A Case Study of Coordination in Distributed Agile Software Development

NASA Astrophysics Data System (ADS)

Hole, Steinar; Moe, Nils Brede

Global Software Development (GSD) has gained significant popularity as an emerging paradigm. Companies also show interest in applying agile approaches in distributed development to combine the advantages of both approaches. However, in their most radical forms, agile and GSD can be placed in each end of a plan-based/agile spectrum because of how work is coordinated. We describe how three GSD projects applying agile methods coordinate their work. We found that trust is needed to reduce the need of standardization and direct supervision when coordinating work in a GSD project, and that electronic chatting supports mutual adjustment. Further, co-location and modularization mitigates communication problems, enables agility in at least part of a GSD project, and renders the implementation of Scrum of Scrums possible.

Ultraviolet (UV)-reflective paint with ultraviolet germicidal irradiation (UVGI) improves decontamination of nosocomial bacteria on hospital room surfaces.

PubMed

Jelden, Katelyn C; Gibbs, Shawn G; Smith, Philip W; Hewlett, Angela L; Iwen, Peter C; Schmid, Kendra K; Lowe, John J

2017-06-01

An ultraviolet germicidal irradiation (UVGI) generator (the TORCH, ClorDiSys Solutions, Inc.) was used to compare the disinfection of surface coupons (plastic from a bedrail, stainless steel, and chrome-plated light switch cover) in a hospital room with walls coated with ultraviolet (UV)-reflective paint (Lumacept) or standard paint. Each surface coupon was inoculated with methicillin-resistant Staphylococcus aureus (MRSA) or vancomycin-resistant Enterococcus faecalis (VRE), placed at 6 different sites within a hospital room coated with UV-reflective paint or standard paint, and treated by 10 min UVC exposure (UVC dose of 0-688 mJ/cm 2 between sites with standard paint and 0-553 mJ/cm 2 with UV-reflective paint) in 8 total trials. Aggregated MRSA concentrations on plastic bedrail surface coupons were reduced on average by 3.0 log 10 (1.8 log 10 Geometric Standard Deviation [GSD]) with standard paint and 4.3 log 10 (1.3 log 10 GSD) with UV-reflective paint (p = 0.0005) with no significant reduction differences between paints on stainless steel and chrome. Average VRE concentrations were reduced by ≥4.9 log 10 (<1.2 log 10 GSD) on all surface types with UV-reflective paint and ≤4.1 log 10 (<1.7 log 10 GSD) with standard paint (p < 0.05). At 5 aggregated sites directly exposed to UVC light, MRSA concentrations on average were reduced by 5.2 log 10 (1.4 log 10 GSD) with standard paint and 5.1 log 10 (1.2 log 10 GSD) with UV-reflective paint (p = 0.017) and VRE by 4.4 log 10 (1.4 log 10 GSD) with standard paint and 5.3 log 10 (1.1 log 10 GSD) with UV-reflective paint (p < 0.0001). At one indirectly exposed site on the opposite side of the hospital bed from the UVGI generator, MRSA concentrations on average were reduced by 1.3 log 10 (1.7 log 10 GSD) with standard paint and 4.7 log 10 (1.3 log 10 GSD) with UV-reflective paint (p < 0.0001) and VRE by 1.2 log 10 (1.5 log 10 GSD) with standard paint and 4.6 log 10 (1.1 log 10 GSD) with UV-reflective paint (p < 0.0001). Coating hospital room walls with UV-reflective paint enhanced UVGI disinfection of nosocomial bacteria on various surfaces compared to standard paint, particularly at a surface placement site indirectly exposed to UVC light.

Generation rates and emission factors of particulate matter and particle-bound polycyclic aromatic hydrocarbons of incense sticks.

PubMed

Lung, Shih-Chun Candice; Hu, Shu-Chuan

2003-02-01

The generation rates and emission factors of particulate matter and associated polycyclic aromatic hydrocarbons (PAHs) from incense burning were assessed in a laboratory setting. The differences among different segments of the same stick, among different sticks of the same kind of incense, and between two kinds of manually made Chih-Chen incense sticks (A and B) were evaluated. Joss sticks were burned inside a 44 cm long elutriator; personal environmental monitors fitted into the top of the elutriator were used to take PM2.5 and PM10 samples of incense smoke. Samples were analyzed for PAHs by gas chromatography-flame ionization Detector. It was found that particle and associated PAHs were generated approximately at 561 microg/min (geometric standard deviation (GSD) = 1.1) and 0.56 microg/min (GSD = 1.1) from Incense A, and at 661 microg/min (GSD = 1.7) and 0.46 microg/min (GSD = 1.3) from Incense B, respectively. One gram of Incense A emitted about 19.8 mg (GSD = 1.1) particulate matter and 17.1 microg (GSD = 1.2) particulate-phase PAHs, while one gram of Incense B produced around 43.6 mg (GSD = 1.1) of particles and 25.2 microg (GSD = 1.2) of particle-bound PAHs. There were significant differences in emissions between Incenses A and B, although they belong to the same class of incense. A 10-20% variability in emissions was observed in the main part of the manually produced stick, and a larger variation was found at both tips of the combustible part.

Association of green stem disorder with agronomic traits in soybean

USDA-ARS?s Scientific Manuscript database

Green stem disorder (GSD) of soybean is the occurrence of non-senescent, fleshy green stems of plants with normal, fully mature pods and seeds. The main focus of this study was to determine the relationship between GSD incidence and agronomic traits and to determine if GSD incidence was associated w...

The Occurrence and Prevalence of Giraffe Skin Disease in Protected Areas of Northern Tanzania.

PubMed

Lee, Derek E; Bond, Monica L

2016-07-01

Giraffe skin disease (GSD) is a disorder of undetermined etiology that causes lesions on the forelimbs of Masai giraffe ( Giraffa camelopardalis tippelskirchi). We estimated occurrence and prevalence of GSD in six wildlife conservation areas of Tanzania. The disjunct spatial pattern of occurrence implies that environmental factors may influence GSD.

Microstructure as a function of the grain size distribution for packings of frictionless disks: Effects of the size span and the shape of the distribution.

PubMed

Estrada, Nicolas; Oquendo, W F

2017-10-01

This article presents a numerical study of the effects of grain size distribution (GSD) on the microstructure of two-dimensional packings of frictionless disks. The GSD is described by a power law with two parameters controlling the size span and the shape of the distribution. First, several samples are built for each combination of these parameters. Then, by means of contact dynamics simulations, the samples are densified in oedometric conditions and sheared in a simple shear configuration. The microstructure is analyzed in terms of packing fraction, local ordering, connectivity, and force transmission properties. It is shown that the microstructure is notoriously affected by both the size span and the shape of the GSD. These findings confirm recent observations regarding the size span of the GSD and extend previous works by describing the effects of the GSD shape. Specifically, we find that if the GSD shape is varied by increasing the proportion of small grains by a certain amount, it is possible to increase the packing fraction, increase coordination, and decrease the proportion of floating particles. Thus, by carefully controlling the GSD shape, it is possible to obtain systems that are denser and better connected, probably increasing the system's robustness and optimizing important strength properties such as stiffness, cohesion, and fragmentation susceptibility.

On the modified grain-size-distribution method to evaluate the dynamic recrystallisation fraction in AISI 304 stainless steel

NASA Astrophysics Data System (ADS)

Hong, D. H.; Park, J. K.

2018-04-01

The purpose of the present work was to verify the grain size distribution (GSD) method, which was recently proposed by one of the present authors as a method for evaluating the fraction of dynamic recrystallisation (DRX) in a microalloyed medium carbon steel. To verify the GSD-method, we have selected a 304 stainless steel as a model system and have measured the evolution of the overall grain size distribution (including both the recrystallised and unrecrystallised grains) during hot compression at 1,000 °C in a Gleeble machine; the DRX fraction estimated using the GSD method is compared with the experimentally measured value via EBSD. The results show that the previous GSD method tends to overestimate the DRX fraction due to the utilisation of a plain lognormal distribution function (LDF). To overcome this shortcoming, we propose a modified GSD-method wherein an area-weighted LDF, in place of a plain LDF, is employed to model the evolution of GSD during hot deformation. Direct measurement of the DRX fraction using EBSD confirms that the modified GSD-method provides a reliable method for evaluating the DRX fraction from the experimentally measured GSDs. Reasonable agreement between the DRX fraction and softening fraction suggests that the Kocks-Mecking method utilising the Voce equation can be satisfactorily used to model the work hardening and dynamic recovery behaviour of steels during hot deformation.

Myasthenia gravis in a patient affected by glycogen storage disease type Ib: a further manifestation of an increased risk for autoimmune disorders?

PubMed

Melis, D; Balivo, F; Della Casa, R; Romano, A; Taurisano, R; Capaldo, B; Riccardi, G; Monsurrò, M R; Parenti, G; Andria, G

2008-12-01

Glycogen storage disease type Ib (GSD Ib, OMIM 232220) is an inborn disorder of glucose metabolism, caused by mutations in the G6PT gene, encoding a glucose 6-phosphate transporter (G6PT). GSD Ib is mainly associated with fasting hypoglycaemia and hepatomegaly. Most GSD Ib patients also show neutropenia and neutrophil dysfunction and therefore are at risk of developing severe infections and inflammatory bowel disease (IBD). An increased risk for autoimmune disorders, such as thyroid autoimmunity and Crohn-like disease, has also been demonstrated, but no systematic study on the prevalence of autoimmune disorders in GSD Ib patients has ever been performed. We describe a 25-year-old patient affected by GSD Ib who developed 'seronegative' myasthenia gravis (MG), presenting with bilateral eyelid ptosis, diplopia, dysarthria, severe dysphagia, dyspnoea and fatigue. The repetitive stimulation of peripheral nerves test showed signs of exhaustion of neuromuscular transmission, particularly evident in the cranial area. Even in the absence of identifiable anti-acetylcholine receptor antibodies, seronegative MG is considered an autoimmune disorder and may be related to the disturbed immune function observed in GSD Ib patients.

Glycogen storage disease type 1: impact of medium-chain triglycerides on metabolic control and growth.

PubMed

Das, Anibh M; Lücke, Thomas; Meyer, Uta; Hartmann, Hans; Illsinger, Sabine

2010-01-01

Hypoketotic hypoglycaemia and hypertriglyceridaemia are biochemical hallmarks of glycogen storage disease (GSD) 1. Increased malonyl coenzyme A production which compromises oxidation of long-chain fatty acids via carnitine palmitoyltransferase (CPT) 1 inhibition plays a crucial role in the pathogenesis of these complications. Therapy consists primarily of nutritional support including frequent carbohydrate-rich meals. We studied the effect of a diet enriched in medium-chain triglycerides (MCT) on metabolic control/growth in GSD 1 as medium-chain fatty acids can be oxidised independently of CPT 1. An adult female, a 1.6-year-old boy with GSD 1a and a 6.5-year-old girl with GSD 1b treated with a classical GSD diet were enrolled; their 'classical GSD diet' was supplemented with MCT fats. Concentrations of glucose, lactate, ketone bodies triglycerides, uric acid, acylcarnitines in blood and organic acids in urine were determined. No clinical or biochemical side-effects were observed. The MCT diet led to a decrease in uric acid concentrations in all patients. Triglyceride levels were reduced only in the youngest patient, while lactate concentrations did not significantly decrease. The MCT diet allowed for a reduction in carbohydrate and caloric intake required to maintain euglycaemia and led to improvement in growth in the two prepubertal patients. MCT supplementation had a positive effect on metabolic control and growth in our patients suffering from GSD 1. (c) 2010 S. Karger AG, Basel.

Analysis of NOD1, NOD2, TLR1, TLR2, TLR4, TLR5, TLR6 and TLR9 genes in anal furunculosis of German shepherd dogs.

PubMed

House, A K; Binns, M M; Gregory, S P; Catchpole, B

2009-03-01

Anal furunculosis (AF) primarily affects German shepherd dogs (GSD) and is characterised by inflammation and ulceration of the perianal tissues with development of cutaneous sinuses or rectocutaneous fistulae. Investigation of pattern recognition receptor (PRR) function has suggested that defective responses might occur in AF-affected GSD. The aim of the current study was to investigate whether canine PRR genes are involved in determining susceptibility to AF in this breed. Chromosomal location and coding sequences for NOD1, NOD2, TLR1, TLR2, TLR4, TLR5, TLR6 and TLR9 were determined and microsatellite markers identified for each gene. Microsatellite genotyping of 100 control GSD and 47 AF-affected GSD showed restricted allelic variation for AHT H91 (associated with TLR5) and REN216 NO5 (associated with both TLR1 and TLR6) compared with non-GSD dogs. Genotyping of single nucleotide polymorphisms identified in canine TLR1, TLR5, TLR6 and NOD2 genes failed to show any significant associations between PRR polymorphisms and AF. The highly restricted PRR genotypes seen in GSD are likely to have resulted from selective breeding and might influence innate immune responses in this breed.

Translating person-centered care into practice: A comparative analysis of motivational interviewing, illness-integration support, and guided self-determination.

PubMed

Zoffmann, Vibeke; Hörnsten, Åsa; Storbækken, Solveig; Graue, Marit; Rasmussen, Bodil; Wahl, Astrid; Kirkevold, Marit

2016-03-01

Person-centred care [PCC] can engage people in living well with a chronic condition. However, translating PCC into practice is challenging. We aimed to compare the translational potentials of three approaches: motivational interviewing [MI], illness integration support [IIS] and guided self-determination [GSD]. Comparative analysis included eight components: (1) philosophical origin; (2) development in original clinical setting; (3) theoretical underpinnings; (4) overarching goal and supportive processes; (5) general principles, strategies or tools for engaging peoples; (6) health care professionals' background and training; (7) fidelity assessment; (8) reported effects. Although all approaches promoted autonomous motivation, they differed in other ways. Their original settings explain why IIS and GSD strive for life-illness integration, whereas MI focuses on managing ambivalence. IIS and GSD were based on grounded theories, and MI was intuitively developed. All apply processes and strategies to advance professionals' communication skills and engagement; GSD includes context-specific reflection sheets. All offer training programs; MI and GSD include fidelity tools. Each approach has a primary application: MI, when ambivalence threatens positive change; IIS, when integrating newly diagnosed chronic conditions; and GSD, when problem solving is difficult, or deadlocked. Professionals must critically consider the context in their choice of approach. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Grain size controls on sediment supply from debris-mantled dryland hillslopes

NASA Astrophysics Data System (ADS)

Michaelides, K.

2011-12-01

Debris-mantled hillslopes are common in arid and semiarid environments where low rates of chemical weathering give rise to thin, non-cohesive soils mantled with a layer of coarse rock fragments derived from weathered bedrock that can reach boulder size. The grain size distributions (GSDs) on the surface of these hillslopes interact with different magnitudes and frequencies of runoff-producing rainfall events that selectively transport grain sizes of different classes depending on flow, grain position on the slope, and hillslope attributes. Sediment transport over many runoff events determines sediment delivery to the slope base, which ultimately modifies the GSD of valley floors. The relationship between hillslope attributes and sediment flux forms the basis of geomorphic transport laws used to model the topographic evolution of drainage basins over >104 y timescales, but the specific responses of sediment flux across the hillslope and the corresponding changes in GSDs to individual storm events are poorly understood. Sheetwash erosion of coarse fragments presents a particular set of conditions for sediment transport that is poorly resolved in current models. A particle-based model for sheetwash sediment transport on debris-mantled hillslopes was developed within a rainfall-runoff model. The rainfall-runoff model produces spatial values of flow depth and velocity which are used to drive a particle-by-particle force-balance model derived from first principles for grain sizes > 1 mm. Particles on the hillslope surface are represented explicitly and can be composed of mixed grain sizes of any distribution or of uniform sizes of any diameter. The model resolves all the forces on each particle at each time and space step based on the flow hydraulics acting on them, so no assumptions are made about incipient motion using Shield's criterion. This research examines how the interplay between hillslope GSD, hillslope attributes (gradient and length) and runoff characteristics, determine sediment transport dynamics and net flux, GSD supplied to the slope base and the changes in GSD on the hillslope. The results show a strong control of initial hillslope GSD on flux characteristics: (1) GSD controls the degree of non-linearity in the relationship between sediment flux and hillslope gradient. (2) Grain size uniformity controls the degree and form of non-linearity in the relationship between sediment flux and gradient. (3) Over multiple runoff events, slopes coarsen - steeper slopes become coarser than shallow slopes. For individual events, changes in GSD on the slope depend on the magnitude and duration of the runoff event and can result in variable coarsening and fining on different parts of the slope. (4) The GSD of sediment delivered to the slope base is dependent on the hillslope GSD and the hillslope attributes and runoff characteristics. For most runoff events, the GSD of fluxed sediment is finer than the hillslope GSD except for extreme runoff events on very steep slopes with intermediate GSD (not extremely coarse). These findings provide insights into hillslope responses to climatic forcing and have theoretical implications for modeling hillslope evolution in drylands.

Effect of berberine on the viability of adipose tissue-derived mesenchymal stem cells in nutrients deficient condition.

PubMed

Ghorbani, Ahmad; Baradaran Rahimi, Vafa; Sadeghnia, Hamid Reza; Hosseini, Azar

2018-03-01

This study was designed to examine whether berberine protects rat adipose tissue-derived stem cells (ASCs) against glucose and serum deprivation (GSD)-induced cell death. ASCs were cultured for 24 h in GSD condition in the presence of berberine and then cell viability, apoptosis and generation of reactive oxygen species (ROS) were evaluated. The GSD condition significantly decreased ASCs viability and increased ROS generation and apoptosis. Incubation with 0.75-3 μM berberine partially increased cell viability and decreased ROS generation and apoptosis in GSD condition. In conclusion, berberine partially protects ASCs in nutrients deficient condition and may help ASCs to preserve their survival during cell therapy of ischemia.

Flexible guided self-determination intervention for younger adults with poorly controlled Type 1 diabetes, decreased HbA1c and psychosocial distress in women but not in men: a real-life RCT.

PubMed

Zoffmann, V; Vistisen, D; Due-Christensen, M

2015-09-01

To report results from an 18-month randomized controlled trial (RCT) testing the effectiveness of a flexible guided self-determination (GSD) intervention on glycaemic control and psychosocial distress in younger adults with poorly controlled Type 1 diabetes. Between January 2010 and February 2012, we randomly allocated two hundred 18-35-year-olds [mean age 25.7 (5.1) years, 50% men] with Type 1 diabetes for ≥ 1 year [mean duration 13.7 (6.8) years] and HbA1c  ≥ 64 mmol/mol (8.0%) to either an immediate GSD (intervention; n = 134) or 18-months delayed GSD group (control; n = 66). Group-based or individual GSD sessions were offered, drawing on reflection sheets and advanced professional communication. The primary outcome was HbA1c (measured at baseline and every three months thereafter) and among secondary outcomes was psychosocial distress (self-reported at baseline and after nine and 18 months). Intention-to-treat analyses included linear regression and repeated measurement analyses. A borderline significant decrease in HbA1c in the intervention group compared with the control group ( - 4 vs - 1 mmol/mol or - 0.4% vs - 0.1%; P = 0.073) was driven by a significantly greater reduction in the GSD women ( - 5 vs + 1 mmol/mol or - 0.5% vs + 0.1%; P = 0.017); parallel decreases were observed in the GSD and control men ( - 3 mmol/mol or  - 0.3%; P = 0.955). Significantly greater reduction in the GSD group's psychosocial distress was again driven by differences between the GSD and the control women. The men's improvements were not connected with the intervention. The flexible GSD intervention benefitted younger adult women by significantly improving glycaemic control and decreasing diabetes related distress. No effect was seen among men. © 2015 The Authors. Diabetic Medicine © 2015 Diabetes UK.

Sex determination, longevity, and the birth and death of reptilian species.

PubMed

Sabath, Niv; Itescu, Yuval; Feldman, Anat; Meiri, Shai; Mayrose, Itay; Valenzuela, Nicole

2016-08-01

Vertebrate sex-determining mechanisms (SDMs) are triggered by the genotype (GSD), by temperature (TSD), or occasionally, by both. The causes and consequences of SDM diversity remain enigmatic. Theory predicts SDM effects on species diversification, and life-span effects on SDM evolutionary turnover. Yet, evidence is conflicting in clades with labile SDMs, such as reptiles. Here, we investigate whether SDM is associated with diversification in turtles and lizards, and whether alterative factors, such as lifespan's effect on transition rates, could explain the relative prevalence of SDMs in turtles and lizards (including and excluding snakes). We assembled a comprehensive dataset of SDM states for squamates and turtles and leveraged large phylogenies for these two groups. We found no evidence that SDMs affect turtle, squamate, or lizard diversification. However, SDM transition rates differ between groups. In lizards TSD-to-GSD surpass GSD-to-TSD transitions, explaining the predominance of GSD lizards in nature. SDM transitions are fewer in turtles and the rates are similar to each other (TSD-to-GSD equals GSD-to-TSD), which, coupled with TSD ancestry, could explain TSD's predominance in turtles. These contrasting patterns can be explained by differences in life history. Namely, our data support the notion that in general, shorter lizard lifespan renders TSD detrimental favoring GSD evolution in squamates, whereas turtle longevity permits TSD retention. Thus, based on the macro-evolutionary evidence we uncovered, we hypothesize that turtles and lizards followed different evolutionary trajectories with respect to SDM, likely mediated by differences in lifespan. Combined, our findings revealed a complex evolutionary interplay between SDMs and life histories that warrants further research that should make use of expanded datasets on unexamined taxa to enable more conclusive analyses.

Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish.

PubMed

Zhu, Yao-Jun; Li, Xi-Yin; Zhang, Jun; Li, Zhi; Ding, Miao; Zhang, Xiao-Juan; Zhou, Li; Gui, Jian-Fang

2018-06-05

Coexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue. We found out that males of GSD and TSD in gibel carp had similar morphology, testicular histology, sperm structure and sperm vitality. However, when maternal individuals were mated with males of GSD, sperm nucleus swelling and fusing with the female pronucleus were observed in the fertilized eggs. On the contrary, when maternal individuals were mated with males of TSD, sperm nucleus remained in the condensed status throughout the whole process. Subsequently, semen proteomics analysis unveiled that DNA replication and gene expression-related pathways were inhibited in the sperm from males of TSD compared to males of GSD, and most differentially expressed proteins associated with DNA replication, transcription and translation were down-regulated. Moreover, via BrdU incorporation and immunofluorescence detection, male nucleus replication was revealed to be present in the fertilized eggs by the sperm from males of GSD, but absent in the fertilized eggs by the sperm from males of TSD. These findings indicate that DNA replication and gene expression-related pathways are associated with the distinct sperm nucleus development behaviors in fertilized eggs in response to the sperm from males of GSD and TSD. And this study is the first attempt to screen the differences between males determined via GSD and TSD in gynogenetic species, which might give a hint for understanding evolutionary adaption of diverse sex determination mechanisms in unisexual vertebrates.

DoGSD: the dog and wolf genome SNP database.

PubMed

Bai, Bing; Zhao, Wen-Ming; Tang, Bi-Xia; Wang, Yan-Qing; Wang, Lu; Zhang, Zhang; Yang, He-Chuan; Liu, Yan-Hu; Zhu, Jun-Wei; Irwin, David M; Wang, Guo-Dong; Zhang, Ya-Ping

2015-01-01

The rapid advancement of next-generation sequencing technology has generated a deluge of genomic data from domesticated dogs and their wild ancestor, grey wolves, which have simultaneously broadened our understanding of domestication and diseases that are shared by humans and dogs. To address the scarcity of single nucleotide polymorphism (SNP) data provided by authorized databases and to make SNP data more easily/friendly usable and available, we propose DoGSD (http://dogsd.big.ac.cn), the first canidae-specific database which focuses on whole genome SNP data from domesticated dogs and grey wolves. The DoGSD is a web-based, open-access resource comprising ∼ 19 million high-quality whole-genome SNPs. In addition to the dbSNP data set (build 139), DoGSD incorporates a comprehensive collection of SNPs from two newly sequenced samples (1 wolf and 1 dog) and collected SNPs from three latest dog/wolf genetic studies (7 wolves and 68 dogs), which were taken together for analysis with the population genetic statistics, Fst. In addition, DoGSD integrates some closely related information including SNP annotation, summary lists of SNPs located in genes, synonymous and non-synonymous SNPs, sampling location and breed information. All these features make DoGSD a useful resource for in-depth analysis in dog-/wolf-related studies. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

Polymorphism and Expression Profile of Cholecystokinin Type A Receptor in Relation to Gallstone Disease Susceptibility.

PubMed

Kazmi, Hasan Raza; Chandra, Abhijit; Nigam, Jaya; Baghel, Kavita; Srivastava, Meenu; Maurya, Shailendra S; Parmar, Devendra

2016-10-01

In the present study, we investigated expression pattern of Cholecystokinin type A receptor (CCKAR) in relation to its commonly studied polymorphism (rs1800857, T/C) in gallstone disease (GSD) patients and controls. A total of 502 subjects (272 GSD and 230 controls) were enrolled, and genotyping was performed by evaluating restriction fragments of PstI digested DNA. For analyzing expression pattern of CCKAR in relation to polymorphism, gallbladder tissue samples from 80 subjects (GSD-55; control-25) were studied. Expression of CCKAR mRNA was evaluated by reverse transcriptase-PCR and confirmed using real-time PCR. Protein expression was evaluated by enzyme-linked immunosorbent assay. We observed significantly (p < 0.0001) lower expression of CCKAR mRNA and protein in GSD tissues as compared with control. Significantly higher frequency of A1/A1 genotype (C/T transition) (p = 0.0005) was observed for GSD as compared with control. Expression of CCKAR protein was found to be significantly lower (p < 0.0001) in A1/A1 genotype as compared with other genotypes for GSD patients. Perhaps, this is the first report providing evidence of alteration in CCKAR expression in relation to its polymorphism elucidating the molecular pathway of the disease. Additional investigations with lager sample size are needed to confirm these findings.

Does the mechanism of sex determination constrain the potential for sex manipulation? A test in geckos with contrasting sex-determining systems

NASA Astrophysics Data System (ADS)

Kratochvíl, Lukáš; Kubička, Lukáš; Landová, Eva

2008-03-01

The concentration of yolk steroids was suggested to influence offspring gender in oviparous animals subject to both temperature-dependent sex determination (TSD) and genotypic sex determination (GSD). However, the proposed mechanisms of steroid effects are thought to differ between TSD and GSD: a direct effect of oestrogens on gonad feminisation in TSD species vs a differential induction of male-producing or female-producing gametes in GSD species. Geckos offer an ideal opportunity for testing these suggested mechanisms. Closely related gecko species differ in their modes of sex determination. They lay clutches of two synchronously formed eggs; both eggs share equal steroid levels. If identical hormonal composition and environment during vitellogenesis, gravidity and incubation determine the sex of the progeny, siblings should share the same gender in both TSD and GSD geckos. We found strong support for this prediction in a TSD gecko species. Among clutches that were incubated at the temperature that produced both sexes, there were no clutches with siblings of the opposite sex. On the other hand, about half of the clutches yielded siblings of the opposite sex in four GSD species. These results suggest that sex-determining systems constrain the ability of the female to produce single-sex siblings and, hence, it seems that the GSD mechanism constrains the opportunities for sex ratio manipulation in geckos via yolk steroid manipulation.

The gene for glycogen-storage disease type 1b maps to chromosome 11q23.

PubMed

Annabi, B; Hiraiwa, H; Mansfield, B C; Lei, K J; Ubagai, T; Polymeropoulos, M H; Moses, S W; Parvari, R; Hershkovitz, E; Mandel, H; Fryman, M; Chou, J Y

1998-02-01

Glycogen-storage disease type 1 (GSD-1), also known as "von Gierke disease," is caused by a deficiency in microsomal glucose-6-phosphatase (G6Pase) activity. There are four distinct subgroups of this autosomal recessive disorder: 1a, 1b, 1c, and 1d. All share the same clinical manifestations, which are caused by abnormalities in the metabolism of glucose-6-phosphate (G6P). However, only GSD-1b patients suffer infectious complications, which are due to both the heritable neutropenia and the functional deficiencies of neutrophils and monocytes. Whereas G6Pase deficiency in GSD-1a patients arises from mutations in the G6Pase gene, this gene is normal in GSD-1b patients, indicating a separate locus for the disorder in the 1b subgroup. We now report the linkage of the GSD-1b locus to genetic markers spanning a 3-cM region on chromosome 11q23. Eventual molecular characterization of this disease will provide new insights into the genetic bases of G6P metabolism and neutrophil-monocyte dysfunction.

Large animal models and new therapies for glycogen storage disease.

PubMed

Brooks, Elizabeth D; Koeberl, Dwight D

2015-05-01

Glycogen storage diseases (GSD), a unique category of inherited metabolic disorders, were first described early in the twentieth century. Since then, the biochemical and genetic bases of these disorders have been determined, and an increasing number of animal models for GSD have become available. At least seven large mammalian models have been developed for laboratory research on GSDs. These models have facilitated the development of new therapies, including gene therapy, which are undergoing clinical translation. For example, gene therapy prolonged survival and prevented hypoglycemia during fasting for greater than one year in dogs with GSD type Ia, and the need for periodic re-administration to maintain efficacy was demonstrated in that dog model. The further development of gene therapy could provide curative therapy for patients with GSD and other inherited metabolic disorders.

A comparison of bleeding patterns and cycle control using two transdermal contraceptive systems: a multicenter, open-label, randomized study.

PubMed

Gruber, D; Skřivánek, A; Serrani, M; Lanius, V; Merz, M

2015-02-01

To investigate the bleeding pattern and cycle control parameters of a contraceptive patch containing 0.55 mg ethinyl estradiol (EE) and 2.1 mg gestodene (GSD) compared with a patch containing 0.6 mg EE and 6 mg norelgestromin (NGMN). In this phase III, open-label, randomized, parallel-group trial, healthy women aged 18-35 years (smokers aged 18-30 years) received either the EE/GSD patch (n=200) or the EE/NGMN patch (n=198). Treatment consisted of one patch per week for 3 weeks followed by a 7-day, patch-free interval for seven cycles. Bleeding control was assessed in two 90-day reference periods. In reference period 1, mean number of bleeding/spotting days was comparable across treatment groups (p>0.05). However, in reference period 2, there were fewer bleeding/spotting days in the EE/GSD patch group (15.7 versus 18.4; p<0.0001). Mean number of bleeding/spotting episodes was comparable across groups for both reference periods, but bleeding/spotting episodes were shorter for the EE/GSD patch than the EE/NGMN patch during reference period 1 (5.13 days versus 5.53 days, respectively; p<0.05) and reference period 2 (5.07 versus 5.66; p=0.0001). Both treatment groups showed a similar frequency of withdrawal bleeding episodes; however, across all seven cycles, the length of these episodes was consistently shorter with the EE/GSD patch (p<0.01). There were no notable treatment differences in intracyclic bleeding. Bleeding pattern and cycle control achieved with the EE/GSD patch was similar to that of the EE/NGMN patch. The paper presents data on the bleeding pattern and cycle control parameters of an investigational transdermal contraceptive patch containing EE and GSD compared with an approved contraceptive patch containing EE and NGMN. This descriptive study found that bleeding patterns associated with the EE/GSD patch were similar to those of an EE/NGMN patch providing higher EE exposure. Copyright © 2015 Elsevier Inc. All rights reserved.

Comparison of selected endocrine parameters during luteal phase and pregnancy in German Shepherd dogs and Beagles.

PubMed

Günzel-Apel, A R; Beste, N; Nottorf, S; Eschricht, F; Hoppen, H O; Dieleman, S; Einspanier, A

2009-07-01

Concentrations of progesterone, prolactin and relaxin in serum at predetermined intervals after ovulation (day 0) in non-pregnant and pregnant normocyclic Beagles were assayed and results compared with those observed in German Shepherd dogs (GSD) in a previous study. The goal was to determine possible reproductive hormone specificities related to the GSD breed. Furthermore, the effects of medroxyprogesterone acetate (MPA)-treatment in non-pregnant Beagles and of progesterone supplementation in pregnant Beagles on the hormone concentrations were examined. Mean concentrations of progesterone and prolactin were not different in the non-pregnant Beagles compared with those seen in non-pregnant GSD, except at days 50-60, when progesterone concentrations were found to be higher in Beagles (p < 0.05). Mean progesterone concentrations in pregnant Beagles at days 50-60 after ovulation (day 0) were higher (p < 0.05) than in GSD at that time, but not at earlier time periods. Prolactin concentrations were higher (p < 0.05) in Beagles throughout pregnancy compared with those in the GSD. Mean relaxin concentrations were numerically but not significantly lower in GSD than in Beagles throughout pregnancy. A 10-day oral MPA treatment did not affect progesterone or prolactin secretion in normocyclic non-pregnant Beagles. Medroxyprogesterone acetate serum concentrations were approximately 3.9 ng/ml during treatment and decreased to 0.42 and 0.021 ng/ml within 5 and 15 days after end of treatment, respectively. Intramuscular progesterone supplementation from days 30 to 40 in pregnant Beagles resulted in higher concentrations of progesterone in the 36- to 45-day time periods; prolactin and relaxin concentrations were not significantly affected during or after treatment compared with administration of placebo. The results suggest a tendency towards deficient luteal function in the short-cycle GSD bitches previously studied, which in pregnancy may reflect the observed decreased prolactin concentrations; the possibility that GSD relaxin secretion is deficiency required needs further study. As oral treatment with MPA did not affect progesterone and prolactin release, it may be useful for studying luteal function in pregnant bitches with suspected hypoluteoidism.

Clinical and biochemical heterogeneity between patients with glycogen storage disease type IA: the added value of CUSUM for metabolic control.

PubMed

Peeks, Fabian; Steunenberg, Thomas A H; de Boer, Foekje; Rubio-Gozalbo, M Estela; Williams, Monique; Burghard, Rob; Rajas, Fabienne; Oosterveer, Maaike H; Weinstein, David A; Derks, Terry G J

2017-09-01

To study heterogeneity between patients with glycogen storage disease type Ia (GSD Ia), a rare inherited disorder of carbohydrate metabolism caused by the deficiency of glucose-6-phosphatase (G6Pase). Descriptive retrospective study of longitudinal clinical and biochemical data and long-term complications in 20 GSD Ia patients. We included 11 patients with homozygous G6PC mutations and siblings from four families carrying identical G6PC genotypes. To display subtle variations for repeated triglyceride measurements with respect to time for individual patients, CUSUM-analysis graphs were constructed. Patients with different homozygous G6PC mutations showed important differences in height, BMI, and biochemical parameters (i.e., lactate, uric acid, triglyceride, and cholesterol concentrations). Furthermore, CUSUM-analysis predicts and displays subtle changes in longitudinal blood triglyceride concentrations. Siblings in families also displayed important differences in biochemical parameters (i.e., lactate, uric acid, triglycerides, and cholesterol concentrations) and long-term complications (i.e., liver adenomas, nephropathy, and osteopenia/osteoporosis). Differences between GSD Ia patients reflect large clinical and biochemical heterogeneity. Heterogeneity between GSD Ia patients with homozygous G6PC mutations indicate an important role of the G6PC genotype/mutations. Differences between affected siblings suggest an additional role (genetic and/or environmental) of modifying factors defining the GSD Ia phenotype. CUSUM-analysis can facilitate single-patient monitoring of metabolic control and future application of this method may improve precision medicine for patients both with GSD and remaining inherited metabolic diseases.

[Glycogen storage disease type Ⅰa: a rare cause of gout in adolescent and young adult patients].

PubMed

Xu, N; Huang, X M; Fang, W G; Zhang, Y; Qiu, Z Q; Zeng, X J

2018-04-01

Objective: To analyze the clinical features of secondary gout in glycogen storage disease type Ⅰa (GSD Ⅰa), so as to improve the awareness of this disease. Methods: The clinical features, laboratory findings, treatments and prognosis of 5 GSD Ⅰa patients with secondary gout who had been admitted to the Peking Union Medical College Hospital during 2006 to 2016 were collected and analyzed. GSD Ⅰa was confirmed by liver biopsy and genotyping. Results: Among the 5 patients (median age: 27 years), 3 were males and 2 were females. The mean age of gout onset was 17 ranging from 10 to 22 years old. The common manifestations of GSD included hepatomegaly since childhood, hypoglycemia, growth retardation, anemia, hyperlactacidemia and hyperlipidemia. All the 5 patients were complicated with gouty tophi and kidney stone. Gouty tophi and kidney stone were identified 3.8 years and 10.2 years after the first occurrence of articular symptoms, respectively. Renal damage occurred in 3 cases. All the patients underwent several therapeutic modalities including lifestyle intervention, allopurinol, and raw corn starch treatment. Conclusions: Determination of the presence of primary disease should be performed actively for young-onset gout with early occurrence of gouty tophi. GSD should be suspected if there exist clinical manifestations like hepatomegaly, recurrent hypoglycemia, growth retardation. Early management of hyperuricemia and gout in GSD patients is important to prevent complications and improve prognosis.

Evaluation of solar sludge drying alternatives by costs and area requirements.

PubMed

Kurt, Mayıs; Aksoy, Ayşegül; Sanin, F Dilek

2015-10-01

Thermal drying is a common method to reach above 90% dry solids content (DS) in sludge. However, thermal drying requires high amount of energy and can be expensive. A greenhouse solar dryer (GSD) can be a cost-effective substitute if the drying performance, which is typically 70% DS, can be increased by additional heat. In this study feasibility of GSD supported with solar panels is evaluated as an alternative to thermal dryers to reach 90% DS. Evaluations are based on capital and O&M costs as well as area requirements for 37 wastewater treatment plants (WWTPs) with various sludge production rates. Costs for the supported GSD system are compared to that of conventional and co-generation thermal dryers. To calculate the optimal costs associated with the drying system, an optimization model was developed in which area limitation was a constraint. Results showed that total cost was minimum when the DS in the GSD (DS(m,i)) was equal to the maximum attainable value (70% DS). On average, 58% of the total cost and 38% of total required area were associated with the GSD. Variations in costs for 37 WWTPs were due to differences in initial DS (DS(i,i)) and sludge production rates, indicating the importance of dewatering to lower drying costs. For large plants, GSD supported with solar panels provided savings in total costs especially in long term when compared to conventional and co-generation thermal dryers. Copyright © 2015 Elsevier Ltd. All rights reserved.

Molecular diagnosis of glycogen storage disease and disorders with overlapping clinical symptoms by massive parallel sequencing.

PubMed

Vega, Ana I; Medrano, Celia; Navarrete, Rosa; Desviat, Lourdes R; Merinero, Begoña; Rodríguez-Pombo, Pilar; Vitoria, Isidro; Ugarte, Magdalena; Pérez-Cerdá, Celia; Pérez, Belen

2016-10-01

Glycogen storage disease (GSD) is an umbrella term for a group of genetic disorders that involve the abnormal metabolism of glycogen; to date, 23 types of GSD have been identified. The nonspecific clinical presentation of GSD and the lack of specific biomarkers mean that Sanger sequencing is now widely relied on for making a diagnosis. However, this gene-by-gene sequencing technique is both laborious and costly, which is a consequence of the number of genes to be sequenced and the large size of some genes. This work reports the use of massive parallel sequencing to diagnose patients at our laboratory in Spain using either a customized gene panel (targeted exome sequencing) or the Illumina Clinical-Exome TruSight One Gene Panel (clinical exome sequencing (CES)). Sequence variants were matched against biochemical and clinical hallmarks. Pathogenic mutations were detected in 23 patients. Twenty-two mutations were recognized (mostly loss-of-function mutations), including 11 that were novel in GSD-associated genes. In addition, CES detected five patients with mutations in ALDOB, LIPA, NKX2-5, CPT2, or ANO5. Although these genes are not involved in GSD, they are associated with overlapping phenotypic characteristics such as hepatic, muscular, and cardiac dysfunction. These results show that next-generation sequencing, in combination with the detection of biochemical and clinical hallmarks, provides an accurate, high-throughput means of making genetic diagnoses of GSD and related diseases.Genet Med 18 10, 1037-1043.

[Characteristics of lipid metabolism and the cardiovascular system in glycogenosis types I and III].

PubMed

Polenova, N V; Strokova, T V; Starodubova, A V

Glycogen storage disease (GSD) is an inherited metabolic disorder characterized by early childhood lipid metabolic disturbances with potentially proatherogenic effects. The review outlines the characteristics of impaired lipid composition and other changes in the cardiovascular system in GSD types I and III. It analyzes the factors enabling and inhibiting the development of atherosclerosis in patients with GSD. The review describes the paradox of vascular resistance to the development of early atherosclerosis despite the proatherogenic composition of lipids in the patients of this group.

Pathogenesis of growth failure and partial reversal with gene therapy in murine and canine Glycogen Storage Disease type Ia.

PubMed

Brooks, Elizabeth Drake; Little, Dianne; Arumugam, Ramamani; Sun, Baodong; Curtis, Sarah; Demaster, Amanda; Maranzano, Michael; Jackson, Mark W; Kishnani, Priya; Freemark, Michael S; Koeberl, Dwight D

2013-06-01

Glycogen Storage Disease type Ia (GSD-Ia) in humans frequently causes delayed bone maturation, decrease in final adult height, and decreased growth velocity. This study evaluates the pathogenesis of growth failure and the effect of gene therapy on growth in GSD-Ia affected dogs and mice. Here we found that homozygous G6pase (-/-) mice with GSD-Ia have normal growth hormone (GH) levels in response to hypoglycemia, decreased insulin-like growth factor (IGF) 1 levels, and attenuated weight gain following administration of GH. Expression of hepatic GH receptor and IGF 1 mRNAs and hepatic STAT5 (phospho Y694) protein levels are reduced prior to and after GH administration, indicating GH resistance. However, restoration of G6Pase expression in the liver by treatment with adeno-associated virus 8 pseudotyped vector expressing G6Pase (AAV2/8-G6Pase) corrected body weight, but failed to normalize plasma IGF 1 in G6pase (-/-) mice. Untreated G6pase (-/-) mice also demonstrated severe delay of growth plate ossification at 12 days of age; those treated with AAV2/8-G6Pase at 14 days of age demonstrated skeletal dysplasia and limb shortening when analyzed radiographically at 6 months of age, in spite of apparent metabolic correction. Moreover, gene therapy with AAV2/9-G6Pase only partially corrected growth in GSD-Ia affected dogs as detected by weight and bone measurements and serum IGF 1 concentrations were persistently low in treated dogs. We also found that heterozygous GSD-Ia carrier dogs had decreased serum IGF 1, adult body weights and bone dimensions compared to wild-type littermates. In sum, these findings suggest that growth failure in GSD-Ia results, at least in part, from hepatic GH resistance. In addition, gene therapy improved growth in addition to promoting long-term survival in dogs and mice with GSD-Ia. Copyright © 2013 Elsevier Inc. All rights reserved.

Pathogenesis of growth failure and partial reversal with gene therapy in murine and canine Glycogen Storage Disease type Ia

PubMed Central

Brooks, Elizabeth Drake; Little, Dianne; Arumugam, Ramamani; Sun, Baodong; Curtis, Sarah; DeMaster, Amanda; Maranzano, Michael; Jackson, Mark W.; Kishnani, Priya; Freemark, Michael S.; Koeberl, Dwight D.

2013-01-01

Glycogen Storage Disease type Ia (GSD-Ia) in humans frequently causes delayed bone maturation, decrease in final adult height, and decreased growth velocity. This study evaluates the pathogenesis of growth failure and the effect of gene therapy on growth in GSD-Ia affected dogs and mice. Here we found that homozygous G6pase (−/−) mice with GSD-Ia have normal growth hormone (GH) levels in response to hypoglycemia, decreased insulin-like growth factor (IGF) 1 levels, and attenuated weight gain following administration of GH. Expression of hepatic GH receptor and IGF 1 mRNAs and hepatic STAT5 (phospho Y694) protein levels are reduced prior to and after GH administration, indicating GH resistance. However, restoration of G6Pase expression in the liver by treatment with adeno-associated virus 8 pseudotyped vector expressing G6Pase (AAV2/8-G6Pase) corrected body weight, but failed to normalize plasma IGF 1 in G6pase (−/−) mice. Untreated G6pase (−/−) mice also demonstrated severe delay of growth plate ossification at 12 days of age; those treated with AAV2/8-G6Pase at 14 days of age demonstrated skeletal dysplasia and limb shortening when analyzed radiographically at 6 months of age, in spite of apparent metabolic correction. Moreover, gene therapy with AAV2/9-G6Pase only partially corrected growth in GSD-Ia affected dogs as detected by weight and bone measurements and serum IGF 1 concentrations were persistently low in treated dogs. We also found that heterozygous GSD-Ia carrier dogs had decreased serum IGF 1, adult body weights and bone dimensions compared to wild-type littermates. In sum, these findings suggest that growth failure in GSD-Ia results, at least in part, from hepatic GH resistance. In addition, gene therapy improved growth in addition to promoting long-term survival in dogs and mice with GSD-Ia. PMID:23623482

Glycogen storage disease type 1a in three siblings with the G270V mutation.

PubMed

Parvari, R; Isam, J; Moses, S W

1999-04-01

Glycogen storage disease type 1a (von Gierke disease, GSD1a) is caused by the deficiency of microsomal glucose-6-phosphatase (G6Pase) activity. The cloning of G6Pase cDNA and characterization of the human G6Pase gene enabled the identification of the mutations causing GSD1a. Here we report on the clinical and biochemical features of three GSD1a siblings of a Muslin Arab family with a G270V mutation. Two older patients presented with an unusually mild clinical and biochemical course.

Rescue administration of a helper-dependent adenovirus vector with long-term efficacy in dogs with glycogen storage disease type Ia.

PubMed

Crane, B; Luo, X; Demaster, A; Williams, K D; Kozink, D M; Zhang, P; Brown, T T; Pinto, C R; Oka, K; Sun, F; Jackson, M W; Chan, L; Koeberl, D D

2012-04-01

Glycogen storage disease type Ia (GSD-Ia) stems from glucose-6-phosphatase (G6Pase) deficiency and causes hypoglycemia, hepatomegaly, hypercholesterolemia and lactic acidemia. Three dogs with GSD-Ia were initially treated with a helper-dependent adenovirus encoding a human G6Pase transgene (HDAd-cG6Pase serotype 5) on postnatal day 3. Unlike untreated dogs with GSD-Ia, all three dogs initially maintained normal blood glucose levels. After 6-22 months, vector-treated dogs developed hypoglycemia, anorexia and lethargy, suggesting that the HDAd-cG6Pase serotype 5 vector had lost efficacy. Liver biopsies collected at this time revealed significantly elevated hepatic G6Pase activity and reduced glycogen content, when compared with affected dogs treated only by frequent feeding. Subsequently, the HDAd-cG6Pase serotype 2 vector was administered to two dogs, and hypoglycemia was reversed; however, renal dysfunction and recurrent hypoglycemia complicated their management. Administration of a serotype 2 HDAd vector prolonged survival in one GSD-Ia dog to 12 months of age and 36 months of age in the other, but the persistence of long-term complications limited HDAd vectors in the canine model for GSD-Ia.

Exercise in muscle glycogen storage diseases.

PubMed

Preisler, Nicolai; Haller, Ronald G; Vissing, John

2015-05-01

Glycogen storage diseases (GSD) are inborn errors of glycogen or glucose metabolism. In the GSDs that affect muscle, the consequence of a block in skeletal muscle glycogen breakdown or glucose use, is an impairment of muscular performance and exercise intolerance, owing to 1) an increase in glycogen storage that disrupts contractile function and/or 2) a reduced substrate turnover below the block, which inhibits skeletal muscle ATP production. Immobility is associated with metabolic alterations in muscle leading to an increased dependence on glycogen use and a reduced capacity for fatty acid oxidation. Such changes may be detrimental for persons with GSD from a metabolic perspective. However, exercise may alter skeletal muscle substrate metabolism in ways that are beneficial for patients with GSD, such as improving exercise tolerance and increasing fatty acid oxidation. In addition, a regular exercise program has the potential to improve general health and fitness and improve quality of life, if executed properly. In this review, we describe skeletal muscle substrate use during exercise in GSDs, and how blocks in metabolic pathways affect exercise tolerance in GSDs. We review the studies that have examined the effect of regular exercise training in different types of GSD. Finally, we consider how oral substrate supplementation can improve exercise tolerance and we discuss the precautions that apply to persons with GSD that engage in exercise.

Long-term safety and efficacy of AAV gene therapy in the canine model of glycogen storage disease type Ia.

PubMed

Lee, Young Mok; Conlon, Thomas J; Specht, Andrew; Coleman, Kirsten E; Brown, Laurie M; Estrella, Ana M; Dambska, Monika; Dahlberg, Kathryn R; Weinstein, David A

2018-05-25

Viral mediated gene therapy has progressed after overcoming early failures, and gene therapy has now been approved for several conditions in Europe and the USA. Glycogen storage disease (GSD) type Ia, caused by a deficiency of glucose-6-phosphatase-α, has been viewed as an outstanding candidate for gene therapy. This follow-up report describes the long-term outcome for the naturally occurring GSD-Ia dogs treated with rAAV-GPE-hG6PC-mediated gene therapy. A total of seven dogs were treated with rAAV-GPE-hG6PC-mediated gene therapy. The first four dogs were treated at birth, and three dogs were treated between 2 and 6 months of age to assess the efficacy and safety in animals with mature livers. Blood and urine samples, radiographic studies, histological evaluation, and biodistribution were assessed. Gene therapy improved survival in the GSD-Ia dogs. With treatment, the biochemical studies normalized for the duration of the study (up to 7 years). None of the rAAV-GPE-hG6PC-treated dogs had focal hepatic lesions or renal abnormalities. Dogs treated at birth required a second dose of rAAV after 2-4 months; gene therapy after hepatic maturation resulted in improved efficacy after a single dose. rAAV-GPE-hG6PC treatment in GSD-Ia dogs was found to be safe and efficacious. GSD-Ia is an attractive target for human gene therapy since it is a monogenic disorder with limited tissue involvement. Blood glucose and lactate monitoring can be used to assess effectiveness and as a biomarker of success. GSD-Ia can also serve as a model for other hepatic monogenic disorders.

Glycogen storage disease type 1a in Israel: biochemical, clinical, and mutational studies.

PubMed

Parvari, R; Lei, K J; Bashan, N; Hershkovitz, E; Korman, S H; Barash, V; Lerman-Sagie, T; Mandel, H; Chou, J Y; Moses, S W

1997-10-31

Glycogen storage disease type 1a (von Gierke disease, GSD 1a) is caused by the deficiency of microsomal glucose-6-phosphatase (G6Pase) activity which catalyzes the final common step of glycogenolysis and gluconeogenesis. The recent cloning of the G6Pase cDNA and characterization of the human G6Pase gene enabled the characterization of the mutations causing GSD 1a. This, in turn, allows the introduction of a noninvasive DNA-based diagnosis that provides reliable carrier testing and prenatal diagnosis. In this study, we report the biochemical and clinical characteristics as well as mutational analyses of 12 Israeli GSD 1a patients of different families, who represent most GSD 1a patients in Israel. The mutations, G6Pase activity, and glycogen content of 7 of these patients were reported previously. The biochemical data and clinical findings of all patients were similar and compatible with those described in other reports. All 9 Jewish patients, as well as one Muslim Arab patient, presented the R83C mutation. Two Muslim Arab patients had the V166G mutation which was not found in other patients' populations. The V166G mutation, which was introduced into the G6Pase cDNA by site-directed mutagenesis following transient expression in COS-1 cells, was shown to cause complete inactivation of the G6Pase. The characterization of all GSD 1a mutations in the Israeli population lends itself to carrier testing in these families as well as to prenatal diagnosis, which was carried out in 2 families. Since all Ashkenzai Jewish patients harbor the same mutation, our study suggests that DNA-based diagnosis may be used as an initial diagnostic step in Ashkenazi Jews suspected of having GSD 1a, thereby avoiding liver biopsy.

Improvement of Cardiomyopathy After High-Fat Diet in Two Siblings with Glycogen Storage Disease Type III.

PubMed

Brambilla, Alessandra; Mannarino, Savina; Pretese, Roberta; Gasperini, Serena; Galimberti, Cinzia; Parini, Rossella

2014-01-01

Glycogenosis type III (GSD III) is an autosomal recessive disorder due to amylo-1,6-glucosidase deficiency. This disease causes limit dextrin storage in affected tissues: liver, skeletal muscles, and heart in GSD IIIa and only liver in GSD IIIb. Cardiomyopathy is quite frequent in GSD IIIa with variable severity and progression of manifestations. It is not clear if diet manipulation may interfere with cardiomyopathy's progression. Recent case reports showed improvement of cardiomyopathy following a ketogenic diet.Two siblings (girl and boy), 7- and 5-year-old, both affected with GSD IIIa, developed severe and rapidly worsening left ventricular hypertrophy in the first years of life, while treated with frequent diurnal and nocturnal hyperproteic meals followed by orally administered uncooked cornstarch. Subsequently they were treated with high-fat (60%) and high-protein (25%), low-carbohydrate (15%) diet. After 12 months exertion dyspnea disappeared in the girl and biochemical blood tests, cardiac enzymes, and congestive heart failure markers improved in both (CK 3439→324, 1304→581 U/L; NT-proBNP 2084→206, 782→135 pg/mL, respectively); ultrasound assessment in both patients showed a relevant reduction of the thickness of interventricular septum (30→16, 16→11 mm, respectively) and left ventricle posterior wall (18→7, 13→8 mm, respectively) and an improvement of the outflow obstruction. A diet rich in fats as well as proteins and poor in carbohydrates could be a beneficial therapeutic choice for GSD III with cardiomyopathy. Future research is needed to confirm the beneficial effect of this treatment and to design treatment strategies with the aim to provide alternative source of energy and prevent glycogen accumulation.

Microstructure and Mechanical Properties of Ti-6Al-4V Fabricated by Selective Laser Melting of Powder Produced by Granulation-Sintering-Deoxygenation Method

NASA Astrophysics Data System (ADS)

Sun, Pei; Fang, Z. Zak; Zhang, Ying; Xia, Yang

2017-12-01

Commercial spherical Ti powders for additive manufacturing applications are produced today by melt-atomization methods at relatively high costs. A meltless production method, called granulation-sintering-deoxygenation (GSD), was developed recently to produce spherical Ti alloy powder at a significantly reduced cost. In this new process, fine hydrogenated Ti particles are agglomerated to form spherical granules, which are then sintered to dense spherical particles. After sintering, the solid fully dense spherical Ti alloy particles are deoxygenated using novel low-temperature deoxygenation processes with either Mg or Ca. This technical communication presents results of 3D printing using GSD powder and the selective laser melting (SLM) technique. The results showed that tensile properties of parts fabricated from spherical GSD Ti-6Al-4V powder by SLM are comparable with typical mill-annealed Ti-6Al-4V. The characteristics of 3D printed Ti-6Al-4V from GSD powder are also compared with that of commercial materials.

Evaluation of central nervous system in patients with glycogen storage disease type 1a.

PubMed

Aydemir, Yusuf; Gürakan, Figen; Saltık Temizel, İnci Nur; Demir, Hülya; Oğuz, Kader Karlı; Yalnızoğlu, Dilek; Topçu, Meral; Özen, Hasan; Yüce, Aysel

2016-01-01

We aimed to evaluate structure and functions of central nervous system (CNS) in children with glycogen storage disease (GSD) type 1a. Neurological examination, psychometric tests, electroencephalography (EEG), magnetic resonance imaging (MRI), visual evoked potentials (VEP) and brainstem auditory evoked potentials (BAEP) were performed. The results were compared between patients with good and poor metabolic control and healthy children. Twenty-three patients with GSD type 1a were studied. Twelve patients were in poor metabolic control group and 11 patients in good metabolic control group. Five patients had intellectual disability, 10 had EEG abnormalities, seven had abnormal VEP and two had abnormal BAEP results. MRI was abnormal in five patients. There was significant correlation between the number of hypoglycemic attacks and MRI abnormalities. Central nervous system may be affected in GSD type 1a even in patients with normal neurologic examination. Accumulation of abnormal results in patients with poor metabolic control supports the importance of metabolic control in GSD type 1a.

Glycogen storage disease type III in Israel: presentation and long-term outcome.

PubMed

Hershkovitz, Eli; Forschner, Itay; Mandel, Hanna; Spiegel, Ronen; Lerman-Sagie, Tally; Anikster, Yair; Zeharia, A; Moses, Shimon

2014-03-01

Glycogen storage disease type III (GSD III) was found in the past with an unusual frequency among North African Jews in Israel. The aim of this study was to review the long-term clinical course of GSD III's patients in Israel. Relevant pediatric and adult clinical units of all Israeli hospitals were approached to report on their GSD III patients. 21 (14 M/7F) live patients were located. The average age of the patients was nearly twenty years. Eleven patients were older than 18 years of age. 76% of the patients were of Jewish North African origin, 14% of Jewish European origin, and 10% were Arab Muslims. The symptoms at presentation were fasting, hypoglycemia, hepatomegaly slight hypotonia in infancy and delayed growth. Although in most of the patients their signs and symptoms ameliorated after childhood, significant complications were observed in some 20% of the patients. Consequently, a life long follow up of GSD-III patients is required.

Pregnancies in Glycogen Storage Disease Type Ia

PubMed Central

Martens, Daniëlle HJ; Rake, Jan Peter; Schwarz, Martin; Ullrich, Kurt; Weinstein, David A; Merkel, Martin; Sauer, Pieter JJ; Smit, G Peter A

2013-01-01

Objective Reports on pregnancies in women with GSD-Ia are scarce. Due to improved life expectancy, pregnancy is becoming an important issue. We describe 15 pregnancies focusing on dietary treatment, biochemical parameters and GSD-Ia complications. Study Design Carbohydrate requirements (mg/kg/min), triglyceride and uric acid levels, liver ultrasonography and creatinine clearance were investigated before, during and after pregnancy. Data of the newborns were obtained from the records. Results In the first trimester, a significant increase in carbohydrate requirements was observed (p=0,007). Most patients had acceptable triglyceride and uric acid levels during pregnancy. No increase in size/number of adenomas was seen. In 3/4 patients, a decrease in GFR was observed after pregnancy. In three pregnancies, lactic acidosis developed during delivery with severe multi-organ failure in one. All but one of the children are healthy and show good psychomotor development. Conclusion Successful pregnancies are possible in GSD-Ia patients, although specific GSD-Ia related risks are present. PMID:18241814

Antibodies to CBir1 are Associated with Glycogen Storage Disease Type Ib

PubMed Central

Davis, Michael K.; Valentine, John F.; Weinstein, David A.; Polyak, Steven

2009-01-01

Objectives Glycogen storage disease (GSD) type Ib is a congenital disorder of glycogen metabolism that is associated with neutropenia, neutrophil and monocyte dysfunction, and an inflammatory bowel disease (IBD) that mimics a Crohn's disease phenotype. The enteric microflora is implicated in the pathogenesis of IBD; however, its role in the development of GSD-associated IBD is unknown. Antibody reactivity to Saccharomyces cerevisiae antibodies (ASCA), Escherichia coli outer membrane porin C (anti-OmpC) and bacterial flagellin (anti-CBir1) have been associated with Crohn's disease in the general population, but they have an undetermined association in children and adults with GSD-Ib. Our goal was to examine the association of ASCA, anti-OmpC and anti-CBir1 with the clinical features of GSD-Ib enterocolitis. Methods A retrospective review identified 19 GSD-Ib patients with or without a known diagnosis of enterocolitis. Radiographic, endoscopic and serologic data were collected and assays for ASCA, anti-OmpC, and anti-CBir1 obtained. Results Seven patients had combined radiographic, endoscopic, and histological evidence of intestinal inflammation; the majority had ileocolonic involvement. Seventeen of 19 (89%) patients had elevated anti-CBir1 levels (6/7 in the IBD group and 11/12 in the no clinical evidence of IBD group). Thirteen of 19 (68%) had elevated anti-OmpC levels (5/7 in the IBD group and 8/12 in the no clinical evidence of IBD group). Eleven of 19 (58%) patients had elevated ASCA IgA levels (4/7 in the IBD group and 7/12 in the no clinical evidence of IBD group). Conclusion Nearly all of the GSD type Ib patients had elevated anti-CBir1 levels. The antibody did not differentiate those with and without a diagnosis of GSD-Ib-associated IBD. Seroreactivity to flagellin may represent immune dysfunction rather than active enterocolitis in this patient population. Long-term follow-up of the group without known IBD is required to determine if these antibodies can predict intestinal inflammation. PMID:20410847

Association between different combination of measures for obesity and new-onset gallstone disease.

PubMed

Liu, Tong; Wang, Wanchao; Ji, Yannan; Wang, Yiming; Liu, Xining; Cao, Liying; Liu, Siqing

2018-01-01

Body mass index(BMI) is a calculation index of general obesity. Waist circumference(WC) is a measure of body-fat distribution and always used to estimate abdominal obesity. An important trait of general obesity and abdominal obesity is their propensity to coexist. Using one single measure of obesity could not estimate persons at risk for GSD precisely. This study aimed to compare the predictive values of various combination of measures for obesity(BMI, WC, waist to hip ratio) for new-onset GSD. We prospectively studied the predictive values of various combination of measures for obesity for new-onset GSD in a cohort of 88,947 participants who were free of prior gallstone disease, demographic characteristics and biochemical parameters were recorded. 4,329 participants were identified to have GSD among 88,947 participants during 713 345 person-years of follow-up. Higher BMI, WC and waist to hip ratio (WHtR) were significantly associated with higher risks of GSD in both genders even after adjustment for potential confounders. In males, the hazard ratio for the highest versus lowest BMI, WC, WHtR were 1.63(1.47~1.79), 1.53(1.40~1.68), 1.44(1.31~1.58), respectively. In females, the hazard ratio for the highest versus lowest BMI, WC, WHtR were 2.11(1.79~2.49), 1.85(1.55~2.22), 1.84(1.55~2.19), respectively. In male group, the combination of BMI+WC improved the predictive ability of the model more clearly than other combinations after adding them to the multivariate model in turn, while for females the best predictive combination was BMI+WHtR. Elevated BMI, WC and WHtR were independent risk factors for new-onset GSD in both sex groups after additional adjustment was made for potential confounders. In males, the combination of BMI+WC seemed to be the most predictable model to evaluate the effect of obesity on new-onset GSD, while the best combination in females was BMI+WHtR.

78 FR 10001 - Proposed Collection; Comment Request for Form 706-GS(D)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-12

... Form 706-GS(D), Generation-Skipping Transfer Tax Return for Distributions. DATES: Written comments should be received on or before April 15, 2013 to be assured of consideration. ADDRESSES: Direct all... [email protected] . SUPPLEMENTARY INFORMATION: Title: Generation-Skipping Transfer Tax Return for...

Improving glycaemic control and life skills in adolescents with type 1 diabetes: A randomised, controlled intervention study using the Guided Self-Determination-Young method in triads of adolescents, parents and health care providers integrated into routine paediatric outpatient clinics

PubMed Central

2011-01-01

Background Adolescents with type 1 diabetes face demanding challenges due to conflicting priorities between psychosocial needs and diabetes management. This conflict often results in poor glycaemic control and discord between adolescents and parents. Adolescent-parent conflicts are thus a barrier for health care providers (HCPs) to overcome in their attempts to involve both adolescents and parents in improvement of glycaemic control. Evidence-based interventions that involve all three parties (i.e., adolescents, parents and HCPs) and are integrated into routine outpatient clinic visits are lacking. The Guided Self-Determination method is proven effective in adult care and has been adapted to adolescents and parents (Guided Self-Determination-Young (GSD-Y)) for use in paediatric diabetes outpatient clinics. Our objective is to test whether GSD-Y used in routine paediatric outpatient clinic visits will reduce haemoglobin A1c (HbA1c) concentrations and improve adolescents' life skills compared with a control group. Methods/Design Using a mixed methods design comprising a randomised controlled trial and a nested qualitative evaluation, we will recruit 68 adolescents age 13 - 18 years with type 1 diabetes (HbA1c > 8.0%) and their parents from 2 Danish hospitals and randomise into GSD-Y or control groups. During an 8-12 month period, the GSD-Y group will complete 8 outpatient GSD-Y visits, and the control group will completes an equal number of standard visits. The primary outcome is HbA1c. Secondary outcomes include the following: number of self-monitored blood glucose values and levels of autonomous motivation, involvement and autonomy support from parents, autonomy support from HCPs, perceived competence in managing diabetes, well-being, and diabetes-related problems. Primary and secondary outcomes will be evaluated within and between groups by comparing data from baseline, after completion of the visits, and again after a 6-month follow-up. To illustrate how GSD-Y influences glycaemic control and the development of life skills, 10-12 GSD-Y visits will be recorded during the intervention and analysed qualitatively together with individual interviews carried out after follow-up. Discussion This study will provide evidence of the effectiveness of using a GSD-Y intervention with three parties on HbA1c and life skills and the feasibility of integrating the intervention into routine outpatient clinic visits. Danish Data Association ref nr. 2008-41-2322 Trial registration ISRCTN54243636 PMID:21672252

76 FR 64403 - Self-Regulatory Organizations; The Fixed Income Clearing Corporation; Notice of Filing and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-18

... Schedule of Timeframes, and Fee Structure in the GSD Rules. II. Self-Regulatory Organization's Statement of...-Regulatory Organizations; The Fixed Income Clearing Corporation; Notice of Filing and Immediate Effectiveness... Timeframes, and Fee Structure in the GSD Rules October 12, 2011. Pursuant to Section 19(b)(1) of the...

78 FR 42809 - Self-Regulatory Organizations; Fixed Income Clearing Corporation; Order Approving Proposed Rule...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-17

... remain subject to all of the GSD's normal risk management procedures, which include marking member portfolios to the market on an intraday basis and charging variation margins accordingly.\\19\\ These risk management procedures should help ensure the safety of the securities and funds handled by the GSD in...

Gene Therapy Vector for the Treatment of Glycogen Storage Disease Type Ia (GSD-Ia) | NCI Technology Transfer Center | TTC

Cancer.gov

GSD-Ia is an inherited disorder of metabolism associated with life-threatening hypoglycemia, hepatic malignancy, and renal failure caused by the deficiency of glucose-6-phosphatase-alpha (G6Pase-alpha or G6PC). NICHD seeks parties to license this invention towards commercialization.

Association of green stem disorder with agronomic traits in soybean

USDA-ARS?s Scientific Manuscript database

Green stem disorder of soybean (GSD) is the occurrence of non-senescent, fleshy green stems of plants with normal, fully mature pods and seeds. Data on GSD incidence based on a percentage of plants in plots showing symptoms were collected for soybean cultivars in 86 trials from 2009 to 2012 at seven...

Molecular marker suggests rapid changes of sex-determining mechanisms in Australian dragon lizards.

PubMed

Ezaz, Tariq; Quinn, Alexander E; Sarre, Stephen D; O'Meally, Denis; Georges, Arthur; Graves, Jennifer A Marshall

2009-01-01

Distribution of sex-determining mechanisms across Australian agamids shows no clear phylogenetic segregation, suggesting multiple transitions between temperature-dependent (TSD) and genotypic sex determination (GSD). These taxa thus present an excellent opportunity for studying the evolution of sex chromosomes, and evolutionary transitions between TSD and GSD. Here we report the hybridization of a 3 kb genomic sequence (PvZW3) that marks the Z and W microchromosomes of the Australian central bearded dragon (Pogona vitticeps) to chromosomes of 12 species of Australian agamids from eight genera using fluorescence in-situ hybridization (FISH). The probe hybridized to a single microchromosome pair in 11 of these species, but to the tip of the long arm of chromosome pair 2 in the twelfth (Physignathus lesueurii), indicating a micro-macro chromosome rearrangement. Three TSD species shared the marked microchromosome, implying that it is a conserved autosome in related species that determine sex by temperature. C-banding identified the marked microchromosome as the heterochromatic W chromosome in two of the three GSD species. However, in Ctenophorus fordi, the probe hybridized to a different microchromosome from that shown by C-banding to be the heterochromatic W, suggesting an independent origin for the ZW chromosome pair in that species. Given the haphazard distribution of GSD and TSD in this group and the existence of at least two sets of sex microchromosomes in GSD species, we conclude that sex-determining mechanisms in this family have evolved independently, multiple times in a short evolutionary period.

Evidence for the absence of cerebral glucose-6-phosphatase activity in glycogen storage disease type I (Von Gierke's disease)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Phelps, M.E.; Mazziotta, J.C.; Hawkins, R.A.

1981-01-01

Glycogen storage disease type I (GSD-I) is characterized by a functional deficit in glucose-6-phosphatase that normally hydrolyzes glucose-6-PO/sub 4/ to glucose. This enzyme is primarily found in liver, kidney, and muscle but it is also present in brain, where it appears to participate in the regulation of cerebral tissue glucose. Since most neurological symptoms in GSD-I patients involve systemic hypoglycemia, previous reports have not examined possible deficiencies in phosphatase activity in the brain. Positron computed tomography, F-18-labeled 2-fluorodeoxyglucose (FDG) and a tracer kinetic model for FDG were used to measure the cortical plasma/tissue forward and reverse transport, phosphorylation and dephosphorylationmore » rate constants, tissue/plasma concentration gradient, tissue concentration turnover rate for this competitive analog of glucose, and the cortical metabolic rates for glucose. Studies were carried out in age-matched normals (N = 13) and a single GSD-I patient. The dephosphorylation rate constant in the GSD-I patient was about one tenth the normal value indicating a low level of cerebral phosphatase activity. The other measured parameters were within normal limits except for the rate of glucose phosphorylation which reflected a cortical glucose metabolic rate one half the normal value. Since glucose transport and tissue glucose concentration was normal, the reduced cortical glucose metabolism probably results from the use of alternative substrates (..beta..-hydroxybutyrate and acetoacetate) which are consistently elevated in the plasma of GSD-I patients.« less

What was the ancestral sex-determining mechanism in amniote vertebrates?

PubMed

Johnson Pokorná, Martina; Kratochvíl, Lukáš

2016-02-01

Amniote vertebrates, the group consisting of mammals and reptiles including birds, possess various mechanisms of sex determination. Under environmental sex determination (ESD), the sex of individuals depends on the environmental conditions occurring during their development and therefore there are no sexual differences present in their genotypes. Alternatively, through the mode of genotypic sex determination (GSD), sex is determined by a sex-specific genotype, i.e. by the combination of sex chromosomes at various stages of differentiation at conception. As well as influencing sex determination, sex-specific parts of genomes may, and often do, develop specific reproductive or ecological roles in their bearers. Accordingly, an individual with a mismatch between phenotypic (gonadal) and genotypic sex, for example an individual sex-reversed by environmental effects, should have a lower fitness due to the lack of specialized, sex-specific parts of their genome. In this case, evolutionary transitions from GSD to ESD should be less likely than transitions in the opposite direction. This prediction contrasts with the view that GSD was the ancestral sex-determining mechanism for amniote vertebrates. Ancestral GSD would require several transitions from GSD to ESD associated with an independent dedifferentiation of sex chromosomes, at least in the ancestors of crocodiles, turtles, and lepidosaurs (tuataras and squamate reptiles). In this review, we argue that the alternative theory postulating ESD as ancestral in amniotes is more parsimonious and is largely concordant with the theoretical expectations and current knowledge of the phylogenetic distribution and homology of sex-determining mechanisms. © 2014 Cambridge Philosophical Society.

Discovery of the youngest sex chromosomes reveals first case of convergent co-option of ancestral autosomes in turtles.

PubMed

Montiel, E E; Badenhorst, D; Tamplin, J; Burke, R L; Valenzuela, N

2017-02-01

Most turtle species possess temperature-dependent sex determination (TSD), but genotypic sex determination (GSD) has evolved multiple times independently from the TSD ancestral condition. GSD in animals typically involves sex chromosomes, yet the sex chromosome system of only 9 out of 18 known GSD turtles has been characterized. Here, we combine comparative genome hybridization (CGH) and BAC clone fluorescent in situ hybridization (BAC FISH) to identify a macro-chromosome XX/XY system in the GSD wood turtle Glyptemys insculpta (GIN), the youngest known sex chromosomes in chelonians (8-20 My old). Comparative analyses show that GIN-X/Y is homologous to chromosome 4 of Chrysemys picta (CPI) painted turtles, chromosome 5 of Gallus gallus chicken, and thus to the X/Y sex chromosomes of Siebenrockiella crassicollis black marsh turtles. We tentatively assign the gene content of the mapped BACs from CPI chromosome 4 (CPI-4) to GIN-X/Y. Chromosomal rearrangements were detected in G. insculpta sex chromosome pair that co-localize with the male-specific region of GIN-Y and encompass a gene involved in sexual development (Wt1-a putative master gene in TSD turtles). Such inversions may have mediated the divergence of G. insculpta sex chromosome pair and facilitated GSD evolution in this turtle. Our results illuminate the structure, origin, and evolution of sex chromosomes in G. insculpta and reveal the first case of convergent co-option of an autosomal pair as sex chromosomes within chelonians.

Genetic Basis of Glycogen Storage Disease Type 1a: Prevalent Mutations at the Glucose-6-Phosphatase Locus

PubMed Central

Lei, Ke-Jian; Chen, Yuan-Tsong; Chen, Hungwen; Wong, Lee-Jun C.; Liu, Ji-Lan; McConkie-Rosell, Allyn; Van Hove, Johan L. K.; Ou, Henry C.-Y.; Yeh, Nan Jung; Pan, Lorraine Y.; Chou, Janice Yang

1995-01-01

Diagnosis of glycogen storage disease (GSD) type 1a currently is established by demonstrating the lack of glucose-6-phosphatase (G6Pase) activity in the patient's biopsied liver specimen. Recent cloning of the G6Pase gene and identification of mutations within the gene that causes GSD type 1a allow for the development of a DNA-based diagnostic method. Using SSCP analysis and DNA sequencing, we characterized the G6Pase gene of 70 unrelated patients with enzymatically confirmed diagnosis of GSD type 1a and detected mutations in all except 17 alleles (88%). Sixteen mutations were uncovered that were shown by expression to abolish or greatly reduce G6Pase activity and that therefore are responsible for the GSD type 1a disorder. R83C and Q347X are the most prevalent mutations found in Caucasians, 130X and R83C are most prevalent in Hispanics, and R83H is most prevalent in Chinese. The Q347X mutation has thus far been identified only in Caucasian patients, and the 130X mutation has been identified only in Hispanic patients. Our results demonstrate that the DNA-based analysis can accurately, rapidly, and noninvasively detect the majority of mutations in GSD type 1a. This DNA-based diagnosis now permits prenatal diagnosis among at-risk patients and serves as a database in screening and counseling patients clinically suspected of having this disease. ImagesFigure 1Figure 2 PMID:7573034

Assessment of road users' elemental carbon personal exposure levels, London, UK

NASA Astrophysics Data System (ADS)

Adams, H. S.; Nieuwenhuijsen, M. J.; Colvile, R. N.; Older, M. J.; Kendall, M.

Little is known about particulate elemental carbon (EC) personal exposure levels, a key component of diesel exhaust, specifically in transport microenvironments. A method utilizing the optical properties of EC particles has been applied to personal exposure measurement filter samples. In a series of field studies carried out in London, UK, during 1999-2000 over 400 fine particle (PM 2.5) personal exposure level measurements were taken for journeys in bicycle, bus, car and underground rail transport microenvironments, along three main fixed routes. The particulate EC contribution to the PM 2.5 personal exposure was assessed indirectly by means of an optical technique and with the development and use of a size fraction specific and site-specific calibration curve. In this first EC personal exposure study of transport users geometric mean exposure levels in the summer field campaign were 11.2 μg m -3 (GSD=2.7) for cyclists, 13.6 μg m -3 (GSD=1.9) for bus passengers and 21.6 μg m -3 (GSD=2.1) for car drivers; corresponding exposure levels in the winter were 16.4 μg m -3 (GSD=1.8), 18.6 μg m -3 (GSD=2.3) and 27.3 μg m -3 (GSD=2.0), respectively. EC/PM 2.5 ratios were approximately 0.5-0.6 for bicycle and bus modes and 0.7-0.8 for the car mode. EC/PM 2.5 ratios for different routes ranged from approximately 0.7 for Route 1 to 0.4 for Route 3. Cyclists had the lowest exposure to EC, and car occupants the highest exposure. A large difference in exposure levels between a central high traffic density route and the other less central routes was observed. Particulate EC was a very significant proportion of the total PM 2.5 personal exposure and EC personal exposure levels were considerably higher than reported fixed site monitor EC concentrations.

Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.

PubMed

Wang, Wensheng; Wang, Hua; Zhou, Xichao; Li, Xing; Sun, Wen; Dellinger, Michael; Boyce, Brendan F; Xing, Lianping

2017-05-01

Gorham-Stout disease (GSD) is a rare bone disorder characterized by aggressive osteolysis associated with lymphatic vessel invasion within bone marrow cavities. The etiology of GSD is not known, and there is no effective therapy or animal model for the disease. Here, we investigated if lymphatic endothelial cells (LECs) affect osteoclasts (OCs) to cause a GSD osteolytic phenotype in mice. We examined the effect of a mouse LEC line on osteoclastogenesis in co-cultures. LECs significantly increased receptor activator of NF-κB ligand (RANKL)-mediated OC formation and bone resorption. LECs expressed high levels of macrophage colony-stimulating factor (M-CSF), but not RANKL, interleukin-6 (IL-6), and tumor necrosis factor (TNF). LEC-mediated OC formation and bone resorption were blocked by an M-CSF neutralizing antibody or Ki20227, an inhibitor of the M-CSF receptor, c-Fms. We injected LECs into the tibias of wild-type (WT) mice and observed massive osteolysis on X-ray and micro-CT scans. Histology showed that LEC-injected tibias had significant trabecular and cortical bone loss and increased OC numbers. M-CSF protein levels were significantly higher in serum and bone marrow plasma of mice given intra-tibial LEC injections. Immunofluorescence staining showed extensive replacement of bone and marrow by podoplanin+ LECs. Treatment of LEC-injected mice with Ki20227 significantly decreased tibial bone destruction. In addition, lymphatic vessels in a GSD bone sample were stained positively for M-CSF. Thus, LECs cause bone destruction in vivo in mice by secreting M-CSF, which promotes OC formation and activation. Blocking M-CSF signaling may represent a new therapeutic approach for treatment of patients with GSD. Furthermore, tibial injection of LECs is a useful mouse model to study GSD. © 2017 American Society for Bone and Mineral Research. © 2017 American Society for Bone and Mineral Research.

Cigarettes vs. e-cigarettes: Passive exposure at home measured by means of airborne marker and biomarkers.

PubMed

Ballbè, Montse; Martínez-Sánchez, Jose M; Sureda, Xisca; Fu, Marcela; Pérez-Ortuño, Raúl; Pascual, José A; Saltó, Esteve; Fernández, Esteve

2014-11-01

There is scarce evidence about passive exposure to the vapour released or exhaled from electronic cigarettes (e-cigarettes) under real conditions. The aim of this study is to characterise passive exposure to nicotine from e-cigarettes' vapour and conventional cigarettes' smoke at home among non-smokers under real-use conditions. We conducted an observational study with 54 non-smoker volunteers from different homes: 25 living at home with conventional smokers, 5 living with nicotine e-cigarette users, and 24 from control homes (not using conventional cigarettes neither e-cigarettes). We measured airborne nicotine at home and biomarkers (cotinine in saliva and urine). We calculated geometric mean (GM) and geometric standard deviations (GSD). We also performed ANOVA and Student's t tests for the log-transformed data. We used Bonferroni-corrected t-tests to control the family error rate for multiple comparisons at 5%. The GMs of airborne nicotine were 0.74 μg/m(3) (GSD=4.05) in the smokers' homes, 0.13 μg/m(3) (GSD=2.4) in the e-cigarettes users' homes, and 0.02 μg/m(3) (GSD=3.51) in the control homes. The GMs of salivary cotinine were 0.38 ng/ml (GSD=2.34) in the smokers' homes, 0.19 ng/ml (GSD=2.17) in the e-cigarettes users' homes, and 0.07 ng/ml (GSD=1.79) in the control homes. Salivary cotinine concentrations of the non-smokers exposed to e-cigarette's vapour at home (all exposed ≥ 2 h/day) were statistically significant different that those found in non-smokers exposed to second-hand smoke ≥ 2 h/day and in non-smokers from control homes. The airborne markers were statistically higher in conventional cigarette homes than in e-cigarettes homes (5.7 times higher). However, concentrations of both biomarkers among non-smokers exposed to conventional cigarettes and e-cigarettes' vapour were statistically similar (only 2 and 1.4 times higher, respectively). The levels of airborne nicotine and cotinine concentrations in the homes with e-cigarette users were higher than control homes (differences statistically significant). Our results show that non-smokers passively exposed to e-cigarettes absorb nicotine. Copyright © 2014. Published by Elsevier Inc.

GSD Update: Restoration on the range: Healing America's iconic landscapes

Treesearch

Christie Aschwanden

2011-01-01

Restoration is one of five focal areas of the Grassland, Shrubland and Desert Ecosystems Science Program (GSD). Our program mission is to develop and deliver knowledge and tools that will help to sustain and restore grasslands, shrublands and deserts threatened by invasive species, disturbances, urban pressures and climate change. Scientists in our program develop...

Genetic basis of glycogen storage disease type 1a: Prevalent mutations at the glucose-6-phosphatase locus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ke-Jian Lei; Hungwen Chen; Ji-Lan Liu

Diagnosis of glycogen storage disease (GSD) type 1a currently is established by demonstrating the lack of glucose-6-phosphatase (G6Pase) activity in the patient`s biopsied liver specimen. Recent cloning of the G6Pase gene and identification of mutations within the gene that causes GSD type 1a allow for the development of a DNA-based diagnostic method. Using SSCP analysis and DNA sequencing, we characterized the G6Pase gene of 70 unrelated patients with enzymatically confirmed diagnosis of GSD type 1a and detected mutations in all except 17 alleles (88%). Sixteen mutations were uncovered that were shown by expression to abolish or greatly reduce G6Pase activitymore » and that therefore are responsible for the GSD type la disorder. R83C and Q347X are the most prevalent mutations found in Caucasians, 130X and R83C are most prevalent in Hispanics, and R83H is most prevalent in Chinese. The Q347X mutation has thus far been identified only in Caucasian patients, and the 130X mutation has been identified only in Hispanic patients. Our results demonstrate that the DNA-based analysis can accurately, rapidly, and noninvasively detect the majority of mutations in GSD type 1a. This DNA-based diagnosis now permits prenatal diagnosis among at-risk patients and serves as a database in screening and counseling patients clinically suspected of having this disease. 22 refs., 2 figs., 4 tabs.« less

[Molecular and clinical characterization of Colombian patients suffering from type III glycogen storage disease].

PubMed

Mantilla, Carolina; Toro, Mónica; Sepúlveda, María Elsy; Insuasty, Margarita; Di Filippo, Diana; López, Juan Álvaro; Baquero, Carolina; Navas, María Cristina; Arias, Andrés Augusto

2018-05-01

Type III glycogen storage disease (GSD III) is an autosomal recessive disorder in which a mutation in the AGL gene causes deficiency of the glycogen debranching enzyme. The disease is characterized by fasting hypoglycemia, hepatomegaly and progressive myopathy. Molecular analyses of AGL have indicated heterogeneity depending on ethnic groups. The full spectrum of AGL mutations in Colombia remains unclear. To describe the clinical and molecular characteristics of ten Colombian patients diagnosed with GSD III. We recruited ten Colombian children with a clinical and biochemical diagnosis of GSD III to undergo genetic testing. The full coding exons and the relevant exon-intron boundaries of the AGL underwent Sanger sequencing to identify mutation. All patients had the classic phenotype of the GSD III. Genetic analysis revealed a mutation p.Arg910X in two patients. One patient had the mutation p.Glu1072AspfsX36, and one case showed a compound heterozygosity with p.Arg910X and p.Glu1072AspfsX36 mutations. We also detected the deletion of AGL gene 3, 4, 5, and 6 exons in three patients. The in silico studies predicted that these defects are pathogenic. No mutations were detected in the amplified regions in three patients. We found mutations and deletions that explain the clinical phenotype of GSD III patients. This is the first report with a description of the clinical phenotype and the spectrum of AGL mutations in Colombian patients. This is important to provide appropriate prognosis and genetic counseling to the patient and their relatives.

Grain-size analysis of volcanic ash for the rapid assessment of respiratory health hazard.

PubMed

Horwell, Claire J

2007-10-01

Volcanic ash has the potential to cause acute and chronic respiratory diseases if the particles are sufficiently fine to enter the respiratory system. Characterization of the grain-size distribution (GSD) of volcanic ash is, therefore, a critical first step in assessing its health hazard. Quantification of health-relevant size fractions is challenging without state-of-the-art technology, such as the laser diffractometer. Here, several methods for GSD characterization for health assessment are considered, the potential for low-cost measurements is investigated and the first database of health-pertinent GSD data is presented for a suite of ash samples from around the world. Methodologies for accurate measurement of the GSD of volcanic ash by laser diffraction are presented by experimental analysis of optimal refractive indices for different magmatic compositions. Techniques for representative sampling of small quantities of ash are also experimentally investigated. GSD results for health-pertinent fractions for a suite of 63 ash samples show that the fraction of respirable (<4 microm) material ranges from 0-17 vol%, with the variation reflecting factors such as the style of the eruption and the distance from the source. A strong correlation between the amount of <4 and <10 microm material is observed for all ash types. This relationship is stable at all distances from the volcano and with all eruption styles and can be applied to volcanic plume and ash fallout models. A weaker relationship between the <4 and <63 microm fractions provides a novel means of estimating the quantity of respirable material from data obtained by sieving.

Bariatric surgery is not contraindicated in obese patients suffering from glycogen storage disease type IXa. A case report with follow-up at three years.

PubMed

Musella, Mario; Milone, Marco; Maietta, Paola; Bianco, Paolo; Pisapia, Anna; Gaudioso, Dario; Palumbo, Rubina

2014-01-01

Glucose storage disease type IXa (GSD IXa) is an uncommon condition presenting with childhood onset hepatomegaly, growth retardation, and often, fasting ketosis and hypoglycemia. Despite its benign course, the lack of dietary counseling may favor uncontrolled weight gain. We investigated the efficacy of bariatric surgery in one 17 years old female suffering from GSD IXa and morbid obesity. The diagnosis was GSD type IXa in a patient with a body mass index (BMI) of 45.5kg/m(2). Onset of hypoglycemia was reported twice each month. She was treated her implanting an adjustable gastric banding through laparoscopy. Three years after surgery the patient presents a BMI of 30.1kg/m(2) and an excess of weight loss (EWL) of 71.1%. Only once, following surgery, she had to deflate her band to allow a faster transit of food through her stomach, thus reaching a prompt euglycemic condition, due to an incoming hypoglycemic crisis. Laparoscopic adjustable gastric banding (LAGB) is one of the most used approaches to treat morbid obesity. It is a restrictive procedure unable to affect the absorption of any nutrient, presenting a very low intra and perioperative complication rate. In our GSD IXa patient, it offered a prompt modification of food intake restriction whenever requested, thus avoiding hypoglycemia. LAGB is effective in determining weight loss without inducing significant side effects or worsening hypoglycemia, in this morbid obese patient, suffering from GSD type IXa. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

Preclinical Development of New Therapy for Glycogen Storage Diseases

PubMed Central

Sun, Baodong; Brooks, Elizabeth D.; Koeberl, Dwight D.

2015-01-01

Glycogen storage disease (GSD) consists of more than 10 discrete conditions for which the biochemical and genetic bases have been determined, and new therapies have been under development for several of these conditions. Gene therapy research has generated proof-of-concept for GSD types I (von Gierke disease) and II (Pompe disease). Key features of these gene therapy strategies include the choice of vector and regulatory cassette, and recently adeno-associated virus (AAV) vectors containing tissue-specific promoters have achieved a high degree of efficacy. Efficacy of gene therapy for Pompe disease depend upon the induction of immune tolerance to the therapeutic enzyme. Efficacy of von Gierke disease is transient, waning gradually over the months following vector administration. Small molecule therapies have been evaluated with the goal of improving standard of care therapy or ameliorating the cellular abnormalities associated with specific GSDs. The receptor-mediated uptake of the therapeutic enzyme in Pompe disease was enhanced by administration of β2 agonists. Rapamycin reduced the liver fibrosis observed in GSD III. Further development of gene therapy could provide curative therapy for patients with GSD, if efficacy from preclinical research is observed in future clinical trials and these treatments become clinically available. PMID:26122079

Software project management tools in global software development: a systematic mapping study.

PubMed

Chadli, Saad Yasser; Idri, Ali; Ros, Joaquín Nicolás; Fernández-Alemán, José Luis; de Gea, Juan M Carrillo; Toval, Ambrosio

2016-01-01

Global software development (GSD) which is a growing trend in the software industry is characterized by a highly distributed environment. Performing software project management (SPM) in such conditions implies the need to overcome new limitations resulting from cultural, temporal and geographic separation. The aim of this research is to discover and classify the various tools mentioned in literature that provide GSD project managers with support and to identify in what way they support group interaction. A systematic mapping study has been performed by means of automatic searches in five sources. We have then synthesized the data extracted and presented the results of this study. A total of 102 tools were identified as being used in SPM activities in GSD. We have classified these tools, according to the software life cycle process on which they focus and how they support the 3C collaboration model (communication, coordination and cooperation). The majority of the tools found are standalone tools (77%). A small number of platforms (8%) also offer a set of interacting tools that cover the software development lifecycle. Results also indicate that SPM areas in GSD are not adequately supported by corresponding tools and deserve more attention from tool builders.

Numerical models of caldera deformation: Effects of multiphase and multicomponent hydrothermal fluid flow

USGS Publications Warehouse

Hutnak, M.; Hurwitz, S.; Ingebritsen, S.E.; Hsieh, P.A.

2009-01-01

Ground surface displacement (GSD) in large calderas is often interpreted as resulting from magma intrusion at depth. Recent advances in geodetic measurements of GSD, notably interferometric synthetic aperture radar, reveal complex and multifaceted deformation patterns that often require complex source models to explain the observed GSD. Although hydrothermal fluids have been discussed as a possible deformation agent, very few quantitative studies addressing the effects of multiphase flow on crustal mechanics have been attempted. Recent increases in the power and availability of computing resources allow robust quantitative assessment of the complex time-variant thermal interplay between aqueous fluid flow and crustal deformation. We carry out numerical simulations of multiphase (liquid-gas), multicomponent (H 2O-CO2) hydrothermal fluid flow and poroelastic deformation using a range of realistic physical parameters and processes. Hydrothermal fluid injection, circulation, and gas formation can generate complex, temporally and spatially varying patterns of GSD, with deformation rates, magnitudes, and geometries (including subsidence) similar to those observed in several large calderas. The potential for both rapid and gradual deformation resulting from magma-derived fluids suggests that hydrothermal fluid circulation may help explain deformation episodes at calderas that have not culminated in magmatic eruption.

Cutting Edge: Increased Autoimmunity Risk in Glycogen Storage Disease Type 1b Is Associated with a Reduced Engagement of Glycolysis in T Cells and an Impaired Regulatory T Cell Function.

PubMed

Melis, Daniela; Carbone, Fortunata; Minopoli, Giorgia; La Rocca, Claudia; Perna, Francesco; De Rosa, Veronica; Galgani, Mario; Andria, Generoso; Parenti, Giancarlo; Matarese, Giuseppe

2017-05-15

Glycogen storage disease type 1b (GSD-1b) is an autosomal-recessive disease caused by mutation of glucose-6-phosphate transporter and characterized by altered glycogen/glucose homeostasis. A higher frequency of autoimmune diseases has been observed in GSD-1b patients, but the molecular determinants leading to this phenomenon remain unknown. To address this question, we investigated the effect of glucose-6-phosphate transporter mutation on immune cell homeostasis and CD4 + T cell functions. In GSD-1b subjects, we found lymphopenia and a reduced capacity of T cells to engage glycolysis upon TCR stimulation. These phenomena associated with reduced expression of the FOXP3 transcription factor, lower suppressive function in peripheral CD4 + CD25 + FOXP3 + regulatory T cells, and an impaired capacity of CD4 + CD25 - conventional T cells to induce expression of FOXP3 after suboptimal TCR stimulation. These data unveil the metabolic determinant leading to an increased autoimmunity risk in GSD-1b patients. Copyright © 2017 by The American Association of Immunologists, Inc.

Comparative study of quality characteristics of Korean soy sauce made with soybeans germinated under dark and light conditions.

PubMed

Choi, Ung-Kyu; Jeong, Yeon-Shin; Kwon, O-Jun; Park, Jong-Dae; Kim, Young-Chan

2011-01-01

This study was conducted to evaluate the effects of germinating soybeans under dark and light conditions on the quality characteristics of Korean soy sauce made with germinated soybeans. The germination rate of soybeans germinated under dark conditions (GSD) was higher than that of soybeans germinated under light conditions (GSL), whereas the lengths of sprouts and relative weights of GSL did not differ from those of GSD. The L, a, b, and ΔT values of GSL were significantly lower than GSD. The color of GSD remained yellow, while GSL changed to a green color due to photosynthesis by chlorophyll. The total amino acid contents in soy sauce fermented with soybeans germinated under dark conditions (SSGD) and soy sauce fermented with soybeans germinated under light conditions (SSGL) were lower than in soy sauce fermented with non-germinated soybeans (SNGS). The levels of isoflavone content in SSGD and SSGL were significantly increased compared to the SNGS. In conclusion, the germination of soybeans under dark and light conditions is not only an increasing organoleptic preference, but also has implications for the health benefits of Korean soy sauce.

Two new mutations in the glucose-6-phosphatase gene cause glycogen storage disease in Hungarian patients.

PubMed

Parvari, R; Lei, K J; Szonyi, L; Narkis, G; Moses, S; Chou, J Y

1997-01-01

Glycogen storage disease type 1a (von Gierke disease, GSD-1A) is caused by the deficiency of microsomal glucose-6-phosphatase (G6Pase) activity which catalyzes the final common step of glycogenolysis and gluconeogenesis. The cloning of the G6Pase cDNA and characterization of the human G6Pase gene enabled the identification of the mutations causing GSD-1a. This, in turn, allows the development of non-invasive DNA-based diagnosis that provides reliable carrier testing and prenatal diagnosis. Here we report on two new mutations E110Q and D38V causing GSD-1a in two Hungarian patients. The analyses of these mutations by site-directed mutagenesis followed by transient expression assays demonstrated that E110Q retains 17% of G6Pase enzymatic activity while the D38V abolishes the enzymatic activity. The patient with the E110Q has G222R as his other mutation. G222R was also shown to preserve about 4% of the G6Pase enzymatic activity. Nevertheless, the patient presented with the classical severe symptomatology of the GSD-1a.

Comparative Study of Quality Characteristics of Korean Soy Sauce Made with Soybeans Germinated Under Dark and Light Conditions

PubMed Central

Choi, Ung-Kyu; Jeong, Yeon-Shin; Kwon, O-Jun; Park, Jong-Dae; Kim, Young-Chan

2011-01-01

This study was conducted to evaluate the effects of germinating soybeans under dark and light conditions on the quality characteristics of Korean soy sauce made with germinated soybeans. The germination rate of soybeans germinated under dark conditions (GSD) was higher than that of soybeans germinated under light conditions (GSL), whereas the lengths of sprouts and relative weights of GSL did not differ from those of GSD. The L, a, b, and ΔT values of GSL were significantly lower than GSD. The color of GSD remained yellow, while GSL changed to a green color due to photosynthesis by chlorophyll. The total amino acid contents in soy sauce fermented with soybeans germinated under dark conditions (SSGD) and soy sauce fermented with soybeans germinated under light conditions (SSGL) were lower than in soy sauce fermented with non-germinated soybeans (SNGS). The levels of isoflavone content in SSGD and SSGL were significantly increased compared to the SNGS. In conclusion, the germination of soybeans under dark and light conditions is not only an increasing organoleptic preference, but also has implications for the health benefits of Korean soy sauce. PMID:22174653

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robison, W L; Hamilton, T F; Bogen, K

Inter-plant concentration ratios (IPCR), [Bq g{sup -1} {sup 137}Cs in coral atoll tree food-crops/Bq g{sup -1} {sup 137}Cs in leaves of native plant species whose roots share a common soil volume], can replace transfer factors (TF) to predict {sup 137}Cs concentration in tree food-crops in a contaminated area with an aged source term. The IPCR strategy has significant benefits relative to TF strategy for such purposes in the atoll ecosystem. IPCR strategy applied to specific assessments takes advantage of the fact tree roots naturally integrate 137Cs over large volumes of soil. Root absorption of {sup 137}Cs replaces large-scale, expensive soilmore » sampling schemes to reduce variability in {sup 137}Cs concentration due to inhomogeneous radionuclide distribution. IPCR [drinking-coconut meat (DCM)/Scaevola (SCA) and Tournefortia (TOU) leaves (native trees growing on all atoll islands)] are log normally distributed (LND) with geometric standard deviation (GSD) = 1.85. TF for DCM from Enewetak, Eneu, Rongelap and Bikini Atolls are LND with GSD's of 3.5, 3.0, 2.7, and 2.1, respectively. TF GSD for Rongelap copra coconut meat is 2.5. IPCR of Pandanus fruit to SCA and TOU leaves are LND with GSD = 1.7 while TF GSD is 2.1. Because IPCR variability is much lower than TF variability, relative sampling error of an IPCR field sample mean is up 6- to 10-fold lower than that of a TF sample mean if sample sizes are small (10 to 20). Other IPCR advantages are that plant leaf samples are collected and processed in far less time with much less effort and cost than soil samples.« less

Effects on cognitive and clinical insight with the use of Guided Self-Determination in outpatients with schizophrenia: A randomized open trial.

