira, David; Dolan, John R; López-García, Purificación

    2014-02-01

    The species-rich order Tintinnida is a group of planktonic ciliates ubiquitous in coastal marine waters, which can be well described using molecular estimates of diversity. We studied temporal changes of tintinnid diversity over 1 year in a coastal Mediterranean Sea location (Villefranche-sur-Mer, France) at five different depths (5, 25, 50, 100, and 160 m) and one additional year at 50 m depth by combining denaturing gel gradient electrophoresis (DGGE) community fingerprinting with direct PCR amplification, cloning and sequencing of small subunit (SSU), 5.8S, and large-subunit (LSU) rRNA genes together with the corresponding internal transcribed spacers (ITS). We amplified tintinnid sequences in all samples. All identified phylotypes were closely related to described species, showing that there is a good phylogenetic reference sequence data set allowing accurate estimation of tintinnid diversity in these waters. Tintinnid community composition exhibited marked seasonal changes. Surprisingly, the tintinnid SSU rDNA-based species composition did not show any clear relationship to measured environmental parameters (temperature, salinity, light, phytoplankton biomass). Nonetheless, the comparison of tintinnid community composition using UniFrac revealed three significant clusters of sequences grouping, respectively, samples collected in winter, autumn, and summer, leading to the hypothesis that seasonal effects on tintinnid community composition might be related to biotic parameters. In addition, phylogenetic trees based on the concatenated SSU + LSU rDNA and ITS sequences showed a better resolution than SSU rDNA alone to discriminate closely related species. PMID:24117776

  13. The 2004-2005 Les Saintes (French West Indies) seismic aftershock sequence observed with ocean bottom seismometers

    NASA Astrophysics Data System (ADS)

    Bazin, S.; Feuillet, N.; Duclos, C.; Crawford, W.; Nercessian, A.; Bengoubou-Valérius, M.; Beauducel, F.; Singh, S. C.

    2010-06-01

    On November 21, 2004 an Mw6.3 intraplate earthquake occurred at sea in the French Caribbean. The aftershock sequence continues to this day and is the most extensive sequence in a French territory in more than a century. We recorded aftershocks from day 25 to day 66 of this sequence, using a rapidly-deployed temporary array of ocean bottom seismometers (OBS). We invert P- and S-wave arrivals for a tomographic velocity model and improve aftershock locations. The velocity model shows anomalies related to tectonic and geologic structures beneath the Les Saintes graben. 3D relocated aftershocks outline faults whose scarps were identified as active in recent high-resolution marine data. The aftershocks distribution suggests that both the main November 21 event and its principal aftershock, on February 14, 2005, ruptured Roseau fault, which is the largest of the graben, extending from Dominica Island to the Les Saintes archipelago. Aftershocks cluster in the lower part of the Roseau fault plane (between 8 and 12.6 km depth) that did not rupture during the main event. Shallower aftershocks occur in the Roseau fault footwall, probably along smaller antithetic faults. We calculate a strong negative Vp anomaly, between 4 and 8 km depth, within the graben, along the Roseau fault plane. This low Vp anomaly is associated with a high Vp/Vs ratio and may reflect a strongly fracturated body filled with fluids. We infer from several types of observation that fault lubrication is the driving mechanism for this long-lasting aftershock sequence.

  14. Draft Genome Sequence of an Industrially Important Bacillus sp. from Mandarmani Coastal Waters in Midnapur District, West Bengal, India.

    PubMed

    Ray Chaudhuri, Shaon

    2016-01-01

    Reported here is the draft genome sequence of an amylase-, protease-, DNase-, oxidase-, gelatinase-, and catalase-producing, Gram-positive diplobacillus (Bacillus sp. SM1 strain MCC2138), which was isolated from marine coastal waters and has the ability to degum raw silk fabric as well as Ramie fiber. The genome comprises 1.76 Mb with a GC content of 34.5%. PMID:27540077

  15. Draft Genome Sequence of an Industrially Important Bacillus sp. from Mandarmani Coastal Waters in Midnapur District, West Bengal, India

    PubMed Central

    2016-01-01

    Reported here is the draft genome sequence of an amylase-, protease-, DNase-, oxidase-, gelatinase-, and catalase-producing, Gram-positive diplobacillus (Bacillus sp. SM1 strain MCC2138), which was isolated from marine coastal waters and has the ability to degum raw silk fabric as well as Ramie fiber. The genome comprises 1.76 Mb with a GC content of 34.5%. PMID:27540077

  16. The prevalence of badnaviruses in West African yams (Dioscorea cayenensis-rotundata) and evidence of endogenous pararetrovirus sequences in their genomes.

    PubMed

    Seal, Susan; Turaki, Aliyu; Muller, Emmanuelle; Kumar, P Lava; Kenyon, Lawrence; Filloux, Denis; Galzi, Serge; Lopez-Montes, Antonio; Iskra-Caruana, Marie-Line

    2014-06-24

    Yam (Dioscorea spp.) is an important vegetatively-propagated staple crop in West Africa. Viruses are pervasive in yam worldwide, decreasing growth and yield, as well as hindering the international movement of germplasm. Badnaviruses have been reported to be the most prevalent in yam, and genomes of some other badnaviruses are known to be integrated in their host plant species. However, it was not clear if a similar scenario occurs in Dioscorea yam. This study was conducted to verify the prevalence of badnaviruses, and determine if badnavirus genomes are integrated in the yam genome. Leaf samples (n=58) representing eight species of yam from global yam collections kept at CIRAD, France, and 127 samples of D. rotundata breeding lines (n=112) and landraces (n=15) at IITA, Nigeria, were screened using generic badnavirus PCR primers. Positive amplification of an expected ca. 579bp fragment, corresponding to a partial RT-RNaseH region, was detected in 47 (81%) of 58 samples analysed from CIRAD collections, and 100% of the 127 IITA D. rotundata samples. All the D. cayenensis and D. rotundata samples from the CIRAD and IITA collections tested PCR-positive, and sequencing of a selection of the PCR products confirmed they were typical of the genus Badnavirus. A comparison of serological and nucleic acid techniques was used to investigate whether the PCR-positives were sequences amplified from badnavirus particles or putative endogenous badnavirus sequences in the yam genome. Protein A sandwich-enzyme-linked immunosorbent assay (PAS-ELISA) with badnavirus polyclonal antisera detected cross-reacting viral particles in only 60% (92 of 153) of the CIRAD collection samples analysed, in contrast to the aforementioned 81% by PCR. Immunosorbent electron microscopy (ISEM) of virus preparations of a select set of 16 samples, representing different combinations of positive and negative PCR and PAS-ELISA results, identified bacilliform particles in 11 of these samples. Three PCR

  17. High Throughput Sequencing to Detect Differences in Methanotrophic Methylococcaceae and Methylocystaceae in Surface Peat, Forest Soil, and Sphagnum Moss in Cranesville Swamp Preserve, West Virginia, USA

    PubMed Central

    Lau, Evan; Nolan, Edward J.; Dillard, Zachary W.; Dague, Ryan D.; Semple, Amanda L.; Wentzell, Wendi L.

    2015-01-01

    Northern temperate forest soils and Sphagnum-dominated peatlands are a major source and sink of methane. In these ecosystems, methane is mainly oxidized by aerobic methanotrophic bacteria, which are typically found in aerated forest soils, surface peat, and Sphagnum moss. We contrasted methanotrophic bacterial diversity and abundances from the (i) organic horizon of forest soil; (ii) surface peat; and (iii) submerged Sphagnum moss from Cranesville Swamp Preserve, West Virginia, using multiplex sequencing of bacterial 16S rRNA (V3 region) gene amplicons. From ~1 million reads, >50,000 unique OTUs (Operational Taxonomic Units), 29 and 34 unique sequences were detected in the Methylococcaceae and Methylocystaceae, respectively, and 24 potential methanotrophs in the Beijerinckiaceae were also identified. Methylacidiphilum-like methanotrophs were not detected. Proteobacterial methanotrophic bacteria constitute <2% of microbiota in these environments, with the Methylocystaceae one to two orders of magnitude more abundant than the Methylococcaceae in all environments sampled. The Methylococcaceae are also less diverse in forest soil compared to the other two habitats. Nonmetric multidimensional scaling analyses indicated that the majority of methanotrophs from the Methylococcaceae and Methylocystaceae tend to occur in one habitat only (peat or Sphagnum moss) or co-occurred in both Sphagnum moss and peat. This study provides insights into the structure of methanotrophic communities in relationship to habitat type, and suggests that peat and Sphagnum moss can influence methanotroph community structure and biogeography. PMID:27682082

  18. Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing.

    PubMed

    Tsioris, Konstantinos; Gupta, Namita T; Ogunniyi, Adebola O; Zimnisky, Ross M; Qian, Feng; Yao, Yi; Wang, Xiaomei; Stern, Joel N H; Chari, Raj; Briggs, Adrian W; Clouser, Christopher R; Vigneault, Francois; Church, George M; Garcia, Melissa N; Murray, Kristy O; Montgomery, Ruth R; Kleinstein, Steven H; Love, J Christopher

    2015-12-01

    West Nile virus (WNV) infection is an emerging mosquito-borne disease that can lead to severe neurological illness and currently has no available treatment or vaccine. Using microengraving, an integrated single-cell analysis method, we analyzed a cohort of subjects infected with WNV - recently infected and post-convalescent subjects - and efficiently identified four novel WNV neutralizing antibodies. We also assessed the humoral response to WNV on a single-cell and repertoire level by integrating next generation sequencing (NGS) into our analysis. The results from single-cell analysis indicate persistence of WNV-specific memory B cells and antibody-secreting cells in post-convalescent subjects. These cells exhibited class-switched antibody isotypes. Furthermore, the results suggest that the antibody response itself does not predict the clinical severity of the disease (asymptomatic or symptomatic). Using the nucleotide coding sequences for WNV-specific antibodies derived from single cells, we revealed the ontogeny of expanded WNV-specific clones in the repertoires of recently infected subjects through NGS and bioinformatic analysis. This analysis also indicated that the humoral response to WNV did not depend on an anamnestic response, due to an unlikely previous exposure to the virus. The innovative and integrative approach presented here to analyze the evolution of neutralizing antibodies from natural infection on a single-cell and repertoire level can also be applied to vaccine studies, and could potentially aid the development of therapeutic antibodies and our basic understanding of other infectious diseases. PMID:26481611

  19. High Throughput Sequencing to Detect Differences in Methanotrophic Methylococcaceae and Methylocystaceae in Surface Peat, Forest Soil, and Sphagnum Moss in Cranesville Swamp Preserve, West Virginia, USA.

    PubMed

    Lau, Evan; Iv, Edward J Nolan; Dillard, Zachary W; Dague, Ryan D; Semple, Amanda L; Wentzell, Wendi L

    2015-04-02

    Northern temperate forest soils and Sphagnum-dominated peatlands are a major source and sink of methane. In these ecosystems, methane is mainly oxidized by aerobic methanotrophic bacteria, which are typically found in aerated forest soils, surface peat, and Sphagnum moss. We contrasted methanotrophic bacterial diversity and abundances from the (i) organic horizon of forest soil; (ii) surface peat; and (iii) submerged Sphagnum moss from Cranesville Swamp Preserve, West Virginia, using multiplex sequencing of bacterial 16S rRNA (V3 region) gene amplicons. From ~1 million reads, >50,000 unique OTUs (Operational Taxonomic Units), 29 and 34 unique sequences were detected in the Methylococcaceae and Methylocystaceae, respectively, and 24 potential methanotrophs in the Beijerinckiaceae were also identified. Methylacidiphilum-like methanotrophs were not detected. Proteobacterial methanotrophic bacteria constitute <2% of microbiota in these environments, with the Methylocystaceae one to two orders of magnitude more abundant than the Methylococcaceae in all environments sampled. The Methylococcaceae are also less diverse in forest soil compared to the other two habitats. Nonmetric multidimensional scaling analyses indicated that the majority of methanotrophs from the Methylococcaceae and Methylocystaceae tend to occur in one habitat only (peat or Sphagnum moss) or co-occurred in both Sphagnum moss and peat. This study provides insights into the structure of methanotrophic communities in relationship to habitat type, and suggests that peat and Sphagnum moss can influence methanotroph community structure and biogeography.

  20. High Throughput Sequencing to Detect Differences in Methanotrophic Methylococcaceae and Methylocystaceae in Surface Peat, Forest Soil, and Sphagnum Moss in Cranesville Swamp Preserve, West Virginia, USA

    PubMed Central

    Lau, Evan; Nolan, Edward J.; Dillard, Zachary W.; Dague, Ryan D.; Semple, Amanda L.; Wentzell, Wendi L.

    2015-01-01

    Northern temperate forest soils and Sphagnum-dominated peatlands are a major source and sink of methane. In these ecosystems, methane is mainly oxidized by aerobic methanotrophic bacteria, which are typically found in aerated forest soils, surface peat, and Sphagnum moss. We contrasted methanotrophic bacterial diversity and abundances from the (i) organic horizon of forest soil; (ii) surface peat; and (iii) submerged Sphagnum moss from Cranesville Swamp Preserve, West Virginia, using multiplex sequencing of bacterial 16S rRNA (V3 region) gene amplicons. From ~1 million reads, >50,000 unique OTUs (Operational Taxonomic Units), 29 and 34 unique sequences were detected in the Methylococcaceae and Methylocystaceae, respectively, and 24 potential methanotrophs in the Beijerinckiaceae were also identified. Methylacidiphilum-like methanotrophs were not detected. Proteobacterial methanotrophic bacteria constitute <2% of microbiota in these environments, with the Methylocystaceae one to two orders of magnitude more abundant than the Methylococcaceae in all environments sampled. The Methylococcaceae are also less diverse in forest soil compared to the other two habitats. Nonmetric multidimensional scaling analyses indicated that the majority of methanotrophs from the Methylococcaceae and Methylocystaceae tend to occur in one habitat only (peat or Sphagnum moss) or co-occurred in both Sphagnum moss and peat. This study provides insights into the structure of methanotrophic communities in relationship to habitat type, and suggests that peat and Sphagnum moss can influence methanotroph community structure and biogeography.

  1. Whole-exome sequencing identifies a variant of the mitochondrial MT-ND1 gene associated with epileptic encephalopathy: west syndrome evolving to Lennox-Gastaut syndrome.

    PubMed

    Delmiro, Aitor; Rivera, Henry; García-Silva, María Teresa; García-Consuegra, Inés; Martín-Hernández, Elena; Quijada-Fraile, Pilar; de Las Heras, Rogelio Simón; Moreno-Izquierdo, Ana; Martín, Miguel Ángel; Arenas, Joaquín; Martínez-Azorín, Francisco

    2013-12-01

    We describe a West syndrome (WS) patient with unidentified etiology that evolved to Lennox-Gastaut syndrome. The mitochondrial respiratory chain of the patient showed a simple complex I deficiency in fibroblasts. Whole-exome sequencing (WES) uncovered two heterozygous mutations in NDUFV2 gene that were reassigned to a pseudogene. With the WES data, it was possible to obtain whole mitochondrial DNA sequencing and to identify a heteroplasmic variant in the MT-ND1 (MTND1) gene (m.3946G>A, p.E214K). The expression of the gene in patient fibroblasts was not affected but the protein level was significantly reduced, suggesting that protein stability was affected by this mutation. The lower protein level also affected assembly of complex I and supercomplexes (I/III2 /IV and I/III2 ), leading to complex I deficiency. While ATP levels at steady state under stress conditions were not affected, the amount of ROS produced by complex I was significantly increased.

  2. Phylogenetic relationships of Western Mediterranean West Nile virus strains (1996-2010) using full-length genome sequences: single or multiple introductions?

    PubMed

    Sotelo, Elena; Fernández-Pinero, Jovita; Llorente, Francisco; Vázquez, Ana; Moreno, Ana; Agüero, Montserrat; Cordioli, Paolo; Tenorio, Antonio; Jiménez-Clavero, Miguel Ángel

    2011-11-01

    In recent years, West Nile virus (WNV) has re-emerged in the Western Mediterranean region. As a result, the number of complete WNV genome sequences available from this region has increased, allowing more detailed phylogenetic analyses, which may help to understand the evolutionary history of WNV circulating in the Western Mediterranean. To this aim, the present work describes six new complete WNV sequences from recent outbreaks and surveillance in Italy in 2008-2009 and in Spain in 2008 and 2010. Comparison with other sequences from different WNV clusters within lineage 1 (clade 1a) confirmed that all Western Mediterranean WNV isolates obtained since 1996 (except one from Tunisia, collected in 1997) cluster in a single monophyletic group (here called 'WMed' subtype). The analysis differentiated two subgroups within this subtype, which appear to have evolved from earlier WMed strains, suggesting a single introduction in the area, and further dissemination and evolution. Close similarities between WNV variants circulating in consecutive years, one in Spain, between 2007 and 2008, and another in Italy between 2008 and 2009, suggest that the virus possibly overwinters in Western Mediterranean sites. The NS3(249)-proline genotype, recently proposed as a virulence determinant for WNV, has arisen independently at least twice in the area. Overall, these results indicate that the frequent recurrence of outbreaks caused by phylogenetically homogeneous WNV in the Western Mediterranean since 1996 is consistent with a single introduction followed by viral persistence in endemic foci in the area, rather than resulting from independent introductions from exogenous endemic foci.

  3. The complete sequence of a West Nile virus lineage 2 strain detected in a Hyalomma marginatum marginatum tick collected from a song thrush (Turdus philomelos) in eastern Romania in 2013 revealed closest genetic relationship to strain Volgograd 2007.

    PubMed

    Kolodziejek, Jolanta; Marinov, Mihai; Kiss, Botond J; Alexe, Vasile; Nowotny, Norbert

    2014-01-01

    In this study the first complete sequence of the West Nile virus (WNV) lineage 2 strain currently circulating in Romania was determined. The virus was detected in a Hyalomma marginatum marginatum tick collected from a juvenile song thrush (Turdus philomelos) in the Romanian Danube Delta close to the city of Tulcea, end of August 2013. Our finding emphasizes the role of ticks in introduction and maintenance of WNV infections. Sequence analyses revealed close genetic relationship of the Romanian WNV strain to strain Reb_Volgograd_07_H, which was isolated from human brain tissue during an outbreak of West Nile neuroinvasive disease (WNND) in Russia in 2007. In 2010 the Eastern European lineage 2 WNV caused an outbreak of human WNND in Romania. Partial sequences from subsequent years demonstrated that this WNV strain became endemic in Eastern Europe and has been causing outbreaks of varying sizes in southern Russia since 2007 and in Romania since 2010.

  4. Evolution of the polyploid north-west Iberian Leucanthemum pluriflorum clan (Compositae, Anthemideae) based on plastid DNA sequence variation and AFLP fingerprinting

    PubMed Central

    Greiner, Roland; Vogt, Robert; Oberprieler, Christoph

    2013-01-01

    Background and Aims The genus Leucanthemum is a species-rich polyploid complex from southern and central Europe, comprising 41 species with ploidy ranging from 2x to 22x. The present contribution aims at reconstructing the evolutionary history of a geographically isolated species group (the L. pluriflorum clan) from the north-west Iberian Peninsula comprising the diploid L. pluriflorum, the tetraploids L. ircutianum subsp. pseudosylvaticum and L. × corunnense (a putative hybrid taxon based on crossing between L. pluriflorum and L. merinoi), and the hexaploids L. sylvaticum and L. merinoi. Methods Chromosome number variation (determined flow cytometrically) and sequence variation were analysed for two intergenic spacer regions on the plastid genome (psbA-trnH and trnC-petN) for individuals from 54 populations in combination with amplified fragment length polymorphism (AFLP) fingerprinting of 246 representative individuals from these populations. Key Results Plastid sequence data revealed that all surveyed members of the L. pluriflorum clan possess plastid haplotypes that are closely related to each other and distinctly separated from other Leucanthemum species. AFLP fingerprinting resulted in allopolyploid fragment patterns for most of the polyploid populations, except for the tetraploid L. × corunnense and a further tetraploid population in northern Galicia, which cluster with the diploids rather than with the other polyploids. In silico modelling of (auto)tetraploid AFLP genotypes further corroborates the allopolyploid nature of L. ircutianum subsp. pseudosylvaticum, L. sylvaticum and L. merinoi. Conclusions The present study provides evidence for recognizing one diploid (L. pluriflorum), one autotetraploid (L. corunnense), one allotetraploid (L. pseudosylvaticum) and one allohexaploid (L. sylvaticum with the two geographically and ecologically differentiated subspecies subsp. sylvaticum and subsp. merinoi) in the L. pluriflorum clan. It also has implications

  5. Revision of the Cretaceous fossil plant-assemblage from Gardeshwar (Gujarat, India): A conifer dominated floral association from an Upper Gondwana sequence on the West Coast of India

    NASA Astrophysics Data System (ADS)

    Jana, Brajendra Nath; King, Sarah C.; Hilton, Jason

    2013-09-01

    A small but diverse fossil plant assemblage from Gardeshwar in Gujarat Province of western India is reinvestigated, based on analysis of recently collected specimens that represent previously unrecognised taxa in combination with a critical review of previously reported taxa from the site. The assemblage is dominated by conifers including Brachyphyllum Brongniart, Elatocladus Halle, Pagiophyllum Heer, the cone Conites Sternberg, and ovulate scales of an araucarian conifer. Other plant groups are rare but include notable occurrences of the pteridophytes Lycopodites Lindley and Hutton and Gleichenia Smith, and the seed fern Sphenopteris (Brongniart) Sternberg. This assemblage is important as it represents the only datable fossils available from the Gardeshwar Formation and from the information presented we conclude it belongs to the Lower Cretaceous Allocladus-Brachyphyllum-Pagiophyllum floral biozone. The Gardeshwar assemblage association is unusual as it lacks the distinctive genus Allocladus but includes other taxa more typical of the Lower Cretaceous fern-dominated Weichselia-Onychiopsis-Gleichenia floral biozone, and may represent a transitional assemblage with characters of both biozones. However, this investigation highlights the lack of detailed stratigraphic analyses on the Lower Cretaceous sedimentary sequences of the west coast of India from which it remains uncertain if these two ‘biozones' are of different ages or whether they represent stratigraphically contemporaneous but ecologically distinct environments.

  6. Lithostratigraphic framework and correlation of the Neoproterozoic northern West African Craton passive margin sequence (Siroua-Zenaga-Bouazzer Elgraara Inliers, Central Anti-Atlas, Morocco): an integrated approach

    NASA Astrophysics Data System (ADS)

    Bouougri, El Hafid; Saquaque, Ali

    2004-06-01

    The Neoproterozoic volcano-sedimentary cover (˜0.8 Ga) deposited on the northern passive margin of the West African craton occurs in the central Anti-Atlas inliers and is a ˜2 km thick succession formally termed the Tizi n-Taghatine Group. The group comprises two sedimentary packages (lower and upper) separated by a middle volcanic unit. An integrated approach of lithostratigraphy, sedimentology, sequence stratigraphy and paleogeographic setting have been combined to provide well constrained correlation and lithostratigraphic organization. The Tizi n-Taghatine Group is formally subdivided into 12 Formations, 10 of which are defined in the lower sedimentary package while the middle volcanic unit and the upper sedimentary package constitute the two uppermost Formations. The lithostratigraphic organization and subdivision of the Tizi n-Taghatine Group constitutes a stratigraphic record of major paleogeographic settings and large cyclic changes in depositional system due to relative sea-level variations. The integrated approach of combination and comparison of various data provides significant information on the nature, origin and time significance of the formal unit boundaries. This leads to proposing a correlative scheme for the Tizi n-Taghatine Group across the Neoproterozoic central Anti-Atlas suture zone.

  7. Coastal uplift west of Algiers (Algeria): pre- and post-Messinian sequences of marine terraces and rasas and their associated drainage pattern

    NASA Astrophysics Data System (ADS)

    Authemayou, Christine; Pedoja, Kevin; Heddar, Aicha; Molliex, Stéphane; Boudiaf, Azzedine; Ghaleb, Bassam; Van Vliet Lanoe, Brigitte; Delcaillau, Bernard; Djellit, Hamou; Yelles, Karim; Nexer, Maelle

    2016-01-01

    The North Africa passive margin is affected by the ongoing convergence between the African and Eurasian plates. This convergence is responsible for coastal uplift, folding, and reverse faulting on new and reactivated faults on the margin. The active deformation is diffuse and thus rather difficult to locate precisely. We aim to determine how a coastal landscape evolve in this geodynamic setting and gain insights into active tectonics. More particularly, we evidence and quantify coastal uplift pattern of the Chenoua, Sahel, and Algiers reliefs (Algeria), using sequences of marine terraces and rasas and computing several morphometric indices from the drainage pattern. Upper and Middle Pleistocene uplift rates are obtained by fossil shoreline mapping and preliminary U/Th dating of associated coastal deposits. Extrapolation of these rates combined to analyses of sea-level referential data and spatial relationships between marine terraces/rasas and other geological markers lead us to tentatively propose an age for the highest coastal indicators (purported the oldest). Values of morphometric indices showing correlations with uplift rate allow us to analyze uplift variation on area devoid of coastal sequence. Geological and geomorphological data suggest that coastal uplift probably occurred since the Middle Miocene. It resulted in the emergence of the Algiers massif, followed by the Sahel ridge massif. The Sahel ridge has asymmetrically grown by folding from west to east and was affected by temporal variation of uplift. Compared to previous study, the location of the Sahel fold axis has been shifted offshore, near the coast. The Chenoua fault vertical motion does not offset significantly the coastal sequence. Mean apparent uplift rates and corrected uplift rates since 120 ka are globally steady all along the coast with a mean value of 0.055 ± 0.015 mm/year (apparent) and of 0.005 ± 0.045 mm/year (corrected for eustasy). Mean apparent coastal uplift rates between 120 and

  8. Stratigraphic Framework and Depositional Sequences in the Lower Silurian Regional Oil and Gas Accumulation, Appalachian Basin: From Licking County, Ohio, to Fayette County, West Virginia

    USGS Publications Warehouse

    Ryder, Robert T.

    2006-01-01

    wells drilled there since the late 1880s. However, new gas and local oil continues to be discovered in the deeper basin-center part (Zagorski, 1991; Pees, 1994; Petroleum Information Corporation, 1994). In general, only small quantities of gas have been produced from the Tuscarora Sandstone fields because of their generally poor reservoir quality and because of the low energy (Btu) content of the gas (Avary, 1996). Although fracture porosity is the dominant porosity type in the Tuscarora Sandstone gas reservoirs (Avary, 1996), there are several fields, such as Indian Creek, where intergranular porosity seems to be important (Bruner, 1983; Castle and Byrnes, 2005). In order to better understand the character and origin of the Lower Silurian regional oil and gas accumulation and its component parts, six cross sections were drawn through the Lower Silurian strata in parts of New York, Ohio, Pennsylvania, and West Virginia. The locations of all six cross sections are shown on sheet 2 (figs. 1 and 2) of this report. Each cross section shows the stratigraphic framework, depositional setting, sequence stratigraphy, and hydrocarbon-producing intervals of the Lower Silurian sandstone reservoirs and adjoining strata. Cross section F-F' presented here is about 215 mi long and trends northwestward, approximately normal to the depositional strike of the Lower Silurian sandstone system, and extends through large stretches of the basin-center and hybrid-conventional parts of the Lower Silurian regional oil and gas accumulation.

  9. Investigation of sea-level changes and shelf break prograding sequences during the Late Quaternary offshore of Kusadasi (West Anatolia) and surroundings by high resolution seismic methods

    NASA Astrophysics Data System (ADS)

    Gurcay, Savas; Cifci, Gunay; Dondurur, Derman; Okay, Seda; Atgin, Orhan; Ozel, Ozkan; Mert Kucuk, Hilmi

    2016-04-01

    High Resolution multi-channel seismic reflection and Chirp data were collected by K. Piri Reis, research vessel of Dokuz Eylül University, in the central Aegean coast of the West Anatolia by research cruises carried out in 2005 and 2008, respectively. Submarine stratigraphic and structural features of Sıǧacık Gulf, Kuşadası Gulf and surroundings were investigated under this survey. The data were processed and interpreted in SeisLab, D.E.U. Marine Sciences and Technology seismic laboratory. Thirteen distinct unconformities can be traced below the study area that separate thirteen progradational stacked paleo-delta sequences (Lob1-Lob13) on seismic profiles following and cutting each other. As a result of comparison with the oxygen isotopic stages (δ18), these deltas (Lob1-L13) were interpreted that they have been deposited during the sea-level lowstands within Pleistocene glacial stages. In the study area the basement surface which observed as the lowest unconformity surface of the seismic sections was called 'Acoustic Basement'. This basement which traced approximately all of the seismic sections has generally quite wavy surface and underlain the upper seismic units. It was observed that these seismic units which terminated their formation in Pleistocene (Lob1-Lob13) and Holocene period were cut and uplifted by acoustic basement, like an intrusion. These type deformations were interpreted as a result of magmatic intrusion into these upper seismic units occurred in Late Pleistocene and Holocene period. Tectonic and structural interpretation was carried out to constitute the submarine active tectonic map of the study area by correlated active faults identified on seismic sections. Submarine active tectonic map and, basement topography and sediment thickness map were correlated together to present the relationship between tectonic deformation and stratigraphy.

  10. Allele-specific qRT-PCR demonstrates superior detection of single nucleotide polymorphisms as genetic markers for West Nile virus compared to Luminex® and quantitative sequencing.

    PubMed

    Worwa, Gabriella; Andrade, Christy C; Thiemann, Tara C; Park, Bborie; Maharaj, Payal D; Anishchenko, Michael; Brault, Aaron C; Reisen, William K

    2014-01-01

    To enable in vivo and in vitro competitive fitness comparisons among West Nile viruses (WNV), three reference viruses were marked genetically by site-directed mutagenesis with five synonymous nucleotide substitutions in the envelope gene region of the genome. Phenotypic neutrality of the mutants was assessed experimentally by competitive replication in cell culture and genetic stability of the substituted nucleotides was confirmed by direct sequencing. Luminex(®) technology, quantitative sequencing and quantitative RT-PCR (qRT-PCR) were compared in regard to specificity, sensitivity and accuracy for quantitation of wildtype and genetically marked viruses in mixed samples based on RNA obtained from samples of known viral titers. Although Luminex(®) technology and quantitative sequencing provided semi-quantitative or qualitative measurements, a sequence-specific primer extension approach using a specific reverse primer set in singleplex qRT-PCR demonstrated the best quantitation and specificity in the detection of RNA from wildtype and mutant viruses.

  11. Hybrid channel flow-type mechanisms in the Greater Himalayan Sequence (West Nepal): new constraints from vorticity of flow and quartz petrofabric analyses.

    NASA Astrophysics Data System (ADS)

    Frassi, Chiara

    2016-04-01

    Three main tectono-metamorphic units are classically recognized along the Himalayan belt: the Lesser Himalayan (LH), the Greater Himalayan sequence (GHS) and the Tibetan Sedimentary sequence (TSS). The GHS may be interpreted as a low-viscosity tabular body of mid-crustal rocks extruded southward in Miocene times beneath the Tibetan plateau between two parallel and opposite-sense crustal-scale shear zones: the Main Central thrust at the base, and the South Tibetan Detachment system at the top. The pre-/syn-shearing mineral assemblage documented within these crustal-scale shear zones indicates that the metamorphic grade increases toward the core of the GHS producing an inverted and a normal thermal gradient respectively on the top and on the bottom of the slab. In addition, thermal profiles estimated using both petrology- and microstructures/fabrics-based thermometers indicate that the metamorphic isograds are condensed. Although horizontal extension and vorticity estimates collected across the GHS could be strongly biased by the criteria used to define the map position of the MCT, published vorticity data document general shear flow (1>Wk>0) within the slab with a pure-shear component of flow slightly predominant within the core of the GHS whereas the simple-shear component seems to dominate at the top of the slab. The lower boundary of the GHS records a general shear flow with a comparable contribution of simple and pure shearing. The associated crustal extrusion is compatible with Couette - Poiseuille velocity flow profile as assumed in crustal-scale channel flow-type models In this study, the quartz c-axis petrofabrics, vorticity and deformation-temperature studies are integrated with microstructures and metamorphic studies to individuate the location of the MCT and to document the spatial distribution of ductile deformation patterns across the lower portion of the GHS exposed in the Chaudabise river valley in western Nepal. My results indicate that the Main

  12. Complete Genome Sequence of the Highly Virulent Aeromonas hydrophila AL09-71 Isolated from Diseased Channel Catfish in West Alabama.

    PubMed

    Pridgeon, Julia W; Zhang, Dunhua; Zhang, Lee

    2014-05-22

    Aeromonas hydrophila AL09-71 was isolated from diseased channel catfish in west Alabama during a 2009 disease outbreak. The full genome of A. hydrophila AL09-71 is 5,023,861 bp. The availability of this genome will allow comparative genomics to identify genes involved in pathogenesis or immunogens for the purpose of vaccine development.

  13. Antibiotic sensitivity and sequence amplification patterns of genes in multidrug resistant enterobacteria isolates from processed foods in some West African countries.

    PubMed

    Owoseni, Abimbola Adetokunboh; Onilude, Abiodun Anthony

    2011-01-01

    Diarrhoea, dysentery and other diseases due to other enteric bacteria have reportedly been found to resist chemotherapeutic treatment in some West African communities with fatal consequences in some cases. This study was carried out to determine multidrug resistance patterns of Enterobacteria isolates from processed ready-to-eat foods. Indigenously processed food samples of different types were collected from two Francophone and two Anglophone countries in the West African sub-region during the wet and dry seasons of a sampling period of two years. Enterobacteria were isolated from the samples using standard techniques. Amplification of chromosomal DNA of the isolates using the Polymerase Chain Reaction was carried out. The results obtained were subjected to statistical analyses. All isolates showed resistance to cefuroxime (90.7%), nitrofurantoin (90.6%), augmentin (86.1%) and ampicillin (51.2%) while all were sensitive to gentamycin and ciprofloxacin. There was amplification indicating the presence of invA gene at a position of 240 bp. There was no amplification at all for the spvC gene in any of the isolates tested. Multidrug resistant enteric bacteria in these foods containing the invA gene could lead to infections with uncontrolled antibiotic use. The presence of enteric bacteria in the foods analyzed which provide undeniable evidence of the poor microbiological quality of these foods could form the basis of a useful databank in formulation of food-borne disease control and prevention strategies.

  14. Horizons West.

    ERIC Educational Resources Information Center

    Kitses, Jim

    The western is the most popular and enduring of Hollywood forms. It is one embodiment of a traditional theme in American culture: the West as both Garden of natural dignity and innocence and also as treacherous Desert resisting the gradual sweep of agrarian progress and community values. Westerns have in common: a) history, America's past; b)…

  15. Tectonic events, sequence stratigraphy and prediction of petroleum play elements in the Cretaceous and Tertiary of the northern Carnarvon Basin, north west shelf, Australia

    SciTech Connect

    Romine, K.K.; Durrant, J.D.

    1996-12-31

    The Carnarvon Basin is one of Australia`s most prolific oil and gas provinces. A recent Paleocene discovery has initiated a shift in exploration interest from traditional Jurassic/Triassic plays to the younger Cretaceous and Tertiary section. To improve play element prediction, a sequence stratigraphic study has been completed, utilizing newly acquired, regional high-resolution seismic data and 80 wells. The occurrence and distribution of the key play elements, reservoir, source and seal, is controlled by the interaction of tectonic subsidence, eustasy and paleogeography, with traps and migration pathways set up and modified by regional tectonic events. For example, a major rifting event commenced in the latest Kimmeridgian-Tithonian that resulted in structuring of older Jurassic sediments and initiation of seafloor spreading in the adjacent Cuvier-Gascoyne Abyssal Plain in the Valanginian. This event was accompanied by a dramatic fall in eustasy that initiated the deposition of high-quality reservoir sandstones of the Tithonian-Valanginian age Barrow Delta. The post-rift phase of thermal cooling and rapid subsidence resulted in transgression, accompanied by deposition of backstepping parasequences of the Mardie Greensand, a potential thief zone and reservoir, and culminated in maximum transgression and deposition of seal and source facies of the Muclerong Shale. The improved sequence stratigraphic framework established in this study provides a predictive tool for the development and assessment of new plays.

  16. Tectonic events, sequence stratigraphy and prediction of petroleum play elements in the Cretaceous and Tertiary of the northern Carnarvon Basin, north west shelf, Australia

    SciTech Connect

    Romine, K.K. ); Durrant, J.D. )

    1996-01-01

    The Carnarvon Basin is one of Australia's most prolific oil and gas provinces. A recent Paleocene discovery has initiated a shift in exploration interest from traditional Jurassic/Triassic plays to the younger Cretaceous and Tertiary section. To improve play element prediction, a sequence stratigraphic study has been completed, utilizing newly acquired, regional high-resolution seismic data and 80 wells. The occurrence and distribution of the key play elements, reservoir, source and seal, is controlled by the interaction of tectonic subsidence, eustasy and paleogeography, with traps and migration pathways set up and modified by regional tectonic events. For example, a major rifting event commenced in the latest Kimmeridgian-Tithonian that resulted in structuring of older Jurassic sediments and initiation of seafloor spreading in the adjacent Cuvier-Gascoyne Abyssal Plain in the Valanginian. This event was accompanied by a dramatic fall in eustasy that initiated the deposition of high-quality reservoir sandstones of the Tithonian-Valanginian age Barrow Delta. The post-rift phase of thermal cooling and rapid subsidence resulted in transgression, accompanied by deposition of backstepping parasequences of the Mardie Greensand, a potential thief zone and reservoir, and culminated in maximum transgression and deposition of seal and source facies of the Muclerong Shale. The improved sequence stratigraphic framework established in this study provides a predictive tool for the development and assessment of new plays.

