Sample records for hatching

  1. Maternal vibration: an important cue for embryo hatching in a subsocial shield bug.

    PubMed

    Mukai, Hiromi; Hironaka, Mantaro; Tojo, Sumio; Nomakuchi, Shintaro

    2014-01-01

    Hatching care has been reported for many taxonomic groups, from invertebrates to vertebrates. The sophisticated care that occurs around hatching time is expected to have an adaptive function supporting the feeble young. However, details of the characteristics of the adaptive function of hatching care remain unclear. This study investigated the hatching care of the subsocial shield bug, Parastrachia japonensis (Heteroptera: Parastrachiidae) to verify its function. Results show that the P. japonensis mothers vibrated the egg mass intermittently while maintaining an egg-guarding posture. Then embryos started to emerge from their shells synchronously. Unlike such behaviors of closely related species, this vibrating behavior was faint, but lasted more than 6 h. To investigate the effect of this behavior on hatching synchrony and hatching success, we observed the hatching pattern and the hatching rate in control, mother-removed, and two artificial vibration groups. Control broods experienced continuous guarding from the mother. Intermittent artificial vibration broods were exposed to vibrations that matched the temporal pattern of maternal vibration produced by a motor. They showed synchronous hatching patterns and high hatching rates. However, for mother-removed broods, which were isolated from the mother, and when we provided continuous artificial vibration that did not match the temporal pattern of the maternal vibration, embryo hatching was not only asynchronous: some embryos failed to emerge from their shells. These results lead us to infer that hatching care in P. japonensis has two functions: hatching regulation and hatching assistance. Nevertheless, several points of observational and circumstantial evidence clearly contraindicate hatching assistance. A reduction in the hatching rate might result from dependence on maternal hatching care as a strong cue in P. japonensis. We conclude that the hatching care of P. japonensis regulates the hatching pattern and serves as an important cue to induce embryo hatching.

  2. Maternal Vibration: An Important Cue for Embryo Hatching in a Subsocial Shield Bug

    PubMed Central

    Mukai, Hiromi; Hironaka, Mantaro; Tojo, Sumio; Nomakuchi, Shintaro

    2014-01-01

    Hatching care has been reported for many taxonomic groups, from invertebrates to vertebrates. The sophisticated care that occurs around hatching time is expected to have an adaptive function supporting the feeble young. However, details of the characteristics of the adaptive function of hatching care remain unclear. This study investigated the hatching care of the subsocial shield bug, Parastrachia japonensis (Heteroptera: Parastrachiidae) to verify its function. Results show that the P. japonensis mothers vibrated the egg mass intermittently while maintaining an egg-guarding posture. Then embryos started to emerge from their shells synchronously. Unlike such behaviors of closely related species, this vibrating behavior was faint, but lasted more than 6 h. To investigate the effect of this behavior on hatching synchrony and hatching success, we observed the hatching pattern and the hatching rate in control, mother-removed, and two artificial vibration groups. Control broods experienced continuous guarding from the mother. Intermittent artificial vibration broods were exposed to vibrations that matched the temporal pattern of maternal vibration produced by a motor. They showed synchronous hatching patterns and high hatching rates. However, for mother-removed broods, which were isolated from the mother, and when we provided continuous artificial vibration that did not match the temporal pattern of the maternal vibration, embryo hatching was not only asynchronous: some embryos failed to emerge from their shells. These results lead us to infer that hatching care in P. japonensis has two functions: hatching regulation and hatching assistance. Nevertheless, several points of observational and circumstantial evidence clearly contraindicate hatching assistance. A reduction in the hatching rate might result from dependence on maternal hatching care as a strong cue in P. japonensis. We conclude that the hatching care of P. japonensis regulates the hatching pattern and serves as an important cue to induce embryo hatching. PMID:24498224

  3. How embryos escape from danger: the mechanism of rapid, plastic hatching in red-eyed treefrogs.

    PubMed

    Cohen, Kristina L; Seid, Marc A; Warkentin, Karen M

    2016-06-15

    Environmentally cued hatching allows embryos to escape dangers and exploit new opportunities. Such adaptive responses require a flexibly regulated hatching mechanism sufficiently fast to meet relevant challenges. Anurans show widespread, diverse cued hatching responses, but their described hatching mechanisms are slow, and regulation of timing is unknown. Arboreal embryos of red-eyed treefrogs, Agalychnis callidryas, escape from snake attacks and other threats by very rapid premature hatching. We used videography, manipulation of hatching embryos and electron microscopy to investigate their hatching mechanism. High-speed video revealed three stages of the hatching process: pre-rupture shaking and gaping, vitelline membrane rupture near the snout, and muscular thrashing to exit through the hole. Hatching took 6.5-49 s. We hypothesized membrane rupture to be enzymatic, with hatching enzyme released from the snout during shaking. To test this, we displaced hatching embryos to move their snout from its location during shaking. The membrane ruptured at the original snout position and embryos became trapped in collapsed capsules; they either moved repeatedly to relocate the hole or shook again and made a second hole to exit. Electron microscopy revealed that hatching glands are densely concentrated on the snout and absent elsewhere. They are full of vesicles in embryos and release most of their contents rapidly at hatching. Agalychnis callidryas' hatching mechanism contrasts with the slow process described in anurans to date and exemplifies one way in which embryos can achieve rapid, flexibly timed hatching to escape from acute threats. Other amphibians with cued hatching may also have novel hatching mechanisms. © 2016. Published by The Company of Biologists Ltd.

  4. Incubation and hatch management: consequences for bone mineralization in Cobb 500 meat chickens.

    PubMed

    Muir, W I; Groves, P J

    2018-04-01

    From ~35 days of age fast growing meat chickens spend extended periods sitting or lying and less time standing. In a fast-feathering parent line lower early incubation temperatures which delayed chick hatch time, improved bone ash and extended their standing time. This incubation study assessed the consequences of incubation temperatures, hatch time and chick management at hatch/take off on femoral bone ash (BA) in Cobb 500 meat chickens. Embryos were incubated under either Control (between 37.8°C and 38.2°C egg shell temperature (EST)) or a Slow start (from 37.2°C at sett (the start of incubation), reaching 37.8°C EST at day 13 incubation), temperatures. Hatched chicks were identified at 492 h (20.5 days of incubation - classified as early (E)) or, between >492 and ⩽516 h (>20.5 and ⩽21.5 days of incubation - classified as late (L)), from setting. The E hatch chicks were allocated across three post-hatch treatments; treatment 1: E hatch chicks that were sampled E at 492 h from setting; treatment 2: E hatch chicks that were fed for a further 24 h in a floorpen before being sampled L at 516 h from setting; treatment 3: E hatch chicks that spent a further 24 h in the incubator before being sampled L at 516 h from setting. All L hatch chicks formed one treatment group which was sampled L at 516 h (i.e. L hatch chicks sampled L). It is not possible to sample L hatching chicks E hence this treatment is absent from the experimental design. Slow start incubation resulted in a higher total hatch percentage with a greater proportion of chicks hatching L, compared with the Control incubation. The L hatching chicks had significantly higher BA than the E hatching chicks. Of the E hatching chicks, those sampled both E and L had significantly lower BA than E hatching chicks fed for 24 h before L sampling. The E hatch, fed and sampled L chicks had the numerically highest BA, which was not significantly different from the BA of the L hatching chicks sampled L These results demonstrate that BA at hatch can be improved, either by extending the incubation period through a Slow start incubation profile, inducing L hatch, or alternatively, via the prompt provision of feed to E hatching chicks.

  5. Efficacy of catheter ablation of atrial fibrillation beyond HATCH score.

    PubMed

    Tang, Ri-Bo; Dong, Jian-Zeng; Long, De-Yong; Yu, Rong-Hui; Ning, Man; Jiang, Chen-Xi; Sang, Cai-Hua; Liu, Xiao-Hui; Ma, Chang-Sheng

    2012-10-01

    HATCH score is an established predictor of progression from paroxysmal to persistent atrial fibrillation (AF). The purpose of this study was to determine if HATCH score could predict recurrence after catheter ablation of AF. The data of 488 consecutive paroxysmal AF patients who underwent an index circumferential pulmonary veins (PV) ablation were retrospectively analyzed. Of these patients, 250 (51.2%) patients had HATCH score = 0, 185 (37.9%) patients had HATCH score = 1, and 53 (10.9%) patients had HATCH score ≥ 2 (28 patients had HATCH score = 2, 23 patients had HATCH score = 3, and 2 patients had HATCH score = 4). The patients with HATCH score ≥ 2 had significantly larger left atrium size, the largest left ventricular end systolic diameter, and the lowest ejection fraction. After a mean follow-up of (823 ± 532) days, the recurrence rates were 36.4%, 37.8% and 28.3% from the HATCH score = 0, HATCH score = 1 to HATCH score ≥ 2 categories (P = 0.498). Univariate analysis revealed that left atrium size, body mass index, and failure of PV isolation were predictors of AF recurrence. After adjustment for body mass index, left atrial size and PV isolation, the HATCH score was not an independent predictor of recurrence (HR = 0.92, 95% confidence interval = 0.76 - 1.12, P = 0.406) in multivariate analysis. HATCH score has no value in prediction of AF recurrence after catheter ablation.

  6. Spread of hatch and delayed feed access affect post hatch performance of female broiler chicks up to day 5.

    PubMed

    Wang, Y; Li, Y; Willems, E; Willemsen, H; Franssens, L; Koppenol, A; Guo, X; Tona, K; Decuypere, E; Buyse, J; Everaert, N

    2014-04-01

    It is not rare that newly hatched chicks remain without feed for about 24 to 48 h before they are placed on farms due to a series of logistic operations. Furthermore, the spread in hatching time can also mount up to 30 to 48 h for late v. early hatchers. In other words, the practice is a complex combination of spread of hatch and delayed feed access. The present study was aimed to investigate the combined effects of hatching time with a delay in feed access of 48 h, starting from their hatch-time (biological age). When chicks had access to feed immediately after hatch, late hatchers had a higher feed intake and relative growth rate up to day 5 compared with their early hatched counterparts. Feed deprivation during the first 48 h resulted in retarded early growth rate, which was further aggravated by an impaired feed intake after refeeding. In addition, the differential effects of hatching time on relative growth rate and feed intake observed in immediately fed chicks were eliminated by the 48 h feed delay. The yolk utilization after hatch was faster for the late hatchers up to biological day 2 regardless of the feeding treatments. Hatching muscle glycogen content was higher in the late hatchers compared with that of their early counterparts at hatch and at biological day 2 independent of feeding treatment. Moreover, the liver glycogen content of the late hatchers was also higher at hatch. For the immediately fed chicks, the proportional breast muscle weight of the late hatchers was higher at biological day 2 and 5. For the starved chicks, on the other hand, this effect was only observed after they had access to feed (biological day 5). The different plasma T3 levels at hatch may have contributed to the different post hatch performance. It is concluded that the spread of hatch influenced post hatch performance, especially appetite and growth at least until day 5. Moreover, the delay in feed access interacted with the hatching time and caused adverse effects on the post hatch performance.

  7. The location of “8”-shaped hatching influences inner cell mass formation in mouse blastocysts

    PubMed Central

    Takahashi, Kazumasa; Goto, Mayumi; Anzai, Mibuki; Ono, Natsuki; Shirasawa, Hiromitsu; Sato, Wataru; Miura, Hiroshi; Sato, Naoki; Sato, Akira; Kumazawa, Yukiyo; Terada, Yukihiro

    2017-01-01

    The hatching of a blastocyst where the blastocyst portions on the inside and the outside of the zona pellucida feature a figure-of-eight shape is termed “8”-shaped hatching; this type of hatching has been reported to affect the proper presentation of the inner cell mass (ICM) in both human and mouse embryos. Here, our aim was to investigate the factors that affect ICM presentation during “8”-shaped hatching. We performed IVF by using B6D2F1 female mice and ICR male mice, and used the 104 captured blastocysts. Embryos were maintained in KSOM at 37°C in a 5% CO2, 5% O2, and 90% N2 environment, and their growth behavior was monitored individually and continuously using time-lapse cinematography. At 120 h after insemination, embryos were immunostained and examined under a confocal microscope. We used the hatching form to identify “8”-shaped hatching, and we classified the “8”-shaped-hatching blastocysts into two groups, one in which the hatching site was near the ICM center, and the other in which the hatching site was far from the ICM center. We measured each group for ICM size and the number of Oct3/4-positive cells. Of the 95 hatching or hatched embryos, 74 were “8”-shaped-hatching blastocysts, and in these embryos, the ICM was significantly wider when the hatching site was near the ICM than when the hatching site was far from the ICM (P = 0.0091). Moreover, in the “8”-shaped-hatching blastocysts in which the ICM was included in the blastocyst portion outside the zona pellucida―the portion defined as the “outside blastocyst”―after the collapse of this outside blastocyst, the ICM adhered to the trophectoderm of the outside blastocyst, opposite the hatching site. Our results indicate that in “8”-shaped-hatching blastocysts, the hatching site and the collapse of outside blastocyst affect ICM formation. Thus, the assessment of “8”-shaped hatching behaviors could yield indices for accurately evaluating embryo quality. PMID:28384351

  8. Effects of mixing eggs of different initial incubation time on the hatching pattern, chick embryonic development and post-hatch performance.

    PubMed

    Zhong, Zhentao; Yu, Yue; Jin, Shufang; Pan, Jinming

    2018-01-01

    The hatch window that varies from 24 to 48 h is known to influence post-hatch performance of chicks. A narrow hatch window is needed for commercial poultry industry to acquire a high level of uniformity of chick quality. Hatching synchronization observed in avian species presents possibilities in altering hatch window in artificial incubation. Layer eggs which were laid on the same day by a single breeder flock and stored for no more than two days started incubation 12 h apart to obtain developmental distinction. The eggs of different initial incubation time were mixed as rows adjacent to rows on day 12 of incubation. During the hatching period (since day 18), hatching time of individual eggs and hatch window were obtained by video recordings. Embryonic development (day 18 and 20) and post-hatch performance up to day 7 were measured. The manipulation of mixing eggs of different initial incubation time shortened the hatch window of late incubated eggs in the manipulated group by delaying the onset of hatching process, and improved the hatchability. Compared to the control groups, chick embryos or chicks in the egg redistribution group showed no significant difference in embryonic development and post-hatch performance up to day 7. We have demonstrated that eggs that were incubated with advanced eggs performed a narrow spread of hatch with higher hatchability, normal embryonic development as well as unaffected chick quality. This specific manipulation is applicable in industrial poultry production to shorten hatch window and improve the uniformity of chick quality.

  9. Hatch assembly

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Marshall, J.R.; Hardin, R.T. Jr.

    1987-07-07

    This patent describes a nuclear reactor installation including means defining a fuel handling area and means defining a containment area separated from the fuel handling area and including a refuelling cavity; the improvement comprising: (a) a fuel transfer tube connecting the refuelling cavity with the fuel handling area; the fuel transfer tube having a first end in the fuel handling area and a second end in the refueling cavity; (b) valve means for opening and closing the first end; and (c) a hatch assembly mounted on the second end; the hatch assembly including (1) a hatch ring affixed to themore » fuel transfer tube at the second end the hatch ring has an integral annular seat surrounded by the hatch ring and defines a hatch opening in the second end of the fuel transfer tube; (2) a hatch cover adapts to be positioned on the annular seat for covering the hatch opening; (3) latching units are supported on the hatch ring about the hatch opening, each latching unit.« less

  10. EGG-HATCHING MECHANISM OF HUMAN LIVER FLUKE, OPISTHORCHIS VIVERRINI: A ROLE FOR LEUCINE AMINOPEPTIDASES FROM THE SNAIL HOST, BITHYNIA SIAMENSIS GONIOMPHALOS.

    PubMed

    Tesana, Smarn; Khampoosa, P; Piratae, S; Prasopdee, S; Sripanidkulchai, B

    2018-05-08

    The human liver fluke Opisthorchis viverrini (Platyhelminthes, Trematoda, Digenea) uses snails of the genus Bithynia as first intermediate host. Peculiarly among trematodes, the eggs of O. viverrini hatch within the digestive tract of its snail host. It remains uncertain whether hatching in this species is mediated through mechanical fracture of the eggshell or by digestion with specific digestive enzymes. This study aimed to characterize enzymes with specific inhibitor and factors involved in the hatching activity of O. viverrini eggs. For measuring egg hatching in vivo, 50 O. viverrini mature eggs were fed to individual Bithynia siamensis goniomphalos snail at various temperature conditions for 24 hrs. Ex vivo, mature eggs were incubated with crude snail extract and commercial leucine aminopeptidase (LAP). Egg-hatching of O. viverrini was temperature dependent, with optimal hatching occurring at 24-28 C, with a peak of hatching of 93.54% in vivo and 30.55% ex vivo occurring at these temperatures. Ex vivo hatching rates increased to 45.87% under anaerobic conditions at 28 C. Some 22.70% and 16.21% of heat-killed eggs also hatched within the snail digestive tract and snail extract, respectively, indicating that host molecules are involved in the hatching response. Most eggs hatch in the anterior regions of the digestive tract. Hatching was completely inhibited in the presence of bestatin, an inhibitor of leucine aminopeptidase (LAP), but not in the presence of phosphatase inhibitors. Bestatin inhibition of hatching was reversible. Finally, egg hatching could be induced by addition of a porcine LAP. The results indicate that this digenean utilizes both LAP of the snail host and movement of miracidia for hatching.

  11. [Predictive value of HATCH score on atrial fibrillation recurrence post radiofrequency catheter ablation].

    PubMed

    Miao, Dan-dan; Zang, Xiao-biao; Zhang, Shu-long; Gao, Lian-jun; Xia, Yun-long; Yin, Xiao-meng; Chang, Dong; Dong, Ying-xue; Yang, Yan-zong

    2012-10-01

    To determine the predictive value of HATCH score on recurrence of atrial fibrillation (AF) after radiofrequency catheter ablation (RFCA). The data of 123 consecutive AF patients (74 paroxysmal and 49 persistent AF) who underwent RFCA between April 2009 and December 2010 in our department were retrospectively analyzed. Of theses patients, 65 (52.9%) patients had HATCH score = 0, 41 (33.3%) patients had HATCH score = 1, and 17 (13.8%) patients had HATCH score ≥ 2 (HATCH = 2 in 11 patients, HATCH = 3 in 5 patients, HATCH = 4 in 1 patient). The recurrence was defined as atrial tachyarrhythmia lasting more than 30 seconds after 3 months post RFCA. The patients were divided into recurrence group and no recurrence group. Relationship between HATCH score and recurrence was observed. There were 43 cases in recurrence group and 80 cases in no recurrence group. After 12 months follow-up, HATCH score was significant higher in recurrence group than in non-recurrence group [(0.91 ± 0.94) score vs. (0.53 ± 0.80) score, P < 0.05]. The ratio of patients with HATCH ≥ 2 in recurrence group was higher than in non-recurrence group [23.3% (10/43) vs. 8.8% (7/80), P < 0.01]. The sensitivity and specificity of HATCH ≥ 2 to define the risk of recurrence was 25.0%, 92.4% respectively. Cumulative non-recurrence rate of patients with HATCH score ≥ 2 was lower than patients with HATCH score = 0 and 1 (P < 0.05). Higher HATCH score is associated with increased risk of AF recurrence post RFCA.

  12. A comparison of artificial incubation and natural incubation hatching success of gopher tortoise (Gopherus polyphemus) eggs in southern Mississippi

    USGS Publications Warehouse

    Noel, Krista M.; Qualls, Carl P.; Ennen, Joshua R.

    2012-01-01

    Recent studies have found that Gopher Tortoise, Gopherus polyphemus, populations in southern Mississippi exhibit low recruitment, due in part to very low hatching success of their eggs. We sought to determine if the cause(s) of this low hatching success was related to egg quality (intrinsic factors), unsuitability of the nest environment (extrinsic factors), or a combination of the two. In 2003, hatching success was monitored simultaneously for eggs from the same clutches that were incubated in the laboratory and left to incubate in nests. A subset of randomly chosen eggs from each clutch was incubated in the laboratory under physical conditions that were known to be conducive to successful hatching to estimate the proportion of eggs that were capable of hatching in a controlled setting. Hatching success in the laboratory was compared with that of eggs incubated in natural nests to estimate the proportion of eggs that failed to hatch presumably from extrinsic factors. Laboratory hatching success was 58.8%, suggesting that roughly 40% of the eggs were intrinsically incapable of hatching even when incubated under controlled conditions. Hatching success in natural nests, 16.7%, was significantly lower than hatching success in the laboratory, suggesting that approximately 42.1% of eggs were capable of hatching but failed to hatch due to some extrinsic aspect(s) of the nest environment. Thus, the low hatching success of Gopher Tortoise eggs in southern Mississippi appears to be attributable to a combination of intrinsic (egg quality) and extrinsic (nest environment) factors.

  13. The influence of encapsulated embryos on the timing of hatching in the brooding gastropod Crepipatella dilatata

    NASA Astrophysics Data System (ADS)

    Andrade-Villagrán, P. V.; Baria, K. S.; Montory, J. A.; Pechenik, J. A.; Chaparro, O. R.

    2018-01-01

    Encapsulated embryos are generally thought to play an active role in escaping from egg capsules or egg masses. However, for species that brood their egg capsules, the factors controlling the timing of hatching are largely unclear, particularly the degree to which hatching is controlled by the embryos rather than by the mother, and the degree to which the hatching of one egg capsule influences the hatching of sister egg capsules within the same egg mass. We studied aspects of hatching using the direct-developing gastropod Crepipatella dilatata, which includes nurse eggs in its egg capsules and broods clusters of egg capsules for at least several weeks before metamorphosed juveniles are released. Isolated egg capsules were able to hatch successfully, in the absence of the mother. Moreover, the hatching of one capsule did not cause adjacent sister capsules to hatch. Hatched and un-hatched sister egg capsules from the same egg mass differed significantly in the number of metamorphosed juveniles, average shell size, offspring biomass (juveniles + veliger larvae), and the number of nurse eggs remaining per egg capsule. Differences in when egg capsules hatched within a single egg mass were not explained by differences in egg capsule age. Hatching occurred only after most nurse eggs had been ingested, most offspring had metamorphosed into juveniles, and juveniles had reached a mean shell length > 1.36 mm. Whether the mother has any role to play in coordinating the hatching process or juvenile release remains to be determined.

  14. 50 CFR Figure 1 to Subpart I of... - Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional.... I, Fig. 1 Figure 1 to Subpart I of Part 660—Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional Catalina...

  15. 50 CFR Figure 1 to Subpart I of... - Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional.... I, Fig. 1 Figure 1 to Subpart I of Part 660—Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional Catalina...

  16. 50 CFR Figure 1 to Subpart I of... - Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional.... I, Fig. 1 Figure 1 to Subpart I of Part 660—Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional Catalina...

  17. 50 CFR Figure 1 to Subpart I of... - Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional.... I, Fig. 1 Figure 1 to Subpart I of Part 660—Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional Catalina...

  18. Standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors Aedes aegypti and Aedes albopictus (Diptera: Culicidae) - II - Egg storage and hatching.

    PubMed

    Zheng, Min-Lin; Zhang, Dong-Jing; Damiens, David D; Lees, Rosemary Susan; Gilles, Jeremie R L

    2015-06-26

    Management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a Sterile Insect Technique component. The efficiency of different hatching media and effectiveness of long term storage methods are presented here. The effect on hatch rate of storage duration and three hatching media was analysed: deionized water, boiled deionized water and a bacterial broth, using Two-way ANOVA and Post hoc Tukey tests, and the Pearson correlation coefficient was used to find the effect on the proportion of collapsed eggs. Two long term storage methods were also tested: conventional storage (egg paper strips stored in zip lock bags within a sealed plastic box), and water storage (egg papers in a covered plastic cup with deionized water). Regression analyses were used to find the effect of water storage and storage duration on hatch rate. Both species hatched most efficiently in bacterial broth. Few eggs hatched in deionized water, and pre-boiling the water increased the hatch rate of Ae. aegypti, but not Ae. albopictus. A hatch rate greater than 80% was obtained after 10 weeks of conventional storage in Ae. aegypti and 11 weeks in Ae. albopictus. After this period, hatching decreased dramatically; no eggs hatched after 24 weeks. Storing eggs in water produced an 85% hatch rate after 5 months in both species. A small but significant proportion of eggs hatched in the water, probably due to combined effects of natural deoxygenation of the water over time and the natural instalment hatching typical of the species. The demonstrated efficiency of the bacterial broth hatching medium for both Ae. albopictus and Ae. aegypti facilitates mass production of these two important vector species in the same facility, with use of a common hatching medium reducing cost and operational complexity. Similarly the increased hatch rate of eggs stored in water would allow greater flexibility of egg management in a large programme over the medium term, particularly if oxygenation of the water by bubbling oxygen through the storage tray could be applied to prevent hatching during storage.

  19. Consequences of Hatch Phenology on Stages of Fish Recruitment.

    PubMed

    Bogner, David M; Kaemingk, Mark A; Wuellner, Melissa R

    2016-01-01

    Little is known about how hatch phenology (e.g., the start, peak, and duration of hatching) could influence subsequent recruitment of freshwater fishes into a population. We used two commonly sympatric fish species that exhibit different hatching phenologies to examine recruitment across multiple life stages. Nine yellow perch (Perca flavescens) and bluegill (Lepomis macrochirus) annual cohorts were sampled from 2004 through 2013 across larval, age-0, age-1, and age-2 life stages in a Nebraska (U.S.A.) Sandhill lake. Yellow perch hatched earlier in the season and displayed a more truncated hatch duration compared to bluegill. The timing of hatch influenced recruitment dynamics for both species but important hatching metrics were not similar between species across life stages. A longer hatch duration resulted in greater larval yellow perch abundance but greater age-1 bluegill abundance. In contrast, bluegill larval and age-0 abundances were greater during years when hatching duration was shorter and commenced earlier, whereas age-0 yellow perch abundance was greater when hatching occurred earlier. As a result of hatch phenology, yellow perch recruitment variability was minimized sooner (age-0 life stage) than bluegill (age-1 life stage). Collectively, hatch phenology influenced recruitment dynamics across multiple life stages but was unique for each species. Understanding the complexities of when progeny enter an environment and how this influences eventual recruitment into a population will be critical in the face of ongoing climate change.

  20. 46 CFR 108.145 - Hatches and tonnage openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Hatches and tonnage openings. 108.145 Section 108.145... AND EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.145 Hatches and tonnage openings. Each hatch, except a hatch between storage spaces and each tonnage opening closure, must be made...

  1. 46 CFR 108.145 - Hatches and tonnage openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Hatches and tonnage openings. 108.145 Section 108.145... AND EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.145 Hatches and tonnage openings. Each hatch, except a hatch between storage spaces and each tonnage opening closure, must be made...

  2. 9 CFR 147.22 - Hatching egg sanitation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Hatching egg sanitation. 147.22... Procedures § 147.22 Hatching egg sanitation. Hatching eggs should be collected from the nests at frequent... collecting the nest eggs for hatching. Egg handlers should thoroughly wash their hands with soap and water...

  3. 46 CFR 108.145 - Hatches and tonnage openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Hatches and tonnage openings. 108.145 Section 108.145... AND EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.145 Hatches and tonnage openings. Each hatch, except a hatch between storage spaces and each tonnage opening closure, must be made...

  4. 9 CFR 147.22 - Hatching egg sanitation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Hatching egg sanitation. 147.22... Procedures § 147.22 Hatching egg sanitation. Hatching eggs should be collected from the nests at frequent... collecting the nest eggs for hatching. Egg handlers should thoroughly wash their hands with soap and water...

  5. 46 CFR 108.145 - Hatches and tonnage openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Hatches and tonnage openings. 108.145 Section 108.145... AND EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.145 Hatches and tonnage openings. Each hatch, except a hatch between storage spaces and each tonnage opening closure, must be made...

  6. 9 CFR 147.22 - Hatching egg sanitation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Hatching egg sanitation. 147.22... Procedures § 147.22 Hatching egg sanitation. Hatching eggs should be collected from the nests at frequent... collecting the nest eggs for hatching. Egg handlers should thoroughly wash their hands with soap and water...

  7. 46 CFR 108.145 - Hatches and tonnage openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Hatches and tonnage openings. 108.145 Section 108.145... AND EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.145 Hatches and tonnage openings. Each hatch, except a hatch between storage spaces and each tonnage opening closure, must be made...

  8. Comparison of the Hatch of Lymantria dispar (Lepidoptera: Lymantriidae) Eggs from Russia and the United States After Exposure to Different Temperatures and Durations of Low Temperature

    Treesearch

    Melody A. Keena

    1996-01-01

    Comparisons are made of the effects of temperature and duration of low temperature on egg hatch of North American and Russian gypsy moth, Lymantria dispar), under controlled laboratory conditions. Percentage of hatch of embryonated eggs, days to 1st hatch after incubation at warm temperature and temperal distribution of hatch are used to compare hatch of different...

  9. Monitoring the hatch time of individual chicken embryos.

    PubMed

    Romanini, C E B; Exadaktylos, V; Tong, Q; McGonnel, I; Demmers, T G M; Bergoug, H; Eterradossi, N; Roulston, N; Garain, P; Bahr, C; Berckmans, D

    2013-02-01

    This study investigated variations in eggshell temperature (T(egg)) during the hatching process of broiler eggs. Temperature sensors monitored embryo temperature by registering T(egg) every minute. Measurements carried out on a sample of 40 focal eggs revealed temperature drops between 2 to 6°C during the last 3 d of incubation. Video cameras recorded the hatching process and served as the gold standard reference for manually labeling the hatch times of chicks. Comparison between T(egg) drops and the hatch time of individuals revealed a time synchronization with 99% correlation coefficient and an absolute average time difference up to 25 min. Our findings suggest that attaching temperature sensors to eggshells is a precise tool for monitoring the hatch time of individual chicks. Individual hatch monitoring registers the biological age of chicks and facilitates an accurate and reliable means to count hatching results and manage the hatch window.

  10. Glassfrog embryos hatch early after parental desertion.

    PubMed

    Delia, Jesse R J; Ramírez-Bautista, Aurelio; Summers, Kyle

    2014-06-22

    Both parental care and hatching plasticity can improve embryo survival. Research has found that parents can alter hatching time owing to a direct effect of care on embryogenesis or via forms of care that cue the hatching process. Because parental care alters conditions critical for offspring development, hatching plasticity could allow embryos to exploit variation in parental behaviour. However, this interaction of parental care and hatching plasticity remains largely unexplored. We tested the hypothesis that embryos hatch early to cope with paternal abandonment in the glassfrog Hyalinobatrachium fleischmanni (Centrolenidae). We conducted male-removal experiments in a wild population, and examined embryos' response to conditions with and without fathers. Embryos hatched early when abandoned, but extended development in the egg stage when fathers continued care. Paternal care had no effect on developmental rate. Rather, hatching plasticity was due to embryos actively hatching at different developmental stages, probably in response to deteriorating conditions without fathers. Our experimental results are supported by a significant correlation between the natural timing of abandonment and hatching in an unmanipulated population. This study demonstrates that embryos can respond to conditions resulting from parental abandonment, and provides insights into how variation in care can affect selection on egg-stage adaptations.

  11. Glassfrog embryos hatch early after parental desertion

    PubMed Central

    Delia, Jesse R. J.; Ramírez-Bautista, Aurelio; Summers, Kyle

    2014-01-01

    Both parental care and hatching plasticity can improve embryo survival. Research has found that parents can alter hatching time owing to a direct effect of care on embryogenesis or via forms of care that cue the hatching process. Because parental care alters conditions critical for offspring development, hatching plasticity could allow embryos to exploit variation in parental behaviour. However, this interaction of parental care and hatching plasticity remains largely unexplored. We tested the hypothesis that embryos hatch early to cope with paternal abandonment in the glassfrog Hyalinobatrachium fleischmanni (Centrolenidae). We conducted male-removal experiments in a wild population, and examined embryos' response to conditions with and without fathers. Embryos hatched early when abandoned, but extended development in the egg stage when fathers continued care. Paternal care had no effect on developmental rate. Rather, hatching plasticity was due to embryos actively hatching at different developmental stages, probably in response to deteriorating conditions without fathers. Our experimental results are supported by a significant correlation between the natural timing of abandonment and hatching in an unmanipulated population. This study demonstrates that embryos can respond to conditions resulting from parental abandonment, and provides insights into how variation in care can affect selection on egg-stage adaptations. PMID:24789892

  12. Egg storage duration and hatch window affect gene expression of nutrient transporters and intestine morphological parameters of early hatched broiler chicks.

    PubMed

    Yalcin, S; Gursel, I; Bilgen, G; Izzetoglu, G T; Horuluoglu, B H; Gucluer, G

    2016-05-01

    In recent years, researchers have given emphasis on the differences in physiological parameters between early and late hatched chicks within a hatch window. Considering the importance of intestine development in newly hatched chicks, however, changes in gene expression of nutrient transporters in the jejunum of early hatched chicks within a hatch window have not been studied yet. This study was conducted to determine the effects of egg storage duration before incubation and hatch window on intestinal development and expression of PepT1 (H+-dependent peptide transporter) and SGLT1 (sodium-glucose co-transporter) genes in the jejunum of early hatched broiler chicks within a 30 h of hatch window. A total of 1218 eggs obtained from 38-week-old Ross 308 broiler breeder flocks were stored for 3 (ES3) or 14 days (ES14) and incubated at the same conditions. Eggs were checked between 475 and 480 h of incubation and 40 chicks from each egg storage duration were weighed; chick length and rectal temperature were measured. The chicks were sampled to evaluate morphological parameters and PepT1 and SGLT1 expression. The remaining chicks that hatched between 475 and 480 h were placed back in the incubator and the same measurements were conducted with those chicks at the end of hatch window at 510 h of incubation. Chick length, chick dry matter content, rectal temperature and weight of small intestine segments increased, whereas chick weight decreased during the hatch window. The increase in the jejunum length and villus width and area during the hatch window were higher for ES3 than ES14 chicks. PepT1 expression was higher for ES3 chicks compared with ES14. There was a 10.2 and 17.6-fold increase in PepT1 and SGLT1 expression of ES3 chicks at the end of hatch window, whereas it was only 2.3 and 3.3-fold, respectively, for ES14 chicks. These results suggested that egg storage duration affected development of early hatched chicks during 30 h of hatch window. It can be concluded that the ES14 chicks would be less efficiently adapted to absorption process for carbohydrates and protein than those from ES3 at the end of the hatch window.

  13. [Immunocytochemical studies on the phase of differentiation of hatching gland cells in brine shrimp, Artemia salina].

    PubMed

    Li, Ling; Fan, Ting Jun; Wang, Xiao Feng; Cong, Ri Shan; Yu, Qiu Tao; Zhong, Qi Wang

    2004-04-01

    Hatching enzyme (HE), synthesized in hatching gland cells (HGCs), plays vital roles in animal hatching. Immunocytochemical techniques employing anti-GST-UVS.2 antiserum, prepared from Xenopus HE and with specificity to brine shrimp HE, were first used to investigate the differentiation and variability of hatching gland cells (HGCs) in the hatching process of embryos of brine shrimp, Artemia salina, in this study. HGCs with immunoreactivity to anti-GST-UVS.2 antiserum were identified, for the first time, in brine shrimp embryos during hatching process. Immunocytochemical staining results showed that, (1) HE-positive immunoreactivity is really specific to Artemia HE, and its appearance and disappearance are closely correlated with the hatching process of Artemia salina. (2) Artemia HGCs, first appeared in embryos 5 hours before hatching and disappeared 4 hours after hatching, were also a transient type of cells, with an existence period of 9 hours. (3) The head portion of Artemia embryo is probably the initial position of HE secretion, and likely to be the main position of HE secretion as well. The detailed process and mechanism need to be studied. (4) The appearance of HGCs is in a synchronous mode from places all over the embryos, and their disappearance is also in a synchronous mode. (5) The number of HGCs increased gradually along with embryo development process and reached a maximum number at hatching. Contrarily, the number of HGCs decreased gradually after hatching, and HGCs disappeared 5 hours after hatching. However, the intensity of HE-positive reaction was almost at the same level at the period of HGCs'presence. (6) Artemia HGCs were distributed throughout the body of embryos at all time during their presence. Therefore, it can concluded that Artemia HGCs, as a transient type of cells, first appeared in embryos 4 hours before hatching and disappeared in embryos 5 hours after hatching, and with distinguished patterns of appearance, disappearance and distribution in embryos. What is the final destiny of Artemia HGCs after hatching? And what is the biological significance of remanet HGCs, still existing until 4 hours after hatching, in fresh-hatched Artemia larvae? Is it possible that the HGCs are involved in larvae yolk digestion? Moreover, what is the molecular mechanism of HGCs' synchronous sudden appearance and disappearance? All these questions remain to be further studied and approved.

  14. Using a Neural Network to Determine the Hatch Status of the AERI at the ARM North Slope of Alaska Site

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zwink, AB; Turner, DD

    2012-03-19

    The fore-optics of the Atmospheric Emitted Radiance Interferometer (AERI) are protected by an automated hatch to prevent precipitation from fouling the instrument's scene mirror (Knuteson et al. 2004). Limit switches connected with the hatch controller provide a signal of the hatch state: open, closed, undetermined (typically associated with the hatch being between fully open or fully closed during the instrument's sky view period), or an error condition. The instrument then records the state of the hatch with the radiance data so that samples taken when the hatch is not open can be removed from any subsequent analysis. However, the hatchmore » controller suffered a multi-year failure for the AERI located at the ARM North Slope of Alaska (NSA) Central Facility in Barrow, Alaska, from July 2006-February 2008. The failure resulted in misreporting the state of the hatch in the 'hatchOpen' field within the AERI data files. With this error there is no simple solution to translate what was reported back to the correct hatch status, thereby making it difficult for an analysis to determine when the AERI was actually viewing the sky. As only the data collected when the hatch is fully open are scientifically useful, an algorithm was developed to determine whether the hatch was open or closed based on spectral radiance data from the AERI. Determining if the hatch is open or closed in a scene with low clouds is non-trivial, as low opaque clouds may look very similar spectrally as the closed hatch. This algorithm used a backpropagation neural network; these types of neural networks have been used with increasing frequency in atmospheric science applications.« less

  15. 46 CFR 179.360 - Watertight integrity.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a cabin top; and (3) A hatch on a vessel that operates only on protected...

  16. 29 CFR 1918.42 - Hatch beam and pontoon bridles.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 7 2013-07-01 2013-07-01 false Hatch beam and pontoon bridles. 1918.42 Section 1918.42..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.42 Hatch beam and pontoon bridles. (a) Hatch beam and pontoon bridles shall be: (1) Long enough to...

  17. 29 CFR 1918.42 - Hatch beam and pontoon bridles.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 7 2012-07-01 2012-07-01 false Hatch beam and pontoon bridles. 1918.42 Section 1918.42..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.42 Hatch beam and pontoon bridles. (a) Hatch beam and pontoon bridles shall be: (1) Long enough to...

  18. 29 CFR 1918.42 - Hatch beam and pontoon bridles.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Hatch beam and pontoon bridles. 1918.42 Section 1918.42..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.42 Hatch beam and pontoon bridles. (a) Hatch beam and pontoon bridles shall be: (1) Long enough to...

  19. 46 CFR 179.360 - Watertight integrity.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a cabin top; and (3) A hatch on a vessel that operates only on protected...

  20. 46 CFR 179.360 - Watertight integrity.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a cabin top; and (3) A hatch on a vessel that operates only on protected...

  1. 46 CFR 179.360 - Watertight integrity.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a cabin top; and (3) A hatch on a vessel that operates only on protected...

  2. 29 CFR 1918.42 - Hatch beam and pontoon bridles.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 7 2011-07-01 2011-07-01 false Hatch beam and pontoon bridles. 1918.42 Section 1918.42..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.42 Hatch beam and pontoon bridles. (a) Hatch beam and pontoon bridles shall be: (1) Long enough to...

  3. 29 CFR 1918.42 - Hatch beam and pontoon bridles.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 7 2014-07-01 2014-07-01 false Hatch beam and pontoon bridles. 1918.42 Section 1918.42..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.42 Hatch beam and pontoon bridles. (a) Hatch beam and pontoon bridles shall be: (1) Long enough to...

  4. 46 CFR 179.360 - Watertight integrity.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a cabin top; and (3) A hatch on a vessel that operates only on protected...

  5. NSA AERI Hatch Correction Data Set

    DOE Data Explorer

    Turner, David

    2012-03-23

    From 2000-2008, the NSA AERI hatch was determined to be indicated as open too frequently. Analysis suggests that the hatch was actually opening and closing properly but that its status was not being correctly reported by the hatch controller to the datastream. An algorithm was written to determine the hatch status from the observed

  6. Extended hatching periods in the subantarctic lithodid crabs Lithodes santolla and Paralomis granulosa (Crustacea: Decapoda: Lithodidae)

    NASA Astrophysics Data System (ADS)

    Thatje, S.; Calcagno, J. A.; Lovrich, G. A.; Sartoris, F. J.; Anger, K.

    2003-06-01

    Temporal pattern of hatching was studied in the subantarctic lithodid crabs Lithodes santolla (Molina) and Paralomis granulosa (Jaquinot) from the Argentine Beagle Channel. In both species, larval hatching occurred in low daily numbers over an extended period of up to several weeks, depending on hatch size. Low daily hatching activity and low oxygen-consumption rates in freshly hatched P. granulosa larvae are discussed as life history adaptations to, and/or physiological constraints by, the environmental conditions of high latitudes.

  7. 9 CFR 82.9 - Interstate movement of hatching eggs from a quarantined area.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement of hatching eggs... Interstate movement of hatching eggs from a quarantined area. Hatching eggs from birds or poultry not known...) The hatching eggs are accompanied by a permit obtained in accordance with § 82.11; (b) Copies of the...

  8. 9 CFR 82.8 - Interstate movement of eggs, other than hatching eggs, from a quarantined area.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... than hatching eggs, from a quarantined area. 82.8 Section 82.8 Animals and Animal Products ANIMAL AND... Interstate movement of eggs, other than hatching eggs, from a quarantined area. (a) Eggs, other than hatching... establishment that processes the eggs, other than hatching eggs, for sale establishes procedures adequate to...

  9. 46 CFR 72.05-35 - Hatch covers and shifting boards.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 3 2014-10-01 2014-10-01 false Hatch covers and shifting boards. 72.05-35 Section 72.05... AND ARRANGEMENT Structural Fire Protection § 72.05-35 Hatch covers and shifting boards. (a) Wood hatch covers may be used between cargo spaces. Hatch covers in other locations shall meet the requirements for...

  10. 46 CFR 72.05-35 - Hatch covers and shifting boards.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 3 2013-10-01 2013-10-01 false Hatch covers and shifting boards. 72.05-35 Section 72.05... AND ARRANGEMENT Structural Fire Protection § 72.05-35 Hatch covers and shifting boards. (a) Wood hatch covers may be used between cargo spaces. Hatch covers in other locations shall meet the requirements for...

  11. 9 CFR 82.9 - Interstate movement of hatching eggs from a quarantined area.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Interstate movement of hatching eggs... Interstate movement of hatching eggs from a quarantined area. Hatching eggs from birds or poultry not known...) The hatching eggs are accompanied by a permit obtained in accordance with § 82.11; (b) Copies of the...

  12. 46 CFR 72.05-35 - Hatch covers and shifting boards.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 3 2011-10-01 2011-10-01 false Hatch covers and shifting boards. 72.05-35 Section 72.05... AND ARRANGEMENT Structural Fire Protection § 72.05-35 Hatch covers and shifting boards. (a) Wood hatch covers may be used between cargo spaces. Hatch covers in other locations shall meet the requirements for...

  13. 9 CFR 82.9 - Interstate movement of hatching eggs from a quarantined area.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Interstate movement of hatching eggs... Interstate movement of hatching eggs from a quarantined area. Hatching eggs from birds or poultry not known...) The hatching eggs are accompanied by a permit obtained in accordance with § 82.11; (b) Copies of the...

  14. Mechanism Guides Hatch Through Hatchway

    NASA Technical Reports Server (NTRS)

    Barron, Daniel R.; Kennedy, Steven E.

    1993-01-01

    Elliptical hatch designed to move through hatchway to make pressure-assisted seal with either side of bulkhead. Compact three-degree-of-freedom mechanism guides hatch through hatchway or holds hatch off to one side to facilitate passage of crew and/or equipment. Hatches and mechanisms used in submarines, pressure chambers (including hyperbaric treatment chambers), vacuum chambers, and vacuum-or-pressure test chambers.

  15. 46 CFR 72.05-35 - Hatch covers and shifting boards.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 3 2012-10-01 2012-10-01 false Hatch covers and shifting boards. 72.05-35 Section 72.05... AND ARRANGEMENT Structural Fire Protection § 72.05-35 Hatch covers and shifting boards. (a) Wood hatch covers may be used between cargo spaces. Hatch covers in other locations shall meet the requirements for...

  16. 9 CFR 82.9 - Interstate movement of hatching eggs from a quarantined area.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Interstate movement of hatching eggs... hatching eggs from a quarantined area. Hatching eggs from birds or poultry not known to be infected with or exposed to Newcastle disease may be moved interstate from a quarantined area only if: (a) The hatching...

  17. 9 CFR 82.9 - Interstate movement of hatching eggs from a quarantined area.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement of hatching eggs... Interstate movement of hatching eggs from a quarantined area. Hatching eggs from birds or poultry not known...) The hatching eggs are accompanied by a permit obtained in accordance with § 82.11; (b) Copies of the...

  18. Hatch plasticity in response to varied inundation frequency in Aedes albopictus.

    PubMed

    Vitek, Christopher J; Livdahl, Todd

    2009-07-01

    Eggs of container-breeding mosquitoes are able to withstand drought conditions as an egg and hatch when submerged. Frequent rainfall can be simulated by frequent submersion, and drought conditions can be simulated by infrequent submersion. We examined the hatch response of Aedes albopictus (Skuse) eggs to simulated drought conditions. Ae. albopictus eggs from a strain originating outside Kobe, Japan, were subjected to one of three treatments; high-frequency hatch stimulation consisting of submerging the eggs in a nutrient broth mixture every 3 d, low-frequency hatch stimulation consisting of submerging the eggs every 7 d, and delayed high-frequency hatch stimulation. Eggs that were subjected to lower-frequency stimulation showed a significant decrease in hatch delay, which was the opposite of the predicted response. This decrease in hatch delay may be an example of hatch plasticity in response to drought conditions. This response could not be explained as a result of the difference in the ages of the eggs on any given stimulus. A decreased hatch delay response to potential drought conditions combined with rapid larval development may enable Ae. albopictus, whose eggs are not as desiccation resistant as some other container-breeding mosquitoes, to survive extended drought.

  19. Effects of hatching time for larval ambystomatid salamanders

    USGS Publications Warehouse

    Boone, M.D.; Scott, D.E.; Niewiarowski, P.H.

    2002-01-01

    In aquatic communities, the phenology of breeding may influence species interactions. In the early-breeding marbled salamander, Ambystoma opacum, timing of pond filling may determine whether interactions among larvae are competitive or predatory. The objectives of our studies were to determine how time of egg hatching affected size, larval period, and survival to metamorphosis in A. opacum, and if early-hatching in A. opacum influenced the competitive and predator-prey relationships with smaller larvae of the mole salamander, Ambystoma talpoideum. Salamander larvae were reared from hatching through metamorphosis in large, outdoor enclosures located in a natural temporary pond in Aiken County, South Carolina, in two experiments. In study 1, we reared early- and late-hatching A. opacum larvae separately from hatching through metamorphosis. In study 2, we examined how early- versus late-hatching A. opacum affected a syntopic species, A. talpoideum. In general, early-hatching A. opacum were larger and older at metamorphosis, had greater survival, and left the pond earlier than late-hatching larvae. Ambystoma talpoideum reared in the presence of early-hatching A. opacum had lower survival than in controls, suggesting that A. opacum may predate upon A. talpoideum when they gain a growth advantage over later-hatching larvae. Our studies demonstrate that time of pond filling and phenology of breeding may influence population dynamics and alter the nature of relationships that develop among species.

  20. Unified hatch system

    NASA Technical Reports Server (NTRS)

    Hart, R. J.; Walkover, L. J.; Zosky, E. W.

    1971-01-01

    Special hatch sealing mechanism design increases safety, reliability, and convenience. Adaptations are possible for oceanographic and high-speed aircraft design, or for any system where quick-opening pressure hatch is required. In normal mode, hatching mechanism is manually operated from either side.

