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Sample records for group committee c12

  1. 15 CFR 2002.1 - Trade Policy Committee Review Group.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Trade Policy Committee Review Group. 2002.1 Section 2002.1 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED STATES TRADE REPRESENTATIVE OPERATION OF COMMITTEES § 2002.1 Trade Policy...

  2. Executive Committee Working Group: Women in Astronomy

    NASA Astrophysics Data System (ADS)

    Primas, Francesca; Maddison, Sarah; Primas, Francesca; Aerts, Conny; Clayton, Geoffrey; Combes, Françoise; Elmegreen, Debra; Feretti, Luigina; Jog, Chanda; Kobayashi, Chiaki; Lazzaro, Daniela; Liang, Yanchun; Mandrini, Cristina; Mathews, Brenda; Rovira, Marta

    2016-04-01

    The gender† dimension of science and technology has become one of the most important and debated issues worldwide, impacting society at every level. A variety of international initiatives on the subject have been undertaken, including the continued monitoring of the status of women in science by Unesco Institute for Statistics (UIS) or the annual reports ``Education at a Glance'' by the Organization for Economic Co-operation and Development (OECD) as well as field-related working groups and networking in order to collect data in a consistent manner. The majority of the international organizations have made clear statements about their discrimination policies (independently of their main field(s) of action), including the International Council for Science whose regulations are followed by the IAU. Gender equality at large is one of the eight United Nations Millennium Development Goals, which clearly calls for action related to science, technology and gender.

  3. 75 FR 51525 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-20

    ... Federal Railroad Administration Railroad Safety Advisory Committee (RSAC); Working Group Activity Update... of Railroad Safety Advisory Committee (RSAC) Working Group Activities. SUMMARY: The FRA is updating its announcement of RSAC's Working Group activities to reflect its current status. FOR...

  4. The TSCA interagency testing committee`s approaches to screening and scoring chemicals and chemical groups: 1977-1983

    SciTech Connect

    Walker, J.D.

    1990-12-31

    This paper describes the TSCA interagency testing committee`s (ITC) approaches to screening and scoring chemicals and chemical groups between 1977 and 1983. During this time the ITC conducted five scoring exercises to select chemicals and chemical groups for detailed review and to determine which of these chemicals and chemical groups should be added to the TSCA Section 4(e) Priority Testing List. 29 refs., 1 fig., 2 tabs.

  5. EarthCube's Governance Working Group Steering Committee presents roadmap

    NASA Astrophysics Data System (ADS)

    Bowring, James F.; Pearthree, Genevieve M.

    2012-10-01

    June 2012 EarthCube Charrette;Washington, D. C., 12-14 June 2012 EarthCube is a U.S. National Science Foundation (NSF) initiative sponsored by the Directorate for Geosciences and the Office of Cyberinfrastructure to transform the conduct of research through open, community- guided development of cyberinfrastructure across the geosciences. EarthCube recently held its second organizational charrette (collaborative design event), with the objective of engaging its 190 physical and 60 remote attendees in discussions and workshops on developing EarthCube. One goal of the charrette was to review and integrate draft roadmaps produced by four NSF- funded Community Groups (Governance, Data, Semantics, and Workflow) and five Concept Awards (Brokering, Earth System Models, Layered Architecture, Interoperability, and Web Services), which emerged from the first charrette, held in November 2011. The roadmaps are the culmination of 6 months of research, community outreach, and deliberations in virtual and physical meetings; they identify initial EarthCube stakeholders and cyberinfrastructure components, in addition to key issues related to advancing EarthCube.

  6. 15 CFR 2002.1 - Trade Policy Committee Review Group.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .... 2002.1 Section 2002.1 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED STATES TRADE REPRESENTATIVE OPERATION OF COMMITTEES § 2002.1 Trade Policy Committee..., December 11, 1971 (15 CFR 2002.1), is abolished and there is hereby established as a subordinate body of...

  7. 15 CFR 2002.1 - Trade Policy Committee Review Group.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    .... 2002.1 Section 2002.1 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED STATES TRADE REPRESENTATIVE OPERATION OF COMMITTEES § 2002.1 Trade Policy Committee..., December 11, 1971 (15 CFR 2002.1), is abolished and there is hereby established as a subordinate body of...

  8. 15 CFR 2002.1 - Trade Policy Committee Review Group.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    .... 2002.1 Section 2002.1 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED STATES TRADE REPRESENTATIVE OPERATION OF COMMITTEES § 2002.1 Trade Policy Committee..., December 11, 1971 (15 CFR 2002.1), is abolished and there is hereby established as a subordinate body of...

  9. 15 CFR 2002.1 - Trade Policy Committee Review Group.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... 2002.1 Section 2002.1 Commerce and Foreign Trade Regulations Relating to Foreign Trade Agreements OFFICE OF THE UNITED STATES TRADE REPRESENTATIVE OPERATION OF COMMITTEES § 2002.1 Trade Policy Committee..., December 11, 1971 (15 CFR 2002.1), is abolished and there is hereby established as a subordinate body of...

  10. 75 FR 56055 - Advisory Committee and Species Working Group Technical Advisor Appointments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-15

    ... National Oceanic and Atmospheric Administration RIN 0648-XY91 Advisory Committee and Species Working Group... soliciting nominations for technical advisors to the Advisory Committee's species working groups. DATES... technical advisors to a species working group should be sent to Ms. Rachel O'Malley, Office of...

  11. 77 FR 43808 - Advisory Committee and Species Working Group Technical Advisor Appointment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-26

    ... National Oceanic and Atmospheric Administration RIN 0648-XC124 Advisory Committee and Species Working Group... nominations for technical advisors to the Advisory Committee's species working groups. DATES: Nominations must... working groups for the purpose of providing advice and recommendations to the U.S. Commissioners and...

  12. Understanding the functions and operations of data monitoring committees: Survey and focus group findings.

    PubMed

    Calis, Karim A; Archdeacon, Patrick; Bain, Raymond P; Forrest, Annemarie; Perlmutter, Jane; DeMets, David L

    2017-02-01

    The use of data monitoring committees in the conduct of clinical trials has increased and evolved, but there is a lack of published information on when data monitoring committees are needed and utilized, the acceptable range of data monitoring committee practices, and appropriate qualifications of data monitoring committee members. To gain a better understanding of data monitoring committee operations and areas for improvement, the Clinical Trials Transformation Initiative conducted a survey and set of focus groups. A total of 143 respondents completed the online survey: 76 data monitoring committee members, 52 sponsors involved with organization of data monitoring committees, and 15 statistical data analysis center representatives. There were 42 focus group participants, including data monitoring committee members; patients and/or patient advocate data monitoring committee members; institutional review board and US Food and Drug Administration representatives; industry, government, and non-profit sponsors; and statistical data analysis center representatives. Participants indicated that the primary responsibility of a data monitoring committee is to be an independent advisory body representing the interests of trial participants by assessing the risk and benefit ratio in ongoing trials. They noted that data monitoring committees must have access to unmasked data in order to perform this role. No clear consensus emerged regarding specific criteria for requiring a data monitoring committee for a given trial, and some participants felt data monitoring committees may be overused. Respondents offered suggestions for the data monitoring committee charter and communications with sponsors, institutional review boards, and regulators. Overall, data monitoring committee members reported that they are able to function independently and their recommendations are almost always accepted by the sponsor. Participants indicated that there are no standards or guidelines pertaining

  13. 76 FR 30722 - Meeting of the National Vaccine Advisory Committee; Vaccine Safety Working Group

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-26

    ... HUMAN SERVICES Meeting of the National Vaccine Advisory Committee; Vaccine Safety Working Group AGENCY... Department of Health and Human Services (DHHS) is hereby giving notice that the Vaccine Safety Working Group (VSWG) of the National Vaccine Advisory Committee (NVAC) will hold a meeting. The meeting is open to the...

  14. Theme: The Role of the Teacher in Using Advisory Committees and Other Stakeholder Groups.

    ERIC Educational Resources Information Center

    Agricultural Education Magazine, 2003

    2003-01-01

    Ten theme articles discuss the following: the need for and value of advisory committees in agricultural education; working with advisory committees, FFA alumni, and parent groups; how to define and choose stakeholders; teacher retention; the experiences of a first-year teacher; the value of the Veterinarian Care Council; and managing advisory…

  15. 76 FR 26771 - NASA Advisory Council; Task Group of the Science Committee; Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-09

    ... SPACE ADMINISTRATION NASA Advisory Council; Task Group of the Science Committee; Meeting AGENCY: National Aeronautics and Space Administration. ACTION: Notice of meeting. SUMMARY: In accordance with the Federal Advisory Committee Act, Public Law 92-463, as amended, the National Aeronautics and...

  16. 76 FR 21073 - NASA Advisory Council; Task Group of the Science Committee; Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-14

    ... SPACE ADMINISTRATION NASA Advisory Council; Task Group of the Science Committee; Meeting AGENCY: National Aeronautics and Space Administration. ACTION: Notice of meeting. SUMMARY: In accordance with the Federal Advisory Committee Act, Public Law 92-463, as amended, the National Aeronautics and...

  17. Committees

    NASA Astrophysics Data System (ADS)

    2012-11-01

    -RESIGA Politehnica University of Timisoara Romania Kazuhiro TANAKA Kyushu Institute of Technology Japan Yoshinobu TSUJIMOTO Osaka University Japan Local Organizing Committee Chairman Yulin WU Tsinghua University Beijing Executive Chairman Zhengwei WANG Tsinghua University Beijing Members Shuliang CAO Tsinghua University Beijing Cichang CHEN South West University of Petroleum Chengdu Hongxun CHEN Shanghai University Shanghai Jiang DAI China Sanxia General Co Yichang Huashu DOU National University of Singapore Singapore Fengqin HAN Huanan University of Sci & Tech Guangzhou Kun LI Hefei Inst of General Machinery Hefei Rennian LI Lanzhou University of Sci & Tech Lanzhou Wanhong LI National Natural Science Foundation of China Beijing Chao LIU Yangzhou University Yangzhou Li LU China Inst of Water Resources and Hydropower Research Beijing Xingqi LUO Xi'an University of Tech Xi'an Zhenyue MA Dalian University of Sci & Tech Dalian Jiegang MU Zhejiang University of Tech Hangzhou Daqing QIN Harbin Electric Machinery Group Harbin Fujun WANG China Agriculture University Beijing Guoyu WANG Beijing Institute of Technology (BIT) Beijing Leqin WANG Zhejiang University Hangzhou Yuzhen WU NERCSPV Beijing Hongyuan XU Tsinghua University Beijing Jiandong YANG Wuhan University Wuhan Minguan YANG Jiangsu University Zhenjiang Shouqi YUAN Jiangsu University Zhenjiang Lefu ZHANG Harbin Electric Machinery Group Harbin Lixiang ZHANG Yunnan University of Sci & Tech Kunming Shengchang ZHANG Zhejiang University of Tech Hangzhou Kun ZHAO China Water & Electric Consulting Corp Beijing Yuan ZHENG Hehai University Nanjing Jianzhong ZHOU Huazhong University of Sci & Tech Wuhan Lingjiu ZHOU China Agriculture University Beijing Hongwu ZHU China Petroleum University Beijing Zuchao ZHU Zhejiang Sci-Tech University Hangzhou Secretaries Shuhong LIU (Academic), liushuhong@tsinghua.edu.cn Xianwu LUO (Registration), luoxw@tsinghua.edu.cn Baoshan ZHU (Finance), bszhu@mail.tsinghua.edu.cn

  18. 76 FR 58078 - Thirteenth Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-19

    ... Federal Aviation Administration Thirteenth Meeting: RTCA Special Committee 214: Working Group 78.... ACTION: Notice of RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data... Special Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services. DATES:...

  19. 72 FR 7872 - Pesticide Program Dialogue Committee: Azinphos-methyl Transition Issues Work Group, Spray Drift...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2007-02-21

    ... AGENCY Pesticide Program Dialogue Committee: Azinphos-methyl Transition Issues Work Group, Spray Drift Work Group, and Registration Review Implementation Work Group; Notice of Public Meetings AGENCY...) will hold public meetings for three PPDC Work Groups: the Azinphos-methyl (AZM) Transition Issues...

  20. CTSA Consortium Consensus Scientific Review Committee (SRC) Working Group Report on the SRC Processes

    PubMed Central

    Buse, John B.; Califf, Robert M.; Carter, Robert; Cooper, Dan M.; Davis, Jonathan; Ford, Daniel E.; Galassetti, Pietro; Guay‐Woodford, Lisa; Huggins, Gordon S.; Kasper, Amanda; Kieburtz, Karl; Kirby, Aaron; Klein, Andreas K.; Kline, Joel; O’ Neill, Robert T.; Rape, Marie; Reichgott, Douglas J.; Rojevsky, Svetlana; Rosenthal, Gary E.; Rubinstein, Eric P.; Shepherd, Amy; Stacy, Mark; Terrin, Norma; Wallace, Mark; Welch, Lisa

    2015-01-01

    Abstract Human research projects must have a scientifically valid study design, analytic plan, and be operationally feasible in order to be successfully completed and thus to have translational impact. To ensure this, institutions that conduct clinical research should have a scientific review process prior to submission to the Institutional Review Committee (IRB). This paper reports the Clinical and Translational Science Award (CTSA) Consortium Scientific Review Committee (SRC) Consensus Working Group's proposed framework for a SRC process. Recommendations are provided for institutional support and roles of CTSAs, multisite research, criteria for selection of protocols that should be reviewed, roles of committee members, application process, and committee process. Additionally, to support the SCR process effectively, and to ensure efficiency, the Working Group recommends information technology infrastructures and evaluation metrics to determine outcomes are provided. PMID:26184433

  1. 77 FR 3765 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... Group and Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that members... outreach efforts. Entergy Regional State Committee Working Group and Stakeholders Meeting January 26,...

  2. 77 FR 21979 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Working Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-12

    ... Working Group and Stakeholder Meeting The Federal Energy Regulatory Commission (Commission) hereby gives... Commission's ongoing outreach efforts. Entergy Regional State Committee Working Group and Stakeholder...

  3. Executive committee report: geotechnical instrumentation working group meeting

    SciTech Connect

    Wilder, D.G.; Rogue, F.; Beloff, W.R.; Binnall, E.; Gregory, E.C.

    1982-04-26

    Responding to the widespread need for the geotechnical community to discuss instrumentation for nuclear waste repositories, a meeting was held December 2 and 3, 1981, in Denver, Colorado. This report gives the group's consensus recommendations to aid in making decisions for development of instrumentation for future repository work. The main conclusions of the working group meeting were as follows: (1) monitoring of geotechnical parameters in nuclear waste repositories will be necessary to meet licensing requirements; (2) currently available instruments are underdeveloped for this monitoring; (3) research and development to provide adequate instrumentation will need to be performed under federal sponsorship by national laboratories, universities, contractors, and consultants; and (4) a NASA-type reliability program is needed to meet the quality assurance, durability, calibration, and time schedule demands of geotechnical instrumentation development. This will require significant financial commitments from the federal sector.

  4. Committees

    NASA Astrophysics Data System (ADS)

    Williams, Gwyn P.; Revesz, Peter; Arp, Uwe

    2014-03-01

    Conference Chairs NameOrganization Gwyn Williams Jefferson Lab Peter ReveszCornell High Energy Synchrotron Source Uwe ArpSynchrotron Ultraviolet Radiation Facility Programme Committee NameOrganization Alastair MacDowellAdvanced Light Source Tom ToellnerAdvanced Photon Source Amitava D RoyCenter for Advanced Microstructures and Devices Tom EllisCanadian Light Source Roberta SantarosaLaboratório Nacional de Luz Síncrotron Jerry (Jerome) HastingsLinac Coherent Light Source Steven HulbertNational Synchrotron Light Source Thomas A RabedeauStanford Synchrotron Radiation Lightsource Mark BissenSynchrotron Radiation Center Gwyn WilliamsJefferson Lab Peter ReveszCornell High Energy Synchrotron Source Uwe ArpSynchrotron Ultraviolet Radiation Facility

  5. Challenges faced by research ethics committees in El Salvador: results from a focus group study.

    PubMed

    Camp, Jonathan W; Barfield, Raymond C; Rodriguez, Virginia; Young, Amanda J; Finerman, Ruthbeth; Caniza, Miguela A

    2009-04-01

    To identify perceived barriers to capacity building for local research ethics oversight in El Salvador, and to set an agenda for international collaborative capacity building. Focus groups were formed in El Salvador which included 17 local clinical investigators and members of newly formed research ethics committees. Information about the proposed research was presented to participants during an international bioethics colloquium sponsored and organized by the St. Jude Children's Research Hospital in collaboration with the National Ethics Committee of El Salvador and the University of El Salvador. Interviews with the focus group participants were qualitatively analyzed. Participants expressed the need to tailor the informed consent process and documentation to the local culture; for example, allowing family members to participate in decision-making, and employing shorter consent forms. Participants indicated that economic barriers often impede efforts in local capacity building. Participants valued international collaboration for mutual capacity building in research ethics oversight. Research ethics committees in El Salvador possess a basic knowledge of locally relevant ethical principles, though they need more training to optimize the application of bioethical principles and models to their particular contexts. Challenges increase the value of collaborative exchanges with ethics committee members in the United States. Further research on facilitating communication between host country and sponsor country ethics committees can maximize local research ethics expertise, and thus raise the standard of protecting human participants involved in international research.

  6. Cancer and leukemia group B clinical research associates committee.

    PubMed

    Price, Kandie C; Barrett, Barbara K; Roark, Jean M

    2006-06-01

    The Cancer and Leukemia Group B is dedicated to developing and implementing training programs that will enhance the skills and abilities of Clinical Research Associates (CRA) involved in all aspects of data collection and research. Training programs not only improve overall knowledge and professionalism but also improve the integrity of study data. The CRA roles and responsibilities include the following: collect, analyze, and monitor data; collaborate with other members of the health care team; insure regulatory mandates are followed; manage the care of research participants; assist in the recruitment and enrollment of human subjects; protect subjects rights by adhering to Institutional Review Board guidelines, the Code of Federal Regulations; prepare and submit timely adverse event experience reports; maintain case report forms and drug accountability records; educate other health care professionals, patients, and families regarding clinical trials; participate in research audits; and function as a team member with the research team. In addition, CRAs may also be responsible for writing reports, grant, and budget development and the development of protocols, forms, and informed consent documents.

  7. 77 FR 3030 - Membership in the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ...By Federal Register notice (See 76 FR 65319; October 20, 2011) the National Park Service (NPS) and the Federal Aviation Administration (FAA) invited interested persons to apply to fill a vacant position on the National Parks Overflights Advisory Group (NPOAG) Aviation Rulemaking Committee (ARC). That previous notice invited interested persons to apply to fill the vacancy representing Native......

  8. 77 FR 26068 - Notice of Meeting of the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-02

    ... National Parks Overflights Advisory Group Aviation Rulemaking Committee ACTION: Notice of meeting. SUMMARY: The Federal Aviation Administration (FAA) and the National Park Service (NPS), in accordance with the National Parks Air Tour Management Act of 2000, announce the next meeting of the National Parks...

  9. 78 FR 55336 - Membership in the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-10

    ... National Parks Overflights Advisory Group (NPOAG) Aviation Rulemaking Committee (ARC). The notice invited... Administration, Western-Pacific Region Headquarters, P.O. Box 92007, Los Angeles, CA 90009-2007, telephone: (310....'' Membership The current NPOAG ARC is made up of one member representing general aviation, three...

  10. 78 FR 52818 - Notice of Meeting of the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-26

    ... the National Parks Overflights Advisory Group (NPOAG) Aviation Rulemaking Committee (ARC). This notification provides the date, format, and agenda for the meeting. Date and Location: The NPOAG ARC will hold... Aviation Administration, Western-Pacific Region Headquarters, P.O. Box 92007, Los Angeles, CA...

  11. 75 FR 66828 - Eleventh Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-29

    ... for Air Traffic Data Communication Services AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data Communication... Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services. DATES: The...

  12. 76 FR 17473 - Twelfth Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-29

    ... for Air Traffic Data Communication Services AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data Communication... Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services. DATES: The meeting...

  13. The cancer and leukemia group B oncology nursing committee (1983-2006): a history of passion, commitment, challenge, and accomplishment.

    PubMed

    Smith, Ellen Lavoie; Skosey, Consuelo; Armer, Jane; Berg, Deborah; Cirrincione, Constance; Henggeler, Mary

    2006-06-01

    The Cancer and Leukemia Group B (CALGB) Oncology Nursing Committee (ONC) was initially established in 1983 as a working group with the specific aim of promoting protocol compliance through collaboration, communication, and education to enhance the scientific goals of the Group. Due to the efforts of its members, the committee gained full committee status. ONC members now serve as principal investigators and coinvestigators on research studies, continue to sponsor biannual educational sessions individually and in concert with other CALGB committees, and continue to develop tools to enlighten patients about their disease and the clinical trial process. The ONC, an administrative group of 12 members, provides leadership within CALGB. Although ONC members have always acted as liaisons to the disease and modality committees, three positions have recently been designated specifically for doctorally prepared nurse scientists. Since its inception, general nurse membership within the group has more than doubled to a total of more than 500 members.

  14. Report of the Task Group Calculations to ICRP Committee 2, October 14-17, 1980

    SciTech Connect

    Ford, M.R.

    1981-02-01

    This is the third in the series of reports of the Task Group on Dose Calculations to the International Commission on Radiological Protection (ICRP) Committee 2 as constituted for the four-year period 1977-1981. The two previous reports are documented in ORBL/TM-6988 (April 1979) and ORNL/TM-7230 (March 1980), respectively. Since the last meeting of the Committee, the Task Group has completed the computation of annual limit on intake (ALI) and derived air concentration (DAC) values and the corresponding supplemental data for 37 of the 43 elements planned for inclusion in the third and last part of the Committee report. The report entitled Limits for Intakes of Radionuclides by Workers, ICRP Publication 30 is being issued in three parts; and for each part, a supplement will be issued containing photocopied, computer-formatted supplemental data tables. Part 1 and the Supplement to Part 1 were both published in 1979, and the material for Part 2 and the Supplement to Part 2 are at Pergamon Press. Part 3 and Supplement will be ready for publication in 1981. The Task group plans to issue a separate report on radionuclide transformations used in estimating the ALI and DAC values in Publication 30. Preliminary data are being generated for this report, which will be timed to appear soon after the publication of Part 3 and the Supplement to Part 3.

  15. Committees

    NASA Astrophysics Data System (ADS)

    2003-08-01

    Born in Buenos Aires, Argentina, in 1930, Alberto Sirlin studied at the University of Buenos Aires from 1948-52, where he carried out research work in classical nonlinear physics, under the guidance of Richard Gans, and in 1953 received the degree of doctor in Physical-Mathematical Sciences. In 1953 he held a fellowship at the Centro Brasileiro de Pesquisas Fisicas in Rio de Janeiro, Brazil, where he did research work and attended some graduate courses, including a memorable and highly influential one taught by Richard Feynman. He spent the academic year 1954-55 at UCLA, where he initiated his work on electroweak physics in collaboration with Robert Finkelstein and Ralph Behrends. His next move was to Cornell University in 1955, earning his PhD there in 1958 for research in electroweak physics in collaboration with Toichiro Kinoshita. Sirlin spent 1957-59 as a research associate at Columbia University, becoming an Assistant Professor of Physics at New York University in 1959, an Associate Professor in 1961, and a full Professor in 1968. It is a remarkable coincidence that one of his fellow post-docs, Steven Weinberg, was to become one of the founders of the Standard Model, which in turn has provided the natural setting for Sirlin's work. During his formative years, he was extremely fortunate to receive guidance and advice from an extraordinary group of distinguished scientists, including R Gans, R P Feynman, R J Finkelstein, H A Bethe and E E Salpeter. He also enjoyed close and fruitful long-term collaborations with a number of brilliant theorists, including R E Behrends, T Kinoshita, T D Lee, M A B Beg, W J Marciano, P Langacker, G Degrassi, P Gambino and B A Kniehl, and has had fourteen excellent, interesting, and highly enterprising graduate students, who have remained close friends. Sirlin's main research interests have been in precision electroweak physics, other topics in weak interaction theory, the search for higher symmetries of the strong interactions

  16. 76 FR 62804 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work... outreach efforts. Entergy Regional State Committee Work Group and Stakeholder Meeting October 19, 2011 (9 a...

  17. 41 CFR 102-3.40 - What types of committees or groups are not covered by the Act and this part?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Established Within the Executive Branch? § 102-3.40 What types of committees or groups are not covered by the...) Committees not actually managed or controlled by the executive branch. Any committee or group created by non... are not actually managed or controlled by the executive branch; (e) Groups assembled to...

  18. Phase I Recommendations by the Air Quality Management Work Group to the Clean Air Act Advisory Committee

    EPA Pesticide Factsheets

    Recommendations to the Clean Air Act Advisory Committee by Air Quality Management Work Group addressing the recommendations made by the National Research Council to improve the U.S. air quality management system.

  19. Addendum to Air Quality: Decision Support Tools, Partner Plans, Working Groups, Committees

    NASA Technical Reports Server (NTRS)

    Holekamp, Kara; Frisbie, Troy; Estep, Lee

    2005-01-01

    In the original report dated February 11, 2005, the utility of the NASA Earth science data in the air quality activities of other agencies and organizations was assessed by reviewing strategic and mission plans and by conducting personal interviews with agency experts to identify and investigate agencies with the potential for partnership with NASA. The overarching agency strategic plans were reviewed and commonalities such as the desire for partnerships and technology development were noted. The addendum to the original report contains such information about the Tennessee Valley Authority and will be inserted in Section 2.6 of "Air Quality Decision Support Tools, Partner Plans, Working Groups, Committees".

  20. Addendum to Air Quality: Decision Support Tools, Partner Plans, Working Groups, Committees

    NASA Technical Reports Server (NTRS)

    Holekamp, Kara; Frisbie, Troy; Estep, Lee

    2005-01-01

    In the original report dated February 11, 2005, the utility of NASA Earth science data in the air quality activities of other agencies and organizations was assessed by reviewing strategic and mission plans and by conducting personal interviews with agency experts to identify and investigate agencies with the potential for partnership with NASA. The overarching agency strategic plans were reviewed and commonalities such as the desire for partnerships and technology development were noted. This addendum to the original report contains such information about the Tennessee Valley Authority and will be inserted as Section 2.6 of "Air Quality: Decision Support Tools, Partner Plans, Working Groups, Committees."

  1. Fifty years of clinical research by the leukemia committee of the cancer and leukemia group B.

    PubMed

    Larson, Richard A; Stone, Richard M; Mayer, Robert J; Schiffer, Charles A

    2006-06-01

    Progress in the care of patients with leukemia has been one of the great success stories in the field of oncology, and clinical research in leukemia has been the "flagship" of the Cancer and Leukemia Group B since the inception of this organization. Lessons learned from the founders' emphasis on childhood and adult leukemia have been extended broadly over the past 50 years to virtually all types of malignant diseases, and the Leukemia Committee has continued to provide leadership and key contributions. The Leukemia Committee is focused on the individualization of treatment based on distinctive biological and clinical characteristics with the aim of increasing efficacy and decreasing nonspecific toxicity. Our clinical trials in leukemia and myeloma have shifted from primarily empirically derived comparisons of different chemotherapeutic regimens to testing novel concepts such as the role of dose intensity, inhibition of specific mechanisms of drug resistance, the use of hematopoietic growth factors and monoclonal antibodies, and the utility of targeted agents. The Cancer and Leukemia Group B was the pioneer among the cooperative groups in the creation of centralized tissue repositories and the incorporation of correlative laboratory studies as an integral feature of clinical trials, a practice now termed "translational research." Considerable effort has focused on the identification of important pretreatment characteristics, such as morphologic features, immunophenotype, chromosomal abnormalities, and molecular defects, which are significantly associated with outcome in multivariable analyses and which enhance our understanding for the complex biology of these diseases.

  2. International consensus for neuroblastoma molecular diagnostics: report from the International Neuroblastoma Risk Group (INRG) Biology Committee.

    PubMed

    Ambros, P F; Ambros, I M; Brodeur, G M; Haber, M; Khan, J; Nakagawara, A; Schleiermacher, G; Speleman, F; Spitz, R; London, W B; Cohn, S L; Pearson, A D J; Maris, J M

    2009-05-05

    Neuroblastoma serves as a paradigm for utilising tumour genomic data for determining patient prognosis and treatment allocation. However, before the establishment of the International Neuroblastoma Risk Group (INRG) Task Force in 2004, international consensus on markers, methodology, and data interpretation did not exist, compromising the reliability of decisive genetic markers and inhibiting translational research efforts. The objectives of the INRG Biology Committee were to identify highly prognostic genetic aberrations to be included in the new INRG risk classification schema and to develop precise definitions, decisive biomarkers, and technique standardisation. The review of the INRG database (n=8800 patients) by the INRG Task Force finally enabled the identification of the most significant neuroblastoma biomarkers. In addition, the Biology Committee compared the standard operating procedures of different cooperative groups to arrive at international consensus for methodology, nomenclature, and future directions. Consensus was reached to include MYCN status, 11q23 allelic status, and ploidy in the INRG classification system on the basis of an evidence-based review of the INRG database. Standardised operating procedures for analysing these genetic factors were adopted, and criteria for proper nomenclature were developed. Neuroblastoma treatment planning is highly dependant on tumour cell genomic features, and it is likely that a comprehensive panel of DNA-based biomarkers will be used in future risk assignment algorithms applying genome-wide techniques. Consensus on methodology and interpretation is essential for uniform INRG classification and will greatly facilitate international and cooperative clinical and translational research studies.

  3. Small group processes relevant to data monitoring committees in controlled clinical trials: an overview of reviews.

    PubMed

    Walker, Anne E; McLeer, Sharon K

    2004-01-01

    The quality of the decisions reached by data monitoring committees (DMCs) is crucial. The aim of this paper is to identify factors that may make errors more or less likely in small, task-oriented, decision-making expert groups and to consider the implications of these factors for data monitoring committees. A systematic overview was carried out of reviews of empirical studies of small group processes and decision errors in small, task-oriented decision-making groups in laboratory or real-world settings, published between 1950 and 2002 (n = 57 included reviews). These reviews suggest that a number of factors may increase the likelihood that small groups will make poor and potentially erroneous decisions. The most important of these, in terms of empirical support, are: biased or overly directive leadership, expression of a limited range of opinions during group discussion, poor procedures for identifying or appraising the available information, and presentation of the available information in a way that is likely to result in biased perception of it. The main implications for DMCs relate to membership, the role of the chairperson, the information provided for DMCs and training for DMC members. Selection methods that encourage a degree of diversity within the DMC are recommended. Chairs of DMCs should be experienced members, who have the skills to facilitate a discussion, can manage conflict effectively and can be impartial. Adherence to a prespecified analysis plan is recommended to reduce the risk of error associated with strong evidence or excess information. Training in the use of methodical decision-making procedures, education about the factors that influence decision quality and an opportunity to participate in mock DMC discussions may be of benefit for new members.

  4. Report on the BWR owners group radiation protection/ALARA Committee

    SciTech Connect

    Aldrich, L.R.

    1995-03-01

    Radiation protection programs at U.S. boiling water reactor (BWR) stations have evolved during the 1980s and early 1990s from a regulatory adherence-based endeavor to a proactive, risk-based radiation protection and prevention mission. The objectives are no longer to merely monitor and document exposure to radiation and radioactive materials. The focus of the current programs is the optimization of radiation protection of occupational workers consistent with the purpose of producing cost-effective electric power. The newly revised 10 CFR 20 defines the term ALARA (as low as reasonably achievable) to take into account the state of technology, the economics of improvements in relation to the state of the technology, and the benefits to the public health and safety. The BWR Owners Group (BWROG) initially formed the Radiation Protection/ALARA Committee in January 1990 to evaluate methods of reducing occupational radiation exposure during refueling outages. Currently, twenty U.S. BWR owner/operators (representing 36 of the operational 37 domestic BWR units), as well as three foreign BWR operators (associate members), have broadened the scope to promote information exchange between BWR radiation protection professionals and develop good practices which will affect optimization of their radiation protection programs. In search of excellence and the challenge of becoming {open_quotes}World Class{close_quotes} performers in radiation protection, the BWROG Radiation Protection/ALARA Committee has recently accepted a role in assisting the member utilities in improving radiation protection performance in a cost-effective manner. This paper will summarize the recent activities of this Committee undertaken to execute their role of exchanging information in pursuit of optimizing the improvement of their collective radiation protection performance.

  5. Committee Effectiveness in Higher Education: The Strengths and Weaknesses of Group Decision Making

    ERIC Educational Resources Information Center

    Bates, Stephen B.

    2014-01-01

    Focusing on five models of committee effectiveness for purposes of this assessment will lend insight into the strengths and weaknesses of utilizing a structured action plan as a guide to achieving and maintaining optimum committee effectiveness in higher education. In the compilation of the strengths and weaknesses of committee decision making,…

  6. Report from the Radiation Therapy Committee of the Southwest Oncology Group (SWOG): Research Objectives Workshop 2008

    PubMed Central

    Okunieff, Paul; Kachnic, Lisa A; Constine, Louis S; Fuller, Clifton D; Gaspar, Laurie E; Hayes, Daniel F; Hooks, Jean; Ling, Clifton; Meyskens, Frank L; Philip, Philip A; Raben, David; Smalley, Stephen R; Swanson, Gregory P; Teicher, Beverly A; Thomas, Charles R; Vikram, Bhadrasain; Zelefsky, Michael J; Baker, Laurence H

    2010-01-01

    Strategic planning for the Radiation Therapy Committee of the Southwest Oncology Group (SWOG) is comprehensively evaluated every six years in an effort to maintain a current and relevant scientific focus, and to provide a standard platform for future development of protocol concepts. Participants in the 2008 Strategic Planning Workshop included clinical trial experts from multiple specialties, industry representatives from both pharmaceuticals and equipment manufacturers, and basic scientists. High priority research areas such as image-guided radiation therapy for control of limited metastatic disease, analysis of biomarkers for treatment response and late toxicity, assessment of novel agents in combination with radiation, standardization of radiation target delineation, and the assessment of new imaging techniques to individualize cancer therapy, were discussed. Research priorities included clinical study designs featuring translational endpoints that identify patients most likely to benefit from combined modality therapy; intervention including combination radiation with standard chemotherapy; radiation with radiosensitizing molecular-targeted therapies; and stereotactic radiation for treatment of patients with regard to asymptomatic metastasis and radiation-induced tumor autoimmunity. The Committee concluded that the future research opportunities are among the most exciting to have developed in the last decade, and work is in progress to embark on these plans. PMID:19723641

  7. Measurement and Basic Physics Committee of the U.S. Cross-Section Evaluation Working Group annual report 1997

    SciTech Connect

    Smith, D.L.; McLane, V.

    1997-10-01

    The Cross-Section Evaluation Working Group (CSEWG) is a long-standing committee charged with responsibility for organizing and overseeing the US cross-section evaluation effort. It`s main product is the official US evaluated nuclear data file, ENDF. In 1992 CSEWG added the Measurements Committee to its list of standing committees and subcommittees. This action was based on a recognition of the importance of experimental data in the evaluation process as well as the realization that measurement activities in the US were declining at an alarming rate and needed considerable encouragement to avoid the loss of this resource. The mission of the Committee is to maintain contact with experimentalists in the Us and to encourage them to contribute to the national nuclear data effort. Improved communication and the facilitation of collaborative activities are among the tools employed in achieving this objective. In 1994 the Committee was given an additional mission, namely, to serve as an interface between the applied interests represented in CSEWG and the basic nuclear science community. Accordingly, its name was changed to the Measurement and Basic Physics Committee. The present annual report is the third such document issued by the Committee. It contains voluntary contributions from several laboratories in the US. Their contributions were submitted to the Chairman for compilation and editing.

  8. Activitivities of Japanese EMTP Committee (JEC) and EMTP Development Coordination Group Project (EMTP-DCG Project)

    NASA Astrophysics Data System (ADS)

    Nagaoka, Naoto; Motoyama, Hideki; Funabashi, Toshihisa

    The Electromagnetic Transients Program (EMTP) is a common software for analyzing the electromagnetic transient phenomena, the electromechanical dynamics, and the control system transients in electric power systems and electronic circuits. Since the late 1960's, the EMTP has been developed by many contributors, and various capabilities, functions and auxiliary programs have been added to the EMTP. In the first half of the paper, history of Japanese EMTP Committee (JEC) and its activity is described. The basic policy of the JEC is “neutral” and “public domain”. The objectives, members, recent workshops and publications are introduced in this report. The activity of the JEC covers any EMTP version, such as ATP- and DCG-EMTP, derived from the BPA-EMTP. The second part of this paper presents the history and products of the EMTP Development Coordination Group Project (EMTP-DCG Project), which is organized to rationalize the development of the EMTP.

  9. Brain Malignancy Steering Committee clinical trials planning workshop: report from the Targeted Therapies Working Group.

    PubMed

    Alexander, Brian M; Galanis, Evanthia; Yung, W K Alfred; Ballman, Karla V; Boyett, James M; Cloughesy, Timothy F; Degroot, John F; Huse, Jason T; Mann, Bhupinder; Mason, Warren; Mellinghoff, Ingo K; Mikkelsen, Tom; Mischel, Paul S; O'Neill, Brian P; Prados, Michael D; Sarkaria, Jann N; Tawab-Amiri, Abdul; Trippa, Lorenzo; Ye, Xiaobu; Ligon, Keith L; Berry, Donald A; Wen, Patrick Y

    2015-02-01

    Glioblastoma is the most common primary brain malignancy and is associated with poor prognosis despite aggressive local and systemic therapy, which is related to a paucity of viable treatment options in both the newly diagnosed and recurrent settings. Even so, the rapidly increasing number of targeted therapies being evaluated in oncology clinical trials offers hope for the future. Given the broad range of possibilities for future trials, the Brain Malignancy Steering Committee convened a clinical trials planning meeting that was held at the Udvar-Hazy Center in Chantilly, Virginia, on September 19 and 20, 2013. This manuscript reports the deliberations leading up to the event from the Targeted Therapies Working Group and the results of the meeting.

  10. COMMITTEES COMMITTEES

    NASA Astrophysics Data System (ADS)

    2010-11-01

    ORGANISING COMMITTEE Chairman: Alexander G Petrov Director, Institute of Solid State Physics, Bulgarian Academy of Sciences, Sofia, Bulgaria Chairman Emeritus: Nikolay Kirov Institute of Solid State Physics, Bulgarian Academy of Sciences, Sofia, Bulgaria Local Organising Committee: Chairman: Alexander G Petrov Members: Diana Nesheva, Doriana Dimova-Malinovska, Eleonora Popova, Lyubomila Dedinska, Christo Popov, Vasil Lovchinov, Marina Primatarowa, Emilia Vlaikova, Irina Velkova, Hassan Chamati PROGRAMME COMMITTEE Chairman: A G Petrov Members: D Alexandrov (Thunder Bay), V Celebonovic (Belgrade), D Dimova-Malinovska (Sofia), B Dulmet (Besancon), A Grechnikov (Moscow), M Gunes (Mugla), C Main (Dundee), D Nesheva (Sofia), M C Petty (Durham), M Popescu (Bucharest), S Reynolds (Dundee), K Shimakawa (Gifu), J Singh (Darwin), N Starbov (Sofia), M Tomilin (St Petersburg), Ph Vanderbemden (Liege), A Vaseashta (Washington) LOCAL SCIENTIFIC COUNCIL Chairman: A G Petrov Members: Bulgarian Academy of Sciences N Sabotinov (President) I Nedkov (Scientific Secretary, Physics) Institute of Solid State Physics N Kirov, D Nesheva, V Lovchinov, St Andreev, M Primatarowa Institute of Electronics R Enikov (Director) Central Lab. of Solar Energy and New Energy Sources D Dimova-Malinovska Institute of Optical Materials and Technologies V Saynov, N Starbov Central Lab. of Applied Physics R Kakanakov (Director) Sofia University - Faculty of Physics A Andreeva, S Russev (Heads of Departments)

  11. COMMITTEES: Committees

    NASA Astrophysics Data System (ADS)

    2010-01-01

    TAUP STEERING COMMITTEE F T Avignone, University of South Carolina B C Barish, CALTECH E Bellotti, University of Milano, INFN J Bernabeu, University of Valencia A Bottino (Chair), University of Torino, INFN N Fornengo, University of Torino, INFN T Kajita, ICRR University of Tokyo C W Kim, Johns Hopkins University, KIAS V Matveev, INR Moscow J Morales, University of Zaragoza G Raffelt, MPI Munchen D Sinclair, University of Carleton M Spiro, IN2P3 TAUP 2009 INTERNATIONAL ADVISORY COMMITTEE J J Aubert, CNRS Marseille M Baldo-Ceolin, University of Padova, INFN G Bellini, University of Milano, INFN L Bergstrom, University of Stockholm R Bernabei, University of Roma Tor Vergata, INFN A Bettini, University of Padova, INFN, LSC S Bilenky, JINR Dubna D O Caldwell, UCSB J Cronin, University of Chicago A Dar, Technion Haifa G Domogatsky, INR Moscow J Ellis, CERN E Fernandez, IFAE Barcelona E Fiorini, University of Milano, INFN T Gaisser, University of Delaware G Gelmini, UCLA G Gerbier, CEA Saclay A Giazotto, INFN Pisa F Halzen, University of Wisconsin W Haxton, University of Washington T Kirsten MPI Heidelberg L Maiani, University of Roma La Sapienza, INFN A McDonald, Queen's University K Nakamura, KEK R Petronzio, INFN, University of Roma Tor Vergata L Resvanis, University of Athens F Ronga INFN, LNF C Rubbia INFN, LNGS A Smirnov, ICTP Trieste C Spiering, DESY N Spooner, University of Sheffield A Suzuki, KEK S Ting MIT, CERN M S Turner, FNAL, University of Chicago J W F Valle, IFIC Valencia D Vignaud, APC Paris G Zatsepin, INR Moscow TAUP 2009 ORGANIZING COMMITTEE R Aloisio, LNGS R Antolini, LNGS F Arneodo, LNGS Z Berezhiani, University of L'Aquila, INFN V Berezinsky, LNGS R Cerulli, LNGS E Coccia [Chair], LNGS/INFN, U of Roma Tor Vergata N D'Ambrosio, LNGS N Fornengo, University of Torino, INFN M Laubenstein, LNGS O Palamara, LNGS L Pandola [Scientific Secretary], LNGS

  12. How do small groups make decisions? : A theoretical framework to inform the implementation and study of clinical competency committees.

    PubMed

    Chahine, Saad; Cristancho, Sayra; Padgett, Jessica; Lingard, Lorelei

    2017-06-01

    In the competency-based medical education (CBME) approach, clinical competency committees are responsible for making decisions about trainees' competence. However, we currently lack a theoretical model for group decision-making to inform this emerging assessment phenomenon. This paper proposes an organizing framework to study and guide the decision-making processes of clinical competency committees.This is an explanatory, non-exhaustive review, tailored to identify relevant theoretical and evidence-based papers related to small group decision-making. The search was conducted using Google Scholar, Web of Science, MEDLINE, ERIC, and PsycINFO for relevant literature. Using a thematic analysis, two researchers (SC & JP) met four times between April-June 2016 to consolidate the literature included in this review.Three theoretical orientations towards group decision-making emerged from the review: schema, constructivist, and social influence. Schema orientations focus on how groups use algorithms for decision-making. Constructivist orientations focus on how groups construct their shared understanding. Social influence orientations focus on how individual members influence the group's perspective on a decision. Moderators of decision-making relevant to all orientations include: guidelines, stressors, authority, and leadership.Clinical competency committees are the mechanisms by which groups of clinicians will be in charge of interpreting multiple assessment data points and coming to a shared decision about trainee competence. The way in which these committees make decisions can have huge implications for trainee progression and, ultimately, patient care. Therefore, there is a pressing need to build the science of how such group decision-making works in practice. This synthesis suggests a preliminary organizing framework that can be used in the implementation and study of clinical competency committees.

  13. C12A7 Electride Hollow Cathode

    DTIC Science & Technology

    2013-03-01

    those found in clathrate phases of ice and in zeolites , there is an important difference in that the unit cell of C12A7 is positively charged. In other...while Ba-W is heated above 1300 K (Goebel, Watkins & Jameson, 2007). These temperatures require well-made heaters and good thermal insulation. Ba-W...Chu, L. (2006, July 9-12). Characterization of Hollow Cathode Performance and Thermal Behavior. AIAA-2006-5150. Sacramento, California. 11

  14. 76 FR 67727 - Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Notice of FERC Staff Attendance at the Entergy Regional State Committee Work Group and Stakeholder Meeting The Federal Energy Regulatory Commission hereby gives notice that members...

  15. Joint Funding Councils' Libraries Review Group (the "Follett") Report: The Contribution of the Information Technology Sub-Committee

    ERIC Educational Resources Information Center

    Brindley, Lynne J.

    2006-01-01

    Purpose: This article aims to look into a review of library provision in higher education, which was originally set up in 1992 by The Funding Councils of England, Scotland and Wales, and the Department of Education for Northern Ireland. Design/methodology/approach: The review group was structured into three sub-committees, under the umbrella of…

  16. 77 FR 5031 - Notice of a Meeting of a Working Group of the Advisory Committee to the Director

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-01

    ... HUMAN SERVICES National Institutes of Health Notice of a Meeting of a Working Group of the Advisory Committee to the Director SUMMARY: The purpose of this notice is to inform the public about a meeting of the... (WGBDRW). DATES: The meeting will be held on Tuesday, February 14, 2012, from approximately 10 a.m. to 3...

  17. 75 FR 22674 - U.S. Department of State Advisory Committee on Private International Law Study Group Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-29

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF STATE U.S. Department of State Advisory Committee on Private International Law Study Group Notice of Meeting on the United Nations Commission on International Trade Law (UNCITRAL) Draft Legislative Guide on...

  18. 75 FR 32834 - U.S. Department of State Advisory Committee on Private International Law Study Group Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-09

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE U.S. Department of State Advisory Committee on Private International Law Study Group Notice of Meeting on the United Nations Commission on International Trade Law (UNCITRAL) Draft Legislative Guide on...

  19. MEASUREMENT AND BASIC PHYSICS COMMITTEE OF THE U.S. CROSS-SECTION EVALUATION WORKING GROUP, ANNUAL REPORT 1997

    SciTech Connect

    SMITH,D.L.; MCLANE,V.

    1998-10-20

    The Cross-Section Evaluation Working Group (CSEWG) is a long-standing committee charged with responsibility for organizing and overseeing the US cross-section evaluation effort. Its main product is the official US evaluated nuclear data file, ENDF. The current version of this file is Version VI. All evaluations included in ENDF, as well as periodic modifications and updates to the file, are reviewed and approved by CSEWG and issued by the US Nuclear Data Center, Brookhaven National Laboratory. CSEWG is comprised of volunteers from the US nuclear data community who possess expertise in evaluation methodologies and who collectively have been responsible for producing most of the evaluations included in ENDF. In 1992 CSEWG added the Measurements Committee to its list of standing committees and subcommittees. This action was based on a recognition of the importance of experimental data in the evaluation process as well as the realization that measurement activities in the US were declining at an alarming rate and needed considerable encouragement to avoid the loss of this resource. The mission of the Committee is to maintain contact with experimentalists in the US and to encourage them to contribute to the national nuclear data effort. Improved communication and the facilitation of collaborative activities are among the tools employed in achieving this objective. In 1994 the Committee was given an additional mission, namely, to serve as an interface between the applied interests represented in CSEWG and the basic nuclear science community. Accordingly, its name was changed to the Measurement and Basic Physics Committee. The present annual report is the third such document issued by the Committee. It contains voluntary contributions from several laboratories in the US. Their contributions were submitted to the Chairman for compilation and editing.

  20. Trip report, Interagency Manufacturing Operations Group (IMOG) Steering Committee meeting, November 29, 1989

    SciTech Connect

    Butterworth, R.R.

    1989-12-06

    As the Subgroup Chairman for the Process Automation Control Technology Subgroup of IMOG, I was requested to attend the annual Steering Committee Meeting held this year at the Allied-Signal Kansas City Plant and summarize the past year's activities of the Subgroup. The next IMOG Steering Committee Meeting will be held November 14 and 15, 1990 in Los Alamos. The next Process Automation Control Technology Subgroup Meeting will be held in June, 1990 in Rocky Flats.

  1. 76 FR 10085 - Membership in the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-23

    ...) Aviation Rulemaking Committee (ARC). These notices invited interested persons to apply to fill two... vacancies on the NPOAG ARC. FOR FURTHER INFORMATION CONTACT: Barry Brayer, Special Programs Staff, Federal Aviation Administration, Western-Pacific Region Headquarters, P.O. Box 92007, Los Angeles, CA...

  2. 75 FR 69154 - Notice of Meeting of the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-10

    ... Rulemaking Committee (ARC). This notification provides the dates, location, and agenda for the meeting. Dates and Location: The NPOAG ARC will meet on November 30, 2010 and December 1, 2010. The meeting will take... Region Headquarters, P.O. Box 92007, Los ] Angeles, CA 90009-2007, telephone: (310) 725-3800,...

  3. Veterinary Products Committee working group report on feline and canine vaccination.

    PubMed

    Gaskell, R M; Gettinby, G; Graham, S J; Skilton, D

    2002-02-02

    * The working group was set up by the Veterinary Products Committee in response to current concern in both the public domain and in the scientific community about possible health risks related to the routine vaccination of cats and dogs. The working group concluded that vaccination plays a very valuable role in the prevention and control of the major infectious diseases in cats and dogs. Although adverse reactions to vaccination, including lack of efficacy, occasionally occur, the working group concluded that the overall risk/benefit analysis strongly supports their continued use. * Although for some diseases there is evidence of a longer duration of immunity following vaccination than the one year which is typically recommended on the product literature, there is currently insufficient information to propose revaccination intervals other than those proposed by the manufacturer and approved by the regulatory process. * Notwithstanding this, in view of the occasional occurrence of adverse reactions, the working group recommends that the product literature indicates that the regime for booster vaccinations is based on a minimum duration of immunity rather than a maximum. The working group further recommends that the product literature should state that a risk/benefit assessment should be made for each individual animal by the veterinary surgeon in consultation with the owner with respect to the necessity for each vaccine and the frequency of its use. * The evidence suggests that cats appear to be susceptible to the occasional development of sarcomas at sites of injection and there is some further evidence to suggest that, although other products may be involved, this may be more associated with the use of vaccines containing aluminium-based adjuvants. The working group therefore recommends that a generic warning to this effect should appear on the product literature for all feline vaccines administered by injection. The working group also highlighted the need for

  4. Compaction of DNA using C12EO4 cooperated with Fe(3.).

    PubMed

    Wang, Ling; Xu, Lu; Li, Guihua; Feng, Lei; Dong, Shuli; Hao, Jingcheng

    2016-08-01

    Nonionic surfactant, tetraethylene glycol monododecyl ether (C12EO4), cannot compact DNA because of its low efficiency in neutralizing the negative charges of the phosphate groups of DNA. It is also well-known that nonionic surfactants as a decompaction agent can help DNA be released from cationic surfactant aggregates. Herein, with the "bridge" Fe(3+) of C12EO4, we found that C12EO4 can efficiently compact DNA molecules into globular states with a narrow size distribution, indicating that the cooperative Fe(3+) can transform C12EO4 molecules from decompaction agents to compaction ones. The mechanism of the interaction of DNA and C12EO4 by "bridge" Fe(3+) is that the Fe(3+)-C12EO4 complexes act as multivalent ions by cooperative and hydrophobic interaction. The improved colloidal-stability and endosome escape effect induced by C12EO4 would provide the potential applications of nonionic surfactant in the physiological characteristics of DNA complexes. Cell viability assay demonstrates that Fe(3+)-C12EO4 complexes possess low cytotoxicity, ensuring good biocompatibility. Another advantage of this system is that the DNA complexes can be de-compacted by glutathione in cell without any other agents. This suggests the metal ion-nonionic surfactant complexes as compaction agent can act as the potential delivery tool of DNA in future nonviral gene delivery systems.

  5. Cancer and Leukemia Group B Pathology Committee guidelines for tissue microarray construction representing multicenter prospective clinical trial tissues.

    PubMed

    Rimm, David L; Nielsen, Torsten O; Jewell, Scott D; Rohrer, Daniel C; Broadwater, Gloria; Waldman, Frederic; Mitchell, Kisha A; Singh, Baljit; Tsongalis, Gregory J; Frankel, Wendy L; Magliocco, Anthony M; Lara, Jonathan F; Hsi, Eric D; Bleiweiss, Ira J; Badve, Sunil S; Chen, Beiyun; Ravdin, Peter M; Schilsky, Richard L; Thor, Ann; Berry, Donald A

    2011-06-01

    Practice-changing evidence requires confirmation, preferably in multi-institutional clinical trials. The collection of tissue within such trials has enabled biomarker studies and evaluation of companion diagnostic tests. Tissue microarrays (TMAs) have become a standard approach in many cooperative oncology groups. A principal goal is to maximize the number of assays with this precious tissue. However, production strategies for these arrays have not been standardized, possibly decreasing the value of the study. In this article, members of the Cancer and Leukemia Group B Pathology Committee relay our experiences as array facility directors and propose guidelines regarding the production of high-quality TMAs for cooperative group studies. We also discuss statistical issues arising from having a proportion of patients available for TMAs and the possibility that patients with TMAs fail to represent the greater study population.

  6. Ensuring Resident Competence: A Narrative Review of the Literature on Group Decision Making to Inform the Work of Clinical Competency Committees

    PubMed Central

    Hauer, Karen E.; Cate, Olle ten; Boscardin, Christy K.; Iobst, William; Holmboe, Eric S.; Chesluk, Benjamin; Baron, Robert B.; O'Sullivan, Patricia S.

    2016-01-01

    Background The expectation for graduate medical education programs to ensure that trainees are progressing toward competence for unsupervised practice prompted requirements for a committee to make decisions regarding residents' progress, termed a clinical competency committee (CCC). The literature on the composition of these committees and how they share information and render decisions can inform the work of CCCs by highlighting vulnerabilities and best practices. Objective We conducted a narrative review of the literature on group decision making that can help characterize the work of CCCs, including how they are populated and how they use information. Methods English language studies of group decision making in medical education, psychology, and organizational behavior were used. Results The results highlighted 2 major themes. Group member composition showcased the value placed on the complementarity of members' experience and lessons they had learned about performance review through their teaching and committee work. Group processes revealed strengths and limitations in groups' understanding of their work, leader role, and information-sharing procedures. Time pressure was a threat to the quality of group work. Conclusions Implications of the findings include the risks for committees that arise with homogeneous membership, limitations to available resident performance information, and processes that arise through experience rather than deriving from a well-articulated purpose of their work. Recommendations are presented to maximize the effectiveness of CCC processes, including their membership and access to, and interpretation of, information to yield evidence-based, well-reasoned judgments. PMID:27168881

  7. 75 FR 18014 - Membership in the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-08

    ... Federal Aviation Administration Membership in the National Parks Overflights Advisory Group Aviation..., 2010) the National Park Service (NPS) and the Federal Aviation Administration (FAA) invited interested persons to apply to fill a vacant position on the National Parks Overflights Advisory Group...

