Refinements of environmental assessment during an outbreak investigation of invasive aspergillosis in a leukemia and bone marrow transplant unit.

PubMed

Thio, C L; Smith, D; Merz, W G; Streifel, A J; Bova, G; Gay, L; Miller, C B; Perl, T M

2000-01-01

To investigate an outbreak of aspergillosis in a leukemia and bone marrow transplant (BMT) unit and to improve environmental assessment strategies to detect Aspergillus. Epidemiological investigation and detailed environmental assessment. A tertiary-care university hospital with a 37-bed leukemia and BMT unit Leukemic or BMT patients with invasive aspergillosis identified through prospective surveillance and confirmed by chart review. We verified the diagnosis of invasive fungal infection by reviewing medical charts of at-risk patients, performing a case-control study to determine risk factors for infection, instituting wet mopping to clean all floors, providing N95 masks to protect patients outside high-efficiency particulate air (HEPA)-filtered areas, altering traffic patterns into the unit, and performing molecular typing of selected Aspergillus flavus isolates. To assess the environment, we verified pressure relationships between the rooms and hallway and between buildings, and we compared the ability of large-volume (1,200 L) and small-volume (160 L) air samplers to detect Aspergillus spores. Of 29 potential invasive aspergillosis cases, 21 were confirmed by medical chart review. Risk factors for developing invasive aspergillosis included the length of time since malignancy was diagnosed (odds ratio [OR], 1.0; P=.05) and hospitalization in a patient room located near a stairwell door (OR, 3.7; P=.05). Two of five A. flavus patient isolates were identical to one of the environmental isolates. The pressure in most of the rooms was higher than in the corridors, but the pressure in the oncology unit was negative with respect to the physically adjacent hospital; consequently, the unit acted essentially as a vacuum that siphoned non-HEPA-filtered air from the main hospital. Of the 78 samples obtained with a small-volume air sampler, none grew an Aspergillus species, whereas 10 of 40 cultures obtained with a large-volume air sampler did. During active construction, Aspergillus spores may have entered the oncology unit from the physically adjacent hospital because the air pressure differed. Guidelines that establish the minimum acceptable pressures and specify which pressure relationships to test in healthcare settings are needed. Our data show that large-volume air samples are superior to small-volume samples to assess for Aspergillus in the healthcare environment.

Efficacy of photocatalytic HEPA filter on microorganism removal.

PubMed

Chuaybamroong, P; Chotigawin, R; Supothina, S; Sribenjalux, P; Larpkiattaworn, S; Wu, C-Y

2010-06-01

This study assessed the application of photocatalytic oxidation (PCO) to the high efficiency particulate air (HEPA) filter for disinfection of airborne microorganisms. Experiments were conducted at two TiO2 loadings (1870 +/- 169 and 3140 +/- 67 mg/m(2)) on the HEPA filter irradiated with UV-A at the intensity of 0.85 +/- 0.18 or 4.85 +/- 0.09 mW/cm(2) under two relative humidity conditions (45 +/- 5% and 75 +/- 5%). Inactivation and penetration of four microorganisms were tested, including Aspergillus niger, Penicillium citrinum, Staphylococcus epidermidis, and Bacillus subtilis. It was found that microorganisms retained on a photocatalytic filter were inactivated around 60-80% and even 100% for S. epidermidis when the PCO reactions occurred. Lower penetration was also found from the photocatalytic filter for all airborne microorganisms. High humidity decreased photocatalysis efficacy. Increasing TiO2 loading or irradiance intensity did not substantially affect its disinfection capability. The high efficiency particulate air filter is used widely to remove particulates and microorganisms from the air stream. However, the filter may become a source of microbes if those retained microorganisms proliferate and re-entrain back into the filtered air. This study demonstrates that such a problem can be handled effectively by using photocatalytic reactions to inactivate those confined microorganisms. A 60-100% microbe reduction can be achieved for a wide variety of microorganisms to provide better indoor air quality for hospitals, offices, and domestic applications.

Protective environment for hematopoietic cell transplant (HSCT) recipients: The Infectious Diseases Working Party EBMT analysis of global recommendations on health-care facilities.

PubMed

Styczynski, Jan; Tridello, Gloria; Donnelly, J Peter; Iacobelli, Simona; Hoek, Jennifer; Mikulska, Malgorzata; Aljurf, Mahmoud; Gil, Lidia; Cesaro, Simone

2018-03-13

International guidelines on protective environment for HSCT recipients proposed a set of 10 global recommendations in 2009 on protective environment (GRPE) concerning hospital room design and ventilation. The EBMT Infectious Diseases Working Party undertook a survey on the status on protective environment for HSCT recipients with the aim of surveying current practices and their agreement with GRPE recommendations. The questionnaire consisted of 37 questions divided into 5 sections about filtration, air changes, maintenance, and the protective environment in rooms and the surrounding unit. Overall, 177 centres (response rate 33%) from 36 countries responded, indicating that 99.4% of patient rooms were equipped with HEPA filters, but only 48.6% of the centre's staff were aware of, and could confirm, regular replacement of filters based on manufacturers' recommendations. Well-sealed rooms were used in terms of windows (70.6%), ceilings (35%), and plumbing pipes (51.4%). The sensor monitors in the patient room used to determine when the HEPA filters require changing were installed only in 18.1% of centres. Only 1 centre fulfilled all 10 GRPE recommendations, while 62 centres fulfilled the 3 level "A" recommendations. In conclusion, HEPA-filtered rooms are available in almost all centres, while fewer centres fulfilled other requirements. Knowledge on the details and maintenance of protective environments in the HSCT setting was inadequate, reflecting a lack of communication between the health personnel involved, hospital infection control and the hospital maintenance services.

Promotion of health-enhancing physical activity in rheumatoid arthritis: a comparative study on healthcare providers in Italy, The Netherlands and Sweden.

PubMed

Brodin, Nina; Hurkmans, Emalie; DiMatteo, Luigi; Nava, Tiziana; Vliet Vlieland, Thea; Opava, Christina H

2015-10-01

The objectives of this study were to compare attitudes, practice of advice, perceived competencies and educational needs related to health-enhancing physical activity (HEPA) in rheumatoid arthritis (RA) among Dutch, Italian and Swedish healthcare providers (HCP) and to explore associations between these factors and age, gender and HEPA levels of HCP. Questionnaires were sent to 2939 HCP, members of their national rheumatology organizations. HEPA was assessed with the Short Questionnaire to Assess Health-Enhancing Physical Activity or the International Physical Activity Questionnaire; attitudes, practice of advice, perceived competencies and educational needs with a 23-item questionnaire. Overall response rate was 33 %. Ninety-five percent of HCP agreed that HEPA is an important health goal in RA. More Swedish HCP had positive attitudes to the attainability and safety of HEPA in RA. There were no differences between countries in practice of advice on HEPA to patients with RA in general or to those with recent onset disease, but more Italian HCP were reluctant to advise HEPA to patients with established disease. Of the total HCP, 36 to 60 % used public health guidelines to advise on HEPA, with Dutch HCP taking less advantage. Still they estimated a higher proportion of patients with RA to follow such advice. Italian HCP perceived their competencies the highest, but were also more interested in education to promote HEPA. Gender, age and HEPA performance had no association with attitudes toward HEPA, while a number of associations were found between these factors and practice of advice and perceived competencies. The differences found between HCP in the three countries might indicate the need for educational initiatives to improve HEPA promotion.

Physician Knowledge and Attitudes About Hepatitis A and Current Practices Regarding Hepatitis A Vaccination Delivery.

PubMed

Nelson, Noele P; Allison, Mandy A; Lindley, Megan C; Brtnikova, Michaela; Crane, Lori A; Beaty, Brenda L; Hurley, Laura P; Kempe, Allison

2017-07-01

To assess physicians': 1) knowledge and attitudes about hepatitis A disease and hepatitis A (HepA) vaccine, 2) child care and school HepA vaccine mandates, 3) practices related to HepA vaccine delivery, 4) factors associated with strongly recommending HepA vaccine to all 1- to 2-year-olds, and 5) feasibility of implementing HepA catch-up vaccination at health maintenance visits. A national survey was conducted among representative networks of pediatricians and family medicine physicians (FMs) from March to June, 2014 via e-mail or mail on the basis of provider preference. Response rates were 81% (356 of 440) among pediatricians and 75% (348 of 464) among FMs. Less than 50% correctly identified that hepatitis A virus (HAV) infection is usually asymptomatic in young children and that morbidity from HAV disease increases with age. Ninety-two percent of pediatricians and 59% of FMs strongly recommend HepA vaccine for all 1- to 2-year-olds. In addition to practice specialty, belief that HepA vaccine is required for kindergarten enrollment was the most robust predictor of strong physician recommendation. Gaps in knowledge regarding HAV infection and hepatitis A recommendations and lack of a strong recommendation for routine HepA vaccination of young children among FMs likely contribute to suboptimal coverage. Closing knowledge gaps and addressing barriers that prevent all physicians from strongly recommending HepA vaccine to 1- to 2-year-olds could help increase HepA vaccine coverage and ultimately improve population protection against HAV infection. Published by Elsevier Inc.

Current and maintained health-enhancing physical activity in rheumatoid arthritis: a cross-sectional study.

PubMed

Demmelmaier, Ingrid; Bergman, Patrick; Nordgren, Birgitta; Jensen, Irene; Opava, Christina H

2013-07-01

To describe and identify the explanatory factors of variation in current and maintained health-enhancing physical activity (HEPA) in persons with rheumatoid arthritis (RA). In this cross-sectional study, current HEPA was assessed with the International Physical Activity Questionnaire and maintained HEPA with the Exercise Stage Assessment Instrument, the latter explicitly focusing on both aerobic physical activity and muscle strength training. Sociodemographic, disease-related, and psychosocial data were retrieved from the Swedish Rheumatology Quality (SRQ) registers and a postal questionnaire. The explained variations in the respective HEPA behaviors were analyzed with logistic regression. In all, 3,152 (58.5%) of 5,391 persons identified as eligible from the SRQ registers responded to the questionnaire. Current HEPA was reported by 69%, and maintained HEPA by 11% of the respondents. The most salient and consistent factors explaining variation in both current and maintained HEPA were self-efficacy, social support, and outcome expectations related to physical activity. To our knowledge, this is the first study exploring maintained physical activity in a large well-defined sample of persons with RA. Our results indicate that a minority perform maintained HEPA, including both aerobic physical activity and muscle strength training, and that psychosocial factors are the most salient and consistent in the explanation of HEPA variation. Copyright © 2013 by the American College of Rheumatology.

Photodynamic efficiency of a chlorophyll-a derivative in vitro and in vivo.

PubMed

Zhang, Xiang-Hua; Zhang, Li-Jun; Sun, Jing-Jian; Yan, Yi-Jia; Zhang, Li-Xin; Chen, Na; Chen, Zhi-Long

2016-07-01

This paper reports the antitumor activity of a chlorophyll-a derivative, 2-[1-hydroxyethyl]-2-devinylpyropheophorbide-a (HEPa). Photophysical characteristics of HEPa were measured. And its cytotoxicity, intracellular localization, biodistribution, efficiency of photodynamic therapy (PDT), histological analysis were investigated using human bile duct carcinoma cells (QBC-939) and QBC-939 tumor bearing BABL/c nude mice as animal model. The results showed that HEPa was localized mainly within the cytoplasmic region and partially in lysosome. Biodistribution of HEPa in QBC-939 tumor bearing BABL/c nude mice showed its fast rate of clearance and high tumor selectivity. In vitro, HEPa had low dark toxicity and high photoxicity against QBC-939 cells. The inhibition rate of QBC-939 tumor could increase up to 92.3%, and H&E staining confirmed that HEPa could cause serious damage to the tumor with light dose of 100J/cm(2), implying that HEPa has potential to be a new antitumor candidate for photodynamic therapy (PDT). Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Expression and chromatin structures of cellulolytic enzyme gene regulated by heterochromatin protein 1.

PubMed

Zhang, Xiujun; Qu, Yinbo; Qin, Yuqi

2016-01-01

Heterochromatin protein 1 (HP1, homologue HepA in Penicillium oxalicum ) binding is associated with a highly compact chromatin state accompanied by gene silencing or repression. HP1 loss leads to the derepression of gene expression. We investigated HepA roles in regulating cellulolytic enzyme gene expression, as an increasingly number of studies have suggested that cellulolytic enzyme gene expression is not only regulated by transcription factors, but is also affected by the chromatin status. Among the genes that exhibited significant differences between the hepA deletion strain (Δ hepA ) and the wild type (WT), most (95.0 %) were upregulated in Δ hepA compared with WT. The expression of the key transcription factor for cellulolytic enzyme gene (e.g., repressor CreA and activator ClrB) increased significantly. However, the deletion of hepA led to downregulation of prominent extracellular cellulolytic enzyme genes. Among the top 10 extracellular glycoside hydrolases (Amy15A, Amy13A, Cel7A/CBHI, Cel61A, Chi18A, Cel3A/BGLI, Xyn10A, Cel7B/EGI, Cel5B/EGII, and Cel6A/CBHII), in which secretion amount is from the highest to the tenth in P . oxalicum secretome, eight genes, including two amylase genes ( amy15A and amy13A ), all five cellulase genes ( cel7A / cbh1 , cel6A / cbh2 , cel7B / eg1 , cel5B / eg2 , and cel3A / bgl1 ), and the cellulose-active LPMO gene ( cel61A ) expression were downregulated. Results of chromatin accessibility real-time PCR (CHART-PCR) showed that the chromatin of all three tested upstream regions opened specifically because of the deletion of hepA in the case of two prominent cellulase genes cel7A/cbh1 and cel7B/eg1 . However, the open chromatin status did not occur along with the activation of cellulolytic enzyme gene expression. The overexpression of hepA upregulated the cellulolytic enzyme gene expression without chromatin modification. The overexpression of hepA remarkably activated the cellulolytic enzyme synthesis, not only in WT (~150 % filter paper activity (FPA) increase), but also in the industry strain RE-10 (~20-30 % FPA increase). HepA is required for chromatin condensation of prominent cellulase genes. However, the opening of chromatin mediated by the deletion of hepA was not positively correlated with cellulolytic enzyme gene activation. HepA is actually a positive regulator for cellulolytic enzyme gene expression and could be a promising target for genetic modification to improve cellulolytic enzyme synthesis.

Assessment of nanoparticle exposure in nanosilica handling process: including characteristics of nanoparticles leaking from a vacuum cleaner.

PubMed

Kim, Boowook; Kim, Hyunwook; Yu, Il Je

2014-01-01

Nanosilica is one of the most widely used nanomaterials across the world. However, their assessment data on the occupational exposure to nanoparticles is insufficient. The present study performed an exposure monitoring in workplace environments where synthetic powders are prepared using fumed nanosilica. Furthermore, after it was observed during exposure monitoring that nanoparticles were emitted through leakage in a vacuum cleaner (even with a HEPA-filter installed in it), the properties of the leaked nanoparticles were also investigated. Workers were exposed to high-concentration nanosilica emitted into the air while pouring it into a container or transferring the container. The use of a vacuum cleaner with a leak (caused by an inadequate sealing) was found to be the origin of nanosilica dispersion in the indoor air. While the particle size of the nanosilica that emitted into the air (during the handling of nanosilica by a worker) was mostly over 100 nm or several microns (µm) due to the coagulation of particles, the size of nanosilica that leaked out of vacuum cleaner was almost similar to the primary size (mode diameter 11.5 nm). Analysis of area samples resulted in 20% (60% in terms of peak concentration) less than the analysis of the personals sample.

Assessment of Nanoparticle Exposure in Nanosilica Handling Process: Including Characteristics of Nanoparticles Leaking from a Vacuum Cleaner

PubMed Central

KIM, Boowook; KIM, Hyunwook; YU, Il Je

2013-01-01

Nanosilica is one of the most widely used nanomaterials across the world. However, their assessment data on the occupational exposure to nanoparticles is insufficient. The present study performed an exposure monitoring in workplace environments where synthetic powders are prepared using fumed nanosilica. Furthermore, after it was observed during exposure monitoring that nanoparticles were emitted through leakage in a vacuum cleaner (even with a HEPA-filter installed in it), the properties of the leaked nanoparticles were also investigated. Workers were exposed to high-concentration nanosilica emitted into the air while pouring it into a container or transferring the container. The use of a vacuum cleaner with a leak (caused by an inadequate sealing) was found to be the origin of nanosilica dispersion in the indoor air. While the particle size of the nanosilica that emitted into the air (during the handling of nanosilica by a worker) was mostly over 100 nm or several microns (µm) due to the coagulation of particles, the size of nanosilica that leaked out of vacuum cleaner was almost similar to the primary size (mode diameter 11.5 nm). Analysis of area samples resulted in 20% (60% in terms of peak concentration) less than the analysis of the personals sample. PMID:24366536

A Quasi-Experimental Study Analyzing the Effectiveness of Portable High-Efficiency Particulate Absorption Filters in Preventing Infections in Hematology Patients during Construction.

PubMed

Özen, Mehmet; Yılmaz, Gülden; Coşkun, Belgin; Topçuoğlu, Pervin; Öztürk, Bengi; Gündüz, Mehmet; Atilla, Erden; Arslan, Önder; Özcan, Muhit; Demirer, Taner; İlhan, Osman; Konuk, Nahide; Balık, İsmail; Gürman, Günhan; Akan, Hamdi

2016-03-05

The increased risk of infection for patients caused by construction and renovation near hematology inpatient clinics is a major concern. The use of high-efficiency particulate absorption (HEPA) filters can reduce the risk of infection. However, there is no standard protocol indicating the use of HEPA filters for patients with hematological malignancies, except for those who have undergone allogeneic hematopoietic stem cell transplantation. This quasi-experimental study was designed to measure the efficacy of HEPA filters in preventing infections during construction. Portable HEPA filters were placed in the rooms of patients undergoing treatment for hematological malignancies because of large-scale construction taking place near the hematology clinic. The rates of infection during the 6 months before and after the installation of the portable HEPA filters were compared. A total of 413 patients were treated during this 1-year period. There were no significant differences in the antifungal prophylaxis and treatment regimens between the groups. The rates of infections, clinically documented infections, and invasive fungal infections decreased in all of the patients following the installation of the HEPA filters. When analyzed separately, the rates of invasive fungal infections were similar before and after the installation of HEPA filters in patients who had no neutropenia or long neutropenia duration. HEPA filters were significantly protective against infection when installed in the rooms of patients with acute lymphocytic leukemia, patients who were undergoing consolidation treatment, and patients who were neutropenic for 1-14 days. Despite the advent of construction and the summer season, during which environmental Aspergillus contamination is more prevalent, no patient or patient subgroup experienced an increase in fungal infections following the installation of HEPA filters. The protective effect of HEPA filters against infection was more pronounced in patients with acute lymphocytic leukemia, patients undergoing consolidation therapy, and patients with moderate neutropenia.

Hepatic Tissue Environment in NEMO-Deficient Mice Critically Regulates Positive Selection of Donor Cells after Hepatocyte Transplantation

PubMed Central

Kaldenbach, Michaela; Cubero, Francisco Javier; Erschfeld, Stephanie; Liedtke, Christian; Trautwein, Christian; Streetz, Konrad

2014-01-01

Background Hepatocyte transplantation (HT) is a promising alternative treatment strategy for end-stage liver diseases compared with orthotopic liver transplantation. A limitation for this approach is the low engraftment of donor cells. The deletion of the I-kappa B kinase-regulatory subunit IKKγ/NEMO in hepatocytes prevents nuclear factor (NF)-kB activation and triggers spontaneous liver apoptosis, chronic hepatitis and the development of liver fibrosis and hepatocellular carcinoma. We hypothesized that NEMOΔhepa mice may therefore serve as an experimental model to study HT. Methods Pre-conditioned NEMOΔhepa mice were transplanted with donor-hepatocytes from wildtype (WT) and mice deficient for the pro-apoptotic mediator Caspase-8 (Casp8Δhepa). Results Transplantation of isolated WT-hepatocytes into pre-conditioned NEMOΔhepa mice resulted in a 6-7 fold increase of donor cells 12 weeks after HT, while WT-recipients showed no liver repopulation. The use of apoptosis-resistant Casp8Δhepa-derived donor cells further enhanced the selection 3-fold after 12-weeks and up to 10-fold increase after 52 weeks compared with WT donors. While analysis of NEMOΔhepa mice revealed strong liver injury, HT-recipient NEMOΔhepa mice showed improved liver morphology and decrease in serum transaminases. Concomitant with these findings, the histological examination elicited an improved liver tissue architecture associated with significantly lower levels of apoptosis, decreased proliferation and a lesser amount of liver fibrogenesis. Altogether, our data clearly support the therapeutic benefit of the HT procedure into NEMOΔhepa mice. Conclusion This study demonstrates the feasibility of the NEMOΔhepa mouse as an in vivo tool to study liver repopulation after HT. The improvement of the characteristic phenotype of chronic liver injury in NEMOΔhepa mice after HT suggests the therapeutic potential of HT in liver diseases with a chronic inflammatory phenotype and opens a new door for the applicability of this technique to combat liver disease in the human clinic. PMID:24979756

Power and Thermal Technologies for Air and Space -- Scientific Research Program. Delivery Order 0016: Developing and Processing High Energy Density Polymer Film Dielectrics for High Temperature Air Force Power Electronic Applications

DTIC Science & Technology

2010-01-01

a vacuum controller. A vacuum of < 1 µ torr was achieved with a combination of a turbo pump and a scroll pump system. The sample probing is...the polymer was reprecipitated in heptane non-solvent. The filtered polymer was washed with heptane and was finally dried in vacuum at 100ºC for three...solution was added to a large excess of methanol to precipitate the polymer. After soxhlet extraction with methanol and vacuum drying, the polymer was

Define and Quantify the Physics of Air Flow, Pressure Drop and Aerosol Collection in Nuclear Grade HEPA Filters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moore, Murray E.

Objective: Develop a set of peer-review and verified analytical methods to adjust HEPA filter performance to different flow rates, temperatures and altitudes. Experimental testing will measure HEPA filter flow rate, pressure drop and efficiency to verify the analytical approach. Nuclear facilities utilize HEPA (High Efficiency Particulate Air) filters to purify air flow for workspace ventilation. However, the ASME AG-1 technical standard (Code on Nuclear Air and Gas Treatment) does not adequately describe air flow measurement units for HEPA filter systems. Specifically, the AG-1 standard does not differentiate between volumetric air flow in ACFM (actual cubic feet per minute)compared to massmore » flow measured in SCFM (standard cubic feet per minute). More importantly, the AG-1 standard has an overall deficiency for using HEPA filter devices at different air flow rates, temperatures, and altitudes. Technical Approach: The collection efficiency and pressure drops of 18 different HEPA filters will be measured over a range of flow rates, temperatures and altitudes. The experimental results will be compared to analytical scoping calculations. Three manufacturers have allocated six HEPA filters each for this effort. The 18 filters will be tested at two different flow rates, two different temperatures and two different altitudes. The 36 total tests will be conducted at two different facilities: the ATI Test facilities (Baltimore MD) and the Los Alamos National Laboratory (Los Alamos NM). The Radiation Protection RP-SVS group at Los Alamos has an aerosol wind tunnel that was originally designed to evaluate small air samplers. In 2010, modifications were started to convert the wind tunnel for HEPA filter testing. (Extensive changes were necessary for the required aerosol generators, HEPA test fixtures, temperature control devices and measurement capabilities.) To this date, none of these modification activities have been funded through a specific DOE or NNSA program. This is expected to require six months of time, after receipt of funding. Benefits: US DOE facilities that use HEPA filters will benefit from access to the new operational measurement methods. Uncertainty and guesswork will be removed from HEPA filter operations.« less

Filtered cathodic arc deposition apparatus and method

DOEpatents

Krauss, Alan R.

1999-01-01

A filtered cathodic arc deposition method and apparatus for the production of highly dense, wear resistant coatings which are free from macro particles. The filtered cathodic arc deposition apparatus includes a cross shaped vacuum chamber which houses a cathode target having an evaporable surface comprised of the coating material, means for generating a stream of plasma, means for generating a transverse magnetic field, and a macro particle deflector. The transverse magnetic field bends the generated stream of plasma in the direction of a substrate. Macro particles are effectively filtered from the stream of plasma by traveling, unaffected by the transverse magnetic field, along the initial path of the plasma stream to a macro particle deflector. The macro particle deflector has a preformed surface which deflects macro particles away from the substrate.

Volatility and leachability of heavy metals and radionuclides in thermally treated HEPA filter media generated from nuclear facilities.

PubMed

Yoon, In-Ho; Choi, Wang-Kyu; Lee, Suk-Chol; Min, Byung-Youn; Yang, Hee-Chul; Lee, Kune-Woo

2012-06-15

The purpose of the present study was to apply thermal treatments to reduce the volume of HEPA filter media and to investigate the volatility and leachability of heavy metals and radionuclides during thermal treatment. HEPA filter media were transformed to glassy bulk material by thermal treatment at 900°C for 2h. The most abundant heavy metal in the HEPA filter media was Zn, followed by Sr, Pb and Cr, and the main radionuclide was Cs-137. The volatility tests showed that the heavy metals and radionuclides in radioactive HEPA filter media were not volatilized during the thermal treatment. PCT tests indicated that the leachability of heavy metals and radionuclides was relatively low compared to those of other glasses. XRD results showed that Zn and Cs reacted with HEPA filter media and were transformed into crystalline willemite (ZnO·SiO(2)) and pollucite (Cs(2)OAl(2)O(3)4SiO(2)), which are not volatile or leachable. The proposed technique for the volume reduction and transformation of radioactive HEPA filter media into glassy bulk material is a simple and energy efficient procedure without additives that can be performed at relatively low temperature compared with conventional vitrification process. Copyright © 2012 Elsevier B.V. All rights reserved.

Contamination Impact of Station Brush Fire on Cleanroom Facilities

NASA Technical Reports Server (NTRS)

Carey, Phil; Blakkolb, Brian

2010-01-01

Brush and forest fires, both naturally occurring and anthropogenic in origin, in proximity to space flight hardware processing facilities raise concerns about the threat of contamination resulting from airborne particulate and molecular components of smoke. Perceptions of the severity of the threat are possibly heightened by the high sensitivity of the human sense of smell to some components present in the smoke of burning vegetation.On August 26th, 2009, a brushfire broke out north of Pasadena, California, two miles from the Jet Propulsion Laboratory. The Station Fire destroyed over 160,000 acres, coming within a few hundred yards of JPL. Smoke concentrations on Lab were very heavy over several days. All Lab operations were halted, and measures were taken to protect personnel, critical hardware, and facilities. Evaluation of real-time cleanroom monitoring data, visualinspection of facilities, filter systems, and analysis of surface cleanliness samples revealed facility environments andhardware were minimally effected.Outside air quality easily exceeded Class Ten Million. Prefilters captured most large ash and soot; multi-stage filtration greatly minimized the impact on the HEPA/ULPA filters. Air quality in HEPA filtered spacecraft assembly cleanrooms remained within Class 10,000 specification throughout. Surface cleanliness was inimally affected, as large particles were effectively removed from the airstream, and sub-micron particles have extremely long settling rates. Approximate particulate fallout within facilities was 0.00011% area coverage/day compared to 0.00038% area coverage/day during normal operations. Deposition of condensable airborne components, as measured in real time, peaked at approximately1.0 ng/cm2/day compared to 0.05 ng/cm2/day nominal.

Women's Health-Enhancing Physical Activity and Eudaimonic Well Being

ERIC Educational Resources Information Center

Ferguson, Leah J.; Kowalski, Kent C.; Mack, Diane E.; Wilson, Philip M.; Crocker, Peter R. E.

2012-01-01

In this study, we explored the role of health-enhancing physical activity (HEPA; Miilunpalo, 2001) in women's eudaimonic well being (i.e., psychological flourishing at one's maximal potential; Ryff, 1989). We used a quantitative approach (N = 349) to explore the relationship between HEPA and eudaimonic well being. While HEPA was not related to…

Ceramic High Efficiency Particulate Air (HEPA) Filter Final Report CRADA No. TC02160.0

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mitchell, M.; Bergman, W.

2017-08-25

The technical objective of this project was to develop a ceramic HEPA filter technology, by initially producing and testing coupon ceramics, small scale prototypes, and full scale prototype HEPA filters, and to address relevant manufacturing and commercialization technical issues.

The Design, Synthesis and Screening of Potential Pyridinium Oxime Prodrugs

DTIC Science & Technology

1985-09-30

210 ml stainless steel bombs were each filled with 100 ml of 28% aqueous NH4OH, 2.8 g (11 imol) of copper sulfate pentahydrate , and 15 g (87 mol) of...ethyl acetate. The organic extracts were washed twice with brine, dried over sodium sulfate , filtered and flashed to a black oil. A vacuum distillation...extracts were washed with brine, dried with sodium sulfate , filtered and flashed. The residue was then purified by column chromatography (silica gel

A Quasi-Experimental Study Analyzing the Effectiveness of Portable High-Efficiency Particulate Absorption Filters in Preventing Infections in Hematology Patients during Construction

PubMed Central

Özen, Mehmet; Yılmaz, Gülden; Coşkun, Belgin; Topçuoğlu, Pervin; Öztürk, Bengi; Gündüz, Mehmet; Atilla, Erden; Arslan, Önder; Özcan, Muhit; Demirer, Taner; İlhan, Osman; Konuk, Nahide; Balık, İsmail; Gürman, Günhan; Akan, Hamdi

2016-01-01

Objective: The increased risk of infection for patients caused by construction and renovation near hematology inpatient clinics is a major concern. The use of high-efficiency particulate absorption (HEPA) filters can reduce the risk of infection. However, there is no standard protocol indicating the use of HEPA filters for patients with hematological malignancies, except for those who have undergone allogeneic hematopoietic stem cell transplantation. This quasi-experimental study was designed to measure the efficacy of HEPA filters in preventing infections during construction. Materials and Methods: Portable HEPA filters were placed in the rooms of patients undergoing treatment for hematological malignancies because of large-scale construction taking place near the hematology clinic. The rates of infection during the 6 months before and after the installation of the portable HEPA filters were compared. A total of 413 patients were treated during this 1-year period. Results: There were no significant differences in the antifungal prophylaxis and treatment regimens between the groups. The rates of infections, clinically documented infections, and invasive fungal infections decreased in all of the patients following the installation of the HEPA filters. When analyzed separately, the rates of invasive fungal infections were similar before and after the installation of HEPA filters in patients who had no neutropenia or long neutropenia duration. HEPA filters were significantly protective against infection when installed in the rooms of patients with acute lymphocytic leukemia, patients who were undergoing consolidation treatment, and patients who were neutropenic for 1-14 days. Conclusion: Despite the advent of construction and the summer season, during which environmental Aspergillus contamination is more prevalent, no patient or patient subgroup experienced an increase in fungal infections following the installation of HEPA filters. The protective effect of HEPA filters against infection was more pronounced in patients with acute lymphocytic leukemia, patients undergoing consolidation therapy, and patients with moderate neutropenia. PMID:26376622

Hepatic tissue environment in NEMO-deficient mice critically regulates positive selection of donor cells after hepatocyte transplantation.

PubMed

Kaldenbach, Michaela; Cubero, Francisco Javier; Erschfeld, Stephanie; Liedtke, Christian; Trautwein, Christian; Streetz, Konrad

2014-01-01

Hepatocyte transplantation (HT) is a promising alternative treatment strategy for end-stage liver diseases compared with orthotopic liver transplantation. A limitation for this approach is the low engraftment of donor cells. The deletion of the I-kappa B kinase-regulatory subunit IKKγ/NEMO in hepatocytes prevents nuclear factor (NF)-kB activation and triggers spontaneous liver apoptosis, chronic hepatitis and the development of liver fibrosis and hepatocellular carcinoma. We hypothesized that NEMOΔhepa mice may therefore serve as an experimental model to study HT. Pre-conditioned NEMOΔhepa mice were transplanted with donor-hepatocytes from wildtype (WT) and mice deficient for the pro-apoptotic mediator Caspase-8 (Casp8Δhepa). Transplantation of isolated WT-hepatocytes into pre-conditioned NEMOΔhepa mice resulted in a 6-7 fold increase of donor cells 12 weeks after HT, while WT-recipients showed no liver repopulation. The use of apoptosis-resistant Casp8Δhepa-derived donor cells further enhanced the selection 3-fold after 12-weeks and up to 10-fold increase after 52 weeks compared with WT donors. While analysis of NEMOΔhepa mice revealed strong liver injury, HT-recipient NEMOΔhepa mice showed improved liver morphology and decrease in serum transaminases. Concomitant with these findings, the histological examination elicited an improved liver tissue architecture associated with significantly lower levels of apoptosis, decreased proliferation and a lesser amount of liver fibrogenesis. Altogether, our data clearly support the therapeutic benefit of the HT procedure into NEMOΔhepa mice. This study demonstrates the feasibility of the NEMOΔhepa mouse as an in vivo tool to study liver repopulation after HT. The improvement of the characteristic phenotype of chronic liver injury in NEMOΔhepa mice after HT suggests the therapeutic potential of HT in liver diseases with a chronic inflammatory phenotype and opens a new door for the applicability of this technique to combat liver disease in the human clinic.

Hepatocyte specific deletion of c-Met leads to the development of severe non-alcoholic steatohepatitis in mice.

PubMed

Kroy, Daniela C; Schumacher, Fabienne; Ramadori, Pierluigi; Hatting, Maximilian; Bergheim, Ina; Gassler, Nikolaus; Boekschoten, Mark V; Müller, Michael; Streetz, Konrad L; Trautwein, Christian

2014-10-01

Non-alcoholic-fatty-liver disease (NAFLD) is part of the metabolic syndrome. The spectrum of NAFLD includes NASH (non-alcoholic steatohepatitis), which is characterised by progressive inflammation associated with oxidative stress and apoptosis, finally triggering liver cirrhosis and hepatocellular carcinoma. HGF (hepatocyte growth factor)/mesenchymal-epithelial transition factor (c-Met) receptor signalling is known to activate distinct intracellular pathways mediating among others anti-apoptotic properties to hepatocytes. Therefore, the aim was to characterise the role of c-Met during NASH development. Hepatocyte specific c-Met knockout mice (c-MetΔ(hepa)) using the cre-loxP system and wild type controls (c-Met(loxP/loxP)) were fed a methionine-choline deficient (MCD) diet. MCD feeding triggered massive steatosis, decreased survival and higher transaminases in c-MetΔ(hepa) livers compared to c-Met(loxP/loxP). Gene array analysis demonstrated that genes involved in fatty acid metabolism were strongly upregulated in c-MetΔ(hepa) livers correlating with higher amounts of hepatic free fatty acids. Consequently, c-MetΔ(hepa) mice showed significantly more TUNEL positive cells and more superoxide anion production than c-Met(loxPloxP) animals. Additionally, c-MetΔ(hepa) livers showed significantly larger fractions of infiltrating neutrophils, macrophages, and cytotoxic T cells. These changes correlated with an enhanced progression of liver fibrosis as evidenced by higher collagen deposition in c-MetΔ(hepa) livers. As increased apoptosis was a prominent feature in c-MetΔ(hepa) livers, we generated c-Met/Casp8Δ(hepa) double knockout mice. In these animals compared to c-MetΔ(hepa) animals the increase in apoptosis could be reverted. c-Met deletion in hepatocytes triggers NASH progression. A prominent mechanism is higher fatty acid accumulation and increased apoptosis, which in part can be reverted by blocking caspase 8. Copyright © 2014 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

HEPA filter dissolution process

DOEpatents

Brewer, K.N.; Murphy, J.A.

1994-02-22

A process is described for dissolution of spent high efficiency particulate air (HEPA) filters and then combining the complexed filter solution with other radioactive wastes prior to calcining the mixed and blended waste feed. The process is an alternate to a prior method of acid leaching the spent filters which is an inefficient method of treating spent HEPA filters for disposal. 4 figures.

NIOSH comments to DOL on the Occupational Safety and Health Administration's proposed rule on occupational exposure to asbestos, tremolite, anthophyllite, and actinolite by J. D. Millar, April 26, 1991

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1991-04-26

The testimony contains the comments of NIOSH regarding the proposed rule on occupational exposure to asbestos (1332214), tremolite (14567738), anthophyllite (17068789), and actinolite (77536664). The testimony included a memorandum comparing NIOSH Method 7400 to OSHA Method ID 160, an evaluation of glove bag containment in asbestos removal, the limit of detection that was ascertained from the sampling strategies for three sets of samples, effectiveness of periodic cleaning of surfaces with a HEPA vacuum, an update on cancer mortality among chrysotile (12001295) asbestos miners, and the use of hand tools to remove built up roofing.

Hepatitis A vaccination coverage among adults 18–49 years traveling to a country of high or intermediate endemicity, United States

PubMed Central

Lu, Peng-jun; Byrd, Kathy K.; Murphy, Trudy V.

2018-01-01

Background Since 1996, hepatitis A vaccine (HepA) has been recommended for adults at increased risk for infection including travelers to high or intermediate hepatitis A endemic countries. In 2009, travel outside the United States and Canada was the most common exposure nationally reported for persons with hepatitis A virus (HAV) infection. Objective To assess HepA vaccination coverage among adults 18–49 years traveling to a country of high or intermediate endemicity in the United States. Methods We analyzed data from the 2010 National Health Interview Survey (NHIS), to determine self-reported HepA vaccination coverage (≥1 dose) and series completion (≥2 dose) among persons 18–49 years who traveled, since 1995, to a country of high or intermediate HAV endemicity. Multivariable logistic regression and predictive marginal analyses were conducted to identify factors independently associated with HepA vaccine receipt. Results In 2010, approximately 36.6% of adults 18–49 years reported traveling to high or intermediate hepatitis A endemic countries; among this group unadjusted HepA vaccination coverage was 26.6% compared to 12.7% among non-travelers (P-values < 0.001) and series completion were 16.9% and 7.6%, respectively (P-values < 0.001). On multivariable analysis among all respondents, travel status was an independent predictor of HepA coverage and series completion (both P-values < 0.001). Among travelers, HepA coverage and series completion (≥2 doses) were higher for travelers 18–25 years (prevalence ratios 2.3, 2.8, respectively, P-values < 0.001) and for travelers 26–39 years (prevalence ratios 1.5, 1.5, respectively, P-value < 0.001, P-value = 0.002, respectively) compared to travelers 40–49 years. Other characteristics independently associated with a higher likelihood of HepA receipt among travelers included Asian race/ethnicity, male sex, never having been married, having a high school or higher education, living in the western United States, having greater number of physician contacts or receipt of influenza vaccination in the previous year. HepB vaccination was excluded from the model because of the significant correlation between receipt of HepA vaccination and HepB vaccination could distort the model. Conclusions Although travel to a country of high or intermediate hepatitis A endemicity was associated with higher likelihood of HepA vaccination in 2010 among adults 18–49 years, self-reported HepA vaccination coverage was low among adult travelers to these areas. Healthcare providers should ask their patients’ upcoming travel plans and recommend and offer travel related vaccinations to their patients. PMID:23523408

Hepa filter dissolution process

DOEpatents

Brewer, Ken N.; Murphy, James A.

1994-01-01

A process for dissolution of spent high efficiency particulate air (HEPA) filters and then combining the complexed filter solution with other radioactive wastes prior to calcining the mixed and blended waste feed. The process is an alternate to a prior method of acid leaching the spent filters which is an inefficient method of treating spent HEPA filters for disposal.

Mold Occurring on the Air Cleaner High-Efficiency Particulate Air Filters Used in the Houses of Child Patients with Atopic Dermatitis

PubMed Central

Kim, Seong Hwan; Ahn, Geum Ran; Son, Seung Yeol; Bae, Gwi-Nam

2014-01-01

Fungi are the known sources of irritation associated with atopic diseases (e.g., asthma, allergic rhinoconjunctivitis, and atopic eczema). To quantitatively estimate their presence in the indoor environment of atopic dermatitis-inflicted child patient's houses (ADCPHs), the high-efficiency particulate air (HEPA) filters installed inside the air cleaners of three different ADCPHs were investigated for the presence of mold. The air cleaner HEPA filters obtained from the three different ADCPHs were coded as HEPA-A, -B, and -C, respectively, and tested for the presence of mold. The colony forming units (CFUs) corresponding to the HEPA-A, -B, and -C filters were estimated to be 6.51 × 102 ± 1.50 × 102 CFU/cm2, 8.72 × 102 ± 1.69 × 102 CFU/cm2, and 9.71 × 102 ± 1.35 × 102 CFU/cm2, respectively. Aspergillus, Penicillium, Alternaria, Cladosporium, Trichoderma, and other fungal groups were detected in the 2,494 isolates. The distribution of these fungal groups differed among the three filters. Cladosporium was the major fungal group in filters HEPA-A and -C, whereas Penicillium was the major fungal group in the filter HEPA-B. Nine fungal species, including some of the known allergenic species, were identified in these isolates. Cladosporium cladosporioides was the most common mold among all the three filters. This is the first report on the presence of fungi in the air cleaner HEPA filters from ADCPHs in Korea. PMID:25346608

Analysis of an MCU HEPA filter

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peters, T. B.; Fondeur, F. F.

A series of direct analyses on three portions (inlet, center, and outlet) of the High Efficiency Particulate Air (HEPA) filter material from the Modular Caustic-Side Solvent Extraction Unit (MCU) have been performed; this includes x-ray methods such as X-Ray Diffraction (XRD), Contained Scanning Electron Microscopy (CSEM) and X-Ray Fluorescence (XRF), as well as Fourier Transform InfraRed spectroscopy (FTIR). Additionally, two leaching studies (one with water, one with dichloromethane) have been performed on three portions (inlet, center, and outlet) of the HEPA filter material, with the leachates being analyzed by Inductively-coupled plasma emission spectroscopy (ICPES), Semi-Volatile Organic Analysis (SVOA) and gammascan.more » From the results of the analyses, SRNL feels that cesium-depleted solvent is being introduced into the HEPA filter. The most likely avenue for this is mechanical aerosolization of solvent, where the aerosol is then carried along an airstream into the HEPA filter. Once introduced into the HEPA filter media, the solvent wicks throughout the material, and migrates towards the outlet end. Once on the outlet end, continual drying could cause particulate flakes to exit the filter and travel farther down the airstream path.« less

Water washable stainless steel HEPA filter

DOEpatents

Phillips, Terrance D.

2001-01-01

The invention is a high efficiency particulate (HEPA) filter apparatus and system, and method for assaying particulates. The HEPA filter provides for capture of 99.99% or greater of particulates from a gas stream, with collection of particulates on the surface of the filter media. The invention provides a filter system that can be cleaned and regenerated in situ.

Preparation and investigation of diamond-like carbon stripper foils by filtered cathodic vacuum arc

NASA Astrophysics Data System (ADS)

Fan, Qiwen; Du, Yinghui; Zhang, Rong; Xu, Guoji

2013-04-01

Thin diamond-like carbon (DLC) stripper foils ˜5 μg/cm2 in thickness were produced and evaluated as heavy-ion strippers for the Beijing HI-13 Tandem Accelerator. The DLC layers ˜4 μg/cm2 in thickness were produced by the filtered cathodic vacuum arc technology onto glass slides coated with betaine-saccharose as releasing agent, which were previously covered with evaporated carbon layers ˜1 μg/cm2 in thickness by the controlled ac arc-discharge method. Irradiation lifetimes of the DLC stripper foils were tested using the heavy-ion beams at the terminal of the Beijing HI-13 Tandem Accelerator, and compared with those of the standard carbon stripper foils made by the combined dc and ac arc-discharge method. The measurements indicate that the DLC stripper foils outlast the standard combined dc and ac arc-discharge carbon stripper foils by a factor of at least 13 and 4for the 197Au- (˜9 MeV, ˜1 μA) and 63Cu- (˜9 MeV, ˜1 μA) ion beams, respectively. The structure and properties of the DLC foils deposited onto silicon substrates by the filtered cathodic vacuum arc technology were also evaluated and analyzed by scanning electron microscopy, Raman spectroscopy and X-ray photoelectron spectroscopy. The scanning electron microscopy images show that the DLC foils contain hardly droplets through the double 90° filters. The X-ray photoelectron spectrum indicates that sp3 bonds of the DLC foils exceed 70%. The integral intensity ratio of the D peak to the G peak (ID/IG) measured by the Raman spectroscopy is0.78.

Nanodiamond embedded ta-C composite film by pulsed filtered vacuum arc deposition from a single target

NASA Astrophysics Data System (ADS)

Iyer, Ajai; Etula, Jarkko; Ge, Yanling; Liu, Xuwen; Koskinen, Jari

2016-11-01

Detonation Nanodiamonds (DNDs) are known to have sp3 core, sp2 shell, small size (few nm) and are gaining importance as multi-functional nanoparticles. Diverse methods have been used to form composites, containing detonation nanodiamonds (DNDs) embedded in conductive and dielectric matrices for various applications. Here we show a method, wherein DND-ta-C composite film, consisting of DNDs embedded in ta-C matrix have been co-deposited from the same cathode by pulsed filtered cathodic vacuum arc method. Transmission Electron Microscope analysis of these films revel the presence of DNDs embedded in the matrix of amorphous carbon. Raman spectroscopy indicates that the presence of DNDs does not adversely affect the sp3 content of DND-ta-C composite film compared to ta-C film of same thickness. Nanoindentation and nanowear tests indicate that DND-ta-C composite films possess improved mechanical properties in comparison to ta-C films of similar thickness.

Contamination impact of station brush fire on cleanroom facilities

NASA Astrophysics Data System (ADS)

Carey, Philip A.; Blakkolb, Brian K.

2010-08-01

Brush and forest fires, both naturally occurring and anthropogenic in origin, in proximity to space flight hardware processing facilities raise concerns about the threat of contamination resulting from airborne particulate and molecular components of smoke. Perceptions of the severity of the threat are possibly heightened by the high sensitivity of the human sense of smell to some components present in the smoke of burning vegetation. On August 26th, 2009, a brushfire broke out north of Pasadena, California, two miles from the Jet Propulsion Laboratory. The Station Fire destroyed over 160,000 acres, coming within a few hundred yards of JPL. Smoke concentrations on Lab were very heavy over several days. All Lab operations were halted, and measures were taken to protect personnel, critical hardware, and facilities. Evaluation of real-time cleanroom monitoring data, visual inspection of facilities, filter systems, and analysis of surface cleanliness samples revealed facility environments and hardware were minimally effected. Outside air quality easily exceeded Class Ten Million. Prefilters captured most large ash and soot; multi-stage filtration greatly minimized the impact on the HEPA/ULPA filters. Air quality in HEPA filtered spacecraft assembly cleanrooms remained within Class 10,000 specification throughout. Surface cleanliness was minimally affected, as large particles were effectively removed from the airstream, and sub-micron particles have extremely long settling rates. Approximate particulate fallout within facilities was 0.00011% area coverage/day compared to 0.00038% area coverage/day during normal operations. Deposition of condensable airborne components, as measured in real time, peaked at approximately 1.0 ng/cm2/day compared to 0.05 ng/cm2/day nominal.

Pseudo ribbon metal ion beam source.

PubMed

Stepanov, Igor B; Ryabchikov, Alexander I; Sivin, Denis O; Verigin, Dan A

2014-02-01

The paper describes high broad metal ion source based on dc macroparticle filtered vacuum arc plasma generation with the dc ion-beam extraction. The possibility of formation of pseudo ribbon beam of metal ions with the parameters: ion beam length 0.6 m, ion current up to 0.2 A, accelerating voltage 40 kV, and ion energy up to 160 kV has been demonstrated. The pseudo ribbon ion beam is formed from dc vacuum arc plasma. The results of investigation of the vacuum arc evaporator ion-emission properties are presented. The influence of magnetic field strength near the cathode surface on the arc spot movement and ion-emission properties of vacuum-arc discharge for different cathode materials are determined. It was shown that vacuum-arc discharge stability can be reached when the magnetic field strength ranges from 40 to 70 G on the cathode surface.

Identifying Strategies Programs Adopt to Meet Healthy Eating and Physical Activity Standards in Afterschool Programs.

PubMed

Weaver, Robert G; Moore, Justin B; Turner-McGrievy, Brie; Saunders, Ruth; Beighle, Aaron; Khan, M Mahmud; Chandler, Jessica; Brazendale, Keith; Randell, Allison; Webster, Collin; Beets, Michael W

2017-08-01

The YMCA of USA has adopted Healthy Eating and Physical Activity (HEPA) Standards for its afterschool programs (ASPs). Little is known about strategies YMCA ASPs are implementing to achieve Standards and these strategies' effectiveness. (1) Identify strategies implemented in YMCA ASPs and (2) evaluate the relationship between strategy implementation and meeting Standards. HEPA was measured via accelerometer (moderate-to-vigorous-physical-activity [MVPA]) and direct observation (snacks served) in 20 ASPs. Strategies were identified and mapped onto a capacity building framework ( Strategies To Enhance Practice [STEPs]). Mixed-effects regression estimated increases in HEPA outcomes as implementation increased. Model-implied estimates were calculated for high (i.e., highest implementation score achieved), moderate (median implementation score across programs), and low (lowest implementation score achieved) implementation for both HEPA separately. Programs implemented a variety of strategies identified in STEPs. For every 1-point increase in implementation score 1.45% (95% confidence interval = 0.33% to 2.55%, p ≤ .001) more girls accumulated 30 min/day of MVPA and fruits and/or vegetables were served on 0.11 more days (95% confidence interval = 0.11-0.45, p ≤ .01). Relationships between implementation and other HEPA outcomes did not reach statistical significance. Still regression estimates indicated that desserts are served on 1.94 fewer days (i.e., 0.40 vs. 2.34) in the highest implementing program than the lowest implementing program and water is served 0.73 more days (i.e., 2.37 vs. 1.64). Adopting HEPA Standards at the national level does not lead to changes in routine practice in all programs. Practical strategies that programs could adopt to more fully comply with the HEPA Standards are identified.

Hepatitis A vaccination coverage among adults 18-49 years traveling to a country of high or intermediate endemicity, United States.

PubMed

Lu, Peng-Jun; Byrd, Kathy K; Murphy, Trudy V

2013-05-01

Since 1996, hepatitis A vaccine (HepA) has been recommended for adults at increased risk for infection including travelers to high or intermediate hepatitis A endemic countries. In 2009, travel outside the United States and Canada was the most common exposure nationally reported for persons with hepatitis A virus (HAV) infection. To assess HepA vaccination coverage among adults 18-49 years traveling to a country of high or intermediate endemicity in the United States. We analyzed data from the 2010 National Health Interview Survey (NHIS), to determine self-reported HepA vaccination coverage (≥1 dose) and series completion (≥2 dose) among persons 18-49 years who traveled, since 1995, to a country of high or intermediate HAV endemicity. Multivariable logistic regression and predictive marginal analyses were conducted to identify factors independently associated with HepA vaccine receipt. In 2010, approximately 36.6% of adults 18-49 years reported traveling to high or intermediate hepatitis A endemic countries; among this group unadjusted HepA vaccination coverage was 26.6% compared to 12.7% among non-travelers (P-values<0.001) and series completion were 16.9% and 7.6%, respectively (P-values<0.001). On multivariable analysis among all respondents, travel status was an independent predictor of HepA coverage and series completion (both P-values<0.001). Among travelers, HepA coverage and series completion (≥2 doses) were higher for travelers 18-25 years (prevalence ratios 2.3, 2.8, respectively, P-values<0.001) and for travelers 26-39 years (prevalence ratios 1.5, 1.5, respectively, P-value<0.001, P-value=0.002, respectively) compared to travelers 40-49 years. Other characteristics independently associated with a higher likelihood of HepA receipt among travelers included Asian race/ethnicity, male sex, never having been married, having a high school or higher education, living in the western United States, having greater number of physician contacts or receipt of influenza vaccination in the previous year. HepB vaccination was excluded from the model because of the significant correlation between receipt of HepA vaccination and HepB vaccination could distort the model. Although travel to a country of high or intermediate hepatitis A endemicity was associated with higher likelihood of HepA vaccination in 2010 among adults 18-49 years, self-reported HepA vaccination coverage was low among adult travelers to these areas. Healthcare providers should ask their patients' upcoming travel plans and recommend and offer travel related vaccinations to their patients. Published by Elsevier Ltd.

Mold occurring on the air cleaner high-efficiency particulate air filters used in the houses of child patients with atopic dermatitis.

PubMed

Kim, Seong Hwan; Ahn, Geum Ran; Son, Seung Yeol; Bae, Gwi-Nam; Yun, Yeo Hong

2014-09-01

Fungi are the known sources of irritation associated with atopic diseases (e.g., asthma, allergic rhinoconjunctivitis, and atopic eczema). To quantitatively estimate their presence in the indoor environment of atopic dermatitis-inflicted child patient's houses (ADCPHs), the high-efficiency particulate air (HEPA) filters installed inside the air cleaners of three different ADCPHs were investigated for the presence of mold. The air cleaner HEPA filters obtained from the three different ADCPHs were coded as HEPA-A, -B, and -C, respectively, and tested for the presence of mold. The colony forming units (CFUs) corresponding to the HEPA-A, -B, and -C filters were estimated to be 6.51 × 10(2) ± 1.50 × 10(2) CFU/cm(2), 8.72 × 10(2) ± 1.69 × 10(2) CFU/cm(2), and 9.71 × 10(2) ± 1.35 × 10(2) CFU/cm(2), respectively. Aspergillus, Penicillium, Alternaria, Cladosporium, Trichoderma, and other fungal groups were detected in the 2,494 isolates. The distribution of these fungal groups differed among the three filters. Cladosporium was the major fungal group in filters HEPA-A and -C, whereas Penicillium was the major fungal group in the filter HEPA-B. Nine fungal species, including some of the known allergenic species, were identified in these isolates. Cladosporium cladosporioides was the most common mold among all the three filters. This is the first report on the presence of fungi in the air cleaner HEPA filters from ADCPHs in Korea.

HEPA air filter (image)

MedlinePlus

... pet dander and other irritating allergens from the air. Along with other methods to reduce allergens, such ... controlling the amount of allergens circulating in the air. HEPA filters can be found in most air ...

Allergy-Proof Your House

MedlinePlus

... small-particle or high-efficiency particulate air (HEPA) filter. Shampoo the carpet frequently. Curtains and blinds. Use ... dander they shed. Air filtration. Choose an air filter that has a small-particle or HEPA filter. ...

Evaluation of systems for reducing the transmission of Porcine reproductive and respiratory syndrome virus by aerosol

PubMed Central

2006-01-01

Abstract The purpose of this study was to compare 3 methods for the reduction of aerosol transmission of Porcine reproductive and respiratory syndrome virus (PRRSV): high-efficiency particulate air (HEPA) filtration, low-cost filtration, and ultraviolet light (UV) irradiation. The HEPA-filtration system involved a pre-filter screen, a bag filter (EU8 rating), and a HEPA filter (EU13 rating). The low-cost-filtration system contained mosquito netting (pre-filter), a fiberglass furnace filter, and an electrostatic furnace filter. For UV irradiation, a lamp emitted UVC radiation at 253.7 nm. No form of intervention was used in the control group. The experimental facilities consisted of 2 chambers connected by a 1.3-m-long duct. Recipient pigs, housed in chamber 2, were exposed to artificial aerosols created by a mechanically operated mister containing modified live PRRSV vaccine located in chamber 1. Aerosol transmission of PRRSV occurred in 9 of the 10 control replicates, 8 of the 10 UVC-irradiation replicates, 4 of the 10 low-cost-filtration replicates, and 0 of the 10 HEPA-filtration replicates. When compared with no intervention, HEPA filtration and low-cost filtration significantly reduced PRRSV transmission (P < 0.0005 and = 0.0286, respectively), whereas UV irradiation had no effect (P = 0.5). However, low-cost filtration and UV irradiation were significantly less effective (P = 0.043 and P < 0.0005, respectively) than HEPA filtration. In conclusion, under the conditions of this study, HEPA filtration was significantly more effective at reducing aerosol transmission of PRRSV than the other methods evaluated. PMID:16548329

Nor-ursodeoxycholic acid reverses hepatocyte-specific nemo-dependent steatohepatitis.

PubMed

Beraza, Naiara; Ofner-Ziegenfuss, Lisa; Ehedego, Haksier; Boekschoten, Mark; Bischoff, Stephan C; Mueller, Michael; Trauner, Michael; Trautwein, Christian

2011-03-01

Hepatocyte-specific NEMO/NF-κB deleted mice (NEMO(Δhepa)) develop spontaneous non-alcoholic steatohepatitis (NASH). Free fatty acids and bile acids promote DR5 expression. TRAIL/NK cell-mediated activation of TRAIL-R2/DR5 plays an important role during acute injury in NEMO(Δhepa) mice. To inhibit the progression of NASH in the absence of hepatocyte-NEMO/NF-kB signaling. NEMOf/f and NEMO(Δhepa) mice were fed with a low-fat diet, and with two anticholestatic diets; UDCA and NorUDCA. The impact of these treatments on the progression of NASH was evaluated. We show that high expression of DR5 in livers from NEMO(Δhepa) mice is accompanied by an abundant presence of bile acids (BAs), misregulation of BA transporters and significant alteration of lipid metabolism-related genes. Additionally, mice lacking NEMO in hepatocytes spontaneously showed ductular response at young age. Unexpectedly, feeding of NEMO(Δhepa) mice with low-fat diet failed to improve chronic liver injury. Conversely, anti-cholestatic treatment with nor-ursodeoxycholic acid (NorUDCA), but not with ursodeoxycholic acid (UDCA), led to a significant attenuation of liver damage in NEMO(Δhepa) mice. The strong therapeutic effect of NorUDCA relied on a significant downregulation of LXR-dependent lipogenesis and the normalisation of BA metabolism through mechanisms involving cross-talk between Cyp7a1 and SHP. This was associated with the significant improvement of liver histology, NEMO(Δhepa)/NorUDCA-treated mice showed lower apoptosis and reduced CyclinD1 expression, indicating attenuation of the compensatory proliferative response to hepatocellular damage. Finally, fibrosis and ductular reaction markers were significantly reduced in NorUDCA-treated NEMO(Δhepa) mice. Overall, our work demonstrates the contribution of bile acids metabolism to the progression of NASH in the absence of hepatocyte-NF-kB through mechanisms involving DR5-apoptosis, inflammation and fibrosis. Our work suggests a potential therapeutic effect of NorUDCA in attenuating the progression of NASH.

Combined use of an electrostatic precipitator and a high-efficiency particulate air filter in building ventilation systems: Effects on cardiorespiratory health indicators in healthy adults.

PubMed

Day, D B; Xiang, J; Mo, J; Clyde, M A; Weschler, C J; Li, F; Gong, J; Chung, M; Zhang, Y; Zhang, J

2018-05-01

High-efficiency particulate air (HEPA) filtration in combination with an electrostatic precipitator (ESP) can be a cost-effective approach to reducing indoor particulate exposure, but ESPs produce ozone. The health effect of combined ESP-HEPA filtration has not been examined. We conducted an intervention study in 89 volunteers. At baseline, the air-handling units of offices and residences for all subjects were comprised of coarse, ESP, and HEPA filtration. During the 5-week long intervention, the subjects were split into 2 groups, 1 with just the ESP removed and the other with both the ESP and HEPA removed. Each subject was measured for cardiopulmonary risk indicators once at baseline, twice during the intervention, and once 2 weeks after baseline conditions were restored. Measured indoor and outdoor PM 2.5 and ozone concentrations, coupled with time-activity data, were used to calculate exposures. Removal of HEPA filters increased 24-hour mean PM 2.5 exposure by 38 (95% CI: 31, 45) μg/m 3 . Removal of ESPs decreased 24-hour mean ozone exposure by 2.2 (2.0, 2.5) ppb. No biomarkers were significantly associated with HEPA filter removal. In contrast, ESP removal was associated with a -16.1% (-21.5%, -10.4%) change in plasma-soluble P-selectin and a -3.0% (-5.1%, -0.8%) change in systolic blood pressure, suggesting reduced cardiovascular risks. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Tribological and Electrical Properties of Diamond-Like Carbon Films Deposited by Filtered Vacuum Arc Method for Medical Guidewire Application.

PubMed

Kang, Ki-Noh; Jeong, Hyejeong; Lee, Jaehyeong; Park, Yong Seob

2018-09-01

A good medical guidewires are used to introduce stents, catheters, and other medical devices inside the human body. In this study, diamond-like carbon (DLC) film was proposed to solve the poor adhesion problem of guidewire and to improve the tribological performance of guidewire. DLC films were fabricated on Si substrate by using FVA (Filtered Vacuum Arc) method. In this work, the tribological, structural, and electrical properties of the fabricated DLC films with various arc currents were experimentally investigated. All DLC films showed smooth and uniform surface with increasing applied arc current. The rms surface roughness was increased and the value of contact angle on the film surface was decreased with increasing arc current. The hardness and elastic modulus of DLC films were improved, and the resistivity value of DLC films were decreased with increasing arc current. These results are associated with ion bombardment effects by the applied arc current and bias voltage.

Incomplete lung recovery following sub-acute inhalation of combustion-derived ultrafine particles in mice.

PubMed

Noël, A; Xiao, R; Perveen, Z; Zaman, H M; Rouse, R L; Paulsen, D B; Penn, A L

2016-02-24

Particulate matter (PM) is one of the six criteria pollutant classes for which National Ambient Air Quality Standards have been set by the United States Environmental Protection Agency. Exposures to PM have been correlated with increased cardio-pulmonary morbidity and mortality. Butadiene soot (BDS), generated from the incomplete combustion of 1,3-butadiene (BD), is both a model PM mixture and a real-life example of a petrochemical product of incomplete combustion. There are numerous events, including wildfires, accidents at refineries and tank car explosions that result in sub-acute exposure to high levels of airborne particles, with the people exposed facing serious health problems. These real-life events highlight the need to investigate the health effects induced by short-term exposure to elevated levels of PM, as well as to assess whether, and if so, how well these adverse effects are resolved over time. In the present study, we investigated the extent of recovery of mouse lungs 10 days after inhalation exposures to environmentally-relevant levels of BDS aerosols had ended. Female BALB/c mice exposed to either HEPA-filtered air or to BDS (5 mg/m(3) in HEPA filtered air, 4 h/day, 21 consecutive days) were sacrificed immediately, or 10 days after the final BDS exposure. Bronchoalveolar lavage fluid (BALF) was collected for cytology and cytokine analysis. Lung proteins and RNA were extracted for protein and gene expression analysis. Lung histopathology evaluation also was performed. Sub-acute exposures of mice to hydrocarbon-rich ultrafine particles induced: (1) BALF neutrophil elevation; (2) lung mucosal inflammation, and (3) increased BALF IL-1β concentration; with all three outcomes returning to baseline levels 10 days post-exposure. In contrast, (4) lung connective tissue inflammation persisted 10 days post-exposure; (5) we detected time-dependent up-regulation of biotransformation and oxidative stress genes, with incomplete return to baseline levels; and (6) we observed persistent particle alveolar load following 10 days of recovery. These data show that 10 days after a 21-day exposure to 5 mg/m(3) of BDS has ended, incomplete lung recovery promotes a pro-biotransformation, pro-oxidant, and pro-inflammatory milieu, which may be a starting point for potential long-term cardio-pulmonary effects.

Invasive aspergillosis in severely neutropenic patients over 18 years: impact of intranasal amphotericin B and HEPA filtration.

PubMed

Withington, S; Chambers, S T; Beard, M E; Inder, A; Allen, J R; Ikram, R B; Schousboe, M I; Heaton, D C; Spearing, R I; Hart, D N

1998-01-01

The impact of intranasal amphotericin B and high-efficiency particulate air (HEPA) filtration on the incidence of invasive aspergillosis was reviewed in patients from 1977 to 1994 undergoing intensive chemotherapy. Overall, the incidence of proven invasive aspergillosis was reduced from 24.4% (1977-1984) to 7.1% (1985-1991) (P < 0.001) following the introduction of intranasal prophylaxis, but when probable cases of aspergillosis were included and lymphoma cases excluded, there was no change in incidence. Following the introduction of HEPA filtration, patient exposure to aspergillus spores as measured by air sampling was markedly reduced and there were no new cases of invasive aspergillosis. HEPA filtration proved effective in reducing invasive aspergillosis and has allowed increasingly aggressive treatment regimens to be introduced.

Hepatitis A and Hepatitis B vaccination coverage among adults with chronic liver disease

PubMed Central

Yue, Xin; Black, Carla L.; O’Halloran, Alissa; Lu, Peng-Jun; Williams, Walter W.; Nelson, Noele P.

2018-01-01

Background Infection with hepatitis A and hepatitis B virus can increase the risk of morbidity and mortality in persons with chronic liver disease (CLD). The Advisory Committee on Immunization Practices recommends hepatitis A (HepA) and hepatitis B (HepB) vaccination for persons with CLD. Methods Data from the 2014 and 2015 National Health Interview Surveys (NHIS), nationally representative, in-person interview surveys of the non-institutionalized US civilian population, were used to assess self-reported HepA (≥1 and ≥2 doses) and HepB vaccination (≥1 and ≥3 doses) coverage among adults who reported a chronic or long-term liver condition. Multivariable logistic regression was used to identify factors independently associated with HepA and HepB vaccination among adults with CLD. Results Overall, 19.4% and 11.5% of adults aged ≥18 years with CLD reported receiving ≥1 dose and ≥2 doses of HepA vaccine, respectively, compared with 14.7% and 9.1% of adults without CLD (p<0.05 comparing those with and without CLD, ≥1dose). Age, education, geographic region, and international travel were associated with receipt of ≥2 doses HepA vaccine among adults with CLD. Overall, 35.7% and 29.1% of adults with CLD reported receiving ≥1 dose and ≥3 doses of HepB vaccine, respectively, compared with 30.2% and 24.7% of adults without CLD (p<0.05 comparing those with and without CLD, ≥1 dose). Age, education, and receipt of influenza vaccination in the past 12 months were associated with receipt of ≥3 doses HepB vaccine among adults with CLD. Among adults with CLD and ≥10 provider visits, only 13.8% and 35.3% had received ≥2 doses HepA and ≥3 doses HepB vaccine, respectively. Conclusions HepA and HepB vaccination among adults with CLD is suboptimal and missed opportunities to vaccinate occurred. Providers should adhere to recommendations to vaccinate persons with CLD to increase vaccination among this population. PMID:29395521

Stay away from asthma triggers

MedlinePlus

... cleaner with a HEPA (high-efficiency particulate arrestor) filter. Replace wall-to-wall carpet with wood or ... a central air conditioning system, use a HEPA filter to remove pet allergens from indoor air. Use ...

The Air Pollution Exposure Laboratory (APEL) for controlled human exposure to diesel exhaust and other inhalants: characterization and comparison to existing facilities.

PubMed

Birger, Nicholas; Gould, Timothy; Stewart, James; Miller, Mark R; Larson, Timothy; Carlsten, Chris

2011-03-01

The Air Pollution Exposure Laboratory (APEL) was designed for the controlled inhalation of human subjects to aged and diluted diesel exhaust (DE) to mimic "real-world" occupational and environmental conditions. An EPA Tier 3-compliant, 6.0 kW diesel generator is operated under discrete cyclic loads to simulate diesel on-road emissions. The engine accepts standard ultra-low sulfur diesel or a variety of alternative fuels (such as biodiesel) via a partitioned tank. A portion of raw exhaust is drawn into the primary dilution system and is diluted 9:1 with compressed air at standard temperature (20°C) and humidity (40%) levels. The exhaust is further diluted approximately 25:1 by high efficiency particulate air (HEPA)-filtered air (FA) and then aged for 4 min before entering the 4 × 6 × 7-foot exposure booth. An optional HEPA filter path immediately proximal to the booth can generate a particle-reduced (gas-enriched) exposure. In-booth particulate is read by a nephelometer to provide an instantaneous light scattering coefficient for closed-loop system control. A Scanning Mobility Particle Sizer and multi-stage impactor measures particle size distribution. Filter sampling allows determination of sessional average concentrations of size-fractionated and unfractionated particulate oxidative potential, elemental carbon, organic carbon and trace elements. Approximately 300 μg/m(3) PM(2.5) is routinely achievable at APEL and is well characterized in terms of oxidative potential and elemental components. APEL efficiently creates fresh DE, appropriately aged and diluted for human experimentation at safe yet realistic concentrations. Description of exposure characteristics allows comparison to other international efforts to deepen the current evidence base regarding the health effects of DE.

Turning the tide: national policy approaches to increasing physical activity in seven European countries.

PubMed

Bull, Fiona; Milton, Karen; Kahlmeier, Sonja; Arlotti, Alberto; Juričan, Andrea Backović; Belander, Olov; Martin, Brian; Martin-Diener, Eva; Marques, Ana; Mota, Jorge; Vasankari, Tommi; Vlasveld, Anita

2015-06-01

Physical inactivity is one of the four leading behavioural risk factors for non-communicable disease (NCD). Like tobacco control, increasing levels of health-enhancing physical activity (HEPA) will require a national policy framework providing direction and a clear set of actions. Despite frequent calls, there has been insufficient progress on policy development in the majority of countries around the world. This study sought and summarised national HEPA policy in seven European countries (Finland, Italy, the Netherlands, Norway, Portugal, Slovenia and Switzerland). Data collection used a policy audit tool (PAT), a 27-item instrument structured into four sections. All countries reported some legislation or policy across the sectors of education, sport and health. Only some countries reported supportive policy in the transport and environment sectors. Five countries reported a stand-alone HEPA policy and six countries reported national recommendations. HEPA prevalence targets varied in magnitude and specificity and the presence of other relevant goals from different sectors highlighted the opportunity for joint action. Evaluation and the use of scientific evidence were endorsed but described as weak in practice. Only two countries reported a national multisector coordinating committee and most countries reported challenges with partnerships on different levels of policy implementation. Bringing together the key components for success within a national HEPA policy framework is not simple. This in-depth policy audit and country comparison highlighted similarities and differences and revealed new opportunities for consideration by other countries. These examples can inform countries within and beyond Europe and guide the development of national HEPA policy within the NCD prevention agenda. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

29 CFR 1910.134 - Respiratory protection.

Code of Federal Regulations, 2014 CFR

2014-07-01

... impact and penetration. High efficiency particulate air (HEPA) filter means a filter that is at least 99... as a high efficiency particulate air (HEPA) filter, or an air-purifying respirator equipped with a... frequency of respirator use (including use for rescue and escape); (C) The expected physical work effort; (D...

Health hazards associated with the use of di-(2-ethylhexyl) phthalate (commonly referred to as DOP) in HEPA filter test

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-01-01

Di-(2-ethylhexyl) phthalate (DEHP), commonly referred to as di-octyl phthalate, is an important production chemical in the US. In addition to its major use as an additive in plastics, DEHP is widely used to evaluate the effectiveness of high efficiency particulate air (HEPA) filters. Historically, DEHP was also used in quantitative fit testing for respirators. Evaluations of this compound a decade ago showed that it can induce hepatocellular carcinomas in laboratory animals. Although most Department of Energy (DOE) facilities have since discontinued using DEHP in respirator fit testing, DEHP continues to be used for evaluating HEPA filters. This report summarizes availablemore » information on the toxicity, mutagenicity, carcinogenicity, and other hazards and problems posed by DEHP, specifically with reference to HEPA filter testing. Information on work practice improvements as well as the availability and suitability of DEHP substitutes are also presented. This material should assist the DOE in the safe use of this material.« less

The impact of onsite workplace health-enhancing physical activity interventions on worker productivity: a systematic review.

PubMed

Pereira, Michelle Jessica; Coombes, Brooke Kaye; Comans, Tracy Anne; Johnston, Venerina

2015-06-01

The aim of this study is to investigate the effects of onsite workplace health-enhancing physical activity (HEPA) programmes on worker productivity. The PROSPERO registration number is CRD42014008750. A search for controlled trials or randomised controlled trials (RCTs) that investigated the effects of onsite workplace HEPA programmes on productivity levels of working adults was performed. Risk of bias of included studies was assessed, and the inter-rater reliability of the quality assessment was analysed. Qualitative synthesis of available evidence is presented. Eight studies were included in the review. There is consistent evidence that onsite workplace HEPA programmes do not reduce levels of sick leave. There appears to be inconsistent evidence of the impact of onsite workplace HEPA programmes on worker productivity. A high-quality study of an onsite combination (aerobic, strengthening and flexibility) HEPA regime and a moderate-quality study of a Tai Chi programme improved worker productivity measured with questionnaires in female laundry workers and older female nurses, respectively. Two high-quality studies and four moderate-quality studies did not show benefit. Studies that showed benefit were mainly those designed with productivity measures as primary outcomes, delivered to occupations involved with higher physical loads, and had higher compliance and programme intensity. The small number of studies and the lack of consistency among studies limited further analyses. There is inconsistent evidence that onsite workplace HEPA programmes improve self-reported worker productivity. Future high-quality RCTs of onsite workplace HEPA programmes should be designed around productivity outcomes, target at-risk groups and investigate interventions of sufficient intensity. High attendance with improved recording is needed to achieve significant results in augmenting worker productivity. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Hepatitis A and hepatitis B vaccination coverage among adults with chronic liver disease.

PubMed

Yue, Xin; Black, Carla L; O'Halloran, Alissa; Lu, Peng-Jun; Williams, Walter W; Nelson, Noele P

2018-02-21

Infection with hepatitis A and hepatitis B virus can increase the risk of morbidity and mortality in persons with chronic liver disease (CLD). The Advisory Committee on Immunization Practices recommends hepatitis A (HepA) and hepatitis B (HepB) vaccination for persons with CLD. Data from the 2014 and 2015 National Health Interview Surveys (NHIS), nationally representative, in-person interview surveys of the non-institutionalized US civilian population, were used to assess self-reported HepA (≥1 and ≥2 doses) and HepB vaccination (≥1 and ≥3 doses) coverage among adults who reported a chronic or long-term liver condition. Multivariable logistic regression was used to identify factors independently associated with HepA and HepB vaccination among adults with CLD. Overall, 19.4% and 11.5% of adults aged ≥ 18 years with CLD reported receiving ≥1 dose and ≥2 doses of HepA vaccine, respectively, compared with 14.7% and 9.1% of adults without CLD (p < .05 comparing those with and without CLD, ≥1dose). Age, education, geographic region, and international travel were associated with receipt of ≥2 doses HepA vaccine among adults with CLD. Overall, 35.7% and 29.1% of adults with CLD reported receiving ≥1 dose and ≥3 doses of HepB vaccine, respectively, compared with 30.2% and 24.7% of adults without CLD (p < .05 comparing those with and without CLD, ≥1 dose). Age, education, and receipt of influenza vaccination in the past 12 months were associated with receipt of ≥3 doses HepB vaccine among adults with CLD. Among adults with CLD and ≥10 provider visits, only 13.8% and 35.3% had received ≥2 doses HepA and ≥3 doses HepB vaccine, respectively. HepA and HepB vaccination among adults with CLD is suboptimal and missed opportunities to vaccinate occurred. Providers should adhere to recommendations to vaccinate persons with CLD to increase vaccination among this population. Copyright © 2018 Elsevier Ltd. All rights reserved.

