Stability analysis of hybrid-driven underwater glider

NASA Astrophysics Data System (ADS)

Niu, Wen-dong; Wang, Shu-xin; Wang, Yan-hui; Song, Yang; Zhu, Ya-qiang

2017-10-01

Hybrid-driven underwater glider is a new type of unmanned underwater vehicle, which combines the advantages of autonomous underwater vehicles and traditional underwater gliders. The autonomous underwater vehicles have good maneuverability and can travel with a high speed, while the traditional underwater gliders are highlighted by low power consumption, long voyage, long endurance and good stealth characteristics. The hybrid-driven underwater gliders can realize variable motion profiles by their own buoyancy-driven and propeller propulsion systems. Stability of the mechanical system determines the performance of the system. In this paper, the Petrel-II hybrid-driven underwater glider developed by Tianjin University is selected as the research object and the stability of hybrid-driven underwater glider unitedly controlled by buoyancy and propeller has been targeted and evidenced. The dimensionless equations of the hybrid-driven underwater glider are obtained when the propeller is working. Then, the steady speed and steady glide path angle under steady-state motion have also been achieved. The steady-state operating conditions can be calculated when the hybrid-driven underwater glider reaches the desired steady-state motion. And the steadystate operating conditions are relatively conservative at the lower bound of the velocity range compared with the range of the velocity derived from the method of the composite Lyapunov function. By calculating the hydrodynamic coefficients of the Petrel-II hybrid-driven underwater glider, the simulation analysis has been conducted. In addition, the results of the field trials conducted in the South China Sea and the Danjiangkou Reservoir of China have been presented to illustrate the validity of the analysis and simulation, and to show the feasibility of the method of the composite Lyapunov function which verifies the stability of the Petrel-II hybrid-driven underwater glider.

Experimental study of the flight envelope and research of safety requirements for hang-gliders

NASA Technical Reports Server (NTRS)

Laburthe, C.

1979-01-01

The flight mechanic computations were computed, providing both the flight envelopes with all sorts of limits and a fairly precise idea of the influence of several parameters, such as pilot's weight, wing settings, aeroelasticity, etc... The particular problem of luffing dives was thoroughly analyzed, and two kinds of causes were exhibited in both the rules of luffing and aeroelastic effects. The general analysis of longitudinal stability showed a strong link with fabric tension, as expected through Nielsen's and Twaites' theory. Fabric tension strongly depending upon aeroelasticity, that parameter was found to be the most effective design one for positive stability. Lateral stability was found to be very similar in all gliders except perhaps the cylindro-conical. The loss of stability happens in roll at low angle of attack, whereas it happens in yaw at high angle. Turning performance was a bit suprising, with a common maximum value of approximately 55 deg of bank angle for a steady turn.

An Expansion of Glider Observation STrategies to Systematically Transmit and Analyze Preferred Waypoints of Underwater Gliders

DTIC Science & Technology

2015-01-01

exercis ead over time i e rendezvous C function. nformation ne that set of po antify the relat are combined to as a morpho runs independ rs. EMPath a...Glider pilots do not use the temporal waypoint estimate from GOST in their control of the glider; waypoints are simply treated as a sequence of points...glider pilots’ interpretation of points as only sequential instead of temporal will eliminate this as a hindrance to the system’s use. Further

Glider Observations of Circulation Around an Island

DTIC Science & Technology

2015-09-30

1 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. Glider Observations of Circulation Around an Island...of island circulation through observations using underwater gliders , with the ultimate goal of better prediction. OBJECTIVES Given a goal of...APPROACH We are making Spray glider observations from Palau to resolve the phenomena described above. We have extensive experience deploying

Glider kits cut truck ownership costs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smiley, C.H.

1983-06-01

A glider kit consists of a new truck without the power train, with the customer supplying the engine, transmission, clutch, driveline and rear axles for mounting in a new frame and cab complete with steering axle, wiring harnesses, exhaust system and cooling system. Glider kits have long been regarded as a cost effective means of replacing accident or fire damaged late model vehicles. The financial advantages, assembly and remanufacturing are discussed. Points out that at a basic cost about one third of a new truck, a glider kit offers lower cost of borrowed capital, tax savings, and elimination of highmore » maintenance and downtime costs. The paper concludes that use of glider kits as an alternative to major repair or reconditioning or outright replacement of coal haulers presents some interesting options.« less

SOCIB Glider toolbox: from sensor to data repository

NASA Astrophysics Data System (ADS)

Pau Beltran, Joan; Heslop, Emma; Ruiz, Simón; Troupin, Charles; Tintoré, Joaquín

2015-04-01

Nowadays in oceanography, gliders constitutes a mature, cost-effective technology for the acquisition of measurements independently of the sea state (unlike ships), providing subsurface data during sustained periods, including extreme weather events. The SOCIB glider toolbox is a set of MATLAB/Octave scripts and functions developed in order to manage the data collected by a glider fleet. They cover the main stages of the data management process, both in real-time and delayed-time modes: metadata aggregation, downloading, processing, and automatic generation of data products and figures. The toolbox is distributed under the GNU licence (http://www.gnu.org/copyleft/gpl.html) and is available at http://www.socib.es/users/glider/glider_toolbox.

Hang cleans and hang snatches produce similar improvements in female collegiate athletes

PubMed Central

Ayers, JL; DeBeliso, M; Sevene, TG

2016-01-01

Olympic weightlifting movements and their variations are believed to be among the most effective ways to improve power, strength, and speed in athletes. This study investigated the effects of two Olympic weightlifting variations (hang cleans and hang snatches), on power (vertical jump height), strength (1RM back squat), and speed (40-yard sprint) in female collegiate athletes. 23 NCAA Division I female athletes were randomly assigned to either a hang clean group or hang snatch group. Athletes participated in two workout sessions a week for six weeks, performing either hang cleans or hang snatches for five sets of three repetitions with a load of 80-85% 1RM, concurrent with their existing, season-specific, resistance training program. Vertical jump height, 1RM back squat, and 40-yard sprint all had a significant, positive improvement from pre-training to post-training in both groups (p≤0.01). However, when comparing the gain scores between groups, there was no significant difference between the hang clean and hang snatch groups for any of the three dependent variables (i.e., vertical jump height, p=0.46; 1RM back squat, p=0.20; and 40-yard sprint, p=0.46). Short-term training emphasizing hang cleans or hang snatches produced similar improvements in power, strength, and speed in female collegiate athletes. This provides strength and conditioning professionals with two viable programmatic options in athletic-based exercises to improve power, strength, and speed. PMID:27601779

Hang cleans and hang snatches produce similar improvements in female collegiate athletes.

PubMed

Ayers, J L; DeBeliso, M; Sevene, T G; Adams, K J

2016-09-01

Olympic weightlifting movements and their variations are believed to be among the most effective ways to improve power, strength, and speed in athletes. This study investigated the effects of two Olympic weightlifting variations (hang cleans and hang snatches), on power (vertical jump height), strength (1RM back squat), and speed (40-yard sprint) in female collegiate athletes. 23 NCAA Division I female athletes were randomly assigned to either a hang clean group or hang snatch group. Athletes participated in two workout sessions a week for six weeks, performing either hang cleans or hang snatches for five sets of three repetitions with a load of 80-85% 1RM, concurrent with their existing, season-specific, resistance training program. Vertical jump height, 1RM back squat, and 40-yard sprint all had a significant, positive improvement from pre-training to post-training in both groups (p≤0.01). However, when comparing the gain scores between groups, there was no significant difference between the hang clean and hang snatch groups for any of the three dependent variables (i.e., vertical jump height, p=0.46; 1RM back squat, p=0.20; and 40-yard sprint, p=0.46). Short-term training emphasizing hang cleans or hang snatches produced similar improvements in power, strength, and speed in female collegiate athletes. This provides strength and conditioning professionals with two viable programmatic options in athletic-based exercises to improve power, strength, and speed.

The design of high-performance gliders

NASA Technical Reports Server (NTRS)

Mueller, B.; Heuermann, V.

1985-01-01

A high-performance glider is defined as a glider which has been designed to carry the pilot in a minimum of time a given distance, taking into account conditions which are as conveniently as possible. The present investigation has the objective to show approaches for enhancing the cross-country flight cruising speed, giving attention to the difficulties which the design engineer will have to overcome. The characteristics of the cross-country flight and their relation to the cruising speed are discussed, and a description is provided of mathematical expressions concerning the cruising speed, the sinking speed, and the optimum gliding speed. The effect of aspect ratio and wing loading on the cruising speed is illustrated with the aid of a graph. Trends in glider development are explored, taking into consideration the design of laminar profiles, the reduction of profile-related drag by plain flaps, and the variation of wing loading during the flight. A number of suggestions are made for obtaining gliders with improved performance.

Glider communications and controls for the sea sentry mission.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feddema, John Todd; Dohner, Jeffrey Lynn

2005-03-01

This report describes a system level study on the use of a swarm of sea gliders to detect, confirm and kill littoral submarine threats. The report begins with a description of the problem and derives the probability of detecting a constant speed threat without networking. It was concluded that glider motion does little to improve this probability unless the speed of a glider is greater than the speed of the threat. Therefore, before detection, the optimal character for a swarm of gliders is simply to lie in wait for the detection of a threat. The report proceeds by describing themore » effect of noise on the localization of a threat once initial detection is achieved. This noise is estimated as a function of threat location relative to the glider and is temporally reduced through the use of an information or Kalman filtering. In the next section, the swarm probability of confirming and killing a threat is formulated. Results are compared to a collection of stationary sensors. These results show that once a glider has the ability to move faster than the threat, the performance of the swarm is equal to the performance of a stationary swarm of gliders with confirmation and kill ranges equal to detection range. Moreover, at glider speeds greater than the speed of the threat, swarm performance becomes a weak function of speed. At these speeds swarm performance is dominated by detection range. Therefore, to future enhance swarm performance or to reduce the number of gliders required for a given performance, detection range must be increased. Communications latency is also examined. It was found that relatively large communication delays did little to change swarm performance. Thus gliders may come to the surface and use SATCOMS to effectively communicate in this application.« less

Flight Instructor Practical Test Standards for Glider

DOT National Transportation Integrated Search

1997-10-01

This Flight Instructor-Glider Practical Test Standards book has been : published by the Federal Aviation Administration to establish the : standards for the flight instructor certification practical tests for the : glider category. FAA inspectors and...

Wind tunnel tests of four flexible wing ultralight gliders

NASA Technical Reports Server (NTRS)

Ormiston, R. A.

1979-01-01

The aerodynamic lift, drag, and pitching moment characteristics of four full scale, flexible wing, ultralight gliders were measured in the settling chamber of a low speed wind tunnel. The gliders were tested over a wide range of angle of attack and at two different velocities. Particular attention was devoted to the lift and pitching moment behavior at low and negative angles of attack because of the potential loss of longitudinal stability of flexible wing gliders in this regime. The test results were used to estimate the performance and longitudinal control characteristics of the gliders.

Glider accidents: an analysis of 143 cases, 2001-2005.

PubMed

van Doorn, Robert R A; de Voogt, Alexander J

2007-01-01

The majority of aviation crashes and casualties take place in general and sport aviation. Although gliding has gained popularity in recent decades, we could find no systematic analysis of glider accidents. This study determined factors associated with both non-fatal and fatal glider accidents to document their position within sport and general aviation accidents, and to suggest preventive measures and improvements. We performed a retrospective review of glider accidents for the period 2001-2005 in the database maintained by the U.S. National Transportation Safety Board (NTSB). A total of 117 non-fatal and 26 fatal glider accidents were reported for the 5-yr period. Adverse weather was the cause in 20% of all non-fatal accidents, 60% of which occurred in the cruise phase. Logistic regression revealed that fatal accidents were predicted by pilot error, flight phase, and home-built aircraft. Factors contributing to glider crashes are specific to this type of sport aviation. Owners of home-built gliders should pay particular attention to the aircraft's specifications and design limits.

Underwater glider observations of the ongoing El Niño

NASA Astrophysics Data System (ADS)

Rudnick, D. L.; Owens, B.; Johnston, S.; Karnauskas, K.

2016-02-01

We report on observations by underwater gliders in the equatorial current system along 93°W and 95°W between 2°S and 2°N starting in October 2013 and continuing through the present. The project Repeat Observations by Gliders in the Equatorial Region (ROGER) was conceived with the intention of using underwater gliders to make repeat sections across equatorial system to quantify the location and strength of the Equatorial Undercurrent (EUC) and the equatorial front. ROGER serendipitously started near the beginning of a series of events that have led to the El Niño currently ongoing. We use Spray underwater gliders equipped with CTDs and ADCPs to measure pressure, temperature, salinity, velocity and chlorophyll fluorescence in a series of deployments from the Galapagos Islands. At the time of writing of this abstract, we have completed 15 glider missions, with 3 currently underway. Gliders have completed 7300 dives to as deep as 1000 m, traveling 27,000 km in 1600 glider-days. To our knowledge, this is the most extensive glider data set ever collected in the equatorial current system. With 6-km horizontal spacing between profiles, these more than 30 sections across the equator allow a finely-resolved look at the passage of Kelvin waves that establish El Niño. The Kelvin waves are manifest as deepening of the thermocline, warming of the surface, strengthening of the EUC, and northward migration of the equatorial front. We will present an up-to-date account of the continuing glider observations of El Niño.

Glider Observations of Upper Ocean Structure in the Bay of Bengal

DTIC Science & Technology

2015-09-30

1 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. Glider Observations of Upper Ocean Structure in the Bay...using gliders and floats • Improve glider technology to overcome fresh, buoyant surface layers • Establish a new technology to observe turbulence...with profiling floats APPROACH We use two approaches to observe the upper ocean in the BoB. First, we deploy Spray underwater gliders to resolve

14 CFR 91.309 - Towing: Gliders and unpowered ultralight vehicles.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Towing: Gliders and unpowered ultralight... Flight Operations § 91.309 Towing: Gliders and unpowered ultralight vehicles. (a) No person may operate a civil aircraft towing a glider or unpowered ultralight vehicle unless— (1) The pilot in command of the...

14 CFR 91.309 - Towing: Gliders and unpowered ultralight vehicles.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Towing: Gliders and unpowered ultralight... Flight Operations § 91.309 Towing: Gliders and unpowered ultralight vehicles. (a) No person may operate a civil aircraft towing a glider or unpowered ultralight vehicle unless— (1) The pilot in command of the...

14 CFR 91.309 - Towing: Gliders and unpowered ultralight vehicles.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Towing: Gliders and unpowered ultralight... Flight Operations § 91.309 Towing: Gliders and unpowered ultralight vehicles. (a) No person may operate a civil aircraft towing a glider or unpowered ultralight vehicle unless— (1) The pilot in command of the...

14 CFR 91.309 - Towing: Gliders and unpowered ultralight vehicles.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Towing: Gliders and unpowered ultralight... Flight Operations § 91.309 Towing: Gliders and unpowered ultralight vehicles. (a) No person may operate a civil aircraft towing a glider or unpowered ultralight vehicle unless— (1) The pilot in command of the...

Optimal trajectory planning for a UAV glider using atmospheric thermals

NASA Astrophysics Data System (ADS)

Kagabo, Wilson B.

An Unmanned Aerial Vehicle Glider (UAV glider) uses atmospheric energy in its different forms to remain aloft for extended flight durations. This UAV glider's aim is to extract atmospheric thermal energy and use it to supplement its battery energy usage and increase the mission period. Given an infrared camera identified atmospheric thermal of known strength and location; current wind speed and direction; current battery level; altitude and location of the UAV glider; and estimating the expected altitude gain from the thermal, is it possible to make an energy-efficient based motivation to fly to an atmospheric thermal so as to achieve UAV glider extended flight time? For this work, an infrared thermal camera aboard the UAV glider takes continuous forward-looking ground images of "hot spots". Through image processing a candidate atmospheric thermal strength and location is estimated. An Intelligent Decision Model incorporates this information with the current UAV glider status and weather conditions to provide an energy-based recommendation to modify the flight path of the UAV glider. Research, development, and simulation of the Intelligent Decision Model is the primary focus of this work. Three models are developed: (1) Battery Usage Model, (2) Intelligent Decision Model, and (3) Altitude Gain Model. The Battery Usage Model comes from the candidate flight trajectory, wind speed & direction and aircraft dynamic model. Intelligent Decision Model uses a fuzzy logic based approach. The Altitude Gain Model requires the strength and size of the thermal and is found a priori.

Two Simple Tricks with Air-Rail Gliders

ERIC Educational Resources Information Center

Barnes, George

1974-01-01

Describes a technique for imparting essentially the same speed, in opposite directions, to two gliders to demonstrate that total momentum of equal masses traveling at the same speed in opposite directions is zero. Also describes a technique using a compression spring and beeswax for momentarily holding two gliders together prior to starting them…

Implementation of glider guns in the light-sensitive Belousov-Zhabotinsky medium.

PubMed

de Lacy Costello, Ben; Toth, Rita; Stone, Christopher; Adamatzky, Andrew; Bull, Larry

2009-02-01

In cellular automata models a glider gun is an oscillating pattern of nonquiescent states that periodically emits traveling localizations (gliders). The glider streams can be combined to construct functionally complete systems of logical gates and thus realize universal computation. The glider gun is the only means of ensuring the negation operation without additional external input and therefore is an essential component of a collision-based computing circuit. We demonstrate the existence of glider-gun-like structures in both experimental and numerical studies of an excitable chemical system-the light-sensitive Belousov-Zhabotinsky reaction. These discoveries could provide the basis for future designs of collision-based reaction-diffusion computers.

Multi-Scale 4DVAR Assimilation of Glider Teams on the North Carolina Shelf

NASA Astrophysics Data System (ADS)

Osborne, J. J. V.; Carrier, M.; Book, J. W.; Barron, C. N.; Rice, A. E.; Rowley, C. D.; Smedstad, L.; Souopgui, I.; Teague, W. J.

2017-12-01

We demonstrate a method to assimilate glider profile data from multiple gliders in close proximity ( 10 km or less). Gliders were deployed in a field experiment from 17 May until 4 June 2017, north of Cape Hatteras and inshore of the Gulf Stream. Gliders were divided into two teams, generally two or three gliders per team. One team was tasked with station keeping and the other with moving and sampling regions of high variability in temperature and salinity. Glider data are assimilated into the Relocatable Navy Coastal Ocean Model (RELO NCOM) with four dimensional variational assimilation (NCOM-4DVAR). RELO NCOM is used by the US Navy to predict the ocean. RELO NCOM is a baroclinic, Boussinesq, free-surface, and hydrostatic ocean model with a flexible sigma-z vertical coordinate. Two domains are used, one focused north and one focused south of Cape Hatteras. The domains overlap near the gliders, thus providing two forecasts near the gliders. Both domains have 1 km horizontal resolution. Data are assimilated in a newly-developed multi-scale data-processing and assimilating approach using NCOM-4DVAR. This enables NCOM-4DVAR to use many more observations than standard NCOM-4DVAR, improving the analysis and forecast. Assimilation experiments use station-keeping glider data, moving glider data, or all glider data. Sea surface temperature (SST) data and satellite altimeter (SSH) data are also assimilated. An additional experiment omits glider data but still assimilates SST and SSH data. Conductivity, temperature, and depth (CTD) profiles from the R/V Savannah are used for validation, including data from an underway CTD (UCTD). Data from glider teams have the potential to significantly improve model forecasts. Missions using teams of gliders can be planned to maximize data assimilation for optimal impact on model predictions.

Aerodynamic Performance of Hand Launch Glider

NASA Astrophysics Data System (ADS)

Koike, Masaru; Ishii, Mitsuru

In recent years Micro Air Vehicles (MAV) for disaster aerial video are developed vigorously. In order to improve aerodynamic performance of MAV wing performance in low Reynolds numbers (Re) need to be improved, but research on the theme are very rare. In category of Hand Launch Glider, a kind of model aircraft, glide performance are competed, as a result high performance airfoils in Re is around 20,000 are developed. Therefore for MAV's aerodynamic performance improvement airfoils of Hand Launch Gliders should be referred and aerodynamic characteristics of the airfoils desired to be studied. So in this research, aerodynamic characteristics of the gliders are measured in wind tunnel. And also consistency between wind tunnel test and glide test in calm air is examined to confirm reliability of wind tunnel test. Comparison of different airfoils and flow visualization are also performed.

The cognitive domain of a glider in the game of life.

PubMed

Beer, Randall D

2014-01-01

This article examines in some technical detail the application of Maturana and Varela's biology of cognition to a simple concrete model: a glider in the game of Life cellular automaton. By adopting an autopoietic perspective on a glider, the set of possible perturbations to it can be divided into destructive and nondestructive subsets. From a glider's reaction to each nondestructive perturbation, its cognitive domain is then mapped. In addition, the structure of a glider's possible knowledge of its immediate environment, and the way in which that knowledge is grounded in its constitution, are fully described. The notion of structural coupling is then explored by characterizing the paths of mutual perturbation that a glider and its environment can undergo. Finally, a simple example of a communicative interaction between two gliders is given. The article concludes with a discussion of the potential implications of this analysis for the enactive approach to cognition.

Glider Observations of Internal Tide Packets on the Australian Northwest Shelf

NASA Astrophysics Data System (ADS)

Book, J. W.; Steinberg, C. R.; Brinkman, R. M.; Jones, N. L.; Lowe, R.; Ivey, G. N.; Pattiaratchi, C. B.; Rice, A. E.

2016-02-01

The rapid profiling capabilities (less than 10 minutes per profile in 100 m of water excluding surfacing times) of autonomous gliders were utilized to study the structure of non-linear internal tide packets on the Australian Northwest Shelf. A total of five gliders were deployed on the shelf from 11 February - 21 April 2012 with more than 2900 glider CTD profiles collected during the final three weeks of this time period when the internal tide activity was intense. In general the internal tide packets showed high degrees of non-linearity, for example in one case a glider observed a 62 m rise of the 28° isotherm over 2.25 hours in a shelf location of 90 meters water depth. In addition to the glider measurements, moored strings of CTD sensors were used to measure the internal tide packets at fixed positions and the results show that the wave packets vary significantly with respect to their structure and arrival times from one tidal period to the next. This fact complicates interpretation of the glider data as wave packet spatial evolution is non-stationary and cannot be simply recovered from repeat glider visits to the same location. Furthermore, the packets were found to move at speeds near or greater (e.g., 0.55 m/s) than the speed that the gliders were moving. Despite these challenges, the gliders offer the only resource that can measure the spatial structure of the wave packets beyond the scope of our limited mooring positions. Therefore, we have implemented methods such as time-augmented empirical orthogonal functions to combine these glider measurements with the fixed mooring measurements in order to better understand the spatial and temporal patterns of the wave packet evolution over the slope and shelf of this region.

Measuring sea surface height with a GNSS-Wave Glider

NASA Astrophysics Data System (ADS)

Morales Maqueda, Miguel Angel; Penna, Nigel T.; Foden, Peter R.; Martin, Ian; Cipollini, Paolo; Williams, Simon D.; Pugh, Jeff P.

2017-04-01

A GNSS-Wave Glider is a novel technique to measure sea surface height autonomously using the Global Navigation Satellite System (GNSS). It consists of an unmanned surface vehicle manufactured by Liquid Robotics, a Wave Glider, and a geodetic-grade GNSS antenna-receiver system, with the antenna installed on a mast on the vehicle's deck. The Wave Glider uses the differential wave motion through the water column for propulsion, thus guaranteeing an, in principle, indefinite autonomy. Solar energy is collected to power all on-board instrumentation, including the GNSS system. The GNSS-Wave Glider was first tested in Loch Ness in 2013, demonstrating that the technology is capable of mapping geoid heights within the loch with an accuracy of a few centimetres. The trial in Loch Ness did not conclusively confirm the reliability of the technique because, during the tests, the state of the water surface was much more benign than would normally be expect in the open ocean. We now report on a first deployment of a GNSS-Wave Glider in the North Sea. The deployment took place in August 2016 and lasted thirteen days, during which the vehicle covered a distance of about 350 nautical miles in the north western North Sea off Great Britain. During the experiment, the GNSS-Wave Glider experienced sea states between 1 (0-0.1 m wave heights) and 5 (2.5-4 m wave heights). The GNSS-Wave Glider data, recorded at 5 Hz frequency, were analysed using a post-processed kinematic GPS-GLONASS precise point positioning (PPP) approach, which were quality controlled using double difference GPS kinematic processing with respect to onshore reference stations. Filtered with a 900 s moving-average window, the PPP heights reveal geoid patterns in the survey area that are very similar to the EGM2008 geoid model, thus demonstrating the potential use of a GNSS-Wave Glider for marine geoid determination. The residual of subtracting the modelled or measured marine geoid from the PPP signal combines information

Slocum-TREC Thermal Glider

NASA Technical Reports Server (NTRS)

Huntsberger, Terry; Jones, Jack A.; Valdez, Thomas; Stirbl, Rob

2012-01-01

JPL is now teaming with Teledyne-Webb-Research to produce the first thermal glider that uses PCM for direct buoyancy control (not electronic pumps) and to produce all other required electricity. TWR has increased electrical production total efficiency from 0.45 to 0.61. JPL performed over 6000 cycles (2.4 years) of accelerated life testing of a piston accumulator. Design, fabrication, and testing will be performed in 2012, with deployment by Rutgers University in late 2012. The proposed goal of the ocean endurance tests was 3 months, but will be extended to at last 1 year. The piston accumulator was cycled over 6,000 times in a three-month period in order to simulate continuous glider life of about 2.4 years, The life test data suggests that we might accumulate roughly 0.5 cc of gas in the oil bladders for each cycle. PCM canisters will be fabricated with a compressed aluminum foam core, 7.5% foam selected (< 42 minutes to freeze anticipated) An Axi 5345/18 3-Phase AC alternator selected for power generation, 61% energy storage efficiency A 4-Cell A123 Energy Storage Systems battery selected for energy storage, buss voltage 14.4 to 12.5 V (13.2 V nominal) Glider deployment expected in late 2012

Calibration procedure for Slocum glider deployed optical instruments.

PubMed

Cetinić, Ivona; Toro-Farmer, Gerardo; Ragan, Matthew; Oberg, Carl; Jones, Burton H

2009-08-31

Recent developments in the field of the autonomous underwater vehicles allow the wide usage of these platforms as part of scientific experiments, monitoring campaigns and more. The vehicles are often equipped with sensors measuring temperature, conductivity, chlorophyll a fluorescence (Chl a), colored dissolved organic matter (CDOM) fluorescence, phycoerithrin (PE) fluorescence and spectral volume scattering function at 117 degrees, providing users with high resolution, real time data. However, calibration of these instruments can be problematic. Most in situ calibrations are performed by deploying complementary instrument packages or water samplers in the proximity of the glider. Laboratory calibrations of the mounted sensors are difficult due to the placement of the instruments within the body of the vehicle. For the laboratory calibrations of the Slocum glider instruments we developed a small calibration chamber where we can perform precise calibrations of the optical instruments aboard our glider, as well as sensors from other deployment platforms. These procedures enable us to obtain pre- and post-deployment calibrations for optical fluorescence instruments, which may differ due to the biofouling and other physical damage that can occur during long-term glider deployments. We found that biofouling caused significant changes in the calibration scaling factors of fluorescent sensors, suggesting the need for consistent and repetitive calibrations for gliders as proposed in this paper.

The Glider registry.

PubMed

Secco, Gioel Gabrio; Rittger, Harald; Hoffmann, Stefan; Richardt, Gert; Abdel-Wahab, Mohamed; Reinecke, Holger; Lotan, Chaim; Werner, Gerald; Sievert, Horst; Foin, Nicolas; Di Mario, Carlo

2017-01-01

Provisional stenting of the side-branch (SB) is the universally accepted gold standard while there is still controversy on the usefulness of routine dilatation of the SB ostium. Recrossing the struts of a previously deployed stent with a wire and a balloon can prove challenging and is occasionally unsuccessful, mainly because the balloon tip hits a stent strut. This prospective multicenter international registry tested the crossing ability procedural results of a new-dedicated ultrashort balloon specifically designed for side branch dilatation (Glider, TriReme Medical, Pleasanton, CA, USA). One hundred and twenty five patients (for a total of 131 bifurcation lesions) were enrolled in the registry between January 2009 and May 2012. The Glider was used as first choice in alternative to conventional balloon (group I, 72%) or as bail-out after unsuccessful previous attempt at crossing with small conventional low-profile balloons (group II, 28%). Postprocedural coronary artery dissections and in-hospital MACE (death, myocardial infarction and repeat revascularization) were assessed. Technical success was defined as the ability of the Glider to recross the struts of a previously deployed stent while procedural success was defined as less of residual 50% diameter stenosis at the origin of the SB with a final TIMI 3 and/or freedom from in-hospital MACE. Technical success was achieved, respectively, in 92% (group I), and 83% (group II). Clinical and angiographic procedural success was achieved in 98% of the lesions. In Group II, no other balloon of the same size could cross in cases where Glider could not. A total of 13 complications were observed, including nine ostial SB dissection four of which needed a second stent on the SB, one stent loss, two severe coronary spasms, and two by thrombus formation. The unique possibility offered by this short dedicated balloon to orientate its beveled tip provides an effective strategy for recrossing stent struts when conventional low

Effect of Aerodynamic Design on Glider Performance

NASA Technical Reports Server (NTRS)

Lippisch, A

1935-01-01

The performance of a glider is determined by means of the velocity polar, which represents the connection between horizontal and sinking speed. The mean sinking speed for a given speed range can be determined on the basis of the velocity polar. These data form the basis for the most propitious design of a performance-type glider with a view to long-distance flight.

Hypersonic Boost Glider

NASA Image and Video Library

1957-04-15

Hypersonic Boost Glider in 11 Inch Hypersonic Tunnel L57-1681 In 1957 Langley tested its HYWARDS design in the 11 Inch Hypersonic Tunnel. Photograph published in Engineer in Charge: A History of the Langley Aeronautical Laboratory, 1917-1958 by James R. Hansen. Page 369.

The flight of a balsa glider

NASA Astrophysics Data System (ADS)

Waltham, Chris

1999-07-01

A simple analysis is performed on the flight of a small balsa toy glider. All the basic features of flight have to be included in the calculation. Key differences between the flight of small objects like the glider, and full-sized aircraft, are examined. Good agreement with experimental data is obtained when only one parameter, the drag coefficient, is allowed to vary. The experimental drag coefficient is found to be within a factor of 2 of that obtained using the theory of ideal flat plates.

Evaluating Lake Superior nearshore offshore gradients using autonomous gliders

EPA Science Inventory

Slocum electric gliders are autonomous vehicles capable of continuously mapping subsurface conditions at high resolution for months at a time. During the 2016 CSMI in Lake Superior, seven glider deployments were undertaken through a partnership between University of Minnesota Dul...

Marginal Ice Zone: Biogeochemical Sampling with Gliders

DTIC Science & Technology

2014-09-30

chlorophyll primary productivity model to estimate and compare phytoplankton productivity under full ice cover, in the MIZ, and in open ice-free water...September, the gliders and still operating but will be retrieved in early October from the R/V Norseman. All gliders carried sensors for chlorophyll ...program, with modification for local conditions. The specific protocols for each sensor – backscatter and chlorophyll fluorescence – are described in

Air Transport by Gliders : Some Technical Observations

NASA Technical Reports Server (NTRS)

Stepniewski, Wieslaw

1941-01-01

This short analysis may be useful in determining the real tactical possibilities of "glider trains" and in adopting the course to be followed in possible studies of these questions. In this analysis most prominent are: (a) the power required for the train in level flight; (b) its speed; (c) climb; and (d) the type of airplane best suited for towing as well as design requirements for transport gliders.

Upper-stratospheric glider flights for low-g experimentation

NASA Astrophysics Data System (ADS)

Loesch, Adam

Near Space Corporation's fully-operational High Altitude Shuttle System (HASS) consists of a glider carried to 100,000ft by a high altitude balloon. Originally intended to safely return sensitive instrumentation from altitude back to Earth, the glider provides the opportunity to fly ultra-smooth "parabolas" for low-g experimentation. This work models the dynamic behavior of the glider using aerodynamic parameters of a scaled F-4 Phantom to determine the optimal flight path during descent. Low-g parabola and pull-up pairs are flown until the altitude drops below 18km, approaching the maximum altitude of controlled airspace. With this model, it was found that eleven low-g parabolas can be flown to yield 137 seconds of total test time at an average RMS g-loading of 4.9x10 -2. By changing the weighting factor of the merit function, a tradeoff can be made to increase total test time at the expense of increasing g-loading, or vice-versa. A preliminary design exercise for an improved glider is conducted based on lessons learned from the scaled F-4 flight results.

Biogeochemistry from Gliders at the Hawaii Ocean Times-Series

NASA Astrophysics Data System (ADS)

Nicholson, D. P.; Barone, B.; Karl, D. M.

2016-02-01

At the Hawaii Ocean Time-series (HOT) autonomous, underwater gliders equipped with biogeochemical sensors observe the oceans for months at a time, sampling spatiotemporal scales missed by the ship-based programs. Over the last decade, glider data augmented by a foundation of time-series observations have shed light on biogeochemical dynamics occuring spatially at meso- and submesoscales and temporally on scales from diel to annual. We present insights gained from the synergy between glider observations, time-series measurements and remote sensing in the subtropical North Pacific. We focus on diel variability observed in dissolved oxygen and bio-optics and approaches to autonomously quantify net community production and gross primary production (GPP) as developed during the 2012 Hawaii Ocean Experiment - DYnamics of Light And Nutrients (HOE-DYLAN). Glider-based GPP measurements were extended to explore the relationship between GPP and mesoscale context over multiple years of Seaglider deployments.

Slocum Gliders - Advances in the Technology

NASA Astrophysics Data System (ADS)

Jones, Clayton; Allsup, Ben

2013-04-01

"The projects that I have always liked best are the ones conceived on the spur of the moment by an inquisitive individual. We try to reserve twenty percent of our Slocums to pursue such sudden inspirations. They are generally the most exciting; they evolve in unexpected ways and reveal new dimensions of the unknown about the ocean." Henry Stommel, The SLOCUM Mission, 1989. Abstract Two decades have passed since Stommel's futuristic article popularized Doug Webb's underwater glider concept. Stommel's imagination was sparked by the opportunity gliders provided to broaden our understanding of the oceans and perhaps even more important to him, by the potential it had to draw peoples interest and excitement for ocean dynamics. Slocum Gliders have helped to redefine range and endurance in the world of AUVs, and as mobile sensor platforms have made a significant impact on our ability to better see into the world's oceans. Teledyne Webb Research will provide an overview of the latest Slocum G2 design; the hybrid thruster, increased buoyancy pump displacement, latest sensor suites, and an update on the energy harvesting Slocum Thermal E Twin. Three main mission objectives will be reviewed: polar regions, urban shelves, and long duration transects approximating the course of the Challenger expedition of 1872-76. Having made the first ever oceanic crossing by an AUV, Slocum glider RU27 is presently on display at the Smithsonian Ocean Hall. Another Slocum G2 named Silbo, having completed the Iceland - Azores - Canary Islands legs, is now on its way across the Southern Atlantic to Brazil and continues to draw together the International Consortium of Ocean Observing Labs (I-COOL) focusing on international and educational outreach. With the advent of procurements of Slocum gliders in large numbers by both the US Navy and the Ocean Observation Initiative (OOI) Coastal and Open Ocean we are on the cusp to realizing Stommel's vision of fleets roaming the oceans.

Suicidal hanging within an automobile.

PubMed

Blanco Pampin, J M; López-Abajo Rodriguez, B A

2001-12-01

Accidental asphyxia related to cars has been described in different reports, but suicidal hanging in an automobile is very unusual. Two cases of suicidal hanging inside an automobile are described, illustrating an unusual form of hanging. In one case, the deceased used his belt as a ligature, and the point of attachment was the window of the car. The second victim used the safety belt of the passenger seat. In both cases, the automobile engine was turned off, all the windows were closed, and the door locks were blocked. The medicolegal cause of hanging was based on the scene of the investigation, police and witness reports, social history, autopsy findings, and toxicologic examinations.

Timescales of Equatorward Transport through the Solomon Sea from Glider and Altimetry

NASA Astrophysics Data System (ADS)

Hristova, H. G.; Kessler, W. S.; Davis, R.

2016-12-01

Passage through the semi-enclosed Solomon Sea is the last hurdle in the equatorward journey of the South Pacific western boundary currents before reaching the equator where they contribute to the mass, heat and salt budgets of the equatorial Pacific. We use satellite sea surface height (SSH) and in-situ data from 10 years of glider observations in the Solomon Sea to relate surface geostrophic currents to equatorward transport variability estimated from the gliders. The interior Solomon Sea has enhanced SSH variability compared to the surrounding ocean — its magnitude is largest on ENSO timescales, but also includes significant contributions from the annual and intraseasonal (<120 days) frequencies. Intraseasonal surface variability is dominated by basin-scale, westward propagating disturbances with 60-90 day period, consistent with basin resonance. Because the period of these disturbances is comparable to the time it takes a glider to complete a section across the Sea, the energetic intraseasonal variability is aliased in the glider data and results in section to section spikes in the glider transport estimates. Lower frequency (interannual and annual) SSH correlates well with dynamic height relative to 500m from the glider. Thus, a good lower frequency transport time-series can be obtained from SSH alone. However, the glider provides in addition a vertical structure for the mass transport, as well as estimates of heat and salt transport through the Sea. Two major El Nino events (2009/2010 and 2015/2016) occurred during the glider observation period, both of which show a distinct signature in the mass and heat transport anomalies.

Measurement of the Forces Acting on Gliders in Towed Flight

NASA Technical Reports Server (NTRS)

Klenperer, W B

1940-01-01

The magnitude, the direction, and the fluctuations of tow forces exerted upon gliders by towing them aloft behind an automobile were measured under a variety of conditions covering a range from gentle to severe types of operation. For these tests, the glider towing force did not exceed 1.6 of the gross weight of the glider. V-G records obtained during the towed-flight period as well as during the subsequent return glide to earth showed accelerations in the range from 3 to -1 g. The results of preliminary airplane tow tests are also presented.

A Glider-Assisted Link Disruption Restoration Mechanism in Underwater Acoustic Sensor Networks.

PubMed

Jin, Zhigang; Wang, Ning; Su, Yishan; Yang, Qiuling

2018-02-07

Underwater acoustic sensor networks (UASNs) have become a hot research topic. In UASNs, nodes can be affected by ocean currents and external forces, which could result in sudden link disruption. Therefore, designing a flexible and efficient link disruption restoration mechanism to ensure the network connectivity is a challenge. In the paper, we propose a glider-assisted restoration mechanism which includes link disruption recognition and related link restoring mechanism. In the link disruption recognition mechanism, the cluster heads collect the link disruption information and then schedule gliders acting as relay nodes to restore the disrupted link. Considering the glider's sawtooth motion, we design a relay location optimization algorithm with a consideration of both the glider's trajectory and acoustic channel attenuation model. The utility function is established by minimizing the channel attenuation and the optimal location of glider is solved by a multiplier method. The glider-assisted restoration mechanism can greatly improve the packet delivery rate and reduce the communication energy consumption and it is more general for the restoration of different link disruption scenarios. The simulation results show that glider-assisted restoration mechanism can improve the delivery rate of data packets by 15-33% compared with cooperative opportunistic routing (OVAR), the hop-by-hop vector-based forwarding (HH-VBF) and the vector based forward (VBF) methods, and reduce communication energy consumption by 20-58% for a typical network's setting.

Problem of glider models

NASA Technical Reports Server (NTRS)

Lippisch, Espenlaub

1922-01-01

Any one endeavoring to solve the problem of soaring flight is confronted not only by structural difficulties, but also by the often far more difficult aerodynamic problem of flight properties and efficiency, which can only be determined by experimenting with the finished glider.

Axisymmetric Liquid Hanging Drops

ERIC Educational Resources Information Center

Meister, Erich C.; Latychevskaia, Tatiana Yu

2006-01-01

The geometry of drops hanging on a circular capillary can be determined by numerically solving a dimensionless differential equation that is independent on any material properties, which enables one to follow the change of the height, surface area, and contact angle of drops hanging on a particular capillary. The results show that the application…

Hang-It-Up Artists

ERIC Educational Resources Information Center

Szekely, George

2009-01-01

The best lesson ideas often derive from childhood recollections. When brought to class, play memories and art mementos from the teacher's childhood paint a powerful homage to children's art. A survey of a child's room in the dark, or with lights on, discloses interesting hanging sites, means of attachment and unusual items drafted for hanging, all…

[Sensory illusions in hang-gliding].

PubMed

Bousquet, F; Bizeau, A; Resche-Rigon, P; Taillemite, J P; De Rotalier

1997-01-01

Sensory illusions in hang-gliding and para-gliding. Hang-gliding and para-gliding are at the moment booming sports. Sensory illusions are physiological phenomena sharing the wrong perception of the pilote's real position in space. These phenomena are very familiar to aeroplane pilotes, they can also be noticed on certain conditions with hang-gliding pilotes. There are many and various sensory illusions, but only illusions of vestibular origin will be dealt with in this article. Vestibular physiology is reminded with the working principle of a semicircular canal. Physiology and laws of physics explain several sensory illusions, especially when the pilote loses his visual landmarks: flying through a cloud, coriolis effect. Also some specific stages of hang-gliding foster those phenomena: spiraling downwards, self-rotation, following an asymetric closing of the parachute, spin on oneself. Therefore a previous briefing for the pilotes seems necessary.

RuCool Operational Oceanography: Using a Fleet of Autonomous Ocean Gliders

NASA Astrophysics Data System (ADS)

Graver, J.; Jones, C.; Glenn, S.; Kohut, J.; Schofield, O.; Roarty, H.; Aragon, D.; Kerfoot, J.; Haldeman, C.; Yan, A.

2007-05-01

At the Rutgers University Coastal Ocean Observation Lab (RU-COOL), we have constructed a shelf-wide ocean observatory to characterize the physical forcing of continental shelf primary productivity in the New York Bight (NYB). The system is anchored by four enabling technologies, which include the international constellation of ocean color satellites, multi-static high frequency long-range surface current radar, real-time telemetry moorings, and long duration autonomous underwater vehicles (AUVs). Operation of the observatory is through a centralized computer network dedicated to receiving, processing and visualizing the real-time data and then disseminating results to both field scientists and ocean forecasters over the World Wide Web. The system was designed to conduct cutting edge research requiring the addition of rapidly evolving technologies, and to serve society by providing sustained data delivered in real-time. Rutgers COOL continues to work closely with Webb Research Corporation (WRC) in testing and development of the Slocum underwater gliders and continues to apply Slocum gliders in field operations spanning the globe. The continued strong collaboration between WRC and Rutgers has led to advances in glider operations and applications. These include deployment/recovery techniques, improvements in durability and reliability, integrated sensors suites, salinity spike removal, and adaptive controls utilized to optimize mission goals and data return. The gliders have gathered numerous data sets including salt intrusions as seen off of New Jersey, plume tracking, biological water sample matching, and operation through Hurricane Ernesto in 2006. This talk will detail recent oceanographic experiments in which the fleet has been deployed and improvements in the operation of these novel robotic vehicles. These experiments, in locations around the world, have resulted in significant new work in operation of underwater gliders and have gathered new and unique data

77 FR 71359 - Airworthiness Directives; DG Flugzeugbau GmbH Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-30

...H Gliders AGENCY: Federal Aviation Administration (FAA), Department of Transportation (DOT). ACTION... DG Flugzeugbau GmbH Model DG-1000T gliders equipped with Solo Kleinmotoren Model 2350 C engines. This... proposed AD, contact Solo Kleinmotoren GmbH, Postfach 60 01 52, D 71050 Sindelfingen, Germany; telephone...

Feeding behavior and nutrition of the sugar glider (Petaurus breviceps).

PubMed

Dierenfeld, Ellen S

2009-05-01

Despite the sugar glider's popularity as a pet and a long-term history of captive management in zoologic institutions, little is known concerning their specific nutritional requirements, apart from low basal energy and protein needs. Sugar gliders feed on plant and insect exudates-saps, gums, nectar, manna, honeydew, and lerp-as energy sources and rely on pollen and arthropods for dietary protein. Captive diets based on nutritionally balanced, commercially available products developed for other species, with added produce, have been fed successfully in zoo and private glider colonies, but these diets may not promote optimal gut function or feeding behaviors. Diets commonly fed by private owners were examined in feeding trials and were found to be highly digestible, but contained excess protein that was likely imbalanced in amino acids, as well as in calcium and phosphorus, because of improper supplementation. Suggestions are outlined for areas of relevant research to improve nutritional husbandry of sugar gliders.

Lateral Coherence and Mixing in the Coastal Ocean: Adaptive Sampling using Gliders

DTIC Science & Technology

2012-09-30

Adaptive Sampling using Gliders R. Kipp Shearman Jonathan D. Nash James N. Moum John A. Barth College of Oceanic & Atmospheric Sciences Oregon State...persistent on O (3 day) timescales, so are ideally suited to be adaptively sampled by autonomous gliders that actively report both turbulent and...plan to deploy 4 AUV gliders to perform intensive, adaptive surveys. Newly-enhanced to measure turbulent mixing, water-column currents and dye

Lateral Coherence and Mixing in the Coastal Ocean: Adaptive Sampling using Gliders

DTIC Science & Technology

2011-09-30

Coherence and Mixing in the Coastal Ocean: Adaptive Sampling using Gliders R. Kipp Shearman Jonathan D. Nash James N. Moum John A. Barth College of...These structures evolve yet are often persistent on O (3 day) timescales, so are ideally suited to be adaptively sampled by autonomous gliders that...processes driving lateral dispersion, we plan to deploy 4 AUV gliders to perform intensive, adaptive surveys. Newly-enhanced to measure turbulent mixing

78 FR 67013 - Airworthiness Directives; DG Flugzeugbau GmbH Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-08

... Airworthiness Directives; DG Flugzeugbau GmbH Gliders AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... Flugzeugbau GmbH Models DG-800A, DG-800B, DG-500MB gliders. This AD results from mandatory continuing... holidays. For service information identified in this AD, contact DG- Flugzeugbau GmbH, 76646 Bruchsal...

78 FR 9792 - Airworthiness Directives; DG Flugzeugbau GmbH Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-12

... Airworthiness Directives; DG Flugzeugbau GmbH Gliders AGENCY: Federal Aviation Administration (FAA), Department... (AD) for DG Flugzeugbau GmbH Model DG-1000T gliders equipped with Solo Kleinmotoren Model 2350 C..., contact Solo Kleinmotoren GmbH, Postfach 60 01 52, D 71050 Sindelfingen, Germany; telephone: +49 07031-301...

Agonal sequences in four filmed hangings: analysis of respiratory and movement responses to asphyxia by hanging.

PubMed

Sauvageau, Anny

2009-01-01

The human pathophysiology of asphyxia by hanging is still poorly understood, despite great advances in forensic science. In that context, filmed hangings may hold the key to answer questions regarding the sequence of events leading to death in human asphyxia. Four filmed hangings were analyzed. Rapid loss of consciousness was observed between 13 sec and 18 sec after onset of hanging, closely followed by convulsions (at 14-19 sec). A complex pattern of decerebration rigidity (19-21 sec in most cases), followed by a quick phase of decortication rigidity (1 min 00 sec-1 min 08 sec in most cases), an extended phase of decortication rigidity (1 min 04 sec-1 min 32 sec) and loss of muscle tone (1 min 38 sec-2 min 47 sec) was revealed. Very deep respiratory attempts started between 20 and 22 sec, the last respiratory attempt being detected between 2 min 00 sec and 2 min 04 sec. Despite differences in the types of hanging, this unique study reveals similarities that are further discussed.

A glider swing intervention for people with dementia.

PubMed

Snyder, M; Tseng, Y; Brandt, C; Croghan, C; Hanson, S; Constantine, R; Kirby, L

2001-01-01

The soothing, repetitive movement of rocking and swinging is well known to anyone who has enjoyed a porch swing or rocking chair. Positive effects of rocking have been reported. However, investigations on its effect in people with dementia are limited. The purposes of this quasi-experimental, repeated-measures design study were to measure the effects of a glider swing on emotions, relaxation, and aggressive behaviors in a group of nursing home residents with dementia (n = 30). Data were obtained during a 5-day baseline phase, a 10-day intervention phase, and a 5-day posttreatment phase. Subjects were placed on the glider for 20 minutes each day during the intervention phase. The results of the study indicate that the glider intervention significantly improved emotions and relaxation. The most noted changes were found after 10 minutes of swinging. However, no differences were found in aggressive behaviors.

Cross-coherent vector sensor processing for spatially distributed glider networks.

PubMed

Nichols, Brendan; Sabra, Karim G

2015-09-01

Autonomous underwater gliders fitted with vector sensors can be used as a spatially distributed sensor array to passively locate underwater sources. However, to date, the positional accuracy required for robust array processing (especially coherent processing) is not achievable using dead-reckoning while the gliders remain submerged. To obtain such accuracy, the gliders can be temporarily surfaced to allow for global positioning system contact, but the acoustically active sea surface introduces locally additional sensor noise. This letter demonstrates that cross-coherent array processing, which inherently mitigates the effects of local noise, outperforms traditional incoherent processing source localization methods for this spatially distributed vector sensor network.

Case reports of insulin-dependent glider pilots in the United Kingdom.

PubMed

Saundby, R P

1998-10-01

Insulin-dependent diabetics have not been licensed to fly as pilots. In the United Kingdom, the standards for flying gliders solo were adopted from those required for driving licences, allowing diabetics to fly gliders. No accidents had been reported. My hypothesis was that insulin-dependent diabetics can fly as pilots without danger. A survey was conducted among insulin-dependent glider pilots, using a questionnaire to determine their flying experience and establish any difficulties arising from their disease. Respondents reported that their blood sugar level could be managed in flight and that while complications of diabetes occurred, they had not presented a flight safety hazard. Insulin dependent diabetic pilots flying solo recreational aircraft are not at greater risk than when driving road vehicles. The decision by the British Gliding Association to allow insulin dependent pilots to fly gliders solo has been justified by experience.

14 CFR 61.69 - Glider and unpowered ultralight vehicle towing: Experience and training requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Glider and unpowered ultralight vehicle... GROUND INSTRUCTORS Aircraft Ratings and Pilot Authorizations § 61.69 Glider and unpowered ultralight... a glider or unpowered ultralight vehicle unless that person— (1) Holds a private, commercial or...

14 CFR 61.69 - Glider and unpowered ultralight vehicle towing: Experience and training requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Glider and unpowered ultralight vehicle... GROUND INSTRUCTORS Aircraft Ratings and Pilot Authorizations § 61.69 Glider and unpowered ultralight... a glider or unpowered ultralight vehicle unless that person— (1) Holds a private, commercial or...

14 CFR 61.69 - Glider and unpowered ultralight vehicle towing: Experience and training requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Glider and unpowered ultralight vehicle... GROUND INSTRUCTORS Aircraft Ratings and Pilot Authorizations § 61.69 Glider and unpowered ultralight... a glider or unpowered ultralight vehicle unless that person— (1) Holds a private, commercial or...

14 CFR 61.69 - Glider and unpowered ultralight vehicle towing: Experience and training requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Glider and unpowered ultralight vehicle... GROUND INSTRUCTORS Aircraft Ratings and Pilot Authorizations § 61.69 Glider and unpowered ultralight... a glider or unpowered ultralight vehicle unless that person— (1) Holds a private, commercial or...

14 CFR 61.69 - Glider and unpowered ultralight vehicle towing: Experience and training requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Glider and unpowered ultralight vehicle... GROUND INSTRUCTORS Aircraft Ratings and Pilot Authorizations § 61.69 Glider and unpowered ultralight... a glider or unpowered ultralight vehicle unless that person— (1) Holds a private, commercial or...

The glider balloon: a useful device for the treatment of bifurcation lesions.

PubMed

Briguori, Carlo; Visconti, Gabriella; Donahue, Michael; Chiariello, Giovanni Alfonso; Focaccio, Amelia

2013-10-09

Final kissing balloon dilatation (FKBD) is a recommended final step in case of treatment of bifurcation lesions by two stents approaches. Furthermore, dilatation of the side branch (SB) may be necessary following main vessel (MV) stenting. Occasionally, recrossing the stent struts with a balloon is hampered because the tip hits a stent strut. The Glider (TriReme Medical, Pleasanton, CA) is a dedicated balloon designed for crossing through struts of deployed stents toward a SB. From October 2010 to January 2012, FKBD was attempted in 236 consecutive bifurcation lesions treated in our Institution. FKBD was successfully performed by conventional balloon catheters in 221 (93.5%) lesions (Conventional group). In the remaining 15 (6.5%) lesions, where a conventional balloon failed to cross the stent strut, the Glider balloon was attempted (Glider group). The angle beta (between the axis of the MV after the branch point and the SB axis at the point of divergence) was wider in the Glider group (83±17° versus 65±27°; p=0.032). A trend toward an higher rate of the true bifurcation lesions was observed in the Glider group (93% versus 70.5%; p=0.07). The Glider balloon successfully crossed through MV stent struts toward a SB in 12 patients (80%), whereas failed in the remaining 3 patients. The Glider balloon represents an unique bail-out device which offers an effective rescue strategy for recrossing stent struts during complex bifurcation stenting. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

78 FR 65869 - Airworthiness Directives; DG Flugzeugbau GmbH Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-04

... Directives; DG Flugzeugbau GmbH Gliders AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final... Flugzeugbau GmbH Model DG-1000T glider equipped with a Solo Kleinmotoren Model 2350 C engine. This AD results... in this AD, contact Solo Kleinmotoren GmbH, Postfach 60 01 52, D 71050 Sindelfingen, Germany...

Parametric geometric model and shape optimization of an underwater glider with blended-wing-body

NASA Astrophysics Data System (ADS)

Sun, Chunya; Song, Baowei; Wang, Peng

2015-11-01

Underwater glider, as a new kind of autonomous underwater vehicles, has many merits such as long-range, extended-duration and low costs. The shape of underwater glider is an important factor in determining the hydrodynamic efficiency. In this paper, a high lift to drag ratio configuration, the Blended-Wing-Body (BWB), is used to design a small civilian under water glider. In the parametric geometric model of the BWB underwater glider, the planform is defined with Bezier curve and linear line, and the section is defined with symmetrical airfoil NACA 0012. Computational investigations are carried out to study the hydrodynamic performance of the glider using the commercial Computational Fluid Dynamics (CFD) code Fluent. The Kriging-based genetic algorithm, called Efficient Global Optimization (EGO), is applied to hydrodynamic design optimization. The result demonstrates that the BWB underwater glider has excellent hydrodynamic performance, and the lift to drag ratio of initial design is increased by 7% in the EGO process.

Glider kits cut truck costs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1984-02-01

Trucks used for the delivery of coal have a relatively limited life because they must be specified for somewhat less than brute strength in order to achieve maximum payload within existing weight and length limitations. The major drive train components such as engine, transmission, and drive axles can last longer if rebuilt or remanufactured periodically. For that reason glider kits and other truck components ready for the installation of the drive chain are becoming increasingly popular. These kits have been available for many years, but were regarded only as a means of salvaging late model wrecks or burned trucks. Theirmore » recent acceptance as a means of periodic fleet updating is built around the low cost and immediate availability of remanufactured major components. The cost advantages in using these glider kits are discussed.« less

76 FR 253 - Airworthiness Directives; ROLLADEN-SCHNEIDER Flugzeugbau GmbH Model LS6 Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-04

... Airworthiness Directives; ROLLADEN-SCHNEIDER Flugzeugbau GmbH Model LS6 Gliders AGENCY: Federal Aviation... condition does not exist in the Model LS6-c gliders. FAA's Determination We are issuing this AD rescission... Model LS6 glider, and the unsafe condition described previously is not likely to exist or develop in the...

Flight Software Development for the Liberdade Flying Wing Glider

DTIC Science & Technology

2013-12-24

gliders. Bigger gliders are more efficient at horizontal transport. Surveys of natural and man-made flyers ( McMasters , 1974) confirm this relation...The other benefit of a large wing area is that it reduces the coefficient of lift and the associated induced drag (the largest component of drag at...greater reduction in specific energy consumption than does a proportionally smaller lift coefficient . Increases in aspect ratio, in turn, must be

EGO: Towards a global glider infrastructure for the benefit of marine research and operational oceanography

NASA Astrophysics Data System (ADS)

Testor, Pierre

2013-04-01

In the 1990 s, while gliders were being developed and successfully passing first tests, their potential use for ocean research started to be discussed in international conferences because they could help us improve the cost-effectiveness, sampling, and distribution of the ocean observations (see OceanObs'99 Conference Statement - UNESCO). After the prototype phase, in the 2000 s, one could only witness the growing glider activity throughout the world. The first glider experiments in Europe brought together several teams that were interested in the technology and a consortium formed naturally from these informal collaborations. Since 2006, Everyone's Gliding Observatories (EGO - http://www.ego-network.org) Workshops and Glider Schools have been organized, whilst becoming the international forum for glider activities. Some key challenges have emerged from the expansion of the glider system and require now setting up a sustainable European as well as a global system to operate glider and to ensure a smooth and sustained link to the Global Ocean Observing System (GOOS). Glider technology faces many scientific, technological and logistical issues. In particular, it approaches the challenge of controlling many steerable probes in a variable environment for better sampling. It also needs the development of new formats and procedures in order to build glider observatories at a global level. Several geographically distributed teams of oceanographers now operate gliders, and there is a risk of fragmentation. We will here present results from our consortium who intends to solve most of these issues through scientific and technological coordination and networking. This approach is supported by the ESF through Cooperation in the field of Scientific and Technical Research (COST). The COST Action ES0904 "EGO" started in July 2010 aiming to build international cooperation and capacities at the scientific, technological, and organizational levels, for sustained observations of the

Depth-averaged instantaneous currents in a tidally dominated shelf sea from glider observations

NASA Astrophysics Data System (ADS)

Merckelbach, Lucas

2016-12-01

Ocean gliders have become ubiquitous observation platforms in the ocean in recent years. They are also increasingly used in coastal environments. The coastal observatory system COSYNA has pioneered the use of gliders in the North Sea, a shallow tidally energetic shelf sea. For operational reasons, the gliders operated in the North Sea are programmed to resurface every 3-5 h. The glider's dead-reckoning algorithm yields depth-averaged currents, averaged in time over each subsurface interval. Under operational conditions these averaged currents are a poor approximation of the instantaneous tidal current. In this work an algorithm is developed that estimates the instantaneous current (tidal and residual) from glider observations only. The algorithm uses a first-order Butterworth low pass filter to estimate the residual current component, and a Kalman filter based on the linear shallow water equations for the tidal component. A comparison of data from a glider experiment with current data from an acoustic Doppler current profilers deployed nearby shows that the standard deviations for the east and north current components are better than 7 cm s-1 in near-real-time mode and improve to better than 6 cm s-1 in delayed mode, where the filters can be run forward and backward. In the near-real-time mode the algorithm provides estimates of the currents that the glider is expected to encounter during its next few dives. Combined with a behavioural and dynamic model of the glider, this yields predicted trajectories, the information of which is incorporated in warning messages issued to ships by the (German) authorities. In delayed mode the algorithm produces useful estimates of the depth-averaged currents, which can be used in (process-based) analyses in case no other source of measured current information is available.

Natural hydrocarbon seeps observation with underwater gliders and UV fluorescence sensor

NASA Astrophysics Data System (ADS)

Rochet, V.

2016-02-01

Hydrocarbons may leak to the near-surface from subsurface accumulations, from mature source rock, or by buoyancy along major cross-strata routes. The presence of migrating near-surface hydrocarbons can provide strong evidence for the presence of a working petroleum system, as well as valuable information on source, maturity, and migration pathways. Detection and characterization of hydrocarbons in the water column may then help to de-risk hydrocarbon plays at a very preliminary stage of an exploration program. In order to detect hydrocarbons in the water column, an underwater glider survey was conducted in an offshore frontier area. Driven by buoyancy variation, underwater gliders enable collecting data autonomously along the water column for weeks to months. Underwater gliders are regularly piloted from shore by satellite telemetry and do not require a surface supervising vessel resulting in substantial operational costs savings. The data compiled, over 700m depth of the water column, included temperature, salinity, pressure, dissolved oxygen and hydrocarbon components (phenanthrene and naphthalene) measured by "MINIFLUO" sensors to particularly target representative crude oil compounds Two gliders were deployed at sea, one from coast in shallow water and the other one offshore on the survey area. Both accurately squared the survey area following pre-defined lines and cross lines. Data files were transmitted by satellite telemetry in near real time during the performance of the mission for real time observations and appropriate re-positioning of the gliders. Using rechargeable underwater gliders increased reliability reducing the risk of leakage and associated logistics during operation at sea. Despite strong evidences of seabed seepages such as pockmarks, faults, etc, over the area of interest, no hydrocarbon indices were detected in the water column, which was confirmed later by seabed sample analysis. The use of glider platforms for hydrocarbon detection has

Verifying the Hanging Chain Model

ERIC Educational Resources Information Center

Karls, Michael A.

2013-01-01

The wave equation with variable tension is a classic partial differential equation that can be used to describe the horizontal displacements of a vertical hanging chain with one end fixed and the other end free to move. Using a web camera and TRACKER software to record displacement data from a vibrating hanging chain, we verify a modified version…

Agonal sequences in a filmed suicidal hanging: analysis of respiratory and movement responses to asphyxia by hanging.

PubMed

Sauvageau, Anny; Racette, Stéphanie

2007-07-01

The forensic literature on the pathophysiology of human hanging is still limited. Therefore, forensic pathologists often feel uncomfortable when confronted with related questions. Here presented is the filmed suicidal hanging of a 37-year-old man. This recording allows a unique analysis of agonal movement sequences: loss of consciousness (13 sec), convulsions (15 sec), decortication rigidity (21 sec), decerebration rigidity (46 sec), second decortication rigidity (1 min 11 sec), loss of muscle tone, (1 min 38 sec) and last isolated muscle movement (4 min 10 sec). As for respiratory responses, very deep respiratory attempts started at 20 sec. Respiratory movements progressively decreased and completely stopped at 2 min. Despite the fact that extending the presented data on all cases of hanging asphyxia would be a mistake, this case gives a very interesting insight into movement and respiratory response to asphyxia by hanging.

Expedition 54 plaque hanging ceremony

NASA Image and Video Library

2018-04-26

NASA astronauts Joe Acaba and Mark Vande Hei Acaba visited Marshall April 11 for their honorary Expedition 54 plaque hanging ceremony and to provide valuable feedback of their on-orbit science investigations with the Payload Operations and Integration Center team. Chris Buckley is awarded privilege of hanging Mission 54 placque, shown here with Astronaut Mark Vande Hei (L) and Astronaut Joe Acaba

Preliminary study of tug-glider freight systems utilizing a Boeing 747 as the tug

NASA Technical Reports Server (NTRS)

Heyson, H. H.; Foss, W. E., Jr.

1977-01-01

Performance of the tug-glider system was severely limited by ground run. In most cases studied, additional engines were necessary. Except at short ranges for which additional payload were carried in the tow plane, the productivity of the basic aircraft was degraded by a reduction in cruise speed necessitated by the glider drag. Excessive aspect ratios did not improve system performance because of the increase in glider wing weight. Powered gliders using a tow plane only for takeoff and climb had the potential for a major reduction in fuel consumption. Uncertainty of restrictive regulatory action and the apparently increased airborne investment per unit productivity are obstacles to commercial development.

Retrospective Analysis of Hanging Deaths in Ontario.

PubMed

Tugaleva, Elena; Gorassini, Donald R; Shkrum, Michael J

2016-11-01

Hanging deaths from investigation standpoint are rarely problematic. Unusual circumstances can on occasion raise suspicion of foul play. Associated neck injuries are reported in the literature with variable frequency (from 0% to 76.8%). This study retrospectively analyzed 755 hanging deaths in Ontario (Canada) to evaluate the demographic features and circumstances of hanging fatalities, and the frequency of hanging-related neck injuries. A number of cases showed unusual/special circumstances (e.g., complex, double suicides, restraints). Among 632 cases with complete autopsies, hyoid and larynx fractures were present in 46 cases (7.3%) with the most common being isolated hyoid fractures. The incidence of cricoid fractures was 0.5% and cervical spine injuries, 1.1%. A higher incidence of neck injuries occurred among males, long drop hangings, and in cases with complete suspension. There was a tendency for the number of fractures to increase with increasing age and weight of the deceased. © 2016 American Academy of Forensic Sciences.

Suicidal Decapitation by Hanging-A Population-based Study.

PubMed

Byard, Roger W; Gilbert, John D

2018-05-01

A prospective study was undertaken at Forensic Science SA over a 15-year period from July 2002 to June 2017 for all cases of adult (>18 years) suicidal hangings with decapitation. A total of 1446 cases of suicidal hangings were identified from a general population of approximately 1.5 million (1206 males-age range 18-97 years, average 42.6; and 240 females-age range 18-96 years, average 40.1). Only three cases of decapitation were found, all from long-drop hangings; these consisted of three males (ages 32-55 years; average 45 years). Spinal transections had occurred between the first and second, second and third, and third and fourth cervical vertebrae, respectively. In this study, the number of suicidal hangings with decapitation represented only 0.2% of the total number of hangings. These events are therefore extremely rare, most likely due to most suicidal hangings occurring from relatively low levels in a domestic environment. © 2017 American Academy of Forensic Sciences.

Analysis of the Flight Motions of a Small Deployable Glider Configuration

NASA Technical Reports Server (NTRS)

Coe, Paul L., Jr.

1975-01-01

An investigation was conducted at the request of the U.S. Air Force Avionics Laboratory to analyze the flight characteristics of a small uncontrolled glider with folding wings. The study consisted of wind-tunnel tests of an actual glider and a theoretical analysis of the performance, stability, and trimmability of the configuration.

