Miniature Spatial Heterodyne Raman Spectrometer with a Cell Phone Camera Detector.

PubMed

Barnett, Patrick D; Angel, S Michael

2017-05-01

A spatial heterodyne Raman spectrometer (SHRS) with millimeter-sized optics has been coupled with a standard cell phone camera as a detector for Raman measurements. The SHRS is a dispersive-based interferometer with no moving parts and the design is amenable to miniaturization while maintaining high resolution and large spectral range. In this paper, a SHRS with 2.5 mm diffraction gratings has been developed with 17.5 cm -1 theoretical spectral resolution. The footprint of the SHRS is orders of magnitude smaller than the footprint of charge-coupled device (CCD) detectors typically employed in Raman spectrometers, thus smaller detectors are being explored to shrink the entire spectrometer package. This paper describes the performance of a SHRS with 2.5 mm wide diffraction gratings and a cell phone camera detector, using only the cell phone's built-in optics to couple the output of the SHRS to the sensor. Raman spectra of a variety of samples measured with the cell phone are compared to measurements made using the same miniature SHRS with high-quality imaging optics and a high-quality, scientific-grade, thermoelectrically cooled CCD.

Night Sky Weather Monitoring System Using Fish-Eye CCD

NASA Astrophysics Data System (ADS)

Tomida, Takayuki; Saito, Yasunori; Nakamura, Ryo; Yamazaki, Katsuya

Telescope Array (TA) is international joint experiment observing ultra-high energy cosmic rays. TA employs fluorescence detection technique to observe cosmic rays. In this technique, tho existence of cloud significantly affects quality of data. Therefore, cloud monitoring provides important information. We are developing two new methods for evaluating night sky weather with pictures taken by charge-coupled device (CCD) camera. One is evaluating the amount of cloud with pixels brightness. The other is counting the number of stars with contour detection technique. The results of these methods show clear correlation, and we concluded both the analyses are reasonable methods for weather monitoring. We discuss reliability of the star counting method.

A compact CCD-monitored atomic force microscope with optical vision and improved performances.

PubMed

Mingyue, Liu; Haijun, Zhang; Dongxian, Zhang

2013-09-01

A novel CCD-monitored atomic force microscope (AFM) with optical vision and improved performances has been developed. Compact optical paths are specifically devised for both tip-sample microscopic monitoring and cantilever's deflection detecting with minimized volume and optimal light-amplifying ratio. The ingeniously designed AFM probe with such optical paths enables quick and safe tip-sample approaching, convenient and effective tip-sample positioning, and high quality image scanning. An image stitching method is also developed to build a wider-range AFM image under monitoring. Experiments show that this AFM system can offer real-time optical vision for tip-sample monitoring with wide visual field and/or high lateral optical resolution by simply switching the objective; meanwhile, it has the elegant performances of nanometer resolution, high stability, and high scan speed. Furthermore, it is capable of conducting wider-range image measurement while keeping nanometer resolution. Copyright © 2013 Wiley Periodicals, Inc.

Electronic cameras for low-light microscopy.

PubMed

Rasnik, Ivan; French, Todd; Jacobson, Ken; Berland, Keith

2013-01-01

This chapter introduces to electronic cameras, discusses the various parameters considered for evaluating their performance, and describes some of the key features of different camera formats. The chapter also presents the basic understanding of functioning of the electronic cameras and how these properties can be exploited to optimize image quality under low-light conditions. Although there are many types of cameras available for microscopy, the most reliable type is the charge-coupled device (CCD) camera, which remains preferred for high-performance systems. If time resolution and frame rate are of no concern, slow-scan CCDs certainly offer the best available performance, both in terms of the signal-to-noise ratio and their spatial resolution. Slow-scan cameras are thus the first choice for experiments using fixed specimens such as measurements using immune fluorescence and fluorescence in situ hybridization. However, if video rate imaging is required, one need not evaluate slow-scan CCD cameras. A very basic video CCD may suffice if samples are heavily labeled or are not perturbed by high intensity illumination. When video rate imaging is required for very dim specimens, the electron multiplying CCD camera is probably the most appropriate at this technological stage. Intensified CCDs provide a unique tool for applications in which high-speed gating is required. The variable integration time video cameras are very attractive options if one needs to acquire images at video rate acquisition, as well as with longer integration times for less bright samples. This flexibility can facilitate many diverse applications with highly varied light levels. Copyright © 2007 Elsevier Inc. All rights reserved.

CTK: A new CCD Camera at the University Observatory Jena

NASA Astrophysics Data System (ADS)

Mugrauer, M.

2009-05-01

The Cassegrain-Teleskop-Kamera (CTK) is a new CCD imager which is operated at the University Observatory Jena since begin of 2006. This article describes the main characteristics of the new camera. The properties of the CCD detector, the CTK image quality, as well as its detection limits for all filters are presented. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University.

CTK-II & RTK: The CCD-cameras operated at the auxiliary telescopes of the University Observatory Jena

NASA Astrophysics Data System (ADS)

Mugrauer, M.

2016-03-01

The Cassegrain-Teleskop-Kamera (CTK-II) and the Refraktor-Teleskop-Kamera (RTK) are two CCD-imagers which are operated at the 25 cm Cassegrain and 20 cm refractor auxiliary telescopes of the University Observatory Jena. This article describes the main characteristics of these instruments. The properties of the CCD-detectors, the astrometry, the image quality, and the detection limits of both CCD-cameras, as well as some results of ongoing observing projects, carried out with these instruments, are presented. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University.

The timing of events surrounding the Eocene-Oligocene boundary - Results from ODP Leg 199

NASA Astrophysics Data System (ADS)

Pälike, H.; Wilson, P. A.; Coxall, H.; Backman, J.

2003-04-01

The Eocene/Oligocene (E/O) boundary represents an extreme and rapid climatic transition from the ``greenhouse'' world of the Cretaceous and early Paleogene into the late Paleogene-Neogene ``ice-house''. It is marked by a large and global deepening in the calcite compensation depth (CCD), as well as pronounced changes in the isotopic composition of carbon and oxygen in seawater, recorded in biogenic calcium carbonate. A good understanding is still lacking as to why climatic, palaeoceanographic and marine biological productivity changes occurred within a few tens of thousands of years, and what change in boundary conditions triggered a non-linear response of the climate system. Detailed palaeoceanographic records surrounding the E/O have been rare because of the lack of well-dated, expanded deep-sea sedimentary sections containing well-preserved calcareous microfossils. Ocean Drilling Program Leg 199 recently recovered an extensive set of high-quality sediment cores across the E/O that span a latitudinal and depth transect in the central Pacific Ocean. We present new high-resolution records of bulk %CaCO3, δ18O and δ13C for a set of sites that form a depth transect, clearly delineating the relative depth with respect to the CCD during the transition. Our data show that a two-stepped deepening of the CCD coincides with a remarkably similar and simultaneous evolution of bulk δ18O values. We can demonstrate the imprint of climatic cycles around the E/O boundary, and very high-quality bio- and paleomagnetic datum points allow us to link these to Earth's orbital variations. Shipboard measurements of sediment properties and down-hole log measurements also display an imprint of climatic cycles, and allow us to obtain an astronomically calibrated time scale across the E/O. Our results put tighter constraints on the timing of the evolution of the CCD, mass accumulation rates, and biological productivity across the E/O, which display a distinct two-step shift in the most expanded section at the shallowest end of the transect (Site 1218). The initial deepening of the CCD occurred in less than 50 thousand years, and we observe a change in the nature and amplitude of climatic cycles that are recorded in sediments from Leg 199 across the E/O.

Agricultural produce grading and sorting system using color CCD and new color identification algorithm

NASA Astrophysics Data System (ADS)

Wang, Dongsheng; Zou, Jizuo; Yang, Yunping; Dong, Jianhua; Zhang, Yuanxiang

1996-10-01

A high-speed automatic agricultural produce grading and sorting system using color CCD and new color identification algorithm has been developed. In a typical application, the system can sort almonds into tow output grades according to their color. Almonds ar rich in 18 kinds of amino acids and 13 kinds of micro minerals and vitamins and can be made into almond drink. In order to ensure the drink quality, almonds must be sorted carefully before being made into a drink. Using this system, almonds can be sorted into two grades: up to grade and below grade almonds or foreign materials. A color CCD inspects the almonds passing on a conveyor of rotating rollers, a color identification algorithm grades almonds and distinguishes foreign materials from almonds. Employing an elaborately designed mechanism, the below grade almonds and foreign materials can be removed effectively from the raw almonds. This system can be easily adapted for inspecting and sorting other kinds of agricultural produce such as peanuts, beans tomatoes and so on.

Optimized algorithm for the spatial nonuniformity correction of an imaging system based on a charge-coupled device color camera.

PubMed

de Lasarte, Marta; Pujol, Jaume; Arjona, Montserrat; Vilaseca, Meritxell

2007-01-10

We present an optimized linear algorithm for the spatial nonuniformity correction of a CCD color camera's imaging system and the experimental methodology developed for its implementation. We assess the influence of the algorithm's variables on the quality of the correction, that is, the dark image, the base correction image, and the reference level, and the range of application of the correction using a uniform radiance field provided by an integrator cube. The best spatial nonuniformity correction is achieved by having a nonzero dark image, by using an image with a mean digital level placed in the linear response range of the camera as the base correction image and taking the mean digital level of the image as the reference digital level. The response of the CCD color camera's imaging system to the uniform radiance field shows a high level of spatial uniformity after the optimized algorithm has been applied, which also allows us to achieve a high-quality spatial nonuniformity correction of captured images under different exposure conditions.

Defect inspection in hot slab surface: multi-source CCD imaging based fuzzy-rough sets method

NASA Astrophysics Data System (ADS)

Zhao, Liming; Zhang, Yi; Xu, Xiaodong; Xiao, Hong; Huang, Chao

2016-09-01

To provide an accurate surface defects inspection method and make the automation of robust image region of interests(ROI) delineation strategy a reality in production line, a multi-source CCD imaging based fuzzy-rough sets method is proposed for hot slab surface quality assessment. The applicability of the presented method and the devised system are mainly tied to the surface quality inspection for strip, billet and slab surface etcetera. In this work we take into account the complementary advantages in two common machine vision (MV) systems(line array CCD traditional scanning imaging (LS-imaging) and area array CCD laser three-dimensional (3D) scanning imaging (AL-imaging)), and through establishing the model of fuzzy-rough sets in the detection system the seeds for relative fuzzy connectedness(RFC) delineation for ROI can placed adaptively, which introduces the upper and lower approximation sets for RIO definition, and by which the boundary region can be delineated by RFC region competitive classification mechanism. For the first time, a Multi-source CCD imaging based fuzzy-rough sets strategy is attempted for CC-slab surface defects inspection that allows an automatic way of AI algorithms and powerful ROI delineation strategies to be applied to the MV inspection field.

Online detecting system of roller wear based on laser-linear array CCD technology

NASA Astrophysics Data System (ADS)

Guo, Yuan

2010-10-01

Roller is an important metallurgy tool in the rolling mill. And the surface of a roller affects the quantity of the rolling product directly. After using a period of time, roller must be repaired or replaced. Examining the profile of a working roller between the intervals of rolling is called online detecting for roller wear. The study of online detecting roller wear is very important for selecting the grinding time in reason, reducing the exchanging times of rollers, improving the quality of the product and realizing online grinding rollers. By applying the laser-linear array CCD detective technology, a method for online non-touch detecting roller wear was brought forward. The principle, composition and the operation process of the linear array CCD detecting system were expatiated. And an error compensation algorithm is exactly calculated to offset the shift of the roller axis in this measurement system. So the stability and the accuracy were improved remarkably. The experiment proves that the accuracy of the detecting system reaches to the demand of practical production process. It can provide a new method of high speed and high accuracy online detecting for roller wear.

Radioactive Quality Evaluation and Cross Validation of Data from the HJ-1A/B Satellites' CCD Sensors

PubMed Central

Zhang, Xin; Zhao, Xiang; Liu, Guodong; Kang, Qian; Wu, Donghai

2013-01-01

Data from multiple sensors are frequently used in Earth science to gain a more complete understanding of spatial information changes. Higher quality and mutual consistency are prerequisites when multiple sensors are jointly used. The HJ-1A/B satellites successfully launched on 6 September 2008. There are four charge-coupled device (CCD) sensors with uniform spatial resolutions and spectral range onboard the HJ-A/B satellites. Whether these data are keeping consistency is a major issue before they are used. This research aims to evaluate the data consistency and radioactive quality from the four CCDs. First, images of urban, desert, lake and ocean are chosen as the objects of evaluation. Second, objective evaluation variables, such as mean, variance and angular second moment, are used to identify image performance. Finally, a cross validation method are used to ensure the correlation of the data from the four HJ-1A/B CCDs and that which is gathered from the moderate resolution imaging spectro-radiometer (MODIS). The results show that the image quality of HJ-1A/B CCDs is stable, and the digital number distribution of CCD data is relatively low. In cross validation with MODIS, the root mean square errors of bands 1, 2 and 3 range from 0.055 to 0.065, and for band 4 it is 0.101. The data from HJ-1A/B CCD have better consistency. PMID:23881127

Radioactive quality evaluation and cross validation of data from the HJ-1A/B satellites' CCD sensors.

PubMed

Zhang, Xin; Zhao, Xiang; Liu, Guodong; Kang, Qian; Wu, Donghai

2013-07-05

Data from multiple sensors are frequently used in Earth science to gain a more complete understanding of spatial information changes. Higher quality and mutual consistency are prerequisites when multiple sensors are jointly used. The HJ-1A/B satellites successfully launched on 6 September 2008. There are four charge-coupled device (CCD) sensors with uniform spatial resolutions and spectral range onboard the HJ-A/B satellites. Whether these data are keeping consistency is a major issue before they are used. This research aims to evaluate the data consistency and radioactive quality from the four CCDs. First, images of urban, desert, lake and ocean are chosen as the objects of evaluation. Second, objective evaluation variables, such as mean, variance and angular second moment, are used to identify image performance. Finally, a cross validation method are used to ensure the correlation of the data from the four HJ-1A/B CCDs and that which is gathered from the moderate resolution imaging spectro-radiometer (MODIS). The results show that the image quality of HJ-1A/B CCDs is stable, and the digital number distribution of CCD data is relatively low. In cross validation with MODIS, the root mean square errors of bands 1, 2 and 3 range from 0.055 to 0.065, and for band 4 it is 0.101. The data from HJ-1A/B CCD have better consistency.

High-frame rate multiport CCD imager and camera

NASA Astrophysics Data System (ADS)

Levine, Peter A.; Patterson, David R.; Esposito, Benjamin J.; Tower, John R.; Lawler, William B.

1993-01-01

A high frame rate visible CCD camera capable of operation up to 200 frames per second is described. The camera produces a 256 X 256 pixel image by using one quadrant of a 512 X 512 16-port, back illuminated CCD imager. Four contiguous outputs are digitally reformatted into a correct, 256 X 256 image. This paper details the architecture and timing used for the CCD drive circuits, analog processing, and the digital reformatter.

High-resolution CCD imaging alternatives

NASA Astrophysics Data System (ADS)

Brown, D. L.; Acker, D. E.

1992-08-01

High resolution CCD color cameras have recently stimulated the interest of a large number of potential end-users for a wide range of practical applications. Real-time High Definition Television (HDTV) systems are now being used or considered for use in applications ranging from entertainment program origination through digital image storage to medical and scientific research. HDTV generation of electronic images offers significant cost and time-saving advantages over the use of film in such applications. Further in still image systems electronic image capture is faster and more efficient than conventional image scanners. The CCD still camera can capture 3-dimensional objects into the computing environment directly without having to shoot a picture on film develop it and then scan the image into a computer. 2. EXTENDING CCD TECHNOLOGY BEYOND BROADCAST Most standard production CCD sensor chips are made for broadcast-compatible systems. One popular CCD and the basis for this discussion offers arrays of roughly 750 x 580 picture elements (pixels) or a total array of approximately 435 pixels (see Fig. 1). FOR. A has developed a technique to increase the number of available pixels for a given image compared to that produced by the standard CCD itself. Using an inter-lined CCD with an overall spatial structure several times larger than the photo-sensitive sensor areas each of the CCD sensors is shifted in two dimensions in order to fill in spatial gaps between adjacent sensors.

Using a delta-doped CCD to determine the energy of a low-energy particle

NASA Technical Reports Server (NTRS)

Nikzad, Shouleh (Inventor); Croley, Donald R. (Inventor); Murphy, Gerald B. (Inventor)

2001-01-01

The back surface of a thinned charged-coupled device (CCD) is treated to eliminate the backside potential well that appears in a conventional thinned CCD during backside illumination. The backside of the CCD includes a delta layer of high-concentration dopant confined to less than one monolayer of the crystal semiconductor. The thinned, delta-doped CCD is used to determine the energy of a very low-energy particle that penetrates less than 1.0 nm into the CCD, such as a proton having energy less than 10 keV.

Delta-doped CCD's as low-energy particle detectors and imagers

NASA Technical Reports Server (NTRS)

Nikzad, Shouleh (Inventor); Hoenk, Michael E. (Inventor); Hecht, Michael H. (Inventor)

2002-01-01

The back surface of a thinned charged-coupled device (CCD) is treated to eliminate the backside potential well that appears in a conventional thinned CCD during backside illumination. The backside of the CCD includes a delta layer of high-concentration dopant confined to less than one monolayer of the crystal semiconductor. The thinned, delta-doped CCD is used to detect very low-energy particles that penetrate less than 1.0 nm into the CCD, including electrons having energies less than 1000 eV and protons having energies less than 10 keV.

Update on the Status of the Space Telescope Imaging Spectrograph onboard the Hubble Space Telescope

NASA Astrophysics Data System (ADS)

Hernandez, Svea; Aloisi, A.; Bostroem, K. A.; Cox, C.; Debes, J. H.; DiFelice, A.; Roman-Duval, J.; Hodge, P.; Holland, S.; Lindsay, K.; Lockwood, S. A.; Mason, E.; Oliveira, C. M.; Penton, S. V.; Proffitt, C. R.; Sonnentrucker, P.; Taylor, J. M.; Wheeler, T.

2013-06-01

The Space Telescope Imaging Spectrograph (STIS) has been on orbit for approximately 16 years as one of the 2nd generation instruments on the Hubble Space Telescope (HST). Its operations were interrupted by an electronics failure in 2004, but STIS was successfully repaired in May 2009 during Service Mission 4 (SM4) allowing it to resume science observations. The Instrument team continues to monitor its performance and work towards improving the quality of its products. Here we present updated information on the status of the FUV and NUV MAMA and the CCD detectors onboard STIS and describe recent changes to the STIS calibration pipeline. We also discuss the status of efforts to apply a pixel-based correction for charge transfer inefficiency (CTI) effects to STIS CCD data. These techniques show promise for ameliorating the effects of ongoing radiation damage on the quality of STIS CCD data.

Choice and maintenance of equipment for electron crystallography.

PubMed

Mills, Deryck J; Vonck, Janet

2013-01-01

The choice of equipment for an electron crystallography laboratory will ultimately be determined by the available budget; nevertheless, the ideal lab will have two electron microscopes: a dedicated 300 kV cryo-EM with a field emission gun and a smaller LaB(6) machine for screening. The high-end machine should be equipped with photographic film or a very large CCD or CMOS camera for 2D crystal data collection; the screening microscope needs a mid-size CCD for rapid evaluation of crystal samples. The microscope room installations should provide adequate space and a special environment that puts no restrictions on the collection of high-resolution data. Equipment for specimen preparation includes a carbon coater, glow discharge unit, light microscope, plunge freezer, and liquid nitrogen containers and storage dewars. When photographic film is to be used, additional requirements are a film desiccator, dark room, optical diffractometer, and a film scanner. Having the electron microscopes and ancillary equipment well maintained and always in optimum condition facilitates the production of high-quality data.

Development of a Portable 3CCD Camera System for Multispectral Imaging of Biological Samples

PubMed Central

Lee, Hoyoung; Park, Soo Hyun; Noh, Sang Ha; Lim, Jongguk; Kim, Moon S.

2014-01-01

Recent studies have suggested the need for imaging devices capable of multispectral imaging beyond the visible region, to allow for quality and safety evaluations of agricultural commodities. Conventional multispectral imaging devices lack flexibility in spectral waveband selectivity for such applications. In this paper, a recently developed portable 3CCD camera with significant improvements over existing imaging devices is presented. A beam-splitter prism assembly for 3CCD was designed to accommodate three interference filters that can be easily changed for application-specific multispectral waveband selection in the 400 to 1000 nm region. We also designed and integrated electronic components on printed circuit boards with firmware programming, enabling parallel processing, synchronization, and independent control of the three CCD sensors, to ensure the transfer of data without significant delay or data loss due to buffering. The system can stream 30 frames (3-waveband images in each frame) per second. The potential utility of the 3CCD camera system was demonstrated in the laboratory for detecting defect spots on apples. PMID:25350510

Advances in CCD detector technology for x-ray diffraction applications

NASA Astrophysics Data System (ADS)

Thorson, Timothy A.; Durst, Roger D.; Frankel, Dan; Bordwell, Rex L.; Camara, Jose R.; Leon-Guerrero, Edward; Onishi, Steven K.; Pang, Francis; Vu, Paul; Westbrook, Edwin M.

2004-01-01

Phosphor-coupled CCDs are established as one of the most successful technologies for x-ray diffraction. This application demands that the CCD simultaneously achieve both the highest possible sensitivity and high readout speeds. Recently, wafer-scale, back illuminated devices have become available which offer significantly higher quantum efficiency than conventional devices (the Fairchild Imaging CCD 486 BI). However, since back thinning significantly changes the electrical properties of the CCD the high speed operation of wafer-scale, back-illuminated devices is not well understood. Here we describe the operating characteristics (including noise, linearity, full well capacity and CTE) of the back-illuminated CCD 486 at readout speeds up to 4 MHz.

Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buts, Lieven; De Jonge, Natalie; Loris, Remy, E-mail: reloris@vub.ac.be

2005-10-01

The CcdA C-terminal domain was crystallized in complex with CcdB in two crystal forms that diffract to beyond 2.0 Å resolution. CcdA and CcdB are the antidote and toxin of the ccd addiction module of Escherichia coli plasmid F. The CcdA C-terminal domain (CcdA{sub C36}; 36 amino acids) was crystallized in complex with CcdB (dimer of 2 × 101 amino acids) in three different crystal forms, two of which diffract to high resolution. Form II belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 37.6, b = 60.5, c = 83.8 Å and diffracts to 1.8more » Å resolution. Form III belongs to space group P2{sub 1}, with unit-cell parameters a = 41.0, b = 37.9, c = 69.6 Å, β = 96.9°, and diffracts to 1.9 Å resolution.« less

Fully depleted back illuminated CCD

DOEpatents

Holland, Stephen Edward

2001-01-01

A backside illuminated charge coupled device (CCD) is formed of a relatively thick high resistivity photon sensitive silicon substrate, with frontside electronic circuitry, and an optically transparent backside ohmic contact for applying a backside voltage which is at least sufficient to substantially fully deplete the substrate. A greater bias voltage which overdepletes the substrate may also be applied. One way of applying the bias voltage to the substrate is by physically connecting the voltage source to the ohmic contact. An alternate way of applying the bias voltage to the substrate is to physically connect the voltage source to the frontside of the substrate, at a point outside the depletion region. Thus both frontside and backside contacts can be used for backside biasing to fully deplete the substrate. Also, high resistivity gaps around the CCD channels and electrically floating channel stop regions can be provided in the CCD array around the CCD channels. The CCD array forms an imaging sensor useful in astronomy.

High-Voltage Clock Driver for Photon-Counting CCD Characterization

NASA Technical Reports Server (NTRS)

Baker, Robert

2013-01-01

A document discusses the CCD97 from e2v technologies as it is being evaluated at Goddard Space Flight Center's Detector Characterization Laboratory (DCL) for possible use in ultra-low background noise space astronomy applications, such as Terrestrial Planet Finder Coronagraph (TPF-C). The CCD97 includes a photoncounting mode where the equivalent output noise is less than one electron. Use of this mode requires a clock signal at a voltage level greater than the level achievable by the existing CCD (charge-coupled-device) electronics. A high-voltage waveform generator has been developed in code 660/601 to support the CCD97 evaluation. The unit generates required clock waveforms at voltage levels from -20 to +50 V. It deals with standard and arbitrary waveforms and supports pixel rates from 50 to 500 kHz. The system is designed to interface with existing Leach CCD electronics.

The design and development of low- and high-voltage ASICs for space-borne CCD cameras

NASA Astrophysics Data System (ADS)

Waltham, N.; Morrissey, Q.; Clapp, M.; Bell, S.; Jones, L.; Torbet, M.

2017-12-01

The CCD remains the pre-eminent visible and UV wavelength image sensor in space science, Earth and planetary remote sensing. However, the design of space-qualified CCD readout electronics is a significant challenge with requirements for low-volume, low-mass, low-power, high-reliability and tolerance to space radiation. Space-qualified components are frequently unavailable and up-screened commercial components seldom meet project or international space agency requirements. In this paper, we describe an alternative approach of designing and space-qualifying a series of low- and high-voltage mixed-signal application-specific integrated circuits (ASICs), the ongoing development of two low-voltage ASICs with successful flight heritage, and two new high-voltage designs. A challenging sub-system of any CCD camera is the video processing and digitisation electronics. We describe recent developments to improve performance and tolerance to radiation-induced single event latchup of a CCD video processing ASIC originally developed for NASA's Solar Terrestrial Relations Observatory and Solar Dynamics Observatory. We also describe a programme to develop two high-voltage ASICs to address the challenges presented with generating a CCD's bias voltages and drive clocks. A 0.35 μm, 50 V tolerant, CMOS process has been used to combine standard low-voltage 3.3 V transistors with high-voltage 50 V diffused MOSFET transistors that enable output buffers to drive CCD bias drains, gates and clock electrodes directly. We describe a CCD bias voltage generator ASIC that provides 24 independent and programmable 0-32 V outputs. Each channel incorporates a 10-bit digital-to-analogue converter, provides current drive of up to 20 mA into loads of 10 μF, and includes current-limiting and short-circuit protection. An on-chip telemetry system with a 12-bit analogue-to-digital converter enables the outputs and multiple off-chip camera voltages to be monitored. The ASIC can drive one or more CCDs and replaces the many discrete components required in current cameras. We also describe a CCD clock driver ASIC that provides six independent and programmable drivers with high-current capacity. The device enables various CCD clock parameters to be programmed independently, for example the clock-low and clock-high voltage levels, and the clock-rise and clock-fall times, allowing configuration for serial clock frequencies in the range 0.1-2 MHz and image clock frequencies in the range 10-100 kHz. Finally, we demonstrate the impact and importance of this technology for the development of compact, high-performance and low-power integrated focal plane electronics.

A Design and Development of Multi-Purpose CCD Camera System with Thermoelectric Cooling: Software

NASA Astrophysics Data System (ADS)

Oh, S. H.; Kang, Y. W.; Byun, Y. I.

2007-12-01

We present a software which we developed for the multi-purpose CCD camera. This software can be used on the all 3 types of CCD - KAF-0401E (768×512), KAF-1602E (15367times;1024), KAF-3200E (2184×1472) made in KODAK Co.. For the efficient CCD camera control, the software is operated with two independent processes of the CCD control program and the temperature/shutter operation program. This software is designed to fully automatic operation as well as manually operation under LINUX system, and is controled by LINUX user signal procedure. We plan to use this software for all sky survey system and also night sky monitoring and sky observation. As our results, the read-out time of each CCD are about 15sec, 64sec, 134sec for KAF-0401E, KAF-1602E, KAF-3200E., because these time are limited by the data transmission speed of parallel port. For larger format CCD, the data transmission is required more high speed. we are considering this control software to one using USB port for high speed data transmission.

Latest developments on the loop control system of AdOpt@TNG

NASA Astrophysics Data System (ADS)

Ghedina, Adriano; Gaessler, Wolfgang; Cecconi, Massimo; Ragazzoni, Roberto; Puglisi, Alfio T.; De Bonis, Fulvio

2004-10-01

The Adaptive Optics System of the Galileo Telescope (AdOpt@TNG) is the only adaptive optics system mounted on a telescope which uses a pyramid wavefront snesor and it has already shown on sky its potentiality. Recently AdOpt@TNG has undergone deep changes at the level of its higher orders control system. The CCD and the Real Time Computer (RTC) have been substituted as a whole. Instead of the VME based RTC, due to its frequent breakdowns, a dual pentium processor PC with Real-Time-Linux has been chosen. The WFS CCD, that feeds the images to the RTC, was changed to an off-the-shelf camera system from SciMeasure with an EEV39 80x80 pixels as detector. While the APD based Tip/Tilt loop has shown the quality on the sky at the TNG site and the ability of TNG to take advantage of this quality, up to the diffraction limit, the High-Order system has been fully re-developed and the performance of the closed loop is under evaluation to offer the system with the best performance to the astronomical community.

[Atmospheric correction of HJ-1 CCD data for water imagery based on dark object model].

PubMed

Zhou, Li-Guo; Ma, Wei-Chun; Gu, Wan-Hua; Huai, Hong-Yan

2011-08-01

The CCD multi-band data of HJ-1A has great potential in inland water quality monitoring, but the precision of atmospheric correction is a premise and necessary procedure for its application. In this paper, a method based on dark pixel for water-leaving radiance retrieving is proposed. Beside the Rayleigh scattering, the aerosol scattering is important to atmospheric correction, the water quality of inland lakes always are case II water and the value of water leaving radiance is not zero. So the synchronous MODIS shortwave infrared data was used to obtain the aerosol parameters, and in virtue of the characteristic that aerosol scattering is relative stabilized in 560 nm, the water-leaving radiance for each visible and near infrared band were retrieved and normalized, accordingly the remotely sensed reflectance of water was computed. The results show that the atmospheric correction method based on the imagery itself is more effective for the retrieval of water parameters for HJ-1A CCD data.

The research of digital circuit system for high accuracy CCD of portable Raman spectrometer

NASA Astrophysics Data System (ADS)

Yin, Yu; Cui, Yongsheng; Zhang, Xiuda; Yan, Huimin

2013-08-01

The Raman spectrum technology is widely used for it can identify various types of molecular structure and material. The portable Raman spectrometer has become a hot direction of the spectrometer development nowadays for its convenience in handheld operation and real-time detection which is superior to traditional Raman spectrometer with heavy weight and bulky size. But there is still a gap for its measurement sensitivity between portable and traditional devices. However, portable Raman Spectrometer with Shell-Isolated Nanoparticle-Enhanced Raman Spectroscopy (SHINERS) technology can enhance the Raman signal significantly by several orders of magnitude, giving consideration in both measurement sensitivity and mobility. This paper proposed a design and implementation of driver and digital circuit for high accuracy CCD sensor, which is core part of portable spectrometer. The main target of the whole design is to reduce the dark current generation rate and increase signal sensitivity during the long integration time, and in the weak signal environment. In this case, we use back-thinned CCD image sensor from Hamamatsu Corporation with high sensitivity, low noise and large dynamic range. In order to maximize this CCD sensor's performance and minimize the whole size of the device simultaneously to achieve the project indicators, we delicately designed a peripheral circuit for the CCD sensor. The design is mainly composed with multi-voltage circuit, sequential generation circuit, driving circuit and A/D transition parts. As the most important power supply circuit, the multi-voltage circuits with 12 independent voltages are designed with reference power supply IC and set to specified voltage value by the amplifier making up the low-pass filter, which allows the user to obtain a highly stable and accurate voltage with low noise. What's more, to make our design easy to debug, CPLD is selected to generate sequential signal. The A/D converter chip consists of a correlated double sampler; a digitally controlled variable gain amplifier and a 16-bit A/D converter which can help improve the data quality. And the acquired digital signals are transmitted into the computer via USB 2.0 data port. Our spectrometer with SHINERS technology can acquire the Raman spectrum signals efficiently in long time integration and weak signal environment, and the size of our system is well controlled for portable application.

Performance of the STIS CCD Dark Rate Temperature Correction

NASA Astrophysics Data System (ADS)

Branton, Doug; STScI STIS Team

2018-06-01

Since July 2001, the Space Telescope Imaging Spectrograph (STIS) onboard Hubble has operated on its Side-2 electronics due to a failure in the primary Side-1 electronics. While nearly identical, Side-2 lacks a functioning temperature sensor for the CCD, introducing a variability in the CCD operating temperature. Previous analysis utilized the CCD housing temperature telemetry to characterize the relationship between the housing temperature and the dark rate. It was found that a first-order 7%/°C uniform dark correction demonstrated a considerable improvement in the quality of dark subtraction on Side-2 era CCD data, and that value has been used on all Side-2 CCD darks since. In this report, we show how this temperature correction has performed historically. We compare the current 7%/°C value against the ideal first-order correction at a given time (which can vary between ~6%/°C and ~10%/°C) as well as against a more complex second-order correction that applies a unique slope to each pixel as a function of dark rate and time. At worst, the current correction has performed ~1% worse than the second-order correction. Additionally, we present initial evidence suggesting that the variability in pixel temperature-sensitivity is significant enough to warrant a temperature correction that considers pixels individually rather than correcting them uniformly.

Quasi-Speckle Measurements of Close Double Stars With a CCD Camera

NASA Astrophysics Data System (ADS)

Harshaw, Richard

2017-01-01

CCD measurements of visual double stars have been an active area of amateur observing for several years now. However, most CCD measurements rely on “lucky imaging” (selecting a very small percentage of the best frames of a larger frame set so as to get the best “frozen” atmosphere for the image), a technique that has limitations with regards to how close the stars can be and still be cleanly resolved in the lucky image. In this paper, the author reports how using deconvolution stars in the analysis of close double stars can greatly enhance the quality of the autocorellogram, leading to a more precise solution using speckle reduction software rather than lucky imaging.

Astronomical studies of the major planets, natural satellites and asteroids using the 2.24 m telescope

NASA Technical Reports Server (NTRS)

Jefferies, J. T.

1982-01-01

Directional features in the Jovian sodium torus, high quality CCD images of the major planets, methane bands in the spectrum of Triton, the central wavelength of the SO2 absorption band on Io, a component on the icy surfaces of the satellites of Uranus, fluctuation of Io's volcanic radiation, standard stars, and thermal radiation from the four brightest satellites of Uranus are discussed.

Automatic detection of recoil-proton tracks and background rejection criteria in liquid scintillator-micro-capillary-array fast neutron spectrometer

NASA Astrophysics Data System (ADS)

Mor, Ilan; Vartsky, David; Dangendorf, Volker; Tittelmeier, Kai.; Weierganz, Mathias; Goldberg, Mark Benjamin; Bar, Doron; Brandis, Michal

2018-06-01

We describe an analysis procedure for automatic unambiguous detection of fast-neutron-induced recoil proton tracks in a micro-capillary array filled with organic liquid scintillator. The detector is viewed by an intensified CCD camera. This imaging neutron detector possesses the capability to perform high position-resolution (few tens of μm), energy-dispersive transmission-imaging using ns-pulsed beams. However, when operated with CW or DC beams, it also features medium-quality spectroscopic capabilities for incident neutrons in the energy range 2-20 MeV. In addition to the recoil proton events which display a continuous extended track structure, the raw images exhibit complex ion-tracks from nuclear interactions of fast-neutrons in the scintillator, capillaries quartz-matrix and CCD. Moreover, as expected, one also observes a multitude of isolated scintillation spots of varying intensity (henceforth denoted "blobs") that originate from several different sources, such as: fragmented proton tracks, gamma-rays, heavy-ion reactions as well as events and noise that occur in the image-intensifier and CCD. In order to identify the continuous-track recoil proton events and distinguish them from all these background events, a rapid, computerized and automatic track-recognition-procedure was developed. Based on an appropriately weighted analysis of track parameters such as: length, width, area and overall light intensity, the method is capable of distinguishing a single continuous-track recoil proton from typically surrounding several thousands of background events that are found in each CCD frame.

Transmission electron microscope CCD camera

DOEpatents

Downing, Kenneth H.

1999-01-01

In order to improve the performance of a CCD camera on a high voltage electron microscope, an electron decelerator is inserted between the microscope column and the CCD. This arrangement optimizes the interaction of the electron beam with the scintillator of the CCD camera while retaining optimization of the microscope optics and of the interaction of the beam with the specimen. Changing the electron beam energy between the specimen and camera allows both to be optimized.

6 x 6-cm fully depleted pn-junction CCD for high-resolution spectroscopy in the 0.1- to 15-keV photon energy range

NASA Astrophysics Data System (ADS)

von Zanthier, Christoph; Holl, Peter; Kemmer, Josef; Lechner, Peter; Maier, B.; Soltau, Heike; Stoetter, R.; Braeuninger, Heinrich W.; Dennerl, Konrad; Haberl, Frank; Hartmann, R.; Hartner, Gisela D.; Hippmann, H.; Kastelic, E.; Kink, W.; Krause, N.; Meidinger, Norbert; Metzner, G.; Pfeffermann, Elmar; Popp, M.; Reppin, Claus; Stoetter, Diana; Strueder, Lothar; Truemper, Joachim; Weber, U.; Carathanassis, D.; Engelhard, S.; Gebhart, Th.; Hauff, D.; Lutz, G.; Richter, R. H.; Seitz, H.; Solc, P.; Bihler, Edgar; Boettcher, H.; Kendziorra, Eckhard; Kraemer, J.; Pflueger, Bernhard; Staubert, Ruediger

1998-04-01

The concept and performance of the fully depleted pn- junction CCD system, developed for the European XMM- and the German ABRIXAS-satellite missions for soft x-ray imaging and spectroscopy in the 0.1 keV to 15 keV photon range, is presented. The 58 mm X 60 mm large pn-CCD array uses pn- junctions for registers and for the backside instead of MOS registers. This concept naturally allows to fully deplete the detector volume to make it an efficient detector to photons with energies up to 15 keV. For high detection efficiency in the soft x-ray region down to 100 eV, an ultrathin pn-CCD backside deadlayer has been realized. Each pn-CCD-channel is equipped with an on-chip JFET amplifier which, in combination with the CAMEX-amplifier and multiplexing chip, facilitates parallel readout with a pixel read rate of 3 MHz and an electronic noise floor of ENC < e-. With the complete parallel readout, very fast pn-CCD readout modi can be implemented in the system which allow for high resolution photon spectroscopy of even the brightest x-ray sources in the sky.

Systems approach to the design of the CCD sensors and camera electronics for the AIA and HMI instruments on solar dynamics observatory

NASA Astrophysics Data System (ADS)

Waltham, N.; Beardsley, S.; Clapp, M.; Lang, J.; Jerram, P.; Pool, P.; Auker, G.; Morris, D.; Duncan, D.

2017-11-01

Solar Dynamics Observatory (SDO) is imaging the Sun in many wavelengths near simultaneously and with a resolution ten times higher than the average high-definition television. In this paper we describe our innovative systems approach to the design of the CCD cameras for two of SDO's remote sensing instruments, the Atmospheric Imaging Assembly (AIA) and the Helioseismic and Magnetic Imager (HMI). Both instruments share use of a custom-designed 16 million pixel science-grade CCD and common camera readout electronics. A prime requirement was for the CCD to operate with significantly lower drive voltages than before, motivated by our wish to simplify the design of the camera readout electronics. Here, the challenge lies in the design of circuitry to drive the CCD's highly capacitive electrodes and to digitize its analogue video output signal with low noise and to high precision. The challenge is greatly exacerbated when forced to work with only fully space-qualified, radiation-tolerant components. We describe our systems approach to the design of the AIA and HMI CCD and camera electronics, and the engineering solutions that enabled us to comply with both mission and instrument science requirements.

pnCCD for photon detection from near-infrared to X-rays

NASA Astrophysics Data System (ADS)

Meidinger, Norbert; Andritschke, Robert; Hartmann, Robert; Herrmann, Sven; Holl, Peter; Lutz, Gerhard; Strüder, Lothar

2006-09-01

A pnCCD is a special type of charge-coupled device developed for spectroscopy and imaging of X-rays with high time resolution and quantum efficiency. Its most famous application is the operation on the XMM-Newton satellite, an X-ray astronomy mission that was launched by the European space agency in 1999. The excellent performance of the focal plane camera has been maintained for more than 6 years in orbit. The energy resolution in particular has shown hardly any degradation since launch. In order to satisfy the requirements of future X-ray astronomy missions as well as those of ground-based experiments, a new type of pnCCD has been developed. This ‘frame-store pnCCD’ shows an enhanced performance compared to the XMM-Newton type of pnCCD. Now, more options in device design and operation are available to tailor the detector to its respective application. Part of this concept is a programmable analog signal processor, which has been developed for the readout of the CCD signals. The electronic noise of the new detector has a value of only 2 electrons equivalent noise charge (ENC), which is less than half of the figure achieved for the XMM-Newton-type pnCCD. The energy resolution for the Mn-Kα line at 5.9 keV is approximately 130 eV FWHM. We have close to 100% quantum efficiency for both low- and high-energy photon detection (e.g. the C-K line at 277 eV, and the Ge-Kα line at 10 keV, respectively). Very high frame rates of 1000 images/s have been achieved due to the ultra-fast readout accomplished by the parallel architecture of the pnCCD and the analog signal processor. Excellent spectroscopic performance is shown even at the relatively high operating temperature of -25 °C that can be achieved by a Peltier cooler. The applications of the low-noise and fast pnCCD detector are not limited to the detection of X-rays. With an anti-reflective coating deposited on the photon entrance window, we achieve high quantum efficiency also for near-infrared and optical photons. A novel type of pnCCD is in preparation, which allows single optical photon counting. This feature is accomplished by implementation of an avalanche-type amplifier in the pnCCD concept.

Coaxial fundus camera for opthalmology

NASA Astrophysics Data System (ADS)

de Matos, Luciana; Castro, Guilherme; Castro Neto, Jarbas C.

2015-09-01

A Fundus Camera for ophthalmology is a high definition device which needs to meet low light illumination of the human retina, high resolution in the retina and reflection free image1. Those constraints make its optical design very sophisticated, but the most difficult to comply with is the reflection free illumination and the final alignment due to the high number of non coaxial optical components in the system. Reflection of the illumination, both in the objective and at the cornea, mask image quality, and a poor alignment make the sophisticated optical design useless. In this work we developed a totally axial optical system for a non-midriatic Fundus Camera. The illumination is performed by a LED ring, coaxial with the optical system and composed of IR of visible LEDs. The illumination ring is projected by the objective lens in the cornea. The Objective, LED illuminator, CCD lens are coaxial making the final alignment easily to perform. The CCD + capture lens module is a CCTV camera with autofocus and Zoom built in, added to a 175 mm focal length doublet corrected for infinity, making the system easily operated and very compact.

Onboard TDI stage estimation and calibration using SNR analysis

NASA Astrophysics Data System (ADS)

Haghshenas, Javad

2017-09-01

Electro-Optical design of a push-broom space camera for a Low Earth Orbit (LEO) remote sensing satellite is performed based on the noise analysis of TDI sensors for very high GSDs and low light level missions. It is well demonstrated that the CCD TDI mode of operation provides increased photosensitivity relative to a linear CCD array, without the sacrifice of spatial resolution. However, for satellite imaging, in order to utilize the advantages which the TDI mode of operation offers, attention should be given to the parameters which affect the image quality of TDI sensors such as jitters, vibrations, noises and etc. A predefined TDI stages may not properly satisfy image quality requirement of the satellite camera. Furthermore, in order to use the whole dynamic range of the sensor, imager must be capable to set the TDI stages in every shots based on the affecting parameters. This paper deals with the optimal estimation and setting the stages based on tradeoffs among MTF, noises and SNR. On-board SNR estimation is simulated using the atmosphere analysis based on the MODTRAN algorithm in PcModWin software. According to the noises models, we have proposed a formulation to estimate TDI stages in such a way to satisfy the system SNR requirement. On the other hand, MTF requirement must be satisfy in the same manner. A proper combination of both parameters will guaranty the full dynamic range usage along with the high SNR and image quality.

Atomically Precise Surface Engineering for Producing Imagers

NASA Technical Reports Server (NTRS)

Nikzad, Shouleh (Inventor); Hoenk, Michael E. (Inventor); Greer, Frank (Inventor); Jones, Todd J. (Inventor)

2015-01-01

High-quality surface coatings, and techniques combining the atomic precision of molecular beam epitaxy and atomic layer deposition, to fabricate such high-quality surface coatings are provided. The coatings made in accordance with the techniques set forth by the invention are shown to be capable of forming silicon CCD detectors that demonstrate world record detector quantum efficiency (>50%) in the near and far ultraviolet (155 nm-300 nm). The surface engineering approaches used demonstrate the robustness of detector performance that is obtained by achieving atomic level precision at all steps in the coating fabrication process. As proof of concept, the characterization, materials, and exemplary devices produced are presented along with a comparison to other approaches.

Timing generator of scientific grade CCD camera and its implementation based on FPGA technology

NASA Astrophysics Data System (ADS)

Si, Guoliang; Li, Yunfei; Guo, Yongfei

2010-10-01

The Timing Generator's functions of Scientific Grade CCD Camera is briefly presented: it generates various kinds of impulse sequence for the TDI-CCD, video processor and imaging data output, acting as the synchronous coordinator for time in the CCD imaging unit. The IL-E2TDI-CCD sensor produced by DALSA Co.Ltd. use in the Scientific Grade CCD Camera. Driving schedules of IL-E2 TDI-CCD sensor has been examined in detail, the timing generator has been designed for Scientific Grade CCD Camera. FPGA is chosen as the hardware design platform, schedule generator is described with VHDL. The designed generator has been successfully fulfilled function simulation with EDA software and fitted into XC2VP20-FF1152 (a kind of FPGA products made by XILINX). The experiments indicate that the new method improves the integrated level of the system. The Scientific Grade CCD camera system's high reliability, stability and low power supply are achieved. At the same time, the period of design and experiment is sharply shorted.

Improvement in the light sensitivity of the ultrahigh-speed high-sensitivity CCD with a microlens array

NASA Astrophysics Data System (ADS)

Hayashida, T.,; Yonai, J.; Kitamura, K.; Arai, T.; Kurita, T.; Tanioka, K.; Maruyama, H.; Etoh, T. Goji; Kitagawa, S.; Hatade, K.; Yamaguchi, T.; Takeuchi, H.; Iida, K.

2008-02-01

We are advancing the development of ultrahigh-speed, high-sensitivity CCDs for broadcast use that are capable of capturing smooth slow-motion videos in vivid colors even where lighting is limited, such as at professional baseball games played at night. We have already developed a 300,000 pixel, ultrahigh-speed CCD, and a single CCD color camera that has been used for sports broadcasts and science programs using this CCD. However, there are cases where even higher sensitivity is required, such as when using a telephoto lens during a baseball broadcast or a high-magnification microscope during science programs. This paper provides a summary of our experimental development aimed at further increasing the sensitivity of CCDs using the light-collecting effects of a microlens array.

MTF measurement of LCDs by a linear CCD imager: I. Monochrome case

NASA Astrophysics Data System (ADS)

Kim, Tae-hee; Choe, O. S.; Lee, Yun Woo; Cho, Hyun-Mo; Lee, In Won

1997-11-01

We construct the modulation transfer function (MTF) measurement system of a LCD using a linear charge-coupled device (CCD) imager. The MTF used in optical system can not describe in the effect of both resolution and contrast on the image quality of display. Thus we present the new measurement method based on the transmission property of a LCD. While controlling contrast and brightness levels, the MTF is measured. From the result, we show that the method is useful for describing of the image quality. A ne measurement method and its condition are described. To demonstrate validity, the method is applied for comparison of the performance of two different LCDs.

Widefield Imaging: Selected Strategies for Processing Light-Contaminated Data

NASA Astrophysics Data System (ADS)

Cannistra, Stephen A.

The beauty of nebulae, galaxies, and star clusters takes on new meaning when portrayed in a widefield view, where familiar objects that are commonly seen in isolation are now shown in relation to one another. Through the use of high quality, short focal length optics combined with large, commercially available CCD chips, it is possible to capture broad regions of sky that an older generation of astrophotographers could only dream of.

A Photometric Observing Program at the VATT: Setting Up a Calibration Field

NASA Astrophysics Data System (ADS)

Davis Philip, A. G.; Boyle, R. P.; Janusz, R.

2009-05-01

Philip and Boyle have been making Strömgren and then Strömvil photometric observations of open and globular clusters at the Vatican Advanced Technology Telescope located on Mt. Graham in Arizona. Our aim is to obtain CCD photometric indices good to 0.01 magnitude. Indices of this quality can later be analyzed to yield estimates of temperature, luminosity and metallicity. But we have found that the CCD chip does not yield photometry of this quality without further corrections. Our most observed cluster is the open cluster, M 67. This cluster is also very well observed in the literature. We took the best published values and created a set of "standard" stars for our field. Taking our CCD results we could calculate deltas, as a function of position on the chip, which we then applied to all the CCD frames that we obtained. With this procedure we were able to obtain the precision of 0.01 magnitudes in all the fields that we observed. When we started we were able to use the "A" two-inch square Strömgren four-color set from KPNO. Later the Vatican Observatory bought a set of 3.48 inch square Strömgren filters, The Vatican Observatory had a set of circular Vilnius filters There was also an X filter. These eight filters made our Strömvil set.

The prevalence and geographic distribution of complex co-occurring disorders: a population study.

PubMed

Somers, J M; Moniruzzaman, A; Rezansoff, S N; Brink, J; Russolillo, A

2016-06-01

A subset of people with co-occurring substance use and mental disorders require coordinated support from health, social welfare and justice agencies to achieve diversion from homelessness, criminal recidivism and further health and social harms. Integrated models of care are typically concentrated in large urban centres. The present study aimed to empirically measure the prevalence and distribution of complex co-occurring disorders (CCD) in a large geographic region that includes urban as well as rural and remote settings. Linked data were examined in a population of roughly 3.7 million adults. Inclusion criteria for the CCD subpopulation were: physician diagnosed substance use and mental disorders; psychiatric hospitalisation; shelter assistance; and criminal convictions. Prevalence per 100 000 was calculated in 91 small areas representing urban, rural and remote settings. 2202 individuals met our inclusion criteria for CCD. Participants had high rates of hospitalisation (8.2 admissions), criminal convictions (8.6 sentences) and social assistance payments (over $36 000 CDN) in the past 5 years. There was wide variability in the geographic distribution of people with CCD, with high prevalence rates in rural and remote settings. People with CCD are not restricted to areas with large populations or to urban settings. The highest per capita rates of CCD were observed in relatively remote locations, where mental health and substance use services are typically in limited supply. Empirically supported interventions must be adapted to meet the needs of people living outside of urban settings with high rates of CCD.

Development and use of an L3CCD high-cadence imaging system for Optical Astronomy

NASA Astrophysics Data System (ADS)

Sheehan, Brendan J.; Butler, Raymond F.

2008-02-01

A high cadence imaging system, based on a Low Light Level CCD (L3CCD) camera, has been developed for photometric and polarimetric applications. The camera system is an iXon DV-887 from Andor Technology, which uses a CCD97 L3CCD detector from E2V technologies. This is a back illuminated device, giving it an extended blue response, and has an active area of 512×512 pixels. The camera system allows frame-rates ranging from 30 fps (full frame) to 425 fps (windowed & binned frame). We outline the system design, concentrating on the calibration and control of the L3CCD camera. The L3CCD detector can be either triggered directly by a GPS timeserver/frequency generator or be internally triggered. A central PC remotely controls the camera computer system and timeserver. The data is saved as standard `FITS' files. The large data loads associated with high frame rates, leads to issues with gathering and storing the data effectively. To overcome such problems, a specific data management approach is used, and a Python/PYRAF data reduction pipeline was written for the Linux environment. This uses calibration data collected either on-site, or from lab based measurements, and enables a fast and reliable method for reducing images. To date, the system has been used twice on the 1.5 m Cassini Telescope in Loiano (Italy) we present the reduction methods and observations made.

Soft x-ray imager (SXI) onboard the NeXT satellite

NASA Astrophysics Data System (ADS)

Tsuru, Takeshi Go; Takagi, Shin-Ichiro; Matsumoto, Hironori; Inui, Tatsuya; Ozawa, Midori; Koyama, Katsuji; Tsunemi, Hiroshi; Hayashida, Kiyoshi; Miyata, Emi; Ozawa, Hideki; Touhiguchi, Masakuni; Matsuura, Daisuke; Dotani, Tadayasu; Ozaki, Masanobu; Murakami, Hiroshi; Kohmura, Takayoshi; Kitamoto, Shunji; Awaki, Hisamitsu

2006-06-01

We give overview and the current status of the development of the Soft X-ray Imager (SXI) onboard the NeXT satellite. SXI is an X-ray CCD camera placed at the focal plane detector of the Soft X-ray Telescopes for Imaging (SXT-I) onboard NeXT. The pixel size and the format of the CCD is 24 x 24μm (IA) and 2048 x 2048 x 2 (IA+FS). Currently, we have been developing two types of CCD as candidates for SXI, in parallel. The one is front illumination type CCD with moderate thickness of the depletion layer (70 ~ 100μm) as a baseline plan. The other one is the goal plan, in which we develop back illumination type CCD with a thick depletion layer (200 ~ 300μm). For the baseline plan, we successfully developed the proto model 'CCD-NeXT1' with the pixel size of 12μm x 12μm and the CCD size of 24mm x 48mm. The depletion layer of the CCD has reached 75 ~ 85μm. The goal plan is realized by introduction of a new type of CCD 'P-channel CCD', which collects holes in stead of electrons in the common 'N-channel CCD'. By processing a test model of P-channel CCD we have confirmed high quantum efficiency above 10 keV with an equivalent depletion layer of 300μm. A back illumination type of P-channel CCD with a depletion layer of 200μm with aluminum coating for optical blocking has been also successfully developed. We have been also developing a thermo-electric cooler (TEC) with the function of the mechanically support of the CCD wafer without standoff insulators, for the purpose of the reduction of thermal input to the CCD through the standoff insulators. We have been considering the sensor housing and the onboard electronics for the CCD clocking, readout and digital processing of the frame date.

Experimental research on thermal conductive fillers for CCD module in space borne optical remote sensor

NASA Astrophysics Data System (ADS)

Zeng, Yi; Han, Xue-bing; Yang, Dong-shang; Gui, Li-jia; Zhao, Xiao-xiang; Si, Fu-qi

2016-03-01

A space-borne differential optical absorption spectrometer is a high precision aerospace optical remote sensor. It obtains the hyper-spectral，high spatial resolution radiation information by using the spectrometer with CCD（Charge Coupled Device）array detectors. Since a few CCDs are used as the key detector, the performance of the entire instrument is greatly affected by working condition of CCDs. The temperature of CCD modules has a great impact on the instrument measurement accuracy. It requires strict temperature control. The selection of the thermal conductive filler sticking CCD to the radiator is important in the CCD thermal design. Besides，due tothe complex and compact structure, it needs to take into account the anti-pollution of the optical system. Therefore, it puts forward high requirements on the selection of the conductive filler. In this paper, according to the structure characteristics of the CCD modules and the distribution of heat consumption, the thermal analysis tool I-DEAS/TMG is utilized to compute and simulate the temperature level of the CCD modules, while filling in thermal grease and thermal pad respectively. The temperature distribution of CCD heat dissipation in typical operating conditions is obtained. In addition, the heat balance test was carried out under the condition of two kinds of thermal conductive fillers. The thermal control of CCD was tested under various conditions, and the results were compared with the results of thermal analysis. The results show that there are some differences in thermal performance between the two kinds of thermal conductive fillers. Although they both can meet the thermal performance requirements of the instrument, either would be chosen taking account of other conditions and requirements such as anti-pollution and insulation. The content and results of this paper will be a good reference for the thermal design of the CCD in the aerospace optical payload.

The development of large-aperture test system of infrared camera and visible CCD camera

NASA Astrophysics Data System (ADS)

Li, Yingwen; Geng, Anbing; Wang, Bo; Wang, Haitao; Wu, Yanying

2015-10-01

Infrared camera and CCD camera dual-band imaging system is used in many equipment and application widely. If it is tested using the traditional infrared camera test system and visible CCD test system, 2 times of installation and alignment are needed in the test procedure. The large-aperture test system of infrared camera and visible CCD camera uses the common large-aperture reflection collimator, target wheel, frame-grabber, computer which reduces the cost and the time of installation and alignment. Multiple-frame averaging algorithm is used to reduce the influence of random noise. Athermal optical design is adopted to reduce the change of focal length location change of collimator when the environmental temperature is changing, and the image quality of the collimator of large field of view and test accuracy are also improved. Its performance is the same as that of the exotic congener and is much cheaper. It will have a good market.

Novel low-cost vision-sensing technology with controllable of exposal time for welding

NASA Astrophysics Data System (ADS)

Zhang, Wenzeng; Wang, Bin; Chen, Nian; Cao, Yipeng

2005-02-01

In the process of robot Welding, position of welding seam and welding pool shape is detected by CCD camera for quality control and seam tracking in real-time. It is difficult to always get a clear welding image in some welding methods, such as TIG welding. A novel idea that the exposal time of CCD camera is automatically controlled by arc voltage or arc luminance is proposed to get clear welding image. A set of special device and circuits are added to a common industrial CCD camera in order to flexibly control the CCD to start or close exposal by control of the internal clearing signal of the accumulated charge. Two special vision sensors according to the idea are developed. Their exposal grabbing can be triggered respectively by the arc voltage and the variety of the arc luminance. Two prototypes have been designed and manufactured. Experiments show that they can stably grab clear welding images at appointed moment, which is a basic for the feedback control of automatic welding.

Microtomography with photon counting detectors: improving the quality of tomographic reconstruction by voxel-space oversampling

NASA Astrophysics Data System (ADS)

Dudak, J.; Zemlicka, J.; Karch, J.; Hermanova, Z.; Kvacek, J.; Krejci, F.

2017-01-01

Photon counting detectors Timepix are known for their unique properties enabling X-ray imaging with extremely high contrast-to-noise ratio. Their applicability has been recently further improved since a dedicated technique for assembling large area Timepix detector arrays was introduced. Despite the fact that the sensitive area of Timepix detectors has been significantly increased, the pixel pitch is kept unchanged (55 microns). This value is much larger compared to widely used and popular X-ray imaging cameras utilizing scintillation crystals and CCD-based read-out. On the other hand, photon counting detectors provide steeper point-spread function. Therefore, with given effective pixel size of an acquired radiography, Timepix detectors provide higher spatial resolution than X-ray cameras with scintillation-based devices unless the image is affected by penumbral blur. In this paper we take an advance of steep PSF of photon counting detectors and test the possibility to improve the quality of computed tomography reconstruction using finer sampling of reconstructed voxel space. The achieved results are presented in comparison with data acquired under the same conditions using a commercially available state-of-the-art CCD X-ray camera.

Event-Driven X-Ray CCD Detectors for High Energy Astrophysics

NASA Technical Reports Server (NTRS)

Ricker, George R.

2004-01-01

A viewgraph presentation describing the Event-Driven X- Ray CCD (EDCCD) detector system for high energy astrophysics is presented. The topics include: 1) EDCCD: Description and Advantages; 2) Summary of Grant Activity Carried Out; and 3) EDCCD Test System.

High-speed imaging using 3CCD camera and multi-color LED flashes

NASA Astrophysics Data System (ADS)

Hijazi, Ala; Friedl, Alexander; Cierpka, Christian; Kähler, Christian; Madhavan, Vis

2017-11-01

This paper demonstrates the possibility of capturing full-resolution, high-speed image sequences using a regular 3CCD color camera in conjunction with high-power light emitting diodes of three different colors. This is achieved using a novel approach, referred to as spectral-shuttering, where a high-speed image sequence is captured using short duration light pulses of different colors that are sent consecutively in very close succession. The work presented in this paper demonstrates the feasibility of configuring a high-speed camera system using low cost and readily available off-the-shelf components. This camera can be used for recording six-frame sequences at frame rates up to 20 kHz or three-frame sequences at even higher frame rates. Both color crosstalk and spatial matching between the different channels of the camera are found to be within acceptable limits. A small amount of magnification difference between the different channels is found and a simple calibration procedure for correcting the images is introduced. The images captured using the approach described here are of good quality to be used for obtaining full-field quantitative information using techniques such as digital image correlation and particle image velocimetry. A sequence of six high-speed images of a bubble splash recorded at 400 Hz is presented as a demonstration.

Multipurpose Hyperspectral Imaging System

NASA Technical Reports Server (NTRS)

Mao, Chengye; Smith, David; Lanoue, Mark A.; Poole, Gavin H.; Heitschmidt, Jerry; Martinez, Luis; Windham, William A.; Lawrence, Kurt C.; Park, Bosoon

2005-01-01

A hyperspectral imaging system of high spectral and spatial resolution that incorporates several innovative features has been developed to incorporate a focal plane scanner (U.S. Patent 6,166,373). This feature enables the system to be used for both airborne/spaceborne and laboratory hyperspectral imaging with or without relative movement of the imaging system, and it can be used to scan a target of any size as long as the target can be imaged at the focal plane; for example, automated inspection of food items and identification of single-celled organisms. The spectral resolution of this system is greater than that of prior terrestrial multispectral imaging systems. Moreover, unlike prior high-spectral resolution airborne and spaceborne hyperspectral imaging systems, this system does not rely on relative movement of the target and the imaging system to sweep an imaging line across a scene. This compact system (see figure) consists of a front objective mounted at a translation stage with a motorized actuator, and a line-slit imaging spectrograph mounted within a rotary assembly with a rear adaptor to a charged-coupled-device (CCD) camera. Push-broom scanning is carried out by the motorized actuator which can be controlled either manually by an operator or automatically by a computer to drive the line-slit across an image at a focal plane of the front objective. To reduce the cost, the system has been designed to integrate as many as possible off-the-shelf components including the CCD camera and spectrograph. The system has achieved high spectral and spatial resolutions by using a high-quality CCD camera, spectrograph, and front objective lens. Fixtures for attachment of the system to a microscope (U.S. Patent 6,495,818 B1) make it possible to acquire multispectral images of single cells and other microscopic objects.

Clinical prototype of a plastic water-equivalent scintillating fiber dosimeter array for QA applications.

PubMed

Lacroix, Fréderic; Archambault, Louis; Gingras, Luc; Guillot, Mathieu; Beddar, A Sam; Beaulieu, Luc

2008-08-01

A clinical prototype of a scintillating fiber dosimeter array for quality assurance applications is presented. The array consists of a linear array of 29 plastic scintillation detectors embedded in a water-equivalent plastic sheet coupled to optical fibers used to guide optical photons to a charge coupled device (CCD) camera. The CCD is packaged in a light-tight, radiation-shielded housing designed for convenient transport. A custom designed connector is used to ensure reproducible mechanical positioning of the optical fibers relative to the CCD. Profile and depth dose characterization measurements are presented and show that the prototype provides excellent dose measurement reproducibility (+/-0.8%) in-field and good accuracy (+/-1.6% maximum deviation) relative to the dose measured with an IC10 ionization chamber.

Spectrum of Celiac disease in Paediatric population: Experience of Tertiary Care Center from Pakistan.

PubMed

Aziz, Danish Abdul; Kahlid, Misha; Memon, Fozia; Sadiq, Kamran

2017-01-01

To determine clinical features and relevant laboratory investigations of patient with celiac disease (CD) and comparing classical celiac disease (CCD) with Non-diarrheal celiac disease (NDCD). This is a five years retrospective study conducted at The Aga Khan University Hospital Karachi, Pakistan from January 2010 to December 2015, enrolling children from one year to 15 years of either gender diagnosed as celiac disease in accordance with revised ESPGHAN criteria. Biopsy samples with grade 2 or more on Modified Marsh Classification were considered as consistent with celiac disease. Celiac patients were categorized into Classical celiac disease (with Chronic Diarrhea) and non-diarrheal celiac disease (Atypical celiac) and their clinical features and relevant laboratory investigations were documented. Total 66 patients were selected with celiac disease according to inclusion criteria, 39 (59.09%) patients were labeled as CCD and 27 (40.91%) patients were labeled as NDCD. Marsh grading 3a and above were more marked in CCD as compared to NDCD. Mean titer for Tissue transglutaminase antibodies (TTG) were higher in CCD group in comparison to NDCD group. In CCD, the most common clinical presentations were abdominal distension whereas in NDCD, the most remarkable features were recurrent abdominal pain (62.9%). Frequency of failure to thrive is significantly high in CCD (82.05%) but patients merely with short stature were more common in NDCD (33.3%). Refractory anemia was present in 66.6% patients in NDCD group and 41.1% patients in CCD group. 74.3% patients in CCD group were vitamin D deficient whereas 85% patient had vitamin D deficiency in NDCD group (p= 0.03). NDCD is not uncommon in our population. Recurrent abdominal pain, failure to thrive or patients only with short stature and refractory anemia are prominent features in NCDC group whereas abdominal distension, failure to thrive and recurrent abdominal pain were noticeable features in CCD. High grade histopathology and raised antibodies titer is hallmark of CCD. Vitamin D deficiency is almost equally present in both groups.

Test of CCD Precision Limits for Differential Photometry

NASA Technical Reports Server (NTRS)

Borucki, W. J.; Dunham, E. W.; Wei, M. Z.; Robinson, L. B.; Ford, C. H.; Granados, A. F.

1995-01-01

Results of tests to demonstrate the very high differential-photometric stability of CCD light sensors are presented. The measurements reported here demonstrate that in a controlled laboratory environment, a front-illuminated CCD can provide differential-photometric measurements with reproducible precision approaching one part in 105. Practical limitations to the precision of differential-photometric measurements with CCDs and implications for spaceborne applications are discussed.

Multiple Sensor Camera for Enhanced Video Capturing

NASA Astrophysics Data System (ADS)

Nagahara, Hajime; Kanki, Yoshinori; Iwai, Yoshio; Yachida, Masahiko

A resolution of camera has been drastically improved under a current request for high-quality digital images. For example, digital still camera has several mega pixels. Although a video camera has the higher frame-rate, the resolution of a video camera is lower than that of still camera. Thus, the high-resolution is incompatible with the high frame rate of ordinary cameras in market. It is difficult to solve this problem by a single sensor, since it comes from physical limitation of the pixel transfer rate. In this paper, we propose a multi-sensor camera for capturing a resolution and frame-rate enhanced video. Common multi-CCDs camera, such as 3CCD color camera, has same CCD for capturing different spectral information. Our approach is to use different spatio-temporal resolution sensors in a single camera cabinet for capturing higher resolution and frame-rate information separately. We build a prototype camera which can capture high-resolution (2588×1958 pixels, 3.75 fps) and high frame-rate (500×500, 90 fps) videos. We also proposed the calibration method for the camera. As one of the application of the camera, we demonstrate an enhanced video (2128×1952 pixels, 90 fps) generated from the captured videos for showing the utility of the camera.

Test of CCD Precision Limits for Differential Photometry

NASA Technical Reports Server (NTRS)

Robinson, L. B.; Wei, M. Z.; Borucki, W. J.; Dunham, E. W.; Ford, C. H.; Granados, A. F.

1995-01-01

Results of tests to demonstrate the very high differential-photometric stability of CCD light sensors are presented. The measurements reported here demonstrate that in a controlled laboratory environment, a front-illuminated CCD can provide differential-photometric measurements with reproducible precision approaching one part in 10(exp 5). Practical limitations to the precision of differential-photometric measurements with CCDs and implications for spaceborne applications are discussed.

Toolkit for testing scientific CCD cameras

NASA Astrophysics Data System (ADS)

Uzycki, Janusz; Mankiewicz, Lech; Molak, Marcin; Wrochna, Grzegorz

2006-03-01

The CCD Toolkit (1) is a software tool for testing CCD cameras which allows to measure important characteristics of a camera like readout noise, total gain, dark current, 'hot' pixels, useful area, etc. The application makes a statistical analysis of images saved in files with FITS format, commonly used in astronomy. A graphical interface is based on the ROOT package, which offers high functionality and flexibility. The program was developed in a way to ensure future compatibility with different operating systems: Windows and Linux. The CCD Toolkit was created for the "Pie of the Sky" project collaboration (2).

Low temperature multi-alkali photocathode processing technique for sealed intensified CCD tubes

NASA Technical Reports Server (NTRS)

Doliber, D. L.; Dozier, E. E.; Wenzel, H.; Beaver, E. A.; Hier, R. G.

1989-01-01

A low temperature photocathode process has been used to fabricate an intensified CCD visual photocathode image tube, by incorporating a thinned, backside-illumined CCD as the target anode of a digicon tube of Hubble Space Telescope (HST) design. The CCD digicon tube employs the HST's sodium bialkali photocathode and MgF2 substrate, thereby allowing a direct photocathode quantum efficiency comparison between photocathodes produced by the presently employed low temperature process and those of the conventional high temperature process. Attention is given to the processing chamber used, as well as the details of gas desorption and photocathode processing.

Enhancement of sun-tracking with optoelectronic devices

NASA Astrophysics Data System (ADS)

Wu, Jiunn-Chi

2015-09-01

Sun-tracking is one of the most challenging tasks in implementing CPV. In order to justify the additional complexity of sun-tracking, careful assessment of performance of CPV by monitoring the performance of sun-tracking is vital. Measurement of accuracy of sun-tracking is one of the important tasks in an outdoor test. This study examines techniques with three optoelectronic devices (i.e. position sensitive device (PSD), CCD and webcam). Outdoor measurements indicated that during sunny days (global horizontal insolation (GHI) > 700 W/m2), three devices recorded comparable tracking accuracy of 0.16˜0.3°. The method using a PSD has fastest sampling rate and is able to detect the sun's position without additional image processing. Yet, it cannot identify the sunlight effectively during low insolation. The techniques with a CCD and a webcam enhance the accuracy of centroid of sunlight via the optical lens and image processing. The image quality acquired using a webcam and a CCD is comparable but the webcam is more affordable than that of CCD because it can be assembled with consumer-graded products.

SU-E-T-161: SOBP Beam Analysis Using Light Output of Scintillation Plate Acquired by CCD Camera.

PubMed

Cho, S; Lee, S; Shin, J; Min, B; Chung, K; Shin, D; Lim, Y; Park, S

2012-06-01

To analyze Bragg-peak beams in SOBP (spread-out Bragg-peak) beam using CCD (charge-coupled device) camera - scintillation screen system. We separated each Bragg-peak beam using light output of high sensitivity scintillation material acquired by CCD camera and compared with Bragg-peak beams calculated by Monte Carlo simulation. In this study, CCD camera - scintillation screen system was constructed with a high sensitivity scintillation plate (Gd2O2S:Tb) and a right-angled prismatic PMMA phantom, and a Marlin F-201B, EEE-1394 CCD camera. SOBP beam irradiated by the double scattering mode of a PROTEUS 235 proton therapy machine in NCC is 8 cm width, 13 g/cm 2 range. The gain, dose rate and current of this beam is 50, 2 Gy/min and 70 nA, respectively. Also, we simulated the light output of scintillation plate for SOBP beam using Geant4 toolkit. We evaluated the light output of high sensitivity scintillation plate according to intergration time (0.1 - 1.0 sec). The images of CCD camera during the shortest intergration time (0.1 sec) were acquired automatically and randomly, respectively. Bragg-peak beams in SOBP beam were analyzed by the acquired images. Then, the SOBP beam used in this study was calculated by Geant4 toolkit and Bragg-peak beams in SOBP beam were obtained by ROOT program. The SOBP beam consists of 13 Bragg-peak beams. The results of experiment were compared with that of simulation. We analyzed Bragg-peak beams in SOBP beam using light output of scintillation plate acquired by CCD camera and compared with that of Geant4 simulation. We are going to study SOBP beam analysis using more effective the image acquisition technique. © 2012 American Association of Physicists in Medicine.

Molecular cloning and characterization of cDNAs encoding carotenoid cleavage dioxygenase in bitter melon (Momordica charantia).

PubMed

Tuan, Pham Anh; Park, Sang Un

2013-01-01

Carotenoid cleavage dioxygenases (CCDs) are a family of enzymes that catalyze the oxidative cleavage of carotenoids at various chain positions to form a broad spectrum of apocarotenoids, including aromatic substances, pigments and phytohormones. Using the rapid amplification of cDNA ends (RACE) PCR method, we isolated three cDNA-encoding CCDs (McCCD1, McCCD4, and McNCED) from Momordica charantia. Amino acid sequence alignments showed that they share high sequence identity with other orthologous genes. Quantitative real-time RT PCR (reverse transcriptase PCR) analysis revealed that the expression of McCCD1 and McCCD4 was highest in flowers, and lowest in roots and old leaves (O-leaves). During fruit maturation, the two genes displayed differential expression, with McCCD1 peaking at mid-stage maturation while McCCD4 showed the lowest expression at that stage. The mRNA expression level of McNCED, a key enzyme involved in abscisic acid (ABA) biosynthesis, was high during fruit maturation and further increased at the beginning of seed germination. When first-leaf stage plants of M. charantia were exposed to dehydration stress, McNCED mRNA expression was induced primarily in the leaves and, to a lesser extend, in roots and stems. McNCED expression was also induced by high temperature and salinity, while treatment with exogenous ABA led to a decrease. These results should be helpful in determining the substrates and cleavage sites catalyzed by CCD genes in M. charantia, and also in defining the roles of CCDs in growth and development, and in the plant's response to environmental stress. Copyright © 2012 Elsevier GmbH. All rights reserved.