PubMed

Jørgensen, R; Licht, R W; Lysaker, P H; Munk-Jørgensen, P; Buck, K D; Jensen, S O W; Hansson, L; Zoffmann, V

2015-07-01

Poor insight has a negative impact on the outcome in schizophrenia; consequently, poor insight is a logical target for treatment. However, neither medication nor psychosocial interventions have been demonstrated to improve poor insight. A method originally designed for diabetes patients to improve their illness management, Guided Self-Determination (GSD), has been adapted for use in patients with schizophrenia (GSD-SZ). The purpose of this study was to investigate the effect on insight of GSD-SZ as a supplement to treatment as usual (TAU) as compared to TAU alone in outpatients diagnosed with schizophrenia. The design was an open randomized trial. The primary hypothesis was cognitive insight would improve in those patients who received GSD-SZ+TAU as assessed by the BCIS. We additionally explored whether the intervention led to changes in clinical insight, self-perceived recovery, self-esteem, social functioning and symptom severity. Assessments were conducted at baseline, and at 3-, 6- and 12-month follow-up. Analysis was based on the principles of intention to treat and potential confounders were taken into account through applying a multivariate approach. A total of 101 participants were randomized to GSD-SZ+TAU (n=50) or to TAU alone (n=51). No statistically significant differences were found on the cognitive insight. However, at 12-month follow-up, clinical insight (measured by G12 from the Positive and Negative Syndrome Scale), symptom severity, and social functioning had statistically significantly improved in the intervention group as compared to the control group. "Improving insight in patients diagnosed with schizophrenia", NCT01282307, http://clinicaltrials.gov/. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

Effect of roll compaction on granule size distribution of microcrystalline cellulose–mannitol mixtures: computational intelligence modeling and parametric analysis

PubMed Central

Kazemi, Pezhman; Khalid, Mohammad Hassan; Pérez Gago, Ana; Kleinebudde, Peter; Jachowicz, Renata; Szlęk, Jakub; Mendyk, Aleksander

2017-01-01

Dry granulation using roll compaction is a typical unit operation for producing solid dosage forms in the pharmaceutical industry. Dry granulation is commonly used if the powder mixture is sensitive to heat and moisture and has poor flow properties. The output of roll compaction is compacted ribbons that exhibit different properties based on the adjusted process parameters. These ribbons are then milled into granules and finally compressed into tablets. The properties of the ribbons directly affect the granule size distribution (GSD) and the quality of final products; thus, it is imperative to study the effect of roll compaction process parameters on GSD. The understanding of how the roll compactor process parameters and material properties interact with each other will allow accurate control of the process, leading to the implementation of quality by design practices. Computational intelligence (CI) methods have a great potential for being used within the scope of quality by design approach. The main objective of this study was to show how the computational intelligence techniques can be useful to predict the GSD by using different process conditions of roll compaction and material properties. Different techniques such as multiple linear regression, artificial neural networks, random forest, Cubist and k-nearest neighbors algorithm assisted by sevenfold cross-validation were used to present generalized models for the prediction of GSD based on roll compaction process setting and material properties. The normalized root-mean-squared error and the coefficient of determination (R2) were used for model assessment. The best fit was obtained by Cubist model (normalized root-mean-squared error =3.22%, R2=0.95). Based on the results, it was confirmed that the material properties (true density) followed by compaction force have the most significant effect on GSD. PMID:28176905

Effect of roll compaction on granule size distribution of microcrystalline cellulose-mannitol mixtures: computational intelligence modeling and parametric analysis.

PubMed

Kazemi, Pezhman; Khalid, Mohammad Hassan; Pérez Gago, Ana; Kleinebudde, Peter; Jachowicz, Renata; Szlęk, Jakub; Mendyk, Aleksander

2017-01-01

Dry granulation using roll compaction is a typical unit operation for producing solid dosage forms in the pharmaceutical industry. Dry granulation is commonly used if the powder mixture is sensitive to heat and moisture and has poor flow properties. The output of roll compaction is compacted ribbons that exhibit different properties based on the adjusted process parameters. These ribbons are then milled into granules and finally compressed into tablets. The properties of the ribbons directly affect the granule size distribution (GSD) and the quality of final products; thus, it is imperative to study the effect of roll compaction process parameters on GSD. The understanding of how the roll compactor process parameters and material properties interact with each other will allow accurate control of the process, leading to the implementation of quality by design practices. Computational intelligence (CI) methods have a great potential for being used within the scope of quality by design approach. The main objective of this study was to show how the computational intelligence techniques can be useful to predict the GSD by using different process conditions of roll compaction and material properties. Different techniques such as multiple linear regression, artificial neural networks, random forest, Cubist and k-nearest neighbors algorithm assisted by sevenfold cross-validation were used to present generalized models for the prediction of GSD based on roll compaction process setting and material properties. The normalized root-mean-squared error and the coefficient of determination ( R 2 ) were used for model assessment. The best fit was obtained by Cubist model (normalized root-mean-squared error =3.22%, R 2 =0.95). Based on the results, it was confirmed that the material properties (true density) followed by compaction force have the most significant effect on GSD.

Bleeding pattern and cycle control of a low-dose transdermal contraceptive patch compared with a combined oral contraceptive: a randomized study.

PubMed

Merz, M; Kroll, R; Lynen, R; Bangerter, K

2015-02-01

The aim of this study was to investigate the bleeding pattern and cycle control of a contraceptive patch containing 0.55 mg ethinyl estradiol (EE) and 2.1 mg gestodene (GSD) compared with a combined oral contraceptive (COC) containing 0.02 mg EE and 0.1 mg levonorgestrel (LNG). In this phase III, randomized, controlled, double-blind, double-dummy, multicenter trial, healthy women aged 18-45 years (smokers aged 18-35 years) received either the EE/GSD patch and a placebo tablet (n=171), or a placebo patch and the COC (n=175) for seven 28-day cycles. Bleeding control was assessed in two 90-day reference periods. Mean number of bleeding/spotting days was comparable across treatment groups in both reference periods (p>.05). Mean number of bleeding/spotting episodes was also comparable in reference period 1; however, there were fewer bleeding/spotting episodes for COC in reference period 2 (3.4 versus 3.1; p=.01). Mean length of bleeding/spotting episodes was comparable across treatment groups for both reference periods (p>.05). Withdrawal bleeding occurred consistently in both groups over the entire treatment period, but its absence was more common in the COC group in cycles 4 and 6 of reference period 2 (p<.01). Intracyclic bleeding was comparable between groups. Bleeding pattern and cycle control with the EE/GSD patch was comparable to an EE/LNG-containing COC. The findings suggest that bleeding patterns with the EE/GSD patch are similar to an EE/LNG-containing COC, except for absence of withdrawal bleeding, which was less common in patch users. The EE/GSD patch may constitute an additional contraceptive option for women. Copyright © 2015 Elsevier Inc. All rights reserved.

ESTIMATING THE ECONOMIC VALUE OF NATIONAL PARKS WITH COUNT DATA MODELS USING ON-SITE, SECONDARY DATA: THE CASE OF THE GREAT SAND DUNES NATIONAL PARK AND PRESERVE

EPA Science Inventory

We estimate an individual travel cost model for Great Sand Dunes National Park and Preserve (GSD) in Colorado using on-site, secondary data. The purpose of the on-site survey was to help the National Park Service better understand the visitors of GSD; it was not intended for a t...

GSD-1G and MPI-DING Reference Glasses for In Situ and Bulk Isotopic Determination

USGS Publications Warehouse

Jochum, K.P.; Wilson, S.A.; Abouchami, W.; Amini, M.; Chmeleff, J.; Eisenhauer, A.; Hegner, E.; Iaccheri, L.M.; Kieffer, B.; Krause, J.; McDonough, W.F.; Mertz-Kraus, R.; Raczek, I.; Rudnick, R.L.; Scholz, Donna K.; Steinhoefel, G.; Stoll, B.; Stracke, A.; Tonarini, S.; Weis, D.; Weis, U.; Woodhead, J.D.

2011-01-01

This paper contains the results of an extensive isotopic study of United States Geological Survey GSD-1G and MPI-DING reference glasses. Thirteen different laboratories were involved using high-precision bulk (TIMS, MC-ICP-MS) and microanalytical (LA-MC-ICP-MS, LA-ICP-MS) techniques. Detailed studies were performed to demonstrate the large-scale and small-scale homogeneity of the reference glasses. Together with previously published isotopic data from ten other laboratories, preliminary reference and information values as well as their uncertainties at the 95% confidence level were determined for H, O, Li, B, Si, Ca, Sr, Nd, Hf, Pb, Th and U isotopes using the recommendations of the International Association of Geoanalysts for certification of reference materials. Our results indicate that GSD-1G and the MPI-DING glasses are suitable reference materials for microanalytical and bulk analytical purposes. Ce document contient les r??sultats d'une importante ??tude isotopique des verres de r??f??rence USGS GSD-1G et MPI-DING. Treize laboratoires diff??rents ont particip?? au travers de techniques analytiques de haute pr??cision travaillant soit sur ??chantillon total (TIMS, MC-ICP-MS) soit par microanalyse ??in situ?? (LA-MC-ICP-MS, LA-ICP-MS). ?? 2010 The Authors. Geostandards and Geoanalytical Research ?? 2010 International Association of Geoanalysts.

Epigenetic regulation of APC in the molecular pathogenesis of gallbladder cancer.

PubMed

Tekcham, Dinesh Singh; Poojary, Satish S; Bhunia, Shushruta; Barbhuiya, Mustafa Ahmed; Gupta, Sanjeev; Shrivastav, Braj Raj; Tiwari, Pramod Kumar

2016-05-01

Loss of function of adenomatous polyposis coli (APC) has been reported in cancer. The two promoters of APC, 1A and 1B also have roles in cancer. But, the epigenetic role of APC promoters is not yet clear in gallbladder cancer (GBC) and gallstone diseases (GSD). We undertook this study to determine the epigenetic role of APC in GBC and GSD. Methylation-specific (MS)-PCR was used to analyze the methylation of APC gene. The expression of APC gene was studied by semi-quantitative PCR, real-time PCR and immunohistochemistry (IHC) in GBC, GSD and adjacent normal tissues. Of the two promoters, APC 1A promoter was found methylated in 96 per cent GBC ( P=0.0155) and 80 per cent GSD (P=0.015). Exon 1 was downregulated in grade II (P=0.002) and grade III (P=0.0001) of GBC, while exon 2 was normally expressed. Scoring analysis of IHC revealed 0 or negativity in 34.48 per cent (P=0.057) and 1+ in 24.14 per cent (P=0.005) GBC cases suggesting loss of APC expression. The present findings indicate epigenetic silencing of APC in advanced GBC. The methylation pattern, followed by expression analysis of APC may be suggested for diagnostic, prognostic and therapeutic purposes in GBC in future.

Comparison of Learning Styles of Pharmacy Students and Faculty Members

PubMed Central

Crawford, Stephanie Y.; Alhreish, Suhail K.

2012-01-01

Objectives. To compare dominant learning styles of pharmacy students and faculty members and between faculty members in different tracks. Methods. Gregorc Style Delineator (GSD) and Zubin’s Pharmacists’ Inventory of Learning Styles (PILS) were administered to students and faculty members at an urban, Midwestern college of pharmacy. Results. Based on responses from 299 students (classes of 2008, 2009, and 2010) and 59 faculty members, GSD styles were concrete sequential (48%), abstract sequential (18%), abstract random (13%), concrete random (13%), and multimodal (8%). With PILS, dominant styles were assimilator (47%) and converger (30%). There were no significant differences between faculty members and student learning styles nor across pharmacy student class years (p>0.05). Learning styles differed between men and women across both instruments (p<0.01), and between faculty members in tenure and clinical tracks for the GSD styles (p=0.01). Conclusion. Learning styles differed among respondents based on gender and faculty track. PMID:23275657

Cigarettes vs. e-cigarettes: Passive exposure at home measured by means of airborne marker and biomarkers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ballbè, Montse; Catalan Network of Smoke-free Hospitals, L'Hospitalet de Llobregat, Barcelona; Cancer Prevention and Control Group, Institut d'Investigació Biomèdica de Bellvitge – IDIBELL, L’Hospitalet de Llobregat, Barcelona

Background: There is scarce evidence about passive exposure to the vapour released or exhaled from electronic cigarettes (e-cigarettes) under real conditions. The aim of this study is to characterise passive exposure to nicotine from e-cigarettes' vapour and conventional cigarettes' smoke at home among non-smokers under real-use conditions. Methods: We conducted an observational study with 54 non-smoker volunteers from different homes: 25 living at home with conventional smokers, 5 living with nicotine e-cigarette users, and 24 from control homes (not using conventional cigarettes neither e-cigarettes). We measured airborne nicotine at home and biomarkers (cotinine in saliva and urine). We calculated geometricmore » mean (GM) and geometric standard deviations (GSD). We also performed ANOVA and Student's t tests for the log-transformed data. We used Bonferroni-corrected t-tests to control the family error rate for multiple comparisons at 5%. Results: The GMs of airborne nicotine were 0.74 μg/m{sup 3} (GSD=4.05) in the smokers’ homes, 0.13 μg/m{sup 3} (GSD=2.4) in the e-cigarettes users’ homes, and 0.02 μg/m{sup 3} (GSD=3.51) in the control homes. The GMs of salivary cotinine were 0.38 ng/ml (GSD=2.34) in the smokers’ homes, 0.19 ng/ml (GSD=2.17) in the e-cigarettes users’ homes, and 0.07 ng/ml (GSD=1.79) in the control homes. Salivary cotinine concentrations of the non-smokers exposed to e-cigarette's vapour at home (all exposed ≥2 h/day) were statistically significant different that those found in non-smokers exposed to second-hand smoke ≥2 h/day and in non-smokers from control homes. Conclusions: The airborne markers were statistically higher in conventional cigarette homes than in e-cigarettes homes (5.7 times higher). However, concentrations of both biomarkers among non-smokers exposed to conventional cigarettes and e-cigarettes’ vapour were statistically similar (only 2 and 1.4 times higher, respectively). The levels of airborne nicotine and cotinine concentrations in the homes with e-cigarette users were higher than control homes (differences statistically significant). Our results show that non-smokers passively exposed to e-cigarettes absorb nicotine. - Highlights: • This is the first study of e-cigarette exposure at home under real-use conditions. • Airborne nicotine in homes with smokers were 5.7 times higher than in e-cig homes. • Cotinine of non-smokers exposed to e-cig and conventional cigarettes was similar. • Airborne nicotine in homes with e-cig users was higher than control homes. • Cotinine of non-smokers exposed to e-cig users was higher than in those no exposed.« less

The effect of guided self-determination on self-management in persons with type 1 diabetes mellitus and HbA1c ≥64 mmol/mol: a group-based randomised controlled trial.

PubMed

Mohn, Jannike; Graue, Marit; Assmus, Jõrg; Zoffmann, Vibeke; Thordarson, Hrafnkell; Peyrot, Mark; Rokne, Berit

2017-07-03

To determine whether the impact of guided self-determination (GSD) applied in group training (GSD-GT) in people with chronically elevated HbA 1c and type 1 diabetes mellitus (DM) was superior to 'care as usual' in improving HbA 1c and psychological functioning. An outpatient clinic at a university hospital in Western Norway. A total of 178 adults (all Caucasian) aged 18-55 (mean age 36.7±10.7, 62% women) with type 1 DM for at least 1 year and HbA 1c ≥64 mmol/mol (8.0%) were randomly assigned to participate in either GSD-GT or a control group (CG). Exclusion criteria were severe comorbidity, major psychiatric disorder, cognitive deficiency/language barriers and pregnancy. Intervention group met seven times for 2 hours over 14 weeks to promote patient autonomy and intrinsic motivation using reflection sheets and advanced professional communication in accordance with the GSD methodology. The primary outcome was HbA 1c and secondary outcomes (all outcomes 9 months post intervention) were self-monitored blood glucose frequency, self-reported diabetes competence, autonomy support by healthcare providers (Health Care Climate Questionnaire), autonomous versus controlled diabetes motivation (Treatment Self-Regulation Questionnaire), diabetes distress (Problem Areas In Diabetes Scale (PAID) and Diabetes Distress Scale (DDS)), self-esteem (Rosenberg Self-Esteem Scale) and psychological well-being (World Health Organization five-item Well-Being Index scale). Among participants allocated to the GSD-GT (=90) 48 completed the study, whereas 83 completed in the CG (n=88). With 95% CIs GSD-GT did not have effect on HbA 1c (B -0.18, CI (-0.48, 0.12), p=0.234). GSD-GT improved autonomy-motivated behaviour (B 0.51, CI (0.25, 0.77), p<0.001), diabetes distress (PAID, B -6.96, CI (-11.40, -2.52), p=0.002), total DDS (B -5.15, CI (-9.34, -0.96), p=0.016), DDS emotional burden (B -7.19, CI (-13.20, -1.19), p=0.019) and self-esteem (B 1.43, CI (0.34, 2.52), p=0.011). Results from this behavioural intervention must be interpreted cautiously because of recruitment and attrition problems. Medical outcomes did not improve. Psychological outcomes improved, especially reduced diabetes distress. Clinical Trials.gov NCT 01317459. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

The effect of guided self-determination on self-management in persons with type 1 diabetes mellitus and HbA1c ≥64 mmol/mol: a group-based randomised controlled trial

PubMed Central

Mohn, Jannike; Graue, Marit; Assmus, Jõrg; Zoffmann, Vibeke; Thordarson, Hrafnkell; Peyrot, Mark; Rokne, Berit

2017-01-01

Objectives To determine whether the impact of guided self-determination (GSD) applied in group training (GSD-GT) in people with chronically elevated HbA1c and type 1 diabetes mellitus (DM) was superior to ‘care as usual’ in improving HbA1c and psychological functioning. Setting An outpatient clinic at a university hospital in Western Norway. Participants A total of 178 adults (all Caucasian) aged 18–55 (mean age 36.7±10.7, 62% women) with type 1 DM for at least 1 year and HbA1c ≥64 mmol/mol (8.0%) were randomly assigned to participate in either GSD-GT or a control group (CG). Exclusion criteria were severe comorbidity, major psychiatric disorder, cognitive deficiency/language barriers and pregnancy. Intervention Intervention group met seven times for 2 hours over 14 weeks to promote patient autonomy and intrinsic motivation using reflection sheets and advanced professional communication in accordance with the GSD methodology. Primary and secondary outcome measures The primary outcome was HbA1c and secondary outcomes (all outcomes 9 months post intervention) were self-monitored blood glucose frequency, self-reported diabetes competence, autonomy support by healthcare providers (Health Care Climate Questionnaire), autonomous versus controlled diabetes motivation (Treatment Self-Regulation Questionnaire), diabetes distress (Problem Areas In Diabetes Scale (PAID) and Diabetes Distress Scale (DDS)), self-esteem (Rosenberg Self-Esteem Scale) and psychological well-being (World Health Organization five-item Well-Being Index scale). Results Among participants allocated to the GSD-GT (=90) 48 completed the study, whereas 83 completed in the CG (n=88). With 95% CIs GSD-GT did not have effect on HbA1c (B −0.18, CI (−0.48, 0.12), p=0.234). GSD-GT improved autonomy-motivated behaviour (B 0.51, CI (0.25, 0.77), p<0.001), diabetes distress (PAID, B −6.96, CI (−11.40, −2.52), p=0.002), total DDS (B −5.15, CI (−9.34, −0.96), p=0.016), DDS emotional burden (B −7.19, CI (−13.20, −1.19), p=0.019) and self-esteem (B 1.43, CI (0.34, 2.52), p=0.011). Conclusions Results from this behavioural intervention must be interpreted cautiously because of recruitment and attrition problems. Medical outcomes did not improve. Psychological outcomes improved, especially reduced diabetes distress. Trial registration number Clinical Trials.gov NCT 01317459. PMID:28674125

Flow regulation in the Swiss Alps: a river network modelling approach to investigate the impacts on bed load and grain size distribution

NASA Astrophysics Data System (ADS)

Costa, A.; Molnar, P.; Schmitt, R. J. P.

2017-12-01

The grain size distribution (GSD) of river bed sediment results from the long term balance between transport capacity and sediment supply. Changes in climate and human activities may alter the spatial distribution of transport capacity and sediment supply along channels and hence impact local bedload transport and GSD. The effects of changed flow are not easily inferable due the non-linear, threshold-based nature of the relation between discharge and sediment mobilization, and the network-scale control on local sediment supply. We present a network-scale model for fractional sediment transport to quantify the impact of hydropower (HP) operations on river network GSD. We represent the river network as a series of connected links for which we extract the geometric characteristics from satellite images and a digital elevation model. We assign surface roughness based on the channel bed GSD. Bed shear stress is estimated at link-scale under the assumptions of rectangular prismatic cross sections and normal flow. The mass balance between sediment supply and transport capacity, computed with the Wilcock and Crowe model, determines transport rates of multiple grain size classes and the resulting GSD. We apply the model to the upper Rhone basin, a large Alpine basin in Switzerland. Since 1960s, changed flow conditions due to HP operations and sediment storage behind dams have potentially altered the sediment transport of the basin. However, little is known on the magnitude and spatial distribution of these changes. We force the model with time series of daily discharge derived with a spatially distributed hydrological model for pre and post HP scenarios. We initialize GSD under the assumption that coarse grains (d90) are mobilized only during mean annual maximum flows, and on the basis of ratios between d90 and characteristic diameters estimated from field measurements. Results show that effects of flow regulation vary significantly in space and in time and are grain size dependent. HP operations led to an overall reduction of sediment transport at network scale, especially in summer and for coarser grains, leading to a general coarsening of the river bed sediments at the upstream reaches. The model allows investigating the impact of modified HP operations and climate change projections on sediment dynamics at the network scale.

Current Chinese Leadership: Background, Associations, and Issues for Selected Key Military Leaders

DTIC Science & Technology

1985-09-01

58 years old). Key leaders classified as conservative, such as Ye Jianying and Xu Xiangqian, lost their influential positions in September 1985...Cmte, CCP-CC (since Oct 83); VC, Central Greening Cmte (since Feb 82) Name;Jiang Xianchen Position; Deputy Chief, PLA GSD (since Mar 85) Name; Li ...Guang Position; Dir, Weapons & Equipment Dept, PLA GSD (since Nov 82) Name; Li Jing Position; Dep Cdr, PLA Navy (since Jul 83) Name; Li Jiulong

Glucose-6-phosphate transporter gene therapy corrects metabolic and myeloid abnormalities in glycogen storage disease type Ib mice

PubMed Central

Yiu, Wai Han; Pan, Chi-Jiunn; Allamarvdasht, Mohammad; Kim, So Youn; Chou, Janice Y.

2008-01-01

Glycogen storage disease type Ib (GSD-Ib) is caused by a deficiency in the glucose-6-phosphate transporter (G6PT), an endoplasmic reticulum-associated transmembrane protein that is ubiquitously expressed. GSD-Ib patients suffer from disturbed glucose homeostasis and myeloid dysfunctions. To evaluate the feasibility of gene replacement therapy for GSD-Ib, we have infused adenoviral (Ad) vector containing human G6PT (Ad-hG6PT) into G6PT-deficient (G6PT-/-) mice that manifest symptoms characteristics of the human disorder. Ad-hG6PT-infusion restores significant levels of G6PT mRNA expression in the liver, bone marrow, and spleen and corrects metabolic as well as myeloid abnormalities in G6PT-/- mice. The G6PT-/- mice receiving gene therapy exhibit improved growth; normalized serum profiles for glucose, cholesterol, triglyceride, uric acid, and lactic acid; and reduced hepatic glycogen deposition. The therapy also corrects neutropenia and lowers the elevated serum levels of granulocyte colony stimulating factor. The development of bone and spleen in the infused G6PT-/- mice is improved and accompanied by increased cellularity and normalized myeloid progenitor cell frequencies in both tissues. This effective use of gene therapy to correct metabolic imbalances and myeloid dysfunctions in GSD-Ib mice holds promise for the future of gene therapy in humans. PMID:17006547

Characterizing pulmonary blood flow distribution measured using arterial spin labeling.

PubMed

Henderson, A Cortney; Prisk, G Kim; Levin, David L; Hopkins, Susan R; Buxton, Richard B

2009-12-01

The arterial spin labeling (ASL) method provides images in which, ideally, the signal intensity of each image voxel is proportional to the local perfusion. For studies of pulmonary perfusion, the relative dispersion (RD, standard deviation/mean) of the ASL signal across a lung section is used as a reliable measure of flow heterogeneity. However, the RD of the ASL signals within the lung may systematically differ from the true RD of perfusion because the ASL image also includes signals from larger vessels, which can reflect the blood volume rather than blood flow if the vessels are filled with tagged blood during the imaging time. Theoretical studies suggest that the pulmonary vasculature exhibits a lognormal distribution for blood flow and thus an appropriate measure of heterogeneity is the geometric standard deviation (GSD). To test whether the ASL signal exhibits a lognormal distribution for pulmonary blood flow, determine whether larger vessels play an important role in the distribution, and extract physiologically relevant measures of heterogeneity from the ASL signal, we quantified the ASL signal before and after an intervention (head-down tilt) in six subjects. The distribution of ASL signal was better characterized by a lognormal distribution than a normal distribution, reducing the mean squared error by 72% (p < 0.005). Head-down tilt significantly reduced the lognormal scale parameter (p = 0.01) but not the shape parameter or GSD. The RD increased post-tilt and remained significantly elevated (by 17%, p < 0.05). Test case results and mathematical simulations suggest that RD is more sensitive than the GSD to ASL signal from tagged blood in larger vessels, a probable explanation of the change in RD without a statistically significant change in GSD. This suggests that the GSD is a useful measure of pulmonary blood flow heterogeneity with the advantage of being less affected by the ASL signal from tagged blood in larger vessels.

Written reflection in an eHealth intervention for adults with type 2 diabetes mellitus: a qualitative study.

PubMed

Lie, Silje S; Karlsen, Bjørg; Niemiec, Christopher P; Graue, Marit; Oftedal, Bjørg

2018-01-01

Individuals with type 2 diabetes mellitus (T2DM) are responsible for the daily decisions and actions necessary to manage their disease, which makes self-management the cornerstone of diabetes care. Many patients do not reach recommended treatment goals, and thus it is important to develop and evaluate innovative interventions that facilitate optimal motivation for adequate self-management of T2DM. The aim of the current study was to explore how adults with T2DM experience using reflection sheets to stimulate written reflection in the context of the Guided Self-Determination (GSD) eHealth intervention and how written reflection might affect their motivation for self-management of T2DM. We used a qualitative design in which data were collected through individual interviews. The sample consisted of 10 patients who completed the GSD eHealth intervention, and data were analyzed using qualitative content analysis. The qualitative content analysis yielded 2 main themes. We labeled the first theme as "Written reflection affects awareness and commitment in diabetes self-management", which reflects 2 subthemes, namely, "Writing creates space and time for autonomous reflection" and "Writing influences individuals' focus in diabetes self-management". We labeled the second theme as "Written reflection is perceived as inapplicable in diabetes self-management", which reflects 2 subthemes, namely, "Responding in writing is difficult" and "The timing of the writing is inappropriate". Our findings indicate that written reflection in the context of the GSD eHealth intervention may be conducive to motivation for diabetes self-management for some patients. However, it seems that in-person consultation with the diabetes nurse may be necessary to achieve the full potential benefit of the GSD as an eHealth intervention. We advocate further development and examination of the GSD as a "blended" approach, especially for those who consider written reflection to be difficult or unfamiliar.

Long-term efficacy following readministration of an adeno-associated virus vector in dogs with glycogen storage disease type Ia.

PubMed

Demaster, Amanda; Luo, Xiaoyan; Curtis, Sarah; Williams, Kyha D; Landau, Dustin J; Drake, Elizabeth J; Kozink, Daniel M; Bird, Andrew; Crane, Bayley; Sun, Francis; Pinto, Carlos R; Brown, Talmage T; Kemper, Alex R; Koeberl, Dwight D

2012-04-01

Glycogen storage disease type Ia (GSD-Ia) is the inherited deficiency of glucose-6-phosphatase (G6Pase), primarily found in liver and kidney, which causes life-threatening hypoglycemia. Dogs with GSD-Ia were treated with double-stranded adeno-associated virus (AAV) vectors encoding human G6Pase. Administration of an AAV9 pseudotyped (AAV2/9) vector to seven consecutive GSD-Ia neonates prevented hypoglycemia during fasting for up to 8 hr; however, efficacy eventually waned between 2 and 30 months of age, and readministration of a new pseudotype was eventually required to maintain control of hypoglycemia. Three of these dogs succumbed to acute hypoglycemia between 7 and 9 weeks of age; however, this demise could have been prevented by earlier readministration an AAV vector, as demonstrated by successful prevention of mortality of three dogs treated earlier in life. Over the course of this study, six out of nine dogs survived after readministration of an AAV vector. Of these, each dog required readministration on average every 9 months. However, two were not retreated until >34 months of age, while one with preexisting antibodies was re-treated three times in 10 months. Glycogen content was normalized in the liver following vector administration, and G6Pase activity was increased in the liver of vector-treated dogs in comparison with GSD-Ia dogs that received only with dietary treatment. G6Pase activity reached approximately 40% of normal in two female dogs following AAV2/9 vector administration. Elevated aspartate transaminase in absence of inflammation indicated that hepatocellular turnover in the liver might drive the loss of vector genomes. Survival was prolonged for up to 60 months in dogs treated by readministration, and all dogs treated by readministration continue to thrive despite the demonstrated risk for recurrent hypoglycemia and mortality from waning efficacy of the AAV2/9 vector. These preclinical data support the further translation of AAV vector-mediated gene therapy in GSD-Ia.

Detecting Uniform Areas for Vicarious Calibration using Landsat TM Imagery: A Study using the Arabian and Saharan Deserts

NASA Technical Reports Server (NTRS)

Hilbert, Kent; Pagnutti, Mary; Ryan, Robert; Zanoni, Vicki

2002-01-01

This paper discusses a method for detecting spatially uniform sites need for radiometric characterization of remote sensing satellites. Such information is critical for scientific research applications of imagery having moderate to high resolutions (<30-m ground sampling distance (GSD)). Previously published literature indicated that areas with the African Saharan and Arabian deserts contained extremely uniform sites with respect to spatial characteristics. We developed an algorithm for detecting site uniformity and applied it to orthorectified Landsat Thematic Mapper (TM) imagery over eight uniform regions of interest. The algorithm's results were assessed using both medium-resolution (30-m GSD) Landsat 7 ETM+ and fine-resolution (<5-m GSD) IKONOS multispectral data collected over sites in Libya and Mali. Fine-resolution imagery over a Libyan site exhibited less than 1 percent nonuniformity. The research shows that Landsat TM products appear highly useful for detecting potential calibration sites for system characterization. In particular, the approach detected spatially uniform regions that frequently occur at multiple scales of observation.

The structure optimization of gas-phase surface discharge and its application for dye degradation

NASA Astrophysics Data System (ADS)

Ying, CAO; Jie, LI; Nan, JIANG; Yan, WU; Kefeng, SHANG; Na, LU

2018-05-01

A gas-phase surface discharge (GSD) was employed to optimize the discharge reactor structure and investigate the dye degradation. A dye mixture of methylene blue, acid orange and methyl orange was used as a model pollutant. The results indicated that the reactor structure of the GSD system with the ratio of tube inner surface area and volume of 2.48, screw pitch between a high-voltage electrode of 9.7 mm, high-voltage electrode wire diameter of 0.8 mm, dielectric tube thickness of 2.0 mm and tube inner diameter of 16.13 mm presented a better ozone (O3) generation efficiency. Furthermore, a larger screw pitch and smaller wire diameter enhanced the O3 generation. After the dye mixture degradation by the optimized GSD system, 73.21% and 50.74% of the chemical oxygen demand (COD) and total organic carbon removal rate were achieved within 20 min, respectively, and the biochemical oxygen demand (BOD) and biodegradability (BOD/COD) improved.

Parallel implementation of geometrical shock dynamics for two dimensional converging shock waves

NASA Astrophysics Data System (ADS)

Qiu, Shi; Liu, Kuang; Eliasson, Veronica

2016-10-01

Geometrical shock dynamics (GSD) theory is an appealing method to predict the shock motion in the sense that it is more computationally efficient than solving the traditional Euler equations, especially for converging shock waves. However, to solve and optimize large scale configurations, the main bottleneck is the computational cost. Among the existing numerical GSD schemes, there is only one that has been implemented on parallel computers, with the purpose to analyze detonation waves. To extend the computational advantage of the GSD theory to more general applications such as converging shock waves, a numerical implementation using a spatial decomposition method has been coupled with a front tracking approach on parallel computers. In addition, an efficient tridiagonal system solver for massively parallel computers has been applied to resolve the most expensive function in this implementation, resulting in an efficiency of 0.93 while using 32 HPCC cores. Moreover, symmetric boundary conditions have been developed to further reduce the computational cost, achieving a speedup of 19.26 for a 12-sided polygonal converging shock.

Origin and transition of sex determination mechanisms in a gynogenetic hexaploid fish.

PubMed

Li, Xi-Yin; Liu, Xiao-Li; Zhu, Yao-Jun; Zhang, Jun; Ding, Miao; Wang, Ming-Tao; Wang, Zhong-Wei; Li, Zhi; Zhang, Xiao-Juan; Zhou, Li; Gui, Jian-Fang

2018-02-02

Most vertebrates reproduce sexually, and plastic sex determination mechanisms including genotypic sex determination (GSD) and environmental sex determination (ESD) have been extensively revealed. However, why sex determination mechanisms evolve diversely and how they correlate with diverse reproduction strategies remain largely unclear. Here, we utilize the superiority of a hexaploid gibel carp (Carassius gibelio) that is able to reproduce by unisexual gynogenesis and contains a rare but diverse proportion of males to investigate these puzzles. A total of 2248 hexaploid specimens were collected from 34 geographic wild populations throughout mainland China, in which 24 populations were revealed to contain 186 males with various incidences ranging from 1.2 to 26.5%. Subsequently, the proportion of temperature-dependent sex determination (TSD) was revealed to be positively correlated to average annual temperature in wild populations, and male incidence in lab gynogenetic progenies was demonstrated to increase with the increasing of larval rearing temperature. Meanwhile, extra microchromosomes were confirmed to play genotypic male determination role as previously reported. Thereby, GSD and TSD were found to coexist in gibel carp, and the proportions of GSD were observed to be much higher than that of TSD in sympatric wild populations. Our findings uncover a potential new mechanism in the evolution of sex determination system in polyploid vertebrates with unisexual gynogenesis ability, and also reveal a possible association of sex determination mechanism transition between TSD and GSD and reproduction mode transition between unisexual gynogenesis and bisexual reproduction.

Using Remote Sensing to Determine the Spatial Scales of Estuaries

NASA Astrophysics Data System (ADS)

Davis, C. O.; Tufillaro, N.; Nahorniak, J.

2016-02-01

One challenge facing Earth system science is to understand and quantify the complexity of rivers, estuaries, and coastal zone regions. Earlier studies using data from airborne hyperspectral imagers (Bissett et al., 2004, Davis et al., 2007) demonstrated from a very limited data set that the spatial scales of the coastal ocean could be resolved with spatial sampling of 100 m Ground Sample Distance (GSD) or better. To develop a much larger data set (Aurin et al., 2013) used MODIS 250 m data for a wide range of coastal regions. Their conclusion was that farther offshore 500 m GSD was adequate to resolve large river plume features while nearshore regions (a few kilometers from the coast) needed higher spatial resolution data not available from MODIS. Building on our airborne experience, the Hyperspectral Imager for the Coastal Ocean (HICO, Lucke et al., 2011) was designed to provide hyperspectral data for the coastal ocean at 100 m GSD. HICO operated on the International Space Station for 5 years and collected over 10,000 scenes of the coastal ocean and other regions around the world. Here we analyze HICO data from an example set of major river delta regions to assess the spatial scales of variability in those systems. In one system, the San Francisco Bay and Delta, we also analyze Landsat 8 OLI data at 30 m and 15 m to validate the 100 m GSD sampling scale for the Bay and assess spatial sampling needed as you move up river.

Long-Term Efficacy Following Readministration of an Adeno-Associated Virus Vector in Dogs with Glycogen Storage Disease Type Ia

PubMed Central

Demaster, Amanda; Luo, Xiaoyan; Curtis, Sarah; Williams, Kyha D.; Landau, Dustin J.; Drake, Elizabeth J.; Kozink, Daniel M.; Bird, Andrew; Crane, Bayley; Sun, Francis; Pinto, Carlos R.; Brown, Talmage T.; Kemper, Alex R.

2012-01-01

Abstract Glycogen storage disease type Ia (GSD-Ia) is the inherited deficiency of glucose-6-phosphatase (G6Pase), primarily found in liver and kidney, which causes life-threatening hypoglycemia. Dogs with GSD-Ia were treated with double-stranded adeno-associated virus (AAV) vectors encoding human G6Pase. Administration of an AAV9 pseudotyped (AAV2/9) vector to seven consecutive GSD-Ia neonates prevented hypoglycemia during fasting for up to 8 hr; however, efficacy eventually waned between 2 and 30 months of age, and readministration of a new pseudotype was eventually required to maintain control of hypoglycemia. Three of these dogs succumbed to acute hypoglycemia between 7 and 9 weeks of age; however, this demise could have been prevented by earlier readministration an AAV vector, as demonstrated by successful prevention of mortality of three dogs treated earlier in life. Over the course of this study, six out of nine dogs survived after readministration of an AAV vector. Of these, each dog required readministration on average every 9 months. However, two were not retreated until >34 months of age, while one with preexisting antibodies was re-treated three times in 10 months. Glycogen content was normalized in the liver following vector administration, and G6Pase activity was increased in the liver of vector-treated dogs in comparison with GSD-Ia dogs that received only with dietary treatment. G6Pase activity reached approximately 40% of normal in two female dogs following AAV2/9 vector administration. Elevated aspartate transaminase in absence of inflammation indicated that hepatocellular turnover in the liver might drive the loss of vector genomes. Survival was prolonged for up to 60 months in dogs treated by readministration, and all dogs treated by readministration continue to thrive despite the demonstrated risk for recurrent hypoglycemia and mortality from waning efficacy of the AAV2/9 vector. These preclinical data support the further translation of AAV vector–mediated gene therapy in GSD-Ia. PMID:22185325

Hepatic lentiviral gene transfer prevents the long-term onset of hepatic tumours of glycogen storage disease type 1a in mice.

PubMed

Clar, Julie; Mutel, Elodie; Gri, Blandine; Creneguy, Alison; Stefanutti, Anne; Gaillard, Sophie; Ferry, Nicolas; Beuf, Olivier; Mithieux, Gilles; Nguyen, Tuan Huy; Rajas, Fabienne

2015-04-15

Glycogen storage disease type 1a (GSD1a) is a rare disease due to the deficiency in the glucose-6-phosphatase (G6Pase) catalytic subunit (encoded by G6pc), which is essential for endogenous glucose production. Despite strict diet control to maintain blood glucose, patients with GSD1a develop hepatomegaly, steatosis and then hepatocellular adenomas (HCA), which can undergo malignant transformation. Recently, gene therapy has attracted attention as a potential treatment for GSD1a. In order to maintain long-term transgene expression, we developed an HIV-based vector, which allowed us to specifically express the human G6PC cDNA in the liver. We analysed the efficiency of this lentiviral vector in the prevention of the development of the hepatic disease in an original GSD1a mouse model, which exhibits G6Pase deficiency exclusively in the liver (L-G6pc(-/-) mice). Recombinant lentivirus were injected in B6.G6pc(ex3lox/ex3lox). SA(creERT2/w) neonates and G6pc deletion was induced by tamoxifen treatment at weaning. Magnetic resonance imaging was then performed to follow up the development of hepatic tumours. Lentiviral gene therapy restored glucose-6 phosphatase activity sufficient to correct fasting hypoglycaemia during 9 months. Moreover, lentivirus-treated L-G6pc(-/-) mice presented normal hepatic triglyceride levels, whereas untreated mice developed steatosis. Glycogen stores were also decreased although liver weight remained high. Interestingly, lentivirus-treated L-G6pc(-/-) mice were protected against the development of hepatic tumours after 9 months of gene therapy while most of untreated L-G6pc(-/-) mice developed millimetric HCA. Thus the treatment of newborns by recombinant lentivirus appears as an attractive approach to protect the liver from the development of steatosis and hepatic tumours associated to GSD1a pathology. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Transcriptomic responses to environmental temperature by turtles with temperature-dependent and genotypic sex determination assessed by RNAseq inform the genetic architecture of embryonic gonadal development.

PubMed

Radhakrishnan, Srihari; Literman, Robert; Neuwald, Jennifer; Severin, Andrew; Valenzuela, Nicole

2017-01-01

Vertebrate sexual fate is decided primarily by the individual's genotype (GSD), by the environmental temperature during development (TSD), or both. Turtles exhibit TSD and GSD, making them ideal to study the evolution of sex determination. Here we analyze temperature-specific gonadal transcriptomes (RNA-sequencing validated by qPCR) of painted turtles (Chrysemys picta TSD) before and during the thermosensitive period, and at equivalent stages in soft-shell turtles (Apalone spinifera-GSD), to test whether TSD's and GSD's transcriptional circuitry is identical but deployed differently between mechanisms. Our data show that most elements of the mammalian urogenital network are active during turtle gonadogenesis, but their transcription is generally more thermoresponsive in TSD than GSD, and concordant with their sex-specific function in mammals [e.g., upregulation of Amh, Ar, Esr1, Fog2, Gata4, Igf1r, Insr, and Lhx9 at male-producing temperature, and of β-catenin, Foxl2, Aromatase (Cyp19a1), Fst, Nf-kb, Crabp2 at female-producing temperature in Chrysemys]. Notably, antagonistic elements in gonadogenesis (e.g., β-catenin and Insr) were thermosensitive only in TSD early-embryos. Cirbp showed warm-temperature upregulation in both turtles disputing its purported key TSD role. Genes that may convert thermal inputs into sex-specific development (e.g., signaling and hormonal pathways, RNA-binding and heat-shock) were differentially regulated. Jak-Stat, Nf-κB, retinoic-acid, Wnt, and Mapk-signaling (not Akt and Ras-signaling) potentially mediate TSD thermosensitivity. Numerous species-specific ncRNAs (including Xist) were differentially-expressed, mostly upregulated at colder temperatures, as were unannotated loci that constitute novel TSD candidates. Cirbp showed warm-temperature upregulation in both turtles. Consistent transcription between turtles and alligator revealed putatively-critical reptilian TSD elements for male (Sf1, Amh, Amhr2) and female (Crabp2 and Hspb1) gonadogenesis. In conclusion, while preliminary, our data helps illuminate the regulation and evolution of vertebrate sex determination, and contribute genomic resources to guide further research into this fundamental biological process.

A Gravimetric Geoid Model for Vertical Datum in Canada

NASA Astrophysics Data System (ADS)

Veronneau, M.; Huang, J.

2004-05-01

The need to realize a new vertical datum for Canada dates back to 1976 when a study group at Geodetic Survey Division (GSD) investigated problems related to the existing vertical system (CGVD28) and recommended a redefinition of the vertical datum. The US National Geodetic Survey and GSD cooperated in the development of a new North American Vertical Datum (NAVD88). Although the USA adopted NAVD88 in 1993 as its datum, Canada declined to do so as a result of unexplained discrepancies of about 1.5 m from east to west coasts (likely due to systematic errors). The high cost of maintaining the vertical datum by the traditional spirit leveling technique coupled with budgetary constraints has forced GSD to modify its approach. A new attempt (project) to modernize the vertical datum is currently in process in Canada. The advance in space-based technologies (e.g. GPS, satellite radar altimetry, satellite gravimetry) and new developments in geoid modeling offer an alternative to spirit leveling. GSD is planning to implement, after stakeholder consultations, a geoid model as the new vertical datum for Canada, which will allow space-based technology users access to an accurate and uniform datum all across the Canadian landmass and surrounding oceans. CGVD28 is only accessible through a limited number of benchmarks, primarily located in southern Canada. The new vertical datum would be less sensitive to geodynamic activities (post-glacial rebound and earthquake), local uplift and subsidence, and deterioration of the benchmarks. The adoption of a geoid model as a vertical datum does not mean that GSD is neglecting the current benchmarks. New heights will be given to the benchmarks by a new adjustment of the leveling observations, which will be constrained to the geoid model at selected stations of the Active Control System (ACS) and Canadian Base Network (CBN). This adjustment will not correct vertical motion at benchmarks, which has occurred since the last leveling observations. The presentation provides an overview of the "Height Modernization" project, and discusses the accuracy of the existing geoid models in Canada.

Anesthetic gas exposure in veterinary clinics.

PubMed

Korczynski, R E

1999-06-01

Concerns were raised by several workers from veterinary clinics in Manitoba, Canada, regarding potential exposure to isoflurane and halothane during anesthetic administration. There has been no guideline established for isoflurane by the American Conference of Governmental Industrial Hygienist (ACGIH) or a Permissible Exposure Limit by the Occupational Safety and Health Administration (OSHA) or a recommended exposure limit (REL) by the National Institute for Occupational Safety and Health (NIOSH). The ACGIH TLV-TWA for halothane is 50 ppm and NIOSH has established 2 ppm as a recommended level based on an one-hour sampling. OSHA has established no guideline for halothane. The Miran IB Portable Ambient Air Analyzer was used to conduct real-time sampling and to identify leaks during administration. All veterinary clinics inspected had installed the passive waste gas scavenging system. Ten clinics were each monitored during anesthetic gas delivery for one surgical procedure performed. Induction was 4 to 5 percent and maintenance 1.5 to 2.5 percent. Nine clinics were small animal practices and the tenth was an equine clinic. Veterinarians' personal exposures were higher than the assistants'. Veterinarians' personal exposures for isoflurane ranged from 1.3 to 13 ppm (AM = 5.3; SD +/- 2.7; GM = 4.6; GSD +/- 1.6) and for their assistants, personal exposures ranged from 1.2 to 9 ppm (AM = 4.7; SD +/- 2.5; GM = 3.9; GSD +/- 1.6). Veterinarians' personal exposures for halothane ranged from 0.7 to 12 ppm (AM = 4.2; SD +/- 3.6; GM = 2.9; GSD +/- 1.4) and for their assistants, personal exposures ranged from 0.4 to 3.2 ppm (AM = 1.8; SD +/- 1.0; GM = 1.5; GSD +/- 1.7). One clinic had significant leaks in the anesthetic gas delivery lines. Personal halothane exposure for the veterinarian at this clinic was 7.2 to 65 ppm (AM = 18.0; SD +/- 11.5; GM = 15.9; GSD +/- 1.8). Based on this study, worker exposures were acceptable. Peak exposures were recorded when the cuffed endotracheal tube was removed from the animal. Equipment leaks were minimal when the system was maintained at its optimum operating condition.

Effects of two combined oral contraceptives containing ethinyl estradiol 30 microg combined with either gestodene or drospirenone on hemostatic parameters, lipid profiles and blood pressure.

PubMed

Yildizhan, Recep; Yildizhan, Begum; Adali, Ertan; Yoruk, Pinar; Birol, Fatih; Suer, Necdet

2009-08-01

The aim of this study is to compare the effect of ethinyl estradiol 0.03 mg/gestodene 0.075 mg (EE/GSD) with ethinylestradiol 0.03 mg/drospirenone 3 mg (EE/DRSP) administered according to conventional 21/7 regimen on body mass index (BMI), blood pressure (BP), lipid metabolism and hemostatic parameters. In this study, 160 healthy women were randomized to EE/GSD mg or EE/DRSP for 12 months. Mean differences in BMI, high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C), total cholesterol (TC) levels and BP compared to baseline were assessed. One hundred and forty-five (89%) of the women completed all 12 treatment cycles. The subjects randomly assigned into two treatment groups. Group EE/GSD (n = 71) and group EE/DRSP (n = 72). In group B, BMI values were significantly lower than baseline at the sixth cycle. DRSP/EE had more favorable effects on BP than GSD/EE with the mean systolic and diastolic BPs remaining lower in the DRSP/EE group. The difference between the two preparations was not statistically significant at the end of the study. TC levels remained similar in both groups throughout the study period. In both groups LDL-C levels decreased, triglyceride and HDL-C levels significantly increased from baseline levels. These changes result in increasing HDL-C/LDL-C ratio, demonstrating anti-atherogenic effect. Menstrual cycle patterns and the incidence of adverse events were similar between groups. The duration of withdrawal bleeding decreased during the study for both groups and was similar. The EE/DRSP regimen provides good cycle control with reliable contraceptive efficacy and low incidence of adverse events. Compared with the EE/GSD preparation, the EE/DRSP preparation demonstrated a more favorable effect on BMI and BP with the mean BMI and mean BP remaining lower than baseline mean. The new formulation may be especially beneficial for women susceptible to body weight gain and rise in BP.

3'-UTR SNP rs2229611 in G6PC1 affects mRNA stability, expression and Glycogen Storage Disease type-Ia risk.

PubMed

Karthi, Sellamuthu; Rajeshwari, Mohan; Francis, Amirtharaj; Saravanan, Matheshwaran; Varalakshmi, Perumal; Houlden, Henry; Thangaraj, Kumarasamy; Ashokkumar, Balasubramaniem

2017-08-01

The frequency of rs2229611, previously reported in Chinese, Caucasians, Japanese and Hispanics, was investigated for the first time in Indian ethnicity. We analyzed its role in the progression of Glycogen Storage Disease type-Ia (GSD-Ia) and breast cancer. Genotype data on rs2229611 revealed that the risk of GSD-Ia was higher (P=0.0195) with CC compared to TT/TC genotypes, whereas no such correlation was observed with breast cancer cases. We observed a strong linkage disequilibrium (LD) among rs2229611 and other disease causing G6PC1 variants (|D'|=1, r 2 =1). Functional validation performed in HepG2 cells using luciferase constructs showed significant (P<0.05) decrease in expression than wild-type 3'-UTR due to curtailed mRNA stability. Furthermore, AU-rich elements (AREs) mediated regulation of G6PC1 expression characterized using 3'-UTR deletion constructs showed a prominent decrease in mRNA stability. We then examined whether miRNAs are involved in controlling G6PC1 expression using pmirGLO-UTR constructs, with evidence of more distinct inhibition in the reporter function with rs2229611. These data suggests that rs2229611 is a crucial regulatory SNP which in homozygous state leads to a more aggressive disease phenotype in GSD-Ia patients. The implication of this result is significant in predicting disease onset, progression and response to disease modifying treatments in patients with GSD-Ia. Copyright © 2017 Elsevier B.V. All rights reserved.

Determinants of endotoxin levels in carpets in New Zealand homes.

PubMed

Wickens, K; Douwes, J; Siebers, R; Fitzharris, P; Wouters, I; Doekes, G; Mason, K; Hearfield, M; Cunningham, M; Crane, J

2003-06-01

Endotoxin in house dust has been shown to be associated with asthma severity. Little is known about the influence of housing characteristics on endotoxin distribution. Using standardized methods, dust was sampled from a 1m(2) site and the whole accessible carpet area in selected Wellington, New Zealand homes (n = 77). Endotoxin was measured using a Limulus Amoebocyte Lysate assay. Relative humidity and temperature were recorded using sensors placed in carpet bases. Questionnaires were used to collect information on housing characteristics. All analyses were performed for endotoxin units (EU)/mg and EU/m2 for each site. Geometric mean endotoxin levels were 22.7 EU/mg [geometric standard deviation (GSD) = 2.4] or 30,544 EU/m2 (GSD = 3.2) from the 1m(2) site, and 28.4 EU/mg (GSD = 3.4) or 5653 EU/m2 (GSD = 6.4) from the whole room. After controlling for confounding, endotoxin was positively associated with dogs inside [geometric mean ratio (GMR): 0.9-2.0], total household occupants (GMR: 1.7-2.0, for 1 m2 sample only), vacuum cleaners <1-year old (GMR: 2.3-2.7), reusing vacuum dust collection bags (GMR: 1.4-3.1), steamcleaning or shampooing the carpet (GMR: 1.4-2.2) and high relative humidity (GMR: 1.4-1.6). Lower endotoxin was associated with floor insulation (GMR: 0.4-0.8), and north-facing living rooms (GMR: 0.4-0.8). This study has identified home characteristics that could be modified to reduce endotoxin exposure.

Prenatal diagnosis of glycogen storage disease type 1a by single stranded conformation polymorphism (SSCP).

PubMed

Parvari, R; Hershkovitz, E; Carmi, R; Moses, S

1996-09-01

Glycogen storage disease type 1a (GSD 1a), a severe metabolic disorder, is caused by the absence of glucose-6-phosphatase (G6Pase) activity. Diagnosis is currently established by demonstrating the lack of G6Pase activity in the patient's liver specimen. Enzymatic diagnosis cannot be performed in chorionic villi or amniocytes as G6Pase is active only in the liver, kidney, and intestinal mucosa. Recent cloning of the G6Pase gene and subsequent identification of the mutations causing GSD 1a have led to the possibility of performing DNA-based diagnosis in chorionic villus samples (CVS) or amniocytes. Here we report the first DNA-based prenatal diagnosis in two families in whom GSD 1a patients were diagnosed. In one Jewish family with a previously identified R83C mutation, single-stranded conformation polymorphism (SSCP) analysis of the DNA extracted from CVS showed a homozygous R83C mutant pattern. As a result, the pregnancy was terminated and the diagnosis was confirmed on DNA analysis of the aborted fetus. In another family of Arabic extraction in which a V166G mutation has been identified in one of the siblings, SSCP analysis performed on DNA extracted from CVS presented the pattern of a normal control. The pregnancy was carried to term and a healthy baby was born. Thus, once mutations causing the disease are identified, prenatal diagnosis of GSD 1a is possible. SSCP analysis of DNA prepared from CVS is reliable, simple and fast, making it a suitable method for prenatal diagnosis.

An open-label, two-period comparative study on pharmacokinetics and safety of a combined ethinylestradiol/gestodene transdermal contraceptive patch.

PubMed

Zhang, Chao; Li, Haiyan; Xiong, Xin; Zhai, Suodi; Wei, Yudong; Zhang, Shuang; Zhang, Yuanyuan; Xu, Lin; Liu, Li

2017-01-01

We investigated the pharmacokinetics and safety profiles of a newly developed combined ethinylestradiol (EE)/gestodene (GSD) transdermal contraceptive patch after a single-dose administration and compared with the market available tablet formulation in healthy adult subjects. An open-label, two-period comparative study was conducted in 12 healthy women volunteers. A single dose of the study combined EE/GE transdermal contraceptive patch and oral tablet (Milunet ® ) were administered. Blood samples at different time points after dose were collected, and concentrations were analyzed. A reliable, highly sensitive and accurate high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC/MS/MS) assay method was developed in this study to determine the plasma concentrations of EE and GSD. Compared to the tablet, the study patch had a significantly decreased maximum plasma concentration ( C max ), extended time to reach the C max and half-life, as well as increased clearance and apparent volume of distribution. The half-lives of EE and GSD of the patch were 3.3 and 2.2 times, respectively, than the half-life of the tablet. The areas under the plasma concentration-time curve (AUCs) of EE and GSD of the patch were 8.0 and 16.2 times, respectively, than the AUC of the tablet. No severe adverse event was observed during the whole study, and the general safety was acceptable. In conclusion, compared to the oral tablet Milunet, the study contraceptive patch was well tolerated and showed potent drug exposure, significant extended half-life and stable drug concentrations.

Recent development and gene therapy for glycogen storage disease type Ia.

PubMed

Chou, Janice Y; Kim, Goo-Young; Cho, Jun-Ho

2017-09-01

Glycogen storage disease type Ia (GSD-Ia) is an autosomal recessive metabolic disorder caused by a deficiency in glucose-6-phosphatase-α (G6Pase-α or G6PC) that is expressed primarily in the liver, kidney, and intestine. G6Pase-α catalyzes the hydrolysis of glucose-6-phosphate (G6P) to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis, and is a key enzyme for endogenous glucose production. The active site of G6Pase-α is inside the endoplasmic reticulum (ER) lumen. For catalysis, the substrate G6P must be translocated from the cytoplasm into the ER lumen by a G6P transporter (G6PT). The functional coupling of G6Pase-α and G6PT maintains interprandial glucose homeostasis. Dietary therapies for GSD-Ia are available, but cannot prevent the long-term complication of hepatocellular adenoma that may undergo malignant transformation to hepatocellular carcinoma. Animal models of GSD-Ia are now available and are being exploited to both delineate the disease more precisely and develop new treatment approaches, including gene therapy.

Dramatic change in a young woman's perception of her diabetes and remarkable reduction in HbA1c after an individual course of Guided Self-Determination

PubMed Central

Zoffmann, Vibeke; Prip, Anne; Christiansen, Anette Wendelboe

2015-01-01

A 24-year-old woman with type 1 diabetes participated in a randomised controlled trial proving effectiveness of a flexible Guided Self-Determination (GSD) intervention. She had for 10 years been living with a complex situation of eating disorder, poor glycaemic control, non-attendance and psychosocial distress. She managed to change her perception of diabetes dramatically and improved her glycaemic control. Considering the complexity of her case, we explored how she achieved these changes. A GSD-trained nurse delivered the intervention, which involves reflection sheets and advanced professional communication. Glycated hemoglobin was reported in the patient's record and an interview conducted by external interviewers was analysed thematically, indicating that a four-stage process of empowerment had taken place: ‘focusing on life prior to numbers’, ‘unpacking a heavy burden’, ‘breaking out of isolation through communication’ and ‘finding strength within oneself’. The article emphasises that GSD works by breaking isolation through communication as an appropriate way to achieve good diabetes control. PMID:26150632

Impact of ghosts on the viscoelastic response of gelatinized corn starch dispersions subjected to small strain deformations.

PubMed

Carrillo-Navas, H; Avila-de la Rosa, G; Gómez-Luría, D; Meraz, M; Alvarez-Ramirez, J; Vernon-Carter, E J

2014-09-22

Corn starch dispersions (5.0% w/w) were gelatinized by heating at 90°C for 20 min using gentle stirring. Under these conditions, ghosts, which are insoluble material with high amylopectin content, were detected by optical microscopy. Strain sweep tests showed that the gelatinized starch dispersions (GSD) exhibited a loss modulus (G″) overshoot at relatively low strains (∼1%). In order to achieve a greater understanding as to the mechanisms giving rise to this uncharacteristic nonlinear response at low strains, very small constant torques (from 0.05 to 0.5 μN m) were applied in the bulk of the GSD with a rotating biconical disc. This resulted in small deformations exhibiting torque-dependent inertio-elastic damped oscillations which were subjected to phenomenological modelling. Inertial effects played an important role in the starch mechanical response. The model parameters varied with the magnitude of constant small applied torque and could be related to microstructural changes of ghosts and to the viscoelastic response of GSD. Copyright © 2014 Elsevier Ltd. All rights reserved.

Evaluation of statistically downscaled GCM output as input for hydrological and stream temperature simulation in the Apalachicola–Chattahoochee–Flint River Basin (1961–99)

USGS Publications Warehouse

Hay, Lauren E.; LaFontaine, Jacob H.; Markstrom, Steven

2014-01-01

The accuracy of statistically downscaled general circulation model (GCM) simulations of daily surface climate for historical conditions (1961–99) and the implications when they are used to drive hydrologic and stream temperature models were assessed for the Apalachicola–Chattahoochee–Flint River basin (ACFB). The ACFB is a 50 000 km2 basin located in the southeastern United States. Three GCMs were statistically downscaled, using an asynchronous regional regression model (ARRM), to ⅛° grids of daily precipitation and minimum and maximum air temperature. These ARRM-based climate datasets were used as input to the Precipitation-Runoff Modeling System (PRMS), a deterministic, distributed-parameter, physical-process watershed model used to simulate and evaluate the effects of various combinations of climate and land use on watershed response. The ACFB was divided into 258 hydrologic response units (HRUs) in which the components of flow (groundwater, subsurface, and surface) are computed in response to climate, land surface, and subsurface characteristics of the basin. Daily simulations of flow components from PRMS were used with the climate to simulate in-stream water temperatures using the Stream Network Temperature (SNTemp) model, a mechanistic, one-dimensional heat transport model for branched stream networks.The climate, hydrology, and stream temperature for historical conditions were evaluated by comparing model outputs produced from historical climate forcings developed from gridded station data (GSD) versus those produced from the three statistically downscaled GCMs using the ARRM methodology. The PRMS and SNTemp models were forced with the GSD and the outputs produced were treated as “truth.” This allowed for a spatial comparison by HRU of the GSD-based output with ARRM-based output. Distributional similarities between GSD- and ARRM-based model outputs were compared using the two-sample Kolmogorov–Smirnov (KS) test in combination with descriptive metrics such as the mean and variance and an evaluation of rare and sustained events. In general, precipitation and streamflow quantities were negatively biased in the downscaled GCM outputs, and results indicate that the downscaled GCM simulations consistently underestimate the largest precipitation events relative to the GSD. The KS test results indicate that ARRM-based air temperatures are similar to GSD at the daily time step for the majority of the ACFB, with perhaps subweekly averaging for stream temperature. Depending on GCM and spatial location, ARRM-based precipitation and streamflow requires averaging of up to 30 days to become similar to the GSD-based output.Evaluation of the model skill for historical conditions suggests some guidelines for use of future projections; while it seems correct to place greater confidence in evaluation metrics which perform well historically, this does not necessarily mean those metrics will accurately reflect model outputs for future climatic conditions. Results from this study indicate no “best” overall model, but the breadth of analysis can be used to give the product users an indication of the applicability of the results to address their particular problem. Since results for historical conditions indicate that model outputs can have significant biases associated with them, the range in future projections examined in terms of change relative to historical conditions for each individual GCM may be more appropriate.

Hepatic glucose-6-phosphatase-α deficiency leads to metabolic reprogramming in glycogen storage disease type Ia.

PubMed

Cho, Jun-Ho; Kim, Goo-Young; Mansfield, Brian C; Chou, Janice Y

2018-04-15

Glycogen storage disease type Ia (GSD-Ia) is caused by a deficiency in glucose-6-phosphatase-α (G6Pase-α or G6PC), a key enzyme in endogenous glucose production. This autosomal recessive disorder is characterized by impaired glucose homeostasis and long-term complications of hepatocellular adenoma/carcinoma (HCA/HCC). We have shown that hepatic G6Pase-α deficiency-mediated steatosis leads to defective autophagy that is frequently associated with carcinogenesis. We now show that hepatic G6Pase-α deficiency also leads to enhancement of hepatic glycolysis and hexose monophosphate shunt (HMS) that can contribute to hepatocarcinogenesis. The enhanced hepatic glycolysis is reflected by increased lactate accumulation, increased expression of many glycolytic enzymes, and elevated expression of c-Myc that stimulates glycolysis. The increased HMS is reflected by increased glucose-6-phosphate dehydrogenase activity and elevated production of NADPH and the reduced glutathione. We have previously shown that restoration of hepatic G6Pase-α expression in G6Pase-α-deficient liver corrects metabolic abnormalities, normalizes autophagy, and prevents HCA/HCC development in GSD-Ia. We now show that restoration of hepatic G6Pase-α expression normalizes both glycolysis and HMS in GSD-Ia. Moreover, the HCA/HCC lesions in L-G6pc-/- mice exhibit elevated levels of hexokinase 2 (HK2) and the M2 isoform of pyruvate kinase (PKM2) which play an important role in aerobic glycolysis and cancer cell proliferation. Taken together, hepatic G6Pase-α deficiency causes metabolic reprogramming, leading to enhanced glycolysis and elevated HMS that along with impaired autophagy can contribute to HCA/HCC development in GSD-Ia. Published by Elsevier Inc.

Sirtuin signaling controls mitochondrial function in glycogen storage disease type Ia.

PubMed

Cho, Jun-Ho; Kim, Goo-Young; Mansfield, Brian C; Chou, Janice Y

2018-05-08

Glycogen storage disease type Ia (GSD-Ia) deficient in glucose-6-phosphatase-α (G6Pase-α) is a metabolic disorder characterized by impaired glucose homeostasis and a long-term complication of hepatocellular adenoma/carcinoma (HCA/HCC). Mitochondrial dysfunction has been implicated in GSD-Ia but the underlying mechanism and its contribution to HCA/HCC development remain unclear. We have shown that hepatic G6Pase-α deficiency leads to downregulation of sirtuin 1 (SIRT1) signaling that underlies defective hepatic autophagy in GSD-Ia. SIRT1 is a NAD + -dependent deacetylase that can deacetylate and activate peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α), a master regulator of mitochondrial integrity, biogenesis, and function. We hypothesized that downregulation of hepatic SIRT1 signaling in G6Pase-α-deficient livers impairs PGC-1α activity, leading to mitochondrial dysfunction. Here we show that the G6Pase-α-deficient livers display defective PGC-1α signaling, reduced numbers of functional mitochondria, and impaired oxidative phosphorylation. Overexpression of hepatic SIRT1 restores PGC-1α activity, normalizes the expression of electron transport chain components, and increases mitochondrial complex IV activity. We have previously shown that restoration of hepatic G6Pase-α expression normalized SIRT1 signaling. We now show that restoration of hepatic G6Pase-α expression also restores PGC-1α activity and mitochondrial function. Finally, we show that HCA/HCC lesions found in G6Pase-α-deficient livers contain marked mitochondrial and oxidative DNA damage. Taken together, our study shows that downregulation of hepatic SIRT1/PGC-1α signaling underlies mitochondrial dysfunction and that oxidative DNA damage incurred by damaged mitochondria may contribute to HCA/HCC development in GSD-Ia.

Are the expected benefits of requirements reuse hampered by distance? An experiment.

PubMed

Carrillo de Gea, Juan M; Nicolás, Joaquín; Fernández-Alemán, José L; Toval, Ambrosio; Idri, Ali

2016-01-01

Software development processes are often performed by distributed teams which may be separated by great distances. Global software development (GSD) has undergone a significant growth in recent years. The challenges concerning GSD are especially relevant to requirements engineering (RE). Stakeholders need to share a common ground, but there are many difficulties as regards the potentially variable interpretation of the requirements in different contexts. We posit that the application of requirements reuse techniques could alleviate this problem through the diminution of the number of requirements open to misinterpretation. This paper presents a reuse-based approach with which to address RE in GSD, with special emphasis on specification techniques, namely parameterised requirements and traceability relationships. An experiment was carried out with the participation of 29 university students enrolled on a Computer Science and Engineering course. Two main scenarios that represented co-localisation and distribution in software development were portrayed by participants from Spain and Morocco. The global teams achieved a slightly better performance than the co-located teams as regards effectiveness , which could be a result of the worse productivity of the global teams in comparison to the co-located teams. Subjective perceptions were generally more positive in the case of the distributed teams ( difficulty , speed and understanding ), with the exception of quality . A theoretical model has been proposed as an evaluation framework with which to analyse, from the point of view of the factor of distance, the effect of requirements specification techniques on a set of performance and perception-based variables. The experiment utilised a new internationalisation requirements catalogue. None of the differences found between co-located and distributed teams were significant according to the outcome of our statistical tests. The well-known benefits of requirements reuse in traditional co-located projects could, therefore, also be expected in GSD projects.

Western Canada study of animal health effects associated with exposure to emissions from oil and natural gas field facilities. Study design and data collection III. Methods of assessing animal exposure to contaminants from the oil and gas industry.

PubMed

Waldner, Cheryl L

2008-01-01

Researchers measured exposure to oil and gas industry emissions in 205 cow-calf herds located in Western Canada. They measured airborne concentrations of sulfur dioxide, hydrogen sulfide, and volatile organic compounds with passive monitors placed in each pasture, wintering, or calving area that contained study animals from the start of the breeding season in the spring of 2001 until June 30, 2002. Researchers continued air monitoring in a subset of herds to the end of the study in fall 2002. Each sampling device was exposed for 1 month and then shipped to the laboratory for analysis. New samplers were installed and the shelters relocated, as necessary, to follow the movements of herd-management groups between pastures. Researchers linked the results of the air-monitoring analysis to individual animals for the relevant month. For the 205 herds examined at pregnancy testing in 2001, monthly mean exposures on the basis of all available data were as follows: sulfur dioxide, geometric mean (GM)=0.5 ppb, geometric standard deviation (GSD)=2.2; hydrogen sulfide, GM=0.14 ppb, GSD=2.3; benzene, GM=0.247 microg/m3, GSD=2.5; and toluene, GM=0.236 microg/m3, GSD=2.7. Benzene and toluene were surrogates for volatile organic compound exposure. In addition to passive measurements of air quality, researchers obtained data from provincial regulatory agencies on the density of oil and gas field facilities and on flaring and venting from the surrounding facilities. They developed the data into additional measures of exposure that were linked to each animal at each location for each month of the study.

Can f 1 levels in hair and homes of different dog breeds: lack of evidence to describe any dog breed as hypoallergenic.

PubMed

Vredegoor, Doris W; Willemse, Ton; Chapman, Martin D; Heederik, Dick J J; Krop, Esmeralda J M

2012-10-01

Certain dog breeds are described and marketed as being "hypoallergenic" on the basis of anecdotal reports that these dogs are better tolerated by patients allergic to dogs. These observations were investigated by comparing Can f 1 (major dog [Canis familiaris] allergen) levels in hair and coat samples and in the home environment of various hypoallergenic (Labradoodle, Poodle, Spanish Waterdog, and Airedale terrier) and non-hypoallergenic dogs (Labrador retriever and a control group). Hair and coat samples were obtained from dogs, and settled floor and airborne dust samples were taken from the dogs' homes. Can f 1 concentrations were measured by using ELISA, and results were analyzed by using multiple linear regression analyses. Significantly higher Can f 1 concentrations were found in hair and coat samples of hypoallergenic dogs (n = 196, geometric mean [GM], 2.26 μg/g, geometric standard deviation [GSD], 0.73, and GM, 27.04 μg/g, GSD, 0.57, respectively) than of non-hypoallergenic dogs (n = 160, GM, 0.77 μg/g, GSD, 0.71, and GM, 12.98 μg/g, GSD, 0.76, respectively). Differences between breeds were small, relative to the variability within a breed. Can f 1 levels in settled floor dust samples were lower for Labradoodles, but no differences were found between the other groups. No differences in airborne levels were found between breeds. So-called hypoallergenic dogs had higher Can f 1 levels in hair and coat samples than did control breeds. These differences did not lead to higher levels of environmental exposure to dog allergens. There is no evidence for the classification of certain dog breeds as being "hypoallergenic." Copyright © 2012 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

Fibromyalgia in 300 adult index patients with primary immunodeficiency.

PubMed

Barton, James C; Bertoli, Luigi F; Barton, Jackson C; Acton, Ronald T

2017-01-01

We sought to determine the prevalence and clinical and laboratory associations of fibromyalgia in adults with primary immunodeficiency (immunoglobulin (Ig) G subclass deficiency (IgGSD) and common variable immunodeficiency (CVID). We performed a retrospective analysis of these observations in 300 non-Hispanic white adult index patients with recurrent/severe respiratory tract infections and IgGSD or CVID: age; sex; IgGSD; fibromyalgia; chronic fatigue; autoimmune conditions (ACs); interstitial cystitis (IC); diabetes; body mass index; serum Ig isotypes; blood lymphocytes and subsets; and human leukocyte antigen (HLA)-A and -B types and haplotypes. We performed univariate comparisons, logistic multivariable regressions, and an analysis of covariance. Mean age was 49 ± 12 (standard deviation) y. There were 246 women (82.0%). IgGSD was diagnosed in 276 patients (92.0%). Fifty-six patients had fibromyalgia (18.7%; female:male 13:1). Other characteristics included: chronic fatigue, 63.0%; aggregate ACs, 35.3%; Sjögren's syndrome, 8.0%; IC, 3.0%; diabetes, 10.3%; and HLA-A*29, B*44 positivity, 9.7%. Prevalences of female sex; chronic fatigue; IC; and HLA-A*29, B*44 positivity were greater in patients with fibromyalgia. Logistic regression on fibromyalgia revealed three positive associations: chronic fatigue (p=0.0149; odds ratio 2.6 [95% confidence interval 1.2, 5.6]); Sjögren's syndrome (p=0.0004; 5.2 [2.1, 13.2]); and IC (p=0.0232; 5.7 [1.3, 25.7]). In an analysis of covariance, there were significant interactions of chronic fatigue, Sjögren's syndrome, and interstitial cystitis on fibromyalgia. Fibromyalgia is common in non-Hispanic white adult index patients with primary immunodeficiency, especially women. Chronic fatigue, Sjögren's syndrome, and IC are significantly associated with fibromyalgia after adjustment for other independent variables.

Particle size distribution of mainstream tobacco and marijuana smoke. Analysis using the electrical aerosol analyzer.

PubMed

Anderson, P J; Wilson, J D; Hiller, F C

1989-07-01

Accurate measurement of cigarette smoke particle size distribution is important for estimation of lung deposition. Most prior investigators have reported a mass median diameter (MMD) in the size range of 0.3 to 0.5 micron, with a small geometric standard deviation (GSD), indicating few ultrafine (less than 0.1 micron) particles. A few studies, however, have suggested the presence of ultrafine particles by reporting a smaller count median diameter (CMD). Part of this disparity may be due tot he inefficiency to previous sizing methods in measuring ultrafine size range, to evaluate size distribution of smoke from standard research cigarettes, commercial filter cigarettes, and from marijuana cigarettes with different delta 9-tetrahydrocannabinol contents. Four 35-cm3, 2-s puffs were generated at 60-s intervals, rapidly diluted, and passed through a charge neutralizer and into a 240-L chamber. Size distribution for six cigarettes of each type was measured, CMD and GSD were determined from a computer-generated log probability plot, and MMD was calculated. The size distribution parameters obtained were similar for all cigarettes tested, with an average CMD of 0.1 micron, a MMD of 0.38 micron, and a GSD of 2.0. The MMD found using the EAA is similar to that previously reported, but the CMD is distinctly smaller and the GSD larger, indicating the presence of many more ultrafine particles. These results may explain the disparity of CMD values found in existing data. Ultrafine particles are of toxicologic importance because their respiratory tract deposition is significantly higher than for particles 0.3 to 0.5 micron and because their large surface area facilitates adsorption and delivery of potentially toxic gases to the lung.

Multi sensor satellite imagers for commercial remote sensing

NASA Astrophysics Data System (ADS)

Cronje, T.; Burger, H.; Du Plessis, J.; Du Toit, J. F.; Marais, L.; Strumpfer, F.

2005-10-01

This paper will discuss and compare recent refractive and catodioptric imager designs developed and manufactured at SunSpace for Multi Sensor Satellite Imagers with Panchromatic, Multi-spectral, Area and Hyperspectral sensors on a single Focal Plane Array (FPA). These satellite optical systems were designed with applications to monitor food supplies, crop yield and disaster monitoring in mind. The aim of these imagers is to achieve medium to high resolution (2.5m to 15m) spatial sampling, wide swaths (up to 45km) and noise equivalent reflectance (NER) values of less than 0.5%. State-of-the-art FPA designs are discussed and address the choice of detectors to achieve these performances. Special attention is given to thermal robustness and compactness, the use of folding prisms to place multiple detectors in a large FPA and a specially developed process to customize the spectral selection with the need to minimize mass, power and cost. A refractive imager with up to 6 spectral bands (6.25m GSD) and a catodioptric imager with panchromatic (2.7m GSD), multi-spectral (6 bands, 4.6m GSD), hyperspectral (400nm to 2.35μm, 200 bands, 15m GSD) sensors on the same FPA will be discussed. Both of these imagers are also equipped with real time video view finding capabilities. The electronic units could be subdivided into the Front-End Electronics and Control Electronics with analogue and digital signal processing. A dedicated Analogue Front-End is used for Correlated Double Sampling (CDS), black level correction, variable gain and up to 12-bit digitizing and high speed LVDS data link to a mass memory unit.

Putative Independent Evolutionary Reversals from Genotypic to Temperature-Dependent Sex Determination are Associated with Accelerated Evolution of Sex-Determining Genes in Turtles.

PubMed

Literman, Robert; Burrett, Alexandria; Bista, Basanta; Valenzuela, Nicole

2018-01-01

The evolutionary lability of sex-determining mechanisms across the tree of life is well recognized, yet the extent of molecular changes that accompany these repeated transitions remain obscure. Most turtles retain the ancestral temperature-dependent sex determination (TSD) from which multiple transitions to genotypic sex determination (GSD) occurred independently, and two contrasting hypotheses posit the existence or absence of reversals back to TSD. Here we examined the molecular evolution of the coding regions of a set of gene regulators involved in gonadal development in turtles and several other vertebrates. We found slower molecular evolution in turtles and crocodilians compared to other vertebrates, but an acceleration in Trionychia turtles and at some phylogenetic branches demarcating major taxonomic diversification events. Of all gene classes examined, hormone signaling genes, and Srd5a1 in particular, evolve faster in many lineages and especially in turtles. Our data show that sex-linked genes do not follow a ubiquitous nor uniform pattern of molecular evolution. We then evaluated turtle nucleotide and protein evolution under two evolutionary hypotheses with or without GSD-to-TSD reversals, and found that when GSD-to-TSD reversals are considered, all transitional branches irrespective of direction, exhibit accelerated molecular evolution of nucleotide sequences, while GSD-to-TSD transitional branches also show acceleration in protein evolution. Significant changes in predicted secondary structure that may affect protein function were identified in three genes that exhibited hastened evolution in turtles compared to other vertebrates or in transitional versus non-transitional branches within turtles, rendering them candidates for a key role during SDM evolution in turtles.

Effect of a Low-Dose Contraceptive Patch on Efficacy, Bleeding Pattern, and Safety

PubMed Central

Wiegratz, Inka; Bassol, Susana; Weisberg, Edith; Mellinger, Uwe

2014-01-01

This Phase III, uncontrolled, open-label, multicenter study was conducted to investigate the contraceptive efficacy, bleeding pattern, and cycle control of a novel once-a-week contraceptive patch, delivering low-dose ethinyl estradiol (EE) and gestodene (GSD) at the same systemic exposure seen after oral administration of a combined oral contraceptive containing 0.02 mg EE/0.06 mg GSD. Participants were women aged 18 to 35 years, all of whom received the EE/GSD patch for 13 cycles each of 21 treatment days (one patch per week for 3 weeks) followed by a 7-day, patch-free interval. The primary efficacy variable was the occurrence of unintended pregnancies during the study period as assessed by life table analysis and the Pearl Index. Secondary efficacy variables were days with bleeding during four 90-day reference periods and during 1 treatment year, bleeding pattern, and cycle control. The Kaplan-Meier probability of contraceptive protection after 364 treatment days was 98.8% and the adjusted Pearl Index was 0.81. The percentage of participants with intracyclic bleeding/spotting decreased over time, from 11.4% to 6.8% in cycles 1 and 12, respectively. Almost all participants (range: 90.8%-97.6%) experienced withdrawal bleeding across the study period. Compliance was very high (mean: 97.9%; median: 100%). The most frequent adverse events were headache (9.5%) and application site reaction (8.5%); no clinically significant safety concerns were observed. Results suggest the EE/GSD patch is highly effective in preventing pregnancy. Menstrual bleeding pattern was favorable and within the ranges expected of a healthy female population. The patch was well tolerated and treatment compliance was high. PMID:24784719

Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber. I. Quantitation of lung and pleural fiber burdens.

PubMed

Gelzleichter, T R; Bermudez, E; Mangum, J B; Wong, B A; Everitt, J I; Moss, O R

1996-03-01

The pleura is an important target tissue of fiber-induced disease, although it is not known whether fibers must be in direct contact with pleural cells to exert pathologic effects. In the present study, we determined the kinetics of fiber movement into pleural tissues of rats following inhalation of RCF-1, a ceramic fiber previously shown to induce neoplasms in the lung and pleura of rats. Male Fischer 344 rats were exposed by nose-only inhalation to RCF-1 at 89 mg/m3 (2645 WHO fibers/cc), 6 hr/day for 5 consecutive days. On Days 5 and 32, thoracic tissues were analyzed to determine pulmonary and pleural fiber burdens. Mean fiber counts were 22 x 10(6)/lung (25 x 10(3)/pleura) at Day 5 and 18 x 10(6)/lung (16 x 10(3)/pleura) at Day 32. Similar geometric mean lengths (GML) and diameters (GMD) of pulmonary fiber burdens were observed at both time points. Values were 5 microns for GML (geometric standard deviation GSD approximately 2.3) and 0.3 micron for GMD (GSD approximately 1.9), with correlations between length and diameter (tau) of 0.2-0.3. Size distributions of pleural fiber burdens at both time points were approximately 1.5 microns GML (GSD approximately 2.0) and 0.09 micron GMD (GSD approximately 1.5; tau approximately 0.2-0.5). Few fibers longer than 5 microns were observed at either time point. These findings demonstrate that fibers can rapidly translocate to pleural tissues. However, only short, thin (< 5 microns in length) fibers could be detected over the 32-day time course of the experiment.

Exposure to grain dust and microbial components in the Norwegian grain and compound feed industry.

PubMed

Halstensen, Anne Straumfors; Heldal, Kari Kulvik; Wouters, Inge M; Skogstad, Marit; Ellingsen, Dag G; Eduard, Wijnand

2013-11-01

The aim of this study was to extensively characterize grain workers' personal exposure during work in Norwegian grain elevators and compound feed mills, to identify differences in exposures between the workplaces and seasons, and to study the correlations between different microbial components. Samples of airborne dust (n = 166) were collected by full-shift personal sampling during work in 20 grain elevators and compound feed mills during one autumn season and two winter seasons. The personal exposure to grain dust, endotoxins, β-1→3-glucans, bacteria, and fungal spores was quantified. Correlations between dust and microbial components and differences between workplaces and seasons were investigated. Determinants of endotoxin and β-1→3-glucan exposure were evaluated by linear mixed-effect regression modeling. The workers were exposed to an overall geometric mean of 1.0mg m(-3) inhalable grain dust [geometric standard deviation (GSD) = 3.7], 628 endotoxin units m(-3) (GSD = 5.9), 7.4 µg m(-3) of β-1→3-glucan (GSD = 5.6), 21 × 10(4) bacteria m(-3) (GSD = 7.9) and 3.6 × 10(4) fungal spores m(-3) (GSD = 3.4). The grain dust exposure levels were similar across workplaces and seasons, but the microbial content of the grain dust varied substantially between workplaces. Exposure levels of all microbial components were significantly higher in grain elevators compared with all other workplaces. The grain dust exposure was significantly correlated (Pearson's r) with endotoxin (rp = 0.65), β-1→3-glucan (rp = 0.72), bacteria (rp = 0.44) and fungal spore (rp = 0.48) exposure, whereas the explained variances were strongly dependent on the workplace. Bacteria, grain dust, and workplace were important determinants for endotoxin exposure, whereas fungal spores, grain dust, and workplace were important determinants for β-1→3-glucan exposure. Although the workers were exposed to a relatively low mean dust level, the microbial exposure was high. Furthermore, the exposure levels of microbial components varied between workplaces although the dust levels were similar. We therefore recommend that exposure levels at different workplaces should be assessed separately and a task-based assessment should be done for detailed evaluation of efficient dust-reducing measures. The microbial content and knowledge of health effects of the microbial components should be considered in health risk evaluations of these workplaces.

Group 3 medulloblastoma in a patient with a GYS2 germline mutation and glycogen storage disease 0a.

PubMed

Holsten, Till; Tsiakas, Konstantinos; Kordes, Uwe; Bison, Brigitte; Pietsch, Torsten; Rutkowski, Stefan; Santer, René; Schüller, Ulrich

2018-03-01

Glycogen storage disease (GSD) 0a is a rare congenital metabolic disease with symptoms in infancy and childhood caused by biallelic GYS2 germline variants. A predisposition to cancer has not been described yet. We report here a boy with GSD 0a, who developed a malignant brain tumor at the age of 4.5 years. The tumor was classified as a group 3 medulloblastoma, and the patient died from cancer 27 months after initial tumor diagnosis. This case appears interesting as group 3 medulloblastoma is so far not known to arise in hereditary syndromes and the biology of sporadic group 3 medulloblastoma is largely unknown.

Dental disease indices and caries-related microflora in children with glycogen storage disease.

PubMed

Kidd, S A; Rademeyer, C; Roberts, G J; Lee, P J; Lucas, V S

2002-01-01

To establish the levels of dental caries, bacterial dental plaque, gingivitis and caries-related microflora in children with glycogen storage disease (GSD). Patients with GSD are treated with regular intakes of glucose polymer and uncooked cornstarch to prevent hypoglycaemia. Dental health data are scarce. The study group comprised 21 children with GSD attending the Great Ormond Street Hospital for Children. These included the number of decayed, missing and filled teeth, and surfaces in both the primary and permanent dentitions, plaque and gingivitis scores. Both plaque and saliva were collected from each child and cultured for Mutans streptococci, Lactobacilli and Candida. The study group included 13 boys and eight girls, aged from 2.7 to 15.5 years. Four of the 21 children had some caries experience. The mean dmft was 0.5 and the mean DMFT, 0.06. Mean plaque and gingivitis scores were 4.8 and 5.9, respectively, for plaque and gingivitis adjacent to the primary teeth, and 11.6 and 12 for those related to permanent teeth. Only a small proportion of the children had caries experience but most were found to have plaque associated with both primary and permanent teeth. Preventive care should be targeted to improve plaque control thus minimizing the risk of developing periodontal disease as adults.

Use of Vertical Aerial Images for Semi-Oblique Mapping

NASA Astrophysics Data System (ADS)

Poli, D.; Moe, K.; Legat, K.; Toschi, I.; Lago, F.; Remondino, F.

2017-05-01

The paper proposes a methodology for the use of the oblique sections of images from large-format photogrammetric cameras, by exploiting the effect of the central perspective geometry in the lateral parts of the nadir images ("semi-oblique" images). The point of origin of the investigation was the execution of a photogrammetric flight over Norcia (Italy), which was seriously damaged after the earthquake of 30/10/2016. Contrary to the original plan of oblique acquisitions, the flight was executed on 15/11/2017 using an UltraCam Eagle camera with focal length 80 mm, and combining two flight plans, rotated by 90º ("crisscross" flight). The images (GSD 5 cm) were used to extract a 2.5D DSM cloud, sampled to a XY-grid size of 2 GSD, a 3D point clouds with a mean spatial resolution of 1 GSD and a 3D mesh model at a resolution of 10 cm of the historic centre of Norcia for a quantitative assessment of the damages. From the acquired nadir images the "semi-oblique" images (forward, backward, left and right views) could be extracted and processed in a modified version of GEOBLY software for measurements and restitution purposes. The potential of such semi-oblique image acquisitions from nadir-view cameras is hereafter shown and commented.

Reliability of concentrations of organophosphate pesticide metabolites in serial urine specimens from pregnancy in the Generation R Study.

PubMed

Spaan, Suzanne; Pronk, Anjoeka; Koch, Holger M; Jusko, Todd A; Jaddoe, Vincent W V; Shaw, Pamela A; Tiemeier, Henning M; Hofman, Albert; Pierik, Frank H; Longnecker, Matthew P

2015-05-01

The widespread use of organophosphate (OP) pesticides has resulted in ubiquitous exposure in humans, primarily through their diet. Exposure to OP pesticides may have adverse health effects, including neurobehavioral deficits in children. The optimal design of new studies requires data on the reliability of urinary measures of exposure. In the present study, urinary concentrations of six dialkyl phosphate (DAP) metabolites, the main urinary metabolites of OP pesticides, were determined in 120 pregnant women participating in the Generation R Study in Rotterdam. Intra-class correlation coefficients (ICCs) across serial urine specimens taken at <18, 18-25, and >25 weeks of pregnancy were determined to assess reliability. Geometric mean total DAP metabolite concentrations were 229 (GSD 2.2), 240 (GSD 2.1), and 224 (GSD 2.2) nmol/g creatinine across the three periods of gestation. Metabolite concentrations from the serial urine specimens in general correlated moderately. The ICCs for the six DAP metabolites ranged from 0.14 to 0.38 (0.30 for total DAPs), indicating weak to moderate reliability. Although the DAP metabolite levels observed in this study are slightly higher and slightly more correlated than in previous studies, the low to moderate reliability indicates a high degree of within-person variability, which presents challenges for designing well-powered epidemiological studies.

Comparison of geometrical shock dynamics and kinematic models for shock-wave propagation

NASA Astrophysics Data System (ADS)

Ridoux, J.; Lardjane, N.; Monasse, L.; Coulouvrat, F.

2018-03-01

Geometrical shock dynamics (GSD) is a simplified model for nonlinear shock-wave propagation, based on the decomposition of the shock front into elementary ray tubes. Assuming small changes in the ray tube area, and neglecting the effect of the post-shock flow, a simple relation linking the local curvature and velocity of the front, known as the A{-}M rule, is obtained. More recently, a new simplified model, referred to as the kinematic model, was proposed. This model is obtained by combining the three-dimensional Euler equations and the Rankine-Hugoniot relations at the front, which leads to an equation for the normal variation of the shock Mach number at the wave front. In the same way as GSD, the kinematic model is closed by neglecting the post-shock flow effects. Although each model's approach is different, we prove their structural equivalence: the kinematic model can be rewritten under the form of GSD with a specific A{-}M relation. Both models are then compared through a wide variety of examples including experimental data or Eulerian simulation results when available. Attention is drawn to the simple cases of compression ramps and diffraction over convex corners. The analysis is completed by the more complex cases of the diffraction over a cylinder, a sphere, a mound, and a trough.

The dragon lizard Pogona vitticeps has ZZ/ZW micro-sex chromosomes.

PubMed

Ezaz, Tariq; Quinn, Alexander E; Miura, Ikuo; Sarre, Stephen D; Georges, Arthur; Marshall Graves, Jennifer A

2005-01-01

The bearded dragon, Pogona vitticeps (Agamidae: Reptilia) is an agamid lizard endemic to Australia. Like crocodilians and many turtles, temperature-dependent sex determination (TSD) is common in agamid lizards, although many species have genotypic sex determination (GSD). P. vitticeps is reported to have GSD, but no detectable sex chromosomes. Here we used molecular cytogenetic and differential banding techniques to reveal sex chromosomes in this species. Comparative genomic hybridization (CGH), GTG- and C-banding identified a highly heterochromatic microchromosome specific to females, demonstrating female heterogamety (ZZ/ZW) in this species. We isolated the P. vitticeps W chromosome by microdissection, re-amplified the DNA and used it to paint the W. No unpaired bivalents were detected in male synaptonemal complexes at meiotic pachytene, confirming male homogamety. We conclude that P. vitticeps has differentiated previously unidentifable W and Z micro-sex chromosomes, the first to be demonstrated in an agamid lizard. Our finding implies that heterochromatinization of the heterogametic chromosome occurred during sex chromosome differentiation in this species, as is the case in some lizards and many snakes, as well as in birds and mammals. Many GSD reptiles with cryptic sex chromosomes may also prove to have micro-sex chromosomes. Reptile microchromosomes, long dismissed as non-functional minutiae and often omitted from karyotypes, therefore deserve closer scrutiny with new and more sensitive techniques.

Early growth and development impairments in patients with ganglioside GM3 synthase deficiency.

PubMed

Wang, H; Wang, A; Wang, D; Bright, A; Sency, V; Zhou, A; Xin, B

2016-05-01

Ganglioside GM3 synthase is a key enzyme involved in the biosynthesis of gangliosides. GM3 synthase deficiency (GSD) causes a complete absence of GM3 and all downstream biosynthetic derivatives. The individuals affected by this disorder manifest severe irritability, intractable seizures and profound intellectual disability. However, we have found that most newborns seem symptom-free for a period of time after birth. In order to further understand the onset of the disease, we investigated the early growth and development of patients with this condition through this study. We compared 37 affected individuals with their normal siblings and revealed that all children with GSD had relatively normal intrauterine growth and development, as their weight, length and head circumference were similar to their normal siblings at birth. However, the disease progresses quickly after birth and causes significant constitutional impairments of growth and development by 6 months of age. Neither breastfeeding nor gastrostomy tube placement made significant difference on growth and development as all groups of patients showed the similar pattern. We conclude that GSD causes significant postnatal growth and developmental impairments and the amount of gangliosides in breast milk and general nutritional intervention do not seem to alter these outcomes. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Geometric and Radiometric Evaluation of Rasat Images

NASA Astrophysics Data System (ADS)

Cam, Ali; Topan, Hüseyin; Oruç, Murat; Özendi, Mustafa; Bayık, Çağlar

2016-06-01

RASAT, the second remote sensing satellite of Turkey, was designed and assembled, and also is being operated by TÜBİTAK Uzay (Space) Technologies Research Institute (Ankara). RASAT images in various levels are available free-of-charge via Gezgin portal for Turkish citizens. In this paper, the images in panchromatic (7.5 m GSD) and RGB (15 m GSD) bands in various levels were investigated with respect to its geometric and radiometric characteristics. The first geometric analysis is the estimation of the effective GSD as less than 1 pixel for radiometrically processed level (L1R) of both panchromatic and RGB images. Secondly, 2D georeferencing accuracy is estimated by various non-physical transformation models (similarity, 2D affine, polynomial, affine projection, projective, DLT and GCP based RFM) reaching sub-pixel accuracy using minimum 39 and maximum 52 GCPs. The radiometric characteristics are also investigated for 8 bits, estimating SNR between 21.8-42.2, and noise 0.0-3.5 for panchromatic and MS images for L1R when the sea is masked to obtain the results for land areas. The analysis show that RASAT images satisfies requirements for various applications. The research is carried out in Zonguldak test site which is mountainous and partly covered by dense forest and urban areas.

Determining mutations in G6PC and SLC37A4 genes in a sample of Brazilian patients with glycogen storage disease types Ia and Ib.

PubMed

Carlin, Marcelo Paschoalete; Scherrer, Daniel Zanetti; De Tommaso, Adriana Maria Alves; Bertuzzo, Carmen Silvia; Steiner, Carlos Eduardo

2013-12-01

Glycogen storage disease (GSD) comprises a group of autosomal recessive disorders characterized by deficiency of the enzymes that regulate the synthesis or degradation of glycogen. Types Ia and Ib are the most prevalent; while the former is caused by deficiency of glucose-6-phosphatase (G6Pase), the latter is associated with impaired glucose-6-phosphate transporter, where the catalytic unit of G6Pase is located. Over 85 mutations have been reported since the cloning of G6PC and SLC37A4 genes. In this study, twelve unrelated patients with clinical symptoms suggestive of GSDIa and Ib were investigated by using genetic sequencing of G6PC and SLC37A4 genes, being three confirmed as having GSD Ia, and two with GSD Ib. In seven of these patients no mutations were detected in any of the genes. Five changes were detected in G6PC, including three known point mutations (p.G68R, p.R83C and p.Q347X) and two neutral mutations (c.432G > A and c.1176T > C). Four changes were found in SLC37A4: a known point mutation (p.G149E), a novel frameshift insertion (c.1338_1339insT), and two neutral mutations (c.1287G > A and c.1076-28C > T). The frequency of mutations in our population was similar to that observed in the literature, in which the mutation p.R83C is also the most frequent one. Analysis of both genes should be considered in the investigation of this condition. An alternative explanation to the negative results in this molecular study is the possibility of a misdiagnosis. Even with a careful evaluation based on laboratory and clinical findings, overlap with other types of GSD is possible, and further molecular studies should be indicated.

Constructing a Database of Similar Exposure Groups: The Application of the Exporisq-HAP Database from 1995 to 2015.

PubMed

Petit, Pascal; Bicout, Dominique J; Persoons, Renaud; Bonneterre, Vincent; Barbeau, Damien; Maître, Anne

2017-05-01

Similar exposure groups (SEGs) are needed to reliably assess occupational exposures and health risks. However, the construction of SEGs can turn out to be rather challenging because of the multifactorial variability of exposures. The objective of this study is to put forward a semi-empirical approach developed to construct and implement a SEG database for exposure assessments. An occupational database of airborne levels of polycyclic aromatic hydrocarbons (PAHs) was used as an illustrative and working example. The approach that was developed consisted of four steps. The first three steps addressed the construction and implementation of the occupational Exporisq-HAP database (E-HAP). E-HAP was structured into three hierarchical levels of exposure groups, each of which was based on exposure determinants, along 16 dimensions that represented the sampled PAHs. A fourth step was implemented to identify and generate SEGs using the geometric standard deviation (GSD) of PAH concentrations. E-HAP was restructured into 16 (for 16 sampled PAHs) 3 × 3 matrices: three hierarchical levels of description versus three degrees of dispersion, which included low (the SEG database: GSD ≤ 3), medium (3 < GSD ≤ 6), and high (GSD > 6). Benzo[a]pyrene (BaP) was the least dispersed particulate PAH with 41.5% of groups that could be considered as SEGs, 48.5% of groups of medium dispersion, and only 8% with high dispersion. These results were comparable for BaP, BaP equivalent toxic, or the sum of all carcinogenic PAHs but were different when individual gaseous PAHs or ∑PAHG were chosen. Within the framework of risk assessment, such an approach, based on groundwork studies, allows for both the construction of an SEG database and the identification of exposure groups that require improvements in either the description level or the homogeneity degree toward SEG. © The Author 2017. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

In Vivo Zinc Finger Nuclease-mediated Targeted Integration of a Glucose-6-phosphatase Transgene Promotes Survival in Mice With Glycogen Storage Disease Type IA

PubMed Central

Landau, Dustin J; Brooks, Elizabeth Drake; Perez-Pinera, Pablo; Amarasekara, Hiruni; Mefferd, Adam; Li, Songtao; Bird, Andrew; Gersbach, Charles A; Koeberl, Dwight D

2016-01-01

Glycogen storage disease type Ia (GSD Ia) is caused by glucose-6-phosphatase (G6Pase) deficiency in association with severe, life-threatening hypoglycemia that necessitates lifelong dietary therapy. Here we show that use of a zinc-finger nuclease (ZFN) targeted to the ROSA26 safe harbor locus and a ROSA26-targeting vector containing a G6PC donor transgene, both delivered with adeno-associated virus (AAV) vectors, markedly improved survival of G6Pase knockout (G6Pase-KO) mice compared with mice receiving the donor vector alone (P < 0.04). Furthermore, transgene integration has been confirmed by sequencing in the majority of the mice treated with both vectors. Targeted alleles were 4.6-fold more common in livers of mice with GSD Ia, as compared with normal littermates, at 8 months following vector administration (P < 0.02). This suggests a selective advantage for vector-transduced hepatocytes following ZFN-mediated integration of the G6Pase vector. A short-term experiment also showed that 3-month-old mice receiving the ZFN had significantly-improved biochemical correction, in comparison with mice that received the donor vector alone. These data suggest that the use of ZFNs to drive integration of G6Pase at a safe harbor locus might improve vector persistence and efficacy, and lower mortality in GSD Ia. PMID:26865405

Reliability of concentrations of organophosphate pesticide metabolites in serial urine specimens from pregnancy in the Generation R study

PubMed Central

Spaan, Suzanne; Pronk, Anjoeka; Koch, Holger M.; Jusko, Todd A.; Jaddoe, Vincent W.V.; Shaw, Pamela A.; Tiemeier, Henning M.; Hofman, Albert; Pierik, Frank H.; Longnecker, Matthew P.