NRL Glider Data Report for the Shelf-Slope Experiment

DTIC Science & Technology

2017-09-12

until 16 February, 2016. The glider made a total of 349 survey profiles within the geographical box bounded between 28.95N to 29.25N and 88.6W to 88.25W...12-09-2017 Memorandum Report Gulf of Mexico Oceanography Ocean optics Glider survey 73-1D52-05-5 The University of Southern Mississippi 118 College... survey corresponds to when Salinity increased, probably in water less affected by Mississippi outflow. 14 Figure 13. Chlorophyll waterfall image plot

Real-time reporting of baleen whale passive acoustic detections from ocean gliders.

PubMed

Baumgartner, Mark F; Fratantoni, David M; Hurst, Thomas P; Brown, Moira W; Cole, Tim V N; Van Parijs, Sofie M; Johnson, Mark

2013-09-01

In the past decade, much progress has been made in real-time passive acoustic monitoring of marine mammal occurrence and distribution from autonomous platforms (e.g., gliders, floats, buoys), but current systems focus primarily on a single call type produced by a single species, often from a single location. A hardware and software system was developed to detect, classify, and report 14 call types produced by 4 species of baleen whales in real time from ocean gliders. During a 3-week deployment in the central Gulf of Maine in late November and early December 2012, two gliders reported over 25,000 acoustic detections attributed to fin, humpback, sei, and right whales. The overall false detection rate for individual calls was 14%, and for right, humpback, and fin whales, false predictions of occurrence during 15-min reporting periods were 5% or less. Transmitted pitch tracks--compact representations of sounds--allowed unambiguous identification of both humpback and fin whale song. Of the ten cases when whales were sighted during aerial or shipboard surveys and a glider was within 20 km of the sighting location, nine were accompanied by real-time acoustic detections of the same species by the glider within ±12 h of the sighting time.

75 FR 74608 - Airworthiness Directives; CENTRAIR Models 101, 101A, 101P, and 101AP Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-01

... Airworthiness Directives; CENTRAIR Models 101, 101A, 101P, and 101AP Gliders AGENCY: Federal Aviation...[eacute] Nouvelle (SN) Centrair. This tube had been reinforced in 1984 with a modification. Gliders...[eacute] Nouvelle (SN) Centrair. This tube had been reinforced in 1984 with a modification. Gliders...

Preliminary study of tug-glider freight systems utilizing a Boeing 747 as the tug

DOE Office of Scientific and Technical Information (OSTI.GOV)

Heyson, H.H.; Foss, W.E. Jr

1977-02-01

Performance of the tug-glider system was severely limited by ground run. In most cases studied, additional engines were necessary. Except at short ranges for which additional payload were carried in the tow plane, the productivity of the basic aircraft was degraded by a reduction in cruise speed necessitated by the glider drag. Excessive aspect ratios did not improve system performance because of the increase in glider wing weight. Powered gliders using a tow plane only for takeoff and climb had the potential for a major reduction in fuel consumption. Uncertainty of restrictive regulatory action and the apparently increased airborne investmentmore » per unit productivity are obstacles to commercial development.« less

Flight Test of GL-1 Glider Half Scale Prototype

NASA Astrophysics Data System (ADS)

Fikri Zulkarnain, Muhammad; Fazlur Rahman, Muhammad; Luthfi Imam Nurhakim, Muhammad; Arifianto, Ony; Mulyanto, Taufiq

2018-04-01

GL-1 is a single-seat mid-performance glider, designed to be Indonesian National Glider. The Glider have been developing since 2014. The development produced a half scale prototype called BL-1, which had accomplished static test in 2016, then followed by first flight test at April 20th 2017, and second flight test at May 21st 2017. The purpose of the flight test was to obtain familiarization of the aircraft, aerodynamics characteristics and flow visualization, with data from flight recorded in FDR. The flight test resulted in two flights with total length of 21 minutes. The data from FDR and flight test documents extracted to analyze the characteristics and behavior of the aircraft during flight test. The aerodynamics characteristic was close to analytical results. The control was good; however, the effectiveness of control surface may need to be further analyzed. The result of the flight test will be used as a reference for further improvements and may need further testing.

76 FR 9495 - Feathering Propeller Systems for Light-Sport Aircraft Powered Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-18

.... No. 1-66] RIN 2120-AJ81 Feathering Propeller Systems for Light-Sport Aircraft Powered Gliders AGENCY... rule amends the definition of light-sport aircraft by removing ``auto'' from the term ``autofeathering... operation for powered gliders that qualify as light-sport aircraft. DATES: The effective date for the final...

Near-Slope changes in the Eurasian and Makarov Basins from Glider Surveys

DTIC Science & Technology

2015-09-30

vibration and vehicle orientation. The Microrider samples all channels at a constant 512 Hz rate, and is neutrally buoyant in seawater , which allows for...efficient and precise ballasting of the glider vehicle. The lithium- battery powered G2 gliders will also be equipped with a pumped Seabird GPCTD, a

Mapping sub-surface geostrophic currents from altimetry and a fleet of gliders

NASA Astrophysics Data System (ADS)

Alvarez, A.; Chiggiato, J.; Schroeder, K.

2013-04-01

Integrating the observations gathered by different platforms into a unique physical picture of the environment is a fundamental aspect of networked ocean observing systems. These are constituted by a spatially distributed set of sensors and platforms that simultaneously monitor a given ocean region. Remote sensing from satellites is an integral part of present ocean observing systems. Due to their autonomy, mobility and controllability, underwater gliders are envisioned to play a significant role in the development of networked ocean observatories. Exploiting synergism between remote sensing and underwater gliders is expected to result on a better characterization of the marine environment than using these observational sources individually. This study investigates a methodology to estimate the three dimensional distribution of geostrophic currents resulting from merging satellite altimetry and in situ samples gathered by a fleet of Slocum gliders. Specifically, the approach computes the volumetric or three dimensional distribution of absolute dynamic height (ADH) that minimizes the total energy of the system while being close to in situ observations and matching the absolute dynamic topography (ADT) observed from satellite at the sea surface. A three dimensional finite element technique is employed to solve the minimization problem. The methodology is validated making use of the dataset collected during the field experiment called Rapid Environmental Picture-2010 (REP-10) carried out by the NATO Undersea Research Center-NURC during August 2010. A marine region off-shore La Spezia (northwest coast of Italy) was sampled by a fleet of three coastal Slocum gliders. Results indicate that the geostrophic current field estimated from gliders and altimetry significantly improves the estimates obtained using only the data gathered by the glider fleet.

Shelf sea tidal currents and mixing fronts determined from ocean glider observations

NASA Astrophysics Data System (ADS)

Sheehan, Peter M. F.; Berx, Barbara; Gallego, Alejandro; Hall, Rob A.; Heywood, Karen J.; Hughes, Sarah L.; Queste, Bastien Y.

2018-03-01

Tides and tidal mixing fronts are of fundamental importance to understanding shelf sea dynamics and ecosystems. Ocean gliders enable the observation of fronts and tide-dominated flows at high resolution. We use dive-average currents from a 2-month (12 October-2 December 2013) glider deployment along a zonal hydrographic section in the north-western North Sea to accurately determine M2 and S2 tidal velocities. The results of the glider-based method agree well with tidal velocities measured by current meters and with velocities extracted from the TPXO tide model. The method enhances the utility of gliders as an ocean-observing platform, particularly in regions where tide models are known to be limited. We then use the glider-derived tidal velocities to investigate tidal controls on the location of a front repeatedly observed by the glider. The front moves offshore at a rate of 0.51 km day-1. During the first part of the deployment (from mid-October until mid-November), results of a one-dimensional model suggest that the balance between surface heat fluxes and tidal stirring is the primary control on frontal location: as heat is lost to the atmosphere, full-depth mixing is able to occur in progressively deeper water. In the latter half of the deployment (mid-November to early December), a front controlled solely by heat fluxes and tidal stirring is not predicted to exist, yet a front persists in the observations. We analyse hydrographic observations collected by the glider to attribute the persistence of the front to the boundary between different water masses, in particular to the presence of cold, saline, Atlantic-origin water in the deeper portion of the section. We combine these results to propose that the front is a hybrid front: one controlled in summer by the local balance between heat fluxes and mixing and which in winter exists as the boundary between water masses advected to the north-western North Sea from diverse source regions. The glider observations

Impact of glider data assimilation on the Monterey Bay model

NASA Astrophysics Data System (ADS)

Shulman, Igor; Rowley, Clark; Anderson, Stephanie; DeRada, Sergio; Kindle, John; Martin, Paul; Doyle, James; Cummings, James; Ramp, Steve; Chavez, Francisco; Fratantoni, David; Davis, Russ

2009-02-01

Glider observations were essential components of the observational program in the Autonomous Ocean Sampling Network (AOSN-II) experiment in the Monterey Bay area during summer of 2003. This paper is focused on the impact of the assimilation of glider temperature and salinity observations on the Navy Coastal Ocean Model (NCOM) predictions of surface and subsurface properties. The modeling system consists of an implementation of the NCOM model using a curvilinear, orthogonal grid with 1-4 km resolution, with finest resolution around the bay. The model receives open boundary conditions from a regional (9 km resolution) NCOM implementation for the California Current System, and surface fluxes from the Coupled Ocean-Atmosphere Mesoscale Prediction System (COAMPS) atmospheric model at 3 km resolution. The data assimilation component of the system is a version of the Navy Coupled Ocean Data Assimilation (NCODA) system, which is used for assimilation of the glider data into the NCOM model of the Monterey Bay area. The NCODA is a fully 3D multivariate optimum interpolation system that produces simultaneous analyses of temperature, salinity, geopotential, and vector velocity. Assimilation of glider data improves the surface temperature at the mooring locations for the NCOM model hindcast and nowcasts, and for the short-range (1-1.5 days) forecasts. It is shown that it is critical to have accurate atmospheric forcing for more extended forecasts. Assimilation of glider data provided better agreement with independent observations (for example, with aircraft measured SSTs) of the model-predicted and observed spatial distributions of surface temperature and salinity. Mooring observations of subsurface temperature and salinity show sharp changes in the thermocline and halocline depths during transitions from upwelling to relaxation and vice versa. The non-assimilative run also shows these transitions in subsurface temperature; but they are not as well defined. For salinity, the

Exploring Titan with Autonomous, Buoyancy Driven Gliders

NASA Astrophysics Data System (ADS)

Morrow, M. T.; Woolsey, C. A.; Hagerman, G. M.

Buoyancy driven underwater gliders are highly efficient winged underwater vehicles which locomote by modifying their internal shape. The concept, which is already well-proven in Earth's oceans, is also an appealing technology for remote terrain exploration and environmental sampling on worlds with dense atmospheres. Because of their high efficiency and their gentle, vertical take-off and landing capability, buoyancy driven gliders might perform long duration, global mapping tasks as well as light-duty, local sampling tasks. Moreover, a sufficiently strong gradient in the planetary boundary layer may enable the vehicles to perform dynamic soaring, achieving even greater locomotive efficiency. Shape Change Actuated, Low Altitude Robotic Soarers (SCALARS) are an appealing alternative to more conventional vehicle technology for exploring planets with dense atmospheres. SCALARS are buoyancy driven atmospheric gliders with a twin-hulled, inboard wing configuration. The inboard wing generates lift, which propels the vehicle forward. Symmetric changes in mass distribution induce gravitational pitch moments that provide longitudinal control. Asymmetric changes in mass distribution induce twist in the inboard wing that provides directional control. The vehicle is actuated solely by internal shape change; there are no external seals and no exposed moving parts, save for the inflatable buoyancy ballonets. Preliminary sizing analysis and dynamic modeling indicate the viability of using SCALARS to map the surface of Titan and to investigate features of interest.

Outcomes of therapeutic hypothermia in unconscious patients after near-hanging.

PubMed

Lee, Byung Kook; Jeung, Kyung Woon; Lee, Hyoung Youn; Lim, Jae Hoon

2012-09-01

Hanging has been increasingly used to commit suicide. There is no specific treatment besides general intensive care after near-hanging. Therapeutic hypothermia (TH) has been used in unconscious patients after near-hanging. To describe the outcomes in unconscious patients after near-hanging in order to determine whether TH improves the outcome of near-hanging injury. Medical charts were reviewed of unconscious patients after near-hanging who presented to Chonnam National University Hospital between January 2006 and December 2010 and who were considered to be eligible for TH. According to local policy, unconscious survivors after near-hanging, whether or not they experienced cardiac arrest at the scene, were treated with TH if this was agreed by next-of-kin. There were 16 survivors of asphyxial cardiac arrest after near-hanging, of whom 13 received TH. Among them, only one (7.7%, 95% CI 1.4% to 33.3%) attained Cerebral Performance Category (CPC) 1; the other 15 patients had poor neurological outcomes (CPC 5 in seven patients and CPC 4 in eight patients). Nine of the patients did not experience cardiac arrest at the scene and of these, four received TH and five received normothermic treatment. All patients who did not have cardiac arrest recovered and were discharged with CPC 1. In this study, outcomes in unconscious near-hanging patients with cardiac arrest were poor despite treatment with TH. Before recommending TH in near-hanging patients, a prospective, randomised controlled study is required.

Lilienthal's fatal glider crash in 1896: evidence regarding the cause of death.

PubMed

Harsch, Viktor; Bardrum, Benny; Illig, Petra

2008-10-01

Otto Lilienthal's pioneering work on gliders helped form the basis for development of powered aircraft. His death following a glider crash in 1896 was officially ascribed to fracture of the cervical spine. However, the clinical details assembled here make it more likely that he died from head trauma with resulting complications, possibly including intracranial hematoma.

Space Shuttle Glider. Educational Brief.

ERIC Educational Resources Information Center

National Aeronautics and Space Administration, Washington, DC.

Space Shuttle Glider is a scale model of the U.S. Space Shuttle orbiter. The airplane-like orbiter usually remains in Earth orbit for up to two weeks at a time. It normally carries a six- to seven-person crew which includes the mission commander, pilot, and several mission and/or payload specialists who have specialized training associated with…

Estimating uncertainty in subsurface glider position using transmissions from fixed acoustic tomography sources.

PubMed

Van Uffelen, Lora J; Nosal, Eva-Marie; Howe, Bruce M; Carter, Glenn S; Worcester, Peter F; Dzieciuch, Matthew A; Heaney, Kevin D; Campbell, Richard L; Cross, Patrick S

2013-10-01

Four acoustic Seagliders were deployed in the Philippine Sea November 2010 to April 2011 in the vicinity of an acoustic tomography array. The gliders recorded over 2000 broadband transmissions at ranges up to 700 km from moored acoustic sources as they transited between mooring sites. The precision of glider positioning at the time of acoustic reception is important to resolve the fundamental ambiguity between position and sound speed. The Seagliders utilized GPS at the surface and a kinematic model below for positioning. The gliders were typically underwater for about 6.4 h, diving to depths of 1000 m and traveling on average 3.6 km during a dive. Measured acoustic arrival peaks were unambiguously associated with predicted ray arrivals. Statistics of travel-time offsets between received arrivals and acoustic predictions were used to estimate range uncertainty. Range (travel time) uncertainty between the source and the glider position from the kinematic model is estimated to be 639 m (426 ms) rms. Least-squares solutions for glider position estimated from acoustically derived ranges from 5 sources differed by 914 m rms from modeled positions, with estimated uncertainty of 106 m rms in horizontal position. Error analysis included 70 ms rms of uncertainty due to oceanic sound-speed variability.

Listeria monocytogenes Infection in a Sugar Glider (Petaurus breviceps) - New Mexico, 2011.

PubMed

Nichols, M; Takacs, N; Ragsdale, J; Levenson, D; Marquez, C; Roache, K; Tarr, C L

2015-06-01

Listeria monocytogenes is a Gram-positive, facultative anaerobic, rod-shaped bacterium that can infect and cause disease in many species. In this case report, we describe a case of L. monocytogenes infection causing sepsis in a sugar glider (Petaurus breviceps). The sugar glider consumed a varied diet consisting of human food items, including cantaloupe. A nationwide outbreak of L. monocytogenes foodborne illness associated with cantaloupes occurred simultaneously with this incident case. In this case, the bacterial strains from the outbreak and glider were genetically distinct. Although rare, veterinarians should be aware of the emergence of foodborne pathogens' ability to infect exotic animals residing in domestic environments. © 2014 Blackwell Verlag GmbH.

The 2014-2015 Warming Anomaly in the Southern California Current System: Glider Observations

NASA Astrophysics Data System (ADS)

Zaba, K. D.; Rudnick, D. L.

2016-02-01

During 2014-2015, basin-wide patterns of oceanic and atmospheric anomalies affected surface waters throughout the North Pacific Ocean. We present regional physical and biological effects of the warming, as observed by our autonomous underwater gliders in the southern California Current System (SCCS). Established in 2006, the California Glider Network provides sustained subsurface observations for monitoring the coastal effects of large-scale climate variability. Along repeat sections that extend to 350-500 km in offshore distance and 500 m in depth, Spray gliders have continuously occupied CalCOFI lines 66.7, 80, and 90 for nearly nine years. Following a sawtooth trajectory, the gliders complete each dive in approximately 3 hours and over 3 km. Measured variables include pressure, temperature, salinity, chlorophyll fluorescence, and velocity. For each of the three lines, a comprehensive climatology has been constructed from the multiyear timeseries. The ongoing surface-intensified warming anomaly, which began locally in early 2014 and persists through present, is unprecedented in the glider climatology. Reaching up to 5°C, positive temperature anomalies have been generally confined to the upper 50 m and persistent for over 20 months. The timing of the warming was in phase along each glider line but out of phase with equatorial SST anomalies, suggesting a decoupling of tropical and mid-latitude dynamics. Concurrent physical oceanographic anomalies included a depressed thermocline and high stratification. An induced biological response was apparent in the deepening of the subsurface chlorophyll fluorescence maximum. Ancillary atmospheric data from the NCEP North American Mesoscale (NAM) model indicate that a combination of surface forcing anomalies, namely high downward heat flux and weak wind stress magnitude, caused the unusual warm, downwelling conditions. With a strong El Niño event in the forecast for winter 2015-2016, our sustained glider network will

Thermal biology, torpor and behaviour in sugar gliders: a laboratory-field comparison.

PubMed

Geiser, Fritz; Holloway, Joanne C; Körtner, Gerhard

2007-07-01

Most studies on animal physiology and behaviour are conducted in captivity without verification that data are representative of free-ranging animals. We provide the first quantitative comparison of daily torpor, thermal biology and activity patterns, conducted on two groups of sugar gliders (Petaurus breviceps, Marsupialia) exposed to similar thermal conditions, one in captivity and the other in the field. Our study shows that activity in captive gliders in an outdoor aviary is restricted to the night and largely unaffected by weather, whereas free-ranging gliders omit foraging on cold/wet nights and may also forage in the afternoon. Torpor occurrence in gliders was significantly lower in captivity (8.4% after food deprivation; 1.1% for all observations) than in the field (25.9%), mean torpor bout duration was shorter in captivity (6.9 h) than in the field (13.1 h), and mean body temperatures during torpor were higher in captivity (25.3 degrees C) than in the field (19.6 degrees C). Moreover, normothermic body temperature as a function of air temperature differed between captive and free-ranging gliders, with a >3 degrees C difference at low air temperatures. Our comparison shows that activity patterns, thermal physiology, use of torpor and patterns of torpor may differ substantially between the laboratory and field, and provides further evidence that functional and behavioural data on captive individuals may not necessarily be representative of those living in the wild.

A Glider-Assisted Link Disruption Restoration Mechanism in Underwater Acoustic Sensor Networks

PubMed Central

Wang, Ning; Su, Yishan; Yang, Qiuling

2018-01-01

Underwater acoustic sensor networks (UASNs) have become a hot research topic. In UASNs, nodes can be affected by ocean currents and external forces, which could result in sudden link disruption. Therefore, designing a flexible and efficient link disruption restoration mechanism to ensure the network connectivity is a challenge. In the paper, we propose a glider-assisted restoration mechanism which includes link disruption recognition and related link restoring mechanism. In the link disruption recognition mechanism, the cluster heads collect the link disruption information and then schedule gliders acting as relay nodes to restore the disrupted link. Considering the glider’s sawtooth motion, we design a relay location optimization algorithm with a consideration of both the glider’s trajectory and acoustic channel attenuation model. The utility function is established by minimizing the channel attenuation and the optimal location of glider is solved by a multiplier method. The glider-assisted restoration mechanism can greatly improve the packet delivery rate and reduce the communication energy consumption and it is more general for the restoration of different link disruption scenarios. The simulation results show that glider-assisted restoration mechanism can improve the delivery rate of data packets by 15–33% compared with cooperative opportunistic routing (OVAR), the hop-by-hop vector-based forwarding (HH-VBF) and the vector based forward (VBF) methods, and reduce communication energy consumption by 20–58% for a typical network’s setting. PMID:29414898

Radiation profiles measured through clouds using a return glider radiosonde

NASA Astrophysics Data System (ADS)

Kräuchi, Andreas; Philipona, Rolf; Kivi, Rigel

2016-04-01

With new and improved radiation sensors in a small glider aircraft vertical flights through clouds have been conducted. This new Return Glider Radiosonde (RG-R) is lifted up with double balloon technique to keep the radiation instruments as horizontal as possible during ascent. The RG-R is equipped with a routine radiosonde to transmit the data to a ground station and an autopilot to fly the glider radiosonde back to the launch site, where it lands autonomous with a parachute. The RG-R was successfully tested and deployed for tropospheric and stratospheric radiation measurements up to 30 hPa (24 km altitude) at the GRUAN sites Payerne (Switzerland) and Sodankylä (Finland). Radiation profiles and the radiation budget through the atmosphere during different daytimes and under cloud-free and cloudy situations will be shown in relation to temperature and humidity at the surface and in the atmosphere. The RG-R flight characteristics and new measurement possibilities will also be discussed.

A Flight Dynamics Model for a Small Glider in Ambient Winds

NASA Technical Reports Server (NTRS)

Beeler, Scott C.; Moerder, Daniel D.; Cox, David E.

2003-01-01

In this paper we describe the equations of motion developed for a point-mass zero-thrust (gliding) aircraft model operating in an environment of spatially varying atmospheric winds. The wind effects are included as an integral part of the flight dynamics equations, and the model is controlled through the three aerodynamic control angles. Formulas for the aerodynamic coefficients for this model are constructed to include the effects of several different aspects contributing to the aerodynamic performance of the vehicle. Characteristic parameter values of the model are compared with those found in a different set of small glider simulations. We execute a set of example problems which solve the glider dynamics equations to find the aircraft trajectory given specified control inputs. The ambient wind conditions and glider characteristics are varied to compare the simulation results under these different circumstances.

A Flight Dynamics Model for a Small Glider in Ambient Winds

NASA Technical Reports Server (NTRS)

Beeler, Scott C.; Moerder, Daniel D.; Cox, David E.

2003-01-01

In this paper we describe the equations of motion developed for a point-mass zero-thrust (gliding) aircraft model operating in an environment of spatially varying atmospheric winds. The wind effects are included as an integral part of the flight dynamics equations, and the model is controlled through the three aerodynamic control angles. Formulas for the aerodynamic coefficients for this model are constructed to include the effects of several different aspects contributing to the aerodynamic performance of the vehicle. Characteristic parameter values of the model are compared with those found in a different set of small glider simulations. We execute a set of example problems which solve the glider dynamics equations to find aircraft trajectory given specified control inputs. The ambient wind conditions and glider characteristics are varied to compare the simulation results under these different circumstances.

Duodenoscope hang time does not correlate with risk of bacterial contamination.

PubMed

Heroux, Riley; Sheppard, Michelle; Wright, Sharon B; Sawhney, Mandeep; Hirsch, Elizabeth B; Kalaidjian, Robin; Snyder, Graham M

2017-04-01

Current professional guidelines recommend a maximum hang time for reprocessed duodenoscopes of 5-14 days. We sought to study the association between hang time and risk of duodenoscope contamination. We analyzed cultures of the elevator mechanism and working channel collected in a highly standardized fashion just before duodenoscope use. Hang time was calculated as the time from reprocessing to duodenoscope sampling. The relationship between hang time and duodenoscope contamination was estimated using a calculated correlation coefficient between hang time in days and degree of contamination on the elevator mechanism and working channel. The 18 study duodenoscopes were cultured 531 times, including 465 (87.6%) in the analysis dataset. Hang time ranged from 0.07-39.93 days, including 34 (7.3%) with hang time ≥7.00 days. Twelve cultures (2.6%) demonstrated elevator mechanism and/or working channel contamination. The correlation coefficients for hang time and degree of duodenoscope contamination were very small and not statistically significant (-0.0090 [P = .85] for elevator mechanism and -0.0002 [P = 1.00] for working channel). Odds ratios for hang time (dichotomized at ≥7.00 days) and elevator mechanism and/or working channel contamination were not significant. We did not find a significant association between hang time and risk of duodenoscope contamination. Future guidelines should consider a recommendation of no limit for hang time. Copyright © 2017 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

Suicidal hanging donors for lung transplantation

PubMed Central

Ananiadou, Olga; Schmack, Bastian; Zych, Bartlomiej; Sabashnikov, Anton; Garcia-Saez, Diana; Mohite, Prashant; Weymann, Alexander; Mansur, Ashham; Zeriouh, Mohamed; Marczin, Nandor; De Robertis, Fabio; Simon, Andre Rüdiger; Popov, Aron-Frederik

2018-01-01

Abstract In the context of limited donor pool in cardiothoracic transplantation, utilization of organs from high risk donors, such as suicidal hanging donors, while ensuring safety, is under consideration. We sought to evaluate the outcomes of lung transplantations (LTx) that use organs from this group. Between January 2011 and December 2015, 265 LTx were performed at our center. Twenty-two recipients received lungs from donors after suicidal hanging (group 1). The remaining 243 transplantations were used as a control (group 2). Analysis of recipient and donor characteristics as well as outcomes was performed. No statistically significant difference was found in the donor characteristics between analyzed groups, except for higher incidence of cardiac arrest, younger age and smoking history of hanging donors (P < .001, P = .022 and P = .0042, respectively). Recipient preoperative and perioperative characteristics were comparable. Postoperatively in group 1 there was a higher incidence of extracorporeal life support (27.3 vs 9.1%, P = .019). There were no significant differences in chronic lung allograft dysfunction-free survival between group 1 and 2: 92.3 vs 94% at 1 year and 65.9 vs 75.5% at 3 years (P = .99). The estimated cumulative survival rate was also similar between groups: 68.2 vs 83.2% at 1 year and 68.2% versus 72% at 3 years (P = .3758). Hanging as a donor cause of death is not associated with poor mid-term survival or chronic lung allograft dysfunction following transplantation. These results encourage assessment of lungs from hanging donors, and their consideration for transplantation. PMID:29620623

Course of Near-hanging Victims Succumbed to Death: A Seven Year Study

PubMed Central

Mugadlimath, Anand B.; Zine, K.U.; Farooqui, Jamebaseer M.; Phalke, Balaji J.

2015-01-01

Introduction: Near hanging refers to victims who survive a hanging injury following attempted hanging, long enough to reach hospital. Delayed deaths in near hanging patients are mostly due to complication of hanging. The purpose of this study was to evaluate the demographics, mortality patterns and cause of delayed deaths in near hanging victims. Materials and Methods: In this study autopsy files over a seven year period from 2007 to 2013 were reviewed, and data of near hanging deaths (attempted hanging cases who succumbed to death and subjected for medicolegal autopsy) was extracted. Records of 14,000 autopsies was reviewed, and 10 deceased having died delayed deaths after near hanging episode were identified. In each case, the patients’ details, including gender, age, type of suspension, type of ligature material used for hanging and subsequent hanging mark produced were reviewed using autopsy reports and photographs taken during autopsy. Results: Demographic and pathological aspects of the each case discussed to throw light on autopsy findings in victims who died following near hanging. Complete suspension was present in 3 cases, while partial suspension was present in 7 cases. Survivals in delayed death after near hanging episode have ranged from 9 h to 72 d. Hypoxic encephalopathy was the most common cause of death, followed by pneumonia. Conclusion: Most of the near hanging patients did succumb to hypoxic encephalopathy; however, consolidation of lungs (pneumonia) was the next common cause of death reflecting need for aggressive oxygen therapy and selective resuscitation should be performed in all such cases. PMID:25954634

Accident rates for novice glider pilots vs. pilots with experience.

PubMed

Jarvis, Steve; Harris, Don

2007-12-01

It is a popular notion in gliding that newly soloed pilots have a low accident rate. The intention of this study was to review the support for such a hypothesis from literature and to explore it using UK accident totals and measures of flying exposure. Log sheets from UK gliding clubs were used to estimate flying exposure for inexperienced glider pilots. This was used along with accident data and annual flight statistics for the period 2004-2006 in order to estimate accident rates that could be compared between the pilot groups. The UK accident rate for glider pilots from 2004-2006 was 1 accident in every 3534 launches and 1590 flying hours. The lowest estimated rate for pilots with up to 1 h of experience was 1 accident every 976 launches and 149 h flown. For pilots with up to 10 h of experience the figures were 1 accident in 1274 launches and 503 h. From 2004-2006 UK glider pilots with 10 h or less experience in command had twice the number of accidents per launch and three times as many accidents per hour flown than average for UK glider pilots. Pilots with only 1 h of experience or less were involved in at least 10 times the number of accidents per hour flown than the UK average and had more than 3.5 times the number of accidents per launch.

77 FR 2674 - Airworthiness Directives; Glasflugel Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-19

... Models Standard Libelle-201B, Club Libelle 205, Mosquito, and Kestrel gliders. This proposed AD results... 205, all S/Ns (2) Kestrel, all S/Ns, except S/N 85, 110, and 125 (3) Mosquito, all S/Ns (4) Standard...

Game of life on phyllosilicates: Gliders, oscillators and still life

NASA Astrophysics Data System (ADS)

Adamatzky, Andrew

2013-10-01

A phyllosilicate is a sheet of silicate tetrahedra bound by basal oxygens. A phyllosilicate automaton is a regular network of finite state machines - silicon nodes and oxygen nodes - which mimics structure of the phyllosilicate. A node takes states 0 and 1. Each node updates its state in discrete time depending on a sum of states of its three (silicon) or six (oxygen) neighbours. Phyllosilicate automata exhibit localisations attributed to Conway's Game of Life: gliders, oscillators, still lifes, and a glider gun. Configurations and behaviour of typical localisations, and interactions between the localisations are illustrated.

Vertical suspended sediment fluxes observed from a formation of underwater gliders

NASA Astrophysics Data System (ADS)

Merckelbach, Lucas; Riethmueller, Rolf

2014-05-01

In order to understand and predict the pathways and deposition of fine sediments in coastal regions valid parameterisations of the fluxes across interfaces (sea bed - water column or a pycnocline) are paramount. Traditionally, these parameterisations are based on the concept of a critical shear stress, but more recently a probabilistic approach has been proposed, in which the resuspension of sediment is assumed to have a certain likelihood, depending on the external forcing. Both approaches find their justification, to some extent, from the results of laboratory experiments, however, in-situ data, essential for model validation, are scarce. In this study we develop a field method to estimate the (fine) sediment fluxes between the seabed and the water column, and across the pycnocline. The method is applied to a stratified shallow sea (the North Sea in Summer). In order to assess the results, these fluxes are interpreted in terms of bottom shear stress and current shear between upper and lower layer, respectively. The method was tested in an experiment with two underwater gliders in Summer 2013 in the German Bight. Both gliders were equipped with optical backscatter sensors, the measurements of which serve as a proxy for suspended sediment concentration. The profiling character of the gliders allows to calculate the rate of change of the layer-averaged sediment concentration, as observed by the platform. The local, Lagrangian rate of change of sediment concentration is the balance between the fluxes across the layer's interfaces. Due to a horizontal speed of the glider of about 0.5 m/s, horizontal gradients in sediment concentration cause the observed and the local rate of change of sediment concentration to be significantly different. The novelty of this experiment was that the two gliders were flown in a rigid formation, where one glider trailed the other at a more or less constant distance of 5 km, controlled by an algorithm. This allowed the local rate of change

Glider and Vision: two new families of miniature inverted-repeat transposable elements in Xenopus laevis genome.

PubMed

Lepetit, D; Pasquet, S; Olive, M; Thézé, N; Thiébaud, P

2000-01-01

We have characterised from Xenopus laevis two new short interspersed repetitive elements, we have named Glider and Vision, that belong to the family of miniature inverted-repeat transposable elements (MITEs). Glider was first characterised in an intronic region of the alpha-tropomyosin (alpha-TM) gene and database search has revealed the presence of this element in 10 other Xenopus laevis genes. Glider elements are about 150 bp long and for some of them, their terminal inverted repeats are flanked by potential target-site duplications. Evidence for the mobility of Glider element has been provided by the presence/absence of one element at corresponding location in duplicated alpha-TM genes. Vision element has been identified in the promoter region of the cyclin dependant kinase 2 gene (cdk2) where it is boxed in a Glider element. Vision is 284bp long and is framed by 14-bp terminal inverted repeats that are flanked by 7-bp direct repeats. We have estimated that there are about 20,000 and 300 copies of Glider and Vision respectively scattered throughout the Xenopus laevis genome. Every MITEs elements but two described in our study are found either in 5' or in 3' regulatory regions of genes suggesting a potential role in gene regulation.

A climatology of the California Current System from a network of underwater gliders

NASA Astrophysics Data System (ADS)

Rudnick, Daniel L.; Zaba, Katherine D.; Todd, Robert E.; Davis, Russ E.

2017-05-01

Autonomous underwater gliders offer the possibility of sustained observation of the coastal ocean. Since 2006 Spray underwater gliders in the California Underwater Glider Network (CUGN) have surveyed along California Cooperative Oceanic Fisheries Investigations (CalCOFI) lines 66.7, 80.0, and 90.0, constituting the world's longest sustained glider network, to our knowledge. In this network, gliders dive between the surface and 500 m, completing a cycle in 3 h and covering 3 km in that time. Sections extend 350-500 km offshore and take 2-3 weeks to occupy. Measured variables include pressure, temperature, salinity, and depth-average velocity. The CUGN has amassed over 10,000 glider-days, covering over 210,000 km with over 95,000 dives. These data are used to produce a climatology whose products are for each variable a mean field, an annual cycle, and the anomaly from the annual cycle. The analysis includes a weighted least-squares fit to derive the mean and annual cycle, and an objective map to produce the anomaly. The final results are variables on rectangular grids in depth, distance offshore, and time. The mean fields are finely resolved sections across the main flows in the California Current System, including the poleward California Undercurrent and the equatorward California Current. The annual cycle shows a phase change from the surface to the thermocline, reflecting the effects of air/sea fluxes at the surface and upwelling in the thermocline. The interannual anomalies are examined with an emphasis on climate events of the last ten years including the 2009-2010 El Niño, the 2010-2011 La Niña, the warm anomaly of 2014-2015, and the 2015-2016 El Niño.

3-D simulation of hanging wall effect at dam site

NASA Astrophysics Data System (ADS)

Zhang, L.; Xu, Y.

2017-12-01

Hanging wall effect is one of the near fault effects. This paper focuses on the difference of the ground motions on the hanging wall side between the footwall side of the fault at dam site considering the key factors, such as actual topography, the rupture process. For this purpose, 3-D ground motions are numerically simulated by the spectrum element method (SEM), which takes into account the physical mechanism of generation and propagation of seismic waves. With the SEM model of 548 million DOFs, excitation and propagation of seismic waves are simulated to compare the difference between the ground motion on the hanging wall side and that on the footwall side. Take Dagangshan region located in China as an example, several seismogenic finite faults with different dip angle are simulated to investigate the hanging wall effect. Furthermore, by comparing the ground motions of the receiving points, the influence of several factors on hanging wall effect is investigated, such as the dip of the fault and the fault type (strike slip fault or dip-slip fault). The peak acceleration on the hanging wall side is obviously larger than those on the footwall side, which numerically evidences the hanging wall effect. Besides, the simulation shows that only when the dip is less than 70° does the hanging wall effect deserve attention.

A Comparison of the Pac-X Trans-Pacific Wave Glider Data and Satellite Data (MODIS, Aquarius, TRMM and VIIRS)

PubMed Central

Villareal, Tracy A.; Wilson, Cara

2014-01-01

Four wave-propelled autonomous vehicles (Wave Gliders) instrumented with a variety of oceanographic and meteorological sensors were launched from San Francisco, CA in November 2011 for a trans-Pacific (Pac-X) voyage to test platform endurance. Two arrived in Australia, one in Dec 2012 and one in February 2013, while the two destined for Japan both ran into technical difficulties and did not arrive at their destination. The gliders were all equipped with sensors to measure temperature, salinity, turbidity, oxygen, and both chlorophyll and oil fluorescence. Here we conduct an initial assessment of the data set, noting necessary quality control steps and instrument utility. We conduct a validation of the Pac-X dataset by comparing the glider data to equivalent, or near-equivalent, satellite measurements. Sea surface temperature and salinity compared well to satellite measurements. Chl fluorescence from the gliders was more poorly correlated, with substantial between glider variability. Both turbidity and oil CDOM sensors were compromised to some degree by interfering processes. The well-known diel cycle in chlorophyll fluorescence was observed suggesting that mapping physiological data over large scales is possible. The gliders captured the Pacific Ocean’s major oceanographic features including the increased chlorophyll biomass of the California Current and equatorial upwelling. A comparison of satellite sea surface salinity (Aquarius) and glider-measured salinity revealed thin low salinity lenses in the southwestern Pacific Ocean. One glider survived a direct passage through a tropical cyclone. Two gliders traversed an open ocean phytoplankton bloom; extensive spiking in the chlorophyll fluorescence data is consistent with aggregation and highlights another potential future use for the gliders. On long missions, redundant instrumentation would aid in interpreting unusual data streams, as well as a means to periodically image the sensor heads. Instrument placement

A comparison of the Pac-X trans-Pacific Wave Glider data and satellite data (MODIS, Aquarius, TRMM and VIIRS).

PubMed

Villareal, Tracy A; Wilson, Cara

2014-01-01

Four wave-propelled autonomous vehicles (Wave Gliders) instrumented with a variety of oceanographic and meteorological sensors were launched from San Francisco, CA in November 2011 for a trans-Pacific (Pac-X) voyage to test platform endurance. Two arrived in Australia, one in Dec 2012 and one in February 2013, while the two destined for Japan both ran into technical difficulties and did not arrive at their destination. The gliders were all equipped with sensors to measure temperature, salinity, turbidity, oxygen, and both chlorophyll and oil fluorescence. Here we conduct an initial assessment of the data set, noting necessary quality control steps and instrument utility. We conduct a validation of the Pac-X dataset by comparing the glider data to equivalent, or near-equivalent, satellite measurements. Sea surface temperature and salinity compared well to satellite measurements. Chl fluorescence from the gliders was more poorly correlated, with substantial between glider variability. Both turbidity and oil CDOM sensors were compromised to some degree by interfering processes. The well-known diel cycle in chlorophyll fluorescence was observed suggesting that mapping physiological data over large scales is possible. The gliders captured the Pacific Ocean's major oceanographic features including the increased chlorophyll biomass of the California Current and equatorial upwelling. A comparison of satellite sea surface salinity (Aquarius) and glider-measured salinity revealed thin low salinity lenses in the southwestern Pacific Ocean. One glider survived a direct passage through a tropical cyclone. Two gliders traversed an open ocean phytoplankton bloom; extensive spiking in the chlorophyll fluorescence data is consistent with aggregation and highlights another potential future use for the gliders. On long missions, redundant instrumentation would aid in interpreting unusual data streams, as well as a means to periodically image the sensor heads. Instrument placement is

Glider Flight Instructor Written Test Guide.

ERIC Educational Resources Information Center

Federal Aviation Administration (DOT), Washington, DC. Flight Standards Service.

The purposes of the test guide are threefold. First, it is intended to outline the scope of the basic aeronautical knowledge requirements for a glider flight instructor. This includes fundamentals of flight instruction and performance and analysis of flight training maneuvers. Secondly, it is intended to acquaint the applicant with source material…

A novel technique for orchiectomy and scrotal ablation in the sugar glider (Petaurus breviceps).

PubMed

Morges, Michelle A; Grant, Krystan R; MacPhail, Catriona M; Johnston, Matthew S

2009-03-01

In this report, we describe a simple, safe, and efficacious technique for orchiectomy and scrotal ablation with the use of a carbon dioxide light amplification by stimulated emission of radiation (laser) in sugar gliders (Petaurus breviceps). The study population included nine sugar gliders, ages 2-24 mo, presented for orchiectomy. After induction of general anesthesia, orchiectomy and scrotal ablation were performed by severing the scrotal stalk with the laser. All sugar gliders were discharged the same day. Mean anesthesia time was 6.09 +/- 0.94 min. Mean surgery time was 15.11 +/- 8.39 sec, and mean recovery time was 4.11 +/- 3.60 min. With an outlier removed, mean recovery time was 2.95 +/- 1.03 min. No serious postoperative surgical complications were observed. The technique described is a simple procedure with rapid surgical and recovery times that has the potential to become the standard procedure for orchiectomy and scrotal ablation in sugar gliders.

Assimilating bio-optical glider data during a phytoplankton bloom in the southern Ross Sea

NASA Astrophysics Data System (ADS)

Kaufman, Daniel E.; Friedrichs, Marjorie A. M.; Hemmings, John C. P.; Smith, Walker O., Jr.

2018-01-01

The Ross Sea is a region characterized by high primary productivity in comparison to other Antarctic coastal regions, and its productivity is marked by considerable variability both spatially (1-50 km) and temporally (days to weeks). This variability presents a challenge for inferring phytoplankton dynamics from observations that are limited in time or space, which is often the case due to logistical limitations of sampling. To better understand the spatiotemporal variability in Ross Sea phytoplankton dynamics and to determine how restricted sampling may skew dynamical interpretations, high-resolution bio-optical glider measurements were assimilated into a one-dimensional biogeochemical model adapted for the Ross Sea. The assimilation of data from the entire glider track using the micro-genetic and local search algorithms in the Marine Model Optimization Testbed improves the model-data fit by ˜ 50 %, generating rates of integrated primary production of 104 g C m-2 yr-1 and export at 200 m of 27 g C m-2 yr-1. Assimilating glider data from three different latitudinal bands and three different longitudinal bands results in minimal changes to the simulations, improves the model-data fit with respect to unassimilated data by ˜ 35 %, and confirms that analyzing these glider observations as a time series via a one-dimensional model is reasonable on these scales. Whereas assimilating the full glider data set produces well-constrained simulations, assimilating subsampled glider data at a frequency consistent with cruise-based sampling results in a wide range of primary production and export estimates. These estimates depend strongly on the timing of the assimilated observations, due to the presence of high mesoscale variability in this region. Assimilating surface glider data subsampled at a frequency consistent with available satellite-derived data results in 40 % lower carbon export, primarily resulting from optimized rates generating more slowly sinking diatoms. This

White River National Forest Hanging Lake Capacity Study

DOT National Transportation Integrated Search

2016-05-01

Due to overcrowding at Hanging Lake, the Volpe Center performed a capacity study for the White River National Forest. This capacity study examines the visitation and environmental constraints of Hanging Lake parking lot, trail, and site to develop th...

Agonal sequences in eight filmed hangings: analysis of respiratory and movement responses to asphyxia by hanging.

PubMed

Sauvageau, Anny; LaHarpe, Romano; Geberth, Vernon J

2010-09-01

It has been proposed that filmed hangings may hold the key to a better understanding of human asphyxia, and The Working Group on Human Asphyxia was formed to systematically review and compare these video recordings. This study analyzed eight filmed hangings. Considering time 0 to represent the onset of the final hanging, rapid loss of consciousness was observed (at 8-18 sec), closely followed by convulsions (at 10-19 sec). A complex pattern of decerebrate rigidity and decorticate rigidity then followed. Between 1 min 38 sec and 2 min 15 sec, muscle tone seemed to be lost, the body becoming progressively flaccid. From then on, isolated body movements were observed from time to time, the last one occurring between 1 min 2 sec and 7 min 31 sec. As for the respiratory responses, all cases presented deep rhythmic abdominal respiratory movements (last one between 1 min 2 sec and 2 min 5 sec). © 2010 American Academy of Forensic Sciences.

Characteristic Features of Hanging: A Study in Rural District of Central India.

PubMed

Ambade, Vipul Namdeorao; Kolpe, Dayanand; Tumram, Nilesh; Meshram, Satin; Pawar, Mohan; Kukde, Hemant

2015-09-01

The ligature mark is the most relevant feature of hanging. This study was undertaken with a view to determine the characteristic features of hanging and its association with ligature material or mode of suspension. Of a total medicolegal deaths reported at an Apex Medical Centre, hanging was noted in 4.1% cases, all suicidal with mortality rate of 1.5 per 100,000 population per year. The hanging was complete in 67.7% with nylon rope as the commonest type of ligature material used for ligation. The hanging mark was usually single, situated above thyroid cartilage, incomplete, prominent, and directed toward nape of neck. The mark of dribbling of saliva was seen in 11.8% cases. Facial congestion, petechial hemorrhage, and cyanosis were significantly seen in partial hanging. Though occasionally reported, the argent line was noted in 78.7% hanging deaths with neck muscle hemorrhage in 23.6% cases. Fracture of neck structure was predominant in complete hanging. © 2015 American Academy of Forensic Sciences.

Hands Tied with Bag Full of Books in Suicidal Hanging.

PubMed

Sikary, Asit Kumar; Behera, Chittaranjan; Murty, Om Prakash; Rautji, Ravi

2016-01-01

Hanging deaths associated with binding of limbs, masking of a face, and gagging are always suspicious. In suicidal hanging, the victim uses these added techniques to prevent him from backing out of his decision and to ensure death. However, binding of limbs and adding extra weight to the suspension in hanging are not reported. Herein, we report a case where the victim tied a bag containing books weighing 7 kg (15.4 lbs) to both his hands during hanging. The forensic specialist must be aware of the unusual presentation of suicidal hanging which may suggest foul play. The manner of death must be established after detailed analysis of circumstantial evidence, information obtained from the witnesses, complete autopsy, and toxicological examination. © 2015 American Academy of Forensic Sciences.

Using an autonomous Wave Glider to detect seawater anomalies related to submarine groundwater discharge - engineering challenge

NASA Astrophysics Data System (ADS)

Leibold, P.; Brueckmann, W.; Schmidt, M.; Balushi, H. A.; Abri, O. A.

2017-12-01

Coastal aquifer systems are amongst the most precious and vulnerable water resources worldwide. While differing in lateral and vertical extent they commonly show a complex interaction with the marine realm. Excessive groundwater extraction can cause saltwater intrusion from the sea into the aquifers, having a strongly negative impact on the groundwater quality. While the reverse pathway, the discharge of groundwater into the sea is well understood in principle, it's mechanisms and quantities not well constrained. We will present a project that combines onshore monitoring and modeling of groundwater in the coastal plain of Salalah, Oman with an offshore autonomous robotic monitoring system, the Liquid Robotics Wave Glider. Eventually, fluxes detected by the Wave Glider system and the onshore monitoring of groundwater will be combined into a 3-D flow model of the coastal and deeper aquifers. The main tool for offshore SGD investigation project is a Wave Glider, an autonomous vehicle based on a new propulsion technology. The Wave Glider is a low-cost satellite-connected marine craft, consisting of a combination of a sea-surface and an underwater component which is propelled by the conversion of ocean wave energy into forward thrust. While the wave energy propulsion system is purely mechanical, electrical energy for onboard computers, communication and sensors is provided by photovoltaic cells. For the project the SGD Wave Glider is being equipped with dedicated sensors to measure temperature, conductivity, Radon isotope (222Rn, 220Rn) activity concentration as well as other tracers of groundwater discharge. Dedicated software using this data input will eventually allow the Wave Glider to autonomously collect information and actively adapt its search pattern to hunt for spatial and temporal anomalies. Our presentation will focus on the engineering and operational challenges ofdetecting submarine groundwater discharges with the Wave Glider system in the Bay of Salalah

Wind tunnel investigations of glider fuselages with different waistings and wing arrangements

NASA Technical Reports Server (NTRS)

Radespiel, R.

1983-01-01

The parameters fuselage pinch glider wing arrangement and fuselage leading edge radius of nine glider configurations were investigated in wind tunnel tests. Laminar separation bubbles were found on strongly recessed fuselages. These separations in the juncture between fuselage and wing are essential in the prevention of harmful aerodynamic drag. Drag reduction was measured with increasing pinch and the wing arrangement in the rear. These results are only valid for laminar flow on the fuselage leading edge.

Gliders of World War II: ’The Bastards No One Wanted’

DTIC Science & Technology

2008-06-01

BIBLIOGRAPHY Ammerman, Gale R . "An American Glider Pilot’s Story." Air Power History vol 46 no 1 (1999): 18-24. Barringer , Lewin Bennitt. Flight without...comments helped guide the research effort. Next, I would equally like to thank Dr. Richard R . Muller for encouraging me to consider the glider’s role...Field, November 1947), 2, as referenced in Janet R . Daly Bednarek, “American Combat Glider Program, 1941-1947: “Damned Fool Idea”,” Air Power

Isolation and characterization of a novel type of rotavirus species A in sugar gliders (Petaurus breviceps).

PubMed

Okadera, Kota; Abe, Masako; Ito, Naoto; Mitake, Hiromichi; Okada, Kazuma; Nakagawa, Kento; Une, Yumi; Tsunemitsu, Hiroshi; Sugiyama, Makoto

2016-05-01

To estimate the risk of interspecies transmission of rotavirus species A (RVA) from exotic pets to other mammalian species, the prevalence of RVA in sugar gliders (Petaurus breviceps) was investigated. RVAs were detected in 10 of 44 sugar gliders by reverse transcription (RT)-semi-nested PCR. These viruses were classified as G27P[3] and G27P[36] genotypes, with G27 and P[36] being new genotypes as assigned by the Rotavirus Classification Working Group. To characterize sugar glider RVA in detail, one strain, RVA/SugarGlider-tc/JPN/SG385/2012/G27P[36] (SG385-tc), was isolated. All of the genes of the strain were classified as new genotypes (G27-P[36]-I19-R10-C10-M9-A20-N11-T13-E17-H12). The enterotoxin domain in NSP4, which is important for the induction of diarrhoea, was conserved between SG385-tc and previously reported mammalian strains, suggesting the potential of sugar glider RVA to cause diarrhoea in mammalian species. In fact, seven out of nine suckling mice inoculated orally with 3.9 × 104 f.f.u. of strain SG385-tc had diarrhoea and the 50 % diarrhoea-inducing dose (DD50) of strain SG385-tc in suckling mice was 1.2 × 104 f.f.u. Our findings suggest that sugar glider RVA is infective to and possibly pathogenic in other mammalian species.

76 FR 23169 - Airworthiness Directives; DG Flugzeugbau GmbH Glaser-Dirks Model DG-808C Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-26

... Directives; DG Flugzeugbau GmbH Glaser-Dirks Model DG-808C Gliders AGENCY: Federal Aviation Administration... applies to DG Flugzeugbau GmbH Glaser-Dirks Models DG-808C gliders, serial numbers 8-316 B 216 X 1 through...

Return glider radiosonde for in situ upper-air research measurements

NASA Astrophysics Data System (ADS)

Kräuchi, Andreas; Philipona, Rolf

2016-06-01

Upper-air balloon soundings for weather predictions have been made since the beginning of the 20th century. New radiosonde instruments for in situ humidity-, radiation- and gas-profile measurements in the troposphere and the lower stratosphere, were introduced in recent years for atmospheric research and climate monitoring, but such instruments are often expensive and it is desired they be reused on multiple flights. Recovering instruments that freely descend with parachutes is time consuming, sometimes difficult and even dangerous. Here, we introduce the return glider radiosonde (RGR), which enables flying and retrieving valuable in situ upper-air instruments. The RGR is lifted with weather balloons similar to traditional radiosondes to a preset altitude, at which time a release mechanism cuts the tether string, and a built-in autopilot flies the glider autonomously back to the launch site or a desired preprogrammed location. Once the RGR reaches the landing coordinates it circles down and releases a parachute 100 m above ground for landing. The motivation for this project was to measure radiation profiles throughout the atmosphere with the same instrument multiple times and with a rapid turn-around time. The paper describes technical aspects of the return glider radiosonde and the built-in radiation instruments and shows test flights up to 24 km altitude that are analyzed in terms of flight performance and maximal distances covered. Several successive flights measuring radiation profiles demonstrate the reliability and the operational readiness of the RGR, allowing new ways for atmospheric in situ research and monitoring with payloads up to several kg depending on the specific size of the glider.

Mission Planning and Decision Support for Underwater Glider Networks: A Sampling on-Demand Approach

PubMed Central

Ferri, Gabriele; Cococcioni, Marco; Alvarez, Alberto

2015-01-01

This paper describes an optimal sampling approach to support glider fleet operators and marine scientists during the complex task of planning the missions of fleets of underwater gliders. Optimal sampling, which has gained considerable attention in the last decade, consists in planning the paths of gliders to minimize a specific criterion pertinent to the phenomenon under investigation. Different criteria (e.g., A, G, or E optimality), used in geosciences to obtain an optimum design, lead to different sampling strategies. In particular, the A criterion produces paths for the gliders that minimize the overall level of uncertainty over the area of interest. However, there are commonly operative situations in which the marine scientists may prefer not to minimize the overall uncertainty of a certain area, but instead they may be interested in achieving an acceptable uncertainty sufficient for the scientific or operational needs of the mission. We propose and discuss here an approach named sampling on-demand that explicitly addresses this need. In our approach the user provides an objective map, setting both the amount and the geographic distribution of the uncertainty to be achieved after assimilating the information gathered by the fleet. A novel optimality criterion, called Aη, is proposed and the resulting minimization problem is solved by using a Simulated Annealing based optimizer that takes into account the constraints imposed by the glider navigation features, the desired geometry of the paths and the problems of reachability caused by ocean currents. This planning strategy has been implemented in a Matlab toolbox called SoDDS (Sampling on-Demand and Decision Support). The tool is able to automatically download the ocean fields data from MyOcean repository and also provides graphical user interfaces to ease the input process of mission parameters and targets. The results obtained by running SoDDS on three different scenarios are provided and show that So

Mission Planning and Decision Support for Underwater Glider Networks: A Sampling on-Demand Approach.

PubMed

Ferri, Gabriele; Cococcioni, Marco; Alvarez, Alberto

2015-12-26

This paper describes an optimal sampling approach to support glider fleet operators and marine scientists during the complex task of planning the missions of fleets of underwater gliders. Optimal sampling, which has gained considerable attention in the last decade, consists in planning the paths of gliders to minimize a specific criterion pertinent to the phenomenon under investigation. Different criteria (e.g., A, G, or E optimality), used in geosciences to obtain an optimum design, lead to different sampling strategies. In particular, the A criterion produces paths for the gliders that minimize the overall level of uncertainty over the area of interest. However, there are commonly operative situations in which the marine scientists may prefer not to minimize the overall uncertainty of a certain area, but instead they may be interested in achieving an acceptable uncertainty sufficient for the scientific or operational needs of the mission. We propose and discuss here an approach named sampling on-demand that explicitly addresses this need. In our approach the user provides an objective map, setting both the amount and the geographic distribution of the uncertainty to be achieved after assimilating the information gathered by the fleet. A novel optimality criterion, called A η , is proposed and the resulting minimization problem is solved by using a Simulated Annealing based optimizer that takes into account the constraints imposed by the glider navigation features, the desired geometry of the paths and the problems of reachability caused by ocean currents. This planning strategy has been implemented in a Matlab toolbox called SoDDS (Sampling on-Demand and Decision Support). The tool is able to automatically download the ocean fields data from MyOcean repository and also provides graphical user interfaces to ease the input process of mission parameters and targets. The results obtained by running SoDDS on three different scenarios are provided and show that So

The Hanging Cord with a Real Tip Mass

ERIC Educational Resources Information Center

Deschaine, J. S.; Suits, B. H.

2008-01-01

Normal mode solutions for the perfectly flexible hanging cord problem have been known for over 200 years. More recently, theoretical results for a hanging cord with a point mass attached were presented. Here the theoretical results are tested experimentally using high-precision techniques which are accessible for use in an introductory laboratory.…

The X-1 Paper Glider Kit: Investigating the Basics of Flight with a Model of the First Supersonic Aircraft. Educational Brief.

ERIC Educational Resources Information Center

National Aeronautics and Space Administration, Washington, DC.

This brief discusses the basics of flight using a model of the first supersonic aircraft. This activity for grades 5-12 has students build a glider and learn how to change the flight characteristics of the glider. A template for the glider is included. (MVL)

36 CFR 327.4 - Aircraft.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., helicopters, ultra-light aircraft, motorized hang gliders, hot air balloons, any non-powered flight devices or..., material or equipment by parachute, balloon, helicopter or other means onto or from project lands or waters...

BILATERAL HYDRONEPHROSIS IN A SUGAR GLIDER (PETAURUS BREVICEPS).

PubMed

Cusack, Lara; Schnellbacher, Rodney; Howerth, Elizabeth W; Jiménez, David A; Mayer, Joerg; Divers, Stephen

2016-09-01

An adult, intact male sugar glider ( Petaurus breviceps ) presented for acute caudal abdominal swelling. Treatment by the referring veterinarian included aspiration of urine from the swelling. On physical examination, mild depression, pale mucus membranes, and caudal abdominal swelling were noted. Focused ultrasonographic assessment revealed a fluid-filled caudal abdominal structure and subjective bladder wall thickening. The following day, the sugar glider was severely depressed. Hematology results included hypoglycemia, hyperkalemia, hyponatremia, and azotemia. Ultrasonography revealed bilateral hydronephrosis and hydroureter. Despite supportive care, the animal died. Postmortem examination confirmed bilateral ureteral dilation, renal petechial hemorrhage, and dilation of the right renal pelvis. Submucosal edema, hemorrhage, and lymphoplasmacytic infiltration of the urinary bladder, ureters, and renal pelvises were noted. Hyperplasia of the urinary bladder and ureteral epithelium, coupled with inflammation, may have caused functional obstruction leading to bilateral hydronephrosis and hydroureter. This is the first reported case of hydronephrosis in a marsupial.

The scotopic electroretinogram of the sugar glider related to histological features of its retina.

PubMed

Akula, James D; Esdaille, Tricia M; Caffé, A Romeo; Naarendorp, Franklin

2011-11-01

The flash electroretinogram (ERG) was used to characterize the scotopic retinal function in a marsupial. Key parameter values of the a- and b-waves of adult male sugar gliders, Petaurus breviceps breviceps, elicited with ganzfeld flashes were determined under dark- and light-adapted conditions. Using standard histological methods, the thicknesses of the major layers of the retina were assessed to provide insight into the nature of the ERG responses. The ERG and histological results were compared to corresponding data for placental C57Bl/6 mice to establish whether the functional retinal specialization that underlies scotopic visual function in a marsupial parallels that of a placental mouse. The sensitivity of the a-wave assessed with the Lamb and Pugh (Invest Ophthalmol Vis Sci 47:5138-5152, 2006) "model" and that of the b-wave assessed with standard methods were lower in the sugar glider compared to the mouse. The thickness of the sugar glider retina was two-third of that of the mouse. The high-intensity flash ERG of the sugar glider substantially differed in shape from that of the mouse reflecting perhaps structural and functional differences between the two species at the level of the inner retina.

Fabrication and Operation of Microfluidic Hanging-Drop Networks.

PubMed

Misun, Patrick M; Birchler, Axel K; Lang, Moritz; Hierlemann, Andreas; Frey, Olivier

2018-01-01

The hanging-drop network (HDN) is a technology platform based on a completely open microfluidic network at the bottom of an inverted, surface-patterned substrate. The platform is predominantly used for the formation, culturing, and interaction of self-assembled spherical microtissues (spheroids) under precisely controlled flow conditions. Here, we describe design, fabrication, and operation of microfluidic hanging-drop networks.

Acute pulmonary emphysema in death by hanging: a morphometric digital study.

PubMed

Castiglioni, Claudia; Baumann, Pia; Fracasso, Tony

2016-09-01

Acute pulmonary emphysema (APE) has been described in cases of mechanical asphyxia such as ligature or manual strangulation but not in cases of hanging. In this study, we wanted to verify by morphometric digital analysis of lung tissue whether APE occurs in death by hanging.We investigated 16 cases of hanging (eight complete, eight incomplete), 10 cases of freshwater drowning (positive control group), and 10 cases of acute external bleeding (negative control group). Tissue sections were obtained from each pulmonary lobe. For each slide, five fields were randomly selected. The area of every alveolar space was measured by image analysis software. The mean alveolar area (MAA) was calculated for each group.In incomplete hanging, MAA was significantly higher than that observed in complete hanging and similar to the one observed in freshwater drowning.APE in cases of incomplete hanging can be considered as a sign of vitality. The high number of conditions that can cause alveolar distension (that were excluded in this study) limits the applicability of this vital sign in the routine forensic practice.

77 FR 55411 - Airworthiness Directives; Glasflugel Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-10

... airworthiness directive (AD) for Glasflugel Models Standard Libelle-201B, Club Libelle 205, Mosquito, and... Standard Libelle-201B, Club Libelle 205, Mosquito, and Kestrel gliders. That AD resulted from mandatory..., except S/N 85, 110, and 125. (3) Mosquito, all S/Ns. (4) Standard Libelle-201B, S/N 169. (d) Subject Air...

The Tool for Designing Engineering Systems Using a New Optimization Method Based on a Stochastic Process

NASA Astrophysics Data System (ADS)

Yoshida, Hiroaki; Yamaguchi, Katsuhito; Ishikawa, Yoshio

The conventional optimization methods were based on a deterministic approach, since their purpose is to find out an exact solution. However, these methods have initial condition dependence and risk of falling into local solution. In this paper, we propose a new optimization method based on a concept of path integral method used in quantum mechanics. The method obtains a solutions as an expected value (stochastic average) using a stochastic process. The advantages of this method are not to be affected by initial conditions and not to need techniques based on experiences. We applied the new optimization method to a design of the hang glider. In this problem, not only the hang glider design but also its flight trajectory were optimized. The numerical calculation results showed that the method has a sufficient performance.

Design Tool Using a New Optimization Method Based on a Stochastic Process

NASA Astrophysics Data System (ADS)

Yoshida, Hiroaki; Yamaguchi, Katsuhito; Ishikawa, Yoshio

Conventional optimization methods are based on a deterministic approach since their purpose is to find out an exact solution. However, such methods have initial condition dependence and the risk of falling into local solution. In this paper, we propose a new optimization method based on the concept of path integrals used in quantum mechanics. The method obtains a solution as an expected value (stochastic average) using a stochastic process. The advantages of this method are that it is not affected by initial conditions and does not require techniques based on experiences. We applied the new optimization method to a hang glider design. In this problem, both the hang glider design and its flight trajectory were optimized. The numerical calculation results prove that performance of the method is sufficient for practical use.

Seasonal changes in the thermoenergetics of the marsupial sugar glider, Petaurus breviceps.

PubMed

Holloway, J C; Geiser, F

2001-11-01

Little information is available on seasonal changes in thermal physiology and energy expenditure in marsupials. To provide new information on the subject, we quantified how body mass, body composition, metabolic rate, maximum heat production, body temperature and thermal conductance change with season in sugar gliders (Petaurus breviceps) held in outdoor aviaries. Sugar gliders increased body mass in autumn to a peak in May/June, which was caused to a large extent by an increase in body fat content. Body mass then declined to minimum values in August/September. Resting metabolic rate both below and above the thermoneutral zone (TNZ) was higher in summer than in winter and the lower critical temperature of the TNZ occurred at a higher ambient temperature (Ta) in summer. The basal metabolic rate was as much as 45% below that predicted from allometric equations for placental mammals and was about 15% lower in winter than in summer. In contrast, maximum heat production was raised significantly by about 20% in winter. This, together with an approximately 20% decrease in thermal conductance, resulted in a 13 degrees C reduction of the minimum effective Ta gliders were able to withstand. Our study provides the first evidence that, despite the apparent lack of functional brown adipose tissue, sugar gliders are able to significantly increase heat production in winter. Moreover, the lower thermoregulatory heat production at most TaS in winter, when food in the wild is scarce, should allow them to reduce energy expenditure.

The Wave Glider°: A New Autonomous Surface Vehicle to Augment MBARI's Growing Fleet of Ocean Observing Systems

NASA Astrophysics Data System (ADS)

Tougher, B. B.

2011-12-01

Monterey Bay Aquarium Research Institute's (MBARI) evolving fleet of ocean observing systems has made it possible to collect information and data about a wide variety of ocean parameters, enabling researchers to better understand marine ecosystems. In collaboration with Liquid Robotics Inc, the designer of the Wave Glider autonomous surface vehicle (ASV), MBARI is adding a new capability to its suite of ocean observing tools. This new technology will augment MBARI research programs that use satellites, ships, moorings, drifters, autonomous underwater vehicles (AUVs) and remotely operated vehicles (ROVs) to improve data collection of temporally and spatially variable oceanographic features. The Wave Glider ASV derives its propulsion from wave energy, while sensors and communications are powered through the use of two solar panels and batteries, enabling it to remain at sea indefinitely. Wave Gliders are remotely controlled via real-time Iridium burst communications, which also permit real-time data telemetry. MBARI has developed Ocean Acidification (OA) moorings to continuously monitor the chemical and physical changes occurring in the ocean as a result of increased levels of atmospheric carbon dioxide (CO2). The moorings are spatially restricted by being anchored to the seafloor, so during the summer of 2011 the ocean acidification sensor suite designed for moorings was integrated into a Wave Glider ASV to increase both temporal and spatial ocean observation capabilities. The OA sensor package enables the measurement of parameters essential to better understanding the changing acidity of the ocean, specifically pCO2, pH, oxygen, salinity and temperature. The Wave Glider will also be equipped with a meteorological sensor suite that will measure air temperature, air pressure, and wind speed and direction. The OA sensor integration into a Wave Glider was part of MBARI's 2011 summer internship program. This project involved designing a new layout for the OA sensors

Structural Differences in Gray Matter between Glider Pilots and Non-Pilots. A Voxel-Based Morphometry Study

PubMed Central

Ahamed, Tosif; Kawanabe, Motoaki; Ishii, Shin; Callan, Daniel E.