A Comprehensive Approach to Phonon Control for Enhanced Device Performance

DTIC Science & Technology

2006-07-12

substantially on parameters of the transit space . 300 kV/cm (drift region) but also specifically on the AC com- >, k\\/cm ponent of the tunnel emission... reseracher to grow well-controlled and high quality -, N2/Ar: V 35x 10 (old) multilayers and heterostructures, resulting in record electron . V 3 x 109...reflected from the sample, back through the viewport, and into a CCD camera. Monitoring fractional changes in the spacing between these re- flected spots

Deep Sky Imaging: Workflow 2

NASA Astrophysics Data System (ADS)

Schedler, Johannes

As astrophotographers we are living in a golden age. In recent years CCD technology and the quality of amateur telescopes have reached a level of perfection, giving amateurs the chance to produce images rivaling those taken from mountaintops by large professional systems as recently as two decades ago. However hardware and good imaging location is only a part of the game. A high level of skill with image processing can offer amateurs an edge and provide a chance to compensate for the limited aperture of our telescopes.

Multicolor CCD photometry of the open cluster NGC 752

NASA Astrophysics Data System (ADS)

Bartašiūtė, Stanislava; Janusz, Robert; Boyle, Richard P.; Philip, A. G. Davis; Deveikis, Viktoras

2010-01-01

We obtained CCD observations of the open cluster NGC 752 with the 1.8m Vatican Advanced Technology Telescope (Mt. Graham, Arizona) with a 4K CCD camera and eight intermediate-band filters of the Stromvil (Strömgren + Vilnius) system. Four 12‧ × 12‧ fields were observed, covering the central part of the cluster. The good-quality multicolor data made it possible to obtain precise estimates of distance moduli, metallicity and foreground reddening for individual stars down to the limiting magnitude, V = 17.5, enabling photometric identification of faint cluster members. The new observations provide an extension of the lower main sequence to three magnitudes beyond the previous (photographic) limit. A relatively small number of photometric members identified at fainter magnitudes seems to be indicative of actual dissolution of the cluster from the low-mass end.

Automatic target detection using binary template matching

NASA Astrophysics Data System (ADS)

Jun, Dong-San; Sun, Sun-Gu; Park, HyunWook

2005-03-01

This paper presents a new automatic target detection (ATD) algorithm to detect targets such as battle tanks and armored personal carriers in ground-to-ground scenarios. Whereas most ATD algorithms were developed for forward-looking infrared (FLIR) images, we have developed an ATD algorithm for charge-coupled device (CCD) images, which have superior quality to FLIR images in daylight. The proposed algorithm uses fast binary template matching with an adaptive binarization, which is robust to various light conditions in CCD images and saves computation time. Experimental results show that the proposed method has good detection performance.

STK: A new CCD camera at the University Observatory Jena

NASA Astrophysics Data System (ADS)

Mugrauer, M.; Berthold, T.

2010-04-01

The Schmidt-Teleskop-Kamera (STK) is a new CCD-imager, which is operated since begin of 2009 at the University Observatory Jena. This article describes the main characteristics of the new camera. The properties of the STK detector, the astrometry and image quality of the STK, as well as its detection limits at the 0.9 m telescope of the University Observatory Jena are presented. Based on observations obtained with telescopes of the University Observatory Jena, which is operated by the Astrophysical Institute of the Friedrich-Schiller-University.

Investigation of solar active regions at high resolution by balloon flights of the solar optical universal polarimeter, extended definition phase

NASA Technical Reports Server (NTRS)

Tarbell, Theodore D.

1993-01-01

Technical studies of the feasibility of balloon flights of the former Spacelab instrument, the Solar Optical Universal Polarimeter, with a modern charge-coupled device (CCD) camera, to study the structure and evolution of solar active regions at high resolution, are reviewed. In particular, different CCD cameras were used at ground-based solar observatories with the SOUP filter, to evaluate their performance and collect high resolution images. High resolution movies of the photosphere and chromosphere were successfully obtained using four different CCD cameras. Some of this data was collected in coordinated observations with the Yohkoh satellite during May-July, 1992, and they are being analyzed scientifically along with simultaneous X-ray observations.

Sensors for 3D Imaging: Metric Evaluation and Calibration of a CCD/CMOS Time-of-Flight Camera.

PubMed

Chiabrando, Filiberto; Chiabrando, Roberto; Piatti, Dario; Rinaudo, Fulvio

2009-01-01

3D imaging with Time-of-Flight (ToF) cameras is a promising recent technique which allows 3D point clouds to be acquired at video frame rates. However, the distance measurements of these devices are often affected by some systematic errors which decrease the quality of the acquired data. In order to evaluate these errors, some experimental tests on a CCD/CMOS ToF camera sensor, the SwissRanger (SR)-4000 camera, were performed and reported in this paper. In particular, two main aspects are treated: the calibration of the distance measurements of the SR-4000 camera, which deals with evaluation of the camera warm up time period, the distance measurement error evaluation and a study of the influence on distance measurements of the camera orientation with respect to the observed object; the second aspect concerns the photogrammetric calibration of the amplitude images delivered by the camera using a purpose-built multi-resolution field made of high contrast targets.

Fabrication of Robust, Flat, Thinned, UV-Imaging CCDs

NASA Technical Reports Server (NTRS)

Grunthaner, Paula; Elliott, Stythe; Jones, Todd; Nikzad, Shouleh

2004-01-01

An improved process that includes a high-temperature bonding subprocess has been developed to enable the fabrication of robust, flat, silicon-based charge-coupled devices (CCDs) for imaging in ultraviolet (UV) light and/or for detecting low-energy charged particles. The CCDs in question are devices on which CCD circuitry has already been formed and have been thinned for backsurface illumination. These CCDs may be delta doped, and aspects of this type of CCD have been described in several prior articles in NASA Tech Briefs. Unlike prior low-temperature bonding subprocesses based on the use of epoxies or waxes, the high-temperature bonding subprocess is compatible with the deltadoping process as well as with other CCD-fabrication processes. The present improved process and its bonding, thinning, and delta-doping subprocesses, are characterized as postfabrication processes because they are undertaken after the fabrication of CCD circuitry on the front side of a full-thickness silicon substrate. In a typical case, it is necessary to reduce the thickness of the CCD to between 10 and 20 m in order to take advantage of back-side illumination and in order to perform delta doping and/or other back-side treatment to enhance the quantum efficiency. In the prior approach to the fabrication of back-side-illuminated CCDs, the thinning subprocess turned each CCD into a free-standing membrane that was fragile and tended to become wrinkled. In the present improved process, prior to thinning and delta doping, a CCD is bonded on its front side to a silicon substrate that has been prefabricated to include cutouts to accommodate subsequent electrical connections to bonding pads on the CCD circuitry. The substrate provides structural support to increase ruggedness and maintain flatness. At the beginning of this process, the back side of a CCD as fabricated on a full-thickness substrate is polished. Silicon nitride is deposited on the back side, opposite the bonding pads on the front side, in order to define a relatively thick frame. The portion of the CCD not covered by the frame is the portion to be thinned by etching.

Dynamic imaging with a triggered and intensified CCD camera system in a high-intensity neutron beam

NASA Astrophysics Data System (ADS)

Vontobel, P.; Frei, G.; Brunner, J.; Gildemeister, A. E.; Engelhardt, M.

2005-04-01

When time-dependent processes within metallic structures should be inspected and visualized, neutrons are well suited due to their high penetration through Al, Ag, Ti or even steel. Then it becomes possible to inspect the propagation, distribution and evaporation of organic liquids as lubricants, fuel or water. The principle set-up of a suited real-time system was implemented and tested at the radiography facility NEUTRA of PSI. The highest beam intensity there is 2×107 cm s, which enables to observe sequences in a reasonable time and quality. The heart of the detection system is the MCP intensified CCD camera PI-Max with a Peltier cooled chip (1300×1340 pixels). The intensifier was used for both gating and image enhancement, where as the information was accumulated over many single frames on the chip before readout. Although, a 16-bit dynamic range is advertised by the camera manufacturers, it must be less due to the inherent noise level from the intensifier. The obtained result should be seen as the starting point to go ahead to fit the different requirements of car producers in respect to fuel injection, lubricant distribution, mechanical stability and operation control. Similar inspections will be possible for all devices with repetitive operation principle. Here, we report about two measurements dealing with the lubricant distribution in a running motorcycle motor turning at 1200 rpm. We were monitoring the periodic stationary movements of piston, valves and camshaft with a micro-channel plate intensified CCD camera system (PI-Max 1300RB, Princeton Instruments) triggered at exactly chosen time points.

CCD charge collection efficiency and the photon transfer technique

NASA Technical Reports Server (NTRS)

Janesick, J.; Klaasen, K.; Elliott, T.

1985-01-01

The charge-coupled device (CCD) has shown unprecendented performance as a photon detector in the areas of spectral response, charge transfer, and readout noise. Recent experience indicates, however, that the full potential for the CCD's charge collection efficiency (CCE) lies well beyond that which is realized in currently available devices. A definition of CCE performance is presented and a standard test tool (the photon transfer technique) for measuring and optimizing this important CCD parameter is introduced. CCE characteristics for different types of CCDs are compared; the primary limitations in achieving high CCE performance are discussed, and the prospects for future improvement are outlined.

Development of a CCD array as an imaging detector for advanced X-ray astrophysics facilities

NASA Technical Reports Server (NTRS)

Schwartz, D. A.

1981-01-01

The development of a charge coupled device (CCD) X-ray imager for a large aperture, high angular resolution X-ray telescope is discussed. Existing CCDs were surveyed and three candidate concepts were identified. An electronic camera control and computer interface, including software to drive a Fairchild 211 CCD, is described. In addition a vacuum mounting and cooling system is discussed. Performance data for the various components are given.

Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting

PubMed Central

2014-01-01

Background In saffron (Crocus sativus), new corms develop at the base of every shoot developed from the maternal corm, a globular underground storage stem. Since the degree of bud sprouts influences the number and size of new corms, and strigolactones (SLs) suppress growth of pre-formed axillary bud, it was considered appropriate to investigate SL involvement in physiology and molecular biology in saffron. We focused on two of the genes within the SL pathway, CCD7 and CCD8, encoding carotenoid cleavage enzymes required for the production of SLs. Results The CsCCD7 and CsCCD8 genes are the first ones isolated and characterized from a non-grass monocotyledonous plant. CsCCD7 and CsCCD8 expression showed some overlapping, although they were not identical. CsCCD8 was highly expressed in quiescent axillary buds and decapitation dramatically reduced its expression levels, suggesting its involvement in the suppression of axillary bud outgrowth. Furthermore, in vitro experiments showed also the involvement of auxin, cytokinin and jasmonic acid on the sprouting of axillary buds from corms in which the apical bud was removed. In addition, CsCCD8 expression, but not CsCCD7, was higher in the newly developed vascular tissue of axillary buds compared to the vascular tissue of the apical bud. Conclusions We showed that production and transport of auxin in saffron corms could act synergistically with SLs to arrest the outgrowth of the axillary buds, similar to the control of above-ground shoot branching. In addition, jasmonic acid seems to play a prominent role in bud dormancy in saffron. While cytokinins from roots promote bud outgrowth. In addition the expression results of CsCCD8 suggest that SLs could positively regulate procambial activity and the development of new vascular tissues connecting leaves with the mother corm. PMID:24947472

High-speed high-resolution epifluorescence imaging system using CCD sensor and digital storage for neurobiological research

NASA Astrophysics Data System (ADS)

Takashima, Ichiro; Kajiwara, Riichi; Murano, Kiyo; Iijima, Toshio; Morinaka, Yasuhiro; Komobuchi, Hiroyoshi

2001-04-01

We have designed and built a high-speed CCD imaging system for monitoring neural activity in an exposed animal cortex stained with a voltage-sensitive dye. Two types of custom-made CCD sensors were developed for this system. The type I chip has a resolution of 2664 (H) X 1200 (V) pixels and a wide imaging area of 28.1 X 13.8 mm, while the type II chip has 1776 X 1626 pixels and an active imaging area of 20.4 X 18.7 mm. The CCD arrays were constructed with multiple output amplifiers in order to accelerate the readout rate. The two chips were divided into either 24 (I) or 16 (II) distinct areas that were driven in parallel. The parallel CCD outputs were digitized by 12-bit A/D converters and then stored in the frame memory. The frame memory was constructed with synchronous DRAM modules, which provided a capacity of 128 MB per channel. On-chip and on-memory binning methods were incorporated into the system, e.g., this enabled us to capture 444 X 200 pixel-images for periods of 36 seconds at a rate of 500 frames/second. This system was successfully used to visualize neural activity in the cortices of rats, guinea pigs, and monkeys.

Multi-modal diffuse optical techniques for breast cancer neoadjuvant chemotherapy monitoring (Conference Presentation)

NASA Astrophysics Data System (ADS)

Cochran, Jeffrey M.; Busch, David R.; Ban, Han Y.; Kavuri, Venkaiah C.; Schweiger, Martin J.; Arridge, Simon R.; Yodh, Arjun G.

2017-02-01

We present high spatial density, multi-modal, parallel-plate Diffuse Optical Tomography (DOT) imaging systems for the purpose of breast tumor detection. One hybrid instrument provides time domain (TD) and continuous wave (CW) DOT at 64 source fiber positions. The TD diffuse optical spectroscopy with PMT- detection produces low-resolution images of absolute tissue scattering and absorption while the spatially dense array of CCD-coupled detector fibers (108 detectors) provides higher-resolution CW images of relative tissue optical properties. Reconstruction of the tissue optical properties, along with total hemoglobin concentration and tissue oxygen saturation, is performed using the TOAST software suite. Comparison of the spatially-dense DOT images and MR images allows for a robust validation of DOT against an accepted clinical modality. Additionally, the structural information from co-registered MR images is used as a spatial prior to improve the quality of the functional optical images and provide more accurate quantification of the optical and hemodynamic properties of tumors. We also present an optical-only imaging system that provides frequency domain (FD) DOT at 209 source positions with full CCD detection and incorporates optical fringe projection profilometry to determine the breast boundary. This profilometry serves as a spatial constraint, improving the quality of the DOT reconstructions while retaining the benefits of an optical-only device. We present initial images from both human subjects and phantoms to display the utility of high spatial density data and multi-modal information in DOT reconstruction with the two systems.

Pathogen webs in collapsing honey bee colonies.

PubMed

Cornman, R Scott; Tarpy, David R; Chen, Yanping; Jeffreys, Lacey; Lopez, Dawn; Pettis, Jeffery S; vanEngelsdorp, Dennis; Evans, Jay D

2012-01-01

Recent losses in honey bee colonies are unusual in their severity, geographical distribution, and, in some cases, failure to present recognized characteristics of known disease. Domesticated honey bees face numerous pests and pathogens, tempting hypotheses that colony collapses arise from exposure to new or resurgent pathogens. Here we explore the incidence and abundance of currently known honey bee pathogens in colonies suffering from Colony Collapse Disorder (CCD), otherwise weak colonies, and strong colonies from across the United States. Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies. Pathogen loads were highly covariant in CCD but not control hives, suggesting that CCD colonies rapidly become susceptible to a diverse set of pathogens, or that co-infections can act synergistically to produce the rapid depletion of workers that characterizes the disorder. We also tested workers from a CCD-free apiary to confirm that significant positive correlations among pathogen loads can develop at the level of individual bees and not merely as a secondary effect of CCD. This observation and other recent data highlight pathogen interactions as important components of bee disease. Finally, we used deep RNA sequencing to further characterize microbial diversity in CCD and non-CCD hives. We identified novel strains of the recently described Lake Sinai viruses (LSV) and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD. The results are discussed with respect to host-parasite interactions and other environmental stressors of honey bees.

Modeling the impact of preflushing on CTE in proton irradiated CCD-based detectors

NASA Astrophysics Data System (ADS)

Philbrick, R. H.

2002-04-01

A software model is described that performs a "real world" simulation of the operation of several types of charge-coupled device (CCD)-based detectors in order to accurately predict the impact that high-energy proton radiation has on image distortion and modulation transfer function (MTF). The model was written primarily to predict the effectiveness of vertical preflushing on the custom full frame CCD-based detectors intended for use on the proposed Kepler Discovery mission, but it is capable of simulating many other types of CCD detectors and operating modes as well. The model keeps track of the occupancy of all phosphorous-silicon (P-V), divacancy (V-V) and oxygen-silicon (O-V) defect centers under every CCD electrode over the entire detector area. The integrated image is read out by simulating every electrode-to-electrode charge transfer in both the vertical and horizontal CCD registers. A signal level dependency on the capture and emission of signal is included and the current state of each electrode (e.g., barrier or storage) is considered when distributing integrated and emitted signal. Options for performing preflushing, preflashing, and including mini-channels are available on both the vertical and horizontal CCD registers. In addition, dark signal generation and image transfer smear can be selectively enabled or disabled. A comparison of the charge transfer efficiency (CTE) data measured on the Hubble space telescope imaging spectrometer (STIS) CCD with the CTE extracted from model simulations of the STIS CCD show good agreement.

The development of a multifunction lens test instrument by using computer aided variable test patterns

NASA Astrophysics Data System (ADS)

Chen, Chun-Jen; Wu, Wen-Hong; Huang, Kuo-Cheng

2009-08-01

A multi-function lens test instrument is report in this paper. This system can evaluate the image resolution, image quality, depth of field, image distortion and light intensity distribution of the tested lens by changing the tested patterns. This system consists of a tested lens, a CCD camera, a linear motorized stage, a system fixture, an observer LCD monitor, and a notebook for pattern providing. The LCD monitor displays a serious of specified tested patterns sent by the notebook. Then each displayed pattern goes through the tested lens and images in the CCD camera sensor. Consequently, the system can evaluate the performance of the tested lens by analyzing the image of CCD camera with special designed software. The major advantage of this system is that it can complete whole test quickly without interruption due to part replacement, because the tested patterns are statically displayed on monitor and controlled by the notebook.

Development of a 300,000-pixel ultrahigh-speed high-sensitivity CCD

NASA Astrophysics Data System (ADS)

Ohtake, H.; Hayashida, T.; Kitamura, K.; Arai, T.; Yonai, J.; Tanioka, K.; Maruyama, H.; Etoh, T. Goji; Poggemann, D.; Ruckelshausen, A.; van Kuijk, H.; Bosiers, Jan T.

2006-02-01

We are developing an ultrahigh-speed, high-sensitivity broadcast camera that is capable of capturing clear, smooth slow-motion videos even where lighting is limited, such as at professional baseball games played at night. In earlier work, we developed an ultrahigh-speed broadcast color camera1) using three 80,000-pixel ultrahigh-speed, highsensitivity CCDs2). This camera had about ten times the sensitivity of standard high-speed cameras, and enabled an entirely new style of presentation for sports broadcasts and science programs. Most notably, increasing the pixel count is crucially important for applying ultrahigh-speed, high-sensitivity CCDs to HDTV broadcasting. This paper provides a summary of our experimental development aimed at improving the resolution of CCD even further: a new ultrahigh-speed high-sensitivity CCD that increases the pixel count four-fold to 300,000 pixels.

Fabrication and transport property of artificial structure of CNTs using SPM nano-manipulation

NASA Astrophysics Data System (ADS)

Maejima, K.; Kida, M.; Yaguchi, Y.; Sudo, K.; Kawamura, T.; Morimoto, T.; Aoki, N.; Ochiai, Y.

2007-04-01

We have established a novel manipulation technique using a glass-micro capillary under a high-resolution CCD microscope so far. Two isolated multi-wall carbon nanotubes (MWNTs) are settled to form a well-aligned cross structure. Recently, we have tried to develop a fine manipulation system using a scanning probe microscope with a silicon cantilever. Therefore, thinner high-quality MWNTs (˜10 nm in diameter) can be utilized in this system. At the junction, we have observed weak localization and Fano-like-effect, zero bias anomaly whose traces were visible even at room temperature with thick MWNTs (˜100 nm in diameter). On the other hand, with thinner high-quality MWNTs (˜10 nm in diameter), we have observed also anomalous I-V characteristic and Altshuler-Aronov-Spivak-like magneto-oscillations at low temperature in the nano-space transport.

TH-CD-201-10: Highly Efficient Synchronized High-Speed Scintillation Camera System for Measuring Proton Range, SOBP and Dose Distributions in a 2D-Plane

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goddu, S; Sun, B; Grantham, K

2016-06-15

Purpose: Proton therapy (PT) delivery is complex and extremely dynamic. Therefore, quality assurance testing is vital, but highly time-consuming. We have developed a High-Speed Scintillation-Camera-System (HS-SCS) for simultaneously measuring multiple beam characteristics. Methods: High-speed camera was placed in a light-tight housing and dual-layer neutron shield. HS-SCS is synchronized with a synchrocyclotron to capture individual proton-beam-pulses (PBPs) at ∼504 frames/sec. The PBPs from synchrocyclotron trigger the HS-SCS to open its shutter for programmed exposure-time. Light emissions within 30×30×5cm3 plastic-scintillator (BC-408) were captured by a CCD-camera as individual images revealing dose-deposition in a 2D-plane with a resolution of 0.7mm for range andmore » SOBP measurements and 1.67mm for profiles. The CCD response as well as signal to noise ratio (SNR) was characterized for varying exposure times, gains for different light intensities using a TV-Optoliner system. Software tools were developed to analyze ∼5000 images to extract different beam parameters. Quenching correction-factors were established by comparing scintillation Bragg-Peaks with water scanned ionization-chamber measurements. Quenching corrected Bragg-peaks were integrated to ascertain proton-beam range (PBR), width of Spared-Out-Bragg-Peak (MOD) and distal.« less

WIYN bench upgrade: a revitalized spectrograph

NASA Astrophysics Data System (ADS)

Bershady, M.; Barden, S.; Blanche, P.-A.; Blanco, D.; Corson, C.; Crawford, S.; Glaspey, J.; Habraken, S.; Jacoby, G.; Keyes, J.; Knezek, P.; Lemaire, P.; Liang, M.; McDougall, E.; Poczulp, G.; Sawyer, D.; Westfall, K.; Willmarth, D.

2008-07-01

We describe the redesign and upgrade of the versatile fiber-fed Bench Spectrograph on the WIYN 3.5m telescope. The spectrograph is fed by either the Hydra multi-object positioner or integral-field units (IFUs) at two other ports, and can be configured with an adjustable camera-collimator angle to use low-order and echelle gratings. The upgrade, including a new collimator, charge-coupled device (CCD) and modern controller, and volume-phase holographic gratings (VPHG), has high performance-to-cost ratio by combining new technology with a system reconfiguration that optimizes throughput while utilizing as much of the existing instrument as possible. A faster, all-refractive collimator enhances throughput by 60%, nearly eliminates the slit-function due to vignetting, and improves image quality to maintain instrumental resolution. Two VPH gratings deliver twice the diffraction efficiency of existing surface-relief gratings: A 740 l/mm grating (float-glass and post-polished) used in 1st and 2nd-order, and a large 3300 l/mm grating (spectral resolution comparable to the R2 echelle). The combination of collimator, high-quantum efficiency (QE) CCD, and VPH gratings yields throughput gain-factors of up to 3.5.

Exploration of multidimensional interactive classroom teaching for CCD principle and application course

NASA Astrophysics Data System (ADS)

Fu, Xinghu; Tan, Ailing; Zhang, Baojun; Fu, Guangwei; Bi, Weihong

2017-08-01

The CCD principle and application course is professional and comprehensive. It involves many subject contents. The course content includes eight aspects. In order to complete the teaching tasks within a limited time, improve the classroom teaching quality and prompt students master the course content faster and better, so the multidimensional interactive classroom teaching is proposed. In the teaching practice, the interactive relationship between the frontier science, scientific research project, living example and classroom content is researched detailedly. Finally, it has been proved practically that the proposed multidimensional interactive classroom teaching can achieved good teaching effect.

Vacuum compatible miniature CCD camera head

DOEpatents

Conder, Alan D.

2000-01-01

A charge-coupled device (CCD) camera head which can replace film for digital imaging of visible light, ultraviolet radiation, and soft to penetrating x-rays, such as within a target chamber where laser produced plasmas are studied. The camera head is small, capable of operating both in and out of a vacuum environment, and is versatile. The CCD camera head uses PC boards with an internal heat sink connected to the chassis for heat dissipation, which allows for close(0.04" for example) stacking of the PC boards. Integration of this CCD camera head into existing instrumentation provides a substantial enhancement of diagnostic capabilities for studying high energy density plasmas, for a variety of military industrial, and medical imaging applications.

Measurements of 161 Double Stars With a High-Speed CCD: The Winter/Spring 2017 Observing Program at Brilliant Sky Observatory, Part 2

NASA Astrophysics Data System (ADS)

Harshaw, Richard

2018-04-01

In the winter and spring of 2017, an aggressive observing program of measuring close double stars with speckle interferometry and CCD imaging was undertaken at Brilliant Sky Observatory, my observing site in Cave Creek, Arizona. A total of 596 stars were observed, 8 of which were rejected for various reasons, leaving 588 pairs. Of these, 427 were observed and measured with speckle interferometry, while the remaining 161 were measured with a CCD. This paper reports the results of the observations of the 161 CCD cases. A separate paper in this issue will report the speckle measurements of the 427 other pairs.

A CCD experimental platform for large telescope in Antarctica based on FPGA

NASA Astrophysics Data System (ADS)

Zhu, Yuhua; Qi, Yongjun

2014-07-01

The CCD , as a detector , is one of the important components of astronomical telescopes. For a large telescope in Antarctica, a set of CCD detector system with large size, high sensitivity and low noise is indispensable. Because of the extremely low temperatures and unattended, system maintenance and software and hardware upgrade become hard problems. This paper introduces a general CCD controller experiment platform, using Field programmable gate array FPGA, which is, in fact, a large-scale field reconfigurable array. Taking the advantage of convenience to modify the system, construction of driving circuit, digital signal processing module, network communication interface, control algorithm validation, and remote reconfigurable module may realize. With the concept of integrated hardware and software, the paper discusses the key technology of building scientific CCD system suitable for the special work environment in Antarctica, focusing on the method of remote reconfiguration for controller via network and then offering a feasible hardware and software solution.

High resolution biomedical imaging system with direct detection of x-rays via a charge coupled device

DOEpatents

Atac, M.; McKay, T.A.

1998-04-21

An imaging system is provided for direct detection of x-rays from an irradiated biological tissue. The imaging system includes an energy source for emitting x-rays toward the biological tissue and a charge coupled device (CCD) located immediately adjacent the biological tissue and arranged transverse to the direction of irradiation along which the x-rays travel. The CCD directly receives and detects the x-rays after passing through the biological tissue. The CCD is divided into a matrix of cells, each of which individually stores a count of x-rays directly detected by the cell. The imaging system further includes a pattern generator electrically coupled to the CCD for reading a count from each cell. A display device is provided for displaying an image representative of the count read by the pattern generator from the cells of the CCD. 13 figs.

High resolution biomedical imaging system with direct detection of x-rays via a charge coupled device

DOEpatents

Atac, Muzaffer; McKay, Timothy A.

1998-01-01

An imaging system is provided for direct detection of x-rays from an irradiated biological tissue. The imaging system includes an energy source for emitting x-rays toward the biological tissue and a charge coupled device (CCD) located immediately adjacent the biological tissue and arranged transverse to the direction of irradiation along which the x-rays travel. The CCD directly receives and detects the x-rays after passing through the biological tissue. The CCD is divided into a matrix of cells, each of which individually stores a count of x-rays directly detected by the cell. The imaging system further includes a pattern generator electrically coupled to the CCD for reading a count from each cell. A display device is provided for displaying an image representative of the count read by the pattern generator from the cells of the CCD.

A method of immediate detection of objects with a near-zero apparent motion in series of CCD-frames

NASA Astrophysics Data System (ADS)

Savanevych, V. E.; Khlamov, S. V.; Vavilova, I. B.; Briukhovetskyi, A. B.; Pohorelov, A. V.; Mkrtichian, D. E.; Kudak, V. I.; Pakuliak, L. K.; Dikov, E. N.; Melnik, R. G.; Vlasenko, V. P.; Reichart, D. E.

2018-01-01

The paper deals with a computational method for detection of the solar system minor bodies (SSOs), whose inter-frame shifts in series of CCD-frames during the observation are commensurate with the errors in measuring their positions. These objects have velocities of apparent motion between CCD-frames not exceeding three rms errors (3σ) of measurements of their positions. About 15% of objects have a near-zero apparent motion in CCD-frames, including the objects beyond the Jupiter's orbit as well as the asteroids heading straight to the Earth. The proposed method for detection of the object's near-zero apparent motion in series of CCD-frames is based on the Fisher f-criterion instead of using the traditional decision rules that are based on the maximum likelihood criterion. We analyzed the quality indicators of detection of the object's near-zero apparent motion applying statistical and in situ modeling techniques in terms of the conditional probability of the true detection of objects with a near-zero apparent motion. The efficiency of method being implemented as a plugin for the Collection Light Technology (CoLiTec) software for automated asteroids and comets detection has been demonstrated. Among the objects discovered with this plugin, there was the sungrazing comet C/2012 S1 (ISON). Within 26 min of the observation, the comet's image has been moved by three pixels in a series of four CCD-frames (the velocity of its apparent motion at the moment of discovery was equal to 0.8 pixels per CCD-frame; the image size on the frame was about five pixels). Next verification in observations of asteroids with a near-zero apparent motion conducted with small telescopes has confirmed an efficiency of the method even in bad conditions (strong backlight from the full Moon). So, we recommend applying the proposed method for series of observations with four or more frames.

Pathogen Webs in Collapsing Honey Bee Colonies

PubMed Central

Cornman, R. Scott; Tarpy, David R.; Chen, Yanping; Jeffreys, Lacey; Lopez, Dawn; Pettis, Jeffery S.; vanEngelsdorp, Dennis; Evans, Jay D.

2012-01-01

Recent losses in honey bee colonies are unusual in their severity, geographical distribution, and, in some cases, failure to present recognized characteristics of known disease. Domesticated honey bees face numerous pests and pathogens, tempting hypotheses that colony collapses arise from exposure to new or resurgent pathogens. Here we explore the incidence and abundance of currently known honey bee pathogens in colonies suffering from Colony Collapse Disorder (CCD), otherwise weak colonies, and strong colonies from across the United States. Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies. Pathogen loads were highly covariant in CCD but not control hives, suggesting that CCD colonies rapidly become susceptible to a diverse set of pathogens, or that co-infections can act synergistically to produce the rapid depletion of workers that characterizes the disorder. We also tested workers from a CCD-free apiary to confirm that significant positive correlations among pathogen loads can develop at the level of individual bees and not merely as a secondary effect of CCD. This observation and other recent data highlight pathogen interactions as important components of bee disease. Finally, we used deep RNA sequencing to further characterize microbial diversity in CCD and non-CCD hives. We identified novel strains of the recently described Lake Sinai viruses (LSV) and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD. The results are discussed with respect to host-parasite interactions and other environmental stressors of honey bees. PMID:22927991

An Overview of the CBERS-2 Satellite and Comparison of the CBERS-2 CCD Data with the L5 TM Data

NASA Technical Reports Server (NTRS)

Chandler, Gyanesh

2007-01-01

CBERS satellite carries on-board a multi sensor payload with different spatial resolutions and collection frequencies. HRCCD (High Resolution CCD Camera), IRMSS (Infrared Multispectral Scanner), and WFI (Wide-Field Imager). The CCD and the WFI camera operate in the VNIR regions, while the IRMSS operates in SWIR and thermal region. In addition to the imaging payload, the satellite carries a Data Collection System (DCS) and Space Environment Monitor (SEM).

Updating the Synchrotron Radiation Monitor at TLS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kuo, C. H.; Hsu, S. Y.; Wang, C. J.

2007-01-19

The synchrotron radiation monitor provides useful information to support routine operation and physics experiments using the beam. Precisely knowing the profile of the beam helps to improve machine performance. The synchrotron radiation monitor at the Taiwan Light Source (TLS) was recently upgraded. The optics and modeling were improved to increase the accuracy of measurement in the small beam size. A high-performance IEEE-1394 digital CCD camera was used to improve the quality of images and extend the dynamic range of measurement. The image analysis is also improved. This report summarizes status and results.

High-performance visible/UV CCD focal plane technology for spacebased applications

NASA Technical Reports Server (NTRS)

Burke, B. E.; Mountain, R. W.; Gregory, J. A.; Huang, J. C. M.; Cooper, M. J.; Savoye, E. D.; Kosicki, B. B.

1993-01-01

We describe recent technology developments aimed at large CCD imagers for space based applications in the visible and UV. Some of the principal areas of effort include work on reducing device degradation in the natural space-radiation environment, improvements in quantum efficiency in the visible and UV, and larger-device formats. One of the most serious hazards for space based CCD's operating at low signal levels is the displacement damage resulting from bombardment by energetic protons. Such damage degrades charge-transfer efficiency and increases dark current. We have achieved improved hardness to proton-induced displacement damage by selective ion implants into the CCD channel and by reduced temperature of operation. To attain high quantum efficiency across the visible and UV we have developed a technology for back-illuminated CCD's. With suitable antireflection (AR) coatings such devices have quantum efficiencies near 90 percent in the 500-700-nm band. In the UV band from 200 to 400 nm, where it is difficult to find coatings that are sufficiently transparent and can provide good matching to the high refractive index of silicon, we have been able to substantially increase the quantum efficiency using a thin film of HfO2 as an AR coating. These technology efforts were applied to a 420 x 420-pixel frame-transfer imager, and future work will be extended to a 1024 x 1024-pixel device now under development.

Combining HJ CCD, GF-1 WFV and MODIS Data to Generate Daily High Spatial Resolution Synthetic Data for Environmental Process Monitoring.

PubMed

Wu, Mingquan; Huang, Wenjiang; Niu, Zheng; Wang, Changyao

2015-08-20

The limitations of satellite data acquisition mean that there is a lack of satellite data with high spatial and temporal resolutions for environmental process monitoring. In this study, we address this problem by applying the Enhanced Spatial and Temporal Adaptive Reflectance Fusion Model (ESTARFM) and the Spatial and Temporal Data Fusion Approach (STDFA) to combine Huanjing satellite charge coupled device (HJ CCD), Gaofen satellite no. 1 wide field of view camera (GF-1 WFV) and Moderate Resolution Imaging Spectroradiometer (MODIS) data to generate daily high spatial resolution synthetic data for land surface process monitoring. Actual HJ CCD and GF-1 WFV data were used to evaluate the precision of the synthetic images using the correlation analysis method. Our method was tested and validated for two study areas in Xinjiang Province, China. The results show that both the ESTARFM and STDFA can be applied to combine HJ CCD and MODIS reflectance data, and GF-1 WFV and MODIS reflectance data, to generate synthetic HJ CCD data and synthetic GF-1 WFV data that closely match actual data with correlation coefficients (r) greater than 0.8989 and 0.8643, respectively. Synthetic red- and near infrared (NIR)-band data generated by ESTARFM are more suitable for the calculation of Normalized Different Vegetation Index (NDVI) than the data generated by STDFA.

Combining HJ CCD, GF-1 WFV and MODIS Data to Generate Daily High Spatial Resolution Synthetic Data for Environmental Process Monitoring

PubMed Central

Wu, Mingquan; Huang, Wenjiang; Niu, Zheng; Wang, Changyao

2015-01-01

The limitations of satellite data acquisition mean that there is a lack of satellite data with high spatial and temporal resolutions for environmental process monitoring. In this study, we address this problem by applying the Enhanced Spatial and Temporal Adaptive Reflectance Fusion Model (ESTARFM) and the Spatial and Temporal Data Fusion Approach (STDFA) to combine Huanjing satellite charge coupled device (HJ CCD), Gaofen satellite no. 1 wide field of view camera (GF-1 WFV) and Moderate Resolution Imaging Spectroradiometer (MODIS) data to generate daily high spatial resolution synthetic data for land surface process monitoring. Actual HJ CCD and GF-1 WFV data were used to evaluate the precision of the synthetic images using the correlation analysis method. Our method was tested and validated for two study areas in Xinjiang Province, China. The results show that both the ESTARFM and STDFA can be applied to combine HJ CCD and MODIS reflectance data, and GF-1 WFV and MODIS reflectance data, to generate synthetic HJ CCD data and synthetic GF-1 WFV data that closely match actual data with correlation coefficients (r) greater than 0.8989 and 0.8643, respectively. Synthetic red- and near infrared (NIR)-band data generated by ESTARFM are more suitable for the calculation of Normalized Different Vegetation Index (NDVI) than the data generated by STDFA. PMID:26308017

Design of area array CCD image acquisition and display system based on FPGA

NASA Astrophysics Data System (ADS)

Li, Lei; Zhang, Ning; Li, Tianting; Pan, Yue; Dai, Yuming

2014-09-01

With the development of science and technology, CCD(Charge-coupled Device) has been widely applied in various fields and plays an important role in the modern sensing system, therefore researching a real-time image acquisition and display plan based on CCD device has great significance. This paper introduces an image data acquisition and display system of area array CCD based on FPGA. Several key technical challenges and problems of the system have also been analyzed and followed solutions put forward .The FPGA works as the core processing unit in the system that controls the integral time sequence .The ICX285AL area array CCD image sensor produced by SONY Corporation has been used in the system. The FPGA works to complete the driver of the area array CCD, then analog front end (AFE) processes the signal of the CCD image, including amplification, filtering, noise elimination, CDS correlation double sampling, etc. AD9945 produced by ADI Corporation to convert analog signal to digital signal. Developed Camera Link high-speed data transmission circuit, and completed the PC-end software design of the image acquisition, and realized the real-time display of images. The result through practical testing indicates that the system in the image acquisition and control is stable and reliable, and the indicators meet the actual project requirements.

Overexpression of the rice carotenoid cleavage dioxygenase 1 gene in Golden Rice endosperm suggests apocarotenoids as substrates in planta.

PubMed

Ilg, Andrea; Yu, Qiuju; Schaub, Patrick; Beyer, Peter; Al-Babili, Salim

2010-08-01

Carotenoids are converted by carotenoid cleavage dioxygenases that catalyze oxidative cleavage reactions leading to apocarotenoids. However, apocarotenoids can also be further truncated by some members of this enzyme family. The plant carotenoid cleavage dioxygenase 1 (CCD1) subfamily is known to degrade both carotenoids and apocarotenoids in vitro, leading to different volatile compounds. In this study, we investigated the impact of the rice CCD1 (OsCCD1) on the pigmentation of Golden Rice 2 (GR2), a genetically modified rice variety accumulating carotenoids in the endosperm. For this purpose, the corresponding cDNA was introduced into the rice genome under the control of an endosperm-specific promoter in sense and anti-sense orientations. Despite high expression levels of OsCCD1 in sense plants, pigment analysis revealed carotenoid levels and patterns comparable to those of GR2, pleading against carotenoids as substrates in rice endosperm. In support, similar carotenoid contents were determined in anti-sense plants. To check whether OsCCD1 overexpressed in GR2 endosperm is active, in vitro assays were performed with apocarotenoid substrates. HPLC analysis confirmed the cleavage activity of introduced OsCCD1. Our data indicate that apocarotenoids rather than carotenoids are the substrates of OsCCD1 in planta.

Combination of Wavefunction and Density Functional Approximations for Describing Electronic Correlation

NASA Astrophysics Data System (ADS)

Garza, Alejandro J.

Perhaps the most important approximations to the electronic structure problem in quantum chemistry are those based on coupled cluster and density functional theories. Coupled cluster theory has been called the ``gold standard'' of quantum chemistry due to the high accuracy that it achieves for weakly correlated systems. Kohn-Sham density functionals based on semilocal approximations are, without a doubt, the most widely used methods in chemistry and material science because of their high accuracy/cost ratio. The root of the success of coupled cluster and density functionals is their ability to efficiently describe the dynamic part of the electron correlation. However, both traditional coupled cluster and density functional approximations may fail catastrophically when substantial static correlation is present. This severely limits the applicability of these methods to a plethora of important chemical and physical problems such as, e.g., the description of bond breaking, transition states, transition metal-, lanthanide- and actinide-containing compounds, and superconductivity. In an attempt to tackle this problem, nonstandard (single-reference) coupled cluster-based techniques that aim to describe static correlation have been recently developed: pair coupled cluster doubles (pCCD) and singlet-paired coupled cluster doubles (CCD0). The ability to describe static correlation in pCCD and CCD0 comes, however, at the expense of important amounts of dynamic correlation so that the high accuracy of standard coupled cluster becomes unattainable. Thus, the reliable and efficient description of static and dynamic correlation in a simultaneous manner remains an open problem for quantum chemistry and many-body theory in general. In this thesis, different ways to combine pCCD and CCD0 with density functionals in order to describe static and dynamic correlation simultaneously (and efficiently) are explored. The combination of wavefunction and density functional methods has a long history in quantum chemistry (practical implementations have appeared in the literature since the 1970s). However, this kind of techniques have not achieved widespread use due to problems such as double counting of correlation and the symmetry dilemma--the fact that wavefunction methods respect the symmetries of Hamiltonian, while modern functionals are designed to work with broken symmetry densities. Here, particular mathematical features of pCCD and CCD0 are exploited to avoid these problems in an efficient manner. The two resulting families of approximations, denoted as pCCD+DFT and CCD0+DFT, are shown to be able to describe static and dynamic correlation in standard benchmark calculations. Furthermore, it is also shown that CCD0+DFT lends itself to combination with correlation from the direct random phase approximation (dRPA). Inclusion of dRPA in the long-range via the technique of range-separation allows for the description of dispersion correlation, the remaining part of the correlation. Thus, when combined with the dRPA, CCD0+DFT can account for all three-types of electron correlation that are necessary to accurately describe molecular systems. Lastly, applications of CCD0+DFT to actinide chemistry are considered in this work. The accuracy of CCD0+DFT for predicting equilibrium geometries and vibrational frequencies of actinide molecules and ions is assessed and compared to that of well-established quantum chemical methods. For this purpose, the f0 actinyl series (UO2 2+, NpO 23+, PuO24+, the isoelectronic NUN, and Thorium (ThO, ThO2+) and Nobelium (NoO, NoO2) oxides are studied. It is shown that the CCD0+DFT description of these species agrees with available experimental data and is comparable with the results given by the highest-level calculations that are possible for such heavy compounds while being, at least, an order of magnitude lower in computational cost.

Experimental Evaluation of Optically Polished Aluminum Panels on the Deep Space Network's 34 Meter Antenna

NASA Technical Reports Server (NTRS)

Vilnrotter, V.

2011-01-01

The potential development of large aperture ground?based "photon bucket" optical receivers for deep space communications has received considerable attention recently. One approach currently under investigation is to polish the aluminum reflector panels of 34?meter microwave antennas to high reflectance, and accept the relatively large spotsize generated by state of?the?art polished aluminum panels. Theoretical analyses of receiving antenna pointing, temporal synchronization and data detection have been addressed in previous papers. Here we describe the experimental effort currently underway at the Deep Space Network (DSN) Goldstone Communications Complex in California, to test and verify these concepts in a realistic operational environment. Two polished aluminum panels (a standard DSN panel polished to high reflectance, and a custom designed aluminum panel with much better surface quality) have been mounted on the 34 meter research antenna at Deep?Space Station 13 (DSS?13), and a remotely controlled CCD camera with a large CCD sensor in a weather?proof container has been installed next to the subreflector, pointed directly at the custom polished panel. The point?spread function (PSF) generated by the Vertex polished panel has been determined to be smaller than the sensor of the CCD camera, hence a detailed picture of the PSF can be obtained every few seconds, and the sensor array data processed to determine the center of the intensity distribution. In addition to estimating the center coordinates, expected communications performance can also been evaluated with the recorded data. The results of preliminary pointing experiments with the Vertex polished panel receiver using the planet Jupiter to simulate the PSF generated by a deep?space optical transmitter are presented and discussed in this paper.

Design of system calibration for effective imaging

NASA Astrophysics Data System (ADS)

Varaprasad Babu, G.; Rao, K. M. M.

2006-12-01

A CCD based characterization setup comprising of a light source, CCD linear array, Electronics for signal conditioning/ amplification, PC interface has been developed to generate images at varying densities and at multiple view angles. This arrangement is used to simulate and evaluate images by Super Resolution technique with multiple overlaps and yaw rotated images at different view angles. This setup also generates images at different densities to analyze the response of the detector port wise separately. The light intensity produced by the source needs to be calibrated for proper imaging by the high sensitive CCD detector over the FOV. One approach is to design a complex integrating sphere arrangement which costs higher for such applications. Another approach is to provide a suitable intensity feed back correction wherein the current through the lamp is controlled in a closed loop arrangement. This method is generally used in the applications where the light source is a point source. The third method is to control the time of exposure inversely to the lamp variations where lamp intensity is not possible to control. In this method, light intensity during the start of each line is sampled and the correction factor is applied for the full line. The fourth method is to provide correction through Look Up Table where the response of all the detectors are normalized through the digital transfer function. The fifth method is to have a light line arrangement where the light through multiple fiber optic cables are derived from a single source and arranged them in line. This is generally applicable and economical for low width cases. In our applications, a new method wherein an inverse multi density filter is designed which provides an effective calibration for the full swath even at low light intensities. The light intensity along the length is measured, an inverse density is computed, a correction filter is generated and implemented in the CCD based Characterization setup. This paper describes certain novel techniques of design and implementation of system calibration for effective Imaging to produce better quality data product especially while handling high resolution data.

CCD centroiding analysis for Nano-JASMINE observation data

NASA Astrophysics Data System (ADS)

Niwa, Yoshito; Yano, Taihei; Araki, Hiroshi; Gouda, Naoteru; Kobayashi, Yukiyasu; Yamada, Yoshiyuki; Tazawa, Seiichi; Hanada, Hideo

2010-07-01

Nano-JASMINE is a very small satellite mission for global space astrometry with milli-arcsecond accuracy, which will be launched in 2011. In this mission, centroids of stars in CCD image frames are estimated with sub-pixel accuracy. In order to realize such a high precision centroiding an algorithm utilizing a least square method is employed. One of the advantages is that centroids can be calculated without explicit assumption of the point spread functions of stars. CCD centroiding experiment has been performed to investigate whether this data analysis is available, and centroids of artificial star images on a CCD are determined with a precision of less than 0.001 pixel. This result indicates parallaxes of stars within 300 pc from Sun can be observed in Nano-JASMINE.

Printed circuit board for a CCD camera head

DOEpatents

Conder, Alan D.

2002-01-01

A charge-coupled device (CCD) camera head which can replace film for digital imaging of visible light, ultraviolet radiation, and soft to penetrating x-rays, such as within a target chamber where laser produced plasmas are studied. The camera head is small, capable of operating both in and out of a vacuum environment, and is versatile. The CCD camera head uses PC boards with an internal heat sink connected to the chassis for heat dissipation, which allows for close (0.04" for example) stacking of the PC boards. Integration of this CCD camera head into existing instrumentation provides a substantial enhancement of diagnostic capabilities for studying high energy density plasmas, for a variety of military industrial, and medical imaging applications.

QUALITY ASSESSMENT OF CONFOCAL MICROSCOPY SLIDE-BASED SYSTEMS: INSTABLITY

EPA Science Inventory

Background: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. Methods: A num...

Characterization of a 512x512-pixel 8-output full-frame CCD for high-speed imaging

NASA Astrophysics Data System (ADS)

Graeve, Thorsten; Dereniak, Eustace L.

1993-01-01

The characterization of a 512 by 512 pixel, eight-output full frame CCD manufactured by English Electric Valve under part number CCD13 is discussed. This device is a high- resolution Silicon-based array designed for visible imaging applications at readout periods as low as two milliseconds. The characterization of the device includes mean-variance analysis to determine read noise and dynamic range, as well as charge transfer efficiency, MTF, and quantum efficiency measurements. Dark current and non-uniformity issues on a pixel-to-pixel basis and between individual outputs are also examined. The characterization of the device is restricted by hardware limitations to a one MHz pixel rate, corresponding to a 40 ms readout time. However, subsections of the device have been operated at up to an equivalent 100 frames per second. To maximize the frame rate, the CCD is illuminated by a synchronized strobe flash in between frame readouts. The effects of the strobe illumination on the imagery obtained from the device is discussed.

Colony Collapse Disorder (CCD) and bee age impact honey bee pathophysiology

PubMed Central

Traynor, Kirsten S.; Andree, Michael; Lichtenberg, Elinor M.; Chen, Yanping; Saegerman, Claude; Cox-Foster, Diana L.

2017-01-01

Honey bee (Apis mellifera) colonies continue to experience high annual losses that remain poorly explained. Numerous interacting factors have been linked to colony declines. Understanding the pathways linking pathophysiology with symptoms is an important step in understanding the mechanisms of disease. In this study we examined the specific pathologies associated with honey bees collected from colonies suffering from Colony Collapse Disorder (CCD) and compared these with bees collected from apparently healthy colonies. We identified a set of pathological physical characteristics that occurred at different rates in CCD diagnosed colonies prior to their collapse: rectum distension, Malpighian tubule iridescence, fecal matter consistency, rectal enteroliths (hard concretions), and venom sac color. The multiple differences in rectum symptomology in bees from CCD apiaries and colonies suggest effected bees had trouble regulating water. To ensure that pathologies we found associated with CCD were indeed pathologies and not due to normal changes in physical appearances that occur as an adult bee ages (CCD colonies are assumed to be composed mostly of young bees), we documented the changes in bees of different ages taken from healthy colonies. We found that young bees had much greater incidences of white nodules than older cohorts. Prevalent in newly-emerged bees, these white nodules or cellular encapsulations indicate an active immune response. Comparing the two sets of characteristics, we determined a subset of pathologies that reliably predict CCD status rather than bee age (fecal matter consistency, rectal distension size, rectal enteroliths and Malpighian tubule iridescence) and that may serve as biomarkers for colony health. In addition, these pathologies suggest that CCD bees are experiencing disrupted excretory physiology. Our identification of these symptoms is an important first step in understanding the physiological pathways that underlie CCD and factors impacting bee health. PMID:28715431

Colony Collapse Disorder (CCD) and bee age impact honey bee pathophysiology.

PubMed

vanEngelsdorp, Dennis; Traynor, Kirsten S; Andree, Michael; Lichtenberg, Elinor M; Chen, Yanping; Saegerman, Claude; Cox-Foster, Diana L

2017-01-01

Honey bee (Apis mellifera) colonies continue to experience high annual losses that remain poorly explained. Numerous interacting factors have been linked to colony declines. Understanding the pathways linking pathophysiology with symptoms is an important step in understanding the mechanisms of disease. In this study we examined the specific pathologies associated with honey bees collected from colonies suffering from Colony Collapse Disorder (CCD) and compared these with bees collected from apparently healthy colonies. We identified a set of pathological physical characteristics that occurred at different rates in CCD diagnosed colonies prior to their collapse: rectum distension, Malpighian tubule iridescence, fecal matter consistency, rectal enteroliths (hard concretions), and venom sac color. The multiple differences in rectum symptomology in bees from CCD apiaries and colonies suggest effected bees had trouble regulating water. To ensure that pathologies we found associated with CCD were indeed pathologies and not due to normal changes in physical appearances that occur as an adult bee ages (CCD colonies are assumed to be composed mostly of young bees), we documented the changes in bees of different ages taken from healthy colonies. We found that young bees had much greater incidences of white nodules than older cohorts. Prevalent in newly-emerged bees, these white nodules or cellular encapsulations indicate an active immune response. Comparing the two sets of characteristics, we determined a subset of pathologies that reliably predict CCD status rather than bee age (fecal matter consistency, rectal distension size, rectal enteroliths and Malpighian tubule iridescence) and that may serve as biomarkers for colony health. In addition, these pathologies suggest that CCD bees are experiencing disrupted excretory physiology. Our identification of these symptoms is an important first step in understanding the physiological pathways that underlie CCD and factors impacting bee health.

A CCD Spectrometer for One Dollar

NASA Astrophysics Data System (ADS)

Beaver, J.; Robert, D.

2011-09-01

We describe preliminary tests on a very low-cost system for obtaining stellar spectra for instructional use in an introductory astronomy laboratory. CCD imaging with small telescopes is now commonplace and relatively inexpensive. Giving students direct experience taking stellar spectra, however, is much more difficult, and the equipment can easily be out of reach for smaller institutions, especially if one wants to give the experience to large numbers of students. We have performed preliminary tests on an extremely low-cost (about $1.00) objective grating that can be coupled with an existing CCD camera or commercial digital single-lens reflex (DSLR) camera and a small telescope typical of introductory astronomy labs. With this equipment we believe it is possible for introductory astronomy students to take stellar spectra that are of high enough quality to distinguish between many MK spectral classes, or to determine standard B and V magnitudes. We present observational tests of this objective grating used on an 8" Schmidt-Cassegrain with a low-end, consumer DSLR camera. Some low-cost strategies for reducing the raw data are compared, with an eye toward projects ranging from individual undergraduate research projects to use by many students in a non-majors introductory astronomy lab. Toward this end we compare various trade offs between complexity of the observing and data reduction processes and the usefulness of the final results. We also describe some undergraduate astronomy education projects that this system could potentially be used for. Some of these projects could involve data-sharing collaborations between students at different institutions.

[Effects of Cangfu Congxian Decoction on Oxidative Stress in Polycystic Ovary Syndrome Patients].

PubMed

Liang, Ying; Tian, Qian-hua; Mu, Yu-xia; Du, Hui-lan

2016-06-01

To observe the effect of Cangfu Congxian Decoction (CCD) on oxidative stress in granulosa cells of polycystic ovary syndrome (PCOS) patients. Forty PCOS patients underwent in vitro fertilization-embryo transfer (IVF-ET) were assigned to the treatment group and the control group 1 according to random digit table, 20 in each group. Patients in the treatment group took CCD (200 mL, once in the morning and once in the afternoon) 2 months before IVF-ET, while those in the control group 1 took no Chinese medical decoction. Recruited were another 20 patients undergoing IVF-ET for tubal factors (as the control group 2). The clinical effect of IVF-ET were observed, including oocyte retrieval number, 2 pronuclear (2PN) fertilization rate, good quality embryo rate, clinical pregnancy rate, and ovarian hyperstimulation syndrome (OHSS) induced transplantation cancel rate. The expression of relative oxygen species (ROS) in granulosa cells was detected using cell immunofluorescence combined with confocal microscopy and FCM. Compared with the control group 1, occyte retrieval number, 2PN fertilization rate, and good quality embryo rate increased in the control group 2 and the treatment group (P <0. 05). OHSS induced transplantation cancel rate decreased in the control group 2 (P < 0.05). Fluorescence intensity of ROS decreased in the treatment group and the control group 2, as compared with the control group 1 (P < 0.01). CCD increased good quality embryo rate by down-regulating the expression of ROS protein in ovarian granulosa cells, and correcting in vivo oxidative stress.

First Results of Digital Topography Applied to Macromolecular Crystals

NASA Technical Reports Server (NTRS)

Lovelace, J.; Soares, A. S.; Bellamy, H.; Sweet, R. M.; Snell, E. H.; Borgstahl, G.

2004-01-01

An inexpensive digital CCD camera was used to record X-ray topographs directly from large imperfect crystals of cubic insulin. The topographs recorded were not as detailed as those which can be measured with film or emulsion plates but do show great promise. Six reflections were recorded using a set of finely spaced stills encompassing the rocking curve of each reflection. A complete topographic reflection profile could be digitally imaged in minutes. Interesting and complex internal structure was observed by this technique.The CCD chip used in the camera has anti-blooming circuitry and produced good data quality even when pixels became overloaded.

Astrometric Quality of the USNO CCD Astrograph (UCA)

NASA Astrophysics Data System (ADS)

Zacharias, N.

1997-05-01

The USNO 8-inch astrograph has been equipped with a Kodak 1536x1024 pixel CCD since June 1995, operating in a 570-650 nm bandpass. With 3-minute exposures well exposed images are obtained in the magnitude range R ~ 8.5 - 13.5(m) . An astrometric precision of 10 to 15 mas for those stars is estimated from frame-to-frame comparisons. External comparisons reveal an accuracy of about 15 mas for those stars in a 20' field of view. For fainter stars, the error budget is dominated by the S/N ratio, reaching ~ 100 mas at R=16(m) under good observing conditions.

Evaluation of large format electron bombarded virtual phase CCDs as ultraviolet imaging detectors

NASA Technical Reports Server (NTRS)

Opal, Chet B.; Carruthers, George R.

1989-01-01

In conjunction with an external UV-sensitive cathode, an electron-bombarded CCD may be used as a high quantum efficiency/wide dynamic range photon-counting UV detector. Results are presented for the case of a 1024 x 1024, 18-micron square pixel virtual phase CCD used with an electromagnetically focused f/2 Schmidt camera, which yields excellent simgle-photoevent discrimination and counting efficiency. Attention is given to the vacuum-chamber arrangement used to conduct system tests and the CCD electronics and data-acquisition systems employed.

A design of driving circuit for star sensor imaging camera

NASA Astrophysics Data System (ADS)

Li, Da-wei; Yang, Xiao-xu; Han, Jun-feng; Liu, Zhao-hui

2016-01-01

The star sensor is a high-precision attitude sensitive measuring instruments, which determine spacecraft attitude by detecting different positions on the celestial sphere. Imaging camera is an important portion of star sensor. The purpose of this study is to design a driving circuit based on Kodak CCD sensor. The design of driving circuit based on Kodak KAI-04022 is discussed, and the timing of this CCD sensor is analyzed. By the driving circuit testing laboratory and imaging experiments, it is found that the driving circuits can meet the requirements of Kodak CCD sensor.

Construction of a photochemical reactor combining a CCD spectrophotometer and a LED radiation source.

PubMed

Gombár, Melinda; Józsa, Éva; Braun, Mihály; Ősz, Katalin

2012-10-01

An inexpensive photoreactor using LED light sources and a fibre-optic CCD spectrophotometer as a detector was built by designing a special cell holder for standard 1.000 cm cuvettes. The use of this device was demonstrated by studying the aqueous photochemical reaction of 2,5-dichloro-1,4-benzoquinone. The developed method combines the highly quantitative data collection of CCD spectrophotometers with the possibility of illuminating the sample independently of the detecting light beam, which is a substantial improvement of the method using diode array spectrophotometers as photoreactors.

The 2006-2007 Observing Campaign On VX Hydrae

NASA Astrophysics Data System (ADS)

Templeton, Matthew R.; Samolyk, G.; Dvorak, S.; Poklar, R.; Butterworth, N.; Gerner, H. S.

2009-12-01

We present the results of the 2006-2007 observing campaign on the double-mode delta Scuti star VX Hydrae. Nearly 8800 V-band CCD observations were obtained during the two observing seasons. Although the data were taken with small telescopes (0.3-m or less, using consumer-grade CCD cameras), the data quality is very high, enabling the detection of variability at the millimagnitude level at some frequencies. Analysis of the data yields only two primary pulsation frequencies: f(0) = 4.4765 c/d, and f(1) = 5.7899 c/d. The two modes have comparable amplitude, although the amplitude of f(1) appears to have increased slightly from 2006 to 2007 by 0.01 mag. Only two pulsation modes are detected, but at least 18 additional linear combination frequencies are also clearly detected, some having amplitudes as low as 1 mmag, resulting in an incredibly rich Fourier spectrum. We discuss the evidence for amplitude variation in VX Hydrae, along with prospects for future study of this and other similar delta Scuti stars by AAVSO observers.

Control and protection of outdoor embedded camera for astronomy

NASA Astrophysics Data System (ADS)

Rigaud, F.; Jegouzo, I.; Gaudemard, J.; Vaubaillon, J.

2012-09-01

The purpose of CABERNET- Podet-Met (CAmera BEtter Resolution NETwork, Pole sur la Dynamique de l'Environnement Terrestre - Meteor) project is the automated observation, by triangulation with three cameras, of meteor showers to perform a calculation of meteoroids trajectory and velocity. The scientific goal is to search the parent body, comet or asteroid, for each observed meteor. To install outdoor cameras in order to perform astronomy measurements for several years with high reliability requires a very specific design for the box. For these cameras, this contribution shows how we fulfilled the various functions of their boxes, such as cooling of the CCD, heating to melt snow and ice, the protecting against moisture, lightning and Solar light. We present the principal and secondary functions, the product breakdown structure, the technical solutions evaluation grid of criteria, the adopted technology products and their implementation in multifunction subsets for miniaturization purpose. To manage this project, we aim to get the lowest manpower and development time for every part. In appendix, we present the measurements the image quality evolution during the CCD cooling, and some pictures of the prototype.

Dynamics of the presence of israeli acute paralysis virus in honey bee colonies with colony collapse disorder.

PubMed

Hou, Chunsheng; Rivkin, Hadassah; Slabezki, Yossi; Chejanovsky, Nor

2014-05-05

The determinants of Colony Collapse Disorder (CCD), a particular case of collapse of honey bee colonies, are still unresolved. Viruses including the Israeli acute paralysis virus (IAPV) were associated with CCD. We found an apiary with colonies showing typical CCD characteristics that bore high loads of IAPV, recovered some colonies from collapse and tested the hypothesis if IAPV was actively replicating in them and infectious to healthy bees. We found that IAPV was the dominant pathogen and it replicated actively in the colonies: viral titers decreased from April to September and increased from September to December. IAPV extracted from infected bees was highly infectious to healthy pupae: they showed several-fold amplification of the viral genome and synthesis of the virion protein VP3. The health of recovered colonies was seriously compromised. Interestingly, a rise of IAPV genomic copies in two colonies coincided with their subsequent collapse. Our results do not imply IAPV as the cause of CCD but indicate that once acquired and induced to replication it acts as an infectious factor that affects the health of the colonies and may determine their survival. This is the first follow up outside the US of CCD-colonies bearing IAPV under natural conditions.

Dynamics of the Presence of Israeli Acute Paralysis Virus in Honey Bee Colonies with Colony Collapse Disorder

PubMed Central

Hou, Chunsheng; Rivkin, Hadassah; Slabezki, Yossi; Chejanovsky, Nor

2014-01-01

The determinants of Colony Collapse Disorder (CCD), a particular case of collapse of honey bee colonies, are still unresolved. Viruses including the Israeli acute paralysis virus (IAPV) were associated with CCD. We found an apiary with colonies showing typical CCD characteristics that bore high loads of IAPV, recovered some colonies from collapse and tested the hypothesis if IAPV was actively replicating in them and infectious to healthy bees. We found that IAPV was the dominant pathogen and it replicated actively in the colonies: viral titers decreased from April to September and increased from September to December. IAPV extracted from infected bees was highly infectious to healthy pupae: they showed several-fold amplification of the viral genome and synthesis of the virion protein VP3. The health of recovered colonies was seriously compromised. Interestingly, a rise of IAPV genomic copies in two colonies coincided with their subsequent collapse. Our results do not imply IAPV as the cause of CCD but indicate that once acquired and induced to replication it acts as an infectious factor that affects the health of the colonies and may determine their survival. This is the first follow up outside the US of CCD-colonies bearing IAPV under natural conditions. PMID:24800677

Characterization of viral siRNA populations in honey bee colony collapse disorder.

PubMed

Chejanovsky, Nor; Ophir, Ron; Schwager, Michal Sharabi; Slabezki, Yossi; Grossman, Smadar; Cox-Foster, Diana

2014-04-01

Colony Collapse Disorder (CCD), a special case of collapse of honey bee colonies, has resulted in significant losses for beekeepers. CCD-colonies show abundance of pathogens which suggests that they have a weakened immune system. Since honey bee viruses are major players in colony collapse and given the important role of viral RNA interference (RNAi) in combating viral infections we investigated if CCD-colonies elicit an RNAi response. Deep-sequencing analysis of samples from CCD-colonies from US and Israel revealed abundant small interfering RNAs (siRNA) of 21-22 nucleotides perfectly matching the Israeli acute paralysis virus (IAPV), Kashmir virus and Deformed wing virus genomes. Israeli colonies showed high titers of IAPV and a conserved RNAi-pattern of matching the viral genome. That was also observed in sample analysis from colonies experimentally infected with IAPV. Our results suggest that CCD-colonies set out a siRNA response that is specific against predominant viruses associated with colony losses. Copyright © 2014 Elsevier Inc. All rights reserved.

Vilnius Multicolor CCD Photometry of the Open Cluster NGC 752

NASA Astrophysics Data System (ADS)

Bartašiūtė, S.; Janusz, R.; Boyle, R. P.; Philip, A. G. Davis

We have performed multicolor CCD observations of the central area of NGC 752 to search for faint, low-mass members of this open cluster. Four 12'x12' fields were taken on the 1.8 m Vatican Advanced Technology Telescope (Mt. Graham, Arizona) using a 4K CCD camera and eight intermediate-band filters of the Strömvil system. In this paper we present a catalog of photometry for 405 stars down to the limiting magnitude V=18.5, which contains V magnitudes and color indices of the Vilnius system, together with photometric determinations of spectral types, absolute magnitudes MV, interstellar reddening values EY-V and metallicity parameters [Fe/H]. The good quality multicolor data made it possible to identify the locus of the lower main sequence to four magnitudes beyond the previous (photographic) limit. A relatively small number of photometric members identified at faint magnitudes seems to be indicative of actual dissolution of the cluster from the low-mass end.

Note: A simple multi-channel optical system for modulation spectroscopies.

PubMed

Solís-Macías, J; Sánchez-López, J D; Castro-García, R; Flores-Camacho, J M; Flores-Rangel, G; Ciou, Jian-Jhih; Chen, Kai-Wei; Chen, Chang-Hsiao; Lastras-Martínez, L F; Balderas-Navarro, R E

2017-12-01

Photoreflectance-difference (PR/PRD) and reflectance-difference (RD) spectroscopies employ synchronic detection usually with lock-in amplifiers operating at moderate (200-1000 Hz) and high (50-100 KHz) modulation frequencies, respectively. Here, we report a measurement system for these spectroscopies based on a multichannel CCD spectrometer without a lock-in amplifier. In the proposed scheme, a typical PRD or RD spectrum consists of numerical subtractions between a thousand CCD captures recorded, while a photoelastic modulator is either operating or inhibited. This is advantageous and fits the slow response of CCD detectors to high modulation frequencies. The resulting spectra are processed with Savitzky-Golay filtering and compared well with those measured with conventional scanning systems based on lock-in amplifiers.

Active pixel sensors: the sensor of choice for future space applications?

NASA Astrophysics Data System (ADS)

Leijtens, Johan; Theuwissen, Albert; Rao, Padmakumar R.; Wang, Xinyang; Xie, Ning

2007-10-01

It is generally known that active pixel sensors (APS) have a number of advantages over CCD detectors if it comes to cost for mass production, power consumption and ease of integration. Nevertheless, most space applications still use CCD detectors because they tend to give better performance and have a successful heritage. To this respect a change may be at hand with the advent of deep sub-micron processed APS imagers (< 0.25-micron feature size). Measurements performed on test structures at the University of Delft have shown that the imagers are very radiation tolerant even if made in a standard process without the use of special design rules. Furthermore it was shown that the 1/f noise associated with deep sub-micron imagers is reduced as compared to previous generations APS imagers due to the improved quality of the gate oxides. Considering that end of life performance will have to be guaranteed, limited budget for adding shielding metal will be available for most applications and lower power operations is always seen as a positive characteristic in space applications, deep sub-micron APS imagers seem to have a number of advantages over CCD's that will probably cause them to replace CCD's in those applications where radiation tolerance and low power operation are important

Tests of commercial colour CMOS cameras for astronomical applications

NASA Astrophysics Data System (ADS)

Pokhvala, S. M.; Reshetnyk, V. M.; Zhilyaev, B. E.

2013-12-01

We present some results of testing commercial colour CMOS cameras for astronomical applications. Colour CMOS sensors allow to perform photometry in three filters simultaneously that gives a great advantage compared with monochrome CCD detectors. The Bayer BGR colour system realized in colour CMOS sensors is close to the astronomical Johnson BVR system. The basic camera characteristics: read noise (e^{-}/pix), thermal noise (e^{-}/pix/sec) and electronic gain (e^{-}/ADU) for the commercial digital camera Canon 5D MarkIII are presented. We give the same characteristics for the scientific high performance cooled CCD camera system ALTA E47. Comparing results for tests of Canon 5D MarkIII and CCD ALTA E47 show that present-day commercial colour CMOS cameras can seriously compete with the scientific CCD cameras in deep astronomical imaging.

Signal detectability in diffusive media using phased arrays in conjunction with detector arrays.

PubMed

Kang, Dongyel; Kupinski, Matthew A

2011-06-20

We investigate Hotelling observer performance (i.e., signal detectability) of a phased array system for tasks of detecting small inhomogeneities and distinguishing adjacent abnormalities in uniform diffusive media. Unlike conventional phased array systems where a single detector is located on the interface between two sources, we consider a detector array, such as a CCD, on a phantom exit surface for calculating the Hotelling observer detectability. The signal detectability for adjacent small abnormalities (2 mm displacement) for the CCD-based phased array is related to the resolution of reconstructed images. Simulations show that acquiring high-dimensional data from a detector array in a phased array system dramatically improves the detectability for both tasks when compared to conventional single detector measurements, especially at low modulation frequencies. It is also observed in all studied cases that there exists the modulation frequency optimizing CCD-based phased array systems, where detectability for both tasks is consistently high. These results imply that the CCD-based phased array has the potential to achieve high resolution and signal detectability in tomographic diffusive imaging while operating at a very low modulation frequency. The effect of other configuration parameters, such as a detector pixel size, on the observer performance is also discussed.

A High Resolution TDI CCD Camera forMicrosatellite (HRCM)

NASA Astrophysics Data System (ADS)

Hao, Yuncai; Zheng, You; Dong, Ying; Li, Tao; Yu, Shijie

In resent years it is a important development direction in the commercial remote sensing field to obtain (1-5)m high ground resolution from space using microsatellite. Thanks to progress of new technologies, new materials and new detectors it is possible to develop 1m ground resolution space imaging system with weight less than 20kg. Based on many years works on optical system design a project of very high resolution TDI CCD camera using in space was proposed by the authors of this paper. The performance parameters and optical lay-out of the HRCM was presented. A compact optical design and results analysis for the system was given in the paper also. and small fold mirror to take a line field of view usable for TDI CCD and short outer size. The length along the largest size direction is about 1/4 of the focal length. And two 4096X96(grades) line TDI CCD will be used as the focal plane detector. The special optical parts are fixed near before the final image for getting the ground pixel resolution higher than the Nyquist resolution of the detector using the sub-pixel technique which will be explained in the paper. In the system optical SiC will be used as the mirror material, the C-C composite material will be used as the material of the mechanical structure framework. The circle frame of the primary and secondary mirrors will use one time turning on a machine tool in order to assuring concentric request for alignment of the system. In general the HRCM have the performance parameters with 2.5m focal length, 20 FOV, 1/11relative aperture, (0.4-0.8) micrometer spectral range, 10 micron pixel size of TDI CCD, weight less than 20kg, 1m ground pixel resolution at flying orbit 500km high. Design and analysis of the HRCM put up in the paper indicate that HRCM have many advantages to use it in space. Keywords High resolution TDI CCD Sub-pixel imaging Light-weighted optical system SiC mirror

A configurable distributed high-performance computing framework for satellite's TDI-CCD imaging simulation

NASA Astrophysics Data System (ADS)

Xue, Bo; Mao, Bingjing; Chen, Xiaomei; Ni, Guoqiang

2010-11-01

This paper renders a configurable distributed high performance computing(HPC) framework for TDI-CCD imaging simulation. It uses strategy pattern to adapt multi-algorithms. Thus, this framework help to decrease the simulation time with low expense. Imaging simulation for TDI-CCD mounted on satellite contains four processes: 1) atmosphere leads degradation, 2) optical system leads degradation, 3) electronic system of TDI-CCD leads degradation and re-sampling process, 4) data integration. Process 1) to 3) utilize diversity data-intensity algorithms such as FFT, convolution and LaGrange Interpol etc., which requires powerful CPU. Even uses Intel Xeon X5550 processor, regular series process method takes more than 30 hours for a simulation whose result image size is 1500 * 1462. With literature study, there isn't any mature distributing HPC framework in this field. Here we developed a distribute computing framework for TDI-CCD imaging simulation, which is based on WCF[1], uses Client/Server (C/S) layer and invokes the free CPU resources in LAN. The server pushes the process 1) to 3) tasks to those free computing capacity. Ultimately we rendered the HPC in low cost. In the computing experiment with 4 symmetric nodes and 1 server , this framework reduced about 74% simulation time. Adding more asymmetric nodes to the computing network, the time decreased namely. In conclusion, this framework could provide unlimited computation capacity in condition that the network and task management server are affordable. And this is the brand new HPC solution for TDI-CCD imaging simulation and similar applications.

Turbulent Mixing and Combustion for High-Speed Air-Breathing Propulsion Application

DTIC Science & Technology

2007-08-12

deficit (the velocity of the wake relative to the free-stream velocity), decays rapidly with downstream distance, so that the streamwise velocity is...switched laser with double-pulse option) and a new imaging system (high-resolution: 4008x2672 pix2, low- noise (cooled) Cooke PCO-4000 CCD camera). The...was designed in-house for high-speed low- noise image acquisition. The KFS CCD image sensor was designed by Mark Wadsworth of JPL and has a resolution

Does topical wound oxygen (TWO2) offer an improved outcome over conventional compression dressings (CCD) in the management of refractory venous ulcers (RVU)? A parallel observational comparative study.

