40 CFR Table 4 to Subpart Rrrr of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups 1

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants...

40 CFR Table 5 to Subpart Oooo of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Printing, Coating...

40 CFR Table 4 to Subpart Mmmm of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants for...

40 CFR Table 3 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants for...

40 CFR Table 4 to Subpart Nnnn of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants...

40 CFR Table 3 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants for...

40 CFR Table 4 to Subpart Mmmm of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants for...

40 CFR Table 4 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants for...

40 CFR Table 3 to Subpart IIIi of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants...

40 CFR Table 5 to Subpart Oooo of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants...

40 CFR Table 4 to Subpart Oooo of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants...

40 CFR Table 6 to Subpart Qqqq of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants...

40 CFR Table 3 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants for...

40 CFR Table 4 to Subpart Rrrr of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups 1

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 4 to Subpart IIIi of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 6 to Subpart Qqqq of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 4 to Subpart Mmmm of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 7 to Subpart Kkkk of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 4 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 3 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 4 to Subpart Rrrr of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups 1

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 3 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 4 to Subpart Nnnn of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 4 to Subpart Pppp of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 4 to Subpart IIIi of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 4 to Subpart Mmmm of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 7 to Subpart Kkkk of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 6 to Subpart Qqqq of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR Table 3 to Subpart Mmmm of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 3 to Subpart Mmmm of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Surface Coating...

40 CFR Table 4 to Subpart Nnnn of... - Default Organic HAP Mass Fraction for Petroleum Solvent Groups a

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Default Organic HAP Mass Fraction for... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants: Surface Coating of...

40 CFR 63.5335 - How do I determine the actual HAP loss?

Code of Federal Regulations, 2011 CFR

2011-07-01

... to the leather; (iii) Mass fraction of HAP in each applied finish; (iv) Date of the recorded entry... recorded finish usage by the corresponding mass fraction of HAP in the finish. The result is the HAP loss... the pounds of each recorded finish usage by the corresponding mass fraction of HAP in the finish. The...

40 CFR 63.5335 - How do I determine the actual HAP loss?

Code of Federal Regulations, 2013 CFR

2013-07-01

... and the mass fraction of HAP in each solvent/finish. (1) Measure Finish as Applied. Use a finish... and the mass fraction of HAP in each applied finish. Figure 1 of this subpart shows an example log for... each finish applied to the leather; (iii) Mass fraction of HAP in each applied finish; (iv) Date of the...

40 CFR 63.5335 - How do I determine the actual HAP loss?

Code of Federal Regulations, 2012 CFR

2012-07-01

... and the mass fraction of HAP in each solvent/finish. (1) Measure Finish as Applied. Use a finish... and the mass fraction of HAP in each applied finish. Figure 1 of this subpart shows an example log for... each finish applied to the leather; (iii) Mass fraction of HAP in each applied finish; (iv) Date of the...

40 CFR 63.5335 - How do I determine the actual HAP loss?

Code of Federal Regulations, 2014 CFR

2014-07-01

... and the mass fraction of HAP in each solvent/finish. (1) Measure Finish as Applied. Use a finish... and the mass fraction of HAP in each applied finish. Figure 1 of this subpart shows an example log for... each finish applied to the leather; (iii) Mass fraction of HAP in each applied finish; (iv) Date of the...

40 CFR 63.5758 - How do I determine the organic HAP content of materials?

Code of Federal Regulations, 2011 CFR

2011-07-01

... Method 311 for determining the mass fraction of organic HAP. Use the procedures specified in paragraphs... in the organic HAP total. Express the mass fraction of each organic HAP you measure as a value...). You may use Method 24 to determine the mass fraction of non-aqueous volatile matter of aluminum...

40 CFR 63.5758 - How do I determine the organic HAP content of materials?

Code of Federal Regulations, 2012 CFR

2012-07-01

... part 63). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures... include it in the organic HAP total. Express the mass fraction of each organic HAP you measure as a value...). You may use Method 24 to determine the mass fraction of non-aqueous volatile matter of aluminum...

40 CFR 63.5758 - How do I determine the organic HAP content of materials?

Code of Federal Regulations, 2014 CFR

2014-07-01

... part 63). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures... include it in the organic HAP total. Express the mass fraction of each organic HAP you measure as a value...). You may use Method 24 to determine the mass fraction of non-aqueous volatile matter of aluminum...

40 CFR 63.5758 - How do I determine the organic HAP content of materials?

Code of Federal Regulations, 2013 CFR

2013-07-01

... part 63). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures... include it in the organic HAP total. Express the mass fraction of each organic HAP you measure as a value...). You may use Method 24 to determine the mass fraction of non-aqueous volatile matter of aluminum...

40 CFR 63.8055 - How do I comply with a weight percent HAP limit in coating products?

Code of Federal Regulations, 2012 CFR

2012-07-01

... (appendix A to 40 CFR part 60). You may use Method 24 to determine the mass fraction of volatile matter and use that value as a substitute for the mass fraction of HAP. (3) You may use an alternative test method for determining mass fraction of HAP if you obtain prior approval by the Administrator. You must...

40 CFR 63.8055 - How do I comply with a weight percent HAP limit in coating products?

Code of Federal Regulations, 2014 CFR

2014-07-01

... (appendix A to 40 CFR part 60). You may use Method 24 to determine the mass fraction of volatile matter and use that value as a substitute for the mass fraction of HAP. (3) You may use an alternative test method for determining mass fraction of HAP if you obtain prior approval by the Administrator. You must...

40 CFR 63.8055 - How do I comply with a weight percent HAP limit in coating products?

Code of Federal Regulations, 2013 CFR

2013-07-01

... (appendix A to 40 CFR part 60). You may use Method 24 to determine the mass fraction of volatile matter and use that value as a substitute for the mass fraction of HAP. (3) You may use an alternative test method for determining mass fraction of HAP if you obtain prior approval by the Administrator. You must...

40 CFR 63.8055 - How do I comply with a weight percent HAP limit in coating products?

Code of Federal Regulations, 2011 CFR

2011-07-01

... (appendix A to 40 CFR part 60). You may use Method 24 to determine the mass fraction of volatile matter and use that value as a substitute for the mass fraction of HAP. (3) You may use an alternative test method for determining mass fraction of HAP if you obtain prior approval by the Administrator. You must...

40 CFR Figure 1 to Subpart Tttt of... - Example Logs for Recording Leather Finish Use and HAP Content

Code of Federal Regulations, 2010 CFR

2010-07-01

... Finishing Operations Part 63, Subpt. TTTT, Fig. 1 Figure 1 to Subpart TTTT of Part 63—Example Logs for Recording Leather Finish Use and HAP Content Month:______Year:______ Finish Inventory Log Finish type Finish usage(pounds) HAP Content(mass fraction) Date and time Operator's name Product process operation Monthly...

Mechanical properties and corrosion behavior of Mg-HAP composites.

PubMed

Campo, R Del; Savoini, B; Muñoz, A; Monge, M A; Garcés, G

2014-11-01

Mg and Mg-HAP composites containing 5, 10 and 15 wt% of hydroxyapatite have been produced following a powder metallurgy route that consists of mixing raw powders and consolidation by extrusion. The microstructure, texture, mechanical behavior and resistance to corrosion under a PBS solution have been studied. Addition of HAP increases the microhardness of the composites, however the yield strength under compression slightly decreases. Texture analyses reveal a fiber texture for pure Mg that is weakened increasing the HAP fraction. This texture promotes twinning and softening of Mg and Mg-5HAP during the initial deformation stages. Mg-10HAP and Mg-15HAP present a strain-hardening dependence showing no softening. The volume fraction of HAP particles weakens the texture and favors the activation of secondary slip systems. Corrosion experiments in PBS solution have shown that Mg-5HAP exhibits the best resistance to corrosion. Texture and porosity appear to be the main material features controlling the corrosion rates of Mg-HAP composites under the present conditions. Copyright © 2014 Elsevier Ltd. All rights reserved.

40 CFR 63.5749 - How do I calculate the organic HAP content of aluminum wipedown solvents?

Code of Federal Regulations, 2011 CFR

2011-07-01

... the past 12 months, liters. Dj= density of aluminum wipedown solvent j, kilograms per liter. Wj= mass fraction of organic HAP in aluminum wipedown solvent j. m = number of different aluminum surface coatings...

40 CFR 63.4151 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... option. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of... coating per liter coating. Wc,i = mass fraction of organic HAP in coating, i, kg organic HAP per kg... thinner per liter thinner. Wt,j = mass fraction of organic HAP in thinner, j, kg organic HAP per kg...

40 CFR 63.4151 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... option. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of... coating per liter coating. Wc,i = mass fraction of organic HAP in coating, i, kg organic HAP per kg... thinner per liter thinner. Wt,j = mass fraction of organic HAP in thinner, j, kg organic HAP per kg...

HapZipper: sharing HapMap populations just got easier.

PubMed

Chanda, Pritam; Elhaik, Eran; Bader, Joel S

2012-11-01

The rapidly growing amount of genomic sequence data being generated and made publicly available necessitate the development of new data storage and archiving methods. The vast amount of data being shared and manipulated also create new challenges for network resources. Thus, developing advanced data compression techniques is becoming an integral part of data production and analysis. The HapMap project is one of the largest public resources of human single-nucleotide polymorphisms (SNPs), characterizing over 3 million SNPs genotyped in over 1000 individuals. The standard format and biological properties of HapMap data suggest that a dedicated genetic compression method can outperform generic compression tools. We propose a compression methodology for genetic data by introducing HapZipper, a lossless compression tool tailored to compress HapMap data beyond benchmarks defined by generic tools such as gzip, bzip2 and lzma. We demonstrate the usefulness of HapZipper by compressing HapMap 3 populations to <5% of their original sizes. HapZipper is freely downloadable from https://bitbucket.org/pchanda/hapzipper/downloads/HapZipper.tar.bz2.

40 CFR 63.3531 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... fraction of organic HAP for each material. Determine the mass fraction of organic HAP for each coating and... coating. Wc,i = Mass fraction of organic HAP in coating, i, kg organic HAP per kg coating. m = Number of...,j = Density of thinner, j, kg per liter. Wt,j = Mass fraction of organic HAP in thinner, j, kg...

40 CFR 63.3531 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... fraction of organic HAP for each material. Determine the mass fraction of organic HAP for each coating and... coating. Wc,i = Mass fraction of organic HAP in coating, i, kg organic HAP per kg coating. m = Number of...,j = Density of thinner, j, kg per liter. Wt,j = Mass fraction of organic HAP in thinner, j, kg...

40 CFR 63.3531 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2013 CFR

2013-07-01

... fraction of organic HAP for each material. Determine the mass fraction of organic HAP for each coating and... coating. Wc,i = Mass fraction of organic HAP in coating, i, kg organic HAP per kg coating. m = Number of...,j = Density of thinner, j, kg per liter. Wt,j = Mass fraction of organic HAP in thinner, j, kg...

Process-based control of HAPs emissions from drying wood flakes.

PubMed

Banerjee, Sujit; Pendyala, Krishna; Buchanan, Mike; Yang, Rallming; Abu-Daabes, Malyuba; Otwell, Lawrence P E

2006-04-01

Industrial wood flake drying generates methanol, formaldehyde, and other hazardous air pollutants (HAPs). A simple theoretical model shows that particles smaller than 400 microm will begin to thermally degrade and release disproportionately large quantities of HAPs. This is confirmed in full-scale practice where particles smaller than 500 microm show visible signs of charring. Laboratory measurement of the activation energy for the breakdown of wood tissue into methanol and formaldehyde led to a value of about 17 kcal/mol. The apparent activation energy measured in the field was higher. This result was obtained under nonisothermal conditions and is biased high by the fines fraction of the furnish, which is exposed to elevated temperatures. It is proposed that a combination of screening out the fines fraction smaller than 500 microm and reducing the dryer inlet temperature will substantially reduce emissions, possibly to the point where control devices can be downsized or eliminated. Our findings allow these HAPs reductions to be semiquantitatively estimated.

Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds.

PubMed

Tunçay, Ekin Ö; Demirtaş, T Tolga; Gümüşderelioğlu, Menemşe

2017-03-01

The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds. At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy. Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions. Experiments were carried out with different microwave powers and exposure times, and optimum conditions for the production of B-HAp were determined. B-HAp precipitated from B-SBF by 600W microwave power has 1.15±0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30±0.07% (w/w) carbonate content, 30±4nm rod-like morphology and bone-like amorphous structure. Then, chitosan scaffolds that were prepared by freeze-drying were coated with B-HAp by performing microwave-assisted precipitation in the presence of scaffolds to improve their bioactivities and mechanical properties. The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM). Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer. As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used. The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells. This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications. Copyright © 2017 Elsevier GmbH. All rights reserved.

40 CFR 63.3951 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... reclaimed. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of... coating per liter coating. Wc,i = Mass fraction of organic HAP in coating, i, kg organic HAP per kg coating. For reactive adhesives as defined in § 63.3981, use the mass fraction of organic HAP that is...

40 CFR 63.3951 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2013 CFR

2013-07-01

... reclaimed. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of... coating per liter coating. Wc,i = Mass fraction of organic HAP in coating, i, kg organic HAP per kg coating. For reactive adhesives as defined in § 63.3981, use the mass fraction of organic HAP that is...

40 CFR 63.3951 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... reclaimed. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of... coating per liter coating. Wc,i = Mass fraction of organic HAP in coating, i, kg organic HAP per kg coating. For reactive adhesives as defined in § 63.3981, use the mass fraction of organic HAP that is...

40 CFR 63.4741 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... compliant material option. If the mass fraction of organic HAP of a coating equals zero, determined... fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for...). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures specified...

40 CFR 63.4141 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... the compliant material option. If the mass fraction of organic HAP of a coating equals zero... fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for...). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures specified...

40 CFR 63.4141 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... the compliant material option. If the mass fraction of organic HAP of a coating equals zero... fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for...). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures specified...

40 CFR 63.4741 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... compliant material option. If the mass fraction of organic HAP of a coating equals zero, determined... fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for...). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures specified...

40 CFR 63.4321 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... dyeing or finishing material with a mass fraction of organic HAP that exceeds the applicable emission... fraction of organic HAP for each material. You must determine the mass fraction of organic HAP for each... (appendix A to 40 CFR part 63). You may use Method 311 for determining the mass fraction of organic HAP. Use...

40 CFR 63.4321 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... dyeing or finishing material with a mass fraction of organic HAP that exceeds the applicable emission... fraction of organic HAP for each material. You must determine the mass fraction of organic HAP for each... (appendix A to 40 CFR part 63). You may use Method 311 for determining the mass fraction of organic HAP. Use...

40 CFR 63.4310 - What notifications must I submit?

Code of Federal Regulations, 2013 CFR

2013-07-01

... need to submit copies of any test reports. (i) Mass fraction of organic HAP and mass fraction of solids for one coating or printing formulation including thinning materials, mass fraction of organic HAP for one cleaning material and mass fraction of organic HAP for all of the regulated materials as purchased...

40 CFR 63.4310 - What notifications must I submit?

Code of Federal Regulations, 2012 CFR

2012-07-01

... need to submit copies of any test reports. (i) Mass fraction of organic HAP and mass fraction of solids for one coating or printing formulation including thinning materials, mass fraction of organic HAP for one cleaning material and mass fraction of organic HAP for all of the regulated materials as purchased...

40 CFR 63.4310 - What notifications must I submit?

Code of Federal Regulations, 2014 CFR

2014-07-01

... need to submit copies of any test reports. (i) Mass fraction of organic HAP and mass fraction of solids for one coating or printing formulation including thinning materials, mass fraction of organic HAP for one cleaning material and mass fraction of organic HAP for all of the regulated materials as purchased...

Peat Water Purification by Hydroxyapatite (HAp) Synthesized from Waste Pensi (Corbicula moltkiana) Shells

NASA Astrophysics Data System (ADS)

Fajri Alif, Matlal; Aprillia, Wandha; Arief, Syukri

2018-01-01

Hydroxyapatite (HAP) were synthesized from Pensi (Corbicula moltkiana) sheels by hydrothermal method and used as adsorbent for peat water purification. Batch adsorption experiments were performed to investigate the effects of various factors such as contact time, adsorbent dosage, and pH. The obtained materials were characterized by powder X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). Results showed that HAP calcined at 900°C (HAP900) and 1000°C (HAP1000) have a poorly crystalline shape. HAP900 also contain Tetracalsium Phosphate (TTCP) with a Ca/P molar ratio 2.18, while HAP 1000 contain HAp with a Ca/P molar ratio 1.67. Optimum condition for peat water purification with HAP900 and HAP1000 were both achieved at 1 hours, 1 grams adsorben mass at pH 2. SEM micrographs show that after purification, the surface of HAP were covered by organic compounds from peat water.

40 CFR 63.3531 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2011 CFR

2011-07-01

... be performed with either metric or English units. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of organic HAP for each coating and thinner used during each... thinner, j, kg per liter. Wt,j = Mass fraction of organic HAP in thinner, j, kg organic HAP per kg thinner...

40 CFR 63.4310 - What notifications must I submit?

Code of Federal Regulations, 2011 CFR

2011-07-01

... according to § 63.4321(e)(1) or (2). You do not need to submit copies of any test reports. (i) Mass fraction of organic HAP and mass fraction of solids for one coating or printing formulation including thinning materials, mass fraction of organic HAP for one cleaning material and mass fraction of organic HAP for all...

40 CFR 63.4130 - What records must I keep?

Code of Federal Regulations, 2012 CFR

2012-07-01

... to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning... mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of... period. (e) A record of the mass fraction of organic HAP for each coating, thinner, and cleaning material...

40 CFR 63.4130 - What records must I keep?

Code of Federal Regulations, 2014 CFR

2014-07-01

... to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning... mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of... period. (e) A record of the mass fraction of organic HAP for each coating, thinner, and cleaning material...

40 CFR 63.4130 - What records must I keep?

Code of Federal Regulations, 2013 CFR

2013-07-01

... to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning... mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of... period. (e) A record of the mass fraction of organic HAP for each coating, thinner, and cleaning material...

40 CFR 63.3930 - What records must I keep?

Code of Federal Regulations, 2011 CFR

2011-07-01

... formulation data, or test data used to determine the mass fraction of organic HAP and density for each coating... coating. If you conducted testing to determine mass fraction of organic HAP, density, or volume fraction... rather than a record of the volume used. (e) A record of the mass fraction of organic HAP for each...

40 CFR 63.2465 - What requirements must I meet for process vents that emit hydrogen halide and halogen HAP or HAP...

Code of Federal Regulations, 2010 CFR

2010-07-01

... Pollutants: Miscellaneous Organic Chemical Manufacturing Emission Limits, Work Practice Standards, and... the mass emission rate of HAP metals based on process knowledge, engineering assessment, or test data...

40 CFR 63.4751 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... rate without add-on controls option. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of organic HAP for each coating, thinner, and cleaning material used during each... of coating, i, grams coating per liter coating. Wc,i = Mass fraction of organic HAP in coating, i...

40 CFR 63.4751 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... rate without add-on controls option. (a) Determine the mass fraction of organic HAP for each material. Determine the mass fraction of organic HAP for each coating, thinner, and cleaning material used during each... of coating, i, grams coating per liter coating. Wc,i = Mass fraction of organic HAP in coating, i...

40 CFR 63.3151 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... paragraph (d) of this section. (a) Determine the mass fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for each material used during the compliance period by... 40 CFR part 63). You may use Method 311 for determining the mass fraction of organic HAP. Use the...

In situ remediation and phytotoxicity assessment of lead-contaminated soil by biochar-supported nHAP.

PubMed

Yang, Zhangmei; Fang, Zhanqiang; Tsang, Pokeung Eric; Fang, Jianzhang; Zhao, Dongye

2016-11-01

In this study, a kind of biochar-supported nano-hydroxyapatite (nHAP@BC) material was used in in-situ remediation of lead-contaminated soil. Column experiments were performed to compare the mobility of nHAP@BC and Bare-nHAP. The immobilization, accumulation and toxic effects of Pb in the after-amended soil were assessed by the in vitro toxicity tests and pot experiments. The column experiments showed a significant improvement in the mobility of nHAP@BC. The immobilization rate of Pb in the soil was 74.8% after nHAP@BC remediation. Sequential extraction procedures revealed that the residual fraction of Pb increased by 66.6% after nHAP@BC remediation, which greatly reduced the bioavailability of Pb in the soil. In addition, pot experiments indicated that nHAP@BC could effectively reduce the upward translocation capacity of Pb in a soil-plant system. The concentration of Pb in the aerial part of the cabbage mustard was 0.1 mg/kg, which is lower than the tolerance limit (0.3 mg/kg). nHAP@BC can remediate Pb-contaminated soil effectively, which can restore soil quality for planting. Copyright © 2016 Elsevier Ltd. All rights reserved.

40 CFR 63.4530 - What records must I keep?

Code of Federal Regulations, 2014 CFR

2014-07-01

... formulation data, or test data used to determine the mass fraction of organic HAP and density for each coating, thinner and/or other additive, and cleaning material, and the mass fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic HAP, density, or mass fraction of...

40 CFR 63.4530 - What records must I keep?

Code of Federal Regulations, 2013 CFR

2013-07-01

... formulation data, or test data used to determine the mass fraction of organic HAP and density for each coating, thinner and/or other additive, and cleaning material, and the mass fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic HAP, density, or mass fraction of...

40 CFR 63.4530 - What records must I keep?

Code of Federal Regulations, 2012 CFR

2012-07-01

... formulation data, or test data used to determine the mass fraction of organic HAP and density for each coating, thinner and/or other additive, and cleaning material, and the mass fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic HAP, density, or mass fraction of...

40 CFR 63.3512 - What records must I keep?

Code of Federal Regulations, 2012 CFR

2012-07-01

... used to determine the mass fraction of organic HAP and density for each coating and thinner and the volume fraction of coating solids for each coating. If you conducted testing to determine mass fraction...) A record of the mass fraction of organic HAP for each coating and thinner used during each...

40 CFR 63.3512 - What records must I keep?

Code of Federal Regulations, 2013 CFR

2013-07-01

... used to determine the mass fraction of organic HAP and density for each coating and thinner and the volume fraction of coating solids for each coating. If you conducted testing to determine mass fraction...) A record of the mass fraction of organic HAP for each coating and thinner used during each...

40 CFR 63.4730 - What records must I keep?

Code of Federal Regulations, 2011 CFR

2011-07-01

... to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning... mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of..., and cleaning material used during each compliance period. (e) A record of the mass fraction of organic...

40 CFR 63.3512 - What records must I keep?

Code of Federal Regulations, 2014 CFR

2014-07-01

... used to determine the mass fraction of organic HAP and density for each coating and thinner and the volume fraction of coating solids for each coating. If you conducted testing to determine mass fraction...) A record of the mass fraction of organic HAP for each coating and thinner used during each...

40 CFR 63.4730 - What records must I keep?

Code of Federal Regulations, 2012 CFR

2012-07-01

... used to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning... mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of..., and cleaning material used during each compliance period. (e) A record of the mass fraction of organic...

40 CFR 63.4730 - What records must I keep?

Code of Federal Regulations, 2013 CFR

2013-07-01

... used to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning... mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of..., and cleaning material used during each compliance period. (e) A record of the mass fraction of organic...

40 CFR 63.4730 - What records must I keep?

Code of Federal Regulations, 2014 CFR

2014-07-01

... used to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning... mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of..., and cleaning material used during each compliance period. (e) A record of the mass fraction of organic...

24 CFR 891.560 - HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false HAP contract. 891.560 Section 891... Assistance § 891.560 HAP contract. (a) HAP contract. The housing assistance payments contract sets forth.... (b) HAP contract execution. (1) Upon satisfactory completion of the project, the Borrower and HUD...

40 CFR 63.4312 - What records must I keep?

Code of Federal Regulations, 2014 CFR

2014-07-01

... manufacturer's formulation data or test data used to determine the mass fraction of organic HAP for coating, printing, slashing, dyeing, finishing, thinning, and cleaning materials; and the mass fraction of solids for coating and printing materials. If you conducted testing to determine mass fraction of organic HAP...

40 CFR 63.4312 - What records must I keep?

Code of Federal Regulations, 2012 CFR

2012-07-01

... manufacturer's formulation data or test data used to determine the mass fraction of organic HAP for coating, printing, slashing, dyeing, finishing, thinning, and cleaning materials; and the mass fraction of solids for coating and printing materials. If you conducted testing to determine mass fraction of organic HAP...

40 CFR 63.3521 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2013 CFR

2013-07-01

.... (a) Determine the mass fraction of organic HAP for each material used. You must determine the mass...). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures specified... to be 0.5 percent of the material by mass, you do not have to count it. Express the mass fraction of...

40 CFR 63.3521 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

.... (a) Determine the mass fraction of organic HAP for each material used. You must determine the mass...). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures specified... to be 0.5 percent of the material by mass, you do not have to count it. Express the mass fraction of...

40 CFR 63.3521 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

.... (a) Determine the mass fraction of organic HAP for each material used. You must determine the mass...). You may use Method 311 for determining the mass fraction of organic HAP. Use the procedures specified... to be 0.5 percent of the material by mass, you do not have to count it. Express the mass fraction of...

Huntingtin-associated protein-1 (HAP1) regulates endocytosis and interacts with multiple trafficking-related proteins.

PubMed

Mackenzie, Kimberly D; Lim, Yoon; Duffield, Michael D; Chataway, Timothy; Zhou, Xin-Fu; Keating, Damien J

2017-07-01

Huntingtin-associated protein 1 (HAP1) was initially identified as a binding partner of huntingtin, mutations in which underlie Huntington's disease. Subcellular localization and protein interaction data indicate that HAP1 may be important in vesicle trafficking, cell signalling and receptor internalization. In this study, a proteomics approach was used for the identification of novel HAP1-interacting partners to attempt to shed light on the physiological function of HAP1. Using affinity chromatography with HAP1-GST protein fragments bound to Sepharose columns, this study identified a number of trafficking-related proteins that bind to HAP1. Interestingly, many of the proteins that were identified by mass spectrometry have trafficking-related functions and include the clathrin light chain B and Sec23A, an ER to Golgi trafficking vesicle coat component. Using co-immunoprecipitation and GST-binding assays the association between HAP1 and clathrin light chain B has been validated in vitro. This study also finds that HAP1 co-localizes with clathrin light chain B. In line with a physiological function of the HAP1-clathrin interaction this study detected a dramatic reduction in vesicle retrieval and endocytosis in adrenal chromaffin cells. Furthermore, through examination of transferrin endocytosis in HAP1 -/- cortical neurons, this study has determined that HAP1 regulates neuronal endocytosis. In this study, the interaction between HAP1 and Sec23A was also validated through endogenous co-immunoprecipitation in rat brain homogenate. Through the identification of novel HAP1 binding partners, many of which have putative trafficking roles, this study provides us with new insights into the mechanisms underlying the important physiological function of HAP1 as an intracellular trafficking protein through its protein-protein interactions. Copyright © 2017 Elsevier Inc. All rights reserved.

40 CFR 63.4941 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... material option. (a) Determine the mass fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for each coating, thinner, and cleaning material used during the... 311 (appendix A to 40 CFR part 63). You may use Method 311 for determining the mass fraction of...

40 CFR 63.4941 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2013 CFR

2013-07-01

... material option. (a) Determine the mass fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for each coating, thinner, and cleaning material used during the... 311 (appendix A to 40 CFR part 63). You may use Method 311 for determining the mass fraction of...

40 CFR 63.4941 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... material option. (a) Determine the mass fraction of organic HAP for each material used. You must determine the mass fraction of organic HAP for each coating, thinner, and cleaning material used during the... 311 (appendix A to 40 CFR part 63). You may use Method 311 for determining the mass fraction of...

40 CFR 63.4730 - What records must I keep?

Code of Federal Regulations, 2010 CFR

2010-07-01

... to determine the mass fraction of organic HAP and density for each coating, thinner, and cleaning material and the volume fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic HAP, density, or volume fraction of coating solids, you must keep a copy of...

Mass fractionation processes of transition metal isotopes

NASA Astrophysics Data System (ADS)

Zhu, X. K.; Guo, Y.; Williams, R. J. P.; O'Nions, R. K.; Matthews, A.; Belshaw, N. S.; Canters, G. W.; de Waal, E. C.; Weser, U.; Burgess, B. K.; Salvato, B.

2002-06-01

Recent advances in mass spectrometry make it possible to utilise isotope variations of transition metals to address some important issues in solar system and biological sciences. Realisation of the potential offered by these new isotope systems however requires an adequate understanding of the factors controlling their isotope fractionation. Here we show the results of a broadly based study on copper and iron isotope fractionation during various inorganic and biological processes. These results demonstrate that: (1) naturally occurring inorganic processes can fractionate Fe isotope to a detectable level even at temperature ˜1000°C, which challenges the previous view that Fe isotope variations in natural system are unique biosignatures; (2) multiple-step equilibrium processes at low temperatures may cause large mass fractionation of transition metal isotopes even when the fractionation per single step is small; (3) oxidation-reduction is an importation controlling factor of isotope fractionation of transition metal elements with multiple valences, which opens a wide range of applications of these new isotope systems, ranging from metal-silicate fractionation in the solar system to uptake pathways of these elements in biological systems; (4) organisms incorporate lighter isotopes of transition metals preferentially, and transition metal isotope fractionation occurs stepwise along their pathways within biological systems during their uptake.

40 CFR 63.829 - Recordkeeping requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... mass of all HAP containing materials used and the mass fraction of HAP present in each HAP containing... the criteria of § 63.821(b)(1), the owner or operator shall maintain records of the total mass of each... maintain records of the total mass and organic HAP content of each material applied on product and...

40 CFR 63.829 - Recordkeeping requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... mass of all HAP containing materials used and the mass fraction of HAP present in each HAP containing... the criteria of § 63.821(b)(1), the owner or operator shall maintain records of the total mass of each... maintain records of the total mass and organic HAP content of each material applied on product and...

40 CFR 63.829 - Recordkeeping requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... mass of all HAP containing materials used and the mass fraction of HAP present in each HAP containing... the criteria of § 63.821(b)(1), the owner or operator shall maintain records of the total mass of each... maintain records of the total mass and organic HAP content of each material applied on product and...

40 CFR 63.829 - Recordkeeping requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... mass of all HAP containing materials used and the mass fraction of HAP present in each HAP containing... the criteria of § 63.821(b)(1), the owner or operator shall maintain records of the total mass of each... maintain records of the total mass and organic HAP content of each material applied on product and...

40 CFR 63.4322 - How do I demonstrate continuous compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... which the mass fraction of organic HAP, determined according to the requirements of § 63.4321(e)(1)(iv... which the mass fraction of organic HAP exceeded the applicable emission limit in Table 1 to this subpart...

40 CFR 63.4322 - How do I demonstrate continuous compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... which the mass fraction of organic HAP, determined according to the requirements of § 63.4321(e)(1)(iv... which the mass fraction of organic HAP exceeded the applicable emission limit in Table 1 to this subpart...

40 CFR 63.4322 - How do I demonstrate continuous compliance with the emission limitations?

Code of Federal Regulations, 2011 CFR

2011-07-01

... which the mass fraction of organic HAP, determined according to the requirements of § 63.4321(e)(1)(iv... which the mass fraction of organic HAP exceeded the applicable emission limit in Table 1 to this subpart...

40 CFR 63.4322 - How do I demonstrate continuous compliance with the emission limitations?

Code of Federal Regulations, 2013 CFR

2013-07-01

... which the mass fraction of organic HAP, determined according to the requirements of § 63.4321(e)(1)(iv... which the mass fraction of organic HAP exceeded the applicable emission limit in Table 1 to this subpart...

40 CFR 63.3930 - What records must I keep?

Code of Federal Regulations, 2012 CFR

2012-07-01

... manufacturer's formulation data, or test data used to determine the mass fraction of organic HAP and density for each coating, thinner and/or other additive, and cleaning material, and the volume fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic HAP...

40 CFR 63.3930 - What records must I keep?

Code of Federal Regulations, 2013 CFR

2013-07-01

... manufacturer's formulation data, or test data used to determine the mass fraction of organic HAP and density for each coating, thinner and/or other additive, and cleaning material, and the volume fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic HAP...

40 CFR 63.3930 - What records must I keep?

Code of Federal Regulations, 2014 CFR

2014-07-01

... manufacturer's formulation data, or test data used to determine the mass fraction of organic HAP and density for each coating, thinner and/or other additive, and cleaning material, and the volume fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic HAP...

40 CFR 63.2465 - What requirements must I meet for process vents that emit hydrogen halide and halogen HAP or HAP...

Code of Federal Regulations, 2014 CFR

2014-07-01

... process vents that emit hydrogen halide and halogen HAP or HAP metals? 63.2465 Section 63.2465 Protection... hydrogen halide and halogen HAP or HAP metals? (a) You must meet each emission limit in Table 3 to this...) of this section. (b) If any process vents within a process emit hydrogen halide and halogen HAP, you...

40 CFR 63.2465 - What requirements must I meet for process vents that emit hydrogen halide and halogen HAP or HAP...

Code of Federal Regulations, 2013 CFR

2013-07-01

... process vents that emit hydrogen halide and halogen HAP or HAP metals? 63.2465 Section 63.2465 Protection... hydrogen halide and halogen HAP or HAP metals? (a) You must meet each emission limit in Table 3 to this...) of this section. (b) If any process vents within a process emit hydrogen halide and halogen HAP, you...

40 CFR 63.2465 - What requirements must I meet for process vents that emit hydrogen halide and halogen HAP or HAP...

Code of Federal Regulations, 2012 CFR

2012-07-01

... process vents that emit hydrogen halide and halogen HAP or HAP metals? 63.2465 Section 63.2465 Protection... hydrogen halide and halogen HAP or HAP metals? (a) You must meet each emission limit in Table 3 to this...) of this section. (b) If any process vents within a process emit hydrogen halide and halogen HAP, you...

24 CFR 891.580 - HAP contract administration.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false HAP contract administration. 891... Handicapped-Section 8 Assistance § 891.580 HAP contract administration. HUD is responsible for the administration of the HAP contract. ...

40 CFR 63.3512 - What records must I keep?

Code of Federal Regulations, 2011 CFR

2011-07-01

... for each coating. If you conducted testing to determine mass fraction of organic HAP, density, or... and thinner used during each compliance period. (e) A record of the mass fraction of organic HAP for... suppliers or manufacturers, such as manufacturer's formulation data, or test data used to determine the mass...

24 CFR 983.202 - Purpose of HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Purpose of HAP contract. 983.202... DEVELOPMENT PROJECT-BASED VOUCHER (PBV) PROGRAM Housing Assistance Payments Contract § 983.202 Purpose of HAP contract. (a) Requirement. The PHA must enter into a HAP contract with the owner. The HAP contract must be...

40 CFR 63.4510 - What notifications must I submit?

Code of Federal Regulations, 2013 CFR

2013-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner and/or other additive, and for one cleaning material. (ii) Mass fraction of coating solids... required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste materials...

40 CFR 63.824 - Standards: Publication rotogravure printing.

Code of Federal Regulations, 2012 CFR

2012-07-01

....000 For the purposes of this calculation, the mass fraction of organic HAP present in the recovered volatile matter is assumed to be equal to the mass fraction of organic HAP present in the volatile matter... section: (i) Perform a liquid-liquid material balance for each month as follows: (A) Measure the mass of...

40 CFR 63.4510 - What notifications must I submit?

Code of Federal Regulations, 2011 CFR

2011-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner and/or other additive, and for one cleaning material. (ii) Mass fraction of coating solids... required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste materials...

40 CFR 63.824 - Standards: Publication rotogravure printing.

Code of Federal Regulations, 2013 CFR

2013-07-01

....000 For the purposes of this calculation, the mass fraction of organic HAP present in the recovered volatile matter is assumed to be equal to the mass fraction of organic HAP present in the volatile matter... section: (i) Perform a liquid-liquid material balance for each month as follows: (A) Measure the mass of...

40 CFR 63.4510 - What notifications must I submit?

Code of Federal Regulations, 2012 CFR

2012-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner and/or other additive, and for one cleaning material. (ii) Mass fraction of coating solids... required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste materials...

40 CFR 63.4510 - What notifications must I submit?

Code of Federal Regulations, 2014 CFR

2014-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner and/or other additive, and for one cleaning material. (ii) Mass fraction of coating solids... required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste materials...

24 CFR 891.565 - Term of HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Term of HAP contract. 891.565... 8 Assistance § 891.565 Term of HAP contract. The term of the HAP contract for assisted units shall be 20 years. If the project is completed in stages, the term of the HAP contract for assisted units...

24 CFR 983.203 - HAP contract information.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false HAP contract information. 983.203... DEVELOPMENT PROJECT-BASED VOUCHER (PBV) PROGRAM Housing Assistance Payments Contract § 983.203 HAP contract information. The HAP contract must specify: (a) The total number of contract units by number of bedrooms; (b...

40 CFR 63.5335 - How do I determine the actual HAP loss?

Code of Federal Regulations, 2010 CFR

2010-07-01

... operation type. (2) Chemical Inventory Mass Balance. Determine the actual monthly HAP loss from your... operation. (2) For facilities using add-on emission control devices, the finish inventory log and the... National Emission Standards for Hazardous Air Pollutants for Leather Finishing Operations Compliance...

Intersystem Interference Reduction for Overlaid HAPS-Terrestrial CDMA System

NASA Astrophysics Data System (ADS)

Huang, Jeng-Ji; Wang, Wei-Ting; Li, Mingfu; Shiung, David; Ferng, Huei-Wen

In this letter, we propose that directional antennas, combined with power management, be incorporated to reduce intersystem interference in a shared band overlaid high altitude platform station (HAPS)-terrestrial code division multiple access (CDMA) system. To eliminate the HAPS to terrestrial interference, the HAPS is accessed only via directional antennas under the proposed scheme. By doing so, the uplink power to the HAPS can accordingly be increased, so that the terrestrial to HAPS interference is also effectively suppressed.

40 CFR Table 6 to Subpart Vvvv of... - Default Organic HAP Contents of Petroleum Solvent Groups

Code of Federal Regulations, 2011 CFR

2011-07-01

... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for Boat... content, percent by mass Typical organic HAP, percent by mass Aliphatic (Mineral Spirits 135, Mineral...

Effect of stearic acid modified HAp nanoparticles in different solvents on the properties of Pickering emulsions and HAp/PLLA composites.