40 CFR 721.10077 - 3H-1,2,4-Triazol-3-one, 1,2-dihydro-.

Code of Federal Regulations, 2013 CFR

2013-07-01

...-fitting respirator equipped with N100 (if aerosols absent), R100, or P100 filters (either half- or full... Efficiency Particulate Air (HEPA) filters; powered air-purifying respirator equipped with a tight-fitting facepiece (either half- or full-face) and HEPA filters; and supplied-air respirator operated in pressure...

40 CFR 721.10411 - Alkanenitrile, bis(cyanoalkyl)amino (generic) (P-07-537).

Code of Federal Regulations, 2014 CFR

2014-07-01

... with N100 (if oil aerosols absent), R100, or P100 filters; NIOSH-certified powered air-purifying respirator equipped with a loose- fitting hood or helmet and high efficiency particulate air (HEPA) filters... HEPA filters; or NIOSH-certified supplied-air respirator operated in pressure demand or continuous flow...

24 CFR 35.140 - Prohibited methods of paint removal.

Code of Federal Regulations, 2013 CFR

2013-04-01

... air (HEPA) local exhaust control. (c) Abrasive blasting or sandblasting without HEPA local exhaust control. (d) Heat guns operating above 1100 degrees Fahrenheit or charring the paint. (e) Dry sanding or dry scraping, except dry scraping in conjunction with heat guns or within 1.0 ft. (0.30 m.) of...

40 CFR 721.10411 - Alkanenitrile, bis(cyanoalkyl)amino (generic) (P-07-537).

Code of Federal Regulations, 2013 CFR

2013-07-01

... with N100 (if oil aerosols absent), R100, or P100 filters; NIOSH-certified powered air-purifying respirator equipped with a loose- fitting hood or helmet and high efficiency particulate air (HEPA) filters... HEPA filters; or NIOSH-certified supplied-air respirator operated in pressure demand or continuous flow...

The influence of extraction procedure on ion concentrations in sediment pore water

USGS Publications Warehouse

Winger, P.V.; Lasier, P.J.; Jackson, B.P.

1998-01-01

Sediment pore water has the potential to yield important information on sediment quality, but the influence of isolation procedures on the chemistry and toxicity are not completely known and consensus on methods used for the isolation from sediment has not been reached. To provide additional insight into the influence of collection procedures on pore water chemistry, anion (filtered only) and cation concentrations were measured in filtered and unfiltered pore water isolated from four sediments using three different procedures: dialysis, centrifugation and vacuum. Peepers were constructed using 24-cell culture plates and cellulose membranes, and vacuum extractors consisted of fused-glass air stones attached with airline tubing to 60cc syringes. Centrifugation was accomplished at two speeds (2,500 and 10,000 x g) for 30 min in a refrigerated centrifuge maintained at 4?C. Only minor differences in chemical characteristics and cation and anion concentrations were found among the different collecting methods with differences being sediment specific. Filtering of the pore water did not appreciably reduce major cation concentrations, but trace metals (Cu and Pb) were markedly reduced. Although the extraction methods evaluated produced pore waters of similar chemistries, the vacuum extractor provided the following advantages over the other methods: (1) ease of extraction, (2) volumes of pore water isolated, (3) minimal preparation time and (4) least time required for extraction of pore water from multiple samples at one time.

Strategies to Increase After-School Program Staff Skills to Promote Healthy Eating and Physical Activity.

PubMed

Weaver, R Glenn; Beets, Michael W; Beighle, Aaron; Webster, Collin; Huberty, Jennifer; Moore, Justin B

2016-01-01

Standards targeting children's healthy eating and physical activity (HEPA) in after-school programs call for staff to display or refrain from HEPA-promoting or -discouraging behaviors that are linked to children's HEPA. This study evaluated strategies to align staff behaviors with HEPA Standards. Staff at four after-school programs serving approximately 500 children participated in professional development training from January 2012 to May 2013. Site leaders also attended workshops and received technical support during the same time frame. Changes in staff behaviors were evaluated using the System for Observing Staff Promotion of Activity and Nutrition in a pre- (fall 2011) multiple-post (spring 2012, fall 2012, and spring 2013), no-control group study design. A total of 8,949 scans were completed across the four measurement periods. Of the 19 behaviors measured, 14 changed in the appropriate direction. For example, staff engaging in physical activity with children increased from 27% to 40% of scans and staff eating unhealthy foods decreased from 56% to 14% of days. Ongoing training and technical assistance can have a measureable impact on staff behaviors linked to child-level HEPA outcomes. Future research should explore the feasibility of disseminating ongoing trainings to after-school program staff on a large scale. © 2015 Society for Public Health Education.

Applied patent RFID systems for building reacting HEPA air ventilation system in hospital operation rooms.

PubMed

Lin, Jesun; Pai, Jar-Yuan; Chen, Chih-Cheng

2012-12-01

RFID technology, an automatic identification and data capture technology to provide identification, tracing, security and so on, was widely applied to healthcare industry in these years. Employing HEPA ventilation system in hospital is a way to ensure healthful indoor air quality to protect patients and healthcare workers against hospital-acquired infections. However, the system consumes lots of electricity which cost a lot. This study aims to apply the RFID technology to offer a unique medical staff and patient identification, and reacting HEPA air ventilation system in order to reduce the cost, save energy and prevent the prevalence of hospital-acquired infection. The system, reacting HEPA air ventilation system, contains RFID tags (for medical staffs and patients), sensor, and reacting system which receives the information regarding the number of medical staff and the status of the surgery, and controls the air volume of the HEPA air ventilation system accordingly. A pilot program was carried out in a unit of operation rooms of a medical center with 1,500 beds located in central Taiwan from Jan to Aug 2010. The results found the air ventilation system was able to function much more efficiently with less energy consumed. Furthermore, the indoor air quality could still keep qualified and hospital-acquired infection or other occupational diseases could be prevented.

29 CFR 1910.1027 - Cadmium.

Code of Federal Regulations, 2010 CFR

2010-07-01

... respirators when they experience eye irritation. (C) Provide HEPA filters for powered and non-powered air.... High-efficiency particulate air (HEPA) filter means a filter capable of trapping and retaining at least..., the employer shall sample the employee(s) expected to have the highest cadmium exposures. (2) Specific...

29 CFR 1910.134 - Respiratory protection.

Code of Federal Regulations, 2012 CFR

2012-07-01

... impact and penetration. High efficiency particulate air (HEPA) filter means a filter that is at least 99... as a high efficiency particulate air (HEPA) filter, or an air-purifying respirator equipped with a... frequency of respirator use (including use for rescue and escape); (C) The expected physical work effort; (D...

29 CFR 1910.134 - Respiratory protection.

Code of Federal Regulations, 2013 CFR

2013-07-01

... impact and penetration. High efficiency particulate air (HEPA) filter means a filter that is at least 99... as a high efficiency particulate air (HEPA) filter, or an air-purifying respirator equipped with a... frequency of respirator use (including use for rescue and escape); (C) The expected physical work effort; (D...

A Custom Robotic System for Inspecting HEPA Filters in the Payload Changeout Room at the NASA Kennedy Space Center

NASA Technical Reports Server (NTRS)

Spencer, James E., Jr.; Looney, Joe

1994-01-01

In this paper, the prime objective is to describe a custom 4-dof (degree-of-freedom) robotic arm capable of autonomously or telerobotically performing systematic HEPA filter inspection and certification in the Shuttle Launch Pad Payload Changeout Rooms (PCR's) on pads A and B at the Kennedy Space Center, Florida. This HEPA filter inspection robot (HFIR) has been designed to be easily deployable and is equipped with the necessary sensory devices, control hardware, software and man-machine interfaces needed to implement HEPA filter inspection reliably and efficiently without damaging the filters or colliding with existing PCR structures or filters. The main purpose of the HFIR is to implement an automated positioning system to move special inspection sensors in pre-defined or manual patterns for the purpose of verifying filter integrity and efficiency. This will ultimately relieve NASA Payload Operations from significant problems associated with time, cost and personnel safety, impacts realized during non-automated PCR HFIR filter certification.

40 CFR 721.10077 - 3H-1,2,4-Triazol-3-one, 1,2-dihydro-.

Code of Federal Regulations, 2011 CFR

2011-07-01

...-fitting respirator equipped with N100 (if aerosols absent), R100, or P100 filters (either half- or full... Efficiency Particulate Air (HEPA) filters; powered air-purifying respirator equipped with a tight-fitting facepiece (either half- or full-face) and HEPA filters; and supplied-air respirator operated in pressure...

40 CFR 721.10077 - 3H-1,2,4-Triazol-3-one, 1,2-dihydro-.

Code of Federal Regulations, 2014 CFR

2014-07-01

...-fitting respirator equipped with N100 (if aerosols absent), R100, or P100 filters (either half- or full... Efficiency Particulate Air (HEPA) filters; powered air-purifying respirator equipped with a tight-fitting facepiece (either half- or full-face) and HEPA filters; and supplied-air respirator operated in pressure...

24 CFR 35.140 - Prohibited methods of paint removal.

Code of Federal Regulations, 2011 CFR

2011-04-01

... air (HEPA) local exhaust control. (c) Abrasive blasting or sandblasting without HEPA local exhaust control. (d) Heat guns operating above 1100 degrees Fahrenheit or charring the paint. (e) Dry sanding or dry scraping, except dry scraping in conjunction with heat guns or within 1.0 ft. (0.30 m.) of...

24 CFR 35.140 - Prohibited methods of paint removal.

Code of Federal Regulations, 2012 CFR

2012-04-01

... air (HEPA) local exhaust control. (c) Abrasive blasting or sandblasting without HEPA local exhaust control. (d) Heat guns operating above 1100 degrees Fahrenheit or charring the paint. (e) Dry sanding or dry scraping, except dry scraping in conjunction with heat guns or within 1.0 ft. (0.30 m.) of...

40 CFR 721.10077 - 3H-1,2,4-Triazol-3-one, 1,2-dihydro-.

Code of Federal Regulations, 2012 CFR

2012-07-01

...-fitting respirator equipped with N100 (if aerosols absent), R100, or P100 filters (either half- or full... Efficiency Particulate Air (HEPA) filters; powered air-purifying respirator equipped with a tight-fitting facepiece (either half- or full-face) and HEPA filters; and supplied-air respirator operated in pressure...

40 CFR 721.10411 - Alkanenitrile, bis(cyanoalkyl)amino (generic) (P-07-537).

Code of Federal Regulations, 2012 CFR

2012-07-01

... with N100 (if oil aerosols absent), R100, or P100 filters; NIOSH-certified powered air-purifying respirator equipped with a loose- fitting hood or helmet and high efficiency particulate air (HEPA) filters... HEPA filters; or NIOSH-certified supplied-air respirator operated in pressure demand or continuous flow...

24 CFR 35.140 - Prohibited methods of paint removal.

Code of Federal Regulations, 2014 CFR

2014-04-01

... air (HEPA) local exhaust control. (c) Abrasive blasting or sandblasting without HEPA local exhaust control. (d) Heat guns operating above 1100 degrees Fahrenheit or charring the paint. (e) Dry sanding or dry scraping, except dry scraping in conjunction with heat guns or within 1.0 ft. (0.30 m.) of...

Further evaluation of alternative air-filtration systems for reducing the transmission of Porcine reproductive and respiratory syndrome virus by aerosol

PubMed Central

Deen, John; Cano, Jean Paul; Batista, Laura; Pijoan, Carlos

2006-01-01

Abstract The purpose of this study was to compare 4 methods for the reduction of aerosol transmission of Porcine reproductive and respiratory syndrome virus (PRRSV): high-efficiency particulate air (HEPA) filtration, 2×-low-cost filtration, bag filtration, and use of a filter tested against particles derived from dioctylphthalate (DOP). The HEPA-filtration system used a prefilter screen, a bag filter (Eurovent [EU] 8 rating), and a HEPA filter (EU13 rating). The low-cost-filtration system contained mosquito netting (prefilter), 2 fiberglass furnace filters, and 2 electrostatic furnace filters. Bag filtration involved the use of a filter rated EU8 and a minimum efficiency reporting value (MERV) of 14. The 95%-DOP, 0.3-μm-filtration system involved a pleat-in-pleat V-bank disposable filter with a 95% efficiency rating for particles 0.3 μm or greater in diameter and ratings of EU9 and MERV 15. No form of intervention was used in the control group. The experimental facilities consisted of 2 chambers connected by a 1.3-m-long duct containing the treatments. Recipient pigs, housed in chamber 2, were exposed to artificial aerosols created by a mechanically operated mister containing modified live PRRSV vaccine located in chamber 1. Aerosol transmission of PRRSV occurred in 0 of the 10 HEPA-filtration replicates, 2 of the 10 bag-filtration replicates, 4 of the 10 low-cost-filtration replicates, 0 of the 10 95%-DOP, 0.3-μm-filtration replicates, and all 10 of the control replicates. Using a similar approach, we further evaluated the HEPA- and 95%-DOP, 0.3-μm-filtration systems. Infection was not observed in any of the 76 HEPA-filtration replicates but was observed in 2 of the 76 95%-DOP, 0.3-μm replicates and 42 of the 50 control replicates. Although the difference between the 95%-DOP, 0.3-μm and control replicates was significant (P < 0.0005), so was the level of failure of the 95%-DOP, 0.3-μm system (P = 0.02). In conclusion, under the conditions of this study, some methods of air filtration were significantly better than others in reducing aerosol transmission of PRRSV, and HEPA filtration was the only system that completely prevented transmission. PMID:16850938

Phase transitions of doped carbon in CrCN coatings with modified mechanical and tribological properties via filtered cathodic vacuum arc deposition

NASA Astrophysics Data System (ADS)

Guan, J. J.; Wang, H. Q.; Qin, L. Z.; Liao, B.; Liang, H.; Li, B.

2017-04-01

The CrCN coatings were fabricated onto Si (1 1 1) wafers and SUS304 stainless steel plates using filtered cathodic vacuum arc deposition (FCVAD) technique under different flow ratios of N2/C2H2 gas mixture. The morphology, crystalline structure and chemical composition of the coatings were characterized. It was found that the grain size reduce with increasing carbon content, which makes the CrCN coatings refined and smooth. The quasi-one-dimensional carbolite phase was also found in CrN host lattice with C2H2 content ranging from 5% to 20%, and it will be evolved into amorphous carbon and amorphous CNx phases as C2H2 content exceeds 20%. Moreover, we examined the mechanical and tribological properties of the CrCN coatings, and the experimental results confirmed that the friction coefficient of the coatings descend to the lowest value as 0.39 with 30% C2H2 content, due to the graphite (sp2 Csbnd C) phase embed in CrN host lattice; while the chromium carbon (Cr3C2) and diamond (sp3 Csbnd C) phases may give rise to the increase of the coating hardness with the highest value at 23.97 GPa under 20% C2H2 content.

Health-enhancing physical activity and health-related risk in a sample of north Mexican, office-based employees.

PubMed

Gilson, Nicholas David

2007-01-01

Levels of hypertension, obesity and raised total cholesterol are increasing in Mexico. Mexicans employed within sedentary occupations may be particularly at risk of developing one, or a clustering of these health-related risk factors, due to lack of participation in health enhancing physical activity (HEPA). The purpose of this study is to examine: (a) prevalence rates of hypertension, obesity and raised total cholesterol, and (b) the link between these variables and HEPA, in a sample of North Mexican, office-based employees. Methods include an assessment of systolic/diastolic blood pressure, % body fat, total cholesterol and HEPA (7-day self-report) in 47 men (33 +/- 10 years) and 43 women (28 +/- 7 years) from Monterrey, a large industrial city in the Northeast of Mexico. Values were compared against recognised health-related thresholds to determine prevalence rates of individual health-related risk factors, along with clustering of two or more risk factors. Relationships between variables were analysed using Pearson product moment correlation. The results show Men had a high prevalence of obesity (32%) and raised total cholesterol (44%), while women's rates were lower (7% and 10% respectively). More men (59%) than women (17%) demonstrated a clustering of two or more risk factors. HEPA participation was low in both men (9%) and women (16%). A significant relationship was found between men's HEPA and % body fat (r = -0.31; p < 0.05). The high levels of inactivity found in both men and women were cause for concern, suggesting the need for innovative intervention approaches, which aim to integrate physical activity into busy working lives.

Unravelling networks in local public health policymaking in three European countries - a systems analysis.

PubMed

Spitters, Hilde P E M; Lau, Cathrine J; Sandu, Petru; Quanjel, Marcel; Dulf, Diana; Glümer, Charlotte; van Oers, Hans A M; van de Goor, Ien A M

2017-02-03

Facilitating and enhancing interaction between stakeholders involved in the policymaking process to stimulate collaboration and use of evidence, is important to foster the development of effective Health Enhancing Physical Activity (HEPA) policies. Performing an analysis of real-world policymaking processes will help reveal the complexity of a network of stakeholders. Therefore, the main objectives were to unravel the stakeholder network in the policy process by conducting three systems analyses, and to increase insight into the similarities and differences in the policy processes of these European country cases. A systems analysis of the local HEPA policymaking process was performed in three European countries involved in the 'REsearch into POlicy to enhance Physical Activity' (REPOPA) project, resulting in three schematic models showing the main stakeholders and their relationships. The models were used to compare the systems, focusing on implications with respect to collaboration and use of evidence in local HEPA policymaking. Policy documents and relevant webpages were examined and main stakeholders were interviewed. The systems analysis in each country identified the main stakeholders involved and their position and relations in the policymaking process. The Netherlands and Denmark were the most similar and both differed most from Romania, especially at the level of accountability of the local public authorities for local HEPA policymaking. The categories of driving forces underlying the relations between stakeholders were formal relations, informal interaction and knowledge exchange. A systems analysis providing detailed descriptions of positions and relations in the stakeholder network in local level HEPA policymaking is rather unique in this area. The analyses are useful when a need arises for increased interaction, collaboration and use of knowledge between stakeholders in the local HEPA network, as they provide an overview of the stakeholders involved and their mutual relations. This information can be an important starting point to enhance the uptake of evidence and build more effective public health policies.

Submicron and Nanoparticulate Matter Removal by HEPA-Rated Media Filters and Packed Beds of Granular Materials

NASA Technical Reports Server (NTRS)

Perry, J. L.; Agui, J. H.; Vijayakimar, R

2016-01-01

Contaminants generated aboard crewed spacecraft by diverse sources consist of both gaseous chemical contaminants and particulate matter. Both HEPA media filters and packed beds of granular material, such as activated carbon, which are both commonly employed for cabin atmosphere purification purposes have efficacy for removing nanoparticulate contaminants from the cabin atmosphere. The phenomena associated with particulate matter removal by HEPA media filters and packed beds of granular material are reviewed relative to their efficacy for removing fine (less than 2.5 micrometers) and ultrafine (less than 0.01 micrometers) sized particulate matter. Considerations are discussed for using these methods in an appropriate configuration to provide the most effective performance for a broad range of particle sizes including nanoparticulates.

Lack of gp130 expression in hepatocytes attenuates tumor progression in the DEN model.

PubMed

Hatting, M; Spannbauer, M; Peng, J; Al Masaoudi, M; Sellge, G; Nevzorova, Y A; Gassler, N; Liedtke, C; Cubero, F J; Trautwein, C

2015-03-05

Chronic liver inflammation is a crucial event in the development and growth of hepatocellular carcinoma (HCC). Compelling evidence has shown that interleukin-6 (IL-6)/gp130-dependent signaling has a fundamental role in liver carcinogenesis. Thus, in the present study we aimed to investigate the role of gp130 in hepatocytes for the initiation and progression of HCC. Hepatocyte-specific gp130 knockout mice (gp130(Δhepa)) and control animals (gp130(f/f)) were treated with diethylnitrosamine (DEN). The role of gp130 for acute injury (0-144 h post treatment), tumor initiation (24 weeks) and progression (40 weeks) was analyzed. After acute DEN-induced liver injury we observed a reduction in the inflammatory response in gp130(Δhepa) animals as reflected by decreased levels of IL-6 and oncostatin M. The loss of gp130 slightly attenuated the initiation of HCC 24 weeks after DEN treatment. In contrast, 40 weeks after DEN treatment, male and female gp130(Δhepa) mice showed smaller tumors and reduced tumor burden, indicating a role for hepatocyte-specific gp130 expression during HCC progression. Oxidative stress and DNA damage were substantially and similarly increased by DEN in both gp130(f/f) and gp130(Δhepa) animals. However, gp130(Δhepa) livers revealed aberrant STAT5 activation and decreased levels of transforming growth factor-β (TGFβ), pSMAD2/3 and SMAD2, whereas phosphorylation of STAT3 at Tyr705 and Ser727 was absent. Our results indicate that gp130 deletion in hepatocytes reduces progression, but not HCC initiation in the DEN model. Gp130 deletion resulted in STAT3 inhibition but increased STAT5 activation and diminished TGF-dependent signaling. Hence, blocking gp130 in hepatocytes might be an interesting therapeutic target to inhibit the growth of HCC.

Can Vacuum Assisted Venous Drainage be Achieved using a Roller Pump in an Emergency? A Pilot Study using Neonatal Circuitry

PubMed Central

Hill, S. L.; Holt, D. W.

2007-01-01

Abstract: There has been much advancement in perfusion technology over its 50 years of progression. One of these techniques is vacuum-assisted venous drainage (VAVD). Many perfusionists augment venous drainage using VAVD, typically from a wall vacuum source. This study explores alternates to providing VAVD if the wall vacuum fails. In two porcine laboratories, ∼36 in. of 3/16-in. tubing was connected to a sucker return port and placed into the roller head next to the arterial pump. The vacuum was monitored with a DLP pressure monitoring system (Medtronic). This system was connected to small-bore tubing and attached to a stopcock on top of the reservoir. The vacuum was regulated using another stopcock connected to a non-filtered luer lock port on top of the reservoir or by a segment of 3 × 0.25-in.-diameter tubing attached to the vent port with a c-clamp. Vacuum drainage was achieved, ranging from −18 mmHg to −71 mmHg by manipulating the stopcock or c-clamp. Changes in venous drainage were seen by volume fluctuations in the venous reservoir. The vacuum was adjusted to account for dramatic changes. Augmented venous drainage using a roller pump can be achieved successfully during cardiopulmonary bypass (CPB). This method of active drainage can be used in lieu of wall suction or during times of emergency if wall suction fails. PMID:18293812

Assessment of penetration through vacuum cleaners and recommendation of wet cyclone technology.

PubMed

Seo, Youngjin; Han, Taewon

2013-04-01

In many commercial vacuum cleaners, the captured aerosol particles contained in the dust collector may accidentally release from the exhaust filtration owing to leakage or penetration. Vacuum cleaners may cause dust to become airborne by exhausting air that is not completely filtered. This may cause the operator to inhale dust, in turn causing health problems. This study aimed to investigate the dust penetration rates from three commercial vacuum cleaners and suggest the best technique for completely filtering exhaust air using a combination of cyclonic separation and water filtration. The commercial vacuum cleaners were tested inside a custom-built hood, and the exhausted particles were monitored using a sampling probe in conjunction with an aerosol particle sizer Quartzose mineral dusts were added to each vacuum cleaner through the dust transport line. A 2400 L/min wet cyclone was employed as the proposed vacuum cleaner It was designed using Stokes scaling, and its collection characteristics were evaluated using polystyrene latex beads. Surprisingly, the conventional vacuum cleaners failed to capture an overall average of approximately 14% of the particles in the given size range. However, only approximately 3.8% of the collected particles escaped from the vacuum cleaner that used the wet cyclone technology. Thus, the proposed vacuum cleaner should potentially be an effective method for vacuuming household dust. The successful investigation of conventional vacuum cleaners is useful for both manufacturers and users. As an effective vacuum cleaning mechanism, household dust is able to migrate along the thin water, film that forms on the inner walls of the cyclone vacuum cleaner. It collects dust in a small water inflow (3 mL/min), which allows it to capture a higher percentage of contaminants than most of the currently available vacuum cleaners. The significantly low accidental exposure rates achieved by this new vacuum cleaner enable healthy conditions in various environments, including indoors.

Air Cleaning Technologies

PubMed Central

2005-01-01

Executive Summary Objective This health technology policy assessment will answer the following questions: When should in-room air cleaners be used? How effective are in-room air cleaners? Are in-room air cleaners that use combined HEPA and UVGI air cleaning technology more effective than those that use HEPA filtration alone? What is the Plasmacluster ion air purifier in the pandemic influenza preparation plan? The experience of severe acute respiratory syndrome (SARS) locally, nationally, and internationally underscored the importance of administrative, environmental, and personal protective infection control measures in health care facilities. In the aftermath of the SARS crisis, there was a need for a clearer understanding of Ontario’s capacity to manage suspected or confirmed cases of airborne infectious diseases. In so doing, the Walker Commission thought that more attention should be paid to the potential use of new technologies such as in-room air cleaning units. It recommended that the Medical Advisory Secretariat of the Ontario Ministry of Health and Long-Term Care evaluate the appropriate use and effectiveness of such new technologies. Accordingly, the Ontario Health Technology Advisory Committee asked the Medical Advisory Secretariat to review the literature on the effectiveness and utility of in-room air cleaners that use high-efficiency particle air (HEPA) filters and ultraviolet germicidal irradiation (UVGI) air cleaning technology. Additionally, the Ontario Health Technology Advisory Committee prioritized a request from the ministry’s Emergency Management Unit to investigate the possible role of the Plasmacluster ion air purifier manufactured by Sharp Electronics Corporation, in the pandemic influenza preparation plan. Clinical Need Airborne transmission of infectious diseases depends in part on the concentration of breathable infectious pathogens (germs) in room air. Infection control is achieved by a combination of administrative, engineering, and personal protection methods. Engineering methods that are usually carried out by the building’s heating, ventilation, and air conditioning (HVAC) system function to prevent the spread of airborne infectious pathogens by diluting (dilution ventilation) and removing (exhaust ventilation) contaminated air from a room, controlling the direction of airflow and the air flow patterns in a building. However, general wear and tear over time may compromise the HVAC system’s effectiveness to maintain adequate indoor air quality. Likewise, economic issues may curtail the completion of necessary renovations to increase its effectiveness. Therefore, when exposure to airborne infectious pathogens is a risk, the use of an in-room air cleaner to reduce the concentration of airborne pathogens and prevent the spread of airborne infectious diseases has been proposed as an alternative to renovating a HVAC system. Airborne transmission is the spread of infectious pathogens over large distances through the air. Infectious pathogens, which may include fungi, bacteria, and viruses, vary in size and can be dispersed into the air in drops of moisture after coughing or sneezing. Small drops of moisture carrying infectious pathogens are called droplet nuclei. Droplet nuclei are about 1 to 5μm in diameter. This small size in part allows them to remain suspended in the air for several hours and be carried by air currents over considerable distances. Large drops of moisture carrying infectious pathogens are called droplets. Droplets being larger than droplet nuclei, travel shorter distances (about 1 metre) before rapidly falling out of the air to the ground. Because droplet nuclei remain airborne for longer periods than do droplets, they are more amenable to engineering infection control methods than are droplets. Droplet nuclei are responsible for the airborne transmission of infectious diseases such as tuberculosis, chicken pox (varicella), measles (rubeola), and dessiminated herpes zoster, whereas close contact is required for the direct transmission of infectious diseases transmitted by droplets, such as influenza (the flu) and SARS. The Technology In-room air cleaners are supplied as portable or fixed devices. Fixed devices can be attached to either a wall or ceiling and are preferred over portable units because they have a greater degree of reliability (if installed properly) for achieving adequate room air mixing and airflow patterns, which are important for optimal effectiveness. Through a method of air recirculation, an in-room air cleaner can be used to increase room ventilation rates and if used to exhaust air out of the room it can create a negative-pressure room for airborne infection isolation (AII) when the building’s HVAC system cannot do so. A negative-pressure room is one where clean air flows into the room but contaminated air does not flow out of it. Contaminated room air is pulled into the in-room air cleaner and cleaned by passing through a series of filters, which remove the airborne infectious pathogens. The cleaned air is either recirculated into the room or exhausted outside the building. By filtering contaminated room air and then recirculating the cleaned air into the room, an in-room air cleaner can improve the room’s ventilation. By exhausting the filtered air to the outside the unit can create a negative-pressure room. There are many types of in-room air cleaners. They vary widely in the airflow rates through the unit, the type of air cleaning technology used, and the technical design. Crucial to maximizing the efficiency of any in-room air cleaner is its strategic placement and set-up within a room, which should be done in consultation with ventilation engineers, infection control experts, and/or industrial hygienists. A poorly positioned air cleaner may disrupt airflow patterns within the room and through the air cleaner, thereby compromising its air cleaning efficiency. The effectiveness of an in-room air cleaner to remove airborne pathogens from room air depends on several factors, including the airflow rate through the unit’s filter and the airflow patterns in the room. Tested under a variety of conditions, in-room air cleaners, including portable or ceiling mounted units with either a HEPA or a non-HEPA filter, portable units with UVGI lights only, or ceiling mounted units with combined HEPA filtration and UVGI lights, have been estimated to be between 30% and 90%, 99% and 12% and 80% effective, respectively. However, and although their effectiveness is variable, the United States Centers for Disease Control and Prevention has acknowledged in-room air cleaners as alternative technology for increasing room ventilation when this cannot be achieved by the building’s HVAC system with preference given to fixed recirculating systems over portable ones. Importantly, the use of an in-room air cleaner does not preclude either the need for health care workers and visitors to use personal protective equipment (N95 mask or equivalent) when entering AII rooms or health care facilities from meeting current regulatory requirements for airflow rates (ventilation rates) in buildings and airflow differentials for effective negative-pressure rooms. The Plasmacluster ion technology, developed in 2000, is an air purification technology. Its manufacturer, Sharp Electronics Corporation, says that it can disable airborne microorganisms through the generation of both positive and negative ions. (1) The functional unit is the hydroxyl, which is a molecule comprised of one oxygen molecule and one hydrogen atom. Plasmacluster ion air purifier uses a multilayer filter system composed of a prefilter, a carbon filter, an antibacterial filter, and a HEPA filter, combined with an ion generator to purify the air. The ion generator uses an alternating plasma discharge to split water molecules into positively and negatively charged ions. When these ions are emitted into the air, they are surrounded by water molecules and form cluster ions which are attracted to airborne particles. The cluster ion surrounds the airborne particle, and the positive and negative ions react to form hydroxyls. These hydroxyls steal the airborne particle’s hydrogen atom, which creates a hole in the particle’s outer protein membrane, thereby rendering it inactive. Because influenza is primarily acquired by large droplets and direct and indirect contact with an infectious person, any in-room air cleaner will have little benefit in controlling and preventing its spread. Therefore, there is no role for the Plasmacluster ion air purifier or any other in-room air cleaner in the control of the spread of influenza. Accordingly, for purposes of this review, the Medical Advisory Secretariat presents no further analysis of the Plasmacluster. Review Strategy The objective of the systematic review was to determine the effectiveness of in-room air cleaners with built in UVGI lights and HEPA filtration compared with those using HEPA filtration only. The Medical Advisory Secretariat searched the databases of MEDLINE, EMBASE, Cochrane Database of Systematic Reviews, INAHATA (International Network of Agencies for Health Technology Assessment), Biosis Previews, Bacteriology Abstracts, Web of Science, Dissertation Abstracts, and NIOSHTIC 2. A meta-analysis was conducted if adequate data was available from 2 or more studies and where statistical and clinical heterogeneity among studies was not an issue. Otherwise, a qualitative review was completed. The GRADE system was used to summarize the quality of the body of evidence comprised of 1 or more studies. Summary of Findings There were no existing health technology assessments on air cleaning technology located during the literature review. The literature search yielded 59 citations of which none were retained. One study was retrieved from a reference list of a guidance document from the United States Centers for Disease Control and Prevention, which evaluated an in-room air cleaner with combined UVGI lights and HEPA filtration under 2 conditions: UVGI lights on and UVGI lights off. Experiments were performed using different ventilation rates and using an aerosolized pathogen comprised of Mycobaterium parafortuitum, a surrogate for the bacterium that causes tuberculosis. Effectiveness was measured as equivalent air changes per hour (eACH). This single study formed the body of evidence for our systematic review research question. Experimental Results The eACH rate for the HEPA-UVGI in-room air cleaner was statistically significantly greater when the UV lights were on compared with when the UV lights were off. (P < .05). However, subsequent experiments could not attribute this to the UVGI. Consequently, the results are inconclusive and an estimate of effect (benefit) is uncertain. The study was reviewed by a scientific expert and rated moderate for quality. Further analysis determined that there was some uncertainty in the directness of the outcome measure (eACH); thus, the GRADE level for the quality of the evidence was low indicating that an estimate of effect is very uncertain. There is uncertainty in the benefits of using in-room air cleaners with combined UVGI lights and HEPA filtration over systems that use HEPA filtration alone. However, there are no known risks to using systems with combined UVGI and HEPA technology compared with those with HEPA alone. There is an increase in the burden of cost including capital costs (cost of the device), operating costs (electricity usage), and maintenance costs (cleaning and replacement of UVGI lights) to using an in-room air cleaner with combined UVGI and HEPA technology compared with those with HEPA alone. Given the uncertainty of the estimate of benefits, an in-room air cleaner with HEPA technology only may be an equally reasonable alternative to using one with combined UVGI and HEPA technology Conclusions In-room air cleaners may be used to protect health care staff from air borne infectious pathogens such as tuberculosis, chicken pox, measles, and dessiminated herpes zoster. In addition, and although in-room air cleaners are not effective at protecting staff and preventing the spread of droplet-transmitted diseases such as influenza and SARS, they may be deployed in situations with a novel/emerging infectious agent whose epidemiology is not yet defined and where airborne transmission is suspected. It is preferable that in-room air cleaners be used with a fixed and permanent room placement when ventilation requirements must be improved and the HVAC system cannot be used. However, for acute (temporary) situations where a novel/emerging infectious agent presents whose epidemiology is not yet defined and where airborne transmission is suspected it may be prudent to use the in room air cleaner as a portable device until mode of transmission is confirmed. To maximize effectiveness, consultation with an environmental engineer and infection control expert should be undertaken before using an in-room air cleaner and protocols for maintenance and monitoring of these devices should be in place. If properly installed and maintained, in room air cleaners with HEPA or combined HEPA and UVGI air cleaning technology are effective in removing airborne pathogens. However, there is only weak evidence available at this time regarding the benefit of using an in-room air cleaner with combined HEPA and UVGI air cleaner technology instead of those with HEPA filter technology only. PMID:23074468

Survey of HEPA filter experience

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carbaugh, E.H.

1982-07-01

A survey of high efficiency particulate air (HEPA) filter applications and experience at Department of Energy (DOE) sites was conducted to provide an overview of the reasons and magnitude of HEPA filter changeouts and failures. Results indicated that approximately 58% of the filters surveyed were changed out in the three year study period, and some 18% of all filters were changed out more than once. Most changeouts (63%) were due to the existence of a high pressure drop across the filter, indicative of filter plugging. Other reasons for changeout included leak-test failure (15%), preventive maintenance service life limit (13%), suspectedmore » damage (5%) and radiation buildup (4%). Filter failures occurred with approximately 12% of all installed filters. Of these failures, most (64%) occurred for unknown or unreported reasons. Handling or installation damage accounted for an additional 19% of reported failures. Media ruptures, filter-frame failures and seal failures each accounted for approximately 5 to 6% of the reported failures.« less

Institute for Clean Energy Technology Mississippi State University NSR&D Aged HEPA Filter Study Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jacks, Robert; Stormo, Julie; Rose, Coralie

Data have demonstrated that filter media lose tensile strength and the ability to resist the effects of moisture as a function of age. Testing of new and aged filters needs to be conducted to correlate reduction of physical strength of HEPA media to the ability of filters to withstand upset conditions. Appendix C of the Nuclear Air Cleaning Handbook provides the basis for DOE’s HEPA filter service life guidance. However, this appendix also points out the variability of data, and it does not correlate performance of aged filters to degradation of media due to age. Funding awarded by NSR&D tomore » initiate full-scale testing of aged HEPA filters addresses the issue of correlating media degradation due to age with testing of new and aged HEPA filters under a generic design basis event set of conditions. This funding has accelerated the process of describing this study via: (1) establishment of a Technical Working Group of all stakeholders, (2) development and approval of a test plan, (3) development of testing and autopsy procedures, (4) acquiring an initial set of aged filters, (5) testing the initial set of aged filters, and (6) developing the filter test report content for each filter tested. This funding was very timely and has moved the project forward by at least three years. Activities have been correlated with testing conducted under DOE-EM funding for evaluating performance envelopes for AG-1 Section FC Separator and Separatorless filters. This coordination allows correlation of results from the NSR&D Aged Filter Study with results from testing new filters of the Separator and Separatorless Filter Study. DOE-EM efforts have identified approximately 100 more filters of various ages that have been stored under Level B conditions. NSR&D funded work allows a time for rigorous review among subject matter experts before moving forward with development of the testing matrix that will be used for additional filters. The NSR&D data sets are extremely valuable in as much as establishing a selfimproving, NQA-1 program capable of advancing the service lifetime study of HEPA filters. The data and reports are available for careful and critical review by subject matter experts before the next set of filters is tested and can be found in the appendices of this final report. NSR&D funds have not only initiated the Aged HEPA Filter Study alluded to in Appendix C of the NACH, but have also enhanced the technical integrity and effectiveness of all of the follow-on testing for this long-term study.« less

Comparison of aryl hydrocarbon hydroxylase and acetanilide 4-hydroxylase induction by polycyclic aromatic compounds in human and mouse cell lines.

PubMed

Jaiswal, A K; Nebert, D W; Eisen, H W

1985-08-01

The human MCF-7 and the mouse Hepa-1 cell culture lines were compared for aryl hydrocarbon hydroxylase and acetanilide 4-hydroxylase inducibility by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and benzo[a]anthracene (BA) and TCDD- and BA-specific binding in the cytosol and nucleus. The effective concentration of BA in the growth medium required to induce either enzyme to 50% of its maximally inducible activity (EC50) was the same (5-11 microM) in both MCF-7 and Hepa-1 cells. On the other hand, the EC50 for TCDD in MCF-7 cells (5-25 nM) was more than 40-fold greater than that in Hepa-1 cells (0.4 to 0.6 nM). P1-450- and P3-450-specific mouse cDNA probes were used to quantitate mRNA induction in the Hepa-1 cell line. P1-450 mRNA was induced markedly by TCDD and benzo[a] anthracene, whereas P3-450 mRNA was induced negligibly. A P1-450-specific human cDNA probe was used to quantitate P1-450 mRNA induction in the MCF-7 cell line. Aryl hydrocarbon hydroxylase inducibility by TCDD or BA always paralleled P1-450 mRNA inducibility in either the mouse or human line. Although the cytosolic Ah receptor in Hepa-1 cells was easily detected by sucrose density gradient centrifugation, gel permeation chromatography, and anion-exchange high-performance liquid chromatography, the cytosolic receptor cannot be detected in MCF-7 cells. Following in vivo exposure of cultures to radiolabeled TCDD, the intranuclear concentration of inducer-receptor complex was at least fifty times greater in Hepa-1 than MCF-7 cultures. The complete lack of measurable cytosolic receptor and almost totally absent inducer-receptor complex in the nucleus of MCF-7 cells was, therefore, out of proportion to its capacity for aryl hydrocarbon hydroxylase and acetanilide 4-hydroxylase inducibility. This MCF-7 line should provide an interesting model for a better understanding of the mechanisms of drug-metabolizing enzyme induction by polycyclic aromatic compounds, including the Ah receptor-mediated mechanism.

Influence of argon and oxygen on charge-state-resolved ion energydistributions of filtered aluminum arcs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rosen, Johanna; Anders, Andre; Mraz, Stanislav

2006-03-23

The charge-state-resolved ion energy distributions (IEDs) in filtered aluminum vacuum arc plasmas were measured and analyzed at different oxygen and argon pressures in the range 0.5 8.0 mTorr. A significant reduction of the ion energy was detected as the pressure was increased, most pronounced in an argon environment and for the higher charge states. The corresponding average charge state decreased from 1.87 to 1.0 with increasing pressure. The IEDs of all metal ions in oxygen were fitted with shifted Maxwellian distributions. The results show that it is possible to obtain a plasma composition with a narrow charge-state distribution as wellmore » as a narrow IED. These data may enable tailoring thin-film properties through selecting growth conditions that are characterized by predefined charge state and energy distributions.« less

Reliability and Validity of the SE-HEPA: Examining Physical Activity- and Healthy Eating-Specific Self-Efficacy among a Sample of Preadolescents

ERIC Educational Resources Information Center

Steele, Michael M.; Burns, Leonard G.; Whitaker, Brandi N.

2013-01-01

Objective. The purpose of this study was to examine the psychometric properties of the self-efficacy for healthy eating and physical activity measure (SE-HEPA) for preadolescents. Method. The reliability of the measure was examined to determine if the internal consistency of the measure was adequate (i.e., [alpha]s greater than 0.70). Next, in an…

Comparison of MERV 16 and HEPA filters for cab filtration of underground mining equipment.

PubMed

Cecala, A B; Organiscak, J A; Noll, J D; Zimmer, J A

2016-08-01

Significant strides have been made in optimizing the design of filtration and pressurization systems used on the enclosed cabs of mobile mining equipment to reduce respirable dust and provide the best air quality to the equipment operators. Considering all of the advances made in this area, one aspect that still needed to be evaluated was a comparison of the efficiencies of the different filters used in these systems. As high-efficiency particulate arrestance (HEPA) filters provide the highest filtering efficiency, the general assumption would be that they would also provide the greatest level of protection to workers. Researchers for the U.S. National Institute for Occupational Safety and Health (NIOSH) speculated, based upon a previous laboratory study, that filters with minimum efficiency reporting value, or MERV rating, of 16 may be a more appropriate choice than HEPA filters in most cases for the mining industry. A study was therefore performed comparing HEPA and MERV 16 filters on two kinds of underground limestone mining equipment, a roof bolter and a face drill, to evaluate this theory. Testing showed that, at the 95-percent confidence level, there was no statistical difference between the efficiencies of the two types of filters on the two kinds of mining equipment. As the MERV 16 filters were less restrictive, provided greater airflow and cab pressurization, cost less and required less-frequent replacement than the HEPA filters, the MERV 16 filters were concluded to be the optimal choice for both the roof bolter and the face drill in this comparative-analysis case study. Another key finding of this study is the substantial improvement in the effectiveness of filtration and pressurization systems when using a final filter design.

"A gift from heaven" or "This was not for me". A mixed methods approach to describe experiences of participation in an outsourced physical activity program for persons with rheumatoid arthritis.

PubMed

Demmelmaier, Ingrid; Lindkvist, Åsa; Nordgren, Birgitta; Opava, Christina H

2015-03-01

Most persons with rheumatoid arthritis (RA) do not perform health-enhancing physical activity (HEPA). Evaluations of innovative HEPA programs need to be complemented with descriptive and qualitative data from the users. The aim of this mixed methods study was to explore and describe how a subgroup of the RA population perceives participation during the first year of an outsourced 2-year HEPA program. Data were collected by a study-specific postal survey to 220 program participants (response rate 87%, n = 191) and by interviews with a purposefully selected subsample of 35, including completers and dropouts. The survey data were analyzed by descriptive statistics and the interviews by qualitative content analysis.The survey demonstrated increased self-reported physical activity in 165 participants (86%). After the first year of the program, participants reported having performed "regular" or "periodical" circuit training (78%) and physical activity in daily life (92%). The most valued program components were circuit training and physical activity in daily life, both rated median 5/5. Coach support, prompts by text messages, and expert lectures were rated median 4/5. Five categories emerged from the interviews describing expectations, facilitators, gains, maintenance, and obstacles/suggestions for improvement of the program. The results demonstrate that HEPA outside health care is highly appreciated by a subgroup of the RA population. Professional coaching and prompts by text messages seem to be particularly useful facilitators. Individual preferences emphasize the need to tailor settings, exercise formats, and behavioral support for HEPA even in a narrow, self-selected group with RA.

Comparison of MERV 16 and HEPA filters for cab filtration of underground mining equipment

PubMed Central

Cecala, A.B.; Organiscak, J.A.; Noll, J.D.; Zimmer, J.A.

2016-01-01

Significant strides have been made in optimizing the design of filtration and pressurization systems used on the enclosed cabs of mobile mining equipment to reduce respirable dust and provide the best air quality to the equipment operators. Considering all of the advances made in this area, one aspect that still needed to be evaluated was a comparison of the efficiencies of the different filters used in these systems. As high-efficiency particulate arrestance (HEPA) filters provide the highest filtering efficiency, the general assumption would be that they would also provide the greatest level of protection to workers. Researchers for the U.S. National Institute for Occupational Safety and Health (NIOSH) speculated, based upon a previous laboratory study, that filters with minimum efficiency reporting value, or MERV rating, of 16 may be a more appropriate choice than HEPA filters in most cases for the mining industry. A study was therefore performed comparing HEPA and MERV 16 filters on two kinds of underground limestone mining equipment, a roof bolter and a face drill, to evaluate this theory. Testing showed that, at the 95-percent confidence level, there was no statistical difference between the efficiencies of the two types of filters on the two kinds of mining equipment. As the MERV 16 filters were less restrictive, provided greater airflow and cab pressurization, cost less and required less-frequent replacement than the HEPA filters, the MERV 16 filters were concluded to be the optimal choice for both the roof bolter and the face drill in this comparative-analysis case study. Another key finding of this study is the substantial improvement in the effectiveness of filtration and pressurization systems when using a final filter design. PMID:27524838

Characteristics of insufficiently active participants that benefit from health-enhancing physical activity (HEPA) promotion programs implemented in the sports club setting.

PubMed

Ooms, Linda; Leemrijse, Chantal; Collard, Dorine; Schipper-van Veldhoven, Nicolette; Veenhof, Cindy

2018-06-01

Health-enhancing physical activity (HEPA) promotion programs are implemented in sports clubs. The purpose of this study was to examine the characteristics of the insufficiently active participants that benefit from these programs. Data of three sporting programs, developed for insufficiently active adults, were used for this study. These sporting programs were implemented in different sports clubs in the Netherlands. Participants completed an online questionnaire at baseline and after six months (n = 458). Of this sample, 35.1% (n = 161) was insufficiently active (i.e. not meeting HEPA levels) at baseline. Accordingly, two groups were compared: participants who were insufficiently active at baseline, but increased their physical activity to HEPA levels after six months (activated group, n = 86) versus participants who were insufficiently active both at baseline and after six months (non-activated group, n = 75). Potential associated characteristics (demographic, social, sport history, physical activity) were included as independent variables in bivariate and multivariate logistic regression analyses. The percentage of active participants increased significantly from baseline to six months (from 64.9 to 76.9%, p < 0.05). The bivariate logistic regression analyses showed that participants in the activated group were more likely to receive support from family members with regard to their sport participation (62.8% vs. 42.7%, p = 0.02) and spent more time in moderate-intensity physical activity (128 ± 191 min/week vs. 70 ± 106 min/week, p = 0.02) at baseline compared with participants in the non-activated group. These results were confirmed in the multivariate logistic regression analyses: when receiving support from most family members, there is a 216% increase in the odds of being in the activated group (OR = 2.155; 95% CI: 1.118-4.154, p = 0.02) and for each additional 1 min/week spent in moderate-intensity physical activity, the odds increases with 0.3% (OR = 1.003; 95% CI: 1.001-1.006, p = 0.02). The results suggest that HEPA sporting programs can be used to increase HEPA levels of insufficiently active people, but it seems a challenge to reach the least active ones. It is important that promotional strategies and channels are tailored to the target group. Furthermore, strategies that promote family support may enhance the impact of the programs.

Summary of efficiency testing of standard and high-capacity high-efficiency particulate air filters subjected to simulated tornado depressurization and explosive shock waves

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, P.R.; Gregory, W.S.

1985-04-01

Pressure transients in nuclear facility air cleaning systems can originate from natural phenomena such as tornadoes or from accident-induced explosive blast waves. This study was concerned with the effective efficiency of high-efficiency particulate air (HEPA) filters during pressure surges resulting from simulated tornado and explosion transients. The primary objective of the study was to examine filter efficiencies at pressure levels below the point of structural failure. Both standard and high-capacity 0.61-m by 0.61-m HEPA filters were evaluated, as were several 0.2-m by 0.2-m HEPA filters. For a particular manufacturer, the material release when subjected to tornado transients is the samemore » (per unit area) for both the 0.2-m by 0.2-m and the 0.61-m by 0.61-m filters. For tornado transients, the material release was on the order of micrograms per square meter. When subjecting clean HEPA filters to simulated tornado transients with aerosol entrained in the pressure pulse, all filters tested showed a degradation of filter efficiency. For explosive transients, the material release from preloaded high-capacity filters was as much as 340 g. When preloaded high-capacity filters were subjected to shock waves approximately 50% of the structural limit level, 1 to 2 mg of particulate was released.« less

Growth-inhibitory effects of the red alga Gelidium amansii on cultured cells.

PubMed

Chen, Yue-Hwa; Tu, Ching-Jung; Wu, Hsiao-Ting

2004-02-01

The objective of this study was to investigate the effects of Gelidium amansii, an edible red agar cultivated off the northeast coast of Taiwan, on the growth of two lines of cancer cells, murine hepatoma (Hepa-1) and human leukemia (HL-60) cells, as well as a normal cell line, murine embryo fibroblast cells (NIH-3T3). The potential role of G. amansii on the induction of apoptosis was also examined. The results indicated that all extracts from G. amansii, including phosphate-buffered saline (PBS) and methanol extracts from dried algae as well as the dimethyl sulfoxide (DMSO) extract from freeze-dried G. amansii agar, inhibited the growth of Hepa-1 and NIH-3T3 cells, but not the growth of HL-60 cells. Annexin V-positive cells were observed in methanol and DMSO extract-treated, but not PBS extract-treated Hepa-1 and NIH-3T3 cells, suggesting that the lipid-soluble extracts of G. amansii induced apoptosis. In summary, extracts of G. amansii from various preparations exhibited antiproliferative effects on Hepa-1 and NIH-3T3 cells, and apoptosis may play a role in the methanol and DMSO extract-induced inhibitory effects. However, the antiproliferative effects of PBS extracts was not through apoptosis. Moreover, the growth-inhibitory effects of G. amansii were not specific to cancer cells.

A Feasibility Study for the Laboratory Reduction of Low Level Radioactive Wastes

DTIC Science & Technology

1988-04-01

the Los Alamos National Laboratory), pass the off-gases through a quench column, venturi scrubber , packed column. cordenser, de-mi ste-, re-heater...treatment of the off-gas. In addition to HEPA filters, all of the fully developed systems used sore type of scrubber in the exhaust system to remove...considerations shown in the table and the desired use of a muffle furnace with just a HEPA filter for the off-gas (no scrubbers ), only the slow burn ashing

Influence of argon and oxygen on charge-state-resolved ion energy distributions of filtered aluminum arcs

NASA Astrophysics Data System (ADS)

Rosén, Johanna; Anders, André; Mráz, Stanislav; Atiser, Adil; Schneider, Jochen M.

2006-06-01

The charge-state-resolved ion energy distributions (IEDs) in filtered aluminum vacuum arc plasmas were measured and analyzed at different oxygen and argon pressures in the range of 0.5-8.0 mTorr. A significant reduction of the ion energy was detected as the pressure was increased, most pronounced in an argon environment and for the higher charge states. The corresponding average charge state decreased from 1.87 to 1.0 with increasing pressure. The IEDs of all metal ions in oxygen were fitted with shifted Maxwellian distributions. The results show that it is possible to obtain a plasma composition with a narrow charge-state distribution as well as a narrow IED. These data may enable tailoring thin film properties through selecting growth conditions that are characterized by predefined charge state and energy distributions.

ASME AG-1 Section FC Qualified HEPA Filters; a Particle Loading Comparison - 13435

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stillo, Andrew; Ricketts, Craig I.

High Efficiency Particulate Air (HEPA) Filters used to protect personnel, the public and the environment from airborne radioactive materials are designed, manufactured and qualified in accordance with ASME AG-1 Code section FC (HEPA Filters) [1]. The qualification process requires that filters manufactured in accordance with this ASME AG-1 code section must meet several performance requirements. These requirements include performance specifications for resistance to airflow, aerosol penetration, resistance to rough handling, resistance to pressure (includes high humidity and water droplet exposure), resistance to heated air, spot flame resistance and a visual/dimensional inspection. None of these requirements evaluate the particle loading capacitymore » of a HEPA filter design. Concerns, over the particle loading capacity, of the different designs included within the ASME AG-1 section FC code[1], have been voiced in the recent past. Additionally, the ability of a filter to maintain its integrity, if subjected to severe operating conditions such as elevated relative humidity, fog conditions or elevated temperature, after loading in use over long service intervals is also a major concern. Although currently qualified HEPA filter media are likely to have similar loading characteristics when evaluated independently, filter pleat geometry can have a significant impact on the in-situ particle loading capacity of filter packs. Aerosol particle characteristics, such as size and composition, may also have a significant impact on filter loading capacity. Test results comparing filter loading capacities for three different aerosol particles and three different filter pack configurations are reviewed. The information presented represents an empirical performance comparison among the filter designs tested. The results may serve as a basis for further discussion toward the possible development of a particle loading test to be included in the qualification requirements of ASME AG-1 Code sections FC and FK[1]. (authors)« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sievers, David A.; Kuhn, Erik M.; Tucker, Melvin P.

In this study, the reaction conditions used during dilute-acid pretreatment of lignocellulosic biomass control the carbohydrate digestion yield and also hydrolyzate properties. Depending on the conversion route of interest, solid-liquid separation (SLS) may be required to split the hemicellulose-rich liquor from the cellulose-rich insoluble solids, and slurry properties are important for SLS. Corn stover was pretreated at different reaction conditions and the slurries were assessed for conversion yield and filtration performance. Increasing pretreatment temperature reduced the solids mean particle size and resulted in slower slurry filtration rates when vacuum filtered or pressure filtered. Corn stover pretreated at 165 °C formore » 10 min and with 1% H 2SO 4 exhibited the highest xylose yield and best filtration performance with a no-wash filtration rate of 80 kg/h m 2 and cake permeability of 15 x 10 -15.« less

Fungal spore concentrations in two haematopoietic stem cell transplantation (HSCT) units containing distinct air control systems.

PubMed

Brun, C P; Miron, D; Silla, L M R; Pasqualotto, A C

2013-04-01

Invasive fungal diseases have emerged as important causes of morbidity and mortality in haematological patients. In this study air samples were collected in two haematopoietic stem cell transplantation (HSCT) units, in which distinct air-control systems were in place. In hospital 1 no high-efficiency particulate air (HEPA) filter was available whereas in hospital 2 HSCT rooms were equipped with HEPA filters, with positive air pressure in relation to the corridor. A total of 117 samples from rooms, toilets and corridors were obtained during December 2009 to January 2011, using a six-stage Andersen sampler. In both hospitals, the concentration of potentially pathogenic fungi in the air was reduced in patients' rooms compared to corridors (P < 0·0001). Despite the presence of a HEPA filter in hospital 2, rooms in both hospitals showed similar concentrations of potentially pathogenic fungi (P = 0·714). These findings may be explained by the implementation of additional protective measures in hospital 1, emphasizing the importance of such measures in protected environments.

Effects of dilute-acid pretreatment conditions on filtration performance of corn stover hydrolyzate

DOE PAGES

Sievers, David A.; Kuhn, Erik M.; Tucker, Melvin P.; ...

2017-06-28

In this study, the reaction conditions used during dilute-acid pretreatment of lignocellulosic biomass control the carbohydrate digestion yield and also hydrolyzate properties. Depending on the conversion route of interest, solid-liquid separation (SLS) may be required to split the hemicellulose-rich liquor from the cellulose-rich insoluble solids, and slurry properties are important for SLS. Corn stover was pretreated at different reaction conditions and the slurries were assessed for conversion yield and filtration performance. Increasing pretreatment temperature reduced the solids mean particle size and resulted in slower slurry filtration rates when vacuum filtered or pressure filtered. Corn stover pretreated at 165 °C formore » 10 min and with 1% H 2SO 4 exhibited the highest xylose yield and best filtration performance with a no-wash filtration rate of 80 kg/h m 2 and cake permeability of 15 x 10 -15.« less

Effects of dilute-acid pretreatment conditions on filtration performance of corn stover hydrolyzate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sievers, David A.; Kuhn, Erik M.; Tucker, Melvin P.

In this study, the reaction conditions used during dilute-acid pretreatment of lignocellulosic biomass control the carbohydrate digestion yield and also hydrolyzate properties. Depending on the conversion route of interest, solid-liquid separation (SLS) may be required to split the hemicellulose-rich liquor from the cellulose-rich insoluble solids, and slurry properties are important for SLS. Corn stover was pretreated at different reaction conditions and the slurries were assessed for conversion yield and filtration performance. Increasing pretreatment temperature reduced the solids mean particle size and resulted in slower slurry filtration rates when vacuum filtered or pressure filtered. Corn stover pretreated at 165 °C formore » 10 min and with 1% H 2SO 4 exhibited the highest xylose yield and best filtration performance with a no-wash filtration rate of 80 kg/h m 2 and cake permeability of 15 x 10 -15.« less

Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice.

PubMed

Araújo, Tiago Gomes; Oliveira, Alexandre Gabarra; Vecina, Juliana Falcato; Marin, Rodrigo Miguel; Franco, Eryvelton Souza; Abdalla Saad, Mario J; de Sousa Maia, Maria Bernadete

2016-08-01

Parkinsonia aculeata L. (Caesalpiniaceae) is a traditional ethnomedicine and has been used for the empiric treatment of hyperglycemia, without scientific background. Mechanistic analyses at molecular level from the antioxidant mechanism observed by P. aculeata are required. Herein the effects of the treatment by hydroethanolic extract partitioned with ethyl acetate of P. aculeata aerial parts (HEPa/EtOAc) in mice fed a high-fat diet that share many obesity phenotypes with humans were evaluated. The animals were treated orally with HEPa/EtOAc (125 and 250 mg/kg/day) and pioglitazone (5 mg/kg/day), for 16 days. After the treatment, HEPa/EtOAc reduced fasting serum glucose and insulin levels, as well as homeostasis model assessment for insulin resistance. In addition, an improvement in glucose intolerance was also observed. Indeed, a reduction in the circulating levels of TNF-α and IL-6 was also observed. Furthermore, at molecular level, it was demonstrated that the HEPa/EtOAc treatment was able to improve these physiological parameters, through the activation of peroxisome proliferator-activated receptor γ (PPARγ) per si, as well as the enhancement of antioxidant mechanism by an increase in PPARγ/Cu(2+), Zn(2+)-superoxide dismutase (CuZn-SOD) axis expression in liver and adipose tissue. In sum, P. aculeata is effective to improve insulin resistance in a mouse model of obesity and this effect seems to involve the antioxidant and anti-inflammatory mechanisms through the increase in PPARγ/CuZn-SOD axis expression.

The Start2Bike program is effective in increasing health-enhancing physical activity: a controlled study.

PubMed

Ooms, Linda; Veenhof, Cindy; de Bakker, Dinny H

2017-06-29

The sports club is seen as a new relevant setting to promote health-enhancing physical activity (HEPA) among inactive population groups. Little is known about the effectiveness of strategies and activities implemented in the sports club setting on increasing HEPA levels. This study investigated the effects of Start2Bike, a six-week training program for inactive adults and adult novice cyclers, on HEPA levels of participants in the Netherlands. To measure physical activity, the Short QUestionnaire to ASsess Health-enhancing physical activity was used (SQUASH). Start2Bike participants were measured at baseline, six weeks and six months. A matched control group was measured at baseline and six months. The main outcome measure was whether participants met the Dutch Norm for Health-enhancing Physical Activity (DNHPA: 30 min of moderate-intensity activity on five days a week); Fit-norm (20 min of vigorous-intensity activity on three days a week); and Combi-norm (meeting the DNHPA and/or Fit-norm). Other outcome measures included: total minutes of physical activity per week; and minutes of physical activity per week per domain and intensity category. Statistical analyses consisted of McNemar tests and paired t-tests (within-group changes); and multiple logistic and linear regression analyses (between-group changes). In the Start2Bike group, compliance with Dutch physical activity norms increased significantly, both after six weeks and six months. Control group members did not alter their physical activity behavior. Between-group analyses showed that participants in the Start2Bike group were more likely to meet the Fit-norm at the six-month measurement compared to the control group (odds ratio = 2.5; 95% confidence interval (CI) = 1.1-5.8, p = 0.03). This was due to the Start2Bike participants spending on average 193 min/week more in vigorous-intensity activities (b = 193; 95% CI = 94-293, p < 0.001) and 130 min/week more in sports activities (b = 130; 95% CI = 82-178, p < 0.001) than control group members. Start2Bike positively influences HEPA levels of participants by increasing participation in sport. A relatively short sporting program, offered by a sports club, can be used to encourage less active people to engage in and continue sport at HEPA levels. Overall, sport can contribute to health through increased HEPA and the sports club can serve as a setting to stimulate this.

Isolation, Cloning and Expression of the Genes for Microbial Polyurethane Degradation

DTIC Science & Technology

1991-05-31

utilizing lysing enzymes, Gluculase and Novozyme , digest the mycelium and produce protoplasts but also tend to digest the DNA. The detergent procedures did...at -20 ’C. Novozyme Procedure One liter of fresh HAFB-2F-Br culture, grown to saturation, was vacuum filtered through Whatman filter paper, #40...resuspended in buffer (15% sucrose, 50 mM Tris pH 7.6, 50 mM EDTA) at 0.2 g filtrate/ml buffer in 15 ml centrifuge tubes. Novozyme was added at 40 mg/ml and

[Comparison of antibody persistence between live attenuated and inactivated hepatitis A vaccines].

PubMed

Liu, Huai-Feng; Zhang, Xin-Jiang; Zhang, Jian-Li

2009-08-01

To study the antibody persistence of live attenuated hepatitits A vaccine, and to compare the antibody between with inactivated vaccine. 211 HAV susceptible children were divided randomly into three groups, Group A was injected three doses HepA-L at 0, 6 and 12 monthes; Group B was administrated two dose HepA-L at 0 and 6 months, and group C was immunized with inactivated vaccine at month 0 and 6. Serum samples were detected for Anti-HAV at 1, 6, 7, 12, 13, 24, 84 months after vaccination in each group. The seroconversion rates reached 100% after 2nd dose in all groups. The highest GMC was 2938.1 mlU/ml, founded in group C, and it was 1315.6 mlU/ml and 1586 mlU/ml in group A and B respectively. After the 3rd dose at month 12 in group A, the antibody increased dramatic, which reached 1945.3 mlU/ml. 84 months after first dose in each group, the antibody can be detected from all subjects. Though the GMC in group A declined to 336.8 mlU/ml, it was significant higher than that in group B and C. The good booster effect with HepA-L was well observed in a short-term. The immune response induced by 2 to 3 doses HepA-L could compete with inactivated hepatitis A vaccine. However, long-term effects of both vaccines need further study.

Electrostatic dust detector

DOEpatents

Skinner, Charles H [Lawrenceville, NJ

2006-05-02

An apparatus for detecting dust in a variety of environments which can include radioactive and other hostile environments both in a vacuum and in a pressurized system. The apparatus consists of a grid coupled to a selected bias voltage. The signal generated when dust impacts and shorts out the grid is electrically filtered, and then analyzed by a signal analyzer which is then sent to a counter. For fine grids a correlation can be developed to relate the number of counts observed to the amount of dust which impacts the grid.

Xenobiotic metabolism in the fish hepatic cell lines Hepa-E1 and RTH-149, and the gill cell lines RTgill-W1 and G1B: Biomarkers of CYP450 activity and oxidative stress.

PubMed

Franco, Marco E; Sutherland, Grace E; Lavado, Ramon

2018-04-01

The use of fish cell cultures has proven to be an effective tool in the study of environmental and aquatic toxicology. Valuable information can be obtained from comparisons between cell lines from different species and organs. In the present study, specific chemicals were used and biomarkers (e.g. 7-Ethoxyresorufin-O-deethylase (EROD) activity and reactive oxygen species (ROS)) were measured to assess the metabolic capabilities and cytotoxicity of the fish hepatic cell lines Hepa-E1 and RTH-149, and the fish gill cell lines RTgill-W1 and G1B. These cell lines were exposed to β-naphthoflavone (BNF) and benzo[a]pyrene (BaP), the pharmaceutical tamoxifen (TMX), and the organic peroxide tert-butylhydroperoxide (tBHP). Cytotoxicity in gill cell lines was significantly higher than in hepatic cells, with BNF and TMX being the most toxic compounds. CYP1-like associated activity, measured through EROD activity, was only detected in hepatic cells; Hepa-E1 cells showed the highest activity after exposure to both BNF and BaP. Significantly higher levels of CYP3A-like activity were also observed in Hepa-E1 cells exposed to TMX, while gill cell lines presented the lowest levels. Measurements of ROS and antioxidant enzymes indicated that peroxide levels were higher in gill cell lines in general. However, levels of superoxide were significantly higher in RTH-149 cells, where no distinctive increase of superoxide-related antioxidants was observed. The present study demonstrates the importance of selecting adequate cell lines in measuring specific metabolic parameters and provides strong evidence for the fish hepatocarcinoma Hepa-E1 cells to be an excellent alternative in assessing metabolism of xenobiotics, and in expanding the applicability of fish cell lines for in vitro studies. Copyright © 2018 Elsevier Inc. All rights reserved.

Platelet adhesion on phosphorus-incorporated tetrahedral amorphous carbon films

NASA Astrophysics Data System (ADS)

Liu, Aiping; Zhu, Jiaqi; Liu, Meng; Dai, Zhifei; Han, Xiao; Han, Jiecai

2008-11-01

The haemocompatibility of phosphorus-incorporated tetrahedral amorphous carbon (ta-C:P) films, synthesized by filtered cathodic vacuum arc technique with PH 3 as the dopant source, was assessed by in vitro platelet adhesion tests. Results based on scanning electron microscopy and contact angle measurements reveal that phosphorus incorporation improves the wettability and blood compatibility of ta-C film. Our studies may provide a novel approach for the design and synthesis of doped ta-C films to repel platelet adhesion and reduce thrombosis risk.

Fundamental Studies on Reactive Oligomers

DTIC Science & Technology

1974-04-01

filtered off, washed well with water and vacuum-dried at 50 0 C to afford 7.031 g of unreacted 4-chlorophenyl sulfone, identified by its melting point ...hours. The melting point was l00-130*C; the infrared spectrum was virtually identical with that of 4-chlorophenyl sulfone. 13. Preparation of Bis[4-(3...excess of 550°C. On a Fisher-Johns melting point apparatus BTPN-l melted over the range 255-285°C and did not harden in 10 minutes at 3000 C. BTPN-l was

Changes in hepatitis A and B vaccination rates in adult patients with chronic liver diseases and diabetes in the U.S. population.

PubMed

Younossi, Zobair M; Stepanova, Maria

2011-10-01

Professional societies recommend hepatitis A and hepatitis B immunization for individuals with chronic liver disease (CLD), but the degree of implementation is unknown. Data were obtained from the National Health and Nutrition Examination Surveys (NHANES) conducted in 1999-2008. For the entire study population and for those with CLD and diabetes, we determined the rates and independent predictors of history of hepatitis A and hepatitis B (HepA and HepB) vaccinations, of their effectiveness, and of seroprevalence of hepatitis A antibody and anti-HB surface antibody. In total, 24,871 participants from NHANES were included: 14,886 (1999-2004) and 9,985 (2005-2008). Of these individuals, 14.0% had CLD and 8.6% had diabetes. During the study period, HepA vaccination in CLD increased from 13.3% ± 1.0% to 20.0% ± 1.5%, HepB vaccination increased from 23.4% ± 1.2% to 32.1% ± 1.5%. Of subtypes of CLD, HepA vaccination rates increased only in nonalcoholic fatty liver disease (NAFLD), whereas HepB vaccination increased for patients with hepatitis C and nonalcoholic fatty liver disease. In the diabetic cohort, HepA vaccination rates increased from 9.3% ± 1.1% to 15.4% ± 1.7% and HepB rates increased from 15.2% ± 1.5% to 22.4% ± 1.7%. All changes were similar to those observed in the general population. The quality measure (QM) for HepA in the general population decreased from 44.4% ± 1.2% in 1999-2004 to 41.7% ± 1.9% in 2005-2008, and similar changes were noted for all subcohorts. On the other hand, QM for HepB increased from 31.7% ± 0.9% to 40.7% ± 1.0% in the population, whereas no changes in QM were noted in any diagnostic cohort except for NAFLD. Although vaccination rates in CLD and diabetic cohorts are increasing, they remain low. Given the public health implications of acute hepatitis A and hepatitis B in patients with CLD, better implementation of the vaccination recommendations for these populations is warranted. Copyright © 2011 American Association for the Study of Liver Diseases.

Fire tests to evaluate the potential fire threat and its effects on HEPA filter integrity in cell ventilation at the Oak Ridge National Laboratory, Building 7920

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hasegawa, H.K.; Staggs, K.J.; Doughty, S.M.

1992-12-01

As a result of a DOE (Tiger Team) Technical Safety Appraisal (November 1990) of the Radiochemical Engineering Development Center (REDC), ORNL Building 7920, a number of fire protection concerns were identified. The primary concern was the perceived loss of ventilation system containment due to the thermal destruction and/or breaching of the prefilters and/or high-efficiency particulate air filters (HEPA `s) and the resultant radioactive release to the external environment. The following report describes the results of an extensive fire test program performed by the Fire Research Discipline (FRD) of the Special Projects Division of Lawrence Livermore National Lab (LLNL) and fundedmore » by ORNL to address these concerns. Full scale mock-ups of a REDC hot cell tank pit, adjacent cubicle pit, and associated ventilation system were constructed at LLNL and 13 fire experiments were conducted to specifically answer the questions raised by the Tiger Team. Our primary test plan was to characterize the burning of a catastrophic solvent spill (kerosene) of 40 liters and its effect on the containment ventilation system prefilters and HEPA filters. In conjunction with ORNL and Lockwood Greene we developed a test matrix that assessed the fire performance of the prefilters and HEPA filters; evaluated the fire response of the fiber reinforced plastic (FRP) epoxy ventilation duct work; the response and effectiveness of the fire protection system, the effect of fire in a cubicle on the vessel off-gas (VOG) elbow, and other fire safety questions.« less

Self-reported hepatitis A vaccination as a predictor of hepatitis A virus antibody protection in U.S. adults: National Health and Nutrition Examination Survey 2007-2012.

PubMed

Denniston, Maxine M; Monina Klevens, R; Jiles, Ruth B; Murphy, Trudy V

2015-07-31

To estimate the predictive value of self-reported hepatitis A vaccine (HepA) receipt for the presence of hepatitis A virus (HAV) antibody (anti-HAV) from either past infection or vaccination, as an indicator of HAV protection. Using 2007-2012 National Health and Nutrition Examination Survey data, we assigned participants to 4 groups based on self-reported HepA receipt and anti-HAV results. We compared characteristics across groups and calculated three measures of agreement between self-report and serologic status (anti-HAV): percentage concordance, and positive (PPV) and negative (NPV) predictive values. Using logistic regression we investigated factors associated with agreement between self-reported vaccination status and serological results. Demographic and other characteristics varied significantly across the 4 groups. Overall agreement between self-reported HepA receipt and serological results was 63.6% (95% confidence interval [CI] 61.9-65.2); PPV and NPV of self-reported vaccination status for serological result were 47.0% (95% CI 44.2-49.8) and 69.4% (95% CI 67.0-71.8), respectively. Mexican American and foreign-born adults had the highest PPVs (71.5% [95% CI 65.9-76.5], and 75.8% [95% CI 71.4-79.7]) and the lowest NPVs (21.8% [95% CI 18.5-25.4], and 20.0% [95% CI 17.2-23.1]), respectively. Young (ages 20-29 years), US-born, and non-Hispanic White adults had the lowest PPVs (37.9% [95% CI 34.5-41.5], 39.1% [95% CI, 36.0-42.3], and 39.8% [36.1-43.7]), and the highest NPVs (76.9% [95% CI 72.2-81.0, 78.5% [95% CI 76.5-80.4)], and 80.6% [95% CI 78.2-82.8), respectively. Multivariate logistic analyses found age, race/ethnicity, education, place of birth and income to be significantly associated with agreement between self-reported vaccination status and serological results. When assessing hepatitis A protection, self-report of not having received HepA was most likely to identify persons at risk for hepatitis A infection (no anti-HAV) among young, US-born and non-Hispanic White adults, and self-report of HepA receipt was least likely to be reliable among adults with the same characteristics. Copyright © 2015. Published by Elsevier Ltd.

HEPA Filter Disposal Write-Up 10/19/16

DOE Office of Scientific and Technical Information (OSTI.GOV)

Loll, C.

Process knowledge (PK) collection on HEPA filters is handled via the same process as other waste streams at LLNL. The Field technician or Characterization point of contact creates an information gathering document (IGD) in the IGD database, with input provided from the generator, and submits it for electronic approval. This document is essentially a waste generation profile, detailing the physical, chemical as well as radiological characteristics, and hazards, of a waste stream. It will typically contain a general, but sometimes detailed, description of the work processes which generated the waste. It will contain PK as well as radiological and industrialmore » hygiene analytical swipe results, and any other analytical or other supporting knowledge related to characterization. The IGD goes through an electronic approval process to formalize the characterization and to ensure the waste has an appropriate disposal path. The waste generator is responsible for providing initial process knowledge information, and approves the IGD before it routed to chemical and radiological waste characterization professionals. This is the standard characterization process for LLNL-generated HEPA Filters.« less

Self-Assembled Lipid Nanoparticles for Oral Delivery of Heparin-Coated Iron Oxide Nanoparticles for Theranostic Purposes.