  17. HLA-E coding and 3' untranslated region variability determined by next-generation sequencing in two West-African population samples.

    PubMed

    Castelli, Erick C; Mendes-Junior, Celso T; Sabbagh, Audrey; Porto, Iane O P; Garcia, André; Ramalho, Jaqueline; Lima, Thálitta H A; Massaro, Juliana D; Dias, Fabrício C; Collares, Cristhianna V A; Jamonneau, Vincent; Bucheton, Bruno; Camara, Mamadou; Donadi, Eduardo A

    2015-12-01

    HLA-E is a non-classical Human Leucocyte Antigen class I gene with immunomodulatory properties. Whereas HLA-E expression usually occurs at low levels, it is widely distributed amongst human tissues, has the ability to bind self and non-self antigens and to interact with NK cells and T lymphocytes, being important for immunosurveillance and also for fighting against infections. HLA-E is usually the most conserved locus among all class I genes. However, most of the previous studies evaluating HLA-E variability sequenced only a few exons or genotyped known polymorphisms. Here we report a strategy to evaluate HLA-E variability by next-generation sequencing (NGS) that might be used to other HLA loci and present the HLA-E haplotype diversity considering the segment encoding the entire HLA-E mRNA (including 5'UTR, introns and the 3'UTR) in two African population samples, Susu from Guinea-Conakry and Lobi from Burkina Faso. Our results indicate that (a) the HLA-E gene is indeed conserved, encoding mainly two different protein molecules; (b) Africans do present several unknown HLA-E alleles presenting synonymous mutations; (c) the HLA-E 3'UTR is quite polymorphic and (d) haplotypes in the HLA-E 3'UTR are in close association with HLA-E coding alleles. NGS has proved to be an important tool on data generation for future studies evaluating variability in non-classical MHC genes.

  18. The anisotropy of magnetic susceptibility (AMS) and paleomagnetic results from Lower Triassic sequence of West Spitsbergen Fold-and-Thrust Belt - case study.

    NASA Astrophysics Data System (ADS)

    Dudzisz, Katarzyna; Szaniawski, Rafał; Michalski, Krzysztof; Manby, Geoffrey

    2016-04-01

    Ninety-three oriented rock samples from 12 sites (ca. 450 specimens) located in the Lower Triassic Vardebukta Formation were analyzed. The samples were collected from the Hornsund - Sørkapp area in the southern part of West Spitsbergen Fold and Thrust Belt (WSFTB). The aim of this study was to test the anisotropy of magnetic susceptibility (AMS) method for the determination of tectonic paleostress pattern during the formation of WSFTB. The principal ferro- and paramagnetic minerals were also identified and their influence on the magnetic suceptibility was assessed. The NRM structure was determined in an attempt to link the remagnetization episodes with the well recognized tectono-thermal events related to WSFTB evolution. The results suggest that the magnetic susceptibility is controlled mainly by the paramagnetic minerals evidenced by the wide range of magnetic susceptibility values (20 - 400*10-6 SI). Only in one site, COND1, were the ferromagnetic minerals more dominant. A considerable variation in the shape of the AMS ellipsoids was noted. In eleven sites a normal magnetic fabric of sedimentary origin was detected which was associated with a relatively good clustering of the maximum AMS axes, caused by the tectonic strain. Samples with normal fabric reveal the presence of strong foliation parallel to the bedding plane. The orientation of the magnetic lineation, which indicates the maximum tectonic strain, approximates the regional structural NNW-SSE trend of the WSFTB. These results seem to support an orthogonal compression model for the formation of the WSFTB. Furthermore, the correspondence of the orientation of the maximum AMS axes with regional WSFTB structural trend is in conflict with interpretations assuming a strong strike - slip regime during WSFTB tectogenesis. The remaining two sites had mixed and inverted fabrics, the latter probably arising from the presence of iron-bearing carbonates in the samples. Preliminary palaeomagnetic results show that

  19. West Nile virus (WNV) genome RNAs with up to three adjacent mutations that disrupt long distance 5'-3' cyclization sequence basepairs are viable

    SciTech Connect

    Basu, Mausumi; Brinton, Margo A.

    2011-03-30

    Mosquito-borne flavivirus genomes contain conserved 5' and 3' cyclization sequences (CYC) that facilitate long distance RNA-RNA interactions. In previous studies, flavivirus replicon RNA replication was completely inhibited by single or multiple mismatching CYC nt substitutions. In the present study, full-length WNV genomes with one, two or three mismatching CYC substitutions showed reduced replication efficiencies but were viable and generated revertants with increased replication efficiency. Several different three adjacent mismatching CYC substitution mutant RNAs were rescued by a second site mutation that created an additional basepair (nts 147-10913) on the internal genomic side of the 5'-3' CYC. The finding that full-length genomes with up to three mismatching CYC mutations are viable and can be rescued by a single nt spontaneous mutation indicates that more than three adjacent CYC basepair substitutions would be required to increase the safety of vaccine genomes by creating mismatches in inter-genomic recombinants.

  20. Glossopteris Discovered in West Antarctica.

    PubMed

    Craddock, C; Bastien, T W; Rutford, R H; Anderson, J J

    1965-04-30

    Leaf impressions from the Polarstar Formation in the northern Ellsworth Mountains are the first Glossopteris and the oldest identifiable plant fossils reported from West Antarctica. Their occurrence in a thick, probably marine, geosynclinal sequence in close association with coals and probable glacial deposits increases the similarity between the late Paleozoic history of Antarctica and the other southern continents. PMID:17801935

  1. West Nile virus (WNV) genome RNAs with up to three adjacent mutations that disrupt long distance 5′-3′ cyclization sequence basepairs are viable

    PubMed Central

    Basu, Mausumi; Brinton, Margo A.

    2011-01-01

    Mosquito-borne flavivirus genomes contain conserved 5′ and 3′ cyclization sequences (CYC) that facilitate long distance RNA-RNA interactions. In previous studies, flavivirus replicon RNA replication was completely inhibited by single or multiple mismatching CYC nt substitutions. In the present study, full-length WNV genomes with one, two or three mismatching CYC substitutions showed reduced replication efficiencies but were viable and generated revertants with increased replication efficiency. Several different three adjacent mismatching CYC substitution mutant RNAs were rescued by a second site mutation that created an additional base pair (nts 147-10913) on the internal genomic side of the 5′-3′ CYC. The finding that full-length genomes with up to three mismatching CYC mutations are viable and can be rescued by a single nt spontaneous mutation indicates that more than three adjacent CYC basepair substitutions would be required to increase the safety of vaccine genomes by creating mismatches in inter-genomic recombinants. PMID:21292293

  2. West Africa

    NASA Technical Reports Server (NTRS)

    2002-01-01

    With its vast expanses of sand, framed by mountain ranges and exposed rock, northwestern Africa makes a pretty picture when viewed from above. This image was acquired by the Moderate-resolution Imaging Spectroradiometer (MODIS), flying aboard NASA's Terra spacecraft. The Canary Islands can be seen on the left side of the image just off Africa's Atlantic shore. The light brown expanse running through the northern two thirds of the image is the Sahara Desert. The desert runs up against the dark brown Haut Atlas mountain range of Morocco in the northwest, the Atlantic Ocean to the west and the semi-arid (light brown pixels) Sahelian region in the South. The Sahara, however, isn't staying put. Since the 1960s, the desert has been expanding into the Sahelian region at a rate of up to 6 kilometers per year. In the 1980s this desert expansion, combined with over cultivation of the Sahel, caused a major famine across west Africa. Over the summer months, strong winds pick up sands from the Sahara and blow them across the Atlantic as far west as North America, causing air pollution in Miami and damaging coral reefs in the Bahamas and the Florida Keys. The white outlines on the map represent country borders. Starting at the top-most portion of the map and working clockwise, the countries shown are Morocco, Western Sahara, Mauritania, Senegal, Mali, Burkina Fasso, Nigeria, Mali (again), and Algeria. Image by Reto Stockli, Robert Simmon, and Brian Montgomery, NASA Earth Observatory, based on data from MODIS

  3. West Nile virus

    MedlinePlus

    ... believe West Nile virus is spread when a mosquito bites an infected bird and then bites a person. ... avoid getting West Nile virus infection after a mosquito bite. People in good health generally do not develop ...

  4. America's Historic West.

    ERIC Educational Resources Information Center

    Beardsley, Donna A.

    Settlers who pushed west over the Great Divide to the shores of the Pacific Ocean found the American West to be an expanse of extreme differences in time, topography, and ways of life. This paper elaborates on several historic sites in the American West. The purpose of the paper is to introduce a series of places to the students and teachers of…

  5. Spectrophotometry of Comet West

    NASA Technical Reports Server (NTRS)

    Ahearn, M. F.; Hanisch, R. J.; Thurber, C. H.

    1980-01-01

    Postperihelion observations of Comet West (1975n = 1976 VI) have been made with a Fourier transform spectrometer at heliocentric distances from 0.57 to 1.68 AU. Measurements were made of the emission bands of C2, CN, C3, CH, and NH2, as well as the emission lines of Na D and forbidden (O I), and the flux in the continuum in nine different bandpasses. Several ratios of the band strengths of CN have been used to determine the two free parameters in the fluorescence equilibrium model of CN of Danks and Arpigny (1973). From the values of the parameters it is inferred that the vibrational transition probability for the ground electronic state is between 0.025 and 0.075 per sec and that the ratio of oscillator strengths between the (0-0) bands of the violet and red systems is between 25 and 30. When corrected for field-of-view effects, NH2 shows no systematic variation in abundance relative to C2 while CH shows a small increase. The cometary continuum is found to be slightly redder than the solar continuum, consistent with results for other bright, dusty comets. The equivalent width of the Delta u = 0 sequence of C2 shows a marked decrease at r(H) = 1.2 AU.

  6. 7. CLOSER OBLIQUE VIEW OF WEST TRUSS AND WEST SIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. CLOSER OBLIQUE VIEW OF WEST TRUSS AND WEST SIDE OF SOUTH ABUTMENT; VIEW TO NORTHEAST. - Mitchell's Mill Bridge, Spanning Winter's Run on Carrs Mill Road, west of Bel Air, Bel Air, Harford County, MD

  7. 110. WEST CHESTNUT STREET PAPTIST CHURCH AT 1725 WEST CHESTNUT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    110. WEST CHESTNUT STREET PAPTIST CHURCH AT 1725 WEST CHESTNUT STREET, WEST SIDE - Russell Neighborhood, Bounded by Congress & Esquire Alley, Fifteenth & Twenty-first Streets, Louisville, Jefferson County, KY

  8. 2. Building 5 west elevation, showing Building 4 west elevation ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Building 5 west elevation, showing Building 4 west elevation and stack associated with Building 3 to right. View looking SEE. - John & James Dobson Carpet Mill (West Parcel), Building No. 5, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  9. West wing, west elevation, seen entirety from the pasture at ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West wing, west elevation, seen entirety from the pasture at the west edge of state park property. (recreation of HABS No. CA-38-P152-1). - Vallejo Adobe, Adobe Road at Casa Grande, Petaluma, Sonoma County, CA

  10. 13. VIEW OF BRIDGE, LOOKING WEST FROM THE WEST TOWER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW OF BRIDGE, LOOKING WEST FROM THE WEST TOWER TO THE MAIN SUSPENSION CABLE WEST ANCHORAGE. February 1987 - Verde River Sheep Bridge, Spanning Verde River (Tonto National Forest), Cave Creek, Maricopa County, AZ

  11. Dominant simple-shear deformation during peak metamorphism for the lower portion of the Greater Himalayan Sequence in West Nepal: New implications for hybrid channel flow-type mechanisms in the Dolpo region

    NASA Astrophysics Data System (ADS)

    Frassi, Chiara

    2015-12-01

    I conducted new vorticity and deformation temperatures studies to test competing models of the exhumation of the mid-crustal rocks exposed in the Dolpo region (West Nepal). My results indicate that the Main Central Thrust is located ∼5 km structurally below the previous mapped locations. Deformation temperature increasing up structural section from ∼450 °C to ∼650 °C and overlap with peak metamorphic temperature indicating that penetrative shearing was responsible for the exhumation of the GHS occurred at "close" to peak metamorphic conditions. I interpreted the telescoping and the inversion of the paleo-isotherms at the base of the GHS as produced mainly by a sub-simple shearing (Wm = 0.88-1) pervasively distributed through the lower portion of the GHS. My results are consistent with hybrid channel flow-type models where the boundary between lower and upper portions of the GHS, broadly corresponding to the tectonometamorphic discontinuity recently documented in west Nepal, represents the limit between buried material, affected by dominant simple shearing, and exhumed material affected by a general flow dominates by pure shearing. This interpretation is consistent with the recent models suggesting the simultaneous operation of channel flow- and critical wedge-type processes at different structural depth.

  12. 5. EASTSIDE RESERVOIR, LOOKING WEST. WEST DAM UNDER CONSTRUCTION, QUARRIES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. EASTSIDE RESERVOIR, LOOKING WEST. WEST DAM UNDER CONSTRUCTION, QUARRIES TO LEFT MIDDLE GROUND OF PICTURE. - Eastside Reservoir, Diamond & Domenigoni Valleys, southwest of Hemet, Hemet, Riverside County, CA

  13. 38. SECOND FLOOR WEST SIDE APARTMENT WEST BEDROOM INTERIOR SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. SECOND FLOOR WEST SIDE APARTMENT WEST BEDROOM INTERIOR SHOWING PAIRED 6-LIGHT OVER 6-LIGHT DOUBLE-HUNG, WOOD-FRAME WINDOWS ON WEST WALL AND OPEN DOORWAY TO LIVING ROOM. VIEW TO WEST. - Lee Vining Creek Hydroelectric System, Triplex Cottage, Lee Vining Creek, Lee Vining, Mono County, CA

  14. West side of the north and west wings of the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West side of the north and west wings of the building - Fitzsimons General Hospital, Women's Army Corps Recreation & Administration Building, North Hickey Street, west side, 75 feet north of intersection of West Pennington Avenue & North Hickey Street, Aurora, Adams County, CO

  15. West and south sides of the west wing of the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West and south sides of the west wing of the building - Fitzsimons General Hospital, Women's Army Corps Recreation & Administration Building, North Hickey Street, west side, 75 feet north of intersection of West Pennington Avenue & North Hickey Street, Aurora, Adams County, CO

  16. Treatment of West syndrome.

    PubMed

    Sakakihara, Yoichi

    2011-03-01

    West syndrome is one of the most refractory epileptic syndromes in infancy, and many researchers have made great effort to find optimal treatment modalities for this syndrome. In this review, previous literature on optimal treatments of West syndrome and its refractory nature were briefly presented, followed by an introduction of recent publication of expert opinions from the US and Europe. An Asian expert opinion generated by a short questionnaire survey was then presented. It was shown that medically proven optimal treatment of West syndrome is not always the practical treatment of choice in Asian countries. Cost and geographical regions should also be taken into account in making practical choices for treatment of West syndrome. PMID:21196092

  17. WEST Physics Basis

    NASA Astrophysics Data System (ADS)

    Bourdelle, C.; Artaud, J. F.; Basiuk, V.; Bécoulet, M.; Brémond, S.; Bucalossi, J.; Bufferand, H.; Ciraolo, G.; Colas, L.; Corre, Y.; Courtois, X.; Decker, J.; Delpech, L.; Devynck, P.; Dif-Pradalier, G.; Doerner, R. P.; Douai, D.; Dumont, R.; Ekedahl, A.; Fedorczak, N.; Fenzi, C.; Firdaouss, M.; Garcia, J.; Ghendrih, P.; Gil, C.; Giruzzi, G.; Goniche, M.; Grisolia, C.; Grosman, A.; Guilhem, D.; Guirlet, R.; Gunn, J.; Hennequin, P.; Hillairet, J.; Hoang, T.; Imbeaux, F.; Ivanova-Stanik, I.; Joffrin, E.; Kallenbach, A.; Linke, J.; Loarer, T.; Lotte, P.; Maget, P.; Marandet, Y.; Mayoral, M. L.; Meyer, O.; Missirlian, M.; Mollard, P.; Monier-Garbet, P.; Moreau, P.; Nardon, E.; Pégourié, B.; Peysson, Y.; Sabot, R.; Saint-Laurent, F.; Schneider, M.; Travère, J. M.; Tsitrone, E.; Vartanian, S.; Vermare, L.; Yoshida, M.; Zagorski, R.; Contributors, JET

    2015-06-01

    With WEST (Tungsten Environment in Steady State Tokamak) (Bucalossi et al 2014 Fusion Eng. Des. 89 907-12), the Tore Supra facility and team expertise (Dumont et al 2014 Plasma Phys. Control. Fusion 56 075020) is used to pave the way towards ITER divertor procurement and operation. It consists in implementing a divertor configuration and installing ITER-like actively cooled tungsten monoblocks in the Tore Supra tokamak, taking full benefit of its unique long-pulse capability. WEST is a user facility platform, open to all ITER partners. This paper describes the physics basis of WEST: the estimated heat flux on the divertor target, the planned heating schemes, the expected behaviour of the L-H threshold and of the pedestal and the potential W sources. A series of operating scenarios has been modelled, showing that ITER-relevant heat fluxes on the divertor can be achieved in WEST long pulse H-mode plasmas.

  18. Mitogenome revealed multiple postdomestication genetic mixtures of West African sheep.

    PubMed

    Brahi, O H D; Xiang, H; Chen, X; Farougou, S; Zhao, X

    2015-10-01

    Notable diversity observed within African ovine breeds makes them of great interests, but limited studies on genetic origins and domestications remain poorly understood. Here, we investigate the evolutionary status of West African native breeds, Djallonke and Sahelian sheep using mitogenome sequencing. Compared with other ovine mitogenome sequences, West African sheep were revealed a Eurasian origin, and the initially tamed sheep breeds in West Africa have been genetically mixed with each other and mixed with European breeds. Worldwide domestic sheep is deemed the Eurasian origin and migrated west to Europe and Africa and east to the Far East, in which dispersed and received selection for acclimation to autochthonic environment independently and ultimately evolved into different native breeds, respectively. Our results contribute to the comprehensive understanding of the domestic sheep origin and reveal multiple postdomestication genetic amelioration processes.

  19. Histological and cytological evidence of viral infection and human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and normal tissue in the west of Scotland: evaluation of treatment policy

    PubMed Central

    Murdoch, J B; Cassidy, L J; Fletcher, K; Cordiner, J W; Macnab, J C M

    1988-01-01

    Biopsy samples from 27 patients referred to a colposcopy clinic in Glasgow for cervical abnormalities were assessed for the relations among colposcopic appearances, cytological and histological diagnosis, expression of papillomavirus antigen, and the presence of human papillomavirus (HPV) types 6, 11, 16, and 18 deoxyribonucleic acid (DNA) sequences. Specimens were from colposcopically abnormal areas of the transformation zone and from colposcopically apparently normal areas of the zone in the same patients (paired matched internal control tissue). All 27 women referred for abnormal smears had colposcopic abnormalities. HPV-16 or 18 DNA sequences were detected in 20 of the 27 colposcopically abnormal biopsy samples and 13 of the 27 paired normal samples. Twelve samples of colposcopically normal tissue contained histological evidence of viral infection but only four of these contained HPV DNA sequences. The other nine samples of colposcopically normal tissue which contained HPV DNA sequences were, however, histologically apparently normal. HPV-6 and 11 were not detected. Integration of the HPV-16 genome into the host chromosome was indicated in both cervical intraepithelial neoplasia and control tissues. In two thirds of the HPV DNA positive samples the histological grade was classed as normal, viral atypia, or cervical intraepithelial neoplasia grade 1. Papillomavirus antigen was detected in only six of the abnormal and three of the normal biopsy samples, and HPV DNA was detected in all of these. The detection of HPV DNA correlates well with a combination of histological and cytological evidence of viral infection (20 of 22 cases in this series). A poor correlation between the site on the cervix of histologically confirmed colposcopic abnormality and the presence of HPV DNA sequences implies that a cofactor other than HPV is required for preneoplastic disease to develop. A separate study in two further sets of biopsy samples examined the state of HPV DNA alone. The

  20. Coccinia intermedia – a new Cucurbitaceae species from West Africa

    PubMed Central

    Holstein, Norbert; Renner, Susanne S.

    2011-01-01

    Abstract Nuclear and plastid sequences from two individuals of a suspected new species of Coccinia from West Africa were added to an available molecular phylogeny for the remaining 27 species of the genus. Phylogenetic analyses of these data indicate the new species' monophyletic status and closest relatives. Based on four fertile collections, we here describe and illustrate Coccinia intermedia Holstein. We also provide a key to the Coccinia species of West Africa and map their distributions. PMID:22287923

  1. West Nile virus vaccine.

    PubMed

    Monath, T P; Arroyo, J; Miller, C; Guirakhoo, F

    2001-05-01

    Within the past 5 years, West Nile encephalitis has emerged as an important disease of humans and horses in Europe. In 1999, the disease appeared for the first time in the northeastern United States. West Nile virus (a mosquito-borne flavivirus) has flourished in the North American ecosystem and is expected to expand its geographic range. In this review, the rationale for a human and veterinary vaccine is presented and a novel approach for rapid development of a molecularly-defined, live, attenuated vaccine is described. The technology (ChimeriVax) is applicable to the development of vaccines against all flaviviruses, and products against Japanese encephalitis (a close relative of West Nile) and dengue are in or are nearing clinical trials, respectively. ChimeriVax vaccines utilize the safe and effective vaccine against the prototype flavivirus -yellow fever 17D- as a live vector. Infectious clone technology is used to replace the genes encoding the pre-membrane (prM) and envelope (E) protein of yellow fever 17D vaccine with the corresponding genes of the target virus (e.g., West Nile). The resulting chimeric virus contains the antigens responsible for protection against West Nile but retains the replication efficiency of yellow fever 17D. The ChimeriVax technology is well-suited to the rapid development of a West Nile vaccine, and clinical trials could begin as early as mid-2002. Other approaches to vaccine development are briefly reviewed. The aim of this brief review is to describe the features of West Nile encephalitis, a newly introduced infectious disease affecting humans, horses and wildlife in the United States; the rationale for rapid development of vaccines; and approaches to the development of vaccines against the disease.

  2. Composting moves west

    SciTech Connect

    Cotton, M.

    1996-05-01

    The art and science of composting has been applied to handling municipally generated organic wastes (particularly leaves, brush, and grass) in the Eastern US for many years, but now municipal composting can be really said to have gone west. Using methods farmers have been perfecting almost since the dawn of agriculture, municipalities in the US operate more than 4,000 composting sites across the country. Although a few municipal composting facilities have operated in the Western US for more than 30 years, the combination of cheap, plentiful landfill space, low population density, and extreme climate has prevented composting in the West from growing as fast as it has in other parts of the US. But continued growth in the West, combined with ambitious recycling goals in some Western states, have allowed composting to establish a major foothold as a practical solid waste management alternative.

  3. 51. Third Floor, Lake Forest, west center room, looking west, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    51. Third Floor, Lake Forest, west center room, looking west, part of original Forest Cottage as of 1901. - Lake Placid Club, Forest Wing, East side of Mirror Lake Drive, North of State Route 86 & Main, North Elba, Essex County, NY

  4. INTERIOR OF WEST SPAN LOOKING WEST (SHADOW OF VERTICAL LAPS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR OF WEST SPAN LOOKING WEST (SHADOW OF VERTICAL LAPS PLACED ON ZONE III; ASPHALT ZONE IX) - Honey Run Bridge, Spanning Butte Creek, bypassed section of Honey Run Road (originally Carr Hill Road), Paradise, Butte County, CA

  5. 3. VIEW TO EAST. DETAIL OF WEST FACE OF WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW TO EAST. DETAIL OF WEST FACE OF WEST STATION HOUSE. GREEK REVIVAL WINDOW ENTABLATURE. - Union Elevated Railroad, Madison-Wabash Avenue Station, Madison Steet & Wabash Avenue, Chicago, Cook County, IL

  6. 6. West side, details of west truss web and floorbeam ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. West side, details of west truss web and floor-beam bracing by steel plates and steel rod; looking northeast - Bridge No. 92101, Spanning Pike River at County Highway 373, Embarrass, St. Louis County, MN

  7. 35. DETAIL VIEW, WEST WINDOW IN WEST ELEVATION GABLE (NOTE: ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    35. DETAIL VIEW, WEST WINDOW IN WEST ELEVATION GABLE (NOTE: THE MOLDED STRINGCOURSE THAT PROJECTS FROM THE BASE OF THE FIRST FLOOR WINDOW ARCH AND VISIBLE WATERTABLE) - Kenworthy Hall, State Highway 14 (Greensboro Road), Marion, Perry County, AL

  8. GOES-West Shows U.S. West's Record Rainfall

    NASA Video Gallery

    A new time-lapse animation of data from NOAA's GOES-West satellite provides a good picture of why the U.S. West Coast continues to experience record rainfall. The new animation shows the movement o...

  9. 282. 183234 WEST MUHAMMAD ALI BOULEVARD, WEST SIDE (50511) TOWARD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    282. 1832-34 WEST MUHAMMAD ALI BOULEVARD, WEST SIDE (505-11) TOWARD NORTHEAST - Russell Neighborhood, Bounded by Congress & Esquire Alley, Fifteenth & Twenty-first Streets, Louisville, Jefferson County, KY

  10. 7. WEST PORTAL AND DECK VIEW, FROM WEST, SHOWING PORTAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. WEST PORTAL AND DECK VIEW, FROM WEST, SHOWING PORTAL CONFIGURATION AND LATERAL BRACING, STEEL MESH FLOOR, AND METAL RAILINGS - Glendale Road Bridge, Spanning Deep Creek Lake on Glendale Road, McHenry, Garrett County, MD

  11. 9. West elevation, west end of south wing wall, south ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. West elevation, west end of south wing wall, south abutment and south railing panel looking east - Western Maryland Railway Bridge, Spanning Maryland Route 51 at Spring Gap, Cumberland, Allegany County, MD

  12. 10. West elevation, west end of north wing wall, top ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. West elevation, west end of north wing wall, top of north abutment and oblique of railing panels looking northwest - Western Maryland Railway Bridge, Spanning Maryland Route 51 at Spring Gap, Cumberland, Allegany County, MD

  13. 5. DETAIL: Crib supporting bay, west of west gate. View ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. DETAIL: Crib supporting bay, west of west gate. View from the east. Rear of west breast wall is visible behind log timbers. Breast wall timbers are squared. - Wabash & Erie Canal, Lock No. 2, 8 miles east of Fort Wayne, adjacent to U.S. Route 24, New Haven, Allen County, IN

  14. 5. View West. West side and rear elevations of c. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View West. West side and rear elevations of c. 1890 first rear addition; partial north rear elevation of c. 1900 side ell addition; and north rear and west side elevation of final rear addition of c. 1940. - Vaughn Chevrolet Building, 101-109 East Main Street, Monongahela, Washington County, PA

  15. The West: Curriculum Package.

    ERIC Educational Resources Information Center

    Public Broadcasting Service, Alexandria, VA.

    This document consists of the printed components only of a PBS curriculum package intended to be used with the 9-videotape PBS documentary series entitled "The West." The complete curriculum package includes a teacher's guide, lesson plans, a student guide, audio tapes, a video index, and promotional poster. The teacher's guide and lesson plans…

  16. West Virginia baseline

    NASA Astrophysics Data System (ADS)

    Cardi, V. P.; Baer, C.; Graham, A.; Hall, T.; Rankin, D.; Sweet, T. J.

    1981-04-01

    Baseline information on West Virginia is provided. The topics covered are terrestrial ecology, aquatic ecology, geology and climatology, socioeconomics, and a legal analysis of institutional accountability. The hydrology, water quality, endangered species, and clean streams of five river basins are described.

  17. West Virginia and SREB

    ERIC Educational Resources Information Center

    Southern Regional Education Board (SREB), 2009

    2009-01-01

    The Southern Regional Education Board (SREB) is a nonprofit organization that works collaboratively with West Virginia and 15 other member states to improve education at every level--from pre-K to postdoctoral study--through many effective programs and initiatives. SREB's "Challenge to Lead" Goals for Education, which call for the region to lead…

  18. A West African Link

    ERIC Educational Resources Information Center

    Cook, Angela; Davies, Penny

    2003-01-01

    The authors visited Ghana in West Africa to strengthen a link established the previous year as part of Channel 4's "On the Line" project. The initial link established in 1999/2000 was between an all-age special school in Enfield and a similar school in Accra. Over the course of that year further partnerships were created between five UK schools…

  19. Glacial survival east and west of the 'Mekong-Salween Divide' in the Himalaya-Hengduan Mountains region as revealed by AFLPs and cpDNA sequence variation in Sinopodophyllum hexandrum (Berberidaceae).

    PubMed

    Li, Yong; Zhai, Sheng-Nan; Qiu, Ying-Xiong; Guo, Yan-Ping; Ge, Xue-Jun; Comes, Hans Peter

    2011-05-01

    Molecular phylogeographic studies have recently begun to elucidate how plant species from the Qinghai-Tibetan Plateau (QTP) and adjacent regions responded to the Quaternary climatic oscillations. In this regard, however, far less attention has been paid to the southern and south-eastern declivities of the QTP, i.e. the Himalaya-Hengduan Mountains (HHM) region. Here, we report a survey of amplified fragment length polymorphisms (AFLPs) and chloroplast DNA (cpDNA) sequence variation in the HHM endemic Sinopodophyllum hexandrum, a highly selfing alpine perennial herb with mainly gravity-dispersed berries (105 individuals, 19 localities). We specifically aimed to test a vicariant evolutionary hypothesis across the 'Mekong-Salween Divide', a known biogeographic and phytogeographic boundary of north-to-south trending river valleys separating the East Himalayas and Hengduan Mts. Both cpDNA and AFLPs identified two divergent phylogroups largely congruent with these mountain ranges. There was no genetic depauperation in the more strongly glaciated East Himalayas (AFLPs: H(E)=0.031; cpDNA: h(S)=0.133) compared to the mainly ice-free Hengduan Mts. (AFLPs: H(E)=0.037; cpDNA: h(S)=0.082), while population differentiation was consistently higher in the former region (AFLPs: Φ(ST)=0.522 vs. 0.312; cpDNA: Φ(ST)=0.785 vs. 0.417). Our results suggest that East Himalayan and Hengduan populations of S. hexandrum were once fragmented, persisted in situ during glacials in both areas, and have not merged again, except for a major instance of inter-lineage chloroplast capture identified at the MSD boundary. Our coalescent time estimate for all cpDNA haplotypes (c. 0.37-0.48 mya), together with paleogeological evidence, strongly rejects paleo-drainage formation as a mechanism underlying allopatric fragmentation, whereas mountain glaciers following the ridges of the MSD during glacials (and possible interglacials) could have been responsible. This study thus indicates an important role

  20. West and Central Africa.

    PubMed

    Lydie, N; Robinson, N J

    1998-01-01

    This article reviews scientific and other literature during the 1990s that links migration and mobility with the spread of sexually transmitted diseases (STDs), including HIV/AIDS. The focus is on key population groups linked to the spread of HIV and STDs in West and Central Africa: migrant laborers, truck drivers, itinerant traders, commercial sex workers (CSWs), and refugees. Countries with high emigration and immigration tend to have high levels of HIV infection, with the exception of Senegal. The main destination of immigrants are Senegal, Nigeria, and Cote d'Ivoire in West Africa and Cameroon, Congo, Gabon, and Congo in Central Africa. The risk of infection and the spread of HIV is variable among migrants. There is little in the literature that substantiates hypotheses about the strong association between migration and HIV-positive status. Information is needed on the duration, frequency of return visits, living conditions, sexual activities with multiple partners, and information before departure, along the routes, at final destination, and at the time of returns. Action-based research in five West African countries (Burkina Faso, Cote d'Ivoire, Mali, Niger, and Senegal) should produce results in late 1998. Comparable studies in Central Africa are unknown. Regional studies should be complemented by local studies. Prevention would benefit from studies on the relative size of these five population groups by geographic location.

  1. Drought in West Africa

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Drought settled over West Africa's Ivory Coast region when wet season rains came late in 2007. Instead of beginning in February, the rainy season didn't start until March, and steady rains didn't start until late March, said the Famine Early Warning System Network. Though the rain had started to alleviate the drought, vegetation was still depressed in parts of Cote d'Ivoire (Ivory Coast) between March 22 and April 6, 2007, when the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Terra satellite captured the data used to make this image. The image shows current vegetation conditions compared to average conditions recorded since 2000. Areas where plants are growing more slowly or more sparsely than average are brown, while areas where vegetation is denser than average are green. The brown tint that dominates the image indicates that plants through most of the country are more sparse than normal. Among the crops affected by the lack of rain was West Africa's cocoa crop. About 70 percent of the world's cocoa comes from West Africa, and Cote d'Ivoire is a top grower, said Reuters. Cocoa prices climbed as the crop fell short. Farmers called the drought the worst in living memory, Reuters said. The delay in rainfall also led to water shortages in parts of Cote d'Ivoire, according to the United Nations Office for the Coordination of Humanitarian Affairs.

  2. Genetic features of ancient West Siberian people of the Middle Ages, revealed by mitochondrial DNA haplogroup analysis.

    PubMed

    Sato, Takehiro; Razhev, Dmitry; Amano, Tetsuya; Masuda, Ryuichi

    2011-08-01

    In order to investigate the genetic features of ancient West Siberian people of the Middle Ages, we studied ancient DNA from bone remains excavated from two archeological sites in West Siberia: Saigatinsky 6 (eighth to eleventh centuries) and Zeleny Yar (thirteenth century). Polymerase chain reaction amplification and nucleotide sequencing of mitochondrial DNA (mtDNA) succeeded for 9 of 67 specimens examined, and the sequences were assigned to mtDNA haplogroups B4, C4, G2, H and U. This distribution pattern of mtDNA haplogroups in medieval West Siberian people was similar to those previously reported in modern populations living in West Siberia, such as the Mansi, Ket and Nganasan. Exact tests of population differentiation showed no significant differences between the medieval people and modern populations in West Siberia. The findings suggest that some medieval West Siberian people analyzed in the present study are included in direct ancestral lineages of modern populations native to West Siberia. PMID:21753768

  3. West Nile virus infection in killer whale, Texas, USA, 2007.

    PubMed

    St Leger, Judy; Wu, Guang; Anderson, Mark; Dalton, Les; Nilson, Erika; Wang, David

    2011-08-01

    In 2007, nonsuppurative encephalitis was identified in a killer whale at a Texas, USA, marine park. Panviral DNA microarray of brain tissue suggested West Nile virus (WNV); WNV was confirmed by reverse transcription PCR and sequencing. Immunohistochemistry demonstrated WNV antigen within neurons. WNV should be considered in cases of encephalitis in cetaceans. PMID:21801643

  4. Niobium content of soils from West Africa

    USGS Publications Warehouse

    Grimaldi, F.S.; Berger, I.A.

    1961-01-01

    Analysis of twenty lateritic soil samples from West Africa has shown them to contain an average 24 p.p.m. of niobium; four similar samples taken from within a few miles from a niobium deposit contain from 79 to 87 p.p.m. niobium. It has been shown that as the aluminum content of the soils increases, the following depletion sequence is obtained: Si > Nb > Al = Fe The data indicate that, in general, high enrichments of niobium are not to be expected in lateritic soils. ?? 1961.

  5. 42. WEST TO CIRCA 1900 JOINER ALONG WEST INTERIOR WALL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    42. WEST TO CIRCA 1900 JOINER ALONG WEST INTERIOR WALL OF FACTORY, BEHIND WHICH ARE MOUNTED JIGS AND PATTERNS FOR WINDMILL AND STEEL WATER TANK CONSTRUCTION, AS WELL AS THIN SHEET STEEL STENCILS USED FOR MARKING PRODUCTS WITH PAINT. - Kregel Windmill Company Factory, 1416 Central Avenue, Nebraska City, Otoe County, NE

  6. 29. VIEW SHOWING WEST APPROACH OF BRIDGE, LOOKING WEST. No ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    29. VIEW SHOWING WEST APPROACH OF BRIDGE, LOOKING WEST. No date. (The field welding was by the Teleweld Corporation of Chicago, with four Lincoln welders from Tuesday, April 19 to Monday, May 16, 1949. 528 man hours of welders' time) - Benton Street Bridge, Spanning Iowa River at Benton Street, Iowa City, Johnson County, IA

  7. 37. WEST REAR OF POWERHOUSE AND CAR BARN: West rear ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    37. WEST REAR OF POWERHOUSE AND CAR BARN: West rear of powerhouse and car barn, showing the turntable and tracks used to move cars in and out of the building's repair and storage area. - San Francisco Cable Railway, Washington & Mason Streets, San Francisco, San Francisco County, CA

  8. 18. VIEW OF CRUDE ORE BINS FROM WEST. WEST CRUDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. VIEW OF CRUDE ORE BINS FROM WEST. WEST CRUDE ORE BIN AND TRESTLE FROM TWO JOHNS TRAMLINE TO SOUTH, CRUDE ORE BIN IN FOREGROUND. MACHINE SHOP IN BACKGROUND. THE TRAM TO PORTLAND PASSED TO NORTH OF MACHINE SHOP. - Bald Mountain Gold Mill, Nevada Gulch at head of False Bottom Creek, Lead, Lawrence County, SD

  9. West Nile virus meningoencephalitis

    PubMed Central

    DeBiasi, Roberta L.; Tyler, Kenneth L.