PubMed

Zhang, Ming; Wang, Ai-Juan; Li, Jun-Ming; Song, Na

2017-10-01

Stearic acid (Sa) was used to modify the surface properties of hydroxyapatite (HAp) in different solvents (water, ethanol or dichloromethane(CH 2 Cl 2 )). Effect of different solvents on the properties of HAp particles (activation ratio, grafting ratio, chemical properties), emulsion properties (emulsion stability, emulsion type, droplet morphology) as well as the cured materials (morphology, average pore size) were studied. FT-IR and XPS results confirmed the interaction occurred between stearic acid and HAp particles. Stable O/W and W/O type Pickering emulsions were prepared using unmodified and Sa modified HAp nanoparticles respectively, which indicated a catastrophic inversion of the Pickering emulsion happened possibly because of the enhanced hydrophobicity of HAp particles after surface modification. Porous materials with different structures and pore sizes were obtained using Pickering emulsion as the template via in situ evaporation solvent method. The results indicated the microstructures of cured samples are different form each other when HAp was surface modified in different solvents. HAp particles fabricated using ethanol as solvent has higher activation ratio and grafting ratio. Pickering emulsion with higher stability and cured porous materials with uniform morphology were obtained compared with samples prepared using water and CH 2 Cl 2 as solvents. In conclusion, surface modification of HAp in different solvents played a very important role for its stabilized Pickering emulsion as well as the microstructure of cured samples. It is better to use ethanol as the solvent for Sa modified HAp particles, which could increase the stability of Pickering emulsion and obtain cured samples with uniform pore size. Copyright © 2017 Elsevier B.V. All rights reserved.

40 CFR 63.4910 - What notifications must I submit?

Code of Federal Regulations, 2011 CFR

2011-07-01

... need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner, and for one cleaning material. (ii) Volume fraction of coating solids for one coating. (iii... mass of organic HAP contained in the waste materials for which you are claiming an allowance in...

40 CFR 63.4710 - What notifications must I submit?

Code of Federal Regulations, 2014 CFR

2014-07-01

...) Mass fraction of organic HAP for one coating, for one thinner, and for one cleaning material. (ii) Volume fraction of coating solids for one coating. (iii) Density for one coating, one thinner, and one... is required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste...

40 CFR 63.4910 - What notifications must I submit?

Code of Federal Regulations, 2014 CFR

2014-07-01

... need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner, and for one cleaning material. (ii) Volume fraction of coating solids for one coating. (iii... mass of organic HAP contained in the waste materials for which you are claiming an allowance in...

40 CFR 63.4710 - What notifications must I submit?

Code of Federal Regulations, 2012 CFR

2012-07-01

...) Mass fraction of organic HAP for one coating, for one thinner, and for one cleaning material. (ii) Volume fraction of coating solids for one coating. (iii) Density for one coating, one thinner, and one... is required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste...

40 CFR 63.4910 - What notifications must I submit?

Code of Federal Regulations, 2013 CFR

2013-07-01

... need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner, and for one cleaning material. (ii) Volume fraction of coating solids for one coating. (iii... mass of organic HAP contained in the waste materials for which you are claiming an allowance in...

40 CFR 63.4710 - What notifications must I submit?

Code of Federal Regulations, 2013 CFR

2013-07-01

...) Mass fraction of organic HAP for one coating, for one thinner, and for one cleaning material. (ii) Volume fraction of coating solids for one coating. (iii) Density for one coating, one thinner, and one... is required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste...

24 CFR 891.590 - Notice upon HAP contract expiration.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Notice upon HAP contract expiration... Handicapped-Section 8 Assistance § 891.590 Notice upon HAP contract expiration. (a) Notice required. The HAP contract will provide that the Borrower will, at least one year before the end of the HAP contract term...

24 CFR 983.204 - When HAP contract is executed.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false When HAP contract is executed. 983... When HAP contract is executed. (a) PHA inspection of housing. (1) Before execution of the HAP contract... into a HAP contract for any contract unit until the PHA has determined that the unit complies with the...

Acoustic emission detection for mass fractions of materials based on wavelet packet technology.

PubMed

Wang, Xianghong; Xiang, Jianjun; Hu, Hongwei; Xie, Wei; Li, Xiongbing

2015-07-01

Materials are often damaged during the process of detecting mass fractions by traditional methods. Acoustic emission (AE) technology combined with wavelet packet analysis is used to evaluate the mass fractions of microcrystalline graphite/polyvinyl alcohol (PVA) composites in this study. Attenuation characteristics of AE signals across the composites with different mass fractions are investigated. The AE signals are decomposed by wavelet packet technology to obtain the relationships between the energy and amplitude attenuation coefficients of feature wavelet packets and mass fractions as well. Furthermore, the relationship is validated by a sample. The larger proportion of microcrystalline graphite will correspond to the higher attenuation of energy and amplitude. The attenuation characteristics of feature wavelet packets with the frequency range from 125 kHz to 171.85 kHz are more suitable for the detection of mass fractions than those of the original AE signals. The error of the mass fraction of microcrystalline graphite calculated by the feature wavelet packet (1.8%) is lower than that of the original signal (3.9%). Therefore, AE detection base on wavelet packet analysis is an ideal NDT method for evaluate mass fractions of composite materials. Copyright © 2015 Elsevier B.V. All rights reserved.

40 CFR 63.4710 - What notifications must I submit?

Code of Federal Regulations, 2011 CFR

2011-07-01

...). You do not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one thinner, and for one cleaning material. (ii) Volume fraction of coating solids for one coating... and the mass of organic HAP contained in the waste materials for which you are claiming an allowance...

40 CFR 63.3510 - What notifications must I submit?

Code of Federal Regulations, 2014 CFR

2014-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating and for one thinner. (ii) Volume fraction of coating solids for one coating. (iii) Density for one coating... density is required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste...

40 CFR 63.3510 - What notifications must I submit?

Code of Federal Regulations, 2013 CFR

2013-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating and for one thinner. (ii) Volume fraction of coating solids for one coating. (iii) Density for one coating... density is required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste...

40 CFR 63.3510 - What notifications must I submit?

Code of Federal Regulations, 2011 CFR

2011-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating and for one thinner. (ii) Volume fraction of coating solids for one coating. (iii) Density for one coating... density is required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste...

40 CFR 63.3510 - What notifications must I submit?

Code of Federal Regulations, 2012 CFR

2012-07-01

... not need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating and for one thinner. (ii) Volume fraction of coating solids for one coating. (iii) Density for one coating... density is required. (iv) The amount of waste materials and the mass of organic HAP contained in the waste...

40 CFR 63.3151 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2010 CFR

2010-07-01

... matter and use that value as a substitute for mass fraction of organic HAP. (3) Alternative method. You may use an alternative test method for determining the mass fraction of organic HAP once the Administrator has approved it. You must follow the procedure in § 63.7(f) to submit an alternative test method...

Antenna Beam Pattern Characteristics of HAPS User Terminal

NASA Astrophysics Data System (ADS)

Ku, Bon-Jun; Oh, Dae Sub; Kim, Nam; Ahn, Do-Seob

High Altitude Platform Stations (HAPS) are recently considered as a green infrastructure to provide high speed multimedia services. The critical issue of HAPS is frequency sharing with satellite systems. Regulating antenna beam pattern using adaptive antenna schemes is one of means to facilitate the sharing with a space receiver for fixed satellite services on the uplink of a HAPS system operating in U bands. In this letter, we investigate antenna beam pattern characteristics of HAPS user terminals with various values of scan angles of main beam, null position angles, and null width.

40 CFR 63.2465 - What requirements must I meet for process vents that emit hydrogen halide and halogen HAP or HAP...

Code of Federal Regulations, 2011 CFR

2011-07-01

... process vents that emit hydrogen halide and halogen HAP or HAP metals? 63.2465 Section 63.2465 Protection... Compliance Requirements § 63.2465 What requirements must I meet for process vents that emit hydrogen halide... section. (b) If any process vents within a process emit hydrogen halide and halogen HAP, you must...

Hap1 and GABA: thinking about food intake.

PubMed

Woods, Stephen C; Seeley, Randy J

2006-06-01

GABA stimulation of hypothalamic GABAA receptors increases food intake and body weight. Huntingtin-associated protein-1 (Hap1), is highly expressed in the hypothalamus and increases activity at GABAA receptors; mice lacking Hap1 are hypophagic. A recent paper (Sheng et al.,2006) further explores the role of Hap1 in the control of food intake.

HapMap filter 1.0: a tool to preprocess the HapMap genotypic data for association studies.

PubMed

Zhang, Wei; Duan, Shiwei; Dolan, M Eileen

2008-05-13

The International HapMap Project provides a resource of genotypic data on single nucleotide polymorphisms (SNPs), which can be used in various association studies to identify the genetic determinants for phenotypic variations. Prior to the association studies, the HapMap dataset should be preprocessed in order to reduce the computation time and control the multiple testing problem. The less informative SNPs including those with very low genotyping rate and SNPs with rare minor allele frequencies to some extent in one or more population are removed. Some research designs only use SNPs in a subset of HapMap cell lines. Although the HapMap website and other association software packages have provided some basic tools for optimizing these datasets, a fast and user-friendly program to generate the output for filtered genotypic data would be beneficial for association studies. Here, we present a flexible, straight-forward bioinformatics program that can be useful in preparing the HapMap genotypic data for association studies by specifying cell lines and two common filtering criteria: minor allele frequencies and genotyping rate. The software was developed for Microsoft Windows and written in C++. The Windows executable and source code in Microsoft Visual C++ are available at Google Code (http://hapmap-filter-v1.googlecode.com/) or upon request. Their distribution is subject to GNU General Public License v3.

40 CFR Table 3 to Subpart Ffff of... - Emission Limits for Hydrogen Halide and Halogen HAP Emissions or HAP Metals Emissions From...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Emission Limits for Hydrogen Halide and... to Subpart FFFF of Part 63—Emission Limits for Hydrogen Halide and Halogen HAP Emissions or HAP... following table that applies to your process vents that contain hydrogen halide and halogen HAP emissions or...

40 CFR Table 3 to Subpart Ffff of... - Emission Limits for Hydrogen Halide and Halogen HAP Emissions or HAP Metals Emissions From...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Emission Limits for Hydrogen Halide and... to Subpart FFFF of Part 63—Emission Limits for Hydrogen Halide and Halogen HAP Emissions or HAP... following table that applies to your process vents that contain hydrogen halide and halogen HAP emissions or...

24 CFR 982.603 - SRO: Lease and HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false SRO: Lease and HAP contract. 982... Types Single Room Occupancy (sro) § 982.603 SRO: Lease and HAP contract. For SRO housing, there is a separate lease and HAP contract for each assisted person. ...

Isotope mass fractionation during evaporation of Mg2SiO4

NASA Technical Reports Server (NTRS)

Davis, Andrew M.; Clayton, Robert N.; Mayeda, Toshiko K.; Hashimoto, Akihiko

1990-01-01

Synthetic forsterite (Mg2SiO4) was partially evaporated in vacuum for various durations and at different temperatures. The residual charges obtained when molten Mg2SiO4 was evaporated to 12 percent of its initial mass were enriched in heavy isotopes by about 20, 30, and 15 per mil/amu for O, Mg, and Si, respectively, whereas solid forsterite evaporated to a similar residual mass fraction showed negligible fractionations. These results imply that calcium and aluminum-rich refractory inclusions in carbonaceous chondrites must have been at least partially molten in the primordial solar nebula if the observed large mass fractionation effects were caused by evaporation processes in the nebula.

24 CFR 982.455 - Automatic termination of HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Automatic termination of HAP contract. 982.455 Section 982.455 Housing and Urban Development Regulations Relating to Housing and Urban... Payments Contract and Owner Responsibility § 982.455 Automatic termination of HAP contract. The HAP...

Structural Determinants of Autoproteolysis of the Haemophilus influenzae Hap Autotransporter▿

PubMed Central

Kenjale, Roma; Meng, Guoyu; Fink, Doran L.; Juehne, Twyla; Ohashi, Tomoo; Erickson, Harold P.; Waksman, Gabriel; St. Geme, Joseph W.

2009-01-01

Haemophilus influenzae is a gram-negative bacterium that initiates infection by colonizing the upper respiratory tract. The H. influenzae Hap autotransporter protein mediates adherence, invasion, and microcolony formation in assays with respiratory epithelial cells and presumably facilitates colonization. The serine protease activity of Hap is associated with autoproteolytic cleavage and extracellular release of the HapS passenger domain, leaving the Hapβ C-terminal domain embedded in the outer membrane. Cleavage occurs most efficiently at the LN1036-37 peptide bond and to a lesser extent at three other sites. In this study, we utilized site-directed mutagenesis, homology modeling, and assays with a peptide library to characterize the structural determinants of Hap proteolytic activity and cleavage specificity. In addition, we used homology modeling to predict the S1, S2, and S4 subsite residues of the Hap substrate groove. Our results indicate that the P1 and P2 positions at the Hap cleavage sites are critical for cleavage, with leucine preferred over larger hydrophobic residues or other amino acids in these positions. The substrate groove is formed by L263 and N274 at the S1 subsite, R264 at the S2 subsite, and E265 at the S4 subsite. This information may facilitate design of approaches to block Hap activity and interfere with H. influenzae colonization. PMID:19687208

Recombinant C-terminal 311 amino acids of HapS adhesin as a vaccine candidate for nontypeable Haemophilus influenzae: A study on immunoreactivity in Balb/C mouse.

PubMed

Tabatabaee Bafroee, Akram Sadat; Siadat, Seyed Davar; Mousavi, Seyed Fazlollah; Aghasadeghi, Mohammad Reza; Khorsand, Hashem; Nejati, Mehdi; Sadat, Seyed Mehdi; Mahdavi, Mehdi

2016-09-01

Hap, an auto-transporter protein, is an antigenically conserved adhesion protein which is present on both typeable and nontypeable Haemophilus influenzae. This protein has central role in bacterial attachment to respiratory tract epithelial cells. A 1000bp C-terminal fragment of Hap passenger domain (HapS) from nontypeable Haemophilus influenzae was cloned into a prokaryotic expression vector, pET-24a. BALB/c mice were immunized subcutaneously with purified rC-HapS. Serum IgG responses to purified rC-HapS, serum IgG subclasses were determined by ELISA and functional activity of antibodies was examined by Serum Bactericidal Assay. The output of rC-HapS was approximately 62% of the total bacterial proteins. Serum IgG responses were significantly increased in immunized group with rC-HapS mixed with Freund's adjuvant in comparison with control groups. Analysis of the serum IgG subclasses showed that the IgG1 subclass was predominant after subcutaneous immunization in BALB/c mice (IgG2a/IgG1 < 1). The sera from rC-HapS immunized animals were strongly bactericidal against nontypeable Haemophilus influenzae. These results suggest that rC-HapS may be a potential vaccine candidate for nontypeable Haemophilus influenzae. Copyright © 2016 Elsevier Ltd. All rights reserved.

24 CFR 891.595 - HAP contract extension or renewal.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false HAP contract extension or renewal... Handicapped-Section 8 Assistance § 891.595 HAP contract extension or renewal. Upon expiration of the term of the HAP contract, HUD and the Borrower may agree (subject to available funds) to extend the term of...

Structural determinants of the interaction between the Haemophilus influenzae Hap autotransporter and fibronectin.

PubMed

Spahich, Nicole A; Kenjale, Roma; McCann, Jessica; Meng, Guoyu; Ohashi, Tomoo; Erickson, Harold P; St Geme, Joseph W

2014-06-01

Haemophilus influenzae is a Gram-negative cocco-bacillus that initiates infection by colonizing the upper respiratory tract. Hap is an H. influenzae serine protease autotransporter protein that mediates adherence, invasion and microcolony formation in assays with human epithelial cells and is presumed to facilitate the process of colonization. Additionally, Hap mediates adherence to fibronectin, laminin and collagen IV, extracellular matrix (ECM) proteins that are present in the respiratory tract and are probably important targets for H. influenzae colonization. The region of Hap responsible for adherence to ECM proteins has been localized to the C-terminal 511 aa of the Hap passenger domain (HapS). In this study, we characterized the structural determinants of the interaction between HapS and fibronectin. Using defined fibronectin fragments, we established that Hap interacts with the fibronectin repeat fragment called FNIII(1-2). Using site-directed mutagenesis, we found a series of motifs in the C-terminal region of HapS that contribute to the interaction with fibronectin. Most of these motifs are located on the F1 and F3 faces of the HapS structure, suggesting that the F1 and F3 faces may be responsible for the HapS-fibronectin interaction. © 2014 The Authors.

Structural determinants of the interaction between the Haemophilus influenzae Hap autotransporter and fibronectin

PubMed Central

Spahich, Nicole A.; Kenjale, Roma; McCann, Jessica; Meng, Guoyu; Ohashi, Tomoo; Erickson, Harold P.

2014-01-01

Haemophilus influenzae is a Gram-negative cocco-bacillus that initiates infection by colonizing the upper respiratory tract. Hap is an H. influenzae serine protease autotransporter protein that mediates adherence, invasion and microcolony formation in assays with human epithelial cells and is presumed to facilitate the process of colonization. Additionally, Hap mediates adherence to fibronectin, laminin and collagen IV, extracellular matrix (ECM) proteins that are present in the respiratory tract and are probably important targets for H. influenzae colonization. The region of Hap responsible for adherence to ECM proteins has been localized to the C-terminal 511 aa of the Hap passenger domain (HapS). In this study, we characterized the structural determinants of the interaction between HapS and fibronectin. Using defined fibronectin fragments, we established that Hap interacts with the fibronectin repeat fragment called FNIII(1–2). Using site-directed mutagenesis, we found a series of motifs in the C-terminal region of HapS that contribute to the interaction with fibronectin. Most of these motifs are located on the F1 and F3 faces of the HapS structure, suggesting that the F1 and F3 faces may be responsible for the HapS–fibronectin interaction. PMID:24687948

Household air pollution (HAP), microenvironment and child health: Strategies for mitigating HAP exposure in urban Rwanda

NASA Astrophysics Data System (ADS)

Das, Ipsita; Pedit, Joseph; Handa, Sudhanshu; Jagger, Pamela

2018-04-01

Exposure to household air pollution (HAP) from cooking and heating with solid fuels is a major risk factor for morbidity and mortality in sub-Saharan Africa. Children under five are particularly at risk for acute lower respiratory infection. We use baseline data from a randomized controlled trial evaluating a household energy intervention in Gisenyi, Rwanda to investigate the role of the microenvironment as a determinant of children’s HAP-related health symptoms. Our sample includes 529 households, with 694 children under five. We examine the association between likelihood of HAP-related health symptom prevalence and characteristics of the microenvironment including: dwelling and cooking area structure; distance to nearest road; and tree cover. We find that children residing in groups of enclosed dwellings, in households that cook indoors, and in households proximate to tree cover, are significantly more likely to experience symptoms of respiratory infection, illness with cough and difficulty breathing. On the other hand, children in households with cemented floors and ventilation holes in the cooking area, are significantly less likely to experience the same symptoms. Our findings suggest that in addition to promoting increased access to clean cooking technologies, there are important infrastructure and microenvironment-related interventions that mitigate HAP exposure.

Household air pollution (HAP), microenvironment and child health: Strategies for mitigating HAP exposure in urban Rwanda

PubMed Central

Das, Ipsita; Pedit, Joseph; Handa, Sudhanshu; Jagger, Pamela

2018-01-01

Exposure to household air pollution (HAP) from cooking and heating with solid fuels is major risk factor for morbidity and mortality in sub-Saharan Africa. Children under five are particularly at risk for acute lower respiratory infection. We use baseline data from randomized controlled trial evaluating a household energy intervention in Gisenyi, Rwanda to investigate the role of the microenvironment as a determinant of children’s HAP-related health symptoms. Our sample includes 529 households, with 694 children under five. We examine the association between likelihood of HAP-related health symptom prevalence and characteristics of the microenvironment including: dwelling and cooking area structure; distance to nearest road; and tree cover. We find that children residing in groups of enclosed dwellings, in households that cook indoors, and in households proximate to tree cover, are significantly more likely to experience symptoms of respiratory infection, illness with cough and difficulty breathing. On the other hand, children in households with cemented floors and ventilation holes in the cooking area, are significantly less likely to experience the same symptoms. Our findings suggest that in addition to promoting increased access to clean cooking technologies, there are important infrastructure and micro-environment related interventions that mitigate HAP exposure. PMID:29682002

Hybrid Modeling Approach to Estimate Exposures of Hazardous Air Pollutants (HAPs) for the National Air Toxics Assessment (NATA).

PubMed

Scheffe, Richard D; Strum, Madeleine; Phillips, Sharon B; Thurman, James; Eyth, Alison; Fudge, Steve; Morris, Mark; Palma, Ted; Cook, Richard

2016-11-15

A hybrid air quality model has been developed and applied to estimate annual concentrations of 40 hazardous air pollutants (HAPs) across the continental United States (CONUS) to support the 2011 calendar year National Air Toxics Assessment (NATA). By combining a chemical transport model (CTM) with a Gaussian dispersion model, both reactive and nonreactive HAPs are accommodated across local to regional spatial scales, through a multiplicative technique designed to improve mass conservation relative to previous additive methods. The broad scope of multiple pollutants capturing regional to local spatial scale patterns across a vast spatial domain is precedent setting within the air toxics community. The hybrid design exhibits improved performance relative to the stand alone CTM and dispersion model. However, model performance varies widely across pollutant categories and quantifiably definitive performance assessments are hampered by a limited observation base and challenged by the multiple physical and chemical attributes of HAPs. Formaldehyde and acetaldehyde are the dominant HAP concentration and cancer risk drivers, characterized by strong regional signals associated with naturally emitted carbonyl precursors enhanced in urban transport corridors with strong mobile source sector emissions. The multiple pollutant emission characteristics of combustion dominated source sectors creates largely similar concentration patterns across the majority of HAPs. However, reactive carbonyls exhibit significantly less spatial variability relative to nonreactive HAPs across the CONUS.

The dense gas mass fraction of molecular clouds in the Milky Way

DOE Office of Scientific and Technical Information (OSTI.GOV)

Battisti, Andrew J.; Heyer, Mark H., E-mail: abattist@astro.umass.edu, E-mail: heyer@astro.umass.edu

2014-01-10

The mass fraction of dense gas within giant molecular clouds (GMCs) of the Milky Way is investigated using {sup 13}CO data from the Five College Radio Astronomy Observatory Galactic Plane Surveys and the Bolocam Galactic Plane Survey (BGPS) of 1.1 mm dust continuum emission. A sample of 860 compact dust sources are selected from the BGPS catalog and kinematically linked to 344 clouds of extended (>3') {sup 13}CO J = 1-0 emission. Gas masses are tabulated for the full dust source and subregions within the dust sources with mass surface densities greater than 200 M {sub ☉} pc{sup –2}, whichmore » are assumed to be regions of enhanced volume density. Masses of the parent GMCs are calculated assuming optically thin {sup 13}CO J = 1-0 emission and local thermodynamic equilibrium conditions. The mean fractional mass of dust sources to host GMC mass is 0.11{sub −0.06}{sup +0.12}. The high column density subregions comprise 0.07{sub −0.05}{sup +0.13} of the mass of the cloud. Owing to our assumptions, these values are upper limits to the true mass fractions. The fractional mass of dense gas is independent of GMC mass and gas surface density. The low dense gas mass fraction suggests that the formation of dense structures within GMCs is the primary bottleneck for star formation. The distribution of velocity differences between the dense gas and the low density material along the line of sight is also examined. We find a strong, centrally peaked distribution centered on zero velocity displacement. This distribution of velocity differences is modeled with radially converging flows toward the dense gas position that are randomly oriented with respect to the observed line of sight. These models constrain the infall velocities to be 2-4 km s{sup –1} for various flow configurations.« less

24 CFR 982.607 - Congregate housing: Lease and HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Congregate housing: Lease and HAP... Types Congregate Housing § 982.607 Congregate housing: Lease and HAP contract. For congregate housing, there is a separate lease and HAP contract for each assisted family. ...

The Haemophilus influenzae Hap Autotransporter Binds to Fibronectin, Laminin, and Collagen IV

PubMed Central

Fink, Doran L.; Green, Bruce A.; St. Geme III, Joseph W.

2002-01-01

Nontypeable Haemophilus influenzae (NTHI) initiates infection by colonizing the upper respiratory tract mucosa. NTHI disease frequently occurs in the context of respiratory tract inflammation, where organisms encounter damaged epithelium and exposed basement membrane. In this study, we examined interactions between the H. influenzae Hap adhesin and selected extracellular matrix proteins. Hap is an autotransporter protein that undergoes autoproteolytic cleavage, with release of the adhesive passenger domain, Haps, from the bacterial cell surface. We found that Hap promotes bacterial adherence to purified fibronectin, laminin, and collagen IV and that Hap-mediated adherence is enhanced by inhibition of autoproteolysis. Adherence is inhibited by pretreatment of bacteria with a polyclonal antiserum recognizing Haps. Purified Haps binds with high affinity to fibronectin, laminin, and collagen IV but not to collagen II. Binding of Haps to fibronectin involves interaction with the 45-kDa gelatin-binding domain but not the 30-kDa heparin-binding domain of fibronectin. Taken together, these observations suggest that interactions between Hap and extracellular matrix proteins may play an important role in NTHI colonization of the respiratory tract. PMID:12183535

DARK MATTER MASS FRACTION IN LENS GALAXIES: NEW ESTIMATES FROM MICROLENSING

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiménez-Vicente, J.; Mediavilla, E.; Kochanek, C. S.

2015-02-01

We present a joint estimate of the stellar/dark matter mass fraction in lens galaxies and the average size of the accretion disk of lensed quasars based on microlensing measurements of 27 quasar image pairs seen through 19 lens galaxies. The Bayesian estimate for the fraction of the surface mass density in the form of stars is α = 0.21 ± 0.14 near the Einstein radius of the lenses (∼1-2 effective radii). The estimate for the average accretion disk size is R{sub 1/2}=7.9{sub −2.6}{sup +3.8}√(M/0.3 M{sub ⊙}) light days. The fraction of mass in stars at these radii is significantly largermore » than previous estimates from microlensing studies assuming quasars were point-like. The corresponding local dark matter fraction of 79% is in good agreement with other estimates based on strong lensing or kinematics. The size of the accretion disk inferred in the present study is slightly larger than previous estimates.« less

24 CFR 982.616 - Shared housing: Lease and HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Shared housing: Lease and HAP... Types Shared Housing § 982.616 Shared housing: Lease and HAP contract. For assistance in a shared housing unit, there is a separate HAP contract and lease for each assisted family. ...

24 CFR 982.454 - Termination of HAP contract: Insufficient funding.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Termination of HAP contract... Assistance Payments Contract and Owner Responsibility § 982.454 Termination of HAP contract: Insufficient funding. The PHA may terminate the HAP contract if the PHA determines, in accordance with HUD requirements...

Galaxy and Mass Assembly (GAMA): the red fraction and radial distribution of satellite galaxies

NASA Astrophysics Data System (ADS)

Prescott, Matthew; Baldry, I. K.; James, P. A.; Bamford, S. P.; Bland-Hawthorn, J.; Brough, S.; Brown, M. J. I.; Cameron, E.; Conselice, C. J.; Croom, S. M.; Driver, S. P.; Frenk, C. S.; Gunawardhana, M.; Hill, D. T.; Hopkins, A. M.; Jones, D. H.; Kelvin, L. S.; Kuijken, K.; Liske, J.; Loveday, J.; Nichol, R. C.; Norberg, P.; Parkinson, H. R.; Peacock, J. A.; Phillipps, S.; Pimbblet, K. A.; Popescu, C. C.; Robotham, A. S. G.; Sharp, R. G.; Sutherland, W. J.; Taylor, E. N.; Tuffs, R. J.; van Kampen, E.; Wijesinghe, D.

2011-10-01

We investigate the properties of satellite galaxies that surround isolated hosts within the redshift range 0.01 < z < 0.15, using data taken as part of the Galaxy And Mass Assembly survey. Making use of isolation and satellite criteria that take into account stellar mass estimates, we find 3514 isolated galaxies of which 1426 host a total of 2998 satellites. Separating the red and blue populations of satellites and hosts, using colour-mass diagrams, we investigate the radial distribution of satellite galaxies and determine how the red fraction of satellites varies as a function of satellite mass, host mass and the projected distance from their host. Comparing the red fraction of satellites to a control sample of small neighbours at greater projected radii, we show that the increase in red fraction is primarily a function of host mass. The satellite red fraction is about 0.2 higher than the control sample for hosts with ?, while the red fractions show no difference for hosts with ?. For the satellites of more massive hosts, the red fraction also increases as a function of decreasing projected distance. Our results suggest that the likely main mechanism for the quenching of star formation in satellites hosted by isolated galaxies is strangulation.

40 CFR Table 4 to Subpart Oooo of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2014 CFR

2014-07-01

... fraction values in the following table for solvent blends for which you do not have test data or... spirits 64742-89-6 0.15 Toluene. 14. Low aromatic white spirit 64742-82-1 0 None. 15. Mineral spirits...

40 CFR Table 3 to Subpart IIIi of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2014 CFR

2014-07-01

... fraction values in the following table for solvent blends for which you do not have test data or... spirits 64742-89-6 0.15 Toluene. 14. Low aromatic white spirit 64742-82-1 0 None. 15. Mineral spirits...

40 CFR Table 4 to Subpart Oooo of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2012 CFR

2012-07-01

... fraction values in the following table for solvent blends for which you do not have test data or... spirits 64742-89-6 0.15 Toluene. 14. Low aromatic white spirit 64742-82-1 0 None. 15. Mineral spirits...

40 CFR Table 3 to Subpart IIIi of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2012 CFR

2012-07-01

... fraction values in the following table for solvent blends for which you do not have test data or... spirits 64742-89-6 0.15 Toluene. 14. Low aromatic white spirit 64742-82-1 0 None. 15. Mineral spirits...

Mass dependence of calcium isotope fractionations in crown-ether resin chromatography.

PubMed

Fujii, Yasuhiko; Nomura, Masao; Kaneshiki, Tositaka; Sakuma, Yoichi; Suzuki, Tatsuya; Umehara, Saori; Kishimoto, Tadahumi

2010-06-01

Benzo 18-crown-6-ether resin was synthesised by the phenol condensation polymerisation process in porous silica beads, of which particle diameter was ca 60micro Calcium adsorption chromatography was performed with the synthesised resin packed in a glass column. The effluent was sampled in fractions, and the isotopic abundance ratios of (42)Ca, (43)Ca, (44)Ca, and (48)Ca against (40)Ca were measured by a thermo-ionisation mass spectrometer. The enrichment of heavier calcium isotopes was observed at the front boundary of calcium adsorption chromatogram. The mass dependence of mutual separation of calcium isotopes was analysed by using the three-isotope-plots method. The slopes of three-isotope-plots indicate the relative values of mutual separation coefficients for concerned isotopic pairs. The results have shown the normal mass dependence; isotope fractionation is proportional to the reduced mass difference, (M - M')/MM', where M and M' are masses of heavy and light isotope, respectively. The mass dependence clarifies that the isotope fractionations are originated from molecular vibration. The observed separation coefficient epsilon is 3.1x10(-3) for the pair of (40)Ca and (48)Ca. Productivity of enriched (48)Ca by crown-ether-resin was discussed as the function of the separation coefficient and the height equivalent to the theoretical plate.

40 CFR Table 1 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2013 CFR

2013-07-01

... Product Process Operation HAP Emission Limit (pounds of HAP loss per 1,000 square feet of leather processed) Existingsources Newsources 1. Upholstery Leather (≥4 grams add-on/square feet) 2.6 0.5 2. Upholstery Leather (square feet) 6.8 2.5 3. Water-resistant (≥5,000 Maeser Flexes)/Specialty...

40 CFR Table 1 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2012 CFR

2012-07-01

... Product Process Operation HAP Emission Limit (pounds of HAP loss per 1,000 square feet of leather processed) Existingsources Newsources 1. Upholstery Leather (≥4 grams add-on/square feet) 2.6 0.5 2. Upholstery Leather (square feet) 6.8 2.5 3. Water-resistant (≥5,000 Maeser Flexes)/Specialty...

40 CFR Table 1 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2014 CFR

2014-07-01

... Product Process Operation HAP Emission Limit (pounds of HAP loss per 1,000 square feet of leather processed) Existingsources Newsources 1. Upholstery Leather (≥4 grams add-on/square feet) 2.6 0.5 2. Upholstery Leather (square feet) 6.8 2.5 3. Water-resistant (≥5,000 Maeser Flexes)/Specialty...

40 CFR Table 1 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2011 CFR

2011-07-01

... Operation HAP Emission Limit (pounds of HAP loss per 1,000 square feet of leather processed) Existingsources Newsources 1. Upholstery Leather (≥4 grams add-on/square feet) 2.6 0.5 2. Upholstery Leather (square feet) 6.8 2.5 3. Water-resistant (≥5,000 Maeser Flexes)/Specialty Leather 5.6 4.9 4. Nonwater...

40 CFR Table 1 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2010 CFR

2010-07-01

... Operation HAP Emission Limit (pounds of HAP loss per 1,000 square feet of leather processed) Existingsources Newsources 1. Upholstery Leather (≥4 grams add-on/square feet) 2.6 0.5 2. Upholstery Leather (square feet) 6.8 2.5 3. Water-resistant (≥5,000 Maeser Flexes)/Specialty Leather 5.6 4.9 4. Nonwater...

Mass Independent Fractionation of Cadmium Isotopes During Thermal Ionization

NASA Astrophysics Data System (ADS)

Abouchami, W.; Galer, S. J.; Feldmann, H.; Schmitt, A. D.

2008-12-01

We have previously reported that Cd isotopes exhibit anomalous, non-mass dependent fractionation of odd versus even isotopes when measured by TIMS using silica gel-phosphoric acid activator. The deviation from mass dependent fractionation (MDF) on the odd masses 111 and 113 varies by fractions of a per-cent between runs. The effects cannot be explained by isobaric interferences, but seem, instead, to reflect mass independent fractionation (MIF) of Cd isotopes, much like that recently documented for Hg isotopes in natural systems (Bergquist and Blum, 2007). The absence of comparable Cd isotope anomalies in the ICP torch, and during extreme in-vacuo volatilization of Cd metal (Wombacher et al., 2004) conclusively implicates the silica gel activator in the process. So far, MIF has been documented for Cd, Zn and Pb isotopes when measured using the silica gel technique (Thirlwall, 2000; Schmitt et al., 2006; Manhes and Göpel, 2007). These MIF effects on Cd isotopes might perhaps be related to the non-mass dependence of nuclear volume with mass number, as described by Bigeleisen (1996) - also known as the "nuclear field shift". The MIF caused by the nuclear field shift results is a departure from MDF broadly characterized by a odd-even staggering with mass number. These effects have been quantified by Schauble (2007) who showed that the magnitude of the non-mass dependence for Hg and Tl isotopes lies in the ppm range for some simple reactions. Such MIF effects would appear, overall, far too small to account for our data, which require MIF offsets on the odd masses 111 and 113 approaching a per-cent. Moreover, an in-depth examination along the lines of Fujii et al. (2006) predicts tell-tale offsets for the even-even isotope pairs 114Cd/112Cd and 116Cd/112Cd as well, based upon the theory and the respective nuclear radii, but such accompanying offsets are unequivocally absent in our data. The odd-even isotope effects seen in our runs using silica gel activator are better

40 CFR 63.4541 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2012 CFR

2012-07-01

... no thinner and/or other additive, or cleaning material that contains organic HAP as determined... organic HAP content of coatings, thinners and/or other additives, and cleaning materials that are... volatile matter and use that value as a substitute for mass fraction of organic HAP. For reactive adhesives...

40 CFR 63.4541 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2014 CFR

2014-07-01

... no thinner and/or other additive, or cleaning material that contains organic HAP as determined... organic HAP content of coatings, thinners and/or other additives, and cleaning materials that are... volatile matter and use that value as a substitute for mass fraction of organic HAP. For reactive adhesives...

Propellant Mass Fraction Calculation Methodology for Launch Vehicles

NASA Technical Reports Server (NTRS)

Holt, James B.; Monk, Timothy S.

2009-01-01

Propellant Mass Fraction (pmf) calculation methods vary throughout the aerospace industry. While typically used as a means of comparison between competing launch vehicle designs, the actual pmf calculation method varies slightly from one entity to another. It is the purpose of this paper to present various methods used to calculate the pmf of a generic launch vehicle. This includes fundamental methods of pmf calculation which consider only the loaded propellant and the inert mass of the vehicle, more involved methods which consider the residuals and any other unusable propellant remaining in the vehicle, and other calculations which exclude large mass quantities such as the installed engine mass. Finally, a historic comparison is made between launch vehicles on the basis of the differing calculation methodologies.

[Optimized isolation and purification of non-typeable Haemophilus influenzae Haps protein].

PubMed

Li, Wan-yi; Kuang, Yu; Li, Ming-yuan; Yang, Yuan; Jiang, Zhong-hua; Yao, Feng; Chen, Chang-chun

2007-12-01

To optimize the isolation and purification conditions for Hap(s) protein of non-typeable Haemophilus influenzae. Hap(s) protein was purified by ammonium sulfate precipitation, dialysis desalting and Hitrap weak cation exchange columns of CM Sepharose Fast Flow. The condition of the elution was optimized for pH and ionic strength, the absorbance at 280 nm of the elution samples were detected, and the targeted protein band in the collected samples was observed by SDS-PAGE electrophoresis. The Hitrap ion exchange column was eluted with buffer 1, which resulted in a baseline distribution of absorbance at 280 nm. Buffer 2 elution of the column resulted in the presence of peak absorbance with trails, which was identified to be constituted by some low molecular weight bands by subsequent SDS-PAGE. In serial column elution with buffer 3 with different ionic strength, a peak absorbance was observed with the ionic strength of 100 mmol/L NaCl, and SDS-PAGE confirmed that the peak was generated by the target protein. No obvious peaks or bands in SDS-PAGE occurred with the other ionic strengths. The pH of the buffer only affect the elution of the irrelevant proteins rather than the Hap(s) protein, and elution with the buffer containing 100 mmol/L NaCl can be optimal for eluting the Hap(s) protein.

First observation of a mass independent isotopic fractionation in a condensation reaction

NASA Technical Reports Server (NTRS)

Thiemens, M. H.; Nelson, R.; Dong, Q. W.; Nuth, Joseph A., III

1994-01-01

Thiemens and Heidenreich (1983) first demonstrated that a chemically produced mass independent isotopic fractionation process could produce an isotopic composition which is identical to that observed in Allende inclusions. This raised the possibility that the meteoritic components could be produced by chemical, rather than nuclear processes. In order to develop a mechanistic model of the early solar system, it is important that relevant reactions be studied, particularly, those which may occur in the earliest condensation reactions. The isotopic results for isotopic fractionations associated with condensation processes are reported. A large mass independent isotopic fractionation is observed in one of the experiments.