PubMed

Truzzi, Eleonora; Bongio, Chiara; Sacchetti, Francesca; Maretti, Eleonora; Montanari, Monica; Iannuccelli, Valentina; Vismara, Elena; Leo, Eliana

2017-06-09

Recently, solid lipid nanoparticles (SLNs) have attracted increasing attention owing to their potential as an oral delivery system, promoting intestinal absorption in the lymphatic circulation which plays a role in disseminating metastatic cancer cells and infectious agents throughout the body. SLN features can be exploited for the oral delivery of theranostics. Therefore, the aim of this work was to design and characterise self-assembled lipid nanoparticles (SALNs) to encapsulate and stabilise iron oxide nanoparticles non-covalently coated with heparin (Fe@hepa) as a model of a theranostic tool. SALNs were characterised for physico-chemical properties (particle size, surface charge, encapsulation efficiency, in vitro stability, and heparin leakage), as well as in vitro cytotoxicity by methyl thiazole tetrazolium (MTT) assay and cell internalisation in CaCo-2, a cell line model used as an indirect indication of intestinal lymphatic absorption. SALNs of about 180 nm, which are stable in suspension and have a high encapsulation efficiency (>90%) were obtained. SALNs were able to stabilise the heparin coating of Fe@hepa, which are typically unstable in physiological environments. Moreover, SALNs-Fe@hepa showed no cytotoxicity, although their ability to be internalised into CaCo-2 cells was highlighted by confocal microscopy analysis. Therefore, the results indicated that SALNs can be considered as a promising tool to orally deliver theranostic Fe@hepa into the lymphatic circulation, although further in vivo studies are needed to comprehend further potential applications.

Invasive fungal infection following chemotherapy for acute myeloid leukaemia-Experience from a developing country.

PubMed

Korula, Anu; Abraham, Aby; Abubacker, Fouzia N; Viswabandya, Auro; Lakshmi, Kavitha M; Abraham, O C; Rupali, Priscilla; Varghese, George M; Michael, Joy S; Srivastava, Alok; Mathews, Vikram; George, Biju

2017-10-01

The incidence of invasive fungal infections (IFI) is believed to be higher in patients with acute myeloid leukaemia (AML) undergoing chemotherapy in non-HEPA-filtered rooms. The aim of this study is to review the incidence of IFI in a large cohort of patients with AML treated at a single centre in India. Two hundred and twenty-two patients with AML treated with either induction chemotherapy or salvage chemotherapy between 2008 and 2013 were studied retrospectively. IFI was defined as per the revised EORTC-MSG criteria. Data on type of chemotherapy, prophylactic strategies, engraftment (ANC>500), the presence of IFI and survival were collected. IFI was diagnosed in 86 patients (38.7%) with proven IFI in 12 (5.4%). Use of posaconazole prophylaxis (P=.001) was the only factor associated with reduced incidence of IFI. Survival in patients with proven IFI was lower than those without proven IFI, but not statistically significant (59.4% vs 78.5%; P=.139). There is a high incidence of IFI during induction chemotherapy for acute myeloid leukaemia in developing countries. Posaconazole prophylaxis was associated with a significantly lower incidence of IFI. Optimal yet cost-effective strategies for prevention and early diagnosis of IFI are required to improve survival in patients undergoing chemotherapy for AML. © 2017 Blackwell Verlag GmbH.

A pilot study to investigate the effects of combined dehumidification and HEPA filtration on dew point and airborne mold spore counts in day care centers.

PubMed

Bernstein, J A; Levin, L; Crandall, M S; Perez, A; Lanphear, B

2005-12-01

Meteorological factors such as relative humidity directly correlate with airborne fungal levels outdoors and indoors. While dehumidification alone is effective at reducing moisture necessary for mold growth, it is inadequate as a single intervention as it does not remove viable and non-viable fungal spores that are potentially allergenic. The purpose of this pilot study was to investigate whether dehumidification in combination with high-efficiency particulate arrestance (HEPA) filtration is effective at reducing airborne mold spore levels in day care centers. Two day care centers within a 2-mile radius of each other were selected. Day care center A was 2 years old with eight rooms while day care center B was 15 years old with six rooms. A high efficiency Santa Fe dehumidification unit equipped with a HEPA filter was installed in half the rooms (intervention) of each day care facility. Electronic HOBO data loggers continuously measured outdoor and indoor room dew point and temperature every 2 h throughout the study. Dew point and airborne fungal spore measurements from selected rooms with controlled air conditions were analyzed by comparing baseline measurements to those obtained at subsequent time periods over 1 year. Regression models accounted for correlations between measurements in the same room over time. Intervention resulted in a lowered average dew point from baseline by 8.8 degrees C compared with a decrease of 7.1 degrees C in non-intervention rooms across all time periods in both facilities (P<0.001). Fungal analyses demonstrated lower baseline (P=0.06) and follow-up means in intervention rooms (P<0.05), however the change from baseline to end of follow-up differed between intervention and non-intervention rooms in the two facilities. Log transformation was applied to approximate normality of fungal measurements. Dehumidification with HEPA filtration was effective at controlling indoor dew point in both facilities and at reducing airborne culturable fungal spore levels in one of the two facilities. These preliminary results provide a scientific rationale for using this intervention in future studies designed to investigate the impact of indoor mold exposure on health outcomes. Poor indoor air quality is a recognized cause or contributing factor to health effects. Dampness and humidity have been linked to upper and lower respiratory symptoms in children and adults. This study indicates that reducing indoor relative humidity and airborne mold spore levels using high-efficiency dehumidification units equipped with HEPA filtration is feasible even in work facilities such as day care centers where traffic in and out of the building is difficult to regulate. Clinicians should emphasize to their patients the importance of dehumidification and HEPA filtration to improve indoor air quality in the home and workplace.

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 3. Aerobiology.

PubMed

Bohannon, J Kyle; Janosko, Krisztina; Holbrook, Michael R; Barr, Jason; Pusl, Daniela; Bollinger, Laura; Coe, Linda; Hensley, Lisa E; Jahrling, Peter B; Wada, Jiro; Kuhn, Jens H; Lackemeyer, Matthew G

2016-10-03

Aerosol or inhalational studies of high-consequence pathogens have recently been increasing in number due to the perceived threat of intentional aerosol releases or unexpected natural aerosol transmission. Specific laboratories designed to perform these experiments require tremendous engineering controls to provide a safe and secure working environment and constant systems maintenance to sustain functionality. Class III biosafety cabinets, also referred to as gloveboxes, are gas-tight enclosures with non-opening windows. These cabinets are maintained under negative pressure by double high-efficiency-particulate-air (HEPA)-filtered exhaust systems and are the ideal primary containment for housing aerosolization equipment. A well planned workflow between staff members within high containment from, for instance, an animal biosafety level-4 (ABSL-4) suit laboratory to the ABSL-4 cabinet laboratory is a crucial component for successful experimentation. For smooth study execution, establishing a communication network, moving equipment and subjects, and setting up and placing equipment, requires staff members to meticulously plan procedures prior to study initiation. Here, we provide an overview and a visual representation of how aerobiology research is conducted at the National Institutes of Health, National Institute of Allergy and Infectious Diseases Integrated Research Facility at Fort Detrick, Maryland, USA, within an ABSL-4 environment.

Impact of humidification and nebulization during expiratory limb protection: an experimental bench study.

PubMed

Tonnelier, Alexandre; Lellouche, François; Bouchard, Pierre Alexandre; L'Her, Erwan

2013-08-01

Different filtering devices are used during mechanical ventilation to avoid dysfunction of flow and pressure transducers or for airborne microorganisms containment. Water condensates, resulting from the use of humidifiers, but also residual nebulization particles may have a major influence on expiratory limb resistance. To evaluate the influence of nebulization and active humidification on the resistance of expiratory filters. A respiratory system analog was constructed using a test lung, an ICU ventilator, heated humidifiers, and a piezoelectric nebulizer. Humidifiers were connected to different types of circuits (unheated, mono-heated, new-generation and old-generation bi-heated). Five filter types were evaluated: electrostatic, heat-and-moisture exchanger, standard, specific, and internal heated high-efficiency particulate air [HEPA] filter. Baseline characteristics were obtained from each dry filter. Differential pressure measurements were carried out after 24 hours of continuous in vitro use for each condition, and after 24 hours of use with an old-generation bi-heated circuit without nebulization. While using unheated circuits, measurements had to be interrupted before 24 hours for all the filtering devices except the internal heated HEPA filter. The heat-and-moisture exchangers occluded before 24 hours with the unheated and mono-heated circuits. The circuit type, nebulization practice, and duration of use did not influence the internal heated HEPA filter resistance. Expiratory limb filtration is likely to induce several major adverse events. Expiratory filter resistance increase is due mainly to the humidification circuit type, rather than to nebulization. If filtration is mandatory while using an unheated circuit, a dedicated filter should be used for ≤ 24 hours, or a heated HEPA for a longer duration.

Physical activity in adults with congenital heart disease and associations with functional outcomes.

PubMed

Müller, Jan; Amberger, Tamara; Berg, Anika; Goeder, Daniel; Remmele, Julia; Oberhoffer, Renate; Ewert, Peter; Hager, Alfred

2017-07-01

Improved survival has yielded to growing importance of functional outcome measures in patients with congenital heart disease (CHD). This study applied the International Physical Activity Questionnaire (IPAQ) to assess self-reported physical activity (PA) in patients with CHD and their association with exercise capacity and health-related quality of life (HrQoL). Prior to cardiopulmonary exercise testing (CPET), 786 consecutive patients (335 female, 31.1±11.6 years) with CHD filled in the short form of the IPAQ and the HrQoL questionnaire 36-Item Short Form. In total, 393 (50.0%) patients reported health-enhancing physical activity (HEPA), 237 (30.2%) minimal activity and 156 (19.8%) inactivity. In comparison with the HEPA group, the inactive group had significant lower peak oxygen uptake (74.2%±20.7% vs 86.8%±22.3%; p<0.001) as well as lower physical (91.0%±16.9% vs 97.4%±13.6%; p<0.001) and mental (97.1%±22.2% vs 104.1%±15.6%; p<0.001) HrQoL. Independent of severity class, surgery, age, beta-blocker, pacemaker and oxygen saturation, the HEPA group in comparison with the inactive group showed significantly less probability for impairments in mental (OR: 0.21, 95% CI: 0.10 to 0.45; p<0.001) and physical (OR: 0.46, 95% CI: 0.23 to 0.92; p=0.027) HrQoL and exercise capacity (OR: 0.36, 95% CI: 0.22 to 0.59; p<0.001). Categorisation of patientswith CHD with the IPAQ quickly provides clinical information as HEPA patients have a less probability for impaired HrQoL and diminished exercise capacity. Nevertheless, the IPAQ cannot substitute an accelerometer-based assessment for PA, nor a CPET for exercise capacity. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Cost-effectiveness analysis of catch-up hepatitis A vaccination among unvaccinated/partially-vaccinated children.

PubMed

Hankin-Wei, Abigail; Rein, David B; Hernandez-Romieu, Alfonso; Kennedy, Mallory J; Bulkow, Lisa; Rosenberg, Eli; Trigg, Monica; Nelson, Noele P

2016-07-29

Since 2006, the US Centers for Disease Control and Prevention has recommended hepatitis A (HepA) vaccination routinely for children aged 12-23months to prevent hepatitis A virus (HAV) infection. However, a substantial proportion of US children are unvaccinated and susceptible to infection. We present results of economic modeling to assess whether a one-time catch-up HepA vaccination recommendation would be cost-effective. We developed a Markov model of HAV infection that followed a single cohort from birth through death (birth to age 95years). The model compared the health and economic outcomes from catch-up vaccination interventions for children at target ages from two through 17years vs. outcomes resulting from maintaining the current recommendation of routine vaccination at age one year with no catch-up intervention. Over the lifetime of the cohort, catch-up vaccination would reduce the total number of infections relative to the baseline by 741 while increasing doses of vaccine by 556,989. Catch-up vaccination would increase net costs by $10.2million, or $2.38 per person. The incremental cost of HepA vaccine catch-up intervention at age 10years, the midpoint of the ages modeled, was $452,239 per QALY gained. Across age-cohorts, the cost-effectiveness of catch-up vaccination is most favorable at age 12years, resulting in an Incremental Cost-Effectiveness Ratio of $189,000 per QALY gained. Given the low baseline of HAV disease incidence achieved by current vaccination recommendations, our economic model suggests that a catch-up vaccination recommendation would be less cost-effective than many other vaccine interventions, and that HepA catch-up vaccination would become cost effective at a threshold of $50,000 per QALY only when incidence of HAV rises about 5.0 cases per 100,000 population. Copyright © 2016 Elsevier Ltd. All rights reserved.

Self-reported hepatitis A vaccination as a predictor of hepatitis A virus antibody protection in U.S. adults: National Health and Nutrition Examination Survey 2007–2012

PubMed Central

Denniston, Maxine M.; Klevens, R. Monina; Jiles, Ruth B.; Murphy, Trudy V.

2015-01-01

Objectives To estimate the predictive value of self-reported hepatitis A vaccine (HepA) receipt for the presence of hepatitis A virus (HAV) antibody (anti-HAV) from either past infection or vaccination, as an indicator of HAV protection. Methods Using 2007–2012 National Health and Nutrition Examination Survey data, we assigned participants to 4 groups based on self-reported HepA receipt and anti-HAV results. We compared characteristics across groups and calculated three measures of agreement between self-report and serologic status (anti- HAV): percentage concordance, and positive (PPV) and negative (NPV) predictive values. Using logistic regression we investigated factors associated with agreement between self-reported vaccination status and serological results. Results Demographic and other characteristics varied significantly across the 4 groups. Overall agreement between self-reported HepA receipt and serological results was 63.6% (95% confidence interval [CI] 61.9–65.2); PPV and NPV of self-reported vaccination status for serological result were 47.0% (95% CI 44.2–49.8) and 69.4% (95% CI 67.0–71.8), respectively. Mexican American and foreign-born adults had the highest PPVs (71.5% [95% CI 65.9–76.5], and 75.8% [95% CI 71.4–79.7]) and the lowest NPVs (21.8% [95% CI 18.5–25.4], and 20.0% [95% CI 17.2–23.1]), respectively. Young (ages 20–29 years), US-born, and non-Hispanic White adults had the lowest PPVs (37.9% [95% CI 34.5–41.5], 39.1% [95% CI, 36.0–42.3], and 39.8% [36.1–43.7]), and the highest NPVs (76.9% [95% CI 72.2–81.0, 78.5% [95% CI 76.5–80.4)], and 80.6% [95% CI 78.2–82.8), respectively. Multivariate logistic analyses found age, race/ethnicity, education, place of birth and income to be significantly associated with agreement between self-reported vaccination status and serological results. Conclusions When assessing hepatitis A protection, self-report of not having received HepA was most likely to identify persons at risk for hepatitis A infection (no anti-HAV) among young, US-born and non-Hispanic White adults, and self-report of HepA receipt was least likely to be reliable among adults with the same characteristics. PMID:26116252

The Financial Burden of Public Health Responses to Hepatitis A Cases Among Food Handlers, 2012-2014.

PubMed

Morey, Rebecca J; Collier, Melissa G; Nelson, Noele P

When food handlers become ill with hepatitis A virus (HAV) infection, state and local health departments must assess the risk of HAV transmission through prepared food and recommend or provide postexposure prophylaxis (PEP) for those at risk for HAV infection. Providing PEP (eg, hepatitis A [HepA] vaccine or immunoglobulin), however, is costly. To describe the burden of these responses on state and local health departments, we determined the number of public health responses to HAV infections among food handlers by reviewing public internet sources of media articles. We then contacted each health department to collect data on whether PEP was recommended to food handlers or restaurant patrons, the number of PEP doses given, the number of HepA vaccine or immunoglobulin doses given as PEP, and the mean number of health department person-hours required for the response. Of 32 public health responses identified from Twitter, HealthMap, and Google alerts from January 1, 2012, to December 31, 2014, a total of 27 (84%) recommended PEP for other food handlers or restaurant patrons or both. Per public health response, the mean cost per dose of the HepA vaccine or immunoglobulin was $34 139; the mean personnel cost per response was $7329; and the total mean cost of each response was $41 468. PEP is expensive. Less aggressive approaches to PEP, such as limiting PEP to fellow food handlers in nonoutbreak situations, should be considered in the postvaccination era. HepA vaccine for PEP provides long-term immunity and can be used when immunoglobulin is unavailable or cannot be administered within 14 days of exposure to HAV.

Method of treating contaminated HEPA filter media in pulp process

DOEpatents

Hu, Jian S.; Argyle, Mark D.; Demmer, Ricky L.; Mondok, Emilio P.

2003-07-29

A method for reducing contamination of HEPA filters with radioactive and/or hazardous materials is described. The method includes pre-processing of the filter for removing loose particles. Next, the filter medium is removed from the housing, and the housing is decontaminated. Finally, the filter medium is processed as pulp for removing contaminated particles by physical and/or chemical methods, including gravity, flotation, and dissolution of the particles. The decontaminated filter medium is then disposed of as non-RCRA waste; the particles are collected, stabilized, and disposed of according to well known methods of handling such materials; and the liquid medium in which the pulp was processed is recycled.

Flowrate testing of the bag filter LANCS-BOP 6CPVC-1.5-2SPVC (LANCS Industries) at 1 psig

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moore, Murray E.; Currie, Karissa Lyn; Berg, Charlotte Katherine

2016-09-13

The air flowrate through a flexible HEPA grade filter (Part LANCS-BOP 6CPVC-1.5-2SPVC www.lancsindustries.com) was measured at 48 ALPM for a differential pressure drop of 1.0 psig (28 inWC, 7.0 kPa). These filters are rated by the manufacturer to have a flowrate of 3 ALPM at a differential pressure drop of 1 inWC (0.25 kPa). The Los Alamos National Laboratory Aerosol Engineering Facility used one of their test rigs (originally developed to measure the pressure drop in capsule HEPA filters) to measure the airflow through the LANCS bag filter.

Evaluation of environmental filtration control of engineered nanoparticles using the Harvard Versatile Engineered Nanomaterial Generation System (VENGES)

NASA Astrophysics Data System (ADS)

Tsai, Candace S.-J.; Echevarría-Vega, Manuel E.; Sotiriou, Georgios A.; Santeufemio, Christopher; Schmidt, Daniel; Demokritou, Philip; Ellenbecker, Michael

2012-05-01

Applying engineering controls to airborne engineered nanoparticles (ENPs) is critical to prevent environmental releases and worker exposure. This study evaluated the effectiveness of two air sampling and six air cleaning fabric filters at collecting ENPs using industrially relevant flame-made engineered nanoparticles generated using a versatile engineered nanomaterial generation system (VENGES), recently designed and constructed at Harvard University. VENGES has the ability to generate metal and metal oxide exposure atmospheres while controlling important particle properties such as primary particle size, aerosol size distribution, and agglomeration state. For this study, amorphous SiO2 ENPs with a 15.4 nm primary particle size were generated and diluted with HEPA-filtered air. The aerosol was passed through the filter samples at two different filtration face velocities (2.3 and 3.5 m/min). Particle concentrations as a function of particle size were measured upstream and downstream of the filters using a specially designed filter test system to evaluate filtration efficiency. Real time instruments (FMPS and APS) were used to measure particle concentration for diameters from 5 to 20,000 nm. Membrane-coated fabric filters were found to have enhanced nanoparticle collection efficiency by 20-46 % points compared to non-coated fabric and could provide collection efficiency above 95 %.

Evaluation of environmental filtration control of engineered nanoparticles using the Harvard Versatile Engineered Nanomaterial Generation System (VENGES)

PubMed Central

Echevarría-Vega, Manuel E.; Sotiriou, Georgios A.; Santeufemio, Christopher; Schmidt, Daniel; Demokritou, Philip; Ellenbecker, Michael

2013-01-01

Applying engineering controls to airborne engineered nanoparticles (ENPs) is critical to prevent environmental releases and worker exposure. This study evaluated the effectiveness of two air sampling and six air cleaning fabric filters at collecting ENPs using industrially relevant flame-made engineered nanoparticles generated using a versatile engineered nanomaterial generation system (VENGES), recently designed and constructed at Harvard University. VENGES has the ability to generate metal and metal oxide exposure atmospheres while controlling important particle properties such as primary particle size, aerosol size distribution, and agglomeration state. For this study, amorphous SiO2 ENPs with a 15.4 nm primary particle size were generated and diluted with HEPA-filtered air. The aerosol was passed through the filter samples at two different filtration face velocities (2.3 and 3.5 m/min). Particle concentrations as a function of particle size were measured upstream and downstream of the filters using a specially designed filter test system to evaluate filtration efficiency. Real time instruments (FMPS and APS) were used to measure particle concentration for diameters from 5 to 20,000 nm. Membrane-coated fabric filters were found to have enhanced nanoparticle collection efficiency by 20–46 % points compared to non-coated fabric and could provide collection efficiency above 95 %. PMID:23412707

Development of a Cryostat to Characterize Nano-scale Superconducting Quantum Interference Devices

NASA Astrophysics Data System (ADS)

Longo, Mathew; Matheny, Matthew; Knudsen, Jasmine

2016-03-01

We have designed and constructed a low-noise vacuum cryostat to be used for the characterization of nano-scale superconducting quantum interference devices (SQUIDs). Such devices are very sensitive to magnetic fields and can measure changes in flux on the order of a single electron magnetic moment. As a part of the design process, we calculated the separation required between the cryogenic preamplifier and superconducting magnet, including a high-permeability magnetic shield, using a finite-element model of the apparatus. The cryostat comprises a vacuum cross at room temperature for filtered DC and shielded RF electrical connections, a thin-wall stainless steel support tube, a taper-sealed cryogenic vacuum can, and internal mechanical support and wiring for the nanoSQUID. The Dewar is modified with a room-temperature flange with a sliding seal for the cryostat. The flange supports the superconducting 3 Tesla magnet and thermometry wiring. Upon completion of the cryostat fabrication and Dewar modifications, operation of the nanoSQUIDs as transported from our collaborator's laboratory in Israel will be confirmed, as the lead forming the SQUID is sensitive to oxidation and the SQUIDs must be shipped in a vacuum container. After operation of the nanoSQUIDs is confirmed, the primary work of characterizing their high-speed properties will begin. This will include looking at the measurement of relaxation oscillations at high bandwidth in comparison to the theoretical predictions of the current model.

Cost-effectiveness analysis of catch-up hepatitis A vaccination among unvaccinated/partially-vaccinated children

PubMed Central

Hankin-Wei, Abigail; Rein, David B.; Hernandez-Romieu, Alfonso; Kennedy, Mallory J.; Bulkow, Lisa; Rosenberg, Eli; Trigg, Monica; Nelson, Noele P.

2017-01-01

Background Since 2006, the US Centers for Disease Control and Prevention has recommended hepatitis A (HepA) vaccination routinely for children aged 12–23 months to prevent hepatitis A virus (HAV) infection. However, a substantial proportion of US children are unvaccinated and susceptible to infection. We present results of economic modeling to assess whether a one-time catch-up HepA vaccination recommendation would be cost-effective. Methods We developed a Markov model of HAV infection that followed a single cohort from birth through death (birth to age 95 years). The model compared the health and economic outcomes from catch-up vaccination interventions for children at target ages from two through 17 years vs. outcomes resulting from maintaining the current recommendation of routine vaccination at age one year with no catch-up intervention. Results Over the lifetime of the cohort, catch-up vaccination would reduce the total number of infections relative to the baseline by 741 while increasing doses of vaccine by 556,989. Catch-up vaccination would increase net costs by $10.2 million, or $2.38 per person. The incremental cost of HepA vaccine catch-up intervention at age 10 years, the midpoint of the ages modeled, was $452,239 per QALY gained. Across age-cohorts, the cost-effectiveness of catch-up vaccination is most favorable at age 12 years, resulting in an Incremental Cost-Effectiveness Ratio of $189,000 per QALY gained. Conclusions Given the low baseline of HAV disease incidence achieved by current vaccination recommendations, our economic model suggests that a catch-up vaccination recommendation would be less cost-effective than many other vaccine interventions, and that HepA catch-up vaccination would become cost effective at a threshold of $50,000 per QALY only when incidence of HAV rises about 5.0 cases per 100,000 population. PMID:27317459

The use of high-efficiency particulate air-filter respirators to protect hospital workers from tuberculosis. A cost-effectiveness analysis.

PubMed

Adal, K A; Anglim, A M; Palumbo, C L; Titus, M G; Coyner, B J; Farr, B M

1994-07-21

After outbreaks of multidrug-resistant tuberculosis, the Centers for Disease Control and Prevention proposed the use of respirators with high-efficiency particulate air filters (HEPA respirators) as part of isolation precautions against tuberculosis, along with a respiratory-protection program for health care workers that includes medical evaluation, training, and tests of the fit of the respirators. Each HEPA respirator costs between $7.51 and $9.08, about 10 times the cost of respirators currently used. We conducted a cost-effectiveness analysis using data from the University of Virginia Hospital on exposure to patients with tuberculosis and rates at which the purified-protein-derivative (PPD) skin test became positive in hospital workers. The costs of a respiratory-protection program were based on those of an existing program for workers dealing with hazardous substances. During 1992, 11 patients with documented tuberculosis were admitted to our hospital. Eight of 3852 workers (0.2 percent) had PPD tests that became positive. Five of these conversions were believed to be due to the booster phenomenon; one followed unprotected exposure to a patient not yet in isolation; the other two occurred in workers who had never entered a tuberculosis isolation room. These data suggest that it will take more than one year for the use of HEPA respirators to prevent a single conversion of the PPD test. Assuming that one conversion is prevented per year, however, it would take 41 years at out hospital to prevent one case of occupationally acquired tuberculosis, at a cost of $1.3 million to $18.5 million. Given the effectiveness of currently recommended measures to prevent nosocomial transmission of tuberculosis, the addition of HEPA respirators would offer negligible protective efficacy at great cost.

Effect of heat and moisture exchanger (HME) positioning on inspiratory gas humidification.

PubMed

Inui, Daisuke; Oto, Jun; Nishimura, Masaji

2006-08-08

In mechanically ventilated patients, we investigated how positioning the heat and moisture exchanger (HME) at different places on the ventilator circuit affected inspiratory gas humidification. Absolute humidity (AH) and temperature (TEMP) at the proximal end of endotracheal tube (ETT) were measured in ten mechanically ventilated patients. The HME was connected either directly proximal to the ETT (Site 1) or at before the circuit Y-piece (Site 2: distance from proximal end of ETT and Site 2 was about 19 cm) (Figure. 1). Two devices, Hygrobac S (Mallinckrodt Dar, Mirandola, Italy) and Thermovent HEPA (Smiths Medical International Ltd., Kent, UK) were tested. AH and TEMP were measured with a hygrometer (Moiscope, MERA Co., Ltd., Tokyo, Japan). Hygrobac S provided significantly higher AH and TEMP at both sites than Thermovent HEPA. Both Hygrobac S and with Thermovent HEPA provided significantly higher AH and TEMP when placed proximally to the ETT. Although placement proximal to the ETT improved both AH and TEMP in both HMEs tested, one HME performed better in the distal position than the other HME in the proximal position. We conclude the both the type and placement of HME can make a significant difference in maintaining AH and TEMP during adult ventilation.

Chemical and thermal stability of the characteristics of filtered vacuum arc deposited ZnO, SnO2 and zinc stannate thin films

NASA Astrophysics Data System (ADS)

Çetinörgü, E.; Goldsmith, S.

2007-09-01

ZnO, SnO2 and zinc stannate thin films were deposited on commercial microscope glass and UV fused silica substrates using filtered vacuum arc deposition system. During the deposition, the substrate temperature was at room temperature (RT) or at 400 °C. The film structure and composition were determined using x-ray diffraction and x-ray photoelectron spectroscopy, respectively. The transmission of the films in the VIS was 85% to 90%. The thermal stability of the film electrical resistance was determined in air as a function of the temperature in the range 28 °C (RT) to 200 °C. The resistance of ZnO increased from ~ 5000 to 105 Ω when heated to 200 °C, that of SnO2 films increased from 500 to 3900 Ω, whereas that of zinc stannate thin films increased only from 370 to 470 Ω. During sample cooling to RT, the resistance of ZnO and SnO2 thin films continued to rise considerably; however, the increase in the zinc stannate thin film resistance was significantly lower. After cooling to RT, ZnO and SnO2 thin films became practically insulators, while the resistance of zinc stannate was 680 Ω. The chemical stability of the films was determined by immersing in acidic and basic solutions up to 27 h. The SnO2 thin films were more stable in the HCl solution than the ZnO and the zinc stannate thin films; however, SnO2 and zinc stannate thin films that were immersed in the NaOH solution did not dissolve after 27 h.

Investigation of the influence of the zeta-potential on the filtration rate in the presence of collectors

NASA Technical Reports Server (NTRS)

Provirnina, E. V.; Barbin, M. B.

1984-01-01

The value of the zeta-potential does not have an explicit effect, which is expressed by a simple math correlation, on filtration rate when a solution of the tested collector is filtered through a cake prepared under standard conditions from the examined particulate material. The zeta-potential measurements and filtration tests were carried out on silica and galena with solutions contg. a cationic container ANP and Et xanthane, resp. at PH = 6.5, varying concentration of the agent (0-2500 g/ton), and under a vacuum of 100 to 600 mm Hg.

Effect of heat and moisture exchanger (HME) positioning on inspiratory gas humidification

PubMed Central

Inui, Daisuke; Oto, Jun; Nishimura, Masaji

2006-01-01

Background In mechanically ventilated patients, we investigated how positioning the heat and moisture exchanger (HME) at different places on the ventilator circuit affected inspiratory gas humidification. Methods Absolute humidity (AH) and temperature (TEMP) at the proximal end of endotracheal tube (ETT) were measured in ten mechanically ventilated patients. The HME was connected either directly proximal to the ETT (Site 1) or at before the circuit Y-piece (Site 2: distance from proximal end of ETT and Site 2 was about 19 cm) (Figure. 1). Two devices, Hygrobac S (Mallinckrodt Dar, Mirandola, Italy) and Thermovent HEPA (Smiths Medical International Ltd., Kent, UK) were tested. AH and TEMP were measured with a hygrometer (Moiscope, MERA Co., Ltd., Tokyo, Japan). Results Hygrobac S provided significantly higher AH and TEMP at both sites than Thermovent HEPA. Both Hygrobac S and with Thermovent HEPA provided significantly higher AH and TEMP when placed proximally to the ETT. Conclusion Although placement proximal to the ETT improved both AH and TEMP in both HMEs tested, one HME performed better in the distal position than the other HME in the proximal position. We conclude the both the type and placement of HME can make a significant difference in maintaining AH and TEMP during adult ventilation. PMID:16895607

Physical activity and other lifestyle behaviors in a Portuguese sample of adults: results from the Azorean Physical Activity and Health Study.

PubMed

Santos, Rute; Santos, Maria Paula; Ribeiro, José Carlos; Mota, Jorge

2009-11-01

The aims of this study were to describe physical activity (PA) prevalence and compare it with other countries and to investigate possible associations between PA and other lifestyle behaviors in Azorean adults. 9991 adults (5723 women), aged 37.8 +/- 9.5 years, of the 2004 Azorean Physical Activity and Health Study. IPAQ assessed PA. All other lifestyle behaviors (age, gender, education level, income, employment, marital status, number of children, meal frequency, sleep time, sitting time, body mass index and alcohol and tobacco consumptions) were also self-reported. 57.1% of the participants met current PA recommendations and 32.2% were categorized as Health Enhancing PA (HEPA). Women were less likely to achieve PA recommendations, as well as the HEPA level. In both genders, higher education level, employment status, higher income, and sitting for more than 3h/day were negative predictors of HEPA; and, having at least 5 meals/day was positive predictor for the same PA level. There is a significant proportion of Azoreans, particularly women, that does not do enough PA. Targeted programs for Azoreans aimed to increase PA levels should pay special attention on women, and consider a multifactorial approach, once several lifestyle behaviors seem to interact with PA levels, in this population.

Direct-current cathodic vacuum arc system with magnetic-field mechanism for plasma stabilization.

PubMed

Zhang, H-S; Komvopoulos, K

2008-07-01

Filtered cathodic vacuum arc (FCVA) deposition is characterized by plasma beam directionality, plasma energy adjustment via substrate biasing, macroparticle filtering, and independent substrate temperature control. Between the two modes of FCVA deposition, namely, direct current (dc) and pulsed arc, the dc mode yields higher deposition rates than the pulsed mode. However, maintaining the dc arc discharge is challenging because of its inherent plasma instabilities. A system generating a special configuration of magnetic field that stabilizes the dc arc discharge during film deposition is presented. This magnetic field is also part of the out-of-plane magnetic filter used to focus the plasma beam and prevent macroparticle film contamination. The efficiency of the plasma-stabilizing magnetic-field mechanism is demonstrated by the deposition of amorphous carbon (a-C) films exhibiting significantly high hardness and tetrahedral carbon hybridization (sp3) contents higher than 70%. Such high-quality films cannot be produced by dc arc deposition without the plasma-stabilizing mechanism presented in this study.

Infiltration of forest fire and residential wood smoke: an evaluation of air cleaner effectiveness.

PubMed

Barn, Prabjit; Larson, Timothy; Noullett, Melanie; Kennedy, Susan; Copes, Ray; Brauer, Michael

2008-09-01

Communities impacted by fine-particle air pollution (particles with an aerodynamic diameter less than 2.5 microm; PM(2.5)) from forest fires and residential wood burning require effective, evidence-based exposure-reduction strategies. Public health recommendations during smoke episodes typically include advising community members to remain indoors and the use of air cleaners, yet little information is available on the effectiveness of these measures. Our study attempted to address the following objectives: to measure indoor infiltration factor (F(inf)) of PM(2.5) from forest fires/wood smoke, to determine the effectiveness of high-efficiency particulate air (HEPA) filter air cleaners in reducing indoor PM(2.5), and to analyze the home determinants of F(inf) and air cleaner effectiveness (ACE). We collected indoor/outdoor 1-min PM(2.5) averages and 48-h outdoor PM(2.5) filter samples for 21 winter and 17 summer homes impacted by wood burning and forest fire smoke, respectively, during 2004-2005. A portable HEPA filter air cleaner was operated indoors with the filter removed for one of two sampling days. Particle F(inf) and ACE were calculated for each home using a recursive model. We found mean F(inf)+/-SD was 0.27+/-0.18 and 0.61+/-0.27 in winter (n=19) and summer (n=13), respectively, for days when HEPA filters were not used. Lower F(inf)+/-SD values of 0.10+/-0.08 and 0.19+/-0.20 were found on corresponding days when HEPA filters were in place. Mean+/-SD ACE ([F(inf) without filter-F(inf) with filter]/F(inf) without filter) in winter and summer were 55+/-38% and 65+/-35%, respectively. Number of windows and season predicted F(inf) (P<0.001). No significant predictors of ACE were identified. Our findings show that remaining indoors combined with use of air cleaner can effectively reduce PM(2.5) exposure during forest fires and residential wood burning.

Magnetically Filtered Faraday Probe for Measuring the Ion Current Density Profile of a Hall Thruster

DTIC Science & Technology

2006-01-01

Hall thruster is investigated. The MFFP is designed to eliminate the collection of low-energy, charge-exchange (CEX) ions by using a variable magnetic field as an ion filter. In this study, a MFFP, Faraday probe with a reduced acceptance angle (BFP), and nude Faraday probe are used to measure the ion current density profile of a 5 kW Hall thruster operating over the range of 300-500 V and 5-10 mg/s. The probes are evaluated on a xenon propellant Hall thruster in the University of Michigan Large Vacuum Test Facility at operating

Bacterial communities in commercial aircraft high-efficiency particulate air (HEPA) filters assessed by PhyloChip analysis.

PubMed

Korves, T M; Piceno, Y M; Tom, L M; Desantis, T Z; Jones, B W; Andersen, G L; Hwang, G M

2013-02-01

Air travel can rapidly transport infectious diseases globally. To facilitate the design of biosensors for infectious organisms in commercial aircraft, we characterized bacterial diversity in aircraft air. Samples from 61 aircraft high-efficiency particulate air (HEPA) filters were analyzed with a custom microarray of 16S rRNA gene sequences (PhyloChip), representing bacterial lineages. A total of 606 subfamilies from 41 phyla were detected. The most abundant bacterial subfamilies included bacteria associated with humans, especially skin, gastrointestinal and respiratory tracts, and with water and soil habitats. Operational taxonomic units that contain important human pathogens as well as their close, more benign relatives were detected. When compared to 43 samples of urban outdoor air, aircraft samples differed in composition, with higher relative abundance of Firmicutes and Gammaproteobacteria lineages in aircraft samples, and higher relative abundance of Actinobacteria and Betaproteobacteria lineages in outdoor air samples. In addition, aircraft and outdoor air samples differed in the incidence of taxa containing human pathogens. Overall, these results demonstrate that HEPA filter samples can be used to deeply characterize bacterial diversity in aircraft air and suggest that the presence of close relatives of certain pathogens must be taken into account in probe design for aircraft biosensors. A biosensor that could be deployed in commercial aircraft would be required to function at an extremely low false alarm rate, making an understanding of microbial background important. This study reveals a diverse bacterial background present on aircraft, including bacteria closely related to pathogens of public health concern. Furthermore, this aircraft background is different from outdoor air, suggesting different probes may be needed to detect airborne contaminants to achieve minimal false alarm rates. This study also indicates that aircraft HEPA filters could be used with other molecular techniques to further characterize background bacteria and in investigations in the wake of a disease outbreak. © 2012 John Wiley & Sons A/S.

Results from Evaluation of Representative ASME AG-1 Section FK Radial Flow Dimple Pleated HEPA Filters Under Elevated Conditions - 12002

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giffin, Paxton K.; Parsons, Michael S.; Rickert, Jaime G.

The American Society of Mechanical Engineers (ASME) has recently added Section FK establishing requirements for radial flow HEPA filters to the Code on Nuclear Air and Gas Treatment (AG-1). Section FK filters are expected to be a major element in the HEPA filtration systems across the US Department of Energy (DOE) complex. Radial flow filters have been used in Europe for some time, however a limited amount of performance evaluation data exists with respect to these new AG-1 Section FK units. In consultation with a technical working group, the Institute for Clean Energy Technology (ICET) at Mississippi State University (MSU)hasmore » evaluated a series of representative AG-1 Section FK dimple pleated radial flow HEPA filters. The effects of elevated relative humidity and temperature conditions on these filters are particularly concerning. Results from the evaluation of Section FK filters under ambient conditions have been presented at the 2011 waste management conference. Additions to the previous test stand to enable high temperature and high humidity testing, a review of the equipment used, the steps taken to characterize the new additions, and the filter test results are presented in this study. Test filters were evaluated at a volumetric flow rate of 56.6 m{sup 3}/min (2000 cfm) and were challenged under ambient conditions with Alumina, Al(OH){sub 3}, until reaching a differential pressure of 1 kPa (4 in. w.c.), at which time the filters were tested, unchallenged with aerosol, at 54 deg. C (130 deg. F) for approximately 1 hour. At the end of that hour water was sprayed near the heat source to maximize vaporization exposing the filter to an elevated relative humidity up to 95%. Collected data include differential pressure, temperature, relative humidity, and volumetric flow rate versus time. (authors)« less

Murine hepatic aldehyde dehydrogenase 1a1 is a major contributor to oxidation of aldehydes formed by lipid peroxidation

PubMed Central

Makia, Ngome L.; Bojang, Pasano; Falkner, K. Cameron; Conklin, Daniel J.; Prough, Russell A.

2015-01-01

Reactive lipid aldehydes are implicated in the pathogenesis of various oxidative stress-mediated diseases, including non-alcoholic steatohepatitis, atherosclerosis, Alzheimer’s and cataract. In the present study, we sought to define which hepatic Aldh isoform plays a major role in detoxification of lipid-derived aldehydes, such as acrolein and HNE by enzyme kinetic and gene expression studies. The catalytic efficiencies for metabolism of acrolein by Aldh1a1 was comparable to that of Aldh3a1 (Vmax/Km = 23). However, Aldh1a1 exhibits far higher affinity for acrolein (Km = 23.2 μM) compared to Aldh3a1 (Km = 464 μM). Aldh1a1 displays a 3-fold higher catalytic efficiency for HNE than Aldh3a1 (218 vs 69 ml/min/mg). The endogenous Aldh1a1 gene was highly expressed in mouse liver and a liver-derived cell line (Hepa-1c1c7) compared to Aldh2, Aldh1b1 and Aldh3a1. Aldh1a1 mRNA levels was 34-fold and 73-fold higher than Aldh2 in mouse liver and Hepa-1c1c7 cells respectively. Aldh3a1 gene was absent in mouse liver, but moderately expressed in Hepa-1c1c7 cells compared to Aldh1a1. We demonstrated that knockdown of Aldh1a1 expression by siRNA caused Hepa-1c1c7 cells to be more sensitive to acrolein-induced cell death and resulted in increased accumulation of acrolein-protein adducts and caspase 3 activation. These results indicate that Aldh1a1 plays a major role in cellular defense against oxidative damage induced by reactive lipid aldehydes in mouse liver. We also noted that hepatic Aldh1a1 mRNA levels were significantly increased (≈ 3 fold) in acrolein-fed mice compared to control. In addition, hepatic cytosolic ALDH activity was induced by acrolein when 1 mM NAD+ was used as cofactor, suggesting an Aldh1a1-protective mechanism against acrolein toxicity in mice liver. Thus, mechanisms to induce Aldh1a1 gene expression may provide a useful rationale for therapeutic protection against oxidative stress-induced pathologies. PMID:21256123

Vacuum distillation: vapor filtered-catalytic oxidation water reclamation system utilizing radioisotopes

NASA Technical Reports Server (NTRS)

Honegger, R. J.; Remus, G. A.; Kurg, E. K.

1971-01-01

The development of a functional model water reclamation system is discussed. The system produces potable water by distillation from the urine and respiration-perspiration condensate at the normal rate generated by four men. Basic processes employed are vacuum distillation, vapor filtration, vapor phase catalytic oxidation, and condensation. The system is designed to use four 75-watt isotope heaters for distillation thermal input, and one 45-watt isotope for the catalytic oxidation unit. The system is capable of collecting and storing urine, and provides for stabilizing the urine by chemical pretreatment. The functional model system is designed for operation in a weightless condition with liquid-vapor phase separators for the evaporator still, and centrifugal separators for urine collection and vapor condensation. The system provides for storing and dispensing reclaimed potable water. The system operates in a batch mode for 40 days, with urine residues accumulating in the evaporator. The evaporator still and residue are removed to storage and replaced with a fresh still for the next 40-day period.

U-235 Holdup Measurements in the 321-M Lathe HEPA Banks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Salaymeh, S.R.

The Analytical Development Section of Savannah River Technology Center (SRTC) was requested by the Facilities Decommissioning Division (FDD) to determine the holdup of enriched uranium in the 321-M facility as part of an overall deactivation project of the facility. The results of the holdup assays are essential for determining compliance with the Waste Acceptance Criteria, Material Control and Accountability, and to meet criticality safety controls. This report covers holdup measurements of uranium residue in six high efficiency particulate air (HEPA) filter banks of the A-lathe and B-lathe exhaust systems of the 321-M facility. This report discusses the non-destructive assay measurements,more » assumptions, calculations, and results of the uranium holdup in these six items.« less

40 CFR 721.10002 - 2-Thiazolidinone.

Code of Federal Regulations, 2011 CFR

2011-07-01

... HEPA filters; and supplied-air respirator operated in pressure demand or continuous flow mode and... substance may cause internal organ effects (blood, liver, and kidney). The substance may cause developmental...

Spectral Characterizations of the Clouds and the Earth's Radiant Energy System (CERES) Thermistor Bolometers using Fourier Transform Spectrometer (FTS) Techniques

NASA Technical Reports Server (NTRS)

Thornhill, K. Lee; Bitting, Herbert; Lee, Robert B., III; Paden, Jack; Pandey, Dhirendra K.; Priestley, Kory J.; Thomas, Susan; Wilson, Robert S.

1998-01-01

Fourier Transform Spectrometer (FTS) techniques are being used to characterize the relative spectral response, or sensitivity, of scanning thermistor bolometers in the infrared (IR) region (2 - >= 100-micrometers). The bolometers are being used in the Clouds and the Earth's Radiant Energy System (CERES) program. The CERES measurements are designed to provide precise, long term monitoring of the Earth's atmospheric radiation energy budget. The CERES instrument houses three bolometric radiometers, a total wavelength (0.3- >= 150-micrometers) sensor, a shortwave (0.3-5-micrometers) sensor, and an atmospheric window (8-12-micrometers) sensor. Accurate spectral characterization is necessary for determining filtered radiances for longwave radiometric calibrations. The CERES bolometers spectral response's are measured in the TRW FTS Vacuum Chamber Facility (FTS - VCF), which uses a FTS as the source and a cavity pyroelectric trap detector as the reference. The CERES bolometers and the cavity detector are contained in a vacuum chamber, while the FTS source is housed in a GN2 purged chamber. Due to the thermal time constant of the CERES bolometers, the FTS must be operated in a step mode. Data are acquired in 6 IR spectral bands covering the entire longwave IR region. In this paper, the TRW spectral calibration facility design and data measurement techniques are described. Two approaches are presented which convert the total channel FTS data into the final CERES spectral characterizations, producing the same calibration coefficients (within 0.1 percent). The resulting spectral response curves are shown, along with error sources in the two procedures. Finally, the impact of each spectral response curve on CERES data validation will be examined through analysis of filtered radiance values from various typical scene types.

40 CFR 721.10002 - 2-Thiazolidinone.

Code of Federal Regulations, 2010 CFR

2010-07-01

... HEPA filters; and supplied-air respirator operated in pressure demand or continuous flow mode and... substance may cause internal organ effects (blood, liver, and kidney). The substance may cause developmental...

Determination of selenium at trace levels in geologic materials by energy-dispersive X-ray fluorescence spectrometry

USGS Publications Warehouse

Wahlberg, J.S.

1981-01-01

Low levels of selenium (0.1-500 ppm) in both organic and inorganic geologic materials can be semiquantitatively measured by isolating Se as a thin film for presentation to an energy-dispersive X-ray fluorescence spectrometer. Suitably pulverized samples are first digested by fusing with a mixture of Na2CO3 and Na2O2. The fusion cake is dissolved in distilled water, buffered with NH4Cl, and filtered to remove Si and the R2O3 group. A carrier solution of Na2TeO4, plus solid KI, hydrazine sulfate and Na2SO3, is added to the filtrate. The solution is then vacuum-filtered through a 0.45-??m pore-size filter disc. The filter, with the thin film of precipitate, is supported between two sheets of Mylar?? film for analysis. Good agreement is shown between data reported in this study and literature values reported by epithermal neutron-activation analysis and spectrofluorimetry. The method can be made quantitative by utilizing a secondary precipitation to assure complete recovery of the Se. The X-ray method offers fast turn-around time and a reasonably high production rate. ?? 1981.

Plasma response measurements of non-axisymmetric magnetic perturbations on DIII-D via soft x-ray imaging

DOE PAGES

Shafer, Morgan W.; Unterberg, Ezekial A.; Wingen, Andreas; ...

2014-12-29

Recent observations on DIII-D have advanced the understanding of plasma response to applied resonant magnetic perturbations (RMPs) in both H-mode and L-mode plasmas. Three distinct 3D features localized in minor radius are imaged via filtered soft x-ray emission: (i) the formation of lobes extending from the unperturbed separatrix in the X-point region at the plasma boundary, (ii) helical kink-like perturbations in the steep-gradient region inside the separatrix, and (iii) amplified islands in the core of a low-rotation L-mode plasma. In this study, these measurements are used to test and to validate plasma response models, which are crucial for providing predictivemore » capability of edge-localized mode control. In particular, vacuum and two-fluid resistive magnetohydrodynamic(MHD) responses are tested in the regions of these measurements. At the plasma boundary in H-mode discharges with n = 3 RMPs applied, measurements compare well to vacuum-field calculations that predict lobe structures. Yet in the steep-gradient region, measurements agree better with calculations from the linear resistive two-fluid MHD code, M3D-C1. Relative to the vacuum fields, the resistive two-fluid MHD calculations show a reduction in the pitch-resonant components of the normal magnetic field (screening), and amplification of non-resonant components associated with ideal kink modes. However, the calculations still over-predict the amplitude of the measuredperturbation by a factor of 4. In a slowly rotating L-mode plasma with n = 1 RMPs, core islands are observed amplified from vacuum predictions. Finally, these results indicate that while the vacuum approach describes measurements in the edge region well, it is important to include effects of extended MHD in the pedestal and deeper in the plasma core.« less

The National Asthma Campaign Manchester Asthma and Allergy Study.

PubMed

Custovic, Adnan; Simpson, Bridget M; Murray, Clare S; Lowe, Lesley; Woodcock, Ashley

2002-01-01

The NACManchester Asthma and Allergy Study is a prospective study of the development of asthma and allergies in childhood. The subjects (995 children at age 3 years) were recruited in utero by screening parents in the antenatal clinic using skin prick testing and a questionnaire regarding allergic diseases. Children were assigned to risk groups according to parental atopic status (high risk, both parents atopic; medium risk, one parent atopic; low risk, neither parent atopic). A subgroup of those at high risk (with no pets in the home) was randomized to stringent environmental control (allergen impermeable covers for the parental and infant bed, hot washing of bedding weekly, HEPA vacuum cleaner, hard floor for the nursery), and the remainder followed a normal regime. The children have been followed prospectively. The environmental influences are very clearly defined. Measurements of environmental exposures include levels of house dust mite; cat and dog allergens during pregnancy and early life; pet ownership and exposure; childcare arrangements; number of siblings; vaccination uptake; thorough dietary questionnaire; and endotoxin exposure. Further unique objective outcome in the cohort is the assessment of lung function in preschool children using specific airways resistance, which at age 3 years clearly reflects both genetic and environmental influences.

Particulate generation and control in the PREPP (Process Experimental Pilot Plant) incinerator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stermer, D.L.; Gale, L.G.

1989-03-01

Particulate emissions in radioactive incineration systems using a wet scrubbing system are generally ultimately controlled by flowing the process offgas stream through a high-efficiency filter, such as a High Efficient Particulate Air (HEPA) filter. Because HEPA filters are capable of reducing particulate emissions over an order of magnitude below regulatory limits, they consequently are vulnerable to high loading rates. This becomes a serious handicap in radioactive systems when filter change-out is required at an unacceptably high rate. The Process Experimental Pilot Plant (PREPP) incineration system is designed for processing retrieved low level mixed hazardous waste. It has a wet offgasmore » treatment system consisting of a Quencher, Venturi Scrubber, Entrainment Eliminator, Mist Eliminator, two stages of HEPA filters, and induced draft fans. During previous tests, it was noted that the offgas filters loaded with particulate at a rate requiring replacement as often as every four hours. During 1988, PREPP conducted a series of tests which included an investigation of the causes of heavy particulate accumulation on the offgas filters in relation to various operating parameters. This was done by measuring the particulate concentrations in the offgas system, primarily as a function of scrub solution salt concentration, waste feed rate, and offgas flow rate. 2 figs., 9 tabs.« less

Walking in the high-rise city: a Health Enhancement and Pedometer-determined Ambulatory (HEPA) program in Hong Kong

PubMed Central

Leung, Angela YM; Cheung, Mike KT; Tse, Michael A; Shum, Wai Chuen; Lancaster, BJ; Lam, Cindy LK

2014-01-01

Due to the lack of good infrastructure in the public estates, many older adults in urban areas are sedentary. The Health Enhancement and Pedometer-Determined Ambulatory (HEPA) program was developed to assist older adults with diabetes and/or hypertension to acquire walking exercise habits and to build social support, while engaged in regular physical activity. This study aimed to describe the HEPA program and to report changes in participants’ walking capacity and body strength after 10-week walking sessions. A pre- and postintervention design was used. Pedometers were used to measure the number of steps taken per day before and after the 10-week intervention. Upper and lower body strength, lower body flexibility, and quality of life were assessed. A total of 205 older adults completed the program and all health assessments. After the 10-week intervention, the average number of steps per day increased by 36%, from 6,591 to 8,934. Lower body strength, upper body strength, and aerobic fitness increased significantly after 10 weeks, along with improvement in the 12-item Short Form Health Survey (SF™-12) physical and mental health component summary scores. A social support network was built in the neighborhood, and the local environment was utilized to make walking possible and enjoyable. PMID:25170259

PubMed Central

SPAGNOLO, A.M.; OTTRIA, G.; AMICIZIA, D.; PERDELLI, F.

2013-01-01

Summary Surgical site infections (SSI) account for 14% to 17% of all hospital-acquired infections and 38% of nosocomial infections in surgical patients. SSI remain a substantial cause of morbidity and death, possibly because of the larger numbers of elderly surgical patients or those with a variety of chronic and immunocompromising conditions, and emergence of antibiotic-resistant microorganisms. Factors causing surgical site infection are multifarious. Several studies have identified the main patient-related (endogenous risk factors) and procedure-related (external risk factors) factors that influence the risk of SSI. The rate of surgical wound infections is strongly influenced by operating theatre quality, too. A safe and salubrious operating theatre is an environment in which all sources of pollution and any micro-environmental alterations are kept strictly under control. This can be achieved only through careful planning, maintenance and periodic checks, as well as proper ongoing training for staff. Many international scientific societies have produced guidelines regarding the environmental features of operating theatres (positive pressure, exchanges of filtered air per hour, air-conditioning systems with HEPA filters, etc.) and issued recommendations on healthcare-associated infection, including SSI, concerning surveillance methods, intervention to actively prevent SSI and approaches to monitoring the implementation of such strategies. Therefore, the prevention of SSI requires a multidisciplinary approach and the commitment of all concerned, including that of those who are responsible for the design, layout and functioning of operating theatres. PMID:24783890

THE EFFECT OF SAMe AND BETAINE ON HEPA 1-6, C34 AND E47 LIVER CELL SURVIVAL IN VITRO

PubMed Central

Oliva, Joan; Zhong, Jin; Buslon, Virgil S.; French, Samuel W.

2011-01-01

In recent years, methyl one-carbon metabolism has received a great deal of attention because the disruption of methyl balance in a variety of genetically modified mice is associated with the development of various forms of liver injury, namely fatty liverdisease and hepatocellular carcinoma (HCC). In addition, patients with liver disease often have an abnormal expression of key genes involved in methionine metabolism as well as elevated serum levels of methionine and homocysteine (Hcy). S-adenosylmethionine (SAMe) has rapidly moved from being a methyl donor to a key metabolite that regulates hepatocyte proliferation, necrosis and differentiation. Biosynthesis of SAMe occurs in all mammalian cells as the first step in methionine catabolism in a reaction catalyzed by methionine adenosyltransferase (MAT). Decreased hepatic SAMe biosynthesis is a consequence of numerous forms of chronic liver injury. In an animal model of chronic liver SAMe deficiency, the liver is predisposed to further injury and develops spontaneous steatohepatitis and HCC. SAMe treatment in experimental animal models of liver injury shows that its hepatoprotective properties. Meta-analyses also showed that it is effective in the treatment of patients with cholestatic liver diseases. We studied the survival of liver cells treated with SAMe and betaine using Hepa 1–6 and E47/C34 cell lines. We showed that exogenous SAMe decreased the number of Hepa 1–6 and E47/C34 cells, and increased the number of dead cells in vitro. Betaine had no significant effect on the number of surviving cells and the number of dead cells. The combination of both methyl donors significantly increased the survival of liver cells and reduced necrosis, compare to SAMe alone. This study showed the inhibition of the proliferatino and increased necrosis in response to SAMe on liver cancer cell lines Hepa 1–6 and C34. PMID:22032937

Nuclear air cleaning: the need for a change in emphasis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carbaugh, E.H.

1982-11-01

The nuclear industry now has over 35 years of experience in nuclear air cleaning. This experience covers technology development, system design, operations, and maintenance. Much of the past experience has been directed towards technology development with particular emphasis on high efficiency particulate air (HEPA) filters. Implementation of this technology has lagged its development by a number of years. A recent study examines the cause and frequencies of HEPA filter changeouts and failures. These data lead to a conclusion that a shift in emphasis from technology development to the training of personnel and the designing and maintaining of such systems ismore » needed. Some highlights of the data and a discussion of topics which should be addressed in training will be presented.« less

Solid and Liquid Waste Drying Bag

NASA Technical Reports Server (NTRS)

Litwiller, Eric (Inventor); Hogan, John A. (Inventor); Fisher, John W. (Inventor)

2009-01-01

Method and system for processing waste from human activities, including solids, liquids and vapors. A fluid-impermeable bag, lined with a liquid-impermeable but vapor-permeable membrane, defining an inner bag, is provided. A vacuum force is provided to extract vapors so that the waste is moved toward a selected region in the inner bag, extracted vapors, including the waste vapors and vaporized portions of the waste liquids are transported across the membrane, and most or all of the solids remain within the liner. Extracted vapors are filtered, and sanitized components thereof are isolated and optionally stored. The solids remaining within the liner are optionally dried and isolated for ultimate disposal.

77 FR 37000 - Marine Mammals; File No. 814-1899

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-20

... hereby given that the North Slope Borough Department of Wildlife Management, P.O. Box 69, Barrow, AK 99723 [Taqulik Hepa, Responsible Party; Dr. John C. George, Principal Investigator], has been issued a...

Monitoring and reducing exposure of infants to pollutants in house dust.

PubMed

Roberts, John W; Wallace, Lance A; Camann, David E; Dickey, Philip; Gilbert, Steven G; Lewis, Robert G; Takaro, Tim K

2009-01-01

The health risks to babies from pollutants in house dust may be 100 times greater than for adults. The young ingest more dust and are up to ten times more vulnerable to such exposures. House dust is the main exposure source for infants to allergens, lead, and PBDEs, as well as a major source of exposure to pesticides, PAHs, Gram-negative bacteria, arsenic, cadmium, chromium, phthalates, phenols, and other EDCs, mutagens, and carcinogens. Median or upper percentile concentrations in house dust of lead and several pesticides and PAHs may exceed health-based standards in North America. Early contact with pollutants among the very young is associated with higher rates of chronic illness such as asthma, loss of intelligence, ADHD, and cancer in children and adults. The potential of infants, who live in areas with soil contaminated by automotive and industrial emissions, can be given more protection by improved home cleaning and hand washing. Babies who live in houses built before 1978 have a prospective need for protection against lead exposures; homes built before 1940 have even higher lead exposure risks. The concentration of pollutants in house dust may be 2-32 times higher than that found in the soil near a house. Reducing infant exposures, at this critical time in their development, may reduce lifetime health costs, improve early learning, and increase adult productivity. Some interventions show a very rapid payback. Two large studies provide evidence that home visits to reduce the exposure of children with poorly controlled asthma triggers may return more than 100% on investment in 1 yr in reduced health costs. The tools provided to families during home visits, designed to reduce dust exposures, included vacuum cleaners with dirt finders and HEPA filtration, allergy control bedding covers, high-quality door mats, and HEPA air filters. Infants receive their highest exposure to pollutants in dust at home, where they spend the most time, and where the family has the most mitigation control. Normal vacuum cleaning allows deep dust to build up in carpets where it can be brought to the surface and become airborne as a result of activity on the carpet. Vacuums with dirt finders allow families to use the three-spot test to monitor deep dust, which can reinforce good cleaning habits. Motivated families that receive home visits from trained outreach workers can monitor and reduce dust exposures by 90% or more in 1 wk. The cost of such visits is low considering the reduction of risks achieved. Improved home cleaning is one of the first results observed among families who receive home visits from MHEs and CHWs. We believe that proven intervention methods can reduce the exposure of infants to pollutants in house dust, while recognizing that much remains to be learned about improving the effectiveness of such methods.

Sporting programs for inactive population groups: factors influencing implementation in the organized sports setting.

PubMed

Ooms, Linda; Veenhof, Cindy; Schipper-van Veldhoven, Nicolette; de Bakker, Dinny H

2015-01-01

The organized sports sector has received increased attention as a setting to promote health-enhancing physical activity (HEPA) to the general population. For significant public health impact, it is important that successful HEPA programs are widely adopted, implemented and continued as ongoing practice. The importance of evaluating the context in which programs are implemented has been identified as critical. However, little research has focused on understanding the organized sports implementation context, including factors facilitating and impeding implementation. In this study, the main factors influencing implementation of HEPA programs in the organized sports setting were studied. Fourteen sporting programs in the Netherlands aimed at increasing participation in sports by inactive population groups and funded within the National Action Plan for Sport and Exercise (NAPSE) were investigated. The programs were developed by ten Dutch National Sports Federations (NSFs) and implemented by different sports clubs in the Netherlands over a 3-year implementation period (June 2008-June 2011). The qualitative research component involved yearly face-to-face interviews (i.e. fourteen interviews each year, n = 12 program coordinators) and a group meeting with the program coordinators of the NSFs (n = 8). Cross-case comparisons and thematic analyses were performed to identify and categorize important facilitating and impeding factors respectively. The quantitative research component, used to identify the most important facilitating and impeding factors across all sporting programs, consisted of ranking of factors according to importance by the program coordinators (n = 12). Different factors act during six identified (implementation) phases. When comparing factors across phases, several key learnings were evident. Successful implementation relied, for example, on program design and enthusiastic individuals within sporting organizations. On the other hand, inactive people were hard to reach and participation of sports clubs was not self-evident. The findings were discussed in a broader context. This study adds to the knowledge base concerning the implementation of sporting programs, aimed at inactive people, in the organized sports setting. The main factors facilitating and impeding implementation were identified. The results of this study can be used by sports practitioners and policy makers when developing and implementing HEPA programs in this setting.

Effects of Isoprene- and Toluene-Generated Smog on Allergic ...

EPA Pesticide Factsheets

Reactions of organic compounds with nitric oxide (NO) and sunlight produce complex mixtures of pollutants including secondary organic aerosol (SOA), ozone (O3), nitrogen dioxide (NO2), and reactive aldehydes. The health effects of these photochemical smog mixtures in susceptible populations including asthmatics are unclear. We assessed effects of smog generated from mixtures of NO with isoprene (IS) or toluene (TL) on allergic inflammatory responses in Balb/cJ mice. House dust mite (HDM)-sensitized or control mice were all challenged with HDM intranasally 1 d prior to whole-body inhalation exposure to IS (chamber average 509 ppb NO2, 246 ppb O3, and 160 g/m3 SOA), TL (217 ppb NO2, 129 ppb O3, and 376 g/m3 SOA), or HEPA-filtered air (4 h/d for 2 days). Mice were necropsied within 3 h after the second exposure (2 d post-HDM challenge). Assessment of breathing parameters during exposure with double-chamber plethysmography showed a trend for increased specific airway resistance and decreased minute volume during the second day of TL exposure in both non-allergic and HDM-allergic mice. HDM-allergic air-exposed mice had significant increases in numbers of bronchoalveolar lavage (BAL) alveolar macrophages (AM) and eosinophils (EO), and trends for increases in BAL indices of lung injury in comparison with non-allergic air-exposed mice. Exposure to either IS or TL attenuated the increases in AM, EO, and lung injury markers in HDM-allergic mice. The results of this

Effects of an ethanol-gasoline mixture: results of a 4-week inhalation study in rats.

PubMed

Chu, I; Poon, R; Valli, V; Yagminas, A; Bowers, W J; Seegal, R; Vincent, R

2005-01-01

The inhalation toxicity of an ethanol-gasoline mixture was investigated in rats. Groups of 15 male and 15 female rats were exposed by inhalation to 6130 ppm ethanol, 500 ppm gasoline or a mixture of 85% ethanol and 15% gasoline (by volume, 6130 ppm ethanol and 500 ppm gasoline), 6 h a day, 5 days per week for 4 weeks. Control rats of both genders received HEPA/charcoal-filtered room air. Ten males and ten females from each group were killed after 4 weeks of treatment and the remaining rats were exposed to filtered room air for an additional 4 weeks to determine the reversibility of toxic injuries. Female rats treated with the mixture showed growth suppression, which was reversed after 4 weeks of recovery. Increased kidney weight and elevated liver microsomal ethoxyresorufin-O-deethylase (EROD) activity, urinary ascorbic acid, hippuric acid and blood lymphocytes were observed and most of the effects were associated with gasoline exposure. Combined exposure to ethanol and gasoline appeared to exert an additive effect on growth suppression. Inflammation of the upper respiratory tract was observed only in the ethanol-gasoline mixture groups, and exposure to either ethanol and gasoline had no effect on the organ, suggesting that an irritating effect was produced when the two liquids were mixed. Morphology in the adrenal gland was characterized by vacuolation of the cortical area. Although histological changes were generally mild in male and female rats and were reversed after 4 weeks, the changes tended to be more severe in male rats. Brain biogenic amine levels were altered in ethanol- and gasoline-treated groups; their levels varied with respect to gender and brain region. Although no general interactions were observed in the brain neurotransmitters, gasoline appeared to suppress dopamine concentrations in the nucleus accumbens region co-exposed to ethanol. It was concluded that treatment with ethanol and gasoline, at the levels studied, produced mild, reversible biochemical hematological and histological effects, with some indications of interactions when they were co-administered.

Vaccinations for Preteens and Teens, Age 11-19 Years

MedlinePlus

... Vaccine Do you need it? Chickenpox (varicella; Var) Yes! If you haven’t been vaccinated and haven’ ... should get a second dose. Hepatitis A (HepA) Yes! You need 2 doses of hepatitis A vaccine ...

40 CFR 721.10279 - Multi-walled carbon nanotubes (generic) (P-10-246).

Code of Federal Regulations, 2014 CFR

2014-07-01

... a loose-fitting hood or helmet and high efficiency particulate air (HEPA) filters; or (C) NIOSH... in § 721.80(f), (k) (conductivity additive to resins, rubber, and to battery electrodes), and (q...

Performance Evaluation of Axial Flow AG-1 FC and Prototype FM (High Strength) HEPA Filters - 13123

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giffin, Paxton K.; Parsons, Michael S.; Wilson, John A.

High efficiency particulate air (HEPA) filters are routinely used in DOE nuclear containment activities. The Nuclear Air Cleaning Handbook (NACH) stipulates that air cleaning devices and equipment used in DOE nuclear applications must meet the American Society of Mechanical Engineers (ASME) Code on Nuclear Air and Gas Treatment (AG-1) standard. This testing activity evaluates two different axial flow HEPA filters, those from AG-1 Sections FC and FM. Section FM is under development and has not yet been added to AG-1 due to a lack of qualification data available for these filters. Section FC filters are axial flow units that utilizemore » a fibrous glass filtering medium. The section FM filters utilize a similar fibrous glass medium, but also have scrim backing. The scrim-backed filters have demonstrated the ability to endure pressure impulses capable of completely destroying FC filters. The testing activities presented herein will examine the total lifetime loading for both FC and FM filters under ambient conditions and at elevated conditions of temperature and relative humidity. Results will include loading curves, penetration curves, and testing condition parameters. These testing activities have been developed through collaborations with representatives from the National Nuclear Security Administration (NNSA), DOE Office of Environmental Management (DOE-EM), New Mexico State University, and Mississippi State University. (authors)« less

Preparing cytotoxic agents in an isolator.

PubMed

Favier, M; Hansel, S; Bressolle, F

1993-11-01

The design of an isolator and its use by an oncology satellite pharmacy for preparing cytotoxic drugs are described. The isolator (Iso Concept, Boulogne, France) is a totally enclosed ventilated biological-safety cabinet of class III polyvinyl chloride (PVC) with positive air pressure, a half-suit with a rotating seal, and attached neoprene gloves. There are three work-stations, one for the half-suit and two along one side of the isolator. The ventilation and air filtration system consists of one entry pipe with a full ventilation-filtration box fitted with one prefilter, one blower, one ball valve, one high-efficiency particulate air (HEPA) filter, one airtight nipple connected to an automatic sterilizer, alarms, and one exhaust pipe protected by a HEPA filter. The air lock consists of a rigid, transparent Plexiglas pass-through. The chamber is sterilized with heated compressed air mixed with 3.5% peracetic acid. Maintenance includes regular changing of gloves and HEPA filters; checking of the integrity of the PVC, half-suit, and gloves; and washing and decontamination procedures. Preparation of cytotoxics is planned in advance with prescription data and manufacturing sheets. In the half-suit, a pharmacy technician reads the label, supervises preparation of the sterile admixture, and writes a label. The operators on the side of the unit read the manufacturing sheet and prepare the dose identified by the label.(ABSTRACT TRUNCATED AT 250 WORDS)

Transient Heating and Thermomechanical Stress Modeling of Ceramic HEPA Filters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bogle, Brandon; Kelly, James; Haslam, Jeffrey

The purpose of this report is to showcase an initial finite-element analysis model of a ceramic High-Efficiency Particulate (HEPA) Air filter design. Next generation HEPA filter assemblies are being developed at LLNL to withstand high-temperature fire scenarios by use of ceramics and advanced materials. The filters are meant for use in radiological and nuclear facilities, and are required to survive 500°C fires over an hour duration. During such conditions, however, collecting data under varying parameters can be challenging; therefore, a Finite Element Analysis model of the filter was conducted using COMSOL ® Multiphysics to analyze the effects of fire. Finitemore » Element Analysis (FEA) modelling offers several opportunities: researchers can quickly and easily consider impacts of potential design changes, material selection, and flow characterization on filter performance. Specifically, this model provides stress references for the sealant at high temperatures. Modeling of full filter assemblies was deemed inefficient given the computational requirements, so a section of three tubes from the assembly was modeled. The model looked at the transient heating and thermomechanical stress development during a 500°C air flow at 6 CFM. Significant stresses were found at the ceramic-metal interfaces of the filter, and conservative temperature profiles at locations of interest were plotted. The model can be used for the development of sealants that minimize stresses at the ceramic-metal interface. Further work on the model would include the full filter assembly and consider heat losses to make more accurate predictions.« less

Slurry Coating System Statement of Work and Specification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chan, S. M.

2017-02-06

The Slurry Coating System will be used to coat crystals with a polymer to support Lawrence Livermore National Security, LLC (LLNS) research and development at Lawrence Livermore National Laboratory (LLNL). The crystals will be suspended in water in a kettle. A polymer solution is added, temperature of the kettle is raised and aggregates of the crystals and polymer form. The slurry is heated under vacuum to drive off the solvents and slowly cooled while mixing to room temperature. The resulting aggregates are then filtered and dried. The performance characteristics and fielding constraints define a unique set of requirements for amore » new system. This document presents the specifications and requirements for the system.« less

Comparison between lead levels in dandelions grown in an ultra-clean lab environment (baseline) and those collected from the San Francisco Bay Area

NASA Astrophysics Data System (ADS)

Rojero, J.; Odigie, K. O.; Hibdon, S.; Flegal, A. R.

2011-12-01

This study is aimed at establishing the baseline (natural) levels of lead in dandelions (Taraxacum officinale) grown in an ultra-clean environment. Dandelions have been used extensively as biomonitors of environmental lead levels since their distribution is global and they can be easily collected. However, industrial lead contamination is so pervasive that even dandelions from the most remote areas in the world may be contaminated with industrial lead. Therefore, this work will test the hypothesis that "natural" lead levels in dandelions are lower than any previously published values - by growing them in a HEPA filtered air (Class 100) trace metal clean room with high purity (18 MΩ cm) water. Concentrations and isotopic compositions of lead in the clean-room grown dandelions will be compared to values in literature and to those of lead in dandelions collected from San Francisco Bay Area. Lead is a dense, ductile, and highly malleable metal that is found naturally in our environment. Due to its properties it is currently highly used in building construction, in ceramic glazes, lead chromate and in PVC plastic used to coat electrical cords. The uses of lead have included paint, leather tanning, and being used as an additive to gasoline prior to the mid 1970's, as well as others. Due to its many uses, humans are susceptible to lead regularly through various means of exposure from air, water and soil, often leading to lead toxicity.

77 FR 48967 - Marine Mammals; File No. 17350

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-15

... permit has been issued to the North Slope Borough Department of Wildlife Management, P.O. Box 69, Barrow, AK 99723 [Taqulik Hepa, Responsible Party; Dr. John C. George, Principal Investigator] to collect...

Effect of crystallographic orientation on the anodic formation of nanoscale pores/tubes in TiO 2 films

NASA Astrophysics Data System (ADS)

Kalantar-zadeh, K.; Sadek, A. Z.; Zheng, H.; Partridge, J. G.; McCulloch, D. G.; Li, Y. X.; Yu, X. F.; Wlodarski, W.

2009-10-01

Self-organized nanopores and nanotubes have been produced in thin films of titanium (Ti) prepared using filtered cathodic vacuum arc (FCVA), DC- and RF-sputter deposition systems. The anodization process was performed using a neutral electrolyte containing fluoride ions with an applied potential between 2 and 20 V (for clarity the results are only presented for 5 V). Scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray diffraction (XRD) techniques were used to characterise the films. It was found that the crystallographic orientation of the Ti films played a significant role in determining whether pores or tubes were formed during the anodic etching process.

Development of an approach to correcting MicroPEM baseline drift.

PubMed

Zhang, Ting; Chillrud, Steven N; Pitiranggon, Masha; Ross, James; Ji, Junfeng; Yan, Beizhan

2018-07-01

Fine particulate matter (PM 2.5 ) is associated with various adverse health outcomes. The MicroPEM (RTI, NC), a miniaturized real-time portable particulate sensor with an integrated filter for collecting particles, has been widely used for personal PM 2.5 exposure assessment. Five-day deployments were targeted on a total of 142 deployments (personal or residential) to obtain real-time PM 2.5 levels from children living in New York City and Baltimore. Among these 142 deployments, 79 applied high-efficiency particulate air (HEPA) filters in the field at the beginning and end of each deployment to adjust the zero level of the nephelometer. However, unacceptable baseline drift was observed in a large fraction (> 40%) of acquisitions in this study even after HEPA correction. This drift issue has been observed in several other studies as well. The purpose of the present study is to develop an algorithm to correct the baseline drift in MicroPEM based on central site ambient data during inactive time periods. A running baseline & gravimetric correction (RBGC) method was developed based on the comparison of MicroPEM readings during inactive periods to ambient PM 2.5 levels provided by fixed monitoring sites and the gravimetric weight of PM 2.5 collected on the MicroPEM filters. The results after RBGC correction were compared with those using HEPA approach and gravimetric correction alone. Seven pairs of duplicate acquisitions were used to validate the RBGC method. The percentages of acquisitions with baseline drift problems were 42%, 53% and 10% for raw, HEPA corrected, and RBGC corrected data, respectively. Pearson correlation analysis of duplicates showed an increase in the coefficient of determination from 0.75 for raw data to 0.97 after RBGC correction. In addition, the slope of the regression line increased from 0.60 for raw data to 1.00 after RBGC correction. The RBGC approach corrected the baseline drift issue associated with MicroPEM data. The algorithm developed has the potential for use with data generated from other types of PM sensors that contain a filter for weighing as well. In addition, this approach can be applied in many other regions, given widely available ambient PM data from monitoring networks, especially in urban areas. Copyright © 2018 Elsevier Inc. All rights reserved.