  1. Hatch cover

    NASA Technical Reports Server (NTRS)

    Allton, Charles S. (Inventor); Okane, James H. (Inventor)

    1989-01-01

    This invention relates to a hatch and more particularly to a hatch for a space vehicle where the hatch has a low volume sweep and can be easily manipulated from either side of the hatch. The hatch system includes an elliptical opening in a bulkhead and an elliptical hatch member. The hatch cover system includes an elliptical port opening in a housing and an elliptical cover member supported centrally by a rotational bearing for rotation about a rotational axis normal to the cover member and by pivot pins in a gimbal member for pivotal movement about axes perpendicular to the rotational axis. Arm members support the gimbal member pivotally by pivot members so that upon rotation and manipulation the cover member can be articulatedly moved from a closed position to the port opening to an out of the way position with a minimum of volume sweep by the cover member.

  2. The effect of overwing hatch placement on evacuation from smaller transport aircraft.

    PubMed

    Wilson, Rebecca L; Muir, Helen C

    2009-08-01

    Overwing exits are installed on a number of smaller transport aircraft. With a traditional overwing exit, once released, the hatch is not attached to the fuselage and will fall into the cabin. To operate, the hatch has to be brought inwards, manoeuvred and placed in a location where it does not obstruct egress. Accidents and experimental studies have shown that the hatch is not always disposed of into an appropriate location. Evacuation trials from a smaller transport aircraft cabin were conducted. The placement of the exit hatch was manipulated. The results indicated that hatch placement had a significant effect on passenger evacuation rates from a smaller transport aircraft, with the internal placement tested resulting in slower evacuation rates. The study has highlighted the importance of operators disposing of the hatch into a location whereby it does not impede egress. One way to ensure this would be the installation of an automatically disposed hatch.

  3. Hatching behavior in turtles.

    PubMed

    Spencer, Ricky-John; Janzen, Fredric J

    2011-07-01

    Incubation temperature plays a prominent role in shaping the phenotypes and fitness of embryos, including affecting developmental rates. In many taxa, including turtles, eggs are deposited in layers such that thermal gradients alter developmental rates within a nest. Despite this thermal effect, a nascent body of experimental work on environmentally cued hatching in turtles has revealed unexpected synchronicity in hatching behavior. This review discusses environmental cues for hatching, physiological mechanisms behind synchronous hatching, proximate and ultimate causes for this behavior, and future directions for research. Four freshwater turtle species have been investigated experimentally, with hatching in each species elicited by different environmental cues and responding via various physiological mechanisms. Hatching of groups of eggs in turtles apparently involves some level of embryo-embryo communication and thus is not a purely passive activity. Although turtles are not icons of complex social behavior, life-history theory predicts that the group environment of the nest can drive the evolution of environmentally cued hatching.

  4. 1,10-Phenanthroline and its derivatives are novel hatching stimulants for soybean cyst nematodes.

    PubMed

    Nonaka, Shiori; Katsuyama, Tsutomu; Kondo, Tatsuhiko; Sasaki, Yasuyuki; Asami, Tadao; Yajima, Shunsuke; Ito, Shinsaku

    2016-11-01

    Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is a plant-parasitic nematode and one of the most serious soybean pests. Herein, we present the heterocyclic compound 1,10-phenanthroline (Phen) and its derivatives as novel hatching stimulants for SCN. Phen treatment promoted hatching of second-stage juveniles of SCNs in a concentration-dependent manner. In addition, the hatching of SCNs following treatment with Phen occurred more rapidly than that following treatment with the known hatching stimulant, glycinoeclepin A (GEA). Furthermore, the co-application of Phen and GEA enhanced SCN hatching rate compared with that of Phen or GEA alone. A structure-activity relationship study for Phen derivatives suggested that 2,2'-bipyridine is the essential structure of the SCN-hatching stimulants. These results suggest that Phen and its derivatives activate different hatching pathways of SCNs from GEA. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. 46 CFR 171.124 - Watertight integrity above the margin line in a vessel less than 100 gross tons.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 100 gross tons. (a) Each hatch exposed to the weather must be watertight; except that, the following hatches may be weathertight: (1) Each hatch on a watertight trunk that extends at least 12 inches (30.5 centimeters) above the weather deck. (2) Each hatch in a cabin top. (3) Each hatch on a vessel that operates...

  6. 46 CFR 171.124 - Watertight integrity above the margin line in a vessel less than 100 gross tons.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 100 gross tons. (a) Each hatch exposed to the weather must be watertight; except that, the following hatches may be weathertight: (1) Each hatch on a watertight trunk that extends at least 12 inches (30.5 centimeters) above the weather deck. (2) Each hatch in a cabin top. (3) Each hatch on a vessel that operates...

  7. 46 CFR 171.124 - Watertight integrity above the margin line in a vessel less than 100 gross tons.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 100 gross tons. (a) Each hatch exposed to the weather must be watertight; except that, the following hatches may be weathertight: (1) Each hatch on a watertight trunk that extends at least 12 inches (30.5 centimeters) above the weather deck. (2) Each hatch in a cabin top. (3) Each hatch on a vessel that operates...

  8. 46 CFR 171.124 - Watertight integrity above the margin line in a vessel less than 100 gross tons.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 100 gross tons. (a) Each hatch exposed to the weather must be watertight; except that, the following hatches may be weathertight: (1) Each hatch on a watertight trunk that extends at least 12 inches (30.5 centimeters) above the weather deck. (2) Each hatch in a cabin top. (3) Each hatch on a vessel that operates...

  9. 46 CFR 171.124 - Watertight integrity above the margin line in a vessel less than 100 gross tons.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 100 gross tons. (a) Each hatch exposed to the weather must be watertight; except that, the following hatches may be weathertight: (1) Each hatch on a watertight trunk that extends at least 12 inches (30.5 centimeters) above the weather deck. (2) Each hatch in a cabin top. (3) Each hatch on a vessel that operates...

  10. Host Status of Different Potato (Solanum tuberosum) Varieties and Hatching in Root Diffusates of Globodera ellingtonae

    PubMed Central

    Zasada, Inga A.; Peetz, Amy; Wade, Nadine; Navarre, Roy A.; Ingham, Russ E.

    2013-01-01

    Globodera ellingtonae was detected in Oregon in 2008. In order to make decisions regarding the regulation of this nematode, knowledge of its biology is required. We determined the host status of a diversity of potato (Solanum tuberosum) varieties in soil-based experiments and identified hatching stimulants in in vitro hatching assays. ‘Russet Burbank,’ ‘Desiree,’ ‘Modac,’ ‘Norland,’ ‘Umatilla,’ and ‘Yukon Gold’ were good hosts (RF > 14) for G. ellingtonae. Potato varieties ‘Maris Piper,’ ‘Atlantic,’ and ‘Satina,’ all which contain the Ro1 gene that confers resistance to G. rostochiensis, were not hosts for G. ellingtonae. In in vitro hatching assays, G. ellingtonae hatched readily in the presence of diffusates from potato (PRD) and tomato (Solanum lycopersicum; TRD). Egg hatch occurred in an average of between 87% and 90% of exposed cysts, with an average of between 144 and 164 juveniles emerging per cyst, from PRD- and TRD-treated cysts, respectively. This nematode hatched rapidly in the presence of PRD and TRD, with at least 66% of total hatch occurring by day 3 of exposure. There was no dose-response of egg hatch to concentrations of PRD or TRD ranging from 1:5 to 1:100 diffusate to water. When G. ellingtonae was exposed to root diffusates from 21 different plants, hatch occurred in 0% to 70% of exposed cysts, with an average of between 0 to 27 juveniles emerging per cyst. When root diffusate-exposed cysts were subsequently transferred to PRD to test viability, root diffusates from arugula (Eruca sativa), sudangrass (Sorghum bicolor subsp. drummondii), and common vetch (Vicia sativa) continued to inhibit egg hatch compared with the other root diffusates or water in which hatch occurred readily (60 to 182 juveniles emerging per cyst). Previously known hatching stimulants of G. rostochiensis and G. pallida, sodium metavanadate, sodium orthovanadate, and sodium thiocyanate, stimulated some egg hatch. Although, Globodera ellingtonae hatched readily in PRD and TRD and reproduced on potato, the pathogenicity of this nematode on potato remains to be determined. PMID:24115784

  11. Host Status of Different Potato (Solanum tuberosum) Varieties and Hatching in Root Diffusates of Globodera ellingtonae.

    PubMed

    Zasada, Inga A; Peetz, Amy; Wade, Nadine; Navarre, Roy A; Ingham, Russ E

    2013-09-01

    Globodera ellingtonae was detected in Oregon in 2008. In order to make decisions regarding the regulation of this nematode, knowledge of its biology is required. We determined the host status of a diversity of potato (Solanum tuberosum) varieties in soil-based experiments and identified hatching stimulants in in vitro hatching assays. 'Russet Burbank,' 'Desiree,' 'Modac,' 'Norland,' 'Umatilla,' and 'Yukon Gold' were good hosts (RF > 14) for G. ellingtonae. Potato varieties 'Maris Piper,' 'Atlantic,' and 'Satina,' all which contain the Ro1 gene that confers resistance to G. rostochiensis, were not hosts for G. ellingtonae. In in vitro hatching assays, G. ellingtonae hatched readily in the presence of diffusates from potato (PRD) and tomato (Solanum lycopersicum; TRD). Egg hatch occurred in an average of between 87% and 90% of exposed cysts, with an average of between 144 and 164 juveniles emerging per cyst, from PRD- and TRD-treated cysts, respectively. This nematode hatched rapidly in the presence of PRD and TRD, with at least 66% of total hatch occurring by day 3 of exposure. There was no dose-response of egg hatch to concentrations of PRD or TRD ranging from 1:5 to 1:100 diffusate to water. When G. ellingtonae was exposed to root diffusates from 21 different plants, hatch occurred in 0% to 70% of exposed cysts, with an average of between 0 to 27 juveniles emerging per cyst. When root diffusate-exposed cysts were subsequently transferred to PRD to test viability, root diffusates from arugula (Eruca sativa), sudangrass (Sorghum bicolor subsp. drummondii), and common vetch (Vicia sativa) continued to inhibit egg hatch compared with the other root diffusates or water in which hatch occurred readily (60 to 182 juveniles emerging per cyst). Previously known hatching stimulants of G. rostochiensis and G. pallida, sodium metavanadate, sodium orthovanadate, and sodium thiocyanate, stimulated some egg hatch. Although, Globodera ellingtonae hatched readily in PRD and TRD and reproduced on potato, the pathogenicity of this nematode on potato remains to be determined.

  12. Significance of chick quality score in broiler production.

    PubMed

    van de Ven, L J F; van Wagenberg, A V; Uitdehaag, K A; Groot Koerkamp, P W G; Kemp, B; van den Brand, H

    2012-10-01

    The quality of day old chicks is crucial for profitable broiler production, but a difficult trait to define. In research, both qualitative and quantitative measures are used with variable predictive value for subsequent performance. In hatchery practice, chick quality is judged on a binomial scale, as chicks are divided into first grade (Q1-saleable) and second grade (Q2) chicks right after hatch. Incidences and reasons for classifying chicks as Q2, and potential of these chicks for survival and post-hatch performance have hardly been investigated, but may provide information for flock performance. We conducted an experiment to investigate (1) the quality of a broiler flock and the relation with post-hatch flock performance based on a qualitative score (Pasgar©score) of Q1 chicks and based on the incidence of Q2 chicks and (2) the reasons for classifying chicks as Q2, and the potential of these chicks for survival and post-hatch growth. The performance was followed of Q1 and Q2 chicks obtained from two breeder flocks that hatched in two different hatching systems (a traditional hatcher or a combined hatching and brooding system, named Patio). Eggs were incubated until embryo day 18, when they were transferred to one of the two hatching systems. At embryo day 21/post-hatch day 0, all chicks from the hatcher (including Q2 chicks) were brought to Patio, where the hatchery manager marked the Q2 chicks from both flocks and hatching systems and registered apparent reasons for classifying these chicks as Q2. Chick quality was assessed of 100 Q1 chicks from each flock and hatching system. Weights of all chicks were determined at days 0, 7, 21 and 42. There were no correlations between mean Pasgar©score and post-hatch growth or mortality, and suboptimal navel quality was the only quality trait associated with lower post-hatch growth. Growth was clearly affected by breeder flock and hatching system, which could not be linked to mean Pasgar©score or incidence of Q2 chicks. Q2 chicks showed lower post-hatch growth compared to Q1 chicks but effects on flock performance at slaughter weight were limited because early mortality in Q2 chicks was high (62.50% at 7 days). We concluded that chick qualitative scores and the incidence of Q2 chicks may be informative for the quality of incubation, but are not predictive for post-hatch flock performance. Culling Q2 chicks after hatch is well-founded in terms of both animal welfare and profitability.

  13. 7 CFR 60.111 - Hatched.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Hatched. 60.111 Section 60.111 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.111 Hatched. Hatched means emerged from the egg. ...

  14. 7 CFR 60.111 - Hatched.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Hatched. 60.111 Section 60.111 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.111 Hatched. Hatched means emerged from the egg. ...

  15. 7 CFR 60.111 - Hatched.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Hatched. 60.111 Section 60.111 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.111 Hatched. Hatched means emerged from the egg. ...

  16. 7 CFR 60.111 - Hatched.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Hatched. 60.111 Section 60.111 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections... AND SHELLFISH General Provisions Definitions § 60.111 Hatched. Hatched means emerged from the egg. ...

  17. Pressure Seal For Frequently Opened Hatch

    NASA Technical Reports Server (NTRS)

    Kennedy, Steven E.; Kramer, Joel M.

    1994-01-01

    Pressure-assisted seal for frequently opened hatch includes two sealing rings retained positively so not pulled out during opening. Seal makes contact with hatch well before hatch starts to squeeze rings extending distance over which seal becomes engaged. Improvements include more-secure mounting, redundancy, and better initial sealing action. Also minimizes loss of gas during closure by deflecting inward and closing gap. This action helps differential pressure to force hatch closed.

  18. The effect of overwing hatch placement on evacuation from smaller transport aircraft.

    PubMed

    Wilson, Rebecca L; Muir, Helen C

    2010-02-01

    Overwing exits are installed on a number of smaller transport aircraft. With a traditional overwing exit, once released, the hatch is not attached to the fuselage and will fall into the cabin. To operate, the hatch has to be brought inwards, manoeuvred and placed in a location where it does not obstruct egress. Accidents and experimental studies have shown that the hatch is not always disposed of into an appropriate location. Evacuation trials from a smaller transport aircraft cabin were conducted. The placement of the exit hatch was manipulated. The results indicated that hatch placement had a significant effect on passenger evacuation rates from a smaller transport aircraft, with the internal placement tested resulting in slower evacuation rates. The study has highlighted the importance of operators disposing of the hatch into a location whereby it does not impede egress. One way to ensure this would be the installation of an automatically disposed hatch. Statement of Relevance: It is important that all occupants can evacuate an aircraft rapidly if required. The influence of overwing hatch placement on evacuation from smaller transport aircraft was addressed Evacuation trials concluded that an inappropriately placed hatch can negatively influence evacuation rates. Improvements to exit design and passenger education were suggested.

  19. Behavior of a nuclear steel containment equipment hatch at large strain

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fanous, F.; Greimann, L.

    1988-05-01

    During a severe accident, buckling of a steel containment hatch door, large deformation and ovaling of the hatch sleeve are potential causes of mismatch at the sealing surface which can result in a leakage path. A three-dimensional nonlinear finite element analysis of a typical steel containment/sleeve/hatch assembly that includes containment stiffeners, pretensioned swing bolts, and hatch door geometric imperfection is presented. The analysis was carried out to the nonlinear range up to large strains. The results indicated that the buckling load occurs at pressure, far above that which causes gross yielding of the shell plate. Although buckling of the hatchmore » door increased the relative motions of the hatch sleeve and the hatch door, the motions remained sufficiently small to prevent leakage.« less

  20. Inheritance and World Variation in Thermal Requirements for Egg Hatch in Lymantria dispar (Lepidoptera: Erebidae).

    PubMed

    Keena, M A

    2016-02-01

    Mode of inheritance of hatch traits in Lymantria dispar L. was determined by crossing populations nearly fixed for the phenotypic extremes. The nondiapausing phenotype was inherited via a single recessive gene and the phenotype with reduced low temperature exposure requirements before hatch was inherited via a single dominant gene. There was no evidence for sex-linkage or cytoplasmic effects with either gene. Eggs from 43 geographic populations were evaluated for hatch characteristics after being held for 60 d at 5°C followed by incubation at 25°C. There was considerable variation both within and among the populations in the proportion able to hatch, time to first hatch, and average time to hatch. Egg masses with reduced requirement for low temperatures before the eggs were ready to hatch were present in all subspecies of L. dispar and the phenotype was not fixed in most populations. The populations clustered into three distinct groups, and climatic variables were found to be rough predictors of those groups. Variation in hatch phenotypes between populations is likely an adaptation to local climate and within a population provides a bet-hedging strategy to ensure that at least some hatch synchronizes with host leaf-out. Continued vigilance to prevent movement of populations both within and between countries is warranted, because some of the alleles that confer nondiapause or reduced low temperature requirements before egg hatch are not present in all populations and their introduction would increase variation in egg hatch within a population. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by a US Government employee and is in the public domain in the US.

  1. Effects of Hatching Time on Behavior and Weight Development of Chickens

    PubMed Central

    Løtvedt, Pia; Jensen, Per

    2014-01-01

    The length of the embryonic period varies both among and within species and can affect the individual phenotype in many ways, both physiologically and behaviorally. In chickens, the hatch window may last 24–48 hours (up to 10% of the incubation time), and studies have shown that incubation length may affect post-hatch growth and physiology. However, little is known about effects on behavior. We therefore investigated how behavior variation correlates with hatching time in the early life of chickens. We also measured egg weight and egg weight loss in relation to hatching time, as well as post-hatch growth. For females, there was a negative correlation between hatch time and body weight from day 4 and throughout the experiment. For males, such a correlation was only observed when testing all hatched males up until day 10. The birds were exposed to a number of behavioral tests, and a principal components analysis was performed on the variables, resulting in four components. For the largest component, termed “Passivity”, a tendency of a difference was found between early and middle male hatchers. Furthermore, a significant difference between early and middle male hatchers was found in the second component, termed “Response to novelty”. In a spatial learning test, late hatchers tended to learn slower. The behavior of females was not significantly affected by hatching time in any of these tests. This study is among the first to demonstrate a link between time of hatching and early behavior in a precocial species like the chicken, and may help shedding light on the evolutionary trade-offs between incubation length and post-hatch traits. The results may also be relevant from a perspective of stress coping and therefore also for animal welfare and productivity in the chicken industry. The mechanisms linking hatching time with post-hatch phenotype remain to be investigated. PMID:25058654

  2. Egg Hatch Rate and Nymphal Survival of the Bed Bug (Hemiptera: Cimicidae) After Exposure to Insecticide Sprays.

    PubMed

    Hinson, K R; Benson, E P; Zungoli, P A; Bridges, W C; Ellis, B R

    2016-12-01

    Few studies have addressed the efficacy of insecticides used against eggs and first-instar nymphs of the bed bug, Cimex lectularius L. (Hemiptera: Cimicidae). Insect eggs are often resistant to insecticides; therefore, information on which products are effective is important. We evaluated the efficacy of four commonly used insecticide sprays applied directly to bed bug eggs. We also evaluated the efficacy of these insecticides to first-instar nymphs exposed to residuals resulting from directly spraying eggs. Temprid SC (beta-cyfluthrin, imidacloprid) was the most effective insecticide at preventing egg hatch (13% hatch rate) for pyrethroid-resistant, field-strain (Jersey City) bed bugs compared with a control (water [99% hatch rate]), Bedlam (MGK-264, sumithrin [84% hatch rate]), Demand CS (lambda-cyhalothrin [91% hatch rate]), and Phantom SC (chlorfenapyr [95% hatch rate]). Demand CS and Temprid SC were most effective at preventing egg hatch (0%) for an insecticide-susceptible (Harold Harlan) strain, followed by Bedlam (28%). Phantom SC produced a hatch rate similar to the control (97% and 96%, respectively). Harold Harlan-strain nymphs showed 100% survival for the control but 0% survival for Bedlam and Phantom SC. Jersey City-strain nymphs showed 100% survival for the control, 99% survival for Bedlam, 0% survival for Demand CS, 4% survival for Phantom SC, and 38% survival for Temprid SC. Demand CS was less effective at preventing hatch (91% hatch rate) of Jersey City-strain nymphs but was the only product to kill all nymphs (0% survival). One of the least effective products for preventing Jersey City-strain egg hatch (Phantom SC, 95% hatch rate) was the second most effective at killing nymphs, leaving only six of 141 alive. These findings indicate that survival of directly sprayed eggs and residually exposed, first-instar nymphs varies by strain, life stage, and product used. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  3. Biological monitoring of welders' exposure to chromium, molybdenum, tungsten and vanadium.

    PubMed

    Ellingsen, Dag G; Chashchin, Maxim; Berlinger, Balazs; Fedorov, Vladimir; Chashchin, Valery; Thomassen, Yngvar

    2017-05-01

    Welders are exposed to a number of metallic elements during work. Bioaccessability, that is important for element uptake, has been little studied. This study addresses bioaccessability and uptake of chromium (Cr), molybdenum (Mo), tungsten (W) and vanadium (V) among welders. Bioaccessability of Cr, Mo, V and W was studied in airborne particulate matter collected by personal sampling of the workroom air among shipyard welders by using the lung lining fluid simulant Hatch solution. Associations between concentrations of Hatch soluble and non-soluble elements (Hatch sol and Hatch non-sol ) and concentrations of the four elements in whole blood, serum, blood cells and urine were studied. Air concentrations of the four elements were low. Only a small fraction of Cr, V and W was Hatch sol , while similar amounts of Mo were Hatch sol and Hatch non-sol . Welders (N=70) had statistically significantly higher concentrations of all four elements in urine and serum when compared to referents (N=74). Highly statistically significant associations were observed between urinary W and Hatch sol W (p<0.001) and serum V and Hatch sol V (p<0.001), in particular when air samples collected the day before collection of biological samples were considered. Associations between Hatch sol elements in air and their biological concentrations were higher than when Hatch non-sol concentrations were considered. Associations were generally higher when air samples collected the day before biological sampling were considered as compared to air samples collected two days before. Copyright © 2017 Elsevier GmbH. All rights reserved.

  4. Effects of reservoir hydrology on reproduction by largemouth bass and spotted bass in Normandy Reservoir, Tennessee

    USGS Publications Warehouse

    Sammons, S.M.; Dorsey, L.G.; Bettoli, P.W.; Fiss, F.C.

    1999-01-01

    Age-O largemouth bass Micropterus salmoides and spotted bass M. punctulatus were collected from Normandy Reservoir, Tennessee, 1992-1996, to evaluate effects of reservoir hydrology and hatching of shad Dorosoma spp. on hatching and first-year growth and survival of these two species. Fish were collected in cove rotenone samples in early August and electrofishing samples biweekly throughout the summer; hatch dates and age-specific growth for both species were determined from cove samples with sagittal otoliths. Hatching of both species ranged from early April to early June. Initiation of largemouth bass spawning, but not spotted bass spawning, was positively related to the first day water levels achieved full pool. Mean hatch dates of both species were positively related to the first day of full pool. Timing of spawning for both species was not related to water temperature, Largemouth bass exhibited bimodal length-frequency distributions by midsummer in two wet years and length frequencies were unimodal in dry years; spotted bass always formed unimodal length-frequency distributions. When largemouth bass exhibited bimodal length distributions, earlier hatched fish grew faster than later hatched fish. Spotted bass grew at similar rates, regardless of hatch date, every year except during 1992 when later hatched fish grew faster than earlier hatched fish. Weekly survival of largemouth bass in their first summer was positively related to reservoir water level. First-year growth of both species was not directly affected by the timing of threadfin shad D. petenense or gizzard shad D. cepedianum hatching.

  5. 9 CFR 147.22 - Hatching egg sanitation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Hatching egg sanitation. 147.22... Procedures § 147.22 Hatching egg sanitation. Hatching eggs should be collected from the nests at frequent... practices should be observed: (a) Cleaned and disinfected containers, such as egg flats, should be used in...

  6. 45 CFR 1226.10 - Hatch Act restrictions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Hatch Act restrictions. 1226.10 Section 1226.10... SERVICE PROHIBITIONS ON ELECTORAL AND LOBBYING ACTIVITIES Volunteer Activities § 1226.10 Hatch Act... Hatch Act, subchapter III, of chapter 73, title 5, United States Code. Full time volunteers shall not...

  7. 46 CFR 122.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Watertight doors and watertight hatches. 122.610 Section... Markings Required § 122.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25 millimeters (1 inch) high...

  8. 46 CFR 116.1160 - Watertight integrity.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... and Watertight Integrity of Weather Decks § 116.1160 Watertight integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a...

  9. Section BB Hatch Coating; Framing Plan on Line C Lodging ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Section B-B Hatch Coating; Framing Plan on Line C Lodging Knees at Hatch; Elevation A-A Hull Framing; Section at Hatch Frame 36, Starboard Looking Aft; Midship Section Frame 37, Port Looking Aft - Steam Schooner WAPAMA, Kaiser Shipyard No. 3 (Shoal Point), Richmond, Contra Costa County, CA

  10. 46 CFR 122.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Watertight doors and watertight hatches. 122.610 Section... Markings Required § 122.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25 millimeters (1 inch) high...

  11. 29 CFR 1918.31 - Hatch coverings.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 7 2011-07-01 2011-07-01 false Hatch coverings. 1918.31 Section 1918.31 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.31 Hatch coverings. (a) No cargo... partially opened intermediate deck unless either the hatch at that deck is sufficiently covered or an...

  12. 46 CFR 169.747 - Watertight doors and hatches.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Watertight doors and hatches. 169.747 Section 169.747... Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.747 Watertight doors and hatches. Each watertight door and watertight hatch must be marked on both sides in at least 1-inch letters...

  13. 46 CFR 169.827 - Hatches and other openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Hatches and other openings. 169.827 Section 169.827... Operations Tests, Drills, and Inspections § 169.827 Hatches and other openings. The master is responsible for seeing that all hatches, openings in the hull, and watertight doors are properly closed tight. ...

  14. 46 CFR 122.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Watertight doors and watertight hatches. 122.610 Section... Markings Required § 122.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25 millimeters (1 inch) high...

  15. 29 CFR 1918.31 - Hatch coverings.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Hatch coverings. 1918.31 Section 1918.31 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.31 Hatch coverings. (a) No cargo... partially opened intermediate deck unless either the hatch at that deck is sufficiently covered or an...

  16. 46 CFR 185.330 - Hatches and other openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Hatches and other openings. 185.330 Section 185.330... TONS) OPERATIONS Miscellaneous Operating Requirements § 185.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or rivers routes in calm weather, all hatches and openings...

  17. 46 CFR 169.827 - Hatches and other openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Hatches and other openings. 169.827 Section 169.827... Operations Tests, Drills, and Inspections § 169.827 Hatches and other openings. The master is responsible for seeing that all hatches, openings in the hull, and watertight doors are properly closed tight. ...

  18. 46 CFR 169.747 - Watertight doors and hatches.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Watertight doors and hatches. 169.747 Section 169.747... Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.747 Watertight doors and hatches. Each watertight door and watertight hatch must be marked on both sides in at least 1-inch letters...

  19. 46 CFR 122.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Watertight doors and watertight hatches. 122.610 Section... Markings Required § 122.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25 millimeters (1 inch) high...

  20. 46 CFR 169.827 - Hatches and other openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Hatches and other openings. 169.827 Section 169.827... Operations Tests, Drills, and Inspections § 169.827 Hatches and other openings. The master is responsible for seeing that all hatches, openings in the hull, and watertight doors are properly closed tight. ...

  1. 46 CFR 122.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

  2. 46 CFR 122.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

  3. 46 CFR 185.330 - Hatches and other openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Hatches and other openings. 185.330 Section 185.330... TONS) OPERATIONS Miscellaneous Operating Requirements § 185.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or rivers routes in calm weather, all hatches and openings...

  4. 45 CFR 1226.10 - Hatch Act restrictions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Hatch Act restrictions. 1226.10 Section 1226.10... SERVICE PROHIBITIONS ON ELECTORAL AND LOBBYING ACTIVITIES Volunteer Activities § 1226.10 Hatch Act... Hatch Act, subchapter III, of chapter 73, title 5, United States Code. Full time volunteers shall not...

  5. 46 CFR 122.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

  6. 46 CFR 169.827 - Hatches and other openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Hatches and other openings. 169.827 Section 169.827... Operations Tests, Drills, and Inspections § 169.827 Hatches and other openings. The master is responsible for seeing that all hatches, openings in the hull, and watertight doors are properly closed tight. ...

  7. 45 CFR 1226.10 - Hatch Act restrictions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Hatch Act restrictions. 1226.10 Section 1226.10... SERVICE PROHIBITIONS ON ELECTORAL AND LOBBYING ACTIVITIES Volunteer Activities § 1226.10 Hatch Act... Hatch Act, subchapter III, of chapter 73, title 5, United States Code. Full time volunteers shall not...

  8. 46 CFR 185.330 - Hatches and other openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Hatches and other openings. 185.330 Section 185.330... TONS) OPERATIONS Miscellaneous Operating Requirements § 185.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or rivers routes in calm weather, all hatches and openings...

  9. 46 CFR 169.747 - Watertight doors and hatches.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Watertight doors and hatches. 169.747 Section 169.747... Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.747 Watertight doors and hatches. Each watertight door and watertight hatch must be marked on both sides in at least 1-inch letters...

  10. 46 CFR 185.330 - Hatches and other openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Hatches and other openings. 185.330 Section 185.330... TONS) OPERATIONS Miscellaneous Operating Requirements § 185.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or rivers routes in calm weather, all hatches and openings...

  11. 46 CFR 185.330 - Hatches and other openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Hatches and other openings. 185.330 Section 185.330... TONS) OPERATIONS Miscellaneous Operating Requirements § 185.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or rivers routes in calm weather, all hatches and openings...

  12. 46 CFR 169.827 - Hatches and other openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Hatches and other openings. 169.827 Section 169.827... Operations Tests, Drills, and Inspections § 169.827 Hatches and other openings. The master is responsible for seeing that all hatches, openings in the hull, and watertight doors are properly closed tight. ...

  13. 29 CFR 1918.31 - Hatch coverings.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 7 2013-07-01 2013-07-01 false Hatch coverings. 1918.31 Section 1918.31 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.31 Hatch coverings. (a) No cargo... partially opened intermediate deck unless either the hatch at that deck is sufficiently covered or an...

  14. 29 CFR 1918.31 - Hatch coverings.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 7 2014-07-01 2014-07-01 false Hatch coverings. 1918.31 Section 1918.31 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.31 Hatch coverings. (a) No cargo... partially opened intermediate deck unless either the hatch at that deck is sufficiently covered or an...

  15. 46 CFR 169.747 - Watertight doors and hatches.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Watertight doors and hatches. 169.747 Section 169.747... Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.747 Watertight doors and hatches. Each watertight door and watertight hatch must be marked on both sides in at least 1-inch letters...

  16. 46 CFR 122.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Watertight doors and watertight hatches. 122.610 Section... Markings Required § 122.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25 millimeters (1 inch) high...

  17. 46 CFR 169.747 - Watertight doors and hatches.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Watertight doors and hatches. 169.747 Section 169.747... Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.747 Watertight doors and hatches. Each watertight door and watertight hatch must be marked on both sides in at least 1-inch letters...

  18. 46 CFR 122.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

  19. 46 CFR 122.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

  20. 29 CFR 1918.31 - Hatch coverings.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 7 2012-07-01 2012-07-01 false Hatch coverings. 1918.31 Section 1918.31 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.31 Hatch coverings. (a) No cargo... partially opened intermediate deck unless either the hatch at that deck is sufficiently covered or an...

  1. 46 CFR 116.1160 - Watertight integrity.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and Watertight Integrity of Weather Decks § 116.1160 Watertight integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a...

  2. 46 CFR 116.1160 - Watertight integrity.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... and Watertight Integrity of Weather Decks § 116.1160 Watertight integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a...

  3. 46 CFR 116.1160 - Watertight integrity.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... and Watertight Integrity of Weather Decks § 116.1160 Watertight integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a...

  4. 46 CFR 116.1160 - Watertight integrity.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and Watertight Integrity of Weather Decks § 116.1160 Watertight integrity. (a) A hatch exposed to the weather must be watertight, except that the following hatches may be weathertight: (1) A hatch on a watertight trunk that extends at least 305 millimeters (12 inches) above the weather deck; (2) A hatch in a...

  5. STS-38 Pilot Culbertson rolls through CCT side hatch during egress training

    NASA Technical Reports Server (NTRS)

    1990-01-01

    STS-38 Pilot Frank L. Culbertson, wearing launch and entry suit (LES) and launch and entry helmet (LEH), rolls through the side hatch of the crew compartment trainer (CCT) located in JSC's Mockup and Integration Laboratory (MAIL) Bldg 9A. Assisted by technicians, Culbertson practices emergency egress through the side hatch using the crew escape system (CES) pole which extends out the side hatch. The inflated safety cushion breaks Culbertson's fall as he rolls out of the side hatch.

  6. STS-38 Pilot Culbertson rolls through CCT side hatch during egress training

    NASA Image and Video Library

    1990-03-05

    STS-38 Pilot Frank L. Culbertson, wearing launch and entry suit (LES) and launch and entry helmet (LEH), rolls through the side hatch of the crew compartment trainer (CCT) located in JSC's Mockup and Integration Laboratory (MAIL) Bldg 9A. Assisted by technicians, Culbertson practices emergency egress through the side hatch using the crew escape system (CES) pole which extends out the side hatch. The inflated safety cushion breaks Culbertson's fall as he rolls out of the side hatch.

  7. The 1984 ARI Survey of Army Recruits: Tabular Description of NPS Army Reserve Accessions. Volume 2

    DTIC Science & Technology

    1986-05-01

    12 PROB. 0.1867 I 186 T261 — NATCH TV PROG’NBA BASKETBALL NARK ONE LETTER FOR EACH OF THE FOLLOWING PROGRAMS OR PROGRAMMING TYPES ON TV: NBA ...Major league baseball — regular seaaon games 105. Major league baaaball playoffs 106. World Series 107. NBA baaketball 106. College basketball 109...BASEBALL PLAYOFFS HATCH TV PROG:WORLD SERIES HATCH TV PROG:NBA BASKETBALL HATCH TV PROG:COLLEGE BASKETBALL HATCH TV PROG:NHL HOCKEY HATCH TV

  8. The behaviour of cow blastocyst in vitro: cinematographic and morphometric analysis.

    PubMed Central

    Massip, A; Mulnard, J; Vanderzwalmen, P; Hanzen, C; Ectors, F

    1982-01-01

    The behaviour of the cow blastocyst in vitro was studied by time-lapse cinematography and analysed by morphometry. Three types of behaviour were observed: continuous expansion followed by hatching; discontinuous expansion interrupted by few contractions and followed by hatching; discontinuous expansion interrupted by several rapid contractions without hatching. This demonstrated that the pulsatile activity of the blastocyst is not a necessary condition of hatching but also that only a moderate pulsatile activity is compatible with normal hatching. The time of hatching in vitro corresponded approximately with the time of zona loss in vivo (9-10 days). Rupture of the zona occurred at any point of the trophoblast layer. Hatching by herniation through a reduced opening of the zona was occasionally observed. The behavior of the embryos from a particular animal was very similar but differences were noted between embryos from different animals. Images Fig. 3 PMID:7076563

  9. 29 CFR 1918.41 - Coaming clearances.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.41 Coaming... five feet (1.52 m) high is stowed within three feet (.91 m) of the hatch coaming and employees handling hatch beams and hatch covers are not protected by a coaming at least 24-inch (.61 m) high, a taut...

  10. 29 CFR 1918.41 - Coaming clearances.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.41 Coaming... five feet (1.52 m) high is stowed within three feet (.91 m) of the hatch coaming and employees handling hatch beams and hatch covers are not protected by a coaming at least 24-inch (.61 m) high, a taut...

  11. 46 CFR 185.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Watertight doors and watertight hatches. 185.610 Section... (UNDER 100 GROSS TONS) OPERATIONS Markings Required § 185.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25...

  12. 46 CFR 131.520 - Hatches and other openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Hatches and other openings. 131.520 Section 131.520..., Drills, and Inspections § 131.520 Hatches and other openings. Before any vessel leaves protected waters, the master shall ensure that the vessel's exposed cargo hatches and other openings in the hull are...

  13. 29 CFR 1918.41 - Coaming clearances.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.41 Coaming... five feet (1.52 m) high is stowed within three feet (.91 m) of the hatch coaming and employees handling hatch beams and hatch covers are not protected by a coaming at least 24-inch (.61 m) high, a taut...

  14. 46 CFR 185.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

  15. 46 CFR 131.893 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Watertight doors and watertight hatches. 131.893 Section... hatches. Each watertight door in a bulkhead that must be watertight in compliance with the requirements in part 174 of this chapter, and each watertight hatch, must be marked on both sides in letters at least...

  16. 9 CFR 91.29 - Hatches.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

  17. 46 CFR 122.330 - Hatches and other openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Hatches and other openings. 122.330 Section 122.330... Miscellaneous Operating Requirements § 122.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or river routes in calm weather, all hatches and openings in the hull, except loading...

  18. 46 CFR 169.745 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Escape hatches and emergency exits. 169.745 Section 169... VESSELS Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.745 Escape hatches and emergency exits. Each escape hatch and other emergency exit must be marked on both sides using at least 1...

  19. 29 CFR 1918.41 - Coaming clearances.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.41 Coaming... five feet (1.52 m) high is stowed within three feet (.91 m) of the hatch coaming and employees handling hatch beams and hatch covers are not protected by a coaming at least 24-inch (.61 m) high, a taut...

  20. 46 CFR 131.520 - Hatches and other openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Hatches and other openings. 131.520 Section 131.520..., Drills, and Inspections § 131.520 Hatches and other openings. Before any vessel leaves protected waters, the master shall ensure that the vessel's exposed cargo hatches and other openings in the hull are...

  1. 29 CFR 780.211 - Contract production of hatching eggs.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Contract production of hatching eggs. 780.211 Section 780... Agriculture as It Relates to Specific Situations Hatchery Operations § 780.211 Contract production of hatching... the production of hatching eggs which the hatchery agrees to buy. Ordinarily, the farmer furnishes the...

  2. 29 CFR 1918.43 - Handling hatch beams and covers.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 7 2012-07-01 2012-07-01 false Handling hatch beams and covers. 1918.43 Section 1918.43..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.43 Handling hatch beams and covers. Paragraphs (f)(2), (g), and (h) of this section apply only to...

  3. 29 CFR 1918.43 - Handling hatch beams and covers.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 7 2014-07-01 2014-07-01 false Handling hatch beams and covers. 1918.43 Section 1918.43..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.43 Handling hatch beams and covers. Paragraphs (f)(2), (g), and (h) of this section apply only to...

  4. 29 CFR 780.211 - Contract production of hatching eggs.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 3 2012-07-01 2012-07-01 false Contract production of hatching eggs. 780.211 Section 780... Agriculture as It Relates to Specific Situations Hatchery Operations § 780.211 Contract production of hatching... the production of hatching eggs which the hatchery agrees to buy. Ordinarily, the farmer furnishes the...

  5. 46 CFR 185.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

  6. 9 CFR 91.29 - Hatches.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

  7. 46 CFR 131.893 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Watertight doors and watertight hatches. 131.893 Section... hatches. Each watertight door in a bulkhead that must be watertight in compliance with the requirements in part 174 of this chapter, and each watertight hatch, must be marked on both sides in letters at least...

  8. 29 CFR 1918.41 - Coaming clearances.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.41 Coaming... five feet (1.52 m) high is stowed within three feet (.91 m) of the hatch coaming and employees handling hatch beams and hatch covers are not protected by a coaming at least 24-inch (.61 m) high, a taut...

  9. 46 CFR 169.745 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Escape hatches and emergency exits. 169.745 Section 169... VESSELS Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.745 Escape hatches and emergency exits. Each escape hatch and other emergency exit must be marked on both sides using at least 1...

  10. 46 CFR 185.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

  11. 46 CFR 185.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Watertight doors and watertight hatches. 185.610 Section... (UNDER 100 GROSS TONS) OPERATIONS Markings Required § 185.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25...

  12. 46 CFR 131.520 - Hatches and other openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Hatches and other openings. 131.520 Section 131.520..., Drills, and Inspections § 131.520 Hatches and other openings. Before any vessel leaves protected waters, the master shall ensure that the vessel's exposed cargo hatches and other openings in the hull are...

  13. 29 CFR 780.211 - Contract production of hatching eggs.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 3 2013-07-01 2013-07-01 false Contract production of hatching eggs. 780.211 Section 780... Agriculture as It Relates to Specific Situations Hatchery Operations § 780.211 Contract production of hatching... the production of hatching eggs which the hatchery agrees to buy. Ordinarily, the farmer furnishes the...

  14. 46 CFR 185.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

  15. 46 CFR 169.745 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Escape hatches and emergency exits. 169.745 Section 169... VESSELS Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.745 Escape hatches and emergency exits. Each escape hatch and other emergency exit must be marked on both sides using at least 1...

  16. 46 CFR 122.330 - Hatches and other openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Hatches and other openings. 122.330 Section 122.330... Miscellaneous Operating Requirements § 122.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or river routes in calm weather, all hatches and openings in the hull, except loading...

  17. 29 CFR 1918.43 - Handling hatch beams and covers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 7 2011-07-01 2011-07-01 false Handling hatch beams and covers. 1918.43 Section 1918.43..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.43 Handling hatch beams and covers. Paragraphs (f)(2), (g), and (h) of this section apply only to...

  18. 46 CFR 131.520 - Hatches and other openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Hatches and other openings. 131.520 Section 131.520..., Drills, and Inspections § 131.520 Hatches and other openings. Before any vessel leaves protected waters, the master shall ensure that the vessel's exposed cargo hatches and other openings in the hull are...

  19. 29 CFR 1918.43 - Handling hatch beams and covers.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 7 2013-07-01 2013-07-01 false Handling hatch beams and covers. 1918.43 Section 1918.43..., DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Opening and Closing Hatches § 1918.43 Handling hatch beams and covers. Paragraphs (f)(2), (g), and (h) of this section apply only to...

  20. 46 CFR 185.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Watertight doors and watertight hatches. 185.610 Section... (UNDER 100 GROSS TONS) OPERATIONS Markings Required § 185.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25...

  1. 9 CFR 91.29 - Hatches.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

  2. 46 CFR 169.745 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Escape hatches and emergency exits. 169.745 Section 169... VESSELS Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.745 Escape hatches and emergency exits. Each escape hatch and other emergency exit must be marked on both sides using at least 1...

  3. 46 CFR 131.520 - Hatches and other openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Hatches and other openings. 131.520 Section 131.520..., Drills, and Inspections § 131.520 Hatches and other openings. Before any vessel leaves protected waters, the master shall ensure that the vessel's exposed cargo hatches and other openings in the hull are...

  4. 46 CFR 131.893 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Watertight doors and watertight hatches. 131.893 Section... hatches. Each watertight door in a bulkhead that must be watertight in compliance with the requirements in part 174 of this chapter, and each watertight hatch, must be marked on both sides in letters at least...

  5. 46 CFR 122.330 - Hatches and other openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Hatches and other openings. 122.330 Section 122.330... Miscellaneous Operating Requirements § 122.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or river routes in calm weather, all hatches and openings in the hull, except loading...

  6. 123. Pre1911. View forward from near mizzen hatch, starboard side ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    123. Pre-1911. View forward from near mizzen hatch, starboard side showing crew standing on a load of lumber. Note main fife rail, small hatch with cover (possibly original 'lime juice hatch') just aft. Fred Heick Collection. - Ship BALCLUTHA, 2905 Hyde Street Pier, San Francisco, San Francisco County, CA

  7. 46 CFR 185.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Watertight doors and watertight hatches. 185.610 Section... (UNDER 100 GROSS TONS) OPERATIONS Markings Required § 185.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25...

  8. 46 CFR 122.330 - Hatches and other openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Hatches and other openings. 122.330 Section 122.330... Miscellaneous Operating Requirements § 122.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or river routes in calm weather, all hatches and openings in the hull, except loading...

  9. 46 CFR 185.610 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Watertight doors and watertight hatches. 185.610 Section... (UNDER 100 GROSS TONS) OPERATIONS Markings Required § 185.610 Watertight doors and watertight hatches. Watertight doors and watertight hatches must be marked on both sides in clearly legible letters at least 25...

  10. 9 CFR 91.29 - Hatches.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Hatches. 91.29 Section 91.29 Animals... LIVESTOCK FOR EXPORTATION Inspection of Vessels and Accommodations § 91.29 Hatches. (a) Animals may be placed on hatches on exposed decks on an ocean vessel if the pens or stalls are securely lashed down. (b...

  11. 46 CFR 131.893 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Watertight doors and watertight hatches. 131.893 Section... hatches. Each watertight door in a bulkhead that must be watertight in compliance with the requirements in part 174 of this chapter, and each watertight hatch, must be marked on both sides in letters at least...

  12. 46 CFR 169.745 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Escape hatches and emergency exits. 169.745 Section 169... VESSELS Vessel Control, Miscellaneous Systems, and Equipment Markings § 169.745 Escape hatches and emergency exits. Each escape hatch and other emergency exit must be marked on both sides using at least 1...

  13. 46 CFR 185.606 - Escape hatches and emergency exits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

  14. 46 CFR 122.330 - Hatches and other openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Hatches and other openings. 122.330 Section 122.330... Miscellaneous Operating Requirements § 122.330 Hatches and other openings. (a) Except when operating on lakes, bays, and sounds, or river routes in calm weather, all hatches and openings in the hull, except loading...

  15. 46 CFR 131.893 - Watertight doors and watertight hatches.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Watertight doors and watertight hatches. 131.893 Section... hatches. Each watertight door in a bulkhead that must be watertight in compliance with the requirements in part 174 of this chapter, and each watertight hatch, must be marked on both sides in letters at least...

  16. Can-out hatch assembly with magnetic retention means

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Frank, R.C.; Hoh, J.C.

    1985-07-03

    A can-out hatch assembly may be positioned in sealed engagement about aperture within a chamber and is adapted to engage a cover on a container positioned over the aperture to allow the transfer of a contaminant from the chamber to the container while maintaining the contaminant as well as internal portions of the chamber and container isolated from the surrounding environment. With the container's cover engaged by the can-out hatch assembly, the hatch assembly as well as the cover may be pivotally displaced from the aperture with the cover maintaining the exterior portion of the hatch assembly isolated from themore » contaminant. After the contaminant is transferred from the chamber to the container, the hatch assembly and cover are again positioned in sealed engagement about the aperture. The hatch assembly then positions the cover upon the open end of the container in a sealed manner allowing the container to be removed while maintaining the chamber sealed relative to the surrounding environment. The can-out hatch assembly is particularly adapted for operation by remote control means within the sealed chamber.« less

  17. Usefulness of HATCH score as a predictor of atrial fibrillation after coronary artery bypass graft.

    PubMed

    Emren, Volkan; Aldemir, Mustafa; Duygu, Hamza; Kocabaş, Uğur; Tecer, Evren; Cerit, Levent; Erdil, Nevzat

    2016-01-01

    Atrial fibrillation (AF) after coronary artery bypass graft (CABG) surgery is associated with increased morbidity and mortality. The HATCH score was originally devised to predict the progression of paroxysmal AF to persistent AF. To determine whether the HATCH score predicts the development of AF after CABG surgery. The medical records of 284 consecutive patients, who underwent CABG surgery between January 2013 and December 2014, were retrospectively reviewed for the development of AF in the postoperative (POAF) period. The HATCH score, and clinical and echocardiographic parameters were evaluated for all patients. Seventy (25%) patients developed POAF. The HATCH scores were higher in the POAF group (2.8 ± 1.8 vs. 1.1 ± 1.2, p < 0.001). The area of the HATCH score under the curve in the receiver operating characteristics analysis was 773 (95% CI 706-841, p < 0.001). When the HATCH score was 2 or more as a threshold, there was for POAF 72% sensitivity and 75% specificity. The results of the present study suggest that the HATCH score can be used to predict the development of POAF.

  18. Can-out hatch assembly with magnetic retention means

    DOEpatents

    Frank, R.C.; Hoh, J.C.