24 CFR 983.205 - Term of HAP contract.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Term of HAP contract. 983.205 Section 983.205 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN DEVELOPMENT... DEVELOPMENT PROJECT-BASED VOUCHER (PBV) PROGRAM Housing Assistance Payments Contract § 983.205 Term of HAP...

24 CFR 983.205 - Term of HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Term of HAP contract. 983.205 Section 983.205 Housing and Urban Development Regulations Relating to Housing and Urban Development... DEVELOPMENT PROJECT-BASED VOUCHER (PBV) PROGRAM Housing Assistance Payments Contract § 983.205 Term of HAP...

40 CFR 63.4551 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2010 CFR

2010-07-01

... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR... organic HAP in coating, i, kg organic HAP per kg coating. For reactive adhesives as defined in § 63.4581... reactive adhesives as defined in § 63.4581, use the mass fraction of organic HAP that is emitted as...

40 CFR 63.3511 - What reports must I submit?

Code of Federal Regulations, 2010 CFR

2010-07-01

... calculation results for each rolling 12-month organic HAP emission rate during the 6-month reporting period... organic HAP, and the dates and time periods each was used. (ii) The calculation of the organic HAP content... coating suppliers or manufacturers, or test reports). (iii) The determination of mass fraction of organic...

40 CFR 63.4930 - What records must I keep?

Code of Federal Regulations, 2014 CFR

2014-07-01

... fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic... mass fraction of organic HAP for each coating, thinner, and cleaning material used during each... such as manufacturer's formulation data for the materials used, or test data used to determine the mass...

40 CFR 63.4930 - What records must I keep?

Code of Federal Regulations, 2011 CFR

2011-07-01

... fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic... mass fraction of organic HAP for each coating, thinner, and cleaning material used during each... such as manufacturer's formulation data for the materials used, or test data used to determine the mass...

40 CFR 63.4930 - What records must I keep?

Code of Federal Regulations, 2012 CFR

2012-07-01

... fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic... mass fraction of organic HAP for each coating, thinner, and cleaning material used during each... such as manufacturer's formulation data for the materials used, or test data used to determine the mass...

40 CFR 63.4930 - What records must I keep?

Code of Federal Regulations, 2013 CFR

2013-07-01

... fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic... mass fraction of organic HAP for each coating, thinner, and cleaning material used during each... such as manufacturer's formulation data for the materials used, or test data used to determine the mass...

STELLAR AND TOTAL BARYON MASS FRACTIONS IN GROUPS AND CLUSTERS SINCE REDSHIFT 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giodini, S.; Pierini, D.; Finoguenov, A.

2009-09-20

We investigate if the discrepancy between estimates of the total baryon mass fraction obtained from observations of the cosmic microwave background (CMB) and of galaxy groups/clusters persists when a large sample of groups is considered. To this purpose, 91 candidate X-ray groups/poor clusters at redshift 0.1 <= z <= 1 are selected from the COSMOS 2 deg{sup 2} survey, based only on their X-ray luminosity and extent. This sample is complemented by 27 nearby clusters with a robust, analogous determination of the total and stellar mass inside R {sub 500}. The total sample of 118 groups and clusters with zmore » <= 1 spans a range in M {sub 500} of {approx}10{sup 13}-10{sup 15} M {sub sun}. We find that the stellar mass fraction associated with galaxies at R {sub 500} decreases with increasing total mass as M {sup -0.37+}-{sup 0.04} {sub 500}, independent of redshift. Estimating the total gas mass fraction from a recently derived, high-quality scaling relation, the total baryon mass fraction (f {sup stars+gas} {sub 500} = f {sup stars} {sub 500} + f {sup gas} {sub 500}) is found to increase by {approx}25%, when M{sub 500} increases from (M) = 5 x 10{sup 13} M{sub sun} to (M) = 7 x 10{sup 14} M{sub sun}. After consideration of a plausible contribution due to intracluster light (11%-22% of the total stellar mass) and gas depletion through the hierarchical assembly process (10% of the gas mass), the estimated values of the total baryon mass fraction are still lower than the latest CMB measure of the same quantity (WMAP5), at a significance level of 3.3sigma for groups of (M) = 5 x 10{sup 13} M{sub sun}. The discrepancy decreases toward higher total masses, such that it is 1sigma at (M) = 7 x 10{sup 14} M{sub sun}. We discuss this result in terms of nongravitational processes such as feedback and filamentary heating.« less

40 CFR 63.4910 - What notifications must I submit?

Code of Federal Regulations, 2010 CFR

2010-07-01

..., accuracy, and completeness of the report. Such certifications must also comply with the requirements of 40... need to submit copies of any test reports. (i) Mass fraction of organic HAP for one coating, for one... mass of organic HAP contained in the waste materials for which you are claiming an allowance in...

40 CFR 63.4130 - What records must I keep?

Code of Federal Regulations, 2011 CFR

2011-07-01

... fraction of coating solids for each coating. If you conducted testing to determine mass fraction of organic... of the mass fraction of organic HAP for each coating, thinner, and cleaning material used during each... suppliers or manufacturers such as manufacturer's formulation data or test data used to determine the mass...

Interpreting spectral unmixing coefficients: From spectral weights to mass fractions

NASA Astrophysics Data System (ADS)

Grumpe, Arne; Mengewein, Natascha; Rommel, Daniela; Mall, Urs; Wöhler, Christian

2018-01-01

It is well known that many common planetary minerals exhibit prominent absorption features. Consequently, the analysis of spectral reflectance measurements has become a major tool of remote sensing. Quantifying the mineral abundances, however, is not a trivial task. The interaction between the incident light rays and particulate surfaces, e.g., the lunar regolith, leads to a non-linear relationship between the reflectance spectra of the pure minerals, the so-called ;endmembers;, and the surface's reflectance spectrum. It is, however, possible to transform the non-linear reflectance mixture into a linear mixture of single-scattering albedos of the Hapke model. The abundances obtained by inverting the linear single-scattering albedo mixture may be interpreted as volume fractions which are weighted by the endmember's extinction coefficient. Commonly, identical extinction coefficients are assumed throughout all endmembers and the obtained volume fractions are converted to mass fractions using either measured or assumed densities. In theory, the proposed method may cover different grain sizes if each grain size range of a mineral is treated as a distinct endmember. Here, we present a method to transform the mixing coefficients to mass fractions for arbitrary combinations of extinction coefficients and densities. The required parameters are computed from reflectance measurements of well defined endmember mixtures. Consequently, additional measurements, e.g., the endmember density, are no longer required. We evaluate the method based on laboratory measurements and various results presented in the literature, respectively. It is shown that the procedure transforms the mixing coefficients to mass fractions yielding an accuracy comparable to carefully calibrated laboratory measurements without additional knowledge. For our laboratory measurements, the square root of the mean squared error is less than 4.82 wt%. In addition, the method corrects for systematic effects

A double candidate survivable routing protocol for HAP network

NASA Astrophysics Data System (ADS)

He, Panfeng; Li, Chunyue; Ni, Shuyan

2016-11-01

To improve HAP network invulnerability, and at the same time considering the quasi-dynamic topology in HAP network, a simple and reliable routing protocol is proposed in the paper. The protocol firstly uses a double-candidate strategy for the next-node select to provide better robustness. Then during the maintenance stage, short hello packets instead of long routing packets are used only to check link connectivity in the quasi-dynamic HAP network. The route maintenance scheme based on short hello packets can greatly reduce link spending. Simulation results based on OPNET demonstrate the effectiveness of the proposed routing protocol.

On the conversion of tritium units to mass fractions for hydrologic applications

USGS Publications Warehouse

Stonestrom, David A.; Andraski, Brian J.; Cooper, Clay A.; Mayers, Charles J.; Michel, Robert L.

2013-01-01

We develop a general equation for converting laboratory-reported tritium levels, expressed either as concentrations (tritium isotope number fractions) or mass-based specific activities, to mass fractions in aqueous systems. Assuming that all tritium is in the form of monotritiated water simplifies the derivation and is shown to be reasonable for most environmental settings encountered in practice. The general equation is nonlinear. For tritium concentrations c less than 4.5×1012 tritium units (TU) - i.e. specific tritium activities11 Bq kg-1 - the mass fraction w of tritiated water is approximated to within 1 part per million by w ≈ c×2.22293×10-18, i.e. the conversion is linear for all practical purposes. Terrestrial abundances serve as a proxy for non-tritium isotopes in the absence of sample-specific data. Variation in the relative abundances of non-tritium isotopes in the terrestrial hydrosphere produces a minimum range for the mantissa of the conversion factor of [2.22287; 2.22300].

Mass-independent isotope fractionation of Mo, Ru, Cd, and Te

NASA Astrophysics Data System (ADS)

Fujii, T.; Moynier, F.; Albarède, F.

2006-12-01

The variation of the mean charge distribution in the nucleus with the neutron number of different isotopes induces a tenuous shift of the nuclear field. The mass fractionation induced during phase changes is irregular, notably with 'staggering' between odd and even masses, and becomes increasingly non-linear for neutron-rich isotopes. A strong correlation is observed between the deviation of the isotopic effects from the linear dependence with mass and the corresponding nuclear charge radii. We first demonstrated on a number of elements the existence of such mass-independent isotope fractionation in laboratory experiments of solvent extraction with a macrocyclic compound. The isotope ratios were analyzed by multiple-collector inductively coupled plasma mass spectrometry with a typical precision of <100 ppm. The isotopes of odd and even atomic masses are enriched in the solvent to an extent that closely follows the variation of their nuclear charge radii. The present results fit Bigeleisen's (1996) model, which is the standard mass-dependent theory modified to include a correction term named the nuclear field shift effect. For heavy elements like uranium, the mass-independent effect is important enough to dominate the mass-dependent effect. We subsequently set out to compare the predictions of Bigeleisen's theory with the isotopic anomalies found in meteorites. Some of these anomalies are clearly inconsistent with nucleosynthetic effects (either s- or r-processes). Isotopic variations of Mo and Ru in meteorites, especially in Allende (CV3), show a clear indication of nucleosynthetic components. However, the mass-independent anomaly of Ru observed in Murchison (CM2) is a remarkable exception which cannot be explained by the nucleosynthetic model, but fits the nuclear field shift theory extremely well. The abundances of the even atomic mass Te isotopes in the leachates of carbonaceous chondrites, Allende, Murchison, and Orgueil, fit a mass-dependent law well, but the

40 CFR 63.4291 - What are my options for meeting the emission limits?

Code of Federal Regulations, 2011 CFR

2011-07-01

... option. (b) Slashing. You must use the compliant material option to demonstrate that the mass fraction of... dyeing/finishing affected source. (1) Compliant material option. Demonstrate that the mass fraction of... paragraphs (4)(i) through (iv) of this paragraph. (i) The fraction of organic HAP applied in your dyeing...

40 CFR 63.4291 - What are my options for meeting the emission limits?

Code of Federal Regulations, 2014 CFR

2014-07-01

... option. (b) Slashing. You must use the compliant material option to demonstrate that the mass fraction of... dyeing/finishing affected source. (1) Compliant material option. Demonstrate that the mass fraction of... paragraphs (4)(i) through (iv) of this paragraph. (i) The fraction of organic HAP applied in your dyeing...

40 CFR 63.4291 - What are my options for meeting the emission limits?

Code of Federal Regulations, 2013 CFR

2013-07-01

... option. (b) Slashing. You must use the compliant material option to demonstrate that the mass fraction of... dyeing/finishing affected source. (1) Compliant material option. Demonstrate that the mass fraction of... paragraphs (4)(i) through (iv) of this paragraph. (i) The fraction of organic HAP applied in your dyeing...

40 CFR 63.4291 - What are my options for meeting the emission limits?

Code of Federal Regulations, 2012 CFR

2012-07-01

... option. (b) Slashing. You must use the compliant material option to demonstrate that the mass fraction of... dyeing/finishing affected source. (1) Compliant material option. Demonstrate that the mass fraction of... paragraphs (4)(i) through (iv) of this paragraph. (i) The fraction of organic HAP applied in your dyeing...

40 CFR 63.5340 - How do I determine the allowable HAP loss?

Code of Federal Regulations, 2010 CFR

2010-07-01

... appropriate HAP emission limit, expressed in pounds of HAP loss per 1,000 square feet of leather processed... the annual total of leather processed in 1,000's of square feet for each product process operation in... of square feet of leather processed in the previous 12 months in product process operation “i”. HAP...

40 CFR 63.5340 - How do I determine the allowable HAP loss?

Code of Federal Regulations, 2011 CFR

2011-07-01

... appropriate HAP emission limit, expressed in pounds of HAP loss per 1,000 square feet of leather processed... the annual total of leather processed in 1,000's of square feet for each product process operation in... of square feet of leather processed in the previous 12 months in product process operation “i”. HAP...

POLLUTION PREVENTION CASE STUDIES: LOW-VOC/HAP WOOD FURNITURE COATINGS

EPA Science Inventory

This article provides a brief profile of the wood furniture industry, discusses pollution prevention activities typically implemented, describes the four low-VOC/HAP coating technologies studied. and summarizes one case study for each of the low-VOC/HAP coating yechnologies inves...

Heteroaryldihydropyrimidine (HAP) and Sulfamoylbenzamide (SBA) Inhibit Hepatitis B Virus Replication by Different Molecular Mechanisms

PubMed Central

Zhou, Zheng; Hu, Taishan; Zhou, Xue; Wildum, Steffen; Garcia-Alcalde, Fernando; Xu, Zhiheng; Wu, Daitze; Mao, Yi; Tian, Xiaojun; Zhou, Yuan; Shen, Fang; Zhang, Zhisen; Tang, Guozhi; Najera, Isabel; Yang, Guang; Shen, Hong C.; Young, John A. T.; Qin, Ning

2017-01-01

Heteroaryldihydropyrimidine (HAP) and sulfamoylbenzamide (SBA) are promising non-nucleos(t)ide HBV replication inhibitors. HAPs are known to promote core protein mis-assembly, but the molecular mechanism of abnormal assembly is still elusive. Likewise, the assembly status of core protein induced by SBA remains unknown. Here we show that SBA, unlike HAP, does not promote core protein mis-assembly. Interestingly, two reference compounds HAP_R01 and SBA_R01 bind to the same pocket at the dimer-dimer interface in the crystal structures of core protein Y132A hexamer. The striking difference lies in a unique hydrophobic subpocket that is occupied by the thiazole group of HAP_R01, but is unperturbed by SBA_R01. Photoaffinity labeling confirms the HAP_R01 binding pose at the dimer-dimer interface on capsid and suggests a new mechanism of HAP-induced mis-assembly. Based on the common features in crystal structures we predict that T33 mutations generate similar susceptibility changes to both compounds. In contrast, mutations at positions in close contact with HAP-specific groups (P25A, P25S, or V124F) only reduce susceptibility to HAP_R01, but not to SBA_R01. Thus, HAP and SBA are likely to have distinctive resistance profiles. Notably, P25S and V124F substitutions exist in low-abundance quasispecies in treatment-naïve patients, suggesting potential clinical relevance. PMID:28205569

The HAP Complex Governs Fumonisin Biosynthesis and Maize Kernel Pathogenesis in Fusarium verticillioides.

PubMed

Ridenour, John B; Smith, Jonathon E; Bluhm, Burton H

2016-09-01

Contamination of maize ( Zea mays ) with fumonisins produced by the fungus Fusarium verticillioides is a global concern for food safety. Fumonisins are a group of polyketide-derived secondary metabolites linked to esophageal cancer and neural tube birth defects in humans and numerous toxicoses in livestock. Despite the importance of fumonisins in global maize production, the regulation of fumonisin biosynthesis during kernel pathogenesis is poorly understood. The HAP complex is a conserved, heterotrimeric transcriptional regulator that binds the consensus sequence CCAAT to modulate gene expression. Recently, functional characterization of the Hap3 subunit linked the HAP complex to the regulation of secondary metabolism and stalk rot pathogenesis in F. verticillioides . Here, we determine the involvement of HAP3 in fumonisin biosynthesis and kernel pathogenesis. Deletion of HAP3 suppressed fumonisin biosynthesis on both nonviable and live maize kernels and impaired pathogenesis in living kernels. Transcriptional profiling via RNA sequencing indicated that the HAP complex regulates at least 1,223 genes in F. verticillioides , representing nearly 10% of all predicted genes. Disruption of the HAP complex caused the misregulation of biosynthetic gene clusters underlying the production of secondary metabolites, including fusarins. Taken together, these results reveal that the HAP complex is a central regulator of fumonisin biosynthesis and kernel pathogenesis and works as both a positive and negative regulator of secondary metabolism in F. verticillioides .

Development of bioactive porous α-TCP/HAp beads for bone tissue engineering.

PubMed

Asaoka, Teruo; Ohtake, Shoji; Furukawa, Katsuko S; Tamura, Akito; Ushida, Takashi

2013-11-01

Porous beads of bioactive ceramics such as hydroxyapatite (HAp) and tribasic calcium phosphate (TCP) are considered a promising scaffold for cultivating bone cells. To realize this, α-TCP/HAp functionally graded porous beads are fabricated with two main purposes: to maintain the function of the scaffold with sufficient strength up to the growth of new bone, and is absorbed completely after the growth. HAp is a bioactive material that has both high strength and strong tissue-adhesive properties, but is not readily absorbed by the human body. On the contrary, α-TCP is highly bioabsorbable, resulting in a scaffold that is absorbed before it is completely replaced by bone. In this study, we produced porous, bead-shaped carriers as scaffolds for osteoblast culture. To control the solubility in vivo, the fabricated beads contained α-TCP at the center and HAp at the surface. Cell adaptability of these beads for bone tissue engineering was confirmed in vitro. It was found that α-TCP/HAp bead carriers exhibit low toxicity in the initial stages of cell seeding and cell adhesion. The presence of HAp in the composite bead form effectively increased ALP activity. In conclusion, it is suggested that these newly developed α-TCP/HAp beads are a promising tool for bone tissue engineering. Copyright © 2013 Wiley Periodicals, Inc.

Intracellular colocalization of HAP1/STBs with steroid hormone receptors and its enhancement by a proteasome inhibitor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fujinaga, Ryutaro; Takeshita, Yukio; Yoshioka, Kazuhiro

2011-07-15

The stigmoid body (STB) is a cytoplasmic inclusion containing huntingtin-associated protein 1 (HAP1), and HAP1/STB formation is induced by transfection of the HAP1 gene into cultured cells. In the present study, we examined the intracellular colocalization of HAP1/STBs with steroid hormone receptors (SHRs), including the androgen receptor (AR), estrogen receptor, glucocorticoid receptor (GR), and mineralocorticoid receptor, in COS-7 cells cotransfected with HAP1 and each receptor. We found that C-terminal ligand-binding domains of all SHRs had potential for colocalization with HAP1/STBs, whereas only AR and GR were clearly colocalized with HAP1/STBs when each full-length SHR was coexpressed with HAP1. In addition,more » it appeared that HAP1/STBs did not disrupt GR and AR functions because the receptors on HAP1/STBs maintained nuclear translocation activity in response to their specific ligands. When the cells were treated with a proteasome inhibitor, GR and AR localized outside HAP1/STBs translocated into the nucleus, whereas the receptors colocalized with HAP1/STBs persisted in their colocalization even after treatment with their ligands. Therefore, HAP1/STBs may be involved in cytoplasmic modifications of the nuclear translocation of GR and AR in a ubiquitin-proteasome system.« less

40 CFR Table 3 to Subpart Ggg of... - Soluble HAP

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 11 2010-07-01 2010-07-01 true Soluble HAP 3 Table 3 to Subpart GGG of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED... HAP Compound 1,1-Dimethylhydrazine. 1,4-Dioxane. Acetonitrile. Acetophenone. Diethyl sulfate. Dimethyl...

24 CFR 983.206 - HAP contract amendments (to add or substitute contract units).

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false HAP contract amendments (to add or... Contract § 983.206 HAP contract amendments (to add or substitute contract units). (a) Amendment to substitute contract units. At the discretion of the PHA and subject to all PBV requirements, the HAP contract...

HapMap tagSNP transferability in multiple populations: general guidelines

PubMed Central

Xing, Jinchuan; Witherspoon, David J.; Watkins, W. Scott; Zhang, Yuhua; Tolpinrud, Whitney; Jorde, Lynn B.

2008-01-01

This PDF receipt will only be used as the basis for generating PubMed Central (PMC) documents. PMC documents will be made available for review after conversion (approx. 2–3 weeks time). Any corrections that need to be made will be done at that time. No materials will be released to PMC without the approval of an author. Only the PMC documents will appear on PubMed Central -- this PDF Receipt will not appear on PubMed Central. Linkage disequilibrium (LD) has received much recent attention because of its value in localizing disease-causing genes. Due to the extensive LD between neighboring loci in the human genome, it is believed that a subset of the single nucleotide polymorphisms in a region (tagSNPs) can be selected to capture most of the remaining SNP variants. In this study, we examined LD patterns and HapMap tagSNP transferability in more than 300 individuals. A South Indian and an African Mbuti Pygmy population sample were included to evaluate the performance of HapMap tagSNPs in geographically distinct and genetically isolated populations. Our results show that HapMap tagSNPs selected with r2 >= 0.8 can capture more than 85% of the SNPs in populations that are from the same continental group. Combined tagSNPs from HapMap CEU and CHB+JPT serve as the best reference for the Indian sample. The HapMap YRI are a sufficient reference for tagSNP selection in the Pygmy sample. In addition to our findings, we reviewed over 25 recent studies of tagSNP transferability and propose a general guideline for selecting tagSNPs from HapMap populations. PMID:18482828

Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis.

PubMed

Chen, Fanjie; Hu, Han; Li, Zhonghua; Huang, Jiacheng; Cai, Xuwang; Wang, Chunmei; He, Qigai; Cao, Jiyue

2015-01-01

Cecropin B (CB) is a very effective natural antimicrobial peptide that has shown great potential for future antimicrobial drug development. HAPS_2096 is a Haemophilus parasuis gene that encodes the periplasmic substrate-binding protein of an ATP-binding cassette-type amino acid transporter. In this research, we constructed and verified an HAPS_2096 deletion mutant and a complementary HAPS_2096 mutant of H. parasuis JS0135. A bactericidal assay revealed that the HAPS_2096 deletion mutant was significantly more sensitive than the wild-type strain to 0.25-0.5 µg/ml CB. However, the gene complementation alleviated the CB sensitivity of the mutant. Immunoelectron microscopy observation following a 30-min treatment with a sublethal concentration of CB (0.25 μg/ml) revealed more extensive morphological damage in the mutant strain than in the wild-type strain. Hence, our results suggest that the HAPS_2096 gene contributes to H. parasuis resistance to CB. © 2015 S. Karger AG, Basel.

Navigation and Positioning System Using High Altitude Platforms Systems (HAPS)

NASA Astrophysics Data System (ADS)

Tsujii, Toshiaki; Harigae, Masatoshi; Harada, Masashi

Recently, some countries have begun conducting feasibility studies and R&D projects on High Altitude Platform Systems (HAPS). Japan has been investigating the use of an airship system that will function as a stratospheric platform for applications such as environmental monitoring, communications and broadcasting. If pseudolites were mounted on the airships, their GPS-like signals would be stable augmentations that would improve the accuracy, availability, and integrity of GPS-based positioning systems. Also, the sufficient number of HAPS can function as a positioning system independent of GPS. In this paper, a system design of the HAPS-based positioning system and its positioning error analyses are described.

Evolutionary history of the HAP2/GCS1 gene and sexual reproduction in metazoans.

PubMed

Steele, Robert E; Dana, Catherine E

2009-11-03

The HAP2/GCS1 gene first appeared in the common ancestor of plants, animals, and protists, and is required in the male gamete for fusion to the female gamete in the unicellular organisms Chlamydomonas and Plasmodium. We have identified a HAP2/GCS1 gene in the genome sequence of the sponge Amphimedon queenslandica. This finding provides a continuous evolutionary history of HAP2/GCS1 from unicellular organisms into the metazoan lineage. Divergent versions of the HAP2/GCS1 gene are also present in the genomes of some but not all arthropods. By examining the expression of the HAP2/GCS1 gene in the cnidarian Hydra, we have found the first evidence supporting the hypothesis that HAP2/GCS1 was used for male gamete fusion in the ancestor of extant metazoans and that it retains that function in modern cnidarians.

HDPE-Al2O3-HAp composites for biomedical applications: processing and characterizations.

PubMed

Nath, Shekhar; Bodhak, Subhadip; Basu, Bikramjit

2009-01-01

The objective of this work is to demonstrate how the stiffness, hardness, as well as the biocompatibility property, of bioinert high-density polyethylene (HDPE) can be significantly improved by the combined addition of both bioinert and bioactive ceramic fillers. For this purpose, different volume fractions of hydroxyapatite and alumina, limited to a total of 40 vol %, have been incorporated in HDPE matrix. All the hybrid composites and monolithic HDPE were developed under optimized hot pressing condition (130 degrees C, 0.5 h, 92 MPa pressure). The results of the mechanical property characterization reveal that higher elastic modulus (6.2 GPa) and improved hardness (226.5 MPa) could be obtained in the developed HDPE-20 vol %-HAp-20 vol % Al(2)O(3) composite. Under the selected fretting conditions against various counterbody materials (steel, Al(2)O(3), and ZrO(2)), an extremely low COF of (0.07-0.11) and higher wear resistance (order of 10(-6) mm(3)/Nm) are obtained with the HDPE/20 vol % HAp/20 vol % Al(2)O(3) composite in both air and simulated body fluid environment. Importantly, in-vitro cell culture study using L929 fibroblast cells confirms favorable cell adhesion properties in the developed hybrid composite. (c) 2008 Wiley Periodicals, Inc.

24 CFR 982.611 - Group home: Lease and HAP contract.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Group home: Lease and HAP contract. 982.611 Section 982.611 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN... Types Group Home § 982.611 Group home: Lease and HAP contract. For assistance in a group home, there is...

24 CFR 982.611 - Group home: Lease and HAP contract.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Group home: Lease and HAP contract. 982.611 Section 982.611 Housing and Urban Development Regulations Relating to Housing and Urban... Types Group Home § 982.611 Group home: Lease and HAP contract. For assistance in a group home, there is...

24 CFR 982.611 - Group home: Lease and HAP contract.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Group home: Lease and HAP contract. 982.611 Section 982.611 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN... Types Group Home § 982.611 Group home: Lease and HAP contract. For assistance in a group home, there is...

Molecular Characterization of Hap Complex Components Responsible for Methanol-Inducible Gene Expression in the Methylotrophic Yeast Candida boidinii

PubMed Central

Oda, Saori; Yurimoto, Hiroya; Nitta, Nobuhisa; Sasano, Yu

2015-01-01

We identified genes encoding components of the Hap complex, CbHAP2, CbHAP3, and CbHAP5, as transcription factors regulating methanol-inducible gene expression in the methylotrophic yeast Candida boidinii. We found that the Cbhap2Δ, Cbhap3Δ, and Cbhap5Δ gene-disrupted strains showed severe growth defects on methanol but not on glucose and nonfermentable carbon sources such as ethanol and glycerol. In these disruptants, the transcriptional activities of methanol-inducible promoters were significantly decreased compared to those of the wild-type strain, indicating that CbHap2p, CbHap3p, and CbHap5p play indispensable roles in methanol-inducible gene expression. Further molecular and biochemical analyses demonstrated that CbHap2p, CbHap3p, and CbHap5p localized to the nucleus and bound to the promoter regions of methanol-inducible genes regardless of the carbon source, and heterotrimer formation was suggested to be necessary for binding to DNA. Unexpectedly, distinct from Saccharomyces cerevisiae, the Hap complex functioned in methanol-specific induction rather than glucose derepression in C. boidinii. Our results shed light on a novel function of the Hap complex in methanol-inducible gene expression in methylotrophic yeasts. PMID:25595445

Enhanced bone regeneration composite scaffolds of PLLA/β-TCP matrix grafted with gelatin and HAp.

PubMed

Wang, Jie-Lin; Chen, Qian; Du, Bei-Bei; Cao, Lu; Lin, Hong; Fan, Zhong-Yong; Dong, Jian

2018-06-01

The composite polylactide PLLA/β-TCP scaffolds were fabricated by solution casting and were coated with gelatin/hydroxyapatite (Gel/HAp) to improve the biological properties of the composite scaffolds. The Gel/HAp mixture was prepared using an in situ reaction, and a grafting-coating method was used to increase the efficiency of coating the PLLA/β-TCP matrix with Gel/HAp. First, free amino groups were introduced by 1,6-hexanediamine to aminolyze the PLLA/β-TCP matrix surface. Second, glutaraldehyde was coupled to Gel/HAp as a crosslinking agent. The structure and properties of Gel/HAp-modified PLLA/β-TCP films were characterized by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and water contact angle measurements (WCA). The experimental results show that 23 wt% HAp was uniformly dispersed in the gelatin coating by in situ synthesis. The Gel/HAp composite coating was successfully immobilized on the aminolyzed PLLA/β-TCP surface via a chemical grafting method, which promoted a lower degradation rate and was more hydrophilic than a physical grafting method. The Gel/HAp composite coating adhered tightly and homogeneously to the hydrophobic PLLA/β-TCP surface. Moreover, mouse embryo osteoblast precursor (MC3T3-E1) cells grown on the scaffolds were behaviorally and morphologically characterized. The results indicated that the Gel/HAp composite coating was favorable for the attachment and proliferation of preosteoblasts and that Gel/HAp-NH-PLLA/β-TCP would be a candidate scaffold for bone repair. Copyright © 2018 Elsevier B.V. All rights reserved.

In Vivo Mass-independent Fractionation of Mercury Isotopes in Fish

NASA Astrophysics Data System (ADS)

Das, R.; Odom, L. A.

2008-12-01

Recent experimental work and analyses of natural samples have revealed both mass-dependent and mass- independent isotope fractionation effects in mercury. These findings portend new avenues toward understanding the global mercury cycle. It has been shown experimentally that photo reduction of Hg+2 and methylmercury in water with concomitant release of the reduced, gaseous species Hg° results in the residual methylmercury possessing a mass-independent isotope effect. This effect is a relative enrichment of isotopes 199Hg and 201Hg over the even mass number isotopes when compared to the mercury standard NIST SRM3133. Large mass independent fractionation (MIF) effects (Δ199Hg values of a few ‰) have been found in mercury in fish and interpreted as isotope effects inherited from the water. To evaluate the possibility that MIF might be produced within the fish, we have analyzed 38 samples that include zooplankton and twelve different species of fish from a single lake collected over a 2-month time period for mercury isotopic compositions. Trophic levels of the same fish specimens had previously been determined from stomach contents and nitrogen isotopes. Zooplankton in the lake contain mercury with Δ199Hg and Δ201Hg values of +0.43 (±0.07) and +0.44 (±0.07) respectively. Among the fish species there is a striking correspondence between trophic level and Δ199Hg and Δ201Hg values for primary, secondary, and tertiary consumers. The Δ199Hg values ranges over ~1‰ from ~+0.4 in zooplankton, juvenile bluegill and several other small fishes to Δ199Hg = + 1.36 for the Florida gar that is the top predator fish in the lake. These observations indicate that the MIF effect, rather than being an artifact of the water column is produced in vivo. Partial separation of 199Hg and 201Hg from isotopes of even neutron number can be achieved by the magnetic isotope effect in reactions involving sufficiently long-lived intermediate free radicals, where nuclear - electron

Volatile and semivolatile organic compounds in laboratory ...

EPA Pesticide Factsheets

Speciated volatile organic compounds (VOCs) and organic fine particulate matter (PM2.5) mass emission factors were determined from laboratory peat fire experiments. Peat samples originated from two wildlife reserves located near the coast of North Carolina, U.S. Gas and particulate organics were quantified by gas chromatography/mass spectrometry and by high pressure liquid chromatography. Hazardous air pollutants (HAPs) accounted for a large fraction (~60 %) of the speciated VOC emissions from peat burning, including large contributions of acetaldehyde, formaldehyde, benzene, toluene, and chloromethane. Speciated organic PM2.5 mass was dominated by the following compound classes: organic acids, levoglucosan, n-alkanes, and n-alkenes. Emission factors for PM2.5 organic acids including n-alkanoic acids, n-alkenoic acids, n-alkanedioic acids, and aromatic acids were reported for the first time for peat burning, representing the largest fraction of organic carbon (OC) mass (11-12 %) of all speciated compound classes measured in this work. Levoglucosan contributed 2-3 % of the OC mass, while methoxyphenols represented 0.2-0.3 % of the OC mass on a carbon mass basis. Retene was the most abundant particulate phase polycyclic aromatic hydrocarbon. Total HAP VOC and particulate polycyclic aromatic hydrocarbon emissions from a 2008 peat wildfire in North Carolina were estimated, suggesting that peat fires can contribute a large fraction of state-wide HAP emissions. This p

Volatile and semivolatile organic compounds in laboratory peat fire emissions

NASA Astrophysics Data System (ADS)

George, Ingrid J.; Black, Robert R.; Geron, Chris D.; Aurell, Johanna; Hays, Michael D.; Preston, William T.; Gullett, Brian K.

2016-05-01

In this study, volatile and semi-volatile organic compound (VOCs and SVOCs) mass emission factors were determined from laboratory peat fire experiments. The peat samples originated from two National Wildlife Refuges on the coastal plain of North Carolina, U.S.A. Gas- and particle-phase organic compounds were quantified by gas chromatography-mass spectrometry and by high pressure liquid chromatography. Hazardous air pollutants (HAPs) accounted for a large fraction (∼60%) of the speciated VOC emissions from peat burning, including large contributions of acetaldehyde, formaldehyde, benzene, toluene, and chloromethane. In the fine particle mass (PM2.5), the following organic compound classes were dominant: organic acids, levoglucosan, n-alkanes, and n-alkenes. Emission factors for the organic acids in PM2.5 including n-alkanoic acids, n-alkenoic acids, n-alkanedioic acids, and aromatic acids were reported for the first time for peat burning, representing the largest fraction of organic carbon (OC) mass (11-12%) of all speciated compound classes measured in this work. Levoglucosan contributed to 2-3% of the OC mass, while methoxyphenols represented 0.2-0.3% of the OC mass on a carbon mass basis. Retene was the most abundant particulate phase polycyclic aromatic hydrocarbon (PAH). Total HAP VOC and particulate PAH emissions from a 2008 peat wildfire in North Carolina were estimated, suggesting that peat fires can contribute a large fraction of state-wide HAP emissions.

40 CFR 63.4541 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2010 CFR

2010-07-01

... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR... volatile matter and use that value as a substitute for mass fraction of organic HAP. For reactive adhesives... not have to count it. For reactive adhesives in which some of the HAP react to form solids and are not...

40 CFR 63.3951 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2010 CFR

2010-07-01

... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR... coating. For reactive adhesives as defined in § 63.3981, use the mass fraction of organic HAP that is... organic HAP per kg thinner and/or other additive. For reactive adhesives as defined in § 63.3981, use the...

Study on calcifying treatments of hydroxyapatite (HAp) using calcifying promotion solution

NASA Astrophysics Data System (ADS)

Wakaki, Moriaki; Yazaki, Syungo; Sunada, Yoshikazu

2009-02-01

Apatite is expected to be a useful material for artificial bones in surgery and artificial dental roots in dentistry. In particular, studies have recently been conducted into the reconstruction of teeth using Hydroxyapatite (HAp), and several supplements such as gum have become popular for keeping teeth in good condition. However, the decalcifying and calcifying processes are still not well understood. The aim of this research is to study the decalcifying and calcifying mechanisms of HAp. Specifically, the calcifying treatments were carried out on sintered pellets of HAp without pores using Phosphate Acid Maltodextrin (PMD) and Xylitol calcifying promotion agents. A natural calcifying liquid which simulates the situation within a human mouth was used as a reference. SEM, EDX, X-ray, IR and Raman measurements were used for the characterization of structures, morphologies, formed elements and physical properties. It was confirmed that a precursor material OCP was grown on the HAp pellet by the calcification treatment using each promotion agent.

A DIRECT MEASUREMENT OF THE BARYONIC MASS FUNCTION OF GALAXIES AND IMPLICATIONS FOR THE GALACTIC BARYON FRACTION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Papastergis, Emmanouil; Huang, Shan; Giovanelli, Riccardo

We use both an H I-selected and an optically selected galaxy sample to directly measure the abundance of galaxies as a function of their 'baryonic' mass (stars + atomic gas). Stellar masses are calculated based on optical data from the Sloan Digital Sky Survey and atomic gas masses are calculated using atomic hydrogen (H I) emission line data from the Arecibo Legacy Fast ALFA survey. By using the technique of abundance matching, we combine the measured baryonic function of galaxies with the dark matter halo mass function in a {Lambda}CDM universe, in order to determine the galactic baryon fraction asmore » a function of host halo mass. We find that the baryon fraction of low-mass halos is much smaller than the cosmic value, even when atomic gas is taken into account. We find that the galactic baryon deficit increases monotonically with decreasing halo mass, in contrast with previous studies which suggested an approximately constant baryon fraction at the low-mass end. We argue that the observed baryon fractions of low-mass halos cannot be explained by reionization heating alone, and that additional feedback mechanisms (e.g., supernova blowout) must be invoked. However, the outflow rates needed to reproduce our result are not easily accommodated in the standard picture of galaxy formation in a {Lambda}CDM universe.« less

Chromosomal Expression of the Haemophilus influenzae Hap Autotransporter Allows Fine-Tuned Regulation of Adhesive Potential via Inhibition of Intermolecular Autoproteolysis

PubMed Central

Fink, Doran L.; St. Geme III, Joseph W.

2003-01-01

The Haemophilus influenzae Hap autotransporter is a nonpilus adhesin that promotes adherence to respiratory epithelial cells and selected extracellular matrix proteins and facilitates bacterial aggregation and microcolony formation. Hap consists of a 45-kDa outer membrane translocator domain called Hapβ and a 110-kDa extracellular passenger domain called HapS. All adhesive activity resides within HapS, which also contains protease activity and directs its own secretion from the bacterial cell surface via intermolecular autoproteolysis. In the present study, we sought to determine the relationship between the magnitude of Hap expression, the efficiency of Hap autoproteolysis, and the level of Hap-mediated adherence and aggregation. We found that a minimum threshold of Hap precursor was required for autoproteolysis and that this threshold approximated expression of Hap from a chromosomal allele, as occurs in H. influenzae clinical isolates. Chromosomal expression of wild-type Hap was sufficient to promote significant adherence to epithelial cells and extracellular matrix proteins, and adherence was enhanced substantially by inhibition of autoproteolysis. In contrast, chromosomal expression of Hap was sufficient to promote bacterial aggregation only when autoproteolysis was inhibited, indicating that the threshold for Hap-mediated aggregation is above the threshold for autoproteolysis. These results highlight the critical role of autoproteolysis and an intermolecular mechanism of cleavage in controlling the diverse adhesive activities of Hap. PMID:12591878

A comparison of cataloged variation between International HapMap Consortium and 1000 Genomes Project data.