2014-01-01

Glider flying is a unique skill that requires pilots to control an aircraft at high speeds in three dimensions and amidst frequent full-body rotations. In the present study, we investigated the neural correlates of flying a glider using voxel-based morphometry. The comparison between gray matter densities of 15 glider pilots and a control group of 15 non-pilots exhibited significant gray matter density increases in left ventral premotor cortex, anterior cingulate cortex, and the supplementary eye field. We posit that the identified regions might be associated with cognitive and motor processes related to flying, such as joystick control, visuo-vestibular interaction, and oculomotor control. PMID:25506339

Structural Differences in Gray Matter between Glider Pilots and Non-Pilots. A Voxel-Based Morphometry Study.

PubMed

Ahamed, Tosif; Kawanabe, Motoaki; Ishii, Shin; Callan, Daniel E

2014-01-01

Glider flying is a unique skill that requires pilots to control an aircraft at high speeds in three dimensions and amidst frequent full-body rotations. In the present study, we investigated the neural correlates of flying a glider using voxel-based morphometry. The comparison between gray matter densities of 15 glider pilots and a control group of 15 non-pilots exhibited significant gray matter density increases in left ventral premotor cortex, anterior cingulate cortex, and the supplementary eye field. We posit that the identified regions might be associated with cognitive and motor processes related to flying, such as joystick control, visuo-vestibular interaction, and oculomotor control.

A multidisciplinary glider survey of an open ocean dead-zone eddy

NASA Astrophysics Data System (ADS)

Karstensen, Johannes; Schütte, Florian; Pietri, Alice; Krahmann, Gerd; Fiedler, Björn; Löscher, Carolin; Grundle, Damian; Hauss, Helena; Körtzinger, Arne; Testor, Pierre; Viera, Nuno

2016-04-01

The physical (temperature, salinity) and biogeochemical (oxygen, nitrate, chlorophyll fluorescence, turbidity) structure of an anticyclonic modewater eddy, hosting an open ocean dead zone, is investigated using observational data sampled in high temporal and spatial resolution with autonomous gliders in March and April 2014. The core of the eddy is identified in the glider data as a volume of fresher (on isopycnals) water in the depth range from the mixed layer base (about 70m) to about 200m depth. The width is about 80km. The core aligns well with the 40 μmolkg-1 oxygen contour. From two surveys about 1 month apart, changes in the minimal oxygen concentrations (below 5μmolkg-1) are observed that indicate that small scale processes are in operation. Several scales of coherent variability of physical and biogeochemical variable are identified - from a few meters to the mesoscale. One of the gliders carried an autonomous Nitrate (N) sensor and the data is used to analyse the possible nitrogen pathways within the eddy. Also the highest N is accompanied by lowest oxygen concentrations, the AOU:N ratio reveals a preferred oxygen cycling per N.

Dynamics of ultralight aircraft: Motion in vertical gusts

NASA Technical Reports Server (NTRS)

Jones, R. T.

1977-01-01

Gust load calculations are extended to the range of conditions encountered by ultralight aircraft such as hang gliders. Having wing loadings of the order of 5 kg/sq m, these gliders acquire a substantial fraction of the motion of a gust within a distance of 1 or 2 m. Comparative loads and displacements for a small powered airplane having a wing loading of 50 kg sq m and for a commercial jet with 500 kg sq m are shown.

Decomposition Rate and Pattern in Hanging Pigs.

PubMed

Lynch-Aird, Jeanne; Moffatt, Colin; Simmons, Tal

2015-09-01

Accurate prediction of the postmortem interval requires an understanding of the decomposition process and the factors acting upon it. A controlled experiment, over 60 days at an outdoor site in the northwest of England, used 20 freshly killed pigs (Sus scrofa) as human analogues to study decomposition rate and pattern. Ten pigs were hung off the ground and ten placed on the surface. Observed differences in the decomposition pattern required a new decomposition scoring scale to be produced for the hanging pigs to enable comparisons with the surface pigs. The difference in the rate of decomposition between hanging and surface pigs was statistically significant (p=0.001). Hanging pigs reached advanced decomposition stages sooner, but lagged behind during the early stages. This delay is believed to result from lower variety and quantity of insects, due to restricted beetle access to the aerial carcass, and/or writhing maggots falling from the carcass. © 2015 American Academy of Forensic Sciences.

Dynamic modeling and motion simulation for a winged hybrid-driven underwater glider

NASA Astrophysics Data System (ADS)

Wang, Shu-Xin; Sun, Xiu-Jun; Wang, Yan-Hui; Wu, Jian-Guo; Wang, Xiao-Ming

2011-03-01

PETREL, a winged hybrid-driven underwater glider is a novel and practical marine survey platform which combines the features of legacy underwater glider and conventional AUV (autonomous underwater vehicle). It can be treated as a multi-rigid-body system with a floating base and a particular hydrodynamic profile. In this paper, theorems on linear and angular momentum are used to establish the dynamic equations of motion of each rigid body and the effect of translational and rotational motion of internal masses on the attitude control are taken into consideration. In addition, due to the unique external shape with fixed wings and deflectable rudders and the dual-drive operation in thrust and glide modes, the approaches of building dynamic model of conventional AUV and hydrodynamic model of submarine are introduced, and the tailored dynamic equations of the hybrid glider are formulated. Moreover, the behaviors of motion in glide and thrust operation are analyzed based on the simulation and the feasibility of the dynamic model is validated by data from lake field trials.

Diversity-based acoustic communication with a glider in deep water.

PubMed

Song, H C; Howe, Bruce M; Brown, Michael G; Andrew, Rex K

2014-03-01

The primary use of underwater gliders is to collect oceanographic data within the water column and periodically relay the data at the surface via a satellite connection. In summer 2006, a Seaglider equipped with an acoustic recording system received transmissions from a broadband acoustic source centered at 75 Hz deployed on the bottom off Kauai, Hawaii, while moving away from the source at ranges up to ∼200 km in deep water and diving up to 1000-m depth. The transmitted signal was an m-sequence that can be treated as a binary-phase shift-keying communication signal. In this letter multiple receptions are exploited (i.e., diversity combining) to demonstrate the feasibility of using the glider as a mobile communication gateway.

Variable-Structure Control of a Model Glider Airplane

NASA Technical Reports Server (NTRS)

Waszak, Martin R.; Anderson, Mark R.

2008-01-01

A variable-structure control system designed to enable a fuselage-heavy airplane to recover from spin has been demonstrated in a hand-launched, instrumented model glider airplane. Variable-structure control is a high-speed switching feedback control technique that has been developed for control of nonlinear dynamic systems.

Hypersonic Glider Model in Full Scale Tunnel 1957

NASA Image and Video Library

1957-09-07

L57-1439 A model based on Langley s concept of a hypersonic glider was test flown on an umbilical cord inside the Full Scale Tunnel in 1957. Photograph published in Engineer in Charge: A History of the Langley Aeronautical Laboratory, 1917-1958 by James R. Hansen. Page 374.

Motion analysis and trials of the deep sea hybrid underwater glider Petrel-II

NASA Astrophysics Data System (ADS)

Liu, Fang; Wang, Yan-hui; Wu, Zhi-liang; Wang, Shu-xin

2017-03-01

A hybrid underwater glider Petrel-II has been developed and field tested. It is equipped with an active buoyancy unit and a compact propeller unit. Its working modes have been expanded to buoyancy driven gliding and propeller driven level-flight, which can make the glider work in strong currents, as well as many other complicated ocean environments. Its maximal gliding speed reaches 1 knot and the propelling speed is up to 3 knots. In this paper, a 3D dynamic model of Petrel-II is derived using linear momentum and angular momentum equations. According to the dynamic model, the spiral motion in the underwater space is simulated for the gliding mode. Similarly the cycle motion on water surface and the depth-keeping motion underwater are simulated for the level-flight mode. These simulations are important to the performance analysis and parameter optimization for the Petrel-II underwater glider. The simulation results show a good agreement with field trials.

Marginal Ice Zone: Biogeochemical Sampling with Gliders

DTIC Science & Technology

2015-09-30

chlorophyll primary productivity model to estimate and compare phytoplankton productivity under full ice cover, in the MIZ, and in open ice-free water...observing array (Fig. 1). The glider sensor suite included temperature, temperature microstructure, salinity, oxygen, chlorophyll fluorescence, optical...operating in continental shelf waters off Alaska’s north slope allowed us to construct proxy libraries for converting chlorophyll fluorescence to

Surface-tension driven open microfluidic platform for hanging droplet culture

PubMed Central

de Groot, T. E.; Veserat, K. S.; Berthier, E.; Beebe, D. J.; Theberge, A. B.

2015-01-01

The hanging droplet technique for three-dimensional tissue culture has been used for decades in biology labs, with the core technology remaining relatively unchanged. Recently microscale approaches have expanded the capabilities of the hanging droplet method, making it more user-friendly. We present a spontaneously driven, open hanging droplet culture platform to address many limitations of current platforms. Our platform makes use of two interconnected hanging droplet wells, a larger well where cells are cultured and a smaller well for user interface via a pipette. The two-well system results in lower shear stress in the culture well during fluid exchange, enabling shear sensitive or non-adherent cells to be cultured in a droplet. The ability to perform fluid exchanges in-droplet enables long-term culture, treatment, and characterization without disruption of the culture. The open well format of the platform was utilized to perform time-dependent coculture, enabling culture configurations with bone tissue scaffolds and cells grown in suspension. The open nature of the system allowed the direct addition or removal of tissue over the course of an experiment, manipulations that would be impractical in other microfluidic or hanging droplet culture platforms. PMID:26660268

Craniocervical injuries in judicial hangings: an anthropologic analysis of six cases.

PubMed

Spence, M W; Shkrum, M J; Ariss, A; Regan, J

1999-12-01

Restoration projects and archaeologic excavations in two Canadian prisons resulted in the recovery of the skeletons of six felons executed by judicial hanging. Damage inflicted by hanging on various skeletal elements was observed. Among the injuries seen were fractures of the hyoid cornua, styloid processes, occipital bones, and cervical vertebral bodies (C2) and transverse processes (C1, C2, C3, and C5). Despite the general uniformity of the hanging technique, which involved a subaural knot, the trauma to the skeletal elements and the cause of death varied among individuals. Although some of this variation was probably due to minor differences in hanging practices, individual anatomic peculiarities of the victims likely also contributed.

Educate a Hang Glider Pilot, a Backpacker.

ERIC Educational Resources Information Center

Kaufmann, Robert B.

1983-01-01

Outdoor education should educate people in, about, and for the out-of-doors, regardless of age or activity setting, and must document the effects technology has had on leisure as well as on outdoor education. (Author/MH)

Heelys and street gliders injuries: a new type of pediatric injury.

PubMed

Vioreanu, Mihai; Sheehan, Eoin; Glynn, Aaron; Casidy, Noelle; Stephens, Michael; McCormack, Damian

2007-06-01

Our goals were to highlight an increasing trend in orthopedic injuries in children as a result of "heeling" or "street gliding," to describe injuries sustained by children using Heelys (HSL, Carrollton, TX) and Street Gliders (Glowgadgets Ltd, Bristol, United Kingdom), and to increase public awareness and prevent such injuries. We prospectively recorded the data of all roller shoes injuries referred to our department during the summer school holiday. Using a data-collection sheet, we recorded demographic data, type of injury, mechanism and place of injury, heeling or street-gliding experience, use of safety equipment, methods of treatment, and intention to continue heeling or street gliding after recovery from injury. Over a 10-week period, 67 children suffered orthopedic injuries while using Heelys or Street Gliders. There were 56 girls and 11 boys with a mean age of 9.6 years. Upper limbs were the most common location of injury. Distal radius fractures were the most prevalent, followed by supracondylar fractures, elbow dislocations, and hand fractures. The majority of children suffered the injury while heeling or street gliding outdoors. Interestingly, 20% of the injuries happened while trying Heelys or Street Gliders for the first time, and 36% of the injuries occurred while learning (using 1-5 times) how to use them. None of the children used any sort of protective gear at the time of the injury. The majority of the injured children expressed their intention to continue heeling or street gliding after complete recovery from their injury. Our study shows that the majority of children with injuries from heeling or street gliding are girls. We recommend close supervision of children using Heelys or Street Gliders during the steep learning curve and usage of protective gear at all times. These new types of injuries have a serious impact on child health and constitute a burden for the pediatric orthopedic service.

Parametric geometric model and hydrodynamic shape optimization of a flying-wing structure underwater glider

NASA Astrophysics Data System (ADS)

Wang, Zhen-yu; Yu, Jian-cheng; Zhang, Ai-qun; Wang, Ya-xing; Zhao, Wen-tao

2017-12-01

Combining high precision numerical analysis methods with optimization algorithms to make a systematic exploration of a design space has become an important topic in the modern design methods. During the design process of an underwater glider's flying-wing structure, a surrogate model is introduced to decrease the computation time for a high precision analysis. By these means, the contradiction between precision and efficiency is solved effectively. Based on the parametric geometry modeling, mesh generation and computational fluid dynamics analysis, a surrogate model is constructed by adopting the design of experiment (DOE) theory to solve the multi-objects design optimization problem of the underwater glider. The procedure of a surrogate model construction is presented, and the Gaussian kernel function is specifically discussed. The Particle Swarm Optimization (PSO) algorithm is applied to hydrodynamic design optimization. The hydrodynamic performance of the optimized flying-wing structure underwater glider increases by 9.1%.

Properties of the Agulhas Current's Inshore Front During The Shelf Agulhas Glider Experiment (SAGE)

NASA Astrophysics Data System (ADS)

Krug, M.; Swart, S.; Goschen, W.

2016-02-01

The response of coastal and shelf regions to changes in the Agulhas Current remains poorly studied. This is partly due to observational challenges associated with sampling western boundary currents. Cross-shelf exchange in such energetic current systems occurs through a range of meso- ( 50-200 km) and sub-meso (<10 km) scale processes which are difficult to observe using moored current arrays or Lagrangian platforms. Profiling gliders offer a revolutionary technology to continuously sample the energetic inshore regions of the Agulhas Current at a high spatial (100's of meters to 3km - well within the sub-mesoscale range) and temporal (0.5-4 hourly) resolution. In April 2015, two SeaGliders were deployed off Port Elizabeth (34S) at the inshore edge of the Agulhas Current as part of the Shelf Agulhas Glider Experiment (SAGE), testing for the very 1st time the feasibility of operating autonomous platforms in this highly turbulent and energetic western boundary current system. For a period of approximately two months, the Seagliders provided continuous observations at the inshore boundary of the Agulhas Current at an unprecedented spatial resolution. Observations from the Seagliders showed that at the inshore edge of the Agulhas Current, both surface and depth averaged currents are aligned in a south-west / north- east direction, with stronger flows encountered over deeper regions of the shelf, when the gliders are closer to the Agulhas Current. In the absence of large meanders, the mean flow at the inshore boundary of the Agulhas Current is characterised by strong shear with a counter current flowing in opposite direction to the mean current field. Instances of counter currents occur 45% of the time in the surface flow and 54% of the time in the depth-averaged record. More than 80% of return flow occurrences occur when glider is in water depth of less than 200m.

Glider Surveys of Philippine Archipelago Seas

DTIC Science & Technology

2011-08-03

opening of the system of’ deeper straits connecting the SCS and Sulu Sea. The Sulu glider also completed a track across the Panay Gulf’ mouth -between...Observe diurnal isopycnal displacements and strain. • Examine the spatial structure of the sea’s response to Mindoro’s wind jets. • Contribute...than 1000 m. mean and -variable structure near a large reef, a sea’s response to wind jets and wakes, across-strait structure at the archipelago’s

Marginal Ice Zone: Biogeochemical Sampling with Gliders

DTIC Science & Technology

2013-09-30

melt and phytoplankton optical properties under Arctic ice. The project specific goals are to build collaboration with Arctic biogeochemists at...the ship to the larger spatial scales sampled by the gliders, and to estimate the contribution of phytoplankton to heating in the water column...Seagliders with from shipboard measurements taken on the R/V Araon and develop optical proxies for phytoplankton concentration, pigment spectral absorption

Development of An Autonomous Underwater Glider for Observing Physical Ocean Parameters in Indonesian Seas

NASA Astrophysics Data System (ADS)

Ajie Linarka, Utoyo; Riyanto Trilaksono, Bambang; Sagala, M. Faisal; Hidayat, Egi; Sopaheluwakan, Ardhasena; Rizal, Jose; Heriyanto, Eko; Amsal Harapan, Ferdika; Eka Syahputra Makmur, Erwin

2017-04-01

Conducting a sustained monitoring and surveying of physical ocean parameters for research or operational purposes using moorings and ships would require high cost. Development of an inexpensive instrument capable to perform such tasks not only could reduce cost and risks but also increase cruising range and depth. For that reason, a prototype of underwater glider was developed, named "GaneshBlue". GaneshBlue works based on gliding principles which utilizes pitch angle and buoyancy control for moving. For one gliding movement, GaneshBlue passed through 5 phases of surface, descent, transition, ascent and back to surface. The glider is equipped with basic navigation system and remote control, programmable survey planning, temperature and salinity sampling instruments, lithium batteries for power supply, and information processing software. A field test at the shallow water showed that GaneshBule has successfully demonstrated gliding and surfacing movements with surge motion speed reaching 20 cm s-1and 20 m in depths. During the field test the glider was also equipped with three instruments, i.e. Inertial Measurement Unit (IMU) to estimate glider's speed and orientation; MiniCT to acquire temperature and conductivity data; and Altisounder to determine its distance to sea surface and to seabed. In general, all the instruments performed well but filter algorithm needs to be implemented on data collection procedure to remove data outliers.

Retrospective analysis of 319 hanging and strangulation cases between 2001 and 2014 in Shanghai.

PubMed

Ma, Jianlong; Jing, Haojia; Zeng, Yan; Tao, Li; Yang, Yulei; Ma, Kaijun; Chen, Long

2016-08-01

In this study, we retrospectively analyzed 141 cases of hanging and 178 cases of ligature strangulation recorded in the Shanghai Municipal Public Security Bureau between January 2001 and December 2014 to explore the characteristics of hanging and ligature strangulation and to supply a scientific reference for forensic pathology. Several significant differences between hanging and ligature strangulation were found. Hanging cases were mostly suicide, with some accidental cases. Strangulation cases were mostly homicide, with a few cases of suicide or sexual asphyxia. Male hanging was more common than female hanging, with a ratio of 5:2. However, there were more female than male strangulation cases, with a ratio of 13:5. The ligature marks in hanging cases were almost all of a "U" type and above the hyoid bone. The ligature marks in strangulation cases were almost always a closed circle, but the position varied. The most common vital reactions were subcutaneous hemorrhage, exfoliation and blister, which are strong evidence of antemortem injury. Hemorrhagic spots were found on the temporalis, scalp, chest and back in strangulation cases, but were rare in hanging cases. Hemorrhagic manifestations were most common in the sternocleidomastoid muscle in hanging cases, and in the sternohyoid and sternothyroid muscles in strangulation cases. Fractures occurred in only ∼17% of victims. There are notable differences between hanging and ligature strangulation, which can help distinguish between these causes of death. These characteristics should be considered in forensic practice. Copyright © 2016 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

Return glider radiosonde to measure temperature, humidity and radiation profiles through the atmosphere

NASA Astrophysics Data System (ADS)

Kraeuchi, Andreas; Philipona, Rolf

2015-04-01

Very promising radiation profile measurements through the atmosphere were made in 2011 with a balloon borne short- and longwave net radiometer. New and improved radiation sensors from Kipp&Zonen are now used in a glider aircraft together with a standard Swiss radiosonde from Meteolabor AG. This new return glider radiosonde (RG-R), is lifted up with double balloon technique to prevent pendulum motion and to keep the radiation instruments as horizontal as possible during the ascent measuring phase. The RG-R is equipped with a mechanism that allows to release the radiosonde at a preset altitude, and an autopilot allowing to fly the radiosonde back to the launch site and to land it savely with a parachute at a preset location. The return glider radiosonde technique as well as new measurement possibilities will be shown. First measurements show temperature, humidity and radiation profiles through the atmosphere up to 30 hPa (24 km) during different atmospheric conditions. Radiation profiles during different daytimes show possibilities with respect to temporal resolution of vertical radiation profiles trough the atmosphere.

Energy consumption of ProTaper Next X1 after glide path with PathFiles and ProGlider.

PubMed

Berutti, Elio; Alovisi, Mario; Pastorelli, Michele Angelo; Chiandussi, Giorgio; Scotti, Nicola; Pasqualini, Damiano

2014-12-01

Instrument failure caused by excessive torsional stress can be controlled by creating a manual or mechanical glide path. The ProGlider single-file system (Dentsply Maillefer, Ballaigues, Switzerland) was recently introduced to perform a mechanical glide path. This study was designed to compare the effect of a glide path performed with PathFiles (Dentsply Maillefer) and ProGlider on torque, time, and pecking motion required for ProTaper Next X1 (Dentsply Maillefer) to reach the full working length in simulated root canals. Forty Endo Training Blocks (Dentsply Maillefer) were used. Twenty were prepared with a mechanical glide path using PathFiles 1 and 2 (the PathFile group), and 20 were prepared with a mechanical glide path using a ProGlider single file (the ProGlider group). All samples were shaped with ProTaper Next X1 driven by an endodontic motor connected to a digital wattmeter. The required torque for root canal instrumentation was analyzed by evaluating the electrical power consumption of the endodontic engine. Electric power consumption (mW/h), elapsed time (seconds), and number of pecking motions required to reach the full working length with ProTaper Next X1 were calculated. Differences among groups were analyzed with the parametric Student t test for independent data (P < .05). Elapsed time and electric power consumption were significantly different between groups (P = .0001 for both). ProGlider appears to perform more efficiently than PathFiles in decreasing electric power consumption of ProTaper Next X1 to reach the full working length. This study confirmed the ability of ProGlider to reduce stress in ProTaper Next X1 during shaping through a glide path and preliminary middle and coronal preflaring. Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Glider Pilot Written Test Guide: Private and Commercial.

ERIC Educational Resources Information Center

Federal Aviation Administration (DOT), Washington, DC. Flight Standards Service.

The intent of this guide is to define the scope and narrow the field of study as far as possible to the aeronautical knowledge required for qualifying for the private or commercial pilot (glider) certificate. Briefly summarized are type of test items used, hints for taking the test, and certificate requirements. The study outline is the basic…

Hydrographical and dynamical reconstruction of the Warm Core Cyprus Eddy from gliders data

NASA Astrophysics Data System (ADS)

Bosse, Anthony; Testor, Pierre; Hayes, Dan; Ruiz, Simon; Mauri, Elena; Charantonis, Anastase; d'Ortenzio, Fabrizio; Mortier, Laurent

2016-04-01

In the 80s, the POEM (Physical Oceanography of the Eastern Mediterranean) cruises in the Levantine Basin first revealed the presence of a very pronounced dynamical structure off Cyprus: The Cyprus Warm Core Eddy. Since then, a large amount of data have been collected thanks to the use of autonomous oceanic gliders (+8000 profiles since 2009). Part of those profiles were carried out in the upper layers down to 200 m, and we take benefit of a novel approach named ITCOMP SOM that uses a statistical approach to extend them down to 1000 m (see [1] for more details). This dataset have a particularly good spatio-temporal coverage in 2009 for about a month, thanks to simultaneous deployments of several gliders (up to 6). In this study, we present a set of 3D reconstruction of the dynamical and hydrographical characteristics of the Warm Core Cyprus Eddy between 2009 and 2015. Moreover, chlorophyll-a fluorescence data measured by the gliders give evidence to strong vertical velocities at the edge of the eddy. We discuss possible mechanisms (frontogenesis, symmetric instability) that could generate such signals and provide an assessment of the role of this peculiar circulation feature on the circulation and biogeochemistry of the Levantine basin. Reference: [1] Charantonis, A., P. Testor, L. Mortier, F. D'Ortenzio, S. Thiria (2015): Completion of a sparse GLIDER database using multi-iterative Self-Organizing Maps (ITCOMP SOM), Procedia Computer Science, 51(1):2198-2206. DOI: 10.1016/j.procs.2015.05.496

Coastal and mesoscale dynamics characterization combining glider and altimetry: case study over the Western Mediterranean Sea

NASA Astrophysics Data System (ADS)

Jerome, Bouffard; Pascual, Ananda; Ruiz, Simon; Isabelle Pujol, Marie; Faugere, Yannice; Larnicol, Gilles; Tintore, Joaquin

Satellite altimetry allows a direct computation of geostrophic velocity anomalies. However, conventional altimetry measurements remain largely spurious in coastal zone, due to several factors such as inaccurate geophysical corrections (e.g. atmospheric and tidal signals) as well as environmental issues (land contamination in the altimetric and radiometric footprints). At the present time and in the attempt of future relevant technologies (cf. SWOT satellite), experimen-tal coastal altimeter products are under development (XTRACK, PISTACH, COASTALT. . . ). The main efforts consist in the application of coastal-oriented corrections and the review of the data recovery strategies near the coast. The new coastal altimetric products need to be assessed with independent data before to be used in synergy with other measurements and fully exploited for scientific applications. This is the frame of this study as part of an intensive observational program conducted in the Western Mediterranean Sea. We present here the main outcomes resulting from the combination of coastal altimetry and gliders. Gliders -autonomous underwater vehicles -allow to provide precise and high resolution data complementary to altimetry (temperature, salinity, pressure, velocity. . . ) both at surface and over the whole water column. Since July 2007, several glider missions have been performed along Jason-1, Jason-2 and ENVISAT altimeters. The altimetric sea level anomalies have been processed from both standard and coastal-oriented strategies. Furthermore, new methodologies have also been developed in order to combine surface glider geostrophic velocities (derived from CTD measurements) with integrated currents estimated by the glider (derived from GPS locations every 6 hours). These approaches prove to be very efficient to improve the budget errors and homogenize the physical contents of altimetry and glider data. Further, the combined analysis of the two datasets provides interesting insights of

Vertical suspsended sediment fluxes observed from ocean gliders

NASA Astrophysics Data System (ADS)

Merckelbach, Lucas; Carpenter, Jeffrey

2016-04-01

Many studies trying to understand a coastal system in terms of sediment transport paths resort to numerical modelling - combining circulation models with sediment transport models. Two aspects herein are crucial: sediment fluxes across the sea bed-water column interface, and the subsequent vertical mixing by turbulence. Both aspects are highly complex and have relatively short time scales, so that the processes involved are implemented in numerical models as parameterisations. Due to the effort required to obtain field observations of suspended sediment concentrations (and other parameters), measurements are scarce, which makes the development and tuning of parameterisations a difficult task. Ocean gliders (autonomous underwater vehicles propelled by a buoyancy engine) provide a platform complementing more traditional methods of sampling. In this work we present observations of suspended sediment concentration (SSC) and dissipation rate taken by two gliders, each equipped with optical sensors and a microstructure sensor, along with current observations from a bottom mounted ADCP, all operated in the German Bight sector of the North Sea in Summer 2014. For about two weeks of a four-week experiment, the gliders were programmed to fly in a novel way as Lagrangian profilers to water depths of about 40 m. The benefit of this approach is that the rate of change of SSC - and other parameters - is local to the water column, as opposed to an unknown composition of temporal and spatial variability when gliders are operated in the usual way. Therefore, vertical sediment fluxes can be calculated without the need of the - often dubious - assumption that spatial variability can be neglected. During the experiment the water column was initially thermally stratified, with a cross-pycnocline diffusion coefficient estimated at 7\\cdot10-5 m2 s-1. Halfway through the experiment the remnants of tropical storm Bertha arrived at the study site and caused a complete mixing of the water

The influence of gravity on the distribution of the deposit formed onto a substrate by sessile, hanging, and sandwiched hanging drop evaporation.

PubMed

Sandu, Ion; Fleaca, Claudiu Teodor

2011-06-15

The focus of the present article is the study of the influence of gravity on the particle deposition profiles on a solid substrate during the evaporation of sessile, hanging and sandwiched hanging drops of colloidal particle suspensions. For concentrations of nanoparticles in the colloidal solutions in the range 0.0001-1 wt.%, highly diluted suspensions will preferentially form rings while concentrated suspensions will preferentially form spots in both sessile and hanging drop evaporation. For intermediary concentrations, the particle deposition profiles will depend on the nanoparticle aggregation dynamics in the suspension during the evaporation process, gravity and on the detailed evaporation geometry. The evaporation of a drop of toluene/carbon nanoparticle suspension hanging from a pendant water drop will leave on the substrate a circular spot with no visible external ring. By contrast, a clear external ring is formed on the substrate by the sessile evaporation of a similar drop of suspension sandwiched between a water drop and the substrate. From the application viewpoint, these processes can be used to create preferential electrical conductive carbon networks and contacts for arrays of self-assembled nanostructures fabricated on solid substrates as well as on flexible polymeric substrates. Copyright © 2011 Elsevier Inc. All rights reserved.

Study of endolaryngeal structures by videolaryngoscopy after hanging: a new approach to understanding the physiopathogenesis.

PubMed

Duband, S; Timoshenko, A P; Mohammedi, R; Prades, J-M; Barral, F-G; Debout, M; Péoc'h, M

2009-11-20

To evaluate laryngoscopic findings in hanging cases and to compare them with magnetic resonance imaging (MRI) and forensic autopsy results. Postmortem nasolaryngofibroscopy and MRI of five people who died from hanging were performed. Three people who died from other causes than hanging were also examined with a flexible laryngofibroscope. The results were compared with injuries discovered during forensic autopsy. In all five hanging cases, laryngofibroscopic investigation showed a vocal fold position in complete adduction confirmed by MRI. This position did not seem to be influenced by the intensity of the forces applied to neck or postmortem delay and cadaveric phenomena. The vocal cords of the three non-hanging deceased were found in the intermediate position. These findings could suggest that pressure applied to the cervical nervous and cartilaginous structures or their elongation during hanging could lead to closure of the glottis with vocal cord adduction maintained after death. Laryngofibroscopic examination in hanging cases could be very useful in confirming the vital character of the hanging and understanding asphyxial phenomena in incomplete suspension without laryngeal crush.

Long Term Autonomous Ocean Remote Sensing Utilizing the Wave Glider

NASA Astrophysics Data System (ADS)

Griffith, J.

2012-12-01

Rising costs of ship time and increasing budgetary restrictions make installation and maintenance of fixed ocean buoys a logistical and financial challenge. The cost associated with launch, recovery, and maintenance has resulted in a limited number of deployed buoys, restricting data on oceanic conditions. To address these challenges, Liquid Robotics (LRI) has developed the Wave Glider, an autonomous, mobile remote sensing solution. This system utilizes wave energy for propulsion allowing for long duration deployments of up to one year while providing real-time data on meteorological and oceanographic conditions. In November 2011, LRI deployed four Wave Gliders on a mission to cross the Pacific Ocean (the PacX) from San Francisco to Australia (two vehicles) or Japan (two vehicles) while transmitting data on weather conditions, wave profiles, sea surface temperatures, and biological conditions in real-time. This report evaluates the vehicle's ability to operate as an ocean going data platform by comparing data from the onboard weather sensors with two moored buoys, NDBC 46092 (Monterey Bay) and NDBC 51000 (200 nmi NE of Maui). The report also analyzes data transmitted from all four vehicles as they passed directly through a tropical storm 580 nmi NE of Hawaii. Upon arriving at one of the aforementioned buoys, the gliders continuously circled for a period of two days at a distance of three to eight nautical miles to build a comparative dataset. Data from both platforms were streamed in near real time enabling mid-mission evaluation of the performance of sensors. Overall, results varied from a <0.5% difference in barometric pressure between buoy NDBC 46092 and the gliders to high disagreement in wind speed and direction. While comparisons to moored buoy data can provide valuable insight into the relative accuracy of each platform, differences in agreement on variables such as wind speed and direction were attributed to micro-spatial variability in oceanic conditions

Challenges of implementing intervention research in persons with dementia: example of a glider swing intervention.

PubMed

Snyder, M; Tseng, Y H; Brandt, C; Croghan, C; Hanson, S; Constantine, R; Kirby, L

2001-01-01

Intervention studies for persons with dementia present many challenges. This article describes challenges encountered in conducting a glider swing intervention study for persons with dementia and the strategies used to manage the problems encountered. The overall purpose of a quasi-experimental study was to explore the effects of a glider swing intervention on emotions, relaxation, and aggressive behaviors in nursing home residents with dementia. Suggestions for investigators conducting intervention studies for persons with dementia are provided.

Subtidal Dynamics of the Vietnamese Shelf: Ship- and Glider-Based Observations of Hydrographic Structure and Circulation

DTIC Science & Technology

2013-09-30

glider endurance line off Vietnam, repeating NAGA sections that have not been occupied since the early 1960s, operated by Vietnamese scientists...1959-1961 NAGA expedition, using AUV gliders. We propose train the Vietnamese scientists by inviting and supporting them to visit OSU for a 1-2...biogeochemistry of the Vietnamese East Sea, including changes since the NAGA Expeditions In addition to planning, we have also been successful in bringing

Hanging Out with Which Friends? Friendship-Level Predictors of Unstructured and Unsupervised Socializing in Adolescence

PubMed Central

Siennick, Sonja E.; Osgood, D. Wayne

2012-01-01

Companions are central to explanations of the risky nature of unstructured and unsupervised socializing, yet we know little about whom adolescents are with when hanging out. We examine predictors of how often friendship dyads hang out via multilevel analyses of longitudinal friendship-level data on over 5,000 middle schoolers. Adolescents hang out most with their most available friends and their most generally similar friends, not with their most at-risk or similarly at-risk friends. These findings vary little by gender and wave. Together, the findings suggest that the risks of hanging out stem from the nature of hanging out as an activity, not the nature of adolescents’ companions, and that hanging out is a context for friends’ mutual reinforcement of pre-existing characteristics. PMID:23204811

Hanging Out with Which Friends? Friendship-Level Predictors of Unstructured and Unsupervised Socializing in Adolescence.

PubMed

Siennick, Sonja E; Osgood, D Wayne

2012-12-01

Companions are central to explanations of the risky nature of unstructured and unsupervised socializing, yet we know little about whom adolescents are with when hanging out. We examine predictors of how often friendship dyads hang out via multilevel analyses of longitudinal friendship-level data on over 5,000 middle schoolers. Adolescents hang out most with their most available friends and their most generally similar friends, not with their most at-risk or similarly at-risk friends. These findings vary little by gender and wave. Together, the findings suggest that the risks of hanging out stem from the nature of hanging out as an activity, not the nature of adolescents' companions, and that hanging out is a context for friends' mutual reinforcement of pre-existing characteristics.

Radiation Budget Profiles measured through the Atmosphere with a Return Glider Radiosonde

NASA Astrophysics Data System (ADS)

Philipona, R.; Kraeuchi, A.; Kivi, R.

2015-12-01

Very promising radiation budget profile measurements through the atmosphere were made in 2011 with a balloon borne short- and longwave net radiometer. New and improved radiation sensors from Kipp&Zonen are now used in a glider aircraft together with a standard Swiss radiosonde from Meteolabor AG. This new return glider radiosonde (RG-R), is lifted up with double balloon technique to prevent pendulum motion and to keep the radiation instruments as horizontal as possible during the ascent measuring phase. The RG-R is equipped with a release mechanism and an autopilot that flies the glider radiosonde back to the launch site, or to a predefined open space, where it releases a parachute for landing once it is 100 meter above ground. The RG-R was successfully tested and deployed for tropospheric and stratospheric radiation measurements up to 30 hPa (24 km altitude) at the GRUAN sites Payerne (Switzerland) and Sodankylä (Finland). Radiation profiles and the radiation budget through the atmosphere during different daytimes and under cloud-free and cloudy situations will be shown in relation to temperature and humidity at the surface and in the atmosphere. The RG-R flight characteristics and new measurement possibilities will also be discussed.

The Relative Concentrations of Nutrients and Toxins Dictate Feeding by a Vertebrate Browser, the Greater Glider Petauroides volans.

PubMed

Jensen, Lora M; Wallis, Ian R; Foley, William J

2015-01-01

Although ecologists believe that vertebrate herbivores must select a diet that allows them to meet their nutritional requirements, while avoiding intoxication by plant secondary metabolites, this is remarkably difficult to show. A long series of field and laboratory experiments means that we have a good understanding of the factors that affect feeding by leaf-eating marsupials. This knowledge and the natural intraspecific variation in Eucalyptus chemistry allowed us to test the hypothesis that the feeding decisions of greater gliders (Petauroides volans) depend on the concentrations of available nitrogen (incorporating total nitrogen, dry matter digestibility and tannins) and of formylated phloroglucinol compounds (FPCs), potent antifeedants unique to Eucalyptus. We offered captive greater gliders foliage from two species of Eucalyptus, E. viminalis and E. melliodora, which vary naturally in their concentrations of available nitrogen and FPCs. We then measured the amount of foliage eaten by each glider and compared this with our laboratory analyses of foliar total nitrogen, available nitrogen and FPCs for each tree offered. The concentration of FPCs was the main factor that determined how much gliders ate of E. viminalis and E. melliodora, but in gliders fed E. viminalis the concentration of available nitrogen was also a significant influence. In other words, greater gliders ate E. viminalis leaves with a particular combination of FPCs and available nitrogen that maximised the nutritional gain but minimised their ingestion of toxins. In contrast, the concentration of total nitrogen was not correlated with feeding. This study is among the first to empirically show that browsing herbivores select a diet that balances the potential gain (available nutrients) and the potential costs (plant secondary chemicals) of eating leaves. The major implication of the study is that it is essential to identify the limiting nutrients and relevant toxins in a system in order to

The Relative Concentrations of Nutrients and Toxins Dictate Feeding by a Vertebrate Browser, the Greater Glider Petauroides volans

PubMed Central

Jensen, Lora M.; Wallis, Ian R.; Foley, William J.

2015-01-01

Although ecologists believe that vertebrate herbivores must select a diet that allows them to meet their nutritional requirements, while avoiding intoxication by plant secondary metabolites, this is remarkably difficult to show. A long series of field and laboratory experiments means that we have a good understanding of the factors that affect feeding by leaf-eating marsupials. This knowledge and the natural intraspecific variation in Eucalyptus chemistry allowed us to test the hypothesis that the feeding decisions of greater gliders (Petauroides volans) depend on the concentrations of available nitrogen (incorporating total nitrogen, dry matter digestibility and tannins) and of formylated phloroglucinol compounds (FPCs), potent antifeedants unique to Eucalyptus. We offered captive greater gliders foliage from two species of Eucalyptus, E. viminalis and E. melliodora, which vary naturally in their concentrations of available nitrogen and FPCs. We then measured the amount of foliage eaten by each glider and compared this with our laboratory analyses of foliar total nitrogen, available nitrogen and FPCs for each tree offered. The concentration of FPCs was the main factor that determined how much gliders ate of E. viminalis and E. melliodora, but in gliders fed E. viminalis the concentration of available nitrogen was also a significant influence. In other words, greater gliders ate E. viminalis leaves with a particular combination of FPCs and available nitrogen that maximised the nutritional gain but minimised their ingestion of toxins. In contrast, the concentration of total nitrogen was not correlated with feeding. This study is among the first to empirically show that browsing herbivores select a diet that balances the potential gain (available nutrients) and the potential costs (plant secondary chemicals) of eating leaves. The major implication of the study is that it is essential to identify the limiting nutrients and relevant toxins in a system in order to

The epidemiology of injury in hang-gliding and paragliding.

PubMed

Rekand, Tiina

2012-01-01

Para- and hang-gliding are modern air sports that developed in the 20th century. Performers should possess technical skills and manage certified equipment for successful flight. Injuries may happen during the take-off, flight and landing. PubMed was searched using the search terms 'paragliding' and/or 'hang-gliding'. The reference lists of articles identified in the search strategy were also searched for relevant articles. The most common injuries are fractures, dislocations or sprains in the extremities, followed by spinal and head traumas. Multiple injuries after accidents are common. Collision with electrical wires may cause burn injuries. Fatal outcomes are caused by brain injuries, spinal cord injuries at the cervical level or aorta rupture. Accidents happen because of risk-taking behavior, lack of education or use of self-modified equipment. Observational studies have suggested the need for protection of the head, trunk and lower extremities. The measures proposed are often based on conclusions of observational studies and not proven through randomized studies. Better education along with focusing on possible risk factors will probably diminish the risks of hang- and paragliding. Large denominator-based case series, case-control and population-based studies are needed for assessment of the risks of hang- and paragliding. Copyright © 2012 S. Karger AG, Basel.

Water collection behavior and hanging ability of bioinspired fiber.

PubMed

Hou, Yongping; Chen, Yuan; Xue, Yan; Zheng, Yongmei; Jiang, Lei

2012-03-13

Since the water-collecting ability of the wetted cribellate spider capture silk is the result of a unique fiber structure, bioinspired fibers have been researched significantly so as to expose a new water-acquiring route in fogging-collection projects. However, the design of the geometry of bioinspired fiber is related to the ability of hanging drops, which has not been investigated in depth so far. Here, we fabricate bioinspired fibers to investigate the water collection behavior and the influence of geometry (i.e., periodicity of spindle knot) on the hanging-drop ability. We especially discuss water collection related to the periodicity of geometry on the bioinspired fiber. We reveal the length of the three phase contact line (TCL) at threshold conditions in conjunction with the maximal volume of a hanging drop at different modes. The study demonstrates that the geometrical structure of bioinspired fiber induces much stronger water hanging ability than that of uniform fiber, attributed to such special geometry that offers effectively an increasing TCL length or limits the contact length to be shorted. In addition, the geometry also improves the fog-collection efficiency by controlling tiny water drops to be collected in the large water drops at a given location.

Looking for an accident: glider pilots' visual management and potentially dangerous final turns.

PubMed

Jarvis, Steve; Harris, Don

2007-06-01

Accidents caused by spinning from low turns continue to kill glider pilots despite the introduction of specific exercises aimed at increasing pilot awareness and recognition of this issue. In-cockpit video cameras were used to analyze flying accuracy and log the areas of visual interest of 36 qualified glider pilots performing final turns in a training glider. Pilots were found to divide their attention between four areas of interest: the view directly ahead; the landing area (right); the airspeed indicator; and an area between the direct ahead view and the landing area. The mean fixation rate was 85 shifts per minute. Significant correlations were found between over-use of rudder and a lack of attention to the view ahead, as well as between the overall fixation rate and poorer coordination in the turn. The results provide some evidence that a relationship exists between pilots' visual management and making turns in a potentially dangerous manner. Pilots who monitor the view ahead for reasonable periods during the final turn while not allowing their scan to become over-busy are those who are most likely to prevent a potential spin.

Mapping the sound field of an erupting submarine volcano using an acoustic glider.

PubMed

Matsumoto, Haru; Haxel, Joseph H; Dziak, Robert P; Bohnenstiehl, Delwayne R; Embley, Robert W

2011-03-01

An underwater glider with an acoustic data logger flew toward a recently discovered erupting submarine volcano in the northern Lau basin. With the volcano providing a wide-band sound source, recordings from the two-day survey produced a two-dimensional sound level map spanning 1 km (depth) × 40 km(distance). The observed sound field shows depth- and range-dependence, with the first-order spatial pattern being consistent with the predictions of a range-dependent propagation model. The results allow constraining the acoustic source level of the volcanic activity and suggest that the glider provides an effective platform for monitoring natural and anthropogenic ocean sounds. © 2011 Acoustical Society of America

Harvesting the High-Hanging Fruit

ERIC Educational Resources Information Center

Kenton, Jay D.

2014-01-01

For many years, higher education institutions have been harvesting the low-hanging fruit when it comes to budget reductions and adjustments. Easier changes have often been made--such as cutting administration, using more adjunct faculty, contracting out inefficient or non effective auxiliary operations and so forth. Until recently such strategies,…

36 CFR § 327.4 - Aircraft.

Code of Federal Regulations, 2013 CFR

2013-07-01

... REGULATIONS GOVERNING PUBLIC USE OF WATER RESOURCE DEVELOPMENT PROJECTS ADMINISTERED BY THE CHIEF OF ENGINEERS..., seaplanes, helicopters, ultra-light aircraft, motorized hang gliders, hot air balloons, any non-powered... person, material or equipment by parachute, balloon, helicopter or other means onto or from project lands...

Ocean gliders as key component within the AORAC-SA

NASA Astrophysics Data System (ADS)

Barrera, C.; Hernandez Brito, J.; Castro, A.; Rueda, M. J.; Llinas, O.

2016-02-01

The Atlantic Ocean Research Alliance Coordination and Support Action (AORAC-SA) is designed to provide scientific, technical and logistical support to the EU in developing and implementing transAtlantic Marine Research Cooperation between the European Union, the United States of America and Canada. The Coordination and Support Action (CSA) is carried out within the framework of the Atlantic Ocean Research Alliance (AORA) as outlined in the Galway Statement on Atlantic Ocean Cooperation (May 2013). The CSA will be responsible for the organization of expert and stakeholder meetings, workshops and conferences required by the AORA and related to identified research priorities support actions and other initiatives as they arise, taking into account related Horizon 2020 supported transAtlantic projects and on-going national and EU collaborative projects. The AORAC-SA support and governance structure comprises a Secretariat and Management Team, guided by a high-level Operational Board, representative of the major European Marine Research Programming and Funding Organizations as well as those of the USA and Canada. As example of this research cooperative framework, ocean gliders have become nowadays a common, innovative and sustainable ocean-observations tool for the Atlantic basin, linking research groups, govermental institutions and private companies from both sides in terms of technical developments, transatlantic missions in partnership, training forums, etc. aiming to develop common practices and protocols for a better ocean resources management and understanding. Within this context, the Oceanic Platform of the Canary Islands (PLOCAN), as AORAC-SA partner, is working on specific actions like ocean glider observations programs (endurance line) by AtlantOS project (www.atlantos-h2020.eu), related new technical developments by NeXOS FP-7 project (www.nexosproject.eu) and a yearly International Glider School forum hosting (www.gliderschool.eu).

First report of parasitism by Ophidascaris robertsi (Nematoda) in a sugar glider (Petaurus breviceps, Marsupialia).

PubMed

Gallego Agúndez, Miguel; Villaluenga Rodríguez, Jose Enrique; Juan-Sallés, Carles; Spratt, David M

2014-12-01

Third-stage larvae of Ophidascarsis robertsi (Nematoda: Ascaridoidea) were found on necropsy in a female sugar glider, Petaurus breviceps (Marsupialia: Petauridae), two in heart chambers and one free in the peritoneal cavity. The animal was bred in captivity and had previous contact with Australian pythons captured in nature, which could be the source of the infection. The histopathologic diagnosis was intraluminal and perivascular pulmonary hemorrhage possibly due to the parasitosis. It is the first report of parasitism by O. robertsi in a sugar glider.

GPS-Acoustic Seafloor Geodesy using a Wave Glider

NASA Astrophysics Data System (ADS)

Chadwell, C. D.

2013-12-01

The conventional approach to implement the GPS-Acoustic technique uses a ship or buoy for the interface between GPS and Acoustics. The high cost and limited availability of ships restricts occupations to infrequent campaign-style measurements. A new approach to address this problem uses a remote controlled, wave-powered sea surface vehicle, the Wave Glider. The Wave Glider uses sea-surface wave action for forward propulsion with both upward and downward motions producing forward thrust. It uses solar energy for power with solar panels charging the onboard 660 W-h battery for near continuous operation. It uses Iridium for communication providing command and control from shore plus status and user data via the satellite link. Given both the sea-surface wave action and solar energy are renewable, the vehicle can operate for extended periods (months) remotely. The vehicle can be launched from a small boat and can travel at ~ 1 kt to locations offshore. We have adapted a Wave Glider for seafloor geodesy by adding a dual frequency GPS receiver embedded in an Inertial Navigation Unit, a second GPS antenna/receiver to align the INU, and a high precision acoustic ranging system. We will report results of initial testing of the system conducted at SIO. In 2014, the new approach will be used for seafloor geodetic measurements of plate motion in the Cascadia Subduction Zone. The project is for a three-year effort to measure plate motion at three sites along an East-West profile at latitude 44.6 N, offshore Newport Oregon. One site will be located on the incoming plate to measure the present day convergence between the Juan de Fuca and North American plates and two additional sites will be located on the continental slope of NA to measure the elastic deformation due to stick-slip behavior on the mega-thrust fault. These new seafloor data will constrain existing models of slip behavior that presently are poorly constrained by land geodetic data 100 km from the deformation front.

Mesoscale Circulation Variability from Five years of Quasi-continuous Glider Observations and Numerical Simulation at a Key Sub-basin 'Choke' Point.

NASA Astrophysics Data System (ADS)

Heslop, E. E.; Mourre, B.; Juza, M.; Troupin, C.; Escudier, R.; Torner, M.; Tintore, J.

2016-02-01

Quasi-continuous glider observations over 5 years have uniquely characterised a high frequency variability in the circulation through the Ibiza Channel, an important `choke' point in the Western Mediterranean Sea. This `choke' point governs the basin/sub-basin scale circulation and the north/south exchanges of different water masses. The resulting multi-scale variability impacts the regional shelf and open ocean ecosystems, including the spawning grounds of Atlantic bluefin tuna. Through the unique glider record we show the relevance of the weekly/mesoscale variability, which is of same order as the previously established seasonal and inter-annual variability. To understand the drivers of this variability we combine the glider data with numerical simulations (WMOP) and altimetry. Two key drivers are identified; extreme winter events, which cause the formation of a cold winter mode water (Winter Intermediate Water) in the shelf areas to the north of the Ibiza Channel, and mesoscale activity, which to the north produce channel `blocking' eddies and to the south intermittent and vigorous flows of fresher `Atlantic' waters through the Ibiza Channel. Results from the 2 km resolution WMOP are compared with the high-resolution (2 - 3 km.) glider data, giving insight into model validation across different scales, for both circulation and water masses. There is an emerging consensus that gliders can uniquely access critical time and length scales and in this study gliders complement existing satellite measurements and models, while opening up new capabilities for multidisciplinary, autonomous and high-resolution ocean observation.

77 FR 2234 - Airworthiness Directives; DG Flugzeugbau GmbH Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-17

... Airworthiness Directives; DG Flugzeugbau GmbH Gliders AGENCY: Federal Aviation Administration (FAA), Department... new airworthiness directive (AD) for DG Flugzeugbau GmbH Models DG-500 Elan Orion, DG-500 Elan Trainer... DG- Flugzeugbau GmbH, Otto-Lilienthal-Weg 2, 76646 Bruchsal, Federal Republic of Germany; telephone...

The Development, Design and Construction of Gliders and Sailplanes

NASA Technical Reports Server (NTRS)

Lippisch, A

1931-01-01

This report explains and details the history of glider design and construction through the impetus of the Rhon Sailplane contests. Some of the topics considered include: whether the longitudinal stability (dynamic and static) is sufficient in the tailless typo and a consideration that the structural weight of sweptback wings would be greater than that of a corresponding normal wing.

[Accidental hanging during auto-erotic practices].

PubMed

Vieira, D N; da Silva, A G

1989-01-01

An unusual case of accidental hanging during autoerotic practices in a 25-year-old male student is described and the autoerotic asphyxia syndrome briefly discussed. The authors stressed the importance of a correct diagnostic of accidental death in these cases.

The conundrum of hanging points in correctional facilities.

PubMed

Pridmore, Saxby; Pridmore, William

2017-02-01

We aimed to explore aspects of the removal of hanging points from correctional facilities. An argument can be made that individuals have a right to die/suicide. The United Nations holds that except for freedom of movement, prisoners have the same rights as non-prisoners. The rights of the individual and the duty of many custodial institutions are in conflict. This introduces a conundrum; when all hanging points are removed from correctional facilities, prisoners without mental disorder, but with a sustained wish to die, will not be able to achieve that end, while non-prisoners will be well able to do so.

Expedition 54 plaque hanging ceremony

NASA Image and Video Library

2018-04-26

NASA astronauts Joe Acaba and Mark Vande Hei Acaba visited Marshall April 11 for their honorary Expedition 54 plaque hanging ceremony and to provide valuable feedback of their on-orbit science investigations with the Payload Operations and Integration Center team. Astronauts Mark Vande Hei (L) and Astronaut Joe Acaba sign autographs for Kassandra Stephens

Expedition 54 plaque hanging ceremony

NASA Image and Video Library

2018-04-26

NASA astronauts Joe Acaba and Mark Vande Hei Acaba visited Marshall April 11 for their honorary Expedition 54 plaque hanging ceremony and to provide valuable feedback of their on-orbit science investigations with the Payload Operations and Integration Center team. Astronauts Mark Vande Hei (L) and Astronaut Joe Acaba sign autographs for Lori Meggs.

Expedition 54 plaque hanging ceremony

NASA Image and Video Library

2018-04-26

NASA astronauts Joe Acaba and Mark Vande Hei Acaba visited Marshall April 11 for their honorary Expedition 54 plaque hanging ceremony and to provide valuable feedback of their on-orbit science investigations with the Payload Operations and Integration Center team. Payload Operations Director Phillipia Simmons with Astronauts Joe Acaba (L) and Mark Vande Hei

Expedition 54 plaque hanging ceremony

NASA Image and Video Library

2018-04-26

NASA astronauts Joe Acaba and Mark Vande Hei Acaba visited Marshall April 11 for their honorary Expedition 54 plaque hanging ceremony and to provide valuable feedback of their on-orbit science investigations with the Payload Operations and Integration Center team. Astronauts Mark Vande Hei (L) and Astronaut Joe Acaba sign autographs for Samantha Gurley.

Motorless flight research, 1972. [glider design, airframes, and soaring flight developments

NASA Technical Reports Server (NTRS)

Nash-Webber, J. L. (Editor)

1973-01-01

The proceedings of a symposium on motorless flight research are presented. The subjects discussed are: (1) glider aerodynamic and design, (2) instrumentation, (3) structural concepts and materials, (4) soaring meteorology, (5) self-launching and ultralight sailplanes, and (6) performance testing.

GNSS Wave Glider: First results from Loch Ness and demonstration of its suitability for determining the marine geoid

NASA Astrophysics Data System (ADS)

Penna, N. T.; Morales Maqueda, M.; Williams, S. D.; Foden, P.; Martin, I.; Pugh, J.

2013-12-01

We report on a first deployment of a GNSS Wave Glider designed for precise, unmanned, autonomous, mobile self-propelled sea level and sea state measurement in the open ocean. The Wave Glider, equipped with a dual frequency GPS+GLONASS receiver, was deployed in Loch Ness, Scotland, autonomously travelling 32 km in a north-easterly direction along the length of the loch in 26 hours, propelled by energy generated from waves of typical amplitude only 100-150 mm and frequency on the order 0.5-1 Hz. The Wave Glider GNSS data were analysed using a post-processed kinematic GPS+GLONASS precise point positioning (PPP) approach, which were quality controlled using double difference GPS kinematic processing with respect to onshore reference stations at either end of the loch. The PPP heights of the loch's surface revealed a clear geoid gradient of about 30 mm/km (i.e. just under 1 m over the whole length of the loch), very similar to both the EGM2008 and OSGM02 geoid models, demonstrating the potential use of a GNSS Wave Glider for marine geoid determination. After applying a low pass filter, the GNSS heights showed local deviations from both EGM2008 and OSGM02, potentially caused by omission errors or a lack of gravity data over Loch Ness. In addition to dual frequency GNSS data, the Wave Glider also recorded inclinometer data, bathymetry, and surface currents, which, in combination with tide gauge and wind data, were used to further control and interpret the GNSS time series.

A long term glider study of shelf sea oxygen dynamics

NASA Astrophysics Data System (ADS)

Williams, Charlotte; Palmer, Matthew; Mahaffey, Claire; Davis, Clare

2017-04-01

Oxygen is involved in most biogeochemical processes in the ocean, and dissolved oxygen (DO) is a well-established indicator for biological activity via the estimate of apparent oxygen utilisation (AOU). In the deep waters of the open ocean, the AOU provides a valuable insight into the ocean's biological carbon pump. However, in the physically dynamic and highly productive shallow shelf seas, interpretation of the oxygen distribution and the magnitude of AOU is complex. Physical processes, such as diapycnal mixing, entrainment and horizontal advection act to ventilate waters below the thermocline and thus increase oxygen and decrease AOU. In contrast, biological remineralisation of organic material below the thermocline will consume oxygen and increase AOU. We aim to address the following: 1. Does AOU change seasonally in a shelf sea in response to seasonal changes in productivity? 2. How important is turbulence in redistributing oxygen in a shelf sea? Using 9 months of high-resolution data from >20 glider deployments in the seasonally stratified NW European Shelf Sea we identify and quantify the physical and biological processes that control the DO distribution and magnitude of AOU in shelf seas. A 200km transect between the shelf edge and the central Celtic Sea (CCS) was repeated between November 2014 and August 2015, thus capturing key periods in the seasonal cycling in shelf seas, specifically the onset of stratification, the spring bloom, stratified summer period and breakdown of stratification. The gliders collected data for DO, temperature, salinity, chlorophyll fluorescence, CDOM, backscatter and turbulence. In addition, direct measurements of turbulent dissipation from the Ocean Microstructure Glider deployed during the campaign provided estimates of mixing at CCS and the shelf break, allowing accurate quantification of the vertical fluxes of oxygen. We find that oxygen decreases ubiquitously across the shelf as soon as stratification takes hold, though BML

Accidental hanging by a T-shirt collar in a man with morphine intoxication: an unusual case.

PubMed

Kodikara, Sarathchandra; Alagiyawanna, Ramesh

2011-09-01

Accidental hanging is rare across all age groups, and it is even rarer in the adult population except in autoerotic asphyxia. Few cases have been reported in the literature, which describe unusual patterns of accidental hanging. This article focuses on an unusual pattern of accidental hanging of a 25-year-old man, who was in a state of morphine-induced central nervous system depression and found dead in a sitting position with the collar of his T-shirt hanging off a jutting-out root of a tree. The hanged collar acted as a ligature compressing the neck.

Distribution of hanging garden vegetation associations on the Colorado Plateau, USA

Treesearch

James F. Fowler; N. L. Stanton; Ronald L. Hartman

2007-01-01

Hanging gardens are island-like habitats dominated by mesophytic-hydrophytic plant communities, growing on seeps on the xeric canyon walls of the Colorado Plateau in the American West. We measured the abundance of species and physical microhabitat characteristics of 73 individual hanging gardens during the growing seasons of 1991-1993. Cluster analysis of a simplified...

Functional anatomy of gliding membrane muscles in the sugar glider (Petaurus breviceps).

PubMed

Endo, H; Yokokawa, K; Kurohmaru, M; Hayashi, Y

1998-02-01

In order to clarify the morphological adaptation for gliding behavior in the marsupial mammals, the gliding membrane muscles in the sugar glider (Petaurus breviceps) were observed. Unlike the styliform cartilage in flying squirrels, the sugar glider has a well-developed tibiocarpalis muscle in the most lateral area of the gliding membrane. The gliding membrane substantially consists of the humerodorsalis and tibioabdominalis muscle complex. We believe that the thick tibiocarpalis bundle and the humerodorsalis and tibioabdominalis muscle complex may serve as a membrane controller in the gliding behavior. A characteristic thin membranous structure between the cutaneous and deeper muscles was observed. In addition to the direct powerful control exerted by trunk and limb movement, we suggest that indirect power conduction by this thin membranous structure may contribute to gliding membrane control.

Interim Heavy Airlift: Sea Base Logistics Glider Concept

DTIC Science & Technology

2007-01-01

the thin air at such a high elevation decreased the glider’s takeoff performance , requiring a longer distance to match the tug’s speed . This increased...packaging, handling, shipping , and transport (PHST) of both the cargo payload and the new air vehicle. Logistics gliders require both a capable supply...bypasses these littoral and ashore links at a high speed of advance, making the concept effective and attractive. The issue then becomes defining

77 FR 19063 - Airworthiness Directives; DG Flugzeugbau GmbH Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-30

... Airworthiness Directives; DG Flugzeugbau GmbH Gliders AGENCY: Federal Aviation Administration (FAA), Department... (AD) for DG Flugzeugbau GmbH Models DG-500 Elan Orion, DG-500 Elan Trainer, DG-500/ 20 Elan, DG-500/22... identified in this AD, contact DG- Flugzeugbau GmbH, Otto-Lilienthal-Weg 2, 76646 Bruchsal, Federal Republic...

Calculation of the bending of electromechanical aircraft element made of the carbon fiber

NASA Astrophysics Data System (ADS)

Danilova-Volkovskaya, Galina; Chepurnenko, Anton; Begak, Aleksandr; Savchenko, Andrey

2017-10-01

We consider a method of calculation of an orthotropic plate with variable thickness. The solution is performed numerically by the finite element method. The calculation is made for the springs of a hang glider made of carbon fiber. The comparison of the results with Sofistik software complex is given.

Relationship Between the Sonic Layer Depth and Mixed Layer Depth Identified from U.S. Navy Sea Glider Data

DTIC Science & Technology

2014-09-01

LAYER DEPTH AND MIXED LAYER DEPTH IDENTIFIED FROM U.S. NAVY SEA GLIDER DATA Vance A. Villarreal September 2014 Thesis Advisor: Peter C. Chu Second...searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments...DEPTH IDENTIFIED FROM U.S. NAVY SEA GLIDER DATA 5. FUNDING NUMBERS 6. AUTHOR(S) Vance A. Villarreal 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES

Hanging out with Which Friends? Friendship-Level Predictors of Unstructured and Unsupervised Socializing in Adolescence

ERIC Educational Resources Information Center

Siennick, Sonja E.; Osgood, D. Wayne

2012-01-01

Companions are central to explanations of the risky nature of unstructured and unsupervised socializing, yet we know little about whom adolescents are with when hanging out. We examine predictors of how often friendship dyads hang out via multilevel analyses of longitudinal friendship-level data on over 5,000 middle schoolers. Adolescents hang out…

The 2014-2015 warming anomaly in the Southern California Current System observed by underwater gliders

NASA Astrophysics Data System (ADS)

Zaba, Katherine D.; Rudnick, Daniel L.

2016-02-01

Large-scale patterns of positive temperature anomalies persisted throughout the surface waters of the North Pacific Ocean during 2014-2015. In the Southern California Current System, measurements by our sustained network of underwater gliders reveal the coastal effects of the recent warming. Regional upper ocean temperature anomalies were greatest since the initiation of the glider network in 2006. Additional observed physical anomalies included a depressed thermocline, high stratification, and freshening; induced biological consequences included changes in the vertical distribution of chlorophyll fluorescence. Contemporaneous surface heat flux and wind strength perturbations suggest that local anomalous atmospheric forcing caused the unusual oceanic conditions.

Deadzones, Dying Eddies, and the Loop Current: Stability, Ventilation, and Heat Content from Buoyancy Glider Observations in the Northwest Gulf of Mexico in Spring and Summer 2015

NASA Astrophysics Data System (ADS)

DiMarco, S. F.; Knap, A. H.; Wang, Z.; Walpert, J.; Dreger, K.

2016-02-01

The northwestern Gulf of Mexico is host to a myriad of physical and biochemical processes, which govern the exchange and transport of material and volume between the coastal and offshore environments. We report on five G2 Slocum glider deployments in the northwestern Gulf during the spring and summer of 2015. The gliders were deployed in shallow (20 m) and deep (greater than 1000 m) water for a total of about 200 days. During this time, the gliders encountered a variety of environmental conditions that impact the circulation, biology, chemistry of the shelf and slope. The shallow gliders encountered coastal waters influenced by extensive flooding in terrestrial Texas that vertically stratified the water-column and was coincident with sub-pycnocline low dissolved oxygen concentration, at times below the hypoxic threshold of 2 mg/L, and elevated CDOM concentrations. These gliders also reveal high spatial variability with bottom boundary oxygen and biomass scales on the order of a few kilometers. The deep gliders were tasked to investigate shelf/slope exchange at two locations 94W and 91W. The western glider encountered a mature mesoscale circulation eddy that was actively weakening. The eastern glider simultaneously encountered a freshly separated Loop Current eddy. The vertical structure of hydrographic and dissolved oxygen parameters shows significant and distinguishable variability in each feature. The vertical structure of both features show significant departures from that which is expected based on sea surface height determined from satellite altimetry. Additionally, glider observations are compared to operational high-resolution regional numerical model output. These observations emphasize the importance of direct observations over satellite-derived products for applications that include upper ocean heat content for hurricane intensification and vertical mixing and ventilation of the oceanic interior.

EAST Multicenter Trial on Targeted Temperature Management for Hanging-Induced Cardiac Arrest.