    1985-07-03

    A can-out hatch assembly may be positioned in sealed engagement about aperture within a chamber and is adapted to engage a cover on a container positioned over the aperture to allow the transfer of a contaminant from the chamber to the container while maintaining the contaminant as well as internal portions of the chamber and container isolated from the surrounding environment. With the container's cover engaged by the can-out hatch assembly, the hatch assembly as well as the cover may be pivotally displaced from the aperture with the cover maintaining the exterior portion of the hatch assembly isolated from the contaminant. After the contaminant is transferred from the chamber to the container, the hatch assembly and cover are again positioned in sealed engagement about the aperture. The hatch assembly then positions the cover upon the open end of the container in a sealed manner allowing the container to be removed while maintaining the chamber sealed relative to the surrounding environment. The can-out hatch assembly is particularly adapted for operation by remote control means within the sealed chamber.

  19. Can-out hatch assembly with magnetic retention means

    DOEpatents

    Frank, Robert C.; Hoh, Joseph C.

    1986-01-07

    A can-out hatch assembly may be positioned in sealed engagement about an aperture within a chamber and is adapted to engage a cover on a container positioned over the aperture to allow the transfer of a contaminant from the chamber to the container while maintaining the contaminant as well as internal portions of the chamber and container isolated from the surrounding environment. With the container's cover engaged by the can-out hatch assembly, the hatch assembly as well as the cover may be pivotally displaced from the aperture with the cover maintaining the exterior portion of the hatch assembly isolated from the contaminant. After the contaminant is transferred from the chamber to the container, the hatch assembly and cover are again positioned in sealed engagement about the aperture. The hatch assembly then positions the cover upon the open end of the container in a sealed manner allowing the container to be removed while maintaining the chamber sealed relative to the surrounding environment. The can-out hatch assembly is particularly adapted for operation by remote control means within the sealed chamber.

  20. Can-out hatch assembly with magnetic retention means

    DOEpatents

    Frank, Robert C.; Hoh, Joseph C.

    1986-01-01

    A can-out hatch assembly may be positioned in sealed engagement about an aperture within a chamber and is adapted to engage a cover on a container positioned over the aperture to allow the transfer of a contaminant from the chamber to the container while maintaining the contaminant as well as internal portions of the chamber and container isolated from the surrounding environment. With the container's cover engaged by the can-out hatch assembly, the hatch assembly as well as the cover may be pivotally displaced from the aperture with the cover maintaining the exterior portion of the hatch assembly isolated from the contaminant. After the contaminant is transferred from the chamber to the container, the hatch assembly and cover are again positioned in sealed engagement about the aperture. The hatch assembly then positions the cover upon the open end of the container in a sealed manner allowing the container to be removed while maintaining the chamber sealed relative to the surrounding environment. The can-out hatch assembly is particularly adapted for operation by remote control means within the sealed chamber.

  1. In ovo delivery of Toll-like receptor 2 ligand, lipoteichoic acid induces pro-inflammatory mediators reducing post-hatch infectious laryngotracheitis virus infection.

    PubMed

    Thapa, S; Nagy, E; Abdul-Careem, M F

    2015-04-15

    Toll-like receptor (TLR) ligands are pathogen associated molecular patterns (PAMPs) recognized by the TLRs resulting in induction of host innate immune responses. One of the PAMPs that binds to TLR2 and cluster of differentiation (CD) 14 is lipotechoic acid (LTA), which activates downstream signals culminating in the release of pro-inflammatory cytokines. In this study, we investigated whether in ovo LTA delivery leads to the induction of antiviral responses against post-hatch infectious laryngotracheitis virus (ILTV) infection. We first delivered the LTA into embryo day (ED)18 eggs via in ovo route so that the compound is available at the respiratory mucosa. Then the LTA treated and control ED18 eggs were allowed to hatch and the hatched chicken was infected with ILTV intratracheally on the day of hatch. We found that in ovo delivered LTA reduces ILTV infection post-hatch. We also found that in ovo delivery of LTA significantly increases mRNA expression of pro-inflammatory mediators in pre-hatch embryo lungs as well as mononuclear cell infiltration, predominantly macrophages, in lung of post-hatch chickens. Altogether, the data suggest that in ovo delivered LTA could be used to reduce ILTV infection in newly hatched chickens. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Interactions between hatch dates, growth rates, and mortality of Age-0 native Rainbow Smelt and nonnative Alewife in Lake Champlain

    USGS Publications Warehouse

    Parrish, Donna; Simonin, Paul W.; Rudstam, Lars G.; Pientka, Bernard; Sullivan, Patrick J.

    2016-01-01

    Timing of hatch in fish populations can be critical for first-year survival and, therefore, year-class strength and subsequent species interactions. We compared hatch timing, growth rates, and subsequent mortality of age-0 Rainbow Smelt Osmerus mordax and Alewife Alosa pseudoharengus, two common open-water fish species of northern North America. In our study site, Lake Champlain, Rainbow Smelt hatched (beginning May 26) almost a month earlier than Alewives (June 20). Abundance in the sampling area was highest in July for age-0 Rainbow Smelt and August for age-0 Alewives. Late-hatching individuals of both species grew faster than those hatching earlier (0.6 mm/d versus 0.4 for Rainbow Smelt; 0.7 mm/d versus 0.6 for Alewives). Mean mortality rate during the first 45 d of life was 3.4%/d for age-0 Rainbow Smelt and was 5.5%/d for age-0 Alewives. Alewife mortality rates did not differ with hatch timing but daily mortality rates of Rainbow Smelt were highest for early-hatching fish. Cannibalism is probably the primary mortality source for age-0 Rainbow Smelt in this lake. Therefore, hatching earlier may not be advantageous because the overlap of adult and age-0 Rainbow Smelt is highest earlier in the season. However, Alewives, first documented in Lake Champlain in 2003, may increase the mortality of age-0 Rainbow Smelt in the summer, which should favor selection for earlier hatching.

  3. Prevalence of atrial fibrillation and the HATCH score: Intensified monitoring of patients with high HATCH score.

    PubMed

    Tischer, Tina S; Schneider, Ralph; Lauschke, Jörg; Diedrich, Doreen; Kundt, Günther; Bänsch, Dietmar

    2015-08-01

    The HATCH score [hypertension, age > 75 years, previous transient ischemic attack (TIA) or stroke (doubled), chronic obstructive pulmonary disease, heart failure (doubled)] has been established to identify patients who are at risk of developing persistent forms of AF. We investigated whether this score is associated with the prevalence of AF in order to guide diagnostic efforts and therapy. The data of 150,408 consecutive patients who were hospitalized at the University Hospital of Rostock between 2007 and 2012 were analyzed. Factors constituting the HATCH score and the presence of AF were prospectively documented using ICD-10 admission codes. Patients were 67.6 ± 13.6 years of age with a mean HATCH score of 1.48 ± 1.02; 16 % had a history of AF and 4 % suffered a TIA or stroke. The prevalence of AF increased significantly with the HATCH score up to 60.0 % (p < 0.001). In all, 63 % of the patients had a HATCH score of 0 and 1 without any history of stroke. The HATCH score correlates with the occurrence of AF, since the prevalence of AF rises with rising score values. Therefore, the HATCH score may be used to select patients for intensified ECG monitoring. Moreover, the score may also be used for stroke risk assessment, as none of the patients with a low HATCH score suffered a stroke.

  4. Staggered larval time-to-hatch and insecticide resistance in the major malaria vector Anopheles gambiae S form.

    PubMed

    Kaiser, Maria L; Koekemoer, Lizette L; Coetzee, Maureen; Hunt, Richard H; Brooke, Basil D

    2010-12-14

    Anopheles gambiae is a major vector of malaria in the West African region. Resistance to multiple insecticides has been recorded in An. gambiae S form in the Ahafo region of Ghana. A laboratory population (GAH) established using wild material from this locality has enabled a mechanistic characterization of each resistance phenotype as well as an analysis of another adaptive characteristic - staggered larval time-to-hatch. Individual egg batches obtained from wild caught females collected from Ghana and the Republic of the Congo were monitored for staggered larval time-to-hatch. In addition, early and late larval time-to-hatch sub-colonies were selected from GAH. These selected sub-colonies were cross-mated and their hybrid progeny were subsequently intercrossed and back-crossed to the parental strains. The insecticide susceptibilities of the GAH base colony and the time-to-hatch selected sub-colonies were quantified for four insecticide classes using insecticide bioassays. Resistance phenotypes were mechanistically characterized using insecticide-synergist bioassays and diagnostic molecular assays for known reduced target-site sensitivity mutations. Anopheles gambiae GAH showed varying levels of resistance to all insecticide classes. Metabolic detoxification and reduced target-site sensitivity mechanisms were implicated. Most wild-caught families showed staggered larval time-to-hatch. However, some families were either exclusively early hatching or late hatching. Most GAH larvae hatched early but many egg batches contained a proportion of late hatching larvae. Crosses between the time-to-hatch selected sub-colonies yielded ambiguous results that did not fit any hypothetical models based on single-locus Mendelian inheritance. There was significant variation in the expression of insecticide resistance between the time-to-hatch phenotypes. An adaptive response to the presence of multiple insecticide classes necessarily involves the development of multiple resistance mechanisms whose effectiveness may be enhanced by intra-population variation in the expression of resistance phenotypes. The variation in the expression of insecticide resistance in association with selection for larval time-to-hatch may induce this kind of enhanced adaptive plasticity as a consequence of pleiotropy, whereby mosquitoes are able to complete their aquatic life stages in a variable breeding environment using staggered larval time-to-hatch, giving rise to an adult population with enhanced variation in the expression of insecticide resistance.

  5. 46 CFR 35.30-10 - Cargo tank hatches, ullage holes, and Butterworth plates-TB/ALL.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Cargo tank hatches, ullage holes, and Butterworth plates... OPERATIONS General Safety Rules § 35.30-10 Cargo tank hatches, ullage holes, and Butterworth plates—TB/ALL. No cargo tank hatches, ullage holes, or Butterworth plates shall be opened or shall remain open...

  6. 46 CFR 35.30-10 - Cargo tank hatches, ullage holes, and Butterworth plates-TB/ALL.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Cargo tank hatches, ullage holes, and Butterworth plates... OPERATIONS General Safety Rules § 35.30-10 Cargo tank hatches, ullage holes, and Butterworth plates—TB/ALL. No cargo tank hatches, ullage holes, or Butterworth plates shall be opened or shall remain open...

  7. 46 CFR 78.17-35 - Hatches and other openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 3 2012-10-01 2012-10-01 false Hatches and other openings. 78.17-35 Section 78.17-35..., Drills, and Inspections § 78.17-35 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself before leaving protected waters that all exposed cargo hatches of his vessel are...

  8. 46 CFR 35.30-10 - Cargo tank hatches, ullage holes, and Butterworth plates-TB/ALL.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo tank hatches, ullage holes, and Butterworth plates... OPERATIONS General Safety Rules § 35.30-10 Cargo tank hatches, ullage holes, and Butterworth plates—TB/ALL. No cargo tank hatches, ullage holes, or Butterworth plates shall be opened or shall remain open...

  9. 46 CFR 35.30-10 - Cargo tank hatches, ullage holes, and Butterworth plates-TB/ALL.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Cargo tank hatches, ullage holes, and Butterworth plates... OPERATIONS General Safety Rules § 35.30-10 Cargo tank hatches, ullage holes, and Butterworth plates—TB/ALL. No cargo tank hatches, ullage holes, or Butterworth plates shall be opened or shall remain open...

  10. 46 CFR 196.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Hatches and other openings. 196.15-20 Section 196.15-20... Test, Drills, and Inspections § 196.15-20 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself that all exposed hatches and other openings in the hull of his...

  11. 78 FR 66825 - Political Activity-Federal Employees Residing in Designated Localities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-07

    ... determination that the District of Columbia meets the criteria in the Hatch Act, as amended by the Hatch Act... Personnel Management, (202) 606-1700. SUPPLEMENTARY INFORMATION: The Hatch Act, at 5 U.S.C. 7323(a)(2) and...-3650. Requests for Hatch Act advisory opinions may be made by email to: [email protected] . The District...

  12. 46 CFR 97.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Hatches and other openings. 97.15-20 Section 97.15-20... OPERATIONS Tests, Drills, and Inspections § 97.15-20 Hatches and other openings. (a)(1) With the exception... himself that all exposed cargo hatches and other openings in the hull of his vessel are closed, made...

  13. 46 CFR 196.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Hatches and other openings. 196.15-20 Section 196.15-20... Test, Drills, and Inspections § 196.15-20 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself that all exposed hatches and other openings in the hull of his...

  14. 46 CFR 97.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Hatches and other openings. 97.15-20 Section 97.15-20... OPERATIONS Tests, Drills, and Inspections § 97.15-20 Hatches and other openings. (a)(1) With the exception... himself that all exposed cargo hatches and other openings in the hull of his vessel are closed, made...

  15. 46 CFR 196.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Hatches and other openings. 196.15-20 Section 196.15-20... Test, Drills, and Inspections § 196.15-20 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself that all exposed hatches and other openings in the hull of his...

  16. 46 CFR 97.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Hatches and other openings. 97.15-20 Section 97.15-20... OPERATIONS Tests, Drills, and Inspections § 97.15-20 Hatches and other openings. (a)(1) With the exception... himself that all exposed cargo hatches and other openings in the hull of his vessel are closed, made...

  17. 46 CFR 78.17-35 - Hatches and other openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Hatches and other openings. 78.17-35 Section 78.17-35..., Drills, and Inspections § 78.17-35 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself before leaving protected waters that all exposed cargo hatches of his vessel are...

  18. 46 CFR 97.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Hatches and other openings. 97.15-20 Section 97.15-20... OPERATIONS Tests, Drills, and Inspections § 97.15-20 Hatches and other openings. (a)(1) With the exception... himself that all exposed cargo hatches and other openings in the hull of his vessel are closed, made...

  19. 46 CFR 97.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Hatches and other openings. 97.15-20 Section 97.15-20... OPERATIONS Tests, Drills, and Inspections § 97.15-20 Hatches and other openings. (a)(1) With the exception... himself that all exposed cargo hatches and other openings in the hull of his vessel are closed, made...

  20. 46 CFR 196.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Hatches and other openings. 196.15-20 Section 196.15-20... Test, Drills, and Inspections § 196.15-20 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself that all exposed hatches and other openings in the hull of his...

  1. 46 CFR 78.17-35 - Hatches and other openings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 3 2011-10-01 2011-10-01 false Hatches and other openings. 78.17-35 Section 78.17-35..., Drills, and Inspections § 78.17-35 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself before leaving protected waters that all exposed cargo hatches of his vessel are...

  2. 46 CFR 196.15-20 - Hatches and other openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Hatches and other openings. 196.15-20 Section 196.15-20... Test, Drills, and Inspections § 196.15-20 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself that all exposed hatches and other openings in the hull of his...

  3. 46 CFR 78.17-35 - Hatches and other openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 3 2014-10-01 2014-10-01 false Hatches and other openings. 78.17-35 Section 78.17-35..., Drills, and Inspections § 78.17-35 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself before leaving protected waters that all exposed cargo hatches of his vessel are...

  4. 46 CFR 35.30-10 - Cargo tank hatches, ullage holes, and Butterworth plates-TB/ALL.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Cargo tank hatches, ullage holes, and Butterworth plates... OPERATIONS General Safety Rules § 35.30-10 Cargo tank hatches, ullage holes, and Butterworth plates—TB/ALL. No cargo tank hatches, ullage holes, or Butterworth plates shall be opened or shall remain open...

  5. 46 CFR 78.17-35 - Hatches and other openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 3 2013-10-01 2013-10-01 false Hatches and other openings. 78.17-35 Section 78.17-35..., Drills, and Inspections § 78.17-35 Hatches and other openings. (a) It shall be the responsibility of the master to assure himself before leaving protected waters that all exposed cargo hatches of his vessel are...

  6. 29 CFR 1918.43 - Handling hatch beams and covers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the hatch shall be lashed or pinned back so that it cannot be moved toward the open section. (2... prevent them from falling off the cover before the hatch cover is moved. (j) When a hatch is to be covered... operations, if positive means are taken to prevent employees from walking on the tarpaulin. [62 FR 40202...

  7. Hatch latch mechanism for Spacelab scientific airlock

    NASA Technical Reports Server (NTRS)

    Terhaar, G. R.

    1979-01-01

    The requirements, design tradeoff, design, and performance of the Spacelab scientific airlock hatch latching mechanisms are described. At space side the hatch is closed and held against internal airlock/module pressure by 12 tangential overcenter hooks driven by a driver. At module side the hatch is held by 4 hooks driven by rollers running on a cammed driver.

  8. Seismic margin assessment of the Edwin I. Hatch Nuclear Plant, Unit 1

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Barr, W.T.; Moore, D.P.; Smith, J.E.

    1991-06-01

    This summary presents the results and lessons learned from the seismic margin assessment (SMA) of Unit 1 of the Hatch Nuclear Plant. The primary purpose of this SMA was to assess the practicality of the EPRI SMA methodology on a BWR on a soil site such as Hatch. The major findings from the Hatch SMA are briefly described along with the lessons learned during the project implementation. The experience gained on the Hatch SMA is expected to benefit others in the performance of future SMAs. 12 refs.

  9. Hatching of Meloidogyne incognita Eggs in the Neutral Carbohydrate Fraction of Root Exudates of Gnotobiotically Grown Alfalfa

    PubMed Central

    Hamlen, R. A.; Bloom, J. R.; Lukezic, F. L.

    1973-01-01

    Meloidogyne incognita eggs were hatched in soil sterilized by gamma kradiation and wetted with root exudates from alfalfa plants in different stages of development and subjected to various levels of clipping. Carbohydrate components of the exudates were identified by gas chromatography-mass spectrometry. Although significant stimulation of hatch was detected in exudates of seedling and flowering plants, the practical importance of the increase is doubtful as hatch in distilled water was always greater than 50%. Hatch did not differ among exudate samples from clipped plants. Incubation of eggs in soil moistened with 10⁻⁷ to 10⁻³ M solutions of glucose did not result in increased hatching over that in distilled water. PMID:19319320

  10. Activation of peroxisome proliferators-activated receptor δ (PPARδ) promotes blastocyst hatching in mice.

    PubMed

    Kang, Hee Jung; Hwang, Soo Jin; Yoon, Jung Ah; Jun, Jin Hyun; Lim, Hyunjung Jade; Yoon, Tae Ki; Song, Haengseok

    2011-10-01

    Prostaglandins participate in a variety of female reproductive processes, including ovulation, fertilization, embryo implantation and parturition. In particular, maternal prostacyclin (PGI(2)) is critical for embryo implantation and the action of PGI(2) is not mediated via its G-protein-coupled membrane receptor, IP, but its nuclear receptor, peroxisome-proliferator-activated receptor δ (PPARδ). Recently, several studies have shown that PGI(2) enhances blastocyst development and/or hatching rate in vitro, and subsequently implantation and live birth rates in mice. However, the mechanism by which PGI(2) improves preimplantation embryo development in vitro remains unclear. Using molecular, pharmacologic and genetic approaches, we show that PGI(2)-induced PPARδ activation accelerates blastocyst hatching in mice. mRNAs for PPARδ, retinoid X receptor (heterodimeric partners of PPARδ) and PGI(2) synthase (PGIS) are temporally induced after zygotic gene activation, and their expression reaches maximum levels at the blastocyst stage, suggesting that functional complex of PPARδ can be formed in the blastocyst. Carbaprostacyclin (a stable analogue of PGI(2)) and GW501516 (a PPARδ selective agonist) significantly accelerated blastocyst hatching but did not increase total cell number of cultured blastocysts. Whereas U51605 (a PGIS inhibitor) interfered with blastocyst hatching, GW501516 restored U51605-induced retarded hatching. In contrast to the improvement of blastocyst hatching by PPARδ agonists, PPAR antagonists significantly inhibited blastocyst hatching. Furthermore, deletion of PPARδ at early stages of preimplantation mouse embryos caused delay of blastocyst hatching, but did not impair blastocyst development. Taken together, PGI(2)-induced PPARδ activation accelerates blastocyst hatching in mice.

  11. 46 CFR 39.6007 - Operational requirements for tank barge cleaning-B/ALL.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... of the setting of any pressure relief valve in the cargo tank venting system. (d) Any hatch and/or... hatch and/or fitting opened must be removed in order to allow for maximum airflow. The hatch and/or... setting of any of the barge's vacuum relief valves. (e) “Do Not Close Hatch/Fitting” signs must be...

  12. 46 CFR 39.6007 - Operational requirements for tank barge cleaning-B/ALL.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of the setting of any pressure relief valve in the cargo tank venting system. (d) Any hatch and/or... hatch and/or fitting opened must be removed in order to allow for maximum airflow. The hatch and/or... setting of any of the barge's vacuum relief valves. (e) “Do Not Close Hatch/Fitting” signs must be...

  13. 9 CFR 82.8 - Interstate movement of eggs, other than hatching eggs, from a quarantined area.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... than hatching eggs, from a quarantined area. 82.8 Section 82.8 Animals and Animal Products ANIMAL AND... Disease (END) § 82.8 Interstate movement of eggs, other than hatching eggs, from a quarantined area. (a) Eggs, other than hatching eggs, from birds or poultry from flocks not known to be infected with END may...

  14. 9 CFR 82.8 - Interstate movement of eggs, other than hatching eggs, from a quarantined area.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... than hatching eggs, from a quarantined area. 82.8 Section 82.8 Animals and Animal Products ANIMAL AND... Disease (END) § 82.8 Interstate movement of eggs, other than hatching eggs, from a quarantined area. (a) Eggs, other than hatching eggs, from birds or poultry from flocks not known to be infected with END may...

  15. 76 FR 19744 - Final Tropic to Hatch 138 kV Transmission Line Project Environmental Impact Statement and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-08

    ... DEPARTMENT OF AGRICULTURE Forest Service Final Tropic to Hatch 138 kV Transmission Line Project..., has prepared a Final Environmental Impact Statement (FEIS) for the Tropic to Hatch 138 kV Transmission.... ADDRESSES: Copies of the Tropic to Hatch 138 kV Transmission Line Project FEIS/PMPA for the Grand Staircase...

  16. 50 CFR Figure 1 to Subpart I of... - Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Existing California Area Closures (hatched areas extend to 3 miles offshore; cross-hatched areas extend beyond 3 miles offshore) and Optional..., DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES OFF WEST COAST STATES Coastal Pelagics Fisheries Pt. 660, Subpt...

  17. DEVELOPMENTAL PATTERN AND ADAPTATIONS FOR REPRODUCTION IN NUCELLA CRASSILABRUM AND OTHER MURICACEAN GASTROPODS.

    PubMed

    Gallardo, C S

    1979-12-01

    1. Eggs of Nucella crassilabrum range from 204 to 293 µm in diameter (mean = 240 µm). Only 6.6 to 7.9% are fertile; the remaining are ingested as nurse eggs. 2. Embryos metamorphose before hatching. Pre-hatching time ranges from 55 to 80 days according to seasonal temperature fluctuations. 3. Hatching size varies from 0.82 to 1.3 mm, depending on number of nurse-eggs ingested per embryo (from 3 to 20). Number of fertile embryos per capsule (10 to 122) depends on capsule size. 4. Hatching type and hatching size shown by N. crassilabrum agree with those of other muricaceans living in similar habitat conditions. 5. Pre-hatching time and hatching size data of various muricaceans are analyzed to determine to what extent they influence embryonic mode of nutrition, namely the presence of nurse-eggs or alternatively large and fertile self-sufficient eggs. Provision of nurse-eggs for embryos is of common occurrence among intertidal muricaceans and this mode of nutrition seems to have been favored in such habitats to reduce developmental time. Providing the yolk as nurse-eggs seems also to contribute to a larger hatching size, as suggested by some subtidal muricaceans with such embryo support patterns.

  18. Hatching late in the season requires flexibility in the timing of song learning

    PubMed Central

    Leitner, Stefan; Teichel, Johanna; Ter Maat, Andries; Voigt, Cornelia

    2015-01-01

    Most songbirds learn their songs from adult tutors, who can be their father or other male conspecifics. However, the variables that control song learning in a natural social context are largely unknown. We investigated whether the time of hatching of male domesticated canaries has an impact on their song development and on the neuroendocrine parameters of the song control system. Average age difference between early- and late-hatched males was 50 days with a maximum of 90 days. Song activity of adult tutor males decreased significantly during the breeding season. While early-hatched males were exposed to tutor songs for on average the first 99 days, late-hatched peers heard adult song only during the first 48 days of life. Remarkably, although hatching late in the season negatively affected body condition, no differences between both groups of males were found in song characteristics either in autumn or in the following spring. Similarly, hatching date had no effect on song nucleus size and circulating testosterone levels. Our data suggest that late-hatched males must have undergone accelerated song development. Furthermore, the limited tutor song exposure did not affect adult song organization and song performance. PMID:26311160

  19. Photosensitivity in the circadian hatching rhythm of the carotenoid-depleted silkworm, Bombyx mori.

    PubMed

    Sakamoto, K; Shimizu, I

    1994-01-01

    Silkworms (Bombyx mori) were reared on a carotenoid-deprived artificial diet, and the carotenoid-depleted eggs of the next generation were incubated so that we could observe the effect of the depletion on the circadian rhythm of hatching. The phototactic response curves of newly hatched larvae showed that the visual photosensitivity in ocelli of larvae from the carotenoid-depleted eggs was at least 4 log units lower than that of a carotenoid-rich control group. However, the phase-shift experiment revealed that carotenoid depletion did not reduce the photosensitivity in the hatching rhythm. When the hatching rhythm was generated by exposure to a single light pulse in constant darkness, the first peak in the rhythm of the carotenoid-depleted silkworms occurred significantly earlier than that of the carotenoid-rich group, but the following second peaks of both groups were found at the same time. These results suggest that for the silkworm, carotenoid is not involved in photoreception for the hatching rhythm, but is involved in the timing of hatching.

  20. 49 CFR 231.28 - Box and other house cars with roof hatches built or placed in service after October 1, 1966.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Box and other house cars with roof hatches built... RAILROAD SAFETY APPLIANCE STANDARDS § 231.28 Box and other house cars with roof hatches built or placed in... fifteen (15) inches from edge of roof, except on refrigerator cars where ice hatches prevent, when...

  1. 49 CFR 231.28 - Box and other house cars with roof hatches built or placed in service after October 1, 1966.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Box and other house cars with roof hatches built... RAILROAD SAFETY APPLIANCE STANDARDS § 231.28 Box and other house cars with roof hatches built or placed in... fifteen (15) inches from edge of roof, except on refrigerator cars where ice hatches prevent, when...

  2. Effects of in ovo feeding of l-arginine on breast muscle growth and protein deposition in post-hatch broilers.

    PubMed

    Yu, L L; Gao, T; Zhao, M M; Lv, P A; Zhang, L; Li, J L; Jiang, Y; Gao, F; Zhou, G H

    2018-02-26

    In ovo feeding (IOF) of l-arginine (Arg) can affect growth performance of broilers, but the response of IOF of Arg on breast muscle growth is unclear, and the mechanism involved in protein deposition remains unknown. Hense, this experiment was conducted to evaluate the effects of IOF of Arg on breast muscle growth and protein-deposited signalling in post-hatch broilers. A total of 720 fertile eggs were collected from 34-week-old Arbor Acres breeder hens and distributed to three treatments: (1) non-injected control group; (2) 7.5 g/l (w/v) NaCl diluent-injected control group; (3) 0.6 mg Arg/egg solution-injected group. At 17.5 days of incubation, fertile eggs were injected 0.6 ml solutions into the amnion of the injected groups. Upon hatching, 80 male chicks were randomly assigned to eight replicates of 10 birds each and fed ad libitum for 21 days. The results indicated that IOF of Arg increased relative breast muscle weight compared with those of control groups at hatch, 3-, 7- and 21-day post-hatch (P<0.05). In the Arg-injected group, the plasma total protein and albumen concentrations were higher at 7- and 21-day post-hatch than those of control groups (P<0.05). The alanine aminotransferase activity in Arg group was higher at hatch than that of control groups (P<0.05). The levels of triiodothyronine at four time points and thyroxine hormones at hatch, 7- and 21-day post-hatch in Arg group were higher than those of control groups (P<0.05). In addition, IOF of Arg increased the amino acid concentrations of breast muscle at hatch, 7- and 21-day post-hatch (P<0.05). In ovo feeding of Arg also enhanced mammalian target of rapamycin, ribosomal protein S6 kinase-1 and eIF4E-bindingprotein-1 messenger RNA expression levels at hatch compared with those of control groups (P<0.05). It was concluded that IOF of Arg treatment improved breast muscle growth, which might be associated with the enhancement of protein deposition.

  3. Effects of breeder age and egg weight on morphological changes in the small intestine of chicks during the hatch window.

    PubMed

    Yalçin, S; Izzetoğlu, G T; Aktaş, A

    2013-01-01

    1. The objective of the study was to investigate the effects of breeder age and egg weight on hatching performance and morphological changes in segments of the small intestine of broiler chicks during a 21 h hatch window. 2. Eggs from Ross broiler breeder flocks aged 29 (young) and 48 weeks (old) were classified as light (LE) or heavy (HE) and incubated at the same conditions. At 475 h of incubation, eggs were checked every 3 h to determine time of external pipping and hatching. The first 42 chicks to emerge from each group were weighed and chick length was measured and 14 chicks from each group were sampled to collect residual yolk and intestine segments. The rest of chicks were placed back in the incubator and chick weight and length were measured individually at 9, 15 and 21 h after chicks hatched. At the end of 21 h, 14 chicks from each group were sampled again and the same procedure was followed. 3. The HE chicks pipped and hatched later than LE, regardless of breeder age. From hatch to the end of the hatch window, chick weight, but not yolk-free chick weight, gradually reduced. Relative residual yolk weight of chicks from both egg weights was similar at hatch, however, yolk sac utilisation was higher for LE chicks during the 21 h post-hatch period. At hatch, jejunum and ileum villus development was very similar for HE and LE chicks but greater development was observed for villus area with an increase in the jejunum villus length, width and goblet cell numbers in HE chicks. 4. The longest jejunum villus and the widest duodenum and jejunum villus were obtained for HE chicks from old breeders indicating that HE chicks from old breeders would have a greater surface area for nutrient absorption.

  4. Effects of in ovo administration of L-carnitine on hatchability performance, glycogen status and insulin-like growth factor-1 of broiler chickens.

    PubMed

    Shafey, T M; Al-Batshan, H A; Al-Owaimer, A N; Al-Samawei, K A

    2010-02-01

    1. Eggs from a meat-type breeder flock (Ross) were used in two trials to study the effects of in ovo administration of L-carnitine (carnitine) on hatchability traits (hatchability percentage, embryo deaths, pipped with live or dead embryo), chick weight at hatch as an absolute value (CWT) or expressed as a percentage of egg weight (CWT%), hatching period, glycogen status (liver and pectoral muscle) and plasma insulin-like growth factor-1 (IGF-1) of hatched chicks were investigated. There were 9 treatments with three replicates of each. Treatments were non-injected control (negative control), or injection with sterilised saline (09%, positive control), or sterilised saline with carnitine at 25, 50, 100, 200, 300, 400, and 500 microg/egg. 2. In ovo carnitine treatment increased CWT, CWT%, glycogen in the liver and pectoral muscle, glycogen index and plasma IGF-1 of hatched chicks, and did not influence hatchability traits and hatching period. The glycogen index of hatched chicks of the in ovo carnitine treatments with values (500 > 400 = 300 > 200) was higher than that of the control and in ovo carnitine at 25, 50, and 100 microg/egg treatments. The nature of response to carnitine was cubic for CWT and CWT%, and linear for glycogen in the liver and pectoral muscle, glycogen index of hatched chicks when the negative control or positive control treatment was used as base line. 3. It was concluded that in ovo administration of carnitine at 25-500 microg/egg increased chick weight at hatch and IGF-1, and did not influence hatchability traits and hatching period of eggs. The linear relationship between in ovo administration of carnitine and glycogen status of hatched chicks indicated that increasing in ovo doses improved glycogen status of hatched chicks.

  5. Effect of temperature and salinity on egg hatching and description of the life cycle of Lernanthropus latis (Copepoda: Lernanthropidae) infecting barramundi, Lates calcarifer.

    PubMed

    Brazenor, Alexander K; Hutson, Kate S

    2013-10-01

    The parasite Lernanthropus latis (Copepoda: Lernanthropidae) is a major threat to the sustained mariculture of barramundi, Lates calcarifer (Perciformes: Latidae). We investigated the effect of water temperature and salinity on egg hatching success of L. latis and describe the life cycle for the first time. Wild and sea-caged L. calcarifer examined in tropical north Australia exhibited similar parasite prevalence (range: 80-100%) and mean parasite intensity (range: 3-6), whereas land-based maricultured fish were not infected. Hatching success and time to first and last hatch was determined for a range of water temperature (22, 30, 32 and 34°C) and salinity (0, 11, 22, 35 and 40‰) combinations representing current and predicted climate conditions. There was a significant interaction between water temperature and salinity on the hatching success of L. latis nauplii. Eggs hatched in all temperature treatments, with the greatest hatching success at 30°C and 32°C (98 and 92% success, respectively) in 35‰. Hatching did not occur at 0‰ and was severely reduced at 11‰ (1.6% success). Hatching began within 6h at all water temperatures with >95% of eggs hatched within 30h at 30, 32 and 34°C and within 60h at 22°C. Adult parasites differed from the original description by the presence of the parabasal flagellum, small setae on the legs and caudal rami and minor incongruences regarding morphological measurements. The life cycle of L. latis includes three free living stages and five parasitic stages. Although L. latis exhibits broad environmental tolerance, freshwater can be used as an effective management strategy to break the life cycle in aquaculture. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  6. The role of HATCH score in predicting the success rate of sinus rhythm following electrical cardioversion of atrial fibrillation.

    PubMed

    Emren, Sadık Volkan; Kocabaş, Uğur; Duygu, Hamza; Levent, Fatih; Şimşek, Ersin Çağrı; Yapan Emren, Zeynep; Tülüce, Selcen

    2016-01-01

    The HATCH score predicts the development of persistent and permanent atrial fibrillation (AF) one year after spontaneous or pharmacological conversion to sinus rhythm in patients with AF. However, it remains unknown whether HATCH score predicts short-term success of the procedure at early stages for patients who have undergone electrical cardioversion (EC) for AF. The present study evaluated whether HATCH score predicts short-term success of EC in patients with AF. The study included patients aged 18 years and over, who had undergone EC due to AF lasting less than 12 months, between December 2011 and October 2013. HATCH score was calculated for all patients. The acronym HATCH stands for Hypertension, Age (above 75 years), Transient ischaemic attack or stroke, Chronic obstructive pulmonary disease, and Heart failure. This scoring system awards two points for heart failure and transient ischaemic attack or stroke and one point for the remaining items. The study included 227 patients and short-term EC was successful in 163 of the cases. The mean HATCH scores of the patients who had undergone successful or unsuccessful EC were 1.3 ± 1.4 and 2.9 ± 1.4, respectively (p < 0.001). The area of the HATCH score under the curve in receiver operating characteristics analysis was (AUC) 0.792 (95% CI 0.727-0.857, p < 0.001). A HATCH score of two and above yielded 77% sensitivity, 62% specificity, 56% positive predictive value, and 87% negative predictive value in predicting unsuccessful cardioversion. HATCH score is useful in predicting short-term success of EC at early stages for patients with AF, for whom the use of a rhythm-control strategy is planned.

  7. Hatching Eggs in the Classroom.

    ERIC Educational Resources Information Center

    Smith, Robert W.

    1984-01-01

    This article provides detailed instructions on how to hatch chicken eggs. Sections include: (1) making the incubator; (2) making the brooder; (3) guidelines for hatching eggs; (4) from incubator to brooder; and (5) recommended readings. (JMK)

  8. Abiotic factors influencing embryonic development, egg hatching, and larval orientation in the reindeer warble fly, Hypoderma tarandi.

    PubMed

    Karter, A J; Folstad, I; Anderson, J R

    1992-10-01

    Wild-caught, tethered females of the reindeer warble fly, Hypoderma tarandi (L.) (= Oedemagena tarandi (L.)), (Diptera, Oestridae) were stimulated to oviposit on hairs of a reindeer hide. Newly laid eggs incubated at constant temperatures and relative humidities hatched within 3 days to 2 weeks, depending on the experimental conditions. Over a range of 7-40 degrees C, hatching only occurred between 20 and 37 degrees C. Eggs held at 100% relative humidity had lower hatchability and longer time to hatch relative to eggs held at 77% relative humidity. The average number of degree-days for hatching was 50.35. Between 20 and 33 degrees C there was a temperature-dependent linear trend in developmental rate, and the proportion of eggs hatching was highest, and least variable, at the mid-temperature ranges. The temperature range found in the natural host micro-habitat where H. tarandi commonly affix their eggs (close to the skin at the base of a host hair) was consistent with the experimental temperature treatments that produced the highest hatching rate. Newly emerged larvae displayed positive thermotaxis, while showing no phototaxic or geotaxic behaviour. Results indicate that constraints of the host environment, coupled with temperature-dependent hatching success, may impose a selective pressure on oviposition behaviour.

  9. Prebreeding survival of Roseate Terns Sterna dougallii varies with sex, hatching order and hatching date

    USGS Publications Warehouse

    Nisbet, Ian C.T.; Monticelli, David; Spendelow, Jeffrey A.; Szczys, Patricia

    2016-01-01

    Unequal sex ratios can reduce the productivity of animal populations and are especially prevalent among endangered species. A cohort of 333 Roseate Tern Sterna dougallii chicks at a site where the adult sex ratio was skewed towards females was sexed at hatching and followed through fledging and return to the breeding area, and subsequently during adulthood. The entire regional metapopulation was sampled for returning birds. Prebreeding survival (from fledging to age 3 years) was lower in males than in females, but only among B-chicks (second in hatching order). Prebreeding survival also declined with hatching date. The proportion of females in this cohort increased from 54.6% at hatching to 56.2% at fledging and to an estimated 58.0% among survivors at age 3 years. This was more than sufficient to explain the degree of skew in the sex ratio of the adult population, but changes in this degree of skew during the study period make it difficult to identify the influence of a single cohort of recruits. Many studies of prebreeding survival in other bird species have identified effects of sex, hatching order or hatching date, but no previous study has tested for effects of all three factors simultaneously.

  10. Climate and geographic trends in hatch delay of the treehole mosquito, Aedes triseriatus Say (Diptera: Culicidae).

    PubMed

    Khatchikian, Camilo E; Dennehy, John J; Vitek, Christopher J; Livdahl, Todd

    2009-06-01

    Eggs of Aedes triseriatus mosquitoes are stimulated to hatch when inundated with water, but only a small fraction of eggs from the same batch will hatch for any given stimulus. Similar hatching or germination patterns are observed in desert plants, copepods, rotifers, insects, and many other species. Bet hedging theory suggests that parents stagger offspring emergence into vulnerable life history stages in order to avoid catastrophic reproductive failures. For Ae. triseriatus, a treehole breeding mosquito, immediate hatching of an entire clutch leaves all of the parent's progeny vulnerable to extinction in the event of a severe drought. Natural selection has likely favored parents that pursued a bet hedging strategy where the risk of reproductive failure is distributed over time. Considering treehole mosquitoes, bet hedging theory could be used to predict that hatch delay would be positively correlated with the likelihood of drought. To test this prediction, we collected Ae. triseriatus from habitats that varied widely in mean annual precipitation and exposed them to several hatch stimuli in the laboratory. Here we report that, as predicted, Ae. triseriatus eggs from high precipitation regions showed less hatch delay than areas of low precipitation. This strategy probably allows Ae. triseriatus to cope with the wide variety of climatic conditions that it faces in its extensive geographical range.

  11. 46 CFR 174.220 - Hatches and coamings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... PERTAINING TO SPECIFIC VESSEL TYPES Special Rules Pertaining to Offshore Supply Vessels § 174.220 Hatches and... securing-devices; and (2) Be attached to the hatch frame or coaming by hinges, captive chains, or other...

  12. 46 CFR 174.220 - Hatches and coamings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... PERTAINING TO SPECIFIC VESSEL TYPES Special Rules Pertaining to Offshore Supply Vessels § 174.220 Hatches and... securing-devices; and (2) Be attached to the hatch frame or coaming by hinges, captive chains, or other...

  13. 46 CFR 174.220 - Hatches and coamings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... PERTAINING TO SPECIFIC VESSEL TYPES Special Rules Pertaining to Offshore Supply Vessels § 174.220 Hatches and... securing-devices; and (2) Be attached to the hatch frame or coaming by hinges, captive chains, or other...

  14. 1. VIEW OF THE ENTRANCE TO THE HATCH ADIT (FEATURE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW OF THE ENTRANCE TO THE HATCH ADIT (FEATURE B-28), FACING WEST. (OCTOBER, 1995) - Nevada Lucky Tiger Mill & Mine, Hatch Adit, East slope of Buckskin Mountain, Paradise Valley, Humboldt County, NV

  15. 49 CFR 176.58 - Preparation of the vessel.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... be examined and all residue of previous cargo removed. (b) All decks, gangways, hatches, and cargo... weather deck of a vessel during loading or unloading operations. (d) Hatch beams and hatch covers may not...

  16. 49 CFR 176.58 - Preparation of the vessel.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... be examined and all residue of previous cargo removed. (b) All decks, gangways, hatches, and cargo... weather deck of a vessel during loading or unloading operations. (d) Hatch beams and hatch covers may not...

  17. 49 CFR 176.58 - Preparation of the vessel.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... be examined and all residue of previous cargo removed. (b) All decks, gangways, hatches, and cargo... weather deck of a vessel during loading or unloading operations. (d) Hatch beams and hatch covers may not...

  18. 49 CFR 176.58 - Preparation of the vessel.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... be examined and all residue of previous cargo removed. (b) All decks, gangways, hatches, and cargo... weather deck of a vessel during loading or unloading operations. (d) Hatch beams and hatch covers may not...

  19. 49 CFR 176.58 - Preparation of the vessel.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... be examined and all residue of previous cargo removed. (b) All decks, gangways, hatches, and cargo... weather deck of a vessel during loading or unloading operations. (d) Hatch beams and hatch covers may not...

  20. 46 CFR 174.220 - Hatches and coamings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... PERTAINING TO SPECIFIC VESSEL TYPES Special Rules Pertaining to Offshore Supply Vessels § 174.220 Hatches and... securing-devices; and (2) Be attached to the hatch frame or coaming by hinges, captive chains, or other...

  1. 46 CFR 174.220 - Hatches and coamings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PERTAINING TO SPECIFIC VESSEL TYPES Special Rules Pertaining to Offshore Supply Vessels § 174.220 Hatches and... securing-devices; and (2) Be attached to the hatch frame or coaming by hinges, captive chains, or other...

  2. Tainted resurrection: metal pollution is linked with reduced hatching and high juvenile mortality in Daphnia egg banks.

    PubMed

    Rogalski, Mary A

    2015-05-01

    Many taxa, from plants to zooplankton, produce long-lasting dormant propagules capable of temporal dispersal. In some cases, propagules can persist for decades or even centuries before emerging from seed and egg banks. Despite impressive longevity, relatively little is known about how the chemical environment experienced before or during dormancy affects the fate and performance of individuals. This study examines the hatching rate and developmental success of Daphnia hatched from diapausing eggs isolated from sediments from four lakes that experienced varying levels of metal contamination. Two hundred seventy-three animals were hatched from lake sediments deposited over the past century. Hatching rate was negatively influenced by metal contamination and sediment age. There was a robust positive relationship between sediment metal concentrations and juvenile mortality in Daphnia hatching from those sediments. The negative effect of metals on Daphnia hatching and juvenile survival may stem from metal bioaccumulation, genetic effects, or reduced maternal investment in diapausing embryos. Regardless of the specific mechanism driving this trend, exposure to metals may impose strong selection on Daphnia diapausing egg banks.

  3. 76 FR 41778 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-15

    .... Applicants: Hatch Solar Energy Center I, LLC. Description: Hatch Solar Energy Center I, LLC submits tariff filing per 35.17(b): Amendment to Hatch Solar Energy Center I, LLC's MBR Tariff to be effective 5/26/2011...

  4. Hatch Cover Slides Through Hatch

    NASA Technical Reports Server (NTRS)

    Alton, Charles; Okane, James H.

    1989-01-01

    Hatch cover for pressurized vessel provides tight seal but opened quickly from either side. In opening or closing, cover sweeps out relatively little volume within vessel, so it does not hinder movement of people or objects from vessel to outside or placement of people or objects near hatch. Cover uses internal pressure to create seal when closed. Design of cover eliminates leakage paths, and cover immune to hazards of sudden decompression or jamming when bolts and latches fail.

  5. Age, growth and hatch dates of ingressing larvae and surviving juveniles of Atlantic menhaden Brevoortia tyrannus.

    PubMed

    Lozano, C; Houde, E D; Wingate, R L; Secor, D H

    2012-10-01

    Ages, growth and hatch dates of ingressing Brevoortia tyrannus larvae were determined in a 3 year sampling survey at the mouth of the Chesapeake Bay, U.S.A. To determine if otolith-aged cohorts had variable relative survival, hatch dates of summer-caught young-of-the-year (YOY) juveniles collected throughout the Chesapeake Bay were compared with hatch dates of ingressing larvae. Modal total length of ingressing larvae was similar among years: 28 mm in 2005-2006 and 2007-2008, and 30 mm in 2006-2007. Ages of ingressing larvae ranged from 9 to 96 days post hatch (dph); mean ages were similar among years, but significantly older in 2006-2007 (50 dph) than in 2005-2006 (44 dph) and 2007-2008 (46 dph). Larval growth rates differed among years. Earliest growth, when larvae were offshore (0-20 dph), was faster in 2006-2007 (0·62 mm day(-1)), than in 2005-2006 and 2007-2008 (0·55 mm day(-1) in these years). Subsequently, from 30 to 80 dph, growth was slowest in 2006-2007. Hatch dates of ingressing larvae occurred from September to March and 90% (2007-2008) to 98% (2006-2007) had hatched prior to 31 December. In contrast, most surviving YOY juvenile B. tyrannus had hatched in January to February, suggesting selective mortality of early-hatched individuals, apparently during the overwinter, larval to juvenile transition period. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  6. STS-38 MS Springer climbs through CCT side hatch prior to egress training

    NASA Image and Video Library

    1990-03-05

    STS-38 Mission Specialist (MS) Robert C. Springer, wearing launch and entry suit (LES), climbs through the side hatch of the crew compartment trainer (CCT) located in JSC's Mockup and Integration Laboratory (MAIL) Bldg 9A. Springer will practice emergency egress through the side hatch using the crew escape system (CES) pole (at Springer's left). The inflated safety cushion under Springer will break his fall as he rolls out of the side hatch.

  7. STS-38 MS Springer climbs through CCT side hatch prior to egress training

    NASA Technical Reports Server (NTRS)

    1990-01-01

    STS-38 Mission Specialist (MS) Robert C. Springer, wearing launch and entry suit (LES), climbs through the side hatch of the crew compartment trainer (CCT) located in JSC's Mockup and Integration Laboratory (MAIL) Bldg 9A. Springer will practice emergency egress through the side hatch using the crew escape system (CES) pole (at Springer's left). The inflated safety cushion under Springer will break his fall as he rolls out of the side hatch.

  8. The light cycle controls the hatching rhythm in Bombyx mori via negative feedback loop of the circadian oscillator.

    PubMed

    Tao, Hui; Li, Xue; Qiu, Jian-Feng; Liu, Heng-Jiang; Zhang, Da-Yan; Chu, Feng; Sima, Yanghu; Xu, Shi-Qing

    2017-10-01

    Hatching behavior is a key target in silkworm (Bombyx mori) rearing, especially for the control of Lepidoptera pests. According to previous research, hatching rhythms appear to be controlled by a clock mechanism that restricts or "gates" hatching to a particular time. However, the underlying mechanism remains elusive. Under 12-h light:12-h dark photoperiod (LD) conditions, the transcriptional levels of the chitinase5 (Cht5) and hatching enzyme-like (Hel) genes, as well as the enzymatic activities of their gene products, oscillated in time with ambient light cycles, as did the transcriptional levels of the cryptochrome 1, cryptochrome 2, period (per), and timeless genes, which are key components of the negative feedback loop of the circadian rhythm. These changes were related to the expression profile of the ecdysteroid receptor gene and the hatching behavior of B. mori eggs. However, under continuous light or dark conditions, the hatching behavior, the expression levels of Cht5 and Hel, as well as the enzymatic activities of their gene products, were not synchronized unlike under LD conditions. In addition, immunohistochemistry experiments showed that light promoted the translocation of PER from the cytoplasm to the nucleus. In conclusion, LD cycles regulate the hatching rhythm of B. mori via negative feedback loop of the circadian oscillator. © 2017 Wiley Periodicals, Inc.