  8. 75 FR 4904 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-29

    ... Group was established. The Working Group monitored the steam locomotive regulation task. Planned future... recommending revisions to Steam Locomotive Inspection Standards (49 CFR Part 230). Task 96-6--(Completed... Front-End Strength of Cab Cars and Multiple-Unit Locomotives NPRM was published in the Federal Register...

  9. 75 FR 22561 - Federal Advisory Committee; United States Strategic Command Strategic Advisory Group; Charter...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-29

    ... Nation's strategic forces, as requested by the Chairman of the Joint Chiefs of Staff or the Commander of... authorities in the fields of strategic policy formulation, nuclear weapon design and national command, control... forces. Group members shall be appointed by the Secretary of Defense, and their membership shall...

  10. 78 FR 25338 - Membership in the National Parks Overflights Advisory Group Aviation Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-30

    ... Federal Aviation Administration Membership in the National Parks Overflights Advisory Group Aviation... FR 5242-5243, January 24, 2013) the National Park Service (NPS) and the Federal Aviation Administration (FAA) invited interested persons to apply to fill one opening on the National Parks...

  11. Role of Advisory Groups. Instructor Guide. Working for Clean Water: An Information Program for Advisory Committees.

    ERIC Educational Resources Information Center

    Pennsylvania State Univ., Middletown. Inst. of State and Regional Affairs.

    Presented is an instructor's manual for conducting a learning session about public participation in water quality planning. Participants in the session learn about the purpose, organization, and activities of citizen advisory groups. The manual, a component of the Working for Clean Water Project, is designed for use in conjunction with a…

  12. 77 FR 24257 - Railroad Safety Advisory Committee (RSAC); Working Group Activity Update

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-23

    ... braking system requirements and international braking system requirements versus existing U.S... consider use of service proven braking systems. The Task Group will also consider performance-based... approved by the full RSAC on June 26, 2007. The Passenger Train Emergency Systems final rule, focusing...

  13. Facile controlled synthesis of micro/nanostructure MCrO 4 (M = Ba, Pb) by using Gemini surfactant C 12-PEG-C 12 as a soft template

    NASA Astrophysics Data System (ADS)

    Chang, Wengui; Shen, Yuhua; Xie, Anjian; Liu, Xue

    2010-04-01

    Gemini surfactants, double sodium α-sulfonic polyethylene glycol laurate (abbreviated C 12-PEG-C 12), were prepared and applied as soft templates in the controlled synthesis of BaCrO 4 and PbCrO 4 micro/nanocrystals. The template effects were investigated by adjusting the length of the spacer, using PEG400 and PEG4000, of the Gemini surfactant. The results indicated that the size and morphology of BaCrO 4 and PbCrO 4 micro/nanocrystals varied with the change in spacer length of C 12-PEG-C 12, suggesting that the different lengths of the polyethylene glycol group spacers in the Gemini surfactants played a key role in determining the size and shape of the MCrO 4 micro/nanoparticles. The dynamic process of the formation of the novel morphology BaCrO 4 crystals showed that the morphology grew from a round-bar polyhedron, to regular polyhedron, to approximate octahedron to a uniform pistachio nut shape. The growth mechanism of the BaCrO 4 micro/nanocrystals was explained that C 12-PEG-C 12 had a greater interfacial adsorption and would effectively control the shape evolution during the crystal growth, while PbCrO 4 could be explained that the Gemini surfactants can undergo liquid-crystalline phase transitions with long channels providing a soft template effect and derived the nanorods formation. Room temperature fluorescence spectra were studied and these showed that the pistachio-shaped BaCrO 4 microcrystals and PbCrO 4 nanorods possess photoactive luminescence properties with emission peaks at 470 and 549 nm, respectively.

  14. Reports and recommendations from COSPAR Planetary Exploration Committee (PEX) & International Lunar Exploration Working Group (ILEWG)

    NASA Astrophysics Data System (ADS)

    Ehrenfreund, Pascale; Foing, Bernard

    2014-05-01

    In response to the growing importance of space exploration, the objectives of the COSPAR Panel on Exploration (PEX) are to provide high quality, independent science input to support the development of a global space exploration program while working to safeguard the scientific assets of solar system bodies. PEX engages with COSPAR Commissions and Panels, science foundations, IAA, IAF, UN bodies, and IISL to support in particular national and international space exploration working groups and the new era of planetary exploration. COSPAR's input, as gathered by PEX, is intended to express the consensus view of the international scientific community and should ultimately provide a series of guidelines to support future space exploration activities and cooperative efforts, leading to outstanding scientific discoveries, opportunities for innovation, strategic partnerships, technology progression, and inspiration for people of all ages and cultures worldwide. We shall focus on the lunar exploration aspects, where the COSPAR PEX is building on previous COSPAR, ILEWG and community conferences. An updated COSPAR PEX report is published and available online (Ehrenfreund P. et al, COSPAR planetary exploration panel report, http://www.gwu.edu/~spi/assets/COSPAR_PEX2012.pdf). We celebrate 20 years after the 1st International Conference on Exploration and Utilisation of the Moon at Beatenberg in June 1994. The International Lunar Exploration Working Group (ILEWG) was established the year after in April 1995 at an EGS meeting in Hamburg, Germany. As established in its charter, this working group reports to COSPAR and is charged with developing an international strategy for the exploration of the Moon (http://sci.esa.int/ilewg/ ). It discusses coordination between missions, and a road map for future international lunar exploration and utilisation. It fosters information exchange or potential and real future lunar robotic and human missions, as well as for new scientific and

  15. American Association of Physicists in Medicine Radiation Therapy Committee Task Group 53: quality assurance for clinical radiotherapy treatment planning.

    PubMed

    Fraass, B; Doppke, K; Hunt, M; Kutcher, G; Starkschall, G; Stern, R; Van Dyke, J

    1998-10-01

    In recent years, the sophistication and complexity of clinical treatment planning and treatment planning systems has increased significantly, particularly including three-dimensional (3D) treatment planning systems, and the use of conformal treatment planning and delivery techniques. This has led to the need for a comprehensive set of quality assurance (QA) guidelines that can be applied to clinical treatment planning. This document is the report of Task Group 53 of the Radiation Therapy Committee of the American Association of Physicists in Medicine. The purpose of this report is to guide and assist the clinical medical physicist in developing and implementing a comprehensive but viable program of quality assurance for modern radiotherapy treatment planning. The scope of the QA needs for treatment planning is quite broad, encompassing image-based definition of patient anatomy, 3D beam descriptions for complex beams including multileaf collimator apertures, 3D dose calculation algorithms, and complex plan evaluation tools including dose volume histograms. The Task Group recommends an organizational framework for the task of creating a QA program which is individualized to the needs of each institution and addresses the issues of acceptance testing, commissioning the planning system and planning process, routine quality assurance, and ongoing QA of the planning process. This report, while not prescribing specific QA tests, provides the framework and guidance to allow radiation oncology physicists to design comprehensive and practical treatment planning QA programs for their clinics.

  16. Primary nephrectomy and intraoperative tumor spill: report from the Children's Oncology Group (COG) renal tumors committee.

    PubMed

    Gow, Kenneth W; Barnhart, Douglas C; Hamilton, Thomas E; Kandel, Jessica J; Chen, Mike K S; Ferrer, Fernando A; Price, Mitchell R; Mullen, Elizabeth A; Geller, James I; Gratias, Eric J; Rosen, Nancy; Khanna, Geetika; Naranjo, Arlene; Ritchey, Michael L; Grundy, Paul E; Dome, Jeffrey S; Ehrlich, Peter F

    2013-01-01

    Initial Children's Oncology Group (COG) management for Wilms' tumor (WT) consists of primary nephroureterectomy with lymph node sampling. While this provides accurate staging to define further treatment, it may result in intraoperative spill (IOS), which is associated with higher recurrence rates and therefore requires more intensive therapy. The purpose of this study is to determine current rates and identify factors which may predispose a patient to IOS. The study population was drawn from the AREN03B2 renal tumor banking and classification study of the Children's Oncology Group. All children with a first time occurrence of a renal mass were eligible for the study. At the time of enrollment and prior to risk stratification, the institution is required to submit operative notes, pathology specimens, a chest computed tomography scan (CT), and a contrast-enhanced CT or magnetic resonance imaging (MRI) of the abdomen and pelvis for central imaging review. These data are then used to determine an initial risk classification and therapeutic protocol eligibility. Patients who had a unilateral nephroureterectomy for favorable histology WT underwent further review to assure data accuracy and to clarify details regarding the spill. Analyses were performed using chi square and logistic regression. Odd ratios (OR) are shown with 95% confidence intervals. There were 1,131 primary nephrectomies for unilateral WT with an IOS rate of 9.7% with an additional 1.8% having possible tumor spill during renal vein or IVC tumor thrombectomy. IOS correlated with diameter (>12 cm, p<0.0001) and laterality (right, p=0.0414). Simple logistic regression indicated that IOS increased 2.7% [p=0.0240, OR 1.027 (1.004, 1.052)] with each 1 cm increase in diameter (3 - 21 cm) and 4.7% [p=0.0147 OR 1.047 (1.009, 1.086)] with each 100 g increase in weight (80 - 1800 g). Multiple logistic regression indicated that laterality [right p=0.048, OR 1.46 (1.004, 2.110)] and weight (p=0.03, OR 1.039 (1

  17. Ongoing quality control in digital radiography: Report of AAPM Imaging Physics Committee Task Group 151

    SciTech Connect

    Jones, A. Kyle Geiser, William; Heintz, Philip; Goldman, Lee; Jerjian, Khachig; Martin, Melissa; Peck, Donald; Pfeiffer, Douglas; Ranger, Nicole; Yorkston, John

    2015-11-15

    Quality control (QC) in medical imaging is an ongoing process and not just a series of infrequent evaluations of medical imaging equipment. The QC process involves designing and implementing a QC program, collecting and analyzing data, investigating results that are outside the acceptance levels for the QC program, and taking corrective action to bring these results back to an acceptable level. The QC process involves key personnel in the imaging department, including the radiologist, radiologic technologist, and the qualified medical physicist (QMP). The QMP performs detailed equipment evaluations and helps with oversight of the QC program, the radiologic technologist is responsible for the day-to-day operation of the QC program. The continued need for ongoing QC in digital radiography has been highlighted in the scientific literature. The charge of this task group was to recommend consistency tests designed to be performed by a medical physicist or a radiologic technologist under the direction of a medical physicist to identify problems with an imaging system that need further evaluation by a medical physicist, including a fault tree to define actions that need to be taken when certain fault conditions are identified. The focus of this final report is the ongoing QC process, including rejected image analysis, exposure analysis, and artifact identification. These QC tasks are vital for the optimal operation of a department performing digital radiography.

  18. Ongoing quality control in digital radiography: Report of AAPM Imaging Physics Committee Task Group 151.

    PubMed

    Jones, A Kyle; Heintz, Philip; Geiser, William; Goldman, Lee; Jerjian, Khachig; Martin, Melissa; Peck, Donald; Pfeiffer, Douglas; Ranger, Nicole; Yorkston, John

    2015-11-01

    Quality control (QC) in medical imaging is an ongoing process and not just a series of infrequent evaluations of medical imaging equipment. The QC process involves designing and implementing a QC program, collecting and analyzing data, investigating results that are outside the acceptance levels for the QC program, and taking corrective action to bring these results back to an acceptable level. The QC process involves key personnel in the imaging department, including the radiologist, radiologic technologist, and the qualified medical physicist (QMP). The QMP performs detailed equipment evaluations and helps with oversight of the QC program, the radiologic technologist is responsible for the day-to-day operation of the QC program. The continued need for ongoing QC in digital radiography has been highlighted in the scientific literature. The charge of this task group was to recommend consistency tests designed to be performed by a medical physicist or a radiologic technologist under the direction of a medical physicist to identify problems with an imaging system that need further evaluation by a medical physicist, including a fault tree to define actions that need to be taken when certain fault conditions are identified. The focus of this final report is the ongoing QC process, including rejected image analysis, exposure analysis, and artifact identification. These QC tasks are vital for the optimal operation of a department performing digital radiography.

  19. The C-12/C-13 Ratio as a Chemistry Indicator

    NASA Technical Reports Server (NTRS)

    Wirstroem, Eva; Geppert, Wolf; Persson, Carina; Charnley, Steven

    2011-01-01

    Isotopic ratios of elements are considered powerful tools, e.g. in tracing the origin of solar system body materials, or the degree of nucleosynthesis processing throughout the Galaxy. In interstellar molecules, some isotopic ratios like H/D and C-12/C-13 can also be used as indicators of their chemical origin. Isotope fractionation in gas-phase chemical reactions and gas-dust interaction makes observations of the ratio between C-12 and C-13 isotopologues suitable to distinguish between different formation scenarios. We will present observations of the C-12/C-13 ratio in methanol and formaldehyde towards a sample of embedded, massive young stellar objects. In relation to this we also present results from theoretical modeling showing the usefulness of the C-12/C-13 ratio as a chemistry indicator.

  20. Working Group 7.1 on environmental transport, US-USSR Joint Coordinating Committee on Civilian Nuclear Reactor Safety. Progress report update

    SciTech Connect

    Anspaugh, L.R.; Hendrickson, S.M.

    1991-09-07

    This report contains brief summaries of the status of projects of the Environmental Transport Group of the US-USSR Joint Coordinating Committee of Civilian Nuclear Reactor Safety. Projects reported on include: Management and Administration; Atmospheric Transport; Resuspension; External Dose; Terrestrial Food Chains; Aquatic Food Chains; Hydrological Transport; and Intercalibration

  1. Identification of Gifted and Creative Children and Youth Among Black Disadvantaged Groups. A Symposium/Workshop Presented by Members of the Bi-Racial Identification Committee.

    ERIC Educational Resources Information Center

    Torrance, E. Paul; And Others

    A symposium/workshop presented by members of the Bi-Racial Identification Committee dealt with the identification of the gifted and creative among black disadvantaged groups. The presentations were: (1) "Creative Positives of Black Disadvantaged Children" by E. Paul Torrance; (2) "Creativity As It Is Related to Expanding Educational Opportunities"…

  2. Breakup channels for C12 triple-α continuum states

    NASA Astrophysics Data System (ADS)

    Diget, C. Aa.; Barker, F. C.; Borge, M. J. G.; Boutami, R.; Dendooven, P.; Eronen, T.; Fox, S. P.; Fulton, B. R.; Fynbo, H. O. U.; Huikari, J.; Hyldegaard, S.; Jeppesen, H. B.; Jokinen, A.; Jonson, B.; Kankainen, A.; Moore, I.; Nieminen, A.; Nyman, G.; Penttilä, H.; Pucknell, V. F. E.; Riisager, K.; Rinta-Antila, S.; Tengblad, O.; Wang, Y.; Wilhelmsen, K.; Äystö, J.

    2009-09-01

    The triple-α-particle breakup of states in the triple-α continuum of C12 has been investigated by way of coincident detection of all three α particles of the breakup. The states have been fed in the β decay of N12 and B12, and the α particles measured using a setup that covers all of the triple-α phase space. Contributions from the breakup through the Be8(0+) ground state as well as other channels—interpreted as breakup through excited energies in Be8—have been identified. Spins and parities of C12 triple-α continuum states are deduced from the measured phase-space distributions for breakup through Be8 above the ground state by comparison to a fully symmetrized sequential R-matrix description of the breakup. At around 10 MeV in C12, the breakup is found to be dominated by 0+ strength breaking up through the ghost of the Be8(0+) ground state with L=0 angular momentum between the first emitted α particle and the intermediate Be8 nucleus. For C12 energies above the 12.7 MeV 1+ state, however, L=2 breakup of a C12 2+ state through the Be8(2+) excited state dominates. Furthermore, the possibility of a 2+ excited state in the 9-12 MeV region of C12 is investigated.

  3. Monitor unit calculations for external photon and electron beams: Report of the AAPM Therapy Physics Committee Task Group No. 71

    SciTech Connect

    Gibbons, John P.; Antolak, John A.; Followill, David S.; Huq, M. Saiful; Klein, Eric E.; Lam, Kwok L.; Palta, Jatinder R.; Roback, Donald M.; Reid, Mark; Khan, Faiz M.

    2014-03-15

    A protocol is presented for the calculation of monitor units (MU) for photon and electron beams, delivered with and without beam modifiers, for constant source-surface distance (SSD) and source-axis distance (SAD) setups. This protocol was written by Task Group 71 of the Therapy Physics Committee of the American Association of Physicists in Medicine (AAPM) and has been formally approved by the AAPM for clinical use. The protocol defines the nomenclature for the dosimetric quantities used in these calculations, along with instructions for their determination and measurement. Calculations are made using the dose per MU under normalization conditions, D{sub 0}{sup ′}, that is determined for each user's photon and electron beams. For electron beams, the depth of normalization is taken to be the depth of maximum dose along the central axis for the same field incident on a water phantom at the same SSD, where D{sub 0}{sup ′} = 1 cGy/MU. For photon beams, this task group recommends that a normalization depth of 10 cm be selected, where an energy-dependent D{sub 0}{sup ′} ≤ 1 cGy/MU is required. This recommendation differs from the more common approach of a normalization depth of d{sub m}, with D{sub 0}{sup ′} = 1 cGy/MU, although both systems are acceptable within the current protocol. For photon beams, the formalism includes the use of blocked fields, physical or dynamic wedges, and (static) multileaf collimation. No formalism is provided for intensity modulated radiation therapy calculations, although some general considerations and a review of current calculation techniques are included. For electron beams, the formalism provides for calculations at the standard and extended SSDs using either an effective SSD or an air-gap correction factor. Example tables and problems are included to illustrate the basic concepts within the presented formalism.

  4. Monitor unit calculations for external photon and electron beams: Report of the AAPM Therapy Physics Committee Task Group No. 71.

    PubMed

    Gibbons, John P; Antolak, John A; Followill, David S; Huq, M Saiful; Klein, Eric E; Lam, Kwok L; Palta, Jatinder R; Roback, Donald M; Reid, Mark; Khan, Faiz M

    2014-03-01

    A protocol is presented for the calculation of monitor units (MU) for photon and electron beams, delivered with and without beam modifiers, for constant source-surface distance (SSD) and source-axis distance (SAD) setups. This protocol was written by Task Group 71 of the Therapy Physics Committee of the American Association of Physicists in Medicine (AAPM) and has been formally approved by the AAPM for clinical use. The protocol defines the nomenclature for the dosimetric quantities used in these calculations, along with instructions for their determination and measurement. Calculations are made using the dose per MU under normalization conditions, D'0, that is determined for each user's photon and electron beams. For electron beams, the depth of normalization is taken to be the depth of maximum dose along the central axis for the same field incident on a water phantom at the same SSD, where D'0 = 1 cGy/MU. For photon beams, this task group recommends that a normalization depth of 10 cm be selected, where an energy-dependent D'0 ≤ 1 cGy/MU is required. This recommendation differs from the more common approach of a normalization depth of dm, with D'0 = 1 cGy/MU, although both systems are acceptable within the current protocol. For photon beams, the formalism includes the use of blocked fields, physical or dynamic wedges, and (static) multileaf collimation. No formalism is provided for intensity modulated radiation therapy calculations, although some general considerations and a review of current calculation techniques are included. For electron beams, the formalism provides for calculations at the standard and extended SSDs using either an effective SSD or an air-gap correction factor. Example tables and problems are included to illustrate the basic concepts within the presented formalism.

  5. Discussions and decisions of the 2012–2014 International Committee on Taxonomy of Viruses (ICTV) Filoviridae Study Group, January 2012–June 2013.

    PubMed

    Bukreyev, Alexander A; Chandran, Kartik; Dolnik, Olga; Dye, John M; Ebihara, Hideki; Leroy, Eric M; Mühlberger, Elke; Netesov, Sergey V; Patterson, Jean L; Paweska, Janusz T; Saphire, Erica Ollmann; Smither, Sophie J; Takada, Ayato; Towner, Jonathan S; Volchkov, Viktor E; Warren, Travis K; Kuhn, Jens H

    2014-04-01

    The International Committee on Taxonomy of Viruses (ICTV) Filoviridae Study Group prepares proposals on the classification and nomenclature of filoviruses to reflect current knowledge or to correct disagreements with the International Code of Virus Classification and Nomenclature (ICVCN). In recent years, filovirus taxonomy has been corrected and updated, but parts of it remain controversial, and several topics remain to be debated. This article summarizes the decisions and discussion of the currently acting ICTV Filoviridae Study Group since its inauguration in January 2012.

  6. Binary reaction decays from Mg24+C12

    NASA Astrophysics Data System (ADS)

    Beck, C.; Papka, P.; Zafra, A. Sànchez I.; Thummerer, S.; Azaiez, F.; Bednarczyk, P.; Courtin, S.; Curien, D.; Dorvaux, O.; Lebhertz, D.; Nourreddine, A.; Rousseau, M.; Von Oertzen, W.; Gebauer, B.; Wheldon, C.; Kokalova, Tz.; De Angelis, G.; Gadea, A.; Lenzi, S.; Szilner, S.; Napoli, D. R.; Catford, W. N.; Jenkins, D. G.; Royer, G.

    2009-09-01

    Charged-particle and γ decays in Mg24* are investigated for excitation energies where quasimolecular resonances appear in C12+C12 collisions. Various theoretical predictions for the occurrence of superdeformed and hyperdeformed bands associated with resonance structures with low spin are discussed within the measured Mg24* excitation energy region. The inverse kinematics reaction Mg24+C12 is studied at Elab(Mg24)=130 MeV, an energy that enables the population of Mg24 states decaying into C12+C12 resonant breakup states. Exclusive data were collected with the Binary Reaction Spectrometer in coincidence with Euroball IV installed at the Vivitron tandem facility at Strasbourg. Specific structures with large deformation were selectively populated in binary reactions, and their associated γ decays studied. Coincident events associated with inelastic and α-transfer channels have been selected by choosing the excitation energy or the entry point via the two-body Q values. The analysis of the binary reaction channels is presented with a particular emphasis on Mg24-γ, Ne20-γ, and O16-γ coincidences. New information (spin and branching ratios) is deduced on high-energy states in Mg24 and O16, respectively.

  7. Cyberinfrastructure Initiatives of the Committee on Earth Observation Satellites (CEOS) Working Group on Information Systems and Services (WGISS)

    NASA Astrophysics Data System (ADS)

    McDonald, K. R.; Faundeen, J. L.; Petiteville, I.

    2005-12-01

    The Committee on Earth Observation Satellites (CEOS) was established in 1984 in response to a recommendation from the Economic Summit of Industrialized Nations Working Group on Growth, Technology, and Employment's Panel of Experts on Satellite Remote Sensing. CEOS participants are Members, who are national or international governmental organizations who operate civil spaceborne Earth observation satellites, and Associates who are governmental organizations with civil space programs in development or international scientific or governmental bodies who have an interest in and support CEOS objectives. The primary objective of CEOS is to optimize benefits of satellite Earth observations through cooperation of its participants in mission planning and in development of compatible data products, formats, services, applications and policies. To pursue its objectives, CEOS establishes working groups and associated subgroups that focus on relevant areas of interest. While the structure of CEOS has evolved over its lifetime, today there are three permanent working groups. One is the Working Group on Calibration and Validation that addresses sensor-specific calibration and validation and geophysical parameter validation. A second is the Working Group on Education, Training, and Capacity Building that facilitates activities that enhance international education and training in Earth observation techniques, data analysis, interpretation and applications, with a particular focus on developing countries. The third permanent working group is the Working Group on Information Systems and Services (WGISS). The purpose of WGISS is to promote collaboration in the development of the systems and services based on international standards that manage and supply the Earth observation data and information from participating agencies' missions. WGISS places great emphasis on the use of demonstration projects involving user groups to solve the critical interoperability issues associated with the

  8. Health care reform and Diagnosis Related Groups in Germany: The mediating role of Hospital Liaison Committees for Jehovah's Witnesses.

    PubMed

    Rajtar, Małgorzata

    2016-10-01

    Resulting from health care reform in Germany that was implemented in 2003-2004, a new medical classification system called the "Diagnosis Related Groups" (DRGs) was introduced in hospitals. According to the media, social scientists, and a few physicians interviewed in this study the policy negatively transformed the German health care system by allowing the privatization of the hospital sector consistent with the neoliberal health care model. Allegedly, this privileged economic values over the quality of health care and introduced competition between hospitals. Nevertheless, members of the Hospital Liaison Committees (HLCs) of Jehovah's Witnesses argued that the DRGs system could be used to the advantage of Jehovah's Witness (JW) patients. HLCs often assist in the patient's search by providing names of physicians that would be willing to refrain from blood transfusions. This article draws from nine months of ethnographic research with Jehovah's Witnesses, including members of the HLCs, carried out primarily in Berlin between 2010 and 2012. By focusing on JWs, whose refusal of blood transfusions is often exemplified as particularly difficult for the biomedical profession, it addresses the "unintended" consequences of the introduction of DRGs into the German health care system that remain unexplored by health and social science scholarship. It argues that although JWs have long been associated with the judicialization of religious freedom globally, they do not equally engage in the judicialization of health in countries such as Germany. The reason for this is embedded not only in health care policy that favors mediation over medical malpractice litigation. It also results from the synergy of health care reforms that prioritize standardizing and economizing measures such as DRGs as well as practices implemented by Patient Blood Management programs that JW institutions, such as HLCs, have tapped into. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Nutritional strategy for adolescents undergoing bariatric surgery: report of a working group of the Nutrition Committee of NASPGHAN/NACHRI.

    PubMed

    Fullmer, Michell A; Abrams, Stephanie H; Hrovat, Kathleen; Mooney, Lori; Scheimann, Ann O; Hillman, Jennifer B; Suskind, David L

    2012-01-01

    Surgical options for the treatment of adolescent obesity have been gaining popularity. Adolescent patients present a particular challenge to clinicians, secondary to age-related issues, revolving around both mental and physical growth. These age-related issues require a unique approach to nutritional intervention for adolescents undergoing bariatric surgery as opposed to standardized approaches for adults. Despite the increasing numbers of adolescents undergoing obesity surgery, evidence-based nutritional guidelines have yet to be published. The goal of this document is to provide the clinician with recommendations on how to assess, educate, nourish, and monitor the adolescent who has undergone obesity surgery. A multidisciplinary panel composed of 3 pediatric gastroenterologists, 1 psychologist, and 3 registered dietitians from the Nutrition Committee for the North American Society of Pediatric Gastroenterology, Hepatology, and Nutrition and National Association of Children's Hospitals and Related Institutions, with experience in nutrition and adolescent weight loss surgery, reviewed the medical literature for evidence-based practice for nutritional strategies for patients undergoing bariatric surgery. In addition to this group, an adolescent medicine physician was consulted for matters related to reproductive health. The present article presents a consensus of recommendations based on a review of the literature. In areas for which there was a lack of evidence to support the recommendations, best-practice guidelines were used. The present article provides the clinician with an overview of the nutritional concerns for adolescent patients undergoing obesity surgery. These guidelines address the preoperative educational pathway, the postoperative diet progression, recognition of disordered eating, guidelines for female reproductive issues, and assistance for the adolescent in a school/college environment.

  10. Cluster Emission in 13C + 12C and 12C + 12C Reactions at ~ 6 Mev/nucleon

    NASA Astrophysics Data System (ADS)

    Rana, T. K.; Bhattacharya, C.; Kundu, S.; Banerjee, K.; Bhattacharya, S.; Mukherjee, G.; Ghosh, T. K.; Meena, J. K.; Dhara, P.; Biswas, M.; Pai, H.; Mahata, K.; Kumar, Suresh; Ramachandran, K.; Rout, P. C.; Pandit, S. K.; Nanal, V.; Pillay, R. G.

    Cluster state formation viz. the population of unbound 8Be and the Hoyle state of 12C produced in the reactions 12C(77 MeV) + 12C and 13C(75 MeV) + 12C have been studied using resonance particle spectroscopy. It was observed that there is a large difference in the cluster state formation in these two reactions and the yield of neutron rich isotopes of different fragments is more in the 13C + 12C compared to 12C + 12C reactions at 6 MeV/u.

  11. Report on the activities of the ASME-NQA Committee Working Group on Quality Assurance Requirements for Research and Development, April 1990 to August 1991

    SciTech Connect

    Dronkers, J.J.

    1991-09-01

    This report transmits to the public eye the activities of the American Society of Mechanical Engineers-Nuclear Quality Assurance (ASME-NQA) Committee Working Group on Quality Assurance Requirements for Research and Development. The appendix lists the members of this group as of August 1991. The report covers a period of 17 months. The working group met eight times in this period, and much intellectual ground was traversed. There was seldom agreement on the nature of the task, but there was no doubt as to its urgency. The task was how to adapt the nuclear quality assurance standard, the NQA-1, to research and development work. 1 fig., 7 tabs.

  12. Committee of the Undergraduate Program in Mathematics Geometry Conference, Part III: Geometric Transformation Groups and Other Topics.

    ERIC Educational Resources Information Center

    Durst, Lincoln K., Ed.

    This is Part III of the first volume of the proceedings of the Committee on the Undergraduate Program in Mathematics (CUPM) Geometry Conference, held at Santa Barbara in June, 1967. The purpose of the conference was to consider the status of geometry in colleges at the undergraduate level. The conference, attended by undergraduate mathematics…

  13. 75 FR 51827 - Notice of a Meeting of a Working Group of the NIH Advisory Committee to the Director

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-23

    ... inform the public about a meeting of the NIH Blue Ribbon Panel to Advise on the Risk Assessment of the... public meetings between the Blue Ribbon Panel and the National Research Council Committee on Technical... concerning this meeting, contact Ms. Kelly Fennington, Senior Health Policy Analyst, Office of Biotechnology...

  14. 40 CFR 721.3025 - Fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters (generic).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Fatty acids C12-18, C18 unsaturated... Significant New Uses for Specific Chemical Substances § 721.3025 Fatty acids C12-18, C18 unsaturated, C12-18... chemical substance identified generically as fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters...

  15. 40 CFR 721.3025 - Fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters (generic).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Fatty acids C12-18, C18 unsaturated... Significant New Uses for Specific Chemical Substances § 721.3025 Fatty acids C12-18, C18 unsaturated, C12-18... chemical substance identified generically as fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters...

  16. High-spin molecular resonances in 12C + 12C

    NASA Astrophysics Data System (ADS)

    Uegaki, E.; Abe, Y.

    2016-05-01

    Resonances observed in the 12C + 12C collisions are studied with a molecular model. At high spins J = 10-18, a stable dinuclear configuration is found to be an equator-equator touching one. Firstly, normal modes have been solved around the equilibrium, with spin J and K-quantum number being specified for rotation of the whole system. Secondly, with respect to large centrifugal energy, Coriolis coupling has been diagonalized among low-lying 11 states of normal-mode excitations, which brings K-mixing. The analyses of decay widths and excitation functions have been done. The molecular ground state exhibits alignments of the orbital angular momentum and the 12C spins, while some of the molecular excited states exhibit disalignments with small widths. Those results are surprisingly in good agreement with the experiments, which will light up a new physical picture of the highspin 12C + 12C resonances.

  17. Monitor unit calculations for external photon and electron beams: Report of the AAPM Therapy Physics Committee Task Group No. 71

    PubMed Central

    Gibbons, John P.; Antolak, John A.; Followill, David S.; Huq, M. Saiful; Klein, Eric E.; Lam, Kwok L.; Palta, Jatinder R.; Roback, Donald M.; Reid, Mark; Khan, Faiz M.

    2014-01-01

    A protocol is presented for the calculation of monitor units (MU) for photon and electron beams, delivered with and without beam modifiers, for constant source-surface distance (SSD) and source-axis distance (SAD) setups. This protocol was written by Task Group 71 of the Therapy Physics Committee of the American Association of Physicists in Medicine (AAPM) and has been formally approved by the AAPM for clinical use. The protocol defines the nomenclature for the dosimetric quantities used in these calculations, along with instructions for their determination and measurement. Calculations are made using the dose per MU under normalization conditions, \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{upgreek} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} }{}$D_0^\\prime $\\end{document}D0′, that is determined for each user's photon and electron beams. For electron beams, the depth of normalization is taken to be the depth of maximum dose along the central axis for the same field incident on a water phantom at the same SSD, where \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{upgreek} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} }{}$D_0^\\prime $\\end{document}D0′ = 1 cGy/MU. For photon beams, this task group recommends that a normalization depth of 10 cm be selected, where an energy-dependent \\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{upgreek} \\usepackage{mathrsfs} \\setlength{\\oddsidemargin}{-69pt} \\begin{document} }{}$D_0^\\prime $\\end{document}D0′ ≤ 1 cGy/MU is required. This recommendation differs from the more common approach of a normalization depth of dm, with

  18. CCCT - Patient Advocate Steering Committee

    Cancer.gov

    The Patient Advocate Steering Committee (PASC) works to ensure advocates involved with the Scientific Steering Committees (SSCs) are completely integrated in the development, implementation, and monitoring of clinical trials within those groups.

  19. [Bioethics committees].

    PubMed

    Lanzilota, Alicia

    2007-01-01

    The present paper exposes a brief historical route showing the context of the Ethics Committee developing. The moral conflicts in clinical care that doctors had to face with the introduction of the new technologies, the creation of the first clinical committees in USA and the abuses in the biomedical investigations, determined the establishment of ethical guidelines and settled institutional review committees for its control. First Bioethics Committees in Argentina were established by adopting the US Ethic Committees model. The proliferation of hospital ethics committees was related to local regulations requirements. Current regulations. Bioethics Committees in Buenos Aires City, through the data of a survey.

  20. Congenital and acquired neutropenia consensus guidelines on diagnosis from the Neutropenia Committee of the Marrow Failure Syndrome Group of the AIEOP (Associazione Italiana Emato-Oncologia Pediatrica).

    PubMed

    Fioredda, Francesca; Calvillo, Michaela; Bonanomi, Sonia; Coliva, Tiziana; Tucci, Fabio; Farruggia, Piero; Pillon, Marta; Martire, Baldassarre; Ghilardi, Roberta; Ramenghi, Ugo; Renga, Daniela; Menna, Giuseppe; Barone, Angelica; Lanciotti, Marina; Dufour, Carlo

    2011-07-15

    Congenital and acquired neutropenia are rare disorders whose frequency in pediatric age may be underestimated due to remarkable differences in definition or misdiagnosed because of the lack of common practice guidelines. Neutropenia Committee of the Marrow Failure Syndrome Group (MFSG) of the AIEOP (Associazione Italiana Emato-Oncologia Pediatrica) elaborated this document following design and methodology formerly approved by the AIEOP board. The panel of experts reviewed the literature on the topic and participated in a conference producing a document which includes a classification of neutropenia and a comprehensive guideline on diagnosis of neutropenia. Copyright © 2011 Wiley-Liss, Inc.

  1. 40 CFR 721.3025 - Fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters (generic).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., C12-18 alkyl esters (generic). 721.3025 Section 721.3025 Protection of Environment ENVIRONMENTAL... alkyl esters (generic). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters (PMNs...

  2. 40 CFR 721.3025 - Fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters (generic).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., C12-18 alkyl esters (generic). 721.3025 Section 721.3025 Protection of Environment ENVIRONMENTAL... alkyl esters (generic). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters (PMNs...

  3. 40 CFR 721.3025 - Fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters (generic).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., C12-18 alkyl esters (generic). 721.3025 Section 721.3025 Protection of Environment ENVIRONMENTAL... alkyl esters (generic). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically as fatty acids C12-18, C18 unsaturated, C12-18 alkyl esters...

  4. The C-12/C-13 abundance ratio in Comet Halley

    SciTech Connect

    Wyckoff, S.; Lindholm, E.; Wehinger, P.A.; Peterson, B.A.; Zucconi, J.M.

    1989-04-01

    The individual (C-13)N rotational lines in Comet Halley are resolved using high-resolution spectra of the CN B2Sigma(+)-X2Sigma(+) (0,0) band. The observe C-12/C-13 abundance ratio excludes a site of origin for the comet near Uranus and Neptune and suggests a condensation environment quite distinct from other solar system bodies. Two theories are presented for the origin of Comet Halley. One theory suggest that the comet originated 4.5 Gyr ago in an inner Oort cloud at a heliocentric distance greater than 100 AU where chemical fractionation led to the C-13 enrichment in the CN parent molecule prior to condensation of the comet nucleus. According to the other, more plausible theory, the comet nucleus condensed relatively recently from the interstellar medium which has become enriches in C-13 and was subsequently gravitationally captured by the solar system. 107 refs.

  5. Intermediate resonance of inelastic 12C + 12C scattering

    NASA Astrophysics Data System (ADS)

    Osamu, Tanimura

    1980-01-01

    The intermediate resonances observed in the inelastic 12C + 12C cross sections to the single and mutual 2 1+(4.43 MeV) excitations and the single 3 1- (9.64 MeV) excitation are studied by the coupled-channel method with the use of the coupling interaction derived by the folding procedure between 12C and 12C. It is shown that the model is successful in reproducing the gross structures of the inelastic cross sections and especially the correlated resonance energies of the inelastic channels. The inelastic resonances are shown to be due to the molecular resonances in an adiabatic potential between two 12C, which reproduces correctly the coupled channel resonances.

  6. Conference Committees: Conference Committees

    NASA Astrophysics Data System (ADS)

    2009-09-01

    International Programm Committee (IPC) Harald Ade NCSU Sadao Aoki University Tsukuba David Attwood Lawrence Berkeley National Laboratory/CXRO Christian David Paul Scherrer Institut Peter Fischer Lawrence Berkeley National Laboratory Adam Hitchcock McMaster University Chris Jacobsen SUNY, Stony Brook Denis Joyeux Lab Charles Fabry de l'Institut d'Optique Yasushi Kagoshima University of Hyogo Hiroshi Kihara Kansai Medical University Janos Kirz SUNY Stony Brook Maya Kiskinova ELETTRA Ian McNulty Argonne National Lab/APS Alan Michette Kings College London Graeme Morrison Kings College London Keith Nugent University of Melbourne Zhu Peiping BSRF Institute of High Energy Physics Francois Polack Soleil Christoph Quitmann Paul Scherrer Institut Günther Schmahl University Göttingen Gerd Schneider Bessy Hyun-Joon Shin Pohang Accelerator Lab Jean Susini ESRF Mau-Tsu Tang NSRRC Tony Warwick Lawrence Berkeley Lab/ALS Local Organizing Committee Christoph Quitmann Chair, Scientific Program Charlotte Heer Secretary Christian David Scientific Program Frithjof Nolting Scientific Program Franz Pfeiffer Scientific Program Marco Stampanoni Scientific Program Robert Rudolph Sponsoring, Financials Alfred Waser Industry Exhibition Robert Keller Public Relation Markus Knecht Computing and WWW Annick Cavedon Proceedings and Excursions and Accompanying Persons Program Margrit Eichler Excursions and Accompanying Persons Program Kathy Eikenberry Excursions and Accompanying Persons Program Marlies Locher Excursions and Accompanying Persons Program

  7. Report on Focus Groups Conducted for the Prichard Committee for Academic Excellence and the Partnership for Kentucky School Reform.

    ERIC Educational Resources Information Center

    Roberts, Rona; Kay, Steve

    Findings of a study that explored focus group participants' views on public schools and Kentucky's recent education reform efforts are presented in this document. In May 1992, 7 focus groups, with a total of 70 participants, were conducted at 6 locations throughout Kentucky. Three groups consisted of citizens who were not public school employees,…

  8. 77 FR 14584 - Eleventh Meeting: RTCA Special Committee 217, Joint With EUROCAE Working Group-44, Terrain and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-12

    ... Group--44, Terrain and Airport Mapping Databases AGENCY: Federal Aviation Administration (FAA), U.S... Working Group--44, Terrain and Airport Mapping Databases. SUMMARY: The FAA is issuing this notice to...--44, Terrain and Airport Mapping Databases. DATES: The meeting will be held April 2-5, 2012, from 9...

  9. Bioenergetic Profile Experiment using C2C12 Myoblast Cells

    PubMed Central

    Nicholls, David G.; Darley-Usmar, Victor M.; Wu, Min; Jensen, Per Bo; Rogers, George W.; Ferrick, David A.

    2010-01-01

    The ability to measure cellular metabolism and understand mitochondrial dysfunction, has enabled scientists worldwide to advance their research in understanding the role of mitochondrial function in obesity, diabetes, aging, cancer, cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, fatty acids, and glutamine, and subsequent energy conversion through a series of enzymatically controlled oxidation and reduction reactions. These intracellular biochemical reactions result in the production of ATP, the release of heat and chemical byproducts, such as lactate and CO2 into the extracellular environment. Valuable insight into the physiological state of cells, and the alteration of the state of those cells, can be gained through measuring the rate of oxygen consumed by the cells, an indicator of mitochondrial respiration - the Oxygen Consumption Rate - or OCR. Cells also generate ATP through glycolysis, i.e.: the conversion of glucose to lactate, independent of oxygen. In cultured wells, lactate is the primary source of protons. Measuring the lactic acid produced indirectly via protons released into the extracellular medium surrounding the cells, which causes acidification of the medium provides the Extra-Cellular Acidification Rate - or ECAR. In this experiment, C2C12 myoblast cells are seeded at a given density in Seahorse cell culture plates. The basal oxygen consumption (OCR) and extracellular acidification (ECAR) rates are measured to establish baseline rates. The cells are then metabolically perturbed by three additions of different compounds (in succession) that shift the bioenergetic profile of the cell. This assay is derived from a classic experiment to assess mitochondria and serves as a framework with which to build more complex experiments aimed at understanding both physiologic and pathophysiologic function of mitochondria and to predict the ability of cells to respond to stress and

  10. Bioenergetic profile experiment using C2C12 myoblast cells.

    PubMed

    Nicholls, David G; Darley-Usmar, Victor M; Wu, Min; Jensen, Per Bo; Rogers, George W; Ferrick, David A

    2010-12-06

    The ability to measure cellular metabolism and understand mitochondrial dysfunction, has enabled scientists worldwide to advance their research in understanding the role of mitochondrial function in obesity, diabetes, aging, cancer, cardiovascular function and safety toxicity. Cellular metabolism is the process of substrate uptake, such as oxygen, glucose, fatty acids, and glutamine, and subsequent energy conversion through a series of enzymatically controlled oxidation and reduction reactions. These intracellular biochemical reactions result in the production of ATP, the release of heat and chemical byproducts, such as lactate and CO(2) into the extracellular environment. Valuable insight into the physiological state of cells, and the alteration of the state of those cells, can be gained through measuring the rate of oxygen consumed by the cells, an indicator of mitochondrial respiration--the Oxygen Consumption Rate--or OCR. Cells also generate ATP through glycolysis, i.e.: the conversion of glucose to lactate, independent of oxygen. In cultured wells, lactate is the primary source of protons. Measuring the lactic acid produced indirectly via protons released into the extracellular medium surrounding the cells, which causes acidification of the medium provides the Extra-Cellular Acidification Rate--or ECAR. In this experiment, C2C12 myoblast cells are seeded at a given density in Seahorse cell culture plates. The basal oxygen consumption (OCR) and extracellular acidification (ECAR) rates are measured to establish baseline rates. The cells are then metabolically perturbed by three additions of different compounds (in succession) that shift the bioenergetic profile of the cell. This assay is derived from a classic experiment to assess mitochondria and serves as a framework with which to build more complex experiments aimed at understanding both physiologic and pathophysiologic function of mitochondria and to predict the ability of cells to respond to stress and

  11. Report of the International Telegraph and Telephone Consultative Committee Study Group. XVIII. Group of Experts on ISDN Matters Meeting in Kyoto, Japan.

    DTIC Science & Technology

    1983-02-01

    033 2. Definitions A.033 3. Relationships A.035 Annex B (at page A33) A.035 Annex 9 Proposed Revisions to Draft Recommendation I.XXX A.037 Examples...of Implementation of NT1 and NT2 Functions A.038 Annex 10 Proposed Revisions to Draft Recommendation I.XXY A.042 Annex 8 Reply to SGVII on Number of R...ordination meeting has revised the agenda for the group of experts meeting as shown in Annex 1. In parallel with the meeting of the Group of experts on ISDN

  12. Differential sensitivity of C2-C12 striated muscle cells to lovastatin and pravastatin.

    PubMed

    Gadbut, A P; Caruso, A P; Galper, J B

    1995-10-01

    One of the major side-effects of the use of HMG CoA reductase inhibitors for the treatment of hypercholesterolemia is the development of myositis and, in some patients undergoing concomitant immunosuppressive treatment, the development of rhabdomyolysis. Experiments outlined in these studies demonstrate that inhibitors of HMG-CoA reductase activity which differ primary in the substitution of a methyl group for a hydroxyl group have differential effects on both cholesterol levels and cell viability in a striated muscle cell model, the mouse C2-C12 myoblast. Thus, concentrations as high as 200 microM of pravastatin had little effect on total cholesterol level while 25 microM of lovastatin decreased cellular cholesterol by over 90%. Simvastatin and lovastatin decreased viability of C2-C12 myoblasts by nearly 50% at concentrations as low as 1 and 5 microM, respectively, and decreased viability by almost 90% at 10 and 15 microM respectively. However, 300 microM of pravastatin decreased cell viability by less than 50%. The order of potency for the effects on cell viability wassimvastatin>lovastatin>pravastatin. The possible relationship between effects on cell viability and the development of myositis is discussed.

  13. 40 CFR 721.644 - Amines, C12-14-tert-alkyl, sulfonates.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Amines, C12-14-tert-alkyl, sulfonates... Substances § 721.644 Amines, C12-14-tert-alkyl, sulfonates. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as amines, C12-14-tert-alkyl, sulfonates...

  14. 40 CFR 721.644 - Amines, C12-14-tert-alkyl, sulfonates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Amines, C12-14-tert-alkyl, sulfonates... Substances § 721.644 Amines, C12-14-tert-alkyl, sulfonates. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as amines, C12-14-tert-alkyl, sulfonates...

  15. 40 CFR 721.10007 - Alcohols, C12-14-secondary, ethoxylated propoxylated.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Alcohols, C12-14-secondary... New Uses for Specific Chemical Substances § 721.10007 Alcohols, C12-14-secondary, ethoxylated... identified as alcohols, C12-14- secondary, ethoxylated propoxylated (PMN P-00-11; CAS No. 103331-86-8)...

  16. 40 CFR 721.10007 - Alcohols, C12-14-secondary, ethoxylated propoxylated.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Alcohols, C12-14-secondary... New Uses for Specific Chemical Substances § 721.10007 Alcohols, C12-14-secondary, ethoxylated... identified as alcohols, C12-14- secondary, ethoxylated propoxylated (PMN P-00-11; CAS No. 103331-86-8)...

  17. 40 CFR 721.10007 - Alcohols, C12-14-secondary, ethoxylated propoxylated.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Alcohols, C12-14-secondary... New Uses for Specific Chemical Substances § 721.10007 Alcohols, C12-14-secondary, ethoxylated... identified as alcohols, C12-14- secondary, ethoxylated propoxylated (PMN P-00-11; CAS No. 103331-86-8)...

  18. Orbital Sarcoma with Metastases at Diagnosis: A Report from the Soft Tissue Sarcoma Committee of the Children's Oncology Group

    PubMed Central

    Huh, Winston W.; Anderson, James R.; Rodeberg, David; Teot, Lisa; Yock, Torunn; Raney, R. Beverly

    2011-01-01

    We reviewed clinicopathologic features and treatment outcomes in 7 patients diagnosed with Stage 4/Group IV orbital sarcoma and treated on IRSG protocols I-III. Three patients had embryonal rhabdomyosarcoma (RMS), and 2 patients each had alveolar RMS or unclassified sarcoma. Median age at diagnosis was 1.8 years (range 0.2 – 6.9 years). All patients had bone marrow involvement, including 6 with normal CBC at diagnosis. Cerebrospinal fluid (CSF) was normal in 6 patients. Three patients survived > 5 years, including one with local recurrence. Conclusion Further study is needed to determine necessity of bone marrow and CSF examination in orbital sarcoma patients. PMID:20162686

  19. Intraoperative radiation therapy using mobile electron linear accelerators: report of AAPM Radiation Therapy Committee Task Group No. 72.

    PubMed

    Beddar, A Sam; Biggs, Peter J; Chang, Sha; Ezzell, Gary A; Faddegon, Bruce A; Hensley, Frank W; Mills, Michael D

    2006-05-01

    Intraoperative radiation therapy (IORT) has been customarily performed either in a shielded operating suite located in the operating room (OR) or in a shielded treatment room located within the Department of Radiation Oncology. In both cases, this cancer treatment modality uses stationary linear accelerators. With the development of new technology, mobile linear accelerators have recently become available for IORT. Mobility offers flexibility in treatment location and is leading to a renewed interest in IORT. These mobile accelerator units, which can be transported any day of use to almost any location within a hospital setting, are assembled in a nondedicated environment and used to deliver IORT. Numerous aspects of the design of these new units differ from that of conventional linear accelerators. The scope of this Task Group (TG-72) will focus on items that particularly apply to mobile IORT electron systems. More specifically, the charges to this Task Group are to (i) identify the key differences between stationary and mobile electron linear accelerators used for IORT, (ii) describe and recommend the implementation of an IORT program within the OR environment, (iii) present and discuss radiation protection issues and consequences of working within a nondedicated radiotherapy environment, (iv) describe and recommend the acceptance and machine commissioning of items that are specific to mobile electron linear accelerators, and (v) design and recommend an efficient quality assurance program for mobile systems.

  20. t-10, c-12 CLA Dietary Supplementation Inhibits Atherosclerotic Lesion Development Despite Adverse Cardiovascular and Hepatic Metabolic Marker Profiles

    PubMed Central

    Mitchell, Patricia L.; Karakach, Tobias K.; Currie, Deborah L.; McLeod, Roger S.

    2012-01-01

    Animal and human studies have indicated that fatty acids such as the conjugated linoleic acids (CLA) found in milk could potentially alter the risk of developing metabolic disorders including diabetes and cardiovascular disease (CVD). Using susceptible rodent models (apoE−/− and LDLr−/− mice) we investigated the interrelationship between mouse strain, dietary conjugated linoleic acids and metabolic markers of CVD. Despite an adverse metabolic risk profile, atherosclerosis (measured directly by lesion area), was significantly reduced with t-10, c-12 CLA and mixed isomer CLA (Mix) supplementation in both apoE−/− (p<0.05, n = 11) and LDLr−/− mice (p<0.01, n = 10). Principal component analysis was utilized to delineate the influence of multiple plasma and tissue metabolites on the development of atherosclerosis. Group clustering by dietary supplementation was evident, with the t-10, c-12 CLA supplemented animals having distinct patterns, suggestive of hepatic insulin resistance, regardless of mouse strain. The effect of CLA supplementation on hepatic lipid and fatty acid composition was explored in the LDLr−/− strain. Dietary supplementation with t-10, c-12 CLA significantly increased liver weight (p<0.05, n = 10), triglyceride (p<0.01, n = 10) and cholesterol ester content (p<0.01, n = 10). Furthermore, t-10, c-12 CLA also increased the ratio of 18∶1 to 18∶0 fatty acid in the liver suggesting an increase in the activity of stearoyl-CoA desaturase. Changes in plasma adiponectin and liver weight with t-10, c-12 CLA supplementation were evident within 3 weeks of initiation of the diet. These observations provide evidence that the individual CLA isomers have divergent mechanisms of action and that t-10, c-12 CLA rapidly changes plasma and liver markers of metabolic syndrome, despite evidence of reduction in atherosclerosis. PMID:23285120

  1. t-10, c-12 CLA dietary supplementation inhibits atherosclerotic lesion development despite adverse cardiovascular and hepatic metabolic marker profiles.

    PubMed

    Mitchell, Patricia L; Karakach, Tobias K; Currie, Deborah L; McLeod, Roger S

    2012-01-01

    Animal and human studies have indicated that fatty acids such as the conjugated linoleic acids (CLA) found in milk could potentially alter the risk of developing metabolic disorders including diabetes and cardiovascular disease (CVD). Using susceptible rodent models (apoE(-/-) and LDLr(-/-) mice) we investigated the interrelationship between mouse strain, dietary conjugated linoleic acids and metabolic markers of CVD. Despite an adverse metabolic risk profile, atherosclerosis (measured directly by lesion area), was significantly reduced with t-10, c-12 CLA and mixed isomer CLA (Mix) supplementation in both apoE(-/-) (p<0.05, n = 11) and LDLr(-/-) mice (p<0.01, n = 10). Principal component analysis was utilized to delineate the influence of multiple plasma and tissue metabolites on the development of atherosclerosis. Group clustering by dietary supplementation was evident, with the t-10, c-12 CLA supplemented animals having distinct patterns, suggestive of hepatic insulin resistance, regardless of mouse strain. The effect of CLA supplementation on hepatic lipid and fatty acid composition was explored in the LDLr(-/-) strain. Dietary supplementation with t-10, c-12 CLA significantly increased liver weight (p<0.05, n = 10), triglyceride (p<0.01, n = 10) and cholesterol ester content (p<0.01, n = 10). Furthermore, t-10, c-12 CLA also increased the ratio of 18∶1 to 18∶0 fatty acid in the liver suggesting an increase in the activity of stearoyl-CoA desaturase. Changes in plasma adiponectin and liver weight with t-10, c-12 CLA supplementation were evident within 3 weeks of initiation of the diet. These observations provide evidence that the individual CLA isomers have divergent mechanisms of action and that t-10, c-12 CLA rapidly changes plasma and liver markers of metabolic syndrome, despite evidence of reduction in atherosclerosis.

  2. 7 CFR 610.24 - Responsibilities of State Technical Committees.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Program, Conservation Innovation Grants, Cooperative Conservation Partnership Initiative, Agricultural... Committee's recommendations. (c) State Technical Committees will review whether Local Working Groups are...

  3. 7 CFR 610.24 - Responsibilities of State Technical Committees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Program, Conservation Innovation Grants, Cooperative Conservation Partnership Initiative, Agricultural... Committee's recommendations. (c) State Technical Committees will review whether Local Working Groups are...

  4. Refining American Joint Committee on Cancer/Union for International Cancer Control TNM stage and prognostic groups for human papillomavirus-related oropharyngeal carcinomas.

    PubMed

    Huang, Shao Hui; Xu, Wei; Waldron, John; Siu, Lillian; Shen, Xiaowei; Tong, Li; Ringash, Jolie; Bayley, Andrew; Kim, John; Hope, Andrew; Cho, John; Giuliani, Meredith; Hansen, Aaron; Irish, Jonathan; Gilbert, Ralph; Gullane, Patrick; Perez-Ordonez, Bayardo; Weinreb, Ilan; Liu, Fei-Fei; O'Sullivan, Brian

    2015-03-10

    edition TNM, and prognostication is further improved by an RPA-based prognostic grouping within the American Joint Committee on Cancer/Union for International Cancer Control TNM framework for HPV-related OPC. © 2015 by American Society of Clinical Oncology.

  5. Intracranial stereotactic positioning systems: Report of the American Association of Physicists in Medicine Radiation Therapy Committee Task Group No. 68

    SciTech Connect

    Lightstone, A.W.; Benedict, Stanley H.; Bova, Frank J.; Solberg, Timothy D.; Stern, Robin L.