PubMed

Hsu, Cindy H; Haac, Bryce E; Drake, Mack; Bernard, Andrew C; Aiolfi, Alberto; Inaba, Kenji; Hinson, Holly E; Agarwal, Chinar; Galante, Joseph; Tibbits, Emily M; Johnson, Nicholas J; Carlbom, David; Mirhoseini, Mina F; Patel, Mayur B; OʼBosky, Karen R; Chan, Christian; Udekwu, Pascal O; Farrell, Megan; Wild, Jeffrey L; Young, Katelyn A; Cullinane, Daniel C; Gojmerac, Deborah J; Weissman, Alexandra; Callaway, Clifton; Perman, Sarah M; Guerrero, Mariana; Aisiku, Imoigele P; Seethala, Raghu R; Co, Ivan N; Madhok, Debbie Y; Darger, Bryan; Kim, Dennis Y; Spence, Lara; Scalea, Thomas M; Stein, Deborah M

2018-04-19

We sought to determine the outcome of suicidal hanging and the impact of targeted temperature management (TTM) on hanging-induced cardiac arrest (CA) through an Eastern Association for the Surgery of Trauma (EAST) multicenter retrospective study. We analyzed hanging patient data and TTM variables from January 1992 to December 2015. Cerebral performance category (CPC) score of 1 or 2 was considered good neurologic outcome, while CPC of 3 or 4 was considered poor outcome. Classification and Regression Trees (CART) recursive partitioning was used to develop multivariate predictive models for survival and neurological outcome. Total of 692 hanging patients from 17 centers were analyzed for this study. Their overall survival rate was 77%, and the CA survival rate was 28.6%. The CA patients had significantly higher severity of illness and worse outcome than the non-CA patients. Of the 175 CA patients who survived to hospital admission, 81 patients (46.3%) received post-cardiac arrest TTM. The unadjusted survival of TTM CA patients (24.7% vs 39.4%, p<0.05) and good neurologic outcome (19.8% vs 37.2%, p<0.05) were worse than non-TTM CA patients. However, when subgroup analyses were performed between those with admission GCS of 3-8, the differences between TTM and non-TTM CA survival (23.8% vs 30.0%, p=0.37) and good neurologic outcome (18.8% vs 28.7%, p=0.14) were not significant. TTM implementation and post-cardiac arrest management varied between the participating centers. CART models identified variables predictive of favorable and poor outcome for hanging and TTM patients with excellent accuracy. CA hanging patients had worse outcome than non-CA patients. TTM CA patients had worse unadjusted survival and neurologic outcome than non-TTM patients. These findings may be explained by their higher severity of illness, variable TTM implementation, and differences in post-cardiac arrest management. Future prospective studies are necessary to ascertain the effect of TTM on

Modified liver hanging maneuver focusing on outflow control in pure laparoscopic left-sided hepatectomy.

PubMed

Kim, Ji Hoon

2018-04-01

Outflow control during laparoscopic liver resection necessitates the use of technically demanding procedures since the hepatic veins are fragile and vulnerable to damage during parenchymal transection. The liver hanging maneuver reduces venous backflow bleeding during deep parenchymal transection. The present report describes surgical outcomes and a technique to achieve outflow control during application of the modified liver hanging maneuver in patients undergoing laparoscopic left-sided hepatectomy. A retrospective review was performed of clinical data from 29 patients who underwent laparoscopic left-sided hepatectomy using the modified liver hanging maneuver between February 2013 and March 2017. For this hanging technique, the upper end of the hanging tape was placed on the lateral aspect of the left hepatic vein. The tape was then aligned with the ligamentum venosum. The position of the lower end of the hanging tape was determined according to left-sided hepatectomy type. The hanging tape gradually encircled either the left hepatic vein or the common trunk of the left hepatic vein and middle hepatic vein. The surgical procedures comprised: left lateral sectionectomy (n = 10); left hepatectomy (n = 17); and extended left hepatectomy including the middle hepatic vein (n = 2). Median operative time was 210 min (range 90-350 min). Median intraoperative blood loss was 200 ml (range 60-600 ml). Two intraoperative major hepatic vein injuries occurred during left hepatectomy. Neither patient developed massive bleeding or air embolism. Postoperative major complications occurred in one patient (3.4%). Median postoperative hospital stay was 7 days (range 4-15 days). No postoperative mortality occurred. The present modified liver hanging maneuver is a safe and effective method of outflow control during laparoscopic left-sided hepatectomy.

Acoustically-Equipped Ocean Gliders for Environmental and Oceanographic Research

DTIC Science & Technology

2015-09-30

and Oceanographic Research David K. Mellinger Oregon State University Hatfield Marine Science Center 2030 SE Marine Science Drive Newport, OR...www.bioacoustics.us LONG-TERM GOALS The long-term goal of this project is to establish acoustically-equipped gliders for use in research on marine ...funded research and proposed research. OBJECTIVES The Navy observes and studies marine mammals, both to better understand marine mammal

Determination of Ground Effect from Tests of a Glider in Towed Flight

NASA Technical Reports Server (NTRS)

Wetmore, J W; Turner, L I , Jr

1940-01-01

Report presents the results of an investigation made to find the effect of ground on the aerodynamic characteristics of a Franklin PS-2 glider. The lift, the drag, and the angle of attack of the glider in towed flight were determined at several heights from 0.14 to 1.19 span lengths and at various speeds for each height. Two wing arrangements were tested: the plain wing, and the wing with a nearly full-span 30-percent-chord split flap deflected 45 degrees. The experimental results for the plain wing were in good agreement with theoretical values calculated by the method of Wieselsberger for both the angle of attack and the drag coefficient at a height of 0.21 span length; Tani's refinements of the theory had a practically negligible effect on the computed values in this case.

Partly standing internal tides in a dendritic submarine canyon observed by an ocean glider

NASA Astrophysics Data System (ADS)

Hall, Rob A.; Aslam, Tahmeena; Huvenne, Veerle A. I.

2017-08-01

An autonomous ocean glider is used to make the first direct measurements of internal tides within Whittard Canyon, a large, dendritic submarine canyon system that incises the Celtic Sea continental slope and a site of high benthic biodiversity. This is the first time a glider has been used for targeted observations of internal tides in a submarine canyon. Vertical isopycnal displacement observations at different stations fit a one-dimensional model of partly standing semidiurnal internal tides - comprised of a major, incident wave propagating up the canyon limbs and a minor wave reflected back down-canyon by steep, supercritical bathymetry near the canyon heads. The up-canyon internal tide energy flux in the primary study limb decreases from 9.2 to 2.0 kW m-1 over 28 km (a dissipation rate of 1 - 2.5 ×10-7 Wkg-1), comparable to elevated energy fluxes and internal tide driven mixing measured in other canyon systems. Within Whittard Canyon, enhanced mixing is inferred from collapsed temperature-salinity curves and weakened dissolved oxygen concentration gradients near the canyon heads. It has previously been hypothesised that internal tides impact benthic fauna through elevated near-bottom current velocities and particle resuspension. In support of this, we infer order 20 cm s-1 near-bottom current velocities in the canyon and observe high concentrations of suspended particulate matter. The glider observations are also used to estimate a 1 °C temperature range and 12 μmol kg-1 dissolved oxygen concentration range, experienced twice a day by organisms on the canyon walls, due to the presence of internal tides. This study highlights how a well-designed glider mission, incorporating a series of tide-resolving stations at key locations, can be used to understand internal tide dynamics in a region of complex topography, a sampling strategy that is applicable to continental shelves and slopes worldwide.

The ultralight sailplane

NASA Technical Reports Server (NTRS)

Mcmasters, J. H.

1979-01-01

As presently envisioned, the ultralight sailplane is intermediate in size, cost and performance between current hang gliders and the lower end of the traditional sailplane spectrum. In the design of an ultralight sailplane, safety, low cost, and operational simplicity were emphasized at the expense of absolute performance. An overview of the design requirements for an ultralight sailplane is presented. It was concluded that by a judicious combination of the technologies of hang gliding, human powered flight, conventional soaring and motor gliding, an operationally and economically viable class of ultralight, self-launching sailplanes can be developed.

36 CFR 327.4 - Aircraft.

Code of Federal Regulations, 2014 CFR

2014-07-01

... GOVERNING PUBLIC USE OF WATER RESOURCE DEVELOPMENT PROJECTS ADMINISTERED BY THE CHIEF OF ENGINEERS § 327.4..., helicopters, ultra-light aircraft, motorized hang gliders, hot air balloons, any non-powered flight devices or..., material or equipment by parachute, balloon, helicopter or other means onto or from project lands or waters...

36 CFR 327.4 - Aircraft.

Code of Federal Regulations, 2012 CFR

2012-07-01

... GOVERNING PUBLIC USE OF WATER RESOURCE DEVELOPMENT PROJECTS ADMINISTERED BY THE CHIEF OF ENGINEERS § 327.4..., helicopters, ultra-light aircraft, motorized hang gliders, hot air balloons, any non-powered flight devices or..., material or equipment by parachute, balloon, helicopter or other means onto or from project lands or waters...

36 CFR 327.4 - Aircraft.

Code of Federal Regulations, 2011 CFR

2011-07-01

... GOVERNING PUBLIC USE OF WATER RESOURCE DEVELOPMENT PROJECTS ADMINISTERED BY THE CHIEF OF ENGINEERS § 327.4..., helicopters, ultra-light aircraft, motorized hang gliders, hot air balloons, any non-powered flight devices or..., material or equipment by parachute, balloon, helicopter or other means onto or from project lands or waters...

Resuspension and Shelf-Deep Ocean Exchange in the Northern California Current: New Insights From Underwater Gliders

NASA Astrophysics Data System (ADS)

Erofeev, A.; Barth, J. A.; Shearman, R. K.; Pierce, S. D.

2016-02-01

Shelf-deep ocean exchange is dominated by wind-driven upwelling and downwelling in the northern California Current. The interaction of strong, along-shelf jets with coastline and bottom topographic features can also create significant cross-margin exchange. We are using data from over 60,000 kilometers of autonomous underwater glider tracks to understand the temporal and spatial distribution of shelf-deep ocean exchange off central Oregon. Year-round glider observations of temperature, salinity, depth-averaged currents, chlorophyll fluorescence, light backscatter, and colored dissolved organic matter fluorescence from a single cross-margin transect are used to examine shelf-deep ocean exchange mechanisms. During summer, cross-margin exchange is dominated by wind-driven upwelling and the relaxation or reversal of the dominant southward winds. This process has been fairly well observed and studied due to the relatively low sea states and winds during summer. There is far less data from fall and winter off Oregon, a time of strong winds and large waves. We use autonomous underwater gliders to sample during the winter, including through the fall and spring transitions. Glider observations of suspended material detected via light backscatter, show time-space variations in resuspension in the bottom boundary layer due to winds, waves and currents. Examples of shelf-deep ocean exchange are shown by layers with high light backscatter separating from the bottom near the shelf break and extending into the interior along isopycnals. We describe these features and events in relationship to wind-forcing, along-shelf flows, and other forcing mechanisms.

Monitoring the Algerian Basin through glider observations, satellite altimetry and numerical simulations along a SARAL/AltiKa track

NASA Astrophysics Data System (ADS)

Aulicino, G.; Cotroneo, Y.; Ruiz, S.; Sánchez Román, A.; Pascual, A.; Fusco, G.; Tintoré, J.; Budillon, G.

2018-03-01

The Algerian Basin is a key component of the general circulation in the Western Mediterranean Sea. The presence of both fresh Atlantic water and more saline Mediterranean water gives the basin an intense inflow/outflow regime and complex circulation patterns. Energetic mesoscale structures that evolve from meanders of the Algerian Current into isolated cyclonic and anticyclonic eddies dominate the area, with marked repercussions on biological activity. Despite its remarkable importance, this region and its variability are still poorly known and basin-wide knowledge of its meso- and submesoscale features is still incomplete. Studying such complex processes requires a synergistic approach that involves integrated observing systems. In recent years, several studies have demonstrated the advantages of combined use of autonomous underwater vehicles, such as gliders, with a new generation of satellite altimetry. In this context, we present results of an observational program conducted in the Algerian Basin during fall 2014 and 2015 that aimed to advance our knowledge of its main features. The study was carried out through analysis of high resolution glider observations, collected along the Algerian BAsin Circulation Unmanned Survey (ABACUS) chokepoint, in synergy with co-located SARAL/AltiKa altimetric products and CMEMS numerical simulations. Results show that glider-derived dynamic height and SARAL/AltiKa absolute dynamic topography have similar patterns, with RMS of the differences ranging between 1.11 and 2.90 cm. Even though larger discrepancies are observed near the Balearic and Algerian coasts, correlation coefficients between glider and satellite observations seem mostly to be affected by reduced synopticity between the measurements. Glider observations acquired during the four surveys reveal the presence of several water masses of Atlantic and Mediterranean origin (i.e., AW and LIW at different modification levels) with marked seasonal variability.

Langley Full-scale-tunnel Investigation of Maximum Lift and Stability Characteristics of an Airplane Having Approximately Triangular Plan Form (DM-1 Glider)

NASA Technical Reports Server (NTRS)

Lovell, J Calvin; Wilson, Herbert A JR

1947-01-01

An investigation of the DM-1 Glider, which had approximately triangular plan form, an aspect ratio of 1.8 and a 60 degree sweptback leading edge, has been conducted in the Langley full-scale tunnel. The investigation consisted of the determination of the separate effects of the following modifications made to the glider on its maximum lift and stability characteristics: (a) installation of sharp leading edges over the inboard semispan of the wing, (b) removal of the vertical fin, (c) sealing of the elevon control-balance slots, (d) installation of redesigned thin vertical surfaces, (e) installation of faired sharp leading edges, and (f) installation of canopy. The maximum lift coefficient of the DM-1 glider was increased from 0.61 to 1.01 by the installation of semispan sharp leading edges, and from 1.01 to 1.24 by the removal of the vertical fin and sealing of the elevon control-balance slots. The highest maximum lift coefficient (1.32) was obtained when the faired sharp leading edges and the thin vertical surfaces were attached to the glider. The original DM-1 glider was longitudinally stable. The semispan sharp leading edges shifted the neutral point forward approximately 3 percent of the root chord at moderate lift coefficients, and the glider configuration with these sharp leading edges attached was longitudinally unstable, for the assumed center-of-gravity location, at lift coefficients above 0.73. Sealing the elevon control-balance slots and installing the faired sharp leading edges, the thin vertical surfaces, and the canopy shifted the neutral point forward approximately 8 percent of the root chord.

HangOut: generating clean PSI-BLAST profiles for domains with long insertions.

PubMed

Kim, Bong-Hyun; Cong, Qian; Grishin, Nick V

2010-06-15

Profile-based similarity search is an essential step in structure-function studies of proteins. However, inclusion of non-homologous sequence segments into a profile causes its corruption and results in false positives. Profile corruption is common in multidomain proteins, and single domains with long insertions are a significant source of errors. We developed a procedure (HangOut) that, for a single domain with specified insertion position, cleans erroneously extended PSI-BLAST alignments to generate better profiles. HangOut is implemented in Python 2.3 and runs on all Unix-compatible platforms. The source code is available under the GNU GPL license at http://prodata.swmed.edu/HangOut/. Supplementary data are available at Bioinformatics online.

Geometric effects resulting from the asymmetry of dipping fault: Hanging wall/ footwall effects

NASA Astrophysics Data System (ADS)

Wang, Dong; Xie, Li-Li; Hu, Jin-Jun

2008-05-01

Root-mean-square distance D rms with characteristic of weighted-average is introduced in this article firstly. D rms can be used to capture the general proximity of a site to a dipping fault plane comparing with the rupture distance D rup and the seismogenic distance D seis. Then, using D rup, D seis and D rms, the hanging wall/footwall effects on the peak ground acceleration (PGA) during the 1999 Chi-Chi earthquake are evaluated by regression analysis. The logarithm residual shows that the PGA on hanging wall is much greater than that on footwall at the same D rup or D seis when the D rup or D seis is used as site-to-source distance measure. In contrast, there is no significant difference between the PGA on hanging wall and that on footwall at the same D rms when D rms is used. This result confirms that the hanging wall/footwall effect is mainly a geometric effect caused by the asymmetry of dipping fault. Therefore, the hanging wall/footwall effect on the near-fault ground motions can be ignored in the future attenuation analysis if the root-mean-square distance D rms is used as the site-to-source distance measure.

76 FR 5 - Feathering Propeller Systems for Light-Sport Aircraft Powered Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-03

... and Procedures of the Department of Transportation (DOT) (44 FR 1134, February 26, 1979) provide that... Soaring Flight When we published the notice of proposed rulemaking (NPRM) entitled Certification of... powered gliders to decrease drag while soaring. In June 2008, the Light Aircraft Manufacturers Association...

Zoonoses of ferrets, hedgehogs, and sugar gliders.

PubMed

Pignon, Charly; Mayer, Jörg

2011-09-01

With urbanization, people live in close proximity to their pets. People often share their living quarters and furniture, and this proximity carries a new potential for pathogen transmission. In addition to the change in lifestyle with our pets, new exotic pets are being introduced to the pet industry regularly. Often, we are unfamiliar with specific clinical signs of diseases in these new exotic pets or the routes of transmission of pathogens for the particular species. This article reviews zoonoses that occur naturally in ferrets, hedgehogs, and sugar gliders, discussing the occurrence and clinical symptoms of these diseases in humans.

Ocean glider observations of iceberg-enhanced biological production in the northwestern Weddell Sea

NASA Astrophysics Data System (ADS)

Biddle, Louise C.; Kaiser, Jan; Heywood, Karen J.; Thompson, Andrew F.; Jenkins, Adrian

2015-01-01

Icebergs affect local biological production around Antarctica. We used an ocean glider to observe the effects of a large iceberg that was advected by the Antarctic Slope Current along the continental slope in the northwestern Weddell Sea in early 2012. The high-resolution glider data reveal a pronounced effect of the iceberg on ocean properties, with oxygen concentrations of (13 ± 4) μmol kg-1 higher than levels in surrounding waters, which are most likely due to positive net community production. This response was confined to three areas of water in the direct vicinity of the iceberg track, each no larger than 2 km2. Our findings suggest that icebergs have an impact on Antarctic production presumably through local micronutrient injections, on a scale smaller than typical satellite observations of biological production in the Southern Ocean.

Characterization of Convective Plumes Associated With Oceanic Deep Convection in the Northwestern Mediterranean From High-Resolution In Situ Data Collected by Gliders

NASA Astrophysics Data System (ADS)

Margirier, Félix; Bosse, Anthony; Testor, Pierre; L'Hévéder, Blandine; Mortier, Laurent; Smeed, David

2017-12-01

Numerous gliders have been deployed in the Gulf of Lions (northwestern Mediterranean Sea) and in particular during episodes of open-ocean deep convection in the winter 2012-2013. The data collected represents an unprecedented density of in situ observations providing a first in situ statistical and 3-D characterization of the important mixing agents of the deep convection phenomenon, the so-called plumes. A methodology based on a glider-static flight model was applied to infer the oceanic vertical velocity signal from the glider navigation data. We demonstrate that during the active phase of mixing, the gliders underwent significant oceanic vertical velocities up to 18 cm s-1. Focusing on the data collected by two gliders during the 2012-2013 winter, 120 small-scale convective downward plumes were detected with a mean radius of 350 m and separated by about 2 km. We estimate that the plumes cover 27% of the convection area. Gliders detected downward velocities with a magnitude larger than that of the upward ones (-6 versus +2 cm s-1 on average). Along-track recordings of temperature and salinity as well as biogeochemical properties (dissolved oxygen, fluorescence, and turbidity) allow a statistical characterization of the water masses' properties in the plumes' core with respect to the "background": the average downward signal is of colder (-1.8 × 10-3 °C), slightly saltier (+4.9 × 10-4 psu) and thus denser waters (+7.5 × 10-4 kg m-3). The plunging waters are also on average more fluorescent (+2.3 × 10-2 μg L-1). The plumes are associated with a vertical diffusion coefficient of 7.0 m2 s-1 and their vertical velocity variance scales with the ratio of the buoyancy loss over the Coriolis parameter to the power 0.86.

Seafloor Geodesy usi­ng Wave Gliders to study Earthquake and Tsunami Hazards at Subduction Zones

NASA Astrophysics Data System (ADS)

Sathiakumar, S.; Barbot, S.; Hill, E.; Peng, D.; Zerucha, J.; Suhaimee, S.; Chia, G.; Salamena, G. G.; Syahailatua, A.

2016-12-01

Land-based GNSS networks are now in place to monitor most subduction zones of the world. These provide valuable information about the amount of­ geodetic strain accumulated in the region, which in turn gives insight into the seismic potential. However, it is usually impossible to resolve activity on the megathrust near the trench using land-based GNSS data alone, given typical signal-to-noise ratios. Ship-based seafloor geodesy is being used today to fill this observation gap. However, surveys using ships are very expensive, tedious and impractical due to the large areas to be covered. Instead of discrete missions using ships, continuous monitoring of the seafloor using autonomous marine robots would aid in understanding the tectonic setting of the seafloor better at a potentially lower cost, as well as help in designing better warning systems. Thus, we are developing seafloor geodesy capabilities using Wave Gliders, a new class of wave-propelled, persistent marine autonomous vehicle using a combination of acoustic and GNSS technologies. We use GNSS/INS to position the platform, and acoustic ranging to locate the seafloor. The GNSS/INS system to be integrated with the Wave Gliders has stringent requirements of low power, light weight, and high accuracy. All these factors are equally important due to limited power and space in the Wave Gliders and the need for highly accurate and precise measurements. With this hardware setup, a limiting factor is the accuracy of measurement of the sound velocity in the water column. We plan to obtain precise positioning of seafloor by exploring a measurement setup that minimizes uncertainties in sound velocity. This will be achieved by making fine-resolution measurements of the two-way travel time of the acoustic waves underwater using the Wave Gliders, and performing statistical signal processing on this data to obtain more reliable sound velocity measurement. This enhanced seafloor geodetic technique using Wave Gliders should

Effects of helium/oxygen and temperature on aerobic metabolism in the marsupial sugar glider, Petaurus breviceps.

PubMed

Holloway, J C; Geiser, F

2001-01-01

Helox (79% helium and 21% oxygen) has often been used for thermobiological studies, primarily because helium is thought to be metabolically inert and to produce no adverse effects other than increasing heat loss. However, these assumptions have been questioned. As basal metabolic rate (BMR) represents maintenance energy requirements for vital body functions, potential physiological effects of helox should be reflected in changes of BMR. In this study, sugar gliders were subjected to both air and helox atmospheres over a wide range of T(a)'s, including the thermoneutral zone (TNZ), to determine (1) whether helox has any influence other than on heat loss and (2) the maximum heat production (HP(max)) and thermal limits of this species. Although thermal conductance in the TNZ increased in helox, BMR was similar in air and helox (0.55+/-0.07 and 0.57+/-0.06 mL g(-1) h(-1), respectively). The TNZ in helox, however, was shifted upwards by about 3 degrees C. Below the TNZ, sugar gliders were able to withstand an effective temperature of -24.7+/-7.3 degrees C with an HP(max) of 3.14+/-0.36 mL g(-1) h(-1). The low effective temperature tolerated by sugar gliders shows that they are competent thermoregulators despite their apparent lack of functional brown fat. Similarities of BMRs in air and helox suggest that the effect of helox is restricted to an increase of heat loss, and, consequently, helox represents a useful tool for thermal physiologists. Moreover, the lack of increase of BMR in helox despite an increase in thermal conductance of sugar gliders suggests that BMR is not a function of body surface.

A Low Cost Approach to the Design of Autopilot for Hypersonic Glider

NASA Astrophysics Data System (ADS)

Liang, Wang; Weihua, Zhang; Ke, Peng; Donghui, Wang

2017-12-01

This paper proposes a novel integrated guidance and control (IGC) approach to improve the autopilot design with low cost for hypersonic glider in dive and pull-up phase. The main objective is robust and adaptive tracking of flight path angle (FPA) under severe flight scenarios. Firstly, the nonlinear IGC model is developed with a second order actuator dynamics. Then the adaptive command filtered back-stepping control is implemented to deal with the large aerodynamics coefficient uncertainties, control surface uncertainties and unmatched time-varying disturbances. For the autopilot, a back-stepping sliding mode control is designed to track the control surface deflection, and a nonlinear differentiator is used to avoid direct differentiating the control input. Through a series of 6-DOF numerical simulations, it’s shown that the proposed scheme successfully cancels out the large uncertainties and disturbances in tracking different kinds of FPA trajectory. The contribution of this paper lies in the application and determination of nonlinear integrated design of guidance and control system for hypersonic glider.

Radiation profiles through the atmosphere measured by an auto controlled glider aircraft

NASA Astrophysics Data System (ADS)

Kräuchi, Andreas; Philipona, Rolf

2014-05-01

In 2011 radiation measurements through the atmosphere were made with a balloon borne short- and longwave net radiometer. These measurements were very promising and therefore new and improved sensors from Kipp&Zonen were used to equip a glider aircraft together with the standard Swiss radiosonde from Meteolabor AG. The glider serves as returning platform for the expensive and well calibrated radiation sensors. Double balloon technique is used to prevent pendulum motion during the ascent and to keep the radiation instruments as horizontal as possible. The built-in autopilot allows to return the gliderradiosonde to the launch site or to land it on predefined open space, which makes recovery much easier. The new return gliderradiosonde technique as well as new measurement possibilities will be shown. First measurements show radiation profiles through the atmosphere during different cloud conditions. Radiation profiles during different daytimes show the temporal resolution of vertical radiation profiles trough the atmosphere.

Adding the 'heart' to hanging drop networks for microphysiological multi-tissue experiments.

PubMed

Rismani Yazdi, Saeed; Shadmani, Amir; Bürgel, Sebastian C; Misun, Patrick M; Hierlemann, Andreas; Frey, Olivier

2015-11-07

Microfluidic hanging-drop networks enable culturing and analysis of 3D microtissue spheroids derived from different cell types under controlled perfusion and investigating inter-tissue communication in multi-tissue formats. In this paper we introduce a compact on-chip pumping approach for flow control in hanging-drop networks. The pump includes one pneumatic chamber located directly above one of the hanging drops and uses the surface tension at the liquid-air-interface for flow actuation. Control of the pneumatic protocol provides a wide range of unidirectional pulsatile and continuous flow profiles. With the proposed concept several independent hanging-drop networks can be operated in parallel with only one single pneumatic actuation line at high fidelity. Closed-loop medium circulation between different organ models for multi-tissue formats and multiple simultaneous assays in parallel are possible. Finally, we implemented a real-time feedback control-loop of the pump actuation based on the beating of a human iPS-derived cardiac microtissue cultured in the same system. This configuration allows for simulating physiological effects on the heart and their impact on flow circulation between the organ models on chip.

An Autonomous Glider Network for the Monterey Bay Predictive Skill Experiment / AOSN-II

DTIC Science & Technology

2006-12-13

distributed measurements (Figure 1) of temperature and salinity, vertically-averaged velocity, chlorophyll fluorescence , optical backscatter, and PAR. We... Clarence Rowley, and Jerrold Marsden N000140210826 Underwater Glider Dynamics and Control Leonard (PI) N00014-02-1-0861 Autonomous Ocean Sampling

An updated system for guidance of heterogeneous platforms used for multiple gliders in a real-time experiment

NASA Astrophysics Data System (ADS)

Smedstad, L.; Barron, C. N.; Book, J. W.; Osborne, J. J.; Souopgui, I.; Rice, A. E.; Linzell, R. S.

2017-12-01

The Guidance of Heterogeneous Observation Systems (GHOST) is a tool designed to sample ocean model outputs to determine a suite of possible path options for unmanned platforms. The system is built around a Runge-Kutta method to determine all possible paths, followed by a cost function calculation, an enforcement of safe operating area, and an analysis to determine a top 10% level of cost function and to rank the paths that qualify. A field experiment took place from 16 May until 5 June 2017 aboard the R/V Savannah operating out of the Duke University Marine Laboratory (DUML) in Beaufort, NC. Gliders were deployed in alternating groups with missions defined by one of two possible categories: a station-keeping array and a moving array. Unlike previous versions of the software, which monitored platforms individually, these gliders were placed in groups of 2-5 gliders with the same tasks. Daily runs of the GHOST software were performed for each mission category and for two different 1 km orientations of the Navy Coastal Ocean Model (NCOM). By limiting the number of trial solutions and by sorting through the best results, a quick turnaround was made possible for glider operators to determine waypoints in order to remain in desired areas or to move in paths that sampled areas of highest thermohaline variability. Limiting risk by restricting solutions to defined areas with statistically less likely occurrences of high ocean currents was an important consideration in this study area that was located just inshore of the Gulf Stream.

Shelf Sea Oxygen Dynamics: A year of Glider Measurements

NASA Astrophysics Data System (ADS)

Williams, C. A. J.; Palmer, M.; Mahaffey, C.; Jardine, J.

2016-02-01

Oxygen (O) is involved in most biogeochemical processes in the ocean, and dissolved oxygen (DO) is a well-established indicator for biological activity via the estimate of apparent oxygen utilisation (AOU). In the deep waters of the open ocean, the AOU provides a valuable insight into the ocean's biological carbon pump. However, in the physically dynamic and highly productive shallow shelf seas, interpretation of the O distribution and the magnitude of AOU is complex. Physical processes, such as diapycnal mixing, entrainment and horizontal advection act to ventilate waters below the thermocline and thus increase O and decrease AOU. In contrast, biological remineralisation of organic material below the thermocline will consume O and increase AOU. Here, we use 1 year of high-resolution data from >20 glider deployments in the seasonally stratified NW European Shelf Sea to identify and quantify the physical and biological processes that control the DO distribution and magnitude of AOU in shelf seas. A 200km transect between the shelf edge and the central Celtic Sea (CCS) was repeated between November 2014 and August 2015, thus capturing key periods in the seasonal cycling in shelf seas, specifically the onset of stratification, the spring bloom, stratified summer period and breakdown of stratification. The gliders collected data for DO, temperature, salinity, chlorophyll fluorescence, CDOM, backscatter and turbulence. In addition, direct measurements of turbulent dissipation from the Ocean Microstructure Glider deployed during the campaign provided estimates of mixing at CCS and the shelf break, allowing accurate quantification of the vertical fluxes of O. At the end of the stratified period the DO concentration was higher and AOU was lower at the shelf break (80 µM) compared to at CCS on shelf (>95 µM) (Fig 1). Estimates of vertical DO fluxes indicate that this horizontal variation in DO and AOU was partly attributed to enhanced mixing via internal waves at the

Some Landing Studies Pertinent to Glider-Reentry Vehicles

NASA Technical Reports Server (NTRS)

Houbolt, John C.; Batterson, Sidney A.

1960-01-01

Results are presented of some landing studies that may serve as guidelines in the consideration of landing problems of glider-reentry configurations. The effect of the initial conditions of sinking velocity, angle of attack, and pitch rate on impact severity and the effect of locating the rear gear in various positions are discussed. Some information is included regarding the influence of landing-gear location on effective masses. Preliminary experimental results on the slideout phase of landing include sliding and rolling friction coefficients that have been determined from tests of various skids and all-metal wheels.

Evaluation of Vitality in the Experimental Hanging Model of Rats by Using Immunohistochemical IL-1β Antibody Staining.

PubMed

Balandiz, Hüseyin; Pehlivan, Sultan; Çiçek, Ali Fuat; Tuğcu, Harun

2015-12-01

Hanging is the most common suicide method in the world, and the discrimination of antemortem-postmortem hanging must be done at autopsy. The aim of this experimental study was to examine the immunohistochemical expression of IL-1β antibody at the hanging mark skin samples of rats to discriminate antemortem and postmortem hangings. A total of 20 Wistar albino rats were used for this study. The groups were as follows: A-1, antemortem control group; A-2, antemortem second-hour hanging mark skin samples; A-3, antemortem 24th-hour hanging mark skin samples; A-4, antemortem 72nd-hour hanging mark skin samples; B-1, postmortem control group; and B-2, postmortem second-hour hanging mark skin samples. Interleukin-1β immunostaining was performed to all tissue samples. For epidermal cells, group A-1 samples did not show IL-1β immunostaining, group A-2 samples were severely immunostained, and groups A-3 and A-4 samples' staining were slightly decreased. There was no IL-1β antibody staining in groups B-1 and B-2 samples. For adnexal cells, groups A-1 and B-1 samples did not show IL-1β immunostaining, staining of group A-2 samples was mild to severe, and groups A-3 and A-4 samples' staining were slightly decreased. Half of the group B-2 samples did not show IL-1β immunostaining. For subepidermal cells, most of the samples of groups A-1 and B-1 showed slight immunostaining, groups A-2 and B-2 samples' staining were mild to severe, and there were slight immunostaining in groups A-3 and A-4 samples. The majority of vascular structure cells did not show IL-1β immunostaining. Interleukin-1β immunostaining of epidermal cells can discriminate antemortem-postmortem hangings, but vascular structure cells and subepidermal cells cannot discriminate vital hangings.

Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis.

PubMed

Frey, Olivier; Misun, Patrick M; Fluri, David A; Hengstler, Jan G; Hierlemann, Andreas

2014-06-30

Integration of multiple three-dimensional microtissues into microfluidic networks enables new insights in how different organs or tissues of an organism interact. Here, we present a platform that extends the hanging-drop technology, used for multi-cellular spheroid formation, to multifunctional complex microfluidic networks. Engineered as completely open, 'hanging' microfluidic system at the bottom of a substrate, the platform features high flexibility in microtissue arrangements and interconnections, while fabrication is simple and operation robust. Multiple spheroids of different cell types are formed in parallel on the same platform; the different tissues are then connected in physiological order for multi-tissue experiments through reconfiguration of the fluidic network. Liquid flow is precisely controlled through the hanging drops, which enable nutrient supply, substance dosage and inter-organ metabolic communication. The possibility to perform parallelized microtissue formation on the same chip that is subsequently used for complex multi-tissue experiments renders the developed platform a promising technology for 'body-on-a-chip'-related research.

A PDMS-Based Microfluidic Hanging Drop Chip for Embryoid Body Formation.

PubMed

Wu, Huei-Wen; Hsiao, Yi-Hsing; Chen, Chih-Chen; Yet, Shaw-Fang; Hsu, Chia-Hsien

2016-07-06

The conventional hanging drop technique is the most widely used method for embryoid body (EB) formation. However, this method is labor intensive and limited by the difficulty in exchanging the medium. Here, we report a microfluidic chip-based approach for high-throughput formation of EBs. The device consists of microfluidic channels with 6 × 12 opening wells in PDMS supported by a glass substrate. The PDMS channels were fabricated by replicating polydimethyl-siloxane (PDMS) from SU-8 mold. The droplet formation in the chip was tested with different hydrostatic pressures to obtain optimal operation pressures for the wells with 1000 μm diameter openings. The droplets formed at the opening wells were used to culture mouse embryonic stem cells which could subsequently developed into EBs in the hanging droplets. This device also allows for medium exchange of the hanging droplets making it possible to perform immunochemistry staining and characterize EBs on chip.

Application of hanging drop technique to optimize human IgG formulations.

PubMed

Li, Guohua; Kasha, Purna C; Late, Sameer; Banga, Ajay K

2010-01-01

The purpose of this work is to assess the hanging drop technique in screening excipients to develop optimal formulations for human immunoglobulin G (IgG). A microdrop of human IgG and test solution hanging from a cover slide and undergoing vapour diffusion was monitored by a stereomicroscope. Aqueous solutions of IgG in the presence of different pH, salt concentrations and excipients were prepared and characterized. Low concentration of either sodium/potassium phosphate or McIlvaine buffer favoured the solubility of IgG. Addition of sucrose favoured the stability of this antibody while addition of NaCl caused more aggregation. Antimicrobial preservatives were also screened and a complex effect at different buffer conditions was observed. Dynamic light scattering, differential scanning calorimetry and size exclusion chromatography studies were performed to further validate the results. In conclusion, hanging drop is a very easy and effective approach to screen protein formulations in the early stage of formulation development.

Optimizing observational networks combining gliders, moored buoys and FerryBox in the Bay of Biscay and English Channel

NASA Astrophysics Data System (ADS)

Charria, Guillaume; Lamouroux, Julien; De Mey, Pierre

2016-10-01

Designing optimal observation networks in coastal oceans remains one of the major challenges towards the implementation of future efficient Integrated Ocean Observing Systems to monitor the coastal environment. In the Bay of Biscay and the English Channel, the diversity of involved processes (e.g. tidally-driven circulation, plume dynamics) requires to adapt observing systems to the specific targeted environments. Also important is the requirement for those systems to sustain coastal applications. Two observational network design experiments have been implemented for the spring season in two regions: the Loire River plume (northern part of the Bay of Biscay) and the Western English Channel. The method used to perform these experiments is based on the ArM (Array Modes) formalism using an ensemble-based approach without data assimilation. The first experiment in the Loire River plume aims to explore different possible glider endurance lines combined with a fixed mooring to monitor temperature and salinity. Main results show an expected improvement when combining glider and mooring observations. The experiment also highlights that the chosen transect (along-shore and North-South, cross-shore) does not significantly impact the efficiency of the network. Nevertheless, the classification from the method results in slightly better performances for along-shore and North-South sections. In the Western English Channel, a tidally-driven circulation system, added value of using a glider below FerryBox temperature and salinity measurements has been assessed. FerryBox systems are characterised by a high frequency sampling rate crossing the region 2 to 3 times a day. This efficient sampling, as well as the specific vertical hydrological structure (which is homogeneous in many sub-regions of the domain), explains the fact that the added value of an associated glider transect is not significant. These experiments combining existing and future observing systems, as well as numerical

78 FR 26241 - Airworthiness Directives; Diamond Aircraft Industries GmbH Powered Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-06

... Airworthiness Directives; Diamond Aircraft Industries GmbH Powered Gliders AGENCY: Federal Aviation... directive (AD) for certain Diamond Aircraft Industries GmbH Models HK 36 R, HK 36 TS, and HK 36 TTS powered... removing the Model H-36 from the Applicability. This AD was prompted by reports of installation of an...

INTERIOR OF COLD STORAGE ROOM, SHOWING MOVABLE HANGING RACKS. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

INTERIOR OF COLD STORAGE ROOM, SHOWING MOVABLE HANGING RACKS. - Naval Air Station Barbers Point, Aircraft Storehouse, Between Midway & Card Streets at Enterprise Avenue intersection, Ewa, Honolulu County, HI

Expanding Alaska's Remote Ocean Observing Capabilities Using Robotic Gliders and Remote Sensing Technologies

NASA Astrophysics Data System (ADS)

Janzen, C.; McCammon, M.; Winsor, P.; Murphy, D. J.; Mathis, J. T.; Baumgartner, M.; Stafford, K.; Statscewich, H.; Evans, W.; Potter, R. A.

2016-02-01

The Alaska Ocean Observing System (AOOS) is directed by Congress to facilitate, implement and support ocean observing for the entire coast of Alaska, working with federal, state, local and private sector partners. However, developing an integrated ocean observing system at high latitudes presents unique challenges. In addition to the harsh environment, the region covered by AOOS is made up of nearly 44,000 miles of coastline, larger than the marine systems in the rest of the United States combined. No other observing system in the United States has such climate extremes, significant geographic distances, and limited observing infrastructure. Making use of robotic technologies in Alaskan waters has been successfully demonstrated with the pilot deployment of a real-time marine mammal detection system deployed on a Slocum buoyancy controlled glider. The glider also carries payload to measure high resolution temperature and salinity data. With these simultaneous data streams, scientists are investigating how marine mammal occurrences are related to water column conditions and mixing fronts, as well as comparing northern versus southern Chukchi community composition, inshore (Alaska Coastal Current) waters, and offshore (Bering Sea) waters. In its third year, the glider is now equipped with lithium batteries that allow it to operate unattended for an entire Arctic summer season, whereas past deployments were limited to about 10 days. Developing and applying such cutting edge, long-endurance autonomous technology is benefitting others monitoring in Arctic regions where shipboard access is not only expensive, but limited to fair weather conditions during the openwater (ice free) seasons of summer to early fall.

Upper-ocean Response to Hurricane Gonzalo (2014): Salinity Effects Revealed by Targeted and Sustained Underwater Glider Observation

NASA Astrophysics Data System (ADS)

Domingues, R. M.; Goni, G. J.; Bringas, F.; Lee, S. K.; Kim, H. S. S.; Halliwell, G. R., Jr.; Dong, J.; Morell, J. M.; Pomales, L.

2016-02-01

In July 2014, two underwater gliders were deployed off Puerto Rico as part of a multi-institutional effort lead by NOAA/AOML funded by the Disaster Appropriations Relief Act of 2013 known as Sandy Supplemental. The goal of this work is to collect ocean observations to: (1) investigate the response of the ocean to tropical cyclone (TC) wind conditions; (2) improve understanding on the role that the ocean plays in the intensification of TCs; and (3) help improve TC seasonal and intensity forecasts. The two gliders were piloted along predetermined tracks in the Caribbean Sea and in the North Atlantic Ocean (Figure 1), where TCs very often travel and intensify. On October 12, 2014, TC Gonzalo developed in the tropical North Atlantic, reaching the status of Category 3 hurricane on October 14 as it travelled 85 km northeast of the location of the glider (site B, Figure 1). The sampling strategy adopted during the passage of Hurricane Gonzalo consisted of carrying out observations: along a repeat section three times between sites A and B, one before and two after the passage of the hurricane; and at a fixed location at site B during the passage of the hurricane. Observations collected before, during, and after the passage of this hurricane were analyzed to improve our understanding of the upper-ocean response to hurricane winds. The main finding in this study is that salinity played an important role on the upper-ocean response to Hurricane Gonzalo; where a near-surface barrier-layer has likely suppressed the hurricane-induced upper-ocean cooling, leading to smaller than expected temperature changes of -0.4°C. Post-storm observations also revealed a partial recovery of the ocean to pre-storm conditions 11 days after the hurricane. Glider observations were further compared with outputs from a numerical coupled atmospheric-ocean model used for hurricane prediction to evaluate the model performance in simulating the upper-ocean response during Hurricane Gonzalo. The

Measuring structure deformations of a composite glider by optical means with on-ground and in-flight testing

NASA Astrophysics Data System (ADS)

Bakunowicz, Jerzy; Święch, Łukasz; Meyer, Ralf

2016-12-01

In aeronautical research experimental data sets of high quality are essential to verify and improve simulation algorithms. For this reason the experimental techniques need to be constantly refined. The shape, movement or deformation of structural aircraft elements can be measured implicitly in multiple ways; however, only optical, correlation-based techniques are able to deliver direct high-order and spatial results. In this paper two different optical metrologies are used for on-ground preparation and the actual execution of in-flight wing deformation measurements on a PW-6U glider. Firstly, the commercial PONTOS system is used for static tests on the ground and for wind tunnel investigations to successfully certify an experimental sensor pod mounted on top of the test bed fuselage. Secondly, a modification of the glider is necessary to implement the optical method named image pattern correlation technique (IPCT), which has been developed by the German Aerospace Center DLR. This scientific technology uses a stereoscopic camera set-up placed inside the experimental pod and a stochastic dot matrix applied to the area of interest on the glider wing to measure the deformation of the upper wing surface in-flight. The flight test installation, including the preparation, is described and results are presented briefly. Focussing on the compensation for typical error sources, the paper concludes with a recommended procedure to enhance the data processing for better results. Within the presented project IPCT has been developed and optimized for a new type of test bed. Adapted to the special requirements of the glider, the IPCT measurements were able to deliver a valuable wing deformation data base which now can be used to improve corresponding numerical models and simulations.

A hanging drop culture method to study terminal erythroid differentiation.

PubMed

Gutiérrez, Laura; Lindeboom, Fokke; Ferreira, Rita; Drissen, Roy; Grosveld, Frank; Whyatt, David; Philipsen, Sjaak

2005-10-01

To design a culture method allowing the quantitative and qualitative analysis of terminal erythroid differentiation. Primary erythroid progenitors derived either from mouse tissues or from human umbilical cord blood were differentiated using hanging drop cultures and compared to methylcellulose cultures. Cultured cells were analyzed by FACS to assess differentiation. We describe a practical culture method by adapting the previously described hanging drop culture system to conditions allowing terminal differentiation of primary erythroid progenitors. Using minimal volumes of media and small numbers of cells, we obtained quantitative terminal erythroid differentiation within two days of culture in the case of murine cells and 4 days in the case of human cells. The established methods for ex vivo culture of primary erythroid progenitors, such as methylcellulose-based burst-forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E) assays, allow the detection of committed erythroid progenitors but are of limited value to study terminal erythroid differentiation. We show that the application of hanging drop cultures is a practical alternative that, in combination with clonogenic assays, enables a comprehensive assessment of the behavior of primary erythroid cells ex vivo in the context of genetic and drug-induced perturbations.

Survey of submesoscale structures at the margin of the Northern Current in the North Western Mediterranean Sea using Gliders: observations and diagnostics

NASA Astrophysics Data System (ADS)

Bosse, Anthony; Testor, Pierre; Mortier, Laurent; Beguery, Laurent; Bernardet, Karim; Taillandier, Vincent; d'Ortenzio, Fabrizio; Prieur, Louis; Coppola, Laurent; Bourrin, François

2013-04-01

From 2008 on, repeated sections crossing the Northern Current (NC) were operated by gliders as part of a global observing system (MOOSE project) of the North Western Mediterranean Sea. This work is dedicated to the analysis of the submesoscale thermohaline variability at the margin of this current observed by gliders. The mean circulation of the basin is characterized by a cyclonic gyre (whose Northern part is the so-called NC) associated with a doming of the isopycnals preconditionning the whole interior basin to great vertical mixing. The thermal and haline differences between the Atlantic Water (AW) transported by the NC and older and modified AW off the coast leads to a frontal structure. Especially in winter, when the mixed layer depth used to reach several hundreds of meters offshore, isopycnal outcropping and the role of frontal processes are enhanced leading to intense variability at scales smaller than the deformation radius. Based on diagnostics using the Potential Vorticity (PV) computed from the glider data assuming quasi-geostrophic conditions and no variation in the alongshore direction, we discuss the dynamical processes at work, with a focus on 2 typical examples: (1) the first example takes place in winter during a strong vertical mixing event. While the glider crossed the frontal region, the temperature and salinity fields exhibit vertical motions at depths about 0-400m. Frontogenesis might be at play through mesoscale strain since the glider shows an intense mesoscale activity but a weak stratification and enhanced horizontal buoyancy gradient actually make the Ertel PV reach negative values and symmetric instability is likely to be a prominent mechanism explaining the observed variability. (2) the second example takes place in spring. We identify an episode of down-front wind blowing during the glider deployment which could have extracted PV from the surface layer. However, the geostrophic turbulence is in that case likely to play a key role in

PLOCAN glider portal: a gateway for useful data management and visualization system

NASA Astrophysics Data System (ADS)

Morales, Tania; Lorenzo, Alvaro; Viera, Josue; Barrera, Carlos; José Rueda, María

2014-05-01

Nowadays monitoring ocean behavior and its characteristics involves a wide range of sources able to gather and provide a vast amount of data in spatio-temporal scales. Multiplatform infrastructures, like PLOCAN, hold a variety of autonomous Lagrangian and Eulerian devices addressed to collect information then transferred to land in near-real time. Managing all this data collection in an efficient way is a major issue. Advances in ocean observation technologies, where underwater autonomous gliders play a key role, has brought as a consequence an improvement of spatio-temporal resolution which offers a deeper understanding of the ocean but requires a bigger effort in the data management process. There are general requirements in terms of data management in that kind of environments, such as processing raw data at different levels to obtain valuable information, storing data coherently and providing accurate products to final users according to their specific needs. Managing large amount of data can be certainly tedious and complex without having right tools and operational procedures; hence automating these tasks through software applications saves time and reduces errors. Moreover, data distribution is highly relevant since scientist tent to assimilate different sources for comparison and validation. The use of web applications has boosted the necessary scientific dissemination. Within this argument, PLOCAN has implemented a set of independent but compatible applications to process, store and disseminate information gathered through different oceanographic platforms. These applications have been implemented using open standards, such as HTML and CSS, and open source software, like python as programming language and Django as framework web. More specifically, a glider application has been developed within the framework of FP7-GROOM project. Regarding data management, this project focuses on collecting and making available consistent and quality controlled datasets as

Wireless Orbiter Hang-Angle Inclinometer System

NASA Technical Reports Server (NTRS)

Lucena, Angel; Perotti, Jose; Green, Eric; Byon, Jonathan; Burns, Bradley; Mata, Carlos; Randazzo, John; Blalock, Norman

2011-01-01

A document describes a system to reliably gather the hang-angle inclination of the orbiter. The system comprises a wireless handheld master station (which contains the main station software) and a wireless remote station (which contains the inclinometer sensors, the RF transceivers, and the remote station software). The remote station is designed to provide redundancy to the system. It includes two RF transceivers, two power-management boards, and four inclinometer sensors.

Characterization and reproducibility of HepG2 hanging drop spheroids toxicology in vitro.

PubMed

Hurrell, Tracey; Ellero, Andrea Antonio; Masso, Zelie Flavienne; Cromarty, Allan Duncan

2018-02-21

Hepatotoxicity remains a major challenge in drug development despite preclinical toxicity screening using hepatocytes of human origin. To overcome some limitations of reproducing the hepatic phenotype, more structurally and functionally authentic cultures in vitro can be introduced by growing cells in 3D spheroid cultures. Characterisation and reproducibility of HepG2 spheroid cultures using a high-throughput hanging drop technique was performed and features contributing to potential phenotypic variation highlighted. Cultured HepG2 cells were seeded into Perfecta 3D® 96-well hanging drop plates and assessed over time for morphology, viability, cell cycle distribution, protein content and protein-mass profiles. Divergent aspects which were assessed included cell stocks, seeding density, volume of culture medium and use of extracellular matrix additives. Hanging drops are advantageous due to no complex culture matrix being present, enabling background free extractions for downstream experimentation. Varying characteristics were observed across cell stocks and batches, seeding density, culture medium volume and extracellular matrix when using immortalized HepG2 cells. These factors contribute to wide-ranging cellular responses and highlights concerns with respect to generating a reproducible phenotype in HepG2 hanging drop spheroids. Copyright © 2018 Elsevier Ltd. All rights reserved.

Adding the ‘heart’ to hanging drop networks for microphysiological multi-tissue experiments†

PubMed Central

Yazdi, Saeed Rismani; Shadmani, Amir; Bürgel, Sebastian C.; Misun, Patrick M.; Hierlemann, Andreas; Frey, Olivier

2017-01-01

Microfluidic hanging-drop networks enable culturing and analysis of 3D microtissue spheroids derived from different cell types under controlled perfusion and investigating inter-tissue communication in multi-tissue formats. In this paper we introduce a compact on-chip pumping approach for flow control in hanging-drop networks. The pump includes one pneumatic chamber located directly above one of the hanging drops and uses the surface tension at the liquid–air-interface for flow actuation. Control of the pneumatic protocol provides a wide range of unidirectional pulsatile and continuous flow profiles. With the proposed concept several independent hanging-drop networks can be operated in parallel with only one single pneumatic actuation line at high fidelity. Closed-loop medium circulation between different organ models for multi-tissue formats and multiple simultaneous assays in parallel are possible. Finally, we implemented a real-time feedback control-loop of the pump actuation based on the beating of a human iPS-derived cardiac microtissue cultured in the same system. This configuration allows for simulating physiological effects on the heart and their impact on flow circulation between the organ models on chip. PMID:26401602

Air Deployable Underwater Glider and Buoy Development for Arctic and Oceanographic Sensing

NASA Astrophysics Data System (ADS)

Legnos, P. J.

2013-12-01

LBI developed under a NOAA SBIR the AXIB (Airborne eXpendable Ice Buoy). The initial buoy was developed to collect barometric pressure, air temperature two meters above the surface and sea surface or ice temperature. A number of these AXIBs have been successfully deployed in the Arctic and Antarctic. Currently we are in the process of integrating additional sensors to include an anemometer, thermistor chain and hydrophones. Further development is in process for the integration of solar and wind recharging systems and lower power sensors and processing LBI developed under an ONR SBIR Grant two Air Deployable Underwater Gliders. They are primarily designed for air deployment from Navy P-3 or P-8 Aircraft though easily deployed from other aircraft or helicopters. The A-size (4 7/8'dia. X 36' long) and the 12 ¾ (12 ¾' dia. X 9' 9' long). On the development side we are in the process of integrating sensors and enhancing the battery storage capacity. We anticipate a broad range of Oceanographic sensing missions for these Gliders.

Marine mammal tracks from two-hydrophone acoustic recordings made with a glider

NASA Astrophysics Data System (ADS)

Küsel, Elizabeth T.; Munoz, Tessa; Siderius, Martin; Mellinger, David K.; Heimlich, Sara

2017-04-01

A multinational oceanographic and acoustic sea experiment was carried out in the summer of 2014 off the western coast of the island of Sardinia, Mediterranean Sea. During this experiment, an underwater glider fitted with two hydrophones was evaluated as a potential tool for marine mammal population density estimation studies. An acoustic recording system was also tested, comprising an inexpensive, off-the-shelf digital recorder installed inside the glider. Detection and classification of sounds produced by whales and dolphins, and sometimes tracking and localization, are inherent components of population density estimation from passive acoustics recordings. In this work we discuss the equipment used as well as analysis of the data obtained, including detection and estimation of bearing angles. A human analyst identified the presence of sperm whale (Physeter macrocephalus) regular clicks as well as dolphin clicks and whistles. Cross-correlating clicks recorded on both data channels allowed for the estimation of the direction (bearing) of clicks, and realization of animal tracks. Insights from this bearing tracking analysis can aid in population density estimation studies by providing further information (bearings), which can improve estimates.

Glider monitoring of shelf suspended particle dynamics and transport during storm and flooding conditions

NASA Astrophysics Data System (ADS)

Bourrin, François; Many, Gaël; Durrieu de Madron, Xavier; Martín, Jacobo; Puig, Pere; Houpert, Loic; Testor, Pierre; Kunesch, Stéphane; Mahiouz, Karim; Béguery, Laurent

2015-10-01

Transfers of particulate matter on continental margins primarily occur during energetic events. As part of the CASCADE (CAscading, Storm, Convection, Advection and Downwelling Events) experiment, a glider equipped with optical sensors was deployed in the coastal area of the Gulf of Lions, NW Mediterranean in March 2011 to assess the spatio-temporal variability of hydrology, suspended particles properties and fluxes during energetic conditions. This deployment complemented a larger observational effort, a part of the MOOSE (Mediterranean Ocean Observing System of the Environment) network, composed of a coastal benthic station, a surface buoy and moorings on the continental slope. This set of observations permitted to measure the impact of three consecutive storms and a flood event across the entire continental shelf. Glider data showed that the sediment resuspension and transport observed at the coastal station during the largest storm (Hs>4 m) was effective down to a water depth of 80 m. The mid-shelf mud belt, located between 40 and 90 m depth, appears as the zone where the along-shelf flux of suspended sediment is maximum. Besides, the across-shelf flux of suspended sediment converges towards the outer limit of the mid-shelf mud belt, where deposition of suspended particles probably occurs and contributes to the nourishment of this area. Hydrological structures, suspended particles transport and properties changed drastically during stormy periods and the following flood event. Prior to the storms, the shelf waters were weakly stratified due in particular to the presence of cold dense water on the inner- and mid-shelf. The storms rapidly swept away this dense water, as well as the resuspended sediments, along the shelf and towards a downstream submarine canyon. The buoyant river plumes that spread along the shelf after the flooding period provoked a restratification of the water column on the inner- and mid-shelf. The analysis of glider's optical data at

Development of a bespoke human factors taxonomy for gliding accident analysis and its revelations about highly inexperienced UK glider pilots.

PubMed

Jarvis, Steve; Harris, Don

2009-08-01

Low-hours solo glider pilots have a high risk of accidents compared to more experienced pilots. Numerous taxonomies for causal accident analysis have been produced for powered aviation but none of these is suitable for gliding, so a new taxonomy was required. A human factors taxonomy specifically for glider operations was developed and used to analyse all UK gliding accidents from 2002 to 2006 for their overall causes as well as factors specific to low hours pilots. Fifty-nine categories of pilot-related accident causation emerged, which were formed into progressively larger categories until four overall human factors groups were arrived at: 'judgement'; 'handling'; 'strategy'; 'attention'. 'Handling' accounted for a significantly higher proportion of injuries than other categories. Inexperienced pilots had considerably more accidents in all categories except 'strategy'. Approach control (path judgement, airbrake and speed handling) as well as landing flare misjudgement were chiefly responsible for the high accident rate in early solo glider pilots.

Evolution of the Marginal Ice Zone: Adaptive Sampling with Autonomous Gliders

DTIC Science & Technology

2015-09-30

kinetic energy (ε). Gliders also sampled dissolved oxygen, optical backscatter ( chlorophyll and CDOM fluorescence) and multi-spectral downwelling...Fig. 2). In the pack, Pacific Summer Water and a deep chlorophyll maximum form distinct layers at roughly 60 m and 80 m, respectively, which become...Sections across the ice edge just prior to recovery, during freeze-up, reveal elevated chlorophyll fluorescence throughout the mixed layer (Fig. 4

Prognosis at 6 and 12months after self-attempted hanging.

PubMed

Gantois, Guillaume; Parmentier-Decrucq, Erika; Duburcq, Thibault; Favory, Raphaël; Mathieu, Daniel; Poissy, Julien

2017-11-01

Patients surviving a self-attempted hanging have a total neurological recovery in 57-77% of cases at hospital discharge, but no long-term data are available. In this observational study, all patients hospitalized post-self-attempted hanging in the intensive care unit (ICU) in a 5-year period were included. Neurological evaluations at 6 and 12months were performed according to Cerebral Performance Category (CPC) scores. Factors associated with neurological recovery were determined by comparing CPC2+3+4 (bad recovery) vs. CPC1 (good recovery). Of 231 patients included, 104 (47%) were found to have cardiac arrest (CA). Ninety-five (41%) patients died in the ICU: 93 (89%) in the CA group and 2 (1.6%) in the group without CA. Neurological evaluations at 6 and 12months were obtained in 97 of the 136 surviving patients. At 6months, in the CA group (n=9), the CPC score was 1 for 6 patients, 2 for 2, and 4 for 1 patient. In the group without CA (n=88), 79 patients had normal neurological status at 6months and 78 at 12months. Among these patients, 96% returned home, 77% returned to work, 16 (18%) patients re-attempted suicide within the year. Risk factors of neurological sequelae at 6months were a CA at the hanging site (P=0.045), an elevated diastolic blood pressure (87 vs. 70 mm Hg; P=0.04), a lower initial Glasgow score (4 vs. 5; P=0.04), and an elevated blood glucose level (139 vs. 113 mg/dL; P<0.001). Patients surviving a self-attempted hanging who did not have a CA had a good neurological outcome. The rate of suicidal recidivism is particularly important, which justifies joint work with psychiatrists. Copyright © 2017 Elsevier Inc. All rights reserved.

Bloom Chasing With a Wave Glider: The MAGI (Mesoscale Features Aggregates Interaction) Project in the North Pacific

NASA Astrophysics Data System (ADS)

Wilson, C.; Villareal, T. A.; Anderson, E.

2015-12-01

Satellite ocean color data over the past decade has revealed the existence of large phytoplankton blooms in the North Pacific Ocean - specifically in the region NE of Hawai´I near 30°N. These blooms cover thousands of km2, persist for weeks or longer, and are often dominated by nitrogen-fixing diatom symbioses. These events have proven difficult to study outside of the time series station ALOHA at Hawai´i. The limited data indicates that the 30°N blooms are longer-lived, larger, and occur at a greater temperature range than the blooms that develop closer to Hawai´i. In the NE Pacific, at least some of these blooms occur at or near the subtropical front, a salinity-defined temperature compensated frontal zone that has a number of fronts imbedded in it. Here we will report on the results from the MAGI (Mesoscale features Aggregates Interaction) project. In this project, we deployed a Liquid Robotics SV2 Wave Glider® in June, 2015 for a multiple (up to 6) month mission to sample these features and assist in characterizing the bloom dynamics of this region. The Wave Gliders are the first unmanned autonomous marine robots to use only the ocean's wave energy for propulsion. The gliders are navigated remotely allowing a dynamic route through the keying of unique waypoints. Waypoints can be changed to sample features as they develop in the near-real time satellite imagery. The wave glider named Honey Badger is equipped with a CTD, two C3 fluorometers (one with an anti-biofouling coating applied), a Turner Designs PhytoFlash, meteorology and wave sensors, a downward facing camera, a Vengmar passive acoustic monitor, and a towed LISST-Holo.

Investigation of Stability and Control Characteristics of a 1/10-scale Model of a Canadian Tailless Glider in the Langley Free-flight Tunnel

NASA Technical Reports Server (NTRS)

Johnson, Joseph L.

1949-01-01

An investigation of the stability and control characteristics of a 1/10-scale model of a Canadian tailless glider has been conducted in the 10 Langley free-flight tunnel. The glider designated the N.R.L. tailless glider has a straight center section and outboard panels sweptback 43 deg. along the leading edge of the wing. The aspect ratio is 5.83 and the taper ratio is 0.323. From the results of the investigation and on the basis of comparison with higher-scale static tests of the National Research Council of Canada, it is expected that the longitudinal stability of the airplane will be satisfactory with flap up but unsatisfactory near the stall with flap down. The airplane is expected to have unsatisfactory lateral stability and control characteristics in the design configuration with either flap up or flap down. The model flights showed very low damping of the lateral oscillation. Increasing the vertical-tail area improved the lateral stability, and it appeared that a value of the directional-stability parameter C(sub n beta) of at least 0.002 per degree would probably be necessary for satisfactory lateral flying characteristics. A comparison of the calculated dynamic lateral stability characteristics of the N.R.L. tailless glider with those of a conventional-type sweptback airplane having a similar wing plan form and about the same inclination of the principal longitudinal axis of inertia showed that the tailless glider had poorer lateral stability because of the relatively larger radius of gyration in roll and the smaller damping-in-yaw factor C(sub nr).

Methods and results of boundary layer measurements on a glider

NASA Technical Reports Server (NTRS)

Nes, W. V.

1978-01-01

Boundary layer measurements were carried out on a glider under natural conditions. Two effects are investigated: the effect of inconstancy of the development of static pressure within the boundary layer and the effect of the negative pressure difference in a sublaminar boundary layer. The results obtained by means of an ion probe in parallel connection confirm those results obtained by means of a pressure probe. Additional effects which have occurred during these measurements are briefly dealt with.

Suicide attempt by hanging in preadolescent children: a case series.

PubMed

Omigbodun, O O; Adejumo, O A; Babalola, O O

2008-10-01

Suicide is now among the five top causes of death in youth worldwide. However, during the preadolescent period, suicidal behaviour is rare and difficult to define because the cognitive level of young children limits their ability to plan and understand the consequences or the finality of suicide. There is virtually no information about preadolescent suicidal behaviour in Nigeria. To illustrate the presentation and psychosocial issues associated with preadolescent suicidal attempt using the 'hanging' method in Nigeria. Three case scenarios of suicide attempt by hanging in preadolescents seen at the University College Hospital, Ibadan between 2005 and 2006 were interviewed in detail along with mental state and physical examination. Family and individual therapies were embarked upon. Types of psychopathology found in the preadolescents include depressive symptoms, conduct and oppositional defiant disorder and impulse control problems. Stressful life events such as family disruption, physical abuse, and bullying at school were factors associated with suicidal behaviour. The influence of the media in providing information about 'hanging' as a method of suicide was evident. Therapy yield varying results. High risk parameters for suicide in children should be known to all health professionals. The importance of intervention strategies particularly media education, monitoring systems and further research on suicidal behaviour in this environment is apparent.

A Rare Vehicle-Assisted Ligature Hanging: Suicide at the Wheel.

PubMed

Barranco, Rosario; Caputo, Fiorella; Bonsignore, Alessandro; Fraternali Orcioni, Giulio; Ventura, Francesco

2018-03-01

Suicide by hanging inside a motor vehicle is a rare occurrence. A 48-year-old woman suffering from major depression was found having agonal breathing inside her automobile. A 20-mm diameter blue nylon rope was wrapped tightly around her neck, with its other end tied to a nearby wooden fence post. Despite resuscitation attempts, she was declared deceased after several minutes. The vehicle was located in an area with a slight downward slope. The motor was off. It was in neutral gear, with the parking brake disengaged. Consequently, the gravitational forces, attributable to the mass of the vehicle and the declivity of the terrain, caused the rope to tighten.The dynamics fulfill the criteria for a partial hanging, given the difference in height between the point at which the rope was secured to the post and woman's neck, which in turn presented the typical oblique upward groove.This case, thus, represents a unique mode of partial hanging inside a passenger vehicle, rarely reported in the literature. The relative lack of internal injury is also noteworthy, along with the fact that the victim was discovered while still alive. The latter feature can be explained by the absence of the sudden or violent acceleration forces that can be generated with the engine on.

The alteration of extraocular muscle arc after hang-back recession in animal experiments.

PubMed

Lee, J; Kim, S

1996-01-01

We did an animal experimental study to investigate the extraocular muscle arc after hang-back recession on horizontal rectus muscles of five dogs. Two tiny sutures using 8-0 nylon were made on the sclera 8-10 mm posterior to the muscle insertion along the upper and lower margins of the right lateral rectus and left medial rectus to compare the altered muscle arc with the original muscle arc. Hang-back recession was performed on the horizontal rectus muscles and three months later we investigated the change in the muscle arc. Four of the 10 muscles operated showed no change, four were displaced upward (mean +/- SD; 1.00 +/- 0.16 mm) and two were displaced downward (1.00 +/- 0.00 mm). The average displacement was 0.60 +/- 0.52 mm. The alteration of muscle arc after hang-back recession thus seems insignificant.

Application of Density Estimation Methods to Datasets Collected From a Glider

DTIC Science & Technology

2015-09-30

species that occur in the locations where the acoustic data was recorded. For example, the data recorded off the west coast of Sardinia, Italy...fixed passive acoustic recordings to datasets recorded from an underwater glider. The current project will benefit from data collections with combined...Electromagnetics and Acoustics Research (NEAR) Laboratory was fitted with two High Tech, Inc., hydrophones (model # HTI-92-WB). The hydrophones were mounted one

Investigation of the Forces Acting on Gliders in Automobile-pulley-winch and Airplane Towed Flight

NASA Technical Reports Server (NTRS)

Klemperer, W B

1942-01-01

The magnitude, the direction, and the fluctuation of towing forces exerted upon gliders by towing them aloft behind an automobile, by means of a winch, and by airplane were measured under a variety of conditions covering a range from gentle to severe types of operation. For these tests the towing forces did not exceed 92 percent of the gross weight of the glider. The results indicate that in pulley and winch towing the towing forces are of about the same magnitude as in automobile towing. Speed increases in the accelerated phases of the towing jerks encountered in airplane towing can readily become critical as speeds in excess of placard speeds can be attained. Passage through the slipstream of the towing airplane can be equivalent to a severe gust that, at high speed, may impose high wing loads and require large control moments.