    2013-01-01

    SUMMARY Since its first appearance in the US in 1999, West Nile virus (WNV) has emerged as the most common cause of epidemic meningoencephalitis in North America. In the 6 years following the 1999 outbreak, the geographic range and burden of the disease in birds, mosquitoes and humans has greatly expanded to include the 48 contiguous US and 7 Canadian provinces, as well as Mexico, the Caribbean islands and Colombia. WNV has shown an increasing propensity for neuroinvasive disease over the past decade, with varied presentations including meningitis, encephalitis and acute flaccid paralysis. Although neuroinvasive disease occurs in less than 1% of infected individuals, it is associated with high mortality. From 1999–2005, more than 8,000 cases of neuroinvasive WNV disease were reported in the US, resulting in over 780 deaths. In this review, we discuss epidemiology, risk factors, clinical features, diagnosis and prognosis of WNV meningoencephalitis, along with potential treatments. PMID:16932563

  10. High on 'West Spur'

    NASA Technical Reports Server (NTRS)

    2004-01-01

    A rock outcrop with a view of the surrounding landscape beckons NASA's Mars Exploration Rover Spirit on sol 203 (July 29, 2004) of its journey of exploration on the red planet. This view is a mosaic of images taken by the rover's navigation camera at a position labeled as Site 80, near the top of the 'West Spur' portion of the 'Columbia Hills.' Directly ahead are rock outcrops that scientists will examine for clues that might indicate the presence of water in the past. In the upper right-hand corner is the so-called 'sea of basalt,' consisting of lava flows that lapped onto the flanks of the hills. The view is toward the south. The field of view is approximately 170 degrees from right to left and is presented in a cylindrical projection with geometrical seam correction.

  11. West Candor Rocks

    NASA Technical Reports Server (NTRS)

    2005-01-01

    11 December 2005 This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) image shows light-toned, layered, sedimentary rock exposures in western Candor Chasma, part of the vast Valles Marineris trough system. Most of west Candor's interior includes exposures of layered rock with very few superimposed impact craters. The rock may be very ancient, but the lack of craters suggests that the erosion of these materials is on-going.

    Location near: 6.3oS, 76.0oW Image width: width: 3 km (1.9 mi) Illumination from: lower left Season: Southern Summer

  12. Ebola in West Africa.

    PubMed

    Raka, Lul; Guardo, Monica

    2015-03-15

    Ebola viral disease (EVD) is a severe and life-threatening disease. The current Ebola outbreak in West Africa entered its second year and is unprecedented because it is the largest one in history, involved urban centers and affected a large number of health care workers. It quickly escalated from medical into a humanitarian, social, economic, and security crisis. The primary pillars to prevent EVD are: early diagnosis, isolation of patients, contact tracing and monitoring, safe burials, infection prevention and control and social mobilization. The implementation of all these components was challenged in the field. Key lessons from this Ebola outbreak are that countries with weak health care systems can't withstand the major outbreaks; preparedness to treat the first confirmed cases is a national emergency; all control measures must be coordinated together and community engagement is the great factor to combat this disease.

  13. Dna Sequencing

    DOEpatents

    Tabor, Stanley; Richardson, Charles C.

    1995-04-25

    A method for sequencing a strand of DNA, including the steps off: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and at least three deoxyribonucleoside triphosphates in different amounts, under conditions in favoring primer extension to form nucleic acid fragments complementory to the DNA to be sequenced; labelling the nucleic and fragments; separating them and determining the position of the deoxyribonucleoside triphosphates by differences in the intensity of the labels, thereby to determine the DNA sequence.

  14. FAQ: West Nile Virus and Dead Birds

    MedlinePlus

    ... Education Public Service Videos West Nile Virus & Dead Birds Recommend on Facebook Tweet Share Compartir On this ... dead bird sightings to local authorities. How do birds get infected with West Nile virus? West Nile ...

  15. Micropaleontology and biostratigraphy of the coastal basins of West Africa

    SciTech Connect

    Kogbe, C.A.; Mehes, K.

    1986-01-01

    This book is intended to meet the need for a single volume on descriptive micropaleontology of West African microfauna assemblage which is different from that of the Boreal, Mediterranean, Pacific and Atlantic regions. The contents include: Preface. Introduction. Systematics: West African foraminifera species: Systematic classification and description. Glossary for the foraminifera. Selected references for the foraminifera. The ostracoda: Systematic classification and description. Glossary for the ostracoda. Selected references for the ostracoda. Stratigraphic Sequences of the West African Coastal Basins: General review. Angola-Cuanza basin. Congo. Gabon. Cameroun-Douala basin. Nigeria. Togo-Benin basin. Ghana. Ivory Coast. Senegal. Appendix: Brief classification of foraminifera. Paleo-ecology of the foraminifera. Testing of samples. Collection of samples. Preparation of samples. Preparation of thin sections. Storage of microfossils. Methods of examination. Index.

  16. 51. WEST ACROSS CLUTTER TO WEST WALL OF WELLSERVICE SHED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    51. WEST ACROSS CLUTTER TO WEST WALL OF WELL-SERVICE SHED ADDITION ON REAR OF FACTORY BUILDING. AT LOWER RIGHT FOREGROUND IS 1960S PICKUP TRUCK, THE LAST MOTOR VEHICLE USED IN WELL SERVICE BY THE KREGEL WINDMILL COMPANY. MOST OF THE OBJECTS VISIBLE IN THIS VIEW ARE CLUTTER NOT RELATED TO THE WELL SERVICE BUSINESS. - Kregel Windmill Company Factory, 1416 Central Avenue, Nebraska City, Otoe County, NE

  17. [Cases of West Nile fever in Novosibirsk region in 2004, and the genotyping of its viral pathogen].

    PubMed

    Ternovoĭ, V A; Protopopova, E V; Kononova, Iu V; Ol'khovikova, E A; Spiridonova, E A; Akopov, G D; Shestopalov, A M; Loktev, V B

    2007-01-01

    Three cases of West Nile fever were registered for the first time in Novosibirsk region in 2004. The diagnosis was confirmed by revealing IgG against West Nile virus (three cases) and viral RNA (two cases). Sequence analysis of fragments of E protein gene showed that the virus belonged to Ia genotype. PMID:17338376

  18. Sedimentary sequence evolution in a Foredeep basin: Eastern Venezuela

    SciTech Connect

    Bejarano, C.; Funes, D.; Sarzalho, S.; Audemard, F.; Flores, G.

    1996-08-01

    Well log-seismic sequence stratigraphy analysis in the Eastern Venezuela Foreland Basin leads to study of the evolution of sedimentary sequences onto the Cretaceous-Paleocene passive margin. This basin comprises two different foredeep sub-basins: The Guarico subbasin to the west, older, and the Maturin sub-basin to the east, younger. A foredeep switching between these two sub-basins is observed at 12.5 m.y. Seismic interpretation and well log sections across the study area show sedimentary sequences with transgressive sands and coastal onlaps to the east-southeast for the Guarico sub-basin, as well as truncations below the switching sequence (12.5 m.y.), and the Maturin sub-basin shows apparent coastal onlaps to the west-northwest, as well as a marine onlap (deeper water) in the west, where it starts to establish. Sequence stratigraphy analysis of these sequences with well logs allowed the study of the evolution of stratigraphic section from Paleocene to middle Miocene (68.0-12.0 m.y.). On the basis of well log patterns, the sequences were divided in regressive-transgressive-regressive sedimentary cycles caused by changes in relative sea level. Facies distributions were analyzed and the sequences were divided into simple sequences or sub- sequences of a greater frequencies than third order depositional sequences.

  19. GeoPowering the West

    SciTech Connect

    Not Available

    2007-02-01

    Summary brochure of GeoPowering the West (GPW) activities, and areas of technology transfer and market transformation. It also provides current contact information for key DOE and national laboratory staff representing the GPW program.

  20. Complete Genome Sequence of an Ebola Virus Isolate Imported from Sierra Leone to Germany Determined by Circle Sequencing

    PubMed Central

    Mengel, Jan Philipp; Lissin, Artur; Biedenkopf, Nadine; Schultze, Tilman; Mannala, Gopala Krishna; Schudt, Gordian; Kann, Gerrit; Wolf, Timo; Eickmann, Markus; Becker, Stephan

    2016-01-01

    We report here a complete genome sequence of Ebola virus Makona from a nonfatal patient sample that originated in Sierra Leone during the last Ebola virus outbreak in West Africa (species Zaire ebolavirus) using a highly accurate circle sequencing (Cir-seq) method. PMID:27795234

  1. 21 CFR 808.98 - West Virginia.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false West Virginia. 808.98 Section 808.98 Food and... and Local Exemptions § 808.98 West Virginia. (a) The following West Virginia medical device... has exempted them from preemption: West Virginia Code, sections 30-26-14 (b) and (c) and section...

  2. MSLICE Sequencing

    NASA Technical Reports Server (NTRS)

    Crockett, Thomas M.; Joswig, Joseph C.; Shams, Khawaja S.; Norris, Jeffrey S.; Morris, John R.

    2011-01-01

    MSLICE Sequencing is a graphical tool for writing sequences and integrating them into RML files, as well as for producing SCMF files for uplink. When operated in a testbed environment, it also supports uplinking these SCMF files to the testbed via Chill. This software features a free-form textural sequence editor featuring syntax coloring, automatic content assistance (including command and argument completion proposals), complete with types, value ranges, unites, and descriptions from the command dictionary that appear as they are typed. The sequence editor also has a "field mode" that allows tabbing between arguments and displays type/range/units/description for each argument as it is edited. Color-coded error and warning annotations on problematic tokens are included, as well as indications of problems that are not visible in the current scroll range. "Quick Fix" suggestions are made for resolving problems, and all the features afforded by modern source editors are also included such as copy/cut/paste, undo/redo, and a sophisticated find-and-replace system optionally using regular expressions. The software offers a full XML editor for RML files, which features syntax coloring, content assistance and problem annotations as above. There is a form-based, "detail view" that allows structured editing of command arguments and sequence parameters when preferred. The "project view" shows the user s "workspace" as a tree of "resources" (projects, folders, and files) that can subsequently be opened in editors by double-clicking. Files can be added, deleted, dragged-dropped/copied-pasted between folders or projects, and these operations are undoable and redoable. A "problems view" contains a tabular list of all problems in the current workspace. Double-clicking on any row in the table opens an editor for the appropriate sequence, scrolling to the specific line with the problem, and highlighting the problematic characters. From there, one can invoke "quick fix" as described

  3. Insertion Sequences

    PubMed Central

    Mahillon, Jacques; Chandler, Michael

    1998-01-01

    Insertion sequences (ISs) constitute an important component of most bacterial genomes. Over 500 individual ISs have been described in the literature to date, and many more are being discovered in the ongoing prokaryotic and eukaryotic genome-sequencing projects. The last 10 years have also seen some striking advances in our understanding of the transposition process itself. Not least of these has been the development of various in vitro transposition systems for both prokaryotic and eukaryotic elements and, for several of these, a detailed understanding of the transposition process at the chemical level. This review presents a general overview of the organization and function of insertion sequences of eubacterial, archaebacterial, and eukaryotic origins with particular emphasis on bacterial elements and on different aspects of the transposition mechanism. It also attempts to provide a framework for classification of these elements by assigning them to various families or groups. A total of 443 members of the collection have been grouped in 17 families based on combinations of the following criteria: (i) similarities in genetic organization (arrangement of open reading frames); (ii) marked identities or similarities in the enzymes which mediate the transposition reactions, the recombinases/transposases (Tpases); (iii) similar features of their ends (terminal IRs); and (iv) fate of the nucleotide sequence of their target sites (generation of a direct target duplication of determined length). A brief description of the mechanism(s) involved in the mobility of individual ISs in each family and of the structure-function relationships of the individual Tpases is included where available. PMID:9729608

  4. West African crude production diversifies

    SciTech Connect

    Aalund, L.

    1983-06-01

    Nigeria, with its seven crude-oil export streams, dominated West African production and accounted for over 70% of the depressed 1.8 million b/d output from the region last year. However, during the 1970s a flurry of new producing fields, primarily off the African coast, diversified production among a number of countries and touched off a wave of oil activity. The Journal takes a close look at the quality of West African oil in this installment of assays on world export crudes. This issue covers, in alphabetical order, Bonny Light (Nigeria) to Espoir (Ivory Coast). A following issue will wrap up West Africa by presenting assays on crudes from Forcados Blend (Nigeria) to Zaire Crude (Zaire).

  5. Serologic Evidence of West Nile Virus Transmission, Jamaica, West Indies

    PubMed Central

    Marra, Peter P.; Kramer, Laura D.

    2003-01-01

    In spring 2002, an intensive avian serosurvey was initiated in Jamaica, Puerto Rico, and Mexico. We collected >1,600 specimens from resident and nonresident neotropical migratory birds before their northerly migrations. Plaque reduction neutralization test results indicated specific neutralizing antibodies to West Nile virus in 11 resident species from Jamaica. PMID:12890329

  6. Primary Schooling in West Bengal

    ERIC Educational Resources Information Center

    Sen, Amartya

    2010-01-01

    With his Nobel Prize award money, Amartya Sen set up the Pratichi Trust which carries out research, advocacy and experimental projects in basic education, primary health care, and women's development in West Bengal and Bangladesh. Professor Sen himself took active interest in this work--helping set the agenda, looking at the evidence from…

  7. West Flank Downhole Temperature Data

    DOE Data Explorer

    Doug Blankenship

    2008-03-01

    Downhole temperature data for the three wells inside the West Flank FORGE footprint; 83-11, TCH 74-2 and TCH 48-11. TCH 74-2 and TCH 48-11 were both collected before 1990 and 83-11 was collected in 2009. The are compiled into one spreadsheet for ease of visualization.

  8. Improvisation in West African Musics.

    ERIC Educational Resources Information Center

    Locke, David

    1980-01-01

    Discussed is music of the sub-Sahara. Vocal, instrumental, and dance drumming from the Sudan Desert, the North Coast, East Horn, Central and West Africa, and contrapuntal yodeling of Pygmies is described. For African musicians, the ability to improvise, and creativity, are gifts from God. Includes selected readings and recordings. (KC)

  9. Articulating Success in West Virginia

    ERIC Educational Resources Information Center

    D'Antoni, Kathy J.; Coulson, Gene

    2008-01-01

    Two years in West Virginia, college filing cabinets were stacked high with articulation agreements. College faculty members were traveling to and from area high schools in an effort to develop and finalize articulation agreements; all part of the process. And to what end? Less than 1 percent of students in the state were taking advantage of the…

  10. The West Michigan Science Festival.

    ERIC Educational Resources Information Center

    Sheline, Mary Ann; Meyers, Karen

    1997-01-01

    Describes the West Michigan Science Festival which features a variety of interactive events in manufacturing, electronics, health care, engineering, and other fields. Brings together students, businesses, industries, and educators in a community celebration of science, mathematics, and technology. Discusses beginnings, business partners, events,…

  11. East Meets West: Mutual Images.

    ERIC Educational Resources Information Center

    Stanford Univ., CA. Center for Research in International Studies.

    This unit of study uses encounters between Japan and the West from earliest contact (16th century) to the present as a case study to help secondary students explore issues of cultural contact. The activities are appropriate for United States or world history courses as well as English, psychology, or sociology classes. The unit can be completed…

  12. B-WEST Mentorship Program.

    ERIC Educational Resources Information Center

    Taylor, Jennice, Comp.; Benedetti, Marie L., Ed.

    This program guide is intended for use by mentors of women who have completed the preemployment training component of the B-WEST (Building Workers Entering Skilled Trades) project and who are now entering a trade or technical occupation. The first section contains a definition of mentoring and mentor, protegee, and trainer profiles. Sections 2-6…

  13. 75 FR 17463 - Key West Bank, Key West, Florida; Notice of Appointment of Receiver

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-06

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF THE TREASURY Office of Thrift Supervision Key West Bank, Key West, Florida; Notice of Appointment of Receiver Notice... sole Receiver for Key West Bank, Key West, Florida, (OTS No. 14929) on March 26, 2010. Dated: March...

  14. 76 FR 68314 - Special Local Regulations; Key West World Championship, Atlantic Ocean; Key West, FL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-04

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations; Key West World Championship... Key West, Florida during the Key West World Championship, a series of high-speed boat races. The event..., Super Boat International Productions, Inc. is hosting the Key West World Championship, a series of...

  15. 33 CFR 110.189a - Key West Harbor, Key West, Fla., naval explosives anchorage area.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Key West Harbor, Key West, Fla..., DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.189a Key West Harbor, Key West, Fla., naval explosives anchorage area. (a) The anchorage ground. A circular area with...

  16. The Railroad and the Frontier West.

    ERIC Educational Resources Information Center

    Ewig, Rick

    1988-01-01

    Points out railroad building in the West as a cause rather than an effect of the rapid development of the area. Railroads hastened the demise of American Indian autonomy and their land base, brought permanent Anglo-American settlers to the West and provided a market relationship between East and West. (KO)

  17. 33 CFR 117.622 - West Bay

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false West Bay 117.622 Section 117.622 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.622 West Bay The draw of the West Bay Bridge, mile...

  18. 33 CFR 117.622 - West Bay.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false West Bay. 117.622 Section 117.622 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.622 West Bay. The draw of the West Bay Bridge, mile...

  19. 33 CFR 117.622 - West Bay

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false West Bay 117.622 Section 117.622 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.622 West Bay The draw of the West Bay Bridge, mile...

  20. 27 CFR 9.172 - West Elks.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false West Elks. 9.172 Section 9... TREASURY ALCOHOL AMERICAN VITICULTURAL AREAS Approved American Viticultural Areas § 9.172 West Elks. (a) Name. The name of the viticultural area described in this section is “West Elks.” (b) Approved...

  1. Phylogenetic Relationships of Southern African West Nile Virus Isolates

    PubMed Central

    Grobbelaar, Antoinette A.; Leman, Patricia A.; Anthony, Fiona S.; Gibson, Georgina V.F.; Swanepoel, Robert

    2002-01-01

    Phylogenetic relationships were examined for 29 southern African West Nile virus (formal name West Nile virus [WNV]) isolates from various sources in four countries from 1958 to 2001. In addition sequence data were retrieved from GenBank for another 23 WNV isolates and Kunjin and Japanese encephalitis viruses. All isolates belonged to two lineages. Lineage 1 isolates were from central and North Africa, Europe, Israel, and North America; lineage 2 isolates were from central and southern Africa and Madagascar. No strict correlation existed between grouping and source of virus isolate, pathogenicity, geographic distribution, or year of isolation. Some southern African isolates have been associated with encephalitis in a human, a horse, and a dog and with fatal hepatitis in a human and death of an ostrich chick. PMID:12141968

  2. Lyman-alpha observations of Comet West /1975n/

    NASA Technical Reports Server (NTRS)

    Opal, C. B.; Carruthers, G. R.

    1977-01-01

    The rate of hydrogen production of Comet West is studied through rocket observation of solar Lyman-alpha radiation resonantly scattered by the escaping hydrogen atoms. Two sets of Lyman-alpha exposure sequences are used to obtain computer-smoothed brightness contour (isophote) maps covering a density range of 100:1. A simple radial outflow model is applied to the contour maps to determine the rate of hydrogen production (3.2 by 10 to the 30th power atoms/sec.) Discrepancies between the observed shape of the outer isophotes and predicted models may be explained by optical depth effects, or by the presence of small pieces of the comet's nucleus distributed along the orbit. Hydrogen, carbon, and oxygen production for Comet West and Comet Kohoutek are compared; differences may be accounted for by variations in the composition or evolution of the two comets.

  3. 15. BUILDING 1: FOURTH FLOOR (West Section), TOP LEVEL OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. BUILDING 1: FOURTH FLOOR (West Section), TOP LEVEL OF TUBS, SOUTH AND WEST WALLS. OPEN METAL BREWER'S STAIR VISIBLE ALONG WEST WALL - Boston Beer Company, 225-249 West Second Street, South Boston, Suffolk County, MA

  4. 2. GENERAL STREET VIEW LOOKING WEST ON KENNEDY ST. FROM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. GENERAL STREET VIEW LOOKING WEST ON KENNEDY ST. FROM INTERSECTION OF SOUTH CONVENT AVE. AND WEST KENNEDY ST. - Barrio Libre, West Kennedy & West Seventeenth Streets, Meyer & Convent Avenues, Tucson, Pima County, AZ

  5. 3. GENERAL STREET VIEW LOOKING WEST ON KENNEDY FROM JUST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. GENERAL STREET VIEW LOOKING WEST ON KENNEDY FROM JUST SOUTH OF SOUTH CONVENT AVE. AND WEST KENNEDY ST. - Barrio Libre, West Kennedy & West Seventeenth Streets, Meyer & Convent Avenues, Tucson, Pima County, AZ

  6. 1. West portal of the mudshed abutting the west portal ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. West portal of the mudshed abutting the west portal of Tunnel 5, view to the northwest, 135mm lens. The flat-roofed reinforced concrete mudsheds, rocksheds, and snowsheds are a common feature of the Natron Cutoff over the summit of the Cascades. With the railroad located on a sidehill bench cut into the precipitous slopes, the sheds protect the track from rock and mud slides, as well as from avalanches. With a solid wall on the uphill side and a series of columns on the downhill side, they form a gallery-like effect from within. This mudshed was built concurrent with the tunnel, in 1927. Though none of the mudsheds on the line are scheduled to be modified, this shed was documented as an integral element of Tunnel 5. - Southern Pacific Railroad Natron Cutoff, Tunnel 5, Milepost 545.2, McCredie Springs, Lane County, OR

  7. Tectonosedimentary history of the sedimentary basins in northern west Siberia

    SciTech Connect

    Kunin, N.Ya.; Segalovich, I.E. )

    1993-09-01

    Sedimentary basins of northern west Siberia belong to the Arctic tectonosedimentary province. This basin evolved dissimilarly compared to those in the Urengoy and more southern areas, which resulted in substantial differences in the geologic characteristics. Seismic surveys indicate that the basement surface in northern west Siberia occurs at great depths, in places exceeding 15 km. The depressions of the basement surfaces are filled with the thick Paleozoic and Mesozoic sequences. The paper discussed the results of seismostratigraphic analysis of more than 13,000 km of regional common-depth-point profiles. These profiles identified systems of east-west-trending and isometric structures in the region. Some of the structures are buried; others are mapped in the upper horizons of the sedimentary cover and decrease in magnitude with depth. Cretaceous marine sediments that were deposited under deep-water conditions and did not compensate for the tectonic subsidence are widely present in the region. Noncompensated sedimentation was the longest from the Late Jurassic to the Hauterivian-Barremian on the Gydan peninsula and in adjacent areas. The Jurassic section is dominate by ingressive marine sediments. Sediments that did not compensate for tectonic subsidence widely occurred in the Early Jurassic and resulted in deposition of petroleum source rocks. Triassic and Jurassic strata occur conformable in most of northern west Siberia. Significant deformation of the Triassic sediments are identified in the periphery of the Triassic marine basin. This indicates that surrounding structures were thrust against northern west Siberia at the Triassic and Jurassic time boundary. Isometric structures of high magnitude were formed during the Paleozoic structure stage and these structures continued to grow through the Triassic and Jurassic. These and other results of seismostratigraphic analysis suggest the high oil potential of the region.

  8. The sequence of sequencers: The history of sequencing DNA.

    PubMed

    Heather, James M; Chain, Benjamin

    2016-01-01

    Determining the order of nucleic acid residues in biological samples is an integral component of a wide variety of research applications. Over the last fifty years large numbers of researchers have applied themselves to the production of techniques and technologies to facilitate this feat, sequencing DNA and RNA molecules. This time-scale has witnessed tremendous changes, moving from sequencing short oligonucleotides to millions of bases, from struggling towards the deduction of the coding sequence of a single gene to rapid and widely available whole genome sequencing. This article traverses those years, iterating through the different generations of sequencing technology, highlighting some of the key discoveries, researchers, and sequences along the way.

  9. West Nile Virus in California

    PubMed Central

    Lothrop, Hugh; Chiles, Robert; Madon, Minoo; Cossen, Cynthia; Woods, Leslie; Husted, Stan; Kramer, Vicki; Edman, John

    2004-01-01

    West Nile virus (WNV) was first isolated in California during July 2003 from a pool of Culex tarsalis collected near El Centro, Imperial County. WNV transmission then increased and spread in Imperial and Coachella Valleys, where it was tracked by isolation from pools of Cx. tarsalis, seroconversions in sentinel chickens, and seroprevalence in free-ranging birds. WNV then dispersed to the city of Riverside, Riverside County, and to the Whittier Dam area of Los Angeles County, where it was detected in dead birds and pools of Cx. pipiens quinquefasciatus. By October, WNV was detected in dead birds collected from riparian corridors in Los Angeles, west to Long Beach, and through inland valleys south from Riverside to San Diego County. WNV was reported concurrently from Arizona in mid-August and from Baja, Mexico, in mid-November. Possible mechanisms for virus introduction, amplification, and dispersal are discussed. PMID:15496236

  10. Lower Palaeozoic of North-Western and West-Central Africa

    SciTech Connect

    Holland, C.H.

    1985-01-01

    The first compilation in English of the stratigraphy, palaeontology, sedimentology, palaeogeography, and palaeoclimatology of the Lower Palaeozoic found in this area. The fourth volume in the Lower Palaeozoic Rocks of the World Series discusses geological formations such as the lower Cambrian tribolite-bearing sequences of Morocco, those showing evidence of late Ordovician glaciation in West-Central Africa, and the Silurian-Devonian sequences of Morocco and Algeria.

  11. Genomic analysis identifies candidate pathogenic variants in 9 of 18 patients with unexplained West syndrome.

    PubMed

    Hino-Fukuyo, Naomi; Kikuchi, Atsuo; Arai-Ichinoi, Natsuko; Niihori, Tetsuya; Sato, Ryo; Suzuki, Tasuku; Kudo, Hiroki; Sato, Yuko; Nakayama, Tojo; Kakisaka, Yosuke; Kubota, Yuki; Kobayashi, Tomoko; Funayama, Ryo; Nakayama, Keiko; Uematsu, Mitsugu; Aoki, Yoko; Haginoya, Kazuhiro; Kure, Shigeo

    2015-06-01

    West syndrome, which is narrowly defined as infantile spasms that occur in clusters and hypsarrhythmia on EEG, is the most common early-onset epileptic encephalopathy (EOEE). Patients with West syndrome may have clear etiologies, including perinatal events, infections, gross chromosomal abnormalities, or cases followed by other EOEEs. However, the genetic etiology of most cases of West syndrome remains unexplained. DNA from 18 patients with unexplained West syndrome was subjected to microarray-based comparative genomic hybridization (array CGH), followed by trio-based whole-exome sequencing in 14 unsolved families. We identified candidate pathogenic variants in 50% of the patients (n = 9/18). The array CGH revealed candidate pathogenic copy number variations in four cases (22%, 4/18), including an Xq28 duplication, a 16p11.2 deletion, a 16p13.1 deletion and a 19p13.2 deletion disrupting CACNA1A. Whole-exome sequencing identified candidate mutations in known epilepsy genes in five cases (36%, 5/14). Three candidate de novo mutations were identified in three cases, with two mutations occurring in two new candidate genes (NR2F1 and CACNA2D1) (21%, 3/14). Hemizygous candidate mutations in ALG13 and BRWD3 were identified in the other two cases (14%, 2/14). Evaluating a panel of 67 known EOEE genes failed to identify significant mutations. Despite the heterogeneity of unexplained West syndrome, the combination of array CGH and whole-exome sequencing is an effective means of evaluating the genetic background in unexplained West syndrome. We provide additional evidence for NR2F1 as a causative gene and for CACNA2D1 and BRWD3 as candidate genes for West syndrome. PMID:25877686

  12. Genomic analysis identifies candidate pathogenic variants in 9 of 18 patients with unexplained West syndrome.

    PubMed

    Hino-Fukuyo, Naomi; Kikuchi, Atsuo; Arai-Ichinoi, Natsuko; Niihori, Tetsuya; Sato, Ryo; Suzuki, Tasuku; Kudo, Hiroki; Sato, Yuko; Nakayama, Tojo; Kakisaka, Yosuke; Kubota, Yuki; Kobayashi, Tomoko; Funayama, Ryo; Nakayama, Keiko; Uematsu, Mitsugu; Aoki, Yoko; Haginoya, Kazuhiro; Kure, Shigeo

    2015-06-01

    West syndrome, which is narrowly defined as infantile spasms that occur in clusters and hypsarrhythmia on EEG, is the most common early-onset epileptic encephalopathy (EOEE). Patients with West syndrome may have clear etiologies, including perinatal events, infections, gross chromosomal abnormalities, or cases followed by other EOEEs. However, the genetic etiology of most cases of West syndrome remains unexplained. DNA from 18 patients with unexplained West syndrome was subjected to microarray-based comparative genomic hybridization (array CGH), followed by trio-based whole-exome sequencing in 14 unsolved families. We identified candidate pathogenic variants in 50% of the patients (n = 9/18). The array CGH revealed candidate pathogenic copy number variations in four cases (22%, 4/18), including an Xq28 duplication, a 16p11.2 deletion, a 16p13.1 deletion and a 19p13.2 deletion disrupting CACNA1A. Whole-exome sequencing identified candidate mutations in known epilepsy genes in five cases (36%, 5/14). Three candidate de novo mutations were identified in three cases, with two mutations occurring in two new candidate genes (NR2F1 and CACNA2D1) (21%, 3/14). Hemizygous candidate mutations in ALG13 and BRWD3 were identified in the other two cases (14%, 2/14). Evaluating a panel of 67 known EOEE genes failed to identify significant mutations. Despite the heterogeneity of unexplained West syndrome, the combination of array CGH and whole-exome sequencing is an effective means of evaluating the genetic background in unexplained West syndrome. We provide additional evidence for NR2F1 as a causative gene and for CACNA2D1 and BRWD3 as candidate genes for West syndrome.

  13. Stratigraphic sequence analysis of the Antler foreland

    SciTech Connect

    Silberling, N.J.; Nichols, K.M.; Macke, D.L. )

    1993-04-01

    Mid-Upper Devonian to Upper Mississippian strata in western Utah were deposited in the distal Antler foreland. They record lateral and vertical changes in depositional environments that define five successive stratigraphic sequences, each representing a third-order transgressive-regressive cycle. In ascending order, these sequences are informally named the Langenheim (LA) of late Frasnian to mid-Famennian age, the Gutschick (GU) of late Famennian to early Kinderhookian age, the Morris (MO) of late Kinderhookian age; the Sadlick (SA) of Osagean to early Meramecian age, and the Maughan (MA) of mid-Meramecian to Chesterian age. MO is widespread and recognized within carbonate rocks of the Fitchville Formation and Joana Limestone. SA formed in concert with and to the east and south of the Wendover foreland high; the Delle phosphatic event marks maximum marine flooding during SA deposition. The transgressive systems tract of MA includes rhythmic-bedded limestone in the upper part of the Deseret Limestone in west-central Utah and, farther west, the hypoxic limestone and black shale of the Skunk Spring Limestone Bed and part of the overlying Chainman Shale. Traced westward into Nevada, MA first oversteps SA and then MO. Lithostratigraphic correlation of these sequences still farther west into the Eureka thrust belt (ETB) could mean that the youngest strata truncated by the Roberts Mountains thrust belong to the MA and that this thrust is simply part of the post-Mississippian ETB. However, some strata in central Nevada that lithically resemble those of the MA are paleontologically dated as Early Mississippian, the age of sequences overstepped by MA not far to the east. Thus, at least some imbricates of the ETB may contain a sequence stratigraphy which reflects local tectonic control.

  14. [Differential diagnoses of West syndrome].

    PubMed

    Fejerman, Natalio

    2013-09-01

    This study describes the clinical and electroencephalographic characteristics of epileptic spasms, and more especially those that occur during the first two years of life (infantile spasms). West syndrome has been clearly defined as the association between infantile spasms with an electroencephalographic pattern of hypsarrhythmia. Although intellectual deficit appears in almost all cases in which infantile spasms are not controlled with medication, this is a developmental aspect of the condition and not a manifestation that must necessarily be present in order to define the syndrome. The analysis of the interictal and ictal electroencephalogram readings, together with the clinical characteristics of the spasms and the neurological examination of patients, provides some orientation as regards the causations. Despite the spectrum that the title of this work focuses on, the study does not cover the treatment of early infants with West syndrome. Emphasis is placed on the differential diagnoses of West syndrome with other epileptic syndromes that manifest in the first two years of life, and more especially with a series of abnormal non-epileptic motor phenomena that occur in early infants. All these last non-epileptic disorders are displayed in a table, but benign myoclonus of early infancy or Fejerman syndrome is given as a paradigmatic example for the differential diagnosis. The primordial aim is to prevent neurologically healthy early infants from receiving antiepileptic drugs and even adrenocorticotropic hormone or corticoids due to a mistaken diagnosis.

  15. [West Nile fever/encephalitis].

    PubMed

    Takasaki, Tomohiko

    2007-12-01

    West Nile virus (WNV), a member of the family Flaviviridae (genus Flavivirus), is a mosquito-borne virus first isolated in 1937 in the West Nile district of Uganda. The disease in humans is characterized by a dengue-like illness with fever, and a more severe form is characterized by central nervous system involvement, including encephalitis, meningitis, and myelitis. WN encephalitis was first reported in the Western Hemisphere in the summer of 1999, there was an outbreak in New York City. Epidemic WNV strains in North America are severely pathogenic, however, attenuated WNV strains were found in Texas and Mexico in 2003. The principal vectors of WNV transmission in North America are Culex. pipiens, Cx. Quinquefasciatus, Cx. restuans, Cx salinarius and Cx talsalis. The number of WN fever case has exceeded 27,000 since 1999 in the United States and 4,600 since 2002 in Canada. The first imported case of West Nile fever in Japan was confirmed in September, 2005. The patient had returned to Japan from the United States and developed symptoms the next day. There is currently no WN vaccine for use in humans. An inactivated WNV vaccine for use in horses has been available since 2001. A DNA vaccine, a chimeric live attenuated vaccine, and a recombinant vaccine have also been licensed for use in horses.

  16. West Nile virus isolated from Virginia opossum (Didelphis virginiana) in Northwest Missouri 2012

    DOE PAGESBeta

    Bosco-Lauth, Angela; Harmon, Jessica; Lash, R. Ryan; Weiss, Sonja; Langevin, Stanley; Savage, Harry; Marvin S. Godsey, Jr.; Burkhalter, Kristen; Root, J. Jeffrey; Gidlewski, Thomas; et al

    2014-12-01

    We describe the isolation of West Nile virus (WNV; Flaviviridae, flavivirus) from blood of a Virginia opossum (Didelphis virginiana) collected in northwestern Missouri, USA in August 2012. Furthermore, sequencing determined that the virus was related to lineage 1a WNV02 strains. We discuss the role of wildlife in WNV disease epidemiology.