PubMed

Tawfick, W; Sultan, S

2009-07-01

Topical wound oxygen (TWO(2)) may help wound healing in the management of refractory venous ulcers (RVU). The aim of this study was to measure the effect of TWO(2) on wound healing using the primary end-point of the proportion of ulcers healed at 12 weeks. Secondary end-points were time to full healing, percentage of reduction in ulcer size, pain reduction, recurrence rates and Quality-Adjusted Time Spent Without Symptoms of disease and Toxicity of Treatment (Q-TWiST). A parallel observational comparative study. Patients with CEAP C(6,s) RVU, assessed by duplex ultrasonography, were managed with either TWO(2) (n=46) or conventional compression dressings (CCD) (n=37) for 12 weeks or till full healing. Patients were followed up at 3 monthly intervals. At 12 weeks, 80% of TWO(2) managed ulcers were completely healed, compared to 35% of CCD ulcers (p<0.0001). Median time to full healing was 45 days in TWO(2) patients and 182 days in CCD patients (p<0.0001). The pain score threshold in TWO(2) managed patients improved from 8 to 3 by 13 days. After 12-month follow-up, 5 of the 13 healed CCD ulcers showed signs of recurrence compared to none of the 37 TWO(2) healed ulcers. TWO(2) patients experienced a significantly improved Q-TWiST. TWO(2) reduces recurrence rates, alleviates pain and improves the Q-TWiST. We believe it is a valuable tool in the armamentarium of management of RVU.

Development and performances of very long stripe-filters for a multispectral detector

NASA Astrophysics Data System (ADS)

Laubier, David; Gimenez, Thierry; Mercier-Ythier, Renaud

2002-01-01

The development in the past few years of all-mirror telescopes has opened a wide new range of possibilities to instrument designers, with features like high compactness and outstanding optical quality over wide fields of view. However, this design imposes specific constraints on the focal plane: it can no longer accommodate glass beamsplitters and its size increases with the field of view. New CCD detectors with multiple long lines are well-suited to this application, but require a new filters strategy. This paper will detail what ours was in the particular case of a 78-mm long, 4-channel CCD. The choice of the stripe- filters concept was made on the basis of a performance versus cost analysis. Two kinds of assemblies were retained at this stage. The components manufactured by SAGEM-REOSC PRODUCTS in an initial development phase showed good spectral performance with high rejection over a very wide range of wavelengths. Some topics like local defects and straylight needed specific work. The paper focuses on the impact of the defects on the performances and the way they have been dealt with, and on the straylight design strategy with the results obtained in the different cases. In particular, it shows how the detector's design can be partially driven by the straylight requirements.

Measuring high-resolution sky luminance distributions with a CCD camera.

PubMed

Tohsing, Korntip; Schrempf, Michael; Riechelmann, Stefan; Schilke, Holger; Seckmeyer, Gunther

2013-03-10

We describe how sky luminance can be derived from a newly developed hemispherical sky imager (HSI) system. The system contains a commercial compact charge coupled device (CCD) camera equipped with a fish-eye lens. The projection of the camera system has been found to be nearly equidistant. The luminance from the high dynamic range images has been calculated and then validated with luminance data measured by a CCD array spectroradiometer. The deviation between both datasets is less than 10% for cloudless and completely overcast skies, and differs by no more than 20% for all sky conditions. The global illuminance derived from the HSI pictures deviates by less than 5% and 20% under cloudless and cloudy skies for solar zenith angles less than 80°, respectively. This system is therefore capable of measuring sky luminance with the high spatial and temporal resolution of more than a million pixels and every 20 s respectively.

Congenital chloride diarrhea misdiagnosed as pseudo-Bartter syndrome.

PubMed

Saneian, Hossein; Bahraminia, Emad

2013-09-01

Congenital chloride diarrhea (CCD) is a rare autosomal recessive disease which is characterized by intractable diarrhea of infancy, failure to thrive, high fecal chloride, hypochloremia, hypokalemia, hyponatremia and metabolic alkalosis. In this case report, we present the first female and the second official case of CCD in Iran. A 15-month-old girl referred to our hospital due to failure to thrive and poor feeding. She had normal kidneys, liver and spleen. Treating her with Shohl's solution, thiazide and zinc sulfate did not result in weight gain. Consequently, pseudo-Bartter syndrome was suspected, she was treated with intravenous (IV) therapy to which she responded dramatically. In addition, hypokalemia resolved quickly. Since this does not usually happen in patients with the pseudo-Bartter syndrome, stool tests were performed. Abnormal level of chloride in stool suggested CCD and she was thus treated with IV fluid replacement, Total parentral nutrition and high dose of oral omeprazole (3 mg/kg/day). She gained 1 kg of weight and is doing fine until present. CCD is a rare hereditary cause of intractable diarrhea of infancy. It should be considered in infants with unknown severe electrolyte disturbances.

New-style defect inspection system of film

NASA Astrophysics Data System (ADS)

Liang, Yan; Liu, Wenyao; Liu, Ming; Lee, Ronggang

2002-09-01

An inspection system has been developed for on-line detection of film defects, which bases on combination of photoelectric imaging and digital image processing. The system runs in high speed of maximum 60m/min. Moving film is illuminated by LED array which emits even infrared (peak wavelength λp=940nm), and infrared images are obtained with a high quality and high speed CCD camera. The application software based on Visual C++6.0 under Windows processes images in real time by means of such algorithms as median filter, edge detection and projection, etc. The system is made up of four modules, which are introduced in detail in the paper. On-line experiment results shows that the inspection system can recognize defects precisely in high speed and run reliably in practical application.

Application of the CCD Fabry-Perot Annular Summing Technique to Thermospheric O(1)D.

NASA Astrophysics Data System (ADS)

Coakley, Monica Marie

1995-01-01

This work will detail the verification of the advantages of the Fabry-Perot charge coupled device (CCD) annular summing technique, the development of the technique for analysis of daysky spectra, and the implications of the resulting spectra for neutral temperature and wind measurements in the daysky thermosphere. The daysky spectral feature of interest is the bright (1 kilo-Rayleigh) thermospheric (OI) emission at 6300 A which had been observed in the nightsky in order to determine winds and temperatures in the vicinity of the altitude of 250 km. In the daysky, the emission line sits on top of a bright Rayleigh scattered continuum background which significantly complicates the observation. With a triple etalon Fabry-Perot spectrometer, the continuum background can be reduced while maintaining high throughput and high resolution. The inclusion of a CCD camera results in significant savings in integration time over the two more standard scanning photomultiplier systems that have made the same wind and temperature measurements in the past. A comparable CCD system can experience an order of magnitude savings in integration time over a PMT system. Laboratory and field tests which address the advantages and limitations of both the Fabry-Perot CCD annular summing technique and the daysky CCD imaging are included in Chap. 2 and Chap. 3. With a sufficiently large throughput associated with the spectrometer and a CCD detector, rapid observations (~4 minute integrations) can be made. Extraction of the line width and line center from the daysky near-continuum background is complicated compared to the nightsky case, but possible. Methods of fitting the line are included in Chap. 4. The daysky O ^1D temperatures are consistent with a lower average emission height than predicted by models. The data and models are discussed in Chap. 5. Although some discrepancies exist between resulting temperatures and models, the observations indicate the potential for other direct measurements of bright neutral species in the daysky as well as the potential for twenty-four hour coverage.

Performance of the upgraded LTP-II at the ALS Optical Metrology Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Advanced Light Source; Yashchuk, Valeriy V; Kirschman, Jonathan L.

2008-07-14

The next generation of synchrotrons and free electron laser facilities requires x-ray optical systems with extremely high performance, generally of diffraction limited quality. Fabrication and use of such optics requires adequate, highly accurate metrology and dedicated instrumentation. Previously, we suggested ways to improve the performance of the Long Trace Profiler (LTP), a slope measuring instrument widely used to characterize x-ray optics at long spatial wavelengths. The main way is use of a CCD detector and corresponding technique for calibration of photo-response non-uniformity [J. L. Kirschman, et al., Proceedings of SPIE 6704, 67040J (2007)]. The present work focuses on the performancemore » and characteristics of the upgraded LTP-II at the ALS Optical Metrology Laboratory. This includes a review of the overall aspects of the design, control system, the movement and measurement regimes for the stage, and analysis of the performance by a slope measurement of a highly curved super-quality substrate with less than 0.3 microradian (rms)slope variation.« less

A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments

PubMed Central

Rodrigo, María J.; Alquézar, Berta; Al-Babili, Salim

2013-01-01

Citrus is the first tree crop in terms of fruit production. The colour of Citrus fruit is one of the main quality attributes, caused by the accumulation of carotenoids and their derivative C30 apocarotenoids, mainly β-citraurin (3-hydroxy-β-apo-8′-carotenal), which provide an attractive orange-reddish tint to the peel of oranges and mandarins. Though carotenoid biosynthesis and its regulation have been extensively studied in Citrus fruits, little is known about the formation of C30 apocarotenoids. The aim of this study was to the identify carotenoid cleavage enzyme(s) [CCD(s)] involved in the peel-specific C30 apocarotenoids. In silico data mining revealed a new family of five CCD4-type genes in Citrus. One gene of this family, CCD4b1, was expressed in reproductive and vegetative tissues of different Citrus species in a pattern correlating with the accumulation of C30 apocarotenoids. Moreover, developmental processes and treatments which alter Citrus fruit peel pigmentation led to changes of β-citraurin content and CCD4b1 transcript levels. These results point to the involvement of CCD4b1 in β-citraurin formation and indicate that the accumulation of this compound is determined by the availability of the presumed precursors zeaxanthin and β-cryptoxanthin. Functional analysis of CCD4b1 by in vitro assays unequivocally demonstrated the asymmetric cleavage activity at the 7′,8′ double bond in zeaxanthin and β-cryptoxanthin, confirming its role in C30 apocarotenoid biosynthesis. Thus, a novel plant carotenoid cleavage activity targeting the 7′,8′ double bond of cyclic C40 carotenoids has been identified. These results suggest that the presented enzyme is responsible for the biosynthesis of C30 apocarotenoids in Citrus which are key pigments in fruit coloration. PMID:24006419

Cross-reacting carbohydrate determinants and hymenoptera venom allergy.

PubMed

Brehler, Randolf; Grundmann, Sonja; Stöcker, Benedikt

2013-08-01

Insect venom allergy is an important cause of anaphylaxis. Venom immunotherapy assume the clear identification of the culprit insect, but this is impeded by Immunoglobulin E (IgE) antibodies to cross reactive carbohydrate determinant (CCD) epitopes of common glycoproteins. Here we give an overview about inducers, importance, and relevance of anti-N-Glycan CCD IgE antibodies. Pollen exposure and insect stings induce anti-CCD IgE antibodies interfering with in-vitro tests for allergy diagnosis due to extensive IgE cross-reactivity. Instead of being biologically active these antibodies are irrelevant for allergic reactions due to hymenoptera stings. The general response of the immune system to the ubiquitous exposure to N-glycan containing glycoproteins is still a matter of debate. CCD specific IgG antibodies in sera of bee keepers suggest tolerance induction due to high-dose exposure. Tolerance induction by pollen and food glycoproteins has not been proved. Hymenoptera stings and pollen exposure induce anti-CCD IgE. In regard to anaphylaxis due to Hymenoptera stings these antibodies are not clinically relevant, but they are important for the specificity of in-vitro tests proving insect venom allergy. The introduction of component based diagnostic IgE testing improves the specificity of in-vitro tests if proteins devoid of CCD epitopes are used.

Design principles and applications of a cooled CCD camera for electron microscopy.

PubMed

Faruqi, A R

1998-01-01

Cooled CCD cameras offer a number of advantages in recording electron microscope images with CCDs rather than film which include: immediate availability of the image in a digital format suitable for further computer processing, high dynamic range, excellent linearity and a high detective quantum efficiency for recording electrons. In one important respect however, film has superior properties: the spatial resolution of CCD detectors tested so far (in terms of point spread function or modulation transfer function) are inferior to film and a great deal of our effort has been spent in designing detectors with improved spatial resolution. Various instrumental contributions to spatial resolution have been analysed and in this paper we discuss the contribution of the phosphor-fibre optics system in this measurement. We have evaluated the performance of a number of detector components and parameters, e.g. different phosphors (and a scintillator), optical coupling with lens or fibre optics with various demagnification factors, to improve the detector performance. The camera described in this paper, which is based on this analysis, uses a tapered fibre optics coupling between the phosphor and the CCD and is installed on a Philips CM12 electron microscope equipped to perform cryo-microscopy. The main use of the camera so far has been in recording electron diffraction patterns from two dimensional crystals of bacteriorhodopsin--from wild type and from different trapped states during the photocycle. As one example of the type of data obtained with the CCD camera a two dimensional Fourier projection map from the trapped O-state is also included. With faster computers, it will soon be possible to undertake this type of work on an on-line basis. Also, with improvements in detector size and resolution, CCD detectors, already ideal for diffraction, will be able to compete with film in the recording of high resolution images.

Development of a real-time digital radiography system using a scintillator-type flat-panel detector

NASA Astrophysics Data System (ADS)

Ikeda, Shigeyuki; Suzuki, Katsumi; Ishikawa, Ken; Okajima, Kenichi

2001-06-01

In order to study the advantage and remaining problems of FPD (flat panel detector) for clinical use by the real-time DR (digital radiography) system, we developed a prototype system using a scintillator type FPD and which was compared with previous I.I.-CCD type real-time DR. We replaced the X- ray detector of DR-2000X from I.I.-4M (4 million pixels)-CCD camera to the scintillator type dynamic FPD(7' X 9', 127 micrometers ), which can take both radiographic and fluoroscopic images. We obtained the images of head and stomach phantoms, and discussed about the image quality with medical doctors.

Follow-up study of children with cerebral coordination disturbance (CCD, Vojta).

PubMed

Imamura, S; Sakuma, K; Takahashi, T

1983-01-01

713 children (from newborn to 12-month-old) with delayed motor development were carefully examined and classified into normal, very light cerebral coordination disturbance (CCD, Vojta), light CCD, moderate CCD, severe CCD, suspected cerebral palsy (CP) and other diseases at their first visit, and were followed up carefully. Finally, 89.0% of very light CCD, 71.4% of light CCD, 56.0% of moderate CCD and 30.0% of severe CCD developed into normal. 59.5% of moderate CCD and 45.5% of severe CCD among children who were given Vojta's physiotherapy developed into normal. The classification of cases with delayed motor development into very light, light, moderate and severe CCD based on the extent of abnormality in their postural reflexes is useful and well correlated with their prognosis. Treatment by Vojta's method seems to be efficient and helpful for young children with delayed motor development.

Quantum efficiency measurement of the Transiting Exoplanet Survey Satellite (TESS) CCD detectors

NASA Astrophysics Data System (ADS)

Krishnamurthy, A.; Villasenor, J.; Thayer, C.; Kissel, S.; Ricker, G.; Seager, S.; Lyle, R.; Deline, A.; Morgan, E.; Sauerwein, T.; Vanderspek, R.

2016-07-01

Very precise on-ground characterization and calibration of TESS CCD detectors will significantly assist in the analysis of the science data from the mission. An accurate optical test bench with very high photometric stability has been developed to perform precise measurements of the absolute quantum efficiency. The setup consists of a vacuum dewar with a single MIT Lincoln Lab CCID-80 device mounted on a cold plate with the calibrated reference photodiode mounted next to the CCD. A very stable laser-driven light source is integrated with a closed-loop intensity stabilization unit to control variations of the light source down to a few parts-per-million when averaged over 60 s. Light from the stabilization unit enters a 20 inch integrating sphere. The output light from the sphere produces near-uniform illumination on the cold CCD and on the calibrated reference photodiode inside the dewar. The ratio of the CCD and photodiode signals provides the absolute quantum efficiency measurement. The design, key features, error analysis, and results from the test campaign are presented.

MISR Level 1A CCD, 1B1, 1B2, and Browse Products

Atmospheric Science Data Center

2013-04-01

... ESDT Product File Name Prefix Current Quality Designations MI1B2E MISR_AM1_GRP_ELLIPSOID_GM, ... should be used. All calibration files for the life of the mission have been reprocessed using the best available calibration. ...

Sonnenaufnahmen mit einer Starlight X-Press CCD-Kamera.

NASA Astrophysics Data System (ADS)

Bernhard, K.

1997-03-01

To take-up the sun, most amateurastronomers use the photographic method. In this article the author shows, that CCD is also a suitable method to do this. Especially the possibilities to see immediately the result of focusing on the screen and the electronic processing are very useful to get sharp and high-contrast images of the sun.

Design Method For Ultra-High Resolution Linear CCD Imagers

NASA Astrophysics Data System (ADS)

Sheu, Larry S.; Truong, Thanh; Yuzuki, Larry; Elhatem, Abdul; Kadekodi, Narayan

1984-11-01

This paper presents the design method to achieve ultra-high resolution linear imagers. This method utilizes advanced design rules and novel staggered bilinear photo sensor arrays with quadrilinear shift registers. Design constraint in the detector arrays and shift registers are analyzed. Imager architecture to achieve ultra-high resolution is presented. The characteristics of MTF, aliasing, speed, transfer efficiency and fine photolithography requirements associated with this architecture are also discussed. A CCD imager with advanced 1.5 um minimum feature size was fabricated. It is intended as a test vehicle for the next generation small sampling pitch ultra-high resolution CCD imager. Standard double-poly, two-phase shift registers were fabricated at an 8 um pitch using the advanced design rules. A special process step that blocked the source-drain implant from the shift register area was invented. This guaranteed excellent performance of the shift registers regardless of the small poly overlaps. A charge transfer efficiency of better than 0.99995 and maximum transfer speed of 8 MHz were achieved. The imager showed excellent performance. The dark current was less than 0.2 mV/ms, saturation 250 mV, adjacent photoresponse non-uniformity ± 4% and responsivity 0.7 V/ μJ/cm2 for the 8 μm x 6 μm photosensor size. The MTF was 0.6 at 62.5 cycles/mm. These results confirm the feasibility of the next generation ultra-high resolution CCD imagers.

Active Pixel Sensors: Are CCD's Dinosaurs?

NASA Technical Reports Server (NTRS)

Fossum, Eric R.

1993-01-01

Charge-coupled devices (CCD's) are presently the technology of choice for most imaging applications. In the 23 years since their invention in 1970, they have evolved to a sophisticated level of performance. However, as with all technologies, we can be certain that they will be supplanted someday. In this paper, the Active Pixel Sensor (APS) technology is explored as a possible successor to the CCD. An active pixel is defined as a detector array technology that has at least one active transistor within the pixel unit cell. The APS eliminates the need for nearly perfect charge transfer -- the Achilles' heel of CCDs. This perfect charge transfer makes CCD's radiation 'soft,' difficult to use under low light conditions, difficult to manufacture in large array sizes, difficult to integrate with on-chip electronics, difficult to use at low temperatures, difficult to use at high frame rates, and difficult to manufacture in non-silicon materials that extend wavelength response.

Image Improvement Techniques

NASA Astrophysics Data System (ADS)

Shine, R. A.

1997-05-01

Over the last decade, a repertoire of techniques have been developed and/or refined to improve the quality of high spatial resolution solar movies taken from ground based observatories. These include real time image motion corrections, frame selection, phase diversity measurements of the wavefront, and extensive post processing to partially remove atmospheric distortion. Their practical application has been made possible by the increasing availability and decreasing cost of large CCD's with fast digital readouts and high speed computer workstations with large memories. Most successful have been broad band (0.3 to 10 nm) filtergram movies which can use exposure times of 10 to 30 ms, short enough to ``freeze'' atmospheric motions. Even so, only a handful of movies with excellent image quality for more than a hour have been obtained to date. Narrowband filtergrams (about 0.01 nm), such as those required for constructing magnetograms and Dopplergrams, have been more challenging although some single images approach the quality of the best continuum images. Some promising new techniques and instruments, together with persistence and good luck, should continue the progress made in the last several years.

Test technology on divergence angle of laser range finder based on CCD imaging fusion

NASA Astrophysics Data System (ADS)

Shi, Sheng-bing; Chen, Zhen-xing; Lv, Yao

2016-09-01

Laser range finder has been equipped with all kinds of weapons, such as tank, ship, plane and so on, is important component of fire control system. Divergence angle is important performance and incarnation of horizontal resolving power for laser range finder, is necessary appraised test item in appraisal test. In this paper, based on high accuracy test on divergence angle of laser range finder, divergence angle test system is designed based on CCD imaging, divergence angle of laser range finder is acquired through fusion technology for different attenuation imaging, problem that CCD characteristic influences divergence angle test is solved.

Producing CCD imaging sensor with flashed backside metal film

NASA Technical Reports Server (NTRS)

Janesick, James R. (Inventor)

1988-01-01

A backside illuminated CCD imaging sensor for reading out image charges from wells of the array of pixels is significantly improved for blue, UV, far UV and low energy x-ray wavelengths (1-5000.ANG.) by so overthinning the backside as to place the depletion edge at the surface and depositing a thin transparent metal film of about 10.ANG. on a native-quality oxide film of less than about 30.ANG. grown on the thinned backside. The metal is selected to have a higher work function than that of the semiconductor to so bend the energy bands (at the interface of the semiconductor material and the oxide film) as to eliminate wells that would otherwise trap minority carriers. A bias voltage may be applied to extend the frontside depletion edge to the interface of the semiconductor material with the oxide film in the event there is not sufficient thinning. This metal film (flash gate), which improves and stabilizes the quantum efficiency of a CCD imaging sensor, will also improve the QE of any p-n junction photodetector.

CCD imaging sensor with flashed backside metal film

NASA Technical Reports Server (NTRS)

Janesick, James R. (Inventor)

1991-01-01

A backside illuminated CCD imaging sensor for reading out image charges from wells of the array of pixels is significantly improved for blue, UV, far UV and low energy x-ray wavelengths (1-5000.ANG.) by so overthinning the backside as to place the depletion edge at the surface and depositing a thin transparent metal film of about 10.ANG. on a native-quality oxide film of less than about 30.ANG. grown on the thinned backside. The metal is selected to have a higher work function than that of the semiconductor to so bend the energy bands (at the interface of the semiconductor material and the oxide film) as to eliminate wells that would otherwise trap minority carriers. A bias voltage may be applied to extend the frontside depletion edge to the interface of the semiconductor material with the oxide film in the event there is not sufficient thinning. This metal film (flash gate), which improves and stabilizes the quantum efficiency of a CCD imaging sensor, will also improve the QE of any p-n junction photodetector.

Initial results from a cryogenic proton irradiation of a p-channel CCD

NASA Astrophysics Data System (ADS)

Gow, J. P. D.; Wood, D.; Burt, D.; Hall, D. J.; Dryer, B.; Holland, A. D.; Murray, N. J.

2015-08-01

The displacement damage hardness that can be achieved using p-channel charge coupled devices (CCD) was originally demonstrated in 1997 and since then a number of other studies have demonstrated an improved tolerance to radiationinduced CTI when compared to n-channel CCDs. A number of recent studies have also shown that the temperature history of the device after the irradiation impacts the performance of the detector, linked to the mobility of defects at different temperatures. This study describes the initial results from an e2v technologies p-channel CCD204 irradiated at 153 K with a 10 MeV equivalent proton fluences of 1.24×109 and 1.24×1011 protons.cm-2. The number of defects identified using trap pumping, dark current and cosmetic quality immediately after irradiation and over a period of 150 hours after the irradiation with the device held at 153 K and then after different periods of time at room temperature are described. The device also exhibited a flatband voltage shift of around 30 mV per krad, determined by the reduction in full well capacity.

Characterization of a fully depleted CCD on high-resistivity silicon

NASA Astrophysics Data System (ADS)

Stover, Richard J.; Wei, Mingzhi; Lee, Y.; Gilmore, David K.; Holland, S. E.; Groom, D. E.; Moses, William W.; Perlmutter, Saul; Goldhaber, G.; Pennypacker, C.; Wang, N. W.; Palaio, N.

1997-04-01

Most scientific CCD imagers are fabricated on 30-50 (Omega) - cm epitaxial silicon. When illuminated form the front side of the device they generally have low quantum efficiency in the blue region of the visible spectrum because of strong absorption in the polycrystalline silicon gates as well as poor quantum efficiency in the far red and near infrared region of the spectrum because of the shallow depletion depth of the low-resistivity silicon. To enhance the blue response of scientific CCDs they are often thinned and illuminated from the back side. While blue response is greatly enhanced by this process, it is expensive and it introduces additional problems for the red end of the spectrum. A typical thinned CCD is 15 to 25 micrometers thick, and at wavelengths beyond about 800 nm the absorption depth becomes comparable to the thickness of the device, leading to interference fringes from reflected light. Because these interference fringes are of high order, the spatial pattern of the fringes is extremely sensitive to small changes in the optical illumination of the detector. Calibration and removal of the effects of the fringes is one of the primary limitations on the performance of astronomical images taken at wavelengths of 800 nm or more. In this paper we present results from the characterization of a CCD which promises to address many of the problems of typical thinned CCDs. The CCD reported on here was fabricated at Lawrence Berkeley National Laboratory (LBNL) on a 10-12 K$OMega-cm n-type silicon substrate.THe CCD is a 200 by 200 15-micrometers square pixel array, and due to the very high resistivity of the starting material, the entire 300 micrometers substrate is depleted. Full depletion works because of the gettering technology developed at LBNL which keeps leakage current down. Both front-side illuminated and backside illuminated devices have been tested. We have measured quantum efficiency, read-noise, full-well, charge-transfer efficiency, and leakage current. We have also observed the effects of clocking waveform shapes on spurious charge generation. While these new CCDs promise to be a major advance in CD technology, they too have limitations such as charge spreading and cosmic-ray effects. These limitations have been characterized and are presented. Examples of astronomical observations obtained with the backside CCD on the 1-meter reflector at Lick Observatory are presented.

How do I convert the numbers stored in the files to physical quantities?

Atmospheric Science Data Center

2014-12-08

At Level 1A, the 14 most significant bits (MSB) directly represent the raw digital count from the camera's Charge-Coupled Device (CCD). The 2 least significant bits (LSB) of the 16-bit data values are data quality indicators (DQI). A...

High-resolution, high-sensitivity, ground-based solar spectropolarimetry with a new fast imaging polarimeter. I. Prototype characterization

NASA Astrophysics Data System (ADS)

Iglesias, F. A.; Feller, A.; Nagaraju, K.; Solanki, S. K.

2016-05-01

Context. Remote sensing of weak and small-scale solar magnetic fields is of utmost relevance when attempting to respond to a number of important open questions in solar physics. This requires the acquisition of spectropolarimetric data with high spatial resolution (~10-1 arcsec) and low noise (10-3 to 10-5 of the continuum intensity). The main limitations to obtain these measurements from the ground, are the degradation of the image resolution produced by atmospheric seeing and the seeing-induced crosstalk (SIC). Aims: We introduce the prototype of the Fast Solar Polarimeter (FSP), a new ground-based, high-cadence polarimeter that tackles the above-mentioned limitations by producing data that are optimally suited for the application of post-facto image restoration, and by operating at a modulation frequency of 100 Hz to reduce SIC. Methods: We describe the instrument in depth, including the fast pnCCD camera employed, the achromatic modulator package, the main calibration steps, the effects of the modulation frequency on the levels of seeing-induced spurious signals, and the effect of the camera properties on the image restoration quality. Results: The pnCCD camera reaches 400 fps while keeping a high duty cycle (98.6%) and very low noise (4.94 e- rms). The modulator is optimized to have high (>80%) total polarimetric efficiency in the visible spectral range. This allows FSP to acquire 100 photon-noise-limited, full-Stokes measurements per second. We found that the seeing induced signals that are present in narrow-band, non-modulated, quiet-sun measurements are (a) lower than the noise (7 × 10-5) after integrating 7.66 min, (b) lower than the noise (2.3 × 10-4) after integrating 1.16 min and (c) slightly above the noise (4 × 10-3) after restoring case (b) by means of a multi-object multi-frame blind deconvolution. In addition, we demonstrate that by using only narrow-band images (with low S/N of 13.9) of an active region, we can obtain one complete set of high-quality restored measurements about every 2 s.

LED characterization for development of on-board calibration unit of CCD-based advanced wide-field sensor camera of Resourcesat-2A

NASA Astrophysics Data System (ADS)

Chatterjee, Abhijit; Verma, Anurag

2016-05-01

The Advanced Wide Field Sensor (AWiFS) camera caters to high temporal resolution requirement of Resourcesat-2A mission with repeativity of 5 days. The AWiFS camera consists of four spectral bands, three in the visible and near IR and one in the short wave infrared. The imaging concept in VNIR bands is based on push broom scanning that uses linear array silicon charge coupled device (CCD) based Focal Plane Array (FPA). On-Board Calibration unit for these CCD based FPAs is used to monitor any degradation in FPA during entire mission life. Four LEDs are operated in constant current mode and 16 different light intensity levels are generated by electronically changing exposure of CCD throughout the calibration cycle. This paper describes experimental setup and characterization results of various flight model visible LEDs (λP=650nm) for development of On-Board Calibration unit of Advanced Wide Field Sensor (AWiFS) camera of RESOURCESAT-2A. Various LED configurations have been studied to meet dynamic range coverage of 6000 pixels silicon CCD based focal plane array from 20% to 60% of saturation during night pass of the satellite to identify degradation of detector elements. The paper also explains comparison of simulation and experimental results of CCD output profile at different LED combinations in constant current mode.

A simple, low-cost, versatile CCD spectrometer for plasma spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Den Hartog, D. J.; Holly, D. J.

1996-06-01

The authors have constructed a simple, low-cost CCD spectrometer capable of both high resolution ({Delta}{lambda} {le} 0.015 nm) and large bandpass (110 nm with {Delta}{lambda} {approximately}0.3 nm). These two modes of operation provide two broad areas of capability for plasma spectroscopy. The first major application is measurement of emission line broadening; the second is emission line surveys from the ultraviolet to the near infrared. Measurements have been made on a low-temperature plasma produced by a miniature electrostatic plasma source and the high-temperature plasma in the MST Reversed-Field Pinch. The spectrometer is a modified Jarrell-Ash 0.5 m Ebert-Fastie monochromator. Light ismore » coupled into the entrance slit with a fused silica fiber optic bundle. The exposure time (2 ms minimum) is controlled by a fast electromechanical shutter. The exit plane detector is a compact and robust CCD detector developed for amateur astronomy by Santa Barbara Instrument Group. The CCD detector is controlled and read out by a Macintosh{reg_sign} computer. This spectrometer is sophisticated enough to serve well in a research laboratory, yet is simple and inexpensive enough to be affordable for instructional use.« less

Quadrilinear CCD sensors for the multispectral channel of spaceborne imagers

NASA Astrophysics Data System (ADS)

Materne, Alex; Gili, Bruno; Laubier, David; Gimenez, Thierry

2001-12-01

The PLEIADES-HR Earth Observation satellites will combine a high resolution panchromatic channel -- 0.7 m at nadir -- and a multispectral channel allowing a 2.8 m resolution. This paper presents the main specifications, design and performances of a 52 microns pitch quadrilinear CCD sensor developed by ATMEL under CNES contract, for the multispectral channel of the PLEIADES-HR instrument. The monolithic CCD device includes four lines of 1500 pixels, each line dedicated to a narrow spectral band within blue to near infra red spectrum. The design of the photodiodes and CCD registers, with larger size than those developed up to now for CNES spaceborne imagers, needed some specific structures to break the large equipotential areas where charge do not flow properly. Results are presented on the options which were experimented to improve sensitivity, maintain transfer efficiency and reduce power dissipation. The four spectral bands are achieved by four stripe filters made by SAGEM-REOSC PRODUCTS on a glass substrate, to be assembled on the sensor window. Line to line spacing on the silicon die takes into account the results of straylight analysis. A mineral layer, with high optical absorption performances is deposited between photosensitive lines to further reduce straylight.

Three-dimensional shape measurement system applied to superficial inspection of non-metallic pipes for the hydrocarbons transport

NASA Astrophysics Data System (ADS)

Arciniegas, Javier R.; González, Andrés. L.; Quintero, L. A.; Contreras, Carlos R.; Meneses, Jaime E.

2014-05-01

Three-dimensional shape measurement is a subject that consistently produces high scientific interest and provides information for medical, industrial and investigative applications, among others. In this paper, it is proposed to implement a three-dimensional (3D) reconstruction system for applications in superficial inspection of non-metallic pipes for the hydrocarbons transport. The system is formed by a CCD camera, a video-projector and a laptop and it is based on fringe projection technique. System functionality is evidenced by evaluating the quality of three-dimensional reconstructions obtained, which allow observing the failures and defects on the study object surface.

Transit timing at Toruń Center for Astronomy

NASA Astrophysics Data System (ADS)

Bykowski, W.; Maciejewski, G.

2011-01-01

The transit monitoring is one of well-known methods for discovering and observing new extrasolar planets. Among various advantages, this way of searching other worlds does not require complex and expensive equipment -- it can be performed with a relatively small telescope and high-quality CCD camera. At the Center for Astronomy of Nicolaus Copernicus University in Toruń, Poland, we collect observational data using the 60-cm Cassegrain telescope hoping that it would be possible to discover new objects in already known planetary systems using the transit timing variation method. Our observations are a part of a bigger cooperation between observatories from many countries.

Design and laboratory calibration of the compact pushbroom hyperspectral imaging system

NASA Astrophysics Data System (ADS)

Zhou, Jiankang; Ji, Yiqun; Chen, Yuheng; Chen, Xinhua; Shen, Weimin

2009-11-01

The designed hyperspectral imaging system is composed of three main parts, that is, optical subsystem, electronic subsystem and capturing subsystem. And a three-dimensional "image cube" can be obtained through push-broom. The fore-optics is commercial-off-the-shelf with high speed and three continuous zoom ratios. Since the dispersive imaging part is based on Offner relay configuration with an aberration-corrected convex grating, high power of light collection and variable view field are obtained. The holographic recording parameters of the convex grating are optimized, and the aberration of the Offner configuration dispersive system is balanced. The electronic system adopts module design, which can minimize size, mass, and power consumption. Frame transfer area-array CCD is chosen as the image sensor and the spectral line can be binned to achieve better SNR and sensitivity without any deterioration in spatial resolution. The capturing system based on the computer can set the capturing parameters, calibrate the spectrometer, process and display spectral imaging data. Laboratory calibrations are prerequisite for using precise spectral data. The spatial and spectral calibration minimize smile and keystone distortion caused by optical system, assembly and so on and fix positions of spatial and spectral line on the frame area-array CCD. Gases excitation lamp is used in smile calibration and the keystone calculation is carried out by different viewing field point source created by a series of narrow slit. The laboratory and field imaging results show that this pushbroom hyperspectral imaging system can acquire high quality spectral images.

Electronic still camera

NASA Astrophysics Data System (ADS)

Holland, S. Douglas

1992-09-01

A handheld, programmable, digital camera is disclosed that supports a variety of sensors and has program control over the system components to provide versatility. The camera uses a high performance design which produces near film quality images from an electronic system. The optical system of the camera incorporates a conventional camera body that was slightly modified, thus permitting the use of conventional camera accessories, such as telephoto lenses, wide-angle lenses, auto-focusing circuitry, auto-exposure circuitry, flash units, and the like. An image sensor, such as a charge coupled device ('CCD') collects the photons that pass through the camera aperture when the shutter is opened, and produces an analog electrical signal indicative of the image. The analog image signal is read out of the CCD and is processed by preamplifier circuitry, a correlated double sampler, and a sample and hold circuit before it is converted to a digital signal. The analog-to-digital converter has an accuracy of eight bits to insure accuracy during the conversion. Two types of data ports are included for two different data transfer needs. One data port comprises a general purpose industrial standard port and the other a high speed/high performance application specific port. The system uses removable hard disks as its permanent storage media. The hard disk receives the digital image signal from the memory buffer and correlates the image signal with other sensed parameters, such as longitudinal or other information. When the storage capacity of the hard disk has been filled, the disk can be replaced with a new disk.

Electronic Still Camera

NASA Technical Reports Server (NTRS)

Holland, S. Douglas (Inventor)

1992-01-01

A handheld, programmable, digital camera is disclosed that supports a variety of sensors and has program control over the system components to provide versatility. The camera uses a high performance design which produces near film quality images from an electronic system. The optical system of the camera incorporates a conventional camera body that was slightly modified, thus permitting the use of conventional camera accessories, such as telephoto lenses, wide-angle lenses, auto-focusing circuitry, auto-exposure circuitry, flash units, and the like. An image sensor, such as a charge coupled device ('CCD') collects the photons that pass through the camera aperture when the shutter is opened, and produces an analog electrical signal indicative of the image. The analog image signal is read out of the CCD and is processed by preamplifier circuitry, a correlated double sampler, and a sample and hold circuit before it is converted to a digital signal. The analog-to-digital converter has an accuracy of eight bits to insure accuracy during the conversion. Two types of data ports are included for two different data transfer needs. One data port comprises a general purpose industrial standard port and the other a high speed/high performance application specific port. The system uses removable hard disks as its permanent storage media. The hard disk receives the digital image signal from the memory buffer and correlates the image signal with other sensed parameters, such as longitudinal or other information. When the storage capacity of the hard disk has been filled, the disk can be replaced with a new disk.

Experimental research on femto-second laser damaging array CCD cameras

NASA Astrophysics Data System (ADS)

Shao, Junfeng; Guo, Jin; Wang, Ting-feng; Wang, Ming

2013-05-01

Charged Coupled Devices (CCD) are widely used in military and security applications, such as airborne and ship based surveillance, satellite reconnaissance and so on. Homeland security requires effective means to negate these advanced overseeing systems. Researches show that CCD based EO systems can be significantly dazzled or even damaged by high-repetition rate pulsed lasers. Here, we report femto - second laser interaction with CCD camera, which is probable of great importance in future. Femto - second laser is quite fresh new lasers, which has unique characteristics, such as extremely short pulse width (1 fs = 10-15 s), extremely high peak power (1 TW = 1012W), and especially its unique features when interacting with matters. Researches in femto second laser interaction with materials (metals, dielectrics) clearly indicate non-thermal effect dominates the process, which is of vast difference from that of long pulses interaction with matters. Firstly, the damage threshold test are performed with femto second laser acting on the CCD camera. An 800nm, 500μJ, 100fs laser pulse is used to irradiate interline CCD solid-state image sensor in the experiment. In order to focus laser energy onto tiny CCD active cells, an optical system of F/5.6 is used. A Sony production CCDs are chose as typical targets. The damage threshold is evaluated with multiple test data. Point damage, line damage and full array damage were observed when the irradiated pulse energy continuously increase during the experiment. The point damage threshold is found 151.2 mJ/cm2.The line damage threshold is found 508.2 mJ/cm2.The full-array damage threshold is found to be 5.91 J/cm2. Although the phenomenon is almost the same as that of nano laser interaction with CCD, these damage thresholds are substantially lower than that of data obtained from nano second laser interaction with CCD. Then at the same time, the electric features after different degrees of damage are tested with electronic multi meter. The resistance values between clock signal lines are measured. Contrasting the resistance values of the CCD before and after damage, it is found that the resistances decrease significantly between the vertical transfer clock signal lines values. The same results are found between the vertical transfer clock signal line and the earth electrode (ground).At last, the damage position and the damage mechanism were analyzed with above results and SEM morphological experiments. The point damage results in the laser destroying material, which shows no macro electro influence. The line damage is quite different from that of point damage, which shows deeper material corroding effect. More importantly, short circuits are found between vertical clock lines. The full array damage is even more severe than that of line damage starring with SEM, while no obvious different electrical features than that of line damage are found. Further researches are anticipated in femto second laser caused CCD damage mechanism with more advanced tools. This research is valuable in EO countermeasure and/or laser shielding applications.

Development of low-noise CCD drive electronics for the world space observatory ultraviolet spectrograph subsystem

NASA Astrophysics Data System (ADS)

Salter, Mike; Clapp, Matthew; King, James; Morse, Tom; Mihalcea, Ionut; Waltham, Nick; Hayes-Thakore, Chris

2016-07-01

World Space Observatory Ultraviolet (WSO-UV) is a major Russian-led international collaboration to develop a large space-borne 1.7 m Ritchey-Chrétien telescope and instrumentation to study the universe at ultraviolet wavelengths between 115 nm and 320 nm, exceeding the current capabilities of ground-based instruments. The WSO Ultraviolet Spectrograph subsystem (WUVS) is led by the Institute of Astronomy of the Russian Academy of Sciences and consists of two high resolution spectrographs covering the Far-UV range of 115-176 nm and the Near-UV range of 174-310 nm, and a long-slit spectrograph covering the wavelength range of 115-305 nm. The custom-designed CCD sensors and cryostat assemblies are being provided by e2v technologies (UK). STFC RAL Space is providing the Camera Electronics Boxes (CEBs) which house the CCD drive electronics for each of the three WUVS channels. This paper presents the results of the detailed characterisation of the WUVS CCD drive electronics. The electronics include a novel high-performance video channel design that utilises Digital Correlated Double Sampling (DCDS) to enable low-noise readout of the CCD at a range of pixel frequencies, including a baseline requirement of less than 3 electrons rms readout noise for the combined CCD and electronics system at a readout rate of 50 kpixels/s. These results illustrate the performance of this new video architecture as part of a wider electronics sub-system that is designed for use in the space environment. In addition to the DCDS video channels, the CEB provides all the bias voltages and clocking waveforms required to operate the CCD and the system is fully programmable via a primary and redundant SpaceWire interface. The development of the CEB electronics design has undergone critical design review and the results presented were obtained using the engineering-grade electronics box. A variety of parameters and tests are included ranging from general system metrics, such as the power and mass, to more detailed analysis of the video performance including noise, linearity, crosstalk, gain stability and transient response.

Performance of 4x5120 Element Visible and 2x2560 Element Shortwave Infrared Multispectral Focal Planes

NASA Astrophysics Data System (ADS)

Tower, J. R.; Cope, A. D.; Pellion, L. E.; McCarthy, B. M.; Strong, R. T.; Kinnard, K. F.; Moldovan, A. G.; Levine, P. A.; Elabd, H.; Hoffman, D. M.

1985-12-01

Performance measurements of two Multispectral Linear Array focal planes are presented. Both pushbroom sensors have been developed for application in remote sensing instruments. A buttable, four-spectral-band, linear-format charge coupled device (CCD) and a but-table, two-spectral-band, linear-format, shortwave infrared charge coupled device (IRCCD) have been developed under NASA funding. These silicon integrated circuits may be butted end to end to provide very-high-resolution multispectral focal planes. The visible CCD is organized as four sensor lines of 1024 pixels each. Each line views the scene in a different spectral window defined by integral optical bandpass filters. A prototype focal plane with five devices, providing 4x5120-pixel resolution has been demonstrated. The high quantum efficiency of the backside-illuminated CCD technology provides excellent signal-to-noise performance and unusually high MTF across the entire visible and near-IR spectrum. The shortwave infrared (SWIR) sensor is organized as two line sensors of 512 detectors each. The SWIR (1-2.5 μm) spectral windows may be defined by bandpass filters placed in close proximity to the devices. The dual-band sensor consists of Schottky barrier detectors read out by CCD multiplexers. This monolithic sensor operates at 125°K with radiometric performance. A prototype five-device focal plane providing 2x2560 detectors has been demonstrated. The devices provide very high uniformity, and excellent MTF across the SWIR band.

Use and validation of mirrorless digital single light reflex camera for recording of vitreoretinal surgeries in high definition

PubMed Central

Khanduja, Sumeet; Sampangi, Raju; Hemlatha, B C; Singh, Satvir; Lall, Ashish

2018-01-01

Purpose: The purpose of this study is to describe the use of commercial digital single light reflex (DSLR) for vitreoretinal surgery recording and compare it to standard 3-chip charged coupling device (CCD) camera. Methods: Simultaneous recording was done using Sony A7s2 camera and Sony high-definition 3-chip camera attached to each side of the microscope. The videos recorded from both the camera systems were edited and sequences of similar time frames were selected. Three sequences that selected for evaluation were (a) anterior segment surgery, (b) surgery under direct viewing system, and (c) surgery under indirect wide-angle viewing system. The videos of each sequence were evaluated and rated on a scale of 0-10 for color, contrast, and overall quality Results: Most results were rated either 8/10 or 9/10 for both the cameras. A noninferiority analysis by comparing mean scores of DSLR camera versus CCD camera was performed and P values were obtained. The mean scores of the two cameras were comparable for each other on all parameters assessed in the different videos except of color and contrast in posterior pole view and color on wide-angle view, which were rated significantly higher (better) in DSLR camera. Conclusion: Commercial DSLRs are an affordable low-cost alternative for vitreoretinal surgery recording and may be used for documentation and teaching. PMID:29283133

Use and validation of mirrorless digital single light reflex camera for recording of vitreoretinal surgeries in high definition.

PubMed

Khanduja, Sumeet; Sampangi, Raju; Hemlatha, B C; Singh, Satvir; Lall, Ashish

2018-01-01

The purpose of this study is to describe the use of commercial digital single light reflex (DSLR) for vitreoretinal surgery recording and compare it to standard 3-chip charged coupling device (CCD) camera. Simultaneous recording was done using Sony A7s2 camera and Sony high-definition 3-chip camera attached to each side of the microscope. The videos recorded from both the camera systems were edited and sequences of similar time frames were selected. Three sequences that selected for evaluation were (a) anterior segment surgery, (b) surgery under direct viewing system, and (c) surgery under indirect wide-angle viewing system. The videos of each sequence were evaluated and rated on a scale of 0-10 for color, contrast, and overall quality Results: Most results were rated either 8/10 or 9/10 for both the cameras. A noninferiority analysis by comparing mean scores of DSLR camera versus CCD camera was performed and P values were obtained. The mean scores of the two cameras were comparable for each other on all parameters assessed in the different videos except of color and contrast in posterior pole view and color on wide-angle view, which were rated significantly higher (better) in DSLR camera. Commercial DSLRs are an affordable low-cost alternative for vitreoretinal surgery recording and may be used for documentation and teaching.

Deflection Measurements of a Thermally Simulated Nuclear Core Using a High-Resolution CCD-Camera

NASA Technical Reports Server (NTRS)

Stanojev, B. J.; Houts, M.

2004-01-01

Space fission systems under consideration for near-term missions all use compact. fast-spectrum reactor cores. Reactor dimensional change with increasing temperature, which affects neutron leakage. is the dominant source of reactivity feedback in these systems. Accurately measuring core dimensional changes during realistic non-nuclear testing is therefore necessary in predicting the system nuclear equivalent behavior. This paper discusses one key technique being evaluated for measuring such changes. The proposed technique is to use a Charged Couple Device (CCD) sensor to obtain deformation readings of electrically heated prototypic reactor core geometry. This paper introduces a technique by which a single high spatial resolution CCD camera is used to measure core deformation in Real-Time (RT). Initial system checkout results are presented along with a discussion on how additional cameras could be used to achieve a three- dimensional deformation profile of the core during test.

Solar x ray astronomy rocket program

NASA Technical Reports Server (NTRS)

1990-01-01

The dynamics were studied of the solar corona through the imaging of large scale coronal structures with AS&E High Resolution Soft X ray Imaging Solar Sounding Rocket Payload. The proposal for this program outlined a plan of research based on the construction of a high sensitivity X ray telescope from the optical and electronic components of the previous flight of this payload (36.038CS). Specifically, the X ray sensitive CCD camera was to be placed in the prime focus of the grazing incidence X ray mirror. The improved quantum efficiency of the CCD detector (over the film which had previously been used) allows quantitative measurements of temperature and emission measure in regions of low x ray emission such as helmet streamers beyond 1.2 solar radii or coronal holes. Furthermore, the improved sensitivity of the CCD allows short exposures of bright objects to study unexplored temporal regimes of active region loop evolution.

Inexpensive Neutron Imaging Cameras Using CCDs for Astronomy

NASA Astrophysics Data System (ADS)

Hewat, A. W.

We have developed inexpensive neutron imaging cameras using CCDs originally designed for amateur astronomical observation. The low-light, high resolution requirements of such CCDs are similar to those for neutron imaging, except that noise as well as cost is reduced by using slower read-out electronics. For example, we use the same 2048x2048 pixel ;Kodak; KAI-4022 CCD as used in the high performance PCO-2000 CCD camera, but our electronics requires ∼5 sec for full-frame read-out, ten times slower than the PCO-2000. Since neutron exposures also require several seconds, this is not seen as a serious disadvantage for many applications. If higher frame rates are needed, the CCD unit on our camera can be easily swapped for a faster readout detector with similar chip size and resolution, such as the PCO-2000 or the sCMOS PCO.edge 4.2.

[Orthodontic and oral surgery therapy in cleidocranial dysplasia].

PubMed

Balaton, Gergely; Tarján, Ildikó; Balaton, Péter; Barabási, Zoltán; Gyulai Gál, Szabolcs; Nagy, Katalin; Vajó, Zoltán

2007-02-01

A cleidocranial dysplasia is an autosomal dominant inherited condition consisting of generalized skeletal disorder. Associated dental signs are present in 93,5%; failure of tooth eruption with multiple supernumerary teeth, dilaceration of roots, crown germination, microdontia, high arched palate, midface hypoplasia, high gonion angle. The molecular- genetic analysis revealed a missense mutation in the CBFA1 gene located on chromosome 6p21, which is considered to be etiological factor for CCD. Orthodontic and oral surgery therapy of a 13 year-old child with CCD was performed due to aesthetic and functional problems. The supernumerary germs were removed and the teeth were aligned with orthodontic appliances. Temporary functional rehabilitation was solved with partial denture. The presented case and the literature data support the importance of early diagnosis of CCD. The good collaboration of the orthodontic and maxillo-facial surgery specialists help achieve the correct rehabilitation of the patient.

Ultrasound-modulated optical tomography with intense acoustic bursts.

PubMed

Zemp, Roger J; Kim, Chulhong; Wang, Lihong V

2007-04-01

Ultrasound-modulated optical tomography (UOT) detects ultrasonically modulated light to spatially localize multiply scattered photons in turbid media with the ultimate goal of imaging the optical properties in living subjects. A principal challenge of the technique is weak modulated signal strength. We discuss ways to push the limits of signal enhancement with intense acoustic bursts while conforming to optical and ultrasonic safety standards. A CCD-based speckle-contrast detection scheme is used to detect acoustically modulated light by measuring changes in speckle statistics between ultrasound-on and ultrasound-off states. The CCD image capture is synchronized with the ultrasound burst pulse sequence. Transient acoustic radiation force, a consequence of bursts, is seen to produce slight signal enhancement over pure ultrasonic-modulation mechanisms for bursts and CCD exposure times of the order of milliseconds. However, acoustic radiation-force-induced shear waves are launched away from the acoustic sample volume, which degrade UOT spatial resolution. By time gating the CCD camera to capture modulated light before radiation force has an opportunity to accumulate significant tissue displacement, we reduce the effects of shear-wave image degradation, while enabling very high signal-to-noise ratios. Additionally, we maintain high-resolution images representative of optical and not mechanical contrast. Signal-to-noise levels are sufficiently high so as to enable acquisition of 2D images of phantoms with one acoustic burst per pixel.

Composite x-ray image assembly for large-field digital mammography with one- and two-dimensional positioning of a focal plane array

NASA Technical Reports Server (NTRS)

Halama, G.; McAdoo, J.; Liu, H.

1998-01-01

To demonstrate the feasibility of a novel large-field digital mammography technique, a 1024 x 1024 pixel Loral charge-coupled device (CCD) focal plane array (FPA) was positioned in a mammographic field with one- and two-dimensional scan sequences to obtain 950 x 1800 pixel and 3600 x 3600 pixel composite images, respectively. These experiments verify that precise positioning of FPAs produced seamless composites and that the CCD mosaic concept has potential for high-resolution, large-field imaging. The proposed CCD mosaic concept resembles a checkerboard pattern with spacing left between the CCDs for the driver and readout electronics. To obtain a complete x-ray image, the mosaic must be repositioned four times, with an x-ray exposure at each position. To reduce the patient dose, a lead shield with appropriately patterned holes is placed between the x-ray source and the patient. The high-precision motorized translation stages and the fiber-coupled-scintillating-screen-CCD sensor assembly were placed in the position usually occupied by the film cassette. Because of the high mechanical precision, seamless composites were constructed from the subimages. This paper discusses the positioning, image alignment procedure, and composite image results. The paper only addresses the formation of a seamless composite image from subimages and will not consider the effects of the lead shield, multiple CCDs, or the speed of motion.

Double and Multiple Star Measurements in the Northern Sky with a 10" Newtonian and a Fast CCD Camera in 2006 through 2009

NASA Astrophysics Data System (ADS)

Anton, Rainer

2010-07-01

Using a 10" Newtonian and a fast CCD camera, recordings of double and multiple stars were made at high frame rates with a notebook computer. From superpositions of "lucky images", measurements of 139 systems were obtained and compared with literature data. B/w and color images of some noteworthy systems are also presented.

Bioinformatic and expression analyses on carotenoid dioxygenase genes in fruit development and abiotic stress responses in Fragaria vesca.

PubMed

Wang, Yong; Ding, Guanqun; Gu, Tingting; Ding, Jing; Li, Yi

2017-08-01

Carotenoid dioxygenases, including 9-cis-epoxycarotenoid dioxygenases (NCEDs) and carotenoid cleavage dioxygenases (CCDs), can selectively cleave carotenoids into various apocarotenoid products that play important roles in fleshy fruit development and abiotic stress response. In this study, we identified 12 carotenoid dioxygenase genes in diploid strawberry Fragaria vesca, and explored their evolution with orthologous genes from nine other species. Phylogenetic analyses suggested that the NCED and CCDL groups moderately expanded during their evolution, whereas gene numbers of the CCD1, CCD4, CCD7, and CCD8 groups maintained conserved. We characterized the expression profiles of FveNCED and FveCCD genes during flower and fruit development, and in response to several abiotic stresses. FveNCED1 expression positively responded to osmotic, cold, and heat stresses, whereas FveNCED2 was only induced under cold stress. In contrast, FveNCED2 was the unique gene highly and continuously increasing in receptacle during fruit ripening, which co-occurred with the increase in endogenous abscisic acid (ABA) content previously reported in octoploid strawberry. The differential expression patterns suggested that FveNCED1 and FveNCED2 were key genes for ABA biosynthesis in abiotic stress responses and fruit ripening, respectively. FveCCD1 exhibited the highest expression in most stages of flower and fruit development, while the other FveCCDs were expressed in a subset of stages and tissues. Our study suggests distinct functions of FveNCED and FveCCD genes in fruit development and stress responses and lays a foundation for future study to understand the roles of these genes and their metabolites, including ABA and other apocarotenoid products, in the growth and development of strawberry.

Transgenic mice expressing mutant Pinin exhibit muscular dystrophy, nebulin deficiency and elevated expression of slow-type muscle fiber genes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, Hsu-Pin; Hsu, Shu-Yuan; Wu, Wen-Ai

Highlights: •Pnn CCD domain functions as a dominant negative mutant regulating Pnn expression and function. •Pnn CCD mutant Tg mice have a muscle wasting phenotype during development and show dystrophic histological features. •Pnn mutant muscles are susceptible to slow fiber type gene transition and NEB reduction. •The Tg mouse generated by overexpression of the Pnn CCD domain displays many characteristics resembling NEB{sup +/−} mice. -- Abstract: Pinin (Pnn) is a nuclear speckle-associated SR-like protein. The N-terminal region of the Pnn protein sequence is highly conserved from mammals to insects, but the C-terminal RS domain-containing region is absent in lower species.more » The N-terminal coiled-coil domain (CCD) is, therefore, of interest not only from a functional point of view, but also from an evolutionarily standpoint. To explore the biological role of the Pnn CCD in a physiological context, we generated transgenic mice overexpressing Pnn mutant in skeletal muscle. We found that overexpression of the CCD reduces endogenous Pnn expression in cultured cell lines as well as in transgenic skeletal muscle fibers. Pnn mutant mice exhibited reduced body mass and impaired muscle function during development. Mutant skeletal muscles show dystrophic histological features with muscle fibers heavily loaded with centrally located myonuclei. Expression profiling and pathway analysis identified over-representation of genes in gene categories associated with muscle contraction, specifically those related to slow type fiber. In addition nebulin (NEB) expression level is repressed in Pnn mutant skeletal muscle. We conclude that Pnn downregulation in skeletal muscle causes a muscular dystrophic phenotype associated with NEB deficiency and the CCD domain is incapable of replacing full length Pnn in terms of functional capacity.« less

Smartwatch feedback device for high-quality chest compressions by a single rescuer during infant cardiac arrest: a randomized, controlled simulation study.

PubMed

Lee, Juncheol; Song, Yeongtak; Oh, Jaehoon; Chee, Youngjoon; Ahn, Chiwon; Shin, Hyungoo; Kang, Hyunggoo; Lim, Tae Ho

2018-02-12

According to the guidelines, rescuers should provide chest compressions (CC) ∼1.5 inches (40 mm) for infants. Feedback devices could help rescuers perform CC with adequate rates (CCR) and depths (CCD). However, there is no CC feedback device for infant cardiopulmonary resuscitation (CPR). We suggest a smartwatch-based CC feedback application for infant CPR. We created a smartwatch-based CC feedback application. This application provides feedback on CCD and CCR by colour and text for infant CPR. To evaluate the application, 30 participants were divided randomly into two groups on the basis of whether CC was performed with or without the assistance of the smartwatch application. Both groups performed continuous CC-only CPR for 2 min on an infant mannequin placed on a firm table. We collected CC parameters from the mannequin, including the proportion of correct depth, CCR, CCD and the proportion of correct decompression depth. Demographics between the two groups were not significantly different. The median (interquartile range) proportion of correct depth was 99 (97-100) with feedback compared with 83 (58-97) without feedback (P=0.002). The CCR and proportion of correct decompression depth were not significantly different between the two groups (P=0.482 and 0.089). The CCD of the feedback group was significantly deeper than that of the control group [feedback vs. 41.2 (39.8-41.7) mm vs. 38.6 (36.1-39.6) mm; P=0.004]. Rescuers who receive feedback of CC parameters from a smartwatch could perform adequate CC during infant CPR.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/.

The impact of radiation damage on photon counting with an EMCCD for the WFIRST-AFTA coronagraph

NASA Astrophysics Data System (ADS)

Bush, Nathan; Hall, David; Holland, Andrew; Burgon, Ross; Murray, Neil; Gow, Jason; Soman, Matthew; Jordan, Douglas; Demers, Richard; Harding, Leon; Hoenk, Michael; Michaels, Darren; Nemati, Bijan; Peddada, Pavani

2015-09-01

WFIRST-AFTA is a 2.4m class NASA observatory designed to address a wide range of science objectives using two complementary scientific payloads. The Wide Field Instrument (WFI) offers Hubble quality imaging over a 0.28 square degree field of view, and will gather NIR statistical data on exoplanets through gravitational microlensing. The second instrument is a high contrast coronagraph that will carry out the direct imaging and spectroscopic analysis of exoplanets, providing a means to probe the structure and composition of planetary systems. The coronagraph instrument is expected to operate in low photon flux for long integration times, meaning all noise sources must be kept to a minimum. In order to satisfy the low noise requirements, the Electron Multiplication (EM)-CCD has been baselined for both the imaging and spectrograph cameras. The EMCCD was selected in comparison with other candidates because of its low effective electronic read noise at sub-electron values with appropriate multiplication gain setting. The presence of other noise sources, however, such as thermal dark signal and Clock Induced Charge (CIC), need to be characterised and mitigated. In addition, operation within a space environment will subject the device to radiation damage that will degrade the Charge Transfer Efficiency (CTE) of the device throughout the mission lifetime. Here we present our latest results from pre- and post-irradiation testing of the e2v CCD201-20 BI EMCCD sensor, baselined for the WFIRST-AFTA coronagraph instrument. A description of the detector technology is presented, alongside considerations for operation within a space environment. The results from a room temperature irradiation are discussed in context with the nominal operating requirements of AFTA-C and future work which entails a cryogenic irradiation of the CCD201-20 is presented.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aoyagi, Yasuyuki; Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, Chiba; Kuroda, Masayuki, E-mail: kurodam@faculty.chiba-u.jp

Adipose tissue is expected to provide a source of cells for protein replacement therapies via auto-transplantation. However, the conditioning of the environment surrounding the transplanted adipocytes for their long-term survival and protein secretion properties has not been established. We have recently developed a preparation procedure for preadipocytes, ceiling culture-derived proliferative adipocytes (ccdPAs), as a therapeutic gene vehicle suitable for stable gene product secretion. We herein report the results of our evaluation of using fibrin glue as a scaffold for the transplanted ccdPAs for the expression of a transduced gene in a three-dimensional culture system. The ccdPAs secreted the functional proteinmore » translated from an exogenously transduced gene, as well as physiological adipocyte proteins, and the long viability of ccdPAs (up to 84 days) was dependent on the fibrinogen concentrations. The ccdPAs spontaneously accumulated lipid droplets, and their expression levels of the transduced exogenous gene with its product were maintained for at least 56 days. The fibrinogen concentration modified the adipogenic differentiation of ccdPAs and their exogenous gene expression levels, and the levels of exogenously transduced gene expression at the different fibrinogen concentrations were dependent on the extent of adipogenic differentiation in the gel. These results indicate that fibrin glue helps to maintain the high adipogenic potential of cultured adipocytes after passaging in a 3D culture system, and suggests that once they are successfully implanted at the transplantation site, the cells exhibit increased expression of the transduced gene with adipogenic differentiation.« less

The Cross-Cultural Dementia Screening (CCD): A new neuropsychological screening instrument for dementia in elderly immigrants.

PubMed

Goudsmit, Miriam; Uysal-Bozkir, Özgül; Parlevliet, Juliette L; van Campen, Jos P C M; de Rooij, Sophia E; Schmand, Ben

2017-03-01

Currently, approximately 3.9% of the European population are non-EU citizens, and a large part of these people are from "non-Western" societies, such as Turkey and Morocco. For various reasons, the incidence of dementia in this group is expected to increase. However, cognitive testing is challenging due to language barriers and low education and/or illiteracy. The newly developed Cross-Cultural Dementia Screening (CCD) can be administered without an interpreter. It contains three subtests that assess memory, mental speed, and executive function. We hypothesized the CCD to be a culture-fair test that could discriminate between demented patients and cognitively healthy controls. To test this hypothesis, 54 patients who had probable dementia were recruited via memory clinics. Controls (N = 1625) were recruited via their general practitioners. All patients and controls were aged 55 years and older and of six different self-defined ethnicities (Dutch, Turkish, Moroccan-Arabic, Moroccan-Berber, Surinamese-Creole, and Surinamese-Hindustani). Exclusion criteria included current or previous conditions that affect cognitive functioning. There were performance differences between the ethnic groups, but these disappeared after correcting for age and education differences between the groups, which supports our central hypothesis that the CCD is a culture-fair test. Receiver-operating characteristic (ROC) and logistic regression analyses showed that the CCD has high predictive validity for dementia (sensitivity: 85%; specificity: 89%). The CCD is a sensitive and culture-fair neuropsychological instrument for dementia screening in low-educated immigrant populations.

Event-Driven Random-Access-Windowing CCD Imaging System

NASA Technical Reports Server (NTRS)

Monacos, Steve; Portillo, Angel; Ortiz, Gerardo; Alexander, James; Lam, Raymond; Liu, William

2004-01-01

A charge-coupled-device (CCD) based high-speed imaging system, called a realtime, event-driven (RARE) camera, is undergoing development. This camera is capable of readout from multiple subwindows [also known as regions of interest (ROIs)] within the CCD field of view. Both the sizes and the locations of the ROIs can be controlled in real time and can be changed at the camera frame rate. The predecessor of this camera was described in High-Frame-Rate CCD Camera Having Subwindow Capability (NPO- 30564) NASA Tech Briefs, Vol. 26, No. 12 (December 2002), page 26. The architecture of the prior camera requires tight coupling between camera control logic and an external host computer that provides commands for camera operation and processes pixels from the camera. This tight coupling limits the attainable frame rate and functionality of the camera. The design of the present camera loosens this coupling to increase the achievable frame rate and functionality. From a host computer perspective, the readout operation in the prior camera was defined on a per-line basis; in this camera, it is defined on a per-ROI basis. In addition, the camera includes internal timing circuitry. This combination of features enables real-time, event-driven operation for adaptive control of the camera. Hence, this camera is well suited for applications requiring autonomous control of multiple ROIs to track multiple targets moving throughout the CCD field of view. Additionally, by eliminating the need for control intervention by the host computer during the pixel readout, the present design reduces ROI-readout times to attain higher frame rates. This camera (see figure) includes an imager card consisting of a commercial CCD imager and two signal-processor chips. The imager card converts transistor/ transistor-logic (TTL)-level signals from a field programmable gate array (FPGA) controller card. These signals are transmitted to the imager card via a low-voltage differential signaling (LVDS) cable assembly. The FPGA controller card is connected to the host computer via a standard peripheral component interface (PCI).

Design of multi-mode compatible image acquisition system for HD area array CCD

NASA Astrophysics Data System (ADS)

Wang, Chen; Sui, Xiubao

2014-11-01

Combining with the current development trend in video surveillance-digitization and high-definition, a multimode-compatible image acquisition system for HD area array CCD is designed. The hardware and software designs of the color video capture system of HD area array CCD KAI-02150 presented by Truesense Imaging company are analyzed, and the structure parameters of the HD area array CCD and the color video gathering principle of the acquisition system are introduced. Then, the CCD control sequence and the timing logic of the whole capture system are realized. The noises of the video signal (KTC noise and 1/f noise) are filtered by using the Correlated Double Sampling (CDS) technique to enhance the signal-to-noise ratio of the system. The compatible designs in both software and hardware for the two other image sensors of the same series: KAI-04050 and KAI-08050 are put forward; the effective pixels of these two HD image sensors are respectively as many as four million and eight million. A Field Programmable Gate Array (FPGA) is adopted as the key controller of the system to perform the modularization design from top to bottom, which realizes the hardware design by software and improves development efficiency. At last, the required time sequence driving is simulated accurately by the use of development platform of Quartus II 12.1 combining with VHDL. The result of the simulation indicates that the driving circuit is characterized by simple framework, low power consumption, and strong anti-interference ability, which meet the demand of miniaturization and high-definition for the current tendency.

Lethality Prediction for Escherichia Coli O157:H7 and Uropathogenic E. coli in Ground Chicken Treated with High Pressure Processing and Trans-Cinnamaldehyde.

PubMed

Sheen, Shiowshuh; Huang, Chi-Yun; Ramos, Rommel; Chien, Shih-Yung; Scullen, O Joseph; Sommers, Christopher

2018-03-01

Pathogenic Escherichia coli, intestinal (O157:H7) as well as extraintestinal types (for example, Uropathogenic E. coli [UPEC]) are commonly found in many foods including raw chicken meat. The resistance of E. coli O157:H7 to UPEC in chicken meat under the stresses of high hydrostatic Pressure (HHP, also known as HPP-high pressure processing) and trans-cinnamaldehyde (an essential oil) was investigated and compared. UPEC was found slightly less resistant than O157:H7 in our test parameter ranges. With the addition of trans-cinnamaldehyde as an antimicrobial to meat, HPP lethality enhanced both O157:H7 and UPEC inactivation. To facilitate the predictive model development, a central composite design (CCD) was used to assess the 3-parameter effects, that is, pressure (300 to 400 MPa), trans-cinnamaldehyde dose (0.2 to 0.5%, w/w), and pressure-holding time (15 to 25 min), on the inactivation of E. coli O157:H7 and UPEC in ground chicken. Linear models were developed to estimate the lethality of E. coli O157:H7 (R 2 = 0.86) and UPEC (R 2 = 0.85), as well as dimensionless nonlinear models. All models were validated with data obtained from separated CCD combinations. Because linear models of O157:H7 and UPEC had similar R 2 and the significant lethality difference of CCD points was only 9 in 20; all data were combined to generate models to include both O157:H7 and UPEC. The results provide useful information/tool to predict how pathogenic E. coli may survive HPP in the presence of trans-cinnamaldehyde and to achieve a great than 5 log CFU/g reduction in chicken meat. The models may be used for process optimization, product development and to assist the microbial risk assessment. The study provided an effective means to reduce the high hydrostatic pressure level with incorporation of antimicrobial compound to achieve a 5-log reduction of pathogenic E. coli without damaging the raw meat quality. The developed models may be used to predict the high pressure processing lethality (and process optimization), product development (ingredient selection), and to assist the microbial risk assessment. © 2018 Institute of Food Technologists®.

Photosensor with enhanced quantum efficiency

NASA Technical Reports Server (NTRS)

Janesick, James R. (Inventor); Elliott, Stythe T. (Inventor)

1989-01-01

A method to significantly increase the quantum efficiency (QE) of a CCD (or similar photosensor) applied in the UV, far UV and low energy x-ray regions of the spectrum. The increase in QE is accomplished by overthinning the backside of a CCD substrate beyond the epitaxial interface and UV flooding the sensor prior to use. The UV light photoemits electrons to the thinned surface and charges the backside negatively. This in turn forms an accumulation layer of holes near the Si-SiO.sub.2 interface creating an electric field gradient in the silicon which directs the photogenerated signal to the frontside where they are collected in pixel locations and later transferred. An oxide film, in which the backside charge resides, must have quality equivalent to a well aged native oxide which typically takes several years to form under ambient conditions. To reduce the amount of time in growing an oxide of sufficient quality, a process has been developed to grow an oxide by using deionized steam at 95.degree. C. which takes less than one hour to grow.

Developing a CCD camera with high spatial resolution for RIXS in the soft X-ray range

NASA Astrophysics Data System (ADS)

Soman, M. R.; Hall, D. J.; Tutt, J. H.; Murray, N. J.; Holland, A. D.; Schmitt, T.; Raabe, J.; Schmitt, B.

2013-12-01

The Super Advanced X-ray Emission Spectrometer (SAXES) at the Swiss Light Source contains a high resolution Charge-Coupled Device (CCD) camera used for Resonant Inelastic X-ray Scattering (RIXS). Using the current CCD-based camera system, the energy-dispersive spectrometer has an energy resolution (E/ΔE) of approximately 12,000 at 930 eV. A recent study predicted that through an upgrade to the grating and camera system, the energy resolution could be improved by a factor of 2. In order to achieve this goal in the spectral domain, the spatial resolution of the CCD must be improved to better than 5 μm from the current 24 μm spatial resolution (FWHM). The 400 eV-1600 eV energy X-rays detected by this spectrometer primarily interact within the field free region of the CCD, producing electron clouds which will diffuse isotropically until they reach the depleted region and buried channel. This diffusion of the charge leads to events which are split across several pixels. Through the analysis of the charge distribution across the pixels, various centroiding techniques can be used to pinpoint the spatial location of the X-ray interaction to the sub-pixel level, greatly improving the spatial resolution achieved. Using the PolLux soft X-ray microspectroscopy endstation at the Swiss Light Source, a beam of X-rays of energies from 200 eV to 1400 eV can be focused down to a spot size of approximately 20 nm. Scanning this spot across the 16 μm square pixels allows the sub-pixel response to be investigated. Previous work has demonstrated the potential improvement in spatial resolution achievable by centroiding events in a standard CCD. An Electron-Multiplying CCD (EM-CCD) has been used to improve the signal to effective readout noise ratio achieved resulting in a worst-case spatial resolution measurement of 4.5±0.2 μm and 3.9±0.1 μm at 530 eV and 680 eV respectively. A method is described that allows the contribution of the X-ray spot size to be deconvolved from these worst-case resolution measurements, estimating the spatial resolution to be approximately 3.5 μm and 3.0 μm at 530 eV and 680 eV, well below the resolution limit of 5 μm required to improve the spectral resolution by a factor of 2.