Common Cause Case Study: An Estimated Probability of Four Solid Rocket Booster Hold-down Post Stud Hang-ups

NASA Technical Reports Server (NTRS)

Cross, Robert

2005-01-01

Until Solid Rocket Motor ignition, the Space Shuttle is mated to the Mobil Launch Platform in part via eight (8) Solid Rocket Booster (SRB) hold-down bolts. The bolts are fractured using redundant pyrotechnics, and are designed to drop through a hold-down post on the Mobile Launch Platform before the Space Shuttle begins movement. The Space Shuttle program has experienced numerous failures where a bolt has "hung-up." That is, it did not clear the hold-down post before liftoff and was caught by the SRBs. This places an additional structural load on the vehicle that was not included in the original certification requirements. The Space Shuttle is currently being certified to withstand the loads induced by up to three (3) of eight (8) SRB hold-down post studs experiencing a "hang-up." The results af loads analyses performed for four (4) stud-hang ups indicate that the internal vehicle loads exceed current structural certification limits at several locations. To determine the risk to the vehicle from four (4) stud hang-ups, the likelihood of the scenario occurring must first be evaluated. Prior to the analysis discussed in this paper, the likelihood of occurrence had been estimated assuming that the stud hang-ups were completely independent events. That is, it was assumed that no common causes or factors existed between the individual stud hang-up events. A review of the data associated with the hang-up events, showed that a common factor (timing skew) was present. This paper summarizes a revised likelihood evaluation performed for the four (4) stud hang-ups case considering that there are common factors associated with the stud hang-ups. The results show that explicitly (i.e. not using standard common cause methodologies such as beta factor or Multiple Greek Letter modeling) taking into account the common factor of timing skew results in an increase in the estimated likelihood of four (4) stud hang-ups of an order of magnitude over the independent failure case.

Common Cause Case Study: An Estimated Probability of Four Solid Rocket Booster Hold-Down Post Stud Hang-ups

NASA Technical Reports Server (NTRS)

Cross, Robert

2005-01-01

Until Solid Rocket Motor ignition, the Space Shuttle is mated to the Mobil Launch Platform in part via eight (8) Solid Rocket Booster (SRB) hold-down bolts. The bolts are fractured using redundant pyrotechnics, and are designed to drop through a hold-down post on the Mobile Launch Platform before the Space Shuttle begins movement. The Space Shuttle program has experienced numerous failures where a bolt has hung up. That is, it did not clear the hold-down post before liftoff and was caught by the SRBs. This places an additional structural load on the vehicle that was not included in the original certification requirements. The Space Shuttle is currently being certified to withstand the loads induced by up to three (3) of eight (8) SRB hold-down experiencing a "hang-up". The results of loads analyses performed for (4) stud hang-ups indicate that the internal vehicle loads exceed current structural certification limits at several locations. To determine the risk to the vehicle from four (4) stud hang-ups, the likelihood of the scenario occurring must first be evaluated. Prior to the analysis discussed in this paper, the likelihood of occurrence had been estimated assuming that the stud hang-ups were completely independent events. That is, it was assumed that no common causes or factors existed between the individual stud hang-up events. A review of the data associated with the hang-up events, showed that a common factor (timing skew) was present. This paper summarizes a revised likelihood evaluation performed for the four (4) stud hang-ups case considering that there are common factors associated with the stud hang-ups. The results show that explicitly (i.e. not using standard common cause methodologies such as beta factor or Multiple Greek Letter modeling) taking into account the common factor of timing skew results in an increase in the estimated likelihood of four (4) stud hang-ups of an order of magnitude over the independent failure case.

75 FR 7936 - Airworthiness Directives; SCHEIBE-Flugzeugbau GmbH Model SF 25C Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-23

... Airworthiness Directives; SCHEIBE-Flugzeugbau GmbH Model SF 25C Gliders AGENCY: Federal Aviation Administration.... Relevant Service Information SCHEIBE-Flugzeugbau GmbH has issued SCHEIBE AIRCRAFT GMBH Service Bulletin 653... SCHEIBE-Flugzeugbau GmbH: Amendment 39-16208; Docket No. FAA-2010-0125; Directorate Identifier 2010-CE-005...

Anatomical basis of the liver hanging maneuver.

PubMed

Trotovsek, Blaz; Belghiti, Jacques; Gadzijev, Eldar M; Ravnik, Dean; Hribernik, Marija

2005-01-01

The anterior approach to right hepatectomy using the liver hanging maneuver without liver mobilization claims to be anatomically evaluated. During this procedure a 4 to 6-cm blind dissection between the inferior vena cava and the liver is performed. Short subhepatic veins, entering the inferior vena cava could be torn and a hemorrhage, difficult to control, could occur. On 100 corrosive casts of livers the anterior surface of the inferior vena cava was studied to evaluate the position, diameter and draining area of short subhepatic veins and inferior right hepatic vein. The width of the narrowest point on the planned route of blind dissection was determined. The average value of the narrowest point on the planned route of blind dissection was 8.7+/-2.3mm (range 2-15mm). The ideal angle of dissection being 0 degrees was found in 93% of cases. In 7% we found the angle of 5 degrees toward the right border of inferior vena cava to be the better choice. Our results show that liver hanging maneuver is a safe procedure. With the dissection in the proposed route the risk of disrupting short subhepatic veins is low (7%).

Resolving key drivers of variability through an important circulation choke point in the western Mediterranean Sea; using gliders, models & satellite remote sensing

NASA Astrophysics Data System (ADS)

Heslop, Emma; Aguiar, Eva; Mourre, Baptiste; Juza, Mélanie; Escudier, Romain; Tintoré, Joaquín

2017-04-01

The Ibiza Channel plays an important role in the circulation of the Western Mediterranean Sea, it governs the north/south exchange of different water masses that are known to affect regional ecosystems and is influenced by variability in the different drivers that affect sub-basins to the north (N) and south (S). A complex system. In this study we use a multi-platform approach to resolve the key drivers of this variability, and gain insight into the inter-connection between the N and S of the Western Mediterranean Sea through this choke point. The 6-year glider time series from the quasi-continuous glider endurance line monitoring of the Ibiza Channel, undertaken by SOCIB (Balearic Coastal Ocean observing and Forecasting System), is used as the base from which to identify key sub-seasonal to inter-annual patterns and shifts in water mass properties and transport volumes. The glider data indicates the following key components in the variability of the N/S flow of different water mass through the channel; regional winter mode water production, change in intermediate water mass properties, northward flows of a fresher water mass and the basin-scale circulation. To resolve the drivers of these components of variability, the strength of combining datasets from different sources, glider, modeling, altimetry and moorings, is harnessed. To the north atmospheric forcing in the Gulf of Lions is a dominant driver, while to the south the mesoscale circulation patterns of the Atlantic Jet and Alboran gyres dominate the variability but do not appear to influence the fresher inflows. Evidence of a connection between the northern and southern sub-basins is however indicated. The study highlights importance of sub-seasonal variability and the scale of rapid change possible in the Mediterranean, as well as the benefits of leveraging high resolution glider datasets within a multi-platform and modelling study.

37. PATTERNS HANGING FROM CEILING AND OFFICE WALL, NOTE CRAFTSMANSHIP ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

37. PATTERNS HANGING FROM CEILING AND OFFICE WALL, NOTE CRAFTSMANSHIP OF CURVE-LOOKING NORTHWEST. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

Simultaneous application of multiple platforms (Glider, Scanfish, profiling mooring, CTD) to improve detection and quantification of temporal ocean dynamics

NASA Astrophysics Data System (ADS)

Meyer, D.; Prien, R. D.; Lips, U.; Naumann, M.; Liblik, T.; Schulz-Bull, D. E.

2016-02-01

Ocean dynamics are difficult to observe given the broad spectrum of temporal and spatial scales. Robotic technology can be used to address this issue, and help to investigate the variability of physical and biogeochemical processes. This work focuses on ocean robots and in particular on glider technology which seems to be one of the most promising oceanographic tools for future marine research. In this context, we present the results of an observational program conducted in the Baltic Sea combining a profiling mooring (GODESS - Gotland Deep Environmental Sampling Station) and glider technology (Slocum). The temporal variability is captured by the mooring, while the spatial variability is obtained from the glider sampling the surrounding area. Furthermore, classical CTD-measurements and an underwater vehicle (Scanfish) are used simultaneously by two different research vessels to validate and complement the observing network. The main aim of the study is to identify possible synergies between the different platforms and to get a better understanding of maximizing the information content of the data collected by this network. The value and the quality of the data of each individual platform is analyzed and their contribution to the performance of the network itself evaluated.

Death by hanging while watching violent pornographic videos on the Internet - suicide or accidental autoerotic death?

PubMed

Vennemann, B; Pollak, S

2006-03-01

In deaths by hanging, it may sometimes be difficult to differentiate between autoerotic accident and suicide. Our report deals with a 30-year-old man who was found hanged in the living room of his flat. The deceased was wearing headphones connected to a PC. Within the deceased's view was a computer screen showing the last picture of a video file downloaded from the Internet with the head of an unclothed, allegedly hanged female. The deceased's left hand was inside his trousers in the genital region. The autopsy did not only show findings typical for hanging, but also advanced sarcoidosis, which was known to the victim. Although this basic illness could have been a possible motive for suicide, the circumstances in the presented case pointed more in the direction of an accidental autoerotic death. As far as we know, this is the first description of a death during autoerotic activity in which sexual stimulation was achieved by watching a video file downloaded from the Internet.

384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids.

PubMed

Hsiao, Amy Y; Tung, Yi-Chung; Qu, Xianggui; Patel, Lalit R; Pienta, Kenneth J; Takayama, Shuichi

2012-05-01

We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practical 3D cell-based high-throughput screening and enable broader use of the plate, we characterize the robustness of the 384 hanging drop array plate in terms of assay performance and demonstrate the versatility of the plate. We find that the 384 hanging drop array plate performance is robust in fluorescence- and colorimetric-based assays through Z-factor calculations. Finally, we demonstrate different plate capabilities and applications, including: spheroid transfer and retrieval for Janus spheroid formation, sequential addition of cells for concentric layer patterning of different cell types, and culture of a wide variety of cell types. Copyright © 2011 Wiley Periodicals, Inc.

384 Hanging Drop Arrays Give Excellent Z-factors and Allow Versatile Formation of Co-culture Spheroids

PubMed Central

Hsiao, Amy Y.; Tung, Yi-Chung; Qu, Xianggui; Patel, Lalit R.; Pienta, Kenneth J.; Takayama, Shuichi

2012-01-01

We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practical 3D cell-based high-throughput screening and enable broader use of the plate, we characterize the robustness of the 384 hanging drop array plate in terms of assay performance and demonstrate the versatility of the plate. We find that the 384 hanging drop array plate performance is robust in fluorescence- and colorimetric-based assays through z-factor calculations. Finally, we demonstrate different plate capabilities and applications, including: spheroid transfer and retrieval for Janus spheroid formation, sequential addition of cells for concentric layer patterning of different cell types, and culture of a wide variety of cell types. PMID:22161651

Private and Commercial Pilot: Glider. Flight Test Guide, Part 61 Revised, AC 61-61.

ERIC Educational Resources Information Center

Federal Aviation Administration (DOT), Washington, DC. Flight Standards Service.

This flight test guide assists the applicant and his instructor in preparing for the Private or Commercial Pilot Certificate with Glider Rating under Part 61 (revised) of Federal Aviation Regulations. It contains information and guidance concerning the pilot operations, procedures, and maneuvers relevant to the flight test required for that…

Evaluation of three popular diets fed to pet sugar gliders (Petaurus breviceps): Intake, digestion and nutrient balance.

PubMed

Dierenfeld, E S; Whitehouse-Tedd, K M

2018-02-01

Three diets fed to 12 pair-housed sugar gliders, Petaurus breviceps, were evaluated through 5-day intake and digestion trials following 10-day transitions. Diets 1 and 2 comprised liquid formula mixes with added vegetables and fruit, and Diet 3 comprised extruded pellets and a liquid formula. Diets eaten contained 16 -19% crude protein, 3%-15% crude fat, 10%-11% neutral detergent fibre, 4%-20% starch and 8%-49% sugar (dry basis). Calculated individual dry matter intakes (DMI) ranged from 3.9 to 5.1 g/day, representing 58.2-78.4 kJ/day. DMI was greater for Diet 2 (7.2% BW) vs. Diet 1 (5.6; p = .006) and Diet 3 (4.2% BW; p = .003). Although these differences were no longer detectable on a MBW basis, animals were shown to have gained BW (+14.2 g; p = .03) on Diet 2. In addition to nutrient composition differing widely among diets, DM digestibility (DMD) was higher in Diet 1 (91.2%) compared to Diet 2 (87.3%; p = .03), but DMD for Diet 3 (88.9%) did not differ from other diets. Gliders demonstrated ability to digest a variety of energy substrates, including simple sugars (96%-99%), fats (81%-96%) and starches (79%-98%), as well as substantial insoluble dietary fibre (58%-75%), with significant difference among diets demonstrated for some nutrients. Animals displayed selective feeding behaviours, rejecting insoluble fibre in produce and preferring the lipid-coated exterior of pellets. The diets used appeared to be balanced with respect to energy, protein and macromineral content, but may predispose to iron excess, other mineral imbalances (especially Ca deficiency) and obesity-clinical health issues described for pet gliders. Future focus on concentrations, types and utilization of dietary fibre in natural and captive diets, vitamin D metabolism and trace mineral interactions in sugar gliders would assist diet optimization for this highly gummivorous species. © 2017 Blackwell Verlag GmbH.

Modeling Primary Productivity in the Margin Ice Zone from Glider-Based Measurements of Chlorophyll and Light during the 2014 Miz Program

NASA Astrophysics Data System (ADS)

Perry, M. J.; Lee, C.; Rainville, L.; Cetinic, I.; Yang, E. J.; Kang, S. H.

2016-02-01

In late summer 2014 during the Marginal Ice Zone (MIZ) Experiment, an international project sponsored by ONR, four Seagliders transited open water, through the marginal ice zone, and under ice-covered regions in the Beaufort Sea, penetrating as far as 100 km into the ice pack. The gliders navigated either by GPS in open water or, when under the ice, by acoustics from sound sources embedded in the MIZ autonomous observing array. The glider sensor suite included temperature, temperature microstructure, salinity, oxygen, chlorophyll fluorescence, optical backscatter, and multi-spectral downwelling irradiance. Cruises on the IBRV Araon operating in the open Beaufort Sea and on the R/V Ukpik and Norseman operating in continental shelf waters off Alaska's north slope allowed us to construct proxy libraries for converting chlorophyll fluorescence to chlorophyll concentration and optical backscatter to particulate organic carbon concentration. Water samples were collected for chlorophyll and particulate organic carbon analysis on the cruises and aligned with optical profiles of fluorescence and backscatter using sensors that were factory calibrated at the same time as the glider sensors. Fields of chlorophyll, particulate organic carbon, light, and primary productivity are constructed from the glider data. Productivity is modeled as a function of chlorophyll and light, using photosynthesis-light (PE) models with available PE parameters from Arctic measurements. During August the region under the ice was characterized by a deep chlorophyll maximum layer with low rates of production in overlying waters. A phytoplankton bloom developed in open water at the end of September, preceding the rapid reformation of ice, despite shorter days and reduce irradiation.

Culturing muscle fibres in hanging drop: a novel approach to solve an old problem.

PubMed

Archacka, Karolina; Pozzobon, Michela; Repele, Andrea; Rossi, Carlo Alberto; Campanella, Michelangelo; De Coppi, Paolo

2014-02-01

The satellite cells (SCs) associated with muscle fibres play a key role in postnatal growth and regeneration of skeletal muscle. Commonly used methods of isolation and in vitro culture of SCs lead to the mixture of their subpopulations that exist within muscle. To solve this problem, we used the well established technique, the hanging drop system, to culture SCs in a three-dimensional environment and thus, to monitor them in their original niche. Using hanging drop technique, we were able to culture SCs associated with the fibre at least for 9 days with one transfer of fibres to the fresh drops. In comparison, in the classical method of myofibres culture, that is, on the dishes coated with Matrigel, SCs leave the fibres within 3 days after the isolation. Cells cultured in both systems differed in expression of Pax7 and MyoD. While almost all cells cultured in adhesion system expressed MyoD before the fifth day of the culture, the majority of SCs cultured in hanging drop still maintained expression of Pax7 and were not characterised by the presence of MyoD. Among the cells cultured with single myofibre for up to 9 days, we identified two different subclones of SCs: low proliferative clone and high proliferative clone, which differed in proliferation rate and membrane potential. The hanging drop enables the myofibres to be kept in suspension for at least 9 days, and thus, allows SCs and their niche to interact each other for prolonged time. In a consequence, SCs cultured in hanging drop maintain expression of Pax7 while those cultured in a traditional adhesion culture, that is, devoid of signals from the original niche, activate and preferentially undergo differentiation as manifested by expression of MyoD. Thus, the innovative method of SCs culturing in the hanging drop system may serve as a useful tool to study the fate of different subpopulations of these cells in their anatomical location and to determine reciprocal interactions between them and their niche. �

Christiaan Huygens and the Problem of the Hanging Chain

ERIC Educational Resources Information Center

Bukowski, John F.

2008-01-01

The seventeen-year-old Christiaan Huygens was the first to prove that a hanging chain did not take the form of the parabola, as was commonly thought in the early seventeenth century. We will examine Huygen's geometrical proof, and we will investigate the later history of the catenary.

Development of a bespoke human factors taxonomy for gliding accident analysis and its revelations about highly inexperienced UK glider pilots.

PubMed

Jarvis, Steve; Harris, Don

2010-02-01

Low-hours solo glider pilots have a high risk of accidents compared to more experienced pilots. Numerous taxonomies for causal accident analysis have been produced for powered aviation but none of these is suitable for gliding, so a new taxonomy was required. A human factors taxonomy specifically for glider operations was developed and used to analyse all UK gliding accidents from 2002 to 2006 for their overall causes as well as factors specific to low hours pilots. Fifty-nine categories of pilot-related accident causation emerged, which were formed into progressively larger categories until four overall human factors groups were arrived at: 'judgement'; 'handling'; 'strategy'; 'attention'. 'Handling' accounted for a significantly higher proportion of injuries than other categories. Inexperienced pilots had considerably more accidents in all categories except 'strategy'. Approach control (path judgement, airbrake and speed handling) as well as landing flare misjudgement were chiefly responsible for the high accident rate in early solo glider pilots. Statement of Relevance: This paper uses extant accident data to produce a taxonomy of underlying human factors causes to analyse gliding accidents and identify the specific causes associated with low hours pilots. From this specific, well-targeted remedial measures can be identified.

Studies on metatherian sex chromosomes. IX. Sex chromosomes of the greater glider (Marsupialia: Petauridae).

PubMed

Murray, J D; McKay, G M; Sharman, G B

1979-06-01

The greater glider, currently but incorrectly known as Schoinobates volans, is widely distributed in forested regions in eastern Australia. All animals studied from six different localities had 20 autosomes but there were four chromosomally distinct populations. At Royal National Park, N.S.W., all female greater gliders studied had 22 chromosomes including two large submetacentric X chromosomes with subterminal secondary constrictions in their longer arms. This form of X chromosome occurred also at Bondo State Forest, Myall Lakes and Coff's Harbour, N.S.W., and at Eidsvold, Qld. At Coomooboolaroo, Qld, the X chromosome was also a large submetacentric but a secondary constriction occurred in the shorter arm. Two chromosomally distinct types apparently occur in Royal National Park, one with XY males as in all other populations, and one with XY1Y2 males. Y or Y1, but not Y2, chromosomes were eliminated from the bone marrow in all populations but were present in spermatogonia, primary spermatocytes and cultured fibroblasts. Animals from Bondo State Forest had three or more acrocentric or metacentric supernumerary chromosomes.

75 FR 73074 - Duke Energy Hanging Rock II, LLC; Supplemental Notice That Initial Market-Based Rate Filing...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-29

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. ER11-2064-000] Duke Energy Hanging Rock II, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for... proceeding, of Duke Energy Hanging Rock II, LLC's application for market-based rate authority, with an...

Autonomous Gliders Observed Physical and Biogeochemical Interplay at Submesoscale during Deep Convection in the Gulf of Lions (NW Mediterranean)

NASA Astrophysics Data System (ADS)

Bosse, A.; Testor, P.; Damien, P.; D'Ortenzio, F.; Prieur, L. M.; Estournel, C.; Marsaleix, P.; Mortier, L.

2016-02-01

Since 2010, sustained observations of the circulation and water properties of the NW Mediterranean Sea have been carried out by gliders in the framework of the MOOSE observatory (Mediterranean Ocean Observatory System for the Environment: http://www.moose-network.fr/). They regularly sampled the wintertime Northern Current (NC), the deep convection zone as well as the North Balearic Front (NBF) collecting a great amount of physical and biogeochemical measurements.During periods of deep convection, the offshore mixed layer can reach great depths (>2300 m) in the Gulf of Lions. Baroclinic fronts then become very intense and reveal a lot of variability at submesoscale in the upper 500 m or so. In terms of process, symmetric instability has been evidenced to occurr during strong wind events by gliders measurements. Complementary analysis performed with the help of a high-resolution regional model (dx,dy=1 km) highlight the prominent role of downfront winds in triggering this instability. Important vertical exchanges of oceanic tracers at the front approximately aligned with isopycnals of magnitude O(100m/day) occur in response to this strong atmospheric forcing. Finally, gliders measurements of Chl-a fluorescence show how this frontal instability seems to stimulate phytoplankton growth in frontal regions during harsh wintertime conditions.

Hedgehogs and sugar gliders: respiratory anatomy, physiology, and disease.

PubMed

Johnson, Dan H

2011-05-01

This article discusses the respiratory anatomy, physiology, and disease of African pygmy hedgehogs (Atelerix albiventris) and sugar gliders (Petaurus breviceps), two species commonly seen in exotic animal practice. Where appropriate, information from closely related species is mentioned because cross-susceptibility is likely and because these additional species may also be encountered in practice. Other body systems and processes are discussed insofar as they relate to or affect respiratory function. Although some topics, such as special senses, hibernation, or vocalization, may seem out of place, in each case the information relates back to respiration in some important way. Copyright © 2011 Elsevier Inc. All rights reserved.

Evaluation of the mechanical behaviour of PathFile and ProGlider pathfinding nickel-titanium rotary instruments.

PubMed

Elnaghy, A M; Elsaka, S E

2015-09-01

To assess and compare the resistance to cyclic fatigue, torsional stress, bending and buckling of ProGlider (PG; Dentsply Maillefer, Ballaigues, Switzerland) instruments with PathFile (PF; Dentsply Maillefer) pathfinding nickel-titanium rotary instruments. Size 16, .02 taper PG and PF instruments were rotated in simulated canals until failure, and the number of cycles to failure (NCF) was recorded to evaluate their cyclic fatigue resistance. Torsional strength was measured using a torsiometer after fixing rigidly the apical 5 mm of the instrument. A scanning electron microscope was used to characterize the topographic features of the fracture surfaces of the instruments. The instruments were evaluated for bending resistance using a cantilever-bending test. The buckling resistance was measured by recording the maximum load required to form a lateral elastic displacement along the file axis using a universal testing machine. Data were statistically analysed using independent t-tests. Statistical significance was set at P < 0.05. ProGlider instrument had a significantly higher flexibility, higher resistance to cyclic fatigue and torsional stress than PF instruments (P < 0.05). The fractured cross-sectional surfaces revealed typical features of cyclic fatigue and torsional fractures. There was no significant difference in the maximum load needed to buckle the two instruments tested (P = 0.082). ProGlider NiTi pathfinding instrument manufactured from M-Wire alloy had enhanced mechanical properties, including higher flexibility, higher resistance to cyclic fatigue and torsional stress compared with PathFile instrument made of conventional NiTi alloy. © 2014 International Endodontic Journal. Published by John Wiley & Sons Ltd.

An optimized protocol for handling and processing fragile acini cultured with the hanging drop technique.

PubMed

Snyman, Celia; Elliott, Edith

2011-12-15

The hanging drop three-dimensional culture technique allows cultivation of functional three-dimensional mammary constructs without exogenous extracellular matrix. The fragile acini are, however, difficult to preserve during processing steps for advanced microscopic investigation. We describe adaptations to the protocol for handling of hanging drop cultures to include investigation using confocal, scanning, and electron microscopy, with minimal loss of cell culture components. Copyright © 2011 Elsevier Inc. All rights reserved.

Sea surface cooling in the Northern South China Sea observed using Chinese Sea-wing Underwater Glider measurements

NASA Astrophysics Data System (ADS)

Qiu, C.; Mao, H.; Wu, J.

2016-02-01

Based on 26 days of Chinese Seawing underwater Glider measurements and satellite microwave data, we documented cooling of the upper mixed layer of the ocean in response to changes in the wind in the Northern South China Sea (NSCS) from September 19, 2014, to October 15, 2014. The Seawing underwater glider measured 177 profiles of temperature, salinity, and pressure within a 55 km נ55 km area, and reached a depth of 1000 m at a temporal resolution of 4 h. The study area experienced two cooling events, Cooling I and Cooling II, according to their timing. During Cooling I, water temperature at 1m depth (T1) decreased by 1.0°C, and the corresponding satellitederived surface winds increased locally by 4.2 m/s. During Cooling II, T1 decreased sharply by 1.7°C within a period of 4 days; sea surface winds increased by 7 m/s and covered the entire NSCS. The corresponding mixed layer depth (MLD) deepened sharply from 30 m to 60 m during Cooling II, and remained steady during Cooling I. We estimated temperature tendencies using a ML model. High resolution Seawing underwater glider measurements provided an estimation of MLD migration, allowing us to obtain the temporal entrainment rate of cool sub thermocline water. Quantitative analysis confirmed that the entrainment rate and latent heat flux were the two major components that regulated cooling of the ML, and that the Ekman advection and sensible heat flux were small.

Diagonal gaits in the feathertail glider Acrobates pygmaeus (Acrobatidae, Diprotodontia): Insights for the evolution of primate quadrupedalism.

PubMed

Karantanis, Nikolaos-Evangelos; Youlatos, Dionisios; Rychlik, Leszek

2015-09-01

Research on primate origins has revolved around arboreality and, more specifically, the adaptations that are linked to safe navigation in the fine-branch niche. To this end, extant non-primate mammals have been used as models to assess the significance of these adaptations. However, the size of these models is larger than that estimated for early primates. In contrast, the feathertail marsupial glider Acrobates pygmaeus, with a body mass of 12 g, a clawless opposable hallux, and terminal branch feeding habits appears more suited to modeling behavioral adaptations to the small branch milieu. Analysis of video recordings of 18 feathertail gliders walking on poles of variable diameter and inclination revealed that they preferentially used diagonal sequence gaits, fast velocities and low duty factors. Diagonal gaits did not correlate to duty factor, but increased as substrate size decreased, and from descending to ascending locomotion. Furthermore, the duty factor index increased in more diagonal gaits and ascending locomotion. Finally, velocities were lower on smaller substrates, and were mainly regulated by stride frequency and, to a lesser degree, stride length. Feathertail glider gaits displayed noteworthy behavioral convergences with primate quadrupedalism, but some of these results need additional investigation. Despite any discrepancies, these features appear to be favorable for quadrupedal progression on small branches, providing a selective advantage for navigating within a fine branch niche and highlighting the importance of small body size in early primate evolution. Copyright © 2015 Elsevier Ltd. All rights reserved.

The impact of underwater glider observations in the forecast of Hurricane Gonzalo (2014)

NASA Astrophysics Data System (ADS)

Goni, G. J.; Domingues, R. M.; Kim, H. S.; Domingues, R. M.; Halliwell, G. R., Jr.; Bringas, F.; Morell, J. M.; Pomales, L.; Baltes, R.

2017-12-01

The tropical Atlantic basin is one of seven global regions where tropical cyclones (TC) are commonly observed to originate and intensify from June to November. On average, approximately 12 TCs travel through the region every year, frequently affecting coastal, and highly populated areas. In an average year, 2 to 3 of them are categorized as intense hurricanes. Given the appropriate atmospheric conditions, TC intensification has been linked to ocean conditions, such as increased ocean heat content and enhanced salinity stratification near the surface. While errors in hurricane track forecasts have been reduced during the last years, errors in intensity forecasts remain mostly unchanged. Several studies have indicated that the use of in situ observations has the potential to improve the representation of the ocean to correctly initialize coupled hurricane intensity forecast models. However, a sustained in situ ocean observing system in the tropical North Atlantic Ocean and Caribbean Sea dedicated to measuring subsurface thermal and salinity fields in support of TC intensity studies and forecasts has yet to be implemented. Autonomous technologies offer new and cost-effective opportunities to accomplish this objective. We highlight here a partnership effort that utilize underwater gliders to better understand air-sea processes during high wind events, and are particularly geared towards improving hurricane intensity forecasts. Results are presented for Hurricane Gonzalo (2014), where glider observations obtained in the tropical Atlantic: Helped to provide an accurate description of the upper ocean conditions, that included the presence of a low salinity barrier layer; Allowed a detailed analysis of the upper ocean response to hurricane force winds of Gonzalo; Improved the initialization of the ocean in a coupled ocean-atmosphere numerical model; and together with observations from other ocean observing platforms, substantially reduced the error in intensity forecast

Water resources and effects of potential surface coal mining on dissolved solids in Hanging Woman Creek basin, southeastern Montana

USGS Publications Warehouse

Cannon, M.R.

1989-01-01

Groundwater resources of the Hanging Woman Creek basin, Montana include Holocene and Pleistocene alluvial aquifers and sandstone , coal, and clinker aquifers in the Paleocene Fort Union Formation. Surface water resources are composed of Hanging Woman Creek, its tributaries, and small stock ponds. Dissolved-solids concentrations in groundwater ranged from 200 to 11,00 mg/L. Generally, concentrations were largest in alluvial aquifers and smallest in clinker aquifers. Near its mouth, Hanging Woman Creek had a median concentration of about 1,800 mg/L. Mining of the 20-foot to 35-foot-thick Anderson coal bed and 3-foot to 16-foot thick Dietz coal bed could increase dissolved-solids concentrations in shallow aquifers and in Hanging Woman Creek because of leaching of soluble minerals from mine spoils. Analysis of saturated-paste extracts from 158 overburden samples indicated that water moving through mine spoils would have a median increase in dissolved-solids concentration of about 3,700 mg/L, resulting in an additional dissolved-solids load to Hanging Woman Creek of about 3.0 tons/day. Hanging Woman Creek near Birney could have an annual post-mining dissolved-solids load of 3,415 tons at median discharge, a 47% increase from pre-mining conditions load. Post-mining concentrations of dissolved solids, at median discharge, could range from 2,380 mg/L in March to 3,940 mg/L in August, compared to mean pre-mining concentrations that ranged from 1,700 mg/L in July, November, and December to 2,060 mg/L in May. Post-mining concentrations and loads in Hanging Woman Creek would be smaller if a smaller area were mined. (USGS)

White River National Forest Hanging Lake transportation and operations study

DOT National Transportation Integrated Search

2017-05-01

Hanging Lake is a recreation site located on land managed by the U.S. Forest Service (USFS) under the jurisdiction of the White River National Forests Eagle-Holy Cross Ranger District. Due to its increasing popularity over the past few years, the ...

Detail view to show the bronze gates hanging in the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail view to show the bronze gates hanging in the driveway portals; the open grille is foliated and crowned with patriotic eagle emblems - United States Department of Commerce, Bounded by Fourteenth, Fifteenth, and E streets and Constitution Avenue, Washington, District of Columbia, DC

Unusual planned complex suicide committed with a muzzle-loading pistol in combination with subsequent hanging.

PubMed

Ondruschka, Benjamin; Morgenthal, Sylvia; Dreβler, Jan; Bayer, Ronny

2016-11-01

In Germany, suicides by firearms are not very common in contrast to deaths by hanging and intoxications. The use of historical muzzle-loading firearms in the context of suicides is a rarity. Contact shots from muzzle loaders cause an unusual wound morphology with extensive soot soiling. We report the case of a 59-year-old man, who committed a planned complex suicide by shooting into his mouth with a replica percussion gun in combination with hanging. The gunshot injury showed strong explosive effects in the oral cavity with fractures of the facial bones and the skull associated with cerebral evisceration (so-called Krönlein shot). Due to the special constellation of the case with hanging immediately after the shot, external bleeding from the head injuries was only moderate. Therefore, the head injuries could be assessed and partially reconstructed already at the scene.

A tree branch instead of a ligature: an unusual accidental hanging.

PubMed

Vadysinghe, Amal Nishantha; Sivasubramanium, Murugupillai; Jayasooriya, Rankothge Pemasiri

2017-12-01

A unique case of accidental hanging due to compression of the neck of an adult by the branches of a coffee tree is reported. The decedent was a 42-year-old male who was found dead in a semi prone position on a slope. His neck was lodged in a wedge formed by two branches of a coffee tree, with his legs angled downwards on the slope. Autopsy revealed two friction abrasions located horizontally on either side of the front of the neck, just above the larynx. The findings were compatible with compression of the neck by the branches of the tree, with the body weight of the decedent contributing to compression. Subsequent complete autopsy examination confirmed the cause of death as hanging. Following an inquest the death was ruled to be accidental.

High-frequency internal waves and thick bottom mixed layers observed by gliders in the Gulf Stream

NASA Astrophysics Data System (ADS)

Todd, Robert E.

2017-06-01

Autonomous underwater gliders are conducting high-resolution surveys within the Gulf Stream along the U.S. East Coast. Glider surveys reveal two mechanisms by which energy is extracted from the Gulf Stream as it flows over the Blake Plateau, a portion of the outer continental shelf between Florida and North Carolina where bottom depths are less than 1000 m. Internal waves with vertical velocities exceeding 0.1 m s-1 and frequencies just below the local buoyancy frequency are routinely found over the Blake Plateau, particularly near the Charleston Bump, a prominent topographic feature. These waves are likely internal lee waves generated by the subinertial Gulf Stream flow over the irregular bathymetry of the outer continental shelf. Bottom mixed layers with O(100) m thickness are also frequently encountered; these thick bottom mixed layers likely form in the lee of topography due to enhanced turbulence generated by O(1) m s-1 near-bottom flows.

Application of hanging drop technique for stem cell differentiation and cytotoxicity studies.

PubMed

Banerjee, Meenal; Bhonde, Ramesh R

2006-05-01

The aim of our study is to explore the possibility of using an ancient method of culture technique- the hanging drop technique for stem cell differentiation and cytotoxicity testing. We demonstrate here a variety of novel applications of this age old technique not only to harness the differentiation potential of stem cells into specific lineages but also for cytotoxicity studies. Here we have prepared hanging drop cultures by placing 20 microl micro-drops of nutrient media and 10% Fetal Calf Serum (FCS) containing cells of interest on the lids of 60 mm dishes. Bottom plates of the dishes were filled with sterile Phosphate Buffer Saline (PBS) to avoid desiccation of samples. Lids were then placed on the bottom plates to achieve hanging drop cultures. We utilized this technique for cultivation of ciliated epithelia to study cytotoxicity and differentiation of bone marrow stromal cells. Most importantly the modified culture technique presented here is simple, economical and cost effective in terms of the time taken and the reagents required and are amenable to goal specific modification such as cytotoxicity testing. It is advantageous over the existing system in terms of retention of viability and functionality for longer duration and for providing three dimensional growth micro-environment making it useful for organotypic cultures and in vivo simulation.

Monitoring water masses properties by Glider in Sardinia Channel during summer 2014

NASA Astrophysics Data System (ADS)

Gana, Slim; Iudicone, Daniele; Ghenim, Leila; Mortier, Laurent; Testor, Pierre; Tintoré, Joaquin; Olita, Antonio

2015-04-01

1. Summary In the framework of the EC funded project, PERSEUS (WP3, Subtask 3.3.1: Repeated glider sections in key channels and sub-basin) and with the support of JERICO TNA (EU-FP7), a deep water glider (up to 1000m) was deployed from the R/V Tethys in the Sardinia Channel and has carried out 3 return trips during the period spanning from the 16th of August 2014 to the 19th of September 2014. The Gilder was equipped with CTD, O2 sensors, Fluorometers (ChlA), back scattering from 470 to 880 nm and was programmed to follow a path close to SARAL satellite track #887. During this experiment, a significant dataset, as never obtained before for this area, has been collected. The innovation stands in the high spatial resolution, in the temporal repetitivity and in the number of parameters sampled simultaneously. The first step of the work will focuses on the analysis of the hydrological properties of the existing water masses in the area. 2. Frame and aim of the experiment The Sardinia Channel is a zonally oriented passage connecting the Algerian and the Tyrrhenian basins, with a sill depth of about 1900 m. In spite of the considerable amount of work achieved and accurate results obtained about the circulation in the Western Mediterranean Sea, during the last 20 years, the Sardinia Channel is still one of the region where the dynamical processes and water exchanges are not clearly identified. Previous studies (Garzoli S. and C. Maillard, 1979, and Ozturgut Erdogan, 1975) pointed out the complexity of the processes in the region and the role of the bottom topography in sustaining them, and provided a first estimation of the involved fluxes. The main knowledge about the water masses crossing this region mostly concerns the AW (Atlantic Water) and the LIW (Levantine Intermediate Water). Along the Algerian coast, the AW is transported mainly by the Algerian current (AC Millot, 1985) from which the anticyclonic Algerian eddies (AEs, Puillat et al., 2002; Taupier-Letage et al

Digestion and absorption ofEucalyptus essential oils in greater glider (Petauroide svolans) and brushtail possum (Trichosurus vulpecula).

PubMed

Foley, W J; Lassak, E V; Brophy, J

1987-11-01

Measurements were made of the quantity and composition of the steam-volatile essential oils in gastrointestinal tract contents of greater gliders fedEucalyptus radiata foliage and brushtail possums fedE. melliodora foliage. In both species, there was less oil in the stomach contents than in an equivalent mass of foliage. Only minor losses of leaf oils occurred during mastication by greater gliders, and absorption from the stomach appeared to be the major reason for the difference in the oil content of ingested leaves and of stomach contents. The apparent digestibility of oils over the whole gut was 96-97 %, although oils from the cecum and feces of both species contained compounds not present in the original leaf oils. Absorption of oils before they reach the hindgut should reduce the severity of antimicrobial effects but may involve a metabolic cost to the animal in detoxification and excretion.

Hematological and plasma biochemical values of the greater glider in Australia.

PubMed

Viggers, K L; Lindenmayer, D B

2001-04-01

Reference hematological and plasma biochemical values are presented for the greater glider (Petauroides volans) at Tumut (southeastern New South Wales, Australia). Nineteen animals were sampled during a capture period of 1 wk in August 1999. Values for red cell counts were significantly higher in male animals (mean +/- SE; males: 5.6 +/- 0.1; females: 5.2 +/- 0.1). Young animals had higher white cell counts than older ones (mean +/- SE; young: 4.9 +/- 0.4; older: 2.8 +/- 0.4). Lymphocytes were the predominant white blood cell type in this species.

The formation of intestinal organoids in a hanging drop culture.

PubMed

Panek, Malgorzata; Grabacka, Maja; Pierzchalska, Malgorzata

2018-01-25

Recently organoids have become widely used in vitro models of many tissue and organs. These type of structures, originated from embryonic or adult mammalian intestines, are called "mini guts". They organize spontaneously when intestinal crypts or stem cells are embedded in the extracellular matrix proteins preparation scaffold (Matrigel). This approach has some disadvantages, as Matrigel is undefined (the concentrations of growth factors and other biologically active components in it may vary from batch to batch), difficult to handle and expensive. Here we show that the organoids derived from chicken embryo intestine are formed in a hanging drop without embedding, providing an attractive alternative for currently used protocols. Using this technique we obtained compact structures composed of contiguous organoids, which were generally similar to chicken organoids cultured in Matrigel in terms of morphology and expression of intestinal epithelial markers. Due to the simplicity, high reproducibility and throughput capacity of hanging drop technique our model may be applied in various studies concerning the gut biology.

Sea surface cooling in the Northern South China Sea observed using Chinese sea-wing underwater glider measurements

NASA Astrophysics Data System (ADS)

Qiu, Chunhua; Mao, Huabin; Yu, Jiancheng; Xie, Qiang; Wu, Jiaxue; Lian, Shumin; Liu, Qinyan

2015-11-01

Based on 26 days of Chinese Sea-wing underwater glider measurements and satellite microwave data, we documented cooling of the upper mixed layer of the ocean in response to changes in the wind in the Northern South China Sea (NSCS) from September 19, 2014, to October 15, 2014. The Sea-wing underwater glider measured 177 profiles of temperature, salinity, and pressure within a 55 km×55 km area, and reached a depth of 1000 m at a temporal resolution of ∼4 h. The study area experienced two cooling events, Cooling I and Cooling II, according to their timing. During Cooling I, water temperature at 1-m depth (T1) decreased by ∼1.0 °C, and the corresponding satellite-derived surface winds increased locally by 4.2 m/s. During Cooling II, T1 decreased sharply by 1.7 °C within a period of 4 days; sea surface winds increased by 7 m/s and covered the entire NSCS. The corresponding mixed layer depth (MLD) deepened sharply from 30 m to 60 m during Cooling II, and remained steady during Cooling I. We estimated temperature tendencies using a ML model. High resolution Sea-wing underwater glider measurements provided an estimation of MLD migration, allowing us to obtain the temporal entrainment rate of cool sub-thermocline water. Quantitative analysis confirmed that the entrainment rate and latent heat flux were the two major components that regulated cooling of the ML, and that the Ekman advection and sensible heat flux were small.

Flat-ramp vs. convex-concave thrust geometries in a deformable hanging wall: new insights from analogue modeling experiments

NASA Astrophysics Data System (ADS)

Almeida, Pedro; Tomas, Ricardo; Rosas, Filipe; Duarte, Joao; Terrinha, Pedro

2015-04-01

Different modes of strain accommodation affecting a deformable hanging-wall in a flat-ramp-flat thrust system were previously addressed through several (sandbox) analog modeling studies, focusing on the influence of different variables, such as: a) thrust ramp dip angle and friction (Bonini et al, 2000); b) prescribed thickness of the hanging-wall (Koy and Maillot, 2007); and c) sin-thrust erosion (compensating for topographic thrust edification, e.g. Persson and Sokoutis, 2002). In the present work we reproduce the same experimental procedure to investigate the influence of two different parameters on hanging-wall deformation: 1) the geometry of the thrusting surface; and 2) the absence of a velocity discontinuity (VD) that is always present in previous similar analogue modeling studies. Considering the first variable we use two end member ramp geometries, flat-ramp-flat and convex-concave, to understand the control exerted by the abrupt ramp edges in the hanging-wall stress-strain distribution, comparing the obtain results with the situation in which such edge singularities are absent (convex-concave thrust ramp). Considering the second investigated parameter, our motivation was the recognition that the VD found in the different analogue modeling settings simply does not exist in nature, despite the fact that it has a major influence on strain accommodation in the deformable hanging-wall. We thus eliminate such apparatus artifact from our models and compare the obtained results with the previous ones. Our preliminary results suggest that both investigated variables play a non-negligible role on the structural style characterizing the hanging-wall deformation of convergent tectonic settings were such thrust-ramp systems were recognized. Acknowledgments This work was sponsored by the Fundação para a Ciência e a Tecnologia (FCT) through project MODELINK EXPL/GEO-GEO/0714/2013. Pedro Almeida wants to thank to FCT for the Ph.D. grant (SFRH/BD/52556/2014) under the

75 FR 43103 - Airworthiness Directives; CENTRAIR Models 101, 101A, 101P, and 101AP Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-23

... tube and, if necessary, replacing it. You may obtain further information by examining the MCAI in the... the rudder bar locking adjustment tube of a non- reinforced version have been reported to Soci[eacute]t[eacute] Nouvelle (SN) Centrair. This tube had been reinforced in 1984 with a modification. Gliders...

Rock Around the Clock. Hanging Rock State Park: An Environmental Education Learning Experience Designed for Grade 5.

ERIC Educational Resources Information Center

Dow, Jaye

This learning packet, one in a series of eight, was developed by the Hanging Roek State Park in North Carolina for grade 5 to teach about the geology of the park. Loose-leaf pages are presented in nine sections that contain: (1) introductions to the North Carolina State Park System, the Hanging Rock State Park, the park's activity packet, and to…

Spray and dust hang in the air, as the demolition ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Spray and dust hang in the air, as the demolition is completed. Only the southeast pier remains standing at right. View is to southeast from confluence of Trinity and South Fork Trinity Rivers - South Fork Trinity River Bridge, State Highway 299 spanning South Fork Trinity River, Salyer, Trinity County, CA

Evaluation of a universal long-lasting insecticidal net (LLIN) distribution campaign in Ghana: cost effectiveness of distribution and hang-up activities

PubMed Central

2014-01-01

Background Between May 2010 and October 2012, approximately 12.5 million long-lasting insecticidal nets (LLINs) were distributed through a national universal mass distribution campaign in Ghana. The campaign included pre-registration of persons and sleeping places, door-to-door distribution of LLINs with ‘hang-up’ activities by volunteers and post-distribution ‘keep-up’ behaviour change communication activities. Hang-up activities were included to encourage high and sustained use. Methods The cost and cost-effectiveness of the LLIN Campaign were evaluated using a before-after design in three regions: Brong Ahafo, Central and Western. The incremental cost effectiveness of the ‘hang-up’ component was estimated using reported variation in the implementation of hang-up activities and LLIN use. Economic costs were estimated from a societal perspective assuming LLINs would be replaced after three years, and included the time of unpaid volunteers and household contributions given to volunteers. Results Across the three regions, 3.6 million campaign LLINs were distributed, and 45.5% of households reported the LLINs received were hung-up by a volunteer. The financial cost of the campaign was USD 6.51 per LLIN delivered. The average annual economic cost was USD 2.90 per LLIN delivered and USD 6,619 per additional child death averted by the campaign. The cost-effectiveness of the campaign was sensitive to the price, lifespan and protective efficacy of LLINs. Hang-up activities constituted 7% of the annual economic cost, though the additional financial cost was modest given the use of volunteers. LLIN use was greater in households in which one or more campaign LLINs were hung by a volunteer (OR = 1.57; 95% CI = 1.09, 2.27; p = 0.02). The additional economic cost of the hang-up activities was USD 0.23 per LLIN delivered, and achieved a net saving per LLIN used and per death averted. Conclusion In this campaign, hang-up activities were estimated to be net

Upper ocean response to Hurricane Gonzalo (2014): Salinity effects revealed by targeted and sustained underwater glider observations

NASA Astrophysics Data System (ADS)

Domingues, Ricardo; Goni, Gustavo; Bringas, Francis; Lee, Sang-Ki; Kim, Hyun-Sook; Halliwell, George; Dong, Jili; Morell, Julio; Pomales, Luis

2015-09-01

During October 2014, Hurricane Gonzalo traveled within 85 km from the location of an underwater glider situated north of Puerto Rico. Observations collected before, during, and after the passage of this hurricane were analyzed to improve our understanding of the upper ocean response to hurricane winds. The main finding in this study is that salinity potentially played an important role on changes observed in the upper ocean; a near-surface barrier layer likely suppressed the hurricane-induced upper ocean cooling, leading to smaller than expected temperature changes. Poststorm observations also revealed a partial recovery of the ocean to prestorm conditions 11 days after the hurricane. Comparison with a coupled ocean-atmosphere hurricane model indicates that model-observations discrepancies are largely linked to salinity effects described. Results presented in this study emphasize the value of underwater glider observations for improving our knowledge of how the ocean responds to tropical cyclone winds and for tropical cyclone intensification studies and forecasts.

AMT-200S Motor Glider Parameter and Performance Estimation

NASA Technical Reports Server (NTRS)

Taylor, Brian R.

2011-01-01

Parameter and performance estimation of an instrumented motor glider was conducted at the National Aeronautics and Space Administration Dryden Flight Research Center in order to provide the necessary information to create a simulation of the aircraft. An output-error technique was employed to generate estimates from doublet maneuvers, and performance estimates were compared with results from a well-known flight-test evaluation of the aircraft in order to provide a complete set of data. Aircraft specifications are given along with information concerning instrumentation, flight-test maneuvers flown, and the output-error technique. Discussion of Cramer-Rao bounds based on both white noise and colored noise assumptions is given. Results include aerodynamic parameter and performance estimates for a range of angles of attack.

Neurological Outcomes Following Suicidal Hanging: A Prospective Study of 101 Patients

PubMed Central

Jawaid, Mohammed Turab; Amalnath, S. Deepak; Subrahmanyam, D. K. S.

2017-01-01

Context: Survivors of suicidal hanging can have variable neurological outcomes – from complete recovery to irreversible brain damage. Literature on the neurological outcomes in these patients is confined to retrospective studies and case series. Hence, this prospective study was carried out. Aims: The aim is to study the neurological outcomes in suicidal hanging. Settings and Design: This was a prospective observational study carried out from July 2014 to July 2016. Subjects and Methods: Consecutive patients admitted to the emergency and medicine wards were included in the study. Details of the clinical and radiological findings, course in hospital and at 1 month postdischarge were analyzed. Statistical Analysis Used: Statistical analysis was performed using IBM SPSS advanced statistics 20.0 (SPSS Inc., Chicago, USA). Univariate analysis was performed using Chi-square test for significance and Odd's ratio was calculated. Results: Of the 101 patients, 6 died and 4 had residual neuro deficits. Cervical spine injury was seen in 3 patients. Interestingly, 39 patients could not remember the act of hanging (retrograde amnesia). Hypotension, pulmonary edema, Glasgow coma scale (GCS) score <8 at admission, need for mechanical ventilation, and cerebral edema on plain computed tomography were more in those with amnesia as compared to those with normal memory and these findings were statistically significant. Conclusions: Majority of patients recovered without any sequelae. Routine imaging of cervical spine may not be warranted in all patients, even in those with poor GCS. Retrograde amnesia might be more common than previously believed and further studies are needed to analyze this peculiar feature. PMID:28584409

In vitro differentiation of mouse embryonic stem (mES) cells using the hanging drop method.

PubMed

Wang, Xiang; Yang, Phillip

2008-07-23

Stem cells have the remarkable potential to develop into many different cell types. When a stem cell divides, each new cell has the potential to either remain a stem cell or become another type of cell with a more specialized function, This promising of science is leading scientists to investigate the possibility of cell-based therapies to treat disease. When culture in suspension without antidifferentiation factors, embryonic stem cells spontaneously differentiate and form three-dimensional multicellular aggregates. These cell aggregates are called embryoid bodies(EB). Hanging drop culture is a widely used EB formation induction method. The rounded bottom of hanging drop allows the aggregation of ES cells which can provide mES cells a good environment for forming EBs. The number of ES cells aggregatied in a hanging drop can be controlled by varying the number of cells in the initial cell suspension to be hung as a drop from the lid of Petri dish. Using this method we can reproducibly form homogeneous EBs from a predetermined number of ES cells.

Three-dimensional culture of buffalo granulosa cells in hanging drop mimics the preovulatory follicle stage.

PubMed

Yadav, Monica; Agrawal, Himanshu; Pandey, Mamta; Singh, Dheer; Onteru, Suneel K

2018-03-01

Granulosa cell (GC) culture models mimicking the intrafollicular environment are limited. Such models have a great potential in reproductive toxicity studies. The buffalo, a monovulatory species like humans, could be a better model than polyovulatory rodents. Therefore, we targeted the development and characterization of three-dimensional (3D) culture systems for buffalo GCs. The GCs from small ovarian follicles (SF) maintained the CYP19 gene expression for 144 hr in a 2D culture system. Hence, GCs from SF were cultured directly in 3D using hanging drop and Poly-([2-hydroxyethyl methacrylate]) (polyHEMA) methods in the DMEM media containing 1 ng/ml FSH and 10 ng/ml IGF-1 for 144 hr. The expression profile of nine GC-specific transcripts; CYP19, TNFAIP6, AMH, PTI, NR4A1, FSHR, RUNX, LHR, and COX2/PTGS2; revealed that 3D-spheroids developed in hanging drop method maintained the GC phenotype of preovulatory follicles. Therefore, hanging drop method is a best method for culturing GCs to mimic the intrafollicular environment. © 2017 Wiley Periodicals, Inc.

Controlling Vapor Pressure In Hanging-Drop Crystallization

NASA Technical Reports Server (NTRS)

Carter, Daniel C.; Smith, Robbie

1988-01-01

Rate of evaporation adjusted to produce larger crystals. Device helps to control vapor pressure of water and other solvents in vicinity of hanging drop of solution containing dissolved enzyme protein. Well of porous frit (sintered glass) holds solution in proximity to drop of solution containing protein or enzyme. Vapor from solution in frit controls evaporation of solvent from drop to control precipitation of protein or enzyme. With device, rate of nucleation limited to decrease number and increase size (and perhaps quality) of crystals - large crystals of higher quality needed for x-ray diffraction studies of macromolecules.

[Culture of pancreatic progenitor cells in hanging drop and on floating filter].

PubMed

Ma, Feng-xia; Chen, Fang; Chi, Ying; Yang, Shao-guang; Lu, Shi-hong; Han, Zhong-chao

2013-06-01

To construct a method to culture pancreatic progenitor cells in hanging drop and on floating filter,and to examine if pancreatic progenitor cells can differentiate into mature endocrine cells with this method. Murine embryos at day 12.5 were isolated and digested into single cells,which were then cultured in hanging drop for 24h and formed spheres.Spheres were cultured on the filter for 6 days,which floated in the dish containing medium.During culture,the expressions of pancreas duodenum homeobox-1(PDX-1)and neurogenin3(Ngn3)were determined.The expressions of endocrine and exocrine markers,insulin,glucagon,and carboxypeptidase(CPA)were determined on day 7 by immunohistochemistry.Insulin secretion of spheres stimulated by glucose was detected by ELISA.The changes of pancreatic marker expressions during culture were monitored by real-time polymerase chain reaction(PCR). One day after the culture,there were still a large amount of PDX-1 positive cells in pancreatic spheres,and these cells proliferated.On day 3,high expression of Ngn3 was detected,and the Ngn3-positive cells did not proliferate.On day 7,The expressions of endocrine and exocrine markers in the differentiated pancreatic progenitor cells were detected,which were consistent with that in vivo.Insulin was secreted by spheres upon the stimulation of glucose. In hanging drop and on floating filter,pancreatic progenitor cells can differentiate into mature endocrine cells.

Beyond the First Optical Depth: Fusing Optical Data From Ocean Color Imagery and Gliders

DTIC Science & Technology

2009-01-01

34*/ Office of Counsel,Code 1008.3 U •• "*-<-, ADOR/Director NCST E. R. Franchi , 7000 %. Public Affairs (Unclassified/ Unlimited Only). Code -rn...extreme weather (e.g., hurricanes) becoming a safe and efficient alternative to shipboard surveys3. Despite these benefits , data streams provided by...ECO-triplet poke, WetLabs). Unlike other glider types (e.g., spray, seaglider), the use of Slocums was especially advantageous in the WAP region to

Hanging-wall deformation above a normal fault: sequential limit analyses

NASA Astrophysics Data System (ADS)

Yuan, Xiaoping; Leroy, Yves M.; Maillot, Bertrand

2015-04-01

The deformation in the hanging wall above a segmented normal fault is analysed with the sequential limit analysis (SLA). The method combines some predictions on the dip and position of the active fault and axial surface, with geometrical evolution à la Suppe (Groshong, 1989). Two problems are considered. The first followed the prototype proposed by Patton (2005) with a pre-defined convex, segmented fault. The orientation of the upper segment of the normal fault is an unknown in the second problem. The loading in both problems consists of the retreat of the back wall and the sedimentation. This sedimentation starts from the lowest point of the topography and acts at the rate rs relative to the wall retreat rate. For the first problem, the normal fault either has a zero friction or a friction value set to 25o or 30o to fit the experimental results (Patton, 2005). In the zero friction case, a hanging wall anticline develops much like in the experiments. In the 25o friction case, slip on the upper segment is accompanied by rotation of the axial plane producing a broad shear zone rooted at the fault bend. The same observation is made in the 30o case, but without slip on the upper segment. Experimental outcomes show a behaviour in between these two latter cases. For the second problem, mechanics predicts a concave fault bend with an upper segment dip decreasing during extension. The axial surface rooting at the normal fault bend sees its dips increasing during extension resulting in a curved roll-over. Softening on the normal fault leads to a stepwise rotation responsible for strain partitioning into small blocks in the hanging wall. The rotation is due to the subsidence of the topography above the hanging wall. Sedimentation in the lowest region thus reduces the rotations. Note that these rotations predicted by mechanics are not accounted for in most geometrical approaches (Xiao and Suppe, 1992) and are observed in sand box experiments (Egholm et al., 2007, referring

Generation of Homogenous Three-Dimensional Pancreatic Cancer Cell Spheroids Using an Improved Hanging Drop Technique

PubMed Central

Ware, Matthew J.; Colbert, Kevin; Keshishian, Vazrik; Ho, Jason; Corr, Stuart J.; Curley, Steven A.

2016-01-01

In vitro characterization of tumor cell biology or of potential anticancer drugs is usually performed using tumor cell lines cultured as a monolayer. However, it has been previously shown that three-dimensional (3D) organization of the tumor cells is important to provide insights on tumor biology and transport of therapeutics. Several methods to create 3D tumors in vitro have been proposed, with hanging drop technique being the most simple and, thus, most frequently used. However, in many cell lines this method has failed to form the desired 3D tumor structures. The aim of this study was to design and test an easy-to-use and highly reproducible modification of the hanging drop method for tumor sphere formation by adding methylcellulose polymer. Most pancreatic cancer cells do not form cohesive and manageable spheres when the original hanging drop method is used, thus we investigated these cell lines for our modified hanging drop method. The spheroids produced by this improved technique were analyzed by histology, light microscopy, immunohistochemistry, and scanning electron microscopy. Results show that using the proposed simple method; we were able to produce uniform spheroids for all five of the tested human pancreatic cancer cell lines; Panc-1, BxPC-3, Capan-1, MiaPaCa-2, and AsPC-1. We believe that this method can be used as a reliable and reproducible technique to make 3D cancer spheroids for use in tumor biology research and evaluation of therapeutic responses, and for the development of bio-artificial tissues. PMID:26830354

Generation of Homogenous Three-Dimensional Pancreatic Cancer Cell Spheroids Using an Improved Hanging Drop Technique.

PubMed

Ware, Matthew J; Colbert, Kevin; Keshishian, Vazrik; Ho, Jason; Corr, Stuart J; Curley, Steven A; Godin, Biana

2016-04-01

In vitro characterization of tumor cell biology or of potential anticancer drugs is usually performed using tumor cell lines cultured as a monolayer. However, it has been previously shown that three-dimensional (3D) organization of the tumor cells is important to provide insights on tumor biology and transport of therapeutics. Several methods to create 3D tumors in vitro have been proposed, with hanging drop technique being the most simple and, thus, most frequently used. However, in many cell lines this method has failed to form the desired 3D tumor structures. The aim of this study was to design and test an easy-to-use and highly reproducible modification of the hanging drop method for tumor sphere formation by adding methylcellulose polymer. Most pancreatic cancer cells do not form cohesive and manageable spheres when the original hanging drop method is used, thus we investigated these cell lines for our modified hanging drop method. The spheroids produced by this improved technique were analyzed by histology, light microscopy, immunohistochemistry, and scanning electron microscopy. Results show that using the proposed simple method; we were able to produce uniform spheroids for all five of the tested human pancreatic cancer cell lines; Panc-1, BxPC-3, Capan-1, MiaPaCa-2, and AsPC-1. We believe that this method can be used as a reliable and reproducible technique to make 3D cancer spheroids for use in tumor biology research and evaluation of therapeutic responses, and for the development of bio-artificial tissues.

Evaluation of the Sierra, hanging, quick-don, crew, pressure-breathing, oxygen mask.

DOT National Transportation Integrated Search

1966-06-01

A new design, hanging, quick-don crew pressure breathing oxygen mask applicable to jet-transport operations was tested and evaluated in three phases, consisting of (1) exposing five masked subjects to a stepwise altitude-chamber flight profile with a...

Variable Structure Control of a Hand-Launched Glider

NASA Technical Reports Server (NTRS)

Anderson, Mark R.; Waszak, Martin R.

2005-01-01

Variable structure control system design methods are applied to the problem of aircraft spin recovery. A variable structure control law typically has two phases of operation. The reaching mode phase uses a nonlinear relay control strategy to drive the system trajectory to a pre-defined switching surface within the motion state space. The sliding mode phase involves motion along the surface as the system moves toward an equilibrium or critical point. Analysis results presented in this paper reveal that the conventional method for spin recovery can be interpreted as a variable structure controller with a switching surface defined at zero yaw rate. Application of Lyapunov stability methods show that deflecting the ailerons in the direction of the spin helps to insure that this switching surface is stable. Flight test results, obtained using an instrumented hand-launched glider, are used to verify stability of the reaching mode dynamics.

Estimating cross-slope exchange from drifter tracks and from glider sections

NASA Astrophysics Data System (ADS)

Huthnance, John M.

2017-04-01

In areas of complex topography, it can be difficult to define "along-slope" or "cross-slope" direction, yet transport estimates are sensitive to these definitions, especially as along-slope flow is favoured by geostrophy. However, if drifter positions and hence underlying water depths are recorded regularly, we know where and when depth contours are crossed by the drifters, and hence by the water assuming that the drifters follow the water. An approach is discussed for deriving statistics of contour-crossing speed, via depth changes experienced by the drifters and an effective slope. The transport equation for (e.g.) salinity S can be reduced to an explicit equation for effective diffusivity K if we assume steady along-slope flow with known total transport Q, a salinity maximum at its "core" and effective diffusion to less saline waters to either side. Salinity gradients along the flow and to either side are needed to calculate K. Gliders provide a means of measuring salinity gradients in this context. Measurements at the continental shelf edge south-west of England and west of Scotland illustrate the calculation. Both approaches give overall rather than process-related estimates. There is limited scope for process discrimination according to (i) how often drifter locations are recorded and (ii) the time-intervals into which estimates are "binned". (i) Frequent recording may record more crossings owing to processes of short time scale, albeit these are less significant for slowly-evolving water contents. (ii) Sufficient samples for statistically significant estimates of exchange entail "bins" spanning some weeks or months for typically-limited numbers of drifters or gliders.

White River National Forest Hanging Lake visitor transportation survey : summary of results

DOT National Transportation Integrated Search

2017-01-01

The USDOT Volpe Center conducted a visitor transportation survey at Hanging Lake recreation site in the White River National Forest from July 14 to July 18, 2016. This report outlines the summary of results from that survey effort. Key findings inclu...

[Hangings in the material of Department of Forensic Medicine, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, in the years 2000-2010].

PubMed

Sumińska-Ziemann, Barbara; Bloch-Bogusławska, Elzbieta

2013-01-01

This study presents an analysis of suicides based on autopsy protocols from the years 2000-2010. Out of all autopsies conducted at Department of Forensic Medicine, Nicolaus Copernicus University Collegium Medicum in Bydgoszcz, 477 cases resulted from hanging. During this period the majority of hangings were recorded in 2010 (61 cases). The parameters assessed in this study included age, sex, post-mortem blood ethanol concentration and place of death of the victims. The age range of the deceased was similar in the group of men and women. Males represented the majority of victims of hanging (89%); only 11% of all the victims were females. The authors observed differences in blood alcohol level of the victims in association with their sex. Not present ethanol was noted in approx. 42% of men and 71% of women. More than 65% cases of suicide hangings were encountered in urban areas, in living quarters and outbuildings.

Effect of hang cleans or squats paired with countermovement vertical jumps on vertical displacement.

PubMed

Andrews, Tedi R; Mackey, Theresa; Inkrott, Thomas A; Murray, Steven R; Clark, Ida E; Pettitt, Robert W

2011-09-01

Complex training is characterized by pairing resistance exercise with plyometric exercise to exploit the postactivation potentiation (PAP) phenomenon, thereby promising a better training effect. Studies on PAP as measured by human power performances are equivocal. One issue may be the lack of analyses across multiple sets of paired exercises, a common practice used by athletes. We evaluated countermovement vertical jump (CMJ) performance in 19 women, collegiate athletes in 3 of the following trials: (a) CMJs-only, where 1 set of CMJs served as a conditioning exercise, (b) heavy-load, back squats paired with CMJs, and (c) hang cleans paired with CMJs. The CMJ vertical displacement (3-attempt average), as measured with digital video, served as the dependent variable of CMJ performance. Across 3 sets of paired-exercise regimens, CMJ-only depreciated 1.6 cm and CMJ paired with back squats depreciated 2.0 cm (main effect, p < 0.05). Conversely, CMJ paired with hang cleans depreciated 0.30 cm (interaction, p < 0.05). Thus, the best complex training scheme was achieved by pairing CMJs with hang cleans in comparison to back squats or CMJs in and of themselves. Future research on exercise modes of complex training that best help athletes preserve and train with the highest power possible, in a given training session, is warranted.

Micro-Ring Structures Stabilize Microdroplets to Enable Long Term Spheroid Culture in 384 Hanging Drop Array Plates

PubMed Central

Hsiao, Amy Y.; Tung, Yi-Chung; Kuo, Chuan-Hsien; Mosadegh, Bobak; Bedenis, Rachel; Pienta, Kenneth J.; Takayama, Shuichi

2012-01-01

Using stereolithography, 20 different structural variations comprised of millimeter diameter holes surrounded by trenches, plateaus, or micro-ring structures were prepared and tested for their ability to stably hold arrays of microliter sized droplets within the structures over an extended period of time. The micro-ring structures were the most effective in stabilizing droplets against mechanical and chemical perturbations. After confirming the importance of micro-ring structures using rapid prototyping, we developed an injection molding tool for mass production of polystyrene 3D cell culture plates with an array of 384 such micro-ring surrounded through-hole structures. These newly designed and injection molded polystyrene 384 hanging drop array plates with micro-rings were stable and robust against mechanical perturbations as well as surface fouling-facilitated droplet spreading making them capable of long term cell spheroid culture of up to 22 days within the droplet array. This is a significant improvement over previously reported 384 hanging drop array plates which are susceptible to small mechanical shocks and could not reliably maintain hanging drops for longer than a few days. With enhanced droplet stability, the hanging drop array plates with micro-ring structures provide better platforms and open up new opportunities for high-throughput preparation of microscale 3D cell constructs for drug screening and cell analysis. PMID:22057945

Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates.