  17. An Active Classroom: The Emerging Scholars Program at West Virginia University

    ERIC Educational Resources Information Center

    Deshler, Jessica M.; Miller, David; Pascal, Matthew

    2016-01-01

    In an effort to support the success of minority students and to incorporate inquiry-based learning (IBL) into the calculus sequence of courses at West Virginia University, a modified version of the Emerging Scholars Program (ESP) was implemented in the fall of 2009. Since then, approximately 100 students have taken ESP Calculus I, with many of…

  18. Preserving sequence annotations across reference sequences

    PubMed Central

    2014-01-01

    Background Matching and comparing sequence annotations of different reference sequences is vital to genomics research, yet many annotation formats do not specify the reference sequence types or versions used. This makes the integration of annotations from different sources difficult and error prone. Results As part of our effort to create linked data for interoperable sequence annotations, we present an RDF data model for sequence annotation using the ontological framework established by the OBO Foundry ontologies and the Basic Formal Ontology (BFO). We defined reference sequences as the common domain of integration for sequence annotations, and identified three semantic relationships between sequence annotations. In doing so, we created the Reference Sequence Annotation to compensate for gaps in the SO and in its mapping to BFO, particularly for annotations that refer to versions of consensus reference sequences. Moreover, we present three integration models for sequence annotations using different reference assemblies. Conclusions We demonstrated a working example of a sequence annotation instance, and how this instance can be linked to other annotations on different reference sequences. Sequence annotations in this format are semantically rich and can be integrated easily with different assemblies. We also identify other challenges of modeling reference sequences with the BFO. PMID:25093075

  19. WEST ELEVATION OF USAIR MAINTENANCE HANGAR AT GREATER BUFFALO INTERNATIONAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEST ELEVATION OF USAIR MAINTENANCE HANGAR AT GREATER BUFFALO INTERNATIONAL AIRPORT. A BOEING 737-200 HAS BEEN TOWED IN FOR AN OVERNIGHT (BALANCE) CHECK. THE TAIL DOCK STANDS ARE IN POSITION AT THE REAR OF THE AIRCRAFT TO FACILITATE INSPECTION. MAINTENANCE CREWS PERFORM NIGHTLY SERVICE ON UP TO 6 AIRCRAFT. THE NORMAL SEQUENCE OF 12 ROUTINE CHECKS COVERS SEVEN BASIC AREAS: INTERIOR, EXTERIOR, WINGS, LANDING GEAR, TAIL, AUXILIARY POWER UNIT (APU), AND ENGINES. THE WORK FORCE CONSISTS OF 5 INSPECTORS, 3 LEAD MECHANICS, AND 24 MECHANICS; NIGHTLY SCHEDULES ARE COORDINATED BY A PLANNER. - Greater Buffalo International Airport, Maintenance Hangar, Buffalo, Erie County, NY

  20. West Nile virus in the British Virgin Islands.

    PubMed

    Anthony, S J; Garner, M M; Palminteri, L; Navarrete-Macias, I; Sanchez-Leon, M D; Briese, T; Daszak, P; Lipkin, W I

    2014-06-01

    West Nile virus (WNV) first emerged in the US in 1999 and has since spread across the Americas. Here, we report the continued expansion of WNV to the British Virgin Islands following its emergence in a flock of free-roaming flamingos. Histologic review of a single chick revealed lesions consistent with WNV infection, subsequently confirmed with PCR, immunohistochemistry and in situ hybridization. Full genome analysis revealed 99% sequence homology to strains circulating in the US over the past decade. This study highlights the need for rapid necropsy of wild bird carcasses to fully understand the impact of WNV on wild populations.

  1. West Nile Virus in Mosquitoes of Iranian Wetlands.

    PubMed

    Bagheri, Masoomeh; Terenius, Olle; Oshaghi, Mohammad Ali; Motazakker, Morteza; Asgari, Sassan; Dabiri, Farrokh; Vatandoost, Hassan; Mohammadi Bavani, Mulood; Chavshin, Ali Reza

    2015-12-01

    The West Nile virus (WNV) transmission cycle includes a wide range of migratory wetland birds as reservoirs, mosquitoes as biological vectors, and equines and humans as dead-end hosts. Despite the presence of potential vector species, there is no information about the existence of WNV in mosquito vectors in Iran. The Iranian West Azerbaijan Province is located in the northwestern part of Iran and has borders with Turkey, Iraq, Armenia, and the Republic of Azerbaijan. The current study was conducted to identify the wetland mosquitoes of the West Azerbaijan Province and their infection with WNV. In this study, 2143 specimens were collected, comprising 1541 adults and 602 larvae. Six species belonging to four genera were collected and identified: Anopheles maculipennis sensu lato (s.l.), Culex (Cx.) hortensis, Cx. pipiens s.l., Cx. theileri, Culiseta longiareolata, and Aedes (Ae.) (Ochlerotatus) caspius. In total, 45 pools of mosquitoes were examined. Two of the adult pools collected from the same location showed the presence of WNV in Ae. (Och.) caspius, from Sangar, Makoo County, as confirmed by PCR and sequencing. Due to the discovery of WNV in the mosquito population of the region, and the presence of wetlands and significant populations of migratory birds, the health sector should carefully monitor the factors involved in the cycle of this disease. PMID:26565610

  2. WDR45 mutations in three male patients with West syndrome.

    PubMed

    Nakashima, Mitsuko; Takano, Kyoko; Tsuyusaki, Yu; Yoshitomi, Shinsaku; Shimono, Masayuki; Aoki, Yoshihiro; Kato, Mitsuhiro; Aida, Noriko; Mizuguchi, Takeshi; Miyatake, Satoko; Miyake, Noriko; Osaka, Hitoshi; Saitsu, Hirotomo; Matsumoto, Naomichi

    2016-07-01

    West syndrome is an early-onset epileptic encephalopathy characterized by clustered spasms with hypsarrhythmia seen on electroencephalogram (EEG). West syndrome is genetically heterogeneous, and its genetic causes have not been fully elucidated. WD Repeat Domain 45 (WDR45) resides on Xp11.23, and encodes a member of the WD repeat protein interacting with phosphoinositides (WIPI) family, which is crucial in the macroautophagy pathway. De novo mutations in WDR45 cause beta-propeller protein-associated neurodegeneration characterized by iron accumulation in the basal ganglia. In this study, we performed whole exome sequencing of individuals with West syndrome and identified three WDR45 mutations in three independent males (patients 1, 2 and 3). Two novel mutations occurred de novo (patients 1 and 2) and the remaining mutation detected in a male patient (patient 3) and his affected sister was inherited from the mother, harboring the somatic mutation. The three male patients showed early-onset intractable seizures, profound intellectual disability and developmental delay. Their brain magnetic resonance imaging scans showed cerebral atrophy. We found no evidence of somatic mosaicism in the three male patients. Our findings indicate that hemizygous WDR45 mutations in males lead to severe epileptic encephalopathy.

  3. Persistence of West Nile virus.

    PubMed

    Garcia, Melissa N; Hasbun, Rodrigo; Murray, Kristy O

    2015-02-01

    West Nile virus (WNV) is a widespread global pathogen that results in significant morbidity and mortality. Data from animal models provide evidence of persistent renal and neurological infection from WNV; however, the possibility of persistent infection in humans and long-term neurological and renal outcomes related to viral persistence remain largely unknown. In this paper, we provide a review of the literature related to persistent infection in parallel with the findings from cohorts of patients with a history of WNV infection. The next steps for enhancing our understanding of WNV as a persistent pathogen are discussed.

  4. Shotgun protein sequencing.

    SciTech Connect

    Faulon, Jean-Loup Michel; Heffelfinger, Grant S.

    2009-06-01

    A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins.

  5. 4. VIEW WEST, WEST SIDE, SHOWING CHANNELS 1ST AND 2ND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW WEST, WEST SIDE, SHOWING CHANNELS 1ST AND 2ND VERTICAL BRACED DOUBLE ANGLES, DIAGONAL BRACING AND CROSS BRACED RAILING - Thirty-Sixth Street Bridge, Spanning Rabbit River, Hamilton, Allegan County, MI

  6. The sequence of sequencers: The history of sequencing DNA

    PubMed Central

    Heather, James M.; Chain, Benjamin

    2016-01-01

    Determining the order of nucleic acid residues in biological samples is an integral component of a wide variety of research applications. Over the last fifty years large numbers of researchers have applied themselves to the production of techniques and technologies to facilitate this feat, sequencing DNA and RNA molecules. This time-scale has witnessed tremendous changes, moving from sequencing short oligonucleotides to millions of bases, from struggling towards the deduction of the coding sequence of a single gene to rapid and widely available whole genome sequencing. This article traverses those years, iterating through the different generations of sequencing technology, highlighting some of the key discoveries, researchers, and sequences along the way. PMID:26554401

  7. K West Basin canister survey

    SciTech Connect

    Pitner, A.L.

    1998-08-26

    A survey was conducted of the K West Basin to determine the distribution of canister types that contain the irradiated N Reactor fuel. An underwater camera was used to conduct the survey during June 1998, and the results were recorded on videotape. A full row-by-row survey of the entire basin was performed, with the distinction between aluminum and stainless steel Mark 1 canisters made by the presence or absence of steel rings on the canister trunions (aluminum canisters have the steel rings). The results of the survey are presented in tables and figures. Grid maps of the three bays show the canister lid ID number and the canister type in each location that contained fuel. The following abbreviations are used in the grid maps for canister type designation: IA = Mark 1 aluminum, IS = Mark 1 stainless steel, and 2 = Mark 2 stainless steel. An overall summary of the canister distribution survey is presented in Table 1. The total number of canisters found to contain fuel was 3842, with 20% being Mark 1 Al, 25% being Mark 1 SS, and 55% being Mark 2 SS. The aluminum canisters were predominantly located in the East and West bays of the basin.

  8. Quaternary faults of west Texas

    SciTech Connect

    Collins, E.W.; Raney, J.A. . Bureau of Economic Geology)

    1993-04-01

    North- and northwest-striking intermontane basins and associated normal faults in West Texas and adjacent Chihuahua, Mexico, formed in response to Basin and Range tectonism that began about 24 Ma ago. Data on the precise ages of faulted and unfaulted Quaternary deposits are sparse. However, age estimates made on the basis of field stratigraphic relationships and the degree of calcic soil development have helped determine that many of the faults that bound the basin margins ruptured since the middle Pleistocene and that some faults probably ruptured during the Holocene. Average recurrence intervals between surface ruptures since the middle Pleistocene appear to be relatively long, about 10,000 to 100,000 yr. Maximum throw during single rupture events have been between 1 and 3 m. Historic seismicity in West Texas is low compared to seismicity in many parts of the Basin and Range province. The largest historic earthquake, the 1931 Valentine earthquake in Ryan Flat/Lobo Valley, had a magnitude of 6.4 and no reported surface rupture. The most active Quaternary faults occur within the 120-km-long Hueco Bolson, the 70-km-long Red Light Bolson, and the > 200-km-long Salt Basins/Wild Horse Flat/Lobo Valley/Ryan Flat.

  9. West Nile Virus and wildlife

    USGS Publications Warehouse

    Marra, P.P.; Griffing, S.; Caffrey, C.; Kilpatrick, A.M.; McLean, R.; Brand, C.; Saito, E.; Dupuis, A.P.; Kramer, L.; Novak, R.

    2004-01-01

    West Nile virus (WNV) has spread rapidly across North America, resulting in human deaths and in the deaths of untold numbers of birds, mammals, and reptiles. The virus has reached Central America and the Caribbean and may spread to Hawaii and South America. Although tens of thousands of birds have died, and studies of some bird species show local declines, few regionwide declines can be attributed to WNV. Predicting future impacts of WNV on wildlife, and pinpointing what drives epidemics, will require substantial additional research into host susceptibility, reservoir competency, and linkages between climate, mosquitoes, and disease. Such work will entail a collaborative effort between scientists in governmental research groups, in surveillance and control programs, and in nongovernmental organizations. West Nile virus was not the first, and it will not be the last, exotic disease to be introduced to the New World. Its spread in North America highlights the need to strengthen animal monitoring programs and to integrate them with research on disease ecology.

  10. Monogeneans of West African Cichlid Fish: Evolution and Cophylogenetic Interactions

    PubMed Central

    Mendlová, Monika; Desdevises, Yves; Civáňová, Kristína; Pariselle, Antoine; Šimková, Andrea

    2012-01-01

    The goals of this paper were to investigate phylogenetic and evolutionary patterns of cichlid fish from West Africa and their Cichlidogyrus and Scutogyrus monogenean parasites, to uncover the presence of host-parasite cospeciation and to assess the level of morphological adaptation in parasites. This required the following steps, each one representing specific objectives of this paper: (1) to build phylogenetic trees for Cichlidogyrus and Scutogyrus species based on ribosomal DNA sequences, (2) to investigate phylogenetic relationships within West African cichlid fish based on the analysis of mitochondrial cytochrome b DNA sequences, (3) to investigate host-parasite cophylogenetic history to gain clues on parasite speciation process, and (4) to investigate the link between the morphology of the attachment apparatus and parasite phylogeny. Phylogenetic analyses supported the monophyletic origin of the Cichlidogyrus/Scutogyrus group, and suggested that Cichlidogyrus is polyphyletic and that Scutogyrus is monophyletic. The phylogeny of Cichlidae supported the separation of mouthbrooders and substrate-brooders and is consistent with the hypothesis that the mouthbrooding behavior of Oreochromis and Sarotherodon evolved from substrate-brooding behavior. The mapping of morphological characters of the haptor onto the parasite phylogenetic tree suggests that the attachment organ has evolved from a very simple form to a more complex one. The cophylogenetic analyses indicated a significant fit between trees using distance-based tests, but no significant cospeciation signal using tree-based tests, suggesting the presence of parasite duplications and host switches on related host species. This shed some light on the diversification process of Cichlidogyrus species parasitizing West African cichlids. PMID:22662139

  11. Multimodal sequence learning.

    PubMed

    Kemény, Ferenc; Meier, Beat

    2016-02-01

    While sequence learning research models complex phenomena, previous studies have mostly focused on unimodal sequences. The goal of the current experiment is to put implicit sequence learning into a multimodal context: to test whether it can operate across different modalities. We used the Task Sequence Learning paradigm to test whether sequence learning varies across modalities, and whether participants are able to learn multimodal sequences. Our results show that implicit sequence learning is very similar regardless of the source modality. However, the presence of correlated task and response sequences was required for learning to take place. The experiment provides new evidence for implicit sequence learning of abstract conceptual representations. In general, the results suggest that correlated sequences are necessary for implicit sequence learning to occur. Moreover, they show that elements from different modalities can be automatically integrated into one unitary multimodal sequence.

  12. Whole Genome Sequencing

    MedlinePlus

    ... you want to learn. Search form Search Whole Genome Sequencing You are here Home Testing & Services Testing ... the full story, click here . What is whole genome sequencing? Whole genome sequencing is the mapping out ...

  13. 78 FR 79061 - Noise Exposure Map Notice; Key West International Airport, Key West, FL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-27

    ... Federal Aviation Administration Noise Exposure Map Notice; Key West International Airport, Key West, FL... Key West International Airport under the provisions of the Aviation Safety and Noise Abatement Act and...: This notice announces that the FAA finds that the Noise Exposure Maps submitted for the Key...

  14. Coordinate cytokine regulatory sequences

    DOEpatents

    Frazer, Kelly A.; Rubin, Edward M.; Loots, Gabriela G.

    2005-05-10

    The present invention provides CNS sequences that regulate the cytokine gene expression, expression cassettes and vectors comprising or lacking the CNS sequences, host cells and non-human transgenic animals comprising the CNS sequences or lacking the CNS sequences. The present invention also provides methods for identifying compounds that modulate the functions of CNS sequences as well as methods for diagnosing defects in the CNS sequences of patients.

  15. Science sequence design

    NASA Technical Reports Server (NTRS)

    Koskela, P. E.; Bollman, W. E.; Freeman, J. E.; Helton, M. R.; Reichert, R. J.; Travers, E. S.; Zawacki, S. J.

    1973-01-01

    The activities of the following members of the Navigation Team are recorded: the Science Sequence Design Group, responsible for preparing the final science sequence designs; the Advanced Sequence Planning Group, responsible for sequence planning; and the Science Recommendation Team (SRT) representatives, responsible for conducting the necessary sequence design interfaces with the teams during the mission. The interface task included science support in both advance planning and daily operations. Science sequences designed during the mission are also discussed.

  16. Mitochondrial genome of the bullet tuna Auxis rochei from Indo-West Pacific collection provides novel genetic information about two subspecies.

    PubMed

    Li, Mingming; Guo, Liang; Zhang, Heng; Yang, Sen; Chen, Xinghan; Lin, Haoran; Meng, Zining

    2016-09-01

    Previously morphological studies supported the division of the bullet tuna into the two subspecies, Auxis rochei rochei and A. rochei eudorax. As a cosmopolitan species, A. rochei rochei ranges in the Indo-West Pacific and Atlantic oceans, while A. rochei eudorax inhabits in eastern Pacific region. Here, we used the HiSeq next-generation sequencing technique to determine the mitochondrial genome (mitogenome) of A. rochei from Indo-West Pacific collection, and then compared our data with mitogenomic sequences of the Atlantic and eastern Pacific retrieved from NCBI database. Results showed the mitogenome of A. rochei from three geographic collections shared the same genes and gene order, similar to typical teleosts. Also, we examined a low level of nucleotide diversity among these mitogenomic sequences. Interestingly, nucleotide diversity of intra-subspecies (Atlantic versus Indo-West) was higher than that of inter-subspecies (Atlantic versus eastern Pacific, Indo-West versus eastern Pacific).

  17. West African Folktales [and] Instructor's Manual.

    ERIC Educational Resources Information Center

    Gale, Steven H.

    Traditionally, an important function of folktales in West Africa has been to educate, as the older generation imparts knowledge to younger members of the family, tribe, societal unit, or ethnic group both informally in everyday life and more formally within the context of the bush schools. This anthology of West African folk literature offers more…

  18. Environmental History in the American West.

    ERIC Educational Resources Information Center

    Rothman, Hal K.

    1994-01-01

    Asserts that environmental history is an important component of the history of the American west. Discusses the historiography of the American west in relationship to environmental issues. Concludes that environmental history opened the door for scholars to study the way human beings interact with the physical world around them. (CFR)

  19. Obtaining Financial Parity in West Virginia

    ERIC Educational Resources Information Center

    Bohmann, Joan

    2008-01-01

    On March 28, 2008 West Virginia's governor signed into law House Bill 4117 that provides salary stipends to school psychologists and school nurses who are nationally certified. This accomplishment was largely due to 2 years of work undertaken by three dedicated persons in West Virginia: school psychologists Sarah Kendall and Terry Thorn and school…

  20. An Introduction to West African Literature.

    ERIC Educational Resources Information Center

    Taiwo, Oladele

    Intended to provide help for those interested in studying West African literature, this book is divided into three parts. Part One provides background information: the various African oral traditions are discussed, related to the way of life of the people, and examined for the extent to which they form the basis of present West African literary…

  1. 40 CFR 81.435 - West Virginia.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false West Virginia. 81.435 Section 81.435 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Visibility Is an Important Value § 81.435 West Virginia. Area name Acreage Public Law establishing...

  2. West German Teachers on Today's Issues.

    ERIC Educational Resources Information Center

    Molnar, Alex; And Others

    1984-01-01

    In 1983, elementary and secondary teachers in West Germany were surveyed concerning the treatment of social themes in school curricula. As in a similar survey conducted in the United States, West German respondents indicated that every theme surveyed was important, with nuclear disarmament and environmental pollution clearly considered the most…

  3. 40 CFR 81.435 - West Virginia.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false West Virginia. 81.435 Section 81.435 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Visibility Is an Important Value § 81.435 West Virginia. Area name Acreage Public Law establishing...

  4. 40 CFR 81.435 - West Virginia.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false West Virginia. 81.435 Section 81.435 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Visibility Is an Important Value § 81.435 West Virginia. Area name Acreage Public Law establishing...

  5. 40 CFR 81.435 - West Virginia.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false West Virginia. 81.435 Section 81.435 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Visibility Is an Important Value § 81.435 West Virginia. Area name Acreage Public Law establishing...

  6. 40 CFR 81.435 - West Virginia.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 18 2014-07-01 2014-07-01 false West Virginia. 81.435 Section 81.435 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Visibility Is an Important Value § 81.435 West Virginia. Area name Acreage Public Law establishing...

  7. West Tennessee Research Development Consortium. Final Report.

    ERIC Educational Resources Information Center

    Colmey, James W.

    The West Tennessee Research Development Consortium, formed to increase the potentiality of research in two small West Tennessee Colleges, consists of a project designed to train in research methodology one research person on each of the two campuses, and to offer concurrently an inservice training program to eight faculty members in each of the…

  8. Remote sensing in West Virginia

    NASA Technical Reports Server (NTRS)

    Lessing, P.

    1981-01-01

    Low altitude black and white aerial photography is the prinicipal remote sensing tool for geologic investigations in West Virginia, although side looking radar and color infrared photography are also used. The first land use/cover map for the state was produced in color infrared and is being digitized. Linear features in Cabell and Wayne Counties, as revealed by LANDSAT, were evaluated to test the possible correlations with rock fractures and gas production from shales. A LANDSAT linear features map (1:250,000) was prepared for the entire state, also. Presently investigations are being made to understand karst and to predict areas that should not be used for development. Aerial photography and field mapping is being conducted to detect the location and causes of landslides.

  9. West Maui Groundwater Flow Model

    SciTech Connect

    Nicole Lautze

    2015-01-01

    Groundwater flow model for West Maui. Data is from the following sources: Whittier, R. and A.I. El-Kadi. 2014. Human and Environmental Risk Ranking of Onsite Sewage Disposal Systems For the Hawaiian Islands of Kauai, Molokai, Maui, and Hawaii – Final. Prepared by the University of Hawaii, Dept. of Geology and Geophysics for the State of Hawaii Dept. of Health, Safe Drinking Water Branch. September 2014; and Whittier, R.B., K. Rotzoll, S. Dhal, A.I. El-Kadi, C. Ray, G. Chen, and D. Chang. 2004. Hawaii Source Water Assessment Program Report – Volume V – Island of Maui Source Water Assessment Program Report. Prepared for the Hawaii Department of Health, Safe Drinking Water Branch. University of Hawaii, Water Resources Research Center. Updated 2008.

  10. Consolidated environmental regulation in West Virginia

    SciTech Connect

    Flannery, D.M.; Beckett, K.G.; McThomas, M.P.

    1995-05-01

    In 1994, West Virginia enacted the single largest piece of legislation in its history. The 1,400-page bill that made up this legislation was the crowning achievement of more than a decade of efforts to consolidate and streamline West Virginia`s environmental regulatory programs. The result has been the empowerment of the West Virginia Division of Environmental Protection (DEP) as the centerpiece of environmental regulation in West Virginia. This Article explores the principal initiatives leading to the passage of the legislation empowering the DEP. In addition, it analyzes the substantive provisions of the DEP`s legislative authority and the relationship of that authority to other agencies. Finally, this Article identifies additional areas for the refinement of West Virginia`s environmental regulatory programs.

  11. 41. William E. Barrett, Photographer, 1973. MR. WEST'S HOUSE, MR. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    41. William E. Barrett, Photographer, 1973. MR. WEST'S HOUSE, MR. WEST ON PORCH AND THE ARBOR THAT IS OVER 100 YEARS OLD. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  12. TYPICAL VIEW OF WEST BRANCH COLUMBIA SOUTHERN CANAL OPEN CHANNEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    TYPICAL VIEW OF WEST BRANCH COLUMBIA SOUTHERN CANAL OPEN CHANNEL BETWEEN WEST BRANCH DROP AND GERKING FLUME. LOOKING SOUTH/SOUTHEAST - Tumalo Irrigation District, Tumalo Project, West of Deschutes River, Tumalo, Deschutes County, OR

  13. Interior detail of bathroom on first floor north end, west ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior detail of bathroom on first floor north end, west side, camera facing west. - Mare Island Naval Shipyard, WAVES Officers Quarters, Cedar Avenue, west side between Tisdale Avenue & Eighth Street, Vallejo, Solano County, CA

  14. 14. VIEW LOOKING WEST, GRAIN LEG REMAINS AND CHUTE OPENING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. VIEW LOOKING WEST, GRAIN LEG REMAINS AND CHUTE OPENING OVER RECEIVING HOPPER, ON TRACK DECK - West Shore Railroad, Pier 7 Grain Elevator, Hudson River & Pershing Road vicinity, West New York, Hudson County, NJ

  15. FRONT ELEVATIONS, NOS. 2122 AND 2124 ON THE WEST SIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FRONT ELEVATIONS, NOS. 2122 AND 2124 ON THE WEST SIDE OF UBER STREET, LOOKING WEST. - 2100 Block North Uber Street (Houses), East & west sides between Diamond Street & Susquehanna Avenue, Philadelphia, Philadelphia County, PA

  16. 10. VIEW WEST TOWARD DOWNSTREAM SIDE OF SPILLWAY FROM UNDERSIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. VIEW WEST TOWARD DOWNSTREAM SIDE OF SPILLWAY FROM UNDERSIDE OF GARDEN STATE PARKWAY ABUTMENT - Upper Doughty Dam, 200 feet west of Garden State Parkway, 1.7 miles west of Absecon, Egg Harbor City, Atlantic County, NJ

  17. 9. VIEW WEST TOWARD DOWNSTREAM SIDE OF SPILLWAY FROM NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW WEST TOWARD DOWNSTREAM SIDE OF SPILLWAY FROM NORTH SIDE OF DOWNSTREAM BANK OF DAM - Upper Doughty Dam, 200 feet west of Garden State Parkway, 1.7 miles west of Absecon, Egg Harbor City, Atlantic County, NJ

  18. View west along Tatnic Road and Hartford Road (Route 6) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View west along Tatnic Road and Hartford Road (Route 6) showing West Brooklyn Green, 10 Tatnic Road, and 126 Hartford Road - West Brooklyn Green, Tatnic Road & Hartford Road, Brooklyn, Windham County, CT

  19. 7. Building 7 interior, west end of building showing tier ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Building 7 interior, west end of building showing tier of skylight windows and modern equipment. View looking west. - John & James Dobson Carpet Mill (West Parcel), Building No. 7, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  20. 4. Building 8 interior, west end showing general character of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Building 8 interior, west end showing general character of second floor, with columns and beams. View looking west. - John & James Dobson Carpet Mill (West Parcel), Building No. 8, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  1. View west; interior view of south bay Naval Base ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View west; interior view of south bay - Naval Base Philadelphia-Philadelphia Naval Shipyard, Foundry-Propeller Shop, North of Porter Avenue, west of Third Street West, Philadelphia, Philadelphia County, PA

  2. View west; interior view of central bay Naval Base ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View west; interior view of central bay - Naval Base Philadelphia-Philadelphia Naval Shipyard, Foundry-Propeller Shop, North of Porter Avenue, west of Third Street West, Philadelphia, Philadelphia County, PA

  3. View southeast; west and north facades and context Naval ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View southeast; west and north facades and context - Naval Base Philadelphia-Philadelphia Naval Shipyard, Foundry-Propeller Shop, North of Porter Avenue, west of Third Street West, Philadelphia, Philadelphia County, PA

  4. 6. OBLIQUE VIEW, FROM SOUTHWEST, SHOWING WEST PORTAL, THROUGH TRUSSES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. OBLIQUE VIEW, FROM SOUTHWEST, SHOWING WEST PORTAL, THROUGH TRUSSES OF WEST SPAN, AND PORTION OF WEST APPROACH - Glendale Road Bridge, Spanning Deep Creek Lake on Glendale Road, McHenry, Garrett County, MD

  5. View of intersection with west wall of north wing and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of intersection with west wall of north wing and north wall of west wing; camera facing southeast. - Mare Island Naval Shipyard, Marine Prison, Suisun Avenue, west side between Mesa Road & San Pablo, Vallejo, Solano County, CA

  6. Corridor in west wing Fitzsimons General Hospital, Women's Army ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Corridor in west wing - Fitzsimons General Hospital, Women's Army Corps Recreation & Administration Building, North Hickey Street, west side, 75 feet north of intersection of West Pennington Avenue & North Hickey Street, Aurora, Adams County, CO

  7. North and west sides of building Fitzsimons General Hospital, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North and west sides of building - Fitzsimons General Hospital, Civilian Employees' Quarters, North Hickey Street, West side, 150 feet North of intersection of North Hickey Street & West Loosley Avenue, Aurora, Adams County, CO

  8. East is east and West is west: perspectives on the menopause in Asia and The West.

    PubMed

    Baber, R J

    2014-02-01

    There is a school of thought that believes that menopausal symptoms are a peculiarly 'Western' phenomenon, not experienced by women from other regions and particularly not from Asia where, it has been claimed, dietary, social and cultural factors afforded protection for women living in that region. More recently, studies conducted in multi-ethnic communities living in Western countries as well as in Asian communities have found that the menopause and its consequences are similar world-wide. Ethnic differences within Asia account for small differences in endogenous hormone levels and age at menopause between Asian and Western women, and the type of menopause symptoms and their prevalence also differ between those two communities. However, like in the West and perhaps because of a Western influence, the long-term health problems of postmenopausal women including cardiovascular disease, osteoporosis and breast cancer are of major importance to Asian women and health services in the 21st century.

  9. 5. Historic American Buildings Survey Cervin Robinson, Photographer 1957 WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey Cervin Robinson, Photographer 1957 WEST HALL FROM BALCONY - Massachusetts Charitable Mechanics Association, Exhibition Hall, Huntington Avenue & West Newton Street, Boston, Suffolk County, MA

  10. GENERAL VIEW, WEST SIDE OF UBER STREET, LOOKING SOUTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    GENERAL VIEW, WEST SIDE OF UBER STREET, LOOKING SOUTHWEST. - 2100 Block North Uber Street (Houses), East & west sides between Diamond Street & Susquehanna Avenue, Philadelphia, Philadelphia County, PA

  11. Overview from west bank of Schuykill River. Philadelphia & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Overview from west bank of Schuykill River. - Philadelphia & Reading Railroad, Bridge at West Falls, Spanning Schuylkill River, southeast of Roosevelt Boulevard Bridge, Philadelphia, Philadelphia County, PA

  12. The generalized quaternion sequence

    NASA Astrophysics Data System (ADS)

    Deveci, Ömür

    2016-04-01

    In this work, we define the recurrence sequence by using the relation matrix of the generalized quaternion group and then, we obtain miscellaneous properties of this sequence. Also, we obtain the cyclic groups and the semigroups which are produced by generating matrix of the sequence defined when read modulo m. Furthermore, we study this sequence modulo m, and then we derive the relationship among the order the cyclic groups obtained and the periods of the sequence defined.

  13. A novel HLA-DQB1 allele, DQB1*05022, isolated from the Jing ethnic group in South-west China.

    PubMed

    Fu, Y; Chen, W; Liu, Z; Lin, J; Jia, Z; Pan, D; Xu, A

    2002-07-01

    A novel DQB1 allele, DQB1*05022, has been identified from an individual of the Jing ethnic group in South-west China. The sequence was confirmed by cloning and sequencing. The allele differs from DQB1*05021 at codon 47 (TAC to TAT) and from DQB1*05031 at codon 57 (GAC to AGC).

  14. On determinants of migration in West Bengal.

    PubMed

    Roy, N C

    1987-10-01

    The migration flow to West Bengal from different parts of the Indian subcontinent is an old phenomenon which can be traced to the beginning of the 19th century when the process of industrialization began in the areas comprising the present West Bengal. As West Bengal gradually industrialized and urbanized, the migration flow which was an equiliberating process up to 1947 turned into a stagnating mechanism in the economic sphere. This paper attempts to treat the causative process generating migration flows into West Bengal. Some popular hypotheses are tested, and their inadequacies in the case of migration in West Bengal are shown. Then a variable incorporating the expected long-run socioeconomic condition of West Bengal vis-a-vis the states/countries belonging to the migration universe of West Bengal was constructed, and this expectational variable appears to have more explanatory power than the observed income differential. Barring the political factor which played the central role in the case of immigration from Bangladesh, the determinants can be ranked in order of importance as 1) linguistic distance, 2) expected socioeconomic condition, and 3) physical distance. The limitation of the framework used is the scaling of linguistic distance and the inability to treat the political variable in a causal matrix of migration.

  15. On determinants of migration in West Bengal.

    PubMed

    Roy, N C

    1987-10-01

    The migration flow to West Bengal from different parts of the Indian subcontinent is an old phenomenon which can be traced to the beginning of the 19th century when the process of industrialization began in the areas comprising the present West Bengal. As West Bengal gradually industrialized and urbanized, the migration flow which was an equiliberating process up to 1947 turned into a stagnating mechanism in the economic sphere. This paper attempts to treat the causative process generating migration flows into West Bengal. Some popular hypotheses are tested, and their inadequacies in the case of migration in West Bengal are shown. Then a variable incorporating the expected long-run socioeconomic condition of West Bengal vis-a-vis the states/countries belonging to the migration universe of West Bengal was constructed, and this expectational variable appears to have more explanatory power than the observed income differential. Barring the political factor which played the central role in the case of immigration from Bangladesh, the determinants can be ranked in order of importance as 1) linguistic distance, 2) expected socioeconomic condition, and 3) physical distance. The limitation of the framework used is the scaling of linguistic distance and the inability to treat the political variable in a causal matrix of migration. PMID:12281036

  16. DOLLY SODS WILDERNESS, WEST VIRGINIA.

    USGS Publications Warehouse

    Englund, Kenneth J.; Hill, James J.

    1984-01-01

    Coal, the principal mineral resource of the Dolly Sods Wilderness, West Virginia is in at least seven beds of low- to medium-volatile bituminous rank. Of these beds, four are of sufficient thickness, quality, and extent to contain demonstrated coal resources which are estimated to total about 15. 5 million short tons in areas of substantiated coal resource potential. A Small-scale development of the coal resources of the Dolly Sods Wilderness has been by several shallow adits which provided fuel for locomotives during early logging operations and by a one truck mine. All mine entries are now abandoned. Peat, shale, clay, and sandstone, occur in the area but because of remoteness of markets and inaccessability they are not classified as resources in this report. Natural gas may occur in rocks underlying the area, but because of a lack of subsurface information an estimate of resource potential has not been made. No evidence of metallic-mineral resources was found during this investigation.

  17. Does the geoid drift west?

    NASA Technical Reports Server (NTRS)

    Backus, G. E.; Parker, R. L.; Zumberge, M. A.

    1985-01-01

    In 1970 Hide and Malin noted a correlation of about 0.8 between the geoid and the geomagnetic potential at the Earth's surface when the latter is rotated eastward in longitude by about 160 degrees and the spherical harmonic expansions of both functions are truncated at degree 4. From a century of magnetic observatory data, Hide and Malin inferred an average magnetic westward drift rate of about 0.27 degrees/year. They attributed the magnetic-gravitational correlation to a core event at about 1350 A.D. which impressed the mantle's gravity pattern at long wavelengths onto the core motion and the resulting magnetic field. The impressed pattern was then carried westward 160 degrees by the nsuing magnetic westward drift. An alternative possibility is some sort of steady physical coupling between the magnetic and gravitational fields (perhaps migration of Hide's bumps on the core-mantle interface). This model predicts that the geoid will drift west at the magnetic rate. On a rigid earth, the resulting changes in sea level would be easily observed, but they could be masked by adjustment of the mantle if it has a shell with viscosity considerably less than 10 to the 21 poise. However, steady westward drift of the geoid also predicts secular changes in g, the local acceleration of gravity, at land stations. These changes are now ruled out by recent independent high-accuracy absolute measurements of g made by several workers at various locations in the Northern Hemisphere.

  18. GeoPowering the west.

    SciTech Connect

    Hill, Roger Ray

    2004-09-01

    The U.S. Department of Energy's (DOE's) GeoPowering the West (GPW) program works with the U.S. geothermal industry, power companies, industrial and residential consumers, and federal, state, and local officials to provide technical and institutional support and limited, cost-shared funding to state-level activities. By demonstrating the benefits of geothermal energy, GPW increases state and regional awareness of opportunities to enhance local economies and strengthen our nation's energy security while minimizing environmental impact. By identifying barriers to development and working with others to eliminate them, GPW helps a state or region create a regulatory and economic environment that is more favorable for geothermal and other renewable energy development. Electricity is produced using expanding steam or very hot water from the underground reservoir to spin a conventional turbine-generator. Geothermal power plants operate at high capacity factors (70-100%), with availability factors typically greater than 95%. Geothermal plants are among the cleanest sources of electric power available. Direct use applications directly pipe hot water from geothermal resources to provide heat for industrial processes, crop drying, greenhouses, aquaculture, recreation, sidewalk snow-melting, and buildings. Geothermal district heating systems supply heat to multiple buildings through a network of pipes carrying the hot geothermal water.

  19. West Nile Virus Drug Discovery

    PubMed Central

    Lim, Siew Pheng; Shi, Pei-Yong

    2013-01-01

    The outbreak of West Nile virus (WNV) in 1999 in the USA, and its continued spread throughout the Americas, parts of Europe, the Middle East and Africa, underscored the need for WNV antiviral development. Here, we review the current status of WNV drug discovery. A number of approaches have been used to search for inhibitors of WNV, including viral infection-based screening, enzyme-based screening, structure-based virtual screening, structure-based rationale design, and antibody-based therapy. These efforts have yielded inhibitors of viral or cellular factors that are critical for viral replication. For small molecule inhibitors, no promising preclinical candidate has been developed; most of the inhibitors could not even be advanced to the stage of hit-to-lead optimization due to their poor drug-like properties. However, several inhibitors developed for related members of the family Flaviviridae, such as dengue virus and hepatitis C virus, exhibited cross-inhibition of WNV, suggesting the possibility to re-purpose these antivirals for WNV treatment. Most promisingly, therapeutic antibodies have shown excellent efficacy in mouse model; one of such antibodies has been advanced into clinical trial. The knowledge accumulated during the past fifteen years has provided better rationale for the ongoing WNV and other flavivirus antiviral development. PMID:24300672

  20. Geologic Map of the Denver West 30' x 60' Quadrangle, North-Central Colorado

    USGS Publications Warehouse

    Kellogg, Karl S.; Shroba, Ralph R.; Bryant, Bruce; Premo, Wayne R.

    2008-01-01

    The Denver West quadrangle extends east-west across the entire axis of the Front Range, one of numerous uplifts in the Rocky Mountain region in which Precambrian rocks are exposed. The history of the basement rocks in the Denver West quadrangle is as old as 1,790 Ma. Along the east side of the range, a sequence of sedimentary rocks as old as Pennsylvanian, but dominated by Cretaceous-age rocks, overlies these ancient basement rocks and was upturned and locally faulted during Laramide (Late Cretaceous to early Tertiary) uplift of the range. The increasingly coarser grained sediments up section in rocks of latest Cretaceous to early Tertiary age record in remarkable detail this Laramide period of mountain building. On the west side of the range, a major Laramide fault (Williams Range thrust) places Precambrian rocks over Cretaceous sedimentary rocks. The geologic history of the quadrangle, therefore, can be divided into four major periods: (1) Proterozoic history, (2) Pennsylvanian to pre-Laramide, Late Cretaceous history, (3) Late Cretaceous to early Tertiary Laramide mountain building, and (4) post-Laramide history. In particular, the Quaternary history of the Denver West quadrangle is described in detail, based largely on extensive new mapping.