PubMed

Buchanan, Carrie C; Torstenson, Eric S; Bush, William S; Ritchie, Marylyn D

2012-01-01

Since publication of the human genome in 2003, geneticists have been interested in risk variant associations to resolve the etiology of traits and complex diseases. The International HapMap Consortium undertook an effort to catalog all common variation across the genome (variants with a minor allele frequency (MAF) of at least 5% in one or more ethnic groups). HapMap along with advances in genotyping technology led to genome-wide association studies which have identified common variants associated with many traits and diseases. In 2008 the 1000 Genomes Project aimed to sequence 2500 individuals and identify rare variants and 99% of variants with a MAF of <1%. To determine whether the 1000 Genomes Project includes all the variants in HapMap, we examined the overlap between single nucleotide polymorphisms (SNPs) genotyped in the two resources using merged phase II/III HapMap data and low coverage pilot data from 1000 Genomes. Comparison of the two data sets showed that approximately 72% of HapMap SNPs were also found in 1000 Genomes Project pilot data. After filtering out HapMap variants with a MAF of <5% (separately for each population), 99% of HapMap SNPs were found in 1000 Genomes data. Not all variants cataloged in HapMap are also cataloged in 1000 Genomes. This could affect decisions about which resource to use for SNP queries, rare variant validation, or imputation. Both the HapMap and 1000 Genomes Project databases are useful resources for human genetics, but it is important to understand the assumptions made and filtering strategies employed by these projects.

Hazardous air pollutant (HAP) emission characterization of sewage treatment facilities in Korea.

PubMed

Kang, Kyoung-Hee; Dong, Jong-In

2010-04-01

Until recently, nearly all sewage treatment-related regulations and researches have focused on the removal of the conventional and toxic pollutants from liquid effluents. The discharge of toxic compounds to the atmosphere has been implicitly regarded as a way of removal or destruction. During sewage treatment, the fate mechanism of volatilization/stripping, sorption and biotransformation primarily determines the fate of volatile HAPs. The objectives of this study are to investigate the emission characteristics of HAPs, which are generated from the liquid surface of sewage treatment facilities, by using an emission isolation flux chamber. HAP emissions increased at the inlet of the aerobic chamber during summer due to the relatively high atmospheric temperature. The percent ratio of flux for toluene reached its peak in winter, accounting for 33.6-34.2% of the total, but decreased to 25.1-28.6% in summer. In autumn, trichloroethene (TCE) was the highest, recording 17.6-18.1%, with chloroform and toluene showing similar levels. It seems that the ratio of chlorinated hydrocarbons increases in both summer and autumn because the chamber temperature during that time is higher than winter. This study is the initial study to investigate the emission characteristics of volatile HAPs emitted from domestic sewage treatment facilities to the air in Korea. Therefore, the isolation flux chamber will be used as an emission estimations tool to measure HAPs from sewage treatment facilities and may be applied to develop the emission factor and national source inventory of HAPs.

In vitro and in vivo tests of PLA/d-HAp nanocomposite

NASA Astrophysics Data System (ADS)

Thom Nguyen, Thi; Hoang, Thai; Mao Can, Van; Son Ho, Anh; Hai Nguyen, Song; Thu Trang Nguyen, Thi; Pham, Thi Nam; Phuong Nguyen, Thu; Le Hien Nguyen, Thi; Thanh Dinh Thi, Mai

2017-12-01

The bioactivity of the PLA/d-HAp nanocomposite with 30 wt.% d-HAp was evaluated by in vitro tests and indicated that after 7 immersion days in SBF solution, PLA amorphous part was hydrolyzed and PLA crystal part was remained. The formation of apatite on the surface of the material was observed. The in vivo test results of PLA/d-HAp nanocomposite (70/30 wt/wt) on femur of dogs displayed that 3 months after grafting, the materials did not induce any osteitis, osteomyelitis or structural abnormalities. The histological and x-ray image demonstrated a growth of the bone into the material area, while osteitis and osteomyelitis were not observed.

Diversity Performance Analysis on Multiple HAP Networks.

PubMed

Dong, Feihong; Li, Min; Gong, Xiangwu; Li, Hongjun; Gao, Fengyue

2015-06-30

One of the main design challenges in wireless sensor networks (WSNs) is achieving a high-data-rate transmission for individual sensor devices. The high altitude platform (HAP) is an important communication relay platform for WSNs and next-generation wireless networks. Multiple-input multiple-output (MIMO) techniques provide the diversity and multiplexing gain, which can improve the network performance effectively. In this paper, a virtual MIMO (V-MIMO) model is proposed by networking multiple HAPs with the concept of multiple assets in view (MAV). In a shadowed Rician fading channel, the diversity performance is investigated. The probability density function (PDF) and cumulative distribution function (CDF) of the received signal-to-noise ratio (SNR) are derived. In addition, the average symbol error rate (ASER) with BPSK and QPSK is given for the V-MIMO model. The system capacity is studied for both perfect channel state information (CSI) and unknown CSI individually. The ergodic capacity with various SNR and Rician factors for different network configurations is also analyzed. The simulation results validate the effectiveness of the performance analysis. It is shown that the performance of the HAPs network in WSNs can be significantly improved by utilizing the MAV to achieve overlapping coverage, with the help of the V-MIMO techniques.

Diversity Performance Analysis on Multiple HAP Networks

PubMed Central

Dong, Feihong; Li, Min; Gong, Xiangwu; Li, Hongjun; Gao, Fengyue

2015-01-01

One of the main design challenges in wireless sensor networks (WSNs) is achieving a high-data-rate transmission for individual sensor devices. The high altitude platform (HAP) is an important communication relay platform for WSNs and next-generation wireless networks. Multiple-input multiple-output (MIMO) techniques provide the diversity and multiplexing gain, which can improve the network performance effectively. In this paper, a virtual MIMO (V-MIMO) model is proposed by networking multiple HAPs with the concept of multiple assets in view (MAV). In a shadowed Rician fading channel, the diversity performance is investigated. The probability density function (PDF) and cumulative distribution function (CDF) of the received signal-to-noise ratio (SNR) are derived. In addition, the average symbol error rate (ASER) with BPSK and QPSK is given for the V-MIMO model. The system capacity is studied for both perfect channel state information (CSI) and unknown CSI individually. The ergodic capacity with various SNR and Rician factors for different network configurations is also analyzed. The simulation results validate the effectiveness of the performance analysis. It is shown that the performance of the HAPs network in WSNs can be significantly improved by utilizing the MAV to achieve overlapping coverage, with the help of the V-MIMO techniques. PMID:26134102

40 CFR Table 4 to Subpart Oooo of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2011 CFR

2011-07-01

... by mass 1. Toluene 108-88-3 1.0 Toluene. 2. Xylene(s) 1330-20-7 1.0 Xylenes, ethylbenzene. 3. Hexane 110-54-3 0.5 n-hexane. 4. n-Hexane 110-54-3 1.0 n-hexane. 5. Ethylbenzene 100-41-4 1.0 Ethylbenzene. 6...

40 CFR Table 4 to Subpart Oooo of... - Default Organic HAP Mass Fraction for Solvents and Solvent Blends

Code of Federal Regulations, 2010 CFR

2010-07-01

... by mass 1. Toluene 108-88-3 1.0 Toluene. 2. Xylene(s) 1330-20-7 1.0 Xylenes, ethylbenzene. 3. Hexane 110-54-3 0.5 n-hexane. 4. n-Hexane 110-54-3 1.0 n-hexane. 5. Ethylbenzene 100-41-4 1.0 Ethylbenzene. 6...

Homeowners Assistance Program (HAP)

DTIC Science & Technology

1992-06-26

Assistance Program (HAP) References: (a) DoD Instruction 4165.50, "Administration and Operation of the Homeowners Assistance Program ," February 11, 1972...hereby canceled) (b) DoD Directive 5100.54, "Homeowners Assistance Program ," December 29, 1967 (hereby canceled) (c) Section 1013 of Public Law 89-754...Defense Program and annual budgets for the Homeowners Assistance Fund, Defense. m. Publish regulations and forms, subject to review by . the Assistant

Source contributions to black carbon mass fractions in aerosol particles over the northwestern Pacific

NASA Astrophysics Data System (ADS)

Koga, Seizi; Maeda, Takahisa; Kaneyasu, Naoki

Aerosol particle number size distributions above 0.3 μm in diameter and black carbon mass concentrations in aerosols were observed on Chichi-jima of the Ogasawara Islands in the northwestern Pacific from January 2000 to December 2002. Chichi-jima is suitable to observe polluted air masses from East Asia in winter and clean air masses over the western North Pacific in summer. In winter, aerosols over Chichi-jima were strongly affected by anthropogenic emissions in East Asia. The form of energy consumption in East Asia varies in various regions. Hence, each source region is expected to be characterized by an individual black carbon mass fraction. A three-dimensional Eulerian transport model was used to estimate contribution rates to air pollutants from each source region in East Asia. Because the Miyake-jima eruption began at the end of June 2000, the influence of smokes from Miyake-jima was also considered in the model calculation. The results of model calculations represent what must be noticed about smokes from volcanoes including Miyake-jima to interpret temporal variations of sulfur compounds over the northwestern Pacific. To evaluate black carbon mass fractions in anthropogenic aerosols as a function of source region, the relationships between the volume concentration of aerosol particles and the black carbon mass concentration in the winter were classified under each source region in East Asia. Consequently, the black carbon mass fractions in aerosols from China, Japan and the Korean Peninsula, and other regions were estimated to be 9-13%, 5-7%, and 4-5%, respectively.

Mass-loss rates, ionization fractions, shock velocities, and magnetic fields of stellar jets

NASA Technical Reports Server (NTRS)

Hartigan, Patrick; Morse, Jon A.; Raymond, John

1994-01-01

In this paper we calculate emission-line ratios from a series of planar radiative shock models that cover a wide range of shock velocities, preshock densities, and magnetic fields. The models cover the initial conditions relevant to stellar jets, and we show how to estimate the ionization fractions and shock velocities in jets directly from observations of the strong emission lines in these flows. The ionization fractions in the HH 34, HH 47, and HH 111 jets are approximately 2%, considerably smaller than previous estimates, and the shock velocities are approximately 30 km/s. For each jet the ionization fractions were found from five different line ratios, and the estimates agree to within a factor of approximately 2. The scatter in the estimates of the shock velocities is also small (+/- 4 km/s). The low ionization fractions of stellar jets imply that the observed electron densities are much lower than the total densities, so the mass-loss rates in these flows are correspondingly higher (approximately greater than 2 x 10(exp -7) solar mass/yr). The mass-loss rates in jets are a significant fraction (1%-10%) of the disk accretion rates onto young stellar objects that drive the outflows. The momentum and energy supplied by the visible portion of a typical stellar jet are sufficient to drive a weak molecular outflow. Magnetic fields in stellar jets are difficult to measure because the line ratios from a radiative shock with a magnetic field resemble those of a lower velocity shock without a field. The observed line fluxes can in principle indicate the strength of the field if the geometry of the shocks in the jet is well known.

A comparison of cataloged variation between International HapMap Consortium and 1000 Genomes Project data

PubMed Central

Buchanan, Carrie C; Torstenson, Eric S; Bush, William S

2012-01-01

Background Since publication of the human genome in 2003, geneticists have been interested in risk variant associations to resolve the etiology of traits and complex diseases. The International HapMap Consortium undertook an effort to catalog all common variation across the genome (variants with a minor allele frequency (MAF) of at least 5% in one or more ethnic groups). HapMap along with advances in genotyping technology led to genome-wide association studies which have identified common variants associated with many traits and diseases. In 2008 the 1000 Genomes Project aimed to sequence 2500 individuals and identify rare variants and 99% of variants with a MAF of <1%. Methods To determine whether the 1000 Genomes Project includes all the variants in HapMap, we examined the overlap between single nucleotide polymorphisms (SNPs) genotyped in the two resources using merged phase II/III HapMap data and low coverage pilot data from 1000 Genomes. Results Comparison of the two data sets showed that approximately 72% of HapMap SNPs were also found in 1000 Genomes Project pilot data. After filtering out HapMap variants with a MAF of <5% (separately for each population), 99% of HapMap SNPs were found in 1000 Genomes data. Conclusions Not all variants cataloged in HapMap are also cataloged in 1000 Genomes. This could affect decisions about which resource to use for SNP queries, rare variant validation, or imputation. Both the HapMap and 1000 Genomes Project databases are useful resources for human genetics, but it is important to understand the assumptions made and filtering strategies employed by these projects. PMID:22319179

The ATLAS3D project - XX. Mass-size and mass-σ distributions of early-type galaxies: bulge fraction drives kinematics, mass-to-light ratio, molecular gas fraction and stellar initial mass function

NASA Astrophysics Data System (ADS)

Cappellari, Michele; McDermid, Richard M.; Alatalo, Katherine; Blitz, Leo; Bois, Maxime; Bournaud, Frédéric; Bureau, M.; Crocker, Alison F.; Davies, Roger L.; Davis, Timothy A.; de Zeeuw, P. T.; Duc, Pierre-Alain; Emsellem, Eric; Khochfar, Sadegh; Krajnović, Davor; Kuntschner, Harald; Morganti, Raffaella; Naab, Thorsten; Oosterloo, Tom; Sarzi, Marc; Scott, Nicholas; Serra, Paolo; Weijmans, Anne-Marie; Young, Lisa M.

2013-07-01

) and dwarf irregulars (Im), respectively. We use dynamical models to analyse our kinematic maps. We show that σe traces the bulge fraction, which appears to be the main driver for the observed trends in the dynamical (M/L)JAM and in indicators of the (M/L)pop of the stellar population like Hβ and colour, as well as in the molecular gas fraction. A similar variation along contours of σe is also observed for the mass normalization of the stellar initial mass function (IMF), which was recently shown to vary systematically within the ETGs' population. Our preferred relation has the form log _{10} [(M/L)_stars/(M/L)_Salp]=a+b× log _{10}({σ _e}/130 {km s^{-1}}) with a = -0.12 ± 0.01 and b = 0.35 ± 0.06. Unless there are major flaws in all stellar population models, this trend implies a transition of the mean IMF from Kroupa to Salpeter in the interval log _{10}({σ _e}/{km s}^{-1})≈ 1.9-2.5 (or {σ _e}≈ 90-290 km s-1), with a smooth variation in between, consistently with what was shown in Cappellari et al. The observed distribution of galaxy properties on the MP provides a clean and novel view for a number of previously reported trends, which constitute special two-dimensional projections of the more general four-dimensional parameters trends on the MP. We interpret it as due to a combination of two main effects: (i) an increase of the bulge fraction, which increases σe, decreases Re, and greatly enhance the likelihood for a galaxy to have its star formation quenched, and (ii) dry merging, increasing galaxy mass and Re by moving galaxies along lines of roughly constant σe (or steeper), while leaving the population nearly unchanged.

Inappropriate left ventricular mass and poor outcomes in patients with angina pectoris and normal ejection fraction.

PubMed

Huang, Bao-Tao; Peng, Yong; Liu, Wei; Zhang, Chen; Huang, Fang-Yang; Wang, Peng-Ju; Zuo, Zhi-Liang; Liao, Yan-Biao; Chai, Hua; Li, Qiao; Zhao, Zhen-Gang; Luo, Xiao-Lin; Ren, Xin; Huang, Kai-Sen; Meng, Qing-Tao; Chen, Chi; Huang, De-Jia; Chen, Mao

2015-03-01

Although inappropriate left ventricular mass has been associated with clustered cardiac geometric and functional abnormalities, its predictive value in patients with coronary artery disease is still unknown. This study examined the association of inappropriate left ventricular mass with clinical outcomes in patients with angina pectoris and normal ejection fraction. Consecutive patients diagnosed with angina pectoris whose ejection fraction was normal were recruited from 2008 to 2012. Inappropriate left ventricular mass was determined when the ratio of actual left ventricular mass to the predicted one exceeded 150%. The primary endpoint was a composite of all-cause death, nonfatal myocardial infarction, and nonfatal stroke. Clinical outcomes between the inappropriate and appropriate left ventricular mass group were compared before and after propensity matching. Of the total of 1515 participants, 18.3% had inappropriate left ventricular mass. Patients with inappropriate left ventricular mass had a higher composite event rate compared with those with appropriate left ventricular mass (11.2 vs. 6.6%, P=0.010). Multivariate Cox regression analyses showed that inappropriate left ventricular mass was an independent risk factor for adverse events (adjusted hazard ratio, 1.59; 95% confidence interval, 1.03-2.45; P=0.035). The worse outcome in patients with inappropriate left ventricular mass was further validated in a propensity matching cohort and patients with the traditional definition of left ventricular hypertrophy. Inappropriate left ventricular mass was associated with an increased risk of adverse events in patients with angina pectoris and normal ejection fraction.

SimHap GUI: An intuitive graphical user interface for genetic association analysis

PubMed Central

Carter, Kim W; McCaskie, Pamela A; Palmer, Lyle J

2008-01-01

Background Researchers wishing to conduct genetic association analysis involving single nucleotide polymorphisms (SNPs) or haplotypes are often confronted with the lack of user-friendly graphical analysis tools, requiring sophisticated statistical and informatics expertise to perform relatively straightforward tasks. Tools, such as the SimHap package for the R statistics language, provide the necessary statistical operations to conduct sophisticated genetic analysis, but lacks a graphical user interface that allows anyone but a professional statistician to effectively utilise the tool. Results We have developed SimHap GUI, a cross-platform integrated graphical analysis tool for conducting epidemiological, single SNP and haplotype-based association analysis. SimHap GUI features a novel workflow interface that guides the user through each logical step of the analysis process, making it accessible to both novice and advanced users. This tool provides a seamless interface to the SimHap R package, while providing enhanced functionality such as sophisticated data checking, automated data conversion, and real-time estimations of haplotype simulation progress. Conclusion SimHap GUI provides a novel, easy-to-use, cross-platform solution for conducting a range of genetic and non-genetic association analyses. This provides a free alternative to commercial statistics packages that is specifically designed for genetic association analysis. PMID:19109877

SimHap GUI: an intuitive graphical user interface for genetic association analysis.

PubMed

Carter, Kim W; McCaskie, Pamela A; Palmer, Lyle J

2008-12-25

Researchers wishing to conduct genetic association analysis involving single nucleotide polymorphisms (SNPs) or haplotypes are often confronted with the lack of user-friendly graphical analysis tools, requiring sophisticated statistical and informatics expertise to perform relatively straightforward tasks. Tools, such as the SimHap package for the R statistics language, provide the necessary statistical operations to conduct sophisticated genetic analysis, but lacks a graphical user interface that allows anyone but a professional statistician to effectively utilise the tool. We have developed SimHap GUI, a cross-platform integrated graphical analysis tool for conducting epidemiological, single SNP and haplotype-based association analysis. SimHap GUI features a novel workflow interface that guides the user through each logical step of the analysis process, making it accessible to both novice and advanced users. This tool provides a seamless interface to the SimHap R package, while providing enhanced functionality such as sophisticated data checking, automated data conversion, and real-time estimations of haplotype simulation progress. SimHap GUI provides a novel, easy-to-use, cross-platform solution for conducting a range of genetic and non-genetic association analyses. This provides a free alternative to commercial statistics packages that is specifically designed for genetic association analysis.

[Study on the anti-NTHi infection of Hap recombinant protein in vivo].

PubMed

Li, Wan-yi; Wang, Bao-ning; Zuo, Feng-qiong; Zeng, Wei; Feng, Feng; Kuang, Yu; Jiang, Zhong-hua; Li, Ming-yuan

2010-07-01

To observe the immune effect of Hap recombinant protein on murine model of bronchopneumonia infected with NTHi, and explore the mechanism about the anti-NTHi infection. The C57BL/6 mice intranasally immunized with purified Hap recombinant protein and CT-B were challenged by NTHi encased in agar beads. The immunifaction of anti-infection was observed through encocyte counting of BALF, bacteria detection of lung and the pathologyical change of lung tissue. In the challenge with NTHi experiment, the inflammatory exudation of the infected murine and pathological change of lung tissue was relieved by combined immunization of Hap recombinant protein and CT-B, and quantity of NTHi in lung of the infected murine was reduced obviously. The Hap recombinant protein also had good ability of anti-NTHi infection in the murine model of NTHi bronchopneumonia. This study could offer the oretical and experimental basis for development of new vaccine against NTHi.

Galaxy Cluster Gas Mass Fractions From Sunyaev-Zeldovich Effect Measurements: Constraints on Omega(M)

NASA Technical Reports Server (NTRS)

Grego, Laura; Carlstrom, John E.; Reese, Erik D.; Holder, Gilbert P.; Holzapfel, William L.; Joy, Marshall K.; Mohr, Joseph J.; Patel, Sandeep

2001-01-01

Using sensitive centimeter-wave receivers mounted on the Owens Valley Radio Observatory and Berkeley-Illinois-Maryland-Association millimeter arrays, we have obtained interferometric measurements of the Sunyaev-Zeldovich(SZ) effect toward massive galaxy clusters. We use the SZ data to determine the pressure distribution of the cluster gas and, in combination with published X-ray temperatures, to infer the gas mass and total gravitational mass of 18 clusters. The gas mass fraction, f(g), is calculated for each cluster and is extrapolated to the fiducial radius r(500) using the results of numerical simulations. The mean f(g) within r(500) is 0.081(+ 0.009 / - 0.011) per h(100) (statistical uncertainty at 68% confidence level, assuming Omega(M) = 0.3, Omega(Lambda) = 0.7). We discuss possible sources of systematic errors in the mean f(sub g) measurement. We derive an upper limit for Omega(M) from this sample under the assumption that the mass composition of clusters within r(500) reflects the universal mass composition: Omega(M)h is less than or equal to Omega(B)/f(g). The gas mass fractions depend on cosmology through the angular diameter distance and the r(500) correction factors. For a flat universe (Omega(Lambda) is identical with 1 - Omega(M)) and h = 0.7, we find the measured gas mass fractions are consistent with Omega(M) is less than 0.40, at 68% confidence. Including estimates of the baryons contained in galaxies and the baryons which failed to become bound during the cluster formation process, we find Omega(M) is approximately equal to 0.25.

Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum.

PubMed

Castel, Virginia; Zivanovic, Svetlana; Jurat-Fuentes, Juan L; Santiago, Liliana G; Rubiolo, Amelia C; Carrara, Carlos R; Harte, Federico M

2016-10-01

Brea gum (BG) is an exudate from the Cercidium praecox tree that grows in semi-arid regions of Argentina. Some previous studies on BG have shown physicochemical characteristics and functional features similar to those of gum arabic. However, there is a need to elucidate the molecular structure of BG to understand the functionality. In this sense, BG was fractionated using hydrophobic interaction chromatography and the obtained fractions were analyzed by size exclusion chromatography. Analysis of the fractions showed that the bulk of the gum (approx. 84% of the polysaccharides) was a polysaccharide of 2.79 × 10(3)  kDa. The second major fraction (approx. 16% of the polysaccharides) was a polysaccharide-protein complex with a molecular mass of 1.92 × 10(5)  kDa. A third fraction consisted of protein species with a wide range of molecular weights. The molecular weight distribution of the protein fraction was analyzed by size exclusion chromatography. Comparison of the elution profiles of the exudates in native and reducing conditions revealed that some of the proteins were forming aggregates through disulfide bridges in native conditions. Further analysis of the protein fraction by SDS-PAGE showed proteins with molecular weight ranging from 6.5 to 66 kDa. The findings showed that BG consists of several fractions with heterogeneous chemical composition and polydisperse molecular weight distributions. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens

PubMed Central

Valansi, Clari; Moi, David; Leikina, Evgenia; Matveev, Elena; Chernomordik, Leonid V.

2017-01-01

Cell–cell fusion is inherent to sexual reproduction. Loss of HAPLESS 2/GENERATIVE CELL SPECIFIC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell–cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus–cell fusion. We demonstrate that the Caenorhabditis elegans Epithelial Fusion Failure 1 (EFF-1) somatic cell fusogen can replace HAP2/GCS1 in one of the fusing membranes, indicating that HAP2/GCS1 and EFF-1 share a similar fusion mechanism. Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and viral class II fusion proteins (e.g., Zika virus). We name this superfamily Fusexins: fusion proteins essential for sexual reproduction and exoplasmic merger of plasma membranes. We suggest a common origin and evolution of sexual reproduction, enveloped virus entry into cells, and somatic cell fusion. PMID:28137780

The Influence of Oscillatory Fractions on Mass Transfer of Non-Newtonian Fluid in Wavy-Walled Tubes for Pulsatile Flow

NASA Astrophysics Data System (ADS)

Zhu, Donghui; Bian, Yongning

2018-03-01

The shape of pipeline structure, fluid medium and flow state have important influence on the heat transfer and mass effect of fluid. In this paper, we investigated the mass transfer behavior of Non-Newtonian fluid CMC solution with 700ppm concentration in five different-sized axisymmetric wave-walled tubes for pulsatile flow. It is revealed that the effect of mass transfer is enhanced with the increase of oscillatory fractions P based on the PIV measurements. Besides, mass transfer rate was measured by the electrochemical method in the larger oscillatory points rate range. It is observed that mass transfer rate increases with the increase in P and reached the maximum mass transfer rate at the most optimal oscillatory fractions P opt. After reaching the optimal oscillatory fractions P opt, the mass transfer rate decreases with increasing P.

Final report of CCQM-K130 nitrogen mass fraction measurements in glycine

NASA Astrophysics Data System (ADS)

Medvedevskikh, Maria; Krasheninina, Maria; do Rego, Eliane C. P.; Wollinger, Wagner; Monteiro, Tânia M.; de Carvalho, Lucas J.; Acco Garcia, Steve Ali; Haraldsson, Conny; Rodriguez, M. Alejandra; Rodriguez, Gabriela; Salvo, Karino; Gavrilkin, Vladimir; Kulyk, Sergij; Samuel, Laly

2017-01-01

CCQM key comparison K-130 in the field of nitrogen mass fracton has been performed by the Inorganic Analysis Working Group (IAWG) of the Consultative Committee for Amount of Substance (CCQM). The aim of this key comparison CCQM-K130 is to support National Measurement Institutes (NMIs) and Designated Institutes (DIs) to demonstrate the validity of the procedures the employed for determination of nitrogen mass fraction in glycine. Mass fraction of nitrogen is very important pointer because the results of these measurements are often used for determination of protein mass fraction that is an important indicator of the quality of the vast majority of food products and raw materials, in particular dry milk powder. Proteins-enzymes catalyze chemical reactions, protein along with fats and carbohydrates is one of the indicators characterizing the energy value of food, so its definition is mandatory for all food products. The study material for this key comparison has been selected to be representative as one of the aminoacid - the simplest part of the protein. Glycine is an amino acid, single acid that does not have any isomers (melting point -290 °C specific heat of evaporation - 528,6 J/kg; specific melting heat - 981,1 J/kg; pKa - 2, 34, molar mass - 75,07 g/mol, density - 1,607 g/cm3). Ural Scientific Research Institute for Metrology (UNIIM) acted as the coordinating laboratory of this comparison. Nine NMIs participated in this key comparison and one NMI participated in Pilot study that was condacted in parallel. Report A contains the results of key comparison and pilot study. The results of Pilot study were excluded from the Report B Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM

BisGMA/TEGDMA dental nanocomposites containing glyoxylic acid modified high-aspect ratio hydroxyapatite nanofibers with enhanced dispersion

PubMed Central

Chen, Liang; Xu, Changqi; Wang, Yong; Shi, Jian; Yu, Qingsong

2012-01-01

The purpose of this research was to investigate the influence of the glyoxylic acid (GA) modification of hydroxyapatite (HAP) nanofibers on their dispersion in bisphenol A glycidyl methacrylate (BisGMA)/triethylene glycol dimethacrylate (TEGDMA) dental composites and also investigate the mechanical properties, water absorption, and water solubility of the resulting dental resins and composites. Scanning/Transmission electron microscopy (STEM) images showed that microsized HAP nanofiber bundles could be effectively broken down to individual HAP nanofibers with an average length of ~15 μm after the surface modification process. Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and thermal gravimetric analysis (TGA) characterization confirmed glyoxylic acid was chemically grafted on the HAP nanofiber surface, hypothetically by reacting with the amine group on HAP nanofiber surface. The enhanced dispersion of HAP nanofibers in dental matrix led to increased biaxial flexural strength (BFS) compared with the corresponding dental resins and composites filled with untreated HAP nanofibers. In addition, impregnation of small mass fractions of the glyoxylic acid modified HAP nanofibers into the BisGMA/TEGDMA dental resins (5wt%, 10wt%) or composites (2wt%, 3wt%) could also substantially improve the BFS in comparison with the controls(pure resins or dental composites filled with silica particles alone). Larger mass fractions could not further increase the mechanical property or even degrade the BFS values. Water behavior testing results indicated that the addition of glyoxylic acid modified HAP nanofibers resulted in higher water absorption and water solubility values which is not preferred for clinical application. In summary, well dispersed HAP nanofibers and their dental composites with enhanced mechanical property have been successfully fabricated but the water absorption and water solubility of such dental composites need to be

Ab initio simulation of elastic and mechanical properties of Zn- and Mg-doped hydroxyapatite (HAP).

PubMed

Aryal, Sitaram; Matsunaga, Katsuyuki; Ching, Wai-Yim

2015-07-01

Hydroxyapatite (HAP) is an important bioceramic which constitutes the mineral components of bones and hard tissues in mammals. It is bioactive and used as bioceramic coatings for metallic implants and bone fillers. HAP readily absorbs a large amount of impurities. Knowledge on the elastic and mechanical properties of impurity-doped HAP is a subject of great importance to its potential for biomedical applications. Zn and Mg are the most common divalent cations HAP absorbs. Using density function theory based ab initio methods, we have carried out a large number of ab initio calculations to obtain the bulk elastic and mechanical properties of HAP with Zn or Mg doped in different concentration at the Ca1 and Ca2 sites using large 352-atom supercells. Detailed information on their dependece on the concetraion of the substitued impurity is obtained. Our results show that Mg enhances overall elastic and bulk mechanical properties whereas Zn tends to degrade except at low concentrations. At a higher concentration, the mechanical properties of Zn and Mg doped HAP also depend significantly on impurity distribution between the Ca1 and Ca2 sites. There is a strong evidence that Zn prefers Ca2 site for substituion whereas Mg has no such preference. These results imply that proper control of dopant concentration and their site preference must carefully considered in using doped HAP for specific biomedical applications. Copyright © 2015 Elsevier Ltd. All rights reserved.

Evaluation of the Mg doping approach for Si mass fractionation correction on Nu Instruments MC-ICP Mass Spectrometers

NASA Astrophysics Data System (ADS)

Zhao, Ye; Hsieh, Yu-Te; Belshaw, Nick

2015-04-01

Silicon (Si) stable isotopes have been used in a broad range of geochemical and cosmochemical applications. A precise and accurate determination of Si isotopes is desirable to distinguish their small natural variations (< 0.2‰) in many of these studies. In the past decade, the advent of the MC-ICP-MS has spurred a remarkable improvement in the precision and accuracy of Si isotopic analysis. The instrumental mass fractionation correction is one crucial aspect of the analysis of Si isotopes. Two options are currently available: the sample-standard bracketing approach and the Mg doping approach. However, there has been a debate over the validity of the Mg doping approach. Some studies (Cardinal et al., 2003; Engström et al., 2006) favoured it compared to the sample-standard bracketing approach, whereas some other studies (e.g. De La Rocha, 2002) considered it unsuitable. This study investigates the Mg doping approach on both the Nu Plasma II and the Nu Plasma 1700. Experiments were performed in both the wet plasma and the dry plasma modes, using a number of different combinations of cones. A range of different Mg to Si ratios as well as different matrices have been used in the experiments. A sample-standard bracketing approach has also been adopted for the Si mass fractionation correction to compare with the Mg doping approach. Through assessing the mass fractionation behaviours of both Si and Mg under different instrument settings, this study aims to identity the factors which may affect the Mg doping approach and answer some key questions to the debate.

Iron-Isotopic Fractionation Studies Using Multiple Collector Inductively Coupled Plasma Mass Spectrometry

NASA Technical Reports Server (NTRS)

Anbar, A. D.; Zhang, C.; Barling, J.; Roe, J. E.; Nealson, K. H.

1999-01-01

The importance of Fe biogeochemistry has stimulated interest in Fe isotope fractionation. Recent studies using thermal ionization mass spectrometry (TIMS) and a "double spike" demonstrate the existence of biogenic Fe isotope effects. Here, we assess the utility of multiple-collector inductively-coupled plasma mass spectrometry(MC-ICP-MS) with a desolvating sample introduction system for Fe isotope studies, and present data on Fe biominerals produced by a thermophilic bacterium. Additional information is contained in the original extended abstract.

The Janus transcription factor HapX controls fungal adaptation to both iron starvation and iron excess

PubMed Central

Gsaller, Fabio; Hortschansky, Peter; Beattie, Sarah R; Klammer, Veronika; Tuppatsch, Katja; Lechner, Beatrix E; Rietzschel, Nicole; Werner, Ernst R; Vogan, Aaron A; Chung, Dawoon; Mühlenhoff, Ulrich; Kato, Masashi; Cramer, Robert A; Brakhage, Axel A; Haas, Hubertus

2014-01-01

Balance of physiological levels of iron is essential for every organism. In Aspergillus fumigatus and other fungal pathogens, the transcription factor HapX mediates adaptation to iron limitation and consequently virulence by repressing iron consumption and activating iron uptake. Here, we demonstrate that HapX is also essential for iron resistance via activating vacuolar iron storage. We identified HapX protein domains that are essential for HapX functions during either iron starvation or high-iron conditions. The evolutionary conservation of these domains indicates their wide-spread role in iron sensing. We further demonstrate that a HapX homodimer and the CCAAT-binding complex (CBC) cooperatively bind an evolutionary conserved DNA motif in a target promoter. The latter reveals the mode of discrimination between general CBC and specific HapX/CBC target genes. Collectively, our study uncovers a novel regulatory mechanism mediating both iron resistance and adaptation to iron starvation by the same transcription factor complex with activating and repressing functions depending on ambient iron availability. PMID:25092765

40 CFR 63.5340 - How do I determine the allowable HAP loss?

Code of Federal Regulations, 2013 CFR

2013-07-01

... select the appropriate HAP emission limit, expressed in pounds of HAP loss per 1,000 square feet of... months. Next, determine the annual total of leather processed in 1,000's of square feet for each product... Total of Leather Processed = 1,000's of square feet of leather processed in the previous 12 months in...

40 CFR 63.5340 - How do I determine the allowable HAP loss?

Code of Federal Regulations, 2014 CFR

2014-07-01

... select the appropriate HAP emission limit, expressed in pounds of HAP loss per 1,000 square feet of... months. Next, determine the annual total of leather processed in 1,000's of square feet for each product... Total of Leather Processed = 1,000's of square feet of leather processed in the previous 12 months in...

A Gas Chromatographic Continuous Emissions Monitoring System for the Determination of VOCs and HAPs.

PubMed

Coleman, William M; Gordon, Bert M

1996-01-01

This article describes a new gas chromatography-based emissions monitoring system for measuring volatile organic compounds (VOCs) and hazardous air pollutants (HAPs). The system is composed of a dual-column gas chromatograph equipped with thermal conductivity detectors, in which separation is optimized for fast chromatography. The system has the necessary valving for stream selection, which allows automatic calibration of the system at predetermined times and successive measurement of individual VOCs before and after a control device. Nine different VOCs (two of which are HAPs), plus methane (CH4) and carbon dioxide (CO2) are separated and quantified every two minutes. The accuracy and precision of this system has been demonstrated to be greater than 95%. The system employs a mass flow measurement device and also calculates and displays processed emission data, such as control device efficiency and total weight emitted during given time periods. Two such systems have been operational for one year in two separate gravure printing facilities; minimal upkeep is required, about one hour per month. One of these systems, used before and after a carbon adsorber, has been approved by the pertinent local permitting authority.

Inductively coupled plasma-mass spectrometry as an element-specific detector for field-flow fractionation particle separation

USGS Publications Warehouse

Taylor, Howard E.; Garbarino, John R.; Murphy, Deirdre M.; Beckett, Ronald

1992-01-01

An inductively coupled plasma-mass spectrometer was used for the quantitative measurement of trace elements In specific,submicrometer size-fraction particulates, separated by sedimentation field-flow fractionation. Fractions were collected from the eluent of the field-flow fractionation centrifuge and nebulized, with a Babington-type pneumatic nebulizer, into an argon inductively coupled plasma-mass spectrometer. Measured Ion currents were used to quantify the major, minor, and trace element composition of the size-separated colloidal (< 1-microm diameter) particulates. The composition of surface-water suspended matter collected from the Yarra and Darling rivers in Australia is presented to illustrate the usefulness of this tool for characterizing environmental materials. An adsorption experiment was performed using cadmium lon to demonstrate the utility for studying the processes of trace metal-suspended sediment interactions and contaminant transport in natural aquatic systems.

Electrospun PVA/HAp nanocomposite nanofibers: biomimetics of mineralized hard tissues at a lower level of complexity.

PubMed

Kim, Gyeong-Man; Asran, Ashraf Sh; Michler, Georg H; Simon, Paul; Kim, Jeong-Sook

2008-12-01

Based on the biomimetic approaches the present work describes a straightforward technique to mimic not only the architecture (the morphology) but also the chemistry (the composition) of the lowest level of the hierarchical organization of bone. This technique uses an electrospinning (ES) process with polyvinyl alcohol (PVA) and hydroxyapatite (HAp) nanoparticles. To determine morphology, crystalline structures and thermal properties of the resulting electrospun fibers with the pure PVA and PVA/HAp nanocomposite (NC) before electrospinning various techniques were employed, including transmission electron microscopy (TEM), high-resolution TEM (HR-TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). In addition, FT-IR spectroscopy was carried out to analyze the complex structural changes upon undergoing electrospinning as well as interactions between HAp and PVA. The morphological and crystallographic investigations revealed that the rod-like HAp nanoparticles exhibit a nanoporous morphology and are embedded within the electrospun fibers. A large number of HAp nanorods are preferentially oriented parallel to the longitudinal direction of the electrospun PVA fibers, which closely resemble the naturally mineralized hard tissues of bones. Due to abundant OH groups present in PVA and HAp nanorods, they strongly interact via hydrogen bonding within the electrospun PVA/HAp NC fibers, which results in improved thermal properties. The unique physiochemical features of the electrospun PVA/HAp NC nanofibers prepared by the ES process will open up a wide variety of future applications related to hard tissue replacement and regeneration (bone and dentin), not limited to coating implants.

Isotopic fractionation studies of uranium and plutonium using porous ion emitters as thermal ionization mass spectrometry sources

DOE PAGES

Baruzzini, Matthew L.; Hall, Howard L.; Spencer, Khalil J.; ...