The effectiveness of stand alone air cleaners for shelter-in-place.

PubMed

Ward, M; Siegel, J A; Corsi, R L

2005-04-01

Stand-alone air cleaners may be efficient for rapid removal of indoor fine particles and have potential use for shelter-in-place (SIP) strategies following acts of bioterrorism. A screening model was employed to ascertain the potential significance of size-resolved particle (0.1-2 microm) removal using portable high efficiency particle arresting (HEPA) air cleaners in residential buildings following an outdoor release of particles. The number of stand-alone air cleaners, air exchange rate, volumetric flow rate through the heating, ventilating and air-conditioning (HVAC) system, and size-resolved particle removal efficiency in the HVAC filter were varied. The effectiveness of air cleaners for SIP was evaluated in terms of the outdoor and the indoor particle concentration with air cleaner(s) relative to the indoor concentration without air cleaners. Through transient and steady-state analysis of the model it was determined that one to three portable HEPA air cleaners can be effective for SIP following outdoor bioaerosol releases, with maximum reductions in particle concentrations as high as 90% relative to conditions in which an air cleaner is not employed. The relative effectiveness of HEPA air cleaners vs. other removal mechanisms was predicted to decrease with increasing particle size, because of increasing competition by particle deposition with indoor surfaces and removal to HVAC filters. However, the effect of particle size was relatively small for most scenarios considered here. The results of a screening analysis suggest that stand-alone (portable) air cleaners that contain high efficiency particle arresting (HEPA) filters can be effective for reducing indoor fine particle concentrations in residential dwellings during outdoor releases of biological warfare agents. The relative effectiveness of stand-alone air cleaners for reducing occupants' exposure to particles of outdoor origin depends on several factors, including the type of heating, ventilating and air-conditioning (HVAC) filter, HVAC operation, building air exchange rate, particle size, and duration of elevated outdoor particle concentration. Maximum particle reductions, relative to no stand-alone air cleaners, of 90% are predicted when three stand-alone air cleaners are employed.

Isolation and preliminary characterization of aerobic heterotrophic bacteria isolated from sub-glacial Antarctic water samples

NASA Astrophysics Data System (ADS)

Palma-Alvarez, R.; Lanoil, B. D.

2002-05-01

Recently, evidence has been accumulating supporting the presence of biogeochemically active microbial communities in cold, dark, and isolated subglacial environments. These environments are important sites of rock weathering, provide insight into global biogeochemistry during glacial periods, and are potential analogues for ancient Snowball Earth events and the ice-covered oceans of the Jovian moon, Europa. However, the extent of microbial influence on subglacial geochemistry is unclear. As part of an ongoing project to address the extent of that influence, we isolated aerobic heterotrophic bacteria from sediment-laden water from beneath Ice Stream C, a fast moving region of the Western Antarctic Ice Sheet (WAIS). Plates of a standard environmental media (R2A) were prepared at three dilutions (1x, 0.1x, 0.01x) and inoculated in duplicate in a HEPA-filtered environment. One replicate was incubated at 4oC, the other at room temperature in the dark. All plates showed abundant growth, although colony size was positively correlated with media concentration. One-hundred eighty-one colonies total were picked, grown in liquid R2A (1x concentration) at the same initial temperature, and characterized for Gram character, cell shape, cell size, and production of a diffusible yellow pigment with similar chemical characteristics to the siderophore, pyoverdine. Based on these characters, a moderate level of diversity was observed in these isolates. A few types dominated the samples, with several others found only rarely. Further characterization of these isolates is ongoing, and results of these studies and their possible implications for sub-glacial biogeochemistry are discussed.

40 CFR 63.542 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... to monitor relative particulate matter loadings. Battery breaking area means the plant location at which lead-acid batteries are broken, crushed, or disassembled and separated into components. Blast...) Casting operations occur. High efficiency particulate air (HEPA) filter means a filter that has been...

Sintered composite filter

DOEpatents

Bergman, W.

1986-05-02

A particulate filter medium formed of a sintered composite of 0.5 micron diameter quartz fibers and 2 micron diameter stainless steel fibers is described. Preferred composition is about 40 vol.% quartz and about 60 vol.% stainless steel fibers. The media is sintered at about 1100/sup 0/C to bond the stainless steel fibers into a cage network which holds the quartz fibers. High filter efficiency and low flow resistance are provided by the smaller quartz fibers. High strength is provided by the stainless steel fibers. The resulting media has a high efficiency and low pressure drop similar to the standard HEPA media, with tensile strength at least four times greater, and a maximum operating temperature of about 550/sup 0/C. The invention also includes methods to form the composite media and a HEPA filter utilizing the composite media. The filter media can be used to filter particles in both liquids and gases.

Industrial-hygiene survey report, Wabash Magnetic Company, Huntington, Indiana, May 24-26, 1988. [4,4'-methylenedianiline

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boeniger, M.F.

1989-01-01

A walk-through survey was made at Wabash Magnetic Company, located in Huntington, Indiana for the purpose of determining the extent of worker exposure to 4,4'-methylenedianiline (MDA) in a batch type mixing operation and user of B-stage resins. At this site the chemical was used as a hardening agent to produce the resins used to make transfer-molded parts. Air, wipe, bulk, and urine samples were taken to determine exposure levels. In both the formulating and molding departments, evidence was found for the possible inhalation of and dermal contact with MDA. Air concentrations were detectable, but were below the permissible exposure levelmore » of 10 parts per billion (ppb) proposed by OSHA. Of seven workers monitored by urinalysis, two were excreting detectable amounts of MDA with the higher concentration being 16 ppb. The author recommends a combination of modified work practices and alternative personal protection to reduce exposures. A vacuum installed with a HEPA filter should be used in the dry cleanup processes in the mixing and formulating departments and workers should wear a glove more resistant to permeation than the cotton ones now in use at the site. Gloves made of polyvinylchloride or natural latex are suggested.« less

Vaccinations for Adults with Hepatitis C Infection

MedlinePlus

... up to date. Vaccine Do you need it? Hepatitis A (HepA) Yes! Your chronic liver disease puts you ... serious complications if you get infected with the hepatitis A virus. If you’ve never been vaccinated against ...

A Pollen Primer | NIH MedlinePlus the Magazine

MedlinePlus

... air filters (HEPA) or an electrostatic air filter. Tree Pollen Trees produce pollen earliest, as soon as January in ... distributed miles away. Fewer than 100 kinds of trees cause allergies. Some common ones are catalpa, elm, ...

One Health approach to controlling a Q fever outbreak on an Australian goat farm.

PubMed

Bond, K A; Vincent, G; Wilks, C R; Franklin, L; Sutton, B; Stenos, J; Cowan, R; Lim, K; Athan, E; Harris, O; Macfarlane-Berry, L; Segal, Y; Firestone, S M

2016-04-01

A recent outbreak of Q fever was linked to an intensive goat and sheep dairy farm in Victoria, Australia, 2012-2014. Seventeen employees and one family member were confirmed with Q fever over a 28-month period, including two culture-positive cases. The outbreak investigation and management involved a One Health approach with representation from human, animal, environmental and public health. Seroprevalence in non-pregnant milking goats was 15% [95% confidence interval (CI) 7-27]; active infection was confirmed by positive quantitative PCR on several animal specimens. Genotyping of Coxiella burnetii DNA obtained from goat and human specimens was identical by two typing methods. A number of farming practices probably contributed to the outbreak, with similar precipitating factors to the Netherlands outbreak, 2007-2012. Compared to workers in a high-efficiency particulate arrestance (HEPA) filtered factory, administrative staff in an unfiltered adjoining office and those regularly handling goats and kids had 5·49 (95% CI 1·29-23·4) and 5·65 (95% CI 1·09-29·3) times the risk of infection, respectively; suggesting factory workers were protected from windborne spread of organisms. Reduction in the incidence of human cases was achieved through an intensive human vaccination programme plus environmental and biosecurity interventions. Subsequent non-occupational acquisition of Q fever in the spouse of an employee, indicates that infection remains endemic in the goat herd, and remains a challenge to manage without source control.

Factors affecting ozone removal rates in a simulated aircraft cabin environment

NASA Astrophysics Data System (ADS)

Tamás, Gyöngyi; Weschler, Charles J.; Bakó-Biró, Zsolt; Wyon, David P.; Strøm-Tejsen, Peter

Ozone concentrations were measured concurrently inside a simulated aircraft cabin and in the airstream providing ventilation air to the cabin. Ozone decay rates were also measured after cessation of ozone injection into the supply airstream. By systematically varying the presence or absence of people, soiled T-shirts, aircraft seats and a used HEPA filter, we have been able in the course of 24 experiments to isolate the contributions of these and other factors to the removal of ozone from the cabin air. In the case of this simulated aircraft, people were responsible for almost 60% of the ozone removal occurring within the cabin and recirculation system; respiration can only have been responsible for about 4% of this removal. The aircraft seats removed about 25% of the ozone; the loaded HEPA filter, 7%; and the other surfaces, 10%. A T-shirt that had been slept in overnight removed roughly 70% as much ozone as a person, indicating the importance of skin oils in ozone removal. The presence of the used HEPA filter in the recirculated airstream reduced the perceived air quality. Over a 5-h period, the overall ozone removal rate by cabin surfaces decreased at ˜3% h -1. With people present, the measured ratio of ozone's concentration in the cabin versus that outside the cabin was 0.15-0.21, smaller than levels reported in the literature. The results reinforce the conclusion that the optimal way to reduce people's exposure to both ozone and ozone oxidation products is to efficiently remove ozone from the air supply system of an aircraft.

The effect of high-efficiency particulate air respirator design on occupational health: a pilot study balancing risks in the real world.

PubMed

Eck, E K; Vannier, A

1997-02-01

To quantify specific factors believed to increase healthcare worker (HCW) risk for contaminated sharps injuries (eg, visibility, communication, and range of motion); to quantify the degree to which respirators of various designs impacted those same factors; and to assess HCW opinions about the suitability of selected respirators with respect to patient care and user compliance criteria. Sharps injury data from seven hospitals were analyzed to determine the potential contribution of visibility, communication, and range of motion to reported injuries. Healthcare workers representing various clinical specialties and physical characteristics were examined at baseline and while wearing five different respirators to quantify the impact of respirator design on visibility, communication, and range of motion. Healthcare workers were interviewed and completed a survey assessing each respirator. Hospital and ambulatory-care settings. Population-based and convenience sample. Communication, visibility, and range of motion were found to affect contaminated sharps injuries significantly. Selected high-efficiency particulate air (HEPA) respirators were found to have a negative impact on each of these variables. Healthcare workers involved in the study also reported compliance criteria problems with selected HEPA respirators, which may effect implementation of respiratory precautions adversely. Current HEPA respirators, because of their design, potentially increase the risk of bloodborne pathogen exposure through sharps injuries. We conclude that mandating respirators without regard to the potential impact of their design to the sharps injuries may be counterproductive to HCW safety, because they may increase, rather than decrease, overall occupational risk to HCWs.

Detection and Removal of Impurities in Nitric Oxide Generated from Air by Pulsed Electrical Discharge

PubMed Central

Yu, Binglan; Blaesi, Aron H.; Casey, Noel; Raykhtsaum, Grigory; Zazzeron, Luca; Jones, Rosemary; Morrese, Alexander; Dobrynin, Danil; Malhotra, Rajeev; Bloch, Donald B.; Goldstein, Lee E.; Zapol, Warren M.

2016-01-01

Inhalation of nitric oxide (NO) produces selective pulmonary vasodilation without dilating the systemic circulation. However, the current NO/N2 cylinder delivery system is cumbersome and expensive. We developed a lightweight, portable, and economical device to generate NO from air by pulsed electrical discharge. The objective of this study was to investigate and optimize the purity and safety of NO generated by this device. By using low temperature streamer discharges in the plasma generator, we produced therapeutic levels of NO with very low levels of nitrogen dioxide (NO2) and ozone. Despite the low temperature, spark generation eroded the surface of the electrodes, contaminating the gas stream with metal particles. During prolonged NO generation there was gradual loss of the iridium high-voltage tip (−90 µg/day) and the platinum-nickel ground electrode (−55 µg/day). Metal particles released from the electrodes were trapped by a high-efficiency particulate air (HEPA) filter. Quadrupole mass spectroscopy measurements of effluent gas during plasma NO generation showed that a single HEPA filter removed all of the metal particles. Mice were exposed to breathing 50 parts per million of electrically generated NO in air for 28 days with only a scavenger and no HEPA filter; the mice did not develop pulmonary inflammation or structural changes and iridium and platinum particles were not detected in the lungs of these mice. In conclusion, an electric plasma generator produced therapeutic levels of NO from air; scavenging and filtration effectively eliminated metallic impurities from the effluent gas. PMID:27592386

Detection and removal of impurities in nitric oxide generated from air by pulsed electrical discharge.

PubMed

Yu, Binglan; Blaesi, Aron H; Casey, Noel; Raykhtsaum, Grigory; Zazzeron, Luca; Jones, Rosemary; Morrese, Alexander; Dobrynin, Danil; Malhotra, Rajeev; Bloch, Donald B; Goldstein, Lee E; Zapol, Warren M

2016-11-30

Inhalation of nitric oxide (NO) produces selective pulmonary vasodilation without dilating the systemic circulation. However, the current NO/N 2 cylinder delivery system is cumbersome and expensive. We developed a lightweight, portable, and economical device to generate NO from air by pulsed electrical discharge. The objective of this study was to investigate and optimize the purity and safety of NO generated by this device. By using low temperature streamer discharges in the plasma generator, we produced therapeutic levels of NO with very low levels of nitrogen dioxide (NO 2 ) and ozone. Despite the low temperature, spark generation eroded the surface of the electrodes, contaminating the gas stream with metal particles. During prolonged NO generation there was gradual loss of the iridium high-voltage tip (-90 μg/day) and the platinum-nickel ground electrode (-55 μg/day). Metal particles released from the electrodes were trapped by a high-efficiency particulate air (HEPA) filter. Quadrupole mass spectroscopy measurements of effluent gas during plasma NO generation showed that a single HEPA filter removed all of the metal particles. Mice were exposed to breathing 50 parts per million of electrically generated NO in air for 28 days with only a scavenger and no HEPA filter; the mice did not develop pulmonary inflammation or structural changes and iridium and platinum particles were not detected in the lungs of these mice. In conclusion, an electric plasma generator produced therapeutic levels of NO from air; scavenging and filtration effectively eliminated metallic impurities from the effluent gas. Copyright © 2016 Elsevier Inc. All rights reserved.

40 CFR 63.11423 - What are the standards and compliance requirements for new and existing sources?

Code of Federal Regulations, 2011 CFR

2011-07-01

... Lead Acid Battery Manufacturing Area Sources Standards and Compliance Requirements § 63.11423 What are.... Fabric filters equipped with a high efficiency particulate air (HEPA) filter or other secondary filter...

40 CFR 63.11423 - What are the standards and compliance requirements for new and existing sources?

Code of Federal Regulations, 2012 CFR

2012-07-01

... Lead Acid Battery Manufacturing Area Sources Standards and Compliance Requirements § 63.11423 What are.... Fabric filters equipped with a high efficiency particulate air (HEPA) filter or other secondary filter...

40 CFR 63.11423 - What are the standards and compliance requirements for new and existing sources?

Code of Federal Regulations, 2013 CFR

2013-07-01

... Lead Acid Battery Manufacturing Area Sources Standards and Compliance Requirements § 63.11423 What are.... Fabric filters equipped with a high efficiency particulate air (HEPA) filter or other secondary filter...

40 CFR 63.11423 - What are the standards and compliance requirements for new and existing sources?

Code of Federal Regulations, 2014 CFR

2014-07-01

... Lead Acid Battery Manufacturing Area Sources Standards and Compliance Requirements § 63.11423 What are.... Fabric filters equipped with a high efficiency particulate air (HEPA) filter or other secondary filter...

40 CFR 63.11423 - What are the standards and compliance requirements for new and existing sources?

Code of Federal Regulations, 2010 CFR

2010-07-01

... Lead Acid Battery Manufacturing Area Sources Standards and Compliance Requirements § 63.11423 What are.... Fabric filters equipped with a high efficiency particulate air (HEPA) filter or other secondary filter...

Hydrogenation behavior of Ti-implanted Zr-1Nb alloy with TiN films deposited using filtered vacuum arc and magnetron sputtering

NASA Astrophysics Data System (ADS)

Kashkarov, E. B.; Nikitenkov, N. N.; Sutygina, A. N.; Bezmaternykh, A. O.; Kudiiarov, V. N.; Syrtanov, M. S.; Pryamushko, T. S.

2018-02-01

More than 60 years of operation of water-cooled reactors have shown that local or general critical hydrogen concentration is one of the basic limiting criteria of zirconium-based fuel element claddings. During the coolant radiolysis, released hydrogen penetrates and accumulates in zirconium alloys. Hydrogenation of zirconium alloys leads to degradation of their mechanical properties, hydride cracking and stress corrosion cracking. In this research the effect of titanium nitride (TiN) deposition on hydrogenation behavior of Ti-implanted Zr-1Nb alloy was described. Ti-implanted interlayer was fabricated by plasma immersion ion implantation (PIII) at the pulsed bias voltage of 1500 V to improve the adhesion of TiN and reduce hydrogen penetration into Zr-1Nb alloy. We conducted the comparative analysis on hydrogenation behavior of the Ti-implanted alloy with sputtered and evaporated TiN films by reactive dc magnetron sputtering (dcMS) and filtered cathodic vacuum arc deposition (FVAD), respectively. The crystalline structure and surface morphology were investigated using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The elemental distribution was analyzed using glow-discharge optical emission spectroscopy (GD-OES). Hydrogenation was performed from gas atmosphere at 350 °C and 2 atm hydrogen pressure. The results revealed that TiN films as well as Ti implantation significantly reduce hydrogen absorption rate of Zr-1Nb alloy. The best performance to reduce the rate of hydrogen absorption is Ti-implanted layer with evaporated TiN film. Morphology of the films impacted hydrogen permeation through TiN films: the denser film the lower hydrogen permeation. The Ti-implanted interface plays an important role of hydrogen accumulation layer for trapping the penetrated hydrogen. No deterioration of adhesive properties of TiN films on Zr-1Nb alloy with Ti-implanted interface occurs under high-temperature hydrogen exposure. Thus, the fabrication of Ti-implanted layer with dense TiN films can be an effective way to protect Zr-1Nb alloy from hydrogen embrittlement.

40 CFR 63.542 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... to monitor relative particulate matter loadings. Battery breaking area means the plant location at which lead-acid batteries are broken, crushed, or disassembled and separated into components. Blast...) Casting operations occur. High efficiency particulate air (HEPA) filter means a filter that has been...

Amorphous-diamond electron emitter

DOEpatents

Falabella, Steven

2001-01-01

An electron emitter comprising a textured silicon wafer overcoated with a thin (200 .ANG.) layer of nitrogen-doped, amorphous-diamond (a:D-N), which lowers the field below 20 volts/micrometer have been demonstrated using this emitter compared to uncoated or diamond coated emitters wherein the emission is at fields of nearly 60 volts/micrometer. The silicon/nitrogen-doped, amorphous-diamond (Si/a:D-N) emitter may be produced by overcoating a textured silicon wafer with amorphous-diamond (a:D) in a nitrogen atmosphere using a filtered cathodic-arc system. The enhanced performance of the Si/a:D-N emitter lowers the voltages required to the point where field-emission displays are practical. Thus, this emitter can be used, for example, in flat-panel emission displays (FEDs), and cold-cathode vacuum electronics.

Sintered composite medium and filter

DOEpatents

Bergman, Werner

1987-01-01

A particulate filter medium is formed of a sintered composite of 0.5 micron diameter quartz fibers and 2 micron diameter stainless steel fibers. A preferred composition is about 40 vol. % quartz and about 60 vol. % stainless steel fibers. The media is sintered at about 1100.degree. C. to bond the stainless steel fibers into a cage network which holds the quartz fibers. High filter efficiency and low flow resistance are provided by the smaller quartz fibers. High strength is provided by the stainless steel fibers. The resulting media has a high efficiency and low pressure drop similar to the standard HEPA media, with tensile strength at least four times greater, and a maximum operating temperature of about 550.degree. C. The invention also includes methods to form the composite media and a HEPA filter utilizing the composite media. The filter media can be used to filter particles in both liquids and gases.

Rapid and automated enumeration of viable bacteria in compost using a micro-colony auto counting system.

PubMed

Wang, Xiaodan; Yamaguchi, Nobuyasu; Someya, Takashi; Nasu, Masao

2007-10-01

The micro-colony method was used to enumerate viable bacteria in composts. Cells were vacuum-filtered onto polycarbonate filters and incubated for 18 h on LB medium at 37 degrees C. Bacteria on the filters were stained with SYBR Green II, and enumerated using a newly developed micro-colony auto counting system which can automatically count micro-colonies on half the area of the filter within 90 s. A large number of bacteria in samples retained physiological activity and formed micro-colonies within 18 h, whereas most could not form large colonies on conventional media within 1 week. The results showed that this convenient technique can enumerate viable bacteria in compost rapidly for its efficient quality control.

Edge-emitting ultraviolet n-ZnO:Al/i-ZnO/p-GaN heterojunction light-emitting diode with a rib waveguide.

PubMed

Liang, H K; Yu, S F; Yang, H Y

2010-02-15

An edge-emitting ultraviolet n-ZnO:Al/i-ZnO/p-GaN heterojunction light-emitting diode with a rib waveguide is fabricated by filtered cathodic vacuum arc technique at low deposition temperature (approximately 150 degrees C). Electroluminescence with emission peak at 387 nm is observed. Good correlation between electro- and photo- luminescence spectra suggests that the i-ZnO layer of the heterojunction supports radiative excitonic recombination. Furthermore, it is found that the emission intensity can be enhanced by approximately 5 times due to the presence of the rib waveguide. Only fundamental TE and TM polarizations are supported inside the rib waveguide and the intensity of TE polarization is approximately 2.2 time larger than that of TM polarization.

29 CFR 1926.1127 - Cadmium.

Code of Federal Regulations, 2012 CFR

2012-07-01

... from that process. High-efficiency Particulate Air [HEPA] filter means a filter capable of trapping and...) expected to have the highest cadmium exposures. (2) Specific. (i) Initial monitoring. Except as provided... 1926.354, where applicable. (3) Prohibitions. (i) High speed abrasive disc saws and similar abrasive...

29 CFR 1926.1127 - Cadmium.

Code of Federal Regulations, 2014 CFR

2014-07-01

... from that process. High-efficiency Particulate Air [HEPA] filter means a filter capable of trapping and...) expected to have the highest cadmium exposures. (2) Specific. (i) Initial monitoring. Except as provided... 1926.354, where applicable. (3) Prohibitions. (i) High speed abrasive disc saws and similar abrasive...

29 CFR 1926.1127 - Cadmium.

Code of Federal Regulations, 2010 CFR

2010-07-01

... from that process. High-efficiency Particulate Air [HEPA] filter means a filter capable of trapping and...) expected to have the highest cadmium exposures. (2) Specific. (i) Initial monitoring. Except as provided... 1926.354, where applicable. (3) Prohibitions. (i) High speed abrasive disc saws and similar abrasive...

29 CFR 1926.1127 - Cadmium.

Code of Federal Regulations, 2013 CFR

2013-07-01

... from that process. High-efficiency Particulate Air [HEPA] filter means a filter capable of trapping and...) expected to have the highest cadmium exposures. (2) Specific. (i) Initial monitoring. Except as provided... 1926.354, where applicable. (3) Prohibitions. (i) High speed abrasive disc saws and similar abrasive...

Engineering study for a melting, casting, rolling and fabrication facility for recycled contaminated stainless steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

This Preliminary Report is prepared to study the facilities required for recycling contaminated stainless steel scrap into plate which will be fabricated into boxes suitable for the storage of contaminated wastes and rubble. The study is based upon the underlying premise that the most cost effective way to produce stainless steel is to use the same processes employed by companies now in production of high quality stainless steel. Therefore, the method selected for this study for the production of stainless steel plate from scrap is conventional process using an Electric Arc Furnace for meltdown to hot metal, a Continuous Castermore » for production of cast slabs, and a Reversing Hot Mill for rolling the slabs into plate. The fabrication of boxes from the plate utilizes standard Shears, Punch Presses and welding equipment with Robotic Manipulators. This Study presumes that all process fumes, building dusts and vapors will be cycled through a baghouse and a nuclear grade HEPA filter facility prior to discharge. Also, all process waste water will be evaporated into the hot flue gas stream from the furnace utilizing a quench tank; so there will be no liquid discharges from the facility and all vapors will be processed through a HEPA filter. Even though HEPA filters are used today in controlling radioactive contamination from nuclear facilities there is a sparsity of data concerning radioactivity levels and composition of waste that may be collected from contaminated scrap steel processing. This report suggests some solutions to these problems but it is recommended that additional study must be given to these environmental problems.« less

Mice exposed in situ to urban air pollution exhibit pulmonary alterations in gene expression in the lipid droplet synthesis pathways.

PubMed

Rowan-Carroll, Andrea; Halappanavar, Sabina; Williams, Andrew; Somers, Christophers M; Yauk, Carole L

2013-05-01

It is clear that particulate air pollution poses a serious risk to human health; however, the underlying mechanisms are not completely understood. We investigated pulmonary transcriptional responses in mice following in-situ exposure to ambient air in a heavily industrialized urban environment. Mature C57BL/CBA male mice were caged in sheds near two working steel mills and a major highway in Hamilton, Ontario, Canada in the spring/summer of 2004. Control mice were housed in the same environment, but received only high-efficiency particle filtered air (HEPA). Whole lung tissues were collected from mice exposed for 3, 10, or for 10 weeks followed by 6 weeks recovery in the laboratory (16 weeks). DNA microarrays were used to profile changes in pulmonary gene expression. Transcriptional profiling revealed changes in the expression of genes implicated in the lipid droplet synthesis (Plin I, Dgat2, Lpl, S3-12, and Agpat2), and antioxidant defense (Ucp1) pathways in mice breathing unfiltered air. We postulate that exposure to urban air, containing an abundance of particulate matter adsorbed with polycyclic aromatic hydrocarbons, triggers lipid droplet (holding depots for lipids and malformed/excess proteins tagged for degradation) synthesis in the lungs, which may act to sequester particulates. Increased lipid droplet synthesis could lead to endogenous/stressor-induced production of reactive oxygen species and activation of antioxidant mechanisms. Further investigation into the stimulation of lipid droplet synthesis in the lung in response to air pollution and the resulting health implications is warranted. Copyright © 2013 Wiley Periodicals, Inc.

Making healthy eating and physical activity policy practice: the design and overview of a group randomized controlled trial in afterschool programs.

PubMed

Beets, Michael W; Glenn Weaver, R; Turner-McGrievy, Gabrielle; Huberty, Jennifer; Ward, Dianne S; Freedman, Darcy A; Saunders, Ruth; Pate, Russell R; Beighle, Aaron; Hutto, Brent; Moore, Justin B

2014-07-01

National and state organizations have developed policies calling upon afterschool programs (ASPs, 3-6 pm) to serve a fruit or vegetable (FV) each day for snack, while eliminating foods and beverages high in added-sugars, and to ensure children accumulate a minimum of 30 min/d of moderate-to-vigorous physical activity (MVPA). Few efficacious and cost-effective strategies exist to assist ASP providers in achieving these important public health goals. This paper reports on the design and conceptual framework of Making Healthy Eating and Physical Activity (HEPA) Policy Practice in ASPs, a 3-year group randomized controlled trial testing the effectiveness of strategies designed to improve snacks served and increase MVPA in children attending community-based ASPs. Twenty ASPs, serving over 1800 children (6-12 years) will be enrolled and match-paired based on enrollment size, average daily min/d MVPA, and days/week FV served, with ASPs randomized after baseline data collection to immediate intervention or a 1-year delayed group. The framework employed, STEPs (Strategies To Enhance Practice), focuses on intentional programming of HEPA in each ASPs' daily schedule, and includes a grocery store partnership to reduce price barriers to purchasing FV, professional development training to promote physical activity to develop core physical activity competencies, as well as ongoing technical support/assistance. Primary outcome measures include children's accelerometry-derived MVPA and time spend sedentary while attending an ASP, direct observation of staff HEPA promoting and inhibiting behaviors, types of snacks served, and child consumption of snacks, as well as, cost of snacks via receipts and detailed accounting of intervention delivery costs to estimate cost-effectiveness. Copyright © 2014 Elsevier Inc. All rights reserved.

High-efficiency particulate air filter test stand and aerosol generator for particle loading studies

NASA Astrophysics Data System (ADS)

Arunkumar, R.; Hogancamp, Kristina U.; Parsons, Michael S.; Rogers, Donna M.; Norton, Olin P.; Nagel, Brian A.; Alderman, Steven L.; Waggoner, Charles A.

2007-08-01

This manuscript describes the design, characterization, and operational range of a test stand and high-output aerosol generator developed to evaluate the performance of 30×30×29cm3 nuclear grade high-efficiency particulate air (HEPA) filters under variable, highly controlled conditions. The test stand system is operable at volumetric flow rates ranging from 1.5to12standardm3/min. Relative humidity levels are controllable from 5%-90% and the temperature of the aerosol stream is variable from ambient to 150°C. Test aerosols are produced through spray drying source material solutions that are introduced into a heated stainless steel evaporation chamber through an air-atomizing nozzle. Regulation of the particle size distribution of the aerosol challenge is achieved by varying source solution concentrations and through the use of a postgeneration cyclone. The aerosol generation system is unique in that it facilitates the testing of standard HEPA filters at and beyond rated media velocities by consistently providing, into a nominal flow of 7standardm3/min, high mass concentrations (˜25mg/m3) of dry aerosol streams having count mean diameters centered near the most penetrating particle size for HEPA filters (120-160nm). Aerosol streams that have been generated and characterized include those derived from various concentrations of KCl, NaCl, and sucrose solutions. Additionally, a water insoluble aerosol stream in which the solid component is predominantly iron (III) has been produced. Multiple ports are available on the test stand for making simultaneous aerosol measurements upstream and downstream of the test filter. Types of filter performance related studies that can be performed using this test stand system include filter lifetime studies, filtering efficiency testing, media velocity testing, evaluations under high mass loading and high humidity conditions, and determination of the downstream particle size distributions.

10 CFR 850.3 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

..., that has end-use functions that depend in whole or in part on its shape or design during end use, and... Element delegates his or her functions under this part. High-efficiency particulate air (HEPA) filter... in this part: Action level means the level of airborne concentration of beryllium established...

Adaptation to HIF-1 deficiency by upregulation of the AMP/ATP ratio and phosphofructokinase activation in hepatomas.

PubMed

Golinska, Monika; Troy, Helen; Chung, Yuen-Li; McSheehy, Paul M; Mayr, Manuel; Yin, Xiaoke; Ly, Lucy; Williams, Kaye J; Airley, Rachel E; Harris, Adrian L; Latigo, John; Perumal, Meg; Aboagye, Eric O; Perrett, David; Stubbs, Marion; Griffiths, John R

2011-05-25

HIF-1 deficiency has marked effects on tumour glycolysis and growth. We therefore investigated the consequences of HIF-1 deficiency in mice, using the well established Hepa-1 wild-type (WT) and HIF-1β-deficient (c4) model. These mechanisms could be clinically relevant, since HIF-1 is now a therapeutic target. Hepa-1 WT and c4 tumours grown in vivo were analysed by 18FDG-PET and 19FDG Magnetic Resonance Spectroscopy for glucose uptake; by HPLC for adenine nucleotides; by immunohistochemistry for GLUTs; by immunoblotting and by DIGE followed by tandem mass spectrometry for protein expression; and by classical enzymatic methods for enzyme activity. HIF-1β deficient Hepa-1 c4 tumours grew significantly more slowly than WT tumours, and (as expected) showed significantly lower expression of many glycolytic enzymes. However, HIF-1β deficiency caused no significant change in the rate of glucose uptake in c4 tumours compared to WT when assessed in vivo by measuring fluoro-deoxyglucose (FDG) uptake. Immunohistochemistry demonstrated less GLUT-1 in c4 tumours, whereas GLUT-2 (liver type) was similar to WT. Factors that might upregulate glucose uptake independently of HIF-1 (phospho-Akt, c-Myc) were shown to have either lower or similar expression in c4 compared to WT tumours. However the AMP/ATP ratio was 4.5 fold higher (p < 0.01) in c4 tumours, and phosphofructokinase-1 (PFK-1) activity, measured at prevailing cellular ATP and AMP concentrations, was up to two-fold higher in homogenates of the deficient c4 cells and tumours compared to WT (p < 0.001), suggesting that allosteric PFK activation could explain their normal level of glycolysis. Phospho AMP-Kinase was also higher in the c4 tumours. Despite their defective HIF-1 and consequent down-regulation of glycolytic enzyme expression, Hepa-1 c4 tumours maintain glucose uptake and glycolysis because the resulting low [ATP] high [AMP] allosterically activate PFK-1. This mechanism of resistance would keep glycolysis functioning and also result in activation of AMP-Kinase and growth inhibition; it may have major implications for the therapeutic activity of HIF inhibitors in vivo. Interestingly, this control mechanism does not involve transcriptional control or proteomics, but rather the classical activation and inhibition mechanisms of glycolytic enzymes.

Aspergillus fumigatus viability drives allergic responses to inhaled conidia.

PubMed

Nayak, Ajay P; Croston, Tara L; Lemons, Angela R; Goldsmith, W T; Marshall, Nikki B; Kashon, Michael L; Germolec, Dori R; Beezhold, Donald H; Green, Brett J

2018-04-13

Aspergillus fumigatus induced allergic airway disease has been shown to involve conidial germination in vivo but the immunological mechanisms remain uncharacterized. A subchronic murine exposure model was used to examine the immunological mediators that are regulated in response to either culturable or non-culturable A. fumigatus conidia. Female B6C3F1/N mice were repeatedly dosed via inhalation with 1 x 105 viable or heat inactivated conidia (HIC), twice a week for 13 weeks (26 exposures). Control mice inhaled HEPA-filtered air. The influence of A. fumigatus conidial germination on the pulmonary immunopathological outcomes was evaluated by flow cytometry analysis of cellular infiltration in the airways, assessment of lung mRNA expression, and quantitative proteomics and histopathology of whole lung tissue. Repeated inhalation of viable conidia, but not HIC, resulted in allergic inflammation marked by vascular remodeling, extensive eosinophilia, and accumulation of alternatively activated macrophages (AAMs) in the murine airways. More specifically, mice that inhaled viable conidia resulted in a mixed TH1 and TH2 (IL-13) cytokine response. Recruitment of eosinophils corresponded with increased Ccl11 transcripts. Furthermore, genes associated with M2 or alternatively activated macrophage polarization (e.g. Arg1, Chil3 and Retnla) were significantly upregulated in viable A. fumigatus exposed mice. In mice inhaling HIC, CD4+ T cells expressing IFN-γ (TH1) dominated the lymphocytic infiltration. Quantitative proteomics of the lung revealed metabolic reprogramming accompanied by mitochondrial dysfunction and endoplasmic reticulum stress stimulated by oxidative stress from repetitive microbial insult. Our studies demonstrate that A. fumigatus conidial viability in vivo is critical to the immunopathological presentation of chronic fungal allergic disease. Copyright © 2018. Published by Elsevier Inc.

Comparative cardiopulmonary effects of size-fractionated airborne particulate matter.

PubMed

Amatullah, Hajera; North, Michelle L; Akhtar, Umme S; Rastogi, Neeraj; Urch, Bruce; Silverman, Frances S; Chow, Chung-Wai; Evans, Greg J; Scott, Jeremy A

2012-02-01

Strong epidemiological evidence exists linking particulate matter (PM) exposures with hospital admissions of individuals for cardiopulmonary symptoms. The PM size is important in influencing the extent of infiltration into the respiratory tract and systemic circulation and directs the differential physiological impacts. To investigate the differential effects of the quasi-ultrafine (PM(0.2)), fine (PM(0.15-2.5)), and coarse PM (PM(2.5-10)) size fractions on pulmonary and cardiac function. Female BALB/c mice were exposed to HEPA-filtered laboratory air or concentrated coarse, fine, or quasi-ultrafine PM using Harvard Ambient Particle Concentrators in conjunction with our nose-only exposure system. These exposures were conducted as part of the "Health Effects of Aerosols in Toronto (HEAT)" campaign. Following a 4 h exposure, mice underwent assessment of respiratory function and recording of electrocardiograms using the flexiVent® system. Exposure to coarse and fine PM resulted in a significant reduction in quasistatic compliance of the lung. Baseline total respiratory resistance and maximum responsiveness to methacholine were augmented after coarse PM exposures but were not affected by quasi-ultrafine PM exposures. In contrast, quasi-ultrafine PM alone had a significant effect on heart rate and in reducing heart rate variability. These findings indicate that coarse and fine PM influence lung function and airways responsiveness, while ultrafine PM can perturb cardiac function. This study supports the hypothesis that coarse and fine PM exerts its predominant physiologic effects at the site of deposition in the airways, whereas ultrafine PM likely crosses the alveolar epithelial barrier into the systemic circulation to affect cardiovascular function.

40 CFR 63.11507 - What are my standards and management practices?

Code of Federal Regulations, 2011 CFR

2011-07-01

... distilled/de-ionized water, water filtration, pre-cleaning of parts to be plated, and thorough rinsing of... emissions from the thermal spraying process and transports the emissions to a water curtain, fabric filter... and transports the emissions to a water curtain, fabric filter, cartridge, or HEPA filter, according...

Transmission Electron Microscopy of Vacuum Sensitive, Radiation Sensitive, and Structurally Delicate Materials

NASA Astrophysics Data System (ADS)

Levin, Barnaby

The transmission electron microscope (TEM) is a powerful tool for characterizing the nanoscale and atomic structure of materials, offering insights into their fundamental physical properties. However, TEM characterization requires very thin samples of material to be placed in a high vacuum environment, and exposed to electron radiation. The high vacuum will induce some materials to evaporate or sublimate, preventing them from being accurately characterized, radiation may damage the sample, causing mass loss, or altering its structure, and structurally delicate samples may collapse and break apart when they are thinned for TEM imaging. This dissertation discusses three different projects in which each of these three difficulties pose challenges to TEM characterization of samples. Firstly, we outline strategies for minimizing radiation damage when characterizing materials in TEM at atomic resolution. We consider types of radiation damage, such as vacancy enhanced displacement, that are not included in some previous discussions of beam damage, and we consider how to minimize damage when using new imaging techniques such as annular bright-field scanning TEM. Our methodology emphasizes the general principle that variation of both signal strength and damage cross section must be considered when choosing an experimental electron beam voltage to minimize damage. Secondly, we consider samples containing sulfur, which is prone to sublimation in high vacuum. TEM is routinely used to attempt to characterize the sulfur distribution in lithium-sulfur battery electrodes, but sublimation artifacts can give misleading results. We demonstrate that sulfur sublimation can be suppressed by using cryogenic TEM to characterize sulfur at very low temperatures, or by using the recently developed airSEM to characterize sulfur without exposing it to vacuum. Finally, we discuss the characterization of aging cadmium yellow paint from early 20th century art masterpieces. The binding medium holding paint particles together bends and curls as sample thickness is reduced to 100 nm, making high resolution characterization challenging. We acquire lattice resolution images of the pigment particles through the binder using high voltage zero-loss energy filtered TEM, allowing us to measure the pigment particle size and determine the pigment crystal structure, providing insight into why the paint is aging and how it was synthesized.

A characterization NMR of secondary metabolites from lichen Parmotrema praesorediosum

NASA Astrophysics Data System (ADS)

Azman, Anis Asmi; Khalid, Rozida; Bakar, Muntaz Abu

2018-04-01

The research study was carried out to extract, isolate and characterize the secondary metabolites of lichen Parmotrema praesorediosum. Most of the lichen samples were obtained from betel nut trees and needle flowers which were collected from 17 different places around UKM Bangi campus. Each lichen sample was dried before being grinded and extracted in methanol for nine days. This process was repeated three times at room temperature. Subsequently, the resulting residues were filtered to obtain the crude extracts and further analysed using Thin Layer Chromatography (TLC) and Vacuum Column Chromatography (VLC). In order to derive the pure compounds, the isolation step was proceeded using Radial Chromatography (RC). These isolated compounds were determined by Nuclear Magnetic Resonances (NMR) and identified as methyl haematomatte (1), methyl chlorohaematomatte (2) and methyl β-orsellinate (3).

Making Healthy Eating and Physical Activity Policy Practice: The Design and Overview of a Group Randomized Controlled Trial in Afterschool Programs

PubMed Central

Beets, Michael W.; Weaver, R. Glenn; Turner-McGrievy, Gabrielle; Huberty, Jennifer; Ward, Dianne S.; Freedman, Darcy A.; Saunders, Ruth; Pate, Russell R.; Beighle, Aaron; Moore, Justin B.

2014-01-01

National and state organizations have developed policies calling upon afterschool programs (ASPs, 3-6pm) to serve a fruit or vegetable (FV) each day for snack, while eliminating foods and beverages high in added-sugars, and to ensure children accumulate a minimum of 30 min/d of moderate-to-vigorous physical activity (MVPA). Few efficacious and cost-effective strategies exist to assist ASP providers in achieving these important public health goals. This paper reports on the design and conceptual framework of Making Healthy Eating and Physical Activity (HEPA) Policy Practice in ASPs, a 3-year group randomized controlled trial testing the effectiveness of strategies designed to improve snacks served and increase MVPA in children attending community-based ASPs. Twenty ASPs, serving over 1,800 children (6-12yrs) will be enrolled and match-paired based on enrollment size, average daily min/d MVPA, and days/week FV served, with ASPs randomized after baseline data collection to immediate intervention or a 1-year delayed group. The framework employed, STEPs (Strategies To Enhance Practice), focuses on intentional programming of HEPA in each ASPs’ daily schedule, and includes a grocery store partnership to reduce price barriers to purchasing FV, professional development training to promote physical activity to develop core physical activity competencies, as well as ongoing technical support/assistance. Primary outcome measures include children’s accelerometry-derived MVPA and time spend sedentary while attending an ASP, direct observation of staff HEPA promoting and inhibiting behaviors, types of snacks served, and child consumption of snacks, as well as, cost of snacks via receipts and detailed accounting of intervention delivery costs to estimate cost-effectiveness. PMID:24893225

Calculation of intrinsic stresses in the diamond-like coatings produced by plasma ion deposition in modes of DC and pulse bias potentials

NASA Astrophysics Data System (ADS)

Kalinichenko, A. A.; Perepelkin, S. S.; Strel'nitskij, V. E.

2015-04-01

The formula derivation for calculation of intrinsic stress in diamond-like coatings deposited from the ion flux in modes of continuous and pulsed potentials in view of process of defects formation is given. The criterion of applicability of obtained formula allowing to determine critical parameters of the pulsed potential mode is suggested. Results of calculation of stresses in diamond-like coatings at deposition of low-energy ions C+ from filtered vacuum arc plasma are adduced. The influence of the bias potential, repetition frequency and pulse duration, on the value of intrinsic stress is discussed. Qualitative agreement of calculated stress and experimental data is stated. The important role of deposition temperature in control of intrinsic stress in deposited coating is noted.

10 CFR 850.3 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

..., that has end-use functions that depend in whole or in part on its shape or design during end use, and... Element delegates his or her functions under this part. High-efficiency particulate air (HEPA) filter... in this part: Action level means the level of airborne concentration of beryllium established...

10 CFR 850.3 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

..., that has end-use functions that depend in whole or in part on its shape or design during end use, and... Element delegates his or her functions under this part. High-efficiency particulate air (HEPA) filter... in this part: Action level means the level of airborne concentration of beryllium established...

Evaluation of the ICET Test Stand to Assess the Performance of a Range of Ceramic Media Filter Elements in Support of ASME AG-1 Subsection FO

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schemmel, A.

High Efficiency Particulate Air (HEPA) filters are defined as extended-medium, dry-type filters with: (1) a minimum particle removal efficiency of no less than 99.97 percent for 0.3 micrometer particles, (2) a maximum, clean resistance of 1.0 inch water column (in. WC) when operated at 1,000 cubic feet per minute (CFM), and (3) a rigid casing that extends the full depth of the medium. Specifically, ceramic media HEPA filters provide better performance at elevated temperatures, are moisture resistant and nonflammable, can perform their function if wetted and exposed to greater pressures, and can be cleaned and reused. This paper describes themore » modification and design of a large scale test stand which properly evaluates the filtration characteristics of a range of ceramic media filters challenged with a nuclear aerosol agent in order to develop Section FO of ASME AG-1.« less

Air cleaning technologies: an evidence-based analysis.

PubMed

2005-01-01

This health technology policy assessment will answer the following questions: When should in-room air cleaners be used?How effective are in-room air cleaners?Are in-room air cleaners that use combined HEPA and UVGI air cleaning technology more effective than those that use HEPA filtration alone?What is the Plasmacluster ion air purifier in the pandemic influenza preparation plan?The experience of severe acute respiratory syndrome (SARS) locally, nationally, and internationally underscored the importance of administrative, environmental, and personal protective infection control measures in health care facilities. In the aftermath of the SARS crisis, there was a need for a clearer understanding of Ontario's capacity to manage suspected or confirmed cases of airborne infectious diseases. In so doing, the Walker Commission thought that more attention should be paid to the potential use of new technologies such as in-room air cleaning units. It recommended that the Medical Advisory Secretariat of the Ontario Ministry of Health and Long-Term Care evaluate the appropriate use and effectiveness of such new technologies. Accordingly, the Ontario Health Technology Advisory Committee asked the Medical Advisory Secretariat to review the literature on the effectiveness and utility of in-room air cleaners that use high-efficiency particle air (HEPA) filters and ultraviolet germicidal irradiation (UVGI) air cleaning technology. Additionally, the Ontario Health Technology Advisory Committee prioritized a request from the ministry's Emergency Management Unit to investigate the possible role of the Plasmacluster ion air purifier manufactured by Sharp Electronics Corporation, in the pandemic influenza preparation plan. Airborne transmission of infectious diseases depends in part on the concentration of breathable infectious pathogens (germs) in room air. Infection control is achieved by a combination of administrative, engineering, and personal protection methods. Engineering methods that are usually carried out by the building's heating, ventilation, and air conditioning (HVAC) system function to prevent the spread of airborne infectious pathogens by diluting (dilution ventilation) and removing (exhaust ventilation) contaminated air from a room, controlling the direction of airflow and the air flow patterns in a building. However, general wear and tear over time may compromise the HVAC system's effectiveness to maintain adequate indoor air quality. Likewise, economic issues may curtail the completion of necessary renovations to increase its effectiveness. Therefore, when exposure to airborne infectious pathogens is a risk, the use of an in-room air cleaner to reduce the concentration of airborne pathogens and prevent the spread of airborne infectious diseases has been proposed as an alternative to renovating a HVAC system. Airborne transmission is the spread of infectious pathogens over large distances through the air. Infectious pathogens, which may include fungi, bacteria, and viruses, vary in size and can be dispersed into the air in drops of moisture after coughing or sneezing. Small drops of moisture carrying infectious pathogens are called droplet nuclei. Droplet nuclei are about 1 to 5μm in diameter. This small size in part allows them to remain suspended in the air for several hours and be carried by air currents over considerable distances. Large drops of moisture carrying infectious pathogens are called droplets. Droplets being larger than droplet nuclei, travel shorter distances (about 1 metre) before rapidly falling out of the air to the ground. Because droplet nuclei remain airborne for longer periods than do droplets, they are more amenable to engineering infection control methods than are droplets. Droplet nuclei are responsible for the airborne transmission of infectious diseases such as tuberculosis, chicken pox (varicella), measles (rubeola), and dessiminated herpes zoster, whereas close contact is required for the direct transmission of infectious diseases transmitted by droplets, such as influenza (the flu) and SARS. In-room air cleaners are supplied as portable or fixed devices. Fixed devices can be attached to either a wall or ceiling and are preferred over portable units because they have a greater degree of reliability (if installed properly) for achieving adequate room air mixing and airflow patterns, which are important for optimal effectiveness. Through a method of air recirculation, an in-room air cleaner can be used to increase room ventilation rates and if used to exhaust air out of the room it can create a negative-pressure room for airborne infection isolation (AII) when the building's HVAC system cannot do so. A negative-pressure room is one where clean air flows into the room but contaminated air does not flow out of it. Contaminated room air is pulled into the in-room air cleaner and cleaned by passing through a series of filters, which remove the airborne infectious pathogens. The cleaned air is either recirculated into the room or exhausted outside the building. By filtering contaminated room air and then recirculating the cleaned air into the room, an in-room air cleaner can improve the room's ventilation. By exhausting the filtered air to the outside the unit can create a negative-pressure room. There are many types of in-room air cleaners. They vary widely in the airflow rates through the unit, the type of air cleaning technology used, and the technical design. Crucial to maximizing the efficiency of any in-room air cleaner is its strategic placement and set-up within a room, which should be done in consultation with ventilation engineers, infection control experts, and/or industrial hygienists. A poorly positioned air cleaner may disrupt airflow patterns within the room and through the air cleaner, thereby compromising its air cleaning efficiency. The effectiveness of an in-room air cleaner to remove airborne pathogens from room air depends on several factors, including the airflow rate through the unit's filter and the airflow patterns in the room. Tested under a variety of conditions, in-room air cleaners, including portable or ceiling mounted units with either a HEPA or a non-HEPA filter, portable units with UVGI lights only, or ceiling mounted units with combined HEPA filtration and UVGI lights, have been estimated to be between 30% and 90%, 99% and 12% and 80% effective, respectively. However, and although their effectiveness is variable, the United States Centers for Disease Control and Prevention has acknowledged in-room air cleaners as alternative technology for increasing room ventilation when this cannot be achieved by the building's HVAC system with preference given to fixed recirculating systems over portable ones. Importantly, the use of an in-room air cleaner does not preclude either the need for health care workers and visitors to use personal protective equipment (N95 mask or equivalent) when entering AII rooms or health care facilities from meeting current regulatory requirements for airflow rates (ventilation rates) in buildings and airflow differentials for effective negative-pressure rooms. The Plasmacluster ion technology, developed in 2000, is an air purification technology. Its manufacturer, Sharp Electronics Corporation, says that it can disable airborne microorganisms through the generation of both positive and negative ions. (1) The functional unit is the hydroxyl, which is a molecule comprised of one oxygen molecule and one hydrogen atom. Plasmacluster ion air purifier uses a multilayer filter system composed of a prefilter, a carbon filter, an antibacterial filter, and a HEPA filter, combined with an ion generator to purify the air. The ion generator uses an alternating plasma discharge to split water molecules into positively and negatively charged ions. When these ions are emitted into the air, they are surrounded by water molecules and form cluster ions which are attracted to airborne particles. The cluster ion surrounds the airborne particle, and the positive and negative ions react to form hydroxyls. These hydroxyls steal the airborne particle's hydrogen atom, which creates a hole in the particle's outer protein membrane, thereby rendering it inactive. Because influenza is primarily acquired by large droplets and direct and indirect contact with an infectious person, any in-room air cleaner will have little benefit in controlling and preventing its spread. Therefore, there is no role for the Plasmacluster ion air purifier or any other in-room air cleaner in the control of the spread of influenza. Accordingly, for purposes of this review, the Medical Advisory Secretariat presents no further analysis of the Plasmacluster. The objective of the systematic review was to determine the effectiveness of in-room air cleaners with built in UVGI lights and HEPA filtration compared with those using HEPA filtration only. The Medical Advisory Secretariat searched the databases of MEDLINE, EMBASE, Cochrane Database of Systematic Reviews, INAHATA (International Network of Agencies for Health Technology Assessment), Biosis Previews, Bacteriology Abstracts, Web of Science, Dissertation Abstracts, and NIOSHTIC 2. A meta-analysis was conducted if adequate data was available from 2 or more studies and where statistical and clinical heterogeneity among studies was not an issue. Otherwise, a qualitative review was completed. The GRADE system was used to summarize the quality of the body of evidence comprised of 1 or more studies. There were no existing health technology assessments on air cleaning technology located during the literature review. The literature search yielded 59 citations of which none were retained. (ABSTRACT TRUNCATED)

40 CFR 721.10572 - Benzamide, N-[[4- [(cyclopropylamino)carbonyl] phenyl]sulfonyl]-2-methoxy-.

Code of Federal Regulations, 2014 CFR

2014-07-01

...-fitting half-face respirator equipped with N100 (if oil aerosols absent), R100, or P100 filters. (B) NIOSH-certified air-purifying, tight-fitting full-face respirator equipped with N100 (if oil aerosols absent...-purifying respirator equipped with a tight-fitting facepiece (either half-face or full-face) and HEPA...

Analysis of pharmaceutical and other organic wastewater compounds in filtered and unfiltered water samples by gas chromatography/mass spectrometry

USGS Publications Warehouse

Zaugg, Steven D.; Phillips, Patrick J.; Smith, Steven G.

2014-01-01

Research on the effects of exposure of stream biota to complex mixtures of pharmaceuticals and other organic compounds associated with wastewater requires the development of additional analytical capabilities for these compounds in water samples. Two gas chromatography/mass spectrometry (GC/MS) analytical methods used at the U.S. Geological Survey National Water Quality Laboratory (NWQL) to analyze organic compounds associated with wastewater were adapted to include additional pharmaceutical and other organic compounds beginning in 2009. This report includes a description of method performance for 42 additional compounds for the filtered-water method (hereafter referred to as the filtered method) and 46 additional compounds for the unfiltered-water method (hereafter referred to as the unfiltered method). The method performance for the filtered method described in this report has been published for seven of these compounds; however, the addition of several other compounds to the filtered method and the addition of the compounds to the unfiltered method resulted in the need to document method performance for both of the modified methods. Most of these added compounds are pharmaceuticals or pharmaceutical degradates, although two nonpharmaceutical compounds are included in each method. The main pharmaceutical compound classes added to the two modified methods include muscle relaxants, opiates, analgesics, and sedatives. These types of compounds were added to the original filtered and unfiltered methods largely in response to the tentative identification of a wide range of pharmaceutical and other organic compounds in samples collected from wastewater-treatment plants. Filtered water samples are extracted by vacuum through disposable solid-phase cartridges that contain modified polystyrene-divinylbenzene resin. Unfiltered samples are extracted by using continuous liquid-liquid extraction with dichloromethane. The compounds of interest for filtered and unfiltered sample types were determined by use of the capillary-column gas chromatography/mass spectrometry. The performance of each method was assessed by using data on recoveries of compounds in fortified surface-water, wastewater, and reagent-water samples. These experiments (referred to as spike experiments) consist of fortifying (or spiking) samples with known amounts of target analytes. Surface-water-spike experiments were performed by using samples obtained from a stream in Colorado (unfiltered method) and a stream in New York (filtered method). Wastewater spike experiments for both the filtered and unfiltered methods were performed by using a treated wastewater obtained from a single wastewater treatment plant in New York. Surface water and wastewater spike experiments were fortified at both low and high concentrations and termed low- and high-level spikes, respectively. Reagent water spikes were assessed in three ways: (1) set spikes, (2) a low-concentration fortification experiment, and (3) a high-concentration fortification experiment. Set spike samples have been determined since 2009, and consist of analysis of fortified reagent water for target compounds included for each group of 10 to18 environmental samples analyzed at the NWQL. The low-concentration and high-concentration reagent spike experiments, by contrast, represent a one-time assessment of method performance. For each spike experiment, mean recoveries ranging from 60 to 130 percent indicate low bias, and relative standard deviations (RSDs) less than ( Of the compounds included in the filtered method, 21 had mean recoveries ranging from 63 to 129 percent for the low-level and high-level surface-water spikes, and had low ()132 percent]. For wastewater spikes, 24 of the compounds included in the filtered method had recoveries ranging from 61 to 130 percent for the low-level and high-level spikes. RSDs were 130 percent) or variable recoveries (RSDs >30 percent) for low-level wastewater spikes, or low recoveries ( Of the compounds included in the unfiltered method, 17 had mean spike recoveries ranging from 74 to 129 percent and RSDs ranging from 5 to 25 percent for low-level and high-level surface water spikes. The remaining compounds had poor mean recoveries (130 percent), or high RSDs (>29 percent) for these spikes. For wastewater, 14 of the compounds included in the unfiltered method had mean recoveries ranging from 62 to 127 percent and RSDs 130 percent), or low mean recoveries (33 percent) for the low-level wastewater spikes. Of the compounds found in wastewater, 24 had mean set spike recoveries ranging from 64 to 104 percent and RSDs Separate method detection limits (MDLs) were computed for surface water and wastewater for both the filtered and unfiltered methods. Filtered method MDLs ranged from 0.007 to 0.14 microgram per liter (μg/L) for the surface water matrix and from 0.004 to 0.62 μg/L for the wastewater matrix. Unfiltered method MDLs ranged from 0.014 to 0.33 μg/L for the surface water matrix and from 0.008 to 0.36 μg/L for the wastewater matrix.

Comparative Biochemistry and Metabolism. Part 2. Naphthalene Lung Toxicity.

DTIC Science & Technology

1985-11-01

Metabolites to Intra- and Extracellular Proteins .............. ........ 18 Thiol Status in Isolated Hepatocytes Incubated with Naphthalene or Menadione ...Viabilities of isolated hepatocytes incubated with menadione and varying concentrations of n aphthalene...23 15 Intracellular reduced glutathione levels in hepa- tocytes incubated in the presence of menadione or varying concentrations of naphthalene

40 CFR 721.10583 - Benzenamine, 4,4′-[1,3-phenylenebis(1-methylethylidene)]bis-.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., tight-fitting half-face respirator equipped with N100 (if oil aerosols absent), R100, or P100 filters... aerosols absent), R100, or P100 filters. (C) NIOSH-certified powered air-purifying respirator equipped with a loose-fitting hood or helmet and high efficiency particulate air (HEPA) filters. (D) NIOSH...

40 CFR 721.10583 - Benzenamine, 4,4′-[1,3-phenylenebis(1-methylethylidene)]bis-.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., tight-fitting half-face respirator equipped with N100 (if oil aerosols absent), R100, or P100 filters... aerosols absent), R100, or P100 filters. (C) NIOSH-certified powered air-purifying respirator equipped with a loose-fitting hood or helmet and high efficiency particulate air (HEPA) filters. (D) NIOSH...

Upgraded divertor Thomson scattering system on DIII-D

NASA Astrophysics Data System (ADS)

Glass, F.; Carlstrom, T. N.; Du, D.; McLean, A. G.; Taussig, D. A.; Boivin, R. L.

2016-11-01

A design to extend the unique divertor Thomson scattering system on DIII-D to allow measurements of electron temperature and density in high triangularity plasmas is presented. Access to this region is selectable on a shot-by-shot basis by redirecting the laser beam of the existing divertor Thomson system inboard — beneath the lower floor using a moveable, high-damage threshold, in-vacuum mirror — and then redirecting again vertically. The currently measured divertor region remains available with this mirror retracted. Scattered light is collected from viewchords near the divertor floor using in-vacuum, high temperature optical elements and relayed through the port window, before being coupled into optical fiber bundles. At higher elevations from the floor, measurements are made by dynamically re-focusing the existing divertor system collection optics. Nd:YAG laser timing, analysis of the scattered light spectrum via polychromators, data acquisition, and calibration are all handled by existing systems or methods of the current multi-pulse Thomson scattering system. Existing filtered polychromators with 7 spectral channels are employed to provide maximum measurement breadth (Te in the range of 0.5 eV-2 keV, ne in the range of 5 × 1018-1 × 1021 m3) for both low Te in detachment and high Te measurement up beyond the separatrix.

Upgraded divertor Thomson scattering system on DIII-D.

PubMed

Glass, F; Carlstrom, T N; Du, D; McLean, A G; Taussig, D A; Boivin, R L

2016-11-01

A design to extend the unique divertor Thomson scattering system on DIII-D to allow measurements of electron temperature and density in high triangularity plasmas is presented. Access to this region is selectable on a shot-by-shot basis by redirecting the laser beam of the existing divertor Thomson system inboard - beneath the lower floor using a moveable, high-damage threshold, in-vacuum mirror - and then redirecting again vertically. The currently measured divertor region remains available with this mirror retracted. Scattered light is collected from viewchords near the divertor floor using in-vacuum, high temperature optical elements and relayed through the port window, before being coupled into optical fiber bundles. At higher elevations from the floor, measurements are made by dynamically re-focusing the existing divertor system collection optics. Nd:YAG laser timing, analysis of the scattered light spectrum via polychromators, data acquisition, and calibration are all handled by existing systems or methods of the current multi-pulse Thomson scattering system. Existing filtered polychromators with 7 spectral channels are employed to provide maximum measurement breadth (T e in the range of 0.5 eV-2 keV, n e in the range of 5 × 10 18 -1 × 10 21 m 3 ) for both low T e in detachment and high T e measurement up beyond the separatrix.

Decontamination of indoor air to reduce the risk of airborne infections: Studies on survival and inactivation of airborne pathogens using an aerobiology chamber.

PubMed

Sattar, Syed A; Kibbee, Richard J; Zargar, Bahram; Wright, Kathryn E; Rubino, Joseph R; Ijaz, M Khalid

2016-10-01

Although indoor air can spread many pathogens, information on the airborne survival and inactivation of such pathogens remains sparse. Staphylococcus aureus and Klebsiella pneumoniae were nebulized separately into an aerobiology chamber (24.0 m 3 ). The chamber's relative humidity and air temperature were at 50% ± 5% and 20°C ± 2°C, respectively. The air was sampled with a slit-to-agar sampler. Between tests, filtered air purged the chamber of any residual airborne microbes. The challenge in the air varied between 4.2 log 10 colony forming units (CFU)/m 3 and 5.0 log 10 CFU/m 3 , sufficient to show a ≥3 log 10 (≥99.9%) reduction in microbial viability in air over a given contact time by the technologies tested. The rates of biologic decay of S aureus and K pneumoniae were 0.0064 ± 0.00015 and 0.0244 ± 0.009 log 10 CFU/m 3 /min, respectively. Three commercial devices, with ultraviolet light and HEPA (high-efficiency particulate air) filtration, met the product efficacy criterion in 45-210 minutes; these rates were statistically significant compared with the corresponding rates of biologic decay of the bacteria. One device was also tested with repeated challenges with aerosolized S aureus to simulate ongoing fluctuations in indoor air quality; it could reduce each such recontamination to an undetectable level in approximately 40 minutes. The setup described is suitable for work with all major classes of pathogens and also complies with the U.S. Environmental Protection Agency's guidelines (2012) for testing air decontamination technologies. Copyright © 2016 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

Activation of aryl hydrocarbon receptor reduces carbendazim-induced cell death

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wei, Kuo-Liang

Carbendazim inhibits microtubule assembly, thus blocking mitosis and inhibiting cancer cell proliferation. Accordingly, carbendazim is being explored as an anticancer drug. Data show that carbendazim increased mRNA and protein expressions and promoter activity of CYP1A1. In addition, carbendazim activated transcriptional activity of the aryl hydrocarbon response element, and induced nuclear translocation of the aryl hydrocarbon receptor (AhR), a sign the AhR is activated. Carbendazim-induced CYP1A1 expression was blocked by AhR antagonists, and was abolished in AhR signal-deficient cells. Results demonstrated that carbendazim activated the AhR, thereby stimulating CYP1A1 expression. In order to understand whether AhR-induced metabolic enzymes turn carbendazim intomore » less-toxic metabolites, Hoechst 33342 staining to reveal carbendazim-induced nuclear changes and flow cytometry to reveal the subG{sub 0}/G{sub 1} population were applied to monitor carbendazim-induced cell apoptosis. Carbendazim induced less apoptosis in Hepa-1c1c7 cells than in AhR signal-deficient Hepa-1c1c7 mutant cells. Pretreatment with β-NF, an AhR agonist that highly induces CYP1A1 expression, decreased carbendazim-induced cell death. In addition, the lower the level of AhR was, the lower the vitality present in carbendazim-treated cells, including hepatoma cells and their derivatives with AhR RNA interference, also embryonic kidney cells, bladder carcinoma cells, and AhR signal-deficient Hepa-1c1c7 cells. In summary, carbendazim is an AhR agonist. The toxicity of carbendazim was lower in cells with the AhR signal. This report provides clues indicating that carbendazim is more potent at inducing cell death in tissues without than in those with the AhR signal, an important reference for applying carbendazim in cancer chemotherapy. - Highlights: • Carbendazim induced transcriptional activity of the aryl hydrocarbon response element. • Carbendazim induced nuclear translocation of the aryl hydrocarbon receptor (AhR). • Carbendazim induced the expression of cytochrome P450 (CYP) 1A1, an AhR-targeted gene. • Carbendazim is an AhR agonist. • The activated AhR decreased carbendazim-induced cell toxicity and death.« less

Mold Species in Dust from the International Space Station Identified and Quantified by Mold Specific Quantitative PCR

EPA Science Inventory

Dust was collected over a period of several weeks in 2007 from HEPA filters in the U.S. Laboratory Module of the International Space Station (ISS). The dust was returned on the Space Shuttle Atlantis, mixed, sieved, and the DNA was extracted. Using a DNA-based method called mol...

Military Vaccines in Today’s Environment

DTIC Science & Technology

2012-08-01

vaccines for anthrax, plague, influenza, rubella, ade- noviruses, meningococci, hepatitis B, typhoid , Japanese encephalitis, and hepa- titis A...licensed vaccines for naturally occurring diseases, such as those for yellow fever , mumps, measles, chickenpox and polio, were developed with the...HIV-AIDS, Chikungunya, Rift Valley fever , Argentinian hemorrhagic fever , and hemorrhagic fever with renal syndrome (HFRS), have been developed and

High-efficiency particulate air filter test stand and aerosol generator for particle loading studies.

PubMed

Arunkumar, R; Hogancamp, Kristina U; Parsons, Michael S; Rogers, Donna M; Norton, Olin P; Nagel, Brian A; Alderman, Steven L; Waggoner, Charles A

2007-08-01

This manuscript describes the design, characterization, and operational range of a test stand and high-output aerosol generator developed to evaluate the performance of 30 x 30 x 29 cm(3) nuclear grade high-efficiency particulate air (HEPA) filters under variable, highly controlled conditions. The test stand system is operable at volumetric flow rates ranging from 1.5 to 12 standard m(3)/min. Relative humidity levels are controllable from 5%-90% and the temperature of the aerosol stream is variable from ambient to 150 degrees C. Test aerosols are produced through spray drying source material solutions that are introduced into a heated stainless steel evaporation chamber through an air-atomizing nozzle. Regulation of the particle size distribution of the aerosol challenge is achieved by varying source solution concentrations and through the use of a postgeneration cyclone. The aerosol generation system is unique in that it facilitates the testing of standard HEPA filters at and beyond rated media velocities by consistently providing, into a nominal flow of 7 standard m(3)/min, high mass concentrations (approximately 25 mg/m(3)) of dry aerosol streams having count mean diameters centered near the most penetrating particle size for HEPA filters (120-160 nm). Aerosol streams that have been generated and characterized include those derived from various concentrations of KCl, NaCl, and sucrose solutions. Additionally, a water insoluble aerosol stream in which the solid component is predominantly iron (III) has been produced. Multiple ports are available on the test stand for making simultaneous aerosol measurements upstream and downstream of the test filter. Types of filter performance related studies that can be performed using this test stand system include filter lifetime studies, filtering efficiency testing, media velocity testing, evaluations under high mass loading and high humidity conditions, and determination of the downstream particle size distributions.

Cytochrome P450 2A5 and bilirubin: Mechanisms of gene regulation and cytoprotection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Sangsoo Daniel; Antenos, Monica; Squires, E. James

2013-07-15

Bilirubin (BR) has recently been identified as the first endogenous substrate for cytochrome P450 2A5 (CYP2A5) and it has been suggested that CYP2A5 plays a major role in BR clearance as an alternative mechanism to BR conjugation by uridine-diphosphate glucuronyltransferase 1A1. This study investigated the mechanisms of Cyp2a5 gene regulation by BR and the cytoprotective role of CYP2A5 in BR hepatotoxicity. BR induced CYP2A5 expression at the mRNA and protein levels in a dose-dependent manner in primary mouse hepatocytes. BR treatment also caused nuclear translocation of Nuclear factor-E2 p45-related factor 2 (Nrf2) in hepatocytes. In reporter assays, BR treatment ofmore » primary hepatocytes transfected with a Cyp2a5 promoter-luciferase reporter construct resulted in a 2-fold induction of Cyp2a5 reporter activity. Furthermore, cotransfection of the hepatocytes with a Nrf2 expression vector without BR treatment resulted in an increase in Cyp2a5 reporter activity of approximately 2-fold and BR treatment of Nrf2 cotransfectants further increased reporter activity by 4-fold. In addition, site-directed mutation of the ARE in the reporter construct completely abolished both the BR- and Nrf2-mediated increases in reporter activity. The cytoprotective role of CYP2A5 against BR-mediated apoptosis was also examined in Hepa 1–6 cells that lack endogenous CYP2A5. Transient overexpression of CYP2A5 partially blocked BR-induced caspase-3 cleavage in Hepa 1–6 cells. Furthermore, in vitro degradation of BR was increased by microsomes from Hepa 1–6 cells overexpressing CYP2A5 compared to control cells transfected with an empty vector. Collectively, these results suggest that Nrf2-mediated CYP2A5 transactivation in response to BR may provide an additional mechanism for adaptive cytoprotection against BR hepatotoxicity. - Highlights: • The mechanism of Cyp2a5 gene regulation by BR was investigated. • The cytoprotective role of CYP2A5 in BR hepatotoxicity was determined. • BR induces CYP2A5 mRNA and protein expression. • BR increases CYP2A5 transcription via Nrf2 activation. • CYP2A5 overexpression increases BR clearance and reduces caspase-3 activation.« less

X-Ray Radiation Measurements With Photodiodes In Plasmas Generated By 1017 W/Cm2 Intensity Krf Excimer Laser Pulses

NASA Astrophysics Data System (ADS)

Rácz, E.; Földes, I. B.; Ryć, L.

2006-01-01

Experiments were carried out using a prepulse-free hybrid KrF excimer-dye laser system (700fs pulse duration, 248nm wavelength, 15mJ pulse energy). The intensity of the p-polarized, focused laser beam was 1.5ṡ1017 W/cm2. Vacuum ultraviolet (VUV) and x-rays from solid state laser plasmas were generated in the laser-plasma interaction of subpicosecond laser pulses of nonrelativistic laser intensities. An x-ray sensitive FLM photodiode (ITE, Warsaw) was used to detect x-rays between 1-19 keV in front of the targets. The diode was filtered by a 4μm Al foil. The dependence of the x-ray flux on laser intensity and the angular distribution of x-rays for aluminum and copper targets in the half space of the front side of the targets were investigated.

Direct filtration for recovery of Schistosoma mansoni cercariae in the field.

PubMed

Sandt, D G

1973-01-01

The recovery of schistosome cercariae from natural waters has been limited by variations in turbidity and in the accuracy of recovery with different techniques. A modification of the Rowan vacuum paper filtration method employing a battery-operated pumping system, a glass-silicone plate filter, and a specially designed filter holder is described and evaluated. Field tests on St Lucia indicate a mean filtration volume of 12.2 litres per filter at a mean turbidity of 20.3 Jackson turbidity units. Overall, 86% of the volumes filtered per filter were in excess of 6 litres. Particle size, rather than turbidity, was found to be the main factor influencing filter blockage, reading time, and accuracy. Recoveries of 0.01 cercaria (Schistosoma mansoni) per litre sampled were obtained, but the practical limit of the method is considered to be closer to 0.1 cercaria per litre sampled.

Protection of the vehicle cab environment against bacteria, fungi and endotoxins in composting facilities.

PubMed

Schlosser, O; Huyard, A; Rybacki, D; Do Quang, Z

2012-06-01

Microbial quality of air inside vehicle cabs is a major occupational health risk management issue in composting facilities. Large differences and discrepancies in protection factors between vehicles and between biological agents have been reported. This study aimed at estimating the mean protection efficiency of the vehicle cab environment against bioaerosols with higher precision. In-cab measurement results were also analysed to ascertain whether or not these protection systems reduce workers' exposure to tolerable levels. Five front-end loaders, one mobile mixer and two agricultural tractors pulling windrow turners were investigated. Four vehicles were fitted with a pressurisation and high efficiency particulate air (HEPA) filtration system. The four others were only equipped with pleated paper filter without pressurisation. Bacteria, fungi and endotoxins were measured in 72 pairs of air samples, simultaneously collected inside the cab and on the outside of the cab with a CIP 10-M sampler. A front-end loader, purchased a few weeks previously, fitted with a pressurisation and high efficiency particulate air (HEPA) filtration system, and with a clean cab, exhibited a mean protection efficiency of between 99.47% CI 95% [98.58-99.97%] and 99.91% [99.78-99.98%] depending on the biological agent. It is likely that the lower protection efficiency demonstrated in other vehicles was caused by penetration through the only moderately efficient filters, by the absence of pressurisation, by leakage in the filter-sealing system, and by re-suspension of particles which accumulated in dirty cabs. Mean protection efficiency in regards to bacteria and endotoxins ranged between 92.64% [81.87-97.89%] and 98.61% [97.41-99.38%], and between 92.68% [88.11-96.08%] and 98.43% [97.44-99.22%], respectively. The mean protection efficiency was the lowest when confronted with fungal spores, from 59.76% [4.19-90.75%] to 94.71% [91.07-97.37%]. The probability that in-cab exposure to fungi exceeded the benchmark value for short-term respiratory effects suggests that front-end loaders and mobile mixers in composting facilities should be fitted with a pressurisation and HEPA filtration system, regardless of whether or not the facility is indoors or outdoors. Regarding the tractors, exposure inside the cabs was not significantly reduced. However, in this study, there was a less than 0.01% risk of exceeding the bench mark value associated with fungi related short-term respiratory effects during an 1-h per day windrow turning operation. Pressurisation and a HEPA filtration system can provide safe working conditions inside loaders and mobile mixer with regard to airborne bacteria, fungi and endotoxins in composting facilities. However, regular thorough cleaning of the vehicle cab, as well as overalls and shoes cleaning, and mitigation of leakage in the filter-sealing system are necessary to achieve high levels of protection efficiency. Copyright © 2012 Elsevier Ltd. All rights reserved.