  1. DPA1*02012: A DPA1*0201-related Mhc class II allele in West Africa

    SciTech Connect

    Meyer, C.G.; May, J.; Spauke, D.; Schnittger, L.

    1994-12-31

    DNA techniques such as sequence-specific oligonucleotide probe (SSOP) hybridizations, restriction-fragment length polymorphism (RFLP) analyses, and DNA sequencing have greatly supported the characterization of Mhc class II allelic polymorphism. Here the authors describe a DPA 1 allele which has been identified in two male individuals from Liberia and Benin, West Africa, during a survey study on Mhc class II associations with the different manifestations after infection with Onchocerca volvulus. 4 refs., 1 fig.

  2. Genome Sequence Databases (Overview): Sequencing and Assembly

    SciTech Connect

    Lapidus, Alla L.

    2009-01-01

    From the date its role in heredity was discovered, DNA has been generating interest among scientists from different fields of knowledge: physicists have studied the three dimensional structure of the DNA molecule, biologists tried to decode the secrets of life hidden within these long molecules, and technologists invent and improve methods of DNA analysis. The analysis of the nucleotide sequence of DNA occupies a special place among the methods developed. Thanks to the variety of sequencing technologies available, the process of decoding the sequence of genomic DNA (or whole genome sequencing) has become robust and inexpensive. Meanwhile the assembly of whole genome sequences remains a challenging task. In addition to the need to assemble millions of DNA fragments of different length (from 35 bp (Solexa) to 800 bp (Sanger)), great interest in analysis of microbial communities (metagenomes) of different complexities raises new problems and pushes some new requirements for sequence assembly tools to the forefront. The genome assembly process can be divided into two steps: draft assembly and assembly improvement (finishing). Despite the fact that automatically performed assembly (or draft assembly) is capable of covering up to 98% of the genome, in most cases, it still contains incorrectly assembled reads. The error rate of the consensus sequence produced at this stage is about 1/2000 bp. A finished genome represents the genome assembly of much higher accuracy (with no gaps or incorrectly assembled areas) and quality ({approx}1 error/10,000 bp), validated through a number of computer and laboratory experiments.

  3. Contamination of sequence databases with adaptor sequences

    SciTech Connect

    Yoshikawa, Takeo; Sanders, A.R.; Detera-Wadleigh, S.D.

    1997-02-01

    Because of the exponential increase in the amount of DNA sequences being added to the public databases on a daily basis, it has become imperative to identify sources of contamination rapidly. Previously, contaminations of sequence databases have been reported to alert the scientific community to the problem. These contaminations can be divided into two categories. The first category comprises host sequences that have been difficult for submitters to manage or control. Examples include anomalous sequences derived from Escherichia coli, which are inserted into the chromosomes (and plasmids) of the bacterial hosts. Insertion sequences are highly mobile and are capable of transposing themselves into plasmids during cloning manipulation. Another example of the first category is the infection with yeast genomic DNA or with bacterial DNA of some commercially available cDNA libraries from Clontech. The second category of database contamination is due to the inadvertent inclusion of nonhost sequences. This category includes incorporation of cloning-vector sequences and multicloning sites in the database submission. M13-derived artifacts have been common, since M13-based vectors have been widely used for subcloning DNA fragments. Recognizing this problem, the National Center for Biotechnology Information (NCBI) started to screen, in April 1994, all sequences directly submitted to GenBank, against a set of vector data retrieved from GenBank by use of key-word searches, such as {open_quotes}vector.{close_quotes} In this report, we present evidence for another sequence artifact that is widespread but that, to our knowledge, has not yet been reported. 11 refs., 1 tab.

  4. Automated DNA Sequencing System

    SciTech Connect

    Armstrong, G.A.; Ekkebus, C.P.; Hauser, L.J.; Kress, R.L.; Mural, R.J.

    1999-04-25

    Oak Ridge National Laboratory (ORNL) is developing a core DNA sequencing facility to support biological research endeavors at ORNL and to conduct basic sequencing automation research. This facility is novel because its development is based on existing standard biology laboratory equipment; thus, the development process is of interest to the many small laboratories trying to use automation to control costs and increase throughput. Before automation, biology Laboratory personnel purified DNA, completed cycle sequencing, and prepared 96-well sample plates with commercially available hardware designed specifically for each step in the process. Following purification and thermal cycling, an automated sequencing machine was used for the sequencing. A technician handled all movement of the 96-well sample plates between machines. To automate the process, ORNL is adding a CRS Robotics A- 465 arm, ABI 377 sequencing machine, automated centrifuge, automated refrigerator, and possibly an automated SpeedVac. The entire system will be integrated with one central controller that will direct each machine and the robot. The goal of this system is to completely automate the sequencing procedure from bacterial cell samples through ready-to-be-sequenced DNA and ultimately to completed sequence. The system will be flexible and will accommodate different chemistries than existing automated sequencing lines. The system will be expanded in the future to include colony picking and/or actual sequencing. This discrete event, DNA sequencing system will demonstrate that smaller sequencing labs can achieve cost-effective the laboratory grow.

  5. Libraries in West Virginia: MedlinePlus

    MedlinePlus

    ... this page: https://medlineplus.gov/libraries/westvirginia.html Libraries in West Virginia To use the sharing features ... enable JavaScript. Bridgeport United Hospital Center Health Sciences Library 327 Medical Park Dr Bridgeport, WV 26330 681- ...

  6. Eighteenth annual West Coast theoretical chemistry conference

    SciTech Connect

    1997-05-01

    Abstracts are presented from the eighteenth annual west coast theoretical chemistry conference. Topics include molecular simulations; quasiclassical simulations of reactions; photodissociation reactions; molecular dynamics;interface studies; electronic structure; and semiclassical methods of reactive systems.

  7. GOES-West Video of Sandra

    NASA Video Gallery

    An animation of visible and infrared imagery from NOAA's GOES-West satellite showed Tropical Storm Sandra has become decoupled by strong wind shear as it was approaching landfall in western Mexico ...

  8. FAQ: General Questions about West Nile Virus

    MedlinePlus

    ... or meningitis (inflammation of the lining of the brain and spinal cord). West Nile virus transmission has been documented in Europe and the Middle East, Africa, India, parts of Asia, and Australia. It was first detected ...

  9. GOES-West Satellite Sees 'Pineapple Express'

    NASA Video Gallery

    Visible and infrared images taken from NOAA's Geostationary Operational Environmental, or GOES-West, satellite from Feb. 7 through 9, 2014, were animated by the NASA GOES Project at NASA's Goddard ...

  10. GOES-West Movie of Hurricane Newton

    NASA Video Gallery

    This animation of infrared and visible images from NOAA's GOES-West satellite shows the development and movement of Hurricane Newton from Sept. 4 through Sept. 6 at 10 a.m. EDT (1400 UTC) toward Ba...

  11. Petrology of the Plutonic Rocks of west-central Alaska

    USGS Publications Warehouse

    Miller, Thomas P.

    1970-01-01

    A series of plutons in west-central Alaska defines the Hogatza plutonic belt which extends for about 200 miles in an east-west direction from the northeastern Seward Peninsula to the Koyukuk River. The plutonic rocks have an aggregate area of about 1,200 square miles and their composition, distribution, and possible petrogenesis are discussed for the first time in this report. Field, petrographic and chemical data supported by K/Ar age dating indicate the plutonic rocks are divisible into two suites differing in age, location, and composition. The western plutons are mid-Cretaceous (~100 m.y.) in age and consist of a heterogeneous assemblage of monzonite, syenite, quartz monzonite. Associated with these granitic rocks is a group of alkaline sub-silicic rocks that forma belt of intrusive complexes extending for a distance of at least 180 miles from west-central Alaska to the Bering Sea. The complex at Granite Mountain shows a rare example of zoning from an alkaline rim to a quartz-bearing core. The occurrence of a similar complex at Cape Dezhnev on the easternmost tip of Siberia suggests the alkaline province may extend into Siberia. The easternmost plutons are Late Cretaceous (180 m.y.) in age and composed primarily of granodiorite and quartz monzonite similar to calc-alkaline plutons found throughout the North America Cordillera. The plutons are epizonal and intrude deformed but unmetamorphosed Lower Cretaceous andesitic volcanics and volcanic graywacke which constitute the highly mobile Yukon-Koyukuk volcanogenic province of west-central Alaska. No older rocks have been found within the confines of this vast tract; the occurrence of a bounding ophiolite sequence has lead to the suggestion that the province was formed by large-scale rifting and is underlain by oceanic crust. The possibility of no juvenile sialic crust over much of the area suggests that the potassium-rich magma now represented by the alkaline rocks originated in the mantle. The distribution of the

  12. West Nile virus isolated from Virginia opossum (Didelphis virginiana) in Northwest Missouri 2012

    SciTech Connect

    Bosco-Lauth, Angela; Harmon, Jessica; Lash, R. Ryan; Weiss, Sonja; Langevin, Stanley; Savage, Harry; Marvin S. Godsey, Jr.; Burkhalter, Kristen; Root, J. Jeffrey; Gidlewski, Thomas; Nicholson, William; Brault, Aaron C.; Komar, Nicholas

    2014-12-01

    We describe the isolation of West Nile virus (WNV; Flaviviridae, flavivirus) from blood of a Virginia opossum (Didelphis virginiana) collected in northwestern Missouri, USA in August 2012. Furthermore, sequencing determined that the virus was related to lineage 1a WNV02 strains. We discuss the role of wildlife in WNV disease epidemiology.

  13. Sequence information signal processor

    DOEpatents

    Peterson, John C.; Chow, Edward T.; Waterman, Michael S.; Hunkapillar, Timothy J.

    1999-01-01

    An electronic circuit is used to compare two sequences, such as genetic sequences, to determine which alignment of the sequences produces the greatest similarity. The circuit includes a linear array of series-connected processors, each of which stores a single element from one of the sequences and compares that element with each successive element in the other sequence. For each comparison, the processor generates a scoring parameter that indicates which segment ending at those two elements produces the greatest degree of similarity between the sequences. The processor uses the scoring parameter to generate a similar scoring parameter for a comparison between the stored element and the next successive element from the other sequence. The processor also delivers the scoring parameter to the next processor in the array for use in generating a similar scoring parameter for another pair of elements. The electronic circuit determines which processor and alignment of the sequences produce the scoring parameter with the highest value.

  14. Cellulases and coding sequences

    DOEpatents

    Li, Xin-Liang; Ljungdahl, Lars G.; Chen, Huizhong

    2001-02-20

    The present invention provides three fungal cellulases, their coding sequences, recombinant DNA molecules comprising the cellulase coding sequences, recombinant host cells and methods for producing same. The present cellulases are from Orpinomyces PC-2.

  15. Cellulases and coding sequences

    DOEpatents

    Li, Xin-Liang; Ljungdahl, Lars G.; Chen, Huizhong

    2001-01-01

    The present invention provides three fungal cellulases, their coding sequences, recombinant DNA molecules comprising the cellulase coding sequences, recombinant host cells and methods for producing same. The present cellulases are from Orpinomyces PC-2.

  16. Roles of repetitive sequences

    SciTech Connect

    Bell, G.I.

    1991-12-31

    The DNA of higher eukaryotes contains many repetitive sequences. The study of repetitive sequences is important, not only because many have important biological function, but also because they provide information on genome organization, evolution and dynamics. In this paper, I will first discuss some generic effects that repetitive sequences will have upon genome dynamics and evolution. In particular, it will be shown that repetitive sequences foster recombination among, and turnover of, the elements of a genome. I will then consider some examples of repetitive sequences, notably minisatellite sequences and telomere sequences as examples of tandem repeats, without and with respectively known function, and Alu sequences as an example of interspersed repeats. Some other examples will also be considered in less detail.

  17. Nonparametric Combinatorial Sequence Models

    NASA Astrophysics Data System (ADS)

    Wauthier, Fabian L.; Jordan, Michael I.; Jojic, Nebojsa

    This work considers biological sequences that exhibit combinatorial structures in their composition: groups of positions of the aligned sequences are "linked" and covary as one unit across sequences. If multiple such groups exist, complex interactions can emerge between them. Sequences of this kind arise frequently in biology but methodologies for analyzing them are still being developed. This paper presents a nonparametric prior on sequences which allows combinatorial structures to emerge and which induces a posterior distribution over factorized sequence representations. We carry out experiments on three sequence datasets which indicate that combinatorial structures are indeed present and that combinatorial sequence models can more succinctly describe them than simpler mixture models. We conclude with an application to MHC binding prediction which highlights the utility of the posterior distribution induced by the prior. By integrating out the posterior our method compares favorably to leading binding predictors.

  18. DNA sequencing conference, 2

    SciTech Connect

    Cook-Deegan, R.M.; Venter, J.C.; Gilbert, W.; Mulligan, J.; Mansfield, B.K.

    1991-06-19

    This conference focused on DNA sequencing, genetic linkage mapping, physical mapping, informatics and bioethics. Several were used to study this sequencing and mapping. This article also discusses computer hardware and software aiding in the mapping of genes.

  19. Geochemical constraints on magma processes in a peralkaline system - The Paisano volcano, west Texas

    NASA Technical Reports Server (NTRS)

    Mcdonough, W. F.; Nelson, D. O.

    1984-01-01

    Petrographic and trace element data from a continuous stratigraphic sequence of flows from the peralkaline Paisano Volcano in west Texas are used to delineate magnetic processes responsible for the chemical evolution of this volcano. It is shown that crystal fractionation of the phenocrystic assemblage explains the main compositional variation, but magma mixing of a less evolved melt composition also affected the chemical evolution of the volcano.

  20. The habitat of petroleum in the Brazilian marginal and west African basins: A biological marker investigation

    SciTech Connect

    Mello, M.R.; Soldan, A.L. ); Maxwell, J.R. ); Figueira, J. )

    1990-05-01

    A geochemical and biological marker investigation of a variety of oils from offshore Brazil and west Africa, ranging in age from Lower Cretaceous to Tertiary, has been done, with the following aims: (1) assessing the depositional environment of source rocks, (2) correlating the reservoired oils, (3) comparing the Brazilian oils with their west African counterparts. The approach was based in stable isotope data; bulk, elemental, and hydrous pyrolysis results; and molecular studies involving quantitative geological marker investigations of alkanes using GC-MS and GC-MS-MS. The results reveal similarities between groups of oils from each side of the Atlantic and suggest an origin from source rocks deposited in five types of depositional environment: lacustrine fresh water, lacustrine saline water, marine evaporitic/carbonate, restricted marine anoxic, and marine deltaic. In west Africa, the Upper Cretaceous marine anoxic succession (Cenomanian-Santonian) appears to be a major oil producer, but in Brazil it is generally immature. The Brazilian offshore oils have arisen mainly from the pre-salt sequence, whereas the African oils show a balance between origins from the pre-salt and marine sequences. The integration of the geochemical and geological data indicate that new frontiers of hydrocarbon exploration in the west African basins must consider the Tertiary reservoirs in the offshore area of Niger Delta, the reservoirs of the rift sequences in the shallow-water areas of south Gabon, Congo, and Cuanza basins, and the reservoirs from the drift sequences (post-salt) in the deep-water areas of Gabon, Congo Cabinda, and Cuanza basins.

  1. Pathogenic Acanthamoeba strains from water sources in Jamaica, West Indies.

    PubMed

    Lorenzo-Morales, J; Lindo, J F; Martinez, E; Calder, D; Figueruelo, E; Valladares, B; Ortega-Rivas, A

    2005-12-01

    In 2004, samples of tap water and of river and sea water associated with human activities were collected in Jamaica, West Indies, and checked for free-living Acanthamoeba. The morphologies of the cysts and trophozoites observed and the results of PCR-based amplifications with a genus-specific primer pair were used to identify the Acanthamoeba isolates. The potential of each isolate as a human pathogen was then evaluated using thermotolerance and osmotolerance assays and two PCR-based assays for Acanthamoeba pathogenesis. Acanthamoeba were identified in 36.1%, 26.4% and 49.6% of the tap-, river- and sea-water samples collected, respectively. Pathogenic potential was shown by 60.0% of the Acanthamoeba strains isolated from tap water, 68.4% of the strains from river water, and 40.4% of the seawater strains. Sequencing of ribosomal DNA revealed the T1, T2, T4, T5, T7, T9 and T11 genotypes. Isolates of the T4 genotype were collected from tap, rain and sea water and, as expected, exhibited the most pathogenic traits; most were osmotolerant, thermotolerant and expressing extracellular serine protease. This is the first study of the occurrence and distribution of Acanthamoeba in water in the West Indies, and the results confirm the presence of potentially pathogenic strains in Jamaica.

  2. West European magnetic confinement fusion research

    SciTech Connect

    McKenney, B.L.; McGrain, M. . Foreign Applied Sciences Assessment Center); Hazeltine, R.D. . Inst. for Fusion Studies); Gentle, K.W. ); Hogan, J.T. ); Porkolab, M. . Dept. of Physics); Sigmar

    1990-01-01

    This report presents a technical assessment and review of the West European program in magnetic confinement fusion by a panel of US scientists and engineers active in fusion research. Findings are based on the scientific and technical literature, on laboratory reports and preprints, and on the personal experiences and collaborations of the panel members. Concerned primarily with developments during the past 10 years, from 1979 to 1989, the report assesses West European fusion research in seven technical areas: tokamak experiments; magnetic confinement technology and engineering; fusion nuclear technology; alternate concepts; theory; fusion computations; and program organization. The main conclusion emerging from the analysis is that West European fusion research has attained a position of leadership in the international fusion program. This distinction reflects in large measure the remarkable achievements of the Joint European Torus (JET). However, West European fusion prominence extends beyond tokamak experimental physics: the program has demonstrated a breadth of skill in fusion science and technology that is not excelled in the international effort. It is expected that the West European primacy in central areas of confinement physics will be maintained or even increased during the early 1990s. The program's maturity and commitment kindle expectations of dramatic West European advances toward the fusion energy goal. For example, achievement of fusion breakeven is expected first in JET, before 1995.

  3. West Nile virus: North American experience

    USGS Publications Warehouse

    Hofmeister, Erik K.

    2011-01-01

    West Nile virus, a mosquito-vectored flavivirus of the Japanese encephalitis serogroup, was first detected in North America following an epizootic in the New York City area in 1999. In the intervening 11 years since the arrival of the virus in North America, it has crossed the contiguous USA, entered the Canadian provinces bordering the USA, and has been reported in the Caribbean islands, Mexico, Central America and, more recently, South America. West Nile virus has been reported in over 300 species of birds in the USA and has caused the deaths of thousands of birds, local population declines of some avian species, the clinical illness and deaths of thousands of domestic horses, and the clinical disease in over 30 000 Americans and the deaths of over 1000. Prior to the emergence of West Nile virus in North America, St. Louis encephalitis virus and Dengue virus were the only other known mosquito-transmitted flaviviruses in North America capable of causing human disease. This review will discuss the North American experience with mosquito-borne flavivirus prior to the arrival of West Nile virus, the entry and spread of West Nile virus in North America, effects on wild bird populations, genetic changes in the virus, and the current state of West Nile virus transmission.

  4. West Nile virus: North American experience.

    PubMed

    Hofmeister, Erik K

    2011-09-01

    West Nile virus, a mosquito-vectored flavivirus of the Japanese encephalitis serogroup, was first detected in North America following an epizootic in the New York City area in 1999. In the intervening 11 years since the arrival of the virus in North America, it has crossed the contiguous USA, entered the Canadian provinces bordering the USA, and has been reported in the Caribbean islands, Mexico, Central America and, more recently, South America. West Nile virus has been reported in over 300 species of birds in the USA and has caused the deaths of thousands of birds, local population declines of some avian species, the clinical illness and deaths of thousands of domestic horses, and the clinical disease in over 30 000 Americans and the deaths of over 1000. Prior to the emergence of West Nile virus in North America, St. Louis encephalitis virus and Dengue virus were the only other known mosquito-transmitted flaviviruses in North America capable of causing human disease. This review will discuss the North American experience with mosquito-borne flavivirus prior to the arrival of West Nile virus, the entry and spread of West Nile virus in North America, effects on wild bird populations, genetic changes in the virus, and the current state of West Nile virus transmission.

  5. Travellers and Cowboys: Myths of the Irish West.

    ERIC Educational Resources Information Center

    Bisplinghoff, Gretchen

    The recent Irish film "Into the West" (1992) explores the myth of the West on two continents. Images from television and movies appear as a visual reference point within "Into the West"; the main characters, two young Irish boys, are fascinated with the American West of cowboys and Indians as depicted in countless Hollywood versions. The image of…

  6. 1. VIEW, LOOKING EAST, OF THE WEST ELEVATION OF BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW, LOOKING EAST, OF THE WEST ELEVATION OF BUILDING 120. BUILDING 120 HOUSES THE PROTECTIVE FORCE PERSONNEL RESPONSIBLE FOR CONTROLLING ENTRANCE TO AND EGRESS FROM THE ROCKY FLATS PLANT WEST ENTRANCE. - Rocky Flats Plant, Guard Facility, South side of west access road, approximately 2,323 feet east of Colorado Highway 93 & West Road, Golden, Jefferson County, CO

  7. Potential seaways across West Antarctica

    NASA Astrophysics Data System (ADS)

    Vaughan, David G.; Barnes, David K. A.; Fretwell, Peter T.; Bingham, Robert G.

    2011-10-01

    The West Antarctic ice sheet (WAIS) has long been considered vulnerable to rapid retreat and today parts are rapidly losing ice. Projection of future change in WAIS is, however, hampered by our poor understanding of past changes, especially during interglacial periods that could be analogs for the future, but which undoubtedly provide an opportunity for testing predictive models. We consider how ice-loss would open seaways across WAIS; these would likely alter Southern Ocean circulation and climate, and would broadly define the de-glacial state, but they may also have left evidence of their existence in the coastal seas they once connected. We show the most likely routes for such seaways, and that a direct seaway between Weddell and Ross seas, which did not pass through the Amundsen Sea sector, is unlikely. Continued ice-loss at present rates would open seaways between Amundsen and Weddell seas (A-W), and Amundsen and Bellingshausen seas (A-B), in around one thousand years. This timescale indicates potential future vulnerability, but also suggests seaways may have opened in recent interglacial periods. We attempt to test this hypothesis using contemporary bryozoan species assemblages around Antarctica, concluding that anomalously high similarity in assemblages in the Weddell and Amundsen seas supports recent migration through A-W. Other authors have suggested opening of seaways last occurred during Marine Isotope Stage 7a (209 ka BP), but we conclude that opening could have occurred in MIS 5e (100 ka BP) when Antarctica was warmer than present and likely contributed to global sea levels higher than today.

  8. Enhanced virome sequencing using targeted sequence capture

    PubMed Central

    Wylie, Todd N.; Wylie, Kristine M.; Herter, Brandi N.; Storch, Gregory A.

    2015-01-01

    Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral populations. It is culture independent, requires no a priori knowledge of the viruses in the sample, and may provide useful genomic information. However, MSS can lack sensitivity and may yield insufficient data for detailed analysis. We have created a targeted sequence capture panel, ViroCap, designed to enrich nucleic acid from DNA and RNA viruses from 34 families that infect vertebrate hosts. A computational approach condensed ∼1 billion bp of viral reference sequence into <200 million bp of unique, representative sequence suitable for targeted sequence capture. We compared the effectiveness of detecting viruses in standard MSS versus MSS following targeted sequence capture. First, we analyzed two sets of samples, one derived from samples submitted to a diagnostic virology laboratory and one derived from samples collected in a study of fever in children. We detected 14 and 18 viruses in the two sets, comprising 19 genera from 10 families, with dramatic enhancement of genome representation following capture enrichment. The median fold-increases in percentage viral reads post-capture were 674 and 296. Median breadth of coverage increased from 2.1% to 83.2% post-capture in the first set and from 2.0% to 75.6% in the second set. Next, we analyzed samples containing a set of diverse anellovirus sequences and demonstrated that ViroCap could be used to detect viral sequences with up to 58% variation from the references used to select capture probes. ViroCap substantially enhances MSS for a comprehensive set of viruses and has utility for research and clinical applications. PMID:26395152

  9. Enhanced virome sequencing using targeted sequence capture.

    PubMed

    Wylie, Todd N; Wylie, Kristine M; Herter, Brandi N; Storch, Gregory A

    2015-12-01

    Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral populations. It is culture independent, requires no a priori knowledge of the viruses in the sample, and may provide useful genomic information. However, MSS can lack sensitivity and may yield insufficient data for detailed analysis. We have created a targeted sequence capture panel, ViroCap, designed to enrich nucleic acid from DNA and RNA viruses from 34 families that infect vertebrate hosts. A computational approach condensed ∼1 billion bp of viral reference sequence into <200 million bp of unique, representative sequence suitable for targeted sequence capture. We compared the effectiveness of detecting viruses in standard MSS versus MSS following targeted sequence capture. First, we analyzed two sets of samples, one derived from samples submitted to a diagnostic virology laboratory and one derived from samples collected in a study of fever in children. We detected 14 and 18 viruses in the two sets, comprising 19 genera from 10 families, with dramatic enhancement of genome representation following capture enrichment. The median fold-increases in percentage viral reads post-capture were 674 and 296. Median breadth of coverage increased from 2.1% to 83.2% post-capture in the first set and from 2.0% to 75.6% in the second set. Next, we analyzed samples containing a set of diverse anellovirus sequences and demonstrated that ViroCap could be used to detect viral sequences with up to 58% variation from the references used to select capture probes. ViroCap substantially enhances MSS for a comprehensive set of viruses and has utility for research and clinical applications.

  10. Use of Testing for West Nile Virus and Other Arboviruses.

    PubMed

    Vanichanan, Jakapat; Salazar, Lucrecia; Wootton, Susan H; Aguilera, Elizabeth; Garcia, Melissa N; Murray, Kristy O; Hasbun, Rodrigo

    2016-09-01

    In the United States, the most commonly diagnosed arboviral disease is West Nile virus (WNV) infection. Diagnosis is made by detecting WNV IgG or viral genomic sequences in serum or cerebrospinal fluid. To determine frequency of this testing in WNV-endemic areas, we examined the proportion of tests ordered for patients with meningitis and encephalitis at 9 hospitals in Houston, Texas, USA. We identified 751 patients (567 adults, 184 children), among whom 390 (52%) experienced illness onset during WNV season (June-October). WNV testing was ordered for 281 (37%) of the 751; results indicated acute infection for 32 (11%). Characteristics associated with WNV testing were acute focal neurologic deficits; older age; magnetic resonance imaging; empirically prescribed antiviral therapy; worse clinical outcomes: and concomitant testing for mycobacterial, fungal, or other viral infections. Testing for WNV is underutilized, and testing of patients with more severe disease raises the possibility of diagnostic bias in epidemiologic studies. PMID:27537988

  11. Petroleum geology and resources of the West Siberian Basin, Russia

    USGS Publications Warehouse

    Ulmishek, Gregory F.

    2003-01-01

    The West Siberian basin is the largest petroleum basin in the world covering an area of about 2.2 million km2. The basin occupies a swampy plain between the Ural Mountains and the Yenisey River. On the north, the basin extends offshore into the southern Kara Sea. On the west, north, and east, the basin is surrounded by the Ural, Yenisey Ridge, and Turukhan-Igarka foldbelts that experienced major deformations during the Hercynian tectonic event and the Novaya Zemlya foldbelt that was deformed in early Cimmerian (Triassic) time. On the south, the folded Caledonian structures of the Central Kazakhstan and Altay-Sayan regions dip northward beneath the basin?s sedimentary cover. The basin is a relatively undeformed Mesozoic sag that overlies the Hercynian accreted terrane and the Early Triassic rift system. The basement is composed of foldbelts that were deformed in Late Carboniferous?Permian time during collision of the Siberian and Kazakhstan continents with the Russian craton. The basement also includes several microcontinental blocks with a relatively undeformed Paleozoic sedimentary sequence. The sedimentary succession of the basin is composed of Middle Triassic through Tertiary clastic rocks. The lower part of this succession is present only in the northern part of the basin; southward, progressively younger strata onlap the basement, so that in the southern areas the basement is overlain by Toarcian and younger rocks. The important stage in tectono-stratigraphic development of the basin was formation of a deep-water sea in Volgian?early Berriasian time. The sea covered more than one million km2 in the central basin area. Highly organic-rich siliceous shales of the Bazhenov Formation were deposited during this time in anoxic conditions on the sea bottom. Rocks of this formation have generated more than 80 percent of West Siberian oil reserves and probably a substantial part of its gas reserves. The deep-water basin was filled by prograding clastic clinoforms

  12. Use of Testing for West Nile Virus and Other Arboviruses

    PubMed Central

    Vanichanan, Jakapat; Salazar, Lucrecia; Wootton, Susan H.; Aguilera, Elizabeth; Garcia, Melissa N.; Murray, Kristy O.

    2016-01-01

    In the United States, the most commonly diagnosed arboviral disease is West Nile virus (WNV) infection. Diagnosis is made by detecting WNV IgG or viral genomic sequences in serum or cerebrospinal fluid. To determine frequency of this testing in WNV-endemic areas, we examined the proportion of tests ordered for patients with meningitis and encephalitis at 9 hospitals in Houston, Texas, USA. We identified 751 patients (567 adults, 184 children), among whom 390 (52%) experienced illness onset during WNV season (June–October). WNV testing was ordered for 281 (37%) of the 751; results indicated acute infection for 32 (11%). Characteristics associated with WNV testing were acute focal neurologic deficits; older age; magnetic resonance imaging; empirically prescribed antiviral therapy; worse clinical outcomes: and concomitant testing for mycobacterial, fungal, or other viral infections. Testing for WNV is underutilized, and testing of patients with more severe disease raises the possibility of diagnostic bias in epidemiologic studies. PMID:27537988

  13. Career Academy Course Sequences.

    ERIC Educational Resources Information Center

    Markham, Thom; Lenz, Robert

    This career academy course sequence guide is designed to give teachers a quick overview of the course sequences of well-known career academy and career pathway programs from across the country. The guide presents a variety of sample course sequences for the following academy themes: (1) arts and communication; (2) business and finance; (3)…

  14. Uncorrectable sequences and telecommand

    NASA Technical Reports Server (NTRS)

    Ekroot, Laura; Mceliece, R.; Dolinar, S.; Swanson, L.

    1993-01-01

    The purpose of a tail sequence for command link transmission units is to fail to decode, so that the command decoder will begin searching for the start of the next unit. A tail sequence used by several missions and recommended for this purpose by the Consultative Committee on Space Data Standards is analyzed. A single channel error can cause the sequence to decode. An alternative sequence requiring at least two channel errors before it can possibly decode is presented. (No sequence requiring more than two channel errors before it can possibly decode exists for this code.)

  15. Low autocorrelation binary sequences

    NASA Astrophysics Data System (ADS)

    Packebusch, Tom; Mertens, Stephan

    2016-04-01

    Binary sequences with minimal autocorrelations have applications in communication engineering, mathematics and computer science. In statistical physics they appear as groundstates of the Bernasconi model. Finding these sequences is a notoriously hard problem, that so far can be solved only by exhaustive search. We review recent algorithms and present a new algorithm that finds optimal sequences of length N in time O(N {1.73}N). We computed all optimal sequences for N≤slant 66 and all optimal skewsymmetric sequences for N≤slant 119.

  16. Regulatory sequence analysis tools.

    PubMed

    van Helden, Jacques

    2003-07-01

    The web resource Regulatory Sequence Analysis Tools (RSAT) (http://rsat.ulb.ac.be/rsat) offers a collection of software tools dedicated to the prediction of regulatory sites in non-coding DNA sequences. These tools include sequence retrieval, pattern discovery, pattern matching, genome-scale pattern matching, feature-map drawing, random sequence generation and other utilities. Alternative formats are supported for the representation of regulatory motifs (strings or position-specific scoring matrices) and several algorithms are proposed for pattern discovery. RSAT currently holds >100 fully sequenced genomes and these data are regularly updated from GenBank.

  17. Misener sandstone - A complex cyclic sequence

    SciTech Connect

    Shelton, J.W.; Fritz, R.D.; Kuykendall, M.; Hooker, E. )

    1989-08-01

    The Misener sandstone is part of two major transgressive/regressive episodes during the Devonian. The Misener is a prolific reservoir in Oklahoma but is one of the most difficult to predict due to its erratic distribution. Depositional environment, a key to understanding Misener distribution and ultimately reservoir geometry, is determined only by understanding the overall geological setting - petrography, unconformities, stratigraphy, paleogeography, and source. Analyses of composition, textures, and sedimentary features in cores and samples combined with detailed correlation and sequence stratigraphy provide a basic framework for determining Misener facies, which indicate deposition in a marine environment. Types of environment range from tidal ridge to estuarine to tidal flat. Many cores show an overall shallowing-upward Misener sequence and change from a terrigenous to a carbonate regime - from phosphatic sands upward to sandy dolomites. This sequence, compared with the regional configuration of the Woodford Shale, suggests that the Woodford developed in two cycles. The Misener section is genetically equivalent to the lower Woodford transgressive/regressive cycle. A paleogeographic model of the Mid-Continent during Misener deposition shows that with the pre-Woodford paleodrainage system, the most likely source for the Misener is from Simpson subcrops around the Ozark dome; the sand was transported and deposited by west-northwest-trending marine currents. A local model for the Misener is the Kremlin area where sand was deposited in erosional lows before carbonate deposition to form a sequence that reflects both shallowing and facies change.

  18. Blooming Seas West of Ireland

    NASA Technical Reports Server (NTRS)

    2007-01-01

    For several weeks in May and early June, daily satellite images of the North Atlantic Ocean west of Ireland have captured partial glimpses of luxuriant blooms of microscopic marine plants between patches of clouds. On June 4, 2007, the skies over the ocean cleared, displaying the sea's spring bloom in brilliant color. A bright blue bloom stretches north from the Mouth of the River Shannon and tapers off like a plume of blue smoke north of Clare Island. (In the large image, a second bloom is visible to the north, wrapping around County Donegal, on the island's northwestern tip.) The image was captured by the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Terra satellite. Cold, nutrient-stocked water often wells up to the surface from the deeper ocean along coastal shelves and at the edges of ocean currents. When it does, it delivers a boost of nutrients that fuel large blooms of single-celled plants collectively known as phytoplankton. The plants are the foundation of the marine food web, and their proliferation in this area of the North Atlantic explains why the waters of western Ireland support myriad fisheries and populations of large mammals like seals, whales, and dolphins. Like plants on land, phytoplankton make their food through photosynthesis, harnessing sunlight for energy using chlorophyll and other light-capturing pigments. The pigments change the way light reflects off the surface water, appearing as colorful swirls of turquoise and green against the darker blue of the ocean. Though individually tiny, collectively these plants play a big role in Earth's carbon and climate cycles; worldwide, they remove about as much carbon dioxide from the atmosphere during photosynthesis as land plants do. Satellites are the only way to map the occurrence of phytoplankton blooms across the global oceans on a regular basis. That kind of information is important not only to scientists who model carbon and climate, but also to biologists and fisheries

  19. Namibia [South-West Africa].

    PubMed

    1983-01-01

    Namibia, a country of 1,051,700 inhabitants of whom 85.6% are blacks of diverse ethnic and linguistic origins, 7.5% are white, and the rest are of mixed ancestry, has been illegally administered by South Africa since 1966, when a League of Nations mandate was revoked by the UN. The Namibian Desert was a barrier to European expansion until the late 18th century, when the area came under German and British influence. Efforts to bring about an orderly and peaceful transition to independent status are hampered at present by the lack of parallel progress toward withdrawal of Cuban combat forces from Angola. Beginning in 1980, considerable executive power was transferred from the administrator general appointed by the South African Government to an interim 3-tier system of elected representatives dividing responsibility between central, ethnic, and local authorities. The judicial structure has separate overlapping systems for whites, westernized blacks and coloreds and for indigenous blacks. Namibian society is highly politicized, with 4 white and about 40 nonwhite political groups. The South West Africa People's Organization (SWAPO) remains an active party inside Namibia despite simultaneous detention of its entire leadership in 1979 by the South African Government. Namibia's economy is dual, with a modern market sector of mining, ranching and fishing producing most of the wealth and a traditional subsistence sector supporting most of the labor force. About 60% of the work force of 500,000 in 1981 worked in agriculture, 19% in industry and commerce, 6% in mining, 8% in services, and 7% in government. Namibia's gross domestic product in 1980 was $1.712 billion, representing an average growth rate of 2.5% from 1970-80. However, real growth since 1978 has been negative because of persistent drought, political uncertainty, low demand for mineral products, and previous overfishing. Namibia has no separate representation in any international body. The country may have the

  20. Stratigraphy of the Grande Savane Ignimbrite Sequence, Dominica, Lesser Antilles

    NASA Astrophysics Data System (ADS)

    Schneider, S.; Smith, A. L.; Deuerling, K.; Killingsworth, N.; Daly, G.