Functional characterisation of three members of the Vitis vinifera L. carotenoid cleavage dioxygenase gene family

PubMed Central

2013-01-01

Background In plants, carotenoids serve as the precursors to C13-norisoprenoids, a group of apocarotenoid compounds with diverse biological functions. Enzymatic cleavage of carotenoids catalysed by members of the carotenoid cleavage dioxygenase (CCD) family has been shown to produce a number of industrially important volatile flavour and aroma apocarotenoids including β-ionone, geranylacetone, pseudoionone, α-ionone and 3-hydroxy-β-ionone in a range of plant species. Apocarotenoids contribute to the floral and fruity attributes of many wine cultivars and are thereby, at least partly, responsible for the “varietal character”. Despite their importance in grapes and wine; carotenoid cleavage activity has only been described for VvCCD1 and the mechanism(s) and regulation of carotenoid catabolism remains largely unknown. Results Three grapevine-derived CCD-encoding genes have been isolated and shown to be functional with unique substrate cleavage capacities. Our results demonstrate that the VvCCD4a and VvCCD4b catalyse the cleavage of both linear and cyclic carotenoid substrates. The expression of VvCCD1, VvCCD4a and VvCCD4b was detected in leaf, flower and throughout berry development. VvCCD1 expression was constitutive, whereas VvCCD4a expression was predominant in leaves and VvCCD4b in berries. A transgenic population with a 12-fold range of VvCCD1 expression exhibited a lack of correlation between VvCCD1 expression and carotenoid substrates and/or apocarotenoid products in leaves, providing proof that the in planta function(s) of VvCCD1 in photosynthetically active tissue is distinct from the in vitro activities demonstrated. The isolation and functional characterisation of VvCCD4a and VvCCD4b identify two additional CCDs that are functional in grapevine. Conclusions Taken together, our results indicate that the three CCDs are under various levels of control that include gene expression (spatial and temporal), substrate specificity and compartmentalisation that act individually and/or co-ordinately to maintain carotenoid and volatile apocarotenoid levels in plants. Altering the expression of VvCCD1 in a transgenic grapevine population illustrated the divergence between the in vitro enzyme activity and the in planta activity of this enzyme, thereby contributing to the efforts to understand how enzymatic degradation of carotenoids involved in photosynthesis occurs. The identification and functional characterisation of VvCCD4a and VvCCD4b suggest that these enzymes are primarily responsible for catalysing the cleavage of plastidial carotenoids. PMID:24106789

A compact high-speed pnCCD camera for optical and x-ray applications

NASA Astrophysics Data System (ADS)

Ihle, Sebastian; Ordavo, Ivan; Bechteler, Alois; Hartmann, Robert; Holl, Peter; Liebel, Andreas; Meidinger, Norbert; Soltau, Heike; Strüder, Lothar; Weber, Udo

2012-07-01

We developed a camera with a 264 × 264 pixel pnCCD of 48 μm size (thickness 450 μm) for X-ray and optical applications. It has a high quantum efficiency and can be operated up to 400 / 1000 Hz (noise≍ 2:5 ° ENC / ≍4:0 ° ENC). High-speed astronomical observations can be performed with low light levels. Results of test measurements will be presented. The camera is well suitable for ground based preparation measurements for future X-ray missions. For X-ray single photons, the spatial position can be determined with significant sub-pixel resolution.

Line scanning system for direct digital chemiluminescence imaging of DNA sequencing blots

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karger, A.E.; Weiss, R.; Gesteland, R.F.

A cryogenically cooled charge-coupled device (CCD) camera equipped with an area CCD array is used in a line scanning system for low-light-level imaging of chemiluminescent DNA sequencing blots. Operating the CCD camera in time-delayed integration (TDI) mode results in continuous data acquisition independent of the length of the CCD array. Scanning is possible with a resolution of 1.4 line pairs/mm at the 50% level of the modulation transfer function. High-sensitivity, low-light-level scanning of chemiluminescent direct-transfer electrophoresis (DTE) DNA sequencing blots is shown. The detection of DNA fragments on the blot involves DNA-DNA hybridization with oligonucleotide-alkaline phosphatase conjugate and 1,2-dioxetane-based chemiluminescence.more » The width of the scan allows the recording of up to four sequencing reactions (16 lanes) on one scan. The scan speed of 52 cm/h used for the sequencing blots corresponds to a data acquisition rate of 384 pixels/s. The chemiluminescence detection limit on the scanned images is 3.9 [times] 10[sup [minus]18] mol of plasmid DNA. A conditional median filter is described to remove spikes caused by cosmic ray events from the CCD images. 39 refs., 9 refs.« less

Tomato carotenoid cleavage dioxygenases 1A and 1B: Relaxed double bond specificity leads to a plenitude of dialdehydes, mono-apocarotenoids and isoprenoid volatiles

PubMed Central

Ilg, Andrea; Bruno, Mark; Beyer, Peter; Al-Babili, Salim

2014-01-01

The biosynthetic processes leading to many of the isoprenoid volatiles released by tomato fruits are still unknown, though previous reports suggested a clear correlation with the carotenoids contained within the fruit. In this study, we investigated the activity of the tomato (Solanum lycopersicum) carotenoid cleavage dioxygenase (SlCCD1B), which is highly expressed in fruits, and of its homolog SlCCD1A. Using in vitro assays performed with purified recombinant enzymes and by analyzing products formed by the two enzymes in carotene-accumulating Escherichia coli strains, we demonstrate that SlCCD1A and, to a larger extent, SlCCD1B, have a very relaxed specificity for both substrate and cleavage site, mediating the oxidative cleavage of cis- and all-trans-carotenoids as well as of different apocarotenoids at many more double bonds than previously reported. This activity gives rise to a plenitude of volatiles, mono-apocarotenoids and dialdehyde products, including cis-pseudoionone, neral, geranial, and farnesylacetone. Our results provide a direct evidence for a carotenoid origin of these compounds and point to CCD1s as the enzymes catalyzing the formation of the vast majority of tomato isoprenoid volatiles, many of which are aroma constituents. PMID:25057464

Strigolactone regulation of shoot branching in chrysanthemum (Dendranthema grandiflorum)

PubMed Central

Liang, Jianli; Zhao, Liangjun; Challis, Richard; Leyser, Ottoline

2010-01-01

Previous studies of highly branched mutants in pea (rms1–rms5), Arabidopsis thaliana (max1–max4), petunia (dad1–dad3), and rice (d3, d10, htd1/d17, d14, d27) identified strigolactones or their derivates (SLs), as shoot branching inhibitors. This recent discovery offers the possibility of using SLs to regulate branching commercially, for example, in chrysanthemum, an important cut flower crop. To investigate this option, SL physiology and molecular biology were studied in chrysanthemum (Dendranthema grandiflorum), focusing on the CCD8/MAX4/DAD1/RMS1/D10 gene. Our results suggest that, as has been proposed for Arabidopsis, the ability of SLs to inhibit bud activity depends on the presence of a competing auxin source. The chrysanthemum SL biosynthesis gene, CCD8 was cloned, and found to be regulated in a similar, but not identical way to known CCD8s. Expression analyses revealed that DgCCD8 is predominantly expressed in roots and stems, and is up-regulated by exogenous auxin. Exogenous SL can down-regulate DgCCD8 expression, but this effect can be overridden by apical auxin application. This study provides evidence that SLs are promising candidates to alter the shoot branching habit of chrysanthemum. PMID:20478970

Real-time monitoring of disintegration activity of catalytic core domain of HIV-1 integrase using molecular beacon.

PubMed

Zhang, Da-wei; Zhao, Ming-ming; He, Hong-qiu; Guo, Shun-xing

2013-09-15

HIV-1 integrase, an essential enzyme for retroviral replication, is a validated target for anti-HIV therapy development. The catalytic core domain of integrase (IN-CCD) is capable of catalyzing disintegration reaction. In this work, a hairpin-shaped disintegration substrate was designed and validated by enzyme-linked immunosorbent assay; a molecular beacon-based assay was developed for disintegration reaction of IN-CCD. Results showed that the disintegration substrate could be recognized and catalyzed by IN-CCD, and the disintegration reaction can be monitored according to the increase of fluorescent signal. The assay can be applied to real-time detection of disintegration with advantages of simplicity, high sensitivity, and excellent specificity. Copyright © 2013 Elsevier Inc. All rights reserved.

The superiority of L3-CCDs in the high-flux and wide dynamic range regimes

NASA Astrophysics Data System (ADS)

Butler, Raymond F.; Sheehan, Brendan J.

2008-02-01

Low Light Level CCD (L3-CCD) cameras have received much attention for high cadence astronomical imaging applications. Efforts to date have concentrated on exploiting them for two scenarios: post-exposure image sharpening and ``lucky imaging'', and rapid variability in astrophysically interesting sources. We demonstrate their marked superiority in a third distinct scenario: observing in the high-flux and wide dynamic range regimes. We realized that the unique features of L3-CCDs would make them ideal for maximizing signal-to-noise in observations of bright objects (whether variable or not), and for high dynamic range scenarios such as faint targets embedded in a crowded field of bright objects. Conventional CCDs have drawbacks in such regimes, due to a poor duty cycle-the combination of short exposure times (for time-series sampling or to avoid saturation) and extended readout times (for minimizing readout noise). For different telescope sizes, we use detailed models to show that a range of conventional imaging systems are photometrically out-performed across a wide range of object brightness, once the operational parameters of the L3-CCD are carefully set. The cross-over fluxes, above which the L3-CCD is operationally superior, are surprisingly faint-even for modest telescope apertures. We also show that the use of L3-CCDs is the optimum strategy for minimizing atmospheric scintillation noise in photometric observations employing a given telescope aperture. This is particularly significant, since scintillation can be the largest source of error in timeseries photometry. These results should prompt a new direction in developing imaging instrumentation solutions for observatories.

Construction of High-Quality Camel Immune Antibody Libraries.

PubMed

Romão, Ema; Poignavent, Vianney; Vincke, Cécile; Ritzenthaler, Christophe; Muyldermans, Serge; Monsion, Baptiste

2018-01-01

Single-domain antibodies libraries of heavy-chain only immunoglobulins from camelids or shark are enriched for high-affinity antigen-specific binders by a short in vivo immunization. Thus, potent binders are readily retrieved from relatively small-sized libraries of 10 7 -10 8 individual transformants, mostly after phage display and panning on a purified target. However, the remaining drawback of this strategy arises from the need to generate a dedicated library, for nearly every envisaged target. Therefore, all the procedures that shorten and facilitate the construction of an immune library of best possible quality are definitely a step forward. In this chapter, we provide the protocol to generate a high-quality immune VHH library using the Golden Gate Cloning strategy employing an adapted phage display vector where a lethal ccdB gene has to be substituted by the VHH gene. With this procedure, the construction of the library can be shortened to less than a week starting from bleeding the animal. Our libraries exceed 10 8 individual transformants and close to 100% of the clones harbor a phage display vector having an insert with the length of a VHH gene. These libraries are also more economic to make than previous standard approaches using classical restriction enzymes and ligations. The quality of the Nanobodies that are retrieved from immune libraries obtained by Golden Gate Cloning is identical to those from immune libraries made according to the classical procedure.

Curved CCD detector devices and arrays for multispectral astrophysical applications and terrestrial stereo panoramic cameras

NASA Astrophysics Data System (ADS)

Swain, Pradyumna; Mark, David

2004-09-01

The emergence of curved CCD detectors as individual devices or as contoured mosaics assembled to match the curved focal planes of astronomical telescopes and terrestrial stereo panoramic cameras represents a major optical design advancement that greatly enhances the scientific potential of such instruments. In altering the primary detection surface within the telescope"s optical instrumentation system from flat to curved, and conforming the applied CCD"s shape precisely to the contour of the telescope"s curved focal plane, a major increase in the amount of transmittable light at various wavelengths through the system is achieved. This in turn enables multi-spectral ultra-sensitive imaging with much greater spatial resolution necessary for large and very large telescope applications, including those involving infrared image acquisition and spectroscopy, conducted over very wide fields of view. For earth-based and space-borne optical telescopes, the advent of curved CCD"s as the principle detectors provides a simplification of the telescope"s adjoining optics, reducing the number of optical elements and the occurrence of optical aberrations associated with large corrective optics used to conform to flat detectors. New astronomical experiments may be devised in the presence of curved CCD applications, in conjunction with large format cameras and curved mosaics, including three dimensional imaging spectroscopy conducted over multiple wavelengths simultaneously, wide field real-time stereoscopic tracking of remote objects within the solar system at high resolution, and deep field survey mapping of distant objects such as galaxies with much greater multi-band spatial precision over larger sky regions. Terrestrial stereo panoramic cameras equipped with arrays of curved CCD"s joined with associative wide field optics will require less optical glass and no mechanically moving parts to maintain continuous proper stereo convergence over wider perspective viewing fields than their flat CCD counterparts, lightening the cameras and enabling faster scanning and 3D integration of objects moving within a planetary terrain environment. Preliminary experiments conducted at the Sarnoff Corporation indicate the feasibility of curved CCD imagers with acceptable electro-optic integrity. Currently, we are in the process of evaluating the electro-optic performance of a curved wafer scale CCD imager. Detailed ray trace modeling and experimental electro-optical data performance obtained from the curved imager will be presented at the conference.

Comparison of a CCD and an APS for soft X-ray diffraction

NASA Astrophysics Data System (ADS)

Stewart, Graeme; Bates, R.; Blue, A.; Clark, A.; Dhesi, S. S.; Maneuski, D.; Marchal, J.; Steadman, P.; Tartoni, N.; Turchetta, R.

2011-12-01

We compare a new CMOS Active Pixel Sensor (APS) to a Princeton Instruments PIXIS-XO: 2048B Charge Coupled Device (CCD) with soft X-rays tested in a synchrotron beam line at the Diamond Light Source (DLS). Despite CCDs being established in the field of scientific imaging, APS are an innovative technology that offers advantages over CCDs. These include faster readout, higher operational temperature, in-pixel electronics for advanced image processing and reduced manufacturing cost. The APS employed was the Vanilla sensor designed by the MI3 collaboration and funded by an RCUK Basic technology grant. This sensor has 520 x 520 square pixels, of size 25 μm on each side. The sensor can operate at a full frame readout of up to 20 Hz. The sensor had been back-thinned, to the epitaxial layer. This was the first time that a back-thinned APS had been demonstrated at a beam line at DLS. In the synchrotron experiment soft X-rays with an energy of approximately 708 eV were used to produce a diffraction pattern from a permalloy sample. The pattern was imaged at a range of integration times with both sensors. The CCD had to be operated at a temperature of -55°C whereas the Vanilla was operated over a temperature range from 20°C to -10°C. We show that the APS detector can operate with frame rates up to two hundred times faster than the CCD, without excessive degradation of image quality. The signal to noise of the APS is shown to be the same as that of the CCD at identical integration times and the response is shown to be linear, with no charge blooming effects. The experiment has allowed a direct comparison of back thinned APS and CCDs in a real soft x-ray synchrotron experiment.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lynch, Bart D.; Kozelka, Jakub; Ranade, Manisha K.

In this study, we present three significant artifacts that have the potential to negatively impact the accuracy and precision of film dosimetry measurements made using GAFCHROMIC[reg] EBT radiochromic film when read out with CCD flatbed scanners. Films were scanned using three commonly employed instruments: a Macbeth TD932 spot densitometer, an Epson Expression 1680 CCD array scanner, and a Microtek ScanMaker i900 CCD array scanner. For the two scanners we assessed the variation in optical density (OD) of GAFCHROMIC EBT film with scanning bed position, angular rotation of the film with respect to the scan line direction, and temperature inside themore » scanner due to repeated scanning. Scanning uniform radiochromic films demonstrated a distinct bowing effect in profiles in the direction of the CCD array with a nonuniformity of up to 17%. Profiles along a direction orthogonal to the CCD array demonstrated a 7% variation. A strong angular dependence was found in measurements made with the flatbed scanners; the effect could not be reproduced with the spot densitometer. An IMRT quality assurance film was scanned twice rotating the film 90 deg. between the scans. For films scanned on the Epson scanner, up to 12% variation was observed in unirradiated EBT films rotated between 0 deg. and 90 deg. , which decreased to approximately 8% for EBT films irradiated to 300 cGy. Variations of up to 80% were observed for films scanned with the Microtek scanner. The scanners were found to significantly increase the film temperature with repeated scanning. Film temperature between 18 and 33 deg. C caused OD changes of approximately 7%. Considering these effects, we recommend adherence to a strict scanning protocol that includes: maintaining the orientation of films scanned on flatbed scanners, limiting scanning to the central portion of the scanner bed, and limiting the number of consecutive scans to minimize changes in OD caused by film heating.« less

Public Elementary and Secondary School Student Enrollment, High School Completions, and Staff from the Common Core of Data: School Year 2005-06. First Look. NCES 2007-352

ERIC Educational Resources Information Center

Sable, Jennifer; Garofano, Anthony

2007-01-01

The Common Core of Data (CCD) is an annual universe collection of public elementary and secondary education data that is administered by the National Center for Education Statistics (NCES) and its data collection agent, the U.S. Census Bureau. Data for the CCD surveys are provided by state education agencies (SEAs). This report presents findings…

PN-CCD camera for XMM: performance of high time resolution/bright source operating modes

NASA Astrophysics Data System (ADS)

Kendziorra, Eckhard; Bihler, Edgar; Grubmiller, Willy; Kretschmar, Baerbel; Kuster, Markus; Pflueger, Bernhard; Staubert, Ruediger; Braeuninger, Heinrich W.; Briel, Ulrich G.; Meidinger, Norbert; Pfeffermann, Elmar; Reppin, Claus; Stoetter, Diana; Strueder, Lothar; Holl, Peter; Kemmer, Josef; Soltau, Heike; von Zanthier, Christoph

1997-10-01

The pn-CCD camera is developed as one of the focal plane instruments for the European photon imaging camera (EPIC) on board the x-ray multi mirror (XMM) mission to be launched in 1999. The detector consists of four quadrants of three pn-CCDs each, which are integrated on one silicon wafer. Each CCD has 200 by 64 pixels (150 micrometer by 150 micrometers) with 280 micrometers depletion depth. One CCD of a quadrant is read out at a time, while the four quadrants can be processed independently of each other. In standard imaging mode the CCDs are read out sequentially every 70 ms. Observations of point sources brighter than 1 mCrab will be effected by photon pile- up. However, special operating modes can be used to observe bright sources up to 150 mCrab in timing mode with 30 microseconds time resolution and very bright sources up to several crab in burst mode with 7 microseconds time resolution. We have tested one quadrant of the EPIC pn-CCD camera at line energies from 0.52 keV to 17.4 keV at the long beam test facility Panter in the focus of the qualification mirror module for XMM. In order to test the time resolution of the system, a mechanical chopper was used to periodically modulate the beam intensity. Pulse periods down to 0.7 ms were generated. This paper describes the performance of the pn-CCD detector in timing and burst readout modes with special emphasis on energy and time resolution.

Ion profiling in an ambient drift tube-ion mobility spectrometer using a high pixel density linear array detector IonCCD.

PubMed

Davila, Stephen J; Hadjar, Omar; Eiceman, Gary A

2013-07-16

A linear pixel-based detector array, the IonCCD, is characterized for use under ambient conditions with thermal (<1 eV) positive ions derived from purified air and a 10 mCi (63)Ni foil. The IonCCD combined with a drift tube-ion mobility spectrometer permitted the direct detection of gas phase ions at atmospheric pressure and confirmed a limit of detection of 3000 ions/pixel/frame established previously in both the keV (1-2 keV) and the hyper-thermal (10-40 eV) regimes. Results demonstrate the "broad-band" application of the IonCCD over 10(5) orders in ion energy and over 10(10) in operating pressure. The Faraday detector of a drift tube for an ion mobility spectrometer was replaced with the IonCCD providing images of ion profiles over the cross-section of the drift tube. Patterns in the ion profiles were developed in the drift tube cross-section by control of electric fields between wires of Bradbury Nielson and Tyndall Powell shutter designs at distances of 1-8 cm from the detector. Results showed that ion beams formed in wire sets, retained their shape with limited mixing by diffusion and Coulombic repulsion. Beam broadening determined as 95 μm/cm for hydrated protons in air with moisture of ~10 ppmv. These findings suggest a value of the IonCCD in further studies of ion motion and diffusion of thermalized ions, enhancing computational results from simulation programs, and in the design or operation of ion mobility spectrometers.

Diagnostics of underwater electrical wire explosion through a time- and space-resolved hard x-ray source

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sheftman, D.; Shafer, D.; Efimov, S.

2012-10-15

A time- and space-resolved hard x-ray source was developed as a diagnostic tool for imaging underwater exploding wires. A {approx}4 ns width pulse of hard x-rays with energies of up to 100 keV was obtained from the discharge in a vacuum diode consisting of point-shaped tungsten electrodes. To improve contrast and image quality, an external pulsed magnetic field produced by Helmholtz coils was used. High resolution x-ray images of an underwater exploding wire were obtained using a sensitive x-ray CCD detector, and were compared to optical fast framing images. Future developments and application of this diagnostic technique are discussed.

Design on the x-ray oral digital image display card

NASA Astrophysics Data System (ADS)

Wang, Liping; Gu, Guohua; Chen, Qian

2009-10-01

According to the main characteristics of X-ray imaging, the X-ray display card is successfully designed and debugged using the basic principle of correlated double sampling (CDS) and combined with embedded computer technology. CCD sensor drive circuit and the corresponding procedures have been designed. Filtering and sampling hold circuit have been designed. The data exchange with PC104 bus has been implemented. Using complex programmable logic device as a device to provide gating and timing logic, the functions which counting, reading CPU control instructions, corresponding exposure and controlling sample-and-hold have been completed. According to the image effect and noise analysis, the circuit components have been adjusted. And high-quality images have been obtained.

Diagnostics of underwater electrical wire explosion through a time- and space-resolved hard x-ray source.

PubMed

Sheftman, D; Shafer, D; Efimov, S; Gruzinsky, K; Gleizer, S; Krasik, Ya E

2012-10-01

A time- and space-resolved hard x-ray source was developed as a diagnostic tool for imaging underwater exploding wires. A ~4 ns width pulse of hard x-rays with energies of up to 100 keV was obtained from the discharge in a vacuum diode consisting of point-shaped tungsten electrodes. To improve contrast and image quality, an external pulsed magnetic field produced by Helmholtz coils was used. High resolution x-ray images of an underwater exploding wire were obtained using a sensitive x-ray CCD detector, and were compared to optical fast framing images. Future developments and application of this diagnostic technique are discussed.

Jig Aligns Shadow Mask On CCD

NASA Technical Reports Server (NTRS)

Matus, Carlos V.

1989-01-01

Alignment viewed through microscope. Alignment jig positions shadow mask on charge-coupled device (CCD) so metal film deposited on it precisely. Allows CCD package to be inserted and removed without disturbing alignment of mask. Holds CCD packages securely and isolates it electrostatically while providing electrical contact to each of its pins. When alignment jig assembled with CCD, used to move mask under micrometer control.

Cytosolic and Plastoglobule-targeted Carotenoid Dioxygenases from Crocus sativus Are Both Involved in β-Ionone Release*

PubMed Central

Rubio, Angela; Rambla, José Luís; Santaella, Marcella; Gómez, M. Dolores; Orzaez, Diego; Granell, Antonio; Gómez-Gómez, Lourdes

2008-01-01

Saffron, the processed stigma of Crocus sativus, is characterized by the presence of several apocarotenoids that contribute to the color, flavor, and aroma of the spice. However, little is known about the synthesis of aroma compounds during the development of the C. sativus stigma. The developing stigma is nearly odorless, but before and at anthesis, the aromatic compound β-ionone becomes the principal norisoprenoid volatile in the stigma. In this study, four carotenoid cleavage dioxygenase (CCD) genes, CsCCD1a, CsCCD1b, CsCCD4a, and CsCCD4b, were isolated from C. sativus. Expression analysis showed that CsCCD1a was constitutively expressed, CsCCD1b was unique to the stigma tissue, but only CsCCD4a and -b had expression patterns consistent with the highest levels of β-carotene and emission of β-ionone derived during the stigma development. The CsCCD4 enzymes were localized in plastids and more specifically were present in the plastoglobules. The enzymatic activities of CsCCD1a, CsCCD1b, and CsCCD4 enzymes were determined by Escherichia coli expression, and subsequent analysis of the volatile products was generated by GC/MS. The four CCDs fell in two phylogenetically divergent dioxygenase classes, but all could cleave β-carotene at the 9,10(9′,10′) positions to yield β-ionone. The data obtained suggest that all four C. sativus CCD enzymes may contribute in different ways to the production of β-ionone. In addition, the location and precise timing of β-ionone synthesis, together with its known activity as a fragrance and insect attractant, suggest that this volatile may have a role in Crocus pollination. PMID:18611853

Blinded evaluation of the effects of high definition and magnification on perceived image quality in laryngeal imaging.

PubMed

Otto, Kristen J; Hapner, Edie R; Baker, Michael; Johns, Michael M

2006-02-01

Advances in commercial video technology have improved office-based laryngeal imaging. This study investigates the perceived image quality of a true high-definition (HD) video camera and the effect of magnification on laryngeal videostroboscopy. We performed a prospective, dual-armed, single-blinded analysis of a standard laryngeal videostroboscopic examination comparing 3 separate add-on camera systems: a 1-chip charge-coupled device (CCD) camera, a 3-chip CCD camera, and a true 720p (progressive scan) HD camera. Displayed images were controlled for magnification and image size (20-inch [50-cm] display, red-green-blue, and S-video cable for 1-chip and 3-chip cameras; digital visual interface cable and HD monitor for HD camera). Ten blinded observers were then asked to rate the following 5 items on a 0-to-100 visual analog scale: resolution, color, ability to see vocal fold vibration, sense of depth perception, and clarity of blood vessels. Eight unblinded observers were then asked to rate the difference in perceived resolution and clarity of laryngeal examination images when displayed on a 10-inch (25-cm) monitor versus a 42-inch (105-cm) monitor. A visual analog scale was used. These monitors were controlled for actual resolution capacity. For each item evaluated, randomized block design analysis demonstrated that the 3-chip camera scored significantly better than the 1-chip camera (p < .05). For the categories of color and blood vessel discrimination, the 3-chip camera scored significantly better than the HD camera (p < .05). For magnification alone, observers rated the 42-inch monitor statistically better than the 10-inch monitor. The expense of new medical technology must be judged against its added value. This study suggests that HD laryngeal imaging may not add significant value over currently available video systems, in perceived image quality, when a small monitor is used. Although differences in clarity between standard and HD cameras may not be readily apparent on small displays, a large display size coupled with HD technology may impart improved diagnosis of subtle vocal fold lesions and vibratory anomalies.

The Extended Range X-Ray Telescope center director's discretionary fund report

NASA Technical Reports Server (NTRS)

Hoover, R. B.; Cumings, N. P.; Hildner, E.; Moore, R. L.; Tandberg-Hanssen, E. A.

1985-01-01

An Extended Range X-Ray Telescope (ERXRT) of high sensitivity and spatial resolution capable of functioning over a broad region of the X-ray/XUV portion of the spectrum has been designed and analyzed. This system has been configured around the glancing-incidence Wolter Type I X-ray mirror system which was flown on the Skylab Apollo Telescope Mount as ATM Experiment S-056. Enhanced sensitivity over a vastly broader spectral range can be realized by the utilization of a thinned, back-illuminated, buried-channel Charge Coupled Device (CCD) as the X-ray/XUV detector rather than photographic film. However, to maintain the high spatial resolution inherent in the X-ray optics when a CCD of 30 micron pixel size is used, it is necessary to increase the telescope plate scale. This can be accomplished by use of a glancing-incidence X-ray microscope to enlarge and re-focus the primary image onto the focal surface of the CCD.

cAMP-dependent chloride secretion mediates tubule enlargement and cyst formation by cultured mammalian collecting duct cells.

PubMed

Montesano, Roberto; Ghzili, Hafida; Carrozzino, Fabio; Rossier, Bernard C; Féraille, Eric

2009-02-01

Polycystic kidney diseases result from disruption of the genetically defined program that controls the size and geometry of renal tubules. Cysts which frequently arise from the collecting duct (CD) result from cell proliferation and fluid secretion. From mCCD(cl1) cells, a differentiated mouse CD cell line, we isolated a clonal subpopulation (mCCD-N21) that retains morphogenetic capacity. When grown in three-dimensional gels, mCCD-N21 cells formed highly organized tubular structures consisting of a palisade of polarized epithelial cells surrounding a cylindrical lumen. Subsequent addition of cAMP-elevating agents (forskolin or cholera toxin) or of membrane-permeable cAMP analogs (CPT-cAMP) resulted in rapid and progressive dilatation of existing tubules, leading to the formation of cystlike structures. When grown on filters, mCCD-N21 cells exhibited a high transepithelial resistance as well as aldosterone- and/or vasopressin-induced amiloride-sensitive and -insensitive current. The latter was in part inhibited by Na(+)-K(+)-2Cl(-) cotransporter (bumetanide) and chloride channel (NPPB) inhibitors. Real-time PCR analysis confirmed the expression of NKCC1, the ubiquitous Na(+)-K(+)-2Cl(-) cotransporter and cystic fibrosis transmembrane regulator (CFTR) in mCCD-N21 cells. Tubule enlargement and cyst formation were prevented by inhibitors of Na(+)-K(+)-2Cl(-) cotransporters (bumetanide or ethacrynic acid) or CFTR (NPPB or CFTR inhibitor-172). These results further support the notion that cAMP signaling plays a key role in renal cyst formation, at least in part by promoting chloride-driven fluid secretion. This new in vitro model of tubule-to-cyst conversion affords a unique opportunity for investigating the molecular mechanisms that govern the architecture of epithelial tubes, as well as for dissecting the pathophysiological processes underlying cystic kidney diseases.

Reaching the Diffraction Limit - Differential Speckle and Wide-Field Imaging for the WIYN Telescope

NASA Technical Reports Server (NTRS)

Scott, Nic J.; Howell, Steve; Horch, Elliott

2016-01-01

Speckle imaging allows telescopes to achieve diffraction limited imaging performance. The technique requires cameras capable of reading out frames at a very fast rate, effectively 'freezing out' atmospheric seeing. The resulting speckles can be correlated and images reconstructed that are at the diffraction limit of the telescope. These new instruments are based on the successful performance and design of the Differential Speckle Survey Instrument (DSSI).The instruments are being built for the Gemini-N and WIYN telescopes and will be made available to the community via the peer review proposal process. We envision their primary use to be validation and characterization of exoplanet targets from the NASA, K2 and TESS missions and RV discovered exoplanets. Such targets will provide excellent follow-up candidates for both the WIYN and Gemini telescopes. We expect similar data quality in speckle imaging mode with the new instruments. Additionally, both cameras will have a wide-field mode and standard SDSS filters. They will be highly versatile instruments and it is that likely many other science programs will request time on the cameras. The limiting magnitude for speckle observations will remain around 13-14th at WIYN and 16-17th at Gemini, while wide-field, normal CCD imaging operation should be able to go to much fainter, providing usual CCD imaging and photometric capabilities. The instruments will also have high utility as scoring cameras for telescope engineering purposes, or other applications where high time resolution is needed. Instrument support will be provided, including a software pipeline that takes raw speckle data to fully reconstructed images.

Real-time monitoring of laser welding of galvanized high strength steel in lap joint configuration

NASA Astrophysics Data System (ADS)

Kong, Fanrong; Ma, Junjie; Carlson, Blair; Kovacevic, Radovan

2012-10-01

Two different cases regarding the zinc coating at the lap joint faying surface are selected for studying the influence of zinc vapor on the keyhole dynamics of the weld pool and the final welding quality. One case has the zinc coating fully removed at the faying surface; while the other case retains the zinc coating on the faying surface. It is found that removal of the zinc coating at the faying surface produces a significantly better weld quality as exemplified by a lack of spatters whereas intense spatters are present when the zinc coating is present at the faying surface. Spectroscopy is used to detect the optical spectra emitted from a laser generated plasma plume during the laser welding of galvanized high strength DP980 steel in a lap-joint configuration. A correlation between the electron temperature and defects within the weld bead is identified by using the Boltzmann plot method. The laser weld pool keyhole dynamic behavior affected by a high-pressure zinc vapor generated at the faying surface of galvanized steel lap-joint is monitored in real-time by a high speed charge-coupled device (CCD) camera assisted with a green laser as an illumination source.

A strand-passage conformation of DNA gyrase is required to allow the bacterial toxin, CcdB, to access its binding site

PubMed Central

Smith, Andrew B.; Maxwell, Anthony

2006-01-01

DNA gyrase is the only topoisomerase able to introduce negative supercoils into DNA. Absent in humans, gyrase is a successful target for antibacterial drugs. However, increasing drug resistance is a serious problem and new agents are urgently needed. The naturally-produced Escherichia coli toxin CcdB has been shown to target gyrase by what is predicted to be a novel mechanism. CcdB has been previously shown to stabilize the gyrase ‘cleavage complex’, but it has not been shown to inhibit the catalytic reactions of gyrase. We present data showing that CcdB does indeed inhibit the catalytic reactions of gyrase by stabilization of the cleavage complex and that the GyrA C-terminal DNA-wrapping domain and the GyrB N-terminal ATPase domain are dispensable for CcdB's action. We further investigate the role of specific GyrA residues in the action of CcdB by site-directed mutagenesis; these data corroborate a model for CcdB action based on a recent crystal structure of a CcdB–GyrA fragment complex. From this work, we are now able to present a model for CcdB action that explains all previous observations relating to CcdB–gyrase interaction. CcdB action requires a conformation of gyrase that is only revealed when DNA strand passage is taking place. PMID:16963775

A strand-passage conformation of DNA gyrase is required to allow the bacterial toxin, CcdB, to access its binding site.

PubMed

Smith, Andrew B; Maxwell, Anthony

2006-01-01

DNA gyrase is the only topoisomerase able to introduce negative supercoils into DNA. Absent in humans, gyrase is a successful target for antibacterial drugs. However, increasing drug resistance is a serious problem and new agents are urgently needed. The naturally-produced Escherichia coli toxin CcdB has been shown to target gyrase by what is predicted to be a novel mechanism. CcdB has been previously shown to stabilize the gyrase 'cleavage complex', but it has not been shown to inhibit the catalytic reactions of gyrase. We present data showing that CcdB does indeed inhibit the catalytic reactions of gyrase by stabilization of the cleavage complex and that the GyrA C-terminal DNA-wrapping domain and the GyrB N-terminal ATPase domain are dispensable for CcdB's action. We further investigate the role of specific GyrA residues in the action of CcdB by site-directed mutagenesis; these data corroborate a model for CcdB action based on a recent crystal structure of a CcdB-GyrA fragment complex. From this work, we are now able to present a model for CcdB action that explains all previous observations relating to CcdB-gyrase interaction. CcdB action requires a conformation of gyrase that is only revealed when DNA strand passage is taking place.

Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE7 is involved in the production of negative and positive branching signals in petunia.

PubMed

Drummond, Revel S M; Martínez-Sánchez, N Marcela; Janssen, Bart J; Templeton, Kerry R; Simons, Joanne L; Quinn, Brian D; Karunairetnam, Sakuntala; Snowden, Kimberley C

2009-12-01

One of the key factors that defines plant form is the regulation of when and where branches develop. The diversity of form observed in nature results, in part, from variation in the regulation of branching between species. Two CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes, CCD7 and CCD8, are required for the production of a branch-suppressing plant hormone. Here, we report that the decreased apical dominance3 (dad3) mutant of petunia (Petunia hybrida) results from the mutation of the PhCCD7 gene and has a less severe branching phenotype than mutation of PhCCD8 (dad1). An analysis of the expression of this gene in wild-type, mutant, and grafted petunia suggests that in petunia, CCD7 and CCD8 are coordinately regulated. In contrast to observations in Arabidopsis (Arabidopsis thaliana), ccd7ccd8 double mutants in petunia show an additive phenotype. An analysis using dad3 or dad1 mutant scions grafted to wild-type rootstocks showed that when these plants produce adventitious mutant roots, branching is increased above that seen in plants where the mutant roots are removed. The results presented here indicate that mutation of either CCD7 or CCD8 in petunia results in both the loss of an inhibitor of branching and an increase in a promoter of branching.

Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE7 Is Involved in the Production of Negative and Positive Branching Signals in Petunia1[W][OA

PubMed Central

Drummond, Revel S.M.; Martínez-Sánchez, N. Marcela; Janssen, Bart J.; Templeton, Kerry R.; Simons, Joanne L.; Quinn, Brian D.; Karunairetnam, Sakuntala; Snowden, Kimberley C.

2009-01-01

One of the key factors that defines plant form is the regulation of when and where branches develop. The diversity of form observed in nature results, in part, from variation in the regulation of branching between species. Two CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes, CCD7 and CCD8, are required for the production of a branch-suppressing plant hormone. Here, we report that the decreased apical dominance3 (dad3) mutant of petunia (Petunia hybrida) results from the mutation of the PhCCD7 gene and has a less severe branching phenotype than mutation of PhCCD8 (dad1). An analysis of the expression of this gene in wild-type, mutant, and grafted petunia suggests that in petunia, CCD7 and CCD8 are coordinately regulated. In contrast to observations in Arabidopsis (Arabidopsis thaliana), ccd7ccd8 double mutants in petunia show an additive phenotype. An analysis using dad3 or dad1 mutant scions grafted to wild-type rootstocks showed that when these plants produce adventitious mutant roots, branching is increased above that seen in plants where the mutant roots are removed. The results presented here indicate that mutation of either CCD7 or CCD8 in petunia results in both the loss of an inhibitor of branching and an increase in a promoter of branching. PMID:19846541

The New Amateur Astronomer

NASA Astrophysics Data System (ADS)

Mobberley, Martin

Amateur astronomy has changed beyond recognition in less than two decades. The reason is, of course, technology. Affordable high-quality telescopes, computer-controlled 'go to' mountings, autoguiders, CCD cameras, video, and (as always) computers and the Internet, are just a few of the advances that have revolutionized astronomy for the twenty-first century. Martin Mobberley first looks at the basics before going into an in-depth study of what’s available commercially. He then moves on to the revolutionary possibilities that are open to amateurs, from imaging, through spectroscopy and photometry, to patrolling for near-earth objects - the search for comets and asteroids that may come close to, or even hit, the earth. The New Amateur Astronomer is a road map of the new astronomy, equally suitable for newcomers who want an introduction, or old hands who need to keep abreast of innovations. From the reviews: "This is one of several dozen books in Patrick Moore's "Practical Astronomy" series. Amid this large family, Mobberley finds his niche: the beginning high-tech amateur. The book's first half discusses equipment: computer-driven telescopes, CCD cameras, imaging processing software, etc. This market is changing every bit as rapidly as the computer world, so these details will be current for only a year or two. The rest of the book offers an overview of scientific projects that serious amateurs are carrying out these days. Throughout, basic formulas and technical terms are provided as needed, without formal derivations. An appendix with useful references and Web sites is also included. Readers will need more than this book if they are considering a plunge into high-tech amateur astronomy, but it certainly will whet their appetites. Mobberley's most valuable advice will save the book's owner many times its cover price: buy a quality telescope from a reputable dealer and install it in a simple shelter so it can be used with as little set-up time as possible. A poor purchase choice and the hassle of setting up are why most fancy telescopes gather dust in their owners' dens. Summing Up: Highly recommended. General readers; lower- and upper-division undergraduates."( T. D. Oswalt, CHOICE, March 2005)

Reduction of Cross-Reactive Carbohydrate Determinants in Plant Foodstuff: Elucidation of Clinical Relevance and Implications for Allergy Diagnosis

PubMed Central

Brehler, Randolf; von Schaewen, Antje

2011-01-01

Background A longstanding debate in allergy is whether or not specific immunoglobulin-E antibodies (sIgE), recognizing cross-reactive carbohydrate determinants (CCD), are able to elicit clinical symptoms. In pollen and food allergy, ≥20% of patients display in-vitro CCD reactivity based on presence of α1,3-fucose and/or β1,2-xylose residues on N-glycans of plant (xylose/fucose) and insect (fucose) glycoproteins. Because the allergenicity of tomato glycoallergen Lyc e 2 was ascribed to N-glycan chains alone, this study aimed at evaluating clinical relevance of CCD-reduced foodstuff in patients with carbohydrate-specific IgE (CCD-sIgE). Methodology/Principal Findings Tomato and/or potato plants with stable reduction of Lyc e 2 (tomato) or CCD formation in general were obtained via RNA interference, and gene-silencing was confirmed by immunoblot analyses. Two different CCD-positive patient groups were compared: one with tomato and/or potato food allergy and another with hymenoptera-venom allergy (the latter to distinguish between CCD- and peptide-specific reactions in the food-allergic group). Non-allergic and CCD-negative food-allergic patients served as controls for immunoblot, basophil activation, and ImmunoCAP analyses. Basophil activation tests (BAT) revealed that Lyc e 2 is no key player among other tomato (glyco)allergens. CCD-positive patients showed decreased (re)activity with CCD-reduced foodstuff, most obvious in the hymenoptera venom-allergic but less in the food-allergic group, suggesting that in-vivo reactivity is primarily based on peptide- and not CCD-sIgE. Peptide epitopes remained unaffected in CCD-reduced plants, because CCD-negative patient sera showed reactivity similar to wild-type. In-house-made ImmunoCAPs, applied to investigate feasibility in routine diagnosis, confirmed BAT results at the sIgE level. Conclusions/Significance CCD-positive hymenoptera venom-allergic patients (control group) showed basophil activation despite no allergic symptoms towards tomato and potato. Therefore, this proof-of-principle study demonstrates feasibility of CCD-reduced foodstuff to minimize ‘false-positive results’ in routine serum tests. Despite confirming low clinical relevance of CCD antibodies, we identified one patient with ambiguous in-vitro results, indicating need for further component-resolved diagnosis. PMID:21423762

Sun and Sky Radiance Measurements and Data Analysis Protocols. Chapter 5

NASA Technical Reports Server (NTRS)

Frouin, Robert; Holben, Brent; Miller, Mark; Pietras, Christophe; Porter, John; Voss, Ken

2001-01-01

This chapter is concerned with two types of radiometric measurements essential to verify atmospheric correction algorithms and to calibrate vicariously satellite ocean color sensors. The first type is a photometric measurement of the direct solar beam to determine the optical thickness of the atmosphere. The intensity of the solar beam can be measured directly, or obtained indirectly from measurements of diffuse global upper hemispheric irradiance. The second type is a measurement of the solar aureole and sky radiance distribution using a CCD camera, or a scanning radiometer viewing in and perpendicular to the solar principal plane. From the two types of measurements, the optical properties of aerosols, highly variable in space and time, can be derived. Because of the high variability, the aerosol properties should be known at the time of satellite overpass. Atmospheric optics measurements, however, are not easy to perform at sea, from a ship or any platform. This complicates the measurement protocols and data analysis. Some instrumentation cannot be deployed at sea, and is limited to island and coastal sites. In the following, measurement protocols are described for radiometers commonly used to measure direct atmospheric transmittance and sky radiance, namely standard sun photometers, fast-rotating shadow-band radiometers, automated sky scanning systems, and CCD cameras. Methods and procedures to analyze and quality control the data are discussed, as well as proper measurement strategies for evaluation of atmospheric correction algorithms and satellite-derived ocean color.

A curved surface micro-moiré method and its application in evaluating curved surface residual stress

NASA Astrophysics Data System (ADS)

Zhang, Hongye; Wu, Chenlong; Liu, Zhanwei; Xie, Huimin

2014-09-01

The moiré method is typically applied to the measurement of deformations of a flat surface while, for a curved surface, this method is rarely used other than for projection moiré or moiré interferometry. Here, a novel colour charge-coupled device (CCD) micro-moiré method has been developed, based on which a curved surface micro-moiré (CSMM) method is proposed with a colour CCD and optical microscope (OM). In the CSMM method, no additional reference grating is needed as a Bayer colour filter array (CFA) installed on the OM in front of the colour CCD image sensor performs this role. Micro-moiré fringes with high contrast are directly observed with the OM through the Bayer CFA under the special condition of observing a curved specimen grating. The principle of the CSMM method based on a colour CCD micro-moiré method and its application range and error analysis are all described in detail. In an experiment, the curved surface residual stress near a welded seam on a stainless steel tube was investigated using the CSMM method.

Intermittent carbonate sedimentation in the equatoral Indian Ocean: fluctuations of the Eocene CCD?

NASA Astrophysics Data System (ADS)

Mitchison, F.; Kachovich, S.; Backman, J.; Pike, J.

2017-12-01

IODP Expedition 362 recently drilled from the sea floor to oceanic basement in the eastern equatorial Indian Ocean at Site U1480G (3°N, 91°E, water depth 4148 m). Beneath the thick ( 1250 m) predominantly siliciclastic Nicobar Fan succession, a condensed ( 10 m) middle Eocene pelagic interval displayed striking decimetre-scale banding, alternating between calcareous oozes and darker clays. We investigate whether deposition of the calcareous sediments was associated with periodic global carbonate accumulation events previously documented in the Equatorial Pacific and Atlantic Oceans, linked to oscillations of the carbonate compensation depth (CCD). We present high-resolution geochemical records (carbonate, organic carbon, bulk carbonate stable isotopes) and scanning electron microscope micro-element maps through several of the calcareous to clay transitions, as well as microfossil assemblages and new biostratigraphic constraints for the interval. Our data will reveal whether the banded sediments represent fluctuations of the CCD, and whether the CCD was likely responding to global (e.g. changes in pCO2) or local (e.g. local changes in calcareous plankton productivity) processes.

A system design of data acquisition and processing for side-scatter lidar

NASA Astrophysics Data System (ADS)

Zhang, ZhanYe; Xie, ChenBo; Wang, ZhenZhu; Kuang, ZhiQiang; Deng, Qian; Tao, ZongMing; Liu, Dong; Wang, Yingjian

2018-03-01

A system for collecting data of Side-Scatter lidar based on Charge Coupled Device (CCD),is designed and implemented. The system of data acquisition is based on Microsoft. Net structure and the language of C# is used to call dynamic link library (DLL) of CCD for realization of the real-time data acquisition and processing. The software stores data as txt file for post data acquisition and analysis. The system has ability to operate CCD device in all-day, automatic, continuous and high frequency data acquisition and processing conditions, which will catch 24-hour information of the atmospheric scatter's light intensity and retrieve the spatial and temporal properties of aerosol particles. The experimental result shows that the system is convenient to observe the aerosol optical characteristics near surface.

Time-resolved spectra of dense plasma focus using spectrometer, streak camera, and CCD combination.

PubMed

Goldin, F J; Meehan, B T; Hagen, E C; Wilkins, P R

2010-10-01

A time-resolving spectrographic instrument has been assembled with the primary components of a spectrometer, image-converting streak camera, and CCD recording camera, for the primary purpose of diagnosing highly dynamic plasmas. A collection lens defines the sampled region and couples light from the plasma into a step index, multimode fiber which leads to the spectrometer. The output spectrum is focused onto the photocathode of the streak camera, the output of which is proximity-coupled to the CCD. The spectrometer configuration is essentially Czerny-Turner, but off-the-shelf Nikon refraction lenses, rather than mirrors, are used for practicality and flexibility. Only recently assembled, the instrument requires significant refinement, but has now taken data on both bridge wire and dense plasma focus experiments.

Breast Biopsy System

NASA Technical Reports Server (NTRS)

1994-01-01

Charge Coupled Devices (CCDs) are high technology silicon chips that connect light directly into electronic or digital images, which can be manipulated or enhanced by computers. When Goddard Space Flight Center (GSFC) scientists realized that existing CCD technology could not meet scientific requirements for the Hubble Space Telescope Imagining Spectrograph, GSFC contracted with Scientific Imaging Technologies, Inc. (SITe) to develop an advanced CCD. SITe then applied many of the NASA-driven enhancements to the manufacture of CCDs for digital mammography. The resulting device images breast tissue more clearly and efficiently. The LORAD Stereo Guide Breast Biopsy system incorporates SITe's CCD as part of a digital camera system that is replacing surgical biopsy in many cases. Known as stereotactic needle biopsy, it is performed under local anesthesia with a needle and saves women time, pain, scarring, radiation exposure and money.

High-speed line-scan camera with digital time delay integration

NASA Astrophysics Data System (ADS)

Bodenstorfer, Ernst; Fürtler, Johannes; Brodersen, Jörg; Mayer, Konrad J.; Eckel, Christian; Gravogl, Klaus; Nachtnebel, Herbert

2007-02-01

Dealing with high-speed image acquisition and processing systems, the speed of operation is often limited by the amount of available light, due to short exposure times. Therefore, high-speed applications often use line-scan cameras, based on charge-coupled device (CCD) sensors with time delayed integration (TDI). Synchronous shift and accumulation of photoelectric charges on the CCD chip - according to the objects' movement - result in a longer effective exposure time without introducing additional motion blur. This paper presents a high-speed color line-scan camera based on a commercial complementary metal oxide semiconductor (CMOS) area image sensor with a Bayer filter matrix and a field programmable gate array (FPGA). The camera implements a digital equivalent to the TDI effect exploited with CCD cameras. The proposed design benefits from the high frame rates of CMOS sensors and from the possibility of arbitrarily addressing the rows of the sensor's pixel array. For the digital TDI just a small number of rows are read out from the area sensor which are then shifted and accumulated according to the movement of the inspected objects. This paper gives a detailed description of the digital TDI algorithm implemented on the FPGA. Relevant aspects for the practical application are discussed and key features of the camera are listed.

ACS Data Handbook v.6.0

NASA Astrophysics Data System (ADS)

Gonzaga, S.; et al.

2011-03-01

ACS was designed to provide a deep, wide-field survey capability from the visible to near-IR using the Wide Field Camera (WFC), high resolution imaging from the near-UV to near-IR with the now-defunct High Resolution Camera (HRC), and solar-blind far-UV imaging using the Solar Blind Camera (SBC). The discovery efficiency of ACS's Wide Field Channel (i.e., the product of WFC's field of view and throughput) is 10 times greater than that of WFPC2. The failure of ACS's CCD electronics in January 2007 brought a temporary halt to CCD imaging until Servicing Mission 4 in May 2009, when WFC functionality was restored. Unfortunately, the high-resolution optical imaging capability of HRC was not recovered.

CCD Photometer Installed on the Telescope - 600 OF the Shamakhy Astrophysical Observatory II. The Technique of Observation and Data Processing of CCD Photometry

NASA Astrophysics Data System (ADS)

Abdullayev, B. I.; Gulmaliyev, N. I.; Majidova, S. O.; Mikayilov, Kh. M.; Rustamov, B. N.

2009-12-01

Basic technical characteristics of CCD matrix U-47 made by the Apogee Alta Instruments Inc. are provided. Short description and features of various noises introduced by optical system and CCD camera are presented. The technique of getting calibration frames: bias, dark, flat field and main stages of processing of results CCD photometry are described.

Pixel-based characterisation of CMOS high-speed camera systems

NASA Astrophysics Data System (ADS)

Weber, V.; Brübach, J.; Gordon, R. L.; Dreizler, A.

2011-05-01

Quantifying high-repetition rate laser diagnostic techniques for measuring scalars in turbulent combustion relies on a complete description of the relationship between detected photons and the signal produced by the detector. CMOS-chip based cameras are becoming an accepted tool for capturing high frame rate cinematographic sequences for laser-based techniques such as Particle Image Velocimetry (PIV) and Planar Laser Induced Fluorescence (PLIF) and can be used with thermographic phosphors to determine surface temperatures. At low repetition rates, imaging techniques have benefitted from significant developments in the quality of CCD-based camera systems, particularly with the uniformity of pixel response and minimal non-linearities in the photon-to-signal conversion. The state of the art in CMOS technology displays a significant number of technical aspects that must be accounted for before these detectors can be used for quantitative diagnostics. This paper addresses these issues.

Performance of the Digital Science Partnership Remotely-Operated 0.5-Meter Corrected Dall-Kirkham Telescopes

NASA Astrophysics Data System (ADS)

Kielkopf, John F.; Carter, B.; Brown, C.; Hart, R.; Hay, J.; Waite, I.

2007-12-01

The Digital Science Partnership, a collaboration of the University of Louisville and the University of Southern Queensland, operates a pair of 0.5-meter telescopes for teaching, research, and informal education. The instruments were installed at sites near Toowoomba, Australia, and Louisville, Kentucky in 2006. The Planewave Instruments optical systems employ a unique Dall-Kirkham design incorporating a two-element corrector that demagnifies the image, flattens the focal plane, and reduces coma. These instruments have a moderately fast f/6.8 focal ratio and maintain image quality with little vignetting over a field 42 mm in diameter (0.7 degree). With a 9-micron pixel CCD such as the KAF-6303E, the image scale of 0.55 seconds of arc per pixel typically yields seeing-limited image quality at our sites. The telescopes and their enclosure are operated in a live remote observing mode through Linux-based software, including a dome-control system that uses RFID tags for absolute rotation encoding. After several months of testing and development we have examples of images and photometry from both sites that illustrate the performance of the system. We will discuss image quality, as well as practical matters such as pointing accuracy and field acquisition, auto-guiding, communication latency in large file transfer, and our experience with remote observing assisted by teleconferencing. Time-delay-integration (TDI) imaging, in which the telescope is stationary while the CCD is clocked to track in right ascension, is under study. The technique offers wide fields of view with very high signal-to-noise ratio, and can be implemented in robotically operated instruments used in monitoring, rapid-response, and educational programs. Results for conventional and TDI imaging from the dark site in Australia compared to the brighter suburban site in Kentucky show the benefits of access to dark sites through international partnerships that remote operation technology offers.

CCD TV focal plane guider development and comparison to SIRTF applications

NASA Technical Reports Server (NTRS)

Rank, David M.

1989-01-01

It is expected that the SIRTF payload will use a CCD TV focal plane fine guidance sensor to provide acquisition of sources and tracking stability of the telescope. Work has been done to develop CCD TV cameras and guiders at Lick Observatory for several years and have produced state of the art CCD TV systems for internal use. NASA decided to provide additional support so that the limits of this technology could be established and a comparison between SIRTF requirements and practical systems could be put on a more quantitative basis. The results of work carried out at Lick Observatory which was designed to characterize present CCD autoguiding technology and relate it to SIRTF applications is presented. Two different design types of CCD cameras were constructed using virtual phase and burred channel CCD sensors. A simple autoguider was built and used on the KAO, Mt. Lemon and Mt. Hamilton telescopes. A video image processing system was also constructed in order to characterize the performance of the auto guider and CCD cameras.

Applications of a pnCCD detector coupled to columnar structure CsI(Tl) scintillator system in ultra high energy X-ray Laue diffraction

NASA Astrophysics Data System (ADS)

Shokr, M.; Schlosser, D.; Abboud, A.; Algashi, A.; Tosson, A.; Conka, T.; Hartmann, R.; Klaus, M.; Genzel, C.; Strüder, L.; Pietsch, U.

2017-12-01

Most charge coupled devices (CCDs) are made of silicon (Si) with typical active layer thicknesses of several microns. In case of a pnCCD detector the sensitive Si thickness is 450 μm. However, for silicon based detectors the quantum efficiency for hard X-rays drops significantly for photon energies above 10 keV . This drawback can be overcome by combining a pixelated silicon-based detector system with a columnar scintillator. Here we report on the characterization of a low noise, fully depleted 128×128 pixels pnCCD detector with 75×75 μm2 pixel size coupled to a 700 μm thick columnar CsI(Tl) scintillator in the photon range between 1 keV to 130 keV . The excellent performance of the detection system in the hard X-ray range is demonstrated in a Laue type X-ray diffraction experiment performed at EDDI beamline of the BESSY II synchrotron taken at a set of several GaAs single crystals irradiated by white synchrotron radiation. With the columnar structure of the scintillator, the position resolution of the whole system reaches a value of less than one pixel. Using the presented detector system and considering the functional relation between indirect and direct photon events Laue diffraction peaks with X-ray energies up to 120 keV were efficiently detected. As one of possible applications of the combined CsI-pnCCD system we demonstrate that the accuracy of X-ray structure factors extracted from Laue diffraction peaks can be significantly improved in hard X-ray range using the combined CsI(Tl)-pnCCD system compared to a bare pnCCD.

A metagenomic survey of microbes in honey bee colony collapse disorder.

PubMed

Cox-Foster, Diana L; Conlan, Sean; Holmes, Edward C; Palacios, Gustavo; Evans, Jay D; Moran, Nancy A; Quan, Phenix-Lan; Briese, Thomas; Hornig, Mady; Geiser, David M; Martinson, Vince; vanEngelsdorp, Dennis; Kalkstein, Abby L; Drysdale, Andrew; Hui, Jeffrey; Zhai, Junhui; Cui, Liwang; Hutchison, Stephen K; Simons, Jan Fredrik; Egholm, Michael; Pettis, Jeffery S; Lipkin, W Ian

2007-10-12

In colony collapse disorder (CCD), honey bee colonies inexplicably lose their workers. CCD has resulted in a loss of 50 to 90% of colonies in beekeeping operations across the United States. The observation that irradiated combs from affected colonies can be repopulated with naive bees suggests that infection may contribute to CCD. We used an unbiased metagenomic approach to survey microflora in CCD hives, normal hives, and imported royal jelly. Candidate pathogens were screened for significance of association with CCD by the examination of samples collected from several sites over a period of 3 years. One organism, Israeli acute paralysis virus of bees, was strongly correlated with CCD.

Silicide Schottky Barrier For Back-Surface-Illuminated CCD

NASA Technical Reports Server (NTRS)

Hecht, Michael H.

1990-01-01

Quantum efficiency of back-surface-illuminated charge-coupled device (CCD) increased by coating back surface with thin layer of PtSi or IrSi on thin layer of SiO2. In its interaction with positively-doped bulk Si of CCD, silicide/oxide layer forms Schottky barrier that repels electrons, promoting accumulation of photogenerated charge carriers in front-side CCD potential wells. Physical principle responsible for improvement explained in "Metal Film Increases CCD Output" (NPO-16815).

Eventual Participation in GMES of Institute of Geodesy and Geoinformation

NASA Astrophysics Data System (ADS)

Balodis, J.; Janpaule, I.; Rubans, A.; Zarinjsh, A.; Abele, M.; Ubelis, A.; Cekule, M.

2012-04-01

The new project has been commenced at the University of Latvia (LU) - "Fotonika-LV". Three institutes, namely institute of Atomphysics and Spectroscopy, Institute of Astronomy and Institute of Geodesy and Geoinformation have succeeded to receive the sources for proposed development of photonics in applied research. Several highly advanced partner institutions have agreed to establish partnership in planned research activities. The photonics plays an important role at the R&D of the Institute of Geodesy and Geoinformation of the University of Latvia (LU GGI). The Institute applies the space related technologies for the environmental studies in Latvia. Photonics has been applied in satellite laser ranging systems. The small size modern satellite laser ranging system (SLR) and its control software has been developed at the Institute recently. SLR will be used for the regular observations of low Earth orbiters (LAGEOS, GOCE, GRACE, ERS2, ENVISAT, CRYOSAT, etc.) within the framework of ILRS. The test observations have proved the results of high quality. Sentinel mission satellites could be observed as well if they will have the laser retroreflectors. The developed SLR is a small size. It could be improved for the mobility applications in variety of sites if needed. Another SLR machine is under construction with planned application for remote sensing satellite calibration by using the white laser beam. Additionally it could be used for Galileo and LAGEOS observation. However, in order to use the SLR for Galileo and other higher orbit satellites the most sensitive photonics are needed. From other side the proper optical devices are needed for SLR observations at the daylight. Additionally, the sky in the Baltic region is frequently covered by the thin clouds which make the satellite observations very complicated and less productive. The optimal choice of photonics in each case is needed and best solutions are required. The CCD matrices combination with especially made optical devices has been used in SLR system for the visual guidance of the satellite tracking. The R&D in CCD matrices construction and application technologies are developed with increased variety in several countries. The application of advanced CCD techniques in SLR systems is a very powerful tool. To follow the CCD techniques development race and to apply photonics achievements in various devices of geodetic techniques is very important for the Institute. Also development of the mobile zenith camera for determination of vertical deflection is being carried out. Zenith camera will serve for the studies of anomalies of regional gravitation field. The observation procedure is based on the analysis of the stellar sky imageries obtained on the CCD matrices similarly like in star tracker. Both the resolution quality and the sensitivity of the matrices are the key elements for high accuracy vertical deflection determination. Application of Global Navigation Satellite Systems (GNSS) in geodesy discovers a powerful tool for the verification and validation of the height values of geodetic levelling benchmarks established historically long time ago. The differential GNSS and RTK methods appear very useful to identify the vertical displacement of landscape by means of inspection of the deformation of levelling networks. Within the European framework of ground based GNSS European positioning augmentation system EUPOS® the local EUPOS®-Riga continuously operating geodetic reference system has been developed by LU GGI in co-operation with Rigas GeoMetrs land surveying company. The system consists of 5 GNSS base station network located within the framework of Riga city. The system has been properly investigated and controlled. The GNSS observation RTCM corrections produced by the EUPOS®-Riga system can be used for high precision position determination in various navigation and land surveying applications. All the developments carried out at the Institute serve for the studies and for the monitoring of the environmental changes. The satellite imagery maps are used too. The knowledge of photonics discovers additional capabilities in development of airborne and spaceborn applications for earth observation. The ongoing process is accelerated in co-operation with a Riga Technical University. Scientific staff of "Fotonika-LV" project and LU GGI are looking forward for eventual participation in GMES project.

Time-resolved imaging of the plasma development in a triggered vacuum switch

NASA Astrophysics Data System (ADS)

Park, Wung-Hoa; Kim, Moo-Sang; Son, Yoon-Kyoo; Frank, Klaus; Lee, Byung-Joon; Ackerman, Thilo; Iberler, Marcus

2017-12-01

Triggered vacuum switches (TVS) are particularly used in pulsed power technology as closing switches for high voltages and high charge transfer. A non-sealed-off prototype was designed with a side-on quartz window to investigate the evolution of the trigger discharge into the main discharge. The image acquisition was done with a fast CCD camera PI-MAX2 from Princeton Instruments. The CCD camera has a maximum exposure time of 2 ns. The electrode configuration of the prototype is a conventional six-rod gap type, a capacitor bank with C = 16.63 μF, which corresponds at 20 kV charging voltage to a total stored charge of 0.3 C or a total energy of 3.3 kJ. The peak current is 88 kA. According to the tremendously highly different light intensities during the trigger and main discharge, the complete discharge is split into three phases: a trigger breakdown phase, an intermediate phase and a main discharge phase. The CCD camera images of the first phase show instabilities of the trigger breakdown, in phase 2 three different discharge modes are observed. After the first current maximum the discharge behavior is reproducible.

Smear correction of highly variable, frame-transfer CCD images with application to polarimetry.

PubMed

Iglesias, Francisco A; Feller, Alex; Nagaraju, Krishnappa

2015-07-01

Image smear, produced by the shutterless operation of frame-transfer CCD detectors, can be detrimental for many imaging applications. Existing algorithms used to numerically remove smear do not contemplate cases where intensity levels change considerably between consecutive frame exposures. In this report, we reformulate the smearing model to include specific variations of the sensor illumination. The corresponding desmearing expression and its noise properties are also presented and demonstrated in the context of fast imaging polarimetry.

Development of a CCD based solar speckle imaging system

NASA Astrophysics Data System (ADS)

Nisenson, Peter; Stachnik, Robert V.; Noyes, Robert W.

1986-02-01

A program to develop software and hardware for the purpose of obtaining high angular resolution images of the solar surface is described. The program included the procurement of a Charge Coupled Devices imaging system; an extensive laboratory and remote site testing of the camera system; the development of a software package for speckle image reconstruction which was eventually installed and tested at the Sacramento Peak Observatory; and experiments of the CCD system (coupled to an image intensifier) for low light level, narrow spectral band solar imaging.

Data Reduction and Control Software for Meteor Observing Stations Based on CCD Video Systems

NASA Technical Reports Server (NTRS)

Madiedo, J. M.; Trigo-Rodriguez, J. M.; Lyytinen, E.

2011-01-01

The SPanish Meteor Network (SPMN) is performing a continuous monitoring of meteor activity over Spain and neighbouring countries. The huge amount of data obtained by the 25 video observing stations that this network is currently operating made it necessary to develop new software packages to accomplish some tasks, such as data reduction and remote operation of autonomous systems based on high-sensitivity CCD video devices. The main characteristics of this software are described here.

Improving signal to noise in labeled biological specimens using energy-filtered TEM of sections with a drift correction strategy and a direct detection device.

PubMed

Ramachandra, Ranjan; Bouwer, James C; Mackey, Mason R; Bushong, Eric; Peltier, Steven T; Xuong, Nguyen-Huu; Ellisman, Mark H

2014-06-01

Energy filtered transmission electron microscopy techniques are regularly used to build elemental maps of spatially distributed nanoparticles in materials and biological specimens. When working with thick biological sections, electron energy loss spectroscopy techniques involving core-loss electrons often require exposures exceeding several minutes to provide sufficient signal to noise. Image quality with these long exposures is often compromised by specimen drift, which results in blurring and reduced resolution. To mitigate drift artifacts, a series of short exposure images can be acquired, aligned, and merged to form a single image. For samples where the target elements have extremely low signal yields, the use of charge coupled device (CCD)-based detectors for this purpose can be problematic. At short acquisition times, the images produced by CCDs can be noisy and may contain fixed pattern artifacts that impact subsequent correlative alignment. Here we report on the use of direct electron detection devices (DDD's) to increase the signal to noise as compared with CCD's. A 3× improvement in signal is reported with a DDD versus a comparably formatted CCD, with equivalent dose on each detector. With the fast rolling-readout design of the DDD, the duty cycle provides a major benefit, as there is no dead time between successive frames.

Protein Crystal Movements and Fluid Flows During Microgravity Growth

NASA Technical Reports Server (NTRS)

Boggon, Titus J.; Chayen, Naomi E.; Snell, Edward H.; Dong, Jun; Lautenschlager, Peter; Potthast, Lothar; Siddons, D. Peter; Stojanoff, Vivian; Gordon, Elspeth; Thompson, Andrew W.;

French Meteor Network for High Precision Orbits of Meteoroids

NASA Technical Reports Server (NTRS)

Atreya, P.; Vaubaillon, J.; Colas, F.; Bouley, S.; Gaillard, B.; Sauli, I.; Kwon, M. K.

2011-01-01

There is a lack of precise meteoroids orbit from video observations as most of the meteor stations use off-the-shelf CCD cameras. Few meteoroids orbit with precise semi-major axis are available using film photographic method. Precise orbits are necessary to compute the dust flux in the Earth s vicinity, and to estimate the ejection time of the meteoroids accurately by comparing them with the theoretical evolution model. We investigate the use of large CCD sensors to observe multi-station meteors and to compute precise orbit of these meteoroids. An ideal spatial and temporal resolution to get an accuracy to those similar of photographic plates are discussed. Various problems faced due to the use of large CCD, such as increasing the spatial and the temporal resolution at the same time and computational problems in finding the meteor position are illustrated.

Excimer laser processing of backside-illuminated CCDS

NASA Technical Reports Server (NTRS)

Russell, S. D.

1993-01-01

An excimer laser is used to activate previously implanted dopants on the backside of a backside-illuminated CCD. The controlled ion implantation of the backside and subsequent thin layer heating and recrystallization by the short wavelength pulsed excimer laser simultaneously activates the dopant and anneals out implant damage. This improves the dark current response, repairs defective pixels and improves spectral response. This process heats a very thin layer of the material to high temperatures on a nanosecond time scale while the bulk of the delicate CCD substrate remains at low temperature. Excimer laser processing backside-illuminated CCD's enables salvage and utilization of otherwise nonfunctional components by bringing their dark current response to within an acceptable range. This process is particularly useful for solid state imaging detectors used in commercial, scientific and government applications requiring a wide spectral response and low light level detection.

A novel imaging method for photonic crystal fiber fusion splicer

NASA Astrophysics Data System (ADS)

Bi, Weihong; Fu, Guangwei; Guo, Xuan

2007-01-01

Because the structure of Photonic Crystal Fiber (PCF) is very complex, and it is very difficult that traditional fiber fusion splice obtains optical axial information of PCF. Therefore, we must search for a bran-new optical imaging method to get section information of Photonic Crystal Fiber. Based on complex trait of PCF, a novel high-precision optics imaging system is presented in this article. The system uses a thinned electron-bombarded CCD (EBCCD) which is a kind of image sensor as imaging element, the thinned electron-bombarded CCD can offer low light level performance superior to conventional image intensifier coupled CCD approaches, this high-performance device can provide high contrast high resolution in low light level surveillance imaging; in order to realize precision focusing of image, we use a ultra-highprecision pace motor to adjust position of imaging lens. In this way, we can obtain legible section information of PCF. We may realize further concrete analysis for section information of PCF by digital image processing technology. Using this section information may distinguish different sorts of PCF, compute some parameters such as the size of PCF ventage, cladding structure of PCF and so on, and provide necessary analysis data for PCF fixation, adjustment, regulation, fusion and cutting system.

Performance characteristics of CCDs for the ACIS experiment. [Advanced X-ray Astrophysics Facility CCD Imaging Spectrometer

NASA Technical Reports Server (NTRS)

Garmire, Gordon P.; Nousek, John; Burrows, David; Ricker, George; Bautz, Mark; Doty, John; Collins, Stewart; Janesick, James

1988-01-01

The search for the optimum CCD to be used at the focal surface of the Advanced X-ray Astrophysics Facility (AXAF) is described. The physics of the interaction of X-rays in silicon through the photoelectric effect is reviewed. CCD technology at the beginning of the AXAF definition phase is summarized, and the results of the CCD enhancement program are discussed. Other sources of optimum CCDs are examined, and CCD enhancements made at MIT Lincoln Laboratory are addressed.

CCD research. [design, fabrication, and applications

NASA Technical Reports Server (NTRS)

Gassaway, J. D.

1976-01-01

The fundamental problems encountered in designing, fabricating, and applying CCD's are reviewed. Investigations are described and results and conclusions are given for the following: (1) the development of design analyses employing computer aided techniques and their application to the design of a grapped structure; (2) the role of CCD's in applications to electronic functions, in particular, signal processing; (3) extending the CCD to silicon films on sapphire (SOS); and (4) all aluminum transfer structure with low noise input-output circuits. Related work on CCD imaging devices is summarized.

Fast Neutron Tomography of Low-Z Object in High-Z Material Shielding

NASA Astrophysics Data System (ADS)

Babai, Ruth Weiss; Sabo-Napadensky, Iris; Bar, Doron; Mor, Ilan; Tamim, Noam; Dangendorf, Volker; Tittelmeier, Kai; Bromberger, Benjamin; Weierganz, Mathias

The technique and first results of Fast Neutron Tomography (FNCT) experiments are presented which are performed at the accelerator facility of PTB, Germany. A high-intensity neutron beam of broad spectral distribution with an average energy of 5.5 MeV, was produced by 11.5 MeV deuterons impinging upon a thick beryllium target. The capability of FNCT for high contrast imaging of low-Z materials embedded in thick high-Z shielding materials is demonstrated, which is superior to more conventional high-energy X-ray imaging techniques. For demonstrating the method special test objects were prepared: One consisted of an assembled polyethylene cylinder with holes of various diameters and directions drilled in its surface and inner parts. The plastic phantom was inserted into lead cylinders of different thicknesses. The detector system consisted of a plastic scintillator along with a dedicated optics, image-intensifier and a CCD camera. Two scintillator screens were compared: a bulk plastic scintillator screen and a fibres optical scintillator screen. The tomographic scans were taken in two geometrical configurations: cone beam and semi-fan beam configuration. The image quality favours the semi-fan beam configuration which on the other hand is more time consuming The obtained tomographic images and a comparison of the imaging quality between the different experimental conditions will be presented.

Design and Development of Multi-Purpose CCD Camera System with Thermoelectric Cooling: Hardware

NASA Astrophysics Data System (ADS)

Kang, Y.-W.; Byun, Y. I.; Rhee, J. H.; Oh, S. H.; Kim, D. K.

2007-12-01

We designed and developed a multi-purpose CCD camera system for three kinds of CCDs; KAF-0401E(768×512), KAF-1602E(1536×1024), KAF-3200E(2184×1472) made by KODAK Co.. The system supports fast USB port as well as parallel port for data I/O and control signal. The packing is based on two stage circuit boards for size reduction and contains built-in filter wheel. Basic hardware components include clock pattern circuit, A/D conversion circuit, CCD data flow control circuit, and CCD temperature control unit. The CCD temperature can be controlled with accuracy of approximately 0.4° C in the max. range of temperature, Δ 33° C. This CCD camera system has with readout noise 6 e^{-}, and system gain 5 e^{-}/ADU. A total of 10 CCD camera systems were produced and our tests show that all of them show passable performance.

Total ionizing dose effect and damage mechanism on saturation output voltage of charge coupled device

NASA Astrophysics Data System (ADS)

Wen, Lin; Li, Yu-dong; Guo, Qi; Wang, Chao-min

2018-02-01

Total ionizing dose effect is a major threat to space applications of CCD, which leads to the decrease of CCD saturation output voltage and the increase of dark signal. This paper investigated CCD and its readout circuit for experimental samples of different channel width to length ratio of MOSFET, and readout circuit amplifier, and CCD. The irradiation source was 60Co- gamma ray. through testing the parameters degradation of MOSFET and amplifier degradation, the generation and annealing law of irradiation induced defects in MOS single tube are analyzed. Combined with the radiation effect of amplifier and CCD, The correlation of radiation damage of the MOSFET and the readout circuit amplifier and CCD parameter degradation is established. Finally, this paper reveals the physical mechanism of ionizing radiation damage of the readout circuit. The research results provide a scientific basis for the selection of anti-radiation technology and structure optimization of domestic CCD.

Developing a more useful surface quality metric for laser optics

NASA Astrophysics Data System (ADS)

Turchette, Quentin; Turner, Trey

2011-02-01

Light scatter due to surface defects on laser resonator optics produces losses which lower system efficiency and output power. The traditional methodology for surface quality inspection involves visual comparison of a component to scratch and dig (SAD) standards under controlled lighting and viewing conditions. Unfortunately, this process is subjective and operator dependent. Also, there is no clear correlation between inspection results and the actual performance impact of the optic in a laser resonator. As a result, laser manufacturers often overspecify surface quality in order to ensure that optics will not degrade laser performance due to scatter. This can drive up component costs and lengthen lead times. Alternatively, an objective test system for measuring optical scatter from defects can be constructed with a microscope, calibrated lighting, a CCD detector and image processing software. This approach is quantitative, highly repeatable and totally operator independent. Furthermore, it is flexible, allowing the user to set threshold levels as to what will or will not constitute a defect. This paper details how this automated, quantitative type of surface quality measurement can be constructed, and shows how its results correlate against conventional loss measurement techniques such as cavity ringdown times.