TOXICITY PROFILES IN RATS TREATED WITH TUMORIGENIC AND NONTUMORIGENIC TRIAZOLE CONAZOLE FUNGICIDES: PROPICONAZOLE, TRIADIMEFON, AND MYCLOBUTANIL

EPA Science Inventory

Conazoles are a class of azole based fungicides used in agriculture and as pharmaceutical products. They have a common mode of antifungal action through inhibition of ergosterol biosynthesis. Some members of this class have been shown to be hepatotoxic and will induce mouse hepa...

Method of concurrently filtering particles and collecting gases

DOEpatents

Mitchell, Mark A; Meike, Annemarie; Anderson, Brian L

2015-04-28

A system for concurrently filtering particles and collecting gases. Materials are be added (e.g., via coating the ceramic substrate, use of loose powder(s), or other means) to a HEPA filter (ceramic, metal, or otherwise) to collect gases (e.g., radioactive gases such as iodine). The gases could be radioactive, hazardous, or valuable gases.

40 CFR 721.10575 - 1-Propanone, 1,1'-(oxydi-4,1-phenylene)bis[2-hydroxy-2-methyl-.

Code of Federal Regulations, 2013 CFR

2013-07-01

...), R100, or P100 filters. (B) NIOSH-certified air-purifying, tight-fitting full-face respirator equipped with N100 (if oil aerosols absent), R100, or P100 filters. (C) NIOSH-certified powered air-purifying respirator equipped with a loose-fitting hood or helmet and high efficiency particulate air (HEPA) filters...

40 CFR 721.10575 - 1-Propanone, 1,1'-(oxydi-4,1-phenylene)bis[2-hydroxy-2-methyl-.

Code of Federal Regulations, 2014 CFR

2014-07-01

...), R100, or P100 filters. (B) NIOSH-certified air-purifying, tight-fitting full-face respirator equipped with N100 (if oil aerosols absent), R100, or P100 filters. (C) NIOSH-certified powered air-purifying respirator equipped with a loose-fitting hood or helmet and high efficiency particulate air (HEPA) filters...

Demolition of a hospital building by controlled explosion: the impact on filamentous fungal load in internal and external air.

PubMed

Bouza, E; Peláez, T; Pérez-Molina, J; Marín, M; Alcalá, L; Padilla, B; Muñoz, P; Adán, P; Bové, B; Bueno, M J; Grande, F; Puente, D; Rodríguez, M P; Rodríguez-Créixems, M; Vigil, D; Cuevas, O

2002-12-01

The demolition of a maternity building at our institution provided us with the opportunity to study the load of filamentous fungi in the air. External (nearby streets) and internal (within the hospital buildings) air was sampled with an automatic volumetric machine (MAS-100 Air Samplair) at least daily during the week before the demolition, at 10, 30, 60, 90,120, 180, 240, 420, 540 and 660 min post-demolition, daily during the week after the demolition and weekly during weeks 2, 3 and 4 after demolition. Samples were duplicated to analyse reproducibility. Three hundred and forty samples were obtained: 115 external air, 69 'non-protected' internal air and 156 protected internal air [high efficiency particulate air (HEPA) filtered air under positive pressure]. A significant increase in the colony count of filamentous fungi occurred after the demolition. Median colony counts of external air on demolition day were significantly higher than from internal air (70.2 cfu/m(3) vs 35.8 cfu/m(3)) (P < 0.001). Mechanical demolition on day +4 also produced a significant difference between external and internal air (74.5 cfu/m(3) vs 41.7 cfu/m(3)). The counts returned to baseline levels on day +11. Most areas with a protected air supply yielded no colonies before demolition day and remained negative on demolition day. The reproducibility of the count method was good (intra-assay variance: 2.4 cfu/m(3)). No episodes of invasive filamentous mycosis were detected during the three months following the demolition. Demolition work was associated with a significant increase in the fungal colony counts of hospital external and non-protected internal air. Effective protective measures may be taken to avoid the emergence of clinical infections. Copyright 2002 The Hospital Infection Society

Zink rotary kiln seal: Cam followers. Revision 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fisher, D.L.

1994-12-09

The CIF will treat hazardous and mixed low-level radioactive waste in a rotary kiln and secondary combustion chamber. A high efficiency air pollution control system follows the secondary chamber. The rotary kiln is designed with a gas seal at each end of its rotating barrel which provides a barrier between the interior of the kiln and outside air. The internal pressure of the rotary kiln will be maintained below atmospheric pressure, so exterior air passing the seals is forced into the kiln`s interior. Positive pressure may be applied in the seal labyrinth, adding a barrier to flow. Both CIF sealsmore » will be covered entirely with exhaust hoods, drawing air over the outside of the seal and into a HEPA filtered exhaust system. Cam follower misalignment on a John Zink rotary kiln seal caused damage to the seal`s rotor. The misalignment was quantified, corrected, and checked to verify straightness. The primary purpose of the correction was to allow seal testing 1 to continue, but the information is applicable to the Consolidated Incineration Facility (CIF) since two large seals of similar design will be installed there. Cam follower straightness was off as much as 3.5{degrees}, causing followers to run untrue on the rotor. High contact forces resulted, removing flakes of metal from the rotor surface. The misalignment caused weight bearing followers on one side of the seal to back out of their threaded mounts. The root cause was poor machining of the follower mounting holes. Correction was accomplished by relieving the holes and installing machined spacers and retaining nuts. Cam followers on the CIF`s Zink seals should be inspected for straightness before the seals are installed.« less

CBR/TIC Filter Design and Evaluation

DTIC Science & Technology

2006-12-29

CBR/TIC Filters Aged CK Filter Life Predicitions.................................................................. 29 Figure 30. CBR/TIC Filters...potting adhesive. Neoprene rubber face gaskets are adhered to each end of the particulate filter for airtight sealing. Figure 16. CBR/TIC HEPA...attached with adhesive to the bottom end cover and is spaced by the compression pads on the top end. EPDM foam compression pads are placed on top

Ecofriendly Fruit Switches: Graphene Oxide-Based Wrapper for Programmed Fruit Preservative Delivery To Extend Shelf Life.

PubMed

Sharma, Sandeep; Biswal, Badal Kumar; Kumari, Divya; Bindra, Pulkit; Kumar, Satish; Stobdan, Tsering; Shanmugam, Vijayakumar

2018-05-21

According to Food and Agriculture Organization 2015 report, post-harvest agricultural loss accounts for 20-50% annually; on the other hand, reports about preservatives toxicity are also increasing. Hence, preservative release with response to fruit requirement is desired. In this study, acid synthesized in the overripe fruits was envisaged to cleave acid labile hydrazone to release preservative salicylaldehyde from graphene oxide (GO). To maximize loading and to overcome the challenge of GO reduction by hydrazine, two-step activation with ethylenediamine and 4-nitrophenyl chloroformate respectively, are followed. The final composite shows efficient preservative release with the stimuli of the overripe fruit juice and improves the fruit shelf life. The composite shows less toxicity as compared to the free preservative along with the additional scope to reuse. The composite was vacuum-filtered through a 0.4 μm filter paper, to prepare a robust wrapper for the fruit storage.

Structural characteristics of surface-functionalized nitrogen-doped diamond-like carbon films and effective adjustment to cell attachment

NASA Astrophysics Data System (ADS)

Liu, Ai-Ping; Liu, Min; Yu, Jian-Can; Qian, Guo-Dong; Tang, Wei-Hua

2015-05-01

Nitrogen-doped diamond-like carbon (DLC:N) films prepared by the filtered cathodic vacuum arc technology are functionalized with various chemical molecules including dopamine (DA), 3-Aminobenzeneboronic acid (APBA), and adenosine triphosphate (ATP), and the impacts of surface functionalities on the surface morphologies, compositions, microstructures, and cell compatibility of the DLC:N films are systematically investigated. We demonstrate that the surface groups of DLC:N have a significant effect on the surface and structural properties of the film. The activity of PC12 cells depends on the particular type of surface functional groups of DLC:N films regardless of surface roughness and wettability. Our research offers a novel way for designing functionalized carbon films as tailorable substrates for biosensors and biomedical engineering applications. Project supported by the National Natural Science Foundation of China (Grant Nos. 51272237, 51272231, and 51010002) and the China Postdoctoral Science Foundation (Grant Nos. 2012M520063, 2013T60587, and Bsh1201016).

Formation of (Ti,Al)N/Ti{sub 2}AlN multilayers after annealing of TiN/TiAl(N) multilayers deposited by ion beam sputtering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dolique, V.; Jaouen, M.; Cabioc'h, T.

2008-04-15

By using ion beam sputtering, TiN/TiAl(N) multilayers of various modulation wavelengths ({lambda}=8, 13, and 32 nm) were deposited onto silicon substrates at room temperature. After annealing at 600 deg. C in vacuum, one obtains for {lambda}=13 nm a (Ti,Al)N/Ti{sub 2}AlN multilayer as it is evidenced from x-ray diffraction, high resolution transmission electron microscopy, and energy filtered electron imaging experiments. X-ray photoelectron spectroscopy (XPS) experiments show that the as-deposited TiAl sublayers contain a noticeable amount of nitrogen atoms which mean concentration varies with the period {lambda}. They also evidenced the diffusion of aluminum into TiN sublayers after annealing. Deduced from thesemore » observations, we propose a model to explain why this solid-state phase transformation depends on the period {lambda} of the multilayer.« less

Mold Species in Dust from the International Space Station Identified and Quantified by Mold Specific Quantitative PCR - MCEARD

EPA Science Inventory

Dust was collected over a period of several weeks in 2007 from HEPA filters in the U.S. Laboratory Module of the International Space Station (ISS). The dust was returned on the Space Shuttle Atlantis, mixed, sieved, and the DNA was extracted. Using a DNA-based method called mo...

Identifying Strategies Programs Adopt to Meet Healthy Eating and Physical Activity Standards in Afterschool Programs

ERIC Educational Resources Information Center

Weaver, Robert G.; Moore, Justin B.; Turner-McGrievy, Brie; Saunders, Ruth; Beighle, Aaron; Khan, M. Mahmud; Chandler, Jessica; Brazendale, Keith; Randell, Allison; Webster, Collin; Beets, Michael W.

2017-01-01

Background: The YMCA of USA has adopted Healthy Eating and Physical Activity (HEPA) Standards for its afterschool programs (ASPs). Little is known about strategies YMCA ASPs are implementing to achieve Standards and these strategies' effectiveness. Aims: (1) Identify strategies implemented in YMCA ASPs and (2) evaluate the relationship between…

Home interventions are effective at decreasing indoor nitrogen dioxide concentrations.

PubMed

Paulin, L M; Diette, G B; Scott, M; McCormack, M C; Matsui, E C; Curtin-Brosnan, J; Williams, D L; Kidd-Taylor, A; Shea, M; Breysse, P N; Hansel, N N

2014-08-01

Nitrogen dioxide (NO2 ), a by-product of combustion produced by indoor gas appliances such as cooking stoves, is associated with respiratory symptoms in those with obstructive airways disease. We conducted a three-armed randomized trial to evaluate the efficacy of interventions aimed at reducing indoor NO2 concentrations in homes with unvented gas stoves: (i) replacement of existing gas stove with electric stove; (ii) installation of ventilation hood over existing gas stove; and (iii) placement of air purifiers with high-efficiency particulate air (HEPA) and carbon filters. Home inspection and NO2 monitoring were conducted at 1 week pre-intervention and at 1 week and 3 months post-intervention. Stove replacement resulted in a 51% and 42% decrease in median NO2 concentration at 3 months of follow-up in the kitchen and bedroom, respectively (P = 0.01, P = 0.01); air purifier placement resulted in an immediate decrease in median NO2 concentration in the kitchen (27%, P < 0.01) and bedroom (22%, P = 0.02), but at 3 months, a significant reduction was seen only in the kitchen (20%, P = 0.05). NO2 concentrations in the kitchen and bedroom did not significantly change following ventilation hood installation. Replacing unvented gas stoves with electric stoves or placement of air purifiers with HEPA and carbon filters can decrease indoor NO2 concentrations in urban homes. Several combustion sources unique to the residential indoor environment, including gas stoves, produce nitrogen dioxide (NO2), and higher NO2 concentrations, are associated with worse respiratory morbidity in people with obstructive lung disease. A handful of studies have modified the indoor environment by replacing unvented gas heaters; this study, to our knowledge, is the first randomized study to target unvented gas stoves. The results of this study show that simple home interventions, including replacement of an unvented gas stove with an electric stove or placement of HEPA air purifiers with carbon filters, can significantly decrease indoor NO2 concentrations. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

An Ice Core Melter System for Continuous Major and Trace Chemical Analyses of a New Mt. Logan Summit Ice Core

NASA Astrophysics Data System (ADS)

Osterberg, E. C.; Handley, M. J.; Sneed, S. D.; Mayewski, P. A.; Kreutz, K. J.; Fisher, D. A.

2004-12-01

The ice core melter system at the University of Maine Climate Change Institute has been recently modified and updated to allow high-resolution (<1-2 cm ice/sample), continuous and coregistered sampling of ice cores, most notably the 2001 Mt. Logan summit ice core (187 m to bedrock), for analyses of 34 trace elements (Sr, Cd, Sb, Cs, Ba, Pb, Bi, U, As, Al, S, Ca, Ti, V, Cr, Mn, Fe, Co, Cu, Zn, REE suite) by inductively coupled plasma mass spectrometry (ICP-MS), 8 major ions (Na+, Ca2+, Mg2+, K+, Cl-, SO42-, NO3-, MSA) by ion chromatography (IC), stable water isotopes (δ 18O, δ D, d) and volcanic tephra. The UMaine continuous melter (UMCoM) system is housed in a dedicated clean room with HEPA filtered air. Standard clean room procedures are employed during melting. A Wagenbach-style continuous melter system has been modified to include a pure Nickel melthead that can be easily dismantled for thorough cleaning. The system allows melting of both ice and firn without wicking of the meltwater into unmelted core. Contrary to ice core melter systems in which the meltwater is directly channeled to online instruments for continuous flow analyses, the UMCoM system collects discrete samples for each chemical analysis under ultraclean conditions. Meltwater from the pristine innermost section of the ice core is split between one fraction collector that accumulates ICP-MS samples in acid pre-cleaned polypropylene vials under a class-100 HEPA clean bench, and a second fraction collector that accumulates IC samples. A third fraction collector accumulates isotope and tephra samples from the potentially contaminated outer portion of the core. This method is advantageous because an archive of each sample remains for subsequent analyses (including trace element isotope ratios), and ICP-MS analytes are scanned for longer intervals and in replicate. Method detection limits, calculated from de-ionized water blanks passed through the entire UMCoM system, are below 10% of average Mt. Logan values. A strong correlation (R2>0.9) between Ca and S concentrations measured on different fractions of the same sample by IC and ICP-MS validates sample coregistration. Preliminary analyses of data from the 2001 Mt. Logan summit ice core confirm subannual resolution sampling and annual scale variability of major and trace elements. Accumulation rate models and isotope data suggest that annual resolution will be possible to 1000-2000 y.b.p., with multi-annual to centennial resolution for the remainder of the Holocene and possibly including the last deglaciation. Dust proxy elements, including REEs, strongly co-vary in time-series and reveal concentration ratio fluctuations interpreted as source region changes. Volcanic eruptions are characterized by elevated concentrations of S, SO42-, Cu, Sb, Zn and other trace elements. Concentrations of potential anthropogenic contaminants are also discussed.

Significantly enhanced robustness and electrochemical performance of flexible carbon nanotube-based supercapacitors by electrodepositing polypyrrole

NASA Astrophysics Data System (ADS)

Chen, Yanli; Du, Lianhuan; Yang, Peihua; Sun, Peng; Yu, Xiang; Mai, Wenjie

2015-08-01

Here, we report robust, flexible CNT-based supercapacitor (SC) electrodes fabricated by electrodepositing polypyrrole (PPy) on freestanding vacuum-filtered CNT film. These electrodes demonstrate significantly improved mechanical properties (with the ultimate tensile strength of 16 MPa), and greatly enhanced electrochemical performance (5.6 times larger areal capacitance). The major drawback of conductive polymer electrodes is the fast capacitance decay caused by structural breakdown, which decreases cycling stability but this is not observed in our case. All-solid-state SCs assembled with the robust CNT/PPy electrodes exhibit excellent flexibility, long lifetime (95% capacitance retention after 10,000 cycles) and high electrochemical performance (a total device volumetric capacitance of 4.9 F/cm3). Moreover, a flexible SC pack is demonstrated to light up 53 LEDs or drive a digital watch, indicating the broad potential application of our SCs for portable/wearable electronics.

Effects of ceiling-mounted HEPA-UV air filters on airborne bacteria concentrations in an indoor therapy pool building.

PubMed

Kujundzic, Elmira; Zander, David A; Hernandez, Mark; Angenent, Largus T; Henderson, David E; Miller, Shelly L

2005-02-01

The purpose of this study was to assess the effectiveness of a new generation of high-volume, ceiling-mounted high-efficiency particulate air (HEPA)-ultraviolet (UV) air filters (HUVAFs) for their ability to remove or inactivate bacterial aerosol. In an environmentally controlled full-scale laboratory chamber (87 m3), and an indoor therapy pool building, the mitigation ability of air filters was assessed by comparing concentrations of total bacteria, culturable bacteria, and airborne endotoxin with and without the air filters operating under otherwise similar conditions. Controlled chamber tests with pure cultures of aerosolized Mycobacterium parafortuitum cells showed that the HUVAF unit tested provided an equivalent air-exchange rate of 11 hr(-1). Using this equivalent air-exchange rate as a design basis, three HUVAFs were installed in an indoor therapy pool building for bioaerosol mitigation, and their effectiveness was studied over a 2-year period. The HUVAFs reduced concentrations of culturable bacteria by 69 and 80% during monitoring periods executed in respective years. The HUVAFs reduced concentrations of total bacteria by 12 and 76% during the same monitoring period, respectively. Airborne endotoxin concentrations were not affected by the HUVAF operation.

Monitoring in situ in real time of resin infusion for thermoset composite structures

NASA Astrophysics Data System (ADS)

Faci, A.; Wang, P.; Cochrane, C.; Koncar, V.

2017-10-01

The presented work investigates changes in electrical resistance of embedded sensory yarns as a method to monitor the resin flow front position and curing degree of resin during manufacturing of composite structures by vacuum infusion technology. The sensor concept is based on Piezo-resistive sensors integrated to the flax fabric, having almost identical propriety and dimensions as the flax threads used for the production of reinforcements. In the first time sensors have been characterized and first measures of the resin infusion have been realized in order to demonstrate the feasibility of the proposed approach. Then, the measures in real time were realized with fibrous sensors added to the flax fabric (green composites) to monitor the flow front of resin. A large amount of data recorded, filtered, examined, analysed and processed in order to understand and to optimize the infusion and polymerization process.

Enhanced field electron emission from aligned diamond-like carbon nanorod arrays prepared by reactive ion beam etching

NASA Astrophysics Data System (ADS)

Zhao, Yong; Qin, Shi-Qiao; Zhang, Xue-Ao; Chang, Sheng-Li; Li, Hui-Hui; Yuan, Ji-Ren

2016-05-01

Homogeneous diamond-like carbon (DLC) films were deposited on Si supports by a pulsed filtered cathodic vacuum arc deposition system. Using DLC films masked by Ni nanoparticles as precursors, highly aligned diamond-like carbon nanorod (DLCNR) arrays were fabricated by the etching of inductively coupled radio frequency oxygen plasma. The as-prepared DLCNR arrays exhibit excellent field emission properties with a low turn-on field of 2.005 V μm-1 and a threshold field of 4.312 V μm-1, respectively. Raman spectroscopy and x-ray photoelectron spectroscopy were employed to determine the chemical bonding structural change of DLC films before and after etching. It is confirmed that DLC films have good connection with Si supports via the formation of the SiC phase, and larger conductive sp2 domains are formed in the as-etched DLC films, which play essential roles in the enhanced field emission properties for DLCNR arrays.

Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films

NASA Astrophysics Data System (ADS)

Chen, Hongpeng; Tang, Nan; Chen, Min; Chen, Dihu

2016-03-01

Carbon plasma nanocoatings with controlled fraction of sp3-C bonding were deposited on TiO2 nanorod arrays (TNAs) by DC magnetic-filtered cathodic vacuum arc deposition (FCVAD). The cytocompatibility of TNA/carbon nanocomposites was systematically investigated. Human umbilical vein endothelial cells (HUVECs) were cultured on the nanocomposites for 4, 24, and 72 h in vitro. It was found that plasma-treated TNAs exhibited excellent cell viability as compared to the untreated. Importantly, our results show that cellular responses positively correlate with the sp3-C content. The cells cultured on high sp3-C-contented substrates exhibit better attachment, shape configuration, and proliferation. These findings indicate that the nanocomposites with high sp3-C content possessed superior cytocompatibility. Notably, the nanocomposites drastically reduced platelet adhesion and activation in our previous studies. Taken together, these findings suggest the TNA/carbon scaffold may serve as a guide for the design of multi-functionality devices that promotes endothelialization and improves hemocompatibility.

Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice.

PubMed

Chen, Tian; Jia, Guang; Wei, Yongjie; Li, Jiucun

2013-11-25

Air pollution is associated with significant adverse health effects including increased cardiovascular morbidity and mortality. However research on the cardiovascular effect of "real-world" exposure to ambient particulate matter (PM) in susceptible animal model is very limited. In this study, we aimed to investigate the association between Beijing ambient particle exposure and the atherosclerosis development in the apolipoprotein E knockout mice (ApoE(-/-) mice). Two parallel exposure chambers were used for whole body exposure among ApoE knockout mice. One of the chambers was supplied with untreated ambient air (PM group) and the other chamber was treated with ambient air filtered by high-efficiency particulate air (HEPA) filter (FA group). Twenty mice were divided into two groups and exposed to ambient PM (n=10 for PM group) or filtered air (n=10 for FA group) for two months from January 18th to March 18th, 2010. During the exposure, the mass concentrations of PM2.5 and PM10 in the two chambers were continuously monitored. Additionally, a receptor source apportionment model of chemical mass balance using 19 organic tracers was applied to determine the contributions of sources on the PM2.5 in terms of natural gas, diesel vehicle, gasoline vehicle, coal burning, vegetable debris, biomass burning and cooking. At the end of the two-month exposure, biomarkers of oxidative stress, inflammation and lipid metabolism in bronchoalveolar lavage fluid (BAL) and blood samples were determined and the plaque area on the aortic endothelium was quantified. In the experiment, the concentrations of PM10 and PM2.5 in PM chamber were 99.45μg/m(3) and 61.0μg/m(3) respectively, while PM2.5 in FA chamber was 17.6μg/m(3). Source apportionment analysis by organic tracers showed that gasoline vehicle (39.9%) and coal burning (24.3%) emission were the two major sources contributing to the mass concentration of PM2.5 in Beijing. Among the ApoE knockout mice, the PM group were significantly higher than the FA group in terms of serum total cholesterol, low-density lipoprotein, tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein as well as TNF-alpha and interleukin-6 in BAL. Also the total antioxidant capacity and oxidized low-density lipoprotein were significantly different between the two groups. In addition, pathological analysis of aortic arch reveals that the plaques area in the PM group increased significantly compared to the FA group. Our results demonstrated that ambient PM exposure could induce considerable oxidative stress and systemic inflammation in ApoE knockout mice and contribute to the progression of atherosclerosis. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Upgraded divertor Thomson scattering system on DIII-D

DOE Office of Scientific and Technical Information (OSTI.GOV)

Glass, F., E-mail: glassf@fusion.gat.com; Carlstrom, T. N.; Du, D.

2016-11-15

A design to extend the unique divertor Thomson scattering system on DIII-D to allow measurements of electron temperature and density in high triangularity plasmas is presented. Access to this region is selectable on a shot-by-shot basis by redirecting the laser beam of the existing divertor Thomson system inboard — beneath the lower floor using a moveable, high-damage threshold, in-vacuum mirror — and then redirecting again vertically. The currently measured divertor region remains available with this mirror retracted. Scattered light is collected from viewchords near the divertor floor using in-vacuum, high temperature optical elements and relayed through the port window, beforemore » being coupled into optical fiber bundles. At higher elevations from the floor, measurements are made by dynamically re-focusing the existing divertor system collection optics. Nd:YAG laser timing, analysis of the scattered light spectrum via polychromators, data acquisition, and calibration are all handled by existing systems or methods of the current multi-pulse Thomson scattering system. Existing filtered polychromators with 7 spectral channels are employed to provide maximum measurement breadth (T{sub e} in the range of 0.5 eV–2 keV, n{sub e} in the range of 5 × 10{sup 18}–1 × 10{sup 21} m{sup 3}) for both low T{sub e} in detachment and high T{sub e} measurement up beyond the separatrix.« less

Concerns in Measurement of Healthy Eating and Physical Activity Standards Implementation

ERIC Educational Resources Information Center

Hohman, Katherine H.; Mantinan, Karah D.

2014-01-01

As part of its 2011 commitment to the Partnership for Healthier America, the YMCA of the USA (Y-USA) pledged that by 2015, 85 percent of its local Y associations with early childhood or afterschool programs would have at least one program site that met 100 percent of the Y-USA's healthy eating and physical activity (HEPA) standards. To inform…

Bio-Defense Now: 56 Suggestions for Immediate Improvements

DTIC Science & Technology

2005-05-01

Air Education and Training Command HVAC Heating, Ventilation and Air Conditioning ICAM Improved Chemical Agent Monitor ICD-9-CM Internal...conditioning ( HVAC ) system capabilities, making a big difference in removal of many BW agents. High Efficiency Particulate Air (HEPA) filters are also...agents. This program has developed biological sensor-activated heating, ventilation, and air conditioning ( HVAC ) control sys- tems, high efficiency

Immunogenicity and safety of concomitant administration of a combined hepatitis A/B vaccine and a quadrivalent meningococcal conjugate vaccine in healthy adults.

PubMed

Alberer, Martin; Burchard, Gerd; Jelinek, Tomas; Reisinger, Emil C; Meyer, Seetha; Forleo-Neto, Eduardo; Dagnew, Alemnew F; Arora, Ashwani Kumar

2015-01-01

This phase 3b randomized, open-label study evaluated the immunogenicity and safety of coadministration of a hepatitis A and/or B vaccine with a quadrivalent oligosaccharide meningococcal CRM197 -conjugate vaccine (MenACWY-CRM), in the context of an accelerated hepatitis A and/or B immunization schedule. A total of 252 healthy adult subjects were randomized to three groups to receive hepatitis A/B only (HepA/B), hepatitis A/B coadministered with MenACWY-CRM (HepA/B+MenACWY-CRM), or MenACWY-CRM only (MenACWY-CRM). Hepatitis A and/or B vaccination was administered in the form of a single booster dose or a primary three-dose series, depending on the hepatitis A and/or B vaccination history of subjects. Antibody responses to hepatitis A/B vaccination were assessed 1 month following the last hepatitis A and/or B dose. Serum bactericidal activity with human complement (hSBA) against meningococcal serogroups A, C, W-135, and Y was assessed 1 month post-MenACWY-CRM vaccination. Safety was monitored throughout the study. At 1 month following the final hepatitis A and/or B vaccination, concomitant administration of hepatitis A/B and MenACWY-CRM was non-inferior to administration of hepatitis A/B alone in terms of geometric mean concentrations of antibodies against the hepatitis A and B antigens. One month post-MenACWY-CRM vaccination, the percentages of subjects achieving hSBA titers ≥8 for serogroups A, C, W-135, and Y in the HepA/B+MenACWY-CRM group (76, 87, 99, and 94%, respectively) were comparable to those in the MenACWY-CRM group (67, 82, 96, and 88%, respectively). The percentages of subjects reporting adverse events (AEs) were similar across study groups and a majority of the reported AEs were mild to moderate in nature. There were no study vaccine-related serious AEs. MenACWY-CRM can be administered concomitantly with a hepatitis A and/or B vaccine in the context of an accelerated hepatitis A and/or B immunization schedule without increasing safety concerns or compromising the immune responses to any of the vaccine antigens. [NCT01453348]. © 2014 International Society of Travel Medicine.

Survey of HEPA filter applications and experience at Department of Energy sites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carbaugh, E.H.

1981-11-01

Results indicated that approximately 58% of the filters surveyed were changed out in the 1977 to 1979 study period and some 18% of all filters were changed out more than once. Most changeouts (60%) were due to the existence of a high pressure drop across the filter, indicative of filter plugging. The next most recurrent reasons for changeout and their percentage changeouts were leak test failure (15%) and preventive maintenance service life limit (12%). An average filter service life was calculated to be 3.0 years with a 2.0-year standard deviation. The labor required for filter changeout was calculated as 1.5more » manhours per filter changed. Filter failures occurred with approximately 12% of all installed filters. Most failures (60%) occurred for unknown reasons and handling or installation damage accounted for an additional 20% of all failures. Media ruptures, filter frame failures and seal failures occurred with approximately equal frequency at 5 to 6% each. Subjective responses to the questionnaire indicate problems are: need for improved acid and moisture resistant filters; filters more readily disposable as radioactive waste; improved personnel training in filter handling and installation; and need for pretreatment of air prior to HEPA filtration.« less

Coordinated modulation of albumin synthesis and mRNA levels in cultured hepatoma cells by hydrocortisone and cyclic AMP analogs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, P.C.; Papaconstantinou, J.

The treatment of Hepa-2 cells, a permanent mouse hepatoma cell line, for 72 h with hydrocortisone (10/sup -6/ M), N/sup 6/,O/sup 2/-dibutyryl cyclic AMP (10/sup -3/ M), or 8-bromo cyclic AMP(10/sup -3/ M) results in a 2-, 3-, or 4-fold increase, respectively, in rates of synthesis and secretion of mouse serum albumin. Simultaneous treatment with hydrocortisone and N/sup 6/,O/sup 2/-dibutyryl cyclic AMP results in a 10-fold stimulation in these parameters, an effect that is significantly more than additive for the two compounds tested. The number of albumin mRNA sequences, determined by hybridization of total cell RNA to albumin complementary DNA,more » was increased in direct proportion to the increases in albumin synthesis in all experiments. The relative rate of albumin synthesis approaches in vivo levels in cells treated simultaneously with hydrocortisone and N/sup 6/,O/sup 2/-dibutyryl cyclic AMP. We propose that these factors may be necessary to maintain the maximal level of differentiated function in the continuous culture of Hepa-2 cells.« less

Effects of ambient ozone on first-year growth and physiology of black cherry (Prunus serotina Ehrh. ) seedlings

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kolb, T.E.; Skelly, J.M.; Steiner, K.C.

1993-06-01

Black cherry seedlings of two open-pollinated families were exposed to ambient ozone in open plots (100%) and plots receiving non-filtered (95%), half-filtered (60%) and full-filtered (40%) concentrations via open-top chambers between early June and late-September 1992 in a replicated field experiment in central Pennsylvania. Seasonal 24-hour ambient ozone concentration averaged 34 ppb with a peak 1-hour concentration of 110 ppb. Foliar symptoms of ozone damage (adaxial stipple) occurred most prominently in open and non-filtered plots and differed between families. Net photosynthetic rate for both families was significantly lower in open and non-filtered plots compared with half- and full-filtered plots onmore » most dates, while ozone concentration had no consistent effect on leaf conductance or dark respiration. Leaf conductance of the ozone sensitive family was significantly greater than the ozone tolerant family on most dates. First-year height and diameter growth were significantly lower in open and non-filtered plots compared with half- and full-filtered plots for both families.« less

14 CFR 135.156 - Flight data recorders: filtered data.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight data recorders: filtered data. 135... Aircraft and Equipment § 135.156 Flight data recorders: filtered data. (a) A flight data signal is filtered... original sensor signal value can be reconstructed from the recorded data. This demonstration requires that...

Exploring the covariates of sport participation for health: an analysis of males and females in England.

PubMed

Downward, Paul; Rasciute, Simona

2015-01-01

Increasing sport participation has been identified as a key vehicle for achieving World Health Organisation guidelines for health-enhancing physical activity (HEPA). A policy challenge is to promote this changed behaviour. Making use of the Active People Survey, a zero-inflated ordered probit model is used to identify the covariates of sport participation for males and females with respect to the related decisions to either participate in sport or not, and to participate at either lower or higher than desired intensity. To inform current policy emphases, the Active Places Survey is also used to examine the causal impact of facility provision on these behaviours. Results indicate that females are more likely to adopt less intensive activity even with time available. Families could place most constraints on females, but also reduce the intensity of male participation. Education has a greater association with higher intensity female activity. The largest effects are identified for sport club membership and facility satisfaction. Both of these affect male intensity of activity most. The main policy challenges facing sport in contributing to HEPA are shown to involve retaining male intensity of participation when ageing and encouraging greater female intensity of participation, with more appropriate matching of facilities.

Limiter Observations during W7-X First Plasmas

DOE PAGES

Wurden, Glen Anthony; Biedermann, C.; Effenberg, F.; ...

2017-04-03

During the first operational phase (referred to as OP1.1) of the new Wendelstein 7-X (W7-X) stellarator, five poloidal graphite limiters were mounted on the inboard side of the vacuum vessel, one in each of the five toroidal modules which form the W7-X vacuum vessel. Each limiter consisted of nine specially shaped graphite tiles, designed to conform to the last closed field line geometry in the bean-shaped section of the standard OP1.1 magnetic field configuration (Sunn Pedersen et al 2015 Nucl. Fusion 55 126001). Here, we observed the limiters with multiple infrared and visible camera systems, as well as filtered photomultipliers.more » Power loads are calculated from infrared (IR) temperature measurements using THEODOR, and heating patterns (dual stripes) compare well with field line mapping and EMC3-EIRENE predictions. While the poloidal symmetry of the heat loads was excellent, the toroidal heating pattern showed up to a factor of 2× variation, with peak heat loads on Limiter 1. The total power intercepted by the limiters was up to ~60% of the input ECRH heating power. Calorimetry using bulk tile heating (measured via post-shot IR thermography) on Limiter 3 showed a difference between short high power discharges, and longer lower power ones, with regards to the fraction of energy deposited on the limiters. Finally, fast heating transients, with frequency >1 kHz were detected, and their visibility was enhanced by the presence of surface coatings which developed on the limiters by the end of the campaign.« less

Air filtration systems and restrictive access conditions improve indoor air quality in clinical units: Penicillium as a general indicator of hospital indoor fungal levels.

PubMed

Araujo, Ricardo; Cabral, João Paulo; Rodrigues, Acácio Gonçalves

2008-03-01

High-efficiency particulate air (HEPA) filters do not completely prevent nosocomial fungal infections. The first aim of this study was to evaluate the impact of different filters and access conditions upon airborne fungi in hospital facilities. Additionally, this study identified fungal indicators of indoor air concentrations. Eighteen rooms and wards equipped with different air filter systems, and access conditions were sampled weekly, during 16 weeks. Tap water samples were simultaneously collected. The overall mean concentration of atmospheric fungi for all wards was 100 colony forming units/m(3). We found a direct proportionality between the levels of the different fungi in the studied atmospheres. Wards with HEPA filters at positive air flow yielded lower fungal levels. Also, the existence of an anteroom and the use of protective clothes were associated to the lowest fungal levels. Principal component analysis showed that penicillia afforded the best separation between wards' air fungal levels. Fungal strains were rarely recovered from tap water samples. In addition to air filtration systems, some access conditions to hospital units, like presence of anteroom and use of protective clothes, may prevent high fungal air load. Penicillia can be used as a general indicator of indoor air fungal levels at Hospital S. João.

Characterization of genetically engineered mouse hepatoma cells with inducible liver functions by overexpression of liver-enriched transcription factors.

PubMed

Yamamoto, Hideaki; Tonello, Jane Marie; Sambuichi, Takanori; Kawabe, Yoshinori; Ito, Akira; Kamihira, Masamichi

2018-01-01

New cell sources for the research and therapy of organ failure could significantly alleviate the shortage of donor livers that are available to patients who suffer from liver disease. Liver carcinoma derived cells, or hepatoma cells, are the ideal cells for developing bioartificial liver systems. Such cancerous liver cells are easy to prepare in large quantities and can be maintained over long periods under standard culture conditions, unlike primary hepatocytes. However, hepatoma cells possess only a fraction of the functions of primary hepatocytes. In a previous study, by transducing cells with liver-enriched transcription factors that could be inducibly overexpressed-hepatocyte nuclear factor (HNF)1α, HNF1β, HNF3β [FOXA2], HNF4α, HNF6, CCAAT/enhancer binding protein (C/EBP)α, C/EBPβ and C/EBPγ-we created mouse hepatoma cells with high liver-specific gene expression called the Hepa/8F5 cell line. In the present study, we performed functional and genetic analyses to characterize the Hepa/8F5 cell line. Further, in three-dimensional cultures, the function of these cells improved significantly compared to parental cells. Ultimately, these cells might become a new resource that can be used in basic and applied hepatic research. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Evidence of Coal-Fly-Ash Toxic Chemical Geoengineering in the Troposphere: Consequences for Public Health.

PubMed

Herndon, J Marvin

2015-08-11

The widespread, intentional and increasingly frequent chemical emplacement in the troposphere has gone unidentified and unremarked in the scientific literature for years. The author presents evidence that toxic coal combustion fly ash is the most likely aerosolized particulate sprayed by tanker-jets for geoengineering, weather-modification and climate-modification purposes and describes some of the multifold consequences on public health. Two methods are employed: (1) Comparison of 8 elements analyzed in rainwater, leached from aerosolized particulates, with corresponding elements leached into water from coal fly ash in published laboratory experiments, and (2) Comparison of 14 elements analyzed in dust collected outdoors on a high-efficiency particulate air (HEPA) filter with corresponding elements analyzed in un-leached coal fly ash material. The results show: (1) the assemblage of elements in rainwater and in the corresponding experimental leachate are essentially identical. At a 99% confidence interval, they have identical means (T-test) and identical variances (F-test); and (2) the assemblage of elements in the HEPA dust and in the corresponding average un-leached coal fly ash are likewise essentially identical. The consequences on public health are profound, including exposure to a variety of toxic heavy metals, radioactive elements, and neurologically-implicated chemically mobile aluminum released by body moisture in situ after inhalation or through transdermal induction.

14 CFR 121.346 - Flight data recorders: filtered data.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight data recorders: filtered data. 121... § 121.346 Flight data recorders: filtered data. (a) A flight data signal is filtered when an original... sensor signal value can be reconstructed from the recorded data. This demonstration requires that: (i...

14 CFR 125.228 - Flight data recorders: filtered data.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Flight data recorders: filtered data. 125... Equipment Requirements § 125.228 Flight data recorders: filtered data. (a) A flight data signal is filtered... original sensor signal value can be reconstructed from the recorded data. This demonstration requires that...

Worldwide Report, Epidemiology, No. 332

DTIC Science & Technology

1983-09-14

Patients Die, by Arne Soderlund ANIMAL DISEASES COLOMBIA New Foot-and-Mouth Foci Detected; Vaccination Campaign Discussed (EL TIEMPO, 23 Jul 83... Vaccination "Therefore it is so important for those who are homosexuals with many partners to go to the public health service and be thoroughly checked even...unnecessary anguish can be avoided." Geo von Krogh regards mass vaccination of all homosexual men against B-hepa- titis as one of the many measures to

Limiter Observations during W7-X First Plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wurden, Glen Anthony; Biedermann, C.; Effenberg, F.

During the first operational phase (referred to as OP1.1) of the new Wendelstein 7-X (W7-X) stellarator, five poloidal graphite limiters were mounted on the inboard side of the vacuum vessel, one in each of the five toroidal modules which form the W7-X vacuum vessel. Each limiter consisted of nine specially shaped graphite tiles, designed to conform to the last closed field line geometry in the bean-shaped section of the standard OP1.1 magnetic field configuration (Sunn Pedersen et al 2015 Nucl. Fusion 55 126001). Here, we observed the limiters with multiple infrared and visible camera systems, as well as filtered photomultipliers.more » Power loads are calculated from infrared (IR) temperature measurements using THEODOR, and heating patterns (dual stripes) compare well with field line mapping and EMC3-EIRENE predictions. While the poloidal symmetry of the heat loads was excellent, the toroidal heating pattern showed up to a factor of 2× variation, with peak heat loads on Limiter 1. The total power intercepted by the limiters was up to ~60% of the input ECRH heating power. Calorimetry using bulk tile heating (measured via post-shot IR thermography) on Limiter 3 showed a difference between short high power discharges, and longer lower power ones, with regards to the fraction of energy deposited on the limiters. Finally, fast heating transients, with frequency >1 kHz were detected, and their visibility was enhanced by the presence of surface coatings which developed on the limiters by the end of the campaign.« less

Self-standing paper based anodes prepared from siliconcarbonitride-MoS2 composite for Li-ion battery applications

NASA Astrophysics Data System (ADS)

David, Lamuel; Singh, Gurpreet

2013-03-01

We study synthesis of free-standing polymer derived SiCN/ MoS2 composite paper anode for Li-ion battery application. This was achieved following a two-step approach: First, polysilazane was interfaced with exfoliated MoS2 nanosheets which upon pyrolysis resulted in SiCN/MoS2 composite. Second, dispersion of SiCN/MoS2 in isopropanol was vacuum filtered resulting in formation of a self-standing composite paper. Physical and chemical characterization of the composite was carried out by use of electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and Thermo-gravimetric analysis (TGA). FT-IR data indicated complete conversion of polysilazane precursor to SiCN ceramic, while electron microscopy confirmed layered structure of the paper. Thermo-gravimetric analysis showed enhanced thermodynamic stability of the composite paper up to 800 °C. Electrochemical analysis of SiCN/MoS2 composite paper anodes showed that Li-ion can reversible intercalate in the voltage range of 0-2.5 V with a first cycle discharge capacity of 770 mAh/g at a current density of 100 mA/g.

Using Divertor Strike Point Splitting to Study Plasma Response and Its Sensitivity to Equilibrium Uncertainties

NASA Astrophysics Data System (ADS)

Teklu, Abraham; Orlov, D. M.; Moyer, R. A.; Bykov, I.; Evans, T. E.; Wu, W.; Trevisan, G. L.; Lyons, B. C.; Abrams, T.; Makowski, M. A.; Lasnier, C. S.; Fenstermacher, M. E.

2017-10-01

Resonant magnetic perturbations (RMPs) from 3D coils have been varied to modify the splitting of the divertor strike points in DIII-D. This splitting is imaged in filtered visible and infrared emission from the divertor to determine the particle and heat flux patterns on the target plates. The observed splitting is compared to vacuum and plasma response modeling in discharges where a subset of the RMP coils were ramped to shift the divertor footprints from dominantly n = 3 to n = 2 pattern. These results will be used to determine if the plasma response model can be validated with the measured splitting. We will also study the sensitivity of the modeled splitting to details of the 2D equilibrium. This RMP ramp technique could be used in ITER to spread out the heat flux while avoiding excessive forces on the RMP coils. Work supported by U.S. DOE under the Science Undergraduate Laboratory Internship (SULI) program and DE-FC02-04ER54698, DE-FG02-07ER54917, DE-FG02-05ER54809 and DE-AC52-07NA27344.

Biochemical Testing of Potentially Hazardous Chemicals for Toxicity Using Mammalian Liver Cell Cultures.

DTIC Science & Technology

1992-04-09

the culture medium. The HEPA-2 mouse cells are known to synthesize and to secrete albumin, alpha - fetoprotein , transferrin, ceruloplasmin and...Parker, C.L. and Kute, T.E. (1981). Immunoprecipitation assay of alpha - fetoprotein synthesis by cultured mouse hepatoma cells treated with estrogens and...Infection and Immunity 34:908-914. Rosebrock, J.A., C.L. Parker and T.E. Kute (1981). Immunoprecipitation assay of alpha - fetoprotein synthesis by cultured

Using the GPS to Collect Trajectory Data for Ejection Seat Design, Validation, and Testing

DTIC Science & Technology

2002-03-01

Performing Organization Report Number AFIT/GE/ENG/02M-27 Sponsoring/Monitoring Agency Name(s) and Address(es) AFRL/HEPA ATT: Mr. John Plaga Q...APPROVED FOR PUBLIC RELEASE; DISTRIBUTION UNLIMITED. i Acknowledgments I would like to start by thanking Mr. John Plaga from the Air...Human Effectiveness division, especially Mr. John Plaga for his contributions during the hardware acquisition and configuration. In addition the

Microbiology of the lower ocean crust - Preliminary results from IODP Expedition 360, Atlantis Bank

NASA Astrophysics Data System (ADS)

Sylvan, J. B.; Edgcomb, V. P.; Burgaud, G.; Klein, F.; Schubotz, F.; Expedition 360 Scientists, I.

2016-12-01

International Ocean Discovery Program (IODP) Expedition 360 represents the first leg of a multi-phase drilling program, SloMo, aimed at investigating the nature of the lower crust and Moho at slow spreading ridges. The goal of Expedition 360 was to recover a representative transect of the lower oceanic crust formed at Atlantis Bank, an oceanic core complex on the SW Indian Ridge. We present here preliminary analysis of microbial communities sampled from Hole U1473A, drilled to 789.7 m below seafloor during Expedition 360. Sub-sampling of core sections was conducted in a newly designed plexiglass enclosure with positive air pressure and HEPA filtered air, providing a clean environment for microbiology sampling aboard the JOIDES Resolution. Adenosine triphosphoate, an indicator of microbial biomass, was quantified above detection in 23 of 66 samples analyzed. We measured exoenzyme activity for alkaline phosphatase (AP), leucine aminopeptidase and arginine aminopeptidase in 16 samples and found AP to be very low but above background for 14 of the samples, with highest activities measured between 10 and 70 m below seafloor (mbsf) and peaks again at 158 and 307 mbsf, while both peptidase enzymes were above detection for only one sample at 715 mbsf. Isolates of fungi obtained from core samples as well as analyses of lipid and DNA biomarkers, and Raman spectra for a few of our rock core samples provide initial insights into microbial communities in the lower oceanic crust. Finally, a new tracer of seawater and drilling mud contamination, perfluoromethyl decaline (PFMD), was tested for the first time and its performance compared with the commonly used tracer perfluoromethylcyclohexane (PMCH). PFMD was run during coring operations for ten samples and was routinely detected in the drilling fluids, usually detected on the outside of uncleaned cores, and rarely above detection on the cleaned outside of cores. It was below detection on the inside of cores, indicating penetration of drill fluids to the interior of whole round drill cores, where we collected our samples, is unlikely.

The pathogenesis of ventilator-associated pneumonia: its relevance to developing effective strategies for prevention.

PubMed

Safdar, Nasia; Crnich, Christopher J; Maki, Dennis G

2005-06-01

Ventilator-associated pneumonia (VAP) is the most common nosocomial infection in the intensive care unit and is associated with major morbidity and attributable mortality. Strategies to prevent VAP are likely to be successful only if based upon a sound understanding of pathogenesis and epidemiology. The major route for acquiring endemic VAP is oropharyngeal colonization by the endogenous flora or by pathogens acquired exogenously from the intensive care unit environment, especially the hands or apparel of health-care workers, contaminated respiratory equipment, hospital water, or air. The stomach represents a potential site of secondary colonization and reservoir of nosocomial Gram-negative bacilli. Endotracheal-tube biofilm formation may play a contributory role in sustaining tracheal colonization and also have an important role in late-onset VAP caused by resistant organisms. Aspiration of microbe-laden oropharyngeal, gastric, or tracheal secretions around the cuffed endotracheal tube into the normally sterile lower respiratory tract results in most cases of endemic VAP. In contrast, epidemic VAP is most often caused by contamination of respiratory therapy equipment, bronchoscopes, medical aerosols, water (eg, Legionella) or air (eg, Aspergillus or the severe acute respiratory syndrome virus). Strategies to eradicate oropharyngeal and/or intestinal microbial colonization, such as with chlorhexidine oral care, prophylactic aerosolization of antimicrobials, selective aerodigestive mucosal antimicrobial decontamination, or the use of sucralfate rather than H(2) antagonists for stress ulcer prophylaxis, and measures to prevent aspiration, such as semirecumbent positioning or continuous subglottic suctioning, have all been shown to reduce the risk of VAP. Measures to prevent epidemic VAP include rigorous disinfection of respiratory equipment and bronchoscopes, and infection-control measures to prevent contamination of medical aerosols. Hospital water should be Legionella-free, and high-risk patients, especially those with prolonged granulocytopenia or organ transplants, should be cared for in hospital units with high-efficiency-particulate-arrestor (HEPA) filtered air. Routine surveillance of VAP, to track endemic VAPs and facilitate early detection of outbreaks, is mandatory.

Verification of sub-grid filtered drag models for gas-particle fluidized beds with immersed cylinder arrays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sarkar, Avik; Sun, Xin; Sundaresan, Sankaran

2014-04-23

The accuracy of coarse-grid multiphase CFD simulations of fluidized beds may be improved via the inclusion of filtered constitutive models. In our previous study (Sarkar et al., Chem. Eng. Sci., 104, 399-412), we developed such a set of filtered drag relationships for beds with immersed arrays of cooling tubes. Verification of these filtered drag models is addressed in this work. Predictions from coarse-grid simulations with the sub-grid filtered corrections are compared against accurate, highly-resolved simulations of full-scale turbulent and bubbling fluidized beds. The filtered drag models offer a computationally efficient yet accurate alternative for obtaining macroscopic predictions, but the spatialmore » resolution of meso-scale clustering heterogeneities is sacrificed.« less

Construction, Testing, and Analysis of Radon Mitigation System

NASA Astrophysics Data System (ADS)

Jardin, Dan; Schnee, Richard; CDMS Collaboration

2011-10-01

The search for dark matter or other rare events such as neutrinoless double-beta decay is difficult in the presence of background radiation such as the alpha and beta emissions from the 222Rn decay chain. In order to reduce the radioactive background from Rn-daughters, an ultra-low radon clean room is being built at Syracuse University. A vacuum-swing adsorption system is used to mitigate the radon. Air flows through one of two tanks filled with charcoal that the radon adsorbs to, allowing the filtered air to pass into the clean room. Computer-controlled valves direct the airflow so that one tank filters the air while the other tank is purged of radon by circulating a small fraction of the cleaned airflow back through the tank at low pressure. The durations, pressures, and flow rates of each stage of building pressure, filtering, releasing pressure, and purging in the tanks are optimized in order to maximize the reduction of radon from the air. Professor.

Multivariate frequency domain analysis of protein dynamics

NASA Astrophysics Data System (ADS)

Matsunaga, Yasuhiro; Fuchigami, Sotaro; Kidera, Akinori

2009-03-01

Multivariate frequency domain analysis (MFDA) is proposed to characterize collective vibrational dynamics of protein obtained by a molecular dynamics (MD) simulation. MFDA performs principal component analysis (PCA) for a bandpass filtered multivariate time series using the multitaper method of spectral estimation. By applying MFDA to MD trajectories of bovine pancreatic trypsin inhibitor, we determined the collective vibrational modes in the frequency domain, which were identified by their vibrational frequencies and eigenvectors. At near zero temperature, the vibrational modes determined by MFDA agreed well with those calculated by normal mode analysis. At 300 K, the vibrational modes exhibited characteristic features that were considerably different from the principal modes of the static distribution given by the standard PCA. The influences of aqueous environments were discussed based on two different sets of vibrational modes, one derived from a MD simulation in water and the other from a simulation in vacuum. Using the varimax rotation, an algorithm of the multivariate statistical analysis, the representative orthogonal set of eigenmodes was determined at each vibrational frequency.

Fabrication of optically transparent chitin nanocomposites

NASA Astrophysics Data System (ADS)

Shams, M. Iftekhar; Ifuku, Shinsuke; Nogi, Masaya; Oku, Takeshi; Yano, Hiroyuki

2011-02-01

This paper demonstrates the preparation of chitin nanofibers from crab shells using a simple mechanical treatment. The nanofibers are small enough to retain the transparency of neat acrylic resin. Possessing hydroxyl and amine/ N-acetyl functionalities, water suspension of chitin nanofibers was vacuum-filtered 9 times faster than cellulose nanofibers to prepare a nanofiber sheet of 90 mm in diameter. This is a prominent advantage of chitin nanofibers over cellulose nanofibers in terms of commercial application. Interestingly, chitin acrylic resin films exhibited much higher transparency than cellulose acrylic resin films owing to the close affinity between less hydrophilic chitin and hydrophobic resin. Furthermore, the incorporation of chitin nanofibers contributes to the significant improvement of the thermal expansion and mechanical properties of the neat acrylic resin. The properties of high light transmittance and low thermal expansion make chitin nanocomposites promising candidates for the substrate in a continuous roll-to-roll process in the manufacturing of various optoelectronic devices such as flat panel displays, bendable displays, and solar cells.

Home interventions are effective at decreasing indoor nitrogen dioxide concentrations

PubMed Central

Paulin, L. M.; Diette, G. B.; Scott, M.; McCormack, M. C.; Matsui, E. C.; Curtin-Brosnan, J.; Williams, D. L.; Kidd-Taylor, A.; Shea, M.; Breysse, P. N.; Hansel, N. N.

2016-01-01

Nitrogen dioxide (NO2), a by-product of combustion produced by indoor gas appliances such as cooking stoves, is associated with respiratory symptoms in those with obstructive airways disease. We conducted a three-armed randomized trial to evaluate the efficacy of interventions aimed at reducing indoor NO2 concentrations in homes with unvented gas stoves: (i) replacement of existing gas stove with electric stove; (ii) installation of ventilation hood over existing gas stove; and (iii) placement of air purifiers with high-efficiency particulate air (HEPA) and carbon filters. Home inspection and NO2 monitoring were conducted at 1 week pre-intervention and at 1 week and 3 months post-intervention. Stove replacement resulted in a 51% and 42% decrease in median NO2 concentration at 3 months of follow-up in the kitchen and bedroom, respectively (P = 0.01, P = 0.01); air purifier placement resulted in an immediate decrease in median NO2 concentration in the kitchen (27%, P < 0.01) and bedroom (22%, P = 0.02), but at 3 months, a significant reduction was seen only in the kitchen (20%, P = 0.05). NO2 concentrations in the kitchen and bedroom did not significantly change following ventilation hood installation. Replacing unvented gas stoves with electric stoves or placement of air purifiers with HEPA and carbon filters can decrease indoor NO2 concentrations in urban homes. PMID:24329966

The effect of bicarbonate on menadione-induced redox cycling and cytotoxicity: potential involvement of the carbonate radical.

PubMed

Aljuhani, Naif; Michail, Karim; Karapetyan, Zubeida; Siraki, Arno G

2013-10-01

We have investigated the effect of NaHCO3 on menadione redox cycling and cytotoxicity. A cell-free system utilized menadione and ascorbic acid to catalyze a redox cycle, and we utilized murine hepatoma (Hepa 1c1c7) cells for in vitro experiments. Experiments were performed using low (2 mmol/L) and physiological (25 mmol/L) levels of NaHCO3 in buffer equilibrated to physiological pH. Using oximetry, ascorbic acid oxidation, and ascorbyl radical detection, we found that menadione redox cycling was enhanced by NaHCO3. Furthermore, Hepa 1c1c7 cells treated with menadione demonstrated cytotoxicity that was significantly increased with physiological concentrations of NaHCO3 in the media, compared with low levels of NaHCO3. Interestingly, the inhibition of superoxide dismutase (SOD) with 2 different metal chelators was associated with a protective effect against menadione cytotoxicity. Using isolated protein, we found a significant increase in protein carbonyls with menadione-ascorbate-SOD with physiological NaHCO3 levels; low NaHCO3 or SOD-free reactions produced lower levels of protein carbonyls. In conclusion, these findings suggest that the hydrogen peroxide generated by menadione redox cycling together with NaHCO3-CO2 are potential substrates for SOD peroxidase activity that can lead to carbonate-radical-enhanced cytotoxicity. These findings demonstrate the importance of NaHCO3 in menadione redox cycling and cytotoxicity.

Nutrient Considerations for Plants Grown Under Space Flight Conditions

NASA Technical Reports Server (NTRS)

Levine, Howard G.; Krikorian, Abraham D.

2006-01-01

We present here results on the analysis of 100 mL medium samples extracted from sterilized foam (Smithers-Oasis, Kent OH) used to support the growth of both dicotyledonous (Haplopappus gracilis, n=75) and monocotyledonous (Hemerocallis cv Autumn Blaze, n=25) aseptic plants in NASA's Plant Growth Unit (PGU) during the 5-day CHROMEX-01 Space Shuttle flight (March 1989, STS-29). At recovery, the medium remaining within each of the five floral foam blocks (for both the space flight and ground control experiments) was extracted under vacuum, filtered and subjected to elemental analyses. Concentration levels of some elements remained the same, while some decreased and others increased. A unique aspect of this experiment was that all plants were either aseptic tissue culture generated plantlets or sterile seedling clones, and the design of the PGU facilitated the maintenance of asepsis throughout the mission (confirmed by postflight microbial sampling). This permitted the elimination of microbial considerations in the interpretation of the data. The significance of these findings for growing plants in altered gravity environments are discussed.

Preparation and analysis of particulate metal deposits

NASA Technical Reports Server (NTRS)

Poppa, H.; Moorhead, D.; Heinemann, K.

1985-01-01

Small particles and clusters of palladium were grown by deposition from the vapor phase under ultrahigh vacuum conditions. Amorphous and crystalline support films of Al2O3 and ultrathin amorphous carbon films were used as substrate materials. The growth of the metal deposit was monitored in situ by scanning transmission diffraction of energy-filtered 100 kV electrons and high resolution transmission electron microscopy (TEM) analysis was performed in a separate instrument. It was established by in situ TEM, however, that the transfer of specimens in this case did not unduly affect the size and distribution of deposit particles. It was found that the cleanness, stoichiometry, crystallinity and structural perfection of the support surface play an essential role in determining the crystalline perfection and structure of the particles. The smallest palladium clusters reproducibly prepared contained not more than six atoms but size determinations below 1 nm average particle diameter are very problematic with conventional TEM. Palladium particles grown on carbon supports feature an impurity-stabilized mosaic structure.

Interfacial Studies of Whisker and Coated Fiber Reinforced Ceramic Matrix Composites

DTIC Science & Technology

1990-05-31

well as BN coated small diameter (ɘ.7jtm) ARCO whiskers. Tha carbon coated TWS- 400C whiskers were received as-coated from Textron, Inc., Lowell, MA...under negative pressure by means of a Nilfisk filtering system equipped with a Hepa filter. With the health hazards of small whiskers being of utmost...Both of these platelet types were analyzed in the scanning Auger multiprobe (SAM) and found to be very close to stoichiometric SiC with a small amount

DOE Office of Scientific and Technical Information (OSTI.GOV)

Iyer, Ajai, E-mail: ajai.iyer@aalto.fi; Etula, Jarkko; Liu, Xuwen

Single walled carbon nanotube networks (SWCNTNs) were coated by tetrahedral amorphous carbon (ta-C) to improve the mechanical wear properties of the composite film. The ta-C deposition was performed by using pulsed filtered cathodic vacuum arc method resulting in the generation of C+ ions in the energy range of 40–60 eV which coalesce to form a ta-C film. The primary disadvantage of this process is a significant increase in the electrical resistance of the SWCNTN post coating. The increase in the SWCNTN resistance is attributed primarily to the intrinsic stress of the ta-C coating which affects the inter-bundle junction resistance between themore » SWCNTN bundles. E-beam evaporated carbon was deposited on the SWCNTNs prior to the ta-C deposition in order to protect the SWCNTN from the intrinsic stress of the ta-C film. The causes of changes in electrical resistance and the effect of evaporated carbon thickness on the changes in electrical resistance and mechanical wear properties have been studied.« less

Measurement of the attenuation length of argon scintillation light in the ArDM LAr TPC

NASA Astrophysics Data System (ADS)

Calvo, J.; Cantini, C.; Crivelli, P.; Daniel, M.; Di Luise, S.; Gendotti, A.; Horikawa, S.; Molina-Bueno, L.; Montes, B.; Mu, W.; Murphy, S.; Natterer, G.; Nguyen, K.; Periale, L.; Quan, Y.; Radics, B.; Regenfus, C.; Romero, L.; Rubbia, A.; Santorelli, R.; Sergiampietri, F.; Viant, T.; Wu, S.

2018-01-01

We report on a measurement of the attenuation length for the scintillation light in the tonne size liquid argon target of the ArDM dark matter experiment. The data was recorded in the first underground operation of the experiment in single-phase operational mode. The results were achieved by comparing the light yield spectra from 39Ar and 83mKr to a description of the ArDM setup with a model of full light ray tracing. A relatively low value close to 0.5 m was found for the attenuation length of the liquid argon bulk to its own scintillation light. We interpret this result as a presence of optically active impurities in the liquid argon which are not filtered by the installed purification systems. We also present analyses of the argon gas employed for the filling and discuss cross sections in the vacuum ultraviolet of various molecules in respect to purity requirements in the context of large liquid argon installations.

Ultrahigh performance heat spreader based on gas-liquid exfoliation boron nitride nanosheets

NASA Astrophysics Data System (ADS)

Ding, Jiheng; Zhao, Hongran; Wang, Qiaolei; Peng, Wanjun; Yu, Haibin

2017-11-01

Due to their high thermal conductivity and insulation performance, boron nitride nanosheets (BNNS) have great promise to fabricate thermal management equipment for application in power electronics. The liquid-phase exfoliation route has been regarded as the most commonly used approach to produce single and few-layered BNNS for many research fields. However, this process takes a long time, and the production yield is extremely low. In this work, an efficient technique to obtain few-layered (mostly < 5 layers), high-yield (˜33%), and plane-defect-free BNNS by the combination of liquid N2 (L-N2) gasification and liquid exfoliation was developed. The as-obtained BNNS suspensions could be vacuum filtered to make a thermal conductive film named a BNNS heat spreader which possessed a superior thermal conductivity of 61.2 W m-1 K-1 at room temperature. In addition, we also proved that the thermal conductivity of the BNNS heat spreader increased with the increase of density, creating an approach for fine tuning the thermal property of this heat spreader.

Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory; extraction of nitroaromatic compounds from water by polystyrene divinylbenzene cartridge and determination by high-performance liquid chromatography

USGS Publications Warehouse

Lindley, C.E.; Burkhardt, M.R.; DeRusseau, S.N.

1994-01-01

Organic explosives are determined in samples of ground water and surface water with emphasis on identifying and quantifying trinitrotoluene (TNT) metabolites. Water samples are filtered to remove suspended particulate material and passed through a polystyrene divinylbenzene-packed cartridge by a vacuum-extraction system. The target analytes subsequently are eluted with acetonitrile. A high-performance liquid chromatograph (HPLC) equipped with a photodiode-array detector is used for sample analysis. Analytes are separated on an octadecylsilane column using a methanol, water, and acetonitrile gradient elution. The compounds 2,4- and 2,6-dinitrotoluene are separated through an independent, isocratic elution. Method detection limits, on the basis of a 1-liter sample size, range from 0.11 to 0.32 microgram per liter. Recoveries averaged from 71 to 101 percent for 13 analytes in one set of HPLC-grade water fortified at about 1 microgram per liter. The method is limited to use by analysts experienced in handling explosive materials. (USGS)

National, State, and Selected Local Area Vaccination Coverage Among Children Aged 19-35 Months - United States, 2014.

PubMed

Hill, Holly A; Elam-Evans, Laurie D; Yankey, David; Singleton, James A; Kolasa, Maureen

2015-08-28

The reduction in morbidity and mortality associated with vaccine-preventable diseases in the United States has been described as one of the 10 greatest public health achievements of the first decade of the 21st century. A recent analysis concluded that routine childhood vaccination will prevent 322 million cases of disease and about 732,000 early deaths among children born during 1994-2013, for a net societal cost savings of $1.38 trillion. The National Immunization Survey (NIS) has monitored vaccination coverage among U.S. children aged 19-35 months since 1994. This report presents national, regional, state, and selected local area vaccination coverage estimates for children born from January 2011 through May 2013, based on data from the 2014 NIS. For most vaccinations, there was no significant change in coverage between 2013 and 2014. The exception was hepatitis A vaccine (HepA), for which increases were observed in coverage with both ≥1 and ≥2 doses. As in previous years, <1% of children received no vaccinations. National coverage estimates indicate that the Healthy People 2020 target* of 90% was met for ≥3 doses of poliovirus vaccine (93.3%), ≥1 dose of measles, mumps, and rubella vaccine (MMR) (91.5%), ≥3 doses of hepatitis B vaccine (HepB) (91.6%), and ≥1 dose of varicella vaccine (91.0%). Coverage was below target for ≥4 doses of diphtheria, tetanus, and acellular pertussis vaccine (DTaP), the full series of Haemophilus influenzae type b (Hib) vaccine, hepatitis B (HepB) birth dose,† ≥4 doses pneumococcal conjugate vaccine (PCV), ≥2 doses of HepA, the full series of rotavirus vaccine, and the combined vaccine series.§ Examination of coverage by child's race/ethnicity revealed lower estimated coverage among non-Hispanic black children compared with non-Hispanic white children for several vaccinations, including DTaP, the full series of Hib, PCV, rotavirus vaccine, and the combined series. Children from households classified as below the federal poverty level had lower estimated coverage for almost all of the vaccinations assessed, compared with children living at or above the poverty level. Significant variation in coverage by state¶ was observed for several vaccinations, including HepB birth dose, HepA, and rotavirus. High vaccination coverage must be maintained across geographic and sociodemographic groups if progress in reducing the impact of vaccine-preventable diseases is to be sustained.

Perception of Filtered Speech by Children with Developmental Dyslexia and Children with Specific Language Impairments

PubMed Central

Goswami, Usha; Cumming, Ruth; Chait, Maria; Huss, Martina; Mead, Natasha; Wilson, Angela M.; Barnes, Lisa; Fosker, Tim

2016-01-01

Here we use two filtered speech tasks to investigate children’s processing of slow (<4 Hz) versus faster (∼33 Hz) temporal modulations in speech. We compare groups of children with either developmental dyslexia (Experiment 1) or speech and language impairments (SLIs, Experiment 2) to groups of typically-developing (TD) children age-matched to each disorder group. Ten nursery rhymes were filtered so that their modulation frequencies were either low-pass filtered (<4 Hz) or band-pass filtered (22 – 40 Hz). Recognition of the filtered nursery rhymes was tested in a picture recognition multiple choice paradigm. Children with dyslexia aged 10 years showed equivalent recognition overall to TD controls for both the low-pass and band-pass filtered stimuli, but showed significantly impaired acoustic learning during the experiment from low-pass filtered targets. Children with oral SLIs aged 9 years showed significantly poorer recognition of band pass filtered targets compared to their TD controls, and showed comparable acoustic learning effects to TD children during the experiment. The SLI samples were also divided into children with and without phonological difficulties. The children with both SLI and phonological difficulties were impaired in recognizing both kinds of filtered speech. These data are suggestive of impaired temporal sampling of the speech signal at different modulation rates by children with different kinds of developmental language disorder. Both SLI and dyslexic samples showed impaired discrimination of amplitude rise times. Implications of these findings for a temporal sampling framework for understanding developmental language disorders are discussed. PMID:27303348

Evidence of Coal-Fly-Ash Toxic Chemical Geoengineering in the Troposphere: Consequences for Public Health

PubMed Central

Herndon, J. Marvin

2015-01-01

The widespread, intentional and increasingly frequent chemical emplacement in the troposphere has gone unidentified and unremarked in the scientific literature for years. The author presents evidence that toxic coal combustion fly ash is the most likely aerosolized particulate sprayed by tanker-jets for geoengineering, weather-modification and climate-modification purposes and describes some of the multifold consequences on public health. Two methods are employed: (1) Comparison of 8 elements analyzed in rainwater, leached from aerosolized particulates, with corresponding elements leached into water from coal fly ash in published laboratory experiments, and (2) Comparison of 14 elements analyzed in dust collected outdoors on a high-efficiency particulate air (HEPA) filter with corresponding elements analyzed in un-leached coal fly ash material. The results show: (1) the assemblage of elements in rainwater and in the corresponding experimental leachate are essentially identical. At a 99% confidence interval, they have identical means (T-test) and identical variances (F-test); and (2) the assemblage of elements in the HEPA dust and in the corresponding average un-leached coal fly ash are likewise essentially identical. The consequences on public health are profound, including exposure to a variety of toxic heavy metals, radioactive elements, and neurologically-implicated chemically mobile aluminum released by body moisture in situ after inhalation or through transdermal induction. PMID:26270671

Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: a potential novel strategy to enhance zinc absorption by ZIP4 targeting.

PubMed

Hashimoto, Ayako; Ohkura, Katsuma; Takahashi, Masakazu; Kizu, Kumiko; Narita, Hiroshi; Enomoto, Shuichi; Miyamae, Yusaku; Masuda, Seiji; Nagao, Masaya; Irie, Kazuhiro; Ohigashi, Hajime; Andrews, Glen K; Kambe, Taiho

2015-12-01

Dietary zinc deficiency puts human health at risk, so we explored strategies for enhancing zinc absorption. In the small intestine, the zinc transporter ZIP4 functions as an essential component of zinc absorption. Overexpression of ZIP4 protein increases zinc uptake and thereby cellular zinc levels, suggesting that food components with the ability to increase ZIP4 could potentially enhance zinc absorption via the intestine. In the present study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components that can increase the abundance of ZIP4. Using this ZIP4-targeting strategy, two such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin-Darby canine kidney cells and, moreover, two apically localized mZip4 acrodermatitis enteropathica mutants. Soybean components were purified from one extract and soyasaponin Bb was identified as an active component that increased both mZip4 protein abundance and zinc levels in Hepa cells. Finally, we confirmed that soyasaponin Bb is capable of enhancing cell surface endogenous human ZIP4 in human cells. Our results suggest that ZIP4 targeting may represent a new strategy to improve zinc absorption in humans. © 2015 Authors; published by Portland Press Limited.

Air filtration in the free molecular flow regime: a review of high-efficiency particulate air filters based on carbon nanotubes.

PubMed

Li, Peng; Wang, Chunya; Zhang, Yingying; Wei, Fei

2014-11-01

Air filtration in the free molecular flow (FMF) regime is important and challenging because a higher filtration efficiency and lower pressure drop are obtained when the fiber diameter is smaller than the gas mean free path in the FMF regime. In previous studies, FMF conditions have been obtained by increasing the gas mean free path through reducing the pressure and increasing the temperature. In the case of carbon nanotubes (CNTs) with nanoscale diameters, it is possible to filtrate in the FMF regime under normal conditions. This paper reviews recent progress in theoretical and experimental studies of air filtration in the FMF regime. Typical structure models of high-efficiency particulate (HEPA) air filters based on CNTs are introduced. The pressure drop in air filters operated in the FMF regime is less than that predicted by the conventional air filtration theory. The thinnest HEPA filters fabricated from single-walled CNT films have an extremely low pressure drop. CNT air filters with a gradient nanostructure are shown to give a much better filtration performance in dynamic filtration. CNT air filters with a hierarchical structure and an agglomerated CNT fluidized bed air filter are also introduced. Finally, the challenges and opportunities for the application of CNTs in air filtration are discussed. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Advanced Filter Technology For Nuclear Thermal Propulsion

NASA Technical Reports Server (NTRS)

Castillon, Erick

2015-01-01

The Scrubber System focuses on using HEPA filters and carbon filtration to purify the exhaust of a Nuclear Thermal Propulsion engine of its aerosols and radioactive particles; however, new technology may lend itself to alternate filtration options, which may lead to reduction in cost while at the same time have the same filtering, if not greater, filtering capabilities, as its predecessors. Extensive research on various types of filtration methods was conducted with only four showing real promise: ionization, cyclonic separation, classic filtration, and host molecules. With the four methods defined, more research was needed to find the devices suitable for each method. Each filtration option was matched with a device: cyclonic separators for the method of the same name, electrostatic separators for ionization, HEGA filters, and carcerands for the host molecule method. Through many hours of research, the best alternative for aerosol filtration was determined to be the electrostatic precipitator because of its high durability against flow rate and its ability to cleanse up to 99.99% of contaminants as small as 0.001 micron. Carcerands, which are the only alternative to filtering radioactive particles, were found to be non-existent commercially because of their status as a "work in progress" at research institutions. Nevertheless, the conclusions after the research were that HEPA filters is recommended as the best option for filtering aerosols and carbon filtration is best for filtering radioactive particles.

Survival of microorganisms in space protected by meteorite material: results of the experiment 'EXOBIOLOGIE' of the PERSEUS mission.

PubMed

Rettberg, P; Eschweiler, U; Strauch, K; Reitz, G; Horneck, G; Wanke, H; Brack, A; Barbier, B

2002-01-01

During the early evolution of life on Earth, before the formation of a protective ozone layer in the atmosphere, high intensities of solar UV radiation of short wavelengths could reach the surface of the Earth. Today the full spectrum of solar UV radiation is only experienced in space, where other important space parameters influence survival and genetic stability additionally, like vacuum, cosmic radiation, temperature extremes, microgravity. To reach a better understanding of the processes leading to the origin, evolution and distribution of life we have performed space experiments with microorganisms. The ability of resistant life forms like bacterial spores to survive high doses of extraterrestrial solar UV alone or in combination with other space parameters, e.g. vacuum, was investigated. Extraterrestrial solar UV was found to have a thousand times higher biological effectiveness than UV radiation filtered by stratospheric ozone concentrations found today on Earth. The protective effects of anorganic substances like artificial or real meteorites were determined on the MIR station. In the experiment EXOBIOLOGIE of the French PERSEUS mission (1999) it was found that very thin layers of anorganic material did not protect spores against the deleterious effects of energy-rich UV radiation in space to the expected amount, but that layers of UV radiation inactivated spores serve as a UV-shield by themselves, so that a hypothetical interplanetary transfer of life by the transport of microorganisms inside rocks through the solar system cannot be excluded, but requires the shielding of a substantial mass of anorganic substances. c2002 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Global HRSC Image Mosaics of Mars: Dodging for High-Pass Filtering, Combined with Low-Pass-Filtered OMEGA Mosaics

NASA Astrophysics Data System (ADS)

McGuire, P. C.; Walter, S. H. G.; van Gasselt, S.; Dumke, A.; Dunker, T.; Gross, C.; Michael, G.; Wendt, L.; Audouard, J.; Ody, A.; Poulet, F.

2014-07-01

We discuss our approach towards automatically mosaicking hundreds of the HRSC panchromatic or RGB images together. Our best results consist of adding a high-pass-filtered HRSC mosaic to a low-pass-filtered OMEGA global mosaic.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carter, E.E.; Welty, B.D.