    2007-12-01

    The island of Dominica, located in the central part of the Lesser Antilles island arc has eight potentially active volcanoes. One of these, Morne Diablotins, is a composite stratovolcano with several superimposed stratigraphic sequences ranging in age from Pliocene (4-2 Ma) to "Younger" Pleistocene (<1.8 Ma). The most recent major eruptive activity from this volcano was a series of Plinian eruptions that produced ignimbrites that gave dates of >22,000 and >40,000 years B.P. The ignimbrite sequences form four flow fans that reached both the east and west coasts of the island. One of these flow fans, the Grande Savane, on the west coast of the island, also extends off-shore for a distance of at least 14 km as a distinctive submarine fan. Stratigraphical studies of the on- shore deposits that make up this fan indicate an older sequence of block and ash flow deposits, within which occurs a distinctive vulcanian fall deposit. These are overlain, with no evidence of an intervening paleosol, by a sequence of ignimbrites containing welded horizons (ranging in thickness from around 4 m to 16m). The lack of fall deposits beneath the ignimbrites suggest they may have been formed by instantaneous continuous collapse of the eruption column. This whole succession is overlain by a series of planar and dune bedded pumiceous surge deposits with interbedded pumiceous lapilli fall and ash fall deposits, that extend laterally outside of the main area of ignimbrite deposition. Beds within this upper sequence often contain accretionary lapilli and gas cavities suggesting magma-water interaction. The youngest deposits from Morne Diablotins appear to be valley- fill deposits of both ignimbrite and block and ash flow. A comparison of the of the Grande Savane pyroclastic sequence with the Pointe Ronde (west coast) and Londonderry (east coast) pyroclastic flow fans will provide information on the eruptive history of this major Plinian episode.

  1. 7. West elevation, facing east (Note: B/W scale on west ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. West elevation, facing east (Note: B/W scale on west elevation in 1/2 ft increments) - Nevada Test Site, Reactor Maintenance & Disassembly Complex, Junior Hot Cell, Jackass Flats, Area 25, South of intersection of Roads F & G, Mercury, Nye County, NV

  2. 78 FR 22193 - Special Local Regulations; West Palm Beach Triathlon Championship, Intracoastal Waterway; West...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-15

    ..., Intracoastal Waterway, West Palm Beach, FL'' in the Federal Register (78 FR 2916). We received no comments on...: Table of Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulations; West Palm Beach...

  3. 77 FR 56125 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; Amendments to West...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-12

    ... adopted a PSD program under 45 CSR 14, and the program became part of the West Virginia SIP. See 51 FR..., EPA published a notice of proposed rulemaking (NPR) for West Virginia. 77 FR 10423. The ] NPR proposed... review by the Office of Management and Budget under Executive Order 12866 (58 FR 51735, October 4,...

  4. 33 CFR 110.189a - Key West Harbor, Key West, Fla., naval explosives anchorage area.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...., naval explosives anchorage area. 110.189a Section 110.189a Navigation and Navigable Waters COAST GUARD..., Key West, Fla., naval explosives anchorage area. (a) The anchorage ground. A circular area with its... this section shall be enforced by the Commander, U.S. Naval Base, Key West, Fla., and any...

  5. SOUTH WING, TRA661. WEST SIDE. CAMERA FACING NORTHEAST. MTR WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SOUTH WING, TRA-661. WEST SIDE. CAMERA FACING NORTHEAST. MTR WEST WALL BEYOND ROOF LINE. INL NEGATIVE NO. HD46-45-1. Mike Crane, Photographer, 4/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  6. REACTOR SERVICES BUILDING, TRA635. EAST, WEST, AND SOUTH ELEVATIONS. WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    REACTOR SERVICES BUILDING, TRA-635. EAST, WEST, AND SOUTH ELEVATIONS. WEST ELEVATION HAS TWO STORIES. IDO MTR-635-IDO-7-A, 6/1953. INL INDEX NO. 531-0635-00-396-110590, REV. 1. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  7. PTPN22 polymorphisms may indicate a role for this gene in atopic dermatitis in West Highland white terriers

    PubMed Central

    2011-01-01

    Background Canine atopic dermatitis is an allergic inflammatory skin disease common in West Highland white terriers. A genome-wide association study for atopic dermatitis in a population of West Highland white terriers identified a 1.3 Mb area of association on CFA17 containing canine protein tyrosine phosphatase non-receptor type 22 (lymphoid) PTPN22. This gene is a potential candidate gene for canine atopic dermatitis as it encodes a lymphoid-specific signalling mediator that regulates T-cell and possibly B-cell activity. Findings Sequencing of PTPN22 in three atopic and three non-atopic West Highland white terriers identified 18 polymorphisms, including five genetic variants with a bioinformatically predicted functional effect. An intronic polymorphic repeat sequence variant was excluded as the cause of the genome-wide association study peak signal, by large-scale genotyping in 72 West Highland white terriers (gene-dropping simulation method, P = 0.01). Conclusions This study identified 18 genetic variants in PTPN22 that might be associated with atopic dermatitis in West Highland white terriers. This preliminary data may direct further study on the role of PTPN22 in this disease. Large scale genotyping and complementary genomic and proteomic assays would be required to assess this possibility. PMID:22208456

  8. Indexing Similar DNA Sequences

    NASA Astrophysics Data System (ADS)

    Huang, Songbo; Lam, T. W.; Sung, W. K.; Tam, S. L.; Yiu, S. M.

    To study the genetic variations of a species, one basic operation is to search for occurrences of patterns in a large number of very similar genomic sequences. To build an indexing data structure on the concatenation of all sequences may require a lot of memory. In this paper, we propose a new scheme to index highly similar sequences by taking advantage of the similarity among the sequences. To store r sequences with k common segments, our index requires only O(n + NlogN) bits of memory, where n is the total length of the common segments and N is the total length of the distinct regions in all texts. The total length of all sequences is rn + N, and any scheme to store these sequences requires Ω(n + N) bits. Searching for a pattern P of length m takes O(m + m logN + m log(rk)psc(P) + occlogn), where psc(P) is the number of prefixes of P that appear as a suffix of some common segments and occ is the number of occurrences of P in all sequences. In practice, rk ≤ N, and psc(P) is usually a small constant. We have implemented our solution and evaluated our solution using real DNA sequences. The experiments show that the memory requirement of our solution is much less than that required by BWT built on the concatenation of all sequences. When compared to the other existing solution (RLCSA), we use less memory with faster searching time.

  9. Cenozoic motion between East and West Antarctica

    PubMed

    Cande; Stock; Muller; Ishihara

    2000-03-01

    The West Antarctic rift system is the result of late Mesozoic and Cenozoic extension between East and West Antarctica, and represents one of the largest active continental rift systems on Earth. But the timing and magnitude of the plate motions leading to the development of this rift system remain poorly known, because of a lack of magnetic anomaly and fracture zone constraints on seafloor spreading. Here we report on magnetic data, gravity data and swath bathymetry collected in several areas of the south Tasman Sea and northern Ross Sea. These results enable us to calculate mid-Cenozoic rotation parameters for East and West Antarctica. These rotations show that there was roughly 180 km of separation in the western Ross Sea embayment in Eocene and Oligocene time. This episode of extension provides a tectonic setting for several significant Cenozoic tectonic events in the Ross Sea embayment including the uplift of the Transantarctic Mountains and the deposition of large thicknesses of Oligocene sediments. Inclusion of this East-West Antarctic motion in the plate circuit linking the Australia, Antarctic and Pacific plates removes a puzzling gap between the Lord Howe rise and Campbell plateau found in previous early Tertiary reconstructions of the New Zealand region. Determination of this East-West Antarctic motion also resolves a long standing controversy regarding the contribution of deformation in this region to the global plate circuit linking the Pacific to the rest of the world.

  10. Cenozoic motion between East and West Antarctica

    PubMed

    Cande; Stock; Muller; Ishihara

    2000-03-01

    The West Antarctic rift system is the result of late Mesozoic and Cenozoic extension between East and West Antarctica, and represents one of the largest active continental rift systems on Earth. But the timing and magnitude of the plate motions leading to the development of this rift system remain poorly known, because of a lack of magnetic anomaly and fracture zone constraints on seafloor spreading. Here we report on magnetic data, gravity data and swath bathymetry collected in several areas of the south Tasman Sea and northern Ross Sea. These results enable us to calculate mid-Cenozoic rotation parameters for East and West Antarctica. These rotations show that there was roughly 180 km of separation in the western Ross Sea embayment in Eocene and Oligocene time. This episode of extension provides a tectonic setting for several significant Cenozoic tectonic events in the Ross Sea embayment including the uplift of the Transantarctic Mountains and the deposition of large thicknesses of Oligocene sediments. Inclusion of this East-West Antarctic motion in the plate circuit linking the Australia, Antarctic and Pacific plates removes a puzzling gap between the Lord Howe rise and Campbell plateau found in previous early Tertiary reconstructions of the New Zealand region. Determination of this East-West Antarctic motion also resolves a long standing controversy regarding the contribution of deformation in this region to the global plate circuit linking the Pacific to the rest of the world. PMID:10724159

  11. The amino acid sequence of Staphylococcus aureus penicillinase.

    PubMed Central

    Ambler, R P

    1975-01-01

    The amino acid sequence of the penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) from Staphylococcus aureus strain PC1 was determined. The protein consists of a single polypeptide chain of 257 residues, and the sequence was determined by characterization of tryptic, chymotryptic, peptic and CNBr peptides, with some additional evidence from thermolysin and S. aureus proteinase peptides. A mistake in the preliminary report of the sequence is corrected; residues 113-116 are now thought to be -Lys-Lys-Val-Lys- rather than -Lys-Val-Lys-Lys-. Detailed evidence for the amino acid sequence has been deposited as Supplementary Publication SUP 50056 (91 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1975) 145, 5. PMID:1218078

  12. Intimate Partner Violence among West African Immigrants

    PubMed Central

    AKINSULURE-SMITH, ADEYINKA M.; CHU, TRACY; KEATLEY, EVA; RASMUSSEN, ANDREW

    2013-01-01

    Although the number of African immigrants arriving to the United States has increased significantly, there has been little investigation regarding their experiences of intimate partner violence or coping strategies. This study used focus groups and individual interviews to explore intimate partner violence among 32 heterosexual West African immigrants. Results suggest that although cultural expectations influence their coping strategies, West African–born men and women face different realities, with women reporting multiple instances of abuse and a sense of frustration with the existing options for assistance. Although participants discussed multilevel support structures within the immediate West African community to address intimate partner violence, all of these options maintained a gender hierarchy, leaving women dissatisfied. Challenges and barriers to partner violence resolution and coping strategies are identified. Results are examined in terms of their implications for addressing the needs of this underserved population. Implications for future research and services are discussed and highlighted. PMID:23730146

  13. Impacts of West Nile Virus on wildlife

    USGS Publications Warehouse

    Saito, E.K.; Wild, M.A.

    2004-01-01

    The recent epidemic of West Nile virus in the United States proved to be unexpectedly active and was the largest epidemic of the virus ever recorded. Much remains to be discovered about the ecology and epidemiology of West Nile virus in the United States, including which species are important in maintaining the virus in nature, why some species are more susceptible to lethal infection, and what environmental factors are important in predicting future epidemics. These factors will likely vary regionally, depending on local ecological characteristics. Until scientists better understand the virus and factors influencing its activity, predicting its effects for future seasons is impossible. However, experts are certain about one thing: West Nile virus is here to stay.

  14. West Nile Virus: Review of the Literature

    PubMed Central

    Petersen, Lyle R.; Brault, Aaron C.; Nasci, Roger S.

    2015-01-01

    IMPORTANCE Since its introduction in North America in 1999,West Nile virus has produced the 3 largest arboviral neuroinvasive disease outbreaks ever recorded in the United States. OBJECTIVE To review the ecology, virology, epidemiology, clinical characteristics, diagnosis, prevention, and control of West Nile virus, with an emphasis on North America. EVIDENCE REVIEW PubMed electronic database was searched through February 5, 2013. United States national surveillance data were gathered from the Centers for Disease Control and Prevention. FINDINGS West Nile virus is now endemic throughout the contiguous United States, with 16 196 human neuroinvasive disease cases and 1549 deaths reported since 1999. More than 780 000 illnesses have likely occurred. To date, incidence is highest in the Midwest from mid-July to early September. West Nile fever develops in approximately 25% of those infected, varies greatly in clinical severity, and symptoms may be prolonged. Neuroinvasive disease (meningitis, encephalitis, acute flaccid paralysis) develops in less than 1% but carries a fatality rate of approximately 10%. Encephalitis has a highly variable clinical course but often is associated with considerable long-term morbidity. Approximately two-thirds of those with paralysis remain with significant weakness in affected limbs. Diagnosis usually rests on detection of IgM antibody in serum or cerebrospinal fluid. Treatment is supportive; no licensed human vaccine exists. Prevention uses an integrated pest management approach, which focuses on surveillance, elimination of mosquito breeding sites, and larval and adult mosquito management using pesticides to keep mosquito populations low. During outbreaks or impending outbreaks, emphasis shifts to aggressive adult mosquito control to reduce the abundance of infected, biting mosquitoes. Pesticide exposure and adverse human health events following adult mosquito control operations for West Nile virus appear negligible. CONCLUSIONS AND

  15. Multiyear Persistence of 2 Pandemic A/H1N1 Influenza Virus Lineages in West Africa

    PubMed Central

    Nelson, Martha I.; Njouom, Richard; Viboud, Cecile; Niang, Mbayame N. D.; Kadjo, Hervé; Ampofo, William; Adebayo, Adedeji; Tarnagda, Zekiba; Miller, Mark A.; Holmes, Edward C.; Diop, Ousmane M.

    2014-01-01

    Our understanding of the global ecology of influenza viruses is impeded by historically low levels of viral surveillance in Africa. Increased genetic sequencing of African A/H1N1 pandemic influenza viruses during 2009–2013 revealed multiyear persistence of 2 viral lineages within West Africa, raising questions about the roles of reduced air traffic and the asynchrony of seasonal influenza epidemics among West African countries in the evolution of independent lineages. The potential for novel influenza virus lineages to evolve within Africa warrants intensified influenza surveillance in Africa and other understudied areas. PMID:24446525

  16. Plans for West Coast STS launch capability

    NASA Technical Reports Server (NTRS)

    Murphy, W. P.; Marshall, J. H.

    1979-01-01

    The layout and scope of operations of the Vandenberg AFB West Coast Launch Site for the Space Shuttle are examined. The launch site intended for the first West Coast launch in December 1983 is being constructed by the United States Air Force. Topic discussed include site/selection/management/concepts, Shuttle hardware flow and related facilities, launch pad operations, orbiter erection at SLC-6 VAFB, solid rocket booster operations, and payload ground processing. In conclusion it is noted that NASA and DOD personnel are working together to ensure that the launch site is completed on schedule at minimum cost.

  17. 200 West Area Dust Mitigation Strategies

    SciTech Connect

    Sackschewsky, Michael R.; Becker, James M.

    2001-04-12

    Various strategies were developed for the purpose of mitigating respirable dust experienced at facilities in the southwest corner of the 200 West Area. These strategies focused on treatment of that portion of the dust source located within the 200 West Expansion Area. Strategies included direct shielding of the facilities via establishment of a poplar windbreak and installation of an artificial windscreen; soil stabilization via seeding of herbaceous plants, soil fixatives, straw crimping, straw blankets, gravel mulches, drift fences, baled straw, and living fences; and various irrigation systems that would function both to water seeded herbs and to suppress dust.

  18. Ilheus Virus Isolation in the Pantanal, West-Central Brazil

    PubMed Central

    Pauvolid-Corrêa, Alex; Kenney, Joan L.; Couto-Lima, Dinair; Campos, Zilca M. S.; Nogueira, Rita M. R.; Brault, Aaron C.; Komar, Nicholas

    2013-01-01

    The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal. PMID:23875051

  19. Functional Analysis of West Nile Virus Proteins in Human Cells.

    PubMed

    Kaufusi, Pakieli H; Tseng, Alanna; Nerurkar, Vivek R

    2016-01-01

    West Nile Virus (WNV) lineage 2 strains have been responsible for large outbreaks of neuroinvasive disease in the United States and Europe between 1999 and 2012. Different strains in this lineage have previously been shown to produce either severe or mild neuroinvasive disease in mice. Phylogenetic and amino acid comparisons between highly or less virulent lineage 2 strains have demonstrated that the nonstructural (NS) gene(s) were most variable. However, the roles of some of the NS proteins in virus life cycle are unknown. The aim of this chapter is to describe simple computational and experimental approaches that can be used to: (1) explore the possible roles of the NS proteins in virus life cycle and (2) test whether the subtle amino acid changes in WNV NS gene products contributed to the evolution of more virulent strains. The computational approaches include methods based on: (1) sequence similarity, (2) sequence motifs, and (3) protein membrane topology predictions. Highlighted experimental procedures include: (1) isolation of viral RNA from WNV-infected cells, (2) cDNA synthesis and PCR amplification of WNV genes, (3) cloning into GFP expression vector, (4) bacterial transformation, (5) plasmid isolation and purification, (6) transfection using activated dendrimers (Polyfect), and (7) immunofluorescence staining of transfected mammalian cells. PMID:27188549

  20. Sequences for Student Investigation

    ERIC Educational Resources Information Center

    Barton, Jeffrey; Feil, David; Lartigue, David; Mullins, Bernadette

    2004-01-01

    We describe two classes of sequences that give rise to accessible problems for undergraduate research. These problems may be understood with virtually no prerequisites and are well suited for computer-aided investigation. The first sequence is a variation of one introduced by Stephen Wolfram in connection with his study of cellular automata. The…

  1. Agriculture: Scope and Sequence.

    ERIC Educational Resources Information Center

    Nashville - Davidson County Metropolitan Public Schools, TN.

    This guide, which was written as an initial step in the development of a systemwide articulated curriculum sequence for all vocational programs within the Metropolitan Nashville Public School System, outlines the suggested scope and sequence of a 3-year program in agriculture. The guide consists of a course description; general course objectives;…

  2. M&m Sequences

    ERIC Educational Resources Information Center

    Schultz, Harris S.; Shiflett, Ray C.

    2005-01-01

    Consider a sequence recursively formed as follows: Start with three real numbers, and then when k are known, let the (k +1)st be such that the mean of all k +1 equals the median of the first k. The authors conjecture that every such sequence eventually becomes stable. This article presents results related to their conjecture.

  3. Cosmetology: Scope and Sequence.

    ERIC Educational Resources Information Center

    Nashville - Davidson County Metropolitan Public Schools, TN.

    This scope and sequence guide, developed for a cosmetology vocational education program, represents an initial step in the development of a systemwide articulated curriculum sequence for all vocational programs within the Metropolitan Nashville Public School System. It was developed as a result of needs expressed by teachers, parents, and the…

  4. Lichenase and coding sequences

    DOEpatents

    Li, Xin-Liang; Ljungdahl, Lars G.; Chen, Huizhong

    2000-08-15

    The present invention provides a fungal lichenase, i.e., an endo-1,3-1,4-.beta.-D-glucanohydrolase, its coding sequence, recombinant DNA molecules comprising the lichenase coding sequences, recombinant host cells and methods for producing same. The present lichenase is from Orpinomyces PC-2.

  5. Twin Mitochondrial Sequence Analysis.

    PubMed

    Bouhlal, Yosr; Martinez, Selena; Gong, Henry; Dumas, Kevin; Shieh, Joseph T C

    2013-09-01

    When applying genome-wide sequencing technologies to disease investigation, it is increasingly important to resolve sequence variation in regions of the genome that may have homologous sequences. The human mitochondrial genome challenges interpretation given the potential for heteroplasmy, somatic variation, and homologous nuclear mitochondrial sequences (numts). Identical twins share the same mitochondrial DNA (mtDNA) from early life, but whether the mitochondrial sequence remains similar is unclear. We compared an adult monozygotic twin pair using high throughput-sequencing and evaluated variants with primer extension and mitochondrial pre-enrichment. Thirty-seven variants were shared between the twin individuals, and the variants were verified on the original genomic DNA. These studies support highly identical genetic sequence in this case. Certain low-level variant calls were of high quality and homology to the mitochondrial DNA, and they were further evaluated. When we assessed calls in pre-enriched mitochondrial DNA templates, we found that these may represent numts, which can be differentiated from mtDNA variation. We conclude that twin identity extends to mitochondrial DNA, and it is critical to differentiate between numts and mtDNA in genome sequencing, particularly since significant heteroplasmy could influence genome interpretation. Further studies on mtDNA and numts will aid in understanding how variation occurs and persists. PMID:24040623

  6. West Nile virus circulation in South-Eastern Romania, 2011 to 2013.

    PubMed

    Dinu, S; Cotar, A I; Pănculescu-Gătej, I R; Fălcuţă, E; Prioteasa, F L; Sîrbu, A; Oprişan, G; Bădescu, D; Reiter, P; Ceianu, C S

    2015-05-21

    Lineage 2 West Nile virus (WNV), previously found only in sub-Saharan Africa and Madagascar, was identified in Hungary in 2004 and has rapidly expanded in Europe in the past decade. Following a significant outbreak of West Nile fever with neurological cases caused by lineage 1 WNV in Romania in 1996, scattered cases have been recorded in the south-east of the country in each transmission season. Another outbreak, affecting a larger area and caused by lineage 2 WNV, was recorded in 2010. We analysed human sera from neuroinvasive West Nile fever cases and mosquitoes, sampled in south-eastern Romania between 2011 and 2013, for the presence of WNV genome, and obtained partial NS5 and envelope glycoprotein sequences. Human- and mosquito-derived WNV sequences were highly similar (99%) to Volgograd 2007 lineage 2 WNV and differed from isolates previously detected in central and southern Europe. WNV was detected in one pool of Culex pipiens s.l. males, documenting vertical transmission. Lineage 4 WNV, of unknown pathogenicity to mammals, was found in the amphibian-feeding mosquito Uranotaenia unguiculata from the Danube Delta. Our results present molecular evidence for the maintenance of the same isolates of Volgograd 2007-like lineage 2 WNV in south-eastern Romania between 2011 and 2013.

  7. Biological and phylogenetic characteristics of yellow fever virus lineages from West Africa.

    PubMed

    Stock, Nina K; Laraway, Hewád; Faye, Ousmane; Diallo, Mawlouth; Niedrig, Matthias; Sall, Amadou A

    2013-03-01

    The yellow fever virus (YFV), the first proven human-pathogenic virus, although isolated in 1927, is still a major public health problem, especially in West Africa where it causes outbreaks every year. Nevertheless, little is known about its genetic diversity and evolutionary dynamics, mainly due to a limited number of genomic sequences from wild virus isolates. In this study, we analyzed the phylogenetic relationships of 24 full-length genomes from YFV strains isolated between 1973 and 2005 in a sylvatic context of West Africa, including 14 isolates that had previously not been sequenced. By this, we confirmed genetic variability within one genotype by the identification of various YF lineages circulating in West Africa. Further analyses of the biological properties of these lineages revealed differential growth behavior in human liver and insect cells, correlating with the source of isolation and suggesting host adaptation. For one lineage, repeatedly isolated in a context of vertical transmission, specific characteristics in the growth behavior and unique mutations of the viral genome were observed and deserve further investigation to gain insight into mechanisms involved in YFV emergence and maintenance in nature.

  8. Sequence History Update Tool

    NASA Technical Reports Server (NTRS)

    Khanampompan, Teerapat; Gladden, Roy; Fisher, Forest; DelGuercio, Chris

    2008-01-01

    The Sequence History Update Tool performs Web-based sequence statistics archiving for Mars Reconnaissance Orbiter (MRO). Using a single UNIX command, the software takes advantage of sequencing conventions to automatically extract the needed statistics from multiple files. This information is then used to populate a PHP database, which is then seamlessly formatted into a dynamic Web page. This tool replaces a previous tedious and error-prone process of manually editing HTML code to construct a Web-based table. Because the tool manages all of the statistics gathering and file delivery to and from multiple data sources spread across multiple servers, there is also a considerable time and effort savings. With the use of The Sequence History Update Tool what previously took minutes is now done in less than 30 seconds, and now provides a more accurate archival record of the sequence commanding for MRO.

  9. Genome sequence analysis of Ebola virus in clinical samples from three British healthcare workers, August 2014 to March 2015.

    PubMed

    Bell, A; Lewandowski, K; Myers, R; Wooldridge, D; Aarons, E; Simpson, A; Vipond, R; Jacobs, M; Gharbia, S; Zambon, M

    2015-01-01

    We determined complete viral genome sequences from three British healthcare workers infected with Ebola virus (EBOV) in Sierra Leone, directly from clinical samples. These sequences closely resemble those previously observed in the current Ebola virus disease outbreak in West Africa, with glycoprotein and polymerase genes showing the most sequence variation. Our data indicate that current PCR diagnostic assays remain suitable for detection of EBOV in this epidemic and provide confidence for their continued use in diagnosis.

  10. Writing the New West: A Critical Review

    ERIC Educational Resources Information Center

    Robbins, Paul; Meehan, Katharine; Gosnell, Hannah; Gilbertz, Susan J.

    2009-01-01

    A vast and growing interdisciplinary research effort has focused on the rise of the so-called New West, purportedly the product of regional socioeconomic, political, and ecological upheavals in states like Montana and Colorado. Reviewing the growing research on this problem in sociology, economics, geography, and conservation science, this article…

  11. The Massachusetts TECH PREP WEST Model.

    ERIC Educational Resources Information Center

    1995

    The Tech Prep West Project, established in 1991, brought together three Massachusetts community colleges, seven secondary vocational schools, and one comprehensive high school to develop an alternative college preparation pathway for students, leading from the junior year of high school to an associate degree and employment in a specific career…

  12. Model Program: West Springfield High School, Virginia

    ERIC Educational Resources Information Center

    Alukonis, J. T.; Settar, Scott

    2008-01-01

    West Springfield High School sits on the outskirts of Washington, D.C., in Fairfax County, Virginia. WSHS is home to a thriving and growing technology education program. In recent years, the program has exploded from less than 175 students to well over 425. These numbers are expected to continue to grow in the foreseeable future. In 2002, the…

  13. Problem of hepatocellular carcinoma in West Africa

    PubMed Central

    Ladep, Nimzing G; Lesi, Olufunmilayo A; Mark, Pantong; Lemoine, Maud; Onyekwere, Charles; Afihene, Mary; Crossey, Mary ME; Taylor-Robinson, Simon D

    2014-01-01

    The incidence of hepatocellular carcinoma (HCC) is known to be high in West Africa with an approximate yearly mortality rate of 200000. Several factors are responsible for this. Early acquisition of risk factors; with vertical or horizontal transmission of hepatitis B (HBV), environmental food contaminants (aflatoxins), poor management of predisposing risk factors and poorly-managed strategies for health delivery. There has been a low uptake of childhood immunisation for hepatitis B in many West African countries. Owing to late presentations, most sufferers of HCC die within weeks of their diagnosis. Highlighted reasons for the specific disease pattern of HCC in West Africa include: (1) high rate of risk factors; (2) failure to identify at risk populations; (3) lack of effective treatment; and (4) scarce resources for timely diagnosis. This is contrasted to the developed world, which generally has sufficient resources to detect cases early for curative treatment. Provision of palliative care for HCC patients is limited by availability and affordability of potent analgesics. Regional efforts, as well as collaborative networking activities hold promise that could change the epidemiology of HCC in West Africa. PMID:25429316

  14. West Tennessee ACEI 2006 Fall Conference

    ERIC Educational Resources Information Center

    Clifford, Anna; Hailey, Beth

    2007-01-01

    This article presents the West Tennessee ACEI 2006 Fall Conference held at Union University in Jackson, Tennessee on October 14, 2006. The conference theme, Turning the Pages: A Focus on Children's Literature, was emphasized throughout the day. During the conference, the early childhood classroom teachers, preservice teachers, and administrators…

  15. Protective Behavior and West Nile Virus Risk

    PubMed Central

    Elliott, Susan J.; Gibson, Brian; Fearon, Margaret; Nosal, Robert; Drebot, Michael; D'Cuhna, Colin; Harrington, Daniel; Smith, Stephanie; George, Pauline; Eyles, John

    2005-01-01

    We conducted a cross-sectional, household survey in Oakville, Ontario, where an outbreak of West Nile virus (WNV) in 2002 led to an unprecedented number of cases of meningitis and encephalitis. Practicing >2 personal protective behavior traits reduced the risk for WNV infection by half. PMID:16229774

  16. Transcending Communication Barriers with West Africa.

    ERIC Educational Resources Information Center

    Ampadu, Lena

    Americans doing business with West Africans are limited in their ability to communicate successfully in that part of the world because of language, stereotyping, and ethnocentrism. Americans must become accustomed to British patterns of speech and writing. Stereotypes of Africa, its people, and its cultures perpetuated by the media keep Americans…

  17. Reclamation of abandoned mines in West Virginia

    SciTech Connect

    Dove, J.L.

    1983-01-01

    Reclamation of abandoned mine lands in West Virginia involves disturbed areas from both surface and deep mining activities. Reclamation of deep mine lands deal with mine waste piles and mine openings. Reclamation of surface mine lands involves shaping and grading material to obtain a stable slope and installing water management practices.

  18. Learning to Be: From East and West

    ERIC Educational Resources Information Center

    Jarvis, Peter

    2009-01-01

    The argument of this article is that whilst humanity has evolved for millions of years as a single species, different groups have developed their own cultural differences as they live in different parts of the world, and we learn within our cultures. Research has demonstrated that we do learn differently, in East and West, since we are brought up…

  19. East-West Perspectives on Elder Learning

    ERIC Educational Resources Information Center

    Tam, Maureen

    2012-01-01

    This paper describes and conceptualizes the meaning of lifelong learning from two cultural perspectives--East and West. It examines the different principles underpinning lifelong learning that explain why and how elders in the two cultures engage differently in continued learning. Finally, it discusses the cultural impact on elder learning by…

  20. Retooling Teacher Preparation in West Virginia

    ERIC Educational Resources Information Center

    Manchin, Gayle

    2015-01-01

    This article addresses West Virginia's public schools, and their long struggle with student achievement levels in reading and math. Levels are significantly below the national average and there are poverty-based achievement gaps within the state. In 2013, a cross section of educators and education policy leaders from a range of experiences,…

  1. 77 FR 40793 - West Virginia Regulatory Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-11

    ... in the January 21, 1981, Federal Register (46 FR 5915). You can also find later actions concerning..., Telephone: (304) 347-7158. Email: chfo@osmre.gov . West Virginia Department of Environmental Protection...: Mr. Roger W. Calhoun, Director, Charleston Field Office, Telephone: (304) 347-7158. Email:...

  2. Children Ask Questions about West African Art.

    ERIC Educational Resources Information Center

    Abercrombie, Denice; Cochran, Mathilda; Mims, Margaret

    1997-01-01

    Presents a collection of questions that fifth-grade students asked about African artwork and answers provided by staff from the Museum of Fine Arts in Houston, Texas. Observes that students' interest in important visual aspects of the art creates lead-ins to more detailed discussions of West African art and culture. (DSK)

  3. Go West, Young Man (and Woman)!

    ERIC Educational Resources Information Center

    Byerly, Greg; Brodie, Carolyn S.

    1998-01-01

    Provides an annotated bibliography of Web sites on the American West, the Oregon Trail, Santa Fe Trail, Gold Rush, Donner Party, mountain men and the fur trade, Native Americans, cowboys, and western folklore. Lists related books, activity books, and CD-ROMs on cowboys, migration, and settling. (PEN)

  4. Water supply and needs for West Texas

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This presentation focused on the water supplies and needs of West Texas, Texas High Plains. Groundwater is the most commonly used water resources on the Texas High Plains, with withdrawals from the Ogallala Aquifer dominating. The saturation thickness of the Ogallala Aquifer in Texas is such that t...

  5. Open Syllable Lengthening in West Germanic.

    ERIC Educational Resources Information Center

    Lahiri, Aditi; Dresher, B. Elan

    1999-01-01

    Attempts to show that open syllable lengthening (OSL) was part of the grammar of the West Germanic languages: Middle English, Middle Dutch, and Middle High German. Claims that all three languages endeavored to maintain and maximize the Germanic foot, and OSL contributed in different ways to do so. (Author/VWL)

  6. 76 FR 37996 - West Virginia Regulatory Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-29

    ... Virginia is amending its Code of State Regulations (CSR) to provide for the establishment of a minimum... other things, the establishment of a minimum incremental bonding rate of $10,000 per increment at CSR 38... of approval of the West Virginia program in the January 21, 1981, Federal Register (46 FR 5915)....

  7. 21 CFR 808.98 - West Virginia.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false West Virginia. 808.98 Section 808.98 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES EXEMPTIONS FROM FEDERAL PREEMPTION OF STATE AND LOCAL MEDICAL DEVICE REQUIREMENTS Listing of Specific...

  8. 21 CFR 808.98 - West Virginia.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false West Virginia. 808.98 Section 808.98 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES EXEMPTIONS FROM FEDERAL PREEMPTION OF STATE AND LOCAL MEDICAL DEVICE REQUIREMENTS Listing of Specific...

  9. 21 CFR 808.98 - West Virginia.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false West Virginia. 808.98 Section 808.98 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES EXEMPTIONS FROM FEDERAL PREEMPTION OF STATE AND LOCAL MEDICAL DEVICE REQUIREMENTS Listing of Specific...

  10. 21 CFR 808.98 - West Virginia.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false West Virginia. 808.98 Section 808.98 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES EXEMPTIONS FROM FEDERAL PREEMPTION OF STATE AND LOCAL MEDICAL DEVICE REQUIREMENTS Listing of Specific...

  11. Learning Under Siege on the West Bank

    ERIC Educational Resources Information Center

    Miller, Judith

    1975-01-01

    Birzeit College, the only secular institution of higher education in the Israeli-occupied territories, continues to operate with 500 Palestinians attending classes on the West Bank of the Jordan River Valley even though its president, expelled for alleged subversive activities, continues to run the college from Beirut. (JT)

  12. The West Indian Americans. The New Americans.

    ERIC Educational Resources Information Center

    Henke, Holger

    This book, which is part of a series on new immigrant groups in the United States, captures the experiences of West Indian Americans who have arrived in the country since 1965. The seven chapters include: (1) "History of Jamaica and the English-Speaking Caribbean" (e.g., from plantation society to the third world and the Creolization of cultures…

  13. Natural Hazards within the West Indies.

    ERIC Educational Resources Information Center

    Cross, John A.

    1992-01-01

    Outlines the vulnerability of the West Indies to various natural hazards, especially hurricanes, earthquakes, and volcanic eruptions. Reviews the geologic and meteorologic causes and consequences of the hazards. Suggests methods of incorporating hazards information in geography classes. Includes maps and a hurricane tracking chart. (DK)

  14. 40 CFR 81.349 - West Virginia.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Remainder of State X West Virginia—Carbon Monoxide Designated Area Designation Date 1 Type Classification.... Huntington-Ashland, WV-KY-OH: Cabell County Nonattainment. Mason County (part) Nonattainment. Graham Tax District Wayne County Nonattainment. Marion County, WV (aka Fairmont CBSA): Harrison County (part)...

  15. 40 CFR 81.349 - West Virginia.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Remainder of State X West Virginia—Carbon Monoxide Designated Area Designation Date 1 Type Classification... Attainment. Graham Tax District Wayne County 12/28/12 Attainment. Marion County, WV (aka Fairmont CBSA): Harrison County (part) Tax District of Clay Unclassifiable/Attainment. Marion County...

  16. 40 CFR 81.349 - West Virginia.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... bounded by the Cross Creek Tax District Marshall, WV 1 Marshall County (part) 10-4-13 Nonattainment. Area consisting of Clay Tax district, Franklin Tax District, and Washington Tax District 1 Excludes Indian country located in each area, if any, unless otherwise specified. West Virginia—Carbon Monoxide Designated...

  17. West Virginia Higher Education Report Card, 1998.

    ERIC Educational Resources Information Center

    West Virginia State Coll. and University Systems, Charleston.

    This report card provides information to parents, students, faculty, staff, state policymakers, and the general public as to the quality and performance of public higher education in West Virginia. The report responds to six statutory goals that emerged from the Higher Education Advocacy Team meetings in 1992: (1) better preparing students to…

  18. The West, the Environment, and American History.

    ERIC Educational Resources Information Center

    Harvey, Mark W. T.

    1988-01-01

    Emphasizes that to understand the American West, one must realize the central importance of the environment to its inhabitants. Provides an overview of western environmental history. Discusses state and federal reclamation efforts, the role of public lands, and the growth of conservation and preservation movements. (KO)

  19. Booker T. Washington: Rural West Virginia Teacher.

    ERIC Educational Resources Information Center

    King, Althea M.

    1985-01-01

    The biographical sketch focuses on the early teaching career of Booker T. Washington at Tinkersville School in his hometown of Malden, West Virginia, from 1876-1878. Recollections of Washington's own education in Sunday school, the Tinkersville School, and Hampton Institute are included. (NEC)

  20. West Virginia U. Violated Procedures, Panel Says

    ERIC Educational Resources Information Center

    Fain, Paul

    2008-01-01

    Administrators at West Virginia University violated procedures and displayed poor judgment in their "seriously flawed" response to an inquiry about a high-profile academic transcript, according to a report issued last week by an independent panel. The harshly worded report found that when the "Pittsburgh Post-Gazette" raised questions last October…

  1. Communication Network Design: West Ottawa School District.

    ERIC Educational Resources Information Center

    Couch, David deS.