2018-04-22

Investigations of the isotope fractionation behaviors of plutonium and uranium reference standards were conducted employing platinum and rhenium (Pt/Re) porous ion emitter (PIE) sources, a relatively new thermal ionization mass spectrometry (TIMS) ion source strategy. The suitability of commonly employed, empirically developed mass bias correction laws (i.e., the Linear, Power, and Russell's laws) for correcting such isotope ratio data was also determined. Corrected plutonium isotope ratio data, regardless of mass bias correction strategy, were statistically identical to that of the certificate, however, the process of isotope fractionation behavior of plutonium using the adopted experimental conditions was determined to be bestmore » described by the Power law. Finally, the fractionation behavior of uranium, using the analytical conditions described herein, is also most suitably modeled using the Power law, though Russell's and the Linear law for mass bias correction rendered results that were identical, within uncertainty, to the certificate value.« less

Isotopic fractionation studies of uranium and plutonium using porous ion emitters as thermal ionization mass spectrometry sources

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baruzzini, Matthew L.; Hall, Howard L.; Spencer, Khalil J.

Investigations of the isotope fractionation behaviors of plutonium and uranium reference standards were conducted employing platinum and rhenium (Pt/Re) porous ion emitter (PIE) sources, a relatively new thermal ionization mass spectrometry (TIMS) ion source strategy. The suitability of commonly employed, empirically developed mass bias correction laws (i.e., the Linear, Power, and Russell's laws) for correcting such isotope ratio data was also determined. Corrected plutonium isotope ratio data, regardless of mass bias correction strategy, were statistically identical to that of the certificate, however, the process of isotope fractionation behavior of plutonium using the adopted experimental conditions was determined to be bestmore » described by the Power law. Finally, the fractionation behavior of uranium, using the analytical conditions described herein, is also most suitably modeled using the Power law, though Russell's and the Linear law for mass bias correction rendered results that were identical, within uncertainty, to the certificate value.« less

Mass fraction assignment of folic acid in a high purity material

NASA Astrophysics Data System (ADS)

Westwood, Steven; Josephs, Ralf; Choteau, Tiphaine; Daireaux, Adeline; Stoppacher, Norbert; Wielgosz, Robert; Davies, Stephen; de Rego, Eliane; Wollinger, Wagner; Garrido, Bruno; Fernandes, Jane; Lima, Jonathan; Oliveira, Rodrigo; de Sena, Rodrigo; Windust, Anthony; Huang, Ting; Dai, Xinhua; Quan, Can; He, Haihong; Zhang, Wei; Wei, Chao; Li, Na; Gao, Dexin; Liu, Zhao; Lo, Man-fung; Wong, Wai-fun; Pfeifer, Dietmar; Koch, Matthias; Dorgerloh, Ute; Rothe, Robert; Philip, Rosemary; Hirari, Nobuyasu; Fazlin Rezali, Mohd; Salazar Arzate, Claudia Marcela; Pedraza Evelina Berenice, Mercado; Serrano Caballero, Victor; Arce Osuna, Mariana; Krylov, A.; Kharitonov, S.; Lopushanskaya, E.; Liu, Qinde; Tang Lin, Teo; Fernandes-Whaley, Maria; Quinn, Laura; Nhlapo, Nontete; Prevoo-Franzsen, Desiree; Archer, Marcelle; Kim, Byungjoo; Baek, Song-Yee; Lee, Sunyoung; Lee, Joonhee; Marbumrung, Sornkrit; Kankaew, Ponhatai; Chaorenpornpukdee, Kanokrat; Chaipet, Thitiphan; Shearman, Kittiya; Ceyhan Goren, Ahmet; Gunduz, Simay; Yilmaz, Hasibe; Un, Ilker; Bilsel, Gokhan; Clarkson, Cailean; Bedner, Mary; Camara, Johanna E.; Lang, Brian E.; Lippa, Katrice A.; Nelson, Michael A.; Toman, Blaza; Yu, Lee L.

2018-01-01

The comparison required the assignment of the mass fraction of folic acid present as the main component in the comparison sample. Performance in the comparison is representative of a laboratory's measurement capability for the purity assignment of organic compounds of medium structural complexity [molecular weight range 300–500] and high polarity (pKOW < ‑2). Methods used by the eighteen participating NMIs or DIs were based on a mass balance (summation of impurities) or qNMR approach, or the combination of data obtained using both methods. The qNMR results tended to give slightly lower values for the content of folic acid, albeit with larger associated uncertainties, compared with the results obtained by mass balance procedures. Possible reasons for this divergence are discussed in the report, without reaching a definitive conclusion as to their origin. The comparison demonstrates that for a structurally complex polar organic compound containing a high water content and presenting a number of additional analytical challenges, the assignment of the mass fraction content property value of the main component can reasonably be achieved with an associated relative standard uncertainty in the assigned value of 0.5% Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

Fractionated Mercury Isotopes in Fish: The Effects of Nuclear Mass, Spin, and Volume

NASA Astrophysics Data System (ADS)

Das, R.; Odom, A. L.

2007-12-01

Mercury is long known as a common environmental contaminant. In methylated form it is even more toxic and the methylation process is facilitated by microbial activities. Methyl mercury easily crosses cell membrane and accumulates in soft tissues of fishes and finally biomagnifies with increasing trophic levels. Natural variations in the isotopic composition of mercury have been reported and such variations have emphasized mass dependent fractionations, while theory and laboratory experiments indicate that mass-independent isotopic fractionation (MIF) effects are likely to be found as well. This study focuses on the MIF of mercury isotopes in the soft tissues of fishes. Samples include both fresh water and marine fish, from different continents and oceans. Approximately 1 gm of fish soft tissue was dissolved in 5 ml of conc. aqua regia for 24 hrs and filtered through a ¬¬¬100 μm filter paper and diluted with DI water. Hg is measured as a gaseous phase generated by reduction of the sample with SnCl2 in a continuous- flow cold-vapor generator connected to a Thermo-Finnigan Neptune MC-ICPMS. To minimize instrumental fractionation isotope ratios were measured by sample standard bracketing and reported as δ‰ relative to NIST SRM 3133 Hg standard where δAHg = [(A Hg/202Hg)sample/(A Hg/202Hg)NIST313] -1 ×1000‰. In this study we have measured the isotope ratios 198Hg/202Hg, 199Hg/202Hg, 200Hg/202Hg, 201Hg/202Hg and 204Hg/202Hg. In all the fish samples δ198Hg, δ200Hg, δ202Hg, δ204Hg define a mass- dependent fractionation sequence, where as the δ199Hg and δ201Hg depart from the mass- dependent fractionation line and indicate an excess of the odd-N isotopes. The magnitude of the deviation (ΔAHg where A=199 or 201) as obtained by difference between the measured δ199Hg and δ201Hg of the samples and the value obtained by linear scaling defined by the even-N isotopes ranges from approximately 0.2 ‰ to 3‰. The ratios of Δ199Hg /Δ201Hg range from 0.8 to 1

Pollution prevention and the use of low-VOC/HAP coatings at wood furniture manufacturing facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marshall, A.M.; Spaight, J.L.; Jones, J.W.

1999-07-01

Midwest Research Institute, under a cooperative agreement with the Air Pollution Prevention and Control Division of the U.S. Environmental Protection Agency's (EPA's) National Risk Management Research Laboratory, is conducting a study to identify wood furniture and cabinet manufacturing facilities that have converted to low-volatile organic compound/hazardous air pollutant (VOC/HAP) coatings and to develop case studies for those facilities. The case studies include: (1) a discussion of the types of products each facility manufactures; (2) the types of low-VOC/HAP coatings each facility is using; (3) problems encountered in converting to low-VOC/HAP coatings; (4) equipment changes that were required; (5) the costsmore » associated with the conversion process, including capital costs associated with equipment purchases, research and development costs, and operating costs such as operator training in new application techniques; (6) advantages/disadvantages of the low-VOC/HAP coatings; and (7) customer feedback on products finished with the low-VOC/HAP coatings. The primary goals of the project are (1) to demonstrate that low-VOC/HAP coatings can be used successfully by many wood furniture manufacturing facilities, and (2) to assist other wood furniture manufacturing facilities in their conversion to low-VOC/HAP coatings, in particular facilities that do not have the resources to devote to extensive coatings research. This paper discusses the progress of the project and pollution prevention options at wood furniture manufacturing facilities and the regulatory requirements (e.g., the National Emissions Standards for Hazardous Air Pollutants [NESHAP] for Wood Furniture Manufacturing Operations) that these facilities face.« less

Surviving Mass Extinctions through Biomineralized DNA.

PubMed

Turon, Pau; Puiggalí, Jordi; Bertrán, Oscar; Alemán, Carlos

2015-12-21

Even in the worst of conditions, such as those which occurred during mass extinction events, life on Earth never totally stopped. Aggressive chemical and physical attacks able to sterilize or poison living organisms occurred repeatedly. Surprisingly, DNA was not degraded, denatured or modified to the point of losing the capability of transferring the genetic information to the next generations. After the events of mass extinction life was able to survive and thrive. DNA was passed on despite being an extremely fragile biomolecule. The potential implications of hydroxyapatite protection of DNA are discussed in this Concept article including how DNA acts as a template for hydroxyapatite (HAp) formation, how cell death can trigger biomineralization, and how DNA can be successfully released from HAp when the conditions are favorable for life. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Evaluation of model-predicted hazardous air pollutants (HAPs) near a mid-sized U.S. airport

NASA Astrophysics Data System (ADS)

Vennam, Lakshmi Pradeepa; Vizuete, William; Arunachalam, Saravanan

2015-10-01

Accurate modeling of aircraft-emitted pollutants in the vicinity of airports is essential to study the impact on local air quality and to answer policy and health-impact related issues. To quantify air quality impacts of airport-related hazardous air pollutants (HAPs), we carried out a fine-scale (4 × 4 km horizontal resolution) Community Multiscale Air Quality model (CMAQ) model simulation at the T.F. Green airport in Providence (PVD), Rhode Island. We considered temporally and spatially resolved aircraft emissions from the new Aviation Environmental Design Tool (AEDT). These model predictions were then evaluated with observations from a field campaign focused on assessing HAPs near the PVD airport. The annual normalized mean error (NME) was in the range of 36-70% normalized mean error for all HAPs except for acrolein (>70%). The addition of highly resolved aircraft emissions showed only marginally incremental improvements in performance (1-2% decrease in NME) of some HAPs (formaldehyde, xylene). When compared to a coarser 36 × 36 km grid resolution, the 4 × 4 km grid resolution did improve performance by up to 5-20% NME for formaldehyde and acetaldehyde. The change in power setting (from traditional International Civil Aviation Organization (ICAO) 7% to observation studies based 4%) doubled the aircraft idling emissions of HAPs, but led to only a 2% decrease in NME. Overall modeled aircraft-attributable contributions are in the range of 0.5-28% near a mid-sized airport grid-cell with maximum impacts seen only within 4-16 km from the airport grid-cell. Comparison of CMAQ predictions with HAP estimates from EPA's National Air Toxics Assessment (NATA) did show similar annual mean concentrations and equally poor performance. Current estimates of HAPs for PVD are a challenge for modeling systems and refinements in our ability to simulate aircraft emissions have made only incremental improvements. Even with unrealistic increases in HAPs aviation emissions the model

Postnatal Loss of Hap1 Reduces Hippocampal Neurogenesis and Causes Adult Depressive-Like Behavior in Mice

PubMed Central

Xiang, Jianxing; Yan, Sen; Li, Shi-Hua; Li, Xiao-Jiang

2015-01-01

Depression is a serious mental disorder that affects a person’s mood, thoughts, behavior, physical health, and life in general. Despite our continuous efforts to understand the disease, the etiology of depressive behavior remains perplexing. Recently, aberrant early life or postnatal neurogenesis has been linked to adult depressive behavior; however, genetic evidence for this is still lacking. Here we genetically depleted the expression of huntingtin-associated protein 1 (Hap1) in mice at various ages or in selective brain regions. Depletion of Hap1 in the early postnatal period, but not later life, led to a depressive-like phenotype when the mice reached adulthood. Deletion of Hap1 in adult mice rendered the mice more susceptible to stress-induced depressive-like behavior. Furthermore, early Hap1 depletion impaired postnatal neurogenesis in the dentate gyrus (DG) of the hippocampus and reduced the level of c-kit, a protein expressed in neuroproliferative zones of the rodent brain and that is stabilized by Hap1. Importantly, stereotaxically injected adeno-associated virus (AAV) that directs the expression of c-kit in the hippocampus promoted postnatal hippocampal neurogenesis and ameliorated the depressive-like phenotype in conditional Hap1 KO mice, indicating a link between postnatal-born hippocampal neurons and adult depression. Our results demonstrate critical roles for Hap1 and c-kit in postnatal neurogenesis and adult depressive behavior, and also suggest that genetic variations affecting postnatal neurogenesis may lead to adult depression. PMID:25875952

Postnatal loss of hap1 reduces hippocampal neurogenesis and causes adult depressive-like behavior in mice.

PubMed

Xiang, Jianxing; Yan, Sen; Li, Shi-Hua; Li, Xiao-Jiang

2015-04-01

Depression is a serious mental disorder that affects a person's mood, thoughts, behavior, physical health, and life in general. Despite our continuous efforts to understand the disease, the etiology of depressive behavior remains perplexing. Recently, aberrant early life or postnatal neurogenesis has been linked to adult depressive behavior; however, genetic evidence for this is still lacking. Here we genetically depleted the expression of huntingtin-associated protein 1 (Hap1) in mice at various ages or in selective brain regions. Depletion of Hap1 in the early postnatal period, but not later life, led to a depressive-like phenotype when the mice reached adulthood. Deletion of Hap1 in adult mice rendered the mice more susceptible to stress-induced depressive-like behavior. Furthermore, early Hap1 depletion impaired postnatal neurogenesis in the dentate gyrus (DG) of the hippocampus and reduced the level of c-kit, a protein expressed in neuroproliferative zones of the rodent brain and that is stabilized by Hap1. Importantly, stereotaxically injected adeno-associated virus (AAV) that directs the expression of c-kit in the hippocampus promoted postnatal hippocampal neurogenesis and ameliorated the depressive-like phenotype in conditional Hap1 KO mice, indicating a link between postnatal-born hippocampal neurons and adult depression. Our results demonstrate critical roles for Hap1 and c-kit in postnatal neurogenesis and adult depressive behavior, and also suggest that genetic variations affecting postnatal neurogenesis may lead to adult depression.

40 CFR Table 4 to Subpart Vvvvvv... - Emission Limits and Compliance Requirements for Metal HAP Process Vents

Code of Federal Regulations, 2010 CFR

2010-07-01

... Requirements for Metal HAP Process Vents 4 Table 4 to Subpart VVVVVV of Part 63 Protection of Environment... of Part 63—Emission Limits and Compliance Requirements for Metal HAP Process Vents As required in § 63.11496(f), you must comply with the requirements for metal HAP process vents as shown in the...

Parallel nano-assembling of a multifunctional GO/HapNP coating on ultrahigh-purity magnesium for biodegradable implants

NASA Astrophysics Data System (ADS)

Santos, C.; Piedade, C.; Uggowitzer, P. J.; Montemor, M. F.; Carmezim, M. J.

2015-08-01

This work reports the one-step fabrication of a novel coating on ultra high purity magnesium using a parallel nano assembling process. The multifunctional biodegradable surface was obtained by adding hydroxyapatite nanoparticles (HapNP) plus graphene oxide (GO). The coating was characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffractometer (XRD), micro-Raman spectroscopy. The thin phosphate coating (thickness of 1 μm) reveals a uniform coverage with cypress like structures. The incorporation of HapNP and GO promotes the hydrophilic behavior of the coating surface. The results revealed that the proposed coating can be used to tailor the surface properties such as wettability by adjusting the contents of HapNP and GO. The in vitro degradation rate of the coated magnesium suggests that the presence of HapNP and GO/HapNP in the phosphate coating decreased the current density compared to the single phosphate coating and uncoated magnesium. This study also reveals the HapNP/GO/phosphate coating induces apatite formation, showing suitable degradability that makes it a promising coating candidate for enhanced bone regeneration.

ptr-MIR169 is a posttranscriptional repressor of PtrHAP2 during vegetative bud dormancy period of aspen (Populus tremuloides) trees

DOE Office of Scientific and Technical Information (OSTI.GOV)

Potkar, Rewati; Recla, Jill; Busov, Victor, E-mail: vbusov@mtu.edu

2013-02-15

Highlights: ► We show a novel microRNA-mediated mechanism for control of bud dormancy in trees. ► ptr-MIR169a and PtrHAP2–5 gene showed inverse expression during dormancy period. ► The PtrHAP2–5 decline in abundance correlated with high ptr-MIR169a levels. ► PtrHAP2–5 cleavage occurred at the miR169 site during PtrHAP2–5 transcript decline. ► Our results show that miR169 attenuates PtrHAP2–5 transcript during dormancy. -- Abstract: Dormancy is a mechanism evolved in woody perennial plants to survive the winter freezing and dehydration stress via temporary suspension of growth. We have identified two aspen microRNAs (ptr-MIR169a and ptr-MIR169h) which were highly and specifically expressed inmore » dormant floral and vegetative buds. ptr-MIR169a and its target gene PtrHAP2–5 showed inverse expression patterns during the dormancy period. ptr-MIR169a transcript steadily increased through the first half of the dormancy period and gradually declined with the approach of active growing season. PtrHAP2–5 abundance was higher in the beginning of the dormancy period but rapidly declined thereafter. The decline of PtrHAP2–5 correlated with the high levels of ptr-MIR169a accumulation, suggesting miR169-mediated attenuation of the target PtrHAP2–5 transcript. We experimentally verified the cleavage of PtrHAP2–5 at the predicted miR169a site at the time when PtrHAP2–5 transcript decline was observed. HAP2 is a subunit of a nuclear transcription factor Y (NF-Y) complex consisting of two other units, HAP3 and HAP5. Using digital expression profiling we show that poplar HAP2 and HAP5 are preferentially detected in dormant tissues. Our study shows that microRNAs play a significant and as of yet unknown and unstudied role in regulating the timing of bud dormancy in trees.« less

A biocompatible and novelly-defined Al-HAP adsorption membrane for highly effective removal of fluoride from drinking water.

PubMed

He, Junyong; Chen, Kai; Cai, Xingguo; Li, Yulian; Wang, Chengming; Zhang, Kaisheng; Jin, Zhen; Meng, Fanli; Wang, Xuguang; Kong, Lingtao; Liu, Jinhuai

2017-03-15

A biocompatible and novelly-defined adsorption membrane for rapid removal of fluoride was prepared. Both adsorption and membrane techniques were used in this research. Al(OH) 3 nanoparticles modified hydroxyapatite (Al-HAP) nanowires were developed and made into Al-HAP membrane. The adsorption data of Al-HAP adsorbent could be well described by Freundlich isotherm model while the adsorption kinetic followed pseudo-second-order model. The maximum of adsorption capacity was 93.84mg/g when the fluoride concentration was 200mg/L. The adsorption mechanism was anion exchanges and electrostatic interactions. The contribution rates of HAP nanowires and Al(OH) 3 nanoparticles in fluoride removal were 36.70% and 63.30%, respectively. The fixed-bed column test demonstrate that the Al-HAP was biocompatible and in a good stability during the process of water treatment. The fluoride removal abilities of Al-HAP membrane with 0.3mm thickness could reach 1568L/m 2 when fluoride concentrations were 5mg/L. This study indicated that the Al-HAP membrane could be developed into a very viable technology for highly effective removal of fluoride from drinking water. Copyright © 2016 Elsevier Inc. All rights reserved.

DDA Computations of Porous Aggregates with Forsterite Crystals: Effects of Crystal Shape and Crystal Mass Fraction

NASA Technical Reports Server (NTRS)

Wooden, Diane H.; Lindsay, Sean S.; Harker, David; Woodward, Charles; Kelley, Michael S.; Kolokolova, Ludmilla

2015-01-01

Porous aggregate grains are commonly found in cometary dust samples and are needed to model cometary IR spectral energy distributions (SEDs). Models for thermal emissions from comets require two forms of silicates: amorphous and crystalline. The dominant crystal resonances observed in comet SEDs are from Forsterite (Mg2SiO4). The mass fractions that are crystalline span a large range from 0.0 < or = fcrystal < or = 0.74. Radial transport models that predict the enrichment of the outer disk (>25 AU at 1E6 yr) by inner disk materials (crystals) are challenged to yield the highend-range of cometary crystal mass fractions. However, in current thermal models, Forsterite crystals are not incorporated into larger aggregate grains but instead only are considered as discrete crystals. A complicating factor is that Forsterite crystals with rectangular shapes better fit the observed spectral resonances in wavelength (11.0-11.15 microns, 16, 19, 23.5, 27, and 33 microns), feature asymmetry and relative height (Lindley et al. 2013) than spherically or elliptically shaped crystals. We present DDA-DDSCAT computations of IR absorptivities (Qabs) of 3 micron-radii porous aggregates with 0.13 < or = fcrystal < or = 0.35 and with polyhedral-shaped Forsterite crystals. We can produce crystal resonances with similar appearance to the observed resonances of comet Hale- Bopp. Also, a lower mass fraction of crystals in aggregates can produce the same spectral contrast as a higher mass fraction of discrete crystals; the 11micron and 23 micron crystalline resonances appear amplified when crystals are incorporated into aggregates composed otherwise of spherically shaped amorphous Fe-Mg olivines and pyroxenes. We show that the optical properties of a porous aggregate is not linear combination of its monomers, so aggregates need to be computed. We discuss the consequence of lowering comet crystal mass fractions by modeling IR SEDs with aggregates with crystals, and the implications for radial

Mass dependent stable isotope fractionation of mercury during mer mediated microbial degradation of monomethylmercury

NASA Astrophysics Data System (ADS)

Kritee, K.; Barkay, Tamar; Blum, Joel D.

2009-03-01

Controlling bioaccumulation of toxic monomethylmercury (MMHg) in aquatic food chains requires differentiation between biotic and abiotic pathways that lead to its production and degradation. Recent mercury (Hg) stable isotope measurements of natural samples suggest that Hg isotope ratios can be a powerful proxy for tracing dominant Hg transforming pathways in aquatic ecosystems. Specifically, it has been shown that photo-degradation of MMHg causes both mass dependent (MDF) and mass independent fractionation (MIF) of Hg isotopes. Because the extent of MDF and MIF observed in natural samples (e.g., fish, soil and sediments) can potentially be used to determine the relative importance of pathways leading to MMHg accumulation, it is important to determine the potential role of microbial pathways in contributing to the fractionation, especially MIF, observed in these samples. This study reports the extent of fractionation of Hg stable isotopes during degradation of MMHg to volatile elemental Hg and methane via the microbial Hg resistance ( mer) pathway in Escherichia coli carrying a mercury resistance ( mer) genetic system on a multi-copy plasmid. During experimental microbial degradation of MMHg, MMHg remaining in reactors became progressively heavier (increasing δ202Hg) with time and underwent mass dependent Rayleigh fractionation with a fractionation factor α202/198 = 1.0004 ± 0.0002 (2SD). However, MIF was not observed in any of the microbial MMHg degradation experiments indicating that the isotopic signature left by mer mediated MMHg degradation is significantly different from fractionation observed during DOC mediated photo-degradation of MMHg. Additionally, a clear suppression of Hg isotope fractionation, both during reduction of Hg(II) and degradation of MMHg, was observed when the cell densities increased, possibly due to a reduction in substrate bioavailability. We propose a multi-step framework for understanding the extent of fractionation seen in our MMHg

The Haemophilus influenzae Hap autotransporter mediates microcolony formation and adherence to epithelial cells and extracellular matrix via binding regions in the C-terminal end of the passenger domain.

PubMed

Fink, Doran L; Buscher, Amy Z; Green, Bruce; Fernsten, Phillip; St Geme, Joseph W

2003-03-01

The pathogenesis of non-typable Haemophilus influenzae disease begins with colonization of the nasopharynx and is facilitated by bacterial adherence to respiratory mucosa. The H. influenzae Hap autotransporter is a non-pilus adhesin that promotes adherence to epithelial cells and selected extracellular matrix proteins and mediates bacterial aggregation and microcolony formation. In addition, Hap has serine protease activity. Hap contains a 110 kDa internal passenger domain called HapS and a 45 kDa C-terminal translocator domain called Hapbeta. In the present study, we sought to define the structural basis for Hap adhesive activities. Based on experiments using a panel of monoclonal antibodies against HapS, a deletion derivative lacking most of HapS and a purified fragment of HapS, we established that adherence to epithelial cells is mediated by sequences within the C-terminal 311 residues of HapS. In additional experiments, we discovered that bacterial aggregation is also mediated by sequences within the C-terminal 311 residues of HapS and occurs via HapS-HapS interaction between molecules on neighbouring organisms. Finally, we found that adherence to fibronectin, laminin and collagen IV is mediated in part by sequences within the C-terminal 311 residues of HapS and in full by sequences within the C-terminal 511 residues of HapS. Taken together, these results demonstrate that all Hap adhesive activities reside in the C-terminal portion of HapS. Coupled with earlier observations, the current results establish that HapS adhesive activities and HapS protease activity are contained in separate modules of the protein.

interPopula: a Python API to access the HapMap Project dataset

PubMed Central

2010-01-01

Background The HapMap project is a publicly available catalogue of common genetic variants that occur in humans, currently including several million SNPs across 1115 individuals spanning 11 different populations. This important database does not provide any programmatic access to the dataset, furthermore no standard relational database interface is provided. Results interPopula is a Python API to access the HapMap dataset. interPopula provides integration facilities with both the Python ecology of software (e.g. Biopython and matplotlib) and other relevant human population datasets (e.g. Ensembl gene annotation and UCSC Known Genes). A set of guidelines and code examples to address possible inconsistencies across heterogeneous data sources is also provided. Conclusions interPopula is a straightforward and flexible Python API that facilitates the construction of scripts and applications that require access to the HapMap dataset. PMID:21210977

Comparison of HapMap and 1000 Genomes Reference Panels in a Large-Scale Genome-Wide Association Study.

PubMed

de Vries, Paul S; Sabater-Lleal, Maria; Chasman, Daniel I; Trompet, Stella; Ahluwalia, Tarunveer S; Teumer, Alexander; Kleber, Marcus E; Chen, Ming-Huei; Wang, Jie Jin; Attia, John R; Marioni, Riccardo E; Steri, Maristella; Weng, Lu-Chen; Pool, Rene; Grossmann, Vera; Brody, Jennifer A; Venturini, Cristina; Tanaka, Toshiko; Rose, Lynda M; Oldmeadow, Christopher; Mazur, Johanna; Basu, Saonli; Frånberg, Mattias; Yang, Qiong; Ligthart, Symen; Hottenga, Jouke J; Rumley, Ann; Mulas, Antonella; de Craen, Anton J M; Grotevendt, Anne; Taylor, Kent D; Delgado, Graciela E; Kifley, Annette; Lopez, Lorna M; Berentzen, Tina L; Mangino, Massimo; Bandinelli, Stefania; Morrison, Alanna C; Hamsten, Anders; Tofler, Geoffrey; de Maat, Moniek P M; Draisma, Harmen H M; Lowe, Gordon D; Zoledziewska, Magdalena; Sattar, Naveed; Lackner, Karl J; Völker, Uwe; McKnight, Barbara; Huang, Jie; Holliday, Elizabeth G; McEvoy, Mark A; Starr, John M; Hysi, Pirro G; Hernandez, Dena G; Guan, Weihua; Rivadeneira, Fernando; McArdle, Wendy L; Slagboom, P Eline; Zeller, Tanja; Psaty, Bruce M; Uitterlinden, André G; de Geus, Eco J C; Stott, David J; Binder, Harald; Hofman, Albert; Franco, Oscar H; Rotter, Jerome I; Ferrucci, Luigi; Spector, Tim D; Deary, Ian J; März, Winfried; Greinacher, Andreas; Wild, Philipp S; Cucca, Francesco; Boomsma, Dorret I; Watkins, Hugh; Tang, Weihong; Ridker, Paul M; Jukema, Jan W; Scott, Rodney J; Mitchell, Paul; Hansen, Torben; O'Donnell, Christopher J; Smith, Nicholas L; Strachan, David P; Dehghan, Abbas

2017-01-01

An increasing number of genome-wide association (GWA) studies are now using the higher resolution 1000 Genomes Project reference panel (1000G) for imputation, with the expectation that 1000G imputation will lead to the discovery of additional associated loci when compared to HapMap imputation. In order to assess the improvement of 1000G over HapMap imputation in identifying associated loci, we compared the results of GWA studies of circulating fibrinogen based on the two reference panels. Using both HapMap and 1000G imputation we performed a meta-analysis of 22 studies comprising the same 91,953 individuals. We identified six additional signals using 1000G imputation, while 29 loci were associated using both HapMap and 1000G imputation. One locus identified using HapMap imputation was not significant using 1000G imputation. The genome-wide significance threshold of 5×10-8 is based on the number of independent statistical tests using HapMap imputation, and 1000G imputation may lead to further independent tests that should be corrected for. When using a stricter Bonferroni correction for the 1000G GWA study (P-value < 2.5×10-8), the number of loci significant only using HapMap imputation increased to 4 while the number of loci significant only using 1000G decreased to 5. In conclusion, 1000G imputation enabled the identification of 20% more loci than HapMap imputation, although the advantage of 1000G imputation became less clear when a stricter Bonferroni correction was used. More generally, our results provide insights that are applicable to the implementation of other dense reference panels that are under development.

Comparison of HapMap and 1000 Genomes Reference Panels in a Large-Scale Genome-Wide Association Study

PubMed Central

de Vries, Paul S.; Sabater-Lleal, Maria; Chasman, Daniel I.; Trompet, Stella; Kleber, Marcus E.; Chen, Ming-Huei; Wang, Jie Jin; Attia, John R.; Marioni, Riccardo E.; Weng, Lu-Chen; Grossmann, Vera; Brody, Jennifer A.; Venturini, Cristina; Tanaka, Toshiko; Rose, Lynda M.; Oldmeadow, Christopher; Mazur, Johanna; Basu, Saonli; Yang, Qiong; Ligthart, Symen; Hottenga, Jouke J.; Rumley, Ann; Mulas, Antonella; de Craen, Anton J. M.; Grotevendt, Anne; Taylor, Kent D.; Delgado, Graciela E.; Kifley, Annette; Lopez, Lorna M.; Berentzen, Tina L.; Mangino, Massimo; Bandinelli, Stefania; Morrison, Alanna C.; Hamsten, Anders; Tofler, Geoffrey; de Maat, Moniek P. M.; Draisma, Harmen H. M.; Lowe, Gordon D.; Zoledziewska, Magdalena; Sattar, Naveed; Lackner, Karl J.; Völker, Uwe; McKnight, Barbara; Huang, Jie; Holliday, Elizabeth G.; McEvoy, Mark A.; Starr, John M.; Hysi, Pirro G.; Hernandez, Dena G.; Guan, Weihua; Rivadeneira, Fernando; McArdle, Wendy L.; Slagboom, P. Eline; Zeller, Tanja; Psaty, Bruce M.; Uitterlinden, André G.; de Geus, Eco J. C.; Stott, David J.; Binder, Harald; Hofman, Albert; Franco, Oscar H.; Rotter, Jerome I.; Ferrucci, Luigi; Spector, Tim D.; Deary, Ian J.; März, Winfried; Greinacher, Andreas; Wild, Philipp S.; Cucca, Francesco; Boomsma, Dorret I.; Watkins, Hugh; Tang, Weihong; Ridker, Paul M.; Jukema, Jan W.; Scott, Rodney J.; Mitchell, Paul; Hansen, Torben; O'Donnell, Christopher J.; Smith, Nicholas L.; Strachan, David P.

2017-01-01

An increasing number of genome-wide association (GWA) studies are now using the higher resolution 1000 Genomes Project reference panel (1000G) for imputation, with the expectation that 1000G imputation will lead to the discovery of additional associated loci when compared to HapMap imputation. In order to assess the improvement of 1000G over HapMap imputation in identifying associated loci, we compared the results of GWA studies of circulating fibrinogen based on the two reference panels. Using both HapMap and 1000G imputation we performed a meta-analysis of 22 studies comprising the same 91,953 individuals. We identified six additional signals using 1000G imputation, while 29 loci were associated using both HapMap and 1000G imputation. One locus identified using HapMap imputation was not significant using 1000G imputation. The genome-wide significance threshold of 5×10−8 is based on the number of independent statistical tests using HapMap imputation, and 1000G imputation may lead to further independent tests that should be corrected for. When using a stricter Bonferroni correction for the 1000G GWA study (P-value < 2.5×10−8), the number of loci significant only using HapMap imputation increased to 4 while the number of loci significant only using 1000G decreased to 5. In conclusion, 1000G imputation enabled the identification of 20% more loci than HapMap imputation, although the advantage of 1000G imputation became less clear when a stricter Bonferroni correction was used. More generally, our results provide insights that are applicable to the implementation of other dense reference panels that are under development. PMID:28107422

Structural insights into activation and inhibition of histo-aspartic protease (HAP) from Plasmodium falciparum

PubMed Central

Bhaumik, Prasenjit; Xiao, Huogen; Hidaka, Koushi; Gustchina, Alla; Kiso, Yoshiaki; Yada, Rickey Y.; Wlodawer, Alexander

2012-01-01

Histo-aspartic protease (HAP) from Plasmodium falciparum offers a promising target for the development of novel antimalarial drugs. HAP exhibits high sequence similarity to pepsin-like aspartic proteases, but one of the two catalytic aspartates, Asp32, is replaced by histidine. Crystal structures of the truncated zymogen of HAP and of the complex of the mature enzyme with inhibitor KNI-10395 have been determined at 2.1 and 2.5 Å resolution, respectively. As in other proplasmepsins, the propeptide of the zymogen interacts with the C-terminal domain of the enzyme, forcing the N- and C- terminal domains apart, thereby separating His32 and Asp215 and preventing formation of the mature active site. In the inhibitor complex the enzyme forms a tight domain-swapped dimer, not previously seen in any aspartic proteases. The inhibitor is found in an unprecedented conformation resembling the letter “U”, stabilized by two intramolecular hydrogen bonds. Surprisingly, the location and conformation of the inhibitor are similar to the fragment of helix 2 comprising residues 34p–38p in the prosegments of the zymogens of gastric aspartic proteases; a corresponding helix assumes a vastly different orientation in proplasmepsins. Each inhibitor molecule is in contact with two molecules of HAP, interacting with the carboxylate group of the catalytic Asp215 of one HAP protomer through a water molecule, while also making a direct hydrogen bond to Glu278A′ of the other protomer. A comparison of the shifts in the positions of the catalytic residues in the inhibitor complex presented here with those published previously gives further hints regarding the enzymatic mechanism of HAP. PMID:21928835

ESR investigation of ROS generated by H2O2 bleaching with TiO2 coated HAp.

PubMed

Saita, Makiko; Kobayashi, Kyo; Kobatashi, Kyou; Yoshino, Fumihiko; Hase, Hiriko; Nonami, Toru; Kimoto, Katsuhiko; Lee, Masaichi-Chang-il

2012-01-01

It is well known that clinical bleaching can be achieved with a solution of 30% hydrogen peroxide (H2O2) or H2O2/titanium dioxide (TiO2) combination. This study examined the hypothesis that TiO2 coated with hydroxyapatite (HAp-TiO2) can generate reactive oxygen species (ROS). ROS are generated via photocatalysis using electron spin resonance (ESR). The bleaching properties of HAp-TiO2 in the presence of H2O2 can be measured using hematoporphyrin litmus paper and extracted teeth. We demonstrate that superoxides (O2(•-)) and hydroxyl radicals (HO(•)) can be generated through excitation of anatase TiO2, rutile TiO2, anatase HAp-TiO2, and rutile HAp-TiO2 in the presence of H2O2. The combination of R HAp-TiO2 with H2O2 produced the highest level of HO(•) generation and the most marked bleaching effects of all the samples. The superior bleaching effects exhibited by R HAp-TiO2 with H2O2 suggest that this combination may lead to novel methods for the clinical application of bleaching treatments.

Hilson Adolescent Profile (HAP): Hilson Research Abstracts.

ERIC Educational Resources Information Center

Hilson Research Inc., Kew Gardens, NY.

Abstracts and bibliographic citations are given for the following documents concerned with the use and characteristics of the Hilson Adolescent Profile (HAP): (1) "Use of the Hilson Adolescent Profile To Compare Juvenile Offenders with Junior and Senior High School Students" (R. E. Inwald and K. E. Brobst); (2) "The Effectiveness of…

A new series of HAPs as anti-HBV agents targeting at capsid assembly.

PubMed

Yang, Xiu-yan; Xu, Xiao-qian; Guan, Hua; Wang, Li-li; Wu, Qin; Zhao, Guo-ming; Li, Song

2014-09-01

A series of novel Heteroaryldihydropyrimidines (HAPs) derivatives were designed and synthesized as potent inhibitors of HBV capsid assembly. These compounds were prepared from efforts to optimize an earlier series of HAPs, and compounds Mo1, Mo7, Mo8, Mo10, Mo12, and Mo13 demonstrated potent inhibition of HBV DNA replication at submicromolar range. Copyright © 2014. Published by Elsevier Ltd.

EXAMINING THE INFLAMMATORY RESPONSES OF HAPS: THE ROLE OF OZONE AND OTHER PHTOTCHEMICAL TRANSFORMATION PRODUCTS

EPA Science Inventory

The chemistry and health effects of individual hazardous air pollutants (HAPS) have been studied for many years. Once released into the atmosphere, HAPS interact with hydroxyl radicals and ozone (created by photochemical processes), to produce many different products, whose toxic...

40 CFR 63.1569 - What are my requirements for HAP emissions from bypass lines?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true What are my requirements for HAP emissions from bypass lines? 63.1569 Section 63.1569 Protection of Environment ENVIRONMENTAL PROTECTION... HAP emissions from bypass lines? (a) What work practice standards must I meet? (1) You must meet each...

40 CFR Table 2 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2012 CFR

2012-07-01

...)(ii)(H) Application for approval Type and quantity of HAP, operating parameters No All sources emit... listed below. § 63.6(e)(3) Operation and maintenance requirements Startup, shutdown, and malfunction plan requirements No Subpart TTTT does not have any startup, shutdown, and malfunction plan requirements. § 63.6(f...

40 CFR Table 2 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2014 CFR

2014-07-01

...)(ii)(H) Application for approval Type and quantity of HAP, operating parameters No All sources emit... listed below. § 63.6(e)(3) Operation and maintenance requirements Startup, shutdown, and malfunction plan requirements No Subpart TTTT does not have any startup, shutdown, and malfunction plan requirements. § 63.6(f...