Molten wax shows considerable promise as a fixative and dust control agent in demolition of radioactively contaminated facilities. Sticky molten wax, modified with special surfactants and wetting agents, is capable of not only coating materials but also penetrating into friable or dusty materials and making them incapable of becoming airborne during demolition. Wax also shows significant promise for stabilization of waste residuals that may be contained in buildings undergoing demolition. Some of the building materials that have been tested to date include concrete, wood, sheet rock, fiber insulation, lime, rock, and paper. Protective clothing, clay, sand, sulfur, and bentonite claymore » have been tested as surrogates for certain waste materials that may be encountered during building demolition. The paper describes several potential applications of molten wax for dust control in demolition of radioactive contaminated facilities. As a case-study, this paper describes a research test performed for a pipeline closure project being completed by the Idaho Cleanup Project at the Idaho National Laboratory. The project plans to excavate and remove a section of buried Duriron drain piping containing highly radioactive and friable and 'flighty' waste residuals. A full-scale pipeline mockup containing simulated waste was buried in sand to simulate the direct buried subsurface condition of the subject piping. The pipeline was pre-heated by drawing hot air through the line with a HEPA vacuum blower unit. Molten wax was pumped into the line and allowed to cool. The line was then broken apart in various places to evaluate the permeation performance of the wax. The wax fully permeated all the surrogate materials rendering them non-friable with a consistency similar to modeling clay. Based on the performance during the mockup, it is anticipated that the wax will be highly effective in controlling the spread of radiological contamination during pipe demolition activities. (authors)« less

Viral Penetration of High Efficiency Particulate Air (HEPA) Filters

DTIC Science & Technology

2007-02-01

ml. For aerosol studies the MS2 coli phage was either diluted in sterile distilled water or 0.5% tryptone to a concentration ~108 PFU/mL. Collison...increased to 38%; an increase of 30.5% over MS2 suspended in water . The mass curve is not complete, and thus the fraction of particles in the 100–300 nm...a decrease of 14% over what is observed for MS2 suspended in water . The overall number of particles generated by MS2 + 0.5% tryptone and MS2 in

Acute Oral Toxicity of Nitroguanidine in Mice

DTIC Science & Technology

1988-04-01

report, tbe iav tl at loa adhered to the "Guide for t e Care and U or Laboratory Animals," promulrat d by tbe Committee on Rev’ ion of tlte Guid for...microscopic examination. These areas did not respond t o special bacterial stains and may have been due to mouse hepa t i tis virus . Mic r oscopic...iiisfil ’iy noin«’ Iwp.ititio virus . An mal ID*! 84C003<»7 P.it.holooy Af-ci’ssion »•■ 30)17. Slide 19A! Eye

7. VIEW LOOKING WEST DOWN CENTRAL AVENUE AT THE INTERSECTION ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. VIEW LOOKING WEST DOWN CENTRAL AVENUE AT THE INTERSECTION WITH SEVENTH STREET. THE PLANT WAS BUILT ON THE SITE WITH FOUR SEPARATE PRODUCTION AREAS, AND AN ADMINISTRATION AND SUPPORT AREA. THIS PHOTOGRAPH SHOWS THE EASTERN EDGE OF THE CORE ADMINSTRATION AND SUPPORT AREA, BUILT IN THE EARLY 1950S. IN THE LEFT FOREGROUND OF THE PHOTOGRAPH IS BUILDING 442, USED TO TEST ALL HEPA FILTERS ON SITE. - Rocky Flats Plant, Bounded by Indiana Street & Routes 93, 128 & 72, Golden, Jefferson County, CO

Tank 241-Z-361 Sludge Retrieval and Treatment Alternatives

DOE Office of Scientific and Technical Information (OSTI.GOV)

HAMPTON, B.K.

2000-05-24

The Plutonium Finishing Plant (PFP) Tank 241-Z-361 (Z-361) contains legacy sludge resulting from waste discharges from past missions at PFP. A sketch of the tank is shown in Figure 1. In this view various risers and penetrations are shown along with the sludge level depicted by the horizontal line halfway up the tank, and the ground level depicted by the horizontal line above the tank. The HEPA filter installed for breathing is also shown on one of the risers.

40 CFR 141.73 - Filtration.

Code of Federal Regulations, 2014 CFR

2014-07-01

... turbidity level of representative samples of a system's filtered water must be less than or equal to 0.5 NTU....74 (a)(1) and (c)(1). (2) The turbidity level of representative samples of a system's filtered water... filtration, the turbidity level of representative samples of a system's filtered water must be less than or...

Magnetic tunnel junctions utilizing diamond-like carbon tunnel barriers

NASA Astrophysics Data System (ADS)

Cadieu, F. J.; Chen, Li; Li, Biao

2002-05-01

We have devised a method whereby thin particulate-free diamond-like carbon films can be made with good adhesion onto even room-temperature substrates. The method employs a filtered ionized carbon beam created by the vacuum impact of a high-energy, approximately 1 J per pulse, 248 nm excimer laser onto a carbon target. The resultant deposition beam can be steered and deflected by magnetic and electric fields to paint a specific substrate area. An important aspect of this deposition method is that the resultant films are particulate free and formed only as the result of atomic species impact. The vast majority of magnetic tunnel junctions utilizing thin metallic magnetic films have employed a thin oxidized layer of aluminum to form the tunnel barrier. This has presented reproducibility problems because the indicated optimal barrier thickness is only approximately 13 Å thick. Magnetic tunnel junctions utilizing Co and permalloy films made by evaporation and sputtering have been fabricated with an intervening diamond-like carbon tunnel barrier. The diamond-like carbon thickness profile has been tapered so that seven junctions with different barrier thickness can be formed at once. Magnetoresistive (MR) measurements made between successive permalloy strip ends include contributions from two junctions and from the permalloy and Co strips that act as current leads to the junctions. Magnetic tunnel junctions with thicker carbon barriers exhibit MR effects that are dominated by that of the permalloy strips. Since these tunnel barriers are formed without the need for oxygen, complete tunnel junctions can be formed with all high-vacuum processing.

Synthesis of Ti-doped DLC film on SS304 steels by Filtered Cathodic Vacuum Arc (FCVA) technique for tribological improvement

NASA Astrophysics Data System (ADS)

Bootkul, D.; Saenphinit, N.; Supsermpol, B.; Aramwit, C.; Intarasiri, S.

2014-08-01

Currently, stainless steels are widely used in various industrial applications due to their excellence in toughness and corrosion resistance. But their resistance to wear needs to be improved for appropriate use in tribological applications. The Filtered Cathodic Vacuum Arc (FCVA) is a superior technique for forming a high-density film structure of amorphous carbon, especially for a tetrahedral amorphous carbon (ta-C) type, because it can produce a plasma of highly energetic ions that can penetrate into a growing coating, resulting in densification of the film. However, this technique tends to generate high internal stress, due to serious accumulation of energy in the film structure that then leads to film delamination. In general, there are numerous solutions that have been used to reduce the internal stress. DLC with various additive elements such as Ti, Cr or W as strong-carbide-forming (SCF) metals is one of the popular methods to provide attractive combinations of properties of wear resistance and film adhesion as well as reducing the internal stress. The present study was focused on investigation of titanium-doped DLC coating on SS304 steel, mainly for adhesion improvement in optimizing for tribological applications. The synthesized films were formed by the FCVA technique at normal substrate temperature. In the experimental set-up, the films were produced by mixing the titanium and carbon ions generated by dual cathode plasma source operating in synchronous pulsed mode. Their compositions were adjusted by varying the relative duration of the pulse length from each cathode. Titanium doping concentration was varied from pure DLC deposition as the control group to titanium and graphite trigger pulses ratios of 1:16, 1:12, 1:10, 1:8 and 1:4, as the Ti-doped DLC group. The results showed that by increasing titanium trigger pulses ratio from 1:16, 1:12, 1:10 and 1:8, respectively, the film adhesion was increased while the wear rate did not change significantly as measured by scratch test measurement while adjusted more titanium trigger pulses at 1:4 ratio, the wear rate raised rapidly up to be beyond 50%. In summary, the optimized range of Ti doping in DLC structure to maintain both acceptable wear rate and good adhesion properties of FCVA-synthesized Ti-doped DLC was considered to not over 1:8 of titanium and graphite trigger pulses ratio. Mechanism involved in the phenomenon was discussed.

Viral Penetration of High Efficiency Particulate Air (HEPA) Filters (PREPRINT)

DTIC Science & Technology

2009-09-01

US Plastics, Lima , 155 Ohio). Each path runs through a test article and thence through one AGI-30 all-glass 156 impinger (Chemglass, Vineland, N.J...rotameter (Blue–White 400, Huntington Beach , California, or PMR1-159 101346, Cole–Parmer, Vernon Hills, Illinois). At the end of the sampling path...fibrous Filters." J. Air Pollution Control Assoc. 30 [4]: 501 377–381. 502 Leenders, G.J.M, A.C. Bolle, and J. Stadhouders. 1984. “A Study of the

[Evaluation of the viability of BEAS-2B cells exposed to gasoline engine exhaust with different particle sizes by air-liquid interface].

PubMed

Yu, T; Zhang, X Y; Wang, Z X; Li, B; Zheng, Y X; Bin, P

2017-06-20

Objective: To evaluate the viability of gasoline engine exhaust (GEE) with different particle sizes on human lung cell line BEAS-2B in vitro by air-liquid interface (ALI) . Methods: GEE were collected with a Tedlar bag and their particulate matter (PM) number, surface and mass concentration in three kind of GEE (filtered automobile exhaust, non-filtered automobile exhaust and motorcycle exhaust without three-way catalytic converter) were measured by two type of particle size spectrometer including TSI-3321 and SMPS-3938. Five groups were included, which divided into blank control group, clean air group, filtered automobile exhaust group, non-filtered automobile exhaust group and motorcycle exhaust without three-way catalytic converter group. Except the blank control group, BEAS-2B cells, cultured on the surface of Transwells, were treated with clean air or GEE by ALI method at a flow rate of 25 ml/min, 37 ℃ for 60 min in vitro . CCK-8 cytotoxicity test kit was used to determine the cell relative viability of BEAS-2B cells. Results: In the filtered automobile exhaust, non-filtered automobile exhaust and motorcycle exhaust without three-way catalytic converter, high concentrations of fine particles can be detected, but the coarse particles only accounted for a small proportion, and the sequence of PM concentration was motorcycle exhaust without three-way catalytic converter group> non-filtered automobile exhaust group> filtered automobile exhaust group ( P <0.001) . Compared with the clean air group, the cell relative viability in the 3 GEE-exposed groups were significantly lower ( P <0.001) . Among the comparisons of GEE exposure groups with different particle size spectra, the sequence of the cell relative viability was filtered automobile exhaust group >non-filtered automobile exhaust group> motorcycle exhaust without three-way catalytic converter group ( P <0.001) . When took the clean air control group as a reference, the mean of the cell relative viability in the filtered automobile exhaust group, non-filtered automobile exhaust group and motorcycle exhaust without three-way catalytic converter group, was decreased by 26.34%, 36.00% and 49.59%, respectively. Conclusion: GEE with different particle size spectra could induce different levels of toxic effects to the human lung cells BEAS-2B by ALI. After lowering the concentration of particles in the GEE and using the three-way catalytic converter could obviously improve the survival rate of lung cells.

Evaluation of the Long-Term Effect of Air Filtration on the Occurrence of New PRRSV Infections in Large Breeding Herds in Swine-Dense Regions

PubMed Central

Dee, Scott; Cano, Jean Paul; Spronk, Gordon; Reicks, Darwin; Ruen, Paul; Pitkin, Andrea; Polson, Dale

2012-01-01

Airborne transmission of porcine reproductive and respiratory syndrome virus (PRRSV) is a risk factor for the infection of susceptible populations. Therefore, a long‑term sustainability study of air filtration as a means to reduce this risk was conducted. Participating herds (n = 38) were organized into 4 independent cohorts and the effect of air filtration on the occurrence of new PRRSV infections was analyzed at 3 different levels from September 2008 to January 2012 including the likelihood of infection in contemporary filtered and non-filtered herds, the likelihood of infection before and after implementation of filtration and the time to failure in filtered and non-filtered herds. Results indicated that new PRRSV infections in filtered breeding herds were significantly lower than in contemporary non-filtered control herds (P < 0.01), the odds for a new PRRSV infection in breeding herds before filtration was 7.97 times higher than the odds after filtration was initiated (P < 0.01) and the median time to new PRRSV infections in filtered breeding herds of 30 months was significantly longer than the 11 months observed in non-filtered herds (P < 0.01). In conclusion, across all 3 levels of analysis, the long-term effect of air filtration on reducing the occurrence of new PRRSV infections in the study population was demonstrated. PMID:22754642

Filtered sub-grid constitutive models for fluidized gas-particle flows constructed from 3-D simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sarkar, Avik; Milioli, Fernando E.; Ozarkar, Shailesh

2016-10-01

The accuracy of fluidized-bed CFD predictions using the two-fluid model can be improved significantly, even when using coarse grids, by replacing the microscopic kinetic-theory-based closures with coarse-grained constitutive models. These coarse-grained constitutive relationships, called filtered models, account for the unresolved gas-particle structures (clusters and bubbles) via sub-grid corrections. Following the previous 2-D approaches of Igci et al. [AIChE J., 54(6), 1431-1448, 2008] and Milioli et al. [AIChE J., 59(9), 3265-3275, 2013], new filtered models are constructed from highly-resolved 3-D simulations of gas-particle flows. Although qualitatively similar to the older 2-D models, the new 3-D relationships exhibit noticeable quantitative and functionalmore » differences. In particular, the filtered stresses are strongly dependent on the gas-particle slip velocity. Closures for the filtered inter-phase drag, gas- and solids-phase pressures and viscosities are reported. A new model for solids stress anisotropy is also presented. These new filtered 3-D constitutive relationships are better suited to practical coarse-grid 3-D simulations of large, commercial-scale devices.« less

Profile of children and adolescents admitted to a Burn Care Unit in the countryside of the state of São Paulo☆

PubMed Central

Biscegli, Terezinha Soares; Benati, Larissa Delázari; Faria, Rafaela Sperandio; Boeira, Taís Romano; Cid, Felipe Biscegli; Gonsaga, Ricardo Alessandro Teixeira

2014-01-01

Objective: To describe the profile of pediatric burn victims hospitalized at Hospital-Escola Padre Albino (HEPA), in Catanduva, São Paulo, Brazil. Methods: This was a cross-sectional, retrospective study analyzing 446 medical records of patient aged 0-18 years old hospitalized in the Burn Care Unit of HEPA, from 2002 to 2012. The following variables were recorded: demographic data, skin burn causes, lesions characteristics, complications, surgical procedures, length of hospital stay, and outcome. Descriptive statistics were used. Results: 382 patients with full medical records were included in the study. Burns were more frequent in males (64.4%) and in children aged less than 6 years (52.9%). Most accidents occurred at home (67.3%) and hot liquids were responsible for 47.1% of them. Mean burnt body surface was 18% and the most affected body areas were chest and limbs. First- and second-degree burns were observed in 64.4% of the cases. Secondary infection and surgical procedures occurred in 6.5% and 45.0% of the patients, respectively. Mean length of hospital stay was 9.8 days. The mortality rate was 1.6%. Conclusions: Preschool children were the main victims of burns occurring at home, representing the largest contingent of hospitalizations due to this cause in individuals aged < 18 years. It is important to develop strategies to alert parents and general society through educational programs and preventive campaigns. PMID:25479846

Effects of MERV 16 filters and routine work practices on enclosed cabs for reducing respirable dust and DPM exposures in an underground limestone mine

PubMed Central

Noll, J.D.; Cecala, A.B.; J.A.Organiscak; Rider, J.P.

2015-01-01

An effective technique to minimize miners’ respirable dust and diesel exposure on mobile mining equipment is to place mine operators in enclosed cabs with designed filtration and pressurization systems. Many factors affect the performance of these enclosed cab systems, and one of the most significant factors is the effectiveness of the filtration system. High-efficiency particulate air (HEPA)-type filters are typically used because they are highly efficient at capturing all types and sizes of particles, including those in the submicron range such as diesel particulate matter (DPM). However, in laboratory tests, minimum efficiency reporting value (MERV) 16 filters have proven to be highly efficient for capturing DPM and respirable dust. Also, MERV 16 filters can be less restrictive to cab airflow and less expensive than HEPA filters. To verify their effectiveness in the field, MERV 16 filters were used in the enclosed cab filtration system on a face drill and roof bolting mining machine and tested at an underground limestone mine. Test results showed that DPM and respirable dust concentrations were reduced by more than 90% when the cabs were properly sealed. However, when the cab door was opened periodically throughout the shift, the reduction efficiency of the MERV 16 filters was reduced to 80% on average. PMID:26236044

THE ROLE OF CYTOKINES IN UBD PROMOTER REGULATION AND MALLORY-DENK BODY-LIKE AGGRESOMES

PubMed Central

Oliva, Joan; Bardag-Gorce, Fawzia; Lin, Andrew; French, Barbara.A; French, Samuel W.

2010-01-01

Mallory-Denk bodies (MDBs) are found in chronic liver diseases. Previous studies showed that Diethyl-1, 4-dihydro-2,4,6,-trimethyl-3,5-pyridinedicarboxylate (DDC) induced formation of MDBs and the up regulation of UbD expression in mouse liver. UbD is a protein over expressed in hepatocellular carcinomas. It is a potential preneoplastic marker in the mouse. It is hypothesized that inflammatory cytokines play a critical role in UbD up regulation and MDB formation. TNFa and IFNg treatment of HCC cell line Hepa 1–6, induced the expression of UbD and the expression of genes coding for the immunoproteasome (LMP2, LMP7, and MECL-1 subunits). TNFa and IFNg induced the activity of the UbD promoter, using a luciferase assay. The co-treatment with TNFa and IFNg induced the activity of the UbD promoter through an Interferon Sequence Responsive Element (ISRE). In addition, long term treatment with TNFa and IFNg induced the formation of MDB-like aggresomes in Hepa 1–6 cells, which emphasizes the role of inflammation in the formation of MDBs leading to the formation of liver tumors, in the mouse. Identifying the mechanism that regulates gene expression of UbD supports the hypothesis that down regulation of UbD and the proinflammatory gene expression would prevent MDBs and HCC formation. Previous studies indicate that S-adenosylmethionine or betaine prevented IFNg induced UbD and MDB formation. PMID:20433827

49 CFR 572.189 - Instrumentation and test conditions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... mass of the attachments and 1/3 cable mass must not exceed 5 percent of the total pendulum mass. No... lumbar spine pendulum accelerations—Digitally filtered CFC 60; (4) Pelvis, shoulder, thorax without arm...—Digitally filtered at CFC 600; (6) Thorax deflection—Digitally filtered CFC 180. (j)(1) Filter the pendulum...

49 CFR 572.189 - Instrumentation and test conditions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... mass of the attachments and 1/3 cable mass must not exceed 5 percent of the total pendulum mass. No... lumbar spine pendulum accelerations—Digitally filtered CFC 60; (4) Pelvis, shoulder, thorax without arm...—Digitally filtered at CFC 600; (6) Thorax deflection—Digitally filtered CFC 180. (j)(1) Filter the pendulum...

49 CFR 572.189 - Instrumentation and test conditions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... mass of the attachments and 1/3 cable mass must not exceed 5 percent of the total pendulum mass. No... lumbar spine pendulum accelerations—Digitally filtered CFC 60; (4) Pelvis, shoulder, thorax without arm...—Digitally filtered at CFC 600; (6) Thorax deflection—Digitally filtered CFC 180. (j)(1) Filter the pendulum...

Binocular contrast-gain control for natural scenes: Image structure and phase alignment.

PubMed

Huang, Pi-Chun; Dai, Yu-Ming

2018-05-01

In the context of natural scenes, we applied the pattern-masking paradigm to investigate how image structure and phase alignment affect contrast-gain control in binocular vision. We measured the discrimination thresholds of bandpass-filtered natural-scene images (targets) under various types of pedestals. Our first experiment had four pedestal types: bandpass-filtered pedestals, unfiltered pedestals, notch-filtered pedestals (which enabled removal of the spatial frequency), and misaligned pedestals (which involved rotation of unfiltered pedestals). Our second experiment featured six types of pedestals: bandpass-filtered, unfiltered, and notch-filtered pedestals, and the corresponding phase-scrambled pedestals. The thresholds were compared for monocular, binocular, and dichoptic viewing configurations. The bandpass-filtered pedestal and unfiltered pedestals showed classic dipper shapes; the dipper shapes of the notch-filtered, misaligned, and phase-scrambled pedestals were weak. We adopted a two-stage binocular contrast-gain control model to describe our results. We deduced that the phase-alignment information influenced the contrast-gain control mechanism before the binocular summation stage and that the phase-alignment information and structural misalignment information caused relatively strong divisive inhibition in the monocular and interocular suppression stages. When the pedestals were phase-scrambled, the elimination of the interocular suppression processing was the most convincing explanation of the results. Thus, our results indicated that both phase-alignment information and similar image structures cause strong interocular suppression. Copyright © 2018 Elsevier Ltd. All rights reserved.

Development of a pilot system for converting sweet potato starch into glucose syrup

NASA Technical Reports Server (NTRS)

Silayo, Valerian C K.; Lu, John Y.; Aglan, Heshmat A.; Bovell-Benjamin, A. C. (Principal Investigator)

2003-01-01

Sweet potato has been chosen as one of NASA's crops to support human beings in future space missions. One of the possible uses is to make syrup that can be used as a general sweetener. In this work a simple engineering system for converting sweet potato starch into glucose syrup was studied on a laboratory scale. The system comprises the following main units: a blender, continuous stirred tank reactor (CSTR), centrifugal and vacuum filters, deionization column and vacuum evaporator. The system was tested by carrying out conversion processes from fresh sweet potato roots. The roots were pealed, sliced, homogenized, heated and hydrolyzed by diastase of malt and Dextrozyme C (Novo Nordisk BioChem, North America, Inc.) enzymes in the CSTR. After hydrolysis the slurry was filtered, de-ionized and concentrated to get glucose syrup. The performance of the system was evaluated based on the quality of the conversion. The main factor was the level of reducing sugars except for the deionization where ash content and color were the main factors. Through careful control of the system units, good heating performance in the CSTR was obtained and the hydrolysis process attained sufficient conversion. The filtration process that incorporated the centrifuge was faster than when it was by-passed to the vacuum filter but losses in sugars were higher. Deionization removed more than 90% of the ash and reduced pigmentation, with probable insignificant losses in sugars during the deionization process. Recovery levels when the centrifuge was used and when it was by-passed could reach about 65% and 78%, respectively. These correspond to reducing sugar concentration of 259 and 310 mg/ml in 150-ml syrups from 300 g of sweet potatoes in each process. However, from concentration trials, syrups with volumes of 100 and 70 ml with the respective dextrose equivalence of 281 and 213 mg/ml were obtained. The syrups obtained were brownish in color and the process that employed centrifugal filtration gave a product with color that resembled the original color of the sweet potatoes. Further work is required to improve the overall system performance.

Effectiveness of In-Room Air Filtration and Dilution Ventilation for Tuberculosis Infection Control.

PubMed

Miller-Leiden, S; Lohascio, C; Nazaroff, W W; Macher, J M

1996-09-01

Tuberculosis (TB) is a public health problem that may pose substantial risks to health care workers and others. TB infection occurs by inhalation of airborne bacteria emitted by persons with active disease. We experimentally evaluated the effectiveness of in-room air filtration systems, specifically portable air filters (PAFs) and ceiling-mounted air filters (CMAFs), in conjunction with dilution ventilation, for controlling TB exposure in high-risk settings. For each experiment, a test aerosol was continuously generated and released into a full-sized room. With the in-room air filter and room ventilation system operating, time-averaged airborne particle concentrations were measured at several points. The effectiveness of in-room air filtration plus ventilation was determined by comparing particle concentrations with and without device operation. The four PAFs and three CMAFs we evaluated reduced room-average particle concentrations, typically by 30% to 90%, relative to a baseline scenario with two air-changes per hour of ventilation (outside air) only. Increasing the rate of air flow recirculating through the filter and/or air flow from the ventilation did not always increase effectiveness. Concentrations were generally higher near the emission source than elsewhere in the room. Both the air flow configuration of the filter and its placement within the room were important, influencing room air flow patterns and the spatial distribution of concentrations. Air filters containing efficient, but non-high efficiency particulate air (HEPA) filter media were as effective as air filters containing HEPA filter media.

Microplastics, Macroproblems?

NASA Astrophysics Data System (ADS)

Greene, V.; Adams, S.; Adams, A.

2017-12-01

Microplastics and plastics have polluted water all over the world including the Great Lakes. Microplastics can result from plastics that have broken up into smaller pieces or they can be purposely made and used in a variety of products. The size of a microplastic is less than 5 mm in length. These plastics can cause problems because they are non-biodegradable. Animals that have ingested these plastics have had reduced reproductive rates, health problems, and have even died from malnutrition. Our goal is to learn more about this issue. To do this, we will take water samples from different areas along the Gulf of Mexico and inland bays along the Florida coastline and compare the amount of microplastics found in each area. To analyze our samples we will vacuum filter water samples using gridded filter paper. We will then organize these samples by size and color. The control for our experiment will be filtered water. Our hypothesis is that Gulf of Mexico water samples will have more microplastics than the Bay water samples. We want to research this topic because microplastics can harm our ecosystems by affecting the health of marine animals.

A 30 mK, 13.5 T scanning tunneling microscope with two independent tips.

PubMed

Roychowdhury, Anita; Gubrud, M A; Dana, R; Anderson, J R; Lobb, C J; Wellstood, F C; Dreyer, M

2014-04-01

We describe the design, construction, and performance of an ultra-low temperature, high-field scanning tunneling microscope (STM) with two independent tips. The STM is mounted on a dilution refrigerator and operates at a base temperature of 30 mK with magnetic fields of up to 13.5 T. We focus on the design of the two-tip STM head, as well as the sample transfer mechanism, which allows in situ transfer from an ultra high vacuum preparation chamber while the STM is at 1.5 K. Other design details such as the vibration isolation and rf-filtered wiring are also described. Their effectiveness is demonstrated via spectral current noise characteristics and the root mean square roughness of atomic resolution images. The high-field capability is shown by the magnetic field dependence of the superconducting gap of CuxBi2Se3. Finally, we present images and spectroscopy taken with superconducting Nb tips with the refrigerator at 35 mK that indicate that the effective temperature of our tips/sample is approximately 184 mK, corresponding to an energy resolution of 16 μeV.

Monte Carlo derivation of filtered tungsten anode X-ray spectra for dose computation in digital mammography.

PubMed

Paixão, Lucas; Oliveira, Bruno Beraldo; Viloria, Carolina; de Oliveira, Marcio Alves; Teixeira, Maria Helena Araújo; Nogueira, Maria do Socorro

2015-01-01

Derive filtered tungsten X-ray spectra used in digital mammography systems by means of Monte Carlo simulations. Filtered spectra for rhodium filter were obtained for tube potentials between 26 and 32 kV. The half-value layer (HVL) of simulated filtered spectra were compared with those obtained experimentally with a solid state detector Unfors model 8202031-H Xi R/F & MAM Detector Platinum and 8201023-C Xi Base unit Platinum Plus w mAs in a Hologic Selenia Dimensions system using a direct radiography mode. Calculated HVL values showed good agreement as compared with those obtained experimentally. The greatest relative difference between the Monte Carlo calculated HVL values and experimental HVL values was 4%. The results show that the filtered tungsten anode X-ray spectra and the EGSnrc Monte Carlo code can be used for mean glandular dose determination in mammography.

Monte Carlo derivation of filtered tungsten anode X-ray spectra for dose computation in digital mammography*

PubMed Central

Paixão, Lucas; Oliveira, Bruno Beraldo; Viloria, Carolina; de Oliveira, Marcio Alves; Teixeira, Maria Helena Araújo; Nogueira, Maria do Socorro

2015-01-01

Objective Derive filtered tungsten X-ray spectra used in digital mammography systems by means of Monte Carlo simulations. Materials and Methods Filtered spectra for rhodium filter were obtained for tube potentials between 26 and 32 kV. The half-value layer (HVL) of simulated filtered spectra were compared with those obtained experimentally with a solid state detector Unfors model 8202031-H Xi R/F & MAM Detector Platinum and 8201023-C Xi Base unit Platinum Plus w mAs in a Hologic Selenia Dimensions system using a direct radiography mode. Results Calculated HVL values showed good agreement as compared with those obtained experimentally. The greatest relative difference between the Monte Carlo calculated HVL values and experimental HVL values was 4%. Conclusion The results show that the filtered tungsten anode X-ray spectra and the EGSnrc Monte Carlo code can be used for mean glandular dose determination in mammography. PMID:26811553

Limited retention of micro-organisms using commercialized needle filters.

PubMed

Elbaz, W; McCarthy, G; Mawhinney, T; Goldsmith, C E; Moore, J E

2015-03-01

A study was undertaken to compare a commercialized needle filter with a 0.2-μm filtered epidural set and a non-filtered standard needle. No culturable bacteria were detected following filtration through the 0.2-μm filter. Bacterial breakthrough was observed with the filtered needle (pore size 5 μm) and the non-filtered needle. Filtered systems (0.2 μm) should be employed to achieve total bacterial retention. This highlights that filtration systems with different pore sizes will have varying ability to retain bacteria. Healthcare professionals need to know what type/capability of filter is implied on labels used by manufacturers, and to assess whether the specification has the desired functionality to prevent bacterial translocation through needles. Copyright © 2015 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

Development of Test Protocols for International Space Station Particulate Filters

NASA Technical Reports Server (NTRS)

Green, Robert D.; Vijayakumar, R.; Agui, Juan H.

2014-01-01

Air quality control on the International Space Station (ISS) is a vital requirement for maintaining a clean environment for the crew and the hardware. This becomes a serious challenge in pressurized space compartments since no outside air ventilation is possible, and a larger particulate load is imposed on the filtration system due to lack of gravitational settling. The ISS Environmental Control and Life Support System (ECLSS) uses a filtration system that has been in use for over 14 years and has proven to meet this challenge. The heart of this system is a traditional High- Efficiency Particulate Air (HEPA) filter configured to interface with the rest of the life support elements and provide effective cabin filtration. Over the years, the service life of these filters has been re-evaluated based on limited post-flight tests of returned filters and risk factors. On earth, a well designed and installed HEPA filter will last for several years, e.g. in industrial and research clean room applications. Test methods for evaluating these filters are being developed on the basis of established test protocols used by the industry and the military. This paper will discuss the test methods adopted and test results on prototypes of the ISS filters. The results will assist in establishing whether the service life can be extended for these filters. Results from unused filters that have been in storage will also be presented to ascertain the shelf life and performance deterioration, if any and determine if the shelf life may be extended.

Integrated Stirling Convertor and Hall Thruster Test Conducted

NASA Technical Reports Server (NTRS)

Mason, Lee S.

2002-01-01

An important aspect of implementing Stirling Radioisotope Generators on future NASA missions is the integration of the generator and controller with potential spacecraft loads. Some recent studies have indicated that the combination of Stirling Radioisotope Generators and electric propulsion devices offer significant trip time and payload fraction benefits for deep space missions. A test was devised to begin to understand the interactions between Stirling generators and electric thrusters. An electrically heated RG- 350 (350-W output) Stirling convertor, designed and built by Stirling Technology Company of Kennewick, Washington, under a NASA Small Business Innovation Research agreement, was coupled to a 300-W SPT-50 Hall-effect thruster built for NASA by the Moscow Aviation Institute (RIAME). The RG-350 and the SPT-50 shown, were installed in adjacent vacuum chamber ports at NASA Glenn Research Center's Electric Propulsion Laboratory, Vacuum Facility 8. The Stirling electrical controller interfaced directly with the Hall thruster power-processing unit, both of which were located outside of the vacuum chamber. The power-processing unit accepted the 48 Vdc output from the Stirling controller and distributed the power to all the loads of the SPT-50, including the magnets, keeper, heater, and discharge. On February 28, 2001, the Glenn test team successfully operated the Hall-effect thruster with the Stirling convertor. This is the world's first known test of a dynamic power source with electric propulsion. The RG-350 successfully managed the transition from the purely resistive load bank within the Stirling controller to the highly capacitive power-processing unit load. At the time of the demonstration, the Stirling convertor was operating at a hot temperature of 530 C and a cold temperature of -6 C. The linear alternator was producing approximately 250 W at 109 Vac, while the power-processing unit was drawing 175 W at 48 Vdc. The majority of power was delivered to the Hall thruster discharge circuit operating at 115 Vdc and 0.9 A. Testing planned for late 2001 will examine the possibility of directly driving the Hall thruster discharge circuit using rectified and filtered output from the Stirling alternator.

Sound source localization identification accuracy: Envelope dependencies.

PubMed

Yost, William A

2017-07-01

Sound source localization accuracy as measured in an identification procedure in a front azimuth sound field was studied for click trains, modulated noises, and a modulated tonal carrier. Sound source localization accuracy was determined as a function of the number of clicks in a 64 Hz click train and click rate for a 500 ms duration click train. The clicks were either broadband or high-pass filtered. Sound source localization accuracy was also measured for a single broadband filtered click and compared to a similar broadband filtered, short-duration noise. Sound source localization accuracy was determined as a function of sinusoidal amplitude modulation and the "transposed" process of modulation of filtered noises and a 4 kHz tone. Different rates (16 to 512 Hz) of modulation (including unmodulated conditions) were used. Providing modulation for filtered click stimuli, filtered noises, and the 4 kHz tone had, at most, a very small effect on sound source localization accuracy. These data suggest that amplitude modulation, while providing information about interaural time differences in headphone studies, does not have much influence on sound source localization accuracy in a sound field.

A priori and a posteriori investigations for developing large eddy simulations of multi-species turbulent mixing under high-pressure conditions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Borghesi, Giulio; Bellan, Josette, E-mail: josette.bellan@jpl.nasa.gov; Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California 91109-8099

2015-03-15

A Direct Numerical Simulation (DNS) database was created representing mixing of species under high-pressure conditions. The configuration considered is that of a temporally evolving mixing layer. The database was examined and analyzed for the purpose of modeling some of the unclosed terms that appear in the Large Eddy Simulation (LES) equations. Several metrics are used to understand the LES modeling requirements. First, a statistical analysis of the DNS-database large-scale flow structures was performed to provide a metric for probing the accuracy of the proposed LES models as the flow fields obtained from accurate LESs should contain structures of morphology statisticallymore » similar to those observed in the filtered-and-coarsened DNS (FC-DNS) fields. To characterize the morphology of the large-scales structures, the Minkowski functionals of the iso-surfaces were evaluated for two different fields: the second-invariant of the rate of deformation tensor and the irreversible entropy production rate. To remove the presence of the small flow scales, both of these fields were computed using the FC-DNS solutions. It was found that the large-scale structures of the irreversible entropy production rate exhibit higher morphological complexity than those of the second invariant of the rate of deformation tensor, indicating that the burden of modeling will be on recovering the thermodynamic fields. Second, to evaluate the physical effects which must be modeled at the subfilter scale, an a priori analysis was conducted. This a priori analysis, conducted in the coarse-grid LES regime, revealed that standard closures for the filtered pressure, the filtered heat flux, and the filtered species mass fluxes, in which a filtered function of a variable is equal to the function of the filtered variable, may no longer be valid for the high-pressure flows considered in this study. The terms requiring modeling are the filtered pressure, the filtered heat flux, the filtered pressure work, and the filtered species mass fluxes. Improved models were developed based on a scale-similarity approach and were found to perform considerably better than the classical ones. These improved models were also assessed in an a posteriori study. Different combinations of the standard models and the improved ones were tested. At the relatively small Reynolds numbers achievable in DNS and at the relatively small filter widths used here, the standard models for the filtered pressure, the filtered heat flux, and the filtered species fluxes were found to yield accurate results for the morphology of the large-scale structures present in the flow. Analysis of the temporal evolution of several volume-averaged quantities representative of the mixing layer growth, and of the cross-stream variation of homogeneous-plane averages and second-order correlations, as well as of visualizations, indicated that the models performed equivalently for the conditions of the simulations. The expectation is that at the much larger Reynolds numbers and much larger filter widths used in practical applications, the improved models will have much more accurate performance than the standard one.« less

Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus

PubMed Central

Shiwani, Supriya; Singh, Naresh Kumar

2012-01-01

The study elucidated carbohydrase inhibition, anti-cancerous, free radical scavenging properties and also investigated the DNA and protein protection abilities of methanolic root extract of Rumex crispus (RERC). For this purpose, pulverized roots of Rumex crispus was extracted in methanol (80% and absolute conc.) for 3 hrs for 60℃ and filtered and evaporated with vacuum rotary evaporator. RERC showed high phenolic content (211 µg/GAE equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC50 = 42.86 (absolute methanol) and 36.91 µg/mL (80% methanolic extract)) and reduced power ability. Furthermore, RERC exhibited significant protective ability in H2O2/Fe3+/ascorbic acid-induced protein or DNA damage and percentage inhibition of the HT-29 cell growth rate following 80% methanolic RERC exposure at 400 µg/mL was observed to be highest (10.2% ± 1.03). Moreover, methanolic RERC inhibited α-glucosidase and amylase effectively and significantly (P < 0.05). Conclusively, RERC could be considered as potent carbohydrase inhibitor, anti-cancerous and anti-oxidant. PMID:23198017

Fast imaging measurements and modeling of neutral and impurity density on C-2U

NASA Astrophysics Data System (ADS)

Granstedt, Erik; Deng, B.; Dettrick, S.; Gupta, D. K.; Osin, D.; Roche, T.; Zhai, K.; TAE Team

2016-10-01

The C-2U device employed neutral beam injection and end-biasing to sustain an advanced beam-driven Field-Reversed Configuration plasma for 5+ ms, beyond characteristic transport time-scales. Three high-speed, filtered cameras observed visible light emission from neutral hydrogen and impurities, as well as deuterium pellet ablation and compact-toroid injection which were used for auxiliary particle fueling. Careful vacuum practices and titanium gettering successfully reduced neutral recycling from the confinement vessel wall. As a result, a large fraction of the remaining neutrals originate from charge-exchange between the neutral beams and plasma ions. Measured H/D- α emission is used with DEGAS2 neutral particle modeling to reconstruct the strongly non-axissymmetric neutral distribution. This is then used in fast-ion modeling to more accurately estimate their charge-exchange loss rate. Oxygen emission due to electron-impact excitation and charge-exchange recombination has also been measured using fast imaging. Reconstructed emissivity of O4+ is localized on the outboard side of the core plasma near the estimated location of the separatrix inferred by external magnetic measurements. Tri Alpha Energy.

Glut-1 as a therapeutic target: increased chemoresistance and HIF-1-independent link with cell turnover is revealed through COMPARE analysis and metabolomic studies.

PubMed

Evans, Andrew; Bates, Victoria; Troy, Helen; Hewitt, Stephen; Holbeck, Susan; Chung, Yuen-Li; Phillips, Roger; Stubbs, Marion; Griffiths, John; Airley, Rachel

2008-03-01

The facilitative glucose transporter Glut-1 is overexpressed and confers poor prognosis in a wide range of solid tumours. The peri-necrotic pattern of expression often seen in human tumour samples is linked with its transcriptional control in hypoxic conditions by hypoxia-inducible factor HIF-1 or through a reduced rate of oxidative phosphorylation. Hypoxia-regulated genes offer promise as novel therapeutic targets as a means of preventing the proliferation and eventual metastatic spread of tissue originating from residual chemically and radio resistant hypoxic cells that have survived treatment. Inhibiting the expression or functionality of Glut-1 may be a way of specifically targeting hypoxic cells within the tumour that depend upon a high rate of glucose uptake for anaerobic glycolysis. We used an array of formalin-fixed, paraffin-embedded samples of the NCI-60 panel of cell lines to carry out immunohistochemical detection of Glut-1 and to select possible candidate lead compounds by COMPARE analysis with agents from the NCI diversity screen, which may work via inhibition of Glut-1 or Glut-1-dependent processes. "Positive" COMPARE hits were mostly conjugated Pseudomonas toxins binding the epidermal growth factor receptor (EGFR). However, correlations with standard anticancer agents were virtually all negative, indicating a link between Glut-1 and chemoresistance. MTT proliferation assays carried out using stable, Glut-1 overexpressing cell lines generated from the bladder EJ138, human fibrosarcoma HT 1080 and the hepatoma wild type Hepa and HIF-1B-deficient c4 tumour cell lines revealed a cell line-dependent increase in chemoresistance to dacarbazine, vincristine and the bioreductive agent EO9 in Glut-1 overexpressing EJ138 relative to WT and empty vector controls. Metabolomic analysis ((31)P-MRS and (1)H MRS) carried out using cell lysates and xenografts generated from Glut-1 overexpressing Hepa and c4 cell lines showed higher glucose levels in Glut-1 overxpressing c4 relative to parental tumour extracts occurred in the absence of an increase in lactate levels, which were in turn significantly higher in the Glut-1 overexpressing Hepa xenografts. This implies that Glut-1 over-expression without a co-ordinate increase in HIF-1-regulated glycolytic enzymes increases glucose uptake but not the rate of glycolysis. Glut-1 overexpressing xenografts also showed higher levels of phosphodiester (PDE), which relates to the metabolite turnover of phospholipids and is involved in membrane lipid degradation, indicating a mechanism by which Glut-1 may increase cell turnover.

Ultrahigh performance heat spreader based on gas-liquid exfoliation boron nitride nanosheets.

PubMed

Ding, Jiheng; Zhao, Hongran; Wang, Qiaolei; Peng, Wanjun; Yu, Haibin

2017-11-24

Due to their high thermal conductivity and insulation performance, boron nitride nanosheets (BNNS) have great promise to fabricate thermal management equipment for application in power electronics. The liquid-phase exfoliation route has been regarded as the most commonly used approach to produce single and few-layered BNNS for many research fields. However, this process takes a long time, and the production yield is extremely low. In this work, an efficient technique to obtain few-layered (mostly < 5 layers), high-yield (∼33%), and plane-defect-free BNNS by the combination of liquid N 2 (L-N 2 ) gasification and liquid exfoliation was developed. The as-obtained BNNS suspensions could be vacuum filtered to make a thermal conductive film named a BNNS heat spreader which possessed a superior thermal conductivity of 61.2 W m -1 K -1 at room temperature. In addition, we also proved that the thermal conductivity of the BNNS heat spreader increased with the increase of density, creating an approach for fine tuning the thermal property of this heat spreader.

A 30 mK, 13.5 T scanning tunneling microscope with two independent tips

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roychowdhury, Anita; Center for Nanophysics and Advanced Materials, Department of Physics, University of Maryland, College Park, Maryland 20740; Gubrud, M. A.

We describe the design, construction, and performance of an ultra-low temperature, high-field scanning tunneling microscope (STM) with two independent tips. The STM is mounted on a dilution refrigerator and operates at a base temperature of 30 mK with magnetic fields of up to 13.5 T. We focus on the design of the two-tip STM head, as well as the sample transfer mechanism, which allows in situ transfer from an ultra high vacuum preparation chamber while the STM is at 1.5 K. Other design details such as the vibration isolation and rf-filtered wiring are also described. Their effectiveness is demonstrated viamore » spectral current noise characteristics and the root mean square roughness of atomic resolution images. The high-field capability is shown by the magnetic field dependence of the superconducting gap of Cu{sub x}Bi{sub 2}Se{sub 3}. Finally, we present images and spectroscopy taken with superconducting Nb tips with the refrigerator at 35 mK that indicate that the effective temperature of our tips/sample is approximately 184 mK, corresponding to an energy resolution of 16 μeV.« less

Comprehensive isolation of natural organic matter from water for spectral characterizations and reactivity testing

USGS Publications Warehouse

Leenheer, J.A.; Croue, J.-P.; Benjamin, M.; Korshin, G.V.; Hwang, C.J.; Bruchet, A.; Aiken, G.R.

2000-01-01

A variety of approaches were tested to comprehensively isolate natural organic matter (NOM) from water. For waters with high NOM concentrations such as the Suwannee River, Georgia, approaches that used combinations of membrane concentrations, evaporative concentrations, and adsorption on nonionic XAD resins, ion exchange resins and iron oxide coated sand isolated over 90% of the NOM. However, for waters with low NOM concentrations, losses of half of the NOM were common and desalting of NOM isolates was a problem. A new comprehensive approach was devised and tested on the Seine River, France in which 100 L of filtered water was sodium softened by ion exchange and vacuum evaporated to 100 mL. Colloids (32% of the NOM) were isolated using a 3,500 Dalton membrane by dialysis against 0.1 M HCl and 0.2 M HF to remove salts and silica. On the membrane permeate, hydrophobic NOM (42%) was isolated using XAD-8 resin and hydrophilic NOM (26%) was isolated using a variety of selective desalting precipitations. The colloid fraction was characterized by IR and NMR spectroscopy as N-acetylamino sugars. ?? 2000 American Chemical Society.

Filtered epithermal quasi-monoenergetic neutron beams at research reactor facilities.

PubMed

Mansy, M S; Bashter, I I; El-Mesiry, M S; Habib, N; Adib, M

2015-03-01

Filtered neutron techniques were applied to produce quasi-monoenergetic neutron beams in the energy range of 1.5-133keV at research reactors. A simulation study was performed to characterize the filter components and transmitted beam lines. The filtered beams were characterized in terms of the optimal thickness of the main and additive components. The filtered neutron beams had high purity and intensity, with low contamination from the accompanying thermal emission, fast neutrons and γ-rays. A computer code named "QMNB" was developed in the "MATLAB" programming language to perform the required calculations. Copyright © 2014 Elsevier Ltd. All rights reserved.

Diel mercury-concentration variations in streams affected by mining and geothermal discharge

USGS Publications Warehouse

Nimick, D.A.; McCleskey, R. Blaine; Gammons, C.H.; Cleasby, T.E.; Parker, S.R.

2007-01-01

Diel variations of concentrations of unfiltered and filtered total Hg and filtered methyl Hg were documented during 24-h sampling episodes in water from Silver Creek, which drains a historical gold-mining district near Helena, Montana, and the Madison River, which drains the geothermal system of Yellowstone National Park. The concentrations of filtered methyl Hg had relatively large diel variations (increases of 68 and 93% from morning minima) in both streams. Unfiltered and filtered (0.1-??m filtration) total Hg in Silver Creek had diel concentration increases of 24% and 7%, respectively. In the Madison River, concentrations of unfiltered and filtered total Hg did not change during the sampling period. The concentration variation of unfiltered total Hg in Silver Creek followed the diel variation in suspended-particle concentration. The concentration variation of filtered total and methyl Hg followed the solar photocycle, with highest concentrations during the early afternoon and evening and lowest concentrations during the morning. None of the diel Hg variations correlated with diel variation in streamflow or major ion concentrations. The diel variation in filtered total Hg could have been produced by adsorption-desorption of Hg2+ or by reduction of Hg(II) to Hg0 and subsequent evasion of Hg0. The diel variation in filtered methyl Hg could have been produced by sunlight- and temperature-dependent methylation. This study is the first to examine diel Hg cycling in streams, and its results reinforce previous conclusions that diel trace-element cycling in streams is widespread but often not recognized and that parts of the biogeochemical Hg cycle respond quickly to the daily photocycle. ?? 2006 Elsevier B.V. All rights reserved.

Effectiveness of in-room air filtration and dilution ventilation for tuberculosis infection control.

PubMed

Miller-Leiden, S; Lobascio, C; Nazaroff, W W; Macher, J M

1996-09-01

Tuberculosis (TB) is a public health problem that may pose substantial risks to health care workers and others. TB infection occurs by inhalation of airborne bacteria emitted by persons with active disease. We experimentally evaluated the effectiveness of in-room air filtration systems, specifically portable air filters (PAFs) and ceiling-mounted air filters (CMAFs), in conjunction with dilution ventilation, for controlling TB exposure in high-risk settings. For each experiment, a test aerosol was continuously generated and released into a full-sized room. With the in-room air filter and room ventilation system operating, time-averaged airborne particle concentrations were measured at several points. The effectiveness of in-room air filtration plus ventilation was determined by comparing particle concentrations with and without device operation. The four PAFs and three CMAFs we evaluated reduced room-average particle concentrations, typically by 30% to 90%, relative to a baseline scenario with two air-changes per hour of ventilation (outside air) only. Increasing the rate of air flow recirculating through the filter and/or air flow from the ventilation did not always increase effectiveness. Concentrations were generally higher near the emission source than elsewhere in the room. Both the air flow configuration of the filter and its placement within the room were important, influencing room air flow patterns and the spatial distribution of concentrations. Air filters containing efficient, but non-high efficiency particulate air (HEPA) filter media were as effective as air filters containing HEPA filter media.

Results from Evaluation of Proposed ASME AG-1 Section FI Metal Media Filters - 13063

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, John A.; Giffin, Paxton K.; Parsons, Michael S.

High efficiency particulate air (HEPA) filtration technology is commonly used in Department of Energy (DOE) facilities that require control of radioactive particulate matter (PM) emissions due to treatment or management of radioactive materials. Although HEPA technology typically makes use of glass fiber media, metal and ceramic media filters are also capable of filtering efficiencies beyond the required 99.97%. Sintered metal fiber filters are good candidates for use in DOE facilities due to their resistance to corrosive environments and resilience at high temperature and elevated levels of relative humidity. Their strength can protect them from high differential pressure or pressure spikesmore » and allow for back pulse cleaning, extending filter lifetime. Use of these filters has the potential to reduce the cost of filtration in DOE facilities due to life cycle cost savings. ASME AG-1 section FI has not been approved due to a lack of protocols and performance criteria for qualifying section FI filters. The Institute for Clean Energy Technology (ICET) with the aid of the FI project team has developed a Section FI test stand and test plan capable of assisting in the qualification ASME AG-1 section FI filters. Testing done at ICET using the FI test stand evaluates resistance to rated air flow, test aerosol penetration and resistance to heated air of the section FI filters. Data collected during this testing consists of temperature, relative humidity, differential pressure, flow rate, upstream particle concentration, and downstream particle concentration. (authors)« less

On low-frequency errors of uniformly modulated filtered white-noise models for ground motions

USGS Publications Warehouse

Safak, Erdal; Boore, David M.

1988-01-01

Low-frequency errors of a commonly used non-stationary stochastic model (uniformly modulated filtered white-noise model) for earthquake ground motions are investigated. It is shown both analytically and by numerical simulation that uniformly modulated filter white-noise-type models systematically overestimate the spectral response for periods longer than the effective duration of the earthquake, because of the built-in low-frequency errors in the model. The errors, which are significant for low-magnitude short-duration earthquakes, can be eliminated by using the filtered shot-noise-type models (i. e. white noise, modulated by the envelope first, and then filtered).

On pads and filters: Processing strong-motion data

USGS Publications Warehouse

Boore, D.M.

2005-01-01

Processing of strong-motion data in many cases can be as straightforward as filtering the acceleration time series and integrating to obtain velocity and displacement. To avoid the introduction of spurious low-frequency noise in quantities derived from the filtered accelerations, however, care must be taken to append zero pads of adequate length to the beginning and end of the segment of recorded data. These padded sections of the filtered acceleration need to be retained when deriving velocities, displacements, Fourier spectra, and response spectra. In addition, these padded and filtered sections should also be included in the time series used in the dynamic analysis of structures and soils to ensure compatibility with the filtered accelerations.

Organophosphates in aircraft cabin and cockpit air--method development and measurements of contaminants.

PubMed

Solbu, Kasper; Daae, Hanne Line; Olsen, Raymond; Thorud, Syvert; Ellingsen, Dag Gunnar; Lindgren, Torsten; Bakke, Berit; Lundanes, Elsa; Molander, Paal

2011-05-01

Methods for measurements and the potential for occupational exposure to organophosphates (OPs) originating from turbine and hydraulic oils among flying personnel in the aviation industry are described. Different sampling methods were applied, including active within-day methods for OPs and VOCs, newly developed passive long-term sample methods (deposition of OPs to wipe surface areas and to activated charcoal cloths), and measurements of OPs in high-efficiency particulate air (HEPA) recirculation filters (n = 6). In total, 95 and 72 within-day OP and VOC samples, respectively, have been collected during 47 flights in six different models of turbine jet engine, propeller and helicopter aircrafts (n = 40). In general, the OP air levels from the within-day samples were low. The most relevant OP in this regard originating from turbine and engine oils, tricresyl phosphate (TCP), was detected in only 4% of the samples (min-max

An observational study of spectators’ step counts and reasons for attending a professional golf tournament in Scotland

PubMed Central

Murray, Andrew D; Turner, Kieran; Archibald, Daryll; Schiphorst, Chloe; Griffin, Steffan Arthur; Scott, Hilary; Hawkes, Roger; Kelly, Paul; Grant, Liz; Mutrie, Nanette

2017-01-01

Background Spectators at several hundred golf tournaments on six continents worldwide may gain health-enhancing physical activity (HEPA) during their time at the event. This study aims to investigate spectators’ reasons for attending and assess spectator physical activity (PA) (measured by step count). Methods Spectators at the Paul Lawrie Matchplay event in Scotland (August 2016) were invited to take part in this study. They were asked to complete a brief questionnaire with items to assess (1) demographics, (2) reasons for attendance and (3) baseline PA. In addition, participants were requested to wear a pedometer from time of entry to the venue until exit. Results A total of 339 spectators were recruited to the study and out of which 329 (97.2%) returned step-count data. Spectators took a mean of 11 589 steps (SD 4531). ‘Fresh air’ (rated median 9 out of 10) then ‘watching star players’, ‘exercise/physical activity’, ‘time with friends and family’ and ‘atmosphere’ (all median 8 out of 10) were rated the most important reasons for attending. Conclusion This study is the first to assess spectator physical activity while watching golf (measured by step count). Obtaining exercise/PA is rated as an important reason for attending a tournament by many golf spectators. Spectating at a golf tournament can provide HEPA. 82.9% of spectators achieved the recommended daily step count while spectating. Further research directly assessing whether spectating may constitute a ‘teachable moment’, for increasing physical activity beyond the tournament itself, is merited. PMID:28761718

On the concept of a filtered bundle

NASA Astrophysics Data System (ADS)

Bruce, Andrew James; Grabowska, Katarzyna; Grabowski, Janusz

We present the notion of a filtered bundle as a generalization of a graded bundle. In particular, we weaken the necessity of the transformation laws for local coordinates to exactly respect the weight of the coordinates by allowing more general polynomial transformation laws. The key examples of such bundles include affine bundles and various jet bundles, both of which play fundamental roles in geometric mechanics and classical field theory. We also present the notion of double filtered bundles which provide natural generalizations of double vector bundles and double affine bundles. Furthermore, we show that the linearization of a filtered bundle — which can be seen as a partial polarization of the admissible changes of local coordinates — is well defined.

The role of low-spatial frequencies in lexical decision and masked priming.

PubMed

Boden, C; Giaschi, D

2009-04-01

Spatial frequency filtering was used to test the hypotheses that low-spatial frequency information in printed text can: (1) lead to a rapid lexical decision or (2) facilitate word recognition. Adult proficient readers made lexical decisions in unprimed and masked repetition priming experiments with unfiltered, low-pass, high-pass and notch filtered letter strings. In the unprimed experiments, a filtered target was presented for 105 or 400 ms followed by a pattern mask. Sensitivity (d') was lowest for the low-pass filtered targets at both durations with a bias towards a 'non-word' response. Sensitivity was higher in the high-pass and notch filter conditions. In the priming experiments, a forward mask was followed by a filtered prime then an unfiltered target. Primed words, but not non-words, were identified faster than unprimed words in both the low-pass and high-pass filtered conditions. These results do not support a unique role for low-spatial frequency information in either facilitating or making rapid lexical decisions.

Microbial Anomalies Encountered on the International Space Station

NASA Technical Reports Server (NTRS)

Bruce, Rebekah J.; Wong, Wing; Pierson, Duane; Castro, Victoria

2010-01-01

Microorganisms in our living environments are unavoidable. A community of microbes arrived in space with the delivery of the first element of the International Space Station (ISS), attached to hardware and on the bodies of the humans tasked with the initial assembly missions. The risk that microorganisms could cause adverse effects in the health of both the human occupants of the ISS as well as the physical integrity of the station environment and life support systems has been both a driver and a function of engineering and operational controls. Scientists and engineers at NASA have gone to extensive measures to control microbial growth at levels safe for the crewmembers and the spacecraft environment. Many of these measures were initiated with the design of the spacecraft and its systems. Materials used in the ISS were tested for resistance to fungi, such as mold and a paint with a fungus-killing chemical was also used. Controlling the humidity of the air in the Station is also an effective way of discouraging microbe growth. The breathing air is reconditioned by the Environmental Control Life Support System (ECLSS) prior to distribution, utilizing High Efficiency Particulate Air (HEPA) filtration. Requirements restricting the accumulation of water condensate in the air handlers and habitable volume of the ISS were other safeguards added. Water for drinking and food rehydration is disinfected or filtered. A robust in-flight housekeeping regimen for the ISS significantly reduces inappropriate growth of microorganisms and includes a regular cleaning of accessible surfaces with disinfectant wipes. Most of these requirements were suggested by microbiologists to mitigate and possibly prevent many microbiological risks. In addition to these controls, before flight monitoring and analyses of the cabin air, exposed surfaces, water and food, consumables, and crew members are conducted to mitigate microbial risk to the crew and spacecraft. Many microbial risks are much easier to identify and resolve before launch than during space flight. Although the focus has been on prevention of microbiologically related, not all problems can be anticipated. A number of microbial anomalies have occurred on ISS. This paper will discuss the occurrences, root-cause investigations, and mitigation steps taken to remediate the contamination.

Control of experimental uncertainties in filtered Rayleigh scattering measurements

NASA Technical Reports Server (NTRS)

Forkey, Joseph N.; Finkelstein, N. D.; Lempert, Walter R.; Miles, Richard B.

1995-01-01

Filtered Rayleigh Scattering is a technique which allows for measurement of velocity, temperature, and pressure in unseeded flows, spatially resolved in 2-dimensions. We present an overview of the major components of a Filtered Rayleigh Scattering system. In particular, we develop and discuss a detailed theoretical model along with associated model parameters and related uncertainties. Based on this model, we then present experimental results for ambient room air and for a Mach 2 free jet, including spatially resolved measurements of velocity, temperature, and pressure.

Sub-basin-scale sea level budgets from satellite altimetry, Argo floats and satellite gravimetry: a case study in the North Atlantic Ocean

NASA Astrophysics Data System (ADS)

Kleinherenbrink, Marcel; Riva, Riccardo; Sun, Yu

2016-11-01

In this study, for the first time, an attempt is made to close the sea level budget on a sub-basin scale in terms of trend and amplitude of the annual cycle. We also compare the residual time series after removing the trend, the semiannual and the annual signals. To obtain errors for altimetry and Argo, full variance-covariance matrices are computed using correlation functions and their errors are fully propagated. For altimetry, we apply a geographically dependent intermission bias [Ablain et al.(2015)], which leads to differences in trends up to 0.8 mm yr-1. Since Argo float measurements are non-homogeneously spaced, steric sea levels are first objectively interpolated onto a grid before averaging. For the Gravity Recovery And Climate Experiment (GRACE), gravity fields full variance-covariance matrices are used to propagate errors and statistically filter the gravity fields. We use four different filtered gravity field solutions and determine which post-processing strategy is best for budget closure. As a reference, the standard 96 degree Dense Decorrelation Kernel-5 (DDK5)-filtered Center for Space Research (CSR) solution is used to compute the mass component (MC). A comparison is made with two anisotropic Wiener-filtered CSR solutions up to degree and order 60 and 96 and a Wiener-filtered 90 degree ITSG solution. Budgets are computed for 10 polygons in the North Atlantic Ocean, defined in a way that the error on the trend of the MC plus steric sea level remains within 1 mm yr-1. Using the anisotropic Wiener filter on CSR gravity fields expanded up to spherical harmonic degree 96, it is possible to close the sea level budget in 9 of 10 sub-basins in terms of trend. Wiener-filtered Institute of Theoretical geodesy and Satellite Geodesy (ITSG) and the standard DDK5-filtered CSR solutions also close the trend budget if a glacial isostatic adjustment (GIA) correction error of 10-20 % is applied; however, the performance of the DDK5-filtered solution strongly depends on the orientation of the polygon due to residual striping. In 7 of 10 sub-basins, the budget of the annual cycle is closed, using the DDK5-filtered CSR or the Wiener-filtered ITSG solutions. The Wiener-filtered 60 and 96 degree CSR solutions, in combination with Argo, lack amplitude and suffer from what appears to be hydrological leakage in the Amazon and Sahel regions. After reducing the trend, the semiannual and the annual signals, 24-53 % of the residual variance in altimetry-derived sea level time series is explained by the combination of Argo steric sea levels and the Wiener-filtered ITSG MC. Based on this, we believe that the best overall solution for the MC of the sub-basin-scale budgets is the Wiener-filtered ITSG gravity fields. The interannual variability is primarily a steric signal in the North Atlantic Ocean, so for this the choice of filter and gravity field solution is not really significant.

Two-wavelength spatial-heterodyne holography

DOEpatents

Hanson, Gregory R.; Bingham, Philip R.; Simpson, John T.; Karnowski, Thomas P.; Voelkl, Edgar

2007-12-25

Systems and methods are described for obtaining two-wavelength differential-phase holograms. A method includes determining a difference between a filtered analyzed recorded first spatially heterodyne hologram phase and a filtered analyzed recorded second spatially-heterodyned hologram phase.

Next generation 1536-well oligonucleotide synthesizer with on-the-fly dispense.

PubMed

Jensen, Michael; Roberts, Lester; Johnson, Andrew; Fukushima, Marilyn; Davis, Ronald

2014-02-10

Here we report the development of our Next Generation Automated Multiplexed Oligonucleotide Synthesizer (NG-1536-AMOS), capable of producing 1536 samples in a single run using a multi-well filtered titer plate. With the potential to synthesize up to 3456 samples per plate, we converted the BioRAPTR Flying Reagent Dispenser into an open-well system where spent reagents are drained to waste under vacuum. During synthesis, reagents are delivered on-the-fly to each micro-titer well at volumes ≤5 μl with plate speeds up to 150 mm/s. Using gas-phase cleavage and deprotection, a full plate of 1536 60 mers may be processed with same-day turnaround with an average yield per well at 3.5 nmol. Final product at only $0.00277/base is eluted into a low-volume collection plate for immediate use in downstream application (e.g. Biomek FX for versatile sample handling). Also, crude oligonucleotide quality is comparable to that of commercial synthesis instrumentation, with an error rate on the NG-1536-AMOS platform of 1.53/717 bases. Furthermore, mass spectral analysis on strands synthesized up to 80 bases showed high purity with an average coupling efficiency of 99.5%. Copyright © 2013 Elsevier B.V. All rights reserved.

Mesh-Sequenced Realizations for Evaluation of Subgrid-Scale Models for Turbulent Combustion (Short Term Innovative Research Program)

DTIC Science & Technology

2018-02-15

conservation equations. The closure problem hinges on the evaluation of the filtered chemical production rates. In MRA/MSR, simultaneous large-eddy...simulations of a reactive flow are performed at different mesh resolution levels. The solutions at each coarser mesh level are constrained by the filtered ...include the replacement of chemical production rates with those filtered from the underlying fine mesh and the construction of ‘exact’ forms for

Identification of a Class of Filtered Poisson Processes.

DTIC Science & Technology

1981-01-01

LD-A135 371 IDENTIFICATION OF A CLASS OF FILERED POISSON PROCESSES I AU) NORTH CAROLINA UNIV AT CHAPEL HIL DEPT 0F STATISTICS D DE RRUC ET AL 1981...STNO&IO$ !tt ~ 4.s " . , ".7" -L N ~ TITLE :IDENTIFICATION OF A CLASS OF FILTERED POISSON PROCESSES Authors : DE BRUCQ Denis - GUALTIEROTTI Antonio...filtered Poisson processes is intro- duced : the amplitude has a law which is spherically invariant and the filter is real, linear and causal. It is shown

Metabotropic glutamate receptor 5 mediates the suppressive effect of 6-OHDA-induced model of Parkinson's disease on liver cancer.

PubMed

Xi, Shao-Song; Bai, Xiao-Xu; Gu, Li; Bao, Li-Hui; Yang, Hui-Min; An, Wei; Wang, Xiao-Min; Zhang, Hong

2017-07-01

Numerous epidemiological studies suggested that there is a variable cancer risk in patients with Parkinson's disease (PD). However, the underlying mechanisms remain unclear. In the present study, the role of metabotropic glutamate receptor 5 (mGluR5) has been investigated in 6-hydroxydopamine (6-OHDA)-induced PD combined with liver cancer both in vitro and in vivo. We found that PD cellular model from 6-OHDA-lesioned MN9D cells suppressed the growth, migration, and invasion of Hepa1-6 cells via down-regulation of mGluR5-mediated ERK and Akt pathway. The application of 2-methyl-6-(phenylethyl)-pyridine and knockdown of mGluR5 further decreased the effect on Hepa-1-6 cells when co-cultured with conditioned media. The effect was increased by (S)-3,5-dihydroxyphenylglycine and overexpression of mGluR5. Moreover, more release of glutamate from 6-OHDA-lesioned MN9D cells suppressed mGluR5-mediated effect of Hepa1-6 cells. Application of riluzole eliminated the increased glutamate release induced by 6-OHDA in MN9D cells and aggravated the suppressive effect on Hepa-1-6 cells. In addition, the growth of implanted liver cancer was inhibited in 6-OHDA induced PD-like rats, and was associated with increased glutamate release in the serum and down-regulation of mGluR5 in tumor tissue. Collectively, these results indicate that selective antagonism of glutamate and mGluR5 has a potentially beneficial effect in both liver cancer and PD, and thus may provide more understanding for the clinical investigation and further an additional therapeutic target for these two diseases. Copyright © 2017 Elsevier Ltd. All rights reserved.

Multiple quantum filtered 23Na NMR in the Langendorff perfused mouse heart: Ratio of triple/double quantum filtered signals correlates with [Na]i

PubMed Central

Eykyn, Thomas R.; Aksentijević, Dunja; Aughton, Karen L.; Southworth, Richard; Fuller, William; Shattock, Michael J.

2015-01-01

We investigate the potential of multiple quantum filtered (MQF) 23Na NMR to probe intracellular [Na]i in the Langendorff perfused mouse heart. In the presence of Tm(DOTP) shift reagent the triple quantum filtered (TQF) signal originated largely from the intracellular sodium pool with a 32 ± 6% contribution of the total TQF signal arising from extracellular sodium, whilst the rank 2 double-quantum filtered signal (DQF), acquired with a 54.7° flip-angle pulse, originated exclusively from the extracellular sodium pool. Given the different cellular origins of the 23Na MQF signals we propose that the TQF/DQF ratio can be used as a semi-quantitative measure of [Na]i in the mouse heart. We demonstrate a good correlation of this ratio with [Na]i measured with shift reagent at baseline and under conditions of elevated [Na]i. We compare the measurements of [Na]i using both shift reagent and TQF/DQF ratio in a cohort of wild type mouse hearts and in a transgenic PLM3SA mouse expressing a non-phosphorylatable form of phospholemman, showing a modest but measurable elevation of baseline [Na]i. MQF filtered 23Na NMR is a potentially useful tool for studying normal and pathophysiological changes in [Na]i, particularly in transgenic mouse models with altered Na regulation. PMID:26196304

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carter, E.E.

Molten wax shows considerable promise as a fixative and dust control agent in demolition of radioactively contaminated facilities. Sticky molten wax, modified with special surfactants and wetting agents, is capable of not only coating materials but also penetrating into friable or dusty materials and making them incapable of becoming airborne during demolition. Wax also shows significant promise for stabilization of waste residuals that may be contained in buildings undergoing demolition. Some of the building materials that have been tested to date include concrete, wood, sheet-rock, fiber insulation, lime, rock, and paper. Protective clothing, clay, sand, sulfur, and bentonite clay havemore » been tested as surrogates for certain waste materials that may be encountered during building demolition. The paper describes several potential applications of molten wax for dust control in demolition of radioactive contaminated facilities. As a case-study, this paper describes a research test performed for a pipeline closure project being completed by the Idaho Cleanup Project at the Idaho National Laboratory. The project plans to excavate and remove a section of buried Duriron drain piping containing highly radioactive and friable and 'flighty' waste residuals. A full-scale pipeline mockup containing simulated waste was buried in sand to simulate the direct-buried subsurface condition of the subject piping. The pipeline was pre-heated by drawing hot air through the line with a HEPA vacuum blower unit. Molten wax was pumped into the line and allowed to cool. The line was then broken apart in various places to evaluate the permeation performance of the wax. The wax fully permeated all the surrogate materials rendering them non-friable with a consistency similar to modeling clay. Based on the performance during the mockup, it is anticipated that the wax will be highly effective in controlling the spread of radiological contamination during pipe demolition activities. A larger test was completed this year to simulate the work in more realistic conditions. (authors)« less

Predictors of coarse particulate matter and associated endotoxin concentrations in residential environments

NASA Astrophysics Data System (ADS)

Bari, Md. Aynul; MacNeill, Morgan; Kindzierski, Warren B.; Wallace, Lance; Héroux, Marie-Ève; Wheeler, Amanda J.

2014-08-01

Exposure to coarse particulate matter (PM), i.e., particles with an aerodynamic diameter between 2.5 and 10 μm (PM10-2.5), is of increasing interest due to the potential for health effects including asthma, allergy and respiratory symptoms. Limited information is available on indoor and outdoor coarse PM and associated endotoxin exposures. Seven consecutive 24-h samples of indoor and outdoor coarse PM were collected during winter and summer 2010 using Harvard Coarse Impactors in a total of 74 Edmonton homes where no reported smoking took place. Coarse PM filters were subsequently analyzed for endotoxin content. Data were also collected on indoor and outdoor temperature, relative humidity, air exchange rate, housing characteristics and occupants' activities. During winter, outdoor concentrations of coarse PM (median = 6.7 μg/m3, interquartile range, IQR = 3.4-12 μg/m3) were found to be higher than indoor concentrations (median 3.4 μg/m3, IQR = 1.6-5.7 μg/m3); while summer levels of indoor and outdoor concentrations were similar (median 4.5 μg/m3, IQR = 2.3-6.8 μg/m3, and median 4.7 μg/m3, IQR = 2.1-7.9 μg/m3, respectively). Similar predictors were identified for indoor coarse PM in both seasons and included corresponding outdoor coarse PM concentrations, whether vacuuming, sweeping or dusting was performed during the sampling period, and number of occupants in the home. Winter indoor coarse PM predictors also included the number of dogs and indoor endotoxin concentrations. Summer median endotoxin concentrations (indoor: 0.41 EU/m3, outdoor: 0.64 EU/m3) were 4-fold higher than winter concentrations (indoor: 0.12 EU/m3, outdoor: 0.16 EU/m3). Other than outdoor endotoxin concentrations, indoor endotoxin concentration predictors for both seasons were different. Winter endotoxin predictors also included presence of furry pets and whether the vacuum had a high efficiency particulate air (HEPA) filter. Summer endotoxin predictors were problems with mice in the previous 12 months and mean indoor relative humidity levels.

Mercury, monomethyl mercury, and dissolved organic carbon concentrations in surface water entering and exiting constructed wetlands treated with metal-based coagulants, Twitchell Island, California

USGS Publications Warehouse

Stumpner, Elizabeth B.; Kraus, Tamara E.C.; Fleck, Jacob A.; Hansen, Angela M.; Bachand, Sandra M.; Horwath, William R.; DeWild, John F.; Krabbenhoft, David P.; Bachand, Philip A.M.

2015-09-02

Following coagulation, but prior to passage through the wetland cells, coagulation treatments transferred dissolved mercury and carbon to the particulate fraction relative to untreated source water: at the wetland cell inlets, the coagulation treatments decreased concentrations of filtered total mercury by 59–76 percent, filtered monomethyl mercury by 40–70 percent, and dissolved organic carbon by 65–86 percent. Passage through the wetland cells decreased the particulate fraction of mercury in wetland cells that received coagulant-treated water. Changes in total mercury, monomethyl mercury, and dissolved organic carbon concentrations resulting from wetland passage varied both by treatment and season. Despite increased monomethyl mercury in the filtered fraction during wetland passage between March and August, the coagulation-wetland systems generally decreased total mercury (filtered plus particulate) and monomethyl mercury (filtered plus particulate) concentrations relative to source water. Coagulation—either alone or in association with constructed wetlands—could be an effective way to decrease concentrations of mercury and dissolved organic carbon in surface water as well as the bioavailability of mercury in the Sacramento–San Joaquin Delta.

Ultrathin Carbon with Interspersed Graphene/Fullerene-like Nanostructures: A Durable Protective Overcoat for High Density Magnetic Storage

PubMed Central

Dwivedi, Neeraj; Satyanarayana, Nalam; Yeo, Reuben J.; Xu, Hai; Ping Loh, Kian; Tripathy, Sudhiranjan; Bhatia, Charanjit S.

2015-01-01

One of the key issues for future hard disk drive technology is to design and develop ultrathin (<2 nm) overcoats with excellent wear- and corrosion protection and high thermal stability. Forming carbon overcoats (COCs) having interspersed nanostructures by the filtered cathodic vacuum arc (FCVA) process can be an effective approach to achieve the desired target. In this work, by employing a novel bi-level surface modification approach using FCVA, the formation of a high sp3 bonded ultrathin (~1.7 nm) amorphous carbon overcoat with interspersed graphene/fullerene-like nanostructures, grown on magnetic hard disk media, is reported. The in-depth spectroscopic and microscopic analyses by high resolution transmission electron microscopy, scanning tunneling microscopy, time-of-flight secondary ion mass spectrometry, and Raman spectroscopy support the observed findings. Despite a reduction of ~37 % in COC thickness, the FCVA-processed thinner COC (~1.7 nm) shows promising functional performance in terms of lower coefficient of friction (~0.25), higher wear resistance, lower surface energy, excellent hydrophobicity and similar/better oxidation corrosion resistance than current commercial COCs of thickness ~2.7 nm. The surface and tribological properties of FCVA-deposited COC was further improved after deposition of lubricant layer. PMID:26109208

Optimization of a rod pinch diode radiography source at 2.3 MV

NASA Astrophysics Data System (ADS)

Menge, P. R.; Johnson, D. L.; Maenchen, J. E.; Rovang, D. C.; Oliver, B. V.; Rose, D. V.; Welch, D. R.