Advances in detector technologies for visible and infrared wavefront sensing

NASA Astrophysics Data System (ADS)

Feautrier, Philippe; Gach, Jean-Luc; Downing, Mark; Jorden, Paul; Kolb, Johann; Rothman, Johan; Fusco, Thierry; Balard, Philippe; Stadler, Eric; Guillaume, Christian; Boutolleau, David; Destefanis, Gérard; Lhermet, Nicolas; Pacaud, Olivier; Vuillermet, Michel; Kerlain, Alexandre; Hubin, Norbert; Reyes, Javier; Kasper, Markus; Ivert, Olaf; Suske, Wolfgang; Walker, Andrew; Skegg, Michael; Derelle, Sophie; Deschamps, Joel; Robert, Clélia; Vedrenne, Nicolas; Chazalet, Frédéric; Tanchon, Julien; Trollier, Thierry; Ravex, Alain; Zins, Gérard; Kern, Pierre; Moulin, Thibaut; Preis, Olivier

2012-07-01

The purpose of this paper is to give an overview of the state of the art wavefront sensor detectors developments held in Europe for the last decade. The success of the next generation of instruments for 8 to 40-m class telescopes will depend on the ability of Adaptive Optics (AO) systems to provide excellent image quality and stability. This will be achieved by increasing the sampling, wavelength range and correction quality of the wave front error in both spatial and time domains. The modern generation of AO wavefront sensor detectors development started in the late nineties with the CCD50 detector fabricated by e2v technologies under ESO contract for the ESO NACO AO system. With a 128x128 pixels format, this 8 outputs CCD offered a 500 Hz frame rate with a readout noise of 7e-. A major breakthrough has been achieved with the recent development by e2v technologies of the CCD220. This 240x240 pixels 8 outputs EMCCD (CCD with internal multiplication) has been jointly funded by ESO and Europe under the FP6 programme. The CCD220 and the OCAM2 camera that operates the detector are now the most sensitive system in the world for advanced adaptive optics systems, offering less than 0.2 e readout noise at a frame rate of 1500 Hz with negligible dark current. Extremely easy to operate, OCAM2 only needs a 24 V power supply and a modest water cooling circuit. This system, commercialized by First Light Imaging, is extensively described in this paper. An upgrade of OCAM2 is foreseen to boost its frame rate to 2 kHz, opening the window of XAO wavefront sensing for the ELT using 4 synchronized cameras and pyramid wavefront sensing. Since this major success, new developments started in Europe. One is fully dedicated to Natural and Laser Guide Star AO for the E-ELT with ESO involvement. The spot elongation from a LGS Shack Hartman wavefront sensor necessitates an increase of the pixel format. Two detectors are currently developed by e2v. The NGSD will be a 880x840 pixels CMOS detector with a readout noise of 3 e (goal 1e) at 700 Hz frame rate. The LGSD is a scaling of the NGSD with 1760x1680 pixels and 3 e readout noise (goal 1e) at 700 Hz (goal 1000 Hz) frame rate. New technologies will be developed for that purpose: advanced CMOS pixel architecture, CMOS back thinned and back illuminated device for very high QE, full digital outputs with signal digital conversion on chip. In addition, the CMOS technology is extremely robust in a telescope environment. Both detectors will be used on the European ELT but also interest potentially all giant telescopes under development. Additional developments also started for wavefront sensing in the infrared based on a new technological breakthrough using ultra low noise Avalanche Photodiode (APD) arrays within the RAPID project. Developed by the SOFRADIR and CEA/LETI manufacturers, the latter will offer a 320x240 8 outputs 30 microns IR array, sensitive from 0.4 to 3.2 microns, with 2 e readout noise at 1500 Hz frame rate. The high QE response is almost flat over this wavelength range. Advanced packaging with miniature cryostat using liquid nitrogen free pulse tube cryocoolers is currently developed for this programme in order to allow use on this detector in any type of environment. First results of this project are detailed here. These programs are held with several partners, among them are the French astronomical laboratories (LAM, OHP, IPAG), the detector manufacturers (e2v technologies, Sofradir, CEA/LETI) and other partners (ESO, ONERA, IAC, GTC). Funding is: Opticon FP6 and FP7 from European Commission, ESO, CNRS and Université de Provence, Sofradir, ONERA, CEA/LETI and the French FUI (DGCIS).

Next generation of pnCCDs for X-ray spectroscopy and imaging

NASA Astrophysics Data System (ADS)

Meidinger, Norbert; Andritschke, Robert; Hälker, Olaf; Hartmann, Robert; Herrmann, Sven; Holl, Peter; Lutz, Gerhard; Kimmel, Nils; Schaller, Gerhard; Schnecke, Martina; Schopper, Florian; Soltau, Heike; Strüder, Lothar

2006-11-01

A special type of charge-coupled device, the pnCCD, has been developed in the nineties as focal-plane detector for the X-ray astronomy mission XMM-Newton of the European Space Agency. The pnCCD detector has been in operation since the satellite launch in 1999. It is performing up to date spectroscopy of X-rays in combination with imaging and high time resolution. The excellent performance of the flight camera is still maintained; in particular, the energy resolution has been nearly constant since launch. In order to satisfy the requirements of future X-ray astronomy missions as well as those of ground-based experiments, a new type of pnCCD has been developed. The ‘frame store pnCCD’ shows various optimizations in device design and fabrication process. Devices with up to 256×512 pixels have been fabricated in 2004 and recently tested. Simultaneously, a programmable analog signal processor for the readout of the CCD signals, the DUO CAMEX, has been developed. The readout noise of the new detector has a value of 2 electrons ENC which is less than half of the figure of the XMM-Newton pnCCD. We measured an energy resolution that is close to the theoretical limit given by the Fano noise. In particular the low-energy response of the new devices was substantially improved. The quantum efficiency for X-rays is at least 90% in the entire energy band from 0.3 keV up to 11 keV. This is due to the ultra-thin photon entrance window as well as the full depletion of the 450 μm thick back-illuminated pnCCD. The position resolution is better than the pixel sizes of 75 μm×75 μm or 51 μm×51 μm because the signal charge is spread over up to four pixels which allows a more accurate event position determination. ‘Out of time’ events are substantially reduced to the order of 0.1% by operating the pnCCD in frame store mode. Higher operating temperatures, e.g. -20 °C, are possible due to the smaller thermally generated dark-current level of the new devices and the operation at higher frame rates. Low power consumption applications like for the ROSITA X-ray astronomy mission with low frame rates of, e.g. 20 images/s, as well as high frame rate applications, e.g. 200 images/s, are possible with the same device.

Hyper Suprime-Cam: Camera dewar design

NASA Astrophysics Data System (ADS)

Komiyama, Yutaka; Obuchi, Yoshiyuki; Nakaya, Hidehiko; Kamata, Yukiko; Kawanomoto, Satoshi; Utsumi, Yousuke; Miyazaki, Satoshi; Uraguchi, Fumihiro; Furusawa, Hisanori; Morokuma, Tomoki; Uchida, Tomohisa; Miyatake, Hironao; Mineo, Sogo; Fujimori, Hiroki; Aihara, Hiroaki; Karoji, Hiroshi; Gunn, James E.; Wang, Shiang-Yu

2018-01-01

This paper describes the detailed design of the CCD dewar and the camera system which is a part of the wide-field imager Hyper Suprime-Cam (HSC) on the 8.2 m Subaru Telescope. On the 1.°5 diameter focal plane (497 mm in physical size), 116 four-side buttable 2 k × 4 k fully depleted CCDs are tiled with 0.3 mm gaps between adjacent chips, which are cooled down to -100°C by two pulse tube coolers with a capability to exhaust 100 W heat at -100°C. The design of the dewar is basically a natural extension of Suprime-Cam, incorporating some improvements such as (1) a detailed CCD positioning strategy to avoid any collision between CCDs while maximizing the filling factor of the focal plane, (2) a spherical washers mechanism adopted for the interface points to avoid any deformation caused by the tilt of the interface surface to be transferred to the focal plane, (3) the employment of a truncated-cone-shaped window, made of synthetic silica, to save the back focal space, and (4) a passive heat transfer mechanism to exhaust efficiently the heat generated from the CCD readout electronics which are accommodated inside the dewar. Extensive simulations using a finite-element analysis (FEA) method are carried out to verify that the design of the dewar is sufficient to satisfy the assigned errors. We also perform verification tests using the actually assembled CCD dewar to supplement the FEA and demonstrate that the design is adequate to ensure an excellent image quality which is key to the HSC. The details of the camera system, including the control computer system, are described as well as the assembling process of the dewar and the process of installation on the telescope.

CCD high-speed videography system with new concepts and techniques

NASA Astrophysics Data System (ADS)

Zheng, Zengrong; Zhao, Wenyi; Wu, Zhiqiang

1997-05-01

A novel CCD high speed videography system with brand-new concepts and techniques is developed by Zhejiang University recently. The system can send a series of short flash pulses to the moving object. All of the parameters, such as flash numbers, flash durations, flash intervals, flash intensities and flash colors, can be controlled according to needs by the computer. A series of moving object images frozen by flash pulses, carried information of moving object, are recorded by a CCD video camera, and result images are sent to a computer to be frozen, recognized and processed with special hardware and software. Obtained parameters can be displayed, output as remote controlling signals or written into CD. The highest videography frequency is 30,000 images per second. The shortest image freezing time is several microseconds. The system has been applied to wide fields of energy, chemistry, medicine, biological engineering, aero- dynamics, explosion, multi-phase flow, mechanics, vibration, athletic training, weapon development and national defense engineering. It can also be used in production streamline to carry out the online, real-time monitoring and controlling.

Design and realization of adaptive optical principle system without wavefront sensing

NASA Astrophysics Data System (ADS)

Wang, Xiaobin; Niu, Chaojun; Guo, Yaxing; Han, Xiang'e.

2018-02-01

In this paper, we focus on the performance improvement of the free space optical communication system and carry out the research on wavefront-sensorless adaptive optics. We use a phase only liquid crystal spatial light modulator (SLM) as the wavefront corrector. The optical intensity distribution of the distorted wavefront is detected by a CCD. We develop a wavefront controller based on ARM and a software based on the Linux operating system. The wavefront controller can control the CCD camera and the wavefront corrector. There being two SLMs in the experimental system, one simulates atmospheric turbulence and the other is used to compensate the wavefront distortion. The experimental results show that the performance quality metric (the total gray value of 25 pixels) increases from 3037 to 4863 after 200 iterations. Besides, it is demonstrated that our wavefront-sensorless adaptive optics system based on SPGD algorithm has a good performance in compensating wavefront distortion.

Fatty acids bind tightly to the N-terminal domain of angiopoietin-like protein 4 and modulate its interaction with lipoprotein lipase.

PubMed

Robal, Terje; Larsson, Mikael; Martin, Miina; Olivecrona, Gunilla; Lookene, Aivar

2012-08-24

Angiopoietin-like protein 4 (Angptl4), a potent regulator of plasma triglyceride metabolism, binds to lipoprotein lipase (LPL) through its N-terminal coiled-coil domain (ccd-Angptl4) inducing dissociation of the dimeric enzyme to inactive monomers. In this study, we demonstrate that fatty acids reduce the inactivation of LPL by Angptl4. This was the case both with ccd-Angptl4 and full-length Angptl4, and the effect was seen in human plasma or in the presence of albumin. The effect decreased in the sequence oleic acid > palmitic acid > myristic acid > linoleic acid > linolenic acid. Surface plasmon resonance, isothermal titration calorimetry, fluorescence, and chromatography measurements revealed that fatty acids bind with high affinity to ccd-Angptl4. The interactions were characterized by fast association and slow dissociation rates, indicating formation of stable complexes. The highest affinity for ccd-Angptl4 was detected for oleic acid with a subnanomolar equilibrium dissociation constant (K(d)). The K(d) values for palmitic and myristic acid were in the nanomolar range. Linoleic and linolenic acid bound with much lower affinity. On binding of fatty acids, ccd-Angptl4 underwent conformational changes resulting in a decreased helical content, weakened structural stability, dissociation of oligomers, and altered fluorescence properties of the Trp-38 residue that is located close to the putative LPL-binding region. Based on these results, we propose that fatty acids play an important role in modulating the effects of Angptl4.

Atmospheric correction of HJ-1 CCD imagery over turbid lake waters.

PubMed

Zhang, Minwei; Tang, Junwu; Dong, Qing; Duan, Hongtao; Shen, Qian

2014-04-07

We have presented an atmospheric correction algorithm for HJ-1 CCD imagery over Lakes Taihu and Chaohu with highly turbid waters. The Rayleigh scattering radiance (Lr) is calculated using the hyperspectral Lr with a wavelength interval 1nm. The hyperspectral Lr is interpolated from Lr in the central wavelengths of MODIS bands, which are converted from the band response-averaged Lr calculated using the Rayleigh look up tables (LUTs) in SeaDAS6.1. The scattering radiance due to aerosol (La) is interpolated from La at MODIS band 869nm, which is derived from MODIS imagery using a shortwave infrared atmospheric correction scheme. The accuracy of the atmospheric correction algorithm is firstly evaluated by comparing the CCD measured remote sensing reflectance (Rrs) with MODIS measurements, which are validated by the in situ data. The CCD measured Rrs is further validated by the in situ data for a total of 30 observation stations within ± 1h time window of satellite overpass and field measurements. The validation shows the mean relative errors about 0.341, 0.259, 0.293 and 0.803 at blue, green, red and near infrared bands.

Precise CCD positions of Himalia using Gaia DR1 in 2015-2016

NASA Astrophysics Data System (ADS)

Peng, H. W.; Peng, Q. Y.; Wang, N.

2017-05-01

In order to obtain high-precision CCD positions of Himalia, the sixth Jovian satellite, a total of 598 CCD observations have been obtained during the years 2015-2016. The observations were made by using the 2.4 and 1 m telescopes administered by Yunnan Observatories over 27 nights. Several factors that would influence the positional precision of Himalia were analysed, including the reference star catalogue used, the geometric distortion and the phase effect. By taking advantage of its unprecedented positional precision, the recently released catalogue Gaia Data Release 1 was chosen to match reference stars in the CCD frames of both Himalia and open clusters, which were observed for deriving the geometric distortion. The latest version of sofa library was used to calculate the positions of reference stars. The theoretical positions of Himalia were retrieved from the Jet Propulsion Laboratory Horizons System that includes the satellite ephemeris JUP300, while the positions of Jupiter were based on the planetary ephemeris DE431. Our results showed that the means of observed minus computed (O - C) residuals are 0.071 and -0.001 arcsec in right ascension and declination, respectively. Their standard deviations are estimated at about 0.03 arcsec in each direction.

VUV testing of science cameras at MSFC: QE measurement of the CLASP flight cameras

NASA Astrophysics Data System (ADS)

Champey, P.; Kobayashi, K.; Winebarger, A.; Cirtain, J.; Hyde, D.; Robertson, B.; Beabout, B.; Beabout, D.; Stewart, M.

2015-08-01

The NASA Marshall Space Flight Center (MSFC) has developed a science camera suitable for sub-orbital missions for observations in the UV, EUV and soft X-ray. Six cameras were built and tested for the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP), a joint MSFC, National Astronomical Observatory of Japan (NAOJ), Instituto de Astrofisica de Canarias (IAC) and Institut D'Astrophysique Spatiale (IAS) sounding rocket mission. The CLASP camera design includes a frame-transfer e2v CCD57-10 512 × 512 detector, dual channel analog readout and an internally mounted cold block. At the flight CCD temperature of -20C, the CLASP cameras exceeded the low-noise performance requirements (<= 25 e- read noise and <= 10 e- /sec/pixel dark current), in addition to maintaining a stable gain of ≍ 2.0 e-/DN. The e2v CCD57-10 detectors were coated with Lumogen-E to improve quantum efficiency (QE) at the Lyman- wavelength. A vacuum ultra-violet (VUV) monochromator and a NIST calibrated photodiode were employed to measure the QE of each camera. Three flight cameras and one engineering camera were tested in a high-vacuum chamber, which was configured to operate several tests intended to verify the QE, gain, read noise and dark current of the CCD. We present and discuss the QE measurements performed on the CLASP cameras. We also discuss the high-vacuum system outfitted for testing of UV, EUV and X-ray science cameras at MSFC.

Trap pumping schemes for the Euclid CCD273 detector: characterisation of electrodes and defects

NASA Astrophysics Data System (ADS)

Skottfelt, J.; Hall, D. J.; Dryer, B.; Bush, N.; Campa, J.; Gow, J. P. D.; Holland, A. D.; Jordan, D.; Burt, D.

2017-12-01

The VISible imager instrument (VIS) on board the Euclid mission will deliver high resolution shape measurements of galaxies down to very faint limits (R ~ 25 at 10σ) in a large part of the sky, in order to infer the distribution of dark matter in the Universe. To help mitigate radiation damage effects that will accumulate in the detectors over the mission lifetime, the properties of the radiation induced traps needs to be known with as high precision as possible. For this purpose the trap pumping method will be employed as part of the in-orbit calibration routines. Using trap pumping it is possible to identify and characterise single traps in a Charge-Coupled Device (CCD), thus providing information such as the density, emission time constants and sub-pixel positions of the traps in the detectors. This paper presents the trap pumping algorithms used for the radiation testing campaign of the CCD273 detectors, performed by the Centre for Electronic Imaging (CEI) at the Open University, that will be used for the VIS instrument. The CCD273 is a four-phase device with uneven phase widths, which complicates the trap pumping analysis. However, we find that by optimising the trap pumping algorithms and analysis routines, it is possible to obtain sub-pixel and even sub-phase positional information about the traps. Further, by comparing trap pumping data with simulations, it is possible to gain more information about the effective electrode widths of the device.

Cameras for digital microscopy.

PubMed

Spring, Kenneth R

2013-01-01

This chapter reviews the fundamental characteristics of charge-coupled devices (CCDs) and related detectors, outlines the relevant parameters for their use in microscopy, and considers promising recent developments in the technology of detectors. Electronic imaging with a CCD involves three stages--interaction of a photon with the photosensitive surface, storage of the liberated charge, and readout or measurement of the stored charge. The most demanding applications in fluorescence microscopy may require as much as four orders of greater magnitude sensitivity. The image in the present-day light microscope is usually acquired with a CCD camera. The CCD is composed of a large matrix of photosensitive elements (often referred to as "pixels" shorthand for picture elements, which simultaneously capture an image over the entire detector surface. The light-intensity information for each pixel is stored as electronic charge and is converted to an analog voltage by a readout amplifier. This analog voltage is subsequently converted to a numerical value by a digitizer situated on the CCD chip, or very close to it. Several (three to six) amplifiers are required for each pixel, and to date, uniform images with a homogeneous background have been a problem because of the inherent difficulties of balancing the gain in all of the amplifiers. Complementary metal oxide semiconductor sensors also exhibit relatively high noise associated with the requisite high-speed switching. Both of these deficiencies are being addressed, and sensor performance is nearing that required for scientific imaging. Copyright © 1998 Elsevier Inc. All rights reserved.

Reaching the Diffraction Limit - Differential Speckle and Wide-Field Imaging for the Gemini-N Telescope

NASA Technical Reports Server (NTRS)

Scott, Nic J.; Howell, Steve; Horch, Elliott

2016-01-01

Speckle imaging allows telescopes to achieve di raction limited imaging performance. The technique requires cameras capable of reading out frames at a very fast rate, e ectively `freezing out' atmospheric seeing. The resulting speckles can be correlated and images reconstructed that are at the di raction limit of the telescope. These new instruments are based on the successful performance and design of the Di erential Speckle Survey Instrument (DSSI) [2, 1]. The instruments are being built for the Gemini-N and WIYN telescopes and will be made available to the community via the peer review proposal process. We envision their primary use to be validation and characterization of exoplanet targets from the NASA K2 and TESS missions and RV discovered exoplanets. Such targets will provide excellent follow-up candidates for both the WIYN and Gemini telescopes [3]. Examples of DSSI data are shown in the gures below. We expect similar data quality in speckle imaging mode with the new instruments. Additionally, both cameras will have a wide- eld mode and standard SDSS lters. They will be highly versatile instruments and it is that likely many other science programs will request time on the cameras. The limiting magnitude for speckle observations, will remain around 13-14th at WIYN and 16-17th at Gemini, while wide- eld, normal CCD imaging operation should be able to go to much fainter, providing usual CCD imaging and photometric capabilities. The instruments will also have high utility as scoring cameras for telescope engineering purposes, or other applications where high time resolution is needed. Instrument support will be provided, including a software pipeline that takes raw speckle data to fully reconstructed images.

A CMOS In-Pixel CTIA High Sensitivity Fluorescence Imager.

PubMed

Murari, Kartikeya; Etienne-Cummings, Ralph; Thakor, Nitish; Cauwenberghs, Gert

2011-10-01

Traditionally, charge coupled device (CCD) based image sensors have held sway over the field of biomedical imaging. Complementary metal oxide semiconductor (CMOS) based imagers so far lack sensitivity leading to poor low-light imaging. Certain applications including our work on animal-mountable systems for imaging in awake and unrestrained rodents require the high sensitivity and image quality of CCDs and the low power consumption, flexibility and compactness of CMOS imagers. We present a 132×124 high sensitivity imager array with a 20.1 μm pixel pitch fabricated in a standard 0.5 μ CMOS process. The chip incorporates n-well/p-sub photodiodes, capacitive transimpedance amplifier (CTIA) based in-pixel amplification, pixel scanners and delta differencing circuits. The 5-transistor all-nMOS pixel interfaces with peripheral pMOS transistors for column-parallel CTIA. At 70 fps, the array has a minimum detectable signal of 4 nW/cm(2) at a wavelength of 450 nm while consuming 718 μA from a 3.3 V supply. Peak signal to noise ratio (SNR) was 44 dB at an incident intensity of 1 μW/cm(2). Implementing 4×4 binning allowed the frame rate to be increased to 675 fps. Alternately, sensitivity could be increased to detect about 0.8 nW/cm(2) while maintaining 70 fps. The chip was used to image single cell fluorescence at 28 fps with an average SNR of 32 dB. For comparison, a cooled CCD camera imaged the same cell at 20 fps with an average SNR of 33.2 dB under the same illumination while consuming over a watt.

A CMOS In-Pixel CTIA High Sensitivity Fluorescence Imager

PubMed Central

Murari, Kartikeya; Etienne-Cummings, Ralph; Thakor, Nitish; Cauwenberghs, Gert

2012-01-01

Traditionally, charge coupled device (CCD) based image sensors have held sway over the field of biomedical imaging. Complementary metal oxide semiconductor (CMOS) based imagers so far lack sensitivity leading to poor low-light imaging. Certain applications including our work on animal-mountable systems for imaging in awake and unrestrained rodents require the high sensitivity and image quality of CCDs and the low power consumption, flexibility and compactness of CMOS imagers. We present a 132×124 high sensitivity imager array with a 20.1 μm pixel pitch fabricated in a standard 0.5 μ CMOS process. The chip incorporates n-well/p-sub photodiodes, capacitive transimpedance amplifier (CTIA) based in-pixel amplification, pixel scanners and delta differencing circuits. The 5-transistor all-nMOS pixel interfaces with peripheral pMOS transistors for column-parallel CTIA. At 70 fps, the array has a minimum detectable signal of 4 nW/cm2 at a wavelength of 450 nm while consuming 718 μA from a 3.3 V supply. Peak signal to noise ratio (SNR) was 44 dB at an incident intensity of 1 μW/cm2. Implementing 4×4 binning allowed the frame rate to be increased to 675 fps. Alternately, sensitivity could be increased to detect about 0.8 nW/cm2 while maintaining 70 fps. The chip was used to image single cell fluorescence at 28 fps with an average SNR of 32 dB. For comparison, a cooled CCD camera imaged the same cell at 20 fps with an average SNR of 33.2 dB under the same illumination while consuming over a watt. PMID:23136624

Influence of camera parameters on the quality of mobile 3D capture

NASA Astrophysics Data System (ADS)

Georgiev, Mihail; Boev, Atanas; Gotchev, Atanas; Hannuksela, Miska

2010-01-01

We investigate the effect of camera de-calibration on the quality of depth estimation. Dense depth map is a format particularly suitable for mobile 3D capture (scalable and screen independent). However, in real-world scenario cameras might move (vibrations, temp. bend) form their designated positions. For experiments, we create a test framework, described in the paper. We investigate how mechanical changes will affect different (4) stereo-matching algorithms. We also assess how different geometric corrections (none, motion compensation-like, full rectification) will affect the estimation quality (how much offset can be still compensated with "crop" over a larger CCD). Finally, we show how estimated camera pose change (E) relates with stereo-matching, which can be used for "rectification quality" measure.

SU-F-T-180: Evaluation of a Scintillating Screen Detector for Proton Beam QA and Acceptance Testing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ghebremedhin, A; Taber, M; Koss, P

2016-06-15

Purpose: To test the performance of a commercial scintillating screen detector for acceptance testing and Quality Assurance of a proton pencil beam scanning system. Method: The detector (Lexitek DRD 400) has 40cm × 40cm field, uses a thin scintillator imaged onto a 16-bit scientific CCD with ∼0.5mm resolution. A grid target and LED illuminators are provided for spatial calibration and relative gain correction. The detector mounts to the nozzle with micron precision. Tools are provided for image processing and analysis of single or multiple Gaussian spots. Results: The bias and gain of the detector were studied to measure repeatability andmore » accuracy. Gain measurements were taken with the LED illuminators to measure repeatability and variation of the lens-CCD pair as a function with f-stop. Overall system gain was measured with a passive scattering (broad) beam whose shape is calibrated with EDR film placed in front of the scintillator. To create a large uniform field, overlapping small fields were recorded with the detector translated laterally and stitched together to cover the full field. Due to the long exposures required to obtain multiple spills of the synchrotron and very high detector sensitivity, borated polyethylene shielding was added to reduce direct radiation events hitting the CCD. Measurements with a micro ion chamber were compared to the detector’s spot profile. Software was developed to process arrays of Gaussian spots and to correct for radiation events. Conclusion: The detector background has a fixed bias, a small component linear in time, and is easily corrected. The gain correction method was validated with 2% accuracy. The detector spot profile matches the micro ion chamber data over 4 orders of magnitude. The multiple spot analyses can be easily used with plan data for measuring pencil beam uniformity and for regular QA comparison.« less

X-Ray Spectroscopy of Optically Bright Planets using the Chandra Observatory

NASA Technical Reports Server (NTRS)

Ford, P. G.; Elsner, R. F.

2005-01-01

Since its launch in July 1999, Chandra's Advanced CCD Imaging Spectrometer (ACIS) has observed several planets (Venus, Mars, Jupiter and Saturn) and 6 comets. At 0.5 arc-second spatial resolution, ACIS detects individual x-ray photons with good quantum efficiency (25% at 0.6 KeV) and energy resolution (20% FWHM at 0.6 KeV). However, the ACIS CCDs are also sensitive to optical and near-infrared light, which is absorbed by optical blocking filters (OBFs) that eliminate optical contamination from all but the brightest extended sources, e.g., planets. .Jupiter at opposition subseconds approx.45 arc-seconds (90 CCD pixels.) Since Chandra is incapable of tracking a moving target, the planet takes 10 - 20 kiloseconds to move across the most sensitive ACIS CCD, after which the observatory must be re-pointed. Meanwhile, the OBF covering that CCD adds an opt,ical signal equivalent to approx.110 eV to each pixel that lies within thc outline of the Jovian disk. This has three consequences: (1) the observatory must be pointed away from Jupiter while CCD bias maps are constructed; (2) most x-rays from within the optical image will be misidentified as charged-particle background and ignored; and (3) those x-rays that are reported will bc assigned anomalously high energies. The same also applies to thc other planets, but is less serious since they are either dimmer at optical wavelengths, or they show less apparent motion across the sky, permitting reduced CCD exposure times: the optical contamination from Saturn acids approx.15 eV per pixel, and from Mars and Venus approx.31 eV. After analyzing a series of short .Jupiter observations in December 2000, ACIS parameters were optimized for the February 2003 opposition. CCD bias maps were constructed while Chandra pointed away from Jupiter, and the subsequent observations employed on-board software to ignore any pixel that contained less charge than that expected from optical leakage. In addition, ACIS was commanded to report 5 x 5 arrays of pixel values surrounding each x-ray event, and the outlying values were employed during ground processing to correct for the optical contamination.

Genome sequencing reveals a splice donor site mutation in the SNX14 gene associated with a novel cerebellar cortical degeneration in the Hungarian Vizsla dog breed.

PubMed

Fenn, Joe; Boursnell, Mike; Hitti, Rebekkah J; Jenkins, Christopher A; Terry, Rebecca L; Priestnall, Simon L; Kenny, Patrick J; Mellersh, Cathryn S; Forman, Oliver P

2016-08-26

Cerebellar cortical degeneration (CCD) is an increasingly recognised neurodegenerative disease process affecting many dog breeds. Typical presentation consists of a progressive cerebellar ataxia, with a variable age at onset and rate of progression between different breeds. Cerebellar histopathological findings typically consist of primary Purkinje neuronal degeneration and loss, with variable secondary depletion of the granular and molecular cell layers. Causative genes have been identified associated with CCD in several breeds, allowing screening for selective breeding to reduce the prevalence of these conditions. There have been no previous reports of CCD in Hungarian Vizslas. Two full-sibling Hungarian Vizsla puppies from a litter of nine presented with a history of progressive ataxia, starting around three months of age. Clinical signs included marked hypermetric and dysmetric ataxia, truncal sway, intention tremors and absent menace responses, with positional horizontal nystagmus in one dog. Routine diagnostic investigations were unremarkable, and magnetic resonance imaging performed in one dog revealed mild craniodorsal cerebellar sulci widening, supportive of cerebellar atrophy. Owners of both dogs elected for euthanasia shortly after the onset of signs. Histopathological examination revealed primary Purkinje neuron loss consistent with CCD. Whole genome sequencing was used to successfully identify a disease-associated splice donor site variant in the sorting nexin 14 gene (SNX14) as a strong causative candidate. An altered SNX14 splicing pattern for a CCD case was demonstrated by RNA analysis, and no SNX14 protein could be detected in CCD case cerebellum by western blotting. SNX14 is involved in maintaining normal neuronal excitability and synaptic transmission, and a mutation has recently been found to cause autosomal recessive cerebellar ataxia and intellectual disability syndrome in humans. Genetic screening of 133 unaffected Hungarian Vizslas revealed the presence of three heterozygotes, supporting the presence of carriers in the wider population. This is the first report of CCD in Hungarian Vizsla dogs and identifies a highly associated splice donor site mutation in SNX14, with an autosomal recessive mode of inheritance suspected.

Remote sensing of potential lunar resources. I - Near-side compositional properties

NASA Technical Reports Server (NTRS)

Johnson, Jeffrey R.; Larson, Stephen M.; Singer, Robert B.

1991-01-01

Using telescopic CCD multispectral images of the lunar near side and the results of 330-870 nm spectroscopy of selected regions, the compositional differences relevant to the locations of potential lunar resources (such as ilmenite, FeTiO3, and solar-wind-implanted He-3 and H) are estimated. The 400/560 nm CCD ratio images were converted to weight percent TiO2, and the values were used to construct a new TiO2 abundance map which can be used to estimate the areas potentially rich in ilmenite. A 950/560 nm CCD ratio mosaic of the full moon provides estimates of relative surface maturity. Since high He-3 concentrations correlate with mature ilmenite-rich soils, a combination of relative surface maturity maps and the TiO2 abundance maps can be used to estimate distributions of He-3 (and possibly H) on local scales.

Growth of delta-doped layers on silicon CCD/S for enhanced ultraviolet response

NASA Technical Reports Server (NTRS)

Hoenk, Michael E. (Inventor); Grunthaner, Paula J. (Inventor); Grunthaner, Frank J. (Inventor); Terhune, Robert W. (Inventor); Hecht, Michael H. (Inventor)

1994-01-01

The backside surface potential well of a backside-illuminated CCD is confined to within about half a nanometer of the surface by using molecular beam epitaxy (MBE) to grow a delta-doped silicon layer on the back surface. Delta-doping in an MBE process is achieved by temporarily interrupting the evaporated silicon source during MBE growth without interrupting the evaporated p+ dopant source (e.g., boron). This produces an extremely sharp dopant profile in which the dopant is confined to only a few atomic layers, creating an electric field high enough to confine the backside surface potential well to within half a nanometer of the surface. Because the probability of UV-generated electrons being trapped by such a narrow potential well is low, the internal quantum efficiency of the CCD is nearly 100% throughout the UV wavelength range. Furthermore, the quantum efficiency is quite stable.

X-ray polarimeter with a transmission multilayer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kitamoto, Shunji; Murakami, Hiroshi; Shishido, Youich

2010-02-15

We fabricated a novel x-ray polarimeter with a transmission multilayer and measured its performance with synchrotron radiation. A self standing multilayer with seven Mo/Si bilayers was installed with an incident angle of 45 deg. in front of a back-illuminated CCD. The multilayer can be rotated around the normal direction of the CCD keeping an incident angle of 45 deg. This polarimeter can be easily installed along the optical axis of x-ray optics. By using the CCD as a photon counting detector with a moderate energy resolution, the polarization of photons in a designed energy band can be measured along withmore » the image. At high photon energies, where the multilayer is transparent, the polarimeter can be used for imaging and spectroscopic observations. We confirmed a modulation factor of 45% with 45% and 17% transmission for P- and S-polarization, respectively.« less

Kottamia 74-inch telescope discovery of the new eclipsing binary 2MASS J20004638 + 0547475.: First CCD photometry and light curve analysis

NASA Astrophysics Data System (ADS)

Darwish, M. S.; Shokry, A.; Saad, S. M.; El-Sadek, M. A.; Essam, A.; Ismail, M.

2017-05-01

A CCD photometric study is presented for the eclipsing binary system 2MASS J20004638 + 0547475. Observations of the system were obtained in the V, R and I colours with the 2Kx2K CCD attached to 1.88 m Kottamia Optical Telescope. New times of light minimum and new ephemeris were obtained. The V, R and I light curves were analyzed using the PHOEBE 0.31 program to determine geometrical and physical parameters of the system. The results show that 2MASS J20004638 + 0547475, is A-Type WUMa and is an overcontact binary with high fill-out factor = 69%. The current evolutionary status of the system indicates that the primary component lies very close to the main sequence while the secondary is evolved. The asymmetric maxima were studied and a modeling of the hot spot parameters is given.

New hydrolytically stable solvent for Am/Eu separation in acidic media

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smirnov, I.V.; Babain, V.A.; Chirkov, A.V.

Americium and europium extraction by synergistic mixture of 2,6-bis(1-aryl-1H-tetrazol-5-yl)pyridines (ATP) - chlorinated cobalt dicarbollide (CCD) in polar diluent s from HNO{sub 3} media was studied. Meta-nitro-benzo-trifluoride, phenyl-tri-fluoro-methyl sulfone and 1,2-dichloroethane were used as diluents. The effect of diluent, composition of aqueous phase and substituent nature in aryl ring of ATPs on the extraction efficiency and selectivity of americium and europium separation was investigated. At the optimal ratio of nATP:CCD 1:1 the Am - Eu separation factor exceeded 90. Extraction of {sup 85}Sr, {sup 137}Cs and {sup 133}Ba was investigated and it was found that the mixture nATP-CCD provided the separationmore » of Sr /Ba pair with a factor of 35. High resistance of 2,6-bisaryltetrazolyl pyridines to the action of nitric acid was demonstrated. (authors)« less

Fast noninvasive eye-tracking and eye-gaze determination for biomedical and remote monitoring applications

NASA Astrophysics Data System (ADS)

Talukder, Ashit; Morookian, John M.; Monacos, Steve P.; Lam, Raymond K.; Lebaw, C.; Bond, A.

2004-04-01

Eyetracking is one of the latest technologies that has shown potential in several areas including human-computer interaction for people with and without disabilities, and for noninvasive monitoring, detection, and even diagnosis of physiological and neurological problems in individuals. Current non-invasive eyetracking methods achieve a 30 Hz rate with possibly low accuracy in gaze estimation, that is insufficient for many applications. We propose a new non-invasive visual eyetracking system that is capable of operating at speeds as high as 6-12 KHz. A new CCD video camera and hardware architecture is used, and a novel fast image processing algorithm leverages specific features of the input CCD camera to yield a real-time eyetracking system. A field programmable gate array (FPGA) is used to control the CCD camera and execute the image processing operations. Initial results show the excellent performance of our system under severe head motion and low contrast conditions.

Characterization of OfWRKY3, a transcription factor that positively regulates the carotenoid cleavage dioxygenase gene OfCCD4 in Osmanthus fragrans.

PubMed

Han, Yuanji; Wu, Miao; Cao, Liya; Yuan, Wangjun; Dong, Meifang; Wang, Xiaohui; Chen, Weicai; Shang, Fude

2016-07-01

The sweet osmanthus carotenoid cleavage dioxygenase 4 (OfCCD4) cleaves carotenoids such as β-carotene and zeaxanthin to yield β-ionone. OfCCD4 is a member of the CCD gene family, and its promoter contains a W-box palindrome with two reversely oriented TGAC repeats, which are the proposed binding sites of WRKY transcription factors. We isolated three WRKY cDNAs from the petal of Osmanthus fragrans. One of them, OfWRKY3, encodes a protein containing two WRKY domains and two zinc finger motifs. OfWRKY3 and OfCCD4 had nearly identical expression profile in petals of 'Dangui' and 'Yingui' at different flowering stages and showed similar expression patterns in petals treated by salicylic acid, jasmonic acid and abscisic acid. Activation of OfCCD4pro:GUS by OfWRKY3 was detected in coinfiltrated tobacco leaves and very weak GUS activity was detected in control tissues, indicating that OfWRKY3 can interact with the OfCCD4 promoter. Yeast one-hybrid and electrophoretic mobility shift assay showed that OfWRKY3 was able to bind to the W-box palindrome motif present in the OfCCD4 promoter. These results suggest that OfWRKY3 is a positive regulator of the OfCCD4 gene, and might partly account for the biosynthesis of β-ionone in sweet osmanthus.

Spectral analysis using the CCD Chirp Z-transform

NASA Technical Reports Server (NTRS)

Eversole, W. L.; Mayer, D. J.; Bosshart, P. W.; Dewit, M.; Howes, C. R.; Buss, D. D.

1978-01-01

The charge coupled device (CCD) Chirp Z transformation (CZT) spectral analysis techniques were reviewed and results on state-of-the-art CCD CZT technology are presented. The CZT algorithm was examined and the advantages of CCD implementation are discussed. The sliding CZT which is useful in many spectral analysis applications is described, and the performance limitations of the CZT are studied.

Documentation to the 2015-16 Common Core of Data (CCD) Universe Files. NCES 2017-074

ERIC Educational Resources Information Center

Glander, Mark

2017-01-01

The Common Core of Data (CCD) is a national statistical program that collects and compiles administrative data from SEAs covering the universe of all public elementary and secondary schools and school districts in the United States. The first CCD collection was for SY 1986-87. The predecessor to CCD was the Elementary and Secondary General…

Proton radiation damage experiment on P-Channel CCD for an X-ray CCD camera onboard the ASTRO-H satellite

NASA Astrophysics Data System (ADS)

Mori, Koji; Nishioka, Yusuke; Ohura, Satoshi; Koura, Yoshiaki; Yamauchi, Makoto; Nakajima, Hiroshi; Ueda, Shutaro; Kan, Hiroaki; Anabuki, Naohisa; Nagino, Ryo; Hayashida, Kiyoshi; Tsunemi, Hiroshi; Kohmura, Takayoshi; Ikeda, Shoma; Murakami, Hiroshi; Ozaki, Masanobu; Dotani, Tadayasu; Maeda, Yukie; Sagara, Kenshi

2013-12-01

We report on a proton radiation damage experiment on P-channel CCD newly developed for an X-ray CCD camera onboard the ASTRO-H satellite. The device was exposed up to 109 protons cm-2 at 6.7 MeV. The charge transfer inefficiency (CTI) was measured as a function of radiation dose. In comparison with the CTI currently measured in the CCD camera onboard the Suzaku satellite for 6 years, we confirmed that the new type of P-channel CCD is radiation tolerant enough for space use. We also confirmed that a charge-injection technique and lowering the operating temperature efficiently work to reduce the CTI for our device. A comparison with other P-channel CCD experiments is also discussed. We performed a proton radiation damage experiment on a new P-channel CCD. The device was exposed up to 109 protons cm-2 at 6.7 MeV. We confirmed that it is radiation tolerant enough for space use. We confirmed that a charge-injection technique reduces the CTI. We confirmed that lowering the operating temperature also reduces the CTI.

Evaluating a CCD film digitizer: comparing interpretation accuracy with original film readings

NASA Astrophysics Data System (ADS)

Gitlin, Joseph N.; Scott, William W., Jr.; Bell, Kathryn; Narayan, Anand

2002-04-01

The purpose of the study was to determine if there were differences between the interpretations of radiographic images resulting from digitizing films using a recently developed CCD unit, and the readings of the original films. The general hypothesis to be tested was that there were no significant differences in the measures of accuracy, sensitivity, specificity and ROC analyses when the interpretations related to the two modes were compared. The authors selected 120 radiographic examinations for the study from departmental teaching files, which included chest, abdomen, extremity and other cases that were considered difficult to interpret. The authors also selected six specific abnormalities visualized on 60 of the cases as true positives to classify the reports. After anonymizing the patient identification, the films were digitized and independently interpreted by four board-certified radiologists. Each reader read all of the examinations, half on film alternators and the other half on a high-resolution soft-copy workstation. No reader interpreted the same examination more than once. As of this date, the preliminary results indicate that the hypothesis will be accepted, but more analyses of the data must be performed to confirm the early findings. The additional work will include complete verification of data entry and classification of interpretations, a detailed review of perceived image quality and completion of the ROC analysis by pairs of readers. If the results are confirmed, radiologists, other physicians and administrators will have another reliable option to conventional film practice through increased access to remote primary diagnosis and consultation using high-speed telecommunication media.

Fast auto-acquisition tomography tilt series by using HD video camera in ultra-high voltage electron microscope.

PubMed

Nishi, Ryuji; Cao, Meng; Kanaji, Atsuko; Nishida, Tomoki; Yoshida, Kiyokazu; Isakozawa, Shigeto

2014-11-01

The ultra-high voltage electron microscope (UHVEM) H-3000 with the world highest acceleration voltage of 3 MV can observe remarkable three dimensional microstructures of microns-thick samples[1]. Acquiring a tilt series of electron tomography is laborious work and thus an automatic technique is highly desired. We proposed the Auto-Focus system using image Sharpness (AFS)[2,3] for UHVEM tomography tilt series acquisition. In the method, five images with different defocus values are firstly acquired and the image sharpness are calculated. The sharpness are then fitted to a quasi-Gaussian function to decide the best focus value[3]. Defocused images acquired by the slow scan CCD (SS-CCD) camera (Hitachi F486BK) are of high quality but one minute is taken for acquisition of five defocused images.In this study, we introduce a high-definition video camera (HD video camera; Hamamatsu Photonics K. K. C9721S) for fast acquisition of images[4]. It is an analog camera but the camera image is captured by a PC and the effective image resolution is 1280×1023 pixels. This resolution is lower than that of the SS-CCD camera of 4096×4096 pixels. However, the HD video camera captures one image for only 1/30 second. In exchange for the faster acquisition the S/N of images are low. To improve the S/N, 22 captured frames are integrated so that each image sharpness is enough to become lower fitting error. As countermeasure against low resolution, we selected a large defocus step, which is typically five times of the manual defocus step, to discriminate different defocused images.By using HD video camera for autofocus process, the time consumption for each autofocus procedure was reduced to about six seconds. It took one second for correction of an image position and the total correction time was seven seconds, which was shorter by one order than that using SS-CCD camera. When we used SS-CCD camera for final image capture, it took 30 seconds to record one tilt image. We can obtain a tilt series of 61 images within 30 minutes. Accuracy and repeatability were good enough to practical use (Figure 1). We successfully reduced the total acquisition time of a tomography tilt series in half than before.jmicro;63/suppl_1/i25/DFU066F1F1DFU066F1Fig. 1.Objective lens current change with a tilt angle during acquisition of tomography series (Sample: a rat hepatocyte, thickness: 2 m, magnification: 4k, acc. voltage: 2 MV). Tilt angle range is ±60 degree with 2 degree step angle. Two series were acquired in the same area. Both data were almost same and the deviation was smaller than the minimum step by manual, so auto-focus worked well. We also developed a computer-aided three dimensional (3D) visualization and analysis software for electron tomography "HawkC" which can sectionalize the 3D data semi-automatically[5,6]. If this auto-acquisition system is used with IMOD reconstruction software[7] and HawkC software, we will be able to do on-line UHVEM tomography. The system would help pathology examination in the future.This work was supported by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, under a Grant-in-Aid for Scientific Research (Grant No. 23560024, 23560786), and SENTAN, Japan Science and Technology Agency, Japan. © The Author 2014. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

High-Speed Camera and High-Vision Camera Observations of TLEs from Jet Aircraft in Winter Japan and in Summer US

NASA Astrophysics Data System (ADS)

Sato, M.; Takahashi, Y.; Kudo, T.; Yanagi, Y.; Kobayashi, N.; Yamada, T.; Project, N.; Stenbaek-Nielsen, H. C.; McHarg, M. G.; Haaland, R. K.; Kammae, T.; Cummer, S. A.; Yair, Y.; Lyons, W. A.; Ahrns, J.; Yukman, P.; Warner, T. A.; Sonnenfeld, R. G.; Li, J.; Lu, G.

2011-12-01

The time evolution and spatial distributions of transient luminous events (TLEs) are the key parameters to identify the relationship between TLEs and parent lightning discharges, roles of electromagnetic pulses (EMPs) emitted by horizontal and vertical lightning currents in the formation of TLEs, and the occurrence condition and mechanisms of TLEs. Since the time scales of TLEs is typically less than a few milliseconds, new imaging technique that enable us to capture images with a high time resolution of < 1ms is awaited. By courtesy of "Cosmic Shore" Project conducted by Japan Broadcasting Corporation (NHK), we have carried out optical observations using a high-speed Image-Intensified (II) CMOS camera and a high-vision three-CCD camera from a jet aircraft on November 28 and December 3, 2010 in winter Japan. Using the high-speed II-CMOS camera, it is possible to capture images with 8,300 frames per second (fps), which corresponds to the time resolution of 120 us. Using the high-vision three-CCD camera, it is possible to capture high quality, true color images of TLEs with a 1920x1080 pixel size and with a frame rate of 30 fps. During the two observation flights, we have succeeded to detect 28 sprite events, and 3 elves events totally. In response to this success, we have conducted a combined aircraft and ground-based campaign of TLE observations at the High Plains in summer US. We have installed same NHK high-speed and high-vision cameras in a jet aircraft. In the period from June 27 and July 10, 2011, we have operated aircraft observations in 8 nights, and we have succeeded to capture TLE images for over a hundred events by the high-vision camera and succeeded to acquire over 40 high-speed images simultaneously. At the presentation, we will introduce the outlines of the two aircraft campaigns, and will introduce the characteristics of the time evolution and spatial distributions of TLEs observed in winter Japan, and will show the initial results of high-speed image data analysis of TLEs in summer US.

Study of surface integrity AISI 4140 as result of hard, dry and high speed machining using CBN

NASA Astrophysics Data System (ADS)

Ginting, B.; Sembiring, R. W.; Manurung, N.

2017-09-01

The concept of hard, dry and high speed machining can be combined, to produce high productivity, with lower production costs in manufacturing industry. Hard lathe process can be a solution to reduce production time. In lathe hard alloy steels reported problems relating to the integrity of such surface roughness, residual stress, the white layer and the surface integrity. AISI 4140 material is used for high reliable hydraulic system components. This material includes in cold work tool steel. Consideration election is because this material is able to be hardened up to 55 HRC. In this research, the experimental design using CCD model fit with three factors, each factor is composed of two levels, and six central point, experiments were conducted with 1 replications. The experimental design research using CCD model fit.

CCD correlation techniques

NASA Technical Reports Server (NTRS)

Hewes, C. R.; Bosshart, P. W.; Eversole, W. L.; Dewit, M.; Buss, D. D.

1976-01-01

Two CCD techniques were discussed for performing an N-point sampled data correlation between an input signal and an electronically programmable reference function. The design and experimental performance of an implementation of the direct time correlator utilizing two analog CCDs and MOS multipliers on a single IC were evaluated. The performance of a CCD implementation of the chirp z transform was described, and the design of a new CCD integrated circuit for performing correlation by multiplication in the frequency domain was presented. This chip provides a discrete Fourier transform (DFT) or inverse DFT, multipliers, and complete support circuitry for the CCD CZT. The two correlation techniques are compared.

Improved sensitivity high-definition interline CCD using the KODAK TRUESENSE color filter pattern

NASA Astrophysics Data System (ADS)

DiBella, James; Andreghetti, Marco; Enge, Amy; Chen, William; Stanka, Timothy; Kaser, Robert

2010-01-01

The KODAK TRUESENSE Color Filter Pattern has technology that for the first time is applied to a commercially available interline CCD. This 2/3" true-HD sensor will be described along with its performance attributes, including sensitivity improvement as compared to the Bayer CFA version of the same sensor. In addition, an overview of the system developed for demonstration and evaluation will be provided. Examples of the benefits of the new technology in specific applications including surveillance and intelligent traffic systems will be discussed.

Optical diagnostics of the arc plasma using fast intensified CCD-spectrograph system

NASA Astrophysics Data System (ADS)

Pavelescu, Gabriela; Guillot, Stephane; Braic, Mariana T.; Hong, Dunpin; Pavelescu, D.; Fleurier, Claude; Braic, Viorel; Gherendi, F.; Dumitrescu, G.; Anghelita, P.; Bauchire, J. M.

2004-10-01

Spectroscopic diagnostics, using intensified high speed CCD camera, was applied to study the arc dynamics in low voltage circuit breakers, in vacuum and in air. Time-resolved emission spectroscopy of the vacuum arc plasma, generated during electrode separation, provided information about the interruption process. The investigations were focused on the partial unsuccessful interruption around current zero. Absorption spectroscopy, in a peculiar setup, was used in order to determine the metallic atoms densities in the interelectrode space of a low voltage circuit breaker, working in ambient air.

The STROBE-X Science Case: An Overview

NASA Astrophysics Data System (ADS)

Maccarone, Thomas J.; STROBE-X consortium

2018-01-01

STROBE-X is a proposed NASA Probe class mission aimed at the extremes of high throughput X-ray astronomy, making use of an 8 m^2 total collecting area, CCD-quality spectral resolution, and a state-of-the art wide field monitor with both very large instantaneous sky coverage (ideal for follow-up of LIGO events) and good intrinsic spectral and time resolution. The core goals are time domain astrophysics and high count spectroscopy. Its capabilities span a broad range of topics, including those traditional to X-ray timing missions, like understanding the equation of states of neutron stars, and the spin distributions and masses of neutron stars and stellar mass and supermassive black holes, and the rates, and detailed properties, of a variety of classes of X-ray transients; and also topics not traditionally studied by such missions such as the spectra of supernova remnants, comets and of clusters and groups of galaxies.

High contrast computed tomography with synchrotron radiation

NASA Astrophysics Data System (ADS)

Itai, Yuji; Takeda, Tohoru; Akatsuka, Takao; Maeda, Tomokazu; Hyodo, Kazuyuki; Uchida, Akira; Yuasa, Tetsuya; Kazama, Masahiro; Wu, Jin; Ando, Masami

1995-02-01

This article describes a new monochromatic x-ray CT system using synchrotron radiation with applications in biomedical diagnosis which is currently under development. The system is designed to provide clear images and to detect contrast materials at low concentration for the quantitative functional evaluation of organs in correspondence with their anatomical structures. In this system, with x-ray energy changing from 30 to 52 keV, images can be obtained to detect various contrast materials (iodine, barium, and gadolinium), and K-edge energy subtraction is applied. Herein, the features of the new system designed to enhance the advantages of SR are reported. With the introduction of a double-crystal monochromator, the high-order x-ray contamination is eliminated. The newly designed CCD detector with a wide dynamic range of 60 000:1 has a spatial resolution of 200 μm. The resulting image quality, which is expected to show improved contrast and spatial resolution, is currently under investigation.

A study of ten quasars with redshifts greater than four

NASA Technical Reports Server (NTRS)

Schneider, Donald P.; Schmidt, Maarten; Gunn, James E.

1989-01-01

Four quasars with redshifts greater than four were detected in a low-resolution CCD grism survey. CCD photometry and high S/N, moderate resolution spectra are presented for these quasars and the six other known quasars with redshifts above 4. The M sub B values of nine of the objects are between -27.5 and -25, with the tenth quasar having an M sub B value of -29. The emission lines and shapes of the continua of these ten quasars are similar to those of lower-redshift quasars. The results suggest that the C IV emission lines in high-redshift quasars may be weaker than those in lower-redshift quasars. The continua of all of the high-redshift quasars display strong depressions blueward of the Ly-alpha emission line.

Computer simulation and discussion of high-accuracy laser direction finding in real time

NASA Astrophysics Data System (ADS)

Chen, Wenyi; Chen, Yongzhi

1997-12-01

On condition that CCD is used as the sensor, there are at least five methods that can be used to realize laser's direction finding with high accuracy. They are: image matching method, radiation center method, geometric center method, center of rectangle envelope method and center of maximum run length method. The first three can get the highest accuracy but working in real-time it is too complicated to realize and the cost is very expansive. The other two can also get high accuracy, and it is not difficult to realize working in real time. By using a single-chip microcomputer and an ordinary CCD camera a very simple system can get the position information of a laser beam. The data rate is 50 times per second.

On-Orbit Solar Dynamics Observatory (SDO) Star Tracker Warm Pixel Analysis

NASA Technical Reports Server (NTRS)

Felikson, Denis; Ekinci, Matthew; Hashmall, Joseph A.; Vess, Melissa

2011-01-01

This paper describes the process of identification and analysis of warm pixels in two autonomous star trackers on the Solar Dynamics Observatory (SDO) mission. A brief description of the mission orbit and attitude regimes is discussed and pertinent star tracker hardware specifications are given. Warm pixels are defined and the Quality Index parameter is introduced, which can be explained qualitatively as a manifestation of a possible warm pixel event. A description of the algorithm used to identify warm pixel candidates is given. Finally, analysis of dumps of on-orbit star tracker charge coupled devices (CCD) images is presented and an operational plan going forward is discussed. SDO, launched on February 11, 2010, is operated from the NASA Goddard Space Flight Center (GSFC). SDO is in a geosynchronous orbit with a 28.5 inclination. The nominal mission attitude points the spacecraft X-axis at the Sun, with the spacecraft Z-axis roughly aligned with the Solar North Pole. The spacecraft Y-axis completes the triad. In attitude, SDO moves approximately 0.04 per hour, mostly about the spacecraft Z-axis. The SDO star trackers, manufactured by Galileo Avionica, project the images of stars in their 16.4deg x 16.4deg fields-of-view onto CCD detectors consisting of 512 x 512 pixels. The trackers autonomously identify the star patterns and provide an attitude estimate. Each unit is able to track up to 9 stars. Additionally, each tracker calculates a parameter called the Quality Index, which is a measure of the quality of the attitude solution. Each pixel in the CCD measures the intensity of light and a warns pixel is defined as having a measurement consistently and significantly higher than the mean background intensity level. A warns pixel should also have lower intensity than a pixel containing a star image and will not move across the field of view as the attitude changes (as would a dim star image). It should be noted that the maximum error introduced in the star tracker attitude solution during suspected warm pixel corruptions is within the specified 36 attitude error budget requirement of [35, 70, 70] arcseconds. Thus, the star trackers provided attitude accuracy within the specification for SDO. The star tracker images are intentionally defocused so each star image is detected in more than one CCD pixel. The position of each star is calculated as an intensity-weighted average of the illuminated pixels. The exact method of finding the positions is proprietary to the tracker manufacturer. When a warm pixel happens to be in the vicinity of a star, it can corrupt the calculation of the position of that particular star, thereby corrupting the estimate of the attitude.

Stabilization of the Virulence Plasmid pSLT of Salmonella Typhimurium by Three Maintenance Systems and Its Evaluation by Using a New Stability Test.

PubMed

Lobato-Márquez, Damián; Molina-García, Laura; Moreno-Córdoba, Inma; García-Del Portillo, Francisco; Díaz-Orejas, Ramón

2016-01-01

Certain Salmonella enterica serovars belonging to subspecies I carry low-copy-number virulence plasmids of variable size (50-90 kb). All of these plasmids share the spv operon, which is important for systemic infection. Virulence plasmids are present at low copy numbers. Few copies reduce metabolic burden but suppose a risk of plasmid loss during bacterial division. This drawback is counterbalanced by maintenance modules that ensure plasmid stability, including partition systems and toxin-antitoxin (TA) loci. The low-copy number virulence pSLT plasmid of Salmonella enterica serovar Typhimurium encodes three auxiliary maintenance systems: one partition system ( parAB ) and two TA systems ( ccdAB ST and vapBC2 ST ). The TA module ccdAB ST has previously been shown to contribute to pSLT plasmid stability and vapBC2 ST to bacterial virulence. Here we describe a novel assay to measure plasmid stability based on the selection of plasmid-free cells following elimination of plasmid-containing cells by ParE toxin, a DNA gyrase inhibitor. Using this new maintenance assay we confirmed a crucial role of parAB in pSLT maintenance. We also showed that vapBC2 ST , in addition to contribute to bacterial virulence, is important for plasmid stability. We have previously shown that ccdAB ST encodes an inactive CcdB ST toxin. Using our new stability assay we monitored the contribution to plasmid stability of a ccdAB ST variant containing a single mutation (R99W) that restores the toxicity of CcdB ST . The "activation" of CcdB ST (R99W) did not increase pSLT stability by ccdAB ST . In contrast, ccdAB ST behaves as a canonical type II TA system in terms of transcriptional regulation. Of interest, ccdAB ST was shown to control the expression of a polycistronic operon in the pSLT plasmid. Collectively, these results show that the contribution of the CcdB ST toxin to pSLT plasmid stability may depend on its role as a co-repressor in coordination with CcdA ST antitoxin more than on its toxic activity.

An Efficient Image Compressor for Charge Coupled Devices Camera

PubMed Central

Li, Jin; Xing, Fei; You, Zheng

2014-01-01

Recently, the discrete wavelet transforms- (DWT-) based compressor, such as JPEG2000 and CCSDS-IDC, is widely seen as the state of the art compression scheme for charge coupled devices (CCD) camera. However, CCD images project on the DWT basis to produce a large number of large amplitude high-frequency coefficients because these images have a large number of complex texture and contour information, which are disadvantage for the later coding. In this paper, we proposed a low-complexity posttransform coupled with compressing sensing (PT-CS) compression approach for remote sensing image. First, the DWT is applied to the remote sensing image. Then, a pair base posttransform is applied to the DWT coefficients. The pair base are DCT base and Hadamard base, which can be used on the high and low bit-rate, respectively. The best posttransform is selected by the l p-norm-based approach. The posttransform is considered as the sparse representation stage of CS. The posttransform coefficients are resampled by sensing measurement matrix. Experimental results on on-board CCD camera images show that the proposed approach significantly outperforms the CCSDS-IDC-based coder, and its performance is comparable to that of the JPEG2000 at low bit rate and it does not have the high excessive implementation complexity of JPEG2000. PMID:25114977

A design of optical modulation system with pixel-level modulation accuracy

NASA Astrophysics Data System (ADS)

Zheng, Shiwei; Qu, Xinghua; Feng, Wei; Liang, Baoqiu

2018-01-01

Vision measurement has been widely used in the field of dimensional measurement and surface metrology. However, traditional methods of vision measurement have many limits such as low dynamic range and poor reconfigurability. The optical modulation system before image formation has the advantage of high dynamic range, high accuracy and more flexibility, and the modulation accuracy is the key parameter which determines the accuracy and effectiveness of optical modulation system. In this paper, an optical modulation system with pixel level accuracy is designed and built based on multi-points reflective imaging theory and digital micromirror device (DMD). The system consisted of digital micromirror device, CCD camera and lens. Firstly we achieved accurate pixel-to-pixel correspondence between the DMD mirrors and the CCD pixels by moire fringe and an image processing of sampling and interpolation. Then we built three coordinate systems and calculated the mathematic relationship between the coordinate of digital micro-mirror and CCD pixels using a checkerboard pattern. A verification experiment proves that the correspondence error is less than 0.5 pixel. The results show that the modulation accuracy of system meets the requirements of modulation. Furthermore, the high reflecting edge of a metal circular piece can be detected using the system, which proves the effectiveness of the optical modulation system.

High-performance dual-speed CCD camera system for scientific imaging

NASA Astrophysics Data System (ADS)

Simpson, Raymond W.

1996-03-01

Traditionally, scientific camera systems were partitioned with a `camera head' containing the CCD and its support circuitry and a camera controller, which provided analog to digital conversion, timing, control, computer interfacing, and power. A new, unitized high performance scientific CCD camera with dual speed readout at 1 X 106 or 5 X 106 pixels per second, 12 bit digital gray scale, high performance thermoelectric cooling, and built in composite video output is described. This camera provides all digital, analog, and cooling functions in a single compact unit. The new system incorporates the A/C converter, timing, control and computer interfacing in the camera, with the power supply remaining a separate remote unit. A 100 Mbyte/second serial link transfers data over copper or fiber media to a variety of host computers, including Sun, SGI, SCSI, PCI, EISA, and Apple Macintosh. Having all the digital and analog functions in the camera made it possible to modify this system for the Woods Hole Oceanographic Institution for use on a remote controlled submersible vehicle. The oceanographic version achieves 16 bit dynamic range at 1.5 X 105 pixels/second, can be operated at depths of 3 kilometers, and transfers data to the surface via a real time fiber optic link.

Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney.

PubMed

Li, Yue; Hu, Hongxiang; Butterworth, Michael B; Tian, Jin-Bin; Zhu, Michael X; O'Neil, Roger G

2016-01-01

The voltage- and Ca2+-activated, large conductance K+ channel (BK, maxi-K) is expressed in the collecting duct system of kidney where it underlies flow- and Ca2+-dependent K+ excretion. To determine if other Ca2+-activated K+ channels (KCa) may participate in this process, mouse kidney and the K+-secreting mouse cortical collecting duct (CCD) cell line, mCCDcl1, were assessed for TRPV4 and KCa channel expression and cross-talk. qPCR mRNA analysis and immunocytochemical staining demonstrated TRPV4 and KCa expression in mCCDcl1 cells and kidney connecting tubule (CNT) and CCD. Three subfamilies of KCa channels were revealed: the high Ca2+-binding affinity small-conductance SK channels, SK1and SK3, the intermediate conductance channel, IK1, and the low Ca2+-binding affinity, BK channel (BKα subunit). Apparent expression levels varied in CNT/CCD where analysis of CCD principal cells (PC) and intercalated cells (IC) demonstrated differential staining: SK1:PCIC, IK1:PC>IC, BKα:PC = IC, and TRPV4:PC>IC. Patch clamp analysis and fluorescence Ca2+ imaging of mCCDcl1 cells demonstrated potent TRPV4-mediated Ca2+ entry and strong functional cross-talk between TRPV4 and KCa channels. TRPV4-mediated Ca2+ influx activated each KCa channel, as evidenced by selective inhibition of KCa channels, with each active KCa channel enhancing Ca2+ entry (due to membrane hyperpolarization). Transepithelial electrical resistance (TEER) analysis of confluent mCCDcl1 cells grown on permeable supports further demonstrated this cross-talk where TRPV4 activation induce a decrease in TEER which was partially restored upon selective inhibition of each KCa channel. It is concluded that SK1/SK3 and IK1 are highly expressed along with BKα in CNT and CCD and are closely coupled to TRPV4 activation as observed in mCCDcl1 cells. The data support a model in CNT/CCD segments where strong cross talk between TRPV4-mediated Ca2+ influx and each KCa channel leads to enhance Ca2+ entry which will support activation of the low Ca2+-binding affinity BK channel to promote BK-mediated K+ secretion.

Rethinking Controls on the Long-Term Cenozoic Carbonate Compensation Depth: Case Studies across Late Paleocene - Early Eocene Warming and Late Eocene - Early Oligocene Cooling

NASA Astrophysics Data System (ADS)

Greene, S. E.; Ridgwell, A. J.; Schmidt, D. N.; Kirtland Turner, S.; Paelike, H.; Thomas, E.

2014-12-01

The carbonate compensation depth (CCD) is the depth below which negligible calcium carbonate is preserved in marine sediments. The long-term position of the CCD is often considered to be a powerful constraint on palaeoclimate and atmospheric CO2 concentration due to the requirement that carbonate burial balance riverine weathering over long timescales. The requirement that weathering and burial be in balance is clear, but it is less certain that burial compensates for changes in weathering via shoaling or deepening of the CCD. Because most carbonate burial occurs well above the CCD , changes in weathering fluxes may be primarily accommodated by increasing or decreasing carbonate burial at shallower depths, i.e., at or near the lysocline, the depth range over which carbonate dissolution markedly increases. Indeed, recent earth system modelling studies have suggested that the position of the CCD is relatively insensitive to changes in atmospheric pCO2. Additionally, studies have questioned the nature and strength of the relationship between the CCD, carbonate saturation state in the water column, and lysocline. To test the relationship between palaeoclimate and the location of the CCD, we reconstructed the global, long-term CCD behaviour across major Cenozoic climate transitions: the late Paleocene - early Eocene long-term warming trend (study interval ~58 to 49 Ma) and the late Eocene - early Oligocene cooling and glaciation (study interval ~38 to 27 Ma). We use Earth system modelling (GENIE) to explore the links between atmospheric pCO2 and the CCD, isolating and teasing apart the roles of total dissolved inorganic carbon, temperature, circulation, and productivity in determining the CCD.

CCD detector development projects by the Beamline Technical Support Group at the Advanced Photon Source

NASA Astrophysics Data System (ADS)

Lee, John H.; Fernandez, Patricia; Madden, Tim; Molitsky, Michael; Weizeorick, John

2007-11-01

This paper will describe two ongoing detector projects being developed by the Beamline Technical Support Group at the Advanced Photon Source (APS) at Argonne National Laboratory (ANL). The first project is the design and construction of two detectors: a single-CCD system and a two-by-two Mosaic CCD camera for Small-Angle X-ray Scattering (SAXS). Both of these systems utilize the Kodak KAF-4320E CCD coupled to fiber optic tapers, custom mechanical hardware, electronics, and software developed at ANL. The second project is a Fast-CCD (FCCD) detector being developed in a collaboration between ANL and Lawrence Berkeley National Laboratory (LBNL). This detector will use ANL-designed readout electronics and a custom LBNL-designed CCD, with 480×480 pixels and 96 outputs, giving very fast readout.

The In-flight Spectroscopic Performance of the Swift XRT CCD Camera During 2006-2007

NASA Technical Reports Server (NTRS)

Godet, O.; Beardmore, A.P.; Abbey, A.F.; Osborne, J.P.; Page, K.L.; Evans, P.; Starling, R.; Wells, A.A.; Angelini, L.; Burrows, D.N.;

Design and Fabrication of High-Efficiency CMOS/CCD Imagers

NASA Technical Reports Server (NTRS)

Pain, Bedabrata

2007-01-01

An architecture for back-illuminated complementary metal oxide/semiconductor (CMOS) and charge-coupled-device (CCD) ultraviolet/visible/near infrared- light image sensors, and a method of fabrication to implement the architecture, are undergoing development. The architecture and method are expected to enable realization of the full potential of back-illuminated CMOS/CCD imagers to perform with high efficiency, high sensitivity, excellent angular response, and in-pixel signal processing. The architecture and method are compatible with next-generation CMOS dielectric-forming and metallization techniques, and the process flow of the method is compatible with process flows typical of the manufacture of very-large-scale integrated (VLSI) circuits. The architecture and method overcome all obstacles that have hitherto prevented high-yield, low-cost fabrication of back-illuminated CMOS/CCD imagers by use of standard VLSI fabrication tools and techniques. It is not possible to discuss the obstacles in detail within the space available for this article. Briefly, the obstacles are posed by the problems of generating light-absorbing layers having desired uniform and accurate thicknesses, passivation of surfaces, forming structures for efficient collection of charge carriers, and wafer-scale thinning (in contradistinction to diescale thinning). A basic element of the present architecture and method - the element that, more than any other, makes it possible to overcome the obstacles - is the use of an alternative starting material: Instead of starting with a conventional bulk-CMOS wafer that consists of a p-doped epitaxial silicon layer grown on a heavily-p-doped silicon substrate, one starts with a special silicon-on-insulator (SOI) wafer that consists of a thermal oxide buried between a lightly p- or n-doped, thick silicon layer and a device silicon layer of appropriate thickness and doping. The thick silicon layer is used as a handle: that is, as a mechanical support for the device silicon layer during micro-fabrication.

Characterization of Electrically Active Defects in Si Using CCD Image Sensors

DTIC Science & Technology

1978-02-01

63 35 Dislocation Segments in CCD Imager . . . . . . . . . . . . . 64 36 422 Reflection Topograph of Dislocation Loop ir... Loops . . . . . 3 39 422 Reflection Topograph of Scratch on CCD Imager, . . . 69 40 Dark Current Display of a CCD Imager with 32 ms integration Time...made of each slice using the elon -asoorbio aold developer described in Appendix D. The inagers were then thinned using the procedure at Appendix taor

Mosaic CCD method: A new technique for observing dynamics of cometary magnetospheres

NASA Technical Reports Server (NTRS)

Saito, T.; Takeuchi, H.; Kozuba, Y.; Okamura, S.; Konno, I.; Hamabe, M.; Aoki, T.; Minami, S.; Isobe, S.

1992-01-01

On April 29, 1990, the plasma tail of Comet Austin was observed with a CCD camera on the 105-cm Schmidt telescope at the Kiso Observatory of the University of Tokyo. The area of the CCD used in this observation is only about 1 sq cm. When this CCD is used on the 105-cm Schmidt telescope at the Kiso Observatory, the area corresponds to a narrow square view of 12 ft x 12 ft. By comparison with the photograph of Comet Austin taken by Numazawa (personal communication) on the same night, we see that only a small part of the plasma tail can be photographed at one time with the CCD. However, by shifting the view on the CCD after each exposure, we succeeded in imaging the entire length of the cometary magnetosphere of 1.6 x 10(exp 6) km. This new technique is called 'the mosaic CCD method'. In order to study the dynamics of cometary plasma tails, seven frames of the comet from the head to the tail region were twice imaged with the mosaic CCD method and two sets of images were obtained. Six microstructures, including arcade structures, were identified in both the images. Sketches of the plasma tail including microstructures are included.

Genetic mapping of the cleidocranial dysplasia (CCD) locus on chromosome band 6p21 to include a microdeletion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gelb, B.D.; Desnick, R.J.; Shevell, M.

1995-08-28

Cleidocranial dysplasia (CCD) is a generalized skeletal dysplasia with autosomal dominant inheritance. Recently, the CCD disease locus was localized to 23 and 17 cM regions of chromosome band 6p21 by linkage studies of seven affected families. Of note, the 23 cM region contained a microdeletion detected in one family at D6S459, an interval that was excluded in the 17 cM overlapping region. Here, linkage of CCD to 6p21 was independently confirmed with a maximal two-point LOD score of Z=5.12 with marker D6S452 at {theta}=0.00. Recombinant events in two affected individuals defined a CCD region of 7 cM from D6S465 tomore » D6S282, which overlapped with the CCD region containing the microdeletion but did not overlap with the 17 cM critical region from D6S282 to D6S291. These results suggest the refined localization of the CCD region to 6 cM spanning markers D6S438 to D6S282, thereby reviving the possibility that the CCD gene lies within the microdeletion at D6S459. 13 refs., 2 figs., 1 tab.« less

Development of a driving method suitable for ultrahigh-speed shooting in a 2M-fps 300k-pixel single-chip color camera

NASA Astrophysics Data System (ADS)

Yonai, J.; Arai, T.; Hayashida, T.; Ohtake, H.; Namiki, J.; Yoshida, T.; Etoh, T. Goji

2012-03-01

We have developed an ultrahigh-speed CCD camera that can capture instantaneous phenomena not visible to the human eye and impossible to capture with a regular video camera. The ultrahigh-speed CCD was specially constructed so that the CCD memory between the photodiode and the vertical transfer path of each pixel can store 144 frames each. For every one-frame shot, the electric charges generated from the photodiodes are transferred in one step to the memory of all the parallel pixels, making ultrahigh-speed shooting possible. Earlier, we experimentally manufactured a 1M-fps ultrahigh-speed camera and tested it for broadcasting applications. Through those tests, we learned that there are cases that require shooting speeds (frame rate) of more than 1M fps; hence we aimed to develop a new ultrahigh-speed camera that will enable much faster shooting speeds than what is currently possible. Since shooting at speeds of more than 200,000 fps results in decreased image quality and abrupt heating of the image sensor and drive circuit board, faster speeds cannot be achieved merely by increasing the drive frequency. We therefore had to improve the image sensor wiring layout and the driving method to develop a new 2M-fps, 300k-pixel ultrahigh-speed single-chip color camera for broadcasting purposes.