    2005-07-15

    Intracranial stereotactic positioning systems (ISPSs) are used to position patients prior to precise radiation treatment of localized lesions of the brain. Often, the lesion is located in close proximity to critical anatomic features whose functions should be maintained. Many types of ISPSs have been described in the literature and are commercially available. These are briefly reviewed. ISPS systems provide two critical functions. The first is to establish a coordinate system upon which a guided therapy can be applied. The second is to provide a method to reapply the coordinate system to the patient such that the coordinates assigned to the patient's anatomy are identical from application to application. Without limiting this study to any particular approach to ISPSs, this report introduces nomenclature and suggests performance tests to quantify both the stability of the ISPS to map diagnostic data to a coordinate system, as well as the ISPS's ability to be realigned to the patient's anatomy. For users who desire to develop a new ISPS system, it may be necessary for the clinical team to establish the accuracy and precision of each of these functions. For commercially available systems that have demonstrated an acceptable level of accuracy and precision, the clinical team may need to demonstrate local ability to apply the system in a manner consistent with that employed during the published testing. The level of accuracy and precision required of an individual ISPS system is dependent upon the clinical protocol (e.g., fractionation, margin, pathology, etc.). Each clinical team should provide routine quality assurance procedures that are sufficient to support the assumptions of accuracy and precision used during the planning process. The testing of ISPS systems can be grouped into two broad categories, type testing, which occurs prior to general commercialization, and site testing, performed when a commercial system is installed at a clinic. Guidelines to help select

  6. Unrelated adult stem cell donor medical suitability: recommendations from the World Marrow Donor Association Clinical Working Group Committee.

    PubMed

    Lown, R N; Philippe, J; Navarro, W; van Walraven, S M; Philips-Johnson, L; Fechter, M; Pawson, R; Bengtsson, M; Beksac, M; Field, S; Yang, H; Shaw, B E

    2014-07-01

    The World Marrow Donor Association (WMDA) fosters collaboration between international registries to facilitate the exchange of hematopoietic stem cell products for unrelated stem cell donor transplantation. As indications for hematopoietic SCT grow, the movement of products across the world will increase. Although competent authorities may regulate products within their country, there is a need to protect the best interests of donors and recipients by identifying universal donor medical suitability criteria. Within this report the WMDA provides a background to unrelated adult donor and recipient safety, recommends a common framework for assessing the health of unrelated adult donors at each stage of the donation pathway and presents a novel mechanism for sharing international consensus criteria for individual medical and lifestyle conditions. Wherever possible, these criteria are evidence-based. By establishing a donor medical suitability working group, the WMDA has developed a process through which donor centers and registries may request a consensus opinion on conditions not already listed, as well as challenge existing criteria. Guidance from the WMDA is intended to complement, not supersede, guidance from national competent authorities and international regulatory bodies.

  7. Astragalus Polysaccharide Inhibits Autophagy and Apoptosis from Peroxide-Induced Injury in C2C12 Myoblasts.

    PubMed

    Yin, Yi; Lu, Lu; Wang, Dongtao; Shi, Ying; Wang, Ming; Huang, Yanfeng; Chen, Dexiu; Deng, Cong; Chen, Jiebin; Lv, Peijia; Wang, Yanjing; Li, Chengjie; Wei, Lian-Bo

    2015-11-01

    The aim is to study the effects and underlying mechanisms of astragalus polysaccharide (APS) on the peroxide-induced injury in C2C12 myoblasts in vitro. Cell viability in the presence or absence of APS was detected by the methyl thiazolyl tetrazolium colorimetric assay. The autophagosomes were observed by electron microscopy to examine the influence of APS on autophagy caused by H2O2 in C2C12 cells, and the percentage of apoptosis cells was measured by flow cytometry. To further confirm the effect of H2O2 on C2C12 cells, the protein expression of LC3 and RARP, which are the markers of autophagy and apoptosis, respectively, was analyzed by Western blot, as well as the expression levels of p-p70S6K, p70S6K, Bcl-2, Bax, cyto-C, and Caspase-3, to reveal the underlying mechanisms. We observed multiple effects of APS on C2C12 functionality. APS treatment of C2C12 cells at 1 mg/mL reduced cell viability to less than 70 %, and analysis by electron microscopy revealed that APS also reduced the number of H2O2-induced autophagosome formation. Similarly, APS abated the H2O2-mediated increase in cell apoptosis, which was accompanied by the inhibition of LC3 II and RARP that are normally upregulated by H2O2. The expression of p-p70S6K and p70S6K, however, remained unchanged in C2C12 cells in the Control, H2O2 and H2O2 + APS groups. In addition, APS promoted the expression of protein Bcl-2 in H2O2-treated C2C12 cells, but did not change Bax, thus reducing the Bax/Bcl-2 ratio that in turn prevented the release of cytochrome c and the activation of caspase-3. APS inhibits the autophagy and apoptosis induced by peroxide injury in C2C12 myoblasts through two independent signaling pathways: the mTOR-independent pathway for the inhibition of autophagy, and the caspase-3-dependent pathway for the suppression of apoptosis.

  8. Monte Carlo reference data sets for imaging research: Executive summary of the report of AAPM Research Committee Task Group 195.

    PubMed

    Sechopoulos, Ioannis; Ali, Elsayed S M; Badal, Andreu; Badano, Aldo; Boone, John M; Kyprianou, Iacovos S; Mainegra-Hing, Ernesto; McMillan, Kyle L; McNitt-Gray, Michael F; Rogers, D W O; Samei, Ehsan; Turner, Adam C

    2015-10-01

    The use of Monte Carlo simulations in diagnostic medical imaging research is widespread due to its flexibility and ability to estimate quantities that are challenging to measure empirically. However, any new Monte Carlo simulation code needs to be validated before it can be used reliably. The type and degree of validation required depends on the goals of the research project, but, typically, such validation involves either comparison of simulation results to physical measurements or to previously published results obtained with established Monte Carlo codes. The former is complicated due to nuances of experimental conditions and uncertainty, while the latter is challenging due to typical graphical presentation and lack of simulation details in previous publications. In addition, entering the field of Monte Carlo simulations in general involves a steep learning curve. It is not a simple task to learn how to program and interpret a Monte Carlo simulation, even when using one of the publicly available code packages. This Task Group report provides a common reference for benchmarking Monte Carlo simulations across a range of Monte Carlo codes and simulation scenarios. In the report, all simulation conditions are provided for six different Monte Carlo simulation cases that involve common x-ray based imaging research areas. The results obtained for the six cases using four publicly available Monte Carlo software packages are included in tabular form. In addition to a full description of all simulation conditions and results, a discussion and comparison of results among the Monte Carlo packages and the lessons learned during the compilation of these results are included. This abridged version of the report includes only an introductory description of the six cases and a brief example of the results of one of the cases. This work provides an investigator the necessary information to benchmark his/her Monte Carlo simulation software against the reference cases included here

  9. Mitochondria-targeted dodecyltriphenylphosphonium (C12TPP) combats high-fat-diet-induced obesity in mice

    PubMed Central

    Kalinovich, A V; Mattsson, C L; Youssef, M R; Petrovic, N; Ost, M; Skulachev, V P; Shabalina, I G

    2016-01-01

    Background: A membrane-penetrating cation, dodecyltriphenylphosphonium (C12TPP), facilitates the recycling of fatty acids in the artificial lipid membrane and mitochondria. C12TPP can dissipate mitochondrial membrane potential and may affect total energy expenditure and body weight in animals and humans. Methods: We investigated the metabolic effects of C12TPP in isolated brown-fat mitochondria, brown adipocyte cultures and mice in vivo. Experimental approaches included the measurement of oxygen consumption, carbon dioxide production, western blotting, magnetic resonance imaging and bomb calorimetry. Results: In mice, C12TPP (50 μmol per (day•kg body weight)) in the drinking water significantly reduced body weight (12%, P<0.001) and body fat mass (24%, P<0.001) during the first 7 days of treatment. C12TPP did not affect water palatability and intake or the energy and lipid content in feces. The addition of C12TPP to isolated brown-fat mitochondria resulted in increased oxygen consumption. Three hours of pretreatment with C12TPP also increased oligomycin-insensitive oxygen consumption in brown adipocyte cultures (P<0.01). The effects of C12TPP on mitochondria, cells and mice were independent of uncoupling protein 1 (UCP1). However, C12TPP treatment increased the mitochondrial protein levels in the brown adipose tissue of both wild-type and UCP1-knockout mice. Pair-feeding revealed that one-third of the body weight loss in C12TPP-treated mice was due to reduced food intake. C12TPP treatment elevated the resting metabolic rate (RMR) by up to 18% (P<0.05) compared with pair-fed animals. C12TPP reduced the respiratory exchange ratio, indicating enhanced fatty acid oxidation in mice. Conclusions: C12TPP combats diet-induced obesity by reducing food intake, increasing the RMR and enhancing fatty acid oxidation. PMID:27534841

  10. Prevalence and Clinical Impact of Anaplasia in Childhood Rhabdomyosarcoma: A Report from the Soft Tissue Sarcoma Committee for the Children’s Oncology Group

    PubMed Central

    Qualman, Stephen; Lynch, James; Bridge, Julia; Parham, David; Teot, Lisa; Meyer, William; Pappo, Alberto

    2009-01-01

    Background Anapalsia is rare in childhood rhabdomyosarcoma and has not been included in the International Classification of Rhabdomyosarcoma (ICR). A recent review of cases from the Soft Tissue Sarcoma Committee of the Children’s Oncology Group (COG) suggests that anaplasia might be more common than previously reported and may impact clinical outcome. Materials and Methods The prevalence of anaplasia (focal or diffuse) was prospectively assessed in 546 eligible cases who were registered in an Intergroup Rhabdomyosarcoma Study Group (IRSG) or COG therapeutic trial from 1995–1998. The incidence of anaplasia in tumor samples and its impact in predicting clinical outcome was assessed. Results Overall 71 (13%) of all samples analyzed had anaplasia. Anaplasia was more common in patients with tumors in favorable sites and was less commonly seen in younger patients and in those with stage 2, 3 or clinical Group III disease. Regardless of its distribution (focal or diffuse), on univariate analysis the presence of anaplasia had a significant negative impact for both failure-free survival (FFS: 63% vs 77% at 5 years) and survival (S: 68% vs 82% at 5 years) in patients with embryonal rhabdomyosarcoma. This effect was most pronounced in children with intermediate risk disease. Using multivariate analysis, the hazard ratio was 1.6 for FFS (p=0.085) and 1.7 for overall survival (p=0.081). Anaplasia did not affect outcome in patients with alveolar tumors. Conclusion The incidence of anaplasia in rhabdomyosarcoma is higher than previously described and may be of prognostic significance in children with intermediate risk embryonal rhabdomyosarcoma. PMID:18985676

  11. Phase Behavior of Liquid Crystals Formed in [C12mim]CI/H2O and [C12mim]CI/Alcohols Systems

    NASA Astrophysics Data System (ADS)

    Pei, Mei-shan; Wu, Zhi-yan; Wang, Lu-yan; Wu, Xin-zhou; Tao, Xu-tang

    2009-10-01

    Phase behaviors of different binary systems involving 1-dodecyl-3-methylimidazolium chloride ([C12mim]Cl) and H2O, [C12mim]Cl and different alcohols (1-butanol, 1-pentanol, 1-hexanol and 1-octanol) are investigated at 25 °C. Hexagonal liquid crystal phase (H1) is identified in [C12mim]Cl/H2O system, and lamellar liquid-crystalline (Lα) phase is found in [C12mim]Cl/alcohols systems by using polarized optical microscopy and small-angle X-ray scattering techniques. The formation of such phases is considered as a synergetic result of the solvatophobic force and the hydrogen-bonded network comprising an imidazolium ring, chloride ion and water (or alcohols), which can be confirmed by Fourier transform infrared spectra. It is noticeable that in [C12mim]Cl/1-octanol system, the lattice spacings of lamellar phase increase with increasing C12mimCl concentration, which is opposite to the results of [C12mim]Cl/H2O system. This may result mainly from stronger static repulsion among hydrophilic headgroups of imidazolium salts arranged in the bilayers of lamellar structures. Further measurements by differential scanning calorimetry indicate that the lamellar phase is stable within a wide temperature range above room temperature. However, the lattice spacings decrease with the increase of temperature, which may be due to the softening of the hydrocarbon chain of [C12mim]Cl molecules. In different alcohols systems, it is found that the lamellar lyotropic liquid crystal structure is easier to be formed when the carbon chain length becomes longer.

  12. [Modification of pertussis vaccination schedule in Chile, immunization of special groups and control strategies: Commentary from the Consultive Committee of Immunizations of The Chilean Society of Infectious Diseases].

    PubMed

    Potin, Marcela; Cerda, Jaime; Contreras, Lily; Muñoz, Alma; Ripoll, Erna; Vergara, Rodrigo

    2012-06-01

    In Chile, an increased number of notifications of cases of whooping cough was detected at the beginning of October 2010, and maintained through 2012. Accumulated cases during 2011 were 2,581 (15.0 per 100,000), which is greater than the number of cases registered during the period 2008-2010 (2,460 cases). On the other hand, the local sanitary authority introduced a modification of pertussis vaccination schedule (starting 2012), which consists in the replacement of the second booster of pertussis vaccine (DTwP, administered to 4-year-old children) as well as diphtheria-tetanus toxoid (dT, administered to second grade scholars) for an acellular pertussis vaccine with reduced antigenic content (dTpa), which will be administrated to first grade scholars. The Consultive Committee of Immunizations considers that the modification is adequate, since it extends the age of protection, reducing at least in theory the infection in older scholars and adolescents -who are significant sources of transmission of Bordetella pertussis to infants- using an adequate vaccine formulation (acellular pertussis vaccine). The available evidence regarding vaccination in special groups (adolescents and adults, health-care workers and pregnant women) and cocooning strategy are commented.

  13. Structure and aggregation proclivity of C12E3 in aqueous solution

    NASA Astrophysics Data System (ADS)

    Zahariev, Ts.; Ivanova, A.; Velinova, M.; Tadjer, A.

    2013-01-01

    Olygo(ethylene glycol) alkyl ethers - CxEy are well known for their high surface activity and rich phase behavior. These substances exhibit some unusual aggregation characteristics in aqueous solution even at concentrations well below CMC attributed to the formation of pre-aggregates of small size, e.g., dimers. To verify this, a series of C12E3 dimers with initial geometries taken from coarse-grained molecular dynamics simulations is subject to geometry optimization with two quantum chemical methods: DFT and ONIOM in implicit water solvent. The resulting structures are classified into groups based on structural parameters. Their stability is assessed by relative and binding energy and rationalized in terms of molecular characteristics. All studied dimers are stable and various mutual alignments of the surfactants therein are feasible. The loss of surface area is outlined as the predominant stabilizing factor. Substantial number of the structures is suitable for further aggregation.

  14. 41 CFR 102-3.40 - What types of committees or groups are not covered by the Act and this part?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Sciences (NAS) or the National Academy of Public Administration (NAPA). Any committee created by NAS or NAPA in accordance with section 15 of the Act, except as otherwise covered by subpart E of this...

  15. New AGU mineral physics committee

    NASA Astrophysics Data System (ADS)

    Bell, Peter M.

    A new Committee on Mineral Physics consisting of Orson Anderson (chairman), Peter Bell, Raymond Jeanloz, Robert Lieberman, Murli Manghnani, Alexandra Navrotsky, Tom Shankland, Joseph E. Smith, and Donald Weidner has been approved by the AGU Executive Committee.The increasing number of research groups in an area that combines the study of mineral properties and solid state sciences (materials research) created the impetus for this new committee. At AGU meetings, mineral physics studies have been included in recent years in sessions of Volcanology, Petrology, and Geochemistry and sessions of Tectonophysics. A portion of the charter for the new committee includes arranging special sessions for mineral physics that would bridge the two sections.

  16. Pseudomonas aeruginosa biofilm-associated homoserine lactone C12 rapidly activates apoptosis in airway epithelia.

    PubMed

    Schwarzer, Christian; Fu, Zhu; Patanwala, Maria; Hum, Lauren; Lopez-Guzman, Mirielle; Illek, Beate; Kong, Weidong; Lynch, Susan V; Machen, Terry E

    2012-05-01

    Pseudomonas aeruginosa (PA) forms biofilms in lungs of cystic fibrosis (CF) patients, a process regulated by quorum-sensing molecules including N-(3-oxododecanoyl)-l-homoserine lactone (C12). C12 (10-100 µM) rapidly triggered events commonly associated with the intrinsic apoptotic pathway in JME (CF ΔF508CFTR, nasal surface) epithelial cells: depolarization of mitochondrial (mito) membrane potential (Δψ(mito)) and release of cytochrome C (cytoC) from mitos into cytosol and activation of caspases 3/7, 8 and 9. C12 also had novel effects on the endoplasmic reticulum (release of both Ca(2+) and ER-targeted GFP and oxidized contents into the cytosol). Effects began within 5 min and were complete in 1-2 h. C12 caused similar activation of caspases and release of cytoC from mitos in Calu-3 (wtCFTR, bronchial gland) cells, showing that C12-triggered responses occurred similarly in different airway epithelial types. C12 had nearly identical effects on three key aspects of the apoptosis response (caspase 3/7, depolarization of Δψ(mito) and reduction of redox potential in the ER) in JME and CFTR-corrected JME cells (adenoviral expression), showing that CFTR was likely not an important regulator of C12-triggered apoptosis in airway epithelia. Exposure of airway cultures to biofilms from PAO1wt caused depolarization of Δψ(mito) and increases in Ca(cyto) like 10-50 µM C12. In contrast, biofilms from PAO1ΔlasI (C12 deficient) had no effect, suggesting that C12 from P. aeruginosa biofilms may contribute to accumulation of apoptotic cells that cannot be cleared from CF lungs. A model to explain the effects of C12 is proposed. © 2012 Blackwell Publishing Ltd.

  17. Pseudomonas aeruginosa biofilm-associated homoserine lactone C12 rapidly activates apoptosis in airway epithelia

    PubMed Central

    Schwarzer, Christian; Fu, Zhu; Patanwala, Maria; Hum, Lauren; Lopez-Guzman, Mirielle; Illek, Beate; Kong, Weidong; Lynch, Susan V.; Machen, Terry E.

    2014-01-01

    Pseudomonas aeruginosa (PA) forms biofilms in lungs of cystic fibrosis CF) patients, a process regulated by quorum sensing molecules including N-(3-oxododecanoyl)-L-homoserine lactone, C12. C12 (10–100 μM) rapidly triggered events commonly associated with the intrinsic apoptotic pathway in JME (CFΔF508CFTR, nasal surface) epithelial cells: depolarization of mitochondrial (mito) membrane potential (Δψmito) and release of cytochrome C (cytoC) from mitos into cytosol and activation of caspases 3/7, 8 and 9. C12 also had novel effects on the endoplasmic reticulum (release of both Ca2+ and ER-targeted GFP and oxidized contents into the cytosol). Effects began within 5 minutes and were complete in 1–2 hrs. C12 caused similar activation of caspases and release of cytoC from mitos in Calu-3 (wtCFTR, bronchial gland) cells, showing that C12-triggered responses occurred similarly in different airway epithelial types. C12 had nearly identical effects on three key aspects of the apoptosis response (caspase 3/7, depolarization of Δψmito and reduction of redox potential in the ER) in JME and CFTR-corrected JME cells (adenoviral expression), showing that CFTR was likely not an important regulator of C12-triggered apoptosis in airway epithelia. Exposure of airway cultures to biofilms from PAO1wt caused depolarization of Δψmito and increases in Cacyto like 10–50 μM C12. In contrast, biofilms from PAO1ΔlasI (C12 deficient) had no effect, suggesting that C12 from P. aeruginosa biofilms may contribute to accumulation of apoptotic cells that cannot be cleared from CF lungs. A model to explain the effects of C12 is proposed. PMID:22233488

  18. Use of image registration and fusion algorithms and techniques in radiotherapy: Report of the AAPM Radiation Therapy Committee Task Group No. 132.

    PubMed

    Brock, Kristy K; Mutic, Sasa; McNutt, Todd R; Li, Hua; Kessler, Marc L

    2017-07-01

    Image registration and fusion algorithms exist in almost every software system that creates or uses images in radiotherapy. Most treatment planning systems support some form of image registration and fusion to allow the use of multimodality and time-series image data and even anatomical atlases to assist in target volume and normal tissue delineation. Treatment delivery systems perform registration and fusion between the planning images and the in-room images acquired during the treatment to assist patient positioning. Advanced applications are beginning to support daily dose assessment and enable adaptive radiotherapy using image registration and fusion to propagate contours and accumulate dose between image data taken over the course of therapy to provide up-to-date estimates of anatomical changes and delivered dose. This information aids in the detection of anatomical and functional changes that might elicit changes in the treatment plan or prescription. As the output of the image registration process is always used as the input of another process for planning or delivery, it is important to understand and communicate the uncertainty associated with the software in general and the result of a specific registration. Unfortunately, there is no standard mathematical formalism to perform this for real-world situations where noise, distortion, and complex anatomical variations can occur. Validation of the software systems performance is also complicated by the lack of documentation available from commercial systems leading to use of these systems in undesirable 'black-box' fashion. In view of this situation and the central role that image registration and fusion play in treatment planning and delivery, the Therapy Physics Committee of the American Association of Physicists in Medicine commissioned Task Group 132 to review current approaches and solutions for image registration (both rigid and deformable) in radiotherapy and to provide recommendations for quality

  19. 40 CFR 721.9285 - Reaction products of formalin (37%) with amine C12.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Reaction products of formalin (37... Specific Chemical Substances § 721.9285 Reaction products of formalin (37%) with amine C12. (a) Chemical... as reaction products of formalin (37%) with amine C12 (PMN P-95-535) is subject to reporting...

  20. 40 CFR 721.9285 - Reaction products of formalin (37%) with amine C12.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Reaction products of formalin (37... Specific Chemical Substances § 721.9285 Reaction products of formalin (37%) with amine C12. (a) Chemical... as reaction products of formalin (37%) with amine C12 (PMN P-95-535) is subject to reporting...

  1. 40 CFR 721.9285 - Reaction products of formalin (37%) with amine C12.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Reaction products of formalin (37... Specific Chemical Substances § 721.9285 Reaction products of formalin (37%) with amine C12. (a) Chemical... as reaction products of formalin (37%) with amine C12 (PMN P-95-535) is subject to reporting...

  2. 40 CFR 721.9285 - Reaction products of formalin (37%) with amine C12.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Reaction products of formalin (37... Specific Chemical Substances § 721.9285 Reaction products of formalin (37%) with amine C12. (a) Chemical... as reaction products of formalin (37%) with amine C12 (PMN P-95-535) is subject to reporting...

  3. Developing Effective and Efficient Local Committees. Revised.

    ERIC Educational Resources Information Center

    Dyer, Delwyn A.; Williams, Oscar M.

    Local community leaders should examine the principles involved in making committees an effective and efficient tool in group operations. A committee assists the parent organization in developing programs, carrying out business, establishing policies, executing affairs, and evaluating an organization's effectiveness. Committees have many social,…

  4. [Health evaluation of trichloroethylene in indoor air : communication from the German ad-hoc working group on indoor guidelines of the Indoor Air Hygiene Committee and of the states' supreme health authorities].

    PubMed

    2015-07-01

    In the European Hazardous Substances Regulation No 1272/2008 trichloroethylene has been classified as a probable human carcinogen and a suspected mutagen. According to several Committees (German Committee on Hazardous Substances, European Scientific Committee on Occupational Exposure Limits, European Chemicals Agency´s Committee for Risk Assessment (ECHA-RAC)) concentrations of trichloroethylene cytotoxic to renal tubuli may increase the risk to develop renal cancer. At non-cytotoxic concentrations of trichloroethylene a much lower cancer risk may be assumed. Therefore, evaluating the cancer risk to the public following inhalation of trichloroethylene ECHA-RAC has assumed a sublinear exposure-response relationship for carcinogenicity of trichloroethylene. Specifically, ECHA-RAC assessed a cancer risk of 6.4 × 10(- 5) (mg/m(3))(- 1) following life time exposure to trichloroethylene below a NOAEC for renal cytotoxicity of 6 mg trichloroethylene/m(3). Further evaluation yields a life-time risk of 10(- 6) corresponding to 0.02 mg trichloroethylene/m(3). This concentration is well above the reference (e.g. background) concentration of trichloroethylene in indoor air. Consequently the Ad-hoc Working Group on Indoor Guidelines recommends 0.02 mg trichloroethylene/m(3) as a risk-related guideline for indoor air. Measures to reduce exposure are considered inappropriate at concentrations below this guideline.

  5. 76 FR 621 - Commercial Space Transportation Advisory Committee-Public Teleconference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-05

    ... Space Transportation Advisory Committee (COMSTAC). The teleconference will take place on Thursday... Working Groups. The Committee will examine the current Working Groups and discuss whether it should make...

  6. Structures and electronic properties of C12Si8X8 (X = H, F, and Cl)

    NASA Astrophysics Data System (ADS)

    Naderi, F.

    2016-07-01

    The structural stabilities and electronic properties of C12Si8X8 where X = H, F, and Cl are probed on the basis of density functional theory at the B3LYP/6-311++G**//B3LYP/6-31+G* level. Vibrational frequency calculations show that all the systems are true minima. The infrared spectra of the most stable C12Si8X8 molecules are simulated to assist further experimental characterization. The functionalized structures and energy gaps between the highest occupied molecular orbital, HOMO, and the lowest unoccupied molecular orbital, LUMO, have been systematically investigated. It seems that C12Si8H8 has more stability against electronic excitations via increasing the HOMO-LUMO gap comparing with C12Si8Cl8 and C12Si8F8. High charge transfer on the surfaces of our stable compounds, provokes further investigations on their possible application for hydrogen storage. The addition reaction energies of C12Si8X8 are high exothermic, and C12Si8F8 is more thermodynamically accessible.

  7. Infrared Spectroscopic Study of C12/02 Co-Adsorption on Al(111)

    DTIC Science & Technology

    1988-08-01

    adsorbed 0 and Cl leading to an enhancement of 6 the Al -O dynamic dipole moment. The situation is more complicated in other exposure regimes [e.g., Figs...Include Security Classification) Unclassified Technical Report No. 6 . "Infrared Spectroscopic Study of C12/02 Co-Adsorption on Al (111). 12 PERSONAL...IRRARS) has been used to observe the effects of C12 (02 ) pre-adsorption on the vibrational modes of 0 (Cl) formed by exposure of Al (111) to 0 ’ (C12

  8. First principles centroid molecular dynamics simulation of hydride in nanoporous C12A7:H-

    NASA Astrophysics Data System (ADS)

    Ikeda, Takashi

    2017-05-01

    Hydrides in nanoporous [Ca24Al28O64]4+(H-)4 (C12A7:H-) were investigated via first principles centroid molecular dynamics (CMD). The quality of our CMD simulations was assessed by examining the temperature dependence of the distribution of hydrides in the cages constituting the C12A7 framework. The vibrational states of C12A7:H- were analyzed by using the trajectories of the centroids generated in our CMD simulations. We find that the rattling motions of H- and D- behave qualitatively differently, resulting in non-trivial isotope effects, which are suggested to be detectable by using infrared and Raman spectroscopy.

  9. Update of technical coordinating committee activities

    SciTech Connect

    Alvarado, R.A.

    1995-12-31

    The Technical Coordinating Committee has its origins in the earliest days of implementing the Low-Level Radioactive Waste Policy Act. Between 1982 and 1985, individuals in several of the states felt that coordination among the states would be beneficial to all by affording states a cost-effective method for sharing ideas, discussing alternatives, and presenting solutions to common problems. At the current time, the committee comprises members from each of the sited states. Various compacts, federal agencies, and industry groups participate in committee activities. The Low-Level Management Program provides support for the committee through the provision of logistical support and limited manpower allocation. Activities of the committee have recently focused on waste treatment and minimization technologies. The committee also has worked diligently to see the review of the 3RSTAT computer code completed. The committee has taken a position on various regulatory proposals the past year. The committee expects to continue its work until new sites are brought online.

  10. The Development Committee. Effective Committees. Board Basics.

    ERIC Educational Resources Information Center

    Evans, Gary

    1997-01-01

    A college or university governing board's development committee (or foundation board in a public institution) has both oversight of and broad responsibility for institutional fund-raising activities. Committee members have an additional role that colleagues on other committees do not--active participation in fund-raising. The committee sets goals,…

  11. Fatty acid composition of porcine cumulus oocyte complexes (COC) during maturation: effect of the lipid modulators trans-10, cis-12 conjugated linoleic acid (t10,c12 CLA) and forskolin.

    PubMed

    Prates, E G; Alves, S P; Marques, C C; Baptista, M C; Horta, A E M; Bessa, R J B; Pereira, R M

    2013-05-01

    The effect of maturation and of two lipid modulators supplementation along in vitro maturation (IVM) on fatty acid (FA) and dimethylacetal (DMA) composition of porcine cumulus oocyte complexes (COC) were studied. Abattoir-derived immature COC were analyzed for FA and DMA or submitted to IVM as follows: control group; t10,c12 CLA group, t10,c12 CLA supplementation for 44 h; Forskolin group, forskolin supplementation during the initial 2 h; t10,c12 CLA + forskolin group, t10,c12 CLA for 44 h and forskolin for just 2h. Each experimental group had five replicates. FA analysis of oocytes, cumulus cells (CC), follicular fluid, and culture media were performed by gas-liquid chromatography. Oocytes and their CC had different FA composition. Oocytes were richer in saturated FA (SFA) preferentially maintaining their FA profile during maturation. Mature CC had the highest polyunsaturated FA (PUFA) content. Five individual and total SFA, and monounsaturated FA (MUFA), notably oleic acid (c9-18:1), percentages were lower (P ≤ 0.023) in mature than in immature CC. t10,c12 CLA was accumulated by COC from t10,c12 CLA and t10,c12 CLA + forskolin groups, mostly in CC where MUFA and an eicosatrienoic isomer decreased (P ≤ 0.043). Nevertheless, PUFA or FA and DMA total content were not affected. Arachidonic acid was reduced in t10,c12 CLA + forskolin CC and hexadecanal-DMA-16:0 in t10,c12 CLA CC. Forskolin alone increased (P ≤ 0.043) c9-18:1 in oocytes. In conclusion, maturation process clearly changed porcine COC FA and DMA profiles, mostly of CC, also more susceptible to modifications induced by t10,c12 CLA. This possibility of manipulating COC lipid composition during IVM could be used to improve oocyte quality/cryopreservation efficiency.

  12. CRF Type 2 Receptors Mediate the Metabolic Effects of Ghrelin in C2C12 cells

    PubMed Central

    Gershon, Eran; Vale, Wylie W

    2014-01-01

    Objective Ghrelin is known to regulate appetite control and cellular metabolism. The Corticotropin-Releasing Factor (CRF) family is also known to regulate energy balance. In this study, we investigated the links between ghrelin and the CRF family in C2C12 cells, a mouse myoblast cell line. Design and methods C2C12 cells were treated with ghrelin in the presence or absence of CRF receptor antagonists and then subjected to different metabolic analyses. Results Ghrelin enhanced glucose uptake by C2C12 cells, induced GLUT4 translocation to the cell surface and decreased RBP4 expression. A CRF-R2 selective antagonist, anti-sauvagine-30, blocked ghrelin-induced glucose uptake, Ghrelin upregulated CRF-R2 but not CRF-R1 levels. Moreover, ghrelin-treated C2C12 cells displayed a cAMP and pERK activation in response to Ucn3, a CRF-R2 specific ligand, but not in response to CRF or stressin, CRF-R1 specific ligands. Ghrelin also induced UCP2 and UCP3 expression, which were blocked by anti-sauvagine-30. Ghrelin did not induce fatty acids uptake by C2C12 cells or ACC expression. Even though C2C12 cells clearly exhibited responses to ghrelin, the known ghrelin receptor, GHSR1a, was not detectable in C2C12 cells. Conclusion Our results suggest that, ghrelin plays a role in regulating muscle glucose and, raise the possibility that suppression of the CRF-R2 pathway might provide benefits in high ghrelin states. PMID:23804489

  13. 7 CFR 610.23 - State Technical Committee meetings.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... governing the operation of State Technical Committees and Local Working Groups in its directive system. The..., organizing, and effectively utilizing State Technical Committees and Local Working Groups; direction on publication of State Technical Committee and Local Working Group meeting notices and agendas; State...

  14. Medical ethics committees in Hungary.

    PubMed

    Blasszauer, B

    1991-01-01

    Hungarian medical ethics committees were established at the end of the 1950s. They came into being on the Communist Party's initiative. They could hardly be called "interdisciplinary" since their membership was made up of high-ranking physicians and a few head nurses. Their main task was to counter the practice of "tipping." Medical ethics and "tipping" were practically synonymous. These committees did not confront or try to resolve ethical problems concerning such issues as patient rights, informed consent, refusal of treatment, human experimentation, abortion, etc. These committees - whether it is believable or not - belonged to the Physicians Health Workers Trade Union. They were under the guidance and supervision of this social organization. The public was excluded from their meetings, and the committees' duty was to follow the health laws which were supposed to have given excellent ethical guidance. Even in a textbook on medical ethics used at one of the medical universities, written by a psychiatrist, the health laws were presented and explained back and forth. Of the 88 pages only 23 dealt with morals in general and the Hippocratic tradition. The Hungarian National Health Service as well as its medical ethics committees are similar in many respects to the Soviet and Eastern European countries' health care system and ethics committees. Since radical changes have taken place in these so-called "former" communist countries, it can only be hoped that these committees will eventually develop into groups who will deal directly with the moral questions or medicine and health care.

  15. Determination of (C-12)/(C-13) in the interstellar medium toward Zeta Ophiuchi and Xi Persei

    NASA Technical Reports Server (NTRS)

    Hawkins, Isabel; Craig, Nahide; Meyer, David M.

    1993-01-01

    The Lick Observatory Reticon (LOR) data presented in Hawkins et al. (1985) are reanalyzed in order to determine the cause of the significant discrepancy in (C-12)/(C-13) isotope ratios determined on the basis of observations of this isotope in the interstellar medium toward Zeta Ophiuchi and Xi Persei. A new set of Lick Observatory observations toward Zeta Oph are obtained using a CCD detector. The KPNO coude feed echelle spectrograph and a CCD detector are used to carry out high-resolution, high SNR ratio observations toward Zeta Oph and Xi Per. The LOR results for Zeta Oph are uncertain, ranging to 40 to 60, owing to the error in continuum placement. The KPNO results toward Zeta Oph are (C-12)H/(C-13)H(+)(0, 0) = 63 +/- 8, (C-12)H(+)/(C-13)H(+)(1, 0) = 67 +/- 19, and (C-12)N/(C-13)N = 100 + 88/- 33. Toward Xi Per, the KPNO measurements yield (C-12)H(+)/(C-13)H(+) = 49 +/- 15 from the 4232-A band, and a lower limit of 45 from the 3957-A observations.

  16. DRAGON, a GPI-anchored membrane protein, inhibits BMP signaling in C2C12 myoblasts.

    PubMed

    Kanomata, Kazuhiro; Kokabu, Shoichiro; Nojima, Junya; Fukuda, Toru; Katagiri, Takenobu

    2009-06-01

    Bone morphogenetic proteins (BMPs) induce osteoblastic differentiation of myoblasts via binding to cell surface receptors. Repulsive guidance molecules (RGMs) have been identified as BMP co-receptors. We report here that DRAGON/RGMb, a member of the RGM family, suppressed BMP signaling in C2C12 myoblasts via a novel mechanism. All RGMs were expressed in C2C12 cells that were differentiated into myocytes and osteoblastic cells, but RGMc was not detected in immature cells. In C2C12 cells, only DRAGON suppressed ALP and Id1 promoter activities induced by BMP-4 or by constitutively activated BMP type I receptors. This inhibition by DRAGON was dependent on the secretory form of the von Willbrand factor type D domain. DRAGON even suppressed BMP signaling induced by constitutively activated Smad1. Over-expression of neogenin did not alter the inhibitory capacity of DRAGON. Taken together, these findings indicate that DRAGON may be an inhibitor of BMP signaling in C2C12 myoblasts. We also suggest that a novel molecule(s) expressed on the cell membrane may mediate the signal transduction of DRAGON in order to suppress BMP signaling in C2C12 myoblasts.

  17. Determination of (C-12)/(C-13) in the interstellar medium toward Zeta Ophiuchi and Xi Persei

    NASA Technical Reports Server (NTRS)

    Hawkins, Isabel; Craig, Nahide; Meyer, David M.

    1993-01-01

    The Lick Observatory Reticon (LOR) data presented in Hawkins et al. (1985) are reanalyzed in order to determine the cause of the significant discrepancy in (C-12)/(C-13) isotope ratios determined on the basis of observations of this isotope in the interstellar medium toward Zeta Ophiuchi and Xi Persei. A new set of Lick Observatory observations toward Zeta Oph are obtained using a CCD detector. The KPNO coude feed echelle spectrograph and a CCD detector are used to carry out high-resolution, high SNR ratio observations toward Zeta Oph and Xi Per. The LOR results for Zeta Oph are uncertain, ranging to 40 to 60, owing to the error in continuum placement. The KPNO results toward Zeta Oph are (C-12)H/(C-13)H(+)(0, 0) = 63 +/- 8, (C-12)H(+)/(C-13)H(+)(1, 0) = 67 +/- 19, and (C-12)N/(C-13)N = 100 + 88/- 33. Toward Xi Per, the KPNO measurements yield (C-12)H(+)/(C-13)H(+) = 49 +/- 15 from the 4232-A band, and a lower limit of 45 from the 3957-A observations.

  18. Ruminant Methane δ (13C/12C) - Values: Relation to Atmospheric Methane

    NASA Astrophysics Data System (ADS)

    Rust, Fleet

    1981-03-01

    The δ (13C/12C) - values of methane produced by fistulated steers, dairy cattle, and wethers, and dairy and beef cattle herds show a bimodal distribution that appears to be correlated with the plant type (C3 or C4, that is, producing either a three- or a four-carbon acid in the first step of photosynthesis) consumed by the animals. These results indicate that cattle and sheep, on a global basis, release methane with an average δ (13C/12C) value of -60 and -63 per mil, respectively. Together they are a source of atmospheric methane whose δ (13C/12C) is similar to published values for marsh gas and cannot explain the 20 per mil higher values for atmospheric methane.

  19. Anomalous C-12H4:C-13H4 strengths in 3 ny sub 3

    NASA Technical Reports Server (NTRS)

    Brault, J. W.; Fox, K.; Jennings, D. E.; Margolis, J. S.

    1981-01-01

    The C-12:C-13 ratios in the atmospheres of the outer planets are important parameters with which to test models for isotope formation and fractionation during the early history of the solar system, yielding insight into processes occurring in the primitive solar nebula. The 3 ny sub 3 spectra of methane were obtained using the Kitt Peak Solar Fourier Transform Spectrometer and a 6 m White cell. The experimental results are summarized in a table. The strength ratios for the R-branch of 3 ny sub 3 exhibit strong anomalies, with C-13H4 lines consistently weaker than their C-12H4 counterparts. The C-12:C-13 abundance ratios for the atmospheres of Jupiter and Saturn have been redetermined using the 3 ny sub 3 line-strength ratios measured.

  20. Fusion measurements of 12C+12C at energies of astrophysical interest

    NASA Astrophysics Data System (ADS)

    Santiago-Gonzalez, D.; Jiang, C. L.; Rehm, K. E.; Alcorta, M.; Almaraz-Calderon, S.; Avila, M. L.; Ayangeakaa, A. D.; Back, B. B.; Bourgin, D.; Bucher, B.; Carpenter, M. P.; Courtin, S.; David, H. M.; Deibel, C. M.; Dickerson, C.; DiGiovine, B.; Fang, X.; Greene, J. P.; Haas, F.; Henderson, D. J.; Janssens, R. V. F.; Jenkins, D.; Lai, J.; Lauritsen, T.; Lefebvre-Schuhl, A.; Montanari, D.; Pardo, R. C.; Paul, M.; Seweryniak, D.; Tang, X. D.; Ugalde, C.; Zhu, S.

    2016-05-01

    The cross section of the 12C+12C fusion reaction at low energies is of paramount importance for models of stellar nucleosynthesis in different astrophysical scenarios, such as Type Ia supernovae and Xray superbursts, where this reaction is a primary route for the production of heavier elements. In a series of experiments performed at Argonne National Laboratory, using Gammasphere and an array of Silicon detectors, measurements of the fusion cross section of 12C+12C were successfully carried out with the γ and charged-particle coincidence technique in the center-of-mass energy range of 3-5 MeV. These were the first background-free fusion cross section measurements for 12C+12C at energies of astrophysical interest. Our results are consistent with previous measurements in the high-energy region; however, our lowest energy measurement indicates a fusion cross section slightly lower than those obtained with other techniques.

  1. Search for 12 C + 12 C clustering in 24 M g ground state

    NASA Astrophysics Data System (ADS)

    Joshi, B. N.; Jain, Arun K.; Biswas, D. C.; John, B. V.; Gupta, Y. K.; Danu, L. S.; Vind, R. P.; Prajapati, G. K.; Mukhopadhyay, S.; Saxena, A.

    2017-02-01

    In the backdrop of many models, the heavy cluster structure of the ground state of 24Mg has been probed experimentally for the first time using the heavy cluster knockout reaction 24Mg(12C,212C)12C in the quasifree scattering kinematic domain. In the (12C,212C) reaction, the direct 12C-knockout cross-section was found to be very small. Finite-range knockout theory predictions were much larger for (12C,212C) reaction, indicating a very small 12C-12C clustering in 24Mg(g.s.). Our present results contradict most of the proposed heavy cluster (12C+12C) structure models for the ground state of 24Mg.

  2. C-13/C-12 of atmospheric CO2 in the Amazon basin - Forest and river sources

    NASA Technical Reports Server (NTRS)

    Quay, Paul; King, Stagg; Wilbur, Dave; Richey, Jeffrey; Wofsy, Steven

    1989-01-01

    Results are presented of measurements of the CO2 concentrations and C-13/C-12 ratios in CO2 in air samples collected from within the Amazonian rain forest and over the Amazon river between 1982 and 1987. Results indicate the presence of a diurnal cycle in the CO2 concentration and the C-13/C-12 ratio. It was found that the CO2 input to air in the forest was derived from the soil respiration, and the CO2 input to air over the Amazon river was derived from the degassing of CO2 from the river. It was also found that plants growing at heights lower than 7 m assimilate soil-derived CO2 with a low C-13/C-12 ratio.

  3. REPORT AND RECOMMENDATIONS OF THE WORKING GROUP ON ASBESTOS AND CANCER: Convened under the auspices of the Geographical Pathology Committee of the International Union against Cancer (U.I.C.C.)

    PubMed Central

    1965-01-01

    The Geographical Pathology Committee of the International Union Against Cancer (U.I.C.C.) convened a Working Group on 22 to 23 October, 1964 in New York to discuss evidence of an association between exposure to asbestos dust and cancer. Forty delegates from eight countries attended, and separate panels on epidemiology, pathology and experimental pathology, and physics and chemistry met and, at the final session, under the Chairmanship of Dr. Harold Stewart (U.S.A) prepared the Report and Recommendations published below.

  4. Reassessment of the 13C/12C and 14C/12C isotopic fractionation ratio and its impact on high-precision radiocarbon dating

    NASA Astrophysics Data System (ADS)

    Fahrni, Simon M.; Southon, John R.; Santos, Guaciara M.; Palstra, Sanne W. L.; Meijer, Harro A. J.; Xu, Xiaomei

    2017-09-01

    The vast majority of radiocarbon measurement results (14C/12C isotopic ratios or sample activities) are corrected for isotopic fractionation processes (measured as 13C/12C isotopic ratios) that occur in nature, in sample preparation and measurement. In 1954 Harmon Craig suggested a value of 2.0 for the fractionation ratio b that is used to correct 14C/12C ratios for shifts in the 13C/12C ratios and this value has been applied by the radiocarbon community ever since. While theoretical considerations suggest moderate deviations of b from 2.0, some measurements have suggested larger differences (e.g. b = 2.3, measured by Saliège and Fontes in 1984). With the high precision attained in radiocarbon measurements today (±2‰), even a relatively small deviation of b from 2.0 can impact the accuracy of radiocarbon data, and it is, therefore, of interest to re-evaluate the fractionation corrections. In the present study, the fractionation ratio b was determined by independent experiments on the chemical reduction of carbon dioxide (CO2) to elemental carbon (graphitization reaction) and on the photosynthetic uptake of CO2 by C3 and C4 plants. The results yielded b = 1.882 ± 0.019 for the reduction of CO2 to solid graphite and b = 1.953 ± 0.025 for the weighted mean of measurements involving C3 and C4 photosynthesis pathways. In addition, the analysis of over 9600 full-sized OX-I and OX-II normalizing standards measured between 2002 and 2012 confirms b values lower than 2.0. The obtained values are in good agreement with quantum mechanical estimates of the equilibrium fractionation and classic kinetic fractionation as well as with results from other light three-isotope systems (oxygen, magnesium, silicon and sulfur). While the value of the fractionation ratio varies with the relative importance of kinetic and equilibrium fractionation, the values obtained in the present study cluster around b = 1.9. Our findings suggest that a significant fraction of all samples

  5. Executive committee

    NASA Astrophysics Data System (ADS)

    Xiao, Guoqing; Cai, Xiaohong; Ding, Dajun; Ma, Xinwen; Zhao, Yongtao

    2014-04-01

    ChairVice Chair Toshiyuki AzumaRoberto Rivarola Atomic, Molecular & Optical Physics LabUniversidad Nacional de Rosario and Advanced Science InstituteInstituto de Fisica Rosario RIKEN, JapanRosario, Argentina SecretaryMembers Dominique VernhetJoachim Burgdörfer, Austria Institut des NanoSciences de Paris Birgit Lohmann, Australia Université Pierre et Marie Curie Hossein Sadeghpour, USA Paris, FranceThomas Stöhlker, Germany Past ChairJim McCann, UK Barry DunningGuoqing Xiao, China Physics & AstronomyXiaohong Cai, China Rice University, HoustonXinwen Ma, China Texas, USAYongtao Zhao, China TreasurerFernando Martin, Spain Henrik CederquistLuis Mendez, Spain Alba Nova University CenterAnatoli Kheifets, Australia Stockholm University Stockholm, Sweden Details of the general committee are available in the PDF

  6. Conference Committees

    NASA Astrophysics Data System (ADS)

    2015-11-01

    Scientific Committee Silvia Arrese-Igor Irigoyen (CFM, CSIC - UPV/EHU, Donostia), Javier Campo (ICMA-CSIC, Zaragoza), Carlos Frontera (ICMAB-CSIC, Barcelona), Victoria García Sakai (ISIS, Chilton), Cristina Gómez-Polo (UPNa, Pamplona), Miguel Ángel González (ILL, Grenoble), Pedro Gorría (Universidad Oviedo), Jon Gutiérrez Echevarría (EHU/UPV, Bilbao), J. Iñaki Pérez Landazábal (UPNa, Pamplona), Vicente Recarte (UPNa, Pamplona), Jesús Ruíz Hervías (UPM, Madrid), Vicente Sánchez-Alarcos (UPNa, Pamplona), Antonio Urbina (UPC, Cartagena) Organizing Committee J. Iñaki Pérez Landazábal (Co-Chair), Vicente Recarte ( Co-Chair), Cristina Gómez-Polo, Silvia Larumbe Abuin, Vicente Sánchez-Alarcos Editors of the Proceedings J. Iñaki Pérez Landazábal, Vicente Recarte Plenary speakers Charles Simon (Institut Laue-Langevin, Grenoble, France), Miguel Angel Alario Franco (Universidad Complutense, Madrid, Spain), Dieter Richter (Jülich Centre for Neutron Science, Jülich, Germany), James Yeck (European Spallation Source, Lund, Sweden) Invited speakers Manu Barandiarán (BCMaterials & EHU/UPV), Arantxa Arbe (MFC, CSIC- UPV/EHU), José Luis Martínez (Consorcio ESS-Bilbao), Marta Castellote, IETcc-CSIC), Josep Lluis Tamarit (UPC), Diego Alba-Venero (ISIS), Elizabeth Castillo (CIC Energigune), Josu M. Igartua (EHU/UPV), Antonio Dos Santos (UPM), Alex Masalles (Museu Nacional d'Art de Catalunya), José Abad (UPCT), Claudia Mondelli (ILL), Oscar Fabelo (ILL), Aurora Nogales (IEM-CSIC), Jesús Rodríguez (UC), Gerardo

  7. Legal briefing: Healthcare ethics committees.

    PubMed

    Pope, Thaddeus Mason

    2011-01-01

    This issue's "Legal Briefing" column covers recent legal developments involving institutional healthcare ethics committees. This topic has been the subject of recent articles in JCE. Healthcare ethics committees have also recently been the subject of significant public policy attention. Disturbingly, Bobby Schindler and others have described ethics committees as "death panels." But most of the recent attention has been positive. Over the past several months, legislatures and courts have expanded the use of ethics committees and clarified their roles concerning both end-of-life treatment and other issues. These developments are usefully grouped into the following eight categories: 1. Existence and availability. 2. Membership and composition. 3. Operating procedures. 4. Advisory roles. 5. Decision-making and gate-keeping roles. 6. Confidentiality. 7. Immunity. 8. Litigation and court cases.

  8. Autophagic flux data in differentiated C2C12 myotubes following exposure to acetylcholine and caffeine.

    PubMed

    Bloemberg, Darin; Quadrilatero, Joe

    2016-06-01

    The C2C12 line of mouse myoblasts is a useful cell culture model in which to conduct in vitro analyses related to skeletal muscle. Here we present data regarding the autophagic response induced by two chemicals known to influence calcium release and contraction in skeletal muscles and C2C12 cells: acetylcholine and caffeine. More specifically, by concurrently administering acetylcholine or caffeine along with chloroquine to differentiated myotubes for various amounts of time and assessing the protein expression of LC3 and p62, we report data on the relative level of autophagic flux induced by these two calcium- and contraction-regulating chemicals.

  9. Ribbon structure stabilized by C10 and C12 turns in αγ hybrid peptide.

    PubMed

    Wani, Naiem Ahmad; Kant, Rajni; Gupta, Vivek Kumar; Aravinda, Subrayashastry; Rai, Rajkishor

    2016-04-01

    The present study describes the synthesis and crystallographic analysis of αγ hybrid peptides, Boc-Gpn-L-Pro-NHMe (1), Boc-Aib-Gpn-L-Pro-NHMe (2), and Boc-L-Pro-Aib-Gpn-L-Pro-NHMe (3). Peptides 1 and 2 adopt expanded 12-membered (C12 ) helical turn over γα segment. Peptide 3 promotes the ribbon structure stabilized by type II β-turn (C10 ) followed by the expanded C12 helical γα turn. Both right-handed and left-handed helical conformations for Aib residue are observed in peptides 2 and 3, respectively.

  10. Effect of mitochondrial fission inhibition on C2C12 differentiation

    PubMed Central

    Bloemberg, Darin; Quadrilatero, Joe

    2016-01-01

    The differentiation of skeletal muscle is commonly examined in cell culture using the C2C12 line of mouse skeletal myoblasts. This process shares many similarities with that which occurs during embryonic development, such as the transient activation of caspases. Here, we examined the effect of inhibiting mitochondrial fission, using mdivi-1, on the ability of C2C12 cells to terminally differentiate. This was performed using immunofluorescent identification of cell morphology and myosin expression, as well as immunoblotting for markers of muscle differentiation. Furthermore, the effect of mdivi-1 administration on activation of caspase-2 and -3 was assessed using spectrofluorometric measurement of specific enzyme activity. PMID:27054170

  11. 40 CFR 180.1068 - C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false C12-C18 fatty acid potassium salts... RESIDUES IN FOOD Exemptions From Tolerances § 180.1068 C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance. C12-C18 fatty acids (saturated and unsaturated) potassium salts are...

  12. 40 CFR 180.1068 - C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false C12-C18 fatty acid potassium salts... RESIDUES IN FOOD Exemptions From Tolerances § 180.1068 C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance. C12-C18 fatty acids (saturated and unsaturated) potassium salts are...

  13. 40 CFR 180.1068 - C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false C12-C18 fatty acid potassium salts... RESIDUES IN FOOD Exemptions From Tolerances § 180.1068 C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance. C12-C18 fatty acids (saturated and unsaturated) potassium salts are...

  14. 40 CFR 180.1068 - C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false C12-C18 fatty acid potassium salts... RESIDUES IN FOOD Exemptions From Tolerances § 180.1068 C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance. C12-C18 fatty acids (saturated and unsaturated) potassium salts are...

  15. 40 CFR 180.1068 - C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false C12-C18 fatty acid potassium salts... RESIDUES IN FOOD Exemptions From Tolerances § 180.1068 C12-C18 fatty acid potassium salts; exemption from the requirement of a tolerance. C12-C18 fatty acids (saturated and unsaturated) potassium salts are...

  16. The Investment Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Yoder, Jay A.

    2011-01-01

    This publication is part of an AGB series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices included in this text support the objectives of board committees:…

  17. The Investment Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Yoder, Jay A.

    2011-01-01

    This publication is part of an AGB series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices included in this text support the objectives of board committees:…

  18. OCLC and Its Advisory Committees.

    ERIC Educational Resources Information Center

    Baker, Shirley K.

    1998-01-01

    Describes the Online Computer Library Center (OCLC) advisory committees in terms of research, public, college and university, and special libraries. All four of the type-of-library advisory groups work to shape OCLC policies and programs, according to the particular needs of each group. OCLC's financial and programmatic success depends upon…

  19. 78 FR 64202 - Meeting To Discuss Lessons Learned From Commerce Spectrum Management Advisory Committee Working...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-28

    ... Spectrum Management Advisory Committee Working Group Process AGENCY: National Telecommunications and... ``lessons learned'' from NTIA's Commerce Spectrum Management Advisory Committee (CSMAC or Committee) Working... Constitution Avenue NW., Room 4830, Washington, DC 20230. Public input may be mailed to Commerce...

  20. Photoelectron Spectroscopy and Electronic Structure of Met-Car Ti_8C_12

    NASA Astrophysics Data System (ADS)

    Wang, Lai-Sheng; Li, San; Wu, Hongbin

    1997-03-01

    Photoelectron spectroscopy experiments are performed on metallocarbohedrene, Ti_8C_12^-, to obtain electronic structure information. The Ti_8C_12^- anion is produced by laser vaporization of a titanium carbide target. The spectra of Ti_8C_12^- consist of an intense threshold feature and two weaker features followed by a gap and more higher energy features. The electron affinity of Ti_8C_12 is found to be, 1.05 (=F10.05) (adiabatic) and 1.16 (=F10.05) eV (vertical). The surprisingly low electron affinity and the observed electronic features are interpreted using previously reported theoretical electronic structure information. We have also obtained preliminary results on other met-cars involving Zr, V, Nb and Cr. We found that the Zr met-car has a similar low EA as the Ti analog. However, all the other met-cars show much higher EAs and the EAs increase from Ti to V to Cr met-cars. *Pacific Northwest National Laboratory is operated for the U.S. Department of Energy by Battelle under contract DE-AC06-76RLO 1830.

  1. Stability, electronic structure, and vibrational modes of the Ti8C12 dimer

    NASA Astrophysics Data System (ADS)

    Baruah, Tunna; Pederson, Mark R.