2003-08-01

Rod pinch diodes have shown considerable capability as high-brightness flash x-ray sources for penetrating dynamic radiography. The rod pinch diode uses a small diameter (0.4-2 mm) anode rod extended through a cathode aperture. When properly configured, the electron beam born off of the aperture edge can self-insulate and pinch onto the tip of the rod creating an intense, small x-ray source. Sandia's SABRE accelerator (2.3 MV, 40 Ω, 70 ns) has been utilized to optimize the source experimentally by maximizing the figure of merit (dose/spot diameter2) and minimizing the diode impedance droop. Many diode parameters have been examined including rod diameter, rod length, rod material, cathode aperture diameter, cathode thickness, power flow gap, vacuum quality, and severity of rod-cathode misalignment. The configuration producing the greatest figure of merit uses a 0.5 mm diameter gold rod, a 6 mm rod extension beyond the cathode aperture (diameter=8 mm), and a 10 cm power flow gap to produce up to 3.5 rad (filtered dose) at 1 m from a 0.85 mm x-ray on-axis spot (1.02 mm at 3° off axis). The resultant survey of parameter space has elucidated several physics issues that are discussed.

Kinetic enhancement via passive deposition of carbon-based nanomaterials in vanadium redox flow batteries

NASA Astrophysics Data System (ADS)

Aaron, Doug; Yeom, Sinchul; Kihm, Kenneth D.; Ashraf Gandomi, Yasser; Ertugrul, Tugrul; Mench, Matthew M.

2017-10-01

Addition of carbon-based nanomaterials to operating flow batteries accomplishes vanadium redox flow battery performance improvement. Initial efforts focus on addition of both pristine graphene and vacuum-filtered reduced graphene oxide (rGO) film on carbon paper supporting electrodes. While the former is unable to withstand convective flow through the porous electrode, the latter shows measurable kinetic improvement, particularly when laid on the polymer electrolyte membrane (PEM) side of the electrode; in contrast to the kinetic performance gain, a deleterious impact on mass transport is observed. Based on this tradeoff, further improvement is realized using perforated rGO films placed on the PEM side of the electrodes. Poor mass transport in the dense rGO film prompts identification of a more uniform, passive deposition method. A suspension of rGO flakes or Vulcan carbon black (XC-72R), both boasting two orders-of-magnitude greater specific surface area than that of common carbon electrodes, is added to the electrolyte reservoirs and allowed to passively deposit on the carbon paper or carbon felt supporting electrodes. For common carbon felt electrodes, addition of rGO flakes or XC-72R enables a tripling of current density at the same 80% voltage efficiency.

Evolution of sp{sup 2} networks with substrate temperature in amorphous carbon films: Experiment and theory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gago, R.; Centro de Micro-Analisis de Materiales, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid; Vinnichenko, M.

2005-07-01

The evolution of sp{sup 2} hybrids in amorphous carbon (a-C) films deposited at different substrate temperatures was studied experimentally and theoretically. The bonding structure of a-C films prepared by filtered cathodic vacuum arc was assessed by the combination of visible Raman spectroscopy, x-ray absorption, and spectroscopic ellipsometry, while a-C structures were generated by molecular-dynamics deposition simulations with the Brenner interatomic potential to determine theoretical sp{sup 2} site distributions. The experimental results show a transition from tetrahedral a-C (ta-C) to sp{sup 2}-rich structures at {approx}500 K. The sp{sup 2} hybrids are mainly arranged in chains or pairs whereas graphitic structures aremore » only promoted for sp{sup 2} fractions above 80%. The theoretical analysis confirms the preferred pairing of isolated sp{sup 2} sites in ta-C, the coalescence of sp{sup 2} clusters for medium sp{sup 2} fractions, and the pronounced formation of rings for sp{sup 2} fractions >80%. However, the dominance of sixfold rings is not reproduced theoretically, probably related to the functional form of the interatomic potential used.« less

Numerical investigation of a helicopter combustion chamber using LES and tabulated chemistry

NASA Astrophysics Data System (ADS)

Auzillon, Pierre; Riber, Eléonore; Gicquel, Laurent Y. M.; Gicquel, Olivier; Darabiha, Nasser; Veynante, Denis; Fiorina, Benoît

2013-01-01

This article presents Large Eddy Simulations (LES) of a realistic aeronautical combustor device: the chamber CTA1 designed by TURBOMECA. Under nominal operating conditions, experiments show hot spots observed on the combustor walls, in the vicinity of the injectors. These high temperature regions disappear when modifying the fuel stream equivalence ratio. In order to account for detailed chemistry effects within LES, the numerical simulation uses the recently developed turbulent combustion model F-TACLES (Filtered TAbulated Chemistry for LES). The principle of this model is first to generate a lookup table where thermochemical variables are computed from a set of filtered laminar unstrained premixed flamelets. To model the interactions between the flame and the turbulence at the subgrid scale, a flame wrinkling analytical model is introduced and the Filtered Density Function (FDF) of the mixture fraction is modeled by a β function. Filtered thermochemical quantities are stored as a function of three coordinates: the filtered progress variable, the filtered mixture fraction and the mixture fraction subgrid scale variance. The chemical lookup table is then coupled with the LES using a mathematical formalism that ensures an accurate prediction of the flame dynamics. The numerical simulation of the CTA1 chamber with the F-TACLES turbulent combustion model reproduces fairly the temperature fields observed in experiments. In particular the influence of the fuel stream equivalence ratio on the flame position is well captured.

Cytotoxic and aryl hydrocarbon hydroxylase-inducing effects of laboratory rodent diets. A cell culture study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Toerroenen, R.; Pelkonen, K.; Kaerenlampi, S.

1991-01-01

Extracts of several rodent diets were studied for their cytotoxic and aryl hydrocarbon hydroxylase-inducing properties by an in vitro method. The cell culture system based on a mouse hepatoma cell line (Hepa-1) was shown to be convenient and sensitive method for screening of diets for these parameters implying the presence of compounds potentially harmful in vivo. Considerable differences among diets and batches were detected. Smallest effects were observed with a semipurified diet and with the unrefined diet which - contrary to other four unrefined diets - contained no fish.

Pilot-scale tests of HEME and HEPA dissolution process

DOE Office of Scientific and Technical Information (OSTI.GOV)

Qureshi, Z.H.; Strege, D.K.

A series of pilot-scale demonstration tests for the dissolution of High Efficiency Mist Eliminators (HEME`s) and High Efficiency Particulate Airfilters (HEPA) were performed on a 1/5th linear scale. These fiberglass filters are to be used in the Defense Waste Processing Facility (DWPF) to decontaminate the effluents from the off-gases generated during the feed preparation process and vitrification. When removed, these filters will be dissolved in the Decontamination Waste Treatment Tank (DWTT) using 5 wt% NaOH solution. The contaminated fiberglass is converted to an aqueous stream which will be transferred to the waste tanks. The filter metal structure will be rinsedmore » with process water before its disposal as low-level solid waste. The pilot-scale study reported here successfully demonstrated a simple one step process using 5 wt% NaOH solution. The proposed process requires the installation of a new water spray ring with 30 nozzles. In addition to the reduced waste generated, the total process time is reduced to 48 hours only (66% saving in time). The pilot-scale tests clearly demonstrated that the dissolution process of HEMEs has two stages - chemical digestion of the filter and mechanical erosion of the digested filter. The digestion is achieved by a boiling 5 wt% caustic solutions, whereas the mechanical break down of the digested filter is successfully achieved by spraying process water on the digested filter. An alternate method of breaking down the digested filter by increased air sparging of the solution was found to be marginally successful are best. The pilot-scale tests also demonstrated that the products of dissolution are easily pumpable by a centrifugal pump.« less

235U Holdup Measurements in Three 321-M Exhaust HEPA Banks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dewberry, R

2005-02-24

The Analytical Development Section of Savannah River National Laboratory (SRNL) was requested by the Facilities Disposition Division to determine the holdup of enriched uranium in the 321-M facility as part of an overall deactivation project of the facility. The 321-M facility was used to fabricate enriched uranium fuel assemblies, lithium-aluminum target tubes, neptunium assemblies, and miscellaneous components for the production reactors. The results of the holdup assays are essential for determining compliance with the Waste Acceptance Criteria, Material Control & Accountability, and to meet criticality safety controls. This report covers holdup measurements of uranium residue in three HEPA filter exhaustmore » banks of the 321-M facility. Each of the exhaust banks has dimensions near 7' x 14' x 4' and represents a complex holdup problem. A portable HPGe detector and EG&G Dart system that contains the high voltage power supply and signal processing electronics were used to determine highly enriched uranium (HEU) holdup. A personal computer with Gamma-Vision software was used to control the Dart MCA and to provide space to store and manipulate multiple 4096-channel {gamma}-ray spectra. Some acquisitions were performed with the portable detector configured to a Canberra Inspector using NDA2000 acquisition and analysis software. Our results for each component uses a mixture of redundant point source and area source acquisitions that yielded HEU contents in the range of 2-10 grams. This report discusses the methodology, non-destructive assay (NDA) measurements, assumptions, and results of the uranium holdup in these items. This report includes use of transmission-corrected assay as well as correction for contributions from secondary area sources.« less

Novel hypothesis to explain why SGLT2 inhibitors inhibit only 30-50% of filtered glucose load in humans.

PubMed

Abdul-Ghani, Muhammad A; DeFronzo, Ralph A; Norton, Luke

2013-10-01

Inhibitors of sodium-glucose cotransporter 2 (SGLT2) are a novel class of antidiabetes drugs, and members of this class are under various stages of clinical development for the management of type 2 diabetes mellitus (T2DM). It is widely accepted that SGLT2 is responsible for >80% of the reabsorption of the renal filtered glucose load. However, maximal doses of SGLT2 inhibitors fail to inhibit >50% of the filtered glucose load. Because the clinical efficacy of this group of drugs is entirely dependent on the amount of glucosuria produced, it is important to understand why SGLT2 inhibitors inhibit <50% of the filtered glucose load. In this Perspective, we provide a novel hypothesis that explains this apparent puzzle and discuss some of the clinical implications inherent in this hypothesis.

Ultrathin Carbon with Interspersed Graphene/Fullerene-like Nanostructures: A Durable Protective Overcoat for High Density Magnetic Storage

NASA Astrophysics Data System (ADS)

Dwivedi, Neeraj; Satyanarayana, Nalam; Yeo, Reuben J.; Xu, Hai; Ping Loh, Kian; Tripathy, Sudhiranjan; Bhatia, Charanjit S.

2015-06-01

One of the key issues for future hard disk drive technology is to design and develop ultrathin (<2 nm) overcoats with excellent wear- and corrosion protection and high thermal stability. Forming carbon overcoats (COCs) having interspersed nanostructures by the filtered cathodic vacuum arc (FCVA) process can be an effective approach to achieve the desired target. In this work, by employing a novel bi-level surface modification approach using FCVA, the formation of a high sp3 bonded ultrathin (~1.7 nm) amorphous carbon overcoat with interspersed graphene/fullerene-like nanostructures, grown on magnetic hard disk media, is reported. The in-depth spectroscopic and microscopic analyses by high resolution transmission electron microscopy, scanning tunneling microscopy, time-of-flight secondary ion mass spectrometry, and Raman spectroscopy support the observed findings. Despite a reduction of ~37 % in COC thickness, the FCVA-processed thinner COC (~1.7 nm) shows promising functional performance in terms of lower coefficient of friction (~0.25), higher wear resistance, lower surface energy, excellent hydrophobicity and similar/better oxidation corrosion resistance than current commercial COCs of thickness ~2.7 nm. The surface and tribological properties of FCVA-deposited COC was further improved after deposition of lubricant layer.

Ultrathin Carbon with Interspersed Graphene/Fullerene-like Nanostructures: A Durable Protective Overcoat for High Density Magnetic Storage.

PubMed

Dwivedi, Neeraj; Satyanarayana, Nalam; Yeo, Reuben J; Xu, Hai; Ping Loh, Kian; Tripathy, Sudhiranjan; Bhatia, Charanjit S

2015-06-25

One of the key issues for future hard disk drive technology is to design and develop ultrathin (<2 nm) overcoats with excellent wear- and corrosion protection and high thermal stability. Forming carbon overcoats (COCs) having interspersed nanostructures by the filtered cathodic vacuum arc (FCVA) process can be an effective approach to achieve the desired target. In this work, by employing a novel bi-level surface modification approach using FCVA, the formation of a high sp(3) bonded ultrathin (~1.7 nm) amorphous carbon overcoat with interspersed graphene/fullerene-like nanostructures, grown on magnetic hard disk media, is reported. The in-depth spectroscopic and microscopic analyses by high resolution transmission electron microscopy, scanning tunneling microscopy, time-of-flight secondary ion mass spectrometry, and Raman spectroscopy support the observed findings. Despite a reduction of ~37% in COC thickness, the FCVA-processed thinner COC (~1.7 nm) shows promising functional performance in terms of lower coefficient of friction (~0.25), higher wear resistance, lower surface energy, excellent hydrophobicity and similar/better oxidation corrosion resistance than current commercial COCs of thickness ~2.7 nm. The surface and tribological properties of FCVA-deposited COC was further improved after deposition of lubricant layer.

Semiautomated Sample Preparation for Protein Stability and Formulation Screening via Buffer Exchange.

PubMed

Ying, William; Levons, Jaquan K; Carney, Andrea; Gandhi, Rajesh; Vydra, Vicky; Rubin, A Erik

2016-06-01

A novel semiautomated buffer exchange process workflow was developed to enable efficient early protein formulation screening. An antibody fragment protein, BMSdab, was used to demonstrate the workflow. The process afforded 60% to 80% cycle time and scientist time savings and significant material efficiencies. These efficiencies ultimately facilitated execution of this stability work earlier in the drug development process, allowing this tool to inform the developability of potential candidates for development from a formulation perspective. To overcome the key technical challenges, the protein solution was buffer-exchanged by centrifuge filtration into formulations for stability screening in a 96-well plate with an ultrafiltration membrane, leveraging automated liquid handling and acoustic volume measurements to allow several cycles of exchanges. The formulations were transferred into a vacuum manifold and sterile filtered into a rack holding 96 glass vials. The vials were sealed with a capmat of individual caps and placed in stability stations. Stability of the samples prepared by this process and by the standard process was demonstrated to be comparable. This process enabled screening a number of formulations of a protein at an early pharmaceutical development stage with a short sample preparation time. © 2015 Society for Laboratory Automation and Screening.

FILTERED WATER RESERVOIR, LOOKING NORTHWEST. A CORNER OF THE NEW ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

FILTERED WATER RESERVOIR, LOOKING NORTHWEST. A CORNER OF THE NEW PUMPING STATION IS VISIBLE AT RIGHT. - Division Avenue Pumping Station & Filtration Plant, West 45th Street and Division Avenue, Cleveland, Cuyahoga County, OH

Spatially pooled depth-dependent reservoir storage, elevation, and water-quality data for selected reservoirs in Texas, January 1965-January 2010

USGS Publications Warehouse

Burley, Thomas E.; Asquith, William H.; Brooks, Donald L.

2011-01-01

The U.S. Geological Survey (USGS), in cooperation with Texas Tech University, constructed a dataset of selected reservoir storage (daily and instantaneous values), reservoir elevation (daily and instantaneous values), and water-quality data from 59 reservoirs throughout Texas. The period of record for the data is as large as January 1965-January 2010. Data were acquired from existing databases, spreadsheets, delimited text files, and hard-copy reports. The goal was to obtain as much data as possible; therefore, no data acquisition restrictions specifying a particular time window were used. Primary data sources include the USGS National Water Information System, the Texas Commission on Environmental Quality Surface Water-Quality Management Information System, and the Texas Water Development Board monthly Texas Water Condition Reports. Additional water-quality data for six reservoirs were obtained from USGS Texas Annual Water Data Reports. Data were combined from the multiple sources to create as complete a set of properties and constituents as the disparate databases allowed. By devising a unique per-reservoir short name to represent all sites on a reservoir regardless of their source, all sampling sites at a reservoir were spatially pooled by reservoir and temporally combined by date. Reservoir selection was based on various criteria including the availability of water-quality properties and constituents that might affect the trophic status of the reservoir and could also be important for understanding possible effects of climate change in the future. Other considerations in the selection of reservoirs included the general reservoir-specific period of record, the availability of concurrent reservoir storage or elevation data to match with water-quality data, and the availability of sample depth measurements. Additional separate selection criteria included historic information pertaining to blooms of golden algae. Physical properties and constituents were water temperature, reservoir storage, reservoir elevation, specific conductance, dissolved oxygen, pH, unfiltered salinity, unfiltered total nitrogen, filtered total nitrogen, unfiltered nitrate plus nitrite, unfiltered phosphorus, filtered phosphorus, unfiltered carbon, carbon in suspended sediment, total hardness, unfiltered noncarbonate hardness, filtered noncarbonate hardness, unfiltered calcium, filtered calcium, unfiltered magnesium, filtered magnesium, unfiltered sodium, filtered sodium, unfiltered potassium, filtered potassium, filtered chloride, filtered sulfate, unfiltered fluoride, and filtered fluoride. When possible, USGS and Texas Commission on Environmental Quality water-quality properties and constituents were matched using the database parameter codes for individual physical properties and constituents, descriptions of each physical property or constituent, and their reporting units. This report presents a collection of delimited text files of source-aggregated, spatially pooled, depth-dependent, instantaneous water-quality data as well as instantaneous, daily, and monthly storage and elevation reservoir data.

The HARP domain dictates the annealing helicase activity of HARP/SMARCAL1.

PubMed

Ghosal, Gargi; Yuan, Jingsong; Chen, Junjie

2011-06-01

Mutations in HepA-related protein (HARP, or SMARCAL1) cause Schimke immunoosseous dysplasia (SIOD). HARP has ATP-dependent annealing helicase activity, which helps to stabilize stalled replication forks and facilitate DNA repair during replication. Here, we show that the conserved tandem HARP (2HP) domain dictates this annealing helicase activity. Furthermore, chimeric proteins generated by fusing the 2HP domain of HARP with the SNF2 domain of BRG1 or HELLS show annealing helicase activity in vitro and, when targeted to replication forks, mimic the functions of HARP in vivo. We propose that the HARP domain endows HARP with this ATP-driven annealing helicase activity.

Optical air data systems and methods

NASA Technical Reports Server (NTRS)

Caldwell, Loren M. (Inventor); Tang, Shoou-Yu (Inventor); O'Brien, Martin J. (Inventor)

2009-01-01

A method for remotely sensing air outside a moving aircraft includes generating laser radiation within a swept frequency range. A portion of the laser radiation is projected from the aircraft into the air to induce scattered laser radiation. Filtered scattered laser radiation, filtered laser radiation, and unfiltered laser radiation are detected. At least one actual ratio is determined from data corresponding to the filtered scattered laser radiation and the unfiltered laser radiation. One or more air parameters are determined by correlating the actual ratio to at least one reference ratio.

Development of working hypotheses linking management of the Missouri River to population dynamics of Scaphirhynchus albus (pallid sturgeon)

USGS Publications Warehouse

Jacobson, Robert B.; Parsley, Michael J.; Annis, Mandy L.; Colvin, Michael E.; Welker, Timothy L.; James, Daniel A.

2016-01-20

The initial set of candidate hypotheses provides a useful starting point for quantitative modeling and adaptive management of the river and species. We anticipate that hypotheses will change from the set of working management hypotheses as adaptive management progresses. More importantly, hypotheses that have been filtered out of our multistep process are still being considered. These filtered hypotheses are archived and if existing hypotheses are determined to be inadequate to explain observed population dynamics, new hypotheses can be created or filtered hypotheses can be reinstated.

Method for dissolution and stabilization of silica-rich fibers

DOEpatents

Jantzen, Carol M.

1997-01-01

A method for dissolving silica-rich fibers such as borosilicate fibers, fiberglass and asbestos to stabilize them for disposal. The method comprises (1) immersing the fibers in hot, five-weight-percent sodium hydroxide solution until the concentration of dissolved silica reaches equilibrium and a only a residue is left (about 48 hours), then immersing the residue in hot, five-weight-percent nitric acid until the residue dissolves (about 96 hours). After adjusting the pH of the dissolved fibers to be caustic, the solution can then be added to a waste vitrification stream for safe disposal. The method is useful in disposing contaminated HEME and HEPA filters.

Mass Spectrometric Characteristics of Prenylated Indole Derivatives from Marine-Derived Penicillium sp. NH-SL.

PubMed

Ding, Hui; Ding, Wanjing; Ma, Zhongjun

2017-03-22

Two prenylated indole alkaloids were isolated from the ethyl acetate extracts of a marine-derived fungus Penicillium sp. NH-SL and one of them exhibited potent cytotoxic activity against mouse hepa 1c1c7 cells. In order to detect other bioactive analogs, we used liquid chromatogram tandem mass spectrometry (LC-MS/MS) to analyze the mass spectrometric characteristics of the isolated compounds as well as the crude extracts. As a result, three other analogs were detected, and their structures were deduced according to the similar fragmentation patterns. This is the first systematic report on the mass spectrometric characteristics of prenylated indole derivatives.

Novel Hypothesis to Explain Why SGLT2 Inhibitors Inhibit Only 30–50% of Filtered Glucose Load in Humans

PubMed Central

Abdul-Ghani, Muhammad A.; DeFronzo, Ralph A.; Norton, Luke

2013-01-01

Inhibitors of sodium-glucose cotransporter 2 (SGLT2) are a novel class of antidiabetes drugs, and members of this class are under various stages of clinical development for the management of type 2 diabetes mellitus (T2DM). It is widely accepted that SGLT2 is responsible for >80% of the reabsorption of the renal filtered glucose load. However, maximal doses of SGLT2 inhibitors fail to inhibit >50% of the filtered glucose load. Because the clinical efficacy of this group of drugs is entirely dependent on the amount of glucosuria produced, it is important to understand why SGLT2 inhibitors inhibit <50% of the filtered glucose load. In this Perspective, we provide a novel hypothesis that explains this apparent puzzle and discuss some of the clinical implications inherent in this hypothesis. PMID:24065789

Optical air data systems and methods

NASA Technical Reports Server (NTRS)

Caldwell, Loren M. (Inventor); O'Brien, Martin J. (Inventor); Tang, Shoou-Yu (Inventor)

2011-01-01

A method for remotely sensing air outside a moving aircraft includes generating laser radiation within a swept frequency range. A portion of the laser radiation is projected from the aircraft into the air to induce scattered laser radiation. Filtered scattered laser radiation, filtered laser radiation, and unfiltered laser radiation are detected. At least one actual ratio is determined from data corresponding to the filtered scattered laser radiation and the unfiltered laser radiation. One or more air parameters are determined by correlating the actual ratio to at least one reference ratio.

Effects of spatial frequency content on classification of face gender and expression.

PubMed

Aguado, Luis; Serrano-Pedraza, Ignacio; Rodríguez, Sonia; Román, Francisco J

2010-11-01

The role of different spatial frequency bands on face gender and expression categorization was studied in three experiments. Accuracy and reaction time were measured for unfiltered, low-pass (cut-off frequency of 1 cycle/deg) and high-pass (cutoff frequency of 3 cycles/deg) filtered faces. Filtered and unfiltered faces were equated in root-mean-squared contrast. For low-pass filtered faces reaction times were higher than unfiltered and high-pass filtered faces in both categorization tasks. In the expression task, these results were obtained with expressive faces presented in isolation (Experiment 1) and also with neutral-expressive dynamic sequences where each expressive face was preceded by a briefly presented neutral version of the same face (Experiment 2). For high-pass filtered faces different effects were observed on gender and expression categorization. While both speed and accuracy of gender categorization were reduced comparing to unfiltered faces, the efficiency of expression classification remained similar. Finally, we found no differences between expressive and non expressive faces in the effects of spatial frequency filtering on gender categorization (Experiment 3). These results show a common role of information from the high spatial frequency band in the categorization of face gender and expression.

Fuzzy Finite-Time Command Filtered Control of Nonlinear Systems With Input Saturation.

PubMed

Yu, Jinpeng; Zhao, Lin; Yu, Haisheng; Lin, Chong; Dong, Wenjie

2017-08-22

This paper considers the fuzzy finite-time tracking control problem for a class of nonlinear systems with input saturation. A novel fuzzy finite-time command filtered backstepping approach is proposed by introducing the fuzzy finite-time command filter, designing the new virtual control signals and the modified error compensation signals. The proposed approach not only holds the advantages of the conventional command-filtered backstepping control, but also guarantees the finite-time convergence. A practical example is included to show the effectiveness of the proposed method.

IMMUNOBLOT ANALYSIS OF PROTEINS ASSOCIATED WITH SELF-ASSEMBLED MONOLAYER SURFACES OF DEFINED CHEMISTRIES

PubMed Central

Cornelius, Rena M.; Shankar, Sucharita P.; Brash, John L.; Babensee, Julia E.

2011-01-01

Intact and fragmented proteins, eluted from self assembled monolayer (SAM) surfaces of alkanethiols of different chemistries (-CH3, -OH, -COOH, -NH2 ), following exposure to human plasma (HP) or human serum (HS), were examined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting techniques. The SAM surfaces were incubated for 1 hour with 10% (v/v) sterile-filtered heat-inactivated (h.i.) HS or 1% (v/v) sterile-filtered h.i. HP preparations [both in phosphate buffered saline (PBS)]. Adsorbed proteins were eluted using 10% SDS/2.3% dithioerythritol for characterization of protein profiles. The type of incubating medium may be an important determinant of adsorbed protein profiles, since some variations were observed in eluates from filtered versus control unfiltered h.i. 10% HS or 1% HP. Albumin and apolipoprotein A1 were consistently detected in both filtered h.i 10% HS and 1% HP eluates from all SAM surfaces and from control tissue culture-treated polystyrene (TCPS). Interestingly, Factor H and Factor I, antithrombin, prothrombin, high molecular weight kininogen (HMWK) and IgG were present in eluates from OH, COOH and NH2 SAM surfaces and in eluates from TCPS, but not in eluates from CH3 SAM surfaces, following exposure to filtered h.i. 10% HS. These results suggest that CH3 SAM surfaces were the least pro-inflammatory of all SAM surfaces. Overall, similar trends were observed in the profiles of proteins eluted from surfaces exposed to filtered 10% HS or 1% HP. However the unique profiles of adsorbed proteins on different SAM surface chemistries may be related to their differential interactions with cells, including immune/inflammatory cells. PMID:21509932

Preparation and physicochemical properties of whole-bean soymilk.

PubMed

Kuo, Hsin-Yu; Chen, Shih-Hsin; Yeh, An-I

2014-01-22

Whole-bean soymilk has been prepared by using media-milling. Some characteristics of media-milled soymilk have been determined and compared with filtered soymilk (similar to commercial ones) and whole-bean soymilk prepared by blending. There existed particles in the nano/submicrometer scale in both media-milled and filtered soymilk. The particles in blended soymilk were greater than 1 μm. Media-milled soymilk was the most stable among three samples, even after autoclaving. Solid recovery (98.44 ± 0.16%), viscosity (160.59 ± 4.26 cps), dietary fiber (22.68 ± 0.97% on dry basis), total polyphenol recovery (95.15 ± 7.09%), and isoflavone content (4.42 ± 0.03 mg/g dry solid) of media-milled samples were greater than those of filtered ones. Aglycones, the most bioactive form of isoflavone, in autoclaved media-milled soymilk were more than 2-fold those in autoclaved filtered soymilk. With almost no okara generated, the media-milled soymilk retained fiber in soybeans which would be beneficial to human health.

Silicon etch with chromium ions generated by a filtered or non-filtered cathodic arc discharge

PubMed Central

Scopece, Daniele; Döbeli, Max; Passerone, Daniele; Maeder, Xavier; Neels, Antonia; Widrig, Beno; Dommann, Alex; Müller, Ulrich; Ramm, Jürgen

2016-01-01

Abstract The pre-treatment of substrate surfaces prior to deposition is important for the adhesion of physical vapour deposition coatings. This work investigates Si surfaces after the bombardment by energetic Cr ions which are created in cathodic arc discharges. The effect of the pre-treatment is analysed by X-ray diffraction, Rutherford backscattering spectroscopy, scanning electron microscopy and in-depth X-ray photoemission spectroscopy and compared for Cr vapour produced from a filtered and non-filtered cathodic arc discharge. Cr coverage as a function of ion energy was also predicted by TRIDYN Monte Carlo calculations. Discrepancies between measured and simulated values in the transition regime between layer growth and surface removal can be explained by the chemical reactions between Cr ions and the Si substrate or between the substrate surface and the residual gases. Simulations help to find optimum and more stable parameters for specific film and substrate combinations faster than trial-and-error procedure. PMID:27877854

Leaf photosynthetic and water-relations responses for 'Valencia' orange trees exposed to oxidant air pollution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olszyk, D.M.; Takemoto, B.K.; Poe, M.

1991-01-01

Leaf responses were measured to test a hypothesis that reduced photosynthetic capacity and/or altered water relations were associated with reductions in yield for 'Valencia' orange trees (Citrus sinensis (L.), Osbeck) exposed to ambient oxidant air pollution. Exposures were continuous for 4 years to three levels of oxidants (in charcoal-filtered, half-filtered, and non-filtered air). Oxidants had no effect on net leaf photosynthetic rates or on photosynthetic pigment concentrations. A single set of measurements indicated that oxidants increased leaf starch concentrations (24%) prior to flowering, suggesting a change in photosynthate allocation. Leaves exposed to oxidants had small, but consistent, changes in watermore » relations over the summer growing season, compared to trees growing in filtered air. Other changes included decreased stomatal conductance (12%) and transpiration (9%) rates, and increased water pressure potentials (5%). While all responses were subtle, their cumulative impact over 4 years indicated that 'Valencia' orange trees were subject to increased ambient oxidant stress.« less

Erythemal irradiances of filtered ultraviolet radiation

NASA Astrophysics Data System (ADS)

Parisi, A. V.; Wong, J. C. F.

1997-07-01

A spectrum evaluator employing four passive dosimeters has been used to evaluate the time averaged spectrum to allow calculation of the erythemal exposures resulting from the predominantly UVA component of filtered solar ultraviolet radiation. An exposure interval of approximately 20 min to autumn and spring sunshine was required for the spectrum evaluator to allow evaluation of the filtered source spectrum. For a clear spring day an erythemal exposure of 0.85 MED (minimum erythemal dose) to a horizontal plane and 0.38 MED to a vertical plane over a 6 h period was measured within a glass enclosure. For a partially cloudy day six weeks later, these were 0.89 and 0.44 MED for the horizontal and the vertical planes respectively. The ratios of the filtered to the unfiltered erythemal exposures within and outside the enclosure respectively ranged from 0.08 to 0.18 throughout the two days.

Endoplasmic Reticulum Stress in Mice Increases Hepatic Expression of Genes Carrying a Premature Termination Codon via a Nutritional Status-Independent GRP78-Dependent Mechanism.

PubMed

Harada, Nagakatsu; Okuyama, Maiko; Yoshikatsu, Aya; Yamamoto, Hironori; Ishiwata, Saori; Hamada, Chikako; Hirose, Tomoyo; Shono, Masayuki; Kuroda, Masashi; Tsutsumi, Rie; Takeo, Jiro; Taketani, Yutaka; Nakaya, Yutaka; Sakaue, Hiroshi

2017-11-01

Nonsense-mediated mRNA decay (NMD) degrades mRNAs carrying a premature termination codon (PTC) in eukaryotes. Cellular stresses, including endoplasmic reticulum (ER) stress, inhibit NMD, and up-regulate PTC-containing mRNA (PTC-mRNA) levels in several cell lines. However, whether similar effects exist under in vivo conditions that involve systemic nutritional status is unclear. Here, we compared the effects of pharmacological induction of ER stress with those of nutritional interventions on hepatic PTC-mRNA levels in mice. In mouse livers, the ER stress inducer tunicamycin increased PTC-mRNA levels of endogenous marker genes. Tunicamycin decreased body weight and perturbed nutrient metabolism in mice. Food restriction or deprivation mimicked the effect of tunicamycin on weight loss and metabolism, but did not increase PTC-mRNA levels. Hyperphagia-induced obesity also had little effect on hepatic PTC-mRNA levels. Meanwhile, in mouse liver phosphorylation of eIF2α, a factor that regulates NMD, was increased by both tunicamycin and nutritional interventions. Hepatic expression of GRP78, a central chaperone in ER stress responses, was increased by tunicamycin but not by the nutritional interventions. In cultured liver cells (Hepa), exogenous overexpression of a phosphomimetic eIF2α failed to increase PTC-mRNA levels. However, GRP78 overexpression in Hepa cells increased PTC-mRNA and PTC-mRNA-derived protein levels. ER stress promoted localization of GRP78 to mitochondria, and exogenous expression of a GRP78 fusion protein targeted to mitochondria mimicked the effect of wild type GRP78. These results indicate that GRP78, but not nutritional status, is a potent up-regulator of hepatic PTC-mRNA levels during induction of ER stress in vivo. J. Cell. Biochem. 118: 3810-3824, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

E4bp4 regulates carboxylesterase 2 enzymes through repression of the nuclear receptor Rev-erbα in mice.

PubMed

Zhao, Mengjing; Zhang, Tianpeng; Yu, Fangjun; Guo, Lianxia; Wu, Baojian

2018-06-01

Carboxylesterases (CES) are a family of phase I enzymes that play an important role in xenobiotic clearance and lipid metabolism. Here, we investigate a potential role of E4 promoter-binding protein 4 (E4bp4) in regulation of Ces and CPT-11 (irinotecan, a first-line drug for treating colorectal cancer) pharmacokinetics in mice. Mouse hepatoma Hepa-1c1c7 cells were transfected with Rev-erbα expression plasmid or siRNA targeting E4bp4. The relative mRNA and protein levels of Ces enzymes in the cells or the livers of wild-type and E4bp4-deficient (E4bp4 -/- ) mice were determined by qPCR and Western blotting, respectively. Transcriptional regulation of Ces by E4bp4/Rev-erbα were investigated using luciferase reporter, mobility shift, and co-immunoprecipitation (Co-IP) assays. Pharmacokinetic studies were performed with wild-type and E4bp4 -/- mice after intraperitoneal injection of CPT-11. E4bp4 ablation down-regulated an array of hepatic Ces genes in mice. E4bp4 -/- mice also showed reduced Ces-mediated metabolism and elevated systemic exposure of CPT-11, a well-known Ces substrate. Consistently, E4bp4 knockdown reduced the expression of Ces genes (Ces2b, Ces2e and Ces2f) in Hepa-1c1c7 cells. Furthermore, Rev-erbα repressed the transcription of Ces2b, whereas E4bp4 antagonized this repressive action. Co-IP experiment confirmed a direct interaction between E4bp4 and Rev-erbα. Through a combination of promoter analysis and mobility shift assays, we demonstrated that Rev-erbα trans-repressed Ces (Ces2b) through its specific binding to the -767 to-754 bp promoter region. In conclusion, E4bp4 regulates Ces enzymes through inhibition of the transrepression activity of Rev-erbα, thereby impacting the metabolism and pharmacokinetics of Ces substrates. Copyright © 2018 Elsevier Inc. All rights reserved.

ANALYSIS OF VAPORS FROM METHYLENE CHLORIDE EXTRACTS OF NUCLEAR GRADE HEPA FILTER FIBERGLASS SAMPLES

DOE Office of Scientific and Technical Information (OSTI.GOV)

FRYE JM; ANASTOS HL; GUTIERREZ FC

2012-06-07

While several organic compounds were detected in the vapor samples used in the reenactment of the preparation of mounts from the extracts of nuclear grade high-efficiency particulate air filter fiberglass samples, the most significant species present in the samples were methylene chloride, phenol, phenol-d6, and 2-fluorophenol. These species were all known to be present in the extracts, but were expected to have evaporated during the preparation of the mounts, as the mounts appeared to be dry before any vapor was collected. These species were present at the following percentages of their respective occupational exposure limits: methylene chloride, 2%; phenol, 0.4%;more » and phenol-d6, 0.6%. However, there is no established limit for 2-fluorophenol. Several other compounds were detected at low levels for which, as in the case of 2-fluorophenol, there are no established permissible exposure limits. These compounds include 2-chlorophenol; N-nitroso-1-propanamine; 2-fluoro-1,1{prime}-biphenyl; 1,2-dihydroacenaphthylene; 2,5-cyclohexadiene-1,4-dione,2,6-bis(1,1-dimethylethyl); trimethyl oxirane; n-propylpropanamine; 2-(Propylamino)ethanol; 4-methoxy-1-butene; 6-methyl-5-hepten-2-one; and 3,4-dimethylpyridine. Some of these were among those added as surrogates or spike standards as part ofthe Advanced Technologies and Laboratories International, Inc. preparation ofthe extract of the HEPA filter media and are indicated as such in the data tables in Section 2, Results; other compounds found were not previously known to be present. The main inorganic species detected (sulfate, sodium, and sulfur) are also consistent with species added in the preparation of the methylene chloride extract of the high-efficiency particulate air sample.« less

Integrating research evidence and physical activity policy making-REPOPA project.

PubMed

Aro, Arja R; Bertram, Maja; Hämäläinen, Riitta-Maija; Van De Goor, Ien; Skovgaard, Thomas; Valente, Adriana; Castellani, Tommaso; Chereches, Razvan; Edwards, Nancy

2016-06-01

Evidence shows that regular physical activity is enhanced by supporting environment. Studies are needed to integrate research evidence into health enhancing, cross-sector physical activity (HEPA) policy making. This article presents the rationale, study design, measurement procedures and the initial results of the first phase of six European countries in a five-year research project (2011-2016), REsearch into POlicy to enhance Physical Activity (REPOPA). REPOPA is programmatic research; it consists of linked studies; the first phase studied the use of evidence in 21 policies in implementation to learn more in depth from the policy making process and carried out 86 qualitative stakeholder interviews. The second, ongoing phase builds on the central findings of the first phase in each country; it consists of two sets of interventions: game simulations to study cross-sector collaboration and organizational change processes in the use of evidence and locally tailored interventions to increase knowledge integration. The results of the first two study phases will be tested and validated among policy makers and other stakeholders in the third phase using a Delphi process. Initial results from the first project phase showed the lack of explicit evidence use in HEPA policy making. Facilitators and barriers of the evidence use were the availability of institutional resources and support but also networking between researchers and policy makers. REPOPA will increase understanding use of research evidence in different contexts; develop guidance and tools and establish sustainable structures such as networks and platforms between academics and policy makers across relevant sectors. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Down-regulation of the detoxifying enzyme NAD(P)H:quinone oxidoreductase 1 by vanadium in Hepa 1c1c7 cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anwar-Mohamed, Anwar; El-Kadi, Ayman O.S.

2009-05-01

Recent data suggest that vanadium (V{sup 5+}) compounds exert protective effects against chemical-induced carcinogenesis, mainly through modifying various xenobiotic metabolizing enzymes. In fact, we have shown that V{sup 5+} down-regulates the expression of Cyp1a1 at the transcriptional level through an ATP-dependent mechanism. However, incongruously, there is increasing evidence that V{sup 5+} is found in higher amounts in cancer cells and tissues than in normal cells or tissues. Therefore, the current study aims to address the possible effect of this metal on the regulation of expression of an enzyme that helps maintain endogenous antioxidants used to protect tissues/cells from mutagens, carcinogens,more » and oxidative stress damage, NAD(P)H:quinone oxidoreductase 1 (Nqo1). In an attempt to examine these effects, Hepa 1c1c7 cells and its AhR-deficient version, c12, were treated with increasing concentrations of V{sup 5+} in the presence of two distinct Nqo1 inducers, the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and isothiocyanate sulforaphane (SUL). Our results showed that V{sup 5+} inhibits the TCDD- and SUL-mediated induction of Nqo1 at mRNA, protein, and catalytic activity levels. At transcriptional level, V{sup 5+} was able to decrease the TCDD- and SUL-induced nuclear accumulation of Nrf2 and the subsequent binding to antioxidant responsive element (ARE) without affecting Nrf2 protein levels. Looking at post-transcriptional level; we found that V{sup 5+} did not affect Nqo1 mRNA transcripts turn-over rates. However, at the post-translational level V{sup 5+} increased Nqo1 protein half-life. In conclusion, the present study demonstrates that V{sup 5+} down-regulates Nqo1 at the transcriptional level, possibly through inhibiting the ATP-dependent activation of Nrf2.« less

METAL MEDIA FILTERS, AG-1 SECTION FI

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adamson, D.

One application of metal media filters is in various nuclear air cleaning processes including applications for protecting workers, the public and the environment from hazardous and radioactive particles. To support this application the development of the ASME AG-1 FI Standard on Metal Media has been under way for more than ten years. Development of the proposed section has required resolving several difficult issues associated with operating conditions (media velocity, pressure drop, etc.), qualification testing, and quality acceptance testing. Performance characteristics of metal media are dramatically different than the glass fiber media with respect to parameters like differential pressures, operating temperatures,more » media strength, etc. These differences make existing data for a glass fiber media inadequate for qualifying a metal media filter for AG-1. In the past much work has been conducted on metal media filters at facilities such as Lawrence Livermore National Laboratory (LLNL) and Savannah River National Laboratory (SRNL) to qualify the media as High Efficiency Particulate Air (HEPA) Filters. Particle retention testing has been conducted at Oak Ridge Filter Test Facility and at Air Techniques International (ATI) to prove that the metal media meets or exceeds the 99.97% particle retention required for a HEPA Filter. Even with his testing, data was lacking to complete an AG-1 FI Standard on metal media. With funding secured by Mississippi State University (MSU) from National Nuclear Security Administration (NNSA), a research test stand is being designed and fabricated at MSU's Institute for Clean Energy Technology (ICET) Facility to obtain qualification data on metal media. This in turn will support required data needed for the FI Standard. The paper will discuss in detail how the test stand at MSU will obtain the necessary data to complete the FI Standard.« less

Upregulated expression of La ribonucleoprotein domain family member 6 and collagen type I gene following water-filtered broad-spectrum near-infrared irradiation in a 3-dimensional human epidermal tissue culture model as revealed by microarray analysis.

PubMed

Tanaka, Yohei; Nakayama, Jun

2018-05-01

Water-filtered broad-spectrum near-infrared irradiation can induce various biological effects, as our previous clinical, histological, and biochemical investigations have shown. However, few studies that examined the changes thus induced in gene expression. The aim was to investigate the changes in gene expression in a 3-dimensional reconstructed epidermal tissue culture exposed to water-filtered broad-spectrum near-infrared irradiation. DNA microarray and quantitative real-time polymerase chain reaction (PCR) analysis was used to assess gene expression levels in a 3-dimensional reconstructed epidermal model composed of normal human epidermal cells exposed to water-filtered broad-spectrum near-infrared irradiation. The water filter allowed 1000-1800 nm wavelengths and excluded 1400-1500 nm wavelengths, and cells were exposed to 5 or 10 rounds of near-infrared irradiation at 10 J/cm 2 . A DNA microarray with over 50 000 different probes showed 18 genes that were upregulated or downregulated by at least twofold after irradiation. Quantitative real-time PCR revealed that, relative to control cells, the gene encoding La ribonucleoprotein domain family member 6 (LARP6), which regulates collagen expression, was significantly and dose-dependently upregulated (P < 0.05) by water-filtered broad-spectrum near-infrared exposure. Gene encoding transcripts of collagen type I were significantly upregulated compared with controls (P < 0.05). This study demonstrates the ability of water-filtered broad-spectrum near-infrared irradiation to stimulate the production of type I collagen. © 2017 The Australasian College of Dermatologists.

Phototherapy with blue and green mixed-light is as effective against unconjugated jaundice as blue light and reduces oxidative stress in the Gunn rat model.

PubMed

Uchida, Yumiko; Morimoto, Yukihiro; Uchiike, Takao; Kamamoto, Tomoyuki; Hayashi, Tamaki; Arai, Ikuyo; Nishikubo, Toshiya; Takahashi, Yukihiro

2015-07-01

Phototherapy using blue light-emitting diodes (LED) is effective against neonatal jaundice. However, green light phototherapy also reduces unconjugated jaundice. We aimed to determine whether mixed blue and green light can relieve jaundice with minimal oxidative stress as effectively as either blue or green light alone in a rat model. Gunn rats were exposed to phototherapy with blue (420-520 nm), filtered blue (FB; 440-520 nm without<440-nm wavelengths, FB50 (half the irradiance of filtered blue), mixed (filtered 50% blue and 50% green), and green (490-590 nm) LED irradiation for 24h. The effects of phototherapy are expressed as ratios of serum total (TB) and unbound (UB) bilirubin before and after exposure to each LED. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was measured by HPLC before and after exposure to each LED to determine photo-oxidative stress. Values < 1.00 indicate effective phototherapy. The ratios of TB and UB were decreased to 0.85, 0.89, 1.07, 0.90, and 1.04, and 0.85, 0.94, 0.93, 0.89, and 1.09 after exposure to blue, filtered blue, FB50, and filtered blue mixed with green LED, respectively. In contrast, urinary 8-OHdG increased to 2.03, 1.25, 0.96, 1.36, 1.31, and 1.23 after exposure to blue, filtered blue, FB50, mixed, green LED, and control, indicating side-effects (> 1.00), respectively. Blue plus green phototherapy is as effective as blue phototherapy and it attenuates irradiation-induced oxidative stress. Combined blue and green spectra might be effective against neonatal hyperbilirubinemia. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Dispersion quality of amine functionalized multiwall carbon nanotubes plays critical roles in polymerase chain reaction enhancement

NASA Astrophysics Data System (ADS)

Yuce, Meral; Budak, Hikmet

2014-12-01

Impact of dispersion quality of NH2-MWCNTs (13-18 nm in diameter with a length between 1 and 12 µm, >99 % purity) in the amplification efficiency of a random DNA oligonucleotide library (96 bp) was investigated. Amplification yield in the presence of non-filtered NH2-MWCNT dispersion, filtered NH2-MWCNT dispersion and surface-attached NH2-MWCNTs was explored, and physical interactions between NH2-MWCNTs and major PCR reagents including DNA template, wild type Taq DNA polymerase enzyme and primers were determined using high resolution polyacrylamide gel electrophoresis, dynamic light scattering, UV-Vis-NIR spectroscopy and scanning electron microscopy techniques. The results revealed that presence of NH2-MWCNT dispersion which was sonicated, centrifuged and filtered, enhanced the total PCR efficiency up to 70 % while the presence of NH2-MWCNT only centrifuged after sonication, inhibited the reaction significantly at similar concentrations. Furthermore, the NH2-MWCNTs coupled covalently onto magnetic microspheres, contributed for the specificity enhancement whilst decreasing the amplification efficiency by 30 % at the maximum concentration, which suggests a removable enhancement system for sensitive applications. On the other hand, the relative hydrodynamic size distribution measurements displayed a clear difference between the filtered NH2 and non-filtered NH2-MWCNT water dispersions, which justifies the inhibition of the amplification by the non-filtered NH2-MWCNTs containing big agglomerates and bundles. Finally, we demonstrated that major PCR components adsorb onto the NH2-MWCNTs with diverse affinities, and maintain their functions after adsorption, which provides a good framework to further develop tunable NH2-MWCNT-carriers to be utilized in various nanobiotechnology and material science applications.

Importance of monsoon rainfall in mass fluxes of filtered and unfiltered mercury in Gwangyang Bay, Korea.

PubMed

Jang, Jiyi; Han, Seunghee

2011-03-15

We investigated the effects of the East Asian Summer Monsoon (EASM), which brings approximately half of Korea's annual rainfall in July, on the concentration and particle-water partitioning, and sources of Hg in coastal waters. Surface seawater samples were collected from eight sites in Gwangyang Bay, Korea, during the monsoon (July, 2009) and non-monsoon dry (April and November, 2009) seasons and the concentrations of suspended particulate matter, chlorophyll-a, and unfiltered and filtered Hg were determined. We found significant (p<0.05) increases in filtered Hg in the monsoon season (1.8 ± 0.019 pM) compared to the dry season (0.62 ± 0.047 pM). In contrast, the Hg concentrations associated with particles showed no significant differences (p>0.05) between the monsoon (459 ± 141 pmol g(-1)) and the dry season (346 ± 30 pmol g(-1)), which resulted in decreased particle-water partition coefficients of Hg in the monsoon season compared to the values in the dry season: 5.7 ± 0.1 in April, 5.3 ± 0.1 in July, and 5.8 ± 0.1 in November. The annual Hg input to Gwangyang Bay was estimated at 64 ± 6.6 mol yr(-1) and 27 ± 1.9 mol yr(-1) for unfiltered and filtered Hg, respectively. The Hg discharged from rivers was a major source of Hg in Gwangyang Bay: the river input contributed 83 ± 13% of total input of unfiltered and 73 ± 6.0% of filtered Hg. On a monthly basis, unfiltered Hg input was 17 ± 11 mol month(-1) in the monsoon season and 3.2 ± 0.70 mol month(-1) in the dry season, while filtered Hg input was 7.1 ± 4.1 mol month(-1) in the monsoon and 1.3 ± 0.26 mol month(-1) in the dry. Consequently, the EASM resulted in an unfiltered Hg input 5.3 times greater than the mean dry month input and a filtered Hg input 5.5 times greater than the mean dry month input, which is mainly attributable to enhanced river water discharge during the monsoon season. Copyright © 2011 Elsevier B.V. All rights reserved.

Advancements to the planogram frequency–distance rebinning algorithm

PubMed Central

Champley, Kyle M; Raylman, Raymond R; Kinahan, Paul E

2010-01-01

In this paper we consider the task of image reconstruction in positron emission tomography (PET) with the planogram frequency–distance rebinning (PFDR) algorithm. The PFDR algorithm is a rebinning algorithm for PET systems with panel detectors. The algorithm is derived in the planogram coordinate system which is a native data format for PET systems with panel detectors. A rebinning algorithm averages over the redundant four-dimensional set of PET data to produce a three-dimensional set of data. Images can be reconstructed from this rebinned three-dimensional set of data. This process enables one to reconstruct PET images more quickly than reconstructing directly from the four-dimensional PET data. The PFDR algorithm is an approximate rebinning algorithm. We show that implementing the PFDR algorithm followed by the (ramp) filtered backprojection (FBP) algorithm in linogram coordinates from multiple views reconstructs a filtered version of our image. We develop an explicit formula for this filter which can be used to achieve exact reconstruction by means of a modified FBP algorithm applied to the stack of rebinned linograms and can also be used to quantify the errors introduced by the PFDR algorithm. This filter is similar to the filter in the planogram filtered backprojection algorithm derived by Brasse et al. The planogram filtered backprojection and exact reconstruction with the PFDR algorithm require complete projections which can be completed with a reprojection algorithm. The PFDR algorithm is similar to the rebinning algorithm developed by Kao et al. By expressing the PFDR algorithm in detector coordinates, we provide a comparative analysis between the two algorithms. Numerical experiments using both simulated data and measured data from a positron emission mammography/tomography (PEM/PET) system are performed. Images are reconstructed by PFDR+FBP (PFDR followed by 2D FBP reconstruction), PFDRX (PFDR followed by the modified FBP algorithm for exact reconstruction) and planogram filtered backprojection image reconstruction algorithms. We show that the PFDRX algorithm produces images that are nearly as accurate as images reconstructed with the planogram filtered backprojection algorithm and more accurate than images reconstructed with the PFDR+FBP algorithm. Both the PFDR+FBP and PFDRX algorithms provide a dramatic improvement in computation time over the planogram filtered backprojection algorithm. PMID:20436790

Statistical properties of filtered pseudorandom digital sequences formed from the sum of maximum-length sequences

NASA Technical Reports Server (NTRS)

Wallace, G. R.; Weathers, G. D.; Graf, E. R.

1973-01-01

The statistics of filtered pseudorandom digital sequences called hybrid-sum sequences, formed from the modulo-two sum of several maximum-length sequences, are analyzed. The results indicate that a relation exists between the statistics of the filtered sequence and the characteristic polynomials of the component maximum length sequences. An analysis procedure is developed for identifying a large group of sequences with good statistical properties for applications requiring the generation of analog pseudorandom noise. By use of the analysis approach, the filtering process is approximated by the convolution of the sequence with a sum of unit step functions. A parameter reflecting the overall statistical properties of filtered pseudorandom sequences is derived. This parameter is called the statistical quality factor. A computer algorithm to calculate the statistical quality factor for the filtered sequences is presented, and the results for two examples of sequence combinations are included. The analysis reveals that the statistics of the signals generated with the hybrid-sum generator are potentially superior to the statistics of signals generated with maximum-length generators. Furthermore, fewer calculations are required to evaluate the statistics of a large group of hybrid-sum generators than are required to evaluate the statistics of the same size group of approximately equivalent maximum-length sequences.

Sealed vacuum canister and method for pick-up and containment of material

DOEpatents

Stoutenburgh, R.R.

1996-02-13

A vacuum canister is described including a housing with a sealed vacuum chamber having a predetermined vacuum pressure therein and a valve having a first port for fluid communication with the vacuum chamber and a second port for receiving at least one of a fluid and a particulate material. The valve is operable between a first position to seal the vacuum chamber and retain the predetermined vacuum within the vacuum chamber, and a second position to access the vacuum chamber to permit vacuum fluid flow through the valve from the second port into the vacuum chamber. The vacuum canister, in the operation to pick up material with the valve in the second position, when the second port is located adjacent at least one of a fluid and a particulate material, is effective to displace through the valve at least one of a fluid and a particulate material into the housing. The vacuum canister is desirably suitable for picking up and containing hazardous material such as radioactive material, in which the vacuum canister includes a protective layer of lead having a predetermined thickness that is effective to shield radiation emitted from the radioactive material contained within the housing. Advantageously, the vacuum canister includes a vacuum means for establishing a predetermined vacuum pressure within the vacuum chamber. 6 figs.

Sealed vacuum canister and method for pick-up and containment of material

DOEpatents

Stoutenburgh, Roger R.

1996-01-01

A vacuum canister including a housing with a sealed vacuum chamber having a predetermined vacuum pressure therein and a valve having a first port for fluid communication with the vacuum chamber and a second port for receiving at least one of a fluid and a particulate material. The valve is operable between a first position to seal the vacuum chamber and retain the predetermined vacuum within the vacuum chamber, and a second position to access the vacuum chamber to permit vacuum fluid flow through the valve from the second port into the vacuum chamber. In operation of the vacuum canister to pick up material with the valve in the second position, when the second port is located adjacent at least one of a fluid and a particulate material, is effective to displace through the valve at least one of a fluid and a particulate material into the housing. The vacuum canister is desirably suitable for picking up and containing hazardous material such as radioactive material, in which the vacuum canister includes a protective layer of lead having a predetermined thickness that is effective to shield radiation emitted from the radioactive material contained within the housing. Advantageously, the vacuum canister includes a vacuum means for establishing a predetermined vacuum pressure within the vacuum chamber.

Evaluation of deconvolution modelling applied to numerical combustion

NASA Astrophysics Data System (ADS)

Mehl, Cédric; Idier, Jérôme; Fiorina, Benoît

2018-01-01

A possible modelling approach in the large eddy simulation (LES) of reactive flows is to deconvolve resolved scalars. Indeed, by inverting the LES filter, scalars such as mass fractions are reconstructed. This information can be used to close budget terms of filtered species balance equations, such as the filtered reaction rate. Being ill-posed in the mathematical sense, the problem is very sensitive to any numerical perturbation. The objective of the present study is to assess the ability of this kind of methodology to capture the chemical structure of premixed flames. For that purpose, three deconvolution methods are tested on a one-dimensional filtered laminar premixed flame configuration: the approximate deconvolution method based on Van Cittert iterative deconvolution, a Taylor decomposition-based method, and the regularised deconvolution method based on the minimisation of a quadratic criterion. These methods are then extended to the reconstruction of subgrid scale profiles. Two methodologies are proposed: the first one relies on subgrid scale interpolation of deconvolved profiles and the second uses parametric functions to describe small scales. Conducted tests analyse the ability of the method to capture the chemical filtered flame structure and front propagation speed. Results show that the deconvolution model should include information about small scales in order to regularise the filter inversion. a priori and a posteriori tests showed that the filtered flame propagation speed and structure cannot be captured if the filter size is too large.

A study of water hardness and the prevalence of hypomagnesaemia and hypocalcaemia in healthy subjects of Surat district (Gujarat).

PubMed

Kanadhia, Kirti C; Ramavataram, Divvi Venkata Subrahmanya Shri; Nilakhe, Shreeyas Prasad Dhanpal; Patel, Swati

2014-01-01

Various sources of drinking water, with varying levels of total hardness, and calcium and magnesium concentrations, are used by populations in different regions. The use of water purifiers can compound the problem of maintaining the desired levels of hardness. An inverse relationship between various conditions, including cardiovascular disease, and hard water has been reported. Until this study, investigation of the hardness of drinking water from different sources, and serum magnesium and calcium in normal subjects from the Surat district, had not been undertaken. This study was performed to assess the concentrations of calcium and magnesium, and total hardness in filtered and non-filtered water and the relationship with serum magnesium and calcium levels in normal subjects consuming such water. Three water samples were collected, at 15-day intervals, from 12 urban and rural areas of Surat; and also 10 different brands of bottled water. Samples were analyzed for total hardness and calcium by complexometric and EDTA methods respectively. Magnesium concentrations were obtained by subtraction of the calcium concentration from total hardness. Serum samples from healthy individuals were analyzed for magnesium and calcium using calmagite and arsenazo methods respectively. The independent t-test was used to establish significance at a level of 95%. A p-value <0.05 was considered significant. Mean total hardness, and calcium and magnesium concentrations in non-filtered, rural tube-well water were much higher than in filtered water from the same area, and the magnesium concentrations were significantly higher (p = 0.038). Filtered urban municipal had lower hardness and concentrations of calcium and magnesium (p = 0.01) compared to corresponding non-filtered water. Significantly lower levels were observed in bottled water compared to rural and urban sources of water. Serum magnesium was significantly lower in the population who were consuming filtered water compared to those drinking non-filtered water (p<0.05). No such difference was observed for serum calcium. Hypomagnesemia correlates with lower magnesium concentrations in drinking water (both rural tube-well and urban municipal waters), which can be attributed to the use of water purifiers. Assuming that a person consumes two liters of drinking water per day, it is estimated that there is an average loss of 160 mg (79%) of magnesium from total waterborne magnesium levels as a result of the filtration of both rural and urban water supplies. Bottled water is too hardness as in calcium and magnesium concentrations.

Method for dissolution and stabilization of silica-rich fibers

DOEpatents

Jantzen, C.M.

1997-11-11

A method is described for dissolving silica-rich fibers such as borosilicate fibers, fiberglass and asbestos to stabilize them for disposal. The method comprises (1) immersing the fibers in hot, five-weight-percent sodium hydroxide solution until the concentration of dissolved silica reaches equilibrium and a only a residue is left (about 48 hours), then immersing the residue in hot, five-weight-percent nitric acid until the residue dissolves (about 96 hours). After adjusting the pH of the dissolved fibers to be caustic, the solution can then be added to a waste vitrification stream for safe disposal. The method is useful in disposing contaminated HEME and HEPA filters. 1 fig.

Mold Species in Dust from the International Space Station Identified and Quantified by Mold Specific Quantitative PCR

NASA Technical Reports Server (NTRS)

Vesper, Stephen J.; Wong, Wing; Kuo, C. Mike; Pierson, Duane L.

2008-01-01

Dust was collected over a period of several weeks in 2007 from various HEPA filters in the U.S. Laboratory Module of the International Space Station (ISS). The dust was returned on the Space Shuttle Atlantis, mixed, sieved, and the DNA was extracted. Using a DNA-based method called mold specific quantitative PCR (MSQPCR), 39 molds were measured in the dust. Opportunistic pathogens Aspergillus flavus and A. niger and toxin producers Penicillium chrysogenum and P. brevicompactum were found at relatively high concentrations (compared to U.S. homes). No cells of the opportunistic pathogens A. fumigatus, A. terreus, Fusarium solani or Candida albicans were detected.

Mass Spectrometric Characteristics of Prenylated Indole Derivatives from Marine-Derived Penicillium sp. NH-SL

PubMed Central

Ding, Hui; Ding, Wanjing; Ma, Zhongjun

2017-01-01

Two prenylated indole alkaloids were isolated from the ethyl acetate extracts of a marine-derived fungus Penicillium sp. NH-SL and one of them exhibited potent cytotoxic activity against mouse hepa 1c1c7 cells. In order to detect other bioactive analogs, we used liquid chromatogram tandem mass spectrometry (LC-MS/MS) to analyze the mass spectrometric characteristics of the isolated compounds as well as the crude extracts. As a result, three other analogs were detected, and their structures were deduced according to the similar fragmentation patterns. This is the first systematic report on the mass spectrometric characteristics of prenylated indole derivatives. PMID:28327529

Triple-Quantum Filtered NMR Imaging of Sodium -23 in the Human Brain

NASA Astrophysics Data System (ADS)

Keltner, John Robinson

In the past multiple-quantum filtered imaging of biexponential relaxation sodium-23 nuclei in the human brain has been limited by low signal to noise ratios; this thesis demonstrates that such imaging is feasible when using a modified gradient-selected triple-quantum filter at a repetition time which maximizes the signal to noise ratio. Nuclear magnetic resonance imaging of biexponential relaxation sodium-23 (^{23}Na) nuclei in the human brain may be useful for detecting ischemia, cancer, and pathophysiology related to manic-depression. Multiple -quantum filters may be used to selectively image biexponential relaxation ^{23}Na signals since these filters suppress single-exponential relaxation ^{23}Na signals. In this thesis, the typical repetition times (200 -300 ms) used for in vivo multiple-quantum filtered ^{23}Na experiments are shown to be approximately 5 times greater than the optimal repetition time which maximizes multiple-quantum filtered SNR. Calculations and experimental verification show that the gradient-selected triple-quantum (GS3Q) filtered SNR for ^ {23}Na in a 4% agarose gel increases by a factor of two as the repetition time decreases from 300 ms to 55 ms. It is observed that a simple reduction of repetition time also increases spurious single-quantum signals from GS3Q filtered experiments. Irreducible superoperator calculations have been used to design a modified GS3Q filter which more effectively suppresses the spurious single-quantum signals. The modified GS3Q filter includes a preparatory crusher gradient and two-step-phase cycling. Using the modified GS3Q filter and a repetition time of 70 ms, a three dimensional triple-quantum filtered image of a phantom modelling ^{23} Na in the brain was obtained. The phantom consisted of two 4 cm diameter spheres inside of a 8.5 cm x 7 cm ellipsoid. The two spheres contained 0.012 and 0.024 M ^{23}Na in 4% agarose gel. Surrounding the spheres and inside the ellipsoid was 0.03 M aqueous ^{23}Na. The image dimensions were 16 x 16 x 16 voxels with the dimension of a voxel being 1.5 x 1.5 x 1.5 cm^3. The signal to noise ratio for the GS3Q filtered ^ {23}Na signal from the 0.012 and 0.024 M ^{23}Na spheres was 17 and 30 for a 54 minute experiment at 2.35 T. (Abstract shortened by UMI.).

Vacuum force

NASA Astrophysics Data System (ADS)

Han, Yongquan

2015-03-01

To study on vacuum force, we must clear what is vacuum, vacuum is a space do not have any air and also ray. There is not exist an absolute the vacuum of space. The vacuum of space is relative, so that the vacuum force is relative. There is a certain that vacuum vacuum space exists. In fact, the vacuum space is relative, if the two spaces compared to the existence of relative vacuum, there must exist a vacuum force, and the direction of the vacuum force point to the vacuum region. Any object rotates and radiates. Rotate bend radiate- centripetal, gravity produced, relative gravity; non gravity is the vacuum force. Gravity is centripetal, is a trend that the objects who attracted wants to Centripetal, or have been do Centripetal movement. Any object moves, so gravity makes the object curve movement, that is to say, the radiation range curve movement must be in the gravitational objects, gravity must be existed in non vacuum region, and make the object who is in the region of do curve movement (for example: The earth moves around the sun), or final attracted in the form gravitational objects, and keep relatively static with attract object. (for example: objects on the earth moves but can't reach the first cosmic speed).

Subgrid-scale effects in compressible variable-density decaying turbulence

DOE PAGES

GS, Sidharth; Candler, Graham V.

2018-05-08

We present that many turbulent flows are characterized by complex scale interactions and vorticity generation caused by compressibility and variable-density effects. In the large-eddy simulation of variable-density flows, these processes manifest themselves as subgrid-scale (SGS) terms that interact with the resolved-scale flow. This paper studies the effect of the variable-density SGS terms and quantifies their relative importance. We consider the SGS terms appearing in the density-weighted Favre-filtered equations and in the unweighted Reynolds-filtered equations. The conventional form of the Reynolds-filtered momentum equation is complicated by a temporal SGS term; therefore, we derive a new form of the Reynolds-filtered governing equationsmore » that does not contain this term and has only double-correlation SGS terms. The new form of the filtered equations has terms that represent the SGS mass flux, pressure-gradient acceleration and velocity-dilatation correlation. To evaluate the dynamical significance of the variable-density SGS effects, we carry out direct numerical simulations of compressible decaying turbulence at a turbulent Mach number of 0.3. Two different initial thermodynamic conditions are investigated: homentropic and a thermally inhomogeneous gas with regions of differing densities. The simulated flow fields are explicitly filtered to evaluate the SGS terms. The importance of the variable-density SGS terms is quantified relative to the SGS specific stress, which is the only SGS term active in incompressible constant-density turbulence. It is found that while the variable-density SGS terms in the homentropic case are negligible, they are dynamically significant in the thermally inhomogeneous flows. Investigation of the variable-density SGS terms is therefore important, not only to develop variable-density closures but also to improve the understanding of scale interactions in variable-density flows.« less

Improved Image Quality in Head and Neck CT Using a 3D Iterative Approach to Reduce Metal Artifact.

PubMed

Wuest, W; May, M S; Brand, M; Bayerl, N; Krauss, A; Uder, M; Lell, M

2015-10-01

Metal artifacts from dental fillings and other devices degrade image quality and may compromise the detection and evaluation of lesions in the oral cavity and oropharynx by CT. The aim of this study was to evaluate the effect of iterative metal artifact reduction on CT of the oral cavity and oropharynx. Data from 50 consecutive patients with metal artifacts from dental hardware were reconstructed with standard filtered back-projection, linear interpolation metal artifact reduction (LIMAR), and iterative metal artifact reduction. The image quality of sections that contained metal was analyzed for the severity of artifacts and diagnostic value. A total of 455 sections (mean ± standard deviation, 9.1 ± 4.1 sections per patient) contained metal and were evaluated with each reconstruction method. Sections without metal were not affected by the algorithms and demonstrated image quality identical to each other. Of these sections, 38% were considered nondiagnostic with filtered back-projection, 31% with LIMAR, and only 7% with iterative metal artifact reduction. Thirty-three percent of the sections had poor image quality with filtered back-projection, 46% with LIMAR, and 10% with iterative metal artifact reduction. Thirteen percent of the sections with filtered back-projection, 17% with LIMAR, and 22% with iterative metal artifact reduction were of moderate image quality, 16% of the sections with filtered back-projection, 5% with LIMAR, and 30% with iterative metal artifact reduction were of good image quality, and 1% of the sections with LIMAR and 31% with iterative metal artifact reduction were of excellent image quality. Iterative metal artifact reduction yields the highest image quality in comparison with filtered back-projection and linear interpolation metal artifact reduction in patients with metal hardware in the head and neck area. © 2015 by American Journal of Neuroradiology.

Studies on the muscle-paralyzing components of the juice of the banana plant.

PubMed

Singh, Y N; Inman, W D; Johnson, A; Linnell, E J

1993-01-01

The stem juice of the banana plant (Musa species) has been used as an arrow poison by African tribesmen. Lyophilized, partially purified extracts of the juice augment and then block both directly and indirectly evoked contractions of the mouse diaphragm. We have isolated, purified and determined the chemical composition of the active ingredients, and characterized their pharmacological activity. The lyophilized sample was extracted with a methanol-water (MeOH-H2O) (50/50) mixture and vacuum filtered. The filtrate was rotary evaporated and crystallized in a MeOH-H2O mixture to yield potassium nitrate crystals (melting point 332-334 degrees C). The filtrate was concentrated and chromatographed over Sephadex LH-20 gel using MeOH-H2O (40/60) as the eluent. The active component was found to be magnesium nitrate crystals (melting point 87-89 degrees C). In the mouse isolated phrenic nerve-hemidiaphragm preparation, the pharmacological profile of the first component was similar to that for authentic potassium nitrate which augments in low concentrations, and in higher concentrations augments, and then blocks both directly evoked muscle contraction the neuromuscular transmission. The second component had a profile of activity similar to that for authentic magnesium nitrate which only blocks neuromuscular transmission. It can be concluded that the two major active principles in the banana stem juice are potassium nitrate and magnesium nitrate.

Two techniques enable sampling of filtered and unfiltered molten metals

NASA Technical Reports Server (NTRS)

Burris, L., Jr.; Pierce, R. D.; Tobias, K. R.; Winsch, I. O.

1967-01-01

Filtered samples of molten metals are obtained by filtering through a plug of porous material fitted in the end of a sample tube, and unfiltered samples are obtained by using a capillary-tube extension rod with a perforated bucket. With these methods there are no sampling errors or loss of liquid.

Mercury photoreduction and photooxidation in lakes: Effects of filtration and dissolved organic carbon concentration.

PubMed

O'Driscoll, Nelson J; Vost, Emma; Mann, Erin; Klapstein, Sara; Tordon, Robert; Lukeman, Matthew

2018-06-01

Mercury is a globally distributed, environmental contaminant. Quantifying the retention and loss of mercury is integral for predicting mercury-sensitive ecosystems. There is little information on how dissolved organic carbon (DOC) concentrations and particulates affect mercury photoreaction kinetics in freshwater lakes. To address this knowledge gap, samples were collected from ten lakes in Kejimkujik National Park, Nova Scotia (DOC: 2.6-15.4mg/L). Filtered (0.2μm) and unfiltered samples were analysed for gross photoreduction, gross photooxidation, and net reduction rates of mercury using pseudo first-order curves. Unfiltered samples had higher concentrations (p=0.04) of photoreducible divalent mercury (Hg(II) RED ) (mean of 754±253pg/L) than filtered samples (mean of 482±206pg/L); however, gross photoreduction and photooxidation rate constants were not significantly different in filtered or unfiltered samples in early summer. DOC was not significantly related to gross photoreduction rate constants in filtered (R 2 =0.43; p=0.08) and unfiltered (R 2 =0.02; p=0.71) samples; DOC was also not significantly related to gross photooxidation rate constants in filtered or unfiltered samples. However, DOC was significantly negatively related with Hg(II) RED in unfiltered (R 2 =0.53; p=0.04), but not in filtered samples (R 2 =0.04; p=0.60). These trends indicate that DOC is a factor in determining dissolved mercury photoreduction rates and particles partially control available Hg(II) RED in lake water. This research also demonstrates that within these lakes gross photoreduction and photooxidation processes are close to being in balance. Changes to catchment inputs of particulate matter and DOC may alter mercury retention in these lakes and could partially explain observed increases of mercury accumulation in biota. Copyright © 2017. Published by Elsevier B.V.

Detection of Non-Photochemical Superoxide in Coastal and Open Ocean Seawater: Particulate Versus Dissolved Sources

NASA Astrophysics Data System (ADS)

Roe, K. L.; Rand, T.; Hansel, C. M.; Voelker, B. M.

2016-02-01

Superoxide radical (O2-) could have a significant effect on marine metal redox chemistry, but little data exists on its marine concentrations. In this study, we measured superoxide steady-state concentrations in both filtered and unfiltered samples collected near the California coast and at Station ALOHA. Particle-generated superoxide, defined as the difference between unfiltered and filtered concentrations, ranged from undetectable to 0.019 nM at Station ALOHA and from undetectable to 0.052 nM in samples from the southern California Current. We also show that a transient superoxide signal is generated during filtering, an artifact that may have affected previously reported concentrations of particle-generated superoxide in the ocean. High concentrations of superoxide (range) were measured in filtered samples from ALOHA station and the California Current, raising concerns about possible sources of background signals. Further study of background signals revealed that some superoxide production occurs even in artificial seawater and very aged filtered seawater samples, and that a small additional background signal is generated as the sample travels from the container to the flow cell where it is mixed with reagent for CL analysis. However, filtered seawater samples collected from the Scripps Pier had significantly higher superoxide production rates than those measured in artificial seawater, and production rates in unfiltered samples were no higher than those in filtered samples. Therefore, production by dissolved sources was the dominant non-photochemical source of superoxide in these samples. Production rates decreased in the presence of DTPA, suggesting involvement of metal ions in superoxide production. Laboratory experiments with natural organic matter (NOM) indicate that superoxide formation occurs during oxidation of reduced moieties of NOM by oxygen.

A Randomized Trial of Phototherapy with Filtered Sunlight in African Neonates.

PubMed

Slusher, Tina M; Olusanya, Bolajoko O; Vreman, Hendrik J; Brearley, Ann M; Vaucher, Yvonne E; Lund, Troy C; Wong, Ronald J; Emokpae, Abieyuwa A; Stevenson, David K

2015-09-17

Sequelae of severe neonatal hyperbilirubinemia constitute a substantial disease burden in areas where effective conventional phototherapy is unavailable. We previously found that the use of filtered sunlight for the purpose of phototherapy is a safe and efficacious method for reducing total bilirubin. However, its relative safety and efficacy as compared with conventional phototherapy are unknown. We conducted a randomized, controlled noninferiority trial in which filtered sunlight was compared with conventional phototherapy for the treatment of hyperbilirubinemia in term and late-preterm neonates in a large, urban Nigerian maternity hospital. The primary end point was efficacy, which was defined as a rate of increase in total serum bilirubin of less than 0.2 mg per deciliter per hour for infants up to 72 hours of age or a decrease in total serum bilirubin for infants older than 72 hours of age who received at least 5 hours of phototherapy; we prespecified a noninferiority margin of 10% for the difference in efficacy rates between groups. The need for an exchange transfusion was a secondary end point. We also assessed safety, which was defined as the absence of the need to withdraw therapy because of hyperthermia, hypothermia, dehydration, or sunburn. We enrolled 447 infants and randomly assigned 224 to filtered sunlight and 223 to conventional phototherapy. Filtered sunlight was efficacious on 93% of treatment days that could be evaluated, as compared with 90% for conventional phototherapy, and had a higher mean level of irradiance (40 vs. 17 μW per square centimeter per nanometer, P<0.001). Temperatures higher than 38.0°C occurred in 5% of the infants receiving filtered sunlight and in 1% of those receiving conventional phototherapy (P<0.001), but no infant met the criteria for withdrawal from the study for reasons of safety or required an exchange transfusion. Filtered sunlight was noninferior to conventional phototherapy for the treatment of neonatal hyperbilirubinemia and did not result in any study withdrawals for reasons of safety. (Funded by the Thrasher Research Fund, Salt Lake City, and the National Center for Advancing Translational Sciences of the National Institutes of Health; Clinical Trials.gov number, NCT01434810.).