2014-01-01

The widespread use of organophosphate (OP) pesticides has resulted in ubiquitous exposure in humans, primarily through their diet. Exposure to OP pesticides may have adverse health effects, including neurobehavioral deficits in children. The optimal design of new studies requires data on the reliability of urinary measures of exposure. In the present study, urinary concentrations of six dialkyl phosphate (DAP) metabolites, the main urinary metabolites of OP pesticides, were determined in 120 pregnant women participating in the Generation R Study in Rotterdam. Intra-class correlation coefficients (ICCs) across serial urine specimens taken at <18, 18–25, and >25 weeks of pregnancy were determined to assess reliability. Geometric mean total DAP metabolite concentrations were 229 (GSD 2.2), 240 (GSD 2.1), and 224 (GSD 2.2) nmol/g creatinine across the three periods of gestation. Metabolite concentrations from the serial urine specimens in general correlated moderately. The ICCs for the six DAP metabolites ranged from 0.14 to 0.38 (0.30 for total DAPs), indicating weak to moderate reliability. Although the DAP metabolite levels observed in this study are slightly higher and slightly more correlated than in previous studies, the low to moderate reliability indicates a high degree of within-person variability, which presents challenges for designing well-powered epidemiologic studies. PMID:25515376

Glycogen branching enzyme (GBE1) mutation causing equine glycogen storage disease IV.

PubMed

Ward, Tara L; Valberg, Stephanie J; Adelson, David L; Abbey, Colette A; Binns, Matthew M; Mickelson, James R

2004-07-01

Comparative biochemical and histopathological evidence suggests that a deficiency in the glycogen branching enzyme, encoded by the GBE1 gene, is responsible for a recently identified recessive fatal fetal and neonatal glycogen storage disease (GSD) in American Quarter Horses termed GSD IV. We have now derived the complete GBE1 cDNA sequences for control horses and affected foals, and identified a C to A substitution at base 102 that results in a tyrosine (Y) to stop (X) mutation in codon 34 of exon 1. All 11 affected foals were homozygous for the X34 allele, their 11 available dams and sires were heterozygous, and all 16 control horses were homozygous for the Y34 allele. The previous findings of poorly branched glycogen, abnormal polysaccharide accumulation, lack of measurable GBE1 enzyme activity and immunodetectable GBE1 protein, coupled with the present observation of abundant GBE1 mRNA in affected foals, are all consistent with the nonsense mutation in the 699 amino acid GBE1 protein. The affected foal pedigrees have a common ancestor and contain prolific stallions that are likely carriers of the recessive X34 allele. Defining the molecular basis of equine GSD IV will allow for accurate DNA testing and the ability to prevent occurrence of this devastating disease affecting American Quarter Horses and related breeds.

Effect of oral contraceptives on markers of hyperandrogenism and SHBG in women with polycystic ovary syndrome.

PubMed

De Leo, Vincenzo; Di Sabatino, Alessandra; Musacchio, Maria C; Morgante, Giuseppe; Scolaro, Valeria; Cianci, Antonio; Petraglia, Felice

2010-09-01

This randomized study's aim was to compare the effect of four oral contraceptives (OCs) containing 30 mcg of ethinylestradiol (EE) and different progestogens [drospirenone, (DRSP), chlormadinone acetate (CMA), desogestrel (DSG), gestodene (GSD)] on biochemical and hormonal parameters of hyperandrogenism and sex hormone-binding globulin (SHBG) in women with polycystic ovary syndrome (PCOS). Forty women with PCOS (age 16-35 years) were recruited and randomly assigned to one of four treatment groups of 10 women each, treated, respectively, with 3 mg DRSP/30 mcg EE (Yasmin, Bayer Shering), 2 mg CMA/30 mcg EE (Belara, Grunenthal), 75 mcg GSD/30 mcg EE (Minulet, Wyeth Lederle) and 150 mcg DSG/30 mcg EE (Practil 21, Organon Italia). Blood samples were obtained on day 6-8 of the control cycle and day 6-8 of the third treatment cycle for assay of the following hormones: androsteredione (A), total testosterone (T), free T, SHBG, dehydroepiandrosterone sulphate (DHEAS). In all groups, mean concentrations of free T, total T and A dropped by 40-60%, and concentrations of DHEAS dropped by 20-50%. Formulations with DRSP and CMA caused a greater reduction of androgens and a progressive increase in serum concentrations of SHBG than those with DSG and GSD. Clinical studies need to be performed to determine effects of these OCs upon clinical signs of hyperandrogenism. Copyright 2010 Elsevier Inc. All rights reserved.

The First Attempt at Non-Linear in Silico Prediction of Sampling Rates for Polar Organic Chemical Integrative Samplers (POCIS)

PubMed Central

2016-01-01

Modeling and prediction of polar organic chemical integrative sampler (POCIS) sampling rates (Rs) for 73 compounds using artificial neural networks (ANNs) is presented for the first time. Two models were constructed: the first was developed ab initio using a genetic algorithm (GSD-model) to shortlist 24 descriptors covering constitutional, topological, geometrical and physicochemical properties and the second model was adapted for Rs prediction from a previous chromatographic retention model (RTD-model). Mechanistic evaluation of descriptors showed that models did not require comprehensive a priori information to predict Rs. Average predicted errors for the verification and blind test sets were 0.03 ± 0.02 L d–1 (RTD-model) and 0.03 ± 0.03 L d–1 (GSD-model) relative to experimentally determined Rs. Prediction variability in replicated models was the same or less than for measured Rs. Networks were externally validated using a measured Rs data set of six benzodiazepines. The RTD-model performed best in comparison to the GSD-model for these compounds (average absolute errors of 0.0145 ± 0.008 L d–1 and 0.0437 ± 0.02 L d–1, respectively). Improvements to generalizability of modeling approaches will be reliant on the need for standardized guidelines for Rs measurement. The use of in silico tools for Rs determination represents a more economical approach than laboratory calibrations. PMID:27363449

A single-base deletion in the 3'-coding region of glycogen-debranching enzyme is prevalent in glycogen storage disease type IIIA in a population of North African Jewish patients.

PubMed

Parvari, R; Moses, S; Shen, J; Hershkovitz, E; Lerner, A; Chen, Y T

1997-01-01

Glycogen storage disease type III (GSD III) is an autosomal recessive disease caused by the deficiency of glycogen-debranching enzyme (AGL). The overall incidence of the disease is about 1:100,000 life births in the USA; however, it is unusually frequent among North African Jews in Israel (prevalence 1:5,400, carrier prevalence 1:35). All North African Jewish GSD III patients examined have both liver and muscle involvement. While all patients showed the characteristic features related to the liver enzyme deficiency, the peripheral muscular impairment varied from minimal to severe, with neuromuscular involvement. A single mutation in the AGL gene, the deletion of T at position 4,455 (4,455delT) in homozygous form, was found in this patient population. The mutation 4,455delT results in the change of 17 amino acids at the carboxy terminus of the AGL protein (1,486-1,502) and truncation of the last 30 amino acids of the normal AGL 1,532 amino acids. The mutation appears to be ethnic specific as it was not seen in 18 patients of different ethnic origins. This is the first report of a mutation in the AGL gene affecting a considerable number of GSD III patients in a defined population.

Efficacy of first-trimester ultrasound parameters for prediction of early spontaneous abortion.

PubMed

Datta, Mamta Rath; Raut, Ankush

2017-09-01

To assess first-trimester ultrasound measurements for the prediction of early spontaneous abortion. In a prospective observational study in Jamshedpur, India, women with singleton pregnancies of 42-76 days were enrolled between November 2014 and April 2016. Inclusion criteria were spontaneous conception, embryonic cardiac activity, and regular menstrual cycle. Fetal crown-to-rump length (CRL), gestational sac diameter (GSD), yolk sac diameter (YSD), and fetal heart rate (FHR) were measured by transvaginal ultrasonography. Ultrasonography was repeated at 12 weeks and beyond to determine pregnancy continuation. Among 800 women, 140 (17.5%) experienced early spontaneous abortion. CRL, GSD, and FHR values below the 5th percentile (odds ratio [OR] 26.48, 26.94, and 100.63, respectively), and YSD above the 95th percentile (OR 1.04) were predictors of early abortion. Normal YSD did not reduce the risk of abortion if the other three parameters were below the 5th percentile (OR 34.27). For every 10-bpm decrease in FHR below 130, there was 26.7% increased risk of abortion. GSD-CRL difference of less than 5 mm was associated with a higher likelihood of abortion (OR 4.88). First-trimester ultrasound measurements are predictors of early abortion. Risk assessment tables based on combinations of abnormal measures might improve prediction rates. © 2017 International Federation of Gynecology and Obstetrics.

In vitro digestibility of normal and waxy corn starch is modified by the addition of Tween 80.

PubMed

Vernon-Carter, E J; Alvarez-Ramirez, J; Bello-Perez, L A; Garcia-Hernandez, A; Roldan-Cruz, C; Garcia-Diaz, S

2018-05-15

Aqueous dispersions of normal and waxy corn starch (3% w/w) were mixed with Tween 80 (0, 7.5, 15, 22.5 and 30 g/100 g of starch), and gelatinized (90 °C, 20 min). Optical microscopy of the gelatinized starch dispersions (GSD x ; x = Tween 80 concentration) revealed that the microstructure was characterized by a continuous phase of leached amylose and amylopectin entangled chains, and a dispersed phase of insoluble remnants, called ghosts, on whose surface small granules were observed, imputed to Tween 80. The apparent viscosity of the GSD x decreased as the concentration of Tween 80 increased (up to about 70-90%). FTIR analysis of dried GSD x indicated that Tween 80 addition decreased short-range ordering. The content of rapidly digestible starch (RDS) and resistant starch (RS) fractions tended to increase significantly, at the expense of a significant decrease of slowly digestible starch (SDS) fraction, an effect that may be attributed to the increase of amorphous structures and starch chain-surfactant complexes. The RDS and RS increase was more pronounced for normal than for waxy corn starch, and the significance of the increase was dependent on Tween 80 concentration. Overall, the results showed that surfactant can affect largely the digestibility of starch chains. Copyright © 2018 Elsevier B.V. All rights reserved.

Retrospective dosimetry of Iodine-131 exposures using Iodine-129 and Caesium-137 inventories in soils--A critical evaluation of the consequences of the Chernobyl accident in parts of Northern Ukraine.

PubMed

Michel, R; Daraoui, A; Gorny, M; Jakob, D; Sachse, R; Romantschuk, L D; Alfimov, V; Synal, H-A

2015-12-01

The radiation exposure of thyroid glands due to (131)I as a consequence of the Chernobyl accident was investigated retrospectively based on (129)I and (137)Cs inventories in soils in Northern Ukraine. To this end, soil samples from 60 settlements were investigated for (129)I, (127)I, and (137)Cs by AMS, ICP-MS and gamma-spectrometry, respectively. Sampling was performed between 2004 und 2007. In those parts of Northern Ukraine investigated here the (129)I and (137)Cs inventories are well correlated, the variability of the individual (129)I/(137)Cs ratios being, however, high. Both the (129)I and (137)Cs inventories in the individual 5 samples for each settlement allowed estimating the uncertainties of the inventories due to the variability of the radionuclide deposition and consequently of the retrospective dosimetry. Thyroid equivalent doses were calculated from the (129)I and the (137)Cs inventories using aggregated dose coefficients for 5-year old and 10-year-old children as well as for adults. The highest thyroid equivalent doses (calculated from (129)I inventories) were calculated for Wladimirowka with 30 Gy for 5-years-old children and 7 Gy for adults. In 35 settlements of contamination zone II the geometric mean of the thyroid equivalent doses was 2.0 Gy for 5-years-old children with a geometric standard deviation (GSD) of 3.0. For adults the geometric mean was 0.47 Gy also with a GSD of 3.0. In more than 25 settlements of contamination zone III the geometric means were 0.82 Gy for 5-years old children with a GSD of 1.8 and 0.21 Gy for adults (GSD 1.8). For 45 settlements, the results of the retrospective dosimetry could be compared with thyroid equivalent doses calculated using time-integrated (131)I activities of thyroids which were measured in 1986. Thus, a critical evaluation of the results was possible which demonstrated the general feasibility of the method, but also the associated uncertainties and limitations. Copyright © 2015 Elsevier Ltd. All rights reserved.

Transcriptomic responses to environmental temperature by turtles with temperature-dependent and genotypic sex determination assessed by RNAseq inform the genetic architecture of embryonic gonadal development

PubMed Central

Radhakrishnan, Srihari; Literman, Robert; Neuwald, Jennifer; Severin, Andrew

2017-01-01

Vertebrate sexual fate is decided primarily by the individual’s genotype (GSD), by the environmental temperature during development (TSD), or both. Turtles exhibit TSD and GSD, making them ideal to study the evolution of sex determination. Here we analyze temperature-specific gonadal transcriptomes (RNA-sequencing validated by qPCR) of painted turtles (Chrysemys picta TSD) before and during the thermosensitive period, and at equivalent stages in soft-shell turtles (Apalone spinifera—GSD), to test whether TSD’s and GSD’s transcriptional circuitry is identical but deployed differently between mechanisms. Our data show that most elements of the mammalian urogenital network are active during turtle gonadogenesis, but their transcription is generally more thermoresponsive in TSD than GSD, and concordant with their sex-specific function in mammals [e.g., upregulation of Amh, Ar, Esr1, Fog2, Gata4, Igf1r, Insr, and Lhx9 at male-producing temperature, and of β-catenin, Foxl2, Aromatase (Cyp19a1), Fst, Nf-kb, Crabp2 at female-producing temperature in Chrysemys]. Notably, antagonistic elements in gonadogenesis (e.g., β-catenin and Insr) were thermosensitive only in TSD early-embryos. Cirbp showed warm-temperature upregulation in both turtles disputing its purported key TSD role. Genes that may convert thermal inputs into sex-specific development (e.g., signaling and hormonal pathways, RNA-binding and heat-shock) were differentially regulated. Jak-Stat, Nf-κB, retinoic-acid, Wnt, and Mapk-signaling (not Akt and Ras-signaling) potentially mediate TSD thermosensitivity. Numerous species-specific ncRNAs (including Xist) were differentially-expressed, mostly upregulated at colder temperatures, as were unannotated loci that constitute novel TSD candidates. Cirbp showed warm-temperature upregulation in both turtles. Consistent transcription between turtles and alligator revealed putatively-critical reptilian TSD elements for male (Sf1, Amh, Amhr2) and female (Crabp2 and Hspb1) gonadogenesis. In conclusion, while preliminary, our data helps illuminate the regulation and evolution of vertebrate sex determination, and contribute genomic resources to guide further research into this fundamental biological process. PMID:28296881

A pilot study to assess residential noise exposure near natural gas compressor stations.

PubMed

Boyle, Meleah D; Soneja, Sutyajeet; Quirós-Alcalá, Lesliam; Dalemarre, Laura; Sapkota, Amy R; Sangaramoorthy, Thurka; Wilson, Sacoby; Milton, Donald; Sapkota, Amir

2017-01-01

U.S. natural gas production increased 40% from 2000 to 2015. This growth is largely related to technological advances in horizontal drilling and high-volume hydraulic fracturing. Environmental exposures upon impacted communities are a significant public health concern. Noise associated with natural gas compressor stations has been identified as a major concern for nearby residents, though limited studies exist. We conducted a pilot study to characterize noise levels in 11 homes located in Doddridge County, West Virginia, and determined whether these levels differed based on time of day, indoors vs. outdoors, and proximity of homes to natural gas compressor stations. We also compared noise levels at increasing distances from compressor stations to available noise guidelines, and evaluated low frequency noise presence. We collected indoor and outdoor 24-hour measurements (Leq, 24hr) in eight homes located within 750 meters (m) of the nearest compressor station and three control homes located >1000m. We then evaluated how A-weighted decibel (dBA) exposure levels differed based on factors outlined above. The geometric mean (GM) for 24-hour outdoor noise levels at homes located <300m (Leq,24hr: 60.3 dBA; geometric standard deviation (GSD): 1.0) from the nearest compressor station was nearly 9 dBA higher than control homes (Leq,24hr: 51.6 dBA; GSD: 1.1). GM for 24 hour indoor noise for homes <300m (Leq,24hr: 53.4 dBA; GSD: 1.2) from the nearest compressor station was 11.2 dBA higher than control homes (Leq,24hr: 42.2 dBA; GSD: 1.1). Indoor average daytime noise for homes <300m of the nearest compressor stations were 13.1 dBA higher than control homes, while indoor nighttime readings were 9.4 dBA higher. Findings indicate that living near a natural gas compressor station could potentially result in high environmental noise exposures. Larger studies are needed to confirm these findings and evaluate potential health impacts and protection measures.

Influence of Gsd for 3d City Modeling and Visualization from Aerial Imagery

NASA Astrophysics Data System (ADS)

Alrajhi, Muhamad; Alam, Zafare; Afroz Khan, Mohammad; Alobeid, Abdalla

2016-06-01

Ministry of Municipal and Rural Affairs (MOMRA), aims to establish solid infrastructure required for 3D city modelling, for decision making to set a mark in urban development. MOMRA is responsible for the large scale mapping 1:1,000; 1:2,500; 1:10,000 and 1:20,000 scales for 10cm, 20cm and 40 GSD with Aerial Triangulation data. As 3D city models are increasingly used for the presentation exploration, and evaluation of urban and architectural designs. Visualization capabilities and animations support of upcoming 3D geo-information technologies empower architects, urban planners, and authorities to visualize and analyze urban and architectural designs in the context of the existing situation. To make use of this possibility, first of all 3D city model has to be created for which MOMRA uses the Aerial Triangulation data and aerial imagery. The main concise for 3D city modelling in the Kingdom of Saudi Arabia exists due to uneven surface and undulations. Thus real time 3D visualization and interactive exploration support planning processes by providing multiple stakeholders such as decision maker, architects, urban planners, authorities, citizens or investors with a three - dimensional model. Apart from advanced visualization, these 3D city models can be helpful for dealing with natural hazards and provide various possibilities to deal with exotic conditions by better and advanced viewing technological infrastructure. Riyadh on one side is 5700m above sea level and on the other hand Abha city is 2300m, this uneven terrain represents a drastic change of surface in the Kingdom, for which 3D city models provide valuable solutions with all possible opportunities. In this research paper: influence of different GSD (Ground Sample Distance) aerial imagery with Aerial Triangulation is used for 3D visualization in different region of the Kingdom, to check which scale is more sophisticated for obtaining better results and is cost manageable, with GSD (7.5cm, 10cm, 20cm and 40cm). The comparison test is carried out in Bentley environment to check the best possible results obtained through operating different batch processes.

A multicentre randomized controlled trial of an empowerment-inspired intervention for adolescents starting continuous subcutaneous insulin infusion--a study protocol.

PubMed

Brorsson, Anna Lena; Leksell, Janeth; Viklund, Gunnel; Lindholm Olinder, Anna

2013-12-20

Continuous subcutaneous insulin infusion (CSII) treatment among children with type 1 diabetes is increasing in Sweden. However, studies evaluating glycaemic control in children using CSII show inconsistent results. The distribution of responsibility for diabetes self-management between children and parents is often unclear and needs clarification. There is much published support for continued parental involvement and shared diabetes management during adolescence. Guided Self-Determination (GSD) is an empowerment-based, person-centred, reflection and problem solving method intended to guide the patient to become self-sufficient and develop life skills for managing difficulties in diabetes self-management. This method has been adapted for adolescents and parents as Guided Self-Determination-Young (GSD-Y). This study aims to evaluate the effect of an intervention with GSD-Y in groups of adolescents starting on insulin pumps and their parents on diabetes-related family conflicts, perceived health and quality of life (QoL), and metabolic control. Here, we describe the protocol and plans for study enrollment. This study is designed as a randomized, controlled, prospective, multicentre study. Eighty patients between 12-18 years of age who are planning to start CSII will be included. All adolescents and their parents will receive standard insulin pump training. The education intervention will be conducted when CSII is to be started and at four appointments in the first 4 months after starting CSII. The primary outcome is haemoglobin A1c levels. Secondary outcomes are perceived health and QoL, frequency of blood glucose self-monitoring and bolus doses, and usage of carbohydrate counting. The following instruments will be used: Disabkids, 'Check your health', the Diabetes Family Conflict Scale and the Swedish Diabetes Empowerment Scale. Outcomes will be evaluated within and between groups by comparing data at baseline, and at 6 and 12 months after starting treatment. In this study, we will assess the effect of starting an CSII together with the model of GSD to determine whether this approach leads to retention of improved glycaemic control, QoL, responsibility distribution and reduced diabetes-related conflicts in the family.

A pilot study to assess residential noise exposure near natural gas compressor stations

PubMed Central

Boyle, Meleah D.; Quirós-Alcalá, Lesliam; Dalemarre, Laura; Sapkota, Amy R.; Sangaramoorthy, Thurka; Wilson, Sacoby; Milton, Donald; Sapkota, Amir

2017-01-01

Background U.S. natural gas production increased 40% from 2000 to 2015. This growth is largely related to technological advances in horizontal drilling and high-volume hydraulic fracturing. Environmental exposures upon impacted communities are a significant public health concern. Noise associated with natural gas compressor stations has been identified as a major concern for nearby residents, though limited studies exist. Objectives We conducted a pilot study to characterize noise levels in 11 homes located in Doddridge County, West Virginia, and determined whether these levels differed based on time of day, indoors vs. outdoors, and proximity of homes to natural gas compressor stations. We also compared noise levels at increasing distances from compressor stations to available noise guidelines, and evaluated low frequency noise presence. Methods We collected indoor and outdoor 24-hour measurements (Leq, 24hr) in eight homes located within 750 meters (m) of the nearest compressor station and three control homes located >1000m. We then evaluated how A-weighted decibel (dBA) exposure levels differed based on factors outlined above. Results The geometric mean (GM) for 24-hour outdoor noise levels at homes located <300m (Leq,24hr: 60.3 dBA; geometric standard deviation (GSD): 1.0) from the nearest compressor station was nearly 9 dBA higher than control homes (Leq,24hr: 51.6 dBA; GSD: 1.1). GM for 24 hour indoor noise for homes <300m (Leq,24hr: 53.4 dBA; GSD: 1.2) from the nearest compressor station was 11.2 dBA higher than control homes (Leq,24hr: 42.2 dBA; GSD: 1.1). Indoor average daytime noise for homes <300m of the nearest compressor stations were 13.1 dBA higher than control homes, while indoor nighttime readings were 9.4 dBA higher. Conclusions Findings indicate that living near a natural gas compressor station could potentially result in high environmental noise exposures. Larger studies are needed to confirm these findings and evaluate potential health impacts and protection measures. PMID:28369113

HERCULES/MSI: a multispectral imager with geolocation for STS-70

NASA Astrophysics Data System (ADS)

Simi, Christopher G.; Kindsfather, Randy; Pickard, Henry; Howard, William, III; Norton, Mark C.; Dixon, Roberta

1995-11-01

A multispectral intensified CCD imager combined with a ring laser gyroscope based inertial measurement unit was flown on the Space Shuttle Discovery from July 13-22, 1995 (Space Transport System Flight No. 70, STS-70). The camera includes a six position filter wheel, a third generation image intensifier, and a CCD camera. The camera is integrated with a laser gyroscope system that determines the ground position of the imagery to an accuracy of better than three nautical miles. The camera has two modes of operation; a panchromatic mode for high-magnification imaging [ground sample distance (GSD) of 4 m], or a multispectral mode consisting of six different user-selectable spectral ranges at reduced magnification (12 m GSD). This paper discusses the system hardware and technical trade-offs involved with camera optimization, and presents imagery observed during the shuttle mission.

Comparing Sanitation Delivery Modalities in Urban Informal Settlement Schools: A Randomized Trial in Nairobi, Kenya

PubMed Central

Bohnert, Kate; Chard, Anna N.; Mwaki, Alex; Kirby, Amy E.; Muga, Richard; Nagel, Corey L.; Thomas, Evan A.; Freeman, Matthew C.

2016-01-01

The provision of safely managed sanitation in informal settlements is a challenge, especially in schools that require durable, clean, sex-segregated facilities for a large number of children. In informal settlements in Nairobi, school sanitation facilities demand considerable capital costs, yet are prone to breakage and often unhygienic. The private sector may be able to provide quality facilities and services to schools at lower costs as an alternative to the sanitation that is traditionally provided by the government. We conducted a randomized trial comparing private sector service delivery (PSSD) of urine-diverting dry latrines with routine waste collection and maintenance and government standard delivery (GSD) of cistern-flush toilets or ventilated improved pit latrines. The primary outcomes were facility maintenance, use, exposure to fecal contamination, and cost. Schools were followed for one school year. There were few differences in maintenance and pathogen exposure between PSSD and GSD toilets. Use of the PSSD toilets was 128% higher than GSD toilets, as measured with electronic motion detectors. The initial cost of private sector service delivery was USD 2053 (KES 210,000) per school, which was lower than the average cost of rehabilitating the government standard flush-type toilets (USD 9306 (KES 922,638)) and constructing new facilities (USD 114,889 (KES 1,169,668)). The private sector delivery of dry sanitation provided a feasible alternative to the delivery of sewage sanitation in Nairobi informal settlements and might elsewhere in sub-Saharan Africa. PMID:27916914

The upstream enhancer elements of the G6PC promoter are critical for optimal G6PC expression in murine glycogen storage disease type Ia.

PubMed

Lee, Young Mok; Pan, Chi-Jiunn; Koeberl, Dwight D; Mansfield, Brian C; Chou, Janice Y

2013-11-01

Glycogen storage disease type-Ia (GSD-Ia) patients deficient in glucose-6-phosphatase-α (G6Pase-α or G6PC) manifest impaired glucose homeostasis characterized by fasting hypoglycemia, growth retardation, hepatomegaly, nephromegaly, hyperlipidemia, hyperuricemia, and lactic acidemia. Two efficacious recombinant adeno-associated virus pseudotype 2/8 (rAAV8) vectors expressing human G6Pase-α have been independently developed. One is a single-stranded vector containing a 2864-bp of the G6PC promoter/enhancer (rAAV8-GPE) and the other is a double-stranded vector containing a shorter 382-bp minimal G6PC promoter/enhancer (rAAV8-miGPE). To identify the best construct, a direct comparison of the rAAV8-GPE and the rAAV8-miGPE vectors was initiated to determine the best vector to take forward into clinical trials. We show that the rAAV8-GPE vector directed significantly higher levels of hepatic G6Pase-α expression, achieved greater reduction in hepatic glycogen accumulation, and led to a better toleration of fasting in GSD-Ia mice than the rAAV8-miGPE vector. Our results indicated that additional control elements in the rAAV8-GPE vector outweigh the gains from the double-stranded rAAV8-miGPE transduction efficiency, and that the rAAV8-GPE vector is the current choice for clinical translation in human GSD-Ia. © 2013.

Gonad Transcriptome Analysis of High-Temperature-Treated Females and High-Temperature-Induced Sex-Reversed Neomales in Nile Tilapia

PubMed Central

Sun, Li Xue; Teng, Jian; Zhao, Yan; Li, Ning; Wang, Hui

2018-01-01

Background: Nowadays, the molecular mechanisms governing TSD (temperature-dependent sex determination) or GSD + TE (genotypic sex determination + temperature effects) remain a mystery in fish. Methods: We developed three all-female families of Nile tilapia (Oreochromis niloticus), and the family with the highest male ratio after high-temperature treatment was used for transcriptome analysis. Results: First, gonadal histology analysis indicated that the histological morphology of control females (CF) was not significantly different from that of high-temperature-treated females (TF) at various development stages. However, the high-temperature treatment caused a lag of spermatogenesis in high-temperature-induced neomales (IM). Next, we sequenced the transcriptome of CF, TF, and IM Nile tilapia. 79, 11,117, and 11,000 differentially expressed genes (DEGs) were detected in the CF–TF, CF–IM, and TF–IM comparisons, respectively, and 44 DEGs showed identical expression changes in the CF–TF and CF–IM comparisons. Principal component analysis (PCA) indicated that three individuals in CF and three individuals in TF formed a cluster, and three individuals in IM formed a distinct cluster, which confirmed that the gonad transcriptome profile of TF was similar to that of CF and different from that of IM. Finally, six sex-related genes were validated by qRT-PCR. Conclusions: This study identifies a number of genes that may be involved in GSD + TE, which will be useful for investigating the molecular mechanisms of TSD or GSD + TE in fish. PMID:29495590

Gonad Transcriptome Analysis of High-Temperature-Treated Females and High-Temperature-Induced Sex-Reversed Neomales in Nile Tilapia.

PubMed

Sun, Li Xue; Teng, Jian; Zhao, Yan; Li, Ning; Wang, Hui; Ji, Xiang Shan

2018-02-28

Nowadays, the molecular mechanisms governing TSD (temperature-dependent sex determination) or GSD + TE (genotypic sex determination + temperature effects) remain a mystery in fish. We developed three all-female families of Nile tilapia ( Oreochromis niloticus ), and the family with the highest male ratio after high-temperature treatment was used for transcriptome analysis. First, gonadal histology analysis indicated that the histological morphology of control females (CF) was not significantly different from that of high-temperature-treated females (TF) at various development stages. However, the high-temperature treatment caused a lag of spermatogenesis in high-temperature-induced neomales (IM). Next, we sequenced the transcriptome of CF, TF, and IM Nile tilapia. 79, 11,117, and 11,000 differentially expressed genes (DEGs) were detected in the CF-TF, CF-IM, and TF-IM comparisons, respectively, and 44 DEGs showed identical expression changes in the CF-TF and CF-IM comparisons. Principal component analysis (PCA) indicated that three individuals in CF and three individuals in TF formed a cluster, and three individuals in IM formed a distinct cluster, which confirmed that the gonad transcriptome profile of TF was similar to that of CF and different from that of IM. Finally, six sex-related genes were validated by qRT-PCR. This study identifies a number of genes that may be involved in GSD + TE, which will be useful for investigating the molecular mechanisms of TSD or GSD + TE in fish.

Epigenetic modification and inheritance in sexual reversal of fish.

PubMed

Shao, Changwei; Li, Qiye; Chen, Songlin; Zhang, Pei; Lian, Jinmin; Hu, Qiaomu; Sun, Bing; Jin, Lijun; Liu, Shanshan; Wang, Zongji; Zhao, Hongmei; Jin, Zonghui; Liang, Zhuo; Li, Yangzhen; Zheng, Qiumei; Zhang, Yong; Wang, Jun; Zhang, Guojie

2014-04-01

Environmental sex determination (ESD) occurs in divergent, phylogenetically unrelated taxa, and in some species, co-occurs with genetic sex determination (GSD) mechanisms. Although epigenetic regulation in response to environmental effects has long been proposed to be associated with ESD, a systemic analysis on epigenetic regulation of ESD is still lacking. Using half-smooth tongue sole (Cynoglossus semilaevis) as a model-a marine fish that has both ZW chromosomal GSD and temperature-dependent ESD-we investigated the role of DNA methylation in transition from GSD to ESD. Comparative analysis of the gonadal DNA methylomes of pseudomale, female, and normal male fish revealed that genes in the sex determination pathways are the major targets of substantial methylation modification during sexual reversal. Methylation modification in pseudomales is globally inherited in their ZW offspring, which can naturally develop into pseudomales without temperature incubation. Transcriptome analysis revealed that dosage compensation occurs in a restricted, methylated cytosine enriched Z chromosomal region in pseudomale testes, achieving equal expression level in normal male testes. In contrast, female-specific W chromosomal genes are suppressed in pseudomales by methylation regulation. We conclude that epigenetic regulation plays multiple crucial roles in sexual reversal of tongue sole fish. We also offer the first clues on the mechanisms behind gene dosage balancing in an organism that undergoes sexual reversal. Finally, we suggest a causal link between the bias sex chromosome assortment in the offspring of a pseudomale family and the transgenerational epigenetic inheritance of sexual reversal in tongue sole fish.

Emergency visits and occupational hazards in German Shepherd police dogs (2008-2010).

PubMed

Parr, Joanna R; Otto, Cynthia M

2013-01-01

To describe the most common reasons for emergency medical visits in working police dogs. Retrospective case control study. Two hundred three German Shepherd dogs (GSDs); 138 police dog visits by 74 dogs and 138 pet (control) dog visits by 129 dogs. Medical records of all GSDs seen in the emergency service (ES) at the University of Pennsylvania Veterinary Hospital from July 2008 to July 2010 were reviewed. The recorded diagnoses from police GSD ES visits (defined as a new problem or new episode of a recurrent problem) were compared to those of randomly chosen pet GSD ES visits. There were significantly more intact male police GSDs than pet GSDs. Police GSDs were significantly younger than pet GSDs. The most common presenting complaint in both groups was gastrointestinal disease (eg, vomiting, diarrhea, gastric dilatation and volvulus). Pet GSDs were significantly more likely to present for geriatric conditions (eg, central nervous system disease, cardiovascular disease, and neoplastic conditions). Orthopedic issues were significantly more common in police GSDs. Police GSDs are more likely to present for orthopedic injuries and less likely to present for geriatric diseases. Gastrointestinal disease is not unique to the working GSD and was equally represented in both populations. Preventative measures for all GSDs should focus on minimizing gastrointestinal disease. Preventive strategies focusing on physical fitness and conditioning as well as selective breeding programs may help reduce orthopedic injuries in police GSDs. © Veterinary Emergency and Critical Care Society 2013.

Comparing Sanitation Delivery Modalities in Urban Informal Settlement Schools: A Randomized Trial in Nairobi, Kenya.

PubMed

Bohnert, Kate; Chard, Anna N; Mwaki, Alex; Kirby, Amy E; Muga, Richard; Nagel, Corey L; Thomas, Evan A; Freeman, Matthew C

2016-11-30

The provision of safely managed sanitation in informal settlements is a challenge, especially in schools that require durable, clean, sex-segregated facilities for a large number of children. In informal settlements in Nairobi, school sanitation facilities demand considerable capital costs, yet are prone to breakage and often unhygienic. The private sector may be able to provide quality facilities and services to schools at lower costs as an alternative to the sanitation that is traditionally provided by the government. We conducted a randomized trial comparing private sector service delivery (PSSD) of urine-diverting dry latrines with routine waste collection and maintenance and government standard delivery (GSD) of cistern-flush toilets or ventilated improved pit latrines. The primary outcomes were facility maintenance, use, exposure to fecal contamination, and cost. Schools were followed for one school year. There were few differences in maintenance and pathogen exposure between PSSD and GSD toilets. Use of the PSSD toilets was 128% higher than GSD toilets, as measured with electronic motion detectors. The initial cost of private sector service delivery was USD 2053 (KES 210,000) per school, which was lower than the average cost of rehabilitating the government standard flush-type toilets (USD 9306 (KES 922,638)) and constructing new facilities (USD 114,889 (KES 1,169,668)). The private sector delivery of dry sanitation provided a feasible alternative to the delivery of sewage sanitation in Nairobi informal settlements and might elsewhere in sub-Saharan Africa.

Review and retrospective analysis of degenerative lumbosacral stenosis in 156 dogs treated by dorsal laminectomy.

PubMed

Suwankong, N; Meij, B P; Voorhout, G; de Boer, A H; Hazewinkel, H A W

2008-01-01

The medical records of 156 dogs with degenerative lumbosacral stenosis (DLS) that underwent decompressive surgery were reviewed for signalment, history, clinical signs, imaging and surgical findings. The German Shepherd Dog (GSD) was most commonly affected (40/156, 25.6%). Pelvic limb lameness, caudal lumbar pain and pain evoked by lumbosacral pressure were the most frequent clinical findings. Radiography showed lumbosacral step formation in 78.8% (93/118) of the dogs which was associated with elongation of the sacral lamina in 18.6% (22/118). Compression of the cauda equina was diagnosed by imaging (epidurography, CT, or MRI) in 94.2% (147/156) of the dogs. Loss of the bright nucleus pulposus signal of the L7-S1 disc was found on T2-weighted MR images in 73.5% (25/34) of the dogs. The facet joint angle at L7-S1 was significantly smaller, and the tropism greater in GSD than in the other dog breeds. The smaller facet joint angle and higher incidence of tropism seen in the GSD may predispose this breed to DLS. Epidurography, CT, and MRI allow adequate visualization of cauda equina compression. During surgery, disc protrusion was found in 70.5% (110/156) of the dogs. Overall improvement after surgery was recorded in the medical records in 79.0% (83/105) of the dogs. Of the 38 owners that responded to questionnaires up to five years after surgery, 29 (76%) perceived an improvement.

Glycogen Synthesis in Glycogenin 1-Deficient Patients: A Role for Glycogenin 2 in Muscle.

PubMed

Krag, Thomas O; Ruiz-Ruiz, Cristina; Vissing, John

2017-08-01

Glycogen storage disease (GSD) type XV is a rare disease caused by mutations in the GYG1 gene that codes for the core molecule of muscle glycogen, glycogenin 1. Nonetheless, glycogen is present in muscles of glycogenin 1-deficient patients, suggesting an alternative for glycogen buildup. A likely candidate is glycogenin 2, an isoform expressed in the liver and heart but not in healthy skeletal muscle. We wanted to investigate the formation of glycogen and changes in glycogen metabolism in patients with GSD type XV. Two patients with mutations in the GYG1 gene were investigated for histopathology, ultrastructure, and expression of proteins involved in glycogen synthesis and metabolism. Apart from occurrence of polyglucosan (PG) bodies in few fibers, glycogen appeared normal in most cells, and the concentration was normal in patients with GSD type XV. We found that glycogenin 1 was absent, but glycogenin 2 was present in the patients, whereas the opposite was the case in healthy controls. Electron microscopy revealed that glycogen was present between and not inside myofibrils in type II fibers, compromising the ultrastructure of these fibers, and only type I fibers contained PG bodies. We also found significant changes to the expression levels of several enzymes directly involved in glycogen and glucose metabolism. To our knowledge, this is the first report demonstrating expression of glycogenin 2 in glycogenin 1-deficient patients, suggesting that glycogenin 2 rescues the formation of glycogen in patients with glycogenin 1 deficiency. Copyright © 2017 Endocrine Society

Genetics Home Reference: glycogen storage disease type IX

MedlinePlus

... Health Conditions Glycogen storage disease type IX Glycogen storage disease type IX Printable PDF Open All Close ... to view the expand/collapse boxes. Description Glycogen storage disease type IX (also known as GSD IX) ...

Type V glycogen storage disease

MedlinePlus

Type V glycogen storage disease (GSD V) is a rare inherited condition in which the body is not able to break down glycogen. ... provide more information and resources: Association for Glycogen Storage Disease -- www.agsdus.org National Organization for Rare ...

Electrochemical characterization of gelatinized starch dispersions: voltammetry and electrochemical impedance spectroscopy on platinum surface.

PubMed

Hernandez-Jaimes, C; Lobato-Calleros, C; Sosa, E; Bello-Pérez, L A; Vernon-Carter, E J; Alvarez-Ramirez, J

2015-06-25

The electrochemical properties of gelatinized starch dispersions (GSD; 5% w/w) from different botanical sources were studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) tests over a platinum surface. The phenomenological modelling of EIS data using equivalent circuits indicated that after gelatinization the electrical resistance was determined mainly by the resistance of insoluble material (i.e., ghosts). Sonication of the GSD disrupted the ghost microstructure, and produced an increase in electrical conductivity by reducing the resistance of the insoluble material. The CV data showed three oxidation peaks at potentials where glucose solutions displayed oxidation waves. It is postulated that hydrolysis at the bulk and electrocatalyzed oxidation on the Pt-surface are reactions involved in the starch transformation. Starches peak intensity increased with the amylose content, suggesting that the amylose-rich matrix played an important role in the charge transfer in the electrolytic system. Copyright © 2015 Elsevier Ltd. All rights reserved.

Evaluation of the rostral projection of the sacral lamina as a component of degenerative lumbosacral stenosis in German shepherd dogs.

PubMed

Saunders, Harvey; Worth, Andrew J; Bridges, Janis P; Hartman, Angela

2018-05-20

To determine the association between a greater rostral projection of the sacral lamina and clinical signs of cauda equina syndrome (CES) in German shepherd dogs (GSD) with presumptive degenerative lumbosacral disease (DLSS). Retrospective cohort study. One hundred forty-three GSD (125 police dogs and 18 pet dogs) presenting for either CES or prebreeding evaluation. Fifty-five were classified as affected by CES and diagnosed with DLSS, and 88 were classified as unaffected on the basis of clinical and imaging findings. The position of the rostral edge of the sacral lamina was measured from radiographs and/or computed tomography (CT) scans. This position was compared between affected and unaffected dogs. In dogs that underwent both radiography and CT scanning, the agreement between sacral lamina localization using each imaging modality was determined. Owners/handlers were contacted to determine whether dogs subsequently developed clinical signs compatible with CES at a mean of 29 months (unaffected). The sacral lamina did not extend as far rostrally in affected dogs, compared to unaffected dogs (P = .04). Among the 88 dogs unaffected by CES at initial evaluation, 2 developed clinical signs consistent with CES at follow-up. Rostral projection of the sacral lamina, previously proposed as a potential risk factor in dogs with CES due to lumbosacral degeneration, was not associated with a diagnosis of DLSS in this study; the opposite was true. Rostral projection of the sacral lamina may not be a predisposing factor in the development of CES due to DLSS in GSD. © 2018 The American College of Veterinary Surgeons.

Karyotype analysis and sex determination in Australian Brush-turkeys (Alectura lathami).

PubMed

Ortega, Madison T; Foote, Dustin J; Nees, Nicholas; Erdmann, Jason C; Bangs, Charles D; Rosenfeld, Cheryl S

2017-01-01

Sexual differentiation across taxa may be due to genetic sex determination (GSD) and/or temperature sex determination (TSD). In many mammals, males are heterogametic (XY); whereas females are homogametic (XX). In most birds, the opposite is the case with females being heterogametic (ZW) and males the homogametic sex (ZZ). Many reptile species lack sex chromosomes, and instead, sexual differentiation is influenced by temperature with specific temperatures promoting males or females varying across species possessing this form of sexual differentiation, although TSD has recently been shown to override GSD in Australian central beaded dragons (Pogona vitticeps). There has been speculation that Australian Brush-turkeys (Alectura lathami) exhibit TSD alone and/or in combination with GSD. Thus, we sought to determine if this species possesses sex chromosomes. Blood was collected from one sexually mature female and two sexually mature males residing at Sylvan Heights Bird Park (SHBP) and shipped for karyotype analysis. Karyotype analysis revealed that contrary to speculation, Australian Brush-turkeys possess the classic avian ZW/ZZ sex chromosomes. It remains a possibility that a biased primary sex ratio of Australian Brush-turkeys might be influenced by maternal condition prior to ovulation that result in her laying predominantly Z- or W-bearing eggs and/or sex-biased mortality due to higher sensitivity of one sex in environmental conditions. A better understanding of how maternal and extrinsic factors might differentially modulate ovulation of Z- or W-bearing eggs and hatching of developing chicks possessing ZW or ZZ sex chromosomes could be essential in conservation strategies used to save endangered members of Megapodiidae.

Karyotype analysis and sex determination in Australian Brush-turkeys (Alectura lathami)

PubMed Central

Ortega, Madison T.; Foote, Dustin J.; Nees, Nicholas; Erdmann, Jason C.; Bangs, Charles D.

2017-01-01

Sexual differentiation across taxa may be due to genetic sex determination (GSD) and/or temperature sex determination (TSD). In many mammals, males are heterogametic (XY); whereas females are homogametic (XX). In most birds, the opposite is the case with females being heterogametic (ZW) and males the homogametic sex (ZZ). Many reptile species lack sex chromosomes, and instead, sexual differentiation is influenced by temperature with specific temperatures promoting males or females varying across species possessing this form of sexual differentiation, although TSD has recently been shown to override GSD in Australian central beaded dragons (Pogona vitticeps). There has been speculation that Australian Brush-turkeys (Alectura lathami) exhibit TSD alone and/or in combination with GSD. Thus, we sought to determine if this species possesses sex chromosomes. Blood was collected from one sexually mature female and two sexually mature males residing at Sylvan Heights Bird Park (SHBP) and shipped for karyotype analysis. Karyotype analysis revealed that contrary to speculation, Australian Brush-turkeys possess the classic avian ZW/ZZ sex chromosomes. It remains a possibility that a biased primary sex ratio of Australian Brush-turkeys might be influenced by maternal condition prior to ovulation that result in her laying predominantly Z- or W-bearing eggs and/or sex-biased mortality due to higher sensitivity of one sex in environmental conditions. A better understanding of how maternal and extrinsic factors might differentially modulate ovulation of Z- or W-bearing eggs and hatching of developing chicks possessing ZW or ZZ sex chromosomes could be essential in conservation strategies used to save endangered members of Megapodiidae. PMID:28910392

Urinary heavy metal levels and relevant factors among people exposed to e-waste dismantling.

PubMed

Wang, Hongmei; Han, Mei; Yang, Suwen; Chen, Yanqing; Liu, Qian; Ke, Shen

2011-01-01

Primitive electronic waste (e-waste) recycling has become a growing environmental concern, and toxic heavy metals released from e-waste activities may continue to threaten the health of local people. To study the impact of heavy metals in people around e-waste sites, 349 people from e-waste recycling sites (exposure group) and 118 people from a green plantation (control group) were surveyed, and their urinary levels of lead (UPb), cadmium (UCd), manganese (UMn), copper (UCu), and Zinc (UZn) were assayed. Questionnaire surveys for risk factors were also performed and analyzed by using the Pearson correlation analysis. Results indicated that the levels of urinary Cd in both occupational dismantling people {GM(GSD) 0.72(0.71) ug/L} and non-occupational dismantling people {GM(GSD) 0.50(0.79) ug/L} were higher than the control group {GM(GSD) 0.27(0.85) ug/L}. Further analyses of correlations between urinary heavy metal levels and exposure factors in the exposed group revealed positive relationship between the duration of dismantling and the level of UPb (p < 0.05). Meanwhile, rice sources from local village have a positive correlation with the level of UPb and UCd (p < 0.01). Other factors, however, may also have influences on heavy metal burden, and not all urinary heavy metal levels can be contributed to e-waste dismantling exposure levels. Primitive e-waste recycling activities may contribute to the changes of urinary heavy metal levels and increase the health risk for those chronically working on e-waste dismantling. Copyright © 2010 Elsevier Ltd. All rights reserved.

Using Grain-Size Distribution Methods for Estimation of Air Permeability.

PubMed

Wang, Tiejun; Huang, Yuanyang; Chen, Xunhong; Chen, Xi

2016-01-01

Knowledge of air permeability (ka ) at dry conditions is critical for the use of air flow models in porous media; however, it is usually difficult and time consuming to measure ka at dry conditions. It is thus desirable to estimate ka at dry conditions from other readily obtainable properties. In this study, the feasibility of using information derived from grain-size distributions (GSDs) for estimating ka at dry conditions was examined. Fourteen GSD-based equations originally developed for estimating saturated hydraulic conductivity were tested using ka measured at dry conditions in both undisturbed and disturbed river sediment samples. On average, the estimated ka from all the equations, except for the method of Slichter, differed by less than ± 4 times from the measured ka for both undisturbed and disturbed groups. In particular, for the two sediment groups, the results given by the methods of Terzaghi and Hazen-modified were comparable to the measured ka . In addition, two methods (e.g., Barr and Beyer) for the undisturbed samples and one method (e.g., Hazen-original) for the undisturbed samples were also able to produce comparable ka estimates. Moreover, after adjusting the values of the coefficient C in the GSD-based equations, the estimation of ka was significantly improved with the differences between the measured and estimated ka less than ±4% on average (except for the method of Barr). As demonstrated by this study, GSD-based equations may provide a promising and efficient way to estimate ka at dry conditions. © 2015, National Ground Water Association.

Comet C/2012 S1 (ISON)'s carbon-rich and micron-size-dominated coma dust

NASA Astrophysics Data System (ADS)

Wooden, D.; De Buizer, J.; Kelley, M.; Sitko, M.; Woodward, C.; Harker, D.; Reach, W.; Russell, R.; Kim, D.; Yanamadra-Fisher, P.; Lisse, C.; de Pater, I.; Gehrz, R.; Kolokolova, L.

2014-07-01

Comet C/2012 S1 (ISON) was unique in that it was a dynamically new comet derived from the Nearly Isotropic Oort cloud reservoir of comets with a sun-grazing orbit. We present thermal models for comet ISON (r_h ˜ 1.15 au, 2013-Oct-25 11:30 UT) that reveal comet ISON's dust was carbon-rich and dominated by a steep (and therefor narrow) grain size distribution (GSD) dominated by ˜ micron-sized grains. We constrained the models by our SOFIA FORCAST photometry at 11.1, 19.7 and 31.5 μ m and by a silicate feature strength of ˜1.1 and an 8-13 μ m continuum greybody color temperature of ˜275-280 K (using T_{bb}∝ {r}_h^{-0.5} and T_{bb}˜260-265 K from Subaru+COMICS, 2013-Oct-19 UT) [1,2]. Spectra of comet ISON with IRTF+BASS (2013-Nov-11-12 UT) also show a silicate feature strength of ˜1.1 as well as an 11.2 μ m forsterite peak [3]. Our thermal models [6], which employ 0.1-1000 μ m grains, yield constraints for the dust composition as well as GSD parameters of slope, peak grain size, porosity: ISON's dust has a low silicate-to-amorphous carbon ratio (˜1:9), the GSD has a steep slope (N≃4.5), a peak grain radius of ˜0.7 μ m, and moderately porous grains. Specifically, the 8-13 μ m continuum color temperature implies submicron- to micron-size grains and the steep fall off of the SOFIA far-IR photometry requires the GSD to have fewer relative numbers of larger and cooler grains compared to smaller and hotter grains. A IR proxy for the dust production rate is ɛ f ρ ˜ 1500 cm [4], which is akin to but larger than Afρ in scattered light (2013-Oct-20 UT, Afρ=796 cm(±5 %) in V-band from Swift) [5]. Also, ISON had a moderate-to-low dust-to-gas ratio [6]. Comet ISON's dust composition and GSD properties are distinct from the few well-studied long-period Nearly Isotropic Comets (NICs) that all had 'typical' GSD slopes (3.4≤N≤3.7) and silicate-to-amorphous carbon ratios ≫1 as well as the following properties: C/1995 O1 (Hale-Bopp)[7,8,9,10] and C/2001 Q4 (NEAT)[11] had smaller and highly porous grains, whereas C/2007 N4 (Lulin)[12] and C/2006 P1 (McNaught)[13] had larger and compact porous grains. Radial transport to comet-forming disk distances (≥ 20 au) is easier for smaller grains than for larger grains (≤ 1 μ m vs.˜20 μ m-like Stardust terminal particles) [14]. Perhaps Comet ISON formed either earlier in disk evolution whereby larger grains did not have the time to be transported to distances beyond Neptune, or the comet formed so far out in the disk that larger grains did not traverse such large radial distances. The high carbon-content of ISON's refractory dust appears to be complimented by the presence of limited-lifetime organic (CHON-like) grain materials: preliminary analyses of near-IR and high-resolution optical spectra indicate that gas-phase daughter molecules C_2, CN, and CH were more abundant than their parent molecules (HCN, C_2H_2, C_2H_6, measured in the near-IR) [15]. Dust composition as well as grain size distribution parameters (slope, peak grain size, and porosity) give clues to comet origins [16,17].

Genetics Home Reference: glycogen storage disease type 0

MedlinePlus

... complex sugar called glycogen , which is a major source of stored energy in the body. GSD 0 has two types: ... these cells do not have glycogen as a source of stored energy to draw upon following physical activity or fasting. ...

River network bedload model: a tool to investigate the impact of flow regulation on grain size distribution in a large Alpine catchment

NASA Astrophysics Data System (ADS)

Costa, Anna; Molnar, Peter

2017-04-01

Sediment transport rates along rivers and the grain size distribution (GSD) of coarse channel bed sediment are the result of the long term balance between transport capacity and sediment supply. Transport capacity, mainly a function of channel geometry and flow competence, can be altered by changes in climatic forcing as well as by human activities. In Alpine rivers it is hydropower production systems that are the main causes of modification to the transport capacity of water courses through flow regulation, leading over longer time scales to the adjustment of river bed GSDs. We developed a river network bedload transport model to evaluate the impacts of hydropower on the transfer of sediments and the GSDs of the Upper Rhône basin, a 5,200 km2 catchment located in the Swiss Alps. Many large reservoirs for hydropower production have been built along the main tributaries of the Rhône River since the 1960s, resulting in a complex system of intakes, tunnels, and pumping stations. Sediment storage behind dams and intakes, is accompanied by altered discharge due to hydropower operations, mainly higher flow in winter and lower in summer. It is expected that this change in flow regime may have resulted in different bedload transport. However, due the non-linear, threshold-based nature of the relation between discharge and sediment mobilization, the effects of changed hydraulic conditions are not easily deducible, and because observations of bedload in pre- and post-dam conditions are usually not available, a modelling approach is often necessary. In our modelling approach, the river network is conceptualized as a series of connected links (river reaches). Average geometric characteristics of each link (width, length, and slope of cross section) are extracted from digital elevation data, while surface roughness coefficients are assigned based on the GSD. Under the assumptions of rectangular prismatic cross sections and normal flow conditions, bed shear stress is estimated from available time series of daily discharge distributed along the river network. Potential bedload transport is estimated by the Wilcock and Crowe surface-based model for the entire GSD. Mass balance between transport capacity and sediment supply, applied to each individual grain size, determines the actual transport and the resulting GSD of the channel bed. Channel bed erosion is allowed through a long-term erosion rate. Sediment input from hillslopes is included as lateral sediment flux. Initial and boundary conditions are set based on available data of GSDs, while an approximation of the depth of the mobile bed is selected through sensitivity analysis. With the river network bedload model we aim to estimate the effect of flow regulation, i.e. altered transport capacity, on sediment transport and GSD of the entire Rhône river system. The model can also be applied as a tool to explore possible changes in bedload transport and channel GSDs under different discharge scenarios based, for example, on climate change projections or modified hydropower operation policies.

Grain size distribution of fault rocks: implication from natural gouges and high velocity friction experiments

NASA Astrophysics Data System (ADS)

Yang, X.; Chen, J.; Duan, B.

2011-12-01

The grain size distribution (GSD) is considered as an important parameter for the characterization of fault rocks. The relative magnitude of energy radiated as seismic waves to fracture energy plays a fundamental role to influence earthquake rupture dynamics. Currently, the details of grain size reduction mechanism and energy-budget are not well known. Here we present GSD measurements on fault rocks (gouge and breccias) in the main slip zone associated with the Wenchuan earthquake happened on 12 May, 2008, and on the gouges produced by high velocity friction (HVF) experiments. High velocity friction experiments were carried out on air dry granitic powder with grain size of 150 - 300 μm at normal stress of 1.0 MPa, a slip rate of 1.0 m / s and slip distances from 10 m to 30 m. On log-log plots of N(r) versus equivalent radius, two distinct linear parts can be discriminated with their intersection at 1 - 2 μm, defined as critical radius rc. One of power-law regime spans about 4 decades from 4 μm to 16 mm and the other covers a range of 0.2 - 2.0 μm. Larger fractal dimension from 2.7 to 3.5 are obtained for larger grain size regime, while lower values ranging from 1.7 to 2.1 for smaller size one. This two-stage distribution means the GSD is not self-similar (scale invariant) and the dominant ways of reducing grain size may be different from one another. XRD data show that the content of quartz drops greatly or disappears at 0.5 - 0.25 μm. GSD of HVF experimental products demonstrates similar feature to natural gouges. For instance, they all show the two-stage GSD with 1 - 2 μm of critical radius rc. The grains with their sizes of less than 1 μm appear rounded edges and equiaxial shapes. A variation in grain shapes can be observed in the grains larger than 5 μm. Some implications could be obtained from the measurements and experiments. (1) rc corresponds to the average value of grinding limit of rock-forming minerals. Further grain size reducing could be attributed to attrition during post-rupture processing such as steady-slip. (2) 90 % minerals with their sizes smaller than 0.5 μm is clays whose origin is neither associated with initially rupturing nor further grain attrition if we consider clay minerals within gouges as the products associated with fluid processes in inter-seismic intervals rather than by seismic slipping. (3) It is the grain that is created by the rupture process during earthquake could be used to calculate fracture energy. On the other hand, the grains forming in attrition during fault slip or / and inter-seismic intervals need to be picked out in order to get reasonable result. As example, if using D = 3.5 over the entire grain size range, the surface fracture energy will be over-estimated more than one order. Hence, surface fracture energy is a very small fraction in the total energy-budget of the earthquake.

Calculation of in situ acoustic sediment attenuation using off-the-shelf horizontal ADCPs in low concentration settings

USGS Publications Warehouse

Haught, Dan; Venditti, Jeremy G.; Wright, Scott A.

2017-01-01

The use of “off-the-shelf” acoustic Doppler velocity profilers (ADCPs) to estimate suspended sediment concentration and grain-size in rivers requires robust methods to estimate sound attenuation by suspended sediment. Theoretical estimates of sediment attenuation require a priori knowledge of the concentration and grain-size distribution (GSD), making the method impractical to apply in routine monitoring programs. In situ methods use acoustic backscatter profile slope to estimate sediment attenuation, and are a more attractive option. However, the performance of in situ sediment attenuation methods has not been extensively compared to theoretical methods. We used three collocated horizontally mounted ADCPs in the Fraser River at Mission, British Columbia and 298 observations of concentration and GSD along the acoustic beams to calculate theoretical and in situ sediment attenuation. Conversion of acoustic intensity from counts to decibels is influenced by the instrument noise floor, which affects the backscatter profile shape and therefore in situ attenuation. We develop a method that converts counts to decibels to maximize profile length, which is useful in rivers where cross-channel acoustic profile penetration is a fraction of total channel width. Nevertheless, the agreement between theoretical and in situ attenuation is poor at low concentrations because cross-stream gradients in concentration, sediment size or GSD can develop, which affect the backscatter profiles. We establish threshold concentrations below which in situ attenuation is unreliable in Fraser River. Our results call for careful examination of cross-stream changes in suspended sediment characteristics and acoustic profiles across a range of flows before in situ attenuation methods are applied in river monitoring programs.

Exposure to 4,4'-methylene bis (2-chloroaniline) (MbOCA) in New South Wales, Australia

PubMed Central

Shankar, Kiran; Fung, Vivian; Seneviratne, Mahinda; O'Donnell, Gregory E

2017-01-01

Objectives: This study was conducted to determine the level of exposure of 4,4'-methylene bis (2-chloroaniline) (MbOCA) in New South Wales (NSW), Australia. Methods: An integrated occupational hygiene and biological monitoring program were used to assess the workers' exposure to MbOCA via inhalation, ingestion and dermal contact. This was conducted by personal air monitoring, static air monitoring and surface contamination monitoring of the work environment and biological monitoring of the workers' exposure to MbOCA at nine workplaces in NSW. Results: The air monitoring results for MbOCA gave a geometric mean (GM) of 0.06 μg/m3 and a geometric standard deviation (GSD) of 2.70 and a 95% confidence interval of 0.29 μg/m3. The surface contamination in the main work area showed the highest contamination with a GM of 74 ng/cm2 and a GSD of 17 and a 95% confidence interval of 7,751 ng/cm2. Biological monitoring showed a GM of 0.89 μmol/mol cr and a GSD of 11.9 and a 95% confidence interval of 52 μmol/mol cr. This indicated that 13% of the workers were over the SafeWork NSW Biological Occupational Exposure Limit of 15 μmol/mol cr. Conclusions: Workers' exposure through inhalation was minimal; however, evidence from biological monitoring of MbOCA suggested that the main contributing factor to exposure was skin absorption. This was attributed to poor housekeeping and inadequate personal protection. Improvements in these areas were recommended, and it was also recommended to improve the awareness of the workers to the adverse effects to their health of exposure to this carcinogen. PMID:28320979

The evolution of environmental and genetic sex determination in fluctuating environments.

PubMed

Van Dooren, Tom J M; Leimar, Olof

2003-12-01

Twenty years ago, Bulmer and Bull suggested that disruptive selection, produced by environmental fluctuations, can result in an evolutionary transition from environmental sex determination (ESD) to genetic sex determination (GSD). We investigated the feasibility of such a process, using mutation-limited adaptive dynamics and individual-based computer simulations. Our model describes the evolution of a reaction norm for sex determination in a metapopulation setting with partial migration and variation in an environmental variable both within and between local patches. The reaction norm represents the probability of becoming a female as a function of environmental state and was modeled as a sigmoid function with two parameters, one giving the location (i.e., the value of the environmental variable for which an individual has equal chance of becoming either sex) and the other giving the slope of the reaction norm for that environment. The slope can be interpreted as being set by the level of developmental noise in morph determination, with less noise giving a steeper slope and a more switchlike reaction norm. We found convergence stable reaction norms with intermediate to large amounts of developmental noise for conditions characterized by low migration rates, small differential competitive advantages between the sexes over environments, and little variation between individual environments within patches compared to variation between patches. We also considered reaction norms with the slope parameter constrained to a high value, corresponding to little developmental noise. For these we found evolutionary branching in the location parameter and a transition from ESD toward GSD, analogous to the original analysis by Bulmer and Bull. Further evolutionary change, including dominance evolution, produced a polymorphism acting as a GSD system with heterogamety. Our results point to the role of developmental noise in the evolution of sex determination.

Relationship between environmental phthalate exposure and the intelligence of school-age children.

PubMed

Cho, Soo-Churl; Bhang, Soo-Young; Hong, Yun-Chul; Shin, Min-Sup; Kim, Boong-Nyun; Kim, Jae-Won; Yoo, Hee-Jung; Cho, In Hee; Kim, Hyo-Won

2010-07-01

Concern over phthalates has emerged because of their potential toxicity to humans. We investigated the relationship between the urinary concentrations of phthalate metabolites and children's intellectual functioning. This study enrolled 667 children at nine elementary schools in five South Korean cities. A cross-sectional examination of urine phthalate concentrations was performed, and scores on neuropsychological tests were obtained from both the children and their mothers. We measured mono-2-ethylhexyl phthalate (MEHP) and mono(2-ethyl-5-oxohexyl)phthalate (MEOHP), both metabolites of di(2-ethylhexyl)phthalate (DEHP), and mono-n-butyl phthalate (MBP), a metabolite of dibutyl phthalate (DBP), in urine samples. The geometric mean (ln) concentrations of MEHP, MEOHP, and MBP were 21.3 microg/L [geometric SD (GSD) = 2.2 microg/L; range, 0.5-445.4], 18.0 microg/L (GSD = 2.4; range, 0.07-291.1), and 48.9 microg/L (GSD = 2.2; range, 2.1-1645.5), respectively. After adjusting for demographic and developmental covariates, the Full Scale IQ and Verbal IQ scores were negatively associated with DEHP metabolites but not with DBP metabolites. We also found a significant negative relationship between the urine concentrations of the metabolites of DEHP and DBP and children's vocabulary subscores. After controlling for maternal IQ, a significant inverse relationship between DEHP metabolites and vocabulary subscale score remained. Among boys, we found a negative association between increasing MEHP phthalate concentrations and the sum of DEHP metabolite concentrations and Wechsler Intelligence Scale for Children vocabulary score; however, among girls, we found no significant association between these variables. Controlling for maternal IQ and other covariates, the results show an inverse relationship between phthalate metabolites and IQ scores; however, given the limitations in cross-sectional epidemiology, prospective studies are needed to fully explore these associations.

Demography and disorders of German Shepherd Dogs under primary veterinary care in the UK.

PubMed

O'Neill, Dan G; Coulson, Noel R; Church, David B; Brodbelt, Dave C

2017-01-01

The German Shepherd Dog (GSD) has been widely used for a variety of working roles. However, concerns for the health and welfare of the GSD have been widely aired and there is evidence that breed numbers are now in decline in the UK. Accurate demographic and disorder data could assist with breeding and clinical prioritisation. The VetCompass TM Programme collects clinical data on dogs under primary veterinary care in the UK. This study included all VetCompass TM dogs under veterinary care during 2013. Demographic, mortality and clinical diagnosis data on GSDs were extracted and reported. GSDs dropped from 3.5% of the annual birth cohort in 2005 to 2.2% in 2013. The median longevity of GSDs was 10.3 years (IQR 8.0-12.1, range 0.2-17.0). The most common causes of death were musculoskeletal disorder (16.3%) and inability to stand (14.9%). The most prevalent disorders recorded were otitis externa ( n  = 131, 7.89, 95% CI: 6.64-9.29), osteoarthritis (92, 5.54%, 95% CI: 4.49-6.75), diarrhoea (87, 5.24%, 95% CI: 4.22-6.42), overweight/obesity (86, 5.18%, 95% CI: 4.16-6.36) and aggression (79, 4.76%, 95% CI: 3.79-5.90). This study identified that GSDs have been reducing in numbers in the UK in recent years. The most frequent disorders in GSDs were otitis externa, osteoarthritis, diarrhoea, overweight/obesity and aggression, whilst the most common causes of death were musculoskeletal disorders and inability to stand. Aggression was more prevalent in males than in females. These results may assist veterinarians to offer evidence-based advice at a breed level and help to identify priorities for GSD health that can improve the breed's health and welfare.

A Quantitative Study of Global Software Development Teams, Requirements, and Software Projects

ERIC Educational Resources Information Center

Parker, Linda L.

2016-01-01

The study explored the relationship between global software development teams, effective software requirements, and stakeholders' perception of successful software development projects within the field of information technology management. It examined the critical relationship between Global Software Development (GSD) teams creating effective…

Effect of lime concentration on gelatinized maize starch dispersions properties.

PubMed

Lobato-Calleros, C; Hernandez-Jaimes, C; Chavez-Esquivel, G; Meraz, M; Sosa, E; Lara, V H; Alvarez-Ramirez, J; Vernon-Carter, E J

2015-04-01

Maize starch was lime-cooked at 92 °C with 0.0-0.40% w/w Ca(OH)2. Optical micrographs showed that lime disrupted the integrity of insoluble remnants (ghosts) and increased the degree of syneresis of the gelatinized starch dispersions (GSD). The particle size distribution was monomodal, shifting to smaller sizes and narrower distributions with increasing lime concentration. X-ray patterns and FTIR spectra showed that crystallinity decreased to a minimum at lime concentration of 0.20% w/w. Lime-treated GSD exhibited thixotropic and viscoelastic behaviour. In the linear viscoelastic region the storage modulus was higher than the loss modulus, but a crossover between these moduli occurred in the non-linear viscoelastic region. The viscoelastic properties decreased with increased lime concentration. The electrochemical properties suggested that the amylopectin-rich remnants and the released amylose contained in the continuous matrix was firstly attacked by calcium ions at low lime levels (<0.20% w/w), disrupting the starch gel microstructure. Copyright © 2014 Elsevier Ltd. All rights reserved.

Soil mechanics: breaking ground.

PubMed

Einav, Itai

2007-12-15

In soil mechanics, student's models are classified as simple models that teach us unexplained elements of behaviour; an example is the Cam clay constitutive models of critical state soil mechanics (CSSM). 'Engineer's models' are models that elaborate the theory to fit more behavioural trends; this is usually done by adding fitting parameters to the student's models. Can currently unexplained behavioural trends of soil be explained without adding fitting parameters to CSSM models, by developing alternative student's models based on modern theories?Here I apply an alternative theory to CSSM, called 'breakage mechanics', and develop a simple student's model for sand. Its unique and distinctive feature is the use of an energy balance equation that connects grain size reduction to consumption of energy, which enables us to predict how grain size distribution (gsd) evolves-an unprecedented capability in constitutive modelling. With only four parameters, the model is physically clarifying what CSSM cannot for sand: the dependency of yielding and critical state on the initial gsd and void ratio.

NAOMI instrument: a product line of compact and versatile cameras designed for high resolution missions in Earth observation

NASA Astrophysics Data System (ADS)

Luquet, Ph.; Chikouche, A.; Benbouzid, A. B.; Arnoux, J. J.; Chinal, E.; Massol, C.; Rouchit, P.; De Zotti, S.

2017-11-01

EADS Astrium is currently developing a new product line of compact and versatile instruments for high resolution missions in Earth Observation. First version has been developed in the frame of the ALSAT-2 contract awarded by the Algerian Space Agency (ASAL) to EADS Astrium. The Silicon Carbide Korsch-type telescope coupled with a multilines detector array offers a 2.5 m GSD in PAN band at Nadir @ 680 km altitude (10 m GSD in the four multispectral bands) with a 17.5 km swath width. This compact camera - 340 (W) x 460 (L) x 510 (H) mm3, 13 kg - is embarked on a Myriade-type small platform. The electronics unit accommodates video, housekeeping, and thermal control functions and also a 64 Gbit mass memory. Two satellites are developed; the first one is planned to be launched on mid 2009. Several other versions of the instrument have already been defined with enhanced resolution or/and larger field of view.

Estimating the Economic Value of National Parks with Count Data Models Using On-Site, Secondary Data: The Case of the Great Sand Dunes National Park and Preserve

NASA Astrophysics Data System (ADS)

Heberling, Matthew T.; Templeton, Joshua J.

2009-04-01

We estimate an individual travel cost model for Great Sand Dunes National Park and Preserve (GSD) in Colorado using on-site, secondary data. The purpose of the on-site survey was to help the National Park Service better understand the visitors of GSD; it was not intended for a travel cost model. Variables such as travel cost and income were estimated based on respondents’ Zip Codes. Following approaches found in the literature, a negative binomial model corrected for truncation and endogenous stratification fit the data the best. We estimate a recreational benefit of U.S. 89/visitor/year or U.S. 54/visitor/24-h recreational day (in 2002 U.S. ). Based on the approach presented here, there are other data sets for national parks, preserves, and battlefields where travel cost models could be estimated and used to support National Park Service management decisions.

CORRELATION OF FLORIDA SOIL-GAS PERMEABILITIES WITH GRAIN SIZE, MOISTURE, AND POROSITY

EPA Science Inventory

The report describes a new correlation or predicting gas permeabilities of undisturbed or recompacted soils from their average grain diameter (d), moisture saturation factor (m), and porosity (p). he correlation exhibits a geometric standard deviation (GSD) of only 1.27 between m...

75 FR 66319 - State Systems Advance Planning Document (APD) Process

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-28

...) equipment and services. The APD process was designed to mitigate financial risks, avoid incompatibilities... develop a General Systems Design (GSD). Implementation APD means a recorded plan of action to request Federal financial participation (FFP) in the costs of designing, developing and implementing the system...

Characterizing 3D grain size distributions from 2D sections in mylonites using a modified version of the Saltykov method

NASA Astrophysics Data System (ADS)

Lopez-Sanchez, Marco; Llana-Fúnez, Sergio

2016-04-01

The understanding of creep behaviour in rocks requires knowledge of 3D grain size distributions (GSD) that result from dynamic recrystallization processes during deformation. The methods to estimate directly the 3D grain size distribution -serial sectioning, synchrotron or X-ray-based tomography- are expensive, time-consuming and, in most cases and at best, challenging. This means that in practice grain size distributions are mostly derived from 2D sections. Although there are a number of methods in the literature to derive the actual 3D grain size distributions from 2D sections, the most popular in highly deformed rocks is the so-called Saltykov method. It has though two major drawbacks: the method assumes no interaction between grains, which is not true in the case of recrystallised mylonites; and uses histograms to describe distributions, which limits the quantification of the GSD. The first aim of this contribution is to test whether the interaction between grains in mylonites, i.e. random grain packing, affects significantly the GSDs estimated by the Saltykov method. We test this using the random resampling technique in a large data set (n = 12298). The full data set is built from several parallel thin sections that cut a completely dynamically recrystallized quartz aggregate in a rock sample from a Variscan shear zone in NW Spain. The results proved that the Saltykov method is reliable as long as the number of grains is large (n > 1000). Assuming that a lognormal distribution is an optimal approximation for the GSD in a completely dynamically recrystallized rock, we introduce an additional step to the Saltykov method, which allows estimating a continuous probability distribution function of the 3D grain size population. The additional step takes the midpoints of the classes obtained by the Saltykov method and fits a lognormal distribution with a trust region using a non-linear least squares algorithm. The new protocol is named the two-step method. The conclusion of this work is that both the Saltykov and the two-step methods are accurate and simple enough to be useful in practice in rocks, alloys or ceramics with near-equant grains and expected lognormal distributions. The Saltykov method is particularly suitable to estimate the volumes of particular grain fractions, while the two-step method to quantify the full GSD (mean and standard deviation in log grain size). The two-step method is implemented in a free, open-source and easy-to-handle script (see http://marcoalopez.github.io/GrainSizeTools/).

On the creation of high spatial resolution imaging spectroscopy data from multi-temporal low spatial resolution imagery

NASA Astrophysics Data System (ADS)

Yao, Wei; van Aardt, Jan; Messinger, David

2017-05-01

The Hyperspectral Infrared Imager (HyspIRI) mission aims to provide global imaging spectroscopy data to the benefit of especially ecosystem studies. The onboard spectrometer will collect radiance spectra from the visible to short wave infrared (VSWIR) regions (400-2500 nm). The mission calls for fine spectral resolution (10 nm band width) and as such will enable scientists to perform material characterization, species classification, and even sub-pixel mapping. However, the global coverage requirement results in a relatively low spatial resolution (GSD 30m), which restricts applications to objects of similar scales. We therefore have focused on the assessment of sub-pixel vegetation structure from spectroscopy data in past studies. In this study, we investigate the development or reconstruction of higher spatial resolution imaging spectroscopy data via fusion of multi-temporal data sets to address the drawbacks implicit in low spatial resolution imagery. The projected temporal resolution of the HyspIRI VSWIR instrument is 15 days, which implies that we have access to as many as six data sets for an area over the course of a growth season. Previous studies have shown that select vegetation structural parameters, e.g., leaf area index (LAI) and gross ecosystem production (GEP), are relatively constant in summer and winter for temperate forests; we therefore consider the data sets collected in summer to be from a similar, stable forest structure. The first step, prior to fusion, involves registration of the multi-temporal data. A data fusion algorithm then can be applied to the pre-processed data sets. The approach hinges on an algorithm that has been widely applied to fuse RGB images. Ideally, if we have four images of a scene which all meet the following requirements - i) they are captured with the same camera configurations; ii) the pixel size of each image is x; and iii) at least r2 images are aligned on a grid of x/r - then a high-resolution image, with a pixel size of x/r, can be reconstructed from the multi-temporal set. The algorithm was applied to data from NASA's classic Airborne Visible and Infrared Imaging Spectrometer (AVIRIS-C; GSD 18m), collected between 2013-2015 (summer and fall) over our study area (NEON's Southwest Pacific Domain; Fresno, CA) to generate higher spatial resolution imagery (GSD 9m). The reconstructed data set was validated via comparison to NEON's imaging spectrometer (NIS) data (GSD 1m). The results showed that algorithm worked well with the AVIRIS-C data and could be applied to the HyspIRI data.

Low Cost Micro-Mini-Satellite Remote Sensing Capabilities: in-Orbit Results &Imminent Missions

NASA Astrophysics Data System (ADS)

Stephens, Paul; Sun, Wei; Sweeting, Martin, , Sir

Micro- and mini-satellites are in the process or revolutionising the economics of Earth observation. This will jointly affect the space super-powers who have, since the dawn of the space age, enjoyed an effective monopoly of Earth observation from the high vantage-point of space and also the commercial provision of EO data to value added information producers. The monopoly has been due to the enormous cost hitherto required to build, launch and operate EO satellites. SSTL (UK) has pioneered the development of successful micro and mini-satellites which have demonstrated highly capable Earth Observation functions at a mission cost at least an order of magnitude less than conventional such missions. This dramatic development has brought independent ownership of Earth observation satellites within the affordable reach of every developing nation and even medium-sized commercial concerns. Indeed, the performance of these tiny satellites now exceeds the capability of many of the civil EO satellites in operation only 5 years ago. In 2002, SSTL will launch the first satellite in a constellation that will deliver the first routine 24-hour revisit EO data released into the commercial marketplace. This paper describes the in-orbit EO image data produced by typical micro and minisatellites including the latest imagery from the UoSAT-12 mini satellite launched in April 1999 which carries a 32-metre ground sampling distance multispectral imager and a 10-metre GSD panchromatic camera. In addition, data is presented from the TiungSat-1 and Tsinghua-1 microsatellites launched in 2000, and AlSat-1 (launch scheduled in September 2002). AlSat-1 carries a unique imaging system designed as part of the innovative Disaster Monitoring Constellation providing 32-metre GSD multispectral images with a 600km swath width - together with its five companion microsatellites, the Disaster Monitoring Constellation can provide daily revisit imaging world-wide from orbit. The paper also describes the latest advances in imaging using two new low cost small satellites being built at Surrey. The commercial RapidEye constellation of 4-6 microsatellites is designed to provide daily imaging at 6.5-metre GSD in 6 spectral band over Europe for precision farming applications. At a higher resolution, TopSat is a pilot mission for the UK Ministry of Defence carrying a 2.5-metre GSD panchromatic camera on a highly agile microsatellite, designed to deliver images direct to mobile groundstations in the field.

GSD Update: Checking the range for signs of climate change in the past, present and future

Treesearch

Lisa-Natalie Anjozian

2011-01-01

The July 2011 inaugural issue of GSDUpdate: Checking the Range for Signs of Climate Change In the Past, Present and Future, a research review of the Program, focuses on the efforts toward understanding the role of climate in shaping the environment.

75 FR 33871 - Self-Regulatory Organizations; Fixed Income Clearing Corporation; Notice of Filing and Immediate...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-15

...-Regulatory Organizations; Fixed Income Clearing Corporation; Notice of Filing and Immediate Effectiveness of... is hereby given that on May 28, 2010, the Fixed Income Clearing Corporation (``FICC'') filed with the... Division's (``GSD'') rules pertaining to the Required Fund Deposit Deadline. II. Self-Regulatory...

GSD Update: Strategies for understanding and controlling species invasions

Treesearch

Deborah M. Finch

2013-01-01

Invasive species are significant environmental and economic threats to our Nation's and the world's ecosystems and natural resources. They impact wildlife habitat, endanger native species, threaten ecosystem services, damage lakes and rivers, reduce recreational opportunities, affect production of wood products, jeopardize human health and safety and reduce...

Biological availability of (238)U, (234)U and (226)Ra for wild berries and meadow grasses in natural ecosystems of Belarus.

PubMed

Sokolik, G A; Ovsiannikova, S V; Voinikava, K V; Ivanova, T G; Papenia, M V

2014-01-01

This work is devoted to investigation of behavior of (234)U, (238)U and (226)Ra by determining the soil to plant transfer under different natural conditions such as forest or swamped areas and meadow lands with different soil types. The paper summarizes the data on investigation of uranium and radium uptake by wild berries and natural meadow grasses in the typical conditions of Belarus. Parameters characterizing the biological availability of (234)U, (238)U and (226)Ra for bilberry (Vaccinium myrtillus), lingonberry (Vaccinium viti-idaea), blueberry (Vaccinium iliginosum) and cranberry (Vaccinium oxycoccus palustris) as well as for widely occurring mixed meadow vegetation, which belongs to the sedge-grass or grass-sedge associations and forbs, have been established. In the sites under investigation, the deposition levels of (238+239+240)Pu were less than 0.37 kBq m(-2) and (137)Cs deposition ranged between less than 0.37 and 37 kBq m(-2). It was found that activity concentrations of radionuclides in berries varied in the ranges of 0.037-0.11 for (234)U, 0.036-0.10 for (238)U and 0.11-0.43 Bq kg(-1) for (226)Ra, but in the mixed meadow grasses they were 0.32-4.4, 0.24-3.9 and 0.14-6.9 Bq kg(-1) accordingly. The (234)U/(238)U activity ratios were 1.02 ± 0.01 for wild berries, 1.20 ± 0.09 for underground meadow grasses and 1.02 ± 0.02 for proper soils. The concentration ratios (CRs, dry weight basis) of (234)U and (238)U for mixed meadow grasses were 0.036-0.42 and 0.041-0.46 respectively. The correspondent geometric means (GM) were 0.13 and 0.15 with geometric standard deviations (GSD) of 2.4. The CRs of (226)Ra for meadow grasses were 0.031-1.0 with GM 0.20 and GSD 2.6. The CRs of (234)U, (238)U and (226)Ra for wild berries ranged within 0.0018-0.008 (GM is 0.0034, GSD is 1.8), 0.0018-0.008 (GM is 0.0035, GSD is 1.8) and 0.005-0.033 (GM is 0.016, GSD is 2.1) accordingly. The highest CR values of uranium for mixed meadow grasses were found in the sites with high-moor peat and sandy soils. The lowest CRs for grasses were common to loamy and peat-gley soils. The CRs for the same berry species in the sites with sandy soils exceeded the appropriate CR values in the sites with loamy soils by factors of 3-4 for uranium and 4-6 for radium. The data obtained on radionuclide accumulation by plants were used to estimate the average annual effective doses to the population from radionuclide intake through the "soil - wild berries - man" and "soil - meadow vegetation - animal - cow milk-beef - man" trophic chains. The effective doses resulting from (234)U, (238)U and (226)Ra intake with the wild berries for adults were estimated as 0.02-0.09 μSv y(-1) (GM is 0.044, GSD is 1.6). It was established that only in the territory with (137)Cs deposition of ∼1.0-1.5 kBq m(-2) the doses resulting from (234)U, (238)U and (226)Ra intake with wild berries can be comparable with corresponding doses from (137)Cs. In the territories with higher levels of (137)Cs deposition the doses resulting from its intake with the wild berries are usually over the summarized doses of uranium and (226)Ra. The total doses for adults resulting from uranium and (226)Ra intake with cow milk and beef ranged between 0.2 and 7.2 μSv y(-1) (GM is 2.0; GSD is 2.9) and the doses from (226)Ra usually exceeded the appropriate doses of uranium with a factor of 3-37. In the sites with (137)Cs deposition less than 3.7 kBq m(-2), the doses from (234)U, (238)U and (226)Ra intake with cow milk and beef were assessing as 1.1-7.2 μSv y(-1) and they were usually higher than the doses from (137)Cs, which were assessing as 0.4-3.2 μSv y(-1) for its deposition 2 kBq m(-2). In the territory with (137)Cs deposition 10-20 kBq m(-2) and higher, the internal doses resulting from (137)Cs intake with cow milk and beef (10-50 μSv y(-1)) exceeded the proper doses from natural (234)U, (238)U and (226)Ra. Copyright © 2013 Elsevier Ltd. All rights reserved.

Temperature, Genes, and Sex: a Comparative View of Sex Determination in Trachemys scripta and Mus musculus

PubMed Central

Yao, Humphrey H-C; Capel, Blanche

2014-01-01

Sex determination, the step at which differentiation of males and females is initiated in the embryo, is of central importance to the propagation of species. There is a remarkable diversity of mechanisms by which sex determination is accomplished. In general these mechanisms fall into two categories: Genetic Sex Determination (GSD), which depends on genetic differences between the sexes, and Environmental Sex Determination (ESD), which depends on extrinsic cues. In this review we will consider these two means of determining sex with particular emphasis on two species: a species that depends on GSD, Mus musculus, and a species that depends on ESD, Trachemys scripta. Because the structural organization of the adult testis and ovary is very similar across vertebrates, most biologists had expected that the pathways downstream of the sex-determining switch would be conserved. However, emerging data indicate that not only are the initial sex determining mechanisms different, but the downstream pathways and morphogenetic events leading to the development of a testis or ovary also are different. PMID:16046442

Hyperspectral signatures and WorldView-3 imagery of Indian River Lagoon and Banana River Estuarine water and bottom types

NASA Astrophysics Data System (ADS)

Bostater, Charles R.; Oney, Taylor S.; Rotkiske, Tyler; Aziz, Samin; Morrisette, Charles; Callahan, Kelby; Mcallister, Devin

2017-10-01

Hyperspectral signatures and imagery collected during the spring and summer of 2017 and 2016 are presented. Ground sampling distances (GSD) and pixel sizes were sampled from just over a meter to less than 4.0 mm. A pushbroom hyperspectral imager was used to calculate bidirectional reflectance factor (BRF) signatures. Hyperspectral signatures of different water types and bottom habitats such as submerged seagrasses, drift algae and algal bloom waters were scanned using a high spectral and digital resolution solid state spectrograph. WorldView-3 satellite imagery with minimal water wave sun glint effects was used to demonstrate the ability to detect bottom features using a derivative reflectance spectroscopy approach with the 1.3 m GSD multispectral satellite channels centered at the solar induced fluorescence band. The hyperspectral remote sensing data collected from the Banana River and Indian River Lagoon watersheds represents previously unknown signatures to be used in satellite and airborne remote sensing of water in turbid waters along the US Atlantic Ocean coastal region and the Florida littoral zone.

Rubella antibodies in Australian immunoglobulin products.

PubMed

Young, Megan K; Bertolini, Joseph; Kotharu, Pushpa; Maher, Darryl; Cripps, Allan W

2017-08-03

Rubella antibodies are not routinely measured in immunoglobulin products and there is a lack of information on the titer in Australian products. To facilitate future studies of the effectiveness of passive immunisation for preventing rubella and congenital rubella syndrome, this study measured the concentration of rubella-specific antibodies in Australian intramuscular (IM) and intravenous (IV) human immunoglobulin products suitable for post-exposure prophylaxis using a chemiluminescent immunoassay. The GMT ± GSD for the IM product was 19 ± 1.2 IU/mg (2980 ± 1.2 IU/mL). The GMT ± GSD for the IV product was 12 ± 1.5 IU/mg (729 ± 1.5 IU/mL). At present, Australian guidelines recommend offering non-immune pregnant women exposed to rubella 20 mL of intramuscular immunoglobulin within 72 hours of exposure. This equates to 42,160 IU of rubella antibodies if the lowest titer obtained for the Australian IM product is considered. The same dose would be delivered by 176 mL of the Australian IV product at the lowest measured rubella-specific antibody titer.

Sharpening advanced land imager multispectral data using a sensor model

USGS Publications Warehouse

Lemeshewsky, G.P.; ,

2005-01-01

The Advanced Land Imager (ALI) instrument on NASA's Earth Observing One (EO-1) satellite provides for nine spectral bands at 30m ground sample distance (GSD) and a 10m GSD panchromatic band. This report describes an image sharpening technique where the higher spatial resolution information of the panchromatic band is used to increase the spatial resolution of ALI multispectral (MS) data. To preserve the spectral characteristics, this technique combines reported deconvolution deblurring methods for the MS data with highpass filter-based fusion methods for the Pan data. The deblurring process uses the point spread function (PSF) model of the ALI sensor. Information includes calculation of the PSF from pre-launch calibration data. Performance was evaluated using simulated ALI MS data generated by degrading the spatial resolution of high resolution IKONOS satellite MS data. A quantitative measure of performance was the error between sharpened MS data and high resolution reference. This report also compares performance with that of a reported method that includes PSF information. Preliminary results indicate improved sharpening with the method reported here.

Characterization of the mutations in the glucose-6-phosphatase gene in Israeli patients with glycogen storage disease type 1a: R83C in six Jews and a novel V166G mutation in a Muslim Arab.

PubMed

Parvari, R; Moses, S; Hershkovitz, E; Carmi, R; Bashan, N

1995-01-01

Glycogen storage disease type 1a (GSD 1a), an autosomal recessive disease, is caused by the inactivity of glucose-6-phosphatase, the gene of which has been recently cloned. We report on the missense mutation C-->T at nucleotide 326 of the G6Pase gene, causing the change of the Arg codon at position 83 into a Cys codon, as the single mutation detected in six Jewish patients. This finding suggests that this mutation might be prevalent among the Jewish population. A new missense mutation T-->G at nucleotide 576 resulting in V166G was found in an Arab Muslim patient. These families may benefit now from pre- and postnatal diagnosis by analysis of DNA from blood and amniotic fluid or chorionic villus cells rather than liver biopsy. No mutations in the G6Pase gene were detected in two GSD 1b patients.

Two new mutations in the 3' coding region of the glycogen debranching enzyme in a glycogen storage disease type IIIa Ashkenazi Jewish patient.

PubMed

Parvari, R; Shen, J; Hershkovitz, E; Chen, Y T; Moses, S W

1998-04-01

Glycogen storage disease type III (GSD III) is an autosomal recessive disease caused by the deficiency of glycogen debranching enzyme (AGL). We report the finding of two new mutations in a GSD IIIa Ashkenazi Jewish patient. Both mutations are insertion of an adenine into a stretch of 8 adenines towards the 3' end of the coding region, one at position 3904 (3904insA) in exon 30, the second at position 4214 (4214insA) in exon 32. The mutations cause frameshifts and premature terminations of the glycogen debranching enzyme, the first causing a frameshift at amino acid 1304, the second causing a frameshift at amino acid 1408 of the total of 1532. These mutations demonstrate the importance of the 125 amino acids at the carboxy-terminus of the debrancher enzyme for its activity and support the suggestion that the putative glycogen binding domain is located in the carboxy-terminus of the AGL. The mutations cause distinctive single-strand conformation polymorphism (SSCP) patterns enabling easy detection.

Mutations in the glucose-6-phosphatase gene that cause glycogen storage disease type 1a

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chou, J.Y.; Lei, K.J.; Shelly, L.L.

1994-09-01

Glycogen storage disease (GSD) type la (von Gierke disease) is caused by the deficiency of glucose-6-phosphatase (G6Pase), the key enzyme in glucose homeostasis. The disease presents with clinical manifestations of severe hypoglycemia, hepatomegaly, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. We have succeeded in isolating a murine G6Pase cDNA from a normal mouse liver cDNA library by differentially screening method. We then isolated the human G6Pase cDNA and gene. To date, we have characterized the G6Pase genes of twelve GSD type la patients and uncovered a total of six different mutations. The mutations are comprised of R83C (an Arg atmore » codon 83 to a Cys), Q347X (a Gly at codon 347 to a stop codon), 459insTA (a two basepair insertion at nucleotide 459 yielding a truncated G6Pase of 129 residues), R295C (an Arg at codon 295 to a Cys), G222R (a Gly at codon 222 to an Arg) and {delta}F327 (a codon deletion for Phe-327 at nucleotides 1058 to 1060). The relative incidences of these mutations are 37.5% (R83C), 33.3% (Q347X), 16.6% (459insTA), 4.2% (G222R), 4.2% (R295C) and 4.2% ({delta}F327). Site-directed mutagenesis and transient expression assays demonstrated that the R83C, Q347X, R295C, and {delta}F327 mutations abolished whereas the G222R mutation greatly reduced G6Pase activity. We further characterized the structure-function requirements of amino acids 83, 222, and 295 in G6Pase catalysis. The identification of mutations in GSD type la patients has unequivocally established the molecular basis of the type la disorder. Knowledge of the mutations may be applied to prenatal diagnosis and opens the way for developing and evaluating new therapeutic approaches.« less

Estimating Temperature Retrieval Accuracy Associated With Thermal Band Spatial Resolution Requirements for Center Pivot Irrigation Monitoring and Management

NASA Technical Reports Server (NTRS)

Ryan, Robert E.; Irons, James; Spruce, Joseph P.; Underwood, Lauren W.; Pagnutti, Mary

2006-01-01

This study explores the use of synthetic thermal center pivot irrigation scenes to estimate temperature retrieval accuracy for thermal remote sensed data, such as data acquired from current and proposed Landsat-like thermal systems. Center pivot irrigation is a common practice in the western United States and in other parts of the world where water resources are scarce. Wide-area ET (evapotranspiration) estimates and reliable water management decisions depend on accurate temperature information retrieval from remotely sensed data. Spatial resolution, sensor noise, and the temperature step between a field and its surrounding area impose limits on the ability to retrieve temperature information. Spatial resolution is an interrelationship between GSD (ground sample distance) and a measure of image sharpness, such as edge response or edge slope. Edge response and edge slope are intuitive, and direct measures of spatial resolution are easier to visualize and estimate than the more common Modulation Transfer Function or Point Spread Function. For these reasons, recent data specifications, such as those for the LDCM (Landsat Data Continuity Mission), have used GSD and edge response to specify spatial resolution. For this study, we have defined a 400-800 m diameter center pivot irrigation area with a large 25 K temperature step associated with a 300 K well-watered field surrounded by an infinite 325 K dry area. In this context, we defined the benchmark problem as an easily modeled, highly common stressing case. By parametrically varying GSD (30-240 m) and edge slope, we determined the number of pixels and field area fraction that meet a given temperature accuracy estimate for 400-m, 600-m, and 800-m diameter field sizes. Results of this project will help assess the utility of proposed specifications for the LDCM and other future thermal remote sensing missions and for water resource management.

3D Micro-tomography on Aggregates from the 2014- 2015 Eruption of Hunga Tonga-Hunga Ha'apai Volcano

NASA Astrophysics Data System (ADS)

Colombier, M.; Scheu, B.; Cronin, S. J.; Tost, M.; Dobson, K. J.; Dingwell, D. B.

2016-12-01

In December 2014- January 2015, a surtseyan eruption at Hunga Tonga-Hunga Ha'apai volcano (Tonga) formed a new island. Three main eruptive phases were distinguished by observation and deposits: (i) mound and cone construction, involving collapse of 300-600 m-high wet tephra jets, grain flows, slope-remobilisation and energetic surges, with little or no convective plume (ii) The upper cone-building phase with lower jets (mainly <300 m) but greater ash production (weak, steam-rich plumes to 6 km) and weak surges, and (iii) final phase with weak surge, fall and ballistic deposits with more vesicular pyroclasts producing proximal capping deposits. Most sampled deposits contain ash, lapilli and bombs, and lapilli-sized aggregates are ubiquitous. We used high-resolution 3D X-ray microcomputed tomography (XCT) to quantify the grain size distribution (GSD) and porosity by sampling multiple stratigraphic units within the main eruptive sequences. We visualized and quantified the internal structure of the aggregates to understand the evolution of this surtseyan eruption. We present here an overview of the textural information: porosity, vesicle size distribution and morphology as well as the variability of the aggregation features. Aggregates from the fall deposits of the early wet phase are mostly loosely packed, poorly-structured ash clusters. Aggregates from the early surge sequence and the main cone building phase dominantly exhibit a central particle coated by ash cluster material. Vesicles in the particles from the early fall deposits tend to be smaller and more isolated than in the particles from the surge sequence and the main cone building phase. The GSD of aggregates obtained by XCT is highly valuable to correct the total GSD of volcaniclastic deposits. The strong variations in the aggregation features across the eruption suggest a range of different formation and deposition mechanisms related to varying degrees of magma-water-interaction, which changed the morphology and textural properties of the individual particles.

Relationship between Environmental Phthalate Exposure and the Intelligence of School-Age Children

PubMed Central

Cho, Soo-Churl; Bhang, Soo-Young; Hong, Yun-Chul; Shin, Min-Sup; Kim, Boong-Nyun; Kim, Jae-Won; Yoo, Hee-Jung; Cho, In Hee; Kim, Hyo-Won

2010-01-01

Background Concern over phthalates has emerged because of their potential toxicity to humans. Objective We investigated the relationship between the urinary concentrations of phthalate metabolites and children’s intellectual functioning. Methods This study enrolled 667 children at nine elementary schools in five South Korean cities. A cross-sectional examination of urine phthalate concentrations was performed, and scores on neuropsychological tests were obtained from both the children and their mothers. Results We measured mono-2-ethylhexyl phthalate (MEHP) and mono(2-ethyl-5-oxohexyl)phthalate (MEOHP), both metabolites of di(2-ethylhexyl)phthalate (DEHP), and mono-n-butyl phthalate (MBP), a metabolite of dibutyl phthalate (DBP), in urine samples. The geometric mean (ln) concentrations of MEHP, MEOHP, and MBP were 21.3 μg/L [geometric SD (GSD) = 2.2 μg/L; range, 0.5–445.4], 18.0 μg/L (GSD = 2.4; range, 0.07–291.1), and 48.9 μg/L (GSD = 2.2; range, 2.1–1645.5), respectively. After adjusting for demographic and developmental covariates, the Full Scale IQ and Verbal IQ scores were negatively associated with DEHP metabolites but not with DBP metabolites. We also found a significant negative relationship between the urine concentrations of the metabolites of DEHP and DBP and children’s vocabulary subscores. After controlling for maternal IQ, a significant inverse relationship between DEHP metabolites and vocabulary subscale score remained. Among boys, we found a negative association between increasing MEHP phthalate concentrations and the sum of DEHP metabolite concentrations and Wechsler Intelligence Scale for Children vocabulary score; however, among girls, we found no significant association between these variables. Conclusion Controlling for maternal IQ and other covariates, the results show an inverse relationship between phthalate metabolites and IQ scores; however, given the limitations in cross-sectional epidemiology, prospective studies are needed to fully explore these associations. PMID:20194078

Radiometric and geometric characteristics of Pleiades images

NASA Astrophysics Data System (ADS)

Jacobsen, K.; Topan, H.; Cam, A.; Özendi, M.; Oruc, M.