    This report describes the technical details and rationale behind the decisions in the design and development of the communications network installed as part of a 1991-1993 district-wide construction project in the West Ottawa Public Schools (Michigan). The project called for development of a communications network to carry voice, data, and video…

  2. An Interdisciplinary Project and the American West.

    ERIC Educational Resources Information Center

    Beardsley, Donna A.

    This project began with a list of the kinds of topics that would be of most interest in a study of the old west. Some of the topics that were mentioned for the project included Lewis and Clark, Native American tribes and nations, early pioneers, the fur trade, Texas independence, the Pony Express, homesteaders, gunfighters, the Indian wars, and…

  3. Compensatory Education Policy and the Intermountain West.

    ERIC Educational Resources Information Center

    Murphy, Michael J.; And Others

    Concerned with the impact of Title I, as national education policy on four mountain west states (Utah, Idaho, Wyoming, and Montana), this study concludes that Title I has not been internalized, integrated, or institutionalized in this region of the country or in any of the four individual states. The rejection of Title I policy rests on two basic…

  4. The West Virginia Health Education Assessment Project

    ERIC Educational Resources Information Center

    Tompkins, Nancy O'Hara; Kamal, Khalid M.; Chapman, Don

    2005-01-01

    Well-designed school health education should provide students with the knowledge and skills to prevent the health risk behaviors most responsible for the major causes of morbidity and mortality. This paper reports the methodology and findings of a West Virginia statewide health education assessment initiative and describes how the findings are…

  5. Vaccines in development against West Nile virus.

    PubMed

    Brandler, Samantha; Tangy, Frederic

    2013-10-01

    West Nile encephalitis emerged in 1999 in the United States, then rapidly spread through the North American continent causing severe disease in human and horses. Since then, outbreaks appeared in Europe, and in 2012, the United States experienced a new severe outbreak reporting a total of 5,387 cases of West Nile virus (WNV) disease in humans, including 243 deaths. So far, no human vaccine is available to control new WNV outbreaks and to avoid worldwide spreading. In this review, we discuss the state-of-the-art of West Nile vaccine development and the potential of a novel safe and effective approach based on recombinant live attenuated measles virus (MV) vaccine. MV vaccine is a live attenuated negative-stranded RNA virus proven as one of the safest, most stable and effective human vaccines. We previously described a vector derived from the Schwarz MV vaccine strain that stably expresses antigens from emerging arboviruses, such as dengue, West Nile or chikungunya viruses, and is strongly immunogenic in animal models, even in the presence of MV pre-existing immunity. A single administration of a recombinant MV vaccine expressing the secreted form of WNV envelope glycoprotein elicited protective immunity in mice and non-human primates as early as two weeks after immunization, indicating its potential as a human vaccine.

  6. WEST-3 wind turbine simulator development

    NASA Technical Reports Server (NTRS)

    Hoffman, J. A.; Sridhar, S.

    1985-01-01

    The software developed for WEST-3, a new, all digital, and fully programmable wind turbine simulator is given. The process of wind turbine simulation on WEST-3 is described in detail. The major steps are, the processing of the mathematical models, the preparation of the constant data, and the use of system software generated executable code for running on WEST-3. The mechanics of reformulation, normalization, and scaling of the mathematical models is discussed in detail, in particulr, the significance of reformulation which leads to accurate simulations. Descriptions for the preprocessor computer programs which are used to prepare the constant data needed in the simulation are given. These programs, in addition to scaling and normalizing all the constants, relieve the user from having to generate a large number of constants used in the simulation. Also given are brief descriptions of the components of the WEST-3 system software: Translator, Assembler, Linker, and Loader. Also included are: details of the aeroelastic rotor analysis, which is the center of a wind turbine simulation model, analysis of the gimbal subsystem; and listings of the variables, constants, and equations used in the simulation.

  7. 27 CFR 9.172 - West Elks.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Quadrangle (Colorado-Delta Co. 1955 (photorevised 1978)); (2) Hotchkiss Quadrangle (Colorado-Delta Co. 1965 (photorevised 1979)); (3) Paonia Quadrangle (Colorado-Delta Co. 1965 (photorevised 1979); and (4) Bowie Quadrangle (Colorado-Delta Co. 1965 (photorevised 1978). (c) Boundaries. The West Elks viticultural area...

  8. West Florida shelf upwelling: Origins and pathways

    NASA Astrophysics Data System (ADS)

    Weisberg, Robert H.; Zheng, Lianyuan; Liu, Yonggang

    2016-08-01

    Often described as oligotrophic, the west Florida continental shelf supports abundant fisheries, experiences blooms of the harmful alga, Karenia brevis, and exhibits subsurface chlorophyll maxima evident in shipboard and glider surveys. Renewal of inorganic nutrients by the upwelling of deeper ocean water onto the shelf may account for this, but what are the origins and pathways by which such new water may broach the shelf break and advance toward the shoreline? We address these questions via numerical model simulations of pseudo-Lagrangian, isopycnic water parcel trajectories. Focus is on 2010, when the west Florida shelf was subjected to an anomalously protracted period of upwelling caused by Gulf of Mexico Loop Current interactions with the shelf slope. Origins and pathways are determined by integrating trajectories over successive 45 day intervals, beginning from different locations along the shelf break and at various locations and depths along the shelf slope. Waters upwelling across the shelf break are found to originate from relatively shallow depths along the shelf slope. Even for the anomalous 2010 year, much of this upwelling occurs from about 150 m and above, although waters may broach the shelf break from 300 m depth, particularly in the Florida Panhandle. Such interannual renewal of west Florida shelf waters appears to have profound effects on west Florida shelf ecology.

  9. Missing Chapters: West Virginia Women in History.

    ERIC Educational Resources Information Center

    West Virginia Women's Commission, Charleston.

    Nine women whose lives have contributed to West Virginia history are profiled in these collected essays. These women have made significant contributions to history as: midwife, physician, journalist, photographer, educator, musician, civic activist, and social reformer. The stereotypical image of a powerless, barefooted, uneducated girl is proven…

  10. Neogene molluscan stages of the West Coast of North America

    USGS Publications Warehouse

    Marincovich, L.

    1984-01-01

    Neogene marine sediments of the West Coast of North America were deposited in a series of widely spaced basins that extended geographically from the western and northern Gulf of Alaska (60??N) to southern California (33??N). Rich molluscan faunas occur extensively throughout these deposits and form the basis for biostratigraphic schemes that are useful for correlating within and between individual basins. Early biostratigraphic work was concerned with faunas from particular horizons and with the stratigraphic range of diverse taxa, such as Pecten and Turritella, without reference to other fossil groups. Succeeding work increasingly dealt with the relationships of molluscan zones to benthic and, later, planktonic foraminiferal stages. In recent years the age limits of Neogene molluscan stages have become better documented by reference to planktonic microfossils from dated DSDP cores and onshore faunas. Neogene molluscan faunas from California, the Pacific Northwest states (Oregon and Washington), and southern Alaska have been treated separately due to differences in faunal composition and geographic isolation. As a result, a different biostratigraphic sequence has been described for each region. Pacific Northwest stages have been formally named and defined, and their names are also used informally for Alaskan faunas. California Neogene stages were proposed early in this century, are in need of redescription, and their usage is informal. Precise correlations between the three regional sequences have not yet been achieved, due to the low number of co-occurring species and the general lack of planktonic microfossils in these largely shallow-water faunas. The objectives of ongoing research include: fuller documentation of the faunas of California and Pacific Northwest stages; formal description of California stages; improved correlation between regional stage sequences; refinement of age estimates for stage boundaries; and, establishment of Neogene stages for Alaskan

  11. 40. William E. Barrett, Photographer, 1973. SHOP, YPOLE, MR. WEST'S ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    40. William E. Barrett, Photographer, 1973. SHOP, Y-POLE, MR. WEST'S HOUSE AND POLE SWELL - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  12. 4. VIEW TO EAST SHOWING WEST END OF CRANEWAY (CENTER), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW TO EAST SHOWING WEST END OF CRANEWAY (CENTER), WEST SIDE OF ASSEMBLY BUILDING (LEFT), AND WHARF (RIGHT). - Rosie the Riveter National Historical Park, Ford Assembly Plant, 1400 Harbour Way South, Richmond, Contra Costa County, CA

  13. 8. VIEW OF WEST ELEVATION OF WASTEGATE AND BRIDGE, LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. VIEW OF WEST ELEVATION OF WASTEGATE AND BRIDGE, LOOKING SOUTHEAST, IMMEDIATELY DOWNSTREAM FROM THE SNAKE RIVER VALLEY IRRIGATION DISTRICT - Woodville Canal Company, West side of Snake River (River Mile 796), Woodville, Bingham County, ID

  14. 15. LOOKING WEST TOWARD OLD FAITHFUL INN. THE INN'S NAMESAKE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. LOOKING WEST TOWARD OLD FAITHFUL INN. THE INN'S NAMESAKE, OLD FAITHFUL GEYSER IS AT LEFT. - Old Faithful Inn, 900' northeast of Snowlodge & 1050' west of Old Faithful Lodge, Lake, Teton County, WY

  15. 1. WEST AND SOUTH SIDES OF BUILDING 451. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. WEST AND SOUTH SIDES OF BUILDING 451. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Production Filling & Storage Building, 1050 feet South of December Seventh Avenue; 980 feet West of D Street, Commerce City, Adams County, CO

  16. VIEW OF BUILDING 234 (ARCADE), NORTH WING FROM THE WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF BUILDING 234 (ARCADE), NORTH WING FROM THE WEST END, FACING SOUTHEAST, LOOKING DOWN ARCADE AND ACROSS COURTYARD - Roosevelt Base, Arcade, West Virginia Street, Long Beach, Los Angeles County, CA

  17. Contextual view looking down clubhouse drive. Showing west elevation of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view looking down clubhouse drive. Showing west elevation of H1 on right; camera facing east. - Mare Island Naval Shipyard, Hospital Headquarters, Johnson Lane, west side at intersection of Johnson Lane & Cossey Street, Vallejo, Solano County, CA

  18. 14. UPPER STATION, FRONT AND WEST FACADES, LOOKING NORTHEAST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. UPPER STATION, FRONT AND WEST FACADES, LOOKING NORTHEAST. - Monongahela Incline Plane, Connecting North side of Grandview Avenue at Wyoming Street with West Carson Street near Smithfield Street, Pittsburgh, Allegheny County, PA

  19. 16. UPPER STATION, WEST FACADE, LOOKING EAST SOUTHEAST. Monongahela ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. UPPER STATION, WEST FACADE, LOOKING EAST SOUTHEAST. - Monongahela Incline Plane, Connecting North side of Grandview Avenue at Wyoming Street with West Carson Street near Smithfield Street, Pittsburgh, Allegheny County, PA

  20. 1. VIEW WEST SOUTHWEST, UPPER STATION. INCLINE PLANE TRACK AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW WEST SOUTHWEST, UPPER STATION. INCLINE PLANE TRACK AND LOWER STATION. - Monongahela Incline Plane, Connecting North side of Grandview Avenue at Wyoming Street with West Carson Street near Smithfield Street, Pittsburgh, Allegheny County, PA

  1. 17. UPPER STATION, FIRST FLOOR, WAITING ROOM, LOOKING WEST, NORTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. UPPER STATION, FIRST FLOOR, WAITING ROOM, LOOKING WEST, NORTHWEST. - Monongahela Incline Plane, Connecting North side of Grandview Avenue at Wyoming Street with West Carson Street near Smithfield Street, Pittsburgh, Allegheny County, PA

  2. 17. Rear (west) side of incinerator. Incinerator control panel on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Rear (west) side of incinerator. Incinerator control panel on the right. Looking south towards scrubber cell. - Plutonium Finishing Plant, Waste Incinerator Facility, 200 West Area, Richland, Benton County, WA

  3. 16. Rear (west) side of incinerator. Glove boxes to the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Rear (west) side of incinerator. Glove boxes to the left. Metal catwalk in the middle. Incinerator control panel to the right. Looking south towards scrubber cell. - Plutonium Finishing Plant, Waste Incinerator Facility, 200 West Area, Richland, Benton County, WA

  4. INTERIOR VIEW OF WEST BANK RAILROAD CONTROL TOWER SHOWING INTERLOCKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR VIEW OF WEST BANK RAILROAD CONTROL TOWER SHOWING INTERLOCKING SWITCHING LEVER CONTROL PANEL. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  5. West Lawn Site and Planting Plans Oklahoma City Civic ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West Lawn Site and Planting Plans - Oklahoma City Civic Center, Bounded by N. Shartel Avenue to the West, N. Hudson Avenue to the East, Couch Drive to the North, and Colcord Drive to the South, Oklahoma City, Oklahoma County, OK

  6. 1. WEST AND SOUTH SIDES, FROM APPROXIMATELY 75 FEET SOUTHWEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. WEST AND SOUTH SIDES, FROM APPROXIMATELY 75 FEET SOUTHWEST OF BUILDING, LOOKING EAST-NORTHEAST. - Oakland Naval Supply Center, Heating Plant, North of B Street & West of Third Street, Oakland, Alameda County, CA

  7. 2. WEST AND SOUTH SIDES OF BUILDING 365. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. WEST AND SOUTH SIDES OF BUILDING 365. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Explosives Blending Building, 2180 feet South of December Seventh Avenue; 900 feet West of D Street, Commerce City, Adams County, CO

  8. 21. View to westnorthwest west in electrical room, first floor, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View to west-northwest west in electrical room, first floor, showing AC-DC converters and control panel. - U.S. Navy Fleet Supply Base, Storehouse No. 1, 830 Third Avenue, Brooklyn, Kings County, NY

  9. 4. VIEW OF ENTRANCE TO BUILDING NO. 1 FACING WEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW OF ENTRANCE TO BUILDING NO. 1 FACING WEST. - U.S. Naval Base, Pearl Harbor, Ordinance Operations Building, West Loch, First Street near Whiskey Wharves W1 & W2, Pearl City, Honolulu County, HI

  10. 11. BUILDING 1: FIRST FLOOR (Center Section), WEST AND NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. BUILDING 1: FIRST FLOOR (Center Section), WEST AND NORTH WALLS, SHOWING TWO TIERS OF COLUMNS WITH SECOND FLOOR REMOVED - Boston Beer Company, 225-249 West Second Street, South Boston, Suffolk County, MA

  11. Detail of windows at north portion of west elevation; camera ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of windows at north portion of west elevation; camera facing east. - Mare Island Naval Shipyard, WAVES Officers Quarters, Cedar Avenue, west side between Tisdale Avenue & Eighth Street, Vallejo, Solano County, CA

  12. Interior view of typical room on second floor, west side; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of typical room on second floor, west side; camera facing north. - Mare Island Naval Shipyard, WAVES Officers Quarters, Cedar Avenue, west side between Tisdale Avenue & Eighth Street, Vallejo, Solano County, CA

  13. Detail of entry and stairway at center of west elevation; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of entry and stairway at center of west elevation; camera facing east. - Mare Island Naval Shipyard, WAVES Officers Quarters, Cedar Avenue, west side between Tisdale Avenue & Eighth Street, Vallejo, Solano County, CA

  14. Detail of balcony and windows on west elevation; camera facing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of balcony and windows on west elevation; camera facing northeast. - Mare Island Naval Shipyard, WAVES Officers Quarters, Cedar Avenue, west side between Tisdale Avenue & Eighth Street, Vallejo, Solano County, CA

  15. Detail of windows at center of west elevation; camera facing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of windows at center of west elevation; camera facing east. - Mare Island Naval Shipyard, WAVES Officers Quarters, Cedar Avenue, west side between Tisdale Avenue & Eighth Street, Vallejo, Solano County, CA

  16. Elevation of information window on west wall of south lobby ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Elevation of information window on west wall of south lobby - National Home for Disabled Volunteer Soldiers, Pacific Branch, Main Mental Health Building, 11301 Wilshire Boulevard, West Los Angeles, Los Angeles County, CA

  17. 3. VIEW WEST OF HEADRACE UNDER WAREHOUSE; SLUICE GATES AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW WEST OF HEADRACE UNDER WAREHOUSE; SLUICE GATES AT LEFT CENTER - Willimantic Linen Company, Mill No. 1, Immediately West of South Main Street, North Bank of Willimantic River, Windham, Windham County, CT

  18. 2. VIEW OF WEST AND SOUTH FACADES WITH SCALE STICK, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF WEST AND SOUTH FACADES WITH SCALE STICK, SHOWING BOILER HOUSE IN BACKGROUND, LOOKING NORTHEAST - Marvine Colliery, Heavy Rail Scales Office, West side Boulevard Avenue, between East Parker Street & Route 380, Scranton, Lackawanna County, PA

  19. 18. WEST PART OF SOUTH SIDE OF SOUTH WING AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. WEST PART OF SOUTH SIDE OF SOUTH WING AND PART OF REAR OF FRONT (WEST) PORTION OF BUILDING, LOOKING NORTHWEST (Harms) - Dairy Industry Building, Iowa State University campus, Ames, Story County, IA

  20. 1. NORTH AND WEST SIDES OF BUILDING 1703. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. NORTH AND WEST SIDES OF BUILDING 1703. VIEW TO SOUTHEAST. - Rocky Mountain Arsenal, Ammunition Demolition Building, 3250 feet South of Ninth Avenue; 1750 feet West of E Street, Commerce City, Adams County, CO

  1. 3. VIEW EAST, DETAIL WEST FRONT OF HYDROELECTRIC POWERHOUSE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW EAST, DETAIL WEST FRONT OF HYDROELECTRIC POWERHOUSE - Dayville Mills Hydroelectric Facility, Powerhouse, North side of Route 101, .5 mile west of Route 395, Killingly Center, Windham County, CT

  2. 1. WEST FRONT OF HYDROELECTRIC POWERHOUSE WITH INTAKE STRUCTURE, CANAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. WEST FRONT OF HYDROELECTRIC POWERHOUSE WITH INTAKE STRUCTURE, CANAL SPILLWAY AT LEFT CENTER, VIEW EAST - Dayville Mills Hydroelectric Facility, Powerhouse, North side of Route 101, .5 mile west of Route 395, Killingly Center, Windham County, CT

  3. 3. VIEW SOUTHEAST, WEST END OF DAM AT LEFT CENTER, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW SOUTHEAST, WEST END OF DAM AT LEFT CENTER, HEADGATE STRUCTURE AT CENTER - Dayville Mills Hydroelectric Facility, Dam, North side of Route 101, .5 mile west of Route 395, Killingly Center, Windham County, CT

  4. 2. VIEW EAST, WEST END OF DAM AT CENTER, HEADGATE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW EAST, WEST END OF DAM AT CENTER, HEADGATE OPERATING MECHANISMS AT LEFT - Dayville Mills Hydroelectric Facility, Dam, North side of Route 101, .5 mile west of Route 395, Killingly Center, Windham County, CT

  5. 16. VIEW OF THE HOUSE INTERIOR, WEST AND NORTH WALLS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. VIEW OF THE HOUSE INTERIOR, WEST AND NORTH WALLS OF THE BASEMENT SHOWING FOUNDATION MATERIALS; LOOKING NORTHWEST. - Richard M. Fairbourn Farm, 170 West, 11,400 South, South Jordan, Salt Lake County, UT

  6. House, east side, looking west (bunkhouse in background left) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    House, east side, looking west (bunkhouse in background left) - John Moulton Homestead, House, Approximately 70 feet west of Mormon Row Road, and 300 feet north of Antelope Flats Road, Kelly, Teton County, WY

  7. 1. General view to west, showing context in berth, at ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. General view to west, showing context in berth, at high tide. - Charlestown Navy Yard, Marine Railway, Between Piers 2 & 3, on Charlestown Waterfront at west end of Navy Yard, Boston, Suffolk County, MA

  8. 5. GENERAL VIEW OF GARAGE AREA FROM WEST; STEPS TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. GENERAL VIEW OF GARAGE AREA FROM WEST; STEPS TO WEST FRONT ENTRANCE VISABLE TO THE RIGHT - Isaac N. Hagan House, Kentuck Knob, U.S. Route 40 vicinity (Stewart Township), Chalkhill, Fayette County, PA

  9. 1. West side of bridge. Overall contextual view (looking) eastsoutheast ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. West side of bridge. Overall contextual view (looking) east-southeast or upstream. (Deer Creek flows east to west). 90mm lens. - Gault Bridge, Spanning Deer Creek at South Pine Street, Nevada City, Nevada County, CA

  10. AERIAL VIEW, LOOKING SOUTH WEST, BIRMINGPORT ROAD AND DON DRENNEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    AERIAL VIEW, LOOKING SOUTH WEST, BIRMINGPORT ROAD AND DON DRENNEN OVERPASS IN FOREGROUND, TCI-US STEEL ENSLEY WORKS OPEN HEARTH (RUINS) IN THE BACKGROUND. - Tennessee Coal & Iron Company, Ensley Works, West of residential & commercial districts, Birmingham, Jefferson County, AL

  11. 17. DETAIL OF FRAGMENTS FROM WEST GATE (off site) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. DETAIL OF FRAGMENTS FROM WEST GATE (off site) - Bald Eagle Cross-Cut Canal Lock, North of Water Street along West Branch of Susquehanna River South bank, 500 feet East of Jay Street Bridge, Lock Haven, Clinton County, PA

  12. South Fork Latrine showing north and west sides, general view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South Fork Latrine showing north and west sides, general view to southeast - Fort McKinley, South Fork Latrine, West side of East Side Drive, approximately 225 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  13. Battery Weymouth Combined Observation Station ruin, collapsed; view to west ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Battery Weymouth Combined Observation Station ruin, collapsed; view to west - Fort McKinley, Battery Weymouth Combined Observation Station, West side of East Side Drive, approximately 125 feet south of Weymouth Way, Great Diamond Island, Portland, Cumberland County, ME

  14. 2. EASTSIDE RESERVOIR UNDER CONSTRUCTION LOOKING WEST WITH EAST DAM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. EASTSIDE RESERVOIR UNDER CONSTRUCTION LOOKING WEST WITH EAST DAM IN MIDDLE GROUND, WEST DAM IN DISTANCE. - Eastside Reservoir, Diamond & Domenigoni Valleys, southwest of Hemet, Hemet, Riverside County, CA

  15. 7. INTERIOR VIEW, PATTERN MAKING WORK BENCH AREA WEST WALL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. INTERIOR VIEW, PATTERN MAKING WORK BENCH AREA WEST WALL LOOKING WEST LATHE ON LEFT DELTA BAND SAW LEFT, DELTA 6' JOINTS AND DELTA TABLE SAW ON RIGHT. - Knight Foundry, 13 Eureka Street, Sutter Creek, Amador County, CA

  16. 1. GENERAL VIEW TOWARD NORTHEAST, WEST AND SOUTH FACADES East ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. GENERAL VIEW TOWARD NORTHEAST, WEST AND SOUTH FACADES East facade immediately adjoined by steep hill. North facade obscured by conifers. - U.S. Military Academy, Ice House, Mills Road at Howze Place, West Point, Orange County, NY

  17. 7. DETAIL OF OPEN DOOR, WEST FACADE, SECOND BAY Showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. DETAIL OF OPEN DOOR, WEST FACADE, SECOND BAY Showing door construction and cladding. Head and side jambs splayed. - U.S. Military Academy, Ice House, Mills Road at Howze Place, West Point, Orange County, NY

  18. 2. VIEW TOWARD EAST, WEST FACADE Subsequent views identified by ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW TOWARD EAST, WEST FACADE Subsequent views identified by bay. Four bays numbered sequentially one to four from north. - U.S. Military Academy, Ice House, Mills Road at Howze Place, West Point, Orange County, NY

  19. Contextual view of building 505 showing west elevation from marsh; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view of building 505 showing west elevation from marsh; camera facing east. - Mare Island Naval Shipyard, Defense Electronics Equipment Operating Center, I Street, terminus west of Cedar Avenue, Vallejo, Solano County, CA

  20. Detail of main entry on east elevation; camera facing west. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of main entry on east elevation; camera facing west. - Mare Island Naval Shipyard, Defense Electronics Equipment Operating Center, I Street, terminus west of Cedar Avenue, Vallejo, Solano County, CA

  1. 3. Perspective view of west entrance to Gas House. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Perspective view of west entrance to Gas House. - Delaware, Lackawanna & Western Railroad, Scranton Yards, Gas House, 100 block of South Washington Avenue, west side, Scranton, Lackawanna County, PA

  2. 11. General layout of Erie tracks and roundhouse west of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. General layout of Erie tracks and roundhouse west of old Bergen Tunnel, looking west from Tonnel Avenue bridge, taken June 14, 1906 - Erie Railway, Bergen Hill Open Cut, Palisade Avenue to Tonnele Avenue, Jersey City, Hudson County, NJ

  3. 1. NORTH AND WEST SIDES OF BUILDING 346. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. NORTH AND WEST SIDES OF BUILDING 346. VIEW TO SOUTHEAST. - Rocky Mountain Arsenal, Storage Warehouse for Incendiary Clusters, 1659 feet South of December Seventh Avenue; 2060 feet West of D Street, Commerce City, Adams County, CO

  4. View of West end of central lift span truss web ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of West end of central lift span truss web of Tensaw River Bridge, showing web brace of lift girder superstructure, looking west - Tensaw River Lift Bridge, Spanning Tensaw River at U.S. Highway 90, Mobile, Mobile County, AL

  5. 340. Caltrans, Photographer October 14, 1935 "TUNNEL WEST PORTAL"; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    340. Caltrans, Photographer October 14, 1935 "TUNNEL - WEST PORTAL"; VIEW OF TUNNEL - WEST PORTAL' UNDER CONSTRUCTION SHOWING EXCAVATION OF TUNNEL AFTER POUR. 5-1669 - San Francisco Oakland Bay Bridge, Spanning San Francisco Bay, San Francisco, San Francisco County, CA

  6. VIEW OF THE WEST SIDE OF BUILDING 63, FACING EAST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF THE WEST SIDE OF BUILDING 63, FACING EAST. - Naval Computer & Telecommunications Area Station, Enlisted Men's Barracks, West corner of Central Street & Midway Drive, Wahiawa, Honolulu County, HI

  7. VIEW OF THE SOUTH AND WEST SIDES OF BUILDING 63, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF THE SOUTH AND WEST SIDES OF BUILDING 63, FACING NORTHEAST. - Naval Computer & Telecommunications Area Station, Enlisted Men's Barracks, West corner of Central Street & Midway Drive, Wahiawa, Honolulu County, HI

  8. View of east elevation along California Avenue; camera facing west. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of east elevation along California Avenue; camera facing west. - Mare Island Naval Shipyard, Rubber Shop, California Avenue, west side across from Dry Dock 1 near Ninth Street, Vallejo, Solano County, CA

  9. 1. VIEW OF WEST HEADWALL AND WING WALL, FROM BRIDGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW OF WEST HEADWALL AND WING WALL, FROM BRIDGE TO THE WEST, FACING EAST. - Cut Stone Bridge, Southern Pacific Railroad line spanning runoff channel at South Spruce Avenue, South San Francisco, San Mateo County, CA

  10. VIEW OF BRIDGE CANTILEVER THROUGH TRUSS CANTILEVER PORTAL ON WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF BRIDGE CANTILEVER THROUGH TRUSS CANTILEVER PORTAL ON WEST BANK SIDE LOOKING NORTHWEST. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  11. VIEW OF BRIDGE CANTILEVER THROUGH TRUSS CANTILEVER SECTION, LOOKING WEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF BRIDGE CANTILEVER THROUGH TRUSS CANTILEVER SECTION, LOOKING WEST. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  12. Front (west side) and south side of building Fitzsimons ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Front (west side) and south side of building - Fitzsimons General Hospital, Golf Course Waiting Shelter, Southwest area of Golf Course, 700 feet Northeast of intersection of West Harlow Avenue & Peoria Street, Aurora, Adams County, CO

  13. 76 FR 16603 - Hiawatha West Resource Advisory Committee; Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service Hiawatha West Resource Advisory Committee; Meeting AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The Hiawatha West Resource Advisory Committee will meet in Rapid...

  14. 2. NORTH AND WEST SIDES OF BUILDING 422. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. NORTH AND WEST SIDES OF BUILDING 422. VIEW TO SOUTHEAST. - Rocky Mountain Arsenal, Administration-Laboratory- Change House-Bomb Rail, 420 feet South of December Seventh Avenue; 530 feet West of D Street, Commerce City, Adams County, CO

  15. 127. INTERIOR, SIXTH FLOOR, WING 6100 WEST, SUITE 6000, ROOM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    127. INTERIOR, SIXTH FLOOR, WING 6100 WEST, SUITE 6000, ROOM 6156, OFFICE OF THE SECRETARY OF THE INTERIOR, WEST WALL - U.S. Department of the Interior, Eighteenth & C Streets Northwest, Washington, District of Columbia, DC

  16. 6. VIEW NORTHEAST, WEST END OF DAM DURING CONSTRUCTION OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW NORTHEAST, WEST END OF DAM DURING CONSTRUCTION OF FISHWAY, DECEMBER 1995, SHOWING REMOVAL OF PLANKING - Norwich Water Power Company, Dam, West bank of Shetucket River opposite Fourteenth Street, Greenville section, Norwich, New London County, CT

  17. General view looking west from eastern edge of complex. Kilns ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view looking west from eastern edge of complex. Kilns have been demolished but kiln stacks still stand. - Harbison-Walker Refractories Company, West end of Shirley Street, Mount Union, Huntingdon County, PA

  18. View of headframe, looking west from Powell Memorial Orphan ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of headframe, looking west from Powell Memorial - Orphan Lode Mine, Headframe, North of West Rim Road between Powell Point and Maricopa Point, South Rim, Grand Canyon Village, Coconino County, AZ

  19. 2. View facing north of west and south elevations of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View facing north of west and south elevations of Dixie Cotton Mill. The brick structure that forms the west elevation was added in 1913. - Dixie Cotton Mill, 710 Greenville Street, La Grange, Troup County, GA

  20. 1. TIP TOP MINE. WEST SIDE OF STRUCTURE. CAMERA POINTED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. TIP TOP MINE. WEST SIDE OF STRUCTURE. CAMERA POINTED EAST. - Florida Mountain Mining Sites, Tip Top Mine, West face Florida Mountain, approximately 150 feet below summit, Silver City, Owyhee County, ID

  1. 1. View looking northwest, showing Saybrook west breakwater and Lynde ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View looking northwest, showing Saybrook west breakwater and Lynde Point Lighthouse in the right background - Saybrook Breakwater Light, South tip of west end of Saybrook Breakwater, Old Saybrook, Middlesex County, CT

  2. Perspective, environmental view looking from the intersection of West Lanvale ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Perspective, environmental view looking from the intersection of West Lanvale Street and North Carrollton Avenue - Reformed Episcopal Church of the Rock of Ages, 1210 West Lanvale Street, Baltimore, Independent City, MD

  3. 1. Building 9 west elevation along Crawford Street. Adjoining Building ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Building 9 west elevation along Crawford Street. Adjoining Building 10 on right. View looking SEE. - John & James Dobson Carpet Mill (West Parcel), Building No. 9, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  4. 3. Building 7 north elevation (west end), showing loading area. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Building 7 north elevation (west end), showing loading area. View looking south. - John & James Dobson Carpet Mill (West Parcel), Building No. 7, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  5. VIEW OF NORTH SIDE OF BUILDING 23 FROM WEST EDGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF NORTH SIDE OF BUILDING 23 FROM WEST EDGE OF PARKING LOT, FACING SOUTHEAST - Roosevelt Base, Auditorium-Gymnasium, West Virginia Street between Richardson & Reeves Avenues, Long Beach, Los Angeles County, CA

  6. 5. NORTH AND WEST SIDES OF BUILDING 243. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. NORTH AND WEST SIDES OF BUILDING 243. VIEW TO SOUTHEAST. - Rocky Mountain Arsenal, Chlorine Production Cell Building, 405 feet South of December Seventh Avenue; 330 feet West of D Street, Commerce City, Adams County, CO

  7. 4. NORTH AND WEST SIDES OF BUILDING 242. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. NORTH AND WEST SIDES OF BUILDING 242. VIEW TO SOUTHEAST. - Rocky Mountain Arsenal, Chlorine Production Cell Building, 405 feet South of December Seventh Avenue; 330 feet West of D Street, Commerce City, Adams County, CO

  8. 6. WEST AND SOUTH SIDES OF BUILDING 243. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. WEST AND SOUTH SIDES OF BUILDING 243. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Chlorine Production Cell Building, 405 feet South of December Seventh Avenue; 330 feet West of D Street, Commerce City, Adams County, CO

  9. 3. SOUTH AND WEST SIDES OF BUILDING 242 (RIGHT) AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. SOUTH AND WEST SIDES OF BUILDING 242 (RIGHT) AND 243 (LEFT). VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Chlorine Production Cell Building, 405 feet South of December Seventh Avenue; 330 feet West of D Street, Commerce City, Adams County, CO

  10. 19. INTERIOR VIEW TO THE WEST OF THE NORTH OFFICE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. INTERIOR VIEW TO THE WEST OF THE NORTH OFFICE SPACE AT THE WEST END OF ROOM 101. - Nevada Test Site, Pluto Facility, Disassembly Building, Area 26, Wahmonie Flats, Cane Spring Road, Mercury, Nye County, NV

  11. 20. INTERIOR VIEW TO THE WEST OF THE SOUTH OFFICE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. INTERIOR VIEW TO THE WEST OF THE SOUTH OFFICE SPACE AT THE WEST END OF ROOM 101. - Nevada Test Site, Pluto Facility, Disassembly Building, Area 26, Wahmonie Flats, Cane Spring Road, Mercury, Nye County, NV

  12. 21. INTERIOR VIEW TO THE WEST OF EQUIPMENT ABOVE THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. INTERIOR VIEW TO THE WEST OF EQUIPMENT ABOVE THE OFFICE SPACE AT THE WEST END OF ROOM 101. - Nevada Test Site, Pluto Facility, Disassembly Building, Area 26, Wahmonie Flats, Cane Spring Road, Mercury, Nye County, NV

  13. Exterior view to the southeast of the west camera bunker ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Exterior view to the southeast of the west camera bunker outside the fenced facility area - Nevada Test Site, Test Cell C Facility, West Camera Bunker, Area 25, Jackass Flats, Road J, Mercury, Nye County, NV

  14. 21 CFR 866.3940 - West Nile virus serological reagents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3940 West Nile... detection aids in the clinical laboratory diagnosis of viral meningitis/encephalitis caused by West...

  15. 21 CFR 866.3940 - West Nile virus serological reagents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3940 West Nile... detection aids in the clinical laboratory diagnosis of viral meningitis/encephalitis caused by West...

  16. 21 CFR 866.3940 - West Nile virus serological reagents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3940 West Nile... detection aids in the clinical laboratory diagnosis of viral meningitis/encephalitis caused by West...

  17. 21 CFR 866.3940 - West Nile virus serological reagents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3940 West Nile... detection aids in the clinical laboratory diagnosis of viral meningitis/encephalitis caused by West...

  18. 21 CFR 866.3940 - West Nile virus serological reagents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3940 West Nile... detection aids in the clinical laboratory diagnosis of viral meningitis/encephalitis caused by West...