40 CFR Table 2 to Subpart Tttt of... - Leather Finishing HAP Emission Limits for Determining the Allowable HAP Loss

Code of Federal Regulations, 2013 CFR

2013-07-01

...)(ii)(H) Application for approval Type and quantity of HAP, operating parameters No All sources emit... listed below. § 63.6(e)(3) Operation and maintenance requirements Startup, shutdown, and malfunction plan requirements No Subpart TTTT does not have any startup, shutdown, and malfunction plan requirements. § 63.6(f...

The C-Terminal Fragment of the Internal 110-Kilodalton Passenger Domain of the Hap Protein of Nontypeable Haemophilus influenzae Is a Potential Vaccine Candidate

PubMed Central

Liu, Dai-Fang; Mason, Kathryn W.; Mastri, Maria; Pazirandeh, Mehran; Cutter, David; Fink, Doran L.; St. Geme, Joseph W.; Zhu, Duzhang; Green, Bruce A.

2004-01-01

Nontypeable Haemophilus influenzae is a major causative agent of bacterial otitis media in children. H. influenzae Hap autotransporter protein is an adhesin composed of an outer membrane Hapβ region and a moiety of an extracellular internal 110-kDa passenger domain called HapS. The HapS moiety promotes adherence to human epithelial cells and extracellular matrix proteins, and it also mediates bacterial aggregation and microcolony formation. A recent work (D. L. Fink, A. Z. Buscher, B. A. Green, P. Fernsten, and J. W. St. Geme, Cell. Microbiol. 5:175-186, 2003) demonstrated that HapS adhesive activity resides within the C-terminal 311 amino acids (the cell binding domain) of the protein. In this study, we immunized mice subcutaneously with recombinant proteins corresponding to the C-terminal region of HapS from H. influenzae strains N187, P860295, and TN106 and examined the resulting immune response. Antisera against the recombinant proteins from all three strains not only recognized native HapS purified from strain P860295 but also inhibited H. influenzae Hap-mediated adherence to Chang epithelial cells. Furthermore, when mice immunized intranasally with recombinant protein plus mutant cholera toxin CT-E29H were challenged with strain TN106, they were protected against nasopharyngeal colonization. These observations demonstrate that the C-terminal region of HapS is capable of eliciting cross-reacting antibodies that reduce nasopharyngeal colonization, suggesting utility as a vaccine antigen for the prevention of nontypeable H. influenzae diseases. PMID:15557618

Health Activities Project (HAP), Trial Edition III.

ERIC Educational Resources Information Center

Buller, Dave; And Others

Contained within this Health Activities Project (HAP) trial edition (set III) are a teacher information folio and numerous student activity folios which center around the idea that students in grades 5-8 can control their own health and safety. Each student folio is organized into an Overview, Health Background, Materials, Setting Up, and…

Health Activities Project (HAP): Action/Reaction Module.

ERIC Educational Resources Information Center

Buller, Dave; And Others

Contained within this Health Activities Project (HAP) learning packet are activities for children in grades 5-8. Design of the activities centers around the idea that students can control their own health and safety. Within this module are teacher and student folios describing activities in timing, improving, and practicing to improve reaction…

Thermodiffusion Coefficient Analysis of n-Dodecane /n-Hexane Mixture at Different Mass Fractions and Pressure Conditions

NASA Astrophysics Data System (ADS)

Lizarraga, Ion; Bou-Ali, M. Mounir; Santamaría, C.

2018-03-01

In this study, the thermodiffusion coefficient of n-dodecane/n-hexane binary mixture at 25 ∘C mean temperature was determined for several pressure conditions and mass fractions. The experimental technique used to determine the thermodiffusion coefficient was the thermograviational column of cylindrical configuration. In turn, thermophysical properties, such as density, thermal expansion, mass expansion and dynamic viscosity up to 10 MPa were also determined. The results obtained in this work showed a linear relation between the thermophysical properties and the pressure. Thermodiffusion coefficient values confirm a linear effect when the pressure increases. Additionally, a new correlation based on the thermodiffusion coefficient for n C12/n C6 binary mixture at 25 ∘C temperature for any mass fraction and pressures, which reproduces the data within the experimental error, was proposed.

Channel modelling for free-space optical inter-HAP links using adaptive ARQ transmission

NASA Astrophysics Data System (ADS)

Parthasarathy, S.; Giggenbach, D.; Kirstädter, A.

2014-10-01

Free-space optical (FSO) communication systems have seen significant developments in recent years due to growing need for very high data rates and tap-proof communication. The operation of an FSO link is suited to diverse variety of applications such as satellites, High Altitude Platforms (HAPs), Unmanned Aerial Vehicles (UAVs), aircrafts, ground stations and other areas involving both civil and military situations. FSO communication systems face challenges due to different effects of the atmospheric channel. FSO channel primarily suffers from scintillation effects due to Index of Refraction Turbulence (IRT). In addition, acquisition and pointing becomes more difficult because of the high directivity of the transmitted beam: Miss-pointing of the transmitted beam and tracking errors at the receiver generate additional fading of the optical signal. High Altitude Platforms (HAPs) are quasi-stationary vehicles operating in the stratosphere. The slowly varying but precisely determined time-of-flight of the Inter-HAP channel adds to its characteristics. To propose a suitable ARQ scheme, proper theoretical understanding of the optical atmospheric propagation and modeling of a specific scenario FSO channel is required. In this paper, a bi-directional symmetrical Inter-HAP link has been selected and modeled. The Inter-HAP channel model is then investigated via simulations in terms of optical scintillation induced by IRT and in presence of pointing error. The performance characteristic of the model is then quantified in terms of fading statistics from which the Packet Error Probability (PEP) is calculated. Based on the PEP characteristics, we propose suitable ARQ schemes.

CASE STUDIES: LOW-VOC/HAP WOOD FURNITURE COATINGS

EPA Science Inventory

The report gives results of a study in which wood furniture manufacturing facilities were identified that had converted at least one of their primary coating steps to low-volatile organic compound (VOC)/hazardous Air pollutant (HAP) wood furniture coatings: high-solids, water...

Determination of solid mass fraction in partially frozen hydrocarbon fuels

NASA Technical Reports Server (NTRS)

Cotterell, E. M.; Mossadegh, R.; Bruce, A. J.; Moynihan, C. T.

1986-01-01

Filtration procedures alone are insufficient to determine the amounts of crystalline solid in a partially frozen hydrocarbon distillate fraction. This is due to the nature of the solidification process by which a large amount of liquid becomes entrapped within an interconnected crystalline structure. A technique has been developed to supplement filtration methods with an independent determination of the amount of liquid in the precipitate thereby revealing the actual value of mass percent crystalline solid, %S. A non-crystallizing dye is injected into the fuel and used as a tracer during the filtration. The relative concentrations of the dye in the filtrate and precipitate fractions is subsequently detected by a spectrophotometric comparison. The filtration apparatus was assembled so that the temperature of the sample is recorded immediately above the filter. Also, a second method of calculation has been established which allows significant reduction in test time while retaining acceptable accuracy of results. Data have been obtained for eight different kerosene range hydrocarbon fuels.

40 CFR 63.1426 - Process vent requirements for determining organic HAP concentration, control efficiency, and...

Code of Federal Regulations, 2014 CFR

2014-07-01

... Production § 63.1426 Process vent requirements for determining organic HAP concentration, control efficiency..., total organic HAP, or as TOC minus methane and ethane according to the procedures specified. When... methane and ethane) concentrations in all process vent streams and primary and secondary fuels introduced...

HapMap scanning of novel human minor histocompatibility antigens.

PubMed

Kamei, Michi; Nannya, Yasuhito; Torikai, Hiroki; Kawase, Takakazu; Taura, Kenjiro; Inamoto, Yoshihiro; Takahashi, Taro; Yazaki, Makoto; Morishima, Satoko; Tsujimura, Kunio; Miyamura, Koichi; Ito, Tetsuya; Togari, Hajime; Riddell, Stanley R; Kodera, Yoshihisa; Morishima, Yasuo; Takahashi, Toshitada; Kuzushima, Kiyotaka; Ogawa, Seishi; Akatsuka, Yoshiki

2009-05-21

Minor histocompatibility antigens (mHags) are molecular targets of allo-immunity associated with hematopoietic stem cell transplantation (HSCT) and involved in graft-versus-host disease, but they also have beneficial antitumor activity. mHags are typically defined by host SNPs that are not shared by the donor and are immunologically recognized by cytotoxic T cells isolated from post-HSCT patients. However, the number of molecularly identified mHags is still too small to allow prospective studies of their clinical importance in transplantation medicine, mostly due to the lack of an efficient method for isolation. Here we show that when combined with conventional immunologic assays, the large data set from the International HapMap Project can be directly used for genetic mapping of novel mHags. Based on the immunologically determined mHag status in HapMap panels, a target mHag locus can be uniquely mapped through whole genome association scanning taking advantage of the unprecedented resolution and power obtained with more than 3 000 000 markers. The feasibility of our approach could be supported by extensive simulations and further confirmed by actually isolating 2 novel mHags as well as 1 previously identified example. The HapMap data set represents an invaluable resource for investigating human variation, with obvious applications in genetic mapping of clinically relevant human traits.

Health Activities Project (HAP): Breathing Fitness Module.

ERIC Educational Resources Information Center

Buller, Dave; And Others

Contained within this Health Activities Project (HAP) learning packet are activities for children in grades 5-8. Design of the activities centers around the idea that students can control their own health and safety. Within this module are teacher and student folios describing four activities which involve students in learning how to measure their…

Health Activities Project (HAP), Trial Edition II.

ERIC Educational Resources Information Center

Buller, Dave; And Others

Contained within this Health Activities Project (HAP) trial edition (set II) are a teacher information folio and numerous student activity folios which center around the idea that students in grades 5-8 can control their own health and safety. Each student folio is organized into a Synopsis, Health Background, Materials, Setting Up, and Activities…

Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae.

PubMed

Matsushika, Akinori; Hoshino, Tamotsu

2015-12-01

The Saccharomyces cerevisiae HAP4 gene encodes a transcription activator that plays a key role in controlling the expression of genes involved in mitochondrial respiration and reductive pathways. This work examines the effect of knockout of the HAP4 gene on aerobic ethanol production in a xylose-utilizing S. cerevisiae strain. A hap4-deleted recombinant yeast strain (B42-DHAP4) showed increased maximum concentration, production rate, and yield of ethanol compared with the reference strain MA-B42, irrespective of cultivation medium (glucose, xylose, or glucose/xylose mixtures). Notably, B42-DHAP4 was capable of producing ethanol from xylose as the sole carbon source under aerobic conditions, whereas no ethanol was produced by MA-B42. Moreover, the rate of ethanol production and ethanol yield (0.44 g/g) from the detoxified hydrolysate of wood chips was markedly improved in B42-DHAP4 compared to MA-B42. Thus, the results of this study support the view that deleting HAP4 in xylose-utilizing S. cerevisiae strains represents a useful strategy in ethanol production processes.

40 CFR 63.5390 - How do I measure the HAP content of a finish?

Code of Federal Regulations, 2010 CFR

2010-07-01

... analysis by EPA Method 311 are different from the HAP content determined by another means, the EPA Method... content analysis as determined in paragraph (a) of this section for each finish when you perform one of... 40 Protection of Environment 12 2010-07-01 2010-07-01 true How do I measure the HAP content of a...

40 CFR Table 8 to Subpart Wwww of... - Initial Compliance With Organic HAP Emissions Limits

Code of Federal Regulations, 2014 CFR

2014-07-01

.... open molding and centrifugal casting operations a. an organic HAP emissions limit shown in Tables 3 or... method meet the appropriate organic HAP contents. 2. open molding centrifugal casting, continuous... reduction is being claimed, are using direct die injection, and/or wet-area enclosures that meet the...

40 CFR Table 8 to Subpart Wwww of... - Initial Compliance With Organic HAP Emissions Limits

Code of Federal Regulations, 2013 CFR

2013-07-01

.... open molding and centrifugal casting operations a. an organic HAP emissions limit shown in Tables 3 or... method meet the appropriate organic HAP contents. 2. open molding centrifugal casting, continuous... reduction is being claimed, are using direct die injection, and/or wet-area enclosures that meet the...

40 CFR Table 8 to Subpart Wwww of... - Initial Compliance With Organic HAP Emissions Limits

Code of Federal Regulations, 2012 CFR

2012-07-01

.... open molding and centrifugal casting operations a. an organic HAP emissions limit shown in Tables 3 or... method meet the appropriate organic HAP contents. 2. open molding centrifugal casting, continuous... reduction is being claimed, are using direct die injection, and/or wet-area enclosures that meet the...

40 CFR 63.1568 - What are my requirements for HAP emissions from sulfur recovery units?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true What are my requirements for HAP emissions from sulfur recovery units? 63.1568 Section 63.1568 Protection of Environment ENVIRONMENTAL... requirements for HAP emissions from sulfur recovery units? (a) What emission limitations and work practice...

40 CFR 63.1296 - Standards for slabstock flexible polyurethane foam production-HAP ABA equipment leaks.

Code of Federal Regulations, 2013 CFR

2013-07-01

... polyurethane foam production-HAP ABA equipment leaks. 63.1296 Section 63.1296 Protection of Environment... Pollutants for Flexible Polyurethane Foam Production § 63.1296 Standards for slabstock flexible polyurethane foam production—HAP ABA equipment leaks. Each owner or operator of a new or existing slabstock affected...

40 CFR 63.1296 - Standards for slabstock flexible polyurethane foam production-HAP ABA equipment leaks.

Code of Federal Regulations, 2014 CFR

2014-07-01

... polyurethane foam production-HAP ABA equipment leaks. 63.1296 Section 63.1296 Protection of Environment... Pollutants for Flexible Polyurethane Foam Production § 63.1296 Standards for slabstock flexible polyurethane foam production—HAP ABA equipment leaks. Each owner or operator of a new or existing slabstock affected...

40 CFR 63.1295 - Standards for slabstock flexible polyurethane foam production-HAP ABA storage vessels.

Code of Federal Regulations, 2014 CFR

2014-07-01

... polyurethane foam production-HAP ABA storage vessels. 63.1295 Section 63.1295 Protection of Environment... Pollutants for Flexible Polyurethane Foam Production § 63.1295 Standards for slabstock flexible polyurethane foam production—HAP ABA storage vessels. Each owner or operator of a new or existing slabstock affected...

40 CFR 63.1295 - Standards for slabstock flexible polyurethane foam production-HAP ABA storage vessels.

Code of Federal Regulations, 2013 CFR

2013-07-01

... polyurethane foam production-HAP ABA storage vessels. 63.1295 Section 63.1295 Protection of Environment... Pollutants for Flexible Polyurethane Foam Production § 63.1295 Standards for slabstock flexible polyurethane foam production—HAP ABA storage vessels. Each owner or operator of a new or existing slabstock affected...

Crystal Structures of the Histo-Aspartic Protease (HAP) from Plasmodium falciparum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhaumik, Prasenjit; Xiao, Huogen; Parr, Charity L.

The structures of recombinant histo-aspartic protease (HAP) from malaria-causing parasite Plasmodium falciparum as apoenzyme and in complex with two inhibitors, pepstatin A and KNI-10006, were solved at 2.5-, 3.3-, and 3.05-{angstrom} resolutions, respectively. In the apoenzyme crystals, HAP forms a tight dimer not seen previously in any aspartic protease. The interactions between the monomers affect the conformation of two flexible loops, the functionally important 'flap' (residues 70-83) and its structural equivalent in the C-terminal domain (residues 238-245), as well as the orientation of helix 225-235. The flap is found in an open conformation in the apoenzyme. Unexpectedly, the active sitemore » of the apoenzyme contains a zinc ion tightly bound to His32 and Asp215 from one monomer and to Glu278A from the other monomer, with the coordination of Zn resembling that seen in metalloproteases. The flap is closed in the structure of the pepstatin A complex, whereas it is open in the complex with KNI-10006. Although the binding mode of pepstatin A is significantly different from that in other pepsin-like aspartic proteases, its location in the active site makes unlikely the previously proposed hypothesis that HAP is a serine protease. The binding mode of KNI-10006 is unusual compared with the binding of other inhibitors from the KNI series to aspartic proteases. The novel features of the HAP active site could facilitate design of specific inhibitors used in the development of antimalarial drugs.« less

40 CFR 63.4320 - By what date must I conduct the initial compliance demonstration?

Code of Federal Regulations, 2010 CFR

2010-07-01

... period, the organic HAP content of each coating and printing material you applied and the mass fraction... applicable limit in Table 1 to this subpart, and documentation that in web coating/printing operations you...

The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction

PubMed Central

Hussein, Hala E.; Bastos, Reginaldo G.; Schneider, David A.; Johnson, Wendell C.; Adham, Fatma K.; Davis, William C.; Laughery, Jacob M.; Herndon, David R.; Alzan, Heba F.

2017-01-01

Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. Babesia parasites have a complex life cycle including asexual replication in the mammalian host and sexual reproduction in the tick vector. Novel control strategies aimed at limiting transmission of the parasite are needed, but transmission blocking vaccine candidates remain undefined. Expression of HAP2 has been recognized as critical for the fertilization of parasites in the Babesia-related Plasmodium, and is a leading candidate for a transmission blocking vaccine against malaria. Hereby we identified the B. bovis hap2 gene and demonstrated that it is widely conserved and differentially transcribed during development within the tick midgut, but not by blood stage parasites. The hap2 gene was disrupted by transfecting B. bovis with a plasmid containing the flanking regions of the hap2 gene and the GPF-BSD gene under the control of the ef-1α-B promoter. Comparison of in vitro growth between a hap2-KO B. bovis clonal line and its parental wild type strain showed that HAP2 is not required for the development of B. bovis in erythrocytes. However, xanthurenic acid-in vitro induction experiments of sexual stages of parasites recovered after tick transmission resulted in surface expression of HAP2 exclusively in sexual stage induced parasites. In addition, hap2-KO parasites were not able to develop such sexual stages as defined both by morphology and by expression of the B. bovis sexual marker genes 6-Cys A and B. Together, the data strongly suggests that tick midgut stage differential expression of hap2 is associated with the development of B. bovis sexual forms. Overall these studies are consistent with a role of HAP2 in tick stages of the parasite and suggest that HAP2 is a potential candidate for a transmission blocking vaccine against bovine babesiosis. PMID:28985216

A HapMap harvest of insights into the genetics of common disease

PubMed Central

Manolio, Teri A.; Brooks, Lisa D.; Collins, Francis S.

2008-01-01

The International HapMap Project was designed to create a genome-wide database of patterns of human genetic variation, with the expectation that these patterns would be useful for genetic association studies of common diseases. This expectation has been amply fulfilled with just the initial output of genome-wide association studies, identifying nearly 100 loci for nearly 40 common diseases and traits. These associations provided new insights into pathophysiology, suggesting previously unsuspected etiologic pathways for common diseases that will be of use in identifying new therapeutic targets and developing targeted interventions based on genetically defined risk. In addition, HapMap-based discoveries have shed new light on the impact of evolutionary pressures on the human genome, suggesting multiple loci important for adapting to disease-causing pathogens and new environments. In this review we examine the origin, development, and current status of the HapMap; its prospects for continued evolution; and its current and potential future impact on biomedical science. PMID:18451988

Propellant Mass Fraction Calculation Methodology for Launch Vehicles and Application to Ares Vehicles

NASA Technical Reports Server (NTRS)

Holt, James B.; Monk, Timothy S.

2009-01-01

Propellant Mass Fraction (pmf) calculation methods vary throughout the aerospace industry. While typically used as a means of comparison between candidate launch vehicle designs, the actual pmf calculation method varies slightly from one entity to another. It is the purpose of this paper to present various methods used to calculate the pmf of launch vehicles. This includes fundamental methods of pmf calculation that consider only the total propellant mass and the dry mass of the vehicle; more involved methods that consider the residuals, reserves and any other unusable propellant remaining in the vehicle; and calculations excluding large mass quantities such as the installed engine mass. Finally, a historical comparison is made between launch vehicles on the basis of the differing calculation methodologies, while the unique mission and design requirements of the Ares V Earth Departure Stage (EDS) are examined in terms of impact to pmf.

40 CFR 63.4330 - By what date must I conduct the initial compliance demonstration?

Code of Federal Regulations, 2010 CFR

2010-07-01

... operations, the organic HAP emission rate for the initial compliance period was equal to or less than the applicable emission limit in Table 1 to this subpart and for dyeing/finishing operations, the mass fraction...

40 CFR Table 4 to Subpart Ppp of... - Known Organic HAP From Polyether Polyol Products

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 11 2010-07-01 2010-07-01 true Known Organic HAP From Polyether Polyol... CATEGORIES National Emission Standards for Hazardous Air Pollutant Emissions for Polyether Polyols Production Pt. 63, Subpt. PPP, Table 4 Table 4 to Subpart PPP of Part 63—Known Organic HAP From Polyether Polyol...

A novel inversion method to calculate the mass fraction of coated refractory black carbon using a centrifugal particle mass analyzer and single particle soot photometer

NASA Astrophysics Data System (ADS)

Irwin, M.; Broda, K.; Olfert, J. S.; Schill, G. P.; McMeeking, G. R.; Schnitzler, E.; Jäger, W.

2016-12-01

Refractory black carbon (rBC) has important atmospheric impacts due to its ability to absorb light, and its interactions with light are partly governed by the acquisition of coatings or other mixing processes. Here, a novel inversion method is presented which derives the mass fraction of coated rBC using a coupled centrifugal particle mass analyzer (CPMA) and single particle soot photometer (SP2). The CPMA selects particles of a known mass-­to-­charge ratio, and the SP2 detects the mass of rBC in each individual particle. The results of the inversion are the simultaneous number distributions of both rBC mass and total particle mass. Practically, the distribution can be integrated to find properties of the total aerosol population, for example, i) mass fraction of coating and ii) mass of coating on a particle of known total mass. This was demonstrated via smog chamber experiments. Initially, particles in the chamber were pure rBC, produced from a methane burner and passed through a diffusion dryer and thermal denuder. An organic (non-rBC) coating was then grown onto the aerosol over several hours via photooxidation with p-xylene. The CPMA-SP2 coupled system sampled the aerosol over the reaction period as the coating grew. The CPMA was sequentially stepped over a mass range from 0.3 to 28 fg and the SP2 measured the mass of rBC in each individual CPMA-classified particle. The number and mass distributions were constructed using the inversion. As expected, the mass and number distributions of rBC and total mass were equivalent for uncoated particles. As the non-rBC coating thickness increased over time, a shift in the number distribution towards higher total mass was observed. At the end of the experiment, fresh rBC (i.e. uncoated, bare particles) was injected into the chamber, creating an external mixture of coated and uncoated particles. This external mixture was clearly resolved in the number distribution of rBC and total particle mass. It is expected that the

Characterization of derived natural hydroxyapatite (HAp) obtained from different types of tilapia fish bones and scales

NASA Astrophysics Data System (ADS)

Fara, A. N. K. A.; Abdullah, H. Z.

2015-07-01

Hydroxyapatite, (HAp), Ca10(PO4)6(OH)2, is recognised as a biomaterial that is widely used for bone implant due to its chemical and structural similarity to the mineral components in human bone and enamel. The elements of HAp are primarily composed of calcium and phosphorus molar ratio of calcium to phosphorous is 1.67 capable to promote bone in-growth into prosthetic implant. Enormous amounts of by-product waste produced from fish factories generated an undesirable environmental impact. Thus, this study was conducted to obtain natural biological HAp from different types of tilapia fish bones and scales from fishery waste. Therefore, fish bones and scales can be as cheap source to produce biological HAp for medical applications. For this purpose, fish bones and scales of tilapia fish were boiled at 100°C to remove adhering meat and other impurities. Later, fish bones and scales were separated into several groups and subjected to different calcination temperatures of 800° C and 900° C for 3h respectively. Afterward, all calcined samples were crushed to form a fine powder. The XRD result revealed the presence of derived Hapfrom the samples powder and were identical with standard Hap. Thermo Gravimetric Analysis was carried out to show the thermal stability of the HAp powder from different types of fish bones and scales. SEM results show porous structure appeared in calcined samples compared to raw samples. The findings are the promising alternative to produce calcium and phosphorus from fishery wastes that beneficial to medical applications.

Mercury (Hg) in meteorites: Variations in abundance, thermal release profile, mass-dependent and mass-independent isotopic fractionation

NASA Astrophysics Data System (ADS)

Meier, Matthias M. M.; Cloquet, Christophe; Marty, Bernard

2016-06-01

We have measured the concentration, isotopic composition and thermal release profiles of Mercury (Hg) in a suite of meteorites, including both chondrites and achondrites. We find large variations in Hg concentration between different meteorites (ca. 10 ppb to 14,000 ppb), with the highest concentration orders of magnitude above the expected bulk solar system silicates value. From the presence of several different Hg carrier phases in thermal release profiles (150-650 °C), we argue that these variations are unlikely to be mainly due to terrestrial contamination. The Hg abundance of meteorites shows no correlation with petrographic type, or mass-dependent fractionation of Hg isotopes. Most carbonaceous chondrites show mass-independent enrichments in the odd-numbered isotopes 199Hg and 201Hg. We show that the enrichments are not nucleosynthetic, as we do not find corresponding nucleosynthetic deficits of 196Hg. Instead, they can partially be explained by Hg evaporation and redeposition during heating of asteroids from primordial radionuclides and late-stage impact heating. Non-carbonaceous chondrites, most achondrites and the Earth do not show these enrichments in vapor-phase Hg. All meteorites studied here have however isotopically light Hg (δ202Hg = ∼-7 to -1) relative to the Earth's average crustal values, which could suggest that the Earth has lost a significant fraction of its primordial Hg. However, the late accretion of carbonaceous chondritic material on the order of ∼2%, which has been suggested to account for the water, carbon, nitrogen and noble gas inventories of the Earth, can also contribute most or all of the Earth's current Hg budget. In this case, the isotopically heavy Hg of the Earth's crust would have to be the result of isotopic fractionation between surface and deep-Earth reservoirs.

40 CFR 63.1298 - Standards for slabstock flexible polyurethane foam production-HAP emissions from equipment cleaning.

Code of Federal Regulations, 2014 CFR

2014-07-01

... polyurethane foam production-HAP emissions from equipment cleaning. 63.1298 Section 63.1298 Protection of... Hazardous Air Pollutants for Flexible Polyurethane Foam Production § 63.1298 Standards for slabstock flexible polyurethane foam production—HAP emissions from equipment cleaning. Each owner or operator of a...

40 CFR 63.1298 - Standards for slabstock flexible polyurethane foam production-HAP emissions from equipment cleaning.

Code of Federal Regulations, 2010 CFR

2010-07-01

... polyurethane foam production-HAP emissions from equipment cleaning. 63.1298 Section 63.1298 Protection of... Pollutants for Flexible Polyurethane Foam Production § 63.1298 Standards for slabstock flexible polyurethane foam production—HAP emissions from equipment cleaning. Each owner or operator of a new or existing...

40 CFR 63.1298 - Standards for slabstock flexible polyurethane foam production-HAP emissions from equipment cleaning.

Code of Federal Regulations, 2013 CFR

2013-07-01

... polyurethane foam production-HAP emissions from equipment cleaning. 63.1298 Section 63.1298 Protection of... Hazardous Air Pollutants for Flexible Polyurethane Foam Production § 63.1298 Standards for slabstock flexible polyurethane foam production—HAP emissions from equipment cleaning. Each owner or operator of a...

40 CFR 63.1298 - Standards for slabstock flexible polyurethane foam production-HAP emissions from equipment cleaning.

Code of Federal Regulations, 2011 CFR

2011-07-01

... polyurethane foam production-HAP emissions from equipment cleaning. 63.1298 Section 63.1298 Protection of... Pollutants for Flexible Polyurethane Foam Production § 63.1298 Standards for slabstock flexible polyurethane foam production—HAP emissions from equipment cleaning. Each owner or operator of a new or existing...

40 CFR 63.1298 - Standards for slabstock flexible polyurethane foam production-HAP emissions from equipment cleaning.

Code of Federal Regulations, 2012 CFR

2012-07-01

... polyurethane foam production-HAP emissions from equipment cleaning. 63.1298 Section 63.1298 Protection of... Pollutants for Flexible Polyurethane Foam Production § 63.1298 Standards for slabstock flexible polyurethane foam production—HAP emissions from equipment cleaning. Each owner or operator of a new or existing...

Electric field-assisted formation of organically modified hydroxyapatite (ormoHAP) spheres in carboxymethylated gelatin gels.

PubMed

Heinemann, C; Heinemann, S; Kruppke, B; Worch, H; Thomas, J; Wiesmann, H P; Hanke, T

2016-10-15

A biomimetic strategy was developed in order to prepare organically modified hydroxyapatite (ormoHAP) with spherical shape. The technical approach is based on electric field-assisted migration of calcium ions and phosphate ions into a hydrogel composed of carboxymethylated gelatin. The electric field as well as the carboxymethylation using glucuronic acid (GlcA) significantly accelerates the mineralization process, which makes the process feasible for lab scale production of ormoHAP spheres and probably beyond. A further process was developed for gentle separation of the ormoHAP spheres from the gelatin gel without compromising the morphology of the mineral. The term ormoHAP was chosen since morphological analyses using electron microscopy (SEM, TEM) and element analysis (EDX, FT-IR, XRD) confirmed that carboxymethylated gelatin molecules use to act as organic templates for the formation of nanocrystalline HAP. The hydroxyapatite (HAP) crystals self-organize to form hollow spheres with diameters ranging from 100 to 500nm. The combination of the biocompatible chemical composition and the unique structure of the nanocomposites is considered to be a useful basis for future applications in functionalized degradable biomaterials. A novel bioinspired mineralization process was developed based on electric field-assisted migration of calcium and phosphate ions into biochemically carboxymethylated gelatin acting as organic template. Advantages over conventional hydroxyapatite include particle size distribution and homogeneity as well as achievable mechanical properties of relevant composites. Moreover, specifically developed calcium ion or phosphate ion release during degradation can be useful to adjust the fate of bone cells in order to manipulate remodeling processes. The hollow structure of the spheres can be useful for embedding drugs in the core, encapsulated by the highly mineralized outer shell. In this way, controlled drug release could be achieved, which enables

Galaxy Cluster Gas Mass Fractions From Sunyaev-Zel'dovich Effect Measurement: Constraints on Omega_M

NASA Technical Reports Server (NTRS)

Grego, Laura; Carlstrom, John E.; Reese, Erik D.; Holder, Gilbert P.; Holzapfel, William L.; Joy, Marshall K.; Mohr, Joseph J.; Patel, Sandeep; Rose, M. Franklin (Technical Monitor)

2001-01-01

Using sensitive centimeter-wave receivers mounted on the Owens Valley Radio Observatory and Berkeley-Illinois-Maryland-Association millimeter arrays, we have obtained interferometric measurements of the Sunyaev-Zei'dovich (SZ) effect toward massive galaxy clusters. We use the SZ data to determine the pressure distribution of the cluster gas and, in combination with published X-ray temperatures, to infer the gas mass and total gravitational mass of 18 clusters. The gas mass fraction, fg, is calculated for each cluster, and is extrapolated to the fiducial radius r_{500} using the results of numerical simulations. The mean f_g within r_{500} is 0.081 + 0.009 - 0.011/(h_{100} (statistical uncertainty at 68% confidence level, assuming OmegaM=0.3, OmegaL=0.7). We discuss possible sources of systematic errors in the mean f 9 measurement. We derive an upper limit for OmegaM from this sample under the assumption that the mass composition of clusters within r_{500} reflects the universal mass composition: Omega_M h mass f on cosmology through the angular diameter distance and the r_{500} correction factors. For a flat universe (Omegal, = 1 - OmegaM) and h=0.7, we find the measured gas mass fractions are consistent with OmegaM less than 0.40, at 68% confidence. Including estimates of the baryons contained in galaxies and the baryons which failed to become bound during the cluster formation process, we find OmegaM\\approximately 0.25.

40 CFR Table 4 to Subpart Ppp of... - Known Organic HAP From Polyether Polyol Products

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 11 2011-07-01 2011-07-01 false Known Organic HAP From Polyether Polyol Products 4 Table 4 to Subpart PPP of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION... Production Pt. 63, Subpt. PPP, Table 4 Table 4 to Subpart PPP of Part 63—Known Organic HAP From Polyether...

40 CFR Table 4 to Subpart Ppp of... - Known Organic HAP From Polyether Polyol Products

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 12 2014-07-01 2014-07-01 false Known Organic HAP From Polyether Polyol Products 4 Table 4 to Subpart PPP of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION... Polyether Polyols Production Pt. 63, Subpt. PPP, Table 4 Table 4 to Subpart PPP of Part 63—Known Organic HAP...

40 CFR Table 4 to Subpart Ppp of... - Known Organic HAP From Polyether Polyol Products

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 12 2013-07-01 2013-07-01 false Known Organic HAP From Polyether Polyol Products 4 Table 4 to Subpart PPP of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION... Polyether Polyols Production Pt. 63, Subpt. PPP, Table 4 Table 4 to Subpart PPP of Part 63—Known Organic HAP...

40 CFR Table 4 to Subpart Ppp of... - Known Organic HAP From Polyether Polyol Products

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 12 2012-07-01 2011-07-01 true Known Organic HAP From Polyether Polyol Products 4 Table 4 to Subpart PPP of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Pt. 63, Subpt. PPP, Table 4 Table 4 to Subpart PPP of Part 63—Known Organic HAP From Polyether Polyol...

40 CFR Appendix: Table 1 to... - List of Hazardous Air Pollutants (HAP) for Subpart HHH

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 11 2010-07-01 2010-07-01 true List of Hazardous Air Pollutants (HAP) for Subpart HHH Table Appendix: Table 1 to Subpart HHH of Part 63 Protection of Environment... HHH of Part 63—List of Hazardous Air Pollutants (HAP) for Subpart HHH CAS Number a Chemical name 75070...

EEG and ERP profiles in the high alcohol preferring (HAP) and low alcohol preferring (LAP) mice: relationship to ethanol preference.

PubMed

Slawecki, Craig J; Grahame, Nicholas J; Roth, Jennifer; Katner, Simon N; Ehlers, C L

2003-01-31

Neurophysiological measures, such as decreased P300 amplitude and altered EEG alpha activity, have been associated with increased alcoholism risk. The purpose of the present study was to extend the assessment of the neurophysiological indices associated with alcohol consumption to a recently developed mouse model of high ethanol consumption, the first replicate line of high alcohol preferring (HAP-1) and low alcohol preferring (LAP-1) mice. Male HAP-1, LAP-1, and HS mice from the Institute for Behavioral Genetics at the University of Colorado Health Science Center (i.e., HS/Ibg mice) were implanted with cortical electrodes. EEG activity, and event related potentials (ERPs) were then examined. Following electrophysiological assessment, ethanol preference was assessed to examine the relationship between neurophysiological indices and ethanol consumption. EEG analyses revealed that HAPs and HS/Ibgs had greater peak frequency in the 2-4-Hz band and lower peak frequency in the 6-8- and 1-50-Hz bands of the cortical EEG compared to LAPs. Compared to HAPs, LAPs and HS/Ibgs had decreased peak EEG frequency in the 8-16-Hz band. Decreased parietal cortical power from 8 to 50 Hz was associated with high initial ethanol preference in HAP mice. In regards to ERPs, P1 amplitude was greater in HAPs compared to both LAPs and HS/Ibgs and the P3 latency in LAPs was decreased compared to both HAPs and HS/Ibgs. As expected, HAPs consumed more ethanol and had higher ethanol preference than LAPs and HS/Ibgs. There were no significant differences in ethanol intake or preference between HS/Ibgs and LAPs. These data indicate that selective breeding of the HAP and LAP lines has resulted in the divergence of EEG and ERP phenotypes. The differences observed suggest that increased cortical P1 amplitude and altered cortical EEG activity in the 8-50-Hz frequency range may be neurophysiological 'risk factors' associated with high ethanol consumption in mice. Decreased P3 latency in LAPs compared

40 CFR 63.1297 - Standards for slabstock flexible polyurethane foam production-HAP ABA emissions from the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... polyurethane foam production-HAP ABA emissions from the production line. 63.1297 Section 63.1297 Protection of... Hazardous Air Pollutants for Flexible Polyurethane Foam Production § 63.1297 Standards for slabstock flexible polyurethane foam production—HAP ABA emissions from the production line. (a) Each owner or...

40 CFR 63.1297 - Standards for slabstock flexible polyurethane foam production-HAP ABA emissions from the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... polyurethane foam production-HAP ABA emissions from the production line. 63.1297 Section 63.1297 Protection of... Hazardous Air Pollutants for Flexible Polyurethane Foam Production § 63.1297 Standards for slabstock flexible polyurethane foam production—HAP ABA emissions from the production line. (a) Each owner or...

Experimental Study on 3D Chi - Hap Scaffolds for Thyroid Cartilage Repairing

NASA Astrophysics Data System (ADS)

Sun, Nannan; Shi, Tingchun; Fan, Yuan; Hu, Binbin

2018-01-01

Due to the limitation of self-repairing capability for cartilage injury, the construction of tissue engineering in vitro has been an ideal treatment to repair tissue injury. In this paper, hydroxyapatite (Hap) and chitosan (Chi) were selected to fabricate the scaffold through low temperature deposition manufacturing (LDM) technique. The scaffold was characterized with interconnected structure and high porosity, as well as lower toxicity to cells (TDC-5-EGPE). Animal experiment was performed, Twelve white New Zealand rabbits were randomly divided into two groups, the side of the thyroid cartilage was removed, Chi-HAP composite scaffold was implanted into the cartilage defect as the experimental group A. Group B was treated for thyroid cartilage defects without any treatment. After 10 weeks, hematoxylin-eosin (HE) staining and S-O staining were carried out on the injured tissues. The result showed that newborn chondrocytes were found in repaired areas for group A, and there are no new cells found for group B. Therefore, Chi-HAP composite scaffolds formed by LDM possess biological activity for repairing injury cartilage.

All-optical two-way relaying free-space optical communications for HAP-based broadband backhaul networks

NASA Astrophysics Data System (ADS)

Vu, Minh Q.; Nguyen, Nga T. T.; Pham, Hien T. T.; Dang, Ngoc T.