    2002-12-01

    We present our density-functional results of the geometry, electronic structure, and dissociation energy of Ti8C12 dimer. We show that as opposed to the currently held view that Ti8C12 are highly stable monodispersed clusters, the neutral Ti8C12 clusters form covalent bonds and form stable dimers. We determine that the Ti atoms bond weakly (0.9 eV/bond) to organic ligands such as ammonia. Alternatively the metallocarbohedrene (Met-Car) dimer has a cohesive energy of 4.84 eV or approximately 1.2 eV per bond. While Met-Car dimers are stable, formation of these dimers may be quenched in an environment that contains a significant population of organic ligands. The ionization and dissociation energies of the dimer are of the same order, which prevents the observation of the dimer in ion mass spectroscopy. The analysis of the vibrational frequencies show the lowest-energy structure to be dynamically stable. We also present infrared-absorption and Raman-scattering spectra of the Ti8C12 dimer.

  2. Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation

    SciTech Connect

    Tsukahara, Tamotsu; Haniu, Hisao

    2011-03-25

    Research highlights: {yields} HTT2800 has a significant effect on intracellular lipid accumulation. {yields} HTT2800 reduced muscle-specific genes and led to the emergence of adipocyte-related genes. {yields} HT2800 converts the differentiation pathway of C2C12 myoblasts to that of adipoblast-like cells. -- Abstract: In this report, we sought to elucidate whether multiwall carbon nanotubes are involved in the modulation of the proliferation and differentiation of the skeletal muscle cell line C2C12. Skeletal muscle is a major mass peripheral tissue that accounts for 40% of total body weight and 50% of energy consumption. We focused on the differentiation pathway of myoblasts after exposure to a vapor-grown carbon fiber, HTT2800, which is one of the most highly purified carbon nanotubes. This treatment leads in parallel to the expression of a typical adipose differentiation program. We found that HTT2800 stimulated intracellular lipid accumulation in C2C12 cells. We have also shown by quantified PCR analysis that the expression of adipose-related genes was markedly upregulated during HTT2800 exposure. Taken together, these results suggest that HTT2800 specifically converts the differentiation pathway of C2C12 myoblasts to that of adipoblast-like cells.

  3. Identification of C12orf4 as a gene for autosomal recessive intellectual disability.

    PubMed

    Philips, A K; Pinelli, M; de Bie, C I; Mustonen, A; Määttä, T; Arts, H H; Wu, K; Roepman, R; Moilanen, J S; Raza, S; Varilo, T; Scala, G; Cocozza, S; Gilissen, C; van Gassen, K L I; Järvelä, I

    2017-01-01

    Intellectual disability (ID) is a major health problem in our society. Genetic causes of ID remain unknown because of its vast heterogeneity. Here we report two Finnish families and one Dutch family with affected individuals presenting with mild to moderate ID, neuropsychiatric symptoms and delayed speech development. By utilizing whole exome sequencing (WES), we identified a founder missense variant c.983T>C (p.Leu328Pro) in seven affected individuals from two Finnish consanguineous families and a deletion c.799_1034-429delinsTTATGA (p.Gln267fs) in one affected individual from a consanguineous Dutch family in the C12orf4 gene on chromosome 12. Both the variants co-segregated in the respective families as an autosomal recessive trait. Screening of the p.Leu328Pro variant showed enrichment in the North Eastern sub-isolate of Finland among anonymous local blood donors with a carrier frequency of 1:53, similar to other disease mutations with a founder effect in that region. To date, only one Arab family with a three affected individuals with a frameshift insertion variant in C12orf4 has been reported. In summary, we expand and establish the clinical and mutational spectrum of C12orf4 variants. Our findings implicate C12orf4 as a causative gene for autosomal recessive ID. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. 13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID31C12 ISIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. TIP TOP MINE. WOODEN STRUCTURE VISIBLE IN ID-31-C-12 ISIN UPPER LEFT. ALSO VISIBLE ARE TWO VERTICALLY BURIED LOGS ASSOCIATED WITH CABLES, ONE OF WHICH IS VISIBLE IN LOWER RIGHT. CAMERA POINTED SOUTH. - Florida Mountain Mining Sites, Tip Top Mine, West face Florida Mountain, approximately 150 feet below summit, Silver City, Owyhee County, ID

  5. C-12/C-13 Ratio in Ethane on Titan and Implications for Methane's Replenishment

    NASA Technical Reports Server (NTRS)

    Jennings, Donald E.; Romani, Paul N.; Bjoraker, Gordon L.; Sada, Pedro V.; Nixon, Conor A.; Lunsford, Allen W.; Boyle, Robert J.; Hesman, Brigette E.; McCabe, George H.

    2009-01-01

    The C-12/C-13 abundance ratio in ethane in the atmosphere of Titan has been measured at 822 cm(sup -1) from high spectral resolution ground-based observations. The value 89(8), coincides with the telluric standard and also agrees with the ratio seen in the outer planets. It is almost identical to the result for ethane on Titan found by the composite infrared spectrometer (CIRS) on Cassini. The C-12/C-13 ratio for ethane is higher than the ratio measured in atmospheric methane by Cassini/Huygens GCMS, 82.3(l), representing an enrichment of C-12 in the ethane that might be explained by a kinetic isotope effect of approximately 1.1 in the formation of methyl radicals. If methane is being continuously resupplied to balance photochemical destruction, then we expect the isotopic composition in the ethane product to equilibrate at close to the same C-12/C-13 ratio as that in the supply. The telluric value of the ratio in ethane then implies that the methane reservoir is primordial.

  6. 40 CFR 721.9285 - Reaction products of formalin (37%) with amine C12.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Reaction products of formalin (37... Specific Chemical Substances § 721.9285 Reaction products of formalin (37%) with amine C12. Link to an... to reporting. (1) The chemical substance identified generically as reaction products of formalin...

  7. The interstellar (C-12)N/(C-13)N ratio toward Zeta Persei

    NASA Technical Reports Server (NTRS)

    Kaiser, Mary E.; Wright, Edward L.; Hawkins, Isabel

    1991-01-01

    High-resolution, high signal-to-noise observations of interstellar CN toward Zeta Per are performed to determine the C-12/C-13 isotope ratio in this line of sight. Observations of (C-12)N/(C-13)N in several diffuse clouds are performed to assess whether CN suffers from isotope-selective effects. Values are obtained which are higher than the value toward Zeta Oph determined by Crane and Hegyi (1988) and lower than the results toward the more reddened stars HD 21483 obtained by Meyer et al. (1989) and Palazzi et al. (1990). Theory and observations indicate the existence of a spatial C-12/C-13 gradient which decreases toward the Galactic center at an approximate rate of 12 percent/kpc at the distance of the solar radius. It is argued that since a gradient of this character cannot explain the CN results, isotope-selective effects provide a more likely explanation for the large range in (C-12)N/(C-13)N ratios.

  8. L-carnitine protects C2C12 cells against mitochondrial superoxide overproduction and cell death

    PubMed Central

    Le Borgne, Françoise; Ravaut, Gaétan; Bernard, Arnaud; Demarquoy, Jean

    2017-01-01

    AIM To identify and characterize the protective effect that L-carnitine exerted against an oxidative stress in C2C12 cells. METHODS Myoblastic C2C12 cells were treated with menadione, a vitamin K analog that engenders oxidative stress, and the protective effect of L-carnitine (a nutrient involved in fatty acid metabolism and the control of the oxidative process), was assessed by monitoring various parameters related to the oxidative stress, autophagy and cell death. RESULTS Associated with its physiological function, a muscle cell metabolism is highly dependent on oxygen and may produce reactive oxygen species (ROS), especially under pathological conditions. High levels of ROS are known to induce injuries in cell structure as they interact at many levels in cell function. In C2C12 cells, a treatment with menadione induced a loss of transmembrane mitochondrial potential, an increase in mitochondrial production of ROS; it also induces autophagy and was able to provoke cell death. Pre-treatment of the cells with L-carnitine reduced ROS production, diminished autophagy and protected C2C12 cells against menadione-induced deleterious effects. CONCLUSION In conclusion, L-carnitine limits the oxidative stress in these cells and prevents cell death. PMID:28289521

  9. Expression and purification of TAT-fused carbonic anhydrase III and its effect on C2C12 cell apoptosis induced by hypoxia/reoxygenation

    PubMed Central

    Shang, Xiliang; Bao, Yuanyuan; Ren, Huimin; Huang, He; Li, Yunxia

    2012-01-01

    Introduction Carbonic anhydrase III (CAIII) is remarkably abundant in slow skeletal muscles. It has multiple biological activities which could dissipate or resist some fatigue-related substances. In this study, we purified trans-activating transcriptional activator (TAT) fused CAIII protein and investigated its effect on C2C12 cell apoptosis induced by hypoxia/reoxygenation. Material and methods The CAIII and TAT-CAIII genes were constructed, cloned into plasmid pET28a and expressed in Escherichia coli BL21 (DE3). The fusion proteins were purified with a nickel-nitrilotriacetic acid affinity chromatography column and then verified by Western blot and phosphatase activity staining subsequently. The C2C12 cells were treated respectively with serum-free medium containing 1 μM TAT-CAIII or 1 μM CAIII for 1 h and the intracellular distributions of fusion proteins were observed by indirect immunofluorescence. The effect of TAT-CAIII on C2C12 cell apoptosis induced by hypoxia/reoxygenation was detected by flow cytometry. Results The CAIII and TAT-CAIII fusion proteins were expressed and purified successfully. After being cultured for 1 h, green fluorescence was visible in TAT-CAIII group cells under the fluorescence microscope, while no fluorescence was found in the CAIII group. Compared with the oxygen-glucose deprivation group, the apoptosis rate of C2C12 cells induced by hypoxia/reoxygenation in the TAT-CAIII group decreased significantly (p < 0.001). Conclusions The purified TAT-CAIII could be transferred into cells efficiently and clearly decreased the apoptosis rate of C2C12 cells induced by hypoxia/reoxygenation, which indicated that it had antioxidative activity. This study lays an experimental basis for future research on the relationship between CAIII and muscle fatigue. PMID:23056085

  10. Organisational Commitment and Committee Turnover of Volunteers in Sport.

    ERIC Educational Resources Information Center

    Cuskelly, Graham

    1998-01-01

    A survey of 328 volunteers serving on sports committees in Australia found that organizational commitment was higher in organizations with open decision making, conflict resolution, and attention to group process. Positive, effective committee functioning led to lower turnover. (SK)

  11. 78 FR 16757 - First Meeting: RTCA Tactical Operations Committee (TOC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-18

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION Federal Aviation Administration First Meeting: RTCA Tactical Operations Committee (TOC) AGENCY: Federal... Discussion Committee Operations FAA Taskings Discussion FAA Task Groups Discussion Anticipated Issues for TOC...

  12. 77 FR 11142 - Merchant Marine Personnel Advisory Committee: Intercessional Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-24

    ... SECURITY Coast Guard Merchant Marine Personnel Advisory Committee: Intercessional Meeting AGENCY: Coast... Marine Personnel Advisory Committee (MERPAC) will conduct an intercessional meeting so that a working group may discuss Task Statement 76, entitled ``Review of Performance Measures (Assessment Criteria...

  13. 77 FR 1944 - Merchant Marine Personnel Advisory Committee: Intercessional Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-12

    ... SECURITY Coast Guard Merchant Marine Personnel Advisory Committee: Intercessional Meeting AGENCY: Coast... Marine Personnel Advisory Committee (MERPAC) will conduct an intercessional meeting to facilitate working group discussion of Task Statement 76, entitled ``Review of Performance Measures (Assessment Criteria...

  14. Apoptosis in differentiating C2C12 muscle cells selectively targets Bcl-2-deficient myotubes.

    PubMed

    Schöneich, Christian; Dremina, Elena; Galeva, Nadezhda; Sharov, Victor

    2014-01-01

    Muscle cell apoptosis accompanies normal muscle development and regeneration, as well as degenerative diseases and aging. C2C12 murine myoblast cells represent a common model to study muscle differentiation. Though it was already shown that myogenic differentiation of C2C12 cells is accompanied by enhanced apoptosis in a fraction of cells, either the cell population sensitive to apoptosis or regulatory mechanisms for the apoptotic response are unclear so far. In the current study we characterize apoptotic phenotypes of different types of C2C12 cells at all stages of differentiation, and report here that myotubes of differentiated C2C12 cells with low levels of anti-apoptotic Bcl-2 expression are particularly vulnerable to apoptosis even though they are displaying low levels of pro-apoptotic proteins Bax, Bak and Bad. In contrast, reserve cells exhibit higher levels of Bcl-2 and high resistance to apoptosis. The transfection of proliferating myoblasts with Bcl-2 prior to differentiation did not protect against spontaneous apoptosis accompanying differentiation of C2C12 cells but led to Bcl-2 overexpression in myotubes and to significant protection from apoptotic cell loss caused by exposure to hydrogen peroxide. Overall, our data advocate for a Bcl-2-dependent mechanism of apoptosis in differentiated muscle cells. However, downstream processes for spontaneous and hydrogen peroxide induced apoptosis are not completely similar. Apoptosis in differentiating myoblasts and myotubes is regulated not through interaction of Bcl-2 with pro-apoptotic Bcl-2 family proteins such as Bax, Bak, and Bad.

  15. Apoptosis in differentiating C2C12 muscle cells selectively targets Bcl-2-deficient myotubes

    PubMed Central

    Schoneich, Christian; Dremina, Elena; Galeva, Nadezhda; Sharov, Victor

    2014-01-01

    Muscle cell apoptosis accompanies normal muscle development and regeneration, as well as degenerative diseases and aging. C2C12 murine myoblast cells represent a common model to study muscle differentiation. Though it was already shown that myogenic differentiation of C2C12 cells is accompanied by enhanced apoptosis in a fraction of cells, either the cell population sensitive to apoptosis or regulatory mechanisms for the apoptotic response are unclear so far. In the current study we characterize apoptotic phenotypes of different types of C2C12 cells at all stages of differentiation, and report here that myotubes of differentiated C2C12 cells with low levels of anti-apoptotic Bcl-2 expression are particularly vulnerable to apoptosis even though they are displaying low levels of pro-apoptotic proteins Bax, Bak and Bad. In contrast, reserve cells exhibit higher levels of Bcl-2 and high resistance to apoptosis. The transfection of proliferating myoblasts with Bcl-2 prior to differentiation did not protect against spontaneous apoptosis accompanying differentiation of C2C12 cell but led to Bcl-2 overexpression in myotubes and to significant protection from apoptotic cell loss caused by exposure to hydrogen peroxide. Overall, our data advocate for a Bcl-2-dependent mechanism of apoptosis in differentiated muscle cells. However, downstream processes for spontaneous and hydrogen peroxide induced apoptosis are not completely similar. Apoptosis in differentiating myoblasts and myotubes is regulated not through interaction of Bcl-2 with pro-apoptotic Bcl-2 family proteins such as Bax, Bak, and Bad. PMID:24129924

  16. Folic acid is necessary for proliferation and differentiation of C2C12 myoblasts.

    PubMed

    Hwang, Seong Y; Kang, Yong J; Sung, Bokyung; Jang, Jung Y; Hwang, Na L; Oh, Hye J; Ahn, Yu R; Kim, Hong J; Shin, Jin H; Yoo, Mi-Ae; Kim, Cheol M; Chung, Hae Y; Kim, Nam D

    2017-05-04

    Folic acid, a water soluble B vitamin, plays an important role in cellular metabolic activities, such as functioning as a cofactor in one-carbon metabolism for DNA and RNA synthesis as well as nucleotide and amino acid biosynthesis in the body. A lack of dietary folic acid can lead to folic acid deficiency and result in several health problems, including macrocytic anemia, elevated plasma homocysteine, cardiovascular disease, birth defects, carcinogenesis, muscle weakness, and walking difficulty. However, the effect of folic acid deficiency on skeletal muscle development and its molecular mechanisms are unknown. We, therefore, investigated the effect of folic acid deficiency on myogenesis in skeletal muscle cells and found that folic acid deficiency induced proliferation inhibition and cell cycle breaking as well as cellular senescence in C2C12 myoblasts, implying that folic acid deficiency influences skeletal muscle development. Folic acid deficiency also inhibited differentiation of C2C12 myoblasts and induced deregulation of the cell cycle exit and many cell cycle regulatory genes. It inhibited expression of muscle-specific marker MyHC as well as myogenic regulatory factor (myogenin). Moreover, immunocytochemistry and Western blot analyses revealed that DNA damage was more increased in folic acid-deficient medium-treated differentiating C2C12 cells. Furthermore, we found that folic acid resupplementation reverses the effect on the cell cycle and senescence in folic acid-deficient C2C12 myoblasts but does not reverse the differentiation of C2C12 cells. Altogether, the study results suggest that folic acid is necessary for normal development of skeletal muscle cells. © 2017 Wiley Periodicals, Inc.

  17. Citizen Advisory Committees.

    ERIC Educational Resources Information Center

    Miller, Leann R.

    This guide, describing community involvement through citizen advisory committees, is a summary of the literature on such committees. Its main concern is district committees created by school boards. Citations in the bibliography contain all points of view on committees and present many alternatives on most of the topics covered in the guide.…

  18. Student Affairs Committee.

    ERIC Educational Resources Information Center

    Goodale, Thomas G.

    1984-01-01

    The agenda, structure, and responsibilities of a board of trustees standing committee--the campus student affairs committee--are discussed. The size, selection, and rotation of student affairs committee members will vary with the traditions and policies of the individual institution. If possible, the committee should include students among its…

  19. Expression of Glucose Transporter 4 (GLUT4) is Increased by Cinnamaldehyde in C2C12 Mouse Muscle Cells

    PubMed Central

    Nikzamir, Abdolrahim; Palangi, Alireza; Kheirollaha, Alireza; Tabar, Hashemi; Malakaskar, Alimohamad; Shahbazian, Hajieh; Fathi, Mohammad

    2014-01-01

    Background: In diabetes mellitus because of the absence or insufficient sensitivity to insulin, glucose transporter protein in cell membrane, glucose transporter 4, is decreased. GLUT4 is the major glucose transporter in skeletal muscle and adipose tissue, which is under control of insulin. It remains, however, unclear whether cinnamaldehyde plays a regulatory role(s) or not. Objectives: The objective of this study was to investigate the effects of cinnamaldehyde on GLUT4 gene expression. Materials and Methods: This study was an experimental trial. Tests were performed in triplicates. This study examined effects of cinnamaldehyde on Glut4 gene expression in C2C12 skeletal muscle cells by using Real Time PCR. C2C12 myoblasts were cultured in DMEM + 10 % FBS. After differentiation of myoblasts to myotubes, the cells were serum deprived for 5 hours and then treated with 10, 20, or 50 µM of cinnamaldehyde for 1 hour. Results: Our data revealed a significant increase in the expression of Glut4 in cinnamaldehyde treated cells. In addition, GLUT4 mRNA level was increased in a dose dependent manner. Analyses were performed using the SPSS 16 for Windows software. Differences between the groups were determined by one-way ANOVA. Conclusions: These results demonstrate that cinnamaldehyde up regulates the expression of mouse skeletal muscle GLUT4 gene expression. PMID:24719730

  20. Framework for development of physician competencies in genomic medicine: report of the Competencies Working Group of the Inter-Society Coordinating Committee for Physician Education in Genomics.

    PubMed

    Korf, Bruce R; Berry, Anna B; Limson, Melvin; Marian, Ali J; Murray, Michael F; O'Rourke, P Pearl; Passamani, Eugene R; Relling, Mary V; Tooker, John; Tsongalis, Gregory J; Rodriguez, Laura L

    2014-11-01

    Completion of the Human Genome Project, in conjunction with dramatic reductions in the cost of DNA sequencing and advances in translational research, is gradually ushering genomic discoveries and technologies into the practice of medicine. The rapid pace of these advances is opening up a gap between the knowledge available about the clinical relevance of genomic information and the ability of clinicians to include such information in their medical practices. This educational gap threatens to be rate limiting to the clinical adoption of genomics in medicine. Solutions will require not only a better understanding of the clinical implications of genetic discoveries but also training in genomics at all levels of professional development, including for individuals in formal training and others who long ago completed such training. The National Human Genome Research Institute has convened the Inter-Society Coordinating Committee for Physician Education in Genomics (ISCC) to develop and share best practices in the use of genomics in medicine. The ISCC has developed a framework for development of genomics practice competencies that may serve as a starting point for formulation of competencies for physicians in various medical disciplines.

  1. Probing the possibility of a C-12/C-13 galactic abundance gradient

    NASA Technical Reports Server (NTRS)

    Hawkins, Isabel

    1990-01-01

    High S/N (equal to or greater than 500) observations of interstellar CH+ with the 3.0 m telescope were performed at Lick Observatory and with the 4.0 m telescope at CTIO, of the reddened, early-type stars HD 183143, HD 24432, and HD 157038 in an effort to probe the existence of a C-12/C-13 abundance gradient in our Galaxy. Previous very high quality optical observations of interstellar CH+ toward five stars within 1 kpc of the Sun have yielded a precise weighted mean C-12/C-13 isotope ratio of 43 plus or minus 4 (1 sigma) (Hawkins, Jura, and Meyer 1985; Hawkins and Jura 1987). The isotope ratios derived toward four lines of sight in the local ISM are uniform within 12 percent. The similarity among these carbon isotope ratios determined in diffuse clouds possessing different physical conditions precludes the possibility that the CH+ molecule is suffering from isotope selective effects in these regions. The precise C-12/C-13 derivable from high quality observations of (12)CH+ and (13)CH+ provide the unique opportunity to probe the homogeneity of the ISM in a large scale and the history of nucleosynthesis in our Galaxy. Since CH+ seems to be the most sensitive probe of C-12/C-13 in the diffuse ISM, observations toward more distant stars located up to 2.5 kpc from the Sun are the best way to study the possibility of a Galactic (C-12/C-13) abundance gradient. The researcher obtained 4232 angstrom data toward all three of the stars mentioned above, 3957 angstrom data toward HD 183143 and HD 157038, and 3745 angstrom data toward HD 157038. Because of the poorer quality of the HD 24432 spectrum, and its weaker CH+ absorption lines, the satellite (13)CH+ line was not detected, and thus only a lower limit on the C-12/C-13 ratio toward this star was obtained. The results obtained from careful reduction and analysis of the data toward these stars in the Northern and Southern Hemispheres are presented.

  2. Bioconcentration and toxicity of dodecylbenzene sulfonate (C12LAS) to aquatic organisms exposed in experimental streams.

    PubMed

    Versteeg, D J; Rawlings, J M

    2003-02-01

    Fish, mollusks, and crustaceans were caged in the tail pool of streams during a C(12)LAS (dodecyl benzene sulfonate) model ecosystem experimental program. Bioconcentration of total C(12)LAS and individual isomers and acute and chronic toxicity were investigated during this study. Toxicity endpoints were based on water and tissue (i.e., body burden) concentrations at which adverse effects were observed. At 32 days, total C(12)LAS bioconcentration factors (BCFs) for the fathead minnow and three invertebrate species ranged from 9 to 116. In general, bioconcentration was affected by isomer position, exposure concentration, and species. BCF values tended to decrease as isomer position moved from external (e.g., 2-phenyl) to internal (e.g., 5,6-phenyl). BCFs also decreased as exposure concentration increased. Mean acute 4-d LC(50) values ranged from 1.5 to >3.0 mg/L for the six species tested. Lethal body burdens associated with 50% mortality (LBB(50)) varied from 0.21 to 0.60 mmole/kg (wet weight). During the 32-day chronic exposures, the EC(20) values were 0.27 (0.204-0.352), 0.95 (0.597-1.29), and approximately 1.0 mg/L for Corbicula (length), Hyalella (survival), and fathead minnow (survival), respectively. At these EC(20) values, C(12)LAS body burdens were 0.035, 0.23, and 0.19 mmoles/kg wet weight in Corbicula, Hyalella, and fathead minnow, respectively. Fish exposed to wastewater treatment plant effluent had total C(12)LAS tissue concentrations ranging from 0.0005 to 0.0039 mmoles/kg wet weight. These concentrations are approximately 45-360 times below the tissue concentration associated with subtle effects in the model ecosystem stream exposures. Total C(12)LAS body burdens in feral and caged Corbicula exposed to WWTP effluents were approximately 0.0013 mmoles/kg; approximately 25-fold below concentrations associated with effects in stream exposures.

  3. Cobalt triggers necrotic cell death and atrophy in skeletal C2C12 myotubes

    SciTech Connect

    Rovetta, Francesca; Stacchiotti, Alessandra; Faggi, Fiorella; Catalani, Simona; Apostoli, Pietro; Fanzani, Alessandro; Aleo, Maria Francesca

    2013-09-01

    Severe poisoning has recently been diagnosed in humans having hip implants composed of cobalt–chrome alloys due to the release of particulate wear debris on polyethylene and ceramic implants which stimulates macrophagic infiltration and destroys bone and soft tissue, leading to neurological, sensorial and muscular impairments. Consistent with this premise, in this study, we focused on the mechanisms underlying the toxicity of Co(II) ions on skeletal muscle using mouse skeletal C2C12 myotubes as an in vitro model. As detected using propidium iodide incorporation, increasing CoCl{sub 2} doses (from 5 to 200 μM) affected the viability of C2C12 myotubes, mainly by cell necrosis, which was attenuated by necrostatin-1, an inhibitor of the necroptotic branch of the death domain receptor signaling pathway. On the other hand, apoptosis was hardly detectable as supported by the lack of caspase-3 and -8 activation, the latter resulting in only faint activation after exposure to higher CoCl{sub 2} doses for prolonged time points. Furthermore, CoCl{sub 2} treatment resulted in atrophy of the C2C12 myotubes which was characterized by the increased expression of HSP25 and GRP94 stress proteins and other typical 'pro-atrophic molecular hallmarks, such as early activation of the NF-kB pathway and down-regulation of AKT phosphorylation, followed by the activation of the proteasome and autophagy systems. Overall, these results suggested that cobalt may impact skeletal muscle homeostasis as an inducer of cell necrosis and myofiber atrophy. - Highlights: • The effects of cobalt on muscle myofibers in vitro were investigated. • Cobalt treatment mainly causes cell necrosis in skeletal C2C12 myotubes. • Cobalt impacts the PI3K/AKT and NFkB pathways and induces cell stress markers. • Cobalt induces atrophy of C2C12 myotubes through the activation of proteasome and autophagy systems. • Co treatment triggers NF-kB and PI3K/AKT pathways in C2C12 myotubes.

  4. 78 FR 16629 - Osage Negotiated Rulemaking Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-18

    ... means of improving the trust management of the Osage Mineral Estate, the Osage Tribal Trust Account, and... of the public may present, either orally or through written comments, information for the Committee... Committee is not able to read written public comments submitted into the record. Individuals or groups...

  5. The Benefits of Participating in Collaborative Committees.

    ERIC Educational Resources Information Center

    Belfiore, Mary Ellen

    A study was conducted to show the effect that participation in workplace committees has on committee members. The subjects were 34 members of Workplace Education Center (WEC) projects of ABC Canada who work on establishing and maintaining workplace literacy initiatives; they were interviewed by phone or in group discussions. The study found that,…

  6. The Benefits of Participating in Collaborative Committees.

    ERIC Educational Resources Information Center

    Belfiore, Mary Ellen

    A study was conducted to show the effect that participation in workplace committees has on committee members. The subjects were 34 members of Workplace Education Center (WEC) projects of ABC Canada who work on establishing and maintaining workplace literacy initiatives; they were interviewed by phone or in group discussions. The study found that,…

  7. C-13/C-12 ratio in methane from the flooded Amazon forest

    NASA Technical Reports Server (NTRS)

    Tyler, Stanley C.; Blake, Donald R.; Rowland, F. Sherwood

    1987-01-01

    The C1-C4 hydrocarbon concentrations and the C-13/C-12 ratio in CH4 detected in air samples collected in the Amazon region (3.5 deg S latitude and 5.9 deg W longitude) are analyzed. It is observed that the CH4 concentrations of each sample are enhanced over the background concentration for remote locations, and the C-13/C-12 ratio in this biogenic methane is depleted in C-13 relative to atmospheric CH4. It is concluded, from the C2-C4 hydrocarbon data, that minor yields of C2-C4 hyrocarbons are released coincident with CH4; however, the minor yields do not contribute to the regionally enhanced concentrations of hydrocarbons found in Amazonia and the global atmosphere release of C2-C4 compounds.

  8. Computational prediction of a simple cubic carbon allotrope consisting of C12 clusters

    NASA Astrophysics Data System (ADS)

    Bu, Kun; Li, Zhen-Zhen; Wang, Jian-Tao

    2017-08-01

    We identify by ab initio calculations a new simple cubic carbon phase in P a 3 ¯ symmetry, which has a 48-atom unit cell in all-sp3 bonding networks, thus termed SC48 carbon. It can be viewed as a crystalline form of C12 clusters or a combined structure of SC24 and BC12 carbon, but it is energetically more stable than the recently reported cubic carbon phases such as BC8, SC24, BC12, and fcc-C12. The structural stability is verified by phonon mode analysis. Electronic band and density of state calculations reveal that SC48 carbon is an insulator with a large direct band gap of 4.40 eV. Moreover, simulated x-ray diffraction patterns provide an excellent match to the distinct diffraction peaks found in milled fullerene soot. These results provide a solid foundation for further exploration of this new carbon allotrope.

  9. Rab8A regulates insulin-stimulated GLUT4 translocation in C2C12 myoblasts.

    PubMed

    Li, Hanbing; Ou, Liting; Fan, Jiannan; Xiao, Mei; Kuang, Cuifang; Liu, Xu; Sun, Yonghong; Xu, Yingke

    2017-02-01

    Rab proteins are important regulators of GLUT4 trafficking in muscle and adipose cells. It is still unclear which Rabs are involved in insulin-stimulated GLUT4 translocation in C2C12 myoblasts. In this study, we detect the colocalization of Rab8A with GLUT4 and the presence of Rab8A at vesicle exocytic sites by TIRFM imaging. Overexpression of dominant-negative Rab8A (T22N) diminishes insulin-stimulated GLUT4 translocation, while constitutively active Rab8A (Q67L) augments it. In addition, knockdown of Rab8A inhibits insulin-stimulated GLUT4 translocation, which is rescued by replenishment of RNAi-resistant Rab8A. Together, these results indicate an indispensable role for Rab8A in insulin-regulated GLUT4 trafficking in C2C12 cells.

  10. Low-level laser irradiation modulates cell viability and creatine kinase activity in C2C12 muscle cells during the differentiation process.

    PubMed

    Mesquita-Ferrari, Raquel Agnelli; Alves, Agnelo Neves; de Oliveira Cardoso, Vinicius; Artilheiro, Paola Pelegrineli; Bussadori, Sandra Kalil; Rocha, Lilia Alves; Nunes, Fábio Daumas; Fernandes, Kristianne Porta Santos

    2015-11-01

    Low-level laser irradiation (LLLI) is increasingly used to treat musculoskeletal disorders, with satisfactory results described in the literature. Skeletal muscle satellite cells play a key role in muscle regeneration. The aim of the present study was to evaluate the effect of LLLI on cell viability, creatine kinase (CK) activity, and the expression of myogenic regulatory factors in C2C12 myoblasts during the differentiation process. C2C12 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 2% horse serum and submitted to irradiation with GaAlAs diode laser (wavelength, 780 nm; output power, 10 mW; energy density, 5 J/cm2). Cell viability and the expression of myogenic regulatory factors were assessed 24, 48, and 72 h after irradiation by 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay and quantitative real-time polymerase chain reaction (RT-qPCR), respectively. CK activity was analyzed at 24 and 72 h. An increase in cell viability was found in the laser group in comparison to the control group at all evaluation times. CK activity was significantly increased in the laser group at 72 h. Myogenin messenger RNA (mRNA) demonstrated a tendency toward an increase in the laser group, but the difference in comparison to the control group was non-significant. In conclusion, LLLI was able to modulate cell viability and CK activity in C2C12 myoblasts during the differentiation process.

  11. Effects of margarine supplemented with t10c12 and C9T11 CLA on atherosclerosis and steatosis in apoE/LDLR -/- mice.

    PubMed

    Kostogrys, R B; Franczyk-Żarów, M; Maślak, E; Gajda, M; Mateuszuk, Ł; Chłopicki, S

    2012-05-01

    The objective of this study was to evaluate functional effects of margarine supplemented with individual CLA isomers trans-10, cis-12 and cis-9, trans-11 in apoE/LDLR -/- mice. In LONG experiment (LONG), two-month old mice with no atherosclerosis were assigned to experimental groups and fed for the next 4 months. In SHORT experiment (SHORT), four-month old mice, with pre-established atherosclerosis, were assigned to experimental groups and fed for the next 2 months. The experimental diets were: AIN-93G (margarine), AIN-93G + 0.5% trans-10, cis-12 CLA (t10c12), and AIN-93G + 0.5% cis-9, trans-11 CLA (c9t11). In both experiments (LONG and SHORT), liver weight was significantly (P<0.05) increased in mice fed t10c12 CLA. Hepatic steatosis was found in animals fed t10c12 diet and no signs of the steatosis was observed in mice fed c9t11 CLA. Dietary treatments with t10c12 CLA significantly increased total plasma cholesterol and plasma triacylglycerols. There were no isomer-specific effects of CLA isomers on area of atherosclerotic plaque in aortic root. In conclusion, t10c12 CLA significantly increased liver weight in mice in LONG and SHORT experiments. Our results do not support the notion that CLA isomer supplementation to the margarine possess anti-atheroclerotic effect. Therefore, no isomer-specific effects of CLA on development of atherosclerosis were observed.

  12. Lysine suppresses protein degradation through autophagic-lysosomal system in C2C12 myotubes.

    PubMed

    Sato, Tomonori; Ito, Yoshiaki; Nedachi, Taku; Nagasawa, Takashi

    2014-06-01

    Muscle mass is determined between protein synthesis and protein degradation. Reduction of muscle mass leads to bedridden condition and attenuation of resistance to diseases. Moreover, bedridden condition leads to additional muscle loss due to disuse muscle atrophy. In our previous study (Sato et al. 2013), we showed that administered lysine (Lys), one of essential amino acid, suppressed protein degradation in skeletal muscle. In this study, we investigated that the mechanism of the suppressive effects of Lys on skeletal muscle proteolysis in C2C12 cell line. C2C12 myotubes were incubated in the serum-free medium containing 10 mM Lys or 20 mM Lys, and myofibrillar protein degradation was determined by the rates of 3-methylhistidine (MeHis) release from the cells. The mammalian target of rapamycin (mTOR) activity from the phosphorylation levels of p70-ribosormal protein S6 kinase 1 and eIF4E-binding protein 1 and the autophagic-lysosomal system activity from the ratio of LC3-II/I in C2C12 myotubes stimulated by 10 mM Lys for 0-3 h were measured. The rates of MeHis release were markedly reduced by addition of Lys. The autophagic-lysosomal system activity was inhibited upon 30 min of Lys supplementation. The activity of mTOR was significantly increased upon 30 min of Lys supplementation. The suppressive effect of Lys on the proteolysis by the autophagic-lysosomal system was maintained partially when mTOR activity was inhibited by 100 nM rapamycin, suggesting that some regulator other than mTOR signaling, for example, Akt, might also suppress the autophagic-lysosomal system. From these results, we suggested that Lys suppressed the activity of the autophagic-lysosomal system in part through activation of mTOR and reduced myofibrillar protein degradation in C2C12 myotubes.

  13. Tetramethylpyrazine protects palmitate-induced oxidative damage and mitochondrial dysfunction in C2C12 myotubes.

    PubMed

    Gao, Xin; Zhao, Xiao-long; Zhu, Yan-hui; Li, Xiao-mu; Xu, Qiong; Lin, Huan-dong; Wang, Ming-wei

    2011-04-25

    Tetramethylpyrazine (TMP), one of the active ingredients isolated from a Chinese herbal prescription, possesses protective effects against oxidative stress caused by high glucose in endothelial cells. In this study, the role of TMP in preventing muscle cells from palmitate-induced oxidative damage was investigated and the possible mechanisms of action elucidated. Mitochondrial reactive oxygen species (ROS) were measured in C2C12 myotubes, a palmitate-induced oxidative stress cell model, with or without TMP. Both mitochondrial membrane potential (MMP) and oxygen consumption were assessed in conjunction with quantification of mitochondrial DNA and mitochondrial biogenesis-related factors, such as peroxisome proliferator-activated receptor-γ coactivator 1 α (PGC1α), nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (Tfam), by real-time polymerase chain reaction. Expression of mitochondrial respiratory chain complex III as an index of mitochondrial function was evaluated by immunoblotting, and glucose transport into the C2C12 myotube examined by analyzing 2-deoxy-[(3)H]glucose uptake. TMP significantly alleviated palmitate-induced mitochondrial ROS production, mitigated mitochondrial dysfunction and increased D-loop mRNA expression as compared with the control. This was accompanied by a marked reversal of palmitate-induced down-regulation in the expression of mitochondrial biogenesis-related factors (PGC1α, NRF1 and Tfam) and decreased glucose uptake in C2C12 myotubes. As a result, cell respiration, as reflected by the elevated expression of mitochondrial respiratory chain complex III and oxygen consumption, was enhanced. TMP is capable of protecting C2C12 myotubes against palmitate-induced oxidative damage and mitochondrial dysfunction, and improving glucose uptake in muscle cells partially through the up-regulation of mitochondrial biogenesis. Copyright © 2011 Elsevier Inc. All rights reserved.

  14. GDF11 does not improve the palmitate induced insulin resistance in C2C12.

    PubMed

    Jing, Y-Y; Li, D; Wu, F; Gong, L-L; Li, R

    2017-04-01

    GDF11 (Growth Differentiation factor 11) has been reported to rejuvenate skeletal muscle, heart and brain in aged mice, and the aged skeletal muscle is closely related to insulin resistance. We wondered whether GDF11 has an effect on skeletal muscle insulin resistance. High fat diet induced obese mice with insulin resistance were established in vivo. Palmitate-induced insulin resistance in C2C12 myotubes was established in vitro. The mRNA expression of GDF11, GLUT4, IRS-1 (insulin receptor substrate-1) and PGC-1α (peroxisome proliferator-activated receptor-gamma coactivator 1) were tested by reverse transcriptase-polymerase chain reaction (RT-PCR). The protein level of GDF11 and PGC-1α were detected by Western blot. The glucose uptake was measured by 2NBDG uptake assay. In high fat diet induced obese mice, both serum level of GDF11 and the expression of GDF11 in skeletal muscle decreased. Similarly, the expression of GDF11 also reduced in palmitate-treated C2C12 myotubes. In vitro, the glucose uptake and the expression of GLUT4, IRS-1 and PGC-1α significantly decreased after palmitate intervention, but GDF11 treatment did not reverse the reduction of glucose uptake and the expression of GLUT4, IRS-1 and PGC-1α in C2C12 myotubes. We firstly confirmed that the expression of GDF11 decreased both in the skeletal muscle of obese mice and palmitate-treated myotubes, but supplementation GDF11 does not ameliorate the palmitate-induced insulin resistance in C2C12 myotubes.

  15. IGF-1 prevents simvastatin-induced myotoxicity in C2C12 myotubes.

    PubMed

    Bonifacio, Annalisa; Sanvee, Gerda M; Brecht, Karin; Kratschmar, Denise V; Odermatt, Alex; Bouitbir, Jamal; Krähenbühl, Stephan

    2017-05-01

    Statins are generally well tolerated, but treatment with these drugs may be associated with myopathy. The mechanisms of statin-associated myopathy are not completely understood. Statins inhibit AKT phosphorylation by an unclear mechanism, whereas insulin-like growth factor (IGF-1) activates the IGF-1/AKT signaling pathway and promotes muscle growth. The aims of the study were to investigate mechanisms of impaired AKT phosphorylation by simvastatin and to assess effects of IGF-1 on simvastatin-induced myotoxicity in C2C12 myotubes. C2C12 mouse myotubes were exposed to 10 μM simvastatin and/or 10 ng/mL IGF-1 for 18 h. Simvastatin inhibited the IGF-1/AKT signaling pathway, resulting in increased breakdown of myofibrillar proteins, impaired protein synthesis and increased apoptosis. Simvastatin inhibited AKT S473 phosphorylation, indicating reduced activity of mTORC2. In addition, simvastatin impaired stimulation of AKT T308 phosphorylation by IGF-1, indicating reduced activation of the IGF-1R/PI3K pathway by IGF-1. Nevertheless, simvastatin-induced myotoxicity could be at least partially prevented by IGF-1. The protective effects of IGF-1 were mediated by activation of the IGF-1R/AKT signaling cascade. Treatment with IGF-1 also suppressed muscle atrophy markers, restored protein synthesis and inhibited apoptosis. These results were confirmed by normalization of myotube morphology and protein content of C2C12 cells exposed to simvastatin and treated with IGF-1. In conclusion, impaired activity of AKT can be explained by reduced function of mTORC2 and of the IGF-1R/PI3K pathway. IGF-1 can prevent simvastatin-associated cytotoxicity and metabolic effects on C2C12 cells. The study gives insight into mechanisms of simvastatin-associated myotoxicity and provides potential targets for therapeutic intervention.

  16. Leucine Modulates Mitochondrial Biogenesis and SIRT1-AMPK Signaling in C2C12 Myotubes

    PubMed Central

    Liang, Chunzi; Curry, Benjamin J.; Brown, Patricia L.; Zemel, Michael B.

    2014-01-01

    Previous studies from this laboratory demonstrate that dietary leucine protects against high fat diet-induced mitochondrial impairments and stimulates mitochondrial biogenesis and energy partitioning from adipocytes to muscle cells through SIRT1-mediated mechanisms. Moreover, β-hydroxy-β-methyl butyrate (HMB), a metabolite of leucine, has been reported to activate AMPK synergistically with resveratrol in C2C12 myotubes. Therefore, we hypothesize that leucine-induced activation of SIRT1 and AMPK is the central event that links the upregulated mitochondrial biogenesis and fatty acid oxidation in skeletal muscle. Thus, C2C12 myotubes were treated with leucine (0.5 mM), alanine (0.5 mM), valine (0.5 mM), EX527 (SIRT1 inhibitor, 25 μM), and Compound C (AMPK inhibitor, 25 μM) alone or in combination to determine the roles of AMPK and SIRT1 in leucine-modulation of energy metabolism. Leucine significantly increased mitochondrial content, mitochondrial biogenesis-related genes expression, fatty acid oxidation, SIRT1 activity and gene expression, and AMPK phosphorylation in C2C12 myotubes compared to the controls, while EX527 and Compound C markedly attenuated these effects. Furthermore, leucine treatment for 24 hours resulted in time-dependent increases in cellular NAD+, SIRT1 activity, and p-AMPK level, with SIRT1 activation preceding that of AMPK, indicating that leucine activation of SIRT1, rather than AMPK, is the primary event. PMID:25400942

  17. Leptin rapidly activates PPARs in C2C12 muscle cells

    SciTech Connect

    Bendinelli, Paola; Piccoletti, Roberta . E-mail: Roberta.Piccoletti@unimi.it; Maroni, Paola

    2005-07-08

    Experimental evidence suggests that leptin operates on the tissues, including skeletal muscle, also by modulating gene expression. Using electrophoretic mobility shift assays, we have shown that physiological doses of leptin promptly increase the binding of C2C12 cell nuclear extracts to peroxisome proliferator-activated receptor (PPAR) response elements in oligonucleotide probes and that all three PPAR isoforms participate in DNA-binding complexes. We pre-treated C2C12 cells with AACOCF{sub 3}, a specific inhibitor of cytosolic phospholipase A{sub 2} (cPLA{sub 2}), an enzyme that supplies ligands to PPARs, and found that it abrogates leptin-induced PPAR DNA-binding activity. Leptin treatment significantly increased cPLA{sub 2} activity, evaluated as the release of [{sup 3}H]arachidonic acid from pre-labelled C2C12 cells, as well as phosphorylation. Further, using MEK1 inhibitor PD-98059 we showed that leptin activates cPLA{sub 2} through ERK induction. These results support a direct effect of leptin on skeletal muscle cells, and suggest that the hormone may modulate muscle transcription also by precocious activation of PPARs through ERK-cPLA{sub 2} pathway.

  18. Arecoline inhibits myogenic differentiation of C2C12 myoblasts by reducing STAT3 phosphorylation.

    PubMed

    Chang, Yung-Fu; Liu, Ting-Yuan; Liu, Shao-Tung; Tseng, Chao-Neng

    2012-10-01

    Areca nut (Areca catechu) is chewed regularly as a medical and psychoactive food by about 10% of the world population, in countries including India, Taiwan and parts of Southern Asia. Areca nut chewing during pregnancy has been associated with both lower birth weight and premature birth. Animals of low birth weights showed retardation of muscle development. Our previous study showed that arecoline, the major areca alkaloid, decreased the number of implanted embryos. Here we sought to determine the effects of arecoline in myogenic differentiation by in vitro assays using C2C12 myoblast cells. The results showed that arecoline higher than 0.4mM significantly increased apoptosis and decreased viability of C2C12 cells. Morphometric measurements of myotube formation and analyses of myogenic markers, myosin heavy chain and myogenin, revealed that myogenic differentiation was inhibited by 0.04-0.08 mM arecoline. Moreover, phosphorylated but not total STAT3 was significantly inhibited by arecoline during myotube formation. These results indicate that arecoline inhibits the myogenic differentiation of C2C12 cells by reducing the activation of STAT3, an upstream regulator of myogenesis. Improved understanding of the effects of arecoline during myogenic differentiation may help to establish public health policies and to develop potential treatments for such patients. Copyright © 2012 Elsevier Ltd. All rights reserved.

  19. IL-12 involvement in myogenic differentiation of C2C12 in vitro.

    PubMed

    Romanazzo, Sara; Forte, Giancarlo; Morishima, Keisuke; Taniguchi, Akiyoshi

    2015-03-01

    Recently, the extracellular microenvironment has been shown to be critical for the correct differentiation of stem cells to specific tissues. Many factors, including physical (e.g. biomaterial stiffness and topography) and biological (as growth factors, cytokines and chemokines) components, cooperate to create an ideal microenvironment for muscle stem cells, with many of these factors having been widely investigated. We previously demonstrated that the use of non-proliferating muscle-specific and unrelated cells as feeder layers for skeletal muscle progenitor cell differentiation resulted in significant differences in the ability to form myotubes, suggesting the importance of biological factors in myogenic differentiation. In this study, we investigated the biological factors involved in this process, analyzing the expression profile of 84 genes coding for cytokines and chemokines. We successfully identified a novel role for the cytokine IL-12 in the myogenic differentiation of C2C12 mouse skeletal muscle cells. Experiments involving the overexpression or silencing of the IL-12 gene in C2C12 showed that IL-12 enhanced the myogenic differentiation process. Moreover, when IL-12 was overexpressed in non-biologically related feeder cells, the new co-culture system was able to improve myogenic differentiation of C2C12 seeded on top. Although IL-12 is known to be a cytokine involved in inflammatory responses, it also appears to be involved in the myogenic differentiation process, acting as a positive regulator of this mechanism. This fact is expected to prove to be important for the development of functional biomaterials.

  20. First Direct Measurement of (12)C((12)C,n)(23)Mg at Stellar Energies.

    PubMed

    Bucher, B; Tang, X D; Fang, X; Heger, A; Almaraz-Calderon, S; Alongi, A; Ayangeakaa, A D; Beard, M; Best, A; Browne, J; Cahillane, C; Couder, M; deBoer, R J; Kontos, A; Lamm, L; Li, Y J; Long, A; Lu, W; Lyons, S; Notani, M; Patel, D; Paul, N; Pignatari, M; Roberts, A; Robertson, D; Smith, K; Stech, E; Talwar, R; Tan, W P; Wiescher, M; Woosley, S E

    2015-06-26

    Neutrons produced by the carbon fusion reaction (12)C((12)C,n)(23)Mg play an important role in stellar nucleosynthesis. However, past studies have shown large discrepancies between experimental data and theory, leading to an uncertain cross section extrapolation at astrophysical energies. We present the first direct measurement that extends deep into the astrophysical energy range along with a new and improved extrapolation technique based on experimental data from the mirror reaction (12)C((12)C,p)(23)Na. The new reaction rate has been determined with a well-defined uncertainty that exceeds the precision required by astrophysics models. Using our constrained rate, we find that (12)C((12)C,n)(23)Mg is crucial to the production of Na and Al in pop-III pair instability supernovae. It also plays a nonnegligible role in the production of weak s-process elements, as well as in the production of the important galactic γ-ray emitter (60)Fe.

  1. Myostatin inhibits cell proliferation and protein synthesis in C2C12 muscle cells.

    PubMed

    Taylor, W E; Bhasin, S; Artaza, J; Byhower, F; Azam, M; Willard, D H; Kull, F C; Gonzalez-Cadavid, N

    2001-02-01

    Myostatin mutations in mice and cattle are associated with increased muscularity, suggesting that myostatin is a negative regulator of skeletal muscle mass. To test the hypothesis that myostatin inhibits muscle cell growth, we examined the effects of recombinant myostatin in mouse skeletal muscle C2C12 cells. After verification of the expression of cDNA constructs in a cell-free system and in transfected Chinese hamster ovary cells, the human recombinant protein was expressed as the full-length (375-amino acid) myostatin in Drosophila cells (Mst375D), or the 110-amino acid carboxy-terminal protein in Escherichia coli (Mst110EC). These proteins were identified by immunoblotting and were purified. Both Mst375D and Mst110EC dose dependently inhibited cell proliferation (cell count and Formazan assay), DNA synthesis ([3H]thymidine incorporation), and protein synthesis ([1-14C]leucine incorporation) in C2C12 cells. The inhibitory effects of both proteins were greater in myotubes than in myoblasts. Neither protein had any significant effects on protein degradation or apoptosis. In conclusion, recombinant myostatin proteins inhibit cell proliferation, DNA synthesis, and protein synthesis in C2C12 muscle cells, suggesting that myostatin may control muscle mass by inhibiting muscle growth or regeneration.

  2. 41 CFR 102-3.40 - What types of committees or groups are not covered by the Act and this part?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... individual advice. Any group that meets with a Federal official(s), including a public meeting, where advice... assembled to exchange facts or information. Any group that meets with a Federal official(s) for the purpose... 204(b) of the Unfunded Mandates Reform Act of 1995, 2 U.S.C. 1534(b), OMB Memorandum M-95-20, dated...

  3. 41 CFR 102-3.40 - What types of committees or groups are not covered by the Act and this part?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... individual advice. Any group that meets with a Federal official(s), including a public meeting, where advice... assembled to exchange facts or information. Any group that meets with a Federal official(s) for the purpose... 204(b) of the Unfunded Mandates Reform Act of 1995, 2 U.S.C. 1534(b), OMB Memorandum M-95-20, dated...

  4. Targeting prostate carcinoma by G3-C12 peptide conjugated N-(2-hydroxypropyl)methacrylamide copolymers.

    PubMed

    Yang, Yang; Li, Lian; Zhou, Zhou; Yang, Qingqing; Liu, Chong; Huang, Yuan

    2014-10-06

    Prostate carcinoma is the second leading cause of cancer-related deaths. Increased expression of membrane-bound galectin-3 by prostate carcinoma cell has been found to correlate with more poorly differentiated and increased metastatic potential. In the present study, different amount of galectin-3-binding peptide, G3-C12 (the sequence ANTPCGPYTHDCPVKR), was attached to N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers as targeting moiety. The results of qPCR and competitive binding test indicated that the expression level of galectin-3 in two metastatic prostate carcinoma cell lines (PC-3 and DU145 cells) could be significantly suppressed by the addition of G3-C12-modified HPMA copolymers (PG1 and PG2), demonstrating the high affinity of PG1 and PG2 to galectin-3. Due to the multivalent effects of moieties, the uptake of copolymers was remarkably enhanced with the increasing amount of conjugated G3-C12 peptide. A higher internalization of PG1 and PG2 occurred in PC-3 cells via caveolin- and clathrin-mediated endocytosis, whereas a clathrin-mediated uptake process was involved in DU145 cells. The in vivo biodistribution and pharmacokinetics of nonmodified ((131)I-pHPMA) and G3-C12-modified ((131)I-PG1 and (131)I-PG2) copolymers were estimated on a well-established mice model bearing PC-3 xenografts by (131)I-SPECT-imaging. Higher tumor accumulation of (131)I-PG1 (1.60 ± 0.08% ID/g, p < 0.05) and (131)I-PG2 (1.54 ± 0.06% ID/g, p < 0.05) was observed compared with (131)I-pHPMA (1.19 ± 0.04% ID/g) at 2 h post-intravenous injection. Although the amount of conjugated G3-C12 peptide performed a remarkable in vitro effect on the affinity and internalization of HPMA copolymers to the galectin-3 overexpressed prostate carcinoma cells, the molecular weight and ligand modification all play important roles on their in vivo tumor accumulation.

  5. Automotive Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in automotive technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are used…

  6. Autobody Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in autobody technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings and are used as…

  7. Federal Advisory Committees: An Overview

    DTIC Science & Technology

    2009-04-16

    initiating a tradition of presidential use of outside expertise when, in 1794 , he appointed an ad hoc group of commissioners to investigate the Whiskey ... Rebellion .7 Since 1842, Congress has legislated control over federal advisory bodies — mostly by limiting funding and committee member pay. In 1842...reports aggregated advisory body information to Congress annually.12 7 " Whiskey Insurrection in

  8. Automotive Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in automotive technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are used…

  9. Electronic Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in electronic technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are used…

  10. Autobody Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in autobody technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings and are used as…

  11. Electronic Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in electronic technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are used…

  12. Monitoring the safety of a smallpox vaccination program in the United States: report of the joint Smallpox Vaccine Safety Working Group of the advisory committee on immunization practices and the Armed Forces Epidemiological Board.

    PubMed

    Neff, John; Modlin, John; Birkhead, Guthrie S; Poland, Gregory; Robertson, Rose Marie; Sepkowitz, Kent; Yancy, Clyde; Gardner, Pierce; Gray, Gregory C; Maurer, Toby; Siegel, Jane; Guerra, Fernando A; Berger, Tim; Flanders, W Dana; Shope, Robert

    2008-03-15

    In December 2002, the Centers for Disease Control and Prevention's Advisory Committee on Immunization Practices and the Department of Defense Armed Forces Epidemiological Board formed a joint Smallpox Vaccine Safety Working Group (SVS WG) to provide independent safety oversight for smallpox vaccination safety-monitoring systems. From January 2003 through June 2004, the SVS WG reviewed individual and aggregate safety data on postvaccination adverse events. Serious adverse events were rare because of careful education, prevaccination screening, and strict attention to vaccination-site management. Recent vaccinees safely cared for high-risk patients, adhering to recommended site care. Human immunodeficiency virus-infected individuals without severe immunosuppression had uncomplicated vaccination reactions. Epidemiological studies supported a causal relationship between myocarditis and/or pericarditis and smallpox vaccination. Data supported neutrality regarding hypothesized causal associations between vaccination and dilated cardiomyopathy or ischemic cardiac disease. The SVS WG concurs with recommendations to defer from vaccination any person with >/=3 ischemic cardiac disease risk factors.

  13. Congenital and acquired neutropenias consensus guidelines on therapy and follow-up in childhood from the Neutropenia Committee of the Marrow Failure Syndrome Group of the AIEOP (Associazione Italiana Emato-Oncologia Pediatrica).