Filtered Life Satisfaction and Its Socioeconomic Determinants in Hong Kong

ERIC Educational Resources Information Center

Cheung, Chau-kiu; Ngan, Raymond Man-hung

2012-01-01

Filtering the measure of life satisfaction through the bias of social desirability and response styles would furnish an adequate analysis of socioeconomic impacts on the filtered life satisfaction. The filtering is necessary because social desirability and the response styles of acquiescence, extremity, and centrality are likely to contaminate the…

Evidence of Immunosuppressive and Th2 Immune Polarizing Effects of Antidiabetic Momordica charantia Fruit Juice

PubMed Central

Amoussa, Abdou Madjid; Adjagba, Marius; Lagnika, Latifou; Lalèyè, Anatole

2017-01-01

The mechanism of action of the antidiabetic capacity of Momordica charantia is still under investigation. Here, we assessed phytochemical compositions, antioxidant activity, and effects of total and filtered fruit and leafy stem juices of Momordica charantia on human T cell proliferation and differentiation through quantification of Th1/Th2 cytokines. In the absence of stimulation, total fruit and leafy stem juices induced significant T cell proliferation. Under PHA stimulation, both juices potentiated plant-induced T cell proliferation. However, the filtered fruit and leafy stem juices significantly inhibited PHA-stimulated T cell proliferation, while neither juice influenced T cell proliferation. Moreover, total and filtered fruit juice increased IL-4 secretion, while total and filtered leafy stem juice enhanced IFN-γ production. Phytochemical screening revealed the presence of tannins, flavonoids, anthocyans, steroids, and triterpenoids in both juices. Alkaloids, quinone derivatives, cardenolides, and cyanogenic derivatives were undetectable. The saponins present in total juices were undetectable after filtration. Moreover, both juices had appreciable antioxidant capacity. Our study supports the type 1 antidiabetic effect of filtered fruit juice of M. charantia which may be related to its immunosuppressive and T-helper 2 cell inducing capacities. Due to their immune-stimulatory activities and their ability to increase T-helper 1 cell cytokines, total fruit and leafy stem juices may serve in the treatment of immunodeficiency and certain infections. PMID:28812026

Subgrid-scale effects in compressible variable-density decaying turbulence

DOE Office of Scientific and Technical Information (OSTI.GOV)

GS, Sidharth; Candler, Graham V.

We present that many turbulent flows are characterized by complex scale interactions and vorticity generation caused by compressibility and variable-density effects. In the large-eddy simulation of variable-density flows, these processes manifest themselves as subgrid-scale (SGS) terms that interact with the resolved-scale flow. This paper studies the effect of the variable-density SGS terms and quantifies their relative importance. We consider the SGS terms appearing in the density-weighted Favre-filtered equations and in the unweighted Reynolds-filtered equations. The conventional form of the Reynolds-filtered momentum equation is complicated by a temporal SGS term; therefore, we derive a new form of the Reynolds-filtered governing equationsmore » that does not contain this term and has only double-correlation SGS terms. The new form of the filtered equations has terms that represent the SGS mass flux, pressure-gradient acceleration and velocity-dilatation correlation. To evaluate the dynamical significance of the variable-density SGS effects, we carry out direct numerical simulations of compressible decaying turbulence at a turbulent Mach number of 0.3. Two different initial thermodynamic conditions are investigated: homentropic and a thermally inhomogeneous gas with regions of differing densities. The simulated flow fields are explicitly filtered to evaluate the SGS terms. The importance of the variable-density SGS terms is quantified relative to the SGS specific stress, which is the only SGS term active in incompressible constant-density turbulence. It is found that while the variable-density SGS terms in the homentropic case are negligible, they are dynamically significant in the thermally inhomogeneous flows. Investigation of the variable-density SGS terms is therefore important, not only to develop variable-density closures but also to improve the understanding of scale interactions in variable-density flows.« less

Design Characteristics and Tobacco Metal Concentrations in Filtered Cigars.

PubMed

Caruso, Rosalie V; O'Connor, Richard J; Travers, Mark J; Delnevo, Cristine D; Stephens, W Edryd

2015-11-01

While U.S. cigarette consumption has declined, cigar use has steadily increased, for reasons including price compared to cigarettes and the availability of filtered varieties resembling cigarettes, and flavors that have been banned in cigarettes (excluding menthol). Little published data exists on the design characteristics of such cigars. A variety of filtered cigar brands were tested for design characteristics such as whole cigar weight, ventilation, and per-cigar tobacco weight. Cigar sticks were then sent to the University of St. Andrews for metal concentration testing of As, Pb, Cr, Ni, and Cd. Large and small cigars were statistically different between cigar weight (p ≤ .001), per-cigar tobacco weight (p = .001), rod diameter (p = .006), and filter diameter (p = .012). The differences in mean ventilation (overall mean = 19.6%, min. = 0.84%, max. = 57.6%) across filtered cigar brands were found to be statistically significant (p = .031), and can be compared to the ventilation of the average of 2013 U.S. Marlboro Red, Gold, and Silver packs at 29% ventilation. There were no significant differences for metal concentrations between cigar types (p = .650), with Pb and As levels being similar to U.S. 2009 cigarette concentrations, Cd cigar levels being slightly higher, and Cr and Ni levels much lower than cigarette levels. With cigar use rising, and filtered cigars displaying substantial similarities to filtered cigarettes, more research on product characteristics is warranted. Future plans include testing tobacco alkaloid and more observation of cigar weight for tax bracket purposes. © The Author 2015. Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Ion implanted dielectric elastomer circuits

NASA Astrophysics Data System (ADS)

O'Brien, Benjamin M.; Rosset, Samuel; Anderson, Iain A.; Shea, Herbert R.

2013-06-01

Starfish and octopuses control their infinite degree-of-freedom arms with panache—capabilities typical of nature where the distribution of reflex-like intelligence throughout soft muscular networks greatly outperforms anything hard, heavy, and man-made. Dielectric elastomer actuators show great promise for soft artificial muscle networks. One way to make them smart is with piezo-resistive Dielectric Elastomer Switches (DES) that can be combined with artificial muscles to create arbitrary digital logic circuits. Unfortunately there are currently no reliable materials or fabrication process. Thus devices typically fail within a few thousand cycles. As a first step in the search for better materials we present a preliminary exploration of piezo-resistors made with filtered cathodic vacuum arc metal ion implantation. DES were formed on polydimethylsiloxane silicone membranes out of ion implanted gold nano-clusters. We propose that there are four distinct regimes (high dose, above percolation, on percolation, low dose) in which gold ion implanted piezo-resistors can operate and present experimental results on implanted piezo-resistors switching high voltages as well as a simple artificial muscle inverter. While gold ion implanted DES are limited by high hysteresis and low sensitivity, they already show promise for a range of applications including hysteretic oscillators and soft generators. With improvements to implanter process control the promise of artificial muscle circuitry for soft smart actuator networks could become a reality.

A FED Prototype Using Patterned DLC Thin Films as the Cathode

NASA Astrophysics Data System (ADS)

Li, W.; Feng, T.; Mao, D. S.; Wang, X.; Liu, X. H.; Zou, S. C.; Zhu, Y. K.; Li, Q.; Xu, J. F.; Jin, S.; Zheng, J. S.

In our study, diamond-like-carbon (DLC) thin films were prepared by filtered arc deposition (FAD), which provided a way to deposit DLC thin films on large areas at room temperature. Glass slides coated 100nm chromium or titanium thin films were used as cathode substrates. Millions of rectangular holes with sizes of 5 × 5μm were made on the DLC films using a routine patterning process. Here a special reactive ion beam etching method was applied to etch the DLC films. The anodes of the devices were made by electrophoretic deposition. ZnO:Zn phosphor (P15) was employed, which has a broad band bluish green (centered at 490nm). Before electrophoretic deposition, the anode substrates (ITO glass slides) had been patterned into 50 anode electrodes. In order to improve the adherence of phosphor layers, the as-deposited screens were treated in Na2SiO3 solution for 24h to add additional binder. A kind of matrix-addressed diode FED prototype was designed and packaged. 50-100μm-thick glass slides were used as spacers and getters were applied to maintain the vacuum after the exhaustion. The applied DC voltage was ranged in 0-3000V and much higher current density was measured in the cathode-patterned prototypes than the unpatterned ones during the test. As a result, characters could be well displayed.

A Priori Subgrid Scale Modeling for a Droplet Laden Temporal Mixing Layer

NASA Technical Reports Server (NTRS)

Okongo, Nora; Bellan, Josette

2000-01-01

Subgrid analysis of a transitional temporal mixing layer with evaporating droplets has been performed using a direct numerical simulation (DNS) database. The DNS is for a Reynolds number (based on initial vorticity thickness) of 600, with droplet mass loading of 0.2. The gas phase is computed using a Eulerian formulation, with Lagrangian droplet tracking. Since Large Eddy Simulation (LES) of this flow requires the computation of unfiltered gas-phase variables at droplet locations from filtered gas-phase variables at the grid points, it is proposed to model these by assuming the gas-phase variables to be given by the filtered variables plus a correction based on the filtered standard deviation, which can be computed from the sub-grid scale (SGS) standard deviation. This model predicts unfiltered variables at droplet locations better than simply interpolating the filtered variables. Three methods are investigated for modeling the SGS standard deviation: Smagorinsky, gradient and scale-similarity. When properly calibrated, the gradient and scale-similarity methods give results in excellent agreement with the DNS.

Direct flow crystal growth system

DOEpatents

Montgomery, Kenneth E.; Milanovich, Fred P.

1992-01-01

A crystal is grown in a constantly filtered solution which is flowed directly into the growing face of a crystal. In a continuous flow system, solution at its saturation temperature is removed from a crystal growth tank, heated above its saturation temperature, filtered, cooled back to its saturation temperature, and returned to the tank.

Filtered Density Function for Subgrid Scale Modeling of Turbulent Combustion

DTIC Science & Technology

2009-02-25

and Plasma Chemistry (Russian), 6(1), 1-6 (2008). M.R.H. Sheikhi, P. Givi and S.B. Pope, "Joint Velocity-Scalar Filtered Mass Density Function for...4th International Symposium on Combustion and Plasma Chemistry , pp. 18-20, Almaty, Kazakhstan, September 12-14, 2007. M.R.H. Sheikhi, P. Givi and

Tailoring noise frequency spectrum between two consecutive second derivative filtering procedures to improve liquid chromatography-mass spectrometry determinations.

PubMed

Wang, Shau-Chun; Lin, Chiao-Juan; Chiang, Shu-Min; Yu, Sung-Nien

2008-03-15

This paper reports a simple chemometric technique to alter the noise spectrum of a liquid chromatography-mass spectrometry (LC-MS) chromatogram between two consecutive second-derivative filter procedures to improve the peak signal-to-noise (S/N) ratio enhancement. This technique is to multiply one second-derivative filtered LC-MS chromatogram with another artificial chromatogram added with thermal noises prior to the other second-derivative filter. Because the second-derivative filter cannot eliminate frequency components within its own filter bandwidth, more efficient peak S/N ratio improvement cannot be accomplished using consecutive second-derivative filter procedures to process LC-MS chromatograms. In contrast, when the second-derivative filtered LC-MS chromatogram is conditioned with the multiplication alteration prior to the other second-derivative filter, much better ratio improvement is achieved. The noise frequency spectrum of the second-derivative filtered chromatogram, which originally contains frequency components within the filter bandwidth, is altered to span a broader range with multiplication operation. When the frequency range of this modified noise spectrum shifts toward the other regimes, the other second-derivative filter, working as a band-pass filter, is able to provide better filtering efficiency to obtain higher peak S/N ratios. Real LC-MS chromatograms, of which 5-fold peak S/N ratio improvement achieved with two consecutive second-derivative filters remains the same S/N ratio improvement using a one-step second-derivative filter, are improved to accomplish much better ratio enhancement, approximately 25-fold or higher when the noise frequency spectrum is modified between two matched filters. The linear standard curve using the filtered LC-MS signals is validated. The filtered LC-MS signals are also more reproducible. The more accurate determinations of very low-concentration samples (S/N ratio about 5-7) are obtained via standard addition procedures using the filtered signals rather than the determinations using the original signals.

Friction Durability of Extremely Thin Diamond-Like Carbon Films at High Temperature

PubMed Central

Miyake, Shojiro; Suzuki, Shota; Miyake, Masatoshi

2017-01-01

To clarify the friction durability, both during and after the high-temperature heating of nanometer-thick diamond-like carbon (DLC) films, deposited using filtered cathodic vacuum arc (FCVA) and plasma chemical vapor deposition (P-CVD) methods, the dependence of the friction coefficient on the load and sliding cycles of the DLC films, were evaluated. Cluster-I consisted of a low friction area in which the DLC film was effective, while cluster-II consisted of a high friction area in which the lubricating effect of the DLC film was lost. The friction durability of the films was evaluated by statistical cluster analysis. Extremely thin FCVA-DLC films exhibited an excellent wear resistance at room temperature, but their friction durability was decreased at high temperatures. In contrast, the durability of the P-CVD-DLC films was increased at high temperatures when compared with that observed at room temperature. This inverse dependence on temperature corresponded to the nano-friction results obtained by atomic force microscopy. The decrease in the friction durability of the FCVA-DLC films at high temperatures, was caused by a complex effect of temperature and friction. The tribochemical reaction produced by the P-CVD-DLC films reduced their friction coefficient, increasing their durability at high temperatures. PMID:28772520

Multilayer composition coatings for cutting tools: formation and performance properties

NASA Astrophysics Data System (ADS)

Tabakov, Vladimir P.; Vereschaka, Anatoly S.; Vereschaka, Alexey A.

2018-03-01

The paper considers the concept of a multi-layer architecture of the coating in which each layer has a predetermined functionality. Latest generation of coatings with multi-layered architecture for cutting tools secure a dual nature of the coating, in which coatings should not only improve the mechanical and physical characteristics of the cutting tool material, but also reduce the thermo-mechanical effect on the cutting tool determining wear intensity. Here are presented the results of the development of combined methods of forming multi-layer coatings with improved properties. Combined method of forming coatings using a pulsed laser allowed reducing excessively high levels of compressive residual stress and increasing micro hardness of the multilayered coatings. The results in testing coated HSS tools showed that the use of additional pulse of laser processing increases tool life up to 3 times. Using filtered cathodic vacuum arc deposition for the generation of multilayer coatings based on TiAlN compound has increased the wear-resistance of carbide tools by 2 fold compared with tool life of cutting tool with commercial TiN coatings. The aim of this study was to develop an innovative methodological approach to the deposition of multilayer coatings for cutting tools with functional architectural selection, properties and parameters of the coating based on sound knowledge of coating failure in machining process.

Friction Durability of Extremely Thin Diamond-Like Carbon Films at High Temperature.

PubMed

Miyake, Shojiro; Suzuki, Shota; Miyake, Masatoshi

2017-02-10

To clarify the friction durability, both during and after the high-temperature heating of nanometer-thick diamond-like carbon (DLC) films, deposited using filtered cathodic vacuum arc (FCVA) and plasma chemical vapor deposition (P-CVD) methods, the dependence of the friction coefficient on the load and sliding cycles of the DLC films, were evaluated. Cluster-I consisted of a low friction area in which the DLC film was effective, while cluster-II consisted of a high friction area in which the lubricating effect of the DLC film was lost. The friction durability of the films was evaluated by statistical cluster analysis. Extremely thin FCVA-DLC films exhibited an excellent wear resistance at room temperature, but their friction durability was decreased at high temperatures. In contrast, the durability of the P-CVD-DLC films was increased at high temperatures when compared with that observed at room temperature. This inverse dependence on temperature corresponded to the nano-friction results obtained by atomic force microscopy. The decrease in the friction durability of the FCVA-DLC films at high temperatures, was caused by a complex effect of temperature and friction. The tribochemical reaction produced by the P-CVD-DLC films reduced their friction coefficient, increasing their durability at high temperatures.

OFDM-Based Signal Explotation Using Quadrature Mirror Filter Bank (QMFB) Processing

DTIC Science & Technology

2012-03-01

need in childhood as strong as the need for a father’s protection. Sigmund Freud vi Acknowledgments First I would like to thank to my...Real part of the filtered signal Q = imag(I); % Imaginary part of the filtered signal % Saving the data to the same directory save

Overview of Fabrication Techniques and Lessons Learned with Accelerator Vacuum Windows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ader, C. R.; McGee, M. W.; Nobrega, L. E.

Vacuum thin windows have been used in Fermilab's accelerators for decades and typically have been overlooked in terms of their criticality and fragility. Vacuum windows allow beam to pass through while creating a boundary between vacuum and air or high vacuum and low vacuum areas. The design of vacuum windows, including Titanium and Beryllium windows, will be discussed as well as fabrication, testing, and operational concerns. Failure of windows will be reviewed as well as safety approaches to mitigating failures and extending the lifetimes of vacuum windows. Various methods of calculating the strengths of vacuum windows will be explored, includingmore » FEA.« less

49 CFR 572.189 - Instrumentation and test conditions.

Code of Federal Regulations, 2013 CFR

2013-10-01

.... The sum mass of the attachments and 1/3 cable mass must not exceed 5 percent of the total pendulum... filtered CFC 180; (3)Neck and lumbar spine pendulum accelerations—Digitally filtered CFC 60; (4) Pelvis... 180. (j)(1) Filter the pendulum acceleration data using a SAE J211 CFC 60 filter. (2) Determine the...

Individual snag detection using neighborhood attribute filtered airborne lidar data

Treesearch

Brian M. Wing; Martin W. Ritchie; Kevin Boston; Warren B. Cohen; Michael J. Olsen

2015-01-01

The ability to estimate and monitor standing dead trees (snags) has been difficult due to their irregular and sparse distribution, often requiring intensive sampling methods to obtain statistically significant estimates. This study presents a new method for estimating and monitoring snags using neighborhood attribute filtered airborne discrete-return lidar data. The...

Properties of the Residual Stress of the Temporally Filtered Navier-Stokes Equations

NASA Technical Reports Server (NTRS)

Pruett, C. D.; Gatski, T. B.; Grosch, C. E.; Thacker, W. D.

2002-01-01

The development of a unifying framework among direct numerical simulations, large-eddy simulations, and statistically averaged formulations of the Navier-Stokes equations, is of current interest. Toward that goal, the properties of the residual (subgrid-scale) stress of the temporally filtered Navier-Stokes equations are carefully examined. Causal time-domain filters, parameterized by a temporal filter width 0 less than Delta less than infinity, are considered. For several reasons, the differential forms of such filters are preferred to their corresponding integral forms; among these, storage requirements for differential forms are typically much less than for integral forms and, for some filters, are independent of Delta. The behavior of the residual stress in the limits of both vanishing and in infinite filter widths is examined. It is shown analytically that, in the limit Delta to 0, the residual stress vanishes, in which case the Navier-Stokes equations are recovered from the temporally filtered equations. Alternately, in the limit Delta to infinity, the residual stress is equivalent to the long-time averaged stress, and the Reynolds-averaged Navier-Stokes equations are recovered from the temporally filtered equations. The predicted behavior at the asymptotic limits of filter width is further validated by numerical simulations of the temporally filtered forced, viscous Burger's equation. Finally, finite filter widths are also considered, and a priori analyses of temporal similarity and temporal approximate deconvolution models of the residual stress are conducted.

Evidence of Immunosuppressive and Th2 Immune Polarizing Effects of Antidiabetic Momordica charantia Fruit Juice.

PubMed

Fachinan, Rufine; Fagninou, Adnette; Nekoua, Magloire Pandoua; Amoussa, Abdou Madjid; Adjagba, Marius; Lagnika, Latifou; Lalèyè, Anatole; Moutairou, Kabirou; Yessoufou, Akadiri

2017-01-01

The mechanism of action of the antidiabetic capacity of Momordica charantia is still under investigation. Here, we assessed phytochemical compositions, antioxidant activity, and effects of total and filtered fruit and leafy stem juices of Momordica charantia on human T cell proliferation and differentiation through quantification of Th1/Th2 cytokines. In the absence of stimulation, total fruit and leafy stem juices induced significant T cell proliferation. Under PHA stimulation, both juices potentiated plant-induced T cell proliferation. However, the filtered fruit and leafy stem juices significantly inhibited PHA-stimulated T cell proliferation, while neither juice influenced T cell proliferation. Moreover, total and filtered fruit juice increased IL-4 secretion, while total and filtered leafy stem juice enhanced IFN- γ production. Phytochemical screening revealed the presence of tannins, flavonoids, anthocyans, steroids, and triterpenoids in both juices. Alkaloids, quinone derivatives, cardenolides, and cyanogenic derivatives were undetectable. The saponins present in total juices were undetectable after filtration. Moreover, both juices had appreciable antioxidant capacity. Our study supports the type 1 antidiabetic effect of filtered fruit juice of M. charantia which may be related to its immunosuppressive and T-helper 2 cell inducing capacities. Due to their immune-stimulatory activities and their ability to increase T-helper 1 cell cytokines, total fruit and leafy stem juices may serve in the treatment of immunodeficiency and certain infections.

Virucidal effects of rodent cage-cleaning practices on the viability of adenovirus vectors.

PubMed

Porter, Jacqueline D; Lyons, Russette M

2002-09-01

Human adenoviruses and adenoviral vectors are classified as Risk Group 2 agents and require BSL2 containment and practices. An additional consideration in using adenoviruses and viral vectors in laboratory animal studies is the possible transmission of these agents to other animals and/or personnel as a result of viral shedding in animal urine and feces. When handling BSL2 agents, cage-wash staff are required to wear appropriate personnel protective equipment, including scrubs, Tyvek suit, hair covering, dust mask, shoes covers, and gloves. Current decontamination procedures are to bag and autoclave soiled rodent cages containing bedding prior to washing in the cage washer to prevent possible adenoviral transmission. However, the practice of autoclaving softens the polycarbonate-based rodent cages, allowing damaging agents or conditions to affect the integrity of the plastic and degrade the cages. The objective of this study was to determine whether current rodent cage-cleaning practices produced virucidal effects for use in lieu of or prior to autoclaving the cages. We found that heating an Av3GFP vector in a test tube to a temperature of 74 degrees C (165 degrees F) for 6 min conditions equivalent to those of the cage washer resulted in greater than an 11-log reduction in infectivity of the vector as evaluated by its cytopathic effect on cells. The combination of heating and a liquid, phosphate-free alkaline detergent produced the same reduction in vector infectivity. However, common cage-cleaning solutions alone possessed no virucidal activity. The high temperatures used in cage-washing procedures alone or in combination with a cleaning solution reduced or eliminated the risk of transmission from viral shedding through urine and feces even at vector concentrations far greater than would ever be expected to be present. Autoclaving cages diminishes the stability and integrity of the polycarbonate cages without providing a further reduction in the risk of virus or vector transmission. On the basis of results from this study, new cage-wash recommendations include dumping the contaminated bedding into a HEPA-filtered waste disposal system and autoclaving the bags of bedding before disposal, then cleaning the cages in the rack washer at wash temperatures of 74 degrees C (165 F) and rinse temperatures of 82 degrees C (180 F).

49 CFR 570.56 - Vacuum brake assist unit and vacuum brake system.

Code of Federal Regulations, 2010 CFR

2010-10-01

.... The following requirements apply to vehicles with vacuum brake assist units and vacuum brake systems. (a) Vacuum brake assist unit integrity. The vacuum brake assist unit shall demonstrate integrity as... maintained on the pedal. (1) Inspection procedure. Stop the engine and apply service brake several times to...

Covert Half Duplex Data Link Using Radar-Embedded Communications With Various Modulation Schemes

DTIC Science & Technology

2017-12-01

27 Figure 4.1 Comparison of Theoretical, Radar-Pulse-Only Matched Filter , and the Radar...CommunicationsMatched Filtered PD Curves versus SNR for RCR = 0 dB. . . . . . . . . . . . . . . . . . . . . . . . . . . 30 Figure 4.2 Comparison of Theoretical, Radar...Pulse-Only Matched Filter , and the Radar-CommunicationsMatched Filtered PD Curves versus SNR for RCR = [3, 6, 10] dB

Influence of Gap Distance on Vacuum Arc Characteristics of Cup Type AMF Electrode in Vacuum Interrupters

NASA Astrophysics Data System (ADS)

Cheng, Shaoyong; Xiu, Shixin; Wang, Jimei; Shen, Zhengchao

2006-11-01

The greenhouse effect of SF6 is a great concern today. The development of high voltage vacuum circuit breakers becomes more important. The vacuum circuit breaker has minimum pollution to the environment. The vacuum interrupter is the key part of a vacuum circuit breaker. The interrupting characteristics in vacuum and arc-controlling technique are the main problems to be solved for a longer gap distance in developing high voltage vacuum interrupters. To understand the vacuum arc characteristics and provide effective technique to control vacuum arc in a long gap distance, the arc mode transition of a cup-type axial magnetic field electrode is observed by a high-speed charge coupled device (CCD) video camera under different gap distances while the arc voltage and arc current are recorded. The controlling ability of the axial magnetic field on vacuum arc obviously decreases when the gap distance is longer than 40 mm. The noise components and mean value of the arc voltage significantly increase. The effective method for controlling the vacuum arc characteristics is provided by long gap distances based on the test results. The test results can be used as a reference to develop high voltage and large capacity vacuum interrupters.

Simulation study of accelerator based quasi-mono-energetic epithermal neutron beams for BNCT.

PubMed

Adib, M; Habib, N; Bashter, I I; El-Mesiry, M S; Mansy, M S

2016-01-01

Filtered neutron techniques were applied to produce quasi-mono-energetic neutron beams in the energy range of 1.5-7.5 keV at the accelerator port using the generated neutron spectrum from a Li (p, n) Be reaction. A simulation study was performed to characterize the filter components and transmitted beam lines. The feature of the filtered beams is detailed in terms of optimal thickness of the primary and additive components. A computer code named "QMNB-AS" was developed to carry out the required calculations. The filtered neutron beams had high purity and intensity with low contamination from the accompanying thermal, fast neutrons and γ-rays. Copyright © 2015 Elsevier Ltd. All rights reserved.

Does interspecific competition alter effects of early season ozone exposure on plants from wet grasslands? Results of a three-year experiment in open-top chambers.

PubMed

Tonneijck, A E G; Franzaring, J; Brouwer, G; Metselaar, K; Dueck, Th A

2004-09-01

Chronic effects of ozone on wet grassland species early in the growing season might be altered by interspecific competition. Individual plants of Holcus lanatus, Lychnis flos-cuculi, Molinia caerulea and Plantago lanceolata were grown in monocultures and in mixed cultures with Agrostis capillaris. Mesocosms were exposed to charcoal-filtered air plus 25 nl l(-1) ozone (CF+25), non-filtered air (NF), non-filtered air plus 25 nl l(-1) ozone (NF+25) and non-filtered air plus 50 nl l(-1) ozone (NF+50) early in the growing seasons of 2000 through 2002. Ozone-enhanced senescence and visible foliar injury were recorded on some of the target plants in the first year only. Ozone effects on biomass production were minimal and plant response to ozone did not differ between monocultures and mixed cultures. After three years, above-ground biomass of the plants in mixed culture compared to monocultures was three times greater for H. lanatus and two to four times smaller for the other species.

Comparison of vacuum and non-vacuum urine tubes for urinary sediment analysis.

PubMed

Topcuoglu, Canan; Sezer, Sevilay; Kosem, Arzu; Ercan, Mujgan; Turhan, Turan

2017-12-01

Urine collection systems with aspiration system for vacuum tubes are becoming increasingly common for urinalysis, especially for microscopic examination of the urine. In this study, we aimed to examine whether vacuum aspiration of the urine sample has any adverse effect on sediment analysis by comparing results from vacuum and non-vacuum urine tubes. The study included totally 213 urine samples obtained from inpatients and outpatients in our hospital. Urine samples were collected to containers with aspiration system for vacuum tubes. Each sample was aliquoted to both vacuum and non-vacuum urine tubes. Urinary sediment analysis was performed using manual microscope. Results were evaluated using chi-square test. Comparison of the sediment analysis results from vacuum and non-vacuum urine tubes showed that results were highly concordant for erythrocyte, leukocyte and epithelial cells (gamma values 1, 0.997, and 0.994, respectively; p < .001). Results were also concordant for urinary casts, crystals and yeast (kappa values 0.815, 0.945 and 1, respectively; p < .001). The results show that in urinary sediment analysis, vacuum aspiration has no adverse effect on the cellular components except on casts.

Achieving 'excellent' indoor air quality in commercial offices equipped with air-handling unit--respirable suspended particulate.

PubMed

Lam, K S; Chan, F S; Fung, W Y; Lui, B S S; Lau, L W L

2006-04-01

A study was carried out to investigate the feasibility of achieving ultra low respirable suspended particulates (RSP) in commercial offices without major modification of existing ventilation systems by enhancing the particulates removal efficiency of existing central ventilation systems. Four types of filters which include pre-filters, cartridge filters, bag filters and high efficiency particulates air (HEPA) filters were tested in a commercial building in Causeway Bay. The results show that an RSP objective of <20 microg/m3 could be met by removing RSP from both the return air and outdoor air supply simultaneously. This level of performance is classed as 'excellent' by the Hong Kong Government, Environmental Protection Department. Filters with efficiency that exceed 80% placed both in the return air and outdoor air were sufficient to meet the objective. It is not necessary to install HEPA filters to achieve the 'excellent' class. The outdoor air filter has great influence on the steady state indoor RSP concentration while the effective cleaning rate is governed by the return air filter. Higher efficiency filters increased the static drop but the volume flow of the air fan was not affected significantly. The additional cost incurred was <5% of the existing operation cost. This paper reports a field study of RSP control for an indoor office environment. The results are directly applicable to building service engineering in the design of ventilation systems using air-handling units. Field observations indicated that indoor RSP in an office environment could be suppressed below 20 microg/m3 within 1 h by the simultaneous filtration of outdoor air and return air. Outdoor air filtration has a great influence on the steady state indoor concentration and return air filtration governs the cleaning rate. It is believed that the results of this study could be extended to the cleaning of other indoor pollutants such as volatile organic compounds.

Whole house particle removal and clean air delivery rates for in-duct and portable ventilation systems.

PubMed

Macintosh, David L; Myatt, Theodore A; Ludwig, Jerry F; Baker, Brian J; Suh, Helen H; Spengler, John D

2008-11-01

A novel method for determining whole house particle removal and clean air delivery rates attributable to central and portable ventilation/air cleaning systems is described. The method is used to characterize total and air-cleaner-specific particle removal rates during operation of four in-duct air cleaners and two portable air-cleaning devices in a fully instrumented test home. Operation of in-duct and portable air cleaners typically increased particle removal rates over the baseline rates determined in the absence of operating a central fan or an indoor air cleaner. Removal rates of 0.3- to 0.5-microm particles ranged from 1.5 hr(-1) during operation of an in-duct, 5-in. pleated media filter to 7.2 hr(-1) for an in-duct electrostatic air cleaner in comparison to a baseline rate of 0 hr(-1) when the air handler was operating without a filter. Removal rates for total particulate matter less than 2.5 microm in aerodynamic diameter (PM2.5) mass concentrations were 0.5 hr(-1) under baseline conditions, 0.5 hr(-1) during operation of three portable ionic air cleaners, 1 hr(-1) for an in-duct 1-in. media filter, 2.4 hr(-1) for a single high-efficiency particle arrestance (HEPA) portable air cleaner, 4.6 hr(-1) for an in-duct 5-in. media filter, 4.7 hr(-1) during operation of five portable HEPA filters, 6.1 hr(-1) for a conventional in-duct electronic air cleaner, and 7.5 hr(-1) for a high efficiency in-duct electrostatic air cleaner. Corresponding whole house clean air delivery rates for PM2.5 attributable to the air cleaner independent of losses within the central ventilation system ranged from 2 m3/min for the conventional media filter to 32 m3/min for the high efficiency in-duct electrostatic device. Except for the portable ionic air cleaner, the devices considered here increased particle removal indoors over baseline deposition rates.

Maximizing noise energy for noise-masking studies.

PubMed

Jules Étienne, Cédric; Arleo, Angelo; Allard, Rémy

2017-08-01

Noise-masking experiments are widely used to investigate visual functions. To be useful, noise generally needs to be strong enough to noticeably impair performance, but under some conditions, noise does not impair performance even when its contrast approaches the maximal displayable limit of 100 %. To extend the usefulness of noise-masking paradigms over a wider range of conditions, the present study developed a noise with great masking strength. There are two typical ways of increasing masking strength without exceeding the limited contrast range: use binary noise instead of Gaussian noise or filter out frequencies that are not relevant to the task (i.e., which can be removed without affecting performance). The present study combined these two approaches to further increase masking strength. We show that binarizing the noise after the filtering process substantially increases the energy at frequencies within the pass-band of the filter given equated total contrast ranges. A validation experiment showed that similar performances were obtained using binarized-filtered noise and filtered noise (given equated noise energy at the frequencies within the pass-band) suggesting that the binarization operation, which substantially reduced the contrast range, had no significant impact on performance. We conclude that binarized-filtered noise (and more generally, truncated-filtered noise) can substantially increase the energy of the noise at frequencies within the pass-band. Thus, given a limited contrast range, binarized-filtered noise can display higher energy levels than Gaussian noise and thereby widen the range of conditions over which noise-masking paradigms can be useful.

Loading and unloading of freeze-dryers: airborne contamination risks for aseptically manufactured sterile drug products.

PubMed

Ljungqvist, Bengt; Reinmüller, Berit

2007-01-01

In pharmaceutical manufacturing, freeze-drying processes can be adversely affected by temperature differences relative to the surrounding air. Loading and unloading of freeze-dryers are performed either without or with temperature differences between the cleanroom and the chamber of the freeze-dryer. This operation can cause a flow of room air through the opening, creating a contamination risk, especially when manual handling of material is performed in this area. To minimize this risk, a high-efficiency particulate air (HEPA) filter unit should be installed above the opening to provide clean air and protect the opening. Here the theoretical relationships are discussed and design criteria are presented.

49 CFR 572.189 - Instrumentation and test conditions.

Code of Federal Regulations, 2014 CFR

2014-10-01

.... The sum mass of the attachments and 1/3 cable mass must not exceed 5 percent of the total pendulum... filtered CFC 180; (3)Neck and lumbar spine pendulum accelerations—Digitally filtered CFC 60; (4) Pelvis... 180. (j)(1) Filter the pendulum acceleration data using a SAE J211 CFC 60 filter. (2) Determine the...

Mitigation of radon and thoron decay products by filtration.

PubMed

Wang, Jin; Meisenberg, Oliver; Chen, Yongheng; Karg, Erwin; Tschiersch, Jochen

2011-09-01

Inhalation of indoor radon ((222)Rn) and thoron ((220)Rn) decay products is the most important source of exposure to ionizing radiation for the human respiratory tract. Decreasing ventilation rates due to energy saving reasons in new buildings suggest additional active mitigation techniques to reduce the exposure in homes with high radon and thoron concentrations but poor ventilation. Filtration techniques with HEPA filters and simple surgical mask material have been tested for their potential to reduce the indoor exposure in terms of the total effective dose for mixed radon and thoron indoor atmospheres. The tests were performed inside an experimental room providing stable conditions. Filtration (at filtration rates of 0.2 h(-1) and larger) removes attached radon and thoron decay products effectively but indoor aerosol as well. Therefore the concentration of unattached decay products (which have a higher dose coefficient) may increase. The decrease of the attached decay product concentrations could be theoretically described by a slowly decreasing exponential process. For attached radon decay products, it exhibited a faster but weaker removal process compared to attached thoron decay products (-70% for attached radon decay products and -80% for attached thoron decay products at a filtration rate of 0.5 h(-1) with an HEPA filter). The concentration of unattached thoron decay products increased distinctly during the filtration process (+300%) while that of unattached radon decay products rose only slightly though at a much higher level (+17%). In the theoretical description these observed differences could be attributed to the different half-lives of the nuclides. Considering both effects, reduced attached and increased unattached decay product concentrations, filtration could significantly decrease the total effective dose from thoron whereas the overall effect on radon dose is small. A permanent filtration is recommended because of the slow decrease of the thoron decay product concentrations. Copyright © 2011 Elsevier B.V. All rights reserved.

Force reflection with compliance control

NASA Technical Reports Server (NTRS)

Kim, Won S. (Inventor)

1993-01-01

Two types of systems for force-reflecting control, which enables high force-reflection gain, are presented: position-error-based force reflection and low-pass-filtered force reflection. Both of the systems are combined with shared compliance control. In the position-error-based class, the position error between the commanded and the actual position of a compliantly controlled robot is used to provide force reflection. In the low-pass-filtered force reflection class, the low-pass-filtered output of the compliance control is used to provide force reflection. The increase in force reflection gain can be more than 10-fold as compared to a conventional high-bandwidth pure force reflection system, when high compliance values are used for the compliance control.

Mode-filtered large-core fiber for optical coherence tomography

PubMed Central

Moon, Sucbei; Chen, Zhongping

2013-01-01

We have investigated the use of multimode fiber in optical coherence tomography (OCT) with a mode filter that selectively suppresses the power of the high-order modes (HOMs). A large-core fiber (LCF) that has a moderate number of guiding modes was found to be an attractive alternative to the conventional single-mode fiber for its large mode area and the consequentially wide Rayleigh range of the output beam if the HOMs of the LCF were efficiently filtered out by a mode filter installed in the middle. For this, a simple mode filtering scheme of a fiber-coil mode filter was developed in this study. The LCF was uniformly coiled by an optimal bend radius with a fiber winder, specially devised for making a low-loss mode filter. The feasibility of the mode-filtered LCF in OCT imaging was tested with a common-path OCT system. It has been successfully demonstrated that our mode-filtered LCF can provide a useful imaging or sensing probe without an objective lens that greatly simplifies the structure of the probing optics. PMID:23207399

Comparison of vacuum rise time, vacuum limit accuracy, and occlusion break surge of 3 new phacoemulsification systems.

PubMed

Han, Young Keun; Miller, Kevin M

2009-08-01

To compare vacuum rise time, vacuum limit accuracy, and occlusion break surge of 3 new phacoemulsification machines. Jules Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, California, USA. The vacuum rise time under normal and enhanced aspiration modes, vacuum limit accuracy, and occlusion break surge of the Infiniti Vision System, Stellaris Vision Enhancement System, and WhiteStar Signature Phacoemulsification System were tested. Vacuum rise time and limit accuracy were measured at limit settings of 400 mm Hg and 600 mm Hg. Surge area was recorded at vacuum limit settings of 200 mm Hg, 300 mm Hg, 400 mm Hg, and 500 mm Hg. The Infiniti had the fastest vacuum rise times under normal and enhanced aspiration modes. At 4 seconds, the vacuum limit accuracy was greatest with the Infiniti at the 400 mm Hg limit and the Signature at the 600 mm Hg limit. The Stellaris did not reach either vacuum target. The Infiniti performed better than the other 2 machines during testing of occlusion break surge at all vacuum limit settings above 200 mm Hg. Under controlled laboratory test conditions, the Infiniti had the fastest vacuum rise time, greatest vacuum limit accuracy at 400 mm Hg, and least occlusion break surge. These results can be explained by the lower compliance of the Infiniti system.

Knowledge-based iterative model reconstruction: comparative image quality and radiation dose with a pediatric computed tomography phantom.

PubMed

Ryu, Young Jin; Choi, Young Hun; Cheon, Jung-Eun; Ha, Seongmin; Kim, Woo Sun; Kim, In-One

2016-03-01

CT of pediatric phantoms can provide useful guidance to the optimization of knowledge-based iterative reconstruction CT. To compare radiation dose and image quality of CT images obtained at different radiation doses reconstructed with knowledge-based iterative reconstruction, hybrid iterative reconstruction and filtered back-projection. We scanned a 5-year anthropomorphic phantom at seven levels of radiation. We then reconstructed CT data with knowledge-based iterative reconstruction (iterative model reconstruction [IMR] levels 1, 2 and 3; Philips Healthcare, Andover, MA), hybrid iterative reconstruction (iDose(4), levels 3 and 7; Philips Healthcare, Andover, MA) and filtered back-projection. The noise, signal-to-noise ratio and contrast-to-noise ratio were calculated. We evaluated low-contrast resolutions and detectability by low-contrast targets and subjective and objective spatial resolutions by the line pairs and wire. With radiation at 100 peak kVp and 100 mAs (3.64 mSv), the relative doses ranged from 5% (0.19 mSv) to 150% (5.46 mSv). Lower noise and higher signal-to-noise, contrast-to-noise and objective spatial resolution were generally achieved in ascending order of filtered back-projection, iDose(4) levels 3 and 7, and IMR levels 1, 2 and 3, at all radiation dose levels. Compared with filtered back-projection at 100% dose, similar noise levels were obtained on IMR level 2 images at 24% dose and iDose(4) level 3 images at 50% dose, respectively. Regarding low-contrast resolution, low-contrast detectability and objective spatial resolution, IMR level 2 images at 24% dose showed comparable image quality with filtered back-projection at 100% dose. Subjective spatial resolution was not greatly affected by reconstruction algorithm. Reduced-dose IMR obtained at 0.92 mSv (24%) showed similar image quality to routine-dose filtered back-projection obtained at 3.64 mSv (100%), and half-dose iDose(4) obtained at 1.81 mSv.

Coffee consumption and the risk of malignant melanoma in the Norwegian Women and Cancer (NOWAC) Study.

PubMed

Lukic, Marko; Jareid, Mie; Weiderpass, Elisabete; Braaten, Tonje

2016-07-29

Coffee contains biologically-active substances that suppress carcinogenesis in vivo, and coffee consumption has been associated with a lower risk of malignant melanoma. We studied the impact of total coffee consumption and of different brewing methods on the incidence of malignant melanoma in a prospective cohort of Norwegian women. We had baseline information on total coffee consumption and consumption of filtered, instant, and boiled coffee from self-administered questionnaires for 104,080 women in the Norwegian Women and Cancer (NOWAC) Study. We also had follow-up information collected 6-8 years after baseline. Multiple imputation was used to deal with missing data, and multivariable Cox regression models were used to calculate hazard ratios (HR) for malignant melanoma by consumption category of total, filtered, instant, and boiled coffee. During 1.7 million person-years of follow-up, 762 cases of malignant melanoma were diagnosed. Compared to light consumers of filtered coffee (≤1 cup/day), we found a statistically significant inverse association with low-moderate consumption (>1-3 cups/day, HR = 0.80; 95 % confidence interval [CI] 0.66-0.98) and high-moderate consumption of filtered coffee (>3-5 cups/day, HR = 0.77; 95 % CI 0.61-0.97) and melanoma risk (p trend = 0.02). We did not find a statistically significant association between total, instant, or boiled coffee consumption and the risk of malignant melanoma in any of the consumption categories. The data from the NOWAC Study indicate that a moderate intake of filtered coffee could reduce the risk of malignant melanoma.

Sugar maple sap volume increases as vacuum level is increased

Treesearch

Russell S. Walters; H. Clay Smith

1975-01-01

Maple sap yields collected by using plastic tubing with a vacuum pump increased as the vacuum level was increased. Sap volumes collected at the 10- and 15-inch mercury vacuum levels were statistically significantly higher than volumes collected at the 5-inch level. Although the 15-inch vacuum yielded more sap than the 10-inch vacuum, the difference was not...

Comparing Nutrient Removal from Membrane Filtered and Unfiltered Domestic Wastewater Using Chlorella vulgaris

PubMed Central

Mayhead, Elyssia; Llewellyn, Carole A.; Fuentes-Grünewald, Claudio

2018-01-01

The nutrient removal efficiency of Chlorella vulgaris cultivated in domestic wastewater was investigated, along with the potential to use membrane filtration as a pre-treatment tool during the wastewater treatment process. Chlorella vulgaris was batch cultivated for 12 days in a bubble column system with two different wastewater treatments. Maximum uptake of 94.18% ammonium (NH4-N) and 97.69% ortho-phosphate (PO4-P) occurred in 0.2 μm membrane filtered primary wastewater. Membrane filtration enhanced the nutrient uptake performance of C. vulgaris by removing bacteria, protozoa, colloidal particles and suspended solids, thereby improving light availability for photosynthesis. The results of this study suggest that growing C. vulgaris in nutrient rich membrane filtered wastewater provides an option for domestic wastewater treatment to improve the quality of the final effluent. PMID:29351200

Vacuum-Gauge Connection For Shipping Container

NASA Technical Reports Server (NTRS)

Henry, Robert H.

1990-01-01

External connector enables measurement of vacuum in stored part. Remote-readout connector added to shipping container and connected to thermo-couple vacuum gauge in vacuum-insulated cryogenic line packed in container. Enables monitoring of condition of vacuum without opening container.

Activity-guided isolation of the chemical constituents of Muntingia calabura using a quinone reductase induction assay.

PubMed

Su, Bao-Ning; Jung Park, Eun; Vigo, Jose Schunke; Graham, James G; Cabieses, Fernando; Fong, Harry H S; Pezzuto, John M; Kinghorn, A Douglas

2003-06-01

Activity-guided fractionation of an EtOAc-soluble extract of the leaves of Muntingia calabura collected in Peru, using an in vitro quinone reductase induction assay with cultured Hepa 1c1c7 (mouse hepatoma) cells, resulted in the isolation of a flavanone with an unsubstituted B-ring, (2R,3R)-7-methoxy-3,5,8-trihydroxyflavanone (5), as well as 24 known compounds, which were mainly flavanones and flavones. The structure including absolute stereochemistry of compound 5 was determined by spectroscopic (HRMS, 1D and 2D NMR, and CD spectra) methods. Of the isolates obtained, in addition to 5, (2S)-5-hydroxy-7-methoxyflavanone, 2',4'-dihydroxychalcone, 4,2',4'-trihydroxychalcone, 7-hydroxyisoflavone and 7,3',4'-trimethoxyisoflavone were found to induce quinone reductase activity.

Mesoporous Akaganeite of Adjustable Pore Size Synthesized using Mixed Templates

NASA Astrophysics Data System (ADS)

Zhang, Y.; Ge, D. L.; Ren, H. P.; Fan, Y. J.; Wu, L. M.; Sun, Z. X.

2017-12-01

Mesoporous akaganeite with large and adjustable pore size was synthesized through a co-template method, which was achieved by the combined interaction between PEG2000 and alkyl amines with different lengths of the straight carbon chain. The characterized results indicate that the synthesized samples show comparatively narrow BJH pore size distributions and centered at 14.3 nm when PEG and HEPA was used, and it could be enlarged to 16.8 and 19.4 nm respectively through changing the alkyl amines to DDA and HDA. Meanwhile, all the synthesized akaganeite possess relativity high specific surface area ranging from 183 to 281 m2/g and high total pore volume of 0.98 to 1.5 cm3/g. A possible mechanism leading to the pore size changing was also proposed.

High-resolution low-frequency fluctuation map of a multimode laser diode subject to filtered optical feedback via a fiber Bragg grating.

PubMed

Baladi, Fadwa; Lee, Min Won; Burie, Jean-René; Bettiati, Mauro A; Boudrioua, Azzedine; Fischer, Alexis P A

2016-07-01

A highly detailed and extended map of low-frequency fluctuations is established for a high-power multi-mode 980 nm laser diode subject to filtered optical feedback from a fiber Bragg grating. The low-frequency fluctuations limits and substructures exhibit substantial differences with previous works.

Thermodynamic performance of multi-stage gradational lead screw vacuum pump

NASA Astrophysics Data System (ADS)

Zhao, Fan; Zhang, Shiwei; Sun, Kun; Zhang, Zhijun

2018-02-01

As a kind of dry mechanical vacuum pump, the twin-screw vacuum pump has an outstanding pumping performance during operation, widely used in the semiconductor industry. Compared with the constant lead screw (CLS) vacuum pump, the gradational lead screw (GLS) vacuum pump is more popularly applied in recent years. Nevertheless, not many comparative studies on the thermodynamic performance of GLS vacuum pump can be found in the literature. Our study focuses on one type of GLS vacuum pump, the multi-stage gradational lead screw (MGLS) vacuum pump, gives a detailed description of its construction and illustrates it with the drawing. Based on the structural analysis, the thermodynamic procedure is divided into four distinctive processes, including sucking process, transferring (compressing) process, backlashing process and exhausting process. The internal mechanism of each process is qualitatively illustrated and the mathematical expressions of seven thermodynamic parameters are given under the ideal situation. The performance curves of MGLS vacuum pump are plotted by MATLAB software and compared with those of the CLS vacuum pump in the same case. The results can well explain why the MGLS vacuum pump has more favorable pumping performance than the CLS vacuum pump in saving energy, reducing noise and heat dissipation.

Natural vacuum electronics

NASA Technical Reports Server (NTRS)

Leggett, Nickolaus

1990-01-01

The ambient natural vacuum of space is proposed as a basis for electron valves. Each valve is an electron controlling structure similiar to a vacuum tube that is operated without a vacuum sustaining envelope. The natural vacuum electron valves discussed offer a viable substitute for solid state devices. The natural vacuum valve is highly resistant to ionizing radiation, system generated electromagnetic pulse, current transients, and direct exposure to space conditions.

Sources and sinks of filtered total mercury and concentrations of total mercury of solids and of filtered methylmercury, Sinclair Inlet, Kitsap County, Washington, 2007-10

USGS Publications Warehouse

Paulson, Anthony J.; Dinicola, Richard S.; Noble, Marlene A.; Wagner, Richard J.; Huffman, Raegan L.; Moran, Patrick W.; DeWild, John F.

2012-01-01

The majority of filtered total mercury in the marine water of Sinclair Inlet originates from salt water flowing from Puget Sound. About 420 grams of filtered total mercury are added to Sinclair Inlet each year from atmospheric, terrestrial, and sedimentary sources, which has increased filtered total mercury concentrations in Sinclair Inlet (0.33 nanograms per liter) to concentrations greater than those of the Puget Sound (0.2 nanograms per liter). The category with the largest loading of filtered total mercury to Sinclair Inlet included diffusion of porewaters from marine sediment to the water column of Sinclair Inlet and discharge through the largest stormwater drain on the Bremerton naval complex, Bremerton, Washington. However, few data are available to estimate porewater and stormwater releases with any certainty. The release from the stormwater drain does not originate from overland flow of stormwater. Rather total mercury on soils is extracted by the chloride ions in seawater as the stormwater is drained and adjacent soils are flushed with seawater by tidal pumping. Filtered total mercury released by an unknown freshwater mechanism also was observed in the stormwater flowing through this drain. Direct atmospheric deposition on the Sinclair Inlet, freshwater discharge from creek and stormwater basins draining into Sinclair Inlet, and saline discharges from the dry dock sumps of the naval complex are included in the next largest loading category of sources of filtered total mercury. Individual discharges from a municipal wastewater treatment plant and from the industrial steam plant of the naval complex constituted the loading category with the third largest loadings. Stormwater discharge from the shipyard portion of the naval complex and groundwater discharge from the base are included in the loading category with the smallest loading of filtered total mercury. Presently, the origins of the solids depositing to the sediment of Sinclair Inlet are uncertain, and consequently, concentrations of sediments can be qualitatively compared only to total mercury concentrations of solids suspended in the water column. Concentrations of total mercury of suspended solids from creeks, stormwater, and even wastewater effluent discharging into greater Sinclair Inlet were comparable to concentrations of solids suspended in the water column of Sinclair Inlet. Concentrations of total mercury of suspended solids were significantly lower than those of marine bed sediment of Sinclair Inlet; these suspended solids have been shown to settle in Sinclair Inlet. The settling of suspended solids in the greater Sinclair Inlet and in Operable Unit B Marine of the naval complex likely will result in lower concentrations of total mercury in sediments. Such a decrease in total mercury concentrations was observed in the sediment of Operable Unit B Marine in 2010. However, total mercury concentrations of solids discharged from several sources from the Bremerton naval complex were higher than concentrations in sediment collected from Operable Unit B Marine. The combined loading of solids from these sources is small compared to the amount of solids depositing in OU B Marine. However, total mercury concentration in sediment collected at a monitoring station just offshore one of these sources, the largest stormwater drain on the Bremerton naval complex, increased considerably in 2010. Low methylmercury concentrations were detected in groundwater, stormwater, and effluents discharged from the Bremerton naval complex. The highest methylmercury concentrations were measured in the porewaters of highly reducing marine sediment in greater Sinclair Inlet. The marine sediment collected off the largest stormwater drain contained low concentrations of methylmercury in porewater because these sediments were not highly reducing.

Investigation of Total and Hexavalent Chromium in Filtered and Unfiltered Groundwater Samples at the Tucson International Airport Superfund Site.

PubMed

Tillman, Fred D; McCleskey, R Blaine; Hermosillo, Edyth

2016-10-01

Potential health effects from hexavalent chromium in groundwater have recently become a concern to regulators at the Tucson International Airport Area Superfund site. In 2016, the U.S. Geological Survey sampled 46 wells in the area to characterize the nature and extent of chromium in groundwater, to understand what proportion of total chromium is in the hexavalent state, and to determine if substantial differences are present between filtered and unfiltered chromium concentrations. Results indicate detectable chromium concentrations in all wells, over 75 % of total chromium is in the hexavalent state in a majority of wells, and filtered and unfiltered results differ substantially in only a few high-turbidity total chromium samples.

The Filtered Words Test and the Influence of Lexicality

ERIC Educational Resources Information Center

Arnott, Wendy; Goli, Tara; Bradley, Andrew; Smith, Andrew; Wilson, Wayne

2014-01-01

Purpose: In the present study, the authors aimed to investigate the language confounds of filtered words tests by examining the repetition of real words versus nonsense words as a function of level of filtering. Method: Fifty-five young, native-English-speaking women with normal hearing were required to repeat 80 real-word and 80 nonsense-word…

Edge-based correlation image registration for multispectral imaging

DOEpatents

Nandy, Prabal [Albuquerque, NM

2009-11-17

Registration information for images of a common target obtained from a plurality of different spectral bands can be obtained by combining edge detection and phase correlation. The images are edge-filtered, and pairs of the edge-filtered images are then phase correlated to produce phase correlation images. The registration information can be determined based on these phase correlation images.

Electroweak vacuum instability and renormalized Higgs field vacuum fluctuations in the inflationary universe

NASA Astrophysics Data System (ADS)

Kohri, Kazunori; Matsui, Hiroki

2017-08-01

In this work, we investigated the electroweak vacuum instability during or after inflation. In the inflationary Universe, i.e., de Sitter space, the vacuum field fluctuations < δ phi 2 > enlarge in proportion to the Hubble scale H2. Therefore, the large inflationary vacuum fluctuations of the Higgs field < δ phi 2 > are potentially catastrophic to trigger the vacuum transition to the negative-energy Planck-scale vacuum state and cause an immediate collapse of the Universe. However, the vacuum field fluctuations < δ phi 2 >, i.e., the vacuum expectation values have an ultraviolet divergence, and therefore a renormalization is necessary to estimate the physical effects of the vacuum transition. Thus, in this paper, we revisit the electroweak vacuum instability from the perspective of quantum field theory (QFT) in curved space-time, and discuss the dynamical behavior of the homogeneous Higgs field phi determined by the effective potential V eff( phi ) in curved space-time and the renormalized vacuum fluctuations < δ phi 2 >ren via adiabatic regularization and point-splitting regularization. We simply suppose that the Higgs field only couples the gravity via the non-minimal Higgs-gravity coupling ξ(μ). In this scenario, the electroweak vacuum stability is inevitably threatened by the dynamical behavior of the homogeneous Higgs field phi, or the formations of AdS domains or bubbles unless the Hubble scale is small enough H< ΛI .

7 CFR 319.8-10 - Covers.

Code of Federal Regulations, 2014 CFR

2014-01-01

... vacuum fumigation by an approved method or without vacuum fumigation when the covers are to be moved to... northern port for vacuum fumigation thereat or for movement to a mill or plant for utilization such... California port where approved vacuum fumigation facilities are available for vacuum fumigation thereat by an...

7 CFR 305.29 - Vacuum heat treatment schedule.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 5 2010-01-01 2010-01-01 false Vacuum heat treatment schedule. 305.29 Section 305.29... SERVICE, DEPARTMENT OF AGRICULTURE PHYTOSANITARY TREATMENTS Heat Treatments § 305.29 Vacuum heat treatment... vacuum at 8 hours. Maintain the vacuum until the end of the treatment. Gradually increase the temperature...

7 CFR 319.8-10 - Covers.

Code of Federal Regulations, 2012 CFR

2012-01-01

... vacuum fumigation by an approved method or without vacuum fumigation when the covers are to be moved to... northern port for vacuum fumigation thereat or for movement to a mill or plant for utilization such... California port where approved vacuum fumigation facilities are available for vacuum fumigation thereat by an...

Development of High Interruption Capability Vacuum Circuit Breaker -Technology of Vacuum Arc Control-

NASA Astrophysics Data System (ADS)

Niwa, Yoshimitsu; Kaneko, Eiji

Vacuum circuit breakers (VCB) have been widely used for power distribution systems. Vacuum Interrupters, which are the current interruption unit, have been increased its interruption capability with the development of vacuum arc control technology by magnetic field. There are three major type electrodes: disk shaped electrodes, radial magnetic field electrodes, axial magnetic field (AMF) electrodes. In the disk shaped electrode, the vacuum arc between the electrodes is not controlled. In the AMF electrode, the vacuum arc is diffused and stabilized by an axial magnetic field, which is parallel to the arc current. In the last type of electrodes, the vacuum arc column is rotated by magnetic force generated by the current flowing in the electrodes. The interruption current and the voltage of one break VCB is increased to 100 kA, 144 kV respectively. This paper describes basic configurations and functions of VCB, vacuum arc control technology in vacuum interrupters, recent researches and applications of VCB.

Investigation of total and hexavalent chromium in filtered and unfiltered groundwater samples at the Tucson International Airport Superfund Site

USGS Publications Warehouse

Tillman, Fred; McCleskey, R. Blaine; Hermosillo, Edyth

2016-01-01

Potential health effects from hexavalent chromium in groundwater have recently become a concern to regulators at the Tucson International Airport Area Superfund site. In 2016, the U.S. Geological Survey sampled 46 wells in the area to characterize the nature and extent of chromium in groundwater, to understand what proportion of total chromium is in the hexavalent state, and to determine if substantial differences are present between filtered and unfiltered chromium concentrations. Results indicate detectable chromium concentrations in all wells, over 75 % of total chromium is in the hexavalent state in a majority of wells, and filtered and unfiltered results differ substantially in only a few high-turbidity total chromium samples.

Short-range/Long-range Integrated Target (SLIT) for Video Guidance Sensor Rendezvous and Docking

NASA Technical Reports Server (NTRS)

Roe, Fred D. (Inventor); Bryan, Thomas C. (Inventor)

2009-01-01

A laser target reflector assembly for mounting upon spacecraft having a long-range reflector array formed from a plurality of unfiltered light reflectors embedded in an array pattern upon a hemispherical reflector disposed upon a mounting plate. The reflector assembly also includes a short-range reflector array positioned upon the mounting body proximate to the long-range reflector array. The short-range reflector array includes three filtered light reflectors positioned upon extensions from the mounting body. The three filtered light reflectors retro-reflect substantially all incident light rays that are transmissive by their monochromatic filters and received by the three filtered light reflectors. In one embodiment the short-range reflector array is embedded within the hemispherical reflector,

7 CFR 319.8-10 - Covers.

Code of Federal Regulations, 2011 CFR

2011-01-01

... vacuum fumigation by an approved method or without vacuum fumigation when the covers are to be moved to... northern port for vacuum fumigation thereat or for movement to a mill or plant for utilization such... California port where approved vacuum fumigation facilities are available for vacuum fumigation thereat by an...

7 CFR 319.8-10 - Covers.

Code of Federal Regulations, 2013 CFR

2013-01-01

... vacuum fumigation by an approved method or without vacuum fumigation when the covers are to be moved to... northern port for vacuum fumigation thereat or for movement to a mill or plant for utilization such... California port where approved vacuum fumigation facilities are available for vacuum fumigation thereat by an...

21 CFR 864.9125 - Vacuum-assisted blood collection system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Vacuum-assisted blood collection system. 864.9125... Blood and Blood Products § 864.9125 Vacuum-assisted blood collection system. (a) Identification. A vacuum-assisted blood collection system is a device intended for medical purposes that uses a vacuum to...

21 CFR 864.9125 - Vacuum-assisted blood collection system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Vacuum-assisted blood collection system. 864.9125... Blood and Blood Products § 864.9125 Vacuum-assisted blood collection system. (a) Identification. A vacuum-assisted blood collection system is a device intended for medical purposes that uses a vacuum to...

21 CFR 864.9125 - Vacuum-assisted blood collection system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Vacuum-assisted blood collection system. 864.9125... Blood and Blood Products § 864.9125 Vacuum-assisted blood collection system. (a) Identification. A vacuum-assisted blood collection system is a device intended for medical purposes that uses a vacuum to...

21 CFR 864.9125 - Vacuum-assisted blood collection system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Vacuum-assisted blood collection system. 864.9125... Blood and Blood Products § 864.9125 Vacuum-assisted blood collection system. (a) Identification. A vacuum-assisted blood collection system is a device intended for medical purposes that uses a vacuum to...

Germanium detector vacuum encapsulation

NASA Technical Reports Server (NTRS)

Madden, N. W.; Malone, D. F.; Pehl, R. H.; Cork, C. P.; Luke, P. N.; Landis, D. A.; Pollard, M. J.

1991-01-01

This paper describes an encapsulation technology that should significantly improve the viability of germanium gamma-ray detectors for a number of important applications. A specialized vacuum chamber has been constructed in which the detector and the encapsulating module are processed in high vacuum. Very high vacuum conductance is achieved within the valveless encapsulating module. The detector module is then sealed without breaking the chamber vacuum. The details of the vacuum chamber, valveless module, processing, and sealing method are presented.

Connecting blazars with ultrahigh-energy cosmic rays and astrophysical neutrinos

NASA Astrophysics Data System (ADS)

Resconi, E.; Coenders, S.; Padovani, P.; Giommi, P.; Caccianiga, L.

2017-06-01

We present a strong hint of a connection between high-energy γ-ray emitting blazars, very high energy neutrinos, and ultrahigh-energy cosmic rays. We first identify potential hadronic sources by filtering γ-ray emitters in spatial coincidence with the high-energy neutrinos detected by IceCube. The neutrino filtered γ-ray emitters are then correlated with the ultrahigh-energy cosmic rays from the Pierre Auger Observatory and the Telescope Array by scanning in γ-ray flux (Fγ) and angular separation (θ) between sources and cosmic rays. A maximal excess of 80 cosmic rays (42.5 expected) is found at θ ≤ 10° from the neutrino-filtered γ-ray emitters selected from the second hard Fermi-LAT catalogue (2FHL) and for Fγ(>50 GeV) ≥ 1.8 × 10-11 ph cm-2 s-1. The probability for this to happen is 2.4 × 10-5, which translates to ˜2.4 × 10-3 after compensation for all the considered trials. No excess of cosmic rays is instead observed for the complement sample of γ-ray emitters (I.e. not in spatial connection with IceCube neutrinos). A likelihood ratio test comparing the connection between the neutrino-filtered and the complement source samples with the cosmic rays favours a connection between neutrino-filtered emitters and cosmic rays with a probability of ˜1.8 × 10-3 (2.9σ) after compensation for all the considered trials. The neutrino-filtered γ-ray sources that make up the cosmic rays excess are blazars of the high synchrotron peak type. More statistics is needed to further investigate these sources as candidate cosmic ray and neutrino emitters.

In Situ Observation of Carbonaceous Material in the Matrices of CV and CM Carbonaceous Chondrites: Preliminary Results from Energy Filtered Transmission Electron Microscopy

NASA Technical Reports Server (NTRS)

Brearley, A. J.; Abreu, N. M.

2001-01-01

Energy filtered transmission electron microscopy shows that organic matter can be detected in situ in the matrices of carbonaceous chondrites at a spatial resolution of at least 1 nm. In CM chondrites, carbon is often associated with sulfide particles. Additional information is contained in the original extended abstract.

Heterogeneous Distribution of Carbonaceous Material in Murchison Matrix: In Situ Observations Using Energy Filtered Transmission Electron Microscopy

NASA Technical Reports Server (NTRS)

Brearley, Adrian J.

2002-01-01

Energy filtered TEM (Transmission Electron Microscopy) has been used to study the location of carbonaceous material in situ in Murchison matrix. Carbon occurs frequently as narrow rims around sulfide grains, but is rare in regions of matrix that are dominated by phyllosilicates. Additional information is contained in the original extended abstract.

Enhanced Photocatalytic Activity of Vacuum-activated TiO2 Induced by Oxygen Vacancies.

PubMed

Dong, Guoyan; Wang, Xin; Chen, Zhiwu; Lu, Zhenya

2018-05-01

TiO 2 (Degussa P25) photocatalysts harboring abundant oxygen vacancies (Vacuum P25) were manufactured using a simple and economic Vacuum deoxidation process. Control experiments showed that temperature and time of vacuum deoxidation had a significant effect on Vacuum P25 photocatalytic activity. After 240 min of visible light illumination, the optimal Vacuum P25 photocatalysts (vacuum deoxidation treated at 330 °C for 3 h) reach as high as 94% and 88% of photodegradation efficiency for rhodamine B (RhB) and tetracycline, respectively, which are around 4.5 and 4.9 times as that of pristine P25. The XPS, PL and EPR analyses indicated that the oxygen vacancies were produced in the Vacuum P25 during the vacuum deoxidation process. The oxygen vacancy states can produce vacancy energy level located below the conduction band minimum, which resulting in the bandgap narrowing, thus extending the photoresponse wavelength range of Vacuum P25. The positron annihilation analysis indicated that the concentrations ratio of bulk and surface oxygen vacancies could be adjusted by changing the vacuum deoxidation temperature and time. Decreasing the ratio of bulk and surface oxygen vacancies was shown to improve photogenerated electron-hole pair separation efficiency, which leads to an obvious enhancement of the visible photocatalytic activities of Vacuum P25. © 2017 The American Society of Photobiology.

Demonstrations with a Vacuum: Old Demonstrations for New Vacuum Pumps.

ERIC Educational Resources Information Center

Greenslade, Thomas B., Jr.

1989-01-01

Explains mechanisms of 19th-century vacuum pumps. Describes demonstrations using the pump including guinea and feather tube, aurora tube, electric egg, Gassiots cascade, air mill, bell in vacuum, density and buoyancy of air, fountain in vacuum, mercury shower, palm and bladder glasses, Bacchus demonstration, pneumatic man-lifter, and Magdeburg…

21 CFR 864.9125 - Vacuum-assisted blood collection system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Vacuum-assisted blood collection system. 864.9125... Blood and Blood Products § 864.9125 Vacuum-assisted blood collection system. (a) Identification. A vacuum-assisted blood collection system is a device intended for medical purposes that uses a vacuum to...

Development of Filtered Rayleigh Scattering for Accurate Measurement of Gas Velocity

NASA Technical Reports Server (NTRS)

Miles, Richard B.; Lempert, Walter R.

1995-01-01

The overall goals of this research were to develop new diagnostic tools capable of capturing unsteady and/or time-evolving, high-speed flow phenomena. The program centers around the development of Filtered Rayleigh Scattering (FRS) for velocity, temperature, and density measurement, and the construction of narrow linewidth laser sources which will be capable of producing an order MHz repetition rate 'burst' of high power pulses.

Electroweak vacuum instability and renormalized Higgs field vacuum fluctuations in the inflationary universe

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kohri, Kazunori; Matsui, Hiroki, E-mail: kohri@post.kek.jp, E-mail: matshiro@post.kek.jp

In this work, we investigated the electroweak vacuum instability during or after inflation. In the inflationary Universe, i.e., de Sitter space, the vacuum field fluctuations < δ φ {sup 2} > enlarge in proportion to the Hubble scale H {sup 2}. Therefore, the large inflationary vacuum fluctuations of the Higgs field < δ φ {sup 2} > are potentially catastrophic to trigger the vacuum transition to the negative-energy Planck-scale vacuum state and cause an immediate collapse of the Universe. However, the vacuum field fluctuations < δ φ {sup 2} >, i.e., the vacuum expectation values have an ultraviolet divergence, andmore » therefore a renormalization is necessary to estimate the physical effects of the vacuum transition. Thus, in this paper, we revisit the electroweak vacuum instability from the perspective of quantum field theory (QFT) in curved space-time, and discuss the dynamical behavior of the homogeneous Higgs field φ determined by the effective potential V {sub eff}( φ ) in curved space-time and the renormalized vacuum fluctuations < δ φ {sup 2} >{sub ren} via adiabatic regularization and point-splitting regularization. We simply suppose that the Higgs field only couples the gravity via the non-minimal Higgs-gravity coupling ξ(μ). In this scenario, the electroweak vacuum stability is inevitably threatened by the dynamical behavior of the homogeneous Higgs field φ, or the formations of AdS domains or bubbles unless the Hubble scale is small enough H < Λ {sub I} .« less

Three dimensional empirical mode decomposition analysis apparatus, method and article manufacture

NASA Technical Reports Server (NTRS)

Gloersen, Per (Inventor)

2004-01-01

An apparatus and method of analysis for three-dimensional (3D) physical phenomena. The physical phenomena may include any varying 3D phenomena such as time varying polar ice flows. A repesentation of the 3D phenomena is passed through a Hilbert transform to convert the data into complex form. A spatial variable is separated from the complex representation by producing a time based covariance matrix. The temporal parts of the principal components are produced by applying Singular Value Decomposition (SVD). Based on the rapidity with which the eigenvalues decay, the first 3-10 complex principal components (CPC) are selected for Empirical Mode Decomposition into intrinsic modes. The intrinsic modes produced are filtered in order to reconstruct the spatial part of the CPC. Finally, a filtered time series may be reconstructed from the first 3-10 filtered complex principal components.

Introducing the Filtered Park's and Filtered Extended Park's Vector Approach to detect broken rotor bars in induction motors independently from the rotor slots number

NASA Astrophysics Data System (ADS)

Gyftakis, Konstantinos N.; Marques Cardoso, Antonio J.; Antonino-Daviu, Jose A.

2017-09-01

The Park's Vector Approach (PVA), together with its variations, has been one of the most widespread diagnostic methods for electrical machines and drives. Regarding the broken rotor bars fault diagnosis in induction motors, the common practice is to rely on the width increase of the Park's Vector (PV) ring and then apply some more sophisticated signal processing methods. It is shown in this paper that this method can be unreliable and is strongly dependent on the magnetic poles and rotor slot numbers. To overcome this constraint, the novel Filtered Park's/Extended Park's Vector Approach (FPVA/FEPVA) is introduced. The investigation is carried out with FEM simulations and experimental testing. The results prove to satisfyingly coincide, whereas the proposed advanced FPVA method is desirably reliable.

Bottled, filtered, and tap water use in Latino and non-Latino children.

PubMed

Hobson, Wendy L; Knochel, Miguel L; Byington, Carrie L; Young, Paul C; Hoff, Charles J; Buchi, Karen F

2007-05-01

To describe bottled, filtered, and tap water consumption and fluoride use among pediatric patients; to analyze differences between ethnic and socioeconomic groups; and to describe the frequency of physician-parent discussions regarding water consumption. Convenience sample survey. An urban public health clinic. Parents attending a public health clinic. The primary outcome measure was the prevalence of tap, filtered, and bottled water use. The secondary outcome measures were supplemental fluoride use and the percentage of patients reporting discussions of water consumption with their physician. A total of 216 parents (80.5% Latino and 19.5% non-Latino) completed the survey. Of the parents, 30.1% never drank tap water and 41.2% never gave it to their children. Latino parents were less likely than non-Latino parents to drink tap water (odds ratio, 0.26; 95% confidence interval, 0.10-0.67) and less likely to give tap water to their children (odds ratio, 0.32; 95% confidence interval, 0.15-0.70). More Latinos believed that tap water would make them sick (odds ratio, 5.63; 95% confidence interval, 2.17-14.54). Approximately 40% of children who never drank tap water were not receiving fluoride supplements. Of the lowest-income families (

Mercury cycling in agricultural and managed wetlands, Yolo Bypass, California: Spatial and seasonal variations in water quality

USGS Publications Warehouse

Alpers, Charles N.; Fleck, Jacob A.; Marvin-DiPasquale, Mark C.; Stricker, Craig A.; Stephenson, Mark; Taylor, Howard E.

2014-01-01

The seasonal and spatial variability of water quality, including mercury species, was evaluated in agricultural and managed, non-agricultural wetlands in the Yolo Bypass Wildlife Area, an area managed for multiple beneficial uses including bird habitat and rice farming. The study was conducted during an 11-month period (June 2007 to April 2008) that included a summer growing season and flooded conditions during winter. Methylmercury (MeHg) concentrations in surface water varied over a wide range (0.1 to 37 ng L−1 unfiltered; 0.04 to 7.3 ng L−1 filtered). Maximum MeHg values are among the highest ever recorded in wetlands. Highest MeHg concentrations in unfiltered surface water were observed in drainage from wild rice fields during harvest (September 2007), and in white rice fields with decomposing rice straw during regional flooding (February 2008). The ratio of MeHg to total mercury (MeHg/THg) increased about 20-fold in both unfiltered and filtered water during the growing season (June to August 2007) in the white and wild rice fields, and about 5-fold in fallow fields (July to August 2007), while there was little to no change in MeHg/THg in the permanent wetland. Sulfate-bearing fertilizer had no effect on Hg(II) methylation, as sulfate-reducing bacteria were not sulfate limited in these agricultural wetlands. Concentrations of MeHg in filtered and unfiltered water correlated with filtered Fe, filtered Mn, DOC, and two indicators of sulfate reduction: the SO4 2 −/Cl− ratio, and δ34S in aqueous sulfate. These relationships suggest that microbial reduction of SO4 2−, Fe(III), and possibly Mn(IV) may contribute to net Hg(II)-methylation in this setting.

Progress in vacuum susceptibilities and their applications to the chiral phase transition of QCD

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cui, Zhu-Fang, E-mail: phycui@nju.edu.cn; State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, CAS, Beijing, 100190; Hou, Feng-Yao

2015-07-15

The QCD vacuum condensates and various vacuum susceptibilities are all important parameters which characterize the nonperturbative properties of the QCD vacuum. In the QCD sum rules external field formula, various QCD vacuum susceptibilities play important roles in determining the properties of hadrons. In this paper, we review the recent progress in studies of vacuum susceptibilities together with their applications to the chiral phase transition of QCD. The results of the tensor, the vector, the axial–vector, the scalar, and the pseudo-scalar vacuum susceptibilities are shown in detail in the framework of Dyson–Schwinger equations.