2014-11-01

Pleiades images are distributed with 50 cm ground sampling distance (GSD) even if the physical resolution for nadir images is just 70 cm. By theory this should influence the effective GSD determined by means of point spread function at image edges. Nevertheless by edge enhancement the effective GSD can be improved, but this should cause enlarged image noise. Again image noise can be reduced by image restoration. Finally even optimized image restoration cannot improve the image information from 70 cm to 50 cm without loss of details, requiring a comparison of Pleiades image details with other very high resolution space images. The image noise has been determined by analysis of the whole images for any sub-area with 5 pixels times 5 pixels. Based on the standard deviation of grey values in the small sub-areas the image noise has been determined by frequency analysis. This leads to realistic results, checked by test targets. On the other hand the visual determination of image noise based on apparently homogenous sub-areas results in too high values because the human eye is not able to identify small grey value differences - it is limited to just approximately 40 grey value steps over the available gray value range, so small difference in grey values cannot be seen, enlarging results of a manual noise determination. A tri-stereo combination of Pleiades 1A in a mountainous, but partially urban, area has been analyzed and compared with images of the same area from WorldView-1, QuickBird and IKONOS. The image restoration of the Pleiades images is very good, so the effective image resolution resulted in a factor 1.0, meaning that the effective resolution corresponds to the nominal resolution of 50 cm. This does not correspond to the physical resolution of 70 cm, but by edge enhancement the steepness of the grey value profile across the edge can be enlarged, reducing the width of the point spread function. Without additional filtering edge enhancement enlarges the image noise, but the average image noise of approximately 1.0 grey values related to 8 bit images is very small, not indicating the edge enhancement and the down sampling of the GSD from 70 cm to 50 cm. So the direct comparison with the other images has to give the answer if the image quality of Pleiades images is on similar level as corresponding to the nominal resolution. As expected with the image geometry there is no problem. This is the case for all used space images in the test area, where the point identification limits the accuracy of the scene orientation.

GSD Update: Ushering in a new age of genetics to restore lands and conserve species

Treesearch

Deborah M. Finch

2013-01-01

Plant genetic information provides critical knowledge necessary to mitigate the impacts of climate change through ecological restoration. The first step in restoration is recognizing and delineating genetic boundaries at different taxonomic and spatial hierarchies (e.g., species, subspecies and populations). The second step is an assessment of the genetic diversity...

Tamarisk Mapping and Monitoring Using High Resolution Satellite Imagery

Treesearch

Jason W. San Souci; John T. Doyle

2006-01-01

QuickBird high resolution multispectral satellite imagery (60 cm GSD, 4 spectral bands) and calibrated products from DigitalGlobe’s AgroWatch program were used as inputs to Visual Learning System’s Feature Analyst automated feature extraction software to map localized occurrences of pervasive and aggressive Tamarisk (Tamarix ramosissima), an invasive...

GSD Update: The West in transition: Costs and unexpected benefits of disrupting ecosystems

Treesearch

Dan Ferber

2012-01-01

For centuries, the resilience of western ecosystems kept pace with changes in climate, native species and peoples, and other natural stressors. Droughts, winds, floods, insect outbreaks, bison, and wild fires periodically disrupted the ecosystem dynamics of America's grasslands, shrublands and deserts, and the changes would sometimes last a few years. Native...

Progress and Backlash in the Wake of "Obergefell": Reaching Conservative Southern Teachers through the Power of Literature

ERIC Educational Resources Information Center

Beck, Scott A.; Walker-DeVose, Dina; Agnich, Laura E.; Town, Caren; Smith, Trina

2017-01-01

This paper uses a mixed narrative and quantitative analysis to examine how a graduate class of predominantly politically and religiously conservative (self-identified), elementary teachers in the South made discursive sense of gender and sexually diverse (GSD) young adult and children's literature in the context of concurrent, relevant national…

77 FR 20445 - Self-Regulatory Organizations; Fixed Income Clearing Corporation; Notice of Filing of Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-04

... Professionals'' March 29, 2012. Pursuant to Section 19(b)(1) of the Securities Exchange Act of 1934 (``Act'') \\1... positions held by GSD Netting Members and NYPC Clearing Members for certain ``market professionals.'' \\5\\ \\4...\\ The NYPC-FICC ``market professional'' cross-margining program aims to closely replicate the Options...

77 FR 15155 - Self-Regulatory Organizations; Fixed Income Clearing Corporation; Order Approving Amended...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-14

... Company Act of 1940 and have minimum net assets of $100 million. In addition to standard requirements... Advisors Act of 1940; and the UIP must have (i) $250 million in net assets, or (ii) $100 million in net... apply to certain member types. For example, the GSD currently assesses a premium against any member...

Perspectives on Gender and Sexual Diversity (GSD)-Inclusive Education: Comparisons between Gay/Lesbian/Bisexual and Straight Educators

ERIC Educational Resources Information Center

Meyer, Elizabeth J.; Taylor, Catherine; Peter, Tracey

2015-01-01

This paper presents findings from a national study on the beliefs and practices of K-12 educators regarding lesbian, gay, bisexual, transgender, and queer (LGBTQ) issues in schools. Over 3400 Canadian educators participated in the study, which took the form of a bilingual (English/French) online survey. Respondents answered questions about their…

GSD Update: All together now: Collaboration in research and stewardship for our 21st century lands

Treesearch

Lisa-Natalie Anjozian

2012-01-01

Collaboration is the way the USDA Forest Service operates in this new era, where the challenges in our natural world - to soil, air, plants, animals, watersheds - require the talents of devoted scientists, managers, citizens, communities. Conservation of terrestrial and aquatic ecosystems depends on the success of these networks of involved participants in finding...

78 FR 35334 - Self-Regulatory Organizations; The Fixed Income Clearing Corporation; Order Granting Approval of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-12

...'') each use the Board of Governors of the Federal Reserve's (``FRB'') National Settlement Service (``NSS... NSS service by FICC. Pursuant to the GSD and MBSD rules, if FICC receives an FRB indemnity claim, FICC... which FICC should allocate an indemnity claim made in connection with the use of the FRB's NSS. The MBSD...

77 FR 40398 - Self-Regulatory Organizations; Fixed Income Clearing Corporation; Order Approving Proposed Rule...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-09

... prices of financial products that have maturity dates in the future as part of the volatility model in... Volatility Model in Its Clearing Fund Formula July 2, 2012. I. Introduction On May 15, 2012, the Fixed Income... simulation model currently used to calculate the VaR Charge in GSD's Clearing Fund formula is driven by...

77 FR 32153 - Self-Regulatory Organizations; Fixed Income Clearing Corporation; Notice of Filing of Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-31

... have maturity dates in the future as part of the volatility model in its Clearing Fund formula. \\16\\ 15... the Volatility Model in Its Clearing Fund Formula May 24, 2012. Pursuant to Section 19(b)(1) of the... ability of FICC GSD to use implied volatility indicators as part of the volatility model in its clearing...

The bioconcentration factor of perfluorooctane sulfonate is significantly larger than that of perfluorooctanoate in wild turtles (Trachemys scripta elegans and Chinemys reevesii): an Ai river ecological study in Japan.

PubMed

Morikawa, Akiko; Kamei, Naoya; Harada, Kouji; Inoue, Kayoko; Yoshinaga, Takeo; Saito, Norimitsu; Koizumi, Akio

2006-09-01

Turtles rank high in the river food chain, and are suitable for predicting the bioconcentrations of chemicals through the food chain. Trachemys scripta elegans (N=46) and Chinemys reevesii (N=51) were captured in a river in Japan, from September to October 2003 and April to June 2004. Surface water samples were collected simultaneously from the same sites at which the turtles were caught. Serum perfluorooctane sulfonate (PFOS) ranged from 2.4 to 486 microg/L, while water PFOS levels ranged from 2.9 to 37 ng/L. The geometric mean (GM) (geometric standard deviation, GSD) of the bioconcentration factor (BCF) of PFOS was 10,964 (2.5). In contrast, the perfluorooctanoate (PFOA) level in water ranged from 16.7-87,100 ng/L, and serum PFOA ranged from <0.2 to 870 microg/L. The GM (GSD) of the BCF of PFOA was 3.2 (7.9). Furthermore, the BCF of PFOA decreased as the PFOA level in the surface water increased. PFOS could be preferentially bioconcentrated in biota, and PFOA, slightly bioconcentrated.

Dropout From an eHealth Intervention for Adults With Type 2 Diabetes: A Qualitative Study

PubMed Central

Karlsen, Bjørg; Oord, Ellen Renate; Graue, Marit; Oftedal, Bjørg

2017-01-01

Background Adequate self-management is the cornerstone of type 2 diabetes treatment, as people make the majority of daily treatment measures and health decisions. The increasing prevalence of type 2 diabetes mellitus (T2DM) and the complexity of diabetes self-management demonstrate the need for innovative and effective ways to deliver self-management support. eHealth interventions are promoted worldwide and hold a great potential in future health care for people with chronic diseases such as T2DM. However, many eHealth interventions face high dropout rates. This led to our interest in the experiences of participants who dropped out of an eHealth intervention for adults with T2DM, based on the Guided Self-Determination (GSD) counseling method. Objective In this study, we aimed to explore experiences with an eHealth intervention based on GSD in general practice from the perspective of those who dropped out and to understand their reasons for dropping out. To the best of our knowledge, no previous qualitative study has focused on participants who withdrew from an eHealth self-management support intervention for adults with T2DM. Methods A qualitative design based on telephone interviews was used to collect data. The sample comprised 12 adults with type 2 diabetes who dropped out of an eHealth intervention. Data were collected in 2016 and subjected to qualitative content analysis. Results We identified one overall theme: “Losing motivation for intervention participation.” This theme was illustrated by four categories related to the participants’ experiences of the eHealth intervention: (1) frustrating technology, (2) perceiving the content as irrelevant and incomprehensible, (3) choosing other activities and perspectives, and (4) lacking face-to-face encounters. Conclusions Our findings indicate that the eHealth intervention based on GSD without face-to-face encounters with nurses reduced participants’ motivation for engagement in the intervention. To maintain motivation, our study points to the importance of combining eHealth with regular face-to-face consultations. Our study also shows that the perceived benefit of the GSD eHealth intervention intertwined with choosing to focus on other matters in complex daily lives are critical aspects in motivation for such interventions. This indicates the importance of giving potential participants tailored information about the aim, the content, and the effort needed to remain engaged in complex interventions so that eligible participants are recruited. Finally, motivation for engagement in the eHealth intervention was influenced by the technology used in this study. It seems important to facilitate more user-friendly but high-security eHealth technology. Our findings have implications for improving the eHealth intervention and to inform researchers and health care providers who are organizing eHealth interventions focusing on self-management support in order to reduce dropout rates. PMID:28559223

Dropout From an eHealth Intervention for Adults With Type 2 Diabetes: A Qualitative Study.

PubMed

Lie, Silje Stangeland; Karlsen, Bjørg; Oord, Ellen Renate; Graue, Marit; Oftedal, Bjørg

2017-05-30

Adequate self-management is the cornerstone of type 2 diabetes treatment, as people make the majority of daily treatment measures and health decisions. The increasing prevalence of type 2 diabetes mellitus (T2DM) and the complexity of diabetes self-management demonstrate the need for innovative and effective ways to deliver self-management support. eHealth interventions are promoted worldwide and hold a great potential in future health care for people with chronic diseases such as T2DM. However, many eHealth interventions face high dropout rates. This led to our interest in the experiences of participants who dropped out of an eHealth intervention for adults with T2DM, based on the Guided Self-Determination (GSD) counseling method. In this study, we aimed to explore experiences with an eHealth intervention based on GSD in general practice from the perspective of those who dropped out and to understand their reasons for dropping out. To the best of our knowledge, no previous qualitative study has focused on participants who withdrew from an eHealth self-management support intervention for adults with T2DM. A qualitative design based on telephone interviews was used to collect data. The sample comprised 12 adults with type 2 diabetes who dropped out of an eHealth intervention. Data were collected in 2016 and subjected to qualitative content analysis. We identified one overall theme: "Losing motivation for intervention participation." This theme was illustrated by four categories related to the participants' experiences of the eHealth intervention: (1) frustrating technology, (2) perceiving the content as irrelevant and incomprehensible, (3) choosing other activities and perspectives, and (4) lacking face-to-face encounters. Our findings indicate that the eHealth intervention based on GSD without face-to-face encounters with nurses reduced participants' motivation for engagement in the intervention. To maintain motivation, our study points to the importance of combining eHealth with regular face-to-face consultations. Our study also shows that the perceived benefit of the GSD eHealth intervention intertwined with choosing to focus on other matters in complex daily lives are critical aspects in motivation for such interventions. This indicates the importance of giving potential participants tailored information about the aim, the content, and the effort needed to remain engaged in complex interventions so that eligible participants are recruited. Finally, motivation for engagement in the eHealth intervention was influenced by the technology used in this study. It seems important to facilitate more user-friendly but high-security eHealth technology. Our findings have implications for improving the eHealth intervention and to inform researchers and health care providers who are organizing eHealth interventions focusing on self-management support in order to reduce dropout rates. ©Silje Stangeland Lie, Bjørg Karlsen, Ellen Renate Oord, Marit Graue, Bjørg Oftedal. Originally published in the Journal of Medical Internet Research (http://www.jmir.org), 30.05.2017.

The Chinese People’s Militia and the Doctrine of People’s War,

DTIC Science & Technology

1983-01-01

matter of PRC military modernization and related defense issues. The National De- fense University is pleased to present this work for discussion in the...specific functional area of GSD work . The General Logistics Department (GLD) is responsible for centralized logistics planning, procurement, and...Exercising leadership over political security work and supervising military procuratorates and courts. * Publishing PLA newspapers and other publications

GSD Update: It works both ways: How scientists and managers join forces to conserve today’s natural resources

Treesearch

Karen Bagne; Deborah Finch

2012-01-01

Helping managers to promote sustainability on the Nation's forests and grasslands has been an overarching goal of US Forest Service Research and Development from the agency's beginnings in 1905. Major advances in resource management have been made with the help of science, from increasing yields of commodities like timber and cattle to providing services such...

Strategies for the Interpretive Integration of Ground and Aerial Views in UGV Operations

DTIC Science & Technology

2006-11-01

conjoinment of the psychological processes and effects of perception, object recognition (i.e. Biederman & Gerhardstein, 1993), navigation (Wickens...rather simple geon structural descriptions (GSD, see Biederman & 4 Gerhardstein, 1993). A geon is a basic three dimensional solid shape, such as a...large and reliable sex difference. Behavioral Brain Research, 93, 185-190. Biederman , I., & Gerhardstein, C. (1993). Recognizing depth- rotated objects

Occupational health concerns in the welding industry.

PubMed

Korczynski, R E

2000-12-01

The Workplace Safety and Health Branch initiated a proactive study in the welding industry in Manitoba. Eight welding companies participated in this study. Health concerns raised by welders were welders' flash, sore/red/teary eyes, headaches, nosebleeds, and a black mucous discharge from their nasal membrane. Most welders expressed concern regarding excessive smoke levels in the workplace and inadequate ventilation. Types of welding identified were MIG mild steel, MIG stainless steel, and TIG aluminum. Monitoring involved an assessment of noise levels, fume composition, and carbon monoxide and ozone concentrations. Metal analyses were according to National Institute for Occupational Safety and Health (NIOSH) Method 7300. Noise dosimeters used were the Quest model 100 and Micro 14 & 15. Carbon monoxide was monitored using the Gastech Model 4700 and ozone using the AID Portable Ozone Meter Model 560. In Manitoba, a hearing conservation program is required when the equivalent sound exposure level (normalized Lex 8-hr) exceeds 80 dBA-weighted. The American Conference of Governmental Industrial Hygienists' threshold limit value-time weighted average (ACGIH TLV-TWA) for iron is 5.0 mg/m3, manganese is 0.2 mg/m3, carbon monoxide is 25 ppm, and ozone is 0.05 ppm (heavy work), 0.08 ppm (moderate work), and 0.1 ppm (light work). Welders' personal exposures to manganese ranged from 0.01-4.93 mg/m3 (N = 42; AM = 0.5; GM = 0.2; SD +/- 0.9; GSD +/- 3.2) and to iron ranged from 0.04-16.29 mg/m3 (N = 42; AM = 3.0; GM = 1.4; SD +/- 3.5; GSD +/- 2.5). Noise exposures ranged from 79-98 dBA (N = 44; AM = 88.9; GM = 88.8; SD +/- 4.2; GSD +/- 1.0). Carbon monoxide levels were less than 5.0 ppm (at source) and ozone levels varied from 0.4-0.6 ppm (at source). Ventilation upgrades in the workplace were required in most welding shops. Only 7 percent of the welders wore respiratory protection. A hearing conservation program and hearing protection were required at all monitored workplaces.

Determinants of dust exposure in tunnel construction work.

PubMed

Bakke, Berit; Stewart, Patricia; Eduard, Wijnand

2002-11-01

In tunnel construction work, dust is generated from rock drilling, rock bolting, grinding, scaling, and transport operations. Other important dust-generating activities are blasting rock and spraying wet concrete on tunnel walls for strength and finishing work. The aim of this study was to identify determinants of dust exposure in tunnel construction work and to propose control measures. Personal exposures to total dust, respirable dust, and alpha-quartz were measured among 209 construction workers who were divided into 8 job groups performing similar tasks: drill and blast workers, shaft drilling workers, tunnel boring machine workers, shotcreting operators, support workers, concrete workers, outdoor concrete workers, and electricians. Information on determinants was obtained from interviewing the workers, observation by the industrial hygienist responsible for the sampling, and the job site superintendent. Multivariate regression models were used to identify determinants associated with the dust exposures within the job groups. The geometric mean exposure to total dust, respirable dust, and alpha-quartz for all tunnel workers was 3.5 mg/m(3) (GSD = 2.6), 1.2 mg/m(3) (GSD = 2.4), and 0.035 mg/m(3) (GSD = 5.0), respectively. A total of 15 percent of the total dust measurements, 5 percent of the respirable dust, and 21 percent of the alpha-quartz exceeded the Norwegian OELs of 10 mg/m(3), 5 mg/m(3), and 0.1 mg/m(3), respectively. Job groups with highest geometric mean total dust exposure were shotcreting operators (6.8 mg/m(3)), tunnel boring machine workers (6.2 mg/m(3)), and shaft drilling workers (6.1 mg/m(3)). The lowest exposed groups to total dust were outdoor concrete workers (1.0 mg/m(3)), electricians (1.4 mg/m(3)), and support workers (1.9 mg/m(3)). Important determinants of exposure were job group, job site, certain tasks (e.g., drilling and scaling), the presence of a cab, and breakthrough of the tunnel. The use of ventilated, closed cabs appeared to be the single most important control measure for lowering exposures.

Exploring Manganese Fractionation Using a Sequential Extraction Method to Evaluate Welders' Gas Metal Arc Welding Exposures during Heavy Equipment Manufacturing.

PubMed

Hanley, Kevin W; Andrews, Ronnee; Bertke, Steven; Ashley, Kevin

2017-01-01

The National Institute for Occupational Safety and Health (NIOSH) has conducted an occupational exposure assessment study of manganese (Mn) in welding fume at three factories where heavy equipment was manufactured. The objective of this study was to evaluate exposures to different Mn fractions using a sequential extraction procedure. One hundred nine worker-days were monitored for either total or respirable Mn during gas metal arc welding. The samples were analyzed using an experimental method to separate different Mn fractions based on selective chemical solubility. The full-shift total particle size Mn time-weighted average (TWA) breathing zone concentrations ranged 0.38-26 for soluble Mn in a mild ammonium acetate solution; 3.2-170 for Mn0,2+ in acetic acid; 3.1-290 for Mn3+,4+ in hydroxylamine-hydrochloride; and non-detectable (ND)-130 µg m-3 for insoluble Mn fractions in hydrochloric and nitric acid. The summation of all the total particulate Mn TWA fractions yielded results that ranged from 6.9 to 610 µg m-3. The range of respirable size Mn TWA concentrations were 0.33-21 for soluble Mn; 15-140 for Mn0,2+; 14-170 for Mn3+,4+; 5.3-230 for insoluble Mn; and 36-530 µg m-3 for Mn (sum of fractions). Total particulate TWA GM concentrations of the Mn (sum) were 53 (GSD = 2.5), 150 (GSD = 1.7), and 120 (GSD = 1.8) µg m-3 for the three separate factories. Although all of the workers' exposures were measured below the OSHA regulatory permissible exposure limit and NIOSH recommended exposure limit for Mn, 70 welders' exposures exceeded the ACGIH Threshold Limit Values® for total Mn (100 µg m-3) and 29 exceeded the recently adopted respirable Mn TLV (20 µg m-3). This study shows that a welding fume exposure control and management program is warranted for Mn, which includes improved exhaust ventilation and may necessitate the use of respiratory protection, especially for welding parts that impede air circulation. Published by Oxford University Press on behalf of the British Occupational Hygiene Society 2017.

Considering Time in Orthophotography Production: from a General Workflow to a Shortened Workflow for a Faster Disaster Response

NASA Astrophysics Data System (ADS)

Lucas, G.

2015-08-01

This article overall deals with production time with orthophoto imagery with medium size digital frame camera. The workflow examination follows two main parts: data acquisition and post-processing. The objectives of the research are fourfold: 1/ gathering time references for the most important steps of orthophoto production (it turned out that literature is missing on this topic); these figures are used later for total production time estimation; 2/ identifying levers for reducing orthophoto production time; 3/ building a simplified production workflow for emergency response: less exigent with accuracy and faster; and compare it to a classical workflow; 4/ providing methodical elements for the estimation of production time with a custom project. In the data acquisition part a comprehensive review lists and describes all the factors that may affect the acquisition efficiency. Using a simulation with different variables (average line length, time of the turns, flight speed) their effect on acquisition efficiency is quantitatively examined. Regarding post-processing, the time references figures were collected from the processing of a 1000 frames case study with 15 cm GSD covering a rectangular area of 447 km2; the time required to achieve each step during the production is written down. When several technical options are possible, each one is tested and time documented so as all alternatives are available. Based on a technical choice with the workflow and using the compiled time reference of the elementary steps, a total time is calculated for the post-processing of the 1000 frames. Two scenarios are compared as regards to time and accuracy. The first one follows the "normal" practices, comprising triangulation, orthorectification and advanced mosaicking methods (feature detection, seam line editing and seam applicator); the second is simplified and make compromise over positional accuracy (using direct geo-referencing) and seamlines preparation in order to achieve orthophoto production faster. The shortened workflow reduces the production time by more than three whereas the positional error increases from 1 GSD to 1.5 GSD. The examination of time allocation through the production process shows that it is worth sparing time in the post-processing phase.

Photogrammetric Processing of IceBridge DMS Imagery into High-Resolution Digital Surface Models (DEM and Visible Overlay)

NASA Astrophysics Data System (ADS)

Arvesen, J. C.; Dotson, R. C.

2014-12-01

The DMS (Digital Mapping System) has been a sensor component of all DC-8 and P-3 IceBridge flights since 2009 and has acquired over 3 million JPEG images over Arctic and Antarctic land and sea ice. The DMS imagery is primarily used for identifying and locating open leads for LiDAR sea-ice freeboard measurements and documenting snow and ice surface conditions. The DMS is a COTS Canon SLR camera utilizing a 28mm focal length lens, resulting in a 10cm GSD and swath of ~400 meters from a nominal flight altitude of 500 meters. Exterior orientation is provided by an Applanix IMU/GPS which records a TTL pulse coincident with image acquisition. Notable for virtually all IceBridge flights is that parallel grids are not flown and thus there is no ability to photogrammetrically tie any imagery to adjacent flight lines. Approximately 800,000 Level-3 DMS Surface Model data products have been delivered to NSIDC, each consisting of a Digital Elevation Model (GeoTIFF DEM) and a co-registered Visible Overlay (GeoJPEG). Absolute elevation accuracy for each individual Elevation Model is adjusted to concurrent Airborne Topographic Mapper (ATM) Lidar data, resulting in higher elevation accuracy than can be achieved by photogrammetry alone. The adjustment methodology forces a zero mean difference to the corresponding ATM point cloud integrated over each DMS frame. Statistics are calculated for each DMS Elevation Model frame and show RMS differences are within +/- 10 cm with respect to the ATM point cloud. The DMS Surface Model possesses similar elevation accuracy to the ATM point cloud, but with the following advantages: · Higher and uniform spatial resolution: 40 cm GSD · 45% wider swath: 435 meters vs. 300 meters at 500 meter flight altitude · Visible RGB co-registered overlay at 10 cm GSD · Enhanced visualization through 3-dimensional virtual reality (i.e. video fly-through) Examples will be presented of the utility of these advantages and a novel use of a cell phone camera for aerial photogrammetry will also be presented.

Lead gunshot pellet ingestion and tissue lead levels in wild ducks from Argentine hunting hotspots.

PubMed

Ferreyra, Hebe; Romano, Marcelo; Beldomenico, Pablo; Caselli, Andrea; Correa, Ana; Uhart, Marcela

2014-05-01

Lead poisoning in waterfowl due to ingestion of lead pellets is a long recognized worldwide problem but poorly studied in South America, particularly in Argentinean wetlands where duck hunting with lead gunshot is extensive. In 2008, we found high pellet ingestion rates in a small sample of hunted ducks. To expand our knowledge on the extent of lead exposure and to assess health risks from spent shot intake, during 2011 and 2012 we sampled 415 hunter-killed ducks and 96 live-trapped ducks. We determined the incidence of lead shot ingestion and lead concentrations in bone, liver and blood in five duck species: whistling duck (Dendrocygna bicolor), white-faced tree duck (D. viduata), black-bellied whistling-duck (D. autumnalis), rosy-billed pochard (Netta peposaca) and Brazilian duck (Amazonetta brasiliensis). The ingestion of lead shot was confirmed in 10.4% of the ducks examined (43/415), with a prevalence that varied by site and year, from 7.6% to 50%. All bone samples (n=382) and over 60% of liver samples (249/412) contained lead concentrations above the detection limit. The geometric mean lead concentration in tissues (mg/kg dry weight) was 0.31 (GSD=3.93) and 3.61 (GSD=4.02) for liver and bone, respectively, and 0.20 (GSD=2.55) in blood (mg/kg wet weight). Lead levels surpassed toxicity thresholds at which clinical poisoning is expected in 3.15% of liver samples, 23.8% of bones and 28% of blood samples. Ducks with ingested lead pellets were much more likely to have high levels of lead in their liver. Rosy-billed pochards were consistently more prone to ingesting lead shot than other duck species sampled. However, whistling ducks showed higher levels of lead in liver and bone. Our results suggest that lead from ammunition could become a substantial threat for the conservation of wild duck populations in Argentina. The replacement of lead by non-toxic shot would be a reasonable and effective solution to this problem. © 2013 Published by Elsevier Inc.

Performance of a scanning mobility particle sizer in measuring diverse types of airborne nanoparticles: Multi-walled carbon nanotubes, welding fumes, and titanium dioxide spray.

PubMed

Chen, Bean T; Schwegler-Berry, Diane; Cumpston, Amy; Cumpston, Jared; Friend, Sherri; Stone, Samuel; Keane, Michael

2016-07-01

Direct-reading instruments have been widely used for characterizing airborne nanoparticles in inhalation toxicology and industrial hygiene studies for exposure/risk assessments. Instruments using electrical mobility sizing followed by optical counting, e.g., scanning or sequential mobility particle spectrometers (SMPS), have been considered as the "gold standard" for characterizing nanoparticles. An SMPS has the advantage of rapid response and has been widely used, but there is little information on its performance in assessing the full spectrum of nanoparticles encountered in the workplace. In this study, an SMPS was evaluated for its effectiveness in producing "monodisperse" aerosol and its adequacy in characterizing overall particle size distribution using three test aerosols, each mimicking a unique class of real-life nanoparticles: singlets of nearly spherical titanium dioxide (TiO2), agglomerates of fiber-like multi-walled carbon nanotube (MWCNT), and aggregates that constitutes welding fume (WF). These aerosols were analyzed by SMPS, cascade impactor, and by counting and sizing of discrete particles by scanning and transmission electron microscopy. The effectiveness of the SMPS to produce classified particles (fixed voltage mode) was assessed by examination of the resulting geometric standard deviation (GSD) from the impactor measurement. Results indicated that SMPS performed reasonably well for TiO2 (GSD = 1.3), but not for MWCNT and WF as evidenced by the large GSD values of 1.8 and 1.5, respectively. For overall characterization, results from SMPS (scanning voltage mode) exhibited particle-dependent discrepancies in the size distribution and total number concentration compared to those from microscopic analysis. Further investigation showed that use of a single-stage impactor at the SMPS inlet could distort the size distribution and underestimate the concentration as shown by the SMPS, whereas the presence of vapor molecules or atom clusters in some test aerosols might cause artifacts by counting "phantom particles." Overall, the information obtained from this study will help understand the limitations of the SMPS in measuring nanoparticles so that one can adequately interpret the results for risk assessments and exposure prevention in an occupational or ambient environment.

Modeling the Effect of Geomorphic Change Triggered by Large Wood Addition on Salmon Habitat in a Forested Coastal Watershed

NASA Astrophysics Data System (ADS)

Bair, R.; Segura, C.; Lorion, C.

2015-12-01

Large wood (LW) additions are often part of fish habitat restorations in the PNW where historic forest clear-cutting limited natural wood recruitment. These efforts' relative successes are rarely reported in terms of ecological significance to different life stages of fish. Understanding the effectiveness of LW additions will contribute to successfully managing forest land. In this study we quantify the geomorphic change of a restoration project involving LW additions to three alluvial reaches in Mill Creek, OR. The reaches are 110-130m in plane-bed morphology and drain 2-16km2. We quantify the change in available habitat to different life stages of coho salmon in terms of velocity (v), shear stress (t), flow depth, and grain size distributions (GSD) considering existing thresholds in the literature for acceptable habitat. Flow conditions before and after LW additions are assessed using a 2D hydrodynamic model (FaSTMECH). Model inputs include detailed channel topography, discharge, and surface GSD. The spatial-temporal variability of sediment transport was also quantified based the modeled t distributions and the GSD to document changes in the overall geomorphic regime. Initial modeling results for pre wood conditions show mean t and v values ranging between 0 and 26N/m2 and between 0 and 2.4m/s, respectively for up to bankfull flow (Qbf). The distributions of both t and v become progressively wider and peak at higher values as flow increases with the notable exception at Qbf for which the area of low velocity increases noticeably. The spatial distributions of velocity results indicates that the extent of suitable habitat for adult coho decreased by 18% between flows 30 and 55% of BF. However the area of suitable habitat increased by 15% between 0.55Qbf and Qbf as the flow spreads from the channel into the floodplain. We expect the LW will enhance floodplain connectivity and thus available habitat by creating additional areas of low v during winter flows.

The design and assembly of aluminum mirrors of a three-mirror-anastigmat telescope

NASA Astrophysics Data System (ADS)

Chang, Shenq-Tsong; Lin, Yu-Chuan; Wu, Kun-Huan; Lien, Chun-Chieh; Huang, Ting-Ming; Tsay, Ho-Lin; Chan, Chia-Yen

2017-09-01

Better ground sampling distance (GSD) has been a trend for earth observation satellites. A long-focal-length telescope is required accordingly in systematic point of view. On the other hand, there is size constraint for such long-focal-length telescope especially in space projects. Three-mirror-anastigmat (TMA) was proven to have excellent features of correcting aberrations, wide spectral range and shorter physical requirement [1-3].

Techniques for super-resolution microscopy using NV-diamond

NASA Astrophysics Data System (ADS)

Trifonov, Alexei; Glenn, David; Bar-Gill, Nir; Le Sage, David; Walsworth, Ronald

2011-05-01

We discuss the development and application of techniques for super-resolution microscopy using NV centers in diamond: stimulated emission depletion (STED), metastable ground state depletion (GSD), and stochastic optical reconstruction microscopy (STORM). NV centers do not bleach under optical excitation, are not biotoxic, and have long-lived electronic spin coherence and spin-state-dependent fluorescence. Thus NV-diamond has great potential as a fluorescent biomarker and as a magnetic biosensor.

GSD Update: What are invasive species? ... And do we really need to worry about them?

Treesearch

Catherine Dold

2011-01-01

Invasive species are the focus of the September 2011 issue of GSDUpdate: What Are Invasive Species? And Do We Really Need to Worry About Them? An invasive species is any species - non-native or native to a region - that could cause economic or ecological harm to an area. Invasives can be weeds, shrubs and trees, insects, mollusks, vertebrates and even microorganisms...

A novel homozygous no-stop mutation in G6PC gene from a Chinese patient with glycogen storage disease type Ia.

PubMed

Gu, Lei-Lei; Li, Xin-Hua; Han, Yue; Zhang, Dong-Hua; Gong, Qi-Ming; Zhang, Xin-Xin

2014-02-25

Glycogen storage disease type Ia (GSD-Ia) is an autosomal recessive genetic disorder resulting in hypoglycemia, hepatomegaly and growth retardation. It is caused by mutations in the G6PC gene encoding Glucose-6-phosphatase. To date, over 80 mutations have been identified in the G6PC gene. Here we reported a novel mutation found in a Chinese patient with abnormal transaminases, hypoglycemia, hepatomegaly and short stature. Direct sequencing of the coding region and splicing-sites in the G6PC gene revealed a novel no-stop mutation, p.*358Yext*43, leading to a 43 amino-acid extension of G6Pase. The expression level of mutant G6Pase transcripts was only 7.8% relative to wild-type transcripts. This mutation was not found in 120 chromosomes from 60 unrelated healthy control subjects using direct sequencing, and was further confirmed by digestion with Rsa I restriction endonuclease. In conclusion, we revealed a novel no-stop mutation in this study which expands the spectrum of mutations in the G6PC gene. The molecular genetic analysis was indispensable to the diagnosis of GSD-Ia for the patient. Copyright © 2013 Elsevier B.V. All rights reserved.

Study of G-S/D underlap for enhanced analog performance and RF/circuit analysis of UTB InAs-OI-Si MOSFET using NQS small signal model

NASA Astrophysics Data System (ADS)

Maity, Subir Kumar; Pandit, Soumya

2017-01-01

InGaAs (and its variant) appears to be a promising channel material for high-performance, low-power scaled CMOS applications due to its excellent carrier transport properties. However, MOS transistors made of this suffer from poor electrostatic integrity. In this work, we consider an underlap ultra thin body (UTB) InAs-on-Insulator n-channel MOS transistor, and study the effect of varying the gate-source/drain (G-S/D) underlap length on the analog performance of the device with the help of technology computer-aided design (TCAD) simulation, calibrated with Schrodinger-Poisson solver and experimental results. The underlap technique improves the gate electrostatic integrity which in turn improves the analog performance. We develop a non-quasi-static (NQS) small signal equivalent circuit model of the device which is used for study of the RF performance. With increase of the underlap length, the unity gain cut-off frequency degrades and the maximum oscillation frequency improves beyond a certain value of the underlap length. We further study the gain-frequency response of a common source amplifier using the NQS model, through SPICE simulation and observe that the voltage gain and the gain bandwidth improves.

An extension of the Saltykov method to quantify 3D grain size distributions in mylonites

NASA Astrophysics Data System (ADS)

Lopez-Sanchez, Marco A.; Llana-Fúnez, Sergio

2016-12-01

The estimation of 3D grain size distributions (GSDs) in mylonites is key to understanding the rheological properties of crystalline aggregates and to constraining dynamic recrystallization models. This paper investigates whether a common stereological method, the Saltykov method, is appropriate for the study of GSDs in mylonites. In addition, we present a new stereological method, named the two-step method, which estimates a lognormal probability density function describing the 3D GSD. Both methods are tested for reproducibility and accuracy using natural and synthetic data sets. The main conclusion is that both methods are accurate and simple enough to be systematically used in recrystallized aggregates with near-equant grains. The Saltykov method is particularly suitable for estimating the volume percentage of particular grain-size fractions with an absolute uncertainty of ±5 in the estimates. The two-step method is suitable for quantifying the shape of the actual 3D GSD in recrystallized rocks using a single value, the multiplicative standard deviation (MSD) parameter, and providing a precision in the estimate typically better than 5%. The novel method provides a MSD value in recrystallized quartz that differs from previous estimates based on apparent 2D GSDs, highlighting the inconvenience of using apparent GSDs for such tasks.

Image Stability Requirements For a Geostationary Imaging Fourier Transform Spectrometer (GIFTS)

NASA Technical Reports Server (NTRS)

Bingham, G. E.; Cantwell, G.; Robinson, R. C.; Revercomb, H. E.; Smith, W. L.

2001-01-01

A Geostationary Imaging Fourier Transform Spectrometer (GIFTS) has been selected for the NASA New Millennium Program (NMP) Earth Observing-3 (EO-3) mission. Our paper will discuss one of the key GIFTS measurement requirements, Field of View (FOV) stability, and its impact on required system performance. The GIFTS NMP mission is designed to demonstrate new and emerging sensor and data processing technologies with the goal of making revolutionary improvements in meteorological observational capability and forecasting accuracy. The GIFTS payload is a versatile imaging FTS with programmable spectral resolution and spatial scene selection that allows radiometric accuracy and atmospheric sounding precision to be traded in near real time for area coverage. The GIFTS sensor combines high sensitivity with a massively parallel spatial data collection scheme to allow high spatial resolution measurement of the Earth's atmosphere and rapid broad area coverage. An objective of the GIFTS mission is to demonstrate the advantages of high spatial resolution (4 km ground sample distance - gsd) on temperature and water vapor retrieval by allowing sampling in broken cloud regions. This small gsd, combined with the relatively long scan time required (approximately 10 s) to collect high resolution spectra from geostationary (GEO) orbit, may require extremely good pointing control. This paper discusses the analysis of this requirement.

Echocardiographic Manifestations of Glycogen Storage Disease III: Increase in Wall Thickness and Left Ventricular Mass over Time

PubMed Central

Vertilus, Shawyntee M.; Austin, Stephanie L.; Foster, Kimberly S.; Boyette, Keri E.; Bali, Deeksha; Li, Jennifer S.; Kishnani, Priya S.; Wechsler, Stephanie Burns

2013-01-01

Purpose Glycogen Storage Disease (GSD) type III, glycogen debranching enzyme deficiency, causes accumulation of glycogen in liver, skeletal, and cardiac muscle. Some patients develop increased left ventricular (LV) thickness by echocardiography, but the rate of increase and its significance remain unclear. Methods We evaluated 33 patients with GSD type III, 23 with IIIa and 10 with IIIb, ages 1 month – 55.5 yrs, by echocardiography for wall thickness, LV mass, shortening and ejection fractions, at 1 time point (n = 33) and at 2 time points in patients with more than 1 echocardiogram (13 of the 33). Results Of 23 cross-sectional patients with type IIIa, 12 had elevated LV mass, 11 had elevated wall thickness. One type IIIb patient had elevated LV mass but 4 had elevated wall thickness. For those with multiple observations, 9 of 10 with type IIIa developed increased LV mass over time, with 3 already increased at first measurement. Shortening and ejection fractions were generally normal. Conclusion Elevated LV mass and wall thickness is more common in patients with type IIIa but develops rarely in type IIIb, though ventricular systolic function is preserved. This suggests serial echocardiograms with attention to LV thickness and mass are important for care of these patients. PMID:20526204

Global Software Development with Cloud Platforms

NASA Astrophysics Data System (ADS)

Yara, Pavan; Ramachandran, Ramaseshan; Balasubramanian, Gayathri; Muthuswamy, Karthik; Chandrasekar, Divya

Offshore and outsourced distributed software development models and processes are facing challenges, previously unknown, with respect to computing capacity, bandwidth, storage, security, complexity, reliability, and business uncertainty. Clouds promise to address these challenges by adopting recent advances in virtualization, parallel and distributed systems, utility computing, and software services. In this paper, we envision a cloud-based platform that addresses some of these core problems. We outline a generic cloud architecture, its design and our first implementation results for three cloud forms - a compute cloud, a storage cloud and a cloud-based software service- in the context of global distributed software development (GSD). Our ”compute cloud” provides computational services such as continuous code integration and a compile server farm, ”storage cloud” offers storage (block or file-based) services with an on-line virtual storage service, whereas the on-line virtual labs represent a useful cloud service. We note some of the use cases for clouds in GSD, the lessons learned with our prototypes and identify challenges that must be conquered before realizing the full business benefits. We believe that in the future, software practitioners will focus more on these cloud computing platforms and see clouds as a means to supporting a ecosystem of clients, developers and other key stakeholders.

Depletion-based techniques for super-resolution imaging of NV-diamond

NASA Astrophysics Data System (ADS)

Jaskula, Jean-Christophe; Trifonov, Alexei; Glenn, David; Walsworth, Ronald

2012-06-01

We discuss the development and application of depletion-based techniques for super-resolution imaging of NV centers in diamond: stimulated emission depletion (STED), metastable ground state depletion (GSD), and dark state depletion (DSD). NV centers in diamond do not bleach under optical excitation, are not biotoxic, and have long-lived electronic spin coherence and spin-state-dependent fluorescence. Thus NV-diamond has great potential as a fluorescent biomarker and as a magnetic biosensor.

Using Scrum Practices in GSD Projects

NASA Astrophysics Data System (ADS)

Paasivaara, Maria; Lassenius, Casper

In this chapter we present advice for applying Scrum practices to globally distributed software development projects. The chapter is based on a multiple-case study of four distributed Scrum projects. We discuss the use of distributed daily Scrums, Scrum-of-Scrums, Sprints, Sprint planning meetings, Sprint Demos, Retrospective meetings, and Backlogs. Moreover, we present lessons that distributed Scrum projects can benefit from non-agile globally distributed software development projects: frequent visits and multiple communication modes.

Comparison of Methods for Analyzing Left-Censored Occupational Exposure Data

PubMed Central

Huynh, Tran; Ramachandran, Gurumurthy; Banerjee, Sudipto; Monteiro, Joao; Stenzel, Mark; Sandler, Dale P.; Engel, Lawrence S.; Kwok, Richard K.; Blair, Aaron; Stewart, Patricia A.

2014-01-01

The National Institute for Environmental Health Sciences (NIEHS) is conducting an epidemiologic study (GuLF STUDY) to investigate the health of the workers and volunteers who participated from April to December of 2010 in the response and cleanup of the oil release after the Deepwater Horizon explosion in the Gulf of Mexico. The exposure assessment component of the study involves analyzing thousands of personal monitoring measurements that were collected during this effort. A substantial portion of these data has values reported by the analytic laboratories to be below the limits of detection (LOD). A simulation study was conducted to evaluate three established methods for analyzing data with censored observations to estimate the arithmetic mean (AM), geometric mean (GM), geometric standard deviation (GSD), and the 95th percentile (X0.95) of the exposure distribution: the maximum likelihood (ML) estimation, the β-substitution, and the Kaplan–Meier (K-M) methods. Each method was challenged with computer-generated exposure datasets drawn from lognormal and mixed lognormal distributions with sample sizes (N) varying from 5 to 100, GSDs ranging from 2 to 5, and censoring levels ranging from 10 to 90%, with single and multiple LODs. Using relative bias and relative root mean squared error (rMSE) as the evaluation metrics, the β-substitution method generally performed as well or better than the ML and K-M methods in most simulated lognormal and mixed lognormal distribution conditions. The ML method was suitable for large sample sizes (N ≥ 30) up to 80% censoring for lognormal distributions with small variability (GSD = 2–3). The K-M method generally provided accurate estimates of the AM when the censoring was <50% for lognormal and mixed distributions. The accuracy and precision of all methods decreased under high variability (GSD = 4 and 5) and small to moderate sample sizes (N < 20) but the β-substitution was still the best of the three methods. When using the ML method, practitioners are cautioned to be aware of different ways of estimating the AM as they could lead to biased interpretation. A limitation of the β-substitution method is the absence of a confidence interval for the estimate. More research is needed to develop methods that could improve the estimation accuracy for small sample sizes and high percent censored data and also provide uncertainty intervals. PMID:25261453

Sex determination mode does not affect body or genital development of the central bearded dragon (Pogona vitticeps).

PubMed

Whiteley, Sarah L; Holleley, Clare E; Ruscoe, Wendy A; Castelli, Meghan; Whitehead, Darryl L; Lei, Juan; Georges, Arthur; Weisbecker, Vera

2017-01-01

The development of male- or female-specific phenotypes in squamates is typically controlled by either temperature-dependent sex determination (TSD) or chromosome-based genetic sex determination (GSD). However, while sex determination is a major switch in individual phenotypic development, it is unknownhow evolutionary transitions between GSD and TSD might impact on the evolution of squamate phenotypes, particularly the fast-evolving and diverse genitalia. Here, we take the unique opportunity of studying the impact of both sex determination mechanisms on the embryological development of the central bearded dragon ( Pogona vitticeps ). This is possible because of the transitional sex determination system of this species, in which genetically male individuals reverse sex at high incubation temperatures. This can trigger the evolutionary transition of GSD to TSD in a single generation, making P. vitticeps an ideal model organism for comparing the effects of both sex determination processes in the same species. We conducted four incubation experiments on 265 P. vitticeps eggs, covering two temperature regimes ("normal" at 28 °C and "sex reversing" at 36 °C) and the two maternal sexual genotypes (concordant ZW females or sex-reversed ZZ females). From this, we provide the first detailed staging system for the species, with a focus on genital and limb development. This was augmented by a new sex chromosome identification methodology for P. vitticeps that is non-destructive to the embryo. We found a strong correlation between embryo age and embryo stage. Aside from faster growth in 36 °C treatments, body and external genital development was entirely unperturbed by temperature, sex reversal or maternal sexual genotype. Unexpectedly, all females developed hemipenes (the genital phenotype of adult male P. vitticeps ), which regress close to hatching. The tight correlation between embryo age and embryo stage allows the precise targeting of specific developmental periods in the emerging field of molecular research on P. vitticeps . The stability of genital development in all treatments suggests that the two sex-determining mechanisms have little impact on genital evolution, despite their known role in triggering genital development. Hemipenis retention in developing female P. vitticeps , together with frequent occurrences of hemipenis-like structures during development in other squamate species, raises the possibility of a bias towards hemipenis formation in the ancestral developmental programme for squamate genitalia.

Persian Gulf: U.S. Agencies Need to Improve Licensing Data and to Document Reviews of Arms Transfers for U.S. Foreign Policy and National Security Goals

DTIC Science & Technology

2010-09-01

of Defense DTSA Defense Technology Security Administration FMS Foreign Military Sales GSD Gulf Security Dialogue LOA Letter of Offer and...may refer license applications to the Defense Technology Security Administration ( DTSA ) to determine if the United States should release the...requested technology to the requesting foreign government. Specifically, DTSA assesses the sale’s impact on preserving critical U.S. military technological

Unexpected resilience of species with temperature-dependent sex determination at the Cretaceous–Palaeogene boundary

PubMed Central

Silber, Sherman; Geisler, Jonathan H.; Bolortsetseg, Minjin

2011-01-01

It has been suggested that climate change at the Cretaceous–Palaeogene (K–Pg) boundary, initiated by a bolide impact or volcanic eruptions, caused species with temperature-dependent sex determination (TSD), including dinosaurs, to go extinct because of a skewed sex ratio towards all males. To test this hypothesis, the sex-determining mechanisms (SDMs) of Cretaceous tetrapods of the Hell Creek Formation (Montana, USA) were inferred using parsimony optimizations of SDMs on a tree, including Hell Creek species and their extant relatives. Although the SDMs of non-avian dinosaurs could not be inferred, we were able to determine the SDMs of 62 species; 46 had genotypic sex determination (GSD) and 16 had TSD. The TSD hypothesis for extinctions performed poorly, predicting between 32 and 34 per cent of survivals and extinctions. Most surprisingly, of the 16 species with TSD, 14 of them survived into the Early Palaeocene. In contrast, 61 per cent of species with GSD went extinct. Possible explanations include minimal climate change at the K–Pg, or if climate change did occur, TSD species that survived had egg-laying behaviour that prevented the skewing of sex ratios, or had a sex ratio skewed towards female rather than male preponderance. Application of molecular clocks may allow the SDMs of non-avian dinosaurs to be inferred, which would be an important test of the pattern discovered here. PMID:20980293

A case of disseminated mycosis in a German shepherd dog due to Penicillium purpurogenum.

PubMed

Zanatta, R; Miniscalco, B; Guarro, J; Gené, J; Capucchio, M T; Gallo, M G; Mikulicich, B; Peano, A

2006-02-01

The genus Penicillium is among the most common contaminant fungi in the environment. Around 15 species are known to cause opportunistic human mycoses, in immunocompromised patients. Until now, Penicillium purpurogenum has been involved in only three human cases of pulmonary diseases but no infections in animals have been reported. Most disseminated mycoses in dogs are caused by members of the genus Aspergillus, with the predisposing factors in these cases being difficult to define. The case reported here involved a 4-year-old female German shepherd dog (GSD) with forelimb instability and back pain. Clinical examination showed hyperthermia, generalized lymphadenomegaly and kyphosis. Radiological findings of the spine revealed areas of discospondilitis involving thoracic and lumbar vertebrae. Microscopic observations of fine needle aspiration biopsies (FNAB) of lymph-nodes showed regular, septate, branching fungal hyphae. Itraconazole therapy was started but the subject died six days later. Disseminated necrotic areas were detected in enlarged lymph-nodes, liver and spleen. Vertebral granulomas within lytic areas in T10-T11 and L2-L3, were observed. Cultures inoculated with samples obtained from lymph-node FNAB and bioptic material from necropsied organs revealed the presence of pure cultures of Penicillium, subsequently identified as P. purpurogenum. Apart from female GSD's suspected predisposition to disseminated mycoses described in literature, no other predisposing factors were ascertained in this case.

Differentiation of sex chromosomes and karyotypic evolution in the eye-lid geckos (Squamata: Gekkota: Eublepharidae), a group with different modes of sex determination.

PubMed

Pokorná, Martina; Rábová, Marie; Ráb, Petr; Ferguson-Smith, Malcolm A; Rens, Willem; Kratochvíl, Lukáš

2010-11-01

The eyelid geckos (family Eublepharidae) include both species with temperature-dependent sex determination and species where genotypic sex determination (GSD) was suggested based on the observation of equal sex ratios at several incubation temperatures. In this study, we present data on karyotypes and chromosomal characteristics in 12 species (Aeluroscalabotes felinus, Coleonyx brevis, Coleonyx elegans, Coleonyx variegatus, Eublepharis angramainyu, Eublepharis macularius, Goniurosaurus araneus, Goniurosaurus lichtenfelderi, Goniurosaurus luii, Goniurosaurus splendens, Hemitheconyx caudicinctus, and Holodactylus africanus) covering all genera of the family, and search for the presence of heteromorphic sex chromosomes. Phylogenetic mapping of chromosomal changes showed a long evolutionary stasis of karyotypes with all acrocentric chromosomes followed by numerous chromosomal rearrangements in the ancestors of two lineages. We have found heteromorphic sex chromosomes in only one species, which suggests that sex chromosomes in most GSD species of the eyelid geckos are not morphologically differentiated. The sexual difference in karyotype was detected only in C. elegans which has a multiple sex chromosome system (X(1)X(2)Y). The metacentric Y chromosome evolved most likely via centric fusion of two acrocentric chromosomes involving loss of interstitial telomeric sequences. We conclude that the eyelid geckos exhibit diversity in sex determination ranging from the absence of any sexual differences to heteromorphic sex chromosomes, which makes them an interesting system for exploring the evolutionary origin of sexually dimorphic genomes.

Image Quality Assessment of High-Resolution Satellite Images with Mtf-Based Fuzzy Comprehensive Evaluation Method

NASA Astrophysics Data System (ADS)

Wu, Z.; Luo, Z.; Zhang, Y.; Guo, F.; He, L.

2018-04-01

A Modulation Transfer Function (MTF)-based fuzzy comprehensive evaluation method was proposed in this paper for the purpose of evaluating high-resolution satellite image quality. To establish the factor set, two MTF features and seven radiant features were extracted from the knife-edge region of image patch, which included Nyquist, MTF0.5, entropy, peak signal to noise ratio (PSNR), average difference, edge intensity, average gradient, contrast and ground spatial distance (GSD). After analyzing the statistical distribution of above features, a fuzzy evaluation threshold table and fuzzy evaluation membership functions was established. The experiments for comprehensive quality assessment of different natural and artificial objects was done with GF2 image patches. The results showed that the calibration field image has the highest quality scores. The water image has closest image quality to the calibration field, quality of building image is a little poor than water image, but much higher than farmland image. In order to test the influence of different features on quality evaluation, the experiment with different weights were tested on GF2 and SPOT7 images. The results showed that different weights correspond different evaluating effectiveness. In the case of setting up the weights of edge features and GSD, the image quality of GF2 is better than SPOT7. However, when setting MTF and PSNR as main factor, the image quality of SPOT7 is better than GF2.

METimage: an innovative multi-spectral imaging radiometer for the EUMETSAT polar system follow-on satellite mission

NASA Astrophysics Data System (ADS)

Alpers, Matthias; Brüns, Christian; Pillukat, Alexander

2017-11-01

The evolving needs of the meteorological community concerning the EUMETSAT Polar System follow-on satellite mission (Post-EPS) require the development of a high-performance multi-spectral imaging radiometer. Recognizing these needs, Jena Optronik GmbH proposed an innovative instrument concept, which comprises a high flexibility to adapt to user requirements as a very important feature. Core parameters like ground sampling distance (GSD), number and width of spectral channels, signal-to-noise ratio, polarization control and calibration facilities can be chosen in a wide range without changing the basic instrument configuration. Core item of the METimage instrument is a rotating telescope scanner to cover the large swath width of about 2800 km, which all polar platforms need for global coverage. The de-rotated image facilitates use of in-field spectral channel separation, which allows tailoring individual channel GSD (ground sampling distance) and features like TDI (time delay and integration). State-of-the-art detector arrays and readout electronics can easily be employed. Currently, the German DLR Space Agency, Jena- Optronik GmbH and AIM Infrarot Module GmbH work together implementing core assemblies of METimage: the rotating telescope scanner and the infrared detectors. The METimage instrument phase B study was kicked-off in September 2008. Germany intents to provide METimage as an in-kind contribution of the first METimage flight model to the EUMETSAT Post-EPS Programme.

The Peoples Liberation Army and Contingency Planning in China

DTIC Science & Technology

2015-01-01

House [军事科学出版社], 1998), 93–94. 59 Liu Jian [刘建], “ Wireless Technologies: Military Applications of Wireless Sensor Networks” [无线技术：无线传感器网络在军事的应用...Weinan (Shaanxi Province), prob- ably fuses space tracking data from sensors managed by the GSD Technical Reconnaissance Department, PLA Air Force (PLAAF...would probably integrate sensor information from strategic- and operational-level intelligence, surveillance, and reconnaissance assets. A firepower

ASPRS Digital Imagery Guideline Image Gallery Discussion

NASA Technical Reports Server (NTRS)

Ryan, Robert

2002-01-01

The objectives of the image gallery are to 1) give users and providers a simple means of identifying appropriate imagery for a given application/feature extraction; and 2) define imagery sufficiently to be described in engineering and acquisition terms. This viewgraph presentation includes a discussion of edge response and aliasing for image processing, and a series of images illustrating the effects of signal to noise ratio (SNR) on images. Another series of images illustrates how images are affected by varying the ground sample distances (GSD).

Hierarchically-Clustered Structures: Formation During Colloidal Processing and Evolution During Sintering

DTIC Science & Technology

1990-12-04

18) E. Carnal and A Mocellin , " A Topological Model for Plane Sections of Polycrystals," Submitted to Acta. Metal.. 19) S. Fakuda, R. Kikuchi, and I...obtained from his CCD and the perimeter law matched the GSD generated by the Exxon group. Mocellin and his coworkers in Switzerland 32,33 (the Swiss...cellular networks," Phil. Mag B52(3), 795 (1985). 32). M. Blanc, A. Mocellin , "Grain coordination in plane sections of polycrystals," Acta Metall. 27

One year in orbit of the first Geostationary Ocean Colour Imager (GOCI)

NASA Astrophysics Data System (ADS)

Faure, François; Coste, Pierre; Benchetrit, Thierry; Kang, Gm Sil; Kim, Han-dol

2017-11-01

Geostationary Ocean Colour Imager (GOCI) is the first Ocean Colour Imager to operate from a Geostationary Orbit. It was developed by Astrium SAS under KARI contract in about 3 years between mid 2005 and October 2008 and integrated on-board COMS satellite end 2008 aside the COMS Meteo Imager (MI). COMS satellite was launched in June 2010 and the in-orbit commissioning tests were completed in beginning of 2011. The mission is designed to significantly improve ocean observation in complement with low orbit service by providing high frequency coverage. The GOCI is designed to provide multi-spectral data to detect, monitor, quantify, and predict short-term changes of coastal ocean environment for marine science research and application purpose. Target area for the GOCI observation in the COMS satellite covers a large 2500 x 2500 km2 sea area around the Korean Peninsula, with an average Ground sampling distance (GSD) of 500m, corresponding to a NADIR GSD of 360m. The presentation will shortly recall the mission objectives and major instrument requirements, and then present the results of inorbit testing and validations. All functions and in particular the CMOS detector matrix operate nominally. Performances evaluated in orbit (SNR, MTF, etc.) show results above the requirements. Finally, in-orbit calibrations using the sun diffuser provide very satisfactory consistency with the ground characterisation. GOCI is now delivering operational products and proving the interest of Geo observation in the Ocean Colour applications

Stability of total nutrient admixtures with lipid injectable emulsions in glass versus plastic packaging.

PubMed

Driscoll, David F; Silvestri, Anthony P; Bistrian, Bruce R; Mikrut, Bernard A

2007-02-15

The physical stability of two emulsions compounded as part of a total nutrient admixture (TNA) was studied in lipids packaged in either glass or plastic containers. Five weight-based adult TNA formulations that were designed to meet the full nutritional needs of adults with body weights between 40 and 80 kg were studied. Triplicate preparations of each TNA were assessed over 30 hours at room temperature by applying currently proposed United States Pharmacopeia (USP) criteria for mean droplet diameter, large-diameter tail, and globule-size distribution (GSD) for lipid injectable emulsions. In accordance with conditions set forth in USP chapter 729, the higher levels of volume-weighted percent of fat exceeding 5 microm (PFAT(5)) should not exceed 0.05% of the total lipid concentration. Significant differences were noted among TNA admixtures based on whether the lipid emulsion product was manufactured in glass or plastic. The plastic-contained TNAs failed the proposed USP methods for large-diameter fat globules in all formulations from the outset, and 60% had significant growth in large-diameter fat globules over time. In contrast, glass-contained TNAs were stable throughout and in all cases would have passed proposed USP limits. Certain lipid injectable emulsions packaged in plastic containers have baseline abnormal GSD profiles compared with those packaged in glass containers. When used to compound TNAs, the abnormal profile worsens and produces less stable TNAs than those compounded with lipid injectable emulsions packaged in glass containers.

Globule-size distribution in injectable 20% lipid emulsions: Compliance with USP requirements.

PubMed

Driscoll, David F

2007-10-01

The compliance of injectable 20% lipid emulsions with the globule-size limits in chapter 729 of the U.S. Pharmacopeia (USP) was examined. As established in chapter 729, dynamic light scattering was applied to determine mean droplet diameter (MDD), with an upper limit of 500 nm. Light obscuration was used to determine the size of fat globules found in the large-diameter tail, expressed as the volume-weighted percent fat exceeding 5 microm (PFAT(5)), with an upper limit of 0.05%. Compliance of seven different emulsions, six of which were stored in plastic bags, with USP limits was assessed. To avoid reaching coincidence limits during the application of method II from overly concentrated emulsion samples, a variable dilution scheme was used to optimize the globule-size measurements for each emulsion. One-way analysis of variance of globule-size distribution (GSD) data was conducted if any results of method I or II exceeded the respective upper limits. Most injectable lipid emulsions complied with limits established by USP chapter 729, with the exception of those of one manufacturer, which failed limits as proposed for to meet the PFAT(5) three of the emulsions tested. In contrast, all others studied (one packaged in glass and three packaged in plastic) met both criteria. Among seven injectable lipid emulsions tested for GSD, all met USP chapter 729 MDD requirements and three, all from the same manufacturer and packaged in plastic, did not meet PFAT(5) requirements.

The influence of initial atomized droplet size on residual particle size from pressurized metered dose inhalers.

PubMed

Sheth, Poonam; Stein, Stephen W; Myrdal, Paul B

2013-10-15

Pressurized metered dose inhalers (pMDIs) are widely used for the treatment of diseases of the lung, including asthma and chronic obstructive pulmonary disease. The mass median aerodynamic diameter of the residual particles (MMADR) delivered from a pMDI plays a key role in determining the amount and location of drug deposition in the lung and thereby the efficacy of the inhaler. The mass median diameter of the initial droplets (MMDI), upon atomization of a formulation, is a significant factor influencing the final particle size. The purpose of this study was to evaluate the extent that MMDI and initial droplet geometric standard deviation (GSD) influence the residual aerodynamic particle size distribution (APSDR) of solution and suspension formulations. From 48 solution pMDI configurations with varying ethanol concentrations, valve sizes and actuator orifice diameters, it was experimentally found that the effective MMDI ranged from 7.8 to 13.3 μm. Subsequently, computational methods were utilized to determine the influence of MMDI on MMADR, by modulating the MMDI for solution and suspension pMDIs. For solution HFA-134a formulations of 0.5% drug in 10% ethanol, varying the MMDI from 7.5 to 13.5 μm increased the MMADR from 1.4 to 2.5 μm. For a suspension formulation with a representative particle size distribution of micronized drug (MMAD=2.5 μm, GSD=1.8), the same increase in MMDI resulted in an increase in the MMADR from 2.7 to only 3.3 μm. Hence, the same increase in MMDI resulted in a 79% increase in MMADR for the solution formulation compared to only a 22% increase for the suspension formulation. Similar trends were obtained for a range of drug concentrations and input micronized drug sizes. Thus, APSDR is more sensitive to changes in MMDI for solution formulations than suspension formulations; however, there are situations in which hypothetically small micronized drug in suspension (e.g. 500 nm MMAD) could resemble trends observed for solution formulations. Furthermore, the relationship between APSDR and drug concentration and MMDI is predictable for solution pMDIs, but this is not as straightforward for suspension formulations. In addition, the MMADR was relatively insensitive to changes in initial droplet GSD (from 1.6 to 2.0) and the solution and suspension pMDI residual particle GSDs were essentially identical to the initial droplet GSDs. Copyright © 2013 Elsevier B.V. All rights reserved.

Super-resolution optical imaging and magnetometry using NV centers in diamond

NASA Astrophysics Data System (ADS)

Jaskula, Jean-Christophe; Trifonov, Alexei; Glenn, David; Bar-Gill, Nir; Walsworth, Ronald

2013-05-01

We report progress done on the development and application of depletion-based techniques for super-resolution (nanoscale) optical imaging and magnetometry using NV centers in diamond. In particulare we are integrating stimulated emission depletion (STED) and ground state depletion (GSD) imaging techniques with advanced pulsed sequences for AC magnetometry. NV centers in diamond do not bleach under optical excitation, have long-lived electronic spin coherence and spin-state-dependent fluorescence, and are not biotoxic. Thus NV-diamond has great potential in quantum science and as a nanoscale magnetic biosensor.

Airborne concentrations of methylene diphenyl diisocyanate (MDI) in North American wood mills during the manufacturing of oriented strand board (OSB).

PubMed

Karoly, William J; Flatley, John J; Stevenson, Ralph D; Bowers, John D

2004-12-01

Air monitoring data were collected from industrial hygiene surveys over an 8-year period in oriented strand board (OSB) mills. Personal samples were taken to evaluate potential employee exposures to MDI. Area samples were taken to determine the effectiveness of control measures used in the mills to prevent fugitive emissions of wood dust, MDI, and MDI-coated wood dust from the OSB manufacturing process. Personal sampling results (578 samples covering 11 different job categories) ranged from 0.0002-0.524 mg/m3, with a GM = 0.001 and GSD = 3.71. Area sampling results (1657 samples covering 14 stationary locations in the mills) ranged from 0.0002-2.5 mg/m3, with a GM = 0.004 and GSD = 5.52. The statistical range of the data suggests high variability. While exposures to MDI above the established limits (0.051 mg/m3, 8-hour time-weighted average, 0.2 mg/m3, ceiling) can and do occur when engineering controls are not maintained and/or proper work practices and personal protective equipment are not followed/used for certain high exposure potential tasks, the data indicate that over 97% of the personal and 92% of the area sampling results are less than 0.051 mg/m3. Wipe testing was performed to determine the presence of removable, unreacted diisocyanates (NCO functional groups)from various surfaces. Positive results were found in about 13% of the wipe tests on surfaces confined to the blender, forming line, and hopper deck process areas.

"I am yet to encounter any survey that actually reflects my life": a qualitative study of inclusivity in sexual health research.

PubMed

Carrotte, Elise R; Vella, Alyce M; Bowring, Anna L; Douglass, Caitlin; Hellard, Margaret E; Lim, Megan S C

2016-07-27

Heteronormativity describes a set of norms and assumptions pertaining to heterosexual identities and binary gender. In 2015, we conducted our annual Sex, Drugs and Rock'n'Roll study, an online health survey of over 1000 Victorians aged 15-29 years. Feedback from participants suggested that our survey contained heteronormative language. In response to this, we aimed to make inclusive changes to our survey via consultation with young gender and sexually diverse (GSD) people. We conducted two semi-structured focus groups in Melbourne with a total of 16 participants (age range: 21-28 years). Participants were mostly cisgender women, and there were two transgender participants and one non-binary participant. Participants also had a range of sexual identities including lesbian, queer, bisexual, pansexual, and asexual. Focus group discussions were transcribed verbatim and analysed thematically. Most participants indicated heteronormativity affects their lives in multiple ways, noting its impacts on access to sexual healthcare, invalidating sexual experiences and miscommunication in forms and surveys. Overall, participants emphasised the need for sexual health research to avoid assumptions about behaviour, to be clear and eliminate question ambiguity and avoiding treating gender as binary. Participants also discussed how the Sex, Drugs and Rock'n'Roll survey could address a range of sexual behaviours and experiences, rather than focusing on penetrative sex, which many participants found invalidating. Our findings have important implications for future health surveys aimed at general populations. We present recommendations that encourage research to be more inclusive to ensure data collection from GSD participants is respectful and rigorous.

Correlation of nutritional parameters of gallbladder cancer patients.

PubMed

Rai, Arundhati; Tewari, Mallika; Mohapatra, S C; Shukla, H S

2006-06-15

Gallbladder cancer (GBC) is a highly fatal disease with poor prognosis and 5-year survival <5%. Weight loss and nutritional deterioration are associated with adverse outcomes in terms of cancer prognosis. Protein-calorie malnutrition is the single most common secondary diagnosis in a patient with cancer, and is a direct consequence of the anorexia of malignancy and altered host metabolism induced by the tumor. The present study of nutritional assessment is of particular interest for it helps in better understanding the extent of malnutrition in patients of GBC. A case-control study was designed comprising of 153 cases of GBC and 153 controls of gallstone disease (GSD). To assess the nutritional status of the GBC patients, anthropometric measurements such as height, weight, mid arm circumference, and skinfold thickness were recorded together with the biochemical parameters and their nutrient intake. The present study showed that GBC influences the nutritional status of the patients. Forty-three percent of GBC patients were malnourished with low body mass index (BMI). A significant reduction in all the anthropometric measures was observed for GBC patients compared to those with GSD. GBC patients had significantly low hemoglobin and serum albumin levels compared to the control group. The hemoglobin levels in case and control groups were 10.87 g/dl (+/-1.81 SD) and 11.62 g/dl (+/-1.89 SD), respectively (P < 0.001). Intake of almost all the nutrients was far below the recommendations of Indian Council of Medical Research. GBC patients had anorexia and weight loss. Copyright 2006 Wiley-Liss, Inc.

Degenerative myelopathy in German Shepherd Dog: comparison of two molecular assays for the identification of the SOD1:c.118G>A mutation.

PubMed

Capucchio, Maria Teresa; Spalenza, Veronica; Biasibetti, Elena; Bottero, Maria Teresa; Rasero, Roberto; Dalmasso, Alessandra; Sacchi, Paola

2014-02-01

Degenerative myelopathy (DM) is a late-onset, slowly progressive degeneration of spinal cord white matter which is reported primarily in large breed dogs. The missense mutation SOD1:c.118G>A is associated with this pathology in several dog breeds, including the German Shepherd Dog (GSD). The aims of the present study were to develop a tool for the rapid screening of the SOD1 mutation site in dogs and to evaluate the association of the polymorphism with DM in the German Shepherd breed. Two different techniques were compared: a minisequencing test and a real-time pcr allelic discrimination assay. Both approaches resulted effective and efficient. A sample of 47 dogs were examined. Ten subjects presented the symptoms of the illness; for one of them the diagnosis was confirmed by postmortem investigations and it resulted to be an A/A homozygote. In another clinically suspected dog, heterozygote A/G, the histopathological examination of the medulla showed moderate axon and myelin degenerative changes. GSD shows a frequency of the mutant allele equal to 0.17, quite high being a high-risk allele. Because canine DM has a late onset in adulthood and homozygous mutant dogs are likely as fertile as other genotypes, the natural selection is mild and the mutant allele may reach high frequencies. A diagnostic test, easy to implement, may contribute to control the gene diffusion in populations. The SOD1:c.118G>A mutation could be a useful marker for breeding strategies intending to reduce the incidence of DM.

Silica dust exposures during selected construction activities.

PubMed

Flanagan, Mary Ellen; Seixas, Noah; Majar, Maria; Camp, Janice; Morgan, Michael

2003-01-01

This study characterized exposure for dust-producing construction tasks. Eight common construction tasks were evaluated for quartz and respirable dust exposure by collecting 113 personal task period samples for cleanup; demolition with handheld tools; concrete cutting; concrete mixing; tuck-point grinding; surface grinding; sacking and patching concrete; and concrete floor sanding using both time-integrating filter samples and direct-reading respirable dust monitors. The geometric mean quartz concentration was 0.10 mg/m(3) (geometric standard deviation [GSD]=4.88) for all run time samples, with 71% exceeding the threshold limit value. Activities with the highest exposures were surface grinding, tuck-point grinding, and concrete demolition (GM[GSD] of 0.63[4.12], 0.22[1.94], and 0.10[2.60], respectively). Factors recorded each minute were task, tool, work area, respiratory protection and controls used, estimated cross draft, and whether anyone nearby was making dust. Factors important to exposure included tool used, work area configuration, controls employed, cross draft, and in some cases nearby dust. More protective respirators were employed as quartz concentration increased, although respiratory protection was found to be inadequate for 42% of exposures. Controls were employed for only 12% of samples. Exposures were reduced with three controls: box fan for surface grinding and floor sanding, and vacuum/shroud for surface grinding, with reductions of 57, 50, and 71%, respectively. Exposures were higher for sweeping compound, box fan for cleanup, ducted fan dilution, and wetted substrate. Construction masons and laborers are frequently overexposed to silica. The usual protection method, respirators, was not always adequate, and engineering control use was infrequent and often ineffective.

Hypoglycaemia related to inherited metabolic diseases in adults

PubMed Central

2012-01-01

In non-diabetic adult patients, hypoglycaemia may be related to drugs, critical illness, cortisol or glucagon insufficiency, non-islet cell tumour, insulinoma, or it may be surreptitious. Nevertheless, some hypoglycaemic episodes remain unexplained, and inborn errors of metabolism (IEM) should be considered, particularly in cases of multisystemic involvement. In children, IEM are considered a differential diagnosis in cases of hypoglycaemia. In adulthood, IEM-related hypoglycaemia can persist in a previously diagnosed childhood disease. Hypoglycaemia may sometimes be a presenting sign of the IEM. Short stature, hepatomegaly, hypogonadism, dysmorphia or muscular symptoms are signs suggestive of IEM-related hypoglycaemia. In both adults and children, hypoglycaemia can be clinically classified according to its timing. Postprandial hypoglycaemia can be an indicator of either endogenous hyperinsulinism linked to non-insulinoma pancreatogenic hypoglycaemia syndrome (NIPHS, unknown incidence in adults) or very rarely, inherited fructose intolerance. Glucokinase-activating mutations (one family) are the only genetic disorder responsible for NIPH in adults that has been clearly identified so far. Exercise-induced hyperinsulinism is linked to an activating mutation of the monocarboxylate transporter 1 (one family). Fasting hypoglycaemia may be caused by IEM that were already diagnosed in childhood and persist into adulthood: glycogen storage disease (GSD) type I, III, 0, VI and IX; glucose transporter 2 deficiency; fatty acid oxidation; ketogenesis disorders; and gluconeogenesis disorders. Fasting hypoglycaemia in adulthood can also be a rare presenting sign of an IEM, especially in GSD type III, fatty acid oxidation [medium-chain acyl-CoA dehydrogenase (MCAD), ketogenesis disorders (3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) lyase deficiency, and gluconeogenesis disorders (fructose-1,6-biphosphatase deficiency)]. PMID:22587661

Do downscaled general circulation models reliably simulate historical climatic conditions?

USGS Publications Warehouse

Bock, Andrew R.; Hay, Lauren E.; McCabe, Gregory J.; Markstrom, Steven L.; Atkinson, R. Dwight

2018-01-01

The accuracy of statistically downscaled (SD) general circulation model (GCM) simulations of monthly surface climate for historical conditions (1950–2005) was assessed for the conterminous United States (CONUS). The SD monthly precipitation (PPT) and temperature (TAVE) from 95 GCMs from phases 3 and 5 of the Coupled Model Intercomparison Project (CMIP3 and CMIP5) were used as inputs to a monthly water balance model (MWBM). Distributions of MWBM input (PPT and TAVE) and output [runoff (RUN)] variables derived from gridded station data (GSD) and historical SD climate were compared using the Kolmogorov–Smirnov (KS) test For all three variables considered, the KS test results showed that variables simulated using CMIP5 generally are more reliable than those derived from CMIP3, likely due to improvements in PPT simulations. At most locations across the CONUS, the largest differences between GSD and SD PPT and RUN occurred in the lowest part of the distributions (i.e., low-flow RUN and low-magnitude PPT). Results indicate that for the majority of the CONUS, there are downscaled GCMs that can reliably simulate historical climatic conditions. But, in some geographic locations, none of the SD GCMs replicated historical conditions for two of the three variables (PPT and RUN) based on the KS test, with a significance level of 0.05. In these locations, improved GCM simulations of PPT are needed to more reliably estimate components of the hydrologic cycle. Simple metrics and statistical tests, such as those described here, can provide an initial set of criteria to help simplify GCM selection.

Internet Based Remote Operations

NASA Technical Reports Server (NTRS)

Chamberlain, James

1999-01-01

This is the Final Report for the Internet Based Remote Operations Contract, has performed payload operations research support tasks March 1999 through September 1999. These tasks support the GSD goal of developing a secure, inexpensive data, voice, and video mission communications capability between remote payload investigators and the NASA payload operations team in the International Space Station (ISS) era. AZTek has provided feedback from the NASA payload community by utilizing its extensive payload development and operations experience to test and evaluate remote payload operations systems. AZTek has focused on use of the "public Internet" and inexpensive, Commercial-off-the-shelf (COTS) Internet-based tools that would most benefit "small" (e.g., $2 Million or less) payloads and small developers without permanent remote operations facilities. Such projects have limited budgets to support installation and development of high-speed dedicated communications links and high-end, custom ground support equipment and software. The primary conclusions of the study are as follows: (1) The trend of using Internet technology for "live" collaborative applications such as telescience will continue. The GSD-developed data and voice capabilities continued to work well over the "public" Internet during this period. 2. Transmitting multiple voice streams from a voice-conferencing server to a client PC to be mixed and played on the PC is feasible. 3. There are two classes of voice vendors in the market: - Large traditional phone equipment vendors pursuing integration of PSTN with Internet, and Small Internet startups.The key to selecting a vendor will be to find a company sufficiently large and established to provide a base voice-conferencing software product line for the next several years.

Disposition of americium-241 oxide following inhalation by beagles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Craig, D.K.; Park, J.F.; Powers, G.J.

1979-06-01

The disposition of americium-241 in beagles was followed for up to 810 days after a single inhalation exposure to /sup 241/AmO/sub 2/ aerosols having an activity median aerodynamic diameter (AMAD) of 1.3 ..mu..m and a geometric standard deviation 8GSD) of 1.8 for medium (approx. 40 nCi/liter) and high (approx. 340 nCi/liter) concentrations. At low concentrations (< 1 nCi/liter), the aerosols were smaller (AMAD approx. 0.6 ..mu..m) and the distribution broader (GSD approx. 2.6). Excreta were analyzed for up to 30 days postexposure and where appropriate, during the last week before sacrifice. Tissue analyses for /sup 241/Am were conducted on groupsmore » of three dogs sacrificed 10, 30, 90, 270, and 810 days postexposure. Forty percent of the final body burden of /sup 241/Am was located in tissues other than the lung parenchyma by 10 days postexposure and only 6% remained in the lung by 810 days postexposure. Translocation was primarilly to the liver and skeleton, with roughly equal fractions of the final body burden in each at all postexposure sacrifice times. There in the gonads was negligible (< 0.05%) for these dogs. Both the rate of translocation and the organ distribution as a function of time are different from those found in dogs that inhaled /sup 238/PuO/sub 2/ or /sup 239/PuO/sub 2/. Therefore it is not appropriate to use data derived from /sup 239/PuO/sub 2/ experiments or observations to predict the behavior of other transuranic isotopes and elements.« less

Comparison of hospital room surface disinfection using a novel ultraviolet germicidal irradiation (UVGI) generator.

PubMed

Jelden, Katelyn C; Gibbs, Shawn G; Smith, Philip W; Hewlett, Angela L; Iwen, Peter C; Schmid, Kendra K; Lowe, John J

2016-09-01

The estimated 721,800 hospital acquired infections per year in the United States have necessitated development of novel environmental decontamination technologies such as ultraviolet germicidal irradiation (UVGI). This study evaluated the efficacy of a novel, portable UVGI generator (the TORCH, ChlorDiSys Solutions, Inc., Lebanon, NJ) to disinfect surface coupons composed of plastic from a bedrail, stainless steel, chrome-plated light switch cover, and a porcelain tile that were inoculated with methicillin-resistant Staphylococcus aureus (MRSA) or vancomycin-resistant Enterococcus faecalis (VRE). Each surface type was placed at 6 different sites within a hospital room and treated by 10-min ultraviolet-C (UVC) exposures using the TORCH with doses ranging from 0-688 mJ/cm(2) between sites. Organism reductions were compared with untreated surface coupons as controls. Overall, UVGI significantly reduced MRSA by an average of 4.6 log10 (GSD: 1.7 log10, 77% inactivation, p < 0.0001) and VRE by an average of 3.9 log10 (GSD: 1.7 log10, 65% inactivation, p < 0.0001). MRSA on bedrail was reduced significantly (p < 0.0001) less than on other surfaces, while VRE was reduced significantly less on chrome (p = 0.0004) and stainless steel (p = 0.0012) than porcelain tile. Organisms out of direct line of sight of the UVC generator were reduced significantly less (p < 0.0001) than those directly in line of sight. UVGI was found an effective method to inactivate nosocomial pathogens on surfaces evaluated within the hospital environment in direct line of sight of UVGI treatment with variation between organism and surface types.

Patient doses in the healing arts

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

Determinations of radiation doses to patients from x-ray procedures and radiopharmaceuticals are detailed in this chapter. Instructions are given for estimating doses from x-ray procedures. For selected pediatric procedures, the methodology developed by the Food and Drug Administration is presented. The effect of testicular and ovarian shielding is illustrated in tabular form. Estimates of the Genetically Significant Dose (GSD) and mean annual bone marrow dose from diagnostic x-ray examinations are presented for the US populations (1990). This chapter also provides tables of patient doses from selected nuclear medicine procedures and estimates of fetal doses from {sup 131}I.

Fungal DNA in hotel rooms in Europe and Asia--associations with latitude, precipitation, building data, room characteristics and hotel ranking.

PubMed

Norbäck, Dan; Cai, Gui-Hong

2011-10-01

There is little information on the indoor environment in hotels. Analysis of fungal DNA by quantitative PCR (qPCR) is a new method which can detect general and specific sequences. Dust was collected through swab sampling of door frames in 69 hotel rooms in 20 countries in Europe and Asia (2007-2009). Five sequences were detected by qPCR: total fungal DNA, Aspergillus and Penicillium DNA (Asp/Pen DNA), Aspergillus versicolor (A. versicolor DNA), Stachybotrys chartarum (S. chartarum DNA) and Streptomyces spp. (Streptomyces DNA). Associations were analysed by multiple linear regression. Total fungal DNA (GM = 1.08 × 10(8) cell equivalents m(-2); GSD = 6.36) and Asp/Pen DNA (GM = 1.79 × 10(7) cell equivalents m(-2); GSD = 10.12) were detected in all rooms. A. versicolor DNA, S. chartarum DNA and Streptomyces DNA were detected in 84%, 28% and 47% of the samples. In total, 20% of the rooms had observed dampness/mould, and 30% had odour. Low latitude (range 1.5-64.2 degrees) was a predictor of Asp/Pen DNA. Seaside location, lack of mechanical ventilation, and dampness or mould were other predictors of total fungal DNA and Asp/Pen DNA. Hotel ranking (Trip Advisor) or self-rated quality of the interior of the hotel room was a predictor of total fungal DNA, A. versicolor DNA and Streptomyces DNA. Odour was a predictor of S. chartarum DNA. In conclusion, fungal DNA in swab samples from hotel rooms was related to latitude, seaside location, ventilation, visible dampness and indoor mould growth. Hotels in tropical areas may have 10-100 times higher levels of common moulds such as Aspergillus and Penicillium species, as compared to a temperate climate zone.

The role of national and international geospatial data sources in the management of natural disasters

NASA Astrophysics Data System (ADS)

Kayi, A.; Erdogan, M.; Yilmaz, A.

2014-11-01

An earthquake occurred at Van City on 23 October 2011 at 13:41 local time. The magnitude, moment magnitude and depth of earthquake were respectively MI:6.7, Mw:7.0 and 19.07 km. Van city centre and its surrounding villages were affected from this destructive earthquake. Many buildings were ruined and approximately 600 people died. Acquisition and use of geospatial data is very important and crucial for the management of such kind of natural disasters. In this paper, the role of national and international geospatial data in the management of Van earthquake is investigated.. With an international collaboration with Charter, pre and post-earthquake satellite images were acquired in 24 hours following the Earthquake. Also General Command of Mapping (GCM), the national mapping agency of Turkey, produced the high resolution multispectral orthophotos of the region. Charter presented the orthophotos through 26-28 October 2012. Just after the earthquake with a quick reaction, GCM made the flight planning of the 1296 km2 disaster area to acquire aerial photos. The aerial photos were acquired on 24 October 2012 (one day after the earthquake) by UltraCamX large format digital aerial camera. 152 images were taken with 30 cm ground sample distance (GSD) by %30 sidelap and %60 overlap. In the evening of same flight day, orthophotos were produced without ground control points by direct georeferencing and GCM supplied the orthophotos to the disaster management authorities. Also 45 cm GSD archive orthophotos, acquired in 2010, were used as a reference in order to find out the effects of the disaster. The subjects written here do not represent the ideas of Turkish Armed Forces.

MACSAT - A Near Equatorial Earth Observation Mission

NASA Astrophysics Data System (ADS)

Kim, B. J.; Park, S.; Kim, E.-E.; Park, W.; Chang, H.; Seon, J.

MACSAT mission was initiated by Malaysia to launch a high-resolution remote sensing satellite into Near Equatorial Orbit (NEO). Due to its geographical location, Malaysia can have large benefits from NEO satellite operation. From the baseline circular orbit of 685 km altitude with 7 degrees of inclination, the neighboring regions around Malaysian territory can be frequently monitored. The equatorial environment around the globe can also be regularly observed with unique revisit characteristics. The primary mission objective of MACSAT program is to develop and validate technologies for a near equatorial orbit remote sensing satellite system. MACSAT is optimally designed to accommodate an electro-optic Earth observation payload, Medium-sized Aperture Camera (MAC). Malaysian and Korean joint engineering teams are formed for the effective implementation of the satellite system. An integrated team approach is adopted for the joint development for MACSAT. MAC is a pushbroom type camera with 2.5 m of Ground Sampling Distance (GSD) in panchromatic band and 5 m of GSD in four multi-spectral bands. The satellite platform is a mini-class satellite. Including MAC payload, the satellite weighs under 200 kg. Spacecraft bus is designed optimally to support payload operations during 3 years of mission life. The payload has 20 km of swath width with +/- 30 o of tilting capability. 32 Gbits of solid state recorder is implemented as the mass image storage. The ground element is an integrated ground station for mission control and payload operation. It is equipped with S- band up/down link for commanding and telemetry reception as well as 30 Mbps class X-band down link for image reception and processing. The MACSAT system is capable of generating 1:25,000-scale image maps. It is also anticipated to have capability for cross-track stereo imaging for Digital elevation Model (DEM) generation.

Plant Uptake of Organic Pollutants from Soil: A Critical Review ofBioconcentration Estimates Based on Modelsand Experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

McKone, Thomas E.; Maddalena, Randy L.

2007-01-01

The role of terrestrial vegetation in transferring chemicals from soil and air into specific plant tissues (stems, leaves, roots, etc.) is still not well characterized. We provide here a critical review of plant-to-soil bioconcentration ratio (BCR) estimates based on models and experimental data. This review includes the conceptual and theoretical formulations of the bioconcentration ratio, constructing and calibrating empirical and mathematical algorithms to describe this ratio and the experimental data used to quantify BCRs and calibrate the model performance. We first evaluate the theoretical basis for the BCR concept and BCR models and consider how lack of knowledge and datamore » limits reliability and consistency of BCR estimates. We next consider alternate modeling strategies for BCR. A key focus of this evaluation is the relative contributions to overall uncertainty from model uncertainty versus variability in the experimental data used to develop and test the models. As a case study, we consider a single chemical, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and focus on variability of bioconcentration measurements obtained from 81 experiments with different plant species, different plant tissues, different experimental conditions, and different methods for reporting concentrations in the soil and plant tissues. We use these observations to evaluate both the magnitude of experimental variability in plant bioconcentration and compare this to model uncertainty. Among these 81 measurements, the variation of the plant/soil BCR has a geometric standard deviation (GSD) of 3.5 and a coefficient of variability (CV-ratio of arithmetic standard deviation to mean) of 1.7. These variations are significant but low relative to model uncertainties--which have an estimated GSD of 10 with a corresponding CV of 14.« less

ELF magnetic fields in electric and gasoline-powered vehicles.

PubMed

Tell, R A; Sias, G; Smith, J; Sahl, J; Kavet, R

2013-02-01

We conducted a pilot study to assess magnetic field levels in electric compared to gasoline-powered vehicles, and established a methodology that would provide valid data for further assessments. The sample consisted of 14 vehicles, all manufactured between January 2000 and April 2009; 6 were gasoline-powered vehicles and 8 were electric vehicles of various types. Of the eight models available, three were represented by a gasoline-powered vehicle and at least one electric vehicle, enabling intra-model comparisons. Vehicles were driven over a 16.3 km test route. Each vehicle was equipped with six EMDEX Lite broadband meters with a 40-1,000 Hz bandwidth programmed to sample every 4 s. Standard statistical testing was based on the fact that the autocorrelation statistic damped quickly with time. For seven electric cars, the geometric mean (GM) of all measurements (N = 18,318) was 0.095 µT with a geometric standard deviation (GSD) of 2.66, compared to 0.051 µT (N = 9,301; GSD = 2.11) for four gasoline-powered cars (P < 0.0001). Using the data from a previous exposure assessment of residential exposure in eight geographic regions in the United States as a basis for comparison (N = 218), the broadband magnetic fields in electric vehicles covered the same range as personal exposure levels recorded in that study. All fields measured in all vehicles were much less than the exposure limits published by the International Commission on Non-Ionizing Radiation Protection (ICNIRP) and the Institute of Electrical and Electronics Engineers (IEEE). Future studies should include larger sample sizes representative of a greater cross-section of electric-type vehicles. Copyright © 2012 Wiley Periodicals, Inc.

A Satellite-Based Imaging Instrumentation Concept for Hyperspectral Thermal Remote Sensing.

PubMed

Udelhoven, Thomas; Schlerf, Martin; Segl, Karl; Mallick, Kaniska; Bossung, Christian; Retzlaff, Rebecca; Rock, Gilles; Fischer, Peter; Müller, Andreas; Storch, Tobias; Eisele, Andreas; Weise, Dennis; Hupfer, Werner; Knigge, Thiemo

2017-07-01

This paper describes the concept of the hyperspectral Earth-observing thermal infrared (TIR) satellite mission HiTeSEM (High-resolution Temperature and Spectral Emissivity Mapping). The scientific goal is to measure specific key variables from the biosphere, hydrosphere, pedosphere, and geosphere related to two global problems of significant societal relevance: food security and human health. The key variables comprise land and sea surface radiation temperature and emissivity, surface moisture, thermal inertia, evapotranspiration, soil minerals and grain size components, soil organic carbon, plant physiological variables, and heat fluxes. The retrieval of this information requires a TIR imaging system with adequate spatial and spectral resolutions and with day-night following observation capability. Another challenge is the monitoring of temporally high dynamic features like energy fluxes, which require adequate revisit time. The suggested solution is a sensor pointing concept to allow high revisit times for selected target regions (1-5 days at off-nadir). At the same time, global observations in the nadir direction are guaranteed with a lower temporal repeat cycle (>1 month). To account for the demand of a high spatial resolution for complex targets, it is suggested to combine in one optic (1) a hyperspectral TIR system with ~75 bands at 7.2-12.5 µm (instrument NEDT 0.05 K-0.1 K) and a ground sampling distance (GSD) of 60 m, and (2) a panchromatic high-resolution TIR-imager with two channels (8.0-10.25 µm and 10.25-12.5 µm) and a GSD of 20 m. The identified science case requires a good correlation of the instrument orbit with Sentinel-2 (maximum delay of 1-3 days) to combine data from the visible and near infrared (VNIR), the shortwave infrared (SWIR) and TIR spectral regions and to refine parameter retrieval.

The variable presentations of glycogen storage disease type IV: a review of clinical, enzymatic and molecular studies.

PubMed

Moses, Shimon W; Parvari, Ruti

2002-03-01

Glycogen storage disease type IV (GSD-IV), also known as Andersen disease or amylopectinosis (MIM 23250), is a rare autosomal recessive disorder caused by a deficiency of glycogen branching enzyme (GBE) leading to the accumulation of amylopectin-like structures in affected tissues. The disease is extremely heterogeneous in terms of tissue involvement, age of onset and clinical manifestations. The human GBE cDNA is approximately 3-kb in length and encodes a 702-amino acid protein. The GBE amino acid sequence shows a high degree of conservation throughout species. The human GBE gene is located on chromosome 3p14 and consists of 16 exons spanning at least 118 kb of chromosomal DNA. Clinically the classic Andersen disease is a rapidly progressive disorder leading to terminal liver failure unless liver transplantation is performed. Several mutations have been reported in the GBE gene in patients with classic phenotype. Mutations in the GBE gene have also been identified in patients with the milder non-progressive hepatic form of the disease. Several other variants of GSD-IV have been reported: a variant with multi-system involvement including skeletal and cardiac muscle, nerve and liver; a juvenile polysaccharidosis with multi-system involvement but normal GBE activity; and the fatal neonatal neuromuscular form associated with a splice site mutation in the GBE gene. Other presentations include cardiomyopathy, arthrogryposis and even hydrops fetalis. Polyglucosan body disease, characterized by widespread upper and lower motor neuron lesions, can present with or without GBE deficiency indicating that different biochemical defects could result in an identical phenotype. It is evident that this disease exists in multiple forms with enzymatic and molecular heterogeneity unparalleled in the other types of glycogen storage diseases.

Design and Evaluation of a Personal Diffusion Battery.

PubMed

Vosburgh, Donna J H; Klein, Timothy; Sheehan, Maura; Anthony, T Renee; Peters, Thomas M

A four-stage personal diffusion battery (pDB) was designed and constructed to measure submicron particle size distributions. The pDB consisted of a screen-type diffusion battery, solenoid valve system, and electronic controller. A data inversion spreadsheet was created to solve for the number median diameter (NMD), geometric standard deviation (GSD), and particle number concentration of unimodal aerosols using stage number concentrations from the pDB combined with a handheld condensation particle counter (pDB+CPC). The inversion spreadsheet included particle entry losses, theoretical penetrations across screens, the detection efficiency of the CPC, and constraints so the spreadsheet solved to values within the pDB range. Size distribution parameters (NMD, GSD, and number concentration) measured with the pDB+CPC with inversion spreadsheet were within 25% of those measured with a scanning mobility particle sizer (SMPS) for 5 of 12 polydisperse combustion aerosols. For three tests conducted with propylene torch exhaust, the pDB+CPC with inversion spreadsheet successfully identified that the NMD was smaller than the constraint value of 16 nm. The ratio of the nanoparticle portion of the aerosol compared to the reference ( R nano ) was calculated to determine the ability of pDB+CPC with inversion spreadsheet to measure the nanoparticle portion of the aerosols. The R nano ranged from 0.87 to 1.01 when the inversion solved and from 0.06 to 2.01 when the inversion solved to a constraint. The pDB combined with CPC has limited use as a personal monitor but combining the pDB with a different detector would allow for the pDB to be used as a personal monitor.

Design and Evaluation of a Personal Diffusion Battery

PubMed Central

Vosburgh, Donna J. H.; Klein, Timothy; Sheehan, Maura; Anthony, T. Renee; Peters, Thomas M.

2016-01-01

A four-stage personal diffusion battery (pDB) was designed and constructed to measure submicron particle size distributions. The pDB consisted of a screen-type diffusion battery, solenoid valve system, and electronic controller. A data inversion spreadsheet was created to solve for the number median diameter (NMD), geometric standard deviation (GSD), and particle number concentration of unimodal aerosols using stage number concentrations from the pDB combined with a handheld condensation particle counter (pDB+CPC). The inversion spreadsheet included particle entry losses, theoretical penetrations across screens, the detection efficiency of the CPC, and constraints so the spreadsheet solved to values within the pDB range. Size distribution parameters (NMD, GSD, and number concentration) measured with the pDB+CPC with inversion spreadsheet were within 25% of those measured with a scanning mobility particle sizer (SMPS) for 5 of 12 polydisperse combustion aerosols. For three tests conducted with propylene torch exhaust, the pDB+CPC with inversion spreadsheet successfully identified that the NMD was smaller than the constraint value of 16 nm. The ratio of the nanoparticle portion of the aerosol compared to the reference (R nano) was calculated to determine the ability of pDB+CPC with inversion spreadsheet to measure the nanoparticle portion of the aerosols. The R nano ranged from 0.87 to 1.01 when the inversion solved and from 0.06 to 2.01 when the inversion solved to a constraint. The pDB combined with CPC has limited use as a personal monitor but combining the pDB with a different detector would allow for the pDB to be used as a personal monitor. PMID:26900207

Chromosomal Context Affects the Molecular Evolution of Sex-linked Genes and Their Autosomal Counterparts in Turtles and Other Vertebrates.

PubMed

Radhakrishnan, Srihari; Valenzuela, Nicole

2017-10-30

Sex chromosomes evolve differently from autosomes because natural selection acts distinctly on them given their reduced recombination and smaller population size. Various studies of sex-linked genes compared with different autosomal genes within species support these predictions. Here, we take a novel alternative approach by comparing the rate of evolution between subsets of genes that are sex-linked in selected reptiles/vertebrates and the same genes located in autosomes in other amniotes. We report for the first time the faster evolution of Z-linked genes in a turtle (the Chinese softshell turtle Pelodiscus sinensis) relative to autosomal orthologs in other taxa, including turtles with temperature-dependent sex determination (TSD). This faster rate was absent in its close relative, the spiny softshell turtle (Apalone spinifera), thus revealing important lineage effects, and was only surpassed by mammalian-X linked genes. In contrast, we found slower evolution of X-linked genes in the musk turtle Staurotypus triporcatus (XX/XY) and homologous Z-linked chicken genes. TSD lineages displayed overall faster sequence evolution than taxa with genotypic sex determination (GSD), ruling out global effects of GSD on molecular evolution beyond those by sex-linkage. Notably, results revealed a putative selective sweep around two turtle genes involved in vertebrate gonadogenesis (Pelodiscus-Z-linked Nf2 and Chrysemys-autosomal Tspan7). Our observations reveal important evolutionary changes at the gene level mediated by chromosomal context in turtles despite their low overall evolutionary rate and illuminate sex chromosome evolution by empirically testing expectations from theoretical models. Genome-wide analyses are warranted to test the generality and prevalence of the observed patterns. © The American Genetic Association 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

The role of estrogen in turtle sex determination and the effect of PCBs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Crews, D.; Bergeron, J.M.; McLachlan, J.A.