  19. 12. Relationship of est tool shed, west tool shed, residence, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Relationship of est tool shed, west tool shed, residence, claim house, and chicken house to each other and immediate surroundings, looking southeast - George Spangerberger Farmstead, 2012 West Illinois Avenue, South Hutchinson, Reno County, KS

  20. 4. VIEW OF WEST AND SOUTH FACADES OF BOILER HOUSE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW OF WEST AND SOUTH FACADES OF BOILER HOUSE, WITH COAL HOPPERS AT W EST, LOOKING NORTHEAST - Marvine Colliery, Boiler House No. 2, West side Boulevard Avenue, between East Parker Street & Route 380, Scranton, Lackawanna County, PA

  1. Detail of arched window by main entry on west elevation; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of arched window by main entry on west elevation; showing Stone Foundation; camera facing east. - Mare Island Naval Shipyard, Smithery, California Avenue, west side at California Avenue & Eighth Street, Vallejo, Solano County, CA

  2. 7. Historic American Buildings Survey February, 1953 LOOKING WEST AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey February, 1953 LOOKING WEST AT FOUNDATION WHICH IS CONTIGUOUS TO WEST WALL OF THE JONATHAN HAGER HOUSE - Jonathan Hager House (Foundation), Hagerstown, Washington County, MD

  3. 18. West room, second floor. View looking southeast. Center hall ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. West room, second floor. View looking southeast. Center hall visible through doorway. - Fort Hill Farm, Mansion, West of Staunton (Roanoke) River between Turkey & Caesar's Runs, Clover, Halifax County, VA

  4. 11. West room, first floor. View looking east. Front porch ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. West room, first floor. View looking east. Front porch visible through window. - Fort Hill Farm, Mansion, West of Staunton (Roanoke) River between Turkey & Caesar's Runs, Clover, Halifax County, VA

  5. VIEW OF POST OFFICE FROM THE INTERSECTION OF WEST MAXWELL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF POST OFFICE FROM THE INTERSECTION OF WEST MAXWELL BOULEVARD AND SOUTH MITCHELL STREET FACING SOUTHWEST. - Maxwell Air Force Base, Post Office, 61 West Maxwell Boulevard, Montgomery, Montgomery County, AL

  6. VIEW OF POST OFFICE FROM THE INTERSECTION OF WEST MAXWELL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF POST OFFICE FROM THE INTERSECTION OF WEST MAXWELL BOULEVARD AND SOUTH LEMAY PLAZA FACING SOUTHEAST. - Maxwell Air Force Base, Post Office, 61 West Maxwell Boulevard, Montgomery, Montgomery County, AL

  7. 253. 441 SOUTH NINETEENTH STREET, WEST FRONT AND SOUTH SIDE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    253. 441 SOUTH NINETEENTH STREET, WEST FRONT AND SOUTH SIDE, AND 1829, SOUTH FRONT AND WEST SIDE, TOWARD NORTHEAST - Russell Neighborhood, Bounded by Congress & Esquire Alley, Fifteenth & Twenty-first Streets, Louisville, Jefferson County, KY

  8. VIEW OF FURNACE NO. 2 AND CARPENTRY SHOP, LOOKING WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF FURNACE NO. 2 AND CARPENTRY SHOP, LOOKING WEST - Chambers Window Glass Company, Furnace No. 2, North of Drey (Nineteenth) Street, West of Constitution Boulevard, Arnold, Westmoreland County, PA

  9. 6. OVERALL OBLIQUE VIEW SOUTHSOUTHWEST, SHOWING NORTH & WEST FACADES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. OVERALL OBLIQUE VIEW SOUTH-SOUTHWEST, SHOWING NORTH & WEST FACADES WITH SHED ROOF BUILDING 8 JUTTING FROM NORTH FACADE OF WEST BAY. - Vulcan Crucible Steel Company, Building No. 3, 100 First Street, Aliquippa, Beaver County, PA

  10. North and west facades of crucible steel building; looking southeast ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North and west facades of crucible steel building; looking southeast - Bethlehem Steel Corporation, South Bethlehem Works, Crucible Steel Plant, Along Lehigh River, North of Fourth Street, West of Minsi Trail Bridge, Bethlehem, Northampton County, PA

  11. CAR SHOP TRANSFER TABLE, IN STORAGE AT WEST SIDE OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CAR SHOP TRANSFER TABLE, IN STORAGE AT WEST SIDE OF SHOPS, WEST END JOURNAL BEARING AND WHEELSET DETAIL, LOOKING NORTHEAST. - Southern Pacific, Sacramento Shops, Pitless Transfer Table, 111 I Street, Sacramento, Sacramento County, CA

  12. 4. GENERAL VIEW FROM THE WEST, SHOWING REFLECTING POOL IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. GENERAL VIEW FROM THE WEST, SHOWING REFLECTING POOL IN FOREGROUND (CLOSER VIEW) - Washington Monument, High ground West of Fifteenth Street, Northwest, between Independence & Constitution Avenues, Washington, District of Columbia, DC

  13. 3. GENERAL VIEW FROM THE WEST, SHOWING REFLECTING POOL IN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. GENERAL VIEW FROM THE WEST, SHOWING REFLECTING POOL IN FOREGROUND - Washington Monument, High ground West of Fifteenth Street, Northwest, between Independence & Constitution Avenues, Washington, District of Columbia, DC

  14. 2. DOWNSTREAM VIEW OF THE SOUTH CHANNEL DAM, LOOKING WEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. DOWNSTREAM VIEW OF THE SOUTH CHANNEL DAM, LOOKING WEST. - Washington Water Power Company Post Falls Power Plant, South Channel Dam, West of intersection of Spokane & Fourth Streets, Post Falls, Kootenai County, ID

  15. Rainbow Pool, Eastern portion of West Potomac Park; bounded by ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Rainbow Pool, Eastern portion of West Potomac Park; bounded by Elm Walks to the north and south, Seventeenth Street to the east and the Reflecting Pool to the west, Washington, District of Columbia, DC

  16. West Branch Pennsylvania Canal, Lock No. 34 Lock Keeper's House, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West Branch Pennsylvania Canal, Lock No. 34 Lock Keeper's House, South of State Route 664 along North bank of West Branch of Susquehanna River, 2,000 feet East of Jay Street Bridge, Lock Haven, Clinton County, PA

  17. 4. CENTER COURTYARD, FROM WEST SIDE OF SOUTHEAST PORTION OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. CENTER COURTYARD, FROM WEST SIDE OF SOUTHEAST PORTION OF BUILDING, LOOKING WEST. - Oakland Naval Supply Center, Administration Building-Dental Annex-Dispensary, Between E & F Streets, East of Third Street, Oakland, Alameda County, CA

  18. 1. SOUTH AND WEST SIDES OF BUILDING 254. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. SOUTH AND WEST SIDES OF BUILDING 254. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Caustic Fusion Building, 450 feet South of December Seventh Avenue; 900 feet West of D Street, Commerce City, Adams County, CO

  19. General view of central and west wings with loading dock ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view of central and west wings with loading dock from north - National Home for Disabled Volunteer Soldiers, Pacific Branch, Mess Hall, 11301 Wilshire Boulevard, West Los Angeles, Los Angeles County, CA

  20. 12. OVERVIEW TO WEST. RHODES DITCH RUNS ACROSS PHOTO TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. OVERVIEW TO WEST. RHODES DITCH RUNS ACROSS PHOTO TO THIS SIDE OF SCATTERED OAKS IN DISTANCE. - Natomas Ditch System, Rhodes Ditch, West of Bidwell Street, north of U.S. Highway 50, Folsom, Sacramento County, CA

  1. 2. OBLIQUE VIEW OF NORTHWEST CORNER OF WEST WING. THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. OBLIQUE VIEW OF NORTHWEST CORNER OF WEST WING. THE VAULT ROOM IS ATTACHED TO THE WEST WALL, SHOWN AT FAR RIGHT. - Oakland Army Base, General Purpose Administration Building, Chungking & Algiers Streets, Oakland, Alameda County, CA

  2. View of Boiler from west, Steam Engine for shop line ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of Boiler from west, Steam Engine for shop line shaft in foreground - East Broad Top Railroad & Coal Company, Machine Shop, State Route 994, West of U.S. Route 522, Rockhill Furnace, Huntingdon County, PA

  3. 7. Detail of west abutment, floor beam and stringer system; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Detail of west abutment, floor beam and stringer system; view looking west, southwest - St. Louis Avenue Viaduct, Spanning Kansas City Terminal Railroad at Beardsley Road & Bluff Street, Kansas City, Jackson County, MO

  4. 9. SOUTH END OF GENE PUMPING STATION LOOKING WEST WITH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. SOUTH END OF GENE PUMPING STATION LOOKING WEST WITH DELIVERY LINES IN BACKGROUND. - Gene Pump Plant, South of Gene Wash Reservoir, 2 miles west of Whitsett Pump Plant, Parker Dam, San Bernardino County, CA

  5. South and west elevations of tractor shed facing northeast ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South and west elevations of tractor shed facing northeast - Norris Farm, Tractor Shed-Combine Barn, .5 mile west of County Road 857 & .25 mile east of County Road 88/1, Cheat Neck, Monongalia County, WV

  6. Curve of Western Maryland Rail Trail west of Hancock, milepost ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Curve of Western Maryland Rail Trail west of Hancock, milepost 117 vicinity, looking west. - Western Maryland Railway, Cumberland Extension, Pearre to North Branch, from WM milepost 125 to 160, Pearre, Washington County, MD

  7. Building L west elevation oblique from cartway (between Buildings L ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Building L west elevation oblique from cartway (between Buildings L and M), also showing west elevation of Building J - Daniel F. Waters Germantown Dye Works, Building L, 37-55 East Wister Street, Philadelphia, Philadelphia County, PA

  8. 2. HISTORIC PHOTOGRAPH, VIEW TO WEST ROCKS ROAD BRIDGE, CA. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. HISTORIC PHOTOGRAPH, VIEW TO WEST ROCKS ROAD BRIDGE, CA. 1940. COLLECTION CONNECTICUT DEPARTMENT OF TRANSPORTATION. - Merritt Parkway, West Rocks Road Bridge, Spanning Merritt Parkway, Norwalk, Fairfield County, CT

  9. WEST PIER OF NORTH GATE (490 NORTH & 900 EAST), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEST PIER OF NORTH GATE (490 NORTH & 900 EAST), SALT LAKE CITY, UT. VIEW LOOKING SOUTH AT THE WEST PIER OF THE CEMETERY'S NORTH GATE. - Salt Lake City Cemetery, 200 N Street, Salt Lake City, Salt Lake County, UT

  10. Route No. 1 near west edge of Badlands Topographical Area, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Route No. 1 near west edge of Badlands Topographical Area, view to west - Route No. 1-Overton-Lake Mead Road, Between Overton Beach & Park Boundary, 6 miles south of Overton, Overton, Clark County, NV

  11. 8. Approach spans (two warren pony trusses), west side, detail ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Approach spans (two warren pony trusses), west side, detail of lower chords and pier no. 2 (west pier); looking south. - Bridge 4666, Minnesota Trunk Highway 19 spanning Minnesota River, North Redwood, Redwood County, MN

  12. Stuccoed building within greenhouse complex, north and west (front) sides, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Stuccoed building within greenhouse complex, north and west (front) sides, looking south towards building no. 121 (tennis courts) across W. Pennington Ave. - Fitzsimons General Hospital, Greenhouse, West Pennington Avenue, East of Building No. 139, Aurora, Adams County, CO

  13. 3. WEST AND SOUTH SIDES OF BUILDING 251. VIEW TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. WEST AND SOUTH SIDES OF BUILDING 251. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Chlorine Evaporator & Storage Building, 800 feet South of December Seventh Avenue; 600 feet West of D Street, Commerce City, Adams County, CO

  14. 4. Building 10 west elevation detail, showing window bays and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Building 10 west elevation detail, showing window bays and change in construction materials, south end of elevation. View looking SSE. - John & James Dobson Carpet Mill (West Parcel), Building No. 10, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  15. Front (south side) and west side of building. Fitzsimons ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Front (south side) and west side of building. - Fitzsimons General Hospital, Civilian Employee Garage, North end of North Hickey Street, 775 feet North-Northwest of intersection of North Hickey Street & West Loosley Avenue, Aurora, Adams County, CO

  16. West side of building Fitzsimons General Hospital, Civilian Employee ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West side of building - Fitzsimons General Hospital, Civilian Employee Housing, North end of North Hickey Street, 725 feet North-Northwest of intersection of North Hickey Street & West Loosley Avenue, Aurora, Adams County, CO

  17. Rear (north side) and west side of building. Fitzsimons ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Rear (north side) and west side of building. - Fitzsimons General Hospital, Civilian Employee Garage, North end of North Hickey Street, 775 feet North-Northwest of intersection of North Hickey Street & West Loosley Avenue, Aurora, Adams County, CO

  18. 3. General oblique view of west facade showing brick piers ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. General oblique view of west facade showing brick piers and industrial windows; view to southeast. - Champion-International Paper Company, Paper Machine Building, West bank of Spicket River at Canal Street, Lawrence, Essex County, MA

  19. 4. Detail of west facade showing brick piers and industrial ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Detail of west facade showing brick piers and industrial windows with metal sash and reinforced concrete lintels and sills. - Champion-International Paper Company, Paper Machine Building, West bank of Spicket River at Canal Street, Lawrence, Essex County, MA

  20. 75 FR 51183 - Fisheries Off West Coast States; Modifications of the West Coast Commercial and Recreational...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-19

    .... SUPPLEMENTARY INFORMATION: In the 2010 annual management measures for ocean salmon fisheries (75 FR 24482, May 5... the annual management measures for ocean salmon fisheries (75 FR 24482, May 5, 2010), the West...

  1. 75 FR 75639 - Fisheries Off West Coast States; Modifications of the West Coast Commercial and Recreational...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-06

    .... SUPPLEMENTARY INFORMATION: In the 2010 annual management measures for ocean salmon fisheries (75 FR 24482, May 5... the annual management measures for ocean salmon fisheries (75 FR 24482, May 5, 2010), the West...

  2. GeoPowering the West: Geothermal Energy--The West's Clean Energy Source

    SciTech Connect

    Not Available

    2005-01-01

    This ''calling card'' type brochure describes the GeoPowering the West (GPW) Initiative and activities, and lists critical contact information. This brochure serves as an introductory piece for anyone inquiring about the GPW initiative.

  3. Hydrogeology of the West Siberian Basin

    SciTech Connect

    Foley, M.G.; Bradley, D.J.; Cole, C.R.

    1996-08-01

    Nuclear fuel cycle activities of the former Soviet Union (FSU) have resulted in extensive radioactive contaminant releases to the environment in western Siberia. We are developing three-dimensional numerical models of the hydrogeology and potential contaminant migration in the West Siberian Basin. We have assumed that ground-water flow in the West Siberian Basin is topographically driven, with recharge to the basin occurring in the highlands on the west, east, and south, and internal discharge localized in numerous river valleys and lakes that ultimately discharge north to the ocean. We are modeling the regional hydrogeology as three-dimensional, steady-state, saturated flow that is recharged from above. We acquired topographic, geologic, hydrostratigraphic, hydrogeologic, and water-balance data for the West Siberian Basin and constructed a regional water table. We correlated and combined 70 different rock types derived from published descriptions of West Siberian Basin rocks into 17 rock types appropriate for assignment of hydrogeologic properties on the basis of spatial heterogeneity and constituent (i.e., sand, silt, and clay) diversity. Examination of resulting three-dimensional assemblages of rock types showed that they were consistent with published and inferred paleogeography and depositional processes. Calibrating the basin`s moisture balance (i.e., recharge and discharge) to the derived water table determined plausible input parameter values for unknowns such as hydraulic conductivities. The general directions of calculated ground-water flow suggest that major rivers act as discharge areas, with upwelling below the rivers extending down into the basement rocks, and that ground-water divides that penetrate the entire thickness of the model are evident between major rivers.

  4. The crustal thickness of West Antarctica

    NASA Astrophysics Data System (ADS)

    Chaput, J.; Aster, R. C.; Huerta, A.; Sun, X.; Lloyd, A.; Wiens, D.; Nyblade, A.; Anandakrishnan, S.; Winberry, J. P.; Wilson, T.

    2014-01-01

    P-to-S receiver functions (PRFs) from the Polar Earth Observing Network (POLENET) GPS and seismic leg of POLENET spanning West Antarctica and the Transantarctic Mountains deployment of seismographic stations provide new estimates of crustal thickness across West Antarctica, including the West Antarctic Rift System (WARS), Marie Byrd Land (MBL) dome, and the Transantarctic Mountains (TAM) margin. We show that complications arising from ice sheet multiples can be effectively managed and further information concerning low-velocity subglacial sediment thickness may be determined, via top-down utilization of synthetic receiver function models. We combine shallow structure constraints with the response of deeper layers using a regularized Markov chain Monte Carlo methodology to constrain bulk crustal properties. Crustal thickness estimates range from 17.0±4 km at Fishtail Point in the western WARS to 45±5 km at Lonewolf Nunataks in the TAM. Symmetric regions of crustal thinning observed in a transect deployment across the West Antarctic Ice Sheet correlate with deep subice basins, consistent with pure shear crustal necking under past localized extension. Subglacial sediment deposit thicknesses generally correlate with trough/dome expectations, with the thickest inferred subice low-velocity sediment estimated as ˜0.4 km within the Bentley Subglacial Trench. Inverted PRFs from this study and other published crustal estimates are combined with ambient noise surface wave constraints to generate a crustal thickness map for West Antarctica south of 75°S. Observations are consistent with isostatic crustal compensation across the central WARS but indicate significant mantle compensation across the TAM, Ellsworth Block, MBL dome, and eastern and western sectors of thinnest WARS crust, consistent with low density and likely dynamic, low-viscosity high-temperature mantle.

  5. Oil and gas potential of the Triassic in west Siberia

    SciTech Connect

    Bochkarev, V.S.; Kulakhmetov, N.KH.; Nesterov, I.I. )

    1993-09-01

    Permian-Triassic rocks are widely spread within the West Siberian basin, and they include volcanics, volcanoclastics, and clastics. Their thickness varies from tens of meters of 3000 m. Recently, three commercial oil pools have been discovered in Triassic effusive-sedimentary rocks. These discoveries, together with other geological and geochemical data, identify the Triassic complex as a major play. Oil-bearing intervals have been found in three different types of sequences; a fourth also may be prospective. The first type is represented by lacustrine-terrigenous sediments, which comprise oil-saturated sandstones interbedded with basalts (the Turin series). Oil influxes were obtained in the Yakhlinskaya and Triyurtin-skaya structures in the Shaim region. The second type is distinguished from the first by the presence of coal-bearing intervals in the upper part. Oil was produced in the Yerofeyev area of the Chelyabinsk garben. The third type differs from the other two by the presence of potassic rhyolites and dacites. Their age is not precisely dated, and supposedly they are of Permian age. The largest oil influxes have been obtained from fractured and eroded rocks of this type in the Rogozhnikovskaya and other places in the Krasnoleninsk region. Rhyodacites often underlie Turin basalts, but locally they occur in the upper part of the series. Triassic and Permian-Trissic rocks of the three types are overlain by Jurassic and Cretaceous rocks with a large break. The fourth type of section is completely terrigenous (Tampei series). It is developed in the northern part of west Siberia. Here Triassic sediments are overlain by the Jurassic complex without a break. According to well-log data, productive horizons occur at Urengoy and Beregovaya (in the Urengoy region).

  6. Assessing the continuity of the blue ice climate record at Patriot Hills, Horseshoe Valley, West Antarctica

    NASA Astrophysics Data System (ADS)

    Winter, Kate; Woodward, John; Dunning, Stuart A.; Turney, Chris S. M.; Fogwill, Christopher J.; Hein, Andrew S.; Golledge, Nicholas R.; Bingham, Robert G.; Marrero, Shasta M.; Sugden, David E.; Ross, Neil

    2016-03-01

    We use high-resolution ground-penetrating radar (GPR) to assess the continuity of the Blue Ice Area (BIA) horizontal climate record at Patriot Hills, Horseshoe Valley, West Antarctica. The sequence contains three pronounced changes in deuterium isotopic values at ~18 cal ka, ~12 cal ka, and ~8 cal ka. GPR surveys along the climate sequence reveal continuous, conformable dipping isochrones, separated by two unconformities in the isochrone layers, which correlate with the two older deuterium shifts. We interpret these unconformities as discontinuities in the sequence, rather than direct measures of climate change. Ice sheet models and Internal Layer Continuity Index plots suggest that the unconformities represent periods of erosion occurring, as the former ice surface was scoured by katabatic winds in front of mountains at the head of Horseshoe Valley. This study demonstrates the importance of high-resolution GPR surveys for investigating both paleoflow dynamics and interpreting BIA climate records.

  7. Assessment of undiscovered oil and gas resources of the West African Costal Province, West Africa

    USGS Publications Warehouse

    Brownfield, Michael E.; Charpentier, Ronald R.; Schenk, Christopher J.; Klett, Timothy R.; Cook, Troy A.; Pollastro, Richard M.

    2011-01-01

    The West African Coastal Province along the west African coastline recently was assessed for undiscovered, technically recoverable oil, natural gas, and natural gas liquids resources as part of the U.S. Geological Survey's USGS World Oil and Gas Assessment. Using a geology-based assessment methodology, the USGS estimated mean volumes of 3.2 billion barrels of oil, 23.63 trillion cubic feet of natural gas, and 721 million barrels of natural gas liquids.

  8. HIV Sequence Compendium 2015

    SciTech Connect

    Foley, Brian Thomas; Leitner, Thomas Kenneth; Apetrei, Cristian; Hahn, Beatrice; Mizrachi, Ilene; Mullins, James; Rambaut, Andrew; Wolinsky, Steven; Korber, Bette Tina Marie

    2015-10-05

    This compendium is an annual printed summary of the data contained in the HIV sequence database. We try to present a judicious selection of the data in such a way that it is of maximum utility to HIV researchers. Each of the alignments attempts to display the genetic variability within the different species, groups and subtypes of the virus. This compendium contains sequences published before January 1, 2015. Hence, though it is published in 2015 and called the 2015 Compendium, its contents correspond to the 2014 curated alignments on our website. The number of sequences in the HIV database is still increasing. In total, at the end of 2014, there were 624,121 sequences in the HIV Sequence Database, an increase of 7% since the previous year. This is the first year that the number of new sequences added to the database has decreased compared to the previous year. The number of near complete genomes (>7000 nucleotides) increased to 5834 by end of 2014. However, as in previous years, the compendium alignments contain only a fraction of these. A more complete version of all alignments is available on our website, http://www.hiv.lanl.gov/ content/sequence/NEWALIGN/align.html As always, we are open to complaints and suggestions for improvement. Inquiries and comments regarding the compendium should be addressed to seq-info@lanl.gov.

  9. Evolution of DNA sequencing.

    PubMed

    Tipu, Hamid Nawaz; Shabbir, Ambreen

    2015-03-01

    Sanger and coworkers introduced DNA sequencing in 1970s for the first time. It principally relied on termination of growing nucleotide chain when a dideoxythymidine triphosphate (ddTTP) was inserted in it. Detection of terminated sequences was done radiographically on Polyacrylamide Gel Electrophoresis (PAGE). Improvements that have evolved over time in original Sanger sequencing include replacement of radiography with fluorescence, use of separate fluorescent markers for each nucleotide, use of capillary electrophoresis instead of polyacrylamide gel electrophoresis and then introduction of capillary array electrophoresis. However, this technique suffered from few inherent limitations like decreased sensitivity for low level mutant alleles, complexities in analyzing highly polymorphic regions like Major Histocompatibility Complex (MHC) and high DNA concentrations required. Several Next Generation Sequencing (NGS) technologies have been introduced by Roche, Illumina and other commercial manufacturers that tend to overcome Sanger sequencing limitations and have been reviewed. Introduction of NGS in clinical research and medical diagnostics is expected to change entire diagnostic approach. These include study of cancer variants, detection of minimal residual disease, exome sequencing, detection of Single Nucleotide Polymorphisms (SNPs) and their disease association, epigenetic regulation of gene expression and sequencing of microorganisms genome.

  10. Holocene mangrove swamps of West Africa sedimentology and soils

    NASA Astrophysics Data System (ADS)

    Marius, C.; Lucas, J.

    The mangrove swamps of West African Coast belong to the Atlantic type which is characterized by a small number of species. They colonize tidal environments which are dissected by numerous meandering tidal channels and are presently subject to a low rate of sediment accumulation. The mangrove vegetation exhibits a characteristic zonation pattern that basically reflects the adaptation of the various species to saline conditions. The typical zonation sequence is: Rhizophora racemosa (or Rh. mangle), Rh. mangle + Avicennia africana, Avicennia, flooded tanne, barren tanne, herbaceous tanne. The tannes are generated by aridic climatic conditions, heavy soil and water salt content, and are, in a way a peculiar feature of mangrove swamps in West Africa. The sediment colonized by the mangroves is relatively homogenous. Mineralogically, they are dominated by quartz and clay to which are associated halite, pyrite and jarosite. The clay suite is mainly composed of smectite and kaolinite. Smectite is predominant in the inlet areas and is replaced inland by kaolinite. Chemically, the sediments contain very low amounts of Ca, bases and trace elements. The mangrove swamp floodwaters have a chemical composition similar to that of seawater. It is dominated by sodium and chloride. Morphologically, the ripening of the soils appears with a chestnut mash colour horizon and buttery consistency in relation with the decomposition of fibrous roots of Rhizophora and also with pale yellow jarosite mottles in the top horizons of the tanne profiles due to the oxidation of pyrine. The two main properties of the mangrove soils of West Africa are acidity and salinity; the first is related to the high content of sulphur and the second to the sea influence. The acidity has to be connected mainly to the Rhizophora vegetation whose the root system is a real trap for catching the pyrites resulting from the reduction of the sulphates of sea water by the sulphate reducing bacteria, in a reduced

  11. Whole genome sequence analysis of Mycobacterium suricattae.

    PubMed

    Dippenaar, Anzaan; Parsons, Sven David Charles; Sampson, Samantha Leigh; van der Merwe, Ruben Gerhard; Drewe, Julian Ashley; Abdallah, Abdallah Musa; Siame, Kabengele Keith; Gey van Pittius, Nicolaas Claudius; van Helden, Paul David; Pain, Arnab; Warren, Robin Mark

    2015-12-01

    Tuberculosis occurs in various mammalian hosts and is caused by a range of different lineages of the Mycobacterium tuberculosis complex (MTBC). A recently described member, Mycobacterium suricattae, causes tuberculosis in meerkats (Suricata suricatta) in Southern Africa and preliminary genetic analysis showed this organism to be closely related to an MTBC pathogen of rock hyraxes (Procavia capensis), the dassie bacillus. Here we make use of whole genome sequencing to describe the evolution of the genome of M. suricattae, including known and novel regions of difference, SNPs and IS6110 insertion sites. We used genome-wide phylogenetic analysis to show that M. suricattae clusters with the chimpanzee bacillus, previously isolated from a chimpanzee (Pan troglodytes) in West Africa. We propose an evolutionary scenario for the Mycobacterium africanum lineage 6 complex, showing the evolutionary relationship of M. africanum and chimpanzee bacillus, and the closely related members M. suricattae, dassie bacillus and Mycobacterium mungi.

  12. Whole genome sequence analysis of Mycobacterium suricattae.

    PubMed

    Dippenaar, Anzaan; Parsons, Sven David Charles; Sampson, Samantha Leigh; van der Merwe, Ruben Gerhard; Drewe, Julian Ashley; Abdallah, Abdallah Musa; Siame, Kabengele Keith; Gey van Pittius, Nicolaas Claudius; van Helden, Paul David; Pain, Arnab; Warren, Robin Mark

    2015-12-01

    Tuberculosis occurs in various mammalian hosts and is caused by a range of different lineages of the Mycobacterium tuberculosis complex (MTBC). A recently described member, Mycobacterium suricattae, causes tuberculosis in meerkats (Suricata suricatta) in Southern Africa and preliminary genetic analysis showed this organism to be closely related to an MTBC pathogen of rock hyraxes (Procavia capensis), the dassie bacillus. Here we make use of whole genome sequencing to describe the evolution of the genome of M. suricattae, including known and novel regions of difference, SNPs and IS6110 insertion sites. We used genome-wide phylogenetic analysis to show that M. suricattae clusters with the chimpanzee bacillus, previously isolated from a chimpanzee (Pan troglodytes) in West Africa. We propose an evolutionary scenario for the Mycobacterium africanum lineage 6 complex, showing the evolutionary relationship of M. africanum and chimpanzee bacillus, and the closely related members M. suricattae, dassie bacillus and Mycobacterium mungi. PMID:26542221

  13. Mitochondrial DNA analysis of hybridization between sympatric white-tailed deer and mule deer in west Texas.

    PubMed

    Carr, S M; Ballinger, S W; Derr, J N; Blankenship, L H; Bickham, J W

    1986-12-01

    Sympatric populations of white-tailed deer and mule deer (Odocoileus virginianus and Odocoileus hemionus, respectively) on a west Texas ranch share a common mitochondrial DNA restriction map genotype. Phylogenetic analysis indicates that this genotype is more characteristic of O. virginianus than of O. hemionus. The genotype of west Texas deer differs from that of O. virginianus from South Carolina by five mutational events (1.3% sequence divergence), whereas it differs from that of O. hemionus from California by 17 events (5.5% divergence). We suggest that interspecies hybridization has occurred, primarily between mule deer bucks and white-tailed deer does, with preferential absorption of hybrid offspring into the mule deer gene pool. Introgressive hybridization may be involved in ongoing displacement of mule deer by white-tailed deer in west Texas.

  14. West Nile Virus Encephalitis and Myocarditis in Wolf and Dog

    PubMed Central

    Heinz-Taheny, Kathleen; Osborne, Tanasa S.; Novak, Robert J.; Lewis, Beth A.; Firth, Margaret L.

    2003-01-01

    In the third season (2002) of the West Nile virus epidemic in the United States, two canids (wolf and dog) were diagnosed with West Nile virus encephalitis and myocarditis with similarities to known affected species (humans, horses, and birds). The West Nile virus infections were confirmed by immunohistochemistry and polymerase chain reaction. PMID:14609468

  15. 4. WEST PORTION OF SOUTH SIDE, OBLIQUE VIEW, FROM APPROXIMATELY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. WEST PORTION OF SOUTH SIDE, OBLIQUE VIEW, FROM APPROXIMATELY 30 FEET WEST OF SOUTHWEST CORNER, LOOKING EAST, WITH WEST SIDE OF BUILDING 123 AT RIGHT BEYOND LIGHTING POLE. - Oakland Naval Supply Center, Pier Transit Shed, South of D Street between First & Second Streets, Oakland, Alameda County, CA

  16. 14. VIEW OF WEST WALL OF CLEAN ROOM (102) SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. VIEW OF WEST WALL OF CLEAN ROOM (102) SHOWING VIEWING WINDOWS IN WEST FALSE PARTION WALL, WEST WALL OF CLEAN ROOM (102), AND ROLLS OF PLASTIC WRAP FOR COVERING CLEANED FAIRING ASSEMBLY - Vandenberg Air Force Base, Space Launch Complex 3, Vehicle Support Building, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

  17. West Harlem Walk (Hudson River Valley Greenway) beneath Henry Hudson ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    West Harlem Walk (Hudson River Valley Greenway) beneath Henry Hudson Parkway (HHP) Viaduct at West 155th Street vicinity, with Palisades, George Washington Bridge, and Little Red Lighthouse (visible to left of bridge tower) in background, looking northeast. - Henry Hudson Parkway, Extending 11.2 miles from West 72nd Street to Bronx-Westchester border, New York County, NY

  18. 129. VIEW OF WEST SIDE OF ACID TRUCK ROOM (113), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    129. VIEW OF WEST SIDE OF ACID TRUCK ROOM (113), LSB (BLDG. 770). BAY DOOR OPENS TO FUEL APRON ON WEST SIDE. BRICK FLOOR DATES FROM AGENA PERIOD WHEN ACID WAS STORED IN THE ROOM. - Vandenberg Air Force Base, Space Launch Complex 3, Launch Pad 3 West, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

  19. State Education Finance and Governance Profile: West Virginia

    ERIC Educational Resources Information Center

    Orkodashvili, Mariam

    2010-01-01

    This article presents the state education finance and governance profile of West Virginia. West Virginia is the 41st largest state in the United States, with an area of 24,231 square miles. The total population of West Virginia in 2008 was 1,814,468, making it the 37th most populous state in the United States. Current expenditures per pupil are…

  20. 77 FR 17561 - West Virginia Disaster #WV-00023

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... ADMINISTRATION West Virginia Disaster WV-00023 AGENCY: Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for the State of West Virginia..., Marion, Wayne. Contiguous Counties (Economic Injury Loans Only): West Virginia: Boone, Cabell,...

  1. 76 FR 44302 - West Virginia Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-25

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service West Virginia Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The West Virginia Resource Advisory Committee will meet in Elkins, West Virginia....

  2. 75 FR 41246 - West Virginia Disaster Number WV-00020

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... ADMINISTRATION West Virginia Disaster Number WV-00020 AGENCY: U.S. Small Business Administration. ACTION... of West Virginia (FEMA-1918-DR), dated 06/24/2010. Incident: Severe storms, flooding, mudslides, and... of West Virginia, dated 06/24/2010 is hereby amended to establish the incident period for...

  3. 77 FR 69490 - West Virginia; Emergency and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-19

    ... SECURITY Federal Emergency Management Agency West Virginia; Emergency and Related Determinations AGENCY... declaration of an emergency for the State of West Virginia (FEMA-3358-EM), dated October 29, 2012, and related... determined that the emergency conditions in the State of West Virginia resulting from Hurricane...

  4. 78 FR 51199 - West Virginia; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-20

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-4132-DR), dated July 26, 2013...''), as follows: I have determined that the damage in certain areas of the State of West...

  5. 77 FR 25225 - West Virginia Disaster Number WV-00027

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-27

    ... ADMINISTRATION West Virginia Disaster Number WV-00027 AGENCY: U.S. Small Business Administration. ACTION... of West Virginia (FEMA--4061--DR), dated 03/22/2012. Incident: Severe storms, flooding, mudslides and... West Virginia, dated 03/22/2012 is hereby amended to include the following areas as adversely...

  6. 75 FR 41245 - West Virginia Disaster Number WV-00018

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... ADMINISTRATION West Virginia Disaster Number WV-00018 AGENCY: U.S. Small Business Administration. ACTION... Assistance Only for the State of WEST VIRGINIA (FEMA-1903-DR), dated 04/23/2010. Incident: Severe Winter... disaster declaration for Private Non-Profit organizations in the State of WEST VIRGINIA, dated...

  7. 76 FR 27634 - West Virginia Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service West Virginia Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meetings. SUMMARY: The West Virginia Resource Advisory Committee will meet in Elkins, West Virginia....

  8. 75 FR 18517 - West Virginia; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-12

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-1893-DR), dated March 29... Act''), as follows: I have determined that the damage in certain areas of the State of West...

  9. 77 FR 47908 - West Virginia Disaster Number WV-00028

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-10

    ... ADMINISTRATION West Virginia Disaster Number WV-00028 AGENCY: U.S. Small Business Administration. ACTION... Assistance Only for the State of West Virginia (FEMA-4071-DR), dated 07/23/2012. Incident: Severe Storms and... disaster declaration for Private Non-Profit organizations in the State of West Virginia, dated...

  10. 77 FR 41193 - West Virginia; Emergency and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-12

    ... SECURITY Federal Emergency Management Agency West Virginia; Emergency and Related Determinations AGENCY... declaration of an emergency for the State of West Virginia (FEMA-3345-EM), dated June 30, 2012, and related... emergency conditions in the State of West Virginia resulting from severe storms beginning on June 29,...

  11. 75 FR 41246 - West Virginia Disaster Number WV-00020

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-15

    ... ADMINISTRATION West Virginia Disaster Number WV-00020 AGENCY: U.S. Small Business Administration. ACTION... of West Virginia (FEMA-1918-DR), dated 06/ 24/2010. Incident: Severe storms, flooding, mudslides, and... West Virginia, dated 06/24/2010 is hereby amended to include the following areas as adversely...

  12. 75 FR 35103 - West Virginia Disaster Number WV-00015

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... ADMINISTRATION West Virginia Disaster Number WV-00015 AGENCY: Small Business Administration. ACTION: Amendment 1... Only for the State of West Virginia (FEMA-1881-DR), dated 03/02/2010. Incident: Severe winter storm and... Private Non-Profit organizations in the State of West Virginia, dated 03/02/2010, is hereby amended...

  13. 75 FR 20401 - West Virginia Disaster Number WV-00017

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-19

    ... ADMINISTRATION West Virginia Disaster Number WV-00017 AGENCY: U.S. Small Business Administration. ACTION... Assistance Only for the State of West Virginia (FEMA-1893-DR), dated 03/29/2010. Incident: Severe Storms... major disaster declaration for Private Non-Profit organizations in the State of West Virginia, dated...

  14. 75 FR 46902 - West Virginia Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-04

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service West Virginia Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The West Virginia Resource Advisory Committee will meet in Elkins, West Virginia....

  15. 75 FR 63436 - West Virginia Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF AGRICULTURE Forest Service West Virginia Resource Advisory Committee AGENCY: Forest Service, USDA. ACTION: Notice of meeting. SUMMARY: The West Virginia Resource Advisory Committee will meet in Elkins, West Virginia....

  16. 75 FR 24756 - West Virginia Disaster Number WV-00017

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-05

    ... ADMINISTRATION West Virginia Disaster Number WV-00017 AGENCY: U.S. Small Business Administration. ACTION... Assistance Only for the State of West Virginia (FEMA--1893--DR), dated 03/29/2010. Incident: Severe Storms... major disaster declaration for Private Non-Profit organizations in the State of West Virginia, dated...

  17. 75 FR 38155 - West Virginia Disaster #WV-00020

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... ADMINISTRATION West Virginia Disaster WV-00020 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for the State of WEST...Dowell, Mingo, Wyoming. Contiguous Counties (Economic Injury Loans Only): West Virginia: Boone,...

  18. 78 FR 14148 - West Virginia Disaster #WV-00030

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-04

    ... ADMINISTRATION West Virginia Disaster WV-00030 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of West Virginia... adversely affected by the disaster: Primary Counties: Nicholas. Contiguous Counties: West Virginia:...

  19. 78 FR 55770 - West Virginia Disaster #WV-00032

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-11

    ... ADMINISTRATION West Virginia Disaster WV-00032 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of West Virginia... adversely affected by the disaster: Primary Counties: Roane. Contiguous Counties: West Virginia:...

  20. 77 FR 76061 - West Virginia; Major Disaster and Related Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-26

    ... SECURITY Federal Emergency Management Agency West Virginia; Major Disaster and Related Determinations... Presidential declaration of a major disaster for the State of West Virginia (FEMA-4093-DR), dated November 27... West Virginia resulting from Hurricane Sandy during the period of October 29 to November 8, 2012, is...