Real-time tricolor phase measuring profilometry based on CCD sensitivity calibration

NASA Astrophysics Data System (ADS)

Zhu, Lin; Cao, Yiping; He, Dawu; Chen, Cheng

2017-02-01

A real-time tricolor phase measuring profilometry (RTPMP) based on charge coupled device (CCD) sensitivity calibration is proposed. Only one colour fringe pattern whose red (R), green (G) and blue (B) components are, respectively, coded as three sinusoidal phase-shifting gratings with an equivalent shifting phase of 2π/3 is needed and sent to an appointed flash memory on a specialized digital light projector (SDLP). A specialized time-division multiplexing timing sequence actively controls the SDLP to project the fringe patterns in R, G and B channels sequentially onto the measured object in one over seventy-two of a second and meanwhile actively controls a high frame rate monochrome CCD camera to capture the corresponding deformed patterns synchronously with the SDLP. So the sufficient information for reconstructing the three-dimensional (3D) shape in one over twenty-four of a second is obtained. Due to the different spectral sensitivity of the CCD camera to RGB lights, the captured deformed patterns from R, G and B channels cannot share the same peak and valley, which will lead to lower accuracy or even failing to reconstruct the 3D shape. So a deformed pattern amending method based on CCD sensitivity calibration is developed to guarantee the accurate 3D reconstruction. The experimental results verify the feasibility of the proposed RTPMP method. The proposed RTPMP method can obtain the 3D shape at over the video frame rate of 24 frames per second, avoid the colour crosstalk completely and be effective for measuring real-time changing object.

Application of CCD drift-scan photoelectric technique on monitoring GEO satellites

NASA Astrophysics Data System (ADS)

Yu, Yong; Zhao, Xiao-Fen; Luo, Hao; Mao, Yin-Dun; Tang, Zheng-Hong

2018-05-01

Geosynchronous Earth Orbit (GEO) satellites are widely used because of their unique characteristics of high-orbit and remaining permanently in the same area of the sky. Precise monitoring of GEO satellites can provide a key reference for the judgment of satellite operation status, the capture and identification of targets, and the analysis of collision warning. The observation using ground-based optical telescopes plays an important role in the field of monitoring GEO targets. Different from distant celestial bodies, there is a relative movement between the GEO target and the background reference stars, which makes the conventional observation method limited for long focal length telescopes. CCD drift-scan photoelectric technique is applied on monitoring GEO targets. In the case of parking the telescope, the good round images of the background reference stars and the GEO target at the same sky region can be obtained through the alternating observation of CCD drift-scan mode and CCD stare mode, so as to improve the precision of celestial positioning for the GEO target. Observation experiments of GEO targets were carried out with 1.56-meter telescope of Shanghai Astronomical Observatory. The results show that the application of CCD drift-scan photoelectric technique makes the precision of observing the GEO target reach the level of 0.2″, which gives full play to the advantage of the long focal length of the telescope. The effect of orbit improvement based on multi-pass of observations is obvious and the prediction precision of extrapolating to 72-h is in the order of several arc seconds in azimuth and elevation.

CCD radiation damage in ESA Cosmic Visions missions: assessment and mitigation

NASA Astrophysics Data System (ADS)

Lumb, David H.

2009-08-01

Charge Coupled Device (CCD) imagers have been widely used in space-borne astronomical instruments. A frequent concern has been the radiation damage effects on the CCD charge transfer properties. We review some methods for assessing the Charge Transfer Inefficiency (CTI) in CCDs. Techniques to minimise degradation using background charge injection and p-channel CCD architectures are discussed. A critical review of the claims for p-channel architectures is presented. The performance advantage for p-channel CCD performance is shown to be lower than claimed previously. Finally we present some projections for the performance in the context of some future ESA missions.

Targeting excited states in all-trans polyenes with electron-pair states.

PubMed

Boguslawski, Katharina

2016-12-21

Wavefunctions restricted to electron pair states are promising models for strongly correlated systems. Specifically, the pair Coupled Cluster Doubles (pCCD) ansatz allows us to accurately describe bond dissociation processes and heavy-element containing compounds with multiple quasi-degenerate single-particle states. Here, we extend the pCCD method to model excited states using the equation of motion (EOM) formalism. As the cluster operator of pCCD is restricted to electron-pair excitations, EOM-pCCD allows us to target excited electron-pair states only. To model singly excited states within EOM-pCCD, we modify the configuration interaction ansatz of EOM-pCCD to contain also single excitations. Our proposed model represents a simple and cost-effective alternative to conventional EOM-CC methods to study singly excited electronic states. The performance of the excited state models is assessed against the lowest-lying excited states of the uranyl cation and the two lowest-lying excited states of all-trans polyenes. Our numerical results suggest that EOM-pCCD including single excitations is a good starting point to target singly excited states.

New light-amplifier-based detector designs for high spatial resolution and high sensitivity CBCT mammography and fluoroscopy

PubMed Central

Rudin, Stephen; Kuhls, Andrew T.; Yadava, Girijesh K.; Josan, Gaurav C.; Wu, Ye; Chityala, Ravishankar N.; Rangwala, Hussain S.; Ciprian Ionita, N.; Hoffmann, Kenneth R.; Bednarek, Daniel R.

2011-01-01

New cone-beam computed tomographic (CBCT) mammography system designs are presented where the detectors provide high spatial resolution, high sensitivity, low noise, wide dynamic range, negligible lag and high frame rates similar to features required for high performance fluoroscopy detectors. The x-ray detectors consist of a phosphor coupled by a fiber-optic taper to either a high gain image light amplifier (LA) then CCD camera or to an electron multiplying CCD. When a square-array of such detectors is used, a field-of-view (FOV) to 20 × 20 cm can be obtained where the images have pixel-resolution of 100 µm or better. To achieve practical CBCT mammography scan-times, 30 fps may be acquired with quantum limited (noise free) performance below 0.2 µR detector exposure per frame. Because of the flexible voltage controlled gain of the LA’s and EMCCDs, large detector dynamic range is also achievable. Features of such detector systems with arrays of either generation 2 (Gen 2) or 3 (Gen 3) LAs optically coupled to CCD cameras or arrays of EMCCDs coupled directly are compared. Quantum accounting analysis is done for a variety of such designs where either the lowest number of information carriers off the LA photo-cathode or electrons released in the EMCCDs per x-ray absorbed in the phosphor are large enough to imply no quantum sink for the design. These new LA- or EMCCD-based systems could lead to vastly improved CBCT mammography, ROI-CT, or fluoroscopy performance compared to systems using flat panels. PMID:21297904

New light-amplifier-based detector designs for high spatial resolution and high sensitivity CBCT mammography and fluoroscopy

NASA Astrophysics Data System (ADS)

Rudin, Stephen; Kuhls, Andrew T.; Yadava, Girijesh K.; Josan, Gaurav C.; Wu, Ye; Chityala, Ravishankar N.; Rangwala, Hussain S.; Ionita, N. Ciprian; Hoffmann, Kenneth R.; Bednarek, Daniel R.

2006-03-01

New cone-beam computed tomographic (CBCT) mammography system designs are presented where the detectors provide high spatial resolution, high sensitivity, low noise, wide dynamic range, negligible lag and high frame rates similar to features required for high performance fluoroscopy detectors. The x-ray detectors consist of a phosphor coupled by a fiber-optic taper to either a high gain image light amplifier (LA) then CCD camera or to an electron multiplying CCD. When a square-array of such detectors is used, a field-of-view (FOV) to 20 x 20 cm can be obtained where the images have pixel-resolution of 100 μm or better. To achieve practical CBCT mammography scan-times, 30 fps may be acquired with quantum limited (noise free) performance below 0.2 μR detector exposure per frame. Because of the flexible voltage controlled gain of the LA's and EMCCDs, large detector dynamic range is also achievable. Features of such detector systems with arrays of either generation 2 (Gen 2) or 3 (Gen 3) LAs optically coupled to CCD cameras or arrays of EMCCDs coupled directly are compared. Quantum accounting analysis is done for a variety of such designs where either the lowest number of information carriers off the LA photo-cathode or electrons released in the EMCCDs per x-ray absorbed in the phosphor are large enough to imply no quantum sink for the design. These new LA- or EMCCD-based systems could lead to vastly improved CBCT mammography, ROI-CT, or fluoroscopy performance compared to systems using flat panels.

Research on detecting heterogeneous fibre from cotton based on linear CCD camera

NASA Astrophysics Data System (ADS)

Zhang, Xian-bin; Cao, Bing; Zhang, Xin-peng; Shi, Wei

2009-07-01

The heterogeneous fibre in cotton make a great impact on production of cotton textile, it will have a bad effect on the quality of product, thereby affect economic benefits and market competitive ability of corporation. So the detecting and eliminating of heterogeneous fibre is particular important to improve machining technics of cotton, advance the quality of cotton textile and reduce production cost. There are favorable market value and future development for this technology. An optical detecting system obtains the widespread application. In this system, we use a linear CCD camera to scan the running cotton, then the video signals are put into computer and processed according to the difference of grayscale, if there is heterogeneous fibre in cotton, the computer will send an order to drive the gas nozzle to eliminate the heterogeneous fibre. In the paper, we adopt monochrome LED array as the new detecting light source, it's lamp flicker, stability of luminous intensity, lumens depreciation and useful life are all superior to fluorescence light. We analyse the reflection spectrum of cotton and various heterogeneous fibre first, then select appropriate frequency of the light source, we finally adopt violet LED array as the new detecting light source. The whole hardware structure and software design are introduced in this paper.

Design and DSP implementation of star image acquisition and star point fast acquiring and tracking

NASA Astrophysics Data System (ADS)

Zhou, Guohui; Wang, Xiaodong; Hao, Zhihang

2006-02-01

Star sensor is a special high accuracy photoelectric sensor. Attitude acquisition time is an important function index of star sensor. In this paper, the design target is to acquire 10 samples per second dynamic performance. On the basis of analyzing CCD signals timing and star image processing, a new design and a special parallel architecture for improving star image processing are presented in this paper. In the design, the operation moving the data in expanded windows including the star to the on-chip memory of DSP is arranged in the invalid period of CCD frame signal. During the CCD saving the star image to memory, DSP processes the data in the on-chip memory. This parallelism greatly improves the efficiency of processing. The scheme proposed here results in enormous savings of memory normally required. In the scheme, DSP HOLD mode and CPLD technology are used to make a shared memory between CCD and DSP. The efficiency of processing is discussed in numerical tests. Only in 3.5ms is acquired the five lightest stars in the star acquisition stage. In 43us, the data in five expanded windows including stars are moved into the internal memory of DSP, and in 1.6ms, five star coordinates are achieved in the star tracking stage.

Stromvil Photometry of Clusters II. The Open Cluster M67

NASA Astrophysics Data System (ADS)

Boyle, R. P.; Philip, A. G. D.; Straižys, V.

1998-12-01

Use of the Stromgren four-color photometric system with the addition of three filters (P, Z, S) of the Vilnius system allows classifying all the types of stars, even in the presence of significant reddening by interstellar dust (Strai\\v{z}ys et al, 1996). With this Stromvil system we have begun a long-term observational program on the Vatican Advanced Technology Telescope (VATT) on Mt. Graham, Arizona, (see Philip et al,1996). The Loral 2K by 2K CCD, prepared by M. Lesser of the University of Arizona CCD Lab, gives as high as 90% quantum efficiency in most of the filters and notably 65% at the u filter. To demonstrate the degree of success so far attained in this CCD Stromvil System, we present preliminary results on the open cluster M67. We will compare some of our photometry with the CCD results of B. A. Twarog (1987) and the photoelectric photometry of Nissen et al. (1987). REFERENCES Nissen, P.E., Twarog, B.A., and Crawford, D.L. 1987, A.J. 93,634 Philip, A.G. Davis, Boyle, R.P., Strai\\v{z}ys, V. 1996, Baltic Astronomy,5,445 Strai\\v{z}ys, V., Crawford, D.L., Philip, A.G.Davis 1996, Baltic Astronomy, 5,83 Twarog, B.A. 1987, A.J. 93,647

Digital holographic interferometry applied to the investigation of ignition process.

PubMed

Pérez-Huerta, J S; Saucedo-Anaya, Tonatiuh; Moreno, I; Ariza-Flores, D; Saucedo-Orozco, B

2017-06-12

We use the digital holographic interferometry (DHI) technique to display the early ignition process for a butane-air mixture flame. Because such an event occurs in a short time (few milliseconds), a fast CCD camera is used to study the event. As more detail is required for monitoring the temporal evolution of the process, less light coming from the combustion is captured by the CCD camera, resulting in a deficient and underexposed image. Therefore, the CCD's direct observation of the combustion process is limited (down to 1000 frames per second). To overcome this drawback, we propose the use of DHI along with a high power laser in order to supply enough light to increase the speed capture, thus improving the visualization of the phenomenon in the initial moments. An experimental optical setup based on DHI is used to obtain a large sequence of phase maps that allows us to observe two transitory stages in the ignition process: a first explosion which slightly emits visible light, and a second stage induced by variations in temperature when the flame is emerging. While the last stage can be directly monitored by the CCD camera, the first stage is hardly detected by direct observation, and DHI clearly evidences this process. Furthermore, our method can be easily adapted for visualizing other types of fast processes.

CFCCD Manual | CTIO

Science.gov Websites

DECam SAM 0.9-m CCD Goodman SOI Optical Spectrographs CHIRON COSMOS Goodman Filters Telescopes Blanco 4 4.4.4 Gain 4.5: CCD scales at various foci APPENDIX I: Filters for CCD Imaging II: Gain and Readout

Lack of evidence for an association between Iridovirus and colony collapse disorder.

PubMed

Tokarz, Rafal; Firth, Cadhla; Street, Craig; Cox-Foster, Diana L; Lipkin, W Ian

2011-01-01

Colony collapse disorder (CCD) is characterized by the unexplained losses of large numbers of adult worker bees (Apis mellifera) from apparently healthy colonies. Although infections, toxins, and other stressors have been associated with the onset of CCD, the pathogenesis of this disorder remains obscure. Recently, a proteomics study implicated a double-stranded DNA virus, invertebrate iridescent virus (Family Iridoviridae) along with a microsporidium (Nosema sp.) as the cause of CCD. We tested the validity of this relationship using two independent methods: (i) we surveyed healthy and CCD colonies from the United States and Israel for the presence of members of the Iridovirus genus and (ii) we reanalyzed metagenomics data previously generated from RNA pools of CCD colonies for the presence of Iridovirus-like sequences. Neither analysis revealed any evidence to suggest the presence of an Iridovirus in healthy or CCD colonies.

Lack of Evidence for an Association between Iridovirus and Colony Collapse Disorder

PubMed Central

Street, Craig; Cox-Foster, Diana L.; Lipkin, W. Ian

2011-01-01

Colony collapse disorder (CCD) is characterized by the unexplained losses of large numbers of adult worker bees (Apis mellifera) from apparently healthy colonies. Although infections, toxins, and other stressors have been associated with the onset of CCD, the pathogenesis of this disorder remains obscure. Recently, a proteomics study implicated a double-stranded DNA virus, invertebrate iridescent virus (Family Iridoviridae) along with a microsporidium (Nosema sp.) as the cause of CCD. We tested the validity of this relationship using two independent methods: (i) we surveyed healthy and CCD colonies from the United States and Israel for the presence of members of the Iridovirus genus and (ii) we reanalyzed metagenomics data previously generated from RNA pools of CCD colonies for the presence of Iridovirus-like sequences. Neither analysis revealed any evidence to suggest the presence of an Iridovirus in healthy or CCD colonies. PMID:21738798

Signal processing applications of massively parallel charge domain computing devices

NASA Technical Reports Server (NTRS)

Fijany, Amir (Inventor); Barhen, Jacob (Inventor); Toomarian, Nikzad (Inventor)

1999-01-01

The present invention is embodied in a charge coupled device (CCD)/charge injection device (CID) architecture capable of performing a Fourier transform by simultaneous matrix vector multiplication (MVM) operations in respective plural CCD/CID arrays in parallel in O(1) steps. For example, in one embodiment, a first CCD/CID array stores charge packets representing a first matrix operator based upon permutations of a Hartley transform and computes the Fourier transform of an incoming vector. A second CCD/CID array stores charge packets representing a second matrix operator based upon different permutations of a Hartley transform and computes the Fourier transform of an incoming vector. The incoming vector is applied to the inputs of the two CCD/CID arrays simultaneously, and the real and imaginary parts of the Fourier transform are produced simultaneously in the time required to perform a single MVM operation in a CCD/CID array.

[Digital thoracic radiology: devices, image processing, limits].

PubMed

Frija, J; de Géry, S; Lallouet, F; Guermazi, A; Zagdanski, A M; De Kerviler, E

2001-09-01

In a first part, the different techniques of digital thoracic radiography are described. Since computed radiography with phosphore plates are the most commercialized it is more emphasized. But the other detectors are also described, as the drum coated with selenium and the direct digital radiography with selenium detectors. The other detectors are also studied in particular indirect flat panels detectors and the system with four high resolution CCD cameras. In a second step the most important image processing are discussed: the gradation curves, the unsharp mask processing, the system MUSICA, the dynamic range compression or reduction, the soustraction with dual energy. In the last part the advantages and the drawbacks of computed thoracic radiography are emphasized. The most important are the almost constant good quality of the pictures and the possibilities of image processing.

Smart photonic coating for civil engineering field: for a future inspection technology on concrete bridge

NASA Astrophysics Data System (ADS)

Fudouzi, Hiroshi; Tsuchiya, Koichi; Todoroki, Shin-ichi; Hyakutake, Tsuyoshi; Nitta, Hiroyuki; Nishizaki, Itaru; Tanaka, Yoshikazu; Ohya, Takao

2017-04-01

Here we will propose the conceptual new idea of the inspection of concrete bridge using smart materials and mobile IoT system. We apply opal photonic crystal film to detect cracks on concrete infrastructures. High quality opal photonic crystal films were coated on black color PET sheet over 1000 cm2 area. The opal film sheet was cut and adhered to concrete or mortar test pieces by epoxy resin. In the tensile test, the structural color of the opal sheet was changed when the crack was formed. As a demonstration, we have installated the opal film sheet on the wall of the concrete bridge. Our final purpose is the color change will be recorded by portable CCD devices, and send to expert via IoT network.

Design and realization of an AEC&AGC system for the CCD aerial camera

NASA Astrophysics Data System (ADS)

Liu, Hai ying; Feng, Bing; Wang, Peng; Li, Yan; Wei, Hao yun

2015-08-01

An AEC and AGC(Automatic Exposure Control and Automatic Gain Control) system was designed for a CCD aerial camera with fixed aperture and electronic shutter. The normal AEC and AGE algorithm is not suitable to the aerial camera since the camera always takes high-resolution photographs in high-speed moving. The AEC and AGE system adjusts electronic shutter and camera gain automatically according to the target brightness and the moving speed of the aircraft. An automatic Gamma correction is used before the image is output so that the image is better for watching and analyzing by human eyes. The AEC and AGC system could avoid underexposure, overexposure, or image blurring caused by fast moving or environment vibration. A series of tests proved that the system meet the requirements of the camera system with its fast adjusting speed, high adaptability, high reliability in severe complex environment.

An assessment of a film enhancement system for use in a radiation therapy department.

PubMed

Solowsky, E L; Reinstein, L E; Meek, A G

1990-01-01

The clinical uses of a radiotherapy film enhancement system are explored. The primary functions of the system are to improve the quality of poorly exposed simulator and portal films, and to perform comparisons between the two films to determine whether patient or block positioning errors are present. Other features include: the production of inexpensive, high quality hardcopy images of simulation films and initial portal films for chart documentation, the capacity to overlay lateral simulation films with sagittal MRI films to aid in field design, and a mode to zoom in on individual CT or MRI images and enlarge them for video display during chart rounds or instructional sessions. This commercially available system is comprised of a microcomputer, frame grabber, CCD camera with zoom lens, and a high-resolution thermal printer. The user-friendly software is menu driven and utilizes both keyboard and track ball to perform its functions. At the heart of the software is a very fast Adaptive Histogram Equalization (AHE) routine, which enhances and improves the readability of most portal films. The system has been evaluated for several disease sites, and its advantages and limitations will be presented.

High-speed potato grading and quality inspection based on a color vision system

NASA Astrophysics Data System (ADS)

Noordam, Jacco C.; Otten, Gerwoud W.; Timmermans, Toine J. M.; van Zwol, Bauke H.

2000-03-01

A high-speed machine vision system for the quality inspection and grading of potatoes has been developed. The vision system grades potatoes on size, shape and external defects such as greening, mechanical damages, rhizoctonia, silver scab, common scab, cracks and growth cracks. A 3-CCD line-scan camera inspects the potatoes in flight as they pass under the camera. The use of mirrors to obtain a 360-degree view of the potato and the lack of product holders guarantee a full view of the potato. To achieve the required capacity of 12 tons/hour, 11 SHARC Digital Signal Processors perform the image processing and classification tasks. The total capacity of the system is about 50 potatoes/sec. The color segmentation procedure uses Linear Discriminant Analysis (LDA) in combination with a Mahalanobis distance classifier to classify the pixels. The procedure for the detection of misshapen potatoes uses a Fourier based shape classification technique. Features such as area, eccentricity and central moments are used to discriminate between similar colored defects. Experiments with red and yellow skin-colored potatoes have shown that the system is robust and consistent in its classification.

The future scientific CCD

NASA Technical Reports Server (NTRS)

Janesick, J. R.; Elliott, T.; Collins, S.; Marsh, H.; Blouke, M. M.

1984-01-01

Since the first introduction of charge-coupled devices (CCDs) in 1970, CCDs have been considered for applications related to memories, logic circuits, and the detection of visible radiation. It is pointed out, however, that the mass market orientation of CCD development has left largely untapped the enormous potential of these devices for advanced scientific instrumentation. The present paper has, therefore, the objective to introduce the CCD characteristics to the scientific community, taking into account prospects for further improvement. Attention is given to evaluation criteria, a summary of current CCDs, CCD performance characteristics, absolute calibration tools, quantum efficiency, aspects of charge collection, charge transfer efficiency, read noise, and predictions regarding the characteristics of the next generation of silicon scientific CCD imagers.

Enhanced performance CCD output amplifier

DOEpatents

Dunham, Mark E.; Morley, David W.

1996-01-01

A low-noise FET amplifier is connected to amplify output charge from a che coupled device (CCD). The FET has its gate connected to the CCD in common source configuration for receiving the output charge signal from the CCD and output an intermediate signal at a drain of the FET. An intermediate amplifier is connected to the drain of the FET for receiving the intermediate signal and outputting a low-noise signal functionally related to the output charge signal from the CCD. The amplifier is preferably connected as a virtual ground to the FET drain. The inherent shunt capacitance of the FET is selected to be at least equal to the sum of the remaining capacitances.

Scientific CCD technology at JPL

NASA Technical Reports Server (NTRS)

Janesick, J.; Collins, S. A.; Fossum, E. R.

1991-01-01

Charge-coupled devices (CCD's) were recognized for their potential as an imaging technology almost immediately following their conception in 1970. Twenty years later, they are firmly established as the technology of choice for visible imaging. While consumer applications of CCD's, especially the emerging home video camera market, dominated manufacturing activity, the scientific market for CCD imagers has become significant. Activity of the Jet Propulsion Laboratory and its industrial partners in the area of CCD imagers for space scientific instruments is described. Requirements for scientific imagers are significantly different from those needed for home video cameras, and are described. An imager for an instrument on the CRAF/Cassini mission is described in detail to highlight achieved levels of performance.

Chromatic Modulator for High Resolution CCD or APS Devices

NASA Technical Reports Server (NTRS)

Hartley, Frank T. (Inventor); Hull, Anthony B. (Inventor)

2003-01-01

A system for providing high-resolution color separation in electronic imaging. Comb drives controllably oscillate a red-green-blue (RGB) color strip filter system (or otherwise) over an electronic imaging system such as a charge-coupled device (CCD) or active pixel sensor (APS). The color filter is modulated over the imaging array at a rate three or more times the frame rate of the imaging array. In so doing, the underlying active imaging elements are then able to detect separate color-separated images, which are then combined to provide a color-accurate frame which is then recorded as the representation of the recorded image. High pixel resolution is maintained. Registration is obtained between the color strip filter and the underlying imaging array through the use of electrostatic comb drives in conjunction with a spring suspension system.

Colony Collapse Disorder: A Descriptive Study

PubMed Central

vanEngelsdorp, Dennis; Evans, Jay D.; Saegerman, Claude; Mullin, Chris; Haubruge, Eric; Nguyen, Bach Kim; Frazier, Maryann; Frazier, Jim; Cox-Foster, Diana; Chen, Yanping; Underwood, Robyn; Tarpy, David R.; Pettis, Jeffery S.

2009-01-01

Background Over the last two winters, there have been large-scale, unexplained losses of managed honey bee (Apis mellifera L.) colonies in the United States. In the absence of a known cause, this syndrome was named Colony Collapse Disorder (CCD) because the main trait was a rapid loss of adult worker bees. We initiated a descriptive epizootiological study in order to better characterize CCD and compare risk factor exposure between populations afflicted by and not afflicted by CCD. Methods and Principal Findings Of 61 quantified variables (including adult bee physiology, pathogen loads, and pesticide levels), no single measure emerged as a most-likely cause of CCD. Bees in CCD colonies had higher pathogen loads and were co-infected with a greater number of pathogens than control populations, suggesting either an increased exposure to pathogens or a reduced resistance of bees toward pathogens. Levels of the synthetic acaricide coumaphos (used by beekeepers to control the parasitic mite Varroa destructor) were higher in control colonies than CCD-affected colonies. Conclusions/Significance This is the first comprehensive survey of CCD-affected bee populations that suggests CCD involves an interaction between pathogens and other stress factors. We present evidence that this condition is contagious or the result of exposure to a common risk factor. Potentially important areas for future hypothesis-driven research, including the possible legacy effect of mite parasitism and the role of honey bee resistance to pesticides, are highlighted. PMID:19649264

Colony collapse disorder: a descriptive study.

PubMed

Vanengelsdorp, Dennis; Evans, Jay D; Saegerman, Claude; Mullin, Chris; Haubruge, Eric; Nguyen, Bach Kim; Frazier, Maryann; Frazier, Jim; Cox-Foster, Diana; Chen, Yanping; Underwood, Robyn; Tarpy, David R; Pettis, Jeffery S

2009-08-03

Over the last two winters, there have been large-scale, unexplained losses of managed honey bee (Apis mellifera L.) colonies in the United States. In the absence of a known cause, this syndrome was named Colony Collapse Disorder (CCD) because the main trait was a rapid loss of adult worker bees. We initiated a descriptive epizootiological study in order to better characterize CCD and compare risk factor exposure between populations afflicted by and not afflicted by CCD. Of 61 quantified variables (including adult bee physiology, pathogen loads, and pesticide levels), no single measure emerged as a most-likely cause of CCD. Bees in CCD colonies had higher pathogen loads and were co-infected with a greater number of pathogens than control populations, suggesting either an increased exposure to pathogens or a reduced resistance of bees toward pathogens. Levels of the synthetic acaricide coumaphos (used by beekeepers to control the parasitic mite Varroa destructor) were higher in control colonies than CCD-affected colonies. This is the first comprehensive survey of CCD-affected bee populations that suggests CCD involves an interaction between pathogens and other stress factors. We present evidence that this condition is contagious or the result of exposure to a common risk factor. Potentially important areas for future hypothesis-driven research, including the possible legacy effect of mite parasitism and the role of honey bee resistance to pesticides, are highlighted.

Cryostat and CCD for MEGARA at GTC

NASA Astrophysics Data System (ADS)

Castillo-Domínguez, E.; Ferrusca, D.; Tulloch, S.; Velázquez, M.; Carrasco, E.; Gallego, J.; Gil de Paz, A.; Sánchez, F. M.; Vílchez Medina, J. M.

2012-09-01

MEGARA (Multi-Espectrógrafo en GTC de Alta Resolución para Astronomía) is the new integral field unit (IFU) and multi-object spectrograph (MOS) instrument for the GTC. The spectrograph subsystems include the pseudo-slit, the shutter, the collimator with a focusing mechanism, pupil elements on a volume phase holographic grating (VPH) wheel and the camera joined to the cryostat through the last lens, with a CCD detector inside. In this paper we describe the full preliminary design of the cryostat which will harbor the CCD detector for the spectrograph. The selected cryogenic device is an LN2 open-cycle cryostat which has been designed by the "Astronomical Instrumentation Lab for Millimeter Wavelengths" at INAOE. A complete description of the cryostat main body and CCD head is presented as well as all the vacuum and temperature sub-systems to operate it. The CCD is surrounded by a radiation shield to improve its performance and is placed in a custom made mechanical mounting which will allow physical adjustments for alignment with the spectrograph camera. The 4k x 4k pixel CCD231 is our selection for the cryogenically cooled detector of MEGARA. The characteristics of this CCD, the internal cryostat cabling and CCD controller hardware are discussed. Finally, static structural finite element modeling and thermal analysis results are shown to validate the cryostat model.

Robotic CCD microscope for enhanced crystal recognition

DOEpatents

Segelke, Brent W.; Toppani, Dominique

2007-11-06

A robotic CCD microscope and procedures to automate crystal recognition. The robotic CCD microscope and procedures enables more accurate crystal recognition, leading to fewer false negative and fewer false positives, and enable detection of smaller crystals compared to other methods available today.

Optical CT scanning of PRESAGETM polyurethane samples with a CCD-based readout system

NASA Astrophysics Data System (ADS)

Doran, S. J.; Krstajic, N.; Adamovics, J.; Jenneson, P. M.

2004-01-01

This article demonstrates the resolution capabilities of the CCD scanner under ideal circumstances and describes the first CCD-based optical CT experiments on a new class of dosimeter, known as PRESAGETM (Heuris Pharma, Skillman, NJ).

A dual communicator and dual grid-resolution algorithm for petascale simulations of turbulent mixing at high Schmidt number

NASA Astrophysics Data System (ADS)

Clay, M. P.; Buaria, D.; Gotoh, T.; Yeung, P. K.

2017-10-01

A new dual-communicator algorithm with very favorable performance characteristics has been developed for direct numerical simulation (DNS) of turbulent mixing of a passive scalar governed by an advection-diffusion equation. We focus on the regime of high Schmidt number (S c), where because of low molecular diffusivity the grid-resolution requirements for the scalar field are stricter than those for the velocity field by a factor √{ S c }. Computational throughput is improved by simulating the velocity field on a coarse grid of Nv3 points with a Fourier pseudo-spectral (FPS) method, while the passive scalar is simulated on a fine grid of Nθ3 points with a combined compact finite difference (CCD) scheme which computes first and second derivatives at eighth-order accuracy. A static three-dimensional domain decomposition and a parallel solution algorithm for the CCD scheme are used to avoid the heavy communication cost of memory transposes. A kernel is used to evaluate several approaches to optimize the performance of the CCD routines, which account for 60% of the overall simulation cost. On the petascale supercomputer Blue Waters at the University of Illinois, Urbana-Champaign, scalability is improved substantially with a hybrid MPI-OpenMP approach in which a dedicated thread per NUMA domain overlaps communication calls with computational tasks performed by a separate team of threads spawned using OpenMP nested parallelism. At a target production problem size of 81923 (0.5 trillion) grid points on 262,144 cores, CCD timings are reduced by 34% compared to a pure-MPI implementation. Timings for 163843 (4 trillion) grid points on 524,288 cores encouragingly maintain scalability greater than 90%, although the wall clock time is too high for production runs at this size. Performance monitoring with CrayPat for problem sizes up to 40963 shows that the CCD routines can achieve nearly 6% of the peak flop rate. The new DNS code is built upon two existing FPS and CCD codes. With the grid ratio Nθ /Nv = 8, the disparity in the computational requirements for the velocity and scalar problems is addressed by splitting the global communicator MPI_COMM_WORLD into disjoint communicators for the velocity and scalar fields, respectively. Inter-communicator transfer of the velocity field from the velocity communicator to the scalar communicator is handled with discrete send and non-blocking receive calls, which are overlapped with other operations on the scalar communicator. For production simulations at Nθ = 8192 and Nv = 1024 on 262,144 cores for the scalar field, the DNS code achieves 94% strong scaling relative to 65,536 cores and 92% weak scaling relative to Nθ = 1024 and Nv = 128 on 512 cores.

Ultrahigh resolution protein crystallography: Concanavalin A to 0.94 Å and beyond

NASA Astrophysics Data System (ADS)

Deacon, A. M.; Gleichmann, T.; Harrop, S. J.; Helliwell, J. R.; Kalb Gilboa, A. J.; Yariv, J.

1996-09-01

Many years ago the idea of collecting voluminous quantities of weak reflection intensities from a protein crystal, at high resolution, was a particular challenge [J.R. Helliwell (1979) Daresbury Study Weekend DL/SCI R13, pp. 1-6]. The combination of insertion devices with very high x-ray fluxes at short x-ray wavelengths, sensitive CCD detectors, and freezing of crystals have provided the means to certainly match those best hopes. So much so that the data can best be described as ultrahigh resolution, at least as evidenced in our studies of the 25000 molecular weight plant protein concanavalin A. (The intrinsic property of this protein is to bind sugar molecules; it is implicated in cell-to-cell recognition processes and is widely used as a laboratory diagnostic tool.) At CHESS we have used a 0.9 Å wavelength beam on station A1, fed by a 24 pole multipole wiggler. Both an imaging plate system and the Princeton 1k CCD detector [M. Tate et al., J. Appl. Cryst. 28, 196 (1995)] have been used on this experimental setup to collect diffraction data sets from frozen concanavalin A crystals (saccharide-free crystal form). The rapid readout of the CCD was most convenient compared with the image plate and its associated scanning and erasing. Moreover the data processing results towards the edges of the detectors, 0.98 Å, show that the CCD is much better than the image plate at recording these weaker data (Rmerge(I) 13% versus 44%, respectively). The poor performance of the image plate with weak signals has of course been documented by the Daresbury detector group [R. Lewis, J. Synchrotron Radiation 1, 43 (1994)]. However, the aperture of the CCD used was limiting here. Very recently, in another run at CHESS with the CCD on A1, we have been able to record diffraction data to 0.94 Å by further offsetting the detector. We again found that the reflections are still strong at the edge. Clearly the use of even shorter wavelengths than 0.9 Å would be very useful in matching the solid angle of the diffraction pattern to the available detector aperture, for a reasonable crystal-to-detector distance. In addition, absorption errors in the data can be simultaneously removed by such a strategy. Indeed, finely focused x-ray beams of, say 0.5 Å wavelength, are especially well suited to high energy, low emittance synchrotron radition (SR) machines. Some initial tests carried out on CHESS station F2 with a 0.5 Å wavelength beam and the CCD detector show an improvement in the R-merge(I) to 2 Å resolution, in comparison to the data collected at 0.9 Å wavelength (i.e., 2.3% versus 3.0%). In conclusion, the diffraction resolution limit (0.94 Å) seen already in our concanavalin A studies can be further enhanced and is important for the most detailed molecular model refinement (and the testing of structure solving strategies), in conjunction with novel spectroscopic and theoretical studies. This paper builds upon the work of Deacon et al. [Rev. Sci. Instrum. 66, 1287 (1995)].

Measured and calculated K-fluorescence effects on the MTF of an amorphous-selenium based CCD x-ray detector.

PubMed

Hunter, David M; Belev, George; Kasap, Safa; Yaffe, Martin J

2012-02-01

Theoretical reasoning suggests that direct conversion digital x-ray detectors based upon photoconductive amorphous-selenium (a-Se) could attain very high values of the MTF (modulation transfer function) at spatial frequencies well beyond 20 cycles mm(-1). One of the fundamental factors affecting resolution loss, particularly at x-ray energies just above the K-edge of selenium (12.66 keV), is the K-fluorescence reabsorption mechanism, wherein energy can be deposited in the detector at locations laterally displaced from the initial x-ray interaction site. This paper compares measured MTF changes above and below the Se K-edge of a CCD based a-Se x-ray detector with theoretical expectations. A prototype 25 μm sampling pitch (Nyquist frequency = 20 cycles mm(-1), 200 μm thick a-Se layer based x-ray detector, utilizing a specialized CCD readout device (200 × 400 area array), was used to make edge images with monochromatic x-rays above and below the K-edge of Se. A vacuum double crystal monochromator, exposed to polychromatic x-rays from a synchrotron, formed the monochromatic x-ray source. The monochromaticity of the x-rays was 99% or better. The presampling MTF was determined using the slanted edge method. The theory modeling the MTF performance of the detector includes the basic x-ray interaction physics in the a-Se layer as well as effects related to the operation of the CCD and charge trapping at a blocking layer present at the CCD/a-Se interface. The MTF performance of the prototype a-Se CCD was reduced from the theoretical value prescribed by the basic Se x-ray interaction physics, principally by the presence of a blocking layer. Nevertheless, the K-fluorescence reduction in the MTF was observed, approximately as predicted by theory. For the CCD prototype detector, at five cycles mm(-1), there was a 14% reduction of the MTF, from a value of 0.7 below the K-edge of Se, to 0.6 just above the K-edge. The MTF of an a-Se x-ray detector has been measured using monochromatic x-rays above and below the K-edge of selenium. The MTF is poorer above the K-edge by an amount consistent with theoretical expectations.

CCD filter and transform techniques for interference excision

NASA Technical Reports Server (NTRS)

Borsuk, G. M.; Dewitt, R. N.

1976-01-01

The theoretical and some experimental results of a study aimed at applying CCD filter and transform techniques to the problem of interference excision within communications channels were presented. Adaptive noise (interference) suppression was achieved by the modification of received signals such that they were orthogonal to the recently measured noise field. CCD techniques were examined to develop real-time noise excision processing. They were recursive filters, circulating filter banks, transversal filter banks, an optical implementation of the chirp Z transform, and a CCD analog FFT.

VizieR Online Data Catalog: BVR light curves of UZ Leo (Lee+, 2018)

NASA Astrophysics Data System (ADS)

Lee, J. W.; Park, J.-H.

2018-04-01

We performed new CCD photometry of UZ Leo during two observing seasons between 2012 February and 2013 April, using a PIXIS: 2048B CCD and a BVR filter set attached to the 61 cm reflector at Sobaeksan Optical Astronomy Observatory (SOAO) in Korea. The CCD chip has 2048x2048pixels and a pixel size of 13.5um, so the field of view of a CCD frame is 17.6'x17.6'. (1 data file).

Intense acoustic bursts as a signal-enhancement mechanism in ultrasound-modulated optical tomography.

PubMed

Kim, Chulhong; Zemp, Roger J; Wang, Lihong V

2006-08-15

Biophotonic imaging with ultrasound-modulated optical tomography (UOT) promises ultrasonically resolved imaging in biological tissues. A key challenge in this imaging technique is a low signal-to-noise ratio (SNR). We show significant UOT signal enhancement by using intense time-gated acoustic bursts. A CCD camera captured the speckle pattern from a laser-illuminated tissue phantom. Differences in speckle contrast were observed when ultrasonic bursts were applied, compared with when no ultrasound was applied. When CCD triggering was synchronized with burst initiation, acoustic-radiation-force-induced displacements were detected. To avoid mechanical contrast in UOT images, the CCD camera acquisition was delayed several milliseconds until transient effects of acoustic radiation force attenuated to a satisfactory level. The SNR of our system was sufficiently high to provide an image pixel per acoustic burst without signal averaging. Because of the substantially improved SNR, the use of intense acoustic bursts is a promising signal enhancement strategy for UOT.

Fundamental performance differences of CMOS and CCD imagers: part V

NASA Astrophysics Data System (ADS)

Janesick, James R.; Elliott, Tom; Andrews, James; Tower, John; Pinter, Jeff

2013-02-01

Previous papers delivered over the last decade have documented developmental progress made on large pixel scientific CMOS imagers that match or surpass CCD performance. New data and discussions presented in this paper include: 1) a new buried channel CCD fabricated on a CMOS process line, 2) new data products generated by high performance custom scientific CMOS 4T/5T/6T PPD pixel imagers, 3) ultimate CTE and speed limits for large pixel CMOS imagers, 4) fabrication and test results of a flight 4k x 4k CMOS imager for NRL's SoloHi Solar Orbiter Mission, 5) a progress report on ultra large stitched Mk x Nk CMOS imager, 6) data generated by on-chip sub-electron CDS signal chain circuitry used in our imagers, 7) CMOS and CMOSCCD proton and electron radiation damage data for dose levels up to 10 Mrd, 8) discussions and data for a new class of PMOS pixel CMOS imagers and 9) future CMOS development work planned.

A new spectroscopic imager for X-rays from 0.5 keV to 150 keV combining a pnCCD and a columnar CsI(Tl) scintillator

NASA Astrophysics Data System (ADS)

Schlosser, D. M.; Hartmann, R.; Kalok, D.; Bechteler, A.; Abboud, A.; Shokr, M.; Çonka, T.; Pietsch, U.; Strüder, L.

2017-04-01

By combining a low noise fully depleted pnCCD detector with a columnar CsI(Tl) scintillator an energy dispersive spatial resolving detector can be realized with a high quantum efficiency in the range from below 0.5 keV to above 150 keV. The used scintillator system increases the pulse height of gamma-rays converted in the CsI(Tl), due to focusing properties of the columnar scintillator structure by reducing the event size in indirect detection mode (conversion in the scintillator). In case of direct detection (conversion in the silicon of the pnCCD) the relative energy resolution is 0.7% at 122 keV (FWHM = 850 eV) and the spatial resolution is less than 75 μm. In case of indirect detection the relative energy resolution, integrated over all event sizes is about 9% at 122 keV with an expected spatial precision of below 75 μm.

CCD Camera Detection of HIV Infection.

PubMed

Day, John R

2017-01-01

Rapid and precise quantification of the infectivity of HIV is important for molecular virologic studies, as well as for measuring the activities of antiviral drugs and neutralizing antibodies. An indicator cell line, a CCD camera, and image-analysis software are used to quantify HIV infectivity. The cells of the P4R5 line, which express the receptors for HIV infection as well as β-galactosidase under the control of the HIV-1 long terminal repeat, are infected with HIV and then incubated 2 days later with X-gal to stain the infected cells blue. Digital images of monolayers of the infected cells are captured using a high resolution CCD video camera and a macro video zoom lens. A software program is developed to process the images and to count the blue-stained foci of infection. The described method allows for the rapid quantification of the infected cells over a wide range of viral inocula with reproducibility, accuracy and at relatively low cost.

Study the performance of star sensor influenced by space radiation damage of image sensor

NASA Astrophysics Data System (ADS)

Feng, Jie; Li, Yudong; Wen, Lin; Guo, Qi; Zhang, Xingyao

2018-03-01

Star sensor is an essential component of spacecraft attitude control system. Spatial radiation can cause star sensor performance degradation, abnormal work, attitude measurement accuracy and reliability reduction. Many studies have already been dedicated to the radiation effect on Charge-Coupled Device(CCD) image sensor, but fewer studies focus on the radiation effect of star sensor. The innovation of this paper is to study the radiation effects from the device level to the system level. The influence of the degradation of CCD image sensor radiation sensitive parameters on the performance parameters of star sensor is studied in this paper. The correlation among the radiation effect of proton, the non-uniformity noise of CCD image sensor and the performance parameter of star sensor is analyzed. This paper establishes a foundation for the study of error prediction and correction technology of star sensor on-orbit attitude measurement, and provides some theoretical basis for the design of high performance star sensor.

Design and development of a fiber optic TDI CCD-based slot-scan digital mammography system

NASA Astrophysics Data System (ADS)

Toker, Emre; Piccaro, Michele F.

1993-12-01

We previously reported on the development, design, and clinical evaluation of a CCD-based, high performance, filmless imaging system for stereotactic needle biopsy procedures in mammography. The MammoVision system has a limited imaging area of 50 mm X 50 mm, since it is designed specifically for breast biopsy applications. We are currently developing a new filmless imaging system designed to cover the 18 cm X 24 cm imaging area required for screening and diagnostic mammography. The diagnostic mammography system is based on four 1100 X 330 pixel format, full-frame, scientific grade, front illuminated, MPP mode CCDs, with 24 micrometers X 24 micrometers square pixels Each CCD is coupled to an x-ray intensifying screen via a 1.7:1 fiber optic reducer. The detector assembly (180 mm long and 13.5 mm wide) is scanned across the patient's breast synchronously with the x-ray source, with the CCDs operated in time-delay integration (TDI) mode. The total scan time is 4.0 seconds.

Comparison of lens- and fiber-coupled CCD detectors for X-ray computed tomography

PubMed Central

Uesugi, K.; Hoshino, M.; Yagi, N.

2011-01-01

X-ray imaging detectors with an identical phosphor and a CCD chip but employing lens- and fiber-coupling between them have been compared. These are designed for X-ray imaging experiments, especially computed tomography, at the medium-length beamline at the SPring-8 synchrotron radiation facility. It was found that the transmittance of light to the CCD is about four times higher in the fiber-coupled detector. The uniformity of response in the lens-coupled detector has a global shading of up to 40%, while pixel-to-pixel variation owing to a chicken-wire pattern was dominant in the fiber-coupled detector. Apart from the higher transmittance, the fiber-coupled detector has a few characteristics that require attention when it is used for computed tomography, which are browning of the fiber, discontinuity in the image, image distortion, and dark spots in the chicken-wire pattern. Thus, it is most suitable for high-speed tomography of samples that tend to deform, for example biological and soft materials. PMID:21335908

Securing quality of camera-based biomedical optics

NASA Astrophysics Data System (ADS)

Guse, Frank; Kasper, Axel; Zinter, Bob

2009-02-01

As sophisticated optical imaging technologies move into clinical applications, manufacturers need to guarantee their products meet required performance criteria over long lifetimes and in very different environmental conditions. A consistent quality management marks critical components features derived from end-users requirements in a top-down approach. Careful risk analysis in the design phase defines the sample sizes for production tests, whereas first article inspection assures the reliability of the production processes. We demonstrate the application of these basic quality principles to camera-based biomedical optics for a variety of examples including molecular diagnostics, dental imaging, ophthalmology and digital radiography, covering a wide range of CCD/CMOS chip sizes and resolutions. Novel concepts in fluorescence detection and structured illumination are also highlighted.

The Pariser-Parr-Pople model for trans-polyenes. I. Ab initio and semiempirical study of the bond alternation in trans-butadiene

NASA Astrophysics Data System (ADS)

Förner, Wolfgang

1992-03-01

Ab initio investigations of the bond alternation in butadiene are presented. The atomic basis sets applied range from minimal to split valence plus polarization quality. With the latter one the Hartree-Fock limit for the bond alternation is reached. Correlation is considered on Møller-Plesset many-body perturbation theory of second order (MP2), linear coupled cluster doubles (L-CCD) and coupled cluster doubles (CCD) level. For the smaller basis sets it is shown that for the bond alternation π-π correlations are essential while the effects of σ-σ and σ-π correlations are, though large, nearly independent of bond alternation. On MP2 level the variation of σ-π correlation with bond alternation is surprisingly large. This is discussed as an artefact of MP2. Comparative Su-Schrieffer-Heeger (SSH) and Pariser-Parr-Pople (PPP) calculations show that these models in their usual parametrizations cannot reproduce the ab initio results.

Observing variable stars at the University of Athens Observatory

NASA Astrophysics Data System (ADS)

Gazeas, K.; Manimanis, V. N.; Niarchos, P. G.

In 1999 the University of Athens installed a 0.4-m Cassegrain telescope (CCT-16, by DFM Engineering) on the roof of the Department of Astrophysics, Astronomy and Mechanics, equipped with a ST-8 CCD camera and Bessel UBVRI filters. Although the telescope was built for educational purposes, we found it can be a perfect research instrument, as we can obtain fine quality light curves of bright variable stars, even from a place close to the city center. Light curves of the δ Scuti star V1162 Ori and of the sdB star PG 1336-018 are presented, showing the ability of a 40-cm telescope to detect negligible luminosity fluctuations of relatively bright variable stars. To date, we succeed in making photometry of stars down to 15th magnitude with satisfactory results. We expect to achieve even better results in the future, as our methods still improve, and as the large number of relatively bright stars gives us the chance to study various fields of CCD photometry of variables.

Integrative Multi-Spectral Sensor Device for Far-Infrared and Visible Light Fusion

NASA Astrophysics Data System (ADS)

Qiao, Tiezhu; Chen, Lulu; Pang, Yusong; Yan, Gaowei

2018-06-01

Infrared and visible light image fusion technology is a hot spot in the research of multi-sensor fusion technology in recent years. Existing infrared and visible light fusion technologies need to register before fusion because of using two cameras. However, the application effect of the registration technology has yet to be improved. Hence, a novel integrative multi-spectral sensor device is proposed for infrared and visible light fusion, and by using the beam splitter prism, the coaxial light incident from the same lens is projected to the infrared charge coupled device (CCD) and visible light CCD, respectively. In this paper, the imaging mechanism of the proposed sensor device is studied with the process of the signals acquisition and fusion. The simulation experiment, which involves the entire process of the optic system, signal acquisition, and signal fusion, is constructed based on imaging effect model. Additionally, the quality evaluation index is adopted to analyze the simulation result. The experimental results demonstrate that the proposed sensor device is effective and feasible.

VizieR Online Data Catalog: Hα images of HD93521 (Rauw+, 2012)

NASA Astrophysics Data System (ADS)

Rauw, G.; Morel, T.; Palate, M.

2012-09-01

The best quality H-alpha images of the field around HD93521 are provided. These data were obtained by amateur astronomer Gaston Dessy (member of the Society Astronomique de Liege, Belgium) with a TMB-92 9.2cm refractor. The first image (haskyco1.fit) was taken with an Atik 16IC CCD and is the combination of 15 individual 5min exposures. The field of view covers 41x30.8-arcmin squared. HD93521 and BD+38 2183 are located on the pixels (328, 230) and (218, 390) respectively. A flat sky background was subtracted. The second image (haskyco2.fit) was taken with an Atik 4000M CCD and is the combination of 12 individual 5min exposures. The field of view covers 127.7x127.7arcmin squared. HD93521 and BD+38 2183 are located on the pixels (1109, 1545) and (989, 1385) respectively. A flat sky background was subtracted. (2 data files).

Occultation Predictions Using CCD Strip-Scanning Astrometry

NASA Technical Reports Server (NTRS)

Dunham, Edward W.; Ford, C. H.; Stone, R. P. S.; McDonald, S. W.; Olkin, C. B.; Elliot, J. L.; Witteborn, Fred C. (Technical Monitor)

1994-01-01

We are developing the method of CCD strip-scanning astrometry for the purpose of deriving reliable advance predictions for occultations involving small objects in the outer solar system. We are using a camera system based on a Ford/Loral 2Kx2K CCD with the Crossley telescope at Lick Observatory for this work. The columns of die CCD are aligned East-West, the telescope drive is stopped, and the CCD is clocked at the same rate that the stars drift across it. In this way we obtain arbitrary length strip images 20 arcmin wide with 0.58" pixels. Since planets move mainly in RA, it is possible to obtain images of the planet and star to be occulted on the same strip well before the occultation occurs. The strip-to-strip precision (i.e. reproducibility) of positions is limited by atmospheric image motion to about 0.1" rms per strip. However, for objects that are nearby in R.A., the image motion is highly correlated and their relative positions are good to 0.02" rms per strip. We will show that the effects of atmospheric image motion on a given strip can be removed if a sufficient number of strips of a given area have been obtained. Thus, it is possible to reach an rms precision of 0.02" per strip, corresponding to about 0.3 of Pluto or Triton's angular radius. The ultimate accuracy of a prediction based on strip-scanning astrometry is currently limited by the accuracy of the positions of the stars in the astrometric network used and by systematic errors most likely due to the optical system. We will show the results of . the prediction of some recent occultations as examples of the current capabilities and limitations of this technique.

Actinide chemistry using singlet-paired coupled cluster and its combinations with density functionals

NASA Astrophysics Data System (ADS)

Garza, Alejandro J.; Sousa Alencar, Ana G.; Scuseria, Gustavo E.

2015-12-01

Singlet-paired coupled cluster doubles (CCD0) is a simplification of CCD that relinquishes a fraction of dynamic correlation in order to be able to describe static correlation. Combinations of CCD0 with density functionals that recover specifically the dynamic correlation missing in the former have also been developed recently. Here, we assess the accuracy of CCD0 and CCD0+DFT (and variants of these using Brueckner orbitals) as compared to well-established quantum chemical methods for describing ground-state properties of singlet actinide molecules. The f0 actinyl series (UO22+, NpO23+, PuO24+), the isoelectronic NUN, and thorium (ThO, ThO2+) and nobelium (NoO, NoO2) oxides are studied.

Improving Kepler Pipeline Sensitivity with Pixel Response Function Photometry.

NASA Astrophysics Data System (ADS)

Morris, Robert L.; Bryson, Steve; Jenkins, Jon Michael; Smith, Jeffrey C

2014-06-01

We present the results of our investigation into the feasibility and expected benefits of implementing PRF-fitting photometry in the Kepler Science Processing Pipeline. The Kepler Pixel Response Function (PRF) describes the expected system response to a point source at infinity and includes the effects of the optical point spread function, the CCD detector responsivity function, and spacecraft pointing jitter. Planet detection in the Kepler pipeline is currently based on simple aperture photometry (SAP), which is most effective when applied to uncrowded bright stars. Its effectiveness diminishes rapidly as target brightness decreases relative to the effects of noise sources such as detector electronics, background stars, and image motion. In contrast, PRF photometry is based on fitting an explicit model of image formation to the data and naturally accounts for image motion and contributions of background stars. The key to obtaining high-quality photometry from PRF fitting is a high-quality model of the system's PRF, while the key to efficiently processing the large number of Kepler targets is an accurate catalog and accurate mapping of celestial coordinates onto the focal plane. If the CCD coordinates of stellar centroids are known a priori then the problem of PRF fitting becomes linear. A model of the Kepler PRF was constructed at the time of spacecraft commissioning by fitting piecewise polynomial surfaces to data from dithered full frame images. While this model accurately captured the initial state of the system, the PRF has evolved dynamically since then and has been seen to deviate significantly from the initial (static) model. We construct a dynamic PRF model which is then used to recover photometry for all targets of interest. Both simulation tests and results from Kepler flight data demonstrate the effectiveness of our approach. Kepler was selected as the 10th mission of the Discovery Program. Funding for this mission is provided by NASA’s Science Mission Directorate.Kepler was selected as the 10th mission of the Discovery Program. Funding for this mission is provided by NASA’s Science Mission Directorate.

The validated hypoallergenic cosmetics rating system: its 30-year evolution and effect on the prevalence of cosmetic reactions.

PubMed

Verallo-Rowell, Vermén M

2011-01-01

The validated hypoallergenic (vh) rating system was initiated in 1988 to try to objectively validate the "hypoallergenic" claim in cosmetics. To show how the system rates cosmetic hypoallergenicity and to compare the prevalence of cosmetic contact dermatitis (CCD) among users of regular cosmetics versus cosmetics with high VH numbers. (1) Made a VH list based on top allergens from patch-test results published by the North American Contact Dermatitis Group (NACDG) and the European Surveillance System on Contact Allergies (ESSCA); (2) reviewed global regulatory, cosmetic, drug, packaging, and manufacturing practices to show how allergens may contaminate products; (3) compared cosmetic ingredients lists against the VH list to obtain the VH rating (the more allergens absent, the higher the VH rating); and (4) obtained CCD prevalence among users of regular cosmetics versus users of cosmetics with high VH ratings. (1) Two VH lists (1988, 2003) included only cosmetic allergens in the NACDG surveys, the third (2007) included cosmetic and potential contaminant noncosmetic allergens, and the fourth (2010) adds ESSCA patch-test surveys. (2) CCD prevalence is 0.05 to 0.12% (average, 0.08%) among users of cosmetics with high VH ratings versus 2.4 to 36.3% among users of regular cosmetics. The VH rating system is shown to objectively validate the hypoallergenic cosmetics claim.

A programmable CCD driver circuit for multiphase CCD operation

NASA Technical Reports Server (NTRS)

Ewin, Audrey J.; Reed, Kenneth V.

1989-01-01

A programmable CCD (charge-coupled device) driver circuit was designed to drive CCDs in multiphased modes. The purpose of the drive electronics is to operate developmental CCD imaging arrays for NASA's tiltable moderate resolution imaging spectrometer (MODIS-T). Five objectives for the driver were considered during its design: (1) the circuit drives CCD electrode voltages between 0 V and +30 V to produce reasonable potential wells, (2) the driving sequence is started with one input signal, (3) the driving sequence is started with one input signal, (4) the circuit allows programming of frame sequences required by arrays of any size, (5) it produces interfacing signals for the CCD and the DTF (detector test facility). Simulation of the driver verified its function with the master clock running up to 10 MHz. This suggests a maximum rate of 400,000 pixels/s. Timing and packaging parameters were verified. The design uses 54 TTL (transistor-transistor logic) chips. Two versions of hardware were fabricated: wirewrap and printed circuit board. Both were verified functionally with a logic analyzer.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Su, Minfei; Li, Yue; Wyborny, Shane

ATG14 binding to BECN/Beclin homologs is essential for autophagy, a critical catabolic homeostasis pathway. Here, we show that the α-helical, coiled-coil domain (CCD) of BECN2, a recently identified mammalian BECN1 paralog, forms an antiparallel, curved homodimer with seven pairs of nonideal packing interactions, while the BECN2 CCD and ATG14 CCD form a parallel, curved heterodimer stabilized by multiple, conserved polar interactions. Compared to BECN1, the BECN2 CCD forms a weaker homodimer, but binds more tightly to the ATG14 CCD. Mutation of nonideal BECN2 interface residues to more ideal pairs improves homodimer self-association and thermal stability. Unlike BECN1, all BECN2 CCDmore » mutants bind ATG14, although more weakly than wild type. Thus, polar BECN2 CCD interface residues result in a metastable homodimer, facilitating dissociation, but enable better interactions with polar ATG14 residues stabilizing the BECN2:ATG14 heterodimer. These structure-based mechanistic differences in BECN1 and BECN2 homodimerization and heterodimerization likely dictate competitive ATG14 recruitment.« less

Enhanced mannitol biosynthesis by the fruit origin strain Fructobacillus tropaeoli CRL 2034.

PubMed

Ruiz Rodríguez, Luciana G; Aller, Kadri; Bru, Elena; De Vuyst, Luc; Hébert, Elvira M; Mozzi, Fernanda

2017-08-01

Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central composite design (CCD) as optimization strategy. The effect of the total saccharide (glucose + fructose, 1:2) content (TSC) in the medium (75, 100, 150, 200, and 225 g/l) and stirring (S; 50, 100, 200, 300 and 350 rpm) on mannitol production and yield by this strain was evaluated by using a 2 2 full-factorial CCD with 4 axial points (α = 1.5) and four replications of the center point, leading to 12 random experimental runs. Fermentations were carried out at 30 °C and pH 5.0 for 24 h. Minitab-15 software was used for experimental design and data analyses. The multiple response prediction analysis established 165 g/l of TSC and 200 rpm of S as optimal culture conditions to reach 85.03 g/l [95% CI (78.68, 91.39)] of mannitol and a yield of 82.02% [95% CI (71.98, 92.06)]. Finally, a validation experiment was conducted at the predicted optimum levels. The results obtained were 81.91 g/l of mannitol with a yield of 77.47% in outstanding agreement with the expected values. The mannitol 2-dehydrogenase enzyme activity was determined with 4.6-4.9 U/mg as the highest value found. To conclude, F. tropaeoli CRL 2034 produced high amounts of high-quality mannitol from fructose, being an excellent candidate for this polyol production.

A common origin for the bacterial toxin-antitoxin systems parD and ccd, suggested by analyses of toxin/target and toxin/antitoxin interactions.

PubMed

Smith, Andrew B; López-Villarejo, Juan; Diago-Navarro, Elizabeth; Mitchenall, Lesley A; Barendregt, Arjan; Heck, Albert J; Lemonnier, Marc; Maxwell, Anthony; Díaz-Orejas, Ramón

2012-01-01

Bacterial toxin-antitoxin (TA) systems encode two proteins, a potent inhibitor of cell proliferation (toxin) and its specific antidote (antitoxin). Structural data has revealed striking similarities between the two model TA toxins CcdB, a DNA gyrase inhibitor encoded by the ccd system of plasmid F, and Kid, a site-specific endoribonuclease encoded by the parD system of plasmid R1. While a common structural fold seemed at odds with the two clearly different modes of action of these toxins, the possibility of functional crosstalk between the parD and ccd systems, which would further point to their common evolutionary origin, has not been documented. Here, we show that the cleavage of RNA and the inhibition of protein synthesis by the Kid toxin, two activities that are specifically counteracted by its cognate Kis antitoxin, are altered, but not inhibited, by the CcdA antitoxin. In addition, Kis was able to inhibit the stimulation of DNA gyrase-mediated cleavage of DNA by CcdB, albeit less efficiently than CcdA. We further show that physical interactions between the toxins and antitoxins of the different systems do occur and define the stoichiometry of the complexes formed. We found that CcdB did not degrade RNA nor did Kid have any reproducible effect on the tested DNA gyrase activities, suggesting that these toxins evolved to reach different, rather than common, cellular targets.

Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera).

PubMed

Johnson, Reed M; Evans, Jay D; Robinson, Gene E; Berenbaum, May R

2009-09-01

Colony collapse disorder (CCD) is a mysterious disappearance of honey bees that has beset beekeepers in the United States since late 2006. Pathogens and other environmental stresses, including pesticides, have been linked to CCD, but a causal relationship has not yet been demonstrated. Because the gut acts as a primary interface between the honey bee and its environment as a site of entry for pathogens and toxins, we used whole-genome microarrays to compare gene expression between guts of bees from CCD colonies originating on both the east and west coasts of the United States and guts of bees from healthy colonies sampled before the emergence of CCD. Considerable variation in gene expression was associated with the geographical origin of bees, but a consensus list of 65 transcripts was identified as potential markers for CCD status. Overall, elevated expression of pesticide response genes was not observed. Genes involved in immune response showed no clear trend in expression pattern despite the increased prevalence of viruses and other pathogens in CCD colonies. Microarray analysis revealed unusual ribosomal RNA fragments that were conspicuously more abundant in the guts of CCD bees. The presence of these fragments may be a possible consequence of picorna-like viral infection, including deformed wing virus and Israeli acute paralysis virus, and may be related to arrested translation. Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD.

Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)

PubMed Central

Johnson, Reed M.; Evans, Jay D.; Robinson, Gene E.; Berenbaum, May R.

2009-01-01

Colony collapse disorder (CCD) is a mysterious disappearance of honey bees that has beset beekeepers in the United States since late 2006. Pathogens and other environmental stresses, including pesticides, have been linked to CCD, but a causal relationship has not yet been demonstrated. Because the gut acts as a primary interface between the honey bee and its environment as a site of entry for pathogens and toxins, we used whole-genome microarrays to compare gene expression between guts of bees from CCD colonies originating on both the east and west coasts of the United States and guts of bees from healthy colonies sampled before the emergence of CCD. Considerable variation in gene expression was associated with the geographical origin of bees, but a consensus list of 65 transcripts was identified as potential markers for CCD status. Overall, elevated expression of pesticide response genes was not observed. Genes involved in immune response showed no clear trend in expression pattern despite the increased prevalence of viruses and other pathogens in CCD colonies. Microarray analysis revealed unusual ribosomal RNA fragments that were conspicuously more abundant in the guts of CCD bees. The presence of these fragments may be a possible consequence of picorna-like viral infection, including deformed wing virus and Israeli acute paralysis virus, and may be related to arrested translation. Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD. PMID:19706391

Cognitive-communication disorder following right hemisphere stroke: exploring rehabilitation access and outcomes.

PubMed

Hewetson, Ronelle; Cornwell, Petrea; Shum, David

2017-07-01

Rehabilitation positively influences return to activities and social roles in people with aphasia. The cognitive-communication disorder (CCD) found following a right hemisphere stroke has been less extensively researched with rehabilitation access and outcomes yet to be determined. To document rehabilitation access and outcomes for people with CCD post-stroke; and compare outcomes based on presence (viz CCD; aphasia) or absence of communication impairment. A retrospective chart audit was completed for patients with first onset unilateral stroke, with a hospital length of stay (LOS) of at least two days and a communication assessment by a speech pathologist. Data extracted included presence and severity of communication impairment, access to and LOS in a rehabilitation unit, and functional outcome measures recorded at rehabilitation discharge. The majority of the 115 patients who met inclusion criteria were living independently (n = 112, 97.4%) at the time of stroke. CCD (66%) was diagnosed with similar frequency to aphasia (68%). The presence of communication impairment did not result in significant differences in rehabilitation LOS and discharge destination when compared to hemispheric strokes without communication impairment. Severity of CCD was an independent predictor of functional gain by rehabilitation discharge. People with CCD require comparable access to rehabilitation as people with aphasia, and severity of CCD should be considered in determining rehabilitation LOS. A large number of people are discharged with ongoing CCD which warrants exploration of potential participation restrictions created by the communication impairment.

A Common Origin for the Bacterial Toxin-Antitoxin Systems parD and ccd, Suggested by Analyses of Toxin/Target and Toxin/Antitoxin Interactions

PubMed Central

Mitchenall, Lesley A.; Barendregt, Arjan; Heck, Albert J.; Lemonnier, Marc; Maxwell, Anthony; Díaz-Orejas, Ramón

2012-01-01

Bacterial toxin-antitoxin (TA) systems encode two proteins, a potent inhibitor of cell proliferation (toxin) and its specific antidote (antitoxin). Structural data has revealed striking similarities between the two model TA toxins CcdB, a DNA gyrase inhibitor encoded by the ccd system of plasmid F, and Kid, a site-specific endoribonuclease encoded by the parD system of plasmid R1. While a common structural fold seemed at odds with the two clearly different modes of action of these toxins, the possibility of functional crosstalk between the parD and ccd systems, which would further point to their common evolutionary origin, has not been documented. Here, we show that the cleavage of RNA and the inhibition of protein synthesis by the Kid toxin, two activities that are specifically counteracted by its cognate Kis antitoxin, are altered, but not inhibited, by the CcdA antitoxin. In addition, Kis was able to inhibit the stimulation of DNA gyrase-mediated cleavage of DNA by CcdB, albeit less efficiently than CcdA. We further show that physical interactions between the toxins and antitoxins of the different systems do occur and define the stoichiometry of the complexes formed. We found that CcdB did not degrade RNA nor did Kid have any reproducible effect on the tested DNA gyrase activities, suggesting that these toxins evolved to reach different, rather than common, cellular targets. PMID:23029540

Visualization of permanent marks in progressive addition lenses by digital in-line holography

NASA Astrophysics Data System (ADS)

Perucho, Beatriz; Micó, Vicente

2013-04-01

A critical issue in the production of ophthalmic lenses is to guarantee the correct centering and alignment throughout the manufacturing and mounting processes. Aimed to that, progressive addition lenses (PALs) incorporate permanent marks at standardized locations at the lens. Those marks are engraved upon the surface and provide the model identification and addition power of the PAL, as well as to serve as locator marks to re-ink the removable marks again if necessary. Although the permanent marks should be visible by simple visual inspection, those marks are often faint and weak on new lenses providing low contrast, obscured by scratches on older lenses, and partially occluded and difficult to recognize on tinted or anti-reflection coated lenses. In this contribution, we present an extremely simple visualization system for permanent marks in PALs based on digital in-line holography. Light emitted by a superluminescent diode (SLD) is used to illuminate the PAL which is placed just before a digital (CCD) sensor. Thus, the CCD records an in-line hologram incoming from the diffracted wavefront provided by the PAL. As a result, it is possible to recover an in-focus image of the PAL inspected region by means of classical holographic tools applied in the digital domain. This numerical process involves digital recording of the in-line hologram, numerical back propagation to the PAL plane, and some digital processing to reduce noise and present a high quality final image. Preliminary experimental results are provided showing the applicability of the proposed method.

A complex noise reduction method for improving visualization of SD-OCT skin biomedical images

NASA Astrophysics Data System (ADS)

Myakinin, Oleg O.; Zakharov, Valery P.; Bratchenko, Ivan A.; Kornilin, Dmitry V.; Khramov, Alexander G.

2014-05-01

In this paper we consider the original method of solving noise reduction problem for visualization's quality improvement of SD-OCT skin and tumors biomedical images. The principal advantages of OCT are high resolution and possibility of in vivo analysis. We propose a two-stage algorithm: 1) process of raw one-dimensional A-scans of SD-OCT and 2) remove a noise from the resulting B(C)-scans. The general mathematical methods of SD-OCT are unstable: if the noise of the CCD is 1.6% of the dynamic range then result distortions are already 25-40% of the dynamic range. We use at the first stage a resampling of A-scans and simple linear filters to reduce the amount of data and remove the noise of the CCD camera. The efficiency, improving productivity and conservation of the axial resolution when using this approach are showed. At the second stage we use an effective algorithms based on Hilbert-Huang Transform for more accurately noise peaks removal. The effectiveness of the proposed approach for visualization of malignant and benign skin tumors (melanoma, BCC etc.) and a significant improvement of SNR level for different methods of noise reduction are showed. Also in this study we consider a modification of this method depending of a specific hardware and software features of used OCT setup. The basic version does not require any hardware modifications of existing equipment. The effectiveness of proposed method for 3D visualization of tissues can simplify medical diagnosis in oncology.

Small angle light scattering characterization of single micrometric particles in microfluidic flows

NASA Astrophysics Data System (ADS)

Dannhauser, David; Romeo, Giovanni; Causa, Filippo; Netti, Paolo A.

2013-04-01

A CCD-camera based small angle light scattering (SALS) apparatus has been used to characterize single micrometric particles flowing in a micro-channel. The measured scattering vector spans the range 2x10-2 - 6:8x101μm-1. The incident laser light is collimated to a spot of about 50 μm in diameter at the sample position with a divergence lower than 0.045 rad. Such small collimated laser beam opens the possibility to perform on-line SALS of micron-sized particles flowing in micro-channels. By properly designing the micro-channel and using a viscoelastic liquid as suspending medium we are able to realize a precise 3D focusing of the target particles. The forward scattering emitted from the particle is collected by a lens with high numerical aperture. At the focal point of that lens a homemade beam stop is blocking the incident light. Finally, a second lens maps the scattered light on the CCD sensor, allowing to obtain far field images on short distances. Measurements with mono-disperse polystyrene particles, both in quiescent and in-flow conditions have been realized. Experiments in-flow allow to measure the single particle scattering. Results are validated by comparison with calculations based on the Lorenz-Mie theory. The quality of the measured intensity profiles confirms the possibility to use our apparatus in real multiplex applications, with particles down to 1 μm in radius.

CHIP Regulates Aquaporin-2 Quality Control and Body Water Homeostasis.

PubMed

Wu, Qi; Moeller, Hanne B; Stevens, Donté A; Sanchez-Hodge, Rebekah; Childers, Gabrielle; Kortenoeven, Marleen L A; Cheng, Lei; Rosenbaek, Lena L; Rubel, Carrie; Patterson, Cam; Pisitkun, Trairak; Schisler, Jonathan C; Fenton, Robert A

2018-03-01

The importance of the kidney distal convoluted tubule (DCT) and cortical collecting duct (CCD) is highlighted by various water and electrolyte disorders that arise when the unique transport properties of these segments are disturbed. Despite this critical role, little is known about which proteins have a regulatory role in these cells and how these cells can be regulated by individual physiologic stimuli. By combining proteomics, bioinformatics, and cell biology approaches, we found that the E3 ubiquitin ligase CHIP is highly expressed throughout the collecting duct; is modulated in abundance by vasopressin; interacts with aquaporin-2 (AQP2), Hsp70, and Hsc70; and can directly ubiquitylate the water channel AQP2 in vitro shRNA knockdown of CHIP in CCD cells increased AQP2 protein t 1/2 and reduced AQP2 ubiquitylation, resulting in greater levels of AQP2 and phosphorylated AQP2. CHIP knockdown increased the plasma membrane abundance of AQP2 in these cells. Compared with wild-type controls, CHIP knockout mice or novel CRISPR/Cas9 mice without CHIP E3 ligase activity had greater AQP2 abundance and altered renal water handling, with decreased water intake and urine volume, alongside higher urine osmolality. We did not observe significant changes in other water- or sodium-transporting proteins in the gene-modified mice. In summary, these results suggest that CHIP regulates AQP2 and subsequently, renal water handling. Copyright © 2018 by the American Society of Nephrology.

A Compact Imaging Detector of Polarization and Spectral Content

NASA Technical Reports Server (NTRS)

Rust, D. M.; Kumar, A.; Thompson, K. E.