  9. Intron-loss evolution of hatching enzyme genes in Teleostei

    PubMed Central

    2010-01-01

    Background Hatching enzyme, belonging to the astacin metallo-protease family, digests egg envelope at embryo hatching. Orthologous genes of the enzyme are found in all vertebrate genomes. Recently, we found that exon-intron structures of the genes were conserved among tetrapods, while the genes of teleosts frequently lost their introns. Occurrence of such intron losses in teleostean hatching enzyme genes is an uncommon evolutionary event, as most eukaryotic genes are generally known to be interrupted by introns and the intron insertion sites are conserved from species to species. Here, we report on extensive studies of the exon-intron structures of teleostean hatching enzyme genes for insight into how and why introns were lost during evolution. Results We investigated the evolutionary pathway of intron-losses in hatching enzyme genes of 27 species of Teleostei. Hatching enzyme genes of basal teleosts are of only one type, which conserves the 9-exon-8-intron structure of an assumed ancestor. On the other hand, otocephalans and euteleosts possess two types of hatching enzyme genes, suggesting a gene duplication event in the common ancestor of otocephalans and euteleosts. The duplicated genes were classified into two clades, clades I and II, based on phylogenetic analysis. In otocephalans and euteleosts, clade I genes developed a phylogeny-specific structure, such as an 8-exon-7-intron, 5-exon-4-intron, 4-exon-3-intron or intron-less structure. In contrast to the clade I genes, the structures of clade II genes were relatively stable in their configuration, and were similar to that of the ancestral genes. Expression analyses revealed that hatching enzyme genes were high-expression genes, when compared to that of housekeeping genes. When expression levels were compared between clade I and II genes, clade I genes tends to be expressed more highly than clade II genes. Conclusions Hatching enzyme genes evolved to lose their introns, and the intron-loss events occurred at the specific points of teleostean phylogeny. We propose that the high-expression hatching enzyme genes frequently lost their introns during the evolution of teleosts, while the low-expression genes maintained the exon-intron structure of the ancestral gene. PMID:20796321

  10. Incubation temperature and time of hatch impact broiler muscle growth and morphology.

    PubMed

    Clark, D L; Walter, K G; Velleman, S G

    2017-09-01

    The adult myogenic population of stem cells, called satellite cells, initially develop in late-term embryos. Satellite cells are the only myogenic cell that repair damaged myofibers and increase post-hatch growth. The objective of the current study was to determine if incubation temperatures and time of hatch impact growth and pectoralis major (p. major) muscle morphology. Eggs were incubated at a constant 37.8°C; however, from d 14 to 18, the eggs were subject to 39.5°C for 0, 3, or 12 h per day. Chicks were divided into early, mid, or late hatch groups based upon the time they emerged from the shell. Growth and feed efficiency were measured throughout the 63-day trial, while meat quality and muscle morphology were evaluated at the time of processing. The chicks incubated at an increased temperature for 12 h per d had reduced (P < 0.01) body weights throughout the trial compared to the 3 h treatment and control. The early hatch broilers were heavier (P < 0.01) at 63 d compared to mid and late hatch broilers. Chicks from the 12 h incubation treatment had an increased (P = 0.01) gain to feed ratio compared to the control. Broilers from the 12 h incubation treatment had lower (P < 0.01) p. major weights compared to the 0 and 3 h treatments. Early hatch broilers had heavier p. major weights (P < 0.01) compared to mid and late hatch groups. The 12 h incubation treatment also reduced the number of broilers with moderate to severe myopathic attributes compared to the control. Similarly, there were fewer late hatch birds with fibrotic and necrotic p. major muscles compared to the early hatch group. Together, these data demonstrate that altering incubation temperature is a feasible management strategy to improve muscle morphology without negatively impacting meat quality parameters. © 2017 Poultry Science Association Inc.

  11. Factors affecting hatch success of hawksbill sea turtles on Long Island, Antigua, West Indies.

    PubMed

    Ditmer, Mark Allan; Stapleton, Seth Patrick

    2012-01-01

    Current understanding of the factors influencing hawksbill sea turtle (Eretmochelys imbricata) hatch success is disparate and based on relatively short-term studies or limited sample sizes. Because global populations of hawksbills are heavily depleted, evaluating the parameters that impact hatch success is important to their conservation and recovery. Here, we use data collected by the Jumby Bay Hawksbill Project (JBHP) to investigate hatch success. The JBHP implements saturation tagging protocols to study a hawksbill rookery in Antigua, West Indies. Habitat data, which reflect the varied nesting beaches, are collected at egg deposition, and nest contents are exhumed and categorized post-emergence. We analyzed hatch success using mixed-model analyses with explanatory and predictive datasets. We incorporated a random effect for turtle identity and evaluated environmental, temporal and individual-based reproductive variables. Hatch success averaged 78.6% (SD: 21.2%) during the study period. Highly supported models included multiple covariates, including distance to vegetation, deposition date, individual intra-seasonal nest number, clutch size, organic content, and sand grain size. Nests located in open sand were predicted to produce 10.4 more viable hatchlings per clutch than nests located >1.5 m into vegetation. For an individual first nesting in early July, the fourth nest of the season yielded 13.2 more viable hatchlings than the initial clutch. Generalized beach section and inter-annual variation were also supported in our explanatory dataset, suggesting that gaps remain in our understanding of hatch success. Our findings illustrate that evaluating hatch success is a complex process, involving multiple environmental and individual variables. Although distance to vegetation and hatch success were inversely related, vegetation is an important component of hawksbill nesting habitat, and a more complete assessment of the impacts of specific vegetation types on hatch success and hatchling sex ratios is needed. Future research should explore the roles of sand structure, nest moisture, and local weather conditions.

  12. An insight into the heat and mass transfer mechanisms of eggshells hatching broiler chicks and its effects to the hatcher environment.

    PubMed

    Romanini, C E B; Exadaktylos, V; Hong, S W; Tong, Q; McGonnell, I; Demmers, T G M; Bergoug, H; Guinebretière, M; Eterradossi, N; Roulston, N; Verhelst, R; Bahr, C; Berckmans, D

    2015-02-01

    Thermodynamic study of incubated eggs is an important component in the optimisation of incubation processes. However, research on the interaction of heat and moisture transfer mechanisms in eggs is rather limited and does not focus on the hatching stage of incubation. During hatch, both the recently hatched chick and the broken eggshell add extra heat and moisture contents to the hatcher environment. In this study, we have proposed a novel way to estimate thermodynamically the amount of water evaporated from a broken eggshell during hatch. The hypothesis of this study considers that previously reported drops in eggshell temperature during hatching of chicks is the result remaining water content evaporating from the eggshell, released on the inner membrane by the recently hatched wet chick, just before hatch. To reproduce this process, water was sprayed on eggshells to mimic the water-fluid from the wet body of a chick. For each sample of eggshell, the shell geometry and weight, surface area and eggshell temperature were measured. Water evaporation losses and convection coefficient were calculated using a novel model approach considering the simultaneous heat and mass transfer profiles in an eggshell. The calculated average convective coefficient was 23.9 ± 7.5 W/m(2) °C, similar to previously reported coefficients in literature as a function of 0.5-1m/s air speed range. Comparison between measured and calculated values for the water evaporation showed 68% probability accuracy, associated to the use of an experimentally derived single heat transfer coefficient. The results support our proposed modelling approach of heat and mass transfer mechanisms. Furthermore, by estimating the amount of evaporated water in an eggshell post-hatch, air humidity levels inside the hatcher can be optimised to ensure wet chicks dry properly while not dehydrating early hatching chicks. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. Production traits of artificially and naturally hatched geese in intensive and free-range systems - II: slaughter, carcass and meat quality traits.

    PubMed

    Boz, M A; Sarıca, M; Yamak, U S

    2017-04-01

    1. This study investigates the slaughter, carcass and meat quality traits of artificially and naturally hatched geese in intensive and free-range production systems. 2. The study was conducted with 114 naturally hatched and 102 artificially hatched geese. From each replicate of the intensive and free-range systems, one female and one male goose were slaughtered at the ages of 14, 16 and 18 weeks (a total of 32 geese per slaughter week). 3. Artificially hatched geese had higher slaughter weights (5280 vs. 4404 g), carcass weights (3520 vs. 2863), dressing percentages (66.6-65.2% vs. 65.0-63.6%) and carcass part, feather and edible inner organ weights. The ratio of both edible inner organs and abdominal fat was higher in naturally hatched geese. Breast meat L*, a* and pH values and thigh meat dry matter values were higher in artificially hatched geese, whereas thigh meat b* and pH values were higher in naturally hatched geese. 4. Intensively reared geese had higher slaughter weights (4900 vs. 4783 g), carcass weights (3253 vs. 3130 g) and abdominal fat weights (280 vs. 250 g), as well as higher dressing percentages (66.3-64.9% vs. 65.3-63.9%). Breast meat b* and thigh meat L* values were higher in the intensive system, while breast and thigh pH values, dripping loss and cooking loss were higher in the free-range system. Water-holding capacity was higher in the intensive system. 5. In conclusion, artificially hatched, intensively reared geese had the highest slaughter weights; however, both artificially and naturally hatched geese raised in a free-range system reached acceptable slaughter weights and can thus be recommended for use with this type of production system.

  14. Factors Affecting Hatch Success of Hawksbill Sea Turtles on Long Island, Antigua, West Indies

    PubMed Central

    Ditmer, Mark Allan; Stapleton, Seth Patrick

    2012-01-01

    Current understanding of the factors influencing hawksbill sea turtle (Eretmochelys imbricata) hatch success is disparate and based on relatively short-term studies or limited sample sizes. Because global populations of hawksbills are heavily depleted, evaluating the parameters that impact hatch success is important to their conservation and recovery. Here, we use data collected by the Jumby Bay Hawksbill Project (JBHP) to investigate hatch success. The JBHP implements saturation tagging protocols to study a hawksbill rookery in Antigua, West Indies. Habitat data, which reflect the varied nesting beaches, are collected at egg deposition, and nest contents are exhumed and categorized post-emergence. We analyzed hatch success using mixed-model analyses with explanatory and predictive datasets. We incorporated a random effect for turtle identity and evaluated environmental, temporal and individual-based reproductive variables. Hatch success averaged 78.6% (SD: 21.2%) during the study period. Highly supported models included multiple covariates, including distance to vegetation, deposition date, individual intra-seasonal nest number, clutch size, organic content, and sand grain size. Nests located in open sand were predicted to produce 10.4 more viable hatchlings per clutch than nests located >1.5 m into vegetation. For an individual first nesting in early July, the fourth nest of the season yielded 13.2 more viable hatchlings than the initial clutch. Generalized beach section and inter-annual variation were also supported in our explanatory dataset, suggesting that gaps remain in our understanding of hatch success. Our findings illustrate that evaluating hatch success is a complex process, involving multiple environmental and individual variables. Although distance to vegetation and hatch success were inversely related, vegetation is an important component of hawksbill nesting habitat, and a more complete assessment of the impacts of specific vegetation types on hatch success and hatchling sex ratios is needed. Future research should explore the roles of sand structure, nest moisture, and local weather conditions. PMID:22802928

  15. Thermal and Hydraulic Conditions Supporting the Recruitment of Asian Carp in Seiche Affected Rivers of Lake Erie Basin - A Case Study of the Lower Sandusky River in Ohio

    NASA Astrophysics Data System (ADS)

    Soong, D. T.; Santacruz, S.; Jones, L.; Garcia, T.; Kočovský, P. M.; Embke, H.

    2017-12-01

    Grass Carp Ctenopharyngodon idella (Cyprinidae) is an invasive fish species that spawns in rivers during high-flow events. In their native range, it is believed eggs must hatch within the riverine environment in order to eventually result in production of adult fish. The lower Sandusky River is approximately 26 km long extending from its confluence with Sandusky Bay upstream to the Ballville Dam, which is impassible for Grass Carp. Grass Carp are known to have spawned in the Sandusky River, a tributary to Lake Erie, in 2011, 2013, 2015, and 2017. This study characterizes the thermal and hydraulic conditions under which these eggs could hatch in the lower Sandusky River, a relatively short river reach for egg hatching. Grass Carp eggs collected in 2015 were previously analyzed for hatching locations using a one-dimensional steady-state HEC-RAS hydraulic model. In this study we refine estimates of hatching locations by incorporating the influence of fluctuating water levels downstream due to seiches in Lake Erie and overland and tributary inflows using an unsteady 1D/2D HEC-RAS hydraulic model. Additionally, conditions conducive to successful hatching, which occurs when eggs reach the hatching stage within the river, were analyzed from nine high-flow events between 2011 and 2015. Simulated hydraulic and water temperature data were used as inputs to the Fluvial Egg Drift Simulator (FluEgg) model, which was used to analyze the transport and dispersal of Grass carp eggs until hatching. We will describe the differences in steady- and unsteady-state hydraulic modeling in predicting hatching locations of Grass Carp eggs for the 2015 spawning events. Results will also include hydraulic and temperature variables that contribute to the successful/unsuccessful in-river hatching for the nine flow events simulated.

  16. Effects of gamma irradiation on the grape vine moth, Lobesia botrana, eggs

    NASA Astrophysics Data System (ADS)

    Mansour, M.; Al-Attar, J.

    2012-11-01

    Eggs of the grape vine moth, Lobesia botrana (Denis and Schiffermuller), ranging in age from 1-24 to 73-96 h, were exposed, at 24 h intervals, to gamma radiation ranging from 25-600 Gy. The effects of gamma radiation on egg hatch, pupation, adult emergence, sex ratio and rate of development were examined. Results showed that the radiosensitivity of the grape vine moth eggs decreased with increasing age and increased with increasing radiation dose. Egg hatch in 1-24 h old eggs was significantly affected at 25 Gy and completely prevented at 100 Gy. At the age of 25-48 h, radiation sensitivity was only a little lower; egg hatch at 100 Gy was <1% and at 125 Gy no egg hatch was observed. Egg sensitivity to gamma irradiation decreased significantly in the 49-72 h age group; egg hatch was 66% at 100 Gy, and 500 Gy did not completely stop egg hatch (<1%). Eggs irradiated a few hours before egg hatch (73-96 h old) were the most resistant; 150 Gy had no significant effect on egg hatch and at 600 Gy over 33% of the eggs hatched. When pupation or adult emergence was used as a criterion for measuring effectiveness, however, the effects of gamma radiation were very severe. In the most resistant age group (73-96 h old), 150 Gy completely prevented pupation and adult emergence and all larvae resulting from eggs irradiated <49 h old died before pupation. In addition, the rate of development of immature stages resulting from irradiated eggs was negatively affected and sex ratio was skewed in favor of males.

  17. Evaluating the HATCH score for predicting progression to sustained atrial fibrillation in ED patients with new atrial fibrillation.

    PubMed

    Barrett, Tyler W; Self, Wesley H; Wasserman, Brian S; McNaughton, Candace D; Darbar, Dawood

    2013-05-01

    Atrial fibrillation (AF) is often first detected in the emergency department (ED). Not all AF patients progress to sustained AF (ie, episodes lasting >7 days), which is associated with increased morbidity. The HATCH score stratifies patients with paroxysmal AF according to their risk for progression to sustained AF within 1 year. The HATCH score has previously never been tested in ED patients. We evaluated the accuracy of the HATCH score to predict progression to sustained AF within 1 year of initial AF diagnosis in the ED. We conducted a retrospective cohort study of 253 ED patients with new onset AF and known rhythm status for 1 year following the initial AF detection. The exposure variable was the HATCH score at initial ED evaluation. The primary outcome was rhythm status at 1 year following initial AF diagnosis. We constructed a receiver operating characteristic curve and calculated the area under the curve to estimate the HATCH score's accuracy of predicting progression to sustained AF. Overall, 61 (24%) of 253 of patients progressed to sustained AF within 1 year of initial detection, and the HATCH score receiver operating characteristic area under the curve was 0.62 (95% confidence interval, 0.54-0.70). Among ED patients with new onset AF, the HATCH score was a modest predictor of progression to sustained AF. Because only 2 patients had a HATCH greater than 5, this previously recommended cut-point was not useful in identifying high-risk patients in this cohort. Refinement of this decision aid is needed to improve its prognostic accuracy in the ED population. Copyright © 2013 Elsevier Inc. All rights reserved.

  18. 3-D analysis of a containment equipment hatch

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Greimann, L.; Fanous, F.

    1985-01-01

    There are at least two models used to characterize the possible leakage of a containment during a severe accident: (1) the threshold model in which the containment is assumed to be leak-tight until certain pressure/temperature conditions are reached and a very large rupture occurs; and (2) the leak-before-break model in which small leak paths are hypothesized to develop at levels below the threshold. The objective of this work is to investigate the leak-before-break potential of a typical equipment hatch seal. The relative deformations of the sealing surfaces during pressurization are of interest, especially if any buckling of the hatch occurs.more » A three-dimensional finite element model of the equipment hatch assembly was developed. The model included: shell elements for the containment shell, containment stiffeners, penetration sleeve and hatch shell; prestressed bar elements for the swing bolts which hold the hatch closed; and interface elements for the sliding or opening which can occur at the seal surfaces. The nonlinear material properties were approximated by a piecewise linear curve with a proportional limit equal to one-half the yield strength. Geometric nonlinearities were also included in the model. As pressure increments were added to the finite element model, the seal surfaces tended to move together initially. The dominate observable behavior in this range was ''ovaling'' of the penetration sleeve relative to the hatch cover. Since the hatch itself tended to remain circular, there was a mismatch at the sealing surface. Friction reduces but does not eliminate this relative motion. As the containment reached a higher pressure level, the hatch began to buckle at the idealized imperfection. The finite element solution was incremented through the snapthrough. As this postbuckling occurred, additional seal interface distortion was observed.« less

  19. Chilling requirements for hatching of a New Zealand isolate of Nematodirus filicollis.

    PubMed

    Oliver, A-M B; Pomroy, W E; Ganesh, S; Leathwick, D M

    2016-08-15

    The eggs of some species of the parasitic nematode Nematodirus require a period of chilling before they can hatch; N. filicollis is one such species. This study investigated this requirement for chilling in a New Zealand strain of this species. Eggs of N. filicollis were extracted from lamb's faeces and incubated at 20°C to allow development to the third stage larvae within the egg. These eggs were then placed into tissue culture plates and incubated at: 2.7°C (±0.99), 3.6°C (±0.90), 4.7°C (±0.35), 6.4°C (±0.37), 8.0°C (±1.54) or 9.9°C (±0.14) for up to 224 days. At 14day intervals until day 84, then every 28 days, one plate was removed from each temperature and placed at 13.1°C (±0.44) for 14 days. Eggs were then assessed for hatching. From this data, chill units were calculated by subtracting the culture temperature from a constant threshold of 11°C and multiplying by the number of days for which the sample was cultured; then the Gompertz model fitted. Even though hatching overall was low, a greater proportion of eggs hatched with chill accumulation. Maximum hatching of eggs required 800-1000 chill units. Consequently in the field, more than one season of chilling would be required before hatching. As such a generation time could take more than one year to complete. This is different to the hatching dynamics of N. spathiger, the other main species found in New Zealand sheep, which does not display this requirement for chilling and hatches immediately once the third stage larvae are developed. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. Purification and characterization of zebrafish hatching enzyme - an evolutionary aspect of the mechanism of egg envelope digestion.

    PubMed

    Sano, Kaori; Inohaya, Keiji; Kawaguchi, Mari; Yoshizaki, Norio; Iuchi, Ichiro; Yasumasu, Shigeki

    2008-12-01

    There are two hatching enzyme homologues in the zebrafish genome: zebrafish hatching enzyme ZHE1 and ZHE2. Northern blot and RT-PCR analysis revealed that ZHE1 was mainly expressed in pre-hatching embryos, whereas ZHE2 was rarely expressed. This was consistent with the results obtained in an experiment conducted at the protein level, which demonstrated that one kind of hatching enzyme, ZHE1, was able to be purified from the hatching liquid. Therefore, the hatching of zebrafish embryo is performed by a single enzyme, different from the finding that the medaka hatching enzyme is an enzyme system composed of two enzymes, medaka high choriolytic enzyme (MHCE) and medaka low choriolytic enzyme (MLCE), which cooperatively digest the egg envelope. The six ZHE1-cleaving sites were located in the N-terminal regions of egg envelope subunit proteins, ZP2 and ZP3, but not in the internal regions, such as the ZP domains. The digestion manner of ZHE1 appears to be highly analogous to that of MHCE, which partially digests the egg envelope and swells the envelope. The cross-species digestion using enzymes and substrates of zebrafish and medaka revealed that both ZHE1 and MHCE cleaved the same sites of the egg envelope proteins of two species, suggesting that the substrate specificity of ZHE1 is quite similar to that of MHCE. However, MLCE did not show such similarity. Because HCE and LCE are the result of gene duplication in the evolutionary pathway of Teleostei, the present study suggests that ZHE1 and MHCE maintain the character of an ancestral hatching enzyme, and that MLCE acquires a new function, such as promoting the complete digestion of the egg envelope swollen by MHCE.

  1. Some technical and ecological determinants of hatchability in Aedes albopictus, a potential candidate for transposon-mediated transgenesis.

    PubMed

    Dieng, Hamady; Boots, Michael; Tamori, Naoki; Higashihara, Junko; Okada, Takashi; Kato, Kotaro; Eshita, Yuki

    2006-09-01

    Fertility is a physiological process of great importance underlying the dynamics of mosquito populations. In transgenesis, it is a prerequisite for the production of subsequent generations and a crucial parameter for evaluating efficiency. Yet, ongoing success in mosquito vector transformation is being severely affected by low embryo survivability. In the prospect of overcoming this impediment, we investigated the darkening/hardening process of the chorion, the effects of some parameters required for transgenesis on hatch success, and erratic hatching in Aedes albopictus, a species that has not yet been targeted for transformation. The eggs from this species, when placed in a moistened environment while whitish, become dark and yet still remain soft approximately 2 h 10 min postoviposition. Those reared in a high moisture environment hatched at a high rate compared with their counterparts submitted to a drier environment. Submission of eggs to p-nitrophenyl-p'-guanidino-benzoate, a substance known to delay the darkening/delay process, resulted in a hatch rate lower than that from eggs soaked in distilled water, which suggests a negative impact on viability. Heat-shock treatment did not taint embryo viability. Overall, eggs displayed a tolerance to an hour of heat shock at 39 degrees C but still hatched at a considerable rate after a 1 hr exposure to 42 degrees C. Hatching was erratic, with a high rate of hatching on the initial flooding and lower rates of hatching on subsequent floodings, all of which resulted cumulatively in considerable hatch success. Our results should serve as a useful reference for the production of both transgenic and laboratory strains of floodwater Aedes mosquitoes.

  2. Evidence of possible vertical transmission of Tembusu virus in ducks.

    PubMed

    Zhang, Ying; Li, Xiuli; Chen, Hao; Ti, Jinfeng; Yang, Guoping; Zhang, Lu; Lu, Yunjian; Diao, Youxiang

    2015-09-30

    In 2013, Tembusu virus (TMUV) infection was successively observed on several breeding duck farms in Shandong province, China. Affected ducks showed consistently acute anorexia, diarrhea and egg production drop. 125 hatching eggs produced by TMUV infected breeding ducks from four duck farms were collected. Among them, 35 hatching eggs were selected randomly from all before incubation for vitelline membrane samples collection. The rest of 90 hatching eggs were incubated routinely. As a result, 16 hatching eggs were found non-embryonated, 28 duck embryos died during incubation and 46 newly hatched ducklings were obtained. Vitelline membranes of non-embryonated hatching eggs, vitelline membrane, brain or liver samples of dead embryos and brain samples of newly hatched ducklings were collected for virus detection. Samples collected from one egg, embryo or duckling were treated as one. Consequently, 18 of 35 (51.43%) hatching eggs, 2 of 16 (12.50%) non-embryonated duck eggs, 17 of 28 (60.71%) dead duck embryos and 5 of 46 (10.87%) newly hatched ducklings were detected positive for TMUV using NS3-based RT-PCR. Overall, 42 of 125 (33.6%) eggs were positive for TMUV. A virus strain, designated as TMUV-SDDE, was isolated from one of these dead duck embryos which were detected TMUV positive. The results of phylogenetic analysis showed that E gene of TMUV-SDDE virus was closely related to other TMUV strains isolated in China during 2010-2013. Pathogenicity studies showed that TMUV-SDDE strain was virulent to ducklings. This is the first report that TMUV is isolated from duck embryos. The findings provide evidence of possible vertical transmission of TMUV from breeding ducks to ducklings. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Heavier chicks at hatch improves marketing body weight by enhancing skeletal muscle growth.

    PubMed

    Sklan, D; Heifetz, S; Halevy, O

    2003-11-01

    This study examined some of the factors connected with the greater marketing weight observed in chicks hatching with higher BW. Examination of chicks hatching from maternal flocks of different ages indicated that BW at hatch increased quadratically and yolk sac weight linearly with age, whereas components of the gastrointestinal tract showed no significant trend. Growth of pectoralis muscles and gastrointestinal tract were compared in chicks hatching at the same weight from maternal flocks of 28 and 64 wk of age and in chicks from the same maternal flock (44 wk old) hatching at different weights. The results indicated that no differences were found among chicks hatching with the same weight from maternal flocks of different ages. In contrast, in chicks from the same maternal flock hatching at different weights the gastrointestinal tract tended to compose a smaller proportion of BW in large chicks, and its growth was not correlated with performance. Liver proportions were greater in heavier chicks. Pectoralis growth and satellite cell numbers and activity were greater in heavier chicks through 5 d posthatch, and pectoralis muscles were heavier at marketing. Examination of some of the growth factors involved suggested that in heavier chicks satellite cells underwent higher proliferation and earlier differentiation during their critical period of activity in the immediate posthatch days. To determine when these differences in activity were established, examination of 15-d embryonic myoblast activity indicated that at this stage activity was already greater in the heavier eggs. This finding suggests that programming of muscle growth may be completed in late embryonic stages. This study suggests that enhanced satellite cell activity is involved in increased growth of chicks hatching with higher BW.

  4. High Pressure Coolant Injection system risk-based inspection guide for Hatch Nuclear Power Station

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    DiBiasio, A.M.

    1993-05-01

    A review of the operating experience for the High Pressure Coolant Injection (HPCI) system at the Hatch Nuclear Power Station, Units 1 and 2, is described in this report. The information for this review was obtained from Hatch Licensee Event Reports (LERs) that were generated between 1980 and 1992. These LERs have been categorized into 23 failure modes that have been prioritized based on probabilistic risk assessment considerations. In addition, the results of the Hatch operating experience review have been compared with the results of a similar, industry wide operating, experience review. This comparison provides an indication of areas inmore » the Hatch HPCI system that should be given increased attention in the prioritization of inspection resources.« less

  5. Edwin I. Hatch nuclear plant implementation of improved technical specifications

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mahler, S.R.; Pendry, D.

    1994-12-31

    Edwin I. Hatch nuclear plant consists of two General Electric boiling water reactor/4 units, with a common control room and a common refueling floor. In March 1993, Hatch began conversion of both units` technical specifications utilizing NUREG 1433. The technical specifications amendment request was submitted February 25, 1994. Issuance is scheduled for October 21, 1994, with implementation on March 15, 1994. The current unit-1 technical specifications are in the {open_quotes}custom{close_quotes} format, and the unit-2 technical specifications are in the old standard format. Hatch previously relocated the fire protection and radiological technical specifications requirements. The Hatch conversion will provide consistency betweenmore » the two units, to the extent practicable.« less

  6. Boldness and aggressiveness in early and late hatched three-spined sticklebacks Gasterosteus aculeatus.

    PubMed

    Ruiz-Gomez, M L; Huntingford, F A

    2012-08-01

    Levels of boldness and the degree of aggressiveness were compared in juvenile three-spined sticklebacks Gasterosteus aculeatus that had hatched early and late in the breeding season. The most striking result found in this study was that early hatched individuals were bolder when exploring a novel environment than were late-hatched individuals. No differences in levels of aggression between early and late hatchlings were found, but a relationship between boldness and aggressiveness was present regardless of hatching date. The implications of these findings are discussed in the light of research on individual variation in behaviour and the development of behavioural syndromes. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  7. Mechanistic insights into the effect of nanoparticles on zebrafish hatch.

    PubMed

    Ong, Kimberly Jessica; Zhao, Xinxin; Thistle, Maria E; Maccormack, Tyson J; Clark, Rhett J; Ma, Guibin; Martinez-Rubi, Yadienka; Simard, Benoit; Loo, Joachim Say Chye; Veinot, Jonathan G C; Goss, Greg G

    2014-05-01

    Aquatic organisms are susceptible to waterborne nanoparticles (NP) and there is only limited understanding of the mechanisms by which these emerging contaminants may affect biological processes. This study used silicon (nSi), cadmium selenide (nCdSe), silver (nAg) and zinc NPs (nZnO) as well as single-walled carbon nanotubes (SWCNT) to assess NP effects on zebrafish (Danio rerio) hatch. Exposure of 10 mg/L nAg and nCdSe delayed zebrafish hatch and 100 mg/L of nCdSe as well as 10 and 100 mg/L of uncoated nZnO completely inhibited hatch and the embryos died within the chorion. Both the morphology and the movement of the embryos were not affected, and it was determined that the main mechanism of hatch inhibition by NPs is likely through the interaction of NPs with the zebrafish hatching enzyme. Furthermore, it was concluded that the observed effects arose from the NPs themselves and not their dissolved metal components.

  8. High temperatures and absence of light affect the hatching of resting eggs of Daphnia in the tropics.

    PubMed

    Paes, Thécia A S V; Rietzler, Arnola C; Pujoni, Diego G F; Maia-Barbosa, Paulina M

    2016-03-01

    Temperature and light are acknowledged as important factors for hatching of resting eggs. The knowledge of how they affect hatching rates of this type of egg is important for the comprehension of the consequences of warming waters in recolonization of aquatic ecosystems dependent on dormant populations. This study aimed at comparing the influence of different temperature and light conditions on hatching rates of Daphnia ambigua andDaphnia laevis resting eggs from tropical environments. The ephippia were collected in the sediment of three aquatic ecosystems, in southeastern Brazil. For each lake, the resting eggs were exposed to temperatures of 20, 24, 28 and 32 °C, under light (12 h photoperiod) and dark conditions. The results showed that the absence of light and high temperatures have a negative influence on the hatching rates. Statistical differences for hatching rates were also found when comparing the studied ecosystems (ranging from 0.6 to 31%), indicating the importance of local environmental factors for diapause and maintenance of active populations.

  9. Spawning Characteristics and Artificial Hatching of Female Mottled Skate, Beringraja pulchra in the West Coast of Korea.

    PubMed

    Kang, Hee-Woong; Jo, Yeong-Rok; Kang, Duk-Yong; Jeong, Gyeong-Suk; Jo, Hyun-Su

    2013-09-01

    The gonadsomatic index (GSI) of mottled skate was the highest in April, GSI and HSI showed a reverse phase for its reproductive cycle. The fish had one pair of egg capsules, having 1 to 7 fertilized eggs, and spawned all the year round. When surveying the reproductive characteristics of females over 63 ㎝ in disc width, we found the spawning peak was between April to June, and the appearance ratio of egg capsules was the highest in May (32.1%). The eggs were hatched at 8°C, 13°C, 18°C, water temperature (12.8 to 24.2°C), and the best hatching temperature was 18°C. The number of fish hatched was 4 to 5 fish/egg capsules, and the hatching rate was 100%. The sex ratios of hatching larvae were 45.5% female and 54.5% male. Therefore this study will provide fundamental data and information for artificial reproduction of the mottled skate.

  10. Spawning Characteristics and Artificial Hatching of Female Mottled Skate, Beringraja pulchra in the West Coast of Korea

    PubMed Central

    Kang, Hee-Woong; Jo, Yeong-Rok; Kang, Duk-Yong; Jeong, Gyeong-Suk; Jo, Hyun-Su

    2013-01-01

    The gonadsomatic index (GSI) of mottled skate was the highest in April, GSI and HSI showed a reverse phase for its reproductive cycle. The fish had one pair of egg capsules, having 1 to 7 fertilized eggs, and spawned all the year round. When surveying the reproductive characteristics of females over 63 ㎝ in disc width, we found the spawning peak was between April to June, and the appearance ratio of egg capsules was the highest in May (32.1%). The eggs were hatched at 8°C, 13°C, 18°C, water temperature (12.8 to 24.2°C), and the best hatching temperature was 18°C. The number of fish hatched was 4 to 5 fish/egg capsules, and the hatching rate was 100%. The sex ratios of hatching larvae were 45.5% female and 54.5% male. Therefore this study will provide fundamental data and information for artificial reproduction of the mottled skate. PMID:25949140

  11. Can-out hatch assembly and positioning system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Basnar, P.J.; Frank, R.C.; Hoh, J.C.

    1985-07-03

    A can-out hatch assembly is adapted to engage in a sealed manner the upper end of a covered sealed container around an aperture in a sealed chamber and to remove the cover from the container permitting a contaminant to be transferred between the container and the chamber while isolating internal portions of the container and chamber from the surrounding environment. A swing bracket is coupled at a first end thereof to the inner, lower wall of the sealed container adjacent to the aperture therein. To a second end of the swing bracket is mounted a hatch cover which may bemore » positioned in sealed engagement about the chamber's aperture by rotating the hatch cover in a first direction when the swing bracket is in the full down position. Rotation of the hatch cover in a second direction release it from sealed engagement with the chamber's aperture. A lid support rod also coupled to the second end of the swing bracket and inserted through an aperture in the center of the hatch cover may be rotated for theadably engaging the container's cover whereupon the cover may be removed from the container and the hatch cover displaced from the aperture by pivoting displacement of the swing bracket. The contaminant may then be either removed from the container and placed within the sealed chamber, or vice versa, followed by positioning of the cover upon the container and the hatch cover over the aperture in a sealed manner.« less

  12. Fuel transfer tube quick opening hatch

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Meuschke, R. E.; Sherwood, D. G.; Silverblatt, B. L.

    1985-05-28

    A quick opening hatch for use on a transfer tube of a nuclear reactor plant that is adapted to replace the conventional hatch on the transfer tube. A locking ring is provided with a plurality of screw openings that is adapted for connection to the transfer tube, and a hatch cover fitably received within the locking ring for closing-off the transfer tube. To lock the cover to the ring, latches are movably connected with the cover for locking engagement with the locking ring, and a sprocket with a plurality of crank arms is movably connected with the cover and themore » latches for movement thereof into locking engagement with a latch housing on the locking ring for locking the cover to the ring and out of engagement with the latch housing for releasing the cover from the locking ring so as to permit removal of the hatch cover from the locking ring to provide access to the transfer tube. A davit assembly is provided which is connected with the transfer tube and the hatch cover to move the cover away and to provide guidance for closing-off the transfer tube. The locking ring and hatch cover also include cooperating keys and keyways for alignment when closing the transfer tube. The cover is provided with sealing rings and the latch housing and latches include cooperating cam surfaces to provide a tight locking engagement by compressing the sealing rings between the transfer tube and the hatch cover.« less

  13. Can-out hatch assembly and positioning system

    DOEpatents

    Basnar, P.J.; Frank, R.C.; Hoh, J.C.

    1985-07-03

    A can-out hatch assembly is adapted to engage in a sealed manner the upper end of a covered sealed container around an aperture in a sealed chamber and to remove the cover from the container permitting a contaminant to be transferred between the container and the chamber while isolating internal portions of the container and chamber from the surrounding environment. A swing bracket is coupled at a first end thereof to the inner, lower wall of the sealed container adjacent to the aperture therein. To a second end of the swing bracket is mounted a hatch cover which may be positioned in sealed engagement about the chamber's aperture by rotating the hatch cover in a first direction when the swing bracket is in the full down position. Rotation of the hatch cover in a second direction release it from sealed engagement with the chamber's aperture. A lid support rod also coupled to the second end of the swing bracket and inserted through an aperture in the center of the hatch cover may be rotated for theadably engaging the container's cover whereupon the cover may be removed from the container and the hatch cover displaced from the aperture by pivoting displacement of the swing bracket. The contaminant may then be either removed from the container and placed within the sealed chamber, or vice versa, followed by positioning of the cover upon the container and the hatch cover over the aperture in a sealed manner.

  14. Can-out hatch assembly and positioning system

    DOEpatents

    Basnar, Paul J.; Frank, Robert C.; Hoh, Joseph C.

    1986-01-01

    A can-out hatch assembly is adapted to engage in a sealed manner the upper end of a covered sealed container around an aperture in a sealed chamber and to remove the cover from the container permitting a contaminant to be transferred between the container and the chamber while isolating internal portions of the container and chamber from the surrounding environment. A swing bracket is coupled at a first end thereof to the inner, lower wall of the sealed container adjacent to the aperture therein. To a second end of the swing bracket is mounted a hatch cover which may be positioned in sealed engagement about the chamber's aperture by rotating the hatch cover in a first direction when the swing bracket is in the full down position. Rotation of the hatch cover in a second direction releases it from sealed engagement with the chamber's aperture. A lid support rod also coupled to the second end of the swing bracket and inserted through an aperture in the center of the hatch cover may be rotated for threadably engaging the container's cover whereupon the cover may be removed from the container and the hatch cover displaced from the aperture by pivoting displacement of the swing bracket. The contaminant may then be either removed from the container and placed within the sealed chamber, or vice versa, followed by positioning of the cover upon the container and the hatch cover over the aperture in a sealed manner.

  15. Can-out hatch assembly and positioning system

    DOEpatents

    Basnar, Paul J.; Frank, Robert C.; Hoh, Joseph C.

    1986-01-07

    A can-out hatch assembly is adapted to engage in a sealed manner the upper end of a covered sealed container around an aperture in a sealed chamber and to remove the cover from the container permitting a contaminant to be transferred between the container and the chamber while isolating internal portions of the container and chamber from the surrounding environment. A swing bracket is coupled at a first end thereof to the inner, lower wall of the sealed container adjacent to the aperture therein. To a second end of the swing bracket is mounted a hatch cover which may be positioned in sealed engagement about the chamber's aperture by rotating the hatch cover in a first direction when the swing bracket is in the full down position. Rotation of the hatch cover in a second direction releases it from sealed engagement with the chamber's aperture. A lid support rod also coupled to the second end of the swing bracket and inserted through an aperture in the center of the hatch cover may be rotated for threadably engaging the container's cover whereupon the cover may be removed from the container and the hatch cover displaced from the aperture by pivoting displacement of the swing bracket. The contaminant may then be either removed from the container and placed within the sealed chamber, or vice versa, followed by positioning of the cover upon the container and the hatch cover over the aperture in a sealed manner.

  16. Effects of Temperature and Root Leachates on Embryogenic Development and Hatching of Meloidogyne chitwoodi and M. hapla

    PubMed Central

    Inserra, R. N.; Griffin, G. D.; Sisson, D. V.

    1983-01-01

    At 20 C the duration of the embryogenic development of Meloiclogyne chitwoodi and M. hapla was about 20 days. At 10 C the embryogenic development was 82-84 days for M. chitwoodi and 95-97 days for M. hapla. The effect of distilled water and root leachates of potato cv. Russet Burbank, tomato cv. Columbian, and wheat cv. Hyslop on the hatching of eggs of the two root-knot nematode species was investigated at 4, 7, 10, 15, 20, and 25 C (± 1 C). Cumulative egg taatch was no greater in root leachates titan in distilled water, but temperature did significantly affect egg hatch (P = 0.05). Less than 1% of the eggs of both nematode species hatched at 4 C. The percent cumulative hatch at 10 C was significantly less (P = 0.05) than at higher temperatures for both nematodes and significantly more (P = 0.05) M. chitwoodi eggs hatched than did M. hapla eggs. At 15 G the percent cumulative hatch of both species was significantly lower (P = 0.05) than that at 20 and 25 C. The percent cumulative egg hatch of two species did not differ at 25 C, but was higher (P = 0.05) at 25 C than at 20 C. At 7 C the emergence of M. chitwoodi juveniles was about seven times (P = 0.01) greater than that of M. hapla in distilled water. PMID:19295777

  17. Aspects of hatching success and chick survival in Gull-billed Terns in coastal Virginia

    USGS Publications Warehouse

    Eyler, T.B.; Erwin, R.M.; Stotts, D.B.; Hatfield, J.S.

    1999-01-01

    Because of a long-term population decline in Gull-billed Terns (Sterna nilotica) nesting along the coast of Virginia, we began a three year study in 1994 to monitor hatching success and survival of Gull-billed Tern chicks at several Virginia colony sites. Colonies were located on either small, storm-deposited shellpiles along marsh fringes or large, sandshell overwash fans of barrier islands. Nests were monitored one to three times a week for hatching success, and enclosures were installed around selected nests to monitor chick survival from hatching to about two weeks of age. Hatching success was lower in marsh colonies than island colonies, and was lower in 1995 than in 1994 and 1996, primarily because of flooding. The average brood size of nests where at least one chick hatched was 1.99 chicks. Survival rates of chicks to 14 days depended on hatch order and year but not brood size (one vs. two or more) or time of season. A-chicks had higher survival rates than B-chicks and third-hatched C-chicks (0.661 compared to 0.442 and 0.357, respectively). The year effect was significant only for A-chicks, with lower survival in 1994 (0.50) than in 1995 (0.765) or 1996 (0.758). Overall, productivity was low (0.53 chick per nest) compared to estimates for colonies in Denmark, and was attributable to nest flooding by spring and storm-driven high tides and chick predation, presumably mostly by Great Horned Owls (Bubo virginianus).

  18. 37 CFR 1.84 - Standards for drawings.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... sheet. However, the relationship between the different parts must be clear and unambiguous. (3... hatching of juxtaposed different elements must be angled in a different way. In the case of large areas... hatched. Different types of hatching should have different conventional meanings as regards the nature of...

  19. 37 CFR 1.84 - Standards for drawings.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... sheet. However, the relationship between the different parts must be clear and unambiguous. (3... hatching of juxtaposed different elements must be angled in a different way. In the case of large areas... hatched. Different types of hatching should have different conventional meanings as regards the nature of...

  20. 46 CFR 108.114 - Appliances for watertight and weathertight integrity.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... watertight doors, hatches, scuttles, bolted manhole covers, or other watertight closures for openings in... and hatches, closures for air pipes, ventilators, ventilation intakes and outlets, and closures for... meet the following: (1) Each door, hatch, and scuttle must— (i) Be remotely controlled from a normally...

  1. 9 CFR 147.23 - Hatchery sanitation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... rooms are provided for each of the four operations: Egg receiving, incubation and hatching, chick/poult processing, and egg tray and hatching basket washing. Traffic and airflow patterns in the hatchery should be... should be cleaned and sanitized after each use. (d) The hatching compartments of incubators, including...

  2. 9 CFR 147.23 - Hatchery sanitation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... rooms are provided for each of the four operations: Egg receiving, incubation and hatching, chick/poult processing, and egg tray and hatching basket washing. Traffic and airflow patterns in the hatchery should be... should be cleaned and sanitized after each use. (d) The hatching compartments of incubators, including...

  3. 46 CFR 108.114 - Appliances for watertight and weathertight integrity.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... watertight doors, hatches, scuttles, bolted manhole covers, or other watertight closures for openings in... and hatches, closures for air pipes, ventilators, ventilation intakes and outlets, and closures for... meet the following: (1) Each door, hatch, and scuttle must— (i) Be remotely controlled from a normally...

  4. 46 CFR 108.114 - Appliances for watertight and weathertight integrity.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... watertight doors, hatches, scuttles, bolted manhole covers, or other watertight closures for openings in... and hatches, closures for air pipes, ventilators, ventilation intakes and outlets, and closures for... meet the following: (1) Each door, hatch, and scuttle must— (i) Be remotely controlled from a normally...

  5. 2. VIEW OF THE HATCH ADIT (FEATURE B28), FACING NORTH. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF THE HATCH ADIT (FEATURE B-28), FACING NORTH. ADIT ROAD IS VISIBLE IN THE FOREGROUND AND OFFICE (FEATURE B-1) IN THE BACKGROUND. - Nevada Lucky Tiger Mill & Mine, Hatch Adit, East slope of Buckskin Mountain, Paradise Valley, Humboldt County, NV

  6. The Hatch Act and Inflation.

    ERIC Educational Resources Information Center

    Baird, Charles W.

    1980-01-01

    Repeal of the Hatch Act would worsen inflation and make effective controls over government much more difficult to achieve. Repeal of the Hatch Act would serve the interests of no one except those who seek to gain increasing political power at the expense of the general public. (Author/IRT)

  7. 9 CFR 147.23 - Hatchery sanitation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... rooms are provided for each of the four operations: Egg receiving, incubation and hatching, chick/poult processing, and egg tray and hatching basket washing. Traffic and airflow patterns in the hatchery should be... should be cleaned and sanitized after each use. (d) The hatching compartments of incubators, including...

  8. 46 CFR 108.114 - Appliances for watertight and weathertight integrity.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... watertight doors, hatches, scuttles, bolted manhole covers, or other watertight closures for openings in... and hatches, closures for air pipes, ventilators, ventilation intakes and outlets, and closures for... meet the following: (1) Each door, hatch, and scuttle must— (i) Be remotely controlled from a normally...

  9. 46 CFR 108.114 - Appliances for watertight and weathertight integrity.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... watertight doors, hatches, scuttles, bolted manhole covers, or other watertight closures for openings in... and hatches, closures for air pipes, ventilators, ventilation intakes and outlets, and closures for... meet the following: (1) Each door, hatch, and scuttle must— (i) Be remotely controlled from a normally...

  10. 29 CFR 1918.35 - Open hatches.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 7 2014-07-01 2014-07-01 false Open hatches. 1918.35 Section 1918.35 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.35 Open hatches. Open weather deck...

  11. 29 CFR 1918.35 - Open hatches.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Open hatches. 1918.35 Section 1918.35 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.35 Open hatches. Open weather deck...

  12. 29 CFR 1918.35 - Open hatches.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 7 2011-07-01 2011-07-01 false Open hatches. 1918.35 Section 1918.35 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.35 Open hatches. Open weather deck...

  13. 29 CFR 1918.35 - Open hatches.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 7 2012-07-01 2012-07-01 false Open hatches. 1918.35 Section 1918.35 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.35 Open hatches. Open weather deck...

  14. 29 CFR 1918.35 - Open hatches.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 7 2013-07-01 2013-07-01 false Open hatches. 1918.35 Section 1918.35 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Working Surfaces § 1918.35 Open hatches. Open weather deck...

  15. 9 CFR 91.29 - Hatches.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... animal carcasses. Such hatches shall be watertight. [42 FR 28990, June 7, 1977. Redesignated at 45 FR... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Hatches. 91.29 Section 91.29 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION...

  16. 76 FR 1400 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ... number. Animal and Plant Health Inspection Service Title: Certificate for Poultry and Hatching Eggs for... poultry. The export of agricultural commodities, including poultry and hatching eggs is a major business... import health requirements of other countries for poultry and hatching eggs exported from the U.S. Most...

  17. 9 CFR 93.105 - Inspection at the port of entry.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) All commercial birds, zoological birds, and research birds, including hatching eggs of ratites, but... an international airport, serviced by Customs, as well as, for Canadian-origin hatching eggs of ratites, ports listed in § 93.107 (c). However, hatching eggs of ratites may be shipped, in bond, from the...

  18. 9 CFR 93.105 - Inspection at the port of entry.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) All commercial birds, zoological birds, and research birds, including hatching eggs of ratites, but... an international airport, serviced by Customs, as well as, for Canadian-origin hatching eggs of ratites, ports listed in § 93.107 (c). However, hatching eggs of ratites may be shipped, in bond, from the...

  19. Gidzenko at ISS hatch

    NASA Image and Video Library

    2001-02-10

    ISS01-E-5325 (10 February 2001) --- Cosmonaut Yuri P. Gidzenko, Expedition One Soyuz commander, stands near the hatch leading from the Unity node into the newly attached Destiny laboratory aboard the International Space Station (ISS). The picture was recorded with a digital still camera on the day the hatch was initially opened.

  20. 9 CFR 93.209 - Quarantine requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., other than eggs for hatching, imported, except as provided in § 93.216 of this part, shall be... from exposure to such diseases. (b) Poultry eggs for hatching imported, except from regions designated... of arrival at the port of entry until hatched and the poultry from such eggs shall remain quarantined...

  1. 9 CFR 145.6 - Specific provisions for participating hatcheries.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., ceilings, floors, doors, fans, vents, and ducts should be cleaned and disinfected after each hatch. Hatcher rooms should be cleaned and disinfected after each hatch and should not be used for storage. Plenums should be cleaned after each hatch. Cleaning and disinfection procedures should be as outlined in § 147...