PubMed

Hsiao, Amy Y; Tung, Yi-Chung; Kuo, Chuan-Hsien; Mosadegh, Bobak; Bedenis, Rachel; Pienta, Kenneth J; Takayama, Shuichi

2012-04-01

Using stereolithography, 20 different structural variations comprised of millimeter diameter holes surrounded by trenches, plateaus, or micro-ring structures were prepared and tested for their ability to stably hold arrays of microliter sized droplets within the structures over an extended period of time. The micro-ring structures were the most effective in stabilizing droplets against mechanical and chemical perturbations. After confirming the importance of micro-ring structures using rapid prototyping, we developed an injection molding tool for mass production of polystyrene 3D cell culture plates with an array of 384 such micro-ring surrounded through-hole structures. These newly designed and injection molded polystyrene 384 hanging drop array plates with micro-rings were stable and robust against mechanical perturbations as well as surface fouling-facilitated droplet spreading making them capable of long term cell spheroid culture of up to 22 days within the droplet array. This is a significant improvement over previously reported 384 hanging drop array plates which are susceptible to small mechanical shocks and could not reliably maintain hanging drops for longer than a few days. With enhanced droplet stability, the hanging drop array plates with micro-ring structures provide better platforms and open up new opportunities for high-throughput preparation of microscale 3D cell constructs for drug screening and cell analysis.

THE SUGAR GLIDER (PETAURUS BREVICEPS): A LABORATORY HOST FOR THE NEMATODE PARASTRONGYLOIDES TRICHOSURI

PubMed Central

Nolan, Thomas J.; Zhu, Xiaodong; Ketschek, Andrea; Cole, Joan; Grant, Warwick; Lok, James B.; Schad, Gerhard A.

2011-01-01

Parastrongyloides trichosuri is a nematode parasite of the Australian brush-tailed possums that can be propagated through many generations in vitro. This makes P. trichosuri uniquely suited for genetic investigations, including those involving transgenesis. However, an obstacle to its use as an experimental model has been the fact that its host is limited to Australia and New Zealand and that it cannot be exported because of its status as a protected species or agricultural pest, respectively. In previous studies, conventional laboratory animals such as rats, mice, rabbits, ferrets, and chickens have failed to support infections. In the present study, gerbils and short-tailed opossums proved similarly refractory to infection. In contrast, the sugar glider (Petaurus breviceps, family Petauridae) proved to be a good host for P. trichosuri. Patent infections resulted using as few as 6 infective larvae (L3i) and as many as 2,000 L3i. Large numbers of L3i (1,000–2,000) produced patent infections of much shorter duration than those seen when 100 L3i were initially given to the sugar glider. In one case, an infection initiated with 100 L3i was patent for over 1 yr. Parastrongyloides trichosuri is easily cryopreserved using a method developed for Strongyloides stercoralis. Thus, we have identified an experimental host for P. trichosuri that will make it possible to conduct research on this parasite in laboratories outside the endemic sites. PMID:18163342

The sugar glider (Petaurus breviceps): a laboratory host for the nematode Parastrongyloides trichosuri.

PubMed

Nolan, Thomas J; Zhu, Xiaodong; Ketschek, Andrea; Cole, Joan; Grant, Warwick; Lok, James B; Schad, Gerhard A

2007-10-01

Parastrongyloides trichosuri is a nematode parasite of the Australian brush-tailed possums that can be propagated through many generations in vitro. This makes P. trichosuri uniquely suited for genetic investigations, including those involving transgenesis. However, an obstacle to its use as an experimental model has been the fact that its host is limited to Australia and New Zealand and that it cannot be exported because of its status as a protected species or agricultural pest, respectively. In previous studies, conventional laboratory animals such as rats, mice, rabbits, ferrets, and chickens have failed to support infections. In the present study, gerbils and short-tailed opossums proved similarly refractory to infection. In contrast, the sugar glider (Petaurus breviceps, family Petauridae) proved to be a good host for P. trichosuri. Patent infections resulted using as few as 6 infective larvae (L3i) and as many as 2,000 L3i. Large numbers of L3i (1,000-2,000) produced patent infections of much shorter duration than those seen when 100 L3i were initially given to the sugar glider. In one case, an infection initiated with 100 L3i was patent for over 1 yr. Parastrongyloides trichosuri is easily cryopreserved using a method developed for Strongyloides stercoralis. Thus, we have identified an experimental host for P. trichosuri that will make it possible to conduct research on this parasite in laboratories outside the endemic sites.

Measuring pH variability using an experimental sensor on an underwater glider

NASA Astrophysics Data System (ADS)

Hemming, Michael P.; Kaiser, Jan; Heywood, Karen J.; Bakker, Dorothee C. E.; Boutin, Jacqueline; Shitashima, Kiminori; Lee, Gareth; Legge, Oliver; Onken, Reiner

2017-05-01

Autonomous underwater gliders offer the capability of measuring oceanic parameters continuously at high resolution in both vertical and horizontal planes, with timescales that can extend to many months. An experimental ion-sensitive field-effect transistor (ISFET) sensor measuring pH on the total scale was attached to a glider during the REP14-MED experiment in June 2014 in the Sardinian Sea in the northwestern Mediterranean. During the deployment, pH was sampled at depths of up to 1000 m along an 80 km transect over a period of 12 days. Water samples were collected from a nearby ship and analysed for dissolved inorganic carbon concentration and total alkalinity to derive the pH for validating the ISFET sensor measurements. The vertical resolution of the pH sensor was good (1 to 2 m), but stability was poor and the sensor drifted in a non-monotonous fashion. In order to remove the sensor drift, a depth-constant time-varying offset was applied throughout the water column for each dive, reducing the spread of the data by approximately two-thirds. Furthermore, the ISFET sensor required temperature- and pressure-based corrections, which were achieved using linear regression. Correcting for this decreased the apparent sensor pH variability by a further 13 to 31 %. Sunlight caused an apparent sensor pH decrease of up to 0.1 in surface waters around local noon, highlighting the importance of shielding the sensor from light in future deployments. The corrected pH from the ISFET sensor is presented along with potential temperature, salinity, potential density anomalies (σθ), and dissolved oxygen concentrations (c(O2)) measured by the glider, providing insights into the physical and biogeochemical variability in the Sardinian Sea. The pH maxima were identified close to the depth of the summer chlorophyll maximum, where high c(O2) values were also found. Longitudinal pH variations at depth (σθ > 28. 8 kg m-3) highlighted the variability of water masses in the Sardinian

Flight of Sharovipteryx mirabilis: the world's first delta-winged glider.

PubMed

Dyke, G J; Nudds, R L; Rayner, J M V

2006-07-01

The 225 million-year-old reptile Sharovipteryx mirabilis was the world's first delta-winged glider; this remarkable animal had a flight surface composed entirely of a hind-limb membrane. We use standard delta-wing aerodynamics to reconstruct the flight of S. mirabilis demonstrating that wing shape could have been controlled simply by protraction of the femora at the knees, and by variation in incidence of a small forelimb canard. Our method has allowed us to address the question of how identifying realistic glide performance can be used to set limits on aerodynamic design in this small animal. Our novel interpretation of the bizarre flight mode of S. mirabilis is the first based directly on interpretation of the fossil itself and the first grounded in aerodynamics.

Increase in Suicide Rates by Hanging in the Population of Tabasco, Mexico between 2003 and 2012.

PubMed

Hernández-Alvarado, Mervyn Manuel; González-Castro, Thelma Beatriz; Tovilla-Zárate, Carlos Alfonso; Fresán, Ana; Juárez-Rojop, Isela E; López-Narváez, María Lilia; Villar-Soto, Mario; Genis-Mendoza, Alma

2016-06-01

Worldwide, the suicide rate is decreasing. To examine changes in the rates of completed suicide in the Mexican population from 2003 to 2012, we analyzed these changes according to: (i) the method of suicide; (ii) age group and (iii) gender. The data analyzed were obtained from governmental organizations from the State of Tabasco, Mexico. The data provided 1836 cases of subjects born and residing in Tabasco, who completed suicide in this state. Suicide by hanging was a common choice of suicide method for Mexicans. The rate of suicide by hanging increased from 5.80 to 6.49 per 100,000 persons between 2003 and 2012, a rate percentage increase of 11.89%. Hanging was found to be the most common choice of suicide in the Mexican population, probably because the materials required are easily available and the method does not require complicated techniques, especially in the 55-64 age group. Strategies for prevention and intervention should be developed for the Mexican population considering suicide rates by age group and gender.

Increase in Suicide Rates by Hanging in the Population of Tabasco, Mexico between 2003 and 2012

PubMed Central

Hernández-Alvarado, Mervyn Manuel; González-Castro, Thelma Beatriz; Tovilla-Zárate, Carlos Alfonso; Fresán, Ana; Juárez-Rojop, Isela E.; López-Narváez, María Lilia; Villar-Soto, Mario; Genis-Mendoza, Alma

2016-01-01

Background: Worldwide, the suicide rate is decreasing. To examine changes in the rates of completed suicide in the Mexican population from 2003 to 2012, we analyzed these changes according to: (i) the method of suicide; (ii) age group and (iii) gender. Methods: The data analyzed were obtained from governmental organizations from the State of Tabasco, Mexico. The data provided 1836 cases of subjects born and residing in Tabasco, who completed suicide in this state. Results: Suicide by hanging was a common choice of suicide method for Mexicans. The rate of suicide by hanging increased from 5.80 to 6.49 per 100,000 persons between 2003 and 2012, a rate percentage increase of 11.89%. Conclusions: Hanging was found to be the most common choice of suicide in the Mexican population, probably because the materials required are easily available and the method does not require complicated techniques, especially in the 55–64 age group. Strategies for prevention and intervention should be developed for the Mexican population considering suicide rates by age group and gender. PMID:27258292

International Technology Transfer the Rope to Hang the West

DTIC Science & Technology

1989-03-28

order to provide awareness and appreciation of its importance to the security of the United States. DO R 1473 EOfTION OF V NOV 65 I.; OBSOLETE E - 7I... e Data Eme’e, USAWC MILITARY STUDIES PROGRAM PAPER INTERNATIONAL TECHNOLOGY TRANSFER The Rope To Hang The West AN INDIVIDUAL STUDY PROJECT Intended...notably the Departments of State, Commerce and Defense), and other friendly nations at odds with each other over competing demands and parochial interests

Modeling and Simulation of Control Actuation System with Fuzzy-PID Logic Controlled Brushless Motor Drives for Missiles Glider Applications

PubMed Central

Muniraj, Murali; Arulmozhiyal, Ramaswamy

2015-01-01

A control actuation system has been used extensively in automotive, aerospace, and defense applications. The major challenges in modeling control actuation system are rise time, maximum peak to peak overshoot, and response to nonlinear system with percentage error. This paper addresses the challenges in modeling and real time implementation of control actuation system for missiles glider applications. As an alternative fuzzy-PID controller is proposed in BLDC motor drive followed by linkage mechanism to actuate fins in missiles and gliders. The proposed system will realize better rise time and less overshoot while operating in extreme nonlinear dynamic system conditions. A mathematical model of BLDC motor is derived in state space form. The complete control actuation system is modeled in MATLAB/Simulink environment and verified by performing simulation studies. A real time prototype of the control actuation is developed with dSPACE-1104 hardware controller and a detailed analysis is carried out to confirm the viability of the proposed system. PMID:26613102

Modeling and Simulation of Control Actuation System with Fuzzy-PID Logic Controlled Brushless Motor Drives for Missiles Glider Applications.

PubMed

Muniraj, Murali; Arulmozhiyal, Ramaswamy

2015-01-01

A control actuation system has been used extensively in automotive, aerospace, and defense applications. The major challenges in modeling control actuation system are rise time, maximum peak to peak overshoot, and response to nonlinear system with percentage error. This paper addresses the challenges in modeling and real time implementation of control actuation system for missiles glider applications. As an alternative fuzzy-PID controller is proposed in BLDC motor drive followed by linkage mechanism to actuate fins in missiles and gliders. The proposed system will realize better rise time and less overshoot while operating in extreme nonlinear dynamic system conditions. A mathematical model of BLDC motor is derived in state space form. The complete control actuation system is modeled in MATLAB/Simulink environment and verified by performing simulation studies. A real time prototype of the control actuation is developed with dSPACE-1104 hardware controller and a detailed analysis is carried out to confirm the viability of the proposed system.

[A homicidal strangulation by ligature, disguised as a suicidal hanging].

PubMed

Ueno, Y; Fukunaga, T; Nakagawa, K; Imabayashi, T; Fujiwara, S; Adachi, J; Mizoi, Y

1989-02-01

A naked 38-year-old woman was found dead, lying beside a bed in a guest room of a hotel. A cotton cord was looped around her neck, with one end tied to a bedpost. Thus it seemed that she had hanged herself in a lying position. Yet, certain suspicious factors gave rise to doubts. The hair and a necklace were intertwined with the cord and a man that had been staying in the same room had fled. On the postmortem examination, marked congestion and many petechial haemorrhages were seen in the face and the upper area of the neck, also in conjunctiva. A light-brown and highly dry ligature mark, about one centimeter in width, was found on the neck. The mark, crossing the front of the neck, was clearly evident from the back to the right side of the neck. The lower borders of the congestion and petechial haemorrhages in the face and neck were located 1 or 2 centimeters above the ligature mark. On the left side of the neck, another pale ligature mark with slight subcutaneous haemorrhages was seen between the dry mark and the lower border of the congested part of the neck. A similar ligature mark also was found on the back of the neck. On internal examination, congestion of the cervical lymph nodes and haemorrhages in the cervical muscles were recognized in the areas of the cutaneous congestion. These findings supported suspicions that the dry ligature mark had been caused by hanging after her death, and that the light ligature mark with slight subcutaneous haemorrhages on the left side and back of the neck had been caused by strangulation, which had brought on the victim's death. Therefore, it was decided that her death had been disguised as a suicidal hanging, after she had been strangled by ligature. A week later, her former husband was arrested and confessed his crime.

Liver hanging maneuver for right hemiliver in situ donation--anatomical considerations.

PubMed

Trotovsek, B; Gadzijev, E M; Ravnik, D; Hribernik, M

2006-01-01

An anatomical study was carried out to evaluate the safety of the liver hanging maneuver for the right hemiliver in living donor and in situ splitting transplantation. During this procedure a 4-6 cm blind dissection is performed between the inferior vena cava and the liver. Short subhepatic veins entering the inferior vena cava from segments 1 and 9 could be torn with consequent hemorrhage. One hundred corrosive casts of livers were evaluated to establish the position and diameter of short subhepatic veins and the inferior right hepatic vein. The average distance from the right border of the inferior vena cava to the opening of segment 1 veins was 16.7+/-3.4 mm and to the entrance of segment 9 veins was 5.0+/-0.5 mm. The width of the narrowest point on the route of blind dissection was determined, with the average value being 8.7+/-2.3 mm (range 2-15 mm). The results show that the liver hanging maneuver is a safe procedure. A proposed route of dissection minimizes the risk of disrupting short subhepatic veins (7%).

The preparation of Drosophila embryos for live-imaging using the hanging drop protocol.

PubMed

Reed, Bruce H; McMillan, Stephanie C; Chaudhary, Roopali

2009-03-13

Green fluorescent protein (GFP)-based timelapse live-imaging is a powerful technique for studying the genetic regulation of dynamic processes such as tissue morphogenesis, cell-cell adhesion, or cell death. Drosophila embryos expressing GFP are readily imaged using either stereoscopic or confocal microscopy. A goal of any live-imaging protocol is to minimize detrimental effects such as dehydration and hypoxia. Previous protocols for preparing Drosophila embryos for live-imaging analysis have involved placing dechorionated embryos in halocarbon oil and sandwiching them between a halocarbon gas-permeable membrane and a coverslip. The introduction of compression through mounting embryos in this manner represents an undesirable complication for any biomechanical-based analysis of morphogenesis. Our method, which we call the hanging drop protocol, results in excellent viability of embryos during live imaging and does not require that embryos be compressed. Briefly, the hanging drop protocol involves the placement of embryos in a drop of halocarbon oil that is suspended from a coverslip, which is, in turn, fixed in position over a humid chamber. In addition to providing gas exchange and preventing dehydration, this arrangement takes advantage of the buoyancy of embryos in halocarbon oil to prevent them from drifting out of position during timelapse acquisition. This video describes in detail how to collect and prepare Drosophila embryos for live imaging using the hanging drop protocol. This protocol is suitable for imaging dechorionated embryos using stereomicroscopy or any upright compound fluorescence microscope.

Application of Hanging Drop Technique for Kidney Tissue Culture.

PubMed

Wang, Shaohui; Wang, Ximing; Boone, Jasmine; Wie, Jin; Yip, Kay-Pong; Zhang, Jie; Wang, Lei; Liu, Ruisheng

2017-01-01

The hanging drop technique is a well-established method used in culture of animal tissues. However, this method has not been used in adult kidney tissue culture yet. This study was to explore the feasibility of using this technique for culturing adult kidney cortex to study the time course of RNA viability in the tubules and vasculature, as well as the tissue structural integrity. In each Petri dish with the plate covered with sterile buffer, a section of mouse renal cortex was cultured within a drop of DMEM culture medium on the inner surface of the lip facing downward. The tissue were then harvested at each specific time points for Real-time PCR analysis and histological studies. The results showed that the mRNA level of most Na+ related transporters and cotransporters were stably maintained within 6 hours in culture, and that the mRNA level of most receptors found in the vasculature and glomeruli were stably maintained for up to 9 days in culture. Paraffin sections of the cultured renal cortex indicated that the tubules began to lose tubular integrity after 6 hours, but the glomeruli and vasculatures were still recognizable up to 9 days in culture. We concluded that adult kidney tissue culture by hanging drop method can be used to study gene expressions in vasculature and glomeruli. © 2017 The Author(s). Published by S. Karger AG, Basel.

Digital microfluidics for automated hanging drop cell spheroid culture.

PubMed

Aijian, Andrew P; Garrell, Robin L

2015-06-01

Cell spheroids are multicellular aggregates, grown in vitro, that mimic the three-dimensional morphology of physiological tissues. Although there are numerous benefits to using spheroids in cell-based assays, the adoption of spheroids in routine biomedical research has been limited, in part, by the tedious workflow associated with spheroid formation and analysis. Here we describe a digital microfluidic platform that has been developed to automate liquid-handling protocols for the formation, maintenance, and analysis of multicellular spheroids in hanging drop culture. We show that droplets of liquid can be added to and extracted from through-holes, or "wells," and fabricated in the bottom plate of a digital microfluidic device, enabling the formation and assaying of hanging drops. Using this digital microfluidic platform, spheroids of mouse mesenchymal stem cells were formed and maintained in situ for 72 h, exhibiting good viability (>90%) and size uniformity (% coefficient of variation <10% intraexperiment, <20% interexperiment). A proof-of-principle drug screen was performed on human colorectal adenocarcinoma spheroids to demonstrate the ability to recapitulate physiologically relevant phenomena such as insulin-induced drug resistance. With automatable and flexible liquid handling, and a wide range of in situ sample preparation and analysis capabilities, the digital microfluidic platform provides a viable tool for automating cell spheroid culture and analysis. © 2014 Society for Laboratory Automation and Screening.

Using in-situ Glider Data to Improve the Interpretation of Time-Series Data in the San Pedro Channel

NASA Astrophysics Data System (ADS)

Teel, E.; Liu, X.; Seegers, B. N.; Ragan, M. A.; Jones, B. H.; Levine, N. M.

2016-02-01

Oceanic time-series have provided insight into biological, physical, and chemical processes and how these processes change over time. However, time-series data collected near coastal zones have not been used as broadly because of regional features that may prevent extrapolation of local results. Though these sites are inherently more affected by local processes, broadening the application of coastal data is crucial for improved modeling of processes such as total carbon drawdown and the development of oxygen minimum zones. Slocum gliders were deployed off the coast of Los Angeles from February to July of 2013 and 2014 providing high temporal and spatial resolution data of the San Pedro Channel (SPC), which includes the San Pedro Ocean Time Series (SPOT). The data were collapsed onto a standardized grid and primary and secondary characteristics of glider profiles were analyzed by principal component analysis to determine the processes impacting SPC and SPOT. The data fell into four categories: active upwelling, offshore intrusion, subsurface bloom, and surface bloom. Waters across the SPC were most similar to offshore water masses, even during the upwelling season when near-shore blooms are commonly observed. The SPOT site was found to be representative of the SPC 86% of the time, suggesting that the findings from SPOT are applicable for the entire SPC. Subsurface blooms were common in both years with co-located chlorophyll and particle maxima, and results suggested that these subsurface blooms contribute significantly to the local primary production. Satellite estimation of integrated chlorophyll was poor, possibly due to the prevalence of subsurface blooms and shallow optical depths during surface blooms. These results indicate that high resolution in-situ glider deployments can be used to determine the spatial domain of coastal time-series data, allowing for broader application of these datasets and greater integration into modeling efforts.

Thermostatic tissue platform for intravital microscopy: 'the hanging drop' model.

PubMed

Pavlovic, Dragan; Frieling, Helge; Lauer, Kai-Stephan; Bac, Vo Hoai; Richter, Joern; Wendt, Michael; Lehmann, Christian; Usichenko, Taras; Meissner, Konrad; Gruendling, Matthias

2006-11-01

Intravital microscopy imposes the particular problem of the combined control of the body temperature of the animal and the local temperature of the observed organ or tissues. We constructed and tested, in the rat ileum microcirculation preparation, a new organ-support platform. The platform consisted of an organ bath filled with physiological solution, and contained a suction tube, a superfusion tube, an intestine-support hand that was attached to a micromanipulator and a thermometer probe. To cover the intestine we used a cover glass plate with a plastic ring glued on its upper surface. After a routine procedure (anaesthesia, monitoring and surgery), the intestine segment (2-3 cm long) was gently exteriorized and placed on the 'hand' of the organ support. A small part of the intestine formed a small 'island' in the bath that was filled with physiological salt solution. The cover glass was secured in place. The physiological salt solution from the superfusion tube, which was pointed to the lower surface of the cover glass, formed a 'hanging drop'. The objective of the microscope was then immersed into distilled water that was formed by the cover glass plastic ring. The 'hanging drop' technique prevented any tissue quenching, ensured undisturbed microcirculation, provided for stable temperature and humidity, and permitted a clear visual field.

Gliders Measure Western Boundary Current Transport from the South Pacific to the Equator

NASA Astrophysics Data System (ADS)

Davis, R. E.; Kessler, W. S.; Sherman, J. T.

2011-12-01

Since 2007, the Consortium on the Ocean's Role in Climate (CORC) has used repeated glider transects across the southern Solomon Sea to measure the previously nearly unsampled mass and heat transport from the South Pacific to the equatorial zone. Mean transport is dominated by the New Guinea Coastal Undercurrent (NGCUC). This low-latitude western boundary current is a major element of the shallow meridional overturning circulation, returning water from the subtropical South Pacific to the Equatorial Undercurrent (EUC) where it upwells. We find the mean NGCUC to be a jet less than 100 km wide, centered near 300 m depth, with equatorward velocities reaching 35 cm/s and salinity anomalies on isopycnals up to 0.05. Weaker poleward flow is found near the surface in the eastern basin. Equatorward transport above 700 m is typically 20 Sv, but nearly vanished during two La Niñas and reached 25 Sv during an El Niño. Within these events the seasonal cycle cannot yet be defined. Transport variability is strongest outside the boundary current and appears to consist of two independently moving layers with a boundary near 250 m. ENSO variability is predominantly in the upper layer. The relation of Solomon Sea mass and heat transport with ENSO indicators will be discussed The ability to initiate and maintain measurements that support such quantitative analyses with a small effort in a remote site far from research institutions demonstrates that gliders can be a productive part of the global ocean observing system.

Suicidal hanging donors for lung transplantation: Is this chapter still closed? Midterm experience from a single center in United Kingdom.

PubMed

Ananiadou, Olga; Schmack, Bastian; Zych, Bartlomiej; Sabashnikov, Anton; Garcia-Saez, Diana; Mohite, Prashant; Weymann, Alexander; Mansur, Ashham; Zeriouh, Mohamed; Marczin, Nandor; De Robertis, Fabio; Simon, Andre Rüdiger; Popov, Aron-Frederik

2018-04-01

In the context of limited donor pool in cardiothoracic transplantation, utilization of organs from high risk donors, such as suicidal hanging donors, while ensuring safety, is under consideration. We sought to evaluate the outcomes of lung transplantations (LTx) that use organs from this group.Between January 2011 and December 2015, 265 LTx were performed at our center. Twenty-two recipients received lungs from donors after suicidal hanging (group 1). The remaining 243 transplantations were used as a control (group 2). Analysis of recipient and donor characteristics as well as outcomes was performed.No statistically significant difference was found in the donor characteristics between analyzed groups, except for higher incidence of cardiac arrest, younger age and smoking history of hanging donors (P < .001, P = .022 and P = .0042, respectively). Recipient preoperative and perioperative characteristics were comparable. Postoperatively in group 1 there was a higher incidence of extracorporeal life support (27.3 vs 9.1%, P = .019). There were no significant differences in chronic lung allograft dysfunction-free survival between group 1 and 2: 92.3 vs 94% at 1 year and 65.9 vs 75.5% at 3 years (P = .99). The estimated cumulative survival rate was also similar between groups: 68.2 vs 83.2% at 1 year and 68.2% versus 72% at 3 years (P = .3758).Hanging as a donor cause of death is not associated with poor mid-term survival or chronic lung allograft dysfunction following transplantation. These results encourage assessment of lungs from hanging donors, and their consideration for transplantation.

Transfer, Imaging, and Analysis Plate for Facile Handling of 384 Hanging Drop 3D Tissue Spheroids

PubMed Central

Cavnar, Stephen P.; Salomonsson, Emma; Luker, Kathryn E.; Luker, Gary D.; Takayama, Shuichi

2014-01-01

Three-dimensional culture systems bridge the experimental gap between in vivo and in vitro physiology. However, nonstandardized formation and limited downstream adaptability of 3D cultures have hindered mainstream adoption of these systems for biological applications, especially for low- and moderate-throughput assays commonly used in biomedical research. Here we build on our recent development of a 384-well hanging drop plate for spheroid culture to design a complementary spheroid transfer and imaging (TRIM) plate. The low-aspect ratio wells of the TRIM plate facilitated highfidelity, user-independent, contact-based collection of hanging drop spheroids. Using the TRIM plate, we demonstrated several downstream analyses, including bulk tissue collection for flow cytometry, high-resolution low working-distance immersion imaging, and timely reagent delivery for enzymatic studies. Low working-distance multiphoton imaging revealed a cell type–dependent, macroscopic spheroid structure. Unlike ovarian cancer spheroids, which formed loose, disk-shaped spheroids, human mammary fibroblasts formed tight, spherical, and nutrient-limited spheroids. Beyond the applications we describe here, we expect the hanging drop spheroid plate and complementary TRIM plate to facilitate analyses of spheroids across the spectrum of throughput, particularly for bulk collection of spheroids and high-content imaging. PMID:24051516

Transfer, imaging, and analysis plate for facile handling of 384 hanging drop 3D tissue spheroids.

PubMed

Cavnar, Stephen P; Salomonsson, Emma; Luker, Kathryn E; Luker, Gary D; Takayama, Shuichi

2014-04-01

Three-dimensional culture systems bridge the experimental gap between in vivo and in vitro physiology. However, nonstandardized formation and limited downstream adaptability of 3D cultures have hindered mainstream adoption of these systems for biological applications, especially for low- and moderate-throughput assays commonly used in biomedical research. Here we build on our recent development of a 384-well hanging drop plate for spheroid culture to design a complementary spheroid transfer and imaging (TRIM) plate. The low-aspect ratio wells of the TRIM plate facilitated high-fidelity, user-independent, contact-based collection of hanging drop spheroids. Using the TRIM plate, we demonstrated several downstream analyses, including bulk tissue collection for flow cytometry, high-resolution low working-distance immersion imaging, and timely reagent delivery for enzymatic studies. Low working-distance multiphoton imaging revealed a cell type-dependent, macroscopic spheroid structure. Unlike ovarian cancer spheroids, which formed loose, disk-shaped spheroids, human mammary fibroblasts formed tight, spherical, and nutrient-limited spheroids. Beyond the applications we describe here, we expect the hanging drop spheroid plate and complementary TRIM plate to facilitate analyses of spheroids across the spectrum of throughput, particularly for bulk collection of spheroids and high-content imaging.

An analysis of the predictors of mortality and morbidity in patients admitted after suicidal hanging to an Indian multidisciplinary Intensive Care Unit

PubMed Central

Renuka, MK; Kalaiselvan, MS; Arunkumar, AS

2017-01-01

Background and Aims: Hanging is a frequently used method to attempt suicide in India. There is a lack of data in the Indian population regarding clinical features and outcomes of suicidal hanging. The purpose of this study was to evaluate the factors affecting mortality and morbidity in patients admitted with suicidal hanging to the Intensive Care Unit (ICU). Methods: A 6-year retrospective study of adult patients admitted to the ICU with suicidal hanging was analysed for demographics, mode of hanging, lead time to emergency room (ER) admission, clinical presentation, Acute Physiology and Chronic Health Evaluation II (APACHE II) and Sequential Organ Failure Assessment (SOFA) scores, admission Glasgow coma scale (GCS) and neurological outcomes. The primary outcome was in-hospital mortality rate. Secondary outcomes were hospital length of stay (LOS), ICU-LOS, time for neurological recovery, organ support and duration of mechanical ventilation. Statistical analysis was performed using the Student's t-test for continuous variables and Chi-square test for categorical variables. Results: We analysed data of 106 patients. The median age was 27 years [Interquartile Range (IQR) (22–34)]. The median lead time to ER admission was 1 h [IQR (0.5–1.4)] with median ICU stay of 3 days [IQR (2–4)]. Vasopressors were administered to 27.4% patients. GCS was ≤7 in 65% patients, and 84.9% patients received mechanical ventilation. Mortality rate was 10.3%. Survivors recovered with normal organ function. Conclusion: Suicidal hanging is associated with significant mortality. Admission GCS, APACHE II and 48 h SOFA score were predictors of poor outcome. PMID:28794524

Investigating the spring bloom initiation and net community production in the Subantarctic Southern Ocean using high-resolution in situ glider data

NASA Astrophysics Data System (ADS)

thomalla, sandy; Racault, Marie-Fanny; Swart, Sebastiaan; Monteiro, Pedro

2014-05-01

Phytoplankton bloom phenology has important consequences for marine ecosystems, fisheries and carbon export to the ocean interior. As such, it is important to examine the drivers of phytoplankton bloom initiation and their sensitivity to inter-annual climate variability and change. In this study we use ~6 months of in-situ high-resolution glider data to investigate the spring bloom initiation in the subantarctic zone (SAZ) of the Southern Ocean by implementing three different methods; a rate of change method, a threshold method and a cumulative sum method. The bloom initiation dates are critically compared to one another and the drivers of discrepancies assessed to inform on the sensitivities of different methods to processes driving the seasonal evolution of phytoplankton biomass in the subantarctic. The bloom initiation dates combined with in situ glider data of chlorophyll, light, and mixed layer depth allow us to resolve both Sverdrup's Critical Depth and Behrenfeld's Disturbance Recovery models through the water column and thus determine the seasonal evolution of net community production and respiration rates and the potential for carbon export. The outputs of the two different models are compared to one another in the context of their sensitivities to water column processes thereby refining their ability to address specific system scale questions. The novelty of this study is that gliders provide an unprecedented dataset to assess the seasonal cycle of phytoplankton biomass throughout the water column at high resolution, thus enhancing our understanding of net community production and export processes at submeso-space and sub-seasonal time scales.

Toxicology findings in cases of hanging in the City and County of San Francisco over the 3-year period from 2011 to 2013.

PubMed

San Nicolas, A C; Lemos, N P

2015-10-01

In postmortem cases where the cause of death is hanging, toxicological analyses may be considered unnecessary by some medical examiners, toxicologists, and other persons involved in medico-legal investigations because the cause of death seems "obvious." To ascertain if toxicological analyses are necessary when the cause of death is hanging, all 102 hanging cases (25 females; 77 males) from 2011 to 2013 that came under the jurisdiction of the San Francisco Office of the Chief Medical Examiner were examined from a total of 3912 sudden, unexpected, or violent death cases in the same period. Suicide was the manner of death in 99 of these cases, with two accidental and one undetermined death. The average age of decedents was 43.9 years (median 41), the youngest was an 11-year old male and the oldest was an 86-year old female. Of the 102 cases, 33 had negative toxicology while 69 cases had at least one positive toxicology result. Females were equally likely to have negative or positive results (12 and 13 cases respectively), but males were 37.5% more likely to have positive toxicology (n=56) rather than negative toxicology (n=21). For females, alcohol, mirtazapine, venlafaxine, and trazodone were the top psychoactive substances in peripheral blood while THC, cocaine, hydrocodone, bupropion, olanzapine, doxylamine, quetiapine and dextromethorphan were also reported. For males, alcohol, THC, cocaine, amphetamine, methamphetamine, bupropion, and diphenhydramine were the top psychoactive substances in blood, but several other drugs were also found in individual cases. Our study of hanging cases over a 3-year period support the idea that complete postmortem toxicology investigation of hangings should be performed, even when the "obvious" cause of death is asphyxia due to hanging. Many of these cases involved psychoactive substances (most often alcohol and cannabis), and having such knowledge provides a better understanding of the circumstances surrounding the decedent's death

SEADYN Analysis of a Tow Line for a High Altitude Towed Glider

NASA Technical Reports Server (NTRS)

Colozza, Anthony J.

1996-01-01

The concept of using a system, consisting of a tow aircraft, glider and tow line, which would enable subsonic flight at altitudes above 24 km (78 kft) has previously been investigated. The preliminary results from these studies seem encouraging. Under certain conditions these studies indicate the concept is feasible. However, the previous studies did not accurately take into account the forces acting on the tow line. Therefore in order to investigate the concept further a more detailed analysis was needed. The code that was selected was the SEADYN cable dynamics computer program which was developed at the Naval Facilities Engineering Service Center. The program is a finite element based structural analysis code that was developed over a period of 10 years. The results have been validated by the Navy in both laboratory and at actual sea conditions. This code was used to simulate arbitrarily-configured cable structures subjected to excitations encountered in real-world operations. The Navy's interest was mainly for modeling underwater tow lines, however the code is also usable for tow lines in air when the change in fluid properties is taken into account. For underwater applications the fluid properties are basically constant over the length of the tow line. For the tow aircraft/glider application the change in fluid properties is considerable along the length of the tow line. Therefore the code had to be modified in order to take into account the variation in atmospheric properties that would be encountered in this application. This modification consisted of adding a variable density to the fluid based on the altitude of the node being calculated. This change in the way the code handled the fluid density had no effect on the method of calculation or any other factor related to the codes validation.

High accuracy navigation information estimation for inertial system using the multi-model EKF fusing adams explicit formula applied to underwater gliders.

PubMed

Huang, Haoqian; Chen, Xiyuan; Zhang, Bo; Wang, Jian

2017-01-01

The underwater navigation system, mainly consisting of MEMS inertial sensors, is a key technology for the wide application of underwater gliders and plays an important role in achieving high accuracy navigation and positioning for a long time of period. However, the navigation errors will accumulate over time because of the inherent errors of inertial sensors, especially for MEMS grade IMU (Inertial Measurement Unit) generally used in gliders. The dead reckoning module is added to compensate the errors. In the complicated underwater environment, the performance of MEMS sensors is degraded sharply and the errors will become much larger. It is difficult to establish the accurate and fixed error model for the inertial sensor. Therefore, it is very hard to improve the accuracy of navigation information calculated by sensors. In order to solve the problem mentioned, the more suitable filter which integrates the multi-model method with an EKF approach can be designed according to different error models to give the optimal estimation for the state. The key parameters of error models can be used to determine the corresponding filter. The Adams explicit formula which has an advantage of high precision prediction is simultaneously fused into the above filter to achieve the much more improvement in attitudes estimation accuracy. The proposed algorithm has been proved through theory analyses and has been tested by both vehicle experiments and lake trials. Results show that the proposed method has better accuracy and effectiveness in terms of attitudes estimation compared with other methods mentioned in the paper for inertial navigation applied to underwater gliders. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

The Effect of Single or Repeated Home Visits on the Hanging and Use of Insecticide-Treated Mosquito Nets following a Mass Distribution Campaign - A Cluster Randomized, Controlled Trial

PubMed Central

Kilian, Albert; Balayo, Connie; Feldman, Mitra; Koenker, Hannah; Lokko, Kojo; Ashton, Ruth A.; Bruce, Jane; Lynch, Matthew; Boulay, Marc

2015-01-01

Background Study objective was to evaluate the effectiveness of commonly used post-campaign hang-up visits on the hanging and use of campaign nets. Methods A cluster-randomized trial was carried out in Uganda following an ITN distribution campaign. Five clusters (parishes, consisting of ∼11 villages each) were randomly selected for each of the three study arms with between 7,534 and 9,401 households per arm. Arm 1 received one hang-up visit, while Arm 2 received two visits by volunteers four and seven months after the campaign. Visits consisted of assistance hanging the net and education on net use. The control arm was only exposed to messages during the campaign itself. Three cross-sectional surveys with a two-stage cluster sampling design, representative of the study populations, were carried out to capture the two key outcome variables of net hanging and ITN use. Sample size was calculated to detect at least a 15 percentage-points change in net use, and was 1811 at endline. The analysis used an intention-to-treat approach. Findings Both hanging and use of ITN increased during follow-up in a similar way in all three study arms. The proportion of the population using an ITN the previous night was 64.0% (95% CI 60.8, 67.2), for one additional visit, 68.2% (63.8, 72.2) for two visits and 64.0% (59.4, 68.5) for the control. The proportion of households with all campaign nets hanging increased from 55.7% to 72.5% at endline (p<0.0005 for trend), with no difference between study arms. Financial cost per household visited was estimated as USD 2.33 for the first visit and USD 2.24 for the second. Conclusions Behavior change communication provided during the campaign or through other channels was sufficient to induce high levels of net hanging and use and additional “hang-up” activities were not cost-effective. PMID:25774676

Measurement of the Mass of an Object Hanging from a Spring--Revisited

ERIC Educational Resources Information Center

Serafin, Kamil; Oracz, Joanna; Grzybowski, Marcin; Koperski, Maciej; Sznajder, Pawel; Zinkiewicz, Lukasz; Wasylczyk, Piotr

2012-01-01

In an open competition, students were to determine the mass of a metal cylinder hanging on a spring inside a transparent enclosure. With the time for experiments limited to 24 h due to the unexpectedly large number of participants, a few surprisingly accurate results were submitted, the best of them differing by no more than 0.5% from the true…

A general method for the layout of ailerons and elevators of gliders and motorplanes

NASA Technical Reports Server (NTRS)

Hiller, M. H.

1979-01-01

A method is described which allows the layout of the spatial driving mechanism of the aileron for a glider or a motorplane to be performed in a systematic manner. In particular, a prescribed input-output behavior of the mechanism can be realized by variation of individual parameters of the spatial four-bar mechanisms which constitute the entire driving mechanism. By means of a sensitivity analysis, a systematic choice of parameters is possible. At the same time the forces acting in the mechanism can be limited by imposing maximum values of the forces as secondary conditions during the variation process.

Intercomparison of numerical simulations, satellite altimetry and glider observations in the Algerian Basin during fall 2014 and 2015: focus on a SARAL/AltiKa track

NASA Astrophysics Data System (ADS)

Aulicino, Giuseppe; Cotroneo, Yuri; Ruiz, Simon; Sanchez Roman, Antonio; Pascual, Ananda; Fusco, Giannetta; Tintoré, Joaquin; Budillon, Giorgio

2017-04-01

The Algerian Basin is a key-place for the study of the general circulation of the Western Mediterranean Sea and its role in reaction to climate change. The presence of both fresh Atlantic waters and more saline resident Mediterranean ones characterizes the basin with an intense inflow/outflow regime and complex circulation patterns. Very energetic mesoscale structures, that evolve from meander of the Algerian Current to isolated cyclonic and anti-cyclonic eddies, dominate the area with marked repercussions on the biological activity. Despite their remarkable importance, this region and its variability are still poorly known and basin-wide high resolution knowledge of its mesoscale and sub-mesoscale features is still incomplete. The monitoring of such complex processes requires a synergic approach that involves integrated observing systems. In recent years, several studies proved the advantages of the combined use of autonomous underwater vehicles, such as gliders, with a new generation of satellite altimeters. In this context, we present the first results of a new integrated oceanographic observing system built up in the Algerian Basin during fall 2014 and 2015, aiming at advancing our knowledge on its main features. The study was realized through the analysis of glider high resolutions three-dimensional observations, collected along the Algerian BAsin Circulation Unmanned Survey (ABACUS) monitoring line, in synergy with co-located SARAL/AltiKa altimetric products and CMEMS numerical simulations. The achieved results confirm that glider derived dynamic height and SARAL/AltiKa absolute dynamic topography present similar patterns, with RMS of the differences ranging between 1.11 and 2.90 cm. Generally, the maximum discrepancies are located nearby the Balearic Islands and the Algerian Coast, but it is important to remark that the correlation coefficients seem to mostly depend on the synopticity between in situ and satellite measurements. Still, this study confirm that

Features of the rupture of free hanging liquid film under the action of a thermal load

NASA Astrophysics Data System (ADS)

Ovcharova, Alla S.

2011-10-01

We consider a deformation and a rupture of a thin liquid film which is hanging between two solid flat walls under the action of concentrated thermal load action. A two-dimensional model is applied to describe the motion of thin layers of viscous non-isothermal liquid under micro-gravity conditions. For flow simulation, two-dimensional Navier-Stokes equations are used. A computational analysis of the influence of thermal loads on the deformation and the rupture behavior of the thin freely hanging film is carried out. It is shown that the rupture of the thin film with generation of a droplet can occur under the thermal beam of specific width acting on the free surface of the film. The results of the model problem solutions are presented.

Design, construction, and testing of a high altitude research glider

NASA Astrophysics Data System (ADS)

Parker, Trevor Llewellyn

Micro aerial vehicle development and atmospheric flight on Mars are areas that require research in very low Reynolds number flight. Facilities for studying these problems are not widely available. The upper atmosphere of the Earth, approximately 100,000 feet AGL, is readily available and closely resembles the atmosphere on Mars, in both temperature and density. This low density also allows normal size test geometry with a very low Reynolds number. This solves a problem in micro aerial vehicle development; it can be very difficult to manufacture instrumented test apparatus in the small sizes required for conventional testing. This thesis documents the design, construction, and testing of a glider designed to be released from a weather balloon at 100,000 feet AGL and operate in this environment, collecting airfoil and aircraft performance data. The challenges of designing a vehicle to operate in a low Reynolds number, low temperature environment are addressed.

Media additives to promote spheroid circularity and compactness in hanging drop platform.

PubMed

Leung, Brendan M; Lesher-Perez, Sasha Cai; Matsuoka, Toshiki; Moraes, Christopher; Takayama, Shuichi

2015-02-01

Three-dimensional spheroid cultures have become increasingly popular as drug screening platforms, especially with the advent of different high throughput spheroid forming technologies. However, comparing drug efficacy across different cell types in spheroid culture can be difficult due to variations in spheroid morphologies and transport characteristics. Improving the reproducibility of compact, circular spheroids contributes to standardizing and increasing the fidelity of the desired gradient profiles in these drug screening three-dimensional tissue cultures. In this study we discuss the role that circularity and compaction has on spheroids, and demonstrate the impact methylcellulose (MethoCel) and collagen additives in the culture media can contribute to more compact and circular spheroid morphology. We demonstrate that improved spheroid formation is not a simple function of increased viscosity of the different macromolecule additives, suggesting that other macromolecular characteristics contribute to improved spheroid formation. Of the various macromolecular additives tested for hanging drop culture, MethoCel provided the most desirable spheroid formation. Additionally, the higher viscosity of MethoCel-containing media improved the ease of imaging of cellular spheroids within hanging drop cultures by reducing motion-induced image blur.

Colony, hanging drop, and methylcellulose three dimensional hypoxic growth optimization of renal cell carcinoma cell lines.

PubMed

Matak, Damian; Brodaczewska, Klaudia K; Lipiec, Monika; Szymanski, Łukasz; Szczylik, Cezary; Czarnecka, Anna M

2017-08-01

Renal cell carcinoma (RCC) is the most lethal of the common urologic malignancies, comprising 3% of all human neoplasms, and the incidence of kidney cancer is rising annually. We need new approaches to target tumor cells that are resistant to current therapies and that give rise to recurrence and treatment failure. In this study, we focused on low oxygen tension and three-dimensional (3D) cell culture incorporation to develop a new RCC growth model. We used the hanging drop and colony formation methods, which are common in 3D culture, as well as a unique methylcellulose (MC) method. For the experiments, we used human primary RCC cell lines, metastatic RCC cell lines, human kidney cancer stem cells, and human healthy epithelial cells. In the hanging drop assay, we verified the potential of various cell lines to create solid aggregates in hypoxic and normoxic conditions. With the semi-soft agar method, we also determined the ability of various cell lines to create colonies under different oxygen conditions. Different cell behavior observed in the MC method versus the hanging drop and colony formation assays suggests that these three assays may be useful to test various cell properties. However, MC seems to be a particularly valuable alternative for 3D cell culture, as its higher efficiency of aggregate formation and serum independency are of interest in different areas of cancer biology.

Hanging drop crystal growth apparatus

NASA Technical Reports Server (NTRS)

Naumann, Robert J. (Inventor); Witherow, William K. (Inventor); Carter, Daniel C. (Inventor); Bugg, Charles E. (Inventor); Suddath, Fred L. (Inventor)

1990-01-01

This invention relates generally to control systems for controlling crystal growth, and more particularly to such a system which uses a beam of light refracted by the fluid in which crystals are growing to detect concentration of solutes in the liquid. In a hanging drop apparatus, a laser beam is directed onto drop which refracts the laser light into primary and secondary bows, respectively, which in turn fall upon linear diode detector arrays. As concentration of solutes in drop increases due to solvent removal, these bows move farther apart on the arrays, with the relative separation being detected by arrays and used by a computer to adjust solvent vapor transport from the drop. A forward scattering detector is used to detect crystal nucleation in drop, and a humidity detector is used, in one embodiment, to detect relative humidity in the enclosure wherein drop is suspended. The novelty of this invention lies in utilizing angular variance of light refracted from drop to infer, by a computer algorithm, concentration of solutes therein. Additional novelty is believed to lie in using a forward scattering detector to detect nucleating crystallites in drop.

Enhanced differentiation of human embryonic stem cells into cardiomyocytes by combining hanging drop culture and 5-azacytidine treatment.

PubMed

Yoon, Byung Sun; Yoo, Seung Jun; Lee, Jeoung Eun; You, Seungkwon; Lee, Hoon Taek; Yoon, Hyun Soo

2006-04-01

Cell replacement therapy is a promising approach for the treatment of cardiac diseases. It is, however, challenged by a limited supply of appropriate cells. Therefore, we have investigated whether functional cardiomyocytes can be efficiently generated from human embryonic stem cells (hESCs). In this study, we developed an efficient protocol for the generation of functional cardiomyocytes from hESCs by combining hanging drop culture and 5-azacytidine, a well-known demethylating agent, and then evaluated the expression of cardiac-specific markers. hESCs were cultured both in the medium without or with 0.1, 1, or 10 microM of 5-azacytidine under a hanging drop culture. The expression of several cardiac-specific markers was determined by real-time PCR, RT-PCR, immunofluorescence, and confocal microscopy. To verify the structural and functional properties of hESC-derived cardiomyocytes, we performed electron microscopy and electrophysiological recording. The efficiency of beating cell generation was significantly improved in the hanging drop culture compared with that in suspension culture. Treatment of hESCs with 0.1 microM of 5-azacytidine for 1-3 days significantly increased the number of beating cells and simultaneously enhanced the expression of cardiac-specific markers. Transmission electron microscopy and electrophysiological recording showed that hESC-derived cardiomyocytes acquired structural and functional properties of cardiomyocytes. In conclusion, these results suggest that differentiation of hESCs into cardiomyocytes can be enhanced by the combination of hanging drop culture and 5-azacytidine treatment. Also the methylation status of genes related to cardiomyocyte development may play an important role in the differentiation of hESCs into cardiomyocytes.

DLR HABLEG- High Altitude Balloon Launched Experimental Glider

NASA Astrophysics Data System (ADS)

Wlach, S.; Schwarzbauch, M.; Laiacker, M.

2015-09-01

The group Flying Robots at the DLR Institute of Robotics and Mechatronics in Oberpfaffenhofen conducts research on solar powered high altitude aircrafts. Due to the high altitude and the almost infinite mission duration, these platforms are also denoted as High Altitude Pseudo-Satellites (HAPS). This paper highlights some aspects of the design, building, integration and testing of a flying experimental platform for high altitudes. This unmanned aircraft, with a wingspan of 3 m and a mass of less than 10 kg, is meant to be launched as a glider from a high altitude balloon in 20 km altitude and shall investigate technologies for future large HAPS platforms. The aerodynamic requirements for high altitude flight included the development of a launch method allowing for a safe transition to horizontal flight from free-fall with low control authority. Due to the harsh environmental conditions in the stratosphere, the integration of electronic components in the airframe is a major effort. For regulatory reasons a reliable and situation dependent flight termination system had to be implemented. In May 2015 a flight campaign was conducted. The mission was a full success demonstrating that stratospheric research flights are feasible with rather small aircrafts.

Robust post-stall perching with a simple fixed-wing glider using LQR-Trees.

PubMed

Moore, Joseph; Cory, Rick; Tedrake, Russ

2014-06-01

Birds routinely execute post-stall maneuvers with a speed and precision far beyond the capabilities of our best aircraft control systems. One remarkable example is a bird exploiting post-stall pressure drag in order to rapidly decelerate to land on a perch. Stall is typically associated with a loss of control authority, and it is tempting to attribute this agility of birds to the intricate morphology of the wings and tail, to their precision sensing apparatus, or their ability to perform thrust vectoring. Here we ask whether an extremely simple fixed-wing glider (no propeller) with only a single actuator in the tail is capable of landing precisely on a perch from a large range of initial conditions. To answer this question, we focus on the design of the flight control system; building upon previous work which used linear feedback control design based on quadratic regulators (LQR), we develop nonlinear feedback control based on nonlinear model-predictive control and 'LQR-Trees'. Through simulation using a flat-plate model of the glider, we find that both nonlinear methods are capable of achieving an accurate bird-like perching maneuver from a large range of initial conditions; the 'LQR-Trees' algorithm is particularly useful due to its low computational burden at runtime and its inherent performance guarantees. With this in mind, we then implement the 'LQR-Trees' algorithm on real hardware and demonstrate a 95 percent perching success rate over 147 flights for a wide range of initial speeds. These results suggest that, at least in the absence of significant disturbances like wind gusts, complex wing morphology and sensing are not strictly required to achieve accurate and robust perching even in the post-stall flow regime.

The effect of weight and drag on the sinking speed and lift/drag ratio of gliders

NASA Technical Reports Server (NTRS)

Kosin, R

1934-01-01

The most important factors in evaluating performance of gliders are minimum sinking speed and minimum gliding angle. To assure their optimum value the energy necessary for flight, that is, the energy of lift and friction must be kept very low, or in other words, weight and total drag which have a decisive effect on the sinking speed and on the gliding angle, must be kept to a minimum. How great the effect of a reduction of these two quantities will be shown in the following.

Hanging angles of two electrostatically repelling pith balls of different masses

NASA Astrophysics Data System (ADS)

Tran, Phuc G.; Mungan, Carl E.

2011-09-01

An analytic solution can be derived for the angles of two mutually repelling charged pith balls of unequal mass hanging from strings from a common point of attachment. Just as in the equal-mass case, a cubic equation is found for the square of the sine of either angle, and an approximation can be used to avoid Cardano's formula for small angles. These results extend a standard problem treated in introductory undergraduate courses in electricity and magnetism.

3D Hanging Drop Culture to Establish Prostate Cancer Organoids.

PubMed

Eder, Theresa; Eder, Iris E

2017-01-01

Three-dimensional (3D) cell culture enables the growth of cells in a multidimensional and multicellular manner compared to conventional cell culture techniques. Especially in prostate cancer research there is a big need for more tissue-recapitulating models to get a better understanding of the mechanisms driving prostate cancer as well as to screen for more efficient drugs that can be used for treatment. In this chapter we describe a 3D hanging drop system that can be used to culture prostate cancer organoids as tumor epithelial monocultures and as epithelial-stromal cocultures.

Optimizing Observation Networks Combining Ships of Opportunity, Gliders, Moored Buoys and FerryBox in the Bay of Biscay and English Channel

NASA Astrophysics Data System (ADS)

Charria, G.; Lamouroux, J.; De Mey, P. J.; Raynaud, S.; Heyraud, C.; Craneguy, P.; Dumas, F.; Le Henaff, M.

2016-02-01

Designing optimal observation networks in coastal oceans remains one of the major challenges towards the implementation of future Integrated Ocean Observing Systems to monitor the coastal environment. In the Bay of Biscay and the English Channel, the diversity of involved processes requires to adapt observing systems to the specific targeted environments. Also important is the requirement for those systems to sustain coastal applications. An efficient way to measure the hydrological content of the water column over the continental shelf is to consider ships of opportunity. In the French observation strategy, the RECOPESCA program, as a component of the High frequency Observation network for the environment in coastal SEAs (HOSEA), aims to collect environmental observations from sensors attached to fishing nets. In the present study, we assess that network performances using the ArM method (Le Hénaff et al., 2009). A reference network, based on fishing vessels observations in 2008, is assessed using that method. Moreover, three scenarios, based on the reference network, a denser network in 2010 and a fictive network aggregated from a pluri-annual collection of profiles, are also analyzed. Two other observational network design experiments have been implemented for the spring season in two regions: 1) the Loire River plume (northern part of the Bay of Biscay) to explore different possible glider endurance lines combined with a fixed mooring to monitor temperature and salinity and 2) the Western English Channel using a glider below FerryBox measurements. These experiments combining existing and future observing systems, as well as numerical ensemble simulations, highlight the key issue of monitoring the whole water column in and close to river plumes (e.g. using gliders), the efficiency of the surface high frequency sampling from FerryBoxes in macrotidal regions and the importance of sampling key regions instead of increasing the number of Voluntary Observing Ships.

Experimental Investigation of a Hypersonic Glider Configuration at a Mach Number of 6 and at Full-Scale Reynolds Numbers

NASA Technical Reports Server (NTRS)

Seiff, Alvin; Wilkins, Max E.

1961-01-01

The aerodynamic characteristics of a hypersonic glider configuration, consisting of a slender ogive cylinder with three highly swept wings, spaced 120 apart, with the wing chord equal to the body length, were investigated experimentally at a Mach number of 6 and at Reynolds numbers from 6 to 16 million. The objectives were to evaluate the theoretical procedures which had been used to estimate the performance of the glider, and also to evaluate the characteristics of the glider itself. A principal question concerned the viscous drag at full-scale Reynolds number, there being a large difference between the total drags for laminar and turbulent boundary layers. It was found that the procedures which had been applied for estimating minimum drag, drag due to lift, lift curve slope, and center of pressure were generally accurate within 10 percent. An important exception was the non-linear contribution to the lift coefficient which had been represented by a Newtonian term. Experimentally, the lift curve was nearly linear within the angle-of-attack range up to 10 deg. This error affected the estimated lift-drag ratio. The minimum drag measurements indicated that substantial amounts of turbulent boundary layer were present on all models tested, over a range of surface roughness from 5 microinches maximum to 200 microinches maximum. In fact, the minimum drag coefficients were nearly independent of the surface smoothness and fell between the estimated values for turbulent and laminar boundary layers, but closer to the turbulent value. At the highest test Reynolds numbers and at large angles of attack, there was some indication that the skin friction of the rough models was being increased by the surface roughness. At full-scale Reynolds number, the maximum lift-drag ratio with a leading edge of practical diameter (from the standpoint of leading-edge heating) was 4.0. The configuration was statically and dynamically stable in pitch and yaw, and the center of pressure was less

Diagnosis of Vitality in Skin Wounds in the Ligature Marks Resulting From Suicide Hanging.

PubMed

Legaz Pérez, Isabel; Falcón, Maria; Gimenez, M; Diaz, F Martínez; Pérez-Cárceles, M D; Osuna, E; Nuno-Vieira, D; Luna, A

2017-09-01

Ascertaining the vital origin of skin wounds is one of the most challenging problems in forensic pathology. The forensic literature describes biomarkers and methods for differentiating vital and postmortem wounds, although no clear conclusions have been reached. The aim of this study was to characterize human vital wounds by analyzing the concentrations of metallic ions and the expression of P-selectin and cathepsin D in skin wounds in the ligature marks in a cohort of suicidal hangings for which vitality was previously demonstrated.A total of 71 skin wounds were analyzed within a postmortem interval of 19 to 36 hours. The concentration of Fe, Zn, Mg, and Ca and the expression of P-selectin and cathepsin D were analyzed together and separately. The majority of autopsied suicidal hangings were men (86%) with complete hanging mode (60.7%) in which there was a high frequency of subcutaneous injuries (78.3%). High concentrations of Ca and Mg compared with Fe and Zn were found. Ca and Zn concentrations decreased, and Fe concentration increased with the seriousness of the injury. A high percentage of moderately negative expression of both proteins was correlated with subcutaneous injury and low or medium concentrations of Fe.In conclusion, the joint study of metallic ions and proteins allows to characterize and to differentiate an injured vital wound of noninjured skin, especially when the damage in the tissue affects to the majority of the structures of the skin, but these results will need to be complemented with other biomarkers in time-controlled samples to further help in the differentiation of vital and postmortem wounds.

Hanging colloidal drop: A new photonic crystal synthesis route

NASA Astrophysics Data System (ADS)

Sandu, Ion; Dumitru, Marius; Fleaca, Claudiu Teodor; Dumitrache, Florian

2018-05-01

High-quality photonic crystals (hundreds of micrometres in thickness) were grown by the free evaporation of a colloidal drop consisting of silica and polystyrene nanospheres with dimensions of 300 nm, 500 nm, and 1000 nm. The essence of experimental findings is that the drop has to hang on a pillar. This leads to the inhibition of the droplet spreading, the minimisation of the convective force, and the zeroing of the static frictional force between nanospheres and the liquid/air interface, where the first layer is formed. The theoretical essence is the continuous adjustment of nanospheres positions during the growth of photonic crystal, a key condition of the self-assembling phenomenon.

[Forensic medical peculiarities of mechanical strangulation asphyxia in the hanged subjects aged above 50 years].

PubMed

Chertovskikh, A A; Tuchik, E S; Astashkina, O G

2014-01-01

This paper presents the qualitative and quantitative characteristics of the morphological manifestations of mechanical strangulation asphyxia in the hanged subjects aged 50 years and the elder ones. In addition, the new diagnostic approaches to the expert confirmation of this condition are proposed.

Effects of an aft facing step on the surface of a laminar flow glider wing

NASA Technical Reports Server (NTRS)

Sandlin, Doral R.; Saiki, Neal

1993-01-01

A motor glider was used to perform a flight test study on the effects of aft facing steps in a laminar boundary layer. This study focuses on two dimensional aft facing steps oriented spanwise to the flow. The size and location of the aft facing steps were varied in order to determine the critical size that will force premature transition. Transition over a step was found to be primarily a function of Reynolds number based on step height. Both of the step height Reynolds numbers for premature and full transition were determined. A hot film anemometry system was used to detect transition.

Behavioral and endocrinological correlates of social status in the male sugar glider (Petaurus breviceps Marsupialia: Petauridae).

PubMed

Mallick, J; Stoddart, D M; Jones, I; Bradley, A J

1994-06-01

Socially dominant male sugar gliders are heavier than socially subordinate males, have higher plasma testosterone and lower cortisol concentrations, win more social encounters, scan the arena more, scent-mark more, and are more active and move more quickly, even though they spend more time in the colony nesting box. When they are transferred into a foreign stable colony there is an impressive reversal of behavioral measures and a concomitant decrease in concentration of plasma testosterone and rise in cortisol that is apparent over the first 3 weeks of observation.

Redifferentiation of in vitro expanded adult articular chondrocytes by combining the hanging-drop cultivation method with hypoxic environment.

PubMed

Martinez, Inigo; Elvenes, Jan; Olsen, Randi; Bertheussen, Kjell; Johansen, Oddmund

2008-01-01

The main purpose of this work has been to establish a new culturing technique to improve the chondrogenic commitment of isolated adult human chondrocytes, with the aim of being used during cell-based therapies or tissue engineering strategies. By using a rather novel technique to generate scaffold-free three-dimensional (3D) structures from in vitro expanded chondrocytes, we have explored the effects of different culture environments on cartilage formation. Three-dimensional chondrospheroids were developed by applying the hanging-drop technique. Cartilage tissue formation was attempted after combining critical factors such as serum-containing or serum-free media and atmospheric (20%) or low (2.5%) oxygen tensions. The quality of the formed microtissues was analyzed by histology, immunohistochemistry, electron microscopy, and real-time PCR, and directly compared with native adult cartilage. Our results revealed highly organized, 3D tissue-like structures developed by the hanging-drop method. All culture conditions allowed formation of 3D spheroids; however, cartilage generated under low oxygen tension had a bigger size, enhanced matrix deposition, and higher quality of cartilage formation. Real-time PCR demonstrated enhanced expression of cartilage-specific genes such us collagen type II and aggrecan in 3D cultures when compared to monolayers. Cartilage-specific matrix proteins and genes expressed in hanging-drop-developed spheroids were comparable to the expression obtained by applying the pellet culture system. In summary, our results indicate that a combination of 3D cultures of chondrocytes in hanging drops and a low oxygen environment represent an easy and convenient way to generate cartilage-like microstructures. We also show that a new specially tailored serum-free medium is suitable for in vitro cartilage tissue formation. This new methodology opens up the possibility of using autogenously produced solid 3D structures with redifferentiated chondrocytes as an

Extending total parenteral nutrition hang time in the neonatal intensive care unit: is it safe and cost effective?

PubMed

Balegar V, Kiran Kumar; Azeem, Mohammad Irfan; Spence, Kaye; Badawi, Nadia

2013-01-01

To investigate the effects of prolonging hang time of total parenteral nutrition (TPN) fluid on central line-associated blood stream infection (CLABSI), TPN-related cost and nursing workload. A before-after observational study comparing the practice of hanging TPN bags for 48 h (6 February 2009-5 February 2010) versus 24 h (6 February 2008-5 February 2009) in a tertiary neonatal intensive care unit was conducted. The main outcome measures were CLABSI, TPN-related expenses and nursing workload. One hundred thirty-six infants received 24-h TPN bags and 124 received 48-h TPN bags. Median (inter-quartile range) gestation (37 weeks (33,39) vs. 36 weeks (33,39)), mean (±standard deviation) admission weight of 2442 g (±101) versus 2476 g (±104) and TPN duration (9.7 days (±12.7) vs. 9.9 days (±13.4)) were similar (P > 0.05) between the 24- and 48-h TPN groups. There was no increase in CLABSI with longer hang time (0.8 vs. 0.4 per 1000 line days in the 24-h vs. 48-h group; P < 0.05). Annual cost saving using 48-h TPN was AUD 97,603.00. By using 48-h TPN, 68.3% of nurses indicated that their workload decreased and 80.5% indicated that time spent changing TPN reduced. Extending TPN hang time from 24 to 48 h did not alter CLABSI rate and was associated with a reduced TPN-related cost and perceived nursing workload. Larger randomised controlled trials are needed to more clearly delineate these effects. © 2012 The Authors. Journal of Paediatrics and Child Health © 2012 Paediatrics and Child Health Division (Royal Australasian College of Physicians).

Sizing of colloidal particle and protein molecules in a hanging fluid drop

NASA Technical Reports Server (NTRS)

Ansari, Rafat R.; Suh, Kwang I.

1995-01-01

We report non-invasive particle size measurements of polystyrene latex colloidal particles and bovine serum albumin (BSA) protein molecules suspended in tiny hanging fluid drops of 30 micro-Liter volume using a newly designed fiber optic probe. The probe is based upon the principles of the technique of dynamic light scattering (DLS). The motivation for this work comes from growing protein crystals in outer space. Protein crystals have been grown previously in hanging drops in microgravity experiments on-board the space shuttle orbiter. However, obtaining quantitative information on nucleation and growth of the protein crystals in real time has always been a desired goal, but hitherto not achieved. Several protein researchers have shown interest in using DLS to monitor crystal growth process in a droplet, but elaborate instrumentation and optical alignment problems have made in-situ applications difficult. We demonstrate that such an experiment is now possible. Our system offers fast (5 seconds) determination of particle size, utilize safe levels of very low laser power (less than or equal to 0.2 mW), a small scattering volume (approximately 2 x 10(exp -5) cu mm) and high spatial coherence (Beta) values. This is a major step forward when compared to currently available DLS systems.

Liver hanging maneuver for right hemiliver in situ donation – anatomical considerations

PubMed Central

Gadžijev, E.M.; Ravnik, D.; Hribernik, M.