2018-03-01

High-altitude platforms (HAPs) are flexible, non-pollutant and cost-effective infrastructures compared to satellite or old terrestrial systems. They are being researched and developed widely in Europe, USA, Japan, Korea, and so on. However, the current limited data rates and the overload of radio frequency (RF) spectrum are problems which the developers for HAPs are confronting because most of them use RF links to communicate with the ground stations (GSs) or each other. In this paper, we propose an all-optical two-way half-duplex relaying free-space optical (FSO) communication for HAP-based backhaul networks, which connect the base transceiver station (BTS) to the core network (CN) via a single HAP. Our proposed backhaul solution can be deployed quickly and flexibly for disaster relief and for serving users in both urban environments and remote areas. The key subsystem of HAP is an optical regenerate-and-forward (ORF) equipped with an optical hard-limiter (OHL) and an optical XOR gate to perform all-optical processing and help mitigate the background noise. In addition, two-way half-duplex relaying can be provided thanks to the use of network coding scheme. The closed-form expression for the bit error rate (BER) of our proposed system under the effect of path loss, atmospheric turbulence, and noise induced by the background light is formulated. The numerical results are demonstrated to prove the feasibility of our proposed system with the verification by using Monte-Carlo (M-C) simulations.

Cryopreservation of Hair-Follicle Associated Pluripotent (HAP) Stem Cells Maintains Differentiation and Hair-Growth Potential.

PubMed

Hoffman, Robert M; Kajiura, Satoshi; Cao, Wenluo; Liu, Fang; Amoh, Yasuyuki

2016-01-01

Hair follicles contain nestin-expressing pluripotent stem cells which originate above the bulge area of the follicle, below the sebaceous gland. We have termed these cells hair follicle-associated pluripotent (HAP) stem cells. We have established efficient cryopreservation methods of the hair follicle that maintain the pluripotency of HAP stem cells as well as hair growth. We cryopreserved the whole hair follicle by slow-rate cooling in TC-Protector medium or in DMSO-containing medium and storage in liquid nitrogen or at -80 °C. After thawing and culture of the cryopreserved whisker follicles, growing HAP stem cells formed hair spheres. The hair spheres contained cells that differentiated to neurons, glial cells, and other cell types. The hair spheres derived from slow-cooling cryopreserved hair follicles were as pluripotent as hair spheres from fresh hair follicles. We have also previously demonstrated that cryopreserved mouse whisker hair follicles maintain their hair-growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. DMSO-cryopreserved hair follicles also maintained the HAP stem cells, evidenced by P75 ntr expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair-shaft growth of cryopreserved hair follicles. HAP stem cells can be used for nerve and spinal-cord repair. This biobanking of hair follicles can allow each patient the potential for their own stem cell use for regenerative medicine or hair transplantation.

Health Activities Project (HAP): Sight and Sound Module.

ERIC Educational Resources Information Center

Buller, Dave; And Others

Contained within this Health Activities Project (HAP) learning packet are activities for children in grades 5-8. Design of the activities centers around the idea that students can control their own health and safety. Within this module are teacher and student folios describing six activities which involve students in restricting their vision by…

40 CFR 63.1297 - Standards for slabstock flexible polyurethane foam production-HAP ABA emissions from the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... polyurethane foam production-HAP ABA emissions from the production line. 63.1297 Section 63.1297 Protection of... Pollutants for Flexible Polyurethane Foam Production § 63.1297 Standards for slabstock flexible polyurethane... § 63.1293(a)(1) shall control HAP ABA emissions from the slabstock polyurethane foam production line in...

40 CFR 63.1297 - Standards for slabstock flexible polyurethane foam production-HAP ABA emissions from the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... polyurethane foam production-HAP ABA emissions from the production line. 63.1297 Section 63.1297 Protection of... Pollutants for Flexible Polyurethane Foam Production § 63.1297 Standards for slabstock flexible polyurethane... § 63.1293(a)(1) shall control HAP ABA emissions from the slabstock polyurethane foam production line in...

40 CFR 63.1297 - Standards for slabstock flexible polyurethane foam production-HAP ABA emissions from the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... polyurethane foam production-HAP ABA emissions from the production line. 63.1297 Section 63.1297 Protection of... Pollutants for Flexible Polyurethane Foam Production § 63.1297 Standards for slabstock flexible polyurethane... § 63.1293(a)(1) shall control HAP ABA emissions from the slabstock polyurethane foam production line in...

Molar mass fractionation in aqueous two-phase polymer solutions of dextran and poly(ethylene glycol).

PubMed

Zhao, Ziliang; Li, Qi; Ji, Xiangling; Dimova, Rumiana; Lipowsky, Reinhard; Liu, Yonggang

2016-06-24

Dextran and poly(ethylene glycol) (PEG) in phase separated aqueous two-phase systems (ATPSs) of these two polymers, with a broad molar mass distribution for dextran and a narrow molar mass distribution for PEG, were separated and quantified by gel permeation chromatography (GPC). Tie lines constructed by GPC method are in excellent agreement with those established by the previously reported approach based on density measurements of the phases. The fractionation of dextran during phase separation of ATPS leads to the redistribution of dextran of different chain lengths between the two phases. The degree of fractionation for dextran decays exponentially as a function of chain length. The average separation parameters, for both dextran and PEG, show a crossover from mean field behavior to Ising model behavior, as the critical point is approached. Copyright © 2016 Elsevier B.V. All rights reserved.

The Healthy Activity Program (HAP), a lay counsellor-delivered brief psychological treatment for severe depression, in primary care in India: a randomised controlled trial.

PubMed

Patel, Vikram; Weobong, Benedict; Weiss, Helen A; Anand, Arpita; Bhat, Bhargav; Katti, Basavraj; Dimidjian, Sona; Araya, Ricardo; Hollon, Steve D; King, Michael; Vijayakumar, Lakshmi; Park, A-La; McDaid, David; Wilson, Terry; Velleman, Richard; Kirkwood, Betty R; Fairburn, Christopher G

2017-01-14

Although structured psychological treatments are recommended as first-line interventions for depression, only a small fraction of people globally receive these treatments because of poor access in routine primary care. We assessed the effectiveness and cost-effectiveness of a brief psychological treatment (Healthy Activity Program [HAP]) for delivery by lay counsellors to patients with moderately severe to severe depression in primary health-care settings. In this randomised controlled trial, we recruited participants aged 18-65 years scoring more than 14 on the Patient Health Questionnaire 9 (PHQ-9) indicating moderately severe to severe depression from ten primary health centres in Goa, India. Pregnant women or patients who needed urgent medical attention or were unable to communicate clearly were not eligible. Participants were randomly allocated (1:1) to enhanced usual care (EUC) alone or EUC combined with HAP in randomly sized blocks (block size four to six [two to four for men]), stratified by primary health centre and sex, and allocation was concealed with use of sequential numbered opaque envelopes. Physicians providing EUC were masked. Primary outcomes were depression symptom severity on the Beck Depression Inventory version II and remission from depression (PHQ-9 score of <10) at 3 months in the intention-to-treat population, assessed by masked field researchers. Secondary outcomes were disability, days unable to work, behavioural activation, suicidal thoughts or attempts, intimate partner violence, and resource use and costs of illness. We assessed serious adverse events in the per-protocol population. This trial is registered with the ISRCTN registry, number ISRCTN95149997. Between Oct 28, 2013, and July 29, 2015, we enrolled and randomly allocated 495 participants (247 [50%] to the EUC plus HAP group [two of whom were subsequently excluded because of protocol violations] and 248 [50%] to the EUC alone group), of whom 466 (95%) completed the 3 month

Case study project: The use of low-VOC/HAP coatings at wood furniture manufacturing facilities. Report for March 1995--March 1999

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marshall, A.M.; Jones, J.W.; Fields, J.L.

1999-07-01

The paper discusses a study of pollution prevention and the use of low-VOC/HAP (volatile organic compound/hazardous air pollutant) coatings at wood furniture manufacturing facilities. The study is to identify wood furniture and cabinet manufacturing facilities that have converted to low-VOC/HAP coatings and to develop case studies for those facilities. The case studies include a discussion of the types of products each facility manufactures; the types of low-VOC/HAP coatings each facility is using; problems encountered in converting to low-VOC/HAP coatings; equipment changes that were required; costs associated with the conversion process, including capital costs associated with equipment purchases, research and developmentmore » costs, and operating costs such as operator training in new application techniques;advantages/ disadvantages of the low-VOC/HAP coatings; and customer feedback on products finished with the low-VOC/HAP coatings. The paper discusses the progress of the study and pollution prevention options at wood furniture manufacturing facilities.« less

40 CFR 63.3941 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2011 CFR

2011-07-01

... the atmosphere, you may use the alternative method contained in appendix A to subpart PPPP of this... alternative method in appendix A to subpart PPPP of this part, as a substitute for the mass fraction of organic HAP. (3) Alternative method. You may use an alternative test method for determining the mass...

Discovery and Pre-Clinical Characterization of Third-Generation 4-H Heteroaryldihydropyrimidine (HAP) Analogues as Hepatitis B Virus (HBV) Capsid Inhibitors.

PubMed

Qiu, Zongxing; Lin, Xianfeng; Zhang, Weixing; Zhou, Mingwei; Guo, Lei; Kocer, Buelent; Wu, Guolong; Zhang, Zhisen; Liu, Haixia; Shi, Houguang; Kou, Buyu; Hu, Taishan; Hu, Yimin; Huang, Mengwei; Yan, S Frank; Xu, Zhiheng; Zhou, Zheng; Qin, Ning; Wang, Yue Fen; Ren, Shuang; Qiu, Hongxia; Zhang, Yuxia; Zhang, Yi; Wu, Xiaoyue; Sun, Kai; Zhong, Sheng; Xie, Jianxun; Ottaviani, Giorgio; Zhou, Yuan; Zhu, Lina; Tian, Xiaojun; Shi, Liping; Shen, Fang; Mao, Yi; Zhou, Xue; Gao, Lu; Young, John A T; Wu, Jim Zhen; Yang, Guang; Mayweg, Alexander V; Shen, Hong C; Tang, Guozhi; Zhu, Wei

2017-04-27

Described herein are the discovery and structure-activity relationship (SAR) studies of the third-generation 4-H heteroaryldihydropyrimidines (4-H HAPs) featuring the introduction of a C6 carboxyl group as novel HBV capsid inhibitors. This new series of 4-H HAPs showed improved anti-HBV activity and better drug-like properties compared to the first- and second-generation 4-H HAPs. X-ray crystallographic study of analogue 12 (HAP_R01) with Cp149 Y132A mutant hexamer clearly elucidated the role of C6 carboxyl group played for the increased binding affinity, which formed strong hydrogen bonding interactions with capsid protein and coordinated waters. The representative analogue 10 (HAP_R10) was extensively characterized in vitro (ADMET) and in vivo (mouse PK and PD) and subsequently selected for further development as oral anti-HBV infection agent.

Mining the human plasma proteome with three-dimensional strategies by high-resolution Quadrupole Orbitrap Mass Spectrometry.

PubMed

Zhao, Yan; Chang, Cheng; Qin, Peibin; Cao, Qichen; Tian, Fang; Jiang, Jing; Li, Xianyu; Yu, Wenfeng; Zhu, Yunping; He, Fuchu; Ying, Wantao; Qian, Xiaohong

2016-01-21

Human plasma is a readily available clinical sample that reflects the status of the body in normal physiological and disease states. Although the wide dynamic range and immense complexity of plasma proteins are obstacles, comprehensive proteomic analysis of human plasma is necessary for biomarker discovery and further verification. Various methods such as immunodepletion, protein equalization and hyper fractionation have been applied to reduce the influence of high-abundance proteins (HAPs) and to reduce the high level of complexity. However, the depth at which the human plasma proteome has been explored in a relatively short time frame has been limited, which impedes the transfer of proteomic techniques to clinical research. Development of an optimal strategy is expected to improve the efficiency of human plasma proteome profiling. Here, five three-dimensional strategies combining HAP depletion (the 1st dimension) and protein fractionation (the 2nd dimension), followed by LC-MS/MS analysis (the 3rd dimension) were developed and compared for human plasma proteome profiling. Pros and cons of the five strategies are discussed for two issues: HAP depletion and complexity reduction. Strategies A and B used proteome equalization and tandem Seppro IgY14 immunodepletion, respectively, as the first dimension. Proteome equalization (strategy A) was biased toward the enrichment of basic and low-molecular weight proteins and had limited ability to enrich low-abundance proteins. By tandem removal of HAPs (strategy B), the efficiency of HAP depletion was significantly increased, whereas more off-target proteins were subtracted simultaneously. In the comparison of complexity reduction, strategy D involved a deglycosylation step before high-pH RPLC separation. However, the increase in sequence coverage did not increase the protein number as expected. Strategy E introduced SDS-PAGE separation of proteins, and the results showed oversampling of HAPs and identification of fewer

40 CFR Table 8 to Subpart Uuu of... - Organic HAP Emission Limits for Catalytic Cracking Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Organic HAP Emission Limits for Catalytic Cracking Units 8 Table 8 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 8 Table 8 to Subpart UUU of Part 63—Organic HAP Emission Limits for Catalytic Cracking...

40 CFR Table 8 to Subpart Uuu of... - Organic HAP Emission Limits for Catalytic Cracking Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Organic HAP Emission Limits for Catalytic Cracking Units 8 Table 8 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 8 Table 8 to Subpart UUU of Part 63—Organic HAP Emission Limits for Catalytic Cracking...

40 CFR Table 15 to Subpart Uuu of... - Organic HAP Emission Limits for Catalytic Reforming Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Organic HAP Emission Limits for Catalytic Reforming Units 15 Table 15 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 15 Table 15 to Subpart UUU of Part 63—Organic HAP Emission Limits for Catalytic...

40 CFR Table 22 to Subpart Uuu of... - Inorganic HAP Emission Limits for Catalytic Reforming Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Inorganic HAP Emission Limits for Catalytic Reforming Units 22 Table 22 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 22 Table 22 to Subpart UUU of Part 63—Inorganic HAP Emission Limits for Catalytic...

40 CFR Table 22 to Subpart Uuu of... - Inorganic HAP Emission Limits for Catalytic Reforming Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Inorganic HAP Emission Limits for Catalytic Reforming Units 22 Table 22 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 22 Table 22 to Subpart UUU of Part 63—Inorganic HAP Emission Limits for Catalytic...

40 CFR Table 15 to Subpart Uuu of... - Organic HAP Emission Limits for Catalytic Reforming Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Organic HAP Emission Limits for Catalytic Reforming Units 15 Table 15 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 15 Table 15 to Subpart UUU of Part 63—Organic HAP Emission Limits for Catalytic...

40 CFR Table 8 to Subpart Uuu of... - Organic HAP Emission Limits for Catalytic Cracking Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Organic HAP Emission Limits for Catalytic Cracking Units 8 Table 8 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 8 Table 8 to Subpart UUU of Part 63—Organic HAP Emission Limits for Catalytic Cracking...

40 CFR Table 22 to Subpart Uuu of... - Inorganic HAP Emission Limits for Catalytic Reforming Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Inorganic HAP Emission Limits for Catalytic Reforming Units 22 Table 22 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 22 Table 22 to Subpart UUU of Part 63—Inorganic HAP Emission Limits for Catalytic...

40 CFR Table 15 to Subpart Uuu of... - Organic HAP Emission Limits for Catalytic Reforming Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Organic HAP Emission Limits for Catalytic Reforming Units 15 Table 15 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 15 Table 15 to Subpart UUU of Part 63—Organic HAP Emission Limits for Catalytic...

Failure behavior of plasma-sprayed HAp coating on commercially pure titanium substrate in simulated body fluid (SBF) under bending load.

PubMed

Laonapakul, Teerawat; Rakngarm Nimkerdphol, Achariya; Otsuka, Yuichi; Mutoh, Yoshiharu

2012-11-01

Four point bending tests with acoustic emission (AE) monitoring were conducted for evaluating failure behavior of the plasma-sprayed hydroxyapatite (HAp) top coat on commercially pure titanium (cp-Ti) plate with and without mixed HAp/Ti bond coat. Effect of immersion in simulated body fluid (SBF) on failure behavior of the coated specimen was also investigated by immersing the specimen in SBF. The AE patterns obtained from the bending test of the HAp coating specimens after a week immersion in SBF clearly showed the earlier stage of delamination and spallation of the coating layer compared to those without immersion in SBF. It was also found that the bond coating improved failure resistance of the HAp coating specimen compared to that without the bond coat. Four point bend fatigue tests under ambient and SBF environments were also conducted with AE monitoring during the entire fatigue test for investigating the influence of SBF environment on fatigue failure behavior of the HAp coating specimen with the mixed HAp/Ti bond coat. The specimens tested at a stress amplitude of 120 MPa under both ambient and SBF environments could survive up to 10⁷ cycles without spallation of HAp coating layer. The specimens tested under SBF environment and those tested under ambient environment after immersion in SBF showed shorter fatigue life compared to those tested under ambient environment without SBF immersion. Micro-cracks nucleated in the coating layer in the early stage of fatigue life and then propagated into the cp-Ti substrate in the intermediate stage, which unstably propagated to failure in the final stage. It was found from the XRD analysis that the dissolution of the co-existing phases and the precipitation of the HAp phase were taken place during immersion in SBF. During this process, the co-existing phases disappeared from the coating layer and the HAp phase fully occupied the coating layer. The degradation of bending strength and fatigue life of the HAp coating

Use of a crossed high alcohol preferring (cHAP) mouse model with the NIAAA-model of chronic-binge ethanol intake to study liver injury.

PubMed

Thompson, Kyle J; Nazari, Shayan S; Jacobs, W Carl; Grahame, Nicholas J; McKillop, Iain H

2017-11-01

This study sought to compare mice bred to preferentially consume high amounts of alcohol (crossed-high alcohol preferring, cHAP) to c57BL/6 (C57) mice using a chronic-binge ethanol ingestion model to induce alcoholic liver disease (ALD). Male C57 and cHAP mice were randomized to a Lieber-DeCarli control (LDC) diet, Lieber-DeCarli 5% (v/v) ethanol (LDE) diet or free-choice between 10% (v/v) ethanol in drinking water (EtOH-DW) and DW. After 4 weeks mice were gavaged with either 9 g/kg maltose-dextrin (LDC+MD) or 5 g/kg EtOH (LDE+Binge, EtOH-DW+Binge). Nine hours later tissue and serum were collected and analyzed. cHAP mice on EtOH-DW consumed significantly more ethanol than cHAP or C57 mice maintained on LDE. However, cHAP and C57 mice on the LDE+Binge regiment had greater hepatosteatosis and overall degree of liver injury compared to EtOH-DW+Binge. Changes in pro-inflammatory gene expression was more pronounced in cHAP mice than C57 mice. Analysis of liver enzymes revealed a robust induction of CYP2E1 in C57 and cHAP mice maintained on EtOH-DW+Binge or LDE+Binge. However, while C57 mice exhibited higher basal hepatic glutathione than cHAP mice, these mice appeared more susceptible to oxidative stress following LDE+Binge than cHAP counterparts. Despite cHAP mice consuming more total ethanol prior to gavage when maintained on EtOH-DW, LDE followed by gavage created a more severe model of ALD in both C57 and cHAP mice. These data suggest factors other than total amount of alcohol consumed affect degree of ALD development in the chronic-binge model in cHAP mice. cHAP mice voluntarily consume high amounts of ethanol and exhibited hepatic injury when subject to chronic-binge ethanol feeding with the Lieber-DeCarli diet. However, hepatic injury was reduced in cHAP mice in a chronic-binge model following voluntary high ethanol consumption in drinking water. © The Author 2017. Medical Council on Alcohol and Oxford University Press. All rights reserved.

HapX-Mediated Iron Homeostasis Is Essential for Rhizosphere Competence and Virulence of the Soilborne Pathogen Fusarium oxysporum[C][W][OA

PubMed Central

López-Berges, Manuel S.; Capilla, Javier; Turrà, David; Schafferer, Lukas; Matthijs, Sandra; Jöchl, Christoph; Cornelis, Pierre; Guarro, Josep; Haas, Hubertus; Di Pietro, Antonio

2012-01-01

Soilborne fungal pathogens cause devastating yield losses and are highly persistent and difficult to control. During the infection process, these organisms must cope with limited availability of iron. Here we show that the bZIP protein HapX functions as a key regulator of iron homeostasis and virulence in the vascular wilt fungus Fusarium oxysporum. Deletion of hapX does not affect iron uptake but causes derepression of genes involved in iron-consuming pathways, leading to impaired growth under iron-depleted conditions. F. oxysporum strains lacking HapX are reduced in their capacity to invade and kill tomato (Solanum lycopersicum) plants and immunodepressed mice. The virulence defect of ΔhapX on tomato plants is exacerbated by coinoculation of roots with a biocontrol strain of Pseudomonas putida, but not with a siderophore-deficient mutant, indicating that HapX contributes to iron competition of F. oxysporum in the tomato rhizosphere. These results establish a conserved role for HapX-mediated iron homeostasis in fungal infection of plants and mammals. PMID:22968717

40 CFR 63.7944 - How do I determine the maximum HAP vapor pressure of my remediation material?

Code of Federal Regulations, 2010 CFR

2010-07-01

... vapor pressure of my remediation material? 63.7944 Section 63.7944 Protection of Environment... Pollutants: Site Remediation Performance Tests § 63.7944 How do I determine the maximum HAP vapor pressure of my remediation material? (a) You must determine the maximum HAP vapor pressure of your remediation...

40 CFR 63.4321 - How do I demonstrate initial compliance with the emission limitations?

Code of Federal Regulations, 2013 CFR

2013-07-01

... PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES (CONTINUED) National Emission Standards for Hazardous Air Pollutants... dyeing or finishing material with a mass fraction of organic HAP that exceeds the applicable emission...

Augmentation of Quasi-Zenith Satellite Positioning System Using High Altitude Platforms Systems (HAPS)

NASA Astrophysics Data System (ADS)

Tsujii, Toshiaki; Harigae, Masatoshi

Recently, some feasibility studies on a regional positioning system using the quasi-zenith satellites and the geostationary satellites have been conducted in Japan. However, the geometry of this system seems to be unsatisfactory in terms of the positioning accuracy in north-south direction. In this paper, an augmented satellite positioning system by the High Altitude Platform Systems (HAPS) is proposed since the flexibility of the HAPS location is effective to improve the geometry of satellite positioning system. The improved positioning performance of the augmented system is also demonstrated.

Age at onset in Huntington's disease: replication study on the association of HAP1.

PubMed

Karadima, Georgia; Dimovasili, Christina; Koutsis, Georgios; Vassilopoulos, Demetris; Panas, Marios

2012-11-01

In recent years two association studies investigating the HAP1 T441M (rs4523977) polymorphism as a potential modifying factor of the age at onset (AAO) of Huntington's disease (HD), have been reported. Initially evidence for association was found between the M441 risk allele and the AAO. Subsequently, a second study, although failing to replicate these findings, found evidence for association between the same risk allele and AAO of motor symptoms (mAAO). In the present study, the role of the HAP1 T441M polymorphism as a modifier of the AAO in HD was investigated in a cohort of 298 Greek HD patients. In this cohort the CAG repeat number accounted for 55% of the variance in AAO. No association was found between the HAP1 T441M polymorphism and the AAO of HD. © 2012 Elsevier Ltd. All rights reserved.

Study on the removal of organic micropollutants from aqueous and ethanol solutions by HAP membranes with tunable hydrophilicity and hydrophobicity.

PubMed

He, Junyong; Li, Yulian; Cai, Xingguo; Chen, Kai; Zheng, Hejing; Wang, Chengming; Zhang, Kaisheng; Lin, Dongyue; Kong, Lingtao; Liu, Jinhuai

2017-05-01

A biocompatible and uniquely defined hydroxyapatite (HAP) adsorption membrane with a sandwich structure was developed for the removal of organic micropollutants for the first time. Both the adsorption and membrane technique were used for the removal of organic micropollutants. The hydrophilicity and hydrophobicity of the HAP adsorbent and membrane were tunable by controlling the surface structure of HAP. The adsorption of organic micropollutants on the HAP adsorbent was studied in batch experiments. The adsorption process was fit with the Freundlich model, while the adsorption kinetics followed the pseudo-second-order model. The HAP membrane could remove organic micropollutants effectively by dynamic adsorption in both aqueous and ethanol solutions. The removal efficiencies of organic micropollutants depended on the solution composition, membrane thickness and hydrophilicity, flow rate, and the initial concentration of organic micropollutants. The adsorption capacities of the HAP membrane with a sandwich structure (membrane thickness was 0.3 mm) were 6700, 6510, 6310, 5960, 5490, 5230, 4980 and 4360 L m -2 for 1-naphthyl amine, 2-naphthol, bisphenol S, propranolol hydrochloride, metolachlor, ethinyl oestradiol, 2,4-dichlorophenol and bisphenol A, respectively, when the initial concentration was 3.0 mg L -1 . The biocompatible HAP adsorption membrane can be easily regenerated by methanol and was thus demonstrated to be a novel concept for the removal of organic micropollutants from both aqueous and organic solutions. Copyright © 2017 Elsevier Ltd. All rights reserved.

40 CFR 63.5796 - What are the organic HAP emissions factor equations in Table 1 to this subpart, and how are they...

Code of Federal Regulations, 2014 CFR

2014-07-01

... Organic Hap Emissions Factors for Open Molding and Centrifugal Casting § 63.5796 What are the organic HAP... emissions factors. Equations are available for each open molding operation and centrifugal casting operation... incorporated in the facility's air emissions permit and are based on actual facility HAP emissions test data...

40 CFR 63.5796 - What are the organic HAP emissions factor equations in Table 1 to this subpart, and how are they...

Code of Federal Regulations, 2013 CFR

2013-07-01

... Organic Hap Emissions Factors for Open Molding and Centrifugal Casting § 63.5796 What are the organic HAP... emissions factors. Equations are available for each open molding operation and centrifugal casting operation... incorporated in the facility's air emissions permit and are based on actual facility HAP emissions test data...

40 CFR 63.5796 - What are the organic HAP emissions factor equations in Table 1 to this subpart, and how are they...

Code of Federal Regulations, 2012 CFR

2012-07-01

... Organic Hap Emissions Factors for Open Molding and Centrifugal Casting § 63.5796 What are the organic HAP... emissions factors. Equations are available for each open molding operation and centrifugal casting operation... incorporated in the facility's air emissions permit and are based on actual facility HAP emissions test data...

Hair Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair.

PubMed

Liu, Fang; Hoffman, Robert M

2018-01-01

The stem cell marker, nestin, is expressed in the hair follicle, both in cells in the bulge area (BA) and the dermal papilla (DP). Nestin-expressing hair follicle-associated-pluripotent (HAP) stem cells of both the BA and DP have been previously shown to be able to form neurons, heart muscle cells, and other non-follicle cell types. The ability of the nestin-expressing HAP stem cells from the BA and DP to repair spinal cord injury was compared. Nestin-expressing HAP stem cells from both the BA and DP grew very well on Gelfoam ® . The HAP stem cells attached to the Gelfoam ® within 1 h. They grew along the grids of the Gelfoam ® during the first 2 or 3 days. Later they spread into the Gelfoam ® . After transplantation of Gelfoam ® cultures of nestin-expressing BA or DP HAP stem cells into the injured spinal cord (including the Gelfoam ® ) nestin-expressing BA and DP cells were observed to be viable over 100 days post-surgery. Hematoxylin and eosin (H&E) staining showed connections between the transplanted cells and the host spine tissue. Immunohistochemistry showed many Tuj1-, Isl 1/2, and EN1-positive cells and nerve fibers in the transplanted area of the spinal cord after BA Gelfoam ® or DP Gelfoam ® cultures were transplanted to the spine. The spinal cord of mice was injured to effect hind-limb paralysis. Twenty-eight days after transplantation with BA or DP HAP stem cells on Gelfoam ® to the injured area of the spine, the mice recovered normal locomotion.

The dense gas mass fraction in the W51 cloud and its protoclusters

NASA Astrophysics Data System (ADS)

Ginsburg, Adam; Bally, John; Battersby, Cara; Youngblood, Allison; Darling, Jeremy; Rosolowsky, Erik; Arce, Héctor; Lebrón Santos, Mayra E.

2015-01-01

Context. The density structure of molecular clouds determines how they will evolve. Aims: We map the velocity-resolved density structure of the most vigorously star-forming molecular cloud in the Galactic disk, the W51 giant molecular cloud. Methods: We present new 2 cm and 6 cm maps of H2CO, radio recombination lines, and the radio continuum in the W51 star forming complex acquired with Arecibo and the Green Bank Telescope at ~ 50″ resolution. We use H2CO absorption to determine the relative line-of-sight positions of molecular and ionized gas. We measure gas densities using the H2CO densitometer, including continuous measurements of the dense gas mass fraction (DGMF) over the range 104cm-3mass fraction has been measured over a range of densities with a single data set. Results: The DGMF in W51 A is high, f ≳ 70% above n> 104cm-3, while it is low, f< 20%, in W51 B. We did not detect any H2CO emission throughout the W51 GMC; all gas dense enough to emit under normal conditions is in front of bright continuum sources and therefore is seen in absorption instead. Conclusions: (1) The dense gas fraction in the W51 A and B clouds shows that W51 A will continue to form stars vigorously, while star formation has mostly ended in W51 B. The lack of dense, star-forming gas around W51 C indicates that collect-and-collapse is not acting or is inefficient in W51. (2) Ongoing high-mass star formation is correlated with n ≳ 1 × 105cm-3 gas. Gas with n> 104cm-3 is weakly correlated with low and moderate mass star formation, but does not strongly correlate with high-mass star formation. (3) The nondetection of H2CO emission implies that the emission detected in other galaxies, e.g. Arp 220, comes from high-density gas that is not directly affiliated with already-formed massive stars. Either the non-star-forming ISM of these galaxies is very dense, implying the star formation density threshold is higher, or H ii regions

DEMONSTRATION OF NO-VOC/NO-HAP WOOD FURNITURE COATING SYSTEM

EPA Science Inventory

The United States Environmental Protection Agency has contracted with AeroVironment Environmental Services, Inc. and its subcontractor, Adhesives Coating Co., to develop and demonstrate a no-VOC (volatile organic compound)/no-HAP (hazardous air pollutant) wood furniture coating s...

The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein.

PubMed

Fédry, Juliette; Liu, Yanjie; Péhau-Arnaudet, Gérard; Pei, Jimin; Li, Wenhao; Tortorici, M Alejandra; Traincard, François; Meola, Annalisa; Bricogne, Gérard; Grishin, Nick V; Snell, William J; Rey, Félix A; Krey, Thomas

2017-02-23

Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and protists (including important human pathogens like Plasmodium)-suggests that many eukaryotic organisms share a common gamete fusion mechanism. Here, we report combined bioinformatic, biochemical, mutational, and X-ray crystallographic studies on the unicellular alga Chlamydomonas reinhardtii HAP2 that reveal homology to class II viral membrane fusion proteins. We further show that targeting the segment corresponding to the fusion loop by mutagenesis or by antibodies blocks gamete fusion. These results demonstrate that HAP2 is the gamete fusogen and suggest a mechanism of action akin to viral fusion, indicating a way to block Plasmodium transmission and highlighting the impact of virus-cell genetic exchanges on the evolution of eukaryotic life. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

40 CFR Table 3 to Subpart Ffff of... - Emission Limits for Hydrogen Halide and Halogen HAP Emissions or HAP Metals Emissions From...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Emission Limits for Hydrogen Halide and..., Table 3 Table 3 to Subpart FFFF of Part 63—Emission Limits for Hydrogen Halide and Halogen HAP Emissions... limit in the following table that applies to your process vents that contain hydrogen halide and halogen...

40 CFR Table 3 to Subpart Ffff of... - Emission Limits for Hydrogen Halide and Halogen HAP Emissions or HAP Metals Emissions From...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Emission Limits for Hydrogen Halide.... FFFF, Table 3 Table 3 to Subpart FFFF of Part 63—Emission Limits for Hydrogen Halide and Halogen HAP... limit in the following table that applies to your process vents that contain hydrogen halide and halogen...

40 CFR Table 3 to Subpart Ffff of... - Emission Limits for Hydrogen Halide and Halogen HAP Emissions or HAP Metals Emissions From...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Emission Limits for Hydrogen Halide.... FFFF, Table 3 Table 3 to Subpart FFFF of Part 63—Emission Limits for Hydrogen Halide and Halogen HAP... limit in the following table that applies to your process vents that contain hydrogen halide and halogen...

Suggestibility, expectancy, trance state effects, and hypnotic depth: II. Assessment via the PCI-HAP.

PubMed

Pekala, Ronald J; Kumar, V K; Maurer, Ronald; Elliott-Carter, Nancy; Moon, Edward; Mullen, Karen

2010-04-01

This study sought to determine if self-reported hypnotic depth (srHD) could be predicted from the variables of the Phenomenology of Consciousness Inventory - Hypnotic Assessment Procedure (PCI-HAP) (Pekala, 1995a, 1995b; Pekala & Kumar, 2007; Pekala et al., 2010), assessing several of the processes theorized by researchers to be associated with hypnotism: trance (altered state effects), suggestibility, and expectancy. One hundred and eighty participants completed the PCI-HAP. Using regression analyses, srHD scores were predicted from the PCI-HAP pre-hypnotic and post-hypnotic assessment items, and several other variables. The results suggested that the srHD scores were found to be a function of imagoic suggestibility, expectancy (both estimated hypnotic depth and expected therapeutic efficacy), and trance state and eye catalepsy effects; effects that appear to be additive and not (statistically) interactive. The results support the theorizing of many investigators concerning the involvement of the aforementioned component processes with this particular aspect of hypnotism, the self-reported hypnotic depth score.

Crystal structure of the Haemophilus influenzae Hap adhesin reveals an intercellular oligomerization mechanism for bacterial aggregation

PubMed Central

Meng, Guoyu; Spahich, Nicole; Kenjale, Roma; Waksman, Gabriel; St Geme, Joseph W

2011-01-01

Bacterial biofilms are complex microbial communities that are common in nature and are being recognized increasingly as an important determinant of bacterial virulence. However, the structural determinants of bacterial aggregation and eventual biofilm formation have been poorly defined. In Gram-negative bacteria, a major subgroup of extracellular proteins called self-associating autotransporters (SAATs) can mediate cell–cell adhesion and facilitate biofilm formation. In this study, we used the Haemophilus influenzae Hap autotransporter as a prototype SAAT to understand how bacteria associate with each other. The crystal structure of the H. influenzae HapS passenger domain (harbouring the SAAT domain) was determined to 2.2 Å by X-ray crystallography, revealing an unprecedented intercellular oligomerization mechanism for cell–cell interaction. The C-terminal SAAT domain folds into a triangular-prism-like structure that can mediate Hap–Hap dimerization and higher degrees of multimerization through its F1–F2 edge and F2 face. The intercellular multimerization can give rise to massive buried surfaces that are required for overcoming the repulsive force between cells, leading to bacterial cell–cell interaction and formation of complex microcolonies. PMID:21841773

New insights into modeling an organic mass fraction of sea spray aerosol

NASA Astrophysics Data System (ADS)

Meskhidze, N.; Gantt, B.

2010-12-01

As the study of climate change progresses, a need to separate the effects of natural and anthropogenic processes becomes essential in order to correctly forecast the future climate. Due to their massive source regions underlying an atmosphere with low aerosol concentration, marine aerosols derived from sea spray and ocean emitted biogenic volatile organic compounds (BVOCs) are extremely important for the Earth’s radiative budget, regional air quality and biogeochemical cycling of elements. Measurements of freshly-emitted sea spray have revealed that bubble bursting processes, largely responsible for the production of sea salt aerosol, also control sea-to-air transfer of marine organic matter. It has been established that the organic mass fraction of sea spray can be a function of sea-water composition (e.g., concentrations of Chlorophyll-a, [Chl-a], dissolved organic carbon, [DOC], particulate organic carbon, [POC], types of organic carbon, and the amount of surfactants). Current paramaterizations of marine primary organic aerosol emissions use remotely sensed [Chl-a] data as a proxy for oceanic biological activity. However, it has also been shown that the path length, size, and lifetime of bubbles in seawater as well as spatial coverage of seawater surface by streaks or slicks (visible film of a roughly 50 μm thick layer, highly enriched in organics) can have dramatic effect on organic mass fraction of sea spray (OCss). Dynamics of bubble entrainment and the level of microlayer enrichment by organics relative to the underlying bulk water can be controlled by surface wind speed. For bubble entrainment, high winds can increase rising bubble path length and therefore the amount of organics scavenged by the bubble. However, when the surface wind speeds exceed 8 m s-1 breaking of ocean waves can entirely destroy surface organic films and diminish the amount of organics leaving the sea. Despite the probable impact of wind speed, existing parameterizations do not

Health Activities Project (HAP): Heart Fitness and Action Module.

ERIC Educational Resources Information Center

Buller, Dave; And Others

Contained within this Health Activities Project (HAP) learning packet are activities for children in grades 5-8. Design of the activities centers around the idea that students can control their own health and safety. Within the Heart Fitness and Action Module are teacher and student folios describing five activities which involve students in…

The temperate marine phage PhiHAP-1 of Halomonas aquamarina possesses a linear plasmid-like prophage genome.

PubMed

Mobberley, Jennifer M; Authement, R Nathan; Segall, Anca M; Paul, John H

2008-07-01

A myovirus-like temperate phage, PhiHAP-1, was induced with mitomycin C from a Halomonas aquamarina strain isolated from surface waters in the Gulf of Mexico. The induced cultures produced significantly more virus-like particles (VLPs) (3.73 x 10(10) VLP ml(-1)) than control cultures (3.83 x 10(7) VLP ml(-1)) when observed with epifluorescence microscopy. The induced phage was sequenced by using linker-amplified shotgun libraries and contained a genome 39,245 nucleotides in length with a G+C content of 59%. The PhiHAP-1 genome contained 46 putative open reading frames (ORFs), with 76% sharing significant similarity (E value of <10(-3)) at the protein level with other sequences in GenBank. Putative functional gene assignments included small and large terminase subunits, capsid and tail genes, an N6-DNA adenine methyltransferase, and lysogeny-related genes. Although no integrase was found, the PhiHAP-1 genome contained ORFs similar to protelomerase and parA genes found in linear plasmid-like phages with telomeric ends. Southern probing and PCR analysis of host genomic, plasmid, and PhiHAP-1 DNA indicated a lack of integration of the prophage with the host chromosome and a difference in genome arrangement between the prophage and virion forms. The linear plasmid prophage form of PhiHAP-1 begins with the protelomerase gene, presumably due to the activity of the protelomerase, while the induced phage particle has a circularly permuted genome that begins with the terminase genes. The PhiHAP-1 genome shares synteny and gene similarity with coliphage N15 and vibriophages VP882 and VHML, suggesting an evolutionary heritage from an N15-like linear plasmid prophage ancestor.

40 CFR Table 30 to Subpart Uuu of... - Operating Limits for HAP Emissions From Sulfur Recovery Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Operating Limits for HAP Emissions From Sulfur Recovery Units 30 Table 30 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 30 Table 30 to Subpart UUU of Part 63—Operating Limits for HAP Emissions From Sulfur...

40 CFR Table 30 to Subpart Uuu of... - Operating Limits for HAP Emissions From Sulfur Recovery Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Operating Limits for HAP Emissions From Sulfur Recovery Units 30 Table 30 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 30 Table 30 to Subpart UUU of Part 63—Operating Limits for HAP Emissions...