  2. 9 CFR 93.105 - Inspection at the port of entry.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) All commercial birds, zoological birds, and research birds, including hatching eggs of ratites, but... an international airport, serviced by Customs, as well as, for Canadian-origin hatching eggs of ratites, ports listed in § 93.107 (c). However, hatching eggs of ratites may be shipped, in bond, from the...

  3. Protection of Students' Privacy Rights: The Hatch Amendment.

    ERIC Educational Resources Information Center

    Mesibov, Laurie

    1985-01-01

    Widely divergent interpretations are being made of the implementing regulations to the Hatch Amendment, which gives parents the right to inspect instructional materials and to limit their children's participation in some federally supported school programs and activities. This article briefly explains the Hatch Amendment and suggests a source of…

  4. 49 CFR 176.182 - Conditions for handling on board ship.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Class 1 (explosive) materials. During electrical storms, cargo operations must be halted and all hatches...) All hatches and cargo ports opening into a compartment in which Class 1 (explosive) materials are stowed must be kept closed except during loading and unloading of the compartment. After loading, hatches...

  5. 49 CFR 176.182 - Conditions for handling on board ship.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Class 1 (explosive) materials. During electrical storms, cargo operations must be halted and all hatches...) All hatches and cargo ports opening into a compartment in which Class 1 (explosive) materials are stowed must be kept closed except during loading and unloading of the compartment. After loading, hatches...

  6. 77 FR 3187 - Airworthiness Directives; the Boeing Company Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-23

    ... of the hatch opening of the overwing emergency exit. This proposed AD would require repetitive... cracking on the lower main sill inner chord of the hatch opening of the overwing emergency exit, which could result in reduced structural integrity of the hatch opening of the overwing emergency exit and...

  7. 49 CFR 176.182 - Conditions for handling on board ship.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Class 1 (explosive) materials. During electrical storms, cargo operations must be halted and all hatches...) All hatches and cargo ports opening into a compartment in which Class 1 (explosive) materials are stowed must be kept closed except during loading and unloading of the compartment. After loading, hatches...

  8. 9 CFR 145.6 - Specific provisions for participating hatcheries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., ceilings, floors, doors, fans, vents, and ducts should be cleaned and disinfected after each hatch. Hatcher rooms should be cleaned and disinfected after each hatch and should not be used for storage. Plenums should be cleaned after each hatch. Cleaning and disinfection procedures should be as outlined in § 147...

  9. 9 CFR 145.6 - Specific provisions for participating hatcheries.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., ceilings, floors, doors, fans, vents, and ducts should be cleaned and disinfected after each hatch. Hatcher rooms should be cleaned and disinfected after each hatch and should not be used for storage. Plenums should be cleaned after each hatch. Cleaning and disinfection procedures should be as outlined in § 147...

  10. 9 CFR 93.209 - Quarantine requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., other than eggs for hatching, imported, except as provided in § 93.216 of this part, shall be... from exposure to such diseases. (b) Poultry eggs for hatching imported, except from regions designated..., shall be quarantined from time of arrival at the port of entry until hatched and the poultry from such...

  11. 9 CFR 93.209 - Quarantine requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., other than eggs for hatching, imported, except as provided in § 93.216 of this part, shall be... from exposure to such diseases. (b) Poultry eggs for hatching imported, except from regions designated... influenza, shall be quarantined from time of arrival at the port of entry until hatched and the poultry from...

  12. 9 CFR 93.209 - Quarantine requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., other than eggs for hatching, imported, except as provided in § 93.216 of this part, shall be... from exposure to such diseases. (b) Poultry eggs for hatching imported, except from regions designated... of arrival at the port of entry until hatched and the poultry from such eggs shall remain quarantined...

  13. 49 CFR 176.182 - Conditions for handling on board ship.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Class 1 (explosive) materials. During electrical storms, cargo operations must be halted and all hatches...) All hatches and cargo ports opening into a compartment in which Class 1 (explosive) materials are stowed must be kept closed except during loading and unloading of the compartment. After loading, hatches...

  14. 9 CFR 93.209 - Quarantine requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., other than eggs for hatching, imported, except as provided in § 93.216 of this part, shall be... from exposure to such diseases. (b) Poultry eggs for hatching imported, except from regions designated... influenza, shall be quarantined from time of arrival at the port of entry until hatched and the poultry from...

  15. 46 CFR 72.05-35 - Hatch covers and shifting boards.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-35 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS CONSTRUCTION AND ARRANGEMENT Structural Fire Protection § 72.05-35 Hatch covers and shifting boards. (a) Wood hatch... deck construction noted in tables 72.05-10 (f) and (g). (b) Tonnage openings in “A” Class bulkheads...

  16. Enterobacteriaceae and salmonella recovered from non-sanitized and sanitized broiler hatching eggs

    USDA-ARS?s Scientific Manuscript database

    Inhibiting Salmonella contamination of hatching eggs could reduce the chance of broiler chicks becoming colonized during incubation and hatching. An experiment was conducted to determine the efficacy of a sanitizer (1,200 ppm quaternary ammonium- biguanide compound) applied as foam or spray in redu...

  17. 76 FR 31602 - Combined Notice of Filings #2

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    .... Applicants: Hatch Solar Energy Center I, LLC. Description: Hatch Solar Energy Center I, LLC submits tariff filing per 35.12: Hatch Solar Energy Center I, LLC MBR Application to be effective 5/26/2011. Filed Date... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Combined Notice of Filings 2 Take notice...

  18. The adaptive significance of hatching synchrony of waterfowl eggs

    USGS Publications Warehouse

    Flint, Paul L.; Lindberg, Mark S.; MacCluskie, Margaret C.; Sedinger, James S.

    1994-01-01

    We estimated the amount of incubation time that first laid Black Brent eggs received before completion of the clutch. First laid eggs received up to 48 hours of incubation before the last egg was laid in Brent clutches. Waterfowl clutches usually hatch within a period of 24 hours, suggesting that some mechanism reduces developmental asynchrony during incubation. The combination of incubation during laying and hatch synchronization mechanisms should be adaptive because these traits reduce nest exposure, maintain egg viability, and result in an earlier hatch date, all of which increase fitness in waterfowl.

  19. The influence of non-ionic radiation on the chicken hatching.

    PubMed

    Veterány, Ladislav; Toman, Robert; Jedlicka, Jaroslav

    2002-11-01

    The study considers the influence of non-ionic radiation (white and monochromatic light) on the hatching of the Hampshire breed chickens. The chicken embryos were most sensitive to the white light (El), reaching the hatching time of 503.63 +/- 3.17 h, the hatchability of 95.12 +/- 3.72% and an average weight of incubated chickens 46.83 +/- 2.82 g. Of the monochromatic lights, the chicken embryos were most sensitive to yellow and green lights (E5, E4) with the hatching time of 505.22 +/- 4.03 and 507.14 +/- 3.95 h, respectively, the hatchability of 94.89 +/- 3.02 and 94.47 +/- 2.93%, respectively and the average weight of incubated chickens 45.72 +/- 1.93 and 45.05 +/- 2.66 g, respectively. The least reaction of chicken was observed with violet light (E2) with the hatching time of 510.04+/- 1.97 h, hatchability of 90.81 +/- 4.05% and the average weight of incubated chickens 42.02 +/- 3.72 g. The effect of violet light brings the same results as we observed in the case of hatching in darkness (control group C), when the hatching time was 510.41 +/- 2.82 h, hatchability 90.42 +/- 3.35% and average weight of incubated chickens 41.98 +/- 3.05 g.

  20. A new predictor of atrial fibrillation after coronary artery bypass graft surgery: HATCH score.

    PubMed

    Selvi, Mithat; Gungor, Hasan; Zencir, Cemil; Gulasti, Sevil; Eryilmaz, Ufuk; Akgullu, Cagdas; Durmaz, Selim

    2018-03-01

    The aim of this study was to investigate the association between HATCH score and atrial fibrillation (AF) after coronary artery bypass graft (CABG) surgery. 369 patients (103 patients with AF and 266 patients without AF) undergoing isolated CABG surgery were analyzed. Complete medical records were retrospectively collected to investigate HATCH score. The median age of patients with AF was significantly higher than the median age of non-AF group (60.8±10.0 years vs 67.8±9.5 years, P<0.001). HATCH score was significantly higher in patients who developed AF after CABG surgery than the non-AF group (P=0.017). Multivariate logistic regression analysis showed that HATCH score (OR 1.334; 95% CI 1.022 to 1.741, P=0.034) was an independent predictor of AF after CABG surgery. Receiver operating characteristic curve analysis showed that the cut-off point of HATCH score related to predict AF was >1 (two or more), with a sensitivity of 42% and specificity of 70%. Patients with elevated preoperative HATCH score may have higher risk for AF after CABG surgery. © American Federation for Medical Research (unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  1. Hatching the Cleidoic Egg: The Role of Thyroid Hormones

    PubMed Central

    De Groef, Bert; Grommen, Sylvia V.H.; Darras, Veerle M.

    2013-01-01

    A major life stage transition in birds and other oviparous sauropsids is the hatching of the cleidoic egg. Not unlike amphibian metamorphosis, hatching in these species can be regarded as a transition from a relatively well-protected “aqueous” environment to a more hazardous and terrestrial life outside the egg, a transition in which thyroid hormones (THs) (often in concert with glucocorticoids) play an important role. In precocial birds such as the chicken, the perihatch period is characterized by peak values of THs. THs are implicated in the control of muscle development, lung maturation and the switch from chorioallantoic to pulmonary respiration, yolk sac retraction, gut development and induction of hepatic genes to accommodate the change in dietary energy source, initiation of thermoregulation, and the final stages of brain maturation as well as early post-hatch imprinting behavior. There is evidence that, at least for some of these processes, THs may have similar roles in non-avian sauropsids. In altricial birds such as passerines on the other hand, THs do not rise significantly until well after hatching and peak values coincide with the development of endothermy. It is not known how hatching-associated processes are regulated by hormones in these animals or how this developmental mode evolved from TH-dependent precocial hatching. PMID:23755041

  2. Infanticide and within-clutch competition select for reproductive synchrony in a cooperative bird.

    PubMed

    Riehl, Christina

    2016-08-01

    Reproduction among members of social animal groups is often highly synchronized, but neither the selective advantages nor the proximate causes of synchrony are fully understood. Here I investigate the evolution of hatching synchrony in the Greater Ani (Crotophaga major), a communally nesting bird in which several unrelated females contribute eggs to a large, shared clutch. Hatching synchrony is variable, ranging from complete synchrony to moderate asynchrony, and is determined by the onset of incubation of the communal clutch. Data from a 10-year field study indicate that individual reproductive success is highest in synchronous groups, and that nestlings that hatch in the middle of the hatching sequence are most likely to survive. Nestling mortality is high in asynchronous clutches because early-hatching nestlings are more likely to be killed by adult group members, whereas late-hatching nestlings are more likely to starve due competition with their older nest-mates. Therefore, the timing of hatching appears to be under stabilizing selection from infanticide and resource competition acting in concert. These results provide empirical support for models predicting that synchrony may evolve as an adaptive counter-strategy to infanticide, and they highlight the importance of competition in shaping the timing of reproduction in social groups. © 2016 The Author(s). Evolution © 2016 The Society for the Study of Evolution.

  3. Multi-response optimization of Artemia hatching process using split-split-plot design based response surface methodology

    PubMed Central

    Arun, V. V.; Saharan, Neelam; Ramasubramanian, V.; Babitha Rani, A. M.; Salin, K. R.; Sontakke, Ravindra; Haridas, Harsha; Pazhayamadom, Deepak George

    2017-01-01

    A novel method, BBD-SSPD is proposed by the combination of Box-Behnken Design (BBD) and Split-Split Plot Design (SSPD) which would ensure minimum number of experimental runs, leading to economical utilization in multi- factorial experiments. The brine shrimp Artemia was tested to study the combined effects of photoperiod, temperature and salinity, each with three levels, on the hatching percentage and hatching time of their cysts. The BBD was employed to select 13 treatment combinations out of the 27 possible combinations that were grouped in an SSPD arrangement. Multiple responses were optimized simultaneously using Derringer’s desirability function. Photoperiod and temperature as well as temperature-salinity interaction were found to significantly affect the hatching percentage of Artemia, while the hatching time was significantly influenced by photoperiod and temperature, and their interaction. The optimum conditions were 23 h photoperiod, 29 °C temperature and 28 ppt salinity resulting in 96.8% hatching in 18.94 h. In order to verify the results obtained from BBD-SSPD experiment, the experiment was repeated preserving the same set up. Results of verification experiment were found to be similar to experiment originally conducted. It is expected that this method would be suitable to optimize the hatching process of animal eggs. PMID:28091611

  4. Variations in early life history traits of Japanese anchovy Engraulis japonicus in the Yangtze River Estuary.

    PubMed

    Liu, Chunlong; Xian, Weiwei; Liu, Shude; Chen, Yifeng

    2018-01-01

    Resources of Japanese anchovy ( Engraulis japonicus Temminck & Schlegel, 1846) are undergoing dramatic recessions in China as the consequence of intensifying anthropogenic activities. Elucidating the influences of local-scale environmental factors on early life history traits is of great importance to design strategies conserving and restoring the declining anchovy resources. In this research, we studied hatching date and early growth of anchovy in the Yangtze River Estuary (YRE) using information obtained from otolith microstructure. Onset of hatching season and growth rates of anchovy was compared to populations in Japan and Taiwan. In YRE, the hatching date of anchovy ranged from February 26th to April 6th and mean growth rate ranged from 0.27 to 0.77 mm/d. Anchovies hatching later had higher growth rates than individuals hatching earlier before the 25th day. Among populations, hatching onsets of anchovy from the higher latitude were later than populations in the lower latitude, and growth rates of anchovy in YRE were much lower than populations in Japan and Taiwan. Variations in hatching onsets and early growth patterns of anchovy thus provide important knowledge on understanding the adaptation of anchovy in YRE and designing management strategies on conserving China's anchovy resources.

  5. Stable isotopic investigations of early development in extant and fossil chambered cephalopods I. Oxygen isotopic composition of eggwater and carbon isotopic composition of siphuncle organic matter in Nautilus

    NASA Astrophysics Data System (ADS)

    Crocker, Kimberley C.; DeNiro, Michael J.; Ward, Peter D.

    1985-12-01

    Eggwaters from the chambered cephalopod Nautilus are depleted in both 18O and deuterium relative to ambient seawater. Eggwaters from six other species, including the related chambered cephalopod Sepia, do not show such depletion. These observations indicate that the previously observed step towards more positive δ 18O values in calcium carbonate laid down after Nautilus hatches, relative to carbonate precipitated prior to hatching, can be explained by equilibration of the carbonate with water in the egg before hatching and with seawater after hatching. The presence of an oxygen isotope difference between eggwater and seawater for Nautilus and its absence for Sepia suggest that hatching will be recorded in the δ 18O values of shell carbonates for some but not all extinct and extant chambered cephalopods. The δ 13C values of the organic fraction of the siphuncle in Nautilus do not show any consistent pattern with regard to the time of formation before or after hatching. This observation suggests that the minimum in δ 13C values previously observed for calcium carbonate precipitated after Nautilus hatches is not caused by a change in food sources once the animal becomes free-swimming, as has been suggested.

  6. Effect of in ovo feeding of dextrin-iodinated casein in broilers: II. Hatch window and growth performance.

    PubMed

    Abousaad, S; Lassiter, K; Piekarski, A; Chary, P; Striplin, K; Christensen, K; Bielke, L R; Hargis, B M; Dridi, S; Bottje, W G

    2017-05-01

    Studies were conducted using a commercial InovojectTM system to determine effects of in ovo feeding of dextrin and iodinated casein (IC) on hatch and posthatch growth in broilers. At ∼18.5 d embryonic development, eggs were treated with 0, 240, or 480 μg IC/mL in saline (Cont, IC240, and IC480) or dextrin (Dext, DextIC240 and DextIC480). The Dext solution consisted of 18% maltodextrin and 10% potato starch dextrin; saline was the vehicle used by the company for in ovo vaccination. The volume for all in ovo treatments was 50 μL/injection. Eggs in Experiment 1 were transferred to a commercial hatcher unit whereas eggs in Experiments 2 and 3 were transferred to a research hatcher unit to assess effects of treatments on timing of hatch. At hatch, chicks were randomly selected and placed in floor pens and grown to 6 wk. In Experiment 1, there were no differences in hatch weights, but broilers provided Dext IC240 in ovo were heavier (P < 0.05) at 6 wk compared to other treatments with the exception of the Dext IC240 group. In Experiment 2, hatch weights were heavier (P < 0.05) in chicks receiving IC240 and DexIC480 treatments compared to Controls. At 6 wk, broilers in all treatments were heavier (P < 0.05) than Cont with the exception of IC480. In Experiment 3, hatch was stimulated by IC240 (in saline), but was delayed by Dext IC240. Serum analysis of β-hydroxybutyrate (μM/mL), as an indicator of ketone accumulation from fat metabolism of chicks held in chick boxes for 24 h posthatch (to simulate delay in placement after hatch), indicated that chicks in the IC240 group (that hatched earlier) had higher blood ketones compared to chicks that received Dext or DextIC240 in ovo (that hatched later). We conclude dextrin and iodinated casein (240 μg/mL) provided in ovo (∼18.5 d of embryonic development) has the potential to improve chick quality and posthatch body weight by delaying or narrowing hatch window. © 2016 Poultry Science Association Inc.

  7. Laser-assisted zona pellucida thinning does not facilitate hatching and may disrupt the in vitro hatching process: a morphokinetic study in the mouse.

    PubMed

    Schimmel, Tim; Cohen, Jacques; Saunders, Helen; Alikani, Mina

    2014-12-01

    Does laser-assisted zona thinning of cleavage stage mouse embryos facilitate hatching in vitro? No, unlike laser zona opening, zona thinning does not facilitate embryo hatching. Artificial opening of the zona pellucida facilitates hatching of mouse and human embryos. Laser-assisted zona thinning has also been used for the purpose of assisted hatching of human embryos but it has not been properly investigated in an animal model; thinning methods have produced inconsistent clinical results. Time-lapse microscopy was used to study the hatching process in the mouse after zona opening and zona thinning; a control group of embryos was not zona-manipulated but exposed to the same laser energy. Eight-cell CB6F1/J mouse embryos were pooled and allocated to three groups (n = 56 per group): A control group of embryos that were exposed to a dose of laser energy focused outside the zona pellucida (zona intact); one experimental group of embryos in which the zona pellucida was opened by complete ablation using the same total number of pulses as the control group; a second experimental group of embryos in which the zona pellucida was thinned to establish a smooth lased area using the same number of pulses as used in the other two groups. The width of the zona opening was 25 μm and width of the thinned area was 35 μm. Development was monitored by time-lapse microscopy. Overall treatment differences for continuous variables were analyzed by analysis of variance and pairwise comparisons using the Student t-test allowing for unequal variances, while for categorical data, a standard chi-squared test was utilized for all pairwise comparisons. The frequency of complete hatching was 33.9% in the control group, 94.4% after zona opening, and 39.3% after zona thinning (overall group comparison, P < 0.0001). Overall, 60.7% of the zona-thinned embryos did not complete the hatching process and remained trapped within the zona; when they did hatch, they did not necessarily hatch from the zona-thinned area. Hatching in about one-third of the zona-intact embryos began with breaches at multiple sites by small groups of cells. Likewise, 53.6% of zona-thinned embryos had multiple breaches, always involving an area outside the thinned zone. Zona opening decreased multiple breaching and led to blastocyst escape an average of 14 h earlier than zona-thinned embryos and 5.5 h before control embryos (P = 0.0003). The experiments presented here were limited to in vitro experiments performed in the mouse. Whether human embryos would behave the same way under similar circumstances is unknown. We postulate that zona thinning is not beneficial in human embryos. The experiments demonstrate that zona thinning is not equivalent to zona opening for assisted hatching. The study provides reason for systematic reviews of assisted hatching trials to take the method of assisted hatching into consideration and not combine the results of zona thinning and zona opening procedures. Institutional funds were used for the study. No competing interests are declared. © The Author 2014. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

  8. Effect of nesting environment on incubation temperature and hatching success of Morelet's crocodile (Crocodylus moreletii) in an urban lake of Southeastern Mexico.

    PubMed

    López-Luna, Marco A; Hidalgo-Mihart, Mircea G; Aguirre-León, Gustavo; González-Ramón, Mariana Del C; Rangel-Mendoza, Judith A

    2015-01-01

    Incubation temperature is an important aspect in terms of biological performance among crocodiles, and several controlled experiments have demonstrated a significant relationship between incubation temperature, success in hatching and survival of hatchlings. However, a few studies have tested these relationships in the wild. The objective of this study was to determine the relationship of nest characteristics and environment (hatch year, nest basal area and height, clutch size, distance to shore line, and vegetation cover), to incubation temperature and hatching success among Morelet's crocodile (Crocodylus moreletii). The study was carried out during the nesting seasons of Morelet's crocodile, from 2007 to 2009 in the Laguna de Las Ilusiones, an urban lake located in Villahermosa, Tabasco, Mexico. We physically characterized 18 nests and inserted a temperature data logger in each nest chamber. At the end of the nesting season and prior to hatching, we recovered the crocodile eggs and data loggers and calculated hatching success, under laboratory conditions. We related the environmental variables of the nest with the mean and fluctuation (standard deviation) of nest temperature, using linear models. We also related the environmental variables affecting the nest, to mean nest temperature and fluctuation in incubation temperature and to hatching success, using linear models. Although we found differences in incubation temperature between nests, mean incubation temperature did not differ between years, but there were differences in nest thermal fluctuation between years. The mean incubation temperature for 11 nests (61.1%) was lower than the suggested Female-Male pivotal temperature (producing 50% of each sex) for this species, and all hatchlings obtained were males. There were no differences in clutch size between years, but hatching success varied. Our study indicates that hatching success depends on certain environmental variables and nest conditions to which the eggs are subjected, including season, nest size and clutch size. We also discuss the importance of the fluctuation of incubation temperature on hatching success and sex determination. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Development of the hypothalamus and pituitary in platypus (Ornithorhynchus anatinus) and short-beaked echidna (Tachyglossus aculeatus)

    PubMed Central

    Ashwell, Ken W S

    2012-01-01

    The living monotremes (platypus and echidnas) are distinguished by the development of their young in a leathery-shelled egg, a low and variable body temperature and a primitive teat-less mammary gland. Their young are hatched in an immature state and must deal with the external environment, with all its challenges of hypothermia and stress, as well as sourcing nutrients from the maternal mammary gland. The Hill and Hubrecht embryological collections have been used to follow the structural development of the monotreme hypothalamus and its connections with the pituitary gland both in the period leading up to hatching and during the lactational phase of development, and to relate this structural maturation to behavioural development. In the incubation phase, development of the hypothalamus proceeds from closure of the anterior neuropore to formation of the lateral hypothalamic zone and putative medial forebrain bundle. Some medial zone hypothalamic nuclei are emerging at the time of hatching, but these are poorly differentiated and periventricular zone nuclei do not appear until the first week of post-hatching life. Differentiation of the pituitary is also incomplete at hatching, epithelial cords do not develop in the pars anterior until the first week, and the hypothalamo-neurohypophyseal tract does not appear until the second week of post-hatching life. In many respects, the structure of the hypothalamus and pituitary of the newly hatched monotreme is similar to that seen in newborn marsupials, suggesting that both groups rely solely on lateral hypothalamic zone nuclei for whatever homeostatic mechanisms they are capable of at birth/hatching. PMID:22512474

  10. Production traits of artificially and naturally hatched geese in intensive and free-range systems: I. Growth traits.

    PubMed

    Boz, M A; Sarica, M; Yamak, U S

    2017-04-01

    1. This study investigated the effect of incubation type and production system on geese growth traits. 2. A total of 216 geese were either naturally (114) or artificially (102) hatched and reared in intensive or free-range production systems (4 replicates each) until 18 weeks of age. 3. Weights of naturally hatched goslings (NHG) were significantly higher than artificially hatched goslings (AHG) at 2 weeks (644 vs. 536 g); however, weights of AHG were significantly higher than NHG at both 6 weeks (3245 vs. 3010 g) and 18 weeks (5212 vs. 4353 g). 4. AHG had better feed conversion ratios (FCRs) than NHG (6.21 vs. 6.46 at 18 weeks). Feed consumption of naturally hatched geese was found higher in first 4 weeks when compared to artificially hatched geese and artificially hatched geese consumed more feed than naturally hatched geese after 8 weeks. 5. Production system had insignificant effects on feed consumption, FCRs, viability and mutilation rates. 6. Slipped wings were more frequent in NHG than AHG (8.32% vs. 1.68% at 6 weeks; 23.84% vs. 5.12% between 7 and 18 weeks) and in free-range production when compared to intensive production (17.88% vs. 11.08% over the course of the production period). 7. The study results indicate that both artificially and NHG can be reared in free-range production systems without any loss in performance and in deference to animal welfare.

  11. Effects of mercury on the embryos and larvae of the freshwater teleosts: Etheostoma caeruleum and E. spectabile

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sharp, J.R.

    1994-12-31

    A 24-h static renewal assay of five replicates of ten cleavage stage (8-1 6 cell) embryos each of the percid teleosts Etheostoma caeruleum (Ec) and E. spectabile (Es) were exposed to 0--100 ppb Hg++ (mercuric chloride) until all embryos had hatched or died. This assay was designed to determine concentration-specific and species-specific differences in embryonic mortality, teratogenesis, hatchability, viability of hatch, heart rate, and growth. In a separate assay embryos were exposed to lower mercury concentrations through a 30-d post hatch exposure to evaluate longer term effects on larval survival and growth. At 18 C, Etheostoma caeruleum and E. spectabilemore » have average incubation periods (time to hatch) and ova diameters of 12-d, 8-d; and 1.9mm, 1.2mm; respectively. Four median effective concentrations, as ppb Hg++, were estimated as a result of embryonic exposure: 96-LC50 (lethality), AB50 (abnormality), SH50 (successful hatch) and VH50 (viable hatch). The typical and predictable embryonic and larval terata were noted for both species. Cardiac pulsation rates (beats/minute) were significantly reduced at > 20 ppb for both species. Mean total length of first day hatch for Ec and Es was significantly shorter for embryo emerging from 5 ppb and 10 ppb, respectively. Post-hatch survival (after 30-d) for both species was significantly reduced at 5 ppb. Larval growth, after 30-d, was significantly less at 2.5 and 5 ppb for Ec and Es, respectively.« less

  12. 29 CFR 780.211 - Contract production of hatching eggs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Contract production of hatching eggs. 780.211 Section 780... eggs. It is common practice for hatcherymen to enter into arrangements with farmer poultry raisers for the production of hatching eggs which the hatchery agrees to buy. Ordinarily, the farmer furnishes the...

  13. 5 CFR 734.102 - Jurisdiction.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... prohibited by the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, prosecute alleged... the District of Columbia government. (5 U.S.C. 1212 and 1216. Advice concerning the Hatch Act Reform... whether a violation of the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, has...

  14. 5 CFR 734.102 - Jurisdiction.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... prohibited by the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, prosecute alleged... the District of Columbia government. (5 U.S.C. 1212 and 1216. Advice concerning the Hatch Act Reform... whether a violation of the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, has...

  15. Krikalev and Gidzenko at ISS hatch

    NASA Image and Video Library

    2001-02-10

    ISS-01-E-5324 (10 February 2001) --- Cosmonauts Sergei K. Krikalev (left), Expedition One flight engineer, and Yuri P. Gidzenko, Soyuz commander, are pictured at the hatch that leads from the Unity node into the newly attached Destiny laboratory. The picture was recorded with a digital still camera on the day the hatch was initially opened.

  16. 5 CFR 734.102 - Jurisdiction.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... prohibited by the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, prosecute alleged... the District of Columbia government. (5 U.S.C. 1212 and 1216. Advice concerning the Hatch Act Reform... whether a violation of the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, has...

  17. The Hatch Amendment Regulations: Lessons for Social Studies Educators.

    ERIC Educational Resources Information Center

    Greene, Bert I.; Pasch, Marvin.

    1986-01-01

    Briefly reviews the history of the Hatch Amendment Regulations. Offers five lessons that can be learned from its passage. Concludes that we must develop better rapport with parents, including them as committee advisory members and making sure that we are not violating the substance or spirit of the Hatch Amendment. (JDH)

  18. View of Yurchikhin preparing for the STS-117 Hatch Opening during Expedition 15

    NASA Image and Video Library

    2007-06-10

    ISS015-E-11769 (10 June 2007) --- Cosmonaut Fyodor N. Yurchikhin, Expedition 15 commander representing Russia's Federal Space Agency, photographed near a hatch door in the Destiny laboratory of the International Space Station. Shortly after this image was taken, the hatches were opened between the station and Space Shuttle Atlantis.

  19. 75 FR 34759 - Emergency Exemption: Issuance of Permit for Endangered Species

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-18

    ... Foundation, Loxahatchee, Florida, to import one captive- hatched imperial parrot (Amazona imperialis) for the... requested a permit to import the above-mentioned imperial parrot chick captive-hatched on May 6, 2010, at... species hatched in captivity anywhere in the world and was being parent-reared at the aviary until it was...

  20. Protandry of western corn rootworm (Coleoptera: Chrysomelidae) beetle emergence partially due to earlier egg hatch of males

    USDA-ARS?s Scientific Manuscript database

    The western corn rootworm, Diabrotica virgifera virgifera LeConte, exhibits protandry. The contribution of pre-hatch development to protandry in western corn rootworm was previously investigated with a small set of data from one population. To verify the contribution of pre-hatch development to prot...

  1. 5 CFR 734.102 - Jurisdiction.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... prohibited by the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, prosecute alleged... the District of Columbia government. (5 U.S.C. 1212 and 1216. Advice concerning the Hatch Act Reform... whether a violation of the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, has...

  2. 5 CFR 734.102 - Jurisdiction.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... prohibited by the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, prosecute alleged... the District of Columbia government. (5 U.S.C. 1212 and 1216. Advice concerning the Hatch Act Reform... whether a violation of the Hatch Act Reform Amendments of 1993, as implemented by 5 CFR part 734, has...

  3. 77 FR 36328 - Agency Information Collection Activities; Request for Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ... interpretation and enforcement of Hatch Act provisions on political activity in chapters 15 and 73 of title 5 of... 3255-0002; (3) Form OSC-13 (Complaint of Possible Prohibited Political Activity (Violation of the Hatch..., (2) provide a safe, secure channel for whistleblower disclosures, and (3) enforce the Hatch Act. The...

  4. 49 CFR 238.123 - Emergency roof access.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... inches laterally. (b) Means of access. Emergency roof access shall be provided by means of a hatch, or a... a hatch, it shall be possible to push interior panels or liners out of their retention devices and into the interior of the vehicle after removing the hatch. If emergency roof access is provided by...

  5. 29 CFR 1915.506 - Hazards of fixed extinguishing systems on board vessels and vessel sections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... operational. (d) Doors and hatches. The employer must: (1) Take protective measures to ensure that all doors, hatches, scuttles, and other exit openings remain working and accessible for escape in the event the systems are activated; and (2) Ensure that all inward opening doors, hatches, scuttles, and other...

  6. 49 CFR 238.123 - Emergency roof access.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... inches laterally. (b) Means of access. Emergency roof access shall be provided by means of a hatch, or a... a hatch, it shall be possible to push interior panels or liners out of their retention devices and into the interior of the vehicle after removing the hatch. If emergency roof access is provided by...

  7. 76 FR 32188 - Hatch Solar Energy Center 1, LLC; Supplemental Notice That Initial Market-Based Rate Filing...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-03

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. ER11-3635-000] Hatch Solar Energy Center 1, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for... Hatch Solar Energy Center 1, LLC's application for market-based rate authority, with an accompanying...

  8. 29 CFR 780.211 - Contract production of hatching eggs.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Contract production of hatching eggs. 780.211 Section 780... eggs. It is common practice for hatcherymen to enter into arrangements with farmer poultry raisers for the production of hatching eggs which the hatchery agrees to buy. Ordinarily, the farmer furnishes the...

  9. Breeding bald eagles in captivity

    USGS Publications Warehouse

    Maestrelli, J.R.; Wiemeyer, Stanley N.

    1975-01-01

    A 7-year-old female Bald Eagle from Alabama was paired with a 4-year-old Alaskan male in a large flight pen during December 1969. Both birds were free of physical defects when originally placed in the pen but the female was blind in one eye prior to the 1973 breeding season.....Nesting first occurred during 1971 when at least two eggs were laid; all but one, which showed no sign of embryonic development after being incubated for 56 days, were broken by the adult birds. Two of three eggs laid in 1972 hatched. Both young died a few days after hatching following a period of inclement weather. Three eggs were laid and hatched during 1973. Antagonism between the nestlings was observed soon after hatching and may have been responsible for the unobserved death of one nestling, two days after the third young hatched. The two remaining young were raised by the adult birds and eventually left the nest 85 days after the first egg hatched. Incubation periods for the 1972-73 clutches averaged 35 days. No renesting attempts were made by the eagles during the 3.year period.

  10. Transport stress-induced cerebrum oxidative stress is not mitigated by activating the Nrf2 antioxidant defense response in newly hatched chicks.

    PubMed

    Ge, J; Li, H; Sun, F; Li, X-N; Lin, J; Xia, J; Zhang, C; Li, J-L

    2017-07-01

    Transportation of newly hatched chicks from the hatchery to the farm is inevitable, especially for parent stock and grandsire parent stock chicks. However, the possible effects of transport stress in the newly hatched chicks are poorly understood. The aim of this study was to determine the adaptive responses to transport stress by activing the nuclear factor-erythroid 2-related factor 2 (Nrf2)-induced antioxidant defense. One hundred twenty newly hatched chicks were divided into 3 groups (control group, transport group, and simulation transport group) for 2, 4, and 8 h of real or simulated transportation. Transport stress could cause oxidative stress in the cerebrum of newly hatched chicks by increasing lipid peroxidation and production of free radicals and decreasing the activities of antioxidant enzymes and the glutathione:oxidized glutathione ratio. Transport stress activated the Nrf2 signaling pathway and triggered the transcription of antioxidant parameters. However, transport stress-induced cerebrum oxidative stress was not mitigated by activating the Nrf2 antioxidant defense response in newly hatched chicks.

  11. A Meta-Analysis of Experiments Linking Incubation Conditions with Subsequent Leg Weakness in Broiler Chickens

    PubMed Central

    Groves, Peter J.; Muir, Wendy I.

    2014-01-01

    A series of incubation and broiler growth studies were conducted using one strain of broiler chicken (fast feathering dam line) observing incubation effects on femoral bone ash % at hatch and the ability of the bird to remain standing at 6 weeks of age (Latency-To-Lie). Egg shell temperatures during incubation were consistently recorded. Parsimonious models were developed across eight studies using stepwise multiple linear regression of egg shell temperatures over 3-day periods and both bone ash at hatch and Latency-To-Lie. A model for bone ash at hatch explained 70% of the variation in this factor and revealed an association with lower egg shell temperatures during days 4–6 and 13–15 and higher egg shell temperatures during days 16–18 of incubation. Bone ash at hatch and subsequent Latency-To-Lie were positively correlated (r = 0.57, P<0.05). A model described 66% of the variation Latency-To-Lie showing significant association of the interaction of femoral ash at hatch and lower average egg shell temperatures over the first 15 days of incubation. Lower egg shell temperature in the early to mid incubation process (days 1–15) and higher egg shell temperatures at a later stage (days 16–18) will both tend to delay the hatch time of incubating eggs. Incubation profiles that resulted in later hatching chicks produced birds which could remain standing for a longer time at 6 weeks of age. This supports a contention that the effects of incubation observed in many studies may in fact relate more to earlier hatching and longer sojourn of the hatched chick in the final stage incubator. The implication of these outcomes are that the optimum egg shell temperature during incubation for broiler leg strength development may be lower than that regarded as ideal (37.8°C) for maximum hatchability and chick growth. PMID:25054636

  12. A meta-analysis of experiments linking incubation conditions with subsequent leg weakness in broiler chickens.

    PubMed

    Groves, Peter J; Muir, Wendy I

    2014-01-01

    A series of incubation and broiler growth studies were conducted using one strain of broiler chicken (fast feathering dam line) observing incubation effects on femoral bone ash % at hatch and the ability of the bird to remain standing at 6 weeks of age (Latency-To-Lie). Egg shell temperatures during incubation were consistently recorded. Parsimonious models were developed across eight studies using stepwise multiple linear regression of egg shell temperatures over 3-day periods and both bone ash at hatch and Latency-To-Lie. A model for bone ash at hatch explained 70% of the variation in this factor and revealed an association with lower egg shell temperatures during days 4-6 and 13-15 and higher egg shell temperatures during days 16-18 of incubation. Bone ash at hatch and subsequent Latency-To-Lie were positively correlated (r = 0.57, P<0.05). A model described 66% of the variation Latency-To-Lie showing significant association of the interaction of femoral ash at hatch and lower average egg shell temperatures over the first 15 days of incubation. Lower egg shell temperature in the early to mid incubation process (days 1-15) and higher egg shell temperatures at a later stage (days 16-18) will both tend to delay the hatch time of incubating eggs. Incubation profiles that resulted in later hatching chicks produced birds which could remain standing for a longer time at 6 weeks of age. This supports a contention that the effects of incubation observed in many studies may in fact relate more to earlier hatching and longer sojourn of the hatched chick in the final stage incubator. The implication of these outcomes are that the optimum egg shell temperature during incubation for broiler leg strength development may be lower than that regarded as ideal (37.8°C) for maximum hatchability and chick growth.

  13. Usefulness of HATCH score in the prediction of new-onset atrial fibrillation for Asians.

    PubMed

    Suenari, Kazuyoshi; Chao, Tze-Fan; Liu, Chia-Jen; Kihara, Yasuki; Chen, Tzeng-Ji; Chen, Shih-Ann

    2017-01-01

    The HATCH score (hypertension <1 point>, age >75 years <1 point>, stroke or transient ischemic attack <2 points>, chronic obstructive pulmonary disease <1 point>, and heart failure <2 points>) was reported to be useful for predicting the progression of atrial fibrillation (AF) from paroxysmal to persistent or permanent AF for patients who participated in the Euro Heart Survey. The goal of the current study was to investigate whether the HATCH score was a useful scheme in predicting new-onset AF. Furthermore, we aimed to use the HATCH scoring system to estimate the individual risk in developing AF for patients with different comorbidities. We used the "Taiwan National Health Insurance Research Database." From January 1, 2000, to December 31, 2001, a total of 670,804 patients older than 20 years old and who had no history of cardiac arrhythmias were enrolled. According to the calculation rule of the HATCH score, 599,780 (score 0), 46,661 (score 1), 12,892 (score 2), 7456 (score 3), 2944 (score 4), 802 (score 5), 202 (score 6), and 67 (score 7) patients were studied and followed for the new onset of AF. During a follow-up of 9.0 ± 2.2 years, there were 9174 (1.4%) patients experiencing new-onset AF. The incidence of AF was 1.5 per 1000 patient-years. The incidence increased from 0.8 per 1000 patient-years for patients with a HATCH score of 0 to 57.3 per 1000 patient-years for those with a HATCH score of 7. After an adjustment for the gender and comorbidities, the hazard ratio (95% confidence interval) of each increment of the HATCH score in predicting AF was 2.059 (2.027-2.093; P < 0.001). The HATCH score was useful in risk estimation and stratification of new-onset AF.

  14. Parental genetic material and oxygen concentration affect hatch dynamics of mouse embryo in vitro.

    PubMed

    Zhan, Shaoquan; Cao, Shanbo; Du, Hongzi; Sun, Yuan; Li, Li; Ding, Chenhui; Zheng, Haiyan; Huang, Junjiu

    2018-04-21

    Hatching is crucial for mammalian embryo implantation, since difficulties during this process can lead to implantation failure, ectopic pregnancy and consequent infertility. Despite years of intensive researches, how internal and external factors affecting embryo hatch are still largely unclear. The effects of parental genetic material and oxygen concentration on hatch process were examined. Fertilized and parthenogenetic mouse preimplantation embryos were cultured in vitro under 5 and 20% oxygen for 120 h. Zona pellucida drilling by Peizo micromanipulation were performed to resemble the breach by sperm penetration. Firstly, parthenogenetic embryos had similarly high blastocyst developmental efficiency as fertilized embryos, but significantly higher hatch ratio than fertilized embryos in both O 2 concentrations. 5% O 2 reduced the hatch rate of fertilized embryos from 58.2 to 23.8%, but increased that of parthenogenetic embryos from 81.2 to 90.8% significantly. Analogously, 5% O 2 decreased the ratio of Oct4-positive cells in fertilized blastocysts, whereas increased that in parthenogenetic blastocysts. Additionally, 5% O 2 increased the total embryonic cell number in both fertilized and parthegenetic embryos, when compared to 20% O 2 , and the total cell number of fertilized embryos was also higher than that of parthegenetic embryos, despite O 2 concentration. Real-time PCR revealed that the expression of key genes involving in MAPK pathway and superoxide dismutase family might contribute to preimplantation development and consequent blastocyst hatch in vitro. Finally, we showed that fertilized and parthenogenetic embryos have diverse hatch dynamics in vitro, although the zona pellucida integrity is not the main reason for their mechanistic differences. Both parental genetic material and O 2 concentration, as the representative of intrinsic and extrinsic factors respectively, have significant impacts on mouse preimplantation development and subsequent hatch dynamics, probably by regulating the gene expression involving in MAPK pathway and superoxide dismutase family to control embryonic cell proliferation and allocation of ICM cells.

  15. Comparison of CHA2DS2-VASc, CHADS2 and HATCH scores for the prediction of new-onset atrial fibrillation in cancer patients: A nationwide cohort study of 760,339 study participants with competing risk analysis.

    PubMed

    Hu, Wei-Syun; Lin, Cheng-Li

    2017-11-01

    The current study was conducted to assess the ability of CHA 2 DS 2 -VASc, CHADS 2 and HATCH scores in predicting new-onset atrial fibrillation (AF) among patients with cancer. Patients with newly diagnosed cancer between 1 January, 2000 and 31 December, 2011, from the Registry for Catastrophic Illness Patient Database, were defined as the study cohort. CHA 2 DS 2 -VASc, CHADS 2 and HATCH scores were used for new-onset AF prediction in these study patients, and the predictive accuracy of the scores was assessed by the receiver operating characteristics (ROC) curve. A total of 760,339 cancer patients were identified as the study participants. The ROC curves were 0.68 (95% confidence interval [CI] = 0.68-0.69) for the CHA 2 DS 2 -VASc score, 0.67 (95% CI = 0.67-0.68) for the CHADS 2 score and 0.69 (95% CI = 0.69-0.70) for the HATCH score. There were significant differences of c-statistics among CHA 2 DS 2 -VASc score, CHADS 2 score and HATCH score (CHA 2 DS 2 -VASc score vs. CHADS 2 score, p = 0.01; CHA 2 DS 2 -VASc score vs. HATCH score, p = 0.002; CHADS 2 score vs. HATCH score, p < 0.001). The current study is the first to assess the prognostic value of 3 AF risk scores (CHA 2 DS 2 -VASc, CHADS 2 and HATCH scores) in patients with newly-diagnosed cancer. HATCH score was found to have a slightly but significantly better predictive performance than the other 2 scores. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Enhancing hatch rate and survival in laboratory-reared hybrid Devils Hole Pupfish through application of antibiotics to eggs and larvae

    USGS Publications Warehouse

    Feuerbacher, Olin; Bonar, Scott A.; Barrett, Paul J.

    2017-01-01

    We evaluated the effectiveness of four antibiotics in enhancing the hatch rate, larval survival, and adult survival of hybrid Devils Hole Pupfish Cyprinodon diabolis (hybridized with Ash Meadows Amargosa Pupfish C. nevadensis mionectes). Cephalexin (CEX; concentration = 6.6 mg/L of water), chloramphenicol (CAM; 50 mg/L), erythromycin (ERY; 12.5 mg/L), and trimethoprim sulfamethoxazole (TMP-SMX; 25 mg/L) were applied as a constant bath either to incubating eggs or to larvae that hatched from untreated eggs. Hatch rate was roughly doubled by incubation in the presence of CAM (68% hatch) and TMP-SMX (66%) relative to the control (28%). Cephalexin and ERY conferred no benefit upon the hatch rate. Among fry that hatched from treated eggs, there was no increase in 15-d larval survival. However, fish that hatched from eggs treated with CAM, ERY, and TMP-SMX demonstrated enhanced survival at 360 d (51.2, 38.4, and 43.6%, respectively) and at 540 d (22.6, 6.8, and 20.2%, respectively); the untreated control had no survivors to those time points. All groups of eggs treated with antibiotics showed reductions in bacterial colony-forming units (CFUs) at 24 h posttreatment. At 120 h posttreatment, CEX-treated eggs had CFU counts similar to those of the control, whereas the TMP-SMX-treated eggs had the lowest CFU counts. Eggs treated with CAM and ERY had similar CFU counts, which were significantly reduced from the control counts. Larvae that were treated with CAM and TMP-SMX within 12 h posthatch showed enhanced 15-d survival (74% and 72%, respectively) in comparison with the control (56%). For pupfish rearing efforts in which antibiotic use is appropriate, CAM and TMP-SMX appear to provide the greatest benefit, particularly when applied to incubating eggs rather than to hatched larvae.

  17. Effects of male removal on female reproductive biology in Ross' and Lesser Snow Geese

    USGS Publications Warehouse

    Leschack, C.R.; Afton, A.D.; Alisauskas, R.T.

    1998-01-01

    We studied effects of mate removal on nesting and hatching success, incubation behavior, body mass, and post-hatch dispersal distance of female Ross' (Chen rossii) and Lesser Snow Geese (C. caerulescens caerulescens) at Karrak Lake. N.W.T., Canada. Male ge and widowed and paired control females were monitored through post-hatch dispersal. Nesting and hatching success did not differ between species or treatments (widowed vs paired) and averaged 77.5 ?? 3.8% and 64.0 ?? 3.6% (??SE), respectively. Paired females spent more time with their bills tucked (23.7 ?? 3.3% vs 9.1 ?? 4.0%) and less time alert (8.6 ?? 2.9% vs 22.9 ?? 3.5%) while on nests than did widowed females. Snow widowed females (31.1 ?? 4.7%) and Ross' widowed females (20.6 ?? 6.0%) generally spent more time each day in head-up alert than did Snow paired females (7.1 ?? 3.8%). Snow paired maleS (11.8 ?? 3.8%), Ross' paired females (9.4 ?? 3.6%), and Ross' paired males (7.9 ?? 3.6%). Body mass of paired and widowed female Ross' Geese did not differ at hatch or at time of post-hatch recapture; however, mean distance recaptured from the breeding colony was greater for paired (50.9 ?? 6.1 km) than for widowed females (27.3 ?? 6.6 km). Total mass gain (276 ?? 19 g) and rate of mass gain (8.4 ?? 0.5 g/day), from hatch until post-hatch recapture (33.1 ?? 1.2 days), were similar for widowed and paired female Ross' Geese. Male removal experiments in monogamous, precocial species generally have produced few effects on female nesting success or incubation behavior. We suggest that male parental care in arctic-nesting geese is more critical during laying and the post-hatch period than during incubation.

  18. The hatching larva of the priapulid worm Halicryptus spinulosus

    PubMed Central

    Janssen, Ralf; Wennberg, Sofia A; Budd, Graham E

    2009-01-01

    Despite their increasing evolutionary importance, basic knowledge about the priapulid worms remains limited. In particular, priapulid development has only been partially documented. Following previous description of hatching and the earliest larval stages of Priapulus caudatus, we here describe the hatching larva of Halicryptus spinulosus. Comparison of the P. caudatus and the H. spinulosus hatching larvae allows us to attempt to reconstruct the ground pattern of priapulid development. These findings may further help unravelling the phylogenetic position of the Priapulida within the Scalidophora and hence contribute to the elucidation of the nature of the ecdysozoan ancestor. PMID:19470151

  19. The hatching larva of the priapulid worm Halicryptus spinulosus.

    PubMed

    Janssen, Ralf; Wennberg, Sofia A; Budd, Graham E

    2009-05-26

    Despite their increasing evolutionary importance, basic knowledge about the priapulid worms remains limited. In particular, priapulid development has only been partially documented. Following previous description of hatching and the earliest larval stages of Priapulus caudatus, we here describe the hatching larva of Halicryptus spinulosus. Comparison of the P. caudatus and the H. spinulosus hatching larvae allows us to attempt to reconstruct the ground pattern of priapulid development. These findings may further help unravelling the phylogenetic position of the Priapulida within the Scalidophora and hence contribute to the elucidation of the nature of the ecdysozoan ancestor.