1995-10-01

Gonadal sex is fixed at fertilization by specific chromosomes, a process known as genotypic sex determination (GSD). Only after the gonad is formed do hormones begin to exert an influence that modifies specific structures that eventually will differ between the sexes. Many egg-laying reptiles do not exhibit GSD but rather depend on the temperature of the incubating egg to determine the gonadal sex of the offspring, a process termed temperature-dependent sex determination (TSD). Research on TSD indicates that gonadal sex is not irrevocably set by the genetic composition inherited at fertilization but depends ultimately on which genes encoding for steroidogenicmore » enzymes and hormone receptors are activated during the midtrimester of embryonic development by temperature. Incubation temperature modifies the activity as well as the temporal and spatial sequence of enzymes and hormone receptors to determine gonad type. Estrogen is the physiologic equivalent of incubation temperature and the proximate cue that initiates female sex determination. increasing evidence indicates some polychlorinated biphenyl (PCB) compounds are capable of disrupting reproductive and endocrine function in fish, birds, and mammals, including humans. Reproductive disorders resulting from exposure to these xenobiotic compounds may include reductions in fertility, hatch rate in fish and birds, and viability of offspring, as well as alterations in hormone levels or adult sexual behaviors. Research on the mechanism through which these compounds may be acting to alter reproductive function indicates estrogenic activity, by which the compounds may be altering sexual differentiation. In TSD turtles, the estrogenic effect of some PCBs reverses gonadal sex in individuals incubating at an otherwise male-producing temperature. Furthermore, certain PCBs are synergistic in their effect at very low concentrations. 19 refs., 3 figs., 1 tab.« less

Transfer factors of natural radionuclides and (137)Cs from soil to plants used in traditional medicine in central Serbia.

PubMed

Djelic, Gorica; Krstic, Dragana; Stajic, Jelena M; Milenkovic, Biljana; Topuzovic, Marina; Nikezic, Dragoslav; Vucic, Dusica; Zeremski, Tijana; Stankovic, Milan; Kostic, Dragana

2016-07-01

Transfer factors of natural radionuclides and (137)Cs from soil to plants used in traditional medicine were determined. The transfer factors (TF) were calculated as Bq kg(-1) of dry plant per Bq kg(-1) of dry soil. Mass activity concentrations of (226)Ra, (232)Th, (40)K and (137)Cs in soil and plant samples were measured with high purity germanium detector (HPGe). The concentrations of As, Co, Cr, Cu, Mn, Ni, Pb and Zn were determined, as well as the cation exchange capacity (CEC) and the content of exchangeable cations (Ca, Mg, K, Na). Wide ranges of values were obtained for all the metals, especially for Cr and Ni. The Absalom model was used for determination of the amount of (137)Cs transferred from soil to plant based on soil characteristics such as pH, exchangeable potassium, humus and clay contents. The estimated transfer factors were in the range from 0.011 to 0.307 with an arithmetic mean of 0.071, median of 0.050, geometric mean of 0.053 and geometric standard deviation (GSD) of 2.08. This value agreed well with that calculated from the measurements of 0.069, geometric mean 0.040 and GSD 3.19. Correlations between radionuclides, metals, physicochemical properties and transfer factors were determined by Spearman correlation coefficient. There was a strong positive correlation between (137)Cs transfer factor and the ratio of transfer factor for K and (137)Cs. Principal Component Analysis (PCA) was performed in order to identify some pattern of data. Copyright © 2016. Published by Elsevier Ltd.

Comparison of fine particle measurements from a direct-reading instrument and a gravimetric sampling method.

PubMed

Kim, Jee Young; Magari, Shannon R; Herrick, Robert F; Smith, Thomas J; Christiani, David C

2004-11-01

Particulate air pollution, specifically the fine particle fraction (PM2.5), has been associated with increased cardiopulmonary morbidity and mortality in general population studies. Occupational exposure to fine particulate matter can exceed ambient levels by a large factor. Due to increased interest in the health effects of particulate matter, many particle sampling methods have been developed In this study, two such measurement methods were used simultaneously and compared. PM2.5 was sampled using a filter-based gravimetric sampling method and a direct-reading instrument, the TSI Inc. model 8520 DUSTTRAK aerosol monitor. Both sampling methods were used to determine the PM2.5 exposure in a group of boilermakers exposed to welding fumes and residual fuel oil ash. The geometric mean PM2.5 concentration was 0.30 mg/m3 (GSD 3.25) and 0.31 mg/m3 (GSD 2.90)from the DUSTTRAK and gravimetric method, respectively. The Spearman rank correlation coefficient for the gravimetric and DUSTTRAK PM2.5 concentrations was 0.68. Linear regression models indicated that log, DUSTTRAK PM2.5 concentrations significantly predicted loge gravimetric PM2.5 concentrations (p < 0.01). The association between log(e) DUSTTRAK and log, gravimetric PM2.5 concentrations was found to be modified by surrogate measures for seasonal variation and type of aerosol. PM2.5 measurements from the DUSTTRAK are well correlated and highly predictive of measurements from the gravimetric sampling method for the aerosols in these work environments. However, results from this study suggest that aerosol particle characteristics may affect the relationship between the gravimetric and DUSTTRAK PM2.5 measurements. Recalibration of the DUSTTRAK for the specific aerosol, as recommended by the manufacturer, may be necessary to produce valid measures of airborne particulate matter.

The French proposal for a high spatial resolution Hyperspectral mission

NASA Astrophysics Data System (ADS)

Carrère, Véronique; Briottet, Xavier; Jacquemoud, Stéphane; Marion, Rodolphe; Bourguignon, Anne; Chami, Malik; Chanussot, Jocelyn; Chevrel, Stéphane; Deliot, Philippe; Dumont, Marie; Foucher, Pierre-Yves; Gomez, Cécile; Roman-Minghelli, Audrey; Sheeren, David; Weber, Christiane; Lefèvre, Marie-José; Mandea, Mioara

2014-05-01

More than 25 years of airborne imaging spectroscopy and spaceborne sensors such as Hyperion or HICO have clearly demonstrated the ability of such a remote sensing technique to produce value added information regarding surface composition and physical properties for a large variety of applications. Scheduled missions such as EnMAP and PRISMA prove the increased interest of the scientific community for such a type of remote sensing data. In France, a group of Science and Defence users of imaging spectrometry data (Groupe de Synthèse Hyperspectral, GSH) established an up-to-date review of possible applications, define instrument specifications required for accurate, quantitative retrieval of diagnostic parameters, and identify fields of application where imaging spectrometry is a major contribution. From these conclusions, CNES (French Space Agency) decided a phase 0 study for an hyperspectral mission concept, named at this time HYPXIM (HYPerspectral-X IMagery), the main fields of applications are vegetation biodiversity, coastal and inland waters, geosciences, urban environment, atmospheric sciences, cryosphere and Defence. Results pointed out applications where high spatial resolution was necessary and would not be covered by the other foreseen hyperspectral missions. The phase A started at the beginning of 2013 based on the following HYPXIM characteristics: a hyperspectral camera covering the [0.4 - 2.5 µm] spectral range with a 8 m ground sampling distance (GSD) and a PAN camera with a 1.85 m GSD, onboard a mini-satellite platform. This phase A is currently stopped due to budget constraints. Nevertheless, the Science team is currently focusing on the preparation for the next CNES prospective meeting (March, 2014), an important step for the future of the mission. This paper will provide an update of the status of this mission and of new results obtained by the Science team.

Metabolic Response of the Cerebral Cortex Following Gentle Sleep Deprivation and Modafinil Administration

PubMed Central

Petit, Jean-Marie; Tobler, Irene; Kopp, Caroline; Morgenthaler, Florence; Borbély, Alexander A.; Magistretti, Pierre J.

2010-01-01

Study Objectives: The main energy reserve of the brain is glycogen, which is almost exclusively localized in astrocytes. We previously reported that cerebral expression of certain genes related to glycogen metabolism changed following instrumental sleep deprivation in mice. Here, we extended our investigations to another set of genes related to glycogen and glucose metabolism. We also compared the effect of instrumentally and pharmacologically induced prolonged wakefulness, followed (or not) by 3 hours of sleep recovery, on the expression of genes related to brain energy metabolism. Design: Sleep deprivation for 6–7 hours. Setting: Animal sleep research laboratory. Participants: Adults OF1 mice. Interventions: Wakefulness was maintained by “gentle sleep deprivation” method (GSD) or by administration of the wakefulness-promoting drug modafinil (MOD) (200 mg/kg i.p.). Measurements and Results: Levels of mRNAs encoding proteins related to energy metabolism were measured by quantitative real-time PCR in the cerebral cortex. The mRNAs encoding protein targeting to glycogen (PTG) and the glial glucose transporter were significantly increased following both procedures used to prolong wakefulness. Glycogenin mRNA levels were increased only after GSD, while neuronal glucose transporter mRNA only after MOD. These effects were reversed after sleep recovery. A significant enhancement of glycogen synthase activity without any changes in glycogen levels was observed in both conditions. Conclusions: These results indicate the existence of a metabolic adaptation of astrocytes aimed at maintaining brain energy homeostasis during the sleep-wake cycle. Citation: Petit, JM; Tobler I; Kopp C; Morgenthaler F; Borbély AA; Magistretti PJ. Metabolic response of the cerebral cortex following gentle sleep deprivation and modafinil administration. SLEEP 2010;33(7):901–908. PMID:20614850

Effects of coarse grain size distribution and fine particle content on pore fluid pressure and shear behavior in experimental debris flows

NASA Astrophysics Data System (ADS)

Kaitna, Roland; Palucis, Marisa C.; Yohannes, Bereket; Hill, Kimberly M.; Dietrich, William E.

2016-02-01

Debris flows are typically a saturated mixture of poorly sorted particles and interstitial fluid, whose density and flow properties depend strongly on the presence of suspended fine sediment. Recent research suggests that grain size distribution (GSD) influences excess pore pressures (i.e., pressure in excess of predicted hydrostatic pressure), which in turn plays a governing role in debris flow behaviors. We report a series of controlled laboratory experiments in a 4 m diameter vertically rotating drum where the coarse particle size distribution and the content of fine particles were varied independently. We measured basal pore fluid pressures, pore fluid pressure profiles (using novel sensor probes), velocity profiles, and longitudinal profiles of the flow height. Excess pore fluid pressure was significant for mixtures with high fines fraction. Such flows exhibited lower values for their bulk flow resistance (as measured by surface slope of the flow), had damped fluctuations of normalized fluid pressure and normal stress, and had velocity profiles where the shear was concentrated at the base of the flow. These effects were most pronounced in flows with a wide coarse GSD distribution. Sustained excess fluid pressure occurred during flow and after cessation of motion. Various mechanisms may cause dilation and contraction of the flows, and we propose that the sustained excess fluid pressures during flow and once the flow has stopped may arise from hindered particle settling and yield strength of the fluid, resulting in transfer of particle weight to the fluid. Thus, debris flow behavior may be strongly influenced by sustained excess fluid pressures controlled by particle settling rates.

Temperature-dependent sex determination in fish revisited: prevalence, a single sex ratio response pattern, and possible effects of climate change.

PubMed

Ospina-Alvarez, Natalia; Piferrer, Francesc

2008-07-30

In gonochoristic vertebrates, sex determination mechanisms can be classified as genotypic (GSD) or temperature-dependent (TSD). Some cases of TSD in fish have been questioned, but the prevalent view is that TSD is very common in this group of animals, with three different response patterns to temperature. We analyzed field and laboratory data for the 59 fish species where TSD has been explicitly or implicitly claimed so far. For each species, we compiled data on the presence or absence of sex chromosomes and determined if the sex ratio response was obtained within temperatures that the species experiences in the wild. If so, we studied whether this response was statistically significant. We found evidence that many cases of observed sex ratio shifts in response to temperature reveal thermal alterations of an otherwise predominately GSD mechanism rather than the presence of TSD. We also show that in those fish species that actually have TSD, sex ratio response to increasing temperatures invariably results in highly male-biased sex ratios, and that even small changes of just 1-2 degrees C can significantly alter the sex ratio from 1:1 (males:females) up to 3:1 in both freshwater and marine species. We demonstrate that TSD in fish is far less widespread than currently believed, suggesting that TSD is clearly the exception in fish sex determination. Further, species with TSD exhibit only one general sex ratio response pattern to temperature. However, the viability of some fish populations with TSD can be compromised through alterations in their sex ratios as a response to temperature fluctuations of the magnitude predicted by climate change.

Systemic Correction of Murine Glycogen Storage Disease Type IV by an AAV-Mediated Gene Therapy.

PubMed

Yi, Haiqing; Zhang, Quan; Brooks, Elizabeth D; Yang, Chunyu; Thurberg, Beth L; Kishnani, Priya S; Sun, Baodong

2017-03-01

Deficiency of glycogen branching enzyme (GBE) causes glycogen storage disease type IV (GSD IV), which is characterized by the accumulation of a less branched, poorly soluble form of glycogen called polyglucosan (PG) in multiple tissues. This study evaluates the efficacy of gene therapy with an adeno-associated viral (AAV) vector in a mouse model of adult form of GSD IV (Gbe1 ys/ys ). An AAV serotype 9 (AAV9) vector containing a human GBE expression cassette (AAV-GBE) was intravenously injected into 14-day-old Gbe1 ys/ys mice at a dose of 5 × 10 11 vector genomes per mouse. Mice were euthanized at 3 and 9 months of age. In the AAV-treated mice at 3 months of age, GBE enzyme activity was highly elevated in heart, which is consistent with the high copy number of the viral vector genome detected. GBE activity also increased significantly in skeletal muscles and the brain, but not in the liver. The glycogen content was reduced to wild-type levels in muscles and significantly reduced in the liver and brain. At 9 months of age, though GBE activity was only significantly elevated in the heart, glycogen levels were significantly reduced in the liver, brain, and skeletal muscles of the AAV-treated mice. In addition, the AAV treatment resulted in an overall decrease in plasma activities of alanine transaminase, aspartate transaminase, and creatine kinase, and a significant increase in fasting plasma glucose concentration at 9 months of age. This suggests an alleviation of damage and improvement of function in the liver and muscles by the AAV treatment. This study demonstrated a long-term benefit of a systemic injection of an AAV-GBE vector in Gbe1 ys/ys mice.

G6PC3 mutations are associated with a major defect of glycosylation: a novel mechanism for neutrophil dysfunction

PubMed Central

Hayee, Bu'Hussain; Antonopoulos, Aristotelis; Murphy, Emma J; Rahman, Farooq Z; Sewell, Gavin; Smith, Bradley N; McCartney, Sara; Furman, Mark; Hall, Georgina; Bloom, Stuart L; Haslam, Stuart M; Morris, Howard R; Boztug, Kaan; Klein, Christoph; Winchester, Bryan; Pick, Edgar; Linch, David C; Gale, Rosemary E; Smith, Andrew M; Dell, Anne; Segal, Anthony W

2011-01-01

Glucose-6-phosphatase, an enzyme localized in the endoplasmic reticulum (ER), catalyzes the hydrolysis of glucose-6-phosphate (G6P) to glucose and inorganic phosphate. In humans, there are three differentially expressed glucose-6-phosphatase catabolic genes (G6PC1–3). Recently, it has been shown that mutations in the G6PC3 gene result in a syndrome associating congenital neutropenia and various organ malformations. The enzymatic function of G6PC3 is dependent on G6P transport into the ER, mediated by G6P translocase (G6PT). Mutations in the gene encoding G6PT result in glycogen storage disease type-1b (GSD-1b). Interestingly, GSD-1b patients exhibit a similar neutrophil dysfunction to that observed in G6PC3-deficient patients. To better understand the causes of neutrophil dysfunction in both diseases, we have studied the neutrophil nicotinamide adenine dinucleotide phosphate (NADPH) oxidase of patients with G6PC3 and G6PT syndromes. Unexpectedly, sodium dodecyl sulfate–polyacrylamide gel electrophoresis experiments indicated hypo-glycosylation of gp91phox, the electron-transporting component of the NADPH oxidase, in all of these patients. Rigorous mass spectrometric glycomic profiling showed that most of the complex-type antennae which characterize the neutrophil N-glycome of healthy individuals were severely truncated in the patients' neutrophils. A comparable truncation of the core 2 antenna of the O-glycans was also observed. This aberrant neutrophil glycosylation is predicted to have profound effects on the neutrophil function and merit designation of both syndromes as a new class of congenital disorders of glycosylation. PMID:21385794

Healing microstructures of experimental and natural fault gouge

NASA Astrophysics Data System (ADS)

Keulen, Nynke; Stünitz, Holger; Heilbronner, RenéE.

2008-06-01

The healing of fault gouge was studied by examining microstructures of naturally and experimentally produced granitoid fault rock. We performed deformation experiments on intact granitoid rock samples at T = 300-500°C, Pc = 500 MPa, and ? = 1.2 × 10-4 - 1.3 × 10-7 s-1 with 0.2 wt% H2O added. Healing experiments were carried out on deformed samples at T = 200-500°C, Pc = 500 MPa, for 4 h to 14 days under hydrostatic and nonhydrostatic conditions. The grain size distributions (GSD) of the deformed samples were quantified using the D> value (slope of log(frequency) -log(radius) of the GSD) for quartz and feldspar fault gouge. Healing causes a decrease in the D> value from >2.0 to ˜1.5. The time dependence of the D> decrease is described by a hydrostatic healing law of the form ΔD = D>(t) - Df = A · e(-λ·t). The results of the laboratory experiments were compared to three natural fault systems, (1) Nojima Fault Zone (Japan), (2) fault zones in the Black Forest (Germany), and (3) Orobic Thrust (Italian Alps). Natural and experimental gouges have similar D> values. Healing is only observed in monomineralic aggregates; polymineralic (i.e., mixed) fault gouges retain their high D> value after extended healing times because grain growth is inhibited. Healing under nonhydrostatic conditions is more rapid than hydrostatic healing. The low strain rates, which were measured during nonhydrostatic healing, are temperature-dependent and suggest that diffusive mass transfer processes take place during deformation. Thus, fault rocks at upper to midcrustal depth may deform by combined cataclasis and diffusive mass transfer.

Combining TerraSAR-X and SPOT-5 data for object-based landslide detection

NASA Astrophysics Data System (ADS)

Friedl, B.; Hölbling, D.; Füreder, P.

2012-04-01

Landslide detection and classification is an essential requirement in pre- and post-disaster hazard analysis. In earlier studies landslide detection often was achieved through time-consuming and cost-intensive field surveys and visual orthophoto interpretation. Recent studies show that Earth Observation (EO) data offer new opportunities for fast, reliable and accurate landslide detection and classification, which may conduce to an effective landslide monitoring and landslide hazard management. To ensure the fast recognition and classification of landslides at a regional scale, a (semi-)automated object-based landslide detection approach is established for a study site situated in the Huaguoshan catchment, Southern Taiwan. The study site exhibits a high vulnerability to landslides and debris flows, which are predominantly typhoon-induced. Through the integration of optical satellite data (SPOT-5 with 2.5 m GSD), SAR (Synthetic Aperture Radar) data (TerraSAR-X Spotlight with 2.95 m GSD) and digital elevation information (DEM with 5 m GSD) including its derived products (e.g. slope, curvature, flow accumulation) landslides may be examined in a more efficient way as if relying on single data sources only. The combination of optical and SAR data in an object-based image analysis (OBIA) domain for landslide detection and classification has not been investigated so far, even if SAR imagery show valuable properties for landslide detection, which differ from optical data (e.g. high sensitivity to surface roughness and soil moisture). The main purpose of this study is to recognize and analyze existing landslides by applying object-based image analysis making use of eCognition software. OBIA provides a framework for examining features defined by spectral, spatial, textural, contextual as well as hierarchical properties. Objects are derived through image segmentation and serve as input for the classification process, which relies on transparent rulesets, representing knowledge. Through class modeling, an iterative process of segmentation and classification, objects can be addressed individually in a region-specific manner. The presented approach is marked by the comprehensive use of available data sets from various sources. This full integration of optical, SAR and DEM data conduces to the development of a robust method, which makes use of the most appropriate characteristics (e.g. spectral, textural, contextual) of each data set. The proposed method contributes to a more rapid and accurate landslide mapping in order to assist disaster and crisis management. Especially SAR data proves to be useful in the aftermath of an event, as radar sensors are mostly independent of illumination and weather conditions and therefore data is more likely to be available. The full data integration allows coming up with a robust approach for the detection and classification of landslides. However, more research is needed to make the best of the integration of SAR data in an object-based environment and for making the approach easier adaptable to different study sites and data.

Transition of NOAA's GPS-Met Data Acquisition and Processing System to the Commercial Sector: Inital Results

NASA Astrophysics Data System (ADS)

Jackson, Michael; Blatt, Stephan; Holub, Kirk

2015-04-01

In April of 2014, NOAA/OAR/ESRL Global Systems Division (GSD) and Trimble, in collaboration with Earth Networks, Inc. (ENI) signed a Cooperative Research and Development Agreement (CRADA) to transfer the existing NOAA GPS-Met Data Acquisition and Processing System (GPS-Met DAPS) technology to a commercial Trimble/ENI partnership. NOAA's GPS-Met DAPS is currently operated in a pseudo-operational mode but has proven highly reliable and running at over 95% uptime. The DAPS uses the GAMIT software to ingest dual frequency carrier phase GPS/GNSS observations and ancillary information such as real-time satellite orbits to estimate the zenith-scaled tropospheric (ZTD) signal delays and, where surface MET data are available, retrieve integrated precipitable water vapor (PWV). The NOAA data and products are made available to end users in near real-time. The Trimble/ENI partnership will use the Trimble Pivot™ software with the Atmosphere App to calculate zenith tropospheric (ZTD), tropospheric slant delay, and integrated precipitable water vapor (PWV). Evaluation of the Trimble software is underway starting with a comparison of ZTD and PWV values determined from four sub networks of GPS stations located 1. near NOAA Radiosonde Observation (Upper-Air Observation) launch sites; 2. Stations with low terrain/high moisture variability (Gulf Coast); 3. Stations with high terrain/low moisture variability (Southern California); and 4. Stations with high terrain/high moisture variability (high terrain variability elev. > 1000m). For each network GSD and T/ENI run the same stations for 30 days, compare results, and perform an evaluation of the long-term solution accuracy, precision and reliability. Metrics for success include T/ENI PWV estimates within 1.5 mm of ESRL/GSD's estimates 95% of the time (ZTD uncertainty of less than 10 mm 95% of the time). The threshold for allowable variations in ZTD between NOAA GPS-Met and T/ENI processing are 10mm. The CRADA 1&2 Trimble processing show a variation of 4±2mm and 3±8mm respectively. The threshold for allowable variations in PWV between NOAA GPS-Met and T/ENI processing are 15mm. The CRADA 1&2 Trimble processing show a variation of 2±4mm and 10±13 respectively. The T/ENI PWV and ZTD values meet and exceed the requirements outlined in the CRADA for the first two networks processed. T/ENI Partnership brings a footprint of GNSS and meteorological stations that could significantly enhance the latency, temporal, and geographic density of ZTD and PWV over the US and Europe. We will provide a brief overview of the Trimble Pivot™ software and the Atmosphere App and present results from further testing along with a timeline for the transition of the GPS-Met DAPS to an operational commercial service.

High Spatial Resolution Thermal Satellite Technologies

NASA Technical Reports Server (NTRS)

Ryan, Robert

2003-01-01

This document in the form of viewslides, reviews various low-cost alternatives to high spatial resolution thermal satellite technologies. There exists no follow-on to Landsat 7 or ASTER high spatial resolution thermal systems. This document reviews the results of the investigation in to the use of new technologies to create a low-cost useful alternative. Three suggested technologies are examined. 1. Conventional microbolometer pushbroom modes offers potential for low cost Landsat Data Continuity Mission (LDCM) thermal or ASTER capability with at least 60-120 ground sampling distance (GSD). 2. Backscanning could produce MultiSpectral Thermal Imager performance without cooled detectors. 3. Cooled detector could produce hyperspectral thermal class system or extremely high spatial resolution class instrument.

A HILIC-UHPLC-MS/MS untargeted urinary metabonomics combined with quantitative analysis of five polar biomarkers on osteoporosis rats after oral administration of Gushudan.

PubMed

Wu, Xiao; Huang, Yue; Sun, Jinghan; Wen, Yongqing; Qin, Feng; Zhao, Longshan; Xiong, Zhili

2018-01-01

A HILIC-UHPLC-MS/MS untargeted urinary metabonomic method combined with quantitative analysis of five potential polar biomarkers in rat urine was developed and validated, to further understand the anti-osteoporosis effect of Gushudan(GSD) and its mechanism on prednisolone-induced osteoporosis(OP) rats in this study. The metabolites were separated and identified on Waters BEH HILIC (2.1mm×100mm, 1.7μm) column using the Waters ACQUITY™ ultra performance liquid chromatography system (Waters Corporation, Milford, USA) coupled with a Micromass Quattro Micro™ API mass spectrometer (Waters Corp, Milford, MA, USA). Principal component analysis (PCA) was used to identify potential biomarkers. Primary potential polar biomarkers including creatinine, taurine, betaine, hypoxanthine and cytosine, which were related to energy metabolism, lipid metabolism and amino acid metabolism, were found in the untargeted metabonomic research. Moreover, these targeted biomarkers were further separated and quantified in multiple-reaction monitoring (MRM) with positive ionization mode, using tinidazole as internal standard (I.S.). Good linearities (r>0.99) were obtained for all the analytes with the low limit of quantification from 1.00 to 12.8μg/mL. The relative standard deviation (RSD) of the intra-day and inter-day precisions were within 15.0% and the accuracy ranged from -14.3% to 13.5%. The recovery was more than 85.0%. And the validated method was successfully applied to investigate the urine samples of the control group, prednisolone-induced osteoporosis model group and Gushudan-treatment group in rats. Compared to the control group, the level of creatinine, taurine, betaine, hypoxanthine and cytosine in the model group revealed a significant decrease trend (p<0.05), while the Gushudan-treatment group showed no statistically differences by an independent sample t-test. This paper provided a better understanding of the therapeutic effect and mechanism of GSD on prednisolone-induced osteoporosis rats. Copyright © 2017. Published by Elsevier B.V.

Immune interference in the setting of same-day administration of two similar inactivated alphavirus vaccines: eastern equine and western equine encephalitis.

PubMed

Reisler, Ronald B; Gibbs, Paul H; Danner, Denise K; Boudreau, Ellen F

2012-11-26

We compared the effect on primary vaccination plaque-reduction neutralization 80% titers (PRNT80) responses of same-day administration (at different injection sites) of two similar investigational inactivated alphavirus vaccines, eastern equine encephalitis (EEE) vaccine (TSI-GSD 104) and western equine encephalitis (WEE) vaccine (TSI-GSD 210) to separate administration. Overall, primary response rate for EEE vaccine was 524/796 (66%) and overall primary response rate for WEE vaccine was 291/695 (42%). EEE vaccine same-day administration yielded a 59% response rate and a responder geometric mean titer (GMT)=89 while separate administration yielded a response rate of 69% and a responder GMT=119. WEE vaccine same-day administration yielded a 30% response rate and a responder GMT=53 while separate administration yielded a response rate of 54% and a responder GMT=79. EEE response rates for same-day administration (group A) vs. non-same-day administration (group B) were significantly affected by gender. A logistic regression model predicting response to EEE comparing group B to group A for females yielded an OR=4.10 (95% CL 1.97-8.55; p=.0002) and for males yielded an OR=1.25 (95% CL 0.76-2.07; p=.3768). WEE response rates for same-day administration vs. non-same-day administration were independent of gender. A logistic regression model predicting response to WEE comparing group B to group A yielded an OR=2.14 (95% CL 1.22-3.73; p=.0077). We report immune interference occurring with same-day administration of two completely separate formalin inactivated viral vaccines in humans. These findings combined with the findings of others regarding immune interference would argue for a renewed emphasis on studying the immunological mechanisms of induction of inactivated viral vaccine protection. Copyright © 2012. Published by Elsevier Ltd.

Chemical-specific screening criteria for interpretation of biomonitoring data for volatile organic compounds (VOCs)--application of steady-state PBPK model solutions.

PubMed

Aylward, Lesa L; Kirman, Chris R; Blount, Ben C; Hays, Sean M

2010-10-01

The National Health and Nutrition Examination Survey (NHANES) generates population-representative biomonitoring data for many chemicals including volatile organic compounds (VOCs) in blood. However, no health or risk-based screening values are available to evaluate these data from a health safety perspective or to use in prioritizing among chemicals for possible risk management actions. We gathered existing risk assessment-based chronic exposure reference values such as reference doses (RfDs), reference concentrations (RfCs), tolerable daily intakes (TDIs), cancer slope factors, etc. and key pharmacokinetic model parameters for 47 VOCs. Using steady-state solutions to a generic physiologically-based pharmacokinetic (PBPK) model structure, we estimated chemical-specific steady-state venous blood concentrations across chemicals associated with unit oral and inhalation exposure rates and with chronic exposure at the identified exposure reference values. The geometric means of the slopes relating modeled steady-state blood concentrations to steady-state exposure to a unit oral dose or unit inhalation concentration among 38 compounds with available pharmacokinetic parameters were 12.0 microg/L per mg/kg-d (geometric standard deviation [GSD] of 3.2) and 3.2 microg/L per mg/m(3) (GSD=1.7), respectively. Chemical-specific blood concentration screening values based on non-cancer reference values for both oral and inhalation exposure range from 0.0005 to 100 microg/L; blood concentrations associated with cancer risk-specific doses at the 1E-05 risk level ranged from 5E-06 to 6E-02 microg/L. The distribution of modeled steady-state blood concentrations associated with unit exposure levels across VOCs may provide a basis for estimating blood concentration screening values for VOCs that lack chemical-specific pharmacokinetic data. The screening blood concentrations presented here provide a tool for risk assessment-based evaluation of population biomonitoring data for VOCs and are most appropriately applied to central tendency estimates for such datasets. Copyright (c) 2010 Elsevier Inc. All rights reserved.

Canadian population risk of radon induced lung cancer: a re-assessment based on the recent cross-Canada radon survey

PubMed Central

Chen, J.; Moir, D.; Whyte, J.

2012-01-01

Exposure to indoor radon has been determined to be the second leading cause of lung cancer after tobacco smoking. Canadian population risk of radon induced lung cancer was assessed in 2005 with the radon distribution characteristics determined from a radon survey carried out in the late 1970s in 19 cities. In that survey, a grab sampling method was used to measure radon levels. The observed radon concentration in 14 000 Canadian homes surveyed followed a log–normal distribution with a geometric mean (GM) of 11.2 Bq m–3 and a geometric standard deviation (GSD) of 3.9. Based on the information from that survey, it was estimated that ∼10 % of lung cancers in Canada resulted from indoor radon exposure. To gain a better understanding of radon concentrations in homes across the country, a national residential radon survey was launched in April 2009. In the recent survey, long-term (3 month or longer) indoor radon measurements were made in roughly 14 000 homes in 121 health regions across Canada. The observed radon concentrations follow, as expected, a log–normal distribution with a GM of 41.9 Bq m–3 and a GSD of 2.8. Based on the more accurate radon distribution characteristics obtained from the recent cross-Canada radon survey, a re-assessment of Canadian population risk for radon induced lung cancer was undertaken. The theoretical estimates show that 16 % of lung cancer deaths among Canadians are attributable to indoor radon exposure. These results strongly suggest the ongoing need for the Canadian National Radon Program. In particular, there is a need for a focus on education and awareness by all levels of government, and in partnership with key stakeholders, to encourage Canadians to take action to reduce the risk from indoor radon exposure. PMID:22874897

Variation of Soil Aggregation along the Weathering Gradient: Comparison of Grain Size Distribution under Different Disruptive Forces.

PubMed

Wei, Yujie; Wu, Xinliang; Xia, Jinwen; Shen, Xue; Cai, Chongfa

2016-01-01

The formation and stabilization of soil aggregates play a key role in soil functions. To date, few studies have been performed on the variation of soil aggregation with increasing soil weathering degree. Here, soil aggregation and its influencing factors along the weathering gradient were investigated. Six typical zonal soils (derived from similar parent materials) were sampled from temperate to tropical regions. Grain size distribution (GSD) in aggregate fragmentation with increasing disruptive forces (air-dried, water dispersion and chemical dispersion) was determined by laser diffraction particle size analyzer. Different forms of sesquioxides were determined by selective chemical extraction and their contributions to soil aggregation were identified by multiple stepwise regression analysis. The high variability of sesquioxides in different forms appeared with increasing free oxide content (Fed and Ald) from the temperate to tropical soils. The transformation of GSD peak to small size varied with increasing disruptive forces (p<0.05). Although in different weathering degrees, zonal soils showed a similar fragmentation process. Aggregate water stability generally increased with increasing soil weathering (p<0.01), with higher stability in eluvium (A) horizon than in illuvium (B) horizon (p<0.01). Crystalline oxides and amorphous iron oxides (Feo), especially (Fed-Feo) contributed to the formation of air-dried macroaggregates and their stability against slaking (R2 = 55%, p<0.01), while fine particles (<50μm) and Feo (excluding the complex form Fep) played a positive role in the formation of water stable aggregates (R2 = 93%, p<0.01). Additionally, water stable aggregates (including stability, size distribution and specific surface area) were closely related with pH, organic matter, cation exchange capacity (CEC), bulk density (BD), and free oxides (including various forms) (p<0.05). The overall results indicate that soil aggregation conforms to aggregate hierarchy theory to some extent along the weathering gradient and different forms of sesquioxides perform their specific roles in the formation and stabilization of different size aggregates.

Assessment of a web-based Guided Self-Determination intervention for adults with type 2 diabetes in general practice: a study protocol

PubMed Central

Karlsen, Bjørg; Oftedal, Bjørg; Stangeland Lie, Silje; Rokne, Berit; Peyrot, Mark; Zoffmann, Vibeke; Graue, Marit

2016-01-01

Introduction Self-management is deemed the cornerstone in overall diabetes management. Web-based self-management interventions have potential to support adults with type 2 diabetes (T2DM) in managing their disease. Owing to somewhat ambiguous results of such interventions, interventions should be theory-based and incorporate well-defined counselling methods and techniques for behavioural change. This study is designed to assess the effectiveness of a theory-driven web-based Guided Self-Determination (GSD) intervention among adults with T2DM in general practice to improve diabetes self-management behaviours and glycosylated haemoglobin (HbA1c). Methods and analysis A complex intervention design based on the framework of the UK Medical Research Council is employed as a guide for developing the intervention, assessing its feasibility and evaluating its effectiveness. The study consists of three phases: (1) the modelling phase adapting the original GSD programme for adults with T2DM, using a qualitative design, (2) feasibility assessment of the adapted intervention on the web, employing qualitative and quantitative methods and (3) evaluating the effectiveness of the intervention on diabetes self-management behaviours and HbA1c, using a quasi-experimental design. The first phase, which is completed, and the second phase, which is underway, will provide important information about the development of the intervention and its acceptability, whereas the third phase will assess the effectiveness of this systematically developed intervention. Ethics and dissemination The Norwegian Regional Committee for Medical and Health Research Ethics (REK west number 2015/60) has approved the study design. Patients recruited in the different phases will fill out an informed consent form prior to inclusion and will be guaranteed anonymity and the right to withdraw from the study at any time. The results of the study will be published in peer-reviewed journals, electronically and in print, and presented at research conferences. Trial registration number: NCT02575599. PMID:27965253

Expression profiles of amhy and major sex-related genes during gonadal sex differentiation and their relation with genotypic and temperature-dependent sex determination in pejerrey Odontesthes bonariensis.

PubMed

Zhang, Yan; Hattori, Ricardo S; Sarida, Munti; García, Estefany L; Strüssmann, Carlos Augusto; Yamamoto, Yoji

2018-03-15

To shed light on the mechanisms of and interactions of GSD and TSD in pejerrey, we investigated how the transcriptional profiles of amhy and amha are affected by feminizing (17 °C) and masculinizing (29 °C) temperatures during the critical period of sex determination/differentiation and their relation with the expression profiles of AMH receptor type II (amhrII), gonadal aromatase (cyp19a1a), and 11 beta-hydroxysteroid dehydrogenase 2 (hsd11b2). Careful consideration of the results of this study and all information currently available for this species, including similar analyzes for an intermediate, mixed-sex promoting temperature (25 °C), suggests a model for genotypic/temperature-dependent sex determination and gonadal sex differentiation that involves a) cyp19a1a-dependent, developmentally-programmed ovarian development as the default state that becomes self-sustaining in the absence of a potent and timely masculinizing stimulus, b) early, developmentally-programmed amhy expression and high temperature as masculinization signals that antagonize the putative female pathway by suppressing cyp19a1a expression, c) increasing stress response, cortisol, and the synthesis of the masculinizing androgen 11-keto-testosterone via hsd11b2 with increasing temperature that is important for masculinization in both genotypes but particularly so in XX individuals, and d) an endocrine network with positive/negative feedback mechanisms that ensure fidelity of the male/female pathway once started. The proposed model, albeit tentative and non-all inclusive, accounts for the continuum of responses, from all-females at low temperatures to all-males at high temperatures and for the balanced-, genotype-linked sex ratios obtained at intermediate temperatures, and therefore supports the coexistence of TSD and GSD in pejerrey across the range of viable temperatures for this species. Copyright © 2018 Elsevier Inc. All rights reserved.

Downregulation of SIRT1 signaling underlies hepatic autophagy impairment in glycogen storage disease type Ia

PubMed Central

Cho, Jun-Ho; Pan, Chi-Jiunn; Anduaga, Javier

2017-01-01

A deficiency in glucose-6-phosphatase-α (G6Pase-α) in glycogen storage disease type Ia (GSD-Ia) leads to impaired glucose homeostasis and metabolic manifestations including hepatomegaly caused by increased glycogen and neutral fat accumulation. A recent report showed that G6Pase-α deficiency causes impairment in autophagy, a recycling process important for cellular metabolism. However, the molecular mechanism underlying defective autophagy is unclear. Here we show that in mice, liver-specific knockout of G6Pase-α (L-G6pc-/-) leads to downregulation of sirtuin 1 (SIRT1) signaling that activates autophagy via deacetylation of autophagy-related (ATG) proteins and forkhead box O (FoxO) family of transcriptional factors which transactivate autophagy genes. Consistently, defective autophagy in G6Pase-α-deficient liver is characterized by attenuated expressions of autophagy components, increased acetylation of ATG5 and ATG7, decreased conjugation of ATG5 and ATG12, and reduced autophagic flux. We further show that hepatic G6Pase-α deficiency results in activation of carbohydrate response element-binding protein, a lipogenic transcription factor, increased expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), a lipid regulator, and suppressed expression of PPAR-α, a master regulator of fatty acid β-oxidation, all contributing to hepatic steatosis and downregulation of SIRT1 expression. An adenovirus vector-mediated increase in hepatic SIRT1 expression corrects autophagy defects but does not rectify metabolic abnormalities associated with G6Pase-α deficiency. Importantly, a recombinant adeno-associated virus (rAAV) vector-mediated restoration of hepatic G6Pase-α expression corrects metabolic abnormalities, restores SIRT1-FoxO signaling, and normalizes defective autophagy. Taken together, these data show that hepatic G6Pase-α deficiency-mediated down-regulation of SIRT1 signaling underlies defective hepatic autophagy in GSD-Ia. PMID:28558013

Mutual information registration of multi-spectral and multi-resolution images of DigitalGlobe's WorldView-3 imaging satellite

NASA Astrophysics Data System (ADS)

Miecznik, Grzegorz; Shafer, Jeff; Baugh, William M.; Bader, Brett; Karspeck, Milan; Pacifici, Fabio

2017-05-01

WorldView-3 (WV-3) is a DigitalGlobe commercial, high resolution, push-broom imaging satellite with three instruments: visible and near-infrared VNIR consisting of panchromatic (0.3m nadir GSD) plus multi-spectral (1.2m), short-wave infrared SWIR (3.7m), and multi-spectral CAVIS (30m). Nine VNIR bands, which are on one instrument, are nearly perfectly registered to each other, whereas eight SWIR bands, belonging to the second instrument, are misaligned with respect to VNIR and to each other. Geometric calibration and ortho-rectification results in a VNIR/SWIR alignment which is accurate to approximately 0.75 SWIR pixel at 3.7m GSD, whereas inter-SWIR, band to band registration is 0.3 SWIR pixel. Numerous high resolution, spectral applications, such as object classification and material identification, require more accurate registration, which can be achieved by utilizing image processing algorithms, for example Mutual Information (MI). Although MI-based co-registration algorithms are highly accurate, implementation details for automated processing can be challenging. One particular challenge is how to compute bin widths of intensity histograms, which are fundamental building blocks of MI. We solve this problem by making the bin widths proportional to instrument shot noise. Next, we show how to take advantage of multiple VNIR bands, and improve registration sensitivity to image alignment. To meet this goal, we employ Canonical Correlation Analysis, which maximizes VNIR/SWIR correlation through an optimal linear combination of VNIR bands. Finally we explore how to register images corresponding to different spatial resolutions. We show that MI computed at a low-resolution grid is more sensitive to alignment parameters than MI computed at a high-resolution grid. The proposed modifications allow us to improve VNIR/SWIR registration to better than ¼ of a SWIR pixel, as long as terrain elevation is properly accounted for, and clouds and water are masked out.

Dust exposure in workers from grain storage facilities in Costa Rica.

PubMed

Rodríguez-Zamora, María G; Medina-Escobar, Lourdes; Mora, Glend; Zock, Jan-Paul; van Wendel de Joode, Berna; Mora, Ana M

2017-08-01

About 12 million workers are involved in the production of basic grains in Central America. However, few studies in the region have examined the occupational factors associated with inhalable dust exposure. (i) To assess the exposure to inhalable dust in workers from rice, maize, and wheat storage facilities in Costa Rica; (ii) to examine the occupational factors associated with this exposure; and (iii) to measure concentrations of respirable and thoracic particles in different areas of the storage facilities. We measured inhalable (<100μm) dust concentrations in 176 personal samples collected from 136 workers of eight grain storage facilities in Costa Rica. We also measured respirable (<4μm) and thoracic (<10μm) dust particles in several areas of the storage facilities. Geometric mean (GM) and geometric standard deviation (GSD) inhalable dust concentrations were 2.0mg/m 3 and 7.8 (range=<0.2-275.4mg/m 3 ). Personal inhalable dust concentrations were associated with job category [GM for category/GM for administrative staff and other workers (95% CI)=4.4 (2.6, 7.2) for packing; 20.4 (12.3, 34.7) for dehulling; 109.6 (50.1, 234.4) for unloading in flat bed sheds; 24.0 (14.5, 39.8) for unloading in pits; and 31.6 (18.6, 52.5) for drying], and cleaning task [15.8 (95% CI: 10.0, 26.3) in workers who cleaned in addition to their regular tasks]. Higher area concentrations of thoracic dust particles were found in wheat (GM and GSD=4.3mg/m 3 and 4.5) and maize (3.0mg/m 3 and 3.9) storage facilities, and in grain drying (2.3mg/m 3 and 3.1) and unloading (1.5mg/m 3 and 4.8) areas. Operators of grain storage facilities showed elevated inhalable dust concentrations, mostly above international exposure limits. Better engineering and administrative controls are needed. Copyright © 2017 Elsevier GmbH. All rights reserved.

The ecology and evolution of temperature-dependent reaction norms for sex determination in reptiles: a mechanistic conceptual model.

PubMed

Pezaro, Nadav; Doody, J Sean; Thompson, Michael B

2017-08-01

Sex-determining mechanisms are broadly categorised as being based on either genetic or environmental factors. Vertebrate sex determination exhibits remarkable diversity but displays distinct phylogenetic patterns. While all eutherian mammals possess XY male heterogamety and female heterogamety (ZW) is ubiquitous in birds, poikilothermic vertebrates (fish, amphibians and reptiles) exhibit multiple genetic sex-determination (GSD) systems as well as environmental sex determination (ESD). Temperature is the factor controlling ESD in reptiles and temperature-dependent sex determination (TSD) in reptiles has become a focal point in the study of this phenomenon. Current patterns of climate change may cause detrimental skews in the population sex ratios of reptiles exhibiting TSD. Understanding the patterns of variation, both within and among populations and linking such patterns with the selection processes they are associated with, is the central challenge of research aimed at predicting the capacity of populations to adapt to novel conditions. Here we present a conceptual model that innovates by defining an individual reaction norm for sex determination as a range of incubation temperatures. By deconstructing individual reaction norms for TSD and revealing their underlying interacting elements, we offer a conceptual solution that explains how variation among individual reaction norms can be inferred from the pattern of population reaction norms. The model also links environmental variation with the different patterns of TSD and describes the processes from which they may arise. Specific climate scenarios are singled out as eco-evolutionary traps that may lead to demographic extinction or a transition to either male or female heterogametic GSD. We describe how the conceptual principles can be applied to interpret TSD data and to explain the adaptive capacity of TSD to climate change as well as its limits and the potential applications for conservation and management programs. © 2016 Cambridge Philosophical Society.

A Satellite-Based Imaging Instrumentation Concept for Hyperspectral Thermal Remote Sensing

PubMed Central

Udelhoven, Thomas; Schlerf, Martin; Segl, Karl; Mallick, Kaniska; Bossung, Christian; Rock, Gilles; Fischer, Peter; Müller, Andreas; Storch, Tobias; Eisele, Andreas; Weise, Dennis; Hupfer, Werner; Knigge, Thiemo

2017-01-01

This paper describes the concept of the hyperspectral Earth-observing thermal infrared (TIR) satellite mission HiTeSEM (High-resolution Temperature and Spectral Emissivity Mapping). The scientific goal is to measure specific key variables from the biosphere, hydrosphere, pedosphere, and geosphere related to two global problems of significant societal relevance: food security and human health. The key variables comprise land and sea surface radiation temperature and emissivity, surface moisture, thermal inertia, evapotranspiration, soil minerals and grain size components, soil organic carbon, plant physiological variables, and heat fluxes. The retrieval of this information requires a TIR imaging system with adequate spatial and spectral resolutions and with day-night following observation capability. Another challenge is the monitoring of temporally high dynamic features like energy fluxes, which require adequate revisit time. The suggested solution is a sensor pointing concept to allow high revisit times for selected target regions (1–5 days at off-nadir). At the same time, global observations in the nadir direction are guaranteed with a lower temporal repeat cycle (>1 month). To account for the demand of a high spatial resolution for complex targets, it is suggested to combine in one optic (1) a hyperspectral TIR system with ~75 bands at 7.2–12.5 µm (instrument NEDT 0.05 K–0.1 K) and a ground sampling distance (GSD) of 60 m, and (2) a panchromatic high-resolution TIR-imager with two channels (8.0–10.25 µm and 10.25–12.5 µm) and a GSD of 20 m. The identified science case requires a good correlation of the instrument orbit with Sentinel-2 (maximum delay of 1–3 days) to combine data from the visible and near infrared (VNIR), the shortwave infrared (SWIR) and TIR spectral regions and to refine parameter retrieval. PMID:28671575

Transition of NOAA's GPS-Met Data Acquisition and Processing System to the Commercial Sector

NASA Astrophysics Data System (ADS)

Jackson, M. E.; Holub, K.; Callahan, W.; Blatt, S.

2014-12-01

In April of 2014, NOAA/OAR/ESRL Global Systems Division (GSD) and Trimble, in collaboration with Earth Networks, Inc. (ENI) signed a Cooperative Research and Development Agreement (CRADA) to transfer the existing NOAA GPS-Met Data Acquisition and Processing System (GPS-Met DAPS) technology to a commercial Trimble/ENI partnership. NOAA's GPS-Met DAPS is currently operated in a pseudo-operational mode but has proven highly reliable and running at over 95% uptime. The DAPS uses the GAMIT software to ingest dual frequency carrier phase GPS/GNSS observations and ancillary information such as real-time satellite orbits to estimate the zenith-scaled tropospheric (ZTD) signal delays and, where surface MET data are available, retrieve integrated precipitable water vapor (PWV). The NOAA data and products are made available to end users in near real-time. The Trimble/ENI partnership will use the Trimble Pivot™ software with the Atmosphere App to calculate zenith tropospheric (ZTD), tropospheric slant delay, and integrated precipitable water vapor (PWV). Evaluation of the Trimble software is underway starting with a comparison of ZTD and PWV values determined from GPS stations located near NOAA Radiosonde Observation (Upper-Air Observation) launch sites. A success metric was established that requires Trimble's PWV estimates to match ESRL/GSD's to within 1.5 mm 95% of the time, which corresponds to a ZTD uncertainty of less than 10 mm 95% of the time. Initial results indicate that Trimble/ENI data meet and exceed the ZTD metric, but for some stations PWV estimates are out of specification. These discrepancies are primarily due to how offsets between MET and GPS stations are handled and are easily resolved. Additional test networks are proposed that include low terrain/high moisture variability stations, high terrain/low moisture variability stations, as well as high terrain/high moisture variability stations. We will present results from further testing along with a timeline for the transition of the GPS-Met DAPS to an operational commercial service.

Preliminary characterisation of new glass reference materials (GSA-1G, GSC-1G, GSD-1G and GSE-1G) by laser ablation-inductively coupled plasma-mass spectrometry using 193 nm, 213 nm and 266 nm wavelengths

USGS Publications Warehouse

Guillong, M.; Hametner, K.; Reusser, E.; Wilson, S.A.; Gunther, D.

2005-01-01

New glass reference materials GSA-1G, GSC-1G, GSD-1G and GSE-1G have been characterised using a prototype solid state laser ablation system capable of producing wavelengths of 193 nm, 213 nm and 266 nm. This system allowed comparison of the effects of different laser wavelengths under nearly identical ablation and ICP operating conditions. The wavelengths 213 nm and 266 nm were also used at higher energy densities to evaluate the influence of energy density on quantitative analysis. In addition, the glass reference materials were analysed using commercially available 266 nm Nd:YAG and 193 nm ArF excimer lasers. Laser ablation analysis was carried out using both single spot and scanning mode ablation. Using laser ablation ICP-MS, concentrations of fifty-eight elements were determined with external calibration to the NIST SRM 610 glass reference material. Instead of applying the more common internal standardisation procedure, the total concentration of all element oxide concentrations was normalised to 100%. Major element concentrations were compared with those determined by electron microprobe. In addition to NIST SRM 610 for external calibration, USGS BCR-2G was used as a more closely matrix-matched reference material in order to compare the effect of matrix-matched and non matrix-matched calibration on quantitative analysis. The results show that the various laser wavelengths and energy densities applied produced similar results, with the exception of scanning mode ablation at 266 nm without matrix-matched calibration where deviations up to 60% from the average were found. However, results acquired using a scanning mode with a matrix-matched calibration agreed with results obtained by spot analysis. The increased abundance of large particles produced when using a scanning ablation mode with NIST SRM 610, is responsible for elemental fractionation effects caused by incomplete vaporisation of large particles in the ICP.

GAMBLINGLESS: FOR LIFE study protocol: a pragmatic randomised trial of an online cognitive–behavioural programme for disordered gambling

PubMed Central

Merkouris, S S; Rodda, S N; Austin, D; Lubman, D I; Harvey, P; Battersby, M; Cunningham, J; Lavis, T; Smith, D; Dowling, N A

2017-01-01

Introduction The prevalence of disordered gambling worldwide has been estimated at 2.3%. Only a small minority of disordered gamblers seek specialist face-to-face treatment, and so a need for alternative treatment delivery models that capitalise on advances in communication technology, and use self-directed activity that can complement existing services has been identified. As such, the primary aim of this study is to evaluate an online self-directed cognitive–behavioural programme for disordered gambling (GamblingLess: For Life). Methods and analysis The study will be a 2-arm, parallel group, pragmatic randomised trial. Participants will be randomly allocated to a pure self-directed (PSD) or guided self-directed (GSD) intervention. Participants in both groups will be asked to work through the 4 modules of the GamblingLess programme over 8 weeks. Participants in the GSD intervention will also receive weekly emails of guidance and support from a gambling counsellor. A total of 200 participants will be recruited. Participants will be eligible if they reside in Australia, are aged 18 years and over, have access to the internet, have adequate knowledge of the English language, are seeking help for their own gambling problems and are willing to take part in the intervention and associated assessments. Assessments will be conducted at preintervention, and at 2, 3 and 12 months from preintervention. The primary outcome is gambling severity, assessed using the Gambling Symptom Assessment Scale. Secondary outcomes include gambling frequency, gambling expenditure, psychological distress, quality of life and additional help-seeking. Qualitative interviews will also be conducted with a subsample of participants and the Guides (counsellors). Ethics and dissemination The study has been approved by the Deakin University Human Research and Eastern Health Human Research Ethics Committees. Findings will be disseminated via report, peer-reviewed publications and conference presentations. Trial registration number ACTRN12615000864527; results. PMID:28235970

Variation of Soil Aggregation along the Weathering Gradient: Comparison of Grain Size Distribution under Different Disruptive Forces

PubMed Central

Wu, Xinliang; Xia, Jinwen; Shen, Xue; Cai, Chongfa

2016-01-01

The formation and stabilization of soil aggregates play a key role in soil functions. To date, few studies have been performed on the variation of soil aggregation with increasing soil weathering degree. Here, soil aggregation and its influencing factors along the weathering gradient were investigated. Six typical zonal soils (derived from similar parent materials) were sampled from temperate to tropical regions. Grain size distribution (GSD) in aggregate fragmentation with increasing disruptive forces (air-dried, water dispersion and chemical dispersion) was determined by laser diffraction particle size analyzer. Different forms of sesquioxides were determined by selective chemical extraction and their contributions to soil aggregation were identified by multiple stepwise regression analysis. The high variability of sesquioxides in different forms appeared with increasing free oxide content (Fed and Ald) from the temperate to tropical soils. The transformation of GSD peak to small size varied with increasing disruptive forces (p<0.05). Although in different weathering degrees, zonal soils showed a similar fragmentation process. Aggregate water stability generally increased with increasing soil weathering (p<0.01), with higher stability in eluvium (A) horizon than in illuvium (B) horizon (p<0.01). Crystalline oxides and amorphous iron oxides (Feo), especially (Fed-Feo) contributed to the formation of air-dried macroaggregates and their stability against slaking (R2 = 55%, p<0.01), while fine particles (<50μm) and Feo (excluding the complex form Fep) played a positive role in the formation of water stable aggregates (R2 = 93%, p<0.01). Additionally, water stable aggregates (including stability, size distribution and specific surface area) were closely related with pH, organic matter, cation exchange capacity (CEC), bulk density (BD), and free oxides (including various forms) (p<0.05). The overall results indicate that soil aggregation conforms to aggregate hierarchy theory to some extent along the weathering gradient and different forms of sesquioxides perform their specific roles in the formation and stabilization of different size aggregates. PMID:27529618

X-cube model on generic lattices: Fracton phases and geometric order

NASA Astrophysics Data System (ADS)

Slagle, Kevin; Kim, Yong Baek

2018-04-01

Fracton order is a new kind of quantum order characterized by topological excitations that exhibit remarkable mobility restrictions and a robust ground-state degeneracy (GSD) which can increase exponentially with system size. In this paper, we present a generic lattice construction (in three dimensions) for a generalized X-cube model of fracton order, where the mobility restrictions of the subdimensional particles inherit the geometry of the lattice. This helps explain a previous result that lattice curvature can produce a robust GSD, even on a manifold with trivial topology. We provide explicit examples to show that the (zero-temperature) phase of matter is sensitive to the lattice geometry. In one example, the lattice geometry confines the dimension-1 particles to small loops, which allows the fractons to be fully mobile charges, and the resulting phase is equivalent to (3+1)-dimensional toric code. However, the phase is sensitive to more than just lattice curvature; different lattices without curvature (e.g., cubic or stacked kagome lattices) also result in different phases of matter, which are separated by phase transitions. Unintuitively, however, according to a previous definition of phase [X. Chen et al., Phys. Rev. B 82, 155138 (2010), 10.1103/PhysRevB.82.155138], even just a rotated or rescaled cubic results in different phases of matter, which motivates us to propose a coarser definition of phase for gapped ground states and fracton order. This equivalence relation between ground states is given by the composition of a local unitary transformation and a quasi-isometry (which can rotate and rescale the lattice); equivalently, ground states are in the same phase if they can be adiabatically connected by varying both the Hamiltonian and the positions of the degrees of freedom (via a quasi-isometry). In light of the importance of geometry, we further propose that fracton orders should be regarded as a geometric order.

WorldView-2 and the evolution of the DigitalGlobe remote sensing satellite constellation: introductory paper for the special session on WorldView-2

NASA Astrophysics Data System (ADS)

Anderson, Neal T.; Marchisio, Giovanni B.

2012-06-01

Over the last decade DigitalGlobe (DG) has built and launched a series of remote sensing satellites with steadily increasing capabilities: QuickBird, WorldView-1 (WV-1), and WorldView-2 (WV-2). Today, this constellation acquires over 2.5 million km2 of imagery on a daily basis. This paper presents the configuration and performance capabilities of each of these satellites, with emphasis on the unique spatial and spectral capabilities of WV-2. WV-2 employs high-precision star tracker and inertial measurement units to achieve a geolocation accuracy of 5 m Circular Error, 90% confidence (CE90). The native resolution of WV-2 is 0.5 m GSD in the panchromatic band and 2 m GSD in 8 multispectral bands. Four of the multispectral bands match those of the Landsat series of satellites; four new bands enable novel and expanded applications. We are rapidly establishing and refreshing a global database of very high resolution (VHR) 8-band multispectral imagery. Control moment gyroscopes (CMGs) on both WV-1 and WV-2 improve collection capacity and provide the agility to capture multi-angle sequences in rapid succession. These capabilities result in a rich combination of image features that can be exploited to develop enhanced monitoring solutions. Algorithms for interpretation and analysis can leverage: 1) broader and more continuous spectral coverage at 2 m resolution; 2) textural and morphological information from the 0.5 m panchromatic band; 3) ancillary information from stereo and multi-angle collects, including high precision digital elevation models; 4) frequent revisits and time-series collects; and 5) the global reference image archives. We introduce the topic of creative fusion of image attributes, as this provides a unifying theme for many of the papers in this WV-2 Special Session.

A comparison of multicopter and fixed-wing unmanned aerial systems (UAS) applied to mapping debris flows in small alpine catchments

NASA Astrophysics Data System (ADS)

Sotier, Bernadette; Lechner, Veronika

2016-04-01

The use of unmanned aerial systems (UAS) for documenting natural hazard events (e.g. debris flows) is becoming increasingly popular, as UAS allow on-demand, flexible and cost-efficient data acquisition. In this paper, we present the results of a comparison of multicopter and fixed-wing UAS. They were employed in the summer of 2015 to map two small alpine catchments located in Western Austria, where debris flows had occurred recently: The first event took place in the Seigesbach (Tyrol), the second occurred in the Plojergraben (Salzburg). For the Seigesbach mission, a fixed-wing UAS (Multiplex Mentor), equipped with a Sony NEX5 (50 mm prime lens, 14 MP sensor resolution) was employed to acquire approximately 4,000 images. In the Plojergraben an AustroDrones X18 octocopter was used, carrying a Sony ILCE-7R (35 mm prime lens, 36 MP sensor resolution) to record 1,700 images. Both sites had a size of approximately 2km². 20 ground control points (GCP) were distributed within both catchments, and their location was measured (Trimble GeoXT, expected accuracy 0.15 m). Using standard structure-from-motion photogrammetry software (AgiSoft PhotoScan Pro, v. 1.1.6), orthophotos (5 cm ground sampling distance - GSD) and digital surface models (DSM) (20 cm GSD) were calculated. Volume differences caused by the debris flow (i.e. deposition heights and erosion depths) computed by subtracting post-event from pre-event DSMs. Even though the terrain conditions in the two catchments were comparable, the challenges during the field campaign and the evaluation of the aerial images were very different. The main difference between the two campaigns was the number of flights required to cover the catchment: only four were needed by the fixed-wing UAS, while the multicopter required eleven in the Plojergraben. The fixed-wing UAS is specially designed for missions in hardly accessible regions, requiring only two people to carry the whole equipment, while in this case a car was needed for the multicopter deployment. This plays an important role especially for the monitoring of events, where the access roads were destroyed or non-existent. On the other hand, the fixed-wing UAS requires more space for starting and landing. Both campaigns were performed over a full day therefore the lighting conditions changed from flight to flight, affecting the quality of the recorded images. Although the Sony ILCE-7R offers much higher images quality and higher sensor resolution, the results of image processing of Seigesbach and Plojergraben are comparable in terms of processing time, GSD and accuracy for this application. One important difference between the campaigns is for example, that in the Plojergraben, the torrent is partly hidden by bank-side trees and many trees are lying in the riverbed, which causes large errors in calculated volumes. From our experience, external conditions like lighting, visibility and accessibility are determining factors for getting high-quality results in alpine environments, and good results are possible with low-cost equipment. Notwithstanding the operational constraints, the choice of the platform therefore is of secondary importance for debris flow volume mapping.

Perl at the Joint Astronomy Centre

NASA Astrophysics Data System (ADS)

Jenness, Tim; Economou, Frossie; Tilanus, Remo P. J.; Best, Casey; Prestage, Richard M.; Shimek, Pam; Glazebrook, Karl; Farrell, Tony J.

Perl is used extensively at the JAC (UKIRT and JCMT) and because of the language's flexibility (enabling us to interface perl to any library) we are finding that it is possible to write all of our utilities in it. This simplifies support and aids code reuse (via the module system and object oriented interface) as well as shortening development time. Currently we have developed interfaces to messaging systems (ADAM and DRAMA), I/O libraries (NDF, GSD), astronomical libraries (SLALIB) and the Starlink noticeboard system (NBS). We have also developed tools to aid in data taking (the JCMT observation desk) and data processing (surf and orac-dr) This paper will briefly review the facilities available, with an emphasis on those which might be of interest to other observatories.

Nondestructive evaluation of degradation in papaya fruit using intensity based algorithms

NASA Astrophysics Data System (ADS)

Kumari, Shubhashri; Nirala, Anil Kumar

2018-05-01

In the proposed work degradation in Papaya fruit has been evaluated nondestructively using laser biospeckle technique. The biospeckle activity inside the fruit has been evaluated qualitatively and quantitatively during its maturity to degradation stage using intensity based algorithms. Co-occurrence matrix (COM) has been used for qualitative analysis whereas Inertia Moment (IM), Absolute value Difference (AVD) and Autocovariance methods have been used for quantitative analysis. The biospeckle activity has been found to first increase and then decrease during study period of five days. In addition Granulometric size distribution (GSD) has also been used for the first time for the evaluation of degradation of the papaya. It is concluded that the degradation process of papaya fruit can be evaluated nondestructively using all the mentioned algorithms.

Hydrologic data and groundwater flow simulations in the vicinity of Long Lake, Indiana Dunes National Lakeshore, near Gary, Indiana

USGS Publications Warehouse

Lampe, David C.; Bayless, E. Randall

2013-01-01

The U.S. Geological Survey (USGS) collected data and simulated groundwater flow to increase understanding of the hydrology and the effects of drainage alterations to the water table in the vicinity of Long Lake, near Gary, Indiana. East Long Lake and West Long Lake (collectively known as Long Lake) make up one of the largest interdunal lakes within the Indiana Dunes National Lakeshore. The National Park Service is tasked with preservation and restoration of wetlands in the Indiana Dunes National Lakeshore along the southern shoreline of Lake Michigan. Urban development and engineering have modified drainage and caused changes in the distribution of open water, streams and ditches, and groundwater abundance and flow paths. A better understanding of the effects these modifications have on the hydrologic system in the area will help the National Park Service, the Gary Sanitary District (GSD), and local stakeholders manage and protect the resources within the study area.This study used hydrologic data and steady-state groundwater simulations to estimate directions of groundwater flow and the effects of various engineering controls and climatic conditions on the hydrology near Long Lake. Periods of relatively high and low groundwater levels were examined and simulated by using MODFLOW and companion software. Simulated hydrologic modifications examined the effects of (1) removing the beaver dams in US-12 ditch, (2) discontinuing seepage of water from the filtration pond east of East Long Lake, (3) discontinuing discharge from US-12 ditch to the GSD sewer system, (4) decreasing discharge from US-12 ditch to the GSD sewer system, (5) connecting East Long Lake and West Long Lake, (6) deepening County Line Road ditch, and (7) raising and lowering the water level of Lake Michigan.Results from collected hydrologic data indicate that East Long Lake functioned as an area of groundwater recharge during October 2002 and a “flow-through” lake during March 2011, with the groundwater divide south of US-12. Wetlands to the south of West Long Lake act as points of recharge to the surficial aquifer in both dry- and wet-weather conditions.Among the noteworthy results from a dry-weather groundwater flow model simulation are (1) US-12 ditch does not receive water from East Long Lake or West Long Lake, (2) the filtration pond at the east end of East Long Lake, when active, contributed approximately 10 percent of the total water entering East Long Lake, and (3) County Line Road ditch has little effect on simulated water level.Among the noteworthy results from a wet-weather groundwater flow simulation are (1) US-12 ditch does not receive water from East Long Lake or West Long Lake, (2) when the seepage from the filtration pond to the surficial aquifer is not active, sources of inflow to East Long Lake are restricted to only precipitation (46 percent of total) and inflow from the surficial aquifer (54 percent of total), and (3) County Line Road ditch bisects the groundwater divide and creates two water-table mounds south of US-12.The results from a series of model scenarios simulating certain engineering controls and changes in Lake Michigan levels include the following: (1) The simulated removal of beaver dams in US-12 ditch during a wet-weather simulation increased discharge from the ditch to the Gary Sanitary system by 13 percent. (2) Discontinuation of seepage from the filtration pond east of East Long Lake decreased discharge from US-12 ditch to the Gary Sanitary system by 2.3 percent. (3) Simulated discontinuation of discharge from the US-12 ditch to the GSD sewer system increased the area where the water table was estimated to be above the land surface beyond the inundated area in the initial wet-weather simulation. (4) Simulated modifications to the control structure at the discharge point of US-12 ditch to the GSD sewer system can decrease discharge by as much as 61 percent while increasing the simulated inundated area during dry weather and decrease discharge as much as 6 percent while increasing the simulated inundated area during wet weather. (5) Deepening of County Line Road ditch can decrease the discharge from US-12 ditch by 26 percent during dry weather and 24 percent during wet weather, as well as decrease the extent of flooded areas south and east of the filtration pond near Ogden Dunes. (7) The increase of the Lake Michigan water level to match the historical maximum can increase the discharge from US-12 ditch by 14 percent during dry weather and by 9.6 percent during wet weather. (8) The decrease of the Lake Michigan water level to match the historical minimum can decrease the discharge from US-12 ditch by 7.4 percent during dry weather and by 3.1 percent during wet weather.The results of this study can be used by water-resource managers to understand how surrounding ditches affect water levels in East and West Long Lake and in the surrounding wetlands and residential areas. The groundwater model developed in this study can be applied in the future to answer questions about how alterations to the drainage system in the area will affect water levels in East and West Long Lake and surrounding areas. The modeling methods developed in this study provide a template for other studies of groundwater flow and groundwater/surface-water interactions within the shallow surficial aquifer in northern Indiana, and in similar hydrologic settings that include surficial sand aquifers in coastal settings.

A Comparison of the β-Substitution Method and a Bayesian Method for Analyzing Left-Censored Data

PubMed Central

Huynh, Tran; Quick, Harrison; Ramachandran, Gurumurthy; Banerjee, Sudipto; Stenzel, Mark; Sandler, Dale P.; Engel, Lawrence S.; Kwok, Richard K.; Blair, Aaron; Stewart, Patricia A.

2016-01-01

Classical statistical methods for analyzing exposure data with values below the detection limits are well described in the occupational hygiene literature, but an evaluation of a Bayesian approach for handling such data is currently lacking. Here, we first describe a Bayesian framework for analyzing censored data. We then present the results of a simulation study conducted to compare the β-substitution method with a Bayesian method for exposure datasets drawn from lognormal distributions and mixed lognormal distributions with varying sample sizes, geometric standard deviations (GSDs), and censoring for single and multiple limits of detection. For each set of factors, estimates for the arithmetic mean (AM), geometric mean, GSD, and the 95th percentile (X0.95) of the exposure distribution were obtained. We evaluated the performance of each method using relative bias, the root mean squared error (rMSE), and coverage (the proportion of the computed 95% uncertainty intervals containing the true value). The Bayesian method using non-informative priors and the β-substitution method were generally comparable in bias and rMSE when estimating the AM and GM. For the GSD and the 95th percentile, the Bayesian method with non-informative priors was more biased and had a higher rMSE than the β-substitution method, but use of more informative priors generally improved the Bayesian method’s performance, making both the bias and the rMSE more comparable to the β-substitution method. An advantage of the Bayesian method is that it provided estimates of uncertainty for these parameters of interest and good coverage, whereas the β-substitution method only provided estimates of uncertainty for the AM, and coverage was not as consistent. Selection of one or the other method depends on the needs of the practitioner, the availability of prior information, and the distribution characteristics of the measurement data. We suggest the use of Bayesian methods if the practitioner has the computational resources and prior information, as the method would generally provide accurate estimates and also provides the distributions of all of the parameters, which could be useful for making decisions in some applications. PMID:26209598

A comparative study of gallstones from children and adults using FTIR spectroscopy and fluorescence microscopy

PubMed Central

Kleiner, Oleg; Ramesh, Jagannathan; Huleihel, Mahmoud; Cohen, Beny; Kantarovich, Keren; Levi, Chen; Polyak, Boris; Marks, Robert S; Mordehai, Jacov; Cohen, Zahavi; Mordechai, Shaul

2002-01-01

Background Cholelithiasis is the gallstone disease (GSD) where stones are formed in the gallbladder. The main function of the gallbladder is to concentrate bile by the absorption of water and sodium. GSD has high prevalence among elderly adults. There are three major types of gallstones found in patients, White, Black and Brown. The major chemical component of white stones is cholesterol. Black and brown stones contain different proportions of cholesterol and bilirubin. The pathogenesis of gallstones is not clearly understood. Analysis of the chemical composition of gallstones using various spectroscopic techniques offers clues to the pathogenesis of gallstones. Recent years has seen an increasing trend in the number of cases involving children. The focus of this study is on the analysis of the chemical composition of gallstones from child and adult patients using spectroscopic methods. Methods In this report, we present FTIR spectroscopic studies and fluorescence microscopic analysis of gallstones obtained from 67 adult and 21 child patients. The gallstones were removed during surgical operations at Soroka University Medical Center. Results Our results show that black stones from adults and children are rich in bilirubin. Brown stones are composed of varying amounts of bilirubin and cholesterol. Green stones removed from an adult, which is rare, was found to be composed mainly of cholesterol. Our results also indicated that cholesterol and bilirubin could be the risk factors for gallstone formation in adults and children respectively. Fluorescence micrographs showed that the Ca-bilirubinate was present in all stones in different quantities and however, Cu-bilirubinate was present only in the mixed and black stones. Conclusions Analysis based on FTIR suggest that the composition of black and brown stones from both children and adults are similar. Various layers of the brown stone from adults differ by having varying quantities of cholesterol and calcium carbonate. Ring patterns observed mainly in the green stone using fluorescence microscopy have relevance to the mechanism of the stone formation. Our preliminary study suggests that bilirubin and cholesterol are the main risk factors of gallstone disease. PMID:11872150

GAMBLINGLESS: FOR LIFE study protocol: a pragmatic randomised trial of an online cognitive-behavioural programme for disordered gambling.

PubMed

Merkouris, S S; Rodda, S N; Austin, D; Lubman, D I; Harvey, P; Battersby, M; Cunningham, J; Lavis, T; Smith, D; Dowling, N A

2017-02-23

The prevalence of disordered gambling worldwide has been estimated at 2.3%. Only a small minority of disordered gamblers seek specialist face-to-face treatment, and so a need for alternative treatment delivery models that capitalise on advances in communication technology, and use self-directed activity that can complement existing services has been identified. As such, the primary aim of this study is to evaluate an online self-directed cognitive-behavioural programme for disordered gambling (GamblingLess: For Life). The study will be a 2-arm, parallel group, pragmatic randomised trial. Participants will be randomly allocated to a pure self-directed (PSD) or guided self-directed (GSD) intervention. Participants in both groups will be asked to work through the 4 modules of the GamblingLess programme over 8 weeks. Participants in the GSD intervention will also receive weekly emails of guidance and support from a gambling counsellor. A total of 200 participants will be recruited. Participants will be eligible if they reside in Australia, are aged 18 years and over, have access to the internet, have adequate knowledge of the English language, are seeking help for their own gambling problems and are willing to take part in the intervention and associated assessments. Assessments will be conducted at preintervention, and at 2, 3 and 12 months from preintervention. The primary outcome is gambling severity, assessed using the Gambling Symptom Assessment Scale. Secondary outcomes include gambling frequency, gambling expenditure, psychological distress, quality of life and additional help-seeking. Qualitative interviews will also be conducted with a subsample of participants and the Guides (counsellors). The study has been approved by the Deakin University Human Research and Eastern Health Human Research Ethics Committees. Findings will be disseminated via report, peer-reviewed publications and conference presentations. ACTRN12615000864527; results. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

The Physiopathological Role of the Exchangers Belonging to the SLC37 Family

NASA Astrophysics Data System (ADS)

Cappello, Anna Rita; Curcio, Rosita; Lappano, Rosamaria; Maggiolini, Marcello; Dolce, Vincenza

2018-04-01

The human SLC37 gene family includes four proteins SLC37A1-4, localized in the endoplasmic reticulum (ER) membrane. They have been grouped into the SLC37 family due to their sequence homology to the bacterial organophosphate/phosphate (Pi) antiporter. SLC37A1-3 are the less characterized isoforms. SLC37A1 and SLC37A2 are Pi-linked glucose-6-phosphate (G6P) antiporters, catalyzing both homologous (Pi/Pi) and heterologous (G6P/Pi) exchanges, whereas SLC37A3 transport properties remain to be clarified. Furthermore, SLC37A1 is highly homologous to the bacterial glycerol 3-phosphate permeases, so it is supposed to transport also glycerol-3-phosphate. The physiological role of SLC37A1-3 is yet to be further investigated. SLC37A1 seems to be required for lipid biosynthesis in cancer cell lines, SLC37A2 has been proposed as a vitamin D and a phospho-progesterone receptor target gene, while mutations in the SLC37A3 gene appear to be associated with congenital hyperinsulinism of infancy. SLC37A4, also known as glucose-6-phosphate translocase (G6PT), transports G6P from the cytoplasm into the ER lumen, working in complex with either glucose-6-phosphatase-α (G6Pase-α) or G6Pase-β to hydrolyze intraluminal G6P to Pi and glucose. G6PT and G6Pase-β are ubiquitously expressed, whereas G6Pase-α is specifically expressed in the liver, kidney and intestine. G6PT/G6Pase-α complex activity regulates fasting blood glucose levels, whereas G6PT/G6Pase-β is required for neutrophil functions. G6PT deficiency is responsible for glycogen storage disease type Ib (GSD-Ib), an autosomal recessive disorder associated with both defective metabolic and myeloid phenotypes. Several kinds of mutations have been identified in the SLC37A4 gene, affecting G6PT function. An increased autoimmunity risk for GSD-Ib patients has also been reported, moreover, SLC37A4 seems to be involved in autophagy.

Exploring the effects of temperature and grain size on plumes associated with PDCs through analogue experimentation

NASA Astrophysics Data System (ADS)

Mitchell, S. J.; Eychenne, J.; Rust, A.

2015-12-01

Pyroclastic density currents (PDCs) often loft upwards into convective, buoyant co-PDC plumes. Recent analogue experiments using a unimodal grain size of 22 ± 6 μm (Andrews & Manga, 2012) have established that plume generation is aided by PDC interaction with a topographic barrier. Here, we have simulated the onset of co-PDC plumes from the collapse of concentrated particle-gas mixtures comprised of unimodal or bimodal grain size distributions (GSD) of glass beads, using combinations of lognormal populations with modes of 35, 195 and 590 μm. The collapse of a mixture, with constant mass 2950 ± 150 g, induced the propagation of a gravity current channelized down a 13° sloping tank; a barrier in the tank caused the gravity current to produce a plume of particles. Experiments were recorded with high speed visible and thermal-infrared cameras. Initial GSD and temperature of the mixture were varied to assess the effects of the addition of a coarser component on plume generation. Analogue co-PDC plumes were only produced when a proportion of fine grains (35 μm) was present in the initial granular mixture. Sampling of the particles entrained in the co-PDC plumes revealed that fine grains (35 μm) are preferentially lofted, although a few coarser particles (195 or 590 μm) are also entrained in the co-PDC plumes and settle closer to the area of uplift. Increasing the initial temperature of the mixture increases plume height measured at 1 and 2s after onset; this is supported by repeat experiments at specific conditions. Bimodal mixtures containing both fine (35 μm) and coarser (195 or 590 μm) grains result in plume heights and initial flow velocities higher than observed in unimodal fine-grained experiments of the same total mass of particles. Repeat experiments identify the natural variability in plume generation under the same nominal conditions, which is likely due to the combined variations of momentum during flow propagation and heat-driven buoyancy, as well as the homogeneity of the initial particle mixture.

Associations between petrol-station density and manganese and lead in the cord blood of newborns living in Taiwan.

PubMed

Lin, Ying-Ying; Guo, Yue-Liang Leon; Chen, Pau-Chung; Liu, Jyung-Hung; Wu, Hui-Chen; Hwang, Yaw-Huei

2011-02-01

Although the anti-knocking agents used in Taiwan do not contain manganese, there are relatively high concentrations of the element in diesel fuel. As such, there have been many concerns about the impact of exposure to diesel fuels on health. This study was conducted in Taiwan to investigate the relationship between the concentration of manganese in cord blood of Taiwanese newborns and the geographic density of petrol stations as a surrogate for determining manganese emissions from vehicular traffic. A total of 1526 full-term newborns without major congenital malformations were consecutively recruited from various medical facilities from May 2004 to July 2005. Questionnaires were completed by the newborns' mothers after delivery to collect information on demographic characteristics, medical history, living environment, and other factors. Cord blood samples were collected at birth and analyzed for manganese and lead using inductively coupled plasma mass spectrometry. The geographic density of petrol stations within a 10 km zone around each newborn's residence was calculated for 1343 newborns using the Arc9 Geographic Information System. The geometric means of cord blood manganese and lead concentrations were 47.0 μg/L (GSD=1.42) and 12.6 μg/L (GSD=1.76), respectively. After adjusting for potential confounding factors, including maternal age, and maternal education, the results of a multiple linear regression model indicated that the concentration of cord blood manganese increased monotonically with an increasing density of petrol stations. However, no such association was found for levels of lead in cord blood. Further smoothing spline model analysis indicated that a ten unit increment in petrol station density made cord blood manganese and lead levels change by factors of 1.0092 (95% CI: 1.0058, 1.0127) and 0.9994 (95% CI: 0.9890, 0.9998), respectively. This finding suggests that exposure to manganese-containing fuel from motor vehicles may result in elevated manganese levels in the fetus. Further research is warranted to explore the relationship between traffic-related manganese exposure and potential adverse effects on fetal development. Copyright © 2011 Elsevier Inc. All rights reserved.

Accuracy Potential and Applications of MIDAS Aerial Oblique Camera System

NASA Astrophysics Data System (ADS)

Madani, M.

2012-07-01

Airborne oblique cameras such as Fairchild T-3A were initially used for military reconnaissance in 30s. A modern professional digital oblique camera such as MIDAS (Multi-camera Integrated Digital Acquisition System) is used to generate lifelike three dimensional to the users for visualizations, GIS applications, architectural modeling, city modeling, games, simulators, etc. Oblique imagery provide the best vantage for accessing and reviewing changes to the local government tax base, property valuation assessment, buying & selling of residential/commercial for better decisions in a more timely manner. Oblique imagery is also used for infrastructure monitoring making sure safe operations of transportation, utilities, and facilities. Sanborn Mapping Company acquired one MIDAS from TrackAir in 2011. This system consists of four tilted (45 degrees) cameras and one vertical camera connected to a dedicated data acquisition computer system. The 5 digital cameras are based on the Canon EOS 1DS Mark3 with Zeiss lenses. The CCD size is 5,616 by 3,744 (21 MPixels) with the pixel size of 6.4 microns. Multiple flights using different camera configurations (nadir/oblique (28 mm/50 mm) and (50 mm/50 mm)) were flown over downtown Colorado Springs, Colorado. Boresight fights for 28 mm nadir camera were flown at 600 m and 1,200 m and for 50 mm nadir camera at 750 m and 1500 m. Cameras were calibrated by using a 3D cage and multiple convergent images utilizing Australis model. In this paper, the MIDAS system is described, a number of real data sets collected during the aforementioned flights are presented together with their associated flight configurations, data processing workflow, system calibration and quality control workflows are highlighted and the achievable accuracy is presented in some detail. This study revealed that the expected accuracy of about 1 to 1.5 GSD (Ground Sample Distance) for planimetry and about 2 to 2.5 GSD for vertical can be achieved. Remaining systematic errors were modeled by analyzing residuals using correction grid. The results of the final bundle adjustments are sufficient to enable Sanborn to produce DEM/DTM and orthophotos from the nadir imagery and create 3D models using georeferenced oblique imagery.

High performance optical payloads for microsatellites

NASA Astrophysics Data System (ADS)

Geyl, Roland; Rodolfo, Jacques; Girault, Jean-Philippe

2017-09-01

Safran is presenting two concepts of optical payloads for microsatellites combining high performances and extremely compact volume. The first one offer 10-m Ground Sampling Distance (GSD) over 60x40 km2 area from 600 km orbit optimized for twilight conditions. The second one is offering a much higher resolution of 1.8-m over 11x7,5 km2 area from the same 600 km orbit. The two concepts are based on advanced innovative diffraction limited optical system packaged in a unique very compact volume lower than 8U = 200x200x200 mm making them the ideal solution for 15- 100 kg microsatellites. The maximum number of pixels is served to the end-user space imagery community thanks to 35 mm Full Frame sensors offering, as of today, 6000x4000 pixels. Up to 10 spectral bands from 475 to 900 nm can be offered thanks to 2D structured filters.

The Prisma Hyperspectra Mission

NASA Astrophysics Data System (ADS)

Loizzo, R.; Ananasso, C.; Guarini, R.; Lopinto, E.; Candela, L.; Pisani, A. R.

2016-08-01

PRISMA (PRecursore IperSpettrale della Missione Applicativa) is an Italian Space Agency (ASI) hyperspectral mission currently scheduled for the lunch in 2018. PRISMA is a single satellite placed on a sun- synchronous Low Earth Orbit (620 km altitude) with an expected operational lifetime of 5 years. The hyperspectral payload consists of a high spectral resolution (VNIR-SWIR) imaging spectrometer, optically integrated with a medium resolution Panchromatic camera. PRISMA will acquire data on areas of 30 km Swath width and with a Ground Sampling Distance (GSD) of 30 m (hyperspectral) and of 5 m Panchromatic (PAN). The PRISMA Ground Segment will be geographically distributed between Fucino station and ASI Matera Space Geodesy Centre and will include the Mission Control Centre, the Satellite Control Centre and the Instrument Data Handling System. The science community supports the overall lifecycle of the mission, being involved in algorithms definition, calibration and validation activities, research and applications development.

NAOMI instrument: a product line of compact and versatile cameras designed for HR and VHR missions in Earth observation

NASA Astrophysics Data System (ADS)

Luquet, Ph.; Brouard, L.; Chinal, E.

2017-11-01

Astrium has developed a product line of compact and versatile instruments for HR and VHR missions in Earth Observation. These cameras consist on a Silicon Carbide Korsch-type telescope, a focal plane with one or several retina modules - including five lines CCD, optical filters and front end electronics - and the instrument main electronics. Several versions have been developed with a telescope pupil diameter from 200 mm up to 650 mm, covering a large range of GSD (from 2.5 m down to sub-metric) and swath (from 10km up to 30 km) and compatible with different types of platform. Nine cameras have already been manufactured for five different programs: ALSAT2 (Algeria), SSOT (Chile), SPOT6 & SPOT7 (France), KRS (Kazakhstan) and VNREDSat (Vietnam). Two of them have already been launched and are delivering high quality images.

Did Lizards Follow Unique Pathways in Sex Chromosome Evolution?

PubMed Central

Gleeson, Dianne; Georges, Arthur

2018-01-01

Reptiles show remarkable diversity in modes of reproduction and sex determination, including high variation in the morphology of sex chromosomes, ranging from homomorphic to highly heteromorphic. Additionally, the co-existence of genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) within and among sister clades makes this group an attractive model to study and understand the evolution of sex chromosomes. This is particularly so with Lizards (Order Squamata) which, among reptiles, show extraordinary morphological diversity. They also show no particular pattern of sex chromosome degeneration of the kind observed in mammals, birds and or even in snakes. We therefore speculate that sex determination sensu sex chromosome evolution is labile and rapid and largely follows independent trajectories within lizards. Here, we review the current knowledge on the evolution of sex chromosomes in lizards and discuss how sex chromosome evolution within that group differs from other amniote taxa, facilitating unique evolutionary pathways. PMID:29751579

Where size does matter: foldable telescope design for microsat application

NASA Astrophysics Data System (ADS)

Segert, Tom; Danziger, Björn; Lieder, Matthias

2017-11-01

The DOBSON SPACE TELESCOPE Project (DST) at the Technical University of Berlin (TUB) believes that micro satellites can be a challenging competitor in the high resolution remote sensing market. Using a micro satellite as basis for a remote sensing platform will dramatically reduce the cost for the end users thereby initiating the predicted remote sensing boom. The Challenging task is that an optic required for a GSD smaller than 1m is much bigger than the given room for secondary payload. In order to break the volume limits of hitchhiker payloads the DST team develops an optical telescope with deployable structures. The core piece of DST is a 20 inch modified Cassegrain optic. Stored during ascend the instrument fits in a box measuring 60 x 60 x 30cm (including telescope and optical plane assembly). After the satellite was released into free space the telescope unfolds and collimates automatically.

Band-Moment Compression of AVIRIS Hyperspectral Data and its Use in the Detection of Vegetation Stress

NASA Technical Reports Server (NTRS)

Estep, L.; Davis, B.

2001-01-01

A remote sensing campaign was conducted over a U.S. Department of Agriculture test farm at Shelton, Nebraska. An experimental field was set off in plots that were differentially treated with anhydrous ammonia. Four replicates of 0-kg/ha to 200-kg/ha plots, in 50-kg/ha increments, were set out in a random block design. Low-altitude (GSD of 3 m) Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) hyperspectral data were collected over the site in 224 bands. Simultaneously, ground data were collected to support the airborne imagery. In an effort to reduce data load while maintaining or enhancing algorithm performance for vegetation stress detection, band-moment compression and analysis was applied to the AVIRIS image cube. The results indicated that band-moment techniques compress the AVIRIS dataset significantly while retaining the capability of detecting environmentally induced vegetation stress.

Impact of random discrete dopant in extension induced fluctuation in gate-source/drain underlap FinFET

NASA Astrophysics Data System (ADS)

Wang, Yijiao; Huang, Peng; Xin, Zheng; Zeng, Lang; Liu, Xiaoyan; Du, Gang; Kang, Jinfeng

2014-01-01

In this work, three dimensional technology computer-aided design (TCAD) simulations are performed to investigate the impact of random discrete dopant (RDD) including extension induced fluctuation in 14 nm silicon-on-insulator (SOI) gate-source/drain (G-S/D) underlap fin field effect transistor (FinFET). To fully understand the RDD impact in extension, RDD effect is evaluated in channel and extension separately and together. The statistical variability of FinFET performance parameters including threshold voltage (Vth), subthreshold slope (SS), drain induced barrier lowering (DIBL), drive current (Ion), and leakage current (Ioff) are analyzed. The results indicate that RDD in extension can lead to substantial variability, especially for SS, DIBL, and Ion and should be taken into account together with that in channel to get an accurate estimation on RDF. Meanwhile, higher doping concentration of extension region is suggested from the perspective of overall variability control.

Novel approaches to develop Rift Valley fever vaccines

PubMed Central

Indran, Sabarish V.; Ikegami, Tetsuro

2012-01-01

Rift Valley fever (RVF) is endemic to sub-Saharan Africa, and has spread into Madagascar, Egypt, Saudi Arabia, and Yemen. Rift Valley fever virus (RVFV) of the family Bunyaviridae, genus Phlebovirus causes hemorrhagic fever, neurological disorders or blindness in humans, and high rate abortion and fetal malformation in ruminants. RVFV is classified as a Category A Priority pathogen and overlap select agent by CDC/USDA due to its potential impact on public health and agriculture. There is a gap in the safety and immunogenicity in traditional RVF vaccines; the formalin-inactivated RVFV vaccine TSI-GSD-200 requires three doses for protection, and the live-attenuated Smithburn vaccine has a risk to cause abortion and fetal malformation in pregnant ruminants. In this review, problems of traditional vaccines and the safety and efficacy of recently reported novel RVF candidate vaccines including subunit vaccines, virus vector, and replicons are discussed. PMID:23112960

Reconstitution radicicol containing apolipoprotein B lipoparticle and tracing its cell uptake process by super resolution fluorescent microscopy.

NASA Astrophysics Data System (ADS)

Lin, Chung Ching; Lin, Po-Yen; Chang, Chia-Ching

Apolipoprotein B (apoB) is the only protein of LDL. LDL delivers cholesterol, triacylglycerides and lipids to the target cells. Reconstitute apoB lipoparticle (rABL) will be an idea drug delivery vehicle for hydrophobic and amphiphilic materials delivery. It is challenged to renature ApoB into its functional state from denatured state. By using modified bile salt and radicicol (Rad) added over-critical refolding process, apoB can be restored into its native like state. The intrinsic fluorescence of apoB increased during the refolding process. Moreover, radicicol (Rad) molecules have been encapsulated into reconstitute rABL (Rad@rABL). To investigate the cell uptake mechanism of Rad@rABL, a super resolution ground state depletion (GSD) microscopy is used in this research. Fluorescence labeled Rad@rABL can be traced within the tumor cell. Key words: LDL, radicicol, protein refolding, super resolution microscopy.

Lack of Glycogenin Causes Glycogen Accumulation and Muscle Function Impairment.

PubMed

Testoni, Giorgia; Duran, Jordi; García-Rocha, Mar; Vilaplana, Francisco; Serrano, Antonio L; Sebastián, David; López-Soldado, Iliana; Sullivan, Mitchell A; Slebe, Felipe; Vilaseca, Marta; Muñoz-Cánoves, Pura; Guinovart, Joan J

2017-07-05

Glycogenin is considered essential for glycogen synthesis, as it acts as a primer for the initiation of the polysaccharide chain. Against expectations, glycogenin-deficient mice (Gyg KO) accumulate high amounts of glycogen in striated muscle. Furthermore, this glycogen contains no covalently bound protein, thereby demonstrating that a protein primer is not strictly necessary for the synthesis of the polysaccharide in vivo. Strikingly, in spite of the higher glycogen content, Gyg KO mice showed lower resting energy expenditure and less resistance than control animals when subjected to endurance exercise. These observations can be attributed to a switch of oxidative myofibers toward glycolytic metabolism. Mice overexpressing glycogen synthase in the muscle showed similar alterations, thus indicating that this switch is caused by the excess of glycogen. These results may explain the muscular defects of GSD XV patients, who lack glycogenin-1 and show high glycogen accumulation in muscle. Copyright © 2017 Elsevier Inc. All rights reserved.

Novel approaches to develop Rift Valley fever vaccines.

PubMed

Indran, Sabarish V; Ikegami, Tetsuro

2012-01-01

Rift Valley fever (RVF) is endemic to sub-Saharan Africa, and has spread into Madagascar, Egypt, Saudi Arabia, and Yemen. Rift Valley fever virus (RVFV) of the family Bunyaviridae, genus Phlebovirus causes hemorrhagic fever, neurological disorders or blindness in humans, and high rate abortion and fetal malformation in ruminants. RVFV is classified as a Category A Priority pathogen and overlap select agent by CDC/USDA due to its potential impact on public health and agriculture. There is a gap in the safety and immunogenicity in traditional RVF vaccines; the formalin-inactivated RVFV vaccine TSI-GSD-200 requires three doses for protection, and the live-attenuated Smithburn vaccine has a risk to cause abortion and fetal malformation in pregnant ruminants. In this review, problems of traditional vaccines and the safety and efficacy of recently reported novel RVF candidate vaccines including subunit vaccines, virus vector, and replicons are discussed.

BILSAT-1: A low-cost, agile, earth observation microsatellite for Turkey

NASA Astrophysics Data System (ADS)

Bradford, Andy; Gomes, Luis M.; Sweeting, Martin; Yuksel, Gokhan; Ozkaptan, Cem; Orlu, Unsal

2003-08-01

TUBITAK-BIlTEN has initaited a project to develop and propagate small satallite technologies in Turkey.As part of this initiative, TUBITAK-BILTEN is working working SSTL to develop a 100kg class enhanced microsatelliote, BILSAT-1. With the successful completion of this project, TUBITAK-BILTEN will be capable of producing its own satellites, covering all phases from design from design to production and in-orbit operatiion. It is hoped that acquisition of these technologies will stimulate Turkish industry into greater involvement in space related activities. The project was started in August 2001 and will run through to February 2003 with launch scheduled for July 2003. BILSAT-1 will be one of the most capable satellites that SSTL have eveer built and features several technologies normally only found on larger satellites. Specifically, the Attitude Determination and Control System of BILSAT-1 will be the most advanced that SSTL have everflown: Dual redundant Star Cameras, sun sensors and rate gyros provide accurate and precise attitude information allowing a very high degree of attitude knowledge. Acruators on board will make the satellite extremely agile — for instance allowing fast slew manoeuvers about its roll and pitch axes. The agile control system also enables ground target revisit times to be reduced compared to nadir-pointing gravity gradient stabilized satellites, and will allow stereoscopic imaging, target tracking and multiple attitude imaging to be undertaken with the satellites prime payloads: a 4-band multispectral 26-metre GSD imaging system and a 12-metre GSD panchromatic imager. Also on board the satellite there are additional payloads, including a state-of-the-art Digital Signal Processing payload (GEZGIN) that will enabele real time image compression in JPEG2000 format using a high performance floating point DSP, and a low resolution multi spectral (9-band) camera (COBAN). BILSAT-1 will also co-operate in the international Disaster Monitoring Constellation (DMC) led by SSTL, providing the ability to enhance the imaging capabilities of the constellation. In parallel with the microsatellite design and build activities, all the infrastructure required to design, produce and operate a satellite is being constructed at BILTEN's premises in Turkey. This infrastructure includes assembly and integration rooms, a PCB prototyping workshop, research and development laboratories, and a satellite mission control ground station.

Diesel exhaust exposures in port workers.

PubMed

Debia, Maximilien; Neesham-Grenon, Eve; Mudaheranwa, Oliver C; Ragettli, Martina S

2016-07-01

Exposure to diesel engine exhaust has been linked to increased cancer risk and cardiopulmonary diseases. Diesel exhaust is a complex mixture of chemical substances, including a particulate fraction mainly composed of ultrafine particles, resulting from the incomplete combustion of fuel. Diesel trucks are known to be an important source of diesel-related air pollution, and areas with heavy truck traffic are associated with higher air pollution levels and increased public health problems. Several indicators have been proposed as surrogates for estimating exposures to diesel exhaust but very few studies have focused specifically on monitoring the ultrafine fraction through the measurement of particle number concentrations. The aim of this study is to assess occupational exposures of gate controllers at the port of Montreal, Canada, to diesel engine emissions from container trucks by measuring several surrogates through a multimetric approach which includes the assessment of both mass and number concentrations and the use of direct reading devices. A 10-day measurement campaign was carried out at two terminal checkpoints at the port of Montreal. Respirable elemental and organic carbon, PM1, PM2.5, PMresp (PM4), PM10, PMtot (inhalable fraction), particle number concentrations, particle size distributions, and gas concentrations (NO2, NO, CO) were monitored. Gate controllers were exposed to concentrations of contaminants associated with diesel engine exhaust (elemental carbon GM = 1.6 µg/m(3); GSD = 1.6) well below recommended occupational exposure limits. Average daily particle number concentrations ranged from 16,544-67,314 particles/cm³ (GM = 32,710 particles/cm³; GSD = 1.6). Significant Pearson correlation coefficients were found between daily elemental carbon, PM fractions and particle number concentrations, as well as between total carbon, PM fractions and particle number concentrations. Significant correlation coefficients were found between particle number concentrations and the number of trucks and wind speed (R(2) = 0.432; p < 0.01). The presence of trucks with cooling systems and older trucks with older exhaust systems was associated with peak concentrations on the direct reading instruments. The results highlight the relevance of direct reading instruments in helping to identify sources of exposure and suggest that monitoring particle number concentrations improves understanding of workers' exposures to diesel exhaust. This study, by quantifying workers' exposure levels through a multimetric approach, contributes to the further understanding of occupational exposures to diesel engine exhaust.

A Comparison of the β-Substitution Method and a Bayesian Method for Analyzing Left-Censored Data.