    PubMed

    Fioredda, Francesca; Calvillo, Michaela; Bonanomi, Sonia; Coliva, Tiziana; Tucci, Fabio; Farruggia, Piero; Pillon, Marta; Martire, Baldassarre; Ghilardi, Roberta; Ramenghi, Ugo; Renga, Daniela; Menna, Giuseppe; Pusiol, Anna; Barone, Angelica; Gambineri, Eleonora; Palazzi, Giovanni; Casazza, Gabriella; Lanciotti, Marina; Dufour, Carlo

    2012-02-01

    The management of congenital and acquired neutropenias presents some differences according to the type of the disease. Treatment with recombinant human granulocyte-colony stimulating factor (G-CSF) is not standardized and scanty data are available on the best schedule to apply. The frequency and the type of longitudinal controls in patients affected with neutropenias are not usually discussed in the literature. The Neutropenia Committee of the Marrow Failure Syndrome Group (MFSG) of the Associazione Italiana di Emato-Oncologia Pediatrica (AIEOP) elaborated this document following design and methodology formerly approved by the AIEOP board. The panel of experts reviewed the literature on the topic and participated in a conference producing a document that includes recommendations on neutropenia treatment and timing of follow-up.

  14. 78 FR 45592 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an..., 2013. Marc Zlomek, Executive Secretary, Shipping Coordinating Committee, Department of State. BILLING...

  15. 77 FR 16316 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... . Dated: March 9, 2012. Brian Robinson, Executive Secretary, Shipping Coordinating Committee, Department...

  16. 76 FR 70529 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-14

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will...: November 7, 2011. Brian Robinson, Executive Secretary, Shipping Coordinating Committee, Department of State...

  17. 77 FR 12641 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-01

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an..., 2012. Brian Robinson, Executive Secretary, Shipping Coordinating Committee, Department of State...

  18. 77 FR 47490 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct open.... Brian Robinson, Executive Secretary, Shipping Coordinating Committee, Department of State. BILLING CODE...

  19. Taking Charge: Kindergartners' Planning and Leadership Talk During Committee Work.

    ERIC Educational Resources Information Center

    Lazarus, Peggy G.

    This study delineates features of the planning and leadership talk of kindergartners involved in committee work done in small groups. Subjects were 66 kindergarten children in 3 classes of one teacher. Committees were composed of four or five students and met during a 10-month period across 2 school years. the tasks of the committees were to: (1)…

  20. The Facilities Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Kaiser, Harvey H.

    2012-01-01

    This publication is part of an Association of Governing Boards of Universities and Colleges (AGB) series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices…

  1. The Investment Committee. Effective Committees. Board Basics.

    ERIC Educational Resources Information Center

    Biggs, John H.

    1997-01-01

    The investment committee of the college or university governing board is charged with determining, overseeing, and assessing the policies and processes by which institutional funds are invested. The committee has fiduciary duty to ensure that the terms of investment of donors' gifts are met and to maximize investment returns within an appropriate…

  2. The Audit Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Staisloff, Richard L.

    2011-01-01

    This publication is part of an Association of Governing Boards of Universities and Colleges (AGB) series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices…

  3. The Compensation Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Hyatt, Thomas K.

    2013-01-01

    This publication is part of an Association of Governing Boards of Universities and Colleges (AGB) series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices…

  4. The Executive Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Legon, Richard D.

    2012-01-01

    This publication is part of an Association of Governing Boards of Universities and Colleges (AGB) series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices…

  5. The Audit Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Staisloff, Richard L.

    2011-01-01

    This publication is part of an Association of Governing Boards of Universities and Colleges (AGB) series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices…

  6. The Investment Committee. Effective Committees. Board Basics.

    ERIC Educational Resources Information Center

    Biggs, John H.

    1997-01-01

    The investment committee of the college or university governing board is charged with determining, overseeing, and assessing the policies and processes by which institutional funds are invested. The committee has fiduciary duty to ensure that the terms of investment of donors' gifts are met and to maximize investment returns within an appropriate…

  7. The Executive Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Legon, Richard D.

    2012-01-01

    This publication is part of an Association of Governing Boards of Universities and Colleges (AGB) series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices…

  8. The Compensation Committee. AGB Effective Committee Series

    ERIC Educational Resources Information Center

    Hyatt, Thomas K.

    2013-01-01

    This publication is part of an Association of Governing Boards of Universities and Colleges (AGB) series devoted to strengthening the role of key standing committees of governing boards. While there is no optimum committee system for institutions of higher education, certain principles, practices, and procedures prevail. The best practices…

  9. Advisory Committee Handbook.

    ERIC Educational Resources Information Center

    Black Hawk Coll., Moline, IL.

    An advisory committee is generally comprised of persons outside the education profession who have specialized knowledge in a given area. The committee advises, makes recommendations, and gives service to the college and its students, instructors, and administrators. At Black Hawk College, there are four types of advisory committees: community,…

  10. 17β-estradiol upregulates oxytocin and the oxytocin receptor in C2C12 myotubes

    PubMed Central

    Berio, Enrica; Starvaggi Cucuzza, Laura; Biolatti, Bartolomeo; Cannizzo, Francesca Tiziana

    2017-01-01

    Background The endocrinology of skeletal muscle is highly complex and many issues about hormone action in skeletal muscle are still unresolved. Aim of the work is to improve our knowledge on the relationship between skeletal muscle and 17β-estradiol. Methods The skeletal muscle cell line C2C12 was treated with 17β-estradiol, the oxytocin peptide and a combination of the two hormones. The mRNA levels of myogenic regulatory factors, myosin heavy chain, oxytocin, oxytocin receptor and adipogenic factors were analysed in C2C12 myotubes. Results It was demonstrated that C2C12 myoblasts and myotubes express oxytocin and its receptor, in particular the receptor levels physiologically increase in differentiated myotubes. Myotubes treated with 17β-estradiol overexpressed oxytocin and oxytocin receptor genes by approximately 3- and 29-fold, respectively. A decrease in the expression of fatty acid binding protein 4 (0.62-fold), a fat metabolism-associated gene, was observed in oxytocin-treated myotubes. On the contrary, fatty acid binding protein 4 was upregulated (2.66-fold) after the administration of the combination of 17β-estradiol and oxytocin. 17β-estradiol regulates oxytocin and its receptor in skeletal muscle cells and they act in a synergic way on fatty acid metabolism. Discussion Oxytocin and its receptor are physiologically regulated along differentiation. 17β-estradiol regulates oxytocin and its receptor in skeletal muscle cells. 17β-estradiol and oxytocin act in a synergic way on fatty acid metabolism. A better understanding of the regulation of skeletal muscle homeostasis by estrogens and oxytocin peptide could contribute to increase our knowledge of muscle and its metabolism. PMID:28382233

  11. Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts

    SciTech Connect

    Watanabe, Tomonobu M.; Higuchi, Sayaka; Kawauchi, Keiko; Tsukasaki, Yoshikazu; Ichimura, Taro; Fujita, Hideaki

    2012-02-24

    Highlights: Black-Right-Pointing-Pointer Change in the epigenetic landscape during myogenesis was optically investigated. Black-Right-Pointing-Pointer Mobility of nuclear proteins was used to state the epigenetic status of the cell. Black-Right-Pointing-Pointer Mobility of nuclear proteins decreased as myogenesis progressed in C2C12. Black-Right-Pointing-Pointer Differentiation state diagram was developed using parameters obtained. -- Abstract: Skeletal muscle undergoes complicated differentiation steps that include cell-cycle arrest, cell fusion, and maturation, which are controlled through sequential expression of transcription factors. During muscle differentiation, remodeling of the epigenetic landscape is also known to take place on a large scale, determining cell fate. In an attempt to determine the extent of epigenetic remodeling during muscle differentiation, we characterized the plasticity of the chromatin structure using C2C12 myoblasts. Differentiation of C2C12 cells was induced by lowering the serum concentration after they had reached full confluence, resulting in the formation of multi-nucleated myotubes. Upon induction of differentiation, the nucleus size decreased whereas the aspect ratio increased, indicating the presence of force on the nucleus during differentiation. Movement of the nucleus was also suppressed when differentiation was induced, indicating that the plasticity of chromatin changed upon differentiation. To evaluate the histone dynamics during differentiation, FRAP experiment was performed, which showed an increase in the immobile fraction of histone proteins when differentiation was induced. To further evaluate the change in the histone dynamics during differentiation, FCS was performed, which showed a decrease in histone mobility on differentiation. We here show that the plasticity of chromatin decreases upon differentiation, which takes place in a stepwise manner, and that it can be used as an index for the differentiation stage

  12. A Report of the Study Group on Affirmative Action to the Committee on Education and Labor. U.S. House of Representatives. 100th Congress, First Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    Equal employment opportunity (EEO) and affirmative action policies have been the subject of much debate in recent years. Much of the disagreement is based on incomplete or distorted views about what affirmative action is and how it affects minorities and nonminorities. The Study Group on Affirmative Action was created to investigate current EEO…

  13. Clarification of the Three-Body Decay of 12C (12.71MeV)

    NASA Astrophysics Data System (ADS)

    Fynbo, H. O.; Prezado, Y.; Bergmann, U. C.; Borge, M. J.; Dendooven, P.; Huang, W. X.; Huikari, J.; Jeppesen, H.; Jones, P.; Jonson, B.; Meister, M.; Nyman, G.; Riisager, K.; Tengblad, O.; Vogelius, I. S.; Wang, Y.; Weissman, L.; Rolander, K. Wilhelmsen; Äystö, J.

    2003-08-01

    Using β decays of a clean source of 12N produced at the IGISOL facility, we have measured the breakup of the 12C (12.71MeV) state into three α particles with a segmented particle detector setup. The high quality of the data permits solving the question of the breakup mechanism of the 12.71MeV state, a longstanding problem in few-body nuclear physics. Among existing models, a modified sequential model fits the data best, but systematic deviations indicate that a three-body description is needed.

  14. C12/C13-ratio determination in nanodiamonds by atom-probe tomography.

    PubMed

    Lewis, Josiah B; Isheim, Dieter; Floss, Christine; Seidman, David N

    2015-12-01

    The astrophysical origins of ∼ 3 nm-diameter meteoritic nanodiamonds can be inferred from the ratio of C12/C13. It is essential to achieve high spatial and mass resolving power and minimize all sources of signal loss in order to obtain statistically significant measurements. We conducted atom-probe tomography on meteoritic nanodiamonds embedded between layers of Pt. We describe sample preparation, atom-probe tomography analysis, 3D reconstruction, and bias correction. We present new data from meteoritic nanodiamonds and terrestrial standards and discuss methods to correct isotopic measurements made with the atom-probe tomograph. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. Maryalnd House enironment & Transportation Committee Visit

    NASA Image and Video Library

    2016-11-15

    Center Director Chris Scolese welcomed the Maryland House Enironment & Tranportation Committee to Goddard on November 15, 2016. The group visiited the James Webb Space Telescope JWST and saw the mirrors open, then they toured the Robotics Operations Facility.

  16. 77 FR 63831 - Consumer Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-17

    ... political advertising. The Committee may also consider other recommendations from its working groups, and... @fcc or via the Commission's Facebook page at www.facebook.com/fcc . Alternatively, written comments...

  17. 11 CFR 9002.9 - Political committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... means any committee, club, association, organization or other group of persons (whether or not... to influence, the election of any candidate to the office of President or Vice President of the...

  18. Maryland House Environment & Transportation Committee Visit

    NASA Image and Video Library

    2016-11-15

    Center Director Chris Scolese welcomed the Maryland House Environment & Transportation Committee to Goddard on November 15, 2016. The group visited the James Webb Space Telescope JWST and then they toured the Robotics Operations Facility.

  19. Maryalnd House enironment & Transportation Committee Visit

    NASA Image and Video Library

    2016-11-15

    Center Director Chris Scolese welcomed the Maryland House Environment & Transportation Committee to Goddard on November 15, 2016. The group visited the James Webb Space Telescope JWST, then they toured the Robotics Operations Facility.

  20. Maryland House Environment & Transportation Committee Visit

    NASA Image and Video Library

    2016-11-15

    Center Director Chris Scolese welcomed the Maryland House Environment & Transportation Committee to Goddard on November 15, 2016. The group visited the James Webb Space Telescope JWST and saw the mirrors open, then they toured the Robotic Operations Center - ROC.

  1. CCCT - NCTN Steering Committees - Gynecologic Cancers

    Cancer.gov

    The Gynecologic Cancers Steering Committee evaluates and prioritizes concepts for phase 2 and 3 clinical trials in adult gynecologic cancers. The GCSC is also intent on fostering collaboration with international groups and institutions conducting trials.

  2. 78 FR 4120 - Recreation Resource Advisory Committees

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-18

    ... 10(e) and (f) of the Federal Advisory Committee Act (5 U.S.C. App. II). Equal opportunity practices..., individuals with demonstrated ability to represent women, men, racial and ethnic groups, and persons with...

  3. 76 FR 41667 - Airworthiness Directives; Hawker Beechcraft Corporation Models B300 and B300C (C-12W) Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF TRANSPORTATION... Corporation Models B300 and B300C (C-12W) Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... Corporation Models B300 and B300C (C-12W) airplanes. This AD was prompted by an error found in the...

  4. [Care ethics committees].

    PubMed

    Abel, F

    2006-01-01

    The author, who in 1976 started the first Healthcare Ethics Committees in Spain, analyses the advantages of being able to count, from the outset, on a moral community of strongly motivated health professionals at a public, university, maternal-infant hospital of reference, privately managed by the Order of San Juan de Dios. From his perspective, he considers that the evolution of these committees shows patterns of similar conflicts in overcoming the ethical-philosophical conflicts of scientific and ethical-religious reductionisms. He considers that only interdisciplinary and transdisciplinary dialogue makes it possible, in our plural society, to find--through deliberation--the best route for solving ethical conflicts respecting the dignity of the patients and the health professionals. He opted for an autonomous ethics in a context of faith. He considers it to be an error to replace interdisciplinary dialogue within the hospital with consulting groups or persons specialising in professional ethics, the self-denominated "bioethical consultants", whether they be doctors or lawyers. He is very critical of the widespread error of confusing criteria of positive evaluation of the "Joint Commission" with criteria of careful deliberation, and he schematises models for analysing problems and placing them in context.

  5. Dose calculation formalisms and consensus dosimetry parameters for intravascular brachytherapy dosimetry: Recommendations of the AAPM Therapy Physics Committee Task Group No. 149

    SciTech Connect

    Chiu-Tsao, Sou-Tung; Schaart, Dennis R.; Soares, Christopher G.; Nath, Ravinder

    2007-11-15

    Since the publication of AAPM Task Group 60 report in 1999, a considerable amount of dosimetry data for the three coronary brachytherapy systems in use in the United States has been reported. A subgroup, Task Group 149, of the AAPM working group on Special Brachytherapy Modalities (Bruce Thomadsen, Chair) was charged to develop recommendations for dose calculation formalisms and the related consensus dosimetry parameters. The recommendations of this group are presented here. For the Cordis {sup 192}Ir and Novoste {sup 90}Sr/{sup 90}Y systems, the original TG-43 formalism in spherical coordinates should be used along with the consensus values of the dose rate constant, geometry function, radial dose function, and anisotropy function for the single seeds. Contributions from the single seeds should be added linearly for the calculation of dose distributions from a source train. For the Guidant {sup 32}P wire system, the modified TG-43 formalism in cylindrical coordinates along with the recommended data for the 20 and 27 mm wires should be used. Data tables for the 6, 10, 14, 18, and 22 seed trains of the Cordis system, 30, 40, and 60 mm seed trains of the Novoste system, and the 20 and 27 mm wires of the Guidant system are presented along with our rationale and methodology for selecting the consensus data. Briefly, all available datasets were compared with each other and the consensus dataset was either an average of available data or the one obtained from the most densely populated study; in most cases this was a Monte Carlo calculation.

  6. CCITT (International Telegraph Consultative Committee) Study Groups XI and XVIII Draft Recommendations for ISDN (Integrated Services Digital Networks), November 8-December 2, 1983.

    DTIC Science & Technology

    1983-12-01

    Electromagnetic interference radiation from ISDN wiring is apt to be fairly significant unless the system is carefully balanced around ground and the...next is received. Each group of bits that might be transmitted from one terminal, such as eight B channel bits, is followed by a balancing bit, called...an L bit, that insures that the d.c. balance is restored to the bus. Since logical Os are alternating pulses, there can never be more than one bit

  7. Possibility of Autocrine β-Adrenergic Signaling in C2C12 Myotubes

    PubMed Central

    SMITH, JILL L.; PATIL, PANKAJ B.; MINTEER, SHELLEY D.; LIPSITZ, JASON R.; FISHER, JONATHAN S.

    2005-01-01

    Levodopa reportedly inhibits insulin action in skeletal muscle. Here we show that C2C12 myotubes produce levodopa and that insulin-stimulated glucose transport is enhanced when endogenous levodopa is depleted. Exogenous levodopa prevented the stimulation of glucose transport by insulin (P < 0.05) and increased cAMP concentrations (P < 0.05). The decrease in insulin-stimulated glucose transport caused by levodopa was attenuated by propranolol (a β-adrenergic antagonist) and prevented by NSD-1015 (NSD), an inhibitor of DOPA decarboxylase (DDC; converts levodopa to dopamine). Propranolol and NSD both prevented levodopa-related increases in [cAMP]. However, the effects of levodopa were unlikely to be dependent on the conversion of levodopa to catecholamines because we could detect neither DDC in myotubes nor catecholamines in media after incubation of myotubes with levodopa. The data suggest the possibility of novel autocrine β-adrenergic action in C2C12 myotubes in which levodopa, produced by myotubes, could have hormone-like effects that impinge on glucose metabolism. PMID:16339749

  8. Polyphenols prevent clinorotation-induced expression of atrogenes in mouse C2C12 skeletal myotubes.

    PubMed

    Hemdan, Dalia Ismaeil Ibrahim; Hirasaka, Katsuya; Nakao, Reiko; Kohno, Shohei; Kagawa, Sachiko; Abe, Tomoki; Harada-Sukeno, Akiko; Okumura, Yuushi; Nakaya, Yutaka; Terao, Junji; Nikawa, Takeshi

    2009-02-01

    Oxidative stress is a key factor in stimulating the expression of atrogenes, which are muscle atrophy-related ubiquitin ligases, in skeletal muscle, and it induces muscle atrophy during unloading. However, the effects of antioxidative nutrients on atrogene expression have not been demonstrated. We report on the inhibitory effects of polyphenols, such as epicatechin (EC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) and quercetin, on atrogene expression up-regulated by three dimensional (3D)-clinorotation or glucocorticoid. These treatments markedly elevated the expression of atrogenes, including atrogin-1 and MuRF-1, in mouse C2C12 myoblasts and myotubes. Interestingly, EC, ECg, EGCg and quercetin significantly decreased the expression of atrogin-1 and MuRF-1 up-regulated by 3D-clinorotation, whereas they hardly affected atrogene expression induced by dexamethasone. ERK signaling is a well known MAPK pathway to mediate oxidative stress. Therefore, we also investigated the effect of these polyphenols on phosphorylation of ERK in C2C12 myotubes. As expected, EC, ECg, EGCg, and quercetin significantly suppressed phosphorylation of ERK, corresponding to the up-regulation of atrogenes induced by 3D-clinorotation. These results suggest that antioxidative nutrients, such as catechins and quercetin, suppress atrogene expression in skeletal muscle cells, possibly through the inhibition of ERK signaling. Thus, catechins and quercetin may prevent unloading-mediated muscle atrophy.

  9. Electrical Impedance Monitoring of C2C12 Myoblast Differentiation on an Indium Tin Oxide Electrode

    PubMed Central

    Park, Ilhwan; Hong, Yeonhee; Jun, Young-Hoo; Lee, Ga-Yeon; Jun, Hee-Sook; Pyun, Jae-Chul; Choi, Jeong-Woo; Cho, Sungbo

    2016-01-01

    Electrical cell-substrate impedance sensing is increasingly being used for label-free and real-time monitoring of changes in cell morphology and number during cell growth, drug screening, and differentiation. In this study, we evaluated the feasibility of using ECIS to monitor C2C12 myoblast differentiation using a fabricated indium tin oxide (ITO) electrode-based chip. C2C12 myoblast differentiation on the ITO electrode was validated based on decreases in the mRNA level of MyoD and increases in the mRNA levels of myogenin and myosin heavy chain (MHC). Additionally, MHC expression and morphological changes in myoblasts differentiated on the ITO electrode were comparable to those in cells in the control culture dish. From the monitoring the integration of the resistance change at 21.5 kHz, the cell differentiation was label-free and real-time detectable in 30 h of differentiation (p < 0.05). PMID:27929401

  10. Ferroelectric response in an achiral non-symmetric bent liquid crystal:C12C10

    NASA Astrophysics Data System (ADS)

    Subrahmanyam, S. V.; Chalapathi, P. V.; Mahabaleshwara, S.; Srinivasulu, M.; George, A. K.; Potukuchi, D. M.

    2014-10-01

    An achiral Non-Symmetric Bent Liquid Crystal (BLC) with a Oxadiazole based hetero cyclic central moiety, abbreviated as C12C10 viz., dodecyl[4-{5-(4‧-decyloxy)biphenyl-4-yl}-1,2,4-oxadiazol-3-yl]benzoate, exhibiting FerroElectric (FE) response is reported. Product is confirmed by 1H NMR, 13C NMR and elemental analysis. Characterization of BLC phases is carried out by Polarized Optical Microscopy (POM), Differential Scanning Calorimetry (DSC), Spontaneous Polarization (PS) and Low Frequency (10 Hz-10 MHz) Dielectric Relaxation studies. C12C10 exhibits enantiotropic LC SmA, FE B2, SmG, SmE phase variance. I-SmA, B2-SmG and SmG-SmE transitions are of first order nature. FE B2 phases exhibits a moderate PS of 80 nC cm-2. B2 phase exhibits Curie-Weiss behavior to confirm FE nature. Off-centered low frequency (KHz) dispersion infers a scissor mode and a high frequency (MHz) mode to reflect the distinct time-scale response. Dielectric Dispersion is relatively susceptible in lower frequency KHz region. Arrhenius shift in Relaxation Frequency (fR) infers higher activation energy (Ea) in non-FE phases for HF mode and lower value for KHz mode. Trends of fR, dielectric strength Δε, α-parameter and Ea are discussed in view of the data reported in other LC compounds.

  11. Theoretical investigation of stabilities and optical properties of Si12C12 clusters

    NASA Astrophysics Data System (ADS)

    Duan, Xiaofeng F.; Burggraf, Larry W.

    2015-01-01

    By sorting through hundreds of globally stable Si12C12 isomers using a potential surface search and using simulated annealing, we have identified low-energy structures. Unlike isomers knit together by Si-C bonds, the lowest energy isomers have segregated carbon and silicon regions that maximize stronger C-C bonding. Positing that charge separation between the carbon and silicon regions would produce interesting optical absorption in these cluster molecules, we used time-dependent density functional theory to compare the calculated optical properties of four isomers representing structural classes having different types of silicon and carbon segregation regions. Absorptions involving charge transfer between segregated carbon and silicon regions produce lower excitation energies than do structures having alternating Si-C bonding for which frontier orbital charge transfer is exclusively from separated carbon atoms to silicon atoms. The most stable Si12C12 isomer at temperatures below 1100 K is unique as regards its high symmetry and large optical oscillator strength in the visible blue. Its high-energy and low-energy visible transitions (1.15 eV and 2.56 eV) are nearly pure one-electron silicon-to-carbon transitions, while an intermediate energy transition (1.28 eV) is a nearly pure carbon-to-silicon one-electron charge transfer.

  12. Theoretical investigation of stabilities and optical properties of Si12C12 clusters.

    PubMed

    Duan, Xiaofeng F; Burggraf, Larry W

    2015-01-21

    By sorting through hundreds of globally stable Si12C12 isomers using a potential surface search and using simulated annealing, we have identified low-energy structures. Unlike isomers knit together by Si-C bonds, the lowest energy isomers have segregated carbon and silicon regions that maximize stronger C-C bonding. Positing that charge separation between the carbon and silicon regions would produce interesting optical absorption in these cluster molecules, we used time-dependent density functional theory to compare the calculated optical properties of four isomers representing structural classes having different types of silicon and carbon segregation regions. Absorptions involving charge transfer between segregated carbon and silicon regions produce lower excitation energies than do structures having alternating Si-C bonding for which frontier orbital charge transfer is exclusively from separated carbon atoms to silicon atoms. The most stable Si12C12 isomer at temperatures below 1100 K is unique as regards its high symmetry and large optical oscillator strength in the visible blue. Its high-energy and low-energy visible transitions (1.15 eV and 2.56 eV) are nearly pure one-electron silicon-to-carbon transitions, while an intermediate energy transition (1.28 eV) is a nearly pure carbon-to-silicon one-electron charge transfer.

  13. Vibrational dynamics of a non-degenerate ultrafast rotor: The (C12,C13)-oxalate ion

    PubMed Central

    Kuroda, Daniel G.; Abdo, Mohannad; Chuntonov, Lev; Smith, Amos B.; Hochstrasser, Robin M.

    2013-01-01

    Molecular ions undergoing ultrafast conformational changes on the same time scale of water motions are of significant importance in condensed phase dynamics. However, the characterization of systems with fast molecular motions has proven to be both experimentally and theoretically challenging. Here, we report the vibrational dynamics of the non-degenerate (C12,C13)-oxalate anion, an ultrafast rotor, in aqueous solution. The infrared absorption spectrum of the (C12,C13)-oxalate ion in solution reveals two vibrational transitions separated by approximately 40 cm−1 in the 1500–1600 cm−1 region. These two transitions are assigned to vibrational modes mainly localized in each of the carboxylate asymmetric stretch of the ion. Two-dimensional infrared spectra reveal the presence and growth of cross-peaks between these two transitions which are indicative of coupling and population transfer, respectively. A characteristic time of sub-picosecond cross-peaks growth is observed. Ultrafast pump-probe anisotropy studies reveal essentially the same characteristic time for the dipole reorientation. All the experimental data are well modeled in terms of a system undergoing ultrafast population transfer between localized states. Comparison of the experimental observations with simulations reveal a reasonable agreement, although a mechanism including only the fluctuations of the coupling caused by the changes in the dihedral angle of the rotor, is not sufficient to explain the observed ultrafast population transfer. PMID:24182056

  14. Independent AMP and NAD signaling regulates C2C12 differentiation and metabolic adaptation.

    PubMed

    Hsu, Chia George; Burkholder, Thomas J

    2016-12-01

    The balance of ATP production and consumption is reflected in adenosine monophosphate (AMP) and nicotinamide adenine dinucleotide (NAD) content and has been associated with phenotypic plasticity in striated muscle. Some studies have suggested that AMPK-dependent plasticity may be an indirect consequence of increased NAD synthesis and SIRT1 activity. The primary goal of this study was to assess the interaction of AMP- and NAD-dependent signaling in adaptation of C2C12 myotubes. Changes in myotube developmental and metabolic gene expression were compared following incubation with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) and nicotinamide mononucleotide (NMN) to activate AMPK- and NAD-related signaling. AICAR showed no effect on NAD pool or nampt expression but significantly reduced histone H3 acetylation and GLUT1, cytochrome C oxidase subunit 2 (COX2), and MYH3 expression. In contrast, NMN supplementation for 24 h increased NAD pool by 45 % but did not reduce histone H3 acetylation nor promote mitochondrial gene expression. The combination of AMP and NAD signaling did not induce further metabolic adaptation, but NMN ameliorated AICAR-induced myotube reduction. We interpret these results as indication that AMP and NAD contribute to C2C12 differentiation and metabolic adaptation independently.

  15. Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts.

    PubMed

    Watanabe, Tomonobu M; Higuchi, Sayaka; Kawauchi, Keiko; Tsukasaki, Yoshikazu; Ichimura, Taro; Fujita, Hideaki

    2012-02-24

    Skeletal muscle undergoes complicated differentiation steps that include cell-cycle arrest, cell fusion, and maturation, which are controlled through sequential expression of transcription factors. During muscle differentiation, remodeling of the epigenetic landscape is also known to take place on a large scale, determining cell fate. In an attempt to determine the extent of epigenetic remodeling during muscle differentiation, we characterized the plasticity of the chromatin structure using C2C12 myoblasts. Differentiation of C2C12 cells was induced by lowering the serum concentration after they had reached full confluence, resulting in the formation of multi-nucleated myotubes. Upon induction of differentiation, the nucleus size decreased whereas the aspect ratio increased, indicating the presence of force on the nucleus during differentiation. Movement of the nucleus was also suppressed when differentiation was induced, indicating that the plasticity of chromatin changed upon differentiation. To evaluate the histone dynamics during differentiation, FRAP experiment was performed, which showed an increase in the immobile fraction of histone proteins when differentiation was induced. To further evaluate the change in the histone dynamics during differentiation, FCS was performed, which showed a decrease in histone mobility on differentiation. We here show that the plasticity of chromatin decreases upon differentiation, which takes place in a stepwise manner, and that it can be used as an index for the differentiation stage during myogenesis using the state diagram developed with the parameters obtained in this study.

  16. Measurement of the ^12C+^12C Fusion Reaction with MUSIC

    NASA Astrophysics Data System (ADS)

    Carnelli, P. F. F.; Almaraz-Calderon, S.; Henderson, D.; Rehm, K. E.; Albers, M.; Alcorta, M.; Bertone, P. F.; Esbensen, H.; Fernandez-Niello, J. O.; Jiang, C. L.; Lighthall, J. C.; Marley, S. T.; Palchan-Hazan, T.; Pardo, R. C.; Paul, M.

    2012-10-01

    The fusion of the ^12C+^12C system is of great interest in nuclear structure and nuclear astrophysics. Above the Coulomb barrier, the excitation function of this system exhibits oscillations, which are not well understood. There is also a significant discrepancy between the experimental fusion cross-section and recent coupled-channel calculations that is not present in other carbon systems. To address these issues, we have re-measured the fusion excitation function for ^12,13C+^12C in the energy range of 10 MeV < Ecm < 20 MeV using a Multi-Sampling Ionization Chamber (MUSIC) detector. The gas of the ionization chamber (CH4) served as both the target material and the counter gas. One of the main advantages of this method is that the excitation function is measured over a large range of energies using only one beam energy. This method has been proven to be successful and it will be used to measure fusion reactions in other light systems. The experimental results will be presented and compared to previous experimental data and theoretical models.

  17. PUGNAc induces protein ubiquitination in C2C12 myotube cells.

    PubMed

    Park, Ja-Hye; Lee, Jeong-Eun; Moon, Pyong-Gon; Baek, Moon-Chang

    2015-12-01

    O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) regulates many cellular processes including the cell cycle, cell signaling, and protein trafficking. Dysregulation of O-GlcNAcylation may be involved in the development of insulin resistance and type 2 diabetes. Therefore, it is necessary to identify cellular proteins that are induced by elevated O-GlcNAcylation. Here, using adenosine 5'-triphosphate affinity chromatography, we employed a proteomic approach in order to identify differentially expressed proteins in response to treatment with the O-GlcNAcase inhibitor, O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino-N-phenylcarbamate (PUGNAc), in mouse C2C12 myotube cells. Among 205 selected genes, we identified 68 nucleotide-binding proteins, 14 proteins that have adenosinetriphosphatase activity, and 10 proteins with ligase activity. Upregulation of proteins, including ubiquitin-activating enzyme E1, proteasome subunit 20S, cullin-associated NEDD8-dissociated protein 1, ezrin, and downregulation of the protein nucleoside diphosphate kinase B, were confirmed by western blot analysis. In particular, we found that the protein ubiquitination level in C2C12 cells was increased by PUGNAc treatment. This is the first report of quantitative proteomic profiles of myotube cells after treatment with PUGNAc, and our results demonstrate the potential to enhance understanding of the relationship between insulin resistance, O-GlcNAc, and PUGNAc in the future. Copyright © 2015 John Wiley & Sons, Ltd.

  18. NASA science committee appointments

    NASA Astrophysics Data System (ADS)

    Zielinski, Sarah

    2006-10-01

    NASA Administrator Michael Griffin has made three new appointments to the NASA Advisory Council's (NAC' Science Committee, NASA announced on 22 September. Edward David, president of EED, Inc., and science advisor to the President from 1970 to 1973, will serve as the committee-s chair. Also appointed to the committee were Owen Garriott, a retired scientist astronaut, and Alan Stern, executive director of the Space Science and Engineering Division of the Southwest Research Institute (San Antonio, Tex.). David, Garriott, and Stern-who are among nine new members of the full advisory committee that were announced on 22 September-will replace three members of the Science Committee who resigned in August: Science Committee Chair Charles Kennel (Scripps Institution of Oceanography), Wesley Huntress (Carnegie Institution of Washington), and Eugene Levy (Rice University). The NAC's next public meeting will be held on 12 October at Goddard Space Flight Center in Greenbelt, Md.

  19. 42 CFR 456.206 - Organization and composition of UR committee; disqualification from UR committee membership.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... UTILIZATION CONTROL Utilization Control: Mental Hospitals Ur Plan: Administrative Requirements § 456.206... the diagnosis and treatment of mental diseases, and assisted by other professional personnel. (c) The UR committee must be constituted as— (1) A committee of the mental hospital staff; (2) A group...

  20. 42 CFR 456.206 - Organization and composition of UR committee; disqualification from UR committee membership.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... UTILIZATION CONTROL Utilization Control: Mental Hospitals Ur Plan: Administrative Requirements § 456.206... the diagnosis and treatment of mental diseases, and assisted by other professional personnel. (c) The UR committee must be constituted as— (1) A committee of the mental hospital staff; (2) A group...

  1. 42 CFR 456.206 - Organization and composition of UR committee; disqualification from UR committee membership.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... UTILIZATION CONTROL Utilization Control: Mental Hospitals Ur Plan: Administrative Requirements § 456.206... the diagnosis and treatment of mental diseases, and assisted by other professional personnel. (c) The UR committee must be constituted as— (1) A committee of the mental hospital staff; (2) A group...

  2. Accuracy and calibration of integrated radiation output indicators in diagnostic radiology: A report of the AAPM Imaging Physics Committee Task Group 190

    SciTech Connect

    Lin, Pei-Jan P.; Schueler, Beth A.; Balter, Stephen; Strauss, Keith J.; Wunderle, Kevin A.; LaFrance, M. Terry; Kim, Don-Soo; Behrman, Richard H.; Shepard, S. Jeff; Bercha, Ishtiaq H.

    2015-12-15

    Due to the proliferation of disciplines employing fluoroscopy as their primary imaging tool and the prolonged extensive use of fluoroscopy in interventional and cardiovascular angiography procedures, “dose-area-product” (DAP) meters were installed to monitor and record the radiation dose delivered to patients. In some cases, the radiation dose or the output value is calculated, rather than measured, using the pertinent radiological parameters and geometrical information. The AAPM Task Group 190 (TG-190) was established to evaluate the accuracy of the DAP meter in 2008. Since then, the term “DAP-meter” has been revised to air kerma-area product (KAP) meter. The charge of TG 190 (Accuracy and Calibration of Integrated Radiation Output Indicators in Diagnostic Radiology) has also been realigned to investigate the “Accuracy and Calibration of Integrated Radiation Output Indicators” which is reflected in the title of the task group, to include situations where the KAP may be acquired with or without the presence of a physical “meter.” To accomplish this goal, validation test protocols were developed to compare the displayed radiation output value to an external measurement. These test protocols were applied to a number of clinical systems to collect information on the accuracy of dose display values in the field.

  3. Cartap-induced cytotoxicity in mouse C2C12 myoblast cell line and the roles of calcium ion and oxidative stress on the toxic effects.

    PubMed

    Liao, Jiunn-Wang; Kang, Jaw-Jou; Jeng, Chian-Ren; Chang, Shao-Kuang; Kuo, Ming-Jang; Wang, Shun-Cheng; Liu, Michael R S; Pang, Victor Fei

    2006-02-15

    Our previous study has demonstrated that instead of neuromuscular blockage cartap, an organonitrogen insecticide, could cause a marked irreversible Ca2+-dependent contracture in both isolated mouse and rabbit phrenic nerve-diaphragms. We further examined the potential of direct myocytotoxicity of cartap and the possible roles of calcium ion and oxidative stress on cartap-induced muscle cell injury using the mouse myoblast cell line, C2C12. Cartap exerted a dose- and time-dependent cytotoxic effect in C2C12 cells measured by MTT colorimetric assay and trypan blue dye exclusion. The extracellular activities of both creatine kinase (CK) and lactate dehydrogenase (LDH) were elevated in the cartap-treated groups at or greater than 100 microM. The isoenzymatic profiles showed that the elevations were mainly due to CK-3, LDH-3, and LDH-4. Following the addition of 0.5-2.5mM EGTA, a Ca2+ chelator, or 30-100 microM verapamil, an L-type Ca2+ channel blocker, the cartap-induced reduction in MTT metabolic rate of C2C12 cells was significantly restored in a dose-dependent manner in both EGTA and verapamil-treated cells. Furthermore, EGTA could significantly reduce the cartap-induced elevation in the levels of total extracellular CK and LDH activities. Additionally, cartap significantly increased the level of endogenous reactive oxygen species (ROS) in C2C12 cells in a dose- and time-dependent manner. The cartap-induced ROS generation could be significantly inhibited by antioxidants, including Vitamins C and E, catalase, and superoxide dismutase, with catalase the most effective. EGTA could significantly inhibit cartap-induced ROS generation in a dose-dependent manner. The results suggested that cartap could induce ROS generation in C2C12 cells via a Ca2+-dependent mechanism resulting in subsequent cytotoxicity, at least partially, to C2C12 cells. It is speculated that both Ca2+ and Ca2+-induced ROS may also play the central role on the myogenic contracture and myofiber injury

  4. Photobiomodulation Protects and Promotes Differentiation of C2C12 Myoblast Cells Exposed to Snake Venom

    PubMed Central

    da Silva, Aline; Vieira, Rodolfo Paula; Mesquita-Ferrari, Raquel Agnelli; Cogo, José Carlos; Zamuner, Stella Regina

    2016-01-01

    Background Snakebites is a neglected disease and in Brazil is considered a serious health problem, with the majority of the snakebites caused by the genus Bothrops. Antivenom therapy and other first-aid treatments do not reverse local myonecrose which is the main sequel caused by the envenomation. Several studies have shown the effectiveness of low level laser (LLL) therapy in reducing local myonecrosis induced by Bothropic venoms, however the mechanism involved in this effect is unknown. In this in vitro study, we aimed to analyze the effect of LLL irradiation against cytotoxicity induced by Bothrops jararacussu venom on myoblast C2C12 cells. Methodology C2C12 were utilized as a model target and were incubated with B. jararacussu venom (12.5 μg/mL) and immediately irradiated with LLL at wavelength of red 685 nm or infrared 830 nm with energy density of 2.0, 4.6 and 7.0 J/cm2. Effects of LLL on cellular responses of venom-induced cytotoxicity were examined, including cell viability, measurement of cell damage and intra and extracellular ATP levels, expression of myogenic regulatory factors, as well as cellular differentiation. Results In non-irradiated cells, the venom caused a decrease in cell viability and a massive release of LDH and CK levels indicating myonecrosis. Infrared and red laser at all energy densities were able to considerably decrease venom-induced cytotoxicity. Laser irradiation induced myoblasts to differentiate into myotubes and this effect was accompanied by up regulation of MyoD and specially myogenin. Moreover, LLL was able to reduce the extracellular while increased the intracellular ATP content after venom exposure. In addition, no difference in the intensity of cytotoxicity was shown by non-irradiated and irradiated venom. Conclusion LLL irradiation caused a protective effect on C2C12 cells against the cytotoxicity caused by B. jararacussu venom and promotes differentiation of these cells by up regulation of myogenic factors. A modulatory

  5. Actin-associated protein palladin is required for migration behavior and differentiation potential of C2C12 myoblast cells

    SciTech Connect

    Nguyen, Ngoc Uyen Nhi; Liang, Vincent Roderick; Wang, Hao-Ven

    2014-09-26

    Highlights: • Palladin is involved in myogenesis in vitro. • Palladin knockdown by siRNA increases myoblast proliferation, viability and differentiation. • Palladin knockdown decreases C2C12 myoblast migration ability. - Abstract: The actin-associated protein palladin has been shown to be involved in differentiation processes in non-muscle tissues. However, but its function in skeletal muscle has rarely been studied. Palladin plays important roles in the regulation of diverse actin-related signaling in a number of cell types. Since intact actin-cytoskeletal remodeling is necessary for myogenesis, in the present study, we pursue to investigate the role of actin-associated palladin in skeletal muscle differentiation. Palladin in C2C12 myoblasts is knocked-down using specific small interfering RNA (siRNA). The results show that down-regulation of palladin decreased migratory activity of mouse skeletal muscle C2C12 myoblasts. Furthermore, the depletion of palladin enhances C2C12 vitality and proliferation. Of note, the loss of palladin promotes C2C12 to express the myosin heavy chain, suggesting that palladin has a role in the modulation of C2C12 differentiation. It is thus proposed that palladin is required for normal C2C12 myogenesis in vitro.

  6. Pseudomonas aeruginosa auto inducer3-oxo-C12-HSL exerts bacteriostatic effect and inhibits Staphylococcus epidermidis biofilm.

    PubMed

    Singh, Pradeep Kumar; Yadav, Vivek Kumar; Kalia, Manmohit; Dohare, Suhaga; Sharma, Deepmala; Agarwal, Vishnu

    2017-09-01

    Pseudomonas aeruginosa has evolved the 3-oxo-C12-HSL and C4-HSL based quorum sensing system which is responsible for the regulation of various virulence factors and helps to dominates over other bacterial species. Staphylococcus epidermidis has frequently been reported with P. aeruginosa while the role of C4-HSL and 3-oxo-C12-HSL on the S. epidermidis had widely been unexplored, and as per our knowledge, this is the first report on the impact of C4-HSL and 3-oxo-C12-HSL overS. epidermidis growth and biofilm. We found that among the two AHL molecules; only 3-oxo-C12-HSL was able to exert a significant effect in all the experiments including growth and biofilm of S. epidermidis. 3-oxo-C12-HSL at 100 μM and 200 μM concentrations were able to initiate the apparent transient type of planktonic growth inhibition in S. epidermidis. Microscopic analysis and biofilm quantification assay showed the inhibitory effect of 3-oxo-C12-HSL against S. epidermidis biofilm, initial attachment, and EPS production. The study concludes that P. aeruginosa associated 3-oxo-C12-HSL exerts the inhibitory effect on S. epidermidis growth and biofilms and thus it may also help Pseudomonasto dominate under the co-infection conditions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Design and development of pH-responsive HSPC:C12H25-PAA chimeric liposomes.

    PubMed

    Naziris, Nikolaos; Pippa, Natassa; Meristoudi, Anastasia; Pispas, Stergios; Demetzos, Costas

    2017-06-01

    The application of stimuli-responsive medical practices has emerged, in which pH-sensitive liposomes figure prominently. This study investigates the impact of the incorporation of different amounts of pH-sensitive polymer, C12H25-PAA (poly(acrylic acid) with a hydrophobic end group) in l-α-phosphatidylcholine, hydrogenated (Soy) (HSPC) phospholipidic bilayers, with respect to biomimicry and functionality. PAA is a poly(carboxylic acid) molecule, classified as a pH-sensitive polymer, whose pH-sensitivity is attributed to its regulative -COOH groups, which are protonated under acidic pH (pKa ∼4.2). Our concern was to fully characterize, in a biophysical and thermodynamical manner, the mixed nanoassemblies arising from the combination of the two biomaterials. At first, we quantified the physicochemical characteristics and physical stability of the prepared chimeric nanosystems. Then, we studied their thermotropic behavior, through measurement of thermodynamical parameters, using Differential Scanning Calorimetry (DSC). Finally, the loading and release of indomethacin (IND) were evaluated, as well as the physicochemical properties and stability of the nanocarriers incorporating it. As expected, thermodynamical findings are in line with physicochemical results and also explain the loading and release profiles of IND. The novelty of this investigation is the utilization of these pH-sensitive chimeric advanced Drug Delivery nano Systems (aDDnSs) in targeted drug delivery which relies entirely on the biophysics and thermodynamics between such designs and the physiological membranes and environment of living organisms.

  8. Nuclear co-repressor (NCoR) is required to maintain insulin sensitivity in C2 C12 myotubes.

    PubMed

    Choudhary, Abhijeet K; Dey, Chinmoy S

    2017-02-01

    Nuclear co-repressor (NCoR) regulates peripheral insulin sensitivity; however, its role in modulating insulin sensitivity in skeletal muscle remains elusive. Present study investigated protein expression and effect of NCoR on insulin sensitivity in murine skeletal muscle cell line C2 C12 . Myotubes as compared to myoblasts of C2 C12 cells were found to be more sensitive in response to insulin as increase in insulin-stimulated phosphorylation of AKT at serine 473 residue (pAKT(S473) ) was significantly higher in myotubes. Incidentally, reduced protein level of NCoR coincided with differentiation of myoblasts into myotubes of C2 C12 cells. However, insulin stimulation per se failed to affect protein level of NCoR either in myoblasts or myotubes of C2 C12 cells. To assess the role of NCoR on insulin sensitivity, NCoR was transiently knocked down using siRNA in myotubes of C2 C12 . In fact, transient silencing of NCoR led to significant reduction in insulin-stimulated pAKT(S473) and impaired glucose uptake. This observation is in contrast to published studies where NCoR has been reported to negatively regulate insulin signaling cascade. Furthermore, transient silencing of NCoR failed to improve insulin sensitivity in chronic hyperinsulinemia-induced insulin-resistant model of C2 C12 cells. Importantly, inhibition of lysosomal protein degradation pathway using ammonium chloride restored protein level of NCoR but failed to increase glucose uptake in serum-starved C2 C12 myotubes. Collectively, data from present study show differential protein level of NCoR under different cell state (myoblast and myotubes) of C2 C12 cells and NCoR proves to be vital for maintaining insulin sensitivity in C2 C12 myotubes.

  9. 78 FR 28602 - Merchant Marine Personnel Advisory Committee: Intercessional Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-15

    ... Guard, DHS. ACTION: Notice of Federal advisory committee working group meeting. SUMMARY: A working group of the Merchant Marine Personnel Advisory Committee (MERPAC) will meet to work on Task Statement 83... working group will meet on June 11, 2013, and June 12, 2013, from 8 a.m. until 4 p.m. Please note that...

  10. 77 FR 66857 - Merchant Marine Personnel Advisory Committee: Intercessional Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-07

    ... on the issues to be considered by the work group, which are listed in the ``Agenda'' section below... Guard, DHS. ACTION: Notice of Federal Advisory Committee Working Group Meeting. SUMMARY: A working group of the Merchant Marine Personnel Advisory Committee (MERPAC) will meet to work on Task Statement...

  11. Functional fitting of interstitial brachytherapy dosimetry data recommended by the AAPM Radiation Therapy Committee Task Group 43. American Association of Physicists in Medicine.

    PubMed

    Furhang, E E; Anderson, L L

    1999-02-01

    This work was undertaken to expedite implementation of the AAPM Task Group 43 recommendations, which call for significant modifications in the way dose is calculated for interstitial sources of 192Ir, 125I, and 103Pd as well as significant changes in the dose rate constant for 125I sources. The TG43 recommendations include a new formalism for dose calculation at points defined by the radial distance, r, from the source center and the angle, theta, that such a radius makes with the source axis. For each source type, values are tabulated for the radial dose function, the anisotropy function, and the anisotropy factor. The TG43 report includes fitting functions for the radial dose function in the form of polynomials, which are poorly behaved outside the range of fitted data. No functions are offered for the anisotropy function data or the anisotropy factor data, both of which could profit from some smoothing by such functions. We have found a double exponential fit to the radial dose function that not only approximates the data adequately but also appropriately approaches zero for very large distances. The anisotropy function is conveniently fit with a form of type 1 - f(r,theta)cos(theta)e(cr), which is exactly 1 at theta=90 degrees and approaches 1 for large r (for c<0), where f(r,theta) is a selected polynomial in the two variables. The form chosen for the anisotropy factor was 1 - (a+br)e(cr), which appropriately approaches 1 for large r (and c<0). Functional fits of these types are expected to facilitate implementation of TG43 recommendations, in that they may be either incorporated into dose algorithms or used to generate lookup tables of either the x, y or the r, theta format.

  12. Cancer survivorship practices, services, and delivery: a report from the Children's Oncology Group (COG) nursing discipline, adolescent/young adult, and late effects committees.

    PubMed

    Eshelman-Kent, Debra; Kinahan, Karen E; Hobbie, Wendy; Landier, Wendy; Teal, Steve; Friedman, Debra; Nagarajan, Rajaram; Freyer, David R

    2011-12-01

    To describe survivorship services provided by the Children's Oncology Group (COG), an assessment of services was undertaken. Our overall aims were (1) to describe survivorship services, including the extent of services provided, resources (personnel, philanthropy, and research funding), billing practices, and barriers to care and 2) to describe models of care that are in use for childhood cancer survivors and adult survivors of childhood cancer. One hundred seventy-nine of 220 COG institutions (81%) completed an Internet survey in 2007. One hundred fifty-five (87%) reported providing survivorship care. Fifty-nine percent of institutions provide care for their pediatric population in specialized late effects programs. For adult survivors, 47% of institutions chose models of care, which included transitioning to adult providers for risk-based health care, while 44% of institutions keep survivors indefinitely at the treating institution (Cancer Center Based Model without Community Referral). Sixty-eight percent provide survivors with a copy of their survivorship care plan. Only 31% of institutions provide a detailed summary of results after each clinic visit, and 41% have a database to track survivor health outcomes. Minimal time required for initial and annual survivorship visits is estimated to be approximately 120 and 90 min, respectively. The most prevalent barriers to care were the lack of dedicated time for program development and a perceived insufficient knowledge on the part of the clinician receiving the transition referral. Not all COG institutions provide dedicated survivorship care, care plans, or have databases for tracking outcomes. Transitioning to adult providers is occurring within the COG. Survivorship care is time intensive.

  13. Thermal stability of hexaprismane C12H12 and octaprismane C16H16

    NASA Astrophysics Data System (ADS)

    Shostachenko, S. A.; Maslov, M. M.; Prudkovskii, V. S.; Katin, K. P.

    2015-05-01

    The results of quantum-mechanical calculations of elementary prismanes—hexaprismane C12H12 and octaprismane C16H16—have been presented. Their stability has been investigated in terms of the density functional theory and nonorthogonal tight-binding model, and the heights of potential barriers preventing isomerization and decay have been determined. It has been established based on the analysis of the molecular dynamics data and the hypersurface of the potential energy of these metastable compounds that hexaprismane and octaprismane have a rather high kinetic stability, which indicates the possibility of the formation of carbon polyprismanes for applications in microelectronics and nanoelectronics, power engineering, pharmaceutics, metrology, and information technologies.

  14. Novel 1alpha,25-dihydroxyvitamin D3 analogues with the side chain at C12.

    PubMed

    González-Avión, Xosé C; Mouriño, Antonio; Rochel, Natacha; Moras, Dino

    2006-03-09

    The plethora of actions of 1alpha,25(OH)2D3 in various systems suggested wide clinical applications of vitamin D nuclear receptor (VDR) ligands in treatments of inflammation, dermatological indication, osteoporosis, cancers, and autoimmune diseases. More than 3000 vitamin D analogues have been synthesized in order to reduce the calcemic side effects while maintaining the transactivation potency of the natural ligand. In light of the crystal structures of the vitamin D nuclear receptor (VDR), novel analogues of the hormone 1alpha,25(OH)2D3 with side chains attached to C-12 were synthesized via the convergent Wittig-Horner approach. Among the compounds studied, the analogue 2b showed the highest binding affinity for VDR and was the most potent at inducing VDR transcriptional activity in a transient transfection assay (20% of the transactivation activity of the natural ligand).

  15. Tadalafil alters energy metabolism in C2C12 skeletal muscle cells.

    PubMed

    Sabatini, Stefania; Sgrò, Paolo; Duranti, Guglielmo; Ceci, Roberta; Di Luigi, Luigi

    2011-01-01

    Phosphodiesterases (PDEs) are a family of enzymes that hydrolyze cyclic nucleotides, thereby modulating cell functions. Three highly selective PDE5 inhibitors (PDE5i), sildenafil, vardenafil and tadalafil, have been developed for treatment of erectile dysfunction (ED). Experimental evidence showed that chronic treatment with sildenafil PDE5i in a mouse model of diet-induced obesity and insulin resistance improved insulin action and decreased circulating fatty acid levels. It has recently been shown that healthy athletes use PDE5i as performance enhancers, hence in the present study we investigated whether the long-lasting PDE5i tadalafil influences energy metabolism in C2C12 skeletal muscle cells by evaluating lactate production, glucose consumption, and citrate synthase and 3-OH acyl CoA dehydrogenase activities. Our data demonstrate that tadalafil is able to modulate energy homeostasis in mouse skeletal muscle cells, depending on the treatment length and dose.

  16. 76 FR 42160 - Commercial Space Transportation Advisory Committee-Public Teleconference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-18

    ... Working Group of the Commercial Space Transportation Advisory Committee Teleconference. SUMMARY: Pursuant... is hereby given of a teleconference of the Space Transportation Operations Working Group (STOWG) of the Commercial Space Transportation Advisory Committee (COMSTAC). The teleconference will take...

  17. National Nutrition Policy Study--1974. Hearings Before the Select Committee on Nutrition and Human Needs of the United States Senate Ninety-Third Congress, Second Session. Part 3A--Appendix to Nutrition and Special Groups. Hearings Held Washington, D. C., June 20, 1974.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Select Committee on Nutrition and Human Needs.

    These hearings before the Panel on Nutrition and Special Groups of the Select Senate Committee on Nutrition and Human Needs were held from June 19 to June 21, 1974. During each of the three days of hearings, various witnesses submitted statements and other testimony to the panel, as well as reports and publications pertaining to their testimony.…

  18. National Nutrition Policy Study--1974. Hearings Before the Select Committee on Nutrition and Human Needs of the United States Senate Ninety-Third Congress, Second Session. Part 3A--Appendix to Nutrition and Special Groups. Hearings Held Washington, D. C., June 20, 1974.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Select Committee on Nutrition and Human Needs.

    These hearings before the Panel on Nutrition and Special Groups of the Select Senate Committee on Nutrition and Human Needs were held from June 19 to June 21, 1974. During each of the three days of hearings, various witnesses submitted statements and other testimony to the panel, as well as reports and publications pertaining to their testimony.…

  19. Reactive-Atom Scattering from Liquid Crystals at the Liquid-Vacuum Interface: [C12mim][BF4] and 4-Cyano-4'-Octylbiphenyl (8CB).