  20. Cold storage effects on egg hatch in laboratory-reared Culicoides variipennis sonorensis (Diptera: Ceratopogonidae).

    PubMed

    Hunt, G J; Tabachnick, W J

    1995-09-01

    The effects of cold storage (5 degrees C) on the hatching rates of laboratory-reared Culicoides variipennis sonorensis eggs were examined. Mortality increased with storage time. Average maximum embryo survivorship for 4 trials was 55.0 +/- 4.2 (+/- SEM) days. Alternating daily cycles of high and then low mean hatching rates occurred and possibly were due to location differences in temperature within the temperature-controlled rearing system. During cold storage at 5 degrees C, C. v. sonorensis eggs may be kept for ca. 28 days with an anticipated hatching rate of about 50%.

  1. Sex Differences in Brain Thyroid Hormone Levels during Early Post-Hatching Development in Zebra Finch (Taeniopygia guttata).

    PubMed

    Yamaguchi, Shinji; Hayase, Shin; Aoki, Naoya; Takehara, Akihiko; Ishigohoka, Jun; Matsushima, Toshiya; Wada, Kazuhiro; Homma, Koichi J

    2017-01-01

    Thyroid hormones are closely linked to the hatching process in precocial birds. Previously, we showed that thyroid hormones in brain had a strong impact on filial imprinting, an early learning behavior in newly hatched chicks; brain 3,5,3'-triiodothyronine (T3) peaks around hatching and imprinting training induces additional T3 release, thus, extending the sensitive period for imprinting and enabling subsequent other learning. On the other hand, blood thyroid hormone levels have been reported to increase gradually after hatching in altricial species, but it remains unknown how the brain thyroid hormone levels change during post-hatching development of altricial birds. Here, we determined the changes in serum and brain thyroid hormone levels of a passerine songbird species, the zebra finch using radioimmunoassay. In the serum, we found a gradual increase in thyroid hormone levels during post-hatching development, as well as differences between male and female finches. In the brain, there was clear surge in the hormone levels during development in males and females coinciding with the time of fledging, but the onset of the surge of thyroxine (T4) in males preceded that of females, whereas the onset of the surge of T3 in males succeeded that of females. These findings provide a basis for understanding the functions of thyroid hormones during early development and learning in altricial birds.

  2. 75 FR 44935 - Notice of Request for Extension of Approval of an Information Collection; Certificate for Poultry...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-30

    ... Hatching Eggs for Export AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Extension of... poultry hatching eggs from the United States. DATES: We will consider all comments that we receive on or... FURTHER INFORMATION CONTACT: For information on the export of poultry and poultry hatching eggs from the...

  3. 78 FR 58267 - Notice of Request for Extension of Approval of an Information Collection; Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-23

    ... Hatching Eggs for Export AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Extension of... hatching eggs from the United States. DATES: We will consider all comments that we receive on or before... and hatching eggs from the United States, contact Dr. Antonio Ramirez, Senior Staff Veterinarian, NCIE...

  4. 78 FR 19080 - Importation of Live Birds and Poultry, Poultry Meat, and Poultry Products From a Region in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ... from the APHIS-defined EU poultry trade region of hatching eggs under official seal, including those... and poultry, including hatching eggs, and poultry meat and products from the APHIS-defined EU poultry... of carcasses, parts of products of carcasses, and eggs (other than hatching eggs) \\1\\ of poultry...

  5. The influence of predator threat on the timing of a life-history switch point: predator-induced hatching in the southern leopard frog (Rana sphenocephala)

    Treesearch

    James B. Johnson; Daniel Saenz; Cory K. Adams; Richard N. Conner

    2003-01-01

    Abstract: We tested the hypotheses that potential egg predators, crayfish Procambarus nigrocinctus and dytiscid Cybister sp. larvae, would accelerate the timing of hatching and that a larval predator, dragonfly naiad Anax junius, would delay hatching in the southern leopard frog (Rana...

  6. 75 FR 62560 - Exemption and Equivalent Arrangements Under the International Convention on Load Lines, 1966, as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-12

    ... equivalent arrangements, as permitted by the ICLL and U.S. regulations, regarding hatch covers for hopper... Guard issued formal notification to the IMO of equivalent arrangements for hatch covers for certain... arrangements for hatch covers for certain manned, self-propelled open hopper dredges on November 12, 2009. We...

  7. ODS/Mir hatch opening during STS-89 mission

    NASA Image and Video Library

    2009-09-22

    STS089-349-021 (22-31 Jan 1998) --- Cosmonaut Pavel Vinogradov, flight engineer for the Mir-24 crew, peers through the hatch, from the inside of the Russia?s Mir Space Station, at arriving Space Shuttle Endeavour members of the STS-89 crew prior to hatch opening. STS-89/Mir-24 marked the eighth of nine Shuttle/Mir dockings.

  8. 5 CFR 1800.1 - Filing complaints of prohibited personnel practices or other prohibited activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... allegations of the following prohibited activities: (1) Violation of the Federal Hatch Act at title 5 of the U.S. Code, chapter 73, subchapter III; (2) Violation of the state and local Hatch Act at title 5 of... alleging prohibited personnel practices or other prohibited activities (other than the Hatch Act). (1...

  9. 14. VIEW OF NORTHSOUTH ROAD WHICH PARALLELS ROAD TO HATCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. VIEW OF NORTH-SOUTH ROAD WHICH PARALLELS ROAD TO HATCH ADIT (FEATURE B-28). NOTE MODERN 'LAY DOWN' FENCE ON ROAD. ROAD LIES TO THE WEST OF THE HATCH ADIT AND PHOTOGRAPH IS VIEW TO THE SOUTH. (OCTOBER, 1995) - Nevada Lucky Tiger Mill & Mine, East slope of Buckskin Mountain, Paradise Valley, Humboldt County, NV

  10. 5 CFR 1800.1 - Filing complaints of prohibited personnel practices or other prohibited activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... allegations of the following prohibited activities: (1) Violation of the Federal Hatch Act at title 5 of the U.S. Code, chapter 73, subchapter III; (2) Violation of the state and local Hatch Act at title 5 of... alleging prohibited personnel practices or other prohibited activities (other than the Hatch Act). (1...

  11. 5 CFR 1800.1 - Filing complaints of prohibited personnel practices or other prohibited activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... allegations of the following prohibited activities: (1) Violation of the Federal Hatch Act at title 5 of the U.S. Code, chapter 73, subchapter III; (2) Violation of the state and local Hatch Act at title 5 of... alleging prohibited personnel practices or other prohibited activities (other than the Hatch Act). (1...

  12. 77 FR 26659 - Political Activity-Federal Employees Residing in Designated Localities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ... partial exemption from the political activity restrictions in the Hatch Act, and to add King George County... George County meets the criteria in the Hatch Act and OPM regulations for a partial exemption to issue... INFORMATION: The Hatch Act, at 5 U.S.C. 7323(a)(2) and (3), prohibits Federal employees from becoming...

  13. 5 CFR 1800.1 - Filing complaints of prohibited personnel practices or other prohibited activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... allegations of the following prohibited activities: (1) Violation of the Federal Hatch Act at title 5 of the U.S. Code, chapter 73, subchapter III; (2) Violation of the state and local Hatch Act at title 5 of... alleging prohibited personnel practices or other prohibited activities (other than the Hatch Act). (1...

  14. 5 CFR 1800.1 - Filing complaints of prohibited personnel practices or other prohibited activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... allegations of the following prohibited activities: (1) Violation of the Federal Hatch Act at title 5 of the U.S. Code, chapter 73, subchapter III; (2) Violation of the state and local Hatch Act at title 5 of... alleging prohibited personnel practices or other prohibited activities (other than the Hatch Act). (1...

  15. Microscopic morphology and apoptosis of ovarian tissue after cryopreservation using a vitrification method in post-hatching turkey poults, Meleagris gallopavo

    USDA-ARS?s Scientific Manuscript database

    1. Microscopic morphology of ovarian tissue in post-hatching turkey poults at various ages was investigated. 2. Hematoxylin and eosin staining were used and the diameter of the oocytes and follicles were measured using microphotography. 3. Immediately after hatching, oocytes in one-day turkey pou...

  16. Survey of hatching spines of bee larvae including those of apis mellifera (Hymenoptera: Apoidea)

    USDA-ARS?s Scientific Manuscript database

    This paper explores the occurrence of hatching spines among bee taxa and how these structures enable a larva on hatching to extricate itself from the egg chorion. These spines, arranged in a linear sequence along the sides of the first instar just dorsad to the spiracles, have been observed and reco...

  17. Inheritance and world variation in thermal requirements for egg hatch in Lymantria dispar (Lepidoptera: Erebidae)

    Treesearch

    M.A. Keena

    2016-01-01

    Mode of inheritance of hatch traits in Lymantria dispar L. was determined by crossing populations nearly fixed for the phenotypic extremes. The nondiapausing phenotype was inherited via a single recessive gene and the phenotype with reduced low temperature exposure requirements before hatch was inherited via a single dominant gene. There was no...

  18. In ovo feeding of L-arginine alters energy metabolism in post-hatch broilers.

    PubMed

    Yu, L L; Gao, T; Zhao, M M; Lv, P A; Zhang, L; Li, J L; Jiang, Y; Gao, F; Zhou, G H

    2018-01-01

    This study aimed to investigate the effects of in ovo feeding (IOF) of L-arginine (Arg) on energy metabolism in post-hatch broilers. A total of 720 eggs was randomly assigned to 3 treatments: 1) non-injected control group, 2) 0.75% NaCl diluent-injected control group, and 3) 1.0% Arg solution-injected group. At 17.5 d of incubation, 0.6 mL of each solution was injected into the amniotic fluid of each egg of injected groups. After hatching, 80 male chicks were randomly assigned to each treatment group with 8 replicates per group. The results showed that IOF of Arg increased glycogen and glucose concentrations in the liver and pectoral muscle of broilers at hatch (P < 0.05). The plasma glucose and insulin levels were higher in the Arg group than in the non-injected and diluent-injected control groups (P < 0.05). Meanwhile, IOF of Arg enhanced the hepatic glucose-6-phosphatase (G6P) activity at hatch (P < 0.05). There was no difference in hexokinase (HK) or phosphofructokinase (PFK) enzyme activities in the pectoral muscle in all groups. Further, IOF of Arg increased the phosphoenolpyruvate carboxykinase (PEPCK) and fructose-1,6-bisphosphatase (FBP) mRNA expressions at hatch (P < 0.05). In addition, broilers in the Arg group had a higher mRNA expression of glycogen synthase and a lower expression of glycogen phosphorylase in the liver and pectoral muscles than in the non-injected controls at hatch (P < 0.05). In conclusion, IOF of Arg solution enhanced liver and pectoral muscle energy reserves at hatch, which might be considered as an effective strategy for regulating early energy metabolism in broilers. © 2017 Poultry Science Association Inc.

  19. Laser assisted zona hatching does not lead to immediate impairment in human embryo quality and metabolism.

    PubMed

    Uppangala, Shubhashree; D'Souza, Fiona; Pudakalakatti, Shivanand; Atreya, Hanudatta S; Raval, Keyur; Kalthur, Guruprasad; Adiga, Satish Kumar

    2016-12-01

    Laser assisted zona hatching (LAH) is a routinely used therapeutic intervention in assisted reproductive technology for patients with poor prognosis. However, results are not conclusive in demonstrating the benefits of zona hatching in improving the pregnancy rate. Recent observations on LAH induced genetic instability in animal embryos prompted us to look into the effects of laser assisted zona hatching on the human preimplantation embryo quality and metabolic uptake using high resolution nuclear magnetic resonance (NMR) technology. This experimental prospective study included fifty embryos from twenty-five patients undergoing intra cytoplasmic sperm injection. Embryo quality assessment followed by profiling of spent media for the non-invasive evaluation of metabolites was performed using NMR spectroscopy 24 hours after laser treatment and compared with that of non-treated sibling embryos. Both cell number and embryo quality on day 3 of development did not vary significantly between the two groups at 24 hours post laser treatment interval. Time lapse monitoring of the embryos for 24 hours did not reveal blastomere fragmentation adjacent to the point of laser treatment. Similarly, principal component analysis of metabolites did not demonstrate any variation across the groups. These results suggest that laser assisted zona hatching does not affect human preimplantation embryo morphology and metabolism at least until 24 hours post laser assisted zona hatching. However, studies are required to elucidate laser induced metabolic and developmental changes at extended time periods. AH: assisted hatching; ART: assisted reproductive technology; DNA: deoxy-ribo nucleic acid; LAH: laser assisted hatching; MHz: megahertz; NMR: nuclear magnetic resonance; PCA: principal component analysis; PGD: preimplantation genetic diagnosis; TLM: time lapse monitoring.

  20. Nesting ecology of Podocnemis expansa (Schweigger, 1812) and Podocnemis unifilis (Troschel, 1848) (Testudines, Podocnemididae) in the Javaés River, Brazil.

    PubMed

    Ferreira Júnior, P D; Castro, P T A

    2010-02-01

    Nest site has influence on incubation duration and hatching success of two Neotropical turtles, the giant Amazon River turtle (Podocnemis expansa) and yellow-spotted side-neck turtle (Podocnemis unifilis--'Tracajá'). The 2000 and 2001 nesting seasons have been monitored at the Javaés River in Bananal Island, Brazil. Although they nest on the same beaches, there is a separation of the nesting areas of P unifilis and P. expansa nests on the upper parts of the beach. The incubation duration for P. expansa is influenced by the nesting period, the height of the nest from the river, the clutch size, and the grain size in the site of the nest. Nests of Podocnemis expansa placed in coarse sediments have shorter incubation duration than those placed in finer sediments. The hatching success in P. expansa is influenced by grain size, incubation duration, and nesting period. The grain size is negatively correlated with hatching success, indicating that the nests situated in finer-grained sand have better chances of successful egg hatching than those in coarser-grained sand. Nests of the end of the reproductive season have lower hatching success and incubation duration than those at the start of the season. For P. unifilis, the nesting period and nest depth influence the incubation duration; moreover, the river dynamics significantly affect the hatching success. The oscillation of the river level and the moment of initial increase, the height of the nest from the river level, and the nesting period are all decisive components for hatching success. The results of this research show the importance of protecting areas with great geological diversity, wherein the features of the environment can affect the microenvironment of nests, with consequences on incubation duration and hatching success.

  1. Ventilation changes associated with hatching and maturation of an endothermic phenotype in the Pekin duck, Anas platyrhynchos domestica.

    PubMed

    Sirsat, Tushar S; Dzialowski, Edward M

    2016-04-15

    Precocial birds begin embryonic life with an ectothermic metabolic phenotype and rapidly develop an endothermic phenotype after hatching. Switching to a high-energy, endothermic phenotype requires high-functioning respiratory and cardiovascular systems to deliver sufficient environmental oxygen to the tissues. We measured tidal volume (VT), breathing frequency (ƒ), minute ventilation (V̇e), and whole-animal oxygen consumption (V̇o2) in response to gradual cooling from 37.5°C (externally pipped paranates, EP) or 35°C (hatchlings) to 20°C along with response to hypercapnia during developmental transition from an ectothermic, EP paranate to endothermic hatchling. To examine potential eggshell constraints on EP ventilation, we repeated these experiments in artificially hatched early and late EP paranates. Hatchlings and artificially hatched late EP paranates were able to increase V̇o2significantly in response to cooling. EP paranates had high ƒ that decreased with cooling, coupled with an unchanging low VT and did not respond to hypercapnia. Hatchlings had significantly lower ƒ and higher VT and V̇e that increased with cooling and hypercapnia. In response to artificial hatching, all ventilation values quickly reached those of hatchlings and responded to hypercapnia. The timing of artificial hatching influenced the temperature response, with only artificially hatched late EP animals, exhibiting the hatchling ventilation response to cooling. We suggest one potential constraint on ventilatory responses of EP paranates is the rigid eggshell, limiting air sac expansion during inhalation and constraining VT Upon natural or artificial hatching, the VT limitation is removed and the animal is able to increase VT, V̇e, and thus V̇o2, and exhibit an endothermic phenotype. Copyright © 2016 the American Physiological Society.

  2. Ventilation changes associated with hatching and maturation of an endothermic phenotype in the Pekin duck, Anas platyrhynchos domestica

    PubMed Central

    Sirsat, Tushar S.

    2016-01-01

    Precocial birds begin embryonic life with an ectothermic metabolic phenotype and rapidly develop an endothermic phenotype after hatching. Switching to a high-energy, endothermic phenotype requires high-functioning respiratory and cardiovascular systems to deliver sufficient environmental oxygen to the tissues. We measured tidal volume (VT), breathing frequency (ƒ), minute ventilation (V̇e), and whole-animal oxygen consumption (V̇o2) in response to gradual cooling from 37.5°C (externally pipped paranates, EP) or 35°C (hatchlings) to 20°C along with response to hypercapnia during developmental transition from an ectothermic, EP paranate to endothermic hatchling. To examine potential eggshell constraints on EP ventilation, we repeated these experiments in artificially hatched early and late EP paranates. Hatchlings and artificially hatched late EP paranates were able to increase V̇o2 significantly in response to cooling. EP paranates had high ƒ that decreased with cooling, coupled with an unchanging low VT and did not respond to hypercapnia. Hatchlings had significantly lower ƒ and higher VT and V̇e that increased with cooling and hypercapnia. In response to artificial hatching, all ventilation values quickly reached those of hatchlings and responded to hypercapnia. The timing of artificial hatching influenced the temperature response, with only artificially hatched late EP animals, exhibiting the hatchling ventilation response to cooling. We suggest one potential constraint on ventilatory responses of EP paranates is the rigid eggshell, limiting air sac expansion during inhalation and constraining VT. Upon natural or artificial hatching, the VT limitation is removed and the animal is able to increase VT, V̇e, and thus V̇o2, and exhibit an endothermic phenotype. PMID:26818053

  3. Within-female plasticity in sex allocation is associated with a behavioural polyphenism in house wrens.

    PubMed

    Bowers, E K; Thompson, C F; Sakaluk, S K

    2016-03-01

    Sex allocation theory assumes individual plasticity in maternal strategies, but few studies have investigated within-individual changes across environments. In house wrens, differences between nests in the degree of hatching synchrony of eggs represent a behavioural polyphenism in females, and its expression varies with seasonal changes in the environment. Between-nest differences in hatching asynchrony also create different environments for offspring, and sons are more strongly affected than daughters by sibling competition when hatching occurs asynchronously over several days. Here, we examined variation in hatching asynchrony and sex allocation, and its consequences for offspring fitness. The number and condition of fledglings declined seasonally, and the frequency of asynchronous hatching increased. In broods hatched asynchronously, sons, which are over-represented in the earlier-laid eggs, were in better condition than daughters, which are over-represented in the later-laid eggs. Nonetheless, asynchronous broods were more productive later within seasons. The proportion of sons in asynchronous broods increased seasonally, whereas there was a seasonal increase in the production of daughters by mothers hatching their eggs synchronously, which was characterized by within-female changes in offspring sex and not by sex-biased mortality. As adults, sons from asynchronous broods were in better condition and produced more broods of their own than males from synchronous broods, and both males and females from asynchronous broods had higher lifetime reproductive success than those from synchronous broods. In conclusion, hatching patterns are under maternal control, representing distinct strategies for allocating offspring within broods, and are associated with offspring sex ratios and differences in offspring reproductive success. © 2015 European Society For Evolutionary Biology. Journal of Evolutionary Biology © 2015 European Society For Evolutionary Biology.

  4. Effect of simultaneous variation in temperature and ammonia concentration on percent fertilization and hatching in Crassostrea ariakensis.

    PubMed

    Hui, Wang; Jiahui, Liu; Hongshuai, Yang; Jin, Liu; Zhigang, Liu

    2014-04-01

    The combined effects of temperature and ammonia concentration on the percent fertilization and percent hatching in Crassostrea ariakensis were examined under laboratory conditions using the central composite design and response surface methodology. The results indicated: (1) The linear effects of temperature and ammonia concentration on the percent fertilization were significant (P<0.05), and the quadratic effects were highly significant (P<0.01). The interactive effect between temperature and ammonia concentration on the percent fertilization was not significant (P>0.05). (2) The linear effect of temperature on the percent hatching was highly significant (P<0.01), and that of ammonia concentration was nonsignificant (P>0.05). The quadratic effects of temperature and ammonia concentration on the percent hatching were highly significant (P<0.01). The interaction on the percent hatching was not significant (P>0.05). Temperature was more important than ammonia in influencing the fertilization and hatching in C. ariakensis. (3) The model equations of the percent fertilization and hatching towards temperature and ammonia concentration were established, with the coefficients of determination R(2)=99.4% and 99.76%, respectively. Through the lack-of-fit test, these models were of great adequacy. The predictive coefficients of determination for the two model equations were as high as 94.6% and 98.03%, respectively, showing that they could be used for practical projection. (4) Via the statistical simultaneous optimization technique, the optimal factor level combination, i.e., 25°C/0.038mgmL(-1), was derived, at which the greatest percent fertilization 95.25% and hatching 83.26% was achieved, with the desirability being 97.81%. Our results may provide advantageous guidelines for the successful reproduction of C. ariakensis. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Development of the hypothalamus and pituitary in platypus (Ornithorhynchus anatinus) and short-beaked echidna (Tachyglossus aculeatus).

    PubMed

    Ashwell, Ken W S

    2012-07-01

    The living monotremes (platypus and echidnas) are distinguished by the development of their young in a leathery-shelled egg, a low and variable body temperature and a primitive teat-less mammary gland. Their young are hatched in an immature state and must deal with the external environment, with all its challenges of hypothermia and stress, as well as sourcing nutrients from the maternal mammary gland. The Hill and Hubrecht embryological collections have been used to follow the structural development of the monotreme hypothalamus and its connections with the pituitary gland both in the period leading up to hatching and during the lactational phase of development, and to relate this structural maturation to behavioural development. In the incubation phase, development of the hypothalamus proceeds from closure of the anterior neuropore to formation of the lateral hypothalamic zone and putative medial forebrain bundle. Some medial zone hypothalamic nuclei are emerging at the time of hatching, but these are poorly differentiated and periventricular zone nuclei do not appear until the first week of post-hatching life. Differentiation of the pituitary is also incomplete at hatching, epithelial cords do not develop in the pars anterior until the first week, and the hypothalamo-neurohypophyseal tract does not appear until the second week of post-hatching life. In many respects, the structure of the hypothalamus and pituitary of the newly hatched monotreme is similar to that seen in newborn marsupials, suggesting that both groups rely solely on lateral hypothalamic zone nuclei for whatever homeostatic mechanisms they are capable of at birth/hatching. © 2012 The Author. Journal of Anatomy © 2012 Anatomical Society.

  6. Intraspecific variation in reproductive traits of burrowing owls

    USGS Publications Warehouse

    Conway, Meaghan; Nadeau, Christopher P.; Conway, Courtney J.

    2012-01-01

    Reviews of hatching asynchrony in birds recommended more studies on intraspecific variation in the extent of hatching asynchrony. We examined intraspecific variation in clutch size, laying chronology, onset of incubation, incubation period, and hatching asynchrony in burrowing owls (Athene cunicularia) in the Imperial Valley of California. Mean clutch size was 7.4 eggs and owls averaged 0.5 eggs laid per day. Females varied considerably in laying interval and onset of incubation (range = 1st to 9th egg in the clutch). The mean incubation period was 21.9 days. Hatching interval also varied greatly among females (x = 0.8, range 0.1-2.0 days between successively hatched eggs). Past burrowing owl studies have largely overlooked the substantial intraspecific variation in these traits or have reported estimates that differ from ours. Future studies designed to identify the environmental factors that explain the large intraspecific variation in these traits will likely provide insights into the constraints on local abundance.

  7. 9 CFR 94.6 - Carcasses, meat, parts or products of carcasses, and eggs (other than hatching eggs) of poultry...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... carcasses, and eggs (other than hatching eggs) of poultry, game birds, or other birds; importations from... Carcasses, meat, parts or products of carcasses, and eggs (other than hatching eggs) of poultry, game birds... including meat, of poultry, game birds, or other birds that were raised or slaughtered in any region where...

  8. 9 CFR 94.6 - Carcasses, parts or products of carcasses, and eggs (other than hatching eggs) of poultry, game...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... carcasses, and eggs (other than hatching eggs) of poultry, game birds, or other birds; importations from... § 94.6 Carcasses, parts or products of carcasses, and eggs (other than hatching eggs) of poultry, game... considered to exist. Carcasses, and parts or products of carcasses, of poultry, game birds, or other birds...

  9. 9 CFR 94.6 - Carcasses, parts or products of carcasses, and eggs (other than hatching eggs) of poultry, game...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... carcasses, and eggs (other than hatching eggs) of poultry, game birds, or other birds; importations from... § 94.6 Carcasses, parts or products of carcasses, and eggs (other than hatching eggs) of poultry, game... considered to exist. Carcasses, and parts or products of carcasses, of poultry, game birds, or other birds...

  10. 9 CFR 94.6 - Carcasses, meat, parts or products of carcasses, and eggs (other than hatching eggs) of poultry...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... carcasses, and eggs (other than hatching eggs) of poultry, game birds, or other birds; importations from... Carcasses, meat, parts or products of carcasses, and eggs (other than hatching eggs) of poultry, game birds... including meat, of poultry, game birds, or other birds that were raised or slaughtered in any region where...

  11. 9 CFR 82.8 - Interstate movement of eggs, other than hatching eggs, from a quarantined area.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement of eggs, other than hatching eggs, from a quarantined area. 82.8 Section 82.8 Animals and Animal Products ANIMAL AND... Disease (END) § 82.8 Interstate movement of eggs, other than hatching eggs, from a quarantined area. (a...

  12. Whitson prepares to close PMA2 hatch

    NASA Image and Video Library

    2007-11-04

    S120-E-008857 (4 Nov. 2007) --- Astronaut Peggy Whitson, Expedition 16 commander, prepares to close the hatch in the Pressurized Mating Adapter (PMA-2) of the International Space Station after the STS-120 crewmembers boarded Space Shuttle Discovery for their return trip home. Hatches were closed between the station and the shuttle at 2:03 p.m. (CST) on Nov. 4.

  13. 76 FR 56791 - Notice of Availability of Record of Decision for the Tropic To Hatch (Garkane) 138 kV...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-14

    ... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLUT0300000 L17110000 DT0000 24 1A] Notice of Availability of Record of Decision for the Tropic To Hatch (Garkane) 138 kV Transmission Line... Hatch (Garkane) 138 kilovolt (kV) Transmission Line Environmental Impact Statement (EIS) and the...

  14. 78 FR 35641 - Notice of Intent To Prepare an Amendment to the Mimbres Resource Management Plan and Associated...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    .... SUPPLEMENTARY INFORMATION: The Village of Hatch, New Mexico, has proposed to acquire 339.89 acres of BLM... park and would give the Village of Hatch the ability to enhance its economic opportunities and provide... disposed of in order to promote economic opportunities and community expansion for the Village of Hatch...

  15. The role of behavior in the dispersal of newly hatched gypsy moth larvae

    Treesearch

    Michael L. Mcmanus; Michael L. Mcmanus

    1973-01-01

    Newly hatched gypsy moth larvae are morphologically and behaviorally adapted for airborne dispersal. The diel periodicity of both hatching and dispersal from the egg mass and photopositive behavior assure that larvae are in optimal position for dispersal when air turbulence is maximal at midday. The rate of larval activity depends upon ambient temperature and relative...

  16. 9 CFR 82.8 - Interstate movement of eggs, other than hatching eggs, from a quarantined area.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement of eggs, other than hatching eggs, from a quarantined area. 82.8 Section 82.8 Animals and Animal Products ANIMAL AND... Disease (END) § 82.8 Interstate movement of eggs, other than hatching eggs, from a quarantined area. (a...

  17. Notes on the hatching phases and the size of the juveniles in the Snake-eyed skink Ablepharus kitaibelii (Bibron & Bory de Saint-Vincent, 1833)

    NASA Astrophysics Data System (ADS)

    Vergilov, Vladislav; Natchev, Nikolay

    2018-03-01

    Most of the studies dealing with the Snake-eyed skink (Ablepharus kitaibelii) treat predominantly aspects of the distribution and taxonomy of the species. In the present study we provide information concerning the mechanism of hatching in the Snake-eyed skink and the dimensions of the hatched specimens. We collected data from wild animals, as well as from indoor incubated juveniles. The present study provides the first data concerning the weight of the newly hatched juveniles of the species and discuss on potential misleading data concerning the size of the juveniles in A. kitaibelii.

  18. Space Shuttle Orbiter Crew Hatch Jettison Test using a 0.0405-scale model (16-0) in the Texas A/M low speed wind tunnel (OA362). Space Shuttle aerothermodynamic data report

    NASA Technical Reports Server (NTRS)

    Mitchell, C. E.

    1992-01-01

    This report contains post-test information for the Space Shuttle Orbiter Crew Hatch Jettison Test OA362 which was conducted in the Texas A&M Low Speed Wind Tunnel from 6/15/87 to 6/22/87. The test objective was to verify that the crew hatch, once jettisoned, would clear the orbiter under various simulated flight conditions. Several model hatches were used with the 0.0405-scale orbiter (Model 16-0). The model's angle of attack was set at 10, 15, and 20 degrees while the sideslip had values of minus 5, 0, and plus 5 degrees. The full scale Qbars that were simulated were 105, 128, 160, and 210 psf. In the hatch jettison mechanism itself, the plunger pressure was varied to achieve horizontal velocities of 3, 5, 7, and 20.1 feet per second model scale, and the plunger location was varied to achieve a variety of rotational velocities. The orbiter model was subjected to 122 runs with 13 different hatches. Of these, 60 were good runs.

  19. Effects of egg size, parental quality and hatch-date on growth and survival of Common Tern Sterna hirundo chicks

    USGS Publications Warehouse

    Arnold, J.M.; Hatch, J.J.; Nisbet, I.C.T.

    2006-01-01

    We examined the relative contributions of egg size, parental quality and hatch-date to growth and survival of second-hatched chicks (those chicks making the greatest contribution to differences in productivity among pairs) by exchanging clutches among nests of Common Terns Sterna hirundo matched for lay-date (range 13 May to 9 June). The mass of a second-laid egg in an exchanged clutch ranged from 17.70 to 23.80 g. Growth and survival were studied during three periods: early (days 0-3), middle (days 3-12) and late (days 12-25). Both egg mass and hatch-date were important predictors of hatchling mass (positive relationships), although there was no seasonal trend in egg mass. During the middle period, hatch-date was a significant predictor of mass gain and survival (inverse relationships). After controlling for hatch-date, other indices of parental quality made only small contributions to chick mass gain and survival. Our results suggest that although breeding early generally leads to greater overall survival of chicks, several important interactions among egg 'quality', parental quality and early laying may affect breeding success under specific conditions. ?? 2006 British Ornithologists' Union.

  20. Effects of pulsed turbidity and vessel traffic on lake herring eggs and larvae

    USGS Publications Warehouse

    Savino, Jacqueline F.; Blouin, Marc A.; Davis, Bruce M.; Hudson, Patrick L.; Todd, Thomas N.; Fleischer, Guy W.

    1994-01-01

    Proposals to extend commercial shipping in the St. Marys River (connecting Lakes Superior and Huron) to include winter months have raised concerns regarding its effect on lake herring (Coregonus artedi). Because lake herring spawn in fall and their eggs overwinter in the river and hatch in spring, their hatching success could be impacted by early opening of the locks in spring. Our laboratory studies showed that under the range of turbidities expected in the river due to vessel traffic, lake herring eggs hatched and larvae fed adequately. Field incubation studies produced about 75% survival and 70% hatching success of lake herring eggs at two of three study sites. Collections in the river throughout the month following ice-out showed that sufficient plankton of appropriate size were available to ensure growth and survival of larval lake herring. We did not detect any negative impacts on the early life stages of lake herring as a result of sedimentation in the laboratory or field. However, detailing the spawning sites of lake herring and defining the normal survival-to-hatch in these areas are necessary before making accurate predictions of the effects of early season vessel traffic on lake herring hatching success.

  1. High- and low-temperature manipulation during late incubation: effects on embryonic development, the hatching process, and metabolism in broilers.

    PubMed

    Willemsen, H; Kamers, B; Dahlke, F; Han, H; Song, Z; Ansari Pirsaraei, Z; Tona, K; Decuypere, E; Everaert, N

    2010-12-01

    Temperatures continuously higher and lower than the standard incubation temperature by 3°C from embryonic d 16 until embryonic d 18.5 result in differential effects on embryonic development, the hatching process, and embryonic metabolism. Embryos in the high-temperature group were forced into a state of malnutrition by the temperature treatment, as reflected by reduced embryo growth and yolk consumption, resulting in a significantly lower chick weight at hatch. In addition, altered air cell and blood gases as well as a retarded hatching process further indicated reduced growth of embryos exposed to higher incubation temperatures during the latter part of incubation. In addition, hatchability was significantly reduced by the high-temperature treatment due to higher embryonic mortality during the treatment period and the hatching process. Levels of blood glucose, lactate, liver glycogen, plasma triglycerides, and nonesterified fatty acids indicated an altered carbohydrate and lipid metabolism for the high-temperature group. Although the hatching process of embryos exposed to lower incubation temperatures was also significantly retarded, their embryonic development and growth were strikingly similar to those of the control group.

  2. Hatching asynchrony vs. foraging efficiency: the response to food availability in specialist vs. generalist tit species

    PubMed Central

    Barrientos, R.; Bueno-Enciso, J.; Sanz, J. J.

    2016-01-01

    Breeding mistiming is increasingly frequent in several ecosystems in the face of current climate change. Species belonging to higher trophic levels must employ mechanisms to reduce it. One of these mechanisms is hatching asynchrony, with the eggs in a clutch hatching over a period of several days. Some authors have suggested it to be adaptive when food is unpredictable. However, these birds can also suffer associated costs. We tested whether a species with higher foraging efficiency avoid hatching asynchrony compared to its sister species. We studied hatching asynchrony and nestling provisioning in relation to food availability in sympatric populations of blue and great tits. For the first time, we show that sister species respond to food availability with different strategies. Blue tit feeding rates readily responded to the abundance of their main prey, and also reduced the impact of nestling size hierarchy on mean nestling weight, consequently increasing fledging rate. Our results suggest that levels of hatching asynchrony seem to be influenced by species-specific life history traits, as generalist foragers rely less on it. They also highlight the importance of multi-species approaches when studying the response of organisms to environmental unpredictability. PMID:27892941

  3. Entrainment of the Circadian Rhythm in Egg Hatching of the Crab Dyspanopeus sayi by Chemical Cues from Ovigerous Females.

    PubMed

    Forward, Richard B; Sanchez, Kevin G; Riley, Paul P

    2016-02-01

    The subtidal crab Dyspanopeus sayi has a circadian rhythm in larval release with a free-running period of 24.1 h. Under constant conditions, eggs hatch primarily in the 4-h interval after the time of sunset. The study tested the new model for entrainment in subtidal crabs, which proposes that the female perceives the environmental cycles and entrains the endogenous rhythm in the embryos. Results verified the model for D. sayi. Hatching by embryos collected from the field when they had not yet developed eye pigments, and were kept in constant conditions attached to their mother, exhibited the circadian hatching rhythm. Attached embryos could also be entrained to a new photoperiod in the laboratory before they developed eye pigments. Further, mature embryos removed from the female hatched rhythmically, indicating that a circadian rhythm resides in the embryos. However, if mature embryos with eye pigments were removed from the female and exposed to a new light-dark cycle, they could not be entrained to the new cycle; rather, they hatched according to the timing of the original light-dark cycle. Nevertheless, detached, mature embryos would entrain to a new light-dark cycle if they were in chemical, but not physical, contact with the female. Thus, the female perceives the light-dark cycle, and uses chemical cues to entrain the circadian rhythm of hatching by the embryos. © 2016 Marine Biological Laboratory.

  4. Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurrence of haploids among larvae produced with cryopreserved sperm.

    PubMed

    Horváth, Akos; Miskolczi, Edit; Mihálffy, Szilvia; Osz, Katalin; Szabó, Krisztián; Urbányi, Béla

    2007-06-01

    Experiments were carried out on the cryopreservation of common carp (Cyprinus carpio) sperm in order to test the suitability of using 1.2 and 5 ml straws and to investigate the ploidy of malformed larvae found among the hatched progeny. In the first set of experiments, the effect of freezing time was investigated on the hatch rate of embryos. The highest hatch rate for 1.2 ml straws was 69+/-16% at the freezing time of 4 min, and 39+/-27% for 5 ml straws at 5 min. In the second set, the effect different egg volumes fertilized with one straw of sperm on the hatch rate and the rate of malformed larvae was investigated. The highest hatch rate with 1.2 ml straws (86+/-12%) was observed when 10 g of eggs were fertilized with one straw, whereas with 5 ml straws the hatch rate was highest (65+/-18%) when 40 g of eggs were fertilized. The highest rate of malformed larvae (15+/-9%) was found in the control, whereas the highest rate of malformed larvae among the groups fertilized with cryopreserved sperm (13+/-7%) was found in the 1x dose group fertilized with 5 ml straw. The chromosome numbers of malformed larvae were investigated and haploids were found among those hatched from eggs fertilized with cryopreserved sperm whereas only diploids were found in the controls.

  5. Organic and inorganic selenium in Aseel chicken diets: Effect on hatching traits.

    PubMed

    Khan, M T; Mahmud, A; Zahoor, I; Javed, K

    2017-05-01

    A study was conducted to evaluate the effect of dietary selenium (Se) sources (organic and inorganic Se at 0.30 ppm and basal diet at 0 ppm level of supplemented Se) on hatching traits in four varieties of Aseel chicken, Lakha, Mushki, Peshawari, and Mianwali. In total, 84 adult molted hens (50 wk old), 21 from each variety, were randomly assigned to 12 treatment groups in a 3 (Se diets) × 4 (Aseel varieties) factorial arrangement under a randomized complete block design. Each treatment was replicated 7 times with individual hens in each. Settable egg, fertility, hatch of fertile eggs, hatchability, A-grade chick, and embryonic mortality parameters were evaluated. The results indicated that the birds fed an organic Se supplemented diet had greater (P < 0.05) settable eggs, fertility, hatch of fertile eggs, hatchability, and A-grade chicks and reduced embryonic mortality than those fed inorganic or no Se. Among varieties, Mushki had lower (P < 0.05) fertility, hatch of fertile eggs, hatchability, and A-grade chicks than rest of three varieties. Interaction of Se sources and varieties indicated that dietary organic Se supplementation improved (P < 0.05) hatch of fertile eggs in Peshawari and Mianwali, whereas hatchability only in Peshawari variety and reduced embryonic mortality in Mianwali. It was concluded that dietary supplementation of organic Se could be used to improve hatching traits as well as reduce embryonic mortality in native Aseel chicken. © 2016 Poultry Science Association Inc.

  6. 40 CFR 63.5830 - What are my options for meeting the standards for pultrusion operations subject to the 60 weight...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... included in the total open area calculation with the exception of access panels, doors, and/or hatches that... than 1.0 inch clearance. (7) The access panels, doors, and/or hatches that are part of the enclosure must close tightly. Damaged access panels, doors, and/or hatches that do not close tightly must be...

  7. Lindsey beside hatch to PMM (Permanent Multipurpose Module)

    NASA Image and Video Library

    2011-03-01

    S133-E-007799 (1 March 2011) --- NASA astronaut Steve Lindsey, STS-133 commander, is pictured at the hatch of the Earth-facing port of the International Space Station’s Unity node while space shuttle Discovery remains docked with the station. On the other side of the hatch door is the newly-installed Permanent Multipurpose Module (PMM). Photo credit: NASA or National Aeronautics and Space Administration

  8. 46 CFR 35.05-15 - Tank vessel security-TB/ALL.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... (b)(2) of this section; or (ii) All cargo tank hatches must be clearly marked in not less than three inch lettering “Danger—Keep Out,” and all hatch covers must be closed and dogged down in such a way that the hatch cannot be opened by the use of bare hands alone. [CGFR 65-50, 30 FR 16704, Dec. 30, 1965...

  9. 46 CFR 35.05-15 - Tank vessel security-TB/ALL.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... (b)(2) of this section; or (ii) All cargo tank hatches must be clearly marked in not less than three inch lettering “Danger—Keep Out,” and all hatch covers must be closed and dogged down in such a way that the hatch cannot be opened by the use of bare hands alone. [CGFR 65-50, 30 FR 16704, Dec. 30, 1965...

  10. Training Practices for Surface Warfare Junior Officers

    DTIC Science & Technology

    2011-12-01

    Bowman Alice M . Crawford William D. Hatch December 2011 THIS PAGE INTENTIONALLY LEFT BLANK NAVAL POSTGRADUATE SCHOOL...Alice M . Crawford Assistant Dean for Faculty Development Graduate School of Business & Public Policy William D. Hatch Lecturer Graduate School...NUMBER DRTE9 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) William R. Bowman, Alice M . Crawford, and William D. Hatch 5d. PROJECT

  11. Effects of transgenic sterilization constructs and their repressor compounds on hatch, developmental rate and early survival of electroporated channel catfish embryos and fry.

    PubMed

    Su, Baofeng; Shang, Mei; Li, Chao; Perera, Dayan A; Pinkert, Carl A; Irwin, Michael H; Peatman, Eric; Grewe, Peter; Patil, Jawahar G; Dunham, Rex A

    2015-04-01

    Channel catfish (Ictalurus punctatus) embryos were electroporated with sterilization constructs targeting primordial germ cell proteins or with buffer. Some embryos then were treated with repressor compounds, cadmium chloride, copper sulfate, sodium chloride or doxycycline, to prevent expression of the transgene constructs. Promoters included channel catfish nanos and vasa, salmon transferrin (TF), modified yeast Saccharomyces cerevisiae copper transport protein (MCTR) and zebrafish racemase (RM). Knock-down systems were the Tet-off (nanos and vasa constructs), MCTR, RM and TF systems. Knock-down genes included shRNAi targeting 5' nanos (N1), 3' nanos (N2) or dead end (DND), or double-stranded nanos RNA (dsRNA) for overexpression of nanos mRNA. These constructs previously were demonstrated to knock down nanos, vasa and dead end, with the repressors having variable success. Exogenous DNA affected percentage hatch (% hatch), as all 14 constructs, except for the TF dsRNA, TF N1 (T), RM DND (C), vasa DND (C), vasa N1 (C) and vasa N2 (C), had lower % hatch than the control electroporated with buffer. The MCTR and RM DND (T) constructs resulted in delayed hatch, and the vasa and nanos constructs had minimal effects on time of hatch (P < 0.05). Cadmium chloride appeared to counteract the slow development caused by the TF constructs in two TF treatments (P < 0.05). The 4 ppt sodium chloride treatment for the RM system decreased % hatch (P < 0.05) and slowed development. In the case of nanos constructs, doxycycline greatly delayed hatch (P < 0.05). Adverse effects of the transgenes and repressors continued for several treatments for the first 6 days after hatch, but only in a few treatments during the next 10 days. Repressors and gene expression impacted the yield of putative transgenic channel catfish fry, and need to be considered and accounted for in the hatchery phase of producing transgenically sterilized catfish fry and their fertile counterparts. This fry output should be considered to ensure that sufficient numbers of transgenic fish are produced for future applications and for defining repressor systems that are the most successful.

  12. Seasonal variations in larval biomass and biochemical composition of brown shrimp, Crangon crangon (Decapoda, Caridea), at hatching

    NASA Astrophysics Data System (ADS)

    Urzúa, Ángel; Anger, Klaus

    2013-06-01

    The "brown shrimp", Crangon crangon (Linnaeus 1758), is a benthic key species in the North Sea ecosystem, supporting an intense commercial fishery. Its reproductive pattern is characterized by a continuous spawning season from mid-winter to early autumn. During this extended period, C. crangon shows significant seasonal variations in egg size and embryonic biomass, which may influence larval quality at hatching. In the present study, we quantified seasonal changes in dry weight (W) and chemical composition (CHN, protein and lipid) of newly hatched larvae of C. crangon. Our data revealed significant variations, with maximum biomass values at the beginning of the hatching season (February-March), a decrease throughout spring (April-May) and a minimum in summer (June-September). While all absolute values of biomass and biochemical constituents per larva showed highly significant differences between months ( P < 0.001), CHN, protein and lipid concentrations (expressed as percentage values of dry weight) showed only marginally significant differences ( P < 0.05). According to generalized additive models (GAM), key variables of embryonic development exerted significant effects on larval condition at hatching: The larval carbon content (C) was positively correlated with embryonic carbon content shortly after egg-laying ( r 2 = 0.60; P < 0.001) and negatively with the average incubation temperature during the period of embryonic development ( r 2 = 0.35; P < 0.001). Additionally, water temperature ( r 2 = 0.57; P < 0.001) and food availability (phytoplankton C; r 2 = 0.39; P < 0.001) at the time of hatching were negatively correlated with larval C content at hatching. In conclusion, "winter larvae" hatching from larger "winter eggs" showed higher initial values of biomass compared to "summer larvae" originating from smaller "summer eggs". This indicates carry-over effects persisting from the embryonic to the larval phase. Since "winter larvae" are more likely exposed to poor nutritional conditions, intraspecific variability in larval biomass at hatching is interpreted as part of an adaptive reproductive strategy compensating for strong seasonality in plankton production and transitory periods of larval food limitation.

  13. STS-89 and Mir 24 crews at the hatch opening

    NASA Image and Video Library

    1998-03-04

    S89-E-5359 (28 Jan 1998) --- This Electronic Still Camera (ESC) image shows cosmonaut Anatoliy Y. Solovyev, Mir-24 commander, peeking through the Docking Module (DM) hatch one last time to bid his astronaut friends farewell, just moments before final hatch closure. The hatch closing brings an end to the eighth Shuttle/Mir joint docking activities. The STS-89 crew, onboard the Space Shuttle Endeavour, dropped off astronaut Andrew S. W. Thomas to replace astronaut David A. Wolf, as cosmonaut guest researcher. Thomas will be the last American astronaut to serve a tour aboard the Russian Mir Space station. This ESC view was taken on January 28, 1998, at 22:30:54 GMT.

  14. Polyribosome formation and poly(A)-containing RNA in embryos of the sand dollar,Dendraster excentricus.

    PubMed

    Spieth, John; Whiteley, Arthur H

    1981-03-01

    The pattern of appearance of ribosomes, newly synthesized mRNA, and poly(A)-containing mRNA in polyribosomes has been examined in sand dollar embryos. From early blastula until shortly before hatching small polyribosomes engaged in histone synthesis predominate. At the time of hatching, when the rate of cell increase is maximal, the proportion of poly(A)-containing RNA in polyribosomes is low. After hatching a new class of large polyribosomes appears and the amount of poly(A)-containing polyribosomal RNA increases. Cordycepin, an inhibitor of RNA adenylylation, prevents the appearance of the large polyribosomes after hatching as well as the increase in poly(A)-containing polyribosomal RNA.

  15. Prenatal sensory experience affects hatching behavior in domestic chicks (Gallus gallus) and Japanese quail chicks (Coturnix coturnix japonica).

    PubMed

    Sleigh, Merry J; Casey, Michael B

    2014-07-01

    Species-typical developmental outcomes result from organismic and environmental constraints and experiences shared by members of a species. We examined the effects of enhanced prenatal sensory experience on hatching behaviors by exposing domestic chicks (n = 95) and Japanese quail (n = 125) to one of four prenatal conditions: enhanced visual stimulation, enhanced auditory stimulation, enhanced auditory and visual stimulation, or no enhanced sensory experience (control condition). In general, across species, control embryos had slower hatching behaviors than all other embryos. Embryos in the auditory condition had faster hatching behaviors than embryos in the visual and control conditions. Auditory-visual condition embryos showed similarities to embryos exposed to either auditory or visual stimulation. These results suggest that prenatal sensory experience can influence hatching behavior of precocial birds, with the type of stimulation being a critical variable. These results also provide further evidence that species-typical outcomes are the result of species-typical prenatal experiences. © 2013 Wiley Periodicals, Inc.