PubMed

Huynh, Tran; Quick, Harrison; Ramachandran, Gurumurthy; Banerjee, Sudipto; Stenzel, Mark; Sandler, Dale P; Engel, Lawrence S; Kwok, Richard K; Blair, Aaron; Stewart, Patricia A

2016-01-01

Classical statistical methods for analyzing exposure data with values below the detection limits are well described in the occupational hygiene literature, but an evaluation of a Bayesian approach for handling such data is currently lacking. Here, we first describe a Bayesian framework for analyzing censored data. We then present the results of a simulation study conducted to compare the β-substitution method with a Bayesian method for exposure datasets drawn from lognormal distributions and mixed lognormal distributions with varying sample sizes, geometric standard deviations (GSDs), and censoring for single and multiple limits of detection. For each set of factors, estimates for the arithmetic mean (AM), geometric mean, GSD, and the 95th percentile (X0.95) of the exposure distribution were obtained. We evaluated the performance of each method using relative bias, the root mean squared error (rMSE), and coverage (the proportion of the computed 95% uncertainty intervals containing the true value). The Bayesian method using non-informative priors and the β-substitution method were generally comparable in bias and rMSE when estimating the AM and GM. For the GSD and the 95th percentile, the Bayesian method with non-informative priors was more biased and had a higher rMSE than the β-substitution method, but use of more informative priors generally improved the Bayesian method's performance, making both the bias and the rMSE more comparable to the β-substitution method. An advantage of the Bayesian method is that it provided estimates of uncertainty for these parameters of interest and good coverage, whereas the β-substitution method only provided estimates of uncertainty for the AM, and coverage was not as consistent. Selection of one or the other method depends on the needs of the practitioner, the availability of prior information, and the distribution characteristics of the measurement data. We suggest the use of Bayesian methods if the practitioner has the computational resources and prior information, as the method would generally provide accurate estimates and also provides the distributions of all of the parameters, which could be useful for making decisions in some applications. © The Author 2015. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

PLÉIADES Project: Assessment of Georeferencing Accuracy, Image Quality, Pansharpening Performence and Dsm/dtm Quality

NASA Astrophysics Data System (ADS)

Topan, Hüseyin; Cam, Ali; Özendi, Mustafa; Oruç, Murat; Jacobsen, Karsten; Taşkanat, Talha

2016-06-01

Pléiades 1A and 1B are twin optical satellites of Optical and Radar Federated Earth Observation (ORFEO) program jointly running by France and Italy. They are the first satellites of Europe with sub-meter resolution. Airbus DS (formerly Astrium Geo) runs a MyGIC (formerly Pléiades Users Group) program to validate Pléiades images worldwide for various application purposes. The authors conduct three projects, one is within this program, the second is supported by BEU Scientific Research Project Program, and the third is supported by TÜBİTAK. Assessment of georeferencing accuracy, image quality, pansharpening performance and Digital Surface Model/Digital Terrain Model (DSM/DTM) quality subjects are investigated in these projects. For these purposes, triplet panchromatic (50 cm Ground Sampling Distance (GSD)) and VNIR (2 m GSD) Pléiades 1A images were investigated over Zonguldak test site (Turkey) which is urbanised, mountainous and covered by dense forest. The georeferencing accuracy was estimated with a standard deviation in X and Y (SX, SY) in the range of 0.45m by bias corrected Rational Polynomial Coefficient (RPC) orientation, using ~170 Ground Control Points (GCPs). 3D standard deviation of ±0.44m in X, ±0.51m in Y, and ±1.82m in Z directions have been reached in spite of the very narrow angle of convergence by bias corrected RPC orientation. The image quality was also investigated with respect to effective resolution, Signal to Noise Ratio (SNR) and blur coefficient. The effective resolution was estimated with factor slightly below 1.0, meaning that the image quality corresponds to the nominal resolution of 50cm. The blur coefficients were achieved between 0.39-0.46 for triplet panchromatic images, indicating a satisfying image quality. SNR is in the range of other comparable space borne images which may be caused by de-noising of Pléiades images. The pansharpened images were generated by various methods, and are validated by most common statistical metrics and also visual interpretation. The generated DSM and DTM were achieved with ±1.6m standard deviation in Z (SZ) in relation to a reference DTM.

Manganese Fractionation Using a Sequential Extraction Method to Evaluate Welders' Shielded Metal Arc Welding Exposures During Construction Projects in Oil Refineries.

PubMed

Hanley, Kevin W; Andrews, Ronnee; Bertke, Steven; Ashley, Kevin

2015-01-01

The National Institute for Occupational Safety and Health has conducted an occupational exposure assessment study of manganese (Mn) in welding fume of construction workers rebuilding tanks, piping, and process equipment at two oil refineries. The objective of this study was to evaluate exposures to different Mn fractions using a sequential extraction procedure. Seventy-two worker-days were monitored for either total or respirable Mn during stick welding and associated activities both within and outside of confined spaces. The samples were analyzed using an experimental method to separate different Mn fractions by valence states based on selective chemical solubility. The full-shift total particulate Mn time-weighted average (TWA) breathing zone concentrations ranged from 0.013-29 for soluble Mn in a mild ammonium acetate solution; from 0.26-250 for Mn(0,2+) in acetic acid; from non-detectable (ND) - 350 for Mn(3+,4+) in hydroxylamine-hydrochloride; and from ND - 39 micrograms per cubic meter (μg/m(3)) for insoluble Mn fractions in hydrochloric and nitric acid. The summation of all Mn fractions in total particulate TWA ranged from 0.52-470 μg/m(3). The range of respirable particulate Mn TWA concentrations were from 0.20-28 for soluble Mn; from 1.4-270 for Mn(0,2+); from 0.49-150 for Mn(3+,4+); from ND - 100 for insoluble Mn; and from 2.0-490 μg/m(3) for Mn (sum of fractions). For all jobs combined, total particulate TWA GM concentrations of the Mn(sum) were 99 (GSD = 3.35) and 8.7 (GSD = 3.54) μg/m(3) for workers inside and outside of confined spaces; respirable Mn also showed much higher levels for welders within confined spaces. Regardless of particle size and confined space work status, Mn(0,2+) fraction was the most abundant followed by Mn(3+,4+) fraction, typically >50% and ∼30-40% of Mn(sum), respectively. Eighteen welders' exposures exceeded the ACGIH Threshold Limit Values for total Mn (100 μg/m(3)) and 25 exceeded the recently adopted respirable Mn TLV (20 μg/m(3)). This study shows that a welding fume exposure control and management program is warranted, especially for welding jobs in confined spaces.

Manganese fractionation using a sequential extraction method to evaluate welders’ shielded metal arc welding exposures during construction projects in oil refineries

PubMed Central

Hanley, Kevin W.; Andrews, Ronnee; Bertke, Steven; Ashley, Kevin

2015-01-01

The National Institute for Occupational Safety and Health (NIOSH) has conducted an occupational exposure assessment study of manganese (Mn) in welding fume of construction workers rebuilding tanks, piping, and process equipment at two oil refineries. The objective of this study was to evaluate exposures to different Mn fractions using a sequential extraction procedure. Seventy-two worker-days were monitored for either total or respirable Mn during stick welding and associated activities both within and outside of confined spaces. The samples were analyzed using an experimental method to separate different Mn fractions by valence states based on selective chemical solubility. The full-shift total particulate Mn time-weighted average (TWA) breathing zone concentrations ranged from 0.013 – 29 for soluble Mn in a mild ammonium acetate solution; from 0.26 – 250 for Mn0,2+ in acetic acid; from non-detectable (ND) – 350 for Mn3+,4+ in hydroxylamine-hydrochloride; and from ND – 39 micrograms per cubic meter (μg/m3) for insoluble Mn fractions in hydrochloric and nitric acid. The summation of all Mn fractions in total particulate TWA ranged from 0.52 to 470 μg/m3. The range of respirable particulate Mn TWA concentrations were from 0.20 – 28 for soluble Mn; from 1.4 – 270 for Mn0,2+; from 0.49 – 150 for Mn3+,4+; from ND – 100 for insoluble Mn; and from 2.0 – 490 μg/m3 for Mn (sum of fractions). For all jobs combined, total particulate TWA GM concentrations of the Mn(sum) were 99 (GSD=3.35) and 8.7 (GSD=3.54) μg/m3 for workers inside and outside of confined spaces; respirable Mn also showed much higher levels for welders within confined spaces. Regardless of particle size and confined space work status, Mn0,2+ fraction was the most abundant followed by Mn3+,4+ fraction, typically >50% and ~30-40% of Mn(sum), respectively. Eighteen welders’ exposures exceeded the ACGIH Threshold Limit Values for total Mn (100 μg/m3) and 25 exceeded the recently adopted respirable Mn TLV (20 μg/m3). This study shows that a welding fume exposure control and management program is warranted, especially for welding jobs in confined spaces. PMID:26011602

A coupled high-resolution modeling system to simulate biomass burning emissions, plume rise and smoke transport in real time over the contiguous US

NASA Astrophysics Data System (ADS)

Ahmadov, R.; Grell, G. A.; James, E.; Freitas, S.; Pereira, G.; Csiszar, I. A.; Tsidulko, M.; Pierce, R. B.; McKeen, S. A.; Saide, P.; Alexander, C.; Benjamin, S.; Peckham, S.

2016-12-01

Wildfires can have huge impact on air quality and visibility over large parts of the US. It is quite challenging to accurately predict wildfire air quality given significant uncertainties in modeling of biomass burning (BB) emissions, fire size, plume rise and smoke transport. We developed a new smoke modeling system (HRRR-Smoke) based on the coupled meteorology-chemistry model WRF-Chem. The HRRR-Smoke modeling system uses fire radiative power (FRP) data measured by the Visible Infrared Imaging Radiometer Suite (VIIRS) sensor on the Suomi National Polar-orbiting Partnership satellite. Using the FRP data enables predicting fire emissions, fire size and plume rise more accurately. Another advantage of the VIIRS data is the fire detection and characterization at­ high spatial resolution during both day and nighttime. The HRRR-Smoke model is run in real-time for summer 2016 on 3km horizontal grid resolution over CONUS domain by NOAA/ESRL Global Systems Division (GSD). The model simulates advection and mixing of fine particulate matter (PM2.5 or smoke) emitted by calculated BB emissions. The BB emissions include both smoldering and flaming fractions. Fire plume rise is parameterized in an online mode during the model integration. In addition to smoke, anthropogenic emissions of PM2.5 are transported in an inline mode as a passive tracer by HRRR-Smoke. The HRRR-Smoke real-time runs use meteorological fields for initial and lateral boundary conditions from the experimental real-time HRRR(X) numerical weather prediction model also run at NOAA/ESRL/GSD. The model is initialized every 6 hours (00, 06, 12 and 18UTC) daily using newly generated meteorological fields and FRP data obtained during previous 24 hours. Then the model produces meteorological and smoke forecasts for next 36 hours. The smoke fields are cycled from one forecast to the next one. Predicted near-surface and vertically integrated smoke concentrations are visualized online on a web-site: http://rapidrefresh.noaa.gov/HRRRsmoke/In this talk, we discuss the major components of the HRRR-Smoke modeling system. We present modeled smoke fields for some major wildfire cases over the western US in 2016 and discuss the model performance for those cases.

BILSAT-1: a Low-Cost Agile Earth Observation Microsatellite for Turkey

NASA Astrophysics Data System (ADS)

Bradford, Andy; Gomes, Luis M.; Sweeting, Martin, , Sir

TUBITAK-BILTEN has initiated a project to develop and propagate small satellite technologies in Turkey. As part of this initiative, TUBITAK-BILTEN is working with SSTL (UK) to develop a 100kg class microsatellite, BILSAT-1. With the successful completion of this project, TUBITAK-BILTEN will be capable of producing its own satellites, covering all phases from design to production. It is hoped that acquisition of these technologies will stimulate Turkish industry into greater involvement in space related activities. The project was started in August 2001 and will run through to launch scheduled for February 2003. BILSAT-1 will be one of the most capable microsatellites built by SSTL and features several technologies normally only found on larger satellites. Specifically, the Attitude Determination and Control System of BILSAT-1 will include dual-redundant star cameras, sun sensors and rate gyros to provide precise attitude information allowing very accurate attitude knowledge. Actuators on board will make the satellite extremely agile, allowing fast slew manoeuvres about its roll and pitch axes enabling the satellite to reduce imaging revisit times compared to fixed nadir-pointing gravity gradient stabilized satellites, and will allow novel and complex operations scenarios to be undertaken with the satellites prime payloads; a 26-metre GSD 4-band multispectral and a 12-metre GSD panchromatic imaging system. Stereoscopic imaging, target tracking and multiple attitude imaging are all operational scenarios that feature in the mission plan. Also on board the satellite are additional payloads, including a state-of-the-art Digital Signal Processing Board payload that will enable real time image compression in JPEG2000 format using a high performance floating point DSP, and a low resolution 9-band multispectral camera. BILSAT-1 will join the other 5 microsatellites in the SSTL-led international Disaster Monitoring Constellation (DMC), providing the ability to enhance the imaging capabilities of the constellation whose objective is to provide EO with daily revisit worldwide. In parallel with the Satellite design and build activities at the Surrey Space Centre &SSTL in the UK, all the infrastructure required to design, produce and operate a satellite, is being constructed at BILTEN's premises in Turkey. This infrastructure includes assembly and integration rooms, a PCB prototyping workshop, research and development laboratories, and a satellite mission control ground station.

Some safe and sensible shortcuts for efficiently upscaled updates of existing elevation models.

NASA Astrophysics Data System (ADS)

Knudsen, Thomas; Aasbjerg Nielsen, Allan

2013-04-01

The Danish national elevation model, DK-DEM, was introduced in 2009 and is based on LiDAR data collected in the time frame 2005-2007. Hence, DK-DEM is aging, and it is time to consider how to integrate new data with the current model in a way that improves the representation of new landscape features, while still preserving the overall (very high) quality of the model. In LiDAR terms, 2005 is equivalent to some time between the palaeolithic and the neolithic. So evidently, when (and if) an update project is launched, we may expect some notable improvements due to the technical and scientific developments from the last half decade. To estimate the magnitude of these potential improvements, and to devise efficient and effective ways of integrating the new and old data, we currently carry out a number of case studies based on comparisons between the current terrain model (with a ground sample distance, GSD, of 1.6 m), and a number of new high resolution point clouds (10-70 points/m2). Not knowing anything about the terms of a potential update project, we consider multiple scenarios ranging from business as usual: A new model with the same GSD, but improved precision, to aggressive upscaling: A new model with 4 times better GSD, i.e. a 16-fold increase in the amount of data. Especially in the latter case speeding up the gridding process is important. Luckily recent results from one of our case studies reveal that for very high resolution data in smooth terrain (which is the common case in Denmark), using local mean (LM) as grid value estimator is only negligibly worse than using the theoretically "best" estimator, i.e. ordinary kriging (OK) with rigorous modelling of the semivariogram. The bias in a leave one out cross validation differs on the micrometer level, while the RMSE differs on the 0.1 mm level. This is fortunate, since a LM estimator can be implemented in plain stream mode, letting the points from the unstructured point cloud (i.e. no TIN generation) stream through the processor, individually contributing to the nearest grid posts in a memory mapped grid file. Algorithmically this is very efficient, but it would be even more efficient if we did not have to handle so much data. Another of our recent case studies focuses on this. The basic idea is to ignore data that does not tell us anything new. We do this by looking at anomalies between the current height model and the new point cloud, then computing a correction grid for the current model. Points with insignificant anomalies are simply removed from the point cloud, and the correction grid is computed using the remaining point anomalies only. Hence, we only compute updates in areas of significant change, speeding up the process, and giving us new insight of the precision of the current model which in turn results in improved metadata for both the current and the new model. Currently we focus on simple approaches for creating a smooth update process for integration of heterogeneous data sets. On the other hand, as years go by and multiple generations of data become available, more advanced approaches will probably become necessary (e.g. a multi campaign bundle adjustment, improving the oldest data using cross-over adjustment with newer campaigns). But to prepare for such approaches, it is important already now to organize and evaluate the ancillary (GPS, INS) and engineering level data for the current data sets. This is essential if future generations of DEM users should be able to benefit from future conceptions of "some safe and sensible shortcuts for efficiently upscaled updates of existing elevation models".

DSM Generation from ALSO/PRISM Images Using SAT-PP

NASA Astrophysics Data System (ADS)

Wolff, Kirsten; Gruen, Armin

2008-11-01

One of the most important products of ALOS/PRISM image data are accurate DSMs. To exploit the full potential of the full resolution of PRISM for DSM generation, a highly developed image matcher is needed. As a member of the validation and calibration team for PRISM we published earlier results of DSM generation using PRISM image triplets in combination with our software package SAT-PP. The overall accuracy across all object and image features for all tests lies between 1-5 pixels in matching, depending primarily on surface roughness, vegetation, image texture and image quality. Here we will discuss some new results. We focus on four different topics: the use of two different evaluation methods, the difference between a 5m and a 10m GSD for the final PRISM DSM, the influence of the level of initial information and the comparison of the quality of different combinations of the three different views forward, nadir and backward. All tests have been conducted with our testfield Bern/Thun, Switzerland.

The Design and Evaluation of the Lighting Imaging Sensor Data Applications Display (LISDAD)

NASA Technical Reports Server (NTRS)

Boldi, B.; Hodanish, S.; Sharp, D.; Williams, E.; Goodman, Steven; Raghavan, R.; Matlin, A.; Weber, M.

1998-01-01

The design and evaluation of the Lightning Imaging Sensor Data Applications Display (LISDAD). The ultimate goal of the LISDAD system is to quantify the utility of total lightning information in short-term, severe-weather forecasting operations. To this end, scientists from NASA, NWS, and MIT organized an effort to study the relationship of lightning and severe-weather on a storm-by-storm, and even cell-by-cell basis for as many storms as possible near Melbourne, Florida. Melbourne was chosen as it offers a unique combination of high probability of severe weather and proximity to major relevant sensors - specifically: NASA's total lightning mapping system at Kennedy Space Center (the LDAR system at KSC); a NWS/NEXRAD radar (at Melbourne); and a prototype Integrated Terminal Weather System (ITWS, at Orlando), which obtains cloud-to-ground lightning Information from the National Lightning Detection Network (NLDN), and also uses NSSL's Severe Storm Algorithm (NSSL/SSAP) to obtain information about various storm-cell parameters. To assist in realizing this project's goal, an interactive, real-time data processing system (the LISDAD system) has been developed that supports both operational short-term weather forecasting and post facto severe-storm research. Suggestions have been drawn from the operational users (NWS/Melbourne) in the design of the data display and its salient behavior. The initial concept for the users Graphical Situation Display (GSD) was simply to overlay radar data with lightning data, but as the association between rapid upward trends in the total lightning rate and severe weather became evident, the display was significantly redesigned. The focus changed to support the display of time series of storm-parameter data and the automatic recognition of cells that display rapid changes in the total-lightning flash rate. The latter is calculated by grouping discrete LDAR radiation sources into lightning flashes using a time-space association algorithm. Specifically, the GSD presents the user with the Composite Maximum Reflectivity obtained from the NWS/NEXRAD. Superimposed upon this background image are placed small black circles indicating the locations of storm cells identified by the NSSL/SSA. The circles become cyan if lightning is detected within the storm-cell; if the cell has lightning rates indicative of a severe-storm, the circle turns red. This paper will: (1) review the design of LISDAD system; (2) present some examples of its data display; and shown results of the lightning based severe-weather prediction algorithm.

Human exposure to polyhexamethylene guanidine phosphate from humidifiers in residential settings: Cause of serious lung disease.

PubMed

Lee, Ji Hyun; Yu, Il Je

2017-11-01

Exposure to the humidifier disinfectant, polyhexamethylene guanidine phosphate (PHMG), in mists generated from ultrasonic humidifiers was studied in a simulation chamber and apartment rooms. PHMG is suspected as a causative agent of lung disease in Korea residences. In the simulation-chamber study, the amount of disinfectant discharged from three different ultrasonic humidifiers was measured. Mists generated at 1, 2, and 4 times the recommended amount of disinfectant were sampled with an impinger, and the effect of relative humidity (RH) on airborne disinfectant concentration was studied by changing RH from 60%-70% to 90%-100%. In addition, particle size distribution (PSD) in mists was measured by scanning mobility particle sizer (SMPS), aerodynamic particle sizer (APS), and Mastersizer. In the apartment study, mists generated from ultrasonic humidifiers were sampled for 6 h in small and large rooms during fall ( n = 10) and winter ( n = 15). In the simulation study, the humidifiers discharged 205 ± 24.6 ml/h of mist at maximum capacity. Concentrations of airborne disinfectant increased with increasing concentration of disinfectant. RH affected airborne disinfectant concentration in the chamber, with increasing concentration with increasing RH. Below RH 70%, no airborne PHMG was detected. PHMG-containing mists generated from ultrasonic humidifier showed various sizes ranging from 149-157 nm to 690-740 nm to larger than 5.4 µm by SMPS, APS, and Mastersizer, respectively. Surface area mean diameter measured by Mastersizer ranged from 5.39 µm to 5.72 µm. In the apartment study conducted during the fall, the geometric mean (GM) and geometric standard deviation (GSD) and arithmetic mean (AM) and standard deviation (SD) of airborne PHMG concentration were 3.22 + 5.13 µg/m 3 and 8.26 ± 12.18 µg/m 3 , respectively. In the winter, GM + GSD and AM ± SD of airborne PHMG concentration were 0.21 + 2.11 µg/m 3 and 0.35 ± 0.62 µg/m 3 , respectively. RH and temperature in the apartment rooms for fall and winter were 22.5 ± 1.7°C, 74.5 ± 15.6% and 22.0 ± 2°C, 51.1 ± 12.9%, respectively. Different RHs in the fall and winter resulted in very different airborne concentrations of disinfectant in the apartment rooms. Exposure levels and PSD of mists generated from ultrasonic humidifiers in apartments are not sufficient to conclude that PHMG causes lung disease in Korean residences.

Generic Sensor Modeling Using Pulse Method

NASA Technical Reports Server (NTRS)

Helder, Dennis L.; Choi, Taeyoung

2005-01-01

Recent development of high spatial resolution satellites such as IKONOS, Quickbird and Orbview enable observation of the Earth's surface with sub-meter resolution. Compared to the 30 meter resolution of Landsat 5 TM, the amount of information in the output image was dramatically increased. In this era of high spatial resolution, the estimation of spatial quality of images is gaining attention. Historically, the Modulation Transfer Function (MTF) concept has been used to estimate an imaging system's spatial quality. Sometimes classified by target shapes, various methods were developed in laboratory environment utilizing sinusoidal inputs, periodic bar patterns and narrow slits. On-orbit sensor MTF estimation was performed on 30-meter GSD Landsat4 Thematic Mapper (TM) data from the bridge pulse target as a pulse input . Because of a high resolution sensor s small Ground Sampling Distance (GSD), reasonably sized man-made edge, pulse, and impulse targets can be deployed on a uniform grassy area with accurate control of ground targets using tarps and convex mirrors. All the previous work cited calculated MTF without testing the MTF estimator's performance. In previous report, a numerical generic sensor model had been developed to simulate and improve the performance of on-orbit MTF estimating techniques. Results from the previous sensor modeling report that have been incorporated into standard MTF estimation work include Fermi edge detection and the newly developed 4th order modified Savitzky-Golay (MSG) interpolation technique. Noise sensitivity had been studied by performing simulations on known noise sources and a sensor model. Extensive investigation was done to characterize multi-resolution ground noise. Finally, angle simulation was tested by using synthetic pulse targets with angles from 2 to 15 degrees, several brightness levels, and different noise levels from both ground targets and imaging system. As a continuing research activity using the developed sensor model, this report was dedicated to MTF estimation via pulse input method characterization using the Fermi edge detection and 4th order MSG interpolation method. The relationship between pulse width and MTF value at Nyquist was studied including error detection and correction schemes. Pulse target angle sensitivity was studied by using synthetic targets angled from 2 to 12 degrees. In this report, from the ground and system noise simulation, a minimum SNR value was suggested for a stable MTF value at Nyquist for the pulse method. Target width error detection and adjustment technique based on a smooth transition of MTF profile is presented, which is specifically applicable only to the pulse method with 3 pixel wide targets.

Objects Grouping for Segmentation of Roads Network in High Resolution Images of Urban Areas

NASA Astrophysics Data System (ADS)

Maboudi, M.; Amini, J.; Hahn, M.

2016-06-01

Updated road databases are required for many purposes such as urban planning, disaster management, car navigation, route planning, traffic management and emergency handling. In the last decade, the improvement in spatial resolution of VHR civilian satellite sensors - as the main source of large scale mapping applications - was so considerable that GSD has become finer than size of common urban objects of interest such as building, trees and road parts. This technological advancement pushed the development of "Object-based Image Analysis (OBIA)" as an alternative to pixel-based image analysis methods. Segmentation as one of the main stages of OBIA provides the image objects on which most of the following processes will be applied. Therefore, the success of an OBIA approach is strongly affected by the segmentation quality. In this paper, we propose a purpose-dependent refinement strategy in order to group road segments in urban areas using maximal similarity based region merging. For investigations with the proposed method, we use high resolution images of some urban sites. The promising results suggest that the proposed approach is applicable in grouping of road segments in urban areas.

A study of SO2 emissions and ground surface displacements at Lastarria volcano, Antofagasta Region, Northern Chile

NASA Astrophysics Data System (ADS)

Krewcun, Lucie G.

Lastarria volcano (Chile) is located at the North-West margin of the 'Lazufre' ground inflation signal (37x45 km2), constantly uplifting at a rate of ˜2.5 cm/year since 1996 (Pritchard and Simons 2002; Froger et al. 2007). The Lastarria volcano has the double interest to be superimposed on a second, smaller-scale inflation signal and to be the only degassing area of the Lazufre signal. In this project, we compared daily SO2 burdens recorded by AURA's OMI mission for 2005-2010 with Ground Surface Displacements (GSD) calculated from the Advanced Synthetic Aperture Radar (ASAR) images for 2003-2010. We found a constant maximum displacement rate of 2.44 cm/year for the period 2003-2007 and 0.80- 0.95 cm/year for the period 2007-2010. Total SO 2 emitted is 67.0 kT for the period 2005-2010, but detection of weak SO2 degassing signals in the Andes remains challenging owing to increased noise in the South Atlantic radiation Anomaly region.

Acoustic accessibility investigation for ultrasound mediated treatment of glycogen storage disease type Ia patients.

PubMed

Wang, Shutao; Raju, Balasundar I; Leyvi, Evgeniy; Weinstein, David A; Seip, Ralf

2011-09-01

Glycogen storage disease type Ia (GSDIa) is caused by an inherited defect in the glucose-6-phosphatase gene. The recent advent of targeted ultrasound-mediated delivery (USMD) of plasmid DNA (pDNA) to the liver in conjunction with microbubbles may provide an alternative treatment option. This study focuses on determining the acoustically accessible liver volume in GSDIa patients using transducer models of various geometries with an image-based geometry-driven approach. Results show that transducers with longer focal lengths and smaller apertures (up to an f/number of 2) are able to access larger liver volumes in GSDIa patients while still being capable of delivering the required ultrasound dose in situ (2.5 MPa peak negative pressure at the focus). With sufficiently large acoustic windows and the ability to use glucose to easily assess efficacy, GSD appears to be a good model for testing USMD as proof of principle as a potential therapy for liver applications in general. Copyright © 2011 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

A pilot study using scripted ventilation conditions to identify key factors affecting indoor pollutant concentration and air exchange rate in a residence.

PubMed

Johnson, Ted; Myers, Jeffrey; Kelly, Thomas; Wisbith, Anthony; Ollison, Will

2004-01-01

A pilot study was conducted using an occupied, single-family test house in Columbus, OH, to determine whether a script-based protocol could be used to obtain data useful in identifying the key factors affecting air-exchange rate (AER) and the relationship between indoor and outdoor concentrations of selected traffic-related air pollutants. The test script called for hourly changes to elements of the test house considered likely to influence air flow and AER, including the position (open or closed) of each window and door and the operation (on/off) of the furnace, air conditioner, and ceiling fans. The script was implemented over a 3-day period (January 30-February 1, 2002) during which technicians collected hourly-average data for AER, indoor, and outdoor air concentrations for six pollutants (benzene, formaldehyde (HCHO), polycyclic aromatic hydrocarbons (PAH), carbon monoxide (CO), nitric oxide (NO), and nitrogen oxides (NO(x))), and selected meteorological variables. Consistent with expectations, AER tended to increase with the number of open exterior windows and doors. The 39 AER values measured during the study when all exterior doors and windows were closed varied from 0.36 to 2.29 h(-1) with a geometric mean (GM) of 0.77 h(-1) and a geometric standard deviation (GSD) of 1.435. The 27 AER values measured when at least one exterior door or window was opened varied from 0.50 to 15.8 h(-1) with a GM of 1.98 h(-1) and a GSD of 1.902. AER was also affected by temperature and wind speed, most noticeably when exterior windows and doors were closed. Results of a series of stepwise linear regression analyses suggest that (1) outdoor pollutant concentration and (2) indoor pollutant concentration during the preceding hour were the "variables of choice" for predicting indoor pollutant concentration in the test house under the conditions of this study. Depending on the pollutant and ventilation conditions, one or more of the following variables produced a small, but significant increase in the explained variance (R(2)-value) of the regression equations: AER, number and location of apertures, wind speed, air-conditioning operation, indoor temperature, outdoor temperature, and relative humidity. The indoor concentrations of CO, PAH, NO, and NO(x) were highly correlated with the corresponding outdoor concentrations. The indoor benzene concentrations showed only moderate correlation with outdoor benzene levels, possibly due to a weak indoor source. Indoor formaldehyde concentrations always exceeded outdoor levels, and the correlation between indoor and outdoor concentrations was not statistically significant, indicating the presence of a strong indoor source.

Wood Dust in Joineries and Furniture Manufacturing: An Exposure Determinant and Intervention Study.

PubMed

Douwes, Jeroen; Cheung, Kerry; Prezant, Bradley; Sharp, Mark; Corbin, Marine; McLean, Dave; 't Mannetje, Andrea; Schlunssen, Vivi; Sigsgaard, Torben; Kromhout, Hans; LaMontagne, Anthony D; Pearce, Neil; McGlothlin, James D

2017-05-01

To assess wood dust exposures and determinants in joineries and furniture manufacturing and to evaluate the efficacy of specific interventions on dust emissions under laboratory conditions. Also, in a subsequent follow-up study in a small sample of joinery workshops, we aimed to develop, implement, and evaluate a cost-effective and practicable intervention to reduce dust exposures. Personal inhalable dust (n = 201) was measured in 99 workers from 10 joineries and 3 furniture-making factories. To assess exposure determinants, full-shift video exposure monitoring (VEM) was conducted in 19 workers and task-based VEM in 32 workers (in 7 joineries and 3 furniture factories). We assessed the efficacy of vacuum extraction on hand tools and the use of vacuum cleaners instead of sweeping and dry wiping under laboratory conditions. These measures were subsequently implemented in three joinery workshops with 'high' (>4 mg m-3) and one with 'low' (<2 mg m-3) baseline exposures. We also included two control workshops (one 'low' and one 'high' exposure workshop) in which no interventions were implemented. Exposures were measured 4 months prior and 4 months following the intervention. Average (geometric means) exposures in joinery and furniture making were 2.5 mg m-3 [geometric standard deviations (GSD) 2.5] and 0.6 mg m-3 (GSD 2.3), respectively. In joinery workers cleaning was associated with a 3.0-fold higher (P < 0.001) dust concentration compared to low exposure tasks (e.g. gluing), while the use of hand tools showed 3.0- to 11.0-fold higher (P < 0.001) exposures. In furniture makers, we found a 5.4-fold higher exposure (P < 0.001) with using a table/circular saw. Laboratory efficiency experiments showed a 10-fold decrease in exposure (P < 0.001) when using a vacuum cleaner. Vacuum extraction on hand tools combined with a downdraft table reduced exposures by 42.5% for routing (P < 0.1) and 85.5% for orbital sanding (P < 0.001). Following intervention measures in joineries, a borderline statistically significant (P < 0.10) reduction in exposure of 30% was found in workshops with 'high' baseline exposures, but no reduction was shown in the workshop with 'low' baseline exposures. Wood dust exposure is high in joinery workers and (to a lesser extent) furniture makers with frequent use of hand tools and cleaning being key drivers of exposure. Vacuum extraction on hand tools and alternative cleaning methods reduced workplace exposures substantially, but may be insufficient to achieve compliance with current occupational exposure limits. © The Author 2017. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

The relation between radon in schools and in dwellings: A case study in a rural region of Southern Serbia.

PubMed

Žunić, Z S; Bossew, P; Bochicchio, F; Veselinovic, N; Carpentieri, C; Venoso, G; Antignani, S; Simovic, R; Ćurguz, Z; Udovicic, V; Stojanovska, Z; Tollefsen, T

2017-02-01

Recognized as a significant health hazard, radon (Rn) has been given increasing attention for years. Surveys of different kinds have been performed in many countries to assess the intensity and the geographical extent of possible Rn problems. Common surveys cover mainly dwellings, the indoor place with highest occupancy, and schools, where people spend a large fraction of their lifetime and which can also be considered exemplary for Rn exposure at workplaces; it has however been observed that relating them is difficult. It was unclear whether residential Rn at a location, or in a region, can be predicted by Rn at a school of that location, or vice versa. To current knowledge, no general rule seems applicable, as few models to describe the relationship between Rn in dwellings and in schools have been developed. In Southern Serbia, a Rn survey in a predominantly rural region was based on measurements in primary schools. The question arose whether or to which degree the results can be considered as indicative or even representative for residential Rn concentrations. To answer the question an additional survey of indoor Rn concentrations in dwellings was initiated, designed and performed in Sokobanja district in 2010-2012 in a manner to be able to detect a relationship if it exists. In the study region, 108 dwellings in 12 villages and towns were selected, with one primary school each. In this paper, we investigate how a relation between Rn in schools and dwellings could be identified and quantified, by developing a model and using experimental data from both the above main and additional surveys. The key criterion is the hypothesis that the relation dwellings - schools, if it exists, is stronger for dwellings closer to a school than for those dwellings further away. We propose methods to test the hypothesis. As result, the hypothesis is corroborated at 95% significance level. More specifically, on town level (typical size about 1 km), the Rn concentration ratio dwelling/school is about 0.8 (geometrical mean), with geometrical standard deviation (GSD) about 1.9. For dwelling and school hypothetically in the same location, the ratio is estimated about 0.7 with GSD about 1.5. We think that the methodology can be applied to structurally similar problems. The results could be used to create "conditional maps" of Rn concentration in dwellings, i.e., for example a map of probabilities that indoor Rn concentrations in dwellings exceed 100 Bq/m 3 , as function of Rn concentration in the local school. Copyright © 2016 Elsevier Ltd. All rights reserved.

UAV-based photogrammetry combination of the elevational outcrop and digital surface models: an example of Sanyi active fault in western Taiwan

NASA Astrophysics Data System (ADS)

Hsieh, Cheng-En; Huang, Wen-Jeng; Chang, Ping-Yu; Lo, Wei

2016-04-01

An unmanned aerial vehicle (UAV) with a digital camera is an efficient tool for geologists to investigate structure patterns in the field. By setting ground control points (GCPs), UAV-based photogrammetry provides high-quality and quantitative results such as a digital surface model (DSM) and orthomosaic and elevational images. We combine the elevational outcrop 3D model and a digital surface model together to analyze the structural characteristics of Sanyi active fault in Houli-Fengyuan area, western Taiwan. Furthermore, we collect resistivity survey profiles and drilling core data in the Fengyuan District in order to build the subsurface fault geometry. The ground sample distance (GSD) of an elevational outcrop 3D model is 3.64 cm/pixel in this study. Our preliminary result shows that 5 fault branches are distributed 500 meters wide on the elevational outcrop and the width of Sanyi fault zone is likely much great than this value. Together with our field observations, we propose a structural evolution model to demonstrate how the 5 fault branches developed. The resistivity survey profiles show that Holocene gravel was disturbed by the Sanyi fault in Fengyuan area.

Space moving target detection and tracking method in complex background

NASA Astrophysics Data System (ADS)

Lv, Ping-Yue; Sun, Sheng-Li; Lin, Chang-Qing; Liu, Gao-Rui

2018-06-01

The background of the space-borne detectors in real space-based environment is extremely complex and the signal-to-clutter ratio is very low (SCR ≈ 1), which increases the difficulty for detecting space moving targets. In order to solve this problem, an algorithm combining background suppression processing based on two-dimensional least mean square filter (TDLMS) and target enhancement based on neighborhood gray-scale difference (GSD) is proposed in this paper. The latter can filter out most of the residual background clutter processed by the former such as cloud edge. Through this procedure, both global and local SCR have obtained substantial improvement, indicating that the target has been greatly enhanced. After removing the detector's inherent clutter region through connected domain processing, the image only contains the target point and the isolated noise, in which the isolated noise could be filtered out effectively through multi-frame association. The proposed algorithm in this paper has been compared with some state-of-the-art algorithms for moving target detection and tracking tasks. The experimental results show that the performance of this algorithm is the best in terms of SCR gain, background suppression factor (BSF) and detection results.

Rapid mapping of landslide disaster using UAV- photogrammetry

NASA Astrophysics Data System (ADS)

Cahyono, A. B.; Zayd, R. A.

2018-03-01

Unmanned Aerial Vehicle (UAV) systems offered many advantages in several mapping applications such as slope mapping, geohazard studies, etc. This study utilizes UAV system for landslide disaster occurred in Jombang Regency, East Java. This study concentrates on type of rotor-wing UAV, that is because rotor wing units are stable and able to capture images easily. Aerial photograph were acquired in the form of strips which followed the procedure of acquiring aerial photograph where taken 60 photos. Secondary data of ground control points using GPS Geodetic and check points established using Total Station technique was used. The digital camera was calibrated using close range photogrammetric software and the recovered camera calibration parameters were then used in the processing of digital images. All the aerial photographs were processed using digital photogrammetric software and the output in the form of orthophoto was produced. The final result shows a 1: 1500 scale orthophoto map from the data processing with SfM algorithm with GSD accuracy of 3.45 cm. And the calculated volume of contour line delineation of 10527.03 m3. The result is significantly different from the result of terrestrial methode equal to 964.67 m3 or 8.4% of the difference of both.

Climate change overruns resilience conferred by temperature-dependent sex determination in sea turtles and threatens their survival.

PubMed

Santidrián Tomillo, Pilar; Genovart, Meritxell; Paladino, Frank V; Spotila, James R; Oro, Daniel

2015-08-01

Temperature-dependent sex determination (TSD) is the predominant form of environmental sex determination (ESD) in reptiles, but the adaptive significance of TSD in this group remains unclear. Additionally, the viability of species with TSD may be compromised as climate gets warmer. We simulated population responses in a turtle with TSD to increasing nest temperatures and compared the results to those of a virtual population with genotypic sex determination (GSD) and fixed sex ratios. Then, we assessed the effectiveness of TSD as a mechanism to maintain populations under climate change scenarios. TSD populations were more resilient to increased nest temperatures and mitigated the negative effects of high temperatures by increasing production of female offspring and therefore, future fecundity. That buffered the negative effect of temperature on the population growth. TSD provides an evolutionary advantage to sea turtles. However, this mechanism was only effective over a range of temperatures and will become inefficient as temperatures rise to levels projected by current climate change models. Projected global warming threatens survival of sea turtles, and the IPCC high gas concentration scenario may result in extirpation of the studied population in 50 years. © 2015 John Wiley & Sons Ltd.

Effects of gestation and transition diets, piglet birth weight, and fasting time on depletion of glycogen pools in liver and 3 muscles of newborn piglets.

PubMed

Theil, P K; Cordero, G; Henckel, P; Puggaard, L; Oksbjerg, N; Sørensen, M T

2011-06-01

The experiment was conducted to assess the effects of maternal nutrition in late gestation on glycogen pools of newborn piglets of different birth weights and to assess how rapidly the glycogen pools in the liver and 3 muscles are mobilized during fasting. Until d 108 of gestation, 48 sows were fed a gestation standard diet (GSD) with low dietary fiber (DF, 17.1%), or 1 of 3 diets with high DF (32.3 to 40.4%) consisting of pectin residue (GPR), potato pulp (GPP), or sugar-beet pulp (GSP). From d 108 until farrowing, sows were fed 1 of 6 transition diets with low or high dietary fat: one group received a standard diet (TSD; control) containing 3% animal fat, another group received the TSD diet + 2.5 g/d of hydroxy-methyl butyrate as topdressing (THB), and 4 other groups received diets with 8% added fat from coconut oil (TCO), sunflower oil (TSO), fish oil (TFO), or 4% octanoic acid + 4% fish oil (TOA). Two piglets per litter (the second and fifth born) were blood sampled, and 1 was killed immediately after birth, whereas the other, depending on the litter, was killed after 12, 24, or 28.5 to 36 h (mean 32.5 h) of fasting. Samples of liver, LM, M. semimembranousus (SM), and M. diaphragm (DP) were collected and analyzed for glycogen concentration. No dietary effects (P > 0.20) on glycogen concentrations in liver, LM, SM, or DP were observed. The weight of the liver was affected by gestation diet (P < 0.05) and was greater in GSD and GSP piglets (36.7 and 36.3 g) than in GPR piglets (32.6 g), and intermediate (33.6 g) in GPP piglets. Liver weight, estimated muscle mass, and glycogen pools (P < 0.001) were affected by birth weight, whereas glycogen concentrations in liver and LM, SM, and DP muscles were not (P > 0.05). Liver weight; glycogen concentrations in liver, LM, SM, and DP; and glycogen pools in liver and muscles decreased (P < 0.001) with increasing duration of fasting, and at 32.5 h of fasting, glycogen concentration was reduced by 80% in liver, 64% in DP, 46% in SM, and 36% in LM. Based on a broken-line model, labile glycogen in SM, a locomotory muscle, was estimated to be depleted after 16.4 h of fasting. In conclusion, piglet size had a major impact on estimated glycogen pools, whereas sow nutrition in late gestation had a minor impact, if any. Furthermore, varying proportions of pools of glycogen present in liver and selected muscles were mobilized, and data indicate that newborn piglets are fatally depleted of energy after 16 h of fasting.

Major to ultra trace element bulk rock analysis of nanoparticulate pressed powder pellets by LA-ICP-MS

NASA Astrophysics Data System (ADS)

Peters, Daniel; Pettke, Thomas

2016-04-01

An efficient, clean procedure for bulk rock major to trace element analysis by 193 nm Excimer LA-ICP-MS analysis of nanoparticulate pressed powder pellets (PPPs) employing a binder is presented. Sample powders are milled in water suspension in a planetary ball mill, reducing average grain size by about one order of magnitude compared to common dry milling protocols. Microcrystalline cellulose (MCC) is employed as a binder, improving the mechanical strength of the PPP and the ablation behaviour, because MCC absorbs 193 nm laser light well. Use of MCC binder allows for producing cohesive pellets of materials that cannot be pelletized in their pure forms, such as quartz powder. Rigorous blank quantification was performed on synthetic quartz treated like rock samples, demonstrating that procedural blanks are irrelevant except for a few elements at the 10 ng g-1 concentration level. The LA-ICP-MS PPP analytical procedure was optimised and evaluated using six different SRM powders (JP-1, UB-N, BCR-2, GSP-2, OKUM, and MUH-1). Calibration based on external standardization using SRM 610, SRM 612, BCR-2G, and GSD-1G glasses allows for evaluation of possible matrix effects during LA-ICP-MS analysis. The data accuracy of the PPP LA-ICP-MS analytical procedure compares well to that achieved for liquid ICP-MS and LA-ICP-MS glass analysis, except for element concentrations below ˜30 ng g-1, where liquid ICP-MS offers more precise data and in part lower limits of detection. Uncertainties on the external reproducibility of LA-ICP-MS PPP element concentrations are of the order of 0.5 to 2 % (1σ standard deviation) for concentrations exceeding ˜1 μg g-1. For lower element concentrations these uncertainties increase to 5-10% or higher when analyte-depending limits of detection (LOD) are approached, and LODs do not significantly differ from glass analysis. Sample homogeneity is demonstrated by the high analytical precision, except for very few elements where grain size effects can rarely still be resolved analytically. Matrix effects are demonstrated for PPP analysis of diverse rock compositions and basalt glass analysis when externally calibrated based on SRM 610 and SRM 612 glasses; employing basalt glass GSD-1G or BCR-2G for external standardisation basically eliminates these problems. Perhaps the most prominent progress of the LA-ICP-MS PPP analytical procedure presented here is the fact that trace elements not commonly analysed, i.e. new, unconventional geochemical tracers, can be measured straightforwardly, including volatile elements, the flux elements Li and B, the chalcophile elements As, Sb, Tl, Bi, and elements that alloy with metal containers employed in conventional glass production approaches. The method presented here thus overcomes many common problems and limitations in analytical geochemistry and is shown to be an efficient alternative for bulk rock trace elements analysis.

Predictors of monoterpene exposure in the Danish furniture industry.

PubMed

Hagström, Katja; Jacobsen, Gitte; Sigsgaard, Torben; Schaumburg, Inger; Erlandsen, Mogens; Schlunssen, Vivi

2012-04-01

Individuals who work with pine in the furniture industry may be exposed to monoterpenes, the most abundant of which are α-pinene, β-pinene, and Δ(3)-carene. Monoterpenes are suspected to cause dermatitis and to harm the respiratory system. An understanding of the predictors of monoterpene exposure is therefore important in preventing these adverse effects. These predictors may include general characteristics of the work environment and specific work operations. We sought to assess the extent to which workers are exposed to monoterpenes and to identify possible predictors of monoterpene exposure in the pine furniture industry in Denmark. Passive measurements of the levels of selected monoterpenes (α-pinene, β-pinene, and Δ(3)-carene) were performed on 161 subjects from 17 pine furniture factories in Viborg County, Denmark; one sample was acquired from each worker. Additionally, wood dust samples were collected from 145 workers. Data on potential predictors of exposure were acquired over the course of the day on which the exposure measurements were recorded and could be assigned to one of four hierarchic ordered levels: worker, machine, department, and factory. In addition to univariate analyses, a mixed model was used to account for imbalances within the data and random variation with each of the hierarchically ordered levels. The geometric mean (GM) monoterpene content observed over the 161 measurements was 7.8 mg m(-3) [geometric standard deviation (GSD): 2.4]; the GM wood dust level over 145 measurements was 0.58 mg m(-3) (GSD: 1.49). None of the measured samples exceeded the occupational exposure limit for terpenes in Denmark (25 ppm, 150 mg m(-3)). In the univariate analyses, half of the predictors tested were found to be significant; the multivariate model indicated that only three of the potential predictors were significant. These were the recirculation of air in rooms used for the processing of wood (a factory level predictor), the presence of a supplementary cold air intake (departmental level), and the operation of a glue press (machine level). However, only one factory of the 17 examined used a supplementary cold air intake, and while very high monoterpene levels were observed there, this may be due to factors other than the supplementary intake. In contrast to the situation with wood dust, we found that the bulk of the variation in the data (65%) was attributable to variability on the factory level, with comparatively little being due to the departmental (16%) and machine (0.5%) levels. The fixed terms in the model accounted for 31.8% of the total variance. The predictors of monoterpenes are not the same as those for wood dust exposure; this has implications for the implementation of preventative measures in factories. In order to decrease monoterpene exposure, efforts should be focused on minimizing the recirculation of air in rooms used for woodworking and on increasing awareness of the importance of effective ventilation and enclosure when operating a glue press.

Development of in-orbit refocusing mechanism for SpaceEye-1 electro-optical payload

NASA Astrophysics Data System (ADS)

Lee, Minwoo; Kim, Jongun; Chang, Jin-Soo; Kang, Myung-Seok

2016-09-01

SpaceEye-1 earth observation satellite, developed by Satrec Initiative Co. Ltd., is a 300 kg scale spacecraft with high resolution electro-optical payload (EOS-D) which performs 1 m GSD, 12 km swath in low earth orbit. Metering structure of EOS-D is manufactured with Carbon Fiber Reinforced Plastic (CFRP). Due to the moisture emission from CFRP metering structure, this spaceborne electro-optical payload undergoes shrinkage after orbit insertion. The shrinkage of metering structure causes change of the distance between primary and secondary mirror. In order to compensate the moisture shrinkage effect, two types of thermal refocusing mechanism were developed, analyzed and applied to EOS-D. Thermal analysis simulating in-orbit thermal condition and thermo-elastic displacement analysis was conducted to calculate the performance of refocusing mechanism. For each EOS-D telescope, analytical refocusing range (displacement change between primary and secondary mirror) was 2.5 um and 3.6 um. Thus, the refocusing mechanism can compensate the dimensional instability of metering structure caused by moisture emission. Furthermore, modal, static and wavefront error analysis was conducted in order to evaluate natural frequency, structural stability and optical performance. As a result, it can be concluded that the refocusing system of EOS-D payload can perform its function in orbit.

Naval EarthMap Observer: overview and data processing

NASA Astrophysics Data System (ADS)

Bowles, Jeffrey H.; Davis, Curtiss O.; Carney, Megan; Clamons, Dean; Gao, Bo-Cai; Gillis, David; Kappus, Mary E.; Lamela, G.; Montes, Marcos J.; Palmadesso, Peter J.; Rhea, J.; Snyder, William A.

1999-12-01

We present an overview of the Naval EarthMap Observer (NEMO) spacecraft and then focus on the processing of NEMO data both on-board the spacecraft and on the ground. The NEMO spacecraft provides for Joint Naval needs and demonstrates the use of hyperspectral imagery for the characterization of the littoral environment and for littoral ocean model development. NEMO is being funded jointly by the U.S. government and commercial partners. The Coastal Ocean Imaging Spectrometer (COIS) is the primary instrument on the NEMO and covers the spectral range from 400 to 2500 nm at 10-nm resolution with either 30 or 60 m work GSD. The hyperspectral data is processed on-board the NEMO using NRL's Optical Real-time Automated Spectral Identification System (ORASIS) algorithm that provides for real time analysis, feature extraction and greater than 10:1 data compression. The high compression factor allows for ground coverage of greater than 106 km2/day. Calibration of the sensor is done with a combination of moon imaging, using an onboard light source and vicarious calibration using a number of earth sites being monitored for that purpose. The data will be atmospherically corrected using ATREM. Algorithms will also be available to determine water clarity, bathymetry and bottom type.

RapidEye constellation relative radiometric accuracy measurement using lunar images

NASA Astrophysics Data System (ADS)

Steyn, Joe; Tyc, George; Beckett, Keith; Hashida, Yoshi

2009-09-01

The RapidEye constellation includes five identical satellites in Low Earth Orbit (LEO). Each satellite has a 5-band (blue, green, red, red-edge and near infrared (NIR)) multispectral imager at 6.5m GSD. A three-axes attitude control system allows pointing the imager of each satellite at the Moon during lunations. It is therefore possible to image the Moon from near identical viewing geometry within a span of 80 minutes with each one of the imagers. Comparing the radiometrically corrected images obtained from each band and each satellite allows a near instantaneous relative radiometric accuracy measurement and determination of relative gain changes between the five imagers. A more traditional terrestrial vicarious radiometric calibration program has also been completed by MDA on RapidEye. The two components of this program provide for spatial radiometric calibration ensuring that detector-to-detector response remains flat, while a temporal radiometric calibration approach has accumulated images of specific dry dessert calibration sites. These images are used to measure the constellation relative radiometric response and make on-ground gain and offset adjustments in order to maintain the relative accuracy of the constellation within +/-2.5%. A quantitative comparison between the gain changes measured by the lunar method and the terrestrial temporal radiometric calibration method is performed and will be presented.

A bounding estimate of neutron dose based on measured photon dose around single pass reactors at the Hanford site.

PubMed

Taulbee, Timothy D; Glover, Samuel E; Macievic, Gregory V; Hunacek, Mickey; Smith, Cheryl; DeBord, Gary W; Morris, Donald; Fix, Jack

2010-07-01

Neutron and photon radiation survey records have been used to evaluate and develop a neutron to photon (NP) ratio to reconstruct neutron doses to workers around Hanford's single pass reactors that operated from 1945 to 1972. A total of 5,773 paired neutron and photon measurements extracted from 57 boxes of survey records were used in the development of the NP ratio. The development of the NP ratio enables the use of the recorded dose from an individual's photon dosimeter badge to be used to estimate the unmonitored neutron dose. The Pearson rank correlation between the neutron and photon measurements was 0.71. The NP ratio best fit a lognormal distribution with a geometric mean (GM) of 0.8, a geometric standard deviation (GSD) of 2.95, and the upper 95 th % of this distribution was 4.75. An estimate of the neutron dose based on this NP ratio is considered bounding due to evidence that up to 70% of the total photon exposure received by workers around the single pass reactors occurs during shutdown maintenance and refueling activities when there is no significant neutron exposure. Thus when this NP ratio is applied to the total measured photon dose from an individual film badge dosimeter, the resulting neutron dose is considered bounded.

A Backpack-Mounted Omnidirectional Camera with Off-the-Shelf Navigation Sensors for Mobile Terrestrial Mapping: Development and Forest Application

PubMed Central

Prol, Fabricio dos Santos; El Issaoui, Aimad; Hakala, Teemu

2018-01-01

The use of Personal Mobile Terrestrial System (PMTS) has increased considerably for mobile mapping applications because these systems offer dynamic data acquisition with ground perspective in places where the use of wheeled platforms is unfeasible, such as forests and indoor buildings. PMTS has become more popular with emerging technologies, such as miniaturized navigation sensors and off-the-shelf omnidirectional cameras, which enable low-cost mobile mapping approaches. However, most of these sensors have not been developed for high-accuracy metric purposes and therefore require rigorous methods of data acquisition and data processing to obtain satisfactory results for some mapping applications. To contribute to the development of light, low-cost PMTS and potential applications of these off-the-shelf sensors for forest mapping, this paper presents a low-cost PMTS approach comprising an omnidirectional camera with off-the-shelf navigation systems and its evaluation in a forest environment. Experimental assessments showed that the integrated sensor orientation approach using navigation data as the initial information can increase the trajectory accuracy, especially in covered areas. The point cloud generated with the PMTS data had accuracy consistent with the Ground Sample Distance (GSD) range of omnidirectional images (3.5–7 cm). These results are consistent with those obtained for other PMTS approaches. PMID:29522467

Airborne multidimensional integrated remote sensing system

NASA Astrophysics Data System (ADS)

Xu, Weiming; Wang, Jianyu; Shu, Rong; He, Zhiping; Ma, Yanhua

2006-12-01

In this paper, we present a kind of airborne multidimensional integrated remote sensing system that consists of an imaging spectrometer, a three-line scanner, a laser ranger, a position & orientation subsystem and a stabilizer PAV30. The imaging spectrometer is composed of two sets of identical push-broom high spectral imager with a field of view of 22°, which provides a field of view of 42°. The spectral range of the imaging spectrometer is from 420nm to 900nm, and its spectral resolution is 5nm. The three-line scanner is composed of two pieces of panchromatic CCD and a RGB CCD with 20° stereo angle and 10cm GSD(Ground Sample Distance) with 1000m flying height. The laser ranger can provide height data of three points every other four scanning lines of the spectral imager and those three points are calibrated to match the corresponding pixels of the spectral imager. The post-processing attitude accuracy of POS/AV 510 used as the position & orientation subsystem, which is the aerial special exterior parameters measuring product of Canadian Applanix Corporation, is 0.005° combined with base station data. The airborne multidimensional integrated remote sensing system was implemented successfully, performed the first flying experiment on April, 2005, and obtained satisfying data.

Skeletal muscle disorders of glycogenolysis and glycolysis.

PubMed

Godfrey, Richard; Quinlivan, Ros

2016-07-01

Skeletal muscle disorders of glycogenolysis and glycolysis account for most of the conditions collectively termed glycogen storage diseases (GSDs). These disorders are rare (incidence 1 in 20,000-43,000 live births), and are caused by autosomal or X-linked recessive mutations that result in a specific enzyme deficiency, leading to the inability to utilize muscle glycogen as an energy substrate. McArdle disease (GSD V) is the most common of these disorders, and is caused by mutations in the gene encoding muscle glycogen phosphorylase. Symptoms of McArdle disease and most other related GSDs include exercise intolerance, muscle contracture, acute rhabdomyolysis, and risk of acute renal failure. Older patients may exhibit muscle wasting and weakness involving the paraspinal muscles and shoulder girdle. For patients with these conditions, engaging with exercise is likely to be beneficial. Diagnosis is frequently delayed owing to the rarity of the conditions and lack of access to appropriate investigations. A few randomized clinical trials have been conducted, some focusing on dietary modification, although the quality of the evidence is low and no specific recommendations can yet be made. The development of EUROMAC, an international registry for these disorders, should improve our knowledge of their natural histories and provide a platform for future clinical trials.

Particle sizing of pharmaceutical aerosols via direct imaging of particle settling velocities.

PubMed

Fishler, Rami; Verhoeven, Frank; de Kruijf, Wilbur; Sznitman, Josué

2018-02-15

We present a novel method for characterizing in near real-time the aerodynamic particle size distributions from pharmaceutical inhalers. The proposed method is based on direct imaging of airborne particles followed by a particle-by-particle measurement of settling velocities using image analysis and particle tracking algorithms. Due to the simplicity of the principle of operation, this method has the potential of circumventing potential biases of current real-time particle analyzers (e.g. Time of Flight analysis), while offering a cost effective solution. The simple device can also be constructed in laboratory settings from off-the-shelf materials for research purposes. To demonstrate the feasibility and robustness of the measurement technique, we have conducted benchmark experiments whereby aerodynamic particle size distributions are obtained from several commercially-available dry powder inhalers (DPIs). Our measurements yield size distributions (i.e. MMAD and GSD) that are closely in line with those obtained from Time of Flight analysis and cascade impactors suggesting that our imaging-based method may embody an attractive methodology for rapid inhaler testing and characterization. In a final step, we discuss some of the ongoing limitations of the current prototype and conceivable routes for improving the technique. Copyright © 2017 Elsevier B.V. All rights reserved.

Phylo-VISTA: Interactive visualization of multiple DNA sequence alignments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shah, Nameeta; Couronne, Olivier; Pennacchio, Len A.

The power of multi-sequence comparison for biological discovery is well established. The need for new capabilities to visualize and compare cross-species alignment data is intensified by the growing number of genomic sequence datasets being generated for an ever-increasing number of organisms. To be efficient these visualization algorithms must support the ability to accommodate consistently a wide range of evolutionary distances in a comparison framework based upon phylogenetic relationships. Results: We have developed Phylo-VISTA, an interactive tool for analyzing multiple alignments by visualizing a similarity measure for multiple DNA sequences. The complexity of visual presentation is effectively organized using a frameworkmore » based upon interspecies phylogenetic relationships. The phylogenetic organization supports rapid, user-guided interspecies comparison. To aid in navigation through large sequence datasets, Phylo-VISTA leverages concepts from VISTA that provide a user with the ability to select and view data at varying resolutions. The combination of multiresolution data visualization and analysis, combined with the phylogenetic framework for interspecies comparison, produces a highly flexible and powerful tool for visual data analysis of multiple sequence alignments. Availability: Phylo-VISTA is available at http://www-gsd.lbl. gov/phylovista. It requires an Internet browser with Java Plugin 1.4.2 and it is integrated into the global alignment program LAGAN at http://lagan.stanford.edu« less

A Ground Systems Architecture Transition for a Distributed Operations System

NASA Technical Reports Server (NTRS)

Sellers, Donna; Pitts, Lee; Bryant, Barry

2003-01-01

The Marshall Space Flight Center (MSFC) Ground Systems Department (GSD) recently undertook an architecture change in the product line that serves the ISS program. As a result, the architecture tradeoffs between data system product lines that serve remote users versus those that serve control center flight control teams were explored extensively. This paper describes the resulting architecture that will be used in the International Space Station (ISS) payloads program, and the resulting functional breakdown of the products that support this architecture. It also describes the lessons learned from the path that was followed, as a migration of products cause the need to reevaluate the allocation of functions across the architecture. The result is a set of innovative ground system solutions that is scalable so it can support facilities of wide-ranging sizes, from a small site up to large control centers. Effective use of system automation, custom components, design optimization for data management, data storage, data transmissions, and advanced local and wide area networking architectures, plus the effective use of Commercial-Off-The-Shelf (COTS) products, provides flexible Remote Ground System options that can be tailored to the needs of each user. This paper offers a description of the efficiency and effectiveness of the Ground Systems architectural options that have been implemented, and includes successful implementation examples and lessons learned.

Indicator Species Population Monitoring in Antarctica with Uav

NASA Astrophysics Data System (ADS)

Zmarz, A.; Korczak-Abshire, M.; Storvold, R.; Rodzewicz, M.; Kędzierska, I.

2015-08-01

A program to monitor bird and pinniped species in the vicinity of Arctowski Station, King George Island, South Shetlands, Antarctica, has been conducted over the past 38 years. Annual monitoring of these indicator species includes estimations of breeding population sizes of three Pygoscelis penguin species: Adélie, gentoo and chinstrap. Six penguin colonies situated on the western shores of two bays: Admiralty and King George are investigated. To study changes in penguin populations Unmanned Aerial Vehicles were used for the first time in the 2014/15 austral summer season. During photogrammetric flights the high-resolution images of eight penguin breeding colonies were taken. Obtained high resolution images were used for estimation of breeding population size and compared with the results of measurements taken at the same time from the ground. During this Antarctic expedition eight successful photogrammetry missions (total distance 1500 km) were performed. Images were taken with digital SLR Canon 700D, Nikon D5300, Nikon D5100 with a 35mm objective lens. Flights altitude at 350 - 400 AGL, allowed images to be taken with a resolution GSD (ground sample distance) less than 5 cm. The Image J software analysis method was tested to provide automatic population estimates from obtained images. The use of UAV for monitoring of indicator species, enabled data acquisition from areas inaccessible by ground methods.

Genome-wide association studies for multiple diseases of the German Shepherd Dog

PubMed Central

Tsai, Kate L.; Noorai, Rooksana E.; Starr-Moss, Alison N.; Quignon, Pascale; Rinz, Caitlin J.; Ostrander, Elaine A.; Steiner, Jörg M.; Murphy, Keith E.

2012-01-01

The German Shepherd Dog (GSD) is a popular working and companion breed for which over 50 hereditary diseases have been documented. Herein, SNP profiles for 197 GSDs were generated using the Affymetrix v2 canine SNP array for a genome-wide association study to identify loci associated with four diseases: pituitary dwarfism, degenerative myelopathy (DM), congenital megaesophagus (ME), and pancreatic acinar atrophy (PAA). A locus on Chr 9 is strongly associated with pituitary dwarfism and is proximal to a plausible candidate gene, LHX3. Results for DM confirm a major locus encompassing SOD1, in which an associated point mutation was previously identified, but do not suggest modifier loci. Several SNPs on Chr 12 are associated with ME and a 4.7 Mb haplotype block is present in affected dogs. Analysis of additional ME cases for a SNP within the haplotype provides further support for this association. Results for PAA indicate more complex genetic underpinnings. Several regions on multiple chromosomes reach genome-wide significance. However, no major locus is apparent and only two associated haplotype blocks, on Chrs 7 and 12 are observed. These data suggest that PAA may be governed by multiple loci with small effects, or it may be a heterogeneous disorder. PMID:22105877

Generation, Validation, and Application of Abundance Map Reference Data for Spectral Unmixing

NASA Astrophysics Data System (ADS)

Williams, McKay D.

Reference data ("ground truth") maps traditionally have been used to assess the accuracy of imaging spectrometer classification algorithms. However, these reference data can be prohibitively expensive to produce, often do not include sub-pixel abundance estimates necessary to assess spectral unmixing algorithms, and lack published validation reports. Our research proposes methodologies to efficiently generate, validate, and apply abundance map reference data (AMRD) to airborne remote sensing scenes. We generated scene-wide AMRD for three different remote sensing scenes using our remotely sensed reference data (RSRD) technique, which spatially aggregates unmixing results from fine scale imagery (e.g., 1-m Ground Sample Distance (GSD)) to co-located coarse scale imagery (e.g., 10-m GSD or larger). We validated the accuracy of this methodology by estimating AMRD in 51 randomly-selected 10 m x 10 m plots, using seven independent methods and observers, including field surveys by two observers, imagery analysis by two observers, and RSRD using three algorithms. Results indicated statistically-significant differences between all versions of AMRD, suggesting that all forms of reference data need to be validated. Given these significant differences between the independent versions of AMRD, we proposed that the mean of all (MOA) versions of reference data for each plot and class were most likely to represent true abundances. We then compared each version of AMRD to MOA. Best case accuracy was achieved by a version of imagery analysis, which had a mean coverage area error of 2.0%, with a standard deviation of 5.6%. One of the RSRD algorithms was nearly as accurate, achieving a mean error of 3.0%, with a standard deviation of 6.3%, showing the potential of RSRD-based AMRD generation. Application of validated AMRD to specific coarse scale imagery involved three main parts: 1) spatial alignment of coarse and fine scale imagery, 2) aggregation of fine scale abundances to produce coarse scale imagery-specific AMRD, and 3) demonstration of comparisons between coarse scale unmixing abundances and AMRD. Spatial alignment was performed using our scene-wide spectral comparison (SWSC) algorithm, which aligned imagery with accuracy approaching the distance of a single fine scale pixel. We compared simple rectangular aggregation to coarse sensor point spread function (PSF) aggregation, and found that the PSF approach returned lower error, but that rectangular aggregation more accurately estimated true abundances at ground level. We demonstrated various metrics for comparing unmixing results to AMRD, including mean absolute error (MAE) and linear regression (LR). We additionally introduced reference data mean adjusted MAE (MA-MAE), and reference data confidence interval adjusted MAE (CIA-MAE), which account for known error in the reference data itself. MA-MAE analysis indicated that fully constrained linear unmixing of coarse scale imagery across all three scenes returned an error of 10.83% per class and pixel, with regression analysis yielding a slope = 0.85, intercept = 0.04, and R2 = 0.81. Our reference data research has demonstrated a viable methodology to efficiently generate, validate, and apply AMRD to specific examples of airborne remote sensing imagery, thereby enabling direct quantitative assessment of spectral unmixing performance.

Characterizing pollutant emissions from mosquito repellents incenses and implications in risk assessment of human health.

PubMed

Wang, Lina; Zheng, Xinran; Stevanovic, Svetlana; Xiang, Zhiyuan; Liu, Jing; Shi, Huiwen; Liu, Jing; Yu, Mingzhou; Zhu, Chun

2018-01-01

Mosquito-repellent incense is one of the most popular products used for dispelling mosquitos during summer in China. It releases large amounts of particulate and gaseous pollutants which constitute a potential hazard to human health. We conducted chamber experiment to characterize major pollutants from three types of mosquito-repellent incenses, further assessed the size-fractionated deposition in human respiratory system, and evaluated the indoor removing efficiency by fresh air. Results showed that the released pollutant concentrations were greater than permissible levels in regulations in GB3095-2012, as well as suggested by the World Health Organization (WHO). Formaldehyde accounted for 10-20% of the total amount of pollutants. Fine particles dominated in the total particulate concentrations. Geometric standard deviation (GSD) of particle number size distributions was in the range of 1.45-1.93. Count median diameter (CMD) ranged from 100 to 500 nm. Emission rates, burning rates and emission factors of both particulate and gaseous pollutants were compared and discussed. The deposition fractions in pulmonary airway from the disc solid types reached up to 52.7% of the total deposition, and the largest deposition appeared on juvenile group. Computational Fluid Dynamics (CFD) modellings indicated air-conditioner on and windows closed was the worst case. The highest concentration was 180-200 times over the standard limit. Copyright © 2017 Elsevier Ltd. All rights reserved.

Edge method for on-orbit defocus assessment.

PubMed

Viallefont-Robinet, Françoise

2010-09-27

In the earth observation domain, two classes of sensors may be distinguished: a class for which sensor performances are driven by radiometric accuracy of the images and a class for which sensor performances are driven by spatial resolution. In this latter case, as spatial resolution depends on the triplet constituted by the Ground Sampling Distance (GSD), Modulation Transfer Function (MTF), and Signal to Noise Ratio (SNR), refocusing, acting as an MTF improvement, is very important. Refocusing is not difficult by itself as far as the on-board mechanism is reliable. The difficulty is on the defocus assessment side. Some methods such as those used for the SPOT family rely on the ability of the satellite to image the same landscape with two focusing positions. This can be done with a bi-sensor configuration, with adequate focal plane, or with the satellite agility. A new generation of refocusing mechanism will be taken aboard Pleiades. As the speed of this mechanism will be much slower than the speed of the older generation, it won't be possible, despite the agility of the satellite, to image the same landscape with two focusing positions on the same orbit. That's why methods relying on MTF measurement with edge method have been studied. This paper describes the methods and the work done to assess the defocus measurement accuracy in the Pleiades context.

Adaptive optics using a MEMS deformable mirror for a segmented mirror telescope

NASA Astrophysics Data System (ADS)

Miyamura, Norihide

2017-09-01

For small satellite remote sensing missions, a large aperture telescope more than 400mm is required to realize less than 1m GSD observations. However, it is difficult or expensive to realize the large aperture telescope using a monolithic primary mirror with high surface accuracy. A segmented mirror telescope should be studied especially for small satellite missions. Generally, not only high accuracy of optical surface but also high accuracy of optical alignment is required for large aperture telescopes. For segmented mirror telescopes, the alignment is more difficult and more important. For conventional systems, the optical alignment is adjusted before launch to achieve desired imaging performance. However, it is difficult to adjust the alignment for large sized optics in high accuracy. Furthermore, thermal environment in orbit and vibration in a launch vehicle cause the misalignments of the optics. We are developing an adaptive optics system using a MEMS deformable mirror for an earth observing remote sensing sensor. An image based adaptive optics system compensates the misalignments and wavefront aberrations of optical elements using the deformable mirror by feedback of observed images. We propose the control algorithm of the deformable mirror for a segmented mirror telescope by using of observed image. The numerical simulation results and experimental results show that misalignment and wavefront aberration of the segmented mirror telescope are corrected and image quality is improved.

Glycogen Storage Disease Type Ia: Linkage of Glucose, Glycogen, Lactic Acid, Triglyceride, and Uric Acid Metabolism

PubMed Central

Sever, Sakine; Weinstein, David A.; Wolfsdorf, Joseph I.; Gedik, Reyhan; Schaefer, Ernst J.

2013-01-01

Case Summary A female presented in infancy with hypotonia, undetectable serum glucose, lactic acidosis, and triglycerides > 5,000 mg/dl. The diagnosis of type 1A glycogen storage disease (GSD) was made by liver biopsy that showed increased glycogen and absent glucose-6-phosphatase enzyme activity. She was treated with dextrose feeding, which was replaced by frequent cornstarch feeding, with improvement of her metabolic parameters. At age 18 years she had marked hypertriglyceridemia (3,860 mg/dl) and eruptive xanthomas, and was treated with fenofibrate, atorvastatin, and fish oil. At age 29 years she was noted to have multiple liver adenomas, severe anemia, and hyperuricemia. Aggressive cornstarch therapy was commenced with a goal of maintaining her blood glucose levels > 75 mg/dl and lactate levels < 2 mmol/L. After 15 months on this regimen, her lipids levels (measured in mg/dl) off all medications were: total cholesterol 222, triglycerides 179, high density lipoprotein cholesterol 32, and calculated low density lipoprotein cholesterol 154. Her weight was stable with a body mass index of 24.8 kg/m2. Her liver adenomas had decreased in size, and her anemia and hyperuricemia had improved. She was homozygous for the R83C missense mutation in G6PC. Our data indicate that optimized metabolic control to maintain blood glucose levels > 75 mg/dl is critical in the management of this disease. PMID:23312056

Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy

PubMed Central

German, Christopher L.; Gudheti, Manasa V.; Fleckenstein, Annette E.; Jorgensen, Erik M.

2018-01-01

Localization microscopy techniques – such as photoactivation localization microscopy (PALM), fluorescent PALM (FPALM), ground state depletion (GSD), and stochastic optical reconstruction microscopy (STORM) – provide the highest precision for single molecule localization currently available. However, localization microscopy has been largely limited to cell cultures due to the difficulties that arise in imaging thicker tissue sections. Sample fixation and antibody staining, background fluorescence, fluorophore photoinstability, light scattering in thick sections, and sample movement create significant challenges for imaging intact tissue. We have developed a sample preparation and image acquisition protocol to address these challenges in rat brain slices. The sample preparation combined multiple fixation steps, saponin permeabilization, and tissue clarification. Together, these preserve intracellular structures, promote antibody penetration, reduce background fluorescence and light scattering, and allow acquisition of images deep in a 30 μm thick slice. Image acquisition challenges were resolved by overlaying samples with a permeable agarose pad and custom-built stainless steel imaging adapter, and sealing the imaging chamber. This approach kept slices flat, immobile, bathed in imaging buffer, and prevented buffer oxidation during imaging. Using this protocol, we consistently obtained single molecule localizations of synaptic vesicle and active zone proteins in three-dimensions within individual synaptic terminals of the striatum in rat brain slices. These techniques may be easily adapted to the preparation and imaging of other tissues, substantially broadening the application of super-resolution imaging. PMID:28924666

Dobson space telescope: development of an optical payload of the next generation

NASA Astrophysics Data System (ADS)

Segert, Tom; Danziger, Björn; Gork, Daniel; Lieder, Matthias

2017-11-01

The Dobson Space Telescope (DST) is a research project of the Department of Astronautics at the TUBerlin. For Development and commercialisation there is a close cooperation with the network of the Berlin Space Industry (RIBB). Major Partner is the Astro- und Feinwerktechnik Adlershof GmbH a specialist for space structures and head of the industry consortia which built the DLR BIRD micro satellite. The aim of the project is to develop a new type of deployable telescope that can overcome the mass and volume limitations of small satellites. With the DST payload micro satellites of the 100kg class will be able to carry 50cm main mirror diameter optics (→ 1m GSD). Basis of this technology is the fact that a telescope is mainly empty space between the optical elements. To fold down the telescope during launch and to undfold it after the satellite reached its orbit can save 70% of payload volume and 50% of payload mass. Since these advantages continue along the value added chain DST is of highest priority for the next generation of commercial EO micro satellites. Since 2002 the key technologies for DST have been developed in test benches in Labs of TU-Berlin and were tested on board a ESA parabolic flight campaign in 2005. The development team at TU-Berlin currently prepares the foundation of a start-up company for further development and commercialisation of DST.

Approach for Improving the Integrated Sensor Orientation

NASA Astrophysics Data System (ADS)

Mitishita, E.; Ercolin Filho, L.; Graça, N.; Centeno, J.

2016-06-01

The direct determination of exterior orientation parameters (EOP) of aerial images via integration of the Inertial Measurement Unit (IMU) and GPS is often used in photogrammetric mapping nowadays. The accuracies of the EOP depend on the accurate parameters related to sensors mounting when the job is performed (offsets of the IMU relative to the projection centre and the angles of boresigth misalignment between the IMU and the photogrammetric coordinate system). In principle, when the EOP values do not achieve the required accuracies for the photogrammetric application, the approach, known as Integrated Sensor Orientation (ISO), is used to refine the direct EOP. ISO approach requires accurate Interior Orientation Parameters (IOP) and standard deviation of the EOP under flight condition. This paper investigates the feasibility of use the in situ camera calibration to obtain these requirements. The camera calibration uses a small sub block of images, extracted from the entire block. A digital Vexcel UltraCam XP camera connected to APPLANIX POS AVTM system was used to get two small blocks of images that were use in this study. The blocks have different flight heights and opposite flight directions. The proposed methodology improved significantly the vertical and horizontal accuracies of the 3D point intersection. Using a minimum set of control points, the horizontal and vertical accuracies achieved nearly one image pixel of resolution on the ground (GSD). The experimental results are shown and discussed.

An automated, open-source pipeline for mass production of digital elevation models (DEMs) from very-high-resolution commercial stereo satellite imagery

NASA Astrophysics Data System (ADS)

Shean, David E.; Alexandrov, Oleg; Moratto, Zachary M.; Smith, Benjamin E.; Joughin, Ian R.; Porter, Claire; Morin, Paul

2016-06-01

We adapted the automated, open source NASA Ames Stereo Pipeline (ASP) to generate digital elevation models (DEMs) and orthoimages from very-high-resolution (VHR) commercial imagery of the Earth. These modifications include support for rigorous and rational polynomial coefficient (RPC) sensor models, sensor geometry correction, bundle adjustment, point cloud co-registration, and significant improvements to the ASP code base. We outline a processing workflow for ˜0.5 m ground sample distance (GSD) DigitalGlobe WorldView-1 and WorldView-2 along-track stereo image data, with an overview of ASP capabilities, an evaluation of ASP correlator options, benchmark test results, and two case studies of DEM accuracy. Output DEM products are posted at ˜2 m with direct geolocation accuracy of <5.0 m CE90/LE90. An automated iterative closest-point (ICP) co-registration tool reduces absolute vertical and horizontal error to <0.5 m where appropriate ground-control data are available, with observed standard deviation of ˜0.1-0.5 m for overlapping, co-registered DEMs (n = 14, 17). While ASP can be used to process individual stereo pairs on a local workstation, the methods presented here were developed for large-scale batch processing in a high-performance computing environment. We are leveraging these resources to produce dense time series and regional mosaics for the Earth's polar regions.

Automatic Hotspot and Sun Glint Detection in UAV Multispectral Images

PubMed Central

Ortega-Terol, Damian; Ballesteros, Rocio

2017-01-01

Last advances in sensors, photogrammetry and computer vision have led to high-automation levels of 3D reconstruction processes for generating dense models and multispectral orthoimages from Unmanned Aerial Vehicle (UAV) images. However, these cartographic products are sometimes blurred and degraded due to sun reflection effects which reduce the image contrast and colour fidelity in photogrammetry and the quality of radiometric values in remote sensing applications. This paper proposes an automatic approach for detecting sun reflections problems (hotspot and sun glint) in multispectral images acquired with an Unmanned Aerial Vehicle (UAV), based on a photogrammetric strategy included in a flight planning and control software developed by the authors. In particular, two main consequences are derived from the approach developed: (i) different areas of the images can be excluded since they contain sun reflection problems; (ii) the cartographic products obtained (e.g., digital terrain model, orthoimages) and the agronomical parameters computed (e.g., normalized vegetation index-NVDI) are improved since radiometric defects in pixels are not considered. Finally, an accuracy assessment was performed in order to analyse the error in the detection process, getting errors around 10 pixels for a ground sample distance (GSD) of 5 cm which is perfectly valid for agricultural applications. This error confirms that the precision in the detection of sun reflections can be guaranteed using this approach and the current low-cost UAV technology. PMID:29036930

Nucleon scalar and tensor charges using lattice QCD simulations at the physical value of the pion mass

NASA Astrophysics Data System (ADS)

Alexandrou, C.; Constantinou, M.; Dimopoulos, P.; Frezzotti, R.; Hadjiyiannakou, K.; Jansen, K.; Kallidonis, C.; Kostrzewa, B.; Koutsou, G.; Mangin-Brinet, M.; Vaquero Avilès-Casco, A.; Wenger, U.

2017-06-01

We present results on the light, strange and charm nucleon scalar and tensor charges from lattice QCD, using simulations with Nf=2 flavors of twisted mass clover-improved fermions with a physical value of the pion mass. Both connected and disconnected contributions are included, enabling us to extract the isoscalar, strange and charm charges for the first time directly at the physical point. Furthermore, the renormalization is computed nonperturbatively for both isovector and isoscalar quantities. We investigate excited state effects by analyzing several sink-source time separations and by employing a set of methods to probe ground state dominance. Our final results for the scalar charges are gSu=5.20 (42 )(15 )(12 ), gSd=4.27 (26 )(15 )(12 ), gSs=0.33 (7 )(1 )(4 ), and gSc=0.062 (13 )(3 )(5 ) and for the tensor charges gTu=0.794 (16 )(2 )(13 ), gTd=-0.210 (10 )(2 )(13 ), gTs=0.00032 (24 )(0 ), and gTc=0.00062 (85 )(0 ) in the MS ¯ scheme at 2 GeV. The first error is statistical, the second is the systematic error due to the renormalization and the third the systematic arising from estimating the contamination due to the excited states, when our data are precise enough to probe the first excited state.

Characterisation of adhesional properties of lactose carriers using atomic force microscopy.

PubMed

Louey, M D; Mulvaney, P; Stewart, P J

2001-06-01

The atomic force microscopy (AFM) colloid probe technique was investigated as a method for the characterisation of adhesional properties of pharmaceutical powder surfaces. Lactose carriers used in dry powder inhaler (DPI) formulations were chosen for investigation since adhesion between the carrier surface and drug particles has been proposed to affect the dispersion of drug particles. Individual adhesion forces were determined by measuring the detachment forces in air between the colloid probe and the lactose particle surface. The colloid probe consisted of a silica sphere (10 microm diameter) attached to a V-shaped silicon nitride cantilever (spring constant, k=0.42 N/m). Adhesion forces were calculated from individual force-distance curves using Hooke's Law. Individual forces measured at various adhesion sites were observed to be reproducible and stable over 10 min (coefficient of variation, CV below 5%). The adhesion force distribution determined from measurements at multiple sites (n>50) on each sample followed a log-normal relationship (regression coefficient, r(2) ranged between 0.95 and 0.99). This enabled characterisation in terms of the geometric mean adhesion force and a geometric standard deviation (GSD). Significant differences (P<0.001) in adhesion force were observed between samples, ranging from 37.47+/-1.95 to 117.48+/-2.20 nN. This study demonstrates the suitability of AFM as sensitive technique for the characterisation of adhesional properties of pharmaceutical particles.

Automatic Hotspot and Sun Glint Detection in UAV Multispectral Images.

PubMed

Ortega-Terol, Damian; Hernandez-Lopez, David; Ballesteros, Rocio; Gonzalez-Aguilera, Diego

2017-10-15

Last advances in sensors, photogrammetry and computer vision have led to high-automation levels of 3D reconstruction processes for generating dense models and multispectral orthoimages from Unmanned Aerial Vehicle (UAV) images. However, these cartographic products are sometimes blurred and degraded due to sun reflection effects which reduce the image contrast and colour fidelity in photogrammetry and the quality of radiometric values in remote sensing applications. This paper proposes an automatic approach for detecting sun reflections problems (hotspot and sun glint) in multispectral images acquired with an Unmanned Aerial Vehicle (UAV), based on a photogrammetric strategy included in a flight planning and control software developed by the authors. In particular, two main consequences are derived from the approach developed: (i) different areas of the images can be excluded since they contain sun reflection problems; (ii) the cartographic products obtained (e.g., digital terrain model, orthoimages) and the agronomical parameters computed (e.g., normalized vegetation index-NVDI) are improved since radiometric defects in pixels are not considered. Finally, an accuracy assessment was performed in order to analyse the error in the detection process, getting errors around 10 pixels for a ground sample distance (GSD) of 5 cm which is perfectly valid for agricultural applications. This error confirms that the precision in the detection of sun reflections can be guaranteed using this approach and the current low-cost UAV technology.

Slic Superpixels for Object Delineation from Uav Data

NASA Astrophysics Data System (ADS)

Crommelinck, S.; Bennett, R.; Gerke, M.; Koeva, M. N.; Yang, M. Y.; Vosselman, G.

2017-08-01

Unmanned aerial vehicles (UAV) are increasingly investigated with regard to their potential to create and update (cadastral) maps. UAVs provide a flexible and low-cost platform for high-resolution data, from which object outlines can be accurately delineated. This delineation could be automated with image analysis methods to improve existing mapping procedures that are cost, time and labor intensive and of little reproducibility. This study investigates a superpixel approach, namely simple linear iterative clustering (SLIC), in terms of its applicability to UAV data. The approach is investigated in terms of its applicability to high-resolution UAV orthoimages and in terms of its ability to delineate object outlines of roads and roofs. Results show that the approach is applicable to UAV orthoimages of 0.05 m GSD and extents of 100 million and 400 million pixels. Further, the approach delineates the objects with the high accuracy provided by the UAV orthoimages at completeness rates of up to 64 %. The approach is not suitable as a standalone approach for object delineation. However, it shows high potential for a combination with further methods that delineate objects at higher correctness rates in exchange of a lower localization quality. This study provides a basis for future work that will focus on the incorporation of multiple methods for an interactive, comprehensive and accurate object delineation from UAV data. This aims to support numerous application fields such as topographic and cadastral mapping.

A gatekeeping procedure to test a primary and a secondary endpoint in a group sequential design with multiple interim looks.

PubMed

Tamhane, Ajit C; Gou, Jiangtao; Jennison, Christopher; Mehta, Cyrus R; Curto, Teresa

2018-03-01

Glimm et al. (2010) and Tamhane et al. (2010) studied the problem of testing a primary and a secondary endpoint, subject to a gatekeeping constraint, using a group sequential design (GSD) with K=2 looks. In this article, we greatly extend the previous results to multiple (K>2) looks. If the familywise error rate (FWER) is to be controlled at a preassigned α level then it is clear that the primary boundary must be of level α. We show under what conditions one α-level primary boundary is uniformly more powerful than another. Based on this result, we recommend the choice of the O'Brien and Fleming (1979) boundary over the Pocock (1977) boundary for the primary endpoint. For the secondary endpoint the choice of the boundary is more complicated since under certain conditions the secondary boundary can be refined to have a nominal level α'>α, while still controlling the FWER at level α, thus boosting the secondary power. We carry out secondary power comparisons via simulation between different choices of primary-secondary boundary combinations. The methodology is applied to the data from the RALES study (Pitt et al., 1999; Wittes et al., 2001). An R library package gsrsb to implement the proposed methodology is made available on CRAN. © 2017, The International Biometric Society.

Composite poly(methyl methacrylate-methacrylic acid-2-hydroxyethyl methacrylate) latex for immunoassay. The case of plasminogen.

PubMed

Miksa, B; Wilczynska, M; Cierniewski, C; Basinska, T; Slomkowski, S

1995-01-01

Poly(methyl methacrylate-methacrylic acid-2-hydroxyethyl methacrylate) latex (ACRYLAT) was synthesized by radical precipitation polymerization. The mass median diameter (MMD) and the geometrical standard deviation (GSD) of the ACRYLAT particles were 138 nm and 1.2, respectively. The concentration of the titrable carboxylic groups in the surface layer of latex particles was equal to 8.41 x 10(-6) mol m-2. Latex was able to bind up to 2.82 x 10(-7) mol of 1-aminopyrene per 1 m2 of the surface of the latex particles due to the ionic interactions between carboxylate anions and ammonium cations of protonated 1-aminopyrene. ACRYLAT was able to immobilize covalently human serum albumin in amounts up to 0.23 mg m-2. Aggregation of ACRYLAT with immobilized HSA, induced with specific antibodies (anti-HSA), was investigated turbidimetrically. The results indicated that in the model turbidimetric immunoassay, ACRYLAT coated with HSA can be used for the detection of anti-HSA in the goat anti-HSA serum diluted from 50 to 7000-fold. Immobilization of rabbit antibodies to plasminogen (anti-Plg) to ACRYLAT via the epsilon-aminocaproic acid linkers provided particles which were used for the development of the turbidimetric immunoassay for plasminogen. In this assay plasminogen could be detected in concentration ranging from 0.75 to 75 micrograms ml-1 in the blood plasma.

Inhalable microparticles containing large payload of anti-tuberculosis drugs.

PubMed

Muttil, Pavan; Kaur, Jatinder; Kumar, Kaushlendra; Yadav, Awadh Bihari; Sharma, Rolee; Misra, Amit

2007-10-01

Microparticles containing large payloads of two anti-tuberculosis (TB) drugs were prepared and evaluated for suitability as a dry powder inhalation targeting alveolar macrophages. A solution containing one part each of isoniazid and rifabutin, plus two parts poly(lactic acid) (L-PLA) was spray-dried. Drug content and in vitro release were assayed by HPLC, and DSC was used to elucidate release behaviour. Particle size was measured by laser scattering and aerosol characteristics by cascade impaction using a Lovelace impactor. Microparticles were administered to mice using an in-house inhalation apparatus or by intra-tracheal instillation. Drugs in solution were administered orally and by intra-cardiac injection. Flow cytometry and HPLC were used to investigate the specificity and magnitude of targeting macrophages. Microparticles having drug content approximately 50% (w/w), particle size approximately 5 microm and satisfactory aerosol characteristics (median mass aerodynamic diameter, MMAD=3.57 microm; geometric standard deviation, GSD=1.41 microm; fine particle fraction, FPF(<4.6 microm)=78.91+/-8.4%) were obtained in yields of >60%. About 70% of the payload was released in vitro in 10 days. Microparticles targeted macrophages and not epithelial cells on inhalation. Drug concentrations in macrophages were approximately 20 times higher when microparticles were inhaled rather than drug solutions administered. Microparticles were thus deemed suitable for enhanced targeted drug delivery to lung macrophages.

Communication Management Guidelines for Software Organizations in Pakistan with clients from Afghanistan

NASA Astrophysics Data System (ADS)

Arif Shah, Muhammad; Hashim, Rathiah; Shah, Adil Ali; Farooq Khattak, Umar

2016-11-01

Developing software through Global Software Development (GSD) became very common now days in the software industry. Pakistan is one of the countries where projects are taken and designed from different countries including Afghanistan. The purpose of this paper is to identify and provide an analysis on several communication barriers that can have a negative impact on the project and to provide management guidelines for medium size software organizations working in Pakistan with clients from Afghanistan and to overcome these communication barriers and challenges organizations face when coordinating with client. Initially we performed a literature review to identify different communication barriers and to check if there are any standardized communications management guidelines for medium size software houses provided in the past. The second stage of the research paper involves guidelines with vendor's perspective that include interviews and focus group discussions with different stakeholders and employees of software houses with clients from Afghanistan. Based on those interviews and discussions we established communication management guidelines in order to overcome the communication problems and barriers working with clients from Afghanistan. As a result of the literature review, we have identified that barriers such as cultural barriers and language barrier were one of the main reasons behind the project failure and suggested that software organizations working in Pakistan should follow certain defined communication guidelines in order to overcome communication barriers that affect the project directly.

Quickbird Satellite in-orbit Modulation Transfer Function (MTF) Measurement Using Edge, Pulse and Impulse Methods for Summer 2003

NASA Technical Reports Server (NTRS)

Helder, Dennis; Choi, Taeyoung; Rangaswamy, Manjunath

2005-01-01

The spatial characteristics of an imaging system cannot be expressed by a single number or simple statement. However, the Modulation Transfer Function (MTF) is one approach to measure the spatial quality of an imaging system. Basically, MTF is the normalized spatial frequency response of an imaging system. The frequency response of the system can be evaluated by applying an impulse input. The resulting impulse response is termed the Point Spread function (PSF). This function is a measure of the amount of blurring present in the imaging system and is itself a useful measure of spatial quality. An underlying assumption is that the imaging system is linear and shift-independent. The Fourier transform of the PSF is called the Optical Transfer Function (OTF) and the normalized magnitude of the OTF is the MTF. In addition to using an impulse input, a knife-edge in technique has also been used in this project. The sharp edge exercises an imaging system at all spatial frequencies. The profile of an edge response from an imaging system is called an Edge Spread Function (ESF). Differentiation of the ESF results in a one-dimensional version of the Point Spread Function (PSF). Finally, MTF can be calculated through use of Fourier transform of the PSF as stated previously. Every image includes noise in some degree which makes MTF of PSF estimation more difficult. To avoid the noise effects, many MTF estimation approaches use smooth numerical models. Historically, Gaussian models and Fermi functions were applied to reduce the random noise in the output profiles. The pulse-input method was used to measure the MTF of the Landsat Thematic Mapper (TM) using 8th order even functions over the San Mateo Bridge in San Francisco, California. Because the bridge width was smaller than the 30-meter ground sample distance (GSD) of the TM, the Nyquist frequency was located before the first zero-crossing point of the sinc function from the Fourier transformation of the bridge pulse. To avoid the zero-crossing points in the frequency domain from a pulse, the pulse width should be less than the width of two pixels (or 2 GSD's), but the short extent of the pulse results in a poor signal-to-noise ratio. Similarly, for a high-resolution satellite imaging system such as Quickbird, the input pulse width was critical because of the zero crossing points and noise present in the background area. It is important, therefore, that the width of the input pulse be appropriately sized. Finally, the MTF was calculated by taking ratio between Fourier transform of output and Fourier transform of input. Regardless of whether the edge, pulse and impulse target method is used, the orientation of the targets is critical in order to obtain uniformly spaced sub-pixel data points. When the orientation is incorrect, sample data points tend to be located in clusters that result in poor reconstruction of the edge or pulse profiles. Thus, a compromise orientation must be selected so that all spectral bands can be accommodated. This report continues by outlining the objectives in Section 2, procedures followed in Section 3, descriptions of the field campaigns in Section 4, results in Section 5, and a brief summary in Section 6.

Comparison of Open Source Compression Algorithms on Vhr Remote Sensing Images for Efficient Storage Hierarchy

NASA Astrophysics Data System (ADS)

Akoguz, A.; Bozkurt, S.; Gozutok, A. A.; Alp, G.; Turan, E. G.; Bogaz, M.; Kent, S.

2016-06-01

High resolution level in satellite imagery came with its fundamental problem as big amount of telemetry data which is to be stored after the downlink operation. Moreover, later the post-processing and image enhancement steps after the image is acquired, the file sizes increase even more and then it gets a lot harder to store and consume much more time to transmit the data from one source to another; hence, it should be taken into account that to save even more space with file compression of the raw and various levels of processed data is a necessity for archiving stations to save more space. Lossless data compression algorithms that will be examined in this study aim to provide compression without any loss of data holding spectral information. Within this objective, well-known open source programs supporting related compression algorithms have been implemented on processed GeoTIFF images of Airbus Defence & Spaces SPOT 6 & 7 satellites having 1.5 m. of GSD, which were acquired and stored by ITU Center for Satellite Communications and Remote Sensing (ITU CSCRS), with the algorithms Lempel-Ziv-Welch (LZW), Lempel-Ziv-Markov chain Algorithm (LZMA & LZMA2), Lempel-Ziv-Oberhumer (LZO), Deflate & Deflate 64, Prediction by Partial Matching (PPMd or PPM2), Burrows-Wheeler Transform (BWT) in order to observe compression performances of these algorithms over sample datasets in terms of how much of the image data can be compressed by ensuring lossless compression.

Formulation, Characterization and Pulmonary Deposition of Nebulized Celecoxib Encapsulated Nanostructured Lipid Carriers

PubMed Central

Patlolla, Ram R.; Chougule, Mahavir; Patel, Apurva R.; Jackson, Tanise; Tata, Prasad NV; Singh, Mandip

2010-01-01

The aim of the current study was to encapsulate celecoxib (Cxb) in the Nanostructured Lipid Carrier (Cxb-NLC) nanoparticles and evaluate the lung disposition of nanoparticles following nebulization in Balb/c mice. Cxb-NLC nanoparticles were prepared with Cxb, Compritol, Miglyol and sodium taurocholate using high-pressure homogenization. Cxb-NLC nanoparticles were characterized for physical and aerosol properties. In-vitro cytotoxicity studies were performed with A549 cells. The lung deposition and pharmacokinetic parameters of Cxb-NLC and Cxb solution (Cxb-Soln) formulations were determined using Inexpose™ system and Pari LC star jet nebulizer. The particle size and entrapment efficiency of Cxb-NLC formulation were 217 ± 20 nm and > 90%, respectively. The Cxb-NLC released the drug in controlled fashion, and in vitro aersolization of Cxb-NLC formulation showed FPF of 75.6 ± 4.6 %, MMAD of 1.6 ±0.13 μm and GSD of 1.2 ± 0.21. Cxb-NLC showed dose and time dependent cytotoxicity against A549 cells. Nebulization of Cxb-NLC demonstrated 4 fold higher AUCt/D in lung tissues compared to Cxb-Soln. The systemic clearance of Cxb-NLC was slower (0.93 L/h) compared to Cxb-Soln (20.03 L/h). Cxb encapsulated NLC were found to be stable and aerodynamic properties were within the respirable limits. Aerosolization of Cxb-NLC improved the Cxb pulmonary bioavailability compared to solution formulation which will potentially lead to better patient compliance with minimal dosing intervals. PMID:20153385

High spatial resolution shortwave infrared imaging technology based on time delay and digital accumulation method

NASA Astrophysics Data System (ADS)

Jia, Jianxin; Wang, Yueming; Zhuang, Xiaoqiong; Yao, Yi; Wang, Shengwei; Zhao, Ding; Shu, Rong; Wang, Jianyu

2017-03-01

Shortwave infrared (SWIR) imaging technology attracts more and more attention by its fascinating ability of penetrating haze and smoke. For application of spaceborne remote sensing, spatial resolution of SWIR is lower compared with that of visible light (VIS) wavelength. It is difficult to balance between the spatial resolution and signal to noise ratio (SNR). Some conventional methods, such as enlarging aperture of telescope, image motion compensation, and analog time delay and integration (TDI) technology are used to gain SNR. These techniques bring in higher cost of satellite, complexity of system or other negative factors. In this paper, time delay and digital accumulation (TDDA) method is proposed to achieve higher spatial resolution. The method can enhance the SNR and non-uniformity of system theoretically. A prototype of SWIR imager consists of opto-mechanical, 1024 × 128 InGaAs detector, and electronics is designed and integrated to prove TDDA method. Both of measurements and experimental results indicate TDDA method can promote SNR of system approximated of the square root of accumulative stage. The results exhibit that non-uniformity of system is also improved by this approach to some extent. The experiment results are corresponded with the theoretical analysis. Based on the experiments results, it is proved firstly that the goal of 1 m ground sample distance (GSD) in orbit of 500 km is feasible with the TDDA stage of 30 for SWIR waveband (0.9-1.7 μm).

Photogrammetric mapping using unmanned aerial vehicle

NASA Astrophysics Data System (ADS)

Graça, N.; Mitishita, E.; Gonçalves, J.