    PubMed

    Purcell, Simon M; Tesa-Serrate, Maria A; Marshall, Brooks C; Bruce, Duncan W; D'Andrea, Lucía; Costen, Matthew L; Slattery, John M; Smoll, Eric J; Minton, Timothy K; McKendrick, Kenneth G

    2016-10-04

    Two complementary approaches were used to study the liquid-vacuum interface of the liquid-crystalline ionic liquid 1-dodecyl-3-methylimidazolium tetrafluoroborate ([C12mim][BF4]) in the smectic A (SmA) and isotropic phases. O atoms with two distinct incident translational energies were scattered from the surface of [C12mim][BF4]. Angle-dependent time-of-flight distributions and OH yields, respectively, were recorded from high- and low-energy O atoms. There were no significant changes in the measurements using either approach, nor the properties derived from them, accompanying the transition from the SmA to the isotropic phase. This indicates that the surface structure of [C12mim][BF4] remains essentially unchanged across the phase boundary, implying that the bulk order and surface structure are not strongly correlated for this material. This effect is ascribed to the strong propensity for the outer surfaces of ionic liquids to be dominated by alkyl chains, over an underlying layer rich in anions and cation head groups, whether or not the bulk material is a liquid crystal. In a comparative study, the OH yield from the surface of the liquid crystal, 8CB, was found to be affected by the bulk order, showing a surprising step increase at the SmA-nematic transition temperature, whose origin is the subject of speculation.

  20. A practical synthesis of long-chain iso-fatty acids (iso-C12-C19) and related natural products.

    PubMed

    Richardson, Mark B; Williams, Spencer J

    2013-01-01

    A gram-scale synthesis of terminally-branched iso-fatty acids (iso-C12-C19) was developed commencing with methyl undec-10-enoate (methyl undecylenate) (for iso-C12-C14) or the C15 and C16 lactones pentadecanolide (for iso-C15-C17) and hexadecanolide (for iso-C18-C19). Central to the approaches outlined is the two-step construction of the terminal isopropyl group through addition of methylmagnesium bromide to the ester/lactones and selective reduction of the resulting tertiary alcohols. Thus, the C12, C17 and C18 iso-fatty acids were obtained in three steps from commercially-available starting materials, and the remaining C13-C16 and C19 iso-fatty acids were prepared by homologation or recursive dehomologations of these fatty acids or through intercepting appropriate intermediates. Highlighting the synthetic potential of the iso-fatty acids and various intermediates prepared herein, we describe the synthesis of the natural products (S)-2,15-dimethylpalmitic acid, (S)-2-hydroxy-15-methylpalmitic acid, and 2-oxo-14-methylpentadecane.

  1. In the long run...longitudinal studies of psychopathology in children. Committee on Child Psychology. Group for the Advancement of Psychiatry. Report no.143.

    PubMed

    1999-01-01

    Longitudinal studies are difficult to do well. Too short a study and the results may be meaningless. Too long a study and the subjects cannot be found, money runs out, and research methods become seriously out of date. Despite these problems, there have been some longitudinal studies done that have greatly advanced our understanding of the nature and the treatment of psychopathology in childhood. Without these studies, much less would be known about the clinical course of important disorders, the effects of treatments, and the various risk and protective factors. None of these studies has been perfect. Some longitudinal studies did not focus on quite the right questions, some produced contradictory results, and others produced results that were hard to interpret. What have we learned from the longitudinal studies reviewed in this Group for the Advancement of Psychiatry (GAP) report? Many of the things that we have learned have been surprising--even counterintuitive. Pre- and perinatal insults need not necessarily lead to serious consequences in later life. Premature infants, if raised in nondeprived settings, are not likely to be mentally retarded or learning disabled. Today, of course, premature infants who would not have been kept alive 15 years ago are surviving. Will this advancement led to an untoward outcome? We do not know. New longitudinal studies need to be done. Certain serious illnesses emerging later in childhood may be associated with a greater risk of psychopathology. This risk is true at least for those with asthma. Psychological factors, such as psychological stress, also may lead to exacerbation of asthmatic attacks. Whether other illnesses are associated with a greater risk of psychopathology simply has not been studied adequately. Infant temperamental characteristics can be classified and measured; however, they appear to predict little in terms of later personality development or psychopathology. Although temperamental characteristics measured

  2. Degree of Suppression of Mouse Myoblast Cell Line C2C12 Differentiation Varies According to Chondroitin Sulfate Subtype

    PubMed Central

    Warita, Katsuhiko; Oshima, Nana; Takeda-Okuda, Naoko; Tamura, Jun-ichi; Hosaka, Yoshinao Z.

    2016-01-01

    Chondroitin sulfate (CS), a type of glycosaminoglycan (GAG), is a factor involved in the suppression of myogenic differentiation. CS comprises two repeating sugars and has different subtypes depending on the position and number of bonded sulfate groups. However, the effect of each subtype on myogenic differentiation remains unclear. In this study, we spiked cultures of C2C12 myoblasts, cells which are capable of undergoing skeletal muscle differentiation, with one of five types of CS (CS-A, -B, -C, -D, or -E) and induced differentiation over a fixed time. After immunostaining of the formed myotubes with an anti-MHC antibody, we counted the number of nuclei in the myotubes and then calculated the fusion index (FI) as a measure of myotube differentiation. The FI values of all the CS-treated groups were lower than the FI value of the control group, especially the group treated with CS-E, which displayed notable suppression of myotube formation. To confirm that the sugar chain in CS-E is important in the suppression of differentiation, chondroitinase ABC (ChABC), which catabolizes CS, was added to the media. The addition of ChABC led to the degradation of CS-E, and neutralized the suppression of myotube formation by CS-E. Collectively, it can be concluded that the degree of suppression of differentiation depends on the subtype of CS and that CS-E strongly suppresses myogenic differentiation. We conclude that the CS sugar chain has inhibitory action against myoblast cell fusion. PMID:27775651

  3. Bacteriochlorophyllide c C-8(2) and C-12(1) methyltransferases are essential for adaptation to low light in Chlorobaculum tepidum.

    PubMed

    Gomez Maqueo Chew, Aline; Frigaard, Niels-Ulrik; Bryant, Donald A

    2007-09-01

    Bacteriochlorophyll (BChl) c is the major photosynthetic pigment in the green sulfur bacterium Chlorobaculum tepidum, in which it forms protein-independent aggregates that function in light harvesting. BChls c, d, and e are found only in chlorosome-producing bacteria and are unique among chlorophylls because of methylations that occur at the C-8(2) and C-12(1) carbons. Two genes required for these methylation reactions were identified and designated bchQ (CT1777) and bchR (CT1320). BchQ and BchR are members of the radical S-adenosylmethionine (SAM) protein superfamily; each has sequence motifs to ligate a [4Fe-4S] cluster, and we propose that they catalyze the methyl group transfers. bchQ, bchR, and bchQ bchR mutants of C. tepidum were constructed and characterized. The bchQ mutant produced BChl c that was not methylated at C-8(2), the bchR mutant produced BChl c that was not methylated at C-12(1), and the double mutant produced [8-ethyl, 12-methyl]-BChl c that lacked methylation at both the C-8(2) and C-12(1) positions. Compared to the wild type, the Qy absorption bands for BChl c in the mutant cells were narrower and blue shifted to various extents. All three mutants grew slower and had a lower cellular BChl c content than the wild type, an effect that was especially pronounced at low light intensities. These observations show that the C-8(2) and C-12(1) methylations of BChl c play important roles in the adaptation of C. tepidum to low light intensity. The data additionally suggest that these methylations also directly or indirectly affect the regulation of the BChl c biosynthetic pathway.

  4. Mismatched double-stranded RNA: polyI:polyC12U.

    PubMed

    2004-01-01

    Ampligen [polyI:polyC12U] is a mismatched double-stranded RNA that acts by inducing interferon production (immunomodulator) and by activating an intracellular enzyme (RNase-L) against viral RNA transcripts (antiviral). Ampligen, currently under development by Hemispherx Biopharma in the US, acts on the immunological system through T-lymphocyte stimulation and is indicated for the treatment of chronic fatigue syndrome and acquired immunodeficiency deficiency syndrome (AIDS), as part of the combined therapy. Ampligen is available for licensing worldwide. In February 2004, Fujisawa Deutschland GmbH, a subsidiary of Fujisawa Pharmaceutical Co., entered into an option agreement with Hemispherx Biopharma with the intent of becoming a distributor for Ampligen for the potential treatment of chronic fatigue syndrome in Germany, Switzerland and Austria. An option fee of 400,000 euros was paid pursuant to the terms of the option agreement and upon execution of the Distribution Agreement, Fujisawa will pay Hemispherx fees and milestone payments with a potential worth of several millions of dollars. In September 2003, Hemispherx Biopharma Inc. entered into an agreement with Guangdong Medicine Group Corporation to organise clinical trials, marketing, sales and distribution for both of its lead compounds, Ampligen and Alferon N in the People's Republic of China. The agreement stipulates that the Guangdong Medicine Group Corporation (GMC) will conduct clinical trials with Ampligen for the treatment of HIV. All costs related to the trials are to be covered by GMC. Additionally, GMC has to develop and implement marketing and promotional programmes. In May 2003, Hemispherx Biopharma and the Center for Cell and Gene Therapy entered into a research project agreement that will see Ampligen implemented in a protocol used in patients with relapsed EBV-positive Hodgkin's Lymphoma. In March 2002, Esteve and Hemispherx Biopharma entered into a collaborative agreement under which Esteve will

  5. 7 CFR 945.11 - Committee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN CERTAIN... Committee. Committee means the administrative committee, called the Idaho-Eastern Oregon Potato Committee...

  6. Publication committee meeting

    NASA Astrophysics Data System (ADS)

    The Publications Committee has oversight responsibility for the entire AGU publications program. It is supported in this activity by the Journals Board, the Books Board, and the Translations Board. The 1982-1984 committee is chaired by Thomas Graedel. Serving with him are David Atlas, Grant Gross, Jurate Landwehr, Peter Molnar, George Reid, and Rob Van der Voo.At its November 3-4 meeting the new committee spent much of its time acquainting its elf with the scope of the program, the current problems, and the potential opportunities. In addition to setting the background against which the work of the next two years would take place, the committee

  7. Committee Reports, May 2008

    NASA Astrophysics Data System (ADS)

    2008-08-01

    The Division's Executive Committee conducted several items of business at the New Orleans meeting. Elsewhere in this issue [see p 1032] is a listing of the candidates for Division offices for Fall 2008 election, approved by the Committee and later affirmed at the Division business meeting. Among items of specific interest to Division members is a plan to have the Journal of Chemical Education send an announcement to members when each issues goes online, and the Committee approved this use of the Division email list. It also approved plans presented by Amina El-Ashmawy and the BCCE committee to proceed with a bid from Pennsylvania State University for the 2012 BCCE.

  8. Consensus, contracts, and committees.

    PubMed

    Moreno, J D

    1991-08-01

    Following a brief account of the puzzle that ethics committees present for the Western Philosophical tradition, I will examine the possibility that social contract theory can contribute to a philosophical account of these committees. Passing through classical as well as contemporary theories, particularly Rawls' recent constructivist approach, I will argue that social contract theory places severe constraints on the authority that may legitimately be granted to ethics committees. This, I conclude, speaks more about the suitability of the theory to this level of analysis than about the ethics committee phenomenon itself.

  9. Investigation of Proton-Proton Short-Range Correlations via the C12(e,e'pp) Reaction

    NASA Astrophysics Data System (ADS)

    Shneor, R.; Monaghan, P.; Subedi, R.; Anderson, B. D.; Aniol, K.; Annand, J.; Arrington, J.; Benaoum, H.; Benmokhtar, F.; Bertin, P.; Bertozzi, W.; Boeglin, W.; Chen, J. P.; Choi, Seonho; Chudakov, E.; Cisbani, E.; Craver, B.; de Jager, C. W.; Feuerbach, R. J.; Frullani, S.; Garibaldi, F.; Gayou, O.; Gilad, S.; Gilman, R.; Glamazdin, O.; Gomez, J.; Hansen, J.-O.; Higinbotham, D. W.; Holmstrom, T.; Ibrahim, H.; Igarashi, R.; Jans, E.; Jiang, X.; Jiang, Y.; Kaufman, L.; Kelleher, A.; Kolarkar, A.; Kuchina, E.; Kumbartzki, G.; Lerose, J. J.; Lindgren, R.; Liyanage, N.; Margaziotis, D. J.; Markowitz, P.; Marrone, S.; Mazouz, M.; Meekins, D.; Michaels, R.; Moffit, B.; Nanda, S.; Perdrisat, C. F.; Piasetzky, E.; Potokar, M.; Punjabi, V.; Qiang, Y.; Reinhold, J.; Reitz, B.; Ron, G.; Rosner, G.; Saha, A.; Sawatzky, B.; Shahinyan, A.; Širca, S.; Slifer, K.; Solvignon, P.; Sulkosky, V.; Thompson, N.; Ulmer, P. E.; Urciuoli, G. M.; Voutier, E.; Wang, K.; Watson, J. W.; Weinstein, L. B.; Wojtsekhowski, B.; Wood, S.; Yao, H.; Zheng, X.; Zhu, L.

    2007-08-01

    We investigated simultaneously the C12(e,e'p) and C12(e,e'pp) reactions at Q2=2(GeV/c)2, xB=1.2, and in an (e, e'p) missing-momentum range from 300 to 600MeV/c. At these kinematics, with a missing momentum greater than the Fermi momentum of nucleons in a nucleus and far from the delta excitation, short-range nucleon-nucleon correlations are predicted to dominate the reaction. For (9.5±2)% of the C12(e,e'p) events, a recoiling partner proton was observed back-to-back to the C12(e,e'p) missing-momentum vector, an experimental signature of correlations.

  10. Experimental investigations of the sub-Coulomb 12C+12C and 12C+16O reactions

    NASA Astrophysics Data System (ADS)

    Courtin, S.; Fruet, G.; Jenkins, D. G.; Jiang, C. L.; Heine, M.; Montanari, D.; Santiago-Gonzalez, D.; Avila-Coronado, M.; Morris, L. G.; Goasduff, A.; Rehm, E.; Back, B.; Bourgin, D.; Beck, C.

    2017-06-01

    Cluster resonances in light heavy-ion systems like 12C+12C and 12C+16O may have a major impact on astrophysics stellar scenarios. Resonant radiative capture reactions have been studied for these systems at energies at and slightly below their Coulomb barriers to investigate the possible 12C-12C and 12C-16O molecular origin of the resonances. Spins have been attributed to the resonances and specificities of their γ-decay have been identified. At deep sub-barrier energies, a fusion cross section measurement using the particle-γ coincidence technique is discussed for the 12C+12C system. A new project is presented to possibly extend the 12C+12C S low-energy S factor study.

  11. 26 CFR 11.412(c)-12 - Extension of time to make contributions to satisfy requirements of section 412.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) TEMPORARY INCOME TAX REGULATIONS UNDER THE EMPLOYEE RETIREMENT INCOME SECURITY ACT OF 1974 § 11.412(c)-12 Extension of time to make...

  12. Direct fabrication of C12A7 electride target and room temperature deposition of thin films with low work function

    NASA Astrophysics Data System (ADS)

    Zou, Wenwei; Khan, Karim; Zhao, Xunna; Zhu, Chaoting; Huang, Jinhua; Li, Jia; Yang, Ye; Song, Weijie

    2017-03-01

    In this work, a high density (94%) and high electron concentration (2.3  ×  1020 cm‑3) doped C12A7 electride ceramic target for magnetron sputtering was prepared by directly heating the C12A7 nano powder to above its melting point. The effects of the temperature on the electron concentration was explored. Furthermore, a smooth (R q  =  0.6 nm), high transparent and low work function (Φ  =  2.9 eV) C12A7 electride thin film was deposited via magnetron sputtering. This result provides a suitable route for the fabrication of large-scale C12A7 electride film and has a bright prospect for application in optoelectronic devices.

  13. [Indoor air guide values for acetaldehyde. Announcement of the German Ad-hoc Working Group on Indoor Guidelines of the Indoor Air Hygiene Committee and of the States' Supreme Health Authorities].

    PubMed

    2013-10-01

    The German Ad-hoc Working Group on Indoor Guidelines of the Indoor Air Hygiene Committee and the States' Supreme Health Authorities is issuing indoor air guide values to protect public health. No suitable human studies are available for health evaluation of acetaldehyde in indoor air. In a well-documented subchronic inhalation animal study with rats assessed as reliable, local irritation effects were observed in nasal epithelia, most prominently in the olfactory epithelium with loss of olfactory neuronal cells. This study leads to a LOAEC of 48 mg acetaldehyde/m3 for continuous exposure for the endpoint nasal epithelium degeneration. By applying an interspecies factor of 1, a factor of 10 for interindividual variability, and a factor of 2 to account for the higher respiratory rate of children compared to adults, a health hazard guide value (RW II) of 1 mg acetaldehyde/m3 is obtained. A health precaution guide value (RW I) of 0.1 mg acetaldehyde/m3 is recommended.

  14. 77 FR 5801 - Consumer Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-06

    ... recommendations from its Universal Service Fund Working Group regarding Lifeline Modernization and the Connect to Compete Fund; a recommendation from its Broadband Working group regarding utilization of www.broadband.gov... Empowerment Working Group regarding the www.fcc.gov Web site. The Committee may also consider the matter...

  15. Summary of the Review of Committee Papers.

    ERIC Educational Resources Information Center

    Tittle, Carol K.

    Additional perspective on the program proposals developed by members of the Committee for National Program Priorities in Teacher Education was provided by a group of external reviewers representing community groups, school administrators and teachers, critics of teacher education, associations for professional groups in education, and university…

  16. Surface Adsorption of Oppositely Charged SDS:C(12)TAB Mixtures and the Relation to Foam Film Formation and Stability.

    PubMed

    Fauser, Heiko; Uhlig, Martin; Miller, Reinhard; von Klitzing, Regine

    2015-10-08

    The complexation, surface adsorption, and foam film stabiliztation of the oppositely charged surfactants, sodium dodecyl sulfate (SDS) and dodecyl trimethylammonium bromide (C12TAB), is analyzed. The SDS:C12TAB mixing ratio is systematically varied to investigate whether the adsorption of equimolar or irregular catanionic surfactant complexes, and thus a variation in surface charge (i.e., surface excess of either SDS or C12TAB), governs foam film properties. Surface tension measurements indicate that SDS and C12TAB interact electrostatically in order to form stoichometric catanionic surfactant complexes and enhance surface adsorption. On the other hand it can be demonstrated that the SDS:C12TAB mixing ratio and, thus, a change in surface charge and composition plays a decisive role in foam film stabilization. The present study demonstrates that varying the mixing ratio between SDS and C12TAB offers a tool for tailoring surface composition and foam film properties, which are therefore not exclusively mediated by the presence of equimolar catanionic surfactant complexes. The SDS:C12TAB net amount and mixing ratio determine the type, stability, and thinning behavior of the corresponding foam film. These observations indicate the formation of a mixed surface layer, composed of the catanionic surfactant species surrounded by either free SDS or C12TAB molecules in excess. Furthermore, a systematic variation in CBF-NBF transition kinetics is rationalized on the basis of a microscopic phase transition within the foam films. Fundamental knowlegde gained from this research gives insight into the surface adsorption and foam film formation of catanionic surfactant mixtures. The study helps researchers to understand basic mechanisms of foam film stabilization and to use resources more efficiently.

  17. 75 FR 64390 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... --Formal safety assessment --Piracy and armed robbery against ships --General cargo ship...

  18. 78 FR 29201 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-17

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... new measures Formal safety assessment Piracy and armed robbery against ships Implementation...

  19. 77 FR 57638 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-18

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... new measures; Formal safety assessment; Piracy and armed robbery against ships; Implementation...

  20. Using teams and committees effectively.

    PubMed

    Spilker, B

    1998-09-01

    In a corporate setting, the term "team" usually refers to members of a group with different responsibilities and/or skills working together to achieve a common goal or objective. The major reason why a company desires group as opposed to individual involvement is to derive sounder decisions. Two essential issues to resolve in establishing teams or committees are 1) who should be a member or representative; and 2) what is the charter or mandate for the group. Representatives join a team or group in numerous ways; four common methods are 1) appointment by the group member's supervisor; 2) recruitment by the team leader; 3) appointment by a senior manager; and 4) volunteering. There are various profiles of how groups can approach a decision, including "groupthink," the "ideal group process" and the "debating society" approach. Group meetings must be structured to ensure that decisions are reached and then implemented. Foresight and planning are essential prerequisites to have efficient teams and committees that work effectively and achieve their goals. (c) 1998 Prous Science. All rights reserved.

  1. Developmental Changes is Expression of Beta-Adrenergic Receptors in Cultures of C2C12 Skeletal Muscle Cells

    NASA Technical Reports Server (NTRS)

    Young, Ronald B.; Bridge, K. Y.; Vaughn, J. R.

    2000-01-01

    beta-Adrenergic receptor (bAR) agonists have been reported to modulate growth in several mammalian and avian species, and bAR agonists presumably exert their physiological action on skeletal muscle cells through this receptor. Because of the importance of bAR regulation on muscle protein metabolism in muscle cells, the objectives of this study were to determine the developmental expression pattern of the bAR population in C2C12 skeletal muscle cells, and to analyze changes in both the quantity and isoform expression of the major muscle protein, myosin. The number of bAR in mononucleated C2C12 cells was approximately 8,000 bAR per cell, which is comparable with the population reported in several other nonmuscle cell types. However, the bar population increased after myoblast fusion to greater than 50,000 bAR per muscle cell equivalent. The reasons for this apparent over-expression of bAR in C2C12 cells is not known. The quantity of myosin also increased after C2C12 myoblast fusion, but the quantity of myosin was less than that reported in primary muscle cell cultures. Finally, at least five different isoforms of myosin heavy chain could be resolved in C2C12 cells, and three of these exhibited either increased or decreased developmental regulation relative to the others. Thus, C2C12 myoblasts undergo developmental regulation of bAR population and myosin heavy chain isoform expression.

  2. Developmental Changes is Expression of Beta-Adrenergic Receptors in Cultures of C2C12 Skeletal Muscle Cells

    NASA Technical Reports Server (NTRS)

    Young, Ronald B.; Bridge, K. Y.; Vaughn, J. R.

    2000-01-01

    beta-Adrenergic receptor (bAR) agonists have been reported to modulate growth in several mammalian and avian species, and bAR agonists presumably exert their physiological action on skeletal muscle cells through this receptor. Because of the importance of bAR regulation on muscle protein metabolism in muscle cells, the objectives of this study were to determine the developmental expression pattern of the bAR population in C2C12 skeletal muscle cells, and to analyze changes in both the quantity and isoform expression of the major muscle protein, myosin. The number of bAR in mononucleated C2C12 cells was approximately 8,000 bAR per cell, which is comparable with the population reported in several other nonmuscle cell types. However, the bar population increased after myoblast fusion to greater than 50,000 bAR per muscle cell equivalent. The reasons for this apparent over-expression of bAR in C2C12 cells is not known. The quantity of myosin also increased after C2C12 myoblast fusion, but the quantity of myosin was less than that reported in primary muscle cell cultures. Finally, at least five different isoforms of myosin heavy chain could be resolved in C2C12 cells, and three of these exhibited either increased or decreased developmental regulation relative to the others. Thus, C2C12 myoblasts undergo developmental regulation of bAR population and myosin heavy chain isoform expression.

  3. Working with Groups.

    ERIC Educational Resources Information Center

    Morris, Joan, Ed.

    1984-01-01

    Describes nine Canadian programs for counseling groups of students. Topics include introducing computer-assisted guidance, future challenges for counselors, sociometry, sexuality, parent counseling, reluctant students, shyness, peer groups, education for living, and guidance advisory committees. (JAC)

  4. What Makes Groups Tick.

    ERIC Educational Resources Information Center

    Allcorn, Seth

    1985-01-01

    By reviewing this analysis of the behavior of both groups and individuals in groups, human resources managers can learn to tell whether committees, task forces, and departments may be encouraging or inhibiting the work they set out to do. (Author)

  5. Bioethics committees--a health communication approach.

    PubMed

    Fabregas, S M; Kreps, G L

    1999-03-01

    The modern health care system is being transformed as a consequence of scarce resources and better informed consumers. In this transformed health care system human communication has become a crucially important process. This essay examines the health communication functions performed by bioethics committees in health care delivery and makes recommendations for promoting the effective use of these groups within the modern health care system. The essay describes how bioethics committee can help establish a climate in which physicians and other committee members can share relevant health information, learn about patient and family concerns, promote health education and informed consent, and facilitate effective decision making about complex health care practice issues. It is argued that clear recognition and attention to the central role of effective communication within the bioethics committee is essential for the survival of the modern health care system since ethics committees depend on sharing relevant information to diagnose health problems, provide health care, promote health education, help in the formulation of new health care policies, and provide much-needed decision-making support to health care providers and consumers. The role that health communication expertise can perform in the effective operation and utilization of bioethics committees is described. We conclude that in order to provide needed support in addressing the many complexities of modern health care, bioethics committees must develop clear communication processes, norms, and roles.

  6. 11 CFR 116.2 - Debts owed by terminating committees, ongoing committees, and authorized committees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Debts owed by terminating committees, ongoing committees, and authorized committees. 116.2 Section 116.2 Federal Elections FEDERAL ELECTION COMMISSION GENERAL DEBTS OWED BY CANDIDATES AND POLITICAL COMMITTEES § 116.2 Debts owed by terminating committees...

  7. [Guide values for 1-butanol in indoor air. Report of the German Ad Hoc Working Group on Indoor Guidelines of the Indoor Air Hygiene Committee and of the States' Supreme Health Authorities].

    PubMed

    2014-06-01

    The German Ad Hoc Working Group on Indoor Guidelines of the Indoor Air Hygiene Committee and the States' Supreme Health Authorities is issuing indoor air guide values to protect public health. No human studies of sufficient quality are available for the evaluation of 1-butanol in indoor air. In a well-documented oral study on reproduction toxicity in rats, assessed as reliable, impairment of embryo development was observed. Benchmark modeling of the study data by US-EPA revealed a BMDL10 of 26.1 mg/kg b.w. per day. The working group used this BMDL10 as the point of departure for the derivation of the guide value II. Considering a human respiration rate of 20 m(3) per day and a human body weight of 70 kg, this dose was converted into an inhalative concentration. Applying a factor of 0.6 to account for the inhalative absorption rate, an allometric extrapolation factor from rat to human (factor 4), an interspecies factor of 2.5 for toxicodynamics, and a factor of 10 to account for individual differences (intraspecies factor), results in a health hazard guide value (RW II) of 2 mg 1-butanol/m(3). The benchmark dose calculation of the same study generated a BMDL05 of 12.4 mg/kg b.w. per day. Applying the same assessment factors as for RW II, a precautionary guide value (RW I) of 0.7 mg 1-butanol/m(3) indoor air is calculated.

  8. Animal Care Use Committees.

    ERIC Educational Resources Information Center

    Snyder, Margaret D.; And Others

    1992-01-01

    Describes the structure, activities, responsibilities, and practices of animal care and use committees established to review classroom activities and student research using animals. Provides six hypothetical situations with suggested solutions to test a committee's decision-making ability. Includes a proposed activity form for teachers. (MDH)

  9. Graphene oxide increases the viability of C2C12 myoblasts microencapsulated in alginate.

    PubMed

    Ciriza, J; Saenz del Burgo, L; Virumbrales-Muñoz, M; Ochoa, I; Fernandez, L J; Orive, G; Hernandez, R M; Pedraz, J L

    2015-09-30

    Cell microencapsulation represents a great promise for long-term drug delivery, but still several challenges need to be overcome before its translation into the clinic, such as the long term cell survival inside the capsules. On this regard, graphene oxide has shown to promote proliferation of different cell types either in two or three dimensions. Therefore, we planned to combine graphene oxide with the cell microencapsulation technology. We first studied the effect of this material on the stability of the capsules and next we analyzed the biocompatibility of this chemical compound with erythropoietin secreting C2C12 myoblasts within the microcapsule matrix. We produced 160 μm-diameter alginate microcapsules with increasing concentrations of graphene oxide and did not find modifications on the physicochemical parameters of traditional alginate microcapsules. Moreover, we observed that the viability of encapsulated cells within alginate microcapsules containing specific graphene oxide concentrations was enhanced. These results provide a relevant step for the future clinical application of graphene oxide on cell microencapsulation. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. Mitochondria dysfunction in lung cancer-induced muscle wasting in C2C12 myotubes.

    PubMed

    McLean, Julie B; Moylan, Jennifer S; Andrade, Francisco H

    2014-01-01

    Cancer cachexia is a syndrome which results in severe loss of muscle mass and marked fatigue. Conditioned media from cachexia-inducing cancer cells triggers metabolic dysfunction in skeletal muscle, including decreased mitochondrial respiration, which may contribute to fatigue. We hypothesized that Lewis lung carcinoma conditioned medium (LCM) would impair the mitochondrial electron transport chain (ETC) and increase production of reactive oxygen species, ultimately leading to decreased mitochondrial respiration. We incubated C2C12 myotubes with LCM for 30 min, 2, 4, 24 or 48 h. We measured protein content by western blot; oxidant production by 2',7'-dichlorofluorescin diacetate (DCF), 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF), and MitoSox; cytochrome c oxidase activity by oxidation of cytochrome c substrate; and oxygen consumption rate (OCR) of intact myotubes by Seahorse XF Analyzer. LCM treatment for 2 or 24 h decreased basal OCR and ATP-related OCR, but did not alter the content of mitochondrial complexes I, III, IV and V. LCM treatment caused a transient rise in reactive oxygen species (ROS). In particular, mitochondrial superoxide (MitoSOX) was elevated at 2 h. 4-Hydroxynonenal, a marker of oxidative stress, was elevated in both cytosolic and mitochondrial fractions of cell lysates after LCM treatment. These data show that lung cancer-conditioned media alters electron flow in the ETC and increases mitochondrial ROS production, both of which may ultimately impair aerobic metabolism and decrease muscle endurance.

  11. Caffeine and nicotine decrease acetylcholine receptor clustering in C2C12 myotube culture.

    PubMed

    Kordosky-Herrera, Kaia; Grow, Wade A

    2009-02-01

    As motor neurons approach skeletal muscle during development, agrin is released and induces acetylcholine receptor (AChR) clustering. Our laboratory investigates the effect of environmental agents on skeletal muscle development by using C2C12 cell culture. For the current project, we investigated both short-term and long-term exposure to caffeine, nicotine, or both, at physiologically relevant concentrations. Short-term exposure was limited to the last 48 h of myotube formation, whereas a long-term exposure of 2 weeks allowed for several generations of myoblast proliferation followed by myotube formation. Both agrin-induced and spontaneous AChR clustering frequencies were assessed. For agrin-induced AChR clustering, agrin was added for the last 16 h of myotube formation. Caffeine, nicotine, or both significantly decreased agrin-induced AChR clustering during short-term and long-term exposure. Furthermore, caffeine, nicotine, or both significantly decreased spontaneous AChR clustering during long-term, but not short-term exposure. Surprisingly, caffeine and nicotine in combination did not decrease AChR clustering beyond the effect of either treatment alone. We conclude that physiologically relevant concentrations of caffeine or nicotine decrease AChR clustering. Moreover, we predict that fetuses exposed to caffeine or nicotine may be less likely to form appropriate neuromuscular synapses.

  12. Rotenone induces reductive stress and triacylglycerol deposition in C2C12 cells.

    PubMed

    He, Quan; Wang, Miao; Petucci, Christopher; Gardell, Stephen J; Han, Xianlin

    2013-12-01

    Environmental rotenone is associated with Parkinson's disease due to its inhibitory property to the complex I of mitochondrial respiration chain. Although environmental pollution has been postulated as a causal factor for the increasing prevalence of obesity, the role of rotenone in the pathogenesis of obesity has not been studied. We employed muscle-derived cell C2C12 as a model and shotgun lipidomics as a tool for lipid analysis and found that treatment with rotenone led to the profound deposition of intracellular triacylglycerol (TAG) in a time- and dose-dependent fashion. The TAG deposition resulted from complex I inhibition. Further studies revealed that rotenone induced mitochondrial stress as shown by decreased mitochondrial oxygen consumption rate, increased NADH/NAD+ ratio (i.e., reductive stress) and mitochondrial metabolites. We demonstrated that rotenone activated fatty acid de novo synthesis and TAG synthesis and ultimately resulted in intracellular TAG deposition. These studies suggested that increased mitochondrial stresses might be an underlying mechanism responsible for TAG accumulation manifest in obesity. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. Carbon isotope ratio (13C/12C) of pine honey and detection of HFCS adulteration.

    PubMed

    Çinar, Serap B; Ekşi, Aziz; Coşkun, İlknur

    2014-08-15

    Carbon isotope ratio ((13)C/(12)C=δ(13)C) of 100 pine honey samples collected from 9 different localities by Mugla region (Turkey) in years 2006, 2007 and 2008 were investigated. The δ(13)Cprotein value of honey samples ranged between -23.7 and -26.6‰, while the δ(13)Choney value varied between -22.7 and -27‰. For 90% of the samples, the difference in the C isotope ratio of protein and honey fraction (δ(13)Cpro-δ(13)Chon) was -1.0‰ and/or higher. Therefore, it can be said that the generally anticipated minimum value of C isotope difference (-1.0‰) for honey is also valid for pine honey. On the other hand, C4 sugar value (%), which was calculated from the δ(13)Cpro-δ(13)Chon difference, was found to be linearly correlated with the amount of adulterant (HFCS) in pine honey. These results indicate that C4 sugar value is a powerful criteria for detecting HFCS adulteration in pine honey. The δ(13)Choney and δ(13)Cprotein-δ(13)Choney values of the samples did not show any significant differences in terms of both year and locality (P>0.05), while the δ(13)Cprotein values showed significant differences due to year (P<0.05) but not due to locality (P>0.05).

  14. Optogenetic approach for targeted activation of global calcium transients in differentiated C2C12 myotubes.

    PubMed

    Sebille, Stéphane; Ayad, Oualid; Chapotte-Baldacci, Charles-Albert; Cognard, Christian; Bois, Patrick; Chatelier, Aurélien

    2017-09-11

    Excitation-contraction coupling in muscle cells is initiated by a restricted membrane depolarization delimited within the neuromuscular junction. This targeted depolarization triggers an action potential that propagates and induces a global cellular calcium response and a consequent contraction. To date, numerous studies have investigated this excitation-calcium response coupling by using different techniques to depolarize muscle cells. However, none of these techniques mimic the temporal and spatial resolution of membrane depolarization observed in the neuromuscular junction. By using optogenetics in C2C12 muscle cells, we developed a technique to study the calcium response following membrane depolarization induced by photostimulations of membrane surface similar or narrower than the neuromuscular junction area. These stimulations coupled to confocal calcium imaging generate a global cellular calcium response that is the consequence of a membrane depolarization propagation. In this context, this technique provides an interesting, contactless and relatively easy way of investigation of calcium increase/release as well as calcium decrease/re-uptake triggered by a propagated membrane depolarization.

  15. An adaptable stage perfusion incubator for the controlled cultivation of C2C12 myoblasts.

    PubMed

    Kurth, Felix; Franco-Obregón, Alfredo; Bärtschi, Christoph A; Dittrich, Petra S

    2015-01-07

    Here we present a stage perfusion incubation system that allows for the cultivation of mammalian cells within PDMS microfluidic devices for long-term microscopic examination and analysis. The custom-built stage perfusion incubator is adaptable to any x-y microscope stage and is enabled for temperature, gas and humidity control as well as equipped with chip and tubing holder. The applied double-layered microfluidic chip allows the predetermined positioning and concentration of cells while the gas permeable PDMS material facilitates pH control via CO2 levels throughout the chip. We demonstrate the functionality of this system by culturing C2C12 murine myoblasts in buffer free medium within its confines for up to 26 hours. We moreover demonstrated the system's compatibility with various chip configurations, other cells lines (HEK-293 cells) and for longer-term culturing. The cost-efficient system are applicable for any type of PDMS-based cell culture system. Detailed technical drawings and specification to reproduce this perfusion incubation system is provided in the ESI.

  16. Assessment of the GECKO-A modeling tool using chamber observations for C12 alkanes

    NASA Astrophysics Data System (ADS)

    Aumont, B.; La, S.; Ouzebidour, F.; Valorso, R.; Mouchel-Vallon, C.; Camredon, M.; Lee-Taylor, J. M.; Hodzic, A.; Madronich, S.; Yee, L. D.; Loza, C. L.; Craven, J. S.; Zhang, X.; Seinfeld, J.

    2013-12-01

    Secondary Organic Aerosol (SOA) production and ageing is the result of atmospheric oxidation processes leading to the progressive formation of organic species with higher oxidation state and lower volatility. Explicit chemical mechanisms reflect our understanding of these multigenerational oxidation steps. Major uncertainties remain concerning the processes leading to SOA formation and the development, assessment and improvement of such explicit schemes is therefore a key issue. The development of explicit mechanism to describe the oxidation of long chain hydrocarbons is however a challenge. Indeed, explicit oxidation schemes involve a large number of reactions and secondary organic species, far exceeding the size of chemical schemes that can be written manually. The chemical mechanism generator GECKO-A (Generator for Explicit Chemistry and Kinetics of Organics in the Atmosphere) is a computer program designed to overcome this difficulty. GECKO-A generates gas phase oxidation schemes according to a prescribed protocol assigning reaction pathways and kinetics data on the basis of experimental data and structure-activity relationships. In this study, we examine the ability of the generated schemes to explain SOA formation observed in the Caltech Environmental Chambers from various C12 alkane isomers and under high NOx and low NOx conditions. First results show that the model overestimates both the SOA yields and the O/C ratios. Various sensitivity tests are performed to explore processes that might be responsible for these disagreements.

  17. Engineering Escherichia coli for production of C12–C14 polyhydroxyalkanoate from glucose

    PubMed Central

    Agnew, Daniel E.; Stevermer, Amanda K.; Youngquist, J. Tyler; Pfleger, Brian F.

    2013-01-01

    Demand for sustainable materials motivates the development of microorganisms capable of synthesizing products from renewable substrates. A challenge to commercial production of polyhydroxyalkanoates (PHA), microbially derived polyesters, is engineering metabolic pathways to produce a polymer with the desired monomer composition from an unrelated and renewable source. Here, we demonstrate a metabolic pathway for converting glucose into medium-chain-length (mcl)-PHA composed primarily of 3-hydroxydodecanoate monomers. This pathway combines fatty acid biosynthesis, an acyl-ACP thioesterase to generate desired C12 and C14 fatty acids, β-oxidation for conversion of fatty acids to (R)-3-hydroxyacyl-CoAs, and a PHA polymerase. A key finding is that Escherichia coli expresses multiple copies of enzymes involved in β-oxidation under aerobic conditions. To produce polyhydroxydodecanoate, an acyl-ACP thioesterase (BTE), an enoyl-CoA hydratase (phaJ3), and mcl-PHA polymerase (phaC2) were overexpressed in E. coli ΔfadRABIJ. Yields were improved through expression of an acyl-CoA synthetase resulting in production over 15% CDW – the highest reported production of mcl-PHA of a defined composition from an unrelated carbon source. PMID:23141473

  18. Antioxidant effects of whey protein on muscle C2C12 cells.

    PubMed

    Kerasioti, Efthalia; Stagos, Dimitrios; Priftis, Alexandros; Aivazidis, Stefanos; Tsatsakis, Aristidis M; Hayes, A Wallace; Kouretas, Demetrios

    2014-07-15

    In the present study, the in vitro scavenging activity of sheep whey protein against free radicals, as well as its reducing power were determined and compared with that of beef protein, soy protein and cow whey protein. Moreover, the possible protective effects of sheep whey protein from tert-butyl hydroperoxide (tBHP)-induced oxidative stress in muscle C2C12 cells were determined by assessing oxidative stress markers by flow cytometry and spectrophotometry. The results showed that sheep whey protein scavenged DPPH, ABTS(+) and OH radicals with IC50 values of 3.1, 4.1 and 1.8 mg of protein/ml. Moreover, the reducing power activity assessed with potassium ferricyanide of sheep whey protein was 1.3mg/ml. As regards to the antioxidant effects in muscle cell line, sheep whey protein at 0.78, 1.56, 3.12 and 6.24 mg of protein/ml increased GSH levels up to 138%, lowered TBARS levels up to 25% and decreased ROS levels up to 41.4%.

  19. Uncertainties of fluxes and 13C / 12C ratios of atmospheric reactive-gas emissions

    NASA Astrophysics Data System (ADS)

    Gromov, Sergey; Brenninkmeijer, Carl A. M.; Jöckel, Patrick

    2017-07-01

    We provide a comprehensive review of the proxy data on the 13C / 12C ratios and uncertainties of emissions of reactive carbonaceous compounds into the atmosphere, with a focus on CO sources. Based on an evaluated set-up of the EMAC model, we derive the isotope-resolved data set of its emission inventory for the 1997-2005 period. Additionally, we revisit the calculus required for the correct derivation of uncertainties associated with isotope ratios of emission fluxes. The resulting δ13C of overall surface CO emission in 2000 of -(25. 2 ± 0. 7) ‰ is in line with previous bottom-up estimates and is less uncertain by a factor of 2. In contrast to this, we find that uncertainties of the respective inverse modelling estimates may be substantially larger due to the correlated nature of their derivation. We reckon the δ13C values of surface emissions of higher hydrocarbons to be within -24 to -27 ‰ (uncertainty typically below ±1 ‰), with an exception of isoprene and methanol emissions being close to -30 and -60 ‰, respectively. The isotope signature of ethane surface emission coincides with earlier estimates, but integrates very different source inputs. δ13C values are reported relative to V-PDB.

  20. Thermal properties of light nuclei from 12C + 12C fusion-evaporation reactions

    NASA Astrophysics Data System (ADS)

    Morelli, L.; Baiocco, G.; D'Agostino, M.; Gulminelli, F.; Bruno, M.; Abbondanno, U.; Appannababu, S.; Barlini, S.; Bini, M.; Casini, G.; Cinausero, M.; Degerlier, M.; Fabris, D.; Gelli, N.; Gramegna, F.; Kravchuk, V. L.; Marchi, T.; Olmi, A.; Pasquali, G.; Piantelli, S.; Valdré, S.; Raduta, Ad R.

    2014-07-01

    The 12C + 12C reaction at 95 MeV has been studied through the complete charge identification of its products by means of the GARFIELD+RCo experimental set-up at INFN Laboratori Nazionali di Legnaro (LNL). In this paper, the first of a series of two, a comparison to a dedicated Hauser-Feshbach calculation allows selecting a set of dissipative events which corresponds, to a large extent, to the statistical evaporation of highly excited 24Mg. Information on the isotopic distribution of the evaporation residues in coincidence with their complete evaporation chain is also extracted. The set of data puts strong constraints on the behaviour of the level density (LD) of light nuclei above the threshold for particle emission. In particular, a fast increase of the LD parameter with excitation energy is supported by the data. Residual deviations from a statistical behaviour are seen in two specific channels, and tentatively associated with a contamination from direct reactions and/or α-clustering effects. These channels are studied in further details in the second paper of the series.

  1. Electrical pulse stimulation decreases electrochemical Na(+) and K(+) gradients in C2C12 myotubes.

    PubMed

    Danilov, Kirill; Sidorenko, Svetlana; Milovanova, Kseniya; Klimanova, Elizaveta; Kapilevich, Leonid V; Orlov, Sergei N

    2017-11-18

    Electrical pulse stimulation (EPS)-treated cultured myotubes are widely employed as an in vitro model of muscle contraction. Here we examined time-dependent EPS action and dose-dependent ouabain action on [Na(+)]i and [K(+)]i in C2C12 myotubes. After 2 h of EPS (40 V, 1 Hz, 10 ms) [Na(+)]i increased by ∼150% whereas [K(+)]i declined by ∼20%. 3 μM ouabain had a negligible impact on [Na(+)]i and [K(+)]i in control cells but increased the [Na(+)]i/[K(+)]i ratio in EPS-treated myotubes by 85%. Thus, our results show for the first time that EPS results in dissipation of Na(+) and K(+) gradients in cultured myotubes and suggest that the augmented production of endogenous cardiotonic steroids may contribute to elevation of the [Na(+)]i/[K(+)]i ratio in exercising muscle. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. 22 CFR 214.2 - Definition of advisory committee.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Definition of advisory committee. 214.2 Section 214.2 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT General..., conference, panel, task force, or other similar group, or any subcommittee or sub-group thereof, which...

  3. 76 FR 82031 - Commercial Space Transportation Advisory Committee; Public Teleconference

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ... Advisory Committee Risk Management Working Group Teleconference. SUMMARY: Pursuant to Section 10(a)(2) of... teleconference of the Commercial Space Transportation Advisory Committee (COMSTAC) Risk Management Working Group. The teleconference will take place on Tuesday, January 24, 2012, starting at 1:30 p.m....

  4. Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (HMSD1111, LB4033_H)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (HMSD1111, LB4033_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  5. Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (HMSD1111, LB4039_H)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (HMSD1111, LB4039_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  6. Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (VMSD1211, LB3967_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (VMSD1211, LB3967_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  7. Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (VMSD1211, LB3961_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (VMSD1211, LB3961_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  8. G3-C12 Peptide Reverses Galectin-3 from Foe to Friend for Active Targeting Cancer Treatment.

    PubMed

    Sun, Wei; Li, Lian; Yang, Qingqing; Shan, Wei; Zhang, Zhirong; Huang, Yuan

    2015-11-02

    Galectin-3 is overexpressed by numerous carcinomas and is a potential target for active tumor treatments. On the other hand, galectin-3 also plays a key role in cancer progression and prevents cells from undergoing apoptosis, thereby offsetting the benefits of active targeting drugs. However, the relative contribution of the protective antiapoptotic effects of galectin-3 and the proapoptotic effects of galectin-3-targeted therapies has remained yet unrevealed. Here, we show that a galectin-3-binding peptide G3-C12 could reverse galectin-3 from foe to friend for active targeting delivery system. Results showed G3-C12 modified N-(2-hydroxypropyl)methacrylamide copolymer doxorubicin conjugates (G3-C12-HPMA-Dox) could internalize into galectin-3 overexpressed PC-3 cells via a highly specific ligand-receptor pathway (2.2 times higher cellular internalization than HPMA-Dox). The internalized Dox stimulated the translocation of galectin-3 to the mitochondria to prevent from apoptosis. In turn, this caused G3-C12-HPMA-Dox to concentrate into the mitochondria after binding to galectin-3 intracellularly. Initially, mitochondrial galectin-3 weakened Dox-induced mitochondrial damage; however, as time progressed, G3-C12 active-mediation allowed increasing amounts of Dox to be delivered to the mitochondria, which eventually induced higher level of apoptosis than nontargeted copolymers. In addition, G3-C12 downregulates galectin-3 expression, 0.43 times lower than control cells, which could possibly be responsible for the suppressed cell migration. Thus, G3-C12 peptide exerts sequential targeting to both cell membrane and mitochondria via regulating galectin-3, and eventually reverses and overcomes the protective effects of galectin-3; therefore, it could be a promising agent for the treatment of galectin-3-overexpressing cancers.

  9. Cortisol effect on heat shock proteins in the C2C12 and 3T3-L1 cells.

    PubMed

    Ravikumar, Sambandam; Muthuraman, Pandurangan

    2014-08-01

    The present study was carried out to understand the effect of cortisol on heat shock protein system (Hsps) in the C2C12 and 3T3-L1 cells under co-culture system. Cells were co-cultured by using Transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3T3-L1 cells. Each cell type was grown independently on the Transwell plates. After cell differentiation, inserts containing 3T3-L1 cells were transferred to C2C12 plates and inserts containing C2C12 cells transferred to 3T3-L1 plates. Ten micrograms per microliter of cortisol was added to the medium. Following 72 h of treatment, the cells in the lower wells were harvested for analysis. Heat shock proteins (Hsps) such as Hsp27, Hsp70, and Hsp90 were selected for the analysis. The qRT-PCR results showed the significant increase in the mRNA expression of as Hsp27, Hsp70, and Hsp90. In addition, confocal microscopical investigation showed the cortisol treatment increases Hsps expressions in the mono and co-cultured C2C12 and 3T3-L1 cells. From the results, we concluded that the cortisol increases Hsps expression in the co-cultured C2C12 and 3T3-L1 cells, which is differed from one-dimensional mono-cultured C2C12 and 3T3-L1 cells.

  10. Control of HIV-1 by an HLA-B*52:01-C*12:02 protective haplotype.

    PubMed

    Chikata, Takayuki; Murakoshi, Hayato; Koyanagi, Madoka; Honda, Kazutaka; Gatanaga, Hiroyuki; Oka, Shinichi; Takiguchi, Masafumi

    2017-09-14

    HLA-B*52:01-C*12:02, which is found in approximately 20 % of all Japanese, is well known to be associated with ulcerative colitis and Takayasu arteritis. This haplotype is also known to be a protective one in HIV-1-infective individuals. Recent studies showed that HLA-B*52:01-restricted HIV-1-specific T cells suppress HIV-1 and that HLA-C*12:02 together with KIR2DL2 play an important role in NK cell-mediated control of HIV-1. However, the role of HLA-C*12:02-restricted CTLs in suppression of HIV-1 replication remains unknown. In the present study, we demonstrated that HLA-C*12:02-restricted CTLs specific for 2 immunodominant epitopes, Pol IY11 and Nef MY9, contributed to the suppression of HIV-1 replication in HIV-1-infected individuals. Further analysis demonstrated that these 2 HLA-C*12:02-restricted CTLs together with 4 HLA-B*52:01-restricted ones effectively suppressed HIV-1 in individuals having the HLA-B*52:01-C*12:02 haplotype. Thus, both HLA-C*12:02 and HLA-B*52:01 alleles contribute to HIV-1 suppression via both HIV-1-specific CTLs and NK cells in individuals having this haplotype. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

  11. Pesticide Program Dialogue Committee (PPDC)

    EPA Pesticide Factsheets

    The Pesticide Program Dialogue Committee, a permanent, broadly representative advisory committee, meets with EPA on a regular basis to discuss pesticide regulatory, policy, and program implementation issues.

  12. The Executive Committee. Effective Committees. Board Basics.

    ERIC Educational Resources Information Center

    Ingram, Richard T.

    1996-01-01

    The executive board of the college or university governing board has the central purpose of strengthening the board's performance by helping it function efficiently and effectively. Because the executive committee can undermine trustee morale by abusing its authority, the entire governing board must decide the extent of the powers delegated to the…

  13. The Audit Committee. Effective Committees. Board Basics.

    ERIC Educational Resources Information Center

    Ostrom, John S.

    1996-01-01

    In many colleges and universities the responsibility of the governing board's audit committee is to arrange and oversee regular audits of financial activities, comply with donor restrictions on gifts, adhere to laws and regulations, and conform to established board policies. Membership of three to five is usually sufficient, and while the…

  14. The Compensation Committee. Effective Committees. Board Basics.

    ERIC Educational Resources Information Center

    Tranquada, Robert E.

    2001-01-01

    This booklet describes some of the practices of committees charged with setting the compensation of the college or university president or chancellor. Whether the institution is private or public, the president's income will become public information, and apart from any public relations implications, it simply makes good sense for the compensation…

  15. The Compensation Committee. Effective Committees. Board Basics.

    ERIC Educational Resources Information Center

    Tranquada, Robert E.

    2001-01-01

    This booklet describes some of the practices of committees charged with setting the compensation of the college or university president or chancellor. Whether the institution is private or public, the president's income will become public information, and apart from any public relations implications, it simply makes good sense for the compensation…

  16. Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area.

    PubMed

    Han, Xue-Mei; Liu, Yu-Rong; Zheng, Yuan-Ming; Zhang, Xiao-Xia; He, Ji-Zheng

    2014-01-01

    Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is threatening human health and environmental safety. Investigating the relative prevalence of different PAH-degrading genes in PAH-polluted soils and searching for potential bioindicators reflecting the impact of PAH pollution on microbial communities are useful for microbial monitoring, risk evaluation, and potential bioremediation of soils polluted by PAHs. In this study, three functional genes, pdo1, nah, and C12O, which might be involved in the degradation of PAHs from a coke factory, were investigated by real-time quantitative PCR (qPCR) and clone library approaches. The results showed that the pdo1 and C12O genes were more abundant than the nah gene in the soils. There was a significantly positive relationship between the nah or pdo1 gene abundances and PAH content, while there was no correlation between C12O gene abundance and PAH content. Analyses of clone libraries showed that all the pdo1 sequences were grouped into Mycobacterium, while all the nah sequences were classified into three groups: Pseudomonas, Comamonas, and Polaromonas. These results indicated that the abundances of nah and pdo1 genes were positively influenced by levels of PAHs in soil and could be potential microbial indicators reflecting the impact of soil PAH pollution and that Mycobacteria were one of the most prevalent PAHs degraders in these PAH-polluted soils. Principal component analysis (PCA) and correlation analyses between microbial parameters and environmental factors revealed that total carbon (TC), total nitrogen (TN), and dissolved organic carbon (DOC) had positive effects on the abundances of all PAH-degrading genes. It suggests that increasing TC, TN, and DOC inputs could be a useful way to remediate PAH-polluted soils.

  17. Acute toxicity of the cationic surfactant C12-benzalkonium in different bioassays: how test design affects bioavailability and effect concentrations.

    PubMed

    Chen, Yi; Geurts, Marc; Sjollema, Sascha B; Kramer, Nynke I; Hermens, Joop L M; Droge, Steven T J

    2014-03-01

    Using an ion-exchange-based solid-phase microextraction (SPME) method, the freely dissolved concentrations of C12-benzalkonium were measured in different toxicity assays, including 1) immobilization of Daphnia magna in the presence or absence of dissolved humic acid; 2) mortality of Lumbriculus variegatus in the presence or absence of a suspension of Organisation for Economic Co-Operation and Development (OECD) sediment; 3) photosystem II inhibition of green algae Chlorella vulgaris; and 4) viability of in vitro rainbow trout gill cell line (RTgill-W1) in the presence or absence of serum proteins. Furthermore, the loss from chemical adsorption to the different test vessels used in these tests was also determined. The C12-benzalkonium sorption isotherms to the different sorbent phases were established as well. Our results show that the freely dissolved concentration is a better indicator of the actual exposure concentration than the nominal or total concentration in most test assays. Daphnia was the most sensitive species to C12-benzalkonium. The acute Daphnia and Lumbriculus tests both showed no enhanced toxicity from possible ingestion of sorbed C12-benzalkonium in comparison with water-only exposure, which is in accordance with the equilibrium partitioning theory. Moreover, the present study demonstrates that commonly used sorbent phases can strongly affect bioavailability and observed effect concentrations for C12-benzalkonium. Even stronger effects of decreased actual exposure concentrations resulting from sorption to test vessels, cells, and sorbent phases can be expected for more hydrophobic cationic surfactants.

  18. TEAD1 controls C2C12 cell proliferation and differentiation and regulates three novel target genes.

    PubMed

    Wang, Fengli; Wang, Hongyang; Wu, Hao; Qiu, Haifang; Zeng, Cuiping; Sun, Ling; Liu, Bang

    2013-03-01

    TEAD1 is a transcription factor involved in activation of muscle specific genes, such as the cardiac muscle troponin T gene, skeletal muscle actin, myosin heavy chains genes. Here, we reported that TEAD1 was expressed ubiquitously in different mouse tissues and was up-regulated in differentiation process of the mouse myoblast cell line C2C12. Functional assay revealed that overexpression of TEAD1 gene can arrest the C2C12 cell cycle and promote C2C12 cell differentiation. To understand the physiological role of TEAD1 in muscle development, three new regulated genes of TEAD1, Mrpl21, Ndufa6 and Ccne1, were identified by expression analysis, promoter activity measurement assay. The expression patterns of target genes were detected in the cell differentiation process. The Mrpl21 and Ndufa6 genes were up-regulated in cell differentiation while Ccne1 gene was significantly down-regulated. Overexpression of Mrpl21 and Ndufa6 in C2C12 can up-regulate Myh4 gene expression thus promote C2C12 differentiation, but did not affect cell cycle. Co-overexpression of Ccne1 with Ndufa6 resulted in Myh4 expression decrease and the number of S-phase cells slight increase. Together, our results suggested that TEAD1 may mediate muscle development through its target genes, Mrpl21, Ndufa6 and Ccne1. Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.