  16. Effects of High Magneto-Gravitational Environment on Silkworm Embryogenesis

    NASA Astrophysics Data System (ADS)

    Tian, Zongcheng; Li, Muwang; Qian, Airong; Xu, Huiyun; Wang, Zhe; Di, Shengmeng; Yang, Pengfei; Hu, Lifang; Ding, Chong; Zhang, Wei; Luo, Mingzhi; Han, Jing; Gao, Xiang; Huang, Yongping; Shang, Peng

    2010-04-01

    The objective of this research was to observe whether silkworm embryos can survive in a high magneto-gravitational environment (HMGE) and what significant phenotype changes can be produced. The hatching rate, hatching time, life span, growth velocity and cocoon weight of silkworm were measured after silkworm embryos were exposed to HMGE (0 g, 12 T; 1 g, 16 T; and 2 g, 12 T) for a period of time. Compared with the control group, 0 g exposure resulted in a lower hatching rate and a shorter life span. Statistically insignificant morphological changes had been observed for larvae growth velocity, incidence of abnormal markings and weight of cocoons. These results suggest that the effect of HMGE on silkworm embryogenesis is not lethal. Bio-effects of silkworm embryogenesis at 0 g in a HMGE were similar with those of space flight. The hatching time, life span and hatching rates of silkworm may be potential phenotype markers related to exposure in a weightless environment.

  17. Recruitment and establishment of the gut microbiome in arctic shorebirds.

    PubMed

    Grond, Kirsten; Lanctot, Richard B; Jumpponen, Ari; Sandercock, Brett K

    2017-12-01

    Gut microbiota play a key role in host health. Mammals acquire gut microbiota during birth, but timing of gut microbial recruitment in birds is unknown. We evaluated whether precocial chicks from three species of arctic-breeding shorebirds acquire gut microbiota before or after hatching, and then documented the rate and compositional dynamics of accumulation of gut microbiota. Contrary to earlier reports of microbial recruitment before hatching in chickens, quantitative PCR and Illumina sequence data indicated negligible microbiota in the guts of shorebird embryos before hatching. Analyses of chick feces indicated an exponential increase in bacterial abundance of guts 0-2 days post-hatch, followed by stabilization. Gut communities were characterized by stochastic recruitment and convergence towards a community dominated by Clostridia and Gammaproteobacteria. We conclude that guts of shorebird chicks are likely void of microbiota prior to hatch, but that stable gut microbiome establishes as early as 3 days of age, probably from environmental inocula. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  18. Convair-240 aircraft modified with shuttle hatch for CES testing

    NASA Technical Reports Server (NTRS)

    1987-01-01

    Shuttle Crew Escape System (CES) hardware includes space shuttle side hatch incorporated into Convair-240 aircraft at Naval Weapons Center, China Lake, California. Closeup shows dummy positioned in the Convair-240 escape hatch. Beginning this month, tests will be conducted here to evaluate a tractor rocket system - one of two escape methods being studied by NASA to provide crew egress capability during Space Shuttle controlled gliding flight.

  19. 49 CFR 231.28 - Box and other house cars with roof hatches built or placed in service after October 1, 1966.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Box and other house cars with roof hatches built... RAILROAD SAFETY APPLIANCE STANDARDS § 231.28 Box and other house cars with roof hatches built or placed in... between treads nineteen (19) inches. (3) Location. One (1) on each end of car not more than eight (8...

  20. 49 CFR 231.28 - Box and other house cars with roof hatches built or placed in service after October 1, 1966.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Box and other house cars with roof hatches built... RAILROAD SAFETY APPLIANCE STANDARDS § 231.28 Box and other house cars with roof hatches built or placed in... between treads nineteen (19) inches. (3) Location. One (1) on each end of car not more than eight (8...

  1. 49 CFR 231.28 - Box and other house cars with roof hatches built or placed in service after October 1, 1966.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Box and other house cars with roof hatches built... RAILROAD SAFETY APPLIANCE STANDARDS § 231.28 Box and other house cars with roof hatches built or placed in... between treads nineteen (19) inches. (3) Location. One (1) on each end of car not more than eight (8...

  2. DETAIL OF OPEN HATCH SHOWING INTERIOR OF MISSILE TUBE AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL OF OPEN HATCH SHOWING INTERIOR OF MISSILE TUBE AND OPEN HATCH AND DOOR ON OPPOSITE SIDE OF TUBE (AT THIRD LEVEL OF MISSILE LAB). VIEW FACING WEST - U.S. Naval Base, Pearl Harbor, Ford Island Polaris Missile Lab & U.S. Fleet Ballistic Missile Submarine Training Center, Between Lexington Boulvevard and the sea plane ramps on the southwest side of Ford Island, Pearl City, Honolulu County, HI

  3. Influence of thermal stimulation during the late phase of incubation on hatching results and post-hatch broiler performance under commercial conditions.

    PubMed

    Elmehdawi, A S; Hall, M A; Skewes, P A; Wicker, D L; Maurice, D V

    2016-12-01

    Two experiments, which differed in breeder age, strain and season, were conducted to study the influence of low-intensity, short-duration thermal stimuli during the late phase of incubation on hatchability and performance. The first experiment conducted in April-June used eggs from Cobb × Ross broiler breeders at 35-41 weeks of age and the second experiment performed in February-April used eggs from Hubbard × Cobb broiler breeders at 49-53 weeks of age. Eggs in the test group had the same physical environment as eggs in the control group except that incubation temperature was increased by 1˚C for 2 h/d above the control group from 18 to 20 d of incubation (DI). The results demonstrated that thermal stimulation of 1˚C for 2 h/d above control incubation temperature during 18-21DI did not have any adverse effects on hatch and post-hatch performance of broilers. In both experiments, treatment did not significantly alter the secondary sex ratio in hatched chickens, but hatch residue showed that the proportion of unhatched male embryos was significantly lower in the test groups than in the control groups. In the first experiment, thermal stimulation improved feed conversion by 1.82% compared with the control.

  4. Fungicides affect Japanese beetle Popillia japonica (Coleoptera: Scarabaeidae) egg hatch, larval survival and detoxification enzymes.

    PubMed

    Obear, Glen R; Adesanya, Adekunle W; Liesch, Patrick J; Williamson, R Chris; Held, David W

    2016-05-01

    Larvae of the Japanese beetle, Popillia japonica (Coleoptera: Scarabaeidae), have a patchy distribution in soils, which complicates detection and management of this insect pest. Managed turf systems are frequently under pest pressure from fungal pathogens, necessitating frequent fungicide applications. It is possible that certain turfgrass fungicides may have lethal or sublethal adverse effects on eggs and larvae of P. japonica that inhabit managed turf systems. In this study, eggs and first-, second- and third-instar larvae were treated with the fungicides chlorothalonil and propiconazole, and survival was compared with that of untreated controls as well as positive controls treated with the insecticide trichlorfon. Chlorothalonil reduced survival of first-instar larvae treated directly and hatched from treated eggs. Propiconazole delayed egg hatch, reduced the proportion of eggs that successfully hatched and reduced survival of first-instar larvae treated directly and hatched from treated eggs. Sublethal doses of the fungicides lowered the activities of certain detoxification enzymes in third-instar grubs. Fungicide applications to turfgrass that coincide with oviposition and egg hatch of white grubs may have sublethal effects. This work is applicable both to high-maintenance turfgrass such as golf courses, where applications of pesticides are more frequent, and to home lawn services, where mixtures of multiple pesticides are commonly used. © 2015 Society of Chemical Industry.

  5. Effect of a photoperiodic green light programme during incubation on embryo development and hatch process.

    PubMed

    Tong, Q; McGonnell, I M; Demmers, T G M; Roulston, N; Bergoug, H; Romanini, C E; Verhelst, R; Guinebretière, M; Eterradossi, N; Berckmans, D; Exadaktylos, V

    2018-04-01

    This study was conducted to evaluate the effect of a 12-h light, 12-h dark (12L : 12D) photoperiod of green light during day 1 to day 18 of incubation time, on embryo growth, hormone concentration and the hatch process. In the test group, monochromatic light was provided by a total of 204 green light-emitting diodes (522 nm) mounted in a frame which was placed above the top tray of eggs to give even spread of illumination. No light-dark cycle was used in the control group. Four batches of eggs (n=300/group per batch) from fertile Ross 308 broiler breeders were used in this experiment. The beak length and crown-rump length of embryos incubated under green light were significantly longer than that of control embryos at day 10 and day 12, respectively (P<0.01). Furthermore, green light-exposed embryos had a longer third toe length compared with control embryos at day 10, day 14 and day 17 (P=0.02). At group level (n=4 batches), light stimulation had no effect on chick weight and quality at take-off, the initiation of hatch and hatch window. However, the individual hatching time of the light exposure focal chicks (n=33) was 3.4 h earlier (P=0.49) than the control focal chicks (n=36) probably due to the change in melatonin rhythm of the light group. The results of this study indicate that green light accelerates embryo development and alters hatch-related hormones (thyroid and corticosterone), which may result in earlier hatching.

  6. Stage selection and restricted oviposition period improves cryopreservation of dipteran embryos.

    PubMed

    Rajamohan, Arun; Rinehart, Joseph P; Leopold, Roger A

    2015-04-01

    Embryos of two dipteran species (Musca domestica and Lucilia sericata) were assessed for an effective sampling time that would result in the highest post-cryopreservation hatch rate, with a primary goal to define species-specific egg collection periods and the effects of manual stage selection on post cryopreservation yield. The effects of the time taken to collect eggs on, (a) the proportion of embryos reaching a specific developmental stage between 17 and 20 h of development, and (b) the post-cryopreservation hatch rate were assessed. Permeabilization treatment applied at any stage of embryonic development did not significantly reduce embryo viability. Eggs collected over longer durations significantly reduced the number of embryos available in a specific developmental stage amenable to cryopreservation. Hatch percentage after cryopreservation of the embryos of M. domestica collected over a 60 min period was 10.7 ± 8.7% compared to 31 ± 5% for the eggs collected for just 15 min. Similarly, percent hatch in L. sericata resulted in 17.0 ± 3.9 and <2% for 15 and 60 min samples, respectively. Significantly higher hatching rates were obtained for cryopreservation after manual selection of specific embryonic developmental stages from the dechorionated samples. Post-cryopreservation hatching rate for stage-selected M. domestica embryos was 86.5 ± 5.5% compared to 33.3 ± 4.5% for embryos staged only by an overall visual confirmation. In the case of L. sericata, the hatching percentage was 79.0 ± 11.1 for stage-selected embryos compared to 17.0 ± 3.9% without individual selection. Published by Elsevier Inc.

  7. Embryonic death is linked to maternal identity in the leatherback turtle (Dermochelys coriacea).

    PubMed

    Rafferty, Anthony R; Santidrián Tomillo, Pilar; Spotila, James R; Paladino, Frank V; Reina, Richard D

    2011-01-01

    Leatherback turtles have an average global hatching success rate of ~50%, lower than other marine turtle species. Embryonic death has been linked to environmental factors such as precipitation and temperature, although, there is still a lot of variability that remains to be explained. We examined how nesting season, the time of nesting each season, the relative position of each clutch laid by each female each season, maternal identity and associated factors such as reproductive experience of the female (new nester versus remigrant) and period of egg retention between clutches (interclutch interval) affected hatching success and stage of embryonic death in failed eggs of leatherback turtles nesting at Playa Grande, Costa Rica. Data were collected during five nesting seasons from 2004/05 to 2008/09. Mean hatching success was 50.4%. Nesting season significantly influenced hatching success in addition to early and late stage embryonic death. Neither clutch position nor nesting time during the season had a significant affect on hatching success or the stage of embryonic death. Some leatherback females consistently produced nests with higher hatching success rates than others. Remigrant females arrived earlier to nest, produced more clutches and had higher rates of hatching success than new nesters. Reproductive experience did not affect stage of death or the duration of the interclutch interval. The length of interclutch interval had a significant affect on the proportion of eggs that failed in each clutch and the developmental stage they died at. Intrinsic factors such as maternal identity are playing a role in affecting embryonic death in the leatherback turtle.

  8. Olive Ridley Sea Turtle Hatching Success as a Function of the Microbial Abundance in Nest Sand at Ostional, Costa Rica

    PubMed Central

    Bézy, Vanessa S.; Valverde, Roldán A.; Plante, Craig J.

    2015-01-01

    Several studies have suggested that significant embryo mortality is caused by microbes, while high microbial loads are generated by the decomposition of eggs broken by later nesting turtles. This occurs commonly when nesting density is high, especially during mass nesting events (arribadas). However, no previous research has directly quantified microbial abundance and the associated effects on sea turtle hatching success at a nesting beach. The aim of this study was to test the hypothesis that the microbial abundance in olive ridley sea turtle nest sand affects the hatching success at Ostional, Costa Rica. We applied experimental treatments to alter the microbial abundance within the sand into which nests were relocated. We monitored temperature, oxygen, and organic matter content throughout the incubation period and quantified the microbial abundance within the nest sand using a quantitative polymerase chain reaction (qPCR) molecular analysis. The most successful treatment in increasing hatching success was the removal and replacement of nest sand. We found a negative correlation between hatching success and fungal abundance (fungal 18S rRNA gene copies g-1 nest sand). Of secondary importance in determining hatching success was the abundance of bacteria (bacterial 16S rRNA gene copies g-1 g-1 nest sand). Our data are consistent with the hypothesis that high microbial activity is responsible for the lower hatching success observed at Ostional beach. Furthermore, the underlying mechanism appears to be the deprivation of oxygen and exposure to higher temperatures resulting from microbial decomposition in the nest. PMID:25714355

  9. Hypoxia impairs embryo development and survival in black bream (Acanthopagrus butcheri).

    PubMed

    Hassell, Kathryn L; Coutin, Patrick C; Nugegoda, Dayanthi

    2008-01-01

    Coastal environments are threatened by the increasing frequency, extent and severity of hypoxic events. Hypoxia affects vast areas around the world and often causes fish kills, reduced abundance, altered distribution, low benthic biomass and declines in fisheries. In Australia, many fisheries are based on sparid fishes and in the southern states black bream (Acanthopagrus butcheri) is important to both the recreational and commercial sectors. This species completes its entire life cycle in estuaries and annual recruitment is highly variable and very likely influenced by environmental conditions during the spawning season. In a laboratory-based experiment, fertilised black bream eggs (embryos) were exposed to five different levels of dissolved oxygen (DO). The DO levels were maintained in small test wells using nitrogen gas in a novel chamber design. Embryo development was assessed over a 2-day period and hatched larvae were observed until Day 2 post-hatch. Significant differences (p<0.05) were observed in embryonic development and survival as a function of DO level. In severely hypoxic conditions (30% saturation) survival to 1 day was reduced and no hatching occurred. In moderately hypoxic conditions (45-55%S), both precocious and delayed hatching was observed and hatch rates were reduced, whilst the number of hatched larvae with deformities increased, resulting in reduced larval lengths. No larvae survived to Day 2 post-hatch when held in hypoxic conditions (<55%S). This study demonstrates the detrimental effect that severe hypoxia can have on the early development of black bream which could result in reduced recruitment and lowered abundance. Other species that share similar early life histories may also be at risk.

  10. The effects of increased constant incubation temperature and cumulative acute heat shock exposures on morphology and survival of Lake Whitefish (Coregonus clupeaformis) embryos.

    PubMed

    Lee, Abigail H; Eme, John; Mueller, Casey A; Manzon, Richard G; Somers, Christopher M; Boreham, Douglas R; Wilson, Joanna Y

    2016-04-01

    Increasing incubation temperatures, caused by global climate change or thermal effluent from industrial processes, may influence embryonic development of fish. This study investigates the cumulative effects of increased incubation temperature and repeated heat shocks on developing Lake Whitefish (Coregonus clupeaformis) embryos. We studied the effects of three constant incubation temperatures (2°C, 5°C or 8°C water) and weekly, 1-h heat shocks (+3°C) on hatching time, survival and morphology of embryos, as these endpoints may be particularly susceptible to temperature changes. The constant temperatures represent the predicted magnitude of elevated water temperatures from climate change and industrial thermal plumes. Time to the pre-hatch stage decreased as constant incubation temperature increased (148d at 2°C, 92d at 5°C, 50d at 8°C), but weekly heat shocks did not affect time to hatch. Mean survival rates and embryo morphometrics were compared at specific developmental time-points (blastopore, eyed, fin flutter and pre-hatch) across all treatments. Constant incubation temperatures or +3°C heat-shock exposures did not significantly alter cumulative survival percentage (~50% cumulative survival to pre-hatch stage). Constant warm incubation temperatures did result in differences in morphology in pre-hatch stage embryos. 8°C and 5°C embryos were significantly smaller and had larger yolks than 2°C embryos, but heat-shocked embryos did not differ from their respective constant temperature treatment groups. Elevated incubation temperatures may adversely alter Lake Whitefish embryo size at hatch, but weekly 1-h heat shocks did not affect size or survival at hatch. These results suggest that intermittent bouts of warm water effluent (e.g., variable industrial emissions) are less likely to negatively affect Lake Whitefish embryonic development than warmer constant incubation temperatures that may occur due to climate change. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Influence of hatch time and access to feed on intramuscular adipose tissue deposition in broilers.

    PubMed

    Powell, D J; Velleman, S G; Cowieson, A J; Singh, M; Muir, W I

    2016-06-01

    The effect of hatch time and subsequent access to feed on intramuscular adipose tissue deposition was studied in the pectoralis major muscle of male Ross 308 broiler chickens. Based on their hatch time chicks were classified as early (EH), midterm (MH), or late (LH) hatchers, with an average incubation duration of 497.7 h for EH, 508.8 h for MH, and 514.5 h for LH birds. Chicks were provided access to feed either immediately at hatch, or 24 h after the conclusion of the hatch window. Expression of the adipogenic regulatory genes peroxisome proliferator-activated receptor gamma (PPARγ), and stearoyl-CoA desaturase (SCD), were measured at the time of hatch, and zero, one, 4, 7, 28, and 40 d. Intramuscular adipocyte cell width and visualization of adipose tissue deposition was observed at 28 and 40 d. Expression of PPARγ was increased in the pectoralis major of LH birds at the time of hatch, zero, and one d. The expression of PPARγ at one and 7 d, and SCD at 7 d were increased in all birds that received delayed access to feed. At 28 d, adipocyte cell width was increased in LH birds with delayed access to feed, compared to EH and MH birds with delayed access to feed and LH birds with immediate access to feed. At 40 d, adipocyte cell width was increased in all birds that received delayed access to feed. Also at 40 d, there was a trend (P = 0.078) for more extensive intramuscular adipose tissue deposition in LH than EH birds, and in birds with delayed access to feed (P = 0.075). These data indicate delayed access to feed increases intramuscular adipose tissue deposition in the pectoralis major muscle, and suggest that hatch time influences this regulation. © 2016 Poultry Science Association Inc.

  12. Maternal health status correlates with nest success of leatherback sea turtles (Dermochelys coriacea) from Florida.

    PubMed

    Perrault, Justin R; Miller, Debra L; Eads, Erica; Johnson, Chris; Merrill, Anita; Thompson, Larry J; Wyneken, Jeanette

    2012-01-01

    Of the seven sea turtle species, the critically endangered leatherback sea turtle (Dermochelys coriacea) exhibits the lowest and most variable nest success (i.e., hatching success and emergence success) for reasons that remain largely unknown. In an attempt to identify or rule out causes of low reproductive success in this species, we established the largest sample size (n = 60-70 for most values) of baseline blood parameters (protein electrophoresis, hematology, plasma biochemistry) for this species to date. Hematologic, protein electrophoretic and biochemical values are important tools that can provide information regarding the physiological condition of an individual and population health as a whole. It has been proposed that the health of nesting individuals affects their reproductive output. In order to establish correlations with low reproductive success in leatherback sea turtles from Florida, we compared maternal health indices to hatching success and emergence success of their nests. As expected, hatching success (median = 57.4%) and emergence success (median = 49.1%) in Floridian leatherbacks were low during the study period (2007-2008 nesting seasons), a trend common in most nesting leatherback populations (average global hatching success = ∼50%). One protein electrophoretic value (gamma globulin protein) and one hematologic value (red blood cell count) significantly correlated with hatching success and emergence success. Several maternal biochemical parameters correlated with hatching success and/or emergence success including alkaline phosphatase activity, blood urea nitrogen, calcium, calcium:phosphorus ratio, carbon dioxide, cholesterol, creatinine, and phosphorus. Our results suggest that in leatherbacks, physiological parameters correlate with hatching success and emergence success of their nests. We conclude that long-term and comparative studies are needed to determine if certain individuals produce nests with lower hatching success and emergence success than others, and if those individuals with evidence of chronic suboptimal health have lower reproductive success.

  13. Geographical and temporal variation in environmental conditions affects nestling growth but not immune function in a year-round breeding equatorial lark.

    PubMed

    Ndithia, Henry K; Bakari, Samuel N; Matson, Kevin D; Muchai, Muchane; Tieleman, B Irene

    2017-01-01

    Variation in growth and immune function within and among populations is often associated with specific environmental conditions. We compared growth and immune function in nestlings of year-round breeding equatorial Red - capped Lark Calandrella cinerea from South Kinangop, North Kinangop and Kedong (Kenya), three locations that are geographically close but climatically distinct. In addition, we studied growth and immune function of lark nestlings as a function of year - round variation in breeding intensity and rain within one location. We monitored mass, wing, and tarsus at hatching (day 1) and at 4, 7, and 10 days post - hatch, and we quantified four indices of immune function (haptoglobin, agglutination, lysis and nitric oxide) using blood samples collected on day 10. Nestling body mass and size at hatching, which presumably reflect the resources that females allocated to their eggs, were lowest in the most arid location, Kedong. Contrary to our predictions, nestlings in Kedong grew faster than nestlings in the two other cooler and wetter locations of South and North Kinangop. During periods of peak reproduction within Kedong, nestlings were heavier at hatching, but they did not grow faster over the first 10 days post - hatch. In contrast, rainfall, which did not relate to timing of breeding, had no effect on hatching mass, but more rain did coincide with faster growth post - hatch. Finally, we found no significant differences in nestling immune function, neither among locations nor with the year - round variation within Kedong. Based on these results, we hypothesize that female body condition determines nestling mass and size at hatching, but other independent environmental conditions subsequently shape nestling growth. Overall, our results suggest that environmental conditions related to food availability for nestlings are relatively unimportant to the timing of breeding in equatorial regions, while these same conditions do have consequences for nestling size and growth.

  14. Survey of Hatching Spines of Bee Larvae Including Those of Apis mellifera (Hymenoptera: Apoidea).

    PubMed

    Rozen, Jerome G; Shepard Smith, Corey; Cane, James H

    2017-07-01

    This article explores the occurrence of hatching spines among bee taxa and how these structures enable a larva on hatching to extricate itself from the egg chorion. These spines, arranged in a linear sequence along the sides of the first instar just dorsal to the spiracles, have been observed and recorded in certain groups of solitary and cleptoparasitic bee taxa. After eclosion, the first instar remains loosely covered by the egg chorion. The fact that this form of eclosion has been detected in five families (Table 1 identifies four of the families. The fifth family is the Andrenidae for which the presence of hatching spines in the Oxaeinae will soon be announced.) of bees invites speculation as to whether it is a fundamental characteristic of bees, or at least of solitary and some cleptoparasitic bees. The wide occurrence of these spines has prompted the authors to explore and discover their presence in the highly eusocial Apis mellifera L. Hatching spines were indeed discovered on first instar A. mellifera. The honey bee hatching process appears to differ in that the spines are displayed somewhat differently though still along the sides of the body, and the chorion, instead of splitting along the sides of the elongate egg, seems to quickly disintegrate from the emerging first instar in association with the nearly simultaneous removal of the serosa that covers and separates the first instar from the chorion. Unexpected observations of spherical bodies of various sizes perhaps containing dissolving enzymes being discharged from spiracular openings during hatching may shed future light on the process of how A. mellifera effects chorion removal during eclosion. Whereas hatching spines occur among many groups of bees, they appear to be entirely absent in the Nomadinae and parasitic Apinae, an indication of a different eclosion process. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America.

  15. Maternal Health Status Correlates with Nest Success of Leatherback Sea Turtles (Dermochelys coriacea) from Florida

    PubMed Central

    Perrault, Justin R.; Miller, Debra L.; Eads, Erica; Johnson, Chris; Merrill, Anita; Thompson, Larry J.; Wyneken, Jeanette

    2012-01-01

    Of the seven sea turtle species, the critically endangered leatherback sea turtle (Dermochelys coriacea) exhibits the lowest and most variable nest success (i.e., hatching success and emergence success) for reasons that remain largely unknown. In an attempt to identify or rule out causes of low reproductive success in this species, we established the largest sample size (n = 60–70 for most values) of baseline blood parameters (protein electrophoresis, hematology, plasma biochemistry) for this species to date. Hematologic, protein electrophoretic and biochemical values are important tools that can provide information regarding the physiological condition of an individual and population health as a whole. It has been proposed that the health of nesting individuals affects their reproductive output. In order to establish correlations with low reproductive success in leatherback sea turtles from Florida, we compared maternal health indices to hatching success and emergence success of their nests. As expected, hatching success (median = 57.4%) and emergence success (median = 49.1%) in Floridian leatherbacks were low during the study period (2007–2008 nesting seasons), a trend common in most nesting leatherback populations (average global hatching success = ∼50%). One protein electrophoretic value (gamma globulin protein) and one hematologic value (red blood cell count) significantly correlated with hatching success and emergence success. Several maternal biochemical parameters correlated with hatching success and/or emergence success including alkaline phosphatase activity, blood urea nitrogen, calcium, calcium∶phosphorus ratio, carbon dioxide, cholesterol, creatinine, and phosphorus. Our results suggest that in leatherbacks, physiological parameters correlate with hatching success and emergence success of their nests. We conclude that long-term and comparative studies are needed to determine if certain individuals produce nests with lower hatching success and emergence success than others, and if those individuals with evidence of chronic suboptimal health have lower reproductive success. PMID:22359635

  16. Generation of Collapsed Cross Sections for Hatch 1 Cycles 1-3

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ade, Brian J

    2012-11-01

    Under NRC JCN V6361, Oak Ridge National Laboratory (ORNL) was tasked to develop and run SCALE/TRITON models for generation of collapsed few-group cross sections and to convert the cross sections to PMAXS format using the GENPMAXS conversion utility for use in PARCS/PATHS simulations of Hatch Unit 1, cycles 1-3. This letter report documents the final models used to produce the Hatch collapsed cross sections.

  17. In ovo feeding of creatine pyruvate alters energy reserves, satellite cell mitotic activity and myogenic gene expression of breast muscle in embryos and neonatal broilers.

    PubMed

    Zhao, M M; Gao, T; Zhang, L; Li, J L; Lv, P A; Yu, L L; Gao, F; Zhou, G H

    2017-09-01

    We investigated the effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on energy reserves, satellite cell mitotic activity (SCMA) and myogenic gene expression in breast muscle of embryos and neonatal broilers. A total of 960 eggs were randomly allocated into three treatments: 1) non-injected control group, 2) saline group injected with 0.6 mL of physiological saline (0.75%), and 3) CrPyr group injected with 0.6 mL of physiological saline (0.75%) containing 12 mg CrPyr/egg at 17.5 d of incubation. After hatching, a total of 120 male chicks were randomly assigned to each treatment group, with eight replicate sets per group. Selected chicks had body BW close to the average of their pooled group. Our results showed that the total and relative breast muscle weights of broilers subjected to CrPyr treatment were higher than those in the control and saline groups on 19 d of incubation (19 E), the day of hatch, 3 and 7 d post-hatch (P < 0.05). The myofiber diameter and cross-sectional area of individuals in the CrPyr group were higher than those in other treatments on 3 and 7 d post-hatch (P < 0.05). Moreover, IOF of CrPyr increased (P < 0.05) creatine concentrations on 19 E, the day of hatch and 3 d post-hatch, the same treatment increased phosphocreatine concentrations on 19 E. Broilers in the CrPyr group showed higher expression of myogenic differentiation 1 (MyoD) (P < 0.05), myogenin and paired box 7 (Pax7), as well as higher index of SCMA on 3 d post-hatch. However, myostatin mRNA expression in CrPyr-treated broilers was down-regulated on 3 d post-hatch (P < 0.05). These results indicated that IOF of CrPyr increased energy reserves of embryos and SCMA of broilers on 3 d post-hatch, which led to enhanced muscle growth in the late embryos and neonatal broilers. Additionally, IOF of CrPyr increased the activity of satellite cells possibly through up-regulating MyoD, myogenin, and Pax7 mRNA expression and down-regulating myostatin mRNA expression. © 2017 Poultry Science Association Inc.

  18. Growth rates of young-of-year shovelnose sturgeon in the Upper Missouri River

    USGS Publications Warehouse

    Braaten, P. J.; Fuller, D.B.

    2007-01-01

    Information on growth during the larval and young-of-year life stages in natural river environments is generally lacking for most sturgeon species. In this study, methods for estimating ages and quantifying growth were developed for field-sampled larval and young-of-year shovelnose sturgeon Scaphirhynchus platorynchus in the upper Missouri River. First, growth was assessed by partitioning samples of young-of-year shovelnose sturgeon into cohorts, and regressing weekly increases in cohort mean length on sampling date. This method quantified relative growth because ages of the cohorts were unknown. Cohort increases in mean length among sampling dates were positively related (P < 0.05, r2 > 0.59 for all cohorts) to sampling date, and yielded growth rate estimates of 0.80–2.95 mm day−1 (2003) and 0.44–2.28 mm day−1 (2004). Highest growth rates occurred in the largest (and earliest spawned) cohorts. Second, a method was developed to estimate cohort hatch dates, thus age on date of sampling could be determined. This method included quantification of post-hatch length increases as a function of water temperature (growth capacity; mm per thermal unit, mm TU−1), and summation of mean daily water temperatures to achieve the required number of thermal units that corresponded to post-hatch lengths of shovelnose sturgeon on sampling dates. For six of seven cohorts of shovelnose sturgeon analyzed, linear growth models (r2 ≥ 0.65, P < 0.0001) or Gompertz growth models (r2 ≥ 0.83, P < 0.0001) quantified length-at-age from hatch through 55 days post-hatch (98–100 mm). Comparisons of length-at-age derived from the growth models indicated that length-at-age was greater for the earlier-hatched cohorts than later-hatched cohorts. Estimated hatch dates for different cohorts were corroborated based on the dates that newly-hatched larval shovelnose sturgeon were sampled in the drift. These results provide the first quantification of growth dynamics for field-sampled age-0 shovelnose sturgeon in a natural river environment, and provide an accurate method for estimating age of wild-caught individuals. Methods of age determination used in this study have applications to sturgeons in other regions, but require additional testing and validation.

  19. Effect of antithyroid drug on chick embryos during the last week of development: delayed hatching and decreased cerebellar acetylcholinesterase activity.

    PubMed

    Haba, Gen; Nishigori, Hidekazu; Tezuka, Yu; Kagami, Keisuke; Sugiyama, Toru; Nishigori, Hideo

    2011-11-01

    Hypothyroid state during embryogenesis disturbs normal growth and brain development, influencing later life. To evaluate the harmful consequences of the state during embryogenesis using an animal model, we inhibited thyroid hormone biosynthesis in chick embryos by using methimazole (MMI). Typically, embryos were treated with MMI (20 µmol/egg) on day 14, and examined on specific days.  Of the control embryos, 94% hatched on day 21, whereas 0% and 60% of MMI-treated embryos hatched on days 21 and 24, respectively. MMI retarded the rates of bodyweight gain as well as liver and heart development, and delayed hatching. However, the external differences in appearance and differences in the weights of the newly hatched control chicks on day 21 and the MMI-treated chicks on day 24 were less obvious. Embryos treated with MMI exhibited increased mass in their brain parts on day 24. Most notably, the treatment resulted in a 1.35-fold increase in cerebellum weight compared to that of the untreated animals. Acetylcholinesterase activity in the cerebellum on the day of hatching decreased significantly to 0.85-fold that of the untreated controls. Thyroid hormone receptor β mRNA was detected from day 12 and dramatically expressed from day 19 to the day of hatching. The 'fertilized hen's egg-chick embryo-chick system' is an appropriate animal model for investigating the hypothyroid state during embryogenesis. Decreased cerebellar acetylcholinesterase activity after MMI treatment was assumed to relate to a mechanism of motor and cognitive deficits in congenital hypothyroidism. © 2011 The Authors. Journal of Obstetrics and Gynaecology Research © 2011 Japan Society of Obstetrics and Gynecology.

  20. Lowering prolactin reduces post-hatch parental care in male and female zebra finches (Taeniopygia guttata).

    PubMed

    Smiley, Kristina O; Adkins-Regan, Elizabeth

    2018-02-01

    Parental care is a widespread phenomenon observed in many diverse taxa. Neuroendocrine systems have long been thought to play an important role in stimulating the onset of parental behavior. In most birds with altricial young, circulating prolactin (PRL) levels are low during non-breeding times and significantly increase during late incubation and early post-hatch chick care. Because of this pattern, PRL has been suggested to be involved in the initiation of parental care in birds, but rarely has this hypothesis been causally tested. To begin testing the hypothesis, we inhibited the release of endogenous PRL with bromocriptine (BR) on the 3days prior to hatching in incubating parents and the first 2days of post-hatch care, when PRL was found to be highest in zebra finches. Nest temperatures were recorded during all 5days and parental behavior was recorded on days 1-2 post-hatch. In addition to hormonal systems, reproductive experience may also influence parental care; therefore, we tested age-matched inexperienced and experienced pairs in each group. BR either eliminated or drastically reduced chick brooding and feeding behavior, resulting in decreased nest temperatures on days 1 and 2 post-hatch. Experienced control birds fed chicks more than inexperienced birds and control females fed more than males. Chick feeding behavior was positively correlated in control male-female pairs, but not in BR pairs. This is one of the few causal studies to demonstrate that PRL is necessary for post-hatch care in a biparental songbird, and is the first to show this effect in zebra finches. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Behavior differentiation between wild Japanese quail, domestic quail, and their first filial generation.

    PubMed

    Chang, G B; Liu, X P; Chang, H; Chen, G H; Zhao, W M; Ji, D J; Chen, R; Qin, Y R; Shi, X K; Hu, G S

    2009-06-01

    The number of wild quail has dramatically reduced in China and reached a state of endangerment with the deterioration of the environment in recent years. In this study, we examined the ecological behaviors of quails in the cage to determine the differentiation level between wild Japanese quail and domestic quail, to detect the relationship between quail behavior and evolutionary differentiation and to analyze the possibility of restoring effective size of wild population. With the on-the-spot observations and measurements, the behaviors of 3 categories of quail, namely wild Japanese quail from the Weishan Lake area in China, domestic quail, and their first filial generation (F(1)) were studied. Domestic quail differed from wild Japanese quail in morphological pattern and ecological behaviors, including some indexes of figure type and egg, vocalization, aggression and fighting, and mating, but wild Japanese quail and domestic quail could succeed in mating and reproducing fertile hybrid offspring. There were significant differences between domestic quail and wild Japanese quail in reproductive traits, involved mating times, fertility rate, hatching rate, and hatching rate of fertilized eggs (P < 0.05). The first filial generation presented significant difference from the wild Japanese quail in vocalization, aggression and fighting, mating, hatching rate, hatching rate of fertilized eggs, and some egg indexes (P < 0.05) and significantly differ from the domestic quail in vocalization, hatching rate, and hatching rate of fertilized eggs (P < 0.05). Evolutionary differentiation between wild quail and domestic quail was still at a relatively low level because no reproductive isolation existed. The advantages of the F(1) hybrids in reproductive capacity, fertilization, and hatching recommend that releasing hybrids instead of domestic quails to the wild would be a more effective way to restore the effective size of wild quail population if necessary.

  2. Development of the cerebellum in the platypus (Ornithorhynchus anatinus) and short-beaked echidna (Tachyglossus aculeatus).

    PubMed

    Ashwell, Ken W S

    2012-01-01

    The monotremes are a unique group of mammals whose young are incubated in a leathery-shelled egg and fed with milk from teatless areolae after hatching. As soon as they hatch, monotreme young must be able to maneuver around the nest or maternal pouch to locate the areolae and stimulate milk ejection. In the present study, the embryological collections at the Museum für Naturkunde, Berlin, have been used to follow the development of the monotreme cerebellum through incubation and lactational phases, to determine whether cerebellar circuitry is able to contribute to the coordination of locomotion in the monotreme hatchling, and to correlate cerebellar development with behavioral maturation. The structure of the developing monotreme cerebellum and the arrangement of transitory neuronal populations are similar to those reported for fetal and neonatal eutherians, but the time course of the key events of later cerebellar development is spread over a much longer period. Expansion of the rostral rhombic lip and formation of the nuclear and cortical transitory zones occurs by the time of hatching, but it is not until after the end of the first post-hatching week that deep cerebellar neurons begin to settle in their definitive positions and the Purkinje cell layer can be distinguished. Granule cell formation is also prolonged over many post-hatching months and the external granular layer persists for more than 20 weeks after hatching. The findings indicate that cerebellar circuitry is unlikely to contribute to the coordination of movements in the monotreme peri-hatching period. Those activities are most likely controlled by the spinal cord and medullary reticular formation circuitry. Copyright © 2012 S. Karger AG, Basel.

  3. Effect of cryopreservation on the pre-hatching behavior in the Mexican fruit fly Anastrepha ludens Loew (Diptera, Tephritidae).

    PubMed

    Rajamohan, Arun; Rinehart, Joseph P; Leopold, Roger A

    2018-02-01

    In a sampling of untreated embryos of the economically important fruit pest species, Anastrepha ludens, the cumulative hatch percentage in the lab was noted to be ∼85%. Approximately 70% of the larvae had eclosed through the posterior pole of the egg. This process is effected by the act of Pole Reversal (PR) of the fully developed pre-hatch larva from the wider anterior to the narrower posterior pole of the egg. Investigation of the effects of cryopreservation and various pretreatments prior to cryostorage on the PR behavior was prompted by the observation of significantly lower proportion of cryopreserved embryos exhibiting the PR behavior. Pretreatments (dechorionation and permeabilization) followed by vitrification resulted in delayed hatching, reflecting a slower embryonic development rate of ∼10 h. A smaller proportion of the treated embryos either eclosed from the anterior end of the egg or did not eclose at all despite complete development and prehatch gnawing activity. In the untreated controls, 24.0% of the embryos eclosed from the anterior pole. After permeabilization and cryopreservation, 83% and 55% (adjusted hatch) of the embryos were noted to hatch this way, respectively. An analysis of the hatch count after the treatments shows that factors contributing to the embryos' inability to properly invert polarity is not solely due to cryopreservation but also due to the pretreatment procedures including dechorionation and permeabilization. In fact, the permeabilization pre-treatment contributed the highest to this phenomenon lending support to the view that chemical toxicity rather than physical effects of cryopreservation play a major role in post-cryopreservation effects. Published by Elsevier Inc.

  4. Intraspecific priority effects modify compensatory responses to changes in hatching phenology in an amphibian.

    PubMed

    Murillo-Rincón, Andrea P; Kolter, Nora A; Laurila, Anssi; Orizaola, Germán

    2017-01-01

    In seasonal environments, modifications in the phenology of life-history events can alter the strength of time constraints experienced by organisms. Offspring can compensate for a change in timing of hatching by modifying their growth and development trajectories. However, intra- and interspecific interactions may affect these compensatory responses, in particular if differences in phenology between cohorts lead to significant priority effects (i.e. the competitive advantage that early-hatching individuals have over late-hatching ones). Here, we conducted a factorial experiment to determine whether intraspecific priority effects can alter compensatory phenotypic responses to hatching delay in a synchronic breeder by rearing moor frog (Rana arvalis) tadpoles in different combinations of phenological delay and food abundance. Tadpoles compensated for the hatching delay by speeding up their development, but only when reared in groups of individuals with identical hatching phenology. In mixed phenology groups, strong competitive effects by non-delayed tadpoles prevented the compensatory responses and delayed larvae metamorphosed later than in single phenology treatments. Non-delayed individuals gained advantage from developing with delayed larvae by increasing their developmental and growth rates as compared to single phenology groups. Food shortage prolonged larval period and reduced mass at metamorphosis in all treatments, but it did not prevent compensatory developmental responses in larvae reared in single phenology groups. This study demonstrates that strong intraspecific priority effects can constrain the compensatory growth and developmental responses to phenological change, and that priority effects can be an important factor explaining the maintenance of synchronic life histories (i.e. explosive breeding) in seasonal environments. © 2016 The Authors. Journal of Animal Ecology © 2016 British Ecological Society.

  5. Density dependence and phenological mismatch: consequences for growth and survival of sub-arctic nesting Canada Geese

    USGS Publications Warehouse

    Brook, Rodney W.; Leafloor, James O.; Douglas, David C.; Abraham, Kenneth F.

    2015-01-01

    The extent to which species are plastic in the timing of their reproductive events relative to phenology suggests how change might affect their demography. An ecological mismatch between the timing of hatch for avian species and the peak availability in quality and quantity of forage for rapidly growing offspring might ultimately affect recruitment to the breeding population unless individuals can adjust the timing of breeding to adapt to changing phenology. We evaluated effects of goose density, hatch timing relative to forage plant phenology, and weather indices on annual growth of pre-fledging Canada geese (Branta canadensis) from 1993-2010 at Akimiski Island, Nunavut. We found effects of both density and hatch timing relative to forage plant phenology; the earlier that eggs hatched relative to forage plant phenology, the larger the mean gosling size near fledging. Goslings were smallest in years when hatch was latest relative to forage plant phenology, and when local abundance of breeding adults was highest. We found no evidence for a trend in relative hatch timing, but it was apparent that in early springs, Canada geese tended to hatch later relative to vegetation phenology, suggesting that geese were not always able to adjust the timing of nesting as rapidly as vegetation phenology was advanced. Analyses using forage biomass information revealed a positive relationship between gosling size and per capita biomass availability, suggesting a causal mechanism for the density effect. The effects of weather parameters explained additional variation in mean annual gosling size, although total June and July rainfall had a small additive effect on gosling size. Modelling of annual first year survival probability using mean annual gosling size as an annual covariate revealed a positive relationship, suggesting that reduced gosling growth negatively impacts recruitment.

  6. Social Support, Depression, Self-Esteem, and Coping Among LGBTQ Adolescents Participating in Hatch Youth.

    PubMed

    Wilkerson, J Michael; Schick, Vanessa R; Romijnders, Kim A; Bauldry, Jessica; Butame, Seyram A

    2017-05-01

    Evidence-based interventions that increase social support have the potential to improve the health of lesbian, gay, bisexual, transgender, and queer (LGBTQ) youth. Hatch Youth is a group-level intervention that provides services four nights a week to LGBTQ youth between 13 and 20 years of age. Each Hatch Youth meeting is organized into three 1-hour sections: unstructured social time, consciousness-raising (education), and a youth-led peer support group. Youth attending a Hatch Youth meeting between March and June 2014 (N = 108) completed a cross-sectional survey. Covariate adjusted regression models were used to examine the association between attendance, perceived social support, depressive symptomology, self-esteem, and coping ability. Compared to those who attended Hatch Youth for less than 1 month, participants who attended 1 to 6 months or more than 6 months reported higher social support (β 1-6mo. = 0.57 [0.07, 1.07]; β 6+mo. = 0.44, 95% confidence interval [CI; 0.14, 0.75], respectively). Increased social support was associated with decreased depressive symptomology (β = -4.84, 95% CI [-6.56, -3.12]), increased self-esteem (β = 0.72, 95% CI [0.38, 1.06]), and improved coping ability (β = 1.00, 95% CI [0.66, 1.35]). Hatch Youth is a promising intervention that has the potential to improve the mental health and reduce risk behavior of LGBTQ youth.

  7. Dietary lufenuron reduces egg hatch and influences protein expression in the fruit fly Bactrocera latifrons (Hendel).

    PubMed

    Chang, Chiou Ling; Geib, Scott; Cho, Il Kyu; Li, Qing X; Stanley, David

    2014-08-01

    Lufenuron (LFN), a chitin synthase inhibitor, impacts the fertility of Ceratitis capitata, Bactrocera dorsalis, B. cucurbitae, and B. latifrons. We posed the hypothesis that LFN curtails egg hatch in the solanaceous fruit fly, B. latifrons. In this study, newly emerged virgin adults were sexed and fed for 12 days with varying concentrations of LFN-laced agar diets until sexual maturation. Eggs were collected from 12-d-old adults and the egg hatch was assessed. Egg hatch decreased in adults reared on LFN-treated diets. LFN-treated media did not influence fertility after one gender was reared on experimental and the other on control media before mating. Exposure to LFN-treated medium after mating led to reduced egg hatch. We infer that LFN is not a permanent sterilant, and reduced egg hatch depends on continuous exposure to dietary LFN after mating. Proteomic analysis identified two differentially expressed proteins, a pheromone binding protein and a chitin binding protein, between adults maintained on LFN-treated and control diets. Expression of two genes encoding chitin synthase 2, and chitin binding protein, was altered in adults exposed to dietary LFN. LFN treatments also led to increased expression of two odorant binding proteins one in females and one in males. We surmise these data support our hypothesis and provide insight into LFN actions. © 2014 Wiley Periodicals, Inc.

  8. Ontogeny of the stress response in chinook salmon, Oncorhynchus tshawytscha

    USGS Publications Warehouse

    Feist, G.; Schreck, C.B.

    2001-01-01

    Whole body concentrations of cortisol were determined via radioimmunoassay in chinook salmon, Onchorynchus tshawytscha, during early development in both stressed and non-stressed fish to determine when the corticosteroidogenic stress response first appeared. Progeny from both pooled and individual females were examined to determine if differences existed in offspring from different females. Levels of cortisol were low in eyed eggs, increased at hatch, decreased 2 weeks later and then remained constant thereafter. Differences in cortisol between stressed and control fish were found 1 week after hatch and persisted for the remainder of the study. The magnitude of the stress response, or relative amount of cortisol produced, generally increased from the time when it was first detected, but a decrease in the ability to elicit cortisol was seen 4 weeks after hatching. Cortisol content of separate progeny from two individual females showed a similar pattern to that seen in pooled eggs. Our results indicate that chinook salmon are capable of producing cortisol following a stressful event approximately 1 week after the time of hatching. The decrease in endogenous cortisol content seen 2 weeks after hatching, and the decrease in the magnitude of the stress response seen 4 weeks after hatching may be comparable to developmental events documented in mammals where corticosteroid synthesis is inhibited to neutralize possible detrimental effects of these hormones during critical periods of development.

  9. Hatching success and predation of Bog Turtle (Glyptemys muhlenbergii) eggs in New Jersey and Pennsylvania

    USGS Publications Warehouse

    Zappalorti, Robert T.; Tutterow, Annalee M.; Pittman, Shannon E.; Lovich, Jeffrey E.

    2017-01-01

    Nest-site selection by most turtles affects the survival of females and their offspring. Although bog turtles (Glyptemys muhlenbergii) do not typically leave their wetlands for nesting, nest-site selection can impact hatching success and hatchling survival. Between 1974 and 2012, we monitored the fates of 258 bog turtle eggs incubated in the field and 91 eggs incubated under laboratory conditions from 11 different bogs, fens, or wetland complexes in New Jersey and Pennsylvania. Laboratory-incubated eggs exhibited the greatest hatching success (81%), but we did not detect a significant difference in hatching success between nests protected with predator excluder cages (43%) and unprotected nests (33%). However, we found significantly lower predation rates in protected nests, suggesting that while predator excluder cages successfully reduced predation, other environmental factors persisted to reduce egg survival in the field. Natural hatching success was potentially reduced by poor weather conditions, which may have resulted in embryo developmental problems, dehydration, or embryos drowning in the egg. Our results suggest that egg depredation, coupled with embryo developmental problems and infertility, are limiting factors to hatching success in our study populations. Using predator excluder cages to protect bog turtle eggs in the field, or incubating eggs in the laboratory and releasing hatchlings at original nesting areas, may be an effective conservation tool for recovering populations of this federally threatened species.

  10. Aberrant behavior of mouse embryo development after blastomere biopsy as observed through time-lapse cinematography.

    PubMed

    Ugajin, Tomohisa; Terada, Yukihiro; Hasegawa, Hisataka; Velayo, Clarissa L; Nabeshima, Hiroshi; Yaegashi, Nobuo

    2010-05-15

    To analyze whether blastomere biopsy affects early embryonal growth as observed through time-lapse cinematography. Comparative prospective study between embryos in which a blastomere was removed and embryos in which a blastomere was not removed. An experimental laboratory of the university. We calculated the time between blastocele formation and the end of hatching, the time between the start and end of hatching, the number of contractions and expansions between blastocyst formation and the end of hatching, and the maximum diameter of the expanded blastocyst. In blastomere removal embryos, compaction began at the six-cell stage instead of at the eight-cell stage. We also found that hatching was delayed in these embryos as compared with matched controls. Moreover, the frequency of contraction and expansion movements after blastocyst formation was significantly higher in the blastomere removal group as compared with the control group. Finally, the maximum diameter of the expanded blastocyst just before hatching was not significantly different between both groups. These findings suggested that blastomere removal has an adverse effect on embryonic development around the time of hatching. Thus, future developments in preimplantation genetic diagnosis and screening should involve further consideration and caution in light of the influence of blastomere biopsy on embryonal growth. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  11. Development of an embryo toxicity test with the pond snail Lymnaea stagnalis using the model substance tributyltin and common solvents.