40 CFR Table 36 to Subpart Uuu of... - Work Practice Standards for HAP Emissions From Bypass Lines

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Work Practice Standards for HAP Emissions From Bypass Lines 36 Table 36 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 36 Table 36 to Subpart UUU of Part 63—Work Practice Standards for HAP Emissions From...

40 CFR Table 30 to Subpart Uuu of... - Operating Limits for HAP Emissions From Sulfur Recovery Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Operating Limits for HAP Emissions From Sulfur Recovery Units 30 Table 30 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 30 Table 30 to Subpart UUU of Part 63—Operating Limits for HAP Emissions...

40 CFR Table 36 to Subpart Uuu of... - Work Practice Standards for HAP Emissions From Bypass Lines

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Work Practice Standards for HAP Emissions From Bypass Lines 36 Table 36 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 36 Table 36 to Subpart UUU of Part 63—Work Practice Standards for HAP Emissions From...

40 CFR Table 36 to Subpart Uuu of... - Work Practice Standards for HAP Emissions From Bypass Lines

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Work Practice Standards for HAP Emissions From Bypass Lines 36 Table 36 to Subpart UUU of Part 63 Protection of Environment ENVIRONMENTAL..., Subpt. UUU, Table 36 Table 36 to Subpart UUU of Part 63—Work Practice Standards for HAP Emissions From...

Redirection of the Respiro-Fermentative Flux Distribution in Saccharomyces cerevisiae by Overexpression of the Transcription Factor Hap4p

PubMed Central

Blom, Jolanda; De Mattos, M. Joost Teixeira; Grivell, Leslie A.

2000-01-01

Reduction of aerobic fermentation on sugars by altering the fermentative/oxidative balance is of significant interest for optimization of industrial production of Saccharomyces cerevisiae. Glucose control of oxidative metabolism in baker's yeast is partly mediated through transcriptional regulation of the Hap4p subunit of the Hap2/3/4/5p transcriptional activator complex. To alleviate glucose repression of oxidative metabolism, we constructed a yeast strain with constitutively elevated levels of Hap4p. Genetic analysis of expression levels of glucose-repressed genes and analysis of respiratory capacity showed that Hap4p overexpression (partly) relieves glucose repression of respiration. Analysis of the physiological properties of the Hap4p overproducer in batch cultures in fermentors (aerobic, glucose excess) has shown that the metabolism of this strain is more oxidative than in the wild-type strain, resulting in a significant reduced ethanol production and improvement of growth rate and a 40% gain in biomass yield. Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms. PMID:10788368

Oxygen-Dependent Transcriptional Regulator Hap1p Limits Glucose Uptake by Repressing the Expression of the Major Glucose Transporter Gene RAG1 in Kluyveromyces lactis▿

PubMed Central

Bao, Wei-Guo; Guiard, Bernard; Fang, Zi-An; Donnini, Claudia; Gervais, Michel; Passos, Flavia M. Lopes; Ferrero, Iliana; Fukuhara, Hiroshi; Bolotin-Fukuhara, Monique

2008-01-01

The HAP1 (CYP1) gene product of Saccharomyces cerevisiae is known to regulate the transcription of many genes in response to oxygen availability. This response varies according to yeast species, probably reflecting the specific nature of their oxidative metabolism. It is suspected that a difference in the interaction of Hap1p with its target genes may explain some of the species-related variation in oxygen responses. As opposed to the fermentative S. cerevisiae, Kluyveromyces lactis is an aerobic yeast species which shows different oxygen responses. We examined the role of the HAP1-equivalent gene (KlHAP1) in K. lactis. KlHap1p showed a number of sequence features and some gene targets (such as KlCYC1) in common with its S. cerevisiae counterpart, and KlHAP1 was capable of complementing the hap1 mutation. However, the KlHAP1 disruptant showed temperature-sensitive growth on glucose, especially at low glucose concentrations. At normal temperature, 28°C, the mutant grew well, the colony size being even greater than that of the wild type. The most striking observation was that KlHap1p repressed the expression of the major glucose transporter gene RAG1 and reduced the glucose uptake rate. This suggested an involvement of KlHap1p in the regulation of glycolytic flux through the glucose transport system. The ΔKlhap1 mutant showed an increased ability to produce ethanol during aerobic growth, indicating a possible transformation of its physiological property to Crabtree positivity or partial Crabtree positivity. Dual roles of KlHap1p in activating respiration and repressing fermentation may be seen as a basis of the Crabtree-negative physiology of K. lactis. PMID:18806211

Analysis of consecutively sampled headspace and liquid fractions by gas chromatography/mass spectrometry.

PubMed

Treble, Ronald G; Johnson, Keith E; Xiao, Li; Thompson, Thomas S

2002-07-01

An existing gas chromatograph/mass spectrometer (GC/MS) can be used to analyze gas and liquid fractions from the same system within a few minutes. The technique was applied to (a) separate and identify the gaseous components of the products of cracking an alkane, (b) measure trace levels of acetone in ethyl acetate, (c) determine the relative partial pressures over a binary mixture, and (d) identify nine unknown compounds for the purpose of disposal.

Mass-Dependent and -Independent Fractionation of Mercury Isotopes in Aquatic Systems

NASA Astrophysics Data System (ADS)

Bergquist, B. A.; Joel, B. D.; Jude, D. J.

2008-12-01

Mercury is a globally distributed and highly toxic pollutant. Although Hg is a proven health risk, much of the natural cycle of Hg is not well understood and new approaches are needed to track Hg and the chemical transformations it undergoes in the environment. Recently, we demonstrated that Hg isotopes exhibit two types of isotope fractionation: (1) mass dependent fractionation (MDF) and (2) mass independent fractionation (MIF) of only the odd isotopes (Bergquist and Blum, 2007). The observation of large MIF of Hg isotopes (up to 5 permil) is exciting because only a few other isotopic systems have been documented to display large MIF, the most notable of which are oxygen and sulfur. In both cases, the application of MIF has proven very useful in a variety of fields including cosmochemistry, paleoclimatology, physical chemistry, atmospheric chemistry, and biogeochemistry. Both MDF and MIF isotopic signatures are observed in natural samples, and together they open the door to a new method for tracing Hg pollution and for investigating Hg behavior in the environment. For example, fish record MDF that appears to be related to size and age. Additionally, fish display MIF signatures that are consistent with the photo-reduction of methylmercury (Bergquist and Blum, 2007). If the MDF and MIF in ecosystems can be understood, the signatures in fish could inform us about the sources and processes transforming Hg and why there are differences in the bioaccumulation of Hg in differing ecosystems and populations of fish. This requires sampling of a variety of ecosystems, the sampling of many components of the ecosystems, and the use of other tracers such as carbon and nitrogen isotopes. We have expanded our studies of aquatic ecosystems to include several lakes in North America. Similar to other isotopic systems used to study food web dynamics and structure (i.e., C and N), the MDF of Hg in fish appears to be related to size and age. The MDF recorded in fish likely reflects

HapHop-Physio: a computer game to support cognitive therapies in children.

PubMed

Rico-Olarte, Carolina; López, Diego M; Narváez, Santiago; Farinango, Charic D; Pharow, Peter S

2017-01-01

Care and support of children with physical or mental disabilities are accompanied with serious concerns for parents, families, healthcare institutions, schools, and their communities. Recent studies and technological innovations have demonstrated the feasibility of providing therapy and rehabilitation services to children supported by computer games. The aim of this paper is to present HapHop-Physio, an innovative computer game that combines exercise with fun and learning, developed to support cognitive therapies in children. Conventional software engineering methods such as the Scrum methodology, a functionality test and a related usability test, were part of the comprehensive methodology adapted to develop HapHop-Physio. The game supports visual and auditory attention therapies, as well as visual and auditory memory activities. The game was developed by a multidisciplinary team, which was based on the Hopscotch ® platform provided by Fraunhofer Institute for Digital Media Technology IDMT Institute in Germany, and designed in collaboration with a rehabilitation clinic in Colombia. HapHop-Physio was tested and evaluated to probe its functionality and user satisfaction. The results show the development of an easy-to-use and funny game by a multidisciplinary team using state-of-the-art videogame technologies and software methodologies. Children testing the game concluded that they would like to play again while undergoing rehabilitation therapies.

HAP-PRO USER'S MANUAL (FOR USE WITH VERSION 1.0)

EPA Science Inventory

The primary purpose of the Hazardous Air Pollutant Program (HAP-PRO) is to assist permit engineers in reviewing applications for control of air toxics by calculating the capital and annual costs for 6 volatile organic compound (VOC) and 3 different particulate control devices, i...

CASE STUDIES: LOW-VOC/HAP WOOD FURNITURE COATINGS (PROJECT SUMMARY)

EPA Science Inventory

The report gives results of a study in which wood furniture manufacturing fa-cilities were identified that had converted at least one of their primary coating steps to low-volatile organic compound (VOC)/hazardous air pollut-ant (HAP) wood furniture coatings [high-solids, waterbo...

Unbiased in-depth characterization of CEX fractions from a stressed monoclonal antibody by mass spectrometry.

PubMed

Griaud, François; Denefeld, Blandine; Lang, Manuel; Hensinger, Héloïse; Haberl, Peter; Berg, Matthias

2017-07-01

Characterization of charge-based variants by mass spectrometry (MS) is required for the analytical development of a new biologic entity and its marketing approval by health authorities. However, standard peak-based data analysis approaches are time-consuming and biased toward the detection, identification, and quantification of main variants only. The aim of this study was to characterize in-depth acidic and basic species of a stressed IgG1 monoclonal antibody using comprehensive and unbiased MS data evaluation tools. Fractions collected from cation ion exchange (CEX) chromatography were analyzed as intact, after reduction of disulfide bridges, and after proteolytic cleavage using Lys-C. Data of both intact and reduced samples were evaluated consistently using a time-resolved deconvolution algorithm. Peptide mapping data were processed simultaneously, quantified and compared in a systematic manner for all MS signals and fractions. Differences observed between the fractions were then further characterized and assigned. Time-resolved deconvolution enhanced pattern visualization and data interpretation of main and minor modifications in 3-dimensional maps across CEX fractions. Relative quantification of all MS signals across CEX fractions before peptide assignment enabled the detection of fraction-specific chemical modifications at abundances below 1%. Acidic fractions were shown to be heterogeneous, containing antibody fragments, glycated as well as deamidated forms of the heavy and light chains. In contrast, the basic fractions contained mainly modifications of the C-terminus and pyroglutamate formation at the N-terminus of the heavy chain. Systematic data evaluation was performed to investigate multiple data sets and comprehensively extract main and minor differences between each CEX fraction in an unbiased manner.

Unbiased in-depth characterization of CEX fractions from a stressed monoclonal antibody by mass spectrometry

PubMed Central

Griaud, François; Denefeld, Blandine; Lang, Manuel; Hensinger, Héloïse; Haberl, Peter; Berg, Matthias

2017-01-01

ABSTRACT Characterization of charge-based variants by mass spectrometry (MS) is required for the analytical development of a new biologic entity and its marketing approval by health authorities. However, standard peak-based data analysis approaches are time-consuming and biased toward the detection, identification, and quantification of main variants only. The aim of this study was to characterize in-depth acidic and basic species of a stressed IgG1 monoclonal antibody using comprehensive and unbiased MS data evaluation tools. Fractions collected from cation ion exchange (CEX) chromatography were analyzed as intact, after reduction of disulfide bridges, and after proteolytic cleavage using Lys-C. Data of both intact and reduced samples were evaluated consistently using a time-resolved deconvolution algorithm. Peptide mapping data were processed simultaneously, quantified and compared in a systematic manner for all MS signals and fractions. Differences observed between the fractions were then further characterized and assigned. Time-resolved deconvolution enhanced pattern visualization and data interpretation of main and minor modifications in 3-dimensional maps across CEX fractions. Relative quantification of all MS signals across CEX fractions before peptide assignment enabled the detection of fraction-specific chemical modifications at abundances below 1%. Acidic fractions were shown to be heterogeneous, containing antibody fragments, glycated as well as deamidated forms of the heavy and light chains. In contrast, the basic fractions contained mainly modifications of the C-terminus and pyroglutamate formation at the N-terminus of the heavy chain. Systematic data evaluation was performed to investigate multiple data sets and comprehensively extract main and minor differences between each CEX fraction in an unbiased manner. PMID:28379786

Population differences in the rate of proliferation of international HapMap cell lines.

PubMed

Stark, Amy L; Zhang, Wei; Zhou, Tong; O'Donnell, Peter H; Beiswanger, Christine M; Huang, R Stephanie; Cox, Nancy J; Dolan, M Eileen

2010-12-10

The International HapMap Project is a resource for researchers containing genotype, sequencing, and expression information for EBV-transformed lymphoblastoid cell lines derived from populations across the world. The expansion of the HapMap beyond the four initial populations of Phase 2, referred to as Phase 3, has increased the sample number and ethnic diversity available for investigation. However, differences in the rate of cellular proliferation between the populations can serve as confounders in phenotype-genotype studies using these cell lines. Within the Phase 2 populations, the JPT and CHB cell lines grow faster (p < 0.0001) than the CEU or YRI cell lines. Phase 3 YRI cell lines grow significantly slower than Phase 2 YRI lines (p < 0.0001), with no widespread genetic differences based on common SNPs. In addition, we found significant growth differences between the cell lines in the Phase 2 ASN populations and the Han Chinese from the Denver metropolitan area panel in Phase 3 (p < 0.0001). Therefore, studies that separate HapMap panels into discovery and replication sets must take this into consideration. Copyright © 2010 The American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.

Time-dependent cytokine expression in bone of experimental animals after hydroxyapatite (Hap) implantation

NASA Astrophysics Data System (ADS)

Pilmane, M.; Salms, G.; Salma, I.; Skagers, A.; Locs, J.; Loca, D.; Berzina-Cimdina, L.

2011-06-01

Proinflammatory cytokines mediate bone loss around the implants in patients with peri-implant disease. However, there is no complete data about the expression of cytokines into the bone around the implants. The aim of this work was to investigate the distribution and appearance of inflammatory cytokines and anti-inflammatory proteins in the bone of jaw of experimental rabbits in different time periods after HAp implantation. Material was obtained from 8 rabbits in lower jaw 6 and 8 months after HAp implants were placed. Tissues were processed for immunohistochemical detection of tumor necrosis factor alfa (TNFα), Interleukin 1, 6, 8, 10 (IL-1, IL-6, IL-8, IL-10) and defensin 2. Results demonstrated practically unchanged expression of IL-6 and IL-10 between both - experimental and control side 6 months after implantation, while IL-1 and IL-8 notably increased in control side. IL-1 and IL-10 expression did not change in either the experimental side nor the controle side after 8 months HAP implantation, but IL-6 and IL-8 demonstrated a decrease in the control sites. Only IL-8 was elevated with time in experimental sites, while IL-10 showed individual variations in 2 cases.

Oxygenated fraction and mass of organic aerosol from direct emission and atmospheric processing measured on the R/V Ronald Brown during TEXAQS/GoMACCS 2006

NASA Astrophysics Data System (ADS)

Russell, L. M.; Takahama, S.; Liu, S.; Hawkins, L. N.; Covert, D. S.; Quinn, P. K.; Bates, T. S.

2009-04-01

Submicron particles collected on Teflon filters aboard the R/V Ronald Brown during the Texas Air Quality Study and Gulf of Mexico Atmospheric Composition and Climate Study (TexAQS/GoMACCS) 2006 in and around the port of Houston, Texas, were measured by Fourier transform infrared (FTIR) and X-ray fluorescence for organic functional groups and elemental composition. Organic mass (OM) concentrations (1-25 μg m-3) for ambient particle samples measured by FTIR showed good agreement with measurements made with an aerosol mass spectrometer. The fractions of organic mass identified as alkane and carboxylic acid groups were 47% and 32%, respectively. Three different types of air masses were identified on the basis of the air mass origin and the radon concentration, with significantly higher carboxylic acid group mass fractions in air masses from the north (35%) than the south (29%) or Gulf of Mexico (26%). Positive matrix factorization analysis attributed carboxylic acid fractions of 30-35% to factors with mild or strong correlations (r > 0.5) to elemental signatures of oil combustion and 9-24% to wood smoke, indicating that part of the carboxylic acid fraction of OM was formed by the same sources that controlled the metal emissions, namely the oil and wood combustion activities. The implication is that a substantial part of the measured carboxylic acid contribution was formed independently of traditionally "secondary" processes, which would be affected by atmospheric (both photochemical and meteorological) conditions and other emission sources. The carboxylic acid group fractions in the Gulf of Mexico and south air masses (GAM and SAM, respectively) were largely oil combustion emissions from ships as well as background marine sources, with only limited recent land influences (based on radon concentrations). Alcohol groups accounted for 14% of OM (mostly associated with oil combustion emissions and background sources), and amine groups accounted for 4% of OM in all air

Relative Contribution of P5 and Hap Surface Proteins to Nontypable Haemophilus influenzae Interplay with the Host Upper and Lower Airways

PubMed Central

Viadas, Cristina; Ruiz de los Mozos, Igor; Valle, Jaione; Bengoechea, José Antonio; Garmendia, Junkal

2015-01-01

Nontypable Haemophilus influenzae (NTHi) is a major cause of opportunistic respiratory tract disease, and initiates infection by colonizing the nasopharynx. Bacterial surface proteins play determining roles in the NTHi-airways interplay, but their specific and relative contribution to colonization and infection of the respiratory tract has not been addressed comprehensively. In this study, we focused on the ompP5 and hap genes, present in all H. influenzae genome sequenced isolates, and encoding the P5 and Hap surface proteins, respectively. We employed isogenic single and double mutants of the ompP5 and hap genes generated in the pathogenic strain NTHi375 to evaluate P5 and Hap contribution to biofilm growth under continuous flow, to NTHi adhesion, and invasion/phagocytosis on nasal, pharyngeal, bronchial, alveolar cultured epithelial cells and alveolar macrophages, and to NTHi murine pulmonary infection. We show that P5 is not required for bacterial biofilm growth, but it is involved in NTHi interplay with respiratory cells and in mouse lung infection. Mechanistically, P5NTHi375 is not a ligand for CEACAM1 or α5 integrin receptors. Hap involvement in NTHi375-host interaction was shown to be limited, despite promoting bacterial cell adhesion when expressed in H. influenzae RdKW20. We also show that Hap does not contribute to bacterial biofilm growth, and that its absence partially restores the deficiency in lung infection observed for the ΔompP5 mutant. Altogether, this work frames the relative importance of the P5 and Hap surface proteins in NTHi virulence. PMID:25894755

Windward fraction of the total mass or heat transport for flow past a circular cylinder

NASA Technical Reports Server (NTRS)

Gokoglu, S.; Rosner, D. E.

1983-01-01

The windward fraction of the total mass or heat transport for flow past a cylindrical aerodynamic object was estimated using the available experimental data for the angular distribution of the Nusselt transfer coefficient, Nu(theta, Re). The Re dependence of the windward surface fraction was calculated for the values of Re between 2 and 400,000. The results obtained from polar integrations of data from eight sources indicate that, for Reynolds numbers up to about 2000, more than 70 percent of the total transfer occurs on the windward surface. For the Re values above 100,000, the windward percentage is less than 50 percent.

Effect of Various Sodium Chloride Mass Fractions on Wheat and Rye Bread Using Different Dough Preparation Techniques

PubMed Central

Tańska, Małgorzata; Rotkiewicz, Daniela; Piętak, Andrzej

2016-01-01

Summary This study assessed the selected properties of bread with reduced amount of sodium chloride. The bread was made from white and wholemeal wheat flour and rye flour. The dough was prepared using three techniques: with yeast, natural sourdough or starter sourdough. Sodium chloride was added to the dough at 0, 0.5, 1.0 and 1.5% of the flour mass. The following bread properties were examined in the study: yield and volume of the loaf, moisture content, crumb firmness and porosity, and organoleptic properties. Reducing the mass fraction of added sodium chloride was not found to have considerable effect on bread yield, whereas it had a significant and variable effect on the loaf volume, and crumb firmness and porosity. Organoleptic assessment showed diverse effects of sodium chloride addition on sensory properties of bread, depending on the type of bread and the dough preparation method. Reduced mass fractions of sodium chloride changed the organoleptic properties of bread made with yeast and with starter sourdough to a greater extent than of bread prepared with natural sourdough. PMID:27904407

40 CFR Table 31 to Subpart Uuu of... - Continuous Monitoring Systems for HAP Emissions From Sulfur Recovery Units

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Continuous Monitoring Systems for HAP Emissions From Sulfur Recovery Units 31 Table 31 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 31 Table 31 to Subpart UUU of Part 63—Continuous Monitoring Systems for HAP Emissions...

40 CFR Table 34 to Subpart Uuu of... - Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units 34 Table 34 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 34 Table 34 to Subpart UUU of Part 63—Continuous Compliance With HAP Emission Limits...

40 CFR Table 31 to Subpart Uuu of... - Continuous Monitoring Systems for HAP Emissions From Sulfur Recovery Units

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Continuous Monitoring Systems for HAP Emissions From Sulfur Recovery Units 31 Table 31 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 31 Table 31 to Subpart UUU of Part 63—Continuous Monitoring Systems for HAP Emissions...

40 CFR Table 34 to Subpart Uuu of... - Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units 34 Table 34 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 34 Table 34 to Subpart UUU of Part 63—Continuous Compliance With HAP Emission Limits...

40 CFR 63.5753 - How do I calculate the combined organic HAP content of aluminum wipedown solvents and aluminum...

Code of Federal Regulations, 2013 CFR

2013-07-01

... HAP content of aluminum wipedown solvents and aluminum recreational boat surface coatings? 63.5753...) National Emission Standards for Hazardous Air Pollutants for Boat Manufacturing Standards for Aluminum Recreational Boat Surface Coating Operations § 63.5753 How do I calculate the combined organic HAP content of...

40 CFR Table 2c to Subpart Zzzz of... - Requirements for Existing Compression Ignition Stationary RICE Located at a Major Source of HAP...

Code of Federal Regulations, 2011 CFR

2011-07-01

... Ignition Stationary RICE Located at a Major Source of HAP Emissions and Existing Spark Ignition Stationary RICE ⤠500 HP Located at a Major Source of HAP Emissions 2c Table 2c to Subpart ZZZZ of Part 63... Stationary RICE Located at a Major Source of HAP Emissions and Existing Spark Ignition Stationary RICE ≤ 500...

40 CFR Table 16 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Reforming Units

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Operating Limits for Organic HAP Emissions From Catalytic Reforming Units 16 Table 16 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 16 Table 16 to Subpart UUU of Part 63—Operating Limits for Organic HAP Emissions From...

40 CFR Table 33 to Subpart Uuu of... - Initial Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Initial Compliance With HAP Emission Limits for Sulfur Recovery Units 33 Table 33 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 33 Table 33 to Subpart UUU of Part 63—Initial Compliance With HAP Emission Limits for...

40 CFR Table 16 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Reforming Units

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Operating Limits for Organic HAP Emissions From Catalytic Reforming Units 16 Table 16 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 16 Table 16 to Subpart UUU of Part 63—Operating Limits for Organic HAP Emissions From...

40 CFR Table 9 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Operating Limits for Organic HAP Emissions From Catalytic Cracking Units 9 Table 9 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 9 Table 9 to Subpart UUU of Part 63—Operating Limits for Organic HAP Emissions From...

40 CFR Table 2 to Subpart Uuu of... - Operating Limits for Metal HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Operating Limits for Metal HAP Emissions From Catalytic Cracking Units 2 Table 2 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 2 Table 2 to Subpart UUU of Part 63—Operating Limits for Metal HAP...

40 CFR Table 34 to Subpart Uuu of... - Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units 34 Table 34 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 34 Table 34 to Subpart UUU of Part 63—Continuous Compliance With HAP...

40 CFR Table 9 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Operating Limits for Organic HAP Emissions From Catalytic Cracking Units 9 Table 9 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 9 Table 9 to Subpart UUU of Part 63—Operating Limits for Organic HAP...

40 CFR Table 9 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Operating Limits for Organic HAP Emissions From Catalytic Cracking Units 9 Table 9 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 9 Table 9 to Subpart UUU of Part 63—Operating Limits for Organic HAP Emissions From...

40 CFR Table 9 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Operating Limits for Organic HAP Emissions From Catalytic Cracking Units 9 Table 9 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 9 Table 9 to Subpart UUU of Part 63—Operating Limits for Organic HAP...

40 CFR Table 33 to Subpart Uuu of... - Initial Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Initial Compliance With HAP Emission Limits for Sulfur Recovery Units 33 Table 33 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 33 Table 33 to Subpart UUU of Part 63—Initial Compliance With HAP...

40 CFR Table 16 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Reforming Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Operating Limits for Organic HAP Emissions From Catalytic Reforming Units 16 Table 16 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 16 Table 16 to Subpart UUU of Part 63—Operating Limits for Organic HAP...

40 CFR Table 33 to Subpart Uuu of... - Initial Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Initial Compliance With HAP Emission Limits for Sulfur Recovery Units 33 Table 33 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 33 Table 33 to Subpart UUU of Part 63—Initial Compliance With HAP...

40 CFR Table 33 to Subpart Uuu of... - Initial Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Initial Compliance With HAP Emission Limits for Sulfur Recovery Units 33 Table 33 to Subpart UUU of Part 63 Protection of Environment..., Subpt. UUU, Table 33 Table 33 to Subpart UUU of Part 63—Initial Compliance With HAP Emission Limits for...

40 CFR Table 34 to Subpart Uuu of... - Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units 34 Table 34 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 34 Table 34 to Subpart UUU of Part 63—Continuous Compliance With HAP...

40 CFR Table 33 to Subpart Uuu of... - Initial Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Initial Compliance With HAP Emission Limits for Sulfur Recovery Units 33 Table 33 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 33 Table 33 to Subpart UUU of Part 63—Initial Compliance With HAP...

40 CFR Table 34 to Subpart Uuu of... - Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Continuous Compliance With HAP Emission Limits for Sulfur Recovery Units 34 Table 34 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 34 Table 34 to Subpart UUU of Part 63—Continuous Compliance With HAP...

40 CFR Table 2 to Subpart Uuu of... - Operating Limits for Metal HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Operating Limits for Metal HAP Emissions From Catalytic Cracking Units 2 Table 2 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 2 Table 2 to Subpart UUU of Part 63—Operating Limits for Metal HAP...

40 CFR Table 16 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Reforming Units

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Operating Limits for Organic HAP Emissions From Catalytic Reforming Units 16 Table 16 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 16 Table 16 to Subpart UUU of Part 63—Operating Limits for Organic HAP...

40 CFR Table 9 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Operating Limits for Organic HAP Emissions From Catalytic Cracking Units 9 Table 9 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 9 Table 9 to Subpart UUU of Part 63—Operating Limits for Organic HAP...

40 CFR Table 16 to Subpart Uuu of... - Operating Limits for Organic HAP Emissions From Catalytic Reforming Units

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Operating Limits for Organic HAP Emissions From Catalytic Reforming Units 16 Table 16 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 16 Table 16 to Subpart UUU of Part 63—Operating Limits for Organic HAP...

40 CFR Table 2 to Subpart Uuu of... - Operating Limits for Metal HAP Emissions From Catalytic Cracking Units

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Operating Limits for Metal HAP Emissions From Catalytic Cracking Units 2 Table 2 to Subpart UUU of Part 63 Protection of Environment... Units Pt. 63, Subpt. UUU, Table 2 Table 2 to Subpart UUU of Part 63—Operating Limits for Metal HAP...

40 CFR 63.5753 - How do I calculate the combined organic HAP content of aluminum wipedown solvents and aluminum...

Code of Federal Regulations, 2012 CFR

2012-07-01

... organic HAP content of aluminum wipedown solvents and aluminum recreational boat surface coatings? 63.5753...) National Emission Standards for Hazardous Air Pollutants for Boat Manufacturing Standards for Aluminum Recreational Boat Surface Coating Operations § 63.5753 How do I calculate the combined organic HAP content of...

40 CFR 63.5753 - How do I calculate the combined organic HAP content of aluminum wipedown solvents and aluminum...

Code of Federal Regulations, 2014 CFR

2014-07-01

... organic HAP content of aluminum wipedown solvents and aluminum recreational boat surface coatings? 63.5753...) National Emission Standards for Hazardous Air Pollutants for Boat Manufacturing Standards for Aluminum Recreational Boat Surface Coating Operations § 63.5753 How do I calculate the combined organic HAP content of...

40 CFR 63.4312 - What records must I keep?

Code of Federal Regulations, 2010 CFR

2010-07-01

... based on testing, you must keep the summary sheet of results provided to you by the manufacturer or... and printing materials. If you conducted testing to determine mass fraction of organic HAP of coating... operated within the operating scenarios used to demonstrate initial compliance, documentation that affected...

HapHop-Physio: a computer game to support cognitive therapies in children

PubMed Central

Rico-Olarte, Carolina; López, Diego M; Narváez, Santiago; Farinango, Charic D; Pharow, Peter S

2017-01-01

Background Care and support of children with physical or mental disabilities are accompanied with serious concerns for parents, families, healthcare institutions, schools, and their communities. Recent studies and technological innovations have demonstrated the feasibility of providing therapy and rehabilitation services to children supported by computer games. Objective The aim of this paper is to present HapHop-Physio, an innovative computer game that combines exercise with fun and learning, developed to support cognitive therapies in children. Methods Conventional software engineering methods such as the Scrum methodology, a functionality test and a related usability test, were part of the comprehensive methodology adapted to develop HapHop-Physio. Results The game supports visual and auditory attention therapies, as well as visual and auditory memory activities. The game was developed by a multidisciplinary team, which was based on the Hopscotch® platform provided by Fraunhofer Institute for Digital Media Technology IDMT Institute in Germany, and designed in collaboration with a rehabilitation clinic in Colombia. HapHop-Physio was tested and evaluated to probe its functionality and user satisfaction. Conclusion The results show the development of an easy-to-use and funny game by a multidisciplinary team using state-of-the-art videogame technologies and software methodologies. Children testing the game concluded that they would like to play again while undergoing rehabilitation therapies. PMID:28740440

Mass-dependent and -independent fractionation of Fe isotopes in magnetotactic bacteria

NASA Astrophysics Data System (ADS)

Amor, M.; Busigny, V.; Louvat, P.; Gelabert, A.; Cartigny, P.; Durand-Dubief, M.; Ona-Nguema, G.; Alphandéry, E.; Chebbi, I.; Guyot, F. J.

2016-12-01

Magnetotactic bacteria (MTB) perform biomineralization of intracellular magnetite (Fe3O4) nanoparticles. Although they may be among the oldest microorganisms capable of biomineralization on Earth, identification of their activity in the geological record remains poorly resolved because of the lack of reliable signatures. Here, we determined Fe isotope fractionation by the magnetotactic bacterium Magnetospirillum magneticum strain AMB-1 to better understand Fe cycling in MTB and provide new signatures of the contribution of MTB to iron geochemistry. AMB-1 strain was cultivated with either Fe(III)-quinate or Fe(II)-ascorbate as Fe sources. Iron isotope composition of Fe sources, bacterial growth media after AMB-1 cultures, bacterial lysates (corresponding to AMB-1 cells devoid of magnetite) and magnetite samples were analyzed by MC-ICP-MS after column chromatography. In the two culture conditions, growth media after AMB-1 cultures were enriched in light Fe isotopes relative to Fe sources. Two distinct bacterial Fe reservoirs were characterized in AMB-1: (1) magnetite enriched in the light Fe isotopes by 1.5 to 2.5‰ in δ56Fe relative to Fe sources, and (2) lysate enriched in the heavy Fe isotopes by 0.3 to 0.8‰ relative to Fe sources. More importantly, mass-independent fractionations in odd (57Fe) but not in even isotopes (54Fe, 56Fe and 58Fe) were observed for the first time, highlighting a magnetic isotope effect. Magnetite samples were significantly enriched in 57Fe by 0.23‰ relative to 54Fe, 56Fe and 58Fe. Based on our results, we propose a model for Fe cycling and magnetite biomineralization in AMB-1, and propose to use this specific mass-independent signature of Fe isotopes to evaluate the contribution of MTB to the iron biogeochemistry of recent and ancient environmental samples.

Candida albicans Hap43 Is a Repressor Induced under Low-Iron Conditions and Is Essential for Iron-Responsive Transcriptional Regulation and Virulence ▿

PubMed Central

Hsu, Po-Chen; Yang, Cheng-Yao; Lan, Chung-Yu

2011-01-01

Candida albicans is an opportunistic fungal pathogen that exists as normal flora in healthy human bodies but causes life-threatening infections in immunocompromised patients. In addition to innate and adaptive immunities, hosts also resist microbial infections by developing a mechanism of “natural resistance” that maintains a low level of free iron to restrict the growth of invading pathogens. C. albicans must overcome this iron-deprived environment to cause infections. There are three types of iron-responsive transcriptional regulators in fungi; Aft1/Aft2 activators in yeast, GATA-type repressors in many fungi, and HapX/Php4 in Schizosaccharomyces pombe and Aspergillus species. In this study, we characterized the iron-responsive regulator Hap43, which is the C. albicans homolog of HapX/Php4 and is repressed by the GATA-type repressor Sfu1 under iron-sufficient conditions. We provide evidence that Hap43 is essential for the growth of C. albicans under low-iron conditions and for C. albicans virulence in a mouse model of infection. Hap43 was not required for iron acquisition under low-iron conditions. Instead, it was responsible for repression of genes that encode iron-dependent proteins involved in mitochondrial respiration and iron-sulfur cluster assembly. We also demonstrated that Hap43 executes its function by becoming a transcriptional repressor and accumulating in the nucleus in response to iron deprivation. Finally, we found a connection between Hap43 and the global corepressor Tup1 in low-iron-induced flavinogenesis. Taken together, our data suggest a complex interplay among Hap43, Sfu1, and Tup1 to coordinately regulate iron acquisition, iron utilization, and other iron-responsive metabolic activities. PMID:21131439

Biocorrosion and osteoconductivity of PCL/nHAp composite porous film-based coating of magnesium alloy

NASA Astrophysics Data System (ADS)

Abdal-hay, Abdalla; Amna, Touseef; Lim, Jae Kyoo

2013-04-01

The present study was aimed at designing a novel porous hydroxyapatite/poly(ɛ-caprolactone) (nHAp/PCL) hybrid nanocomposite matrix on a magnesium substrate with high and low porosity. The coated samples were prepared using a dip-coating technique in order to enhance the bioactivity and biocompatibility of the implant and to control the degradation rate of magnesium alloys. The mechanical and biocompatible properties of the coated and uncoated samples were investigated and an in vitro test for corrosion was conducted by electrochemical polarization and measurement of weight loss. The corrosion test results demonstrated that both the pristine PCL and nHAp/PCL composites showed good corrosion resistance in SBF. However, during the extended incubation time, the composite coatings exhibited more uniform and superior resistance to corrosion attack than pristine PCL, and were able to survive severe localized corrosion in physiological solution. Furthermore, the bioactivity of the composite film was determined by the rapid formation of uniform CaP nanoparticles on the sample surfaces during immersion in SBF. The mechanical integrity of the composite coatings displayed better performance (˜34% higher) than the uncoated samples. Finally, our results suggest that the nHAp incorporated with novel PCL composite membranes on magnesium substrates may serve as an excellent 3-D platform for cell attachment, proliferation, migration, and growth in bone tissue. This novel as-synthesized nHAp/PCL membrane on magnesium implants could be used as a potential material for orthopedic applications in the future.

Single-step inline hydroxyapatite enrichment facilitates identification and quantitation of phosphopeptides from mass-limited proteomes with MudPIT

PubMed Central

Fonslow, Bryan R.; Niessen, Sherry M.; Singh, Meha; Wong, Catherine C.; Xu, Tao; Carvalho, Paulo C.; Choi, Jeong; Park, Sung Kyu; Yates, John R.

2012-01-01

Herein we report the characterization and optimization of single-step inline enrichment of phosphopeptides directly from small amounts of whole cell and tissue lysates (100 – 500 μg) using a hydroxyapatite (HAP) microcolumn and Multidimensional Protein Identification Technology (MudPIT). In comparison to a triplicate HILIC-IMAC phosphopeptide enrichment study, ~80% of the phosphopeptides identified using HAP-MudPIT were unique. Similarly, analysis of the consensus phosphorylation motifs between the two enrichment methods illustrates the complementarity of calcium-and iron-based enrichment methods and the higher sensitivity and selectivity of HAP-MudPIT for acidic motifs. We demonstrate how the identification of more multiply phosphorylated peptides from HAP-MudPIT can be used to quantify phosphorylation cooperativity. Through optimization of HAP-MudPIT on a whole cell lysate we routinely achieved identification and quantification of ca. 1000 phosphopeptides from a ~1 hr enrichment and 12 hr MudPIT analysis on small quantities of material. Finally, we applied this optimized method to identify phosphorylation sites from a mass-limited mouse brain region, the amygdala (200 – 500 μg), identifying up to 4000 phosphopeptides per run. PMID:22509746

Anticancer activity of bacteriophage T4 and its mutant HAP1 in mouse experimental tumour models.

PubMed

Dabrowska, Krystyna; Opolski, Adam; Wietrzyk, Joanna; Switala-Jelen, Kinga; Godlewska, Joanna; Boratynski, Janusz; Syper, Danuta; Weber-Dabrowska, Beata; Gorski, Andrzej

2004-01-01

Previously, we have shown the ability of the bacteriophage T4 and its substrain HAP1 (selected for a higher affinity to melanoma cells) to reveal antimetastatic activity in a mouse melanoma model. Here, we investigated the potential phage anticancer activity in primary tumour models. Mice were inoculated subcutaneously with B16 or LLC cells (collected from in vitro culture). Bacteriophages T4 and HAP1 were injected intraperitoneally daily (8 x 10(8)pfu/mouse, except the experiment concerning the dose-dependence). Treatment with purified preparations of bacteriophage T4 resulted in significant reduction of tumour size, the effect being dose-dependent. HAP1 was more effective than T4 and its activity was also dose-dependent. Parallel experiments with non-purified bacteriophage lysates resulted in significant stimulation of tumour growth. These data suggest that purified bacteriophages may inhibit tumour growth, a phenomenon with potentially important clinical implications in oncology.

DEVELOPMENT OF A NO-VOC/NO-HAP WOOD FURNITURE COATINGS SYSTEM

EPA Science Inventory

The report gives results of the development and demonstration of a no-VOC (volatile organic compound)/no-HAP (hazardous air pollutant) wood furniture coating system. The performance characteristics of the new coating system are excellent in terms of adhesion, drying time, gloss, ...

Design of Natural Hydroxyapatite as bio-composite ceramics (HAP): Experimental and Numerical Study

NASA Astrophysics Data System (ADS)