  19. Screening of natural medicines that efficiently activate neurite outgrowth in PC12 cells in C2C12-cultured medium.

    PubMed

    Uezato, Tadayoshi; Sato, Eiji; Miura, Naoyuki

    2012-02-01

    We have studied the effects of natural medicines on neurite outgrowth in PC12D cells in a cultured medium of C2C12 cells. Derived from mouse myoblasts, the C2C12 cells secrete neurotrophic factors including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3). The secretion of these neurotrophins from C2C12 cells stimulate neurite outgrowth in PC12D cells. We have screened a total of 120 samples and found five natural medicines: Trichosanthes Root, Asiasarum Root, Lycium Bark, Sinomenium Stem, and Dictamni radicis Cortex, that enhance the activity of C2C12-cultured medium to stimulate neurite outgrowth in PC12D cells. These natural medicines promoted not only neurite outgrowth but also stabilized the neurite formation in PC12D cells for several days. RT-PCR analysis showed that NGF was significantly increased with Trichosanthes and Lycium Bark. However, BDNF was slightly decreased with Lycium Bark, Sinomenium Stem, and Dictamni radicis Cortex. NT-3 was increased slightly by all of these natural medicines except Sinomenium Stem. All these five natural medicines significantly increased the number and length of neurites in PC12D cells in co-culture with C2C12 cells.

  20. Truncated human LMP-1 triggers differentiation of C2C12 cells to an osteoblastic phenotype in vitro.

    PubMed

    Fei, Qinming; Boden, Scott D; Sangadala, Sreedhara; Viggeswarapu, Manjula; Liu, Yunshan; Titus, Louisa

    2007-09-01

    LIM mineralization protein-1 (LMP-1) is a novel intracellular osteoinductive protein that has been shown to induce bone formation both in vitro and in vivo. LMP-1 contains an N-terminal PDZ domain and three C-terminal LIM domains. In this study, we investigated whether a truncated form of human LMP-1 (hLMP-1[t]), lacking the three C-terminal LIM domains, triggers the differentiation of pluripotent myoblastic C(2)C(12) cells to the osteoblast lineage. C(2)C(12) cells were transiently transduced with Ad5-hLMP-1(t)-green fluorescent protein or viral vector control. The expression of hLMP-1(t) RNA and the truncated protein were examined. The results showed that hLMP-1(t) blocked myotube formation in C(2)C(12) cultures and significantly enhanced the alkaline phosphatase (ALP) activity. In addition, the expressions of ALP, osteocalcin, and bone morphogenetic protein (BMP)-2 and BMP-7 genes were also increased. The induction of these key osteogenic markers suggests that hLMP-1(t) can trigger the pluripotent myoblastic C2C12 cells to differentiate into osteoblastic lineage, thus extending our previous observation that LMP-1 and LMP-1(t) enhances the osteoblastic phenotype in cultures of cells already committed to the osteoblastic lineage. Therefore, C(2)C(12) cells are an appropriate model system for the examination of LMP-1 induction of the osteoblastic phenotype and the study of mechanisms of LMP-1 action.

  1. Introduction and Committees

    NASA Astrophysics Data System (ADS)

    Angelova, Maia; Zakrzewski, Wojciech; Hussin, Véronique; Piette, Bernard

    2011-03-01

    This volume contains contributions to the XXVIIIth International Colloquium on Group-Theoretical Methods in Physics, the GROUP 28 conference, which took place in Newcastle upon Tyne from 26-30 July 2010. All plenary and contributed papers have undergone an independent review; as a result of this review and the decisions of the Editorial Board most but not all of the contributions were accepted. The volume is organised as follows: it starts with notes in memory of Marcos Moshinsky, followed by contributions related to the Wigner Medal and Hermann Weyl prize. Then the invited talks at the plenary sessions and the public lecture are published followed by contributions in the parallel and poster sessions in alphabetical order. The Editors:Maia Angelova, Wojciech Zakrzewski, Véronique Hussin and Bernard Piette International Advisory Committee Michael BaakeUniversity of Bielefeld, Germany Gerald DunneUniversity of Connecticut, USA J F (Frank) GomesUNESP, Sao Paolo, Brazil Peter HanggiUniversity of Augsburg, Germany Jeffrey C LagariasUniversity of Michigan, USA Michael MackeyMcGill University, Canada Nicholas MantonCambridge University, UK Alexei MorozovITEP, Moscow, Russia Valery RubakovINR, Moscow, Russia Barry SandersUniversity of Calgary, Canada Allan SolomonOpen University, Milton Keynes, UK Christoph SchweigertUniversity of Hamburg, Germany Standing Committee Twareque AliConcordia University, Canada Luis BoyaSalamanca University, Spain Enrico CeleghiniFirenze University, Italy Vladimir DobrevBulgarian Academy of Sciences, Bulgaria Heinz-Dietrich DoebnerHonorary Member, Clausthal University, Germany Jean-Pierre GazeauChairman, Paris Diderot University, France Mo-Lin GeNankai University. China Gerald GoldinRutgers University, USA Francesco IachelloYale University, USA Joris Van der JeugtGhent University, Belgium Richard KernerPierre et Marie Curie University, France Piotr KielanowskiCINVESTAV, Mexico Alan KosteleckyIndiana University, USA Mariano del Olmo

  2. Attenuation of muscle wasting in murine C2C 12 myotubes by epigallocatechin-3-gallate.

    PubMed

    Mirza, Kamran A; Pereira, Suzette L; Edens, Neile K; Tisdale, Michael J

    2014-12-01

    Loss of muscle protein is a common feature of wasting diseases where currently treatment is limited. This study investigates the potential of epigallocatechin-3-gallate (EGCg), the most abundant catechin in green tea, to reverse the increased protein degradation and rescue the decreased protein synthesis which leads to muscle atrophy. Studies were conducted in vitro using murine C2C12 myotubes. Increased protein degradation and reduced rates of protein synthesis were induced by serum starvation and tumour necrosis factor-α (TNF-α). EGCg effectively attenuated the depression of protein synthesis and increase in protein degradation in murine myotubes at concentrations as low as 10 μM. Serum starvation increased expression of the proteasome 20S and 19S subunits, as well as the proteasome 'chymotrypsin-like' enzyme activity, and these were all attenuated down to basal values in the presence of EGCg. Serum starvation did not increase expression of the ubiquitin ligases MuRF1 and MAFbx, but EGCg reduced their expression below basal levels, possibly due to an increased expression of phospho Akt (pAkt) and phospho forkhead box O3a (pFoxO3a). Attenuation of protein degradation by EGCg was increased in the presence of ZnSO4, suggesting an EGCg-Zn(2+) complex may be the active species. The ability of EGCg to attenuate depressed protein synthesis and increase protein degradation in the myotubule model system suggests that it may be effective in preserving skeletal muscle mass in catabolic conditions.

  3. Line Positions and Intensities for the ν12 Band of 13C12CH_6

    NASA Astrophysics Data System (ADS)

    Devi, V. Malathy; Benner, D. Chris; Sung, Keeyoon; Crawford, Timothy J.; Mantz, Arlan; Smith, Mary Ann H.

    2014-06-01

    High-resolution, high signal-to-noise spectra of mono-substituted 13C-ethane (13C12CH_6) in the 12.2 μm region were recorded with a Bruker IFS 125HR Fourier transform spectrometer. The spectra were obtained for four sample pressures at three different temperatures between 130 and 208 K using a 99% 13C-enriched ethane sample contained in a 20.38-cm long coolable absorption cell. A multispectrum nonlinear least squares fitting technique was used to fit the same intervals in the four spectra simultaneously to determine line positions and intensities. Similar to our previous analyses of 12C_2H_6 spectra in this same region, constraints were applied to accurately fit each pair of doublet components arising from torsional Coriolis interaction of the excited ν12 = 1 state with the nearby torsional ν_6 = 3 state. Line intensities corresponding to each spectrum temperature (130 K, 178 K and 208 K) are reported for 1660 ν12 absorption lines for which the assignments are known, and integrated intensities are estimated as the summation of the measured values. The measured line positions and intensities (re-scaled to 296 K) are compared with values in recent editions of spectroscopic databases. K. Sung, A. W. Mantz, L. R. Brown, et al., J. Mol. Spectrosc., 162 (2010) 124-134. D. C. Benner, C. P. Rinsland, V. Malathy Devi, M. A. H. Smith and D. Atkins, JQSRT, 53 (1995) 705-721. V. Malathy Devi, C. P. Rinsland, D. Chris Benner, et al., JQSRT, 111 (2010) 1234-1251 V. Malathy Devi, D. Chris Benner, C. P. Rinsland, et al., JQSRT, 111 (2010) 2481-2504. Research described in this paper was performed at Connecticut College, the College of William and Mary, NASA Langley Research Center and the Jet Propulsion Laboratory, California Institute of Technology, under contracts and cooperative agreements with the National Aeronautics and Space Administration.

  4. Regulation of nonmuscle myosin II during 3-methylcholanthrene induced dedifferentiation of C2C12 myotubes

    SciTech Connect

    Dey, Sumit K.; Saha, Shekhar; Das, Provas; Das, Mahua R.; Jana, Siddhartha S.

    2014-08-01

    3-Methylcholanthrene (3MC) induces tumor formation at the site of injection in the hind leg of mice within 110 days. Recent reports reveal that the transformation of normal muscle cells to atypical cells is one of the causes for tumor formation, however the molecular mechanism behind this process is not well understood. Here, we show in an in vitro study that 3MC induces fragmentation of multinucleate myotubes into viable mononucleates. These mononucleates form colonies when they are seeded into soft agar, indicative of cellular transformation. Immunoblot analysis reveals that phosphorylation of myosin regulatory light chain (RLC{sub 20}) is 5.6±0.5 fold reduced in 3MC treated myotubes in comparison to vehicle treated myotubes during the fragmentation of myotubes. In contrast, levels of myogenic factors such as MyoD, Myogenin and cell cycle regulators such as Cyclin D, Cyclin E1 remain unchanged as assessed by real-time PCR array and reverse transcriptase PCR analysis, respectively. Interestingly, addition of the myosin light chain kinase inhibitor, ML-7, enhances the fragmentation, whereas phosphatase inhibitor perturbs the 3MC induced fragmentation of myotubes. These results suggest that decrease in RLC{sub 20} phosphorylation may be associated with the fragmentation step of dedifferentiation. - Highlights: • 3-Methylcholanthrene induces fragmentation of C2C12-myotubes. • Dedifferentiation can be divided into two steps – fragmentation and proliferation. • Fragmentation is associated with rearrangement of nonmuscle myosin II. • Genes associated with differentiation and proliferation are not altered during fragmentation. • Phosphorylation of myosin regulatory light chain is reduced during fragmentation.

  5. 13C/12C isotope ratio MS analysis of testosterone, in chemicals and pharmaceutical preparations.

    PubMed

    de la Torre, X; González, J C; Pichini, S; Pascual, J A; Segura, J

    2001-02-01

    The 13C/12C ratio can be used to detect testosterone misuse in sport because (semi)-synthetic testosterone is supposed to have a 13C abundance different from that of endogenous natural human testosterone. In this study, gas chromatography/combustion isotope ratio mass spectrometry (GC/C/IRMS) analysis for the measurement of the delta 13C/1000 value of testosterone from esterified forms of 13 pharmaceutical preparations, six reagent grade chemicals and three bulk materials (raw materials used in pharmaceutical proarations) obtained world-wide was investigated after applying a strong acidic solvolytic procedure. Mean delta 13C/1000 values of non esterified (free) testosterone from chemicals and bulk materials of several testosterone esters were in the range: -25.91/-32.82/1000 while the value obtained for a (semi)-synthetic, reagent grade, free testosterone was -27.36/1000. The delta 13C/1000 results obtained for testosterone from the pharmaceuticals investigated containing testosterone esters were quite homogeneous (mean and S.D. of delta 13C/1000 values of free testosterone: 27.43 +/- 0.76/1000), being the range between -26.18 and -30.04/1000. Values described above were clearly different from those reported by several authors for endogenous natural human testosterone and its main metabolites excreted into the urine in non-consumers of testosterone (delta 13C/1000 range: from -21.3 to -24.4/1000), while they were similar to those of urinary testosterone and metabolites from individuals treated with testosterone esters and testosterone precursors. This finding justifies the fact that administration of these pharmaceutical formulations led to a statistical decrease of carbon isotope ratio of urinary testosterone and its main metabolites in treated subjects.

  6. Phase behavior of 1-dodecyl-3-methylimidazolium fluorohydrogenate salts (C12MIm(FH)(n)F, n = 1.0-2.3) and their anisotropic ionic conductivity as ionic liquid crystal electrolytes.

    PubMed

    Xu, Fei; Matsumoto, Kazuhiko; Hagiwara, Rika

    2012-08-23

    The effects of the HF composition, n, in 1-dodecyl-3-methylimidazolium fluorohydrogenate salts (C(12)MIm(FH)(n)F, n = 1.0-2.3) on their physicochemical and structural properties have been investigated using infrared spectroscopy, thermal analysis, polarized optical microscopy, X-ray diffraction, and anisotropic ionic conductivity measurements. The phase diagram of C(12)MIm(FH)(n)F (n vs transition temperature) suggests that C(12)MIm(FH)(n)F is a mixed crystal system that has a boundary around n = 1.9. For all compositions, a liquid crystalline mesophase with a smectic A interdigitated bilayer structure is observed. The temperature range of the mesophase decreases with increasing n value (from 61.8 °C for C(12)MIm(FH)(1.0)F to 37.0 °C for C(12)MIm(FH)(2.3)F). The layer spacing of the smectic structure decreases with increasing n value or increasing temperature. Two structural types with different layer spacings are observed in the crystalline phase (type I, 1.0 ≤ n ≤ 1.9, and type II, 1.9 ≤ n ≤ 2.3). Ionic conductivities parallel and perpendicular to the smectic layers (σ(||) and σ([perpendicular])) increase with increasing n value, whereas the anisotropy of the ionic conductivities (σ(||)/σ([perpendicular])) is independent of the n value, since the thickness of the insulating sheet formed by the dodecyl group remains nearly unchanged.

  7. 78 FR 40144 - Governmentwide Travel Advisory Committee (GTAC); Upcoming Public Advisory Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-03

    ... ADMINISTRATION Governmentwide Travel Advisory Committee (GTAC); Upcoming Public Advisory Committee Meeting AGENCY... Governmentwide Travel Advisory Committee (GTAC) (the Committee), is a Federal Advisory Committee established in..., Designated Federal Officer (DFO), Governmentwide Travel Advisory Committee (GTAC), Office of...

  8. 75 FR 30002 - Federal Advisory Committee; Threat Reduction Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ... Office of the Secretary Federal Advisory Committee; Threat Reduction Advisory Committee AGENCY... the charter for the Threat Reduction Advisory Committee (hereafter referred to as the Committee). FOR... Acquisition, Technology and Logistics and the Director of the Defense Threat Reduction Agency on the following...

  9. 76 FR 36613 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an.../imo . Dated: June 14, 2011. Greg O'Brien, Shipping Coordinating Committee, Department of State...

  10. 77 FR 22057 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... Secretary, Shipping Coordinating Committee, Department of State. BILLING CODE 4710-09-P ...

  11. 75 FR 75532 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an.../imo . Dated: November 24, 2010. Jon Trent Warner, Executive Secretary, Shipping Coordinating Committee...

  12. 77 FR 52106 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-28

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an..., Executive Secretary, Shipping Coordinating Committee, Department of State. BILLING CODE 4710-09-P ...

  13. 78 FR 48217 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-07

    ... Doc No: 2013-19083] DEPARTMENT OF STATE [Public Notice 8413] Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an open meeting at 9:30 a... Secretary, Shipping Coordinating Committee, Department of State. [FR Doc. 2013-19083 Filed 8-6-13; 8:45 am...

  14. 75 FR 10546 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... . ] Dated: March 2, 2010. Jon Trent Warner, Executive Secretary, Shipping Coordinating Committee, Department...

  15. 78 FR 32699 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-31

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... Secretary, Shipping Coordinating Committee, Department of State. BILLING CODE 4710-09-P ...

  16. 75 FR 9616 - Committee Management Renewals

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-03

    ... Committee Management Renewals The NSF management officials having responsibility for three advisory... Committee Management Secretariat, General Services Administration. Committees: Advisory Committee for...-7488. Susanne Bolton, Committee Management Officer. BILLING CODE 7555-01-P ...

  17. 77 FR 12086 - Committee Management Renewals

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-28

    ... Committee Management Renewals The NSF management officials having responsibility for three advisory... Committee Management Secretariat, General Services Administration. Committees: Advisory Committee for.... Dated: February 22, 2012. Susanne Bolton, Committee Management Officer. BILLING CODE 7555-01-P ...

  18. 75 FR 36698 - Committee Management Renewals

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-28

    ... Committee Management Renewals The NSF management officials having responsibility for the advisory committees... Management Secretariat, General Services Administration. Committees Committee on Equal Opportunities in...: June 23, 2010. Susanne Bolton, Committee Management Officer. BILLING CODE 7555-01-P ...

  19. Novel 16-membered macrolides modified at C-12 and C-13 positions of midecamycin A1 and miokamycin. Part 1: Synthesis and evaluation of 12,13-carbamate and 12-arylalkylamino-13-hydroxy analogues.

    PubMed

    Miura, Tomoaki; Kurihara, Ken-ichi; Furuuchi, Takeshi; Yoshida, Takuji; Ajito, Keiichi

    2008-04-01

    Design and synthesis of 16-membered macrolides modified at the C-12 and 13 positions are described. The compounds we report here have an arylalkylamino group attached to the C-12 position of the macrolactone. Both types of derivatives, 12,13-cyclic carbamates and non-carbamate analogues, were synthesized via 12-amino-13-hydroxy intermediates derived from 12,13-epoxide that was prepared by selective epoxidation at the C-12 and C-13 positions. 4'-Hydroxyl analogues were also prepared by acidic hydrolysis of a neutral sugar. These compounds were evaluated for in vitro antibacterial activity against respiratory tract pathogens. Some of these analogues exhibited an improved activity compared with the corresponding parent compound.

  20. Public affairs committee actions

    NASA Astrophysics Data System (ADS)

    The AGU Public Affairs Committee will create an ad hoc committee to consider possible AGU position statements concerning the effects of nuclear war.The action was taken at the May 31, 1983, meeting of the Committee at the AGU Spring Meeting in Baltimore. Present were Carroll Ann Hodges, Chairman, and members Thomas J. Ahrens, David Cauffman, Jared Cohon, Stamatios Krimigis, Robert Murphy, Raymond Roble, and George Shaw. Also attending were the current Congressional Fellow Arthur Weissman and SPR—Cosmic Rays Section Secretary Miriam Forman.

  1. Clinical ethics committee.

    PubMed Central

    Thornton, J. G.; Lilford, R. J.

    1995-01-01

    An informal clinical ethics committee was set up to advise on ethical problems in prenatal diagnosis in Leeds. It was used twice in six months but was not called on again in the subsequent year, and we describe this experience. In North America similar committees are often used to advise on clinical moral dilemmas, and we review the published evidence from there and discuss some of the advantages and problems. Our committee's advice may have altered clinicians' actions considerably, but perhaps doctors in Britain are not yet ready to surrender this aspect of clinical autonomy. PMID:7549638

  2. Fabrication of PLGA/MWNTs composite electrospun fibrous scaffolds for improved myogenic differentiation of C2C12 cells.

    PubMed

    Xu, Jiazhu; Xie, Ya; Zhang, Hongbo; Ye, Zhaoyang; Zhang, Wenjun

    2014-11-01

    Electrically conducting scaffolds have attracted tremendous attention in skeletal muscle tissue engineering. In this paper, poly(lactic-co-glycolic acid) (PLGA)/multi-wall carbon nanotubes (MWNTs) composite fibrous scaffolds were fabricated using the electrospinning technique. The physical properties of the composite fibers were characterized and proliferation and differentiation of C2C12 cells on these scaffolds were examined. It was found that the addition of MWNTs modulated the physical properties of PLGA fibers including morphology, fiber diameter, degradation, tensile strength and electrical conductivity, depending on the amount of MWNTs. These fibrous scaffolds were cytocompatible and supported the proliferation of C2C12 cells. Importantly, C2C12 cells showed more mature myotube formation on PLGA/MWNTs composite fibrous scaffolds compared to PLGA scaffolds. These results indicate that PLGA/MWNTs composite electrospun fibers have great potential in skeletal muscle tissue engineering.

  3. Downregulation of lipin-1 induces insulin resistance by increasing intracellular ceramide accumulation in C2C12 myotubes.

    PubMed

    Huang, Shujuan; Huang, Suling; Wang, Xi; Zhang, Qingli; Liu, Jia; Leng, Ying

    2017-01-01

    Dysregulation of lipid metabolism in skeletal muscle is involved in the development of insulin resistance. Mutations in lipin-1, a key lipid metabolism regulator leads to significant systemic insulin resistance in fld mice. However, the function of lipin-1 on lipid metabolism and insulin sensitivity in skeletal muscle is still unclear. Herein we demonstrated that downregulation of lipin-1 in C2C12 myotubes by siRNA transfection suppressed insulin action, characterized by reduced insulin stimulated Akt phosphorylation and glucose uptake. Correspondingly, decreased lipin-1 expression was observed in palmitate-induced insulin resistance in C2C12 myotubes, suggested that lipin-1 might play a role in the etiology of insulin resistance in skeletal muscle. The insulin resistance induced by lipin-1 downregulation was related to the disturbance of lipid homeostasis. Lipin-1 silencing reduced intracellular DAG and TAG levels, but elevated ceramide accumulation in C2C12 myotubes. Moreover, the impaired insulin stimulated Akt phosphorylation and glucose uptake caused by lipin-1 silencing could be blocked by the pretreatment with SPT inhibitor myriocin, ceramide synthase inhibitor FB1, or PP2A inhibitor okadaic acid, suggested that the increased ceramide accumulation might be responsible for the development of insulin resistance induced by lipin-1 silencing in C2C12 myotubes. Meanwhile, decreased lipin-1 expression also impaired mitochondrial function in C2C12 myotubes. Therefore, our study suggests that lipin-1 plays an important role in lipid metabolism and downregulation of lipin-1 induces insulin resistance by increasing intracellular ceramide accumulation in C2C12 myotubes. These results offer a molecular insight into the role of lipin-1 in the development of insulin resistance in skeletal muscle.

  4. Low Glucose but Not Galactose Enhances Oxidative Mitochondrial Metabolism in C2C12 Myoblasts and Myotubes

    PubMed Central

    Elkalaf, Moustafa; Anděl, Michal; Trnka, Jan

    2013-01-01

    Background Substituting galactose for glucose in cell culture media has been suggested to enhance mitochondrial metabolism in a variety of cell lines. We studied the effects of carbohydrate availability on growth, differentiation and metabolism of C2C12 myoblasts and myotubes. Methodology/Principal Findings We measured growth rates, ability to differentiate, citrate synthase and respiratory chain activities and several parameters of mitochondrial respiration in C2C12 cells grown in media with varying carbohydrate availability (5 g/l glucose, 1 g/l glucose, 1 g/l galactose, and no added carbohydrates). C2C12 myoblasts grow more slowly without glucose irrespective of the presence of galactose, which is not consumed by the cells, and they fail to differentiate without glucose in the medium. Cells grown in a no-glucose medium (with or without galactose) have lower maximal respiration and spare respiratory capacity than cells grown in the presence of glucose. However, increasing glucose concentration above physiological levels decreases the achievable maximal respiration. C2C12 myotubes differentiated at a high glucose concentration showed higher dependency on oxidative respiration under basal conditions but had lower maximal and spare respiratory capacity when compared to cells differentiated under low glucose condition. Citrate synthase activity or mitochondrial yield were not significantly affected by changes in the available substrate concentration but a trend towards a higher respiratory chain activity was observed at reduced glucose levels. Conclusions/Significance Our results show that using galactose to increase oxidative metabolism may not be applicable to every cell line, and the changes in mitochondrial respiratory parameters associated with treating cells with galactose are mainly due to glucose deprivation. Moderate concentrations of glucose (1 g/l) in a growth medium are optimal for mitochondrial respiration in C2C12 cell line while supraphysiological

  5. Downregulation of lipin-1 induces insulin resistance by increasing intracellular ceramide accumulation in C2C12 myotubes

    PubMed Central

    Huang, Shujuan; Huang, Suling; Wang, Xi; Zhang, Qingli; Liu, Jia; Leng, Ying

    2017-01-01

    Dysregulation of lipid metabolism in skeletal muscle is involved in the development of insulin resistance. Mutations in lipin-1, a key lipid metabolism regulator leads to significant systemic insulin resistance in fld mice. However, the function of lipin-1 on lipid metabolism and insulin sensitivity in skeletal muscle is still unclear. Herein we demonstrated that downregulation of lipin-1 in C2C12 myotubes by siRNA transfection suppressed insulin action, characterized by reduced insulin stimulated Akt phosphorylation and glucose uptake. Correspondingly, decreased lipin-1 expression was observed in palmitate-induced insulin resistance in C2C12 myotubes, suggested that lipin-1 might play a role in the etiology of insulin resistance in skeletal muscle. The insulin resistance induced by lipin-1 downregulation was related to the disturbance of lipid homeostasis. Lipin-1 silencing reduced intracellular DAG and TAG levels, but elevated ceramide accumulation in C2C12 myotubes. Moreover, the impaired insulin stimulated Akt phosphorylation and glucose uptake caused by lipin-1 silencing could be blocked by the pretreatment with SPT inhibitor myriocin, ceramide synthase inhibitor FB1, or PP2A inhibitor okadaic acid, suggested that the increased ceramide accumulation might be responsible for the development of insulin resistance induced by lipin-1 silencing in C2C12 myotubes. Meanwhile, decreased lipin-1 expression also impaired mitochondrial function in C2C12 myotubes. Therefore, our study suggests that lipin-1 plays an important role in lipid metabolism and downregulation of lipin-1 induces insulin resistance by increasing intracellular ceramide accumulation in C2C12 myotubes. These results offer a molecular insight into the role of lipin-1 in the development of insulin resistance in skeletal muscle. PMID:28123341

  6. Role of androgen receptor on cyclic mechanical stretch-regulated proliferation of C2C12 myoblasts and its upstream signals: IGF-1-mediated PI3K/Akt and MAPKs pathways.

    PubMed

    Ma, Yiming; Fu, Shaoting; Lu, Lin; Wang, Xiaohui

    2017-07-15

    To detect the effects of androgen receptor (AR) on cyclic mechanical stretch-modulated proliferation of C2C12 myoblasts and its pathways: roles of IGF-1, PI3K and MAPK. C2C12 were randomly divided into five groups: un-stretched control, six or 8 h of fifteen percent stretch, and six or 8 h of twenty percent stretch. Cyclic mechanical stretch of C2C12 were completed using a computer-controlled FlexCell Strain Unit. Cell proliferation and IGF-1 concentration in medium were detected by CCK8 and ELISA, respectively. Expressions of AR and IGF-1R, and expressions and activities of PI3K, p38 and ERK1/2 in stretched C2C12 cells were determined by Western blot. ①The proliferation of C2C12 cells, IGF-1 concentration in medium, expressions of AR and IGF-1R, and activities of PI3K, p38 and ERK1/2 were increased by 6 h of fifteen percent stretch, while decreased by twenty percent stretch for six or 8 h ②The fifteen percent stretch-increased proliferation of C2C12 cells was reversed by AR inhibitor, Flutamide. ③The increases of AR expression, activities of PI3K, p38 and ERK1/2 resulted from fifteen percent stretch were attenuated by IGF-1 neutralizing antibody, while twenty percent stretch-induced decreases of the above indicators were enhanced by recombinant IGF-1. ④Specific inhibitors of p38, ERK1/2 and PI3K all decreased the expression of AR in fifteen percent and twenty percent of stretched C2C12 cells. Cyclic mechanical stretch modulated the proliferation of C2C12 cells, which may be attributed to the alterations of AR via IGF-1-PI3K/Akt and IGF-1-MAPK (p38, ERK1/2) pathways in C2C12 cells. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. t10c12-CLA maintains higher bone mineral density during aging by modulating osteoclastogenesis and bone marrow adiposity

    PubMed Central

    Rahman, M; Halade, Ganesh V; Williams, Paul J; Fernandes, Gabriel

    2011-01-01

    Conjugated linoleic acid (CLA) has been shown to positively influence calcium and bone metabolism. Earlier, we showed that CLA (equal mixture of c9t11-CLA and t10c12-CLA) could protect age-associated bone loss by modulating inflammatory markers and osteoclastogenesis. Since, c9t11-CLA and t10c12-CLA isomers differentially regulate functional parameters and gene expression in different cell types, we examined the efficacy of individual CLA isomers against age-associated bone loss using 12 months old C57BL/6 female mice fed for 6 months with 10% corn oil (CO), 9.5% CO + 0.5% c9t11-CLA, 9.5% CO + 0.5% t10c12-CLA or 9.5% CO + 0.25% c9t11-CLA + 0.25% t10c12-CLA. Mice fed a t10c12-CLA diet maintained a significantly higher bone mineral density (BMD) in femoral, tibial and lumbar regions than those fed CO and c9t11-CLA diets as measured by dual-energy-x-ray absorptiometry (DXA). The increased BMD was accompanied by a decreased production of osteoclastogenic factors i.e. RANKL, TRAP5b, TNF-alpha and IL-6 in serum. Moreover, a significant reduction of high fat diet-induced bone marrow adiposity was observed in t10c12-CLA fed mice as compared to that of CO and c9t11-CLA fed mice, as measured by Oil-Red-O staining of bone marrow sections. In addition, a significant reduction of osteoclast differentiation and bone resorbing pit formation was observed in t10c12-CLA treated RAW 264.7 cell culture stimulated with RANKL as compared to that of c9t11-CLA and linoleic acid treated cultures. In conclusion, these findings suggest that t10c12-CLA is the most potent CLA isomer and it exerts its anti-osteoporotic effect by modulating osteoclastogenesis and bone marrow adiposity. PMID:21660964

  8. t10c12-CLA maintains higher bone mineral density during aging by modulating osteoclastogenesis and bone marrow adiposity.

    PubMed

    Rahman, Md M; Halade, Ganesh V; Williams, Paul J; Fernandes, Gabriel

    2011-09-01

    Conjugated linoleic acid (CLA) has been shown to positively influence calcium and bone metabolism. Earlier, we showed that CLA (equal mixture of c9t11-CLA and t10c12-CLA) could protect age-associated bone loss by modulating inflammatory markers and osteoclastogenesis. Since, c9t11-CLA and t10c12-CLA isomers differentially regulate functional parameters and gene expression in different cell types, we examined the efficacy of individual CLA isomers against age-associated bone loss using 12 months old C57BL/6 female mice fed for 6 months with 10% corn oil (CO), 9.5% CO + 0.5% c9t11-CLA, 9.5% CO + 0.5% t10c12-CLA or 9.5% CO + 0.25% c9t11-CLA + 0.25% t10c12-CLA. Mice fed a t10c12-CLA diet maintained a significantly higher bone mineral density (BMD) in femoral, tibial and lumbar regions than those fed CO and c9t11-CLA diets as measured by dual-energy-X-ray absorptiometry (DXA). The increased BMD was accompanied by a decreased production of osteoclastogenic factors, that is, RANKL, TRAP5b, TNF-alpha and IL-6 in serum. Moreover, a significant reduction of high fat diet-induced bone marrow adiposity was observed in t10c12-CLA fed mice as compared to that of CO and c9t11-CLA fed mice, as measured by Oil-Red-O staining of bone marrow sections. In addition, a significant reduction of osteoclast differentiation and bone resorbing pit formation was observed in t10c12-CLA treated RAW 264.7 cell culture stimulated with RANKL as compared to that of c9t11-CLA and linoleic acid treated cultures. In conclusion, these findings suggest that t10c12-CLA is the most potent CLA isomer and it exerts its anti-osteoporotic effect by modulating osteoclastogenesis and bone marrow adiposity.

  9. 76 FR 28759 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-18

    ... of Energy Efficiency and Renewable Energy Hydrogen and Fuel Cell Technical Advisory Committee (HTAC... meeting. SUMMARY: The Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) was established under... Updates Industry Presentations HTAC Subcommittee Overviews Fuel Cell Users Group State Initiatives...

  10. 75 FR 70215 - Environmental Technologies Trade Advisory Committee (ETTAC), Request for Nominations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... International Trade Administration Environmental Technologies Trade Advisory Committee (ETTAC), Request for... membership on the Environmental Technologies Trade Advisory Committee (ETTAC). SUMMARY: The Environmental... the Secretary of Commerce, the Environmental Trade Working Group (ETWG) of the Trade Promotion...

  11. 76 FR 62496 - Motor Carrier Safety Advisory Committee Series of Public Subcommittee Meetings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-07

    ... Federal Motor Carrier Safety Administration Motor Carrier Safety Advisory Committee Series of Public Subcommittee Meetings AGENCY: Federal Motor Carrier Safety Administration (FMCSA), DOT. ACTION: Notice of meeting. SUMMARY: The FMCSA's Motor Carrier Safety Advisory Committee (MCSAC) will hold working group...

  12. Organocatalytic Upgrading of Furfural and 5-Hydroxymethyl Furfural to C10 and C12 Furoins with Quantitative Yield and Atom-Efficiency

    PubMed Central

    Zang, Hongjun; Chen, Eugene Y. X.

    2015-01-01

    There is increasing interest in the upgrading of C5 furfural (FF) and C6 5-hydroxymethyl furfural (HMF) into C10 and C12 furoins as higher energy-density intermediates for renewable chemicals, materials, and biofuels. This work utilizes the organocatalytic approach, using the in situ generated N,S-heterocyclic carbene catalyst derived from thiazolium ionic liquids (ILs), to achieve highly efficient self-coupling reactions of FF and HMF. Specifically, variations of the thiazolium IL structure have led to the most active and efficient catalyst system of the current series, which is derived from a new thiazolium IL carrying the electron-donating acetate group at the 5-ring position. For FF coupling by this IL (0.1 mol %, 60 °C, 1 h), when combined with Et3N, furoin was obtained in >99% yield. A 97% yield of the C12 furoin was also achieved from the HMF coupling by this catalyst system (10 mol % loading, 120 °C, 3 h). On the other hand, the thiazolium IL bearing the electron-withdrawing group at the 5-ring position is the least active and efficient catalyst. The mechanistic aspects of the coupling reaction by the thiazolium catalyst system have also been examined and a mechanism has been proposed. PMID:25830482

  13. Organocatalytic upgrading of furfural and 5-hydroxymethyl furfural to C10 and C12 furoins with quantitative yield and atom-efficiency.

    PubMed

    Zang, Hongjun; Chen, Eugene Y X

    2015-03-30

    There is increasing interest in the upgrading of C5 furfural (FF) and C6 5-hydroxymethyl furfural (HMF) into C10 and C12 furoins as higher energy-density intermediates for renewable chemicals, materials, and biofuels. This work utilizes the organocatalytic approach, using the in situ generated N,S-heterocyclic carbene catalyst derived from thiazolium ionic liquids (ILs), to achieve highly efficient self-coupling reactions of FF and HMF. Specifically, variations of the thiazolium IL structure have led to the most active and efficient catalyst system of the current series, which is derived from a new thiazolium IL carrying the electron-donating acetate group at the 5-ring position. For FF coupling by this IL (0.1 mol %, 60 °C, 1 h), when combined with Et3N, furoin was obtained in >99% yield. A 97% yield of the C12 furoin was also achieved from the HMF coupling by this catalyst system (10 mol % loading, 120 °C, 3 h). On the other hand, the thiazolium IL bearing the electron-withdrawing group at the 5-ring position is the least active and efficient catalyst. The mechanistic aspects of the coupling reaction by the thiazolium catalyst system have also been examined and a mechanism has been proposed.

  14. Coral 13C/12C records of vertical seafloor displacement during megathrust earthquakes west of Sumatra

    NASA Astrophysics Data System (ADS)

    Gagan, Michael K.; Sosdian, Sindia M.; Scott-Gagan, Heather; Sieh, Kerry; Hantoro, Wahyoe S.; Natawidjaja, Danny H.; Briggs, Richard W.; Suwargadi, Bambang W.; Rifai, Hamdi

    2015-12-01

    The recent surge of megathrust earthquakes and tsunami disasters has highlighted the need for a comprehensive understanding of earthquake cycles along convergent plate boundaries. Space geodesy has been used to document recent crustal deformation patterns with unprecedented precision, however the production of long paleogeodetic records of vertical seafloor motion is still a major challenge. Here we show that carbon isotope ratios (δ13C) in the skeletons of massive Porites corals from west Sumatra record abrupt changes in light exposure resulting from coseismic seafloor displacements. Validation of the method is based on the coral δ13C response to uplift (and subsidence) produced by the March 2005 Mw 8.6 Nias-Simeulue earthquake, and uplift further south around Sipora Island during a M ∼ 8.4 megathrust earthquake in February 1797. At Nias, the average step-change in coral δ13C was 0.6 ± 0.1 ‰ /m for coseismic displacements of +1.8 m and -0.4 m in 2005. At Sipora, a distinct change in Porites microatoll growth morphology marks coseismic uplift of 0.7 m in 1797. In this shallow water setting, with a steep light attenuation gradient, the step-change in microatoll δ13C is 2.3 ‰ /m, nearly four times greater than for the Nias Porites. Considering the natural variability in coral skeletal δ13C, we show that the lower detection limit of the method is around 0.2 m of vertical seafloor motion. Analysis of vertical displacement for well-documented earthquakes suggests this sensitivity equates to shallow events exceeding Mw ∼ 7.2 in central megathrust and back-arc thrust fault settings. Our findings indicate that the coral 13C /12C paleogeodesy technique could be applied to convergent tectonic margins throughout the tropical western Pacific and eastern Indian oceans, which host prolific coral reefs, and some of the world's greatest earthquake catastrophes. While our focus here is the link between coral δ13C, light exposure and coseismic crustal deformation, the

  15. Multiple AMPK activators inhibit l-carnitine uptake in C2C12 skeletal muscle myotubes.

    PubMed

    Shaw, Andy; Jeromson, Stewart; Watterson, Kenneth R; Pediani, John D; Gallagher, Iain J; Whalley, Tim; Dreczkowski, Gillian; Brooks, Naomi; Galloway, Stuart D; Hamilton, D Lee

    2017-06-01

    Mutations in the gene that encodes the principal l-carnitine transporter, OCTN2, can lead to a reduced intracellular l-carnitine pool and the disease Primary Carnitine Deficiency. l-Carnitine supplementation is used therapeutically to increase intracellular l-carnitine. As AMPK and insulin regulate fat metabolism and substrate uptake, we hypothesized that AMPK-activating compounds and insulin would increase l-carnitine uptake in C2C12 myotubes. The cells express all three OCTN transporters at the mRNA level, and immunohistochemistry confirmed expression at the protein level. Contrary to our hypothesis, despite significant activation of PKB and 2DG uptake, insulin did not increase l-carnitine uptake at 100 nM. However, l-carnitine uptake was modestly increased at a dose of 150 nM insulin. A range of AMPK activators that increase intracellular calcium content [caffeine (10 mM, 5 mM, 1 mM, 0.5 mM), A23187 (10 μM)], inhibit mitochondrial function [sodium azide (75 μM), rotenone (1 μM), berberine (100 μM), DNP (500 μM)], or directly activate AMPK [AICAR (250 μM)] were assessed for their ability to regulate l-carnitine uptake. All compounds tested significantly inhibited l-carnitine uptake. Inhibition by caffeine was not dantrolene (10 μM) sensitive despite dantrolene inhibiting caffeine-mediated calcium release. Saturation curve analysis suggested that caffeine did not competitively inhibit l-carnitine transport. To assess the potential role of AMPK in this process, we assessed the ability of the AMPK inhibitor Compound C (10 μM) to rescue the effect of caffeine. Compound C offered a partial rescue of l-carnitine uptake with 0.5 mM caffeine, suggesting that AMPK may play a role in the inhibitory effects of caffeine. However, caffeine likely inhibits l-carnitine uptake by alternative mechanisms independently of calcium release. PKA activation or direct interference with transporter function may play a role. Copyright © 2017 the American Physiological Society.

  16. 40 CFR 721.10218 - 2-Propenoic acid, 2-mehtyl-, C12-15-branched and linear alkyl esters, telomers with alkyl 2...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false 2-Propenoic acid, 2-mehtyl-, C12-15... 2-Propenoic acid, 2-mehtyl-, C12-15-branched and linear alkyl esters, telomers with alkyl 2- thio]-2... identified generically as 2-propenoic acid, 2-methyl-, C12-15-branched and linear alkyl esters, telomers with...

  17. 40 CFR 721.10283 - Poly[oxy(methyl-1,2-ethanediyl)], .alpha.-sulfo-.omega.-hydroxy-, C12-13-branched and linear...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-, C12-13-branched and linear alkyl ethers, sodium salts. 721.10283 Section 721.10283 Protection of...-.omega.-hydroxy-, C12-13-branched and linear alkyl ethers, sodium salts. (a) Chemical substance and...-.omega.-hydroxy-, C12-13-branched and linear alkyl ethers, sodium salts (PMN P-10-486; CAS No....

  18. 40 CFR 721.3152 - Ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl sulfates (salts). 721.3152 Section 721... Ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl sulfates... ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl...

  19. 40 CFR 721.3152 - Ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl sulfates (salts). 721.3152 Section 721... Ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl sulfates... ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl...

  20. 40 CFR 721.3152 - Ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl sulfates (salts). 721.3152 Section 721... Ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl sulfates... ethanaminium, N-ethyl-2-hydroxy-N,N-bis(2-hydroxyethyl)-, diester with C12-18 fatty acids, ethyl...

  1. 76 FR 22395 - Federal Advisory Committee Act; Open Internet Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-21

    ... COMMISSION Federal Advisory Committee Act; Open Internet Advisory Committee AGENCY: Federal Communications... ``Open Internet Advisory Committee'' (hereinafter ``the Committee''), is being established. FOR FURTHER... Internet rules (available at...

  2. Intensity and linewidth measurements in the 13.7-micron fundamental bands of (C-12)2H2 and (C-12)(C-13)H2 at planetary atmospheric temperatures

    NASA Technical Reports Server (NTRS)

    Varanasi, Prasad

    1992-01-01

    The absolute intensities and collision-broadened half-widths of several lines in the P-,Q-, and R-branches of the nu5-fundamental band of (C-12)2H2 have been measured at various temperatures between 147 and 295 K employing the Doppler-limited spectral resolution (about 10 exp -4/cm) of a tunable diode laser spectrometer. The absolute intensities of R(5), R(7), R(9), and R(20) in the same fundamental belonging to (C-12)(C-13)H2 have also been measured at 294 K. The temperature dependence of the collision-broadened half-width has been determined for some of the lines broadened by planetary atmospheric gases, namely, He, Ar, H2, and N2. Four self-broadened linewidths of (C-12)2H2, as well as three H2-broadened linewidths and a self-broadened half-width of (C-12)(C-13)H2, have also been retrieved from the measurements.

  3. PPARγ regulates inflammatory reaction by inhibiting the MAPK/NF-κB pathway in C2C12 skeletal muscle cells.

    PubMed

    Kim, Jeong-Seok; Lee, Young-Hee; Chang, Yong-Uoo; Yi, Ho-Keun

    2017-02-01

    Excessive exercise induces an inflammatory response caused by oxidative stress, which delays recovery of damaged muscle fibers. The reduction of inflammatory response is important for skeletal muscle homeostasis. Peroxisome proliferator-activated receptor gamma (PPARγ) is an anti-inflammatory molecule, but the role of PPARγ in skeletal muscle as anti-inflammatory activity is not clear. Thus, this study examined the anti-inflammatory role of PPARγ against H2O2-induced oxidative stress in skeletal muscle. Sprague Dawley (SD) rats were exercised on a treadmill to induce oxidative stress. In vitro oxidative stress was evaluated in differentiated C2C12 cells stimulated using 200 μM H2O2. Inflammation-related molecules were determined by immunohistochemistry and Western blot analysis. Expressions of the inflammatory molecules tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), cyclooxygenase-2 (COX-2), and matrix metalloproteinase-2 (MMP-2) in muscles of the acute exercise group were highly increased. PPARγ was also highly expressed in these muscles. These inflammatory molecules were also markedly increased in C2C12 cells with H2O2 stimulation. However, PPARγ overexpression in C2C12 transfected by Ad/PPARγ dramatically reduced the inflammatory molecules. PPARγ also enhanced the anti-oxidants molecules like Cu/Zn-SOD, Mn-SOD, and hemeoxygenase-1 by reducing the generation of ROS, even in the presence of H2O2. PPARγ displayed dual anti-inflammatory and anti-oxidant roles by inhibiting the mitogen-activated protein kinase (MAPK) pathway and translocation of nuclear transcriptional factor-κB (NF-κB) from the cytosol to the nucleus. These results demonstrate a potential role of PPARγ in protecting muscle fibers against oxidative stress caused by excessive acute exercise due to its anti-inflammatory and anti-oxidant activity exerted by inhibition of the MAPK/NF-κB pathway.

  4. [Indoor air guide values for naphthalene and naphthalene-like compounds. Announcement of the German Ad-hoc Working Group on Indoor Guidelines of the Indoor Air Hygiene Committee and of the States' Supreme Health Authorities].

    PubMed

    2013-10-01

    The German Ad-hoc Working Group on Indoor Guidelines of the Indoor Air Hygiene Committee and of the States' Supreme Health Authorities is issuing indoor air guide values to protect public health. Naphthalene is a potentially volatile two-ring hydrocarbon with a mothball-like odor. Indoor air contaminations usually originate from tar-containing building products, sometimes from the use of mothballs. In Germany, indoor air concentrations of naphthalene are usually low, near the detection limit (medians of about 0.001 mg/m3, 95th percentiles up to 0.004 mg/m3). Naphthalene-like volatile compounds have been defined to cover methyl- and dimethylnaphthalenes and tricyclic aromatic hydrocarbons (e.g., acenaphthene, acenaphthylene, anthracene, fluorene and phenanthrene). Though methylnaphthalenes and dimethylnaphthalenes usually show low indoor air concentrations, they have been suspected to add to the mothball-like odor. Tricyclic aromatic hydrocarbons mostly occur below 0.001 mg/m3 of indoor air. Against this background naphthalene is seen to be the key component of this group of substances in indoor air. No valid human data is available with respect to health effects of inhaled naphthalene. Based on animal data cytotoxic-inflammatory lesions in the rat nasal epithelium are regarded as the critical endpoint. In a subchronic inhalation study in rats (Dodd et al., Inhal Toxicol 24:70–79, 2012), minimal effects were observed following an exposure to 5 mg naphthalene/m3. From this study the Ad-hoc Working Group derived a chronic NAEC of 2.5 mg naphthalene/m3. Time scaling was considered by a factor of 5.6 extrapolating from 6 to 24 h and 5 to 7 days, a factor of 2 applied for the use of F344 rats instead of the more sensitive Sprague-Dawley rats. Incorporating an interspecies factor of 1, an intraspecies factor of 10 and a factor of 2 for insufficient data on the toxicity of naphthalene in children resulted in a precautionary value of 0.01 mg naphthalene/m3 and a hazard

  5. Interagency Ocean Observing Committee

    NASA Astrophysics Data System (ADS)

    Rome, N. A.

    2013-12-01

    Decades of focused investment in ocean observing and prediction have produced many examples of substantive societal and economic benefit resulting from improved knowledge of ocean and coastal waters and their behavior. Many complex and difficult questions about the ocean remain, including many that have implications for the lives and livelihoods of millions of Americans. The United States has embarked on a series of efforts to develop an ocean observing system capable of addressing broad societal needs. This system is known as the U.S. Integrated Ocean Observing System (U.S. IOOS). The activities and members of the U.S. IOOS community are broad and complex. There are 18 Federal agencies involved in the U.S. IOOS program, as well as 11 U.S. IOOS Regional Associations that encompass efforts focused in U.S. coastal waters, the Great Lakes, and U.S. territories and their waters in the Pacific and the Caribbean. In addition, there are many Federal and academic scientists representing the U.S. Government in various United Nations-sponsored groups that plan and oversee global ocean observation programs. This diverse community is managed largely through cooperation rather than clear directive or budgetary authority, which has contributed to both the strong growth, and the integration weaknesses, of the U.S. IOOS program. The Interagency Ocean Observation Committee (IOOC) is a dynamic group of federal leaders required by Congress to implement procedural, technical, and scientific requirements to ensure full execution of U.S. IOOS. A major focus for the next decade of the IOOC is to help guide comprehensive processes that more fully integrate the requirements, technologies, data/product development and dissemination, testing and modeling efforts across the regional, national, and global sectors of the U.S. IOOS program. This poster/presentation will increase awareness of IOOC efforts to coordinate physical, chemical, and biological observations -- a complementary objective

  6. 75 FR 18487 - Committee Termination and Committee Establishment-Department of Defense Federal Advisory Committees

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-12

    ... of the Secretary Committee Termination and Committee Establishment--Department of Defense Federal Advisory Committees AGENCY: Department of Defense (DoD). ACTION: Committee termination and establishment...: the establishment of the Board of Advisors to the Presidents of the Naval Postgraduate School and the...

  7. Secretion of adenylate kinase 1 is required for extracellular ATP synthesis in C2C12 myotubes

    PubMed Central

    Choo, Hyo-Jung; Kim, Bong-Woo; Kwon, Oh-Bong; Lee, Chang Seok; Choi, Jong-Soon

    2008-01-01

    Extracellular ATP (exATP) has been known to be a critical ligand regulating skeletal muscle differentiation and contractibility. ExATP synthesis was greatly increased with the high level of adenylate kinase 1 (AK1) and ATP synthase β during C2C12 myogenesis. The exATP synthesis was abolished by the knock-down of AK1 but not by that of ATP synthase β in C2C12 myotubes, suggesting that AK1 is required for exATP synthesis in myotubes. However, membrane-bound AK1β was not involved in exATP synthesis because its expression level was decreased during myogenesis in spite of its localization in the lipid rafts that contain various kinds of receptors and mediate cell signal transduction, cell migration, and differentiation. Interestingly, cytoplasmic AK1 was secreted from C2C12 myotubes but not from C2C12 myoblasts. Taken together all these data, we can conclude that AK1 secretion is required for the exATP generation in myotubes. PMID:18446060

  8. Creatine supplementation prevents the inhibition of myogenic differentiation in oxidatively injured C2C12 murine myoblasts.

    PubMed

    Sestili, Piero; Barbieri, Elena; Martinelli, Chiara; Battistelli, Michela; Guescini, Michele; Vallorani, Luciana; Casadei, Lucia; D'Emilio, Alessandra; Falcieri, Elisabetta; Piccoli, Giovanni; Agostini, Deborah; Annibalini, Giosuè; Paolillo, Marco; Gioacchini, Anna Maria; Stocchi, Vilberto

    2009-09-01

    Creatine (Cr), one of the most popular nutritional supplements among athletes, has been recently shown to prevent the cytotoxicity caused by different oxidative stressors in various mammalian cell lines, including C2C12 myoblasts, via a direct antioxidant activity. Here, the effect of Cr on the differentiating capacity of C2C12 cells exposed to H(2)O(2) has been investigated. Differentiation into myotubes was monitored using morphological, ultrastructural, and molecular techniques. Treatment with H(2)O(2) (1 h) not only caused a significant (30%) loss of cell viability, but also abrogated the myogenic ability of surviving C2C12. Cr-supplementation (24 h prior to H(2)O(2) treatment) was found to prevent these effects. Interestingly, H(2)O(2)-challenged cells preconditioned with the established antioxidants trolox or N-acetyl-cysteine, although cytoprotected, did not display the same differentiating ability characterizing oxidatively-injured, Cr-supplemented cells. Besides acting as an antioxidant, Cr increased the level of muscle regulatory factors and IGF1 (an effect partly refractory to oxidative stress), the cellular availability of phosphocreatine and seemed to exert some mitochondrially-targeted protective activity. It is concluded that Cr preserves the myogenic ability of oxidatively injured C2C12 via a pleiotropic mechanism involving not only its antioxidant capacity, but also the contribution to cell energy charge and effects at the transcriptional level which common bona fide antioxidants lack.

  9. 26 CFR 1.381(c)(12)-1 - Recovery of bad debts, prior taxes, or delinquency amounts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 4 2011-04-01 2011-04-01 false Recovery of bad debts, prior taxes, or...(c)(12)-1 Recovery of bad debts, prior taxes, or delinquency amounts. (a) Carryover requirement. (1... corporation is entitled to the recovery of a bad debt, prior tax, or delinquency amount on account of which...

  10. 26 CFR 1.381(c)(12)-1 - Recovery of bad debts, prior taxes, or delinquency amounts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 4 2010-04-01 2010-04-01 false Recovery of bad debts, prior taxes, or...(c)(12)-1 Recovery of bad debts, prior taxes, or delinquency amounts. (a) Carryover requirement. (1... corporation is entitled to the recovery of a bad debt, prior tax, or delinquency amount on account of which...

  11. Wnt/{beta}-catenin signaling changes C2C12 myoblast proliferation and differentiation by inducing Id3 expression

    SciTech Connect

    Zhang, Long; Shi, Songting; Zhang, Juan; Zhou, Fangfang; Dijke, Peter ten

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer Expression of Id3 but not Id1 is induced by Wnt3a stimulation in C2C12 cells. Black-Right-Pointing-Pointer Wnt3a induces Id3 expression via canonical Wnt/{beta}-catenin pathway. Black-Right-Pointing-Pointer Wnt3a-induced Id3 expression does not depend on BMP signaling activation. Black-Right-Pointing-Pointer Induction of Id3 expression is critical determinant in Wnt3a-induced cell proliferation and differentiation. -- Abstract: Canonical Wnt signaling plays important roles in regulating cell proliferation and differentiation. In this study, we report that inhibitor of differentiation (Id)3 is a Wnt-inducible gene in mouse C2C12 myoblasts. Wnt3a induced Id3 expression in a {beta}-catenin-dependent manner. Bone morphogenetic protein (BMP) also potently induced Id3 expression. However, Wnt-induced Id3 expression occurred independent of the BMP/Smad pathway. Functional studies showed that Id3 depletion in C2C12 cells impaired Wnt3a-induced cell proliferation and alkaline phosphatase activity, an early marker of osteoblast cells. Id3 depletion elevated myogenin induction during myogenic differentiation and partially impaired Wnt3a suppressed myogenin expression in C2C12 cells. These results suggest that Id3 is an important Wnt/{beta}-catenin induced gene in myoblast cell fate determination.

  12. 26 CFR 1.381(c)(12)-1 - Recovery of bad debts, prior taxes, or delinquency amounts.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 4 2012-04-01 2012-04-01 false Recovery of bad debts, prior taxes, or... Reorganizations § 1.381(c)(12)-1 Recovery of bad debts, prior taxes, or delinquency amounts. (a) Carryover... corporation is entitled to the recovery of a bad debt, prior tax, or delinquency amount on account of which a...