2014-11-01

Nowadays Unmanned Aerial Vehicle (UAV) technology has attracted attention for aerial photogrammetric mapping. The low cost and the feasibility to automatic flight along commanded waypoints can be considered as the main advantages of this technology in photogrammetric applications. Using GNSS/INS technologies the images are taken at the planned position of the exposure station and the exterior orientation parameters (position Xo, Yo, Zo and attitude ω, φ, χ) of images can be direct determined. However, common UAVs (off-the-shelf) do not replace the traditional aircraft platform. Overall, the main shortcomings are related to: difficulties to obtain the authorization to perform the flight in urban and rural areas, platform stability, safety flight, stability of the image block configuration, high number of the images and inaccuracies of the direct determination of the exterior orientation parameters of the images. In this paper are shown the obtained results from the project photogrammetric mapping using aerial images from the SIMEPAR UAV system. The PIPER J3 UAV Hydro aircraft was used. It has a micro pilot MP2128g. The system is fully integrated with 3-axis gyros/accelerometers, GPS, pressure altimeter, pressure airspeed sensors. A Sony Cyber-shot DSC-W300 was calibrated and used to get the image block. The flight height was close to 400 m, resulting GSD near to 0.10 m. The state of the art of the used technology, methodologies and the obtained results are shown and discussed. Finally advantages/shortcomings found in the study and main conclusions are presented

The analysis on the relation between the compression method and the performance enhancement of MSC (Multi-Spectral Camera) image data

NASA Astrophysics Data System (ADS)

Yong, Sang-Soon; Ra, Sung-Woong

2007-10-01

Multi-Spectral Camera(MSC) is a main payload on the KOMPSAT-2 satellite to perform the earth remote sensing. The MSC instrument has one(1) channel for panchromatic imaging and four(4) channel for multi-spectral imaging covering the spectral range from 450nm to 900nm using TDI CCD Focal Plane Array (FPA). The instrument images the earth using a push-broom motion with a swath width of 15 km and a ground sample distance (GSD) of 1 m over the entire field of view (FOV) at altitude 685 Km. The instrument is designed to have an on-orbit operation duty cycle of 20% over the mission lifetime of 3 years with the functions of programmable gain/ offset and on-board image data compression/ storage. The compression method on KOMPSAT-2 MSC was selected and used to match EOS input rate and PDTS output data rate on MSC image data chain. At once the MSC performance was carefully handled to minimize any degradation so that it was analyzed and restored in KGS(KOMPSAT Ground Station) during LEOP and Cal./Val.(Calibration and Validation) phase. In this paper, on-orbit image data chain in MSC and image data processing on KGS including general MSC description is briefly described. The influences on image performance between on-board compression algorithms and between performance restoration methods in ground station are analyzed, and the relation between both methods is to be analyzed and discussed.

The Estimation of Precisions in the Planning of Uas Photogrammetric Surveys

NASA Astrophysics Data System (ADS)

Passoni, D.; Federici, B.; Ferrando, I.; Gagliolo, S.; Sguerso, D.

2018-05-01

The Unmanned Aerial System (UAS) is widely used in the photogrammetric surveys both of structures and of small areas. Geomatics focuses the attention on the metric quality of the final products of the survey, creating several 3D modelling applications from UAS images. As widely known, the quality of results derives from the quality of images acquisition phase, which needs an a priori estimation of the expected precisions. The planning phase is typically managed using dedicated tools, adapted from the traditional aerial-photogrammetric flight plan. But UAS flight has features completely different from the traditional one. Hence, the use of UAS for photogrammetric applications today requires a growth in knowledge in planning. The basic idea of this research is to provide a drone photogrammetric flight planning tools considering the required metric precisions, given a priori the classical parameters of a photogrammetric planning: flight altitude, overlaps and geometric parameters of the camera. The created "office suite" allows a realistic planning of a photogrammetric survey, starting from an approximate knowledge of the Digital Surface Model (DSM), and the effective attitude parameters, changing along the route. The planning products are the overlapping of the images, the Ground Sample Distance (GSD) and the precision on each pixel taking into account the real geometry. The different tested procedures, the obtained results and the solution proposed for the a priori estimates of the precisions in the particular case of UAS surveys are here reported.

Use of Fisheye Parrot Bebop 2 Images for 3d Modelling Using Commercial Photogrammetric Software

NASA Astrophysics Data System (ADS)

Pagliari, D.; Pinto, L.

2018-05-01

Fisheye camera installed on-board mass market UAS are becoming very popular and it is more and more frequent the use of such platforms for photogrammetric purposes. The interest of wide-angles images for 3D modelling is confirmed by the introduction of fisheye models in several commercial software packages. The paper exploits the different mathematical models implemented in the most famous commercial photogrammetric software packages, highlighting the different processing pipelines and analysing the achievable results in terms of checkpoint residuals, as well as the quality of the delivered 3D point clouds. A two-step approach based on the creation of undistorted images has been tested too. An experimental test has been carried out using a Parrot Bebop 2 UAS by performing a flight over an historical complex located near Piacenza (Northern Italy), which is characterized by the simultaneous presence of horizontal, vertical and oblique surfaces. Different flight configurations have been tested to evaluate the potentiality and possible drawbacks of the previously mentioned UAS platform. Results confirmed that the fisheye images acquired with the Parrot Bebop 2 are suitable for 3D modelling, ensuring accuracies of the photogrammetric blocks of the order of the GSD (about 0.05 m normal to the optic axis in case of a flight height equal to 35 m). The generated point clouds have been compared to a reference scan, acquired by means of a MS60 MultiStation, resulting in differences below 0.05 in all directions.

Quantifying Industrial Methane Emissions from Space with the GHGSat-D Satellite

NASA Astrophysics Data System (ADS)

Germain, S.; Durak, B.; Gains, D.; Jervis, D.; McKeever, J.; Sloan, J. J.

2017-12-01

In June 2016, GHGSat, Inc. launched GHGSat-D, or "Claire", the world's first satellite capable of measuring greenhouse gas emissions from targeted industrial facilities around the world. The high-level objective of this mission is to demonstrate that a single measurement approach can quantify methane emission rates from selected industrial sources with greater precision, higher frequency, and lower cost than ground-based alternatives, across a wide range of industries. Providing industrial operators and regulators with frequent, cost-effective emission measurements can help identify super-emitters and monitor the progress of mitigation efforts. The GHGSat measurement platform is a 15 kg satellite that measures methane column densities using a novel wide-angle imaging Fabry-Perot spectrometer tuned to the 1600-1700 nm SWIR band. During each measurement sequence, a series of closely overlapping 2D images are taken so that each ground location samples a portion of the SWIR band with 0.1 nm spectral resolution. The data processing algorithm is able to co-register each image and, by comparison with a detailed forward model, perform a retrieval on each of the <50 m GSD over the entire 12 x 12 km2 field of view. Methane emission rates are then estimated using a dispersion model coupled with locally measured wind fields. We will present the economic rationale for satellite-based sensing of methane from industrial sources, introduce the GHGSat measurement concept, report on recent measurement results obtained by Claire, and describe performance upgrades planned for future missions.

The EarthCARE multi spectral imager thermal infrared optical unit

NASA Astrophysics Data System (ADS)

Chang, M. P. J. L.; Woods, D.; Baister, Guy; Lobb, Dan; Wood, Trevor

2017-11-01

The EarthCARE satellite mission objective is the observation of clouds and aerosols from low Earth orbit. The key spatial context providing instrument within the payload suite of 4 instruments is the Multi-Spectral Imager (MSI), previously described in [1]. The MSI is intended to provide information on the horizontal variability of the atmospheric conditions and to identify e.g. cloud type, textures, and temperature. It will form Earth images at 500m ground sample distance (GSD) over a swath width of 150km; it will image Earth in 7 spectral bands: one visible, one near-IR, two short-wave IR and three thermal IR. The instrument will be comprised of two key parts: • a visible-NIR-SWIR (VNS) optical unit radiometrically calibrated using a sun illuminated quasivolume diffuser and shutter system • a thermal IR (TIR) optical unit radiometrically calibrated using cold space and an internal black-body. This paper, being the first of a sequence of two, will provide an overview of the MSI and enter into more detail the critical performance parameters and detailed design the MSI TIR optical design. The TIR concept is to provide pushbroom imaging of its 3 bands through spectral separation from a common aperture. The result is an efficient, well controlled optical design without the need for multiple focal plane arrays. The designed focal plane houses an area array detector and will meet a challenging set of requirements, including radiometric resolution, accuracy, distortion and MTF.

Potential contributions of heat shock proteins to temperature-dependent sex determination in the American alligator.

PubMed

Kohno, S; Katsu, Y; Urushitani, H; Ohta, Y; Iguchi, T; Guillette, L J

2010-01-01

Sex determination in the American alligator depends on the incubation temperature experienced during a thermo-sensitive period (TSP), although sex determination can be 'reversed' by embryonic exposure to an estrogenic compound. Thus, temperature and estrogenic signals play essential roles during temperature-dependent sex determination (TSD). The genetic basis for TSD is poorly understood, although previous studies observed that many of the genes associated with genetic sex determination (GSD) are expressed in species with TSD. Heat shock proteins (HSPs), good candidates because of their temperature-sensitive expression, have not been examined in regard to TSD but HSPs have the ability to modify steroid receptor function. A number of HSP cDNAs (HSP27, DNAJ, HSP40, HSP47, HSP60, HSP70A, HSP70B, HSP70C, HSP75, HSP90alpha, HSP90beta, and HSP108) as well as cold-inducible RNA binding protein (CIRBP) and HSP-binding protein (HSPBP) were cloned, and expression of their mRNA in the gonadal-adrenal-mesonephros complex (GAM) was investigated. Embryonic and neonatal GAMs exhibited mRNA for all of the HSPs examined during and after the TSP. One-month-old GAMs were separated into 3 portions (gonad, adrenal gland, and mesonephros), and sexual dimorphism in the mRNA expression of gonadal HSP27 (male > female), gonadal HSP70A (male < female), and adrenal HSP90 alpha (male > female) was observed. These findings provide new insights on TSD and suggest that further studies examining the role of HSPs during gonadal development are needed. (c) 2009 S. Karger AG, Basel.

Beryllium contamination inside vehicles of machine shop workers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sanderson, W.T.; Henneberger, P.K.; Martyny, J.

1999-04-01

Inhalation of beryllium particles causes a chronic, debilitating lung disease--chronic beryllium disease (CBD)--in immunologically sensitized workers. Evidence that very low concentrations of beryllium may initiate this chronic disease is provided by incidences of the illness in family members exposed to beryllium dust from workers` clothes and residents in neighborhoods surrounding beryllium refineries. This article describes the results of a cross-sectional survey to evaluate potential take-home beryllium exposures by measuring surface concentrations on the hands and in vehicles of workers at a precision machine shop where cases of CBD had recently been diagnosed. Many workers did not change out of theirmore » work clothes and shoes at the end of their shift, increasing the risk of taking beryllium home to their families. Wipe samples collected from workers` hands and vehicle surfaces were analyzed for beryllium content by inductively coupled argon plasma-atomic emission spectroscopy (ICP-AES). The results ranged widely, from nondetectable to 40 {micro}g/ft{sup 2} on workers` hands and up to 714 {micro}g/fg{sup 2} inside their vehicles, demonstrating that many workers carried residual beryllium on their hands and contaminated the inside of their vehicles when leaving work. The highest beryllium concentrations inside the workers` vehicles were found on the drivers` floor (GM = 19 {micro}g/ft{sup 2}, GSD = 4.9), indicating that workers were carrying beryllium on their shoes into their vehicles. A safe level of beryllium contamination on surfaces is not known, but it is prudent to reduce the potential for workers to carry beryllium away from the work site.« less

Factors affecting gestation duration in the bitch.

PubMed

Eilts, Bruce E; Davidson, Autumn P; Hosgood, Giselle; Paccamonti, Dale L; Baker, David G

2005-07-15

A retrospective analysis was performed to determine the effects of age, breed, parity, and litter size on the duration of gestation in the bitch. Bitches at two locations were monitored from breeding to whelping. A total of 764 litters whelped from 308 bitches (36 large hounds, 34 Golden Retrievers, 23 German Shepherd Dogs (GSD), and 215 Labrador Retrievers). By breed, the number of whelpings was 152, 72, 58, and 482 for the hounds, Golden Retrievers, German Shepherd Dogs, and Labrador Retrievers, respectively. Whelping was predicted to be 57 d from the first day of cytologic diestrus in the hounds or 65 d from the initial progesterone rise in the other breeds. The average gestation duration (calculated as 8 d prior to Day 1 of cytologic diestrus in hounds or measured from the initial progesterone rise in other breeds) by breed (days +/- S.D.) was 66.0 +/- 2.8, 64.7 +/- 1.5, 63.6 +/- 2.1, and 62.9 +/- 1.3 for the hounds, Golden Retrievers, German Shepherd Dogs, and Labrador Retrievers, respectively. The relationship of age, breed, parity, and litter size with the difference in gestation duration was evaluated using log linear modeling. Age or parity had no effect on gestation duration. Compared to Labrador Retrievers, the German Shepherd Dogs, Golden Retrievers and hounds were more likely to have a longer gestation duration; three, four and nearly eight times as likely, respectively. Bitches whelping four or fewer pups were significantly more likely to have a longer gestation duration than those whelping five or more pups; the prolongation averaging 1 d.

Effect of drug load and plate coating on the particle size distribution of a commercial albuterol metered dose inhaler (MDI) determined using the Andersen and Marple-Miller cascade impactors.

PubMed

Nasr, M M; Ross, D L; Miller, N C

1997-10-01

The purpose of this study is to investigate the effect of drug load, the coating of impactor stages, and the design of cascade impactors on albuterol MDIs particle size distribution measurements. The results of the investigation will be used to explain the "loading effect" recently reported. Particle size distribution parameters of a commercial albuterol MDI were measured using both Andersen (AI) and Marple-Miller (MMI) Cascade Impactors, where plates were either left uncoated or coated with silicone or glycerin. A previously validated HPLC-EC method was used for the assay of albuterol collected by the impactor and in single spray content determinations. Coating impactor collection plates had an impact on measured MMAD and GSD values for single puff measurements but very little or no effect for the multi puff measurements. Due to particle bounce, the percent of albuterol fine particles deposited in the filter and impactor finer stages (< 1.10 microns in AI and < 1.25 microns in MMI) in uncoated single puff experiments was much higher in comparison to either coated single puff or multi-puff (coated and uncoated) measurements. Evaluation of drug load and plate coating are necessary to determine whether observed particle size distributions are representative of the generated aerosol or are the result of particle bounce and reentrainment. In order to minimize particle bounce, especially for single puff determinations, it may be useful to apply a thin layer of a sticky coating agent to the surfaces of impactor plates.

Assessment Study of Using Online (CSRS) GPS-PPP Service for Mapping Applications in Egypt

NASA Astrophysics Data System (ADS)

Abd-Elazeem, Mohamed; Farah, Ashraf; Farrag, Farrag

2011-09-01

Many applications in navigation, land surveying, land title definitions and mapping have been made simpler and more precise due to accessibility of Global Positioning System (GPS) data, and thus the demand for using advanced GPS techniques in surveying applications has become essential. The differential technique was the only source of accurate positioning for many years, and remained in use despite of its cost. The precise point positioning (PPP) technique is a viable alternative to the differential positioning method in which a user with a single receiver can attain positioning accuracy at the centimeter or decimeter scale. In recent years, many organizations introduced online (GPS-PPP) processing services capable of determining accurate geocentric positions using GPS observations. These services provide the user with receiver coordinates in free and unlimited access formats via the internet. This paper investigates the accuracy of the Canadian Spatial Reference System (CSRS) Precise Point Positioning (PPP) (CSRS-PPP) service supervised by the Geodetic Survey Division (GSD), Canada. Single frequency static GPS observations have been collected at three points covering time spans of 60, 90 and 120 minutes. These three observed sites form baselines of 1.6, 7, and 10 km, respectively. In order to assess the CSRS-PPP accuracy, the discrepancies between the CSRS-PPP estimates and the regular differential GPS solutions were computed. The obtained results illustrate that the PPP produces a horizontal error at the scale of a few decimeters; this is accurate enough to serve many mapping applications in developing countries with a savings in both cost and experienced labor.

L-pyroglutamic acid inhibits energy production and lipid synthesis in cerebral cortex of young rats in vitro.

PubMed

Silva, A R; Silva, C G; Ruschel, C; Helegda, C; Wyse, A T; Wannmacher, C M; Wajner, M; Dutra-Filho, C S

2001-12-01

In the present study we investigated the effects of L-pyroglutamic acid (PGA), which predominantly accumulates in the inherited metabolic diseases glutathione synthetase deficiency (GSD) and gamma-glutamylcysteine synthetase deficiency (GCSD), on some in vitro parameters of energy metabolism and lipid biosynthesis. We evaluated the rates of CO2 production and lipid synthesis from [U-14C]acetate, as well as ATP levels and the activities of creatine kinase and of the respiratory chain complexes I-IV in cerebral cortex of young rats in the presence of PGA at final concentrations ranging from 0.5 to 3 mM. PGA significantly reduced brain CO2 production by 50% at the concentrations of 0.5 to 3 mM, lipid biosynthesis by 20% at concentrations of 0.5 to 3 mM and ATP levels by 52% at the concentration of 3 mM. Regarding the enzyme activities, PGA significantly decreased NADH:cytochrome c oxireductase (complex I plus CoQ plus complex III) by 40% at concentrations of 0.5-3.0 mM and cytochrome c oxidase activity by 22-30% at the concentration of 3.0 mM, without affecting the activities of succinate dehydrogenase, succinate:DCPIP oxireductase (complex II), succinate:cytochrome c oxireductase (complex II plus CoQ plus complex III) or creatine kinase. The results strongly indicate that PGA impairs brain energy production. If these effects also occur in humans, it is possible that they may contribute to the neuropathology of patients affected by these diseases.

Analysis of the Radiometric Response of Orange Tree Crown in Hyperspectral Uav Images

NASA Astrophysics Data System (ADS)

Imai, N. N.; Moriya, E. A. S.; Honkavaara, E.; Miyoshi, G. T.; de Moraes, M. V. A.; Tommaselli, A. M. G.; Näsi, R.

2017-10-01

High spatial resolution remote sensing images acquired by drones are highly relevant data source in many applications. However, strong variations of radiometric values are difficult to correct in hyperspectral images. Honkavaara et al. (2013) presented a radiometric block adjustment method in which hyperspectral images taken from remotely piloted aerial systems - RPAS were processed both geometrically and radiometrically to produce a georeferenced mosaic in which the standard Reflectance Factor for the nadir is represented. The plants crowns in permanent cultivation show complex variations since the density of shadows and the irradiance of the surface vary due to the geometry of illumination and the geometry of the arrangement of branches and leaves. An evaluation of the radiometric quality of the mosaic of an orange plantation produced using images captured by a hyperspectral imager based on a tunable Fabry-Pérot interferometer and applying the radiometric block adjustment method, was performed. A high-resolution UAV based hyperspectral survey was carried out in an orange-producing farm located in Santa Cruz do Rio Pardo, state of São Paulo, Brazil. A set of 25 narrow spectral bands with 2.5 cm of GSD images were acquired. Trend analysis was applied to the values of a sample of transects extracted from plants appearing in the mosaic. The results of these trend analysis on the pixels distributed along transects on orange tree crown showed the reflectance factor presented a slightly trend, but the coefficients of the polynomials are very small, so the quality of mosaic is good enough for many applications.

Melt dispersion granules: formulation and evaluation to improve oral delivery of poorly soluble drugs - a case study with valsartan.

PubMed

Chella, Naveen; Tadikonda, Ramarao

2015-06-01

Solid dispersion (SD) technique is a promising strategy to improve the solubility and dissolution of BCS class II drugs. However, only few products are marketed till today based on SD technology due to poor flow properties and stability. The present work was intended to solve these problems by using combination approach, melt dispersion and surface adsorption technologies. The main aim of the present work is to improve the absorption in the stomach (at lower pH) where the absorption window exists for the drug by improving the dissolution, resulting in the enhancement of oral bioavailability of poorly soluble, weakly acidic drug with pH dependant solubility, i.e. valsartan. Melt dispersion granules were prepared in different ratios using different carriers (Gelucire 50/13, PEG 8000 and Pluronic F-68) and lactose as an adsorbent. Similarly, physical mixtures were also prepared at corresponding ratios. The prepared dispersion granules and physical mixtures were characterized by FTIR, DSC and in vitro dissolution studies. DSC studies revealed reduction in the crystallinity with a possibility of presence of amorphous character of drug in the dispersion granules. From dissolution studies, valsartan Gelucire dispersion (GSD4; 1:4 ratio) showed complete drug release in 30 min against the plain drug which showed only 11.31% of drug release in 30 min. Pharmacokinetic studies of optimized formulation in male Wistar rats showed 2.65-fold higher bioavailability and 1.47-fold higher Cmax compared to pure drug. The melt dispersion technology has the potential to improve dissolution and the bioavailability of BCS class II drugs.

Fusion of pixel and object-based features for weed mapping using unmanned aerial vehicle imagery

NASA Astrophysics Data System (ADS)

Gao, Junfeng; Liao, Wenzhi; Nuyttens, David; Lootens, Peter; Vangeyte, Jürgen; Pižurica, Aleksandra; He, Yong; Pieters, Jan G.

2018-05-01

The developments in the use of unmanned aerial vehicles (UAVs) and advanced imaging sensors provide new opportunities for ultra-high resolution (e.g., less than a 10 cm ground sampling distance (GSD)) crop field monitoring and mapping in precision agriculture applications. In this study, we developed a strategy for inter- and intra-row weed detection in early season maize fields from aerial visual imagery. More specifically, the Hough transform algorithm (HT) was applied to the orthomosaicked images for inter-row weed detection. A semi-automatic Object-Based Image Analysis (OBIA) procedure was developed with Random Forests (RF) combined with feature selection techniques to classify soil, weeds and maize. Furthermore, the two binary weed masks generated from HT and OBIA were fused for accurate binary weed image. The developed RF classifier was evaluated by 5-fold cross validation, and it obtained an overall accuracy of 0.945, and Kappa value of 0.912. Finally, the relationship of detected weeds and their ground truth densities was quantified by a fitted linear model with a coefficient of determination of 0.895 and a root mean square error of 0.026. Besides, the importance of input features was evaluated, and it was found that the ratio of vegetation length and width was the most significant feature for the classification model. Overall, our approach can yield a satisfactory weed map, and we expect that the obtained accurate and timely weed map from UAV imagery will be applicable to realize site-specific weed management (SSWM) in early season crop fields for reducing spraying non-selective herbicides and costs.

Novel method for detection of glycogen in cells

PubMed Central

Segvich, Dyann M; DePaoli-Roach, Anna A; Roach, Peter J

2017-01-01

Abstract Glycogen, a branched polymer of glucose, functions as an energy reserve in many living organisms. Abnormalities in glycogen metabolism, usually excessive accumulation, can be caused genetically, most often through mutation of the enzymes directly involved in synthesis and degradation of the polymer leading to a variety of glycogen storage diseases (GSDs). Microscopic visualization of glycogen deposits in cells and tissues is important for the study of normal glycogen metabolism as well as diagnosis of GSDs. Here, we describe a method for the detection of glycogen using a renewable, recombinant protein which contains the carbohydrate-binding module (CBM) from starch-binding domain containing protein 1 (Stbd1). We generated a fusion protein containing glutathione S-transferase, a cMyc eptitope and the Stbd1CBM (GYSC) for use as a glycogen-binding probe, which can be detected with secondary antibodies against glutathione S-transferase or cMyc. By enzyme-linked immunosorbent assay, we demonstrate that GYSC binds glycogen and two other polymers of glucose, amylopectin and amylose. Immunofluorescence staining of cultured cells indicate a GYSC-specific signal that is co-localized with signals obtained with anti-glycogen or anti-glycogen synthase antibodies. GYSC-positive staining inside of lysosomes is observed in individual muscle fibers isolated from mice deficient in lysosomal enzyme acid alpha-glucosidase, a well-characterized model of GSD II (Pompe disease). Co-localized GYSC and glycogen signals are also found in muscle fibers isolated from mice deficient in malin, a model for Lafora disease. These data indicate that GYSC is a novel probe that can be used to study glycogen metabolism under normal and pathological conditions. PMID:28077463

Development of detailed design concepts for the EarthCARE multi-spectral imager

NASA Astrophysics Data System (ADS)

Lobb, Dan; Escadero, Isabel; Chang, Mark; Gode, Sophie

2017-11-01

The EarthCARE mission is dedicated to the study of clouds by observations from a satellite in low Earth orbit. The payload will include major radar and LIDAR instruments, supported by a multi-spectral imager (MSI) and a broadband radiometer. The paper describes development of detailed design concepts for the MSI, and analysis of critical performance parameters. The MSI will form Earth images at 500m ground sample distance (GSD) over a swath width of 150km, from a nominal platform altitude of around 400km. The task of the MSI is to provide spatial context for the single-point measurements made by the radar and LIDAR systems; it will image Earth in 7 spectral bands: one visible, one near-IR, two short-wave IR and three thermal IR. The MSI instrument will be formed in two parts: a visible-NIR-SWIR (VNS) system, radiometrically calibrated using a sunilluminated diffuser, and a thermal IR (TIR) system calibrated using cold space and an internal black-body. The VNS system will perform push-broom imaging, using linear array detectors (silicon and InGaAs) and 4 separate lenses. The TIR system will use a microbolometer array detector in a time delay and integration (TDI) mode. Critical issues discussed for the VNS system include detector selection and detailed optical design trade-offs. The latter are related to the desirability of dichroics to achieve a common aperture, which influences the calibration hardware and lens design. The TIR system's most significant problems relate to control of random noise and bias errors, requiring optimisation of detector operation and calibration procedures.

The FIM-iHYCOM Model in SubX: Evaluation of Subseasonal Errors and Variability

NASA Astrophysics Data System (ADS)

Green, B.; Sun, S.; Benjamin, S.; Grell, G. A.; Bleck, R.

2017-12-01

NOAA/ESRL/GSD has produced both real-time and retrospective forecasts for the Subseasonal Experiment (SubX) using the FIM-iHYCOM model. FIM-iHYCOM couples the atmospheric Flow-following finite volume Icosahedral Model (FIM) to an icosahedral-grid version of the Hybrid Coordinate Ocean Model (HYCOM). This coupled model is unique in terms of its grid structure: in the horizontal, the icosahedral meshes are perfectly matched for FIM and iHYCOM, eliminating the need for a flux interpolator; in the vertical, both models use adaptive arbitrary Lagrangian-Eulerian hybrid coordinates. For SubX, FIM-iHYCOM initializes four time-lagged ensemble members around each Wednesday, which are integrated forward to provide 32-day forecasts. While it has already been shown that this model has similar predictive skill as NOAA's operational CFSv2 in terms of the RMM index, FIM-iHYCOM is still fairly new and thus its overall performance needs to be thoroughly evaluated. To that end, this study examines model errors as a function of forecast lead week (1-4) - i.e., model drift - for key variables including 2-m temperature, precipitation, and SST. Errors are evaluated against two reanalysis products: CFSR, from which FIM-iHYCOM initial conditions are derived, and the quasi-independent ERA-Interim. The week 4 error magnitudes are similar between FIM-iHYCOM and CFSv2, albeit with different spatial distributions. Also, intraseasonal variability as simulated in these two models will be compared with reanalyses. The impact of hindcast frequency (4 times per week, once per week, or once per day) on the model climatology is also examined to determine the implications for systematic error correction in FIM-iHYCOM.

SkySat-1: very high-resolution imagery from a small satellite

NASA Astrophysics Data System (ADS)

Murthy, Kiran; Shearn, Michael; Smiley, Byron D.; Chau, Alexandra H.; Levine, Josh; Robinson, M. Dirk

2014-10-01

This paper presents details of the SkySat-1 mission, which is the first microsatellite-class commercial earth- observation system to generate sub-meter resolution panchromatic imagery, in addition to sub-meter resolution 4-band pan-sharpened imagery. SkySat-1 was built and launched for an order of magnitude lower cost than similarly performing missions. The low-cost design enables the deployment of a large imaging constellation that can provide imagery with both high temporal resolution and high spatial resolution. One key enabler of the SkySat-1 mission was simplifying the spacecraft design and instead relying on ground- based image processing to achieve high-performance at the system level. The imaging instrument consists of a custom-designed high-quality optical telescope and commercially-available high frame rate CMOS image sen- sors. While each individually captured raw image frame shows moderate quality, ground-based image processing algorithms improve the raw data by combining data from multiple frames to boost image signal-to-noise ratio (SNR) and decrease the ground sample distance (GSD) in a process Skybox calls "digital TDI". Careful qual-ity assessment and tuning of the spacecraft, payload, and algorithms was necessary to generate high-quality panchromatic, multispectral, and pan-sharpened imagery. Furthermore, the framing sensor configuration en- abled the first commercial High-Definition full-frame rate panchromatic video to be captured from space, with approximately 1 meter ground sample distance. Details of the SkySat-1 imaging instrument and ground-based image processing system are presented, as well as an overview of the work involved with calibrating and validating the system. Examples of raw and processed imagery are shown, and the raw imagery is compared to pre-launch simulated imagery used to tune the image processing algorithms.

Nano-liposomal dry powder inhaler of tacrolimus: preparation, characterization, and pulmonary pharmacokinetics.

PubMed

Chougule, Mahavir; Padhi, Bijay; Misra, Ambikanandan

2007-01-01

The studies were undertaken to evaluate feasibility of pulmonary delivery of liposomaly encapsulated tacrolimus dry powder inhaler for prolonged drug retention in lungs as rescue therapy to prevent refractory rejection of lungs after transplantation. Tacrolimus encapsulated liposomes were prepared by thin film evaporation technique and liposomal dispersion was passed through high pressure homogenizer. Tacrolimus nano-liposomes (NLs) were separated by centrifugation and characterized. NLs were dispersed in phosphate buffer saline (PBS) pH 7.4 containing different additives like lactose, sucrose, and trehalose, and L-leucine as antiadherent. The dispersion was spray dried and spray dried powders were characterized. In vitro and in vivo pulmonary deposition was performed using Andersen Cascade Impactor and intratracheal instillation in rats respectively. NLs were found to have average size of 140 nm, 96% +/- 1.5% drug entrapment, and zeta potential of 1.107 mV. Trehalose based formulation was found to have low density, good flowability, particle size of 9.46 +/- 0.8 microm, maximum fine particle fraction (FPF) of 71.1 +/- 2.5%, mean mass aerodynamic diameter (MMAD) 2.2 +/- 0.1 microm, and geometric standard deviation (GSD) 1.7 +/- 0.2. Developed formulations were found to have in vitro prolonged drug release up to 18 hours, following Higuchi's Controlled Release model. In vivo studies revealed maximal residence of tacrolimus within lungs of 24 hours, suggesting slow clearance from the lungs. The investigation provides a practical approach for direct delivery of tacrolimus encapsulated in NLs for controlled and prolonged retention at the site of action. It may play a promising role as rescue therapy in reducing the risk of acute rejection and chronic rejection.

Nano-liposomal dry powder inhaler of tacrolimus: Preparation, characterization, and pulmonary pharmacokinetics

PubMed Central

Chougule, Mahavir; Padhi, Bijay; Misra, Ambikanandan

2007-01-01

The studies were undertaken to evaluate feasibility of pulmonary delivery of liposomaly encapsulated tacrolimus dry powder inhaler for prolonged drug retention in lungs as rescue therapy to prevent refractory rejection of lungs after transplantation. Tacrolimus encapsulated liposomes were prepared by thin film evaporation technique and liposomal dispersion was passed through high pressure homogenizer. Tacrolimus nano-liposomes (NLs) were separated by centrifugation and characterized. NLs were dispersed in phosphate buffer saline (PBS) pH 7.4 containing different additives like lactose, sucrose, and trehalose, and L-leucine as antiadherent. The dispersion was spray dried and spray dried powders were characterized. In vitro and in vivo pulmonary deposition was performed using Andersen Cascade Impactor and intratracheal instillation in rats respectively. NLs were found to have average size of 140 nm, 96% ± 1.5% drug entrapment, and zeta potential of 1.107 mV. Trehalose based formulation was found to have low density, good flowability, particle size of 9.46 ± 0.8 μm, maximum fine particle fraction (FPF) of 71.1 ± 2.5%, mean mass aerodynamic diameter (MMAD) 2.2 ± 0.1 μm, and geometric standard deviation (GSD) 1.7 ± 0.2. Developed formulations were found to have in vitro prolonged drug release up to 18 hours, following Higuchi’s Controlled Release model. In vivo studies revealed maximal residence of tacrolimus within lungs of 24 hours, suggesting slow clearance from the lungs. The investigation provides a practical approach for direct delivery of tacrolimus encapsulated in NLs for controlled and prolonged retention at the site of action. It may play a promising role as rescue therapy in reducing the risk of acute rejection and chronic rejection. PMID:18203434

Datum Transformation of Spatial Data and Application in Cadastre

NASA Astrophysics Data System (ADS)

Kısa, A.; Erkek, B.; Ekin, L.

2012-07-01

In Turkey, cadastral works have been started with local-based works in 1924 and speeded up after 1950's by using photogrammetry. Different measurement methods, coordinate systems and scales have been used in these works. As a result of primary cadastral activities two main products are generated; cadastral maps and title deeds. After this, cadastral data live on the maps, by cadastral activities carried out by cadastral offices and title deed data live on the registrations by land registration activities carried out by land registration offices. Up to 2005 different references systems such as local (graphic) and ED50 have been used for Cadastral maps production. 2000's Land Registry and Cadastre Information System (TAKBİS) Project has started as a pilot application by Land Registry and Cadastre (TKGM). After completion of pilot project spreading activities started in 2005 and still has been ongoing. On the other hand The government has taken the decision to finish primary cadastral activities within three years. The primary cadastral activities completed at the end of 2008. And also TKGM has completed metadata portal in 2008. At last, cadastral map updating (renovation) started in 2009 by using digital orthophoto with 30 cm GSD. Today people have great expectations in accomplishing digital cadastral services, they need correct, reliable, easy and quick accessible land register and cadastral survey information. Even such request expressed in INPIRE directive by using ISO 191XX data standards. This means we have great hard work for spatial data conversion, datum and data transformation for map and cadastral data harmonization. This paper presents results of investigation of used cadastral maps and used datums of the TKGM and possible transformation methods of datum and some recommendations for future applications.

Direct Georeferencing on Small Unmanned Aerial Platforms for Improved Reliability and Accuracy of Mapping Without the Need for Ground Control Points

NASA Astrophysics Data System (ADS)

Mian, O.; Lutes, J.; Lipa, G.; Hutton, J. J.; Gavelle, E.; Borghini, S.

2015-08-01

This paper presents results from a Direct Mapping Solution (DMS) comprised of an Applanix APX-15 UAV GNSS-Inertial system integrated with a Sony a7R camera to produce highly accurate ortho-rectified imagery without Ground Control Points on a Microdrones md4-1000 platform. A 55 millimeter Nikkor f/1.8 lens was mounted on the Sony a7R and the camera was then focused and calibrated terrestrially using the Applanix camera calibration facility, and then integrated with the APX-15 UAV GNSS-Inertial system using a custom mount specifically designed for UAV applications. In July 2015, Applanix and Avyon carried out a test flight of this system. The goal of the test flight was to assess the performance of DMS APX-15 UAV direct georeferencing system on the md4-1000. The area mapped during the test was a 250 x 300 meter block in a rural setting in Ontario, Canada. Several ground control points are distributed within the test area. The test included 8 North-South lines and 1 cross strip flown at 80 meters AGL, resulting in a ~1 centimeter Ground Sample Distance (GSD). Map products were generated from the test flight using Direct Georeferencing, and then compared for accuracy against the known positions of ground control points in the test area. The GNSS-Inertial data collected by the APX-15 UAV was post-processed in Single Base mode, using a base station located in the project area via POSPac UAV. The base-station's position was precisely determined by processing a 12-hour session using the CSRS-PPP Post Processing service. The ground control points were surveyed in using differential GNSS post-processing techniques with respect to the base-station.

Overview of particulate exposures in the US trucking industry

PubMed Central

Smith, Thomas J.; Davis, Mary E.; Reaser, Paul; Natkin, Jonathan; Hart, Jaime E; Laden, Francine; Heff, Allan; Garshick, Eric

2007-01-01

As part of a large epidemiologic study of lung cancer, 55,000 subjects, we have conducted a nation-wide survey of particulate exposures in the US trucking industry. The goal is to differentiate the risks from various types of particulate exposures, such as traffic emissions and general air pollution. We hypothesize that exposures defined by job and work site characteristics can be linked with subjects using their personal job histories. This report covers exposures at 36 randomly sited large truck freight terminals in the US. Measurements were made of PM2.5, elemental carbon (EC), and organic carbon (OC) upwind of the terminal (background) and in work areas, and by personal samples. Significant differences in exposure intensity, dg/m3, were found for work locations and jobs relative to background levels (GM[GSD]) at terminal sites: PM2.5 9.8[2.34], EC 0.5[3.24], and OC 5.0[1.76]. Using EC as a marker for diesel particles, work locations varied significantly: office 0.3[3.7], dock area 0.7[2.89] and shop area 1.5[3.52]), as did job titles (non-smokers): clerk 0.1[9.98], dockworker 0.8[2.13], and mechanic 2.0[3.82]. Cigarette smoking contributed substantially to personal exposures, approximately doubling PM2.5 and OC, but having less of an effect on EC. Large differences were seen across the terminal sites due to differences in local regional air pollution levels from traffic and other sources. We conclude that it will be possible to estimate current exposures of the cohort using an exposure assignment matrix based on job title, work location, and terminal site. This distribution overlaps substantially with the general public’s exposure to these sources. PMID:16826284

Factors determining the exposure of dairy farmers to thoracic organic dust.

PubMed

Pfister, Hugo; Madec, Laurent; Cann, Pierre Le; Costet, Nathalie; Chouvet, Martine; Jouneau, Stéphane; Vernhet, Laurent

2018-08-01

Bronchial respiratory diseases are more common in dairy farmers than in the general population, perhaps because the repeated inhalation of organic dust contributes to the development of these disorders. However, the factors determining the exposure of farmers to particles that can enter the lower bronchial tract and interact with it, i.e. the thoracic fraction of the inhalable dust, remain to be identified. We therefore measured the exposure of dairy farmers to thoracic organic dust and identified the farm features and tasks that increased exposure. We measured thoracic particles (n = 110) and farm characteristics and occupational tasks in 29 Brittany dairy farms. The mean (GM) (geometric standard deviation, GSD) concentration of thoracic dust in air inhaled by farmers was 0.24 mg/m 3 (2.8) and the concentrations of endotoxins, Gram-positive bacteria and fungi in the thoracic fraction were 128 EU/m 3 (4.0), 960 CFU/m 3 (6.3) and 690 CFU/m 3 (5.4), respectively. Model-based estimates of the association between exposure, farm features and tasks indicated that manual grain and feed handling and mechanical bedding spreading significantly increased exposure to thoracic dust, endotoxins, bacteria and fungi. Exposure to bacteria and fungi was reduced by cowsheds divided into cubicles, whereas using automatic muck scrappers in alleyway and automatic milking tended to increase exposure to bacteria and endotoxins. Finally, exposure to endotoxin and fungi were reduced by warmer farm buildings and well-ventilated buildings having walls with large openings. In conclusions, major occupational tasks and specific farm features determine the exposure of Breton dairy farmers to thoracic organic dust. Copyright © 2018 Elsevier Inc. All rights reserved.

An automated, open-source (NASA Ames Stereo Pipeline) workflow for mass production of high-resolution DEMs from commercial stereo satellite imagery: Application to mountain glacies in the contiguous US

NASA Astrophysics Data System (ADS)

Shean, D. E.; Arendt, A. A.; Whorton, E.; Riedel, J. L.; O'Neel, S.; Fountain, A. G.; Joughin, I. R.

2016-12-01

We adapted the open source NASA Ames Stereo Pipeline (ASP) to generate digital elevation models (DEMs) and orthoimages from very-high-resolution (VHR) commercial imagery of the Earth. These modifications include support for rigorous and rational polynomial coefficient (RPC) sensor models, sensor geometry correction, bundle adjustment, point cloud co-registration, and significant improvements to the ASP code base. We outline an automated processing workflow for 0.5 m GSD DigitalGlobe WorldView-1/2/3 and GeoEye-1 along-track and cross-track stereo image data. Output DEM products are posted at 2, 8, and 32 m with direct geolocation accuracy of <5.0 m CE90/LE90. An automated iterative closest-point (ICP) co-registration tool reduces absolute vertical and horizontal error to <0.5­ m where appropriate ground-control data are available, with observed standard deviation of 0.1-0.5 m for overlapping, co-registered DEMs (n=14,17). While ASP can be used to process individual stereo pairs on a local workstation, the methods presented here were developed for large-scale batch processing in a high-performance computing environment. We have leveraged these resources to produce dense time series and regional mosaics for the Earth's ice sheets. We are now processing and analyzing all available 2008-2016 commercial stereo DEMs over glaciers and perennial snowfields in the contiguous US. We are using these records to study long-term, interannual, and seasonal volume change and glacier mass balance. This analysis will provide a new assessment of regional climate change, and will offer basin-scale analyses of snowpack evolution and snow/ice melt runoff for water resource applications.

Fisheye Photogrammetry: Tests and Methodologies for the Survey of Narrow Spaces

NASA Astrophysics Data System (ADS)

Perfetti, L.; Polari, C.; Fassi, F.

2017-02-01

The research illustrated in this article aimed at identifying a good standard methodology to survey very narrow spaces during 3D investigation of Cultural Heritage. It is an important topic in today's era of BIM modelling applied to Cultural Heritage. Spaces like staircases, corridors and passages are very common in the architectural or archaeological fields, and obtaining a 3D-oriented survey of those areas can be a very complex task when completeness of the model and high precision are requested. Photogrammetry appears to be the most promising solution in terms of versatility and manoeuvrability also considering the quality of the required data. Fisheye lenses were studied and tested in depth because of their significant advantage in the field of view if compared with rectilinear lenses. This advantage alone can be crucial to reduce the total amount of photos and, as a consequence, to obtain manageable data, to simplify the survey phase and to significantly reduce the elaboration time. In order to overcome the main issue that arise when using fisheye lenses, which is the lack of rules that can be employed to design the survey, a general mathematical formulation to precisely estimate the GSD (Ground Sampling Distance) for every optical projection is presented here. A complete survey of a real complex case study was performed in order to test and stress the proposed methodology, and to handle a fisheye-based survey from beginning to end: the photogrammetric survey of the Minguzzi Staircase. It is a complex service spiral-staircase located in the Duomo di Milano with a total height of 25 meters and characterized by a narrow walkable space about 70 centimetres wide.

Concentrations of fine, ultrafine, and black carbon particles in auto-rickshaws in New Delhi, India

NASA Astrophysics Data System (ADS)

Apte, Joshua, S.; Kirchstetter, Thomas W.; Reich, Alexander, H.; Deshpande, Shyam J.; Kaushik, Geetanjali; Chel, Arvind; Marshall, Julian D.; Nazaroff, William W.

2011-08-01

Concentrations of air pollutants from vehicles are elevated along roadways, indicating that human exposure in transportation microenvironments may not be adequately characterized by centrally located monitors. We report results from ˜180 h of real-time measurements of fine particle and black carbon mass concentration (PM 2.5, BC) and ultrafine particle number concentration (PN) inside a common vehicle, the auto-rickshaw, in New Delhi, India. Measured exposure concentrations are much higher in this study (geometric mean for ˜60 trip-averaged concentrations: 190 μg m -3 PM 2.5, 42 μg m -3 BC, 280 × 10 3 particles cm -3; GSD ˜1.3 for all three pollutants) than reported for transportation microenvironments in other megacities. In-vehicle concentrations exceeded simultaneously measured ambient levels by 1.5× for PM 2.5, 3.6× for BC, and 8.4× for PN. Short-duration peak concentrations (averaging time: 10 s), attributable to exhaust plumes of nearby vehicles, were greater than 300 μg m -3 for PM 2.5, 85 μg m -3 for BC, and 650 × 10 3 particles cm -3 for PN. The incremental increase of within-vehicle concentration above ambient levels—which we attribute to in- and near-roadway emission sources—accounted for 30%, 68% and 86% of time-averaged in-vehicle PM 2.5, BC and PN concentrations, respectively. Based on these results, we estimate that one's exposure during a daily commute by auto-rickshaw in Delhi is as least as large as full-day exposures experienced by urban residents of many high-income countries. This study illuminates an environmental health concern that may be common in many populous, low-income cities.

Overview of particulate exposures in the US trucking industry.

PubMed

Smith, Thomas J; Davis, Mary E; Reaser, Paul; Natkin, Jonathan; Hart, Jaime E; Laden, Francine; Heff, Allan; Garshick, Eric

2006-07-01

As part of a large epidemiologic study of lung cancer, 55,000 subjects, we have conducted a nation-wide survey of particulate exposures in the US trucking industry. The goal is to differentiate the risks from various types of particulate exposures, such as traffic emissions and general air pollution. We hypothesize that exposures defined by job and work site characteristics can be linked with subjects using their personal job histories. This report covers exposures at 36 randomly chosen large truck freight terminals in the US. Measurements were made of PM2.5, elemental carbon (EC), and organic carbon (OC) upwind of the terminal (background) and in work areas, and by personal samples. Significant differences in exposure intensity, microg m(-3), were found for work locations and jobs relative to background levels (GM[GSD]) at terminal sites: PM2.5 9.8[2.34], EC 0.5[3.24], and OC 5.0[1.76]. Using EC as a marker for diesel particles, work locations varied significantly: office 0.3[3.7], dock area 0.7[2.89] and shop area 1.5[3.52]), as did job titles (non-smokers): clerk 0.1[9.98], dock worker 0.8[2.13], and mechanic 2.0[3.82]. Cigarette smoking contributed substantially to personal exposures, approximately doubling PM2.5 and OC, but having less of an effect on EC. Large differences were seen across the terminal sites due to differences in local regional air pollution levels from traffic and other sources. We conclude that it will be possible to estimate current exposures of the cohort using an exposure assignment matrix based on job title, work location, and terminal site. This distribution overlaps substantially with the general public's exposure to these sources.

3D-information fusion from very high resolution satellite sensors

NASA Astrophysics Data System (ADS)

Krauss, T.; d'Angelo, P.; Kuschk, G.; Tian, J.; Partovi, T.

2015-04-01

In this paper we show the pre-processing and potential for environmental applications of very high resolution (VHR) satellite stereo imagery like these from WorldView-2 or Pl'eiades with ground sampling distances (GSD) of half a metre to a metre. To process such data first a dense digital surface model (DSM) has to be generated. Afterwards from this a digital terrain model (DTM) representing the ground and a so called normalized digital elevation model (nDEM) representing off-ground objects are derived. Combining these elevation based data with a spectral classification allows detection and extraction of objects from the satellite scenes. Beside the object extraction also the DSM and DTM can directly be used for simulation and monitoring of environmental issues. Examples are the simulation of floodings, building-volume and people estimation, simulation of noise from roads, wave-propagation for cellphones, wind and light for estimating renewable energy sources, 3D change detection, earthquake preparedness and crisis relief, urban development and sprawl of informal settlements and much more. Also outside of urban areas volume information brings literally a new dimension to earth oberservation tasks like the volume estimations of forests and illegal logging, volume of (illegal) open pit mining activities, estimation of flooding or tsunami risks, dike planning, etc. In this paper we present the preprocessing from the original level-1 satellite data to digital surface models (DSMs), corresponding VHR ortho images and derived digital terrain models (DTMs). From these components we present how a monitoring and decision fusion based 3D change detection can be realized by using different acquisitions. The results are analyzed and assessed to derive quality parameters for the presented method. Finally the usability of 3D information fusion from VHR satellite imagery is discussed and evaluated.

Novel method for detection of glycogen in cells.

PubMed

Skurat, Alexander V; Segvich, Dyann M; DePaoli-Roach, Anna A; Roach, Peter J

2017-05-01

Glycogen, a branched polymer of glucose, functions as an energy reserve in many living organisms. Abnormalities in glycogen metabolism, usually excessive accumulation, can be caused genetically, most often through mutation of the enzymes directly involved in synthesis and degradation of the polymer leading to a variety of glycogen storage diseases (GSDs). Microscopic visualization of glycogen deposits in cells and tissues is important for the study of normal glycogen metabolism as well as diagnosis of GSDs. Here, we describe a method for the detection of glycogen using a renewable, recombinant protein which contains the carbohydrate-binding module (CBM) from starch-binding domain containing protein 1 (Stbd1). We generated a fusion protein containing g lutathione S-transferase, a cM c eptitope and the tbd1 BM (GYSC) for use as a glycogen-binding probe, which can be detected with secondary antibodies against glutathione S-transferase or cMyc. By enzyme-linked immunosorbent assay, we demonstrate that GYSC binds glycogen and two other polymers of glucose, amylopectin and amylose. Immunofluorescence staining of cultured cells indicate a GYSC-specific signal that is co-localized with signals obtained with anti-glycogen or anti-glycogen synthase antibodies. GYSC-positive staining inside of lysosomes is observed in individual muscle fibers isolated from mice deficient in lysosomal enzyme acid alpha-glucosidase, a well-characterized model of GSD II (Pompe disease). Co-localized GYSC and glycogen signals are also found in muscle fibers isolated from mice deficient in malin, a model for Lafora disease. These data indicate that GYSC is a novel probe that can be used to study glycogen metabolism under normal and pathological conditions. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Ultrafine particle concentrations and exposures in seven residences in northern California.

PubMed

Bhangar, S; Mullen, N A; Hering, S V; Kreisberg, N M; Nazaroff, W W

2011-04-01

Human exposures to ultrafine particles (UFP) are poorly characterized given the potential associated health risks. Residences are important sites of exposure. To characterize residential exposures to UFP in some circumstances and to investigate governing factors, seven single-family houses in California were studied during 2007-2009. During multiday periods, time-resolved particle number concentrations were monitored indoors and outdoors and information was acquired concerning occupancy, source-related activities, and building operation. On average, occupants were home for 70% of their time. The geometric mean time-average residential exposure concentration for 21 study subjects was 14,500 particles per cm(3) (GSD = 1.8; arithmetic mean ± standard deviation = 17,000 ± 10,300 particles per cm(3)). The average contribution to residential exposures from indoor episodic sources was 150% of the contribution from particles of outdoor origin. Unvented natural-gas pilot lights contributed up to 19% to exposure for the two households where present. Episodic indoor source activities, most notably cooking, caused the highest peak exposures and most of the variation in exposure among houses. Owing to the importance of indoor sources and variations in the infiltration factor, residential exposure to UFP cannot be characterized by ambient measurements alone. Indoor and outdoor sources each contribute to residential ultrafine particle (UFP) concentrations and exposures. Under the conditions investigated, peak exposure concentrations indoors were associated with cooking, using candles, or the use of a furnace. Active particle removal systems can mitigate exposure by reducing the persistence of particles indoors. Eliminating the use of unvented gas pilot lights on cooking appliances could also be beneficial. The study results indicate that characterization of human exposure to UFP, an air pollutant of emerging public health concern, cannot be accomplished without a good understanding of conditions inside residences. © 2010 John Wiley & Sons A/S.

Comparison and Analysis of Accuracy of Elevation Extraction Based on the ZY-3 01 and 02 Satellites Stereoscopic Images

NASA Astrophysics Data System (ADS)

Zhao, L.; Fu, X.; Dou, X.; Liu, H.; Fang, Z.

2018-04-01

The ZY-3 is the civil high-resolution optical stereoscopic mapping satellite independently developed by China. The ZY-3 constellation of the twin satellites operates in a sun-synchronous, near-polar, circular 505 km orbit, with a descending location time of 10:30 AM and a 29-day revisiting period. The panchromatic triplet sensors, pointing forward, nadir, and backward with an angle of 22°, have excellent base-to-height ratio, which is beneficial to the extraction of DEM. In order to extract more detailed and highprecision DEM, the ZY-3 (02) satellite has been upgraded based on the ZY-3 (01), and the GSD of the stereo camera has been optimized from 3.5 to 2.5 meters. In the paper case studies using the ZY-3 01 and the 02 satellite data for block adjustment and DEM extraction have been carried out in Liaoning Province of China. The results show that the planimetric and altimetric accuracy can reach 3 meters, which meet the mapping requirements of 1 : 50,000 national topographic map and the design performance of the satellites. The normalized elevation accuracy index (NEAI) is adopted to evaluate the twin satellite stereoscopic performance, and the NEAIs of the twin ZY-3 satellites are good and the index of the ZY-3(02) is slightly better. The comparison of the overlapping DEMs from the twin ZY-3 satellites and SRTM is analysed. The bias and the standard deviation of all the DEMs are better than 5 meters. In addition, in the process of accuracy comparison, some gross errors of the DEM can be identified, and some elevation changes of the DEM can also be found. The differential DEM becomes a new tool and application.

A comparison of performance of automatic cloud coverage assessment algorithm for Formosat-2 image using clustering-based and spatial thresholding methods

NASA Astrophysics Data System (ADS)

Hsu, Kuo-Hsien

2012-11-01

Formosat-2 image is a kind of high-spatial-resolution (2 meters GSD) remote sensing satellite data, which includes one panchromatic band and four multispectral bands (Blue, Green, Red, near-infrared). An essential sector in the daily processing of received Formosat-2 image is to estimate the cloud statistic of image using Automatic Cloud Coverage Assessment (ACCA) algorithm. The information of cloud statistic of image is subsequently recorded as an important metadata for image product catalog. In this paper, we propose an ACCA method with two consecutive stages: preprocessing and post-processing analysis. For pre-processing analysis, the un-supervised K-means classification, Sobel's method, thresholding method, non-cloudy pixels reexamination, and cross-band filter method are implemented in sequence for cloud statistic determination. For post-processing analysis, Box-Counting fractal method is implemented. In other words, the cloud statistic is firstly determined via pre-processing analysis, the correctness of cloud statistic of image of different spectral band is eventually cross-examined qualitatively and quantitatively via post-processing analysis. The selection of an appropriate thresholding method is very critical to the result of ACCA method. Therefore, in this work, We firstly conduct a series of experiments of the clustering-based and spatial thresholding methods that include Otsu's, Local Entropy(LE), Joint Entropy(JE), Global Entropy(GE), and Global Relative Entropy(GRE) method, for performance comparison. The result shows that Otsu's and GE methods both perform better than others for Formosat-2 image. Additionally, our proposed ACCA method by selecting Otsu's method as the threshoding method has successfully extracted the cloudy pixels of Formosat-2 image for accurate cloud statistic estimation.

International Commercial Remote Sensing Practices and Policies: A Comparative Analysis

NASA Astrophysics Data System (ADS)

Stryker, Timothy

In recent years, there has been much discussion about U.S. commercial remoteUnder the Act, the Secretary of Commerce sensing policies and how effectively theylicenses the operations of private U.S. address U.S. national security, foreignremote sensing satellite systems, in policy, commercial, and public interests.consultation with the Secretaries of Defense, This paper will provide an overview of U.S.State, and Interior. PDD-23 provided further commercial remote sensing laws,details concerning the operation of advanced regulations, and policies, and describe recentsystems, as well as criteria for the export of NOAA initiatives. It will also addressturnkey systems and/or components. In July related foreign practices, and the overall2000, pursuant to the authority delegated to legal context for trade and investment in thisit by the Secretary of Commerce, NOAA critical industry.iss ued new regulations for the industry. Licensing and Regulationsatellite systems. NOAA's program is The 1992 Land Remote Sensing Policy Act ("the Act"), and the 1994 policy on Foreign Access to Remote Sensing Space Capabilities (known as Presidential Decision Directive-23, or PDD-23) put into place an ambitious legal and policy framework for the U.S. Government's licensing of privately-owned, high-resolution satellite systems. Previously, capabilities afforded national security and observes the international obligations of the United States; maintain positive control of spacecraft operations; maintain a tasking record in conjunction with other record-keeping requirements; provide U.S. Government access to and use of data when required for national security or foreign policy purposes; provide for U.S. Government review of all significant foreign agreements; obtain U.S. Government approval for any encryption devices used; make available unenhanced data to a "sensed state" as soon as such data are available and on reasonable cost terms and conditions; make available unenhanced data as requested by the U.S. Government Archive; and, obtain a priori U.S. Government approval of all plans and procedures to deal with safe disposition of the satellite. Further information on NOAA's regulations and NOAA's licensing program is available at www.licensing.noaa.gov. Monitoring and Enforcement NOAA's enforcement mission is focused on the legislative mandate which states that the Secretary of Commerce has a continuing obligation to ensure that licensed imaging systems are operated lawfully to preserve the national security and foreign policies of the United States. NOAA has constructed an end-to-end monitoring and compliance program to review the activities of licensed companies. This program includes a pre- launch review, an operational baseline audit, and an annual comprehensive national security audit. If at any time there is suspicion or concern that a system is being operated unlawfully, a no-notice inspection may be initiated. setbacks, three U.S. companies are now operational, with more firms expected to become so in the future. While NOAA does not disclose specific systems capabilities for proprietary reasons, its current licensing resolution thresholds for general commercial availability are as follows: 0.5 meter Ground Sample Distance (GSD) for panchromatic systems, 2 meter GSD for multi-spectral systems, 3 meter Impulse Response (IPR) for Synthetic Aperture Radar systems, and 20 meter GSD for hyperspectral systems (with certain 8-meter hyperspectral derived products also licensed for commercial distribution). These thresholds are subject to change based upon foreign availability and other considerations. It should also be noted that license applications are reviewed and granted on a case-by-case basis, pursuant to each system's technology and concept of operations. In 2001, NOAA, along with the Department of Commerce's International Trade Administration, commissioned a study by the RAND Corporation to assess the risks faced by the U.S. commercial remote sensing satellite industry. In commissioning this study, NOAA's goal was to better understand the role that U.S. Government policies and regulations have in shaping the prospects for emerging commercial remote sensing satellite firms. The study assessed the risks against broader trends in the larger U.S. remote sensing industry and geospatial technology and effective policy implementation. The Department of Commerce is working with NOAA licensees to identify foreign actions which could restrict market access by U.S. firms, and seeking to provide a "level playing field" for U.S. service providers. The Department of Commerce has dedicated new resources to its licensing activities. In Fiscal Year 2002, the Department obtained 1.2 million in funding to support the NOAA program, through staff, equipment, technical support, constituent outreach, and market and policy studies. To better understand the market and make more well-informed licensing decisions, NOAA is participating in a broad-based market study effort under the direction of the American Society for Photogrammetry and Remote Sensing (ASPRS) and NASA's Commercial Remote Sensing Program. This study is providing long-term analysis of the commercial remote sensing industry. It is being supported by interviews with industry and government experts, a web-based survey, and a thorough review and analysis of related literature. The project should more clearly determine future remote sensing needs and requirements, and maximize the industry's baselines, standards, and socio-economic potential. NOAA, through its participation in this study, has gained important new insights into the status and future trends of this industry. The study's initial findings estimate 2001 industry revenue at 2 billion, growing at 13% per year, to an approximate level of 6 billion in 2010 (in constant, calendar year 2000 dollars). Currently, across all sectors, the most active market segments are in nati onal /glo bal security, mapping/geography, civil government, and have provided for appropriate measures for monitoring and compliance. This approach provides a valuable framework for companies, investors, customers, and foreign partners. The clearly-defined ground rules are designed to facilitate full private sector competition, innovation, and domestic and international market development. International market development remains a key issue for the U.S. Government and for U.S. industry in general. NOAA has learned of some interest by foreign governments in promulgating new laws and regulations to address this growing industry. However, to date, most governments have yet to publicize new commercial remote sensing laws or regulations. In some instances, data policies for commercial remote sensing have been developed, but only in the context of government-owned and operated systems, or private systems in which a government is the controlling shareholder. Other than some initial consultations and limited agreements between supplier nations, there has to date been little overall international coordination of commercial remote sensing policies and practices. The result has been an uncertain and non- uniform international business environment, which can cause difficulties for all commercial remote sensing operators. Related international market distortions inhibit the maturation of the industry and the normalization of business practices. This situation may make it more difficult for key stakeholders to make decisions on investments, purchases, regulatory affairs, and international partnerships. To put this growing industry on a more level footing, there should be further coordination

Tracking the Creation of Tropical Forest Canopy Gaps with UAV Computer Vision Remote Sensing

NASA Astrophysics Data System (ADS)

Dandois, J. P.

2015-12-01

The formation of canopy gaps is fundamental for shaping forest structure and is an important component of ecosystem function. Recent time-series of airborne LIDAR have shown great promise for improving understanding of the spatial distribution and size of forest gaps. However, such work typically looks at gap formation across multiple years and important intra-annual variation in gap dynamics remains unknown. Here we present findings on the intra-annual dynamics of canopy gap formation within the 50 ha forest dynamics plot of Barro Colorado Island (BCI), Panama based on unmanned aerial vehicle (UAV) remote sensing. High-resolution imagery (7 cm GSD) over the 50 ha plot was obtained regularly (≈ every 10 days) beginning October 2014 using a UAV equipped with a point and shoot camera. Imagery was processed into three-dimensional (3D) digital surface models (DSMs) using automated computer vision structure from motion / photogrammetric methods. New gaps that formed between each UAV flight were identified by subtracting DSMs between each interval and identifying areas of large deviation. A total of 48 new gaps were detected from 2014-10-02 to 2015-07-23, with sizes ranging from less than 20 m2 to greater than 350 m2. The creation of new gaps was also evaluated across wet and dry seasons with 4.5 new gaps detected per month in the dry season (Jan. - May) and 5.2 per month outside the dry season (Oct. - Jan. & May - July). The incidence of gap formation was positively correlated with ground-surveyed liana stem density (R2 = 0.77, p < 0.001) at the 1 hectare scale. Further research will consider the role of climate in predicting gap formation frequency as well as site history and other edaphic factors. Future satellite missions capable of observing vegetation structure at greater extents and frequencies than airborne observations will be greatly enhanced by the high spatial and temporal resolution bridging scale made possible by UAV remote sensing.

Use of Visible Satellite Imagery to Determine Velocity in Tidal Rivers

NASA Astrophysics Data System (ADS)

Mied, R. P.; Donato, T. F.; Chen, W.

2006-05-01

In the open ocean and on the continental shelf, current velocities have traditionally been calculated remotely using the Maximum Correlation Coefficient (MCC) technique to track features between sequential sea surface temperature image scenes. These images are obtained from NOAA polar orbiters having an effective ground pixel size of 1.47 km. In contrast to this relatively large distance, spatial scales over which current velocities can vary in rivers and estuaries are hundreds of meters; associated temporal scales vary from tens of minutes to hours. Traditional in-situ measurements can be instructive in determining some aspects of the flow, but truly synoptic overviews are possible only with remote sensing, provided high-resolution imagery is available. With the advent of a constellation of moderate- to high-resolution imaging systems (e.g., Landsat, ASTER, SPOT, Quickbird, Ikonos, and Orbview-3) it is now available to extend current estimations to these areas. For instance, Landsat-7 and ASTER produce imagery with spatial resolutions on the order of 30 m or less and within 30 min of each other. This is sufficient to spatially resolve a wide variety of surface features, and to maintain feature integrity over time for tracking purposes. We apply this approach to a portion of the tidal Potomac River by using pairs of co-registered, sequential, multi-spectral Landsat-7 and ASTER images. The final data used in the analysis set contain three spectral bands (green, red, and near-infrared), and have a ground pixel spacing (GSD) of 30m. The time step between each Landsat-7 and ASTER pair is approximately 29 minutes. Two image sets are used in the present study, one occurring on 5 October 2001 and the other on 2 April 2003. We show current maps derived from both image pairs an discuss the results in the light of model and

Combining Imagery and Models to Understand River Dynamics

NASA Astrophysics Data System (ADS)

Blain, C. A.; Mied, R. P.; Linzell, R. S.

2014-12-01

Rivers pose one of the most challenging environments to characterize. Their geometric complexity and continually changing position and character are difficult to measure under optimal circumstances. Further compounding the problem is the often inaccessibility of these areas around the globe. Yet details of the river bank position and bed elevation are essential elements in the construction of accurate predictive river models. To meet this challenge, remote sensing imagery is first used to initialize the construction of advanced high resolution river circulation models. In turn, such models are applied to dynamically interpret remotely-sensed surface features. A method has been developed to automatically extract water and shoreline locations from arbitrarily sourced high resolution (~1m gsd) visual spectrum imagery without recourse to the spectral or color information. The approach relies on quantifying the difference in image texture between the relatively smooth water surface and the comparatively rough surface of surrounding land. Processing the segmented land/water interface results in ordered, continuous shoreline coordinates that bound river model construction. In the absence of observed bed elevations, one of several available analytic bathymetry cross-sectional relations are applied to complete the river model configuration. Successful application of this approach to the Snohomish River, WA and the Pearl River, MS are demonstrated. Once constructed, a hydrodynamic model of the river model can also be applied to unravel the dynamics responsible for observed surface features in the imagery. At a creek-river confluence in the Potomac River, MD, an ebb tide front observed in the imagery is analyzed using the model. The result is knowledge that an ebb shoal located just outside of the creek must be present and is essential for front formation. Furthermore, the front is found to be persistent throughout the tidal cycle, although it changes sign between ebb and flood phases. The presence of the creek only minimally modifies the underlying currents.

VISTA - A Constellation for Real Time Regional Imaging

NASA Astrophysics Data System (ADS)

Meerman, Max; Boland, Lee; da Silva Curiel, Alex; Sweeting, Martin, , Sir

2002-01-01

The role of satellites in medium and high-resolution reconnaissance of the Earth's surface has been well demonstrated in recent years through missions such as Landsat, SPOT, IKONOS, ImageSat and Quickbird. The market for such data products is well served and likely to become more competitive with further very-high-resolution missions. Whereas commercial markets have concentrated on enhancing resolution, the small satellite sector has concentrated on reducing the cost of data products, and the development of systems providing niche services. One such EO requirement that can be well met by smaller satellites is the need for higher temporal resolution, as this typically requires a large number of satellites to operate as a constellation - thus far financially impractical using conventional EO satellites. Surrey is currently engaged in building its first constellation that will provide daily global coverage at moderate resolution (32-metre GSD and 600km swath) in three spectral bands. Targeted at providing timely quick-look data products for disaster mitigation and monitoring, the constellation comprises 7 satellites in a single orbital plane. Each satellite has a wide swath so that successive satellites progressively cover the entire globe in a single day. The Vista constellation takes this concept a step further, and is proposed for applications requiring near-continuous surveillance of regional activity. By introducing a multiple plane constellation of small Earth observation satellites, it is possible to monitor continuously selected regions anywhere on the globe. The paper describes the system trades and outlines the scope of the performance that could be obtained from such a system. A cost model illustrates that the balance between launch and space segment costs must be reached by considering suitable replacement strategies, and that the system is highly sensitive to requirement creep. Finally, it is shown that the use of cost effective, small satellites leads to solutions previously thought to be financially beyond sensible reach.

Disruption of apoptosis pathways involved in zebrafish gonad differentiation by 17α-ethinylestradiol and fadrozole exposures.

PubMed

Luzio, Ana; Matos, Manuela; Santos, Dércia; Fontaínhas-Fernandes, António A; Monteiro, Sandra M; Coimbra, Ana M

2016-08-01

Zebrafish (Danio rerio) sex determination seems to involve genetic factors (GSD) but also environmental factors (ESD), such as endocrine disrupting chemicals (EDCs) that are known to mimic endogenous hormones and disrupt gonad differentiation. Apoptosis has also been proposed to play a crucial role in zebrafish gonad differentiation. Nevertheless, the interactions between EDCs and apoptosis have received little attention. Thus, this study aimed to assess if and which apoptotic pathways are involved in zebrafish gonad differentiation and how EDCs may interfere with this process. With these purposes, zebrafish were exposed to 17α-ethinylestradiol (EE2, 4ng/L) and fadrozole (Fad, 50μg/L) from 2h to 35days post-fertilization (dpf). Afterwards, a gene expression analysis by qRT-PCR and a stereological analysis, based on systematic sampling and protein immunohistochemistry, were performed. The death receptors (FAS; TRADD), anti-apoptotic (BCL-2; MDM2), pro-apoptotic (CASP-2 and -6) and cell proliferation (BIRC5/survivin; JUN) genes and proteins were evaluated. In general, apoptosis was inhibited in females through the involvement of anti-apoptotic pathways, while in males apoptosis seemed to be crucial to the failure of the "juvenile ovary" development and the induction of testes transformation. The JUN protein was shown to be necessary in juvenile ovaries, while the BIRC5 protein seemed to be involved in zebrafish spermatogenesis. Both EDCs, EE2 and Fad, increased the apoptosis stimulus in zebrafish gonad. It was noticed that the few females that were resistant to Fad-induced sex reversal had increased anti-apoptotic factor levels, while males exposed to EE2 showed increased pro-apoptotic genes/proteins and were more advanced in gonad differentiation. Overall, our findings show that apoptosis pathways are involved in zebrafish gonad differentiation and that EDCs can disrupt this process. Copyright © 2016 Elsevier B.V. All rights reserved.

Inhalation carcinogenicity study with nickel metal powder in Wistar rats

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oller, Adriana R.; Kirkpatrick, Daniel T.; Radovsky, Ann

Epidemiological studies of nickel refinery workers have demonstrated an association between increased respiratory cancer risk and exposure to certain nickel compounds (later confirmed in animal studies). However, the lack of an association found in epidemiological analyses for nickel metal remained unconfirmed for lack of robust animal inhalation studies. In the present study, Wistar rats were exposed by whole-body inhalation to 0, 0.1, 0.4, and 1.0 mg Ni/m{sup 3} nickel metal powder (MMAD = 1.8 {mu}m, GSD = 2.4 {mu}m) for 6 h/day, 5 days/week for up to 24 months. A subsequent six-month period without exposures preceded the final euthanasia. Highmore » mortality among rats exposed to 1.0 mg Ni/m{sup 3} nickel metal resulted in the earlier termination of exposures in this group. The exposure level of 0.4 mg Ni/m{sup 3} was established as the MTD for the study. Lung alterations associated with nickel metal exposure included alveolar proteinosis, alveolar histiocytosis, chronic inflammation, and bronchiolar-alveolar hyperplasia. No increased incidence of neoplasm of the respiratory tract was observed. Adrenal gland pheochromocytomas (benign and malignant) in males and combined cortical adenomas/carcinomas in females were induced in a dose-dependent manner by the nickel metal exposure. The incidence of pheochromocytomas was statistically increased in the 0.4 mg Ni/m{sup 3} male group. Pheochromocytomas appear to be secondary to the lung toxicity associated with the exposure rather than being related to a direct nickel effect on the adrenal glands. The incidence of cortical tumors among 0.4 mg Ni/m{sup 3} females, although statistically higher compared to the concurrent controls, falls within the historical control range; therefore, in the present study, this tumor is of uncertain relationship to nickel metal exposure. The lack of respiratory tumors in the present animal study is consistent with the findings of the epidemiological studies.« less

SW-MW infrared spectrometer for lunar mission

NASA Astrophysics Data System (ADS)

Banerjee, Arup; Biswas, Amiya; Joshi, Shaunak; Kumar, Ankush; Rehman, Sami; Sharma, Satish; Somani, Sandip; Bhati, Sunil; Karelia, Jitendra; Saxena, Anish; Chowdhury, Arup R.

2016-04-01

SW-MW Imaging Infrared Spectrometer, the Hyperspectral optical imaging instrument is envisaged to map geomorphology and mineralogy of lunar surface. The instrument is designed to image the electro-magnetic energy emanating from moon's surface with high spectral and spatial resolution for the mission duration from an altitude of 100 km. It is designed to cover 0.8 to 5 μm in 250 spectral bands with GSD 80m and swath 20km. Primarily, there are three basic optical segments in the spectrometer. They are fore optics, dispersing element and focusing elements. The payload is designed around a custom developed multi-blaze convex grating optimized for system throughput. The considerations for optimization are lunar radiation, instrument background, optical throughput, and detector sensitivity. HgCdTe (cooled using a rotary stirling cooler) based detector array (500x256 elements, 30μm) is being custom developed for the spectrometer. Stray light background flux is minimized using a multi-band filter cooled to cryogenic temperature. Mechanical system realization is being performed considering requirements such as structural, opto-mechanical, thermal, and alignment. The entire EOM is planned to be maintained at 240K to reduce and control instrument background. Al based mirror, grating, and EOM housing is being developed to maintain structural requirements along with opto- mechanical and thermal. Multi-tier radiative isolation and multi-stage radiative cooling approach is selected for maintaining the EOM temperature. EOM along with precision electronics packages are planned to be placed on the outer and inner side of Anti-sun side (ASS) deck. Power and Cooler drive electronics packages are planned to be placed on bottom side of ASS panel. Cooler drive electronics is being custom developed to maintain the detector temperature within 100mK during the imaging phase. Low noise detector electronics development is critical for maintaining the NETD requirements at different target temperatures. Subsequent segments of the paper bring out system design aspects and trade-off analyses.

The 2015 super-resolution microscopy roadmap

NASA Astrophysics Data System (ADS)

Hell, Stefan W.; Sahl, Steffen J.; Bates, Mark; Zhuang, Xiaowei; Heintzmann, Rainer; Booth, Martin J.; Bewersdorf, Joerg; Shtengel, Gleb; Hess, Harald; Tinnefeld, Philip; Honigmann, Alf; Jakobs, Stefan; Testa, Ilaria; Cognet, Laurent; Lounis, Brahim; Ewers, Helge; Davis, Simon J.; Eggeling, Christian; Klenerman, David; Willig, Katrin I.; Vicidomini, Giuseppe; Castello, Marco; Diaspro, Alberto; Cordes, Thorben

2015-11-01

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms. Unfortunately, the applicability of the optical microscope is limited, since the diffraction of light imposes limitations on the spatial resolution of the image. Consequently the details of, for example, cellular protein distributions, can be visualized only to a certain extent. Fortunately, recent years have witnessed the development of ‘super-resolution’ far-field optical microscopy (nanoscopy) techniques such as stimulated emission depletion (STED), ground state depletion (GSD), reversible saturated optical (fluorescence) transitions (RESOLFT), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM) or saturated structured illumination microscopy (SSIM), all in one way or another addressing the problem of the limited spatial resolution of far-field optical microscopy. While SIM achieves a two-fold improvement in spatial resolution compared to conventional optical microscopy, STED, RESOLFT, PALM/STORM, or SSIM have all gone beyond, pushing the limits of optical image resolution to the nanometer scale. Consequently, all super-resolution techniques open new avenues of biomedical research. Because the field is so young, the potential capabilities of different super-resolution microscopy approaches have yet to be fully explored, and uncertainties remain when considering the best choice of methodology. Thus, even for experts, the road to the future is sometimes shrouded in mist. The super-resolution optical microscopy roadmap of Journal of Physics D: Applied Physics addresses this need for clarity. It provides guidance to the outstanding questions through a collection of short review articles from experts in the field, giving a thorough discussion on the concepts underlying super-resolution optical microscopy, the potential of different approaches, the importance of label optimization (such as reversible photoswitchable proteins) and applications in which these methods will have a significant impact. Mark Bates, Christian Eggeling

Application of Unmanned Aerial System-based Photogrammetry to Monitor Landforms Evolution of Mudstone Badlands

NASA Astrophysics Data System (ADS)

Chen, Yichin

2017-04-01

Mudstone badlands are the area characteristized by its rapid erosion and steep, fractured, and barren landforms. Monitoring the topography changes in badland help improve our knowledge of the hillslope and river processing on landforms and develop susceptibility model for surface erosion hazards. Recently, advances in unmanned aerial system (UAS) and close-range photogrammetry technology have opened up the possibility of effectively measuring topography changes with high spatiotemporal resolutions. In this study, we used the UAS and close-range photogrammetry technology to monitor the topography changes in a rapidly eroded badland, south-western Taiwan. A small mudstone hillslope with area of 0.2 ha approximately and with slope gradient of 37 degrees was selected as the study site. A widely used and commercial quadcopter equipped non-metric camera was used to take images with ground sampling distance (GSD) 5 mm approximately. The Pix4DMapper, a commercial close-range photogrammetry software, was used to perform stereo matching, extract point clouds, generate digital surface models (DSMs) and orthoimage. To control model accuracy, a set of ground control points was surveyed by using eGPS. The monitoring was carried out after every significant rainfall event that may induced observable erosion in the badland site. The results show that DSMs have the GSDs of 4.0 5.4 mm and vertical accuracy of 61 116 mm. The accuracy largely depends on the quality of ground control points. The spatial averaged erosion rate during six months of monitoring was 328 mm, which is higher in the gully sides than in the ridges. The erosion rate is positively correlated with the slope gradient and drainage contributing area that implies the important role of surface gully erosion in mudstone badland erosion. This study shows that UAS and close-range photogrammetry technology can be used to monitor the topography change in badland areas effectively and can provide high spatiotemporal resolutions of DSMs for developing distributed surface erosion models.

Tunneling topological vacua via extended operators: (Spin-)TQFT spectra and boundary deconfinement in various dimensions

NASA Astrophysics Data System (ADS)

Wang, Juven; Ohmori, Kantaro; Putrov, Pavel; Zheng, Yunqin; Wan, Zheyan; Guo, Meng; Lin, Hai; Gao, Peng; Yau, Shing-Tung

2018-05-01

Distinct quantum vacua of topologically ordered states can be tunneled into each other via extended operators. The possible applications include condensed matter and quantum cosmology. We present a straightforward approach to calculate the partition function on various manifolds and ground state degeneracy (GSD), mainly based on continuum/cochain topological quantum field theories (TQFTs), in any dimension. This information can be related to the counting of extended operators of bosonic/fermionic TQFTs. On the lattice scale, anyonic particles/strings live at the ends of line/surface operators. Certain systems in different dimensions are related to each other through dimensional reduction schemes, analogous to (de)categorification. Examples include spin TQFTs derived from gauging the interacting fermionic symmetry-protected topological states (with fermion parity {Z}_2^f) of symmetry groups {Z}_4× {Z}_2 and ({Z}_4)^2 in 3+1D, also {Z}_2 and ({Z}_2)^2 in 2+1D. Gauging the last three cases begets non-Abelian spin TQFTs (fermionic topological order). We consider situations where a TQFT lives on (1) a closed spacetime or (2) a spacetime with a boundary, such that the bulk and boundary are fully gapped and short- or long-range entangled (SRE/LRE). Anyonic excitations can be deconfined on the boundary. We introduce new exotic topological interfaces on which neither particle nor string excitations alone condense, but only fuzzy-composite objects of extended operators can end (e.g., a string-like composite object formed by a set of particles can end on a special 2+1D boundary of 3+1D bulk). We explore the relations between group extension constructions and partially breaking constructions (e.g., 0-form/higher-form/"composite" breaking) of topological boundaries, after gauging. We comment on the implications of entanglement entropy for some such LRE systems.

Sunsat-2004 satellite and synoptic VLF payload

NASA Astrophysics Data System (ADS)

Milne, Gw; Hughes, A.; Mostert, S.; Steyn, Wh

Sunsat 2004 is a second satellite from the University of Stellenbosch, with intended suns-synchronous launch in late 2005. The first satellite, Sunsat, was launched in February 1999, and was Africa's first satellite The three-axis stabilised bus will normally point its main solar panel at the sun, but will rotate for imaging. The attitude determination and control system will use coarse sun sensors, magnetometers, rate gyros, and a star mapper, and use reaction wheels and torquer rods for actuation. The payloads include a multispectral pushbroom imager with less than 5m GSD, TV cameras, an Amateur Radio communications payload, and science experiments. The main South African science experiment is a VLF receiver. In the magnetosphere VLF waves play an important role in energy exchange processes with energetic particles. The wave-particle interactions can lead to particle precipitation into the atmosphere or introduce additional energy into particle populations in the magnetosphere. The former is important due to its effect on terrestrial communications while the latter is of interest, as it affects the environment in which satellites operate. A full understanding, of the magnetosphere and phenomena such as the aurora, airglow and particle precipitation, depends on comprehensive wave and particle models together with models of the background plasma density The energetic particle populations and background plasma densities have been extensively modelled using data from a large number of satellite, rocket and ground-based experiments but no comprehensive model of the wave environment exist. The proposed synoptic VLF experiment will start to address this need by locating and tracking the morphology of regions in the magnetosphere where waves are generated. The experiment would consist of a nine channel VLF receiver with a loop antenna. The data would be recorded on board and transmitted to ground stations at appropriate times. A number of additional science payloads are also being evaluated for the mission, and will be reported on in the paper.

Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex.

PubMed

Toyota, Kenji; Miyakawa, Hitoshi; Hiruta, Chizue; Furuta, Kenjiro; Ogino, Yukiko; Shinoda, Tetsuro; Tatarazako, Norihisa; Miyagawa, Shinichi; Shaw, Joseph R; Iguchi, Taisen

2015-09-01

Sex-determination systems can be divided into two groups: genotypic sex determination (GSD) and environmental sex determination (ESD). ESD is an adaptive life-history strategy that allows control of sex in response to environmental cues in order to optimize fitness. However, the molecular basis of ESD remains largely unknown. The micro crustacean Daphnia pulex exhibits ESD in response to various external stimuli. Although methyl farnesoate (MF: putative juvenile hormone, JH, in daphnids) has been reported to induce male production in daphnids, the role of MF as a sex-determining factor remains elusive due to the lack of a suitable model system for its study. Here, we establish such a system for ESD studies in D. pulex. The WTN6 strain switches from producing females to producing males in response to the shortened day condition, while the MFP strain only produces females, irrespective of day-length. To clarify whether MF has a novel physiological role as a sex-determining factor in D. pulex, we demonstrate that a MF/JH biosynthesis inhibitor suppressed male production in WTN6 strain reared under the male-inducible condition, shortened day-length. Moreover, we show that juvenile hormone acid O-methyltransferase (JHAMT), a critical enzyme of MF/JH biosynthesis, displays MF-generating activity by catalyzing farnesoic acid. Expression of the JHAMT gene increased significantly just before the MF-sensitive period for male production in the WTN6 strain, but not in the MFP strain, when maintained under male-inducible conditions. These results suggest that MF synthesis regulated by JHAMT is necessary for male offspring production in D. pulex. Our findings provide novel insights into the genetic underpinnings of ESD and they begin to shed light on the physiological function of MF as a male-fate determiner in D. pulex. Copyright © 2015 Elsevier Ltd. All rights reserved.

Radiocaesium levels in game in the Czech Republic.

PubMed

Škrkal, Jan; Rulík, Petr; Fantínová, Karin; Mihalík, Ján; Timková, Jana

2015-01-01

The paper summarizes results of monitoring of (137)Cs activities in game species roaming in the woods over the territory of the Czech Republic for the time interval of 1986-2012. Geometric means and other statistical characteristics were estimated from the data sets on the assumption of log-normal distribution of the data from the time interval 2004-2012 where the character of data distribution had displayed no significant change. Geometric means (in Bq/kg) in meat were: wild boar 5.1, red deer 1.9, roe deer 0.77 and feathered game 0.14. The mean value in the less frequent game amounted to 0.36 Bq/kg. The geometrical standard deviation (GSD) widely varied from 1.6 to 21 for the studied species. Based on mass activity dependence on time, we assessed the effective and environmental half-lives of activity decline. For red deer and roe deer, the effective (137)Cs half-life was 2.9 and 3.2 years, and environmental half-life 3.2 and 3.6 years respectively. The effective half-life of (137)Cs in wild boar of 38 years was determined with large uncertainty and it shows constant influx of (137)Cs activity to the digestive tract of wild boars. A statistically significant season-based (137)Cs level was found in red deer and wild boar. Higher winter and spring activities of (137)Cs in wild boar are linked with decreasing access to naturally occurring food with lower (137)Cs content (chestnuts, acorns, and beech nuts), making boar grub around for ground-deposited food (often for mushrooms with higher activity). Higher winter activities of (137)Cs in red deer meat, most probably, are due to lower access to green diet in winter. The average annual committed effective dose for Czech population based on estimates of game species meat consumption between 2004 and 2012 was insignificant, only 0.03 μSv. Copyright © 2014 Elsevier Ltd. All rights reserved.

Endotoxin exposure and changes in short-term pulmonary function among sewage workers.

PubMed

Cyprowski, Marcin; Sobala, Wojciech; Buczyńska, Alina; Szadkowska-Stańczyk, Irena

2015-01-01

The inhaled endotoxin is considered as a causative factor in the process of acute bronchial obstruction, which can be measured by a decrease in forced expiratory volume in 1 s (FEV1). The aim of this study was to assess endotoxin exposure among sewage treatment plant workers (STPW) and its effect on across-shift changes in respiratory airflow. A group of 78 STPW from a large sewage treatment plant was studied. Inhalable dust for endotoxin assessment was collected using personal aerosol samplers. Endotoxin was assayed with the kinetic, chromogenic Limulus amebocyte lysate test. Across-shift spirometric measurements were performed on Mondays, after 2-days absence from work, with the use of portable spirometer. The forced vital capacity (FVC), and FEV1 parameters were analyzed. Multifactor regression modeling was performed to determine parameters significantly associated with endotoxin exposure. The concentration of inhalable dust and endotoxin ranged from 0.01-1.38 mg/m3 and 0.68-214 endotoxin units per cubic meter of air (EU/m3), respectively. Endotoxins were characterized with the skewed distribution (arithmetic mean (AM) = 38.8 EU/m3, geometric mean (GM) = 15.4 EU/m3, geometric standard deviation (GSD) = 4.21). Through the use of multifactor analysis, which excluded the main confounders (inhalable dust and smoking habit) it was found that, despite low levels of endotoxin, it had significant impact on the observed across-shift decline in FEV1 (p = 0.044). For this parameter, the regression slope was additionally calculated (r = -0.017, p = 0.071). Relatively low levels of endotoxin among sewage treatment plant workers may cause small, but significant across-shift declines in FEV1. The observed relationship was independent of organic dust concentrations and smoking habit. The respiratory protection should be provided for STPW. This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL license.

Mutation of the OsSAC1 Gene, which Encodes an Endoplasmic Reticulum Protein with an Unknown Function, Causes Sugar Accumulation in Rice Leaves.

PubMed

Zhu, Xiaoyan; Shen, Wenqiang; Huang, Junyang; Zhang, Tianquan; Zhang, Xiaobo; Cui, Yuanjiang; Sang, Xianchun; Ling, Yinghua; Li, Yunfeng; Wang, Nan; Zhao, Fangmin; Zhang, Changwei; Yang, Zhenglin; He, Guanghua

2018-03-01

Sugars are the most abundant organic compounds produced by plants, and can be used to build carbon skeletons and generate energy. The sugar accumulation 1 (OsSAC1) gene encodes a protein with an unknown function that exhibits four N-terminal transmembrane regions and two conserved domains of unknown function, DUF4220 and DUF594. OsSAC1 was found to be poorly and specifically expressed at the bottoms of young leaves and in the developing leaf sheaths. Subcellular location results showed that OsSAC1 was co-localized with ER:mCherry and targeted the endoplasmic reticulum (ER). OsSAC1 has been found to affect sugar partitioning in rice (Oryza sativa). I2/KI starch staining, ultrastructure observations and starch content measurements indicated that more and larger starch granules accumulated in ossac1 source leaves than in wild-type (WT) source leaves. Additionally, higher sucrose and glucose concentrations accumulated in the ossac1 source leaves than in WT source leaves, whereas lower sucrose and glucose concentrations were observed in the ossac1 young leaves and developing leaf sheaths than in those of the WT. Much greater expression of OsAGPL1 and OsAGPS1 (responsible for starch synthesis) and significantly less expression of OscFBP1, OscFBP2, OsSPS1 and OsSPS11 (responsible for sucrose synthesis) and OsSWEET11, OsSWEET14 and OsSUT1 (responsible for sucrose loading) occurred in ossac1 source leaves than in WT source leaves. A greater amount of the rice plasmodesmatal negative regulator OsGSD1 was detected in ossac1 young leaves and developing leaf sheaths than in those of the WT. These results suggest that ER-targeted OsSAC1 may indirectly regulate sugar partitioning in carbon-demanding young leaves and developing leaf sheaths.

Validation of a Telephone-administered Geriatric Depression Scale in a Hispanic Elderly Population

PubMed Central

Carrete, Paula; Augustovski, Federico; Gimpel, Nora; Fernandez, Sebastian; Di Paolo, Rodolfo; Schaffer, Irene; Rubinstein, Fernando

2001-01-01

OBJECTIVE To develop and validate a Spanish version of the Geriatric Depression Scale (GDS) for telephone administration. DESIGN, SETTING, AND PATIENTS The original version of the GDS was translated into Spanish. A random sample of 282 ambulatory elderly individuals was contacted by phone. Those completing the phone GDS (GDS-T) were asked to schedule an appointment within two weeks in which we collected data on demographics, physical exam, functional and mental status, and a face-to-face version of the GDS (GDS-P). We estimated question-to-question κ statistics and the Pearson correlation coefficient between the GDS-T and GDS-P scores. We evaluated reliability of the GDS-T and GDS-P using the Cronbach's α coefficient. We estimated the sensitivity, specificity, and criterion validity of the GDS using the DSM IV criteria for depression as our gold standard. RESULTS Thirty patients (11%) refused to participate. Of the remaining 252 patients, 169 (67%) attended the personal interview. The Cronbach's α coefficient was 0.85 for GSD-P and 0.88 for GDS-T. Sensitivity and specificity were 88% and 82% for GDS-P and 84% and 79% for GDS-T. The prevalence of depression in the group completing both scales was 12.8% using the GDS-P and 14.9% using the GDS-T (P >.05). Among those who only completed the GDS-T, the prevalence was 22.7% (P <.05) suggesting that depressed patients kept their appointments less frequently. CONCLUSIONS The telephone GDS had high internal consistency and was highly correlated with the validated personal administration of the scale, suggesting that it could be a valid instrument for screening of depression among elderly ambulatory Spanish-speaking patients. Because the depression rate was significantly higher among those not presenting to the personal evaluation, the adoption of GDS-T may help detect and plan early interventions in patients who otherwise would not be identified. PMID:11520381

Land Boundary Delineation to Supporting of Program Systematic Complete Land Registration (PTSL) Using Multicopter-RTF Data (Case study: Wotan Village, Panceng Sub District, Gresik district)

NASA Astrophysics Data System (ADS)

Cahyono, A. B.; Deviantari, U. W.

2017-12-01

According to statutory regulation issued by Ministry of Land and Spatial Planning/Head of National Land Agency (BPN) number 35/2016, Comprehensive Systematic land registration is a sequential activity of which continuously and systematically carried out by the government ranging from collecting, processing, recording and presenting, as well as maintaining the physical and juridical data in the form of map and list of land-plots and flats, including the transfer of legal title for land plots and flats with their inherent rights. Delineation is one method to identify land plots by utilizing map image or high resolution photo and defining the boundaries by drawing lines to determine the valid and recognizable boundaries. A guideline to delineate the unregistered land plots may be determined from this two methods’ accuracy result, using general boundary applied to aerial photo taken by multicopter RTF. Data taken from a height of 70 meter on an area obtained a number of 156 photos with 5 GCP resulting in an photo map with GSD 2.14 cm. The 11 samples parcels are selected in the sites of ± 7 ha. There are 11 samples of land parcels are tested. The area difference test for every parcel using a average standard deviation of 17,043 indicates that there are three land parcels which have significant area difference and 8 others do not have significant area difference. Based on the tolerance of National Land Agency, among 11 parcels studied, there are 8 parcels that fullfill the tolerances and three others do not fullfill tolerances. The percentage of area difference average between land registration map and orthophoto is 4,72%. The result shows that the differences in boundaries and areas that may be caused by a systematic error of method in describing the boundaries of the ground.

Respiratory protection from isocyanate exposure in the autobody repair and refinishing industry.

PubMed

Liu, Youcheng; Stowe, Meredith H; Bello, Dhimiter; Woskie, Susan R; Sparer, Judy; Gore, Rebecca; Youngs, Fred; Cullen, Mark R; Redlich, Carrie A

2006-05-01

This study, part of the Survey of Painters and Repairers of Auto bodies by Yale (SPRAY), evaluated the effectiveness of respiratory protection against exposure to aliphatic polyisocyanates. A total of 36 shops were assessed for respiratory protection program completeness; 142 workers were measured for respirator fit factor (FF) using PortaCount Plus respirator fit tester. Twenty-two painters from 21 shops were sampled using NIOSH method 5525 to determine the workplace protection factor (WPF) of negative pressure, air-purifying half-facepiece respirators equipped with organic vapor cartridges and paint prefilters during spray-painting and priming activities. Only 11 shops (30%) had written respiratory protection programs. Eighty percent of all fit tested workers passed the test on the first try with FF >or= 100, and 92% passed the second test after respirator use training. Overall geometric mean (GM) FF was 1012 for all fit tested workers. Significant differences on pass rate (92% vs. 72%) and on FF (1990 vs. 736) were found between previously fit tested workers vs. nontested workers. Twenty-nine WPF samples were collected. The outside facepiece GM concentration of total isocyanate group (NCO) was 378.4 micro g NCO/m(3) with 96% concentrations exceeding the U.K. short-term exposure limit, 70 micro g NCO/m(3), but no in-facepiece concentrations exceeded the limit. The GM WPF of total NCO was 319 (GSD 4) and the 5th percentile was 54. WPF of total NCO was positively correlated with the duration of painting task. FF positively correlated with WPF when FF was 450. We conclude that negative pressure, air-purifying half-facepiece respirators equipped with organic vapor cartridges and paint prefilters provide effective protection against isocyanate exposure in spray and priming operations if workers are properly trained and fitted.

Automated Verification of Spatial Resolution in Remotely Sensed Imagery

NASA Technical Reports Server (NTRS)

Davis, Bruce; Ryan, Robert; Holekamp, Kara; Vaughn, Ronald

2011-01-01

Image spatial resolution characteristics can vary widely among sources. In the case of aerial-based imaging systems, the image spatial resolution characteristics can even vary between acquisitions. In these systems, aircraft altitude, speed, and sensor look angle all affect image spatial resolution. Image spatial resolution needs to be verified with estimators that include the ground sample distance (GSD), the modulation transfer function (MTF), and the relative edge response (RER), all of which are key components of image quality, along with signal-to-noise ratio (SNR) and dynamic range. Knowledge of spatial resolution parameters is important to determine if features of interest are distinguishable in imagery or associated products, and to develop image restoration algorithms. An automated Spatial Resolution Verification Tool (SRVT) was developed to rapidly determine the spatial resolution characteristics of remotely sensed aerial and satellite imagery. Most current methods for assessing spatial resolution characteristics of imagery rely on pre-deployed engineered targets and are performed only at selected times within preselected scenes. The SRVT addresses these insufficiencies by finding uniform, high-contrast edges from urban scenes and then using these edges to determine standard estimators of spatial resolution, such as the MTF and the RER. The SRVT was developed using the MATLAB programming language and environment. This automated software algorithm assesses every image in an acquired data set, using edges found within each image, and in many cases eliminating the need for dedicated edge targets. The SRVT automatically identifies high-contrast, uniform edges and calculates the MTF and RER of each image, and when possible, within sections of an image, so that the variation of spatial resolution characteristics across the image can be analyzed. The automated algorithm is capable of quickly verifying the spatial resolution quality of all images within a data set, enabling the appropriate use of those images in a number of applications.

Mapping snow depth in alpine terrain with remotely piloted aerial systems and structure-from-motion photogrammetry - first results from a pilot study

NASA Astrophysics Data System (ADS)

Adams, Marc; Fromm, Reinhard; Bühler, Yves; Bösch, Ruedi; Ginzler, Christian

2016-04-01

Detailed information on the spatio-temporal distribution of seasonal snow in the alpine terrain plays a major role for the hydrological cycle, natural hazard management, flora and fauna, as well as tourism. Current methods are mostly only valid on a regional scale or require a trade-off between the data's availability, cost and resolution. During a one-year pilot study, we investigated the potential of remotely piloted aerial systems (RPAS) and structure-from-motion photogrammetry for snow depth mapping. We employed multi-copter and fixed-wing RPAS, equipped with different low-cost, off-the shelf sensors, at four test sites in Austria and Switzerland. Over 30 flights were performed during the winter 2014/15, where different camera settings, filters and lenses, as well as data collection routines were tested. Orthophotos and digital surface models (DSM) where calculated from the imagery using structure-from-motion photogrammetry software. Snow height was derived by subtracting snow-free from snow-covered DSMs. The RPAS-results were validated against data collected using a variety of well-established remote sensing (i.e. terrestrial laser scanning, large frame aerial sensors) and in-situ measurement techniques. The results show, that RPAS i) are able to map snow depth within accuracies of 0.07-0.15 m root mean square error (RMSE), when compared to traditional in-situ data; ii) can be operated at lower cost, easier repeatability, less operational constraints and higher GSD than large frame aerial sensors on-board manned aircraft, while achieving significantly higher accuracies; iii) are able to acquire meaningful data even under harsh environmental conditions above 2000 m a.s.l. (turbulence, low temperature and high irradiance, low air density). While providing a first prove-of-concept, the study also showed future challenges and limitations of RPAS-based snow depth mapping, including a high dependency on correct co-registration of snow-free and snow-covered height measurements, as well as a significant impact of the underlying vegetation and illumination of the snow surface on the fidelity of the results.

The Experimental Regional Ensemble Forecast System (ExREF): Its Use in NWS Forecast Operations and Preliminary Verification

NASA Technical Reports Server (NTRS)

Reynolds, David; Rasch, William; Kozlowski, Daniel; Burks, Jason; Zavodsky, Bradley; Bernardet, Ligia; Jankov, Isidora; Albers, Steve

2014-01-01

The Experimental Regional Ensemble Forecast (ExREF) system is a tool for the development and testing of new Numerical Weather Prediction (NWP) methodologies. ExREF is run in near-realtime by the Global Systems Division (GSD) of the NOAA Earth System Research Laboratory (ESRL) and its products are made available through a website, an ftp site, and via the Unidata Local Data Manager (LDM). The ExREF domain covers most of North America and has 9-km horizontal grid spacing. The ensemble has eight members, all employing WRF-ARW. The ensemble uses a variety of initial conditions from LAPS and the Global Forecasting System (GFS) and multiple boundary conditions from the GFS ensemble. Additionally, a diversity of physical parameterizations is used to increase ensemble spread and to account for the uncertainty in forecasting extreme precipitation events. ExREF has been a component of the Hydrometeorology Testbed (HMT) NWP suite in the 2012-2013 and 2013-2014 winters. A smaller domain covering just the West Coast was created to minimize band-width consumption for the NWS. This smaller domain has and is being distributed to the National Weather Service (NWS) Weather Forecast Office and California Nevada River Forecast Center in Sacramento, California, where it is ingested into the Advanced Weather Interactive Processing System (AWIPS I and II) to provide guidance on the forecasting of extreme precipitation events. This paper will review the cooperative effort employed by NOAA ESRL, NASA SPoRT (Short-term Prediction Research and Transition Center), and the NWS to facilitate the ingest and display of ExREF data utilizing the AWIPS I and II D2D and GFE (Graphical Software Editor) software. Within GFE is a very useful verification software package called BoiVer that allows the NWS to utilize the River Forecast Center's 4 km gridded QPE to compare with all operational NWP models 6-hr QPF along with the ExREF mean 6-hr QPF so the forecasters can build confidence in the use of the ExREF in preparing their rainfall forecasts. Preliminary results will be presented.