1993-01-01

A new type of image detector will simultaneously analyze the polarization of light at all picture elements in a scene. The integrated Dual Imaging Detector (IDID) consists of a polarizing beam splitter bonded to a charge-coupled device (CCD), with signal-analysis circuitry and analog-to-digital converters, all integrated on a silicon chip. The polarizing beam splitter can be either a Ronchi ruling, or an array of cylindrical lenslets, bonded to a birefringent wafer. The wafer, in turn, is bonded to the CCD so that light in the two orthogonal planes of polarization falls on adjacent pairs of pixels. The use of a high-index birefringent material, e.g., rutile, allows the IDID to operate at f-numbers as high as f/3.5. Other aspects of the detector are discussed.

Time and space integrating acousto-optic folded spectrum processing for SETI

NASA Technical Reports Server (NTRS)

Wagner, K.; Psaltis, D.

1986-01-01

Time and space integrating folded spectrum techniques utilizing acousto-optic devices (AOD) as 1-D input transducers are investigated for a potential application as wideband, high resolution, large processing gain spectrum analyzers in the search for extra-terrestrial intelligence (SETI) program. The space integrating Fourier transform performed by a lens channels the coarse spectral components diffracted from an AOD onto an array of time integrating narrowband fine resolution spectrum analyzers. The pulsing action of a laser diode samples the interferometrically detected output, aliasing the fine resolution components to baseband, as required for the subsequent charge coupled devices (CCD) processing. The raster scan mechanism incorporated into the readout of the CCD detector array is used to unfold the 2-D transform, reproducing the desired high resolution Fourier transform of the input signal.

Variable high-resolution color CCD camera system with online capability for professional photo studio application

NASA Astrophysics Data System (ADS)

Breitfelder, Stefan; Reichel, Frank R.; Gaertner, Ernst; Hacker, Erich J.; Cappellaro, Markus; Rudolf, Peter; Voelk, Ute

1998-04-01

Digital cameras are of increasing significance for professional applications in photo studios where fashion, portrait, product and catalog photographs or advertising photos of high quality have to be taken. The eyelike is a digital camera system which has been developed for such applications. It is capable of working online with high frame rates and images of full sensor size and it provides a resolution that can be varied between 2048 by 2048 and 6144 by 6144 pixel at a RGB color depth of 12 Bit per channel with an also variable exposure time of 1/60s to 1s. With an exposure time of 100 ms digitization takes approx. 2 seconds for an image of 2048 by 2048 pixels (12 Mbyte), 8 seconds for the image of 4096 by 4096 pixels (48 Mbyte) and 40 seconds for the image of 6144 by 6144 pixels (108 MByte). The eyelike can be used in various configurations. Used as a camera body most commercial lenses can be connected to the camera via existing lens adaptors. On the other hand the eyelike can be used as a back to most commercial 4' by 5' view cameras. This paper describes the eyelike camera concept with the essential system components. The article finishes with a description of the software, which is needed to bring the high quality of the camera to the user.

The Operation and Evolution of the Swift X-ray Telescope

NASA Technical Reports Server (NTRS)

Kennea, Jamie; Burrows, D. N.; Pagani, C.; Hill, Joanne; Racusin, J. L.; Morris, D. C.; Abbey, A. F.; Beardmore, A. P.; Campana, G.; Chincarini, G.;

Optical readout of a two phase liquid argon TPC using CCD camera and THGEMs

NASA Astrophysics Data System (ADS)

Mavrokoridis, K.; Ball, F.; Carroll, J.; Lazos, M.; McCormick, K. J.; Smith, N. A.; Touramanis, C.; Walker, J.

2014-02-01

This paper presents a preliminary study into the use of CCDs to image secondary scintillation light generated by THick Gas Electron Multipliers (THGEMs) in a two phase LAr TPC. A Sony ICX285AL CCD chip was mounted above a double THGEM in the gas phase of a 40 litre two-phase LAr TPC with the majority of the camera electronics positioned externally via a feedthrough. An Am-241 source was mounted on a rotatable motion feedthrough allowing the positioning of the alpha source either inside or outside of the field cage. Developed for and incorporated into the TPC design was a novel high voltage feedthrough featuring LAr insulation. Furthermore, a range of webcams were tested for operation in cryogenics as an internal detector monitoring tool. Of the range of webcams tested the Microsoft HD-3000 (model no:1456) webcam was found to be superior in terms of noise and lowest operating temperature. In ambient temperature and atmospheric pressure 1 ppm pure argon gas, the THGEM gain was ≈ 1000 and using a 1 msec exposure the CCD captured single alpha tracks. Successful operation of the CCD camera in two-phase cryogenic mode was also achieved. Using a 10 sec exposure a photograph of secondary scintillation light induced by the Am-241 source in LAr has been captured for the first time.

CCD Detects Two Images In Quick Succession

NASA Technical Reports Server (NTRS)

Janesick, James R.; Collins, Andy

1996-01-01

Prototype special-purpose charge-coupled device (CCD) designed to detect two 1,024 x 1,024-pixel images in rapid succession. Readout performed slowly to minimize noise. CCD operated in synchronism with pulsed laser, stroboscope, or other pulsed source of light to form pairs of images of rapidly moving objects.

IAPV, a bee-affecting virus associated with Colony Collapse Disorder can be silenced by dsRNA ingestion.

PubMed

Maori, E; Paldi, N; Shafir, S; Kalev, H; Tsur, E; Glick, E; Sela, I

2009-02-01

Colony Collapse Disorder (CCD) has been associated with Israeli acute paralysis virus (IAPV). CCD poses a serious threat to apiculture and agriculture as a whole, due to the consequent inability to provide the necessary amount of bees for pollination of critical crops. Here we report on RNAi-silencing of IAPV infection by feeding bees with double-stranded RNA, as an efficient and feasible way of controlling this viral disease. The association of CCD with IAPV is discussed, as well as the potential of controlling CCD.

SCP -- A Simple CCD Processing Package

NASA Astrophysics Data System (ADS)

Lewis, J. R.

This note describes a small set of programs, written at RGO, which deal with basic CCD frame processing (e.g. bias subtraction, flat fielding, trimming etc.). The need to process large numbers of CCD frames from devices such as FOS or ISIS in order to extract spectra has prompted the writing of routines which will do the basic hack-work with a minimal amount of interaction from the user. Although they were written with spectral data in mind, there are no ``spectrum-specific'' features in the software which means they can be applied to any CCD data.

Time-saving design of experiment protocol for optimization of LC-MS data processing in metabolomic approaches.

PubMed

Zheng, Hong; Clausen, Morten Rahr; Dalsgaard, Trine Kastrup; Mortensen, Grith; Bertram, Hanne Christine

2013-08-06

We describe a time-saving protocol for the processing of LC-MS-based metabolomics data by optimizing parameter settings in XCMS and threshold settings for removing noisy and low-intensity peaks using design of experiment (DoE) approaches including Plackett-Burman design (PBD) for screening and central composite design (CCD) for optimization. A reliability index, which is based on evaluation of the linear response to a dilution series, was used as a parameter for the assessment of data quality. After identifying the significant parameters in the XCMS software by PBD, CCD was applied to determine their values by maximizing the reliability and group indexes. Optimal settings by DoE resulted in improvements of 19.4% and 54.7% in the reliability index for a standard mixture and human urine, respectively, as compared with the default setting, and a total of 38 h was required to complete the optimization. Moreover, threshold settings were optimized by using CCD for further improvement. The approach combining optimal parameter setting and the threshold method improved the reliability index about 9.5 times for a standards mixture and 14.5 times for human urine data, which required a total of 41 h. Validation results also showed improvements in the reliability index of about 5-7 times even for urine samples from different subjects. It is concluded that the proposed methodology can be used as a time-saving approach for improving the processing of LC-MS-based metabolomics data.

Small-angle solution scattering using the mixed-mode pixel array detector.

PubMed

Koerner, Lucas J; Gillilan, Richard E; Green, Katherine S; Wang, Suntao; Gruner, Sol M

2011-03-01

Solution small-angle X-ray scattering (SAXS) measurements were obtained using a 128 × 128 pixel X-ray mixed-mode pixel array detector (MMPAD) with an 860 µs readout time. The MMPAD offers advantages for SAXS experiments: a pixel full-well of >2 × 10(7) 10 keV X-rays, a maximum flux rate of 10(8) X-rays pixel(-1) s(-1), and a sub-pixel point-spread function. Data from the MMPAD were quantitatively compared with data from a charge-coupled device (CCD) fiber-optically coupled to a phosphor screen. MMPAD solution SAXS data from lysozyme solutions were of equal or better quality than data captured by the CCD. The read-noise (normalized by pixel area) of the MMPAD was less than that of the CCD by an average factor of 3.0. Short sample-to-detector distances were required owing to the small MMPAD area (19.2 mm × 19.2 mm), and were revealed to be advantageous with respect to detector read-noise. As predicted by the Shannon sampling theory and confirmed by the acquisition of lysozyme solution SAXS curves, the MMPAD at short distances is capable of sufficiently sampling a solution SAXS curve for protein shape analysis. The readout speed of the MMPAD was demonstrated by continuously monitoring lysozyme sample evolution as radiation damage accumulated. These experiments prove that a small suitably configured MMPAD is appropriate for time-resolved solution scattering measurements.

Small-angle solution scattering using the mixed-mode pixel array detector

PubMed Central

Koerner, Lucas J.; Gillilan, Richard E.; Green, Katherine S.; Wang, Suntao; Gruner, Sol M.

2011-01-01

Solution small-angle X-ray scattering (SAXS) measurements were obtained using a 128 × 128 pixel X-ray mixed-mode pixel array detector (MMPAD) with an 860 µs readout time. The MMPAD offers advantages for SAXS experiments: a pixel full-well of >2 × 107 10 keV X-rays, a maximum flux rate of 108 X-rays pixel−1 s−1, and a sub-pixel point-spread function. Data from the MMPAD were quantitatively compared with data from a charge-coupled device (CCD) fiber-optically coupled to a phosphor screen. MMPAD solution SAXS data from lysozyme solutions were of equal or better quality than data captured by the CCD. The read-noise (normalized by pixel area) of the MMPAD was less than that of the CCD by an average factor of 3.0. Short sample-to-detector distances were required owing to the small MMPAD area (19.2 mm × 19.2 mm), and were revealed to be advantageous with respect to detector read-noise. As predicted by the Shannon sampling theory and confirmed by the acquisition of lysozyme solution SAXS curves, the MMPAD at short distances is capable of sufficiently sampling a solution SAXS curve for protein shape analysis. The readout speed of the MMPAD was demonstrated by continuously monitoring lysozyme sample evolution as radiation damage accumulated. These experiments prove that a small suitably configured MMPAD is appropriate for time-resolved solution scattering measurements. PMID:21335900

Proton radiation damage assessment of a CCD for use in a Ultraviolet and Visible Spectrometer

NASA Astrophysics Data System (ADS)

Gow, J. P. D.; Mason, J.; Leese, M.; Hathi, B.; Patel, M.

2017-01-01

This paper describes the radiation environment and radiation damage analysis performed for the Nadir and Occultation for MArs Discovery (NOMAD) Ultraviolet and Visible Spectrometer (UVIS) channel launched onboard the ExoMars Trace Gas Orbiter (TGO) in 2016. The aim of the instrument is to map the temporal and spatial variation of trace gases such as ozone and dust/cloud aerosols in the atmosphere of Mars. The instrument consists of a set of two miniature telescope viewing optics which allow for selective input onto the optical bench, where an e2v technologies CCD30-11 will be used as the detector. A Geometry Description Markup Language model of the spacecraft and instrument box was created and through the use of ESA's SPace ENVironment Information System (SPENVIS) an estimate of the 10 MeV equivalent proton fluence was made at a number of radiation sensitive regions within NOMAD, including that of the CCD30-11 which is the focus of this paper. The end of life 10 MeV equivalent proton fluence at the charge coupled device was estimated to be 4.7 × 109 protons.cm-2 three devices were irradiated at different levels up a 10 MeV equivalent fluence of 9.4 × 109 protons.cm-2. The dark current, charge transfer inefficiency, charge storage, and cosmetic quality of the devices was investigated pre- and post-irradiation, determining that the devices will continue to provide excellent science throughout the mission.

The astrometric lessons of Gaia-GBOT experiment

NASA Astrophysics Data System (ADS)

Bouquillon, S.; Mendez, R. A.; Altmann, M.

2017-07-01

To ensure the full capabilities of the Gaia's measurements, a programme of daily observations with Earth-based telescopes of the satellite itself - called Ground Based Optical Tracking (GBOT) - was implemented since the beginning of the Gaia mission (for more details concerning GBOT operating see Altmann et al. 2014 and concerning GBOT software facilities see Bouquillon et al. 2014). These observations are carried out mainly with two facilities: the 2.6m VLT Survey Telescope (ESO's VST) at the Cerro Paranal in Chile and the 2.0m Liverpool Telescope (LT) on the Canary Island of La Palma. The constraint of 20 mas on the tracking astrometric quality and the fact that Gaia is a faint and relatively fast moving target (its magnitude in a red passband is around 21 and its apparent speed around 0.04"/s), lead us to rigorously analyse the reachable astrometric precision for CCD observations of this kind of celestial objects. During LARIM 2016, we presented the main results of this study which uses the Cramér-Rao lower bound to characterize the precision limit for the PSF center when drifting in the CCD-frame. This work extends earlier studies dealing with one-dimensional detectors and stationary sources (Mendez et al. 2013 & 2014) firstly to the case of standard two-dimensional CCD sensors, and then, to moving sources. These new results have been submitted for a publication in A&A journal this year (Bouquillon et al. 2017).

Software design of control system of CCD side-scatter lidar

NASA Astrophysics Data System (ADS)

Kuang, Zhiqiang; Liu, Dong; Deng, Qian; Zhang, Zhanye; Wang, Zhenzhu; Yu, Siqi; Tao, Zongming; Xie, Chenbo; Wang, Yingjian

2018-03-01

Because of the existence of blind zone and transition zone, the application of backscattering lidar in near-ground is limited. The side-scatter lidar equipped with the Charge Coupled Devices (CCD) can separate the transmitting and receiving devices to avoid the impact of the geometric factors which is exited in the backscattering lidar and, detect the more precise near-ground aerosol signals continuously. Theories of CCD side-scatter lidar and the design of control system are introduced. The visible control of laser and CCD and automatic data processing method of the side-scatter lidar are developed by using the software of Visual C #. The results which are compared with the calibration of the atmospheric aerosol lidar data show that signals from the CCD side- scatter lidar are convincible.

Regulation of transport in the connecting tubule and cortical collecting duct

PubMed Central

Staruschenko, Alexander

2012-01-01

The central goal of this overview article is to summarize recent findings in renal epithelial transport, focusing chiefly on the connecting tubule (CNT) and the cortical collecting duct (CCD). Mammalian CCD and CNT are involved in fine tuning of electrolyte and fluid balance through reabsorption and secretion. Specific transporters and channels mediate vectorial movements of water and solutes in these segments. Although only a small percent of the glomerular filtrate reaches the CNT and CCD, these segments are critical for water and electrolyte homeostasis since several hormones, e.g. aldosterone and arginine vasopressin, exert their main effects in these nephron sites. Importantly, hormones regulate the function of the entire nephron and kidney by affecting channels and transporters in the CNT and CCD. Knowledge about the physiological and pathophysiological regulation of transport in the CNT and CCD and particular roles of specific channels/transporters has increased tremendously over the last two decades. Recent studies shed new light on several key questions concerning the regulation of renal transport. Precise distribution patterns of transport proteins in the CCD and CNT will be reviewed, and their physiological roles and mechanisms mediating ion transport in these segments will be also covered. Special emphasis will be given to pathophysiological conditions appearing as a result of abnormalities in renal transport in the CNT and CCD. PMID:23227301

Silicon sample holder for molecular beam epitaxy on pre-fabricated integrated circuits

NASA Technical Reports Server (NTRS)

Hoenk, Michael E. (Inventor); Grunthaner, Paula J. (Inventor); Grunthaner, Frank J. (Inventor)

1994-01-01

The sample holder of the invention is formed of the same semiconductor crystal as the integrated circuit on which the molecular beam expitaxial process is to be performed. In the preferred embodiment, the sample holder comprises three stacked micro-machined silicon wafers: a silicon base wafer having a square micro-machined center opening corresponding in size and shape to the active area of a CCD imager chip, a silicon center wafer micro-machined as an annulus having radially inwardly pointing fingers whose ends abut the edges of and center the CCD imager chip within the annulus, and a silicon top wafer micro-machined as an annulus having cantilevered membranes which extend over the top of the CCD imager chip. The micro-machined silicon wafers are stacked in the order given above with the CCD imager chip centered in the center wafer and sandwiched between the base and top wafers. The thickness of the center wafer is about 20% less than the thickness of the CCD imager chip. Preferably, four titanium wires, each grasping the edges of the top and base wafers, compress all three wafers together, flexing the cantilever fingers of the top wafer to accommodate the thickness of the CCD imager chip, acting as a spring holding the CCD imager chip in place.

Design and implementation of fast bipolar clock drivers for CCD imaging systems in space applications

NASA Astrophysics Data System (ADS)

Jayarajan, Jayesh; Kumar, Nishant; Verma, Amarnath; Thaker, Ramkrishna

2016-05-01

Drive electronics for generating fast, bipolar clocks, which can drive capacitive loads of the order of 5-10nF are indispensable for present day Charge Coupled Devices (CCDs). Design of these high speed bipolar clocks is challenging because of the capacitive loads that have to be driven and a strict constraint on the rise and fall times. Designing drive electronics circuits for space applications becomes even more challenging due to limited number of available discrete devices, which can survive in the harsh radiation prone space environment. This paper presents the design, simulations and test results of a set of such high speed, bipolar clock drivers. The design has been tested under a thermal cycle of -15 deg C to +55 deg C under vacuum conditions and has been designed using radiation hardened components. The test results show that the design meets the stringent rise/fall time requirements of 50+/-10ns for Multiple Vertical CCD (VCCD) clocks and 20+/-5ns for Horizontal CCD (HCCD) clocks with sufficient design margins across full temperature range, with a pixel readout rate of 6.6MHz. The full design has been realized in flexi-rigid PCB with package volume of 140x160x50 mm3.

A 5- μ m pitch charge-coupled device optimized for resonant inelastic soft X-ray scattering

DOE PAGES

Andresen, N. C.; Denes, P.; Goldschmidt, A.; ...

2017-08-08

Here, we have developed a charge-coupled device (CCD) with 5 μm × 45 μm pixels on high-resistivity silicon. The fully depleted 200 μm-thick silicon detector is back-illuminated through a 10 nm-thick in situ doped polysilicon window and is thus highly efficient for soft through > 8 keV hard X-rays. The device described here is a 1.5 megapixel CCD with 2496 × 620 pixels. The pixel and camera geometry was optimized for Resonant Inelastic X-ray Scattering (RIXS) and is particularly advantageous for spectrometers with limited arm lengths. In this article, we describe the device architecture, construction and operation, and its performancemore » during tests at the Advance Light Source (ALS) 8.0.1 RIXS beamline. The improved spectroscopic performance, when compared with a current standard commercial camera, is demonstrated with a ~280 eV (C K) X-ray beam on a graphite sample. Readout noise is typically 3-6 electrons and the point spread function for soft C K X-rays in the 5 μm direction is 4.0 μm ± 0.2 μm. Finally, the measured quantum efficiency of the CCD is greater than 75% in the range from 200 eV to 1 keV.« less

A 5-μm pitch charge-coupled device optimized for resonant inelastic soft X-ray scattering

NASA Astrophysics Data System (ADS)

Andresen, N. C.; Denes, P.; Goldschmidt, A.; Joseph, J.; Karcher, A.; Tindall, C. S.

2017-08-01

We have developed a charge-coupled device (CCD) with 5 μm × 45 μm pixels on high-resistivity silicon. The fully depleted 200 μm-thick silicon detector is back-illuminated through a 10 nm-thick in situ doped polysilicon window and is thus highly efficient for soft through >8 keV hard X-rays. The device described here is a 1.5 megapixel CCD with 2496 × 620 pixels. The pixel and camera geometry was optimized for Resonant Inelastic X-ray Scattering (RIXS) and is particularly advantageous for spectrometers with limited arm lengths. In this article, we describe the device architecture, construction and operation, and its performance during tests at the Advance Light Source (ALS) 8.0.1 RIXS beamline. The improved spectroscopic performance, when compared with a current standard commercial camera, is demonstrated with a ˜280 eV (CK) X-ray beam on a graphite sample. Readout noise is typically 3-6 electrons and the point spread function for soft CK X-rays in the 5 μm direction is 4.0 μm ± 0.2 μm. The measured quantum efficiency of the CCD is greater than 75% in the range from 200 eV to 1 keV.

A 5-μm pitch charge-coupled device optimized for resonant inelastic soft X-ray scattering.

PubMed

Andresen, N C; Denes, P; Goldschmidt, A; Joseph, J; Karcher, A; Tindall, C S

2017-08-01

We have developed a charge-coupled device (CCD) with 5 μm × 45 μm pixels on high-resistivity silicon. The fully depleted 200 μm-thick silicon detector is back-illuminated through a 10 nm-thick in situ doped polysilicon window and is thus highly efficient for soft through >8 keV hard X-rays. The device described here is a 1.5 megapixel CCD with 2496 × 620 pixels. The pixel and camera geometry was optimized for Resonant Inelastic X-ray Scattering (RIXS) and is particularly advantageous for spectrometers with limited arm lengths. In this article, we describe the device architecture, construction and operation, and its performance during tests at the Advance Light Source (ALS) 8.0.1 RIXS beamline. The improved spectroscopic performance, when compared with a current standard commercial camera, is demonstrated with a ∼280 eV (C K ) X-ray beam on a graphite sample. Readout noise is typically 3-6 electrons and the point spread function for soft C K X-rays in the 5 μm direction is 4.0 μm ± 0.2 μm. The measured quantum efficiency of the CCD is greater than 75% in the range from 200 eV to 1 keV.

pt5m - a 0.5 m robotic telescope on La Palma

NASA Astrophysics Data System (ADS)

Hardy, L. K.; Butterley, T.; Dhillon, V. S.; Littlefair, S. P.; Wilson, R. W.

2015-12-01

pt5m is a 0.5 m robotic telescope located on the roof of the 4.2 m William Herschel Telescope (WHT) building, at the Roque de los Muchachos Observatory, La Palma. Using a five-position filter wheel and CCD detector, and bespoke control software, pt5m provides a high-quality robotic observing facility. The telescope first began robotic observing in 2012, and is now contributing to transient follow-up and time-resolved astronomical studies. In this paper, we present the scientific motivation behind pt5m, as well as the specifications and unique features of the facility. We also present an example of the science we have performed with pt5m, where we measure the radius of the transiting exoplanet WASP-33b. We find a planetary radius of 1.603 ± 0.014RJ.

Microgravity

NASA Image and Video Library

1991-04-03

The USML-1 Glovebox (GBX) is a multi-user facility supporting 16 experiments in fluid dynamics, combustion sciences, crystal growth, and technology demonstration. The GBX has an enclosed working space which minimizes the contamination risks to both Spacelab and experiment samples. The GBX supports four charge-coupled device (CCD) cameras (two of which may be operated simultaneously) with three black-and-white and three color camera CCD heads available. The GBX also has a backlight panel, a 35 mm camera, and a stereomicroscope that offers high-magnification viewing of experiment samples. Video data can also be downlinked in real-time. The GBX also provides electrical power for experiment hardware, a time-temperature display, and cleaning supplies.

Microgravity

NASA Image and Video Library

1995-08-29

The USML-1 Glovebox (GBX) is a multi-user facility supporting 16 experiments in fluid dynamics, combustion sciences, crystal growth, and technology demonstration. The GBX has an enclosed working space which minimizes the contamination risks to both Spacelab and experiment samples. The GBX supports four charge-coupled device (CCD) cameras (two of which may be operated simultaneously) with three black-and-white and three color camera CCD heads available. The GBX also has a backlight panel, a 35 mm camera, and a stereomicroscope that offers high-magnification viewing of experiment samples. Video data can also be downlinked in real-time. The GBX also provides electrical power for experiment hardware, a time-temperature display, and cleaning supplies.

High Latitude Ionospheric Radio Studies.

DTIC Science & Technology

1988-01-01

AL’TOSCI.ED RES.LTS BY A.R.TL!.S.T. LLCAR OCT 83 S11U2, 2393 :1::’L- 𔃽 -1 5 2 42 !) 1 ? 7 2 2 5.1 10* rOF2 70FI bIIF H’F2 103M ~ WIN :OES W’V -- !I 5.6...4C > % V) L C-C) CC) CCC CC) C C CCD C)CD CD C) C)D CD (CDCC) C)D CDC) CD LC DCD (D C ),C C D DA rH H-4H H I I I HCD CD HHH HH IC DC)CDCD C -A - Ir

Synergistic effect of dicarbollide anions in liquid-liquid extraction: a molecular dynamics study at the octanol-water interface.

PubMed

Chevrot, G; Schurhammer, R; Wipff, G

2007-04-28

We report a molecular dynamics study of chlorinated cobalt bis(dicarbollide) anions [(B(9)C(2)H(8)Cl(3))(2)Co](-)"CCD(-)" in octanol and at the octanol-water interface, with the main aim to understand why these hydrophobic species act as strong synergists in assisted liquid-liquid cation extraction. Neat octanol is quite heterogeneous and is found to display dual solvation properties, allowing to well solubilize CCD(-), Cs(+) salts in the form of diluted pairs or oligomers, without displaying aggregation. At the aqueous interface, octanol behaves as an amphiphile, forming either monolayers or bilayers, depending on the initial state and confinement conditions. In biphasic octanol-water systems, CCD(-) anions are found to mainly partition to the organic phase, thus attracting Cs(+) or even more hydrophilic counterions like Eu(3+) into that phase. The remaining CCD(-) anions adsorb at the interface, but are less surface active than at the chloroform interface. Finally, we compare the interfacial behavior of the Eu(BTP)(3)(3+) complex in the absence and in the presence of CCD(-) anions and extractant molecules. It is found that when the CCD(-)'s are concentrated enough, the complex is extracted to the octanol phase. Otherwise, it is trapped at the interface, attracted by water. These results are compared to those obtained with chloroform as organic phase and discussed in the context of synergistic effect of CCD(-) in liquid-liquid extraction, pointing to the importance of dual solvation properties of octanol and of the hydrophobic character of CCD(-) for synergistic extraction of cations.

Solid state high resolution multi-spectral imager CCD test phase

NASA Technical Reports Server (NTRS)

1973-01-01

The program consisted of measuring the performance characteristics of charge coupled linear imaging devices, and a study defining a multispectral imaging system employing advanced solid state photodetection techniques.

Study of physical and sound absorbing property of epoxy blended coir dust biocomposite

NASA Astrophysics Data System (ADS)

Nath, G.; Mishra, S. P.

2016-09-01

Reinforcement biocomposite has gained more attention recently due to its low cost, abundantly availability, low density, specific properties, easy method of separation, enhanced energy recovery, CO2 neutrality, biodegradability and recyclable in nature. As a waste product of coconut fruit, the coconut coir dust (CCD) obtained from the coconut husk. The biocomposite material prepared from the CCD modified with the proper blended solution with the help of ultrasonic technique. The study of adiabatic compressibility of acetone / water (70/30) worth its blending property for bleaching of CCD. The biocomposite material of CCD was prepared with epoxy resin. The different physical properties such as sound absorption coefficient, thermal conductivity and electrical conductivity were measured. The morphological study of biocomposite and measurement of sound absorption coefficient shows good evidence of sound absorbing characteristics of biocomposite of CCD. The sound absorption property of composite material shows a significant result where as the thermal conductivity and electrical conductivity executes a weak result. Thus biocomposite of CCD can acts as a good sound absorber and band conductor of heat and electric current.

A 128K-bit CCD buffer memory system

NASA Technical Reports Server (NTRS)

Siemens, K. H.; Wallace, R. W.; Robinson, C. R.

1976-01-01

A prototype system was implemented to demonstrate that CCD's can be applied advantageously to the problem of low power digital storage and particularly to the problem of interfacing widely varying data rates. 8K-bit CCD shift register memories were used to construct a feasibility model 128K-bit buffer memory system. Peak power dissipation during a data transfer is less than 7 W., while idle power is approximately 5.4 W. The system features automatic data input synchronization with the recirculating CCD memory block start address. Descriptions are provided of both the buffer memory system and a custom tester that was used to exercise the memory. The testing procedures and testing results are discussed. Suggestions are provided for further development with regards to the utilization of advanced versions of CCD memory devices to both simplified and expanded memory system applications.

Care for Child Development: an intervention in support of responsive caregiving and early child development.

PubMed

Lucas, J E; Richter, L M; Daelmans, B

2018-01-01

An estimated 43% of children younger than 5 years of age are at elevated risk of failing to achieve their human potential. In response, the World Health Organization and UNICEF developed Care for Child Development (CCD), based on the science of child development, to improve sensitive and responsive caregiving and promote the psychosocial development of young children. In 2015, the World Health Organization and UNICEF identified sites where CCD has been implemented and sustained. The sites were surveyed, and responses were followed up by phone interviews. Project reports provided information on additional sites, and a review of published studies was undertaken to document the effectiveness of CCD for improving child and family outcomes, as well as its feasibility for implementation in resource-constrained communities. The inventory found that CCD had been integrated into existing services in diverse sectors in 19 countries and 23 sites, including child survival, health, nutrition, infant day care, early education, family and child protection and services for children with disabilities. Published and unpublished evaluations have found that CCD interventions can improve child development, growth and health, as well as responsive caregiving. It has also been reported to reduce maternal depression, a known risk factor for poor pregnancy outcomes and poor child health, growth and development. Although CCD has expanded beyond initial implementation sites, only three countries reported having national policy support for integrating CCD into health or other services. Strong interest exists in many countries to move beyond child survival to protect and support optimal child development. The United Nations Sustainable Development Goals depend on children realizing their potential to build healthy and emotionally, cognitively and socially competent future generations. More studies are needed to guide the integration of the CCD approach under different conditions. Nevertheless, the time is right to provide for the scale-up of CCD as part of services for families and children. © 2017 The Authors. Child: Care, Health and Development Published by John Wiley & Sons Ltd.

Regulation of transport in the connecting tubule and cortical collecting duct.

PubMed

Staruschenko, Alexander

2012-04-01

The central goal of this overview article is to summarize recent findings in renal epithelial transport,focusing chiefly on the connecting tubule (CNT) and the cortical collecting duct (CCD).Mammalian CCD and CNT are involved in fine-tuning of electrolyte and fluid balance through reabsorption and secretion. Specific transporters and channels mediate vectorial movements of water and solutes in these segments. Although only a small percent of the glomerular filtrate reaches the CNT and CCD, these segments are critical for water and electrolyte homeostasis since several hormones, for example, aldosterone and arginine vasopressin, exert their main effects in these nephron sites. Importantly, hormones regulate the function of the entire nephron and kidney by affecting channels and transporters in the CNT and CCD. Knowledge about the physiological and pathophysiological regulation of transport in the CNT and CCD and particular roles of specific channels/transporters has increased tremendously over the last two decades.Recent studies shed new light on several key questions concerning the regulation of renal transport.Precise distribution patterns of transport proteins in the CCD and CNT will be reviewed, and their physiological roles and mechanisms mediating ion transport in these segments will also be covered. Special emphasis will be given to pathophysiological conditions appearing as a result of abnormalities in renal transport in the CNT and CCD. © 2012 American Physiological Society. Compr Physiol 2:1491-1539, 2012.

Cervical spine dysfunction signs and symptoms in individuals with temporomandibular disorder.

PubMed

Weber, Priscila; Corrêa, Eliane Castilhos Rodrigues; Ferreira, Fabiana dos Santos; Soares, Juliana Corrêa; Bolzan, Geovana de Paula; Silva, Ana Maria Toniolo da

2012-01-01

To study the frequency of cervical spine dysfunction (CCD) signs and symptoms in subjects with and without temporomandibular disorder (TMD) and to assess the craniocervical posture influence on TMD and CCD coexistence. Participants were 71 women (19 to 35 years), assessed about TMD presence; 34 constituted the TMD group (G1) and 37 comprised the group without TMD (G2). The CCD was evaluated through the Craniocervical Dysfunction Index and the Cervical Mobility Index. Subjects were also questioned about cervical pain. Craniocervical posture was assessed by cephalometric analysis. There was no difference in the craniocervical posture between groups. G2 presented more mild CCD frequency and less moderate and severe CCD frequency (p=0.01). G1 presented higher percentage of pain during movements (p=0.03) and pain during cervical muscles palpation (p=0.01) compared to G2. Most of the TMD patients (88.24%) related cervical pain with significant difference when compared to G2 (p=0.00). Craniocervical posture assessment showed no difference between groups, suggesting that postural alterations could be more related to the CCD. Presence of TMD resulted in higher frequency of cervical pain symptom. Thus the coexistence of CCD and TMD signs and symptoms appear to be more related to the common innervations of the trigeminocervical complex and hyperalgesia of the TMD patients than to craniocervical posture deviations.

Past, present, and future of sublimation transfer imaging

NASA Astrophysics Data System (ADS)

Akada, Masanori

1990-07-01

SONY's announcement of tlavica system shaked the world in 1981. In the new nonphotographic imaging system, image is acquired with CCD to be converted into electric image-signal, stored in magnetic recording media,displayed on a CR1 and printed on a special sheet. To get a hard copy, Sublimation Transfer technology was developed. That announcement brought about world-wide R&D of competitive color imaging systems: Ink-jet, Wax transfer,. Sublimation Transfer(ST) and Electrophotography. In spite of much effort,most of those were insufficient for getting a good hard copy. Developing sufficient ST recording media, Dai Nippon Printing started ST recording media business in 1986. It was the first manufacturing scale production and sale of ST recording media in the world. Nowadays ST technology is known for its advantages: high image quality, consistency from copy to copy, smooth tone-reproduction from high-light to maximum density, and easiness to use. In the following paper progress of ST recording media and the present situation and future markets of the media will be presented.

High-speed autoverifying technology for printed wiring boards

NASA Astrophysics Data System (ADS)

Ando, Moritoshi; Oka, Hiroshi; Okada, Hideo; Sakashita, Yorihiro; Shibutani, Nobumi

1996-10-01

We have developed an automated pattern verification technique. The output of an automated optical inspection system contains many false alarms. Verification is needed to distinguish between minor irregularities and serious defects. In the past, this verification was usually done manually, which led to unsatisfactory product quality. The goal of our new automated verification system is to detect pattern features on surface mount technology boards. In our system, we employ a new illumination method, which uses multiple colors and multiple direction illumination. Images are captured with a CCD camera. We have developed a new algorithm that uses CAD data for both pattern matching and pattern structure determination. This helps to search for patterns around a defect and to examine defect definition rules. These are processed with a high speed workstation and a hard-wired circuits. The system can verify a defect within 1.5 seconds. The verification system was tested in a factory. It verified 1,500 defective samples and detected all significant defects with only a 0.1 percent of error rate (false alarm).

So You Want a Meade LX Telescope!

NASA Astrophysics Data System (ADS)

Harris, Lawrence

Perhaps every generation of astronomers believes that their telescopes are the best that have ever been. They are surely all correct! The great leap of our time is that computer-designed and machined parts have led to more accurately made components that give the astronomer ever better views. The manual skills of the craftsman mirror grinder have been transformed into the new-age skills of the programmer and the machine maker. (The new products did not end the work of craftsman telescope makers, though. Many highly skilled amateur/professional opticians continued to produce good-quality mirrors that are still seen today.) Amateur-priced telescopes are now capable of highly accurate tracking and computer control that were once only the province of professionals. This has greatly increased the possibilities of serious astronomy projects for which tailor-made software has been developed. Add a CCD camera to these improved telescopes (see Chap. 3), and you bring a whole new dimension to your astronomy (see Fig. 1.1).

Super fast detection of latent fingerprints with water soluble CdTe quantum dots.

PubMed

Cai, Kaiyang; Yang, Ruiqin; Wang, Yanji; Yu, Xuejiao; Liu, Jianjun

2013-03-10

A new method based on the use of highly fluorescent water-soluble cadmium telluride (CdTe) quantum dots (QDs) capped with mercaptosuccinic acid (MSA) was explored to develop latent fingerprints. After optimized the effectiveness of QDs method contains pH value and developing time, super fast detection was achieved. Excellent fingerprint images were obtained in 1-3s after immersed the latent fingerprints into quantum dots solution on various non-porous surfaces, i.e. adhesive tape, transparent tape, aluminum foil and stainless steel. High sensitivity of the new latent fingerprints develop method was obtained by developing the fingerprints pressed on aluminum foil successively with the same finger. Compared with methyl violet and rhodamine 6G, the MSA-CdTe QDs showed the higher develop speed and fingerprint image quality. Clear image can be maintained for months by extending exposure time of CCD camera, storing fingerprints in a low temperature condition and secondary development. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Instructional Staff Salary and Benefits Spending: 1991-2011. Data Point. NCES 2016-156

ERIC Educational Resources Information Center

Malkus, Nat; Hoyer, Kathleen Mulvaney

2016-01-01

This report uses expenditure data from the Common Core of Data (CCD) "National Public Education Financial Survey (NPEFS)" for fiscal years 1991 to 2011 and instructional staff data from the CCD "State Nonfiscal Survey of Public Elementary/Secondary Education" for school years 1990-91 to 2010-11. The CCD annually collects fiscal…

Specialized CCDs for high-frame-rate visible imaging and UV imaging applications

NASA Astrophysics Data System (ADS)

Levine, Peter A.; Taylor, Gordon C.; Shallcross, Frank V.; Tower, John R.; Lawler, William B.; Harrison, Lorna J.; Socker, Dennis G.; Marchywka, Mike

1993-11-01

This paper reports recent progress by the authors in two distinct charge coupled device (CCD) technology areas. The first technology area is high frame rate, multi-port, frame transfer imagers. A 16-port, 512 X 512, split frame transfer imager and a 32-port, 1024 X 1024, split frame transfer imager are described. The thinned, backside illuminated devices feature on-chip correlated double sampling, buried blooming drains, and a room temperature dark current of less than 50 pA/cm2, without surface accumulation. The second technology area is vacuum ultraviolet (UV) frame transfer imagers. A developmental 1024 X 640 frame transfer imager with 20% quantum efficiency at 140 nm is described. The device is fabricated in a p-channel CCD process, thinned for backside illumination, and utilizes special packaging to achieve stable UV response.

Detection of defects in laser welding of AZ31B magnesium alloy in zero-gap lap joint configuration by a real-time spectroscopic analysis

NASA Astrophysics Data System (ADS)

Harooni, Masoud; Carlson, Blair; Kovacevic, Radovan

2014-05-01

The effect of surface oxide layer existing at the lap-joint faying surface of magnesium sheets is investigated on the keyhole dynamics of the weld pool and weld bead qualities. It is observed that by removing the oxide layer from the faying surface of the lap joint, a high quality weld can be achieved in the laser welding process. However, the presence of an oxide layer deteriorates the quality of the weld by forming pores at the interface of the two overlapped sheets. The purpose of this paper is to identify the correlation between the integrity of the weld and the interaction between the laser and material. A spectroscopy sensor was applied to detect the spectra emitted from a plasma plume during the laser welding of AZ31B magnesium alloy in a zero-gap lap joint configuration. The electron temperature was calculated by applying a Boltzmann plot method based on the detected spectra, and the correlation between the pore formation and the spectral signals was studied. The laser molten pool and the keyhole condition were monitored in real-time by a high speed charge-coupled device (CCD) camera. A green laser was used as an illumination source in order to detect the influence of the oxide layer on the dynamic behavior of the molten pool. Results revealed that the detected spectrum and weld defects had a meaningful correlation for real-time monitoring of the weld quality during laser welding of magnesium alloys.

[Techniques for pixel response nonuniformity correction of CCD in interferential imaging spectrometer].

PubMed

Yao, Tao; Yin, Shi-Min; Xiangli, Bin; Lü, Qun-Bo

2010-06-01

Based on in-depth analysis of the relative radiation scaling theorem and acquired scaling data of pixel response nonuniformity correction of CCD (charge-coupled device) in spaceborne visible interferential imaging spectrometer, a pixel response nonuniformity correction method of CCD adapted to visible and infrared interferential imaging spectrometer system was studied out, and it availably resolved the engineering technical problem of nonuniformity correction in detector arrays for interferential imaging spectrometer system. The quantitative impact of CCD nonuniformity on interferogram correction and recovery spectrum accuracy was given simultaneously. Furthermore, an improved method with calibration and nonuniformity correction done after the instrument is successfully assembled was proposed. The method can save time and manpower. It can correct nonuniformity caused by other reasons in spectrometer system besides CCD itself's nonuniformity, can acquire recalibration data when working environment is changed, and can also more effectively improve the nonuniformity calibration accuracy of interferential imaging

Familial cleidocranial dysplasia misdiagnosed as rickets over three generations.

PubMed

Franceschi, Roberto; Maines, Evelina; Fedrizzi, Michela; Piemontese, Maria Rosaria; De Bonis, Patrizia; Agarwal, Nivedita; Bellizzi, Maria; Di Palma, Annunziata

2015-10-01

Cleidocranial dysplasia (CCD) is a rare autosomal dominant skeletal dysplasia characterized by hypoplastic clavicles, late closure of the fontanels, dental problems and other skeletal features. CCD is caused by mutations, deletions or duplications in runt-related transcription factor 2 (RUNX2), which encodes for a protein essential for osteoblast differentiation and chondrocyte maturation. We describe three familial cases of CCD, misdiagnosed as rickets over three generations. No mutations were detected on standard DNA sequencing of RUNX2, but a novel deletion was identified on quantitative polymerase chain reaction (qPCR) and multiple ligation-dependent probe amplification (MLPA). The present cases indicate that CCD could be misdiagnosed as rickets, leading to inappropriate treatment, and confirm that mutations in RUNX2 are not able to be identified on standard DNA sequencing in all CCD patients, but can be identified on qPCR and MLPA. © 2015 Japan Pediatric Society.

High accuracy dynamic highway mapping using a GPS/INS/CCD system with on-the-fly GPS ambiguity resolution : executive summary.

DOT National Transportation Integrated Search

2004-09-01

Conventionally, the road centerline surveys have : been performed by the traditional survey methods, : providing rather high, even sub-centimeter level of : accuracy. The major problem, however, that the : Departments of Transportation face, is the s...

The climate hazards infrared precipitation with stations—a new environmental record for monitoring extremes

USGS Publications Warehouse

Funk, Chris; Peterson, Pete; Landsfeld, Martin; Pedreros, Diego; Verdin, James; Shukla, Shraddhanand; Husak, Gregory; Rowland, James; Harrison, Laura; Hoell, Andrew; Michaelsen, Joel

2015-01-01

The Climate Hazards group Infrared Precipitation with Stations (CHIRPS) dataset builds on previous approaches to ‘smart’ interpolation techniques and high resolution, long period of record precipitation estimates based on infrared Cold Cloud Duration (CCD) observations. The algorithm i) is built around a 0.05° climatology that incorporates satellite information to represent sparsely gauged locations, ii) incorporates daily, pentadal, and monthly 1981-present 0.05° CCD-based precipitation estimates, iii) blends station data to produce a preliminary information product with a latency of about 2 days and a final product with an average latency of about 3 weeks, and iv) uses a novel blending procedure incorporating the spatial correlation structure of CCD-estimates to assign interpolation weights. We present the CHIRPS algorithm, global and regional validation results, and show how CHIRPS can be used to quantify the hydrologic impacts of decreasing precipitation and rising air temperatures in the Greater Horn of Africa. Using the Variable Infiltration Capacity model, we show that CHIRPS can support effective hydrologic forecasts and trend analyses in southeastern Ethiopia.

Spatial resolution of a hard x-ray CCD detector.

PubMed

Seely, John F; Pereira, Nino R; Weber, Bruce V; Schumer, Joseph W; Apruzese, John P; Hudson, Lawrence T; Szabo, Csilla I; Boyer, Craig N; Skirlo, Scott

2010-08-10

The spatial resolution of an x-ray CCD detector was determined from the widths of the tungsten x-ray lines in the spectrum formed by a crystal spectrometer in the 58 to 70 keV energy range. The detector had 20 microm pixel, 1700 by 1200 pixel format, and a CsI x-ray conversion scintillator. The spectral lines from a megavolt x-ray generator were focused on the spectrometer's Rowland circle by a curved transmission crystal. The line shapes were Lorentzian with an average width after removal of the natural and instrumental line widths of 95 microm (4.75 pixels). A high spatial frequency background, primarily resulting from scattered gamma rays, was removed from the spectral image by Fourier analysis. The spectral lines, having low spatial frequency in the direction perpendicular to the dispersion, were enhanced by partially removing the Lorentzian line shape and by fitting Lorentzian curves to broad unresolved spectral features. This demonstrates the ability to improve the spectral resolution of hard x-ray spectra that are recorded by a CCD detector with well-characterized intrinsic spatial resolution.

The astro-geodetic use of CCD for gravity field refinement

NASA Astrophysics Data System (ADS)

Gerstbach, G.

1996-07-01

The paper starts with a review of geoid projects, where vertical deflections are more effective than gravimetry. In alpine regions the economy of astrogeoids is at least 10 times higher, but many countries do not make use of this fact - presumably because the measurements are not fully automated up to now. Based upon the experiences of astrometry of high satellites and own tests the author analyses the use of CCD for astro-geodetic measurements. Automation and speeding up will be possible in a few years, the latter depending on the observation scheme. Sensor characteristics, cooling and reading out of the devices should be harmonized. Using line sensors in small prism astrolabes, the CCD accuracy will reach the visual one (±0.2″) within 5-10 years. Astrogeoids can be combined ideally with geological data, because vertical variation of rock densities does not cause systematic effects (contrary to gravimetry). So a geoid of ±5 cm accuracy (achieved in Austria and other alpine countries by 5-10 points per 1000 km 2) can be improved to ±2 cm without additional observations and border effects.

The climate hazards infrared precipitation with stations—a new environmental record for monitoring extremes

PubMed Central

Funk, Chris; Peterson, Pete; Landsfeld, Martin; Pedreros, Diego; Verdin, James; Shukla, Shraddhanand; Husak, Gregory; Rowland, James; Harrison, Laura; Hoell, Andrew; Michaelsen, Joel

2015-01-01

The Climate Hazards group Infrared Precipitation with Stations (CHIRPS) dataset builds on previous approaches to ‘smart’ interpolation techniques and high resolution, long period of record precipitation estimates based on infrared Cold Cloud Duration (CCD) observations. The algorithm i) is built around a 0.05° climatology that incorporates satellite information to represent sparsely gauged locations, ii) incorporates daily, pentadal, and monthly 1981-present 0.05° CCD-based precipitation estimates, iii) blends station data to produce a preliminary information product with a latency of about 2 days and a final product with an average latency of about 3 weeks, and iv) uses a novel blending procedure incorporating the spatial correlation structure of CCD-estimates to assign interpolation weights. We present the CHIRPS algorithm, global and regional validation results, and show how CHIRPS can be used to quantify the hydrologic impacts of decreasing precipitation and rising air temperatures in the Greater Horn of Africa. Using the Variable Infiltration Capacity model, we show that CHIRPS can support effective hydrologic forecasts and trend analyses in southeastern Ethiopia. PMID:26646728

An evaluation of an ICCD imager of dynamic range expansion technique and application of insitu procedures for life-time extension

NASA Technical Reports Server (NTRS)

Currie, D. G.

1982-01-01

Research toward practical implementation of the Intensified Charge Coupled Device (ICCD) as a photon-counting array detector for astronomy is reported. The first area of concentration was to determine the rate and extent of the lifetime limiting damage to the CCD caused by the impact of high energy electrons, and to find whether various methods of annealing the damage were productive. The second effort was to determine the performance of the ICCD in a photon-counting mode to produce extended dynamic range measurements. There are two main effects that appear as the practical results of the electron damage to the CCD. One is an increase in the leakage current, i.e., the normal thermal generation of charge carriers in the silicon that provides a background dark signal that adds to the light produced image. In an undamaged CCD, the leakage current is usually fairly uniform across the photosensitive area of the silicon chip, with the exception of various bright pixels which have an anomalous leakage current well above the overall level.

Hyperspectral Image-Based Night-Time Vehicle Light Detection Using Spectral Normalization and Distance Mapper for Intelligent Headlight Control.

PubMed

Kim, Heekang; Kwon, Soon; Kim, Sungho

2016-07-08

This paper proposes a vehicle light detection method using a hyperspectral camera instead of a Charge-Coupled Device (CCD) or Complementary metal-Oxide-Semiconductor (CMOS) camera for adaptive car headlamp control. To apply Intelligent Headlight Control (IHC), the vehicle headlights need to be detected. Headlights are comprised from a variety of lighting sources, such as Light Emitting Diodes (LEDs), High-intensity discharge (HID), and halogen lamps. In addition, rear lamps are made of LED and halogen lamp. This paper refers to the recent research in IHC. Some problems exist in the detection of headlights, such as erroneous detection of street lights or sign lights and the reflection plate of ego-car from CCD or CMOS images. To solve these problems, this study uses hyperspectral images because they have hundreds of bands and provide more information than a CCD or CMOS camera. Recent methods to detect headlights used the Spectral Angle Mapper (SAM), Spectral Correlation Mapper (SCM), and Euclidean Distance Mapper (EDM). The experimental results highlight the feasibility of the proposed method in three types of lights (LED, HID, and halogen).

Fast, high-resolution 3D dosimetry utilizing a novel optical-CT scanner incorporating tertiary telecentric collimation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sakhalkar, H. S.; Oldham, M.

2008-01-15

This study introduces a charge coupled device (CCD) area detector based optical-computed tomography (optical-CT) scanner for comprehensive verification of radiation dose distributions recorded in nonscattering radiochromic dosimeters. Defining characteristics include: (i) a very fast scanning time of {approx}5 min to acquire a complete three-dimensional (3D) dataset, (ii) improved image formation through the use of custom telecentric optics, which ensures accurate projection images and minimizes artifacts from scattered and stray-light sources, and (iii) high resolution (potentially 50 {mu}m) isotropic 3D dose readout. The performance of the CCD scanner for 3D dose readout was evaluated by comparison with independent 3D readout frommore » the single laser beam OCTOPUS-scanner for the same PRESAGE dosimeters. The OCTOPUS scanner was considered the 'gold standard' technique in light of prior studies demonstrating its accuracy. Additional comparisons were made against calculated dose distributions from the ECLIPSE treatment-planning system. Dose readout for the following treatments were investigated: (i) a single rectangular beam irradiation to investigate small field and very steep dose gradient dosimetry away from edge effects, (ii) a 2-field open beam parallel-opposed irradiation to investigate dosimetry along steep dose gradients, and (iii) a 7-field intensity modulated radiation therapy (IMRT) irradiation to investigate dosimetry for complex treatment delivery involving modulation of fluence and for dosimetry along moderate dose gradients. Dose profiles, dose-difference plots, and gamma maps were employed to evaluate quantitative estimates of agreement between independently measured and calculated dose distributions. Results indicated that dose readout from the CCD scanner was in agreement with independent gold-standard readout from the OCTOPUS-scanner as well as the calculated ECLIPSE dose distribution for all treatments, except in regions within a few millimeters of the edge of the dosimeter, where edge artifact is predominant. Agreement of line profiles was observed, even along steep dose gradients. Dose difference plots indicated that the CCD scanner dose readout differed from the OCTOPUSscanner readout and ECLIPSE calculations by {approx}10% along steep dose gradients and by {approx}5% along moderate dose gradients. Gamma maps (3% dose-difference and 3 mm distance-to-agreement acceptance criteria) revealed agreement, except for regions within 5 mm of the edge of the dosimeter where the edge artifact occurs. In summary, the data demonstrate feasibility of using the fast, high-resolution CCD scanner for comprehensive 3D dosimetry in all applications, except where dose readout is required close to the edges of the dosimeter. Further work is ongoing to reduce this artifact.« less

Updates on the Performance and Calibration of HST/STIS

NASA Astrophysics Data System (ADS)

Lockwood, Sean A.; Monroe, TalaWanda R.; Ogaz, Sara; Branton, Doug; Carlberg, Joleen K.; Debes, John H.; Jedrzejewski, Robert I.; Proffitt, Charles R.; Riley, Allyssa; Sohn, Sangmo Tony; Sonnentrucker, Paule; Walborn, Nolan R.; Welty, Daniel

2018-06-01

The Space Telescope Imaging Spectrograph (STIS) on the Hubble Space Telescope (HST) has been in orbit for 21 years and continues to produce high quality scientific results using a diverse complement of operating modes. These include spatially resolved spectroscopy in the UV and optical, high spatial resolution echelle spectroscopy in the UV, and solar-blind imaging in the UV. In addition, STIS possesses unique visible-light coronagraphic modes that keep the instrument at the forefront of exoplanet and debris-disk research. As the instrument's characteristics evolve over its lifetime, the instrument team at the Space Telescope Science Institute monitors its performance and works towards improving the quality of its data products. Here we present updates on the status of the STIS CCD and FUV & NUV MAMA detectors, as well as changes to the CalSTIS reduction pipeline. We also discuss progress toward the recalibration of the E140M/1425 echelle mode. The E140M grating blaze function shapes have changed since flux calibration was carried out following SM4, which limits the relative photometric flux accuracy of some spectral orders up to 5-10% at the edges. In Cycle 25 a special calibration program was executed to obtain updated sensitivity curves for the E140M/1425 setting.

Image deblurring using a joint entropy prior in x-ray luminescence computed tomography

NASA Astrophysics Data System (ADS)

Su, Chang; Dutta, Joyita; Zhang, Hui; El Fakhri, Georges; Li, Quanzheng

2017-03-01

X-ray luminescence computed tomography (XLCT) is an emerging hybrid imaging modality that can provide functional and anatomical images at the same time. Traditional narrow beam XLCT can achieve high spatial resolution as well as high sensitivity. However, by treating the CCD camera as a single pixel detector, this kind of scheme resembles the first generation of CT scanner which results in a long scanning time and a high radiation dose. Although cone beam or fan beam XLCT has the ability to mitigate this problem with an optical propagation model introduced, image quality is affected because the inverse problem is ill-conditioned. Much effort has been done to improve the image quality through hardware improvements or by developing new reconstruction techniques for XLCT. The objective of this work is to further enhance the already reconstructed image by introducing anatomical information through retrospective processing. The deblurring process used a spatially variant point spread function (PSF) model and a joint entropy based anatomical prior derived from a CT image acquired using the same XLCT system. A numerical experiment was conducted with a real mouse CT image from the Digimouse phantom used as the anatomical prior. The resultant images of bone and lung regions showed sharp edges and good consistency with the CT image. Activity error was reduced by 52.3% even for nanophosphor lesion size as small as 0.8mm.

The Structural Biology Center 19ID undulator beamline: facility specifications and protein crystallographic results

PubMed Central

Rosenbaum, Gerd; Alkire, Randy W.; Evans, Gwyndaf; Rotella, Frank J.; Lazarski, Krzystof; Zhang, Rong-Guang; Ginell, Stephan L.; Duke, Norma; Naday, Istvan; Lazarz, Jack; Molitsky, Michael J.; Keefe, Lisa; Gonczy, John; Rock, Larry; Sanishvili, Ruslan; Walsh, Martin A.; Westbrook, Edwin; Joachimiak, Andrzej

2008-01-01

The 19ID undulator beamline of the Structure Biology Center has been designed and built to take full advantage of the high flux, brilliance and quality of X-ray beams delivered by the Advanced Photon Source. The beamline optics are capable of delivering monochromatic X-rays with photon energies from 3.5 to 20 keV (3.5–0.6 Å wavelength) with fluxes up to 8–18 × 1012 photons s−1 (depending on photon energy) onto cryogenically cooled crystal samples. The size of the beam (full width at half-maximum) at the sample position can be varied from 2.2 mm × 1.0 mm (horizontal × vertical, unfocused) to 0.083 mm × 0.020 mm in its fully focused configuration. Specimen-to-detector distances of between 100 mm and 1500 mm can be used. The high flexibility, inherent in the design of the optics, coupled with a κ-geometry goniometer and beamline control software allows optimal strategies to be adopted in protein crystallographic experiments, thus maximizing the chances of their success. A large-area mosaic 3 × 3 CCD detector allows high-quality diffraction data to be measured rapidly to the crystal diffraction limits. The beamline layout and the X-ray optical and endstation components are described in detail, and the results of representative crystallographic experiments are presented. PMID:16371706

Design of measuring system for wire diameter based on sub-pixel edge detection algorithm

NASA Astrophysics Data System (ADS)

Chen, Yudong; Zhou, Wang

2016-09-01

Light projection method is often used in measuring system for wire diameter, which is relatively simpler structure and lower cost, and the measuring accuracy is limited by the pixel size of CCD. Using a CCD with small pixel size can improve the measuring accuracy, but will increase the cost and difficulty of making. In this paper, through the comparative analysis of a variety of sub-pixel edge detection algorithms, polynomial fitting method is applied for data processing in measuring system for wire diameter, to improve the measuring accuracy and enhance the ability of anti-noise. In the design of system structure, light projection method with orthogonal structure is used for the detection optical part, which can effectively reduce the error caused by line jitter in the measuring process. For the electrical part, ARM Cortex-M4 microprocessor is used as the core of the circuit module, which can not only drive double channel linear CCD but also complete the sampling, processing and storage of the CCD video signal. In addition, ARM microprocessor can complete the high speed operation of the whole measuring system for wire diameter in the case of no additional chip. The experimental results show that sub-pixel edge detection algorithm based on polynomial fitting can make up for the lack of single pixel size and improve the precision of measuring system for wire diameter significantly, without increasing hardware complexity of the entire system.

Earth elevation map production and high resolution sensing camera imaging analysis

NASA Astrophysics Data System (ADS)

Yang, Xiubin; Jin, Guang; Jiang, Li; Dai, Lu; Xu, Kai

2010-11-01

The Earth's digital elevation which impacts space camera imaging has prepared and imaging has analysed. Based on matching error that TDI CCD integral series request of the speed of image motion, statistical experimental methods-Monte Carlo method is used to calculate the distribution histogram of Earth's elevation in image motion compensated model which includes satellite attitude changes, orbital angular rate changes, latitude, longitude and the orbital inclination changes. And then, elevation information of the earth's surface from SRTM is read. Earth elevation map which produced for aerospace electronic cameras is compressed and spliced. It can get elevation data from flash according to the shooting point of latitude and longitude. If elevation data between two data, the ways of searching data uses linear interpolation. Linear interpolation can better meet the rugged mountains and hills changing requests. At last, the deviant framework and camera controller are used to test the character of deviant angle errors, TDI CCD camera simulation system with the material point corresponding to imaging point model is used to analyze the imaging's MTF and mutual correlation similarity measure, simulation system use adding cumulation which TDI CCD imaging exceeded the corresponding pixel horizontal and vertical offset to simulate camera imaging when stability of satellite attitude changes. This process is practicality. It can effectively control the camera memory space, and meet a very good precision TDI CCD camera in the request matches the speed of image motion and imaging.

A multi-characteristic based algorithm for classifying vegetation in a plateau area: Qinghai Lake watershed, northwestern China

NASA Astrophysics Data System (ADS)

Ma, Weiwei; Gong, Cailan; Hu, Yong; Li, Long; Meng, Peng

2015-10-01

Remote sensing technology has been broadly recognized for its convenience and efficiency in mapping vegetation, particularly in high-altitude and inaccessible areas where there are lack of in-situ observations. In this study, Landsat Thematic Mapper (TM) images and Chinese environmental mitigation satellite CCD sensor (HJ-1 CCD) images, both of which are at 30m spatial resolution were employed for identifying and monitoring of vegetation types in a area of Western China——Qinghai Lake Watershed(QHLW). A decision classification tree (DCT) algorithm using multi-characteristic including seasonal TM/HJ-1 CCD time series data combined with digital elevation models (DEMs) dataset, and a supervised maximum likelihood classification (MLC) algorithm with single-data TM image were applied vegetation classification. Accuracy of the two algorithms was assessed using field observation data. Based on produced vegetation classification maps, it was found that the DCT using multi-season data and geomorphologic parameters was superior to the MLC algorithm using single-data image, improving the overall accuracy by 11.86% at second class level and significantly reducing the "salt and pepper" noise. The DCT algorithm applied to TM /HJ-1 CCD time series data geomorphologic parameters appeared as a valuable and reliable tool for monitoring vegetation at first class level (5 vegetation classes) and second class level(8 vegetation subclasses). The DCT algorithm using multi-characteristic might provide a theoretical basis and general approach to automatic extraction of vegetation types from remote sensing imagery over plateau areas.

Mesozoic Alpine facies deposition as a result of past latitudinal plate motion.

PubMed

Muttoni, Giovanni; Erba, Elisabetta; Kent, Dennis V; Bachtadse, Valerian

2005-03-03

The fragmentation of Pangaea as a consequence of the opening of the Atlantic Ocean is documented in the Alpine-Mediterranean region by the onset of widespread pelagic sedimentation. Shallow-water sediments were replaced by mainly pelagic limestones in the Early Jurassic period, radiolarian cherts in the Middle-Late Jurassic period, and again pelagic limestones in the Late Jurassic-Cretaceous period. During initial extension, basin subsidence below the carbonate compensation depth (CCD) is thought to have triggered the transition from Early Jurassic limestones to Middle-Late Jurassic radiolarites. It has been proposed that the transition from radiolarites to limestones in the Late Jurassic period was due to an increase in calcareous nannoplankton abundance when the CCD was depressed below the ocean floor. But in modern oceans, sediments below the CCD are not necessarily radiolaritic. Here we present palaeomagnetic samples from the Jurassic-Cretaceous pelagic succession exposed in the Lombardian basin, Italy. On the basis of an analysis of our palaeolatitudinal data in a broader palaeogeographic context, we propose an alternative explanation for the above facies tripartition. We suggest that the Lombardian basin drifted initially towards, and subsequently away from, a near-equatorial upwelling zone of high biosiliceous productivity. Our tectonic model for the genesis of radiolarites adds an essential horizontal plate motion component to explanations involving only vertical variations of CCD relative to the ocean floor. It may explain the deposition of radiolarites throughout the Mediterranean and Middle Eastern region during the Jurassic period.

Plane development of lateral surfaces for inspection systems

NASA Astrophysics Data System (ADS)

Francini, F.; Fontani, D.; Jafrancesco, D.; Mercatelli, L.; Sansoni, P.

2006-08-01

The problem of developing the lateral surfaces of a 3D object can arise in item inspection using automated imaging systems. In an industrial environment, these control systems typically work at high rate and they have to assure a reliable inspection of the single item. For compactness requirements it is not convenient to utilise three or four CCD cameras to control all the lateral surfaces of an object. Moreover it is impossible to mount optical components near the object if it is placed on a conveyor belt. The paper presents a system that integrates on a single CCD picture the images of both the frontal surface and the lateral surface of an object. It consists of a freeform lens mounted in front of a CCD camera with a commercial lens. The aim is to have a good magnification of the lateral surface, maintaining a low aberration level for exploiting the pictures in an image processing software. The freeform lens, made in plastics, redirects the light coming from the object to the camera lens. The final result is to obtain on the CCD: - the frontal and lateral surface images, with a selected magnification (even with two different values for the two images); - a gap between these two images, so an automatic method to analyse the images can be easily applied. A simple method to design the freeform lens is illustrated. The procedure also allows to obtain the imaging system modifying a current inspection system reducing the cost.

Integration of Cell Phone Imaging with Microchip ELISA to Detect Ovarian Cancer HE4 Biomarker in Urine at the Point-of-Care

PubMed Central

Wang, ShuQi; Zhao, Xiaohu; Khimji, Imran; Akbas, Ragip; Qiu, Weiliang; Edwards, Dale; Cramer, Daniel W.; Ye, Bin; Demirci, Utkan

2013-01-01

Ovarian cancer is asymptomatic at early stages and most patients present with advanced levels of disease. Lack of cost-effective methods that can achieve frequent, simple and non-invasive testing hinders early detection and causes high mortality in ovarian cancer patients. Here, we report a simple and inexpensive microchip ELISA-based detection module that employs a portable detection system, i.e., a cell phone/charge-coupled device (CCD) to quantify an ovarian cancer biomarker, HE4, in urine. Integration of a mobile application with a cell phone enabled immediate processing of microchip ELISA results, which eliminated the need for a bulky, expensive spectrophotometer. The HE4 level detected by a cell phone or a lensless CCD system was significantly elevated in urine samples from cancer patients (n = 19) than normal healthy controls (n = 20) (p < 0.001). Receiver operating characteristic (ROC) analyses showed that the microchip ELISA coupled with a cell phone running an automated analysis application had a sensitivity of 89.5% at a specificity of 90%. Under the same specificity, the microchip ELISA coupled with a CCD had a sensitivity of 84.2%. In conclusion, integration of microchip ELISA with cell phone/CCD-based colorimetric measurement technology can be used to detect HE4 biomarker at the point-of-care (POC), paving the way to create bedside technologies for diagnostics and treatment monitoring. PMID:21881677

Heat dissipation schemes in QCLs monitored by CCD thermoreflectance (Conference Presentation)

NASA Astrophysics Data System (ADS)

Pierscinski, Kamil; Pierścińska, Dorota; Morawiec, Magdalena; Gutowski, Piotr; Karbownik, Piotr; Serebrennikova, Olga; Bugajski, Maciej

2017-02-01

In this paper we present the development of the instrumentation for accurate evaluation of the thermal characteristics of quantum cascade lasers based on CCD thermoreflectance (CCD TR). This method allows rapid thermal characterization of QCLs, as the registration of high-resolution map of the whole device facet lasts only several seconds. The capabilities of the CCD TR are used to study temperature dissipation schemes in different designs of QCLs. We report on the investigation of thermal performance of QCLs developed at the Institute of Electron Technology, with an emphasis on the influence of different material system, processing technology and device designs. We investigate and compare AlInAs/InGaAs/InP QCLs (lattice matched and strain compensated) of different architectures, i.e., double trench and buried heterostructure (BH) in terms of thermal management. Experimental results are in very good agreement with numerical predictions of heat dissipation in various device constructions. Numerical model is based on FEM model solved by commercial software package. The model assumes anisotropic thermal conductivity in the AR layers as well as the temperature dependence of thermal conductivities of all materials in the project. We have observed experimentally improvement of thermal properties of devices based on InP materials, especially for buried heterostructure type. The use of buried heterostructure enhanced the lateral heat dissipation from the active region of QCLs. The BH structure and epilayer-down bonding help dissipate the heat generated from active core of the QCL.

Isolation Mounting for Charge-Coupled Devices

NASA Technical Reports Server (NTRS)

Goss, W. C.; Salomon, P. M.

1985-01-01

CCD's suspended by wires under tension. Remote thermoelectric cooling of charge coupled device allows vibration isolating mounting of CCD assembly alone, without having to suspend entire mass and bulk of thermoelectric module. Mounting hardware simple and light. Developed for charge-coupled devices (CCD's) in infrared telescope support adaptable to sensors in variety of environments, e.g., sensors in nuclear reactors, engine exhausts and plasma chambers.

Common Core of Data (CCD): School Years 1996-1997 through 2000-2001. [CD-ROM].

ERIC Educational Resources Information Center

National Center for Education Statistics (ED), Washington, DC.

The Common Core of Data (CCD) is NCES's primary database on elementary and secondary public education in the United States. CCD is a comprehensive, annual, national statistical database of all elementary and secondary schools and school districts, which contains data that are comparable across all states. The 50 states and the District of Columbia…

Design, construction, and evaluation of new high resolution medical imaging detector/systems

NASA Astrophysics Data System (ADS)

Jain, Amit

Increasing need of minimally invasive endovascular image guided interventional procedures (EIGI) for accurate and successful treatment of vascular disease has set a quest for better image quality. Current state of the art detectors are not up to the mark for these complex procedures due to their inherent limitations. Our group has been actively working on the design and construction of a high resolution, region of interest CCD-based X-ray imager for some time. As a part of that endeavor, a Micro-angiographic fluoroscope (MAF) was developed to serve as a high resolution, ROI X-ray imaging detector in conjunction with large lower resolution full field of view (FOV) state-of-the-art x-ray detectors. The newly developed MAF is an indirect x-ray imaging detector capable of providing real-time images with high resolution, high sensitivity, no lag and low instrumentation noise. It consists of a CCD camera coupled to a light image intensifier (LII) through a fiber optic taper. The CsI(Tl) phosphor serving as the front end is coupled to the LII. For this work, the MAF was designed and constructed. The linear system cascade theory was used to evaluate the performance theoretically. Linear system metrics such as MTF and DQE were used to gauge the detector performance experimentally. The capabilities of the MAF as a complete system were tested using generalized linear system metrics. With generalized linear system metrics the effects of finite size focal spot, geometric magnification and the presence of scatter are included in the analysis and study. To minimize the effect of scatter, an anti-scatter grid specially designed for the MAF was also studied. The MAF was compared with the flat panel detector using signal-to-noise ratio and the two dimensional linear system metrics. The signal-to-noise comparison was carried out to point out the effect of pixel size and Point Spread Function of the detector. The two dimensional linear system metrics were used to investigate the comparative performance of both the detectors in similar simulated clinical neuro-vascular conditions. The last part of this work presents a unique quality of the MAF: operation in single photon mode. The successful operation of the MAF was demonstrated with considerable improvement in spatial and contrast resolution over conventional energy integrating mode. The work presented shows the evolution of a high resolution, high sensitivity, and region of interest x-ray imaging detector as an attractive and capable x-ray imager for the betterment of complex EIGI procedures. The capability of single photon counting mode imaging provides the potential for additional uses of the MAF including the possibility of use in dual modality imaging with radionuclide sources as well as x-rays.

Adolescent-onset alcohol abuse exacerbates the influence of childhood conduct disorder on late adolescent and early adult antisocial behaviour.

PubMed

Howard, Richard; Finn, Peter; Jose, Paul; Gallagher, Jennifer

2011-12-16

This study tested the hypothesis that adolescent-onset alcohol abuse (AOAA) would both mediate and moderate the effect of childhood conduct disorder on antisocial behaviour in late adolescence and early adulthood. A sample comprising 504 young men and women strategically recruited from the community were grouped using the criteria of the Diagnostic and Statistical Manual (DSM-IV, American Psychiatric Association. (1994). Diagnostic and statistical manual of mental disorders (4th ed.). Washington, DC: APA), as follows: neither childhood conduct disorder (CCD) nor alcohol abuse/dependence; CCD but no alcohol abuse or dependence; alcohol abuse/dependence but no CCD; both CCD and alcohol abuse/dependence. The outcome measure was the sum of positive responses to 55 interview items capturing a variety of antisocial behaviours engaged in since age 15. Severity of lifetime alcohol-related and CCD problems served as predictor variables in regression analysis. Antisocial behaviour problems were greatest in individuals with a history of co-occurring conduct disorder (CD) and alcohol abuse/dependence. While CCD was strongly predictive of adult antisocial behaviour, this effect was both mediated and moderated (exacerbated) by AOAA.

Combinations of coupled cluster, density functionals, and the random phase approximation for describing static and dynamic correlation, and van der Waals interactions

NASA Astrophysics Data System (ADS)

Garza, Alejandro J.; Bulik, Ireneusz W.; Alencar, Ana G. Sousa; Sun, Jianwei; Perdew, John P.; Scuseria, Gustavo E.

2016-04-01

Contrary to standard coupled cluster doubles (CCD) and Brueckner doubles (BD), singlet-paired analogues of CCD and BD (denoted here as CCD0 and BD0) do not break down when static correlation is present, but neglect substantial amounts of dynamic correlation. In fact, CCD0 and BD0 do not account for any contributions from multielectron excitations involving only same-spin electrons at all. We exploit this feature to add - without introducing double counting, self-interaction, or increase in cost - the missing correlation to these methods via meta-GGA (generalised gradient approximation) density functionals (Tao-Perdew-Staroverov-Scuseria and strongly constrained and appropriately normed). Furthermore, we improve upon these CCD0+DFT blends by invoking range separation: the short- and long-range correlations absent in CCD0/BD0 are evaluated with density functional theory and the direct random phase approximation, respectively. This corrects the description of long-range van der Waals forces. Comprehensive benchmarking shows that the combinations presented here are very accurate for weakly correlated systems, while also providing a reasonable description of strongly correlated problems without resorting to symmetry breaking.