2006-01-01

Background. An anatomical study was carried out to evaluate the safety of the liver hanging maneuver for the right hemiliver in living donor and in situ splitting transplantation. During this procedure a 4–6 cm blind dissection is performed between the inferior vena cava and the liver. Short subhepatic veins entering the inferior vena cava from segments 1 and 9 could be torn with consequent hemorrhage. Materials and methods. One hundred corrosive casts of livers were evaluated to establish the position and diameter of short subhepatic veins and the inferior right hepatic vein. Results. The average distance from the right border of the inferior vena cava to the opening of segment 1 veins was 16.7±3.4 mm and to the entrance of segment 9 veins was 5.0±0.5 mm. The width of the narrowest point on the route of blind dissection was determined, with the average value being 8.7±2.3 mm (range 2–15 mm). Discussion. The results show that the liver hanging maneuver is a safe procedure. A proposed route of dissection minimizes the risk of disrupting short subhepatic veins (7%). PMID:18333236

Hanging Maneuver for Stomach Traction in Laparoscopic Distal Pancreatic Resections: An Original Technique Applied in 218 Patients.

PubMed

Dokmak, Safi; Aussilhou, Béatrice; Ftériche, Fadhel Samir; Belghiti, Jacques; Sauvanet, Alain

2017-01-01

Stomach traction done to expose the pancreas is still a problem in laparoscopic left pancreatic resections. We developed a simple hanging maneuver to retract the stomach rapidly and effectively. After dividing the gastrocolic ligament, the stomach was encircled with a tape, turned along its horizontal axis and pulled with an epigastric trocar, which was later removed. This technique was used in all patients who underwent laparoscopic left pancreatic resections including 165 distal pancreatectomies (DP), 35 central pancreatectomies (CP) and 18 enucleations (En). Demographics, surgical and postoperative outcome data were recorded. There were no mortalities. The mean operative time for DP, CP and En were 174, 191 and 104 min, respectively. The transfusion (0-4%) and conversion (0-3%) rates were low for all procedures. Morbidity was mainly represented by pancreatic fistula and grades (B + C) for DP, CP and En were observed in 26, 22 and 17%, respectively. No complication related to hanging of the stomach, like gastric perforation, was observed. Re-intervention and the mean hospital stay for DP, CP and En were observed in 5, 11 and 0% and were 16, 22 and 12, respectively. The readmission rate was low (0-9%). Hanging maneuver of the stomach is a simple procedure to rapidly, safely and effectively retract the stomach during left laparoscopic pancreatic resections. © 2016 S. Karger AG, Basel.

Detrended cross-correlation analysis on RMB exchange rate and Hang Seng China Enterprises Index

NASA Astrophysics Data System (ADS)

Ruan, Qingsong; Yang, Bingchan; Ma, Guofeng

2017-02-01

In this paper, we investigate the cross-correlations between the Hang Seng China Enterprises Index and RMB exchange markets on the basis of a cross-correlation statistic test and multifractal detrended cross-correlation analysis (MF-DCCA). MF-DCCA has, at best, serious limitations for most of the signals describing complex natural processes and often indicates multifractal cross-correlations when there are none. In order to prevent these false multifractal cross-correlations, we apply MFCCA to verify the cross-correlations. Qualitatively, we find that the return series of the Hang Seng China Enterprises Index and RMB exchange markets were, overall, significantly cross-correlated based on the statistical analysis. Quantitatively, we find that the cross-correlations between the stock index and RMB exchange markets were strongly multifractal, and the multifractal degree of the onshore RMB exchange markets was somewhat larger than the offshore RMB exchange markets. Moreover, we use the absolute return series to investigate and confirm the fact of multifractality. The results from the rolling windows show that the short-term cross-correlations between volatility series remain high.

Flutter instability of freely hanging articulated pipes conveying fluid

NASA Astrophysics Data System (ADS)

Schouveiler, Lionel; Chermette, Félix

2018-03-01

We experimentally investigate the stability of freely hanging articulated pipes made of rigid segments connected by flexible joints and with their displacements constrained in a vertical plane. When the velocity of the fluid conveyed by the pipe is increased, flutter-type instability occurs above a critical value. The critical velocity and the characteristics of the flutter modes (frequency, amplitude, and shape) are determined as a function of the number n of segments into the pipe which is varied from 2 to 5. Experimental results are compared to predictions from linear stability analysis extending previous studies by taking into account damping due to the dissipation in the joints. Qualitative agreement is found and the limits of the analysis are discussed.

Hanging out at the airport: Unusual upside-down perching behavior by Eurasian Jackdaws (Corvus monedula) in a human-dominated environment

USGS Publications Warehouse

Katzner, Todd E.

2016-01-01

Animals occupying human-dominated environments show the capacity for behavioral flexibility. Corvids are among the most intelligent synanthropic bird species. During a layover at Schipol Airport in Amsterdam, Netherlands, I photographically documented Eurasian Jackdaws (Corvus monedula) perching upside down from a building cornice. In contrast to other reports of hanging birds, these jackdaws did not forage or play while upside down and appeared to use the perching spot to observe their surroundings. Although Corvids and Psittacines are known to hang upside down, especially in captive situations, such behaviors are rarely documented in the wild, and never before in association with human-built structures.

Comparison of spring-loaded, loss of resistance and hanging drop techniques in lumbar epidural blocks.

PubMed

Gülen, Güven; Akkaya, Taylan; Ozkan, Derya; Kaydul, Mehmet; Gözaydin, Orhan; Gümüş, Haluk

2012-01-01

The spring-loaded syringe is a loss of resistance syringe that provide a more objective sign that the epidural space has been entered compared with the traditional techniques. The aim of this study was to compare the time required to locate the epidural space and the backache incidence with the spring-loaded (SL), loss of resistance (LOR) and the hanging drop (HD) techniques for epidural blocks in patients undergoing transurethral resection procedure. Sixty patients undergoing transurethral resections were enrolled in the study. The patients were randomly assigned to one of three groups. Epidural block was performed in the first group with a spring-loaded syringe (n=20), in the second group with loss-of-resistance syringe (n=20), and in the third group with the hanging drop technique (n=20). The required time to locate the epidural space, the number of attempts, the incidence of dural puncture and the backache incidence were assessed during the procedure and for four weeks after the procedure in all patients. The required time to locate the epidural space was 29.1 ± 9.16 seconds in Group 1; 45.25 ± 19.58 seconds in Group 2, and 47.35 ± 11.42 seconds in Group 3 (p<0.001). In Group 1 this was significantly shorter than the other two groups. There was no significant difference in the number of attempts, the incidence of dural puncture and backache incidence between the three groups (p>0.05). The use of SL syringe was found to have a shorter time period to locate the epidural space when compared with the LOR syringe and hanging drop technique.

Analogue modelling of thrust systems: Passive vs. active hanging wall strain accommodation and sharp vs. smooth fault-ramp geometries

NASA Astrophysics Data System (ADS)

Rosas, F. M.; Duarte, J. C.; Almeida, P.; Schellart, W. P.; Riel, N.; Terrinha, P.

2017-06-01

We present new analogue modelling results of crustal thrust-systems in which a deformable (brittle) hanging wall is assumed to endure passive internal deformation during thrusting, i.e. exclusively as a consequence of having to adapt its shape to the variable geometry of a rigid footwall. Building on previous experimental contributions, we specifically investigate the role of two so far overlooked critical variables: a) concave-convex (CC) vs. flat-ramp-flat (FRF) thrust ramp geometry; and b) presence vs. absence of a basal velocity discontinuity (VD). Regarding the first variable, we compare new results for considered (CC) smoother ramp types against classical experiments in which (FRF) sharp ramp geometries are always prescribed. Our results show that the considered sharp vs. smooth variation in the thrust-ramp geometry produces important differences in the distribution of the local stress field in the deformable hanging wall above both (lower and upper) fault bends, with corresponding styles of strain accommodation being expressed by marked differences in measured morpho-structural parameters. Regarding the second variable, we for the first time report analogue modelling results of this type of experiments in which basal VDs are experimentally prescribed to be absent. Our results critically show that true passive hanging wall deformation is only possible to simulate in the absence of any basal VD, since active shortening accommodation always necessarily occurs in the hanging wall above such a discontinuity (i.e. above the lower fault bend). In addition, we show that the morpho-structural configuration of model thrust-wedges formed for prescribed VD absence conditions complies well with natural examples of major overthrusts, wherein conditions must occur that approximate a frictionless state along the main basal thrust-plane.

Intracellular localization of pregnane X receptor in HepG2 cells cultured by the hanging drop method.

PubMed

Yokobori, Kosuke; Kobayashi, Kaoru; Azuma, Ikuko; Akita, Hidetaka; Chiba, Kan

2017-10-01

Pregnane X receptor (PXR) is localized in the cytoplasm of liver cells, whereas it is localized in the nucleus of monolayer-cultured HepG2 cells. Since cultured cells are affected by the microenvironment in which they are grown, we studied the effect of three-dimensional (3D) culture on the localization of PXR in HepG2 cells using the hanging drop method. The results showed that PXR was retained in the cytoplasm of HepG2 cells and other human hepatocarcinoma cell lines (FLC5, FLC7 and Huh7) when they were cultured by the hanging drop method. Treatment with rifampicin, a ligand of PXR, translocated PXR from the cytoplasm to nucleus and increased expression levels of CYP3A4 mRNA in HepG2 cells cultured by the hanging drop method. These findings suggest that 3D culture is a key factor determining the intracellular localization of PXR in human hepatocarcinoma cells and that PXR that becomes retained in the cytoplasm of HepG2 cells with 3D culture has functions of nuclear translocation and regulation of target genes in response to human PXR ligands. Three-dimensionally cultured hepatocarcinoma cells would be a useful tool to evaluate induction potency of drug candidates and also to study mechanisms of nuclear translocation of PXR by human PXR ligands. Copyright © 2017 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.

Cervical spine injuries in suicidal hanging without a long-drop--patterns and possible underlying mechanisms of injury: an autopsy study.

PubMed

Nikolić, Slobodan; Zivković, Vladimir

2014-06-01

The incidence of cervical spine injuries in suicidal hangings with a short-drop has been reported to be extremely low or non-existent. The aim of this study was to determine the frequency and pattern of cervical spine injuries in suicidal hanging. A retrospective autopsy study was performed and short-drop suicidal hanging cases with documented cervical spine injuries were identified. This group was further analyzed with regard to the gender and age of the deceased, the position of the ligature knot, the presence of hyoid-laryngeal fractures, and the level of cervical spine injury. Cervical spine injuries were present in 25 of the 766 cases, with an average age of 71.9 ± 10.7 years (range 39-88 years). In 16 of these 25 cases, the ligature knot was in the anterior position. The most common pattern of cervical spine injury included partial or complete disruption of the anterior longitudinal ligament and widening of the lower cervical spine disk spaces, associated with absence of hyoid-laryngeal fractures. Cervical spine injuries are not commonly found in short-drop suicidal hanging, occurring in only 3.3 % of all observed cases. Cervical spine injury may be occurring in 80 % of subjects aged 66.5 years and above. The most common pattern of cervical spine injury included anterior longitudinal ligament disruption of the lower cervical spine, disk space widening, and no vertebral body displacement. These injuries were mainly associated with an anterior knot position, and may be a consequence of loop pressure to the posterior neck and cervical spine hyperextension.

Rehabilitation approaches to dysphagia that was developed for a patient who attempted to commit suicide by hanging: a case report.

PubMed

Kii, Y; Mizuma, M

2014-04-01

We report our experience with a case of attempted suicidal hanging presenting with dysphagia, in which rehabilitation approaches resulted in improvement. A 36-year-old man was discovered collapsed at home. From the finding of a broken cord nearby, attempted suicide by hanging was suspected. He was transported to hospital after 40 minutes, and regained consciousness after emergency treatment. There were no noteworthy findings on brain magnetic resonance imaging, cervical spine computed tomography, or vocal cord examination. There were no noteworthy psychiatric disorders or cognitive abnormalities. On hospital day 10, he showed signs of dysphagia. Videofluoroscopic examination of swallowing revealed piriform sinuses residue and aspiration after ingestion of jelly in any posture. Rehabilitation approaches were started based on the diagnosis of dysphagia due to impaired transit through the piriform sinuses. The patient achieved independent oral intake by 40 days after the injury. In this case, dysphagia was considered attributable to compression of the vagus nerves running along the lateral aspects of the neck by the cord used in the hanging. If compression is brief, full functional recovery can be expected. Implementation of rehabilitation approaches is also important in this situation.

Assimilation of glider and mooring data into a coastal ocean model

NASA Astrophysics Data System (ADS)

Jones, Emlyn M.; Oke, Peter R.; Rizwi, Farhan; Murray, Lawrence M.

We have applied an ensemble optimal interpolation (EnOI) data assimilation system to a high resolution coastal ocean model of south-east Tasmania, Australia. The region is characterised by a complex coastline with water masses influenced by riverine input and the interaction between two offshore current systems. Using a large static ensemble to estimate the systems background error covariance, data from a coastal observing network of fixed moorings and a Slocum glider are assimilated into the model at daily intervals. We demonstrate that the EnOI algorithm can successfully correct a biased high resolution coastal model. In areas with dense observations, the assimilation scheme reduces the RMS difference between the model and independent GHRSST observations by 90%, while the domain-wide RMS difference is reduced by a more modest 40%. Our findings show that errors introduced by surface forcing and boundary conditions can be identified and reduced by a relatively sparse observing array using an inexpensive ensemble-based data assimilation system.

The quiet revolution: continuous glider monitoring at ocean 'choke' points as a key component of new cross-platform ocean observation systems

NASA Astrophysics Data System (ADS)

Heslop, E. E.; Tintore, J.; Ruiz, S.; Allen, J.; López-Jurado, J. L.

2014-12-01

A quiet revolution is taking place in ocean observations; in the last decade new multi-platform, integrated ocean observatories have been progressively implemented by forward looking countries with ocean borders of economic and strategic importance. These systems are designed to fill significant gaps in our knowledge of the ocean state and ocean variability, through long-term, science and society-led, ocean monitoring. These ocean observatories are now delivering results, not the headline results of a single issue experiment, but carefully and systematically improving our knowledge of ocean variability, and thereby, increasing model forecast skill and our ability to link physical processes to ecosystem response. Here we present the results from a 3-year quasi-continuous glider monitoring of a key circulation 'choke' point in the Western Mediterranean, undertaken by SOCIB (Balearic Islands Coastal Ocean Observing and Forecasting System). For the first time data from the high frequency glider sampling show variations in the transport volumes of water over timescales of days to weeks, as large as those previously only identifiable as seasonal or eddy driven. Although previous surveys noted high cruise-to-cruise variability, they were insufficient to show that in fact water volumes exchanged through this narrow 'choke' point fluctuate on 'weather' timescales. Using the glider data to leverage an 18-year record of ship missions, we define new seasonal cycles for the exchange of watermasses, challenging generally held assumptions. The pattern of the exchange is further simplified through the characterisation of 5 circulation modes and the defining of a new seasonal cycle for the interplay between mesoscale and basin scale dynamics. Restricted 'choke points' between our ocean basins are critical locations to monitor water transport variability, as they constrain the inter-basin exchange of heat, salt and nutrients. At the Ibiza Channel 'choke' point, the exchange of

Reliability of the Straight-Arm Hang for Testing Muscular Endurance among Children 2 to 5.

ERIC Educational Resources Information Center

Gabbard, Carl; And Others

1979-01-01

The straight-arm hang as a reliable testing instrument for young children is investigated with results indicating that it is a reliable indicator for four- and five-year-old children. Questionable results may occur if utilized for younger subjects and further investigation is recommended for six-to-nine-year-old subjects. (JD)

Community point distribution of insecticide-treated bed nets and community health worker hang-up visits in rural Zambia: a decision-focused evaluation.

PubMed

Wang, Paul; Connor, Alison L; Joudeh, Ammar S; Steinberg, Jeffrey; Ndhlovu, Ketty; Siyolwe, Musanda; Ntebeka, Bristol; Chibuye, Benjamin; Hamainza, Busiku

2016-03-03

In 2013, the Zambian Ministry of Health through its National Malaria Control Programme distributed over two million insecticide-treated bed nets (ITNs) in four provinces using a door-to-door distribution strategy, and more than 6 million ITNs were allocated to be distributed in 2014. This study was commissioned to measure attendance rates at a community point distribution and to examine the impact of follow-up community health worker (CHW) hang-up visits on short and medium-term ITN retention and usage with a view of informing optimal ITN distribution strategy in Zambia. Households received ITNs at community point distributions conducted in three rural communities in Rufunsa District, Zambia. Households were then randomly allocated into five groups to receive CHW visits to hang any unhung ITNs at different intervals: 1-3, 5-7, 10-12, 15-17 days, and no hang-up visit. Follow-up surveys were conducted among all households at 7-11 weeks after distribution and at 5-6 months after distribution to measure short- and medium-term household retention and usage of ITNs. Of the 560 pre-registered households, 540 (96.4 %) attended the community point distribution. Self-installation of ITNs by households increased over the first 10 days after the community point distribution. Retention levels remained high over time with 90.2 % of distributed ITNs still in the household at 7-11 weeks and 85.7 % at 5-6 months. Retention did not differ between households that received a CHW visit and those that did not. At 7-11 weeks, households had an average of 73.8 % of sleeping spaces covered compared to 80.3 % at 5-6 months. On average, 65.6 % of distributed ITNs were hanging at 7-11 weeks compared to 63.1 % at 5-6 months. While a CHW hang-up visit was associated with increased usage at 7-11 weeks, this difference was no longer apparent at 5-6 months. This evaluation revealed that (1) the community point distributions achieved high attendance rates followed by acceptable rates of short

Determination of trace arsenic on hanging copper amalgam drop electrode.

PubMed

Piech, Robert; Baś, Bogusław; Niewiara, Ewa; Kubiak, Władysław W

2007-04-30

Hanging copper amalgam drop electrode has been applied for trace determination of arsenic by cathodic stripping analysis. Detection limit for As(III) as low as 0.33nM (0.02mug/L) at deposition time (240s) could be obtained. For seven successive determinations of As(III) at concentration of 5nM relative standard deviation was 2.5% (n=7). Interferences from selected metals and surfactant substances were examined. Absence of copper ions in sample solution causes easier optimization and makes method less vulnerable on contamination. The developed method was validated by analysis of certified reference materials (CRMs) and applied to arsenic determinations in natural water samples.

Arbitrary-level hanging nodes for adaptive hphp-FEM approximations in 3D

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pavel Kus; Pavel Solin; David Andrs

2014-11-01

In this paper we discuss constrained approximation with arbitrary-level hanging nodes in adaptive higher-order finite element methods (hphp-FEM) for three-dimensional problems. This technique enables using highly irregular meshes, and it greatly simplifies the design of adaptive algorithms as it prevents refinements from propagating recursively through the finite element mesh. The technique makes it possible to design efficient adaptive algorithms for purely hexahedral meshes. We present a detailed mathematical description of the method and illustrate it with numerical examples.

SU-F-T-75: Dosimetry Considerations in the Use of Hanging-Eye Block for Lesions of the Conjunctiva

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grelewicz, Z; Lee, B; Cutright, D

Purpose: Superficial lesions in the conjunctiva are frequently treated with en face electrons, using a hanging block to spare dose to the lens of the eye. Dose to the tumor and lens depend on the design and setup of the block and supporting apparatus. We performed in phantom measurements in order to characterize the dose sparing effects of the block as well as the under-dosing effect under the supporting apparatus for 6 MeV treatment. Methods: The commercial hanging block studied uses a 1.2 cm diameter tungsten cylinder supported by a 3 mm diameter acrylic rod. Point dose measurements under themore » hanging block, under an unblocked part of the field, and under the acrylic rod were performed using MOSFET detectors. In addition, EBT3 film was used for both PDD and profile measurements at a depth in phantom of 3 mm for both 105 and 103 cm SSD. Results: MOSFET measurements reported a dose reduction of 95% under the tungsten block when using an SSD of 103 cm, and 86% when using an SSD of 105 cm at a depth in phantom of 3 mm. Film measurements showed that the area under the acrylic rod may be under-dosed by as much as 30% when using 103 SSD. MOSFET measurements confirmed that when using an SSD of 103 cm, the area under the acrylic rod is under-dosed by up to 30% at 3mm depth, compared to the unblocked part of the field. Conclusion: The effectiveness of the commercial hanging block apparatus depends on setup, with 95% lens sparing possible with an SSD of 103 cm. This short SSD is necessary for sharp penumbra. At this SSD, substantial under-dosing under the acrylic support rod is possible. This must be mitigated with either feathering, or using an alternative method of support for the tungsten block.« less

Structural analysis using thrust-fault hanging-wall sequence diagrams: Ogden duplex, Wasatch Range, Utah

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schirmer, T.W.

1988-05-01

Detailed mapping and cross-section traverses provide the control for structural analysis and geometric modeling of the Ogden duplex, a complex thrust system exposed in the Wasatch Mountains, east of Ogden, Utah. The structures consist of east-dipping folded thrust faults, basement-cored horses, lateral ramps and folds, and tear faults. The sequence of thrusting determined by means of lateral overlap of horses, thrust-splay relationships, and a top-to-bottom piggyback development is Willard thrust, Ogden thrust, Weber thrust, and Taylor thrust. Major decollement zones occur in the Cambrian shales and limestones. The Tintic Quartzite is the marker for determining gross geometries of horses. Thismore » exposed duplex serves as a good model to illustrate the method of constructing a hanging-wall sequence diagram - a series of longitudinal cross sections that move forward in time and space, and show how a thrust system formed as it moved updip over various footwall ramps. A hanging wall sequence diagram also shows the complex lateral variations in a thrust system and helps to locate lateral ramps, lateral folds, tear faults, and other features not shown on dip-oriented cross sections. 8 figures.« less

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array.

PubMed

Tung, Yi-Chung; Hsiao, Amy Y; Allen, Steven G; Torisawa, Yu-suke; Ho, Mitchell; Takayama, Shuichi

2011-02-07

Culture of cells as three-dimensional (3D) aggregates can enhance in vitro tests for basic biological research as well as for therapeutics development. Such 3D culture models, however, are often more complicated, cumbersome, and expensive than two-dimensional (2D) cultures. This paper describes a 384-well format hanging drop culture plate that makes spheroid formation, culture, and subsequent drug testing on the obtained 3D cellular constructs as straightforward to perform and adapt to existing high-throughput screening (HTS) instruments as conventional 2D cultures. Using this platform, we show that drugs with different modes of action produce distinct responses in the physiological 3D cell spheroids compared to conventional 2D cell monolayers. Specifically, the anticancer drug 5-fluorouracil (5-FU) has higher anti-proliferative effects on 2D cultures whereas the hypoxia activated drug commonly referred to as tirapazamine (TPZ) are more effective against 3D cultures. The multiplexed 3D hanging drop culture and testing plate provides an efficient way to obtain biological insights that are often lost in 2D platforms.

Glider and remote sensing observations of the upper ocean response to an extended shallow coastal diversion of wastewater effluent

NASA Astrophysics Data System (ADS)

Seegers, Bridget N.; Teel, Elizabeth N.; Kudela, Raphael M.; Caron, David A.; Jones, Burton H.

2017-02-01

The Orange County Sanitation District (OCSD) diverted wastewater discharge (5.3 × 108 l d-1) from its primary deep (56 m) outfall 8 km offshore, to a secondary shallower (16 m) outfall 1.6 km offshore for a period of three weeks. It was anticipated that the low salinity and density of the effluent would cause it to rise to the surface with limited dilution, elevating nutrient concentrations in near-surface waters and stimulating phytoplankton blooms in the region. Three Teledyne Webb Slocum gliders and a Liquid Robotics surface wave glider were deployed on transects near the outfalls to acquire high spatial and temporal coverage of physical and chemical parameters before, during, and after the wastewater diversion. Combined autonomous underwater vehicle (AUV) and MODIS-Aqua satellite ocean color data indicated that phytoplankton biomass increased in the upper water column in response to the diversion, but that the magnitude of the response was spatially patchy and significantly less than expected. Little evidence of the plume or its effects was detectable 72 h following the diversion. The effluent plume exhibited high rates of dilution and mixed throughout the upper 20 m and occasionally throughout the upper 40 m during the diversion. Rapid plume advection and dilution appeared to contribute to the muted impact of the nutrient-rich effluent on the phytoplankton community in this coastal ecosystem.

Knickpoints and Hanging Valleys of Licus Vallis, Mars

NASA Astrophysics Data System (ADS)

Goudge, T. A.; Fassett, C.

2016-12-01

Licus Vallis is a 350 km long valley system located along the dichotomy boundary on Mars. The main trunk of the valley is incised 200-700 m into the surrounding terrain. The valley heads at an outlet breach of a shallow, 30 km diameter impact crater, and is also fed by a system of tributaries incised into the plateau surrounding Licus Vallis. Many of the tributary valleys, as well as the main stem of the valley fed by the paleolake outlet, have profiles that are not smoothly graded, but rather have distinct reaches with concave downward topography. These sections are either knickpoints or hanging valleys that develop in response to changes in the effective local base level, changes in climate conditions during incision of the valley, or lithologic boundaries in the substrate. Here we present remote sensing observations from images and topography to test these competing hypotheses and further characterize the evolution of this large valley system. Slope-watershed area relationships for the tributaries and main trunk valley are used to distinguish between knickpoints and hanging valleys. Analysis of orbital images does not reveal any distinct layer above which knickpoints develop, and the elevation of knickpoints show no systematic trends that might be expected of a regional lithologic unit(s). Our preliminary results suggest that the distance of knickpoint retreat is correlated with the position of the tributary valley and not the watershed area. Downstream valleys have retreated the most, suggesting they have had the most time to adjust to lowering of the local base level associated with incision of the main valley. These results are most consistent with a wave of incision sweeping up the valley system as it adjusts to a low base level in the northern plains. This conclusion is also consistent with observations of the incision depth of Licus Vallis, which increases approximately linearly downstream. Understanding this signature of base level control on the incision

Energy stability of droplets and dry spots in a thin film model of hanging drops

NASA Astrophysics Data System (ADS)

Cheung, Ka-Luen; Chou, Kai-Seng

2017-10-01

The 2-D thin film equation describing the evolution of hang drops is studied. All radially symmetric steady states are classified, and their energy stability is determined. It is shown that the droplet with zero contact angle is the only global energy minimizer and the dry spot with zero contact angle is a strict local energy minimizer.

Animations, games, and virtual reality for the Jing-Hang Grand Canal.

PubMed

Chen, Wenzhi; Zhang, Mingmin; Pan, Zhigeng; Liu, Gengdai; Shen, Huaqing; Chen, Shengnan; Liu, Yong

2010-01-01

Digital heritage, an effective method to preserve and present natural and cultural heritage, is engaging many heritage preservation specialists and computer scientists. In particular, computer graphics researchers have become involved, and digital heritage has employed many CG techniques. For example, Daniel Pletinckx and his colleagues employed VR in a real museum at Ename, Belgium, and Zhigeng Pan and his colleagues applied it to construct a virtual Olympics museum. Soo-Chang Pei and his colleagues focused on restoring ancient Chinese paintings. Here, we describe how we've applied animations, computer games, and VR to China's famous Jing-Hang Grand Canal.

Characterization With Scanning Electron Microscopy/Energy-Dispersive X-ray Spectrometry of Microtraces From the Ligature Mean in Hanging Mechanical Asphyxia: A Series of Forensic Cases.

PubMed

Maghin, Francesca; Andreola, Salvatore Ambrogio; Boracchi, Michele; Gentile, Guendalina; Maciocco, Francesca; Zoja, Riccardo

2018-03-01

The authors applied scanning electron microscopy with energy-dispersive x-ray spectrometry to the furrow derived from hanging means. The study was conducted with the purpose to detect possible extraneous microtraces, deriving from the ligature, that could have had an interaction with the cutaneous biological matrix, thanks to a transfert mechanism, in the proximities of the lesion.Fifteen cutaneous samples of the furrow and an equal number of fragments of graphite tape, directly positioned on the lesion produced by the ligature mean and used as a "conductor" of possible traces, were analyzed using scanning electron microscopy with energy-dispersive x-ray spectrometry.The research of microscopic traces on the furrow using this technique highlights extraneous traces leading to 3 main categories: natural fabrics, and synthetic and metallic materials, excluding possible environmental pollutants. The analysis, run on 7 hanging deaths, made available by the judicial authority, found a morphological and compositional compatibility with the traces found on the cutaneous furrow produced during hanging.The technique used in this study is innovative in the pathological-forensic field, and can be considered useful in clarifying and studying this typology of asphyxia leading to a specific ligature material, when missing, or attributing the cause of death to hanging when the furrow is not macroscopically obvious.

Ingestion of safety razor blade and delayed hanging in a complex suicide.

PubMed

Chauhan, Mohit Singh; Behera, C; Naagar, Sunil; Sreenivas, M

2016-12-01

Ingestion of a foreign body is mostly accidental in children and intentional in prisoners to achieve hospitalization; however, use of this method of suicide is rare. We report a case where the victim first ingested a safety razor blade, but failed to die and then hanged himself, but failed again and finally succumbed to the complications on the sixth day. He had also attempted suicide by inflicting multiple incised wounds on his neck four days before the safety blade ingestion, but none were fatal. © The Author(s) 2016.

Human HepaRG Cells can be Cultured in Hanging-drop Plates for Cytochrome P450 Induction and Function Assays.

PubMed

Murayama, Norie; Usui, Takashi; Slawny, Nicky; Chesné, Christophe; Yamazaki, Hiroshi

2015-01-01

Recent guidance/guidelines for industry recommend that cytochrome P450 induction can be assessed using human hepatocyte enzyme activity and/or mRNA levels to evaluate potential drug- drug interactions. To evaluate time-dependent cytochrome P450 induction precisely, induction of CYP1A2, CYP2B6, and CYP3A4 mRNA was confirmed (>2-fold) by the treatment with omeprazole, phenobarbital, and rifampicin, respectively, for 24 or 48 h on day 3 from the start of culture. After 24 h, the fold induction of CYP1A2 with 3.6 and 1.8x10(4) HepaRG cells per well was lower than that for 7.2x10(4) cells. CYP1A2 induction levels at 24 h were higher than those after 48 h. In contrast, higher CYP2B6 inductions were confirmed after 48 h exposure than after 24 h, independent of the number of cells per well. To help reduce the use of human cryopreserved hepatocytes, typical P450-dependent enzyme activities were investigated in human HepaRG cells cultured in commercial hanging-drop plates. Newly designed 96-well hanging-drop plates were capable of maintaining human CYP3A-dependent midazolam hydroxylation activities for up to 4 days using only 10% of the recommended initial 7.2x10(4) cells per well. Favorable HepaRG function using hanging-drop plates was confirmed by detecting 1'- hydroxymidazolam O-glucuronide on day 3, suggesting an improvement over traditional control plates in which this metabolite can be detected for 24-well plates. These results suggest that the catalytic function and/or induction of CYP1A2, CYP2B6, and CYP3A4 can be readily assessed with reduced numbers of starting HepaRG cells cultured in three-dimensional cultures in drops prepared with hanging-drop plates.

Lactococcus petauri sp. nov., isolated from an abscess of a sugar glider.

PubMed

Goodman, Laura B; Lawton, Marie R; Franklin-Guild, Rebecca J; Anderson, Renee R; Schaan, Lynn; Thachil, Anil J; Wiedmann, Martin; Miller, Claire B; Alcaine, Samuel D; Kovac, Jasna

2017-11-01

A strain of lactic acid bacteria, designated 159469 T , isolated from a facial abscess in a sugar glider, was characterized genetically and phenotypically. Cells of the strain were Gram-stain-positive, coccoid and catalase-negative. Morphological, physiological and phylogenetic data indicated that the isolate belongs to the genus Lactococcus. Strain 159469 T was closely related to Lactococcus garvieae ATCC 43921 T , showing 95.86 and 98.08 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively. Furthermore, a pairwise average nucleotide identity blast (ANIb) value of 93.54 % and in silico DNA-DNA hybridization value of 50.7  % were determined for the genome of strain 159469 T , when compared with the genome of the type strain of Lactococcus garvieae. Based on the data presented here, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus petauri sp. nov. is proposed. The type strain is 159469 T (=LMG 30040 T =DSM 104842 T ).

Optimal Estimation of Glider’s Underwater Trajectory with Depth-Dependent Correction Using the Navy Coastal Ocean Model with Application to Antisubmarine Warfare

DTIC Science & Technology

2014-09-01

deployed simultaneously. For example, a fleet of gliders would be able to act as an intelligence network by gathering underwater target information ...and to verify our novel method, a glider’s real underwater trajectory information must be obtained by using additional sensors like ADCP or DVL (see...lacks of inexpensive and efficient localization sensors during its subsurface mission. Therefore, knowing its precise underwater position is a

Complementary Use of Glider Data, Altimetry, and Model for Exploring Mesoscale Eddies in the Tropical Pacific Solomon Sea

NASA Astrophysics Data System (ADS)

Gourdeau, L.; Verron, J.; Chaigneau, A.; Cravatte, S.; Kessler, W.

2017-11-01

Mesoscale activity is an important component of the Solomon Sea circulation that interacts with the energetic low-latitude western boundary currents of the South Tropical Pacific Ocean carrying waters of subtropical origin before joining the equatorial Pacific. Mixing associated with mesoscale activity could explain water mass transformation observed in the Solomon Sea that likely impacts El Niño Southern Oscillation dynamics. This study makes synergetic use of glider data, altimetry, and high-resolution model for exploring mesoscale eddies, especially their vertical structures, and their role on the Solomon Sea circulation. The description of individual eddies observed by altimetry and gliders provides the first elements to characterize the 3-D structure of these tropical eddies, and confirms the usefulness of the model to access a more universal view of such eddies. Mesoscale eddies appear to have a vertical extension limited to the Surface Waters (SW) and the Upper Thermocline Water (UTW), i.e., the first 140-150 m depth. Most of the eddies are nonlinear, meaning that eddies can trap and transport water properties. But they weakly interact with the deep New Guinea Coastal Undercurrent that is a key piece of the equatorial circulation. Anticyclonic eddies are particularly efficient to advect salty and warm SW coming from the intrusion of equatorial Pacific waters at Solomon Strait, and to impact the characteristics of the New Guinea Coastal Current. Cyclonic eddies are particularly efficient to transport South Pacific Tropical Water (SPTW) anomalies from the North Vanuatu Jet and to erode by diapycnal mixing the high SPTW salinity.

Generation of multicellular tumor spheroids by the hanging-drop method.

PubMed

Timmins, Nicholas E; Nielsen, Lars K

2007-01-01

Owing to their in vivo-like characteristics, three-dimensional (3D) multicellular tumor spheroid (MCTS) cultures are gaining increasing popularity as an in vitro model of tumors. A straightforward and simple approach to the cultivation of these MCTS is the hanging-drop method. Cells are suspended in droplets of medium, where they develop into coherent 3D aggregates and are readily accessed for analysis. In addition to being simple, the method eliminates surface interactions with an underlying substratum (e.g., polystyrene plastic or agarose), requires only a low number of starting cells, and is highly reproducible. This method has also been applied to the co-cultivation of mixed cell populations, including the co-cultivation of endothelial cells and tumor cells as a model of early tumor angiogenesis.

Glider observations of the Dotson Ice Shelf outflow

NASA Astrophysics Data System (ADS)

Miles, Travis; Lee, Sang Hoon; Wåhlin, Anna; Ha, Ho Kyung; Kim, Tae Wan; Assmann, Karen M.; Schofield, Oscar

2016-01-01

The Amundsen Sea is one of the most productive polynyas in the Antarctic per unit area and is undergoing rapid changes including a reduction in sea ice duration, thinning ice sheets, retreat of glaciers and the potential collapse of the Thwaites Glacier in Pine Island Bay. A growing body of research has indicated that these changes are altering the water mass properties and associated biogeochemistry within the polynya. Unfortunately difficulties in accessing the remote location have greatly limited the amount of in situ data that has been collected. In this study data from a Teledyne-Webb Slocum glider was used to supplement ship-based sampling along the Dotson Ice Shelf (DIS). This autonomous underwater vehicle revealed a detailed view of a meltwater laden outflow from below the western flank of the DIS. Circumpolar Deep Water intruding onto the shelf drives glacial melt and the supply of macronutrients that, along with ample light, supports the large phytoplankton blooms in the Amundsen Sea Polynya. Less well understood is the source of micronutrients, such as iron, necessary to support this bloom to the central polynya where chlorophyll concentrations are highest. This outflow region showed decreasing optical backscatter with proximity to the bed indicating that particulate matter was sourced from the overlying glacier rather than resuspended sediment. This result suggests that particulate iron, and potentially phytoplankton primary productivity, is intrinsically linked to the magnitude and duration of sub-glacial melt from Circumpolar Deep Water intrusions onto the shelf.

Evaporation kinetics in the hanging drop method of protein crystal growth

NASA Technical Reports Server (NTRS)

Baird, James K.; Frieden, Richard W.; Meehan, E. J., Jr.; Twigg, Pamela J.; Howard, Sandra B.; Fowlis, William A.

1987-01-01

An engineering analysis of the rate of evaporation of solvent in the hanging drop method of protein crystal growth is presented; these results are applied to 18 different drop and well arrangements commonly encountered in the laboratory, taking into account the chemical nature of the salt, the drop size and shape, the drop concentration, the well size, the well concentration, and the temperature. It is found that the rate of evaporation increases with temperature, drop size, and with the salt concentration difference between the drop and the well. The evaporation possesses no unique half-life. Once the salt in the drop achieves about 80 percent of its final concentration, further evaporation suffers from the law of diminishing returns.

Rock around the Clock: Hanging Rock State Park. An Environmental Education Learning Experience. Designed for Grade 5.

ERIC Educational Resources Information Center

Dow, Jaye

This learning packet was developed for grade 5 to teach about the geology of a park. Sections include: (1) Introduction, which introduces the North Carolina State Park System, Hanging Rock State Park, the park's activity packet, and the geology of the park; (2) Summary, a brief summary of the activity outlines including major concepts and…

Mining and expression analysis of candidate genes involved in regulating the chilling requirement fulfillment of Paeonia lactiflora 'Hang Baishao'.

PubMed

Zhang, Jiaping; Li, Danqing; Shi, Xiaohua; Zhang, Dong; Qiu, Shuai; Wei, Jianfen; Zhang, Jiao; Zhou, Jianghua; Zhu, Kaiyuan; Xia, Yiping

2017-12-22

The artificial enlargement of the planting area and ecological amplitude of ornamentals for horticultural and landscape applications are significant. Herbaceous peony (Paeonia lactiflora Pall.) is a world-famous ornamental with attractive and fragrant flowers and is mainly planted in temperate and cool areas. Comparatively higher winter temperatures in the subtropical and tropical Northern Hemisphere result in a deficit of chilling accumulation for bud dormancy release, which severely hinders "The southward plantation of herbaceous peony". Studies on the dormancy, chilling requirement (CR) and relevant molecular mechanisms of peony are needed to enhance our ability to extend the range of this valuable horticultural species. Based on natural and artificial chilling experiments, and chilling hour (CH) and chilling unit (CU) evaluation systems, the lowest CR of 'Hang Baishao' was between 504.00 and 672.00 CHs and the optimal CR was 672.00 CHs and 856.08 CUs for achieving strong sprouting, growth and flowering performance. Transcriptome sequencing and gene identification by RNA-Seq were performed on 'Hang Baishao' buds during the dormancy and sprouting periods. Six gene libraries were constructed, and 66 temperature- and photoperiod-associated unigenes were identified as the potential candidate genes that may regulate or possibly determine CR characteristics. The difference in the expression patterns of SUPPRESSPOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) between the winters of 2012-2013 and 2015-2016, and the difference of CR fulfillment periods also between these two winters represented the interesting congruent relationships. This correlation was also observed for WRKY DNA-BINDING PROTEIN 33 (WRKY 33). Combined with the results acquired from all of experiments, 'Hang Baishao' was confirmed to be a superb peony resource that have significantly low CR characteristics. The two genes of SOC1 and WRKY33 are likely involved in determining the CR amount and fulfillment period

Simulation of a Single-Element Lean-Direct Injection Combustor Using a Polyhedral Mesh Derived from Hanging-Node Elements

NASA Technical Reports Server (NTRS)

Wey, Thomas; Liu, Nan-Suey

2013-01-01

This paper summarizes the procedures of generating a polyhedral mesh derived from hanging-node elements as well as presents sample results from its application to the numerical solution of a single element lean direct injection (LDI) combustor using an open-source version of the National Combustion Code (NCC).

Lactococcus petauri sp. nov., isolated from an abscess of a sugar glider

PubMed Central

Goodman, Laura B.; Lawton, Marie R.; Franklin-Guild, Rebecca J.; Anderson, Renee R.; Schaan, Lynn; Thachil, Anil J.; Wiedmann, Martin; Miller, Claire B.; Alcaine, Samuel D.; Kovac, Jasna

2017-01-01

A strain of lactic acid bacteria, designated 159469T, isolated from a facial abscess in a sugar glider, was characterized genetically and phenotypically. Cells of the strain were Gram-stain-positive, coccoid and catalase-negative. Morphological, physiological and phylogenetic data indicated that the isolate belongs to the genus Lactococcus. Strain 159469T was closely related to Lactococcus garvieae ATCC 43921T, showing 95.86 and 98.08 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively. Furthermore, a pairwise average nucleotide identity blast (ANIb) value of 93.54 % and in silico DNA–DNA hybridization value of 50.7  % were determined for the genome of strain 159469T, when compared with the genome of the type strain of Lactococcus garvieae. Based on the data presented here, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus petauri sp. nov. is proposed. The type strain is 159469T (=LMG 30040T=DSM 104842T). PMID:28945531

Langevin modelling of high-frequency Hang-Seng index data

NASA Astrophysics Data System (ADS)

Tang, Lei-Han

2003-06-01

Accurate statistical characterization of financial time series, such as compound stock indices, foreign currency exchange rates, etc., is fundamental to investment risk management, pricing of derivative products and financial decision making. Traditionally, such data were analyzed and modeled from a purely statistics point of view, with little concern on the specifics of financial markets. Increasingly, however, attention has been paid to the underlying economic forces and the collective behavior of investors. Here we summarize a novel approach to the statistical modeling of a major stock index (the Hang Seng index). Based on mathematical results previously derived in the fluid turbulence literature, we show that a Langevin equation with a variable noise amplitude correctly reproduces the ubiquitous fat tails in the probability distribution of intra-day price moves. The form of the Langevin equation suggests that, despite the extremely complex nature of financial concerns and investment strategies at the individual's level, there exist simple universal rules governing the high-frequency price move in a stock market.

Light and scanning electron microscopic study on the structure of the lingual papillae of the feathertail glider (Acrobates pygmeus, Burramyidae, Marsupialia).

PubMed

Jackowiak, Hanna; Godynicki, Szymon

2007-11-01

The structure of the tongue of the marsupial feathertail glider (Acrobates pygmeus) was observed under a light and scanning electron microscope. The elongated tongue with a sharpened apex is ca. 10 mm in length. Only the posterior half of the tongue is attached to the bottom of the oral cavity by the frenulum, which facilitates considerable mobility of the anterior free part of the tongue. On the dorsal surface of the tongue, three types of lingual papillae were distinguished, that is, mechanical filiform papillae and gustatory fungiform and vallate papillae. The arrangement, shape, and size of filiform papillae and the direction of their keratinized processes change depending on the part of the tongue, so that the surface of the apex and the body of the tongue resembles a brush adapted to effective holding of semiliquid food and collection of pollen. The fungiform papillae have a single taste bud and are uniformly scattered between filiform papillae only on the anterior half of the tongue. On the smooth root of the tongue, three oval vallate papillae are arranged in the form of a triangle, similarly as it is the case in other marsupials. The posterior biggest vallate papilla is oriented perpendicularly to the smaller anterior papillae. The results of the study on the feathertail glider show that the special arrangement of lingual papillae is strongly adapted to feeding behavior of this nectar-eating and frugivorous animal. Copyright 2007 Wiley-Liss, Inc.

Mean hydrography on the continental shelf from 26 repeat glider deployments along Southeastern Australia

NASA Astrophysics Data System (ADS)

Schaeffer, Amandine; Roughan, Moninya; Austin, Tim; Everett, Jason D.; Griffin, David; Hollings, Ben; King, Edward; Mantovanelli, Alessandra; Milburn, Stuart; Pasquer, Benedicte; Pattiaratchi, Charitha; Robertson, Robin; Stanley, Dennis; Suthers, Iain; White, Dana

2016-08-01

Since 2008, 26 glider missions have been undertaken along the continental shelf of southeastern Australia. Typically these missions have spanned the continental shelf on the inshore edge of the East Australian Current from 29.5-33.5°S. This comprehensive dataset of over 33,600 CTD profiles from the surface to within 10 m of the bottom in water depths ranging 25-200 m provides new and unprecedented high resolution observations of the properties of the continental shelf waters adjacent to a western boundary current, straddling the region where it separates from the coast. The region is both physically and biologically significant, and is also in a hotspot of ocean warming. We present gridded mean fields for temperature, salinity and density, but also dissolved oxygen and chlorophyll-a fluorescence indicative of phytoplankton biomass. This data will be invaluable for understanding shelf stratification, circulation, biophysical and bio-geochemical interactions, as well as for the validation of high-resolution ocean models or serving as teaching material.

Mean hydrography on the continental shelf from 26 repeat glider deployments along Southeastern Australia.

PubMed

Schaeffer, Amandine; Roughan, Moninya; Austin, Tim; Everett, Jason D; Griffin, David; Hollings, Ben; King, Edward; Mantovanelli, Alessandra; Milburn, Stuart; Pasquer, Benedicte; Pattiaratchi, Charitha; Robertson, Robin; Stanley, Dennis; Suthers, Iain; White, Dana

2016-08-30

Since 2008, 26 glider missions have been undertaken along the continental shelf of southeastern Australia. Typically these missions have spanned the continental shelf on the inshore edge of the East Australian Current from 29.5-33.5°S. This comprehensive dataset of over 33,600 CTD profiles from the surface to within 10 m of the bottom in water depths ranging 25-200 m provides new and unprecedented high resolution observations of the properties of the continental shelf waters adjacent to a western boundary current, straddling the region where it separates from the coast. The region is both physically and biologically significant, and is also in a hotspot of ocean warming. We present gridded mean fields for temperature, salinity and density, but also dissolved oxygen and chlorophyll-a fluorescence indicative of phytoplankton biomass. This data will be invaluable for understanding shelf stratification, circulation, biophysical and bio-geochemical interactions, as well as for the validation of high-resolution ocean models or serving as teaching material.

Muscle activity and spine load during anterior chain whole body linkage exercises: the body saw, hanging leg raise and walkout from a push-up.

PubMed

McGill, Stuart; Andersen, Jordan; Cannon, Jordan

2015-01-01

This study examined anterior chain whole body linkage exercises, namely the body saw, hanging leg raise and walkout from a push-up. Investigation of these exercises focused on which particular muscles were challenged and the magnitude of the resulting spine load. Fourteen males performed the exercises while muscle activity, external force and 3D body segment motion were recorded. A sophisticated and anatomically detailed 3D model used muscle activity and body segment kinematics to estimate muscle force, and thus sensitivity to each individual's choice of motor control for each task. Gradations of muscle activity and spine load characteristics were observed across tasks. On average, the hanging straight leg raise created approximately 3000 N of spine compression while the body saw created less than 2500 N. The hanging straight leg raise created the highest challenge to the abdominal wall (>130% MVC in rectus abdominis, 88% MVC in external oblique). The body saw resulted in almost 140% MVC activation of the serratus anterior. All other exercises produced substantial abdominal challenge, although the body saw did so in the most spine conserving way. These findings, along with consideration of an individual's injury history, training goals and current fitness level, should assist in exercise choice and programme design.

Turbulence and Mixing in a Shallow Shelf Sea From Underwater Gliders

NASA Astrophysics Data System (ADS)

Schultze, Larissa K. P.; Merckelbach, Lucas M.; Carpenter, Jeffrey R.

2017-11-01

The seasonal thermocline in shallow shelf seas acts as a natural barrier for boundary-generated turbulence, damping scalar transport to the upper regions of the water column and controlling primary production to a certain extent. To better understand turbulence and mixing conditions within the thermocline, two unique 12 and 17 day data sets with continuous measurements of the dissipation rate of turbulent kinetic energy (ɛ) collected by autonomous underwater gliders under stratified to well-mixed conditions are presented. A highly intermittent ɛ signal was observed in the stratified thermocline region, which was mainly characterized by quiescent flow (turbulent activity index below 7). The rate of diapycnal mixing remained relatively constant for the majority of the time with peaks of higher fluxes that were responsible for much of the increase in bottom mixed layer temperature. The water column stayed predominantly strongly stratified, with a bulk Richardson number across the thermocline well above 2. A positive relationship between the intensity of turbulence, shear, and stratification was found. The trend between turbulence levels and the bulk Richardson number was relatively weak but suggests that ɛ increases as the bulk Richardson number approaches 1. The results also highlight the interpretation difficulties in both quantifying turbulent thermocline fluxes as well as the responsible mechanisms.

Real-time Web GIS to monitor marine water quality using wave glider

NASA Astrophysics Data System (ADS)

Maneesa Amiruddin, Siti

2016-06-01

In the past decade, Malaysia has experienced unprecedented economic development and associated socioeconomic changes. As environmentalists anticipate these changes could have negative impacts on the marine and coastal environment, a comprehensive, continuous and long term marine water quality monitoring programme needs to be strengthened to reflect the government's aggressive mind-set of enhancing its authority in protection, preservation, management and enrichment of vast resources of the ocean. Wave Glider, an autonomous, unmanned marine vehicle provides continuous ocean monitoring at all times and is durable in any weather condition. Geographic Information System (GIS) technology is ideally suited as a tool for the presentation of data derived from continuous monitoring of locations, and used to support and deliver information to environmental managers and the public. Combined with GeoEvent Processor, an extension from ArcGIS for Server, it extends the Web GIS capabilities in providing real-time data from the monitoring activities. Therefore, there is a growing need of Web GIS for easy and fast dissemination, sharing, displaying and processing of spatial information which in turn helps in decision making for various natural resources based applications.

Automatic image hanging protocol for chest radiographs in PACS.

PubMed

Luo, Hui; Hao, Wei; Foos, David H; Cornelius, Craig W

2006-04-01

Chest radiography is one of the most widely used techniques in diagnostic imaging. It comprises at least one-third of all diagnostic radiographic procedures in hospitals. However, in the picture archive and communication system, images are often stored with the projection and orientation unknown or mislabeled, which causes inefficiency for radiologists' interpretation. To address this problem, an automatic hanging protocol for chest radiographs is presented. The method targets the most effective region in a chest radiograph, and extracts a set of size-, rotation-, and translation-invariant features from it. Then, a well-trained classifier is used to recognize the projection. The orientation of the radiograph is later identified by locating the neck, heart, and abdomen positions in the radiographs. Initial experiments are performed on the radiographs collected from daily routine chest exams in hospitals and show promising results. Using the presented protocol, 98.2% of all cases could be hung correctly on projection view (without protocol, 62%), and 96.1% had correct orientation (without protocol, 75%). A workflow study on the protocol also demonstrates a significant improvement in efficiency for image display.

Experimental and theoretical analysis of the rate of solvent equilibration in the hanging drop method of protein crystal growth

NASA Technical Reports Server (NTRS)

Fowlis, William W.; Delucas, Lawrence J.; Twigg, Pamela J.; Howard, Sandra B.; Meehan, Edward J.

1988-01-01

The principles of the hanging-drop method of crystal growth are discussed, and the rate of water evaporation in a water droplet (containing protein, buffer, and a precipitating agent) suspended above a well containing a double concentration of precipitating agent is investigated theoretically. It is shown that, on earth, the rate of evaporation may be determined from diffusion theory and the colligative properties of solutions. The parameters affecting the rate of evaporation include the temperature, the vapor pressure of water, the ionization constant of the salt, the volume of the drop, the contact angle between the droplet and the coverslip, the number of moles of salt in the droplet, the number of moles of water and salt in the well, the molar volumes of water and salt, the distance from the droplet to the well, and the coefficient of diffusion of water vapor through air. To test the theoretical equations, hanging-drop experiments were conducted using various reagent concentrations in 25-microliter droplets and measuring the evaporation times at 4 C and 25 C. The results showed good agreement with the theory.

Petrophysical, Geochemical, and Hydrological Evidence for Extensive Fracture-Mediated Fluid and Heat Transport in the Alpine Fault's Hanging-Wall Damage Zone

NASA Astrophysics Data System (ADS)

Townend, John; Sutherland, Rupert; Toy, Virginia G.; Doan, Mai-Linh; Célérier, Bernard; Massiot, Cécile; Coussens, Jamie; Jeppson, Tamara; Janku-Capova, Lucie; Remaud, Léa.; Upton, Phaedra; Schmitt, Douglas R.; Pezard, Philippe; Williams, Jack; Allen, Michael John; Baratin, Laura-May; Barth, Nicolas; Becroft, Leeza; Boese, Carolin M.; Boulton, Carolyn; Broderick, Neil; Carpenter, Brett; Chamberlain, Calum J.; Cooper, Alan; Coutts, Ashley; Cox, Simon C.; Craw, Lisa; Eccles, Jennifer D.; Faulkner, Dan; Grieve, Jason; Grochowski, Julia; Gulley, Anton; Hartog, Arthur; Henry, Gilles; Howarth, Jamie; Jacobs, Katrina; Kato, Naoki; Keys, Steven; Kirilova, Martina; Kometani, Yusuke; Langridge, Rob; Lin, Weiren; Little, Tim; Lukacs, Adrienn; Mallyon, Deirdre; Mariani, Elisabetta; Mathewson, Loren; Melosh, Ben; Menzies, Catriona; Moore, Jo; Morales, Luis; Mori, Hiroshi; Niemeijer, André; Nishikawa, Osamu; Nitsch, Olivier; Paris, Jehanne; Prior, David J.; Sauer, Katrina; Savage, Martha K.; Schleicher, Anja; Shigematsu, Norio; Taylor-Offord, Sam; Teagle, Damon; Tobin, Harold; Valdez, Robert; Weaver, Konrad; Wiersberg, Thomas; Zimmer, Martin

2017-12-01

Fault rock assemblages reflect interaction between deformation, stress, temperature, fluid, and chemical regimes on distinct spatial and temporal scales at various positions in the crust. Here we interpret measurements made in the hanging-wall of the Alpine Fault during the second stage of the Deep Fault Drilling Project (DFDP-2). We present observational evidence for extensive fracturing and high hanging-wall hydraulic conductivity (˜10-9 to 10-7 m/s, corresponding to permeability of ˜10-16 to 10-14 m2) extending several hundred meters from the fault's principal slip zone. Mud losses, gas chemistry anomalies, and petrophysical data indicate that a subset of fractures intersected by the borehole are capable of transmitting fluid volumes of several cubic meters on time scales of hours. DFDP-2 observations and other data suggest that this hydrogeologically active portion of the fault zone in the hanging-wall is several kilometers wide in the uppermost crust. This finding is consistent with numerical models of earthquake rupture and off-fault damage. We conclude that the mechanically and hydrogeologically active part of the Alpine Fault is a more dynamic and extensive feature than commonly described in models based on exhumed faults. We propose that the hydrogeologically active damage zone of the Alpine Fault and other large active faults in areas of high topographic relief can be subdivided into an inner zone in which damage is controlled principally by earthquake rupture processes and an outer zone in which damage reflects coseismic shaking, strain accumulation and release on interseismic timescales, and inherited fracturing related to exhumation.

Comparison of the Hang High-Pull and Loaded Jump Squat for the Development of Vertical Jump and Isometric Force-Time Characteristics.

PubMed

Oranchuk, Dustin J; Robinson, Tracey L; Switaj, Zachary J; Drinkwater, Eric J

2017-04-15

Weightlifting movements have high skill demands and require expert coaching. Loaded jumps have a comparably lower skill demand, but may be similarly effective for improving explosive performance. The purpose of this study was to compare vertical jump performance, isometric force, and rate of force development (RFD) following a ten-week intervention employing the hang high-pull (hang-pull) or trap-bar jump squat (jump-squat). Eighteen NCAA Division II swimmers (8 males, 10 females) with at least one year of resistance training experience volunteered to participate. Testing included the squat jump (SJ), countermovement jump (CMJ) and the isometric mid-thigh pull (IMTP). Vertical ground reaction forces were analyzed to obtain jump height and relative peak power. Relative peak force, peak RFD and relative force at five time bands were obtained from the IMTP. Subjects were randomly assigned to either a hang-pull (n = 9) or jump-squat (n = 9) training group and completed a ten-week, volume-equated, periodized training program. While there was a significant main effect of training for both groups, no statistically significant between-group differences were found (p ≥ 0.17) for any of the dependent variables. However, medium effect sizes in favor of the jump-squat training group were seen in SJ height (d = 0.56) and SJ peak power (d = 0.69). Loaded jumps seem equally effective as weightlifting derivatives for improving lower-body power in experienced athletes. Since loaded jumps require less skill and less coaching expertise than weightlifting, loaded jumps should be considered where coaching complex movements is difficult.

Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor.

PubMed

Konstantinou, Dimitrios; Lei, Ming; Xia, Zhidao; Kanamarlapudi, Venkateswarlu

2015-04-01

Heart disease is the major leading cause of death worldwide and the use of stem cells promises new ways for its treatment. The relatively easy and quick acquisition of human umbilical cord matrix mesenchymal stem cells (HUMSCs) and their properties make them useful for the treatment of cardiac diseases. Therefore, the main aim of this investigation was to create cardiac polymicrotissue from HUMSCs using a combination of growth factors [sphingosine-1-phosphate (S1P) and suramin] and techniques (hanging drop and bioreactor). Using designated culture conditions of the growth factors (100 nM S1P and 500 µM suramin), cardiomyocyte differentiation medium (CDM), hanging drop, bioreactor and differentiation for 7 days, a potential specific cardiac polymicrotissue was derived from HUMSCs. The effectiveness of growth factors alone or in combination in differentiation of HUMSCs to cardiac polymicrotissue was analysed by assessing the presence of cardiac markers by immunocytochemistry. This analysis demonstrated the importance of those growth factors for the differentiation. This study for the first time demonstrated the formation of a cardiac polymicrotissue under specific culture conditions. The polymicrotissue thus obtained may be used in future as a 'patch' to cover the injured cardiac region and would thereby be useful for the treatment of heart diseases. © 2014 International Federation for Cell Biology.

Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes.

PubMed

Shri, Meena; Agrawal, Himanshu; Rani, Payal; Singh, Dheer; Onteru, Suneel Kumar

2017-04-26

Livestock, having close resemblance to humans, could be a better source of primary hepatocytes than rodents. Herein, we successfully developed three-dimensional (3D) culturing system for primary sheep and buffalo hepatocytes. The 3D-structures of sheep hepatocytes were formed on the fifth-day and maintained until the tenth-day on polyHEMA-coated plates and in hanging drops with William's E media (HDW). Between the cultured and fresh cells, we observed a similar expression of GAPDH, HNF4α, ALB, CYP1A1, CK8 and CK18. Interestingly, a statistically significant increase was noted in the TAT, CPS, AFP, AAT, GSP and PCNA expression. In buffalo hepatocytes culture, 3D-like structures were formed on the third-day and maintained until the sixth-day on polyHEMA and HDW. The expression of HNF4α, GSP, CPS, AFP, AAT, PCNA and CK18 was similar between cultured and fresh cells. Further, a statistically significant increase in the TAT and CK8 expression, and a decrease in the GAPDH, CYP1A1 and ALB expression were noted. Among the culture systems, HDW maintained the liver transcript markers more or less similar to the fresh hepatocytes of the sheep and buffalo for ten and six days, respectively. Taken together, hanging drop is an efficient method for 3D culturing of primary sheep and buffalo hepatocytes.

Mouse ES cells have a potential to differentiate into odontoblast-like cells using hanging drop method.

PubMed

Kawai, R; Ozeki, N; Yamaguchi, H; Tanaka, T; Nakata, K; Mogi, M; Nakamura, H

2014-05-01

We examined whether mouse embryonic stem (ES) cells can differentiate into odontoblast-like cells without epithelial-mesenchymal interaction. Cells were cultured by the 'hanging drop' method using a collagen type-I scaffold (CS) combined with bone morphogenetic protein (BMP)-4 (CS/BMP-4). Expression of odontoblast-related mRNA and protein, and cell proliferation were performed by reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence staining and WST-1 assay, respectively. Cells potently expressed odontoblast-related cell marker mRNAs following induction of odontoblastic differentiation. Dentin sialophosphoprotein, a marker of mature odontoblasts, was strongly expressed in differentiated ES cells. The cells also acquired an odontoblast-like functional phenotype, as evidenced by the appearance of alkaline phosphatase activity and calcification. The cell-surface expression of α2, α6, αV and αVβ3 integrin proteins was rapidly upregulated in differentiated cells. Finally, anti-α2 integrin antibody suppressed the expression of odontoblastic markers in cells grown using this culture system, suggesting that α2 integrin expression in ES cells triggers their differentiation into odontoblast-like cells. Mouse ES cells cultured by the 'hanging drop' method are able to differentiate into cells with odontoblast-specific physiological functions and cell-surface integrin protein expression. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Imaging transport phenomena during lysozyme protein crystal growth by the hanging drop technique

NASA Astrophysics Data System (ADS)

Sethia Gupta, Anamika; Gupta, Rajive; Panigrahi, P. K.; Muralidhar, K.

2013-06-01

The present study reports the transport process that occurs during the growth of lysozyme protein crystals by the hanging drop technique. A rainbow schlieren technique has been employed for imaging changes in salt concentration. A one dimensional color filter is used to record the deflection of the light beam. An optical microscope and an X-ray crystallography unit are used to characterize the size, tetragonal shape and Bravais lattice constants of the grown crystals. A parametric study on the effect of drop composition, drop size, reservoir height and number of drops on the crystal size and quality is reported. Changes in refractive index are not large enough to create a meaningful schlieren image in the air gap between the drop and the reservoir. However, condensation of fresh water over the reservoir solution creates large changes in the concentration of NaCl, giving rise to clear color patterns in the schlieren images. These have been analyzed to obtain salt concentration profiles near the free surface of the reservoir solution as a function of time. The diffusion of fresh water into the reservoir solution at the early stages of crystal growth followed by the mass flux of salt from the bulk solution towards the free surface has been recorded. The overall crystal growth process can be classified into two regimes, as demarcated by the changes in slope of salt concentration within the reservoir. The salt concentration in the reservoir equilibrates at long times when the crystallization process is complete. Thus, transport processes in the reservoir emerge as the route to monitor protein crystal growth in the hanging drop configuration. Results show that crystal growth rate is faster for a higher lysozyme concentration, smaller drops, and larger reservoir heights.

Updates to Simulation of a Single-Element Lean-Direct Injection Combustor Using a Polyhedral Mesh Derived From Hanging-Node Elements

NASA Technical Reports Server (NTRS)

Wey, Changju Thomas; Liu, Nan-Suey

2014-01-01

This paper summarizes the procedures of inserting a thin-layer mesh to existing inviscid polyhedral mesh either with or without hanging-node elements as well as presents sample results from its applications to the numerical solution of a single-element LDI combustor using a releasable edition of the National Combustion Code (NCC).

Updates to Simulation of a Single-Element Lean-Direct Injection Combustor Using a Polyhedral Mesh Derived from Hanging-Node Elements

NASA Technical Reports Server (NTRS)

Wey, Thomas; Liu, Nan-Suey

2014-01-01

This paper summarizes the procedures of inserting a thin-layer mesh to existing inviscid polyhedral mesh either with or without hanging-node elements as well as presents sample results from its applications to the numerical solution of a single-element LDI combustor using a releasable edition of the National Combustion Code (NCC).

Study of the algorithm of backtracking decoupling and adaptive extended Kalman filter based on the quaternion expanded to the state variable for underwater glider navigation.

PubMed

Huang, Haoqian; Chen, Xiyuan; Zhou, Zhikai; Xu, Yuan; Lv, Caiping

2014-12-03

High accuracy attitude and position determination is very important for underwater gliders. The cross-coupling among three attitude angles (heading angle, pitch angle and roll angle) becomes more serious when pitch or roll motion occurs. This cross-coupling makes attitude angles inaccurate or even erroneous. Therefore, the high accuracy attitude and position determination becomes a difficult problem for a practical underwater glider. To solve this problem, this paper proposes backing decoupling and adaptive extended Kalman filter (EKF) based on the quaternion expanded to the state variable (BD-AEKF). The backtracking decoupling can eliminate effectively the cross-coupling among the three attitudes when pitch or roll motion occurs. After decoupling, the adaptive extended Kalman filter (AEKF) based on quaternion expanded to the state variable further smoothes the filtering output to improve the accuracy and stability of attitude and position determination. In order to evaluate the performance of the proposed BD-AEKF method, the pitch and roll motion are simulated and the proposed method performance is analyzed and compared with the traditional method. Simulation results demonstrate the proposed BD-AEKF performs better. Furthermore, for further verification, a new underwater navigation system is designed, and the three-axis non-magnetic turn table experiments and the vehicle experiments are done. The results show that the proposed BD-AEKF is effective in eliminating cross-coupling and reducing the errors compared with the conventional method.

Study of the Algorithm of Backtracking Decoupling and Adaptive Extended Kalman Filter Based on the Quaternion Expanded to the State Variable for Underwater Glider Navigation

PubMed Central

Huang, Haoqian; Chen, Xiyuan; Zhou, Zhikai; Xu, Yuan; Lv, Caiping

2014-01-01

High accuracy attitude and position determination is very important for underwater gliders. The cross-coupling among three attitude angles (heading angle, pitch angle and roll angle) becomes more serious when pitch or roll motion occurs. This cross-coupling makes attitude angles inaccurate or even erroneous. Therefore, the high accuracy attitude and position determination becomes a difficult problem for a practical underwater glider. To solve this problem, this paper proposes backing decoupling and adaptive extended Kalman filter (EKF) based on the quaternion expanded to the state variable (BD-AEKF). The backtracking decoupling can eliminate effectively the cross-coupling among the three attitudes when pitch or roll motion occurs. After decoupling, the adaptive extended Kalman filter (AEKF) based on quaternion expanded to the state variable further smoothes the filtering output to improve the accuracy and stability of attitude and position determination. In order to evaluate the performance of the proposed BD-AEKF method, the pitch and roll motion are simulated and the proposed method performance is analyzed and compared with the traditional method. Simulation results demonstrate the proposed BD-AEKF performs better. Furthermore, for further verification, a new underwater navigation system is designed, and the three-axis non-magnetic turn table experiments and the vehicle experiments are done. The results show that the proposed BD-AEKF is effective in eliminating cross-coupling and reducing the errors compared with the conventional method. PMID:25479331

Structural analysis of hanging wall and footwall blocks within the Río Guanajibo fold-and-thrust belt in Southwest Puerto Rico

NASA Astrophysics Data System (ADS)

Laó-Dávila, Daniel A.; Llerandi-Román, Pablo A.