    PubMed

    Bandow, Cornelia; Weltje, Lennart

    2012-10-01

    The development of a chronic mollusc toxicity test is a current work item on the agenda of the OECD. The freshwater pond snail Lymnaea stagnalis is one of the candidate snail species for such a test. This paper presents a 21-day chronic toxicity test with L. stagnalis, focussing on embryonic development. Eggs were collected from freshly laid egg masses and exposed individually until hatching. The endpoints were hatching success and mean hatching time. Tributyltin (TBT), added as TBT-chloride, was chosen as model substance. The selected exposure concentrations ranged from 0.03 to 10 μg TBT/L (all as nominal values) and induced the full range of responses. The embryos were sensitive to TBT (the NOEC for mean hatching time was 0.03 μg TBT/L and the NOEC for hatching success was 0.1 μg TBT/L). In addition, data on maximum limit concentrations of seven common solvents, recommended in OECD aquatic toxicity testing guidelines, are presented. Among the results, further findings as average embryonic growth and mean hatching time of control groups are provided. In conclusion, the test presented here could easily be standardised and is considered useful as a potential trigger to judge if further studies, e.g. a (partial) life-cycle study with molluscs, should be conducted. Copyright © 2012 Elsevier B.V. All rights reserved.

  12. Marked depression of time interval between fertilization period and hatching period following exposure to low-dose X-rays in zebrafish.

    PubMed

    Miyachi, Yukihisa; Kanao, Tomoko; Okamoto, Takehito

    2003-10-01

    In recent years there has been growing concern over the stimulating effects of very low-dose X-rays. Our laboratory had observed that zebrafish irradiated with low-dose X-rays tended to emerge earlier than sham controls. This observation led us to quantitatively examine the effects of low-dose X irradiation on a series of stages of development in the zebrafish. The embryos were fertilized simultaneously in vitro and incubated at an optimal temperature without crowding. Following exposure of the cleavage period (1.5 h after fertilization) to 0.025-Gy X-rays, the duration to hatching was slightly shorter than that of the sham controls. This tendency was increased when the X-ray exposure occurred during the blastula period (3.5 h). In these embryos, the duration to hatching decreased significantly by an average of 6 h sooner than for sham controls. No differences in duration to hatching were seen when irradiation was given during either the zygote period (45 min) or the segmentation period (12 h). On the contrary, upon exposure to 0.5-Gy X-rays during the blastula period, the duration to hatching increased significantly relative to that of sham controls. These results suggest that the radiation-induced early hatching effect is observed for low doses of X-rays.

  13. Productive performance, egg quality, and hatching traits of Japanese quail reared under different levels of glycerin.

    PubMed

    Ghayas, A; Hussain, J; Mahmud, A; Javed, K; Rehman, A; Ahmad, S; Mehmood, S; Usman, M; Ishaq, H M

    2017-07-01

    This study evaluated subsequent effects of glycerin on productive performance, egg quality, and hatching traits in Japanese quail. A total of 200 birds was arranged according to a completely randomized design into 5 treatment groups having 5 replicates of 8 birds each (6 females and 2 males). Treatments consisted 5 levels of glycerin, i.e., 2.5, 5, 7.5, and 10% and the control group. Birds were fed with different levels of glycerin during a rearing period of 6 wk and their subsequent effects on productive performance, egg quality, and hatching traits were observed. Data were collected regarding productive performance for 16 wk; however, egg quality and hatching traits were recorded during pre-peak (at fourth wk), peak (at 12th wk), and post peak (at 16th wk) phase. Productive performance, egg quality, and hatching traits did not differ significantly throughout the experimental period. It was concluded that glycerin can be used as a replacement energy source, having no effect on productive and reproductive performance in Japanese quail. © 2017 Poultry Science Association Inc.

  14. Optimal Divergence-Free Hatch Filter for GNSS Single-Frequency Measurement.

    PubMed

    Park, Byungwoon; Lim, Cheolsoon; Yun, Youngsun; Kim, Euiho; Kee, Changdon

    2017-02-24

    The Hatch filter is a code-smoothing technique that uses the variation of the carrier phase. It can effectively reduce the noise of a pseudo-range with a very simple filter construction, but it occasionally causes an ionosphere-induced error for low-lying satellites. Herein, we propose an optimal single-frequency (SF) divergence-free Hatch filter that uses a satellite-based augmentation system (SBAS) message to reduce the ionospheric divergence and applies the optimal smoothing constant for its smoothing window width. According to the data-processing results, the overall performance of the proposed filter is comparable to that of the dual frequency (DF) divergence-free Hatch filter. Moreover, it can reduce the horizontal error of 57 cm to 37 cm and improve the vertical accuracy of the conventional Hatch filter by 25%. Considering that SF receivers dominate the global navigation satellite system (GNSS) market and that most of these receivers include the SBAS function, the filter suggested in this paper is of great value in that it can make the differential GPS (DGPS) performance of the low-cost SF receivers comparable to that of DF receivers.

  15. Optimal Divergence-Free Hatch Filter for GNSS Single-Frequency Measurement

    PubMed Central

    Park, Byungwoon; Lim, Cheolsoon; Yun, Youngsun; Kim, Euiho; Kee, Changdon

    2017-01-01

    The Hatch filter is a code-smoothing technique that uses the variation of the carrier phase. It can effectively reduce the noise of a pseudo-range with a very simple filter construction, but it occasionally causes an ionosphere-induced error for low-lying satellites. Herein, we propose an optimal single-frequency (SF) divergence-free Hatch filter that uses a satellite-based augmentation system (SBAS) message to reduce the ionospheric divergence and applies the optimal smoothing constant for its smoothing window width. According to the data-processing results, the overall performance of the proposed filter is comparable to that of the dual frequency (DF) divergence-free Hatch filter. Moreover, it can reduce the horizontal error of 57 cm to 37 cm and improve the vertical accuracy of the conventional Hatch filter by 25%. Considering that SF receivers dominate the global navigation satellite system (GNSS) market and that most of these receivers include the SBAS function, the filter suggested in this paper is of great value in that it can make the differential GPS (DGPS) performance of the low-cost SF receivers comparable to that of DF receivers. PMID:28245584

  16. Snapping turtles (Chelydra serpentina) as bioindicators in Canadian areas of concern in the Great Lakes Basin. II. Changes in hatching success and hatchling deformities in relation to persistent organic pollutants.

    PubMed

    de Solla, S R; Fernie, K J; Ashpole, S

    2008-06-01

    Hatching success and deformities in snapping turtle hatchlings (Chelydra serpentina) were evaluated using eggs collected from 14 sites in the Canadian lower Great Lakes, including Areas of Concern (AOC), between 2001 and 2004. Eggs were analyzed for PCBs, PBDEs, and pesticides. Between 2002 and 2004, hatchling deformity rates were highest in two AOCs (18.3-28.3%) compared to the reference sites (5.3-11.3%). Hatching success was poorest in three AOCs (71.3-73.1%) compared to the reference sites (86.0-92.7%). Hatching success and deformity rates were generally poorer in 2001 compared to 2002-2004, irrespective of the study location and could be due to egg handling stress in 2001. Hatching success and deformities were generally worst from the Wheatley Harbour, St. Lawrence River (Cornwall), Detroit River, and Hamilton Harbour AOCs. Associations between contaminant burdens with embryonic development were sufficiently poor that the biological relevance is questionable. Stressors not measured may have contributed to development abnormalities.

  17. High duck nesting success in a predator-reduced environment

    USGS Publications Warehouse

    Duebbert, H.F.; Lokemoen, J.T.

    1980-01-01

    Duck nesting and production were studied during 1969-74 on a 51-ha field of undisturbed grass-legume cover and a surrounding 8.13-km2 area in north-central South Dakota. The principal mammalian predators of ducks were reduced within a 259-km2 zone from May 1969 through August 1971. Dabbling duck nest densities, hatching success, and breeding populations attained high levels. Seven duck species produced 1,062 nests on the 51-ha field during 6 years, 864 (81%) hatched, 146 (14%) were destroyed, and 52 (5%) had other fates. During 1970-72, when predator reduction was most effective, the hatching success for 756 nests was 94%. The number of mallard (Anas platyrhynchos) nests increased from 37 (0.7/ha) in 1969 to 181 (3.5/ha) in 1972. Mallard pairs increased from 2.8/km2 to 16.8/km2 on the 8.13-km2 area during the same period. A minimum of 7,250 ducklings hatched on the 51-ha field during the 6 years, including 2,342 ducklings in 1972. Exceptionally high duck nesting densities and hatching rates occurred when predators were controlled.

  18. Temperature effects on egg development and larval condition in the lesser sandeel, Ammodytes marinus

    NASA Astrophysics Data System (ADS)

    Régnier, Thomas; Gibb, Fiona M.; Wright, Peter J.

    2018-04-01

    Understanding the influence of temperature on egg development and larval condition in planktonic fish is a prerequisite to understanding the phenological impacts of climate change on marine food-webs. The lesser sandeel, Ammodytes marinus (Raitt 1934), is a key trophic link between zooplankton and many piscivorous fish, sea birds and mammals in the northeast Atlantic. Temperature-egg development relationships were determined for batches of lesser sandeel eggs. Hatching began as early as 19 days post fertilisation at 11 °C and as late as 36 days post fertilisation at 6 °C, which is faster than egg development rates reported for closely related species at the lower end of the tested temperature range. The average size of newly hatched larvae decreased with increasing incubation temperatures in early hatching larvae, but this effect was lost by the middle of the hatching period. While the study revealed important temperature effects on egg development rate, predicted variability based on the range of temperatures eggs experience in the field, suggests it is only a minor contributor to the observed inter-annual variation in hatch date.

  19. Evidence for Vertical Transmission of Novel Duck-Origin Goose Parvovirus-Related Parvovirus.

    PubMed

    Chen, H; Tang, Y; Dou, Y; Zheng, X; Diao, Y

    2016-06-01

    In 2015, novel duck-origin goose parvovirus-related parvovirus (N-GPV) infection progressively appeared in commercial Cherry Valley duck flocks in North China. Diseased ducks were observed to have beak atrophy and dwarfism syndrome (BADS). A previous study showed that a high seropositive rate for N-GPV indicated a latent infection in most breeder duck flocks. To investigate this possibility in hatching eggs collected from N-GPV-infected breeder ducks, 120 eggs were collected at various stages of embryonic development for viral DNA detection and an N-GPV-specific antibody test. N-GPV DNA was present in nine hatching eggs, eleven duck embryo and eight newly hatched ducklings. Of the newly hatched ducklings, 58.33% (21/36) were seropositive. Further, two isolates were obtained from a 12-day-old duck embryo and a newly hatched duckling. N-GPV infection did not reduce the fertilization rate and hatchability. These results indicate possible vertical transmission of N-GPV and suggest that it may be transmitted from breeder ducks to ducklings in ovo. © 2016 Blackwell Verlag GmbH.

  20. [Assisted hatching following embryo implantation failure].

    PubMed

    Carballo Mondragón, Esperanza; Durán Monterrosas, Leonor; Campos Cañas, Jorge A; González de Jesús, Patricia; Kably Ambe, Alberto

    2012-08-01

    Assisted hatching in reproduction techniques has improved the successful implantation rates in certain groups of patients with poor prognosis. This study focuses on its effect in groups of patients with previous implantation failure and according to age groups. Compare the pregnancy rates of patients who turned to this technique following an implantation failure using in vitro fertilization with those of patients who did not use assisted hatching before another attempt of in vitro fertilization and according to specific age groups. Cases of patients using assisted hatching in our Center between January 2008 and December 2009 were studied. The results were compared in terms of age in three groups: group I, >35 years; group II, 35-39 years, and group III, > 40 years. Patients in group II had better pregnancy rate (30%) than those in groups I and III (16.98 and 20.83%, respectively). When comparing the results of the group of patients using assisted hatching with those of the group that did not, the first reported a 20% pregnancy rate versus no pregnancy in the other group.

  1. The Hatch-Smolensk exchange

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sproles, A.

    1993-03-01

    During summer 1992, the World Association of Nuclear Operators (WANO) sponsored an exchange visit between Georgia Power Company's Edwin I. Hatch nuclear plant, a two-unit boiling water reactor site, and the Smolensk atomic energy station, a three-unit RBMK (graphite-moderated and light-water-cooled) plant located 350 km west of Moscow, in Desnogorsk, Russia. The Plant Hatch team included Glenn Goode, manager of engineering support; Curtis Coggin, manager of training and emergency preparedness; Wayne Kirkley, manager of health physics and chemistry; John Lewis, manager of operations; Ray Baker, coordinator of nuclear fuels and contracts; and Bruce McLeod, manager of nuclear maintenance support. Alsomore » traveling with the team was Jerald Towgood, of WANO's Atlanta Centre. The Hatch team visited the Smolensk plant during the week of July 27, 1992.« less

  2. STS-54 Commander Casper at airlock hatch on CCT middeck during JSC training

    NASA Technical Reports Server (NTRS)

    1992-01-01

    STS-54 Endeavour, Orbiter Vehicle (OV) 105, Commander John H. Casper manipulates the airlock hatch and its equalization valves on the middeck of JSC's Crew Compartment Trainer (CCT). Casper is rehearsing the sequence of events necessary for extravehicular activity (EVA) egress for the upcoming STS-54 mission. Visible in the airlock is an extravehicular mobility unit (EMU). Two of the STS-54 crewmembers will don EMUs and egress through the EV hatch into the payload bay (PLB) after Casper closes the intravehicular (IV) hatch behind them. The EVA crewmembers will spend four-plus hours on a planned spacewalk to evaluate EVA techniques and gear for the Space Station Freedom (SSF). The CCT is located in JSC's Mockup and Integration Laboratory (MAIL) Bldg 9NE.

  3. 9 CFR 147.22 - Hatching egg sanitation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AGRICULTURE LIVESTOCK IMPROVEMENT AUXILIARY PROVISIONS ON NATIONAL POULTRY IMPROVEMENT PLAN Sanitation... soiled nest eggs may be gently dry cleaned by hand. (c) Hatching eggs should be stored in a designated...

  4. Structural analysis of hatch cover plates on FMEF high bay mezzanine

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dixson, G.E.

    1997-05-29

    In order to move the Idaho National Engineering Laboratory (INEL) Light Duty Utility Arm (LDUA) trailer into position for testing on the Fuels and Materials Examination Facility (FMEF) 42 ft level mezzanine one of the trailer`s wheels will have to sit on a circular hatch cover fabricated from one-inch thick steel plate. The attached calculations verify that the hatch cover plate is strong enough to support the weight of the INEL LDUA trailer`s wheel.

  5. STS-47 Commander Gibson and Pilot Brown at CCT side hatch during JSC training

    NASA Technical Reports Server (NTRS)

    1992-01-01

    STS-47 Endeavour, Orbiter Vehicle (OV) 105, Spacelab Japan (SLJ) Commander Robert L. Gibson (right) and Pilot Curtis L. Brown, Jr, wearing launch and entry suits (LESs), pose in front of the Crew Compartment Trainer (CCT) mockup side hatch during post landing emergency egress procedures held at JSC's Mockup and Integration Laboratory (MAIL) Bldg 9NE. Note that the crew escape system (CES) pole is in position at side hatch but is not extended.

  6. STS-48 Commander Creighton, in LES, stands at JSC FFT side hatch

    NASA Technical Reports Server (NTRS)

    1991-01-01

    STS-48 Discovery, Orbiter Vehicle (OV) 103, Commander John O. Creighton, wearing a launch and entry suit (LES), stands at the side hatch of JSC's full fuselage trainer (FFT). Creighton will enter the FFT shuttle mockup through the side hatch and take his assigned position on the forward flight deck. Creighton, along with the other crewmembers, is participating in a post-landing emergency egress exercise. The FFT is located in the Mockup and Integration Laboratory (MAIL) Bldg 9A.

  7. Fuglesang at hatch

    NASA Image and Video Library

    2009-08-31

    ISS020-E-037052 (31 Aug. 2009) --- European Space Agency astronaut Christer Fuglesang, STS-128 mission specialist, works near a hatch on the International Space Station while Space Shuttle Discovery remains docked with the station.

  8. Next-Generation MKIII Lightweight HUT/Hatch Assembly

    NASA Technical Reports Server (NTRS)

    McCarthy, Mike; Toscano, Ralph

    2013-01-01

    The MK III (H-1) carbon-graphite/ epoxy Hard Upper Torso (HUT)/Hatch assembly was designed, fabricated, and tested in the early 1990s. The spacesuit represented an 8.3 psi (˜58 kPa) technology demonstrator model of a zero prebreathe suit. The basic torso shell, brief, and hip areas of the suit were composed of a carbon-graphite/epoxy composite lay-up. In its current configuration, the suit weighs approximately 120 lb (˜54 kg). However, since future planetary suits will be designed to operate at 0.26 bar (˜26 kPa), it was felt that the suit's re-designed weight could be reduced to 79 lb (˜35 kg) with the incorporation of lightweight structural materials. Many robust, lightweight structures based on the technologies of advanced honeycomb materials, revolutionary new composite laminates, metal matrix composites, and recent breakthroughs in fullerene fillers and nanotechnology lend themselves well to applications requiring materials that are both light and strong. The major problem involves the reduction in weight of the HUT/ Hatch assembly for use in lunar and/or planetary applications, while at the same time maintaining a robust structural design. The technical objective is to research, design, and develop manufacturing methods that support fa b rica - tion of a lightweight HUT/Hatch assembly using advanced material and geometric redesign as necessary. Additionally, the lightweight HUT/Hatch assembly will interface directly with current MK III hardware. Using the new operating pressure and current MK III (H-1) interfaces as a starting block, it is planned to maximize HUT/Hatch assembly weight reduction through material selection and geometric redesign. A hard upper torso shell structure with rear-entry closure and corresponding hatch will be fabricated. The lightweight HUT/Hatch assembly will retrofit and interface with existing MK III (H-1) hardware elements, providing NASA with immediate "plug-andplay" capability. NASA crewmembers will have a lightweight, robust, life-support system that will minimize fatigue during extraterrestrial surface sojourns. Its unique feature is the utilization of a new and innovative family of materials used by the aerospace industry, which at the time of this reporting has not been used for the proposed application.

  9. Influence of chick hatch time and access to feed on broiler muscle development.

    PubMed

    Powell, D J; Velleman, S G; Cowieson, A J; Singh, M; Muir, W I

    2016-06-01

    The effect of hatch time and the timing of access to feed on growth rate and breast muscle development was assessed in Ross 308 broiler chickens. Chicks were removed from the incubator upon hatching, and classified as early (EH), midterm (MH), or late (LH) hatchers, based on the duration of their incubation. Feed and water were available either immediately at hatch, or 24 h after the conclusion of the hatch period. Hatchling body weight was uniform regardless of hatch time. Subsequently, bodyweight was increased in EH compared to LH birds following immediate access to feed, until 7 d in female, and 14 d in male birds. Relative breast weight was increased until 28 d in birds with immediate access to feed, and also EH and MH birds regardless of access to feed. Pectoralis major muscle morphology and expression of the myogenic regulatory factors myogenic determination factor 1 (MYOD1) and myogenin, and the proteoglycans syndecan-4, glypican-1, and decorin were measured. Myogenin and glypican-1 stimulate satellite cell (SC) differentiation. Glypican-1 expression was unaffected by treatment. A late increase in myogenin expression was observed in MH birds with delayed access to feed, and all LH birds. Syndecan-4 and MYOD1, expressed in proliferating SC, and decorin, which stimulates satellite cell proliferation and differentiation, were variably upregulated in the first wk posthatch in the same birds. These data suggest SC were activated and proliferating, but had reduced differentiation in later hatching and feed deprived birds. Conversely, EH birds with immediate access to feed had maximal myofiber width at 7 d, while fiber width was increased in birds with immediate access to feed compared to those with delayed access to feed through 40 d of age. These results demonstrate that delaying chick access to feed for 24 h upon removal from the incubator will impair muscle growth. Additionally, hatch time influences muscle development, with accelerated muscle growth in EH and MH, compared to LH birds, irrespective of access to feed. © 2016 Poultry Science Association Inc.

  10. Effects of maternal dietary EPA and DHA supplementation and breeder age on embryonic and post-hatch performance of broiler offspring: age and n-3 pufa affect embryonic and post-hatch performance.

    PubMed

    Koppenol, A; Delezie, E; Wang, Y; Franssens, L; Willems, E; Ampe, B; Buyse, J; Everaert, N

    2015-04-01

    Breeder age and nutrition are amongst the most important factors affecting progeny growth and development. The present experiment was carried out to evaluate the effects of n-3 fatty acid (FA), with special emphasis on the ratio of eicosapentaenoic (EPA, 20:5 n-3) and docosahexaenoic (DHA, 22:6 n-3) acid, provided to the diet of ageing broiler breeder hens at different ratios, on the incubation parameters and the performance of the offspring. Four hundred and eighty Ross-308 broiler breeder hens were fed one of four different diets (120/treatment), with an equal fat content. The control diet was a basal diet, rich in n-6 FAs (CON). Blends of fish oil were used to enrich the three other diets in n-3 FA and to obtain different EPA/DHA ratios of 1/1 (EPA=DHA), 1/2 (DHA) or 2/1 (EPA). Every 5 weeks, incubation parameters were assessed. Every 15 weeks, offspring was reared until slaughter age on a standard diet. Breeder age affected almost all incubation and post-hatch parameters, whereas n-3 FA treatment only lowered egg weight (p < 0.0001) and consequently hatched chick weight (p < 0.0001). Supplementation of EPA resulted in a higher proportional liver weight (p = 0.0219) at hatch, a lower body weight up to 28 days post-hatch (p = 0.0418), a lower daily weight gain (p = 0.0498) and a higher feed conversion ratio (p = 0.0395) during the starter period (p = 0.0498), resulting in a higher overall offspring feed conversion ratio (p = 0.0317) compared to the control diet. DHA supplementation, on the other hand, resulted in a lower residual yolk weight (p = 0.0220) and a higher overall offspring mortality (p = 0.0125). In conclusion, supplementation of n-3 FA could not counter the adverse effect of breeder flock age, but did not harm incubation or improve post-hatch performance, either. EPA and DHA affected offspring development differently during early post-hatch life. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  11. Effects of moment of hatch and feed access on chicken development.

    PubMed

    Lamot, D M; van de Linde, I B; Molenaar, R; van der Pol, C W; Wijtten, P J A; Kemp, B; van den Brand, H

    2014-10-01

    The current study evaluated effects of hatch moment and immediate feed and water access within a 24-h hatch window on chicken growth and development. Five hundred four male chickens obtained from a 49-wk-old Ross 308 breeder flock were assigned to 72 cages based on hatching moment (early, midterm, or late; selected during periods of 475 to 481, 483 to 487, and 489 to 493 h after onset of incubation). At the end of each hatching period, chickens were moved to the grow-out facility and one-half of the chickens received feed and water ad libitum immediately. Remaining chickens received feed and water from 504 h after onset of incubation (d 0). Body weight gain and feed intake for each cage were recorded at d 0, 1, 4, 7, 11, and 18. Chickens were sampled at d 4 and 18 for organ and carcass development. Early hatchers had lower BW at placement compared with midterm and late hatchers but compensated for this afterward, resulting in a higher BW at d 4 (112.8, 107.1, and 103.3 g, respectively). From d 0 to 18, early hatchers tended to have higher BW gain than both other groups. Relative breast meat yield at d 18, expressed as percentage of carcass weight, was higher for early (30.4%) than midterm (28.5%) and late hatchers (27.8%). Up to d 7, direct feed access resulted in higher BW gain (6.1%) and feed intake (4.2%) compared with delayed feed access. No effect of moment of feed access on feed efficiency or organ weights was found. Direct feed access resulted in a higher weight:length ratio of the jejunum (12.5%) and ileum (7.5%) at d 4 compared with delayed feed access. These results suggest that early hatchers have a different developmental and growth pattern than midterm or late hatchers within a 24-h hatch window. A mild delay in feed access after hatch affects growth and development during the first week after hatch. ©2014 Poultry Science Association Inc.

  12. Comparison of hatching egg characteristics, embryo development, yolk absorption, hatch window, and hatchability of Pekin Duck eggs of different weights.

    PubMed

    Ipek, A; Sozcu, A

    2017-10-01

    This study was carried out to determine the hatching egg characteristics, embryo development and yolk absorption during incubation, hatch window, and hatchability of Pekin duck eggs of different weights. A total of 960 hatching eggs was obtained from a breeder flock 35 to 36 wk of age. The eggs were classed into 3 weight categories: "light" (L; <75 g), "medium" (M; 76 to 82 g), and "heavy" (H; >83 g). The albumen weight was the highest in the heavy eggs, whereas the yolk weight was higher in the medium and heavy eggs. Egg breaking strength was the highest with a value of 2.5 kg/cm2 in light eggs, whereas the thinnest eggshell (0.3862 mm) was observed in heavy eggs. pH of albumen and yolk was similar and ranged from 8.8 to 8.9 and 5.9 to 6.0, respectively. On d 14 of incubation, yolk sac weight was found higher in the medium and heavy eggs. Additionally, the dry matter of the embryo and yolk sac differed among the egg weight groups during the incubation period. Interestingly, on d 25 of incubation, the embryo weight was higher in the light and heavy eggs (35.2 and 36.3 g, respectively) than in the medium eggs (29.8 g). These findings showed that embryo growth was affected by yolk absorption and dry matter accumulation. The hatchability of total and fertile eggs was lower for the heavy eggs than the light and medium eggs. The chick weight was 42.8, 48.4, and 54.9 g in light, medium, and heavy eggs, respectively. A percentage of 34.2, 36, and 31.6% of chicks from light, medium, and heavy eggs, hatched between 637 and 648 h, 39.6, 36.2, and 32.9% between 649 and 660 h, 26.2, 27.8, and 35.5% between 661 and 672 h of incubation, respectively. In conclusion, hatching egg quality, embryo development and yolk absorption during incubation, hatch window, and hatchability were affected by egg weight in Pekin ducks. © 2017 Poultry Science Association Inc.

  13. Evaluation of disinfectants and antiseptics to eliminate bacteria from the surface of turkey eggs and hatch gnotobiotic poults.

    PubMed

    Sylte, M J; Chandra, L C; Looft, T

    2017-07-01

    Bird eggs are in contact with intestinal microbiota at or after oviposition, but are protected from bacterial translocation by a glycoprotein cuticle layer, the shell, and internal membranes. In a preliminary study, turkey eggs were hatched in a germ-free environment. Firmicutes 16S rRNA gene was detected in the cecal microbiota of hatched poults, suggesting that poults may acquire spore-formers by exposure to shell contents during hatching. Generating gnotobiotic poults for research requires elimination of bacteria from the egg's surface without damaging the developing embryo. The ability of different disinfectants and antiseptics to eliminate eggshell bacteria without harming the developing embryo was tested. Different classes of disinfectants and antiseptics (halogens, biguanidines, and oxidants) were selected to target spores and vegetative bacteria likely present on the egg's surface. Eggs were treated by fully immersing in heated antiseptic (betadine or chlorhexidine) or disinfectant (alkaline bleach, acidified bleach, chlorine dioxide, Oxysept-333, or Virkon S) solutions for up to 15 minutes. Shells were aseptically harvested for aerobic and anaerobic culturing of bacteria. Toxicity to the developing embryo was assessed by gross evaluation of developmental changes in treated eggs incubated up to 27 d of embryonation. Halogen disinfectants acidified bleach and chlorine dioxide, and oxidants Oxysept-333 and Virkon-S eliminated viable bacteria from eggshells. However, addition of oxidants, alone or in combination with other treatments, produced significant (P < 0.05) embryotoxicity. The combination treatment of acidified bleach, chlorine dioxide, and betadine produced minimal embryotoxicity and eliminated viable bacteria from whole turkey eggs, and produced hatched poults in a gnotobiotic isolator. As a control, eggs were treated with PBS, incubated, and hatched under germ-replete conditions. After hatching, poults were euthanized and treated poults had no detectable bacterial growth or 16S rRNA gene qPCR amplification, demonstrating that acidified sodium hypochlorite, chlorine dioxide, and betadine safely hatched gnotobiotic poults. Generation of germ-free poults is an important tool and will be used to evaluate the host-pathogen interaction by foodborne pathogens such as Campylobacter spp. Published by Oxford University Press on behalf of Poultry Science Association 2017.

  14. Effect of hen age and maternal vitamin D source on performance, hatchability, bone mineral density, and progeny in vitro early innate immune function.

    PubMed

    Saunders-Blades, J L; Korver, D R

    2015-06-01

    The metabolite 25-hydroxy vitamin D3 (25-OHD) can complement or replace vitamin D3 in poultry rations, and may influence broiler production and immune function traits. The effect of broiler breeder dietary 25-OHD on egg production, hatchability, and chick early innate immune function was studied. We hypothesized that maternal dietary 25-OHD would support normal broiler breeder production and a more mature innate immune system of young chicks. Twenty-three-week-old Ross 308 hens (n=98) were placed in 4 floor pens and fed either 2,760 IU vitamin D3 (D) or 69 μg 25-OHD/kg feed. Hen weights were managed according to the primary breeder management guide. At 29 to 31 wk (Early), 46 to 48 wk (Mid), and 61 to 63 wk (Late), hens were artificially inseminated and fertile eggs incubated and hatched. Chicks were placed in cages based on maternal treatment and grown to 7 d age. Innate immune function and plasma 25-OHD were assessed at 1 and 4 d post-hatch on 15 chicks/treatment. Egg production, hen BW, and chick hatch weight were not affected by diet (P>0.05). Total in vitro Escherichia coli (E. coli) killing by 25-OHD chicks was greater than the D chicks at 4 d for the Early and Mid hatches, and 1 and 4 d for the Late hatch. This can be partly explained by the 25-OHD chicks from the Late hatch also having a greater E. coli phagocytic capability. No consistent pattern of oxidative burst response was observed. Chicks from the Mid hatch had greater percent phagocytosis, phagocytic capability, and E. coli killing than chicks from Early and Late hatches. Overall, maternal 25-OHD increased hatchability and in vitro chick innate immunity towards E. coli. Regardless of treatment, chicks from Late and Early hens had weaker early innate immune responses than chicks from Mid hens. The hen age effect tended to be the greatest factor influencing early chick innate immunity, but maternal 25-OHD also increased several measures relative to D. © 2015 Poultry Science Association Inc.

  15. TIME-TEMPERATURE RELATIONS IN THE INCUBATION OF THE WHITEFISH, COREGONUS CLUPEAFORMIS (MITCHILL)

    PubMed Central

    Price, John W.

    1940-01-01

    1. Whitefish eggs incubated in aerated lake water at controlled tempera tures of 0°, 0.5°, 2°, 4°, 6°, 8°, 10°, and 12°C., failed to hatch at either 0° or 12°C. 0.6 per cent hatched alive at 10°C., 72.67 per cent hatched alive at 0.5°C., and an intermediate proportion hatched at intermediate temperatures. 2. The percentage of abnormal embryos which developed to the hatching stage varied directly with temperature between 4° and 12°, all embryos being abnormal at 12°C.; but none were abnormal at either 0.5°, or 2°C. Normal development predominated from 0.5 to 6°C. The highest proportion of embryos to hatch alive was 72.67 per cent at 0.5°C., which is, hence, the optimum temperature. 3. Total incubation time ranged from 29.6 days at 10°C. to 141 days at 0.5°C. 4. The time (T) required to attain any given stage of development is expressed in equations See PDF for Equation where temperature, t, is a negative exponent of the constant, A, whose value differs above or below 6°C., a critical temperature. Values of A above 6° fluctuate about 1.13; those of A below 6° fluctuate about 1.19 as a mean. 5. Applying Arrhenius' equation µ values for the total incubation period are 27,500 below 6° and 27,100 above it. 6. The relative magnitude of A values of the exponential equation and µ values of Arrhenius' equation show corresponding changes from one developmental period to another. 7. When plotted, thermal increments show cyclic variations, with maxima during periods of cleavage and of organogenesis. These may indicate the interaction of two separate sets of embryonic processes, which give a maximal response to temperature differences during these two separate periods. 8. Above 6°, µ values during the hatching process are distinct from those of developmental stages and are regarded as being due to the action of hatching enzymes. PMID:19873168

  16. Zinc oxide nanoparticle toxicity in embryonic zebrafish: Mitigation with different natural organic matter.

    PubMed

    Kteeba, Shimaa M; El-Adawi, Hala I; El-Rayis, Osman A; El-Ghobashy, Ahmed E; Schuld, Jessica L; Svoboda, Kurt R; Guo, Laodong

    2017-11-01

    Exposure experiments were conducted to evaluate the influence of dissolved organic matter (DOM) on the toxicity of ZnO-NPs (10-30 nm) and dissolved Zn at sub-lethal doses (50 and 5 ppm, respectively) to zebrafish (Danio rerio). Humic acid, alginic acid, bovine serum albumin and various natural DOM isolated from rivers as the Milwaukee River-WI (NOMW), Yukon River-AK (NOMA) and Suwannee River-GA DOM (NOMS) were used to represent humic substances (HA), carbohydrates (CHO), proteins (PTN), and natural organic matter (NOM), respectively. Initial experiments were carried out to confirm the toxic effect of ZnO-NPs at 50 ppm, followed by mitigation experiments with different types and concentrations of DOM (0.4-40 mg-C/L). Compared to 0% hatch of 50 ppm ZnO-NPs exposed embryos at 72 h post fertilization (hpf), NOMS, NOMW and HA had the best mitigative effects on hatching (53-65%), followed by NOMA, CHO and PTN (19-35%); demonstrating that the mitigation effects on ZnO-NPs toxicity were related to DOM's quantity and composition. At 96 hpf, 20% of embryos exposed to 50 ppm ZnO-NPs hatched, 100% of embryos reared in embryo medium hatched, and close to 100% of the embryos hatched upon mitigation, except for those mitigated with PTN which had less effect. Dissolved Zn (5 ppm) also exhibited the same toxicity on embryos as ZnO-NPs (50 ppm). However, in the presence of HA, NOM and CHO, the hatching rates at 72 and 96 hpf increased significantly compared to 5% hatch without DOM. The overall mitigation effects produced by DOM followed the order of HA ≥ NOMS > NOM (A&W) > CHO > PTN, although specific mitigation effects varied with DOM concentration and functionalities. Our results also indicate that the toxicity of ZnO-NPs to embryos was mostly derived from NPs although dissolved Zn released from ZnO-NPs also interacted with embryos, affecting hatching, but to a less extent. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. 29 CFR 1918.91 - Housekeeping.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... safe reach of employees. (e) Coaming clearance. Dunnage, hatch beams, tarpaulins or gear not in use... hatch coaming. (f) Nails. (1) Nails that are protruding from shoring or fencing in the work area shall...

  18. 29 CFR 1918.91 - Housekeeping.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... safe reach of employees. (e) Coaming clearance. Dunnage, hatch beams, tarpaulins or gear not in use... hatch coaming. (f) Nails. (1) Nails that are protruding from shoring or fencing in the work area shall...

  19. BLDG 10, INTERIOR DETAIL OF WINDOW AND CEILING FEED HATCHES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BLDG 10, INTERIOR DETAIL OF WINDOW AND CEILING FEED HATCHES - Naval Magazine Lualualei, Headquarters Branch, Storage Building, Kolekole Road near Sixty-first Street intersection, Pearl City, Honolulu County, HI

  20. 29 CFR 1918.91 - Housekeeping.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... safe reach of employees. (e) Coaming clearance. Dunnage, hatch beams, tarpaulins or gear not in use... hatch coaming. (f) Nails. (1) Nails that are protruding from shoring or fencing in the work area shall...

  1. 29 CFR 1918.91 - Housekeeping.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... safe reach of employees. (e) Coaming clearance. Dunnage, hatch beams, tarpaulins or gear not in use... hatch coaming. (f) Nails. (1) Nails that are protruding from shoring or fencing in the work area shall...

  2. 46 CFR 169.823 - Openings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... bulkheads, hatches, and openings in the hull must be kept closed during the navigation of the vessel. (b) The master may permit hatches or other openings to be uncovered or opened for reasonable purposes such...

  3. Patrick in Interdeck Access Hatch

    NASA Image and Video Library

    2010-02-09

    S130-E-006314 (9 Feb. 2010) --- NASA astronaut Nicholas Patrick, STS-130 mission specialist, is pictured in the hatch which connects the flight deck and middeck of space shuttle Endeavour during flight day two activities.

  4. 46 CFR 169.823 - Openings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... bulkheads, hatches, and openings in the hull must be kept closed during the navigation of the vessel. (b) The master may permit hatches or other openings to be uncovered or opened for reasonable purposes such...

  5. 76 FR 37106 - Combined Notice of Filings #2

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ...-000. Applicants: Hatch Solar Energy Center I, LLC. Description: Notice of Self-Certification of Exempt Wholesale Generator Status of Hatch Solar Energy Center I, LLC. Filed Date: 06/17/2011. Accession Number...

  6. 46 CFR 169.823 - Openings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... bulkheads, hatches, and openings in the hull must be kept closed during the navigation of the vessel. (b) The master may permit hatches or other openings to be uncovered or opened for reasonable purposes such...

  7. Behnken in Interdeck Access Hatch

    NASA Image and Video Library

    2010-02-08

    S130-E-005229 (8 Feb. 2010) --- NASA astronaut Robert Behnken, STS-130 mission specialist, is pictured in the hatch which connects the flight deck and middeck of space shuttle Endeavour during flight day one activities.

  8. 21 CFR 529.1044b - Gentamicin sulfate solution.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... elimination of the following microorganisms from turkey-hatching eggs: Arizona hinshawii (paracolon... treatment of turkey-hatching eggs only. Eggs which have been dipped in the drug shall not be used for food...

  9. 21 CFR 529.1044b - Gentamicin sulfate solution.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... elimination of the following microorganisms from turkey-hatching eggs: Arizona hinshawii (paracolon... treatment of turkey-hatching eggs only. Eggs which have been dipped in the drug shall not be used for food...

  10. 21 CFR 529.1044b - Gentamicin sulfate solution.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... elimination of the following microorganisms from turkey-hatching eggs: Arizona hinshawii (paracolon... treatment of turkey-hatching eggs only. Eggs which have been dipped in the drug shall not be used for food...

  11. 21 CFR 529.1044b - Gentamicin sulfate solution.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... elimination of the following microorganisms from turkey-hatching eggs: Arizona hinshawii (paracolon... treatment of turkey-hatching eggs only. Eggs which have been dipped in the drug shall not be used for food...

  12. Hatch Integration Testing of a NASA TransHab Derivative Woven Inflatable Module

    NASA Technical Reports Server (NTRS)

    Edgecombe, John; Valle, Gerald

    2009-01-01

    Current options for Lunar habitat architecture include inflatable habitats and airlocks. Inflatable structures can have mass and volume advantages over conventional structures. However, inflatable structures are also perceived to carry additional risk because they are at a lower Technical Readiness Level (TRL) than more conventional metallic structures. The use of inflatable structures for habitation will require large penetrations in the inflatable structure to accommodate hatches and/or windows The Hatch Integration Test is designed to study the structural integrity of an expandable structure with an integrated hatch, and to verify mathematical models of the structure. The TransHab project developed an experimental inflatable module at Johnson Space Center in the 1990's. The TransHab design was originally envisioned for use in Mars Transits but was also studied as a potential habitat for the International Space Station (ISS).

  13. A cicada that ensures its fitness during climate warming by synchronizing its hatching time with the rainy season.

    PubMed

    Moriyama, Minoru; Numata, Hideharu

    2011-12-01

    A shift in phenology due to climate change is associated with some recent changes in populations, as it can disrupt the synchrony between organisms' requirements and resource availability. This conceptual framework has been developed mostly in systems of trophic interactions. Many coincidental changes, however, are involved in trophic interactions, preventing us from describing the direct impact of phenological shifts on fitness consequences. Here we address the phenological relationship in a simple non-trophic interaction to document a causal process of a warming-driven fitness change in a cicada, Cryptotympana facialis, whose numbers increased dramatically in Osaka, Japan in the late 20th century. We show that synchrony of the rainy season and hatching time may have a substantial influence on hatching success, by 1) shifting the time of completion of embryonic development, and 2) supplying water at various intervals. We estimate the change in hatching time over the last eleven decades (1901-2009) based on meteorological records and the temperature-dependent rate of C. facialis embryogenesis. Our estimate shows that hatching had initially occurred after the rainy season, and that warming had advanced it into the rainy season in the late 20th century. The probability of hatching success was markedly variable, and often very low before this synchronization occurred, but became stably high thereafter. Our findings suggest that the stabilizing effect of this synchrony on fitness was indispensable to the recent population increase of C. facialis.

  14. Age-related energy values of meat and bone meal for broiler chickens.

    PubMed

    Adeola, O; Anwar, M N; Abdollahi, M R; Ravindran, V

    2018-07-01

    Six hundred and eighty-four male Ross 308 broiler chicks were used to test the null hypothesis that post hatching age between day 0 and 21 does not affect utilization of energy in meat and bone meal (MBM). There were 6 replicate cages in each of 12 treatments consisting of 3 test diets (0, 40, or 80 g MBM/kg diets) at 4 feeding ages post hatching (day 0 to 7, 6 to 11, 10 to 16, or 15 to 21 post hatching) for a total of 72 cages in a randomized complete block design. Birds were assigned to the 12 treatments on day 0, fed a standard starter diet until they were switched to the test diets on day 0, 6, 10, or 15, and the number of birds per cage for day 0 to 7, 6 to 11, 10 to 16, or 15 to 21 were 12, 10, 8, or 8, respectively. Excreta were collected during the last 3 days of each feeding age post hatching and ileal digesta on the last day of each age. There was no interaction between dietary MBM level and feeding age post hatching for any of the response criteria. Weight gain and gain-to-feed ratio improved linearly (P < 0.001) with increasing dietary MBM and, expectedly, there was an increase (P < 0.001) in weight gain and a reduction (P < 0.001) in gain-to-feed ratio with bird feeding age post hatching. Ileal digestible energy (IDE), apparent metabolizable energy (AME), and nitrogen-corrected AME (AMEn) of the diet linearly increased (P < 0.001) with increasing dietary MBM. There were linear increases in dietary IDE (P < 0.05), AME (P < 0.001), and AMEn (P < 0.001) with bird feeding age post hatching. The IDE, ME, and MEn of MBM were determined by the regression procedure. During day 0 to 7, 6 to 11, 10 to 16, or 15 to 21, energy values increased (P < 0.05) with age and the respective IDE of MBM were 2.852, 2.962, 2.927, or 2.959 kcal/g DM. Corresponding ME of MBM were 2.909, 3.125, 3.083, or 3.075 kcal/g DM and those of MEn were 2.687, 2.887, 2.839, or 2.845 kcal/g DM. These results show that energy values of MBM increase with age post hatching and suggest the same energy value of MBM should not be used in formulating diets of broiler chickens during the first 3 weeks post hatching.

  15. [Verification of the eclossion mark on the sagittal otolith of the larvae of Sardinella aurita (Pisces: Clupeidae)].

    PubMed

    Balza, M A; Marín, B

    2000-12-01

    The study of otolith in larvae is important to determine fish age and growth, essential parameters in the study and management of fisheries resources. In this study, the formation of the hatching mark in Sardinella aurita was verified on ichthyoplankton samples collected off southern Cubagua island, Venezuela, from May 1998 to January 1999. The embryos were kept alive using a culture system until they hatched and daily a group of 10 to 30 larvae were fixed in 95% ethanol. An image analysis system was used to measure morphometric characteristics of larvae and sagittal otoliths. Following are mean values in newly hatched larvae: otolith hatching mark distance from nucleus 4.78 m (I.C. 0.36 m, p 0.05 n = 30), increase width 1.46 m (I.C. 0.17 microm, p 0.05, n = 30) and diameter 14.28 m (IC 1.11 m, p 0.05, n = 30). The mean standard length of larvae at age 0 was 3.31 mm (I.C. 0.08 mm, p 0.05, n = 200). The identification of the hatching mark allows the exact calculation of the number of rings in larvae from the natural environment.

  16. Effects of photoinduced toxicity of fluoranthene on amphibian embryos and larvae

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hatch, A.C.; Burton, G.A. Jr.

    1998-09-01

    Embryos and newly hatched larvae of three amphibian species, the spotted salamander (Ambystoma maculatum), the northern leopard frog (Rana pipiens), and the African clawed frog (Xenopus laevis), were exposed to fluoranthene and ultraviolet (UV) light in two scenarios. Embryos were exposed in a laboratory setting from an early developmental stage through hatching under artificial UV light, and newly hatched larvae were exposed outdoors in varying sunlight intensity levels. Outdoor exposures indicated greater sensitivity in the toxic response than did laboratory exposures. In the laboratory, mortality and malformation of X. laevis were the most sensitive indicators of exposure. Xenopus laevis wasmore » also the most sensitive species tested to the effects of UV light alone. Hatching success of R. pipiens was monitored outdoors and was not a useful predictive endpoint in the determination of photoinduced toxicity; however, newly hatched larvae were sensitive to the effects of photoinduced toxicity. Amybstoma maculatum and X. laevis larvae were affected by low ({micro}g/L) concentrations of fluoranthene in sunlight. These findings suggest that low levels of polycyclic aromatic hydrocarbons could be acting synergistically with environmental factors such as UV light to place young amphibians at risk.« less

  17. In Vitro Anthelmintic Activity of Crude Extracts of Aerial Parts of Cissus quadrangularis L. and Leaves of Schinus molle L. against Haemonchus contortus.

    PubMed

    Zenebe, Selamawit; Feyera, Teka; Assefa, Solomon

    2017-01-01

    Haemonchus contortus, the causative agent of Haemonchosis, is the most economically important parasite in small ruminant production. Control with chemotherapy has not been successful due to rapid emergence of drug-resistant strains. There is a continuous search for alternative leads particularly from plants. The study aimed to evaluate the anthelmintic activity of crude methanolic extracts of leaves of Schinus molle and aerial parts of Cissus quadrangularis against H. contortus. Methods . Adult motility test and egg hatching inhibition assay were employed to investigate the in vitro adulticidal and egg hatching inhibitory effects of the extracts. Higher concentrations of the extracts (10 and 5 mg/ml) had a significantly superior adulticidal activity ( p < 0.05) compared to the negative control and lower concentration levels, which was comparable to albendazole. Similarly, the relative egg hatch inhibition efficacy of S. molle and C. quadrangularis extracts indicated a maximum of 96% and 88% egg hatch inhibition, respectively, within the 48 hrs of exposure at 1 mg/ml. The current study evidenced that the crude methanolic extracts of the plants have promising adulticidal and egg hatching inhibitory effects against H. contortus .

  18. Role of shell diffusion area in incubating eggs at simulated high altitude.

    PubMed

    Weiss, H S

    1978-10-01

    Embryonic development is inhibited when eggs are incubated at 9,100 m (0.3 atm) despite a normoxic environment. The problem apparently relates to respiratory gas exchange occurring by diffusion through gas-filled pores in the shell. Gaseous flux is therefore inversely proportional to ambient pressure and is affected by the physical characteristics of the ambient gas (Chapman-Enskog equation). Excess loss of H2O and CO2 occurs in eggs incubating at altitude and could be detrimental. Such increased loss should be correctable by decreasing diffusion area. This was tested by progressively increasing coverage of the shell with paraffin and incubating at simulated 0.3 ATA (225 Torr) in 100% O2. Uncoated eggs failed to hatch, but numbers of chicks increased with increased coverage. Maximum hatch was an extrapolated 90% of controls at 69% shell coverage. With further coverage, hatch size decreased. Egg weight loss, and estimate of H2O diffusion, was around three times controls in uncoated eggs but decreased linearly with paraffin coverage, reaching near normal at maximum hatch. Reduction of diffusion area to 0.3 normal at maximum hatch generally balanced the increased flux predicted for 0.3 ATA.

  19. Wiseman in hatch between U.S. Lab and Node 1

    NASA Image and Video Library

    2014-05-30

    ISS040-E-006564 (30 May 2014) --- NASA astronaut Reid Wiseman, Expedition 40 flight engineer, floats through the hatch between the Destiny laboratory and the Unity node of the International Space Station.

  20. 7 CFR 23.2 - Administration.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... under the Smith-Lever Act of 1914 and the Hatch Act (as amended), August 11, 1955, the administration of... Hatch Act as required by section 504(b) of the Act. (b) Programs authorized under title V shall be...

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