Adolescent-onset alcohol abuse exacerbates the influence of childhood conduct disorder on late adolescent and early adult antisocial behaviour

PubMed Central

Howard, Richard; Finn, Peter; Jose, Paul; Gallagher, Jennifer

2012-01-01

This study tested the hypothesis that adolescent-onset alcohol abuse (AOAA) would both mediate and moderate the effect of childhood conduct disorder on antisocial behaviour in late adolescence and early adulthood. A sample comprising 504 young men and women strategically recruited from the community were grouped using the criteria of the Diagnostic and Statistical Manual (DSM-IV, American Psychiatric Association. (1994). Diagnostic and statistical manual of mental disorders (4th ed.). Washington, DC: APA), as follows: neither childhood conduct disorder (CCD) nor alcohol abuse/dependence; CCD but no alcohol abuse or dependence; alcohol abuse/dependence but no CCD; both CCD and alcohol abuse/dependence. The outcome measure was the sum of positive responses to 55 interview items capturing a variety of antisocial behaviours engaged in since age 15. Severity of lifetime alcohol-related and CCD problems served as predictor variables in regression analysis. Antisocial behaviour problems were greatest in individuals with a history of co-occurring conduct disorder (CD) and alcohol abuse/dependence. While CCD was strongly predictive of adult antisocial behaviour, this effect was both mediated and moderated (exacerbated) by AOAA. PMID:23459369

Digital X-ray camera for quality evaluation three-dimensional topographic reconstruction of single crystals of biological macromolecules

NASA Technical Reports Server (NTRS)

Borgstahl, Gloria (Inventor); Lovelace, Jeff (Inventor); Snell, Edward Holmes (Inventor); Bellamy, Henry (Inventor)

2008-01-01

The present invention provides a digital topography imaging system for determining the crystalline structure of a biological macromolecule, wherein the system employs a charge coupled device (CCD) camera with antiblooming circuitry to directly convert x-ray signals to electrical signals without the use of phosphor and measures reflection profiles from the x-ray emitting source after x-rays are passed through a sample. Methods for using said system are also provided.

Control and management of congenital Chagas disease in Europe and other non-endemic countries: current policies and practices.

PubMed

Soriano-Arandes, Antoni; Angheben, Andrea; Serre-Delcor, Nuria; Treviño-Maruri, Begoña; Gómez I Prat, Jordi; Jackson, Yves

2016-05-01

Identifying pregnant women infected with Trypanosoma cruzi is one of the major challenges for preventing and controlling Chagas disease (CD) in non-endemic countries. The aim of this paper was to perform a policy evaluation of the current practices of congenital Chagas disease (CCD) control in non-endemic countries and to propose specific targets for enhanced interventions to tackle this emerging health problem outside the endemic areas of Latin America. We conducted a mixed method review of CCD policy strategies by searching the literature in the PubMed, Google Scholar and the World Health Organization (WHO) databases using the key terms 'CCD', 'paediatric Chagas disease' and 'non-endemic countries'; as free text and combined as one phrase to increase the search sensitivity. Reviews, recommendations, guidelines and control/surveillance programme reports were included. Of 427 CCD papers identified in non-endemic countries, 44 matched the inclusion. Although local programmes were launched in different countries with large numbers of Latin American immigrants, there were considerable disparities in terms of the programmes' distribution, delivery, integration and appropriated CCD control strategies. Moreover, Catalonia, Spain is the only region/country with an established systematic monitoring of CCD in pregnant women from Latin American countries. Given the worldwide dissemination of CD, the nature of its vertical transmission, and the gaps of the current strategies in non-endemic countries, there is an urgent need to standardise, expand and reinforce the control measures against CCD transmission. © 2016 John Wiley & Sons Ltd.

Astro-geodetic platform for high accuracy geoid determinat ion

NASA Astrophysics Data System (ADS)

Bǎdescu, Octavian; Nedelcu, Dan Alin; Cǎlin, Alexandru; Dumitru, Paul Daniel; Cǎlin, Lavinia A.; Popescu, Marcel

The paper presents first technical realizations of a mobile platform for vertical deviation determination at a satisfactory precision and low cost. The conception of the platform was made in the framework of a project regarding CCD astro-geodetic vertical deviation for geoid determination or geoid modeling. The project with the acronym A-GEO represents a collaboration between Technical University of Civil Engineering Bucharest - Faculty of Geodesy, (TUCEB-FG), Astronomical Institute of the Romanian academy (AIRA), and a private partner GeoGIS Proiect S.R.L. The paper presents some hardware and software aspects regarding design, development, and automation of the platform, based on an electro-optical geodetic instrument, CCD observations and satellite time synchronization for astro-geodetic measurements.

Pattern-Recognition Processor Using Holographic Photopolymer

NASA Technical Reports Server (NTRS)

Chao, Tien-Hsin; Cammack, Kevin

2006-01-01

proposed joint-transform optical correlator (JTOC) would be capable of operating as a real-time pattern-recognition processor. The key correlation-filter reading/writing medium of this JTOC would be an updateable holographic photopolymer. The high-resolution, high-speed characteristics of this photopolymer would enable pattern-recognition processing to occur at a speed three orders of magnitude greater than that of state-of-the-art digital pattern-recognition processors. There are many potential applications in biometric personal identification (e.g., using images of fingerprints and faces) and nondestructive industrial inspection. In order to appreciate the advantages of the proposed JTOC, it is necessary to understand the principle of operation of a conventional JTOC. In a conventional JTOC (shown in the upper part of the figure), a collimated laser beam passes through two side-by-side spatial light modulators (SLMs). One SLM displays a real-time input image to be recognized. The other SLM displays a reference image from a digital memory. A Fourier-transform lens is placed at its focal distance from the SLM plane, and a charge-coupled device (CCD) image detector is placed at the back focal plane of the lens for use as a square-law recorder. Processing takes place in two stages. In the first stage, the CCD records the interference pattern between the Fourier transforms of the input and reference images, and the pattern is then digitized and saved in a buffer memory. In the second stage, the reference SLM is turned off and the interference pattern is fed back to the input SLM. The interference pattern thus becomes Fourier-transformed, yielding at the CCD an image representing the joint-transform correlation between the input and reference images. This image contains a sharp correlation peak when the input and reference images are matched. The drawbacks of a conventional JTOC are the following: The CCD has low spatial resolution and is not an ideal square-law detector for the purpose of holographic recording of interference fringes. A typical state-of-the-art CCD has a pixel-pitch limited resolution of about 100 lines/mm. In contrast, the holographic photopolymer to be used in the proposed JTOC offers a resolution > 2,000 lines/mm. In addition to being disadvantageous in itself, the low resolution of the CCD causes overlap of a DC term and the desired correlation term in the output image. This overlap severely limits the correlation signal-to-noise ratio. The two-stage nature of the process limits the achievable throughput rate. A further limit is imposed by the low frame rate (typical video rates) of low- and medium-cost commercial CCDs.

The reliability of cytoplasmic CD3 and CD22 antigen expression in the immunodiagnosis of acute leukemia: a study of 500 cases.

PubMed

Janossy, G; Coustan-Smith, E; Campana, D

1989-03-01

Current views about the origin of acute lymphoid leukemia (ALL) emphasize the importance of maturation arrest at a precursor cell level. Recently, the CD22 antigen has been identified in the cytoplasm of normal bone marrow-borne immature B lineage cells, while the CD3 antigen (epsilon chain) has been detected within normal immature thymic blasts. In the first part our study performed on 100 cases of known acute leukemias, the expression of such cytoplasmic molecules, referred to as cCD22 and cCD3, was analyzed together with their appearance in the leukemic cells' membrane (mCD22 and mCD3). The presence of cCD22 in B-lineage ALL and that of cCD3 in T-ALL has indeed fully confirmed the diagnosis reached by other markers, and mCD22 and mCD3 were expressed on only a few cases of B- and T-lineage ALL, also revealing a degree of developmental asynchrony within leukemic blasts. In the subsequent analysis both cCD22 and cCD3 have been included in a standard panel of diagnostic reagents applied on 500 consecutive cases of acute leukemia. Here the aim was to analyze both the diagnostic precision of individual markers and the heterogeneity of various leukemic types in terms of the expression of membrane and intracellular antigens and their cytochemical features (Sudan Black B and esterases). It has been found that cCD22 and cCD3 are exquisitely specific for B-precursor ALL (TdT+, CD19+) and T-ALL (TdT+, CD7+), respectively, while both markers are absent in acute myeloblastic leukemia (AML) and acute myelomonocytic and monocytic leukemia (AMML/AMoL). These observations contrast the findings which demonstrate that 31% of cases among nonlymphoid acute leukemia (including AML and AMML) express CD7 and/or TdT. The study of myeloid antigens detected by CD13, CD33, and CD14 is also informative and complementary, both in diagnosing and subdividing the AML and AMML/AMoL groups. The peculiar main observation of this study is that only with the combined use of these markers in a microplate assay for membrane antigens, followed by double staining for intracellular antigens such as terminal deoxynucleotidyl transferase, cCD3, cCD22, c mu heavy chain, and T cell receptor beta, it is possible to safely establish the lineage affiliation and subgrouping of virtually all acute leukemias. Among these cases are those with aberrant combinations of markers, including 14% of B-lineage ALL (cCD22+,CD19+,TdT+) and a single case T-ALL (cCD3+,CD7+,TdT+), which exhibit CD13 and/or CD33 antigens, cases with mixtures of ALL and AML blasts, and 1.2% of acute leukemias which lack lineage affiliation and can be regarded as acute undifferentiated leukemia.

Visualization of hump formation in high-speed gas metal arc welding

NASA Astrophysics Data System (ADS)

Wu, C. S.; Zhong, L. M.; Gao, J. Q.

2009-11-01

The hump bead is a typical weld defect observed in high-speed welding. Its occurrence limits the improvement of welding productivity. Visualization of hump formation during high-speed gas metal arc welding (GMAW) is helpful in the better understanding of the humping phenomena so that effective measures can be taken to suppress or decrease the tendency of hump formation and achieve higher productivity welding. In this study, an experimental system was developed to implement vision-based observation of the weld pool behavior during high-speed GMAW. Considering the weld pool characteristics in high-speed welding, a narrow band-pass and neutral density filter was equipped for the CCD camera, the suitable exposure time was selected and side view orientation of the CCD camera was employed. The events that took place at the rear portion of the weld pools were imaged during the welding processes with and without hump bead formation, respectively. It was found that the variation of the weld pool surface height and the solid-liquid interface at the pool trailing with time shows some useful information to judge whether the humping phenomenon occurs or not.

Hyper Suprime-Cam: characteristics of 116 fully depleted back-illuminated CCDs

NASA Astrophysics Data System (ADS)

Kamata, Yukiko; Miyazaki, Satoshi; Nakaya, Hidehiko; Komiyama, Yutaka; Obuchi, Yoshiyuki; Kawanomoto, Satoshi; Uraguchi, Fumihiro; Utsumi, Yosuke; Suzuki, Hisanori; Miyazaki, Yasuhito; Muramatsu, Masaharu

2012-07-01

Hyper Suprime-Cam (HSC)1,2 is a wide field imaging camera with the field of view (FOV) 1.5 degree diameter, which is to be installed at the prime focus of the Subaru Telescope. The large FOV is realized by the 116 2K × 4K pixels fully depleted back-illuminated CCD (FDCCD) with 15 μm pixel square. The acceptance inspection of the CCDs started around the end of 2009 and finished June 2011. We measured basic characteristics such as charge transfer efficiency (CTE), dark current, readout noise, linearity and the number of the dead column for all CCDs, and measured the quantum effciency (QE) of 21 CCDs. As a result, we confirmed exceptional quality and performance fdor all CCDs ans were able to select the best pissible 116 CCDs. We also measured the flatness of each CCD at room temperature, and optimally placed them on the focal plane plate. In this paper, we report the results of the acceptance inspection asn the installation process into the HSC dewar3,4.

Numerically correcting the joint misplacement of the sub-holograms in spatial synthetic aperture digital Fresnel holography.

PubMed

Jiang, Hongzhen; Zhao, Jianlin; Di, Jianglei; Qin, Chuan

2009-10-12

We propose an effective reconstruction method for correcting the joint misplacement of the sub-holograms caused by the displacement error of CCD in spatial synthetic aperture digital Fresnel holography. For every two adjacent sub-holograms along the motion path of CCD, we reconstruct the corresponding holographic images under different joint distances between the sub-holograms and then find out the accurate joint distance by evaluating the quality of the corresponding synthetic reconstructed images. Then the accurate relative position relationships of the sub-holograms can be confirmed according to all of the identified joint distances, with which the accurate synthetic reconstructed image can be obtained by superposing the reconstruction results of the sub-holograms. The numerical reconstruction results are in agreement with the theoretical analysis. Compared with the traditional reconstruction method, this method could be used to not only correct the joint misplacement of the sub-holograms without the limitation of the actually overlapping circumstances of the adjacent sub-holograms, but also make the joint precision of the sub-holograms reach sub-pixel accuracy.

New technology and techniques for x-ray mirror calibration at PANTER

NASA Astrophysics Data System (ADS)

Freyberg, Michael J.; Budau, Bernd; Burkert, Wolfgang; Friedrich, Peter; Hartner, Gisela; Misaki, Kazutami; Mühlegger, Martin

2008-07-01

The PANTER X-ray Test Facility has been utilized successfully for developing and calibrating X-ray astronomical instrumentation for observatories such as ROSAT, Chandra, XMM-Newton, Swift, etc. Future missions like eROSITA, SIMBOL-X, or XEUS require improved spatial resolution and broader energy band pass, both for optics and for cameras. Calibration campaigns at PANTER have made use of flight spare instrumentation for space applications; here we report on a new dedicated CCD camera for on-ground calibration, called TRoPIC. As the CCD is similar to ones used for eROSITA (pn-type, back-illuminated, 75 μm pixel size, frame store mode, 450 μm micron wafer thickness, etc.) it can serve as prototype for eROSITA camera development. New techniques enable and enhance the analysis of measurements of eROSITA shells or silicon pore optics. Specifically, we show how sub-pixel resolution can be utilized to improve spatial resolution and subsequently the characterization of of mirror shell quality and of point spread function parameters in particular, also relevant for position reconstruction of astronomical sources in orbit.

Postirradiation behavior of p-channel charge-coupled devices irradiated at 153 K

NASA Astrophysics Data System (ADS)

Gow, Jason P. D.; Wood, Daniel; Murray, Neil J.; Burt, David; Hall, David J.; Dryer, Ben; Holland, Andrew D.

2016-04-01

The displacement damage hardness that can be achieved using p-channel charge-coupled devices (CCD) was originally demonstrated in 1997, and since then a number of other studies have demonstrated an improved tolerance to radiation-induced charge transfer inefficiency when compared to n-channel CCDs. A number of recent studies have also shown that the temperature history of the device after the irradiation impacts the performance of the detector, linked to the mobility of defects at different temperatures. The initial results from an e2v technologies p-channel CCD204 irradiated at 153 K with 10-MeV equivalent proton fluences of 1.24×109 and 1.24×1011 protons cm-2 is described. The dark current, cosmetic quality, and the number of defects identified using trap pumping immediately were monitored after the irradiation for a period of 150 h with the device held at 153 K and then after different periods of time at room temperature. The device also exhibited a flatband voltage shift of around 30 mV/krad, determined by the reduction in full well capacity.

NASA Imaging for Safety, Science, and History

NASA Technical Reports Server (NTRS)

Grubbs, Rodney; Lindblom, Walt; Bowerman, Deborah S. (Technical Monitor)

2002-01-01

Since its creation in 1958 NASA has been making and documenting history, both on Earth and in space. To complete its missions NASA has long relied on still and motion imagery to document spacecraft performance, see what can't be seen by the naked eye, and enhance the safety of astronauts and expensive equipment. Today, NASA is working to take advantage of new digital imagery technologies and techniques to make its missions more safe and efficient. An HDTV camera was on-board the International Space Station from early August, to mid-December, 2001. HDTV cameras previously flown have had degradation in the CCD during the short duration of a Space Shuttle flight. Initial performance assessment of the CCD during the first-ever long duration space flight of a HDTV camera and earlier flights is discussed. Recent Space Shuttle launches have been documented with HDTV cameras and new long lenses giving clarity never before seen with video. Examples and comparisons will be illustrated between HD, highspeed film, and analog video of these launches and other NASA tests. Other uses of HDTV where image quality is of crucial importance will also be featured.

Optical Observation, Image-processing, and Detection of Space Debris in Geosynchronous Earth Orbit

NASA Astrophysics Data System (ADS)

Oda, H.; Yanagisawa, T.; Kurosaki, H.; Tagawa, M.

2014-09-01

We report on optical observations and an efficient detection method of space debris in the geosynchronous Earth orbit (GEO). We operate our new Australia Remote Observatory (ARO) where an 18 cm optical telescope with a charged-coupled device (CCD) camera covering a 3.14-degree field of view is used for GEO debris survey, and analyse datasets of successive CCD images using the line detection method (Yanagisawa and Nakajima 2005). In our operation, the exposure time of each CCD image is set to be 3 seconds (or 5 seconds), and the time interval of CCD shutter open is about 4.7 seconds (or 6.7 seconds). In the line detection method, a sufficient number of sample objects are taken from each image based on their shape and intensity, which includes not only faint signals but also background noise (we take 500 sample objects from each image in this paper). Then we search a sequence of sample objects aligning in a straight line in the successive images to exclude the noise sample. We succeed in detecting faint signals (down to about 1.8 sigma of background noise) by applying the line detection method to 18 CCD images. As a result, we detected about 300 GEO objects up to magnitude of 15.5 among 5 nights data. We also calculate orbits of objects detected using the Simplified General Perturbations Satellite Orbit Model 4(SGP4), and identify the objects listed in the two-line-element (TLE) data catalogue publicly provided by the U.S. Strategic Command (USSTRATCOM). We found that a certain amount of our detections are new objects that are not contained in the catalogue. We conclude that our ARO and detection method posse a high efficiency detection of GEO objects despite the use of comparatively-inexpensive observation and analysis system. We also describe the image-processing specialized for the detection of GEO objects (not for usual astronomical objects like stars) in this paper.

An ultrahigh-speed color video camera operating at 1,000,000 fps with 288 frame memories

NASA Astrophysics Data System (ADS)

Kitamura, K.; Arai, T.; Yonai, J.; Hayashida, T.; Kurita, T.; Maruyama, H.; Namiki, J.; Yanagi, T.; Yoshida, T.; van Kuijk, H.; Bosiers, Jan T.; Saita, A.; Kanayama, S.; Hatade, K.; Kitagawa, S.; Etoh, T. Goji

2008-11-01

We developed an ultrahigh-speed color video camera that operates at 1,000,000 fps (frames per second) and had capacity to store 288 frame memories. In 2005, we developed an ultrahigh-speed, high-sensitivity portable color camera with a 300,000-pixel single CCD (ISIS-V4: In-situ Storage Image Sensor, Version 4). Its ultrahigh-speed shooting capability of 1,000,000 fps was made possible by directly connecting CCD storages, which record video images, to the photodiodes of individual pixels. The number of consecutive frames was 144. However, longer capture times were demanded when the camera was used during imaging experiments and for some television programs. To increase ultrahigh-speed capture times, we used a beam splitter and two ultrahigh-speed 300,000-pixel CCDs. The beam splitter was placed behind the pick up lens. One CCD was located at each of the two outputs of the beam splitter. The CCD driving unit was developed to separately drive two CCDs, and the recording period of the two CCDs was sequentially switched. This increased the recording capacity to 288 images, an increase of a factor of two over that of conventional ultrahigh-speed camera. A problem with the camera was that the incident light on each CCD was reduced by a factor of two by using the beam splitter. To improve the light sensitivity, we developed a microlens array for use with the ultrahigh-speed CCDs. We simulated the operation of the microlens array in order to optimize its shape and then fabricated it using stamping technology. Using this microlens increased the light sensitivity of the CCDs by an approximate factor of two. By using a beam splitter in conjunction with the microlens array, it was possible to make an ultrahigh-speed color video camera that has 288 frame memories but without decreasing the camera's light sensitivity.

Flat-panel detector, CCD cameras, and electron-beam-tube-based video for use in portal imaging

NASA Astrophysics Data System (ADS)

Roehrig, Hans; Tang, Chuankun; Cheng, Chee-Way; Dallas, William J.

1998-07-01

This paper provides a comparison of some imaging parameters of four portal imaging systems at 6 MV: a flat panel detector, two CCD cameras and an electron beam tube based video camera. Measurements were made of signal and noise and consequently of signal-to-noise per pixel as a function of the exposure. All systems have a linear response with respect to exposure, and with the exception of the electron beam tube based video camera, the noise is proportional to the square-root of the exposure, indicating photon-noise limitation. The flat-panel detector has a signal-to-noise ratio, which is higher than that observed with both CCD-Cameras or with the electron beam tube based video camera. This is expected because most portal imaging systems using optical coupling with a lens exhibit severe quantum-sinks. The measurements of signal-and noise were complemented by images of a Las Vegas-type aluminum contrast detail phantom, located at the ISO-Center. These images were generated at an exposure of 1 MU. The flat-panel detector permits detection of Aluminum holes of 1.2 mm diameter and 1.6 mm depth, indicating the best signal-to-noise ratio. The CCD-cameras rank second and third in signal-to- noise ratio, permitting detection of Aluminum-holes of 1.2 mm diameter and 2.2 mm depth (CCD_1) and of 1.2 mm diameter and 3.2 mm depth (CCD_2) respectively, while the electron beam tube based video camera permits detection of only a hole of 1.2 mm diameter and 4.6 mm depth. Rank Order Filtering was applied to the raw images from the CCD-based systems in order to remove the direct hits. These are camera responses to scattered x-ray photons which interact directly with the CCD of the CCD-Camera and generate 'Salt and Pepper type noise,' which interferes severely with attempts to determine accurate estimates of the image noise. The paper also presents data on the metal-phosphor's photon gain (the number of light-photons per interacting x-ray photon).

Panoramic 3D Reconstruction by Fusing Color Intensity and Laser Range Data

NASA Astrophysics Data System (ADS)

Jiang, Wei; Lu, Jian

Technology for capturing panoramic (360 degrees) three-dimensional information in a real environment have many applications in fields: virtual and complex reality, security, robot navigation, and so forth. In this study, we examine an acquisition device constructed of a regular CCD camera and a 2D laser range scanner, along with a technique for panoramic 3D reconstruction using a data fusion algorithm based on an energy minimization framework. The acquisition device can capture two types of data of a panoramic scene without occlusion between two sensors: a dense spatio-temporal volume from a camera and distance information from a laser scanner. We resample the dense spatio-temporal volume for generating a dense multi-perspective panorama that has equal spatial resolution to that of the original images acquired using a regular camera, and also estimate a dense panoramic depth-map corresponding to the generated reference panorama by extracting trajectories from the dense spatio-temporal volume with a selecting camera. Moreover, for determining distance information robustly, we propose a data fusion algorithm that is embedded into an energy minimization framework that incorporates active depth measurements using a 2D laser range scanner and passive geometry reconstruction from an image sequence obtained using the CCD camera. Thereby, measurement precision and robustness can be improved beyond those available by conventional methods using either passive geometry reconstruction (stereo vision) or a laser range scanner. Experimental results using both synthetic and actual images show that our approach can produce high-quality panoramas and perform accurate 3D reconstruction in a panoramic environment.

NAOMI instrument: a product line of compact and versatile cameras designed for HR and VHR missions in Earth observation

NASA Astrophysics Data System (ADS)

Luquet, Ph.; Brouard, L.; Chinal, E.

2017-11-01

Astrium has developed a product line of compact and versatile instruments for HR and VHR missions in Earth Observation. These cameras consist on a Silicon Carbide Korsch-type telescope, a focal plane with one or several retina modules - including five lines CCD, optical filters and front end electronics - and the instrument main electronics. Several versions have been developed with a telescope pupil diameter from 200 mm up to 650 mm, covering a large range of GSD (from 2.5 m down to sub-metric) and swath (from 10km up to 30 km) and compatible with different types of platform. Nine cameras have already been manufactured for five different programs: ALSAT2 (Algeria), SSOT (Chile), SPOT6 & SPOT7 (France), KRS (Kazakhstan) and VNREDSat (Vietnam). Two of them have already been launched and are delivering high quality images.

The rotationally modulated Zeeman spectrum at nearly 10 to the 9th Gauss of the white dwarf PG 1031 + 234

NASA Technical Reports Server (NTRS)

Latter, William B.; Schmidt, Gary D.; Green, Richard F.

1987-01-01

Detailed analyses are performed of high-quality, phase-resolved CCD spectroscopy of the absorption-line spectrum throughout its rotation period of the new white dwarf PG 1031 + 234. The spectral variations are discussed and compared with new theoretical calculations of the behavior of hydrogen in strong magnetic fields. This analysis is then extended through a modeling procedure which produces a synthetic magnetically distorted spectrum for a star of arbitrary field strength and structure. The results confirm that PG 1031 + 234 possesses the strongest field yet detected on a white dwarf, with regions on the surface spanning the range of about 200 to nearly 1000 MG. The spectroscopic data reflect a field pattern containing a slightly offset global component of polar field strength of about 500 MG together with a localized magnetic 'spot' whose central field approaches 2000 MG.

A high-speed pnCCD detector system for optical applications

NASA Astrophysics Data System (ADS)

Hartmann, R.; Buttler, W.; Gorke, H.; Herrmann, S.; Holl, P.; Meidinger, N.; Soltau, H.; Strüder, L.

2006-11-01

Measurements of a frame-store pnCCD detector system, optimized for high-speed applications in the optical and near infrared (NIR) region, will be presented. The device with an image area of 13.5 mm by 13.5 mm and a pixelsize of 51 μm by 51 μm exhibits a readout time faster than 1100 frames per second with an overall electronic noise contribution of less than three electrons. Variable operation modes of the detector system allow for even higher readout speeds by a pixel binning in transfer direction or, at slightly slower readout speeds, a further improvement in noise performance. We will also present the concept of a data acquisition system being able to handle pixel rates of more than 75 megapixel per second. The application of an anti-reflective coating on the ultra-thin entrance window of the back illuminated detector together with the large sensitive volume ensures a high and uniform detection efficiency from the ultra violet to the NIR.

An Experimental Study of the Structure of Turbulent Non-Premixed Jet Flames in Microgravity

NASA Astrophysics Data System (ADS)

Boxx, Isaac; Idicheria, Cherian; Clemens, Noel

2000-11-01

The aim of this work is to investigate the structure of transitional and turbulent non-premixed jet flames under microgravity conditions. The microgravity experiments are being conducted using a newly developed drop rig and the University of Texas 1.5 second drop tower. The rig itself measures 16”x33”x38” and contains a co-flowing round jet flame facility, flow control system, CCD camera, and data/image acquisition computer. These experiments are the first phase of a larger study being conducted at the NASA Glenn Research Center 2.2 second drop tower facility. The flames being studied include methane and propane round jet flames at jet exit Reynolds numbers as high as 10,000. The primary diagnostic technique employed is emission imaging of flame luminosity using a relatively high-speed (350 fps) CCD camera. The high-speed images are used to study flame height, flame tip dynamics and burnout characteristics. Results are compared to normal gravity experimental results obtained in the same apparatus.

Multiport backside-illuminated CCD imagers for high-frame-rate camera applications

NASA Astrophysics Data System (ADS)

Levine, Peter A.; Sauer, Donald J.; Hseuh, Fu-Lung; Shallcross, Frank V.; Taylor, Gordon C.; Meray, Grazyna M.; Tower, John R.; Harrison, Lorna J.; Lawler, William B.

1994-05-01

Two multiport, second-generation CCD imager designs have been fabricated and successfully tested. They are a 16-port 512 X 512 array and a 32-port 1024 X 1024 array. Both designs are back illuminated, have on-chip CDS, lateral blooming control, and use a split vertical frame transfer architecture with full frame storage. The 512 X 512 device has been operated at rates over 800 frames per second. The 1024 X 1024 device has been operated at rates over 300 frames per second. The major changes incorporated in the second-generation design are, reduction in gate length in the output area to give improved high-clock-rate performance, modified on-chip CDS circuitry for reduced noise, and optimized implants to improve performance of blooming control at lower clock amplitude. This paper discusses the imager design improvements and presents measured performance results at high and moderate frame rates. The design and performance of three moderate frame rate cameras are discussed.

100-kHz shot-to-shot broadband data acquisition for high-repetition-rate pump-probe spectroscopy.

PubMed

Kanal, Florian; Keiber, Sabine; Eck, Reiner; Brixner, Tobias

2014-07-14

Shot-to-shot broadband detection is common in ultrafast pump-probe spectroscopy. Taking advantage of the intensity correlation of subsequent laser pulses improves the signal-to-noise ratio. Finite data readout times of CCD chips in the employed spectrometer and the maximum available speed of mechanical pump-beam choppers typically limit this approach to lasers with repetition rates of a few kHz. For high-repetition (≥ 100 kHz) systems, one typically averages over a larger number of laser shots leading to inferior signal-to-noise ratios or longer measurement times. Here we demonstrate broadband shot-to-shot detection in transient absorption spectroscopy with a 100-kHz femtosecond laser system. This is made possible using a home-built high-speed chopper with external laser synchronization and a fast CCD line camera. Shot-to-shot detection can reduce the data acquisition time by two orders of magnitude compared to few-kHz lasers while keeping the same signal-to-noise ratio.

Measuring the effective pixel positions for the HARPS3 CCD

NASA Astrophysics Data System (ADS)

Hall, Richard D.; Thompson, Samantha; Queloz, Didier

2016-07-01

We present preliminary results from an experiment designed to measure the effective pixel positions of a CCD to sub-pixel precision. This technique will be used to characterise the 4k x 4k CCD destined for the HARPS-3 spectrograph. The principle of coherent beam interference is used to create intensity fringes along one axis of the CCD. By sweeping the physical parameters of the experiment, the geometry of the fringes can be altered which is used to probe the pixel structure. We also present the limitations of the current experimental set-up and suggest what will be implemented in the future to vastly improve the precision of the measurements.

64 x 64 thresholding photodetector array for optical pattern recognition

NASA Astrophysics Data System (ADS)

Langenbacher, Harry; Chao, Tien-Hsin; Shaw, Timothy; Yu, Jeffrey W.

1993-10-01

A high performance 32 X 32 peak detector array is introduced. This detector consists of a 32 X 32 array of thresholding photo-transistor cells, manufactured with a standard MOSIS digital 2-micron CMOS process. A built-in thresholding function that is able to perform 1024 thresholding operations in parallel strongly distinguishes this chip from available CCD detectors. This high speed detector offers responses from one to 10 milliseconds that is much higher than the commercially available CCD detectors operating at a TV frame rate. The parallel multiple peaks thresholding detection capability makes it particularly suitable for optical correlator and optoelectronically implemented neural networks. The principle of operation, circuit design and the performance characteristics are described. Experimental demonstration of correlation peak detection is also provided. Recently, we have also designed and built an advanced version of a 64 X 64 thresholding photodetector array chip. Experimental investigation of using this chip for pattern recognition is ongoing.

A TV Camera System Which Extracts Feature Points For Non-Contact Eye Movement Detection

NASA Astrophysics Data System (ADS)

Tomono, Akira; Iida, Muneo; Kobayashi, Yukio

1990-04-01

This paper proposes a highly efficient camera system which extracts, irrespective of background, feature points such as the pupil, corneal reflection image and dot-marks pasted on a human face in order to detect human eye movement by image processing. Two eye movement detection methods are sugested: One utilizing face orientation as well as pupil position, The other utilizing pupil and corneal reflection images. A method of extracting these feature points using LEDs as illumination devices and a new TV camera system designed to record eye movement are proposed. Two kinds of infra-red LEDs are used. These LEDs are set up a short distance apart and emit polarized light of different wavelengths. One light source beams from near the optical axis of the lens and the other is some distance from the optical axis. The LEDs are operated in synchronization with the camera. The camera includes 3 CCD image pick-up sensors and a prism system with 2 boundary layers. Incident rays are separated into 2 wavelengths by the first boundary layer of the prism. One set of rays forms an image on CCD-3. The other set is split by the half-mirror layer of the prism and forms an image including the regularly reflected component by placing a polarizing filter in front of CCD-1 or another image not including the component by not placing a polarizing filter in front of CCD-2. Thus, three images with different reflection characteristics are obtained by three CCDs. Through the experiment, it is shown that two kinds of subtraction operations between the three images output from CCDs accentuate three kinds of feature points: the pupil and corneal reflection images and the dot-marks. Since the S/N ratio of the subtracted image is extremely high, the thresholding process is simple and allows reducting the intensity of the infra-red illumination. A high speed image processing apparatus using this camera system is decribed. Realtime processing of the subtraction, thresholding and gravity position calculation of the feature points is possible.

Ontogeny of flow-stimulated potassium secretion in rabbit cortical collecting duct: functional and molecular aspects.

PubMed

Woda, Craig B; Miyawaki, Nobuyuki; Ramalakshmi, Santhanam; Ramkumar, Mohan; Rojas, Raul; Zavilowitz, Beth; Kleyman, Thomas R; Satlin, Lisa M

2003-10-01

High urinary flow rates stimulate K secretion in the fully differentiated but not neonatal or weanling rabbit cortical collecting duct (CCD). Both small-conductance secretory K and high-conductance Ca2+/stretch-activated maxi-K channels have been identified in the apical membrane of the mature CCD by patch-clamp analysis. We reported that flow-stimulated net K secretion in the adult rabbit CCD is 1) blocked by TEA and charybdotoxin, inhibitors of intermediate- and high-conductance (maxi-K) Ca2+-activated K channels, and 2) associated with increases in net Na absorption and intracellular Ca2+ concentration ([Ca2+]i). The present study examined whether the absence of flow-stimulated K secretion early in life is due to a 1) limited flow-induced rise in net Na absorption and/or [Ca2+]i and/or 2) paucity of apical maxi-K channels. An approximately sixfold increase in tubular fluid flow rate in CCDs isolated from 4-wk-old rabbits and microperfused in vitro led to an increase in net Na absorption and [Ca2+]i, similar in magnitude to the response observed in 6-wk-old tubules, but it failed to generate an increase in net K secretion. By 5 wk of age, there was a small, but significant, flow-stimulated rise in net K secretion that increased further by 6 wk of life. Luminal perfusion with iberiotoxin blocked the flow stimulation of net K secretion in the adult CCD, confirming the identity of the maxi-K channel in this response. Maxi-K channel alpha-subunit message was consistently detected in single CCDs from animals >/=4 wk of age by RT-PCR. Indirect immunofluorescence microscopy using antibodies directed against the alpha-subunit revealed apical labeling of intercalated cells in cryosections from animals >/=5 wk of age; principal cell labeling was generally intracellular and punctate. We speculate that the postnatal appearance of flow-dependent K secretion is determined by the transcriptional/translational regulation of expression of maxi-K channels. Furthermore, our studies suggest a novel function for intercalated cells in mediating flow-stimulated K secretion.

Back-illuminate fiber system research for multi-object fiber spectroscopic telescope

NASA Astrophysics Data System (ADS)

Zhou, Zengxiang; Liu, Zhigang; Hu, Hongzhuan; Wang, Jianping; Zhai, Chao; Chu, Jiaru

2016-07-01

In the telescope observation, the position of fiber will highly influence the spectra efficient input in the fiber to the spectrograph. When the fibers were back illuminated on the spectra end, they would export light on the positioner end, so the CCD cameras could capture the photo of fiber tip position covered the focal plane, calculates the precise position information by light centroid method and feeds back to control system. A set of fiber back illuminated system was developed which combined to the low revolution spectro instruments in LAMOST. It could provide uniform light output to the fibers, meet the requirements for the CCD camera measurement. The paper was introduced the back illuminated system design and different test for the light resource. After optimization, the effect illuminated system could compare with the integrating sphere, meet the conditions of fiber position measurement.Using parallel controlled fiber positioner as the spectroscopic receiver is an efficiency observation system for spectra survey, has been used in LAMOST recently, and will be proposed in CFHT and rebuilt telescope Mayall. In the telescope observation, the position of fiber will highly influence the spectra efficient input in the fiber to the spectrograph. When the fibers were back illuminated on the spectra end, they would export light on the positioner end, so the CCD cameras could capture the photo of fiber tip position covered the focal plane, calculates the precise position information by light centroid method and feeds back to control system. After many years on these research, the back illuminated fiber measurement was the best method to acquire the precision position of fibers. In LAMOST, a set of fiber back illuminated system was developed which combined to the low revolution spectro instruments in LAMOST. It could provide uniform light output to the fibers, meet the requirements for the CCD camera measurement and was controlled by high-level observation system which could shut down during the telescope observation. The paper was introduced the back illuminated system design and different test for the light resource. After optimization, the effect illuminated system could compare the integrating sphere, meet the conditions of fiber position measurement.

Enabling Joint Commission Medication Reconciliation Objectives with the HL7 / ASTM Continuity of Care Document Standard

PubMed Central

Dolin, Robert H.; Giannone, Gay; Schadow, Gunther

2007-01-01

We sought to determine how well the HL7 / ASTM Continuity of Care Document (CCD) standard supports the requirements underlying the Joint Commission medication reconciliation recommendations. In particular, the Joint Commission emphasizes that transition points in the continuum of care are vulnerable to communication breakdowns, and that these breakdowns are a common source of medication errors. These transition points are the focus of communication standards, suggesting that CCD can support and enable medication related patient safety initiatives. Data elements needed to support the Joint Commission recommendations were identified and mapped to CCD, and a detailed clinical scenario was constructed. The mapping identified minor gaps, and identified fields present in CCD not specifically identified by Joint Commission, but useful nonetheless when managing medications across transitions of care, suggesting that a closer collaboration between the Joint Commission and standards organizations will be mutually beneficial. The nationally recognized CCD specification provides a standards-based solution for enabling Joint Commission medication reconciliation objectives. PMID:18693823

Enabling joint commission medication reconciliation objectives with the HL7 / ASTM Continuity of Care Document standard.

PubMed

Dolin, Robert H; Giannone, Gay; Schadow, Gunther

2007-10-11

We sought to determine how well the HL7/ASTM Continuity of Care Document (CCD) standard supports the requirements underlying the Joint Commission medication reconciliation recommendations. In particular, the Joint Commission emphasizes that transition points in the continuum of care are vulnerable to communication breakdowns, and that these breakdowns are a common source of medication errors. These transition points are the focus of communication standards, suggesting that CCD can support and enable medication related patient safety initiatives. Data elements needed to support the Joint Commission recommendations were identified and mapped to CCD, and a detailed clinical scenario was constructed. The mapping identified minor gaps, and identified fields present in CCD not specifically identified by Joint Commission, but useful nonetheless when managing medications across transitions of care, suggesting that a closer collaboration between the Joint Commission and standards organizations will be mutually beneficial. The nationally recognized CCD specification provides a standards-based solution for enabling Joint Commission medication reconciliation objectives.

Single-Electron and Single-Photon Sensitivity with a Silicon Skipper CCD

DOE PAGES

Tiffenberg, Javier; Sofo-Haro, Miguel; Drlica-Wagner, Alex; ...

2017-09-26

Here, we have developed ultralow-noise electronics in combination with repetitive, nondestructive readout of a thick, fully depleted charge-coupled device (CCD) to achieve an unprecedented noise level of 0.068 e - rms/pixel. This is the first time that discrete subelectron readout noise has been achieved reproducible over millions of pixels on a stable, large-area detector. This enables the contemporaneous, discrete, and quantized measurement of charge in pixels, irrespective of whether they contain zero electrons or thousands of electrons. Thus, the resulting CCD detector is an ultra-sensitive calorimeter. It is also capable of counting single photons in the optical and near-infrared regime.more » Implementing this innovative non-destructive readout system has a negligible impact on CCD design and fabrication, and there are nearly immediate scientific applications. As a particle detector, this CCD will have unprecedented sensitivity to low-mass dark matter particles and coherent neutrino-nucleus scattering, while future astronomical applications may include direct imaging and spectroscopy of exoplanets.« less

Perceptions of predisposing, enabling, and reinforcing factors influencing the use of a continuity of care document in special needs PLWH.

PubMed

Odlum, Michelle; Gordon, Peter; Camhi, Eli; Schnall, Rebecca; Teixeira, Paul; Bakken, Suzanne

2012-11-01

The provision of personal health information through electronic personal health management tools (EPHMT) has the potential to improve health outcomes. However, little is known about factors that affect EPHMT use in special needs people living with HIV/AIDS (PLWH). The purpose of this study was to describe PLWH perceptions of predisposing, enabling, and reinforcing factors affecting use of one type of EPHMT, a continuity of care document (CCD). Data were collected through focus groups and qualitative interviews with PLWH (n=35), health care providers (N=19) and care site managers (N=5). All participant types identified predisposing barriers to CCD use including literacy, privacy, and technology access and enabling factors to promote CCD use including user support and password simplicity. People living with HIV/AIDS also identified reinforcing factors for recurring CCD use including emergent situations and reducing test redundancy. Results inform methods to promote use of the CCD and other EPHMT by special needs PLWH.

CCD Measurements of Double and Multiple Stars at NAO Rozhen and ASV in 2015

NASA Astrophysics Data System (ADS)

Cvetković, Z.; Pavlović, R.; Boeva, S.

2017-04-01

Results of CCD observations of 154 double or multiple stars, made with the 2 m telescope of the Bulgarian National Astronomical Observatory at Rozhen over five nights in 2015, are presented. This is the ninth series of measurements of CCD frames obtained at Rozhen. We also present results of CCD observations of 323 double or multiple stars made with the 0.6 m telescope of the Serbian Astronomical Station on the mountain of Vidojevica over 23 nights in 2015. This is the fourth series of measurements of CCD frames obtained at this station. This paper contains the results for the position angle and angular separation for 801 pairs and residuals for 127 pairs with published orbital elements or linear solutions. The angular separations are in the range from 1.″52 to 201.″56, with a median angular separation of 8.″26. We also present eight pairs that are measured for the first time and linear elements for five pairs.

Single-Electron and Single-Photon Sensitivity with a Silicon Skipper CCD

NASA Astrophysics Data System (ADS)

Tiffenberg, Javier; Sofo-Haro, Miguel; Drlica-Wagner, Alex; Essig, Rouven; Guardincerri, Yann; Holland, Steve; Volansky, Tomer; Yu, Tien-Tien

2017-09-01

We have developed ultralow-noise electronics in combination with repetitive, nondestructive readout of a thick, fully depleted charge-coupled device (CCD) to achieve an unprecedented noise level of 0.068 e- rms /pixel . This is the first time that discrete subelectron readout noise has been achieved reproducible over millions of pixels on a stable, large-area detector. This enables the contemporaneous, discrete, and quantized measurement of charge in pixels, irrespective of whether they contain zero electrons or thousands of electrons. Thus, the resulting CCD detector is an ultra-sensitive calorimeter. It is also capable of counting single photons in the optical and near-infrared regime. Implementing this innovative non-destructive readout system has a negligible impact on CCD design and fabrication, and there are nearly immediate scientific applications. As a particle detector, this CCD will have unprecedented sensitivity to low-mass dark matter particles and coherent neutrino-nucleus scattering, while future astronomical applications may include direct imaging and spectroscopy of exoplanets.

Single-Electron and Single-Photon Sensitivity with a Silicon Skipper CCD.

PubMed

Tiffenberg, Javier; Sofo-Haro, Miguel; Drlica-Wagner, Alex; Essig, Rouven; Guardincerri, Yann; Holland, Steve; Volansky, Tomer; Yu, Tien-Tien

2017-09-29

We have developed ultralow-noise electronics in combination with repetitive, nondestructive readout of a thick, fully depleted charge-coupled device (CCD) to achieve an unprecedented noise level of 0.068  e^{-} rms/pixel. This is the first time that discrete subelectron readout noise has been achieved reproducible over millions of pixels on a stable, large-area detector. This enables the contemporaneous, discrete, and quantized measurement of charge in pixels, irrespective of whether they contain zero electrons or thousands of electrons. Thus, the resulting CCD detector is an ultra-sensitive calorimeter. It is also capable of counting single photons in the optical and near-infrared regime. Implementing this innovative non-destructive readout system has a negligible impact on CCD design and fabrication, and there are nearly immediate scientific applications. As a particle detector, this CCD will have unprecedented sensitivity to low-mass dark matter particles and coherent neutrino-nucleus scattering, while future astronomical applications may include direct imaging and spectroscopy of exoplanets.

VizieR Online Data Catalog: IC 361 Vilnius photometry (Zdanavicius+, 2010)

NASA Astrophysics Data System (ADS)

Zdanavicius, J.; Bartasiute, S.; Boyle, R. P.; Vrba, F. J.; Zdanavicius, K.

2015-03-01

CCD observations in seven filters U,P,X,Y,Z,V,S of the Vilnius system plus the filter I of the Cousins system were carried out in December of 1999 with a 2K CCD camera on the 1m telescope of the USNO Flagstaff Station (Arizona), which gives a field of the diameter of 20'. Repeated observations in the Vilnius filters were done with the same telescope and a new 2Kx2K CCD camera in March of 2009. During the latter run we have obtained well-calibrated CCD data only for filters Y, Z, V, S, since observations through the remaining three filters on the succeeding night were curtailed by cirrus clouds. Additional frames in the Vilnius filters U,Y,V were taken for the central part of the field (12'x12') in December of 2008 with a 4K CCD camera on the 1.8m Vatican Advanced Technology Telescope (VATT) on Mt. Graham (Arizona). (1 data file).

Design and fabrication of an angle-scanning based platform for the construction of surface plasmon resonance biosensor

NASA Astrophysics Data System (ADS)

Hu, Jiandong; Cao, Baiqiong; Wang, Shun; Li, Jianwei; Wei, Wensong; Zhao, Yuanyuan; Hu, Xinran; Zhu, Juanhua; Jiang, Min; Sun, Xiaohui; Chen, Ruipeng; Ma, Liuzheng

2016-03-01

A sensing system for an angle-scanning optical surface-plasmon-resonance (SPR) based biosensor has been designed with a laser line generator in which a P polarizer is embedded to utilize as an excitation source for producing the surface plasmon wave. In this system, the emitting beam from the laser line generator is controlled to realize the angle-scanning using a variable speed direct current (DC) motor. The light beam reflected from the prism deposited with a 50 nm Au film is then captured using the area CCD array which was controlled by a personal computer (PC) via a universal serial bus (USB) interface. The photoelectric signals from the high speed digital camera (an area CCD array) were converted by a 16 bit A/D converter before it transferred to the PC. One of the advantages of this SPR biosensing platform is greatly demonstrated by the label-free and real-time bio-molecular analysis without moving the area CCD array by following the laser line generator. It also could provide a low-cost surface plasmon resonance platform to improve the detection range in the measurement of bioanalytes. The SPR curve displayed on the PC screen promptly is formed by the effective data from the image on the area CCD array and the sensing responses of the platform to bulk refractive indices were calibrated using various concentrations of ethanol solution. These ethanol concentrations indicated with volumetric fraction of 5%, 10%, 15%, 20%, and 25%, respectively, were experimented to validate the performance of the angle-scanning optic SPR biosensing platform. As a result, the SPR sensor was capable to detect a change in the refractive index of the ethanol solution with the relative high linearity at the correlation coefficient of 0.9842. This greatly enhanced detection range is obtained from the position relationship between the laser line generator and the right-angle prism to allow direct quantification of the samples over a wide range of concentrations.

Deep-UV-sensitive high-frame-rate backside-illuminated CCD camera developments

NASA Astrophysics Data System (ADS)

Dawson, Robin M.; Andreas, Robert; Andrews, James T.; Bhaskaran, Mahalingham; Farkas, Robert; Furst, David; Gershstein, Sergey; Grygon, Mark S.; Levine, Peter A.; Meray, Grazyna M.; O'Neal, Michael; Perna, Steve N.; Proefrock, Donald; Reale, Michael; Soydan, Ramazan; Sudol, Thomas M.; Swain, Pradyumna K.; Tower, John R.; Zanzucchi, Pete

2002-04-01

New applications for ultra-violet imaging are emerging in the fields of drug discovery and industrial inspection. High throughput is critical for these applications where millions of drug combinations are analyzed in secondary screenings or high rate inspection of small feature sizes over large areas is required. Sarnoff demonstrated in1990 a back illuminated, 1024 X 1024, 18 um pixel, split-frame-transfer device running at > 150 frames per second with high sensitivity in the visible spectrum. Sarnoff designed, fabricated and delivered cameras based on these CCDs and is now extending this technology to devices with higher pixel counts and higher frame rates through CCD architectural enhancements. The high sensitivities obtained in the visible spectrum are being pushed into the deep UV to support these new medical and industrial inspection applications. Sarnoff has achieved measured quantum efficiencies > 55% at 193 nm, rising to 65% at 300 nm, and remaining almost constant out to 750 nm. Optimization of the sensitivity is being pursued to tailor the quantum efficiency for particular wavelengths. Characteristics of these high frame rate CCDs and cameras will be described and results will be presented demonstrating high UV sensitivity down to 150 nm.

A novel reflectometer for relative reflectance measurements of CCDs

NASA Astrophysics Data System (ADS)

Hart, Murdock; Barkhouser, Robert H.; Gunn, James E.; Smee, Stephen A.

2016-07-01

The high quantum efficiencies (QE) of backside illuminated charge coupled devices (CCD) has ushered in the age of the large scale astronomical survey. The QE of these devices can be greater than 90%, and is dependent upon the operating temperature, device thickness, backside charging mechanisms, and anti-reflection (AR) coatings. But at optical wavelengths the QE is well approximated as one minus the reflectance, thus the measurement of the backside reflectivity of these devices provides a second independent measure of their QE. We have designed and constructed a novel instrument to measure the relative specular reflectance of CCD detectors, with a significant portion of this device being constructed using a 3D fused deposition model (FDM) printer. This device implements both a monitor and measurement photodiode to simultaneously collect in- cident and reflected measurements reducing errors introduced by the relative reflectance calibration process. While most relative reflectometers are highly dependent upon a precisely repeatable target distance for accurate measurements, we have implemented a method of measurement which minimizes these errors. Using the reflectometer we have measured the reflectance of two types of Hamamatsu CCD detectors. The first device is a Hamamatsu 2k x 4k backside illuminated high resistivity p-type silicon detector which has been optimized to operate in the blue from 380 nm - 650 nm. The second detector being a 2k x 4k backside illuminated high resistivity p-type silicon detector optimized for use in the red from 640 nm - 960 nm. We have not only been able to measure the reflectance of these devices as a function of wavelength we have also sampled the reflectance as a function of position on the device, and found a reflection gradient across these devices.

Colony collapse disorder in Europe.

PubMed

Dainat, Benjamin; Vanengelsdorp, Dennis; Neumann, Peter

2012-02-01

Colony collapse disorder (CCD) is a condition of honey bees, which has contributed in part to the recent major losses of honey bee colonies in the USA. Here we report the first CCD case from outside of the USA. We suggest that more standardization is needed for the case definition to diagnose CCD and to compare data on a global scale. © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.

Typical effects of laser dazzling CCD camera

NASA Astrophysics Data System (ADS)

Zhang, Zhen; Zhang, Jianmin; Shao, Bibo; Cheng, Deyan; Ye, Xisheng; Feng, Guobin

2015-05-01

In this article, an overview of laser dazzling effect to buried channel CCD camera is given. The CCDs are sorted into staring and scanning types. The former includes the frame transfer and interline transfer types. The latter includes linear and time delay integration types. All CCDs must perform four primary tasks in generating an image, which are called charge generation, charge collection, charge transfer and charge measurement. In camera, the lenses are needed to input the optical signal to the CCD sensors, in which the techniques for erasing stray light are used. And the electron circuits are needed to process the output signal of CCD, in which many electronic techniques are used. The dazzling effects are the conjunct result of light distribution distortion and charge distribution distortion, which respectively derive from the lens and the sensor. Strictly speaking, in lens, the light distribution is not distorted. In general, the lens are so well designed and fabricated that its stray light can be neglected. But the laser is of much enough intensity to make its stray light obvious. In CCD image sensors, laser can induce a so large electrons generation. Charges transfer inefficiency and charges blooming will cause the distortion of the charge distribution. Commonly, the largest signal outputted from CCD sensor is restricted by capability of the collection well of CCD, and can't go beyond the dynamic range for the subsequent electron circuits maintaining normal work. So the signal is not distorted in the post-processing circuits. But some techniques in the circuit can make some dazzling effects present different phenomenon in final image.

CCD image sensor induced error in PIV applications

NASA Astrophysics Data System (ADS)

Legrand, M.; Nogueira, J.; Vargas, A. A.; Ventas, R.; Rodríguez-Hidalgo, M. C.

2014-06-01

The readout procedure of charge-coupled device (CCD) cameras is known to generate some image degradation in different scientific imaging fields, especially in astrophysics. In the particular field of particle image velocimetry (PIV), widely extended in the scientific community, the readout procedure of the interline CCD sensor induces a bias in the registered position of particle images. This work proposes simple procedures to predict the magnitude of the associated measurement error. Generally, there are differences in the position bias for the different images of a certain particle at each PIV frame. This leads to a substantial bias error in the PIV velocity measurement (˜0.1 pixels). This is the order of magnitude that other typical PIV errors such as peak-locking may reach. Based on modern CCD technology and architecture, this work offers a description of the readout phenomenon and proposes a modeling for the CCD readout bias error magnitude. This bias, in turn, generates a velocity measurement bias error when there is an illumination difference between two successive PIV exposures. The model predictions match the experiments performed with two 12-bit-depth interline CCD cameras (MegaPlus ES 4.0/E incorporating the Kodak KAI-4000M CCD sensor with 4 megapixels). For different cameras, only two constant values are needed to fit the proposed calibration model and predict the error from the readout procedure. Tests by different researchers using different cameras would allow verification of the model, that can be used to optimize acquisition setups. Simple procedures to obtain these two calibration values are also described.

Effects of biomechanical forces on signaling in the cortical collecting duct (CCD)

PubMed Central

Carrisoza-Gaytan, Rolando; Liu, Yu; Flores, Daniel; Else, Cindy; Lee, Heon Goo; Rhodes, George; Sandoval, Ruben M.; Kleyman, Thomas R.; Lee, Francis Young-In; Molitoris, Bruce; Satlin, Lisa M.

2014-01-01

An increase in tubular fluid flow rate (TFF) stimulates Na reabsorption and K secretion in the cortical collecting duct (CCD) and subjects cells therein to biomechanical forces including fluid shear stress (FSS) and circumferential stretch (CS). Intracellular MAPK and extracellular autocrine/paracrine PGE2 signaling regulate cation transport in the CCD and, at least in other systems, are affected by biomechanical forces. We hypothesized that FSS and CS differentially affect MAPK signaling and PGE2 release to modulate cation transport in the CCD. To validate that CS is a physiological force in vivo, we applied the intravital microscopic approach to rodent kidneys in vivo to show that saline or furosemide injection led to a 46.5 ± 2.0 or 170 ± 32% increase, respectively, in distal tubular diameter. Next, murine CCD (mpkCCD) cells were grown on glass or silicone coated with collagen type IV and subjected to 0 or 0.4 dyne/cm2 of FSS or 10% CS, respectively, forces chosen based on prior biomechanical modeling of ex vivo microperfused CCDs. Cells exposed to FSS expressed an approximately twofold greater abundance of phospho(p)-ERK and p-p38 vs. static cells, while CS did not alter p-p38 and p-ERK expression compared with unstretched controls. FSS induced whereas CS reduced PGE2 release by ∼40%. In conclusion, FSS and CS differentially affect ERK and p38 activation and PGE2 release in a cell culture model of the CD. We speculate that TFF differentially regulates biomechanical signaling and, in turn, cation transport in the CCD. PMID:24872319

A Novel Truncating LMNA Mutation in Patients with Cardiac Conduction Disorders and Dilated Cardiomyopathy.

PubMed

Kawakami, Hiroshi; Ogimoto, Akiyoshi; Tokunaga, Naohito; Nishimura, Kazuhisa; Kawakami, Hideo; Higashi, Haruhiko; Iio, Chiharuko; Kono, Tamami; Aono, Jun; Uetani, Teruyoshi; Nagai, Takayuki; Inoue, Katsuji; Suzuki, Jun; Ikeda, Shuntaro; Okura, Takafumi; Ohyagi, Yasumasa; Tabara, Yasuharu; Higaki, Jitsuo

2018-05-30

The cardiac phenotype of laminopathies is characterized by cardiac conduction disorders (CCDs) and dilated cardiomyopathy (DCM). Although laminopathies have been considered monogenic, they exhibit a remarkable degree of clinical variability. This case series aimed to detect the causal mutation and to investigate the causes of clinical variability in a Japanese family with inherited CCD and DCM.Of the five family members investigated, four had either CCD/DCM or CCD alone, while one subject had no cardiovascular disease and acted as a normal control. We performed targeted resequencing of 174 inherited cardiovascular disease-associated genes in this family and pathological mutations were confirmed using Sanger sequencing. The degree of clinical severity and variability were also evaluated using long-term medical records. We discovered a novel heterozygous truncating lamin A/C (LMNA) mutation (c.774delG) in all four subjects with CCD. Because this mutation was predicted to cause a frameshift mutation and premature termination (p.Gln258HisfsTer222) in LMNA, we believe that this LMNA mutation was the causal mutation in this family with CCD and laminopathies. In addition, gender-specific intra-familiar clinical variability was observed in this Japanese family where affected males exhibited an earlier onset of CCD and more severe DCM compared to affected females. Using targeted resequencing, we discovered a novel truncating LMNA mutation associated with CCD and DCM in this family characterized by gender differences in clinical severity in LMNA carriers. Our results suggest that in patients with laminopathy, clinical severity may be the result of multiple factors.

A high-resolution multimode digital microscope system.

PubMed

Salmon, Edward D; Shaw, Sidney L; Waters, Jennifer C; Waterman-Storer, Clare M; Maddox, Paul S; Yeh, Elaine; Bloom, Kerry

2013-01-01

This chapter describes the development of a high-resolution, multimode digital imaging system based on a wide-field epifluorescent and transmitted light microscope, and a cooled charge-coupled device (CCD) camera. The three main parts of this imaging system are Nikon FXA microscope, Hamamatsu C4880 cooled CCD camera, and MetaMorph digital imaging system. This chapter presents various design criteria for the instrument and describes the major features of the microscope components-the cooled CCD camera and the MetaMorph digital imaging system. The Nikon FXA upright microscope can produce high resolution images for both epifluorescent and transmitted light illumination without switching the objective or moving the specimen. The functional aspects of the microscope set-up can be considered in terms of the imaging optics, the epi-illumination optics, the transillumination optics, the focus control, and the vibration isolation table. This instrument is somewhat specialized for microtubule and mitosis studies, and it is also applicable to a variety of problems in cellular imaging, including tracking proteins fused to the green fluorescent protein in live cells. The instrument is also valuable for correlating the assembly dynamics of individual cytoplasmic microtubules (labeled by conjugating X-rhodamine to tubulin) with the dynamics of membranes of the endoplasmic reticulum (labeled with DiOC6) and the dynamics of the cell cortex (by differential interference contrast) in migrating vertebrate epithelial cells. This imaging system also plays an important role in the analysis of mitotic mutants in the powerful yeast genetic system Saccharomyces cerevisiae. Copyright © 1998 Elsevier Inc. All rights reserved.

Attenuation characteristics of fiberoptic plates for digital mammography and other X-ray imaging applications.

PubMed

Vedantham, S; Karellas, A; Suryanarayanan, S

2003-01-01

Spatially coherent fiberoptic plates are important components of some charge-coupled device (CCD)-based x-ray imaging systems. These plates efficiently transmit scintillations from the phosphor, and also filter out x-rays not absorbed by the phosphor, thus protecting the CCD from direct x-ray interaction. The thickness of the fiberoptic plate and the CCD package present a significant challenge in the design of a digital x-ray cassette capable of insertion into the existing film-screen cassette holders of digital mammography systems. This study was performed with an aim to optimize fiberoptic plate thickness. Attenuation measurements were performed on nine fiberoptic plates varying in material composition that exhibit desirable optical characteristics such as good coupling efficiency. Mammographic spectra from a clinical mammographic system and an Americium-241 (Am-241) source (59.54 KeV) were used. The spectra were recorded with a high-resolution cadmium zinc telluride (CZT)-based spectrometer and corrected for dead time and pile-up. The linear attenuation coefficients varied by a factor of 3 in the set of tested fiberoptic plates at both mammographic energies and 59.54 keV. Our results suggest that a 3-mm thick high-absorption plate might provide adequate for shielding at mammographic energies. A thickness of 2-mm is feasible for mammographic applications with further optimization of the fiberoptic plate composition by incorporating non-scintillating, high-atomic number material. This would allow more space for cooling components of the cassette and for a more compact device, which is critical for clinical implementation of the technology.

X-ray phase contrast imaging at MAMI

NASA Astrophysics Data System (ADS)

El-Ghazaly, M.; Backe, H.; Lauth, W.; Kube, G.; Kunz, P.; Sharafutdinov, A.; Weber, T.

2006-05-01

Experiments have been performed to explore the potential of the low emittance 855MeV electron beam of the Mainz Microtron MAMI for imaging with coherent X-rays. Transition radiation from a micro-focused electron beam traversing a foil stack served as X-ray source with good transverse coherence. Refraction contrast radiographs of low absorbing materials, in particular polymer strings with diameters between 30 and 450μm, were taken with a polychromatic transition radiation X-ray source with a spectral distribution in the energy range between 8 and about 40keV. The electron beam spot size had standard deviation σh = (8.6±0.1)μm in the horizontal and σv = (7.5±0.1)μm in the vertical direction. X-ray films were used as detectors. The source-to-detector distance amounted to 11.4m. The objects were placed in a distance of up to 6m from the X-ray film. Holograms of strings were taken with a beam spot size σv = (0.50±0.05)μm in vertical direction, and a monochromatic X-ray beam of 6keV energy. A good longitudinal coherence has been obtained by the (111) reflection of a flat silicon single crystal in Bragg geometry. It has been demonstrated that a direct exposure CCD chip with a pixel size of 13×13μm^2 provides a highly efficient on-line detector. Contrast images can easily be generated with a complete elimination of all parasitic background. The on-line capability allows a minimization of the beam spot size by observing the smallest visible interference fringe spacings or the number of visible fringes. It has been demonstrated that X-ray films are also very useful detectors. The main advantage in comparison with the direct exposure CCD chip is the resolution. For the Structurix D3 (Agfa) X-ray film the standard deviation of the resolution was measured to be σf = (1.2±0.4)μm, which is about a factor of 6 better than for the direct exposure CCD chip. With the small effective X-ray spot size in vertical direction of σv = (1.2±0.3)μm and a geometrical magnification of up to 7.4 high-quality holograms of tiny transparent strings were taken in which the holographic information is contained in up to 18 interference fringes.

Electronic pictures from charged-coupled devices

NASA Technical Reports Server (NTRS)

Mccann, D. H.; Turly, A. P.; White, M.

1979-01-01

Imaging system uses charge-coupled devices (CCD's) to generate TV-like pictures with high resolution, sensitivity, and signal-to-noise ratio. It combines detectors for five spectral bands as well as processing and control circuitry all on single silicon chip.

Measurement of material mechanical properties in microforming

NASA Astrophysics Data System (ADS)

Yun, Wang; Xu, Zhenying; Hui, Huang; Zhou, Jianzhong

2006-02-01

As the rapid market need of micro-electro-mechanical systems engineering gives it the wide development and application ranging from mobile phones to medical apparatus, the need of metal micro-parts is increasing gradually. Microforming technology challenges the plastic processing technology. The findings have shown that if the grain size of the specimen remains constant, the flow stress changes with the increasing miniaturization, and also the necking elongation and the uniform elongation etc. It is impossible to get the specimen material properties in conventional tensile test machine, especially in the high precision demand. Therefore, one new measurement method for getting the specimen material-mechanical property with high precision is initiated. With this method, coupled with the high speed of Charge Coupled Device (CCD) camera and high precision of Coordinate Measuring Machine (CMM), the elongation and tensile strain in the gauge length are obtained. The elongation, yield stress and other mechanical properties can be calculated from the relationship between the images and CCD camera movement. This measuring method can be extended into other experiments, such as the alignment of the tool and specimen, micro-drawing process.

Dual crystal x-ray spectrometer at 1.8 keV for high repetition-rate single-photon counting spectroscopy experiments

DOE PAGES

Gamboa, E. J.; Bachmann, B.; Kraus, D.; ...

2016-08-01

The recent development of high-repetition rate x-ray free electron lasers (FEL), makes it possible to perform x-ray scattering and emission spectroscopy measurements from thin foils or gasses heated to high-energy density conditions by integrating over many experimental shots. Since the expected signal may be weaker than the typical CCD readout noise over the region-of-interest, it is critical to the success of this approach to use a detector with high-energy resolution so that single x-ray photons may be isolated. We describe a dual channel x-ray spectrometer developed for the Atomic and Molecular Optics endstation at the Linac Coherent Light Source (LCLS)more » for x-ray spectroscopy near the K-edge of aluminum. The spectrometer is based on a pair of curved PET (002) crystals coupled to a single pnCCD detector which simultaneously measures x-ray scattering and emission in the forward and backward directions. Furthermore, the signals from single x-ray photons are accumulated permitting continuous single-shot acquisition at 120 Hz.« less

MMW/THz imaging using upconversion to visible, based on glow discharge detector array and CCD camera

NASA Astrophysics Data System (ADS)

Aharon, Avihai; Rozban, Daniel; Abramovich, Amir; Yitzhaky, Yitzhak; Kopeika, Natan S.

2017-10-01

An inexpensive upconverting MMW/THz imaging method is suggested here. The method is based on glow discharge detector (GDD) and silicon photodiode or simple CCD/CMOS camera. The GDD was previously found to be an excellent room-temperature MMW radiation detector by measuring its electrical current. The GDD is very inexpensive and it is advantageous due to its wide dynamic range, broad spectral range, room temperature operation, immunity to high power radiation, and more. An upconversion method is demonstrated here, which is based on measuring the visual light emitting from the GDD rather than its electrical current. The experimental setup simulates a setup that composed of a GDD array, MMW source, and a basic CCD/CMOS camera. The visual light emitting from the GDD array is directed to the CCD/CMOS camera and the change in the GDD light is measured using image processing algorithms. The combination of CMOS camera and GDD focal plane arrays can yield a faster, more sensitive, and very inexpensive MMW/THz camera, eliminating the complexity of the electronic circuits and the internal electronic noise of the GDD. Furthermore, three dimensional imaging systems based on scanning prohibited real time operation of such imaging systems. This is easily solved and is economically feasible using a GDD array. This array will enable us to acquire information on distance and magnitude from all the GDD pixels in the array simultaneously. The 3D image can be obtained using methods like frequency modulation continuous wave (FMCW) direct chirp modulation, and measuring the time of flight (TOF).

The first US Naval Observatory CCD Astrograph Catalog

NASA Astrophysics Data System (ADS)

Germain, M. E.; Zacharias, N.; Urban, S. E.; Rafferty, T. J.; Holdenried, E. R.; Zacharias, M. I.; Hall, D. M.; Wycoff, G. L.; Monet, D. G.

2000-05-01

The USNO CCD Astrograph Catalog (UCAC) project is a high precision, astrometric survey of stars having R magnitudes between 7th and 16th. The positional accuracy is 20 mas for stars between 9th and 14th, and 70 mas for fainter stars. This gives a density (stars per square degree) higher than that of the Guide Star Catalog (GSC), with an improvement in positional accuracy of about a factor of ten. Observations began in January 1998 at Cerro Tololo Inter--American Observatory (CTIO) using a five-element 0.2 meter astrograph equipped with a 4k by 4k CCD. The instrument will be moved north in early 2001, and full sky coverage is expected by early 2003. A preliminary catalog (UCAC1) of positions and proper motions of 27 million stars has been constructed which is available on CD-ROM from USNO. Observations between 13 Feb 1998 and 07 Nov 1999 are included with a total of over 79,000 CCD frames covering 80% of the Southern Hemisphere. The catalog is on the International Celestial Reference System (ICRS), which is consistent with J2000. Proper motions of bright stars (V <= 12.5) were derived using a combination of ground-based astrometric catalogs, Hipparcos, and Tycho-2 positions, giving a typical error of 3 mas/yr. For the fainter stars the USNO A2.0 (Monet, 1998) was used as first epoch, with typical proper motion errors of 10 to 15 mas/yr. External comparisons with Tycho-2 and the Yale Southern Proper Motion (SPM) 2.0 data reveal systematic errors to be only on the 10 mas level.

Critical Conditions for Liquid Chromatography of Statistical Copolymers: Functionality Type and Composition Distribution Characterization by UP-LCCC/ESI-MS.

PubMed

Epping, Ruben; Panne, Ulrich; Falkenhagen, Jana

2017-02-07

Statistical ethylene oxide (EO) and propylene oxide (PO) copolymers of different monomer compositions and different average molar masses additionally containing two kinds of end groups (FTD) were investigated by ultra high pressure liquid chromatography under critical conditions (UP-LCCC) combined with electrospray ionization time-of flight mass spectrometry (ESI-TOF-MS). Theoretical predictions of the existence of a critical adsorption point (CPA) for statistical copolymers with a given chemical and sequence distribution1 could be studied and confirmed. A fundamentally new approach to determine these critical conditions in a copolymer, alongside the inevitable chemical composition distribution (CCD), with mass spectrometric detection, is described. The shift of the critical eluent composition with the monomer composition of the polymers was determined. Due to the broad molar mass distribution (MMD) and the presumed existence of different end group functionalities as well as monomer sequence distribution (MSD), gradient separation only by CCD was not possible. Therefore, isocratic separation conditions at the CPA of definite CCD fractions were developed. Although the various present distributions partly superimposed the separation process, the goal of separation by end group functionality was still achieved on the basis of the additional dimension of ESI-TOF-MS. The existence of HO-H besides the desired allylO-H end group functionalities was confirmed and their amount estimated. Furthermore, indications for a MSD were found by UPLC/MS/MS measurements. This approach offers for the first time the possibility to obtain a fingerprint of a broad distributed statistical copolymer including MMD, FTD, CCD, and MSD.

Modelling electron distributions within ESA's Gaia satellite CCD pixels to mitigate radiation damage

NASA Astrophysics Data System (ADS)

Seabroke, G. M.; Holland, A. D.; Burt, D.; Robbins, M. S.

2009-08-01

The Gaia satellite is a high-precision astrometry, photometry and spectroscopic ESA cornerstone mission, currently scheduled for launch in 2012. Its primary science drivers are the composition, formation and evolution of the Galaxy. Gaia will achieve its unprecedented positional accuracy requirements with detailed calibration and correction for radiation damage. At L2, protons cause displacement damage in the silicon of CCDs. The resulting traps capture and emit electrons from passing charge packets in the CCD pixel, distorting the image PSF and biasing its centroid. Microscopic models of Gaia's CCDs are being developed to simulate this effect. The key to calculating the probability of an electron being captured by a trap is the 3D electron density within each CCD pixel. However, this has not been physically modelled for the Gaia CCD pixels. In Seabroke, Holland & Cropper (2008), the first paper of this series, we motivated the need for such specialised 3D device modelling and outlined how its future results will fit into Gaia's overall radiation calibration strategy. In this paper, the second of the series, we present our first results using Silvaco's physics-based, engineering software: the ATLAS device simulation framework. Inputting a doping profile, pixel geometry and materials into ATLAS and comparing the results to other simulations reveals that ATLAS has a free parameter, fixed oxide charge, that needs to be calibrated. ATLAS is successfully benchmarked against other simulations and measurements of a test device, identifying how to use it to model Gaia pixels and highlighting the affect of different doping approximations.

The fast and accurate 3D-face scanning technology based on laser triangle sensors

NASA Astrophysics Data System (ADS)

Wang, Jinjiang; Chang, Tianyu; Ge, Baozhen; Tian, Qingguo; Chen, Yang; Kong, Bin

2013-08-01

A laser triangle scanning method and the structure of 3D-face measurement system were introduced. In presented system, a liner laser source was selected as an optical indicated signal in order to scanning a line one times. The CCD image sensor was used to capture image of the laser line modulated by human face. The system parameters were obtained by system calibrated calculated. The lens parameters of image part of were calibrated with machine visual image method and the triangle structure parameters were calibrated with fine wire paralleled arranged. The CCD image part and line laser indicator were set with a linear motor carry which can achieve the line laser scanning form top of the head to neck. For the nose is ledge part and the eyes are sunk part, one CCD image sensor can not obtain the completed image of laser line. In this system, two CCD image sensors were set symmetric at two sides of the laser indicator. In fact, this structure includes two laser triangle measure units. Another novel design is there laser indicators were arranged in order to reduce the scanning time for it is difficult for human to keep static for longer time. The 3D data were calculated after scanning. And further data processing include 3D coordinate refine, mesh calculate and surface show. Experiments show that this system has simply structure, high scanning speed and accurate. The scanning range covers the whole head of adult, the typical resolution is 0.5mm.

CCD Astrometry with Robotic Telescopes

NASA Astrophysics Data System (ADS)

AlZaben, Faisal; Li, Dewei; Li, Yongyao; Dennis, Aren Fene, Michael; Boyce, Grady; Boyce, Pat

2016-01-01

CCD images were acquired of three binary star systems: WDS06145+1148, WDS06206+1803, and WDS06224+2640. The astrometric solution, position angle, and separation of each system were calculated with MaximDL v6 and Mira Pro x64 software suites. The results were consistent with historical measurements in the Washington Double Star Catalog. Our analysis found some differences in measurements between single-shot color CCD cameras and traditional monochrome CCDs using a filter wheel.

A FORTRAN version implementation of block adjustment of CCD frames and its preliminary application

NASA Astrophysics Data System (ADS)

Yu, Y.; Tang, Z.-H.; Li, J.-L.; Zhao, M.

2005-09-01

A FORTRAN version implementation of the block adjustment (BA) of overlapping CCD frames is developed and its flowchart is shown. The program is preliminarily applied to obtain the optical positions of four extragalactic radio sources. The results show that because of the increase in the number and sky coverage of reference stars the precision of optical positions with BA is improved compared with the single CCD frame adjustment.