2017-01-01

The Río Guanajibo fold-and-thrust belt (RGFT), composed of Cretaceous serpentinite and volcano-sedimentary rocks, represents the deformation front of a contractional event in SW Puerto Rico during the Paleogene. Previous studies inferred structural and stratigraphic relationships from poorly exposed outcrops. New road cuts exposed the Yauco (YF) and El Rayo Formations (ERF) providing insights on the deformation of the hanging wall and footwall. We described the nature and orientation of faults and folds and analyzed the kinematic indicators to characterize the deformation. The YF occurs in the hanging wall and shows a sequence of folded, medium-bedded mudstone and thinly bedded shale and sandstone. Major folds strike NW-SE and are gentle with steeply inclined axial planes and sub-horizontal fold axes. Minor folds are open with moderately inclined axial planes and gently to moderately inclined SE-plunging fold axes. NW-SE striking reverse and thrust faults cut layers and show movement to the SW. Steep left-lateral faults strike NW-SE and NE-SW, and smaller right-lateral strike-slip faults strike NNE-SSW. At the footwall, the ERF consists of bioclastic limestone and polymictic orthoconglomerates and paraconglomerates. Reverse and strike-slip faults cut along lithological contacts. Results suggest that the hanging wall and footwall accommodated strain along preexisting weaknesses, which are dependent on lithology and sedimentary structures. The kinematic analysis suggests that shortening in the NE-SW direction was partitioned between folding and interlayer shortening, accommodated by flexural slip, and reverse and left-lateral faults that resulted from contraction. The RGFT represents the Paleogene back arc deformation of a bivergent thrust system.

Controls on fault zone structure and brittle fracturing in the foliated hanging wall of the Alpine Fault

NASA Astrophysics Data System (ADS)

Williams, Jack N.; Toy, Virginia G.; Massiot, Cécile; McNamara, David D.; Smith, Steven A. F.; Mills, Steven

2018-04-01

Three datasets are used to quantify fracture density, orientation, and fill in the foliated hanging wall of the Alpine Fault: (1) X-ray computed tomography (CT) images of drill core collected within 25 m of its principal slip zones (PSZs) during the first phase of the Deep Fault Drilling Project that were reoriented with respect to borehole televiewer images, (2) field measurements from creek sections up to 500 m from the PSZs, and (3) CT images of oriented drill core collected during the Amethyst Hydro Project at distances of ˜ 0.7-2 km from the PSZs. Results show that within 160 m of the PSZs in foliated cataclasites and ultramylonites, gouge-filled fractures exhibit a wide range of orientations. At these distances, fractures are interpreted to have formed at relatively high confining pressures and/or in rocks that had a weak mechanical anisotropy. Conversely, at distances greater than 160 m from the PSZs, fractures are typically open and subparallel to the mylonitic or schistose foliation, implying that fracturing occurred at low confining pressures and/or in rocks that were mechanically anisotropic. Fracture density is similar across the ˜ 500 m width of the field transects. By combining our datasets with measurements of permeability and seismic velocity around the Alpine Fault, we further develop the hierarchical model for hanging-wall damage structure that was proposed by Townend et al. (2017). The wider zone of foliation-parallel fractures represents an outer damage zone that forms at shallow depths. The distinct < 160 m wide interval of widely oriented gouge-filled fractures constitutes an inner damage zone. This zone is interpreted to extend towards the base of the seismogenic crust given that its width is comparable to (1) the Alpine Fault low-velocity zone detected by fault zone guided waves and (2) damage zones reported from other exhumed large-displacement faults. In summary, a narrow zone of fracturing at the base of the Alpine Fault

Determination of nitrobenzene in wastewater using a hanging mercury drop electrode.

PubMed

Liang, Shu-Xuan; Zhang, Huan-Kun; Lu, Da

2007-06-01

The determination of trace amount nitrobenzene in wastewater on a hanging mercury drop electrode was studied. The determination conditions of pH, supporting electrolyte, accumulation potential, accumulation time, and voltammetric response were optimized. The sharp peak of the nitrobenzene was appeared at 0.05 V. The peak electric current was proportional to the concentration of nitrobenzene in the range of 1.47 x 10(-5) approximately 1.0 x 10(-3) mol/l with relative standard deviations of 3.99 approximately 8.94%. The detection limit of the nitrobenzene in water was 5 x 10(-6) mol/l. The proposed method offered low limit of determination, easy operation, the use of simple instrumentation, high sensitivity and good reproducibility. It was applied to the determination of nitrobenzene in wastewater with an average recovery of 94.0% approximately 105%. The proposed method provided fast, sensitive and sometimes real time detection of nitrobenzene.

Mean hydrography on the continental shelf from 26 repeat glider deployments along Southeastern Australia

PubMed Central

Schaeffer, Amandine; Roughan, Moninya; Austin, Tim; Everett, Jason D.; Griffin, David; Hollings, Ben; King, Edward; Mantovanelli, Alessandra; Milburn, Stuart; Pasquer, Benedicte; Pattiaratchi, Charitha; Robertson, Robin; Stanley, Dennis; Suthers, Iain; White, Dana

2016-01-01

Since 2008, 26 glider missions have been undertaken along the continental shelf of southeastern Australia. Typically these missions have spanned the continental shelf on the inshore edge of the East Australian Current from 29.5–33.5°S. This comprehensive dataset of over 33,600 CTD profiles from the surface to within 10 m of the bottom in water depths ranging 25–200 m provides new and unprecedented high resolution observations of the properties of the continental shelf waters adjacent to a western boundary current, straddling the region where it separates from the coast. The region is both physically and biologically significant, and is also in a hotspot of ocean warming. We present gridded mean fields for temperature, salinity and density, but also dissolved oxygen and chlorophyll-a fluorescence indicative of phytoplankton biomass. This data will be invaluable for understanding shelf stratification, circulation, biophysical and bio-geochemical interactions, as well as for the validation of high-resolution ocean models or serving as teaching material. PMID:27575831

New insights of the Northern Current in the Western Mediterranean Sea from Gliders data: Mean structure, Transport, and Seasonal Variability

NASA Astrophysics Data System (ADS)

Bosse, Anthony; Testor, Pierre; Mortier, Laurent; Beguery, Laurent; Bernardet, Karim; Taillandier, Vincent; d'Ortenzio, Fabrizio; Prieur, Louis; Coppola, Laurent; Bourrin, François

2013-04-01

In the last 5 years, an unprecedented effort in the sampling of the Northern Current (NC) has been carried out using gliders which collected more than 50 000 profiles down to 1000m maximum along a few repeated sections perpendicular to the French coast. Based on this dataset, this study presents a very first quantitative picture of the NC on 0-1000m depth. We show its mean structure of temperature and salinity characterized by the different Water Masses of the basin (Atlantic Water, Winter Intermediate Water, Levantine Intermediate Water and Western Mediterranean Deep Water) for each season and at different location. Geostrophic currents are derived from the integration of the thermal-wind balance using the mean glider-estimate of the current during each dive as a reference. Estimates of the heat, salt, and volume transport are then computed in order to draw an heat and salt budget of the NC. The results show a strong seasonal variability due to the intense surface buoyancy loss in winter resulting in a vertical mixing offshore that makes the mixed layer depth reaching several hundreds of meters in the whole basin and in a very particular area down to the bottom of the sea-floor (deep convection area). The horizontal density gradient intensifies in winter leading to geostrophic currents that are more intense and more confined to the continental slope, and thus to the enhancement of the mesoscale activity (meandering, formation of eddies through baroclinic instability...). The mean transport estimates of the NC is found to be about 2-3Sv greater than previous spurious estimates. The heat budget of the NC also provides an estimate of the mean across shore heat/salt flux directly impacting the region in the Gulf of Lion where deep ocean convection, a key process in the thermohaline circulation of the Mediterranean Sea, can occur in Winter.

Instructor perceptions of the accident likelihood faced by recently trained glider pilots.

PubMed

Jarvis, Steve; Harris, Don

2011-12-01

U.K. glider pilots with less than 10 h of solo flying time have been shown to have the highest accident rate and be most vulnerable to accidents during the 'final approach' phase. There were 58 gliding instructors who were asked to indicate what experience level they thought was associated with the highest accident rate and provide the reason behind their estimate. They were also asked to rank six flight phases by the relative probability of accidents to inexperienced pilots. The mean estimate for the accident peak was 296.3 h as pilot-in-command (SD = 337.9) with no instructor giving a figure of less than 10 h. Common reasons for these estimates were 'over-confidence', 'risk-taking', or 'complacency'. Instructors also ranked six flight phases by the likelihood of an accident being caused by inexperienced pilots during that phase. Despite the approach phase having the highest objective accident probability, it was only ranked fifth by instructors, indicating an underestimate of the danger it presents to newly trained pilots. The results suggest that instructors do not appreciate the high accident likelihood of early solo pilots or the main dangers they face. This has implications for the decisions made when sending pilots solo.

Hanging drop culture enhances differentiation of human adipose-derived stem cells into anterior neuroectodermal cells using small molecules.

PubMed

Amirpour, Noushin; Razavi, Shahnaz; Esfandiari, Ebrahim; Hashemibeni, Batoul; Kazemi, Mohammad; Salehi, Hossein

2017-06-01

Inspired by in vivo developmental process, several studies were conducted to design a protocol for differentiating of mesenchymal stem cells into neural cells in vitro. Human adipose-derived stem cells (hADSCs) as mesenchymal stem cells are a promising source for this purpose. At current study, we applied a defined neural induction medium by using small molecules for direct differentiation of hADSCs into anterior neuroectodermal cells. Anterior neuroectodermal differentiation of hADSCs was performed by hanging drop and monolayer protocols. At these methods, three small molecules were used to suppress the BMP, Nodal, and Wnt signaling pathways in order to obtain anterior neuroectodermal (eye field) cells from hADSCs. After two and three weeks of induction, the differentiated cells with neural morphology expressed anterior neuroectodermal markers such as OTX2, SIX3, β-TUB III and PAX6. The protein expression of such markers was confirmed by real time, RT-PCR and immunocytochemistry methods According to our data, it seems that the hanging drop method is a proper approach for neuroectodermal induction of hADSCs. Considering wide availability and immunosuppressive properties of hADSCs, these cells may open a way for autologous cell therapy of neurodegenerative disorders. Copyright © 2017 ISDN. Published by Elsevier Ltd. All rights reserved.

Seamless Combination of Fluorescence-Activated Cell Sorting and Hanging-Drop Networks for Individual Handling and Culturing of Stem Cells and Microtissue Spheroids.

PubMed

Birchler, Axel; Berger, Mischa; Jäggin, Verena; Lopes, Telma; Etzrodt, Martin; Misun, Patrick Mark; Pena-Francesch, Maria; Schroeder, Timm; Hierlemann, Andreas; Frey, Olivier

2016-01-19

Open microfluidic cell culturing devices offer new possibilities to simplify loading, culturing, and harvesting of individual cells or microtissues due to the fact that liquids and cells/microtissues are directly accessible. We present a complete workflow for microfluidic handling and culturing of individual cells and microtissue spheroids, which is based on the hanging-drop network concept: The open microfluidic devices are seamlessly combined with fluorescence-activated cell sorting (FACS), so that individual cells, including stem cells, can be directly sorted into specified culturing compartments in a fully automated way and at high accuracy. Moreover, already assembled microtissue spheroids can be loaded into the microfluidic structures by using a conventional pipet. Cell and microtissue culturing is then performed in hanging drops under controlled perfusion. On-chip drop size control measures were applied to stabilize the system. Cells and microtissue spheroids can be retrieved from the chip by using a parallelized transfer method. The presented methodology holds great promise for combinatorial screening of stem-cell and multicellular-spheroid cultures.

A Hanged From the Past: Medical Consideration on the Judas Iscariot Fresco-Chapelle Notre-Dame-des-Fontaines, La Brigue (15th Century).

PubMed

Gaeta, Raffaele; Fornaciari, Antonio

2016-09-01

The medieval chapel of Notre Dame-des-Fontaines (Our Lady of the Fountains), in the French Maritime Alps, is entirely covered by the fresco cycle of the Passion (15th century) that depicts the last days of Jesus from the Last Supper to the Resurrection. Under a small window, there is the brutal representation of the suicide of Judas Iscariot, hanging from a tree, with the abdomen quartered from which his soul, represented by a small man, is kidnapped by a devil. The author, Giovanni Canavesio, represented the traitor's death with very detailed anatomical structures, differently thus from other paintings of the same subject; it is therefore possible to assume that the artist had become familiar with the human anatomy. In particular, the realism of the hanged man's posture, neck bent in an unnatural way, allows us to hypothesize that it probably comes from direct observation of the executions of capital punishment, not infrequently imposed by the public authorities in low medieval Italy.

The oceanic variability of the Lofoten basin: first results from the glider activity of the ProVoLo project

NASA Astrophysics Data System (ADS)

Bosse, Anthony; Fer, Ilker

2017-04-01

Located in the northern Norwegian Sea at high latitude between 68°N and 73°N, the Lofoten basin is one of the world's most energetic areas regarding the ocean dynamics. It hosts the largest and deepest pool of warm Atlantic Waters in the Nordic Seas, thus leading to very intense air-sea energy fluxes and deep convection in winter. Understanding the physical processes involved in the water mass transformations of this very productive area is thus of crucial interest in a climate perspective, as well as for the fishery economics. The ProVoLo project aims at quantifying the energy pathways from the large-scale circulation to the (sub-)mesoscale, and eventually to the dissipation scale. To this end, the project is largely devoted to in situ observations involving R/V cruises (CTD, LADCP, microstructure), mooring lines, gliders (CTD and microstructure) and RAFOS floats. Collecting data with gliders in such a dynamical environment is a challenge. We present results from two completed Seaglider missions of 8-months duration each, started in May 2016, as well as from three ongoing missions. The observations enable the description of two key features of the Lofoten basin circulation: 1 - The Lofoten Basin eddy, which is permanent anticyclonic vortex that has been regularly detected in the center of the basin over the last decades. The vortex has very intense subsurface peak velocities exceeding 0.7 m/s and a small radius of about 15 km. The collected data also enable a description of the seasonal variability associated with the vortex, and give insight into its interaction with higher frequency flows. 2 - The frontal region situated along the Mohn ridge. The front is characterized by a narrow ( 15 km) and intense baroclinic jet separating the warm Atlantic waters from the cold waters coming from the Arctic. The observations from intensive sampling of this front, testify an important variability, at both seasonal time scale and from meso to submesoscale.

The Intelligent Control System and Experiments for an Unmanned Wave Glider.

PubMed

Liao, Yulei; Wang, Leifeng; Li, Yiming; Li, Ye; Jiang, Quanquan

2016-01-01

The control system designing of Unmanned Wave Glider (UWG) is challenging since the control system is weak maneuvering, large time-lag and large disturbance, which is difficult to establish accurate mathematical model. Meanwhile, to complete marine environment monitoring in long time scale and large spatial scale autonomously, UWG asks high requirements of intelligence and reliability. This paper focuses on the "Ocean Rambler" UWG. First, the intelligent control system architecture is designed based on the cerebrum basic function combination zone theory and hierarchic control method. The hardware and software designing of the embedded motion control system are mainly discussed. A motion control system based on rational behavior model of four layers is proposed. Then, combining with the line-of sight method(LOS), a self-adapting PID guidance law is proposed to compensate the steady state error in path following of UWG caused by marine environment disturbance especially current. Based on S-surface control method, an improved S-surface heading controller is proposed to solve the heading control problem of the weak maneuvering carrier under large disturbance. Finally, the simulation experiments were carried out and the UWG completed autonomous path following and marine environment monitoring in sea trials. The simulation experiments and sea trial results prove that the proposed intelligent control system, guidance law, controller have favorable control performance, and the feasibility and reliability of the designed intelligent control system of UWG are verified.

The Intelligent Control System and Experiments for an Unmanned Wave Glider

PubMed Central

Liao, Yulei; Wang, Leifeng; Li, Yiming; Li, Ye; Jiang, Quanquan

2016-01-01

The control system designing of Unmanned Wave Glider (UWG) is challenging since the control system is weak maneuvering, large time-lag and large disturbance, which is difficult to establish accurate mathematical model. Meanwhile, to complete marine environment monitoring in long time scale and large spatial scale autonomously, UWG asks high requirements of intelligence and reliability. This paper focuses on the “Ocean Rambler” UWG. First, the intelligent control system architecture is designed based on the cerebrum basic function combination zone theory and hierarchic control method. The hardware and software designing of the embedded motion control system are mainly discussed. A motion control system based on rational behavior model of four layers is proposed. Then, combining with the line-of sight method(LOS), a self-adapting PID guidance law is proposed to compensate the steady state error in path following of UWG caused by marine environment disturbance especially current. Based on S-surface control method, an improved S-surface heading controller is proposed to solve the heading control problem of the weak maneuvering carrier under large disturbance. Finally, the simulation experiments were carried out and the UWG completed autonomous path following and marine environment monitoring in sea trials. The simulation experiments and sea trial results prove that the proposed intelligent control system, guidance law, controller have favorable control performance, and the feasibility and reliability of the designed intelligent control system of UWG are verified. PMID:28005956

Increased sensitivity of anodic stripping voltammetry at the hanging mercury drop electrode by ultracathodic deposition.

PubMed

Rodrigues, José A; Rodrigues, Carlos M; Almeida, Paulo J; Valente, Inês M; Gonçalves, Luís M; Compton, Richard G; Barros, Aquiles A

2011-09-09

An improved approach to the anodic stripping voltammetric (ASV) determination of heavy metals, using the hanging mercury drop electrode (HMDE), is reported. It was discovered that using very cathodic accumulation potentials, at which the solvent reduction occurs (overpotential deposition), the voltammetric signals of zinc(II), cadmium(II), lead(II) and copper(II) increase. When compared with the classical methodology a 5 to 10-fold signal increase is obtained. This effect is likely due to both mercury drop oscillation at such cathodic potentials and added local convection at the mercury drop surface caused by the evolution of hydrogen bubbles. Copyright © 2011 Elsevier B.V. All rights reserved.

Model for determining vapor equilibrium rates in the hanging drop method for protein crystal growth

NASA Technical Reports Server (NTRS)

Baird, James K.; Frieden, Richard W.; Meehan, E. J., Jr.; Twigg, Pamela J.; Howard, Sandra B.; Fowlis, William A.

1987-01-01

An engineering analysis of the rate of evaporation of solvent in the hanging drop method of protein crystal growth is presented. Results are applied to 18 drop and well arrangements commonly encountered in the laboratory. The chemical nature of the salt, drop size and shape, drop concentration, well size, well concentration, and temperature are taken into account. The rate of evaporation increases with temperature, drop size, and the salt concentration difference between the drop and the well. The evaporation in this model possesses no unique half-life. Once the salt in the drop achieves 80 percent of its final concentration, further evaporation suffers from the law of diminishing returns.

Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method.

PubMed

Chen, Ming; Lin, Yong-Qing; Xie, Shuang-Lun; Wu, Hong-Fu; Wang, Jing-Feng

2011-04-01

Hanging drop (HD) culture is used to induce differentiation of embryonic stem cells (ESCs) into other cell types including cardiomyocytes. However, the factors affecting cardiac differentiation of ESCs with this method remain incompletely understood. We have investigated the effects of the starting number of ESCs in embryoid bodies (EBs) and the time of EB adherence to gelatin-coated plates on cardiac differentiation: cardiac differentiation was increased in the EBs by a larger number of ESCs and was decreased by plating EBs at day 4 or earlier. These two factors can thus be optimized to enrich the cardiac differentiation in ESCs using the HD method.

DFS Dive-control Brakes for Gliders and Airplanes ; And, Analytical Study of the Drag of the DFS Dive-control Brake

NASA Technical Reports Server (NTRS)

Jacobs, Hans; Wanner, Adolf

1940-01-01

These two reports are surveys on the progress and present state of development of dive-control flaps for gliders and airplanes. The second article describes how on the basis of wind tunnel and free-flight tests, the drag increase on brake flaps of the type DFS, can be predicted. Pressure records confirm a two-dimensional load distribution along the brake-flap surface Aerodynamically, the location of the brake flaps along the span is of importance for reasons of avoidance of vibration and oscillation phenomena on control and tail surfaces; statically, because of the magnitude of the frontal drag in diving with respect to the bending moments, which may become decisive for the dimensions of the wing attachment and for the wing covering.

Optimal formation of genetically modified and functional pancreatic islet spheroids by using hanging-drop strategy.

PubMed

Kim, H J; Alam, Z; Hwang, J W; Hwang, Y H; Kim, M J; Yoon, S; Byun, Y; Lee, D Y

2013-03-01

Rejection and hypoxia are important factors causing islet loss at an early stage after pancreatic islet transplantation. Recently, islets have been dissociated into single cells for reaggregation into so-called islet spheroids. Herein, we used a hanging-drop strategy to form islet spheroids to achieve functional equivalence to intact islets. To obtain single islet cells, we dissociated islets with trypsin-EDTA digestion for 10 minutes. To obtain spheroids, we dropped various numbers of single cells (125, 250, or 500 cells/30 μL drop) onto a Petri dish, that was inverted for incubation in humidified air containing 5% CO(2) at 37 °C for 7 days. The aggregated spheroids in the droplets were harvested for further culture. The size of the aggregated islet spheroids depended on the number of single cells (125-500 cells/30 μL droplet). Their morphology was similar to that of intact islets without any cellular damage. When treated with various concentrations of glucose to evaluate responsiveness, their glucose-mediated stimulation index value was similar to that of intact islets, an observation that was attributed to strong cell-to-cell interactions in islet spheroids. However, islet spheroids aggregated in general culture dishes showed abnormal glucose responsiveness owing to weak cell-to-cell interactions. Cell-to-cell interactions in islet spheroids were confirmed with an anti-connexin-36 monoclonal antibody. Finally, nonviral poly(ethylene imine)-mediated interleukin-10 cytokine gene delivered beforehand into dissociated single cells before formation of islet spheroids increased the gene transfection efficacy and interleukin-10 secretion from islet spheroids >4-fold compared with intact islets. These results demonstrated the potential application of genetically modified, functional islet spheroids with of controlled size and morphology using an hanging-drop technique. Copyright © 2013 Elsevier Inc. All rights reserved.

High sub-seasonal variability in water volume transports, revealed through a new ocean monitoring initiative using autonomous gliders

NASA Astrophysics Data System (ADS)

Heslop, E.; Ruiz, S.; Allen, J.; Tintoré, J.

2012-04-01

One of the clear challenges facing oceanography today is to define variability in ocean processes at a seasonal and sub-seasonal scale, in order to clearly identify the signature of both natural large-scale climatic oscillations and the long-term trends brought about by the human-induced change in atmospheric composition. Without visibility of this variance, which helps to determine the margins of significance for long-term trends and decipher cause and effect, the inferences drawn from sparse data points can be misleading. The cyclonic basin scale circulation pattern in the Western Mediterranean has long been known; the role/contribution that processes in the Balearic Basin play in modifying this is less well defined. The Balearic Channels (channels between the Balearic Islands) are constriction points on this basin scale circulation that appear to exert a controlling influence on the north/south exchange of water masses. Understanding the variability in current flows through these channels is important, not just for the transport of heat and salt, but also for ocean biology that responds to physical variability at the scale of that variability. Earlier studies at a seasonal scale identified; an interannual summer/winter variation of 1 Sv in the strength of the main circulation pattern and a high cruise-to-cruise variability in the pattern and strength of the flows through the channels brought about by mesoscale activity. Initial results using new high-resolution data from glider based monitoring missions across the Ibiza Channel (the main exchange channel in the Balearic Basin), combined with ship and contemporaneous satellite data, indicate surprisingly high and rapid changes in the flows of surface and intermediate waters imposed on the broad seasonal cycle. To date the data suggests that there are three potential 'modes' of water volume transport, generated from the interplay between basin and mesoscale circulation. We will review the concept of transport

Voltammetric analysis of N-containing drugs using the hanging galinstan drop electrode (HGDE).

PubMed

Channaa, H; Surmann, P

2009-03-01

The electrochemical behaviour of several N-containing voltammetric active drugs such as 1,4-benzodiazepines (chlordiazepoxide, nitrazepam and diazepam) as well as one nitro-compound (nitrofurantoin) and one azo-compound (phenazopyridine) is described using a new kind of liquid electrode, the hanging galinstan drop electrode. Concentrations of 10(-5) - 10(-8) mol L(-1) are generally measurable. Differential pulse and adsorptive stripping voltammograms are recorded in different supporting electrolytes, like 0.1 M KNO3, acetate buffer solution pH = 4.6 and phosphate buffer solution pH = 7.0. The effects of varying the starting potentials, U(start) for DPV and accumulation times, t(acc) for AdSV are considered. Briefly, it is shown that the novel galinstan electrode is suitable for reducing several functional groups in organic substances, here presented for N-oxide-, azomethine-, nitro- and azo-groups.

Validation of HF Radar ocean surface currents in the Ibiza Channel using lagrangian drifters, moored current meter and underwater gliders

NASA Astrophysics Data System (ADS)

Lana, Arancha; Fernández, Vicente; Orfila, Alejandro; Troupin, Charles; Tintoré, Joaquín

2015-04-01

SOCIB High Frequency (HF) radar is one component of a multi-platform system located in the Balearic Islands and made up of Lagrangian platforms (profilers and drifting buoys), fixed stations (sea-level, weather, mooring and coastal), beach monitoring (camera), gliders, a research vessel as well as an ocean forecast system (waves and hydrodynamics). The HF radar system overlooks the Ibiza Channel, known as a 'choke point" where Atlantic and Mediterranean water masses interact and where meridional exchanges of water mass properties between the Balearic and the Algerian sub-basins take place. In order to determine the reliability of surface velocity measurements in this area, a quality assessment of the HF Radar is essential. We present the results of several validation experiments performed in the Ibiza Channel in 2013 and 2014. Of particular interest is an experiment started in September 2014 when a set of 13 surface drifters with different shapes and drogue lengths were released in the area covered by the HF radar. The drifter trajectories can be examined following the SOCIB Deployment Application (DAPP): http://apps.socib.es/dapp. Additionally, a 1-year long time series of surface currents obtained from a moored surface current-meter located in the Ibiza Channel, inside the area covered by the HF radar, was also used as a useful complementary validation exercise. Direct comparison between both radial surface currents from each radar station and total derived velocities against drifters and moored current meter velocities provides an assessment of the HF radar data quality at different temporal periods and geographical areas. Statistics from these comparisons give good correlation and low root-mean-square deviation. The results will be discussed for different months, geographical areas and types of surface drifters and wind exposure. Moreover, autonomous underwater glider constitutes an additional source of information for the validation of the observed velocity

Searching for new features of intravitality of hanging based on macro- and microscopic evaluation of the proximal attachment of the sternocleidomastoid muscle and the mastoid process of the temporal bone.

PubMed

Szleszkowski, Ł; Hałoń, A; Thannhäuser, A; Jurek, T

2015-01-01

Assessment of the usefulness of intravital lesions in the proximal attachment of the sternocleidomastoid muscle and the mastoid process of the temporal bone in medico-legal evaluation of death by hanging. The study material was obtained from the bodies of 35 people who died by hanging. The control group comprised specimens collected from 30 people who died of non-traumatic causes. The structures under study were examined macro- and microscopically. The basic change which could be recognized as a marker of intravitality of hanging was the presence of a macroscopically extensive blotchy area of abundant ecchymosis in the proximal muscle attachment, similar to that found in the distal attachment, and the presence of abundant diffuse intraosseous ecchymoses in the mastoid process. None of the cases revealed any ecchymoses in the proximal attachment of the muscle that would be similar to those present in the distal attachment. Discolourations within the mastoid processes, macroscopically suggestive of extensive intraosseous effusions arising from the mechanism of stretching, were not confirmed by microscopic evaluation and occurred at the same frequency as in the control group. Limitations of the study were related to the method which involved sample collection by means of bone chisels, decalcification and preparation of specimens, which had an effect, for example, on the measurable evaluation of the degree of congestion. The study has failed to provide convincing and unambiguous data on the usefulness of examining mastoid processes and proximal attachments of the sternocleidomastoid muscles during autopsy to determine the presence of intravitality features of hanging. A description of research methodology and its associated difficulties, e.g. with the interpretation of results, can also be useful for the planning of similar studies by other researchers.

The effect of body position on the 'hanging drop' method for identifying the extradural space in anaesthetized dogs.

PubMed

Naganobu, Kiyokazu; Hagio, Mitsuyoshi

2007-01-01

To assess the accuracy of the 'hanging drop method' for identifying the extradural space in anaesthetized dogs positioned in sternal or lateral recumbency. Prospective randomized-experimental study. Seventeen clinically healthy adult dogs, 10 females and seven males weighing 8.4-26.2 kg. Dogs were positioned in either sternal (n = 8) or lateral (n = 9) recumbency under general anaesthesia. A 20 SWG spinal needle pre-filled with 0.9% saline was advanced through the skin into the lumbosacral extradural space and the response of the saline drop recorded, i.e. whether it: 1) was aspirated from the hub into the needle; 2) remained within the hub, or 3) moved synchronously with i) spontaneous respiration, ii) heart beat or iii) manual lung inflation. The position of the needle tip was ultimately determined by positive contrast radiography. One dog positioned in lateral recumbency was excluded from the study because bleeding occurred from the needle hub. Saline was aspirated into the needle in seven of eight dogs held in sternal recumbency but in none of the dogs positioned in lateral recumbency. Accurate needle tip placement in the extradural space was confirmed by positive contrast radiography in all dogs. The 'hanging drop' method, when performed with a spinal needle, appears to be a useful technique for identifying the location of the extradural space in anaesthetized medium-sized dogs positioned in sternal, but not in lateral recumbency. The technique may yield 'false negative' results when performed in dogs positioned in sternal recumbency.

Influence of Knee-to-Feet Jump Training on Vertical Jump and Hang Clean Performance.

PubMed

Stark, Laura; Pickett, Karla; Bird, Michael; King, Adam C

2016-11-01

Stark, L, Pickett, K, Bird, M, and King, AC. Influence of knee-to-feet jump training on vertical jump and hang clean performance. J Strength Cond Res 30(11): 3084-3089, 2016-From a motor learning perspective, the practice/training environment can result in positive, negative, or neutral transfer to the testing conditions. The purpose of this study was to examine the training effect of a novel movement (knee-to-feet [K2F] jumps) and whether a 6-week training program induced a positive transfer effect to other power-related movements (vertical jump and hang clean [HC]). Twenty-six intercollegiate athletes from power-emphasized sports were paired and counter-balanced into a control (i.e., maintained their respective sport-specific lifting regimen) or an experimental group (i.e., completed a 6-week progressive training program of K2F jumps in addition to respective lifting regimen). A pre- and posttest design was used to investigate the effect of training on K2F jump height and transfer effect to vertical jump height (VJH) and 2-repetition maximum (RM) HC performance. A significant increase in K2F jump height was found for the experimental group. Vertical jump height significantly increased from pre- to posttest but no group or interaction (group × time) effect was found, and there were nonsignificant differences for HC. Posttest data showed significant correlations between all pairs of the selected exercises with the highest correlation between K2F jump height and VJ H (R = 0.40) followed by VJH and 2RM HC (R = 0.38) and 2RM HC and K2F jump height (R = 0.23). The results suggest that K2F jump training induced the desired learning effect but was specific to the movement in that no effect of transfer occurred to the other power-related movements. This finding is value for strength and condition professionals who design training programs to enhance athletic performance.

Preliminary results of thermal conductivity and elastic wave velocity measurements of various rock samples collected from outcrops in hanging wall of the Alpine Fault

NASA Astrophysics Data System (ADS)

Lin, W.; Tadai, O.; Shigematsu, N.; Nishikawa, O.; Mori, H.; Townend, J.; Capova, L.; Saito, S.; Kinoshita, M.

2015-12-01

The Alpine Fault is a mature active fault zone likely to rupture in the near future and DFDP aims to measure physical and chemical conditions within the fault. DFDP-2B borehole was drilled into hanging wall of the Alpine Fault. Downhole temperature measurements carried out in DFDP-2B borehole showed that the geothermal gradient in the hanging wall of the fault is very high, likely reaching to 130-150 °C/km (Sutherland et al., 2015 AGU Fall Meeting). To explain this abnormal feature, the determination of thermal properties of all the rock types in the hanging wall of the Alpine Fault is essential. To measure thermal properties and elastic wave velocities, we collected six typical rock block samples from outcrops in Stony creek and Gaunt creek. These include ultramylonite, mylonite, muscovite schist, garnet amphibolite, protomylonite and schist, which are representative of the hanging wall of the Alpine Fault. Their wet bulk densities are 2.7 - 2.8 g/cm3, and porosities are 1.4 - 3.0%. We prepared a pair of 4 cm cube specimens of each rock type with one flat plane parallel to the foliation. First, we measured thermal conductivity by the transient plane heat source (hot disc) method in a bulk mode, i.e. to deal with the rock as an isotropic material. However, several samples have clearly visible foliation and are likely to be anisotropic. Thus, the data measured in bulk mode provided an average value of the rocks in the range of approximately 2.4 - 3.2 W/mK. The next step will be to measure thermal conductivity in an anisotropic mode. We also measured P wave velocity (Vp) using the same samples, but in two directions, i.e. parallel and perpendicular to the foliation, respectively. Our preliminary results suggested that Vp is anisotropic in all the six rocks. Generally, Vp parallel to foliation is higher than that in the perpendicular direction. Vp in the parallel direction ranged in 5.5 - 6.0 km/s, whereas in the perpendicular direction it was 4.4 - 5.5 km/s. We

Laser scattering in a hanging drop vapor diffusion apparatus for protein crystal growth in a microgravity environment

NASA Technical Reports Server (NTRS)

Casay, G. A.; Wilson, W. W.

1992-01-01

One type of hardware used to grow protein crystals in the microgravity environment aboard the U.S. Space Shuttle is a hanging drop vapor diffusion apparatus (HDVDA). In order to optimize crystal growth conditions, dynamic control of the HDVDA is desirable. A critical component in the dynamically controlled system is a detector for protein nucleation. We have constructed a laser scattering detector for the HDVDA capable of detecting the nucleation stage. The detector was successfully tested for several scatterers differing in size using dynamic light scattering techniques. In addition, the ability to detect protein nucleation using the HDVDA was demonstrated for lysozyme.

Agonal sequences in 14 filmed hangings with comments on the role of the type of suspension, ischemic habituation, and ethanol intoxication on the timing of agonal responses.

PubMed

Sauvageau, Anny; Laharpe, Romano; King, David; Dowling, Graeme; Andrews, Sam; Kelly, Sean; Ambrosi, Corinne; Guay, Jean-Pierre; Geberth, Vernon J

2011-06-01

The Working Group on Human Asphyxia has analyzed 14 filmed hangings: 9 autoerotic accidents, 4 suicides, and 1 homicide. The following sequence of agonal responses was observed: rapid loss of consciousness in 10 ± 3 seconds, mild generalized convulsions in 14 ± 3 seconds, decerebrate rigidity in 19 ± 5 seconds, beginning of deep rhythmic abdominal respiratory movements in 19 ± 5 seconds, decorticate rigidity in 38 ± 15 seconds, loss of muscle tone in 1 minute 17 seconds ± 25 seconds, end of deep abdominal respiratory movements in 1 minute 51 seconds ± 30 seconds, and last muscle movement in 4 minutes 12 seconds ± 2 minutes 29 seconds. The type of suspension and ethanol intoxication does not seem to influence the timing of the agonal responses, whereas ischemic habituation in autoerotic practitioner might decelerate the late responses to hanging.

Wave Glider Monitoring of Sediment Transport and Dredge Plumes in a Shallow Marine Sandbank Environment.

PubMed

Van Lancker, Vera; Baeye, Matthias

2015-01-01

As human pressure on the marine environment increases, safeguarding healthy and productive seas increasingly necessitates integrated, time- and cost-effective environmental monitoring. Employment of a Wave Glider proved very useful for the study of sediment transport in a shallow sandbank area in the Belgian part of the North Sea. During 22 days, data on surface and water-column currents and turbidity were recorded along 39 loops around an aggregate-extraction site. Correlation with wave and tidal-amplitude data allowed the quantification of current- and wave-induced advection and resuspension, important background information to assess dredging impacts. Important anomalies in suspended particulate matter concentrations in the water column suggested dredging-induced overflow of sediments in the near field (i.e., dynamic plume), and settling of finer-grained material in the far field (i.e., passive plume). Capturing the latter is a successful outcome to this experiment, since the location of dispersion and settling of a passive plume is highly dependent on the ruling hydro-meteorological conditions and thus difficult to predict. Deposition of the observed sediment plumes may cause habitat changes in the long-term.

Wave Glider Monitoring of Sediment Transport and Dredge Plumes in a Shallow Marine Sandbank Environment

PubMed Central

Van Lancker, Vera; Baeye, Matthias

2015-01-01

As human pressure on the marine environment increases, safeguarding healthy and productive seas increasingly necessitates integrated, time- and cost-effective environmental monitoring. Employment of a Wave Glider proved very useful for the study of sediment transport in a shallow sandbank area in the Belgian part of the North Sea. During 22 days, data on surface and water-column currents and turbidity were recorded along 39 loops around an aggregate-extraction site. Correlation with wave and tidal-amplitude data allowed the quantification of current- and wave-induced advection and resuspension, important background information to assess dredging impacts. Important anomalies in suspended particulate matter concentrations in the water column suggested dredging-induced overflow of sediments in the near field (i.e., dynamic plume), and settling of finer-grained material in the far field (i.e., passive plume). Capturing the latter is a successful outcome to this experiment, since the location of dispersion and settling of a passive plume is highly dependent on the ruling hydro-meteorological conditions and thus difficult to predict. Deposition of the observed sediment plumes may cause habitat changes in the long-term. PMID:26070156

Seismic and gravity investigations of the shallow (upper 1 km) hanging wall of the Alpine Fault in the vicinity of the Whataroa River, New Zealand

NASA Astrophysics Data System (ADS)

Kovacs, A.; Gorman, A. R.; Lay, V.; Buske, S.

2013-12-01

Paleoseismic evidence from the vicinity of the plate-bounding Alpine Fault on New Zealand's South Island suggests that earthquakes of magnitude 7.9 occur every 200-400 years, with the last earthquake occurring in AD 1717. No human observations of this event are recorded. Therefore, the Deep Fault Drilling Project 2 (DFDP-2) drill hole, which is planned for 2014 on the hanging wall of the Alpine Fault in the Whataroa Valley, provides a critical opportunity to study the behavior of this transpressive plate boundary late in its seismogenic cycle. New seismic and gravity data collected since 2011 have been analyzed to assist with the positioning of the drill hole in this glacial valley that provides rare low-elevation access to the hanging wall of the Alpine Fault. The WhataDUSIE controlled-source seismic project, led by researchers from the University of Otago (New Zealand), TU Bergakademie Freiberg (Germany) and the University of Alberta (Canada), provided relatively high-resolution coverage (4-8 m geophone spacing, 25-100 m shot spacing) along a 5-km-long profile across the Alpine Fault in the Whataroa Valley. This work has been supplemented by focused hammer-seismic studies and gravity data collection in the valley. The former targets surface layer properties, whereas the latter targets the depth to the base of the glacially carved paleovalley. In positioning DFDP-2, an understanding of the nature of overburden and valley-fill sediments is critical for drilling design. A velocity model has been developed for the valley based on refraction analysis of the WhataDUSIE and hammer-seismic data combined with a ray-theoretical travel-time tomography (RAYINVR) image of the shallow (uppermost 1 km or so) part of the hanging wall of the Alpine Fault. The model shows that the glacial valley, which presumably was last scoured by ice at the Last Glacial Maximum, has been filled with 200-350 m of post-glacial sediments and outwash gravels. The hanging-wall rocks into which the

Hanging drop cultures of human testis and testis cancer samples: a model used to investigate activin treatment effects in a preserved niche.

PubMed

Jørgensen, A; Young, J; Nielsen, J E; Joensen, U N; Toft, B G; Rajpert-De Meyts, E; Loveland, K L

2014-05-13

Testicular germ cell tumours of young adults, seminoma or non-seminomas, are preceded by a pre-invasive precursor, carcinoma in situ (CIS), understood to arise through differentiation arrest of embryonic germ cells. Knowledge about the malignant transformation of germ cells is currently limited by the lack of experimental models. The aim of this study was to establish an experimental tissue culture model to maintain normal and malignant germ cells within their niche and allow investigation of treatment effects. Human testis and testis cancer specimens from orchidectomies were cultured in 'hanging drops' and effects of activin A and follistatin treatment were investigated in seminoma cultures. Testis fragments with normal spermatogenesis or CIS cells were cultured for 14 days with sustained proliferation of germ cells and CIS cells and without increased apoptosis. Seminoma cultures survived 7 days, with proliferating cells detectable during the first 5 days. Activin A treatment significantly reduced KIT transcript and protein levels in seminoma cultures, thereby demonstrating a specific treatment response. Hanging drop cultures of human testis and testis cancer samples can be employed to delineate mechanisms governing growth of normal, CIS and tumorigenic germ cells retained within their niche.

Hanging drop cultures of human testis and testis cancer samples: a model used to investigate activin treatment effects in a preserved niche

PubMed Central

Jørgensen, A; Young, J; Nielsen, J E; Joensen, U N; Toft, B G; Rajpert-De Meyts, E; Loveland, K L

2014-01-01

Background: Testicular germ cell tumours of young adults, seminoma or non-seminomas, are preceded by a pre-invasive precursor, carcinoma in situ (CIS), understood to arise through differentiation arrest of embryonic germ cells. Knowledge about the malignant transformation of germ cells is currently limited by the lack of experimental models. The aim of this study was to establish an experimental tissue culture model to maintain normal and malignant germ cells within their niche and allow investigation of treatment effects. Methods: Human testis and testis cancer specimens from orchidectomies were cultured in ‘hanging drops' and effects of activin A and follistatin treatment were investigated in seminoma cultures. Results: Testis fragments with normal spermatogenesis or CIS cells were cultured for 14 days with sustained proliferation of germ cells and CIS cells and without increased apoptosis. Seminoma cultures survived 7 days, with proliferating cells detectable during the first 5 days. Activin A treatment significantly reduced KIT transcript and protein levels in seminoma cultures, thereby demonstrating a specific treatment response. Conclusions: Hanging drop cultures of human testis and testis cancer samples can be employed to delineate mechanisms governing growth of normal, CIS and tumorigenic germ cells retained within their niche. PMID:24781282

Glider and satellite high resolution monitoring of a mesoscale eddy in the Algerian basin: effects on the mixed layer depth and biochemistry

NASA Astrophysics Data System (ADS)

Cotroneo, Yuri; Aulicino, Giuseppe; Ruiz, Simón; Pascual, Ananda; Budillon, Giorgio; Fusco, Giannetta; Tintoré, Joaquin

2016-04-01

Despite of the extensive bibliography about the circulation of the Mediterranean Sea and its sub-basins, the debate on mesoscale dynamics and its impacts on biochemical processes is still open because of their intrinsic time scales and of the difficulties in sampling. In order to clarify some of these processes, the "Algerian BAsin Circulation Unmanned Survey - ABACUS" project was proposed and realized through access to JERICO Trans National Access (TNA) infrastructures between September and December 2014. In this framework, a deep glider cruise was carried out in the area between Balearic Islands and Algerian coasts to establish an endurance line for monitoring the basin circulation. During the mission, a mesoscale eddy, identified on satellite altimetry maps, was sampled at high-spatial horizontal resolution (4 km) along its main axes and from surface to 1000 m depth. Data were collected by a Slocum glider equipped with a pumped CTD and biochemical sensors that collected about 100 complete casts inside the eddy. In order to describe the structure of the eddy, in situ data were merged with new generation remotely sensed data as daily synoptic sea surface temperature (SST) and chlorophyll concentration (Chl-a) images from MODIS satellites as well as sea surface height and geostrophic velocities from AVISO. From its origin along the Algerian coast in the eastern part of the basin, the eddy propagated to north-west at a mean speed of about 4 km/day with a mean diameter of 112/130 km, a mean elevation of 15.7 cm and clearly distinguished by the surrounding waters thanks to its higher SST and Chl-a values. Temperature and salinity values along the water column confirm the origin of the eddy from the AC showing the presence of recent Atlantic water in the surface layer and Levantine Intermediate Water (LIW) in the deeper layer. Eddy footprint is clearly evident in the multiparametric vertical sections conducted along its main axes. Deepening of temperature, salinity and

Blooms and subsurface phytoplankton layers on the Scotian Shelf: Insights from profiling gliders

NASA Astrophysics Data System (ADS)

Ross, Tetjana; Craig, Susanne E.; Comeau, Adam; Davis, Richard; Dever, Mathieu; Beck, Matthew

2017-08-01

Understanding how phytoplankton respond to their physical environment is key to predicting how bloom dynamics might change under future climate change scenarios. Phytoplankton are at the base of most marine food webs and play an important role in drawing CO2 out of the atmosphere. Using nearly 5 years of simultaneous CTD, irradiance, chlorophyll a fluorescence and optical backscattering observations obtained from Slocum glider missions, we observed the subsurface phytoplankton populations across the Scotian Shelf, near Halifax (Nova Scotia, Canada) along with their physical environment. Bloom conditions were observed in each of the 5 springs, with the average chlorophyll in the upper 60 m of water generally exceeding 3 mg m- 3. These blooms occurred when the upper water column stratification was at its lowest, in apparent contradiction of the critical depth hypothesis. A subsurface chlorophyll layer was observed each summer at about 30 m depth, which was below the base of the mixed layer. This subsurface layer lasted 3-4 months and contained, on average, 1/4 of the integrated water column chlorophyll found during the spring bloom. This suggests that a significant portion of the primary productivity over the Scotian Shelf occurs at depths that cannot be observed by satellites-highlighting the importance of including subsurface observations in the monitoring of future changes to primary productivity in the ocean.

Spreading of Levantine Intermediate Waters by submesoscale coherent vortices in the northwestern Mediterranean Sea as observed with gliders

NASA Astrophysics Data System (ADS)

Bosse, Anthony; Testor, Pierre; Mortier, Laurent; Prieur, Louis; Taillandier, Vincent; d'Ortenzio, Fabrizio; Coppola, Laurent

2015-03-01

Since 2007, gliders have been regularly deployed in the northwestern Mediterranean Sea, a crucial region regarding the thermohaline circulation of the Mediterranean Sea. It revealed for the first time very warm (+0.4∘C) and saline (+0.1) submesoscale anticyclones at intermediate depth characterized by a small radius (˜5 km), high Rossby (˜0.3), and Burger (˜0.7) numbers. They are likely order of 10 to be formed each year, have a life time order a year and certainly contribute significantly to the spreading of the Levantine Intermediate Waters (LIW) toward the whole subbasin, thus potentially impacting wintertime vertical mixing through hydrographical and dynamical preconditioning. They could be mainly formed by the combined action of turbulent mixing and flow detachment of the northward flow of LIW at the northwestern headland of Sardinia. Upwelling conditions along the western coast of Sardinia associated with a southward geostrophic flow within the upper layers seem to play a key role in their formation process.

Etiology and use of the "hanging drop" technique: a review.

PubMed

Todorov, Ludmil; VadeBoncouer, Timothy

2014-01-01

Background. The hanging drop (HD) technique presumably relies on the presence of subatmospheric epidural pressure. It is not clear whether this negative pressure is intrinsic or an artifact and how it is affected by body position. There are few data to indicate how often HD is currently being used. Methods. We identified studies that measured subatmospheric pressures and looked at the effect of the sitting position. We also looked at the technique used for cervical and thoracic epidural anesthesia in the last 10 years. Results. Intrinsic subatmospheric pressures were measured in the thoracic and cervical spine. Three trials studied the effect of body position, indicating a higher incidence of subatmospheric pressures when sitting. The results show lower epidural pressure (-10.7 mmHg) with the sitting position. 28.8% of trials of cervical and thoracic epidural anesthesia that documented the technique used, utilized the HD technique. When adjusting for possible bias, the rate of HD use can be as low as 11.7%. Conclusions. Intrinsic negative pressure might be present in the cervical and thoracic epidural space. This effect is more pronounced when sitting. This position might be preferable when using HD. Future studies are needed to compare it with the loss of resistance technique.

SU-E-T-556: Monte Carlo Generated Dose Distributions for Orbital Irradiation Using a Single Anterior-Posterior Electron Beam and a Hanging Lens Shield

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duwel, D; Lamba, M; Elson, H

Purpose: Various cancers of the eye are successfully treated with radiotherapy utilizing one anterior-posterior (A/P) beam that encompasses the entire content of the orbit. In such cases, a hanging lens shield can be used to spare dose to the radiosensitive lens of the eye to prevent cataracts. Methods: This research focused on Monte Carlo characterization of dose distributions resulting from a single A-P field to the orbit with a hanging shield in place. Monte Carlo codes were developed which calculated dose distributions for various electron radiation energies, hanging lens shield radii, shield heights above the eye, and beam spoiler configurations.more » Film dosimetry was used to benchmark the coding to ensure it was calculating relative dose accurately. Results: The Monte Carlo dose calculations indicated that lateral and depth dose profiles are insensitive to changes in shield height and electron beam energy. Dose deposition was sensitive to shield radius and beam spoiler composition and height above the eye. Conclusion: The use of a single A/P electron beam to treat cancers of the eye while maintaining adequate lens sparing is feasible. Shield radius should be customized to have the same radius as the patient’s lens. A beam spoiler should be used if it is desired to substantially dose the eye tissues lying posterior to the lens in the shadow of the lens shield. The compromise between lens sparing and dose to diseased tissues surrounding the lens can be modulated by varying the beam spoiler thickness, spoiler material composition, and spoiler height above the eye. The sparing ratio is a metric that can be used to evaluate the compromise between lens sparing and dose to surrounding tissues. The higher the ratio, the more dose received by the tissues immediately posterior to the lens relative to the dose received by the lens.« less

Formation and hematopoietic differentiation of human embryoid bodies by suspension and hanging drop cultures.

PubMed

Cerdan, Chantal; Hong, Seok Ho; Bhatia, Mickie

2007-10-01

The in vitro aggregation of human embryonic stem cells (hESCs) into clusters termed embryoid bodies (EBs) allows for the spontaneous differentiation of cells representing endoderm, mesoderm, and ectoderm lineages. This stochastic process results however, in the generation of low numbers of differentiated cells, and can be enhanced to some extent by the addition of exogenous growth factors or overexpression of regulatory genes. In the authors' laboratory, the use of hematopoietic cytokines in combination with the mesoderm inducer bone morphogenetic protein-4 (BMP-4) was able to generate up to 90% of CD45(+) hematopoietic cells with colony-forming unit (CFU) activity. This unit describes two protocols that have been successfully applied in the authors' laboratory for the generation of EBs in (1) suspension and (2) hanging drop (HD) cultures from enzymatically digested clumps of undifferentiated hESC colonies.

Studying electron transfer through alkanethiol self-assembled monolayers on a hanging mercury drop electrode using potentiometric measurements.

PubMed

Cohen-Atiya, Meirav; Mandler, Daniel

2006-10-14

A new approach based on measuring the change of the open-circuit potential (OCP) of a hanging mercury drop electrode (HMDE), modified with alkanethiols of different chain length conducted in a solution containing a mixture of Ru(NH3)6(2+) and Ru(NH3)6(3+) is used for studying electron transfer across the monolayer. Following the time dependence of the OCP allowed the extraction of the kinetic parameters, such as the charge transfer resistance (R(ct)) and the electron transfer rate constant (k(et)), for different alkanethiol monolayers. An electron tunneling coefficient, beta, of 0.9 A(-1) was calculated for the monolayers on Hg.

A Blanik L-23 glider carrying a microphone and a pressure transducer flies near a BADS sensor following flight under the path of the F-5E SSBE aircraft

NASA Image and Video Library

2004-01-13

A United States Air Force Test Pilot School Blanik L-23 glider carrying a microphone and a pressure transducer flies near a BADS (Boom Amplitudes Direction System) sensor following flight at an altitude of 10 thousand feet under the path of the F-5E SSBE aircraft. The SSBE (Shaped Sonic Boom Experiment) was formerly known as the Shaped Sonic Boom Demonstration, or SSBD, and is part of DARPA's Quiet Supersonic Platform (QSP) program. On August 27, 2003, the F-5E SSBD aircraft demonstrated a method to reduce the intensity of sonic booms.

Aerodynamic Performance and Static Stability and Control of Flat-Top Hypersonic Gliders at Mach Numbers from 0.6 to 18

NASA Technical Reports Server (NTRS)

Syvertson, Clarence A; Gloria, Hermilo R; Sarabia, Michael F

1958-01-01

A study is made of aerodynamic performance and static stability and control at hypersonic speeds. In a first part of the study, the effect of interference lift is investigated by tests of asymmetric models having conical fuselages and arrow plan-form wings. The fuselage of the asymmetric model is located entirely beneath the wing and has a semicircular cross section. The fuselage of the symmetric model was centrally located and has a circular cross section. Results are obtained for Mach numbers from 3 to 12 in part by application of the hypersonic similarity rule. These results show a maximum effect of interference on lift-drag ratio occurring at Mach number of 5, the Mach number at which the asymmetric model was designed to exploit favorable lift interference. At this Mach number, the asymmetric model is indicated to have a lift-drag ratio 11 percent higher than the symmetric model and 15 percent higher than the asymmetric model when inverted. These differences decrease to a few percent at a Mach number of 12. In the course of this part of the study, the accuracy to the hypersonic similarity rule applied to wing-body combinations is demonstrated with experimental results. These results indicate that the rule may prove useful for determining the aerodynamic characteristics of slender configurations at Mach numbers higher than those for which test equipment is really available. In a second part of the study, the aerodynamic performance and static stability and control characteristics of a hypersonic glider are investigated in somewhat greater detail. Results for Mach numbers from 3 to 18 for performance and 0.6 to 12 for stability and control are obtained by standard text techniques, by application of the hypersonic stability rule, and/or by use of helium as a test medium. Lift-drag ratios of about 5 for Mach numbers up to 18 are shown to be obtainable. The glider studied is shown to have acceptable longitudinal and directional stability characteristics through the

Roof Weakening of Hydraulic Fracturing for Control of Hanging Roof in the Face End of High Gassy Coal Longwall Mining: A Case Study

NASA Astrophysics Data System (ADS)

Huang, Bingxiang; Wang, Youzhuang

2016-09-01

The occurence of hanging roof commonly arises in the face end of longwall coal mining under hard roof conditions. The sudden break and subsequent caving of a hanging roof could result in the extrusion of gas in the gob to the face, causing gas concentrations to rise sharply and to increase to over a safety-limited value. A series of linear fracturing-holes of 32 mm diameter were drilled into the roof of the entries with an anchor rig. According to the theory that the gob should be fully filled with the fragmentized falling roof rock, the drilling depth is determined as being 3 5 times the mining height if the broken expansion coefficient takes an empirical value. Considering the general extension range of cracks and the supporting form of the entryway, the spacing distance between two drilling holes is determined as being 1 2 times the crack's range of extension. Using a mounting pipe, a high pressure resistant sealing device of a small diameter-size was sent to the designated location for the high-pressure hydraulic fracturing of the roof rock. The hydraulic fracturing created the main hydro-fracturing crack and airfoil branch cracks in the interior of the roof-rock, transforming the roof structure and weakening the strength of the roof to form a weak plane which accelerated roof caving, and eventually induced the full caving in of the roof in time with the help of ground pressure. For holes deeper than 4 m, retreating hydraulic fracturing could ensure the uniformity of crack extension. Tested and applied at several mines in Shengdong Mining District, the highest ruptured water pressure was found to be 55 MPa, and the hanging roof at the face end was reduced in length from 12 m to less than 1 2 m. This technology has eliminated the risk of the extrusion of gas which has accumulated in the gob.

Changes in Suicide Rates by Hanging/Suffocation and Firearms among Young Persons Aged 10 to 24 Years in the United States: 1992–2006

PubMed Central

Bridge, Jeffrey A.; Greenhouse, Joel B.; Sheftall, Arielle H.; Fabio, Anthony; Campo, John V.; Kelleher, Kelly J.

2009-01-01

We examined changes in suicide rates among 10–24-year-olds in the US from 1992–2006. The overall suicide rate and the rate by firearms, poisoning, and other methods declined markedly, whereas the hanging/suffocation rate rose significantly from 1992–2006. This increase occurred across every major demographic subgroup, but was most dramatic for females. PMID:20413089

Satellite and in situ measurements for coastal water quality assessment and monitoring: a comparison between MODIS Ocean Color and SST products with Wave Glider observations in the Southern Tyrrhenian Sea (Gulf of Naples, Italy).

NASA Astrophysics Data System (ADS)

Sileo, Giancanio; Lacava, Teodosio; Tramutoli, Valerio; Budillon, Giorgio; Aulicino, Giuseppe; Cotroneo, Yuri; Ciancia, Emanuele; De Stefano, Massimo; Fusco, Giannetta; Pergola, Nicola; Satriano, Valeria

2015-04-01

A wave-propelled autonomous vehicle (Wave Glider, WG) carrying a variety of oceanographic and meteorological sensors was launched from Gulf of Naples on the 12th September 2012 for a three-week mission in the Southern Tyrrhenian Sea. The main objective of the mission was the opportunity to evaluate the usefulness of combined satellite and autonomous platform observations in providing reliable and concurrent information about sea water parameters about the Southern Tyrrhenian Sea surface layer. The Wave Glider was equipped with sensors to measure temperature, salinity, currents, as well as CDOM, turbidity and refined fuels fluorescence. Wave Glider oceanographic data were also compared to satellite measurements. In particular, MODIS Ocean Color (OC) products concerning sea water properties collected during the Wave Glider mission were used. The EOS constellation allowed us to have about two daily diurnal imagery providing information about ocean color products. Concerning SST, both diurnal and night-time data were available. The first study we performed was focused on the analysis of SST information coming from both WG and MODIS. A good coefficient of correlation was achieved considering together both day-time and night-time acquisitions, with a discrepancy not higher than 0,7 °C. The correlation increases considering only day-time values, when more samples respect to the night-time ones were available. The results confirm the capability of MODIS products to reproduce over large area the SST variability, with a good level of accuracy. A similar analysis has been carried out to compare the turbidity WG data with the kd-490 MODIS product, which provide information about the diffuse attenuation coefficient in water at 490 nm and it is directly related to the presence of scattering particles, either organic or inorganic, in the water column and thus it is an indication of water clarity or of the water column turbidity. The absence of correlation seems to indicate, for

Etiology and Use of the “Hanging Drop” Technique: A Review

PubMed Central

Todorov, Ludmil; VadeBoncouer, Timothy

2014-01-01

Background. The hanging drop (HD) technique presumably relies on the presence of subatmospheric epidural pressure. It is not clear whether this negative pressure is intrinsic or an artifact and how it is affected by body position. There are few data to indicate how often HD is currently being used. Methods. We identified studies that measured subatmospheric pressures and looked at the effect of the sitting position. We also looked at the technique used for cervical and thoracic epidural anesthesia in the last 10 years. Results. Intrinsic subatmospheric pressures were measured in the thoracic and cervical spine. Three trials studied the effect of body position, indicating a higher incidence of subatmospheric pressures when sitting. The results show lower epidural pressure (−10.7 mmHg) with the sitting position. 28.8% of trials of cervical and thoracic epidural anesthesia that documented the technique used, utilized the HD technique. When adjusting for possible bias, the rate of HD use can be as low as 11.7%. Conclusions. Intrinsic negative pressure might be present in the cervical and thoracic epidural space. This effect is more pronounced when sitting. This position might be preferable when using HD. Future studies are needed to compare it with the loss of resistance technique. PMID:24839558

Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture.

PubMed

Schmal, Olga; Seifert, Jan; Schäffer, Tilman E; Walter, Christina B; Aicher, Wilhelm K; Klein, Gerd

2016-01-01

Efficient ex vivo expansion of hematopoietic stem cells with a concomitant preservation of stemness and self-renewal potential is still an unresolved ambition. Increased numbers of methods approaching this issue using three-dimensional (3D) cultures were reported. Here, we describe a simplified 3D hanging drop model for the coculture of cord blood-derived CD34(+) hematopoietic stem and progenitor cells (HSPCs) with bone marrow-derived mesenchymal stromal cells (MSCs). When seeded as a mixed cell suspension, MSCs segregated into tight spheroids. Despite the high expression of niche-specific extracellular matrix components by spheroid-forming MSCs, HSPCs did not migrate into the spheroids in the initial phase of coculture, indicating strong homotypic interactions of MSCs. After one week, however, HSPC attachment increased considerably, leading to spheroid collapse as demonstrated by electron microscopy and immunofluorescence staining. In terms of HSPC proliferation, the conventional 2D coculture system was superior to the hanging drop model. Furthermore, expansion of primitive hematopoietic progenitors was more favored in 2D than in 3D, as analyzed in colony-forming assays. Conclusively, our data demonstrate that MSCs, when arranged with a spread (monolayer) shape, exhibit better HSPC supportive qualities than spheroid-forming MSCs. Therefore, 3D systems are not necessarily superior to traditional 2D culture in this regard.

A hollow sphere soft lithography approach for long-term hanging drop methods.

PubMed

Lee, Won Gu; Ortmann, Daniel; Hancock, Matthew J; Bae, Hojae; Khademhosseini, Ali

2010-04-01

In conventional hanging drop (HD) methods, embryonic stem cell aggregates or embryoid bodies (EBs) are often maintained in small inverted droplets. Gravity limits the volumes of these droplets to less than 50 microL, and hence such cell cultures can only be sustained for a few days without frequent media changes. Here we present a new approach to performing long-term HD methods (10-15 days) that can provide larger media reservoirs in a HD format to maintain more consistent culture media conditions. To implement this approach, we fabricated hollow sphere (HS) structures by injecting liquid drops into noncured poly(dimethylsiloxane) mixtures. These structures served as cell culture chambers with large media volumes (500 microL in each sphere) where EBs could grow without media depletion. The results showed that the sizes of the EBs cultured in the HS structures in a long-term HD format were approximately twice those of conventional HD methods after 10 days in culture. Further, HS cultures showed multilineage differentiation, similar to EBs cultured in the HD method. Due to its ease of fabrication and enhanced features, this approach may be of potential benefit as a stem cell culture method for regenerative medicine.

Thermoluminesence Properties and Ages along the Stony Creek, Hanging Wall of Alpine Fault, New Zealand

NASA Astrophysics Data System (ADS)

Nishikawa, O.; Theeraporn, C.; Takashima, I.; Shigematsu, N.; Little, T. A.; Boulton, C. J.

2015-12-01

The Alpine Fault, New Zealand is an oblique slip thrust with significantly high slip rate, and its dip-slip component causes the rapid uplift of the Southern Alps and the extremely high geothermal gradient in it. Thermoluminescence (TL) dating is a method using the phenomenon that energy accumulated in the crystal from radiation of surrounding radioactive elements is reemitted in the form of light when heating the minerals. This method covers a wide range of age from 1,000 to 1,000,000 years, and has relatively low reset temperature for the accumulation of radiation dose. Therefore, TL dating is a feasible geochronometry for the reconstruction of the thermal history of the area with very high uplifting rate. In order to determine uplifting rates and their distribution in the Southern Alps adjacent to the Alpine fault, ten rock samples were collected for TL dating in the distance 1 km from main fault plane along the Stony Creek. All the samples commonly include quartz veins which are folded tightly or in isoclinal form parallel to the foliations. TL dating was performed using quartz grains separated from host rock. A widely ranging TL ages are obtained from the hanging wall of the fault. The rocks within 600m from present shear zone yield ages ranging from 55.2 ka to 88.8 ka, showing older ages with distance from shear zone. Within 600 m to 900 m from the fault, relatively younger ages, 54.7 to 34.4 ka are obtained. Assuming the thermal gradient of 10 °C /100 m and exhumation rate of 10 m / kyr, the zeroing depth and temperature of TL signals is estimated from 350 to 900 m and from 45 to 100 °C, respectively. The range of TL ages is very large amounted to 50,000 years in the narrow zone. This may be responsible for the variety of TL zeroing temperatures in the hanging wall rocks rather than disturbance of thermal structure and/or inhomogeneity of uplifting rate in this area. Annealing tests are necessary to clarify the real properties of TL for each sample tested.

14 CFR 61.23 - Medical certificates: Requirement and duration.

Code of Federal Regulations, 2011 CFR

2011-01-01

... pilot certificate with glider or balloon privileges; or (ii) A pilot certificate with a glider category... privileges in a glider or balloon; (3) When exercising the privileges of a pilot certificate with a glider category rating or balloon class rating in a glider or a balloon, as appropriate; (4) When exercising the...

14 CFR 61.23 - Medical certificates: Requirement and duration.

Code of Federal Regulations, 2012 CFR

2012-01-01

... pilot certificate with glider or balloon privileges; or (ii) A pilot certificate with a glider category... privileges in a glider or balloon; (3) When exercising the privileges of a pilot certificate with a glider category rating or balloon class rating in a glider or a balloon, as appropriate; (4) When exercising the...