  13. 26 CFR 1.381(c)(12)-1 - Recovery of bad debts, prior taxes, or delinquency amounts.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 4 2013-04-01 2013-04-01 false Recovery of bad debts, prior taxes, or... Reorganizations § 1.381(c)(12)-1 Recovery of bad debts, prior taxes, or delinquency amounts. (a) Carryover... corporation is entitled to the recovery of a bad debt, prior tax, or delinquency amount on account of which a...

  14. 26 CFR 1.381(c)(12)-1 - Recovery of bad debts, prior taxes, or delinquency amounts.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 4 2014-04-01 2014-04-01 false Recovery of bad debts, prior taxes, or... Reorganizations § 1.381(c)(12)-1 Recovery of bad debts, prior taxes, or delinquency amounts. (a) Carryover... corporation is entitled to the recovery of a bad debt, prior tax, or delinquency amount on account of which a...

  15. Flavones Inhibit LPS-Induced Atrogin-1/MAFbx Expression in Mouse C2C12 Skeletal Myotubes.

    PubMed

    Shiota, Chieko; Abe, Tomoki; Kawai, Nobuhiko; Ohno, Ayako; Teshima-Kondo, Shigetada; Mori, Hiroyo; Terao, Junji; Tanaka, Eiji; Nikawa, Takeshi

    2015-01-01

    Muscle atrophy is a complex process that occurs as a consequence of various stress events. Muscle atrophy-associated genes (atrogenes) such as atrogin-1/MAFbx and MuRF-1 are induced early in the atrophy process, and the increase in their expression precedes the loss of muscle weight. Although antioxidative nutrients suppress atrogene expression in skeletal muscle cells, the inhibitory effects of flavonoids on inflammation-induced atrogin-1/MAFbx expression have not been clarified. Here, we investigated the inhibitory effects of flavonoids on lipopolysaccharide (LPS)-induced atrogin-1/MAFbx expression. We examined whether nine flavonoids belonging to six flavonoid categories inhibited atrogin-1/MAFbx expression in mouse C2C12 myotubes. Two major flavones, apigenin and luteolin, displayed potent inhibitory effects on atrogin-1/MAFbx expression. The pretreatment with apigenin and luteolin significantly prevented the decrease in C2C12 myotube diameter caused by LPS stimulation. Importantly, the pretreatment of LPS-stimulated myoblasts with these flavones significantly inhibited LPS-induced JNK phosphorylation in C2C12 myotubes, resulting in the significant suppression of atrogin-1/MAFbx promoter activity. These results suggest that apigenin and luteolin, prevent LPS-mediated atrogin-1/MAFbx expression through the inhibition of the JNK signaling pathway in C2C12 myotubes. Thus, these flavones, apigenin and luteolin, may be promising agents to prevent LPS-induced muscle atrophy.

  16. Measurement of 13C/12C of chloroacetic acids by gas chromatography/combustion/isotope ratio mass spectrometry.

    PubMed

    Wong, Charles S; Muir, Derek C G; Mabury, Scott A

    2003-02-01

    This paper describes a novel analytical methodology using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) to measure the 13C/12C ratios of chloroacetic acids (CAAs). CAAs are a major class of environmental pollutants that are widely distributed throughout the world, often at relatively high concentrations, and are of concern due to their toxic effects, particularly on plants. The 13C/12C of CAA reagents was measured by IRMS subsequent to offline combustion. Aqueous solutions of these CAAs were derivatized to the corresponding methyl chloroacetates (MCAAs) with acidic methanol with a known isotopic composition, extracted into pentane, and analyzed by GC/C/IRMS. Measured 13C/12C ratios of derivatized MCAAs were in agreement with calculated values within 1 per thousand for monochloroacetic acid and trichloroacetic acid and within 2 per thousand for dichloroacetic acid, suggesting that methylation and other analytical methodology steps do not isotopically fractionate derivatized MCAAs. 13C/12C ratios of reagent CAAs from different sources had varying isotopic signatures suggesting differences in source carbon and/or production methods. Our results underscore the potential of stable isotopes to serve as tracers of environmental sources of CAAs.

  17. Insulin Sensitizing Effects of Oligomannuronate-Chromium (III) Complexes in C2C12 Skeletal Muscle Cells

    PubMed Central

    Wang, Wei; Han, Zhangrun; Li, Guangsheng; Zhang, Lijuan; Zhao, Xia; Yu, Guangli

    2011-01-01

    Background It was known that the insulin resistance in skeletal muscle is a major pathogenic factor in diabetes mellitus. Therefore prevention of metabolic disorder caused by insulin resistance and improvement of insulin sensitivity are very important for the therapy of type 2 diabetes. In the present study, we investigated the ability of marine oligosaccharides oligomannuronate and its chromium (III) complexes from brown alga to enhance insulin sensitivity in C2C12 skeletal muscle cells. Methodology/Principal Findings We demonstrated that oligomannuronate, especially its chromium (III) complexes, enhanced insulin-stimulated glucose uptake and increased the mRNA expression of glucose transporter 4 (GLUT4) and insulin receptor (IR) after their internalization into C2C12 skeletal muscle cells. Additionally, oligosaccharides treatment also significantly enhanced the phosphorylation of proteins involved in both AMP activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathways in C2C12 cells, indicating that the oligosaccharides activated both the insulin signal pathway and AMPK pathways as their mode of action. Moreover, oligosaccharides distributed to the mitochondria after internalization into C2C12 cells and increased the expression of transcriptional regulator peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), carnitine palmitoyl transferase-1 (CPT-1), and phosphorylated acetyl-CoA carboxylase (p-ACC), which suggested that the actions of these oligosaccharides might be associated with mitochondria through increasing energy expenditure. All of these effects of marine oligosaccharides were comparable to that of the established anti-diabetic drug, metformin. In addition, the treatment with oligosaccharides showed less toxicity than that of metformin. Conclusions/Significance Our findings indicate that oligomannuonate and its chromium (III) complexes improved insulin

  18. The C12(O16,γSi28) radiative capture reaction at sub-barrier energies

    NASA Astrophysics Data System (ADS)

    Goasduff, A.; Courtin, S.; Haas, F.; Lebhertz, D.; Jenkins, D. G.; Fallis, J.; Ruiz, C.; Hutcheon, D. A.; Amandruz, P.-A.; Davis, C.; Hager, U.; Ottewell, D.; Ruprecht, G.

    2014-01-01

    The heavy-ion radiative capture C12(O16,γSi28) was measured at the sub-Coulomb barrier bombarding energy Elab=15.7 MeV, which corresponds to the lowest important resonance observed in the C12+ O16 fusion excitation function. Thanks to combination of the bismuth germanate (BGO) γ-ray array and the 0∘ DRAGON electromagnetic spectrometer at TRIUMF, the γ-decay spectrum from the entrance channel down to the ground state of 28Si was measured. Comparisons of the experimental spectrum to γ spectrum extracted from Monte Carlo simulations of the complete setup suggest a Jπ=2+ spin-parity assignment to the entrance channel and yield the radiative capture cross section σRC=0.22±0.04μb. Combining this present spin assignment with previous data on radiative capture, a J (J+1) systematics was constructed, and it indicated a moment of inertia commensurate with the C12+O16 grazing angular momentum. Strong dipole transitions are observed from the entrance channel to T =1 states around 11.5 MeV and are found to result from enhanced M1IV transitions to states exhausting a large part of the M1 sum rule built on the ground state of 28Si. This specific decay was also reported at bombarding energies close to the Coulomb barrier in our previous study of the C12(C12,γ24Mg) heavy-ion radiative capture reaction. Similarities between both systems are investigated.

  19. Palmitate increases musclin gene expression through activation of PERK signaling pathway in C2C12 myotubes.

    PubMed

    Gu, Ning; Guo, Qian; Mao, Ke; Hu, Hailong; Jin, Sanli; Zhou, Ying; He, Hongjuan; Oh, Yuri; Liu, Chuanpeng; Wu, Qiong

    2015-11-20

    Musclin is a type of muscle-secreted cytokine and its increased gene expression induces insulin resistance in type 2 diabetes. However, the mechanism underlying increased musclin gene expression is currently unclear. Excessive saturated fatty acids (SFA) can activate the secretion of several muscle-secreted cytokines as well as endoplasmic reticulum (ER) stress pathway, thereby contributing to the development of type 2 diabetes. The purpose of this study was to investigate the mechanisms responsible for the effect of palmitate, the most abundant SFA in the plasma, on the gene expression of musclin in C2C12 myotubes. Treatment of C2C12 myotubes with palmitate or tunicamycin significantly increased the expression of musclin as well as ER stress-related genes, but treatment with oleate did not. Pre-treatment of C2C12 myotubes with 4-phenyl butyrate suppressed the expression of ER stress-related genes, simultaneously, resulting in decreased expression of the musclin gene induced by palmitate or tunicamycin. These results indicate that ER stress is related to palmitate-induced musclin gene expression. Moreover, palmitate-induced musclin gene expression was significantly inhibited in C2C12 myotubes when PERK pathway signaling was suppressed by knockdown of the PERK gene or treatment with GSK2656157, a PERK autophosphorylation inhibitor. However, there was no difference in the palmitate-induced musclin gene expression when IRE1 and ATF6 signaling pathways were suppressed by knockdown of the IRE1 and ATF6 genes. These findings suggest that palmitate increases musclin gene expression via the activation of the PERK signaling pathway in C2C12 myotubes.

  20. Youth Voice in Nigerian School-Based Management Committees

    ERIC Educational Resources Information Center

    Umar, Bashiru Bako; Krauss, Steven Eric; Samah, Asnarulkhadi Abu; Hamid, Jamaliah Abdul

    2017-01-01

    In Nigeria, School-Based Management Committees (SBMCs) aim to provide an opportunity for all stakeholders, particularly the vulnerable groups in the school's host communities such as young people and women to partake in school governance. Research on the experiences of youth voice in the committees is scant, however, as much of the existing…

  1. The untimely death of the UK Donation Ethics Committee.

    PubMed

    Shaw, David

    2017-01-01

    This brief report describes the contribution of the UK Donation Ethics Committee to organ donation and transplantation in the UK, and explains why the committee has met an early demise. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  2. 41 CFR 105-54.204 - Advisory committee membership.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... membership. 105-54.204 Section 105-54.204 Public Contracts and Property Management Federal Property... committee membership. (a) Advisory committees that GSA establishes represent the points of view of the... balance, the agency considers for membership a cross-section of interested persons and groups...

  3. 41 CFR 105-54.204 - Advisory committee membership.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... membership. 105-54.204 Section 105-54.204 Public Contracts and Property Management Federal Property... committee membership. (a) Advisory committees that GSA establishes represent the points of view of the... balance, the agency considers for membership a cross-section of interested persons and groups...

  4. 41 CFR 105-54.204 - Advisory committee membership.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... membership. 105-54.204 Section 105-54.204 Public Contracts and Property Management Federal Property... committee membership. (a) Advisory committees that GSA establishes represent the points of view of the... balance, the agency considers for membership a cross-section of interested persons and groups...

  5. 41 CFR 105-54.204 - Advisory committee membership.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... professional or personal qualifications or experience to contribute to the functions and tasks to be performed... particular individual or group to obtain different points of view relevant to committee business. The... advisory committee members. (c) Nominees for membership must submit a Statement of Employment and...

  6. Data monitoring committees: Promoting best practices to address emerging challenges.

    PubMed

    Fleming, Thomas R; DeMets, David L; Roe, Matthew T; Wittes, Janet; Calis, Karim A; Vora, Amit N; Meisel, Alan; Bain, Raymond P; Konstam, Marvin A; Pencina, Michael J; Gordon, David J; Mahaffey, Kenneth W; Hennekens, Charles H; Neaton, James D; Pearson, Gail D; Andersson, Tomas Lg; Pfeffer, Marc A; Ellenberg, Susan S

    2017-04-01

    Data monitoring committees are responsible for safeguarding the interests of study participants and assuring the integrity and credibility of clinical trials. The independence of data monitoring committees from sponsors and investigators is essential in achieving this mission. Creative approaches are needed to address ongoing and emerging challenges that potentially threaten data monitoring committees' independence and effectiveness. An expert panel of representatives from academia, industry and government sponsors, and regulatory agencies discussed these challenges and proposed best practices and operating principles for effective functioning of contemporary data monitoring committees. Prospective data monitoring committee members need better training. Options could include didactic instruction as well as apprenticeships to provide real-world experience. Data monitoring committee members should be protected against legal liability arising from their service. While avoiding breaches in confidentiality of interim data remains a high priority, data monitoring committees should have access to unblinded efficacy and safety data throughout the trial to enable informed judgments about risks and benefits. Because overly rigid procedures can compromise their independence, data monitoring committees should have the flexibility necessary to best fulfill their responsibilities. Data monitoring committee charters should articulate principles that guide the data monitoring committee process rather than list a rigid set of requirements. Data monitoring committees should develop their recommendations by consensus rather than through voting processes. The format for the meetings of the data monitoring committee should maintain the committee's independence and clearly establish the leadership of the data monitoring committee chair. The independent statistical group at the Statistical Data Analysis Center should have sufficient depth of knowledge about the study at hand and

  7. A one-dimensional double-chain coordination polymer: [Mn(C12H13NO6S)(C10H8N2)]n.

    PubMed

    Liang, Fu-Pei; Chen, Man-Sheng; Hu, Rui-Xiang; Chen, Zi-Lu

    2004-06-01

    In the title compound, poly[[(2,2'-bipyridine-kappa(2)N,N')manganese(II)]-micro(3)-N-tosyl-L-glutamato-kappa(4)O,O':O":O"'], [Mn(tsgluo)(bipy)](n), where tsgluo is N-tosyl-L-glutamate (C(12)H(13)NO(6)S) and bipy is 2,2'-bipyridine (C(10)H(8)N(2)), the Mn atoms are octahedrally coordinated by two N atoms of one bipy ligand and by four O atoms of three tsgluo(2-) anions. The gamma-carboxyl group coordinates to the Mn(II) atom in a chelating mode, while the alpha-carboxyl group coordinates in a bidentate-bridging mode. The complex displays a one-dimensional double-chain structure.

  8. Dopamine-Incorporated Dual Bioactive Electroactive Shape Memory Polyurethane Elastomers with Physiological Shape Recovery Temperature, High Stretchability, and Enhanced C2C12 Myogenic Differentiation.

    PubMed

    Zhao, Xin; Dong, Ruonan; Guo, Baolin; Ma, Peter X

    2017-09-06

    Soft tissue engineering needs elastic biomaterials not only mimicking the elasticity of soft tissue but also possessing multiple bioactivity to promote cell adhesion, proliferation, and differentiation, which still remain ongoing challenges. Herein, we synthesized a series of dopamine-incorporated dual bioactive electroactive shape memory polyurethane elastomers by combining the properties of elastomeric poly(citric acid-co-polycaprolactone) (CA-PCL) polyurethane elastomer, bioactive dopamine (DA), and electroactive aniline hexamer (AH). The chemical structures, electroactivity, conductivity, thermal properties, hydrophilicity and hydration ability, mechanical properties, and degradability of the polyurethane elastomers were systematically characterized. The elastomers showed excellent shape fixity ratio and shape recovery ability under physiological conditions. The elastomers' elongation and stress were tailored by the AH content, whereas the hydrophilicity and hydration ability of the elastomers were adjusted by the content of DA and AH, as well as the doping state of AH. The viability and proliferation results of C2C12 cells seeded on the elastomers showed their excellent cytocompatibility. Additionally, by analyzing the protein and gene level, the promotion effect on myogenic differentiation of C2C12 cells by these elastomers compared to that by control groups (PCL80 000, CA-PCL elastomer, and CA-PCL elastomer with the DA segment) was demonstrated. Furthermore, the results from subcutaneous implantation confirmed the elastomers' mild host response in vivo. These results represent that these dopamine-incorporated dual bioactive electroactive shape memory polyurethane elastomers are promising candidates for soft tissue regeneration that is sensitive to electrical signals.

  9. 11 CFR 110.3 - Contribution limitations for affiliated committees and political party committees; Transfers (2 U...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 11 CFR 100.5, shall be considered to be made or received by a single political committee. See 11 CFR... committees shall be considered to be made or received by a single political committee— (i) Authorized... corporation, labor organization, person or group of persons, including any parent, subsidiary, branch...

  10. ICAP 2010 COMMITTEES ICAP 2010 Committees

    NASA Astrophysics Data System (ADS)

    2011-01-01

    Co-Chairs Peter Hannaford, Hans Bachor Program Committee Hans BachorAustralia Sergei BagayevRussia Vanderlei BagnatoBrazil Rainer BlattAustria Nir DavidsonIsrael Gordon DrakeCanada Wolfgang ErtmerGermany Phillip GouldUSA Peter HannafordAustralia Ed HindsUK Massimo InguscioItaly Wonho JheKorea Hidetoshi KatoriJapan Daniel KleppnerUSA Michéle LeducFrance Maciej LewensteinSpain Margaret MurnaneUSA Eugene PolzikDenmark Gerhard RempeGermany Monika Ritsch-MarteAustria Sando StringariItaly Vladan VuleticUSA Xinye XuChina Local Organising Committee Hans BachorAustralian National University, Canberra Ken BaldwinAustralian National University, Canberra Peter DrummondSwinburne University of Technology, Melbourne Peter HannafordSwinburne University of Technology, Melbourne Andre LuitenUniversity of Western Australia, Perth Russell McLeanSwinburne University of Technology, Melbourne Halina Rubinsztein-DunlopUniversity of Queensland, Brisbane Robert SangGriffith University, Brisbane Robert ScholtenUniversity of Melbourne Andrew WilsonUniversity of Otago, New Zealand International Advisory Committee Ennio ArimondoItaly Vanderlei BagnatoBrazil Victor BalykinRussia Rainer BlattAustria Claude Cohen-TannoudjiFrance Robin CôtéUSA Gordon DrakeCanada Norval FortsonUSA Theodore HänschGermany Serge HarocheFrance Ed HindsUK Massimo InguscioItaly Wolfgang KetterleUSA Daniel KleppnerUSA Bill PhillipsUSA Lev PitaevskiiRussia David PritchardUSA Fujio ShimizuJapan Winthrop SmithUSA Jook WalravenThe Netherlands David WinelandUSA Tsutomu YabuzakiJapan

  11. 7 CFR 983.8 - Committee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE PISTACHIOS GROWN IN CALIFORNIA, ARIZONA, AND NEW MEXICO Definitions § 983.8 Committee. Committee means the Administrative Committee for...

  12. 7 CFR 983.8 - Committee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE PISTACHIOS GROWN IN CALIFORNIA, ARIZONA, AND NEW MEXICO Definitions § 983.8 Committee. Committee means the Administrative Committee for...

  13. 7 CFR 983.8 - Committee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE PISTACHIOS GROWN IN CALIFORNIA, ARIZONA, AND NEW MEXICO Definitions § 983.8 Committee. Committee means the Administrative Committee for...

  14. 7 CFR 945.11 - Committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN CERTAIN... Committee. Committee means the administrative committee, called the Idaho-Eastern Oregon Potato...

  15. 7 CFR 945.11 - Committee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN CERTAIN... Committee. Committee means the administrative committee, called the Idaho-Eastern Oregon Potato...

  16. 7 CFR 945.11 - Committee.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN CERTAIN... Committee. Committee means the administrative committee, called the Idaho-Eastern Oregon Potato...

  17. 7 CFR 932.18 - Committee.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE OLIVES GROWN IN CALIFORNIA Order Regulating Handling Definitions § 932.18 Committee. Committee means the California Olive Committee...

  18. 7 CFR 932.18 - Committee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE OLIVES GROWN IN CALIFORNIA Order Regulating Handling Definitions § 932.18 Committee. Committee means the California Olive Committee...

  19. 7 CFR 932.18 - Committee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE OLIVES GROWN IN CALIFORNIA Order Regulating Handling Definitions § 932.18 Committee. Committee means the California Olive Committee...

  20. 7 CFR 932.18 - Committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE OLIVES GROWN IN CALIFORNIA Order Regulating Handling Definitions § 932.18 Committee. Committee means the California Olive Committee...

  1. 7 CFR 932.18 - Committee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE OLIVES GROWN IN CALIFORNIA Order Regulating Handling Definitions § 932.18 Committee. Committee means the California Olive Committee...

  2. 7 CFR 966.15 - Committee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE TOMATOES GROWN IN FLORIDA Order Regulating Handling Definitions § 966.15 Committee. Committee means the Florida Tomato Committee, established...

  3. 7 CFR 966.15 - Committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TOMATOES GROWN IN FLORIDA Order Regulating Handling Definitions § 966.15 Committee. Committee means the Florida Tomato Committee, established...

  4. 7 CFR 966.15 - Committee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AND ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE TOMATOES GROWN IN FLORIDA Order Regulating Handling Definitions § 966.15 Committee. Committee means the Florida Tomato Committee, established...

  5. 7 CFR 966.15 - Committee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TOMATOES GROWN IN FLORIDA Order Regulating Handling Definitions § 966.15 Committee. Committee means the Florida Tomato Committee, established...

  6. 7 CFR 966.15 - Committee.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TOMATOES GROWN IN FLORIDA Order Regulating Handling Definitions § 966.15 Committee. Committee means the Florida Tomato Committee, established...

  7. 7 CFR 920.8 - Committee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE KIWIFRUIT GROWN IN CALIFORNIA Definitions § 920.8 Committee. Committee means the Kiwifruit Administrative Committee established pursuant to §...

  8. 7 CFR 920.8 - Committee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE KIWIFRUIT GROWN IN CALIFORNIA Definitions § 920.8 Committee. Committee means the Kiwifruit Administrative Committee established pursuant to §...

  9. 7 CFR 920.8 - Committee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE KIWIFRUIT GROWN IN CALIFORNIA Definitions § 920.8 Committee. Committee means the Kiwifruit Administrative Committee established pursuant to §...

  10. 7 CFR 920.8 - Committee.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE KIWIFRUIT GROWN IN CALIFORNIA Definitions § 920.8 Committee. Committee means the Kiwifruit Administrative Committee established pursuant to §...

  11. 7 CFR 920.8 - Committee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ORDERS; FRUITS, VEGETABLES, NUTS), DEPARTMENT OF AGRICULTURE KIWIFRUIT GROWN IN CALIFORNIA Definitions § 920.8 Committee. Committee means the Kiwifruit Administrative Committee established pursuant to §...

  12. Precision Machining Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in precision machining technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and…

  13. Fundamentals of Dental Assisting. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in dental assisting, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are used as…

  14. Applied Welding Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in applied welding technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are…

  15. Industrial Maintenance Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in industrial maintenance technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings,…

  16. Fundamentals of Dental Assisting. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in dental assisting, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are used as…

  17. Industrial Maintenance Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in industrial maintenance technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings,…

  18. Drafting & Design Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in drafting and design technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and…

  19. Applied Welding Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in applied welding technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and are…

  20. Drafting & Design Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in drafting and design technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and…

  1. Precision Machining Technology. Technical Committee Report.

    ERIC Educational Resources Information Center

    Idaho State Dept. of Education, Boise. Div. of Vocational Education.

    This Technical Committee Report prepared by industry representatives in Idaho lists the skills currently necessary for an employee in that state to obtain a job in precision machining technology, retain a job once hired, and advance in that occupational field. (Task lists are grouped according to duty areas generally used in industry settings, and…

  2. Committees and supporting organizations

    NASA Astrophysics Data System (ADS)

    2011-09-01

    Advisory Committee:Organizing Committee: Marcello Baldo (Catania)Takaharu Otsuka (Tokyo), co-chair George Bertsch (Seattle)Michael Urban (Orsay), co-chair Jean-Paul Blaizot (Saclay)Taiichi Yamada (Yokohama) Michel Girod (Bruyères-le-Châtel)Nguyen van Giai (Orsay) Hisashi Horiuchi (Osaka)Shinichiro Fujii (Tokyo) Umberto Lombardo (Catania)Jérôme Margueron (Orsay) Gerd Röpke (Rostock)Kouichi Hagino (Sendai) Hiroyuki Sagawa (Aizu)Yoshiko Kanada-En'yo (Kyoto) Piet Van Isacker (Caen) Enrico Vigezzi (Milano) IPN logo    EFES logo    CNRS logo    ihp logo

  3. [Hospital clinical ethics committees].

    PubMed

    Gómez Velásquez, Luis; Gómez Espinosa, Luis Néstor

    2007-01-01

    The scientific and technological advances have been surprising, more in the two last decades, but they don't go united with to the ethical values of the medical professional practice, it has been totally escaped, specially when the biological subsistence, the maintenance of the life through apparatuses and the mechanisms that prolong the existence are who undergoes an alteration that until recently time was mortal shortly lapse. It is common listening that exist a crisis in the medical profession, but what really is it of human values, which as soon and taken into nowadays, actually professional account, which gives rise to a dehumanization towards the life, the health, the disease, the suffering and the death. The ideal of the doctor to give to service to the man in its life and health, as well to be conscious that the last biological process that must fulfill is the death, and when it appears, does not have considered as a actually professional failure. It has protect to the patient as the extreme cruelty therapeutic, that it has right a worthy death. It's taking to the birth of the hospital ethics committees, they have like function to analyze, to advise and to think about the ethical dilemmas that appear actually clinical or in the biomedical investigation. In 1982 in the UEA only 1% of its hospitals had a ethics committees; by 1988, it was 67% and the 100% in 2000. In Mexico the process of the formation by these committees begins, only in the Military Central Hospital, to count the ethics committee on 1983, also the Hospital no. 14 of the IMSS in Guadalajara, it works with regularity from 1995, with internal teaching of bioethic. The Secretariat of Health has asked the formation of the bioethical committees in each hospital, and order the it was be coordinated by the National Committee of Bioética. The integration of these committees is indispensable that their members have the knowledge necessary of bioética. The Mexican Society of Ortopedia, conscious of

  4. Geomagnetism-Paleomagnetism Committee

    NASA Astrophysics Data System (ADS)

    Banerjee, Subir K.

    I n response to strong member concerns about the future of geomagnetism, Neil D. Opdyke, President of the Geomagnetism and Paleomagnetism Section, has appointed an ad hoc Committee for the Future of Geomagnetism and Paleomagnetism consisting of Subir K. Banerjee (chair; University of Minnesota, Minneapolis), Joseph Cain (U.S. Geological Survey, Denver, Colo.) and Rob Van der Voo (University of Michigan, Ann Arbor). The committee seeks, from the membership at large, perceptions of future directions of research, help in identifying a few expanding research areas that show a promise of delivering significant results in the next 5 to 10 years, and guidance in generating a strategy to bring these about.

  5. Publications committee highlights

    NASA Astrophysics Data System (ADS)

    On April 1 and 2, James C.G. Walker, chairman, and Publications Committee members Thomas Graedel, Jurate Landwehr, Peter Molnar, Bruce Taft, and Martin Walt met at AGU headquarters. The committee meets in these extended sessions at least twice a year to review policy matters and to prepare to advise the Council on the problems and opportunities facing the AGU publications program. As a result of the April meeting the following major actions will be taken: Recommend to Council that the experimental split of JGR-Oceans and Atmospheres be made permanent.

  6. Nominations sought for extractive industries committee

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    2012-08-01

    U.S. secretary of the Interior Ken Salazar announced on 26 July the establishment of a national committee to guide U.S. implementation of the Extractive Industries Transparency Initiative. The Interior Department is seeking nominations of people interested in serving on the committee, which will be convened as a new federal advisory group. The initiative calls on governments to publicly disclose revenues from oil, gas, and mining assets and for companies to make disclosures about payments. U.S. president Barack Obama announced the commitment of the United States to participate in this initiative as part of the U.S. National Action Plan for the International Open Government Partnership.

  7. 75 FR 3744 - National Advisory Committee on Rural Health and Human Services; Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-22

    ... by Committee discussion and overview from staff to the Committee. Following these presentations, Subcommittees will be selected and meet for small group discussions. There will be a review of the Subcommittee... delivery as well as workforce. Committee discussion on the issues and an overview of rest of the meeting...

  8. 76 FR 20051 - Advisory Committee for International Science & Engineering; Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ... Advisory Committee for International Science & Engineering; Notice of Meeting In accordance with Federal... following meeting: Name: Advisory Committee for International Science and Engineering ( 25104). Date/Time... Engineering, Reports from Advisory Committee Working Groups. April 26, 2011 AM: Globalization of Universities...

  9. 77 FR 57085 - Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... COMMISSION Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital... Communications Commission's (FCC) Advisory Committee on Diversity for Communications in the Digital Age (``Diversity Committee''). The Committee's mission is to provide recommendations to the Commission...

  10. 76 FR 64348 - Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... COMMISSION Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital... Communications Commission's (FCC) Advisory Committee on Diversity for Communications in the Digital Age (``Diversity Committee''). The Committee's mission is to provide recommendations to the Commission...

  11. 78 FR 21354 - Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-10

    ... COMMISSION Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital... Communications Commission's (FCC) Advisory Committee on Diversity for Communications in the Digital Age (``Diversity Committee''). The Committee's mission is to provide recommendations to the Commission...

  12. 77 FR 6113 - Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-07

    ... COMMISSION Federal Advisory Committee Act; Advisory Committee on Diversity for Communications in the Digital... Communications Commission's (FCC's) Advisory Committee on Diversity for Communications in the Digital Age (``Diversity Committee''). The Committee's mission is to provide recommendations to the Commission...

  13. Mitochondrial membrane potential is reduced in copper-deficient C2C12 cells in the absence of apoptosis.

    PubMed

    Chen, Xiulian; Medeiros, Denis M; Jennings, Dianne

    2005-07-01

    Mitochondrial membrane potential is reduced in copper-deficient rat hearts, but it is uncertain if this will lead to the onset of apoptosis. To determine if copper deficiency per se leads to apoptosis, C2C12 cells were made copper deficient by treatment with the copper chelator tetraethylenepentamine (TEPA). In TEPA-treated cells, the activity of Cu, Zn-superoxide dismutase and cytochrome-c oxidase decreased dramatically. The protein levels of nuclear-encoded subunits of the cytochromie-c oxidase decreased, but the mitochondrial-encoded subunits remained unchanged. Decreased mitochondrial membrane potential was indicated in TEPA-treated cells, but further investigation of the potential induction of apoptosis by measuring caspase-3 activity, protein concentrations of Bcl-2 and Bax, and DNA fragmentation suggested that apoptosis is not induced in TEPA-treated C2C12 cells. Cells with decreased mitochondrial membrane potential were not destined to apoptosis as a result of copper deficiency.

  14. Mass spectrometric 13C/12C determinations to detect high fructose corn syrup in orange juice: collaborative study.

    PubMed

    Doner, L W; Bills, D D; Carro, O; Drimmie, R; Fritz, P; Gearing, J N; Hillaire-Marcel, C; Parker, P L; Reeseman, F M; Smith, B N; Ziegler, H

    1982-05-01

    The 13C/12C ratios in orange juice are sufficiently uniform and different from those in high fructose corn syrup (HFCS) so that the addition of HFCS to orange juice can be detected. HFCS averages -9.7% (parts per thousand) delta 13C, orange juice averages -24.5%, and mixtures of HFCS and orange juice possess intermediate values. One pure orange juice and 4 orange juice -HFCS mixtures containing from 25 to 70% orange juice were properly classified by 7 collaborators. Samples with delta 13C values less negative than -22.1%, 4 standard deviations from the mean of pure juices, can, with a high degree of confidence, be classified as adulterated. Samples with values more negative than -22.1% must be considered unadulterated with HFCS, because pure orange juices possess a range of delta 13C values. The 13C/12C mass spectrometric method was adopted official first action for detecting HFCS in orange juice.

  15. 78 FR 69991 - Advisory Committee; Veterinary Medicine Advisory Committee; Termination

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-22

    ... HUMAN SERVICES Food and Drug Administration 21 CFR Part 14 Advisory Committee; Veterinary Medicine... Food and Drug Administration (FDA) is announcing the termination of the Veterinary Medicine Advisory Committee. This document removes the Veterinary Advisory Committee from the Agency's list of...

  16. 77 FR 21619 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-10

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will... implementation of new measures; Role of the human element; Formal safety assessment; Piracy and armed...

  17. 78 FR 17467 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... seafarers in the event of a maritime accident. --Piracy. --Collation and preservation of evidence...

  18. 77 FR 12353 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-29

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... Convention, 2006; Fair treatment of seafarers in the event of a maritime accident; Piracy; Matters...

  19. 76 FR 12787 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... piracy; --Matters arising from the 105th regular session of the IMO Council; --Technical...

  20. 75 FR 63888 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... regarding piracy --Matters arising from the 25th extraordinary and the 104th regular sessions of the...

  1. 78 FR 77773 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... found at: www.uscg.mil/imo . Dated: December 17, 2013. Marc Zlomek, Executive Secretary, Shipping...

  2. 78 FR 6399 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an... www.uscg.mil/hq/cg5/cg523/imo . Dated: January 22, 2013. Brian Robinson, Executive Secretary, Shipping...

  3. 75 FR 17822 - Shipping Coordinating Committee; Notice of Committee Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-07

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF STATE Shipping Coordinating Committee; Notice of Committee Meeting The Shipping Coordinating Committee (SHC) will conduct an open meeting at 10 a.m. on Tuesday, April 20, 2010, in Room 1422 of the United States Coast Guard...

  4. Response of C2C12 myoblasts to hypoxia: the relative roles of glucose and oxygen in adaptive cellular metabolism.

    PubMed

    Li, Wei; Hu, Zhen-Fu; Chen, Bin; Ni, Guo-Xin

    2013-01-01

    Oxygen and glucose are two important nutrients for mammalian cell function. In this study, the effect of glucose and oxygen concentrations on C2C12 cellular metabolism was characterized with an emphasis on detecting whether cells show oxygen conformance (OC) in response to hypoxia. After C2C12 cells being cultured in the levels of glucose at 0.6 mM (LG), 5.6 mM (MG), or 23.3 mM(HG) under normoxic or hypoxic (1% oxygen) condition, cellular oxygen consumption, glucose consumption, lactate production, and metabolic status were determined. Short-term oxygen consumption was measured with a novel oxygen biosensor technique. Longer-term measurements were performed with standard glucose, lactate, and cell metabolism assays. It was found that oxygen depletion in normoxia is dependent on the glucose concentration in the medium. Cellular glucose uptake and lactate production increased significantly in hypoxia than those in normoxia. In hypoxia the cellular response to the level of glucose was different to that in normoxia. The metabolic activities decreased while glucose concentration increased in normoxia, while in hypoxia, metabolic activity was reduced in LG and MG, but unchanged in HG condition. The OC phenomenon was not observed in the present study. Our findings suggested that a combination of low oxygen and low glucose damages the viability of C2C12 cells more seriously than low oxygen alone. In addition, when there is sufficient glucose, C2C12 cells will respond to hypoxia by upregulating anaerobic respiration, as shown by lactate production.

  5. Nandrolone, an anabolic steroid, stabilizes Numb protein through inhibition of mdm2 in C2C12 myoblasts.

    PubMed

    Liu, Xin-Hua; Yao, Shen; Levine, Alice C; Kirschenbaum, Alexander; Pan, Jiangping; Wu, Yong; Qin, Weiping; Collier, Lauren; Bauman, William A; Cardozo, Christopher P

    2012-01-01

    Nandrolone, an anabolic steroid, slows denervation atrophy of rat muscle, prevents denervation-induced nuclear accumulation of intracellular domain of the Notch receptor, and elevates expression of Numb. Numb acts as an inhibitor of Notch signaling and promotes myogenic differentiation of satellite cells. Turnover of Numb is regulated by mdm2, an E3 ubiquitin ligase. With these considerations in mind, we investigated the effects of nandrolone on the expression of Numb and mdm2 proteins and determined the effect of mdm2 on nandrolone-induced alterations in Numb protein in C2C12 myoblasts. When C2C12 cells were cultured in a medium favoring differentiation (Dulbecco modified Eagle medium containing 2% horse serum), nandrolone up-regulated Numb protein levels in a time-dependent manner and prolonged Numb protein half-life from 10 to 18 hours. In contrast, nandrolone reduced the expression of mdm2 protein. To determine whether the decreased mdm2 expression induced by nandrolone was responsible for the increased levels and prolonged half-life of Numb protein in this cell line, mdm2-small interfering RNA (siRNA) was employed to inhibit mdm2 expression. Compared to cells transfected with scrambled siRNA (negative control), transfection with mdm2-siRNA increased basal Numb protein expression but abolished the further increase in Numb protein levels by nandrolone. In addition, transfection of mdm2-siRNA mimicked the effect of nandrolone to prolong the half-life of Numb protein. Moreover, when C2C12 cells were forced to overexpress mdm2, there was a significant decline in the expression of both basal and inducible Numb protein. Our data suggest that nandrolone, by a novel mechanism for this agent in a muscle cell type, increases Numb protein levels in C2C12 myoblasts by stabilizing Numb protein against degradation, at least in part, via suppression of mdm2 expression.

  6. Resistin Increases Ectopic Deposition of Lipids Through miR-696 in C2C12 Cells.

    PubMed

    Wen, Fengyun; Zhang, Haiwei; Bao, Chen; Yang, Mengshi; Wang, Nan; Zhang, Jie; Hu, Yajie; Yang, Xi; Geng, Jinjing; Yang, Zaiqing

    2015-06-01

    Resistin is associated with ectopic deposition of lipids, and determining its developmental and molecular mechanisms may help in the development of novel treatments. MicroRNAs (miRNAs) are involved in many physiological and pathological processes as negative regulators. We performed mouse liver miRNA microarrays to analyze the differences in expression between resistin-treated and control mice; the results showed that miR-696 was significantly upregulated by resistin. Therefore, we aimed to study whether miR-696 played a role in the resistin-induced ectopic deposition of lipids. Quantitative RT-PCR results showed that miR-696 was upregulated both in vivo and in vitro, consistent with the microarray. We transfected C2C12 cells and used miR-696 mimics and inhibitors to assess the role of miR-696 in the resistin-induced ectopic deposition of lipids. The overexpression of miR-696 increased the TG content in C2C12 cells and decreased the mitochondrial content. Next, a study of miR-696's role in the deposition of lipids in C2C12 induced by resistin showed that inhibition of miR-696 restored the TG content by up to 80%, which suggests that, in C2C12 cells, resistin at least partially increases the deposition of lipids through miR-696. miR-696 plays a role in the ectopic deposition of lipids induced by resistin in skeletal muscle at least partially.

  7. G-CSF does not influence C2C12 myogenesis despite receptor expression in healthy and dystrophic skeletal muscle

    PubMed Central

    Wright, Craig R.; Brown, Erin L.; Della-Gatta, Paul A.; Ward, Alister C.; Lynch, Gordon S.; Russell, Aaron P.

    2014-01-01

    Granulocyte-colony stimulating factor (G-CSF) increases recovery of rodent skeletal muscles after injury, and increases muscle function in rodent models of neuromuscular disease. However, the mechanisms by which G-CSF mediates these effects are poorly understood. G-CSF acts by binding to the membrane spanning G-CSFR and activating multiple intracellular signaling pathways. Expression of the G-CSFR within the haematopoietic system is well known, but more recently it has been demonstrated to be expressed in other tissues. However, comprehensive characterization of G-CSFR expression in healthy and diseased skeletal muscle, imperative before implementing G-CSF as a therapeutic agent for skeletal muscle conditions, has been lacking. Here we show that the G-CSFR is expressed in proliferating C2C12 myoblasts, differentiated C2C12 myotubes, human primary skeletal muscle cell cultures and in mouse and human skeletal muscle. In mdx mice, a model of human Duchenne muscular dystrophy (DMD), G-CSF mRNA and protein was down-regulated in limb and diaphragm muscle, but circulating G-CSF ligand levels were elevated. G-CSFR mRNA in the muscles of mdx mice was up-regulated however steady-state levels of the protein were down-regulated. We show that G-CSF does not influence C2C12 myoblast proliferation, differentiation or phosphorylation of Akt, STAT3, and Erk1/2. Media change alone was sufficient to elicit increases in Akt, STAT3, and Erk1/2 phosphorylation in C2C12 muscle cells and suggest previous observations showing a G-CSF increase in phosphoprotein signaling be viewed with caution. These results suggest that the actions of G-CSF may require the interaction with other cytokines and growth factors in vivo, however these data provides preliminary evidence supporting the investigation of G-CSF for the management of muscular dystrophy. PMID:24822049

  8. Quantum partner-dance in the 12C + 12C system yields sub-Coulomb fusion resonances

    NASA Astrophysics Data System (ADS)

    Diaz-Torres, Alexis; Wiescher, Michael

    2014-03-01

    A preliminary study of the 12C + 12C sub-Coulomb fusion reaction using the time-dependent wave-packet method is presented. The theoretical sub-Coulomb fusion resonances seem to correspond well with observations. The present method might be a more suitable tool for expanding the cross-section predictions towards lower energies than the commonly used potential-model approximation.

  9. Linewidth measurements in the thermal infrared bands of (C-12)H3D at planetary atmospheric temperatures

    NASA Technical Reports Server (NTRS)

    Varanasi, Prasad; Chudamani, S.

    1989-01-01

    Collision-broadened halfwidths of three lines in the nu3 fundamental band and of four lines in the nu6 fundamental band of (C-12)H3D have been measured at temperatures between 123 and 295 K using the Doppler-limited spectral resolution of a tunable diode laser spectrometer. Temperature dependence of the linewidths has been determined in self-broadening and in broadening by H2, He and N2.

  10. MicroRNAs Regulate Cellular ATP Levels by Targeting Mitochondrial Energy Metabolism Genes during C2C12 Myoblast Differentiation.

    PubMed

    Siengdee, Puntita; Trakooljul, Nares; Murani, Eduard; Schwerin, Manfred; Wimmers, Klaus; Ponsuksili, Siriluck

    2015-01-01

    In our previous study, we identified an miRNA regulatory network involved in energy metabolism in porcine muscle. To better understand the involvement of miRNAs in cellular ATP production and energy metabolism, here we used C2C12 myoblasts, in which ATP levels increase during differentiation, to identify miRNAs modulating these processes. ATP level, miRNA and mRNA microarray expression profiles during C2C12 differentiation into myotubes were assessed. The results suggest 14 miRNAs (miR-423-3p, miR-17, miR-130b, miR-301a/b, miR-345, miR-15a, miR-16a, miR-128, miR-615, miR-1968, miR-1a/b, and miR-194) as cellular ATP regulators targeting genes involved in mitochondrial energy metabolism (Cox4i2, Cox6a2, Ndufb7, Ndufs4, Ndufs5, and Ndufv1) during C2C12 differentiation. Among these, miR-423-3p showed a high inverse correlation with increasing ATP levels. Besides having implications in promoting cell growth and cell cycle progression, its function in cellular ATP regulation is yet unknown. Therefore, miR-423-3p was selected and validated for the function together with its potential target, Cox6a2. Overexpression of miR-423-3p in C2C12 myogenic differentiation lead to decreased cellular ATP level and decreased expression of Cox6a2 compared to the negative control. These results suggest miR-423-3p as a novel regulator of ATP/energy metabolism by targeting Cox6a2.

  11. MicroRNAs Regulate Cellular ATP Levels by Targeting Mitochondrial Energy Metabolism Genes during C2C12 Myoblast Differentiation

    PubMed Central

    Siengdee, Puntita; Trakooljul, Nares; Murani, Eduard; Schwerin, Manfred; Wimmers, Klaus; Ponsuksili, Siriluck

    2015-01-01

    In our previous study, we identified an miRNA regulatory network involved in energy metabolism in porcine muscle. To better understand the involvement of miRNAs in cellular ATP production and energy metabolism, here we used C2C12 myoblasts, in which ATP levels increase during differentiation, to identify miRNAs modulating these processes. ATP level, miRNA and mRNA microarray expression profiles during C2C12 differentiation into myotubes were assessed. The results suggest 14 miRNAs (miR-423-3p, miR-17, miR-130b, miR-301a/b, miR-345, miR-15a, miR-16a, miR-128, miR-615, miR-1968, miR-1a/b, and miR-194) as cellular ATP regulators targeting genes involved in mitochondrial energy metabolism (Cox4i2, Cox6a2, Ndufb7, Ndufs4, Ndufs5, and Ndufv1) during C2C12 differentiation. Among these, miR-423-3p showed a high inverse correlation with increasing ATP levels. Besides having implications in promoting cell growth and cell cycle progression, its function in cellular ATP regulation is yet unknown. Therefore, miR-423-3p was selected and validated for the function together with its potential target, Cox6a2. Overexpression of miR-423-3p in C2C12 myogenic differentiation lead to decreased cellular ATP level and decreased expression of Cox6a2 compared to the negative control. These results suggest miR-423-3p as a novel regulator of ATP/energy metabolism by targeting Cox6a2. PMID:26010876

  12. Transparency in Canadian public drug advisory committees.

    PubMed

    Rosenberg-Yunger, Zahava R S; Bayoumi, Ahmed M

    2014-11-01

    Transparency in health care resource allocation decisions is a criterion of a fair process. We used qualitative methods to explore transparency across 11 Canadian drug advisory committees. We developed seven criteria to assess transparency (disclosure of members' names, disclosure of membership selection criteria, disclosure of conflict of interest guidelines and members' conflicts, public posting of decisions not to fund drugs, public posting of rationales for decisions, stakeholder input, and presence of an appeals mechanism) and two sub-criteria for when rationales were posted (direct website link and readability). We interviewed a purposeful sample of key informants who were conversant in English and a current or past member of either a committee or a stakeholder group. We analyzed data using a thematic approach. Interviewing continued until saturation was reached. We examined documents from 10 committees and conducted 27 interviews. The median number of criteria addressed by committees was 2 (range 0-6). Major interview themes included addressing: (1) accessibility issues, including stakeholders' degree of access to the decision making process and appeal mechanisms; (2) communication issues, including improving internal and external communication and public access to information; and (3) confidentiality issues, including the use of proprietary evidence. Most committees have some mechanisms to address transparency but none had a fully transparent process. The most important ways to improve transparency include creating formal appeal mechanisms, improving communication, and establishing consistent rules about the use of, and public access to, proprietary evidence. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  13. Individual CLA Isomers, c9t11 and t10c12, Prevent Excess Liver Glycogen Storage and Inhibit Lipogenic Genes Expression Induced by High-Fructose Diet in Rats.

    PubMed

    Maslak, Edyta; Buczek, Elzbieta; Szumny, Antoni; Szczepnski, Wojciech; Franczyk-Zarow, Magdalena; Kopec, Aneta; Chlopicki, Stefan; Leszczynska, Teresa; Kostogrys, Renata B

    2015-01-01

    This study assessed the effects of individual conjugated linoleic acid isomers, c9t11-CLA and t10c12-CLA, on nonalcoholic fatty liver disease (NAFLD) and systemic endothelial dysfunction in rats fed for four weeks with control or high-fructose diet. The high-fructose diet hampered body weight gain (without influencing food intake), increased liver weight and glycogen storage in hepatocytes, upregulated expression of fatty acid synthase (FAS) and stearoyl-CoA desaturase-1 (SCD-1), and increased saturated fatty acid (SFA) content in the liver. Both CLA isomers prevented excessive accumulation of glycogen in the liver. Specifically, t10c12-CLA decreased concentration of serum triacylglycerols and LDL + VLDL cholesterol, increased HDL cholesterol, and affected liver lipid content and fatty acid composition by downregulation of liver SCD-1 and FAS expression. In turn, the c9t11-CLA decreased LDL+VLDL cholesterol in the control group and downregulated liver expression of FAS without significant effects on liver weight, lipid content, and fatty acid composition. In summary, feeding rats with a high-fructose diet resulted in increased liver glycogen storage, indicating the induction of gluconeogenesis despite simultaneous upregulation of genes involved in de novo lipogenesis. Although both CLA isomers (c9t11 and t10c12) display hepatoprotective activity, the hypolipemic action of the t10c12-CLA isomer proved to be more pronounced than that of c9t11-CLA.

  14. Federal Advisory Committees at EPA

    EPA Pesticide Factsheets

    FACA committees are created to obtain advice on a wide range of environmental issues. Every committee meeting, no matter what the subject matter or where it is held, is dedicated to open government and citizen participation.

  15. Interagency Suspension and Debarment Committee

    EPA Pesticide Factsheets

    The Interagency Suspension and Debarment Committee (ISDC) was created, as an Office of Management and Budget (OMB) committee, by Executive Order 12549 for the purpose of monitoring the implementation of the Order.

  16. Pathogenicity and immunogenicity of recombinant Tiantan Vaccinia Virus with deleted C12L and A53R genes.

    PubMed

    Dai, Kaifan; Liu, Ying; Liu, Mingjie; Xu, Jianqing; Huang, Wei; Huang, Xianggang; Liu, Lianxing; Wan, Yanmin; Hao, Yanling; Shao, Yiming

    2008-09-15

    Interest is increasing regarding replicating poxvirus as HIV vaccine vector. In China, the Tiantan Vaccinia Virus (TV) has been used most extensively in the battle of eradicating smallpox. Recently, TV was developing as vaccine vector to fight against infectious diseases such as human immunodeficiency virus (HIV). However, replicating vaccinia virus sometimes may pose serious post-vaccination complications, especially in immunosuppressed individuals. To develop a safer and more effective TV-based vector, we constructed C12L (vIL-18 binding protein) and A53R (vTNF receptor homolog) gene-deleted mutants which are based on parental TV and VTKgpe (TV expressing HIV gagpol and env gene), respectively. The pathogenicity and immunogenicity were also evaluated. Deleting these two immunomodulatory genes lessened the virulence of the parental virus in both mice and rabbit models. Notably, C12L deletion mutant attenuated the skin virulence of parental virus by as high as approximate 2 logs. Furthermore, VTKgpe with A53R and C12L gene deletion retains the high immunogenicity of the parental virus to elicit strong humoral and cellular responses to the HIV target genes despite the remarkable attenuation. These data suggest that deletion of the cytokine viroceptor gene is feasible to obtain a safer and replication-competent TV vector for vaccination and immunotherapy.

  17. microRNA133a Targets Foxl2 and Promotes Differentiation of C2C12 into Myogenic Progenitor Cells

    PubMed Central

    Luo, Yueqiu; Wu, Xiaoxing; Ling, Zongxin; Yuan, Li; Cheng, Yiwen; Chen, Jingyang

    2015-01-01

    microRNAs are endogenous noncoding RNA molecules of ∼22 nucleotides that regulate gene function by modification of target mRNAs. Due to tissue specific of miR-133a and miR-1/206 for skeletal muscles, we investigated the role of miR-133a and miR-1/206 in promoting the differentiation of the C2C12 cells. The results show that directly transfecting mature miR-133a, miR-1/206, or combinations (miR-1 and miR-206, miR-1 and miR-133a, and miR-133a and miR-206) into C2C12 cells, respectively, for 5 days induces formation of myogenic progenitor cells. Overexpression of miR-133a and miR-206 in C2C12 cells greatly improved multinucleated myotube formation. microRNA-133a (miR-133a) is highly expressed during human muscle development. Using bioinformatics, we identified one putative miR-133a binding site within the 3′-untranslated region of the mouse Foxl2 mRNA. The expression of Foxl2 was shown to be downregulated by subsequent western blot analysis. PMID:25317675

  18. microRNA133a targets Foxl2 and promotes differentiation of C2C12 into myogenic progenitor cells.

    PubMed

    Luo, Yueqiu; Wu, Xiaoxing; Ling, Zongxin; Yuan, Li; Cheng, Yiwen; Chen, Jingyang; Xiang, Charlie

    2015-01-01

    microRNAs are endogenous noncoding RNA molecules of ∼22 nucleotides that regulate gene function by modification of target mRNAs. Due to tissue specific of miR-133a and miR-1/206 for skeletal muscles, we investigated the role of miR-133a and miR-1/206 in promoting the differentiation of the C2C12 cells. The results show that directly transfecting mature miR-133a, miR-1/206, or combinations (miR-1 and miR-206, miR-1 and miR-133a, and miR-133a and miR-206) into C2C12 cells, respectively, for 5 days induces formation of myogenic progenitor cells. Overexpression of miR-133a and miR-206 in C2C12 cells greatly improved multinucleated myotube formation. microRNA-133a (miR-133a) is highly expressed during human muscle development. Using bioinformatics, we identified one putative miR-133a binding site within the 3'-untranslated region of the mouse Foxl2 mRNA. The expression of Foxl2 was shown to be downregulated by subsequent western blot analysis.

  19. Determination of the (13)C/(12)C Carbon Isotope Ratio in Carbonates and Bicarbonates by (13)C NMR Spectroscopy.

    PubMed

    Pironti, Concetta; Cucciniello, Raffaele; Camin, Federica; Tonon, Agostino; Motta, Oriana; Proto, Antonio

    2017-10-09

    This paper is the first study focused on the innovative application of (13)C NMR (nuclear magnetic resonance) spectroscopy to determine the bulk (13)C/(12)C carbon isotope ratio, at natural abundance, in inorganic carbonates and bicarbonates. In the past, (13)C NMR spectroscopy (irm-(13)C NMR) was mainly used to measure isotope ratio monitoring with the potential of conducting (13)C position-specific isotope analysis of organic molecules with high precision. The reliability of the newly developed methodology for the determination of stable carbon isotope ratio was evaluated in comparison with the method chosen in the past for these measurements, i.e., isotope ratio mass spectrometry (IRMS), with very encouraging results. We determined the (13)C/(12)C ratio of carbonates and bicarbonates (∼50-100 mg) with a precision on the order of 1‰ in the presence of a relaxation agent, such as Cr(acac)3, and CH3(13)COONa as an internal standard. The method was first applied to soluble inorganic carbonates and bicarbonates and then extended to insoluble carbonates by converting them to Na2CO3, following a simple procedure and without observing isotopic fractionation. Here, we demonstrate that (13)C NMR spectroscopy can also be successfully adopted to characterize the (13)C/(12)C isotope ratio in inorganic carbonates and bicarbonates with applications in different fields, such as cultural heritage and geological studies.

  20. Selective androgen receptor modulator, YK11, regulates myogenic differentiation of C2C12 myoblasts by follistatin expression.

    PubMed

    Kanno, Yuichiro; Ota, Rumi; Someya, Kousuke; Kusakabe, Taichi; Kato, Keisuke; Inouye, Yoshio

    2013-01-01

    The myogenic differentiation of C2C12 myoblast cells is induced by the novel androgen receptor (AR) partial agonist, (17α,20E)-17,20-[(1-methoxyethylidene)bis-(oxy)]-3-oxo-19-norpregna-4,20-diene-21-carboxylic acid methyl ester (YK11), as well as by dihydrotestosterone (DHT). YK11 is a selective androgen receptor modulator (SARM), which activates AR without the N/C interaction. In this study, we further investigated the mechanism by which YK11 induces myogenic differentiation of C2C12 cells. The induction of key myogenic regulatory factors (MRFs), such as myogenic differentiation factor (MyoD), myogenic factor 5 (Myf5) and myogenin, was more significant in the presence of YK11 than in the presence of DHT. YK11 treatment of C2C12 cells, but not DHT, induced the expression of follistatin (Fst), and the YK11-mediated myogenic differentiation was reversed by anti-Fst antibody. These results suggest that the induction of Fst is important for the anabolic effect of YK11.