Asphalt fume exposure levels in North American asphalt production and roofing manufacturing operations.

PubMed

Axten, Charles W; Fayerweather, William E; Trumbore, David C; Mueller, Dennis J; Sampson, Arthur F

2012-01-01

This study extends by 8 years (1998-2005) a previous survey of asphalt fume exposures within North American asphalt processing and roofing product manufacturing workers. It focuses on characterizing personal, full-shift samples and seeks to address several limitations of the previous survey. Five major roofing manufacturers with established occupational health programs submitted workplace asphalt fume sampling results to a central repository for review and analysis. A certified industrial hygienist-led quality assurance team oversaw the data collection, consolidation, and analysis efforts. The analysis dataset consisted of 1261 personal exposure samples analyzed for total particulate (TP) and benzene soluble fraction (BSF) using existing NIOSH methods. For BSF, the survey's arithmetic (0.25 mg/m(3), SD = 0.62) and geometric (0.12 mg/m(3), GSD = 2.88) means indicate that the industry has sustained the control levels achieved in the late 1980s, early 1990s. Similar results were found for TP. The survey-wide summary statistics are consistent with other post-1990 multi-company exposure studies. Although these findings indicate that currently available controls are capable of achieving substantial (95%) compliance with the current threshold limit value in asphalt processing and inorganic shingle and roll plants, they also show that the majority of plants are not achieving this level of exposure control, and that exposures are significantly higher in plants making other product lines, particularly organic felt products. The current retrospective survey of existing company exposure data, like its predecessor, has several important limitations. These include lack of data on smaller manufacturers and on several commercially important product lines; insufficient information on the prevalence and effectiveness of engineering controls; no standard criteria by which to define and assess exposures in non-routine operations; and a paucity of exposure data collected as part of a random sampling strategy. To improve efforts to characterize exposures and potential health risks in roofing plants, a prospective program is currently being developed and piloted with the aim of building a more complete, higher-quality database based on a common industrial hygiene protocol.

Polymorphisms in the Tlr4 and Tlr5 Gene Are Significantly Associated with Inflammatory Bowel Disease in German Shepherd Dogs

PubMed Central

Kathrani, Aarti; House, Arthur; Catchpole, Brian; Murphy, Angela; German, Alex; Werling, Dirk; Allenspach, Karin

2010-01-01

Inflammatory bowel disease (IBD) is considered to be the most common cause of vomiting and diarrhoea in dogs, and the German shepherd dog (GSD) is particularly susceptible. The exact aetiology of IBD is unknown, however associations have been identified between specific single-nucleotide polymorphisms (SNPs) in Toll-like receptors (TLRs) and human IBD. However, to date, no genetic studies have been undertaken in canine IBD. The aim of this study was to investigate whether polymorphisms in canine TLR 2, 4 and 5 genes are associated with IBD in GSDs. Mutational analysis of TLR2, TLR4 and TLR5 was performed in 10 unrelated GSDs with IBD. Four non-synonymous SNPs (T23C, G1039A, A1571T and G1807A) were identified in the TLR4 gene, and three non-synonymous SNPs (G22A, C100T and T1844C) were identified in the TLR5 gene. The non-synonymous SNPs identified in TLR4 and TLR5 were evaluated further in a case-control study using a SNaPSHOT multiplex reaction. Sequencing information from 55 unrelated GSDs with IBD were compared to a control group consisting of 61 unrelated GSDs. The G22A SNP in TLR5 was significantly associated with IBD in GSDs, whereas the remaining two SNPs were found to be significantly protective for IBD. Furthermore, the two SNPs in TLR4 (A1571T and G1807A) were in complete linkage disequilibrium, and were also significantly associated with IBD. The TLR5 risk haplotype (ACC) without the two associated TLR4 SNP alleles was significantly associated with IBD, however the presence of the two TLR4 SNP risk alleles without the TLR5 risk haplotype was not statistically associated with IBD. Our study suggests that the three TLR5 SNPs and two TLR4 SNPs; A1571T and G1807A could play a role in the pathogenesis of IBD in GSDs. Further studies are required to confirm the functional importance of these polymorphisms in the pathogenesis of this disease. PMID:21203467

Polymorphisms in the TLR4 and TLR5 gene are significantly associated with inflammatory bowel disease in German shepherd dogs.

PubMed

Kathrani, Aarti; House, Arthur; Catchpole, Brian; Murphy, Angela; German, Alex; Werling, Dirk; Allenspach, Karin

2010-12-23

Inflammatory bowel disease (IBD) is considered to be the most common cause of vomiting and diarrhoea in dogs, and the German shepherd dog (GSD) is particularly susceptible. The exact aetiology of IBD is unknown, however associations have been identified between specific single-nucleotide polymorphisms (SNPs) in Toll-like receptors (TLRs) and human IBD. However, to date, no genetic studies have been undertaken in canine IBD. The aim of this study was to investigate whether polymorphisms in canine TLR 2, 4 and 5 genes are associated with IBD in GSDs. Mutational analysis of TLR2, TLR4 and TLR5 was performed in 10 unrelated GSDs with IBD. Four non-synonymous SNPs (T23C, G1039A, A1571T and G1807A) were identified in the TLR4 gene, and three non-synonymous SNPs (G22A, C100T and T1844C) were identified in the TLR5 gene. The non-synonymous SNPs identified in TLR4 and TLR5 were evaluated further in a case-control study using a SNaPSHOT multiplex reaction. Sequencing information from 55 unrelated GSDs with IBD were compared to a control group consisting of 61 unrelated GSDs. The G22A SNP in TLR5 was significantly associated with IBD in GSDs, whereas the remaining two SNPs were found to be significantly protective for IBD. Furthermore, the two SNPs in TLR4 (A1571T and G1807A) were in complete linkage disequilibrium, and were also significantly associated with IBD. The TLR5 risk haplotype (ACC) without the two associated TLR4 SNP alleles was significantly associated with IBD, however the presence of the two TLR4 SNP risk alleles without the TLR5 risk haplotype was not statistically associated with IBD. Our study suggests that the three TLR5 SNPs and two TLR4 SNPs; A1571T and G1807A could play a role in the pathogenesis of IBD in GSDs. Further studies are required to confirm the functional importance of these polymorphisms in the pathogenesis of this disease.

Dust exposure and chronic respiratory symptoms among coffee curing workers in Kilimanjaro: a cross sectional study.

PubMed

Sakwari, Gloria; Bråtveit, Magne; Mamuya, Simon H D; Moen, Bente E

2011-11-24

Coffee processing causes organic dust exposure which may lead to development of respiratory symptoms. Previous studies have mainly focused on workers involved in roasting coffee in importing countries. This study was carried out to determine total dust exposure and respiratory health of workers in Tanzanian primary coffee-processing factories. A cross sectional study was conducted among 79 workers in two coffee factories, and among 73 control workers in a beverage factory. Personal samples of total dust (n = 45 from the coffee factories and n = 19 from the control factory) were collected throughout the working shift from the breathing zone of the workers. A questionnaire with modified questions from the American Thoracic Society questionnaire was used to assess chronic respiratory symptoms. Differences between groups were tested by using independent t-tests and Chi square tests. Poisson Regression Model was used to estimate prevalence ratio, adjusting for age, smoking, presence of previous lung diseases and years worked in dusty factories. All participants were male. The coffee workers had a mean age of 40 years and were older than the controls (31 years). Personal total dust exposure in the coffee factories were significantly higher than in the control factory (geometric mean (GM) 1.23 mg/m3, geometric standard deviation (GSD) (0.8) vs. 0.21(2.4) mg/m3). Coffee workers had significantly higher prevalence than controls for cough with sputum (23% vs. 10%; Prevalence ratio (PR); 2.5, 95% CI 1.0-5.9) and chest tightness (27% vs. 13%; PR; 2.4, 95% CI 1.1-5.2). The prevalence of morning cough, cough with and without sputum for 4 days or more in a week was also higher among coffee workers than among controls. However, these differences were not statistically significant. Workers exposed to coffee dust reported more respiratory symptoms than did the controls. This might relate to their exposure to coffee dust. Interventions for reduction of dust levels and provision of respiratory protective equipment are recommended.

Urinary excretion of unmetabolized benzene as an indicator of benzene exposure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ghittori, S.; Fiorentino, M.L.; Maestri, L.

1993-03-01

Benzene concentrations in urine samples (Cu, ng/L) from 110 workers exposed to benzene in chemical plants and gasoline pumps were determined by injecting urine supernate into a gas chromatograph. The urine was saturated with anhydrous N2SO4 to facilitate the passage of benzene in the air over the urine. The solvent was stripped from the urine surface and concentrated on an adsorbent substrate (Carbotrap tube) by means of a suction pump (flow rate 150 ml/m). Wash-up of the head space was achieved by simultaneous intake of filtered air through charcoal. Benzene was thermically desorbed and injected in a column (thermal tubemore » disorder, Supelco; 370 degrees C thermal flash; borosilicate capillary glass column SPB-1, 60 m length, 0.75 mm ID, 1 microns film thickness; GC Dani 8580-FID). Benzene concentrations in the urine from 40 non-exposed subjects (20 smokers > 20 cigarette/d and 20 nonsmokers) were also determined [median value of 790 ng/L (10.17 nmol/L) and 131 ng/L (1.70 nmol/L), respectively]. The 8-h time-weighted exposure intensity (Cl, micrograms/m3) of individual workers was monitored by means of charcoal tubes. The median value for exposure to benzene was 736 micrograms/m3 (9.42 mumol/m3) [geometric standard deviation (GSD) = 2.99; range 64 micrograms/m3 (0.82 mumol/m3) to 13,387 micrograms/m3 (171.30 mumol/m3)]. The following linear correlation was found between benzene concentrations in urine (Cu, ng/L) and benzene concentrations in the breathing zone (Cl, micrograms/m3): log(Cu) = 0.645 x log(Cl) + 1.399 r = .559, n = 110, p < .0001 With exclusion of workers who smoked from the study, the correlation between air benzene concentration and benzene measured in urine was: log(Cu) = 0.872 x log(Cl) + 0.6 r = .763, n = 63, p < .0001 The study results indicate that the urinary level of benzene is an indicator of occupational exposure to benzene.« less

Development of a new test system to determine penetration of multi-walled carbon nanotubes through filtering facepiece respirators

PubMed Central

Vo, Evanly; Zhuang, Ziqing

2015-01-01

Carbon nanotubes (CNTs) are currently used in numerous industrial and biomedical applications. Recent studies suggest that workers may be at risk of adverse health effects if they are exposed to CNTs. A National Institute for Occupational Safety and Health (NIOSH) survey of the carbonaceous nanomaterial industry found that 77% of the companies used respiratory protection. Elastomeric half-mask respirators and filtering facepiece respirators (FFRs) are commonly used. Although numerous respirator filtration studies have been done with surrogate engineered nanoparticles, such as sodium chloride, penetration data from engineered nanoparticles such as CNTs are lacking. The aims of this study were to develop a new CNT aerosol respirator testing system and to determine multi-walled CNT (MWCNT) penetration through FFRs. A custom-designed CNT aerosol respirator testing system (CNT-ARTS) was developed which was capable of producing a sufficient amount of airborne MWCNTs for testing of high efficiency FFRs. The size distribution of airborne MWCNTs was 20–10,000 nm, with 99% of the particles between 25 and 2840 nm. The count median diameter (CMD) was 209 nm with a geometric standard deviation (GSD) of 1.98. This particle size range is similar to those found in some work environments (particles ≤6000 nm). The penetration of MWCNTs through six tested FFR models at two constant flow rates of 30 and 85 LPM was determined. Penetration at 85 LPM (0.58–2.04% for N95, 0.15–0.32% for N99, and 0.007–0.009% for P100 FFRs) was greater compared with the values at 30 LPM (0.28–1.79% for N95, 0.10–0.24% for N99, and 0.005–0.006% for P100 FFRs). The most penetrating particle size through all six tested FFR models was found to be in the range of 25–130 nm and 35–200 nm for the 30-LPM and 85-LPM flow rates, respectively. PMID:26166842

Investigation of Aerosol Surface Area Estimation from Number and Mass Concentration Measurements: Particle Density Effect

PubMed Central

Ku, Bon Ki; Evans, Douglas E.

2015-01-01

For nanoparticles with nonspherical morphologies, e.g., open agglomerates or fibrous particles, it is expected that the actual density of agglomerates may be significantly different from the bulk material density. It is further expected that using the material density may upset the relationship between surface area and mass when a method for estimating aerosol surface area from number and mass concentrations (referred to as “Maynard’s estimation method”) is used. Therefore, it is necessary to quantitatively investigate how much the Maynard’s estimation method depends on particle morphology and density. In this study, aerosol surface area estimated from number and mass concentration measurements was evaluated and compared with values from two reference methods: a method proposed by Lall and Friedlander for agglomerates and a mobility based method for compact nonspherical particles using well-defined polydisperse aerosols with known particle densities. Polydisperse silver aerosol particles were generated by an aerosol generation facility. Generated aerosols had a range of morphologies, count median diameters (CMD) between 25 and 50 nm, and geometric standard deviations (GSD) between 1.5 and 1.8. The surface area estimates from number and mass concentration measurements correlated well with the two reference values when gravimetric mass was used. The aerosol surface area estimates from the Maynard’s estimation method were comparable to the reference method for all particle morphologies within the surface area ratios of 3.31 and 0.19 for assumed GSDs 1.5 and 1.8, respectively, when the bulk material density of silver was used. The difference between the Maynard’s estimation method and surface area measured by the reference method for fractal-like agglomerates decreased from 79% to 23% when the measured effective particle density was used, while the difference for nearly spherical particles decreased from 30% to 24%. The results indicate that the use of particle density of agglomerates improves the accuracy of the Maynard’s estimation method and that an effective density should be taken into account, when known, when estimating aerosol surface area of nonspherical aerosol such as open agglomerates and fibrous particles. PMID:26526560

Dust exposure and chronic respiratory symptoms among coffee curing workers in Kilimanjaro: a cross sectional study

PubMed Central

2011-01-01

Background Coffee processing causes organic dust exposure which may lead to development of respiratory symptoms. Previous studies have mainly focused on workers involved in roasting coffee in importing countries. This study was carried out to determine total dust exposure and respiratory health of workers in Tanzanian primary coffee-processing factories. Methods A cross sectional study was conducted among 79 workers in two coffee factories, and among 73 control workers in a beverage factory. Personal samples of total dust (n = 45 from the coffee factories and n = 19 from the control factory) were collected throughout the working shift from the breathing zone of the workers. A questionnaire with modified questions from the American Thoracic Society questionnaire was used to assess chronic respiratory symptoms. Differences between groups were tested by using independent t-tests and Chi square tests. Poisson Regression Model was used to estimate prevalence ratio, adjusting for age, smoking, presence of previous lung diseases and years worked in dusty factories. Results All participants were male. The coffee workers had a mean age of 40 years and were older than the controls (31 years). Personal total dust exposure in the coffee factories were significantly higher than in the control factory (geometric mean (GM) 1.23 mg/m3, geometric standard deviation (GSD) (0.8) vs. 0.21(2.4) mg/m3). Coffee workers had significantly higher prevalence than controls for cough with sputum (23% vs. 10%; Prevalence ratio (PR); 2.5, 95% CI 1.0 - 5.9) and chest tightness (27% vs. 13%; PR; 2.4, 95% CI 1.1 - 5.2). The prevalence of morning cough, cough with and without sputum for 4 days or more in a week was also higher among coffee workers than among controls. However, these differences were not statistically significant. Conclusion Workers exposed to coffee dust reported more respiratory symptoms than did the controls. This might relate to their exposure to coffee dust. Interventions for reduction of dust levels and provision of respiratory protective equipment are recommended. PMID:22114929

Aerodynamic particle size analysis of aerosols from pressurized metered-dose inhalers: comparison of Andersen 8-stage cascade impactor, next generation pharmaceutical impactor, and model 3321 Aerodynamic Particle Sizer aerosol spectrometer.

PubMed

Mitchell, Jolyon P; Nagel, Mark W; Wiersema, Kimberly J; Doyle, Cathy C

2003-10-22

The purpose of this research was to compare three different methods for the aerodynamic assessment of (1) chloroflurocarbon (CFC)--fluticasone propionate (Flovent), (2) CFC-sodium cromoglycate (Intal), and (3) hydrofluoroalkane (HFA)--beclomethasone dipropionate (Qvar) delivered by pressurized metered dose inhaler. Particle size distributions were compared determining mass median aerodynamic diameter (MMAD), geometric standard deviation (GSD), and fine particle fraction <4.7 microm aerodynamic diameter (FPF(<4.7 microm)). Next Generation Pharmaceutical Impactor (NGI)-size distributions for Flovent comprised finer particles than determined by Andersen 8-stage impactor (ACI) (MMAD = 2.0 +/- 0.05 micro m [NGI]; 2.8 +/- 0.07 microm [ACI]); however, FPF(<4.7 microm) by both impactors was in the narrow range 88% to 93%. Size distribution agreement for Intal was better (MMAD = 4.3 +/- 0.19 microm (NGI), 4.2 +/- 0.13 microm (ACI), with FPF(<4.7 microm) ranging from 52% to 60%. The Aerodynamic Particle Sizer (APS) undersized aerosols produced with either formulation (MMAD = 1.8 +/- 0.07 micro m and 3.2 +/- 0.02 micro m for Flovent and Intal, respectively), but values of FPF(<4.7 microm)from the single-stage impactor (SSI) located at the inlet to the APS (82.9% +/- 2.1% [Flovent], 46.4% +/- 2.4% [Intal]) were fairly close to corresponding data from the multi-stage impactors. APS-measured size distributions for Qvar (MMAD = 1.0 +/- 0.03 micro m; FPF(<4.7 micro m)= 96.4% +/- 2.5%), were in fair agreement with both NGI (MMAD = 0.9 +/- 0.03 micro m; FPF(<4.7 microm)= 96.7% +/- 0.7%), and ACI (MMAD = 1.2 +/- 0.02 microm, FPF(<4.7 microm)= 98% +/- 0.5%), but FPF(<4.7 microm) from the SSI (67.1% +/- 4.1%) was lower than expected, based on equivalent data obtained by the other techniques. Particle bounce, incomplete evaporation of volatile constituents and the presence of surfactant particles are factors that may be responsible for discrepancies between the techniques.

Placental Pathology Associated with Household Air Pollution in a Cohort of Pregnant Women from Dar es Salaam, Tanzania.

PubMed

Wylie, Blair J; Matechi, Emmanuel; Kishashu, Yahya; Fawzi, Wafaie; Premji, Zul; Coull, Brent A; Hauser, Russ; Ezzati, Majid; Roberts, Drucilla J

2017-01-01

Smoke from the burning of biomass fuels has been linked with adverse pregnancy outcomes such as low birth weight, stillbirth, and prematurity. To identify potential underlying mechanisms of adverse perinatal outcomes, we explored the association of placental pathology with household air pollution in pregnant women from urban/periurban Tanzania who cook predominantly with charcoal. Between 2011 and 2013, we measured personal exposures to fine particulate matter (PM2.5) and carbon monoxide (CO) over 72 hr among a cohort of Tanzanian pregnant women. Placentas were collected after delivery for examination. Placental pathologies of inflammatory, hypoxic, ischemic/hypertensive, infectious and thrombotic etiologies were diagnosed, blinded to exposure levels. Using multiple logistic regression, we explored the association of PM2.5 and CO exposure with placental pathology. One hundred sixteen women had personal air exposure measurements and placental histopathology available for analysis. PM2.5 and CO exposures were moderate [geometric means (GSD) were 40.5 μg/m3 (17.3) and 2.21 ppm (1.47) respectively]; 88.6% of PM2.5 measurements exceeded World Health Organization air quality guidelines. We observed an increase in the odds (per 1-unit increase in exposure on the ln-scale) of fetal thrombotic vasculopathy (FTV) both with increasing PM2.5 [adjusted odds ratio (aOR) = 5.5; 95% CI: 1.1, 26.8] and CO measurements (aOR = 2.5; 95% CI: 1.0, 6.4) in adjusted models only. FTV also was more common among pregnancies complicated by stillbirth or low birth weight. Fetal thrombosis may contribute to the adverse outcomes associated with household air pollution from cook stoves during pregnancy. Larger studies are necessary for confirmation. Citation: Wylie BJ, Matechi E, Kishashu Y, Fawzi W, Premji Z, Coull BA, Hauser R, Ezzati M, Roberts D. 2017. Placental pathology associated with household air pollution in a cohort of pregnant women from Dar es Salaam, Tanzania. Environ Health Perspect 125:134-140; http://dx.doi.org/10.1289/EHP256.

Investigation of Aerosol Surface Area Estimation from Number and Mass Concentration Measurements: Particle Density Effect.

PubMed

Ku, Bon Ki; Evans, Douglas E

2012-04-01

For nanoparticles with nonspherical morphologies, e.g., open agglomerates or fibrous particles, it is expected that the actual density of agglomerates may be significantly different from the bulk material density. It is further expected that using the material density may upset the relationship between surface area and mass when a method for estimating aerosol surface area from number and mass concentrations (referred to as "Maynard's estimation method") is used. Therefore, it is necessary to quantitatively investigate how much the Maynard's estimation method depends on particle morphology and density. In this study, aerosol surface area estimated from number and mass concentration measurements was evaluated and compared with values from two reference methods: a method proposed by Lall and Friedlander for agglomerates and a mobility based method for compact nonspherical particles using well-defined polydisperse aerosols with known particle densities. Polydisperse silver aerosol particles were generated by an aerosol generation facility. Generated aerosols had a range of morphologies, count median diameters (CMD) between 25 and 50 nm, and geometric standard deviations (GSD) between 1.5 and 1.8. The surface area estimates from number and mass concentration measurements correlated well with the two reference values when gravimetric mass was used. The aerosol surface area estimates from the Maynard's estimation method were comparable to the reference method for all particle morphologies within the surface area ratios of 3.31 and 0.19 for assumed GSDs 1.5 and 1.8, respectively, when the bulk material density of silver was used. The difference between the Maynard's estimation method and surface area measured by the reference method for fractal-like agglomerates decreased from 79% to 23% when the measured effective particle density was used, while the difference for nearly spherical particles decreased from 30% to 24%. The results indicate that the use of particle density of agglomerates improves the accuracy of the Maynard's estimation method and that an effective density should be taken into account, when known, when estimating aerosol surface area of nonspherical aerosol such as open agglomerates and fibrous particles.

Potential of VIIRS Time Series Data for Aiding the USDA Forest Service Early Warning System for Forest Health Threats: A Gypsy Moth Defoliation Case Study

NASA Technical Reports Server (NTRS)

Spruce, Joseph P.; Ryan, Robert E.; Smoot, James; Kuper, Phillip; Prados, Donald; Russell, Jeffrey; Ross, Kenton; Gasser, Gerald; Sader, Steven; McKellip, Rodney

2007-01-01

This report details one of three experiments performed during FY 2007 for the NASA RPC (Rapid Prototyping Capability) at Stennis Space Center. This RPC experiment assesses the potential of VIIRS (Visible/Infrared Imager/Radiometer Suite) and MODIS (Moderate Resolution Imaging Spectroradiometer) data for detecting and monitoring forest defoliation from the non-native Eurasian gypsy moth (Lymantria dispar). The intent of the RPC experiment was to assess the degree to which VIIRS data can provide forest disturbance monitoring information as an input to a forest threat EWS (Early Warning System) as compared to the level of information that can be obtained from MODIS data. The USDA Forest Service (USFS) plans to use MODIS products for generating broad-scaled, regional monitoring products as input to an EWS for forest health threat assessment. NASA SSC is helping the USFS to evaluate and integrate currently available satellite remote sensing technologies and data products for the EWS, including the use of MODIS products for regional monitoring of forest disturbance. Gypsy moth defoliation of the mid-Appalachian highland region was selected as a case study. Gypsy moth is one of eight major forest insect threats listed in the Healthy Forest Restoration Act (HFRA) of 2003; the gypsy moth threatens eastern U.S. hardwood forests, which are also a concern highlighted in the HFRA of 2003. This region was selected for the project because extensive gypsy moth defoliation occurred there over multiple years during the MODIS operational period. This RPC experiment is relevant to several nationally important mapping applications, including agricultural efficiency, coastal management, ecological forecasting, disaster management, and carbon management. In this experiment, MODIS data and VIIRS data simulated from MODIS were assessed for their ability to contribute broad, regional geospatial information on gypsy moth defoliation. Landsat and ASTER (Advanced Spaceborne Thermal Emission and Reflection Radiometer) data were used to assess the quality of gypsy moth defoliation mapping products derived from MODIS data and from simulated VIIRS data. The project focused on use of data from MODIS Terra as opposed to MODIS Aqua mainly because only MODIS Terra data was collected during 2000 and 2001-years with comparatively high amounts of gypsy moth defoliation within the study area. The project assessed the quality of VIIRS data simulation products. Hyperion data was employed to assess the quality of MODIS-based VIIRS simulation datasets using image correlation analysis techniques. The ART (Application Research Toolbox) software was used for data simulation. Correlation analysis between MODIS-simulated VIIRS data and Hyperion-simulated VIIRS data for red, NIR (near-infrared), and NDVI (Normalized Difference Vegetation Index) image data products collectively indicate that useful, effective VIIRS simulations can be produced using Hyperion and MODIS data sources. The r(exp 2) for red, NIR, and NDVI products were 0.56, 0.63, and 0.62, respectively, indicating a moderately high correlation between the 2 data sources. Temporal decorrelation from different data acquisition times and image misregistration may have lowered correlation results. The RPC experiment also generated MODIS-based time series data products using the TSPT (Time Series Product Tool) software. Time series of simulated VIIRS NDVI products were produced at approximately 400-meter resolution GSD (Ground Sampling Distance) at nadir for comparison to MODIS NDVI products at either 250- or 500-meter GSD. The project also computed MODIS (MOD02) NDMI (Normalized Difference Moisture Index) products at 500-meter GSD for comparison to NDVI-based products. For each year during 2000-2006, MODIS and VIIRS (simulated from MOD02) time series were computed during the peak gypsy moth defoliation time frame in the study area (approximately June 10 through July 27). Gypsy moth defoliation mapping products from simated VIIRS and MOD02 time series were produced using multiple methods, including image classification and change detection via image differencing. The latter enabled an automated defoliation detection product computed using percent change in maximum NDVI for a peak defoliation period during 2001 compared to maximum NDVI across the entire 2000-2006 time frame. Final gypsy moth defoliation mapping products were assessed for accuracy using randomly sampled locations found on available geospatial reference data (Landsat and ASTER data in conjunction with defoliation map data from the USFS). Extensive gypsy moth defoliation patches were evident on screen displays of multitemporal color composites derived from MODIS data and from simulated VIIRS vegetation index data. Such defoliation was particularly evident for 2001, although widespread denuded forests were also seen for 2000 and 2003. These visualizations were validated using aforementioned reference data. Defoliation patches were visible on displays of MODIS-based NDVI and NDMI data. The viewing of apparent defoliation patches on all of these products necessitated adoption of a specialized temporal data processing method (e.g., maximum NDVI during the peak defoliation time frame). The frequency of cloud cover necessitated this approach. Multitemporal simulated VIIRS and MODIS Terra data both produced effective general classifications of defoliated forest versus other land cover. For 2001, the MOD02-simulated VIIRS 400-meter NDVI classification produced a similar yet slightly lower overall accuracy (87.28 percent with 0.72 Kappa) than the MOD02 250-meter NDVI classification (88.44 percent with 0.75 Kappa). The MOD13 250-meter NDVI classification had a lower overall accuracy (79.13 percent) and a much lower Kappa (0.46). The report discusses accuracy assessment results in much more detail, comparing overall classification and individual class accuracy statistics for simulated VIIRS 400-meter NDVI, MOD02 250-meter NDVI, MOD02-500 meter NDVI, MOD13 250-meter NDVI, and MOD02 500-meter NDMI classifications. Automated defoliation detection products from simulated VIIRS and MOD02 data for 2001 also yielded similar, relatively high overall classification accuracy (85.55 percent for the VIIRS 400-meter NDVI versus 87.28 percent for the MOD02 250-meter NDVI). In contrast, the USFS aerial sketch map of gypsy moth defoliation showed a lower overall classification accuracy at 73.64 percent. The overall classification Kappa values were also similar for the VIIRS (approximately 0.67 Kappa) versus the MOD02 (approximately 0.72 Kappa) automated defoliation detection product, which were much higher than the values exhibited by the USFS sketch map product (overall Kappa of approximately 0.47). The report provides additional details on the accuracy of automated gypsy moth defoliation detection products compared with USFS sketch maps. The results suggest that VIIRS data can be effectively simulated from MODIS data and that VIIRS data will produce gypsy moth defoliation mapping products that are similar to MODIS-based products. The results of the RPC experiment indicate that VIIRS and MODIS data products have good potential for integration into the forest threat EWS. The accuracy assessment was performed only for 2001 because of time constraints and a relative scarcity of cloud-free Landsat and ASTER data for the peak defoliation period of the other years in the 2000-2006 time series. Additional work should be performed to assess the accuracy of gypsy moth defoliation detection products for additional years.The study area (mid-Appalachian highlands) and application (gypsy moth forest defoliation) are not necessarily representative of all forested regions and of all forest threat disturbance agents. Additional work should be performed on other inland and coastal regions as well as for other major forest threats.

Topography changes monitoring of small islands using camera drone

NASA Astrophysics Data System (ADS)

Bang, E.

2017-12-01

Drone aerial photogrammetry was conducted for monitoring topography changes of small islands in the east sea of Korea. Severe weather and sea wave is eroding the islands and sometimes cause landslide and falling rock. Due to rugged cliffs in all direction and bad accessibility, ground based survey methods are less efficient in monitoring topography changes of the whole area. Camera drones can provide digital images and movie in every corner of the islands, and drone aerial photogrammetry is powerful to get precise digital surface model (DSM) for a limited area. We have got a set of digital images to construct a textured 3D model of the project area every year since 2014. Flight height is in less than 100m from the top of those islands to get enough ground sampling distance (GSD). Most images were vertically captured with automatic flights, but we also flied drones around the islands with about 30°-45° camera angle for constructing 3D model better. Every digital image has geo-reference, but we set several ground control points (GCPs) on the islands and their coordinates were measured with RTK surveying methods to increase the absolute accuracy of the project. We constructed 3D textured model using photogrammetry tool, which generates 3D spatial information from digital images. From the polygonal model, we could get DSM with contour lines. Thematic maps such as hill shade relief map, aspect map and slope map were also processed. Those maps make us understand topography condition of the project area better. The purpose of this project is monitoring topography change of these small islands. Elevation difference map between DSMs of each year is constructed. There are two regions showing big negative difference value. By comparing constructed textured models and captured digital images around these regions, it is checked that a region have experienced real topography change. It is due to huge rock fall near the center of the east island. The size of fallen rock can be measured on the digital model exactly, which is about 13m*6m*2m (height*width*thickness). We believe that drone aerial photogrammetry can be an efficient topography changes detection method for a complicated terrain area.

Evaluation of NOAA's High Resolution Rapid Refresh (HRRR), 12 km North America Model (NAM12) and 4km North America Model (NAM 4) hub-height wind speed forecasts

NASA Astrophysics Data System (ADS)

Pendergrass, W.; Vogel, C. A.

2013-12-01

As an outcome of discussions between Duke Energy Generation and NOAA/ARL following the 2009 AMS Summer Community Meeting, in Norman Oklahoma, ARL and Duke Energy Generation (Duke) signed a Cooperative Research and Development Agreement (CRADA) which allows NOAA to conduct atmospheric boundary layer (ABL) research using Duke renewable energy sites as research testbeds. One aspect of this research has been the evaluation of forecast hub-height winds from three NOAA atmospheric models. Forecasts of 10m (surface) and 80m (hub-height) wind speeds from (1) NOAA/GSD's High Resolution Rapid Refresh (HRRR) model, (2) NOAA/NCEP's 12 km North America Model (NAM12) and (3) NOAA/NCEP's 4k high resolution North America Model (NAM4) were evaluated against 18 months of surface-layer wind observations collected at the joint NOAA/Duke Energy research station located at Duke Energy's West Texas Ocotillo wind farm over the period April 2011 through October 2012. HRRR, NAM12 and NAM4 10m wind speed forecasts were compared with 10m level wind speed observations measured on the NOAA/ATDD flux-tower. Hub-height (80m) HRRR , NAM12 and NAM4 forecast wind speeds were evaluated against the 80m operational PMM27-28 meteorological tower supporting the Ocotillo wind farm. For each HRRR update, eight forecast hours (hour 01, 02, 03, 05, 07, 10, 12, 15) plus the initialization hour (hour 00), evaluated. For the NAM12 and NAM4 models forecast hours 00-24 from the 06z initialization were evaluated. Performance measures or skill score based on absolute error 50% cumulative probability were calculated for each forecast hour. HRRR forecast hour 01 provided the best skill score with an absolute wind speed error within 0.8 m/s of observed 10m wind speed and 1.25 m/s for hub-height wind speed at the designated 50% cumulative probability. For both NAM4 and NAM12 models, skill scores were diurnal with comparable best scores observed during the day of 0.7 m/s of observed 10m wind speed and 1.1 m/s for hub-height wind speed at the designated 50% cumulative probability level.

An unbiased approach to identify genes involved in development in a turtle with temperature-dependent sex determination.

PubMed

Chojnowski, Jena L; Braun, Edward L

2012-07-15

Many reptiles exhibit temperature-dependent sex determination (TSD). The initial cue in TSD is incubation temperature, unlike genotypic sex determination (GSD) where it is determined by the presence of specific alleles (or genetic loci). We used patterns of gene expression to identify candidates for genes with a role in TSD and other developmental processes without making a priori assumptions about the identity of these genes (ortholog-based approach). We identified genes with sexually dimorphic mRNA accumulation during the temperature sensitive period of development in the Red-eared slider turtle (Trachemys scripta), a turtle with TSD. Genes with differential mRNA accumulation in response to estrogen (estradiol-17β; E(2)) exposure and developmental stages were also identified. Sequencing 767 clones from three suppression-subtractive hybridization libraries yielded a total of 581 unique sequences. Screening a macroarray with a subset of those sequences revealed a total of 26 genes that exhibited differential mRNA accumulation: 16 female biased and 10 male biased. Additional analyses revealed that C16ORF62 (an unknown gene) and MALAT1 (a long noncoding RNA) exhibited increased mRNA accumulation at the male producing temperature relative to the female producing temperature during embryonic sexual development. Finally, we identified four genes (C16ORF62, CCT3, MMP2, and NFIB) that exhibited a stage effect and five genes (C16ORF62, CCT3, MMP2, NFIB and NOTCH2) showed a response to E(2) exposure. Here we report a survey of genes identified using patterns of mRNA accumulation during embryonic development in a turtle with TSD. Many previous studies have focused on examining the turtle orthologs of genes involved in mammalian development. Although valuable, the limitations of this approach are exemplified by our identification of two genes (MALAT1 and C16ORF62) that are sexually dimorphic during embryonic development. MALAT1 is a noncoding RNA that has not been implicated in sexual differentiation in other vertebrates and C16ORF62 has an unknown function. Our results revealed genes that are candidates for having roles in turtle embryonic development, including TSD, and highlight the need to expand our search parameters beyond protein-coding genes.

WE-AB-BRB-01: Development of a Probe-Format Graphite Calorimeter for Practical Clinical Dosimetry: Numerical Design Optimization, Prototyping, and Experimental Proof-Of-Concept

DOE Office of Scientific and Technical Information (OSTI.GOV)

Renaud, J; Seuntjens, J; Sarfehnia, A

2015-06-15

Purpose: In this work, the feasibility of performing absolute dose to water measurements using a constant temperature graphite probe calorimeter (GPC) in a clinical environment is established. Methods: A numerical design optimization study was conducted by simulating the heat transfer in the GPC resulting from irradiation using a finite element method software package. The choice of device shape, dimensions, and materials was made to minimize the heat loss in the sensitive volume of the GPC. The resulting design, which incorporates a novel aerogel-based thermal insulator, and 15 temperature sensitive resistors capable of both Joule heating and measuring temperature, was constructedmore » in house. A software based process controller was developed to stabilize the temperatures of the GPC’s constituent graphite components to within a few 10’s of µK. This control system enables the GPC to operate in either the quasi-adiabatic or isothermal mode, two well-known, and independent calorimetry techniques. Absorbed dose to water measurements were made using these two methods under standard conditions in a 6 MV 1000 MU/min photon beam and subsequently compared against TG-51 derived values. Results: Compared to an expected dose to water of 76.9 cGy/100 MU, the average GPC-measured doses were 76.5 ± 0.5 and 76.9 ± 0.5 cGy/100 MU for the adiabatic and isothermal modes, respectively. The Monte Carlo calculated graphite to water dose conversion was 1.013, and the adiabatic heat loss correction was 1.003. With an overall uncertainty of about 1%, the most significant contributions were the specific heat capacity (type B, 0.8%) and the repeatability (type A, 0.6%). Conclusion: While the quasi-adiabatic mode of operation had been validated in previous work, this is the first time that the GPC has been successfully used isothermally. This proof-of-concept will serve as the basis for further study into the GPC’s application to small fields and MRI-linac dosimetry. This work has been supported in part by the CREATE Medical Physics Research Training Network of the Natural Sciences and Engineering Research Council (NSERC) grant 432290, NSERC grants RGPIN 298191 & 435608-13, Canadian Institutes of Health Research doctoral scholarship GSD-121793. This work has also been supported by Sun Nuclear Corporation.« less

Multi-response calibration of a conceptual hydrological model in the semiarid catchment of Wadi al Arab, Jordan

NASA Astrophysics Data System (ADS)

Rödiger, T.; Geyer, S.; Mallast, U.; Merz, R.; Krause, P.; Fischer, C.; Siebert, C.

2014-02-01

A key factor for sustainable management of groundwater systems is the accurate estimation of groundwater recharge. Hydrological models are common tools for such estimations and widely used. As such models need to be calibrated against measured values, the absence of adequate data can be problematic. We present a nested multi-response calibration approach for a semi-distributed hydrological model in the semi-arid catchment of Wadi al Arab in Jordan, with sparsely available runoff data. The basic idea of the calibration approach is to use diverse observations in a nested strategy, in which sub-parts of the model are calibrated to various observation data types in a consecutive manner. First, the available different data sources have to be screened for information content of processes, e.g. if data sources contain information on mean values, spatial or temporal variability etc. for the entire catchment or only sub-catchments. In a second step, the information content has to be mapped to relevant model components, which represent these processes. Then the data source is used to calibrate the respective subset of model parameters, while the remaining model parameters remain unchanged. This mapping is repeated for other available data sources. In that study the gauged spring discharge (GSD) method, flash flood observations and data from the chloride mass balance (CMB) are used to derive plausible parameter ranges for the conceptual hydrological model J2000g. The water table fluctuation (WTF) method is used to validate the model. Results from modelling using a priori parameter values from literature as a benchmark are compared. The estimated recharge rates of the calibrated model deviate less than ±10% from the estimates derived from WTF method. Larger differences are visible in the years with high uncertainties in rainfall input data. The performance of the calibrated model during validation produces better results than applying the model with only a priori parameter values. The model with a priori parameter values from literature tends to overestimate recharge rates with up to 30%, particular in the wet winter of 1991/1992. An overestimation of groundwater recharge and hence available water resources clearly endangers reliable water resource managing in water scarce region. The proposed nested multi-response approach may help to better predict water resources despite data scarcity.

Subordinate-level object classification reexamined.

PubMed

Biederman, I; Subramaniam, S; Bar, M; Kalocsai, P; Fiser, J

1999-01-01

The classification of a table as round rather than square, a car as a Mazda rather than a Ford, a drill bit as 3/8-inch rather than 1/4-inch, and a face as Tom have all been regarded as a single process termed "subordinate classification." Despite the common label, the considerable heterogeneity of the perceptual processing required to achieve such classifications requires, minimally, a more detailed taxonomy. Perceptual information relevant to subordinate-level shape classifications can be presumed to vary on continua of (a) the type of distinctive information that is present, nonaccidental or metric, (b) the size of the relevant contours or surfaces, and (c) the similarity of the to-be-discriminated features, such as whether a straight contour has to be distinguished from a contour of low curvature versus high curvature. We consider three, relatively pure cases. Case 1 subordinates may be distinguished by a representation, a geon structural description (GSD), specifying a nonaccidental characterization of an object's large parts and the relations among these parts, such as a round table versus a square table. Case 2 subordinates are also distinguished by GSDs, except that the distinctive GSDs are present at a small scale in a complex object so the location and mapping of the GSDs are contingent on an initial basic-level classification, such as when we use a logo to distinguish various makes of cars. Expertise for Cases 1 and 2 can be easily achieved through specification, often verbal, of the GSDs. Case 3 subordinates, which have furnished much of the grist for theorizing with "view-based" template models, require fine metric discriminations. Cases 1 and 2 account for the overwhelming majority of shape-based basic- and subordinate-level object classifications that people can and do make in their everyday lives. These classifications are typically made quickly, accurately, and with only modest costs of viewpoint changes. Whereas the activation of an array of multiscale, multiorientation filters, presumed to be at the initial stage of all shape processing, may suffice for determining the similarity of the representations mediating recognition among Case 3 subordinate stimuli (and faces), Cases 1 and 2 require that the output of these filters be mapped to classifiers that make explicit the nonaccidental properties, parts, and relations specified by the GSDs.

Incident-response monitoring technologies for aircraft cabin air quality

NASA Astrophysics Data System (ADS)

Magoha, Paul W.

Poor air quality in commercial aircraft cabins can be caused by volatile organophosphorus (OP) compounds emitted from the jet engine bleed air system during smoke/fume incidents. Tri-cresyl phosphate (TCP), a common anti-wear additive in turbine engine oils, is an important component in today's global aircraft operations. However, exposure to TCP increases risks of certain adverse health effects. This research analyzed used aircraft cabin air filters for jet engine oil contaminants and designed a jet engine bleed air simulator (BAS) to replicate smoke/fume incidents caused by pyrolysis of jet engine oil. Field emission scanning electron microscopy (FESEM) with X-ray energy dispersive spectroscopy (EDS) and neutron activation analysis (NAA) were used for elemental analysis of filters, and gas chromatography interfaced with mass spectrometry (GC/MS) was used to analyze used filters to determine TCP isomers. The filter analysis study involved 110 used and 90 incident filters. Clean air filter samples exposed to different bleed air conditions simulating cabin air contamination incidents were also analyzed by FESEM/EDS, NAA, and GC/MS. Experiments were conducted on a BAS at various bleed air conditions typical of an operating jet engine so that the effects of temperature and pressure variations on jet engine oil aerosol formation could be determined. The GC/MS analysis of both used and incident filters characterized tri- m-cresyl phosphate (TmCP) and tri-p-cresyl phosphate (TpCP) by a base peak of an m/z = 368, with corresponding retention times of 21.9 and 23.4 minutes. The hydrocarbons in jet oil were characterized in the filters by a base peak pattern of an m/z = 85, 113. Using retention times and hydrocarbon thermal conductivity peak (TCP) pattern obtained from jet engine oil standards, five out of 110 used filters tested had oil markers. Meanwhile 22 out of 77 incident filters tested positive for oil fingerprints. Probit analysis of jet engine oil aerosols obtained from BAS tests by optical particle counter (OPC) revealed lognormal distributions with the mean (range) of geometric mass mean diameter (GMMD) = 0.41 (0.39, 0.45) microm and geometric standard deviation (GSD), sigma g = 1.92 (1.87, 1.98). FESEM/EDS and NAA techniques found a wide range of elements on filters, and further investigations of used filters are recommended using these techniques. The protocols for air and filter sampling and GC/MS analysis used in this study will increase the options available for detecting jet engine oil on cabin air filters. Such criteria could support policy development for compliance with cabin air quality standards during incidents.

Trace elements in starter infant formula: dietary intake and safety assessment.

PubMed

Bargellini, Annalisa; Venturelli, Francesco; Casali, Elisabetta; Ferrari, Angela; Marchesi, Isabella; Borella, Paola

2018-01-01

The aim of this study was to investigate the concentrations of five essential (Fe, Mn, Zn, Cu and Se) and four non-essential/toxic elements (Cr, Cd, Ni and Pb) in 35 different starter infant formulas (0-6 months) sold in Italy. In addition, a safety assessment of these trace elements was carried out, by comparing the estimated daily intake (EDI) with the adequate intake (AI) and the provisional tolerable daily intake (PTDI), with a view to provide information on the metal distribution patterns and health risk to infants arising from the consumption of these products. The concentrations were determined by using inductively coupled plasma mass spectrometry after microwave digestion. The concentrations expressed in geometric mean ± geometric standard deviation of Fe (6.17 ± 1.61 mg/L), Zn (6.21 ± 1.31 mg/L), Cu (416.4 ± 1.21 μg/L), Mn (121.5 ± 1.85 μg/L) and Se (13.27 ± 1.67 μg/L) were within legal limits. In spite of this, the mean EDIs of Fe (4.81 mg/day) and Mn (94.75 μg/day) were many times higher than the recommended AI, especially for Mn. Chromium, Ni, Cd and Pb concentrations were not detectable in 11, 37, 57 and 66% of the samples, respectively. Considering the overall sample, the GM ± GSD of these elements were 4.80 ± 5.35 μg/L for Cr, 1.02 ± 11.65 μg/L for Ni, 0.21 ± 14.83 μg/L for Cd and 0.14 ± 17.13 μg/L for Pb. The mean EDIs were far below the respective PTDI. When the safety assessment was based on the 75° percentile level of each elements, all EDIs remained well below the PTDI, with the exception of Cd, whose EDI approached (74.7%), albeit remaining below the PTDI. In conclusion, our results and the increased awareness on the potential risks of excessive Mn and Fe for infants support that an urgent scientific-based definition of the appropriated levels of fortification in formulas is required. Moreover, regular monitoring of all the stages of production of infant formulas is essential in order to limit toxic metal contamination.

RPAS application for estimating road exposition to rockfall

NASA Astrophysics Data System (ADS)

Santangelo, Michele; Alvioli, Massimiliano; Baldo, Marco; Giordan, Daniele; Guzzetti, Fausto; Marchesini, Ivan; Reichenbach, Paola

2017-04-01

The use of Remotely Piloted Aircraft Systems (RPASs) for landslide analysis and characterization is often aimed at the acquisition of DSMs and orthpohotos. One of the most interesting utilizations of RPASs to landslide studies consists in the production of data for rockfall risk assessment. A typical approach to study rockfalls consists in the application of numerical or stochastic models for the definition of possible trajectories of rock blocks to accurate DTMs of the source and runout areas. In this work, the case study of the rockfall of Vinnanova di Accumoli (Marche Region, central Italy) is presented and discussed. In this area, the earthquakes of the seismic sequence started on 24 August 2016 that struck central Italy caused several rockfalls that, in some cases damaged roads, and represented a threat to the population. In particular, the provincial road SP18 near Villanova di Accumoli was closed due to a 1 m3 rock block that fell down from the slope and crossed the SP20, partially damaging it. During the emergency, it was decided to apply a numerical model to estimate the trajectories of the remaining instable rock masses and to define the possible places where to set up protection measures to safely re-open the road. Therefore, a survey with a multicopter was carried out to obtain (i) an accurate DSM of the source area and the slope (ii) the identification and characterization of other instable blocks possibly not visible in the field. The 6,500 m2 area was covered by a total 161 photograms by a 34 Mpixel camera, obtaining a 1.5 cm/pixel Ground Sampling Distance (GSD). The final orthophoto has a resolution of 2.5 cm, whereas the DSM has a resolution of 20 cm. The DSM was then filtered by a three-step procedure including manual removal of sparse vegetation cover. In area covered by dense vegetation (the lower part of the slope) the DSM could not be manually filtered, which hampered to run the numerical model. This problem was addressed by a GPS RTK survey of the most vegetated area. A total of 73 points with less than 1m error were acquired and integrated in the DTM. The resulting integrated DTM has a resolution of 25 cm. The numerical model STONE was then applied to the source areas mapped in the field and by photo-interpretation of the RPAS orthophoto to get a 1m raster showing the potential trajectories of the mapped instable rock masses. Results showed that only the part of the road hit by the rockfall was actually exposed to rockfall trajectories. Therefore only limited protection measures were suggested to reduce the exposition of the road.

Classification of non native tree species in Adda Park (Italy) through multispectral and multitemporal surveys from UAV

NASA Astrophysics Data System (ADS)

Pinto, Livio; Sona, Giovanna; Biffi, Andrea; Dosso, Paolo; Passoni, Daniele; Baracani, Matteo

2014-05-01

The project ITACA (Innovation, Technologies, Actions to Contrast Alloctonous species) rises from the need of protecting natural habitats in parks where native vegetation is threaten by the always increasing spread of alloctonous species. Starting from preliminary results obtained in previous experimental studies performed inside Adda Park (Lombardy Region, Northern Italy) the aim of the project is a further development and optimization of some tested techniques and procedures. In the frame of ITACA project, that involves Politecnico di Milano and some local enterprises, 11 separate areas of the Adda Park, globally covering 50 hectars, will be surveyed with UAV-borne multispectral sensors through different seasons (summer, autumn and spring). The summer and autumn flights have already been realized by the fixed wing UAV Sensefly SwingletCAM mounted with a Canon Ixus 220HS, producing real color images (RGB), and an identical camera, modified to produce false color images (NIR-RG). The 'multisensor-multitemporal' flights have been planned with high longitudinal and transversal overlaps, always in the range 60% to 80%, and a GSD of around 4 cm. Presignalized artificial points or natural elements have been surveyed on the ground by GPS RTK Trimble 5700, making use a Network GPS ervice (NRTK). For each survey two flights have been realized, one with the standard camera, and the second one with the NIR-modified one, with the double purpose of: - producing a multispectral orthomosaic, formed by the four bands NIR-R-G-B, coregistered. - increasing the coverage of the area, yielding in the block adjustment phase a more robust solution and a higher metric accuracy of digital products (digital orthomosaics). The first two flights have been scheduled taking into account information on the phenology of the species under observation (both native or invasive) given by expert botanists involved in the project. The first set of acquisition, originally planned for the first half of July, was realized over a longer period : from 09/07/2013 to 28/08/2013, due to weather condition and technical reasons. In any case the vegetation characteristics resulted to be unchanged. The second set of flights, in autumn, were done in a shorter period, during the days 16-17-18 October 2013, thus obtaining even better homogeneity of the vegetation conditions. Image and data processing are based on standard classification techniques, both pixel and object based, applied simultaneously on multispectral and multitemporal data, with the aim of producing a thematic map of the species of interest. The classification accuracies will be computed on the basis of ground truth comparison, to study possible misclassification among species.

Monitoring Seabirds and Marine Mammals by Georeferenced Aerial Photography

NASA Astrophysics Data System (ADS)

Kemper, G.; Weidauer, A.; Coppack, T.

2016-06-01

The assessment of anthropogenic impacts on the marine environment is challenged by the accessibility, accuracy and validity of biogeographical information. Offshore wind farm projects require large-scale ecological surveys before, during and after construction, in order to assess potential effects on the distribution and abundance of protected species. The robustness of site-specific population estimates depends largely on the extent and design of spatial coverage and the accuracy of the applied census technique. Standard environmental assessment studies in Germany have so far included aerial visual surveys to evaluate potential impacts of offshore wind farms on seabirds and marine mammals. However, low flight altitudes, necessary for the visual classification of species, disturb sensitive bird species and also hold significant safety risks for the observers. Thus, aerial surveys based on high-resolution digital imagery, which can be carried out at higher (safer) flight altitudes (beyond the rotor-swept zone of the wind turbines) have become a mandatory requirement, technically solving the problem of distant-related observation bias. A purpose-assembled imagery system including medium-format cameras in conjunction with a dedicated geo-positioning platform delivers series of orthogonal digital images that meet the current technical requirements of authorities for surveying marine wildlife at a comparatively low cost. At a flight altitude of 425 m, a focal length of 110 mm, implemented forward motion compensation (FMC) and exposure times ranging between 1/1600 and 1/1000 s, the twin-camera system generates high quality 16 bit RGB images with a ground sampling distance (GSD) of 2 cm and an image footprint of 155 x 410 m. The image files are readily transferrable to a GIS environment for further editing, taking overlapping image areas and areas affected by glare into account. The imagery can be routinely screened by the human eye guided by purpose-programmed software to distinguish biological from non-biological signals. Each detected seabird or marine mammal signal is identified to species level or assigned to a species group and automatically saved into a geo-database for subsequent quality assurance, geo-statistical analyses and data export to third-party users. The relative size of a detected object can be accurately measured which provides key information for species-identification. During the development and testing of this system until 2015, more than 40 surveys have produced around 500.000 digital aerial images, of which some were taken in specially protected areas (SPA) of the Baltic Sea and thus include a wide range of relevant species. Here, we present the technical principles of this comparatively new survey approach and discuss the key methodological challenges related to optimizing survey design and workflow in view of the pending regulatory requirements for effective environmental impact assessments.

Production in Gdlrc and Present Reflections

NASA Astrophysics Data System (ADS)

Kisa, A.; Çolak, S.; Bakici, S.; Özmüş, L.

2013-08-01

Recently, Turkey's National Geographic Information System (TNGIS) carried out a more comprehensive studies. General Directorate of Land Registry and Cadastre (GDLRC) within the scope of these studies has projects in many areas within the jurisdiction. GDLRC have started Land Registry and Cadastre Modernization Project (LRCMP) in 2008 and still continues this project. The current project is very successful in the renewal and transfer of digital media, after the completion of the country's digital cadastre. The scope of this project was prepared comprehensive study. The scope of this project was prepared comprehensive study. This studies; human resources development, new cadastre offices renovation and its services improvement, examining and reporting of the valuation of real estate, renovation and updating of the cadastre. All works continues at the same speed and determination. With these developments, GDLRC works with institutions, organizations and citizens. These developments cause a further increase interoperability and trust relationships. GDLRC, across the country, produces, stores, manages and preservesof property information. GDLRC the use and development of real estate is an important way to the work. In this context, one of the layers of spatial information systems, an essential requirement of the images which obtained by means of remote sensing satellite photos and/or consist of aerial photographs, is quite an important role. In research, in order to meet the common needs of different institutions and organizations, aerial photographs and orthophoto imagery are needed. Aerial photographs more up to date, precise, clear and reliable. GDLRC, signing of important projects, is working to implement the Orthophoto Information System (OIS) project. GDLRC equipped with a new photogrammetric systems in 2009. In this way, the technological advances in the industry leading on this issue very closely monitored and carried out the task successfully. 200 000 km2 area in the country, 1/5000 scaled digital color orthophoto images were producedbetween 2009-2012. Orthophoto images of other areas is carried out by General Command of Mapping (GCM). Orthophotos , base images with the current covering the whole country, cloudless, 30-45 cm in the sampling interval (Ground Sample Distance - GSD), produced will be realized by both institutions in 2014. Ongoing projects in the fields covered by the renewal is important. Orthophoto production stage, stereo, color, and near-infrared aerial photographs of the terrain elevation models are also available. Municipalities places by 1/1000 scale orthophoto images prepared in this process. The production of orthophotos, digital cadastral works of engineering projects and other institutions, decision-support processes, quality controls and integrity of the legal dimension of the feature can be used to create litter. GDLRC for these purposes by the OGC Web Services standards TNGIS prepared and successfully created the image layer. Two projects planned by GDLRC. In both of projects, historical aerial photographs, are retrieved from GDLRC and GCM archives, will scan, produce orthophoto and service from web.

Hydrological regime as key to the morpho-texture and activity of braided streams

NASA Astrophysics Data System (ADS)

Storz-Peretz, Y.; Laronne, J. B.

2012-04-01

Braided streams are a common fluvial pattern in different climates. However, studies of gravel braided streams have mainly been conducted in humid braided systems or in flume simulations thereof, leaving arid braided streams scarcely investigated. Dryland rivers have bare catchments, rapid flow recession and unarmoured channel beds which are responsible for very high bedload discharges, thereby increasing the likelihood for braiding. Our main objective is to characterize the morpho-texture of the main morphological elements - mid-channel bars, chutes and anabranches (braid-cells) in the dryland braided system and compare them to their humid counterparts. Selected areas of the dryland braided Wadis Ze'elim, Rahaf and Roded in the SE hyper-arid Israel were measured, as were La-Bleone river in the French pre-alps along with the Saisera and Cimoliana rivers in NE Italy representing humid braided systems. Terrestrial Laser Scanning (TLS) of morphological units produced point clouds from which high resolution accurate Digital Elevation Models (DEMs) were extracted. Active braid cells in humid environments were also surveyed by electronic theodolite. Roughness and upper tail Grain Size Distribution (GSD) quantiles were derived from the scanned point clouds or from Wolman sampling. Results indicate that dryland anabranches tend to be finer-grained and less armoured than the bars, contrary to the humid braided systems, where the main or larger anabranches are coarser-grained and more armoured than the bars. Chutes are commonly similar or coarser-grained than the bars they dissect, in accordance with their steeper gradients due to the considerable relief of the bar-anabranch. The morpho-texture displayed in the steep braided Saisera River, located in the Italian Dolomites having the highest annual precipitation, has similarity to that of the dryland braided channels. In drylands coarse gravel is deposited mainly as bars due to the high flux of bedload, whereas the rapid flow recession is responsible for deposition of finer sediment with minimal winnowing in the branch channels. Therefore, channels are finer-grained than the bars. This process is associated with the mid-channel deposition of central bars. However, the steeper chutes and coarser anabranches are associated with erosive braiding processes, such as chute cutoffs and multiple bar dissection, allowing winnowing to occur also during rapid recession. Hence coarser-grained anabranches in drylands are essentially chutes. Lengthy flow recession in humid braided channels allows winnowing of fines, thereby generating armored channels, the finer sedimentary particles often deposited downstream as unit bars. Therefore, channels are coarser-grained than the bars they surround. Even though the steep Saisera is in a humid region, its hydrological regime is ephemeral with rapid and short recessions, responsible for a morpho-texture similar to that of dryland braided streams. Hence, the hydrologic regimen is a key to understanding the morpho-textural character of braided channels and for the higher activity of the ephemeral unarmoured channels in sub-barful events compared to their humid counterparts.

Photogrammetric Processing Using ZY-3 Satellite Imagery

NASA Astrophysics Data System (ADS)

Kornus, W.; Magariños, A.; Pla, M.; Soler, E.; Perez, F.

2015-03-01

This paper evaluates the stereoscopic capacities of the Chinese sensor ZiYuan-3 (ZY-3) for the generation of photogrammetric products. The satellite was launched on January 9, 2012 and carries three high-resolution panchromatic cameras viewing in forward (22º), nadir (0º) and backward direction (-22º) and an infrared multi-spectral scanner (IRMSS), which is slightly looking forward (6º). The ground sampling distance (GSD) is 2.1m for the nadir image, 3.5m for the two oblique stereo images and 5.8m for the multispectral image. The evaluated ZY-3 imagery consists of a full set of threefold-stereo and a multi-spectral image covering an area of ca. 50km x 50km north-west of Barcelona, Spain. The complete photogrammetric processing chain was executed including image orientation, the generation of a digital surface model (DSM), radiometric image correction, pansharpening, orthoimage generation and digital stereo plotting. All 4 images are oriented by estimating affine transformation parameters between observed and nominal RPC (rational polynomial coefficients) image positions of 17 ground control points (GCP) and a subsequent calculation of refined RPC. From 10 independent check points RMS errors of 2.2m, 2.0m and 2.7m in X, Y and H are obtained. Subsequently, a DSM of 5m grid spacing is generated fully automatically. A comparison with the Lidar data results in an overall DSM accuracy of approximately 3m. In moderate and flat terrain higher accuracies in the order of 2.5m and better are achieved. In a next step orthoimages from the high resolution nadir image and the multispectral image are generated using the refined RPC geometry and the DSM. After radiometric corrections a fused high resolution colour orthoimage with 2.1m pixel size is created using an adaptive HSL method. The pansharpen process is performed after the individual geocorrection due to the different viewing angles between the two images. In a detailed analysis of the colour orthoimage artifacts are detected covering an area of 4691ha, corresponding to less than 2% of the imaged area. Most of the artifacts are caused by clouds (4614ha). A minor part (77ha) is affected by colour patch, stripping or blooming effects. For the final qualitative analysis on the usability of the ZY-3 imagery for stereo plotting purposes stereo combinations of the nadir and an oblique image are discarded, mainly due to the different pixel size, which produces difficulties in the stereoscopic vision and poor accuracy in positioning and measuring. With the two oblique images a level of detail equivalent to 1:25.000 scale is achieved for transport network, hydrography, vegetation and elements to model the terrain as break lines. For settlement, including buildings and other constructions a lower level of detail is achieved equivalent to 1:50.000 scale.

Mapping the Wetland Vegetation Communities of the Australian Great Artesian Basin Springs Using SAM, Mtmf and Spectrally Segmented PCA Hyperspectral Analyses

NASA Astrophysics Data System (ADS)

White, D. C.; Lewis, M. M.

2012-07-01

The Australian Great Artesian Basin (GAB) supports a unique and diverse range of groundwater dependent wetland ecosystems termed GAB springs. In recent decades the ecological sustainability of the springs has become uncertain as demands on this iconic groundwater resource increase. The impacts of existing water extractions for mining and pastoral activities are unknown. This situation is compounded by the likelihood of future increasing demand for extractions. Hyperspectral remote sensing provides the necessary spectral and spatial detail to discriminate wetland vegetation communities. Therefore the objectives of this paper are to discriminate the spatial extent and distribution of key spring wetland vegetation communities associated with the GAB springs evaluating three hyperspectral techniques: Spectral Angle Mapper (SAM), Mixture Tuned Matched Filtering (MTMF) and Spectrally Segmented PCA. In addition, to determine if the hyperspectral techniques developed can be applied at a number of sites representative of the range of spring formations and geomorphic settings and at two temporal intervals. Two epochs of HyMap airborne hyperspectral imagery were captured for this research in March 2009 and April 2011 at a number of sites representative of the floristic and geomorphic diversity of GAB spring groups/complexes within South Australia. Colour digital aerial photography at 30 cm GSD was acquired concurrently with the HyMap imagery. The image acquisition coincided with a field campaign of spectroradiometry measurements and a botanical survey. To identify key wavebands which have the greatest capability to discriminate vegetation communities of the GAB springs and surrounding area three hyperspectral data reduction techniques were employed: (i) Spectrally Segmented PCA (SSPCA); (ii) the Minimum Noise Transform (MNF); and (iii) the Pixel Purity Index (PPI). SSPCA was applied to NDVI-masked vegetation portions of the HyMap imagery with wavelength regions spectrally segmented for the VIS-NIR (450-1,350 nm), SWIR 1 (1,400-1,800 nm) and SWIR 2 (1,950-2,480 nm). The resulting pure endmember image pixels of the vegetation communities identified were used as target spectra for input into the SAM and MTMF algorithms. Spring wetland vegetation communities successfully discriminated include low lying reeds and sedges along spring tails (Baumea spp. and Cyperus spp.), dense homogenous stands of Phragmites australis reeds, and sporadic patches of salt couch grass (Sparabolus spp.). Our results indicate that a combination of hyperspectral remote sensing techniques which reduce superfluous wavebands providing a targeted spectral matching approach are capable of discriminating and mapping key vegetation communities of the GAB springs. This approach provides reliable baseline mapping of the GAB spring wetland vegetation communities, with repeatability over space and time. In addition it has the capability to determine the sensitivity of spring wetland vegetation extent, distribution and diversity, to associated changes in spring flow rates due to water extractions. This approach will ultimately inform water allocation plan management policies.

Forest STEM Volume Calculation Using Airborne LIDAR Data

NASA Astrophysics Data System (ADS)

Büyüksalih, I.; Bayburt, S.; Schardt, M.; Büyüksalih, G.

2017-05-01

Airborne LiDAR data have been collected for the city of Istanbul using Riegl laser scanner Q680i with 400 kHz and an average flight height of 600 m. The flight campaign was performed by a helicopter and covers an area of 5400 km2. According to a flight speed of 80 knot a point density of more than 16 points/m2 and a laser footprint size of 30 cm could be achieved. As a result of bundle adjustment, in total, approximately 17,000 LAS files with the file size of 500 m by 700 m have been generated for the whole city. The main object classes Ground, Building, Vegetation (medium, high) were derived from these LAS files using the macros in Terrasolid software. The forest area under investigation is located northwest of the city of Istanbul, main tree species occurring in the test site are pine (pinus pinaster), oak (quercus) and beech (fagus). In total, 120 LAS tiles covering the investigation area have been analysed using the software IMPACT of Joanneum Research Forschungsgesellschaft, Graz, Austria. First of all, the digital terrain model (DTM) and the digital surface models (DSM) were imported and converted into a raster file from the original laser point clouds with a spatial resolution of 50 cm. Then, a normalized digital surface model (nDSM) was derived as the difference between DSM and the DTM. Tree top detection was performed by multi - resolution filter operations and tree crowns were segmented by a region growing algorithms develop specifically for this purpose. Breast Height Diameter (BHD) was calculated on the base of tree height and crown areas derived from image segmentation applying allometric functions found in literature. The assessment of stem volume was then calculated as a function of tree height and BHD. A comparison of timber volume estimated from the LiDAR data and field plots measured by the Forest Department of Istanbul showed R2 of 0.46. The low correlation might arise either from the low quality of the field plots or from the inadequacy of the allometric functions used for BHD and stem volume modelling. Further investigations therefore will concentrate both on improving the quality of field measurements and the adoption of the allometric functions to the specific site condition of the forests under investigation. Finally stand boundaries of the forest area made available by the forest department of Istanbul were superimposed to the LiDAR data and the single tree measurements aggregated to the stand level. Aside from the LiDAR data, a Pleiades multispectral image characterized by four spectral bands (blue, green, red and near infrared) and a GSD of 2.8 m has been used for the classification of different tree species. For this purpose the near infrared band covering the spectral range of 0.75 μm to 0.90 μm has been utilized and the IMPACT software used.

Coupling effect analysis between landslides, river channel changes and sediment budgets - extreme climate events in Laishe River, southern Taiwan

NASA Astrophysics Data System (ADS)

Chang, Kuo-Jen; Huang, Mei-Jen; Tseng, Chih-Ming

2016-04-01

Taiwan, due to the high seismicity and high annual rainfall, numerous landslides triggered every year and severe impacts affect the island. Concerning to the catastrophic landslides, the key information of landslide, including range of landslide, volume estimation and the subsequent evolution are important when analyzing the triggering mechanism, hazard assessment and mitigation. Thus, the morphological analysis gives a general overview for the landslides and been considered as one of the most fundamental information. Typhoon Morakot brought extreme and long-time rainfall for Taiwan in August 2009, and caused severe disasters. In this study we integrate several technologies, especially by Unmanned Aerial Vehicle (UAV) and multi-spectral camera, to decipher the consequence and the potential hazard, and the social impact. In recent years, the remote sensing technology improves rapidly, providing a wide range of image, essential and precise information. This study integrates several methods, including, 1) Remote-sensing images gathered by Unmanned Aerial Vehicle (UAV) and by aerial photos taken in different periods; 2) field in-situ geologic investigation; 3) Differential GPS, RTK GPS geomatic measurements. The methods allow to constructing the DTMs before and after landslide, as well as the subsequent periods by using aerial photos and UAV derived images. The data sets permits to analysis the morphological changes. In the past, the study of sediment budgets usually relies on field investigation, but due to inconvenient transportation, topographical barriers, or located in remote areas, etc. the survey is hardly to be completed sometimes. In recent years, the rapid development of remote sensing technology improves image resolution and quality significantly. Remote sensing technology can provide a wide range of image data, and provide essential and precious information. The purpose of this study is to investigate the phenomenon of river migration and to evaluate the amount of migration along Laishe River by analyzing the 3D DEM before and after the typhoon Morakot. The DEMs are built by using the aerial images taken by digital mapping camera (DMC) and by airborne digital scanner 40 (ADS40) before and after typhoon event. Recently, this research integrates Unmanned Aerial Vehicle (UAV) and oblique photogrammetric technologies for image acquisition by 5-10cm GSD photos. This approach permits to construct true 3D model so as to decipher ground information more realistically. 10-20cm DSM and DEM, and field GPS, were compiled together to decipher the morphologic changes. All the information, especially by means of true 3D model, the datasets provides detail ground information that may use to evaluate the landslide triggering mechanism and river channel evolution. The goals of this study is to integrates the UAS system and to decipher the sliding process and morphologic changes of large landslide areas, sediment transport and budgets, and to investigate the phenomenon of river migration. The results of this study provides not only geomatics and GIS dataset of the hazards, but also for essential geomorphologic information for other study, and for hazard mitigation and planning, as well.

RPAS Monitoring of the Morphological Evolution of Coastal Foredunes

NASA Astrophysics Data System (ADS)

Taddia, Yuri; Corbau, Corinne; Elena, Zambello; Russo, Valentina; Pellegrinelli, Alberto; Simeoni, Umberto

2016-04-01

The coastal environment is in rapid and continuous evolution and it is easily affected by many natural and antropic factors. Beaches are often backed by vegetated dunes and fulfill many different valuable ecosystem functions. They act as protective buffers against storm surge, wave attack and erosion, providing a unique habitat for flora and fauna. Coastal embryo dunes, found above mean high water, are dynamic landform being able to supply sand to the beach when needed. They may form rapidly and may be rapidly destructed due to high tides and storm waves or human interferences. The southern part (3 km long) of Rosolina (Adriatic Sea, Italy) is characterized by a wide beach bordered by a complex dune system. The geomorphological characteristics of embryo dunes have been identified by using an RPAS in order to develop a fast and low-cost surveying technique. The aircraft has flown at a 50 meters altitude, taking photos with a 12Mpix RGB camera and a GSD of about 1 cm. The images overlap of 80% in the flight direction and 60% laterally. Fourteen targets have been collocated in the area as ground control points and were surveyed using Network Real Time Kinematic (NRTK) GNSS. Images and GCPs were elaborated in Agisoft PhotoScan to generate the model. A similar NRTK survey has been performed to integrate the wrong data (due to vegetation) for the creation of a digital elevation model (DEM) in a first step and finally to validate the model obtained through UAV photogrammetry through a comparison with specially surveyed points. The creation of a DEM from photos is one of main tasks and its accuracy is critical. A challenge in this work was to recognize the vegetation in the sand dunes area to exclude all the points not belonging to the ground. This was possible through a classification process based on slope detection. Finally, the suitable elevation accuracy has been reached and the survey has revealed a complex dune system characterized by: • on the upper part of the beach (about 1.50 meter amsl), isolated embryo dunes (incipient foredunes) and foredunes develop along a cross-shore section of about 30 meters; • a "depresssive" interdune of about 10 meters; • a second field of successive well-developed dunes. The first foredunes are of small dimensions (about 0.50 m height), and are aligned to the prevailing wind directions. The second field of dunes are higher (about 1 meter height) and form a "continuous" dune ridge parallel to the shoreline. Another significant advantage of this method is the possibility to obtain information on the vegetation, which is an important factor driving the evolution of coastal dunes. Different blowouts and pathways have been also mapped. The use of the orthophoto will therefore allow to collect simultaneously physical and biological data and consequently to analyze their interaction in order to improve the management of the coastal dune systems. This study exhibits how RPAS can be successfully used in representing the spatial distribution of embryo coastal dunes and in monitoring their development.

Carcinogenesis From Inhaled (PuO2)-Pu-239 in Beagles: Evidence for Radiation Homeostasis at Low Doses?

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fisher, Darrell R.; Weller, Richard E.

From the early 1970s to the late 1980s, Pacific Northwest National Laboratory conducted life-span studies in beagle dogs on the biological effects of inhaled plutonium (239PuO2, 238PuO2, and 239Pu[NO3]4) to help predict risks associated with accidental intakes in workers. Years later, the purpose of the present follow-up study is to reassess the dose-response relationship for lung cancer induction in the 239PuO2 dogs compared to controls, with particular focus on the dose-response at low lung doses. A 239PuO2 aerosol (2.3 μm AMAD, 1.9 μm GSD) was administered to six groups of 20 young (18-month old) beagle dogs (10 males and 10more » females) by inhalation at six different activity levels, as previously described in Laboratory reports. Control dogs were sham-exposed. In dose level 1, initial pulmonary lung depositions were 130 ± 48 Bq (3.5 ± 1.3 nCi), corresponding to 1 Bq g-1 lung tissue (0.029 ± 0.001 nCi g-1. Groups 2 through 6 received initial lung depositions (mean values) of 760, 2724, 10345, 37900, and 200000 Bq (22, 79, 300, 1100, and 5800 nCi) 239PuO2, respectively. For each dog, the absorbed dose to lungs was calculated from the initial lung burden and the final lung burden at time of death and lung mass, assuming a single, long-term retention function. Insoluble plutonium oxide exhibited long retention times in the lungs. Increased dose-dependent mortality due to lung cancer (bronchiolar-alveolar carcinoma, adenocarcinoma, epidermoid carcinoma) and radiation pneumonitis (highest exposures group) was observed in dogs exposed to 239PuO2. Calculated lung doses ranged from a few cGy in early-sacrificed dogs to 7764 cGy in dogs that experienced early deaths from radiation pneumonitis. Data were regrouped by lifetime lung dose and plotted as a function of lung tumor incidence. Lung tumor incidence in controls and zero-dose exposed dogs was 18% (5/28). However, no lung tumors were observed in 16 dogs with the lowest lung doses (8 to 22 cGy, mean 14.4 ± 7.6 cGy), and only one lung tumor was observed in 10 dogs with lung doses ranging from 27 to 48 cGy (mean 37.5 ± 10.9 cGy). By least-squares analysis, a quadratic function represented the overall dose-response (n = 137, r = 0.96) with no dose threshold. Reducing this function to three linear dose-response components, risk coefficients were calculated for each. The incidence of lung tumors at zero dose was significantly greater than the incidence at low dose (at the p ≤ 0.053 confidence level), suggesting a protective effect (radiation homeostasis) of alpha-particle radiation from 239PuO2. If a threshold for lung cancer incidence exists, it will be observed in the range 15 to 40 cGy.« less

Operational SAR Data Processing in GIS Environments for Rapid Disaster Mapping

NASA Astrophysics Data System (ADS)

Bahr, Thomas

2014-05-01

The use of SAR data has become increasingly popular in recent years and in a wide array of industries. Having access to SAR can be highly important and critical especially for public safety. Updating a GIS with contemporary information from SAR data allows to deliver a reliable set of geospatial information to advance civilian operations, e.g. search and rescue missions. SAR imaging offers the great advantage, over its optical counterparts, of not being affected by darkness, meteorological conditions such as clouds, fog, etc., or smoke and dust, frequently associated with disaster zones. In this paper we present the operational processing of SAR data within a GIS environment for rapid disaster mapping. For this technique we integrated the SARscape modules for ENVI with ArcGIS®, eliminating the need to switch between software packages. Thereby the premier algorithms for SAR image analysis can be directly accessed from ArcGIS desktop and server environments. They allow processing and analyzing SAR data in almost real time and with minimum user interaction. This is exemplified by the November 2010 flash flood in the Veneto region, Italy. The Bacchiglione River burst its banks on Nov. 2nd after two days of heavy rainfall throughout the northern Italian region. The community of Bovolenta, 22 km SSE of Padova, was covered by several meters of water. People were requested to stay in their homes; several roads, highways sections and railroads had to be closed. The extent of this flooding is documented by a series of Cosmo-SkyMed acquisitions with a GSD of 2.5 m (StripMap mode). Cosmo-SkyMed is a constellation of four Earth observation satellites, allowing a very frequent coverage, which enables monitoring using a very high temporal resolution. This data is processed in ArcGIS using a single-sensor, multi-mode, multi-temporal approach consisting of 3 steps: (1) The single images are filtered with a Gamma DE-MAP filter. (2) The filtered images are geocoded using a reference DEM without the need of ground control points. This step includes radiometric calibration. (3) A subsequent change detection analysis generates the final map showing the extent of the flash flood on Nov. 5th 2010. The underlying algorithms are provided by three different sources: Geocoding & radiometric calibration (2) is a standard functionality from the commercial SARscape Toolbox for ArcGIS. This toolbox is extended by the filter tool (1), which is called from the SARscape modules in ENVI. The change detection analysis (3) is based on ENVI processing routines and scripted with IDL. (2) and (3) are integrated with ArcGIS using a predefined Python interface. These 3 processing steps are combined using the ArcGIS ModelBuilder to create a new model for rapid disaster mapping in ArcGIS, based on SAR data. Moreover, this model can be dissolved from its desktop environment and published to users across the ArcGIS Server enterprise. Thus disaster zones, e.g. after severe flooding, can be automatically identified and mapped to support local task forces - using an operational workflow for SAR image analysis, which can be executed by the responsible operators without SAR expert knowledge.

Determination of Fluorine in Fourteen Microanalytical Geologic Reference Materials using SIMS, EPMA, and Proton Induced Gamma Ray Emission (PIGE) Analysis

NASA Astrophysics Data System (ADS)

Guggino, S. N.; Hervig, R. L.

2010-12-01

Fluorine (F) is a volatile constituent of magmas and hydrous minerals, and trace amounts of F are incorporated into nominally anhydrous minerals such as olivine and clinopyroxene. Microanalytical techniques are routinely used to measure trace amounts of F at both high sensitivity and high spatial resolution in glasses and crystals. However, there are few well-established F concentrations for the glass standards routinely used in microanalytical laboratories, particularly standards of low silica, basaltic composition. In this study, we determined the F content of fourteen commonly used microanalytical glass standards of basaltic, intermediate, and rhyolitic composition. To serve as calibration standards, five basaltic glasses with ~0.2 to 2.5 wt% F were synthesized and characterized. A natural tholeiite from the East Pacific Rise was mixed with variable amounts of CaF2. The mixture was heated in a 1 atmosphere furnace to 1440 °C at fO2 = NNO for 30 minutes and quenched in water. Portions of the run products were studied by electron probe microanalysis (EPMA) and secondary ion mass spectrometry (SIMS). The EPMA used a 15 µm diameter defocused electron beam with a 15 kV accelerating voltage and a 25 nA primary current, a TAP crystal for detecting FKα X-rays, and Biotite 3 as the F standard. The F contents by EPMA agreed with the F added to the basalts after correction for mass loss during melting. The SIMS analyses used a primary beam of 16O- and detection of low-energy negative ions (-5 kV) at a mass resolution that resolved 18OH. Both microanalytical techniques confirmed homogeneity, and the SIMS calibration defined by EPMA shows an excellent linear trend with backgrounds of 2 ppm or less. Analyses of basaltic glass standards based on our synthesized calibration standards gave the following F contents and 2σ errors (ppm): ALV-519 = 83 ± 3; BCR-2G = 359 ± 6; BHVO-2G = 322 ± 15; GSA-1G = 10 ± 1; GSC-1G = 11 ± 1; GSD-1G = 19 ± 2; GSE-1G = 173 ± 1; KL2G (MPI-DING) = 101 ± 1; ML3B-G (MPI-DING) = 49 ± 17. These values are lower than published values for BCR-2 and BHVO-2 (unmelted powders) and the “information values” for the MPI-DING glass standards. Proton Induced Gamma ray Emission (PIGE) was tested for the high silica samples. PIGE analyses (1.7 MeV Tandem Accelerator; reaction type: 19F(p, αγ)16O; primary current = 20-30 nA; incident beam voltage = 1.5 MeV) were calibrated with a crystal of fluor-topaz (F = 20.3 wt%) and gave F values of: NIST 610 = 266 ± 14 ppm; NIST 620 = 54 ± 5 ppm; and UTR-2 = 1432 ± 32 ppm. SIMS calibration defined by the PIGE analyses shows an excellent linear trend with low background similar to the basaltic calibration. The F concentrations of intermediate MPI-DING glasses were determined based on SIMS calibration generated from the PIGE analysis above. The F concentrations and 2σ errors (ppm) are: T1G = 219.9 ± 6.8; StHs/680-G = 278.0 ± 2.0 ppm. This study revealed a large matrix effect between the high-silica and basaltic glasses, thus requiring the use of appropriate standards and separate SIMS calibrations when analyzing samples of different compositions.

Proceedings of the 2004 High Spatial Resolution Commercial Imagery Workshop

NASA Technical Reports Server (NTRS)

2006-01-01

Topics covered include: NASA Applied Sciences Program; USGS Land Remote Sensing: Overview; QuickBird System Status and Product Overview; ORBIMAGE Overview; IKONOS 2004 Calibration and Validation Status; OrbView-3 Spatial Characterization; On-Orbit Modulation Transfer Function (MTF) Measurement of QuickBird; Spatial Resolution Characterization for QuickBird Image Products 2003-2004 Season; Image Quality Evaluation of QuickBird Super Resolution and Revisit of IKONOS: Civil and Commercial Application Project (CCAP); On-Orbit System MTF Measurement; QuickBird Post Launch Geopositional Characterization Update; OrbView-3 Geometric Calibration and Geopositional Accuracy; Geopositional Statistical Methods; QuickBird and OrbView-3 Geopositional Accuracy Assessment; Initial On-Orbit Spatial Resolution Characterization of OrbView-3 Panchromatic Images; Laboratory Measurement of Bidirectional Reflectance of Radiometric Tarps; Stennis Space Center Verification and Validation Capabilities; Joint Agency Commercial Imagery Evaluation (JACIE) Team; Adjacency Effects in High Resolution Imagery; Effect of Pulse Width vs. GSD on MTF Estimation; Camera and Sensor Calibration at the USGS; QuickBird Geometric Verification; Comparison of MODTRAN to Heritage-based Results in Vicarious Calibration at University of Arizona; Using Remotely Sensed Imagery to Determine Impervious Surface in Sioux Falls, South Dakota; Estimating Sub-Pixel Proportions of Sagebrush with a Regression Tree; How Do YOU Use the National Land Cover Dataset?; The National Map Hazards Data Distribution System; Recording a Troubled World; What Does This-Have to Do with This?; When Can a Picture Save a Thousand Homes?; InSAR Studies of Alaska Volcanoes; Earth Observing-1 (EO-1) Data Products; Improving Access to the USGS Aerial Film Collections: High Resolution Scanners; Improving Access to the USGS Aerial Film Collections: Phoenix Digitizing System Product Distribution; System and Product Characterization: Issues Approach; Innovative Approaches to Analysis of Lidar Data for the National Map; Changes in Imperviousness near Military Installations; Geopositional Accuracy Evaluations of QuickBird and OrbView-3: Civil and Commercial Applications Project (CCAP); Geometric Accuracy Assessment: OrbView ORTHO Products; QuickBird Radiometric Calibration Update; OrbView-3 Radiometric Calibration; QuickBird Radiometric Characterization; NASA Radiometric Characterization; Establishing and Verifying the Traceability of Remote-Sensing Measurements to International Standards; QuickBird Applications; Airport Mapping and Perpetual Monitoring Using IKONOS; OrbView-3 Relative Accuracy Results and Impacts on Exploitation and Accuracy Improvement; Using Remotely Sensed Imagery to Determine Impervious Surface in Sioux Falls, South Dakota; Applying High-Resolution Satellite Imagery and Remotely Sensed Data to Local Government Applications: Sioux Falls, South Dakota; Automatic Co-Registration of QuickBird Data for Change Detection Applications; Developing Coastal Surface Roughness Maps Using ASTER and QuickBird Data Sources; Automated, Near-Real Time Cloud and Cloud Shadow Detection in High Resolution VNIR Imagery; Science Applications of High Resolution Imagery at the USGS EROS Data Center; Draft Plan for Characterizing Commercial Data Products in Support of Earth Science Research; Atmospheric Correction Prototype Algorithm for High Spatial Resolution Multispectral Earth Observing Imaging Systems; Determining Regional Arctic Tundra Carbon Exchange: A Bottom-Up Approach; Using IKONOS Imagery to Assess Impervious Surface Area, Riparian Buffers and Stream Health in the Mid-Atlantic Region; Commercial Remote Sensing Space Policy Civil Implementation Update; USGS Commercial Remote Sensing Data Contracts (CRSDC); and Commercial Remote Sensing Space Policy (CRSSP): Civil Near-Term Requirements Collection Update.

Small Satellites and the Nigerian National Space Programme

NASA Astrophysics Data System (ADS)

Borroffice, Robert; Chizea, Francis; Sun, Wei; Sweeting, Martin, , Sir

2002-01-01

Space technology and access to space have been elusive to most developing countries over the last half of the 21st century, which is attributed to very low par capital income and the lack of awareness of policy/decision makers about the role of space technology in national development. Space technology was seen as very expensive and prestigious, meant only for the major industrialized countries, while the developing countries should focus on building their national economy and providing food, shelter and other social amenities for their ever-growing populations. In the last decade, the trend has changed with many developing countries embracing spaced technology as one of the major ways of achieving sustainable development. The present trend towards the use of small satellites in meeting national needs has aided this transition because, apart from the small size, they are cheaper to build and to launch, with shorter development time, lower complexity, improved effectiveness and reduced operating costs. This in turn has made them more affordable and has opened up new avenues for the acquisition of satellite technology. The collaborative work between National Space Research and Development Agency of Nigeria (NASRDA) and Surrey Satellite and Technology Limited (SSTL) is a programme aimed at building two small satellites as a way of kick- starting the national space programme. The first project, NigeriaSAT-1, is an enhanced microsatellite carrying Earth observation payloads able to provide 32 metre GSD 3 band multispectral images with a 600km swath width. NigeriaSAT-1 is one of six microsatellites forming the Disaster Monitoring Constellation (DMC) alongside microsatellites contributed by Algeria, China, Turkey, Thailand and UK. Through participation in this international constellation, Nigeria will be able to receive images with a daily revisit worldwide. The EO images generated by NigeriaSAT-1 and the partner microsatellites will be used for providing rapid coverage of natural and man-made disasters but will also be used for monitoring rapidly changing and dynamic aspect of agriculture, the environment, pipeline oil spillages and other national remote sensing requirements. Commercial exploitation of this unique 24hr revisit EO data is planned to offset the government investment. The second national project is NigeriaSAT-2, which is a geostationary communications minisatellite that has been selected specifically to address the lack of communications infrastructure in Nigeria. Both NigeriaSAT-1 and NigeriaSAT-2 projects are being carried out in co-operation with the Surrey Space Centre (UK), combined with a detailed space know-how transfer and training to build up an indigenous Nigeria capability in space technology, EO and communications. While the acquisition and development space technology is the prime focus of the national space programme, an application center, education center and various space research centers are being formed to draw the maximum benefit of space activities for Nigeria. The paper will present the experience of Nigeria in examining the cost/benefit of an affordable space programme based upon small satellites with real applications that will benefit the people of the country. The Nigerian space policy and programme and its first two small satellite projects (NigeriaSAT-1/DMC and NigeriaSAT-2/GEMINI) will also be presented. This paper focuses on how a developing country can take advantage of a cheap and efficient means of gaining access to space and using space technology in achieving its socio-economic development plans.

Problems and Limitations of Satellite Image Orientation for Determination of Height Models

NASA Astrophysics Data System (ADS)

Jacobsen, K.

2017-05-01

The usual satellite image orientation is based on bias corrected rational polynomial coefficients (RPC). The RPC are describing the direct sensor orientation of the satellite images. The locations of the projection centres today are without problems, but an accuracy limit is caused by the attitudes. Very high resolution satellites today are very agile, able to change the pointed area over 200km within 10 to 11 seconds. The corresponding fast attitude acceleration of the satellite may cause a jitter which cannot be expressed by the third order RPC, even if it is recorded by the gyros. Only a correction of the image geometry may help, but usually this will not be done. The first indication of jitter problems is shown by systematic errors of the y-parallaxes (py) for the intersection of corresponding points during the computation of ground coordinates. These y-parallaxes have a limited influence to the ground coordinates, but similar problems can be expected for the x-parallaxes, determining directly the object height. Systematic y-parallaxes are shown for Ziyuan-3 (ZY3), WorldView-2 (WV2), Pleiades, Cartosat-1, IKONOS and GeoEye. Some of them have clear jitter effects. In addition linear trends of py can be seen. Linear trends in py and tilts in of computed height models may be caused by limited accuracy of the attitude registration, but also by bias correction with affinity transformation. The bias correction is based on ground control points (GCPs). The accuracy of the GCPs usually does not cause some limitations but the identification of the GCPs in the images may be difficult. With 2-dimensional bias corrected RPC-orientation by affinity transformation tilts of the generated height models may be caused, but due to large affine image deformations some satellites, as Cartosat-1, have to be handled with bias correction by affinity transformation. Instead of a 2-dimensional RPC-orientation also a 3-dimensional orientation is possible, respecting the object height more as by 2-dimensional orientation. The 3-dimensional orientation showed advantages for orientation based on a limited number of GCPs, but in case of poor GCP distribution it may cause also negative effects. For some of the used satellites the bias correction by affinity transformation showed advantages, but for some other the bias correction by shift was leading to a better levelling of the generated height models, even if the root mean square (RMS) differences at the GCPs were larger as for bias correction by affinity transformation. The generated height models can be analyzed and corrected with reference height models. For the used data sets accurate reference height models are available, but an analysis and correction with the free of charge available SRTM digital surface model (DSM) or ALOS World 3D (AW3D30) is also possible and leads to similar results. The comparison of the generated height models with the reference DSM shows some height undulations, but the major accuracy influence is caused by tilts of the height models. Some height model undulations reach up to 50 % of the ground sampling distance (GSD), this is not negligible but it cannot be seen not so much at the standard deviations of the height. In any case an improvement of the generated height models is possible with reference height models. If such corrections are applied it compensates possible negative effects of the type of bias correction or 2-dimensional orientations against 3-dimensional handling.

Generation and Characterization of Nanoaerosols Using a Portable Scanning Mobility Particle Sizer and Electron Microscopy

NASA Astrophysics Data System (ADS)

Marty, Adam J.

The purpose of this research is to demonstrate the ability to generate and characterize a nanometer sized aerosol using solutions, suspensions, and a bulk nanopowder, and to research the viability of using an acoustic dry aerosol generator/elutriator (ADAGE) to aerosolize a bulk nanopowder into a nanometer sized aerosol. The research compares the results from a portable scanning mobility particle sizer (SMPS) to the more traditional method of counting and sizing particles on a filter sample using scanning electron microscopy (SEM). Sodium chloride aerosol was used for the comparisons. The sputter coating thickness, a conductive coating necessary for SEM, was measured on different sizes of polystyrene latex spheres (PSLS). Aluminum oxide powder was aerosolized using an ADAGE and several different support membranes and sound frequency combinations were explored. A portable SMPS was used to determine the size distributions of the generated aerosols. Polycarbonate membrane (PCM) filter samples were collected for subsequent SEM analysis. The particle size distributions were determined from photographs of the membrane filters. SMPS data and membrane samples were collected simultaneously. The sputter coating thicknesses on four different sizes of PSLS, range 57 nanometers (nm) to 220 nm, were measured using transmission electron microscopy and the results from the SEM and SMPS were compared after accounting for the sputter coating thickness. Aluminum oxide nanopowder (20 nm) was aerosolized using a modified ADAGE technique. Four different support membranes and four different sound frequencies were tested with the ADAGE. The aerosol was collected onto PCM filters and the samples were examined using SEM. The results indicate that the SMPS and SEM distributions were log-normally distributed with a median diameter of approximately 42 nm and 55 nm, respectively, and geometric standard deviations (GSD) of approximately 1.6 and 1.7, respectively. The two methods yielded similar distributional trends with a difference in median diameters of approximately 11 -- 15 nm. The sputter coating thickness on the different sizes of PSLSs ranged from 15.4 -- 17.4 nm. The aerosols generated, using the modified ADAGE, were low in concentration. The particles remained as agglomerates and varied widely in size. An aluminum foil support membrane coupled with a high sound frequency generated the smallest agglomerates. A well characterized sodium chloride aerosol was generated and was reproducible. The distributions determined using SEM were slightly larger than those obtained from SMPS, however, the distributions had relatively the same shape as reflected in their GSDs. This suggests that a portable SMPS is a suitable method for characterizing a nanoaerosol. The sizing techniques could be compared after correcting for the effects of the sputter coating necessary for SEM examination. It was determined that the sputter coating thickness on nano-sized particles and particles up to approximately 220 nm can be expected to be the same and that the sputter coating can add considerably to the size of a nanoparticle. This has important implications for worker health where nanoaerosol exposure is a concern. The sputter coating must be considered when SEM is used to describe a nanoaerosol exposure. The performance of the modified ADAGE was less than expected. The low aerosol output from the ADAGE prevented a more detailed analysis and was limited to only a qualitative comparison. Some combinations of support membranes and sound frequencies performed better than others, particularly conductive support membranes and high sound frequencies. In conclusion, a portable SMPS yielded results similar to those obtained by SEM. The sputter coating was the same thickness on the PSLSs studied. The sputter coating thickness must be considered when characterizing nanoparticles using SEM. Finally, a conductive support membrane and higher frequencies appeared to generate the smallest agglomerates using the ADAGE technique.

Accuracy Assessment of Direct Georeferencing for Photogrammetric Applications on Small Unmanned Aerial Platforms

NASA Astrophysics Data System (ADS)

Mian, O.; Lutes, J.; Lipa, G.; Hutton, J. J.; Gavelle, E.; Borghini, S.

2016-03-01

Efficient mapping from unmanned aerial platforms cannot rely on aerial triangulation using known ground control points. The cost and time of setting ground control, added to the need for increased overlap between flight lines, severely limits the ability of small VTOL platforms, in particular, to handle mapping-grade missions of all but the very smallest survey areas. Applanix has brought its experience in manned photogrammetry applications to this challenge, setting out the requirements for increasing the efficiency of mapping operations from small UAVs, using survey-grade GNSS-Inertial technology to accomplish direct georeferencing of the platform and/or the imaging payload. The Direct Mapping Solution for Unmanned Aerial Vehicles (DMS-UAV) is a complete and ready-to-integrate OEM solution for Direct Georeferencing (DG) on unmanned aerial platforms. Designed as a solution for systems integrators to create mapping payloads for UAVs of all types and sizes, the DMS produces directly georeferenced products for any imaging payload (visual, LiDAR, infrared, multispectral imaging, even video). Additionally, DMS addresses the airframe's requirements for high-accuracy position and orientation for such tasks as precision RTK landing and Precision Orientation for Air Data Systems (ADS), Guidance and Control. This paper presents results using a DMS comprised of an Applanix APX-15 UAV with a Sony a7R camera to produce highly accurate orthorectified imagery without Ground Control Points on a Microdrones md4-1000 platform conducted by Applanix and Avyon. APX-15 UAV is a single-board, small-form-factor GNSS-Inertial system designed for use on small, lightweight platforms. The Sony a7R is a prosumer digital RGB camera sensor, with a 36MP, 4.9-micron CCD producing images at 7360 columns by 4912 rows. It was configured with a 50mm AF-S Nikkor f/1.8 lens and subsequently with a 35mm Zeiss Sonnar T* FE F2.8 lens. Both the camera/lens combinations and the APX-15 were mounted to a Microdrones md4-1000 quad-rotor VTOL UAV. The Sony A7R and each lens combination were focused and calibrated terrestrially using the Applanix camera calibration facility, and then integrated with the APX-15 GNSS-Inertial system using a custom mount specifically designed for UAV applications. The mount is constructed in such a way as to maintain the stability of both the interior orientation and IMU boresight calibration over shock and vibration, thus turning the Sony A7R into a metric imaging solution. In July and August 2015, Applanix and Avyon carried out a series of test flights of this system. The goal of these test flights was to assess the performance of DMS APX-15 direct georeferencing system under various scenarios. Furthermore, an examination of how DMS APX-15 can be used to produce accurate map products without the use of ground control points and with reduced sidelap was also carried out. Reducing the side lap for survey missions performed by small UAVs can significantly increase the mapping productivity of these platforms. The area mapped during the first flight campaign was a 250m x 300m block and a 775m long railway corridor in a rural setting in Ontario, Canada. The second area mapped was a 450m long corridor over a dam known as Fryer Dam (over Richelieu River in Quebec, Canada). Several ground control points were distributed within both test areas. The flight over the block area included 8 North-South lines and 1 cross strip flown at 80m AGL, resulting in a ~1cm GSD. The flight over the railway corridor included 2 North-South lines also flown at 80m AGL. Similarly, the flight over the dam corridor included 2 North-South lines flown at 50m AGL. The focus of this paper was to analyse the results obtained from the two corridors. Test results from both areas were processed using Direct Georeferencing techniques, and then compared for accuracy against the known positions of ground control points in each test area. The GNSS-Inertial data collected by the APX-15 was post-processed in Single Base mode, using a base station located in the project area via POSPac UAV. For the block and railway corridor, the basestation's position was precisely determined by processing a 12-hour session using the CSRS-PPP Post Processing service. Similarly, for the flight over Fryer Dam, the base-station's position was also precisely determined by processing a 4-hour session using the CSRS-PPP Post Processing service. POSPac UAV's camera calibration and quality control (CalQC) module was used to refine the camera interior orientation parameters using an Integrated Sensor Orientation (ISO) approach. POSPac UAV was also used to generate the Exterior Orientation parameters for images collected during the test flight. The Inpho photogrammetric software package was used to develop the final map products for both corridors under various scenarios. The imagery was first imported into an Inpho project, with updated focal length, principal point offsets and Exterior Orientation parameters. First, a Digital Terrain/Surface Model (DTM/DSM) was extracted from the stereo imagery, following which the raw images were orthorectified to produce an orthomosaic product.